LOCUS       DAOSUG010000001        17343 bp    DNA     linear   ENV 10-JUN-2024
DEFINITION  MAG TPA_asm: Micropruina sp. isolate AMR_MDS_2195
            NODE_2048_length_17343_cov_4.506421, whole genome shotgun sequence.
ACCESSION   DAOSUG010000001 DAOSUG010000000
VERSION     DAOSUG010000001.1
DBLINK      BioProject: PRJNA925477
            BioSample: SAMN32864262
            Sequence Read Archive: SRR4236673
KEYWORDS    WGS; ENV; Metagenome Assembled Genome; MAG; Third Party Data; TPA;
            TPA:assembly.
SOURCE      Micropruina sp. (wastewater metagenome)
  ORGANISM  Micropruina sp.
            Bacteria; Bacillati; Actinomycetota; Actinomycetes;
            Propionibacteriales; Nocardioidaceae; Micropruina.
REFERENCE   1  (bases 1 to 17343)
  AUTHORS   Abdulkadir,N., Saraiva,J.P., Zhang,J., Stolte,S., Gillor,O.,
            Harms,H. and Rocha,U.
  TITLE     Genome-centric analyses of 165 metagenomes show that mobile genetic
            elements are crucial for the transmission of antimicrobial
            resistance genes to pathogens in activated sludge and wastewater
  JOURNAL   Microbiol Spectr 12 (3), e0291823 (2024)
   PUBMED   38289113
REFERENCE   2  (bases 1 to 17343)
  AUTHORS   Abdulkadir,N.
  TITLE     Direct Submission
  JOURNAL   Submitted (24-MAR-2023) Environmental Microbiology, Helmholtz
            Center for Environmental Research -UFZ, Permoserstr 15, Leipzig
            04318, Germany
COMMENT     The annotation was added by the NCBI Prokaryotic Genome Annotation
            Pipeline (PGAP). Information about PGAP can be found here:
            https://www.ncbi.nlm.nih.gov/genome/annotation_prok/
            
            ##Genome-Assembly-Data-START##
            Assembly Method        :: SPAdes v. 3.11.1
            Genome Representation  :: Full
            Expected Final Version :: Yes
            Genome Coverage        :: Not Applicable
            Sequencing Technology  :: Illumina MiSeq
            ##Genome-Assembly-Data-END##
            
            ##Genome-Annotation-Data-START##
            Annotation Provider               :: NCBI
            Annotation Date                   :: 05/04/2023 04:57:10
            Annotation Pipeline               :: NCBI Prokaryotic Genome
                                                 Annotation Pipeline (PGAP)
            Annotation Method                 :: Best-placed reference protein
                                                 set; GeneMarkS-2+
            Annotation Software revision      :: 6.5
            Features Annotated                :: Gene; CDS; rRNA; tRNA; ncRNA
            Genes (total)                     :: 2,776
            CDSs (total)                      :: 2,750
            Genes (coding)                    :: 2,714
            CDSs (with protein)               :: 2,714
            Genes (RNA)                       :: 26
            tRNAs                             :: 24
            ncRNAs                            :: 2
            Pseudo Genes (total)              :: 36
            CDSs (without protein)            :: 36
            Pseudo Genes (ambiguous residues) :: 0 of 36
            Pseudo Genes (frameshifted)       :: 2 of 36
            Pseudo Genes (incomplete)         :: 32 of 36
            Pseudo Genes (internal stop)      :: 2 of 36
            ##Genome-Annotation-Data-END##
FEATURES             Location/Qualifiers
     source          1..17343
                     /organism="Micropruina sp."
                     /mol_type="genomic DNA"
                     /submitter_seqid="NODE_2048_length_17343_cov_4.506421"
                     /isolate="AMR_MDS_2195"
                     /isolation_source="Activated sludge of municipal
                     wastewater treatment plants"
                     /db_xref="taxon:2737536"
                     /environmental_sample
                     /geo_loc_name="Canada"
                     /lat_lon="49.25 N 123.1 W"
                     /collection_date="2015-06-16"
                     /metagenome_source="wastewater metagenome"
                     /note="metagenomic"
     gene            <1..186
                     /locus_tag="PLF56_00005"
     CDS             <1..186
                     /locus_tag="PLF56_00005"
                     /inference="COORDINATES: ab initio
                     prediction:GeneMarkS-2+"
                     /note="Derived by automated computational analysis using
                     gene prediction method: GeneMarkS-2+."
                     /codon_start=1
                     /transl_table=11
                     /product="type II secretion protein F"
                     /protein_id="HOQ51975.1"
                     /translation="LAPLLRQHAREAQHAVHALAQARARRAGVSSVLPLMVCFLPAFI
                     LIGVVPIVGGVAARLFS"
     gene            331..531
                     /locus_tag="PLF56_00010"
     CDS             331..531
                     /locus_tag="PLF56_00010"
                     /inference="COORDINATES: protein motif:HMM:NF025395.2"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="DUF4244 domain-containing protein"
                     /protein_id="HOQ51976.1"
                     /translation="MSMQTLAVPRSERGMTTAEYALGTIAVVSLVGVIISVITGGGLS
                     KEISQVVNQIIDWIQDFIKKQK"
     gene            1035..1352
                     /locus_tag="PLF56_00015"
     CDS             1035..1352
                     /locus_tag="PLF56_00015"
                     /inference="COORDINATES: ab initio
                     prediction:GeneMarkS-2+"
                     /note="Derived by automated computational analysis using
                     gene prediction method: GeneMarkS-2+."
                     /codon_start=1
                     /transl_table=11
                     /product="hypothetical protein"
                     /protein_id="HOQ51977.1"
                     /translation="MVTVEVAFAVMLSAVVALALCYLIALVVQLGQLQAVAGEVARQH
                     ARSDTAAARRAADDAPFGTVVRVSNSGSDVVVVAELKSQPWGRWIPAMSLSARAVVLK
                     EGV"
     gene            1502..1840
                     /locus_tag="PLF56_00020"
     CDS             1502..1840
                     /locus_tag="PLF56_00020"
                     /inference="COORDINATES: protein motif:HMM:TIGR03816.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="hypothetical protein"
                     /protein_id="HOQ51978.1"
                     /translation="MLAVATAMALLTVTAVGVLGMTYAATARSVRAAADLVAVSGAQA
                     NGQGSDACDEAKRIAARNQVVLSGCAVTGDLIDYVVEVTVRRDVGWRLPGLPERVSAT
                     AYAGSVSGVP"
     gene            complement(1810..4089)
                     /locus_tag="PLF56_00025"
     CDS             complement(1810..4089)
                     /locus_tag="PLF56_00025"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_013161894.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_function: GO:0003676 - nucleic acid binding [Evidence
                     IEA];
                     GO_function: GO:0005524 - ATP binding [Evidence IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="DEAD/DEAH box helicase"
                     /protein_id="HOQ51979.1"
                     /translation="MAVPDWISNEPQVRHVHHRPARSGVTADLPDWVSARARQTLAEQ
                     GITELWQHQQVAAQAAFDGRHVAISTSTASGKSLAYLLPIHAATAEGTLRPGVAPHRW
                     ATAARAHTALYLAPTKALAHDQARRAREFGGSAWRLTTLDGDSEAEERRFAREFATFV
                     LTNPDMLHRSVLPNHERWQGLLRSLRYVVIDEAHRYRGVFGAHVASVLRRLRRLCRHY
                     GADPVFISASGTIAAPAQHLQRLAGVDPVLAVETDTSTRPALDFVIWQGDGDAHREAA
                     GMLTRLVDTGGQVLAFTTSRVQAELVAEQARRHSIDPDGIASYRAGYLAADRRALEAA
                     LSCGRLRGVACTNALELGVDISGMDAVLSIGFPGSLAALWQQAGRAGRDGRDALAVLI
                     ARPDPLDTWLCDHPEALFDTPVETTVLHPGQPAVLAAHLAAAAQELAVTADDQQFFGP
                     GIPPLLEHLTTRGVLRRRGSAWYWTSQHRAVDAIDLRSIGGTGIEIVQSGTGRVIGQV
                     DASAADRTVHEGAVYLHQGEQWLVESYDLRAGVALCRQQRAEWFTQAQGSTDVRVVGT
                     DRRREIGSGSLFAGTIELTSQVTGYLRRDAVTGSVWDSTPLDLPARTFRTRATWFTLH
                     ERDRALHGLDDRALAAGLHAAEHAAIGLLPGFAPCDKGDVGGLSTVIHPDTGAPTIFV
                     HDGLPGGSGFADEAYRVAERWLAATLDLLRACECEQGCPRCVVSPACGSGNHPLDRRA
                     AIAVLQVLVHHGTPLTLPA"
     gene            complement(4529..6181)
                     /locus_tag="PLF56_00030"
     CDS             complement(4529..6181)
                     /locus_tag="PLF56_00030"
                     /inference="COORDINATES: protein motif:HMM:NF019581.2"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="amidohydrolase family protein"
                     /protein_id="HOQ51980.1"
                     /translation="MSGSVLVRRARLVDLDGARAGSAASGARPDVGSGAPVDVLIVDG
                     VIDRVGVEVRAPSGVEVIDAAGRWAIPGLWDAHVHAGQWVRTARMLPLGAAGCAADVL
                     AVVAAAAPSVPSGRVVLGFGYRAAGWPRPGTVAELDAVSDGRAVALVSGDAHTGWLNS
                     RALQLLGAAPRIGPVSENEWFELLRRLDELPGAAPTPADFVAPLAGLAALGVTGLVDF
                     EFENSFVDWPVRIAAGADSVRVRAGVYPHQLRGVIDAGLRTGDALPVPGQRSVARGAG
                     SPHSATARTGAVAGRQPSTPDRLPVDRGLVTMGPLKIISDGSLGSRTAWTHEPYTDGA
                     ASPDHPCGQANYSAADLVGLLAQARECGLEVALHAIGDRAVGAAIDAFAATGARGSIE
                     HAQLISRADARRLATLGVRASVQPAHLLDDRDLTDRVWGERGSMAFAVRDLLRAGVDL
                     RLGSDAPVAPLDPWLAMATAVHRSGDERPAWHPEQSITPREAMAASVDGRRLTPGAPG
                     DLVLLDRDPLAVADDPRAAAGGLCTTRSTLTVCAGRITHRTL"
     gene            6200..7741
                     /locus_tag="PLF56_00035"
     CDS             6200..7741
                     /locus_tag="PLF56_00035"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_007079453.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_function: GO:0008168 - methyltransferase activity
                     [Evidence IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="methyltransferase"
                     /protein_id="HOQ51981.1"
                     /translation="MLDRTDARRLADALRAAGYTYDEVAARLGTEALDGLARNATLPA
                     DRALAGADDPQALLIRCFALQQPVPTAGLRAAIGEIVDRPELVRPADTTRVDRPTLVR
                     AADTTARTGPHGSSEAAIEIRPYAFDDADGEHAGWLASDLTPILDGRVARPRADFVLG
                     LSPASVTLAQLTMRTPVARALDLGTGCGVQSVHLATHAAAVVATDLNPRACAMTRLTA
                     DLNAVDLDVRQGSLYEPVAEDTFDLIVTNPPYVMSPPQGERLVYREGTHTADGLMHAV
                     VTGAPARLNPGGALQVLGNWAITADQPWQDRLAGWLEPTGCDALVLQRERLDPYEYIE
                     LWLADAGLVGTPDYARRYAEWAGYFDALGIIGVGLGWLALYRNERDQPHLTIQDWPHQ
                     VHQPVAGAFAEFTGAVTAARRSDAELLAGTLTTAPDLTQETLGRPGAADPEHIVLRRT
                     AGFGRAVEVDAALAAVIGACDGDLPLGMLIEAVGGLLEVNAADLVAELLPRLRTLIAD
                     GYLRG"
     gene            7846..10620
                     /gene="topA"
                     /locus_tag="PLF56_00040"
     CDS             7846..10620
                     /gene="topA"
                     /locus_tag="PLF56_00040"
                     /EC_number="5.6.2.1"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_013865799.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_component: GO:0005737 - cytoplasm [Evidence IEA];
                     GO_function: GO:0003677 - DNA binding [Evidence IEA];
                     GO_function: GO:0003917 - DNA topoisomerase type I (single
                     strand cut, ATP-independent) activity [Evidence IEA];
                     GO_process: GO:0006260 - DNA replication [Evidence IEA];
                     GO_process: GO:0006265 - DNA topological change [Evidence
                     IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="type I DNA topoisomerase"
                     /protein_id="HOQ51982.1"
                     /translation="MATAGKKALVIVESPAKATKIASFLGNSYVVESSRGHVADLPTG
                     ASEVPAKYKGEKWARTGVNVDNNFEPLYVVQADKKGTIRELKAKLATADELLLATDED
                     REGEAIAWHLLQQLKPKVPVRRMVFHEITPQAIAEAVANPRELDTDLVDAQETRRILD
                     RLYGYEVSPVLWKKVMPKLSAGRVQSVATRLVVDRERERIAFRSAGYAGIEAVLNAGD
                     QAEPPTFKATLQQVSDLRVAAGRDFDAQGKLTGKGLLHLSAEQAKSLAEVLESASYAV
                     ASVEAKPYTRRPYAPFRTTTLQQDGGRKLGFTAERTMRVAQDLYERGFITYMRTDSVT
                     LSASAITAARAQVKQLFGGQYLPDQPRVYTSKVKNAQEAHEAIRPAGEHFAAPAETGL
                     SGDAFRLYELIWKRTLASQMKDAVGETVTVRIGATLPQRLSITDATTGEITETGRCTF
                     GASGRTITFPGFLKAYVVGVDDDETSDDAQARLPQLVAGAPLSADEVKAVSHDTRPPA
                     RYTEPSLVAKLEELEIGRPSTYASIIRTITSRDYVFKKGSALVPTWLAFAVTRLLEEH
                     FPKLVDYTFTAALEETLDDIANGQAARLQVLSDFYFGPEGSAHEGLTTLVNELGEIDA
                     RELSTHPLGDSGINVRVGRYGTYVEDPDGRRANVPDDLPPDELTAERARELLEQPMGE
                     ERELGVDPASGHTVVAKNGRYGPYVTETLPEDAPKSAKPRTGSLFASMTLDTVTLDDA
                     LKLMSLPRVVGVSTDGVEITAQNGRYGPYLKKGTDSRSLTSEEQIFGITLDEAEAIYA
                     QPKTRGARTAAAPLKELGADPVSGKPMVIKDGRFGAYVTDGEVNATLRKSDSIEAITA
                     ERAAELLAEKRAKGPAPKRTTRRAPAKRATGAQASGTKAAGTKATGAKASGTATKRAA
                     NTNSATKRGG"
     gene            10626..12089
                     /locus_tag="PLF56_00045"
     CDS             10626..12089
                     /locus_tag="PLF56_00045"
                     /inference="COORDINATES: ab initio
                     prediction:GeneMarkS-2+"
                     /note="Derived by automated computational analysis using
                     gene prediction method: GeneMarkS-2+."
                     /codon_start=1
                     /transl_table=11
                     /product="hypothetical protein"
                     /protein_id="HOQ51983.1"
                     /translation="MTDLNDEVLRVDLPPAAAVANLTGPATGIHRLVARPPVQTTAIV
                     IDTDDHRLLDWSIELSRITETGQWVLRAPLWAPPLQAETISPQAEDDLPVDLADVLVP
                     FRRGGILGPRLSVETQRRQFTLEGSDGAVIGELSDERHRIGSHAGQMTAYRNVTLKLA
                     RPRPEHLQAITAAFVAAGGVVVTGFEPLAVRLGLSHHGRGRSALSAKVPIGDYVRAQF
                     ESRWRRLLRADLEARTGSGDDGALRENVAALLQELVGLEPLLDADWAEQAKQHAEAAS
                     ETTRPLQHTQRWLRILDLLATASTSPPVRVAAERLTGPVLAQELEAVVQTLLDQCRTL
                     EPYSEDARWSRAHQLAGRAVTLSMLARDVFGKPAKQLRKHLSPISEALAHTLRPDPVA
                     LKRDLHGLSTAEIFAAGRAYERTVLSVDYARESFVREWPQLGEALRPHVIRPRAPHTP
                     ARPGAVAARPAAPGRAVEPASDASVPIPAATSAAVPS"
     gene            12086..12721
                     /gene="tmk"
                     /locus_tag="PLF56_00050"
     CDS             12086..12721
                     /gene="tmk"
                     /locus_tag="PLF56_00050"
                     /EC_number="2.7.4.9"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_007433957.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_function: GO:0004798 - thymidylate kinase activity
                     [Evidence IEA];
                     GO_process: GO:0015949 - nucleobase-containing small
                     molecule interconversion [Evidence IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="dTMP kinase"
                     /protein_id="HOQ51984.1"
                     /translation="MSGVFVVFEGGDGVGKSTQTKLLAEWAIAQGRTVRVTFEPGDTA
                     VGRSVRSIVLDPATGDLDARAEALLYAADKAQHVHEVVRPALRRGELVVCDRYIDSML
                     AYQGAGRVLDLDEVAGIAEWATDGLVPDLTVLLDLDPAEAVGAIEQPDRLEQAGADLH
                     QRARRFFLELASREPERYLVLPAREPRDRIAAQVQQRVGELLSSAAGRIAT"
     gene            12718..13857
                     /locus_tag="PLF56_00055"
     CDS             12718..13857
                     /locus_tag="PLF56_00055"
                     /EC_number="2.7.7.7"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_015071548.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_component: GO:0009360 - DNA polymerase III complex
                     [Evidence IEA];
                     GO_function: GO:0003677 - DNA binding [Evidence IEA];
                     GO_function: GO:0003887 - DNA-directed DNA polymerase
                     activity [Evidence IEA];
                     GO_function: GO:0008408 - 3'-5' exonuclease activity
                     [Evidence IEA];
                     GO_process: GO:0006260 - DNA replication [Evidence IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="DNA polymerase III subunit delta'"
                     /protein_id="HOQ51985.1"
                     /translation="MSVWADLVGQQRVVDVLRRAVAGGPHAMTHAWLITGPPGSGRSN
                     AARSFAAALQCAEGGCGVCSSCRTVLSGAHPDVTLVRTEQLSIGVDEVRELVRRAAMS
                     PTIGRYQVLVVEDADRVTERGADALLKSIEEPAPRTVWLLCAPTSDDVVVTIRSRCRA
                     VHLATPSNAAVTELLVRRDGVPRELAAFAARVAQGHIGRARMLARHEAVRIRRSDLLK
                     LPARLVSLPDCLTAAANLVEAAADEAAATTADLDAREKAELSEALGFGTKGARPRNAQ
                     AALRELDDQQKARAKPLQRDALDRVLTELTTYYRDVLCVQTGVGAELINAEVRPEIEA
                     MARQHAPERTLRRIDAILECRTALEGNVAPLLAMESLMISIAVSI"
     gene            14290..14793
                     /locus_tag="PLF56_00060"
     CDS             14290..14793
                     /locus_tag="PLF56_00060"
                     /inference="COORDINATES: ab initio
                     prediction:GeneMarkS-2+"
                     /note="Derived by automated computational analysis using
                     gene prediction method: GeneMarkS-2+."
                     /codon_start=1
                     /transl_table=11
                     /product="hypothetical protein"
                     /protein_id="HOQ51986.1"
                     /translation="MSHTVDDEQTSPYSPEAAAGEARTPTLPQPPRFDDLTPEEANRV
                     RLISEQFAPKLSDLQEGQDAPQAASVRPDQHAQQAASAWPVQHAQQAASVQSGQDAPQ
                     AASAWPDQAAQQAASAWPDQAAQQAPSAQPQLYAPQVASPQTGQADQPTRQQAVVGQP
                     APAPQQD"
     gene            14797..15576
                     /locus_tag="PLF56_00065"
     CDS             14797..15576
                     /locus_tag="PLF56_00065"
                     /inference="COORDINATES: protein motif:HMM:NF019301.2"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_component: GO:0016020 - membrane [Evidence IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="DoxX family protein"
                     /protein_id="HOQ51987.1"
                     /translation="MNRPQPAVEAAARPQQPVDNQATRPQPAVEQAAAAAGVNEQATR
                     PQPAVVAHQNDSIFRAQSVATPSDPPSAEQQKLDAERAARREAREQALAQPDVRTVAA
                     PEPIVVTRRTTDRFLGSFALFVLRLVVAAILAVRGLGIVTDLTSAEALFATTILPEPR
                     IAAIVTGSAALAIAVALVFGLLTRVAGLGVMLIAGGALAFVLWGNWSPFVAGRAGFIG
                     ELELLLAAVGLVFLCVGAGGFSLDRSFRKSREKDRPADQAL"
     gene            15621..17240
                     /locus_tag="PLF56_00070"
     CDS             15621..17240
                     /locus_tag="PLF56_00070"
                     /inference="COORDINATES: protein motif:HMM:NF012770.2"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_function: GO:0016787 - hydrolase activity [Evidence
                     IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="alpha/beta hydrolase"
                     /protein_id="HOQ51988.1"
                     /translation="MRTLFRWAAAAVGLTMLAGCTPYLPTSPTASSSPATSAPATGAA
                     SPSATAPAGAVITVSSVPGLEIDRPRLADPSVEPEGFADAPAGEGLAGYLDQRVRWST
                     CAQEYQCAQILAPLDYANPSEQAITLALAKKPATKSPRLGTLFVNPGGPGASGRNLVT
                     YFASAGLEQYDIVGWDPRGTNASTPVQCFDDEQTEAFTRLDSSPDDAAETEALIVGTY
                     DFAQSCWQHSGRLLQHISTVDTVRDLDLMRELLGDKLLSYVGYSYGTQIGSYYADLFP
                     QRVGRLVLDAAVDITDDDSVIQAMGFDLALGNFATWCARQGCTLGTSKDAVLKSITDW
                     LDRLDSAPVEVGDRTLTQSLAAAGVASYLYGGVPAWKSLSADLEAAMDGRGANLLKAA
                     DSLNDRNQDGSFGSLFYSFPAIGCADGYDKGVTEAEQRWVADQKKAPIFGKYFGPGYT
                     CPLWPVHSTIQFKPTGSGAAPIVVVGATGDPATPYQNAVTMAKQLKSGVLVTYEGEGH
                     GTFGGKSTCVDALVIDYLTKGTVPTDGVRCQ"
BASE COUNT         2654 a         6096 c         6115 g         2478 t
ORIGIN      
        1 ctggcgccgc tgctgcgcca gcacgcgcgc gaggcccagc acgccgtcca cgccttggcg
       61 caggcgcgtg ctcgcagggc gggggtcagc agtgtgctgc cgctgatggt ctgctttctg
      121 cccgctttca ttctcatcgg ggtggtgccg atcgtgggtg gggtggcagc ccggttgttc
      181 tcgtgacgtc gcggtggctc ggtgaacaca gctccgaccc ggttggcaag ggctttccac
      241 aaggcggtcg acgacattga acactcggcg gatctcgggc ccaagctgaa agtgagcccc
      301 ggggtggccg gggccgaaag gagcacagca atgtccatgc agacgttggc cgttccgcgt
      361 agtgaacgcg gcatgaccac ggccgagtac gcgctgggaa cgatcgcagt ggtgtcgctg
      421 gtcggggtga tcatctcggt catcaccgga ggtggcctca gcaaagagat ctcccaggtg
      481 gtgaaccaga tcatcgactg gatccaggac ttcatcaaga agcagaagtg atccgagtgg
      541 ggcggcgtcc ggccgcccca cgcgggcgct gggtaggagg aaccatgcga tcgagaaaca
      601 gccgagggtg ggtggtgggt gggcaggccg caacggtcgc ccccgcagtc ttgcccggct
      661 ctgcgatggt gcccggtaca gcggcggtgc ccggtacagc gacggtgccg ggcaatgtgg
      721 cagtttcatg cacggcacac gcgagcggca agccaggggt gcccggcgcg gcgccggctt
      781 gggccgccgc gacggtgccc ggctctgaat gggttccgga atctacgacg gagccgggat
      841 ccgcaagagt gtttggtgct gcgatgatgc ccggcactac gactgttccc ggatcgatgg
      901 ggtcggcggg ctcccggcgc gccccgcgga gccatggctt cgtgcggacc gatggtgcgg
      961 ttggctcgac gggtgccggc tcgggccgtg acgacgtcgt tggtgtgccc cgtacacctc
     1021 gggcggagcg cggcatggtc acggtcgagg tggcgttcgc ggtgatgctt tcggcggtcg
     1081 ttgccttggc gctgtgctac ttgatcgctc tggtggtgca gttgggccag ctgcaggcag
     1141 tcgcaggcga ggtggctcgt cagcatgctc gcagcgacac ggccgctgct cgacgcgcgg
     1201 ccgacgacgc accgttcggc acggtggtgc gggtcagcaa cagcggctcc gacgtggtcg
     1261 tggtggccga actcaagtcc cagccctggg ggcgttggat tccagccatg tcgttgtcgg
     1321 cccgggccgt ggtgttgaaa gagggggtgt aaccgtgagg aacccgggcg gtggcaaccg
     1381 gttttcggcc gacgctgctg tgggggccgg ctcgaccagc tccactggtt cggtatgctt
     1441 gaccggctcg actggttcgg catgcttgac cgacgcgcga tccgagcggg gggtggccgg
     1501 cgtgctcgcg gtggccaccg ccatggcgct gctgaccgtc acggcggtcg gtgtgcttgg
     1561 catgacgtac gcggccacgg ctcgatccgt gcgggcggcg gccgatctgg tagcagtgtc
     1621 cggcgcgcaa gccaacggcc agggctctga tgcctgcgac gaggcaaagc gaatcgcagc
     1681 ccgcaatcaa gtggtcttga gcggttgtgc agtcaccggt gacctgatcg actacgtggt
     1741 cgaggtcacg gttcgacgag acgtcggctg gcggctgccc gggttgcccg agcgcgtcag
     1801 cgcgaccgcc tatgccggca gcgtgagcgg tgtgccgtga tgcaccaaca cctgcagcac
     1861 ggcgattgcg gcgcgacggt cgagcgggtg attgcccgag ccgcaggccg gcgagacgac
     1921 gcaacgtggg cagccctgct cgcactcgca ggcgcgcagc aggtccagcg tcgcggccag
     1981 ccaccgctcg gccacccggt acgcctcgtc ggcgaagccg gagcccccgg gcaggccgtc
     2041 gtgcacgaag atcgtgggtg caccggtatc ggggtgaatg acggtcgaca ggccgccgac
     2101 atcacccttg tcgcaggggg cgaaaccggg cagcaggccg atggcggcat gttcggcggc
     2161 gtgcagaccg gccgcgagcg cccgatcgtc caagccgtgc agggcgcggt cgcgctcgtg
     2221 cagggtgaac caggtcgcgc gtgtgcgaaa cgttcgggcg ggcaggtcga ggggtgtcga
     2281 atcccacacc gagccggtga cagcatcgcg ccgcaggtag ccggtgacct gcgaggtcag
     2341 ctcgatggtg ccggcgaaca ggctgccgct gccgatctcg cgccggcgat cggtgccgac
     2401 cacacgcacg tcggtgctgc cctgggcctg ggtgaaccat tcggcacgct gttgcctgca
     2461 caaggcaacg ccggcgcgca ggtcatagct ctccaccagc cactgctcgc cctggtgcag
     2521 gtagaccgca ccctcgtgca cggttcgatc ggcggcagac gcgtccacct ggccgatcac
     2581 tcgtccggtg cccgactgca cgatctcgat gccggtgccg ccgatgctgc gcagatcgat
     2641 cgcatcgaca gctcggtgct gcgaggtcca gtaccaggcc gagccacggc gacgaagcac
     2701 gcctcgcgtg gtcaagtgct caagcagggg cggtatgccg ggcccgaaga actgctggtc
     2761 gtcggcagtg acggcgagtt cctgggcggc ggcggccagg tgggcggcca gcacggcggg
     2821 ctggcccggg tgcagcaccg tggtctcgac gggggtgtcg aagagtgcct ccgggtgatc
     2881 gcacaaccag gtgtccagtg ggtcgggtcg ggcgatcagc acggccagcg cgtcccgtcc
     2941 gtcacgtccg gcgcggccgg cctgctgcca cagcgccgcc agcgaaccgg gaaaaccgat
     3001 ggacaggacg gcgtccatgc cgctgatgtc gacaccgagt tcgagtgcgt tggtgcaggc
     3061 gacgccgcgc aggcgcccgc acgacagggc cgcttcgagt gcgcgacgat cggcggcgag
     3121 gtagccggcg cggtacgagg cgatgccatc ggggtcgatg ctgtgccggc gtgcctgttc
     3181 ggccaccagt tcggcctgca cccgcgaggt ggtgaaggcg agcacctggc caccggtgtc
     3241 gaccaaccgg gtcagcatgc cggccgcctc acgatgcgca tcgccatcgc cctgccagat
     3301 cacgaagtcg agggccgggc gcgtgctggt gtcggtttcg acggccagca ccggatcgac
     3361 gccggcgagc ctttgcaggt gctgggcggg cgcagcgatg gtgcccgacg cgctgatgaa
     3421 caccgggtcg gcgccatagt gtcggcacag tcgccgcagg cggcgcaaca ccgaggccac
     3481 gtgggcgccg aagacacccc ggtagcggtg cgcctcgtcg ataaccacgt accgcagaga
     3541 cctcaacagg ccctgccaac gctcgtggtt gggcagcacc gaccggtgca gcatgtcggg
     3601 attggtgagt acgaaggtgg cgaactcgcg ggcgaagcgg cgctcttcgg cctccgaatc
     3661 gccgtcaagg gtggtgagtc gccaggcgga gccaccgaac tcgcgcgccc gccgggcctg
     3721 gtcgtgggcc agagccttgg tgggcgccag gtagagcgcg gtgtgtgccc gcgcggctgt
     3781 cgcccagcgg tgcggcgcca cgccgggccg aagcgtgcct tctgcggtgg cggcgtgaat
     3841 gggcagcagg tacgccaacg acttgccgga cgccgtcgac gtgctgatgg ccacgtgtcg
     3901 accgtcgaag gccgcctgcg cggcgacctg ctggtgttgc cacaactcgg tgatgccctg
     3961 ctcggccaat gtctgccgcg cgcgagccga cacccaatcg ggcaggtcgg ccgtgacccc
     4021 tgatcgcgcc gggcggtgat gcacgtgacg aacctgcggt tcgttcgaga tccagtccgg
     4081 cacggccatc cccacaggtt gccacgccgc gcggacagaa tcgcgcgccg cggcgacgcg
     4141 agtgcggtct gccgcgggtc gacgtgcgcg acgggtacgc gtgcaatcgc tggactgtgg
     4201 accgggtcgc cgcgggtgac aaggtggccg gcgacggact cgcgggctgc gacctgggtc
     4261 gtggtgagcg accggtgtct gcgaatgggg gttgccgatg tagagggtcg cggtgtgaag
     4321 gaggatcggg gtgctcactc gaagagatcg gtgtcaagga agtgctgcaa tgcgcggcgt
     4381 actcgtcgtg gcccggcttg ccccgggatc tcgacccacc taaagatccc ggcgttcgcc
     4441 gggatgacgg ggctcaggat tgcggcgtgc gccggggtga cgacgggaca cgtcattgag
     4501 taccggggtc acgcctgcgc gatcgggttc acagtgtgcg gtgggtgatc cgtccggcgc
     4561 agaccgtcag cgtggagcgg gtggtgcaca ggccgccggc ggccgcacgc gggtcgtcgg
     4621 cgacggcgag cgggtcacga tccagcaaca cgagatcacc gggggcgccg ggggtcaggc
     4681 ggcgtccgtc caccgaggcg gccatggcct cacggggggt gatcgactgt tccgggtgcc
     4741 acgccggccg ctcgtcgccg ctgcgatgaa ccgcggtcgc catcgcgagc cacgggtcga
     4801 gcggtgcgac cggagcgtcc gaaccaagac gcaggtcgac tccggctcgc agcaggtccc
     4861 gcaccgcgaa ggccatcgaa ccccgctcgc cccagactcg atcggtcagg tcgcgatcat
     4921 cgagcaggtg cgcgggctgc acgctggcgc gcacgcccag ggtggccagc cggcgcgcgt
     4981 ccgccctact gatcaactgg gcgtgctcga tcgaaccgcg tgcaccggtg gcggcaaagg
     5041 catcgatggc tgcgccgacc gcacgatcgc cgatggcgtg cagggccacc tcgagcccgc
     5101 actcccgtgc ctgcgccagc aggccgacca ggtcggcggc cgagtagttt gcctgcccgc
     5161 acgggtgatc ggggctggcc gcgccatcgg tgtagggctc gtgggtccag gcggtgcgac
     5221 tgcccagcga cccatccgag atgatcttga gtggccccat ggtgacgagg ccgcggtcga
     5281 caggcagccg gtcgggggtg ctgggctgtc ggccggcgac agcgccggta cgtgcagtcg
     5341 cggaatgcgg gctgcctgca ccccgtgcga cgctgcgctg cccaggtacc ggcagcgcgt
     5401 ctccggtgcg cagcccggcg tcgatcacgc cacgcagctg gtgcggatac accccggccc
     5461 gcacccgcac ggaatcggcc ccggcggcga tccggaccgg ccagtcgacg aatgagttct
     5521 cgaactcgaa atccaccagg ccggtcactc cgagcgccgc caaccccgcc aggggcgcca
     5581 cgaaatctgc aggcgtgggc gcggctccgg gcaactcgtc cagccggcgt agcaactcga
     5641 accactcgtt ctcgctgacc ggtccgatgc ggggtgctgc gcccaacagc tgcagggccc
     5701 tggagttgag ccagccggtg tgcgcgtcgc ctgagaccag cgcgacggcc cgtccgtcgc
     5761 tcacggcatc gagttccgcg acggttcccg gacgcggcca gcctgccgcg cgataaccga
     5821 agccgagcac cacgcgaccc gagggcacgg acggcgccgc ggctgcgact acggccaaca
     5881 cgtcggcggc acaaccggcc gcgcccagcg gcagcatgcg tgccgtgcgc acccattggc
     5941 ccgcatgcac gtgcgcgtcc cataggccgg gaatggccca ccggcccgcg gcatcgatca
     6001 cctcgacccc ggacggggcc cgtacctcca cgccgacccg gtcgatcacg ccgtccacga
     6061 tcaacacgtc caccggtgcc ccagaaccca cgtccggtcg cgccccagac gccgccgaac
     6121 cggcccgggc accgtcaaga tcgaccaggc gcgctcgacg caccagcaca gacccgctca
     6181 ctcgtgaaag cgtagaagaa tgctcgaccg cacggacgca cgacgactcg ccgacgcgct
     6241 gcgcgcggcc ggctacacct acgacgaggt cgccgccagg ctcggcaccg aagcgctgga
     6301 cgggctcgcc cgcaacgcca ccctgccagc cgatcgcgcc ctcgccggtg cggacgatcc
     6361 gcaggccctg ctgatccgat gcttcgccct gcaacaaccc gtgcccaccg ccggcctgcg
     6421 cgccgcgatc ggcgagatcg tcgaccggcc tgagttggtg cgtccagccg acaccacccg
     6481 cgtcgaccgg cccacgctcg tccgtgccgc cgacaccacc gcccgaaccg ggccgcacgg
     6541 cagcagcgag gccgcgatcg agatccgtcc ctacgccttc gatgacgccg acggcgagca
     6601 tgccggctgg ctggccagcg acctcacccc gattctggac ggacgcgtgg cccggccccg
     6661 cgccgacttc gtgctcggcc tgtcgccggc cagcgtcacc ctggcccagc tcaccatgcg
     6721 caccccggtc gcccgcgctc tcgacctggg caccggttgc ggcgtccaat cggtgcatct
     6781 ggccacccat gccgccgcag tggtcgccac cgacctgaac ccgcgggcct gcgccatgac
     6841 cagactcacc gccgatctca acgcagtcga cctcgatgtg cggcaggggt cgctgtacga
     6901 acccgtcgcc gaggacacct tcgacctgat cgtcaccaac cctccctacg tgatgtcacc
     6961 gccgcagggc gagcggctcg tctatcgcga gggcacccac accgcggacg gactgatgca
     7021 cgccgtggtc accggcgcgc ccgcgcggct caaccccggc ggcgcgctgc aggtgctcgg
     7081 caactgggcg atcaccgccg accaaccctg gcaggaccgg ttggccggct ggctcgaacc
     7141 caccggctgc gacgcgctgg tgctgcagcg cgaacgcctc gacccctatg agtacatcga
     7201 gctctggctc gccgacgcgg gcctggtcgg cacccccgac tacgcccgtc gctacgccga
     7261 atgggccggc tacttcgacg ccctcggcat catcggggtg ggcctgggct ggttggcgct
     7321 ctaccgcaac gagcgcgacc agccgcacct gaccattcag gactggccgc atcaggtgca
     7381 ccagccggtg gccggtgcct tcgcggagtt cacgggcgcc gtcaccgccg cccgccgaag
     7441 cgacgccgag ctgctcgccg gcaccttaac cacggccccc gacctgaccc aggagaccct
     7501 cggacgcccc ggcgccgccg atcccgaaca catcgtgctg cgccgcaccg ccggcttcgg
     7561 acgtgccgtc gaggtcgacg ccgcgctggc cgccgtgatc ggcgcctgtg acggcgacct
     7621 gccgctggga atgctgatcg aggcggtcgg cggcctgctc gaggtgaacg ccgccgacct
     7681 cgtcgccgag ctgctgccgc ggctgcgcac gctgatcgcc gacggctacc tgcgcggctg
     7741 atccactcgc catctggccc gcgctggtcg tccgacccgg ctttgtggct accattcccc
     7801 ccgagaggcc gtcccggccc cgacccccgc agtaggaaga atctcgtggc aacagcaggc
     7861 aagaaggcac tggtgatcgt cgagtcaccg gccaaggcga cgaagatcgc cagttttctc
     7921 ggcaacagct acgtcgtgga atcgagccgt ggccacgtcg ccgacctgcc caccggggcg
     7981 tccgaggtgc cggcgaagta caagggcgag aagtgggcgc gcaccggcgt caacgtcgac
     8041 aacaacttcg agccgctgta cgtcgtgcag gccgacaaga agggcacgat ccgcgagctg
     8101 aaggccaagc tcgccaccgc ggacgaactg ctgctggcca ccgacgaaga ccgcgagggc
     8161 gaggccatcg cgtggcacct gctgcagcag ctcaagccca aggtgccggt gcggcgcatg
     8221 gtgtttcacg agatcacccc gcaggcgatc gccgaggcgg tcgccaaccc gcgcgagctc
     8281 gacaccgacc tggtcgatgc gcaagagacc cggcgcatcc tcgaccggct gtacggctac
     8341 gaggtcagcc cggtgctgtg gaagaaggtg atgcccaagc tgtccgcggg ccgggtgcag
     8401 tcggtggcga cccgcctggt ggtcgatcgt gagcgcgaac gcatcgcgtt ccggtcggcc
     8461 ggctacgccg gcatcgaggc cgtgctgaat gccggcgatc aggccgagcc gcccaccttc
     8521 aaagccaccc tgcagcaggt cagcgacctg cgggtcgccg ccggccgcga cttcgacgcc
     8581 cagggcaagc tgaccggcaa gggtctgctg cacctgtccg ccgagcaggc caagagcctg
     8641 gccgaagtcc tcgagtcggc ctcctacgcc gtcgcctccg tcgaggccaa gccgtacacc
     8701 cgtcgtccgt acgcaccgtt ccgcaccacc accctgcagc aggacggcgg gcgcaagctc
     8761 ggcttcaccg ccgaacgcac catgcgggtg gcgcaggacc tgtacgagcg cggcttcatc
     8821 acctatatgc gtaccgactc ggtgaccctg tcggccagcg cgatcaccgc ggcccgcgcc
     8881 caggtcaagc agctgttcgg cggccagtac ctgcccgatc agccccgcgt ctacacctcg
     8941 aaggtgaaga acgcgcaaga ggcgcacgag gcgatccgtc cggccggtga gcacttcgca
     9001 gccccggccg agaccgggtt gagcggcgac gccttccggc tgtacgagct gatctggaag
     9061 cgcacgctgg cctcgcagat gaaggacgcc gtcggcgaga ccgtcacggt gcgcatcggc
     9121 gccacactgc cgcagcgact gtcgatcacc gacgccacca ccggtgagat caccgaaacc
     9181 gggcgctgca cgttcggtgc gtccggtcgc accatcacgt ttccgggctt tctgaaggcc
     9241 tacgtggtcg gggtcgacga cgacgagacc tcggacgacg cgcaggcccg gttgccgcag
     9301 ctggtggccg gggccccgtt gagcgccgac gaggtgaagg cggtcagcca cgacacccgg
     9361 ccccccgccc gctacaccga gccgtcgctg gtggcgaagc tcgaagaact cgagatcggc
     9421 cggccgtcca cctacgcctc gatcattcgc accatcacca gccgcgacta cgtgttcaag
     9481 aagggctcgg cgctggtgcc cacctggttg gccttcgcgg tcacccggct gctcgaggag
     9541 cactttccca agctggtcga ctacacgttc accgctgccc tcgaagagac cctggacgac
     9601 atcgcgaacg gccaggcggc ccggctgcag gtgctcagcg acttctactt cggcccggaa
     9661 gggtcggccc acgagggtct caccaccctg gtcaacgaac tgggcgagat cgatgctcgc
     9721 gagctgtcca cgcacccgct gggcgattcg ggcatcaacg tgcgcgtcgg ccggtacggc
     9781 acctacgtcg aggatcccga cggccggcga gcgaacgtgc ccgatgacct gccgccggac
     9841 gaactgactg ctgagcgtgc gcgcgagttg ctggagcagc cgatgggcga ggagcgcgag
     9901 ctgggcgtcg accccgccag cggtcacacc gtcgttgcca agaacggacg ctacggaccc
     9961 tacgtcaccg aaaccctgcc cgaggacgca cccaagtcgg ccaagccgcg caccggttcg
    10021 ctgttcgcgt ccatgaccct cgacacggtc acgctggacg acgcgctgaa gctgatgagc
    10081 ctgccccggg tcgtcggcgt cagcaccgac ggggtcgaga tcaccgcgca gaacggacgc
    10141 tacggcccct acctgaagaa gggcaccgac tcccgttcgc tgaccagcga ggagcagatc
    10201 ttcggcatca cgctggacga ggccgaggcc atctacgccc agcccaagac ccgtggcgcc
    10261 cgcaccgcgg ccgcaccgct gaaagaactc ggcgccgacc cggtcagcgg caagccgatg
    10321 gtgatcaaag acggacgctt cggcgcctac gtcaccgacg gtgaggtgaa cgcgaccctg
    10381 cgcaagagcg attcgatcga ggccatcacc gccgagcgcg ccgccgaact gctggccgag
    10441 aagcgcgcca agggccccgc ccccaagcgc accacccgcc gggcgcccgc caagcgtgcg
    10501 accggtgcgc aggcgtccgg caccaaggcg gctggcacca aggcgaccgg cgccaaggcg
    10561 tccggcacgg cgaccaagcg ggcagcgaac accaacagcg ccacgaagcg gggcggctag
    10621 ccaccatgac cgacctcaac gatgaggtgc tacgcgtcga cctgccacct gcggcagccg
    10681 tcgcgaatct gacgggtccg gccaccggca ttcatcggct ggtggcgcgt ccgccggtgc
    10741 agaccacggc catcgtgatc gacaccgacg atcaccggtt gctcgactgg tcgatcgagc
    10801 tgtcccggat cactgaaacc gggcagtggg tgctccgggc gccgttgtgg gcgcctccgt
    10861 tgcaggccga gacgatctcg ccacaggccg aggacgacct gcccgtcgac ctggccgacg
    10921 tgctggtgcc gttccgccgc ggcggcatcc tgggcccccg gctgagcgtc gagacgcaac
    10981 ggcgccagtt cactctcgag ggctcggacg gggcggtgat cggcgagttg agcgacgagc
    11041 gtcaccgcat cgggagtcat gccggccaga tgacggcata ccgcaacgtc accctgaagc
    11101 tcgcccgccc gcgacccgag cacctgcagg cgatcacggc cgccttcgtg gccgccggag
    11161 gcgtcgtggt caccgggttc gaacccctgg ccgtccggct gggcctgtcg caccacggtc
    11221 gcggacgcag cgcgctttcg gcgaaggtgc cgatcgggga ctacgtacgg gcccagttcg
    11281 aatcgcgctg gcgccgcctg ttgcgcgccg atctcgaggc acgcaccggc tcgggcgacg
    11341 acggtgccct gcgcgagaac gtcgctgccc tgctgcagga gttggtgggg ctcgaaccgc
    11401 tgctcgatgc cgactgggct gagcaggcga aacagcacgc cgaggcggcg tccgagacga
    11461 cgcgcccgct gcagcacacc cagcgctggc tgcgcattct cgacctgctc gcgacggcca
    11521 gcacgtcacc gccggtgcgg gtggcggcgg agcggctgac cggtccggtg ctcgcccagg
    11581 aactcgaggc cgtggtgcag accctgctcg accagtgccg caccctggag ccctattcag
    11641 aggacgcgcg ctggtcgcgc gcccaccagc tggcggggcg tgcggtgacg ctcagcatgc
    11701 tggcgcgcga cgtgttcggc aagccggcca agcagctgcg caagcacctg agcccgatca
    11761 gcgaggccct ggcacacacg ctgcgtccgg acccggtcgc gctcaagcgt gacctgcatg
    11821 gcctcagcac cgctgaaatc ttcgcggccg gacgagccta cgagcgcacc gtgctctcgg
    11881 tggactacgc gcgcgagagc ttcgtccgcg aatggccgca actgggcgag gcgctgcgtc
    11941 cgcacgtgat tcggcccagg gctccgcaca ccccggcgcg tccgggtgct gtggcggcac
    12001 gtcctgctgc gccgggccgg gcggtcgagc cggcctcgga cgcctccgtg ccgatacctg
    12061 cagccacgtc ggcggcggtg ccctcgtgag cggcgtcttc gtcgtcttcg agggtgggga
    12121 cggagtcggc aagtcaaccc agaccaagct gctggccgaa tgggccattg cgcagggacg
    12181 caccgtccgg gtcaccttcg agcccggcga caccgcggtc ggacgcagcg tccgctccat
    12241 cgtgctcgac cccgccaccg gtgacctgga cgcccgcgcc gaggctctgc tgtatgccgc
    12301 tgacaaggcc caacacgtgc atgaggtggt gcgtccggcg ttgcggcggg gtgagctggt
    12361 ggtctgcgac cgttacatcg actcgatgct ggcctatcag ggcgcgggcc gggtgctcga
    12421 cctcgacgag gtggccggca tcgccgaatg ggcaacggac ggcctggtgc ccgacctgac
    12481 cgtgctgctc gacctcgacc cggccgaagc ggtcggggcg atcgagcagc ccgaccggct
    12541 cgaacaggcc ggcgccgacc tgcatcagcg ggcgcggcgg ttcttcctcg aactcgcgag
    12601 ccgcgagccc gagcgctatc tggtgctgcc cgcccgcgaa ccgcgagatc gcatcgccgc
    12661 gcaggtgcag caacgcgtcg gtgagttgtt gtcgtcggcg gctggcagaa tcgccacatg
    12721 agcgtgtggg ccgacctggt cgggcagcag cgggtggtcg acgtgctgcg ccgggccgtc
    12781 gcgggtggcc cgcacgccat gacccacgcc tggctgatca ccgggccgcc gggctcgggg
    12841 cgctccaatg ccgcgcgctc gttcgcggcc gcgctgcagt gcgccgaggg cggctgcggg
    12901 gtctgttcct cgtgtcgcac cgtcttgtcg ggggcgcatc ccgacgtcac gctggtgcgc
    12961 accgagcagc tgtcgatcgg cgtcgacgag gtgcgcgagc tcgtccgacg ggccgccatg
    13021 tcgcccacca tcgggcgcta ccaggtgctg gtggtcgaag acgccgaccg ggtgaccgaa
    13081 cgcggcgcgg acgcgctgct caaaagcatc gaagagccgg cgccccgcac ggtctggctg
    13141 ctctgcgcac ccacctcgga cgatgtggtg gtcaccatcc gctcgcgctg ccgcgccgtg
    13201 cacctcgcca cgcccagcaa cgccgctgtg accgagttgc tggttcggcg cgacggtgtg
    13261 ccgcgcgaac tcgcggcctt cgccgcacgg gtcgcgcagg gccacatcgg ccgggcccgc
    13321 atgctggccc ggcacgaggc ggtgcgcatt cgccgctccg acctgctcaa gctgccggct
    13381 cggctggtgt cgttgcccga ctgcctgacc gcagccgcca acctggtcga ggcggcggcc
    13441 gacgaggccg cggccaccac ggccgatctg gacgcccgcg agaaggccga gctgtccgag
    13501 gcgctcgggt tcggcaccaa gggtgcccgg ccgcgcaacg cgcaggcggc gcttcgcgag
    13561 ctggacgatc agcagaaggc gcgggccaaa ccgctgcagc gcgatgccct cgaccgggta
    13621 ttgaccgagc tgaccacgta ctaccgcgac gtgctgtgcg tgcagaccgg tgtcggcgcc
    13681 gaactgatca acgccgaggt gcggcccgag atcgaggcga tggcccggca gcatgcgccc
    13741 gagcgcaccc tgcggcgcat cgacgcgatt ctcgaatgtc gcaccgcgct cgagggcaac
    13801 gtggcgccgc tgttggccat ggagtcgttg atgatctcga tcgccgtctc gatctgagac
    13861 ctgtccataa tcctcgacct atcgatggtc gagcttgacc agacccctgc gaccagggcg
    13921 tgtcgcataa ccaagggcgt tccgcgagga cgtgcccagt gcatctgatc gaaaggcgga
    13981 agacgggtcg atagtgggtc ctatcgctca ggatgacaac gcagcgagcg ggcgtacggg
    14041 gcgcgcggca gcgcggtcag ggttatgcga cacgccgaga tgtctcgacg cactcgggcc
    14101 ccgaatcgcg tgccaatcag cccttcactg ggcccgaggt ggtggtggct gtggaaggtt
    14161 cgccgcttgg cggagcccgt ccgcggtggg cgtccgaacc tgcagtgcaa tcgccgtgcg
    14221 gcgagtgtcg ctgggcggcc cggtgatgct cggcaggcac gatggaggtc agccctgcgg
    14281 aggagaacca tgtcccacac cgtcgacgac gagcagacct cgccgtattc gcccgaggct
    14341 gcggcgggcg aggcccgcac acccacgctg ccgcaaccgc cccgattcga tgatctgacg
    14401 cccgaagagg cgaaccgggt gcggttgatc agcgagcagt tcgcgcccaa gctgagcgac
    14461 ttgcaggagg gccaggacgc cccgcaggcc gcgtccgtcc ggccggatca gcacgcgcag
    14521 caggccgcgt ccgcctggcc ggttcagcac gcgcagcagg ccgcgtccgt ccagtccggt
    14581 caggacgccc cgcaagccgc gtccgcctgg ccggatcagg ccgcgcagca ggccgcgtcc
    14641 gcctggccgg atcaggccgc gcagcaggcc ccgtccgccc agccgcagct ctatgcaccg
    14701 caggtcgcgt caccgcagac cgggcaggcc gatcagccca cgcgccagca ggcggtcgtc
    14761 gggcaacctg ctccggctcc gcaacaggac tagcaggtga accgtccgca accagcggtc
    14821 gaggcggcgg cgcgtccgca gcaacccgtg gacaaccagg cgacacgtcc acaaccagcg
    14881 gtcgagcagg ccgctgcggc tgccggggtg aacgagcagg ccacccgtcc gcaacccgcc
    14941 gtcgtggcac atcagaacga ctcgatcttt cgggcacaga gcgtcgcgac gccgtccgat
    15001 cccccctcgg ccgaacagca gaagctcgac gccgagcgtg ccgcccgccg cgaggcccgc
    15061 gagcaggccc tggcacagcc cgatgtgcgc acggtggccg ctcccgagcc gatcgtggtc
    15121 acccgacgca ccacggaccg gttcctgggg tctttcgcct tgttcgtgct gcgcctggtg
    15181 gtggccgcga tcctggctgt tcgggggctt ggcatcgtta ccgacctcac ctcggccgag
    15241 gcgttgttcg ccaccacgat cctgcccgag ccgcggatcg cggccattgt gaccggctct
    15301 gccgccctcg ccatcgcggt cgcgttggtg ttcggcctgc tgacccgcgt ggcgggcttg
    15361 ggtgtgatgc tgatcgccgg tggtgcgctg gcgttcgtgc tgtggggcaa ctggagcccg
    15421 ttcgtggccg gacgcgccgg cttcatcggc gagctggagc tgttgctggc ggccgtcggt
    15481 ctggtgtttc tctgcgtcgg cgcgggcgga ttcagcctgg atcggagctt ccgtaagagc
    15541 cgcgagaagg accgtccggc cgatcaggca ctgtgagagc cacctcggtg cagcttggcg
    15601 cccgccgcta gattgtcgtg gtgcgcactc tcttccgctg ggcggccgcg gctgtcggtc
    15661 tgaccatgct ggcgggctgt acgccgtacc tgccaacctc gccgaccgcg tcgtcctcgc
    15721 cggcgaccag cgcaccggcc accggggcgg cttcgccttc ggcgactgct ccggccggcg
    15781 cggtgatcac ggtgtcgagc gtgccgggcc tcgagatcga ccggccacga ctcgccgacc
    15841 cctccgtcga accggagggt ttcgcggacg ctccggcggg cgagggcctg gcgggctacc
    15901 tcgaccagcg cgtccggtgg agcacctgcg cgcaggagta tcagtgcgcc cagatcctgg
    15961 ctccgctcga ctacgccaac ccgtccgagc aggcgatcac gctggcgctg gccaagaagc
    16021 cggccacgaa gtcgcccagg ctcggcaccc tgttcgtcaa tccgggcggc ccgggcgcgt
    16081 ccggacgcaa cctggtcacc tacttcgcct cggccgggct cgagcagtac gacatcgtcg
    16141 gctgggatcc ccgcggcacg aacgcgtcca cgcccgtgca gtgcttcgac gacgagcaga
    16201 ccgaggcttt caccaggctc gactcgtcgc ccgatgacgc ggccgagacc gaggcgttga
    16261 tcgtcggcac ctacgacttc gcgcagtcct gctggcagca cagcggacgg ctgttgcagc
    16321 acatctcgac cgtcgacacg gtgcgtgatc tcgacctgat gcgcgaactg ctcggtgaca
    16381 agctgctgag ctacgtgggg tactcctacg gcacccagat cggctcgtac tacgccgacc
    16441 tgttcccgca gcgggtcggc cggctggtgc tcgacgcggc ggtcgacatc accgacgacg
    16501 actccgtgat ccaggccatg ggtttcgatt tggccctggg caacttcgcc acctggtgcg
    16561 cccggcaggg ttgcacgctc ggcacgtcca aggacgcggt gctgaagtcg atcaccgact
    16621 ggctcgaccg gctggattcg gcgccggttg aggtggggga ccgcacgctc acccagagcc
    16681 tggccgccgc cggtgtggcc tcgtacctgt acgggggcgt gccggcctgg aagtcgctga
    16741 gtgccgatct cgaggctgcg atggacggcc ggggcgccaa cctgctcaag gcggcggact
    16801 cgctcaacga ccgtaaccag gacggcagct tcgggtcgct gttctactcg ttccccgcga
    16861 tcggctgcgc cgacggctac gacaagggcg tgaccgaggc cgagcagcgc tgggtggccg
    16921 accagaagaa ggcgccgatc ttcggcaagt acttcggccc cgggtacacc tgtccgctgt
    16981 ggccggtgca cagcacgatc cagttcaagc cgaccggctc aggtgccgcg ccgatcgtcg
    17041 tggtgggtgc gaccggtgac ccggccaccc cgtaccagaa cgccgtcacc atggccaagc
    17101 aactgaagtc gggggtgctg gtcacctacg aaggcgaggg acacggcacg ttcggcggca
    17161 agtcgacgtg cgtggacgct ctggtgatcg actacctgac caagggcacg gtgcccaccg
    17221 acggggtgag gtgccagtag gcgtgggccc tcgaatcgcc tcggcctcgg ctgctgccct
    17281 atagttggac ggcgccggtg cgtccggcag gccgccttag ctcagttggc agagcgattc
    17341 act
//
LOCUS       DAOSUG010000002        17062 bp    DNA     linear   ENV 10-JUN-2024
DEFINITION  MAG TPA_asm: Micropruina sp. isolate AMR_MDS_2195
            NODE_2083_length_17062_cov_4.389134, whole genome shotgun sequence.
ACCESSION   DAOSUG010000002 DAOSUG010000000
VERSION     DAOSUG010000002.1
DBLINK      BioProject: PRJNA925477
            BioSample: SAMN32864262
            Sequence Read Archive: SRR4236673
KEYWORDS    WGS; ENV; Metagenome Assembled Genome; MAG; Third Party Data; TPA;
            TPA:assembly.
SOURCE      Micropruina sp. (wastewater metagenome)
  ORGANISM  Micropruina sp.
            Bacteria; Bacillati; Actinomycetota; Actinomycetes;
            Propionibacteriales; Nocardioidaceae; Micropruina.
REFERENCE   1  (bases 1 to 17062)
  AUTHORS   Abdulkadir,N., Saraiva,J.P., Zhang,J., Stolte,S., Gillor,O.,
            Harms,H. and Rocha,U.
  TITLE     Genome-centric analyses of 165 metagenomes show that mobile genetic
            elements are crucial for the transmission of antimicrobial
            resistance genes to pathogens in activated sludge and wastewater
  JOURNAL   Microbiol Spectr 12 (3), e0291823 (2024)
   PUBMED   38289113
REFERENCE   2  (bases 1 to 17062)
  AUTHORS   Abdulkadir,N.
  TITLE     Direct Submission
  JOURNAL   Submitted (24-MAR-2023) Environmental Microbiology, Helmholtz
            Center for Environmental Research -UFZ, Permoserstr 15, Leipzig
            04318, Germany
COMMENT     The annotation was added by the NCBI Prokaryotic Genome Annotation
            Pipeline (PGAP). Information about PGAP can be found here:
            https://www.ncbi.nlm.nih.gov/genome/annotation_prok/
            
            ##Genome-Assembly-Data-START##
            Assembly Method        :: SPAdes v. 3.11.1
            Genome Representation  :: Full
            Expected Final Version :: Yes
            Genome Coverage        :: Not Applicable
            Sequencing Technology  :: Illumina MiSeq
            ##Genome-Assembly-Data-END##
            
            ##Genome-Annotation-Data-START##
            Annotation Provider               :: NCBI
            Annotation Date                   :: 05/04/2023 04:57:10
            Annotation Pipeline               :: NCBI Prokaryotic Genome
                                                 Annotation Pipeline (PGAP)
            Annotation Method                 :: Best-placed reference protein
                                                 set; GeneMarkS-2+
            Annotation Software revision      :: 6.5
            Features Annotated                :: Gene; CDS; rRNA; tRNA; ncRNA
            Genes (total)                     :: 2,776
            CDSs (total)                      :: 2,750
            Genes (coding)                    :: 2,714
            CDSs (with protein)               :: 2,714
            Genes (RNA)                       :: 26
            tRNAs                             :: 24
            ncRNAs                            :: 2
            Pseudo Genes (total)              :: 36
            CDSs (without protein)            :: 36
            Pseudo Genes (ambiguous residues) :: 0 of 36
            Pseudo Genes (frameshifted)       :: 2 of 36
            Pseudo Genes (incomplete)         :: 32 of 36
            Pseudo Genes (internal stop)      :: 2 of 36
            ##Genome-Annotation-Data-END##
FEATURES             Location/Qualifiers
     source          1..17062
                     /organism="Micropruina sp."
                     /mol_type="genomic DNA"
                     /submitter_seqid="NODE_2083_length_17062_cov_4.389134"
                     /isolate="AMR_MDS_2195"
                     /isolation_source="Activated sludge of municipal
                     wastewater treatment plants"
                     /db_xref="taxon:2737536"
                     /environmental_sample
                     /geo_loc_name="Canada"
                     /lat_lon="49.25 N 123.1 W"
                     /collection_date="2015-06-16"
                     /metagenome_source="wastewater metagenome"
                     /note="metagenomic"
     gene            <1..583
                     /locus_tag="PLF56_00075"
     CDS             <1..583
                     /locus_tag="PLF56_00075"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_002514581.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=2
                     /transl_table=11
                     /product="ACT domain-containing protein"
                     /protein_id="HOQ51989.1"
                     /translation="SSFSTKPGTWVKDIEVKEGTAMEQPLITGVAHDRSESKITVVGV
                     PDRVGAAAEVFTAVAEAGVNIDMIVQNQSIANDNTAITFTLPGGDKLTALDALHGARE
                     RIGFVDTLYDDAIGKVSVIGVGMRSHPGVSATFFRALAEAGINIQMISTSEIRISVVV
                     ALTDVDAAVRAAHTAFGLDSVTGEAVVYAGTGR"
     gene            complement(1208..2251)
                     /locus_tag="PLF56_00080"
     CDS             complement(1208..2251)
                     /locus_tag="PLF56_00080"
                     /inference="COORDINATES: ab initio
                     prediction:GeneMarkS-2+"
                     /note="Derived by automated computational analysis using
                     gene prediction method: GeneMarkS-2+."
                     /codon_start=1
                     /transl_table=11
                     /product="hypothetical protein"
                     /protein_id="HOQ51990.1"
                     /translation="MATKHRKHKRDTLALVESPAQLLNVIEWAHHHGAEKQTRLALLP
                     PREATTRLQLEAMADLARDAGLAATWYEPRRSAASLVAVAWELFPRLAKASRLVVGDP
                     FSRFVQAVLPLADVAEVVVVDDGTATISFISQLTRGERLARWHLAETGRDVVVDLLSD
                     HARQFFSPSTRDERSIEVFTAMPVQAPEGITQTANTFAWTRGRFGPPKIKDAVDVIGT
                     SLVETGVVNEERYLVGVQAVTERFGAGRYFAHRRESEAKLARIATATKLKVVHPDVPL
                     EIAVRRGPVGRLMVSYPSTVMHTLPMVLVDTGVELAVADVPSGWLGAGAPSGAGDFLQ
                     TVTSTARERHGLT"
     gene            complement(2259..3176)
                     /locus_tag="PLF56_00085"
     CDS             complement(2259..3176)
                     /locus_tag="PLF56_00085"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_013863731.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_process: GO:0016051 - carbohydrate biosynthetic process
                     [Evidence IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="N-acetylneuraminate synthase family protein"
                     /protein_id="HOQ51991.1"
                     /translation="MIHTTRERLIGDRPVGPNHPVYVTAEIGLNHNGDVATCLALIDV
                     ALEAGADAVKFQKRTPKICTPRDQWDVERDTPWGRMTYIDYRHRIEFGVDEYRVIDEY
                     CRDKGIQWFASPWDVPSVEFLNGFDVPAHKIASACLTDDELLRACRAYGKTVILSTGM
                     STPSQIRHAVEVLGSSNVVLVNATSTYPAENSELNLRMINTLGTEYPNVPIGYSGHER
                     GLQTTVAAAALGACFIERHITLDRTMWGSDQAASLEPGGLSRLVRDVRVIEEALGDGV
                     KRVYEGEYAAMRKLRRVPGVVADAATQVA"
     gene            complement(3173..4342)
                     /locus_tag="PLF56_00090"
     CDS             complement(3173..4342)
                     /locus_tag="PLF56_00090"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_013863872.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="acylneuraminate cytidylyltransferase"
                     /protein_id="HOQ51992.1"
                     /translation="MTALAVIPARGGSKGVPGKNLAPVAGVPLVVRAIRAAQASGRVD
                     AVAVSTDDDRIAAIAESAGALVIRRPDELAGDQASSESALLHALDAFERLTGDSVDVL
                     VMLQCTSPFLTPDEVAACVEAVRGGADTAFTAAQSHGFLWRRDAAGNAVGVNHDKATR
                     PRRQDREPEFLETGAVYAMSADGFRQHRHRFFGTTALIETDPARVLEIDETSDLQRAR
                     TLAHLVEVPASVPGRSEIDAVVLDFDGTQTDDSAQVGSDGSEQVRVHRGDGLGIAALR
                     RAGIPVLILSSEVNPVVRARARKLSVPVIHGVDDKGTVLRDWCSTERIDPARVLYAGN
                     DVNDLGCFEVVGWPVAVASAHPVVRDAARLVTSVPGGHGAVREIASWILGKELTS"
     gene            complement(4339..5652)
                     /locus_tag="PLF56_00095"
     CDS             complement(4339..5652)
                     /locus_tag="PLF56_00095"
                     /inference="COORDINATES: ab initio
                     prediction:GeneMarkS-2+"
                     /note="Derived by automated computational analysis using
                     gene prediction method: GeneMarkS-2+."
                     /codon_start=1
                     /transl_table=11
                     /product="hypothetical protein"
                     /protein_id="HOQ51993.1"
                     /translation="MTAVDPSVQTPPIRVLVLADTDSRWKWGFLTAKQLFPAATHTGL
                     LLESASVPSARQLADTGVTVQPDVVGGVPDFLELDDLDEVDALVLALPGGAVQAVLHG
                     LARRWPRAGHRPLVISGYVGVVYEKLAEGLLLRAGSDVILANSATDAEEFRRLLDGTG
                     ADPAVVLETVLPFLGEPDSARPHDGPFTVTFAAQPGVPLTRAERELVLRRLFRHARLH
                     PDREVLLKVRGALGERSTHADYHPYQRLAAKLSTQPPANFRIVQGNMGEVLQRTDLLV
                     TVSSTAAVEAMHRGIPTAILTDFGIKEVLGNHYFIGSGCLAGFADLDDGLLPTVDSGW
                     ARSRGLGASDVVEQARAALAARLPEQVRPPLRPFYSMQNAPGMVPQLLGRYDLDGHGH
                     ALETDDALLADLPHAVRSLVSRAARTAYRQGVDTVAPTLRRLAGI"
     gene            5879..6853
                     /locus_tag="PLF56_00100"
     CDS             5879..6853
                     /locus_tag="PLF56_00100"
                     /EC_number="2.4.-.-"
                     /inference="COORDINATES: protein motif:HMM:NF012745.2"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_function: GO:0016757 - glycosyltransferase activity
                     [Evidence IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="glycosyltransferase family 2 protein"
                     /protein_id="HOQ51994.1"
                     /translation="MKLSVIIPCYNVAEQLPTLVRSLQGNLRRDFEFVLVEDCSADDT
                     PSVLAELIPTVPGARLVQHDRNRGISAARNSGLAVAQGTYLVWLDSDDWIGPGYLDQL
                     VRVIERLGCDFVRTDHVRVLGRRRTIVRSPESRRNLVLPPRTGIAPPFRSTSVDYPNV
                     WAGIQHRRLATQGLLTFDEDLRTCEDRHWIWRMHRDAQSFATVGLLGLFYRQGLTQSL
                     SHISSPDQLHFLDAMQKVVDETLADPTGEPLLLKAVDAYCALINFHLTKRGRLTPALQ
                     RELIRRSILALDGLPQDLLDQALKEGAPRRRTLLTRMRRNPQYLKEVA"
     gene            6850..8205
                     /locus_tag="PLF56_00105"
     CDS             6850..8205
                     /locus_tag="PLF56_00105"
                     /inference="COORDINATES: protein motif:HMM:NF019034.2"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="polysialyltransferase family
                     glycosyltransferase"
                     /protein_id="HOQ51995.1"
                     /translation="MIQLCVASTLYGTATLAAAVDAGLLGTPERRVLVISNNAEAPEA
                     QPGPQAQPGFAELAGRFDAVVSWNDVVYPFHPTQWRPRASDATLWERLVRQAWQLDEG
                     PLELVMESIQAPPALALAGLFLDAPIRLYSDGLMTYGPTRRVLGDQIGSRLMQVLYLE
                     LIPGLEPLVAHEYGPATMAIPHAAMAAVLAEVTAATPATPGLPSGCALIVGQYLSAAE
                     LISEDQERALYVDMVRRAVALGHRDIVFKPHPTAPRHWQQHLSAAASAEGARLSFVTT
                     PALAEVLYERLRPAVVIGCFSTALLTASAFYGLPVGQLGTALLFERLAPFANSNRVPV
                     VLVSALVPDLAAGATPRPVSTDAAGGIDALRRLVATLAYCMQPAICDDLRGIAVDELR
                     TKPSVYRRLISRGRLARLGLPGAPVDLSFLLGNRHVRRGAQGLLAAPKRVRRKLARAL
                     N"
     gene            complement(8461..9120)
                     /locus_tag="PLF56_00110"
     CDS             complement(8461..9120)
                     /locus_tag="PLF56_00110"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_013161708.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="lactate utilization protein C"
                     /protein_id="HOQ51996.1"
                     /translation="MSSKEAILGRIRSALVDVPKVSAADDVEVNWQYGQRPDTAADDM
                     IGLFVERVEDYKAIVSWVVPAELPARIANALADHGATSAVVPAGAPREWHEAVAAAGI
                     AVVTDDPVLSHAELDATGGVVTASRVGIAETGTIVLDHDADQGRRALSLVPDLHVCVI
                     RADQVVSDVPEAVARLQASIDERRALTWISGPSATSDIELNRVEGVHGPRTLHVLLVN
                     E"
     gene            complement(9120..10652)
                     /locus_tag="PLF56_00115"
     CDS             complement(9120..10652)
                     /locus_tag="PLF56_00115"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_014846330.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_function: GO:0009055 - electron transfer activity
                     [Evidence IEA];
                     GO_function: GO:0051536 - iron-sulfur cluster binding
                     [Evidence IEA];
                     GO_process: GO:0019516 - lactate oxidation [Evidence IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="LutB/LldF family L-lactate oxidation iron-sulfur
                     protein"
                     /protein_id="HOQ51997.1"
                     /translation="MSPDRTKTVQDTAVTHERRTPAPPPVTLIGMPKFPKNAKKELAN
                     AQLRSNLRHATTTIRNKRVKVVNELPHWEDLRLAGEAIKNRTLRHLDDYLLQLEASLT
                     ANGAIVHWANDAAEANQIVIDITRSKGADEVVKVKSMATQEIDLNEALEEAGIAAWET
                     DLAELIVQLGHDRPSHILVPAIHRNRSEVREIFLEEMGRYGTPPPEGLTDDPAMLAGA
                     ARVHLRNKFLRAKVAVSGANFAIAETGTLVVVESEGNGRMCLTLPETLISIVGIEKTL
                     PTFADLEVFLQLLPRSSTAERMNPYTSMWSGVQAGDGPQEVHVVLVDNGRTKVLADEV
                     GRQALRCIRCSACLNICPVYERVGGHAYGSVYPGPIGAILTPQLRGTASEVDQSLPYA
                     SSLCGACFEVCPVRINIPEVLVHLRNKVVEEHHGPSAWSIGMKAGGWVMGKGSRFGTL
                     EKGAELFGAVWPGATVGHIPGTGAWTGARDLKKPPTETFRDWWVKNEKAKRKGDAEQK
                     DA"
     gene            complement(10649..11443)
                     /locus_tag="PLF56_00120"
     CDS             complement(10649..11443)
                     /locus_tag="PLF56_00120"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_014846329.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_function: GO:0046872 - metal ion binding [Evidence
                     IEA];
                     GO_function: GO:0051536 - iron-sulfur cluster binding
                     [Evidence IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="(Fe-S)-binding protein"
                     /protein_id="HOQ51998.1"
                     /translation="MSVPTGTVALFATCINDMMFPDTPKAVLRLLERLGCTVDFPLQQ
                     TCCGQMFTNTGYFKQAMPSVRNFVDTFGPYEWVVGPSGSCIGSVRDQHPMLAKRLGDP
                     GLQREVDAIVAKTLDLSEFLVDVLEVTDVGAYFPHTVTYHSTCHSLRVTKVGDRPYKL
                     LRAVKGLTLIELNDSDQCCGFGGTFSVKNPDTSVAMGTDKMRNVLDTGAEYLVAGDNS
                     CLLHVGGLLSRNEAGVKTIHLAEILASTEADLVPAGSATTQAGGVR"
     gene            complement(11596..13032)
                     /locus_tag="PLF56_00125"
     CDS             complement(11596..13032)
                     /locus_tag="PLF56_00125"
                     /EC_number="2.7.1.-"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_013861869.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_function: GO:0005524 - ATP binding [Evidence IEA];
                     GO_function: GO:0019200 - carbohydrate kinase activity
                     [Evidence IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="rhamnulokinase family protein"
                     /protein_id="HOQ51999.1"
                     /translation="MATTQLSVGAVDLGASSGRVMVGHLAHGRVELEQVARFGNGVVE
                     LPDGLHWNLLGLYSEVLTGLHDAAARHPLASVGIDSWAVDYGLLNGGGGLVGTPYAYR
                     DARTARGVEKVHATIAFDALYRRNGLQFLPFNTLYQLAAEPDLSGDGPRDPQRLLLVP
                     DLLAYWLTGRQVAEYTNASTTGLVNARTRQWDRELIARLGYPEGLLPALVQPGVTVGS
                     LRPTLVEEIGQSLDVVSVPSHDTAAAVVGVPMDPTRAAYISCGTWGLVGVELPAPVLT
                     DAARAANFTNEGGVDGTTRFLHNVMGLWVLSETLRVWRSRGGSADLGTLLAAAAEVPA
                     PPHLVDLNDDRFLPPGDMAVRIGEWLDERGLPQPGSDVAIVRLIIESLAEHFATAVRT
                     AAELSGVRVEQIHVVGGGCQNQLLCQLTADRSGLAVVAGPIEATALGNVLVQARAHGR
                     VGSLDEIRQIIARSFDPIRYAPRHRAAD"
     gene            complement(13037..15073)
                     /locus_tag="PLF56_00130"
     CDS             complement(13037..15073)
                     /locus_tag="PLF56_00130"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_020574267.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_function: GO:0016491 - oxidoreductase activity
                     [Evidence IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="bifunctional aldolase/short-chain dehydrogenase"
                     /protein_id="HOQ52000.1"
                     /translation="MANVAVDQLISRSNRLGADPKNTNYAGGNTSAKGTETDPVTGEP
                     VELVWVKGSGGDLGTLTPAGLAALRLDRMRSLVNVYPGIDREDEMVAAFDYCLHGKGG
                     AAPSIDTAMHGLVDAPHVDHLHPDSGIAIATASDGEELTPTIFGDKVVWVPWRRPGFQ
                     LGLDIAAIKTANPQAIGCILGGHGITAWGDTTDECEANSLWIIDTAAAYIAEHGKAEP
                     FGAVRPGFEALSSDERLAKAAALAPTLRAIASHDRAMVGHFTDAEVVLDFLASANAPA
                     LAELGTSCPDHFLRTKVKPMLLDLPADASVEDSIARLGELHQEYRADYAGYYERNATP
                     DSPAIRGADPLIILIPGVGMFSYGANKQTARVAGEFYVNAINVMRGAEALSTYAPIDE
                     SEKFRIEYWALEEAKLQRMPKPKPLATRIALVTGAASGIGKAIAERLAAEGACVVVGD
                     LDLAKAQAAAEGIGNADVAIGLVCDVTKPDQVQAAVDAAVLAFGGLDIVVNNAGLSLS
                     KPLLETTEADWDLQHNVMAKGSFLVSKAAAKVLIEQKLGGDIIYISSKNSIFAGPNNI
                     AYSATKADQAHQVRLLAAELGGHGIKVNGINPDGVVRGSGIFAGGWGASRAAVYGVAE
                     GDLGKYYAQRTLLKREVLPENIANAAFVLCTSDLSHTTGLHIPVDAGVAAAFLR"
     gene            complement(15109..16122)
                     /locus_tag="PLF56_00135"
     CDS             complement(15109..16122)
                     /locus_tag="PLF56_00135"
                     /inference="COORDINATES: ab initio
                     prediction:GeneMarkS-2+"
                     /note="Derived by automated computational analysis using
                     gene prediction method: GeneMarkS-2+.
                     GO_function: GO:0003677 - DNA binding [Evidence IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="MarR family transcriptional regulator"
                     /protein_id="HOQ52001.1"
                     /translation="MPPTELQRLTAAFVVSVRRAAIGPRARRHVKRLTGLEGSGLELL
                     VLMSINGHDLVPLGQVAGDLRVGAPMLSRACRQLEGRGELIRLADPRDRRRTLIGLSE
                     HAAAALARWNHEWPLRYAEPVLGWNEADRDALARWFAAVNLDLWDRSVEPHDRYAWRP
                     LDRAEFATPGMIRFANVVRDLVTQVGRFDEVHRIVRGLGVDLPEPAFFALMDLAEQPA
                     GVSGGEGVRVSELARAQAVDPALARRTLAPLAADGLVEQRQGRDAVARVTLSPRGADL
                     VQLVLAELASGLPQEPLIAPPAGLATLLARYLDVVLRDDPHDADGTIPPLLLDGRQRT
                     ALP"
     gene            16457..>17062
                     /locus_tag="PLF56_00140"
     CDS             16457..>17062
                     /locus_tag="PLF56_00140"
                     /inference="COORDINATES: protein motif:HMM:NF028442.2"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="glycosyl hydrolase"
                     /protein_id="HOQ52002.1"
                     /translation="MPIQSRSRARSLTVRALAVALLAGAATTGAAPPAGAETPRPDPR
                     HSPPAASGSKASFANPALADRPLYRFWNSGGMMTAESIAAQVAQMKAIGAGGFEANQL
                     AGIPGLSSMPGYDAATHAFGTPEWTRAWTQLFQAGTAAGLEVSQIYTPGWSAGIQGIS
                     PDAKGTAKEITFGSATLDGGATFNGALPTAPLPTGVTKRVLQ"
BASE COUNT         2598 a         5970 c         5845 g         2649 t
ORIGIN      
        1 gtcgtcgttc tcgaccaagc ccggcacctg ggtcaaagac atcgaagtca aggagggcac
       61 cgcaatggaa cagccactga tcacgggggt ggcccacgat cgcagcgagt cgaagatcac
      121 ggtggtcggg gtgcccgacc gggtgggtgc cgccgccgag gtcttcaccg cggtggcaga
      181 ggccggggtg aacatcgaca tgatcgtgca gaaccagtcg atcgccaacg acaacaccgc
      241 gatcacgttc acgttgcccg gtggcgacaa gctcaccgcg ctcgacgccc tgcacggcgc
      301 ccgcgaacgc atcggcttcg tggacacgct ctacgacgac gcgatcggca aggtgtcggt
      361 gatcggggtc ggcatgcggt cgcatccagg cgtgtcggcc accttcttcc gcgcattggc
      421 cgaggccggc atcaacattc agatgatcag cacctccgag attcgaatct cggtggtggt
      481 tgcgctgacc gacgtggacg ccgccgtgcg cgcagcccac accgccttcg ggctcgattc
      541 ggtcaccggc gaggccgtgg tctacgcggg caccgggcgc tagcgcgtcc acatcgctgg
      601 tcggggtggt cagcgcctgc cgccagtggc gtgcccgcag ccgacgtgca gaacgaagcc
      661 ggacgctgca cgcggatcgc tgattgtcgg gacccccaca ccagcgtccg tcgacccagc
      721 cacccccctg ccggtgatcg tccgtgacgc tgcccgggcc aggtgaaatg gcttgcgttg
      781 caacgcgggc aatgtcaacg cgggcaatgt caatgcgggc aatgtcaccc ggcgcgggcg
      841 acgtcacacc ggggccgtca tcgcagtcga ccggccgcat gatctgacgt tgcgcggtgc
      901 agcgctcatc gcgcgtggac ggacccgcgc aaccagccct tgaccgcgca acgacgcgcg
      961 gtcctgggct catgccgcgt gaacggagcc aggaaccgtc cccgactcca ttcactaccg
     1021 gtgtctcgac acgctcgagc gcgggcagtg agccacccga ggccccggcc caacacaggc
     1081 cagggattct gaattcctcc gcaggggtcg cggccgttgt cggcgtgtcg cgggtcgagg
     1141 agtggactgg ggtcgagatt tcagaacgag cgatccctgg tggccgagac cgcgaccgcg
     1201 accccgctca ggtcagccca tgccgctccc tggccgtact ggtcaccgtc tgcagaaagt
     1261 cgcccgcgcc gctcggcgcg ccggcaccca gccagccgga gggcacgtcg gccacggcca
     1321 actccacgcc ggtgtcgacc agcaccatcg gcagggtgtg catcaccgtc gaggggtagc
     1381 tgaccatcag ccgtccgacc gggccacgtc gcaccgcgat ctcgagcggc acgtcggggt
     1441 gcaccacctt gagcttcgtc gccgtggcga tccgtgccag cttggcctcg gattcacggc
     1501 ggtgggcgaa gtagcgcccg gctccgaatc gctcggtgac cgcctgcacg ccgaccaggt
     1561 aacgctcttc gttcaccacc ccggtctcga ccagtgaggt gccgatcacg tcgacggcgt
     1621 ccttgatctt gggcgggccg aagcggccgc gggtccaggc gaaggtgttc gcggtctgcg
     1681 tgatcccctc gggcgcctgc accggcatgg cggtgaacac ctcaatgctc cgttcgtcgc
     1741 gggtggacgg gctgaagaac tgacgggcgt gatccgacag caggtcgacc accacgtcac
     1801 gtccggtctc agccaggtgc cagcgggcca ggcgttcgcc ccgggtcagc tgcgagatga
     1861 acgaaatggt cgcggtgccg tcgtccacca ccacgacctc ggccacatcg gccagcggca
     1921 gcaccgcctg cacgaaccgc gagaacgggt cacccaccac cagccggctc gccttggcca
     1981 gccgcgggaa caactcccac gccaccgcca ccaaggaggc cgccgacctg cgcggttcgt
     2041 accaggtggc ggccagcccc gcgtcgcggg ccaggtcggc catcgcctcg agttgcagcc
     2101 gggtggtggc ttcgcgcggc ggcagcaggg ccaacctggt ctgcttctcg gccccgtgat
     2161 ggtgcgccca ctcgatcacg ttgagcaact gggccgggct ctcgaccagt gccagggtgt
     2221 cccgcttgtg cttgcggtgc ttggtggcca tggccggctc aggccacctg cgtcgcggcg
     2281 tccgccacca cgccgggcac ccggcgcagc ttgcgcatcg cggcgtactc gccctcgtag
     2341 acccgtttga cgccgtcacc gagcgcctct tcgatcaccc ggacgtcgcg gaccagccgg
     2401 ctgaggccgc ccggttcgag cgaagcggcc tggtcggagc cccacatggt gcgatcgagg
     2461 gtgatgtgcc gctcgatgaa gcaggcgccg agcgccgccg ccgccacggt ggtctggaga
     2521 ccgcgttcgt gacccgagta gccgatcggc acgttggggt actcggtgcc gagggtgttg
     2581 atcatgcgca ggttcagttc gctgttctcg gccgggtagg tggacgtggc gttcaccagc
     2641 accacgttgc tgctgcccaa cacctcgacg gcgtgacgga tctgcgacgg cgtcgacata
     2701 ccggtggaga gaatcaccgt cttgccgtac gcgcggcatg cgcgcagcag ttcgtcatcg
     2761 gtcaggcagg ccgaggcgat cttgtgcgcc ggcacgtcga agccgttcag aaactcgacg
     2821 ctgggcacgt cccagggcga cgcgaaccac tgaatgccct tgtcgcggca gtactcgtcg
     2881 atcacccggt actcgtccac accgaactcg atgcggtgcc ggtagtcgat gtaggtcatc
     2941 cggccccagg gcgtgtcgcg ctccacgtcc cactggtcac gcggcgtgca gatcttcggg
     3001 gtgcgctttt gaaacttgac cgcgtcagcg ccggcctcca gcgcgacgtc gatcagggcc
     3061 aggcaggtgg cgacgtcgcc attgtggttg aggccgatct cggcggtcac gtagaccggg
     3121 tgattggggc cgacggggcg gtcgccgatc aggcgttcgc gggtggtgtg gatcatgagg
     3181 tgagttcctt tccgaggatc caggaggcga tttcacggac ggctccgtga ccgccgggca
     3241 cagaggtgac cagccgcgcg gcatcgcgca cgaccggatg agcggaggcg accgcgaccg
     3301 gccagccgac gacctcgaag cagccgaggt cgttcacgtc gttgcccgcg tacagcaccc
     3361 gggcgggatc gatgcgttcg gtgctgcacc agtcgcgcag cacggtgccc ttgtcgtcca
     3421 caccgtggat caccggcacg ctgagcttgc gggcgcgggc gcgcaccacc gggttcacct
     3481 cgctggagag aatcagcacc ggtatgccgg cccggcgcag cgccgcgatg ccgaggccgt
     3541 cgccgcggtg cacgcggacc tgctcgctgc cgtccgagcc gacctgggcc gagtcgtcgg
     3601 tctgggtacc gtcgaagtcg aggacgacgg cgtcgatctc gcttctgccc ggcacggacg
     3661 caggcacctc gaccaggtgc gccagggtgc gcgcccgctg caggtcggag gtttcgtcga
     3721 tctcgagcac ccgcgcgggg tcggtctcga tcagcgcggt ggtgccgaag aaccgatgcc
     3781 ggtgctgccg aaatccgtcc gccgacatgg cgtagacggc gccggtctcg agaaactccg
     3841 gctcgcggtc ctggcggcgg ggacgggtcg ccttgtcatg attgacgccg accgcgttgc
     3901 cggcggcgtc gcgtcgccag agaaacccgt gactctgcgc ggcggtgaaa gcggtgtcgg
     3961 cgccgccacg cacggcctcg acgcaggcgg cgacctcgtc cggggtgaga aaggggctgg
     4021 tgcactgcag catcaccagc acgtccacac tgtcaccggt cagtcgctcg aaggcgtcca
     4081 gggcgtgcag cagcgccgac tcgctggacg cctggtcgcc ggccagttcg tccggccgcc
     4141 tgatcaccag ggcaccggcg gactcggcga tcgcggcgat ccggtcgtcg tcggttgaga
     4201 cagccaccgc atcgacgcgt ccggacgcct gcgccgcacg aatcgctcgg acgaccagtg
     4261 gcacaccggc caccggcgcc aggttcttgc ccggaacacc cttggagccg ccgcgggccg
     4321 ggatcaccgc cagcgcagtc atatgcccgc caatcgccgc agggtggggg cgacggtgtc
     4381 gacgccctgt cgataggccg tgcgcgcagc ccgtgagacc agggagcgca ccgcgtgggg
     4441 caggtcggcg agcagggcgt cgtcggtttc gagcgcgtgc ccgtgcccgt ccaggtcgta
     4501 gcgacccagc agttgcggca ccatgccagg agcgttctgc atcgaataga acggacgaag
     4561 cggtggccgg acctgctcgg gcagccgcgc ggccagcgcg gcccgggcct gttcgacaac
     4621 gtcactggcg cccagccctc gcgagcgggc ccaaccggaa tcgacggtgg gcagcaatcc
     4681 atcgtccagg tcggcgaagc cggccaggca gccggagccg atgaagtagt ggttgcccag
     4741 cacctctttg atgccgaagt cggtcaggat cgcggtcggg atgccgcggt gcatcgcctc
     4801 gacagccgcc gtggacgaca ccgtgaccag caggtcggtg cgttgcagca cctcgcccat
     4861 gttgccctgc acgatgcgaa agttggccgg cggctgcgtg ctcagcttgg ccgcaagccg
     4921 ctggtagggg tggtagtcgg cgtgcgtgct gcgctcgccc aacgcgccac gcaccttgag
     4981 cagcacctcg cggtcggggt gcagccgggc gtgccgaaac agccggcgca gcacgagttc
     5041 gcgctcagca cgggtgagcg gcacacccgg ctgcgcggcg aacgtcacgg tgaacggccc
     5101 gtcgtgcgga cgcgccgagt caggttcacc cagaaacggc agcaccgttt cgagcaccac
     5161 ggccgggtcg gcgccggtgc cgtcgagcag ccggcgaaac tcctctgcgt cggtggcgct
     5221 gttggccagg atcacgtccg agccggcccg cagcagcagt ccctcggcga gcttctcgta
     5281 caccacgccc acgtagccgc tgatcaccaa cggacggtga cccgcccgcg gccaccggcg
     5341 ggccaggccg tgcagcaccg cctgtacggc accaccaggc agggcgagca cgagtgcgtc
     5401 cacctcgtcg aggtcgtcca gttcgaggaa gtcgggaaca ccgccgacga cgtccggctg
     5461 aaccgtcacc cccgtgtcgg ccaactgccg tgccgaggga acgctggcgc tttcgagcag
     5521 cagcccggtg tgcgtcgcag cgggaaacag ctgcttggcc gtgaggaatc cccacttcca
     5581 gcggctgtcg gtgtcggcca gcaccagtac ccggatgggc ggcgtctgga cggacgggtc
     5641 tactgcggtc atgtttcgac gctaagtagc ccgaccgaca cgatggccaa cgagaagcag
     5701 atgtctggtg aactcctgcc aacgtccggg tcacggcgtt cggttgcggc cgcggagttc
     5761 accaacacgt catggtggcg ttgcctggcg gacaccggaa ccggacagca tcgatcgagt
     5821 gcgggtcgcg ggggcgtccg tcgaacggag gccgagccga tccgaaggag tcgaagctgt
     5881 gaagttgtcg gtgatcattc cctgctacaa cgtggccgaa cagttgccaa cgttggtgcg
     5941 cagcctgcag ggcaacctgc gccgcgactt cgagttcgtt ctggtcgagg actgctccgc
     6001 cgatgacacc ccgagcgtgc tggccgagtt gatccccact gtgccgggag cgcgcctggt
     6061 acagcatgac cgcaaccgtg gtatcagcgc ggcacgcaac agcggcctcg cggtcgctca
     6121 gggcacctat ctggtgtggc tcgactccga cgactggatc gggccgggct atctcgacca
     6181 gttggtgcgg gtgatcgaac ggctcggctg tgacttcgtg cgtaccgatc atgtgcgagt
     6241 gctggggcgg cgacgcacca tcgtgcggtc gccggaatcg cgccgcaacc tcgtgctgcc
     6301 gccgcgcacc ggcatcgcgc ccccgtttcg ctccacgtcg gtggactacc cgaacgtgtg
     6361 ggccggcatt cagcatcgcc ggctcgcgac acagggcctg ctcaccttcg acgaagacct
     6421 gcgcacctgc gaagaccggc actggatctg gcgcatgcat cgcgacgcgc agtcgttcgc
     6481 cacggtcggg ctgctcggcc tgttctaccg gcaggggctc acacaatcgc tcagccacat
     6541 ctcgagcccg gaccaactgc acttcctgga cgccatgcag aaggtcgtcg acgaaacgct
     6601 ggccgacccc accggcgaac cgttgctact caaggcggtc gacgcctact gcgcgctgat
     6661 caactttcac ctcaccaaac gcggccggct caccccggcc ctccagcgtg agctgatccg
     6721 gcgcagcatc ctcgctctgg acggcctgcc acaggacctg ctcgaccagg cgctcaaaga
     6781 gggcgcaccg agacgccgca cgctgctcac ccggatgcgc cgcaaccccc agtacctgaa
     6841 ggaggtcgcg tgatccagct gtgtgtcgcc agcaccctgt atggcaccgc caccctcgcc
     6901 gcggccgtcg atgccggcct gctgggaacg cccgagcgac gggtgctcgt gatcagcaac
     6961 aacgccgagg cgccggaggc ccaacccggc ccgcaggccc agcccgggtt cgccgagttg
     7021 gccggacgct tcgacgccgt ggtgtcgtgg aacgacgtgg tgtacccgtt tcaccccacc
     7081 cagtggcgcc cccgcgccag cgacgcgacg ctgtgggagc ggctggtgcg gcaggcgtgg
     7141 cagctggacg agggccccct cgaactggtc atggagtcga ttcaggcgcc gcccgccctc
     7201 gccctggccg gcctgtttct ggacgccccg atccggctct actccgacgg gctgatgacc
     7261 tacggcccca cccgccgggt gctcggcgat cagatcggca gccggctgat gcaggtgctg
     7321 tacctggagc tgattccggg cctggagcct ctggttgcgc acgagtacgg cccggccacc
     7381 atggcgattc cgcacgcggc gatggccgcg gtcctcgccg aggtgacggc cgcgaccccc
     7441 gccacgcccg gactgcccag cggctgtgcg ctgatcgtgg ggcagtacct gtcggccgcc
     7501 gaactgatca gcgaagacca ggagcgcgcc ctctacgtcg acatggtgcg tcgggcggtg
     7561 gccctgggtc atcgcgacat cgtgttcaaa ccgcatccca cggctccacg gcactggcag
     7621 cagcacctca gtgccgcggc gagcgccgag ggcgcccggc tcagcttcgt gaccacgccc
     7681 gctctggccg aggtgctgta cgaacggctt cgtccggcgg tggtgatcgg ttgcttctcg
     7741 accgcattgc tgacggcctc cgcgttctac gggttgcccg tgggacaact cggcacggcc
     7801 ctgttgttcg agcggctcgc gcccttcgcc aacagcaacc gggtgccggt cgtgctggtc
     7861 agcgcgctgg tgcccgatct tgcggccggc gcgacgcccc gtccggtcag taccgacgcc
     7921 gccggcggca tcgacgctct gcggcggctg gtcgcgaccc tcgcctactg catgcagccg
     7981 gcgatctgcg acgatctgcg aggcatcgcg gtcgacgaac tgcgcacgaa accgtccgtc
     8041 taccgccggc tgatctcgcg cggacggctg gcccgcctgg gtctgcccgg cgcccctgtc
     8101 gacctgagct ttctactcgg caaccggcat gtgcggcgtg gggcccaggg cctgctggca
     8161 gcgcccaaac gggtgcgccg caagctggcc cgcgcgctga actgagcacc gagcttttcg
     8221 ccggtcggct tgccgggccc tttgcttgcg tcatcccggc ctattccgtc atcccaagac
     8281 tcacagccgg gatctctccg cgtcgagacc atgccgcggg tcgaacgtcc aggaacccag
     8341 ttcacccgca tcacccgtag ccagccacag cgcaggccga gaacgaacag cgttgagaac
     8401 cgtccgcaac ccacgtcacg gacgagcgtc ggcccggccc ctgacgagac cggccgctgc
     8461 ctactcgttg accagcagca cgtgcaaggt tctcggcccg tgcacgccct cgacccggtt
     8521 gagttcgatg tcgctggtgg cggacgggcc gctgatccag gtcaacgcgc gccgttcatc
     8581 aatggacgct tgcagccgcg cgacggcctc gggtacgtcc gagaccacct ggtcggcccg
     8641 gatcacgcag acgtgcaggt cgggcaccag tgacagggcg cgccggccct ggtcggcatc
     8701 gtggtcgagc acgatggtgc ccgtctcggc gatacccacc cgggaggcgg tgaccacccc
     8761 gccggtcgcg tcgagctcgg cgtggctgag caccggatcg tccgtcacca ccgcgatgcc
     8821 cgccgccgcg accgcctcat gccattcgcg cggggcgccg gccggcacca ccgccgaggt
     8881 ggccccgtga tcggccaggg cgttggcgat gcgtgccggc agctcggccg gcaccaccca
     8941 actgacgatc gccttgtagt cctcgacgcg ctcaacgaac aggccgatca tgtcgtcggc
     9001 ggccgtgtca ggacgctgcc cgtactgcca gttcacctcg acgtcatcgg cggccgacac
     9061 cttcggcacg tcgaccagtg cggagcggat acggcccagg atggcctctt tgctgctcat
     9121 catgcgtctt tctgctcggc gtcgcccttg cgcttggcct tctcgttctt gacccaccag
     9181 tcacggaagg tctcggtcgg cggcttcttc aggtcgcgag caccggtcca ggcgccggtg
     9241 ccgggaatgt ggccaacagt cgcgccgggc cagaccgcac cgaacaactc cgcgcccttc
     9301 tcgagcgtgc cgaaccggct gcccttgccc atcacccagc cgccggcctt catgccgatg
     9361 ctccaggcgc tgggcccgtg atgctcttcg accaccttgt tgcgcaggtg caccagcacc
     9421 tcgggaatgt tgatccgcac cgggcagacc tcgaagcagg cgccgcacag cgaggacgcg
     9481 tacggcaacg actgatcgac ctcggacgcg gtgccccgca gctggggggt caggatggcc
     9541 ccgatcgggc ccgggtacac cgagccgtag gcgtgcccac cgacccgctc gtagaccggg
     9601 cagatgttca ggcaggccga gcagcggatg caacgcagcg cctgccgtcc gacctcatcg
     9661 gccagcacct tggtgcggcc gttgtcgacc agcaccacgt gcacctcctg cgggccgtcg
     9721 ccggcctgca cgcccgacca catcgaggtg tagggattca ttcgctcggc cgtggacgag
     9781 cgcggcagca gttgcaggaa cacctcgaga tcggcgaatg tcggcagcgt cttctcgatg
     9841 ccgacgatcg agatcagtgt ctcgggcagg gtcaggcaca tgcgtccgtt gccctcggac
     9901 tcgaccacca ccagcgtgcc ggtctcggcg atggcgaagt tggcgccgct gacggccacc
     9961 ttggcgcgca gaaacttgtt gcgcaggtgg acgcgtgccg caccggccag catggccggg
    10021 tcgtcggtca gcccctcggg cggcggggtg ccgtagcggc ccatctcttc gagaaagatc
    10081 tcgcgcacct ctgatcggtt gcgatggatc gccggcacca gaatgtggct cggacggtcg
    10141 tggcccagtt gcacgatcag ctcggccagg tcggtctccc aggctgcgat gccggcctct
    10201 tcgagcgcct cgttcaggtc gatctcttgc gtggccatcg acttgacctt gaccacctcg
    10261 tcggcgccct tggatcgggt gatgtcgatc acgatctggt tggcctcggc cgcgtcgttg
    10321 gcccagtgca cgatggcacc gttggcggtc agggacgcct cgagctgcag caggtagtcg
    10381 tccagatggc gaagtgtgcg gttcttgatc gcctcgccgg ccaaccgcag gtcctcccaa
    10441 tgcggcaact cgttgaccac cttgacgcgc ttgttgcgaa tcgtggtggt ggcgtgccgc
    10501 aggttgctgc gaagttgggc gttggccagc tctttcttcg cgttcttggg aaacttcggc
    10561 atgccgatca gggtgaccgg cggcggcgcg ggggtgcggc gctcgtgagt caccgcggtg
    10621 tcctgcacgg tcttggtgcg atcggggctc atcgcacgcc tcctgcctgg gtggtcgccg
    10681 agccggccgg cacgaggtcg gcctcggtgc tggccaggat ctcggccaga tggatcgtct
    10741 tgacgccggc ctcgttgcgg ctgagcagcc cgccgacgtg cagtaggcag gagttgtcgc
    10801 cggcaaccag gtattcggcc ccggtgtcga gcacgttgcg catcttgtcg gtacccatcg
    10861 cgacggaggt gtcggggttc ttcaccgaga acgtgccgcc gaagccgcaa cactgatccg
    10921 agtcgttcag ctcgatcagc gtgagcccct tgaccgcccg cagcaacttg taggggcgat
    10981 cgccgacctt cgtcacccgc agtgaatgac acgtcgagtg ataggtgacg gtgtgcggaa
    11041 agtaggcacc gacatcggtg acctcgagca cgtccaccag aaactccgac aggtcaaggg
    11101 tcttggcgac gatggcgtcc acctcgcgct gcagcccggg atcgcccagc cgcttcgcca
    11161 gcatcgggtg ctggtcgcgc accgaaccga tgcacgaacc ggacggcccg accacccact
    11221 cgtacggacc gaaggtgtcg acgaagttgc gcaccgacgg catcgcctgc ttgaagtagc
    11281 cggtgttggt gaacatctgg ccgcagcagg tctgctgcag cggaaagtcg acggtgcagc
    11341 ccagccgctc cagcaaccgc agcacggcct tgggggtgtc ggggaacatc atgtcgttga
    11401 tgcacgtggc gaacagggcc acggtgccgg tcgggacgct catcgtcctc ctctgcggtc
    11461 gatactgcgc gggcgggtcg gcagaattgc tggagaagtg ccgatcgatg cgcgatcttt
    11521 cttgtggttc gtccgaacca ctgcgcacgc gggtctcgac gagctcgacc agcgattgat
    11581 caccgcttcc ttaggtcagt ctgctgcacg atgccggggt gcgtagcgaa tcgggtcaaa
    11641 cgaccgagca atgatctgcc ggatctcgtc cagtgagccg acccggccgt gcgcccgggc
    11701 ctgaaccagc acgttgccca gcgccgtcgc ctcgatcggg ccggccacca ccgcaaggcc
    11761 cgagcggtcg gccgtcagct ggcacaacag ctggttctgg cagccgccgc cgaccacatg
    11821 gatctgctcg acgcgcaccc cggacagttc cgcggcggtc cgcacggcgg tcgcgaagtg
    11881 ctcggccagg ctctcgatga tcagccggac gatcgccacg tccgaacccg gctggggcag
    11941 gccgcgctcg tccagccact cgccgatgcg caccgccatg tcgcccggcg gcaggaaccg
    12001 gtcgtcgttg aggtcgacca ggtgcggcgg tgcgggcact tccgcagccg cggccagcag
    12061 ggtgccgagg tcggccgaac cgcccctgga gcgccagacc cgcagcgtct ccgacagcac
    12121 ccacaggccc atcacgttgt gcagaaaccg ggtggtgccg tccacgccgc cctcgttggt
    12181 gaagttggcc gctcgtgcgg catcggtgag caccggggcg ggcagttcca cgccgaccag
    12241 gccccaggtg ccgcacgaga tgtaggcggc gcgggtgggg tccatcggca cgccgaccac
    12301 cgctgcggcg gtgtcgtggc tgggcaccga gacgacgtcc aggctctggc cgatctcttc
    12361 gaccagcgtt ggacgcagcg acccgacggt gaccccgggc tgaacgagtg cgggcaacag
    12421 gccctccggg tagcccaacc gggcgatcaa ctcgcggtcc cactgccggg tgcgggcgtt
    12481 caccaggccg gtggtggacg cgttggtgta ctcagccacc tgacgtccgg tcaaccagta
    12541 ggcgagcagg tcgggcacca gcagcaggcg ctgcgggtcg cgcgggccgt ccccgctcag
    12601 gtcgggttcg gcggccagtt ggtagagcgt gttgaagggc agaaactgca ggccgttgcg
    12661 tcggtagagc gcgtcgaagg caatcgtggc gtgcaccttc tcgacgccgc gggccgtccg
    12721 ggcgtcgcgg taggcgtagg gggtgccgac cagaccgcca ccgccgttga gcagcccgta
    12781 gtcgacggcc cacgagtcga tgccgaccga ggccagcgga tgccgcgcgg ccgcgtcgtg
    12841 caggccggtg agcacctcgc tgtacaggcc gagcaggttc cagtgcagcc cgtcgggcaa
    12901 ctcgacgacg ccattgccga agcgggccac ctgctcaagc tcgacccgtc cgtgcgcgag
    12961 gtggccgacc atcacccgtc cgctgctcgc gcccaggtcg accgccccga cgctcagttg
    13021 cgtggtggcc accgggtcac cgcaggaacg cggcggcaac gccggcgtcg acgggaatgt
    13081 gcaggccggt ggtgtgcgac aggtccgagg tgcacagcac gaacgcggcg ttggcgatgt
    13141 tctcgggcag cacctcgcgc ttgagcaggg tgcgctgggc gtagtacttg cccagatcgc
    13201 cctcggcgac gccgtagacc gcggcgcgcg aagcgcccca gccgccggcg aagatgcccg
    13261 agccgcgcac cacaccgtcg ggattgatgc cgttgacctt gatgccgtgg ccgcccagtt
    13321 cggccgccag cagccgcacc tggtgggcct ggtcggcctt ggtggccgag taggcgatgt
    13381 tgttcgggcc ggcgaagatc gagttcttgc tgctgatgta gatgatgtcg ccacccagct
    13441 tctgctcgat cagcaccttg gccgcggcct tgctgaccag gaagctgccc ttggccatca
    13501 cgttgtgctg caggtcccag tcggcctcgg tggtctcgag cagcggcttg ctcagcgaca
    13561 gcccggcgtt gttgacgacg atgtcgaggc cgccgaaggc gagcacggcg gcgtccaccg
    13621 cggcctgcac ctggtcgggc ttggtcacgt cgcagaccag gccgatcgcg acgtcggcgt
    13681 tgccgatgcc ctcggccgcc gcctgcgcct tcgccaggtc gaggtcaccg accacgacgc
    13741 aggcgccctc ggcggcgagc cgctcggcga tcgccttgcc gatgccggac gcggcgccgg
    13801 tgaccagcgc gatgcgcgtg gccagcggct tcggcttcgg catgcgctgc agcttggcct
    13861 cctcaagcgc ccagtactcg atgcggaact tctcgctctc gtcgatcggg gcatacgtcg
    13921 acagggcttc ggcgccgcgc atcacgttga tcgcgttgac gtagaactcg cccgcgaccc
    13981 gggcggtctg cttgttggcg ccgtagctga acatgccgac gccggggatc aggatgatca
    14041 gggggtcggc gccgcggatc gcggggctgt cgggggtggc attgcgctcg tagtatccgg
    14101 cgtagtcggc gcggtattcc tggtgcagtt cgcccagcct ggcgatggag tcctcgacgg
    14161 aggcgtccgc gggcaggtcg agcagcatcg gcttgacctt ggtgcgcaga aagtggtcgg
    14221 ggcaggacgt gcccagctcg gccagcgccg gggcgttcgc tgaggccagg aagtcgagca
    14281 ccacctcggc atcggtgaag tgaccgacca tggcccgatc gtggctggcg atggcgcgca
    14341 gggtgggggc cagcgcggcg gccttggcca gccgctcgtc ggagctcagc gcctcgaagc
    14401 ccggccggac ggcgccgaac ggctcggcct tgccgtgctc ggcgatgtag gcggcggcag
    14461 tgtcgatgat ccacagggag ttggcctcgc actcatcggt ggtgtccccc cacgcggtga
    14521 tgccgtgccc gcccaggatg cagccgatcg cctgcgggtt ggcggtcttg atcgcggcga
    14581 tgtcgagccc gagctggaag ccgggccggc gccacggcac ccaaaccacc ttgtcgccga
    14641 agatcgtcgg ggtgagctcc tcgccgtcgg aggcggtggc gatcgcgatg ccggagtcgg
    14701 ggtgcaggtg gtcgacgtgc ggggcgtcca ccaggccgtg catggcggtg tcgatggacg
    14761 gggccgcgcc acccttgccg tgcaggcagt agtcgaacgc ggcgaccatc tcgtcctcgc
    14821 ggtcgatgcc ggggtagacg ttcaccagcg agcgcatccg gtccagccgc agggcggcca
    14881 ggccggcggg ggtcagcgtg cccaggtcgc caccggagcc cttcacccac accagttcga
    14941 ccggctcgcc ggtgaccggg tcggtctcgg tgcccttggc ggaggtgttg ccgccggcgt
    15001 agttggtgtt cttgggatcg gcgcccaacc ggttgctccg gctgatcagc tggtcgactg
    15061 ccacgttggc catcaggtca ctcctgtgtg atattgaatc gtttcaactc agggtaacgc
    15121 ggttctttgc cgtccgtcaa ggagcagcgg cggaatcgtg ccgtcggcat cgtgcgggtc
    15181 atcgcgcagc accacgtcca ggtaacgcgc gagcaacgtc gcaagaccag caggtggggc
    15241 gatcagaggc tcctgcggaa gcccgctcgc gagctcggcg agaacgagct gcaccaggtc
    15301 cgcacctcga ggactgaggg tgacccgagc caccgcgtcc ctgccctgcc gctgctcgac
    15361 cagcccgtcc gccgccagtg gggccaaggt gcgccgggcc agtgccgggt ccaccgcctg
    15421 cgcccgcgcc agttcgctta cccggacgcc ctcgccgccg gacaccccgg caggctgctc
    15481 ggccaggtcc atcagggcga agaaggccgg ctcgggcagg tccaccccca ggccccggac
    15541 gatccgatgg acctcgtcga agcgtcccac ctgcgtgacc aggtcgcgca ccacgttggc
    15601 gaaccggatc atgccagggg tggcgaactc cgcccggtcc agcggacgcc aggcgtaccg
    15661 atcgtgcggc tctaccgaac ggtcccacag gtcgaggttg acggcggcga accagcgtgc
    15721 gagggcgtcg cgatcggcct cgttccagcc gagcaccggc tcggcgtacc gcaacggcca
    15781 ctcgtggttc caccgggcca acgctgcggc ggcatgctcg gacaggccga tcagcgttcg
    15841 gcgccggtcg cgcggatcgg cgagccggat cagctcaccg cgcccctcga gctgacggca
    15901 cgcccgtgag agcatgggag cgccgacccg taggtcgcct gccacctgcc ccagcggcac
    15961 caggtcgtga ccgttgatcg acatcaggac caggagttcc agtcccgacc cctcaagacc
    16021 ggtgaggcgc ttgacgtggc gtctggcgcg cggcccgatc gcggcccgtc ggacgctgac
    16081 gacgaaagcg gccgtcaatc gctgcagttc ggtcggcggc actcgaccat ggtcgcgcac
    16141 tctcgccccg cgccggtccc gccgcgccac tgattcggcc agcgcacgct cggactttgg
    16201 gtaaccaatc gtcgcgcaca ccttgaccga cccccgttca cccgcctact ctcgagtcgg
    16261 cccggttgta acgtttcact ccttcgcgct ccatctcacg cgcgtaccga cttctccctg
    16321 cgtcgtgacc cacaaggctc acgccccgac ccgaaccacg ccacacaacc cgggccactg
    16381 gaagcccttc gcagcgcccg tcgtcacagt gatatcgatc agcggcgcga cgaccaccgt
    16441 ctcaacgagg agaaccatgc caatacagtc ccgatcgagg gcacggtcgc tgaccgtccg
    16501 agcccttgcc gtcgcgctcc tggcgggtgc ggcgaccacc ggcgcagccc cgccggccgg
    16561 cgccgagacg ccacgaccgg atccccgcca cagcccgccc gcggcgtccg gttcgaaggc
    16621 gtccttcgcc aatccggcgc tcgccgaccg tccgctgtac cggttctgga actccggcgg
    16681 catgatgacc gccgagtcca tcgccgccca ggtcgcccag atgaaggcca tcggggccgg
    16741 cgggttcgag gccaaccaac tggccggtat tcccggcctc agcagcatgc cgggctacga
    16801 cgcggccacg cacgcgttcg gtacgccgga atggacgcgc gcctggacgc aactgttcca
    16861 ggccggcacc gccgcgggcc tcgaggtcag ccagatctac acacccggct ggtcggccgg
    16921 cattcagggc atctcgccgg acgccaaggg cacggcgaag gagatcacct tcggctcggc
    16981 gaccctggac ggcggcgcga ccttcaacgg cgccctgccg accgcaccgc tgcccaccgg
    17041 cgtgacgaag cgggtgctgc ag
//
LOCUS       DAOSUG010000003        16234 bp    DNA     linear   ENV 10-JUN-2024
DEFINITION  MAG TPA_asm: Micropruina sp. isolate AMR_MDS_2195
            NODE_2207_length_16234_cov_4.362692, whole genome shotgun sequence.
ACCESSION   DAOSUG010000003 DAOSUG010000000
VERSION     DAOSUG010000003.1
DBLINK      BioProject: PRJNA925477
            BioSample: SAMN32864262
            Sequence Read Archive: SRR4236673
KEYWORDS    WGS; ENV; Metagenome Assembled Genome; MAG; Third Party Data; TPA;
            TPA:assembly.
SOURCE      Micropruina sp. (wastewater metagenome)
  ORGANISM  Micropruina sp.
            Bacteria; Bacillati; Actinomycetota; Actinomycetes;
            Propionibacteriales; Nocardioidaceae; Micropruina.
REFERENCE   1  (bases 1 to 16234)
  AUTHORS   Abdulkadir,N., Saraiva,J.P., Zhang,J., Stolte,S., Gillor,O.,
            Harms,H. and Rocha,U.
  TITLE     Genome-centric analyses of 165 metagenomes show that mobile genetic
            elements are crucial for the transmission of antimicrobial
            resistance genes to pathogens in activated sludge and wastewater
  JOURNAL   Microbiol Spectr 12 (3), e0291823 (2024)
   PUBMED   38289113
REFERENCE   2  (bases 1 to 16234)
  AUTHORS   Abdulkadir,N.
  TITLE     Direct Submission
  JOURNAL   Submitted (24-MAR-2023) Environmental Microbiology, Helmholtz
            Center for Environmental Research -UFZ, Permoserstr 15, Leipzig
            04318, Germany
COMMENT     The annotation was added by the NCBI Prokaryotic Genome Annotation
            Pipeline (PGAP). Information about PGAP can be found here:
            https://www.ncbi.nlm.nih.gov/genome/annotation_prok/
            
            ##Genome-Assembly-Data-START##
            Assembly Method        :: SPAdes v. 3.11.1
            Genome Representation  :: Full
            Expected Final Version :: Yes
            Genome Coverage        :: Not Applicable
            Sequencing Technology  :: Illumina MiSeq
            ##Genome-Assembly-Data-END##
            
            ##Genome-Annotation-Data-START##
            Annotation Provider               :: NCBI
            Annotation Date                   :: 05/04/2023 04:57:10
            Annotation Pipeline               :: NCBI Prokaryotic Genome
                                                 Annotation Pipeline (PGAP)
            Annotation Method                 :: Best-placed reference protein
                                                 set; GeneMarkS-2+
            Annotation Software revision      :: 6.5
            Features Annotated                :: Gene; CDS; rRNA; tRNA; ncRNA
            Genes (total)                     :: 2,776
            CDSs (total)                      :: 2,750
            Genes (coding)                    :: 2,714
            CDSs (with protein)               :: 2,714
            Genes (RNA)                       :: 26
            tRNAs                             :: 24
            ncRNAs                            :: 2
            Pseudo Genes (total)              :: 36
            CDSs (without protein)            :: 36
            Pseudo Genes (ambiguous residues) :: 0 of 36
            Pseudo Genes (frameshifted)       :: 2 of 36
            Pseudo Genes (incomplete)         :: 32 of 36
            Pseudo Genes (internal stop)      :: 2 of 36
            ##Genome-Annotation-Data-END##
FEATURES             Location/Qualifiers
     source          1..16234
                     /organism="Micropruina sp."
                     /mol_type="genomic DNA"
                     /submitter_seqid="NODE_2207_length_16234_cov_4.362692"
                     /isolate="AMR_MDS_2195"
                     /isolation_source="Activated sludge of municipal
                     wastewater treatment plants"
                     /db_xref="taxon:2737536"
                     /environmental_sample
                     /geo_loc_name="Canada"
                     /lat_lon="49.25 N 123.1 W"
                     /collection_date="2015-06-16"
                     /metagenome_source="wastewater metagenome"
                     /note="metagenomic"
     gene            <1..724
                     /locus_tag="PLF56_00145"
     CDS             <1..724
                     /locus_tag="PLF56_00145"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_013865164.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=2
                     /transl_table=11
                     /product="heavy-metal-associated domain-containing
                     protein"
                     /protein_id="HOQ52003.1"
                     /translation="PTGTGESGELSFQILDADGTALTAYESSHEKDLHLIVVRTDGTQ
                     FRHVHPTLDEATGTWTTSWEWTEAGSYRIYADFVPDVADGPDKVTLTRTVDVAGAFTP
                     NPATATSTTSEVDGYTVTLDGDLTAGESSELTLSVTRDGQPVTTLQPYLGAFGHLVAL
                     RDGDLAYLHVHAEGDDPQDGDTAGPDIRFAAEAPTTGRYLLYLDFQVDGQVHTAEFVV
                     DAGHGTGDTDTDDTHSDTGDGH"
     gene            760..3033
                     /locus_tag="PLF56_00150"
     CDS             760..3033
                     /locus_tag="PLF56_00150"
                     /EC_number="7.2.2.-"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_020391618.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_component: GO:0016020 - membrane [Evidence IEA];
                     GO_function: GO:0005524 - ATP binding [Evidence IEA];
                     GO_function: GO:0019829 - ATPase-coupled monoatomic cation
                     transmembrane transporter activity [Evidence IEA];
                     GO_process: GO:0006812 - monoatomic cation transport
                     [Evidence IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="heavy metal translocating P-type ATPase"
                     /protein_id="HOQ52004.1"
                     /translation="MSTTAPAEQTADSAVELQIGGMTCASCAMRIEKKLNKLDGVAAT
                     VNYATEKAKVTIPAGYDPALLIAEVEKTGYSAALPAPKKAKNDTAEGGDADDADPELT
                     SLRNRLIVSIVLSVPVIAMAMVPALQFTYWQWASLALAAPVIVWAAFPFHKAAWANLK
                     HGTATMDTLISMGTTAAFLWSLYALFFGTAGTPGMTHPFELTLAPSEGAANIYLEVAA
                     GVTMFILAGRYFEKRSKRQAGAALRALLELGAKEVSVLRDGVETKIPVEDLQAGDEFI
                     VRPGEKIATDGVVVSGTSAVDASMLTGESVPVEVAEGDPVTGATVNAGGRLVVRATRV
                     GSDTQLAQMAKLVEDAQTGKAEVQRLADKISGVFVPIAILIAVVAMGAWLGAGFPVAA
                     AFTAAVAVLVIACPCALGLATPTALLVGTGRGAQMGILIKGPEVLESTRKVDTVVLDK
                     TGTVTTGKMTLVNVVTEPGTDADELLRLAGALEDASEHPIAQAIAKGATQQTGTLPTV
                     EGFTNLEGKGVQGVVDGHALIVGRESLLAEWSLRLSAEVAAAKAQAESEGKTVVAVGW
                     DGAARGILIVADTVKPTSKEAIAQLKALGLTPVLLTGDNEAVARQIAAEVGIDQVIAE
                     VLPKDKVDVVTRLQGEGKVVAMVGDGVNDAPALAQADLGLAMGTGTDVAIEASDITLV
                     RGDLRSAADAIRLSRKTLGTIKTNLFWAFAYNTAAIPVAALGLLNPMLAGAAMALSSV
                     FVVGNSLRLRGFKSIAR"
     gene            3083..3325
                     /locus_tag="PLF56_00155"
     CDS             3083..3325
                     /locus_tag="PLF56_00155"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_006213356.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="YHS domain-containing protein"
                     /protein_id="HOQ52005.1"
                     /translation="MSSCCSTSAPAPGGRENLLDAAGGADMTTCPVMEGTPVNKATAV
                     AAGLFRDYEGERYYFCCAGCGPAFDADPARYAAAMA"
     gene            3381..3677
                     /locus_tag="PLF56_00160"
     CDS             3381..3677
                     /locus_tag="PLF56_00160"
                     /inference="COORDINATES: protein motif:HMM:NF014624.2"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="metal-sensitive transcriptional regulator"
                     /protein_id="HOQ52006.1"
                     /translation="MTSTIAAAREPGYITDPRTYRNRLRRLEGQIRGLTGMIEQERPC
                     IDTLTQISATQRALQGVGLALLTDYLRHAHATGTLTDDTVLDAASTAITQLIRS"
     gene            3733..4884
                     /locus_tag="PLF56_00165"
     CDS             3733..4884
                     /locus_tag="PLF56_00165"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_006214163.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="NAD(P)/FAD-dependent oxidoreductase"
                     /protein_id="HOQ52007.1"
                     /translation="MPTHHVLILGAGAAGVAAATALARHEDIHVTLVGRTHETPYIRM
                     HITGVAFGPTLPETIHAPLPPVTVLADTATHLDPISRIVHLASGAQLRYDAVIIATGS
                     RPDTLTPGLPGRHTPAARHQVVTLHSIQDAQQIREHLTRTAGPSRVAIYGGGFTGAET
                     ASALHAAGHHVALISRSRIPGRASFGAQIAAHITGIHRGKITTFLGRTITAVRDRSPL
                     LGITLDDTTTLSADLLITALGNAPTAPGPFSSGVPVDDRLRSTAPHVYAAGSTAIHRD
                     DHLGIWRLDHWADAAAQGQHAAAAVLHDRTGAADPGPYRPRSSYTSLIHGTAITGIGY
                     TMTPATEGQGPGPGTLVTHTLNGALVGVSGVDAVATVHETAQHLHESAA"
     gene            4881..6128
                     /locus_tag="PLF56_00170"
     CDS             4881..6128
                     /locus_tag="PLF56_00170"
                     /inference="COORDINATES: protein motif:HMM:NF019310.2"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_function: GO:0022857 - transmembrane transporter
                     activity [Evidence IEA];
                     GO_process: GO:0055085 - transmembrane transport [Evidence
                     IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="MFS transporter"
                     /protein_id="HOQ52008.1"
                     /translation="MTAATPVTPATRARGGFCLAVTGTVVLMAAASAPSPFYPQLTEQ
                     LQLPPVATTLIFAIYAIPLLLALLTLGSLSDRVGRRGILTVGSVALSVSLLMFWAADS
                     FLLLLAARALQGLAAGLLVPVLNAMMVDHEPSRWLGAAALANTVAPMAGLGLGALTAA
                     GLLDLPGIDAGTIFLLLAAVFPLLAATAWTIPTPPVLPGDAGPDPRARRVSAATRRRI
                     IVSVAPAIIAGWVTNGLFLALGPGIIATRFHADTYLHQAGSIIILAAAGVVAATALQG
                     ATARRISVFGAVSLGAGTVLSLAALAVPTLPGYLASVAVVGAGFGTAFMGAMRTLLPH
                     VDPGQRATVMAVIYTISYLAMSVPVVLAGFLVPALTLPGAAAVLGGVVVLLSITATLT
                     HLRTPTELTPPATQHRPKGTRRP"
     gene            6125..7645
                     /locus_tag="PLF56_00175"
     CDS             6125..7645
                     /locus_tag="PLF56_00175"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_017882576.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="multicopper oxidase family protein"
                     /protein_id="HOQ52009.1"
                     /translation="MTAPAPRRSPHLTRRRFLALGATAAAVGALAACTPTPAWVSPTG
                     AAVDRTERGRGGTGRVVTATLTAAAATLDLAGTPASTFSYGSIPGPVLRVQAGDTLQA
                     TLRNTLPVDTSVHWHGLALRNDMDGVPPLTQTATRPGESFQYEFIAPDPGTHWFHPHV
                     GAQLDTGLYGVLIVEDPNEPLRYDDEWVIVLDDWLDGVTATPDAVLEQLREGMGDMGG
                     HGDMFMRMGNMLMGADSDLLGGDAGDVYYPHYLINGKPAADPAQFTGTPGTRVRIRLI
                     NAGGDTAFRVAIGGHRLTVTHTDGFPVDPVAADSVLVGMGERYDVIVTLGDGAFPLVA
                     EAEGKRERGFAIIRTGSGQAPTPDADIAELGEGRVVAVASMLFAAPEVTLDRRDVDRT
                     LDLTLTGSMANYDWGFNGTQFDMHNPLRGAHALVAGERVRLRVTNDTDMWHPFHLHGH
                     TYQHAGGGPRKDTSIVLPNQTLDLEFDADNPGQWMTHCHNIYHGEAGMMTVLAYRP"
     gene            7789..8001
                     /locus_tag="PLF56_00180"
     CDS             7789..8001
                     /locus_tag="PLF56_00180"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_019157651.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="YHS domain-containing protein"
                     /protein_id="HOQ52010.1"
                     /translation="MSHDHHHEAPAGTPADDIVTCPVIPGSTVSKSTAEAQGLYRDYD
                     GQRYWFCCSACGPLFDADPAKYATAA"
     gene            8064..9269
                     /locus_tag="PLF56_00185"
     CDS             8064..9269
                     /locus_tag="PLF56_00185"
                     /EC_number="1.-.-.-"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_006214163.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_function: GO:0000166 - nucleotide binding [Evidence
                     IEA];
                     GO_function: GO:0016491 - oxidoreductase activity
                     [Evidence IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="FAD/NAD(P)-binding oxidoreductase"
                     /protein_id="HOQ52011.1"
                     /translation="MSPQKVLILGAGAAGAAAARVLHGSGTVSVTLVDRTGQAPYNRT
                     LVNKGVAIGLLTPEQVVTPGVEVISDTAVEVDPQGKTVRLASGRIEPYDALLLATGST
                     PRLLPDTLPGLTAATEAGRVTTLHSLEDAVAVRDLLGTLGRPAQIVISGAGLVAAETA
                     TLLRDRGHQVTLLARATTPGVSVFGAGAAERLAAAHREHVDVAFGRTLTAVTDTGTQL
                     ELALDDTTQLTADLLIIAHGTTPTGPAPWEDGVIVDGKLRTATPGVYSAGGVAAHYDT
                     TLGAWRIDHWSDAAAQGEHAARTMLADLGAGTHPGDYQPRAPFTATVHGMMVAGAGIT
                     GGHTSARIDTTDPLLVVHEHDGVPVGVLGLDAVPAVFGWMPNLHTPAATDPQDLASAG
                     TATADSHQR"
     gene            9288..11462
                     /locus_tag="PLF56_00190"
     CDS             9288..11462
                     /locus_tag="PLF56_00190"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_020385353.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_function: GO:0042625 - ATPase-coupled ion transmembrane
                     transporter activity [Evidence IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="heavy metal translocating P-type ATPase"
                     /protein_id="HOQ52012.1"
                     /translation="MTDPHAHHTDHPTGKAATATGGDTRHGDHTPEPPTQHDTHTGHG
                     TQASHGDHAGHDGGGHGGGHGGHGDHVGQFRRLFWINLIIAVPVVAFSPMFAMILGYT
                     VPDWALFIAPVLGTVMYAWGGWPFLTGAVSELRSRKPGMMLLIGLAITVAFFASWGAT
                     LGLLHHELEFWWELALLIVIMLLGHWIEMRSLAQTSSALDTLAALLPDEAERVEGDQI
                     VKVSPADLQVGDVVVVRPGGSVPADGKIVDGRADMDESMVTGESRPVARGTGDPVTAG
                     TVATDSGLRVEVTATGDETTLAGIQRLVTEAQNSSSRAQRIADKAAALLFWFALGAAA
                     ITAVVWTVVGNPDAAVVRTITVLVIACPHALGLAIPLVVSIATERAARGGVLVKDRLA
                     LESMRTVDTVLFDKTGTLTKGEPTVSAVEPVGDLDADQVLAIAAAAEADSEHPLARAI
                     VRAAKDKNLTLPKATGFSSSPAVGVTATVDGAEIRVGGPRLLEDVGASEVDTADAWRS
                     EGAIILHVTRDGKVIGGLKLADEIRPESREAVDALHKLGVQVVMITGDAEAVANAVGA
                     ELGIDRVFAHVRPEDKSSKVAELQKEGRKVAMVGDGVNDAPALAQADVGIAIGAGTDV
                     AIASAGVILASSDPRSVLSVIQLSRAAYRKMKQNLWWAAGYNLISVPLAAGVLAPIGF
                     VLPMSIGAILMSLSTVVVALNAQLLRRIDLSPEASTRAALDS"
     gene            11481..11795
                     /locus_tag="PLF56_00195"
     CDS             11481..11795
                     /locus_tag="PLF56_00195"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_013865956.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="metal-sensitive transcriptional regulator"
                     /protein_id="HOQ52013.1"
                     /translation="MTTPAPDTAHAPHGYITDKDRYLNRLKRIEGQARGISKMVDEEK
                     YCIDILTQISALTSALQAVAVGLLDDHLKHCVLDAARMEGDQAEQKIKEASDAIARLV
                     RS"
     gene            11854..11924
                     /locus_tag="PLF56_00200"
     tRNA            11854..11924
                     /locus_tag="PLF56_00200"
                     /product="tRNA-Cys"
                     /inference="COORDINATES: profile:tRNAscan-SE:2.0.12"
                     /note="Derived by automated computational analysis using
                     gene prediction method: tRNAscan-SE."
                     /anticodon=(pos:11886..11888,aa:Cys,seq:gca)
     gene            complement(11954..12658)
                     /locus_tag="PLF56_00205"
     CDS             complement(11954..12658)
                     /locus_tag="PLF56_00205"
                     /inference="COORDINATES: ab initio
                     prediction:GeneMarkS-2+"
                     /note="Derived by automated computational analysis using
                     gene prediction method: GeneMarkS-2+."
                     /codon_start=1
                     /transl_table=11
                     /product="hypothetical protein"
                     /protein_id="HOQ52014.1"
                     /translation="MVVAPDTDQLLLGTHEGIYEVTDTGEWGGRVSTDSFDAMGLTAT
                     SQTLIASGHPGPGSPAEWGAPNLGVMRSTDAGVSWESVVFGGEKDFHALAAGADDTVY
                     AIATDVSDVIRSDDAGLTWEPTGADLVAASLAVDGRGRVIAATEEGVQISTDGGASFA
                     AWPEAPLLYTLGAAADRTQVVGVGTDGQIWVTGAGAASWEPAGRVDGRVQAVGMDAAG
                     RIVIVDETSGITILPG"
     gene            complement(12865..13476)
                     /locus_tag="PLF56_00210"
     CDS             complement(12865..13476)
                     /locus_tag="PLF56_00210"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_013864635.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="DUF305 domain-containing protein"
                     /protein_id="HOQ52015.1"
                     /translation="MMNKKMRLAAVAAVPLAAVLFLAGCADNGGSMPGMDHGGSSSAP
                     SESAEADFNMADSMFAMMMIPHHEQAVEMSDMILAKSDVDQRVLDLAQQIKDAQQPEI
                     ETMQGWLAEWGVSSDDMDMGEMEHGGGMMSDEDMAALESASGAEAAPLFLEQMIAHHQ
                     GAIEMAETELANGKNADALALAQAVVDTQSAEIETMQDLLGQL"
     gene            complement(13556..13981)
                     /locus_tag="PLF56_00215"
     CDS             complement(13556..13981)
                     /locus_tag="PLF56_00215"
                     /inference="COORDINATES: protein motif:HMM:NF040012.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="DUF6153 family protein"
                     /protein_id="HOQ52016.1"
                     /translation="MIGLSALRLRLPHPVRWLVAVCFAAAVIVGLMGMHTISSGHTAP
                     TTSENVEMPGGAPHHSSAPATGDEDAEACASCSPSGGHDVLMMMCVLALLAAALVLLV
                     PRAAWSSVWERHALRPPVTRCRPAPDRPPSLLELSISRT"
     gene            14093..14497
                     /locus_tag="PLF56_00220"
     CDS             14093..14497
                     /locus_tag="PLF56_00220"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_020385329.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="four-helix bundle copper-binding protein"
                     /protein_id="HOQ52017.1"
                     /translation="MTVTAQMFDTHPRANDIANRDALLACIDACLECGQACTTCADAC
                     LSEEMVADLTTCIRLNQDCADLCDTTARLLSRHTGTDTTVLRAALEALRAACAACAAE
                     CDTHADMHEHCRVCADACRRCEQACTNLLATL"
     gene            complement(14543..15484)
                     /locus_tag="PLF56_00225"
     CDS             complement(14543..15484)
                     /locus_tag="PLF56_00225"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_006214174.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_function: GO:0008324 - monoatomic cation transmembrane
                     transporter activity [Evidence IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="cation diffusion facilitator family transporter"
                     /protein_id="HOQ52018.1"
                     /translation="MGAGHNHGAPTTEAGAPGDHRARLWIAFGLTATIVIAQAIGSIV
                     TGSLALLTDTAHAITDASGLLVALIAATLMLRPANPKRTWGFRRIEVIAALGQAALLL
                     VVGTYAAVEGIRRLFEPPEVAAGEMLVFGIVGLAANVIAIVILSSSRGANFNMRAAFL
                     EVINDALGSLGVIVAAIVIATTGFQQADAIAGLLIAGLIVPRAIILMRDTTRVLMEFT
                     PAGLDLDEVRRHILELEHVTDVHDLHASTVATGLPTLTAHVVVEDECFTDGHAAEVLH
                     EVRECVAGHFPVSIQHSTFQIETAHLTEDEPALVRHR"
     gene            15578..16183
                     /locus_tag="PLF56_00230"
     CDS             15578..16183
                     /locus_tag="PLF56_00230"
                     /inference="COORDINATES: protein motif:HMM:NF014906.2"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_process: GO:0045892 - negative regulation of
                     DNA-templated transcription [Evidence IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="TetR/AcrR family transcriptional regulator
                     C-terminal domain-containing protein"
                     /protein_id="HOQ52019.1"
                     /translation="MADRLSRRDVIHAALALLDRGGADAITLRGIARHLDVHLNSVSL
                     QVGSKARLFDLMADAILGELSFDDLPHPPIDRVKEIFRRYRQVLLNHRDGAHLVSGTR
                     VSETNTLRLGEAVIAALLDAGVDPDLAVTTFWSLNYLVVGLVHEEQTTPEDGAHRFEQ
                     ELAAHSFPALTSVRDLFLDDNAAAHLEFGMDALLHRARHLD"
BASE COUNT         2659 a         6232 c         5025 g         2318 t
ORIGIN      
        1 gcccaccgga acgggcgagt caggcgagtt gagcttccag atcctcgacg ctgacgggac
       61 tgcgctgacc gcgtacgagt cctcgcatga gaaggacctg cacctgatcg tggtgcgcac
      121 cgatggaacc cagttccggc acgtgcaccc cacgctcgac gaggccaccg gcacctggac
      181 gacctcctgg gagtggaccg aagccggcag ctaccggatc tatgccgact tcgtgccgga
      241 cgtcgccgac ggccccgaca aggtcaccct gacccgcacg gtcgacgtcg cgggcgcgtt
      301 caccccgaac ccggcgaccg ccacgtcgac gacaagcgag gtcgacgggt acacggtcac
      361 cctcgatggg gacctgaccg cgggggagag ctccgagcta accttgtcgg tcacccgtga
      421 cgggcagcca gtcaccaccc tgcagccgta ccttggggcc ttcgggcacc tcgtcgcgct
      481 gcgcgatggg gacctggcct acctgcacgt gcacgccgaa ggcgacgacc cgcaggacgg
      541 cgacaccgcc ggacccgaca tccgattcgc ggccgaggcg cctaccaccg gccggtacct
      601 gctctacctc gacttccagg tcgacgggca ggtccacacc gccgagttcg tcgtggacgc
      661 cgggcacggc accggcgaca cggacacgga cgacacacac agcgacacgg gcgacggtca
      721 ctgaccgccc cgcggtgatc tgagagaaga aggaacacca tgagcaccac agcccccgca
      781 gaacagaccg cggactccgc cgtcgaactc cagatcggcg ggatgacctg cgcctcctgc
      841 gcgatgcgga tcgagaaaaa gctgaacaag ctcgacggcg tcgccgccac ggtcaactac
      901 gccaccgaga aggcgaaggt caccatcccc gccggatacg acccggccct gctgatcgcc
      961 gaggtcgaga agaccggcta cagcgcggcg cttccggccc cgaagaaggc caagaacgac
     1021 accgccgagg gcggggacgc ggacgatgcc gacccggagc tgaccagcct ccgcaatcgg
     1081 ctcatcgtct ccatcgtcct gagcgtcccg gtcatcgcga tggcgatggt ccccgccctg
     1141 cagttcacct actggcagtg ggcgtcgttg gcgctggcgg cgccggtcat cgtgtgggcg
     1201 gcgttcccgt tccacaaggc ggcgtgggcg aacctcaagc acggcaccgc gacgatggac
     1261 acgctcatct cgatgggcac gaccgccgcg ttcctgtggt cgctgtacgc gctgttcttc
     1321 ggcaccgccg gcacccccgg gatgacgcac ccgttcgagc tcaccctcgc cccctcggaa
     1381 ggagccgcga acatctacct cgaagtcgcc gccggggtga cgatgttcat cctcgccggc
     1441 cgctacttcg agaagcggtc caagcgtcag gccggcgccg cgctgcgcgc gctgctcgag
     1501 ctcggcgcga aggaagtatc cgtgctgcgc gacggggtgg agacgaagat cccggtcgag
     1561 gacctgcagg cgggggacga gttcatcgtc cgcccgggcg agaagatcgc caccgacggt
     1621 gtcgtggttt ccggcacgtc cgccgtggac gcctccatgc tcaccggcga atccgtcccg
     1681 gtcgaggtcg ccgaaggcga ccccgtcacc ggagccacgg tgaacgccgg cggacgcttg
     1741 gtagttcgcg ccactcgggt cggctcggac acgcagctgg cgcagatggc caagctcgtc
     1801 gaggacgccc agaccggaaa ggctgaggtg cagcgcctgg ccgacaagat ctccggcgtg
     1861 ttcgtcccga ttgcgatcct catcgccgtc gtcgcgatgg gggcatggtt gggtgccggg
     1921 ttccctgtcg ctgccgcgtt caccgccgcc gtcgccgtgc tggtcatcgc gtgcccgtgc
     1981 gccctgggtc ttgccacgcc gaccgcgctg ctggtcggca ccggccgcgg cgcgcagatg
     2041 ggcatcctga tcaagggccc ggaagtgctg gaatccaccc gcaaggtcga caccgtcgtg
     2101 ctcgataaga ccggcaccgt caccaccggc aagatgaccc tggtcaacgt cgtcaccgag
     2161 ccgggcaccg atgcggacga gctactccgc ctcgccggtg cactcgaaga cgcctccgag
     2221 caccccatcg cgcaggcgat cgcgaagggc gccacccagc agaccggcac cctccccacc
     2281 gtggaagggt tcacgaacct ggaaggcaag ggcgtgcagg gtgtcgtcga cggacacgca
     2341 ctcatcgtcg gccgggaatc gctgctggcc gagtggtcgc tgcgcctgag cgccgaggtt
     2401 gccgccgcga aggcgcaggc cgaaagcgaa ggcaagaccg tcgtcgccgt cggatgggat
     2461 ggtgccgctc gcggcatcct catcgtcgcc gacaccgtca agccgaccag caaggaggcc
     2521 atcgcccagc tcaaggcact cggcctgact ccggtgctgc tcaccggcga caacgaggcg
     2581 gtcgcccgcc agatcgccgc cgaggtcggt atcgaccagg tcatcgccga agtcctcccg
     2641 aaggacaagg tcgatgtcgt cacccgcctc caaggcgagg gaaaggtcgt cgcgatggtc
     2701 ggagacggtg tcaacgacgc ccccgcgctc gcgcaggccg acctcggctt ggcgatggga
     2761 accggcaccg acgtcgcgat cgaggcctcg gacatcaccc tggtccgcgg cgacctgcgc
     2821 agcgccgctg acgcgatccg gctgtcccgc aagaccctcg gcactatcaa gaccaacctg
     2881 ttctgggcct tcgcctacaa cacggccgcg atcccggtcg cagccctcgg tctcctcaac
     2941 ccgatgctcg ccggtgccgc catggcactg tccagcgtgt tcgtcgtggg caacagcctg
     3001 cgtctgcgcg gattcaagag catcgcccgc tgaccgggca acccggcagc acctaccacc
     3061 cacatcggaa ggaacaccac ccatgtccag ctgttgcagc accagcgccc ccgcccccgg
     3121 cggccgtgag aacctcctcg acgccgccgg tggcgccgac atgaccacct gccccgtgat
     3181 ggagggcacc ccggtcaaca aggccaccgc cgtggcggcc ggcctgttcc gcgactacga
     3241 gggcgagcgg tactacttct gctgcgccgg ctgcggtccc gcgttcgacg cggacccggc
     3301 ccgctacgcc gccgccatgg cctgagcctc actcccggtg cccacacacc ccgtgcgggc
     3361 accgggagac cggacccttc atgacctcca ccatcgcggc cgcccgcgaa ccgggctaca
     3421 tcaccgaccc ccgcacctac cggaaccggc tgcgccgtct ggaagggcag attcgcgggc
     3481 tcaccgggat gatcgagcag gaacgcccct gcatcgatac cctcacccag atctccgcca
     3541 cccaacgcgc gctccagggt gtcggtctcg ccctgctgac ggactacctg cgccacgcgc
     3601 acgccaccgg caccctcacc gacgacaccg tccttgatgc cgcctcgacc gcgatcaccc
     3661 agctgatccg gtcctgaccc tgccctcctc gcagcgccgc gacccccgta ccccggaagc
     3721 gaactgcacc tcatgcccac ccaccatgtc ctcatcctcg gggcaggagc agccggcgtc
     3781 gccgccgcaa cagccctcgc ccgccacgag gacatccacg tcaccctggt cggcagaacc
     3841 cacgagaccc cgtacatccg gatgcacatc accggcgtcg cgttcggtcc caccttgccc
     3901 gagaccatcc acgcgccgct cccgcccgtc accgtgctgg cagacaccgc cacccacctc
     3961 gacccgatca gccgcatcgt gcacctcgcc tccggcgcac agttgcgcta cgacgcggtc
     4021 atcatcgcca ccggcagccg cccggacacc ctcaccccgg gcctgcccgg gcggcacact
     4081 cccgctgcac ggcaccaggt cgtcaccctg cacagcatcc aggacgccca acagatccga
     4141 gaacacctca cccgcaccgc cgggccgtcc cgggtcgcga tctacggcgg cggattcacc
     4201 ggcgcggaga ccgcgtccgc actccacgcc gccggacatc acgtcgccct catcagccgc
     4261 tcccgcatcc ccgggcgggc ctccttcggc gcgcagatcg ccgcccacat caccggcatc
     4321 catcgcggca aaatcaccac attcctgggc cgcacgatca ccgccgtccg tgacaggagc
     4381 ccgctgctgg ggatcaccct ggatgacacc accacgctct ccgccgacct gctcatcacc
     4441 gccctcggca acgcccccac cgcaccggga ccgttctctt ccggggtgcc tgtcgatgac
     4501 cggctgcgga gcactgcccc gcacgtgtac gcggccggca gcaccgccat acaccgcgac
     4561 gaccacctcg ggatctggcg cctggatcac tgggccgacg cggccgcgca aggacagcac
     4621 gccgccgccg cggtgctcca cgaccgcacc ggcgccgccg acccgggacc ctaccgtcct
     4681 cgtagctcct acacgtcact gatccacggc accgccatca ccggcatcgg gtacaccatg
     4741 acgcccgcca ccgaaggaca gggcccagga cccggcaccc tcgtcacgca cacgctcaac
     4801 ggtgctctgg tgggggtgag cggtgtcgac gcggtcgcca ccgtccacga aaccgcccag
     4861 cacctgcacg agagcgccgc atgaccgccg ccacccctgt gaccccggcc actcgtgccc
     4921 gcggcgggtt ctgtctcgcc gtgaccggca ccgtcgtgct aatggcggcc gcgtcggcgc
     4981 cttccccgtt ctacccgcaa ctcaccgagc agctccagct gcccccggtg gccaccactc
     5041 ttatcttcgc gatctacgcc atcccgctgc tgctcgcact gctgacgctc gggtcgcttt
     5101 ctgaccgggt cgggcgtcgc ggcatcctca ccgtcggatc ggtggccctg tcggtgagcc
     5161 tgctgatgtt ctgggccgcc gattccttcc tgctgctgct cgccgcccgc gccctgcaag
     5221 gcctggcggc gggactgctg gtcccggttc tgaacgccat gatggtcgac cacgaaccct
     5281 cccgctggct gggggcggcc gcgctggcca acaccgtggc ccccatggcc ggtctcgggc
     5341 tcggggcgct caccgccgct ggactgctgg acctacccgg catcgacgcg ggcaccatct
     5401 ttctgctcct cgccgcggtg ttccctctcc tcgccgccac cgcctggacc attcccaccc
     5461 cacccgtcct tcccggggac gcgggaccgg acccacgggc ccggcgggtc tcggcggcga
     5521 cgcggcggcg gatcatcgtc tcggtcgccc ccgccatcat cgccgggtgg gtcaccaacg
     5581 gtctgttcct cgccctggga ccggggatca tcgccacccg gttccacgcc gacacctacc
     5641 tgcaccaggc cgggtccatc atcatcctcg ccgcggccgg ggtggtcgcc gccaccgcac
     5701 tgcagggggc caccgcccgg cggatcagcg tgttcggggc cgtgtctctc ggcgcaggca
     5761 ccgtgctcag cctcgccgcg ctggccgtgc caactctgcc cgggtacctc gccagtgtcg
     5821 cggtcgtggg tgccgggttc gggactgcct tcatgggcgc gatgcgcacc ctgctgccgc
     5881 acgtcgaccc cgggcagcgc gccaccgtta tggccgtgat ctacacgatc tcctacctcg
     5941 cgatgtccgt ccccgtcgtg ctggccggct tcctcgtccc cgctctcacc ctccctggag
     6001 ccgcggccgt gttgggggga gtggtggtgc tgctcagcat caccgccacc ctcacccacc
     6061 tgcgcacccc cacggagctc acaccgcccg ccacgcaaca tcgaccgaaa gggacccgcc
     6121 gaccatgacc gcccccgcgc cccgccgttc cccccacctc acccggcgac gcttcctcgc
     6181 cctcggcgcc accgccgccg cggttggagc tctggccgcc tgcaccccca cccccgcctg
     6241 ggtcagcccg acgggagcgg ccgtcgaccg caccgaacgg ggccgtggcg gcaccggccg
     6301 cgtggtcacc gcgacgctga cggcggcggc ggcgaccctc gatctggcag gaacacccgc
     6361 gtccacgttc tcctacggca gcatccccgg gcctgtcctg cgcgtccagg caggagacac
     6421 cctgcaggcc accctgcgca acaccctccc cgtcgacacg tccgtgcact ggcacggcct
     6481 ggcactccgc aacgacatgg acggcgtccc cccactcacc cagaccgcga cccgccccgg
     6541 cgagtcgttc caatacgagt tcatcgcccc cgaccccggt acgcactggt tccacccgca
     6601 cgtcggcgcc cagctggaca ccggcctata cggggtgctc atcgtcgaag acccgaatga
     6661 gccgctgcgc tacgacgacg aatgggtcat cgtcctcgac gactggctgg acggggtcac
     6721 cgccaccccc gacgcggtgc tcgagcagct gcgagaagga atgggcgaca tgggcgggca
     6781 cggcgacatg ttcatgcgga tggggaacat gctgatgggc gccgactccg acctactcgg
     6841 cggcgacgcc ggcgacgtgt actacccgca ctacctgatc aacggcaaac ccgccgccga
     6901 ccccgcccaa ttcaccggca cgcccggcac gcgggtacgg atccggctga tcaacgccgg
     6961 cggcgacacc gcgttccgcg tcgccatcgg cgggcaccgg ctgaccgtca cccacaccga
     7021 cgggttcccc gtcgacccgg tcgctgccga tagcgtcctg gtgggaatgg gggaacgcta
     7081 cgacgtgatc gtcaccctgg gcgacggtgc gttccccctg gtggcggagg ccgaaggcaa
     7141 gcgggaacgc ggcttcgcca tcatccgcac cggcagcgga caggcgccca ccccggacgc
     7201 ggacatcgcc gaactgggcg aaggccgcgt cgtcgcggtg gcgagcatgc tcttcgccgc
     7261 acccgaggtc accctcgacc gtcgtgacgt ggaccgcacc ctcgacctca ccctcaccgg
     7321 cagcatggcc aattacgact gggggttcaa cggcacccag ttcgacatgc acaacccgtt
     7381 gcgcggcgcg cacgcactgg tcgcggggga acgggtgcgg ctgcgggtga cgaacgacac
     7441 cgacatgtgg caccccttcc acctgcacgg ccacacctat cagcacgccg gtggcggccc
     7501 ccgcaaagac acctccatcg tgctgccgaa ccaaaccctc gacctcgagt tcgacgccga
     7561 caaccccggc caatggatga cccactgcca caacatctac cacggcgaag ccgggatgat
     7621 gaccgtgctc gcctaccgcc cctgagcacc agcagtccag ggaatacacg ccacacccca
     7681 cctgttgtca tacccgtaca gggtatagcc tggaggggtt cggacgcaga caaacgaggc
     7741 agcgacgacg gctgccgccc cggacccaca tcacggagag caacggatat gagccacgac
     7801 catcaccacg aagcacccgc cggcaccccc gccgatgaca tcgtcacctg cccggtgata
     7861 cccggcagca ccgtcagcaa gagcaccgcg gaagcccaag gcctctaccg ggactacgac
     7921 gggcagcggt actggttctg ctgctccgcc tgcggccccc tcttcgacgc cgaccccgcc
     7981 aagtacgcca cggccgccta accgaccaca ggccggggat accggtcacc agcccccaca
     8041 ttcgcgagac gcaaggagca acattgtcac cccagaaggt cctcatcctg ggagccggcg
     8101 ccgccggcgc ggccgcggcg cgcgttcttc acggttccgg cacggtgagc gtcaccctcg
     8161 tcgaccgcac cggacaggcc ccgtacaacc gcaccctggt caacaagggg gtcgcgatcg
     8221 ggctgctcac cccggaacag gtcgtcaccc ccggggtgga ggtcataagt gacaccgcgg
     8281 tcgaggtcga cccacaggga aagaccgtgc ggctcgcatc cggccggatc gaaccgtacg
     8341 acgcgctcct cctcgccacc ggcagcaccc cccgcttgct gccggacacg ctgcccggcc
     8401 tgaccgccgc cacggaggca ggacgggtca ccaccctgca ctccctggaa gacgcggtcg
     8461 ccgtccgcga cctcctgggc acgcttggcc gccccgcgca gatcgtcatc tccggtgccg
     8521 gtctggtcgc ggccgagacc gccacgctgc tccgcgaccg tggacatcag gtcaccctcc
     8581 tcgcccgcgc gaccaccccc ggggtcagcg tgttcggcgc cggcgccgcg gagcgcctcg
     8641 ccgccgcgca ccgggagcac gtggacgtcg cgttcggccg caccctcacc gccgtcactg
     8701 acaccggcac acagctcgag ctcgccttgg acgacaccac acagctgacg gcggatctgc
     8761 tgatcatcgc ccacggaacc accccgaccg gccccgcccc ttgggaagac ggggtcatcg
     8821 tggacgggaa gctgcgcacc gccacccccg gcgtctacag cgccggcggg gtcgccgcac
     8881 actatgacac caccctgggc gcctggcgca tcgatcactg gtcggacgcg gccgcgcagg
     8941 gtgaacatgc cgcacgcacc atgctcgccg acctcggcgc cggcacccac ccgggcgact
     9001 accagccccg cgcccccttc acggcaaccg tgcacggcat gatggtcgcc ggtgccggga
     9061 tcaccggggg tcacacctcc gcacgcatcg acactaccga cccgctcctg gtcgtccacg
     9121 agcatgacgg tgtccccgtc ggggtgctcg ggctggacgc cgtgcccgcg gtgttcgggt
     9181 ggatgccgaa ccttcatact cctgccgcga ccgatccgca ggacctcgcc tccgcgggaa
     9241 ccgcgacggc agactcacac caacgctagg cacagggaag tgaaccgatg actgacccgc
     9301 acgcacacca caccgaccac cccaccggca aggccgccac cgccaccggg ggagacaccc
     9361 gccacggcga ccacaccccc gagccgccca cccagcacga cacccacacc ggccacggca
     9421 cgcaggccag ccacggggac cacgcaggtc acgatggtgg tggccacggc ggcgggcacg
     9481 gtgggcacgg ggatcacgtc ggccagttcc ggcgactgtt ctggatcaac ctgatcatcg
     9541 ccgtcccggt cgtggcgttc tccccgatgt tcgcgatgat cctcggctac accgtccccg
     9601 actgggcact gttcatcgcc cccgtcctcg gcaccgtcat gtacgcgtgg ggcgggtggc
     9661 cgttcctgac cggtgcggtc agcgaactcc gctcccgcaa acccggcatg atgctgctga
     9721 tcggtctggc catcacggtc gcgttcttcg cgtcctgggg cgccaccctg ggcctgctgc
     9781 accacgagct ggagttctgg tgggagctgg cgctgctgat cgtgatcatg ctgctcggcc
     9841 actggatcga gatgcgctcc ctcgcccaga ccagctccgc gctggacacc ctcgcggcgc
     9901 tgctcccgga tgaggcagag cgcgtcgaag gggaccagat cgtcaaggtc tcacccgccg
     9961 acctgcaggt cggggacgtg gtggtggtcc gccccggcgg cagcgtcccc gccgacggga
    10021 agatcgtcga cggccgcgcc gacatggacg agtccatggt caccggtgaa tcccgccccg
    10081 tcgcccgcgg cactggcgac cccgttaccg cgggcaccgt cgccacagac tccggccttc
    10141 gcgtggaggt caccgccacg ggggatgaga ccaccctcgc gggtattcag cggctggtca
    10201 cggaggcaca gaactcgtcc tctcgggcgc agcggatcgc cgacaaggcg gcggcgctgc
    10261 tgttctggtt cgcgctgggc gccgccgcga tcaccgcggt ggtgtggact gtcgtcggca
    10321 atcccgatgc ggcggtcgtg cgcaccatca ccgtgctggt catcgcctgc ccgcacgcgc
    10381 tgggcctggc gatcccgctg gtggtgtcca tcgccactga gcgcgccgcc cgtggcgggg
    10441 tgttggtgaa ggaccggctc gcgctcgaga gcatgcgcac cgtcgacacc gtgctgttcg
    10501 acaagaccgg caccctcacc aagggcgagc ccaccgtctc cgccgtggaa ccggtcggtg
    10561 acctggacgc tgaccaggtg ctcgcgatcg ccgcggcggc cgaggccgac agtgagcacc
    10621 cgctcgctag ggccatcgtt cgcgccgcga aggacaagaa cctgaccctg ccgaaggcga
    10681 ccgggttttc ctcttccccg gcggtcggtg tcaccgcgac cgtcgacggt gcggagatcc
    10741 gtgtcggcgg tccccgtctg ctcgaggacg tcggtgccag cgaggtcgac acggcggacg
    10801 cgtggcgtag cgagggcgcg atcatcctgc acgtgacccg ggacggaaaa gtcatcggcg
    10861 ggctcaagct cgccgacgag atccgccccg agtcccgtga agccgtcgat gccctgcaca
    10921 agctgggtgt ccaggtggtc atgatcaccg gcgacgccga agccgtcgcg aacgccgtcg
    10981 gcgccgagct gggcatcgac cgggtcttcg cgcacgtgcg cccggaggac aagtccagca
    11041 aggtcgccga gctgcagaag gagggccgca aggtcgcgat ggtgggcgac ggcgtcaacg
    11101 acgcccccgc cctcgcgcag gccgacgtcg gcatcgcgat cggcgccggc accgacgtcg
    11161 cgatcgcgtc cgccggggtc atcctcgcga gctccgaccc gcgcagtgtg ctgtcggtga
    11221 tccagctgtc gcgtgcggcg taccggaaga tgaagcagaa tctgtggtgg gctgccgggt
    11281 acaacctgat ctccgtgccg ctcgccgccg gcgtgctcgc ccccatcggg ttcgtcctgc
    11341 cgatgtcgat cggtgcgatc ctgatgtccc tgtccaccgt cgtggtcgcc ctgaacgcgc
    11401 agctgctgcg ccgcatcgac ctcagccccg aagccagcac ccgcgcagcg ctggacagct
    11461 aaacccgaag gaacccggcc atgaccacac ccgcacccga caccgctcac gccccccacg
    11521 ggtacatcac cgacaaagac cgatacctga accggctcaa gcggatcgaa ggtcaagcgc
    11581 ggggcatctc gaagatggtc gatgaagaga agtactgcat cgacatcctc acccagatca
    11641 gcgctctgac cagcgccctg caggccgtgg ccgtcgggct gcttgacgac cacctgaaac
    11701 actgcgtgct cgacgcggcg cgcatggagg gtgaccaggc ggagcagaag atcaaggaag
    11761 ccagcgacgc gatcgcccgt ctggtccggt cctaaccgga ccagacgggc gatcccacgc
    11821 tgctgcgtcc acgtccccgg taaggggaca ctcggtgcct tggccgagcg gtcaggcaac
    11881 ggcctgcaaa gccgtctaga cgggttcgac tcccgtaggc acctcgcata cggcccgtcg
    11941 cgtgcccggc gggctatccg ggcagaatcg tgatgccgct ggtctcgtcg acgatcacga
    12001 tgcggcccgc ggcgtccata ccgaccgcct gcacccggcc gtccacgcga cctgccggct
    12061 cccagctcgc ggcaccggct ccggtcaccc aaatctgccc atcggtgccg acaccgacca
    12121 cctgagtccg gtcggccgca gcccccaacg tgtacagcag cggcgcttcg ggccacgccg
    12181 cgaaggacgc accgccgtcc gtgctgatct gcacaccctc ttcggtggcc gcgatcaccc
    12241 gtccgcgacc gtcgacggcg agactggccg ctaccagatc cgcaccggta ggttcccagg
    12301 tcagcccggc gtcgtcgctg cggatcacgt cgctgacgtc cgtggcgatg gcgtacaccg
    12361 tgtcatcggc gccggcggcg agggcgtgga agtctttctc cccaccgaac accaccgact
    12421 cccaactcac ccctgcgtcg gtgctgcgca tcacgcccag gttcggtgcc ccccactcgg
    12481 cggggcttcc ggggccgggg tgaccggacg cgatcagtgt ctgcgaagtg gcggtgagtc
    12541 ccatcgcgtc gaagctgtcc gtgctgaccc ggccacccca ctccccggtg tcggtgacct
    12601 cgtagatgcc ttcatgcgtt cccaggagaa gctggtcagt gtccggggcg acgaccaccg
    12661 cgtggacatg gctcggtacc ccggtgcgcc cgtccccagc cggcggcgac gtcggagtgt
    12721 ccgcgcaccc cgccagcatc aggagggcgg aagcagcggc gcccgccgcc agtgtcttcg
    12781 tatggacgtg catacgcgta cctccgcgtt cgaagagtgg gggcaccgcg catggcagcg
    12841 gcgcccccac tcgacggatc gggctcagag ctggccgagc agatcctgca tggtctcgat
    12901 ctccgcggac tgtgtatcga ccacggcttg cgcgagcgcc aacgcgtccg cgttcttccc
    12961 gttggcgagt tcggtctcgg ccatctcgat cgccccctgg tggtgggcga tcatctgctc
    13021 caggaacagg ggtgccgcct cggccccgga cgccgactcc agcgccgcca tgtcctcgtc
    13081 agacatcatc ccgccgccgt gctccatctc acccatgtcc atgtcgtcac tggacacgcc
    13141 ccactcggcg agccatccct gcatcgtctc gatctccggc tgctgggcgt ccttgatctg
    13201 ctgagccaga tccagcaccc gctgatcaac gtcgctcttg gcgaggatca tgtcgctcat
    13261 ctcgacggcc tgctcgtgat gcgggatcat catcatcgcg aacatcgaat ccgccatgtt
    13321 gaagtccgcc tccgccgatt ccgacggggc cgacgacgac ccaccatgat ccattccagg
    13381 catgctgcct ccgttgtccg cgcagccggc caggaacagc accgctgcca gcggtacggc
    13441 cgcgaccgct gccaaacgca tcttcttgtt catcatcagt cctctcaaac actcgtgaaa
    13501 cccgcgtacg ggcaatgaac ctcacccccg ccccagaggg cagggacagt gcgtgtcacg
    13561 ttcggctgat cgacagctcc aggagtgagg gaggccggtc cggggccggc cggcaccgcg
    13621 tcaccggcgg acgcaatgca tgccgctccc acacagacga ccatgcggcg cgggggacca
    13681 gcagcacgag ggcggctgcc agcaacgcca gcacgcacat catcatcaga acgtcatggc
    13741 ccccgctcgg gctgcacgac gcgcacgcct ccgcatcctc atcgccggtc gcgggcgccg
    13801 acgagtggtg aggtgccccg cccggcatct cgacattctc cgaggtcgta ggggcggtgt
    13861 gtcccgagga gatcgtgtgc atccccatca acccgacgat gaccgctgca gcgaagcaca
    13921 cggcaaccag ccaccgcacc gggtgcggca gccgcaggcg caacgcggac aggccgatca
    13981 tcccgtccag cctaccgtcc gtaccatacc cccgcaccgt aacccaaggg ggtcgccggc
    14041 atccggaaca gcgacgtaac gtaaccccct caccgattca aaggagaagg ccgtgaccgt
    14101 caccgcccaa atgttcgaca cccacccccg agccaacgac atcgccaacc gagacgcgct
    14161 cctggcctgt atcgacgcgt gcctcgagtg cggccaggct tgtacgacct gcgccgacgc
    14221 ctgtctcagc gaggagatgg tcgccgatct gaccacctgc atccgtctga accaggattg
    14281 cgccgacctc tgcgacacca ccgcgcgcct gctctctcgc cacaccggca cggacaccac
    14341 cgtgcttcgt gccgcgctgg aggcgttgcg cgcagcgtgc gccgcatgcg ccgccgagtg
    14401 cgacacccac gcggacatgc acgagcactg ccgggtctgc gccgacgcct gccgccgctg
    14461 tgaacaggcc tgcactaacc tgctcgcgac gctctgacac ccttcctccg ccgaccgcta
    14521 ccgcggcgct ggggggcccg gctcagcggt ggcggacaag ggctggctcg tcctcggtga
    14581 ggtgcgcggt ctcgatctgg aaggtcgagt gctgaatcga caccgggaag tgaccggcca
    14641 cgcattcgcg tacctcgtgc aacacctccg cggcatgccc gtcggtgaag cactcatcct
    14701 cgaccaccac atgcgcggtc agggtgggca ggccagtggc gaccgtcgat gcgtgcaggt
    14761 cgtgcacgtc ggtgacgtgt tcgagctcaa ggatgtgccg gcgtacctcg tccaagtcga
    14821 gcccggcagg ggtgaactcc atgagcaccc gggtggtgtc gcgcatcaaa atgatcgcgc
    14881 gcggcacgat cagtcccgcg atcaggagcc ctgcaatcgc gtcggcctgc tggaacccgg
    14941 tcgtggcgat cacaatcgcg gccacgatca ccccgagcga gccgagtgca tcgttgatca
    15001 cctccaggaa cgctgcccgc atattgaagt tcgcgccgcg gctcgaggag aggatcacga
    15061 tcgcgatcac attcgccgcg aggccgacga tgccgaacac gagcatctct cccgcggcga
    15121 cctccggcgg ctcgaacagg cgtcggatac cttcgacggc ggcgtaagtg cccaccacca
    15181 gcagcagtgc ggcctgcccg agtgcggcga tcacctcgat gcgtcggaat ccccaggtcc
    15241 gcttcggatt agccggccgc agcatcagcg tcgccgcgat cagcgcgacc agcagaccgg
    15301 acgcgtcggt gatcgcgtgt gcggtgtccg tcagcagcgc caggctgccg gtgacgatgg
    15361 aaccgatcgc ctgcgcgatc acgatcgtcg ccgtcaggcc gaacgcgatc cacagtcgtg
    15421 ctcggtgatc cccgggcgct cctgcctcgg tagtcggtgc accgtggttg tgacccgcac
    15481 ccatgtccca ctccttactt ggtcagtgac caatttcctt gatcggtgac caagctacta
    15541 cagtgttggt cactgaccaa gctcggagga gagacctgtg gccgaccgac tatcccgccg
    15601 cgacgtcatc cacgccgcct tggccctgct cgatcgcggc ggggcggacg ccatcaccct
    15661 ccgtgggatc gcccgacatc tcgacgttca cctcaactcg gtgtcgctgc aggtggggag
    15721 caaagcgcgc ctcttcgacc tgatggcaga cgccattctc ggcgagctct ccttcgacga
    15781 cctcccacac ccgccgatcg accgggtcaa agagatcttc cgccgctacc gccaggtcct
    15841 tctgaaccac cgcgacggcg cacatctcgt ctcgggtacg agggtctccg aaaccaacac
    15901 cctgcggcta ggggaggcgg tgattgccgc gctgctcgat gccggcgtcg accccgacct
    15961 cgccgtcacc accttctgga gcctcaacta cctcgtggtc ggactcgtcc acgaagaaca
    16021 gaccaccccc gaggacggcg cccaccgctt cgagcaggaa ctcgccgcac attcgttccc
    16081 cgcactcacc agcgtccgcg atctcttcct cgacgacaac gccgccgcac accttgaatt
    16141 cggcatggac gcgctcctgc accgcgcccg ccacctcgac tgagcatcat cgtcagcggc
    16201 gagccatccc tccatatgga agaatctgcg tatg
//
LOCUS       DAOSUG010000004        16186 bp    DNA     linear   ENV 10-JUN-2024
DEFINITION  MAG TPA_asm: Micropruina sp. isolate AMR_MDS_2195
            NODE_2214_length_16186_cov_4.031492, whole genome shotgun sequence.
ACCESSION   DAOSUG010000004 DAOSUG010000000
VERSION     DAOSUG010000004.1
DBLINK      BioProject: PRJNA925477
            BioSample: SAMN32864262
            Sequence Read Archive: SRR4236673
KEYWORDS    WGS; ENV; Metagenome Assembled Genome; MAG; Third Party Data; TPA;
            TPA:assembly.
SOURCE      Micropruina sp. (wastewater metagenome)
  ORGANISM  Micropruina sp.
            Bacteria; Bacillati; Actinomycetota; Actinomycetes;
            Propionibacteriales; Nocardioidaceae; Micropruina.
REFERENCE   1  (bases 1 to 16186)
  AUTHORS   Abdulkadir,N., Saraiva,J.P., Zhang,J., Stolte,S., Gillor,O.,
            Harms,H. and Rocha,U.
  TITLE     Genome-centric analyses of 165 metagenomes show that mobile genetic
            elements are crucial for the transmission of antimicrobial
            resistance genes to pathogens in activated sludge and wastewater
  JOURNAL   Microbiol Spectr 12 (3), e0291823 (2024)
   PUBMED   38289113
REFERENCE   2  (bases 1 to 16186)
  AUTHORS   Abdulkadir,N.
  TITLE     Direct Submission
  JOURNAL   Submitted (24-MAR-2023) Environmental Microbiology, Helmholtz
            Center for Environmental Research -UFZ, Permoserstr 15, Leipzig
            04318, Germany
COMMENT     The annotation was added by the NCBI Prokaryotic Genome Annotation
            Pipeline (PGAP). Information about PGAP can be found here:
            https://www.ncbi.nlm.nih.gov/genome/annotation_prok/
            
            ##Genome-Assembly-Data-START##
            Assembly Method        :: SPAdes v. 3.11.1
            Genome Representation  :: Full
            Expected Final Version :: Yes
            Genome Coverage        :: Not Applicable
            Sequencing Technology  :: Illumina MiSeq
            ##Genome-Assembly-Data-END##
            
            ##Genome-Annotation-Data-START##
            Annotation Provider               :: NCBI
            Annotation Date                   :: 05/04/2023 04:57:10
            Annotation Pipeline               :: NCBI Prokaryotic Genome
                                                 Annotation Pipeline (PGAP)
            Annotation Method                 :: Best-placed reference protein
                                                 set; GeneMarkS-2+
            Annotation Software revision      :: 6.5
            Features Annotated                :: Gene; CDS; rRNA; tRNA; ncRNA
            Genes (total)                     :: 2,776
            CDSs (total)                      :: 2,750
            Genes (coding)                    :: 2,714
            CDSs (with protein)               :: 2,714
            Genes (RNA)                       :: 26
            tRNAs                             :: 24
            ncRNAs                            :: 2
            Pseudo Genes (total)              :: 36
            CDSs (without protein)            :: 36
            Pseudo Genes (ambiguous residues) :: 0 of 36
            Pseudo Genes (frameshifted)       :: 2 of 36
            Pseudo Genes (incomplete)         :: 32 of 36
            Pseudo Genes (internal stop)      :: 2 of 36
            ##Genome-Annotation-Data-END##
FEATURES             Location/Qualifiers
     source          1..16186
                     /organism="Micropruina sp."
                     /mol_type="genomic DNA"
                     /submitter_seqid="NODE_2214_length_16186_cov_4.031492"
                     /isolate="AMR_MDS_2195"
                     /isolation_source="Activated sludge of municipal
                     wastewater treatment plants"
                     /db_xref="taxon:2737536"
                     /environmental_sample
                     /geo_loc_name="Canada"
                     /lat_lon="49.25 N 123.1 W"
                     /collection_date="2015-06-16"
                     /metagenome_source="wastewater metagenome"
                     /note="metagenomic"
     gene            complement(<1..730)
                     /locus_tag="PLF56_00235"
     CDS             complement(<1..730)
                     /locus_tag="PLF56_00235"
                     /inference="COORDINATES: protein motif:HMM:NF012514.2"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_function: GO:0016787 - hydrolase activity [Evidence
                     IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="NUDIX domain-containing protein"
                     /protein_id="HOQ52020.1"
                     /translation="MTIEPPAAGERRRPTGPRNPGDAWVVAPTGERYWGRFGAAGLLA
                     LDAERGVLLQHRVEWSHFGGTWALPGGARHEGESALDAALREAGEEAGVPSWAIRPRM
                     VSVLDLDIWSYTTVVGDVVTSFEAVIADPESLALAWVPPDEVAELPLHPGFAASWTKL
                     RPLLGVRPVVLVDAANVVGSVPDGWWNDRAGATRRLLARIESMVARGVAAGDLGLPAA
                     TWFPEWVVVVEGRASSVTGPPVVRL"
     gene            955..1485
                     /locus_tag="PLF56_00240"
     CDS             955..1485
                     /locus_tag="PLF56_00240"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_012918734.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="type 1 glutamine amidotransferase
                     domain-containing protein"
                     /protein_id="HOQ52021.1"
                     /translation="MSEQQKVAFLVSAEGIEQAELVEPWRAVEKAGHTPVLISPESGT
                     VQLFEHLDKADTQKVDVVLSDASLDDYAALVLPGGVANPDALRTDETAVNFVRDFMAS
                     GKPVAAICHAPWVLVEADVVRGKRLASWPSVQTDIRNAGGEWVDEEVVVDGNLISSRN
                     PDDIPAFNRELLAALK"
     assembly_gap    1578..1677
                     /estimated_length=100
                     /gap_type="within scaffold"
                     /linkage_evidence="paired-ends"
     gene            1971..3443
                     /locus_tag="PLF56_00245"
     CDS             1971..3443
                     /locus_tag="PLF56_00245"
                     /inference="COORDINATES: protein motif:HMM:NF023283.2"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="DUF3375 domain-containing protein"
                     /protein_id="HOQ52022.1"
                     /translation="MADAPAVTLAVALRRLHDEPASLLLRADNLPAVVAILHAQLGGT
                     QRVKAAHEFLQQLTDDLADLRGAGFDLPRTAAEYLGEWVRQGLIVRRPGDGRDETVEL
                     SPSAQQSVRFVTGLHSPRSSVTSSRLTNVLDMLERLSRDTDPAQESRLEVLHRERARL
                     DAEIEAVEAGRFEPLSDDLALERIAEVLRLAGEIPGDFAQVGADFETLNRELREQIIR
                     QAGSRGEVLDEVFSGVDLIEQSDAGRTFGAFFALVLNPDVAGALDQAVDAVLERGFAD
                     ALSSQETFLLRHLLTTLQSESTQVRAVMTGFSRSLRRFVETHEYREHRRLKGALDDAK
                     AIALRAAQHVKPLTPLQTSLDTSSFPMSSVGSWKLRNPADTRTAEPVQSHPSGVLDLQ
                     ALRLQVRESEIDFAELRAAIVDTLGHQPIATVGEVLDRHPATQGLASIVGLLVLAHSV
                     GSPATGHESVHWLSARQRARSVSVGRYLFTKVPSDWKETS"
     gene            3440..4117
                     /locus_tag="PLF56_00250"
     CDS             3440..4117
                     /locus_tag="PLF56_00250"
                     /inference="COORDINATES: protein motif:HMM:NF025205.2"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="DUF4194 domain-containing protein"
                     /protein_id="HOQ52023.1"
                     /translation="MTPTPRRVHPDVLPPVALADTATDDRHILTAADRGRLPELTRRV
                     LVSLLQGPYVARELHPNLWPVLVTNEDLIRERLGDLFLQLVSDQEAGVAFVRPMTDAD
                     VDLPRTIRSMPLTFVDTVLVLFLRERLLQGSGARVFVGRDEIDDHCAAFRAADDTNLA
                     LFGKRVNASVSKLKDASILRSTSEGDRYEVSPVLGLVFDADNVHAVAAELRRLAGEPA
                     DESHSES"
     gene            4114..7455
                     /locus_tag="PLF56_00255"
     CDS             4114..7455
                     /locus_tag="PLF56_00255"
                     /inference="COORDINATES: protein motif:HMM:NF024947.2"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="ATP-binding protein"
                     /protein_id="HOQ52024.1"
                     /translation="MTAQQRLGRIQLVNWGTFNGSFTLPVPRDGLLITGPSGSGKSSL
                     LDALAAVLVQPKWLMFNAAAQEGGVSDRNRSLISYVRGAFKREADDATGEVSTAYLRT
                     GATWSGIALTFDDGTGAGTSVIRLLHLPRGSNAADALSSLFVIADGDIDLLALAPFVE
                     NGLNIRQLRAAHPGWFTFQTYAGFASKLQRRLGIGSDQAQRLLHKTQSAKNLTSLDVL
                     LRDFMLDEPDTFTLANEAVTQFQELSQAHASVVDARRQVEVLAPLRGIDAELNELRQR
                     SDALLAQETHLRTVVVQLRAESATADLAAGERRVDALTHEQNRADAAVRDCADARERA
                     RSAVDGLGGREVRDLERTIEQYRAAEASVAHRRERWTGRCRQLGVPLPDSADGYVQFR
                     ERAEATRADLDESDQGRDQRYQLAARAAEAGTQVNALRGQLDALSRQKSNLDERLLLV
                     RQLLLESAEVPRQRLPFVGELIDVLPDQARWQGAIERVLRPFARTLLVPDDLYPYVSA
                     FVEGRHLGTRLVYEKLPAEPVASMQVGDSRSLVSKLTVADAEASGWVRAELARRFDYR
                     CVETVAELRGISRGVTLAGQVKHSISRHEKDDRSRIDDRSTWVLGTSTDAKRRLLESA
                     IGTAEETRSRTAQLRDDADRDRDDRLQRAGDLTRLLELTWTELDLDTPRSAVQRLSHE
                     LKQLLAGGTELAAARVSLQQADRALGTAQTRRDEIVGQLSQARAAVQSLQRDLAEWQR
                     ELAESATPPAPVREALVARLNTLDASLEKSQVLAQQQLAKEREDALRLRSRAVARAER
                     IMQTFKTDWPIPAQDWAIAVDFLADYLGRLELLQADRLPEFEDRFFTLLQTQSQNNIG
                     ILSTRLRNARREVRERIDPINASLRLTEYAPGRHLHVRVDDRRNPEVTDFLQNLSLIA
                     GGTLGETLGAEAGDTARVRAEQRFDRMQALLNRLASSDPSDVRWRQHCLDTRQHVKFL
                     AEVRDEAGQAVDYFAGAGGLSGGERQKLVIFCLAAALRYQLARDGAVTPSYGLVVLDE
                     AFDKTDPAFTKAGLEVFHTFGFQLLLATPLKMLQTLEDYVGGAAVVLNDSARGSRLEF
                     LDFHAGERVNEPLAEPAGEAQAALL"
     gene            7452..8588
                     /locus_tag="PLF56_00260"
     CDS             7452..8588
                     /locus_tag="PLF56_00260"
                     /inference="COORDINATES: protein motif:HMM:NF023223.2"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="DUF3322 domain-containing protein"
                     /protein_id="HOQ52025.1"
                     /translation="MRSPDQLRAWAHKKYAAHYRSWSGVADETPLVLTLGYPTDPPNE
                     AVAAADPAATTAWVQSWTVFERSAPPEVEVQWAIRQWAGFGSQRLPVRVVVRSPNAVA
                     SLAGQHRVWQTLVDRADLLHAAWPDRQRVAGALPGMAAKLVRLADADLPRLIATVNWF
                     LANPDSGLLARQVPVEGVDTKWLERHTDLVRRLVVALGGERALGLRVEPRRFRVRVFD
                     GGELTDFTAPAAELAALSWAPARVLLVENRDSMAPLSALPGVVAVHAQGLAAPELAVV
                     GWIAQAQILYWGDLDSHGMRILSLVRQVLPSTESLLMNRQTLTRFAALAGVEPAPFRG
                     EIGYLTPEEAAALRALRHGDLRLEQERIPWDHVVSTIAGALAYP"
     gene            complement(8874..9920)
                     /locus_tag="PLF56_00265"
     CDS             complement(8874..9920)
                     /locus_tag="PLF56_00265"
                     /inference="COORDINATES: protein motif:HMM:NF027219.2"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="putative zinc-binding metallopeptidase"
                     /protein_id="HOQ52026.1"
                     /translation="MRLLLCPECSAPLWLESLTCLQCGCEVMIDPVASSALPVSGTST
                     HHPCSNRNWQCNWAVPIEAEAASCYSCRLTRRSPDADDLQALSKLAVTGQSKRRLLIG
                     LAKLGLPVVPYWMREGGLAFDLLSSKSGDGPVIIGHANGVITINLAESLDDYRESMRV
                     RLDEPYRTMLGHFRHEVGHYYQWQLVEAPGGPLLDECRELFGDERASYRDAINRHYRE
                     GAPDDWRERHISSYATMHPWEDFAETFAHYQHILATLAIVARGGLVLSPQQSSVVAHE
                     VVPRDDYAGVAFSVALADWKWVAHLLNRANHAMGKGDLYPFTIPAPVAEKLDFVHRVV
                     QSSRVAEPLVGARG"
     gene            9960..10634
                     /locus_tag="PLF56_00270"
     CDS             9960..10634
                     /locus_tag="PLF56_00270"
                     /inference="COORDINATES: ab initio
                     prediction:GeneMarkS-2+"
                     /note="Derived by automated computational analysis using
                     gene prediction method: GeneMarkS-2+."
                     /codon_start=1
                     /transl_table=11
                     /product="hypothetical protein"
                     /protein_id="HOQ52027.1"
                     /translation="MDWWGIGLIVAAGVAVVVYGYVDDRRKTRERDAAMLAPPKRDIP
                     RFQPMAEAPAYLSELQARTRPDQLASTDLNALTRTDLLAAQAAAPSVTVGIAAKDFIT
                     DSPTGWAVAANPMVVVLSDEVSTVRELLPVLQRAQAAARPLVLVAPRFGAEVLSTLAA
                     NTVQGKQQCVPVPANVEQSERIAALTLARALSHTDLQAGWVPDDALGSIGTWISDRKT
                     SWLIAE"
     gene            complement(11549..11902)
                     /locus_tag="PLF56_00275"
     CDS             complement(11549..11902)
                     /locus_tag="PLF56_00275"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_011757717.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="metallopeptidase family protein"
                     /protein_id="HOQ52028.1"
                     /translation="MPVTMSDERFEQLVDRALDQIPPELAALVHNCVLVIEARAAAPD
                     GDLLGYYDGIPLSERDSQYGGVLPDRIVIFRDAILELCESEMEVVDEVRITVWHEVAH
                     YFGIDDERLHDLGYA"
     gene            12118..13308
                     /locus_tag="PLF56_00280"
     CDS             12118..13308
                     /locus_tag="PLF56_00280"
                     /inference="COORDINATES: protein motif:HMM:NF019844.2"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_function: GO:0005515 - protein binding [Evidence IEA];
                     GO_function: GO:0070064 - proline-rich region binding
                     [Evidence IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="SH3 domain-containing protein"
                     /protein_id="HOQ52029.1"
                     /translation="MPQHTRGLARSLTVIAVGLALSIGVGGPILVGPANAAPSETGAR
                     SGIAGTKVTTADLNLRSAPSLDAPIVRVIPKGTKVTTTGVMSGRFAQVTIGGVTNWLS
                     SLYLADVAAPLPAIAYRAVTTTTLALRATAAVDATSPGNLKPGTTVGLTGVHSVSYSQ
                     VVHHNAVAWVLTGYLNTKGQAAGVPVLPTATGRRYVAVDEVNVRATSATNGAVVASAT
                     QGTLLLITGKTANKRTEVIYNGAKRWAYTAYLSKTKPSASAPGTPGDGGSLGSASLDR
                     TNAYARAIVREIRAKFPQIKTVYGWRASSAYSSDHPAGRALDIMIPDYKSAAGKALGN
                     AIARYLQDNYARLHVHYLIWRQRQWNVERNTNPTTGWRGMADRGTDTDNHLNHVHVSV
                     YDVK"
     gene            13476..13551
                     /locus_tag="PLF56_00285"
     tRNA            13476..13551
                     /locus_tag="PLF56_00285"
                     /product="tRNA-Glu"
                     /inference="COORDINATES: profile:tRNAscan-SE:2.0.12"
                     /note="Derived by automated computational analysis using
                     gene prediction method: tRNAscan-SE."
                     /anticodon=(pos:13510..13512,aa:Glu,seq:ttc)
     regulatory      13648..13780
                     /regulatory_class="riboswitch"
                     /inference="COORDINATES: nucleotide
                     motif:Rfam:14.4:RF00174"
                     /inference="COORDINATES: profile:INFERNAL:1.1.4"
                     /note="cobalamin riboswitch; Derived by automated
                     computational analysis using gene prediction method:
                     cmsearch."
                     /bound_moiety="adenosylcobalamin"
                     /db_xref="RFAM:RF00174"
     gene            13852..14697
                     /locus_tag="PLF56_00290"
     CDS             13852..14697
                     /locus_tag="PLF56_00290"
                     /inference="COORDINATES: ab initio
                     prediction:GeneMarkS-2+"
                     /note="Derived by automated computational analysis using
                     gene prediction method: GeneMarkS-2+.
                     GO_function: GO:0003677 - DNA binding [Evidence IEA];
                     GO_process: GO:0015074 - DNA integration [Evidence IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="site-specific integrase"
                     /protein_id="HOQ52030.1"
                     /translation="MTNPAWLAPPDQSTAPTRLDALMHYWLGQRAGTVAAKTLRADQD
                     LLRLIPQAYLSRDAASIGPQDVMGILSGLRARGLSELSVRRHRSSLSQFFGWCFRNGL
                     IGQTPVDDTVGAVPVTPAVVNPFSADELEAAWVEWSEYSPVLADVMLVLARTGLRWSE
                     ARALSVADARPDQLRVTRSSSEGGDVRRLPASRVRVVPVVPRIRPIVHGLLTGRDPDE
                     LLLTTSFGSQLHRTAVLRRLNWSQTGRGRRLHDLRHTAAQLWLAEGVTAGNVRAWMGP
                     TRLAA"
     gene            complement(15190..>16186)
                     /locus_tag="PLF56_00295"
     CDS             complement(15190..>16186)
                     /locus_tag="PLF56_00295"
                     /EC_number="1.2.1.-"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_013160821.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=2
                     /transl_table=11
                     /product="aldehyde dehydrogenase"
                     /protein_id="HOQ52031.1"
                     /translation="AITPTTNPTETVINNSIGMLAAGNSVVFSPHPRATRITHWLIRE
                     LNRALVAAGAPANLIVTVQNPSIENTNAMMSHKLVRLLVATGGPGIVKAVLSSGKKAI
                     GAGAGNPPALVDATADIEKAARDIVLGSSFDNNLPCTSEKEVIAVDSITDLLKFEMVK
                     NGAYELRDPALIDKLVALVVNGRHAVTDWVGKDASVILEALGVLAPAGTKLIIAEVPF
                     EHPFVRVELMMPILPIVRAATLEQAMDWAVIAEHGNRHTAVMHSSDVNALTTMAKRIQ
                     TTIFVKNGPSYAGIGIGGEGYGTFTIAGPTGEGLTSARHFARRRRCVLVGGLNVR"
BASE COUNT         2596 a         5608 c         5356 g         2526 t
ORIGIN      
        1 ccaggcgcac aactggcgga ccggtcacgc tgctcgcacg tccctcgacc accaccaccc
       61 actcgggaaa ccaggtggcc gcgggcagcc cgagatcgcc cgctgcgaca ccccgtgcaa
      121 ccatgctctc gatccgtgcg agcagccgcc gggtggctcc ggcgcgatcg ttccaccagc
      181 catccggcac cgaaccgacg acgttggccg catccaccaa caccaccgga cgcaccccca
      241 gcagtggacg cagcttcgtc cacgaggcgg cgaaccccgg gtgcagcggg agctcagcaa
      301 cctcgtccgg cggcacccag gcgagggcca ggctctccgg atcggcgatc accgcctcga
      361 aggacgtcac cacgtcaccc acgacggtgg tgtagctcca gatgtccaga tcgagcacgc
      421 tgaccatgcg aggccggatc gcccacgacg gcactcccgc ctcctcgccc gcctcgcgca
      481 gggccgcatc gagcgccgac tccccctcgt gacgggcgcc gcccggcagc gcccaggtgc
      541 cgccgaagtg gctccactcc acccggtgct gcagcagcac gccgcgttcc gcgtctaggg
      601 ccaacaggcc ggccgcgccg aagcgtcccc agtagcgctc acccgtgggc gcgacgaccc
      661 aggcgtcccc ggggttccgc ggcccggtgg gacgtcgccg ctcaccagcc gccggcggtt
      721 cgatcgtcac cagaccaggc tcgcacgttc cgcgaacggg cgccccatcc ccgcgcacgc
      781 atgtcggtgg tctcaacagg gtcgaccgac gaaatggccc gatgggtgag gttgtcgaac
      841 gacggtgccg acacccggct caggggtgtg gagtagctcg accgacgagt cactccgacc
      901 cgtcccatcc ggcggggtgt tgtgtgatcc ggaccaccag cgcagggtgg agtgatgagt
      961 gaacaacaga aagttgcctt cctggtgtcc gccgagggaa tcgagcaggc cgaactcgtc
     1021 gagccgtgga gggccgtcga aaaggccggg cacacccccg tgctgatctc acccgaaagc
     1081 ggcaccgtgc agttgttcga gcacctggac aaggccgata cccagaaggt cgacgtcgtg
     1141 ctgtcggacg cgtccctgga cgactacgcc gcgctcgtcc tgcccggcgg cgtggccaat
     1201 ccggacgccc tgcgcaccga cgagacggcc gtgaacttcg tccgcgactt catggcctcg
     1261 ggtaagcccg tcgcggcgat ctgccacgcg ccctgggtgc tggtcgaggc ggacgtggtc
     1321 cgcggcaagc ggctcgcctc gtggcccagc gtgcagaccg acattcgcaa cgccggcggg
     1381 gaatgggtgg acgaagaggt ggtcgtcgac ggcaacctga tcagcagccg caaccccgac
     1441 gacattcccg cgttcaaccg cgagttgctc gccgccctga agtagccgac ccacagcctg
     1501 ggcggcccgg gtcagcggcc acgtcccttg gaccacaccc cctgaggtag ccgacccacc
     1561 gcctgggcgg cccgggtnnn nnnnnnnnnn nnnnnnnnnn nnnnnnnnnn nnnnnnnnnn
     1621 nnnnnnnnnn nnnnnnnnnn nnnnnnnnnn nnnnnnnnnn nnnnnnnnnn nnnnnnnacc
     1681 caccgcctgg gcggcccggg tcagcggcca cgtcccttgg accacacccc ctcagggagc
     1741 cgacccaccg cccgggcggc ccgggcagcc aggtttaccc cctggggcag gcggcttgag
     1801 gacggattgg ccgacgatgg cgcgacgacc agaggccgcc actcctgact ctcagcagcg
     1861 attgctcgct gcgatggcgc ttccgtcacc atcgtggtcc atcccccagc cgggctctgc
     1921 ttctgtcacc gccgctggtt acgctgacca gcgacgtaag gattaccgcc atggcagacg
     1981 cacccgcagt gacgctcgcg gttgccttgc ggcgcctgca tgacgagccg gcgtcgctgc
     2041 tgctgcgcgc cgacaacctg ccggcggtgg tcgcgattct gcatgctcag ctcggcggca
     2101 cccagcgcgt caaggccgcc catgagttcc tgcagcagct gaccgacgac ctcgccgatc
     2161 tgcgcggggc cgggttcgat ctgccgcgca ctgccgccga gtacctcggc gagtgggtgc
     2221 ggcagggcct gatcgtccgc cggccgggcg acggacgcga cgagacggtc gagttgtcac
     2281 cctccgcgca gcagtcggtt cgtttcgtga ccggcctgca cagcccccgc tcgtccgtca
     2341 catcgtcgcg actcaccaac gtgctcgaca tgctggagcg gctcagccgt gacaccgatc
     2401 cggcacagga gtcgcggctc gaggtgcttc accgtgagcg cgccaggctg gacgccgaga
     2461 tcgaagccgt cgaggcgggt cgcttcgagc cactgtcgga cgacctggcg ttggaacgca
     2521 tcgccgaagt gttgcggctg gccggcgaga ttcccggtga cttcgcgcag gtgggtgccg
     2581 atttcgagac cctcaaccgc gagttgcgcg agcagatcat tcgccaggcc ggctcacgcg
     2641 gtgaggtgct ggacgaggtg ttcagcggtg ttgacctgat cgaacagtcc gatgccggac
     2701 gcaccttcgg cgccttcttc gccctggtgc tgaacccgga cgtggcgggt gccctcgacc
     2761 aggccgtcga cgccgtgctc gagcggggtt tcgcggacgc cctgtcgagc caggagacgt
     2821 ttctgctgcg gcacctgttg accaccctgc agagcgaaag cacccaggtt cgggcggtga
     2881 tgaccggctt ctcacgcagc ctgcgtcgct tcgtcgagac ccatgaatac cgtgagcatc
     2941 gccgcctcaa gggtgccctt gacgatgcga aggccattgc gctgcgggcc gctcagcacg
     3001 tcaaaccgct cacgcctttg cagacgtcgc tcgacaccag ttcgtttccc atgtcgtccg
     3061 tcggctcgtg gaagctgcgc aacccggccg acacccgcac cgccgaaccg gtgcagagcc
     3121 atcccagcgg cgtgctcgat ctgcaagccc tgcgcctgca ggtgcgtgag agcgagatag
     3181 atttcgccga gttgcgggcc gcgatcgtcg acaccctggg tcaccagccg atcgccaccg
     3241 tcggcgaggt gctcgaccgg catccggcga cccaggggtt ggcgtcgatc gtgggtctgc
     3301 tggtcctggc ccactcggtc ggctcgcccg caaccggtca cgagagtgtc cattggctca
     3361 gcgcccggca gcgcgcacgt tctgtcagcg tcggccgcta ccttttcacc aaggttccca
     3421 gcgactggaa ggagacgtcg tgacccccac gccccgccgg gtgcatcctg acgttctgcc
     3481 gccggttgcg ctcgccgaca cggctaccga cgaccggcac attctgaccg ccgccgaccg
     3541 gggccggttg ccggaactga cccggcgggt tctggtcagc ctgttgcagg gcccctatgt
     3601 ggcccgcgaa ctgcacccga acctatggcc ggtgctggtg accaatgaag acctgatacg
     3661 cgagcgcctg ggcgacttgt ttctgcaact cgtttccgac caggaggcgg gcgtcgcgtt
     3721 cgtccggccg atgaccgacg ccgacgtcga cctgccgcgc accattcgca gcatgccgct
     3781 caccttcgtc gacacggtgc ttgtgttgtt tctgcgcgaa cggctgttgc agggctccgg
     3841 tgccagagtg ttcgtgggcc gtgacgagat cgacgatcac tgtgcggcgt tccgggccgc
     3901 cgatgacacc aacctggccc tgttcggcaa gcgggtcaac gcctcggtga gcaagctcaa
     3961 agacgcgtcc attctgcgca gcaccagtga aggcgaccgc tacgaggtgt cacccgtgct
     4021 cggcttggtg ttcgacgccg acaacgtgca cgcggtcgct gccgaactgc ggcgtcttgc
     4081 cggcgagccg gccgacgaga gccacagcga gtcatgaccg cacagcagcg gctcgggcgc
     4141 atccagttgg tcaactgggg caccttcaac ggctctttca ccctgcccgt gcctcgcgac
     4201 ggcctgctga tcaccggccc ctctggctcg ggcaagtcct ccctgctcga cgcgctggca
     4261 gcggtgctcg tgcagcccaa gtggctgatg ttcaacgcgg ccgcacaaga gggtggcgtg
     4321 tccgaccgca accgcagcct gatttcgtat gtgcggggcg ccttcaagcg tgaggcggac
     4381 gacgccacgg gcgaggtgtc caccgcgtac ctgcgcaccg gggccacctg gagcggcatc
     4441 gccctcacct tcgacgacgg caccggtgcc ggcaccagcg tgatccggct gctgcatctc
     4501 ccccgcggca gcaacgccgc cgacgcattg agttcgctgt tcgtcatcgc cgacggcgat
     4561 atcgacctgc tcgccctggc acccttcgtc gagaacggct tgaacattcg ccaactgcgg
     4621 gcggcgcatc cgggttggtt caccttccag acctacgccg gtttcgccag caaactgcag
     4681 cgccggctcg gaatcggctc tgatcaggcc caacggctgc tgcacaagac ccagtcggcg
     4741 aagaacctga ccagcctcga cgtgctactg cgcgacttca tgctcgacga gcccgacacg
     4801 ttcaccctgg ccaacgaggc agtcacccag tttcaggagc tgtcgcaggc ccacgccagc
     4861 gtggtcgatg cgcggcggca ggtcgaggtc ctggcgccgc tgcgcggtat cgacgccgag
     4921 ctgaacgaac tgcggcagcg cagcgacgca ctgctggcgc aagagacgca tctgcgcaca
     4981 gtcgtggtgc agctgcgcgc cgagtcggcg accgccgacc tggcagctgg cgaacggcgg
     5041 gtcgatgcgt tgacccacga gcagaatcga gccgacgccg ccgtccgaga ctgtgcagac
     5101 gccagggaac gtgcccgcag cgccgtggat ggactcggcg gacgcgaggt gcgcgacctc
     5161 gaacgcacca tcgagcagta ccgggccgcc gaggcgtccg tggctcaccg gcgcgaacgg
     5221 tggacggggc ggtgccggca actcggtgta ccgctacccg attccgccga cggctacgtc
     5281 cagttccgcg agcgagccga ggcgaccagg gccgacctgg acgagtccga tcagggtcgt
     5341 gaccagcgct accagttggc agcgcgggcg gccgaggccg gcacgcaggt gaacgcattg
     5401 cgcggccagc tcgacgcgct gtcacggcag aagtcgaacc tcgacgagcg gctgttgctg
     5461 gtgcgccaac tgttgctcga atccgccgag gtgccccgcc aacggctgcc cttcgtcggc
     5521 gagctgatcg acgtgctgcc cgaccaggcg cgctggcagg gcgccatcga gcgggtactg
     5581 cgtccgttcg cccgcacctt gctggtgccc gatgacctct acccctatgt gtcggccttc
     5641 gtcgagggca ggcatctggg cacgcgcctg gtgtatgaga agctgccggc cgaaccggtg
     5701 gcgtccatgc aggtgggaga ttcacgctcg ctggtgtcga agctgaccgt cgccgatgcc
     5761 gaagcgtccg gttgggtacg tgccgaactg gcccgccgat tcgattatcg gtgcgtcgaa
     5821 accgtggcag agcttcgcgg catcagccga ggcgtcaccc tggccggaca ggtcaagcac
     5881 tcgatcagcc ggcacgagaa agacgaccgc tcgcgcatcg atgaccgctc cacctgggtg
     5941 ctgggcacgt ccaccgacgc caagcggcgg ctgctcgagt cggccatcgg cacagccgaa
     6001 gagacgcggt cacgaaccgc ccaactgcgt gacgacgccg accgtgaccg agacgaccgc
     6061 ctgcagcgag ccggcgacct gacccgcctg ctcgaactca cgtggaccga gctcgacctc
     6121 gacaccccac gaagcgccgt gcagcggctc agccacgagt tgaagcagtt gctggccggc
     6181 ggcaccgaac tggccgcagc tcgggtgagc ctgcagcagg ccgatcgggc actgggcacg
     6241 gcgcagacaa ggcgcgatga gatcgtcgga cagctcagcc aagcacgggc cgccgtccag
     6301 agtctgcagc gcgatctggc cgagtggcag cgcgaacttg ctgaatcggc gacccccccg
     6361 gcaccggttc gcgaggcact ggtggctcgg ctgaacacgc ttgacgcctc gctggagaag
     6421 tcgcaggtgc tggcccagca acaactggcg aaagaacgcg aggacgcact gcgcttgcgc
     6481 agccgggcgg tcgcgcgcgc cgagcgcatc atgcagacgt tcaagaccga ctggccgatt
     6541 ccggcgcagg attgggcgat cgccgtcgac tttctcgccg actacctggg ccgcttggag
     6601 ttgctgcagg ctgaccggct gccggagttc gaggatcgct tcttcaccct gttgcagacc
     6661 caatcgcaga acaacatcgg catcttgtcc acgcggctgc gcaacgcccg ccgcgaggtg
     6721 cgcgaacgca tcgacccgat caacgcgtcc ctgcggctta ccgaatacgc gcccggccgt
     6781 cacctgcacg tgcgggtcga tgatcggcgc aaccccgagg tgaccgactt tctgcagaac
     6841 ctctcgctga tcgccggcgg cacgctcggt gaaaccctgg gcgccgaggc gggcgacacc
     6901 gccagggtgc gggccgaaca gcggttcgac cgcatgcaag cgctgctgaa ccggctcgcg
     6961 tcgtccgatc cgtccgatgt tcgctggcgt caacactgcc tcgacactcg ccagcacgtg
     7021 aagttcttgg cagaagtgcg cgatgaggcg ggccaagcgg tcgactattt cgccggcgcc
     7081 gggggcctgt ccggcggcga gcggcagaag ctcgtcatct tctgtctggc ggcggcactg
     7141 cgctaccaac tcgcacgcga cggggccgtc acgccgtcct atgggctggt ggtgctcgat
     7201 gaggccttcg acaagaccga ccccgcgttc accaaagcgg gtctcgaggt gtttcacacc
     7261 ttcggctttc agctgttgct ggccacgccg ctcaagatgc tgcaaaccct ggaggactac
     7321 gtggggggcg cggcggtcgt gctcaacgat tctgcgcgcg gctcacggct ggagtttctc
     7381 gacttccacg ccggcgaacg cgtgaacgag ccgctcgccg aacccgccgg tgaggcccag
     7441 gcagcgctgt tatgagaagc cccgaccaac tcagggcgtg ggcgcacaag aagtacgccg
     7501 cccactatcg ctcctggtcg ggcgtcgcgg acgagactcc gctcgttttg accctgggct
     7561 atccgaccga tccgcccaac gaggcggtgg cggccgcgga tcccgccgca accacggcct
     7621 gggtgcagtc gtggacggtc ttcgagcgat cggccccacc cgaggtcgag gtgcagtggg
     7681 caatacggca gtgggccggt ttcggtagcc agcggttacc ggtccgagta gtggtgcgca
     7741 gcccgaatgc cgttgcgtcg ttggccggcc agcaccgcgt gtggcagacc ctggtcgaca
     7801 gggcggatct gctgcacgcc gcctggcccg accggcagcg ggtcgcgggc gctttgccgg
     7861 gcatggccgc gaagctggtg cggcttgccg acgcagatct gccccgcctg atcgcaaccg
     7921 tcaactggtt tctggccaat cccgactcgg gcctgctggc ccggcaggtg ccggtcgaag
     7981 gagtggacac caagtggctc gagcggcaca ccgatctggt gcgccgcctg gtcgtcgcac
     8041 tcggcggcga acgggccctg gggctgcggg tcgagccgcg caggttccgc gtgcgggtct
     8101 tcgacggcgg cgaactcacc gacttcaccg ctcccgcggc agaactggcg gcactgtcgt
     8161 gggccccggc gcgcgtgctg ctggtcgaga atcgcgattc gatggcgccg ttgagcgcac
     8221 tgccgggcgt ggtggccgtg cacgcgcagg ggctggcggc ccccgaattg gcggtggtcg
     8281 gctggatcgc ccaggcccag attctgtact ggggcgatct ggacagtcac ggcatgcgca
     8341 ttctcagcct cgtccgtcag gtgctgccca gcaccgaaag cctgttgatg aacaggcaaa
     8401 cgctgacccg gttcgccgcg ctggcggggg tcgaaccggc gccattccga ggcgagatcg
     8461 gctatctcac gccggaggaa gccgccgcgc tgcgagcact gcggcatggc gacctgcggc
     8521 tggagcagga gcgcatcccg tgggatcacg tcgtcagcac catcgcgggc gcgctggcgt
     8581 acccatgaac agtaacctgt ggaccgggcc agagtggtca gcccgtcagt cgcccgatct
     8641 atctctggtc gagcgtgtcg tcaccctccc gagcagcgtt tccacacgct cgaccaaccg
     8701 acccgcattc gatcccttcg ccgccagcta tcgcgcactg aacagctcgt ccttcgcccg
     8761 acccaacccg gttgggcgcc gagcacccca cccgcgcccg cgcgtccggt gttgcggacg
     8821 tggtcgcggt cgacaccggc cgtccttcgt ggcgtgagaa ggtccagggc gccctagcct
     8881 cgagcgccca ccagcggctc tgccaccctg gaggactgca cgacccggtg tacaaaatcc
     8941 agcttctcgg ccacgggcgc gggaatggtg aacgggtaca ggtcgccctt gcccatcgcg
     9001 tgattggccc ggttcagcag atgcgccacc cacttccagt cggccagcgc gacactgaac
     9061 gccacgcccg cgtagtcgtc gcgcgggaca acctcatgag cgaccacgga cgattgctgc
     9121 gggctgagca ccaggccgcc acgcgccacg atggccaggg tggccagaat gtgctggtaa
     9181 tgggcgaagg tctcggcgaa gtcctcccag ggatgcatgg tggcgtacga cgaaatgtgg
     9241 cgctcacgcc agtcgtccgg ggcgccctcg cggtagtgcc ggttgatcgc atcgcggtat
     9301 gaggcgcgct cgtcgccgaa gagctcgcgg cattcgtcca gcagcggccc gcctggggcc
     9361 tcgaccagtt gccactggta gtagtggccg acctcatggc gaaagtgacc cagcatcgtg
     9421 cggtagggct cgtcgagccg tacccgcatg ctttcgcggt agtcgtccag tgactcggcg
     9481 aggttgatcg tgatcacccc gttggcgtgg ccgatgatca ccggcccgtc acccgacttg
     9541 gacgacagca ggtcgaacgc cagcccgccc tcacgcatcc agtagggcac caccggcagg
     9601 ccgagtttcg ccaggccgat caacagacgc cgcttggact gacccgtgac ggccagcttg
     9661 gacagcgcct gcagatcgtc cgcatccggg ctgcgccggg tcagccggca cgagtagcac
     9721 gaggcggcct cggcctcgat cggcacggcc cagttgcact gccagttgcg gttgctgcag
     9781 gggtggtgcg tgctggtgcc cgacaccggc agtgccgacg aggcgaccgg gtcgatcatc
     9841 acttcgcagc cgcattgaag gcaggtcagg ctttcaagcc acagcggggc ggagcactcg
     9901 gggcacagca gtagtcgcac gaatccaggc taccggcggc gaccgggcac actggcggta
     9961 tggactggtg gggcatcgga ttgatcgtgg ccgcaggagt cgccgtcgtc gtctacggct
    10021 acgtggacga ccgccgcaag actcgcgagc gcgacgcggc catgctggcg ccgccgaagc
    10081 gcgacattcc acgctttcag cccatggccg aggcgcccgc gtatctgagc gaactgcagg
    10141 cgcggacgcg tccggaccag ttggcgagca ccgacctgaa cgcactcacc cgcaccgatc
    10201 tcttggccgc gcaggcggcg gcacccagtg tgacggtggg catcgcggcc aaagacttca
    10261 tcacggactc ccccaccggc tgggcggtgg ccgcgaaccc catggtggtc gtgttgtccg
    10321 acgaggtgag cacggtgcgc gagctgctgc cggtgttgca gcgggcgcag gctgccgcgc
    10381 gtccgttggt gctggtggcg ccccgcttcg gcgccgaggt gttgtcgacc ctggccgcca
    10441 acaccgtgca gggcaagcaa cagtgcgtgc ccgtgccggc gaatgtggaa cagtcggagc
    10501 ggatcgccgc gctcaccctg gctcgggcgc tgtcgcacac cgacctgcag gccggctggg
    10561 tgccggacga tgcgctcggc agcatcggca cctggatcag cgaccgcaag accagctggc
    10621 tgatcgccga gtaggcgggt tcggaggggc cgggcgctgg tttgtggccg gcgggtcggg
    10681 cgctgcctct tgcccggaag cctggcgctg cttcgtatcc ggtggggtcg ggcgctgcct
    10741 tttggccggc ggccgggcgc tgcctcgtgt ccggcaggct gggcgctgcc tcgtgtccgg
    10801 cggccgggcg ctgccttgtg tcccgcggcc gggcgttgcc tcttgttcgg cggccgggca
    10861 ttgcctcttg cccggccggc cgggcgttgc ctcttgtccg gcaggccgga cgctgcccct
    10921 ggcagtccct tatcaagcgg gacgcctgaa aagcctgcac cagtgagcct gctcacggcg
    10981 cgctgagcgg agggcctact cactggtgca gggatttcag gcacccccgg taccgcagtt
    11041 gagaaccgtg gcaccggcag acaccggcag gcggagtttg agtgcacgca ggcctgcccg
    11101 gctcgtcctg actaccctcg cgacacggaa ctgccccggc gcaccgaccc tctcgtcccg
    11161 actgctccac gacaggtagc tgccgcgcgc aggcacctgc gtgtcacgaa gcccgaatcc
    11221 accgatgtcc cggctgcgcg accccgtccg ggcgccctgg gcgtcgccgg ccacgctcac
    11281 cagatgcccg gcaccttcgc tcggaccggc ttgccaggcc acccgtctga taccgctcgc
    11341 gacgccggcc atcggtggat ccgggctaga gcgggctaca gcgggctaca gcgggctaga
    11401 gcgggctaga gcgggctaga gcagctacgt cggaggacgt ggcacgaagg cggctgcccc
    11461 tacgacagtg aaacgttcga gatctctcaa cccacactca aggtgtcttc aggctggcgc
    11521 gggcggcgac aaacccgccg tggcgacctc aggcgtagcc caggtcgtgc agtcgctcat
    11581 cgtcgatgcc gaagtagtgg gccacctcgt gccacaccgt gatgcgcacc tcatcgacca
    11641 cctccatctc gctctcgcac aactcgagaa tcgcgtcgcg gaagatgacg atccggtccg
    11701 gcagcacgcc gccatactgc gaatcacgct ccgacaaggg gataccgtcg taatagccga
    11761 gcagatcccc atcgggagca gcagcccgtg cctcgatcac caacacgcag ttgtgcacca
    11821 gtgcggccaa ctcaggcgga atctgatcga gcgctcgatc gaccaactgc tcaaaccgct
    11881 cgtcgctcat cgtcaccggc attgagccac tctagccagc acgcatgcga agaatgacat
    11941 aactgtggtt tctcagctgc ttgcggcgtg gcgtcttgca tctcccgcag aaaccacttt
    12001 ctccattgtg cgagccggtc cagcgtccct agagtccagc caacctgaga cgcagatgag
    12061 aacgcacgtg ccactgatgg cctcgggatt cgatgcacga gggagtgatc cagaaccatg
    12121 ccgcagcaca cgagaggcct ggcccgcagc ctcaccgtga tcgcggtcgg tctggcgctc
    12181 agcatcggcg tgggtggccc gatcctggtg ggcccggcga acgcagcacc gtccgagacc
    12241 ggcgcgcgca gcggcatcgc cggcacgaag gtgaccaccg cggacctgaa cctgcgcagc
    12301 gcccccagcc tggacgcacc gatcgtgcgc gtgatcccca agggcaccaa ggtcaccacg
    12361 accggggtga tgtccggacg attcgcgcag gtcaccatcg gcggcgtcac caactggctc
    12421 tccagcctct acctggccga tgtcgccgct cccctgccag cgatcgcata ccgggcggtc
    12481 accacgacca cgctcgcgct gcgtgcaact gcggcggtcg atgcgacctc tcccgggaat
    12541 ctaaagcccg gcacgacggt gggcctgacc ggggtccact cggtcagcta ctcccaggtg
    12601 gttcaccaca acgcggtcgc ctgggtgctc accggctacc tgaacaccaa gggccaggcc
    12661 gccggcgttc ccgtgctgcc caccgccacc ggacgtcgct acgtcgcggt ggacgaggtg
    12721 aacgtgcgcg ccacgtccgc cacgaacggc gcggtggtcg cgtctgccac gcagggcacg
    12781 ctgctgttga tcaccggcaa gaccgccaac aagcgcaccg aggtgatcta caacggcgcc
    12841 aagcgctggg cgtacacggc gtacctgagc aagaccaagc cgagtgcatc cgccccgggc
    12901 acaccgggcg acggcggcag cctgggcagc gccagcctgg accgcaccaa cgcctacgca
    12961 cgcgcgatcg tccgcgagat cagggcgaag tttccccaga tcaagaccgt ctacggttgg
    13021 cgcgcgagca gtgcgtactc gtccgatcac ccggccggac gggcgctcga catcatgatt
    13081 cccgactaca agtcggccgc cgggaaggcc cttggcaacg ccattgcgcg ctacctgcaa
    13141 gacaactacg cgcggctgca cgtgcactac ctgatctggc gtcagcggca gtggaacgtg
    13201 gagcgcaaca ccaacccgac caccggctgg cgcggcatgg ccgatcgtgg caccgacacg
    13261 gacaaccacc tgaaccatgt gcacgtgtcg gtgtacgacg tgaagtagcg ggacggctcg
    13321 ggcggacgcg tcgctacccg ccacgggcac cggcgtgggt tggcgagccg tcctgggcac
    13381 ggcgacggtc gccgcgtccg ctggctcctg tggcaccaca cgcactcgct gcgtttgggc
    13441 ccgcacgccc cggcccggta tggtttatcg gtcaggtccc catcgtctag cggcctagga
    13501 caccgccctt tcaaggcggc agcgcgggtt cgaatcccgt tggggatgcc atcaggaacg
    13561 ccactcaggg cttggaccgc gggatggtcg ttgtccacag ctttgagcct cgccgagaga
    13621 tgtcacattc gcgttctcaa ccaccacttc tcgctccggg gtagtagtgt gacccatacc
    13681 gccacgcgga ggaaagccgg tgaaaatccg gcactgacgc gcaaccgtga gccgaccatt
    13741 gtggtcgacg agccggatca ctcctcgtgg gggcagaacc tttacccgct gtcgcgaatg
    13801 catcgggggc ggcgcatccg tctcctttgc tgcagcagaa ggaagacgag catgacgaac
    13861 cccgcgtggc tggctccccc agaccaatcc accgccccca cccggttgga cgccctgatg
    13921 cactactggc tggggcaacg cgccggcacg gtggcggcga agacactgcg tgccgaccag
    13981 gacctgctcc gactcattcc gcaggcttat ctcagccggg acgcagcctc cattggtccg
    14041 caggacgtga tgggcattct cagcgggctg cgcgcccggg ggctgtcgga gttgtcggtg
    14101 cgccgccacc gctccagcct gtcgcagttc ttcggctggt gcttccgcaa cgggctgatc
    14161 ggccagaccc cggtcgatga caccgtcggg gccgtgccgg tcacgcccgc cgtggtgaac
    14221 cccttcagcg cggacgaact cgaagcggcc tgggtggaat ggtccgagta ctcgccggtg
    14281 cttgccgatg tgatgctggt gctggcccgc accgggctgc gctggagcga ggctcgcgcc
    14341 ctgtccgtcg cggacgctcg gcccgatcag ctgcgcgtga cccggtcgag cagcgaaggt
    14401 ggcgacgtgc gacggctgcc cgcaagccgt gtgcgcgttg tgccagtggt gccgcgaatc
    14461 cgtccgatcg tgcatggact gctgaccggc cgtgaccccg atgaactgtt gttgacgacg
    14521 agtttcgggt cgcagttgca ccgcacagcg gtcctgcgtc gcttgaactg gtcccagacc
    14581 gggcgcggcc gacggctgca tgacctgcgc cacacggcgg cccaactgtg gctggccgag
    14641 ggtgtcaccg ccgggaacgt ccgggcgtgg atgggtccga cccgcctggc cgcatagtca
    14701 gccgggcgtg agggacgccg gccatgattg cgacaggccg ctaccggcca ggacctgtgg
    14761 cgagggtcag cgctctgcgt cgagtgattc tgaaatgacc cgcaggggat cgcaccactc
    14821 acggcgtgtt gcgggtcgag gcagtgactg gtgtcgggat ttcagaaagt gacgaaggca
    14881 aatgcgcgaa gagtgcttcg cgggcgggcg cgcgccaagg tgacggccgg gtcggtttcg
    14941 cgactccccc ctcaatcgat ccgaaccctt gctctggatg ggcttctgag atcctgacac
    15001 cagtcactgc cccgacccgc aacacgccgg gcttctgggg aactacggcg ggtgatttca
    15061 gaatcactca accgagagtc gccgaggctc ccccatcagt ctctcgcttc atacgaacac
    15121 cacgatggca ggcggggcca ccgcgcaccc gcgaccgcaa cggccggaag gaaccgtgcc
    15181 cgatgcgggt cagcgcacat tcagcccgcc caccagcacg cagcggcggc gccgggcgaa
    15241 atgcctggcc gaggtcaggc cctcgccggt gggtccggcg atggtgaacg tgccgtaacc
    15301 ctcgccaccg atgccgatgc ccgcgtatga ggggccgttc ttgacgaaga tcgtggtctg
    15361 gatgcgtttg gccatcgtgg tcaacgcgtt cacgtcggag gagtgcatca ccgccgtgtg
    15421 ccggttgccg tgctcagcga tcaccgccca atccatcgcc tgctccagcg tcgccgcccg
    15481 cacaatcggc aggatcggca tcatcaactc cacccgcacg aacggatgtt cgaacggcac
    15541 ctcggcgatg atcagcttcg tacctgccgg ggcgagcacc ccgagcgcct ccagaatcac
    15601 cgacgcatcc ttgcccaccc agtcggtgac ggcgtgtcgt ccgttgacca ccagcgcgac
    15661 cagcttgtcg atcagggccg ggtcgcgcag ctcgtacgca ccgttcttga ccatctcgaa
    15721 cttgagcagg tcggtgatcg aatcgaccgc gatcacctcc ttctcagagg tgcagggcag
    15781 gttgttgtcg aagctcgacc ccagcacgat gtcgcgggcg gccttctcga tgtcggcggt
    15841 cgcgtcgacc agcgcaggtg ggttaccagc acctgcgccg atcgccttct ttccgctgct
    15901 gagtacggct ttcactatgc cggggccgcc ggtggcgacc agcagtcgca ccagcttgtg
    15961 gctcatcatc gcgttggtgt tctcgatcga ggggttctgc acggtcacga tcaggttggc
    16021 cggcgcgccc gccgcgacca gcgcccggtt cagctcgcgg atcagccaat gcgtgatcct
    16081 ggtggcccgc gggtgcgggc tgaacaccac cgagttgccc gcggccagca tgccgatcga
    16141 gttgttgatc acggtctcgg tcgggttcgt ggtgggcgtg atcgcg
//
LOCUS       DAOSUG010000005        15452 bp    DNA     linear   ENV 10-JUN-2024
DEFINITION  MAG TPA_asm: Micropruina sp. isolate AMR_MDS_2195
            NODE_2338_length_15452_cov_4.465610, whole genome shotgun sequence.
ACCESSION   DAOSUG010000005 DAOSUG010000000
VERSION     DAOSUG010000005.1
DBLINK      BioProject: PRJNA925477
            BioSample: SAMN32864262
            Sequence Read Archive: SRR4236673
KEYWORDS    WGS; ENV; Metagenome Assembled Genome; MAG; Third Party Data; TPA;
            TPA:assembly.
SOURCE      Micropruina sp. (wastewater metagenome)
  ORGANISM  Micropruina sp.
            Bacteria; Bacillati; Actinomycetota; Actinomycetes;
            Propionibacteriales; Nocardioidaceae; Micropruina.
REFERENCE   1  (bases 1 to 15452)
  AUTHORS   Abdulkadir,N., Saraiva,J.P., Zhang,J., Stolte,S., Gillor,O.,
            Harms,H. and Rocha,U.
  TITLE     Genome-centric analyses of 165 metagenomes show that mobile genetic
            elements are crucial for the transmission of antimicrobial
            resistance genes to pathogens in activated sludge and wastewater
  JOURNAL   Microbiol Spectr 12 (3), e0291823 (2024)
   PUBMED   38289113
REFERENCE   2  (bases 1 to 15452)
  AUTHORS   Abdulkadir,N.
  TITLE     Direct Submission
  JOURNAL   Submitted (24-MAR-2023) Environmental Microbiology, Helmholtz
            Center for Environmental Research -UFZ, Permoserstr 15, Leipzig
            04318, Germany
COMMENT     The annotation was added by the NCBI Prokaryotic Genome Annotation
            Pipeline (PGAP). Information about PGAP can be found here:
            https://www.ncbi.nlm.nih.gov/genome/annotation_prok/
            
            ##Genome-Assembly-Data-START##
            Assembly Method        :: SPAdes v. 3.11.1
            Genome Representation  :: Full
            Expected Final Version :: Yes
            Genome Coverage        :: Not Applicable
            Sequencing Technology  :: Illumina MiSeq
            ##Genome-Assembly-Data-END##
            
            ##Genome-Annotation-Data-START##
            Annotation Provider               :: NCBI
            Annotation Date                   :: 05/04/2023 04:57:10
            Annotation Pipeline               :: NCBI Prokaryotic Genome
                                                 Annotation Pipeline (PGAP)
            Annotation Method                 :: Best-placed reference protein
                                                 set; GeneMarkS-2+
            Annotation Software revision      :: 6.5
            Features Annotated                :: Gene; CDS; rRNA; tRNA; ncRNA
            Genes (total)                     :: 2,776
            CDSs (total)                      :: 2,750
            Genes (coding)                    :: 2,714
            CDSs (with protein)               :: 2,714
            Genes (RNA)                       :: 26
            tRNAs                             :: 24
            ncRNAs                            :: 2
            Pseudo Genes (total)              :: 36
            CDSs (without protein)            :: 36
            Pseudo Genes (ambiguous residues) :: 0 of 36
            Pseudo Genes (frameshifted)       :: 2 of 36
            Pseudo Genes (incomplete)         :: 32 of 36
            Pseudo Genes (internal stop)      :: 2 of 36
            ##Genome-Annotation-Data-END##
FEATURES             Location/Qualifiers
     source          1..15452
                     /organism="Micropruina sp."
                     /mol_type="genomic DNA"
                     /submitter_seqid="NODE_2338_length_15452_cov_4.465610"
                     /isolate="AMR_MDS_2195"
                     /isolation_source="Activated sludge of municipal
                     wastewater treatment plants"
                     /db_xref="taxon:2737536"
                     /environmental_sample
                     /geo_loc_name="Canada"
                     /lat_lon="49.25 N 123.1 W"
                     /collection_date="2015-06-16"
                     /metagenome_source="wastewater metagenome"
                     /note="metagenomic"
     gene            3..992
                     /locus_tag="PLF56_00300"
     CDS             3..992
                     /locus_tag="PLF56_00300"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_013864471.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_function: GO:0042626 - ATPase-coupled transmembrane
                     transporter activity [Evidence IEA];
                     GO_function: GO:0140359 - ABC-type transporter activity
                     [Evidence IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="ABC transporter permease"
                     /protein_id="HOQ52032.1"
                     /translation="MNPDEPRVDDPVVPDITVATSVGLIERPPLLAEAMGDDEIDEEG
                     TSLWKGAFQRLIRNPSAILGAVIVTLFVLVAILAPVLAPYEPAGSQWINQITPSTLPP
                     FSPEHPLGLDPFGSDLLTQLIFGARQSLIIGVVSTLLGLIGGAILGGLAGAFGGWVDV
                     AVMRLVDILLSIPSLLLAVSVAAVMGRQPLAIMVAIAAAQVPIFARLLRGSMLAESRK
                     DYVLASWSLGLRRRTITMGHVLPNSLGPVLVQATLSLATAIIEVAALSYLGLGDPDPA
                     VAEWGRMLVKAQDRLQSAPHLTLFPGICIAVTALGFTLLGEALREALDPKSRR"
     gene            995..1978
                     /locus_tag="PLF56_00305"
     CDS             995..1978
                     /locus_tag="PLF56_00305"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_013864470.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_function: GO:0005524 - ATP binding [Evidence IEA];
                     GO_function: GO:0016887 - ATP hydrolysis activity
                     [Evidence IEA];
                     GO_function: GO:0042626 - ATPase-coupled transmembrane
                     transporter activity [Evidence IEA];
                     GO_function: GO:0140359 - ABC-type transporter activity
                     [Evidence IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="ABC transporter ATP-binding protein"
                     /protein_id="HOQ52033.1"
                     /translation="MREPLLEIDNLSVTFRRRRSVTRAVDGVSYSVAPGEIVGVVGES
                     GSGKSVTVLALMGLLPDRGVEVTGQVRYAGRDLLSLGDSAMAKLRGREIAMVFQDPIS
                     SLNPVVPIGVQVAEVLLTHTRTTRRAAMAESVDLLRRCGIPDPERRLKEYPHQLSGGM
                     RQRALIAMALACKPKLLICDEPTTALDVTIQAQVLELLKELVTDSGTAMIMITHDLGV
                     VAGLCDQVNVMYSGRIVEAAPRTELFAHPRHRYTHGLLASIPRLDLPRGEPLHPIPGT
                     PRDTVAWAKACAFAPRCRFATDACVTPDIALAPLSDSPPDHPVRCVHPAEA"
     gene            1980..3068
                     /locus_tag="PLF56_00310"
     CDS             1980..3068
                     /locus_tag="PLF56_00310"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_002528175.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_function: GO:0005524 - ATP binding [Evidence IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="ATP-binding cassette domain-containing protein"
                     /protein_id="HOQ52034.1"
                     /translation="MVEPVDTEPGRDDLLVVENLQVHFPIRKGLVFDRTVGHVKAVDG
                     VSLRVPRGSTYGLVGESGCGKSTFGRAVLRLEEPTGGTVTLDGESVTGLKGENLRQAR
                     RRMQMVFQDPLASLDPRQSVQSLMIEPLRVHQPAPHEQLVARAAEMLELVGMPRSALA
                     KYPHEFSGGQRQRIGIARAMILNPELVIADEPVSALDVSVQAQVINLLEKLQHEFGLT
                     YIVIAHDLAVVRHISDVVGVMYLGRLVEEAPSDALYAQALHPYTIALMSAIPIPDPEV
                     EDRRERILLRGDLPSPANPPSGCRFNTRCPYRQPTRCDTDEPELREIEGHAGRRVACH
                     WAEQILTGEVQPADALVVEAVAADPTPA"
     gene            complement(3135..3701)
                     /locus_tag="PLF56_00315"
     CDS             complement(3135..3701)
                     /locus_tag="PLF56_00315"
                     /inference="COORDINATES: protein motif:HMM:NF014520.2"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="WhiB family transcriptional regulator"
                     /protein_id="HOQ52035.1"
                     /translation="MTHRIRAERAACIDQPAVFQHELLEDPGTARRDAGSQRTLTLLT
                     GRAAAICADCPLFSSCLYQAVVDHDVAGFAAGTTAAQRTQIRVALGIKVEAEDFDAFA
                     GVVRGARQLNGDEIVRLRQANPHESLETLALRLGCSLSTVKRHLRRAREASGEVSLAV
                     VKPSLQRVLAAFHAVVGSLGRSRVQRVA"
     gene            3875..5212
                     /locus_tag="PLF56_00320"
     CDS             3875..5212
                     /locus_tag="PLF56_00320"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_014846140.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="DUF4921 family protein"
                     /protein_id="HOQ52036.1"
                     /translation="MQAFTKEPDWLTTMADGTVKQLNPFSGTEVWTVAGRGNRPLGVT
                     LPDPVPLDLTQHGRYCAFCEQRYLDTPPEKSRMVRHPDDRRETLYGIPPEDLFATVAE
                     FRRVPNLFEILSYAYWHANYRYTLPPRVEARKTAYLTSPTGRDHVLRVARAKLLAGGR
                     SPEQVDAMSVDERLASASGFFGGGHDVIISQRHFVDGATDNTQLAASGTMTTDEHAHF
                     LAFTVESIQALFNLNRYARYVVVFQNWLRPAGASFDHLHKQLVAVDERGVQAERAVAA
                     LRSNPNVFNENAVNYAGYKNLALAENEHAIAFAGFGHRFPSLEIYSKSEQCDPWEQSP
                     VELRGMSDLLHAMHAATGVEVPSNEEWHYRPIDVDVAMPWRIVLKWRISTLAGFEGAT
                     KIYLNTLDPWAIRDRVVPKLRALRDDGLIAPDLRIGDEAECRPNSLRYHSVGR"
     gene            5294..5893
                     /locus_tag="PLF56_00325"
     CDS             5294..5893
                     /locus_tag="PLF56_00325"
                     /inference="COORDINATES: protein motif:HMM:NF019656.2"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="DUF1707 domain-containing protein"
                     /protein_id="HOQ52037.1"
                     /translation="MSTPQWQNLPVPPNSDRPAGAAGRSQAGGEVWAAFSADPREAQY
                     QHLRASDADRGLAASLLADAHADGRLDQDEYEQRLNQATSAKSLGELVPVLDDLTPQR
                     ASADQGEEACMPGMLTAKNARASIAGLPRWWLGLAVMFNLIWLLSVLGTGGWIYYWPM
                     WPMLGTAIPVILGWINGGQGRPDRQQAQLPPATHGDDLR"
     gene            5958..6884
                     /locus_tag="PLF56_00330"
     CDS             5958..6884
                     /locus_tag="PLF56_00330"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_014847715.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_function: GO:0005524 - ATP binding [Evidence IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="ATP-binding cassette domain-containing protein"
                     /protein_id="HOQ52038.1"
                     /translation="MAVSVIQARSLVKSYGDLNAVDGIDFDVAAGESFGLLGPNGAGK
                     STTMRMIGGTSLRTSGTLSVLGMDPETNGPDVRANLGVIPQQDNLDTELRVRDNLITY
                     GRYFGLPRSYLQRRADDLLEFAQLTDKAGEKVDNLSGGMKRRLTIARGLINEPRILLL
                     DEPTTGLDPQARHILWDRLFRLKEEGVTLVVTTHFMDEAEQLCDRLVVMDHGRIVAEG
                     SPLQLIRQYSTREVLEVRFGSERNAVVVEQLADVAERLEVLPDRILLYTDDGEAALER
                     VTSRGLQPLTSLVRRASLEDVFLTLTGRSLID"
     gene            6877..7755
                     /locus_tag="PLF56_00335"
     CDS             6877..7755
                     /locus_tag="PLF56_00335"
                     /inference="COORDINATES: protein motif:HMM:NF013245.2"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_component: GO:0016020 - membrane [Evidence IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="ABC transporter permease"
                     /protein_id="HOQ52039.1"
                     /translation="MTDRAASTTSVATDSNRLAYTGGAVPAAVQARIARRWGWWLYAQ
                     YRLTSMRAYLQTIVIRSIGLPLLYLASMGLGLGALVDAGAGGVDGVSYLVFVAPGLLV
                     SSIVMEASGEFTYPVMSGFKWQKHFFAASASPLRPGQISVGEVVAVGLRFMAESVVFW
                     LALVLFGAVPSGMSWLVIPIATLAALAFGAPLLAFSATQTDEGAQFAFIQRFVVMPMF
                     LFAGTFFPLAAMPWYLQWVGWISPMWHGTQLARIVCFGMPNPAWLTVLHVVVLLVAAV
                     VGTWAAARIFTRRLTR"
     gene            7752..8540
                     /locus_tag="PLF56_00340"
     CDS             7752..8540
                     /locus_tag="PLF56_00340"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_015069995.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_function: GO:0042626 - ATPase-coupled transmembrane
                     transporter activity [Evidence IEA];
                     GO_function: GO:0140359 - ABC-type transporter activity
                     [Evidence IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="ABC transporter permease"
                     /protein_id="HOQ52040.1"
                     /translation="MTALSALYAGNVRAVLERGFKVIGTQNWAILVSGFFEPVFYLLA
                     MGFGLGGLVGQVTGPNGTPMTYVAFIAPALLATSAMNGAIYDSTWNVFFKMRFGRLYE
                     AMLSTSLGPLDVAAGEIAMALFRGLLYACGFLGVMAMLGIATSWWALAMVPVALLIAL
                     GFAAVGMAVTSWFTSFQQMELINIVLLPMFMFSSTLFPIDVYPPAVQWFIQALPLWHG
                     VELMRQLSIGAFGPMTAVHLAYFGVMSVAGVAFCTLRLRALFLR"
     gene            complement(8557..8901)
                     /locus_tag="PLF56_00345"
     CDS             complement(8557..8901)
                     /locus_tag="PLF56_00345"
                     /inference="COORDINATES: protein motif:HMM:NF040323.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="glyoxalase superfamily protein"
                     /protein_id="HOQ52041.1"
                     /translation="MTQVVPILRVVDVDAALEWWQQLGFRHDFTHRYGAGTTHRFMGI
                     RRDGADVFLSENQADAQGPALVFVWVPDVDAVAHRFGAEVEQHPWARDFEVTDPDGNR
                     VRVGTKEVGQSA"
     gene            9001..10866
                     /locus_tag="PLF56_00350"
     CDS             9001..10866
                     /locus_tag="PLF56_00350"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_013865942.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_function: GO:0005524 - ATP binding [Evidence IEA];
                     GO_function: GO:0042626 - ATPase-coupled transmembrane
                     transporter activity [Evidence IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="ABC transporter ATP-binding protein"
                     /protein_id="HOQ52042.1"
                     /translation="MHDFPPRIDAYTRAETARPDTRSPARFLLWTLRVQWDVLAALSI
                     AGLAWFLPSALSPYLLGRTIDAGILVGDWAATAWWASLLAAVILVGAASGIVMHTYAV
                     RGWLIALYGTQKLVTRKSVQLGHVLNRRLPTGEVLSISSSDSDIFGATFEVVARALGA
                     FGAFLLVAGLMLSTSGPLGLVVLLVAPLMVAAAVPVLRPLNRAQTAERTQNGELTSMA
                     ADIVAGLRILRGIGGELTFGDNYAVQSQKVRRLGVTAGTWQAVVDTLSVLLSGLLLVV
                     LVWLGSHEMMAGRLTLGQLISFVGYAIFMVWPLQTMFEVAQKWVRGLVSARKTIALLN
                     AELPWTEGDLPFPARPRLRDEKSGLVVEPGEFLVVVSADPDESAALADRLGRYLPTAG
                     GAPTDEPDEDELKGRAARQARAEKERRRAEIARRDAETAGTAWGVSADGLDYEHYRMD
                     SLRERVLVSDAGSQLFAGTFQQAVDPLGVHTREQAERALRTAAAEDVFDGLPQGWASR
                     VDEKGRGLSGGQRQRVVLARALLADPEVLVLVEPTSAVDAHTEEAIAERLAGHRRGRT
                     TVVMTGSPLLVHQADRVAFVAQGRQLATGTHAYLVRNHPGYRSVINRGMEDDDEH"
     gene            10856..12796
                     /locus_tag="PLF56_00355"
     CDS             10856..12796
                     /locus_tag="PLF56_00355"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_013865941.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_function: GO:0005524 - ATP binding [Evidence IEA];
                     GO_function: GO:0042626 - ATPase-coupled transmembrane
                     transporter activity [Evidence IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="ABC transporter ATP-binding protein"
                     /protein_id="HOQ52043.1"
                     /translation="MSTDTTLGSDQLALSAASWSDHPNRFELPPELSDPAPGMPALAV
                     RFRSRQARAEAFFAASRNPEQGLPVAHNAKAFAFIGSLLSTRRALLTAVIAFNVLAAT
                     AGLIVPRMLGDLVDEVAAGNTPLAAFDAIILGVAGVVVVQALLTFAARRASAVFGYDL
                     LAAAREAIVRTVLRLPLGRIESASSGDLLTRITSDVSKMSESVRWAFPQLVMSVVVTA
                     LTIVAMVLNSWFLALPLVVSITVLVLVTRVYLRRAMPGYITESGTYSQINSTMTETVE
                     GARTVEALGLHGRRIQIGEDDIAVSNQAERYTMTLRNLLFIFLDASYQLPLVAVVVLG
                     TWGYANDWVTIGQITAAAIYVQQLVEPLDRMIQVADRLQIGLAATTRLLGIAEVPQDR
                     VPGAARPVDEHLVGSELRFAYREGRDVLHGIDLDLAPGERLAVVGPSGSGKSTLGRLL
                     AGINGPRTGTVRVGEVELMTLPLDVLRTEVALVTQEHHVFVGTVRDNIVLARDHQLSP
                     EQGDAAVLDALRAVDALDWVQKLPNGLDTMLGHGRTQLTPAQAQQVALARLVVADPHT
                     LVLDEATSLIDPRTARHLEGSMAALLEGRTVIAIAHRLHTAHDADRIAVVIDGRIVEL
                     GSHAELLEQGGEYARLWHTWRS"
     gene            complement(12894..13268)
                     /locus_tag="PLF56_00360"
     CDS             complement(12894..13268)
                     /locus_tag="PLF56_00360"
                     /inference="COORDINATES: protein motif:HMM:NF037477.2"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="contact-dependent growth inhibition system
                     immunity protein"
                     /protein_id="HOQ52044.1"
                     /translation="MTGHDRRRVRPHSDVTLQQLDGEDWGDPPPDATYLIRTCHRLRR
                     QPLELFDAEDLRIMIGQRLSLPVLIPLALDVLERDPAAGGDMYEGALLDAVQAAAETR
                     ADDPAWAERLRAIIERVSIEPV"
     gene            complement(13265..14236)
                     /locus_tag="PLF56_00365"
     CDS             complement(13265..14236)
                     /locus_tag="PLF56_00365"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_014847868.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="NAD(P)H-quinone oxidoreductase"
                     /protein_id="HOQ52045.1"
                     /translation="MRAIVSNGFGGPDVLELTDVPDPVVGPGEVLIEVVASGVNRADL
                     LQRQGHYPPPDGISDIIGLEVSGRIAALGEGVDGWQLGEACVALLAGGGYAELVAVPV
                     GQVVRPPDGIDLVTAAGLIEVAATVASNLTRVALTPGEIFLVHGGTGGIGSFAIQYAV
                     ALGARAFATAGTPDKLALCRDLGAEAAFDYHDDWLGALKGATAGHGADVILDVIGAKY
                     LDLNVDGLADDGRLVVIGMQGGRKGTLDLGKLLAKRGLVTATSLRGRPLEQKAAICAE
                     VARTVWPLFSSGTIRPAQETRFPIASVRAAHEHLASGDAVGKLVLTW"
     gene            14301..14385
                     /locus_tag="PLF56_00370"
     tRNA            14301..14385
                     /locus_tag="PLF56_00370"
                     /product="tRNA-Ser"
                     /inference="COORDINATES: profile:tRNAscan-SE:2.0.12"
                     /note="Derived by automated computational analysis using
                     gene prediction method: tRNAscan-SE."
                     /anticodon=(pos:14335..14337,aa:Ser,seq:tga)
     gene            14387..14833
                     /locus_tag="PLF56_00375"
     CDS             14387..14833
                     /locus_tag="PLF56_00375"
                     /inference="COORDINATES: ab initio
                     prediction:GeneMarkS-2+"
                     /note="Derived by automated computational analysis using
                     gene prediction method: GeneMarkS-2+."
                     /codon_start=1
                     /transl_table=11
                     /product="hypothetical protein"
                     /protein_id="HOQ52046.1"
                     /translation="MSSRLHVLTPPLPLRAFGLAALLAVVGAAVIVTASAARLSPVLS
                     VIGWIALAGAVALCVVAWTTWRRMRVRVELTDEGYTIDGPDAHEQGDWSDVTKLTQSP
                     TTLVIHHGDEHRVRLVSQRGVTAELIHLTGDLVRRLDASRGYTSQL"
     gene            complement(14867..>15452)
                     /locus_tag="PLF56_00380"
     CDS             complement(14867..>15452)
                     /locus_tag="PLF56_00380"
                     /inference="COORDINATES: protein motif:HMM:NF015755.2"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=2
                     /transl_table=11
                     /product="LCP family protein"
                     /protein_id="HOQ52047.1"
                     /translation="NYRIPRGGKTSENIPPKSWIEPGPNQHLDGRDALWYARGRYHVT
                     GSDYSRMERQRCVINAVVQQAKPEVVLANFEKIASAGAKTIQTDIPRSKLPAMIDLAL
                     KVKNTSLHSVVFQPGVAGWVSANPPWPAVQRRVQQALKEADEAESARTASPTADPSVS
                     ASASASPKPTKSSTTSSGKTDNLKDACAYHPVND"
BASE COUNT         2173 a         5358 c         5374 g         2547 t
ORIGIN      
        1 cgatgaaccc cgacgaaccg cgggtcgacg acccggtggt tcccgacatc accgtcgcca
       61 cctccgtcgg cctgatcgag cggccgccgc tgctggccga ggcgatgggc gacgacgaga
      121 tcgacgaaga gggcaccagc ctgtggaaag gtgcctttca gcgcctgatc cgcaacccct
      181 ctgcgattct cggcgccgtg atcgtgaccc tcttcgtgct ggtggcgatc ctcgcccccg
      241 ttctggcccc gtacgagccg gccggctcgc agtggatcaa ccagatcacc ccgtccacgc
      301 tgccgccctt ctcgcccgag cacccgttgg gcttggaccc cttcggttcc gacctgctca
      361 cccaactgat cttcggtgcc cgccaatcgc tgatcatcgg cgtcgtctcc accctgctgg
      421 gcctgatcgg cggtgcgatc ctgggtggcc tggcgggggc cttcggtggc tgggtggacg
      481 ttgccgtcat gcgcctggtc gacattctgc tgtcgattcc gtcgctgctg ctcgccgttt
      541 cggtggccgc ggtgatgggc cgtcagccgc tggccatcat ggtcgcgatc gcagccgccc
      601 aggtgccgat cttcgcccgg ctgctgcgtg gctcgatgct ggccgagtcg cgcaaggact
      661 acgtgctcgc ctcgtggtcg ctcggcctgc gccggcgcac catcaccatg ggtcatgtgc
      721 tgcccaactc gctggggccg gtgctcgtcc aggccacctt gtcactggcc acggcgatca
      781 tcgaggtcgc tgccctgtcg tacctcggcc tcggcgaccc cgatccggcc gttgccgaat
      841 ggggacgcat gttggtcaag gcacaggacc ggttgcagtc cgcgccccac ctgaccctgt
      901 tccccggcat ctgcatcgct gtcacggccc tggggttcac cctgctcggc gaggccctgc
      961 gcgaggccct cgacccgaag tcgaggcggt gaccatgcgc gaaccgctgc tcgagatcga
     1021 caacctgagc gtcacgttcc ggcgccgccg ctcggtcacc cgcgccgtgg acggggtgag
     1081 ctactcggtc gcgcccggcg agatcgtcgg cgtggtgggc gagtcggggt ctggcaagtc
     1141 cgtcacggtg ctcgcgctga tggggctgtt gcccgatcgc ggcgtcgagg tgaccggtca
     1201 ggtccgctat gccggacgtg acctgctcag cctgggcgat tcggcgatgg cgaagctgcg
     1261 cggccgcgag atcgcgatgg tctttcagga cccgatcagt tcgctcaacc ccgtggtgcc
     1321 gatcggggtg caggtcgccg aggtgctgct cacccacacc cgcaccaccc gtagggcggc
     1381 catggccgaa tcggtcgacc tgctgcgccg gtgcggtatt cccgatcccg agcgccggct
     1441 gaaggagtac ccgcatcagc tgtccggcgg catgcggcag cgggcgctga tcgccatggc
     1501 gctggcctgc aagcccaagc tgctgatctg cgacgagccg accaccgccc tcgacgtcac
     1561 gattcaggcg caggtgctcg agttgctcaa agagctggtc accgactcgg gcacggcgat
     1621 gatcatgatc acccacgacc tgggggtggt ggccgggctg tgcgaccagg tcaacgtgat
     1681 gtactccgga cgcatcgtcg aggccgcgcc ccgcaccgaa ctgttcgcac acccccggca
     1741 ccgctacacc cacggcctgc tcgcctcgat cccgcggctc gacctgccgc gcggcgaacc
     1801 gctgcacccg attccgggca ccccgcgcga caccgtggcg tgggccaagg cctgcgcgtt
     1861 cgcgccccgc tgccggttcg ccaccgacgc gtgcgtgacg cccgacatcg ccctggcgcc
     1921 cttgtccgat tcgccgcccg atcacccggt gcgctgcgtc caccccgcgg aggcctgaga
     1981 tggttgagcc cgtcgacacc gaacccggcc gcgacgacct gctggtcgtc gagaacctgc
     2041 aggtgcactt cccgatccgc aagggcctgg tgttcgaccg caccgtcggc cacgtcaagg
     2101 cggtggacgg tgtgtcgctg cgggtgcccc gcggcagcac ctacggcctg gtcggcgagt
     2161 cgggctgtgg caagtccacg ttcggacgtg cggtgctgcg cctggaggaa ccgaccggtg
     2221 gcactgtcac gctggacggt gagagcgtca ccggcctcaa gggggagaac cttcggcagg
     2281 cccggcggcg gatgcagatg gtgttccagg atccgctggc ctcgctcgac ccgcgccaat
     2341 ccgtccagag cctgatgatc gagccgctgc gggtgcacca gcccgccccg cacgagcaac
     2401 tggtggcccg cgccgcagag atgctcgaac tggtcggcat gccccgctcg gcgctggcca
     2461 agtaccccca cgagttctcc ggcggccagc ggcagcgcat cggcatcgcg cgggcgatga
     2521 tcctcaaccc cgaactggtg atcgccgacg aaccggtctc ggccctggac gtatcggtgc
     2581 aggcccaggt gatcaacctg ctggagaagc tgcagcacga gttcggcctg acctacatcg
     2641 tgatcgccca cgacctggcg gtggtgcggc acatctcgga cgtggtcggc gtgatgtacc
     2701 tgggccggct ggtcgaagag gcgccgtcgg atgcgttgta cgcccaggcg ctgcacccgt
     2761 acacgatcgc gttgatgtcg gcgatcccga tccccgaccc cgaggtcgag gaccggcgcg
     2821 aacgcatcct gctgcgcggc gacctgccca gcccggccaa cccgccgtcc ggctgccgct
     2881 ttaacacccg gtgcccctac cggcaaccca cccgctgtga caccgacgaa cccgagttgc
     2941 gcgagatcga ggggcatgcc ggacggcggg tcgcctgcca ctgggccgag cagatcctga
     3001 ccggcgaggt gcagccggcg gacgccctgg tggtcgaggc cgtggccgcc gacccaaccc
     3061 ccgcctaact ttccagcgcc tgccttactc cgcggagtaa ggcaggcgct ggagagttag
     3121 gagggcgctc ggcgtcaggc gacccgctgc acccgagaac ggccgaggct gccgaccacg
     3181 gcgtggaagg cggccagcac ccgctgcagg gacggcttca ccaccgccag gctcacctcg
     3241 ccggacgcct cgcgggcccg ccgcaggtgc cgcttcaccg tcgagagcga gcagccgagc
     3301 cgcagcgcga gcgtctcgag gctttcgtgc gggttcgcct ggcgcagccg gacgatttcg
     3361 tcaccgttca gctgccgtgc cccccggacg acgcccgcga acgcgtcgaa gtcctcggcc
     3421 tcgaccttga tgcccagcgc cacccggatc tgcgtgcgct gggcggcggt ggtgccggca
     3481 gcaaaccctg ccacgtcgtg atcgaccacg gcctggtaca ggcacgagct gaacagcggg
     3541 cagtcggcgc agatcgccgc ggcgcgtccg gtcagcaagg tcagcgtgcg ctggctgccg
     3601 gcgtcgcgcc gggccgttcc cggatcctcc aacaactcgt gctgaaagac ggcgggctgg
     3661 tcgatgcagg cggcgcgctc ggcgcgaatg cggtgcgtca tgttcgttct cactttcgtg
     3721 actgggcctc gtcggggttg gacgcgagag gtcacgaatc ggttgcaact ccccccggaa
     3781 aaaccctgaa cccttccgcg cgcgcggtcg gctccgggct tcccttttga acgctaccct
     3841 ggccctgact acgcattcca ggggagcact acccatgcag gccttcacca aagagccgga
     3901 ttggctcacc acgatggcgg acggcaccgt caagcaactc aacccgttct cgggcaccga
     3961 ggtgtggacg gtggccggcc gcggcaatcg tccgctcggg gtgaccctgc ccgatccggt
     4021 gccgctcgac ctcacccagc acggccggta ctgcgccttc tgcgagcagc gctacctcga
     4081 caccccgccc gagaagtcgc gcatggtgcg ccaccccgac gatcgccgcg agaccctgta
     4141 cggcattcct cccgaggacc tgttcgccac cgtcgccgag tttcgccggg tgccgaacct
     4201 gttcgagatc ctcagctacg cctactggca cgccaactac cgctacacgc tgccgccgcg
     4261 cgtggaggct cgcaagaccg cctacctcac ctcgcccacc ggtcgcgacc acgtgctgcg
     4321 ggtcgcccgc gccaagctgc tggccggcgg acgttccccc gagcaggtgg acgcgatgag
     4381 cgtggacgag cgcctggcgt ccgcctcggg gttcttcggt ggcggccacg acgtgatcat
     4441 cagccagcgc catttcgtgg acggtgcgac cgacaacacg caactggcgg cgtccggcac
     4501 catgacgacc gacgagcacg cgcactttct ggccttcacc gtcgagtcga tccaggcgct
     4561 cttcaacctg aaccgctacg cccgctacgt ggtggtgttc cagaactggc tgcgcccggc
     4621 gggtgcgtcc ttcgaccacc tgcacaagca actcgtcgcc gtggacgaac gcggggtgca
     4681 ggccgagcgg gccgtggccg cgctgcgcag caacccgaac gtgttcaacg agaacgccgt
     4741 caactacgcc ggctacaaga acctggcgct ggccgagaac gagcacgcga tcgccttcgc
     4801 cggattcggc caccggtttc cctcgctcga gatctactcc aagagcgagc agtgcgatcc
     4861 gtgggagcag tccccggtcg agttgcgggg catgtcggac ctgctgcacg ccatgcatgc
     4921 cgccaccggg gtcgaagtgc cgtccaacga ggagtggcac taccgcccga tcgacgtcga
     4981 cgtcgcgatg ccctggcgca tcgtgctgaa gtggcgcatc tcgaccctgg ccggtttcga
     5041 aggcgccacc aagatctacc tcaacaccct cgacccctgg gcgatccgcg atcgcgtggt
     5101 accgaagctg cgcgcgctgc gcgacgacgg gctgatcgcg cccgatctgc gcatcggcga
     5161 cgaggccgag tgccgtccca actcgctgcg ctaccacagc gtcggacgct gagcgcactg
     5221 ggggtaacct caggggccac cggccgccgg tccggggcac aatggacgga ctcgcgagcg
     5281 aaggtgaacc ccgatgtcga ctccgcaatg gcagaacctg cccgtaccgc caaactccga
     5341 caggccggcc ggtgccgctg gtcggtccca ggccggtggt gaggtgtggg cggccttctc
     5401 ggccgacccg cgcgaggccc agtaccaaca cctgcgcgcc tcggacgccg accgcggcct
     5461 cgccgcctcg ctgctggccg acgcccacgc cgacggacgg ctcgatcagg acgaatacga
     5521 gcagcggctg aaccaggcga ccagcgccaa gagcctgggt gagctggtgc cggtactgga
     5581 cgacctgacc ccgcagcggg cgtccgccga ccagggcgag gaggcctgca tgccgggcat
     5641 gctcaccgcc aagaacgccc gggcctcgat cgcgggactg ccgcgctggt ggctgggcct
     5701 ggcggtgatg ttcaacctga tctggctgtt gagcgtgctg ggtaccggcg gctggatcta
     5761 ctactggccg atgtggccca tgttgggcac cgcgatcccg gtgatcctgg gctggatcaa
     5821 cggtggtcag ggtcgtcctg accggcagca ggcccagttg ccacccgcga cccacggcga
     5881 cgacctgcgc tgattgctgc attgcggggg tgccgggcat ccgctttcag tttctgtcgg
     5941 acgcggttgg taggttgctg gccgtgtccg tgatccaggc ccgctcgctg gtcaagagct
     6001 atggcgacct caacgccgtc gacggcatcg acttcgacgt cgccgccggt gagtcgttcg
     6061 gcctgctcgg gcccaacggt gcgggcaagt ccaccaccat gcgcatgatc ggcggcacgt
     6121 cgctgcgcac gtccggcacc ctgtcggtgc tgggcatgga tcccgaaacc aacggccccg
     6181 acgtgcgggc caacctgggc gtcattccgc agcaggacaa tctcgacacc gagttgcggg
     6241 tgcgcgacaa cctgatcacc tacggccgct acttcgggct gccgcgcagc tacctgcagc
     6301 gccgtgctga cgacctgctc gagttcgcgc agctcaccga caaggcgggc gagaaggtcg
     6361 acaacctctc cggcggcatg aagcgccggc tcaccatcgc ccgcgggctg atcaacgagc
     6421 ccagaatcct gctgctggac gaacccacca ccggcctcga cccgcaggcc cggcacatct
     6481 tgtgggaccg gctgttccgg ctcaaagaag agggcgtcac gctggtggtc accacccact
     6541 tcatggacga ggccgagcag ctgtgcgacc ggctggtggt gatggatcac ggacgcatcg
     6601 tggctgaggg ttcgccgctg cagctgatcc ggcagtactc cacccgcgag gtgctcgagg
     6661 tgcgcttcgg ctccgaacgc aacgccgtgg tggtcgaaca gttggccgac gtggccgagc
     6721 ggctcgaggt gctgcccgac cgcattctgc tctacaccga cgacggcgag gcggcgctcg
     6781 agcgcgtcac ctcgcgcggg ctgcagccgc tcacctcgct ggtgcgacgc gcgtccctcg
     6841 aagacgtgtt tctgacgctc accggaaggt cgctcattga ctgaccgggc agcgtcgacg
     6901 acctcggtcg ccaccgactc caaccgcctc gcctacaccg gcggggcggt gccggccgcc
     6961 gtgcaggcac gcatcgcccg gcgctggggg tggtggctgt acgcgcagta ccggttgacg
     7021 tcgatgcgcg cctacctgca gaccatcgtg atccgctcga tcggcctgcc gctgctgtac
     7081 ctggcttcga tggggctggg gctgggcgcc ctggtcgacg cgggagcggg cggtgtcgac
     7141 ggggtcagct acctggtgtt cgtggcgccc gggttgctgg tgtcgtccat cgtgatggag
     7201 gccagtggcg agttcaccta cccggtgatg agcggcttca agtggcagaa gcacttcttc
     7261 gccgcctcgg cgtcgccgct tcgtccgggg cagatctcgg tcggcgaggt ggttgccgtg
     7321 gggctgcgat tcatggccga gtcggtggtg ttctggctgg cgttggtgct gttcggcgcg
     7381 gtgccgtccg gcatgtcgtg gctggtgatc ccgatcgcga ccctggccgc cctggcgttc
     7441 ggggcgccgc tgctggcctt ctcggcgacg cagaccgacg agggcgcgca gttcgcgttc
     7501 attcagcggt tcgtggtgat gccgatgttc ctgttcgcgg gcaccttctt tccgctcgcc
     7561 gcgatgccct ggtacctgca gtgggtcggc tggatctcgc cgatgtggca cggcacccag
     7621 ctggcccgca tcgtctgctt cggtatgccc aacccggcct ggctgacggt gctgcacgtg
     7681 gtggtgctgc tggtcgcggc ggtggtcggc acctgggccg cggcccgcat cttcacccga
     7741 aggctcaccc gatgaccgcg ctcagcgccc tgtatgcggg caacgtccgg gcggtgctcg
     7801 agcgcggctt caaggtgatc ggcacccaga actgggcgat tctggtctcg ggcttcttcg
     7861 agcccgtctt ctacctgctg gcgatgggtt tcggactggg cgggctggtc ggtcaggtga
     7921 ccgggccgaa cggcacgccg atgacctacg tggcgttcat cgcgccggcg ctgctggcca
     7981 cctcggccat gaacggcgcc atctacgaca gcacctggaa cgtgttcttc aagatgcggt
     8041 tcggccgcct ctacgaggcc atgctcagca cctcgctggg tccgctggac gtggccgccg
     8101 gcgaaatcgc gatggcgctg ttccgaggcc tgctgtatgc ctgcggattt ctgggggtga
     8161 tggcgatgct cggcatcgcc acgtcgtggt gggcgctcgc gatggtgccg gtcgccctgc
     8221 tgatcgcgct ggggttcgcc gccgtcggca tggcggtgac cagctggttc acgtcgttcc
     8281 agcagatgga gctgatcaac atcgtgctgt tgccgatgtt catgttcagc tcgacgctgt
     8341 ttccgatcga cgtgtacccg ccggccgtgc agtggttcat ccaggcactt ccgctgtggc
     8401 acggcgtcga gttgatgcgc cagctctcga tcggggcgtt cggcccgatg acggccgtgc
     8461 acctggccta cttcggcgtg atgtccgtgg cgggcgtggc cttctgcacc ctgcggctgc
     8521 gggcgttgtt cctgcgctga tcgggccgcg cggggttcag gccgactggc cgacctcttt
     8581 ggtgcccacc cggactcgat tgccgtcggg atcggtcacc tcgaagtcgc gggcccacgg
     8641 gtgctgctcg acctcggcgc cgaaccggtg ggcgacggcg tccacgtcgg gcacccacac
     8701 gaacaccagc gccgggccct gggcgtcggc ctggttctcg ctgagaaaga cgtcggcgcc
     8761 gtcacgtcgg atgcccatga accggtgtgt ggtgccggcg ccgtagcgat gggtgaagtc
     8821 atggcgaaaa cccagctgct gccaccattc cagggccgcg tcgacgtcga cgacgcgaag
     8881 gatcggaacc acctgagtca ttcactgcct ccttgccgaa tggctttggt ccatgatgcc
     8941 tgccggacgt cgatcgtgcg ggtcgacaag ccgcgggccg aagcgtaacg ttgcccggct
     9001 atgcatgatt tcccaccgcg gatcgacgcc tacacgcgcg ccgaaaccgc caggccggac
     9061 acgcgcagcc cagcacggtt tctgctgtgg acgctgcgcg tgcaatggga cgtgctcgca
     9121 gccctgagca tcgcagggct ggcctggttt ctgcccagtg cgttgagccc ctacctgctc
     9181 ggacgcacca tcgacgccgg gatcctggtc ggcgactggg ccgccaccgc gtggtgggcc
     9241 tcgctgctgg cggcggtgat cctggtcggc gcggccagcg gaatcgtgat gcacacctac
     9301 gcggtgcggg gctggctgat cgcgctgtac ggcacgcaga agctggtcac ccgcaagtcg
     9361 gtgcagttgg ggcacgtgtt gaaccgccgg ctgccgaccg gtgaggtact gagcatcagt
     9421 tcgtccgact ccgacatctt cggggccacg ttcgaggtgg tcgcccgcgc gttgggcgcc
     9481 ttcggggcgt ttctgctggt cgccgggctg atgctgagca cgtccggacc gctggggctg
     9541 gtggtgctgc tggtcgcgcc gctgatggtg gccgcggcgg tgccggtgct gcgtccgctg
     9601 aaccgggccc agaccgccga acgcacccag aacggcgagc tcacctcgat ggccgccgac
     9661 atcgtggccg gcctgcgcat tctgcgcggc atcggcggcg agttgacctt cggcgacaac
     9721 tacgccgtgc agtcgcagaa ggtgcgccgg ctcggcgtca ccgccggcac ctggcaggcc
     9781 gttgtcgaca cactgtcggt gctgctgtcg gggttgttgc tggtcgtgct ggtctggctc
     9841 gggtcgcacg agatgatggc cgggcggctc accctggggc agttgatcag cttcgtcggc
     9901 tacgcgatct tcatggtgtg gccgctgcag accatgttcg aggtggcgca gaagtgggtg
     9961 cgcggcctgg tgtcggcacg caagacgatc gccctgctga acgccgagtt gccctggacc
    10021 gagggcgacc tgccgtttcc cgcccggcca cggttgcggg acgagaagtc ggggttggtg
    10081 gtcgagccgg gcgagtttct ggtcgtcgtc agcgccgacc cggacgagtc ggccgccctg
    10141 gccgaccggc tgggtcgcta cctgcccacg gccgggggcg cgcccaccga cgagccggac
    10201 gaggacgaac tgaagggccg ggccgcgcgg caggcgcgcg ccgagaagga gcggcgccgg
    10261 gcggagatcg cgcggcgcga tgccgagacc gccggcacag cctggggcgt gagcgcggac
    10321 gggctcgatt acgagcacta ccgcatggac tccctgcgcg agcgggtgct ggtctcggac
    10381 gccggcagcc agttgttcgc cggcaccttt cagcaggccg tcgatccact gggggtgcac
    10441 acccgcgagc aggcggagcg cgcgttgcgc accgcggcgg ccgaggacgt cttcgacggc
    10501 ttgccgcagg gctgggcaag tcgggtcgac gagaagggtc gcgggctctc gggcggtcag
    10561 cggcagcggg tcgtgctggc tcgggcgctg ttggccgacc ccgaggtgct ggtgctggtc
    10621 gagcccacgt ccgccgtgga cgcccacacc gaagaggcga tcgccgagcg gctggccggc
    10681 catcggcgtg gacgcaccac cgtggtgatg accggttcgc cgctgctggt gcatcaggcc
    10741 gaccgggtgg cgttcgtggc gcagggccgt cagctggcca ccggcaccca tgcgtacctg
    10801 gtgcgcaacc atccgggcta ccgatccgtg atcaatcgtg gaatggagga cgacgatgag
    10861 cactgacacg acgctgggct ccgatcagct tgccttgtcg gccgccagtt ggtccgatca
    10921 tccgaaccgt ttcgagttgc cgccggagct gagcgatccc gcaccgggaa tgccggctct
    10981 cgcggtccgg ttccgtagcc ggcaggccag ggcggaggcg ttcttcgcgg cgtccagaaa
    11041 ccccgagcag gggctgccgg ttgcgcacaa cgcgaaggcc ttcgcgttca tcggctcgct
    11101 gctgtcgacg cggcgcgccc tgctgaccgc cgtgatcgcc ttcaacgtgc tggccgccac
    11161 ggccgggctg atcgtgccgc gcatgctggg cgatctggtg gacgaggtcg cggccggcaa
    11221 cacgccgttg gctgcgttcg atgcgatcat cctcggcgtc gccggtgtcg tggtggtgca
    11281 ggcactgctc accttcgcgg cgcgccgcgc gtccgccgtg ttcggctacg acctgctggc
    11341 cgccgcccgc gaggcgatcg tgcgcaccgt gctgcggctg ccgctggggc ggatcgaatc
    11401 ggccagctcg ggcgacctgc tgacccgcat cacctccgat gtgtcgaaga tgagcgaatc
    11461 ggtgcgttgg gcgtttccgc aactggtgat gtcggtggtg gtgaccgcgc tgaccatcgt
    11521 ggcgatggtg ctcaactcat ggttcctggc gctgccgctg gtggtgtcga tcaccgtgct
    11581 ggtcctggtg acaagggtgt acctgcggcg cgcgatgccc ggctacatca ccgagtcggg
    11641 cacctattcg cagatcaact ccaccatgac cgaaaccgtc gagggggctc gcacggtcga
    11701 ggcgctgggg ttgcacgggc ggcggatcca gatcggcgag gacgacatcg cggtgtcgaa
    11761 ccaggccgag cggtacacca tgacgctgcg caacctgctg ttcatctttc tggacgccag
    11821 ctaccagctg ccgctggtcg cggtggtggt gctgggcacc tggggctacg ccaacgactg
    11881 ggtgacgatc ggccagatca ccgccgccgc aatctatgtg cagcaactgg tggagccgct
    11941 ggaccggatg atccaggtgg ccgaccggct gcagatcggc ctcgcggcca ccacccgact
    12001 gctcggcatc gccgaggtgc cgcaggatcg ggtgccgggc gcggctcgtc cggtcgatga
    12061 gcatctggtg ggcagcgagt tgcgcttcgc ctaccgcgag ggccgcgacg tgctgcacgg
    12121 catcgatctc gacctcgcgc cgggggagcg gctggccgtg gtgggaccgt ccgggtcggg
    12181 caagtcgacg ctcggacggc tgctggccgg catcaacggg ccgcgcaccg gcaccgtccg
    12241 ggtcggcgag gtcgagctga tgaccctgcc gctggacgtg ctgcgcaccg aggtggcgct
    12301 ggtcacgcag gagcatcacg tgttcgtggg caccgtccgc gacaacatcg tgctggcccg
    12361 cgaccaccag ctgtcgcccg agcagggcga cgcggcggtg ctcgacgcac tacgcgcggt
    12421 ggatgccctc gattgggtgc agaagttgcc gaacgggctc gacaccatgc tcggccacgg
    12481 acgcacgcag ctcaccccgg cgcaggcgca gcaggtggcg ttggcccggc tcgtggtggc
    12541 cgatccgcac accctggtgc tcgacgaggc cacctcgttg atcgacccgc gcacggcgcg
    12601 tcacctggag gggtcgatgg cggcgctgtt ggagggacgc acggtgatcg cgatcgcgca
    12661 ccgcctgcat acggcccacg acgcggaccg gatcgcggtg gtgatcgacg gccggatcgt
    12721 tgagctgggc tcccacgccg agctgctcga acagggcggc gagtacgccc ggctgtggca
    12781 cacctggcgt tcctgacttc gcaccccgtc atcccggcgc accccgtcat cccggcggac
    12841 gccgggatct cgaccaccgg ccggcgtgat tgggcaatcg agggatgacg aggctagacg
    12901 ggctcgatcg acacccgctc gatgatcgcg cggagccgct cggcccacgc cgggtcgtcc
    12961 gcccgcgtct cggcggccgc ctgcacggcg tcgagcaggg ccccctcata catgtcgccg
    13021 ccggccgccg ggtcgcgctc gagcacgtcc agcgccagcg ggatcagcac cggcaggctc
    13081 aaccgttggc cgatcatgat ccgcagatcc tcggcgtcga acaactccaa cggctgccgg
    13141 cgcagccgat ggcaggtgcg gatcaggtag gtggcatccg gtggcggatc accccaatcc
    13201 tccccgtcga gttgctgcag ggtgacgtcg ctgtgcggcc ggacgcgccg tcgatcgtgg
    13261 ccggtcacca ggtgagcacc agcttgccga ccgcgtcccc cgaagccaga tgctcatggg
    13321 cggcacgcac gctggcgatc gggaaccggg tctcctgtgc cggacggatc gtaccgctgg
    13381 agaacagcgg ccacacggtg cgcgccacct cggcgcagat cgccgccttc tgttccagcg
    13441 gacgtccgcg caggctggtc gccgtcacca ggccgcgttt ggccagcagc ttgcccagat
    13501 cgagcgtgcc cttgcgtccg ccctgcatgc cgatcaccac cagccgtccg tcatcggcca
    13561 gcccatccac gttgaggtcc aggtacttgg cgccgatcac gtccaggatc acgtcggccc
    13621 cgtggcccgc ggtggcaccc ttgagcgcgc cgagccagtc gtcgtggtag tcgaaggccg
    13681 cctcggcgcc gaggtcgcgg cacagcgcca acttgtcggg ggtgccggcg gtcgcgaacg
    13741 cccgcgcgcc cagcgcgacc gcgtactgga tcgcgaagct gccgatgccg ccggtgccgc
    13801 cgtgcacgag aaagatctcg cccggcgtca gcgccacccg ggtgaggttg gacgccaccg
    13861 tggccgccac ctcgatcagc ccggcggcgg tgaccaggtc gatgccgtcc ggcggacgca
    13921 ccacctgacc caccggtacc gccaccagtt cggcgtagcc gccgccggcc agtagagcca
    13981 cgcaggcctc gcccagctgc cagccgtcga cgccctcacc gagcgcggcg atgcgtccgg
    14041 agacctcgag gccgatgatg tcgctgatgc cgtccggtgg cgggtagtgg ccctggcgtt
    14101 gcagcagatc cgcccggttc acccccgagg ccaccacctc gatcagcacc tcaccggggc
    14161 caacgacggg gtcgggtacg tcggtcaact caaggacgtc cgggccgccg aagccgttgg
    14221 acactatcgc gcgcatgtct ccaccctggc atgcgggacc ggcgttgacc accacggaca
    14281 accattagga tggagcgctg ggcgaggtcg catagtggac tagtgcagcc gccttgaaag
    14341 cggccgagcg gaaacgctcc gtgggttcga atcccaccct cgccgcgtgt cgtcccgtct
    14401 tcatgtgctc accccacccc tgccgctgcg cgccttcggg ctggcggccc tgctggcggt
    14461 ggtcggcgcg gcagtgatcg tgaccgcctc ggccgctcgg ctgtcgccgg tgctgagcgt
    14521 gatcggctgg atcgctctgg cgggcgcggt tgcgctgtgc gtggtggcct ggacgacctg
    14581 gcgccgcatg cgcgtgcgcg tcgagctcac cgatgagggt tacaccatcg acggtccgga
    14641 cgcccacgag cagggcgact ggtcggacgt caccaagctc acccagtcac ccaccaccct
    14701 ggtgatccac catggcgatg agcaccgggt gcggctggtc agccaacgcg gcgtgaccgc
    14761 cgagctcatt cacctgaccg gtgacctggt gcgccggctg gacgccagcc gcggctacac
    14821 ctcgcagctg tagcggatgc ccaccgggct gggcgcgggt gaggtgtcag tcgttcaccg
    14881 gatggtaggc gcaggcgtcc ttgaggttgt ccgtcttgcc ggacgaagtc gtgctcgact
    14941 tggtcggttt cggtgaggcc gatgcactgg ccgagacgga cgggtcggcc gtcgggctcg
    15001 cggtgcgggc gctctcggcc tcgtccgctt ctttgagggc ctgttgcacc cggcgctgga
    15061 cggccggcca cggcgggttg gcgctgaccc agccggcgac gccgggctgg aacacgacgc
    15121 tgtgcaggct ggtgttcttc accttcaggg ccaagtcgat catggcgggc agcttgctgc
    15181 gcggaatgtc ggtctggatc gtcttggcgc cggccgaggc gatcttctcg aagttggcca
    15241 gcaccacctc gggcttggcc tgctgcacca ccgcgttgat cacgcagcgc tgccgctcca
    15301 tgcggctgta gtcagatccg gtgacgtggt agcggccgcg ggcgtaccac agggcgtccc
    15361 gaccgtccag gtgctggttc gggccgggct cgatccacga cttcggcgga atgttctcgg
    15421 aggtcttgcc gccgcgcgga atgcggtagt tc
//
LOCUS       DAOSUG010000006        14953 bp    DNA     linear   ENV 10-JUN-2024
DEFINITION  MAG TPA_asm: Micropruina sp. isolate AMR_MDS_2195
            NODE_2423_length_14953_cov_4.501275, whole genome shotgun sequence.
ACCESSION   DAOSUG010000006 DAOSUG010000000
VERSION     DAOSUG010000006.1
DBLINK      BioProject: PRJNA925477
            BioSample: SAMN32864262
            Sequence Read Archive: SRR4236673
KEYWORDS    WGS; ENV; Metagenome Assembled Genome; MAG; Third Party Data; TPA;
            TPA:assembly.
SOURCE      Micropruina sp. (wastewater metagenome)
  ORGANISM  Micropruina sp.
            Bacteria; Bacillati; Actinomycetota; Actinomycetes;
            Propionibacteriales; Nocardioidaceae; Micropruina.
REFERENCE   1  (bases 1 to 14953)
  AUTHORS   Abdulkadir,N., Saraiva,J.P., Zhang,J., Stolte,S., Gillor,O.,
            Harms,H. and Rocha,U.
  TITLE     Genome-centric analyses of 165 metagenomes show that mobile genetic
            elements are crucial for the transmission of antimicrobial
            resistance genes to pathogens in activated sludge and wastewater
  JOURNAL   Microbiol Spectr 12 (3), e0291823 (2024)
   PUBMED   38289113
REFERENCE   2  (bases 1 to 14953)
  AUTHORS   Abdulkadir,N.
  TITLE     Direct Submission
  JOURNAL   Submitted (24-MAR-2023) Environmental Microbiology, Helmholtz
            Center for Environmental Research -UFZ, Permoserstr 15, Leipzig
            04318, Germany
COMMENT     The annotation was added by the NCBI Prokaryotic Genome Annotation
            Pipeline (PGAP). Information about PGAP can be found here:
            https://www.ncbi.nlm.nih.gov/genome/annotation_prok/
            
            ##Genome-Assembly-Data-START##
            Assembly Method        :: SPAdes v. 3.11.1
            Genome Representation  :: Full
            Expected Final Version :: Yes
            Genome Coverage        :: Not Applicable
            Sequencing Technology  :: Illumina MiSeq
            ##Genome-Assembly-Data-END##
            
            ##Genome-Annotation-Data-START##
            Annotation Provider               :: NCBI
            Annotation Date                   :: 05/04/2023 04:57:10
            Annotation Pipeline               :: NCBI Prokaryotic Genome
                                                 Annotation Pipeline (PGAP)
            Annotation Method                 :: Best-placed reference protein
                                                 set; GeneMarkS-2+
            Annotation Software revision      :: 6.5
            Features Annotated                :: Gene; CDS; rRNA; tRNA; ncRNA
            Genes (total)                     :: 2,776
            CDSs (total)                      :: 2,750
            Genes (coding)                    :: 2,714
            CDSs (with protein)               :: 2,714
            Genes (RNA)                       :: 26
            tRNAs                             :: 24
            ncRNAs                            :: 2
            Pseudo Genes (total)              :: 36
            CDSs (without protein)            :: 36
            Pseudo Genes (ambiguous residues) :: 0 of 36
            Pseudo Genes (frameshifted)       :: 2 of 36
            Pseudo Genes (incomplete)         :: 32 of 36
            Pseudo Genes (internal stop)      :: 2 of 36
            ##Genome-Annotation-Data-END##
FEATURES             Location/Qualifiers
     source          1..14953
                     /organism="Micropruina sp."
                     /mol_type="genomic DNA"
                     /submitter_seqid="NODE_2423_length_14953_cov_4.501275"
                     /isolate="AMR_MDS_2195"
                     /isolation_source="Activated sludge of municipal
                     wastewater treatment plants"
                     /db_xref="taxon:2737536"
                     /environmental_sample
                     /geo_loc_name="Canada"
                     /lat_lon="49.25 N 123.1 W"
                     /collection_date="2015-06-16"
                     /metagenome_source="wastewater metagenome"
                     /note="metagenomic"
     gene            <1..412
                     /locus_tag="PLF56_00385"
     CDS             <1..412
                     /locus_tag="PLF56_00385"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_016670157.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=2
                     /transl_table=11
                     /product="translation initiation factor IF-2"
                     /protein_id="HOQ52048.1"
                     /translation="KATQMADSAGVDIRYYSVIYAAIDDVEAALKGMLKPIFEEKVSG
                     QAQIREIFRSSKFGTIAGCMVIDGSIRRNSKARLIRDGVVVTESSIASLRREKDDVTE
                     VREGYECGMTLANYSDIKVDDVIETFEMVEKPRD"
     gene            414..842
                     /gene="rbfA"
                     /locus_tag="PLF56_00390"
     CDS             414..842
                     /gene="rbfA"
                     /locus_tag="PLF56_00390"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_015070867.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_function: GO:0019843 - rRNA binding [Evidence IEA];
                     GO_process: GO:0006396 - RNA processing [Evidence IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="30S ribosome-binding factor RbfA"
                     /protein_id="HOQ52049.1"
                     /translation="MGNPRYIKVAEQIKVIVAEMLERRIKDPRLGFVTVTDVRLTGDG
                     REGTVFYTVLGDEQAQADTAAALESARGLIRSTVGKQLGMKFTPTIAFQLDAVPETAS
                     QITEALARAKAADEAVAAQAATASYAGDADPYKKADEEQE"
     gene            1137..1724
                     /locus_tag="PLF56_00395"
     CDS             1137..1724
                     /locus_tag="PLF56_00395"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_012921153.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="GNAT family N-acetyltransferase"
                     /protein_id="HOQ52050.1"
                     /translation="MTDYRVEALDATTWPAFEALVSKHNGIFGGCWCMWFHDRSDTGP
                     GAEGNRSAKQQLVQRGATHHALVMHGDQAVGWCEYGTPAELPNIYHRKQYDASGIAPT
                     TYRITCFFVDRDHRRRGVAELALTGALDAIARAGGGSVEGFPRHDLDGRKLSASFLFN
                     GTLHMFERAGFTRVADLAKFRCIMRRNVDAAVALT"
     gene            1729..2619
                     /gene="truB"
                     /locus_tag="PLF56_00400"
     CDS             1729..2619
                     /gene="truB"
                     /locus_tag="PLF56_00400"
                     /EC_number="5.4.99.25"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_016670155.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_function: GO:0004730 - pseudouridylate synthase
                     activity [Evidence IEA];
                     GO_process: GO:0006400 - tRNA modification [Evidence IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="tRNA pseudouridine(55) synthase TruB"
                     /protein_id="HOQ52051.1"
                     /translation="MSDSGILVVDKPSGWTSHQVVGRCRRLLGTRKVGHAGTLDPMAT
                     GVLVIGVERGTRLLGHLALTTKTYEATIRLGQATNTDDADGELTAAQGASGITDEAIE
                     DALAVLRGDIVQVPSTVSAIKINGQRAYALARKGEDVRLAGRPVTVSRFDVLARRNTE
                     ADGVPVVDLDVVVDCSSGTYIRALARDLGAALGTGGHLTSLRRTRVGGFDLTGALRPD
                     ALTADPPQRPALIGLAAVARDHFAFVELGDDEARDVGYGRPLRRPVPADPTALLHGQE
                     LLALYRPAGERAVPVAVLVG"
     gene            2779..4194
                     /locus_tag="PLF56_00405"
     CDS             2779..4194
                     /locus_tag="PLF56_00405"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_012921118.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_component: GO:0016020 - membrane [Evidence IEA];
                     GO_function: GO:0022857 - transmembrane transporter
                     activity [Evidence IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="APC family permease"
                     /protein_id="HOQ52052.1"
                     /translation="MSATEQTETGQPNELKRVMGPGLLLLFIIGDILGTGIYALTGVV
                     AKEVGGVVWLPFLIAFVVATITAYSYLELVTKYPQAAGAALYAHRAFGIHFLTFIVAF
                     TVMTSGITSASTAARAFAENLQKGFGLDASAGLTTVIAIGFMLLVAAVNVRGVGESVK
                     ANVVLTCIELSGLLMVIFVAVFAMSEGRADFSRVTVFHTPEDKGVFIAVTTATSLAFF
                     AMVGFEDSVNMAEETKEPSRIFPKIMLTGLGITGVIYVLVSISAVALVPPGELSKEGA
                     TPLLLVVQTGAPDLPIGAIFPFISMFAVANSALINMLMASRLLYGMANEGVLPPAFGR
                     VLKGRRTPWVAILFTTALAVGLITFVGQVSQLGGTTALLLLGVFTLVNISVLVLRKDP
                     VEHDHFTAPTWLPIVGALSCAYLVGPWTGRSLEQYWIGAVLIGIGVVLSLVMFLVNRR
                     RHKPTRFEHPDDDLRSFGPLN"
     gene            4295..5368
                     /locus_tag="PLF56_00410"
     CDS             4295..5368
                     /locus_tag="PLF56_00410"
                     /inference="COORDINATES: protein motif:HMM:NF013648.2"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="siderophore ABC transporter substrate-binding
                     protein"
                     /protein_id="HOQ52053.1"
                     /translation="MSVSTNKSPQQHLSVHTRTRRALPGALMAVVALLTTACSAGTPA
                     ASTPAGPTVVIENAKPTLKQVEADGTTSMQENLEIARAAVTVPQKPTKVVTFDIATLD
                     TLDAIGAGDSVVGVPAIGLPDYLVKYADLPKVGTLFEPDFEAVSKLGPDLIVTAARST
                     GAYDELSRIGITIDLTAKYAGAFDPDAALVRAKQLGTIFDKNDEVTKLADAITAKQQS
                     LQAEAKKAGSALLLSVSGGEYGAFGEGSRFGYFFDTLGFTPAVPAAELPGAKGSSHGD
                     TVSNEFILKANPDWIIAFDRGAATGEPASAEQTLTNELVAKTTAGQNKQIIYLPASEL
                     YIVINGLTAIDRVLDSVHGALTT"
     gene            5373..6398
                     /locus_tag="PLF56_00415"
     CDS             5373..6398
                     /locus_tag="PLF56_00415"
                     /inference="COORDINATES: protein motif:HMM:NF013219.2"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_component: GO:0016020 - membrane [Evidence IEA];
                     GO_function: GO:0022857 - transmembrane transporter
                     activity [Evidence IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="iron chelate uptake ABC transporter family
                     permease subunit"
                     /protein_id="HOQ52054.1"
                     /translation="MLTRPTLLIIAGVVLVTALATASVLVGVAELDGFVLLASRLPRT
                     VALVLTGAAMAVTGLIMQLLTHNRFVEPTTTGAGDAASFALLMLLLTNPGLPLPGKAA
                     VATLGALVGVFGFLTLARRIPVRSSVLVPVVGIMYGGVIGAVTTFIAYRTELLQELLS
                     WSIGDFSGIVRGRYEMLYLAAAAALVAWIAADRFTVAALGDDTARGLGLDSRGVLLLG
                     VTIVAVVTGIVSVTTGLVPFLGLVVPNLVSRLIGDHMRRAVPLVALLGAALVLACDLV
                     GRLVRFPFEIPISIVMGIVGGTVFLWLLLGPASRTGGATRSGNASKAGRATGTAARRA
                     EAVGRRG"
     gene            6391..7419
                     /locus_tag="PLF56_00420"
     CDS             6391..7419
                     /locus_tag="PLF56_00420"
                     /inference="COORDINATES: protein motif:HMM:NF013219.2"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_component: GO:0016020 - membrane [Evidence IEA];
                     GO_function: GO:0022857 - transmembrane transporter
                     activity [Evidence IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="iron chelate uptake ABC transporter family
                     permease subunit"
                     /protein_id="HOQ52055.1"
                     /translation="MAEPTVTERGTRPAGGTTSHAPARRRVRFGGGARIGLLGALAAG
                     AVALYLFHDLPERWQYPMDLRTRTVAAMLLVAVAVGVSTVLFQTITANRILTPGIMGF
                     DAVFMAIQVFTVFALGPMVLLAAPPLVSWLVELVLMSGFVVALYWGLFLGRRLDLHVI
                     VLAGLVLGTVFRSVTAFLQRLLDPDTFAIVQNLAFASFTSVDRTLLLPTALLVLAALA
                     SLWPIRRSLDVLSLGEAPAIGLGVEHRRVVMHVIVVVALMVGASTALVGPVMFFGLLV
                     ANVAYSVVGHRHSRALPAACLIGLIALVGGQWFLDRVLGFATELPVVIEFLGGVVFIV
                     LVLTGRAR"
     gene            7424..8176
                     /locus_tag="PLF56_00425"
     CDS             7424..8176
                     /locus_tag="PLF56_00425"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_013160595.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_function: GO:0005524 - ATP binding [Evidence IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="ATP-binding cassette domain-containing protein"
                     /protein_id="HOQ52056.1"
                     /translation="MIRTEALTKRHGEIVAVDDVTIELGDGLTAIIGPNGAGKSTLLC
                     AIGRLIRADAGRVVVGGLDVATTRSRELAKRLAVLRQENHLPIRLSVQELVAFGRFPH
                     GGTGRSPRDRTHIEGALDLLGLDEVRDRFLDELSGGQRQRAFVAMALAQDTDYLLLDE
                     PLNNLDPRHAVELMRLLTGLAAERGLRIVVVLHDLNVAARYADDVVAMREGRVVAQGA
                     VEQVITVEQLTDLYDTPAHVVEVEGRRVVLWQ"
     gene            8275..8526
                     /locus_tag="PLF56_00430"
     CDS             8275..8526
                     /locus_tag="PLF56_00430"
                     /inference="COORDINATES: ab initio
                     prediction:GeneMarkS-2+"
                     /note="Derived by automated computational analysis using
                     gene prediction method: GeneMarkS-2+."
                     /codon_start=1
                     /transl_table=11
                     /product="hypothetical protein"
                     /protein_id="HOQ52057.1"
                     /translation="MPNAELDAKLAGNFARAIDFKAVGETVADVGGALYDDDGWIRWC
                     GSDFRIFKWRGFELHDELIKELTINLRELNAKLGEGRIG"
     gene            8523..9713
                     /locus_tag="PLF56_00435"
     CDS             8523..9713
                     /locus_tag="PLF56_00435"
                     /inference="COORDINATES: ab initio
                     prediction:GeneMarkS-2+"
                     /note="Derived by automated computational analysis using
                     gene prediction method: GeneMarkS-2+."
                     /codon_start=1
                     /transl_table=11
                     /product="hypothetical protein"
                     /protein_id="HOQ52058.1"
                     /translation="MTSTLAAPAGSLTRPAPSVIDARWIERGVGVCERVLDVVLTTRD
                     QSAVAEVGASIAKVVAESAILLRILKPWHHCVLAARLAERLAVLAVDPRTLTALALHA
                     SAAFELAVPYLVLRERHRDDRVDDLLHTAFAARAAQGRELLPHRALERLWLLGLLTGR
                     REFTRTDLEATVLAREIDLLHGTRDDHYAFTHALMYATDFGRDPDAVPADWKPRLVDQ
                     ARSALAGALDEDDYDLAGELLLTWPFLRETAGPVESTALMVLCDVADAVGTLPSLTLA
                     LVEGTDRLPAADAVVLSTYHTSFVFTALAALAPRVEWRTSAANGPSLRATWPSGGRRP
                     GWLDVPAAGLPAGPLGLEAGVRRAVREHRLADVAALLSAPGTPATPATNQALQLLERL
                     SLLP"
     gene            9940..11412
                     /locus_tag="PLF56_00440"
     CDS             9940..11412
                     /locus_tag="PLF56_00440"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_015071598.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="phosphatase PAP2 family protein"
                     /protein_id="HOQ52059.1"
                     /translation="MTPPIDGRLRRGRALAATAATLALLASTGLTNAQAATSYPSDTA
                     KPDLMPALSGYSDLWQSSGLNDLHGTVKNSTVLQWNDRVTSWINQHATAKQQFRALQN
                     SNYLASDGSGYDQSISIADGLGKKLGALYAQGRIEKKLPLVAALINSSTGATGAYVST
                     GAAKAAFSYPRPYLNGDPAAAAVTGDADGCAPSKVNSSSLVAIRKGKAWADAKGNLRI
                     TRVPAATDTTHAFAAGDVVMDPGYGSVGLCTGGGYPSGHTTTAYEAGITLATLLPELA
                     PEILTRASEAGNNRIVLGVHYALDIVGGRINGELALAARWSDKAFRTGVLEPARAELV
                     GYLQARCGARLAVCIARDKAYADNPYGGAKVPGGTSQIVTNRRSAVKVYTERLGYGFA
                     PVRSTRQSASVPATASSLLLSTFPKLTAKQRRAVLAQTEIASGHPLDTTWSSRHGTAP
                     GSWQRLNLAAAMSATVRVYRDGHVKVLSTGGQPKLIFVLR"
     gene            complement(11493..12503)
                     /locus_tag="PLF56_00445"
     CDS             complement(11493..12503)
                     /locus_tag="PLF56_00445"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_013864721.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="aldo/keto reductase family protein"
                     /protein_id="HOQ52060.1"
                     /translation="MEFRYLGRSGLKISEICYGNWLTHGSQVENEAALACVRAALEVG
                     ITTFDTADVYANTKAESVLGEALAGERRESLEIFTKVYWPTGPGGHNDVGLSRKHIME
                     SINASLRRLNTDYVDLYQAHRFDVETPLEETMQAFADVVRQGKALYIGVSEWTAEQIR
                     AGHALAGELGIQLISNQPQYSALWRVIEGEVVPTCRELGLSQVVWSPVAQGVLTGKYL
                     PGQPLPDGSRATDDKGGKNMISRFMNDTILERVQKLSPIASDLGLTMAQLAVAWVLAN
                     DNVACAIVGASRPEQVTANAEASGVTLTDDVKVAIDEALGDVVVTDPAMTAKNAPAKR
                     PA"
     gene            12740..13663
                     /locus_tag="PLF56_00450"
     CDS             12740..13663
                     /locus_tag="PLF56_00450"
                     /EC_number="2.7.1.26"
                     /EC_number="2.7.7.2"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_002518460.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_function: GO:0003919 - FMN adenylyltransferase activity
                     [Evidence IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="bifunctional riboflavin kinase/FAD synthetase"
                     /protein_id="HOQ52061.1"
                     /translation="MSETVVVIGNFDGVHPGHVEVLAEAHARRPDLPLVVVTFWPHPV
                     SVLRPEVGPMLLTGLDERIALLRQAGADRVEVIDFTPAFAQHTPQEFVEQVLLPLRPA
                     LIVVGENFRFGHRASGNVQTLRELGAGHFEVEALRLVRFGDEETCSTVIRQALASGDV
                     AHAREHLGRPFRFSGVVTHGDHRGRELGFPTANLPVPSLLACPADGVYAGWLSRLDGL
                     DAHGDPLLAGESPAQWPAAISVGTNPTFDGVQRRVESYVLDRSDLELYGAPVAVDFIT
                     RIRGQVRFEGIEALIEQMTDDVRRCHQILNT"
     gene            13873..14361
                     /locus_tag="PLF56_00455"
     CDS             13873..14361
                     /locus_tag="PLF56_00455"
                     /inference="COORDINATES: ab initio
                     prediction:GeneMarkS-2+"
                     /note="Derived by automated computational analysis using
                     gene prediction method: GeneMarkS-2+."
                     /codon_start=1
                     /transl_table=11
                     /product="hypothetical protein"
                     /protein_id="HOQ52062.1"
                     /translation="MTWLVALAVVGALMAVTVLICRPPRRTVVPFDEPVSAHTFLGLA
                     DSDARELIELGHRVEQEFGLVPVQVLLNRLGTVTRRHVPLRAIRPGSSRGLARICFAD
                     GTTLQVRGREVGDLGQLAVTALTQKVWLADYRTEQSHVVLDLDWHRGHVTVLAVGLDQ
                     AD"
     gene            complement(14521..>14953)
                     /locus_tag="PLF56_00460"
     CDS             complement(14521..>14953)
                     /locus_tag="PLF56_00460"
                     /inference="COORDINATES: protein motif:HMM:NF025054.2"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_function: GO:0008080 - N-acetyltransferase activity
                     [Evidence IEA]"
                     /codon_start=2
                     /transl_table=11
                     /product="GNAT family N-acetyltransferase"
                     /protein_id="HOQ52063.1"
                     /translation="PHANARYAQGHFERQQQGDYLFALAVRGADLLGTSVLDCRGDNL
                     LQPELKSLWVYPEHRRVGAARSLTRYLEEQALDRGFDEIFLRVDPQNAAAIPMYISLD
                     YTPTGDHHLTTYEYVDGLGASHSREQMDAIYRKSLRLLRAQ"
BASE COUNT         2204 a         5223 c         5080 g         2446 t
ORIGIN      
        1 caaggccact cagatggccg actcggccgg tgtcgacatc cggtactact cggtgatcta
       61 cgcggccatc gacgatgtgg aggcggcgct caagggcatg ctcaagccga tcttcgaaga
      121 gaaggtcagc ggccaggccc agatccgcga gatcttccgg tcgtccaagt tcggcaccat
      181 cgcgggctgc atggtgatcg acggctcgat ccggcgcaac agcaaggcgc gcctgatccg
      241 cgacggtgtc gtggtcaccg aatcgtccat cgcctcgctg cggcgtgaga aggacgacgt
      301 caccgaggtg cgcgaggggt acgagtgcgg catgacgctg gccaactact ccgacatcaa
      361 ggtcgacgac gtgatcgaga ccttcgaaat ggtcgagaag ccgagggact gagatgggta
      421 acccgcgcta catcaaggtc gccgagcaga tcaaggtgat cgtggccgaa atgctggagc
      481 ggcggatcaa ggatccccgc ctcggcttcg tcaccgtcac cgacgtgcgg ctcaccggcg
      541 atggccgcga gggcacggtg ttctacaccg tgctgggcga cgagcaggcg caggccgaca
      601 cggctgccgc cctggagtcg gcgcgcggcc tgatccgctc cacggtcggc aagcagttgg
      661 gcatgaagtt caccccgacg atcgccttcc agctggacgc cgtgcccgag accgcctcgc
      721 agatcaccga ggcgctggcc cgggccaagg ccgcggacga ggcggtggcg gcccaggcgg
      781 ccaccgcgtc ctacgcaggc gacgccgacc cgtacaagaa ggcggacgag gagcaggagt
      841 aggcgttcgg ccgctgttcg gctgaccggg tgttcatccg ttgacatggg tggggatcgg
      901 gcttcgacat cgaagcccga tccccttctc gttgttcatc acctgtctga ccggagtggg
      961 agacggttct ccactccgat cacgcgggtg gccggatcgt gacgtagccg cacatcaaag
     1021 acagagtgag ttcaggcctt gagttcgagg cccagacgca cactctcttc gaggcggcct
     1081 gaccggcgct tactccaggg acgggattcg tccgggtcag gggctagcgt ctcgccatga
     1141 cggattaccg agtcgaggcg ctcgacgcca ccacctggcc cgcgttcgag gcgctggtga
     1201 gcaagcacaa cggcatcttc ggcggctgct ggtgcatgtg gttccacgac cgtagcgaca
     1261 ccggtcccgg cgccgaaggc aacaggtcgg ccaagcagca actggtgcag cggggtgcga
     1321 cccaccacgc gctggtgatg cacggtgacc aggcggtcgg ctggtgcgag tacggcaccc
     1381 ctgccgaact gccgaacatc tatcaccgca agcagtacga cgcgagcggc atcgccccga
     1441 cgacctaccg catcacctgc ttcttcgtcg accgcgacca ccgtcgtcgc ggcgtggccg
     1501 aactggccct gaccggtgct ctcgatgcca tcgcgcgcgc cggcggtggc agtgtcgaag
     1561 ggtttccgcg gcacgacctt gacggacgca agctctcggc gtcctttctg ttcaacggca
     1621 ccctgcacat gttcgagcgt gccggcttca cccgggtcgc ggacctcgcc aagttccgct
     1681 gcatcatgcg ccgcaacgtg gacgcggccg tggcactaac ctgaccgggt gagtgacagc
     1741 ggaatcctgg tggtcgacaa gccgtccggc tggacctcgc accaggtggt cggacgctgc
     1801 cgccggctgc tcggcacccg caaggtcggg cacgccggca ccctcgatcc gatggccacc
     1861 ggggtgctgg tgatcggcgt cgaacgaggt acgcggctgc tggggcatct cgcgctcacg
     1921 accaaaacgt acgaggccac gatcagactc ggccaggcca cgaacaccga tgacgccgat
     1981 ggcgagctca ccgcggcgca gggtgcgtcc ggcatcaccg acgaggccat cgaggatgcg
     2041 ctggcggtgc tgcgcggcga catcgtgcag gtgccctcca ccgtgtcggc gatcaagatc
     2101 aacggccagc gcgcgtacgc gctggcccgc aagggcgagg acgtccgact ggccggccgg
     2161 ccggtcaccg tcagccgctt cgacgtgctg gcccgccgca ataccgaggc ggacggtgtg
     2221 ccggtcgtcg atctggacgt ggtggtcgac tgctcgtccg gaacctacat cagggccctg
     2281 gcccgcgatc tgggtgctgc gctgggtacc ggaggtcatc tgacgagcct gcgccgaacc
     2341 cgggtcggcg gcttcgacct caccggcgca ctcaggcccg atgcgctgac cgccgacccg
     2401 ccgcagcgac ccgccctgat cggtctggcc gcggtcgccc gcgaccactt cgcgttcgtc
     2461 gaactgggcg acgacgaagc ccgcgacgtc ggctacggac ggcccctgcg caggcccgtc
     2521 cccgccgacc cgaccgcgct gctgcacggg caggagttgc tggcgctgta ccggccggcg
     2581 ggggagcggg ccgtccccgt cgccgtgctc gtcggctgag tgccgaaccg agcccaacca
     2641 ccgcatcgcg cgttgatcag cgttgcctga tgccggagag cggtcaccgg tgcgttcccg
     2701 ggggatcgct ctgactgcgg gacccttgtc aagcgtcccc ccgtcgtgca gggtgtagcc
     2761 acacacccgg aggttcccat gtccgccacc gaacagaccg aaaccggcca gcccaatgag
     2821 ctgaagcggg tgatggggcc cgggttgctg ttgctgttca tcatcggcga catcctgggc
     2881 accggcatct acgcgctcac cggcgtggtc gccaaagagg tcggcggtgt ggtctggttg
     2941 ccgttcctga tcgccttcgt cgtcgccacc atcaccgcct acagctacct ggagttggtc
     3001 accaagtacc cgcaggcggc cggcgctgcg ctctacgcgc accgcgcctt cggcattcac
     3061 ttcctcacct tcatcgtcgc gttcaccgtg atgacctcgg gcatcacctc ggcatcgacc
     3121 gccgcccggg ccttcgccga gaacctgcag aagggcttcg gcctggacgc ctcggccggg
     3181 ctcaccacgg tgatcgcgat cggcttcatg ctgctggtgg cggcggtcaa cgtccgtggg
     3241 gtgggcgaga gtgtcaaggc caatgtggtg ctcacctgca tcgagctgtc cggcctgctg
     3301 atggtgatct tcgtcgccgt tttcgcgatg tccgagggcc gggccgactt ctcgcgggtg
     3361 accgtcttcc acacccccga ggacaagggc gtcttcatcg ccgtcaccac cgccacctcg
     3421 ctagcgttct tcgcgatggt cggcttcgag gactcggtga acatggccga agagaccaag
     3481 gagccgtcca ggatcttccc gaagatcatg ctgaccggtc tgggcatcac cggggtcatc
     3541 tacgtgctgg tgtccatctc ggcggtggcg ctggtgcccc cgggcgagct gtccaaagag
     3601 ggcgcgacac cgctgctgct ggtcgtgcag accggtgccc ccgacctgcc gatcggcgcc
     3661 atcttcccgt tcatctcgat gttcgcggtg gccaactcgg cgctgatcaa catgctgatg
     3721 gcctcgcggc tgctctacgg catggccaac gagggggtcc tgccacccgc cttcggccgg
     3781 gtgctcaagg ggcgccggac gccgtgggtc gccattctct tcaccaccgc gctggcggtc
     3841 ggcctgatca ccttcgtcgg ccaggtgtcc caactggggg gcaccaccgc gctgttgctg
     3901 ctcggcgtgt tcaccctggt gaacatctcc gtgctggtgc tgcgcaagga cccggtcgaa
     3961 cacgaccact tcaccgcacc cacctggctg ccgatcgtcg gtgcgctcag ttgcgcctac
     4021 ctggtcgggc cgtggaccgg acgctccctc gagcagtact ggatcggtgc cgtgctgatc
     4081 ggaatcggcg tggtgctcag cctggtgatg ttcctggtca accgtcgccg gcacaagccg
     4141 acccgcttcg aacaccccga cgacgacctg cgcagtttcg ggccgctcaa ctgacgcctc
     4201 gagtgatttc cccatagcca atcgggtggg ccgttatcaa gcctgcccag agtacggacc
     4261 cgggctcgcc actaggcacg cgagtaaata agttatgagt gtgtcaacaa ataaatctcc
     4321 ccaacagcac ctgtccgttc acacccgcac ccgtcgcgcc ctgcccggtg cgttgatggc
     4381 cgtggtcgcg ctgctgacca ccgcatgcag cgcgggcacg cccgcggcgt ccaccccggc
     4441 cggaccgacc gtggtgatcg agaacgccaa gcccaccttg aagcaggtcg aggccgacgg
     4501 aaccacctcg atgcaggaga acctggagat cgcccgagcc gcggtcaccg tgccgcagaa
     4561 gcccaccaag gtcgtcacct tcgacatcgc caccctcgac accctggacg ccatcggcgc
     4621 cggtgacagc gtggtcggcg tgcccgccat cgggctgccc gactacctcg tcaagtacgc
     4681 cgacctgccc aaggtgggca ccctgttcga gcccgatttc gaggccgtct ccaagctcgg
     4741 ccccgacctg atcgtcaccg ccgcacgcag taccggtgcc tacgacgaac tgtcccggat
     4801 cggcatcacg atcgatctga ccgcgaagta cgccggcgcc ttcgaccctg acgcagcact
     4861 ggtgcgagcc aagcaactcg gcacgatctt cgacaagaac gacgaggtca ccaagctcgc
     4921 cgacgcgatc accgccaagc agcagagcct gcaggccgag gccaagaagg ccggcagcgc
     4981 gctgctgctg tcggtctcgg gtggcgagta cggcgccttc ggcgaaggct cgcggttcgg
     5041 ctacttcttc gacaccctcg gcttcacccc ggccgtgccg gcagccgagc ttcccggcgc
     5101 gaaaggctcc tcacacggcg acaccgtctc gaacgagttc atcctgaagg ccaaccccga
     5161 ctggatcatc gcgttcgacc ggggtgcggc caccggtgag ccggccagcg ccgagcagac
     5221 cctgaccaac gaactggtcg cgaagaccac cgccggccag aacaagcaga tcatctacct
     5281 gccggcgtcc gagctgtaca tcgtgatcaa cgggctcact gccatcgatc gggtgctcga
     5341 ctcggtacac ggcgcgctga ccacatgagc cggtgctgac ccgtcccacc ctgctgatca
     5401 tcgcgggggt cgtgctggtc acggccctcg cgacggcgtc cgtgctggtc ggcgtggccg
     5461 agctggacgg cttcgtcctg ctggccagcc ggctgccgcg caccgtcgcc ctcgtgctga
     5521 ccggggcggc gatggccgtc accggcctga tcatgcagct gctgacccac aaccggttcg
     5581 tcgaacccac caccaccgga gccggcgacg ccgccagctt cgccctgctg atgctgctgc
     5641 tgaccaaccc gggcctgccg ttgcccggca aggccgccgt cgcgacgctg ggcgccctgg
     5701 tcggcgtctt cgggttcctc accctggccc gccgcatccc cgtccgctcg agcgtgctgg
     5761 tgccggtcgt cggcatcatg tacggcggcg tgatcggcgc cgtcaccacc ttcatcgcct
     5821 accgcaccga gctgctgcag gaactgctga gctggagcat cggcgacttc tccggcattg
     5881 tgcgcggccg ttacgagatg ctctacctcg ccgcggccgc cgccctggtc gcctggatcg
     5941 ccgccgaccg gttcaccgtc gccgcgctcg gcgacgacac cgcccgcggc ctcggcctgg
     6001 acagccgcgg cgtgctgctg ctcggcgtca ccatcgtcgc cgtggtcacc ggcatcgtgt
     6061 cggtcaccac ggggttggtg ccgttcctgg gcctggtggt gcccaacctg gtcagcaggc
     6121 tgatcggcga tcacatgcgg cgcgctgtgc cgctggtggc gttgctcggg gctgcactgg
     6181 tgctggcctg cgacctggtc ggacgtctcg tccggttccc gttcgaaatt ccgatctcga
     6241 tcgtgatggg catcgtcggc ggcaccgtgt tcctgtggct gctgctcggt ccagcctcgc
     6301 ggacgggcgg tgcaacgcgg agcggcaacg cttcgaaggc cggccgcgca acgggtactg
     6361 ccgcccgccg ggccgaggcg gtgggacgac gtggctgaac ccaccgtcac cgaacgtggc
     6421 acccgtcccg ccgggggcac cacatctcac gcgcccgccc ggcggcgggt ccggttcggc
     6481 ggcggtgcgc ggatcgggct gctcggcgca ctggccgccg gtgcggtcgc gttgtatctg
     6541 ttccacgacc tgcccgaacg ctggcagtac ccgatggacc tgcgcacccg caccgtcgcc
     6601 gccatgctgc tggtcgcggt cgccgtcggc gtctcgacgg tgctgttcca gaccatcacc
     6661 gccaaccgca tcctgacccc cggcatcatg ggcttcgacg cggtcttcat ggcgattcag
     6721 gtgttcaccg tgttcgcgct cggcccgatg gtgctgctcg ccgcgccccc gctggtgtcg
     6781 tggctggtcg aactcgtgct gatgtcgggc ttcgtggtgg cgctgtattg gggcctgttc
     6841 ctgggccgcc ggctcgacct gcacgtgatc gtgctggcgg gcctggtgct gggcaccgtg
     6901 ttccggtcgg tgaccgcctt tctgcagcgg ctgctcgacc ccgacacgtt cgccatcgtg
     6961 cagaacctcg ccttcgccag cttcacctcg gtcgaccgga cgctgctgct gccgacggcg
     7021 ctgctggtgc tggccgcgct ggccagcctg tggccgatcc gccggtcact ggacgtgctg
     7081 tccctcggcg aggccccggc catcgggctg ggcgtggagc atcgccgggt ggtgatgcac
     7141 gtgatcgtgg tggtcgcgct gatggtcggg gcgtccaccg cgctggtcgg cccggtgatg
     7201 ttcttcggcc tgctggtcgc caacgtcgcc tactccgtgg tcggacaccg gcacagtcgg
     7261 gcgctgcccg cggcgtgcct gatcgggctg atcgccctgg tcggcgggca gtggtttctc
     7321 gaccgggtgc tcggcttcgc caccgagctg ccggtcgtga tcgagtttct cggcggggtc
     7381 gtgttcatcg tcctcgtcct gaccggaagg gcccggtgag ccgatgatcc gcaccgaagc
     7441 gctgaccaag cggcacggcg agatcgtcgc cgtcgacgac gtgacgatcg agctcggcga
     7501 cgggttgacc gccatcatcg gccccaacgg cgccggcaag tcgacgttgc tgtgcgcgat
     7561 cggccggctg atccgcgctg atgccggacg cgtcgtggtc ggcggcctgg acgtcgccac
     7621 cacccgctcg cgcgagctgg ccaagcggct ggcggtgctg cgtcaggaga accacctgcc
     7681 gatccggctg agcgtgcagg aactggtcgc cttcggccgc tttccgcacg gcggcaccgg
     7741 acgtagcccg cgtgaccgca cccacatcga gggggcgctc gacctgctcg gcctggacga
     7801 ggtgcgcgac cgctttctgg acgagctgtc cggcggccag cggcagcggg ccttcgtggc
     7861 gatggccctc gcgcaggaca ccgactacct gctgctcgac gagccgctga acaacctcga
     7921 cccgcggcac gcggtcgagc tgatgcggct gctgaccggg ctggcggccg agcgcgggct
     7981 gcggatcgtg gtggtgctgc acgacctcaa cgtggccgcc cgctacgccg acgacgtggt
     8041 ggccatgcgc gaagggcgag tggtcgccca gggcgccgtc gagcaggtga tcaccgtcga
     8101 gcagctcacc gacctgtacg acacgccggc ccacgtcgtc gaggtcgagg gccgccgggt
     8161 ggtgctgtgg cagtagctgc gcgtgggtgg tagttgtgcg cagtctcggc cacagcgacg
     8221 tccgctcttg agcagcaggt ccgaacacgc gggcccaacc cctaggaggc agacatgccc
     8281 aatgcagaac tggacgccaa gctggccggc aacttcgccc gcgccatcga cttcaaggcg
     8341 gtcggtgaga cggtcgccga tgtcggtggc gcgctgtacg acgacgacgg ctggatccgc
     8401 tggtgcggca gcgacttccg catcttcaag tggcgcggct tcgaactgca cgacgaactg
     8461 atcaaggagc tcacgatcaa cctgcgcgag ctgaacgcca agctgggcga gggccggatc
     8521 ggatgacatc cactctggcg gctccggccg ggtcactcac ccggccggcg ccgtccgtca
     8581 tcgacgcgcg ctggatcgag cgcggcgtgg gcgtctgcga gcgggtcctc gatgtggtgc
     8641 tgaccacccg ggaccagtcc gccgtggccg aggtcggtgc cagcatcgcc aaggtggttg
     8701 ccgaatcggc catcctgctg cgcatcttga agccctggca ccactgcgta ctggccgccc
     8761 ggctcgccga gcgactcgcc gtcctggccg tcgacccgcg cacgctgacc gcgctcgcgc
     8821 tgcacgcgtc cgccgcgttc gagctggcgg tgccctacct cgtgctgcgc gaacggcacc
     8881 gcgacgaccg ggtggacgat ctgctccaca ccgccttcgc cgcccgtgcc gcgcagggcc
     8941 gcgagttgct gccgcatcgg gctctggagc ggctctggct gctcggcctg ctgacgggac
     9001 gccgcgagtt cacccgcacc gacctcgagg ccaccgtgct cgcccgcgag atcgacctgc
     9061 tgcacggcac ccgcgacgat cattacgcct tcacccacgc cctgatgtac gccaccgact
     9121 tcggacgcga tcccgacgcc gttcctgcgg actggaagcc tcggctggtc gaccaggccc
     9181 gctccgccct ggccggggcg ctcgatgagg acgactatga cctggccggt gaacttctgc
     9241 tcacctggcc gttccttcgg gaaaccgccg ggccggtcga gtccaccgcg ctgatggtgc
     9301 tctgcgacgt ggcggacgcg gtcggaacgc tgccgtccct caccctcgcg ctggtcgagg
     9361 gcaccgacag actgccggcg gccgacgcgg tggtgctgtc gacgtaccac accagcttcg
     9421 tcttcaccgc gctggcggcg ctcgccccgc gggtcgaatg gcggacgtcc gcggcgaacg
     9481 gcccgagcct gcgtgccacg tggccctccg gcggacgtcg tccgggctgg ctggacgtgc
     9541 ccgcggccgg cttgcctgcg ggcccgctgg gcctggaggc aggtgtgcgc cgcgccgtcc
     9601 gcgagcaccg gttggccgac gtcgcggccc tgctcagcgc accgggcacc ccggccacgc
     9661 cggccacgaa ccaggccctg caactgctcg aacggctctc gctgctgccc tgatcggcga
     9721 tggtggccgc gctgccaacg gaaggtgacg cgcgacccct tcgggggtga agccttgccg
     9781 accgtcggcg gatcggtgca gcgtggggga acgtgagggg cgacgatgtc tgagtcggca
     9841 cgcctccgcg gctgtccggt gcccacccgg accacccggc gggctgcctg ccgcgccccg
     9901 gcgtccgccc gggcattagt caggaaggcg atacacccca tgaccccacc cattgacggc
     9961 cggctccgcc gtggccgcgc gctcgctgcg accgcggcca ccctcgccct gctcgcctcg
    10021 accggcctga cgaatgcgca ggccgccacg tcctacccct ccgacaccgc caagccggat
    10081 ctgatgcccg cactgagcgg ctacagcgac ctgtggcagt cgtccggact gaacgacctg
    10141 cacggcaccg tcaagaactc caccgtgttg cagtggaacg accgggtgac cagctggatc
    10201 aaccagcacg cgacggcgaa gcagcagttc cgcgccctgc agaactccaa ctatctggcc
    10261 tcggacggct ccggctacga ccagtcgatc agcatcgccg acgggctggg caagaagctc
    10321 ggcgcactct acgcccaggg ccggatcgag aagaagctgc cgctggtcgc ggccctgatc
    10381 aacagttcga ccggcgccac gggtgcctat gtcagtaccg gcgccgcgaa ggccgccttc
    10441 agctacccgc gtccgtacct caacggcgac ccggccgcgg ccgcggtgac cggtgacgcc
    10501 gacggctgcg cgcccagcaa ggtgaacagc tcatcgctgg tcgcgatccg caagggcaag
    10561 gcgtgggcgg acgcgaaggg caacctgcgg atcacccggg tgccggccgc caccgacacc
    10621 acgcatgcct ttgcggccgg cgacgtggtg atggaccccg gctacggttc ggtcgggctc
    10681 tgcaccggcg gcggctaccc gagcggtcac accacgaccg cctacgaggc ggggatcacc
    10741 ctggccacct tgttgcccga gctcgcgccc gagattctca cccgcgcctc cgaggccggc
    10801 aacaaccgga tcgtgctcgg cgtccactat gccctcgaca tcgtcggcgg ccggatcaac
    10861 ggcgagttgg cgctggccgc acgctggtcc gacaaggcct tccgcaccgg tgtgctggag
    10921 cccgcccgcg ccgagctggt cggctatctg caggcccggt gcggcgccag gctggcggtc
    10981 tgcatcgcca gggacaaggc gtacgccgac aatccgtacg gcggcgcgaa ggtgccgggg
    11041 ggtaccagcc agatcgtcac caaccgcagg tcggcggtga aggtgtacac cgaacggctg
    11101 ggttacgggt tcgcgcccgt gcggtcgacc cgtcagtccg cctcggtgcc ggccaccgcg
    11161 tccagcctgc tgctgagcac ctttccgaag ctgaccgcga agcagcgcag ggcggtgctg
    11221 gcccagaccg aaatcgcttc cggacatccg ctggacacca cctggagcag ccgccacggc
    11281 actgcgccgg ggtcgtggca gcggctcaac ctggccgcgg ccatgtcggc gacggtgcgg
    11341 gtgtaccgcg acggccacgt gaaggtgctc agcaccggcg ggcagccgaa gctgatcttc
    11401 gtgctgcgct gaccagcggt gatcgggccc gccggcactt cttcggaaac cggcgggccc
    11461 gatccttgct gtctgactac tgccgcggag gctcaggccg gacgcttggc cggcgcgttc
    11521 ttcgcggtca tcgccggatc ggtgaccacc acgtcgccga gtgcctcgtc gatcgccacc
    11581 ttgacgtcgt cggtcagcgt cacgcccgac gcctcggcgt tggcagtgac ctgctcggga
    11641 cgcgaggcgc cgacgatggc gcacgccacg ttgtcgttgg ccagcaccca ggcgacggcc
    11701 agttgcgcca tggtgaggcc cagatcggac gcgatcggcg acagcttctg cacccgttcg
    11761 agaatggtgt cgttcatgaa ccggctgatc atgttcttgc cacccttgtc atcggtggcc
    11821 cgcgacccgt ccggcaacgg ctggccgggc aggtacttgc cggtcagcac gccctgcgcc
    11881 accggagacc acaccacctg cgacaggccg agctcgcggc aggtgggcac cacctcgccc
    11941 tcgatcaccc gccacagcgc cgagtactgc ggctggttcg agatcagctg aatgcccagc
    12001 tcgccggcca gcgcgtgacc ggcgcggatc tgctcggcgg tccattcgct caccccgatg
    12061 tagagcgcct tgccctgccg gacgacgtcg gcgaacgcct gcatggtctc ttcgagcggg
    12121 gtttcgacgt cgaatcggtg tgcctggtac aggtcgacgt agtcggtgtt gagccgacgc
    12181 agcgaggcgt tgatcgactc catgatgtgc ttgcggctga ggccgacgtc gttgtgcccg
    12241 ccgggaccgg tgggccagta caccttggtg aagatttcga gcgactcgcg gcgttcgccg
    12301 gccagggcct cacccagcac gctttcggcc ttggtgttgg cgtagacgtc ggcggtgtcg
    12361 aacgtggtga tgcccacctc aagcgcggcc cgcacgcacg cgagcgcagc ctcgttctcg
    12421 acctgtgacc cgtgcgtcag ccagttgccg taacagattt cggagatctt caaccccgag
    12481 cgacccagat agcggaattc catgggtcca tccaaccacc tcggtccgac acgaccagtg
    12541 acaacgaagc agctggccgg gtagggggcc ctgccatcga cgcgtggtac cggcgagcag
    12601 cgttcgggcg aatgcgtcgt gaggccgacg aggaagccgt accgggtcgt gtggtgaccg
    12661 aggagaacgc cgcgacgcac cgtccgggcg tcgcccagcg ggcaaaggtg ggtggccggg
    12721 ggcactaggg tggtccaccg tgagcgaaac ggtggtggtc atcggcaact tcgatggcgt
    12781 gcaccccggg cacgtcgagg tgctggccga ggcgcacgcc cgtcgtccgg accttccgct
    12841 ggtcgtggtc acgttctggc cgcacccggt cagcgtgctg cggcccgagg tgggcccgat
    12901 gctgctgacc ggcctggacg agcgcatcgc gctgctcagg caggcggggg ccgaccgggt
    12961 cgaggtgatc gacttcaccc ccgctttcgc acagcacacc ccgcaggagt tcgtcgagca
    13021 ggtgctgctg ccccttcgtc cggcgctgat cgtggtgggg gagaacttcc gattcgggca
    13081 tcgggcgtcc ggcaacgtcc aaaccctgcg cgagttgggt gctggacact tcgaggtcga
    13141 ggctcttcgg ttggtgcggt tcggcgacga agagacgtgt tcgacggtga tccgtcaggc
    13201 tctggccagt ggcgacgtcg cgcatgcgcg tgaacacctc ggtcgtccgt tcagattcag
    13261 cggcgtggtc acccacggtg atcatcgcgg ccgcgaactg ggcttcccga cggcgaacct
    13321 gcccgtgccc agcctgctcg cctgccccgc ggacggcgtg tacgcgggtt ggctcagccg
    13381 gctggacggc ctcgatgcgc atggcgaccc gctgttggcg ggggagagtc cggcgcaatg
    13441 gcccgccgcc atctcggtcg gcaccaaccc gaccttcgac ggcgtccagc gccgggtcga
    13501 gtcgtacgtg ctggaccgct ccgacctcga gttgtacggg gcgccggtcg cggtcgactt
    13561 catcacccgg attcgcggtc aggtgcgttt cgagggcatc gaagcgctga tcgagcagat
    13621 gacggacgac gtgcgtcgct gccatcagat tctgaacacc tgaacgggcc tacgggctgc
    13681 gccctcgagc ggcaccatgt gtggccgacc acgcactcat cgacggcgcg caacgcgaca
    13741 ttcgatggcc tccagcgccg ggtcgagtca tacgtgctgg accgctccga cctcgagttg
    13801 tacggggcgc cggtcgcggt ccacgtgata aagggtcttg cgtgcggccg gacgcgggcg
    13861 cacagtagct gcatgacgtg gctggtggcc ctggcggtag tgggcgctct gatggcggtc
    13921 accgttctga tctgccgccc gccgcggcgc accgtggtgc ctttcgacga accggtgtcc
    13981 gctcatacgt ttctcggcct ggccgattcg gacgcccgcg agctgatcga gttggggcac
    14041 cgggtcgagc aggagttcgg cctggtgccg gtgcaggttc tactgaatcg gctcggcacc
    14101 gtcacccggc gtcacgtgcc gttgcgtgcc atccggccgg ggtcctcgcg gggactggca
    14161 cgcatctgct tcgcagacgg caccaccctg caggttcgtg gccgtgaggt cggcgatctg
    14221 ggccagctgg cggtgaccgc gctcacccag aaggtgtggc tcgcggatta ccgcaccgag
    14281 cagagtcatg tggtgctcga cctcgactgg caccggggtc acgtcaccgt tctcgcggtc
    14341 gggctcgacc aggcagactg agccccgtcg gtcagcggac gtgccccgcg ccagagcggc
    14401 cttcggggca gtgctgatca ggcgcgacca gttggtcgag cttgtcgaaa atcctgttcg
    14461 catgggtctc gaccagggag agatcgacca ggaagagatc ggcgcttcct tggggctgaa
    14521 ctactgagcc cgcagcagtc gcagcgactt gcggtagatg gcgtccatct gctcgcgcga
    14581 atggctggcg cccagcccgt ccacgtattc gtaggtggtc aggtgatggt cgccggtcgg
    14641 ggtgtagtcg agcgagatgt acatcgggat cgccgccgcg ttctgtgggt cgacgcgcag
    14701 aaagatctcg tcgaagcccc ggtcgagcgc ctgctcttcg aggtagcggg tgagcgacct
    14761 ggccgcgccg actcgacgat gctcggggta gacccacagg ctcttcagtt cgggctgcag
    14821 caggttgtcg ccacggcagt cgagcacgct ggtgccgagc agatcggcac cgcgaaccgc
    14881 aagtgcgaag aggtagtcac cctgctgctg tcgttcgaaa tgtccctggg cgtagcgggc
    14941 attcgcgtgc ggc
//
LOCUS       DAOSUG010000007        14555 bp    DNA     linear   ENV 10-JUN-2024
DEFINITION  MAG TPA_asm: Micropruina sp. isolate AMR_MDS_2195
            NODE_2498_length_14555_cov_4.979241, whole genome shotgun sequence.
ACCESSION   DAOSUG010000007 DAOSUG010000000
VERSION     DAOSUG010000007.1
DBLINK      BioProject: PRJNA925477
            BioSample: SAMN32864262
            Sequence Read Archive: SRR4236673
KEYWORDS    WGS; ENV; Metagenome Assembled Genome; MAG; Third Party Data; TPA;
            TPA:assembly.
SOURCE      Micropruina sp. (wastewater metagenome)
  ORGANISM  Micropruina sp.
            Bacteria; Bacillati; Actinomycetota; Actinomycetes;
            Propionibacteriales; Nocardioidaceae; Micropruina.
REFERENCE   1  (bases 1 to 14555)
  AUTHORS   Abdulkadir,N., Saraiva,J.P., Zhang,J., Stolte,S., Gillor,O.,
            Harms,H. and Rocha,U.
  TITLE     Genome-centric analyses of 165 metagenomes show that mobile genetic
            elements are crucial for the transmission of antimicrobial
            resistance genes to pathogens in activated sludge and wastewater
  JOURNAL   Microbiol Spectr 12 (3), e0291823 (2024)
   PUBMED   38289113
REFERENCE   2  (bases 1 to 14555)
  AUTHORS   Abdulkadir,N.
  TITLE     Direct Submission
  JOURNAL   Submitted (24-MAR-2023) Environmental Microbiology, Helmholtz
            Center for Environmental Research -UFZ, Permoserstr 15, Leipzig
            04318, Germany
COMMENT     The annotation was added by the NCBI Prokaryotic Genome Annotation
            Pipeline (PGAP). Information about PGAP can be found here:
            https://www.ncbi.nlm.nih.gov/genome/annotation_prok/
            
            ##Genome-Assembly-Data-START##
            Assembly Method        :: SPAdes v. 3.11.1
            Genome Representation  :: Full
            Expected Final Version :: Yes
            Genome Coverage        :: Not Applicable
            Sequencing Technology  :: Illumina MiSeq
            ##Genome-Assembly-Data-END##
            
            ##Genome-Annotation-Data-START##
            Annotation Provider               :: NCBI
            Annotation Date                   :: 05/04/2023 04:57:10
            Annotation Pipeline               :: NCBI Prokaryotic Genome
                                                 Annotation Pipeline (PGAP)
            Annotation Method                 :: Best-placed reference protein
                                                 set; GeneMarkS-2+
            Annotation Software revision      :: 6.5
            Features Annotated                :: Gene; CDS; rRNA; tRNA; ncRNA
            Genes (total)                     :: 2,776
            CDSs (total)                      :: 2,750
            Genes (coding)                    :: 2,714
            CDSs (with protein)               :: 2,714
            Genes (RNA)                       :: 26
            tRNAs                             :: 24
            ncRNAs                            :: 2
            Pseudo Genes (total)              :: 36
            CDSs (without protein)            :: 36
            Pseudo Genes (ambiguous residues) :: 0 of 36
            Pseudo Genes (frameshifted)       :: 2 of 36
            Pseudo Genes (incomplete)         :: 32 of 36
            Pseudo Genes (internal stop)      :: 2 of 36
            ##Genome-Annotation-Data-END##
FEATURES             Location/Qualifiers
     source          1..14555
                     /organism="Micropruina sp."
                     /mol_type="genomic DNA"
                     /submitter_seqid="NODE_2498_length_14555_cov_4.979241"
                     /isolate="AMR_MDS_2195"
                     /isolation_source="Activated sludge of municipal
                     wastewater treatment plants"
                     /db_xref="taxon:2737536"
                     /environmental_sample
                     /geo_loc_name="Canada"
                     /lat_lon="49.25 N 123.1 W"
                     /collection_date="2015-06-16"
                     /metagenome_source="wastewater metagenome"
                     /note="metagenomic"
     gene            complement(<1..351)
                     /locus_tag="PLF56_00465"
     CDS             complement(<1..351)
                     /locus_tag="PLF56_00465"
                     /inference="COORDINATES: protein motif:HMM:NF037017.2"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="Ig-like domain-containing protein"
                     /protein_id="HOQ52064.1"
                     /translation="MADGATGVSVLDPVTVRVTGGELGQVAMTNPDGVTVDGGLGADQ
                     QSWSTNEPLGYGRTYWLTATGTDPAGLSTTTEVSFTTVQPNGTIFPSFEPPPDRGQVG
                     VGQPLSVIFDQPPPD"
     gene            365..658
                     /locus_tag="PLF56_00470"
     CDS             365..658
                     /locus_tag="PLF56_00470"
                     /inference="COORDINATES: ab initio
                     prediction:GeneMarkS-2+"
                     /note="Derived by automated computational analysis using
                     gene prediction method: GeneMarkS-2+."
                     /codon_start=1
                     /transl_table=11
                     /product="hypothetical protein"
                     /protein_id="HOQ52065.1"
                     /translation="MAVLARGDDDADAPPPVIDGPASADAGGTAAVTFVDVSAVLSAP
                     VAEQAPTSRTTAVTNPARRAGPPTAGRSLATTSTPHPHDATDHQSPTPGYYLL"
     gene            718..1668
                     /gene="lgt"
                     /locus_tag="PLF56_00475"
     CDS             718..1668
                     /gene="lgt"
                     /locus_tag="PLF56_00475"
                     /EC_number="2.5.1.145"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_007078032.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_component: GO:0016020 - membrane [Evidence IEA];
                     GO_function: GO:0008961 -
                     phosphatidylglycerol-prolipoprotein diacylglyceryl
                     transferase activity [Evidence IEA];
                     GO_process: GO:0009249 - protein lipoylation [Evidence
                     IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="prolipoprotein diacylglyceryl transferase"
                     /protein_id="HOQ52066.1"
                     /translation="MPTPLAAAIPSPPQGVWYVGPVALRAYAIFIIIGIVVAVWWGNK
                     RFVGRGGRPGRVTDIAVFAVPFGIVGGRLYHVITDNQLYFGEGRNPWNALAIWNGGLG
                     IWGAIALGGFGAWLGCRYYKVPLAAFADAVAPGIAVAQAIGRLGNYFNQELFGAPTTL
                     PWGLDVFVRTPGGVAGVIPANGNCEFPTEYIKATPEVLCGIYQPTFLYESLWCLGVAA
                     LVVWADKRFILGGGRAFALYVAGYTLGRVWVEMLRIDPANHFLGLRINVFTSILVFLG
                     AVLFLYLRRNVSREDPAVVRGPTRRGEAGQHAGGVSAEGR"
     gene            complement(1747..2676)
                     /locus_tag="PLF56_00480"
     CDS             complement(1747..2676)
                     /locus_tag="PLF56_00480"
                     /inference="COORDINATES: protein motif:HMM:NF013594.2"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="M56 family metallopeptidase"
                     /protein_id="HOQ52067.1"
                     /translation="MIIALALLLGAVLAATAARRPLVGLLAAKRDPRVGIVAWMGCVT
                     GVLVAGVAGVVLLALPDHGGLGGLLAVCFGVLSHAAVPAWDEGVAAVGVVALVGLILR
                     GAVVARRQAVRRRQERDRFRFLVALAGLADTVDARVMWLDDPRPVAFSIGGRRGLIAA
                     SYGLWASLDPVAVSATLAHERAHLRGRHQLMVDAADALAAAFPWVPLFRAAPPALREL
                     VEAAADRAAVRRHGVDAVRTALTVLSEVPLPHPGLGMGDTAIDRRLDRLIVGRGTWAV
                     VRFGRCVAAALTVVLLPTVAALAMLLSVSCVGR"
     gene            complement(2673..3035)
                     /locus_tag="PLF56_00485"
     CDS             complement(2673..3035)
                     /locus_tag="PLF56_00485"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_009080065.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_function: GO:0003677 - DNA binding [Evidence IEA];
                     GO_process: GO:0045892 - negative regulation of
                     DNA-templated transcription [Evidence IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="BlaI/MecI/CopY family transcriptional regulator"
                     /protein_id="HOQ52068.1"
                     /translation="MRRLGELEATVMDVLWSGDGPMSVRDVRDVLGADRRLAYTTVMT
                     VLDNLHRKGYVERVMRGRAYQYRPAFTRSRASAEAMREILQGAGDREAALLHFVTSVS
                     DAESELLRSALERRDRRK"
     gene            complement(3060..3557)
                     /locus_tag="PLF56_00490"
     CDS             complement(3060..3557)
                     /locus_tag="PLF56_00490"
                     /inference="COORDINATES: ab initio
                     prediction:GeneMarkS-2+"
                     /note="Derived by automated computational analysis using
                     gene prediction method: GeneMarkS-2+."
                     /codon_start=1
                     /transl_table=11
                     /product="hypothetical protein"
                     /protein_id="HOQ52069.1"
                     /translation="MAAIALVIGVPTALVPTSVFGRMTPAPWWSYPVWMTVSVLVGLA
                     AVSGAAARPTRGRGGLVAAGAMTVLAVGCPLCNKLVLAVLGSSGALAVWAPLQPVLAL
                     AALAIAARACWWASSGERTGRRGPHKSDFGGGQGAPVKSGAIRSTGVADRVYPAGRPR
                     AAAER"
     gene            3741..4004
                     /locus_tag="PLF56_00495"
     CDS             3741..4004
                     /locus_tag="PLF56_00495"
                     /inference="COORDINATES: ab initio
                     prediction:GeneMarkS-2+"
                     /note="Derived by automated computational analysis using
                     gene prediction method: GeneMarkS-2+."
                     /codon_start=1
                     /transl_table=11
                     /product="hypothetical protein"
                     /protein_id="HOQ52070.1"
                     /translation="MSPKKRVRARTEPAPGRTDRAAQHWARRRRRRIAAGGLAGLAIV
                     VLVSHWLEHLGLLRIMTPAAQDLFVGYPTAFLLLIIAGILWGV"
     gene            complement(4063..5712)
                     /locus_tag="PLF56_00500"
     CDS             complement(4063..5712)
                     /locus_tag="PLF56_00500"
                     /inference="COORDINATES: protein motif:HMM:NF013073.2"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="NlpC/P60 family protein"
                     /protein_id="HOQ52071.1"
                     /translation="MTALLALAPLTSVPAAAVPPPPPNPSDDELNASRDQATAAAAEA
                     GRLAGLVARTEGDIQRLQDRMELQMELANKAAVDLQVAQSDAAAAQAAATSAQSELDA
                     AGQAVEAAEGDAAAFAAASFRQGSVLGSMTALLDAGSPTDLLGRRELLDQMSGSVLNA
                     VDRLHAQRIRQANLTSEAGAARDNAAITQTRAEQAKATADQAAVDADQALGAGQAELA
                     VLQGRLEQQQSDYAAALAAVDGLQQQREQFQQWLAQKQAEEEAARRAAEEAARKAAEE
                     AARQAAERAAAEEAARQATERAAAEEAARQAAEEAARQVAERAAAEEAARIAEEQATT
                     ARAAQVALTAEPFYATCDAARAAGRGPITTDEPGYRPELDPNDNGIACEGPANPPASA
                     APAPAPVQDSGRTSDRGQRVVDAALRWLGTTYAWGGGNASGPTLGIRDGGVADRFGDY
                     KKIGFDCSGLTLNAWAQVGVSLPHYSAYQYAGQPKIGRDELQAGDLVFWAYNTSDPTT
                     IHHVAIWMGNGQIVEARNSGTVVKVSPMNWDGYIGAVRPGG"
     gene            5968..6381
                     /locus_tag="PLF56_00505"
     CDS             5968..6381
                     /locus_tag="PLF56_00505"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_008359916.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="four-helix bundle copper-binding protein"
                     /protein_id="HOQ52072.1"
                     /translation="MSLPAQMLDSHPAAAFATANQAIAECIQACVECAQACIACADAC
                     LAETMVADLVRCITSDLNCADICESTGRVLTRQAGADRAMIFRMLQSCAAACKACGDE
                     CETHSQVHAHCRLCAAACRRCEQARRDLLEALPIG"
     gene            complement(6457..8979)
                     /locus_tag="PLF56_00510"
     CDS             complement(6457..8979)
                     /locus_tag="PLF56_00510"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_008363391.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_function: GO:0042625 - ATPase-coupled ion transmembrane
                     transporter activity [Evidence IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="heavy metal translocating P-type ATPase"
                     /protein_id="HOQ52073.1"
                     /translation="MTSADRTVVVEVSGVHWASSKAVTEAVLGRRPGVHAVDANPVSQ
                     TATVTYDPTRTTVADLRGWIRDCGYHCSGQSVPDHICDPLAEPPGQSAHGQAAAATHA
                     GHGAPSVIAEPADHAEHGAHTAMTHVSATSADHAGPGHDHAPGRSPQEVMGHGGHGHH
                     GGGMSMDAMVRDMRNRFLVAAVLSVPILLWSPIGREVLGFTLPAPFGLRDDVFSLILS
                     LPVVFYSAWIFFDGAYRALRARTLDMMVLVAVAVAAGWLYSVGVTLTGGGEVFYEAAT
                     VLTAFVLLGHWFEMRARGGANDAIRTLLELAPPKAIVIRDGREVEVPTSEVAVGDLLL
                     VRPGAKIPVDGAVEDGESEVDESMVTGESLPVPKQPGSEVIGASVNVSGSLRVRATKV
                     GADTALAQIVALVQEAQNSKAPGQRLADRAAFWLVLVALIGGTLTFAVWLLAGAGVQT
                     ALLFAITVVVITCPDALGLATPTAIMVGTGLGAKRGVLFKNATALETAARVDTVVMDK
                     TGTLTRGEPEVTDVITADHQDTLRVLALVAAVERESEHPLAAAVVRHADGRQAPRLAA
                     TGFRSVAGHGALADVDGQRVIVGNRKLMAELDVDLGGLAARRDELADTGRTAVFAAVD
                     GQAVAVIGMADAPRDTASAAVAALHEAGVRVVMLTGDNQATARRIADQLGIDEVIAEV
                     LPGDKAAKVAELQQTGRRVAMVGDGVNDAPALAQADVGIAIGAGTDVAIETADIVLMR
                     SDPLDVPTALRIGRGTLRKMRQNLGWAIGYNTIALPIAAGVFMPAFGLMLRPEIAALS
                     MSGSSLIVAVNALLLKRLRLPDPVPPSGQRRIDAAAPVPTSA"
     gene            complement(9020..11353)
                     /locus_tag="PLF56_00515"
     CDS             complement(9020..11353)
                     /locus_tag="PLF56_00515"
                     /EC_number="7.2.2.-"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_020391618.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_component: GO:0016020 - membrane [Evidence IEA];
                     GO_function: GO:0005524 - ATP binding [Evidence IEA];
                     GO_function: GO:0019829 - ATPase-coupled monoatomic cation
                     transmembrane transporter activity [Evidence IEA];
                     GO_process: GO:0006812 - monoatomic cation transport
                     [Evidence IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="heavy metal translocating P-type ATPase"
                     /protein_id="HOQ52074.1"
                     /translation="MTEHAVDLQIGGMTCASCAARIEKKLNRMPGVQASVNYATEKAH
                     VVLPEGTEVAAAIATVEATGYTATVPAPPRPPDTGAATRGPAEADEDPEVASLRRRLV
                     VSTALTVPVLLLAMVAPLQFDNWQWLSLTLAAPVVVWGAWPFHRAAWTNLRHGAATMD
                     TLISVGVLAAFGWSLYALFFGGAGMPGMRMTFRLLPEPGAGADEIYLEVAAAVTVFIL
                     AGRYFEARAKKRSGAALRALLDMGAKDVAVLRDGREQRVPVDQLVVGDRFVVRPGEKI
                     ATDGQVVEGASAVDASLLTGEPVPVEVGPGDVVTGATVNAGGRLIVRATRIGADTQLA
                     QMARLVEQAQSGKAPVQRLADRVSAVFVPVVIALAVGTLGFWLGAGAGAEAAFTAAVA
                     VLIIACPCALGLATPTALLVGTGRGAQLGILIKGPQILESTRRVDTIVLDKTGTVTTG
                     RMKLVDVIPAAGTVDTELLRLAGALEDASEHPVAAAVADGARERVGELPPVESFVSTQ
                     GLGVHGMVDGRAVAAGRVSWLAQEWAQPLGTDLDQARRDAESAGRTVIAVGWDGAARG
                     LLVVADTVKPTSAQAISELRGLGLRPVLLTGDNAAAARAIAAEVGIDPGDVVAEVLPA
                     DKVAKVADLQARGAVVAMVGDGVNDAAALARADLGIAMGTGTDVAIEASDLTLVRGDL
                     RAAVDAIRLSRRTLGTIKGNLFWAFAYNVAALPLAMAGLLNPLIAGAAMAFSSVFVVS
                     NSLRLRRFRALSQTPAAANPTPRPRTVTAAAGAPPRR"
     gene            complement(11415..11633)
                     /locus_tag="PLF56_00520"
     CDS             complement(11415..11633)
                     /locus_tag="PLF56_00520"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_011757178.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_function: GO:0046872 - metal ion binding [Evidence
                     IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="heavy-metal-associated domain-containing
                     protein"
                     /protein_id="HOQ52075.1"
                     /translation="MSTTATYSVTGMTCSHCVAAVTEEVSRLDGVSAVDVDLNAGGDS
                     RVTVTSSAPLPLAAVREAVDEAGYTLAS"
     gene            complement(11837..12862)
                     /locus_tag="PLF56_00525"
     CDS             complement(11837..12862)
                     /locus_tag="PLF56_00525"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_020388681.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="hypothetical protein"
                     /protein_id="HOQ52076.1"
                     /translation="MNTALKIGGYTLGLVAVFGAAMGIGAAVTPAATSAATSSPETES
                     HTDMSATTSAPTADAVPLPGGLMVTENGYTLRLTDRTLPPGPQVPLRFQILGPDGAPV
                     TGYQTEHDKDLHLIAVRRDLSHFQHVHPVLDAEGTWSVPVDLSAAGEYRVFAGFTPTG
                     HDGGLVLGADLAVPGTYEPVPLPEPVTVVEVVDGYQVTLAGDLVPGQASELTLTVSRD
                     GVPVTDLEPYLAAYGHLVALRAGDLAYLHVHPAGAPGDGVTPAGPGITFYATAPSNGD
                     YRLFLDFRHAEVVRTAEFTVHAGEMPADATVPATDPSTTATTTVPVPQTQADTGHDQT
                     VTGSHGH"
     gene            complement(13017..13328)
                     /locus_tag="PLF56_00530"
     CDS             complement(13017..13328)
                     /locus_tag="PLF56_00530"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_012919181.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="metal-sensitive transcriptional regulator"
                     /protein_id="HOQ52077.1"
                     /translation="MTAATHEHASPHGYIHRKDDYLKRLRRIEGQARGLQKMVEDEKY
                     CIDILTQVSAMTKALQSVALGLLDEHLSHCVVQAARTGGPGADEKVREASEAIARLVR
                     S"
     gene            13605..13730
                     /locus_tag="PLF56_00535"
     CDS             13605..13730
                     /locus_tag="PLF56_00535"
                     /inference="COORDINATES: protein motif:HMM:NF014721.2"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="hypothetical protein"
                     /protein_id="HOQ52078.1"
                     /translation="MAWGMIVAKFRPDRWDIIGAIIYPIGGVSISIRPVVCQGIR"
     gene            complement(13699..14004)
                     /locus_tag="PLF56_00540"
     CDS             complement(13699..14004)
                     /locus_tag="PLF56_00540"
                     /inference="COORDINATES: protein motif:HMM:NF014035.2"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_component: GO:0016020 - membrane [Evidence IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="TSUP family transporter"
                     /protein_id="HOQ52079.1"
                     /translation="MAGTLVTSVVGALTFAALALVTPGAVTPEWVLGLVCGLGGLAGG
                     YVGARLSRQLPERALTLLLGVTAIGMAVLYVAHTWPRRPATTPTPPEVSECPGRRPA"
     gene            complement(14192..>14555)
                     /locus_tag="PLF56_00545"
     CDS             complement(14192..>14555)
                     /locus_tag="PLF56_00545"
                     /inference="COORDINATES: ab initio
                     prediction:GeneMarkS-2+"
                     /note="Derived by automated computational analysis using
                     gene prediction method: GeneMarkS-2+."
                     /codon_start=2
                     /transl_table=11
                     /product="ANTAR domain-containing protein"
                     /protein_id="HOQ52080.1"
                     /translation="VGSTPSAASICTAPPGVACRTRTRTCWSCSPNTSTRGLTDYSAA
                     QPGERDAQHLQQALRSRFAVNQAIGGLMATHGITADQARTTFRQRLAETVADAEQLAH
                     DIITEHIPTTDQTDPEAT"
BASE COUNT         2176 a         5292 c         4940 g         2147 t
ORIGIN      
        1 gtccgggggt ggttggtcga agatcaccga cagcggctgc ccgaccccga cctgcccgcg
       61 gtccgggggc ggttcgaacg acggaaagat ggttccgttc ggctgcaccg tggtgaacga
      121 cacctcggtc gtggtggaca ggccggccgg gtcggtgccg gtcgcggtga gccagtaggt
      181 gcggccgtag ccgaggggtt cgttggtcga ccagctctgc tggtccgcac cgagcccgcc
      241 gtctacggtg accccgtccg ggttggtcat cgcgacctgg cccagctcac cgccggtcac
      301 ccgcacggtc accgggtcca gtaccgacac tccggttgcc ccgtcggcca cggacacctg
      361 aatcttggcc gtgctcgccc gcggagacga cgacgccgac gccccgccgc cggtcatcga
      421 cggacccgca tcggcagatg ccggtggaac cgccgccgtc acgttcgtcg acgtgtccgc
      481 ggtacttagc gcaccggtcg ccgaacaggc gccgaccagc aggaccaccg ccgtcaccaa
      541 cccggccaga cgagcagggc cgcccaccgc gggccgcagc cttgccacca ccagcacccc
      601 ccatcctcat gacgcgacgg accaccagtc gcccacacca ggctactatc tgttgtagta
      661 gagaatgggt gaagaggccg gtcgaacgcc gcgaccgaca gtgacgaatg gagcagtgtg
      721 ccgacccccc tggccgccgc catcccgtcc ccgccgcagg gtgtctggta cgtcgggccg
      781 gtcgcgctgc gggcctacgc gatcttcatc atcatcggca tcgtcgtcgc cgtctggtgg
      841 ggtaacaagc ggttcgtcgg ccgcggtgga cgtcctggcc gggtcaccga catcgcggtg
      901 ttcgccgtgc cgttcggcat cgtcggcggc cggctgtatc acgtgatcac cgataaccag
      961 ctgtacttcg gcgagggccg caatccctgg aacgcgctgg ccatctggaa cggcgggctg
     1021 ggcatctggg gggcgatcgc cctgggcggg ttcggcgcgt ggctgggctg ccgctactac
     1081 aaggtcccgc tggccgcgtt cgccgacgcg gtcgcgcccg gcattgccgt tgcccaggcc
     1141 atcggtcgtc tgggcaacta tttcaaccag gagctgttcg gcgcgccgac gacgctgccg
     1201 tggggcctgg acgtgttcgt ccgcaccccg ggcggggtcg ccggggtcat ccccgccaac
     1261 ggcaactgcg agttcccgac cgagtacatc aaggccaccc ccgaggtgct ctgcgggatc
     1321 tatcagccga cgttcctgta cgagtcgctc tggtgtctgg gcgtggccgc gctggtggtc
     1381 tgggcggaca agcggttcat cctgggtggc gggcgggcgt tcgccctgta tgtcgccggc
     1441 tacaccctcg gccgggtgtg ggtcgagatg ctgcgcatcg acccggccaa ccacttcctc
     1501 gggctacgga tcaacgtgtt cacctcgatc ctcgtgttcc tgggcgcggt gctgttcctg
     1561 tacctgcggc gaaacgtgtc gcgcgaagac ccggcggtgg tacgcggtcc gacgcgacgc
     1621 ggcgaagccg ggcagcacgc gggcggagta tcggccgagg gccggtgagg cttctggcag
     1681 acctcgcgcg cccgggcttg cccggcctgg tgcgccttgc cgctgaccga tcgttgtccg
     1741 gctcggtcat ctccccacgc agctgacgct gagcagcatc gccagagccg ccacggtcgg
     1801 cagcaggaca acggtgaggg cagcggcgac gcagcggccg aacctgacca cggcccacgt
     1861 tccacgaccc acgatcaggc ggtccagtcg acggtcgatc gccgtgtccc ccatgcccag
     1921 acccggatgc ggcagtggca cttcggacag cacggtcaac gctgtccgga ccgcgtccac
     1981 gccgtgtcgc cggacggccg cgcgatcggc ggcggcttcc accagttcac gcaacgcggg
     2041 cggggccgcg cggaacagcg gaacccaggg aaacgcagcg gccagcgcgt ccgcagcgtc
     2101 caccatcagc tgatgacgac cacgaaggtg agcccgctcg tgcgcgagcg tcgcggacac
     2161 cgcgaccgga tccagcgacg cccacagccc gtaggaggct gcgatcagcc cccgccgacc
     2221 gccgatgctg aacgcgaccg gccgcgggtc gtccaaccac atcacccgtg catccaccgt
     2281 gtccgccaaa ccggccaggg cgaccaggaa acgaaaacga tcccgttcct gacgccgtcg
     2341 caccgcctgc cgacgagcaa ccaccgcccc tcgcaggatc aaaccgacca acgccaccac
     2401 gccgaccgcc gcgacaccct cgtcccaggc cggcaccgcg gcatgggaca gaaccccgaa
     2461 acagacggcc agcagtcccc cgagcccgcc atgatcgggc aatgcgagca gcaccacccc
     2521 ggcgaccccg gccaccagca cccccgtcac gcagcccatc caggccacga tccccacccg
     2581 cggatcccgc ttagccgcca ggaggcccac caatggtcgc cgggcggccg ttgccgcgag
     2641 gaccgccccg aggagcagcg cgagagcgat gatcatttac gtcgatcccg gcgctccaac
     2701 gccgacctca gcagttcgga ttcggcgtcc gacaccgagg tcacgaagtg caacagcgcc
     2761 gcctcccggt cgccggcgcc ctggagaatc tcccgcatcg cctcggcgga ggcccggctt
     2821 cgggtgaacg ccggccggta ttgataggca cggccccgca tcacccgctc gacgtatcct
     2881 ttgcggtgca ggttgtccaa caccgtcatc accgtcgtgt aggccagccg gcggtcggca
     2941 cccagcacgt cccgcacatc ccggaccgac atcggcccgt cgccggacca cagcacgtcc
     3001 atcaccgtgg cctcgagctc acccaaccgg cgcaccgggc cccgctcctc tccctgcggt
     3061 cagcgttcgg ccgccgcgcg cggacgccca gccggataca ccctatccgc caccccggtc
     3121 gaccggatcg ctcccgactt caccggcgcg ccctgcccgc cgccgaaatc agacttgtga
     3181 ggacctcgcc gacctgtccg ctcgccactg gacgcccacc agcaggctcg ggcggcaatc
     3241 gccagcgccg ccagcgccag gacgggttgc agcggcgccc agacggcgag cgcacccgaa
     3301 ctgcccagca cggccagcac gagcttgttg cacagcgggc atcccaccgc caacaccgtc
     3361 atcgcacccg ccgccaccag cccgccccga cctctggtcg gtctggcggc ggcacccgac
     3421 accgccgcca gaccgaccag caccgacacc gtcatccaca ccgggtaact ccaccagggc
     3481 gccggagtca tccgcccgaa aaccgaagtc ggcaccagcg ccgtcggcac cccgatcacc
     3541 agggcgatcg ctgccacggc accgatcgag gcggccaagc gcggccgcct ccacccgctg
     3601 ggcagcgccc atcgtctgcg aaccggcatc acacccacct acccggctgc cggcggcacc
     3661 cgccggcgtc agagaaccgc aatactaggt atgatagtag caagtgttgg tgtggggctt
     3721 gacattggca aggaatggaa atgtcgccga agaaacgcgt gcgcgcccgc acggagcccg
     3781 cacccggacg gaccgaccgg gccgcacagc actgggctcg gcgacgccga cgccgcatcg
     3841 ccgcgggggg cctggcgggg ctcgcgattg tcgttctggt gtcccactgg ctggagcacc
     3901 tgggcctgct gcggatcatg actccggcag cgcaggacct gttcgtcgga tatcccaccg
     3961 ctttcctctt actgatcatc gccggcatcc tgtggggtgt ctgagctccg caggtcacgt
     4021 cgtacctcgc gtccgccggc cctcatccgc ggccggcggc cgtcatccgc ccgggcggac
     4081 ggcgccgatg tatccgtccc agttcattgg cgacaccttg acgaccgtgc ccgagttgcg
     4141 cgcctcgacg atctgaccgt tgcccatcca gatcgccaca tggtggatgg tggtcgggtc
     4201 ggatgtgttg tatgcccaga acaccaggtc gcccgcctgc agctcatcgc ggccgatctt
     4261 cggttgaccc gcgtactggt atgccgaata gtgcggaagc gagacgccga cctgcgccca
     4321 ggcgttgagg gtcagcccgg agcagtcgaa accgatcttc ttgtagtcgc cgaacctgtc
     4381 cgcgaccccg ccatcgcgaa taccgagcgt ggggcctgac gcgtttccac ctccccaggc
     4441 gtatgtcgtg cccagccatc tgagggcggc gtccaccacc cgctgccccc ggtcggaggt
     4501 gcgtccgctg tcctggaccg gcgctggagc cggggcagcg gacgccggcg ggttcgccgg
     4561 cccctcgcag gcgatgccat tgtcattcgg gtccagctcg ggtcggtatc cgggttcgtc
     4621 cgtggtgatc gggccgcgcc cagcggcccg cgcagcgtca caggtcgcgt agaacggctc
     4681 cgccgtcaaa gcgacctgtg cggcacgagc ggtggtggcc tgttcctcgg cgatccgtgc
     4741 ggcctcctcg gcagctgccc gctcggccac ctgccgtgcg gcctcctcgg ccgcctgccg
     4801 ggccgcttcc tccgcggccg cccgctctgt cgcctgccgg gccgcctcct cggcggccgc
     4861 ccgctcggcc gcctgccggg ccgcctcctc cgccgccttc ctggccgctt cctcggcagc
     4921 ccttcgggcg gcctcttcct ccgcctgctt ctgggcgagc cactgctgga actgctcccg
     4981 ctgttgctgc aatccgtcca ccgcggccag cgccgccgcg tagtcgctct gctgctgttc
     5041 gagccggccc tgcagcacag ccagttccgc ctggccggcg cccagcgcct gatcggcatc
     5101 gacagcggcc tggtccgccg ttgccttcgc ctgctcggcc cgggtctggg tgatcgcggc
     5161 gttgtcccgt gctgcgccgg cctcggacgt caaattggct tgccggatgc gttgcgcgtg
     5221 cagccgatcg acggcgttga gcacgctgcc cgacatctgg tccagcagtt cgcggcgtcc
     5281 cagcaggtcg gtgggactgc cggcgtccag cagggcggtc atcgagccca gcaccgaccc
     5341 ctgccggaag gacgcggccg cgaatgccgc agcatccccc tcggccgcct cgacggcctg
     5401 cccagcggca tcgagctccg actgcgcgga cgtcgccgcg gcctgtgccg cagcggcatc
     5461 ggattgggcg acctgcagat ccacagcggc cttgttcgcc agctccatct gcagttccat
     5521 ccggtcttgg agccgctgaa tgtccccctc ggtgcgcgcc accagaccgg ccaggcggcc
     5581 cgcttcggcg gccgcggcgg tggcctgatc ccggctggcg ttgagctcgt cgtcgctcgg
     5641 gttcgggggc ggtggcggga ccgcggcggc cggtaccgaa gtaaggggcg ccagggccag
     5701 taacgccgtc acgaccaaga cccgccatcc caaccgactg acctccaatt acgacatcag
     5761 gtagtagtac catacgtcgc tctggtcact ttggcctcag atgaatcaac cgcaggagag
     5821 atatccggtt aaagccgaca aatcaagtcg ccggggacca gccaggaact ccgacattcc
     5881 gtagtagcat cccgggggtt tgagccaact ggtggcgccg ttcccgagtc ccgcgcatca
     5941 tgttcgatcc gaccccgagg aagaatcatg agcctgcccg cccagatgct cgacagtcat
     6001 ccagcggctg cgttcgcgac ggcgaaccag gccattgcgg aatgtatcca ggcctgcgtg
     6061 gagtgcgcac aggcctgcat cgcgtgtgcc gacgcctgcc tggcggagac catggtggcc
     6121 gacctggtcc gatgtatcac atccgacctg aactgcgcgg acatctgtga gagcaccggc
     6181 cgagtgctca cccgacaggc cggcgccgac cgggcaatga tcttcagaat gctgcagtcg
     6241 tgcgcggccg cctgcaaggc ctgcggtgac gaatgcgaga cccactcaca ggtccacgcg
     6301 cactgccgac tctgcgccgc ggcctgtcga cgctgcgaac aggcgcgccg ggatctgctg
     6361 gaggcactgc caatcggttg atggacggtc cagcgcccgg ttgaccgacg aggcccaacc
     6421 gggcgctgga cgcacacaca gtgcggtggc gctgaatcag gcggaggtgg gcaccggcgc
     6481 agcggcgtca atccgtcgtt ggccggacgg cggcaccggg tcgggtagcc gcagccgctt
     6541 gagcagcagg gcgttgaccg cgacgatcag gcttgatccg gacatcgaca gcgcggcgat
     6601 ttcggggcgg agcatcaacc cgaacgccgg catgaacacc ccggcggcga tgggcagggc
     6661 gatcgtgttg tagccgatgg cccagcccag gttctgccgc atcttgcgca gggttccgcg
     6721 gccgatccgc agcgcggtgg gcacgtccag cgggtccgac cgcatgagca cgatgtccgc
     6781 ggtctcgatg gcgacgtcgg tgcccgcacc gatcgcgatg ccgacgtccg cctgcgccaa
     6841 cgcgggggca tcgttcaccc cgtcgccgac catcgccacg cggcgaccgg tttgctgcag
     6901 ctcagccacc ttggcggcct tgtcgccggg gagcacctcg gcgatgacct cgtcgatgcc
     6961 caactgatcg gcgatgcgcc gagcggtggc ctggttatca ccggtgagca tgaccactcg
     7021 caccccggcc tcgtgcaacg cggccacggc cgccgacgcg gtgtcccgcg gggcgtcggc
     7081 cattccgatc accgcgacgg cctgcccgtc gaccgcggcg aacaccgccg tgcggccggt
     7141 gtcggccaac tcgtcccgcc gcgcggccaa cccacccagg tcgacatcca gttcggccat
     7201 cagcttgcgg ttgccgacga tgacccgctg cccgtcgacg tcggcaaggg cgccgtgacc
     7261 ggccactgat cggaaaccgg tggccgccag gcggggcgcc tgccgcccgt ccgcgtgacg
     7321 aaccaccgcg gcggccagcg gatgttcgga ctcccgctcg accgcggcga ccaacgccaa
     7381 cacccgcagg gtgtcctgat gatcggcggt gatgacgtcc gtgacctcgg gctcgcccct
     7441 ggtcagcgtg ccggtcttgt ccatcaccac ggtgtcgacc cgggcggcgg tctccaacgc
     7501 ggtcgcgttc ttgaacagca ccccccgctt ggcgcccaac ccggtgccga ccatgatcgc
     7561 cgtcggggtg gccaagccca gggcgtcggg gcaggtgatg accaccacgg tgatggcgaa
     7621 cagcaacgcg gtctgcacgc cggcgccggc cagcagccac acggcgaagg taagggtgcc
     7681 accgatcagc gcgaccagca ccagccagaa cgcggcccgg tcggcgagcc gctgcccggg
     7741 cgccttcgag ttctgcgctt cctggaccag ggccacgatc tgcgccaacg cggtgtccgc
     7801 gccgaccttg gtggcccgca cccgcagcga ccccgacacg ttgaccgatg cgccgatcac
     7861 ctcgctgccg ggttgcttgg gcacgggcag actctccccg gtgaccatcg actcatcgac
     7921 ctcggactcg ccgtcctcga cggcgccgtc gaccgggatc ttggcgcccg ggcgcaccag
     7981 gagcaggtca ccgacggcca cctccgacgt cggtacctcc acctcccgcc catcccggat
     8041 cacgattgcc ttcggcggag cgagctcgag cagggtgcgg atcgcgtcgt tcgcgccgcc
     8101 gcgggcccgc atctcgaacc agtgcccgag cagcacgaac gcggtcaaca cggtggcggc
     8161 ctcgtagaac acctcaccgc cgccggtcag ggtgaccccg acgctgtaca gccagccggc
     8221 ggccaccgcg accgcgacga gcaccatcat gtccagcgtc cgggcccgca gcgcccgata
     8281 ggccccgtcg aagaagatcc aggcggagta gaacaccacc ggcagcgaca ggatcagcga
     8341 aaacacgtcg tcccgcagcc cgaacggggc gggcagggtg aaacccaaca cctcccggcc
     8401 gatcggcgac cacaacagga tcggcaccga cagaaccgcg gcgaccagga accggttgcg
     8461 catgtcccgg accatcgcgt ccatcgacat cccgccaccg tgatggccgt ggccgccatg
     8521 gcccatcacc tcctgcgggc tgcgtccggg tgcgtggtca tgacccggcc cggcgtggtc
     8581 ggcggaggtg gccgacacgt gtgtcatggc agtgtgcgca ccgtgttcgg catggtccgc
     8641 cggttcggca attaccgagg gcgcgccatg gccagcgtgc gtcgcagctg cagcctggcc
     8701 gtgggcggat tggccggggg gttcggccaa cgggtcgcag atgtggtcgg gcacggattg
     8761 cccgctgcag tggtagccgc agtcacggat ccagccgcgc aggtcggcga ccgtggtgcg
     8821 ggtcgggtcg taggtcacgg tcgcggtctg ggacaccggg ttcgcgtcca ccgcgtgcac
     8881 gccgggccgg cgacccagga cggcctcggt gacggccttg gaagacgccc agtgcacacc
     8941 ggacacctcg acgacgacgg tcctgtcggc gctggtcatt gaatgttcct tttcgatcag
     9001 acggtggacg aatgactggt cagcggcgcg gaggcgcgcc ggcggccgca gtcaccgtcc
     9061 gaggacgagg ggttgggttc gccgcggcgg gggtttgcga gagtgcgcgg aaccgccgca
     9121 gccgcagact gttgctgacc acgaacaccg aggagaacgc catcgcggcg ccggcgatca
     9181 aggggttcag caggccggcc atggccagcg gcagggcggc cacgttgtag gcgaacgccc
     9241 agaacaggtt gcccttgatg gtgcccaggg tgcgtcggga cagccggatc gcgtccaccg
     9301 ccgcgcgcag gtcgccgcgg accagcgtca ggtcggaggc ctcgatggcc acatctgtcc
     9361 cggtgcccat cgcgataccc aggtcggccc gcgccagcgc ggcggcgtcg ttcaccccgt
     9421 cgccgaccat cgcgaccacc gcgccccggg cctgcaggtc ggcgaccttg gccaccttgt
     9481 ccgccggcag cacctccgcg acgacgtccc cggggtcgat gccgacctcg gcggcgatcg
     9541 cccgagcggc ggccgcgttg tccccggtca gcagcaccgg ccgcaggccc agcccgcgca
     9601 gttcggaaat ggcctgcgca ctggtcggct tcaccgtgtc ggcaaccacc agcaggcccc
     9661 gggcggcgcc gtcccagccg acggcaatca ccgtccggcc ggccgactcc gcgtcccgcc
     9721 gagcctggtc caggtcggta cccaacggct gggcccattc ctgggcgagc caggacaccc
     9781 ggcccgcggc gacggcgcgg ccgtccacca ttccgtgcac acccagaccc tgggtggaga
     9841 cgaacgattc caccggcggg agctccccga cgcgttcccg ggcgccgtcc gcgacggccg
     9901 cggcgaccgg atgttcggag gcgtcctcca gtgccccggc cagccgcagc agttcggtgt
     9961 ccactgttcc ggcggccggg atgacgtcga ccaatttcat ccggccggtg gtgacggtgc
    10021 cggtcttgtc cagcacgatg gtgtcgaccc ggcgggtgga ctcaaggatc tgcgggccct
    10081 tgatcaggat gcccagctgc gcgccgcggc cggtgcccac cagcaacgcg gtcggggtgg
    10141 ccaacccgag ggcgcagggg caggcgatga tcagcacggc cacggcggca gtgaacgccg
    10201 cctcggcgcc cgcgccggcg cccagccaga aaccgagagt gccgacggcc agggcgatca
    10261 cgacgggcac gaagaccgcg gacacccggt cggccagccg ctgcaccggg gccttgccgg
    10321 actgcgcctg ctcgaccagg cgggccatct gggccagctg ggtgtcggcc ccgatccggg
    10381 tggcccggac gatcagccgc ccgccggcgt tgacggtggc cccagtgacg acgtccccgg
    10441 gaccaacctc gaccggcacg ggttccccgg tgagcaggct cgcgtcgacc gcggacgcgc
    10501 cctcgaccac ctgaccgtcg gtggcgatct tctcccccgg ccggaccacg aaccggtccc
    10561 cgaccacgag ttggtcgacg ggcacccgtt gttcgcggcc gtcccgcagc acggccacgt
    10621 ctttcgcgcc catgtccagc agcgcccgca gcgccgcacc ggaccgcttc ttggcgcggg
    10681 cctcgaagta ccggccggcc aggatgaaca cggtgaccgc ggcggcgacc tccaggtaga
    10741 tctcgtccgc gccggccccg ggctcgggca gcagccggaa cgtcatccgc atgccgggca
    10801 tgccggcgcc cccgaagaac aaggcgtaca gcgaccagcc gaacgcggcc aggacaccga
    10861 cgctgatcaa ggtgtccatg gtcgcggcgc cgtgccgcag gttcgtccag gcggcccggt
    10921 ggaacggcca cgcgccccac acgacgaccg gcgcggcgag cgtcaggctc agccactgcc
    10981 agttgtcgaa ctgcaacggg gcgaccatcg ccagcagcag caccgggacg gtgagcgccg
    11041 tggagacgac gagccggcgg cgcagcgagg ccacctccgg gtcctcatcg gcctcggccg
    11101 gcccccgcgt cgccgcgccg gtatccggtg ggcgcggcgg tgcgggcacc gtggcggtgt
    11161 agccggtggc ctccacggtg gcgatcgccg ccgccacctc ggtgccctcc gggagcacca
    11221 cgtgcgcctt ttcggtggcg tagttcaccg aggcctgcac cccgggcatc cggttgagct
    11281 tcttctcgat ccgcgccgcg cacgacgcgc acgtcatgcc accgatctgc agatcgaccg
    11341 cgtgctccgt catgccacga acctccttgg ggatgtctat tagtgccgcg gatggccgct
    11401 gccgtgatcg cccgtcagga agccagggtg tacccggcct cgtcgacggc ctcgcgcacg
    11461 gcggccaggg gcagcggtgc gctgctggtg acggtcaccc gggagtcgcc gccggcgttc
    11521 aggtcgacgt cgacggcgct gacgccgtcc agccgggaga cctcctcggt gaccgcggcg
    11581 acgcagtgcg agcaggtcat gccggtgacg gaataggtcg ctgtggtgct catcgggtcc
    11641 tccaagacaa gtggtgatcg atcagtgatt tcgttggcgt ggggcgagtt cgggagcgtt
    11701 cccgtcagcc cggcagggcg tcgaatcggc cgcaattcag tgggtgcaca tcggacgact
    11761 cccttcgaaa ctccctgact tctttatacc cccccagggt gtacttgtaa acccccagcg
    11821 ggtatgcaac tgggggtcag tgtccgtggc tgccggtcac ggtctggtcg tgcccggtgt
    11881 cggcctgggt ctgcgggaca gggaccgtgg tggtcgcagt ggtcgacgga tcggtggccg
    11941 ggaccgttgc gtcggcgggc atttcgccgg cgtgcacggt gaactcggcg gtccggacga
    12001 cctcggcgtg ccggaagtcc aggaacagcc ggtagtcccc gttggagggc gcggtggcgt
    12061 agaaggtgat accgggaccg gccggggtga ccccgtcgcc gggggcaccc gcgggatgga
    12121 cgtgcaggta ggccaggtcc ccggcccgca acgcgaccag gtgcccgtac gcggcgaggt
    12181 agggctccag gtccgtcacc ggaacgccgt ccctgctaac cgtcagcgtc agctccgacg
    12241 cctgtcccgg caccagatcg ccggccaagg tgacctgata gccgtcgacg acctccacga
    12301 cggtgaccgg ttcgggcagc ggcaccggct cgtacgtgcc gggcacggcg agatccgcgc
    12361 cgaggacaag tccgccgtca tggccggtcg gggtgaagcc tgcgaagacc cggtactccc
    12421 ccgccgcgga caggtccacc ggcacactcc aggtgccctc ggcgtccagg acggggtgca
    12481 cgtgctggaa gtgcgacagg tcgcggcgga ccgcgatcag gtgcaggtcc ttgtcgtgct
    12541 cagtctggta tcccgtgacc ggcgcaccgt cggggcccag gatctggaac cgcagcggca
    12601 cctgcggccc gggcggcaag gtccggtcgg tcagacggag ggtgtagccg ttctcggtga
    12661 ccatcagccc gcccggcaac ggcaccgcgt cggcagtggg cgccgaggtc gtcgcggaca
    12721 tgtcggtgtg gctctccgtt tctggcgacg acgttgccgc gctggtggcg gctggcgtga
    12781 cggccgcgcc gattcccatg gcagcgccga acacggccac caggccgagg gtgtagccgc
    12841 cgatcttcag ggcggtgttc atcgcggatt cctcccgtgg tgcgctgacc cacccgaagg
    12901 gtgcggtcag ctatggacgt ggggagcgcc cgaggttgaa cgtggcgccg ttaaccgact
    12961 cgactggccg ggtgtgcggc cgcacggcac cttgtgggtc ctgcggtagt ggtcggttag
    13021 gagcggacca gccgggcgat cgcctcggaa gcctcacgga ccttctcgtc cgcccccgga
    13081 ccgccggtcc gggcggcctg caccacgcag tggctcagat gctcgtccag caggcccaac
    13141 gccaccgact gcagcgcctt ggtcatcgcc gagacctggg tcaggatgtc gatgcaatac
    13201 ttctcgtcct cgaccatctt ctgcagtccg cgggcctgac cctcgatgcg gcgcagccgc
    13261 ttcaggtagt cgtccttgcg gtggatgtat ccgtgcgggc ttgcgtgctc gtgcgtcgcg
    13321 gcggtcatcg tggcgctcca cttctctcag ggatccggct tcgaaatccc tatatcaagt
    13381 actcccgacc ggtactcgct tccctctgga atagagtacc cccctagggt acagggagca
    13441 accagcaggc cgccggtccc tgtcatcact cccttcaagg tcgagctcgc acccggtccc
    13501 ccatttctac cccctaccag taggtagtac gtcaagtcgg cgcagtcagc actcgccacc
    13561 cgcggtgatg ggcccttggg ctcatcgccg ccgcccagtt atccctggcc tgggggatga
    13621 tcgtggctaa attccggccc gaccgctggg acatcatcgg cgcgatcatc taccccatcg
    13681 gtggtgtgtc gatttcgatc aggccggtcg tctgccaggg cattcgctga cctcgggtgg
    13741 ggtcggggta gtcgcaggtc gccgtggcca tgtatgcgcc acgtacaaga cggccatgcc
    13801 gattgcggtc acaccgagca acagggtgag ggctcgctcc ggcagctgcc ggctgagtcg
    13861 agctccgaca tagccgccgg cgagcccacc aaggccgcag accaggccca gcacccattc
    13921 cggcgtgacc gctcccgggg tgaccagcgc caacgcggcg aaggtcaacg ccccgaccac
    13981 cgacgtgacc agagtgccgg ccatgcaatt atcgcgagct cgacttcgca gtcgcccagc
    14041 cgctggtcga tccggtggac ggctcctggc gcgaactgat tagaccgctc acagagagcc
    14101 cctataggag gacactcacg acccgagaga ttccgagcgt cgagctctcc tcctcgacac
    14161 ccgccgcctg ccgggggcag cgggtcgggg ttcaggtggc ctcgggatcg gtttggtcgg
    14221 tggtcgggat gtgctcggtg atgatgtcgt gggcgagttg ttcggcgtcc gcgacggttt
    14281 cggcgagtcg ttgccggaag gtggtgcggg cctggtcggc ggtgatgccg tgggtggcca
    14341 tgaggccccc gatggcctgg ttgacggcga accggctgcg cagggcctgt tgcaggtgct
    14401 gcgcgtcgcg ttcgccgggt tgggcggcgg agtagtcggt cagaccccgg gtcgaggtat
    14461 tcggtgagca cgaccagcag gtccgagtcc gggttctgca ggccaccccg ggtggcgctg
    14521 tgcagattga ggcagccgac ggggtgctgc cgacc
//
LOCUS       DAOSUG010000008        14222 bp    DNA     linear   ENV 10-JUN-2024
DEFINITION  MAG TPA_asm: Micropruina sp. isolate AMR_MDS_2195
            NODE_2567_length_14222_cov_4.283264, whole genome shotgun sequence.
ACCESSION   DAOSUG010000008 DAOSUG010000000
VERSION     DAOSUG010000008.1
DBLINK      BioProject: PRJNA925477
            BioSample: SAMN32864262
            Sequence Read Archive: SRR4236673
KEYWORDS    WGS; ENV; Metagenome Assembled Genome; MAG; Third Party Data; TPA;
            TPA:assembly.
SOURCE      Micropruina sp. (wastewater metagenome)
  ORGANISM  Micropruina sp.
            Bacteria; Bacillati; Actinomycetota; Actinomycetes;
            Propionibacteriales; Nocardioidaceae; Micropruina.
REFERENCE   1  (bases 1 to 14222)
  AUTHORS   Abdulkadir,N., Saraiva,J.P., Zhang,J., Stolte,S., Gillor,O.,
            Harms,H. and Rocha,U.
  TITLE     Genome-centric analyses of 165 metagenomes show that mobile genetic
            elements are crucial for the transmission of antimicrobial
            resistance genes to pathogens in activated sludge and wastewater
  JOURNAL   Microbiol Spectr 12 (3), e0291823 (2024)
   PUBMED   38289113
REFERENCE   2  (bases 1 to 14222)
  AUTHORS   Abdulkadir,N.
  TITLE     Direct Submission
  JOURNAL   Submitted (24-MAR-2023) Environmental Microbiology, Helmholtz
            Center for Environmental Research -UFZ, Permoserstr 15, Leipzig
            04318, Germany
COMMENT     The annotation was added by the NCBI Prokaryotic Genome Annotation
            Pipeline (PGAP). Information about PGAP can be found here:
            https://www.ncbi.nlm.nih.gov/genome/annotation_prok/
            
            ##Genome-Assembly-Data-START##
            Assembly Method        :: SPAdes v. 3.11.1
            Genome Representation  :: Full
            Expected Final Version :: Yes
            Genome Coverage        :: Not Applicable
            Sequencing Technology  :: Illumina MiSeq
            ##Genome-Assembly-Data-END##
            
            ##Genome-Annotation-Data-START##
            Annotation Provider               :: NCBI
            Annotation Date                   :: 05/04/2023 04:57:10
            Annotation Pipeline               :: NCBI Prokaryotic Genome
                                                 Annotation Pipeline (PGAP)
            Annotation Method                 :: Best-placed reference protein
                                                 set; GeneMarkS-2+
            Annotation Software revision      :: 6.5
            Features Annotated                :: Gene; CDS; rRNA; tRNA; ncRNA
            Genes (total)                     :: 2,776
            CDSs (total)                      :: 2,750
            Genes (coding)                    :: 2,714
            CDSs (with protein)               :: 2,714
            Genes (RNA)                       :: 26
            tRNAs                             :: 24
            ncRNAs                            :: 2
            Pseudo Genes (total)              :: 36
            CDSs (without protein)            :: 36
            Pseudo Genes (ambiguous residues) :: 0 of 36
            Pseudo Genes (frameshifted)       :: 2 of 36
            Pseudo Genes (incomplete)         :: 32 of 36
            Pseudo Genes (internal stop)      :: 2 of 36
            ##Genome-Annotation-Data-END##
FEATURES             Location/Qualifiers
     source          1..14222
                     /organism="Micropruina sp."
                     /mol_type="genomic DNA"
                     /submitter_seqid="NODE_2567_length_14222_cov_4.283264"
                     /isolate="AMR_MDS_2195"
                     /isolation_source="Activated sludge of municipal
                     wastewater treatment plants"
                     /db_xref="taxon:2737536"
                     /environmental_sample
                     /geo_loc_name="Canada"
                     /lat_lon="49.25 N 123.1 W"
                     /collection_date="2015-06-16"
                     /metagenome_source="wastewater metagenome"
                     /note="metagenomic"
     gene            complement(<1..708)
                     /locus_tag="PLF56_00550"
     CDS             complement(<1..708)
                     /locus_tag="PLF56_00550"
                     /inference="COORDINATES: protein motif:HMM:NF015649.2"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="BTAD domain-containing putative transcriptional
                     regulator"
                     /protein_id="HOQ52081.1"
                     /translation="MAAVDFRVLGRVGVRLDGLPQRLRPMEATVLAVLLAEANRPLSA
                     DALIDRVWRGEPPRTAATALRVHVDRLRAALGPQQATRLVTAAGGYRLIVQPDELDAT
                     RFESALRQARESAAGDPASAADVLRAALREWQGTPFDSIEGIESITMTRSYLERRRAE
                     LLTELAEVELAAGRHAAAVADLRRWCAEFPESEALASSLVIALYRSGDPIAALEECRA
                     FVRRFSDEYGLDATRAFR"
     gene            complement(1122..2381)
                     /locus_tag="PLF56_00555"
     CDS             complement(1122..2381)
                     /locus_tag="PLF56_00555"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_015069887.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_function: GO:0022857 - transmembrane transporter
                     activity [Evidence IEA];
                     GO_process: GO:0055085 - transmembrane transport [Evidence
                     IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="MFS transporter"
                     /protein_id="HOQ52082.1"
                     /translation="MSETDSESVPLEPLHTFASLSVHNYRLLFLGSLTSNVGTWMARV
                     AQDWLVLTQLTDNSASALGYVTALQFAPGLLVSPLAGAAADRFGKRRIMMVAQAGTGL
                     VFAALTALVATGAVQLWHVYLSAAIAGVLWGFDAPARQAFVSEVVPDALVSNAVGLNS
                     ASFNGARLLGPAIAGLMIAAWGIWPVFAINTLSFVAVLLGLYLMNPDELRPAPVRRGK
                     GAIREGLAYVRARPDLQLILFCMFMLGTFGLNFQITNALMATREFGVGPEGYGLLGSV
                     MAAGTLAAALLAARRRQPRMRLLVGALAAFAVSMTLLAMSPSYWVYAVLLVPVGLTSL
                     TVMTAANTTVQLTTEPAMRGRVMSLYMAIFTGGTPLGAPLIGWVGDAWGPRWTLLVGA
                     IATGAAALLAGAYLWNRMGRPTHRVDF"
     gene            2550..3206
                     /locus_tag="PLF56_00560"
     CDS             2550..3206
                     /locus_tag="PLF56_00560"
                     /inference="COORDINATES: protein motif:HMM:NF033545.0"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_function: GO:0004803 - transposase activity [Evidence
                     IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="IS630 family transposase"
                     /protein_id="HOQ52083.1"
                     /translation="MPRFEEKLIDVVGLYLNPPARAVVFSYDEKTQCQALDRTQPSLP
                     MKPGRAGTMTHDYKCNGTIDLFAAMNLATGQVLTGLNKGHTGSDVLRFFKQIDATVPR
                     GLGVHVVLDNLSAHSTPEINKWLAPRDRRRWHLHFTPTSSSWLNLIERWFKELTDRRL
                     RRGTFTSVDELSQAITTWAEHWNSDPKPFIWKAPADDIITKVQRGRDTLHQINSQTDH
                     "
     gene            3965..4570
                     /locus_tag="PLF56_00565"
     CDS             3965..4570
                     /locus_tag="PLF56_00565"
                     /inference="COORDINATES: protein motif:HMM:NF012235.2"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_function: GO:0005524 - ATP binding [Evidence IEA];
                     GO_function: GO:0016887 - ATP hydrolysis activity
                     [Evidence IEA];
                     GO_function: GO:0042626 - ATPase-coupled transmembrane
                     transporter activity [Evidence IEA];
                     GO_function: GO:0140359 - ABC-type transporter activity
                     [Evidence IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="ABC transporter ATP-binding protein"
                     /protein_id="HOQ52084.1"
                     /translation="MSINVHPGEFVVVKGSSGSGKTTLINVIAGIITPDSGSATVLDC
                     EVTGLSGAKRADLRLKRLGVVFQDDNLLADLTAVENVVLPMIVQGLASQEAKAAAQQQ
                     LSRLGLNALADRYPVEMSGGQRQRVGIARALVGGKPLLLADEPTGSLDSVNGLALFGL
                     IADLCEDGIGALVASHDSDILTFAHRVYEMKDGRVVDERSL"
     gene            complement(7746..8252)
                     /locus_tag="PLF56_00570"
     CDS             complement(7746..8252)
                     /locus_tag="PLF56_00570"
                     /inference="COORDINATES: ab initio
                     prediction:GeneMarkS-2+"
                     /note="Derived by automated computational analysis using
                     gene prediction method: GeneMarkS-2+."
                     /codon_start=1
                     /transl_table=11
                     /product="hypothetical protein"
                     /protein_id="HOQ52085.1"
                     /translation="MINASDYDSWCETAPVCHRKVSNYVEEVKGNAAYGNQNGVIGTF
                     DIVIRSNLNGRQVQWRVAYIWDSGPSIKFEYPQISCWDRNPIGVWYGCGSYVVTNGST
                     APKVSSGSWRWDSRRLDSARLEQDDTYHGAHDTYFAPKRIPPLCGRNSQHRGLQLFLK
                     RHVQVLVG"
     gene            complement(8685..8876)
                     /locus_tag="PLF56_00575"
     CDS             complement(8685..8876)
                     /locus_tag="PLF56_00575"
                     /inference="COORDINATES: ab initio
                     prediction:GeneMarkS-2+"
                     /note="Derived by automated computational analysis using
                     gene prediction method: GeneMarkS-2+."
                     /codon_start=1
                     /transl_table=11
                     /product="hypothetical protein"
                     /protein_id="HOQ52086.1"
                     /translation="MTNKAGGECGSLADRASRTRNGGGNPNNANAHPRPQQAHAKPAL
                     PAHVTVTVIGTRCPGILLE"
     gene            9742..10212
                     /locus_tag="PLF56_00580"
     CDS             9742..10212
                     /locus_tag="PLF56_00580"
                     /inference="COORDINATES: protein motif:HMM:NF024854.2"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_function: GO:0003700 - DNA-binding transcription factor
                     activity [Evidence IEA];
                     GO_process: GO:0006355 - regulation of DNA-templated
                     transcription [Evidence IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="MarR family transcriptional regulator"
                     /protein_id="HOQ52087.1"
                     /translation="MSELTDRLNMAIRTVGFASRYAADEWVRSSGLTRQQAFTLGYIE
                     EHQHRGVIARELAEMNGTTPASVASLLQGLEERGYLVRTPSPKDSRVKLLSITPQAAG
                     LIDGFDDAMRASQERLFSPLNHDEQVQLLALLERLTAEVPDAPSPPDRRRNSPT"
     gene            10373..11797
                     /locus_tag="PLF56_00585"
     CDS             10373..11797
                     /locus_tag="PLF56_00585"
                     /inference="COORDINATES: protein motif:HMM:NF013703.2"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_component: GO:0016020 - membrane [Evidence IEA];
                     GO_function: GO:0015297 - antiporter activity [Evidence
                     IEA];
                     GO_function: GO:0042910 - xenobiotic transmembrane
                     transporter activity [Evidence IEA];
                     GO_process: GO:0055085 - transmembrane transport [Evidence
                     IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="MATE family efflux transporter"
                     /protein_id="HOQ52088.1"
                     /translation="MPESNRTTLAVAPIWRALLHLCIPMIAGFSVGAVYNIINSGFIG
                     SLHSTPLLAALTFSLPVFALIMAIGGVFGVGGGTYISRLLGSLEDAETDPIATATRIR
                     QVSSFTLWGALAGGAVIGLLGLVFATPIAVAVGASGESLAPTAVYIGAMFAFTPLYVA
                     CFALEQVVRAEGAAVASMTGVIASTIANLAFDVLFILVLGWGVLGAGIALGLSNLVMV
                     GYYVWWLVRRSSVAALGPRWFRADREMLRTVFGVGVSELLQSSFLIVTTLVMNWIAIG
                     YGDALLASMGVALRISQLPEMICMGVFMGAVPLFAYAFGAGNHARLHRAIGGAAVGIL
                     GFTLVFSGLVFAFRDQVFGLFSADPAVLSDGTAILTAMLVATLFNGITGLLIAVFQAT
                     EQMRNATIMSIAQGVLFIPVVLLGNAWFHLDGVIWSMTVTELLTFALGGLLFLTSRRA
                     LDAAPSAESSAAAAELVDGAVATA"
     gene            11831..12265
                     /locus_tag="PLF56_00590"
     CDS             11831..12265
                     /locus_tag="PLF56_00590"
                     /inference="COORDINATES: protein motif:HMM:NF033788.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_function: GO:0003677 - DNA binding [Evidence IEA];
                     GO_function: GO:0003700 - DNA-binding transcription factor
                     activity [Evidence IEA];
                     GO_process: GO:0006355 - regulation of DNA-templated
                     transcription [Evidence IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="metalloregulator ArsR/SmtB family transcription
                     factor"
                     /protein_id="HOQ52089.1"
                     /translation="MIETDEDRADALFHALADRTRRDILRRVLAGEHSVSTLAKHYEM
                     SFAAVQKHVAVLERAGLLTKRRQGREALASGDVQVVRAANQLLSELESVWRGRIARID
                     ALLVENSQHDNAHSQGADSRPSIPPPVPRHQHKPTPVDRSEE"
     gene            12272..12760
                     /locus_tag="PLF56_00595"
     CDS             12272..12760
                     /locus_tag="PLF56_00595"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_003884123.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="SRPBCC domain-containing protein"
                     /protein_id="HOQ52090.1"
                     /translation="MPVVDVIKNLDGPSLTIVADFAAPVERVWQLYADPRQLEQIWGP
                     PEYPATVVQHDLTPGGRVTYFMTGAGGDRHFGYWDVAEVTAPEHFSFADGFANDEFEP
                     NTNLPVSHNSYTFGAHQGGTRATYVSTFASANDLQQVLDMGVEEGTIAATNQIDRLLA
                     LD"
     gene            12849..13277
                     /locus_tag="PLF56_00600"
     CDS             12849..13277
                     /locus_tag="PLF56_00600"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_013864560.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_function: GO:0004601 - peroxidase activity [Evidence
                     IEA];
                     GO_process: GO:0006979 - response to oxidative stress
                     [Evidence IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="OsmC family peroxiredoxin"
                     /protein_id="HOQ52091.1"
                     /translation="MATQSKAQAHWEGSLMEGAGSVDLVTSGVGHFDLKWAKRAEASA
                     GATNPEELIAAAHASCYSMALSHALAGNGTPAESIDTTAVVGFQPGVGITGSDLTVKA
                     KVPGLTENEFQKFADEAKTGCPVSAALTGIPITLTAEFIS"
     gene            13610..>14222
                     /locus_tag="PLF56_00605"
     CDS             13610..>14222
                     /locus_tag="PLF56_00605"
                     /inference="COORDINATES: protein motif:HMM:NF023568.2"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="alpha/beta fold hydrolase"
                     /protein_id="HOQ52092.1"
                     /translation="MTIVPGAEPISVDGSDVGVLLCHGYPSTPQSMRPWAEHLAAAGY
                     TVRVPLLPGMGTHWRDLNATGWQDWYGEIDRAHAELSARCSTVFAFGLSMGGLLATKL
                     AQEKPDLAGLVLVNPIFAHNNPALKILPLLRHVVPALGGLAGDIKKPGVIELAYDRNP
                     LQAMHSQTKLWRIVVEDLPQLRLPVLLITSSEDHVVPPLSWQTF"
BASE COUNT         2603 a         4696 c         4406 g         2517 t
ORIGIN      
        1 ccggaaggca cgggtggcgt ccaggccgta ctcgtcggag aagcgccgca cgaacgcccg
       61 gcactcctcc agcgccgcga tcgggtcgcc gctgcggtac agcgcgatca ccagactgga
      121 cgcgagcgcc tcggattccg gaaactcggc gcaccagcgg cgcaggtcgg cgaccgccgc
      181 ggcgtgccgg ccggcagcga gttcgacctc ggcgagctcg gtgagcaact cggcccggcg
      241 ccgctccagg taactgcggg tcatggtgat cgactcgata ccctcgatcg agtcgaaagg
      301 ggtgccctgc cactcacgca gtgcggcacg cagcacgtcc gcggcggacg ccgggtcgcc
      361 cgcggcgctc tcgcgggcct gacgcaacgc cgattcgaac cgggtggcgt cgagttcgtc
      421 cggctgcaca atgagccgat atccgccggc ggcggtgacc aggcgggtgg cctgctgggg
      481 tccaagggcc gctcgcagcc ggtcgacgtg cacccgcagc gcggttgccg cagtccgggg
      541 cggttcacca cgccacaccc gatcgatcag ggcatcggcg ctgagcggac ggttggcctc
      601 ggccagtagc acggccagca cggtggcctc catcggacgc agccgctgcg gcagcccgtc
      661 caacctcacc cccacccgtc ccagcacgcg gaaatcgacc gctgccatgg ggcctccggg
      721 ggttgtgaga gtgtgcacag cacactaacc actccggccc ggcttgggca gcacgccctg
      781 cgatgtgtga cacgacgtac tcctgcctga ggtctgttgc caggatccgt ccgcgccgta
      841 aacgtgcgat aaaacgaagc cgccgaagct caccgcgcaa ggcccacccg gcaaggcaca
      901 cacccggcag cccaccaggc aaggcccacc cggcaaggcc aagcccacga gggaacctca
      961 gcccaggtcc ggtcccacga tgcccttcgc gcagaccggg gagccggtcc gcgcggcaac
     1021 gggcggcagg ctccggtgga ccgtgtacag cgggtgggtt ttgccaccgc agtcggtgct
     1081 gaaacccacc acttcgcgaa ctgagcctcg ggccaccggg ctcagaagtc gacgcggtgg
     1141 gtcggacggc ccatccgatt ccaaaggtag gccccagcca gcagggccgc ggctccggtg
     1201 gcgatggcgc ccaccagcaa cgtccagcgg gggccccagg catcgccgac ccagccgatc
     1261 agcggggctc ccagcggtgt gccgccggtg aagatcgcca tgtagagact catcacgcga
     1321 ccgcgcatgg cgggctcggt ggtcagttgc accgtggtgt tggccgcggt catcacggtc
     1381 agcgaggtca gtccgaccgg caccagcagc acggcataga cccagtagct gggcgacatc
     1441 gccagcagcg tcatgctcac cgcgaacgcg gccagcgcgc ccaccagcaa ccgcattctc
     1501 ggttgtcgcc ggcgcgccgc cagcagcgcc gccgccagag tgccggccgc catcaccgaa
     1561 ccgagcaggc cgtaaccctc ggggcccacg ccgaactccc gggtcgccat caacgcgttc
     1621 gtgatctgaa agttcagccc gaatgtgccc agcatgaaca tgcagaacag gatcaactgc
     1681 aggtccggac gagcccgcac gtacgccagg ccctcacgga tcgctccctt gccgcgccgg
     1741 acgggggccg gcctcaactc gtccggattc atcaagtaga gcccgagcag aacggccacg
     1801 aagctgaggg tgttgatcgc gaacaccggc cagatacccc aggcggcgat catcaacccg
     1861 gcgatcgccg ggccgagcag acgcgctccg ttgaacgacg cgctgttcag cccgaccgcg
     1921 ttgctgacca gggcgtccgg caccacctca ctgacgaacg cctgccgggc cggcgcgtcg
     1981 aaaccccaca gcacaccggc gatcgcggcg ctcaggtaca cgtgccacaa ctgcaccgca
     2041 ccggtggcca ccagtgcggt gagtgccgcg aacaccagac cggtgcccgc ctgggcgacc
     2101 atcatgatgc ggcgtttacc gaaccggtcc gccgcggctc cggccagggg tgacaccagc
     2161 aggccggggg cgaattgcag ggcggtgacg tagccgagcg cagaggccga gttgtcggtc
     2221 agttgcgtga gcaccagcca gtcctgagcc acccgagcca tccaggtacc gacgttcgac
     2281 gtcaacgagc cgagaaacag cagccggtag ttgtgcacgc tcaacgaggc gaacgtgtgc
     2341 agtggctcaa gcggtacgga ttcgctgtct gtctcgctca tcgctgccgt acccacacca
     2401 tcgcaaccat cctagaacgg tgtcctgttc cctgagcccc gatccatcga tggctgcccg
     2461 ggtcgggatc ggtaaggacg cggtcgcaaa gatctgggcc gaccacaacc tcaagccctg
     2521 gcaggtgagc accttcaaga tcagcaacga tcccccgctt cgaggagaag ctgatcgacg
     2581 ttgtcgggct ctacctgaac ccgccggccc gggcggtggt gttctcctac gacgagaaga
     2641 cccaatgcca agcactggac cgtacccaac catcgctacc gatgaaacct ggccgggccg
     2701 gcaccatgac ccacgactac aaatgcaacg gcaccatcga cctattcgcg gcaatgaacc
     2761 tcgccaccgg tcaggtcctc accggtctca acaagggcca caccggttcc gacgtcctgc
     2821 ggttcttcaa acagatcgac gccaccgtgc cccgcggcct tggcgtgcac gtcgtcctgg
     2881 acaacctgtc ggcgcactcc actcccgaga tcaacaaatg gctcgccccc cgagaccggc
     2941 ggcgatggca cctgcacttc acacccacat ccagctcctg gctgaacctg atcgaacgat
     3001 ggttcaaaga actcaccgac cgacgcctcc gccgcggcac cttcaccagt gtcgacgaac
     3061 tctcccaagc catcaccacc tgggccgaac actggaacag cgaccccaaa ccgttcatct
     3121 ggaaagcccc agccgacgac atcatcacca aagtccaacg cggacgcgac accctccacc
     3181 agatcaattc acagacggac cactagaacg cctagcgggc gccacatggg gcgggccgag
     3241 tgcactcgcg cggcggtctt gcgtaggcgg acgccgtcgc gaggatcgct cgccgggtca
     3301 ggcggatagc gtcctggcgc ggaacgcgtg caggcacagg gtcagccctc gcaggcggtg
     3361 gcataggcca gcaagacgcc gccgacacgc agggcaacac cccccagtca gtgcctgcag
     3421 acggtggaat aggatacgac gccgtagagc gtcaggaacc ggcccgcgac ctaactacaa
     3481 gcaaccccac cacaaagtgg tgccccccga tcgttgtccg aatttcctca catgcgccgc
     3541 gtcgcagagg aagatcttga ccacccaaga agtcgagcct gattagtttc gtgggaaggt
     3601 ggctggccct gcgacgatcg tccgtctcgc agaatggctc gctgactcgg cattggccaa
     3661 tgtttgcgct actcgtgtta gcatacacac tccgatggaa ggaagtctgg gccatcactc
     3721 ggccagaagt tggccaagtt tcgtctgggc gcccctcacc cccggaagga cagtttatga
     3781 acaagcgcct cgcgacggtg ctagtggcgg ccggcctgat cgcaagcagt gaactcaacg
     3841 ttgcgtggtc ggacaatgat aaggccagct cctggcggac ggatcgcggc tgcgcgtgat
     3901 tctctgtgac aaactctgca agagcaatac gactcgcgtg gagactgtcc atgcactcgc
     3961 tggggtgtcg atcaatgtgc atcccggcga gttcgtggtt gtcaagggaa gcagcggttc
     4021 cggcaagaca actctgatca atgtaattgc agggatcatc actccagatt ctggctcggc
     4081 aaccgtcttg gattgcgaag tcacgggcct ctcaggcgct aaacgcgccg acctgaggct
     4141 caaacgcctc ggcgtggtgt tccaagacga caacctcctt gcggatttaa ctgcagttga
     4201 gaatgtggtt ctgcccatga ttgttcaggg actagcttca caggaggcca aagcagctgc
     4261 acagcaacaa ctttctcgat tgggtcttaa cgccttggcc gatcgctatc cggttgaaat
     4321 gtccgggggt cagcgacagc gagtggggat cgcgagggcc ctcgtcggtg gcaagccgtt
     4381 gctcctggcc gacgaaccca ccggctcact cgatagcgtg aacggtctcg cgctatttgg
     4441 gctgatcgct gatctctgtg aggatggaat aggcgcactc gtggcgtcac acgattcaga
     4501 tatcctcacg ttcgcgcatc gcgtctatga gatgaaggac ggacgggtcg ttgacgaaag
     4561 aagcctctga ggtgttgcgt ctcgtaggcg ctttacggcc gttgaagcct gcgaaacgcg
     4621 taggaatcgc ttcactactg gtcgccgggc ttctttcgct ggcacttggt atggtcaccg
     4681 cattcagcca agctcctgaa caggtggctg agcgagaact tgggatcttc gattatgtca
     4741 cgacctaccc ggcattcttc tcaatcggaa agcatgtgcc aatcgatgaa atcgaaggcg
     4801 ccctggcggc cgccaaacca acgtctgtcc ttaccgtgac aaacgtgttt ggccttgact
     4861 tccgagaatt gcccgaacag cggcgtttgt tgcttcagga ggccgattgg gcccacgcaa
     4921 tggccgaact ttcgcagtac acaataactt cgggacgact tcccgagagg cccggcgaag
     4981 tgctggtcag ccaacgattg caaggcccca acgagttgag cgcattgggt ggccgttcca
     5041 ctttcaaagt cgttggcacg tttctcaacg attatcagcg cgcttctact gtgatcctgg
     5101 ctgcgccggg gacctggagc cagatcggac gggtcacagg agcggcagcg aggtatccgt
     5161 cactccaatc atctgttcag gtgttctggc ccggcgccga tgcagataat gtggctaagc
     5221 ggttacagac cttgtcgggt atacgggccg acgaggctgg cacattgtcg cgagcagagc
     5281 tccttactga ccctatcaat attcgattaa cgtggttccc cctcatcctc gctattccga
     5341 tctccctcat acctctgctg actgggagtg ttggcgggta cctgacggga cgttggtgtg
     5401 tcggggtcga cgcgcagctc aaccgtcttg gcgttccttt gcggtctgtg cggcgcgcgt
     5461 cccgcgtgct gatttctata cgtgctgcta gttgggcgag cgcgggttgg attataggtc
     5521 tatcacttgc atggacagtt cgtccgttag tcaacctgat cgcggatcat caattgggcc
     5581 ccctaacctt attcttatcg gagtacgtag tcgtcacagg tggtctggtc gtggcgacgc
     5641 tttttggcca tttgctcgga actgggccgg tatggtcaat gccggtagga gcggcgcgag
     5701 cggcccgtgt ggcgtccatc ttgcgcccat acgttgctct tgcttccctg atagtgttcg
     5761 tcgtcactgg tattggacag caggacccac gatcggcgag cacgatggca gccgcggcaa
     5821 ttttggcagt tgtcgtcgga tcgccacccg tgagtagacg cttgctgatg gcgtttccca
     5881 ttgacactat gcggagtctg cttgccatcc gaatgcttcg ttcccagttc gggtcgggtc
     5941 gaagcgacgt gcgcagtgct ggcggccatc ttggcgatgt cgcttaccgc aacttccatt
     6001 gcgtatgcgt tggacgagac gaatcgccgc cgcacattcg atcgcgtgcc tcccgacact
     6061 gttcagatag tctcctacgc tccagagcag ccggtgccaa gccaagtgcg gattgaagtg
     6121 gaagatgcac tgggaatggc gggccccgtg aggagcgcgt cactagctcg cgacaacctc
     6181 gaagtgatga gcaacctaaa cggcgatctc cgtgtgtttg acagcgccgc agatgtgcag
     6241 cggtggaacg gggggaagct tctgacctcc gatcagagga gaactctgga cagtggaggg
     6301 gtgctcacat tcctatcgca tgatgtaatc agcattagga acggcacgct aaaggtgacc
     6361 ctacccgcgc gccaaatcga ggtgttaccc gagtggggca cgcaaggggg tggactgatg
     6421 ttggcccgca cagttcggga ccacgcgtgg aacacagaca acgaaactct gacttatagc
     6481 ggcctgtcga cttcgcaggt ctctcgggct gctgggattg cgagcgcact aggaatcgat
     6541 cctgcttacg ttgtatatcc acaccgtgaa gatgtgagca ctctggctga ccgctaccaa
     6601 gtggcattgc tagtcctggc tgtcttatgt ggtctgcttg tggccgcatt cactggcgcc
     6661 caagcccgca ccaggcgacc gcacatggca agtatgtgga cactaggagt accgactcgt
     6721 tggtttgccg tggttttggg aatccaggct gcagtcatcg tcacagtcac catcctgttg
     6781 gcggcacttg ccaccacgct taccatggtt gccgtgttca ctttcggacg cgccaacttt
     6841 gatgtagtca ttccttggcc agcactgggc ataatcggtg gagtggctgt tttcggtacc
     6901 ttgctgggac tcgccgttgg cgcacttcgg cttagatcaa gtgaaagatt tacgtgaatg
     6961 tcggactcag aggggtactt tcgggctcca tgtggcgagt ttaggcttag cgggaagcaa
     7021 tccaccacgg aaggtaccgc ttggttcgct ccacgatggc tttggtttcg gataattggg
     7081 cttgtccgct gcactagccg gcatccaagt atccccggtc aagcaaggtc acctcatcgg
     7141 ccagatttcc gcggcggctg atcccggccc agttgttcat caactggcgc ggcagtgccc
     7201 gcagctgatc atgagaggca aaaccacgga agccgggtcg gtatgacaac caacaaaacc
     7261 ggaaaataca gtctgattaa ctcatcctgg gctcgttccg ccgcgaccac ccagagccgc
     7321 ggccgcagcc gcagccatgc gcaccccgca agatggtcgt aactaagacc accgatttct
     7381 cgtggacctc atcgcgccga tcatcatcgc gggtgtcacg ggaacgtcag aggggagtgc
     7441 ctcgtgtgcg aggtaggagc ttacttcaaa attgccctgg tagtacataa tccacccggg
     7501 ccaaatgggc gccggaacat cggcttccac gagcatctcc aggatgtttt ccagggccca
     7561 ttcgtcaggg tcaccgaaca caggttcgcc ggacggttga atcaacacct cgccgggacc
     7621 ggcgacccac gcgaacatca ccccccaagc agtctgaggt aatgcagtcc aaccttgcgg
     7681 tgtgtctctc gtcgtctcga agtcgggcca ttgcatagtc gatgtaccaa cccctcagtg
     7741 gctgatcagc caaccaaaac ttgcacgtgc cgtttgagaa acaattgaag tccccggtgt
     7801 tgagaattgc ggccgcatag cgggggtatc cgcttggggg cgaaatatgt atcatgagca
     7861 ccgtgatagg tgtcgtcctg ctccagacgt gccgaatcca gccgacgact gtcccagcgc
     7921 cacgaaccgg aggagacttt gggcgcggtg ctcccgtttg tcacgacgta gctgccgcag
     7981 ccgtaccaga cgccgatggg gttccgatcc caacacgata tttgcggata ttcgaacttg
     8041 atcgatgggc cgctgtccca gatatacgca actcgccact ggacttgccg accgttaagg
     8101 ttcgatcgga tgacgatgtc aaaggtgccg atcacgccgt tttgattgcc gtaggcagcg
     8161 ttacccttca cctcttcgac gtagttgctg actttgcggt gacagacagg cgcggtctca
     8221 caccaagagt catagtccga agcgttgatc acagcacccc ttgacgaagt tacgaggcgt
     8281 cgttgagtaa ttgcggtgta gtgctgctgt gtcttgcccg acgagtcctt cgactcgtat
     8341 gtgacaccct ttcccgtgca cacccaatgg tcgagggtgt gctgaaccgc atagtcgaca
     8401 cagtcttgga gagacggttc aaccccactc gagggtgatc cagaaccctc cgcattggcg
     8461 gcctggacgg tgagtgatgg cgggacagcc aacgagacca gcgcgatgag tgctgtggcc
     8521 aaagcccgta gtcggatcac tgcggctccc agggctgagg gctgtgcgcc cggtgcccta
     8581 tgagttttcc acagctagac agcacatggc aatgtgcacc gcagcctgct tgctgcccac
     8641 accacgcgcg ccagccacat gctctgcaca gcatcatcgc gcaactactc gagcaggatg
     8701 ccgggacacc gagtgccgat cacggtgacg gtgacgtgtg ccggcagcgc aggctttgcg
     8761 tgagcttgtt gcggtctcgg atgcgcattg gcgttgttgg ggttgccgcc accgttccgc
     8821 gtgcggctgg cccgatcggc gaggctgccg cactcgccgc cggccttgtt ggtcatgccg
     8881 cgaacgggac cggccggtac cgacacgcag ctggccccgg cgaaatggga caacggcagc
     8941 gccggcccat cgagcaggca ccatgcaaga tgtgcacaga ggaccagtcg ccgaccacag
     9001 ctctactggt cgaggtgcct gtccccacta tgttggtggc cgagcaggcc ggtgcacgcc
     9061 ccacccgccg cggctggtcc accatcgcag cagttggcta cgcacgagtg cgcctcgatc
     9121 cggccgacgt caggaccgcc gtgaaggagc tgcacgctct ggacgtcgac tcggcgaaca
     9181 tccacttgga ccgcgggatg gcccggcaac gcgacacccc aactctgcag gaaagtcatc
     9241 gcaagtctcc agcagggcga cagcctggta atggcgaacc tcgcccgatt ggcgcgcagc
     9301 ttggccggac tgacagctgt ggttgggcag ctcgaacaac tgcgggttcg gctcatcgtg
     9361 gcagttactt gccaggaggg caggtacgtt gaggcagggg gcaccatcga caccatctcg
     9421 gcggcaacgt cgagaacagc ggtaaggagg ggctcccccc ggatcccctc ctgaccgtcg
     9481 cggcctcccg agcggttcgc cggttcccgt tggcggcccc cctcaccgcc accggttccg
     9541 ctaccgggat tgaaagaagt ctctgcgagc gccgtaaacg cgccgtaaac ggtgtgcgga
     9601 acgctcgttc gccagtgcgg aacgcttgtt cgccagtgcg gaacgctcgt tggccggccg
     9661 aggcggcggt gatccgggcc ttgatcagcg gacgtccaga cctccttgac cattaggttt
     9721 ctaactatta gggttctaac tatgagcgag ttgaccgacc ggctgaacat ggccatccgc
     9781 accgtggggt tcgcgtcccg gtacgcggcg gacgaatggg tgcgctccag tgggctcacc
     9841 cggcagcagg ccttcacgct cggctacatc gaagaacacc agcaccgcgg agtgatcgcc
     9901 cgcgagctgg ccgaaatgaa cggcaccaca ccggcctcgg tcgccagcct gctccagggg
     9961 ttggaagagc gcggctacct ggtccgcaca ccctcaccga aagactcccg ggtaaagctg
    10021 ctcagcatca ccccgcaggc cgccggcctg atcgatggct tcgacgatgc catgcgggcc
    10081 tcccaagaac ggctcttctc tcccctgaac cacgacgagc aggttcaact gctcgccctg
    10141 ctcgagcggc tgaccgccga ggtgccggac gctccgtcac cccccgaccg ccgccgcaac
    10201 tcacccacct gacccagagg gttcgacagg ctcgaacgac gaccggctcg accgagtagg
    10261 cgctcgaccg tccggtctcg accgagtaga cgctcgaccg tccggtctcg acccgacacg
    10321 ctcgaccagc gacaccaccc atttccaccc accgtccaac ggaggcttcg ccatgcccga
    10381 atcaaaccgc accactcttg ccgtcgcgcc gatctggcgc gccctgctgc acctctgcat
    10441 tccgatgatc gccggcttct cggtcggtgc ggtctacaac atcatcaact ccggcttcat
    10501 cggctcactg cactccacgc cgctgctggc cgcgctcacc ttctcgctgc ccgtcttcgc
    10561 cctgatcatg gcgatcggcg gcgtcttcgg cgtgggcggc ggcacctaca tcagccgcct
    10621 gctcggctcc ctcgaggacg ccgaaaccga cccgatcgcc accgccaccc gcatccgtca
    10681 ggtcagctcg ttcaccctgt ggggcgcgct ggccggcggc gccgtgatcg gcctgctggg
    10741 gctggtcttt gccaccccca tcgccgtcgc ggtcggcgcg agcggcgaat cgctggcacc
    10801 caccgccgtc tacatcggcg ccatgttcgc cttcaccccc ctctacgtgg cctgcttcgc
    10861 gctcgaacag gtggtgcgcg ccgagggtgc cgcggtggcg tccatgaccg gggtgatcgc
    10921 ctccaccatc gccaacctgg ccttcgacgt gctgttcatc ctggtgctcg gctggggcgt
    10981 gctcggcgcc ggcatcgcgc tcggcctgtc caacctggtg atggtcggct actacgtgtg
    11041 gtggctggtc cgccgcagct cggtggccgc gctcggcccg cgctggttcc gcgccgaccg
    11101 cgagatgctg cggacggtgt tcggcgtcgg cgtctcggaa ctgttgcagt cgagcttcct
    11161 gatcgtcacc accctggtga tgaactggat cgcgatcggc tacggggacg ccctgctggc
    11221 ctcgatgggc gttgcgctgc ggatttcgca gttgccggag atgatctgca tgggtgtgtt
    11281 catgggtgcc gtgccgctgt tcgcctacgc cttcggggcc ggcaaccatg cccggctgca
    11341 ccgggccatc ggcggcgccg cggtcggcat tctcggcttc accctggtgt tctcgggact
    11401 ggtcttcgcg ttccgcgacc aggtgttcgg cctgttcagc gccgacccgg cggtgctctc
    11461 ggacggcacc gcgatcctca ccgcgatgct ggtggccacc ctgttcaacg gcatcaccgg
    11521 cctgctgatc gcggtcttcc aggccaccga gcagatgcgc aacgccacca tcatgtcgat
    11581 cgcccaagga gtgctgttca ttcccgtggt gctgctcggc aacgcctggt tccacctcga
    11641 cggggtgatc tggtcgatga ccgtcaccga actactcacc ttcgccctcg gcggcctgct
    11701 gttcctcacc tcgcggcggg ccctggacgc cgccccgtcc gccgaatcga gtgcagcggc
    11761 cgccgaactg gtggacgggg ccgtcgccac cgcttgacca cctacaacca aatggttgta
    11821 tgttgtccct gtgatcgaga ctgacgagga tcgggcggac gcgctcttcc acgccctggc
    11881 cgaccgcacc cggcgcgaca ttctgcgccg ggtgctggcc ggtgagcact cggtcagcac
    11941 gctggccaag cactacgaga tgagcttcgc cgccgtgcag aaacacgtcg cagttctcga
    12001 gcgggcgggc ctgctgacca agcgacggca gggtcgcgag gccctggcca gcggcgacgt
    12061 gcaggtggtc cgcgccgcga accagctgct cagcgaactc gagagtgtgt ggcgcgggcg
    12121 catcgcccgc atcgatgcgt tgctcgtcga gaactcgcag catgacaacg cacacagcca
    12181 gggcgccgac agccgcccgt cgatcccccc accagtccca cgacaccagc acaagcccac
    12241 acccgttgac cgatccgagg agtaagttgc catgcccgtt gtcgatgtga tcaagaacct
    12301 ggacggcccg tccctcacta tcgtggccga cttcgccgca cccgtggaac gggtctggca
    12361 gctctacgcc gacccgcgcc aattggagca gatctggggg ccgccggagt acccggcgac
    12421 cgtcgtccag cacgatctga ccccgggcgg gcgggtcacg tacttcatga ccggcgccgg
    12481 gggcgaccgg cacttcggct actgggacgt cgccgaggtg accgcacccg agcacttcag
    12541 cttcgcggac ggcttcgcca acgatgagtt cgagcccaac accaatctgc cggtttcgca
    12601 caacagctac acgttcgggg cgcaccaagg tggcacccgt gccacgtacg tctcaacgtt
    12661 cgccagcgcg aacgacctgc agcaggtgct cgacatgggc gtcgaagagg gcaccatcgc
    12721 cgccaccaac cagatcgacc gcctgctcgc actcgactga ggctgaccca ccccgctgaa
    12781 gctgattcgg agctactagg gtggggcgtg gcggagcgtc cgtccgagca gaccgaagga
    12841 gcaactgcat ggcaacgcag agcaaggccc aggcccactg ggaaggcagc ctgatggagg
    12901 gcgccggaag cgtcgacctg gtcacctccg gcgtgggtca cttcgacctc aaatgggcca
    12961 agcgcgccga ggcctccgca ggcgccacga accccgaaga gctgatcgcc gcggcgcacg
    13021 cgtcgtgtta ctcgatggcg ctgtcccacg ccctggccgg caacggcacc cccgcggagt
    13081 cgatcgacac caccgcggtg gtcggctttc agcctggcgt cggcatcacc ggcagcgacc
    13141 tgacggtcaa ggccaaggtg ccgggactca ccgagaacga gttccagaag ttcgcggacg
    13201 aggccaagac cggctgcccg gtctcggcgg ccctgaccgg cattccgatc acgctgaccg
    13261 cagagttcat cagctgatat ccccggttga ggaatcgctg gtcgagcgtg tcggggcccg
    13321 tgccaccagg ggtttcgacg ttcaggctca accaccgggc cgcgcattat ctgcgcttcc
    13381 ttgatcgagc acccgcgcga gggcgtccgt ttgggcgccc tcgacgcatg tgcgcgcatg
    13441 gcaacctccg ataggtggac gtcggcgagg gccgatgcgc cgacgtcggg ggctggatgc
    13501 gcggccggcc tgcgagggac gaaacgcggc agccgaccgg cggcggcggc tcgcgacgca
    13561 ctctcacccg gcgtcagcgt ggccgagtcg gctgcgtcac aatgaccgca tgacgattgt
    13621 gcccggcgcc gagccgatca gtgtggacgg atccgacgtc ggggtgctgc tgtgtcacgg
    13681 ctacccgagc accccgcagt cgatgcgtcc ctgggccgag cacctggcgg cggccggcta
    13741 cacggtgcgg gtgccgttgc tgcccggcat gggcacccat tggagggatc tgaacgcaac
    13801 cggctggcag gactggtacg gcgagatcga ccgggctcac gccgaactca gcgcgcgctg
    13861 ttcgacagtc ttcgcgttcg gcttgtccat gggcggcctg ctggccacca aactggcgca
    13921 ggagaagccg gacctggccg gcctggtgct ggtcaacccg atctttgcgc acaacaaccc
    13981 ggcgttgaag attctgccgc tgttgcgcca cgtggtgccg gccctgggcg gactggccgg
    14041 cgacatcaag aagccgggcg tgatcgagtt ggcctatgac cgcaacccgc tgcaggcgat
    14101 gcacagccaa accaaactgt ggcgcatagt cgtcgaagac ctgccgcaac ttcgcttgcc
    14161 ggtgctgctg atcacctcaa gcgaagacca cgtcgtaccc ccgctgtcgt ggcagacatt
    14221 cc
//
LOCUS       DAOSUG010000009        14062 bp    DNA     linear   ENV 10-JUN-2024
DEFINITION  MAG TPA_asm: Micropruina sp. isolate AMR_MDS_2195
            NODE_2613_length_14062_cov_4.739630, whole genome shotgun sequence.
ACCESSION   DAOSUG010000009 DAOSUG010000000
VERSION     DAOSUG010000009.1
DBLINK      BioProject: PRJNA925477
            BioSample: SAMN32864262
            Sequence Read Archive: SRR4236673
KEYWORDS    WGS; ENV; Metagenome Assembled Genome; MAG; Third Party Data; TPA;
            TPA:assembly.
SOURCE      Micropruina sp. (wastewater metagenome)
  ORGANISM  Micropruina sp.
            Bacteria; Bacillati; Actinomycetota; Actinomycetes;
            Propionibacteriales; Nocardioidaceae; Micropruina.
REFERENCE   1  (bases 1 to 14062)
  AUTHORS   Abdulkadir,N., Saraiva,J.P., Zhang,J., Stolte,S., Gillor,O.,
            Harms,H. and Rocha,U.
  TITLE     Genome-centric analyses of 165 metagenomes show that mobile genetic
            elements are crucial for the transmission of antimicrobial
            resistance genes to pathogens in activated sludge and wastewater
  JOURNAL   Microbiol Spectr 12 (3), e0291823 (2024)
   PUBMED   38289113
REFERENCE   2  (bases 1 to 14062)
  AUTHORS   Abdulkadir,N.
  TITLE     Direct Submission
  JOURNAL   Submitted (24-MAR-2023) Environmental Microbiology, Helmholtz
            Center for Environmental Research -UFZ, Permoserstr 15, Leipzig
            04318, Germany
COMMENT     The annotation was added by the NCBI Prokaryotic Genome Annotation
            Pipeline (PGAP). Information about PGAP can be found here:
            https://www.ncbi.nlm.nih.gov/genome/annotation_prok/
            
            ##Genome-Assembly-Data-START##
            Assembly Method        :: SPAdes v. 3.11.1
            Genome Representation  :: Full
            Expected Final Version :: Yes
            Genome Coverage        :: Not Applicable
            Sequencing Technology  :: Illumina MiSeq
            ##Genome-Assembly-Data-END##
            
            ##Genome-Annotation-Data-START##
            Annotation Provider               :: NCBI
            Annotation Date                   :: 05/04/2023 04:57:10
            Annotation Pipeline               :: NCBI Prokaryotic Genome
                                                 Annotation Pipeline (PGAP)
            Annotation Method                 :: Best-placed reference protein
                                                 set; GeneMarkS-2+
            Annotation Software revision      :: 6.5
            Features Annotated                :: Gene; CDS; rRNA; tRNA; ncRNA
            Genes (total)                     :: 2,776
            CDSs (total)                      :: 2,750
            Genes (coding)                    :: 2,714
            CDSs (with protein)               :: 2,714
            Genes (RNA)                       :: 26
            tRNAs                             :: 24
            ncRNAs                            :: 2
            Pseudo Genes (total)              :: 36
            CDSs (without protein)            :: 36
            Pseudo Genes (ambiguous residues) :: 0 of 36
            Pseudo Genes (frameshifted)       :: 2 of 36
            Pseudo Genes (incomplete)         :: 32 of 36
            Pseudo Genes (internal stop)      :: 2 of 36
            ##Genome-Annotation-Data-END##
FEATURES             Location/Qualifiers
     source          1..14062
                     /organism="Micropruina sp."
                     /mol_type="genomic DNA"
                     /submitter_seqid="NODE_2613_length_14062_cov_4.739630"
                     /isolate="AMR_MDS_2195"
                     /isolation_source="Activated sludge of municipal
                     wastewater treatment plants"
                     /db_xref="taxon:2737536"
                     /environmental_sample
                     /geo_loc_name="Canada"
                     /lat_lon="49.25 N 123.1 W"
                     /collection_date="2015-06-16"
                     /metagenome_source="wastewater metagenome"
                     /note="metagenomic"
     gene            complement(<1..938)
                     /locus_tag="PLF56_00610"
     CDS             complement(<1..938)
                     /locus_tag="PLF56_00610"
                     /EC_number="4.1.1.32"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_009213061.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_function: GO:0004611 - phosphoenolpyruvate
                     carboxykinase activity [Evidence IEA];
                     GO_process: GO:0006094 - gluconeogenesis [Evidence IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="phosphoenolpyruvate carboxykinase
                     domain-containing protein"
                     /protein_id="HOQ52093.1"
                     /translation="MSVDAQLVALDAPAGAPEALVNWVAGVAELTQPEAVYWCDGSEA
                     ERDRFYAEMVASGTLIKLNPDKRPNSYLARSSASDVARVESRTYICSANESDAGPTNN
                     WADPAEMKQTLARVFHGCMRGRTMYVVPFSMGPLGGNISKLGIEITDSPYVVVSMRIM
                     TRMGAEPLARISAGEYWVPAVHSVGYPLIDADGNRRDDIAWPCNDDKYITHFPETREI
                     WSYGSGYGGNALLGKKCYALRIASVLAHDEGWLAEHMLILKITGPDGRVYHLTAAFPS
                     ACGKTNLAMLRPTIPGWKVETIGDDIAWMRPGPDGRL"
     gene            1098..1170
                     /locus_tag="PLF56_00615"
     tRNA            1098..1170
                     /locus_tag="PLF56_00615"
                     /product="tRNA-Arg"
                     /inference="COORDINATES: profile:tRNAscan-SE:2.0.12"
                     /note="Derived by automated computational analysis using
                     gene prediction method: tRNAscan-SE."
                     /anticodon=(pos:1131..1133,aa:Arg,seq:acg)
     gene            1402..3213
                     /locus_tag="PLF56_00620"
     CDS             1402..3213
                     /locus_tag="PLF56_00620"
                     /inference="COORDINATES: ab initio
                     prediction:GeneMarkS-2+"
                     /note="Derived by automated computational analysis using
                     gene prediction method: GeneMarkS-2+."
                     /codon_start=1
                     /transl_table=11
                     /product="hypothetical protein"
                     /protein_id="HOQ52094.1"
                     /translation="MGGYRRVDDERSAVALSEFLNGGSRKRPVVVVTIPASRTEPWID
                     VDAIAREAGNLADVYLMPTGDVSWKFSDRMADGTQVFGGAGRVYPIGHEWATDLTKSP
                     LRFAWSEQDGEKATRQLVSDVFRAAARGLARALPTRPLREVDGVVKLLVAGRALVSIG
                     NAMAAVAQELTVEDVPIERLMTVGQRVSGRYDADTNRLDVTTSLRPADDALTGYVLGD
                     VVLARVVSVRADTAELMLFPKTTTPAITVAVSLADVTLNPLDDLRQLMTVGEVVAARV
                     TATSPRWALVLNDVDDDEPIRSAPSVLTGGPPWLLEEPVGIAEAAPPLAPPAPVRPSS
                     FPGPSVEESEPVEPAPSRPAPMRPSPALFDRNRPRQAAPPASLTPPSGAQPSEGTKSL
                     LVKIDGLTSEVNRHKREANELRTQLLDASDEREQLRYLLEQAERRANRSEHELKVARA
                     RLRKVGNSKASAERAREPQFADHEQGFRYLVLTRWATRTLPSEQAQRPLLNYAIGPEF
                     LGSLAELQGITEAKVADVVFEIVTGLAPQIAGRQVHQLRTGAGGNDPFRVREDGARAY
                     RASLQVDTPSARRIHYWILPGGQIELAQVDTHDQFGV"
     gene            3944..4618
                     /locus_tag="PLF56_00625"
     CDS             3944..4618
                     /locus_tag="PLF56_00625"
                     /inference="COORDINATES: protein motif:HMM:NF012696.2"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_function: GO:0004089 - carbonate dehydratase activity
                     [Evidence IEA];
                     GO_function: GO:0008270 - zinc ion binding [Evidence IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="carbonic anhydrase"
                     /protein_id="HOQ52095.1"
                     /translation="MVVPAQVPDWMRPQGLPHQRMPSAQALQRLQAGNVRYCQQRAGN
                     APPPGDATPAGFPFALVVGCLEPEAAGEHLFAEKPGAIEVVRNAGPSLGSDALAAIEY
                     AALVQNVGVVLVLAHKGCGVLTAAAAARAANGVALPGVLKQWVPLAAAGTRLGDPAVG
                     HVRALRWELRRTDSLLGLTRSGQVKIAAGVLDPDTGAIQFLPDEPDEVEDDSASTPVN
                     TITYGS"
     gene            complement(4752..6296)
                     /locus_tag="PLF56_00630"
     CDS             complement(4752..6296)
                     /locus_tag="PLF56_00630"
                     /EC_number="6.5.1.1"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_013862076.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_function: GO:0003677 - DNA binding [Evidence IEA];
                     GO_function: GO:0003910 - DNA ligase (ATP) activity
                     [Evidence IEA];
                     GO_function: GO:0005524 - ATP binding [Evidence IEA];
                     GO_process: GO:0006281 - DNA repair [Evidence IEA];
                     GO_process: GO:0006310 - DNA recombination [Evidence IEA];
                     GO_process: GO:0071897 - DNA biosynthetic process
                     [Evidence IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="ATP-dependent DNA ligase"
                     /protein_id="HOQ52096.1"
                     /translation="MLLADLVATSAQVAATRSRLAKVAALVEALNAAEPSEIEIVASY
                     LSGSLRQRRTGVGWRSARATVPPAQQPTLNLTEVDAAFETLSALSGPGSQLARKTQLD
                     ALMARATAAEQQWLAGLITGETRQGALDGLMVDAVIRASGLPAADVRRAVMLAGTTPS
                     VAFAALAGGAEAVAAIGLVVGRPIKPMLAASAKSVAEAMTSLGGTVAVEAKLDGIRIQ
                     AHRDGAVVRLFTRSLDEVTDRLPEVADAVARLPVQRVILDGEALAIGSDGRPRPFQET
                     SSRSMTQDAGAVRERTPMTPFFFDLLALDDEQLLDLPAAERADRLAALVPETARVGRL
                     VTDDPAEAETFTQAVLASGHEGVVIKALDAPYEAGRRGSSWIKVKPVHTLDLVVLAVE
                     WGSGRRRGWLSNIHLGARDPATGGFVMLGKTFKGMTDAMLAWQTERFLDLETSRDDWT
                     VRLRPEQVVEIAFDGLQRSSRYPGGLALRFARVLRYRDDKTADEADTIDQVRALAGPA
                     HAPDQQ"
     gene            6421..7335
                     /locus_tag="PLF56_00635"
     CDS             6421..7335
                     /locus_tag="PLF56_00635"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_011756281.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="DNA-3-methyladenine glycosylase 2 family
                     protein"
                     /protein_id="HOQ52097.1"
                     /translation="MQPIVREWRPAWPCPVGQVWSQWRRGAGDPTYLVDRGRIWRGIR
                     TPLGPATLAVQPLNTDGVIVAEAWGEGADWVLDELPGMLGAHDDPRGFVPRHPQVAQA
                     LKERPHWRIGRGNLVWQALLPAVIEQKVTGQEAFAGYRRLVHRHGERAPGPGVALQLW
                     VPPGPQTVRMIPSWEWLKLHIDPARSRTLVRCAQVAPALERTLELPNDEADARLRSIA
                     GIGVWTSAEVRFRAHGDADAVSFGDYHVPNEIGHALLGHDLDDAGLAELLEPYRPHRH
                     RVQALLGTRRWRERHGPRMAPRTHLPAR"
     gene            complement(7479..7817)
                     /locus_tag="PLF56_00640"
     CDS             complement(7479..7817)
                     /locus_tag="PLF56_00640"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_011754442.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="YciI family protein"
                     /protein_id="HOQ52098.1"
                     /translation="MPDYLIYFNQQWVGDHSEDWFRSREKPSNAVVDEMKAAGVYVYA
                     AGVEVNGPVYAADATSGEVVVTDGPYTDTPQRLGGFTIVSVADDEAAKHWAGKIAVGC
                     GWPQEVRRIG"
     gene            complement(7867..9888)
                     /locus_tag="PLF56_00645"
     CDS             complement(7867..9888)
                     /locus_tag="PLF56_00645"
                     /inference="COORDINATES: ab initio
                     prediction:GeneMarkS-2+"
                     /note="Derived by automated computational analysis using
                     gene prediction method: GeneMarkS-2+."
                     /codon_start=1
                     /transl_table=11
                     /product="hypothetical protein"
                     /protein_id="HOQ52099.1"
                     /translation="MTQPPRIPRRTLLAAAGAGAVLTGLGAAPSARAATRLTNLSHLD
                     ELTTTVRLRHTAAHSTYRLPQEPAVGLLWVYADVQADGGYRPVGGGSYDAASDTWGQG
                     AYDSDDIARAAVVYLREWRATGSSHARRQAYQQLRGLAYFQTLTGEHAGEFLLWMQPD
                     GTLNPTPTPPDNPNPADAGESYWTARALWAFGEGYVAFARHDPAFAAFLRRRMELAIA
                     AVERDTLVRYGTYHDLHGVRVPAWLITQGADATSEALLGLAPYAAATGSAPARRACRR
                     LARGVAEFAAGSTTQWPFRALLPWAESLDDWHAWGSEMAQGLAAAAKALRDPGLLRPA
                     IGDTAGFTAQLLTATGPDNGWLPVPLEKVQIAYGADARMRACYEVGRVAGREGITRLA
                     GIAAGWFFGANRAGVPTYDPATGVTFDGVEADGRLNRNSGAESTIHGLLTMQVLDAAP
                     ALARLARASARIGVRDGLTVIEAEAGTLGGSARVESSDPAWTGESLWSGKRVVGGDGA
                     TITWTLPASAGALLVQPVVALEHGSAASVRFTAGDRRLGRVRFGDVGPQGDAASPLRL
                     TPVALDGLASAGTTVTATVQGAGSARLDALLVMPEIARLEASGSTGAVVLLTSKSERR
                     QLRTVSLPGGRLQVESYDRNGMRTRQATRGGRVVVEPGGFTLVFVGTGS"
     gene            complement(10036..11085)
                     /locus_tag="PLF56_00650"
     CDS             complement(10036..11085)
                     /locus_tag="PLF56_00650"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_012919291.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="glycosidase"
                     /protein_id="HOQ52100.1"
                     /translation="MTDFPYRLTRAGVVMRPDPNDAREAEGVLNPAAAWAPDGRLKLF
                     PRVVARGNFSRIAVADVVIGDGVPIGVQRRGIALAPDRAFERGSDHGGVEDPRITFVS
                     ALGLYLMAYVAYGPAGPRAALATSPDLIDWTRLGPVQFRYDDDLGVDLNLFANKDLAF
                     FPDPVTGPDGSACLGFLHRPMFELPGQQVELPAGSTDARAAIWLSYVRLADAIADPDA
                     LLRPFGHRELARSQADWEHLKIGGGPPPVRVPQGWLLLYHGVSGTMSSNPFEPQKNVH
                     YAAGGLLLDADDPSRVIARTTSPLLSAETADERVGIVANVVFPTAIVEIERALFTFYG
                     MADEAIGVARIERVD"
     gene            complement(11116..12009)
                     /locus_tag="PLF56_00655"
     CDS             complement(11116..12009)
                     /locus_tag="PLF56_00655"
                     /inference="COORDINATES: protein motif:HMM:NF012738.2"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_process: GO:0008643 - carbohydrate transport [Evidence
                     IEA];
                     GO_process: GO:0055085 - transmembrane transport [Evidence
                     IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="carbohydrate ABC transporter permease"
                     /protein_id="HOQ52101.1"
                     /translation="MPVAAVPAGASTAPSGRGPAAESVGAPRNHGLAVASYALMALVI
                     VIYLYPLLFLVNTALKSNNEFLVNPTGVVQSPQFGNFAAAWQQGNFASYMLNSVLYTF
                     VAAALGTVMSLMIAVPVARGYVKHPKVWMGLFASMLFLPNTLVTVFQLALRLNLYDTP
                     LGYILIMASGVGVGPLLIAGYLNSIPREIDEAAAMDGCGYLRYLFTFLPKLMMPVLST
                     AFILQAIGVWNDIILATILFSSQEHWPVTLGLFNFKGTYTSEWSLLSAATMIVAAPLL
                     LVYLFLQRYLVASVVGGAVKG"
     gene            complement(12015..12926)
                     /locus_tag="PLF56_00660"
     CDS             complement(12015..12926)
                     /locus_tag="PLF56_00660"
                     /inference="COORDINATES: protein motif:HMM:NF012738.2"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_function: GO:0042626 - ATPase-coupled transmembrane
                     transporter activity [Evidence IEA];
                     GO_function: GO:0140359 - ABC-type transporter activity
                     [Evidence IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="sugar ABC transporter permease"
                     /protein_id="HOQ52102.1"
                     /translation="MSNSYIVFGRRWTAQLTFAIGMALIVVFSLIPAVGIVVISFTDI
                     RSLPFLPVRWVGLDNYRTFFSSAQLAYNVAALQNTFVFAFAVTIFQNVIALLIAVLLN
                     QRLKGRNFARAVVFLPTILGVTVIGLIFSLIFNPSAGPAAQLWGMFGASSAFFGDPSL
                     AMTLVILVQVWSGIGVAVVIYLAGLQAIPQELYEVADIDGATGGQKLRHVTIPLLAPS
                     VTANTLLCIIGALQSYQLIYVLTGPINPATQVLSLAIFTQGFGGPQGGAVQSQGYAAA
                     IAVVQFIIVAIISLATLAYLRRRETAL"
     gene            complement(13005..>14062)
                     /locus_tag="PLF56_00665"
     CDS             complement(13005..>14062)
                     /locus_tag="PLF56_00665"
                     /inference="COORDINATES: protein motif:HMM:NF024808.2"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=3
                     /transl_table=11
                     /product="extracellular solute-binding protein"
                     /protein_id="HOQ52103.1"
                     /translation="GYRHRDLDTRAGTIDVGLANPRQLPSYVPESNKGDDARLAEAGG
                     FVDLTDQAFMKKFNASVLDKITYNGRNYTVPTGLSYYTGLTYNKKIFADNNITVPTTW
                     SDFIKVLDQLKTKGITPISIGGKDTAGIVMLSVVQSLYPTAQNKDDLAKGLYEHTVAL
                     NQGQQLEVLKKVQQIYTYGQPNFAGSNYNQMTSEFLTGKAAMISDGTWNVGSLREADK
                     VDFGYFPLPASDNAADNAYLGGKVELSLAVPSNAKNKDAAMAWLAFFADNYKLFNDEA
                     GFAPSQEGVEGDPFYAEIATYTKQFEPAWDTIWIANTKAGQAAALPFNWSAVKPMGSM
                     DAQAAADASQKDWEAGK"
BASE COUNT         2206 a         4805 c         4876 g         2175 t
ORIGIN      
        1 agccgtccgt cggggccggg acgcatccag gcgatgtcgt cgccgatggt ttcgaccttc
       61 cagcccggaa tggtcggacg cagcatggct aggttggttt tgccgcaggc cgatgggaac
      121 gcggcggtca ggtgataaac ccggccgtcg ggaccggtga tcttcagaat cagcatgtgc
      181 tcggccagcc agccctcgtc gtgggccagc accgacgcga tgcgcagcgc gtagcacttc
      241 ttgcccagca gcgcgttgcc gccgtagccc gagccgtagc tccagatctc gcgggtctcg
      301 ggaaagtggg tgatgtactt gtcgtcgttg cacggccagg cgatgtcgtc gcgccggttg
      361 ccgtcggcgt cgatcagcgg gtagccgacc gaatggacgg cgggcaccca gtactcgccg
      421 gcgctgatgc gggccagcgg ctcggcgccc atccgggtca tgatgcgcat gctgaccacc
      481 acgtagggcg agtcggtgat ctcgatgccg agcttggaga tattgccacc cagcgggccc
      541 atcgagaacg gcaccacgta catcgtgcgg ccccgcatgc agccgtggaa cacccgtgcc
      601 agggtctgtt tcatctcggc cgggtcggcc cagttgttgg tcgggccggc gtccgattcg
      661 ttggcggagc agatgtaggt gcgcgactcg acgcgggcca cgtccgaagc ggaggagcgt
      721 gccaggtagc tgttcggacg cttgtcgggg ttcagcttga tcagggtgcc ggaggcgacc
      781 atctcggcgt agaaccggtc acgctcagcc tcggaaccgt cacaccagta gacggcctca
      841 ggttgggtga gttcggcgac accggccacc cagttgacca gggcttcggg tgcgccggca
      901 ggggcatcga gagctaccag ttgggcatcg accgacatct cggactcctt cgacccgcta
      961 ccttgcgggc atcgcttcca agacaccagt ctgccttacc ccaaagggtg gcatggccgg
     1021 ttgggctcag ttggacgcat ctcggtgcgt caactaaagt gaaccctcgg tggtcggtag
     1081 gttcgatcat catctgagcg cctgtagctt aacggataga gcatctgact acggatcaga
     1141 aggttggggg ttcgaatccc tccaggcgcg cgtaacagga ccggctccac ccggtcctgt
     1201 ttctgcgttc ggtgacggct ctgccccagg gggtgtcgag ggggcgtgct gacagttcct
     1261 tgcttcgagc atcgtgcctc gcgcaagcaa ctgggccgtg cagggaacgg ccgcagatgt
     1321 gaggcatttt cgaacctggg aggtcctcga ctcacacccg ctcacgacac actgtctgtg
     1381 atcgaaccta agcttcggat cgtgggtggc tatcgacgtg tcgatgacga gcgttccgcc
     1441 gtcgcgctgt ccgagttcct gaacggaggt tcgcgaaagc ggcccgttgt cgtcgtgacg
     1501 atcccggcca gccgtacaga gccctggatc gacgtagacg cgatcgcacg cgaggcgggg
     1561 aacctggccg atgtctacct catgcctacc ggcgacgtct cgtggaagtt ctctgaccgg
     1621 atggccgacg gcacccaggt cttcggcggt gcaggacgtg tgtacccaat cggccacgag
     1681 tgggcgacgg acctgacgaa gtctccgctc cgcttcgctt ggagcgagca ggacggcgag
     1741 aaggccacgc ggcaacttgt ctcggacgtg tttcgcgccg ccgcgagagg cctcgctcgg
     1801 gcgctgccta cccgacctct ccgcgaggtc gacggggttg tcaagctgct cgttgcaggg
     1861 cgggcgctcg tcagcatcgg gaacgccatg gccgctgtcg cccaggaact cacggtggag
     1921 gacgttccca ttgaacgtct gatgacggta gggcagcggg tcagcgggcg gtatgacgcc
     1981 gacacgaacc ggctcgacgt aacgacgagc ctccgccccg ccgacgatgc actcaccggt
     2041 tacgtgttgg gcgacgtcgt gctcgccagg gtcgtcagcg tgcgagccga caccgcagag
     2101 ctgatgctct tcccgaagac cacgaccccg gcgataacgg tcgcggtgag tctcgccgat
     2161 gtcaccctga accctctcga cgacttgcgc cagctcatga ctgtcggtga ggtcgtagcc
     2221 gcgcgggtga ccgcaacctc gccccggtgg gcgctggttc tcaacgacgt ggatgacgat
     2281 gaaccgatcc gctccgctcc ctcggttctc accggggggc cgccctggtt gcttgaggag
     2341 ccggtcggca ttgcggaggc tgcgcctcca ctcgctccgc cggcgcccgt gcgtccgtcg
     2401 tcgttcccgg ggccatcggt cgaggagtcc gagccggtcg agcctgcgcc ctctcggccg
     2461 gcgccgatgc ggccaagccc ggcgctgttc gaccgcaacc gcccgcgtca ggctgcgccg
     2521 ccggcgtccc ttacgccgcc atccggtgcg cagccgtccg aggggacgaa gtcgctgctg
     2581 gtgaagatcg acgggctgac cagcgaggtc aaccggcaca agcgcgaggc gaacgaactg
     2641 cgcacgcaac tcctcgatgc cagcgacgaa cgggagcaac tccgctatct gttggagcag
     2701 gcggagcgcc gcgcgaaccg gtccgaacac gagctgaagg tcgcccgagc ccgcctccgc
     2761 aaagtcggga actcaaaggc gtccgcagag cgcgcccgcg aaccccagtt cgccgaccac
     2821 gaacagggct tccgctacct cgttttgacc cgctgggcca cgcgtacgct gcccagcgag
     2881 caagcgcagc gtcccttgct caactacgcc atcggcccgg agttcctagg ctcactcgcc
     2941 gaactgcagg ggatcactga agcgaaggtg gccgacgtcg tattcgagat cgtgacaggc
     3001 ctggccccac agatagcggg ccgccaggtc catcaacttc gcaccggagc aggcggcaat
     3061 gatccctttc gagtccggga agacggcgcc cgggcgtacc gggccagtct ccaggtcgac
     3121 actccctcgg ctcgccgcat ccactactgg atcctgcccg gtggacagat cgagctcgcc
     3181 caagtcgaca cacacgacca gttcggcgtc tgatctgtcg ccgagcgccc accaccggga
     3241 tgggcggccc ttgcttgcct gactgcacct atcgacgccg cattgtgtgc gcaacttctt
     3301 accgtcgagt aggccggcgc gagttgatgc ttgaacgcac tggtgaggac acgcccaagt
     3361 aggacgcatg cagagtgcgc gcttgtggtt gcatgccatt cggcccattc ggcccattcg
     3421 gccccgaccg cagatcgaca gtccatcgtc cgcgacgaaa ctcgttgcgg gacgtgagcg
     3481 agccgcacgg gcggggagtc aaccaaggat cgaccaggca tctgtcatca ctgctgggct
     3541 agttggcggc caagcttccc gtgacatcga cggctcacca tggtgctgaa gcggcttggt
     3601 gagggtgtgt tccgccaact cttggcggcg atggatgcac cgacgttggc cggcgcactg
     3661 gcggccatga atccccgagg cgctgaagaa cctgatcccg ctactgcttc ctgaccaaat
     3721 cgcagcgata ctcagcgtcc tacaaggacc ctgggcccag cggagccaat acagcccgga
     3781 tcctgcgagg gccgccgtag cgacccgcac cagatacccg ccccgcccgc gcgggggtga
     3841 accccagttg gtggacgccg tgaccgcacc cggcaggtcc accccacacg aaccttctct
     3901 acatcatctc gacaagcgct ttcacgctca ctagcctcgg ctcatggttg tccctgctca
     3961 ggtgcccgat tggatgcgac cgcagggcct tccccaccaa cgcatgccgt cggcgcaggc
     4021 gttgcagcgc ttgcaggcgg gaaacgtgcg gtactgccag cagcgagccg gtaacgcacc
     4081 tccccccggc gacgccaccc ccgccggctt tcccttcgcc ctcgtggtcg gttgcctcga
     4141 gcccgaggcg gccggcgaac acctgttcgc ggagaagccg ggggcgatcg aggtcgtccg
     4201 caacgccggg ccgagcctgg gttcggacgc cctcgccgcc atcgagtacg ccgcgctcgt
     4261 gcagaacgtc ggggtggtgc tcgtgttggc gcacaagggg tgcggcgtgc tcaccgccgc
     4321 tgccgccgca cgtgccgcca acggggtggc actacccggg gtgctgaagc agtgggtgcc
     4381 gctggccgcg gcgggcacgc ggttgggcga cccggcggtc ggtcatgtgc gcgcgttgcg
     4441 gtgggagttg cggcgcaccg actcgctgct tgggctgacc cgctcggggc aggtgaagat
     4501 cgcggccggt gtgctcgacc ccgacaccgg cgccattcag ttcctgcccg acgagccgga
     4561 cgaggtcgag gacgacagtg cgtccacgcc cgtcaacacg atcacgtacg gcagctgacc
     4621 tcagccggct ccgggcccgg tgggcgcgcc tggtcgggaa gcatcacggc ccgcccacac
     4681 gtgcaatctg gtgctggtcc atgcccattc ctgcggccga gcggcgaagc cggggaccca
     4741 ttcgctgcgc ctcactgctg gtcaggggcg tgtgcaggcc cagcgagtgc gcgcacctgg
     4801 tcgatcgtgt cggcctcgtc ggcggtcttg tcgtcgcggt agcgcagcac ccgcgcgaac
     4861 cgtaacgcca gaccccccgg gtagcgtgac gaccgttgca agccgtcgaa ggcgatctcg
     4921 accacctgct cggggcgcag ccgcaccgtc cagtcgtcgc gacttgtttc gaggtcgagg
     4981 aaccgctcgg tctgccaggc cagcatcgca tcggtcatgc ccttgaaggt cttgcccagc
     5041 atcacgaagc ccccggtggc cgggtcgcgg gcaccgaggt gaatgttgct gagccagcca
     5101 cgacgtcgtc cggaccccca ctcgacggcc agcaccacca ggtcgagggt gtgcaccggc
     5161 ttcaccttga tccaggacga tccgcgccgc ccggcctcgt acggcgcgtc gagggccttg
     5221 atcaccacgc cctcgtgccc cgacgccagc acggcctgag tgaaggtctc agcctcggcc
     5281 gggtcgtcgg tgaccagccg ccccacccgc gcggtctcgg gcaccagcgc ggccaaccgg
     5341 tcggcgcgct cagcggccgg caggtcgagc agttgctcgt cgtcgagggc cagcaggtcg
     5401 aagaagaagg gggtcatggg ggtgcgttcg cgcaccgcac ccgcgtcttg ggtcatcgaa
     5461 cgggaggagg tctcttgaaa gggtcgcgga cggccgtcgg acccgatcgc cagcgcctcg
     5521 ccgtcgagga tcacccgttg caccggcagc cgcgcgaccg cgtcggccac ctcgggcagg
     5581 cggtcggtga cctcatcgag gctgcgggtg aacagccgca ccaccgcacc gtcgcggtgg
     5641 gcctgaatgc ggatgccgtc gagcttggcc tcgaccgcca ccgtgccgcc caacgaggtc
     5701 attgcctcgg ccaccgactt cgcgctggcg gccaacatcg gcttgatcgg ccgacccacc
     5761 accaggccga tggcggccac cgcctcggcg ccgccggcca gggcggcgaa ggcgacggac
     5821 ggcgtggtgc cggccaacat caccgcacga cgaacgtcgg ctgccggcaa cccactcgcc
     5881 ctgatcaccg cgtcgaccat caggccgtcc agtgcgcctt gccgggtctc gccggtgatc
     5941 aacccggcaa gccactgttg ctcggcggcg gtggcgcggg ccatgagggc gtccagctga
     6001 gtcttgcgcg ccagctgcga accggggcct gacaacgccg acagcgtctc gaacgcggca
     6061 tcgacctcgg tgaggttgag ggtgggctgc tgtgccggcg gcacggtggc tcgcgcggag
     6121 cgccagccga cgccggtgcg gcgctggcgc agcgacccgg agagatacga ggcgacgatc
     6181 tcgatctcgg acggctcggc cgcgttcagc gcctcgacca gcgccgccac cttggccagc
     6241 cgggaccggg tggcggccac ctgcgcggag gtggcgacga gatcggccag caacatgacg
     6301 tccattctgc acgtgggctc tgacagttcc tcttgcggcg gcgatcggaa gggccgccgg
     6361 acggacgtgg gcccggggtc gcgcccggaa tcgccatcgg cgacgggtag gttgaccctc
     6421 gtgcagccga tcgtccgtga gtggcgtccg gcctggccgt gcccggtggg tcaagtctgg
     6481 tcgcagtggc gccggggggc cggcgatccc acctacctgg tcgatcgcgg acgcatctgg
     6541 cgcggcatcc gcaccccgct cgggccggcg acccttgcgg tgcagcccct gaataccgac
     6601 ggcgtgatcg tggccgaggc gtggggcgaa ggcgccgact gggtgctgga cgagctgccc
     6661 ggcatgttgg gcgcgcacga tgacccgcgc ggcttcgtgc cgcggcaccc acaggtggcg
     6721 caggcgctga aggaacgtcc gcactggcgc atcggccgcg gaaacctcgt ctggcaagcg
     6781 ctgttaccgg cggtgatcga gcagaaggtg accggtcagg aggcgttcgc ggggtaccgg
     6841 cggctggtgc atcgtcatgg cgaacgcgcg cccgggccgg gcgtcgccct gcagctgtgg
     6901 gtgccgccgg gcccgcagac cgtccggatg attccgtcgt gggagtggct caagctgcac
     6961 atcgacccgg ctcgctcgcg cacgctcgtg cggtgcgcgc aggtcgcgcc cgcgctggaa
     7021 cgcaccctcg agctgccgaa cgacgaggcc gatgcccggt tgcgctcgat cgccggcatc
     7081 ggcgtgtgga cgtcggccga ggtgcggttt cgcgcccacg gtgacgccga cgcggtcagc
     7141 ttcggcgact accacgtgcc caatgagatc gggcacgccc tgctcggtca cgacctggac
     7201 gatgccggcc tggcggagtt gctcgagccg taccgtccgc atcggcaccg ggtgcaggcg
     7261 ctgctcggca cccggcgctg gcgcgagcgg cacgggccac ggatggcacc gcgcacccac
     7321 ctgccggcac gctgatggcg tcggttcgcc acagcggtca ttcgcagcaa acccggcgca
     7381 ggcaccgccg gtcccgctga tcgcgtcagt tcgtcacagc ggtcacccgc agcaagaccg
     7441 gcgcacccac ctgccggccc gctgacccgc tgccgccctc acccgatgcg gcgcacctct
     7501 tgcggccagc cgcagcccac ggcgatcttg cccgcccaat gtttggcggc ctcgtcgtcg
     7561 gccaccgaga cgatggtgaa cccgccgagg cgctggggcg tgtcggtgta cgggccgtcg
     7621 gtgacgacca cctcaccgct ggtggcgtcg gcggcgtaga ccgggccgtt gacctcgaca
     7681 ccggcggcgt acacgtacac ccccgcggcc ttcatctcgt cgaccaccgc attcgagggc
     7741 ttctcgcgcg agcggaacca gtcctcgctg tgatcaccga cccactgctg gttgaaatag
     7801 atcaggtagt cgggcatgtg tgctcctcct cgtcatggcc tgctccctgc ccgggttggc
     7861 tgcaccctac gagcctgtgc cgacaaaaac gagggtgaag ccgcccggct cgaccaccac
     7921 ccgtccaccg cgggtcgcct gacgggtgcg catgccgttg cggtcgtacg actcaacctg
     7981 cagccgcccg cccggcagcg acaccgtccg cagctgacgt cgctcactct tcgaggtcag
     8041 caacaccacc gctcccgtcg acccggacgc ctccagccgc gcgatctcgg gcatcaccag
     8101 cagcgcgtcc agccgggcgg agcccgcgcc ctgcacggtg gcggtgaccg tggtgccggc
     8161 gctcgcgaga ccgtccaggg cgaccggtgt gagccgtagc ggtgaggccg catcaccctg
     8221 cgggccgacg tcgccgaagc gcacccggcc gagccggcga tcgcccgcgg tgaaccgcac
     8281 ggacgccgcc gacccgtgct cgagtgccac caccggctgg acgagcagcg ccccggccga
     8341 ggcgggcagc gtccaggtga tcgtcgcgcc gtcgccgccg accacccgct tgccgctcca
     8401 cagcgactca ccggtccagg cggggtcgga ggactcgacc ctcgcactgc cacccagggt
     8461 gccggcttcg gcctcgatca cggtgagtcc gtcgcgcacc ccgatccgcg ccgaggcccg
     8521 ggccagccgg gccagtgcgg gggcggcgtc cagcacctgc atggtgagca ggccgtgaat
     8581 ggtcgactcg gcacccgagt tgcggttgag ccggccgtcg gcctcgacgc cgtcgaaggt
     8641 gaccccggtg gcggggtcgt aggtcggcac gccggcccgg ttcgcgccga agaaccagcc
     8701 ggcggcgatg ccggccagcc gggtgatgcc ctcgcggccc gcgacccgtc cgacctcgta
     8761 gcaggcccgc atccgggcgt cggcgccgta ggcgatctgc accttctcga gcggcaccgg
     8821 cagccagccg ttgtcgggcc ccgtcgcggt gagcagctgc gcggtgaacc cggcggtgtc
     8881 gccgatcgca ggcctcagca ggccgggatc gcgcaacgcc ttggcggcgg cggccagccc
     8941 ctgcgccatc tcggatcccc acgcgtgcca gtcgtccaac gactcggccc agggcagcag
     9001 ggcacggaac ggccactgtg tggtgctgcc cgcagcgaac tcggcgaccc cgcgagccag
     9061 ccgccggcag gcccgccggg ccggcgccga gcccgtcgcg gcggcatagg gcgcgagccc
     9121 caggagcgcc tcggaggtgg catcggcgcc ctgcgtgatc aaccacgcgg gcaccctcac
     9181 cccgtgcagg tcgtggtagg tgccgtaacg gacgagggtg tcgcgctcga ccgctgcgat
     9241 cgccaactcc atccggcggc gcaggaacgc cgcgaacgcc ggatcgtgcc gggcgaaggc
     9301 gacgtagccc tcaccgaagg cccacagggc gcgggcggtc cagtacgact cgccggcgtc
     9361 ggccgggttg gggttgtcgg gcggggtcgg cgtggggttg agcgtgccgt ccggctgcat
     9421 ccagagcaga aactcgcccg cgtgctcgcc ggtcagggtc tgaaagtagg ccagcccgcg
     9481 cagttgctgg taggcctgcc gccgggcgtg ggacgaaccc gtcgcccgcc actcccgcag
     9541 gtagaccacc gcggcccggg cgatgtcgtc cgagtcgtag gcgccctgtc cccaggtgtc
     9601 ggacgccgcg tcgtaactgc cgccgcccac tggacggtaa ccgccatcgg cctgcacgtc
     9661 ggcgtagacc cagagcagcc cgacggccgg ctcctggggc agccggtagg tggagtgcgc
     9721 agcggtgtga cgcagccgga cggtggtggt cagctcgtcc agatgggaca ggttggtcag
     9781 tcgcgtggct gctcgggcgg acggcgcggc gcccagtccg gtgagcacgg cgcctgctcc
     9841 ggcggcggcc agcagcgtgc ggcggggaat gcggggtggt tgcgtcatga cgaactcctt
     9901 cggggtgtgg gggatgcgcg ttcgatcagc ggtgtgggtg cgtgcgatca gcggtgtggg
     9961 ggcgcgttcg atcagcggtc tgggggcgcg tgttcgacca gtggtgtggg gtgcacgttc
    10021 gatcaggggc cggggtcaat ccacccgctc gatgcgggcc actccgatcg cctcgtcggc
    10081 catgccgtag aaggtgaaca gggcccgctc gatctcgacg atcgcggtgg ggaagaccac
    10141 gttggccacg atgccgaccc gctcgtccgc ggtctcggcg ctgagcaggg gactggtcgt
    10201 gcgggcgatc acccgcgacg ggtcgtccgc atcgagcagc aggccaccgg cggcgtagtg
    10261 cacgttcttc tgcggctcga agggattgct cgacatggtg cccgacacgc cgtgatagag
    10321 cagcagccac ccctgcggca cccggacggg cggtggcccg ccaccgatct tcaggtgctc
    10381 ccagtcggcc tgcgagcggg cgagttcgcg gtgcccgaac ggacgcagca gcgcgtccgg
    10441 gtcggcgatc gcgtccgcga gccgcacgta cgacaaccag atcgccgcgc gggcgtcggt
    10501 gctgccggcc ggcagctcga cctgctggcc gggcaactcg aacatcggac ggtgcagaaa
    10561 ccccaggcac gcgctgccgt ccgggccggt gaccggatcg ggaaagaacg ccaggtcctt
    10621 gttggcgaac aggttgagat cgacaccgag gtcgtcgtcg taccggaact ggacggggcc
    10681 gagccgcgtc cagtcgatca ggtcggggga ggtggccagc gccgcccgcg gcccggctgg
    10741 tccgtaggcg acgtaggcca tcaggtagag ccccaacgcc gacacgaacg tgatgcgtgg
    10801 gtcttcgacg ccgccgtgat ccgagccccg ctcgaaggcc cggtcggggg caagtgcgat
    10861 gccgcgccgc tgcacaccga tcggcacgcc gtcgccgatc accacgtcgg cgacggcgat
    10921 ccgggagaag ttgccgcggg ccaccacgcg cggaaacagc ttcagccgcc cgtccggagc
    10981 ccaggcggcg gccgggttga gcacaccctc ggcctcgcgg gcatcgttcg ggtcgggccg
    11041 catcaccacg cctgcgcggg tgagccggta gggaaaatcg gtcatggcgc gcatggccct
    11101 tcgtcggcga acatctcagc ccttcaccgc gccgccgacc acgctggcga ccaggtagcg
    11161 ctgcaggaac aggtagacca gcagcaacgg cgccgccacg atcatggtgg ccgccgacag
    11221 cagcgaccat tcgctggtgt aggtgccctt gaagttgaac aggcccaggg tgaccggcca
    11281 gtgttcctga ctgctgaaca ggatcgtggc cagaatgatg tcgttccaca ccccgatcgc
    11341 ctgcagaatg aacgccgtcg acagcaccgg catcatcagc ttgggcaaga aggtgaacag
    11401 ataacgcagg tagccgcagc cgtccatcgc cgccgcttcg tcgatctcgc gcggaatcga
    11461 gttcaggtag cccgcgatca gcaacggccc gacgccgacg ccagaggcca tgatcagtat
    11521 gtagcccagc ggcgtgtcgt acaggttcag ccgcagggcc agctggaaca ccgtcaccag
    11581 ggtgttcggc agaaacagca tcgaggcgaa cagccccatc cagaccttcg ggtgtttcac
    11641 gtagccgcgg gccaccggca ccgcgatcat cagcgacatc acggtgccca gcgcggcggc
    11701 cacgaacgtg tagagcaccg agttgagcat gtagctggca aagttgccct gctgccaggc
    11761 ggcggcgaag ttgccgaact gcggcgactg caccacgccg gtcgggttga ccagaaactc
    11821 gttgttgctc ttcagcgcgg tgttcaccag gaacagcagc gggtagaggt agatcacgat
    11881 caccagggcc atcagcgcgt acgacgccac ggccaggccg tggttgcgcg gcgcacccac
    11941 cgattcggcg gcgggacctc gtccggacgg ggcggtgctc gcgcccgccg gtacggcggc
    12001 gaccggcatg gtgctcacag cgcggtctcc cttctgcgca ggtaggccag ggtggccagc
    12061 gagatgatcg ccacgatgat gaactgcacc accgcgatag cggcggcgta cccctgcgac
    12121 tggacggcgc cgccctgcgg cccgccgaag ccctgggtga agatggccag gctgagcacc
    12181 tgggtcgcgg ggttgatcgg cccggtcagc acgtagatca gctggtagct ctgcagggcc
    12241 ccgatgatgc agagcagggt gttggcggtg acggacggcg ccaacagcgg gatcgtcacg
    12301 tgccgcagct tctgtcctcc ggtggcgccg tcgatgtcgg ccacctcgta caactcctgc
    12361 ggaatcgcct gcaggccggc caggtagatc accaccgcga ccccgatgcc gctccacacc
    12421 tgcaccagga tcaccagcgt catggccagg gacggatcgc cgaagaaggc gctggacgcc
    12481 ccgaacatgc cccatagctg cgcggccggg cccgcggacg ggttgaagat cagcgagaag
    12541 atcaggccga tcacggtgac gcccagaatg gtcggcagaa acaccaccgc ccgggcgaag
    12601 ttgcggccct tgagtcgctg gttcagcagc accgcgatca gcagggcgat cacgttttga
    12661 aagatggtca ccgcgaaggc gaacacgaac gtgttctgca gtgccgccac gttgtaggcg
    12721 agctgggcgg aactgaagaa cgtccgatag ttgtcgagcc ccacccagcg caccggcaga
    12781 aacggcagcg agcgaatgtc ggtgaacgag atcaccacaa tgccgacggc cggaatcagc
    12841 gagaacacca cgatcaacgc catcccgatg gcgaaggtca actgggcggt ccagcgccgt
    12901 ccgaagacga tgtagctgtt gctcacaacg cgctcctgat cgtgtgggtg aaaggccgga
    12961 tcggacgcgt accgtcatcc gacccggcca cccgggggag gaaactactt gccggcttcc
    13021 cagtccttct gggacgcatc ggccgcggcc tgggcgtcca tcgatcccat cggcttgacc
    13081 gcgctccagt tgaacggaag ggccgccgcc tggccggcct tggtgttggc gatccagatg
    13141 gtgtcccagg cgggctcgaa ctgcttggtg tacgtggcga tctcggcgta gaacgggtcg
    13201 ccctcgacac cctcctgcga gggcgcgaac ccggcctcgt cgttgaacag cttgtagttg
    13261 tcggcgaaga aggccagcca cgccatggcg gcgtccttgt tcttggcgtt gctcggcacc
    13321 gccaggctca actcgacctt gccgcccagg taggcgttgt cggcggcatt gtcgctggcc
    13381 ggcagcggaa agtacccgaa gtcgaccttg tcggcctcgc gcagcgaacc cacgttccag
    13441 gtgccgtccg agatcatcgc ggccttgccg gtgagaaact ccgaggtcat ctggttgtag
    13501 ttcgagccgg cgaagttcgg ctggccgtag gtgtagatct gctgcacctt cttgagcacc
    13561 tcaagctgct ggccctggtt cagcgcgacg gtgtgttcgt agaggccctt ggcgaggtcg
    13621 tccttgttct gcgcggtcgg gtacaggctc tggacgacgc tcagcatgac gatgcccgcg
    13681 gtgtctttgc cgccgatcga gatgggtgtg atgcccttgg tcttcaactg gtcgagcacc
    13741 ttgatgaagt cgctccaggt ggtcggaacc gtgatgttgt tgtcggcgaa gatcttcttg
    13801 ttgtaggtca ggccggtgta gtacgacagg cccgtcggca cggtgtagtt gcggccgttg
    13861 tacgtgatct tgtcgagcac cgaggcgttg aacttcttca tgaacgcctg gtcggtcagg
    13921 tcgacgaacc cgcccgcttc ggccagccgg gcgtcatcgc ccttgttgct ctcgggcacg
    13981 tagctgggca actgacgcgg gttggccagg ccgacgtcga tggtgccggc gcgggtgtcc
    14041 aggtcccggt gccggtagcc gt
//
LOCUS       DAOSUG010000010        13832 bp    DNA     linear   ENV 10-JUN-2024
DEFINITION  MAG TPA_asm: Micropruina sp. isolate AMR_MDS_2195
            NODE_2656_length_13832_cov_3.945199, whole genome shotgun sequence.
ACCESSION   DAOSUG010000010 DAOSUG010000000
VERSION     DAOSUG010000010.1
DBLINK      BioProject: PRJNA925477
            BioSample: SAMN32864262
            Sequence Read Archive: SRR4236673
KEYWORDS    WGS; ENV; Metagenome Assembled Genome; MAG; Third Party Data; TPA;
            TPA:assembly.
SOURCE      Micropruina sp. (wastewater metagenome)
  ORGANISM  Micropruina sp.
            Bacteria; Bacillati; Actinomycetota; Actinomycetes;
            Propionibacteriales; Nocardioidaceae; Micropruina.
REFERENCE   1  (bases 1 to 13832)
  AUTHORS   Abdulkadir,N., Saraiva,J.P., Zhang,J., Stolte,S., Gillor,O.,
            Harms,H. and Rocha,U.
  TITLE     Genome-centric analyses of 165 metagenomes show that mobile genetic
            elements are crucial for the transmission of antimicrobial
            resistance genes to pathogens in activated sludge and wastewater
  JOURNAL   Microbiol Spectr 12 (3), e0291823 (2024)
   PUBMED   38289113
REFERENCE   2  (bases 1 to 13832)
  AUTHORS   Abdulkadir,N.
  TITLE     Direct Submission
  JOURNAL   Submitted (24-MAR-2023) Environmental Microbiology, Helmholtz
            Center for Environmental Research -UFZ, Permoserstr 15, Leipzig
            04318, Germany
COMMENT     The annotation was added by the NCBI Prokaryotic Genome Annotation
            Pipeline (PGAP). Information about PGAP can be found here:
            https://www.ncbi.nlm.nih.gov/genome/annotation_prok/
            
            ##Genome-Assembly-Data-START##
            Assembly Method        :: SPAdes v. 3.11.1
            Genome Representation  :: Full
            Expected Final Version :: Yes
            Genome Coverage        :: Not Applicable
            Sequencing Technology  :: Illumina MiSeq
            ##Genome-Assembly-Data-END##
            
            ##Genome-Annotation-Data-START##
            Annotation Provider               :: NCBI
            Annotation Date                   :: 05/04/2023 04:57:10
            Annotation Pipeline               :: NCBI Prokaryotic Genome
                                                 Annotation Pipeline (PGAP)
            Annotation Method                 :: Best-placed reference protein
                                                 set; GeneMarkS-2+
            Annotation Software revision      :: 6.5
            Features Annotated                :: Gene; CDS; rRNA; tRNA; ncRNA
            Genes (total)                     :: 2,776
            CDSs (total)                      :: 2,750
            Genes (coding)                    :: 2,714
            CDSs (with protein)               :: 2,714
            Genes (RNA)                       :: 26
            tRNAs                             :: 24
            ncRNAs                            :: 2
            Pseudo Genes (total)              :: 36
            CDSs (without protein)            :: 36
            Pseudo Genes (ambiguous residues) :: 0 of 36
            Pseudo Genes (frameshifted)       :: 2 of 36
            Pseudo Genes (incomplete)         :: 32 of 36
            Pseudo Genes (internal stop)      :: 2 of 36
            ##Genome-Annotation-Data-END##
FEATURES             Location/Qualifiers
     source          1..13832
                     /organism="Micropruina sp."
                     /mol_type="genomic DNA"
                     /submitter_seqid="NODE_2656_length_13832_cov_3.945199"
                     /isolate="AMR_MDS_2195"
                     /isolation_source="Activated sludge of municipal
                     wastewater treatment plants"
                     /db_xref="taxon:2737536"
                     /environmental_sample
                     /geo_loc_name="Canada"
                     /lat_lon="49.25 N 123.1 W"
                     /collection_date="2015-06-16"
                     /metagenome_source="wastewater metagenome"
                     /note="metagenomic"
     gene            <1..281
                     /locus_tag="PLF56_00670"
     CDS             <1..281
                     /locus_tag="PLF56_00670"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_004808575.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=3
                     /transl_table=11
                     /product="fumarate reductase/succinate dehydrogenase
                     flavoprotein subunit"
                     /protein_id="HOQ52104.1"
                     /translation="GVNEDFNQALERAGRVADFFELGELMCIDALHRRESCGGHFRAE
                     SQTPDGEALRHDDEFLYVGAWEFAGDTPVLHKEPLEYEFIELKQRSYK"
     gene            278..1039
                     /locus_tag="PLF56_00675"
     CDS             278..1039
                     /locus_tag="PLF56_00675"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_014846372.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="succinate dehydrogenase/fumarate reductase
                     iron-sulfur subunit"
                     /protein_id="HOQ52105.1"
                     /translation="MNITLRVWRQAHGATNGGMQTYQLQDVSPDMSFLEMLDLLNEKL
                     TVEGIEPVAFDHDCREGICGMCGVVINGSAHGRGASPVPTTTCQLHMRSFADGATIDV
                     EPWRAGGFPVIKDLVVDRTAFDRIIQAGGFISVNTGSAPDANAAPVSKVDADRAFDAA
                     TCIGCGACVAACPNGSAMLFTSAKITHLAMLPQGQPERWSRVKGMVAQHDAEGFGNCT
                     NIGECAAVCPKEIPLDTISQLNRELMHALFKKDAK"
     gene            1534..1884
                     /gene="rplS"
                     /locus_tag="PLF56_00680"
     CDS             1534..1884
                     /gene="rplS"
                     /locus_tag="PLF56_00680"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_015070816.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_component: GO:0000311 - plastid large ribosomal subunit
                     [Evidence IEA];
                     GO_component: GO:0022625 - cytosolic large ribosomal
                     subunit [Evidence IEA];
                     GO_function: GO:0003735 - structural constituent of
                     ribosome [Evidence IEA];
                     GO_process: GO:0006412 - translation [Evidence IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="50S ribosomal protein L19"
                     /protein_id="HOQ52106.1"
                     /translation="MNKIVAEVTQASLRSDIPDFRPGDSVRVHVKVVEGNRSRIQVFA
                     GVVIARNGSGIGEAFTVRKVSFGTGVERTFPLHSPIIDKIEVERHGDVRRAKLYYLRG
                     LRGKAAKIKEKRDR"
     gene            1926..2678
                     /gene="lepB"
                     /locus_tag="PLF56_00685"
     CDS             1926..2678
                     /gene="lepB"
                     /locus_tag="PLF56_00685"
                     /EC_number="3.4.21.89"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_020389438.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_function: GO:0004252 - serine-type endopeptidase
                     activity [Evidence IEA];
                     GO_process: GO:0006465 - signal peptide processing
                     [Evidence IEA];
                     GO_process: GO:0009306 - protein secretion [Evidence IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="signal peptidase I"
                     /protein_id="HOQ52107.1"
                     /translation="MSETPTSGKPSAGRVAGAWAKEIAIVVVGALIASTLLRLFVAQM
                     FVIPSGSMENTLQIRDRVMVQKVAGWSRGDIVVFRDTQGWLADPAEETDPVRKALIFI
                     GLAPDESSNHLIKRVIGLPGDHVKCCDAQGRITVNGQPLDESGYLFSENGETVAPSDF
                     PFDVIVPKDRIWVMGDHRNNSQDSRCHLSEGTGGIAAFVPTANVVGSAVAVVYPFDRW
                     RTLPRPSTFDQVPAASGPAPDTPVITGPIPNC"
     gene            2685..3344
                     /locus_tag="PLF56_00690"
     CDS             2685..3344
                     /locus_tag="PLF56_00690"
                     /EC_number="3.1.26.4"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_002548297.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="ribonuclease HII"
                     /protein_id="HOQ52108.1"
                     /translation="MPFVLRRDAGLYGYERALRRAGFALIAGADEAGRGACAGPLVAA
                     SVILGSTPGSHVQGLKDSKLLTPLQRERCYDEIVAKAIAWSVVLIDPGECDRLGMHVS
                     NIQALRRAVCRLDVRPDFALTDGFGVDGLGVPSLAVWKGDAVAGCVAAASVIAKVTRD
                     RLMCAAHERWPQYRFDVHKGYCTPLHQQHLTANGPCEIHRWCFENVRRAAGVPDAPPA
                     G"
     gene            3562..3873
                     /locus_tag="PLF56_00695"
     CDS             3562..3873
                     /locus_tag="PLF56_00695"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_016455603.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="DUF2469 domain-containing protein"
                     /protein_id="HOQ52109.1"
                     /translation="MSAEDLEQYESDLELQLYREYKDVVGIFTYAVETERRFYLCNAV
                     DLKVRTEGGDVYYEVSMGDAWVWDMYRPARFVKSAKVLTFRDVSIEEIAHADLKVPEN
                     L"
     gene            complement(3976..4884)
                     /locus_tag="PLF56_00700"
     CDS             complement(3976..4884)
                     /locus_tag="PLF56_00700"
                     /inference="COORDINATES: protein motif:HMM:NF015431.2"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_function: GO:0001216 - DNA-binding transcription
                     activator activity [Evidence IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="LysR family transcriptional regulator"
                     /protein_id="HOQ52110.1"
                     /translation="MDPRRVLLFRAVARTGSLSAAARELATTQPAVSQQLRALEREAG
                     MPLVLRGARGITLTEAGQRLLARADGIAAHLHMASEELAGLAHLRAGRVRLVAFPSAA
                     ATLVPDALAALADDHPGVAVEMVEAEPPEAAAAIRQGEADVAVVFSYPDAGVGDERLR
                     APEDAGDLAWVPLGDEAVQLVVASGAHERPSDLQHETWIAGCPRCRGHLVTCCRRAGF
                     EPRISFESDDYVVVQNLVARGLGITALPALAMRAFLHPGVTVSDSELFGRRTVGALHR
                     EGAEQVSATRALLERLQRSAGKVLAG"
     gene            4961..5659
                     /locus_tag="PLF56_00705"
     CDS             4961..5659
                     /locus_tag="PLF56_00705"
                     /EC_number="5.1.1.-"
                     /inference="COORDINATES: protein motif:HMM:TIGR00035.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_function: GO:0047689 - aspartate racemase activity
                     [Evidence IEA];
                     GO_process: GO:0006520 - amino acid metabolic process
                     [Evidence IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="amino acid racemase"
                     /protein_id="HOQ52111.1"
                     /translation="MRTIGLIGGMSWHSTMTYYRLLNESVARELGGHSSARIVLQSLH
                     FAEIRECQLSGDWSRAERILTEAALACQGGGADVVAICTNLMHKVAPGVEASLNVPLL
                     HIADAVAAEADRRDWTRLGILGAGWTMREDFYATRLARHGKTAVVPGADDCDLVDRVI
                     FDELTRGVVREESRRAFLRVIERLAAAGAQAVVLACTEIGLLLEPGTARLPMIDSAEV
                     HANELGRLAVAVTA"
     gene            5739..6104
                     /locus_tag="PLF56_00710"
     CDS             5739..6104
                     /locus_tag="PLF56_00710"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_012922321.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_function: GO:0003676 - nucleic acid binding [Evidence
                     IEA];
                     GO_function: GO:0004518 - nuclease activity [Evidence
                     IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="YraN family protein"
                     /protein_id="HOQ52112.1"
                     /translation="MEKSAVGRAGEVAAVQHLVTRGYTIVERNWRCSLGEVDIIAQTP
                     GAEPVLVFCEVKCRTGLGYGDPLEAITWRKLARLRQLALTWLGEQPGWVPRYRVDAIG
                     VLLPRSGRPQVTHVEGVGA"
     gene            6101..7645
                     /locus_tag="PLF56_00715"
     CDS             6101..7645
                     /locus_tag="PLF56_00715"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_013862565.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_function: GO:0005524 - ATP binding [Evidence IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="YifB family Mg chelatase-like AAA ATPase"
                     /protein_id="HOQ52113.1"
                     /translation="MSTARAFSVALVGVEGAIVEVEAALGGGLPRMVIVGLADAALGE
                     ARERCRAAVHSQGLGWPTQLVTVNLSPATLPKAGSHFDLAIVTSVCAAADRFDSAVLD
                     GMVLLGELGLDGRVRSVRGLLPALLAAVASGFHTAVVPACQLREARLVDGLTIWGVDD
                     LLDLVAVLVGDPPPQVVRAPQQAVDTTEPVKDLSDVMGQLEAKWALEVAAAGRHHVFL
                     HGPPGVGKTLLAERLPGLLPDLGQSEALEVSAIHSLAGLPLSDGLVRRPPYADPHHSA
                     SLASIVGGGARMALPGSISRAHRGVLFLDEAPEFSPRVLEALRVPLESGRVVLGRAMV
                     HAVYPASFQLVLAANPCPCGNAGTRGAECRCAPMAVRRYAERISGPILDRVDIHHHLA
                     PMSRTYLKAAKATGESSAAVAERVGEARARQQQRLAEHGWLTNGEVPGPALRRLLPLP
                     KGVDLLDEAVSRGRLSARGVDKVIRLSWTIADLAGADLPTRDHLHTALAMRRGEQLGE
                     VTRARA"
     gene            7632..8762
                     /gene="dprA"
                     /locus_tag="PLF56_00720"
     CDS             7632..8762
                     /gene="dprA"
                     /locus_tag="PLF56_00720"
                     /inference="COORDINATES: protein motif:HMM:TIGR00732.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_process: GO:0009294 - DNA-mediated transformation
                     [Evidence IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="DNA-processing protein DprA"
                     /protein_id="HOQ52114.1"
                     /translation="MHEREARMALCCAVEPGSRELAALVDRHGAEAVWRSLLKPGSPS
                     RWAARAQRLDLAAVGRATERFRLRFVIPGDDDWPGGLSDLADCEPVQDLGGAPFGLWV
                     KGQMALVEAVARSVSIVGARACTAYGQTVTTDLAAGLGTHGITVISGGAYGIDAAAHT
                     GALAADAPTVAVMAGGLDKPYPSGNSALLQRVANHGLLVSELPPGEHPTRVRFLARNR
                     LIAALTPATILVEAALRSGARNTVSWASACRRLVLAVPGPVYSATSATPHRLIREAEA
                     VLCAGVEDVLELVGPLGRRPAHRPSQHRTLDDLSPDELAVFEALPARGLVASGEVSLK
                     AGVPLGSCLGVLDRLAERGLVAQGVDGWRLAKASFGASVEGA"
     gene            10037..10294
                     /locus_tag="PLF56_00725"
     CDS             10037..10294
                     /locus_tag="PLF56_00725"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_013864459.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="GlsB/YeaQ/YmgE family stress response membrane
                     protein"
                     /protein_id="HOQ52115.1"
                     /translation="MLIIGIILFGMVVGAGAQLILGKKPVNWPMAFASGLAGSFVGGL
                     LISLLSGDGLELRPSGLIGSLVGAVIVTGVWQWWQSRSATT"
     gene            10328..11476
                     /locus_tag="PLF56_00730"
     CDS             10328..11476
                     /locus_tag="PLF56_00730"
                     /EC_number="2.6.1.-"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_007079464.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_function: GO:0003824 - catalytic activity [Evidence
                     IEA];
                     GO_function: GO:0030170 - pyridoxal phosphate binding
                     [Evidence IEA];
                     GO_process: GO:0009058 - biosynthetic process [Evidence
                     IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="pyridoxal phosphate-dependent aminotransferase"
                     /protein_id="HOQ52116.1"
                     /translation="MNRALSGFTSTIFAEMSALATATGSVNLGQGFPDYDGPAGMLER
                     AQRAIAEGVNQYPPGPGTLELRTAIAAHQQRWYGVALDPEREVLVTAGATEGIAAAML
                     GLLEPGDEVLLLEPYYDSYAATIALAGAVRRPVRLEAPDFALPRERLAAAVGPRTRLL
                     LLNSPHNPTGMVLSADDLAFVASLAVRHDLIVVTDEVYEHLIFDGVSHVPLCTLPGMF
                     DRTLTISSAGKAFSVTGWKIGWVSGPAELVAAVRAAKQFLTYVNGAPFQPAVAEALGL
                     DDAFFTTQRRALQAKRDRLTDGLRTAGFAVTRPSGTYFVVAEGRPLGFDDGYALCREL
                     PELCGVVAVPLSVFYDDADGVGSLVRFAFCKRDEVLDEAVTRLARLAG"
     gene            complement(12043..12879)
                     /locus_tag="PLF56_00735"
     CDS             complement(12043..12879)
                     /locus_tag="PLF56_00735"
                     /EC_number="3.1.3.-"
                     /inference="COORDINATES: protein motif:HMM:NF019887.2"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_function: GO:0016787 - hydrolase activity [Evidence
                     IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="HAD family hydrolase"
                     /protein_id="HOQ52117.1"
                     /translation="MGKTVFLDFDGTYAHNGVVPPAHVRAVREAQRNGHRVLLCTGRP
                     KALVTPEDVEVFDGLVAAAGGYVEVEGEVLADRRFSSELAARTITLLDANEAVYILEG
                     PDAAYVGPGMKQRLVALLTGAEHGPSFGEAADGILDPLRVSDDPASESFAKITCFDCA
                     VPTSWIGAQLAPEVAVVPSSIGSLGERGGEFFLSDVNKSVGIAVVEQHFGIDRDDIIA
                     IGDSHNDLEMVAYAGTGVAIEGGVPELLAGADLIVPPPERDGLVVAFERLGLIDTSAR
                     AA"
     gene            complement(13029..13706)
                     /locus_tag="PLF56_00740"
     CDS             complement(13029..13706)
                     /locus_tag="PLF56_00740"
                     /inference="COORDINATES: protein motif:HMM:NF013079.2"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="M17 family metallopeptidase"
                     /protein_id="HOQ52118.1"
                     /translation="MSLKPSDPMHLLMKIDMGGAAAVLGAFTALRDLGATAAVTGWLC
                     CTDNMPSGSSYKLGDVLTCRNGTTVEVKNTDAEGRLAMMDGLALAVEAKVDAIVDIAT
                     LTGAALMALGQSRAAILGNDQAMVDRVSAAAAATDEPVWQLPLERKYRKQLDSEIADI
                     ANLGGRFAGCTTAALFLEEFVDGTPWAHIDIAGTMSTDADDSWRSSGATGYGARLLAS
                     LAADYSA"
BASE COUNT         2027 a         4673 c         4849 g         2283 t
ORIGIN      
        1 ccggcgtcaa cgaagatttc aaccaggcct tggagcgggc cggccgggtg gccgacttct
       61 tcgagttggg cgagttgatg tgcatcgacg ccctgcatcg ccgcgaatcg tgcggcgggc
      121 acttccgggc cgagtcgcag acccccgatg gcgaggcgct tcgtcacgac gacgagttcc
      181 tctacgtcgg agcctgggag ttcgccggcg acacaccggt gctgcacaag gaacccctgg
      241 agtacgagtt catcgagctg aagcaacgga gttacaagtg aacatcactt tgcgtgtgtg
      301 gcgtcaggcc catggcgcga ccaacggcgg catgcagacc taccagctgc aagacgtcag
      361 ccccgacatg tcgtttctcg agatgctcga cctgctgaac gagaagctga ccgtcgaggg
      421 catcgagccg gtcgcgttcg accatgactg tcgcgagggc atctgtggca tgtgcggagt
      481 ggtgatcaac ggttcggcgc acggacgggg cgcgtccccg gtgccgacca ccacctgcca
      541 gctgcacatg cgttcgttcg ccgacggggc cacgattgac gtggagccct ggcgggccgg
      601 cggcttcccg gtgatcaagg acctggtggt cgatcgcact gccttcgacc gcatcattca
      661 ggcgggcggc ttcatctcgg tcaacaccgg ctcggcgccg gacgccaacg cggcgccggt
      721 gtcgaaggtg gacgccgacc gggccttcga cgcggccacc tgcatcggct gcggtgcctg
      781 tgtggcggcc tgcccgaacg gttcggcgat gctgttcacc tcggccaaga tcacccatct
      841 ggcgatgctt ccgcagggcc agccggaacg ctggtcgcgg gtcaagggca tggtggcgca
      901 gcacgacgcc gagggcttcg gtaactgcac caacatcggt gagtgcgcgg cggtctgccc
      961 caaggagatt ccgctcgaca cgatcagcca gctcaaccgc gagttgatgc atgcgctgtt
     1021 caagaaggac gccaagtagg cattcgacga aggtccccgg ggccctggtg ggtcgcgggg
     1081 accttcgccc tgctcaggct cgtccggatt cgttggcgac cgttctcgcg cactttctgg
     1141 ggacggttct cacaccggtg ctggtcgccc tgacgcgtgt gtcgcggaat cgggtttcac
     1201 caccaccccg cgcgacgccg cgggccggtg ctgacaggcg tattccgggc cggcgaggag
     1261 cagaacgtcg cggcaagggt tgggagagcg gcctccgagc cgagctgaga aacgtccccg
     1321 gaccagacga gcgaagttcc cggccagtga tgtcgtgtcc ccgactcagc cagcgtgcac
     1381 ccgtactacc cccgcacgat tacgggtcgg acgagtggat gtggcagact tgatccttgg
     1441 tgccggacgc ccctgccaca gggggaaggt gtccacccgc atcacccatt gatccaccac
     1501 gaggtgtcct gtggcaccgg cgaggaaatc cccatgaaca agatcgttgc cgaggtcacg
     1561 caggcctccc tccgctccga cattcccgac tttcgtccgg gcgactccgt gcgcgtgcac
     1621 gtgaaggtcg tcgagggcaa ccgctcccgc atccaggtgt tcgccggcgt ggtgatcgct
     1681 cgcaacggca gcggcatcgg tgaggcgttc acggtgcgca aggtcagttt cggcaccggc
     1741 gtcgagcgca cgttcccgct gcactccccg atcatcgaca agatcgaggt cgagcgccac
     1801 ggtgacgtcc gccgcgcgaa gctgtactac ctgcgtgggc tgcgcggcaa ggctgccaag
     1861 atcaaggaga agcgggaccg ctgatcctct gacccggctc cggtacttca ggcaggagac
     1921 ccgcggtgag cgaaacaccc acctccggca agccatcggc cggccgggtg gccggggcat
     1981 gggccaagga gatcgccatc gtggtggtcg gggccctgat cgcctcgacc ctgctgcggc
     2041 tgttcgtcgc gcagatgttc gtcattccgt ccgggtcgat ggagaacacc ctgcagatcc
     2101 gcgaccgggt gatggtgcag aaggtggccg gctggtcgcg tggcgacatc gtcgtcttcc
     2161 gcgacaccca gggttggttg gctgaccccg ccgaagagac agatccggtc cgcaaggcgc
     2221 tgatcttcat cggcctggcg cccgatgaaa gctcgaacca tctgatcaag cgtgtgatcg
     2281 gcctgcccgg cgaccatgtg aaatgctgcg atgcgcaggg gcgcatcacc gtcaacggcc
     2341 aacccctgga cgagtccggg tatctgttct ccgagaacgg cgagacggtc gcgccgtcgg
     2401 attttccctt cgacgtgatc gtgcccaagg atcgcatctg ggtgatgggc gaccaccgca
     2461 acaactccca ggattcccgc tgtcacctct ccgaaggcac cggcggcatc gcggcattcg
     2521 tgccgaccgc caacgtggtg ggttcggccg tcgcggtggt ctatccgttc gaccgttggc
     2581 gaaccctgcc ccgtccctcg acgttcgacc aggttcccgc tgcgagcggc cccgcccccg
     2641 acaccccggt gatcaccggt ccgatcccga actgctgagg tgccatgccc ttcgtgttgc
     2701 ggcgggacgc gggactgtac ggctatgagc gggctctgcg tcgggccggc ttcgcgctga
     2761 tcgcaggtgc tgacgaggcc ggacgggggg cgtgtgccgg ccccctggtg gccgcgtcgg
     2821 tgattctggg cagcacgccc ggttcacacg tgcagggcct gaaggattcg aaactgctca
     2881 cgcctttgca gcgtgaacgc tgctacgacg agatcgtcgc caaggcgatc gcctggtcgg
     2941 tggtgttgat cgatcctggc gagtgcgacc ggctgggcat gcacgtgtcg aacatccaag
     3001 ccttgcggcg agcggtctgc cggttggatg tgcgccccga cttcgcgttg accgatggtt
     3061 tcggcgtgga cgggctgggc gtgccgtcgc ttgccgtctg gaagggggac gcggttgcag
     3121 gttgcgtggc ggccgcttcg gtgatcgcga aggtgacgcg cgaccgcctc atgtgtgcgg
     3181 cgcacgagcg ctggccgcag tatcgattcg acgtgcacaa ggggtattgc acgcccctgc
     3241 accagcagca tttgactgcc aacggcccct gcgagattca ccgctggtgc ttcgagaatg
     3301 tgcgccgggc ggccggtgtg ccggatgcgc ctccggcggg ctgagcggca acagagtttc
     3361 tgaaatcacg acaccagcgc gcacctcgac cggcgacacg ccgggtacgg gtgccactca
     3421 ggcgggtgat ttcagaccgc cccctcacga gcgccgccct gttgccccgg ttcgtggcgt
     3481 ttgttgcggg tgtccacgcc gaggctcgtg cgccagcccc tggcccgcgc gcccgacaac
     3541 cacgagtaga gtggccacgc tatgagcgcc gaagatcttg aacagtatga gagcgacctc
     3601 gaactccagc tctaccggga gtacaaggac gtggtcggca tcttcaccta tgcggtcgag
     3661 accgagcggc gcttctacct gtgcaacgcg gtcgacctga aggtgcgcac cgagggtggc
     3721 gacgtgtact acgaggtgtc gatgggcgac gcctgggtgt gggacatgta ccggccggcc
     3781 cgattcgtga agtcggccaa ggtgctgacg ttccgtgatg tcagcatcga agagatcgcc
     3841 cacgccgacc tcaaggtgcc agagaacctg tagagaaggc ctccgaccgc ctgtccccga
     3901 ttggttgggc ctcgctgggt tcacctgagc agatcgcgcg atgtgctgcg ctcgtgctga
     3961 gcgccgtcgg tgaggtcatc ccgcaaggac cttcccggcc gatcgctgca accgttcgag
     4021 cagcgcgcgg gtggcggaca cctgttcggc gccctcgcgg tgcagggcgc cgacggtgcg
     4081 gcgtccgaac agctctgagt cactcacagt cacgccgggg tgcaggaagg cccgcatcgc
     4141 caaggccgga agcgcggtga tgcccaggcc gcgcgcgacc aggttctgca ccaccacgta
     4201 gtcatcactc tcgaagctga tccgcggctc gaagccggca cgccggcagc aggtgaccag
     4261 gtggccgcgg cagcgtgggc acccggcgat ccacgtttca tgctgcaggt cggacgggcg
     4321 ctcgtgcgcg ccgctcgcca ccaccagctg caccgcctcg tcacccaggg gcacccacgc
     4381 gagatccccc gcgtcctcgg gggcgcgtag gcgctcgtcg ccgacgccgg cgtccggata
     4441 cgagaagacc accgccacgt ccgcctcacc ctgtcggatc gccgctgcgg cctcgggggg
     4501 ctcggcttcg accatctcga cggcgacgcc cgggtgatcg tcggcgagcg ccgcgagggc
     4561 gtccggaacc aacgtcgcgg cggcggacgg gaacgcgacc agccgaaccc gcccggcgcg
     4621 caggtgagcc aacccggcca gttcctcgct cgccatgtgc aggtgcgcgg cgatgccgtc
     4681 ggcccgggcg agcagccgct gacccgcctc ggtgagcgtg atgccgcggg cgccacgcag
     4741 caccaggggc atccctgcct cacgctcgag cgcgcgcagc tgctggctca ccgccggctg
     4801 ggtggtggcc aactcgcggg cagccgcgct gagcgacccg gtacgggcca cggcgcggaa
     4861 cagcagcact cggcgaggat ccattcccca agtctgtctt atgggtgctt caagtaacaa
     4921 aggtctacct atgaccctct gactccgcca cagttgccac atgagaacca tcggcctgat
     4981 cggcggcatg agctggcatt ccacgatgac ctactaccga ctactgaacg agtcggtggc
     5041 ccgcgagctg ggtggtcatt cgagcgcgcg catcgtgctg cagtcgctcc acttcgccga
     5101 gatccgcgag tgccagttga gcggtgactg gtcacgtgcc gaacggattc tgaccgaggc
     5161 cgcactcgcc tgccaggggg gtggggccga cgtggtcgcg atctgcacca acctcatgca
     5221 caaggtggcg ccgggggtgg aggcgtcgtt gaacgtgccg ctgctgcaca tcgcggacgc
     5281 cgtcgcggcc gaggccgacc ggcgagactg gacgcggctc ggcattctgg gcgccggctg
     5341 gaccatgcgt gaggatttct atgccacgcg cctggcccgg cacggcaaga cggcggtggt
     5401 gccgggtgcc gacgactgcg acctggtgga ccgggtgatc ttcgacgagt tgaccagggg
     5461 agtggtccgc gaggagtcca ggcgggcctt tctgcgggtg atcgagcgac tggcggctgc
     5521 cggggcgcag gcggtggtgc tggcttgcac cgagatcggc ctgctgctcg aacccggcac
     5581 ggcccggctg ccgatgatcg acagcgccga ggtgcacgcc aacgaactgg gacggcttgc
     5641 ggtggcggtc accgcgtgat cagtggccgt tgacgagtcc gcctggccgg ccgctgacac
     5701 tttgaacact tctcgactct cgggcacaag ctgaggacgt ggagaaatcg gcggtcggac
     5761 gggcgggcga ggtggcggcg gtgcagcacc tggtgacccg gggctacacc atcgttgagc
     5821 gcaactggcg ctgctcgctg ggcgaggtcg acatcatcgc gcagaccccg ggcgccgagc
     5881 ccgtgctggt gttctgcgag gtcaaatgtc gcaccgggct cggctacggc gacccgctcg
     5941 aggccatcac ctggcgcaag cttgcccggc tgcgtcagct cgcgctcacc tggctcggcg
     6001 agcagccggg ctgggtgccc cgctatcggg tggacgccat cggggtgctg ctgccccgca
     6061 gcggccgtcc acaggtcacg catgtcgagg gggtgggtgc atgagtacgg cacgcgcctt
     6121 ctcggtggcg ctggtggggg tcgagggtgc catcgtcgag gtcgaggcgg ccctcggcgg
     6181 cggtctgccg cgcatggtga tcgtcggtct ggccgacgcc gctctgggcg aggctcgcga
     6241 gcggtgccgg gcggcggtgc attcgcaggg gctcggctgg cccactcaac tggtcacggt
     6301 caatctgtcg cccgccacgc tgcccaaggc cggctcacac ttcgacctgg cgatcgtcac
     6361 gtcggtctgc gcggcggccg accgcttcga ctctgccgtg ctcgacggca tggtcctgct
     6421 gggtgagctg ggtctcgacg ggcgggtgcg ctcggtgcgg gggctgttgc cggcgctgct
     6481 cgcggccgtc gcgtccggct ttcacaccgc ggtggtcccg gcctgccagc tccgtgaggc
     6541 gcgtctggtc gacgggctga ccatctgggg cgtcgatgac ctgctcgatc tggtggcggt
     6601 gctcgtcggc gatccgccgc cccaggtcgt ccgggcacct cagcaggccg tcgataccac
     6661 cgagccggtg aaagatctca gtgacgtgat gggccagctc gaggccaaat gggcgctcga
     6721 ggtcgcggcc gcgggccgcc atcacgtgtt tctgcacggc ccgccgggcg tcggcaagac
     6781 cctgctcgcc gagcggctgc cggggctgct gcccgacctc ggtcagtccg aagcccttga
     6841 ggtgtcggcc attcactcgc tggcggggtt gccgctcagc gacggactgg ttcgccggcc
     6901 gccgtatgcc gatccgcacc actcggcctc actggccagc atcgtcggcg gcggtgctcg
     6961 catggcgctg ccgggcagca tcagccgggc ccatcgcggt gtgctgttcc tggatgaagc
     7021 gccagagttt tcgcccaggg tgctcgaggc cctgcgggtg ccgttggaat ccggacgggt
     7081 ggtgctcggc cgagcgatgg tgcatgcggt ctatccggcc agttttcagt tggtgctggc
     7141 cgccaacccc tgcccgtgcg gtaacgcagg cacccggggt gccgagtgtc gctgcgcacc
     7201 catggcggtg cgccgctatg ccgagcgcat ctcgggtccg atcctcgacc gcgtcgacat
     7261 tcatcatcac ctggcgccga tgagccgcac ctacctcaag gcggccaagg cgaccggcga
     7321 gagttccgcc gctgttgccg agcgcgtcgg tgaggcgcga gcgcgacaac agcagcggct
     7381 ggccgagcac gggtggctga ccaacggcga ggtgcccggc cccgccctgc ggcggctcct
     7441 gccgctgccg aagggtgtcg atctgctcga tgaggcggtc agccgtggac ggttgtcggc
     7501 acgcggtgtc gacaaggtga tcaggctctc gtggacgatc gccgacctgg ccggggccga
     7561 cctgcccact cgcgaccatc tgcacactgc cctggctatg cgacgcggcg agcagctggg
     7621 cgaggtgacc cgtgcacgag cgtgaggcgc gtatggcgtt gtgttgcgcg gtcgagccag
     7681 gtagccgcga gttggccgcg ctggtcgacc ggcacggggc cgaggcggtg tggcgatcgc
     7741 tgctgaagcc gggttcgccg tcgcggtggg cggcccgcgc gcaacgactc gatctggcgg
     7801 ccgtcggtcg tgccaccgaa cgcttccggc tgaggttcgt catacctggc gatgacgact
     7861 ggccgggcgg gctgagtgat ctggccgact gcgaaccggt gcaagacctg ggcggtgcgc
     7921 ccttcggctt gtgggtgaag ggccagatgg ccctggtcga ggccgtcgcg cgttcggtgt
     7981 cgatcgtcgg ggcccgggcg tgcacggcct atggccagac ggtgacgacc gacttggcgg
     8041 ccgggctggg cacgcacggc atcacggtca tcagtggggg cgcctacggc atcgacgcgg
     8101 ccgcgcacac cggtgcgctc gcggccgatg cgcccaccgt tgcggtcatg gcggggggcc
     8161 tcgacaagcc gtacccgagc ggtaacagcg cgctactgca gcgggtggcc aatcacggcc
     8221 tgctggtcag cgaactaccg cccggcgagc atcccaccag ggtgcggttc ctcgcccgca
     8281 accggctgat cgccgcactg acacccgcca ccattctggt cgaagcggcc ttgcgcagcg
     8341 gggcgcgcaa cacggtcagt tgggcgagcg cctgccgccg cctggtgctg gcggtgccgg
     8401 gcccggtgta cagcgccacc tcggccaccc cgcaccggtt gatccgtgaa gccgaggcgg
     8461 tgctgtgcgc cggcgtcgag gacgttctcg aactcgttgg cccgctcggc cgcaggccgg
     8521 cgcaccgtcc atcccagcac cggactctgg acgacctgtc gcccgacgaa ctcgccgttt
     8581 tcgaggcgct tcccgcacgc ggcctggtgg cctcgggtga ggtgtccctg aaagcgggcg
     8641 ttccgctggg cagttgcctc ggggtgctcg accggctcgc cgagcgggga ctggtcgccc
     8701 agggggtcga cggttggcgg ttggccaagg cgtcgttcgg ggcctcggtc gagggggcgt
     8761 gaaagcgagg ccggggcacc gcgtcgctgc gcatcccgcg ctgctcgagg tggccggcga
     8821 ggccttccgc gtcgtgcgtc tcggattgat tggtagcggg cgcggagaac tgcggcggtg
     8881 gcacacgcac cgatgccacc ggccactccc gccgggtgct ggtacacggc ttcgtggtgc
     8941 acccgccgtc ttcgtggtgc acccgccgtc ttcgtggtgc acccgccgtc ctcgtggtgc
     9001 acccgcccgt cggcagtggt ccacatgccc gtcgacgtgt gatcggggct cgccgagtca
     9061 ctcgcgcggg tgactgacac aacagcaatc gcggtctatc gtttctgatg tcggggggac
     9121 aagaagagac gctgatggcc gggtcgttca atactggggg gtacggaacg acccggccaa
     9181 cggcgtccca cgtagccatc acgcaacgtg cgatgggtgt gcactttgtg cgcgcagggc
     9241 ctgaggcccg aatgcgtgca gggtccagac atgtggatcc ccgtgacgtc accgtgccca
     9301 gcctcccagc gtgaaaggta cgagacccat gctggaatac cgccgaagct gtgagcgctg
     9361 cggccgcgag ttgtcaatcg atgaccacga ggtctacatc tgctccttcg aatgcacctg
     9421 gtgtgccgag tgcgtagccg gctttccacg cggtacctgt cccaattgcg ggggcaacct
     9481 cgcgctgcgg ccgatccgtc cacggcatct gacctccgcc gtgacgacgg ccaccaccac
     9541 ggggcagcat ccgcactccg actgccgtcg ctatctggcg cgaacggcct gagccaccac
     9601 gatccgttcc ggggactggc tgaccgggcc ctgggacagc cgactgtccc gccctgttcg
     9661 ctccgcgcgt gctgtgggtc agccctcagg tggcgagctt gggtcgtagc gtcgcgagga
     9721 tggtcgccgt agggcacccg cctgaagcgg acgaccgcca aacctaccgc ctcgcgcacg
     9781 tgtctcggac gtagccgcct tcgtggcagg tgactgcctc gcgcaggcag ttgcgcgtcc
     9841 ggaacgcagt cacgtcgggg gcgagggctt cactggatac gttgcccgct accagccggg
     9901 acgaacacgc ctggtgacag cgtcgccgag ccacacgagc gcaccaccag gtctcggggg
     9961 cgacccgtcg ctgccatgcg ggcacgccga cggccatgga tgcgtcgcac agcctgcggg
    10021 ccggtaagct tcctgggtgc tcatcattgg catcatcctg ttcggcatgg tcgtcggcgc
    10081 cggcgcccag ctcatcctcg gcaagaagcc ggtcaactgg ccgatggcgt tcgcgtccgg
    10141 gctggcggga tcgttcgtcg gtgggctgct gatcagcctg ttgtccggcg acggccttga
    10201 actgcgtccc tcgggtctga tcgggtcgct ggtgggcgcg gtgatcgtga ccggcgtgtg
    10261 gcagtggtgg cagtcccgct cggccaccac ctaggaattg tccgagcatc gcccgcatcg
    10321 tccggtgatg aatcgggcgc tcagcggctt cacctccacg atcttcgccg agatgtccgc
    10381 cttggcgacc gccaccggtt cggtcaatct ggggcagggt tttcccgact acgacgggcc
    10441 cgccggcatg ctcgagcgcg cgcagcgcgc catcgccgag ggagtcaacc agtacccgcc
    10501 gggcccgggc acgctcgaac ttcgcaccgc gatcgccgcg caccagcaac gctggtacgg
    10561 cgtcgcgctc gaccccgagc gtgaggtgct ggtcaccgcc ggagcgaccg agggcatcgc
    10621 ggccgccatg ctgggcctgc tcgagcccgg cgatgaggtg ctgctgctcg agccctacta
    10681 cgactcgtac gcggccacca tcgcgctggc cggtgccgtg cgacgtccgg ttcggctcga
    10741 agccccggac ttcgcactgc cgcgagagcg cctcgccgcc gccgtcgggc cgcgcacccg
    10801 gctgctgttg ctgaactcgc cgcacaatcc gaccggcatg gtgctcagtg ccgacgacct
    10861 ggcattcgtg gcctcgttgg cggtgcggca cgacctgatc gtggtcaccg acgaggtgta
    10921 tgaacacctc atcttcgacg gcgtctcgca cgtgccgctg tgcaccctgc cgggaatgtt
    10981 cgatcgcacc ctcacgatca gctccgcggg caaggcgttc tcggtgaccg ggtggaagat
    11041 cggctgggtg agcggcccgg cggagctggt ggccgcggtg cgggccgcca agcagtttct
    11101 tacctacgtc aacggggcac cctttcagcc ggcggtggcg gaggcgctcg gactggacga
    11161 tgccttcttc accacccagc ggcgggcgct gcaagccaag cgtgatcggt tgacggacgg
    11221 cctgcgcacc gccggcttcg ccgtgacccg cccgtcgggc acctacttcg tcgtcgcgga
    11281 gggccgtcct ctcggtttcg acgacggcta cgcgctgtgc cgcgaactgc ccgagctgtg
    11341 cggggtggtc gcggtgcccc tgtcggtgtt ctacgacgac gcggacggag tcggcagcct
    11401 ggtgaggttc gcgttctgca agcgcgacga ggtgctggac gaagcggtca cgcggctggc
    11461 gcggctcgcc ggctgacccg ggtcccgttg cggcgcctca ccggctacag cctgcatcga
    11521 ccggggggcc acaccgatcc cggatccagg tcgaagcgcc agccggctcg gagcggctcc
    11581 cctccgcccg cgtgccaggt cgagcgccac cggttcggag cgcacccatc catgcgccct
    11641 ttggtcggtc actctcagca gaggcccggt gtgctctcgg gtgggccgga tttctgcccc
    11701 gtgcggcacg agttggtgag ctggcttcgg tgggtcgtgc tggagcgggt gggttttgct
    11761 accgcagtcg gtgtcaaaac ccacccgcaa agaagaacct gatcaatcct gggccgagct
    11821 tgtcgcgacc gtccatgcgg ggcttccagt tatcgacacg ctcaaccaac gactcgtgaa
    11881 cgagcgctct gcacccgggc ccacctatgc cgaccgactg cgtgaccccg tccggtcgcc
    11941 cgctgcgagg cccgcgccgg ccaggcgtcc gatcgtccgc gcgcgccggc gtgctgcccg
    12001 accgtcgtca gccgctcggg acgcctgcca gcggatccgt gctcaggcgg ctctggccga
    12061 ggtgtcgatc aggccgagcc gttcgaaggc gaccaccagg ccgtcccgct cgggcggggg
    12121 cacgatcagg tcggcaccgg cgagcaactc ggggacgccg ccctcgatcg ccactccggt
    12181 accggcgtag gccaccatct cgagatcgtt gtggctgtcg ccgatggcga tgatgtcgtc
    12241 gcggtcgatg ccgaagtgct gctcgaccac cgcgatgccc accgacttgt tgacgtcgct
    12301 cagaaagaac tcgccgccgc gctcacccag cgacccgatc gaactcggca ccacagccac
    12361 ctcgggcgcg agttgggcac cgatccagct ggtcggaacg gcgcaatcga agcaggtgat
    12421 cttagcgaac gattcggacg ccgggtcgtc cgagacccgt agcgggtcaa gaatgccgtc
    12481 cgccgcttcg ccgaagctcg gcccgtgctc ggcgccggtc agcagggcga ccaaccgctg
    12541 cttcatgccc ggaccgacgt acgcggcgtc cggtccctcg agaatgtaga ccgcctcgtt
    12601 ggcgtccagc agcgtgatgg tgcgcgccgc caactccgac gagaagcgcc ggtcggcgag
    12661 cacctcgccc tcgacctcga cgtagccgcc cgcggccgcg accaggccgt cgaagacctc
    12721 gacatcctcg ggggtgacca gtgccttcgg acgcccggtg cacagcagca cccgatggcc
    12781 gttgcgctgc gcctcacgca ctgcccggac gtgcgccggt ggcaccaccc cgttgtgtgc
    12841 ataggttccg tcgaaatcga gaaatacggt tttgcccacg agggggagca ctcctttggc
    12901 ggtgaagccc gctggtatcg gcgaagcagt agctgttgaa gcttgtacta cttcattcta
    12961 gccgatccga cgaaaatacg tggacgcccc ggccggcggc cgtgctcgga cgccggccgg
    13021 gtgcggaatc acgccgagta gtccgcggcc agcgaggcca acaaccgcgc gccgtagccg
    13081 gtggctccgg atgagcgcca cgagtcgtcg gcatcggtcg acatggtgcc ggcgatgtcg
    13141 atgtgcgccc agggcgtgcc gtccacgaac tcctccagga acaacgccgc cgtggtgcag
    13201 ccggcgaagc gtccgcccag attggcgatg tcggcgatct cggagtcgag ttgcttgcgg
    13261 tacttgcgct cgagcggcag ttgccacacc ggttcgtccg tggcggcggc cgcggcgctc
    13321 acccgatcga ccatcgcctg gtcgttgccg agaatggcgg ctcgcgactg gcccagcgcc
    13381 atcagggccg cgccggtcag ggtggcgatg tcgacgatcg cgtcgacctt ggcctcgacg
    13441 gccagggcga gaccgtccat cattgccagc cggccttcgg cgtcggtgtt cttcacctcg
    13501 acggtggtgc cgttgcgaca ggtcagcacg tcgcccagct tgtacgaact gcccgagggc
    13561 atgttgtcgg tgcagcacaa ccaccccgtg acggccgccg tggcgccgag atcgcgcaag
    13621 gcggtgaacg cgccgagcac ggccgccgcg ccgcccatgt cgatcttcat cagcaggtgc
    13681 atggggtcgg acggtttgag gctgatgcgc ccgagtcgta catgatgccc ttgccgacca
    13741 ggcccaggtg ccccgacggc tcaccctgcg ggctgtagct gagcacgatc aggcggggct
    13801 cttcggcgct gcccgcgttg accccgagaa tg
//
LOCUS       DAOSUG010000011        13586 bp    DNA     linear   ENV 10-JUN-2024
DEFINITION  MAG TPA_asm: Micropruina sp. isolate AMR_MDS_2195
            NODE_2714_length_13586_cov_5.587909, whole genome shotgun sequence.
ACCESSION   DAOSUG010000011 DAOSUG010000000
VERSION     DAOSUG010000011.1
DBLINK      BioProject: PRJNA925477
            BioSample: SAMN32864262
            Sequence Read Archive: SRR4236673
KEYWORDS    WGS; ENV; Metagenome Assembled Genome; MAG; Third Party Data; TPA;
            TPA:assembly.
SOURCE      Micropruina sp. (wastewater metagenome)
  ORGANISM  Micropruina sp.
            Bacteria; Bacillati; Actinomycetota; Actinomycetes;
            Propionibacteriales; Nocardioidaceae; Micropruina.
REFERENCE   1  (bases 1 to 13586)
  AUTHORS   Abdulkadir,N., Saraiva,J.P., Zhang,J., Stolte,S., Gillor,O.,
            Harms,H. and Rocha,U.
  TITLE     Genome-centric analyses of 165 metagenomes show that mobile genetic
            elements are crucial for the transmission of antimicrobial
            resistance genes to pathogens in activated sludge and wastewater
  JOURNAL   Microbiol Spectr 12 (3), e0291823 (2024)
   PUBMED   38289113
REFERENCE   2  (bases 1 to 13586)
  AUTHORS   Abdulkadir,N.
  TITLE     Direct Submission
  JOURNAL   Submitted (24-MAR-2023) Environmental Microbiology, Helmholtz
            Center for Environmental Research -UFZ, Permoserstr 15, Leipzig
            04318, Germany
COMMENT     The annotation was added by the NCBI Prokaryotic Genome Annotation
            Pipeline (PGAP). Information about PGAP can be found here:
            https://www.ncbi.nlm.nih.gov/genome/annotation_prok/
            
            ##Genome-Assembly-Data-START##
            Assembly Method        :: SPAdes v. 3.11.1
            Genome Representation  :: Full
            Expected Final Version :: Yes
            Genome Coverage        :: Not Applicable
            Sequencing Technology  :: Illumina MiSeq
            ##Genome-Assembly-Data-END##
            
            ##Genome-Annotation-Data-START##
            Annotation Provider               :: NCBI
            Annotation Date                   :: 05/04/2023 04:57:10
            Annotation Pipeline               :: NCBI Prokaryotic Genome
                                                 Annotation Pipeline (PGAP)
            Annotation Method                 :: Best-placed reference protein
                                                 set; GeneMarkS-2+
            Annotation Software revision      :: 6.5
            Features Annotated                :: Gene; CDS; rRNA; tRNA; ncRNA
            Genes (total)                     :: 2,776
            CDSs (total)                      :: 2,750
            Genes (coding)                    :: 2,714
            CDSs (with protein)               :: 2,714
            Genes (RNA)                       :: 26
            tRNAs                             :: 24
            ncRNAs                            :: 2
            Pseudo Genes (total)              :: 36
            CDSs (without protein)            :: 36
            Pseudo Genes (ambiguous residues) :: 0 of 36
            Pseudo Genes (frameshifted)       :: 2 of 36
            Pseudo Genes (incomplete)         :: 32 of 36
            Pseudo Genes (internal stop)      :: 2 of 36
            ##Genome-Annotation-Data-END##
FEATURES             Location/Qualifiers
     source          1..13586
                     /organism="Micropruina sp."
                     /mol_type="genomic DNA"
                     /submitter_seqid="NODE_2714_length_13586_cov_5.587909"
                     /isolate="AMR_MDS_2195"
                     /isolation_source="Activated sludge of municipal
                     wastewater treatment plants"
                     /db_xref="taxon:2737536"
                     /environmental_sample
                     /geo_loc_name="Canada"
                     /lat_lon="49.25 N 123.1 W"
                     /collection_date="2015-06-16"
                     /metagenome_source="wastewater metagenome"
                     /note="metagenomic"
     gene            complement(<1..282)
                     /locus_tag="PLF56_00745"
     CDS             complement(<1..282)
                     /locus_tag="PLF56_00745"
                     /inference="COORDINATES: ab initio
                     prediction:GeneMarkS-2+"
                     /note="Derived by automated computational analysis using
                     gene prediction method: GeneMarkS-2+."
                     /codon_start=1
                     /transl_table=11
                     /product="hypothetical protein"
                     /protein_id="HOQ52119.1"
                     /translation="MKKIATLVASGALVAASLIATAPQASAACLGLEKWTATAPGSSA
                     VWDLDGASACQDLNAAYTYSHNDKVKGWYKQSGTWREGTRGFVAVTTSDS"
     gene            821..1906
                     /locus_tag="PLF56_00750"
     CDS             821..1906
                     /locus_tag="PLF56_00750"
                     /inference="COORDINATES: protein motif:HMM:NF019350.2"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_component: GO:0016020 - membrane [Evidence IEA];
                     GO_function: GO:0000155 - phosphorelay sensor kinase
                     activity [Evidence IEA];
                     GO_function: GO:0046983 - protein dimerization activity
                     [Evidence IEA];
                     GO_process: GO:0000160 - phosphorelay signal transduction
                     system [Evidence IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="histidine kinase"
                     /protein_id="HOQ52120.1"
                     /translation="MLNRQPALGRFLNGGFDVLLAAALAVLASVAHATRGEMTPAIID
                     LAACLSAALTTKWPRVAGVALGAVLLTLLFTPPQWSSLGEYAALIPILGTGIRGQRRT
                     RAWMTVYYGAVLAALTYEDVSGGPVVVFGILAWAALIAVLWGIGNLFTAYRDAQAKAH
                     AAALQGQRLSVARDLHDTVARELTRASLHAQAAFDAQPTPALESVVTGIQRASAELRW
                     MLALLRDPYPALPTDTLNGTAPEALVEATAALRARGFTITTTADGNLDMIPAALLPTV
                     RAVIGEACANIERHADPEQPCAIIVSVSGNTLDALFLNGLGAGEDAPKSGGMGLVGLR
                     ERLALVGGELGTEQEGSQWIARATIPL"
     gene            1876..>2416
                     /locus_tag="PLF56_00755"
     CDS             1876..>2416
                     /locus_tag="PLF56_00755"
                     /inference="COORDINATES: protein motif:HMM:NF012301.2"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_function: GO:0003677 - DNA binding [Evidence IEA];
                     GO_process: GO:0000160 - phosphorelay signal transduction
                     system [Evidence IEA];
                     GO_process: GO:0006355 - regulation of DNA-templated
                     transcription [Evidence IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="response regulator transcription factor"
                     /protein_id="HOQ52121.1"
                     /translation="MDRPRHHPTLNPRITVMLVDDDLLVRTTASDLLGRHRDLAVIGV
                     FADGHDALLAAATSPPDVMVVDISMPAMSGSELTRRVREQHPATHVLAYTSLADEQSL
                     SEMLNAGAAGVVYKEASVGSVADAIRATHVGLSVLSPRFSSRLARPELEEPLTDTETD
                     ILRLVSRGMTNEQIGDHINL"
     assembly_gap    2417..2426
                     /estimated_length=10
                     /gap_type="within scaffold"
                     /linkage_evidence="paired-ends"
     gene            complement(2626..3900)
                     /locus_tag="PLF56_00760"
     CDS             complement(2626..3900)
                     /locus_tag="PLF56_00760"
                     /inference="COORDINATES: protein motif:HMM:NF012798.2"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_function: GO:0003677 - DNA binding [Evidence IEA];
                     GO_process: GO:0006310 - DNA recombination [Evidence IEA];
                     GO_process: GO:0015074 - DNA integration [Evidence IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="tyrosine-type recombinase/integrase"
                     /protein_id="HOQ52122.1"
                     /translation="MGQGRLEVVAVTQRRRGFGRVRRERSGRYSAAYVGPDLHLHRAP
                     ETFHAKVDAEGWLATERRLIDLDQWSSPQSRRPTYVDPEPPPGGVTVGVYATAWLEDR
                     CERDPHDPRALRPSSVKDYRLLLSNHIVPGLGGIPLAELNAGQVEAWYRKMGALRIPR
                     ARAKAYSLLRTILNAAKNDPDVPLSENPAHIKGAGRVPRSTQIHPATLEELAAITTAM
                     PAELRLAVQLGAWCALRYGEVFELRRKDIDPVQGVVHIRRAVVWTKGQVTNDRPKTSA
                     GVRDVTIPPHVLPMVTDHLKRYVAKRPDALLFPDSEGRNLRPSGFQAAWHAARAAAGR
                     DDLKFHHLRHTGAVLAAQSGATLADLMGRLGHTTPAMAMIYQHTAADRDRLIAERLSQ
                     VALAIPGKQTTAARRTAQERPSGSGQPSLLDA"
     gene            complement(4365..4883)
                     /locus_tag="PLF56_00765"
     CDS             complement(4365..4883)
                     /locus_tag="PLF56_00765"
                     /inference="COORDINATES: protein motif:HMM:NF022321.2"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="DUF2703 domain-containing protein"
                     /protein_id="HOQ52123.1"
                     /translation="MTDLTIAWQRLVDAGQTCPRCGDTGSEVRTAAATLTAALAPLGI
                     TVRLAEATINLAQFEQAPPESNRILIAGRPLEDWLGGATSQSPCCDVCGPNDCRTITV
                     DDTTYETVPAGLITRAGLLATAELLAQPSASHDDPTQETTGTTETCCGPTAEPTDLPN
                     RSLPLATKAGCC"
     gene            complement(4880..6649)
                     /gene="arsA"
                     /locus_tag="PLF56_00770"
     CDS             complement(4880..6649)
                     /gene="arsA"
                     /locus_tag="PLF56_00770"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_008598606.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_function: GO:0005524 - ATP binding [Evidence IEA];
                     GO_function: GO:0015446 - ATPase-coupled arsenite
                     transmembrane transporter activity [Evidence IEA];
                     GO_function: GO:0016887 - ATP hydrolysis activity
                     [Evidence IEA];
                     GO_process: GO:0071722 - detoxification of
                     arsenic-containing substance [Evidence IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="arsenical pump-driving ATPase"
                     /protein_id="HOQ52124.1"
                     /translation="MKFLEDMPRFVFFTGKGGVGKTSLACATAVHLAGAGRRVLLVST
                     DPASNVGQVFDQPIGNRVTPVVGVEGLDALEIDPESAAEQYRERILGPVRAALPASEI
                     ASLTEQLSGSCTTEIASFNEFTGFLAEEDSHYDHVVFDTAPTGHTIRLLQLPGDWTGF
                     IDSGGDASCLGPMSGLDKHRDQYARALAALTDPTTTRLVLVARAQATSLAEAERTASE
                     LAGIGIHATHLVVNAVLPADGHPDPLAAATRARETAALAAVAGPLADLPQDRIPLKTV
                     NMVGVLALRALLSPEDTAEAPTDAAPLPAGGLPGLDALVDELAAIGHGLVMCVGKGGV
                     GKTTIAAALAVGLAARGHRVHLTTTDPAAHLDATVTETPDLPSLTLSRVDPAQAIADY
                     RAHVMATQGQGLDADALAALAEDLQSPCHQEIAVFQQFSSLVGQARRQFVVMDTAPTG
                     HTLLLMDTTGAYHRDTVKTMAAGVRFTTPLMRLQDPDYTRLVMVTLPEPTPVLEAMAF
                     RDDLARAGITPWAWVINQSLTAARPTAPLLLRRARLETDPIDTVTKTAARVAVVPVLL
                     DEPVGAARLLALTDTHTMTGSTP"
     gene            complement(6686..6922)
                     /locus_tag="PLF56_00775"
     CDS             complement(6686..6922)
                     /locus_tag="PLF56_00775"
                     /inference="COORDINATES: protein motif:HMM:NF024590.2"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_function: GO:0015036 - disulfide oxidoreductase
                     activity [Evidence IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="thioredoxin family protein"
                     /protein_id="HOQ52125.1"
                     /translation="MHIKVLGPGCANCVNLEKNTRAALASLGMEASVEKVTDYADIAG
                     YGIMRTPGLVVDEQVVLSGRVAKPAEIAQLLAGR"
     gene            complement(6968..8014)
                     /locus_tag="PLF56_00780"
     CDS             complement(6968..8014)
                     /locus_tag="PLF56_00780"
                     /inference="COORDINATES: protein motif:HMM:NF015715.2"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_component: GO:0005886 - plasma membrane [Evidence IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="permease"
                     /protein_id="HOQ52126.1"
                     /translation="MTTRLGRPGLRLSLGVAGASVAWVALYWVNEPFWTWLIGEAAGL
                     DLESRLGSGVWFFFYDTIKIALLLTGIIFVVTVLRSFMSVERTRALLGGKREGVGNVL
                     AALLGIVTPFCSCSAVPAFIGFVAAGVPLGVTMSFLIASPLINEVAIALLLGLFGPGP
                     TLLYVAAGVSIAIVAGWVIGRLRLERWVEPFVFETRLRGQVIDSTAHLTWPDRVQLGI
                     DEVGTILRKIWPYLLVGIGLGAVIHGWAPENFFATYAGRDNPFAVLIAVVVGVPLYSN
                     AAGVLPLVDALHDKGLPMGTLLAFMMATVALSLPEMILLRRVLKPKLIATFVAVVAAG
                     IIAVGYLFNAVLPV"
     gene            complement(8011..8331)
                     /locus_tag="PLF56_00785"
     CDS             complement(8011..8331)
                     /locus_tag="PLF56_00785"
                     /inference="COORDINATES: protein motif:HMM:NF033788.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_function: GO:0003677 - DNA binding [Evidence IEA];
                     GO_function: GO:0003700 - DNA-binding transcription factor
                     activity [Evidence IEA];
                     GO_process: GO:0006355 - regulation of DNA-templated
                     transcription [Evidence IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="metalloregulator ArsR/SmtB family transcription
                     factor"
                     /protein_id="HOQ52127.1"
                     /translation="MVESIEAELTRDAATLLAVAADPVRLTLLNRLAASGTRCVCDLQ
                     LDPPIPANLLSYHLRMLREAGLVTTARRGRWVDYTLAEDALERLHAALPVAAATRAEC
                     GSCR"
     gene            complement(8396..9406)
                     /locus_tag="PLF56_00790"
     CDS             complement(8396..9406)
                     /locus_tag="PLF56_00790"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_020391168.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="permease"
                     /protein_id="HOQ52128.1"
                     /translation="MLDHLTALVAGVATDVATALAHNWPFLLASVVVASVVQVYVKPD
                     RLGRWLRRRTWVAILGAVALGALTPFCSCGTTAVVLGAMASSVPWAPVVAFMVSSPLT
                     SPQEYVWSVGLFGPAFATTFFGAAIAVGLIGGGAAWWLERHGLLAGQARLATPTPDGG
                     CDTGCQTDAVAGAPGTTRVGGTRQLAVIDARPATVVDAKPATWRRFGVALATNGRRLA
                     VYFLGFAALGYLVIRILPTGLLTTWLGAGNPVWSVPLAALLGVPVYLNTDASLPLVAT
                     LLDGGMAPGAGIAFLITGAGTSVGAISGMLVIARWRIVALVIGTLLVTATAAGWLAGL
                     WL"
     gene            9473..9850
                     /locus_tag="PLF56_00795"
     CDS             9473..9850
                     /locus_tag="PLF56_00795"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_020575167.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_function: GO:0003677 - DNA binding [Evidence IEA];
                     GO_function: GO:0003700 - DNA-binding transcription factor
                     activity [Evidence IEA];
                     GO_process: GO:0006355 - regulation of DNA-templated
                     transcription [Evidence IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="metalloregulator ArsR/SmtB family transcription
                     factor"
                     /protein_id="HOQ52129.1"
                     /translation="MSLQEAAPRPTSVGDLYCSPILQAPLQPDDALALAGALKVLADP
                     ARLRLLSLIRAADGGRATTRVLAGRLGLTQPTVTHHLGALFDAGFLDRERVGRQTWYS
                     VNTDGLRAIQQLLAPTPVGTVES"
     gene            complement(9951..12083)
                     /locus_tag="PLF56_00800"
     CDS             complement(9951..12083)
                     /locus_tag="PLF56_00800"
                     /inference="COORDINATES: protein motif:HMM:NF019604.2"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_function: GO:0016491 - oxidoreductase activity
                     [Evidence IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="FAD-dependent oxidoreductase"
                     /protein_id="HOQ52130.1"
                     /translation="MPMPASPRAQRLVIVGAVAAGTSVGAKARRNDEEMEIVIYERDR
                     DISYSGCGLPYFIGGTVPDADDLHPRDAAWFAKRYNIDIRTGHEVISADPAGRTLQVR
                     DLSSGEVTADRYDSLVLATGATSIIPPVPGVDLPGVFALRSVRDAEAIKGWLDDASPE
                     QVVVVGSGFIGLEVAEQLVASGIGVTLVERLPQVMPALDADMAFRVQEELERHGVTVR
                     LNTSMTGLEGDTRVCGVRLDTGELIAAQLVILAVGVRPNTDLARDLGVTIGETGAIAV
                     DPSMRTNVEGVFAVGDCAESYSVITGRPLWRPLGSTANKMGRIAGDQITGGTTLEHRG
                     ILGTGIVRVFELAVGHTGLTETQARAEGFDPVVLHNTKPAHATYLGGKELTIKALADR
                     ASGRLLGAQAVGPQGVDKRIDVLATAITFGATVADLFHLDLAYSPPFATTKDPVHYTG
                     MALETAVRGRAPLISPTDLDERLARGERIQIVDVRAAKDYAKSHLPGAVNIPLADLRR
                     RVGELDPQVPTVTYCNKGVTGNAAQNVLLALGLAEVMNLSGGNSTHQTHTRQLQRATS
                     LPSTIKPSHLPHVLFLCVHNAGKSQMAGALMRHLYGDRIVVTTAGTGPDDAVDDASAR
                     IVAELGASTAGENPKAVTAAMLDAADRIILIGPDVQLNPPEPLADRVERWPIHDPAED
                     GIEGDERTRNIRDQIANRVHALATELTS"
     gene            12258..12926
                     /locus_tag="PLF56_00805"
     CDS             12258..12926
                     /locus_tag="PLF56_00805"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_008363849.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="helix-turn-helix domain-containing protein"
                     /protein_id="HOQ52131.1"
                     /translation="MVLGDVNARARAYAALGDPSRLAIIDLLAHADLASGELAAQLEL
                     PTNLVAHHLGVLEQAGLVTRHRSEGDGRRSYVQRTPACDRLLGATPLPRPARVAFVCS
                     QNSARSQLAESYWRSISDIPALSAGTRPARRVHPGAVSVARRHGLDLTHATPKLARDV
                     LTGDELLIAVCDRAYEDVDGPLLHWSIPDPAVKEDPHAFDAAYDDITQRIDRLASSLE
                     GEPR"
     gene            12923..13579
                     /locus_tag="PLF56_00810"
     CDS             12923..13579
                     /locus_tag="PLF56_00810"
                     /EC_number="1.20.4.4"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_019044557.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_function: GO:0030612 - arsenate reductase (thioredoxin)
                     activity [Evidence IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="arsenate reductase ArsC"
                     /protein_id="HOQ52132.1"
                     /translation="MTVHPAQERSWEGLHLDDRLALKQAAAHLAQEFSGLYGTQTIER
                     FMDSSFDQFAGRATVTKFLPLLAERFARQRLHALARVEGLHDDGKPVVLFLCTHNAGR
                     SQMALGFFQALAGDNAVAWSGGSEPGLEVNPVAVQAMAERGIDIAHEFPKPWTDETVR
                     AADVVITMGCGDACPIFPGKRYESWELDDPHGQDLPAVRPIRDDVERRVRSLLASLNL
                     "
BASE COUNT         2089 a         4577 c         4772 g         2138 t
ORIGIN      
        1 cgagtcagac gtcgtcaccg ccacgaagcc acgggtgcct tccctccaag tccccgactg
       61 cttgtaccac cccttgacct tgtcgttgtg ggagtacgtg taggcggcgt tcaggtcctg
      121 gcacgcactg gctccgtcaa gatcccacac agccgaactg ccaggagcgg tggcggtcca
      181 tttctccagg cccaggcaag cggcgctggc ctggggtgcg gttgcgatca ggcttgcagc
      241 caccaacgcg cctgaggcca ccaacgttgc gatcttcttc atgacgagtt cccttcctct
      301 agctgatgcg aaaacggacg cggtgagagc cagctggcgt caccggggag gtgtccgtcc
      361 gacttcgggg cgaatcaacc tcgcgatgtg agtctgatgc gaaatccccc tgccgatccg
      421 gccgatcgtc ccgaacctga ccgatcaggc aggatcgcgg gcaacaactg gtcgaacgga
      481 tacatcccgg ggcctcagct cagtgaaggc agccgggcca cctggggatg atgtcgcaga
      541 tgcccgcggg aacccggggc gtcgtagtga tggtccgggg cgacaacccc ggtagcgcga
      601 tgatcgcagt ggcgaccatg caggcgaagg ccgtggcccc ggaaaggcgt gtgtgcatga
      661 tccgatcgtg gcggctgcgc gaccgcgaca gatccgatac cgccagacct gctgaccgat
      721 cgggctactt ggttgtccag aactgtccgg tcgggcagtc cactgttcag ggcggccgcg
      781 cgcgcgacag ttagagtgat catggataga gggagactga atgctgaatc gacagcccgc
      841 actcggccga ttcctcaacg gcggatttga cgtgttgctc gctgccgcgc tagccgtcct
      901 tgcctcggtg gcgcatgcga cgcgaggcga gatgaccccg gcgatcattg atctggccgc
      961 ctgtctctcg gccgccctca cgacgaagtg gccccgtgtt gctggcgttg cccttggggc
     1021 ggtgctgctg accctgctgt tcacccctcc tcagtggtcc tcgctgggcg agtacgccgc
     1081 gctgattccg atcctcggca caggcatacg cggccagcga cggacacgtg catggatgac
     1141 ggtctactac ggggcggttc ttgctgcgtt gacttacgag gacgtctcgg gtggcccagt
     1201 ggtggtgttc gggatcctcg cttgggccgc gttgattgcg gtgctgtggg gaatcggcaa
     1261 cctcttcacg gcctaccgtg atgcacaggc caaagctcat gcagccgcgc tgcaaggaca
     1321 gcggctcagc gtggcacgcg accttcacga cacggtcgcc cgagagttga cccgagcgtc
     1381 tctccacgcc caggcggcat tcgatgcaca gccaacgccg gctctggaga gcgtagtgac
     1441 cggcatccag cgtgcctccg cggaactccg ctggatgctc gcactcctgc gcgacccgta
     1501 tccagcactg cccaccgaca ctctcaatgg cacagctccc gaggctcttg tcgaagccac
     1561 cgccgccctc cgcgctcgcg gcttcaccat caccacgaca gcggacggga atctcgacat
     1621 gatcccggcc gcgctgctcc ccaccgtccg cgcggtcatc ggtgaggcct gcgcgaacat
     1681 tgaacgtcac gccgacccgg aacaaccgtg cgccatcatc gtcagcgtga gcgggaatac
     1741 tctggacgct ctctttctca acgggctcgg cgcgggggag gacgcaccta agtcgggcgg
     1801 gatgggactc gtgggcctcc gagaacgcct cgcgctcgtc ggcggagagc tcggaaccga
     1861 acaggagggc tcccaatgga tcgcccgcgc caccatccca ctctgaaccc gcgcatcacc
     1921 gtgatgctcg tcgacgacga tctcttggtt cgcaccaccg cgtccgacct gcttgggcgc
     1981 catcgcgacc tagctgtcat cggcgtcttc gccgacgggc acgacgcact gctcgccgcg
     2041 gccaccagcc cgccggatgt catggttgtg gacatctcta tgccagcgat gagcggctct
     2101 gaactcaccc gccgcgttcg cgaacaacac ccggcgacgc acgtcttggc ctacacgtca
     2161 ctcgcagacg aacagtcgct ctcggagatg ctcaacgccg gagctgccgg cgtggtctac
     2221 aaggaagcct cagtcggttc ggttgcagac gcgatccggg ccacccacgt cgggctgtcc
     2281 gtactttcgc cccgcttcag cagccgactt gcccgacccg aactcgagga gccgctcact
     2341 gataccgaaa ccgacattct tcgcctcgtc agccgcggca tgaccaacga gcagatcggc
     2401 gaccacatca atctgannnn nnnnnnaagg ctcggcgcca ataaccgtgt caccctcgcc
     2461 gtagcggcag tgcatctggg acttgtcggg cggtcacaag cagaacggcg accgaggcca
     2521 ggtccgtcgc cgactactcg acctgactga agtcctgccg aggcacaggc gcgaagccag
     2581 gcggctcgcg caccttctgg cgctcccccg cgttgtgcct gcccatcatg cgtccagcag
     2641 gctgggctgt ccggagccgg agggtcgctc ttgcgccgtc cgccgtgccg ctgtggtctg
     2701 cttgccgggg atggccagcg cgacctggga gaggcgctcg gcgatgaggc ggtctcgatc
     2761 ggcagcggtg tgttggtaga tcatcgccat cgccggggtg gtgtggccaa ggcgtcccat
     2821 caggtcggcc agggttgcgc ccgactgcgc ggccagcacc gccccggtgt ggcgcaggtg
     2881 gtggaacttc agatcatcgc gtccagcggc ggctcgggcg gcgtgccagg cggcctggaa
     2941 gcccgaagga cgcaggttgc ggccttcgga gtccgggaac aacagggcgt ccggacgctt
     3001 ggcgacgtac cgcttgaggt ggtcggtgac catcggcagc acgtggggag ggatggtgac
     3061 gtcgcggacg ccggcgctgg tcttgggtcg gtcgttggtg acctggccct tggtccacac
     3121 gacggcccgg cgtatgtgca cgacgccctg caccgggtcg atgtccttgc ggcgcagttc
     3181 gaacacctcg ccgtaccgca gggcacacca agcgccgagc tggacggcca gccgcagctc
     3241 cgcgggcatc gcggtcgtga tcgccgccaa ctcctccaaa gtggcggggt ggatctgggt
     3301 cgagcgtggg acgcgtccgg cgcccttgat gtgggccgga ttctccgaga gtggcacatc
     3361 gggatcgttc ttggccgcgt tcaggatcgt ccgcagcagg ctgtacgcct tcgccctagc
     3421 ccgcggaatc cgaagggcgc ccatcttccg gtaccaggcc tccacctggc cggcgttcaa
     3481 ctcggccaac gggatgccgc ccagaccggg cacgatgtga ttggacagca gcagccggta
     3541 gtccttcacc gaggacggac gcagtgcccg cgggtcgtga gggtcacgct cgcagcggtc
     3601 ctccagccac gcggtcgcgt acacacctac cgtgacgccg cccggcggcg gttcggggtc
     3661 gacgtaggtc ggccgacggg actgcggcga cgaccactgg tcgaggtcga tcaagcggcg
     3721 ctcggtcgcc agccagccct ccgcgtcgac cttggcgtgg aaggtctcag gcgcccgatg
     3781 gagatggagg tccgggccga cgtaggccgc gctgtaccgg ccagaacgct cccgccgaac
     3841 ccgcccgaaa ccccttcttc gctgtgtcac cgccaccacc tccagccgtc cctggcccac
     3901 gagcagaccg tgagctgcgg gatcttgcac aacatatgca caacatagga gcccacgtga
     3961 gcccgttctt gcccacttct gcgcacctat ttcgaccccg tttcgggacc atcataccta
     4021 gtcagaagcg tgttcactag gcccgatagg caatcctcgc aattcgcata atagaggttc
     4081 aactcctctc tcgcgcacaa aaagtgcctc tgacaagggc aaataggacg aagaggcggg
     4141 aggcgaaaac ctctcgcctt tcctatgtcc ggggcgtgtg gcgtgctgct ggcaagggcg
     4201 aatgcttcgc tgaggtcgcc ggcgcttcct ctggctggcc gacaagttcc ccacgcgtgg
     4261 gggtgttccc cacgcattcc ccacgagttc cccacgccgt gagggatggc cttgcgacgc
     4321 gtccagggac gccttcccgg accggtctcg ctgggttagg cctctcagca gcagcctgcc
     4381 ttggttgcca gcggcagtga acggttcggg aggtcggtgg gttcggcggt gggtccgcag
     4441 caggtctcag ttgtgccggt ggtttcctgg gtggggtcgt cgtgtgacgc gctgggctgg
     4501 gcgagcagtt cggccgtggc gaggaggccg gcgcgggtga tgaggccggc gggcacggtt
     4561 tcgtaggtgg tgtcgtcgac ggtgatggtg cggcagtcgt tggggccgca gacgtcgcag
     4621 caggggctct ggctggtcgc gccgccgagc cagtcctcga ggggtcggcc cgcgatcagg
     4681 atgcggttgg actccggtgg cgcttgctcg aactgggcca ggttgatggt ggcctcggcc
     4741 aggcgcacgg tgatgcccag gggggcgagg gccgcggtga gggtcgcggc tgccgtgcgt
     4801 acctcgctgc cggtgtcgcc gcagcggggg caggtttggc cggcgtccac gaggcgttgc
     4861 caggcgatgg tgaggtcggt catggggtac tcccggtcat ggtgtgggtg tcggtgaggg
     4921 cgaggagtcg ggcggcgccc acgggttcgt cgagcaggac gggcacgacg gcgacccggg
     4981 cggccgtctt cgtgacggtg tcgatggggt cggtctccag gcgggcgcgg cgaagcagca
     5041 gtggggcggt cggcctggcg gcggtgaggg attggttgat gacccacgcc cagggtgtga
     5101 tgccggcgcg ggcgaggtcg tcgcggaacg ccatcgcctc cagcaccggg gtcggttcgg
     5161 gcagggtcac catgaccagg cgggtgtagt cggggtcctg caggcgcatc agcggagtgg
     5221 tgaaccggac gccggccgcc atggtcttga cggtgtcacg gtggtaggcg ccggtggtgt
     5281 ccatcagtag cagggtgtgc ccggtgggtg cggtgtccat gaccacgaac tggcgacggg
     5341 cctgcccgac cagggaggag aactgctgga agacggcgat ctcctggtgg catgggctct
     5401 gcaggtcctc ggcgagggcg gccagcgcgt cggcgtccag gccctggccc tgggtggcca
     5461 tcacgtgggc ccggtagtcg gcgatcgcct gggcggggtc gacccgcgac agcgtcaacg
     5521 acggcaggtc gggggtctcg gtgacggtgg cgtccaggtg cgccgcgggg tcggtggtgg
     5581 tcaagtggac ccggtgaccg cgtgcggcca gcccgaccgc gagcgccgcc gcgatggtgg
     5641 tcttgccgac cccaccttta cccacacaca tcaccagccc gtgaccgatg gcggcgagtt
     5701 cgtcgacgag ggcgtccagg cccggcagtc ctccggcggg cagtggggcg gcgtccgtcg
     5761 gagcctcggc ggtgtcttcg ggtgacagca gtgcgcgcag ggcgagcacg ccgaccatgt
     5821 tcacggtctt cagcgggatc cggtcctgtg gcaggtcggc gagcgggccc gcgacggcgg
     5881 cgagcgcggc cgtctcgcgg gcgcgggtcg cggcggcgag agggtcgggg tggccgtcgg
     5941 cgggcaggac ggcgttcacc acgaggtggg tggcgtggat cccgatcccg gccagttcgg
     6001 acgccgtgcg ctcggcctcg gcgagcgagg tggcctgcgc ccgggccacc agcaccagcc
     6061 gggtggtggt ggggtcggtg agggccgcca gcgcgcgggc gtactggtcg cggtgtttgt
     6121 ccagccccga catcggcccc aggcaggacg cgtcgccgcc cgaatcgatg aagccggtcc
     6181 agtcgccggg cagctgaagc aaccggatgg tgtgtccggt cggcgcggtg tcgaacacca
     6241 catggtcgta gtgggagtcc tcctcggcca ggaagccggt gaactcgttg aacgatgcga
     6301 tctcggtggt gcacgacccc gacaactgtt cggtgagcga cgcgatctcg ctggccggca
     6361 gcgcggcccg gacggggccg aggatccgtt cccggtactg ctcggcggcc gattcggggt
     6421 cgatctccag cgcgtccagg ccctcgacgc cgaccaccgg ggtgacccgg ttcccgatcg
     6481 gctggtcgaa cacctggccc acgttggagg cggggtcggt cgagaccagc agcacccggc
     6541 gccccgcccc ggccaggtgg accgccgtcg cgcacgccag cgacgtcttg cccaccccgc
     6601 ccttgccggt gaagaacacg aaccgtggca tgtcctccag gaacttcatg gcaaactccc
     6661 tccagccgat cgcggggtca gccggtcagc ggccggccag cagttgggcg atctcggcgg
     6721 gcttggcgac ccgacctgac agcactacct gctcgtcgac caccaggccg ggggtgcgca
     6781 tgatgccgta gccggcgatg tcggcgtagt cggtgacctt ctcgacgctg gcctccattc
     6841 ccagagacgc caaggctgcg cgggtgttct tctccaggtt gacgcagttg gcgcagccgg
     6901 gaccgaggac tttgatgtgc atggtgtgtg actccttctt cgggtgatgg tggtgcttcg
     6961 ggtggtttca gacggggagg acggcgttga acaggtagcc caccgcgatg atcccggcgg
     7021 cgaccacggc gacgaaggtg gcgatcagtt tcggtttgag cacgcggcgc agcaggatca
     7081 tctcgggcag gctgagggcg accgtggcca tcatgaacgc gagcagggtt cccatcggga
     7141 ggcccttgtc gtgcagggcg tcgaccagag gcaggacgcc ggcggcgttg gagtacagcg
     7201 ggacaccgac gacgaccgcg atcaggacgg cgaaggggtt gtcgcggccg gcgtacgtgg
     7261 cgaagaagtt ctccggcgcc cagccgtgga tgaccgcgcc gaggccgatg ccgaccagca
     7321 ggtagggcca tatcttgcgc aggattgtcc cgacctcgtc gatgccgagt tggacgcggt
     7381 cgggccacgt caggtgggcg gtcgagtcga tgacctggcc gcggagccgg gtctcgaaca
     7441 cgaacggttc cacccagcgt tccaggcgga ggcgtccgat cacccagccg gcgacgatcg
     7501 cgatgctgac gcccgcggcg acgtacagca gggtggggcc ggggccgaac aggcccagca
     7561 gcagcgcgat cgccacctcg ttgatcagcg ggctcgcgat caggaagctc atcgtcaccc
     7621 ccaggggaac gccggcggcg acgaacccga tgaacgccgg cacggcgctg cacgagcaga
     7681 acggggtcac gatgcccagc agcgccgcca acacgttccc gacaccctcg cgcttgccac
     7741 cgagcagcgc ccgggtgcgc tccacgctca tgaacgaccg caggaccgtg accacgaaga
     7801 tgatcccggt cagcagcaac gcgatcttga tcgtgtcgta gaagaagaac cacaccccgg
     7861 accccagccg ggactccaga tccaacccag cggcctcgcc gatcagccac gtccagaacg
     7921 gctcgttgac ccagtacaag gccacccacg ccacggacgc ccccgcgacc ccgaggctca
     7981 gccggagccc ggggcgtccc aacctggtcg tcaccgacat gacccacact ccgcccgagt
     8041 agccgcggcc accggcaggg cggcgtgcag ccgctccagg gcgtcctcgg ccagggtgta
     8101 gtccacccat cggccccgcc gggctgtggt caccaggccg gcctcccgca gcatgcgcag
     8161 gtggtagctc agcaggttcg ccgggatcgg cggatcgagt tgcaggtcgc acacgcaccg
     8221 cgtgccggat gccgccaggc ggttgagcag ggtcagccgc accgggtcgg ctgcgaccgc
     8281 cagcagcgtc gcggcatccc gcgtcagttc agcctcaatt gattcaacca cacctgaact
     8341 atgctcccgt gcggccgccc gtgcaagcaa ggcgaccctc atccgtcctg ccacgtcaga
     8401 gccacagccc cgcgagccaa ccggcggccg tggcagtcac cagcagcgtg ccgatgacca
     8461 gcgcgacgat ccgccagcgg gcgatgacga gcatgccgct gatggccccg acgctggtgc
     8521 ccgcgccggt gatgaggaac gcgatgcccg ctccgggggc catgccgccg tccaggaggg
     8581 tggccaccag gggcagcgag gcgtcggtgt tgaggtagac cgggacgccg agcagggcgg
     8641 ccagcgggac cgaccagacg gggttgcccg cgccgagcca cgtggtgagg aggcccgtcg
     8701 gcaggatcct gatgaccagg taccccaggg cggcgaaacc caggaagtac acggcgagcc
     8761 ggcggccatt cgtggccagg gccacgccga accgccgcca ggtggcgggt ttggcgtcga
     8821 ccaccgtggc cggccgggcg tcgatgacgg ccagttgtcg ggtcccgccg acgcgcgtcg
     8881 tgcccggcgc cccggcgacg gcgtccgtct ggcagccggt gtcgcagcca ccgtcgggcg
     8941 tcggggtcgc caggcgggcc tgtccggcga gcagcccgtg gcgctccagc caccaggcgg
     9001 cacctccgcc gatcaggccg acagcgatcg ccgcaccgaa gaacgtggtc gcgaaggcgg
     9061 gcccgaacag cccgaccgac cacacgtact cctgcggact ggtcagcggc gacgacacca
     9121 tgaacgccac caccggcgcc cacgggaccg aggaggccat ggcgcccagc acgacggcgg
     9181 tggtaccaca ggaacagaac ggcgtcagcg cgcccagtgc gacggcgccg aggatcgcca
     9241 cccaggtccg acgccgcagc catctgccca agcggtccgg tttcacgtac acctgcacga
     9301 cggaggccac cacgacactg gcaagcaaga acggccagtt gtgggccagt gcggtcgcca
     9361 cgtcggtcgc gaccccggcg accagggctg tcagatggtc gagcatctac ctgcccaatc
     9421 catagacgag catcgattga tcgatgccga tggatatgat agacacgtgt ctatgagtct
     9481 tcaagaggcg gcgcctcggc ccacgtcggt cggcgatctg tactgttcac cgatcctgca
     9541 ggcccccctg cagcccgacg atgccttggc cctggccggg gcactcaagg tgctggccga
     9601 cccggcgcgg ttgcggctgc tgagcctgat ccgagccgcg gacggcgggc gggcgaccac
     9661 ccgagtcctg gccggtcggc tgggattgac ccagccgacc gtcacccatc atctgggtgc
     9721 cctgttcgac gccggcttcc tcgaccggga gcgggtgggc cgacagacct ggtactcggt
     9781 gaacaccgat gggctgcggg cgatccaaca gctccttgcc ccgacacctg tgggaactgt
     9841 cgagtcgtga ggtaggtaag cgacagcgtg cagccgcgat cggaccgcgg cgaagggagc
     9901 tgcggacacc tcttcttcaa gtgggccgat cgggctgacg cggagtgcgt tcagctggtc
     9961 agttctgtcg cgagtgcgtg cacccggttg gcgatctggt cgcggatgtt gcgggtccgt
    10021 tcgtcgccct cgatgccgtc ctcggccggg tcgtggatcg gccagcgctc gacgcggtcg
    10081 gccagcggct cgggtgggtt cagctggacg tcggggccga tgaggatgat gcggtcggct
    10141 gcgtccagca tggcggcggt cacggccttg gggttctccc cggcggtgct ggcgcccagc
    10201 tcggcgacga tgcgcgccga cgcgtcgtcg acggcgtcgt cgggtccggt gccggcagtg
    10261 gtcaccacga tccggtcgcc gtacaggtgg cgcatcaggg ccccggccat ctgggacttg
    10321 cccgcgttgt gcacgcagag gaacagcaca tgggggaggt ggcttggctt gatcgtgctc
    10381 gggagggagg tcgccctctg cagctggcgg gtgtgggtct ggtgggtgct gttgccgccc
    10441 gacaggttca tcacctcagc gaggccgagg gcgagcagga cgttctgggc ggcgttcccg
    10501 gtcacgccct tgttgcagta cgtgaccgtc ggcacctgtg ggtcgagttc gccgacgcgc
    10561 cgccgcaggt ctgccagcgg gatgttcacc gccccgggca ggtggctctt ggcgtagtcc
    10621 ttcgcggcgc gcacgtcgac gatctggatc cgctcgcctc gcgccagccg ctcatcgagg
    10681 tcggtgggcg atatcagtgg agctcgtccg cggacggcgg tctcgagcgc catacccgtg
    10741 tagtggaccg ggtccttcgt ggtggcgaag ggcggtgagt aggcgaggtc gaggtggaac
    10801 aggtcggcca ctgtggcgcc gaaggtgatc gcggtggcga gcacgtcgat gcgcttgtcc
    10861 actccctgtg gtccgacggc ctgggcgccc aggagtcggc cggaggcgcg gtcggccagg
    10921 gccttgatgg tgagttcctt gccgcccagg taggtcgcgt gggccggttt ggtgttgtgc
    10981 aggacgaccg ggtcgaatcc ctcggcgcgg gcctgcgtct cggtgagccc ggtgtggccg
    11041 acggccagtt cgaacacccg cacgatgccg gtgcccagga tcccgcgatg ctccagggtg
    11101 gtgcctccgg tgatctggtc gccggcgatt cgccccatct tgttcgccgt cgatcccagc
    11161 ggccgccaca acggccggcc cgtgatcacc gagtagcttt cggcacagtc accgaccgcg
    11221 aagacgccct cgacgttggt gcgcatggac gggtccacgg cgatcgcgcc ggtctcgccg
    11281 atggtcactc cgaggtcgcg cgcgaggtcg gtgttgggcc gcacaccgac ggcaaggatc
    11341 accagctgcg ccgcaatgag ttcgccggtg tcgagccgca cgccgcagac ccgggtgtct
    11401 ccttcgagtc cggtcatgga cgtgttgagc cgtacggtca cgccgtggcg ctcgagttcc
    11461 tcctggacgc ggaacgccat gtccgcgtcg agggccggca tgacctgcgg gaggcgctcg
    11521 accagggtga cgccgatccc gctcgcgacc agctgttcgg ccacctccag gccgatgaag
    11581 ccggatccga cgaccacgac ctgctccggt gaggcgtcgt cgagccagcc cttgatcgcc
    11641 tcggcgtcgc ggacgctgcg caaggcgaag actcccggca ggtcgacccc cggcaccggc
    11701 gggatgatgc tggtggcccc ggtggccagc acgaggctgt cgtagcggtc cgcggtcacc
    11761 tcgccggagg acaggtcgcg aacctggagg gtccggccgg ccgggtcagc gctgatcacc
    11821 tcgtgaccgg tgcggatgtc gatgttgtag cgcttggcga accaggccgc gtcgcggggg
    11881 tgcaggtcgt cggcgtcggg cacggtcccg ccgatgaagt acggcaggcc gcacccggag
    11941 taggagatgt cacggtcccg ctcgtagatc acgatctcca tctcctcgtc gttgcgccgg
    12001 gccttggcgc ccaccgacgt gccggcggcg accgcaccga cgatcacgag ccgctgcgcg
    12061 cgcggcgacg ctggcatggg catgtcggac acgggaacct cctgggttga tgccccgcgg
    12121 gtgcgggact tcgtcggatc gaagtcgagc gtcgaagccg gatgggccgg ccttgactcc
    12181 aacaatagct cagctagcat tgaggcaata cctgtcgagg taatcagtcg aggtaatcat
    12241 ctgaggaagg ggccgtcgtg gtactcgggg acgtgaacgc cagggcgcgc gcgtacgccg
    12301 cactgggtga cccgtcgcgg ctcgcgatca tcgacctgct cgcgcacgcc gacctcgcct
    12361 caggcgagct cgccgcccag ctggagttgc cgaccaacct ggtcgcccac cacctcggcg
    12421 tccttgagca ggcgggcctc gtcacgcggc accgttccga gggagacgga cgccgcagct
    12481 acgttcagcg gaccccggcc tgcgaccggt tgctcggtgc gaccccgctg cctcgtccgg
    12541 cccgggtcgc tttcgtgtgc agccagaact cggcccgttc acagctggcg gagtcctact
    12601 ggcgctcgat cagcgacatt cccgccctct cggcaggaac ccgccccgcc cgacgggtgc
    12661 acccgggcgc ggtctcggtc gcgcgccgcc acgggctcga cctcacgcac gcgacaccca
    12721 agctcgcccg ggacgtcctc accggcgacg agctgctcat cgcggtgtgt gaccgggcct
    12781 acgaagacgt ggacggcccg ctgctgcact ggtccattcc cgacccggca gtcaaggagg
    12841 acccccatgc cttcgacgcc gcctacgacg acatcaccca acgcatcgac cgactcgcat
    12901 caagccttga aggagagcca cgatgaccgt ccaccctgct caggaacgca gctgggaggg
    12961 tctgcacctt gacgacagac tcgcgctcaa gcaggctgcg gcgcacctcg cgcaggagtt
    13021 ctccggcctg tatggcactc agaccatcga gcggttcatg gacagttcct tcgaccagtt
    13081 cgccggccgc gcgaccgtca ccaagttcct gcccctgctc gccgaacgct tcgccaggca
    13141 gcgcctccac gcgctcgccc gcgtcgaggg gctccacgac gacggcaaac ccgtggtgct
    13201 cttcctgtgc acccacaacg ccggacggtc ccagatggct ctcggcttct tccaggcgct
    13261 cgccggcgac aatgcggtcg cctggtccgg cggctccgag cccgggctcg aggtcaaccc
    13321 cgtggcggtc caggcgatgg ccgaacgcgg aatcgatatc gcccacgagt tccccaaacc
    13381 ctggaccgat gagaccgtcc gcgccgccga cgtcgtcatc accatgggct gcggcgacgc
    13441 gtgccccatc ttcccgggca agcgctacga aagctgggaa ctcgacgacc cccacggcca
    13501 ggatctcccc gccgtccgac ccatccgcga cgacgtcgaa cgacgcgtcc gcagccttct
    13561 ggcatcgctc aacctgtgac ggaggg
//
LOCUS       DAOSUG010000012        13236 bp    DNA     linear   ENV 10-JUN-2024
DEFINITION  MAG TPA_asm: Micropruina sp. isolate AMR_MDS_2195
            NODE_2783_length_13236_cov_4.643047, whole genome shotgun sequence.
ACCESSION   DAOSUG010000012 DAOSUG010000000
VERSION     DAOSUG010000012.1
DBLINK      BioProject: PRJNA925477
            BioSample: SAMN32864262
            Sequence Read Archive: SRR4236673
KEYWORDS    WGS; ENV; Metagenome Assembled Genome; MAG; Third Party Data; TPA;
            TPA:assembly.
SOURCE      Micropruina sp. (wastewater metagenome)
  ORGANISM  Micropruina sp.
            Bacteria; Bacillati; Actinomycetota; Actinomycetes;
            Propionibacteriales; Nocardioidaceae; Micropruina.
REFERENCE   1  (bases 1 to 13236)
  AUTHORS   Abdulkadir,N., Saraiva,J.P., Zhang,J., Stolte,S., Gillor,O.,
            Harms,H. and Rocha,U.
  TITLE     Genome-centric analyses of 165 metagenomes show that mobile genetic
            elements are crucial for the transmission of antimicrobial
            resistance genes to pathogens in activated sludge and wastewater
  JOURNAL   Microbiol Spectr 12 (3), e0291823 (2024)
   PUBMED   38289113
REFERENCE   2  (bases 1 to 13236)
  AUTHORS   Abdulkadir,N.
  TITLE     Direct Submission
  JOURNAL   Submitted (24-MAR-2023) Environmental Microbiology, Helmholtz
            Center for Environmental Research -UFZ, Permoserstr 15, Leipzig
            04318, Germany
COMMENT     The annotation was added by the NCBI Prokaryotic Genome Annotation
            Pipeline (PGAP). Information about PGAP can be found here:
            https://www.ncbi.nlm.nih.gov/genome/annotation_prok/
            
            ##Genome-Assembly-Data-START##
            Assembly Method        :: SPAdes v. 3.11.1
            Genome Representation  :: Full
            Expected Final Version :: Yes
            Genome Coverage        :: Not Applicable
            Sequencing Technology  :: Illumina MiSeq
            ##Genome-Assembly-Data-END##
            
            ##Genome-Annotation-Data-START##
            Annotation Provider               :: NCBI
            Annotation Date                   :: 05/04/2023 04:57:10
            Annotation Pipeline               :: NCBI Prokaryotic Genome
                                                 Annotation Pipeline (PGAP)
            Annotation Method                 :: Best-placed reference protein
                                                 set; GeneMarkS-2+
            Annotation Software revision      :: 6.5
            Features Annotated                :: Gene; CDS; rRNA; tRNA; ncRNA
            Genes (total)                     :: 2,776
            CDSs (total)                      :: 2,750
            Genes (coding)                    :: 2,714
            CDSs (with protein)               :: 2,714
            Genes (RNA)                       :: 26
            tRNAs                             :: 24
            ncRNAs                            :: 2
            Pseudo Genes (total)              :: 36
            CDSs (without protein)            :: 36
            Pseudo Genes (ambiguous residues) :: 0 of 36
            Pseudo Genes (frameshifted)       :: 2 of 36
            Pseudo Genes (incomplete)         :: 32 of 36
            Pseudo Genes (internal stop)      :: 2 of 36
            ##Genome-Annotation-Data-END##
FEATURES             Location/Qualifiers
     source          1..13236
                     /organism="Micropruina sp."
                     /mol_type="genomic DNA"
                     /submitter_seqid="NODE_2783_length_13236_cov_4.643047"
                     /isolate="AMR_MDS_2195"
                     /isolation_source="Activated sludge of municipal
                     wastewater treatment plants"
                     /db_xref="taxon:2737536"
                     /environmental_sample
                     /geo_loc_name="Canada"
                     /lat_lon="49.25 N 123.1 W"
                     /collection_date="2015-06-16"
                     /metagenome_source="wastewater metagenome"
                     /note="metagenomic"
     gene            <1..1619
                     /locus_tag="PLF56_00815"
     CDS             <1..1619
                     /locus_tag="PLF56_00815"
                     /inference="COORDINATES: ab initio
                     prediction:GeneMarkS-2+"
                     /note="Derived by automated computational analysis using
                     gene prediction method: GeneMarkS-2+."
                     /codon_start=3
                     /transl_table=11
                     /product="hypothetical protein"
                     /protein_id="HOQ52133.1"
                     /translation="ERVDRLIASNLQERTLVWIPRFFSSERMRDVRRLVILNWLLDGS
                     GDRFAGYADHLSEADRGIARAILESQRTTLKHELERAIGVAYDAASPTRSDDVQTDAA
                     HDRVINSLVRWFTPQPPVAATLGGAFASLVQQAFDAYYPAHPHFEPADKEITRRELAV
                     VLAHVSRALATPDGRVPLEGDINAVRRIANPLRVGWAAETHFLMGDDRFNEWGPAFER
                     ALGARGGDLSAPVTVSEARSWIDALEPKRGLKPEVADLVILAWAALRRRAWFLRGAAI
                     AAPSPGELRPEMELREQPMPSAAEWKTATEMAGLLFGVSAGAYLTPAEVASFAEKVKA
                     AAQDRYQPAYRLQAALEESQQRLGLGEQGESVRLATARATAALADRLRQLGGVELIRA
                     LAGAKIAEPARAGRSLTSADAVVGVLKRFEWDRLEPLRRAAEGEGRSADRASEIIRAF
                     EAAVNDDELTQAIAPAIQRADREVFDWLRDQVATPPPPPPPPPPPPPPVPRGRRTLRP
                     GESLGAVTAELEAFRTDHAGRPVTVEWRAE"
     gene            1616..4288
                     /gene="pglZ"
                     /locus_tag="PLF56_00820"
     CDS             1616..4288
                     /gene="pglZ"
                     /locus_tag="PLF56_00820"
                     /inference="COORDINATES: protein motif:HMM:NF033446.0"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="BREX-2 system phosphatase PglZ"
                     /protein_id="HOQ52134.1"
                     /translation="MSAAPATAVTPAMVRALIDEAKRKNYDHGVLGFRSTAQGAEPQT
                     FQHDGQTVAVVPCVSALAARAALAERPADGWLVLVTDRSDDDLGAGLLAHLVGQRFRS
                     PDAWQAVQQRFAATGIDTHLAGGPGHKDIAAGLLEATPPEGWPPAPAGLLSRDHAFGA
                     VARRWLDVPDGPLDLLGVLDWSTKRTGTARIGDLRRLAGDALTDAVLAWISASTGPAA
                     PLVARLVEGGAVSDLLPLGLVLDPLLADPTTDAALVIARLQHRWGAVPPSALSALAAG
                     ATAVAGDLLADDARREEVARVLARADEFAREGQGEPLTIRSAVLRSGLVQRLRRLADE
                     LRRDPTDRLPAIESAWALVAEHRLRGFEASVPALLAAVRLVRWLAGVASEDPPRALAA
                     LARRQADVDAWVDSAVNDATTGVEDERVAAALEDVLARVKLVRDEHDRQFARALAETS
                     GGASVASSLGDADAPVWLLEHVLADVVADVARKQPTLLLVLDGLSTGVATEVVADIDQ
                     RTDVAMTEALLPRLRRRGAALAVLPSITDASRTSLLCGTLTTGQQATELAGYQRLVSG
                     LGLGESPLFHKRILDTSRPGFAVSDEVKTAIADTSKKLVTCVLNTIDDALDRSDPGGT
                     EWTADTVKHLVPLLQRAREAGRVVVIASDHGHVVERRAGAQRSYPGGANTRYRAAGGE
                     VGDDEVLVTGPRVLTPDHRAVLAVSERLRYGPLKAGYHGGAAPAEVVVPVVLLVPSGV
                     DEERWRPAPYQAPSWWEGPSAAVAPPLPTAPEVAQPDLFTEPAPAATRVGAALVASST
                     YADQRRLAGRVVVTDTAVVVLMDALASSPSRRLTAGQAATALGVPVTRAQLALGQVAK
                     LINVEGYPVLSIDPATGAVVLDERMLTEQYGLGR"
     gene            4369..4710
                     /locus_tag="PLF56_00825"
     CDS             4369..4710
                     /locus_tag="PLF56_00825"
                     /inference="COORDINATES: protein motif:HMM:NF024949.2"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_function: GO:0003677 - DNA binding [Evidence IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="helix-turn-helix transcriptional regulator"
                     /protein_id="HOQ52135.1"
                     /translation="MNATVADRVRQRIAAQGRTQSDVASSVGLSDSQLSKSISGARQF
                     SAVEVASLASALGVSMHWLVTGEEDPLGWRLAARHTFDPATGRYGADFLGEDKAILQD
                     VALLYRQAHHR"
     gene            4782..5450
                     /locus_tag="PLF56_00830"
     CDS             4782..5450
                     /locus_tag="PLF56_00830"
                     /inference="COORDINATES: protein motif:HMM:NF017885.2"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="ImmA/IrrE family metallo-endopeptidase"
                     /protein_id="HOQ52136.1"
                     /translation="MPGRITGRLDVDVWVLDLTGDAYSIVADGRRVIVAKRTPNWFRQ
                     NFSIAHELGHLAADSLCDESLGDAGDHEAAANAFAAEFLMPEAELRSFDWQRLSLPVL
                     AEHLWEWGISTQALSVRLRSLGLTPGVETAAALTLNTQAFLRRHWVWSPGPDPITLRM
                     ELAAERRVPTELTSLLEERVAQGLAPVESLAYALGVPASEVEVELPSLADPDLDRDLL
                     EGLD"
     gene            5453..6037
                     /locus_tag="PLF56_00835"
     CDS             5453..6037
                     /locus_tag="PLF56_00835"
                     /inference="COORDINATES: ab initio
                     prediction:GeneMarkS-2+"
                     /note="Derived by automated computational analysis using
                     gene prediction method: GeneMarkS-2+."
                     /codon_start=1
                     /transl_table=11
                     /product="hypothetical protein"
                     /protein_id="HOQ52137.1"
                     /translation="MPTFFFPDNTVLINFTLLDRHDLLEWFVRGSGQWTLSVSRECHR
                     SSQQPGLSRLADWSDVFHEPLIPEPRELVDARTIAEHMRKPGESEPSQHMGEAETIAI
                     VVNRGLSTVFLTDDHDAARRAAAEPLITVASTTKIIAFAEVKGRLEHAEARSYLAGLL
                     NARRVLGNPPLVADYDSYVASLRNAVPFSGPPPR"
     gene            6047..7375
                     /gene="brxD"
                     /locus_tag="PLF56_00840"
     CDS             6047..7375
                     /gene="brxD"
                     /locus_tag="PLF56_00840"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_013863742.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="BREX system ATP-binding protein BrxD"
                     /protein_id="HOQ52138.1"
                     /translation="MTEVSPRRRREVIDALRRGTVPANGLDLLAVGLDRFEPALRSEL
                     DAVAGGGAVFKAVRGEYGSGKTFFSRYLAEAVLRQGWASAEVQVSETETPLHRLETVY
                     RRITESLRTATVPPSAFRATLDGWLFTLEDDALSADTSLAGASEHVLATAVEALLDQR
                     LAGVSSNTPAFAQALRGYRAAVLAGDGPAADALAAWLGGQPHVASAAKRAAGVRGDLD
                     HFGAMSFLQGLLTVLRDAGHPGLLLVLDEVETLQRVRGDVRDKALNALRQLIDEIDAG
                     RYPGLYLVITGTPAFFDGPSGVPRLPPLAQRLHTDFTTDARFDNPRAVQVRLPGFDRA
                     ALLAVGQRVRDLYADGTDNAVRVRSMVDDAYVASLADAVTGSLGGKVGIAPRVFLKKL
                     VADVLDRVDQFADFDPRQHYALTIGDGDLTEVERNAARPPARADGVALEL"
     gene            7392..9527
                     /locus_tag="PLF56_00845"
     CDS             7392..9527
                     /locus_tag="PLF56_00845"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_013863741.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_function: GO:0003676 - nucleic acid binding [Evidence
                     IEA];
                     GO_function: GO:0005524 - ATP binding [Evidence IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="DEAD/DEAH box helicase"
                     /protein_id="HOQ52139.1"
                     /translation="MTGFDLLHPVVQHHVVNTLGWSALRPLQDSAVEPVLAGSDALLL
                     APTAGGKTEAALFPLLSRMANEDSRGTSVLYLAPLRALLNNLEPRVRGYARWLGRDAS
                     VRHGDTSRGVRRRQAVERPDVLLTTPESLESILVSATVDPRVVLADVRAVIVDEVHAF
                     AGDDRGWHLLAVLERLTRIAGRPLQRIGLSATVGNARELLTWLQGSSAGVRPGVVVAP
                     HAGGGPVPELELDYVGSVDNASVVIASLHLGEKRLVFADSRRVVERLAGRLHDAGVET
                     FVSHSSLSAVERRRAEAAFAESRDCVIVSTSTLELGIDVGDLDRVVQIGAPGSVASLL
                     QRLGRTGRRPGTVRNILMLATDDTELLRGFGLALLWSEGYVEPIRPTPTPQHVAAQQL
                     LALALQEGRVGRLTWPEWLGGLALASPRALGVIADWLVETGHLDVDTDMLFVGPEAER
                     KYGRRHFMELMTVFTTDPQVTVLHGRDEIGVVDPMVLLSKVTGPRVIALGGRSWKVTG
                     MDWERRRAYVEPAPGPGLARWAGTGAPPRSFALTGAMRRVLLGHDPAGAMITKRAEAR
                     IGELRVQWDARVDPTRSVVMRDSEGLRWWTWAGSRGNAVLHAALQAVAPQLLDPIETF
                     DNERITLHPDARAHDLSGALREARVRFGSDLAEVHPRVDRRAVEGLKFADLLPPELAE
                     GTLAARIGDVDGARRVLSLGVVERIVSSI"
     gene            complement(9796..10191)
                     /locus_tag="PLF56_00850"
     CDS             complement(9796..10191)
                     /locus_tag="PLF56_00850"
                     /inference="COORDINATES: ab initio
                     prediction:GeneMarkS-2+"
                     /note="Derived by automated computational analysis using
                     gene prediction method: GeneMarkS-2+."
                     /codon_start=1
                     /transl_table=11
                     /product="hypothetical protein"
                     /protein_id="HOQ52140.1"
                     /translation="MNGNDVMFTAALSLLGAFIGLAIRSRLADLAYRRDGEVDQPYPG
                     PRWWIPVATAAASGLLGWRFGVDRWPLLLPTLSLAWFGPWLSAIDLDVRRLPNRLLAV
                     HGALVALGVGAAAILTGDLSIAIQPPSVR"
     gene            complement(10262..10945)
                     /locus_tag="PLF56_00855"
     CDS             complement(10262..10945)
                     /locus_tag="PLF56_00855"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_015071525.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="vWA domain-containing protein"
                     /protein_id="HOQ52141.1"
                     /translation="MTDPNRTHLYFLLDRSGSMQSIRDDTIGGFDAFMAEQRQHAGDC
                     RVTLAQFDDRYEVVYTDRPIAEVPGLDLQPRGTTALLDAIGRLVTDAGAHLASLPECQ
                     RPGTVIVGIMTDGYENASREWTHPAIKALVQQQTKTYNWEFLYMGADQDAIEVGASLG
                     VQANRSMTYSRDASGPAMAATSAVVGRLRAARATGAPAADIGYSAEERQASAGAPSGR
                     PPKKTRPTR"
     gene            complement(10966..11361)
                     /locus_tag="PLF56_00860"
     CDS             complement(10966..11361)
                     /locus_tag="PLF56_00860"
                     /inference="COORDINATES: ab initio
                     prediction:GeneMarkS-2+"
                     /note="Derived by automated computational analysis using
                     gene prediction method: GeneMarkS-2+."
                     /codon_start=1
                     /transl_table=11
                     /product="hypothetical protein"
                     /protein_id="HOQ52142.1"
                     /translation="MGTGRDELDALISDAAAALTHAAGATTAKDALPLLSEAQEQLTR
                     AIDEAMAAAVLEEGTSLRQAGQLASLSENAVGPRLARTARLMPYATPEGRVTRTSVER
                     ARYDAEIGRPAPTPEHPIEPLRFRRRRDT"
     gene            complement(11956..>13236)
                     /locus_tag="PLF56_00865"
     CDS             complement(11956..>13236)
                     /locus_tag="PLF56_00865"
                     /inference="COORDINATES: ab initio
                     prediction:GeneMarkS-2+"
                     /note="Derived by automated computational analysis using
                     gene prediction method: GeneMarkS-2+."
                     /codon_start=1
                     /transl_table=11
                     /product="hypothetical protein"
                     /protein_id="HOQ52143.1"
                     /translation="GWNLGTLASGSKFAAQVRWLPATAATTASVVAAMGPAISLMVIQ
                     AQLNQIARLAEHNLELTSTVLEVVRRDQWSSVTGHYKTLMREFGHARQIGEVTDAIYQ
                     EVRGYEGKLTSQWDVFKSAVQQHVSALRTKRGHQTRQKYLLDNGRAIVADAQALLLAQ
                     TSWFTHQAMRAAHLSRSAGSNPQDERLLKNLIAEVQDLHEKTLDETNWLLDQLAREFA
                     IIAELPGKKTFKIGATARAAKDAAKMVRQLQEALASVRGQDAPKEPQPLALPSILVFE
                     DKVPGELVRILPLRLELDERVLALADASCDRWKFLGGGWVAITDRRLLITKQDSLRQV
                     GGIDISIGTGDIRYVRRHNPVNKPPVVDVITKNTSLTLHFPAWAKNDGARRDAGRLGE
                     LLASYMNLPESEVPTVRPLELTSVAGEQIEATEA"
BASE COUNT         1950 a         4365 c         4759 g         2162 t
ORIGIN      
        1 tggaacgtgt cgaccgcctg atcgcgtcga acctgcaaga gcggacgctg gtctggatcc
       61 cgcggttctt ctcgagcgag cgcatgcggg acgtccggcg gttggtcatc ctgaactggc
      121 tgctggacgg cagcggcgac cggttcgccg gctatgccga ccacctgtcg gaggccgacc
      181 gggggatcgc gcgggccatc ctcgagtcgc agcgcacgac gctgaagcac gaactcgagc
      241 gagcgatcgg cgtcgcgtac gacgcggcct cgccgacgcg gagcgacgac gtccagaccg
      301 atgcggcgca cgaccgggtg atcaactcat tggtgcggtg gttcacgccc cagccgccgg
      361 tcgcggccac gctgggcggc gccttcgcgt cgctggtgca gcaggccttc gacgcgtact
      421 acccggcgca ccctcacttc gagcccgccg acaaggagat cacccgacgc gagctggccg
      481 tcgtgctggc ccatgtgagt cgggcgctgg ccacgccgga cgggcgcgtc ccgctggagg
      541 gcgacatcaa cgccgtgcgg cggatcgcca acccgctgcg ggtcggctgg gccgcggaga
      601 cccacttcct gatgggcgac gatcgcttca acgagtgggg cccggcgttc gagcgagcac
      661 tcggcgcccg cgggggtgac ctgtcggctc cggtgaccgt gtccgaggcg cggtcctgga
      721 tcgacgcgtt ggagcccaag cggggactca agcccgaggt ggctgacctg gtgatcctgg
      781 cgtgggcggc gctacggcgt cgtgcctggt tcctgcgcgg cgcggcgatc gcggcgccct
      841 cgccgggcga gctgcgcccg gagatggagc tgcgcgagca accgatgccc tcggccgcag
      901 agtggaagac ggccacagag atggccgggc tccttttcgg ggtcagcgcg ggggcctacc
      961 tgacgccggc ggaggtggcg tcgttcgccg agaaggtgaa ggcggccgcc caagatcggt
     1021 accagcccgc ctaccggctt caggccgcgc tggaagagtc gcaacagcgg ctgggtcttg
     1081 gagagcaagg ggagtcggtg cgcctcgcga ccgcgcgcgc cacggcggct ctggccgacc
     1141 ggctgcgtca gctcggcggc gtcgagctca tccgggcgct ggcgggggcg aagatcgctg
     1201 agcccgcccg cgccggacgc tcgctgacga gcgcggacgc cgtggtcggg gtcctcaaga
     1261 ggttcgagtg ggatcggctg gaacccctcc gacgtgccgc cgagggcgag ggccggtcgg
     1321 ccgaccgagc gtccgagatc atccgggcgt tcgaggccgc cgtgaacgac gacgagctca
     1381 ctcaggcgat cgctcccgcc atccagcgcg ccgacaggga ggtcttcgac tggctgcgtg
     1441 atcaggtggc cacgccgccg cccccaccac cgccgccgcc cccaccaccg cctccggtgc
     1501 cacggggcag gcggacgctg cgccccggcg agagcctggg ggcggtgacc gcggaattgg
     1561 aggccttccg gaccgaccac gccggacgcc cggtgaccgt tgaatggcgg gcggaatgag
     1621 cgctgccccc gcgacggcgg tcacccccgc gatggtgcgc gccctgatcg acgaggcgaa
     1681 gcggaagaac tacgaccacg gcgtcctcgg attccgatcc accgcccagg gagcagagcc
     1741 ccagacgttc cagcacgacg ggcagaccgt ggcggtcgtg ccgtgcgtgt cggccctggc
     1801 tgcgcgggcg gcgctggcgg agcggcccgc tgacggctgg ctcgtgctgg tgaccgatcg
     1861 cagtgacgac gacctcggtg ccgggctgtt ggcccacctg gtcggacagc ggtttcgatc
     1921 gccggacgcg tggcaggccg tccaacagcg attcgctgcg accgggatcg acacccatct
     1981 tgcgggcggg cccggccaca aggacatcgc cgctggactg ctggaagcga ccccaccgga
     2041 agggtggcct ccggcgccgg ctgggctgct gagccgcgac cacgccttcg gcgcggtggc
     2101 gcgacgttgg ctggacgtcc ccgacggacc gctcgacctc ctgggcgtcc tcgactggtc
     2161 gacgaagcgg acgggaacgg cgcgcatcgg ggatctgcgg cgcctggccg gcgacgcgct
     2221 gacggacgcg gtactcgcgt ggatctccgc gtcgacgggg cctgccgcgc cgctggtggc
     2281 ccggctcgtc gaggggggcg ccgtctccga cctgctgccc ctcggcctgg tgctcgaccc
     2341 gctgctcgcc gacccaacca cggacgccgc cctcgtgatc gcccgcctac aacaccggtg
     2401 gggggctgtg ccgcccagtg cgttgagcgc tctcgccgcc ggagccaccg ccgtcgcggg
     2461 agacctgctg gctgacgacg ctcgccgcga ggaagtcgcg cgagtcctcg cgcgtgcaga
     2521 cgagttcgcc cgtgagggac agggtgaacc cctgacgatc cgttcggccg tgctgaggtc
     2581 cggtctggtc cagcggctgc gccgactggc cgacgagctt cgccgcgatc ccacggatcg
     2641 gctccccgcc atcgagtcgg cctgggcctt ggtcgccgag catcggcttc gtggcttcga
     2701 ggcgagtgtg ccggccctgc tggcggcggt tcgcctcgtg aggtggttgg ccggagtggc
     2761 gtccgaggat ccgccgaggg cgttggcggc tctggctcgc cggcaggccg acgtggatgc
     2821 ctgggtggat tcggcggtca acgacgcgac gacgggcgtc gaagacgagc gggtcgctgc
     2881 cgcgctggag gacgtcctgg cccgggtgaa gctggtgcgg gacgagcacg accggcagtt
     2941 cgcgcgcgca ctcgccgaga cgtccggcgg ggcatctgtt gcgagcagtc tgggggacgc
     3001 cgacgcgccc gtctggctgc tggagcacgt gctggcggac gtggtcgcgg atgtggcacg
     3061 gaagcagccg acgctgctgc tggtgctgga cggactgagc accggggtcg ccaccgaggt
     3121 ggtggccgac atcgaccagc gcaccgacgt cgcgatgacc gaggcgctcc tgccccggct
     3181 gcggcgtcgc ggggccgcgc tggccgtcct cccgtccatc accgacgcga gccggacgtc
     3241 gctgctgtgc ggaacgctca cgaccggcca gcaggccact gagctcgccg gttaccagcg
     3301 cctcgtctcc gggctgggtc ttggggagtc gcccctgttc cacaagcgga tcctggacac
     3361 gtcgcgtccg ggcttcgcgg tgtccgacga ggtcaagaca gcgatcgccg acacctcgaa
     3421 gaagctggtc acgtgcgtgc tcaacacgat cgacgacgcc ctcgacagga gcgacccggg
     3481 aggcaccgaa tggacagccg acacggtcaa gcacctggtg cccctgctcc agcgtgcccg
     3541 cgaggccggt cgcgtcgtcg tcatcgcatc cgatcacggg catgtcgtgg agcgtcgggc
     3601 cggcgcccag cgctcctacc cgggcggagc caacacgcgt taccgcgccg ctggcggtga
     3661 ggtcggcgac gatgaggtgt tggtgacagg gccgcgggtt ctgacgccgg atcaccgtgc
     3721 cgtgctggct gtgtcggagc ggctgcggta tgggcccctc aaggccggct accacggtgg
     3781 cgcggcgccg gccgaagtcg tggttccggt cgtgttgctg gtcccctccg gggtggatga
     3841 ggagcgttgg cgtccggccc cctatcaggc cccgtcatgg tgggagggcc cgtcggctgc
     3901 ggttgcgccg cccctcccga ccgcgcccga agtcgcccag cctgacctgt tcacggagcc
     3961 ggcgccggca gccacccgcg tgggcgctgc cctggtcgcg tcgtcaacgt acgcagatca
     4021 gcggcggctt gctgggcgcg tcgttgtcac cgataccgcg gtggtggtgt tgatggacgc
     4081 gttggcgtct tcgccgagcc gacggctgac agcggggcag gcggccacgg cgctgggtgt
     4141 cccggtcacg cgggcgcagc tcgccctggg tcaggtggcg aagctgatca acgtcgaggg
     4201 ctatcccgtg ctttccatcg accccgccac aggcgccgtc gtgctggacg agaggatgct
     4261 gacagagcag tacgggctgg gccggtgacg gctgaggtag ccgcgtcgta gagaaaacgt
     4321 gggctatgaa ggtttcccat ttcgaaaaac gaggcgtaca gtcgtcgtat gaacgcaacg
     4381 gttgctgatc gcgtccgtca gcgcatcgcg gctcagggac gcacccagtc tgacgtggcg
     4441 tcgagtgtcg gactttccga ctcccagctg tcgaagtcga tctcgggcgc tcgccagttc
     4501 tcagcggtgg aggtagccag cttggcctct gcgctcggag tgtccatgca ttggctggtc
     4561 acgggcgaag aggatcctct gggctggcgg ctagccgcgc gccacacgtt cgaccccgcc
     4621 acgggtcggt acggggcgga cttcctcggc gaggacaagg cgattctcca ggacgtggcg
     4681 ctgctttacc ggcaggctca tcacagatga gtcgagcttc ggtcgcgggt ctgggtgccg
     4741 cgggagtgcg acgcctgttg ggtgacggat tcacccatga cttgcccggc cgcatcacgg
     4801 gaaggctcga cgtcgacgtg tgggtgctcg acctcacggg ggacgcgtac tccatcgtgg
     4861 ccgacggccg tcgggtgatc gtcgccaagc gcaccccgaa ctggttccga cagaacttct
     4921 ccatcgctca cgaactggga cacctcgcgg cggacagcct gtgcgacgag tccctgggtg
     4981 acgccggcga ccacgaggcc gctgctaacg cctttgccgc agagttcctg atgcccgagg
     5041 ctgaactgcg gtcgttcgac tggcagaggc tcagcctccc ggtgctggcc gagcaccttt
     5101 gggagtgggg catttcgacg caggcactgt cggtgcgtct gcgatcgttg ggcctcacac
     5161 ccggtgtcga gaccgccgcc gcactcaccc tcaacaccca agcgttcctt cgacgccact
     5221 gggtctggtc gccgggccca gatcccatca cgctgcgcat ggagctggcg gcggagcgaa
     5281 gggtccccac tgaactgacg agcctcctcg aggaacgggt tgcacagggc ttggcgcccg
     5341 tcgaatccct ggcgtacgcg cttggggtgc cagcgagtga agtggaagtc gaactgccgt
     5401 cgctggccga cccggatctc gatcgcgacc tgctcgaagg tctcgactga ccgtgccaac
     5461 gttctttttc cccgacaaca cggtcctgat caacttcacc ctgctcgacc ggcatgacct
     5521 cttggagtgg ttcgtcagag gctcgggcca gtggacgctc agcgtttctc gggaatgcca
     5581 cagaagttct cagcagccgg ggctgtcccg ccttgccgac tggtccgacg tgttccacga
     5641 accactcatc ccggaaccca gggagttggt ggacgctcga acgatcgccg agcacatgag
     5701 gaagccgggc gagtccgagc ccagccagca catgggggaa gccgagacga tcgccatcgt
     5761 cgtgaaccgc ggattgtcga cggtgtttct caccgatgac cacgatgccg cgcgtcgagc
     5821 ggcggcggag ccgctgatca cggtggcgag caccaccaag atcatcgcgt tcgccgaggt
     5881 caagggacga ctcgagcacg cggaggcgcg gagctatctc gcgggactgt tgaatgccag
     5941 gcgagtcctg ggcaacccac cgttggtagc cgactatgac agctacgttg cgagcctccg
     6001 gaacgccgtg ccattcagcg gtccgccccc tcgatagggt gccacgatga ccgaggtttc
     6061 tccgcgccgg cgacgtgagg tgatcgacgc gttgcgacgt gggacggtgc ccgccaatgg
     6121 cctcgatctc ctagccgtcg gcctcgaccg cttcgagcct gcgctgcgtt ccgagctcga
     6181 cgccgtcgcg ggcggcgggg cagtgttcaa ggcggtccgg ggcgagtatg gctcgggcaa
     6241 gacgttcttc tccaggtatc tcgccgaggc cgtccttcgc caaggctggg cgtccgcgga
     6301 ggttcaagtc agcgagacgg agactccgtt gcaccgactg gagacggtgt atcggaggat
     6361 caccgagtcg ttgcggacgg ccacggtgcc gccgagcgcg ttccgcgcga ccctggatgg
     6421 ctggctgttt accctggagg acgacgcgct cagcgccgac acgtccttgg ctggcgcctc
     6481 ggagcacgtt ctggctaccg cggtggaggc cctgctcgac cagcggctgg cgggagtcag
     6541 cagcaacacg ccggcgttcg cgcaggcgct gcgcggctat cgtgccgccg tgttggctgg
     6601 ggatgggccc gcagcggacg cgctggcggc gtggctgggt ggccagccgc acgtcgcttc
     6661 cgcggccaaa cgcgcggccg gcgtccgggg ggacctcgac cacttcgggg cgatgtcgtt
     6721 cctgcagggc ctgctgacgg tgctgcgcga tgccggacac ccggggctgt tgttggtgtt
     6781 ggacgaggtg gagaccctgc aacgggtgcg tggcgacgtc cgcgacaagg cgctcaacgc
     6841 gctacggcag ctcatcgacg agatcgacgc cggacgttac cccggcctgt acctcgtgat
     6901 caccggcacg cctgcgttct tcgacggccc gtcgggagtg ccccgcttgc cacctctggc
     6961 gcagcgactc cacacggact tcaccaccga cgcccgattc gacaaccctc gtgcggtgca
     7021 ggtgcggctg cccgggttcg acagagccgc cttgcttgcg gttgggcaga gggttcggga
     7081 cttgtacgcc gacggcactg acaacgccgt ccgcgtgcga tcaatggtcg acgacgccta
     7141 cgtggcttca ctggcggacg cggtcaccgg atcgctgggc ggcaaggtcg gcatcgcgcc
     7201 gcgcgtcttc ctcaagaagc tggtcgccga cgtcctcgat cgagtcgacc agttcgcgga
     7261 cttcgatccc cgtcagcact acgcactgac gatcggtgac ggcgacctga ccgaggtgga
     7321 gcgcaacgcc gcgcgcccac cggcgcgcgc ggacggcgtc gcgctcgagc tgtgacgtcc
     7381 ggcgccgcct gatgaccggg ttcgacctgc tgcaccctgt cgtgcagcac cacgtcgtca
     7441 acacacttgg ctggtcagcg ctgcggcctc tgcaggactc cgccgtcgaa ccggtgctcg
     7501 caggctcgga tgcactcctg ctggcaccga ctgcgggagg caagaccgag gccgcgctct
     7561 tccccctctt gagccggatg gccaacgagg attcgcgggg cacctcggtg ctctacctcg
     7621 ccccgctgcg cgccctcctc aacaacctcg aaccccgggt gcgcggctac gcccgctggc
     7681 tcgggcggga cgcgtcggtg cggcacgggg acacgagccg gggcgtccgg cgtaggcagg
     7741 cggtcgagcg tccagatgtg ctgctcacca cccctgaatc gctggagtcc attctcgtgt
     7801 cggcaaccgt ggatccccgc gtcgtcctgg ccgacgtgcg agctgtgatc gtcgacgagg
     7861 tgcatgcgtt cgccggcgac gaccgaggtt ggcacctgct cgccgtcctc gaacgactca
     7921 cccgaatcgc aggtcgccct ctgcagcgga ttggactgtc ggcaacggtg ggcaacgcgc
     7981 gggagttgct tacttggctc cagggcagca gcgccggcgt ccgacccggt gtggtggtgg
     8041 ccccgcacgc cggaggcggg cctgtgcctg agctcgagct ggactacgtc gggtccgtcg
     8101 acaacgcctc cgtcgtgatt gccagcctgc atctcgggga gaagcggctg gtgttcgccg
     8161 actcgcggcg agtggtcgaa cggctcgcgg ggcgactaca cgacgccggt gtcgagacct
     8221 tcgtgtctca ctccagcctg tcagcggtgg aacggcggcg cgctgaggcc gcgttcgccg
     8281 aatcgaggga ctgcgtgatc gtgtcgacct cgacgctcga actcggcatc gatgttggag
     8341 acctcgatcg cgtggtgcag attggggctc cgggctcggt ggcctccctg cttcagcgtc
     8401 tgggtcggac gggacggcgt ccgggaacgg ttcggaacat cctgatgctc gccacggacg
     8461 acaccgaact cctgcgtggg ttcgggctcg ccctgctctg gtctgagggc tacgtcgagc
     8521 ccatccgtcc cacgcccaca ccccagcacg tggcggccca gcagctgctt gcgctggcgc
     8581 tgcaggaggg gcgggttgga cgcctcacct ggccagagtg gctgggcggc ttggcgctgg
     8641 cgtccccgag agcccttggc gtgatcgccg actggctggt ggagacgggg cacctcgatg
     8701 tggacacgga catgttgttc gtgggtccag aggccgagcg caagtacggt cgtcggcact
     8761 tcatggaact catgacggtc ttcacgactg accctcaggt gacggttctc cacggacgcg
     8821 acgagatcgg cgtcgtcgac cccatggtgt tgctgtccaa ggtgaccggt ccccgggtca
     8881 ttgcgctcgg cggtcggtcg tggaaagtga ccggaatgga ctgggaacgg cggcgagcgt
     8941 acgtcgaacc ggcgccagga ccggggctgg cgcgttgggc gggtacggga gcgccgcccc
     9001 gaagcttcgc gctcactggt gcgatgcgtc gcgtgctgtt aggccacgac cctgccggtg
     9061 cgatgatcac gaagcgggct gaggcgcgga ttggcgagtt gagggtgcag tgggatgctc
     9121 gggtcgaccc gacgcggtca gtggtgatgc gtgattccga gggtctccgt tggtggacct
     9181 gggctggcag ccgcggcaac gccgtcctgc acgctgcctt gcaagccgtg gcaccgcaac
     9241 ttctcgaccc gatcgagacg ttcgacaacg aacggatcac cctccaccca gacgcgcgcg
     9301 cccatgacct cagcggtgcg ctgcgcgaag cccgggttcg tttcggcagc gatcttgctg
     9361 aggtccatcc ccgggtggac cgtcgggcgg tggaagggct caagttcgcc gatctgctcc
     9421 cacccgaatt ggcggagggc accctcgcgg cccggatcgg tgacgtggat ggcgcgcgga
     9481 gggtgctgag tctgggtgtc gtcgagcgca ttgtgagcag tatctgaggc gcaggtggtg
     9541 cgtccgcgtc atccgagcag tgtgaccgcc aagacggcgc catggccagc catggtccga
     9601 aggggaaagc cgtgcgcttc cgccggaacg gggtggccag aacggccgcg acgcaggcaa
     9661 tcaggaatgc ccaccacgca gcgcggaggc taaccgctgc ggttgcggcg ccaatgatcg
     9721 ggatgtactt gccgtcgccc caaccgaaac cgccgggccg gacccggtcg aggatcgaga
     9781 agaccacgaa cgcgactacc gcaccgacgg cggctggatg gcaatgctga ggtcgccggt
     9841 gaggatggcg gcggctccca cccccaaagc cacgagggcg ccgtggaccg caagaaggcg
     9901 gttgggcaac cggcggacgt cgaggtcgat ggcggacagc cacgggccaa accacgcgag
     9961 cgacagggtg ggcaggagca acggccagcg gtcgactccg aagcgccacc cgaggagtcc
    10021 actggccgcc gccgttgcga cggggatcca ccagcgcggc cccgggtagg gctggtcgac
    10081 ctctccgtcg cggcggtacg cgaggtccgc caaccgagac ctgatggcca agccgatgaa
    10141 ggcgccgaga aggctcaggg cagcggtgaa catgacgtcg tttccgttca tggctggggt
    10201 ccatgacgga ctggccggga cgttggccag tcgagcgcca tcatcgttgc gtcgcctgac
    10261 atcagcgggt cggacgggtc ttcttgggcg gacgaccaga gggcgcaccg gcggacgctt
    10321 gccgttcttc ggcggagtag ccgatgtccg ccgctggcgc tccggttgcc ctggctgcgc
    10381 ggaggcgtcc gaccacggcc gaggtggcgg ccatcgcagg gccggaggcg tcccgggagt
    10441 aggtcatcga ccggttggcc tgcacgccga ggctggcgcc gacctcgata gcgtcctggt
    10501 cagcgcccat gtagaggaac tcccagttgt aggtcttcgt ctgctgctgc acgagggcct
    10561 tgatggcggg atgggtccat tcgcgggagg cgttctcgta cccgtcggtc atgatgccga
    10621 cgatgacggt gccgggccgc tggcactctg gcagtgaggc gaggtgggcg ccggcgtccg
    10681 tcacgaggcg gccgatggcg tccaacaggg cggtggtgcc gcggggttgc aggtcgaggc
    10741 cggggacctc ggcgatgggc cggtcggtgt agaccacctc gtagcggtcg tcgaactggg
    10801 ccagagtgac gcgacagtca ccggcatgct ggcgctgctc ggccatgaag gcgtcgaagc
    10861 cgccgatcgt gtcgtcgcgg atggactgca tcgacccgga acggtcgagc aagaagtaca
    10921 ggtgggtgcg gttggggtcg gtcatcgtcg tctccttcga gagtgtcagg tgtcgcggcg
    10981 acgccggaac ctgagcggtt cgatcgggtg ttcgggggtg ggggcggggc gcccgatttc
    11041 ggcgtcgtaa cgggcgcgct cgaccgaggt cctggtcacg cgcccttccg gggtggcgta
    11101 gggcatcagc cgggcggtgc gcgccagccg cggtccgacg gcgttctccg aaaggctggc
    11161 caactggccg gcctggcgca gcgacgtccc ttcctcgagg acggcggcgg ccatcgcctc
    11221 gtcgatcgcg cgggtcaact gctcctgcgc ctcgctcaga aggggtaggg cgtccttcgc
    11281 tgtcgtggcg cccgcggcgt gcgtgagcgc ggcggccgcg tcgctgatca gagcgtccag
    11341 ttcgtcccgt ccggttccca cgaaaccaag catacgcagt aactgcggat acgcaagaag
    11401 cgtcgggtcg cttctgacgc ggccgagccg ctcacgtata tggcaagaat cccggttgtg
    11461 ctcgaaacgt tcagttggcc gacgcgctcg gcagctgagg cagcgttccg cgggatccta
    11521 cggaactccg gctactcggt cggtgacgcc gtctcggacc cggtccacga cctcgcatcg
    11581 cacgctgatg accgtccctg ggtccctttc gataggggga ccgatcccgt gctggaatgt
    11641 gcgcagaggc tgtccgagca gggactcccg ccgtcgccca gcgtggcgca ggacaggagg
    11701 accgctcggt gagtcatccc tgtcgtcggg ctgtttgagt cgaagccatc gctcgcgcac
    11761 gggacccgag tcgaggacag cctgcggtgg gaccgtctgc ggtcatgcac ccctactcca
    11821 ataccgcctc tagcaccgac cgccgccgcc gcaattggag caggatgctc gtggacgggc
    11881 aactccgctg ggctggttcg ccgagtctgt ggagcccacg gtgccccccg ggcactcggc
    11941 cgcgaatccc tgcagtcagg cctcggtggc ttcaatctgc tcgccggcaa cggaagtgag
    12001 ttcgagaggg cgaaccgtcg gaacttccga ttcgggtagg ttcatgtaac ttgccagtag
    12061 ttcgccgagg cgcccggcat ctcgacgcgc gccgtcgttc ttcgcccagg cggggaagtg
    12121 cagcgtcagg ctggtgttct ttgtgatgac atccacaacc ggaggcttgt tcacaggatt
    12181 gtggcggcgc acgtagcgga tgtcgccggt gccgatagag atgtcaatcc cgcccacttg
    12241 gcgcaaggag tcctgcttcg tgatgaggag tcgccggtcg gtgattgcca cccagccacc
    12301 accaaggaac ttccagcggt cgcatgatgc gtcggccaga gcgagcaccc gctcatccag
    12361 ctcgagacgg agcgggagga tgcggacgag ttcccccgga accttgtctt cgaagacgag
    12421 gatcgacggc aacgccagcg gttggggttc cttcggggcg tcctgcccgc gcactgatgc
    12481 caacgcttcc tgcagttgcc gcaccatttt ggccgcgtcc ttcgctgctc gagcggtggc
    12541 gccgatcttg aacgtcttct tgccaggtaa ctcggcgatg atggcgaact cacgcgcgag
    12601 ctgatcgagg agccagttcg tctcgtcgag ggtcttctcg tggaggtctt gcacctccgc
    12661 gatgaggttc ttcaacagcc tctcatcctg cggattggag cctgcactcc tggacagatg
    12721 cgccgcgcgc atcgcctggt gggtgaacca agaggtctga gccaagagca gagcctgagc
    12781 atccgcgacg atcgcccgtc cgttgtcaag gaggtacttc tggcgcgtct ggtgcccacg
    12841 tttcgtgcgg agtgcgctca catgctgctg gacggccgat ttgaaaacgt cccactggct
    12901 cgtcagcttt ccctcgtagc cacggacctc ttgatagatg gcatcggtga cctccccgat
    12961 ctgacgggcg tgcccgaact ccctcatcag ggtcttgtag tggccggtaa cagaggacca
    13021 ttggtcgcgc cgcaccacct ccagcaccgt cgaagtcagt tcgaggttgt gctcggcgag
    13081 tcgagcgatc tggtttagct gtgcctggat caccatcaag ctgatggccg gccccatggc
    13141 ggcgacgact gaggctgtgg tcgccgcagt cgccgggagc caccgcactt gggcggcgaa
    13201 cttgctcccg ctcgcgagcg tgccgagatt ccatcc
//
LOCUS       DAOSUG010000013        13138 bp    DNA     linear   ENV 10-JUN-2024
DEFINITION  MAG TPA_asm: Micropruina sp. isolate AMR_MDS_2195
            NODE_2808_length_13138_cov_4.356875, whole genome shotgun sequence.
ACCESSION   DAOSUG010000013 DAOSUG010000000
VERSION     DAOSUG010000013.1
DBLINK      BioProject: PRJNA925477
            BioSample: SAMN32864262
            Sequence Read Archive: SRR4236673
KEYWORDS    WGS; ENV; Metagenome Assembled Genome; MAG; Third Party Data; TPA;
            TPA:assembly.
SOURCE      Micropruina sp. (wastewater metagenome)
  ORGANISM  Micropruina sp.
            Bacteria; Bacillati; Actinomycetota; Actinomycetes;
            Propionibacteriales; Nocardioidaceae; Micropruina.
REFERENCE   1  (bases 1 to 13138)
  AUTHORS   Abdulkadir,N., Saraiva,J.P., Zhang,J., Stolte,S., Gillor,O.,
            Harms,H. and Rocha,U.
  TITLE     Genome-centric analyses of 165 metagenomes show that mobile genetic
            elements are crucial for the transmission of antimicrobial
            resistance genes to pathogens in activated sludge and wastewater
  JOURNAL   Microbiol Spectr 12 (3), e0291823 (2024)
   PUBMED   38289113
REFERENCE   2  (bases 1 to 13138)
  AUTHORS   Abdulkadir,N.
  TITLE     Direct Submission
  JOURNAL   Submitted (24-MAR-2023) Environmental Microbiology, Helmholtz
            Center for Environmental Research -UFZ, Permoserstr 15, Leipzig
            04318, Germany
COMMENT     The annotation was added by the NCBI Prokaryotic Genome Annotation
            Pipeline (PGAP). Information about PGAP can be found here:
            https://www.ncbi.nlm.nih.gov/genome/annotation_prok/
            
            ##Genome-Assembly-Data-START##
            Assembly Method        :: SPAdes v. 3.11.1
            Genome Representation  :: Full
            Expected Final Version :: Yes
            Genome Coverage        :: Not Applicable
            Sequencing Technology  :: Illumina MiSeq
            ##Genome-Assembly-Data-END##
            
            ##Genome-Annotation-Data-START##
            Annotation Provider               :: NCBI
            Annotation Date                   :: 05/04/2023 04:57:10
            Annotation Pipeline               :: NCBI Prokaryotic Genome
                                                 Annotation Pipeline (PGAP)
            Annotation Method                 :: Best-placed reference protein
                                                 set; GeneMarkS-2+
            Annotation Software revision      :: 6.5
            Features Annotated                :: Gene; CDS; rRNA; tRNA; ncRNA
            Genes (total)                     :: 2,776
            CDSs (total)                      :: 2,750
            Genes (coding)                    :: 2,714
            CDSs (with protein)               :: 2,714
            Genes (RNA)                       :: 26
            tRNAs                             :: 24
            ncRNAs                            :: 2
            Pseudo Genes (total)              :: 36
            CDSs (without protein)            :: 36
            Pseudo Genes (ambiguous residues) :: 0 of 36
            Pseudo Genes (frameshifted)       :: 2 of 36
            Pseudo Genes (incomplete)         :: 32 of 36
            Pseudo Genes (internal stop)      :: 2 of 36
            ##Genome-Annotation-Data-END##
FEATURES             Location/Qualifiers
     source          1..13138
                     /organism="Micropruina sp."
                     /mol_type="genomic DNA"
                     /submitter_seqid="NODE_2808_length_13138_cov_4.356875"
                     /isolate="AMR_MDS_2195"
                     /isolation_source="Activated sludge of municipal
                     wastewater treatment plants"
                     /db_xref="taxon:2737536"
                     /environmental_sample
                     /geo_loc_name="Canada"
                     /lat_lon="49.25 N 123.1 W"
                     /collection_date="2015-06-16"
                     /metagenome_source="wastewater metagenome"
                     /note="metagenomic"
     gene            94..240
                     /locus_tag="PLF56_00870"
     CDS             94..240
                     /locus_tag="PLF56_00870"
                     /inference="COORDINATES: ab initio
                     prediction:GeneMarkS-2+"
                     /note="Derived by automated computational analysis using
                     gene prediction method: GeneMarkS-2+."
                     /codon_start=1
                     /transl_table=11
                     /product="hypothetical protein"
                     /protein_id="HOQ52144.1"
                     /translation="MRRPFGDRTLWMLAVVAALALIAAIVFGVAINTQAPLQPLPVPS
                     RASA"
     gene            complement(473..985)
                     /locus_tag="PLF56_00875"
     CDS             complement(473..985)
                     /locus_tag="PLF56_00875"
                     /EC_number="5.2.1.8"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_002525071.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_function: GO:0003755 - peptidyl-prolyl cis-trans
                     isomerase activity [Evidence IEA];
                     GO_process: GO:0000413 - protein peptidyl-prolyl
                     isomerization [Evidence IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="peptidylprolyl isomerase"
                     /protein_id="HOQ52145.1"
                     /translation="MAQTATLHTNHGDIVIDLFPDQAPKTVDNFVGLATGTKEYLDAA
                     SGARTTGNFYDGLTFHRIIDGFMIQGGCPQGSGRGGPGYTFGDEFHPELTFNRPYLLA
                     MANAGPGTNGSQFFITVTPTPHLNRKHTIFGEVTDEASQQVVDEIAQVRTGYGDRPVE
                     PVVIESVTIA"
     gene            complement(1081..1731)
                     /locus_tag="PLF56_00880"
     CDS             complement(1081..1731)
                     /locus_tag="PLF56_00880"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_017934331.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="LysE/ArgO family amino acid transporter"
                     /protein_id="HOQ52146.1"
                     /translation="MLLSALAGLGLGFSLIVAIGAQNLFVLRQGLRREHVLVTAAICA
                     ASDAVLIVLGVSGVGLVLQAVPWLLTAVRWAGAVFLVCYGLLAARRAWRPSGDTLLMD
                     AAMPAPAPSSGSVALQTRARLAPVVMTCLALTWLNPHVYLDTVFLLGSVANSHGEARW
                     AFAAGAVLASLVWFFGLAIGARLLSPWLSTPRAWRLLDGLIAVVMIALGVGLVLVH"
     gene            1803..2696
                     /locus_tag="PLF56_00885"
     CDS             1803..2696
                     /locus_tag="PLF56_00885"
                     /inference="COORDINATES: protein motif:HMM:NF002964.0"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_component: GO:0005829 - cytosol [Evidence IEA];
                     GO_function: GO:0003677 - DNA binding [Evidence IEA];
                     GO_function: GO:0003700 - DNA-binding transcription factor
                     activity [Evidence IEA];
                     GO_process: GO:0006355 - regulation of DNA-templated
                     transcription [Evidence IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="LysR family transcriptional regulator ArgP"
                     /protein_id="HOQ52147.1"
                     /translation="MRIPLDLAQTLAAIIDEGTLDAAARRLHLTPSAVSQRLKALERQ
                     VGRVLLVRTKPARATDAGRAVVRLARQLALLEHDALSELGDVEAGRVSVPLAVNADSL
                     GTWFLAPLARLSARHPVVFDLHRDDQDFTVGLLETGTVMAAVTSRSEPVAGCRVTALG
                     VMRYQAVATPTFVRDWLPDGPSPESLARAPVVDFDRRDDLQTEWLVQRGADAFTAPRH
                     FVPASNDYARAVIAGLGWGMLPGFQSERQLSDGELVALGGEPIAVPLFWQQWNLRSPL
                     LDEVALEIVAEARRVLDQVDG"
     gene            2811..4247
                     /locus_tag="PLF56_00890"
     CDS             2811..4247
                     /locus_tag="PLF56_00890"
                     /inference="COORDINATES: ab initio
                     prediction:GeneMarkS-2+"
                     /note="Derived by automated computational analysis using
                     gene prediction method: GeneMarkS-2+."
                     /codon_start=1
                     /transl_table=11
                     /product="hypothetical protein"
                     /protein_id="HOQ52148.1"
                     /translation="MKRKKPLSAVESTAKSAADSGQAALDEAVQRLTPLVEQAGDYVD
                     DARKRSVEFASDAYDRVQPALQHGAELASDAYERLEPALKDVYDRIEPTFRTAGALAS
                     EAYERVQPTLKKAGKQSAKFAAETLDRVHPALDDALDKVTPAVDSAVKKVRPVVDDTL
                     EKVAPTVDAARDRVQNDLLPWLAAALYKAADLAKDEVHEVLPATRKRRSVGKTLGLIA
                     LAGAVLAGVVYAVRRLLAPQDSGWETHEPSRPYVADPVSDVVNKVKDSSGGVVDDIKA
                     AAAKAGDAVSHTVHDLKDKAAGLVDDAKGAAGDALHRASGTADDLKDRAGDVVDDAKD
                     AASDLKDKAGDVVDDVKDAAGDAADDAADRADEVKDAVGDAVDDVKDAAGDAAENVEE
                     ATDKAKQSAEKASKDAKGDASPLAESPYGEGSYVGAEPPQGFVIKGNERSMKYHLQGS
                     GGYDRTITDVWFDSTEAAERAGFVRAQR"
     gene            4467..4973
                     /locus_tag="PLF56_00895"
     CDS             4467..4973
                     /locus_tag="PLF56_00895"
                     /inference="COORDINATES: ab initio
                     prediction:GeneMarkS-2+"
                     /note="Derived by automated computational analysis using
                     gene prediction method: GeneMarkS-2+."
                     /codon_start=1
                     /transl_table=11
                     /product="hypothetical protein"
                     /protein_id="HOQ52149.1"
                     /translation="MREEHDDDESYRLQRPLEPGPDEPWVEAAPFRAHVLHTLEVADI
                     PWPVFAIETGIPVPAIRSLLFGRQGRPLKRLTPRLAGKLLRVQPPDLAGLHRTQVRAD
                     VTAKRLGGLLRAGVDPLRLARWCDLSPHELARLVDGEAPSCSRVTESLVLAAERMLFA
                     PVLGQRAA"
     gene            4984..5637
                     /locus_tag="PLF56_00900"
     CDS             4984..5637
                     /locus_tag="PLF56_00900"
                     /EC_number="3.4.23.43"
                     /inference="COORDINATES: protein motif:HMM:NF013632.2"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_component: GO:0016020 - membrane [Evidence IEA];
                     GO_function: GO:0004190 - aspartic-type endopeptidase
                     activity [Evidence IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="prepilin peptidase"
                     /protein_id="HOQ52150.1"
                     /translation="MTWQWLAPLVVAAAVASVTPRVLLALPAPIDEPDSRPYDALATR
                     AVALTTFLLVGVAGVVVAALAPAATSAWLGLGTAGVLAAVVDARTGYLPTVLMRVGWV
                     LTAGGLIAATASTNDWQLLARAAGGALAATALFYTFHRIGGGFGFGDVRLAPIVAAPA
                     AALGWTTLAGALLLGGLLGVAWGLTWQALGRGKAFPYGPALVAGPYLALLAGATLPP"
     gene            complement(5669..6718)
                     /gene="trpD"
                     /locus_tag="PLF56_00905"
     CDS             complement(5669..6718)
                     /gene="trpD"
                     /locus_tag="PLF56_00905"
                     /EC_number="2.4.2.18"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_014847074.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_function: GO:0004048 - anthranilate
                     phosphoribosyltransferase activity [Evidence IEA];
                     GO_process: GO:0000162 - tryptophan biosynthetic process
                     [Evidence IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="anthranilate phosphoribosyltransferase"
                     /protein_id="HOQ52151.1"
                     /translation="MGYTWPDVLTGLISRENLSAEAAGWAMDEILAGNATPVQMAGFL
                     VALRAKGETVDEIAGLAEAMLARATPIGLPSEAVDVVGSGGDRANTVNISTMAAVVAA
                     GAGAKVVKHGNRAASSACGAADVLETLGVVLDLRPERQAEVLHEAGIVFLFAPLYHPS
                     LRHAGTARRELGIQTPFNFLGPLANPARPAAQAVGVANARMAAIVAGVLAARGTRGLV
                     FHGDDGLDELTTTTTSDVWLIAGGDVVATRLNPADLGLADAARDDLVGADAAHNAGVV
                     RGVLGGERGPVRDIVLLNAAAALLADTGPDLGRDVTDQVAEQLQRAERSIDSGAAATT
                     LQTWVAATRRAAEGR"
     gene            complement(6721..7125)
                     /locus_tag="PLF56_00910"
     CDS             complement(6721..7125)
                     /locus_tag="PLF56_00910"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_014847075.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="hypothetical protein"
                     /protein_id="HOQ52152.1"
                     /translation="MTQQSAKILLYSDDRTTREQVRLALGQRVAADLPDLEIFEVATQ
                     PAVLAAVDAGGLDLLILDGEATPSGGMGLAKQLKDEVAQCPPILVLIIRVADAWLATW
                     SRADAAVMYPVDPVRLPAAVAELLRARLAQAV"
     gene            complement(7380..8216)
                     /locus_tag="PLF56_00915"
     CDS             complement(7380..8216)
                     /locus_tag="PLF56_00915"
                     /inference="COORDINATES: protein motif:HMM:NF017206.2"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="NAD(P)H-binding protein"
                     /protein_id="HOQ52153.1"
                     /translation="MSVLVVGASGAVGARVVAGLVAQGVPVRATSRDPRKLALPPQVG
                     VFAADLNVPASFGPALQGVERVFLYADLGDPEALVAELVRSGVRQAVLLSSSSVTFPG
                     AEEDFNGARFVRVERAIDGSGLDYTFLRPGSFASNAARWSWSVKTAGVVPLPYPEAVQ
                     VPVHEADIADVAVVALTGETLLGARPVLTGPERLSLREQVATIGSVIGRPVSVREQSE
                     RESSALLSQFVTTEWSSQIIKGWRESVGARPEISGEYTRITGQSARTFRAWVTDHVEL
                     FR"
     gene            8312..9010
                     /locus_tag="PLF56_00920"
     CDS             8312..9010
                     /locus_tag="PLF56_00920"
                     /inference="COORDINATES: protein motif:HMM:NF014906.2"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_function: GO:0003677 - DNA binding [Evidence IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="helix-turn-helix domain-containing protein"
                     /protein_id="HOQ52154.1"
                     /translation="MTSPAEPISRRDRPAKAPLSRRSILDAAIELIRVRGVAAVTLKD
                     VAEAVQTGPASLYAYFRNRDELLECVLDAAYAQVELVHPLDGDWRGALAATIVNTIEK
                     LESFPGLATVALGAIPTREGALRLAEHELTLMELGGIDADAAALGVDLIAQFAAATAI
                     ERAVPGKRERGHTEREQLKAAYGGADPQVYPRVARLASALTAPDEPSRRDFAIQAILN
                     GLTSTPYPPTRPTP"
     gene            9547..10218
                     /locus_tag="PLF56_00925"
     CDS             9547..10218
                     /locus_tag="PLF56_00925"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_013865036.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="heme-copper oxidase subunit III"
                     /protein_id="HOQ52155.1"
                     /translation="MPSGAVHPIAPARLLPPKGRPDLVAVGTWIWLASDLMFFAALFA
                     AYFNLRSITNAAASAAGTESMWQWGTAHFNLPFAATNTLILILSSVTCQMGVWKAERG
                     QVGRTGSLLQVGQWGLREWYILTFIMGAIFVAGQATEYATLISEGFTLSTNAYTSAFF
                     LATGFHGLHVTGGLIAFLFMLGRTYVSRTFTHEQAVHAVVTSYYWHFVDAIWIILFSV
                     IYILR"
     gene            10243..11043
                     /locus_tag="PLF56_00930"
     CDS             10243..11043
                     /locus_tag="PLF56_00930"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_002532211.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_function: GO:0009055 - electron transfer activity
                     [Evidence IEA];
                     GO_function: GO:0020037 - heme binding [Evidence IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="c-type cytochrome"
                     /protein_id="HOQ52156.1"
                     /translation="MRFLTAKRRHRAARPALLVLALLLMGALYAAVAPAARVSADTGN
                     SQQVAEGKALFQVTCSSCHGMNGEGTSQGPSLVGVGAASANFQVGTGRMPMANPGAQA
                     PQKRTNYTADEIAALSAFVASLGPGPAIPTEEQYSGEGLSAEELAKGGELFRTNCSAC
                     HNFEGAGGALPNGKYAPSLQGVSGQHIWEAMRTGPQQMPVFSAGALPDDNVRAIVGYL
                     ETLHAQPSSGLTFGALGPVAEGLFAWIIGIGGLCLIAVWIAARGARAK"
     gene            11040..12104
                     /locus_tag="PLF56_00935"
     CDS             11040..12104
                     /locus_tag="PLF56_00935"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_015069957.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_function: GO:0051537 - 2 iron, 2 sulfur cluster binding
                     [Evidence IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="Rieske 2Fe-2S domain-containing protein"
                     /protein_id="HOQ52157.1"
                     /translation="MSIETRQSETEVERFPVPDPGLEPHIERWTDADKSAGDRAYRQV
                     LMMLGAVPVLAIAFVVIYFTVPRTSFVDIFGMQWDALTFLLGLTGGLATLLIGLACIH
                     WAKTMMGDEEIQEARHSAASSAEDREVFAAEFAKGAEQSGLGRRKLIGGALGGALGLI
                     AVPAVVLFADMGPWPTAAVRKETIERTIWAQGVKLVNDITRRALKASDLEIGQLVNAQ
                     PENLWDHHGTDFQVEKAKASIIIVRMDPTQIKIPESRKDWQVGGILCYSKICTHVGCP
                     ISLWEQQTHHLLCPCHQSTFDLSNSGVVVFGPAARSLPQLPITVDADGYLVARSDFTV
                     PVGPSFFERDSNHDFVEGDR"
     gene            12104..>13138
                     /locus_tag="PLF56_00940"
     CDS             12104..>13138
                     /locus_tag="PLF56_00940"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_002513974.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="cytochrome b"
                     /protein_id="HOQ52158.1"
                     /translation="MAKPANVSTESPVLDTERPPKPDGLPSAVTGAATWADERLGLAS
                     LLKKNLRKVFPDHWSFLLGEIALYSFLILLLTGVFLTIWFKPSMAEIEYQGSYQLMRG
                     LPMSEAFASTLNLSFDVRGGLLVRQIHHWAAMLFIAAMTVHMLRIFFTGAFRKPREIN
                     WLIGVGLFSMSLIEGFAGYSLPDDLLSGTGLRFVDGLIRSIPLVGTWLEFFIFNGEFP
                     GDLVIPRLYMVHILLVPGLMLGLIAAHLALVVYHKHTHYPGPGRKDGNVVGYPLFPVY
                     MAKAGGFFFVVFGVSILMGATMQINPVWTVGPYNPAQVSAGSQPDWYMGWVEGAVRIM
                     PNWEWHLGSTT"
BASE COUNT         2132 a         4637 c         4329 g         2040 t
ORIGIN      
        1 gaacaccgag acgcacatcg catttcgcgc ctacagttcg cacgttctcg aggcggcgtt
       61 cagtctgggt gctgagtgag gcacggctct tccatgcgtc gccccttcgg cgatcgcacc
      121 ctgtggatgc tcgcggtggt ggccgcgctg gcgttgatcg ccgccatcgt cttcggcgtc
      181 gcgatcaaca cccaggcacc cctgcagccc ctacccgtgc cctcccgcgc cagcgcctga
      241 ggttcagccc ggatcctgac gatcaacacg gatcgacatt catcgctcca cgctcagcgc
      301 tgagccacgc tcagcccgga tcccggctca gcgcgcgccg accagggcgg ggccgggttg
      361 cgtgggccga aacctcgtgc aggtgagggc ctcgatccgc tcggagcacc cgtgcattga
      421 tgggtcgtcg agcgcggcag gtcgcgcgtc cgtgctgacc aggcaaccag gctcaggcga
      481 tggtgaccga ctcgatcacc acgggctcga ccggacgatc gccgtacccg gtgcgcacct
      541 gggcgatctc gtcgaccacc tgctggctgg cctcgtcggt cacctcaccg aagatcgtgt
      601 gcttgcggtt caggtgcggc gtcggcgtca ccgtgatgaa gaactgcgag ccgttggtgc
      661 cggggccggc gttggccatg gccagcagat agggccgatt gaaggtcagt tcggggtgaa
      721 actcatcgcc gaaggtgtag ccggggccgc cgcgtccgct gccctgcggg cacccgccct
      781 gaatcatgaa gccgtcgatg atgcggtgaa aggtcaggcc gtcgtagaag ttgccggtgg
      841 tgcgagcgcc cgacgcggca tcgaggtact ccttggtgcc ggtggccaga ccgacgaagt
      901 tgtccacggt cttgggggcc tggtcaggga acaggtcgat gacgatgtct ccgtgattgg
      961 tgtgcagggt cgcggtctgc gccacggcgg tgtcctttca gttcggcggc ttcggcttat
     1021 cgctgcctgc cgccgcaggg cttgggtccg aaccatcctg acacacccgg gccaacccgg
     1081 tcagtgcaca agcaccagtc ccacgcccag agcgatcatc accacggcga tcagcccgtc
     1141 cagcaaccgc caggcccgag gggtcgacag ccacggcgac agcagccgcg ctccgatcgc
     1201 caggccgaag aaccagacca ggctggccag caccgccccg gcggcgaacg cccaacgcgc
     1261 ctcgccgtga gagttcgcca ccgaaccgag cagaaagacc gtgtcgaggt agacatgggg
     1321 gttgagccag gtcaacgcga ggcaggtcat caccacgggc gccagtcgcg ccctggtctg
     1381 aagtgcaacc gagccggacg acggcgcggg cgcgggcatc gcagcgtcca tcagcagcgt
     1441 gtcaccgctg ggccgccacg ctcggcgcgc tgcgagtagg ccgtagcaga ccaggaacac
     1501 cgcgccggcc caccgaaccg cggtcagcag ccatggcacc gcctgcagca ccagcccgac
     1561 gcccgagacg ccgagcacga tcagcaccgc atccgaggcg gcacagatcg cggcggtcac
     1621 cagcacgtgt tcgcgccgca gcccctggcg cagcacgaac aggttctgcg cgccgatggc
     1681 aacaatgagc gagaagccga gcccgaggcc ggcgagagca gaaagcagca ccccttgacc
     1741 gtaagccgcc tcctggcgtc agtccagcta cagattctgt tgatgcatta gcatctttca
     1801 cgatgcgcat ccctctcgac ctggcccaga ccctcgccgc aatcatcgac gaaggcacct
     1861 tggatgccgc cgcccgccgc ctgcacctca cgcccagcgc ggtcagtcaa cgacttaagg
     1921 cgctcgagcg gcaggtcggc cgggtgctgc tggtgcgtac caagccggcc cgggccaccg
     1981 acgccggccg agccgtcgtg cgactggcca ggcaactggc actgctcgaa cacgacgcgc
     2041 tgagcgagtt gggcgacgtc gaggccggac gggtgagcgt gccgctggcc gtcaacgccg
     2101 actcgctcgg cacctggttt cttgcgccgc tggcccgact gagtgcgcgc catccggtgg
     2161 tcttcgacct gcaccgcgac gatcaggact tcaccgtggg cctgctcgaa accggcaccg
     2221 tgatggcggc ggtcacctcg cggtccgaac cggtggccgg ttgccgggtg accgcgttgg
     2281 gcgtgatgcg ctaccaggcc gtcgccacgc cgaccttcgt ccgcgactgg ctgccggacg
     2341 gcccctcccc cgagtcgctg gcccgggcgc cggtggtcga cttcgatcgt cgcgacgacc
     2401 tgcagaccga gtggctggtg cagcggggcg ctgatgcctt caccgcacca cggcacttcg
     2461 tgccggcctc caacgactat gcgcgggcag tgatcgcggg tctgggctgg ggaatgctgc
     2521 ccgggttcca gtccgagcga caactaagcg acggcgaact ggtggcgttg ggtggcgaac
     2581 cgatcgccgt tccgctgttc tggcagcaat ggaacctgcg ctcccccctg ctggacgagg
     2641 tggcgctcga gatcgtcgca gaagcacgtc gggtgctcga ccaggtggac ggctgaacag
     2701 cacacccaca acggtgggaa tgccgcgcag acctgttcgg gcacccgagc cgacgggtac
     2761 gttgggtgcc acgcggtaca gccgcgttca tcccaaccac tggaggaaca gtgaagcgaa
     2821 agaagccgct gtcggccgtc gaatcgaccg ccaagtcagc cgcagattcc ggccaggccg
     2881 ctctcgacga ggcggtgcaa cgtctcaccc ccttggtcga acaggccggc gactacgtcg
     2941 atgatgcgcg caagcgaagc gtcgagttcg cctccgatgc ctatgaccgt gtgcagcccg
     3001 cgctgcagca tggtgccgag ttggcatccg atgcgtacga acggctcgag ccggccctca
     3061 aagacgtgta cgaccgcatc gagcccacgt tcaggaccgc cggggctctg gccagcgagg
     3121 cctacgagcg cgtccaaccg acgctgaaga aggcgggcaa gcagtcggcc aagttcgccg
     3181 ccgaaaccct cgaccgggtg cacccggcgc tcgatgacgc gctcgacaag gtgacgccgg
     3241 ccgtcgacag tgcagtcaag aaggttcgtc cagtggttga cgacacgctc gagaaggtgg
     3301 cccccaccgt ggacgcggcc cgcgaccggg tgcagaacga cctgctgccc tggctggccg
     3361 ccgccctcta caaggcggcc gacctggcca aggacgaggt gcacgaggta ctgcccgcca
     3421 ctcgcaagcg acgcagtgtc ggcaagaccc tgggactgat cgccctggcc ggggccgtgt
     3481 tggccggtgt ggtctatgcc gtgcgcaggt tgctcgcccc gcaggattcg ggctgggaga
     3541 cgcacgagcc gtcccggccc tatgtggccg acccggtcag cgacgtggtg aacaaggtca
     3601 aggactcctc cggcggggtg gtggacgaca tcaaggcggc cgcagccaag gccggcgatg
     3661 ccgtctcgca caccgtccat gacctcaagg acaaggcggc cggcctggtc gacgacgcca
     3721 agggggccgc gggcgatgcc ttgcaccggg cgagcggcac ggccgacgac ctgaaagaca
     3781 gggccggcga cgtcgtcgac gatgccaagg acgccgcaag cgacctgaaa gacaaggccg
     3841 gcgacgtggt cgacgacgtc aaggacgccg cgggcgacgc ggccgatgac gcagccgatc
     3901 gggccgatga ggtcaaggac gctgtgggcg atgcggtcga cgacgtcaag gacgccgccg
     3961 gtgacgccgc cgagaacgtc gaagaggcca ccgacaaggc aaagcagtcg gccgagaagg
     4021 cgtcgaagga tgcaaagggt gatgcctccc ctttagctga aagcccctac ggcgagggat
     4081 cgtacgtggg ggccgaaccg ccgcaggggt tcgtgatcaa gggcaatgag cgttcgatga
     4141 agtaccacct gcaaggctcc ggtgggtacg accgcacgat caccgatgtc tggttcgact
     4201 cgaccgaggc agctgagcgc gccggcttcg tccgggcgca acgctgatgg cggctggtcg
     4261 cgccactgtc accggtggtt cgaccagctg aaccggaacc tcgaacgccc ggcgcccctc
     4321 gggggcgctg agcacctgcg aacgggggtg gctgcgacgg cggccgcccc cgtttcgcgc
     4381 gctcagacga ccggctctcg ctaagaatct gaaaaaagtt cgcctcgcca ctcacaaaac
     4441 acccctcgaa aagtgcattc aagggtgtga gagaagaaca cgacgacgac gagtcgtacc
     4501 gactgcaacg gccactcgag cccgggccgg acgagccctg ggtcgaagcg gcgcccttcc
     4561 gggcccatgt gctgcacacc ctcgaggtgg ccgacattcc ctggccggtc ttcgccatcg
     4621 agaccggcat ccccgttccg gcgatccgca gcctgctgtt cggccggcag ggccgtccgc
     4681 tcaagcgact cacgccccgc ctggccggca aactgctgcg cgttcagccg cccgacctgg
     4741 cggggctgca ccgtacccag gtacgcgccg atgtcacggc gaaacgtctg ggcgggctgc
     4801 tgcgggcggg ggtcgacccg ctgcgcctgg ccaggtggtg cgatctcagc ccgcacgaac
     4861 tggcccggct ggtcgacggc gaggcgccca gctgttcgag ggtcaccgaa tctctcgtgc
     4921 tggctgccga gcgcatgctc ttcgctccgg tcctcggcca gcgcgcggcc taggattccg
     4981 cgcatgacct ggcagtggct ggcgccgttg gtggtcgcgg ctgcggtggc gtccgtgacc
     5041 ccccgtgtgc tgctcgcgct acccgccccc attgatgagc cggacagccg tccctatgac
     5101 gcgctggcaa cacgtgcggt tgccctgacg accttcctgc tggtcggcgt cgccggcgtg
     5161 gtggttgcgg cgctggcgcc ggcggcgacg tcggcctggc tgggtctcgg caccgccggt
     5221 gtactggcgg cggtggtcga tgcgcgcacg ggctatctgc cgactgtgct gatgcgggtc
     5281 ggctgggtgc tgaccgccgg cggcttgatc gccgccacag cgtcgaccaa cgactggcag
     5341 ctgctcgctc gggctgccgg gggtgcgctg gccgccaccg cgctgttcta cacgttccac
     5401 cgcatcggcg gcggtttcgg cttcggtgac gtgcggctgg ccccgatcgt cgccgcaccg
     5461 gccgctgccc tcggttggac gacgctcgcg ggcgccctgc tgcttggcgg actgctgggc
     5521 gtggcgtggg gactgacttg gcaggcgctg ggccggggca aggcgtttcc ctacggtccg
     5581 gcgctggtgg ccgggccgta tctggcgttg ctcgccggcg ccacgctgcc gccctgagcc
     5641 cggattcggg cgggggcccg acgtgtcgct atcgaccctc ggcggcgcgc cgggtcgcgg
     5701 ccacccacgt ctgcagtgtc gttgcggcag cacccgaatc gatggaccgc tcggcccgct
     5761 gaagctgctc ggccacctga tcggtgacgt cccgaccgag atcggggccc gtatcggcca
     5821 gcaacgccgc cgccgcgttc agcagcacga tgtcgcgcac cggcccccgc tcgccgccga
     5881 gcacgccgcg cacgacgccg gcattgtgcg ccgcatcggc gccgaccagg tcgtcgcggg
     5941 ccgcgtccgc caacccgagg tcggccgggt tcagccgggt ggccaccacg tcgcccccgg
     6001 cgatcagcca cacgtccgag gtggtggtgg tggtcagttc gtcgagcccg tcgtcgccat
     6061 gaaacaccag gccgcgggtg ccgcgtgcgg ccagcacgcc ggccacgatc gcagccattc
     6121 gggcattggc gacgccgacg gcctgcgcgg ccggccgcgc cgggttcgcc agcgggccaa
     6181 gaaagttgaa gggggtctga atgcccagtt cgcgacgggc cgttccggca tgccgcagcg
     6241 acgggtggta caggggcgcg aacagaaaga cgatgccggc ctcgtgcagc acctcggcct
     6301 gccgctcggg gcgcagatcg agcaccaccc ccaatgtctc gagcacgtcc gcggcgccgc
     6361 acgcagagga cgcggcccgg ttaccgtgct tgaccacctt ggccccggca cccgcggcca
     6421 cgacggcggc catggtcgaa atgttgaccg tgttcgcacg gtcaccgccc gagcccacca
     6481 cgtccaccgc ctcgctgggc aggccgatcg gcgtggcgcg ggccagcatg gcctcggcca
     6541 agcccgcgat ctcgtcgacc gtctcgccct tcgcacggag agcgaccaga aagccggcca
     6601 tctgcacggg agtggcgttg ccggccagga tctcatccat ggcccagccg gcggcctcgg
     6661 cggacaggtt ctcgcgcgag atcagcccgg tgagcacatc gggccaggtg taccccacgc
     6721 tcaaaccgcc tgggcgagcc gggcacgcag caactctgcc accgccgccg gcagtcgcac
     6781 cgggtcgacc gggtacatca ccgcggcatc ggcgcgcgac caggtggcca gccaagcgtc
     6841 cgctacccga atgatcagca ccaggatcgg cgggcactgc gccacctcgt ccttgagctg
     6901 cttggccagt cccatgccgc cggacggggt cgcctcaccg tcgaggatca gcagatcaag
     6961 accaccggca tcgacggccg ccagcaccgc cggttgcgtg gccacctcga agatctcaag
     7021 atccggcaga tccgcggcca cgcgttggcc cagtgcgaga cgcacctgct ctcgcgtcgt
     7081 ccgatcatcg gagtagagca ggatcttggc gctctgttgg gtcatgcgga ttcctcgtct
     7141 acgggcgatc gaccggttcg tcagcatgct accccccggg gcgctgcaga ctcccaccgc
     7201 cccgcgccgc ccctcctaag aagcgtctcc tgaccctggc cgcgctgcgg cgcatccagt
     7261 acgcctgctc actgcgttgt catacccggc gagaggaccc gctatcggct cgtctcccgc
     7321 cttgtggtca gacgcactgg gcacgtcctc gcggaacgcc cagggttagg agacacctcc
     7381 tagcggaaga gctcgacgtg gtcggtgacc caggcccgga acgtccgtgc ggactggccg
     7441 gtgatccggg tgtactcgcc ggagatctcc gggcgggcgc cgacggactc ccgccaaccc
     7501 ttgattatct gcgacgacca ctcggtggtg acgaactgcg acagcagggc gctcgactcg
     7561 cgctcgctct gttctctcac cgagaccggg cggccgatca ccgaaccgat ggttgcgact
     7621 tgttcacgca ggctcaggcg ttcggggccg gtgagaaccg gcctcgcacc gaggagggtc
     7681 tcaccggtga gggcgaccac cgccacgtcg gcgatgtctg cctcgtggac gggcacctgc
     7741 acagcctccg ggtagggcag cggcacgacg ccggccgtct tcacgctcca actccaccgc
     7801 gcagcgttgc tcgcgaagct gcccgggcgc aggaaggtgt agtccagtcc tgagccgtcg
     7861 atcgcccgct cgacccggac gaacctcgcc ccgttgaagt cctcttccgc accggggaac
     7921 gtcaccgacg acgacgacag cagcaccgcc tgtcgaaccc cggaccgaac caactctgcg
     7981 accagcgcct cgggatcgcc gagatcggcg tacaggaaca cgcgctcgac tccctgaagc
     8041 gccggcccga acgaggccgg aacgttgaga tcggcggcga acacccccac ctgagggggc
     8101 agtgccaact tgcggggatc acgactcgtc gcgcgcaccg gcaccccctg cgcaaccagc
     8161 cccgcgacca cccgggcgcc caccgcgcca ctcgcaccga caaccaacac cgacattgac
     8221 ttccctttcg acgggaacac catagacgaa tggcgttcgc gaatcatgtt cgtcacgaat
     8281 catgtccgtg tcaagtgagt aggatgcggc catgacctcg cccgctgaac caatctctcg
     8341 ccgggatcga cccgccaagg caccgctgtc gagacggtcc atcctcgacg ccgcgatcga
     8401 actgatccgg gtccgggggg tcgccgcggt cacgctcaag gacgttgccg aggcggtgca
     8461 gaccggtccg gcctcgctgt acgcctactt tcggaaccgg gacgaacttc tcgagtgcgt
     8521 tctcgatgcc gcctacgccc aggtggaact cgtccacccg ctcgacggcg attggcgagg
     8581 cgcgctggcg gcgacgatcg tcaacacgat cgagaagctc gagagctttc ccgggctggc
     8641 cacagtcgcc ctcggcgcca ttcccaccag ggagggggcg ctgcgcctgg ccgagcacga
     8701 actcaccctg atggagctcg gcggcatcga cgcggacgct gctgccctgg gcgtggacct
     8761 gatcgcacag ttcgctgccg cgacggcgat cgagcgcgcc gtgcccggca agcgtgaacg
     8821 cggacacacc gaacgagaac agctgaaggc ggcctacggc ggcgccgacc cgcaggtgta
     8881 cccccgcgtc gcgaggctgg catccgccct caccgcaccg gacgaaccct cgcgtcgcga
     8941 cttcgcgatc caggccatcc tgaacggcct caccagcacg ccctacccac cgacccgccc
     9001 gaccccatga caagggattt cgcggcattt tcaggcggag gcttggtcgc ctgaacctgg
     9061 aggccacccg gccatcgcca ggcaggacct gccgctgagc atcaccctcg tgatcgacgg
     9121 cagaggacga acccgcccgc cagtggccgg cagaaggcac cccacgccgt cggactcacc
     9181 gtcgacggcc ggctccccgc ccccgcgctg cgggtggtca ccgactggtg cgacgaccca
     9241 acgcggtgct gagccccgtc ccagccacac gacaacgcag tgacacaaaa gcctgacggt
     9301 gcaggctgcg cgccccggct tgccgtggtc ctacgcgccc gacgcacgcc tcagccgctg
     9361 ctgcgcgacc accgtgcacg atcgggccgc cgccgaaccg gcgtcgtgcc catcgctcga
     9421 agtccatggc cgcaggatca caagtgtgtg acgagcgaga atcgcacccc tccagagggt
     9481 gaccaatcgg ctctgagcag caataatgac cgggtgactc actcgcacac gactgcggcc
     9541 ccggtgatgc cgagcggggc ggtgcacccg atcgcaccgg cccggctgct tccgcccaag
     9601 gggcgtcccg atctggttgc cgtcggcacc tggatctggc tggcgtccga cctgatgttc
     9661 ttcgcggcgc tgttcgcggc ctacttcaac ctgcgttcga tcaccaatgc cgcagccagc
     9721 gccgccggca ccgagtcgat gtggcaatgg ggcaccgcgc acttcaacct gcccttcgcc
     9781 gccaccaaca cgctgatcct gatcctgtcg tcggtgacct gccagatggg ggtctggaag
     9841 gccgagcgcg ggcaggtcgg acgcaccggc tcgctactgc aggtgggtca gtgggggttg
     9901 cgcgagtggt acatcctgac cttcatcatg ggcgcgatct tcgtcgccgg tcaggccacc
     9961 gaatacgcca cgttgatctc cgagggcttc accctgtcga ccaacgccta cacctccgcg
    10021 ttctttctgg ccaccggctt tcacggcctg cacgtcaccg gcggcctgat cgcattcctg
    10081 ttcatgctcg gacgcaccta tgtgagccgc accttcaccc acgagcaggc cgtccacgcc
    10141 gttgtcacct cgtactactg gcacttcgtc gacgcgatct ggatcattct gttcagcgtc
    10201 atctacatcc ttcgctgaac gacctgaccg agaggaaccc ctgtgcgatt cctgactgcc
    10261 aagcggcgac accgggccgc acggcccgcg ctgctggtgc tggccctgct gctgatgggc
    10321 gcgctgtacg cagctgtcgc accggccgca agagtgtcgg ccgacaccgg taacagccag
    10381 caggtggccg agggcaaagc cctgttccag gtgacctgct cgtcgtgcca cggcatgaac
    10441 ggcgaaggca ccagccaggg cccgtccctg gtcggcgtgg gggccgcttc ggcaaacttc
    10501 caggtcggca ccggccgcat gccgatggcg aacccgggtg cccaagcccc gcagaaacgt
    10561 accaactaca ccgccgacga gatcgcggca ttgtccgcct ttgtcgccag cctcggcccc
    10621 ggcccggcca ttcccaccga ggagcagtac agcggtgagg gcctcagcgc cgaagaactg
    10681 gccaagggcg gcgagttgtt ccgcaccaac tgttccgcct gccacaactt cgagggtgcc
    10741 ggcggtgcac tgcccaacgg caagtacgcg cccagccttc agggcgtcag cggccaacac
    10801 atctgggagg cgatgcgcac agggccacag cagatgccgg tcttcagtgc cggcgcactg
    10861 cccgatgaca acgtgcgggc catcgttggc tacctcgaga ccctgcacgc tcagccgtcg
    10921 agcggtctca ccttcggtgc tctcggcccc gttgccgagg gcctgttcgc ctggatcatc
    10981 ggcatcggcg ggctgtgcct gatcgccgtc tggatcgccg cgaggggagc acgagccaaa
    11041 tgagcatcga gacccgacag tcggaaacag aggtcgaacg gttccccgtt cccgaccccg
    11101 gccttgagcc gcacatcgaa cgctggaccg acgccgacaa gtccgccggt gatcgcgcgt
    11161 accgccaggt actgatgatg ctgggtgccg tcccggtgct cgcgatcgct ttcgtggtca
    11221 tctacttcac cgtgccgcgc accagcttcg tcgacatctt cggcatgcaa tgggacgcgc
    11281 tgacctttct gctcggtctc acgggcggcc tggccacact gctgatcggc ctggcctgca
    11341 ttcactgggc caagacgatg atgggcgacg aagagattca ggaggcccgc cactccgccg
    11401 cgtccagcgc tgaggaccgc gaggtcttcg cggccgagtt cgccaagggc gccgagcagt
    11461 cgggtctcgg tcgccgcaag ctgatcggcg gcgcattggg cggcgcgctc ggactgatcg
    11521 cggtgcccgc cgtggtgctg ttcgccgata tgggtccctg gcccactgct gcggtgcgca
    11581 aagagaccat cgagcgcacc atctgggcgc agggcgtcaa gctggtcaac gacatcaccc
    11641 ggcgtgcgct gaaggcgtcc gacctcgaga tcggccagtt ggtcaacgcg cagcccgaga
    11701 acctgtggga ccaccacggc accgacttcc aggtcgaaaa ggccaaggcg tcgatcatca
    11761 tcgtgcgcat ggaccccacg cagatcaaga tccccgaatc gcgcaaggac tggcaggtcg
    11821 gcggcatcct ttgctactcg aagatctgca cccacgttgg ttgcccgatc agcctgtggg
    11881 agcagcagac ccaccacctg ctctgcccct gccaccagtc cacgttcgac ctgtcgaact
    11941 cgggcgtggt ggtgttcgga ccggcggcgc gatcgctgcc ccagttgccg atcacggtcg
    12001 atgccgacgg ttacctggtc gcacgcagcg acttcacggt gccggtcggt ccgagcttct
    12061 tcgagcgtga ctcgaaccac gatttcgtag agggtgatcg gtgatggcca agcccgcgaa
    12121 cgtctccacc gagtccccgg tgctcgacac cgaacgcccg cccaagccgg acgggctgcc
    12181 gtccgcggtc accggcgcgg ccacctgggc ggacgaacgc ctgggcctgg cgagtctgtt
    12241 gaagaagaac ctgcgcaagg tgtttcccga ccactggtcg ttcctgctcg gcgagatcgc
    12301 tctgtactcg ttcctcatcc tgctcctcac cggtgttttc ctgaccatct ggttcaagcc
    12361 gtccatggcc gagatcgagt accagggcag ctatcagtta atgcgcgggt tgcccatgtc
    12421 cgaggcgttc gcctcgacgc tcaacttgtc gttcgatgtt cgcggcggac tgctggtgcg
    12481 ccagatccac cactgggcgg ccatgttgtt catcgccgcg atgaccgtgc acatgctgcg
    12541 tatcttcttc accggcgctt tccgcaagcc gcgcgagatc aactggctga tcggcgtggg
    12601 cctgttctcg atgtcgttga tcgagggctt cgccggctac tcgctgcccg atgacctgct
    12661 ctcgggcacc ggcctgcggt tcgtggacgg cctcatccgc tccattccgc tggtcggcac
    12721 ctggctcgag ttcttcatct tcaacggtga gtttcccggc gacctggtca ttccgcggct
    12781 ctacatggtg cacattctgc tggtgccagg gctgatgctc ggcctgatcg cggcccatct
    12841 cgcgctggtg gtgtaccaca agcacacgca ctaccccggt ccgggccgca aggacggcaa
    12901 cgtcgtcggc tacccgctgt tcccggtgta catggccaag gccggcggct tcttcttcgt
    12961 ggtcttcgga gtgtcgatcc tgatgggtgc cacgatgcag atcaacccgg tgtggaccgt
    13021 cggtccgtac aacccggcac aggtcagcgc gggctcgcag cccgactggt acatgggctg
    13081 ggtcgagggc gcggtccgga tcatgccgaa ctgggaatgg catctgggca gcaccacc
//
LOCUS       DAOSUG010000014        12956 bp    DNA     linear   ENV 10-JUN-2024
DEFINITION  MAG TPA_asm: Micropruina sp. isolate AMR_MDS_2195
            NODE_2855_length_12956_cov_4.300674, whole genome shotgun sequence.
ACCESSION   DAOSUG010000014 DAOSUG010000000
VERSION     DAOSUG010000014.1
DBLINK      BioProject: PRJNA925477
            BioSample: SAMN32864262
            Sequence Read Archive: SRR4236673
KEYWORDS    WGS; ENV; Metagenome Assembled Genome; MAG; Third Party Data; TPA;
            TPA:assembly.
SOURCE      Micropruina sp. (wastewater metagenome)
  ORGANISM  Micropruina sp.
            Bacteria; Bacillati; Actinomycetota; Actinomycetes;
            Propionibacteriales; Nocardioidaceae; Micropruina.
REFERENCE   1  (bases 1 to 12956)
  AUTHORS   Abdulkadir,N., Saraiva,J.P., Zhang,J., Stolte,S., Gillor,O.,
            Harms,H. and Rocha,U.
  TITLE     Genome-centric analyses of 165 metagenomes show that mobile genetic
            elements are crucial for the transmission of antimicrobial
            resistance genes to pathogens in activated sludge and wastewater
  JOURNAL   Microbiol Spectr 12 (3), e0291823 (2024)
   PUBMED   38289113
REFERENCE   2  (bases 1 to 12956)
  AUTHORS   Abdulkadir,N.
  TITLE     Direct Submission
  JOURNAL   Submitted (24-MAR-2023) Environmental Microbiology, Helmholtz
            Center for Environmental Research -UFZ, Permoserstr 15, Leipzig
            04318, Germany
COMMENT     The annotation was added by the NCBI Prokaryotic Genome Annotation
            Pipeline (PGAP). Information about PGAP can be found here:
            https://www.ncbi.nlm.nih.gov/genome/annotation_prok/
            
            ##Genome-Assembly-Data-START##
            Assembly Method        :: SPAdes v. 3.11.1
            Genome Representation  :: Full
            Expected Final Version :: Yes
            Genome Coverage        :: Not Applicable
            Sequencing Technology  :: Illumina MiSeq
            ##Genome-Assembly-Data-END##
            
            ##Genome-Annotation-Data-START##
            Annotation Provider               :: NCBI
            Annotation Date                   :: 05/04/2023 04:57:10
            Annotation Pipeline               :: NCBI Prokaryotic Genome
                                                 Annotation Pipeline (PGAP)
            Annotation Method                 :: Best-placed reference protein
                                                 set; GeneMarkS-2+
            Annotation Software revision      :: 6.5
            Features Annotated                :: Gene; CDS; rRNA; tRNA; ncRNA
            Genes (total)                     :: 2,776
            CDSs (total)                      :: 2,750
            Genes (coding)                    :: 2,714
            CDSs (with protein)               :: 2,714
            Genes (RNA)                       :: 26
            tRNAs                             :: 24
            ncRNAs                            :: 2
            Pseudo Genes (total)              :: 36
            CDSs (without protein)            :: 36
            Pseudo Genes (ambiguous residues) :: 0 of 36
            Pseudo Genes (frameshifted)       :: 2 of 36
            Pseudo Genes (incomplete)         :: 32 of 36
            Pseudo Genes (internal stop)      :: 2 of 36
            ##Genome-Annotation-Data-END##
FEATURES             Location/Qualifiers
     source          1..12956
                     /organism="Micropruina sp."
                     /mol_type="genomic DNA"
                     /submitter_seqid="NODE_2855_length_12956_cov_4.300674"
                     /isolate="AMR_MDS_2195"
                     /isolation_source="Activated sludge of municipal
                     wastewater treatment plants"
                     /db_xref="taxon:2737536"
                     /environmental_sample
                     /geo_loc_name="Canada"
                     /lat_lon="49.25 N 123.1 W"
                     /collection_date="2015-06-16"
                     /metagenome_source="wastewater metagenome"
                     /note="metagenomic"
     gene            <1..601
                     /locus_tag="PLF56_00945"
     CDS             <1..601
                     /locus_tag="PLF56_00945"
                     /inference="COORDINATES: protein motif:HMM:NF019310.2"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_function: GO:0022857 - transmembrane transporter
                     activity [Evidence IEA];
                     GO_process: GO:0055085 - transmembrane transport [Evidence
                     IEA]"
                     /codon_start=2
                     /transl_table=11
                     /product="MFS transporter"
                     /protein_id="HOQ52159.1"
                     /translation="LAMMALFGTVIMLPLILQQALHLPPLTVGLMLLPGGLLMGVLGP
                     VSGRLYDRVGPRPLMLPASVAVAFAFWLLSTISLNTPVWFVVACHVVMSASFAFMFTP
                     LFTIALGSLPRQLYSHGSAIVGTVQQVAGAFGTAVFVTVFASQSALARDAGASVEAAL
                     LQGSHFAFLSAGAVWTLAIVATAFIKPPDAGDESAPPLH"
     gene            766..1617
                     /gene="ppk2"
                     /locus_tag="PLF56_00950"
     CDS             766..1617
                     /gene="ppk2"
                     /locus_tag="PLF56_00950"
                     /EC_number="2.7.4.1"
                     /inference="COORDINATES: protein motif:HMM:TIGR03707.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_component: GO:0005575 - cellular_component [Evidence
                     IEA];
                     GO_function: GO:0008976 - polyphosphate kinase activity
                     [Evidence IEA];
                     GO_process: GO:0006793 - phosphorus metabolic process
                     [Evidence IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="polyphosphate kinase 2"
                     /protein_id="HOQ52160.1"
                     /translation="MTKAPKLDNDLYEGELHRLQAELVDMQEWIRQEGQRLVVIFEGR
                     DAAGKGGAIKRITEYLNPRVAQVVALPAPTERQQTEWYFQRYIQHLPSAGQIKLFDRS
                     WYNRAGVERVMGYCTTAEHRRFLRQCPIVERMLMEDGILLRKYWFSVSDKQQEKRFKS
                     RLTDPMRRWKLSPTDLESLTRWEDYSRAKDEMFVHTDIPEARWNVVEAEDKKRARINM
                     IAHLLSSIPYEKIDPPLLKLPKRPASTGYQRTDRSLQYEVPDHAAGLVLDHDAVRYVD
                     PEDDDSE"
     gene            1701..2357
                     /locus_tag="PLF56_00955"
     CDS             1701..2357
                     /locus_tag="PLF56_00955"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_017936438.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_function: GO:0003677 - DNA binding [Evidence IEA];
                     GO_process: GO:0000160 - phosphorelay signal transduction
                     system [Evidence IEA];
                     GO_process: GO:0006355 - regulation of DNA-templated
                     transcription [Evidence IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="response regulator transcription factor"
                     /protein_id="HOQ52161.1"
                     /translation="MLNKPPIRVALVNDYEVVVAGLLHMFANYPERIEVVKLVANREV
                     RERVDIALYDSFGQTSSEAVAIEHVLHNPLVRRTVVYTWNFQLARIDQTTHLGASGYL
                     SKTLPARELVDALEQVHAGKLVVSPAPKSATPTGGNWPGREEGLSYREGEVVALITQG
                     LSNAEIASTMYLSPNSVKSYIRSAYRKIGVARRSQAVSWGMRHGFAADYVRLDPDDVA
                     "
     gene            complement(2392..3015)
                     /locus_tag="PLF56_00960"
     CDS             complement(2392..3015)
                     /locus_tag="PLF56_00960"
                     /EC_number="2.7.7.87"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_016669940.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_function: GO:0061710 - L-threonylcarbamoyladenylate
                     synthase [Evidence IEA];
                     GO_process: GO:0006400 - tRNA modification [Evidence IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="L-threonylcarbamoyladenylate synthase"
                     /protein_id="HOQ52162.1"
                     /translation="MARYFDVHPDNPQPRALGQVTRILRDGGLIAYPTDSGYALGCML
                     GNRAGLDRIRAIRQLDAKHNFTLVCSEFAQLGQYVQMSNDVFRAIKAATPGGFTFILT
                     ATREAPRVMLQPKRRTVGVRIPEHTTALALLHELGEPLMSSTLLLPGHDEPMSDGWTV
                     NDEIGHALDAVLDSGDCGTEPTTVIDLSGDEVVIVRRGSGDPELFEE"
     gene            3110..4237
                     /locus_tag="PLF56_00965"
     CDS             3110..4237
                     /locus_tag="PLF56_00965"
                     /inference="COORDINATES: protein motif:HMM:NF012382.2"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_function: GO:0030170 - pyridoxal phosphate binding
                     [Evidence IEA];
                     GO_process: GO:0009058 - biosynthetic process [Evidence
                     IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="aminotransferase class I/II-fold pyridoxal
                     phosphate-dependent enzyme"
                     /protein_id="HOQ52163.1"
                     /translation="MTIFSLTESELRQRRSLKWRTYAPDVLPLWVAEMDAAVQPSVRR
                     ELDAALDRGDTGYPWGNDYADAYRASAASRWGLELDPAQVRRGGDVMNAVLCILEKVT
                     DPGDGIVITAPVYPPFWQVTSGYRRRTVAVELTAEGRLDLPALEQAFAAPDVTAFLLC
                     SPHNPTGTVHSAEELAAVATLCERNGVRLVVDEIHAWLVDPGTMFVPILSLPEASRAL
                     VAISAGKSWNLAAFKAGLYVGGADADDVLDHLPPLATQSTGHLGAIAHAAAVRNEQAW
                     VDQVCAEVAANKQLLSDLLHALLPAAQYRAAPGTYLAWVDCTELGLGNPARHFEQVGR
                     VAFSPGANFSQAHDQWVRVNLATSPQILTQAVERMAVSLRA"
     gene            complement(4267..4680)
                     /locus_tag="PLF56_00970"
     CDS             complement(4267..4680)
                     /locus_tag="PLF56_00970"
                     /inference="COORDINATES: ab initio
                     prediction:GeneMarkS-2+"
                     /note="Derived by automated computational analysis using
                     gene prediction method: GeneMarkS-2+."
                     /codon_start=1
                     /transl_table=11
                     /product="pilus assembly protein TadE"
                     /protein_id="HOQ52164.1"
                     /translation="MRQPTSSPRRSERGMALSSFVVVLVIALLLVAGLVVDGSRKSAA
                     DRRAELTAAAAARSAVDETAAERQAGRRPNASAAIAAANRVIADEAGMHGSAALAADG
                     SVQVTTSTSVDTVFLSLIGIRSLQGHGSARAQLFD"
     gene            4826..6052
                     /locus_tag="PLF56_00975"
     CDS             4826..6052
                     /locus_tag="PLF56_00975"
                     /EC_number="2.7.1.90"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_013161100.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_process: GO:0006002 - fructose 6-phosphate metabolic
                     process [Evidence IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="pyrophosphate--fructose-6-phosphate
                     1-phosphotransferase"
                     /protein_id="HOQ52165.1"
                     /translation="MVHKVALLTAGGFAPCLSSAIGGLIQRYTEVSPDTEIIAYRHGY
                     QGLLTGDYLSVTPEVRAKAHLLHQYGGSPVGNSRVKLTNAKDLVKRGLVAEGDDPLKV
                     AAERLVADGVDVLHTIGGDDTNTTAADLAAYLAKHDYGLTVVGLPKTIDNDVIPIRQS
                     LGADTAAEMGARFSRNVLAEHNAGSRLLIVHEVMGRNCGWLTAATARKYREWIAQAEW
                     LPEIGLDAKAWDVHAVFVPEAVIDLEAEAARLHEVMDSLGNVNIFVSEGAGVESIVAE
                     MEKAGQEVPRDAFGHIRLDKVNPGAWFGQQFAQMLDAEKTLVQKSGYYARSAAANQFD
                     LDLIHSMTDLAVDTALRGESGVIGHDEENGDVLTAIAFDRIKGGKPFDLTQQWYLDLL
                     SEIGQPAPVAAPPAEH"
     gene            complement(6218..7084)
                     /locus_tag="PLF56_00980"
     CDS             complement(6218..7084)
                     /locus_tag="PLF56_00980"
                     /inference="COORDINATES: protein motif:HMM:NF017085.2"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="CHAD domain-containing protein"
                     /protein_id="HOQ52166.1"
                     /translation="MNAATRAVLDYLRTQADVIATRADDVLIDAPDAVHRSRVATRRT
                     RSALRTFTPLFKKTRTRALRDELVWHADRLGAPRDAEVLKERLLATLDALPSDHVVGP
                     VRARLADELDLAHAAAHAQLVVSMQTTRYADLRAELNDFVTQPPIGKGGATLGKQHLA
                     DLHTLAIDRTRRLYVRSEEVPGDLHMWHEVRKAAKAARYCSEALTQAFGEAAEERARA
                     WEQVTEALGEVQDTVVAEQTLIRHAATALSAGEPVETYLALESVELRQREQSLATGRR
                     VLAEALLLPPLR"
     gene            complement(7081..7755)
                     /locus_tag="PLF56_00985"
     CDS             complement(7081..7755)
                     /locus_tag="PLF56_00985"
                     /inference="COORDINATES: ab initio
                     prediction:GeneMarkS-2+"
                     /note="Derived by automated computational analysis using
                     gene prediction method: GeneMarkS-2+."
                     /codon_start=1
                     /transl_table=11
                     /product="hypothetical protein"
                     /protein_id="HOQ52167.1"
                     /translation="MTSTSSPATVEPVPERVRHGDAWSASMFGVAALVVGIVAAVIWR
                     LVVRLPSYVVQSDGSANVSERALTEFFAADAWYVVLGVVFGAIIGIFTWRRFKTLGWV
                     SAFLAVGLGALAGVVCWQLGQLFVGAPFDERLASAQPGDQVPIALALHSPSALAVWAF
                     AAVTPILLGSALGPDEEAGPHEPRRRRRQVEPPPEDQGEVIDELGVVRPDEATVARNE
                     EPGGTS"
     gene            complement(7793..11305)
                     /gene="dnaE"
                     /locus_tag="PLF56_00990"
     CDS             complement(7793..11305)
                     /gene="dnaE"
                     /locus_tag="PLF56_00990"
                     /EC_number="2.7.7.7"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_013863754.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_function: GO:0003676 - nucleic acid binding [Evidence
                     IEA];
                     GO_function: GO:0008408 - 3'-5' exonuclease activity
                     [Evidence IEA];
                     GO_process: GO:0006260 - DNA replication [Evidence IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="DNA polymerase III subunit alpha"
                     /protein_id="HOQ52168.1"
                     /translation="MADGFVHLHCHSEYSMLDGAARLKDLVKGCEQMNMPALAVTDHG
                     NLFGAYEWYKVAKGSAVKPIIGLEAYLTPKTHRSERKRVQFGDGTGDDVASKGAYTHM
                     TMWAQNSEGMHNLFRLSSLSSLEGFFYKPRADRELLHRYGKGLLATTGCPSGEVQTYL
                     RLGQYDQALESAAEFRDIFGAGNFYCELMDHGLDIERRVRHDLLRIAKDLKLPLVATN
                     DLHYVHADDAAAHDTLLCVSSGSNRDTPNRFKFDGNGYYLKSPAEMRQIWAELPEACD
                     NTLAIAERCSTHFDEGTGTYMAQFPVPAGETEESWFVKEVRRGLEARFPEGVPSYATE
                     QAEFETTVIAGKGYSGYFLVVADFINWAKSNGIRVGPGRGSGAGSMCAYALRITDLDP
                     VPHGLIFERFLNPERPSMPDFDVDFDERRRGEVIRYVTEKYGDDHVCQIVTYGTIKAK
                     QAIKDAGRVLGKPFGLGEKITKAYTPPVMGKDVPLPEVFNPKHARYAEGGEFRALYES
                     DPEVQEVVDTARGIEGLKRQWGVHAAGVIMSSAPLMDVIPVMKREADGQIITQFDYPS
                     CEALGLVKMDFLGLRNLTILDDAIANVKANRGLDLDLDELSKNPTDPATYQLLASGDT
                     LGIFQLDGTGMRVLLRQMQPDNFEDISAALALYRPGPMGANSHTNYALRKTGKQPITA
                     IHPELAEPLAEILDLTYGLIVYQEQVQQIAVKVANYTLGAADQLRRAMGKKKKEVLDA
                     EFVHFEAGMQANGFSAGAIKSLWDILVPFSDYAFNKAHTAAYGLISYWTAYLKANYPA
                     EYMAALLQSVKDDKDKSAIYLAECRRMGIKVLAPDVNESDGMFTPVGTDIRYGLGAIR
                     NVGDNVVKGIVEGRDEHGKARDFNEFLSNVPLVVCNKRVIESLIKAGAFDSLGHTRRS
                     LMECFEQKVDEVIDLKRNQANGQDDLFGDPAGGEAVVSSPIPEIDEWDKRVRLAFERE
                     MLGLYVSDHPLLGLEHVLLANRDHSIGAIVAEDGPRDITTAIAGMITGVNRKQTKNGD
                     LWAIVTVEDLEASVEVLLFPKAYQQVALQLATDTIVRIKGRVVGKDEGVELRGTDVSF
                     PDVSEGPSGPVVISLPAARCTPAVVQQLRTVLTIHPGVTEVRVRLTTSSNRTTVWKLE
                     ETLRVMPSPPLMADLKALLGPSCV"
     gene            complement(11463..12722)
                     /locus_tag="PLF56_00995"
     CDS             complement(11463..12722)
                     /locus_tag="PLF56_00995"
                     /EC_number="3.1.1.103"
                     /inference="COORDINATES: protein motif:HMM:NF012371.2"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_function: GO:0016787 - hydrolase activity [Evidence
                     IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="serine hydrolase domain-containing protein"
                     /protein_id="HOQ52169.1"
                     /translation="MSSELYTPVDPEEVGVSSQYLANIDALQQRYLAENAYQGSVVLV
                     ARHCKVAYFKAFGKADDDVEMATDSIFRLASMSKVVSAVAVLQLFEQGRIALHEPIAT
                     YLPEFADTKVAVVKDWAVTQLRTASRPITIHDLLTMTAGMTNTWWHGLFEPPIYHVVP
                     KLYAEAGLIDDLRTPPTTLEENTRLIASLPLIADPGTMFDYSNNSVDVLCRLVEVVSG
                     QDFDSYLRQHIFQPLRMNETWFFPPQDQWHRIAAVYWAGRDEKQTDTSPLGLGHLGPN
                     YTFSDDRTLFSGAGGLHGTTADYFRFAQMLLNQGELDGVRVLSPASVRLMTTNQIGDL
                     TNWQLTQNKWGYMVDLQEGENAPAGSIHWLGGPGAYSWQGFFSTKFVNNPRLDTVILT
                     MSTPGFDGALPHNLRLVAAATAAVVGD"
BASE COUNT         2016 a         4290 c         4448 g         2202 t
ORIGIN      
        1 actggccatg atggcgctgt tcggcaccgt gatcatgctg ccgctgattc tgcagcaggc
       61 cctgcacctg ccaccactga ccgtcggcct gatgctgctg ccgggtgggt tgttgatggg
      121 cgtgctcggt ccggtcagcg gacgcctgta cgaccgtgtc ggtccgcggc cgttgatgct
      181 gcccgccagc gtggcggtgg cgttcgcctt ctggctgctg tcgacgatct cgctgaatac
      241 cccggtgtgg ttcgtggtgg cctgtcacgt ggtgatgagc gcgtcgttcg ccttcatgtt
      301 cactcccctg ttcacgattg cgctgggctc gttgccccgt cagctctact cccacgggtc
      361 ggcgatcgtc ggcaccgtgc agcaggtggc cggcgcgttc ggcaccgcgg tgttcgtcac
      421 cgtgttcgcg tcgcagtcgg cgctggcgcg ggacgccggc gcaagcgtcg aggcagccct
      481 gttgcagggc tcgcacttcg cgtttctcag cgccggcgcc gtgtggacgc tcgccatcgt
      541 cgccactgcg ttcatcaagc ccccggacgc cggcgacgaa tcggcaccgc cgctgcactg
      601 acgaatgcgt cggcgacgcg agtctgcagg cgcaacgccc tcgccggtcc tcacagacgc
      661 catgggcccg cccccgccga atctcgggtt gtgcgacacc ccccctagtg ggacgacttt
      721 tgcggggcgc tggtggggcg gcggggtttg gtgcacgata cgtccatgac gaaagcgccg
      781 aagctcgaca atgacctcta cgagggcgag ttgcatcgtc ttcaggccga gctcgttgac
      841 atgcaggagt ggatccggca ggagggccag cggctcgtcg tgatcttcga gggacgggac
      901 gcagccggca agggtggcgc gatcaagcgc atcaccgagt acctcaaccc gcgcgtcgct
      961 caggtggtcg cgctgccggc gccgaccgag cggcagcaga ccgagtggta ctttcagcgc
     1021 tacatccagc acctgccctc ggccggccag atcaagctgt tcgaccgctc gtggtacaac
     1081 cgggccggcg tcgaacgggt gatgggttac tgcaccaccg ccgagcaccg ccgctttctg
     1141 cggcaatgcc cgatcgtcga gcgcatgctg atggaggacg ggatcctgct gcgcaagtac
     1201 tggttctcgg tgtcggacaa gcagcaggag aagcggttca agtcgcggct gaccgatccc
     1261 atgcggcgct ggaagctctc accgaccgac ttggagtcat tgacccgttg ggaggactat
     1321 tcgcgggcca aggacgagat gttcgtgcac accgacattc cagaggcgcg ctggaacgtg
     1381 gtggaggccg aagacaagaa gcgggcccgg atcaacatga tcgcgcacct gttgtcgtcg
     1441 atcccctacg agaagatcga cccgccgttg ctcaagttgc ccaagcgccc ggcgtccacc
     1501 gggtaccagc gcaccgatcg cagcctgcag tacgaggtgc ccgatcatgc ggccggactg
     1561 gtgcttgatc acgacgcggt gcgctacgtc gaccccgagg acgacgacag cgagtagccc
     1621 cgagtagtcc gtaggtggag ctgagcccca aagggtggtg acctgccccc cttcgggtga
     1681 ttcagtggaa tactgggggt gtgctgaaca aaccaccgat ccgcgtcgcc ctggtgaacg
     1741 actacgaagt cgtcgtcgcc ggcctgttgc acatgttcgc caactacccc gagcgcatcg
     1801 aggtggtaaa gctggttgcc aaccgtgaag tacgcgagcg ggtcgacatc gcgctctacg
     1861 acagcttcgg gcagacctcg tccgaagcgg tcgccatcga gcacgtgctg cacaatccgc
     1921 tggtcaggcg cacggtcgtc tacacctgga actttcagct ggcgcgcatc gaccagacca
     1981 cgcatctggg tgcgtccggc tacctgtcca agacgctgcc cgcccgtgag ttggtggacg
     2041 cgctcgagca ggtgcacgcc ggcaagctgg tggtcagccc cgcgcccaag tcggccacgc
     2101 ccaccggagg caactggccg ggtcgcgaag aggggctctc gtaccgcgag ggtgaggtgg
     2161 tcgcactgat cacgcaaggc ctctccaacg ccgagatcgc ctcgacgatg tacttgagcc
     2221 cgaactcggt gaagagctac attcgctcgg cgtaccgcaa gatcggcgtg gcccggcgtt
     2281 cacaggcggt gtcatggggc atgcgccatg gtttcgcggc tgactacgtg cgactcgacc
     2341 ccgacgatgt ggcctgagcc cggtgcggct tcggcctgag tgaactcgcg gctactcctc
     2401 gaacaattcg ggatcgcccg atccgcgccg gacgatgacc acctcgtcgc cgctcaggtc
     2461 gatcaccgtg gtcggctcgg tgccgcaatc gcctgagtcg agcaccgcgt ccagggcgtg
     2521 tccgatctcg tcgttgacgg tccacccgtc cgacatcggc tcgtcgtggc cgggcagcag
     2581 cagcgtgctc gacatcagcg gctcacccag ttcgtgcagc agggccagtg cggtggtgtg
     2641 ctcgggaatg cgcaccccga cggtgcggcg cttcggctgc agcatcaccc ggggagcctc
     2701 gcgggtggcg gtcagaatga aggtgaaccc gcccggtgtg gcggccttga tggcgcggaa
     2761 gacgtcgttg ctcatctgca cgtactgacc cagctgggcg aactcgctgc acaccagcgt
     2821 gaagttgtgc ttggcatcga gctggcggat ggctcgaatg cgatccaggc cggcccggtt
     2881 gcccagcatg cagcccagcg cgtagccgga gtccgtcggg taggcgatca atcccccgtc
     2941 gcgcagaatc cgggtgacct gacccagcgc cctcggctgc ggattgtcgg ggtgcacgtc
     3001 gaaatacctg gccatcgctt cgttgcctcc gatcagcgcg gacgacggga tgactcgacc
     3061 ctagtcgaga ctcggcgctc ggggcgcccc gagccgcaac aatgcagcca tgacgatctt
     3121 ctccctgacc gagagtgaac tgcggcagcg gcgcagtctg aagtggcgca cgtacgcccc
     3181 ggacgtgctg ccgctgtggg tcgccgagat ggacgccgcg gtgcagccgt cggtgcgtcg
     3241 cgagctggac gcggcactgg atcgcggcga caccgggtac ccctggggca acgactacgc
     3301 cgacgcctac cgggcctcgg ccgcgtcccg ctggggcctg gagctcgatc ctgcgcaggt
     3361 gcgtcgcggt ggcgacgtga tgaacgccgt gttgtgcatt ctcgagaagg tgaccgatcc
     3421 gggcgacggc atcgtgatca ccgcgccggt ctatccgccg ttctggcagg tgaccagcgg
     3481 ctaccggcgc cgtaccgtcg cggtcgagct caccgccgag ggccggctcg acctgccggc
     3541 cctcgagcag gccttcgccg cccccgacgt gacggccttt ctgttgtgct caccgcacaa
     3601 cccgaccgga acggtgcaca gcgccgagga actcgctgcg gtggccacct tgtgtgagcg
     3661 caacggcgtg cggctcgtgg tggacgagat tcacgcctgg ctggtcgatc ccggcacgat
     3721 gttcgtgccg attctgagcc tgcccgaggc cagtcgtgcg ctggtggcga tctcggccgg
     3781 caagtcgtgg aacctggccg ccttcaaggc gggcctgtac gtgggtggtg cggatgccga
     3841 cgacgtgctc gaccacctgc ccccgctggc cacccagtcg accggccacc tgggtgcgat
     3901 tgcgcacgcg gcggcggtgc gcaacgagca ggcctgggtc gatcaggtgt gtgccgaggt
     3961 ggccgccaac aagcaactgc tcagcgatct gctgcacgca ctactgccgg cggcgcagta
     4021 tcgtgccgcc cccggcacgt acctggcctg ggtcgactgc accgaattgg gcctgggcaa
     4081 cccggcgcgc cacttcgagc aggtcggccg ggtggccttc tcgcccggcg ccaacttctc
     4141 ccaagcgcac gaccagtggg tgcgggtgaa cctggccacc tccccgcaga ttctcactca
     4201 ggcggtggag cgcatggcgg tgtcattgcg cgcgtagcag gtcatggtgc gcgtggcggg
     4261 ccgggctcag tcgaagagtt gcgcccgggc gctgccgtga ccctgcagac tgcggatgcc
     4321 gatcaggctc agaaagaccg tgtcgacgct ggtgctggtg gtgacctgca cgctgccgtc
     4381 ggccgccagc gcggccgacc cgtgcatgcc ggcctcgtcg gcgatcaccc ggttggcggc
     4441 cgcgatcgcc gcgctcgcgt tgggacgacg tccggcctgt cgttcggctg ccgtttcatc
     4501 gaccgccgag cgtgccgctg ccgccgcggt cagttcggcg cggcggtcgg cggcgctctt
     4561 gcgcgacccg tccacgacga gtccagcgac cagtagcagg gcaatcacca ggaccaccac
     4621 gaacgacgac aacgccatgc ctcgttcgct gcgccgaggg gacgaggtgg gctgccgcat
     4681 cgccccagtg tgcaccggcc ggcatgccgg tgggtggttg tccacagagg tgcctgcgca
     4741 acccgtccgg ttgccggtgt tgcttccgcg gcgcgagcag gggcaatagg ctgaccgtgt
     4801 tcaaccccaa gcgagaggct ttttcatggt tcacaaggtc gccctgctca ccgccggcgg
     4861 tttcgccccg tgcctgtcgt cggcgatcgg cggtctgatc cagcggtaca ccgaggtctc
     4921 acccgacacc gagatcattg cctaccgcca cggctaccag ggcctactga ccggtgacta
     4981 cctcagcgtg acccctgagg tccgcgccaa ggcccacctg ctgcaccagt acggcggttc
     5041 gcccgtcggc aactcgcggg tgaagctgac caacgccaag gatctggtca agcgcggtct
     5101 ggtcgccgaa ggcgacgacc cgctgaaggt ggccgccgag cgcctggtcg cggatggtgt
     5161 cgacgtgctg cacaccatcg gcggcgacga caccaacacc accgccgccg acctggcggc
     5221 ctacctggcc aagcacgatt acgggctgac cgtggtgggt ctgccgaaga cgatcgataa
     5281 cgacgtgatc ccgatccggc agtccctggg tgcagacacc gccgccgaga tgggtgcccg
     5341 gttcagccgc aacgtgctgg ccgagcacaa cgcgggctcg cgcctgctga tcgtccacga
     5401 ggtgatggga cgcaactgcg gctggctgac cgccgccacg gcacgcaagt accgcgagtg
     5461 gatcgcccag gccgaatggc tgcccgagat cggcttggac gccaaggcct gggacgtgca
     5521 cgcggtgttc gtgccggagg cggtgatcga cctcgaggcc gaagcggccc gcttgcacga
     5581 ggtgatggac agcctcggca acgtgaacat cttcgtcagc gagggtgccg gggtggagag
     5641 catcgtggcc gagatggaga aggccggcca ggaggtgccc cgggacgcct tcggccacat
     5701 tcggctcgac aaggtcaacc cgggcgcgtg gttcggtcag cagttcgcgc agatgctgga
     5761 cgccgagaag acgctggtgc agaagtccgg ctactacgcg cgttcggcag ccgccaacca
     5821 gttcgacctc gacctgatcc actcgatgac cgaccttgcc gtcgacaccg cgctgcgcgg
     5881 cgaatcgggc gtgatcggtc acgacgaaga gaacggcgac gtgctgaccg cgatcgcttt
     5941 cgaccgtatc aagggcggta agccgttcga cctcacccag cagtggtacc tcgacctgct
     6001 gtccgagatc gggcagccgg cgccggtcgc ggcgccgccc gcggagcact gagttcgaca
     6061 cgctgtcagt tgcgccgtca tcccagcggg gtggcggcgc atcgtgcgtt ccgaaccggt
     6121 agtcagcggc cagcagggta gctcgtgaaa aaagggaatg gggaaccgtc cccacaccgt
     6181 tcggcgctgc gacggttgcg ggtgtccgcg cgccgggtca gcgcaggggc ggcagcagca
     6241 gcgcctcggc cagcacccgg cgtccggtgg ccagtgactg ctcgcgttgg cgcaactcga
     6301 cgctctcgag ggccaggtag gtctcgacgg gctcccccgc actcaaggcg gtcgcggcgt
     6361 gccggatcag ggtctgttcg gccaccacgg tgtcctgcac ctcgcccaga gcctcggtga
     6421 cctgctccca ggcccgggcc cgctcctcgg cggcctcgcc gaaggcctgc gtcagggcct
     6481 cggagcagta gcgcgccgcc ttggccgcct tgcgcacctc gtgccacatg tgcaggtcgc
     6541 cgggcacctc ttctgagcgt acgtacagcc gccgggtgcg atcgatcgcc agcgtatgca
     6601 gatcggccag gtgctgtttg cccagcgtcg cgccgccttt gccgatcggt ggctgggtga
     6661 cgaagtcgtt cagttcggca cgcaggtcgg cgtagcgggt ggtctgcatc gacaccacga
     6721 gttgggcatg tgccgcagcg tgagcgagat cgagttcgtc cgcgagccgc gcgcgcaccg
     6781 ggcccaccac gtgatcggac ggcagtgcgt ccagggtggc gagcagcctc tccttcaaca
     6841 cctcggcgtc gcgcggcgca cccagccggt cggcgtgcca gaccaactcg tcgcgcagtg
     6901 ccctggttcg cgtcttcttg aacaggggtg tgaaggtgcg cagggcacta cgggtgcggc
     6961 gggtcgcgac ccggctgcgg tgcacggcat ccggagcgtc gatcagcacg tcgtcggcgc
     7021 gggtggcgat cacgtccgcc tgcgtgcgca ggtagtcgag cacggcgcgc gtggctgcgt
     7081 tcatgaggtg ccgccgggtt cttcattgcg ggccacggtg gcctcgtcgg ggcggacgac
     7141 gcccagttcg tcgatcacct cgccctgatc ctcgggcggc ggctccacct gccttcgccg
     7201 tctacgcggt tcgtgcgggc cggcctcctc gtcgggcccc aacgccgaac cgagcagaat
     7261 gggtgtgacg gcggcgaagg cccacacggc cagggctgac ggcgagtgca gcgccagtgc
     7321 gatcggcacc tggtcgccgg gctgcgcaga ggccagccgc tcatcgaacg gcgccccaac
     7381 gaacagttgg cccagctgcc agcagaccac gccggccagc gcgcccaggc cgaccgccag
     7441 gaaagccgac acccagccga gcgtcttgaa ccgacgccag gtgaaaatgc cgatgatcgc
     7501 cccgaacacc acgcccagca cgacgtacca ggcgtcggct gcgaagaact cggtcagggc
     7561 ccgctccgac acgttggccg acccgtccga ctgcacgacg taactcggca gccgcaccac
     7621 cagccgccag atcaccgcgg ccacgatgcc caccaccagc gcagccaccc cgaacatgga
     7681 cgccgaccac gcatcgccgt gccgcacccg ctcgggcaca ggttccacgg tcgccgggga
     7741 cgaagtgctg gtcactcccc cagcctaatg gggagattcg tgtgggggtc cgctacacgc
     7801 acgacggccc gagcagcgcc ttgaggtcgg ccatcagcgg cggcgagggc atcacccgca
     7861 gtgtctcctc cagcttccac accgtggtgc ggttgctcga ggtggtcagc cgcacccgca
     7921 cctcggtgac gccggggtga atggtcagga cggtgcgcag ttgctgcacg acagccgggg
     7981 tacagcgggc cgcgggtagc gagatcacca ccggtccgga cggcccctcg gacacgtcgg
     8041 gaaaactcac gtcggtgcca cgcagttcga cgccttcgtc cttgccgacc accctgccct
     8101 tgatacggac gatggtgtcg gtggcgagtt gcagggccac ctgctggtag gccttgggga
     8161 acagcagcac ctcgacggag gcctccaggt cttcgacggt gacgatcgcc cacaggtcgc
     8221 cgttcttggt ctgcttgcgg ttgacgccgg tgatcatgcc ggcgatcgcg gtggtgatgt
     8281 cgcgcgggcc gtcctcggcg acgatggcac cgatcgaatg gtcgcggttg gccagcagca
     8341 catgctccag gcccagcaac gggtggtctg agacgtacag cccgagcatt tcgcgctcga
     8401 aggccagccg gacgcgcttg tcccactcgt cgatctcggg gatcggactg ctgaccaccg
     8461 cctcgccgcc ggcggggtcg ccgaacaggt cgtcctggcc gttggcctgg ttgcgcttga
     8521 ggtcgatgac ctcgtccacc ttctgctcga agcactccat cagcgagcgc cgggtgtggc
     8581 cgagtgagtc gaacgcgccg gccttgatca gcgattcgat cacccgcttg ttgcacacca
     8641 ccagcggcac gttcgacaga aactcgttga agtcacgggc cttgccgtgc tcgtcacggc
     8701 cttcgacgat gcccttgacc acgttgtcac cgacgttgcg gatggcgccg agcccgtagc
     8761 ggatgtcggt gccgaccggg gtgaacatgc cgtcggactc gttgacgtcg ggcgccagca
     8821 ccttgatgcc catgcgtcga cattcggcca ggtagatggc ggacttgtcc ttgtcgtcct
     8881 tgaccgactg caacagggcc gccatgtact cggccggata gttggccttg aggtaggccg
     8941 tccagtacga gatcaggccg tacgcggcgg tgtgcgcctt gttgaaggcg tagtccgaga
     9001 agggcaccag aatgtcccag agcgatttga tcgcgccggc cgagaagccg ttggcctgca
     9061 tgccggcctc gaagtgcacg aactcggcgt ccagcacctc cttcttcttc ttgcccatcg
     9121 cccggcgcaa ctggtcggcc gcaccgaggg tgtagttggc caccttcacc gcgatctgct
     9181 gcacctgttc ctggtacacg atcaggccgt aggtgaggtc gaggatctcg gccagcggct
     9241 cagccagctc ggggtggatc gccgtgatcg gctgcttgcc ggtcttgcgc agcgcgtagt
     9301 tggtgtggct gttggcgccc atcgggcccg gacggtacag cgccagcgcc gccgaaatat
     9361 cttcgaagtt gtcgggctgc atctgccgca gcagtacgcg catgccggtg ccgtccagct
     9421 gaaagatgcc gagggtgtcg ccggaggcca gcaactggta ggtcgccgga tcggtgggat
     9481 tcttgctgag ctcgtcgagg tcgaggtcga ggccgcggtt ggccttcacg ttggcgatcg
     9541 cgtcatcgag gatggtgagg ttgcgcagcc ccaggaagtc catcttgacc aggcccaacg
     9601 cctcgcagct cgggtagtcg aactgggtga tgatctggcc gtcggcctcg cgcttcatca
     9661 ccggaatgac gtccatcagg ggcgcggagc tcatgatcac gccggcggcg tgcacgcccc
     9721 actgccgctt gaggccctcg ataccccgtg cggtgtcgac cacctcctgc acctcggggt
     9781 cggactcgta cagggcccgg aactcgccac cctcggcgta gcgggcgtgc ttggggttga
     9841 acacctcggg cagcggaacg tccttgccca tcaccggcgg ggtgtaggcc ttggtgatct
     9901 tctcgcccag gccgaacggc ttgccgagca cccgtccggc gtccttgatc gcctgcttgg
     9961 ccttgatcgt gccgtaggtg acgatctggc agacgtggtc gtcgccgtac ttctcggtga
    10021 cgtagcggat gacctcgccg cggcggcgct cgtcgaagtc gacgtcgaag tcgggcatcg
    10081 agggccgctc ggggttgagg aagcgctcga agatcaggcc gtgcggcacc gggtcgaggt
    10141 cggtgatgcg cagggcgtag gcgcacatcg aaccggcgcc ggagcctcgt ccgggaccca
    10201 cccggatgcc gttgctcttg gcccagttga tgaagtcggc caccaccagg aaataccccg
    10261 aatacccctt gcccgcgatc accgtggttt cgaactcggc ctgttcggtg gcgtagctgg
    10321 gtacaccctc ggggaaacgc gcctcgagcc cgcggcgcac ctctttgacg aaccacgact
    10381 cctcggtctc gccggccggc accggaaact gcgccatgta ggtgccggtg ccctcgtcga
    10441 agtgggtgga gcagcgctcg gcgatggcca gcgtgttgtc gcaggcctcg ggcagctcgg
    10501 cccagatctg ccgcatctcg gcggggctct tcaggtagta gccgttgccg tcgaacttga
    10561 accggttggg ggtgtcacgg ttcgacccgg acgacacgca cagcagcgtg tcgtgagccg
    10621 cggcatcgtc cgcgtgcacg taatgcaggt cgttggtggc gaccaagggc agcttgaggt
    10681 ccttcgcgat gcgcagcaga tcgtggcgga cccgccgctc gatgtcgagg ccgtggtcca
    10741 tcaactcgca atagaagttg ccggccccga agatgtcgcg gaactcggcg gccgattcga
    10801 gcgcctggtc gtactggccg agacgcagat aggtctgcac ctcgccggag gggcagccgg
    10861 tggtggcgag caggcctttg ccgtagcggt gaagcagttc gcggtcggcg cgcggcttgt
    10921 agaaaaaacc ctccagcgaa ctcagcgagc tgagccggaa caggttgtgc atgccctcgg
    10981 agttctgcgc ccacatggtc atgtgggtgt aggcgccctt ggacgccacg tcgtcgccgg
    11041 tgccatcgcc gaactgcacg cgtttgcgct cgctgcggtg ggtcttcgga gtgaggtagg
    11101 cctccaggcc gatgatcggc ttcaccgcgc tgcccttggc caccttgtac cactcgtagg
    11161 cgccgaacag gttgccgtga tcggtgaccg ccagggcagg catgttcatc tgctcgcagc
    11221 ccttgaccag gtctttgagt cgcgccgcgc catcgagcat cgagtactcg gaatggcagt
    11281 gcagatgcac gaacccatcg gccatcggtt gcttcgacct ccatgcgtcg cggtgctgga
    11341 ccgggcagaa cgccaggggg aaggtccagg ggcagagacc caccctaacc ctccgcggcg
    11401 ggggttccgg acgacactcg ctgcgctggt tgccggctcg gcgtgtcgcg ggcgggctcg
    11461 gttcagtcgc cgaccaccgc cgcggtggcg gccgccacca gccgcaggtt gtgcggcagt
    11521 gcgccgtcga agccgggcgt gctcatggtg aggatcaccg tgtccagccg cgggttgttg
    11581 acgaacttgg tgctgaagaa cccctgccac gagtacgcgc cggggccgcc caaccagtgg
    11641 atcgagcccg ccggggcgtt ctccccctcc tgcaggtcga ccatgtaccc ccacttgttc
    11701 tgggtcagct gccagttggt caggtcgccg atctggttgg tcgtcatcaa ccgcactgag
    11761 gccgggctga gcacccgcac cccgtccagt tcgccctggt tgagcagcat ctgggcgaac
    11821 cggaagtagt cggcggtggt gccgtgcagg ccgcccgcgc cactgaacag cgtgcggtcg
    11881 tcgctgaagg tgtagttcgg gccgaggtga cccagcccca acggcgaggt gtcggtctgc
    11941 ttctcgtcgc ggcccgccca gtacaccgca gcgattcgat gccactggtc ctgtggcggg
    12001 aagaaccagg tctcgttcat gcgcagcggc tgaaagatgt gctgcctcag gtagctgtcg
    12061 aagtcctggc cggagaccac ttcgacgagc cggcacagca cgtccacgct gttgttggag
    12121 tagtcgaaca tcgtgcccgg atcggcgatc aggggcagcg aggcgatcag cctggtgttc
    12181 tcctccagcg tggtcggcgg cgtgcgcagg tcgtcgatca gcccggcctc ggcgtacagc
    12241 ttgggtacca cgtggtagat cgggggctcg aacaggccgt gccaccaggt gtttgtcatg
    12301 ccggcggtca tggtcagcag atcgtggatg gtgatcggac gggacgcggt gcgcagctgg
    12361 gtgaccgccc agtccttgac cacggcgacc ttggtgtcgg cgaactccgg caggtaggtg
    12421 gcgatcggtt cgtgcagcgc gatgcggccc tgttcgaaca gctgcagcac ggccaccgcg
    12481 ctgaccacct tcgacatcga cgccagccgg aagatcgagt cggtggccat ctcgacgtcg
    12541 tcgtccgcct tgccgaacgc cttgaagtag gccaccttgc agtgccgggc gaccaggacc
    12601 accgagccct gataggcgtt ctcggccagg taccgctgct gcagggcgtc gatgttggcc
    12661 aggtattggc tggagacgcc aacctcttcg ggatcgacag gggtgtacag ctcgctgctc
    12721 atgggcgtcc tttcgcgacg gtgcggaccc cgatcaggcc ggtcaacggt ggacgaccgg
    12781 ggcactggga tgtttcgact ctagtgcgtg ccggacctgt atggacaggt ccagaccagt
    12841 tgggtgaggg cggggtcgag ccgcggcggg ctccaatggc agacgcagtt atgcgttctt
    12901 ctgttcggag tcccaccagc gcagcacccg ggtggcgtag aaagtgagcc acgggg
//
LOCUS       DAOSUG010000015        12877 bp    DNA     linear   ENV 10-JUN-2024
DEFINITION  MAG TPA_asm: Micropruina sp. isolate AMR_MDS_2195
            NODE_2867_length_12877_cov_5.033380, whole genome shotgun sequence.
ACCESSION   DAOSUG010000015 DAOSUG010000000
VERSION     DAOSUG010000015.1
DBLINK      BioProject: PRJNA925477
            BioSample: SAMN32864262
            Sequence Read Archive: SRR4236673
KEYWORDS    WGS; ENV; Metagenome Assembled Genome; MAG; Third Party Data; TPA;
            TPA:assembly.
SOURCE      Micropruina sp. (wastewater metagenome)
  ORGANISM  Micropruina sp.
            Bacteria; Bacillati; Actinomycetota; Actinomycetes;
            Propionibacteriales; Nocardioidaceae; Micropruina.
REFERENCE   1  (bases 1 to 12877)
  AUTHORS   Abdulkadir,N., Saraiva,J.P., Zhang,J., Stolte,S., Gillor,O.,
            Harms,H. and Rocha,U.
  TITLE     Genome-centric analyses of 165 metagenomes show that mobile genetic
            elements are crucial for the transmission of antimicrobial
            resistance genes to pathogens in activated sludge and wastewater
  JOURNAL   Microbiol Spectr 12 (3), e0291823 (2024)
   PUBMED   38289113
REFERENCE   2  (bases 1 to 12877)
  AUTHORS   Abdulkadir,N.
  TITLE     Direct Submission
  JOURNAL   Submitted (24-MAR-2023) Environmental Microbiology, Helmholtz
            Center for Environmental Research -UFZ, Permoserstr 15, Leipzig
            04318, Germany
COMMENT     The annotation was added by the NCBI Prokaryotic Genome Annotation
            Pipeline (PGAP). Information about PGAP can be found here:
            https://www.ncbi.nlm.nih.gov/genome/annotation_prok/
            
            ##Genome-Assembly-Data-START##
            Assembly Method        :: SPAdes v. 3.11.1
            Genome Representation  :: Full
            Expected Final Version :: Yes
            Genome Coverage        :: Not Applicable
            Sequencing Technology  :: Illumina MiSeq
            ##Genome-Assembly-Data-END##
            
            ##Genome-Annotation-Data-START##
            Annotation Provider               :: NCBI
            Annotation Date                   :: 05/04/2023 04:57:10
            Annotation Pipeline               :: NCBI Prokaryotic Genome
                                                 Annotation Pipeline (PGAP)
            Annotation Method                 :: Best-placed reference protein
                                                 set; GeneMarkS-2+
            Annotation Software revision      :: 6.5
            Features Annotated                :: Gene; CDS; rRNA; tRNA; ncRNA
            Genes (total)                     :: 2,776
            CDSs (total)                      :: 2,750
            Genes (coding)                    :: 2,714
            CDSs (with protein)               :: 2,714
            Genes (RNA)                       :: 26
            tRNAs                             :: 24
            ncRNAs                            :: 2
            Pseudo Genes (total)              :: 36
            CDSs (without protein)            :: 36
            Pseudo Genes (ambiguous residues) :: 0 of 36
            Pseudo Genes (frameshifted)       :: 2 of 36
            Pseudo Genes (incomplete)         :: 32 of 36
            Pseudo Genes (internal stop)      :: 2 of 36
            ##Genome-Annotation-Data-END##
FEATURES             Location/Qualifiers
     source          1..12877
                     /organism="Micropruina sp."
                     /mol_type="genomic DNA"
                     /submitter_seqid="NODE_2867_length_12877_cov_5.033380"
                     /isolate="AMR_MDS_2195"
                     /isolation_source="Activated sludge of municipal
                     wastewater treatment plants"
                     /db_xref="taxon:2737536"
                     /environmental_sample
                     /geo_loc_name="Canada"
                     /lat_lon="49.25 N 123.1 W"
                     /collection_date="2015-06-16"
                     /metagenome_source="wastewater metagenome"
                     /note="metagenomic"
     gene            321..1642
                     /locus_tag="PLF56_01000"
     CDS             join(321..615,615..1642)
                     /locus_tag="PLF56_01000"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_076611812.1"
                     /ribosomal_slippage
                     /note="programmed frameshift; Derived by automated
                     computational analysis using gene prediction method:
                     Protein Homology.
                     GO_function: GO:0004803 - transposase activity [Evidence
                     IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="IS3 family transposase"
                     /protein_id="HOQ52170.1"
                     /translation="MAAPRKYSVELQQRATRMALDARKDPESSRGAIKRIADQLGVHP
                     EALRNWVRQAEIDDGVRPGVSSEDTARIVELERENRELRRANEILKTSAAFFAAGGAR
                     PQDQVGRQVPVAVVVQSIDAHRDRIVEGRPLGVEPICEVLTESGVRIAPSTYYAARNR
                     PLSARAVRDAELVEDIKTAHKANLGVYGARKIHAVLNREGISVARCTVERLMRAEGLR
                     GIPREKTRRTTFGEGAETERPEDLVKRKFIATGPNQLWVADLTYVRTHAGWTYVAFVL
                     DVFSRMVVGWQVSTSLRTDLALDALDMGLWARGRAGQDVTGLIHHSDRGGQYRAIRYT
                     ERLAEAEAVASVGSKGDSYDNAMAEALNSLFKAECIRNPAMRPIGGWKSVADVEIAVA
                     EYVDWFNHRRLHGELGLVPPAEYEATYWASQPSQHYRKNPVLTEAGTN"
     gene            1972..2139
                     /locus_tag="PLF56_01005"
     CDS             1972..2139
                     /locus_tag="PLF56_01005"
                     /inference="COORDINATES: ab initio
                     prediction:GeneMarkS-2+"
                     /note="Derived by automated computational analysis using
                     gene prediction method: GeneMarkS-2+."
                     /codon_start=1
                     /transl_table=11
                     /product="hypothetical protein"
                     /protein_id="HOQ52171.1"
                     /translation="MKLLETEQISYERPGAGRHRRVRLSDVHRLTLDEAIESAYELVV
                     GNPRRAFKRQR"
     gene            complement(2162..3394)
                     /locus_tag="PLF56_01010"
     CDS             complement(2162..3394)
                     /locus_tag="PLF56_01010"
                     /inference="COORDINATES: protein motif:HMM:NF012356.2"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_function: GO:0003824 - catalytic activity [Evidence
                     IEA];
                     GO_process: GO:0005975 - carbohydrate metabolic process
                     [Evidence IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="alpha-amylase family glycosyl hydrolase"
                     /protein_id="HOQ52172.1"
                     /translation="MSWSNDMIWWHCYPLGFVQAEHDAIDHVEHRLWRLANWLDYLIE
                     LGANGLLLAPIFASLTHGYDTIDHWRIDSRLGDEQDFDDLVRQAKDRGVRLLLDGVFN
                     HVSSEHEIVRRALAGGPTSSDGDWIRWVDQYPRGFEGNLNMVELNLANPAVQTYVVRA
                     MNHWLERGADGWRLDAAYAAGAEAWRPIVQQVKQAHPDAWILAEVIHGDYADFVARSG
                     VDSVTQYELWKAIWSSLNDTNFHELAWTLQRHAEFSTTFRPFNFIGNHDTTRIATKLT
                     RAQDLPLAHALLMLLPGVPAIYAGDEQGFTGEKTDGSTGDDAVRPPFPVDPSGLAGFG
                     RPIFELDQRLIHLRRTHPWLVTAAVTVSHVGADTIAIDLRGDGHALTLALNARPEPVE
                     VSGVNGGLHVGAHGWALG"
     gene            complement(3523..4083)
                     /locus_tag="PLF56_01015"
     CDS             complement(3523..4083)
                     /locus_tag="PLF56_01015"
                     /inference="COORDINATES: protein motif:HMM:NF015674.2"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="DUF308 domain-containing protein"
                     /protein_id="HOQ52173.1"
                     /translation="MIDLMKRSSTALIVFGVLATIFGLIAAFFPVATALTLVLLWGIY
                     ALVDGVTAAVLAFSGDARQSRGFLIFAAVVGIAAGLFAILQPVKSAVALAWVIGLWLL
                     VRGVLELVGAFTNTDVQSGWLLALGGLLWMLGGILVISFPGVAALAISLWLGLLAIVW
                     GIVLVWAGLHMRTTAKLSEPIPGEVV"
     gene            4307..5629
                     /locus_tag="PLF56_01020"
     CDS             4307..5629
                     /locus_tag="PLF56_01020"
                     /inference="COORDINATES: protein motif:HMM:NF025025.2"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="glycosyltransferase family 2 protein"
                     /protein_id="HOQ52174.1"
                     /translation="MPWLDVIAWTLQTIMVIGAIYGLYYVAVGLNAFGELALPDPQPG
                     DKQHRYAILVCAKNESTVIDQLLVTIGNLNYPRELYDVFVVADNCTDDTAEICRKAGA
                     YVYERTDATRKGKGFAMAWFFERFSVDHAGQYDVCVVFDADNVVEVGFLEALNTRFNA
                     GETIVVGYRTGKNPSSSWVAGCSTLYWLLQTRFFHLPRNRFGWSLFSVSGTGFGFKLS
                     ILDDKGWNTRTQCEDIEFTLNAIADGHRAVLEPRAVFFDEQPQGLWTSIKQRYRWALG
                     GVQIVPICTPKLWRRVRSGDRLATDGLIYSVGLLISAISGWCGMAFSLLLALRTGRWD
                     LLVLGALVFGAFAYVFTGIFGWITVLLEDARWPGMWKTVLLFPLHLGIWWVLMLVALV
                     YRDNTWHDIPHTVRLSLSEIEAEHHTGRLMPLNRPRRERPDPGPSQQE"
     gene            complement(5756..6370)
                     /locus_tag="PLF56_01025"
     CDS             complement(5756..6370)
                     /locus_tag="PLF56_01025"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_015070090.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="NUDIX hydrolase"
                     /protein_id="HOQ52175.1"
                     /translation="MQNRASLVSVDVITLRYNPQLRRVEMATKPRTTEPFVGKPALPG
                     VLLHEGERLAAAAHRAVTTKVGFDVSALGQLTVFDQPNRDPRGYSLSVAMWAVGDGDA
                     DWYSFDDVPNLAFDHNVMLFECRPLLVDMLWRDLEFTRALTGPVFPVSAAVGITQTLS
                     GITPDRGNLNRRLAALRGLTVSSRRVVLGRGRPGTVWEWDQPAA"
     gene            6804..7664
                     /locus_tag="PLF56_01030"
     CDS             6804..7664
                     /locus_tag="PLF56_01030"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_011756859.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_component: GO:0016020 - membrane [Evidence IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="EamA family transporter"
                     /protein_id="HOQ52176.1"
                     /translation="MRTAWARIDPVWMVLVGIASVQLGAAFAKDLFATTTAVATAWLR
                     LCFSALIFLTFARPRWRARAPREWVVVGCYGLALAGMNWAIYESFARIPIGVAVTIEF
                     LGPLAVAVAGSRRARDLIWVGLAAVGVALLGLQPIEADWLGLGLALVAGALWAAYILL
                     AGPTGRHFEGVSGVTVASMMGAVLLAVPALTLGSPVFNGHVLGVALAVGLLSSVIPYG
                     LEMIALRRMRAGLFGILMSLEPAAAALFALLVLGERLTLVECLAMACVVAASVGATRS
                     RTTSESPLID"
     gene            7754..8626
                     /locus_tag="PLF56_01035"
     CDS             7754..8626
                     /locus_tag="PLF56_01035"
                     /inference="COORDINATES: protein motif:HMM:NF024950.2"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="SDR family oxidoreductase"
                     /protein_id="HOQ52177.1"
                     /translation="MSDTLPLAGQTALVTGVSRRRGIGFAIAGRLAELGASVFAQHHR
                     PHDLELPWGGDDLDALRNELRTLLSASARFGDLAADLSDPAGIPMLIERATALTGSLD
                     ILVCNHAKSGDDGSILDMTAERLDAFWQVNTRSTLLLTAEFARTRTGAPSEGARRPGE
                     RVSGLKPYARPTGRVFWMTSGQQLGPMRGEVAYAASKAALAGVTATVAAELLELGIVL
                     NTINPGPVNTGYLDAETTDRPLESMQQYLASTPFGRVGRPQDPAHLIGWLATDEGAWV
                     VGQVLTSDGGFSLA"
     gene            complement(8850..9716)
                     /locus_tag="PLF56_01040"
     CDS             complement(8850..9716)
                     /locus_tag="PLF56_01040"
                     /inference="COORDINATES: protein motif:HMM:NF013117.2"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_function: GO:0008381 - mechanosensitive monoatomic ion
                     channel activity [Evidence IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="mechanosensitive ion channel family protein"
                     /protein_id="HOQ52178.1"
                     /translation="MTPMAIEWIWPDTPLAIVITLVVAITGRWLLLRAIRRGSDLAVG
                     RANQRRNDQGNHDAALSAARHAARAATMGSLLRSVANAGIAIVTVLTVMAILNIPLTP
                     ILASAGVGGVALGFGAQSLVKDYLSGIFMIVEDQYGVGDLVDLGEASGTIEDVGLRVT
                     RLRDGSGQVWYIRNGEITRVGNQSQGWSTATVDIPVDSSEDSATVIQILSDVCSEVAA
                     DPQWQGVLLEEPSVAGVQSVEAGRMTIRITAKTEPNQHWGVQRAILDRELQALKDAGI
                     KGPRILPAPPTL"
     gene            complement(9856..10563)
                     /locus_tag="PLF56_01045"
     CDS             complement(9856..10563)
                     /locus_tag="PLF56_01045"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_013864960.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_function: GO:0005524 - ATP binding [Evidence IEA];
                     GO_function: GO:0016887 - ATP hydrolysis activity
                     [Evidence IEA];
                     GO_function: GO:0042626 - ATPase-coupled transmembrane
                     transporter activity [Evidence IEA];
                     GO_function: GO:0140359 - ABC-type transporter activity
                     [Evidence IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="ABC transporter ATP-binding protein"
                     /protein_id="HOQ52179.1"
                     /translation="MLEIKDLVVAYGKVRAVKGISFTVEQGQVVSLLGTNGAGKTTTL
                     RTISGLQRAESGEIWFNGERIDKTPAHQVVTRGLAHSPEGRRIFPRLTVEDNLLLGAF
                     ARKDADGIAKDLTRVYDLFEILHERRTQPAGTFSGGEQQMLAIGRAMMSRPKLLMLDE
                     PSMGLSPLMMQRIMSTIRALQSEGVTILLVEQNAAAALKLSDRAYVLEVGQITLEGTG
                     EQLLGDDRVKRAYLGED"
     gene            complement(10556..11389)
                     /locus_tag="PLF56_01050"
     CDS             complement(10556..11389)
                     /locus_tag="PLF56_01050"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_020388727.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_function: GO:0005524 - ATP binding [Evidence IEA];
                     GO_function: GO:0016887 - ATP hydrolysis activity
                     [Evidence IEA];
                     GO_function: GO:0042626 - ATPase-coupled transmembrane
                     transporter activity [Evidence IEA];
                     GO_function: GO:0140359 - ABC-type transporter activity
                     [Evidence IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="ABC transporter ATP-binding protein"
                     /protein_id="HOQ52180.1"
                     /translation="MSTDAVSVAAPEHRAGREEILAARNVTMRFGGLVAVDNVDFSVH
                     TGEIVGLIGPNGAGKTTFFNCLTGMYKPTSGEVRFAGEVLPPKPRKVVVAGMARTFQN
                     IRLFPNMTALENVMVGRYCRTSAMALTSIVRGPKFRREEQETRDRASELLEYVGMKGQ
                     GAHLARNLPYGDQRRLEIARALATDPKLLLLDEPTAGMNPQETRQAEELIFKIRDSGL
                     AIVVIEHDMRFIFNLCDRVLCLVRGKTLIEGTAAEVQSDPRVIEAYIGSPETEDEAGN
                     A"
     gene            complement(11386..>12877)
                     /locus_tag="PLF56_01055"
     CDS             complement(11386..>12877)
                     /locus_tag="PLF56_01055"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_013864962.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=2
                     /transl_table=11
                     /product="branched-chain amino acid ABC transporter
                     permease"
                     /protein_id="HOQ52181.1"
                     /translation="RGFAPAVLKVSKRVGWTRGTKAAGIGVLAYIGFILLAIALELGG
                     LVTVEWGGWVALIGALLGFTGSKLLTPGRTPGLERLRSAAWVEIVAIALLLLFALLLT
                     AYALNQTDGGTFLAIIVFVATITAVIMRSGLGGWLSRAGQKHRTVLVFSSFTVAFLFP
                     FTQNGSDANMSIATQVLLFAATAVGLNIVVGLAGLLDLGYIAFLGAGAFTAATLSESA
                     FATIDWHPPFIVVMLIAGLISASLGLLIGSPTLRVSGDYLAIVTLAFGEIFRITMNNL
                     DGEDGPDLVHGSSGIPAIPNLNLFGFDFGEVHTVLGIPLGRFSNYFFLMLLLMGLIVL
                     IFTRLNHSRIGRGWVAIREDEKAAEAMGVNVFGLKLLAFAGGAFLAGLAGSVKAHVDQ
                     SVTPDQYIFLESAFLLAAVVLGGMGTVMGVILGATLLKLVPEKLRFVNEYRMLLFGLL
                     MVVMMRVRPEGMIPNQRRALEFHSDDEELVEEVEEDLPYEFAEEKS"
BASE COUNT         2162 a         4260 c         4397 g         2058 t
ORIGIN      
        1 gacgttgatg atggtgcggc gcagggtggc ccagcgtgcc ttggccagcc cgtcagtcct
       61 cggacttgca aagcgagcgg cggatgatca atgcggagac acatacggga cacggggtac
      121 tcaatgttgt tgacaaaagg aagcaggcgt tgggaagaca ccaggcctcc gactcggctt
      181 ccggctaggc taatcgaccg tcgccaggct caacctttcg catcgtcggg agcaccctat
      241 tcgctctgat aaggcggagg tcagtgaatc gccccgggtc tgatggaggc tctcaatcct
      301 gggaagggat gatgagagtc atggcagcac cgaggaagta cagcgtggag ctccagcagc
      361 gggcgacgcg gatggcgttg gacgccagga aggatccgga gtcgtccagg ggcgcgatca
      421 agcggatcgc ggaccagttg ggggtgcatc cggaggcgtt gcggaactgg gtacgccagg
      481 ccgagatcga cgatggggtc aggcctgggg tgtcgagtga ggacacggcc cggatcgtgg
      541 agttggagcg ggagaaccgt gagttgcggc gggcgaacga gatcttgaag acctctgcag
      601 cttttttcgc ggccgcggag ctcgaccgca ggatcaagta ggccgccagg ttccggtcgc
      661 ggtggtcgtg cagtccatcg acgcccaccg tgaccggatc gtggagggcc gcccgctcgg
      721 ggtcgagccg atctgtgagg tcctgaccga gtcaggagtc cggatcgccc cgagcaccta
      781 ctacgccgcc aggaaccggc cgctctcagc cagggcggtg cgggacgccg agttggtgga
      841 ggacatcaag accgcccaca aagcgaacct gggtgtctat ggggccagga agatccatgc
      901 cgtgctgaac cgcgagggca tcagcgtggc ccgttgtacg gtcgagcggc tgatgagggc
      961 tgagggcctg cgtgggatac cacgagagaa gacccgccgg accaccttcg gcgagggcgc
     1021 cgagaccgag cggcccgagg acctggtgaa gcgcaagttc atcgcgaccg gacccaacca
     1081 gctgtgggtg gccgatctaa cctacgtccg cacccatgcc ggctggacct acgtcgcgtt
     1141 cgtcctggat gtgttctccc ggatggtcgt gggctggcag gtgtccacca gcctgcgcac
     1201 cgacctggcc ctggacgccc tcgacatggg cttgtgggcc cgcggccgcg ccggccagga
     1261 cgtcaccggc ctgatccacc acagcgaccg aggaggtcag tatcgagcga tccgctacac
     1321 cgaacggctc gccgaagccg aggccgtcgc ctcggtcgga tccaaaggcg acagctacga
     1381 caacgcgatg gccgaagcgc tgaactcgct gttcaaagcc gaatgcatcc gcaaccccgc
     1441 gatgcgaccg atcggcggct ggaagagcgt cgccgacgtc gagatcgcgg tcgccgagta
     1501 cgtcgactgg ttcaaccacc gacgcctcca cggcgaactc gggctcgtcc cacccgcaga
     1561 gtacgaggcc acctactggg catcccagcc cagccagcac taccgtaaga accccgtcct
     1621 caccgaggcc ggaaccaact aaccgagcct ccacgaaacc cggggcgatt cacagtggtt
     1681 caagtccact taggcccact ccacgaaaag ccgctctcac aaggtcgaac atctgaccag
     1741 agggcggctt tcgcgtgcga gtgcccctac cgggcgccaa ggccgcggtc tcgttgccac
     1801 actctggctg cggggcgttc cgctcgcgtc ggggtgtgga ggcgcaaccg gccggggcag
     1861 cacagcgcgc accttagtcg ccggtggcat tgctcgggga cctgcggggc gggcgtcgtg
     1921 gtcgctgtgt ccgacaggag catgcgaccg tggcatcagc cgcccgattt gatgaagctc
     1981 ctcgagaccg agcagatctc ctacgagcgt ccgggggccg gccgacatcg tcgggtgcgg
     2041 ctcagtgacg ttcaccgact gaccctcgac gaggccatcg agagcgccta cgaactcgtc
     2101 gtcggcaacc cgcgccgggc attcaagcgg cagcggtagc cgccgcggcg ggcgaggttg
     2161 ctcagcccag ggcccagccg tgggcaccca cgtgcagccc gccgttcacc ccgctcacct
     2221 cgaccggctc gggtcgcgcg ttcagggcga gggtcagggc atgcccgtcg ccgcgcaggt
     2281 cgatggcgat cgtgtcggcg ccgacgtgcg agacggtcac ggctgcggtg accaaccacg
     2341 gatgcgtccg gcgcaggtgg atcagccgct gatcgagttc gaagatgggc cggccgaaac
     2401 ccgccagccc cgagggatcg accgggaacg gcggacggac ggcgtcatcg ccggtggagc
     2461 cgtcggtctt ctcaccggtg aagccctgct cgtcgccggc gtagatggcc ggtacgcccg
     2521 gtagcagcat gagcagcgcg tgcgccagcg gcaggtcctg cgcgcgggtg agtttggtgg
     2581 cgatgcgcgt ggtgtcgtga ttgccgatga agttgaacgg acggaacgtt gtcgagaact
     2641 cggcgtgccg ctgcaacgtc caggccagct cgtgaaagtt ggtgtcgttg agcgaactcc
     2701 agatcgcctt ccacaactcg tactgggtga ccgaatcaac gcccgagcgg gcgacgaaat
     2761 cggcgtagtc gccgtgaatc acctcggcca ggatccaggc gtcggggtgg gcctgcttga
     2821 cctgctgcac gatcgggcgc cacgcctcgg ccccggccgc ataggcggcg tcgagccgcc
     2881 acccgtccgc accgcgctcg agccagtggt tcatcgcccg caccacgtag gtctgcaccg
     2941 cagggttcgc caggttcaac tcgaccatgt tgaggttgcc ctcgaagccg cgcgggtact
     3001 gatccaccca gcggatccag tcaccgtcgc tggacgtcgg gccaccggcc agggcgcgcc
     3061 ggacgatctc gtgctcggac gacacgtggt tgaagacccc gtccagcagc agcctgacgc
     3121 cgcgatcctt ggcctggcgg acgaggtcgt cgaagtcctg ttcgtcaccc aaccgggaat
     3181 cgatgcgcca gtggtcgatg gtgtcgtagc cgtgggtgag ggaggcgaag atcggcgcga
     3241 gcagcaggcc gttggctccc agctcgatca ggtagtccag ccagttggcc aggcgccaga
     3301 ggcggtgctc gacgtggtcg atcgcgtcat gctcggcctg aacgaatccc agcgggtagc
     3361 agtgccacca gatcatgtcg ttcgaccagg acactgtgcc tcctgcagtt ggggcccgat
     3421 tcacccaccc taacggccac ggcgcgtgga cgtgctgacc agcctggtgc ggggccctcg
     3481 gcgagacccg gctggactcc cgcgagaccc ggctagaccc ggctagacca cttcgccggg
     3541 gatcggctcg gacagcttgg ccgtcgtacg catgtgcaac cctgcccaga ccagcacgat
     3601 gccccagacg atggccagca gccctagcca caggctgatc gcgagcgccg ccacgcccgg
     3661 aaagctgatc accagaatgc cgccgagcat ccacaacaga ccgcccaggg cgagcagcca
     3721 gcccgactgc acgtcggtgt tggtgaaggc cccgaccaac tcgagaacac cacggacgag
     3781 cagccacagg ccgatcaccc aggccagcgc cacggcgctc ttgaccggtt gcagaatggc
     3841 gaacaggccg gccgcgatgc cgaccaccgc cgcgaagatc agaaagccgc gcgattgccg
     3901 agcgtcgccg ctgaaagcga gcacagcggc ggtgacgccg tcgaccaggg catagatgcc
     3961 ccagagcagg accagcgtca aagcggtggc gaccggaaag aatgcggcga tcagcccgaa
     4021 gatcgtggcg agcactccga agacgatcag cgcggtcgag gatcgcttca tcaggtcgat
     4081 catggtgctc tccctcatcg tcggcgggcg ggccagccgg tccccacttt ctcacggacc
     4141 acagcccgct cacagcctcg cgacgccggc gccggtgaga gagtcccaaa cgttggctga
     4201 gtgggcgacc cgtcgccctc gtcccgagtt gcggggcggg cgcgctctag actgtcagtt
     4261 ctcgcccgga cgaccggcgc gtgaccccgc tgcaaggagc gtgacggtgc cctggctcga
     4321 cgtgatcgcc tggacgctcc agacgatcat ggtgatcggg gccatctacg gtctgtacta
     4381 cgtcgcggtc gggctgaacg ctttcggcga actcgcgctg cccgacccgc aaccgggcga
     4441 caaacagcat cgctacgcga tcctggtgtg cgccaagaac gaatccacgg tgatcgacca
     4501 gttgctggtg acgatcggca acctgaacta tccccgcgag ctctacgacg tgttcgtagt
     4561 ggccgacaac tgcaccgacg acactgccga gatctgccgt aaggccggcg cgtacgtcta
     4621 cgaacgcacc gacgccacgc gcaagggcaa gggcttcgcg atggcgtggt tcttcgagcg
     4681 gttcagcgtc gatcacgccg gtcagtacga cgtctgcgtg gtcttcgacg ccgacaacgt
     4741 ggtcgaggtc ggctttctgg aggcgctcaa caccaggttc aacgccggcg agaccatcgt
     4801 ggtcgggtac cgcacgggca agaacccctc ctcgtcgtgg gtggccggct gcagcaccct
     4861 gtactggctg ctgcagaccc gcttctttca cctgccccgt aaccggttcg gctggtccct
     4921 gttctcggtc agcggcacgg gtttcggctt caagctgtcg atcctggacg acaagggctg
     4981 gaacacccgc acccagtgcg aagacatcga gttcaccctg aacgcgatcg cggacggtca
     5041 tcgggccgtt ctcgagcccc gtgcggtgtt cttcgacgag cagccccagg gtctgtggac
     5101 ctcgatcaag cagcgctacc ggtgggcgct gggcggcgtc cagatcgtgc cgatctgcac
     5161 cccgaagctc tggcgccggg tgcgttccgg cgaccgtttg gccaccgacg ggctgatcta
     5221 cagcgtgggt ctgctgatct cggccatctc gggttggtgc ggcatggcgt tcagcctgtt
     5281 gctcgcccta cgcaccggac gctgggatct gctggtgctg ggcgccctgg tcttcggcgc
     5341 tttcgcctac gtgttcaccg gcatcttcgg gtggatcacg gtgctgctcg aggacgcccg
     5401 gtggccgggc atgtggaaga cggtgctgct gtttccgttg cacctgggca tctggtgggt
     5461 gctgatgctg gtggccctgg tctaccgcga caacacctgg cacgacatac cgcacaccgt
     5521 gcgactcagc ctcagtgaga tcgaggccga acaccacacc ggccggctga tgccgctcaa
     5581 ccggccccgg cgcgagcgcc ccgacccggg accgtcccaa caggaatgag cccgcgccac
     5641 ccacgcatgc gctgatcacc gcacggttcg cgcatcgatc agcacctcct cgaggggtac
     5701 ggaaacgatg atccagcgca atagcccggc agccggctca ccgaccgttg cctgctcagg
     5761 cggccggttg gtcccactcc cacacggtgc ccgggcggcc gcggcccaac accacccgcc
     5821 ggctgctcac cgtgagccca cgcagggcgg cgaggcgacg gttgaggttg ccgcggtcgg
     5881 gggtgatgcc actgagggtc tgcgtgatgc cgacggcggc cgagaccgga aacacgggcc
     5941 cggtcagagc ccgggtgaac tccaggtcgc gccacagcat gtcgaccagc agcggacggc
     6001 actcgaacag catgacgttg tggtcgaagg caagattggg cacatcatcg aaggagtacc
     6061 agtcggcgtc gccgtcaccg acagcccaca tggcgaccga cagcgagtag ccacgcggat
     6121 cgcggttggg ttgatcgaaa acggtgagct ggccgagcgc ggagacgtcg aaaccgacct
     6181 tcgtggtcac tgctcgatgc gcggcggcgg ccagccgctc accctcgtgc aggagcacgc
     6241 ccggcagtgc cggcttgccg acgaacggtt ctgtggtgcg aggtttggtg gccatttcga
     6301 cccgccgcaa ctgggggttg taccgcaggg tgatcacgtc gacggaaacc agcgatgccc
     6361 ggttctgcat ctgtctgctc cctccacgtc gagacctctc attagtctat tggactataa
     6421 cgtgccgaca agggttcttg ccggatcgga caaacagagg gccggcccag ctcgtcagag
     6481 gtcccatcga ctccccccga acaacgagcg gctgtacgtg ctcgggattc tgaaatgtcg
     6541 accccagtcg ggtcgtcgac ccgcgacacg ccgtggtcca tgccttctct gggggaggat
     6601 ttcagaatcg agccccgaag gccgtgttcg caggttgggg cgtggtgccc gccgcctctc
     6661 cgggcagtac ccacgcggat tctgaaatcc tcccccaggg tcactgcaac tgtcggcgtg
     6721 tcgcagctcg ccggttcggc tggagtcgac atttcagaat ccaccagacg tgagagtgcg
     6781 tcgtgctgtg ggatagtcgc ccagtgcgaa cggcctgggc ccggatcgat ccggtgtgga
     6841 tggtgctggt cgggatcgcc tcggtgcagt tgggcgcggc attcgccaag gatctgttcg
     6901 ccaccaccac ggcggtggcc accgcctggc tgcggttgtg cttctcggcc ctcatctttc
     6961 tgaccttcgc ccgcccacgc tggcgagccc gagcgcctcg cgagtgggtg gtggtcggct
     7021 gttacggcct ggcgcttgcc ggaatgaact gggcgatcta cgagagcttc gcccgcattc
     7081 cgatcggcgt ggcggtcacc atcgagtttc tgggaccgct ggcggtcgcg gtggccggct
     7141 cgcgccgggc gcgtgacctg atctgggtcg ggctggccgc cgtgggcgtg gcgctgctcg
     7201 gtctgcaacc gatcgaggcg gactggctgg ggctagggct ggcgctggtg gccggtgccc
     7261 tctgggccgc ctacattctg ctggccggcc cgaccgggcg gcacttcgag ggcgtctcgg
     7321 gcgtcaccgt cgcgagcatg atgggcgcgg tgctgttggc ggtgccggcg ctgaccctcg
     7381 gatcgccggt gttcaacggg cacgtgttgg gtgtcgcgtt agcggtcgga ctgttgtcgt
     7441 ccgtcattcc ctacggcttg gagatgatcg ccctgcggcg gatgcgtgcg gggctgttcg
     7501 gcattctgat gagcctggag ccggcggcag cggcgctgtt cgcgctgctg gtgctcggtg
     7561 agcgcctcac cctcgtcgaa tgcctggcga tggcctgcgt ggtggcggcc agcgtcggcg
     7621 cgacccggtc acgcacgacg tccgaatctc cgttgatcga ctgaggcccg agcgcaagac
     7681 tgatctgtgg gcggatggac cagtaccgtt cgaggccacc accgccgacg ccccgtcacc
     7741 gaaaggcgca gccatgtccg acacccttcc gctggccggc cagacagccc tggtcaccgg
     7801 ggtttcccgc aggcggggaa tcggcttcgc catcgccggc cgactggccg agttgggcgc
     7861 cagtgtcttc gcgcagcacc accgtccgca cgatctcgag ctgccgtggg gcggcgacga
     7921 cctggacgcc ctgcgaaacg agttgcggac gctcctgtcg gcttcggcgc ggttcggcga
     7981 cctggccgcc gacctcagcg acccggccgg catcccgatg ctgatcgagc gggcgacggc
     8041 actgaccggc agcctcgaca ttctggtctg caaccatgcc aagagcggcg acgacggcag
     8101 catcctcgac atgaccgccg agcgcctcga tgcgttctgg caggtcaaca cccgctcgac
     8161 gctgctgctc accgcggagt tcgcgcgcac caggacggga gcgcccagcg agggcgcccg
     8221 gcgtccgggt gaacgggtga gcggcttgaa gccctacgcg cgaccgaccg gacgggtgtt
     8281 ctggatgacc tcgggccagc agctggggcc gatgcgcggc gaggtcgcct acgcggccag
     8341 caaggcggcg ctggccgggg tgaccgcgac cgtggcggcg gagttgctgg aactcggcat
     8401 cgtgctgaac accatcaacc ccggaccggt caacaccggg tacctggacg ccgagaccac
     8461 cgaccgcccc ctcgaatcga tgcagcagta tctcgcctcg acgccgttcg gacgggtggg
     8521 gcggccgcag gacccggccc acctgatcgg ctggcttgcc acggacgaag gtgcctgggt
     8581 cgtgggccaa gtgctgacca gcgacggcgg tttctcgttg gcctagggcg tgtcgcataa
     8641 ccgtgggcgt tccgcgagga cgtgcccagt gcgtctgatc gcaaggcgga agacgagtcg
     8701 atagtgggtc ctctcgccga ggatgacaac gcagcgagcg ggcgtactgg gtgggccgga
     8761 gcgtggtcag ggttatgcga cacgccctag ctcgccctct agctccctct agctcgggtc
     8821 tcgaccagct cgaccagcga ggggtgacgt cacagcgtgg gtggtgccgg caggatgcgc
     8881 ggccctttga tgcccgcgtc cttcagtgcc tgcagctcac gatcgaggat ggcgcgctgc
     8941 accccccagt gctggttggg ctcggtcttg gcggtgatcc ggatcgtcat gcgacccgct
     9001 tccaccgact ggacgcccgc cacactcggt tcttccagca gcacgccctg ccattgcggg
     9061 tcggcggcga cctcgctgca cacatcgctc agaatctgga tcaccgtggc cgagtcctcg
     9121 ctcgaatcca ccggaatgtc gacggtggcg gtcgaccagc cctggctctg gttgccgacc
     9181 cgggtgatct cgccgttgcg gatgtaccag acctgccccg agccgtcccg cagccgggtg
     9241 actcgcagtc cgacgtcctc gatggtgccg gaggcttcgc ccagatcgac caggtcgccc
     9301 accccgtact gatcctcgac gatcatgaag atgccggaca ggtagtcctt gaccaggctc
     9361 tgggcgccga aaccgagcgc cacgccgccg acgcccgcgg acgccagtat cggcgtcaac
     9421 ggaatgttga ggatcgccat cacggtgagg acggtcacga tggcgatgcc ggcgttcgcg
     9481 acgctgcgca gcagcgagcc catggtggcc gcccgcgccg cgtgacgtgc cgcagagagg
     9541 gcggcatcgt gattgccctg gtcgttgcgt cgctggttgg cccggccgac ggcgaggtcg
     9601 gagccgcgtc tgatcgcgcg cagcagcagc caccgtccgg tgatcgcgac caccagggtg
     9661 atcacgatcg ccagcggtgt gtcgggccag atccactcga tggccatcgg cgtcatgggg
     9721 tccattctca tgggacgggc gaccgccggt cgagcggacg gggccggacg cctgcccgca
     9781 gtcaagacgc agccgacgcg gtcctacgac tgagcgagcg gggacgcgct ggtcacgtgc
     9841 gcggccggcg gcagctcagt cctcgccgag gtaggcgcgt ttcacccgat cgtcgcccag
     9901 tagttgctcg ccggtgccct ccagggtgat ctggccgacc tcgagcacat aggcccgatc
     9961 ggacagcttg agggccgcgg cggcgttctg ctcgaccagc agaatcgtga cgccctcgga
    10021 ctgcagtgca cggatcgtcg acatgatccg ctgcatcatc aggggcgaga ggcccatcga
    10081 cggctcgtcc agcatcagca gcttgggccg gctcatcatg gcccgtccga tggccagcat
    10141 ctgctgctcg ccgcccgaga aggtgccggc cggctgcgtg cgccgctcgt gcagaatctc
    10201 gaacaggtcg tagacgcggg tcaggtcctt ggcgatgccg tccgcgtcct tgcgcgcgaa
    10261 cgcgccgagc agcaggttgt cctcgacggt gagccgcgga aagatgcgcc gcccctcggg
    10321 ggagtgggcc aggcccctgg tcaccacctg gtgggccggg gtcttgtcga tgcgttcgcc
    10381 gttgaaccag atctcgcccg attcggcacg ttgcagaccc gagatggtgc gcagggtggt
    10441 ggtcttgccg gcaccgttgg tgcccagcag cgacaccacc tgcccctgtt caacggtgaa
    10501 gctgatgccc ttgaccgccc gcaccttgcc gtaggcgacc accaggtcct tgatctcaag
    10561 cattgccggc ctcgtcttcg gtctcgggag acccgatgta ggcctcgatc acccgcggat
    10621 cggactgcac ctcggcggcc gtgccctcga tcagggtctt gccgcgcacc aggcacagca
    10681 cccggtcgca caggttgaag atgaaccgca tgtcgtgctc gatcacgacg atggccaggc
    10741 ccgagtcgcg gatcttgaag atcaactctt cggcctgccg ggtctcttgg gggttcatgc
    10801 cggccgtcgg ctcgtccagc agcaacagtt tggggtcggt ggccagggcg cgggcgatct
    10861 cgagtcgccg ttggtcgccg tagggcaggt tgcgcgccag gtgtgcgccc tggcccttca
    10921 tgcccacgta ctcgagcagt tcggacgccc ggtcgcgggt ctcctgctcc tcgcgacgga
    10981 acttgggccc gcgcacgatc gaggtgagcg ccatcgcgga cgtgcggcag taccgtccga
    11041 ccatgacgtt ctccaacgcc gtcatgttgg ggaacagccg gatgttctga aacgtgcgcg
    11101 ccatgccggc caccaccacc ttgcgcggct tggggggcag cacctcaccg gcgaaccgca
    11161 cctcaccaga ggtgggtttg tacatgccgg tcaggcagtt gaagaaggtg gtcttgccgg
    11221 caccgttggg gccgatcagc ccgacgatct caccggtgtg cacgctgaag tcgacgttgt
    11281 cgaccgccac cagaccgccg aagcgcatgg tgacgttgcg ggcggcgaga atctcttcgc
    11341 ggcccgcacg atgctcgggg gccgccacgc tcactgcgtc ggtgctcatg acttctcctc
    11401 ggcgaactcg tagggcaggt cttcttcgac ctcttcgacc aactcctcat cgtcggagtg
    11461 gaactcgagg gcgcgtcgct ggttgggaat catgccctcc ggacggaccc gcatcatcac
    11521 caccatcagc aaaccgaaga gcaacatgcg gtactcgttg acgaagcgca gcttctcggg
    11581 caccagcttg agcagcgtgg cgcccaggat cacgcccatc accgtgccca tgccacccag
    11641 caccacggcg gccagcagga aggccgactc gagaaagatg tactgatcgg gcgtcaccga
    11701 ctggtcgacg tgagccttga ccgagccggc caggccggcc aggaacgcgc cgccggcgaa
    11761 ggcgagcagc ttgaggccga acacgttgac gcccatcgcc tcggcggcct tctcgtcctc
    11821 gcgaatggcg acccagcccc gtccgatgcg ggaatggttg agccgggtga agatcagcac
    11881 gatcagcccc atcagcagca acatcaagaa gaagtagttg ctgaaccggc ccaggggaat
    11941 gccgagcacg gtgtgcacct cgccgaagtc gaagccgaac aggttcaggt tgggaattgc
    12001 gggaatgccg gacgatccgt gcaccagatc ggggccgtcc tcgccgtcca ggttgttcat
    12061 cgtgatgcga aagatctcgc cgaacgcgag cgtgacgatg gcgaggtagt cgccggagac
    12121 ccgcagggtg ggggagccga tcaacaggcc gaggctggcc gagatcaggc ccgcgatcaa
    12181 catcaccacg atgaagggcg gatgccagtc gatggtggcg aacgccgact cgctcagggt
    12241 ggcggcggtg aacgcgccgg cccccagaaa cgcgatgtag ccgaggtcga gcaggccggc
    12301 caggccgacc acgatgttga gcccgacggc ggtggcggcg aacagcagca cctgcgtcgc
    12361 gatcgacatg ttcgcgtccg agccgttctg ggtaaacggg aacagaaagg cgacggtgaa
    12421 ggacgagaac accagcaccg tgcgatgctt ctggccggcg cgcgacagcc agccgcccaa
    12481 gccgctacgc atgatcacgg cagtgatcgt ggcgacgaac acaatgatgg ccaggaacgt
    12541 gccgccgtcg gtctggttca acgcgtacgc ggtgagcagc agggcgaaca gcagcagcag
    12601 agcgatggcc acgatctcga cccaggccgc cgaccggagc cgctccaggc cgggcgtgcg
    12661 gccgggggtc agcagcttgc tgccggtgaa gcccagcagg gcgccgatca gcgccaccca
    12721 gccgccccat tcgacggtga ccaggccacc gagttcgagt gcgatggcca gcaggatgaa
    12781 gccgatgtag gcgagcacac cgatgccggc cgccttcgtg ccgcgggtcc agccgacccg
    12841 cttgctgacc ttgagcacag cgggcgcgaa gccgcgt
//
LOCUS       DAOSUG010000016        12770 bp    DNA     linear   ENV 10-JUN-2024
DEFINITION  MAG TPA_asm: Micropruina sp. isolate AMR_MDS_2195
            NODE_2889_length_12770_cov_4.673299, whole genome shotgun sequence.
ACCESSION   DAOSUG010000016 DAOSUG010000000
VERSION     DAOSUG010000016.1
DBLINK      BioProject: PRJNA925477
            BioSample: SAMN32864262
            Sequence Read Archive: SRR4236673
KEYWORDS    WGS; ENV; Metagenome Assembled Genome; MAG; Third Party Data; TPA;
            TPA:assembly.
SOURCE      Micropruina sp. (wastewater metagenome)
  ORGANISM  Micropruina sp.
            Bacteria; Bacillati; Actinomycetota; Actinomycetes;
            Propionibacteriales; Nocardioidaceae; Micropruina.
REFERENCE   1  (bases 1 to 12770)
  AUTHORS   Abdulkadir,N., Saraiva,J.P., Zhang,J., Stolte,S., Gillor,O.,
            Harms,H. and Rocha,U.
  TITLE     Genome-centric analyses of 165 metagenomes show that mobile genetic
            elements are crucial for the transmission of antimicrobial
            resistance genes to pathogens in activated sludge and wastewater
  JOURNAL   Microbiol Spectr 12 (3), e0291823 (2024)
   PUBMED   38289113
REFERENCE   2  (bases 1 to 12770)
  AUTHORS   Abdulkadir,N.
  TITLE     Direct Submission
  JOURNAL   Submitted (24-MAR-2023) Environmental Microbiology, Helmholtz
            Center for Environmental Research -UFZ, Permoserstr 15, Leipzig
            04318, Germany
COMMENT     The annotation was added by the NCBI Prokaryotic Genome Annotation
            Pipeline (PGAP). Information about PGAP can be found here:
            https://www.ncbi.nlm.nih.gov/genome/annotation_prok/
            
            ##Genome-Assembly-Data-START##
            Assembly Method        :: SPAdes v. 3.11.1
            Genome Representation  :: Full
            Expected Final Version :: Yes
            Genome Coverage        :: Not Applicable
            Sequencing Technology  :: Illumina MiSeq
            ##Genome-Assembly-Data-END##
            
            ##Genome-Annotation-Data-START##
            Annotation Provider               :: NCBI
            Annotation Date                   :: 05/04/2023 04:57:10
            Annotation Pipeline               :: NCBI Prokaryotic Genome
                                                 Annotation Pipeline (PGAP)
            Annotation Method                 :: Best-placed reference protein
                                                 set; GeneMarkS-2+
            Annotation Software revision      :: 6.5
            Features Annotated                :: Gene; CDS; rRNA; tRNA; ncRNA
            Genes (total)                     :: 2,776
            CDSs (total)                      :: 2,750
            Genes (coding)                    :: 2,714
            CDSs (with protein)               :: 2,714
            Genes (RNA)                       :: 26
            tRNAs                             :: 24
            ncRNAs                            :: 2
            Pseudo Genes (total)              :: 36
            CDSs (without protein)            :: 36
            Pseudo Genes (ambiguous residues) :: 0 of 36
            Pseudo Genes (frameshifted)       :: 2 of 36
            Pseudo Genes (incomplete)         :: 32 of 36
            Pseudo Genes (internal stop)      :: 2 of 36
            ##Genome-Annotation-Data-END##
FEATURES             Location/Qualifiers
     source          1..12770
                     /organism="Micropruina sp."
                     /mol_type="genomic DNA"
                     /submitter_seqid="NODE_2889_length_12770_cov_4.673299"
                     /isolate="AMR_MDS_2195"
                     /isolation_source="Activated sludge of municipal
                     wastewater treatment plants"
                     /db_xref="taxon:2737536"
                     /environmental_sample
                     /geo_loc_name="Canada"
                     /lat_lon="49.25 N 123.1 W"
                     /collection_date="2015-06-16"
                     /metagenome_source="wastewater metagenome"
                     /note="metagenomic"
     gene            complement(<1..842)
                     /locus_tag="PLF56_01060"
     CDS             complement(<1..842)
                     /locus_tag="PLF56_01060"
                     /inference="COORDINATES: protein motif:HMM:NF019310.2"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_function: GO:0022857 - transmembrane transporter
                     activity [Evidence IEA];
                     GO_process: GO:0055085 - transmembrane transport [Evidence
                     IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="MFS transporter"
                     /protein_id="HOQ52182.1"
                     /translation="MAEPIAADVEHQALSSRDRSVIALLLVAAFVVILNETALNVALS
                     SIMVDLRVDERTVQWLTTAFMLTMAVVIPVTGWLLERIPTRSAFVLAMSLFTAGTLVC
                     AVAPIYALVLLGRIIQASGTAIMLPLLMTTVMQLVPPAHRGAIMGNISLVISVAPAVG
                     PTMAGIVLQFTSWRGVFALMVPIGLAMLIVGALRVVNVNELIRVPLDGWSIPLTVCGF
                     GGLVYGLSLIGDDTVPAFEVVAAFTVGALALVAFVWRQLLLARHERALLDLRAFRHRA
                     FTISV"
     gene            1035..1433
                     /locus_tag="PLF56_01065"
     CDS             1035..1433
                     /locus_tag="PLF56_01065"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_013864136.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="VOC family protein"
                     /protein_id="HOQ52183.1"
                     /translation="MTVSVSRCFVTVLDADEALRFYRDLLGLEVRLDVARGGFRWITV
                     GAPDQPGVDLVLSNYVQGSPDDLDTMRMMLAKGSLNAVHFRSGDLDEVFARLRDAGVE
                     VLDEPTDQPWGVRDCAVRDPAGNLIRIDQG"
     gene            complement(1564..2151)
                     /locus_tag="PLF56_01070"
     CDS             complement(1564..2151)
                     /locus_tag="PLF56_01070"
                     /inference="COORDINATES: ab initio
                     prediction:GeneMarkS-2+"
                     /note="Derived by automated computational analysis using
                     gene prediction method: GeneMarkS-2+."
                     /codon_start=1
                     /transl_table=11
                     /product="hypothetical protein"
                     /protein_id="HOQ52184.1"
                     /translation="MVDPAVLIAAWPLPARAELAEQLLGRWREPHRHYHGVQHLAECL
                     AAADELGAGVAERLALWFHDAVHTNTRGSDEAASAALAALLLADHLPAVVVDEVTRLI
                     LLTIHHQPLAGDRPGAIVCDADLWVLGAAPRRYQQSVRQLRAEFGSSAERWTKLRRDQ
                     LRERLAGPIFHGPNQAAREARARDNLQIELSAMEG"
     gene            complement(2130..2558)
                     /locus_tag="PLF56_01075"
     CDS             complement(2130..2558)
                     /locus_tag="PLF56_01075"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_012918617.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="hypothetical protein"
                     /protein_id="HOQ52185.1"
                     /translation="MTVWLVVAKALIVLVLAAAAVLLGGRVVTRVFRFAERTRAAELP
                     ASDEPGSTEAIAPLTHAAAYLRGGEWIGYLERLAAFGTLLAGFPEGVAVAMVVKSFAR
                     YPEIKASSTGAAERFIIGTFVSVLFAAACAGLAQWLIRLF"
     gene            complement(2555..3211)
                     /locus_tag="PLF56_01080"
     CDS             complement(2555..3211)
                     /locus_tag="PLF56_01080"
                     /inference="COORDINATES: ab initio
                     prediction:GeneMarkS-2+"
                     /note="Derived by automated computational analysis using
                     gene prediction method: GeneMarkS-2+."
                     /codon_start=1
                     /transl_table=11
                     /product="transposase"
                     /protein_id="HOQ52186.1"
                     /translation="MQHEDLTLWAVVTADQRNSRRSSDHVPDALTALQAVVGDRFALG
                     FERTAGDELQGLTRDPGAVVDAVLTLTEQPFWHVGIGIGEIEQPLPASTREARGPAYV
                     AARAAVEQARSAPAHLRLVAAETVGPSSYGETVAQRAERAESALVLLRALVSRRTPEG
                     WEIMQVLDETGSGRAAAERLGISASAVSQRATRSARAESLAGAELARVLLAEALAVPA
                     "
     gene            3376..3645
                     /locus_tag="PLF56_01085"
     CDS             3376..3645
                     /locus_tag="PLF56_01085"
                     /inference="COORDINATES: protein motif:HMM:NF016141.2"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_component: GO:0016020 - membrane [Evidence IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="GlsB/YeaQ/YmgE family stress response membrane
                     protein"
                     /protein_id="HOQ52187.1"
                     /translation="MLSWGFLGWIILGGIAGWLASKIMKTDAQQGIIMNIIVGIVGGL
                     LGGWVLSLFGVNVAGGGLIFSMLTAIGGAVLLLWIVGMVTGRRSA"
     gene            3806..5167
                     /locus_tag="PLF56_01090"
     CDS             3806..5167
                     /locus_tag="PLF56_01090"
                     /EC_number="1.-.-.-"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_013160839.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="NAD(P)/FAD-dependent oxidoreductase"
                     /protein_id="HOQ52188.1"
                     /translation="MSDEYDVIVIGAGPVGENAAQYAIRGTEYRAVLIERELVGGECS
                     YYACMPSKALLRPIAVAGATGDLRGVRPTRIDPADLLARRDYWVSHYDDSGQVSWAES
                     AGIDVVRGEARVSGERQVTVGERTLTARHAVVLATGSRAAIPSELASVHPWTSRDATG
                     VVEIPPRLAIVGGGVVACEAATWMAALGSRVTLLVRSSLLPRLERFAADEVQDGLRRA
                     GVEVRLGVAVAQAQRPGEVTDDLGRPHGSPVRLLTDGGWEEFDEVLVATGRRLSTDAL
                     PEHLRPSAGATPRLPDWLHLVGDVSGEPALTHWGKYRARRLGARLQAELDGRALPPEP
                     DGVPVPQVIFTDPEVATVGPTLAQAQADDAQAYALDVPIESASGVGLLIDHPHGRARL
                     VVAGDGRLVGATFVGPEVAEMLHAATVAIVGRMTLDQLWHAVPSYPTASEVWLRLLES
                     TLP"
     gene            complement(5290..5649)
                     /locus_tag="PLF56_01095"
     CDS             complement(5290..5649)
                     /locus_tag="PLF56_01095"
                     /inference="COORDINATES: ab initio
                     prediction:GeneMarkS-2+"
                     /note="Derived by automated computational analysis using
                     gene prediction method: GeneMarkS-2+."
                     /codon_start=1
                     /transl_table=11
                     /product="hypothetical protein"
                     /protein_id="HOQ52189.1"
                     /translation="MTTTSETTIYDWQAALLAFNTRDYKTAIDGFEKVLSAQPGHTDA
                     REYLARAHYQRASLVPAEREARAILAADPTNEYVTLLLARTLERQSRHEEAAGVRRVL
                     VALTGDARHAATHAALG"
     gene            complement(5654..6673)
                     /locus_tag="PLF56_01100"
     CDS             complement(5654..6673)
                     /locus_tag="PLF56_01100"
                     /EC_number="1.-.-.-"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_011756441.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_function: GO:0016491 - oxidoreductase activity
                     [Evidence IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="LLM class flavin-dependent oxidoreductase"
                     /protein_id="HOQ52190.1"
                     /translation="MTKKIGFLSFGHYRDVPGSRVRTAGDSLLQHIDLAVAAEAAGLD
                     GAWLRVHHFDHNLGAPFPLLSAMAARTSTIEVGTGVVDLRYENPLYLAEEAGATDLIS
                     AGRLQLGISRGSPEPAADGQAAFGYRLAEGEDWSSVTRARAELFRRAIGGHPVARSQR
                     ALDRDAAPDLTVQPLSPGLPNRIWWGAGTHATGVWAGRAGYNLLSSTLLLADDGRPFH
                     LQQADQVRDYRAALAESGHRTGGQAAVTRSAFPITTAEDQSYFGLDRGDTDSAGILDG
                     LPARSGPTFAGSIDEVATGLAADEAVREADYVLFALPSMLGVDYNAHLFENLVALARE
                     LGWKE"
     gene            complement(6743..7399)
                     /locus_tag="PLF56_01105"
     CDS             complement(6743..7399)
                     /locus_tag="PLF56_01105"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_007079334.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_function: GO:0008237 - metallopeptidase activity
                     [Evidence IEA];
                     GO_function: GO:0016805 - dipeptidase activity [Evidence
                     IEA];
                     GO_process: GO:0006508 - proteolysis [Evidence IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="M15 family metallopeptidase"
                     /protein_id="HOQ52191.1"
                     /translation="MQPGVVLISDPRVVGIPVRDCGDPLIDLSGLPRLRIDARKHSAA
                     GGWHWARSGLAERLSSAAQSLPADLELLIVEGYRAPSRQREYWDHYCDELREHYPGLN
                     HERLYDLASRWVAPPDVAPHCTGGAVDLTLCEADGRELDLGTPLDATPEQSDGACYTA
                     ASVPGPAATNRATLVRALSGAGLVNYPTEWWHWSYGERYWAFMTGAERARYGPIVWPE
                     "
     gene            complement(7505..8179)
                     /locus_tag="PLF56_01110"
     CDS             complement(7505..8179)
                     /locus_tag="PLF56_01110"
                     /inference="COORDINATES: ab initio
                     prediction:GeneMarkS-2+"
                     /note="Derived by automated computational analysis using
                     gene prediction method: GeneMarkS-2+."
                     /codon_start=1
                     /transl_table=11
                     /product="hypothetical protein"
                     /protein_id="HOQ52192.1"
                     /translation="MSANQPPENVNNPQQIPGSGPQNPQSASEGQPGHPGDAAPQVGQ
                     PGQPAQLGYGGPQVGQPGQPGQLGYGGPQVGPDGQPVQPGYAGPPLGPDGQPLPPGAY
                     PPPGGFQPQVAEQPKKGFQLKKFLTSIGIAIVVIVLGIMWRTGVFDSPSMKVGDCVQQ
                     TGSDSVKVVDCGSADAQYKVLGIKEKQSQSAGRLGACNDWPDTTSIYWEGRNTASGTV
                     YCLQQV"
     gene            complement(8327..8554)
                     /locus_tag="PLF56_01115"
     CDS             complement(8327..8554)
                     /locus_tag="PLF56_01115"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_005179144.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="DUF2945 domain-containing protein"
                     /protein_id="HOQ52193.1"
                     /translation="MAAHTVAAITISKGTRVSWNTSQGRTQGEAVERRTHGFQFDGQQ
                     FNASDDEPYWIVRCEKTGAEAAHKASSLREL"
     gene            complement(8650..9111)
                     /locus_tag="PLF56_01120"
     CDS             complement(8650..9111)
                     /locus_tag="PLF56_01120"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_014174167.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="VOC family protein"
                     /protein_id="HOQ52194.1"
                     /translation="MGREFQVTFDCHDPAALSLFWRDALGYVHPAPPGVELGEGDDPL
                     VAWDQFLDRMDVPVEQRNRSSALQDPAGRGPRIFFQQVPEGKVAKNRMHLDVRAAPGL
                     KGEERLSALEAECSRLLALGATRVQRFEPEPYLSDGFIVMRDPEGNEFCLD"
     gene            9635..10888
                     /locus_tag="PLF56_01125"
     CDS             9635..10888
                     /locus_tag="PLF56_01125"
                     /inference="COORDINATES: protein motif:HMM:NF015056.2"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="acetamidase/formamidase family protein"
                     /protein_id="HOQ52195.1"
                     /translation="MPEHVFKLDNSKRFTDQEKLGHNRWHPEIPPVATVKPGDSFRVD
                     CREWFDGAIKNDDSAQDILDAPLLTVHTLSGPFAVEGAKPGDLLIVDILDVGPCPQED
                     SGPLAGQGWGYTGIFATRNGGGFLTEQFPDAYKAIWDFTGQVATSRHVPGVSYTGLIH
                     PGLMGTAPSAELLATWNAREGALIATDPDRVPPLALPPEAEHAILGGLDRSEWARVGG
                     EAARTAPPRENGGNQDIKNMSKGTRIFYPVFVDGANLSLGDLHFSQGDGEITFCGAIE
                     MGGFIDLRVDLIKGGMDTYGVAENAIFIPGNVDPQYSEWIAFSGTSVTLGGEQRYLDS
                     HLSYQRACLHAIDYLTKFGYSPEQAYLLLGSAPIEGRLSGVVDIPNSCSTVYIPTAIF
                     DFDVRPSASGPVQIDPGKGAPRASA"
     gene            complement(10979..11971)
                     /locus_tag="PLF56_01130"
     CDS             complement(10979..11971)
                     /locus_tag="PLF56_01130"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_012922629.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="hypothetical protein"
                     /protein_id="HOQ52196.1"
                     /translation="MPLVRLISLALVVVLAGCGTAPPTGPAPSVSAPAPTTASATDAA
                     TSPTAAPQTSQPPAPSDLPRGGRELFPKYRLFGYSGFPGAPGQGRLGIGNLDDRMKEI
                     EQRGQAFRGGRELLPVMELIATTVHGSPGADGKFRTRVDDKVIKQWLATADKHKALLL
                     LNIQPGRADFIDEVKYFAKWLAYPNVGLALDPEWAVDKGQIPGRVFGHTSGKELDQVA
                     SYLSGVVAANNLPQKVLLFHQLNRGVVKNPKDLKQYPGVVYINSVDGIGPPGAKVSTY
                     NAVIKTRPTYVHAGFKLFYVEDKLPRYNGGKTGRMMTAKEVLKLKPQPEYILYE"
     gene            complement(12268..>12770)
                     /locus_tag="PLF56_01135"
     CDS             complement(12268..>12770)
                     /locus_tag="PLF56_01135"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_002536884.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=3
                     /transl_table=11
                     /product="dihydrolipoyl dehydrogenase"
                     /protein_id="HOQ52197.1"
                     /translation="RTNVPGVYAIGDVTMKLALAHVAEAQGVVAAETIAGAETMAINY
                     DMIPRATYCQPQIGSFGYTEEQAKQRGYDVKVSKFPFSANGKAWGLGDATGFVKIIAD
                     AEHNEILGAHMIGPDVTELLPELTLAQQWDLTADEVGRNVHAHPTLSEALKEAVEGIA
                     GHMINF"
BASE COUNT         2000 a         4362 c         4365 g         2043 t
ORIGIN      
        1 acgctgatcg tgaaggcgcg atgccgaaag gcgcgcaggt cgagcagcgc gcgctcgtgc
       61 cgggccaaca gcaactggcg ccacacgaag gcgaccaacg ccagcgcgcc caccgtgaag
      121 gccgccacca cctcgaacgc cggcacggtg tcgtcgccga tcaggctcag gccgtagacc
      181 agaccgccga agccacaaac ggtcagcgga atcgaccacc cgtccagggg cacgcggatc
      241 aactcgttga cgttcaccac ccgcagcgcc ccgacgatca gcatggccag gccgatcggc
      301 accatcaggg cgaacactcc gcgccagctg gtgaactgca gcacgatgcc tgccatggtg
      361 gggccgaccg cgggggccac cgagatcacc agcgagatgt tgcccatgat cgcgccccgg
      421 tgcgcgggcg gaaccagttg catcacggtg gtcatcagca gcggcagcat gatggctgtt
      481 ccgctggcct gaatgatgcg cccgagcagc accagggcat agatcggggc gacggcgcag
      541 accagggtgc cggcggtgaa caggctcatg gcgagcacaa acgcgctgcg ggtcgggatg
      601 cgttcgagca accagccggt gaccggaatc accaccgcca tggtcagcat gaaggccgtg
      661 gtcagccact gcaccgtccg ttcgtcgacg cgcaggtcga ccatgatgga actgagtgcc
      721 acgttgagcg ccgtttcgtt gagaatcacc acgaacgccg cgaccagcag cagggcgatc
      781 acggagcgat cccgcgaact cagcgcctgg tgctcgacat cggctgcgat cggctcggcc
      841 atggcgatcc cctttgcttg ggcgaagggg tcacgctacg gcccggcaac gggcggttcc
      901 aaattcctac cacgatgacg atggccggct ggttcattcg cggacgactc gtagcagcag
      961 tacggacgcc ggggcgtccg acgttgaggg gtccgggggg tgcgcctgga ggtgtgtccc
     1021 ctagggtggc gcgcatgacc gtctcagtct cacgctgctt cgtcaccgtg ctcgacgcgg
     1081 acgaagccct ccgcttctac cgcgacctgc tgggcctcga ggtgcggctg gacgtggccc
     1141 ggggtgggtt tcgctggatc accgtcggcg ctccggacca gccgggcgtc gatctggtgc
     1201 tcagcaacta tgtgcagggt tcgccggacg acctcgacac catgcggatg atgctcgcca
     1261 agggttctct caacgcagtg cattttcgct ccggcgacct ggacgaggtg ttcgcccggc
     1321 tgcgcgatgc cggcgtcgag gtactggacg aacccacgga tcaaccctgg ggggttcgcg
     1381 actgcgccgt gcgcgatccg gccggcaacc tgatccggat cgatcagggc tgagcccgga
     1441 tcggccgatg tgcgcctgct gtgggctagg ccgtccgggc gaggccatcc cgggcgtctg
     1501 gtcagcgcgg tggcgaacga caccgtgcag ccgctcgcca cgccggccgg gttcgcccac
     1561 agctcagcct tccattgcgc tcagttcgat ctggaggttg tctcgtgcgc gggcctcacg
     1621 cgcggcctgg ttcgggccgt gaaagatcgg cccggccaat cgctcgcgca gctgatcgcg
     1681 ccgcagcttc gtccatcgct cggcgctcga gccgaactcc gctcggagtt gccgcaccga
     1741 ctgctggtaa cgccgcggcg cggcgcccag cacccacagg tcggcgtcgc acactatcgc
     1801 tcccggtcga tcgccggcca ggggttgatg atggatcgtc aacaggatca gccgggtcac
     1861 ctcgtcgacg acaactgcgg gcaggtgatc ggccagcagc agagccgcca gtgcggccga
     1921 ggcggcctcg tcactgccgc gggtgttggt gtgcaccgcg tcgtgaaacc acagcgcgag
     1981 tcgctcggcg acccctgccc cgagctcgtc ggcagccgcc aggcattctg cgagatgctg
     2041 cacgccgtgg tagtgccgat ggggctcgcg ccaccggccg agcagctgct cggccagctc
     2101 ggcgcgcgca ggcagcggcc acgccgcgat cagaacagcc ggatcaacca ctgcgccagc
     2161 cctgcacacg cagcagcgaa cagcacgctg acgaacgtgc cgatgatgaa tcgttcggca
     2221 gctccggtgc tgctggcctt gatttcgggg taccgcgcga agctcttgac caccattgcc
     2281 accgccacgc cctcgggaaa acctgccagc agcgtgccga aggcggcgag gcgttcgagg
     2341 tacccgatcc attcgccgcc gcgcaggtag gccgcggcgt gggtgagagg cgcgatcgcc
     2401 tcggttgagc cgggctcgtc ggatgcgggc agctcggcgg cccgggtgcg ctcggcgaac
     2461 cggaagaccc gggtgaccac gcgtccaccc agaagcaccg ccgctgccgc gagcacgagc
     2521 acgatcaggg ccttggccac caccagccac acggtcatgc cggcaccgcc aaagcctcgg
     2581 cgagcaacac ccgcgccagc tcggcccccg ccagcgactc ggcgcgcgcc gacctggtgg
     2641 cccgctgcga caccgccgag gcgctgatgc ccaggcgttc ggcggcggcg cggccgctgc
     2701 cggtctcgtc gagtacctgc atgatctccc aaccctccgg ggtgcgacgg ctgaccagtg
     2761 cccgcagcag caccagagcg gactcggcgc gttcagcgcg ctgcgcaacg gtttcaccat
     2821 aggacgacgg gccgacagtt tcggctgcaa cgagccgcag gtgcgccggc gccgatcgcg
     2881 cctgttcgac cgccgcgcgg gccgccacgt acgccggccc ccgcgcctcg cgggtcgagg
     2941 cgggcaacgg ctgctcgatc tcgccgatac cgatgccgac gtgccagaac ggttgttcgg
     3001 tgagtgtgag gacggcatcc accaccgcgc ccgggtcgcg ggtcagcccc tgcaactcgt
     3061 caccggccgt gcgctcgaag cccaacgcga agcggtcgcc gacgacggcc tgcaacgccg
     3121 tcagcgcgtc gggcacgtga tccgacgagc gtcggctgtt gcgctggtcc gcggtcacca
     3181 ccgcccagag ggttaagtct tcatgctgca tttccactca ttaagtttgc atgctgaaca
     3241 gactgatgtc cagtcaccct gaccacctca cccctagggg ttaagggtgc gattcggggc
     3301 gttccctggc gcgtgttggc gcacctcgcg cctagagtgg gcgaggtcaa accagcacga
     3361 gagaaggagc aacacatgtt gagttgggga tttctgggct ggatcatcct gggcggtatc
     3421 gccggttggc tcgccagcaa gatcatgaag accgacgccc aacagggcat catcatgaac
     3481 atcatcgtgg gcatcgtcgg cggcctgctc ggcggctggg tgctcagtct cttcggcgtg
     3541 aacgtcgctg gtggtggcct gatcttcagc atgttgacgg ccatcggcgg cgcggtgctg
     3601 ctgttgtgga tcgtcggcat ggtcaccgga cgcagatccg cctgatcatc gcaccgagca
     3661 gggccggcat ccttcacgga tgccggccct gctcgctgtt tgcggccgat cggtacgcgt
     3721 gccacggttc ggcagccgcg cagtggtgcg tcgggtggtc cattcggagg ttgcgcaccc
     3781 accggatggc cacgagcatg gcgccatgag cgatgagtac gacgtgatcg tgatcggtgc
     3841 cggcccggtg ggtgagaacg cggcgcagta cgcgatccgc ggcaccgagt accgggcggt
     3901 gttgatcgag cgcgagttgg tcggtggcga atgctcgtac tacgcctgca tgccgagcaa
     3961 ggcgctgctg cgtccgatcg cggtggccgg ggcgacgggt gacctgcgtg gggtgcgtcc
     4021 gacccgcatc gacccggccg acctcctggc ccggcgcgac tactgggtca gtcactacga
     4081 cgactccggg caggtgtcgt gggccgaatc cgcgggcatc gacgtggtcc ggggcgaggc
     4141 ccgcgtgagc ggcgagcggc aggtcacggt gggtgagcgc accctgaccg cccggcacgc
     4201 ggtggtgctc gccaccggaa gccgcgccgc gatcccgtcc gagctggcct cggtccaccc
     4261 ctggacgagc cgcgatgcca ccggcgtggt cgagatcccg ccgcggctgg cgatcgtcgg
     4321 cgggggagtg gtggcctgcg aggccgccac ctggatggcg gccctgggca gccgggtcac
     4381 cctgctggtg cgcagttccc tgttgcctcg cctggagcgg ttcgccgccg acgaggtgca
     4441 ggacgggctg cgccgcgccg gcgtcgaggt ccggctgggc gttgcggtgg cgcaggccca
     4501 gcgtccgggg gaggtgaccg acgatctcgg acgcccgcac ggcagcccgg tccggctact
     4561 caccgatggc gggtgggaag agttcgacga agtgctggtg gcgaccggcc gtcggctgag
     4621 tacggatgcg ctacccgagc acctgcgtcc aagcgcgggc gcgacgccgc ggttgccgga
     4681 ttggttgcac ctggtcggcg acgtgtccgg cgagccggcg ctcacccact ggggcaagta
     4741 ccgcgcccga cgactcggcg ctcgtttgca ggccgaactc gatggacgtg ccctgccccc
     4801 cgaaccggac ggcgtaccgg ttccgcaggt gatcttcacc gaccccgagg tcgccaccgt
     4861 cggacccacg ctggcgcagg cccaggccga cgatgcgcag gcgtatgcgc tcgacgtgcc
     4921 gatcgagtcg gcgtccggag tggggttgct gatcgaccat ccgcacggcc gggcgcggct
     4981 ggtcgtggcc ggcgacggac ggctggtggg cgccacgttc gtcggtcccg aggtggcgga
     5041 gatgctgcac gccgccacgg tggcgatcgt gggccggatg accctcgatc agctgtggca
     5101 tgccgtgccc agctatccga cggcgagcga ggtgtggctg cggttgctgg agtcgacgct
     5161 gccctgacgg tgcggtgggc gacgtcgcgc ggtcaagcgt tcgttgcgcg cgtccgtacc
     5221 cgcgcaacga accccgaacc gcgcaacgac gaaccggccc tgtcccccag gcctccggac
     5281 ggctgcggct cagcccagtg cggcgtgcgt ggccgcgtgt cgcgcatcac cggtcaacgc
     5341 gaccagcacc cggcgcacgc cggccgcttc ttcgtgccga ctctggcgct ccagagtgcg
     5401 cgccagcagc agcgtgacgt actcgttggt cggatcggcg gccagaatcg cccgcgcctc
     5461 acgctcggcg ggcaccagcg acgcccgctg gtagtgcgct cgcgccaggt actcacgcgc
     5521 gtcggtgtgg cccggctgcg cgctcagcac cttctcgaac ccgtcgatgg cggtcttgta
     5581 gtcgcgggtg ttgaaggcga gcagcgccgc ctgccagtcg tagatcgtgg tctcggacgt
     5641 ggtggtcatc gggttactcc ttccagccca gttcgcgcgc aagtgcgacc aggttctcga
     5701 acaggtgggc gttgtagtcg acgcccagca tggacggcag cgcgaacagc acgtagtcgg
     5761 cttcgcgtac cgcttcgtcg gcggccaggc cggtcgccac ctcgtcgatg ctgccggcga
     5821 acgtggggcc ggaccgggcc ggcagcccgt ccaggatgcc cgcggagtcc gtgtcgccgc
     5881 ggtccagccc gaagtaggac tggtcttcgg cagtggtgat cgggaacgcg ctacgggtca
     5941 ccgccgcctg gccgccggtg cggtggccgg actcggccag ggcggcccgg tagtcgcgca
     6001 cctgatcggc ctgctgaagg tggaacggac gtccgtcatc ggccagcaac aaggtcgaac
     6061 tcagcaggtt gtaccccgct ctgcccgccc agacgccggt ggcgtgcgta cccgcacccc
     6121 accagatgcg gttcggcagc ccgggggaga gcggctgcac ggtcaggtca ggcgcggcgt
     6181 cacgatcgag cgcccgctgc gaacgcgcca ccgggtggcc gccgatcgcc cgacggaaca
     6241 gctcggcgcg ggcgcgggtc accgacgacc agtcctcgcc ctcggccagc cggtagccga
     6301 acgcggcctg cccgtcggcg gccggctcgg gcgatccccg gctgatgccg agttgcagac
     6361 gtccggcgct gatcaggtcg gtcgcacccg cctcttcggc caggtagagc gggttctcgt
     6421 agcgcagatc gaccacaccg gtgcccacct cgatggtgga cgtgcgcgcc gccatcgctg
     6481 acagcagcgg aaacggtgcc cccagattgt ggtcgaagtg atgcacgcgc aaccaggcgc
     6541 cgtccagccc ggcggcctcg gcggccaccg ccaggtcgat gtgctgaagc agcgaatcac
     6601 ctgccgtgcg cacccgcgag ccgggtacgt cgcggtagtg cccgaaggac aggaagccga
     6661 tcttcttggt catcgccact ctcatcgatc agtcgccctc ggcgacatga atagttgtgg
     6721 tttcaacaac ttaccggcgg agttattccg gccacacgat cggcccgtag cgggcacgct
     6781 cggcaccggt catgaaggcc cagtaccgct cgccgtacga ccaatgccac cattcggtgg
     6841 ggtagttgac cagaccggcc ccactcagcg cacgaaccag ggtggcccgg ttcgtcgccg
     6901 ccggcccggg caccgaggca gcggtgtagc aggcgccgtc gctttgttcg ggggtcgcat
     6961 ccagcggcgt gccgagatcg agttcgcggc cgtccgcctc gcacaacgtc aggtcgacgg
     7021 caccaccggt gcaatgtggc gcgacgtccg ggggagccac ccagcggctg gccagatcat
     7081 agagtcgctc gtggttcaag ccggggtagt gctcgcgaag ctcatcgcag tagtggtccc
     7141 agtactcccg ctgccgggac ggggcccggt acccctcgac gatcagcaac tcgagatcgg
     7201 ccggaagcga ctgggcggcc gacgacagcc gctcggccag tccgctccgc gcccagtgcc
     7261 agccaccggc agcactgtgc ttgcgcgcat cgatgcgcaa tcggggcagc ccgctcaggt
     7321 cgatgagggg atccccgcag tcgcgcacgg gaatgccgac cacgcgcgga tcactgatca
     7381 gcacgacccc aggttgcacc gcgccagtct ggcacggttc tgcaccctca acacgccgat
     7441 cggcatggcg gtggcgccga cccgagccgg ggtcggcgcc atcgaaaagg gcgcgcgctt
     7501 gcggtcagac ctgctgcagg cagtacacgg tgcccgaggc cgtgttgcga ccctcccagt
     7561 agatgctggt ggtgtcgggc cagtcgttgc aggcgccgag ccgtccggcg gactggctct
     7621 gcttctcttt gatgccgagt accttgtact gcgcgtcggc gctgccgcag tcgaccacct
     7681 tgacgctgtc gctgccggtc tgctgcacac agtcaccgac cttcatactg gggctgtcga
     7741 agacaccggt gcgccacatg atgcccagga cgatcaccac tatggcgatg ccgatcgagg
     7801 tcaggaactt cttcagctgg aagcccttct tgggctgttc ggcaacctgc ggttgaaaac
     7861 caccgggcgg tgggtacgcg ccgggcggca gcggctggcc gtccggaccg agtggagggc
     7921 cggcgtagcc gggctggacg ggctgaccgt ccggaccgac ctgcgggccg ccgtaaccga
     7981 gttgaccggg ctgaccgggc tgaccgacct gcgggccgcc gtaaccgagt tgggcgggct
     8041 gaccgggctg accgacctgc ggagcggcgt caccggggtg accgggctgg ccctcggacg
     8101 cggactgcgg gttctgcggg cccgacccgg ggatctgttg ggggttgttg acgttctcgg
     8161 gaggctggtt tgcgctcatg ggcgtccttt ctgagcgagc tttggcaggc cgtcagtctg
     8221 ccagtcgggg cgaccacgat cgtccacgca atggggggag tactgcccga ttccgtgagg
     8281 gcatccttct ggcgcttttg acataatgtt gcttatcgga caagtctcac agctctcgca
     8341 gcgacgacgc tttgtgcgcg gcctcggcgc cggtcttctc gcaccggacg atccagtacg
     8401 gctcatcgtc gctggcgttg aactgctggc cgtcgaactg gaacccgtgg gtacgtcgct
     8461 cgacggcttc gccctgcgtg cgcccctgcg acgtgttcca cgagacccgg gtgcccttac
     8521 tgatggttat ggcagcgaca gtatgcgccg ccaacgaccg ccggaaccac cccgccgggc
     8581 atcattcact gcccctccgg gtgaattgaa ggcctggcaa tgaatcggcg ccaatgaagg
     8641 ctggcgactt cagtcgaggc agaactcgtt gccctcgggg tcccgcatca cgatgaaccc
     8701 gtcgctcagg tatggctccg gctcgaaccg ttgcaccctg gtcgcaccca acgccagcag
     8761 gcggctgcat tccgcctcca gggcgctgag tcgctcctca cctttgagtc cgggggcagc
     8821 tcgcacgtcc aggtgcatcc gattcttggc caccttgccc tctggcacct gctggaagaa
     8881 gatccgcggg ccgcgccccg ccgggtcctg cagcgcggac gaccggttgc gttgctccac
     8941 gggcacgtcc atccggtcga gaaactggtc ccaggccacc agcgggtcat cgccctcccc
     9001 cagctcgacg cccggcggcg ccgggtgcac gtagccgagt gcgtcgcgcc aaaacagcga
     9061 gagtgccgcg ggatcatggc aatcgaaggt gacctgaaac tctctgccca tcggccgatt
     9121 atagaacgta gagccgaatt ggaacagagg gaaccattga cttcgccggg gtggccggat
     9181 agcgtcccgg aatagtcaca cgccgacgag cgaagatctg cagagcggca cctcttcgct
     9241 agcctcggca gcccgacttg ccccgcgtcc acccgcgagc aggaggcgat ccgtgcccac
     9301 atacagctat cggtgcgtca atggttgcac cttcgatgca gtctttgcca tggccgaggt
     9361 tccagcagaa ctgacgtgca cacggtgcgg cgtctcagcc cgccgggtga tcggcgcacc
     9421 gcatctggcc gcaaccggaa ccccggcctt ccaagctgtg gatgccgcgg cccgcagtgc
     9481 gcacgaaccc agcgtcgtca cgagtctgcc ttccaagggc cgccagcgag tacagcgggt
     9541 gactcacaac cccctgcatc agaaactgcc gcggcagtga cggcaacgcc acccgcgatg
     9601 atcacccagt aacaccaacc gaaggagcag caccatgccg gagcacgtgt tcaagctgga
     9661 caacagcaag cggttcaccg accaggagaa gctgggtcac aaccgctggc accccgagat
     9721 cccgccggtg gcaacggtga agcccgggga ctccttccgg gtggactgtc gcgagtggtt
     9781 cgacggggcg atcaagaacg acgactccgc ccaggacatc ctggacgcgc ccctgctcac
     9841 cgtgcacacc ctgagcggcc ccttcgcagt cgagggcgcc aagcccggcg acctgctgat
     9901 cgtcgacatc ctggacgtcg gcccgtgccc ccaggaggac tcggggccgc tcgccggcca
     9961 gggttggggc tacaccggca ttttcgccac ccgcaacggt ggcggcttcc tcaccgagca
    10021 gttcccggac gcctacaagg cgatctggga cttcaccggt caggtcgcca cgtcgcggca
    10081 cgttcccggt gtgtcctaca ccggcctgat ccatcccggc ctgatgggca ccgcaccgtc
    10141 cgccgaactg ctcgccacgt ggaacgcgcg cgagggcgca ctgatcgcca ccgacccgga
    10201 ccgggtgcca ccgctggccc tgccgcccga ggccgagcat gccatcctcg gcggcctcga
    10261 ccgctcggag tgggccaggg tcggcggtga ggcggcacgc accgcaccgc cgcgtgagaa
    10321 cggcggcaac caggacatca agaacatgag caagggaacg agaatcttct accccgtgtt
    10381 cgtcgatggc gccaacctgt ccctgggcga tctgcacttc tcccagggcg acggcgagat
    10441 caccttctgc ggtgcgatcg aaatgggtgg gttcatcgac ctgcgcgtcg acctgatcaa
    10501 gggcggaatg gacacctacg gggtggccga gaacgccatc ttcattccgg gcaacgtcga
    10561 tccgcagtac agcgagtgga tcgcgttctc tggcacgtcg gtcaccctgg gtggcgaaca
    10621 gcgctacctc gattcgcatc tgtcctacca gcgcgcctgc ctgcacgcga tcgactacct
    10681 gaccaagttc ggctacagcc ccgagcaggc ctacctgctg ctgggctcgg caccgatcga
    10741 agggcgcctg tccggcgtgg tggacattcc caactcgtgc tcgacggtct acatcccgac
    10801 cgcgatcttc gacttcgacg tgcggccgtc ggcctcggga cccgtacaga tcgatcccgg
    10861 caagggagca cccagggcgt ccgcctgagc cggcgggcga tgtgggtcgg ctgcagacgc
    10921 cgacccacat cgtccgatca ccggcatcgg tggggcgggg tgctgggttt cgcaccgctc
    10981 attcgtacag gatgtattcc ggctgcggct tgagcttgag cacctctttg gccgtcatca
    11041 tgcgaccggt cttgccaccg ttgtaccggg gcagcttgtc ctcgacgtag aacagcttga
    11101 agcccgcgtg gacgtacgtg ggtcgggtct tgatcaccgc gttgtaggtg gacaccttgg
    11161 caccgggcgg gccgatgccg tccaccgaat tgatgtagac cacgccgggg tactgcttga
    11221 ggtccttggg gttcttgacc accccacggt tgagctggtg gaacaacagc accttctgcg
    11281 gcaggttgtt ggcggccacc acgccggaaa ggtaggacgc cacctggtcg agttccttgc
    11341 cgctggtgtg gccgaacacg cgacccggga tctggccctt gtccacggcc cactcggggt
    11401 caagggccaa ccccacattc gggtacgcga gccatttggc gaagtacttc acctcatcga
    11461 tgaagtcggc ccggccgggc tgaatgttca gcagcagcag cgccttgtgc ttgtcggcgg
    11521 tggcgagcca ttgcttgatc accttgtcgt cgacgcgggt gcggaacttg ccgtccgccc
    11581 cgggtgaacc gtgcacggtg gtggcgatca gttccatcac cggaagcagt tcgcggccac
    11641 cgcggaaggc ttggccgcgc tgttcgatct ctttcatgcg gtcgtcgaga ttgccgatgc
    11701 ccagccggcc ctgaccgggc gcacctggaa agcccgagta gccgaacaac cggtacttcg
    11761 ggaacagctc acgtccgcct cggggcagat cggacggggc gggtggctgt gacgtctgcg
    11821 gtgccgcagt tggcgacgtg gccgcatcgg tggcggatgc ggtcgtggga gcgggtgctg
    11881 ataccgacgg ggcgggcccc gtggggggcg ctgtgccgca accggccagc accaccacga
    11941 gggcgagcga gatcaagcgg acgaggggca ttgcttcatt gtgcctgctg attgcacagc
    12001 ctccgcacag ctaccggcgc gcgttcaggc aatcatcagt caatcgtcac ccggtcgtca
    12061 tgcggcctgg cgcagcgacg acctcgaccc gtcggttctt ccgcttgccc gcatccgtcc
    12121 agttggacgc gaccggccgg gtctcaccga agccggaccc cgtcatcccg cgacccaccc
    12181 cgccggagcc agtgcgtccg cggaacacct cggggcggac gcagcaccgc cgcccggagt
    12241 tgaacccggc gtcggtcttc ctgcggctca gaagttgatc atgtgcccgg cgatgccctc
    12301 aaccgcctct ttcaacgcct ctgagagggt cgggtgcgcg tgcacgttgc gtccgacctc
    12361 gtcggcggtc aggtcccact gctgggccag ggtcaactct ggcagcagtt cggtgacgtc
    12421 ggggccgatc atgtgcgcgc ccaggatctc gttgtgctcg gcgtcggcga tgatcttgac
    12481 gaacccggtg gcatcaccca gcccccacgc cttgccgttg gcgctgaacg ggaacttcga
    12541 caccttcacg tcgtagccgc gctgtttcgc ctgctcttcg gtgtagccga acgagccgat
    12601 ctgcggctgg cagtaggtgg cgcggggaat catgtcgtag ttgatcgcca tcgtctcggc
    12661 gccggcgatg gtctcggcgg ccaccacacc ctgcgcctcc gcgacgtgcg ccagggcgag
    12721 cttcatggtg acgtcgccga tggcgtagac gcccggcacg ttggtgcgca
//
LOCUS       DAOSUG010000017        12244 bp    DNA     linear   ENV 10-JUN-2024
DEFINITION  MAG TPA_asm: Micropruina sp. isolate AMR_MDS_2195
            NODE_3002_length_12244_cov_3.669292, whole genome shotgun sequence.
ACCESSION   DAOSUG010000017 DAOSUG010000000
VERSION     DAOSUG010000017.1
DBLINK      BioProject: PRJNA925477
            BioSample: SAMN32864262
            Sequence Read Archive: SRR4236673
KEYWORDS    WGS; ENV; Metagenome Assembled Genome; MAG; Third Party Data; TPA;
            TPA:assembly.
SOURCE      Micropruina sp. (wastewater metagenome)
  ORGANISM  Micropruina sp.
            Bacteria; Bacillati; Actinomycetota; Actinomycetes;
            Propionibacteriales; Nocardioidaceae; Micropruina.
REFERENCE   1  (bases 1 to 12244)
  AUTHORS   Abdulkadir,N., Saraiva,J.P., Zhang,J., Stolte,S., Gillor,O.,
            Harms,H. and Rocha,U.
  TITLE     Genome-centric analyses of 165 metagenomes show that mobile genetic
            elements are crucial for the transmission of antimicrobial
            resistance genes to pathogens in activated sludge and wastewater
  JOURNAL   Microbiol Spectr 12 (3), e0291823 (2024)
   PUBMED   38289113
REFERENCE   2  (bases 1 to 12244)
  AUTHORS   Abdulkadir,N.
  TITLE     Direct Submission
  JOURNAL   Submitted (24-MAR-2023) Environmental Microbiology, Helmholtz
            Center for Environmental Research -UFZ, Permoserstr 15, Leipzig
            04318, Germany
COMMENT     The annotation was added by the NCBI Prokaryotic Genome Annotation
            Pipeline (PGAP). Information about PGAP can be found here:
            https://www.ncbi.nlm.nih.gov/genome/annotation_prok/
            
            ##Genome-Assembly-Data-START##
            Assembly Method        :: SPAdes v. 3.11.1
            Genome Representation  :: Full
            Expected Final Version :: Yes
            Genome Coverage        :: Not Applicable
            Sequencing Technology  :: Illumina MiSeq
            ##Genome-Assembly-Data-END##
            
            ##Genome-Annotation-Data-START##
            Annotation Provider               :: NCBI
            Annotation Date                   :: 05/04/2023 04:57:10
            Annotation Pipeline               :: NCBI Prokaryotic Genome
                                                 Annotation Pipeline (PGAP)
            Annotation Method                 :: Best-placed reference protein
                                                 set; GeneMarkS-2+
            Annotation Software revision      :: 6.5
            Features Annotated                :: Gene; CDS; rRNA; tRNA; ncRNA
            Genes (total)                     :: 2,776
            CDSs (total)                      :: 2,750
            Genes (coding)                    :: 2,714
            CDSs (with protein)               :: 2,714
            Genes (RNA)                       :: 26
            tRNAs                             :: 24
            ncRNAs                            :: 2
            Pseudo Genes (total)              :: 36
            CDSs (without protein)            :: 36
            Pseudo Genes (ambiguous residues) :: 0 of 36
            Pseudo Genes (frameshifted)       :: 2 of 36
            Pseudo Genes (incomplete)         :: 32 of 36
            Pseudo Genes (internal stop)      :: 2 of 36
            ##Genome-Annotation-Data-END##
FEATURES             Location/Qualifiers
     source          1..12244
                     /organism="Micropruina sp."
                     /mol_type="genomic DNA"
                     /submitter_seqid="NODE_3002_length_12244_cov_3.669292"
                     /isolate="AMR_MDS_2195"
                     /isolation_source="Activated sludge of municipal
                     wastewater treatment plants"
                     /db_xref="taxon:2737536"
                     /environmental_sample
                     /geo_loc_name="Canada"
                     /lat_lon="49.25 N 123.1 W"
                     /collection_date="2015-06-16"
                     /metagenome_source="wastewater metagenome"
                     /note="metagenomic"
     gene            198..407
                     /locus_tag="PLF56_01140"
     CDS             198..407
                     /locus_tag="PLF56_01140"
                     /inference="COORDINATES: protein motif:HMM:NF024139.2"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_function: GO:0003677 - DNA binding [Evidence IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="helix-turn-helix domain-containing protein"
                     /protein_id="HOQ52198.1"
                     /translation="MAQPAHHGNWGRPEPAWLSLQQAAVIYGVSTDTIRRRVASGDLP
                     AFRCGRRIIRVRAEDLSRLFREIPR"
     gene            complement(425..1612)
                     /locus_tag="PLF56_01145"
     CDS             complement(425..1612)
                     /locus_tag="PLF56_01145"
                     /inference="COORDINATES: protein motif:HMM:NF012798.2"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_function: GO:0003677 - DNA binding [Evidence IEA];
                     GO_process: GO:0006310 - DNA recombination [Evidence IEA];
                     GO_process: GO:0015074 - DNA integration [Evidence IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="tyrosine-type recombinase/integrase"
                     /protein_id="HOQ52199.1"
                     /translation="MSQRRGFGKLRSLPSGRYQASYVGPDLRRHNAPTTFQTKLDAEG
                     WLRDEYRMIERDEWTAPTVRSQVKYRPGSTLGDFAPEWLEAHKRSDGQPLKARTREHY
                     GNLLDRRVLPHLGQLPMRSITEERVSLWLDRELPQNTPVANAHAYALLGEILRAAARK
                     DPMIKPPIVAGASKARTKHKADPATLDELSLIINSMPERHRLAILLMAWCALRFGEVS
                     ELRRRDLVLSGGNPRIKVRRAVVLVGGQRVVTTPKSEAGDRDVTIPPHLLESIRQHLD
                     QFTEPGPDALLFTGERGGHLALTSFNGKPARRRRIMGRMVNESASGFCKAREVAGRPD
                     LHIHDLRHTGAVLAAQVGATLPELMERLGHSTPAAAMRYQHVARGRARAVADALSAMA
                     TGQ"
     gene            1842..2123
                     /locus_tag="PLF56_01150"
     CDS             1842..2123
                     /locus_tag="PLF56_01150"
                     /inference="COORDINATES: ab initio
                     prediction:GeneMarkS-2+"
                     /note="Derived by automated computational analysis using
                     gene prediction method: GeneMarkS-2+."
                     /codon_start=1
                     /transl_table=11
                     /product="hypothetical protein"
                     /protein_id="HOQ52200.1"
                     /translation="MNAQPWGPGQDAALLDAMRCYNADFAAWSANVQAGEIRLQGEER
                     AAQRAGYRAGRTGVDEPARRAATSAYNARELVAWAVGYGEGRATRELQP"
     gene            2222..3046
                     /locus_tag="PLF56_01155"
     CDS             2222..3046
                     /locus_tag="PLF56_01155"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_007077695.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_function: GO:0003723 - RNA binding [Evidence IEA];
                     GO_function: GO:0009982 - pseudouridine synthase activity
                     [Evidence IEA];
                     GO_process: GO:0001522 - pseudouridine synthesis [Evidence
                     IEA];
                     GO_process: GO:0009451 - RNA modification [Evidence IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="pseudouridine synthase"
                     /protein_id="HOQ52201.1"
                     /translation="MRGFLLDRLPADVPVAELLASGAFVDQHGRPFAAGEAYRHSTLI
                     WFHRAVLPEPPVPGEVRVLHSDDRIVVVDKPHFLATTPRGVHVRETVLVRLREQLDLP
                     DLAPAHRLDRLTAGVLLLTVQRRHRAAYSTLFQNRQVTKTYEALATFDPALDFPRRSL
                     DRIEKRRGSLQARVVEGEPNADTLIELVERRGGHARYRLTPRTGRTHQLRLQLAARGL
                     PIVGDPLYPRVLDVAPDDFTTPLQLVARRLGFRDPVDGTEREYTSRRLLDWPPSEE"
     gene            3083..3562
                     /locus_tag="PLF56_01160"
     CDS             3083..3562
                     /locus_tag="PLF56_01160"
                     /EC_number="2.1.1.207"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_016461622.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_function: GO:0003723 - RNA binding [Evidence IEA];
                     GO_function: GO:0008175 - tRNA methyltransferase activity
                     [Evidence IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="tRNA (cytidine(34)-2'-O)-methyltransferase"
                     /protein_id="HOQ52202.1"
                     /translation="MSATRPALRLLFCSPQIPGNTGAAMRLAAVTGAELHVARPIGFD
                     MDEAKLRRAGLDYRDQASTFVHDSLEHAYRALLPARVFAFTSHATVAHTDIAYRPGDV
                     LLFGPEPTGLPAEVLADQRVTERVRIPMRPGMRSLNLSNAAAIALYEAWRQLGFVGA"
     gene            complement(3587..4321)
                     /locus_tag="PLF56_01165"
     CDS             complement(3587..4321)
                     /locus_tag="PLF56_01165"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_017932819.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="DUF72 domain-containing protein"
                     /protein_id="HOQ52203.1"
                     /translation="MNGSAWIGTSGWSYDHWDGVLYPPGTPARDRLAHYVAEFRTVEL
                     NASFYRWPKTATFASWRRRLPDGFRLTVKAPRGLTHSKRLLHPDQWCRWIERDWHALF
                     DRRGVLLVQLPPGMGRDDARLDYFLSRLPWWVPVAVEFRHDSWVCDEVFALLERHGAA
                     YVVMSGAGLPCVLRATAPFAYVRLHGPDSDWLYGGSYSEADLHWWAERVREWQAQGRD
                     VYAYFNNDGGGNAVRNARTLRAILGQ"
     gene            4378..4932
                     /locus_tag="PLF56_01170"
     CDS             4378..4932
                     /locus_tag="PLF56_01170"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_020385052.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_function: GO:0003677 - DNA binding [Evidence IEA];
                     GO_function: GO:0003700 - DNA-binding transcription factor
                     activity [Evidence IEA];
                     GO_function: GO:0016987 - sigma factor activity [Evidence
                     IEA];
                     GO_process: GO:0006352 - DNA-templated transcription
                     initiation [Evidence IEA];
                     GO_process: GO:0006355 - regulation of DNA-templated
                     transcription [Evidence IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="sigma-70 family RNA polymerase sigma factor"
                     /protein_id="HOQ52204.1"
                     /translation="MAERDETTALALAAGQGDRAALSDLIRRTQGDVWLFLARSVGPE
                     QADDLTQETFLRMLGSLPGFEARASARTWLLSIARRVAVDRVRHDQARPRLVDADWQH
                     EADRRDVGDDPSSAVYAAHLLDGLDADRRDALVLTQLMGFSYAECAEICGCAVGTIRS
                     RVARARAELVAALDAEQRRASALR"
     gene            complement(4946..5800)
                     /locus_tag="PLF56_01175"
     CDS             complement(4946..5800)
                     /locus_tag="PLF56_01175"
                     /inference="COORDINATES: protein motif:HMM:NF028506.2"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="DUF5134 domain-containing protein"
                     /protein_id="HOQ52205.1"
                     /translation="MFSLADTPVKFIGLLILFAWCTLWCGYELTRPQNGRQRVSNVLH
                     LAMAVVMLLMVAAPTWTLLTAVVPDVWLTAGFAASTVWFVVVAGRGFATGDRSGGRHG
                     LGHAAMFGAMTWHLAAMAAMSVAMTTGMSGHGGMADHGGMSDHGGMGGAMDMGAWMAE
                     QSRPGGLLWSFALIGVPFMVYLLASSLYSAWLATRPSVTPGAAETRSASSEPRVSTDP
                     RVSASAVTLATRSVVAANPACHEVRPQGSLTYRLAHSADFAMNAGMFWMSTGLLVPLL
                     PFFAALSF"
     gene            complement(6005..6331)
                     /locus_tag="PLF56_01180"
     CDS             complement(6005..6331)
                     /locus_tag="PLF56_01180"
                     /inference="COORDINATES: protein motif:HMM:NF024881.2"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="zf-HC2 domain-containing protein"
                     /protein_id="HOQ52206.1"
                     /translation="MNRLSHEQCRDAVSALLDGEEPPVGDEAVMAHLAACAQCSAWLE
                     AATRVNAGVRNLPVVSSTLGERVVDRVDVHLCACATGGTCRCGNCQCGPSCTCHSGSA
                     SQRTAR"
     gene            complement(6381..8651)
                     /locus_tag="PLF56_01185"
     CDS             complement(6381..8651)
                     /locus_tag="PLF56_01185"
                     /EC_number="7.2.2.-"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_002546520.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_component: GO:0016020 - membrane [Evidence IEA];
                     GO_function: GO:0005524 - ATP binding [Evidence IEA];
                     GO_function: GO:0019829 - ATPase-coupled monoatomic cation
                     transmembrane transporter activity [Evidence IEA];
                     GO_process: GO:0006812 - monoatomic cation transport
                     [Evidence IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="heavy metal translocating P-type ATPase"
                     /protein_id="HOQ52207.1"
                     /translation="MTTETAPAPQPDALGERVELDVAGMTCAACASRIERKLNKLDGA
                     RATVNYATERAVVLGWPPQRADELIETVRAAGYDASEVRTDDDGQADARADRVRMLRN
                     RLIVAAVLTVPLGDAALVLALAPQLRFPGWEWTLIIGALPVVFWCAWPFHKAAWRNLR
                     HGTTSMDTLVSLGVLASFTWSVVATIVRVPGEPSYWLGYGLAPDGADSLYLEAAAAVT
                     TFLLAGRYVEARSKRSARGVLTAIGRLAPSTVRVLRDGVEHEVPIGSLAVGERFVVRP
                     GERVATDGVVVAGASAIDTAAMTGEAEPVEVGIGGRVLAGTTNTTGALVVEAQRIGAH
                     TQLAQLAVLAEQAQARKASVQSLVDRVVAVFVPVVLVISALTLTGWLIAGGGLRHAFG
                     AAMSVLIIACPCALGLATPTALLVGVGRGGQLGILIKGPDALEASGRIDTVVLDKTGT
                     LTTGRMTLVEAVRFDGTSADDLLRLAAAVETDSEHPIAAAVVAGAAARGLATARPENV
                     RALPGGGVQGEVSEAGDTRRVLIGTPELLAERVGEVTPEAIAAVETAAAQGRSAVLVA
                     VDDVVVGYLVLADTIKPSAAAAVRRLGGLGLRTVLLSGDRRAVAEQVGAAVGVDEVIA
                     EVLPSAKAEAIERLQAQGRRVAMVGDGINDAGALATANLGMAVVNGTDVALKSADIIL
                     VRRSLDAVPDAIQLSRRTLGTIRGNLIWAFGYNVAAIPLAAAGLLNPLIAGLAMSLSS
                     VFVVGNSMRLRRFTPR"
     gene            complement(8641..10638)
                     /locus_tag="PLF56_01190"
     CDS             complement(8641..10638)
                     /locus_tag="PLF56_01190"
                     /inference="COORDINATES: protein motif:HMM:NF021214.2"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="cytochrome c oxidase assembly protein"
                     /protein_id="HOQ52208.1"
                     /translation="MSQSRGGLRLGSVTLVVLGLAVLAAFVQTAAYLGLLVAQPALPG
                     RLNAEPLTGAVSLFAGLAGRLAGAAALGLLAALTAFSPRGSSADPADPARAALTRWTL
                     RAAQVWLAAALLMTFANPTFVLGLPLQLAFVPSSWWTFLTTTPSALAWLVSALAALAT
                     VLVTLRVRSPGPLVGLWLLGAGATVFVAVTGNVTVGLNHDWATDAMALACLAGVLISS
                     GAVAVVATAAAGVDPRAGLARYVRAAPLVAVMALGYAVVAWQQLAGVSPFEVAFGLPV
                     VIGFAALGLLLASAVVRAFVGSVRVRLGSVLIDLVLSVIAATSLNAAEHLPPPRFTVP
                     QSTQINYLGYEVLDPPTAARVAGWGRPNLLWVVLVVSGLSLYLIGVLRARRLGRDWPL
                     TRGIFLLAGLGLTGYLAVSGLWEYSTAAFSWHMLVHMTVNMLIPLLCVLSAPLALVRA
                     ASRRRSDDELPGIADLLDDLARTRLARVLTSAPVLWAGYVFSLFLVYFSPLFPWLMRY
                     HWGHQAMLLFFMGVGFAFFSLLLAPGEQAGRLPYVLRFALLISVMPFHAMFAVGIMSS
                     KGLIGEQFYRTLAMPWVPDLLADQNIAGQITWFTGEVPAFIAVLMLAAQWFRSDSRAA
                     AVADRRADELGDSELGAYNDMLAELAARDASGRARWRNDDD"
     gene            10816..11424
                     /locus_tag="PLF56_01195"
     CDS             10816..11424
                     /locus_tag="PLF56_01195"
                     /inference="COORDINATES: protein motif:HMM:NF024587.2"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_function: GO:0005524 - ATP binding [Evidence IEA];
                     GO_function: GO:0016301 - kinase activity [Evidence IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="cytidylate kinase-like family protein"
                     /protein_id="HOQ52209.1"
                     /translation="MTATAVHQSVVINREFGSGGREIGRLISQQLGADFFDSRILQDA
                     AASKGLPANQLAAFDERLVAGAYLNLGITGFSDPELTTLPYRMFSAFAEVITEAAKRA
                     PAVFIGRCADVILHEAGLPVRSVFVYSTDAAAKVARAVQVDHVPPKEAERHIARMDKA
                     RSRYQAFFTGTRFGETHEYDLCLNSGQISPQACAAAVIATLT"
     gene            complement(11453..11683)
                     /locus_tag="PLF56_01200"
     CDS             complement(11453..11683)
                     /locus_tag="PLF56_01200"
                     /inference="COORDINATES: ab initio
                     prediction:GeneMarkS-2+"
                     /note="Derived by automated computational analysis using
                     gene prediction method: GeneMarkS-2+."
                     /codon_start=1
                     /transl_table=11
                     /product="hypothetical protein"
                     /protein_id="HOQ52210.1"
                     /translation="MSQENQNPPRAVGNPGPADGSAEEFDQPRSIGNPGSTPDPRDEY
                     DRPKAVGNPGPADDPDEEFEQPRSIGNPGPED"
     gene            complement(11799..>12244)
                     /locus_tag="PLF56_01205"
     CDS             complement(11799..>12244)
                     /locus_tag="PLF56_01205"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_013861885.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=3
                     /transl_table=11
                     /product="zinc-binding dehydrogenase"
                     /protein_id="HOQ52211.1"
                     /translation="IAMSRHESRAALAREFGATHVVAERDEAGSAAVKALTDGIGADC
                     VLECVGTEGARLQAVGSVRDGGNIGLVGVPHGDLPVNELFWRNVGIKGGTANVRAYLP
                     RLLELVLARSIDPGRVFDLELPLSQVAQAYAAMDERRAIKVLLRP"
BASE COUNT         1988 a         4456 c         4096 g         1704 t
ORIGIN      
        1 ccgacgacca ccctgccgac gccctcagct accgcatcac cgcactgatc aagaagcacc
       61 gccaggccgc ggccgaaacc tgggaaacga tcacccccac agcccccagg ccctaccccg
      121 aacactcacg accccacgac cacgaccgcg gccgcggcat cagcatctga acccatgcga
      181 atcgagagga caccgccatg gcacagcccg cccaccacgg taactgggga cgacccgaac
      241 ccgcatggct ctcgctccag caagccgcgg tcatctatgg cgtcagcacc gatacgatcc
      301 ggcgacgggt ggcctcgggg gatcttcccg ccttccgatg cggtcgccgg attatccgcg
      361 tgcgcgcaga agacctgtcg cgcctgtttc gcgagatccc cagatgaccg ggccggtcgc
      421 ccagctattg cccggtggcc atggcggaaa gcgcgtcggc gacggcccgc gcccttccgc
      481 gagcgacgtg ctggtagcgc atcgccgctg ccggggtcga gtgacccagt cgctccatga
      541 gttcgggcag tgtcgcgccg acctgggccg ccaataccgc cccggtgtgc ctgaggtcgt
      601 ggatgtggag atcgggtcgc ccggctacct cacgggcctt acagaacccg gacgccgact
      661 cgttgaccat gcgacccatg atgcgccggc gccgggcggg cttgccgttg aagctcgtga
      721 gtgcgagatg cccaccacgt tcgccggtaa acagcaaggc gtccggtccg ggttcggtga
      781 actggtccag atgctgccga atggactcca gcaggtgcgg cggaatcgtc acgtcccggt
      841 ctcccgcctc gctctttggc gtggtgacca cgcgctgtcc gccgaccagc acgaccgctc
      901 ggcgaacctt gatccgggga ttgccgccgc tcaggacgag atcccgtcga cgaagctcgc
      961 tcacctcgcc gaaccgcagc gcgcaccagg ccatcagtag gatcgcgagc cgatggcgct
     1021 cgggcatgct gttgatgatc aagctcagtt cgtcgagtgt cgccgggtcg gccttgtgct
     1081 tcgtcctcgc cttggacgcg ccagcgacta ttggaggctt gatcattgga tccttgcgcg
     1141 ccgcggctcg gaggatctcg cccagcaggg cataggcgtg ggcgttggcc accggcgtgt
     1201 tctgtggaag ttcgcgatcg agccagaggc ttactcgctc ttccgtgatc gatcgcatcg
     1261 gcagttgacc cagatgcggc aggacacgtc tgtccagaag gttcccgtag tgctcgcgag
     1321 ttcgtgcctt cagcggttgc ccgtcactgc gtttgtgggc ctccagccac tcgggtgcga
     1381 aatcgcccag tgtggagccg ggccggtact tgacctgcga tcggaccgtc ggcgcggtcc
     1441 actcgtcccg ctcgatcatg cggtactcat cgcgcagcca gccctcggcg tcgagcttgg
     1501 tctggaaggt cgtgggcgcg ttgtggcgcc gaaggtcggg gccgacgtag ctagcctgat
     1561 acctgcctga cggcagactg cgaagcttgc cgaaaccgcg ccgctgcgac atgtgggcat
     1621 ccttccaacg acccggtcgt gcaccatacg tgcaccatcg tagccgaatt gagccccttt
     1681 ctgcctactt gtgcaatcgt cgactctccc catctggaca ttgatttgcc tagtcagaga
     1741 cctatggcaa gcacagttcc agacggggaa gtcacacccc gattctgatt cgagtccagg
     1801 cgggggagcc accttcaact cgggcagggc agactgaggc gatgaacgcg cagccatggg
     1861 ggcccggcca ggacgccgcg ctacttgacg ccatgcggtg ctacaacgct gacttcgctg
     1921 catggtccgc caacgtccaa gcgggtgaga tcaggctgca gggcgaggag cgggcggcgc
     1981 agcgcgccgg gtaccgggcc ggcaggaccg gcgtcgacga gccggcgcgc cgcgctgcca
     2041 cctcggccta caacgcccgc gaactcgtcg cctgggcagt cggctacggc gaggggcgag
     2101 cgacccgcga attgcagccg tagtggcgcc acgctcaccg ctgccgcaac gccatgggct
     2161 ggacgcggcc tggatgcgga cccccgaccg tagccccggc gtccagccgc cctggacgac
     2221 catgcgcggc tttctgctcg accggctgcc ggcggacgtc ccggtggcgg agttgctggc
     2281 ctccggcgcc ttcgtcgacc agcacggacg tcccttcgct gccggtgagg cctatcggca
     2341 cagcaccctg atctggtttc accgggccgt gctgccggaa ccacccgtgc cgggcgaggt
     2401 gcgcgtgctc cactccgacg accgcatcgt cgtcgtcgac aagccacact ttctggccac
     2461 cacgccgcgc ggggtgcacg tgcgcgagac cgtgctggtg cggctgcggg agcaactgga
     2521 cctgcccgac ctcgcgcccg cgcaccggct cgaccggctg actgccggag tgctgctgct
     2581 gaccgtccag cgcaggcacc gcgccgcgta ttcgaccctg tttcagaacc ggcaggtgac
     2641 caagacctat gaggcgctgg caacgttcga tcctgcgctg gactttccgc gacggtcgct
     2701 cgatcgcatc gagaaacgac gcggcagcct gcaggcccgg gtggtcgagg gcgagcccaa
     2761 cgccgacacc ctgatcgaac tcgtcgagcg gcgaggaggc cacgcccgct accggctcac
     2821 tccccgcacc ggccgcaccc accaactgcg cctccagctc gccgctcgcg gcctgccgat
     2881 cgtcggcgac ccgctctacc ctcgtgtgct ggacgtcgcc cccgacgact tcaccacccc
     2941 gctgcagctg gtcgcgcgtc ggctcggctt ccgcgacccg gtcgacggca ccgagcgtga
     3001 gtacaccagc cgacgtttgc tcgactggcc gccctccgaa gagtgaccgg ggcaacgtcc
     3061 ccggcgtgcc acagtgtcgg acatgagtgc caccaggccc gctttgcggc tgctgttctg
     3121 ttccccgcag attcccggca ataccggagc ggccatgcgg ctcgcggcgg tgaccggcgc
     3181 ggaactgcac gtggcgcgtc cgatcggttt cgacatggac gaagcgaaac tgcgtcgggc
     3241 cggcctcgac taccgcgacc aggcgtcgac cttcgtgcac gactcactcg agcacgccta
     3301 ccgggcactg ctgccggctc gggtgttcgc gttcacctca cacgcaacgg tcgcgcacac
     3361 cgacatcgcc taccggcccg gcgacgtgtt gctgttcggc cccgagccca ccgggttgcc
     3421 ggccgaggtg ctggccgacc agcgggtgac cgaacgggtg cgcattccga tgcggcccgg
     3481 catgcgctcg ctcaacctga gcaacgccgc ggcgatcgcc ctgtacgagg cctggcgcca
     3541 actggggttc gtcggggcct gagctggtct gactcgtcga cctcggctat tgcccgagaa
     3601 tcgcgcgcag ggtgcgggcg ttgcgtaccg cgttgccgcc gccgtcgttg ttgaagtagg
     3661 cgtagacgtc tcggccctgc gcctgccact cgcggacgcg ctcagcccac cagtgcaggt
     3721 cggcctcgga gtacgaaccg ccgtacaacc agtcggagtc gggtccgtgc agccgcacgt
     3781 aggcgaacgg cgcggtggcc cgcaggacgc acggcagccc ggcacccgac atcaccacgt
     3841 aggccgcacc gtgccgttcc agcagggcga acacctcgtc gcacacccac gagtcgtgcc
     3901 gaaactccac cgccaccggc acccaccagg gcagccgcga caagaagtag tccagtcgcg
     3961 catcgtcccg gcccatgccg ggcggcaact gcaccagcag cacaccccgg cggtcgaaca
     4021 gggcatgcca gtcgcgttcg atccagcggc accactggtc ggggtgcagc aggcgcttgc
     4081 tgtgggtgag cccgcgcggt gctttcacgg tcagccgaaa gccgtccggc agccgccgcc
     4141 gccacgaggc gaaggtcgcg gtcttcggcc agcggtagaa ggaggcgttc aactcgacgg
     4201 tgcgaaactc ggccacataa tgcgcaagcc ggtcgcgcgc cggggtgccc ggcgggtaca
     4261 gcacgccgtc ccagtggtcg taactccagc ccgacgtgcc gatccacgcg gagccgttca
     4321 tgcgtcgagc ctaggcacga cgcctcgtgg cgggccgcac cggttagggt gaatgcgatg
     4381 gctgagcgcg acgagacgac cgccctggcc ctggccgccg gccagggtga ccgggctgcc
     4441 ctctccgacc tgatccggcg cacccagggc gacgtctggc tgttcctggc ccgctcagtg
     4501 ggccccgaac aggccgacga cctcacccag gaaacgttcc tgcgcatgct gggttcgttg
     4561 cccggcttcg aggcccgcgc cagtgcccgc acctggctgc tgtccatcgc ccgtcgggtc
     4621 gccgtcgacc gggtccggca cgatcaggcc cggccgcggc tggtcgatgc ggattggcag
     4681 cacgaggcgg atcgccgtga cgtcggtgac gatccgtcgt ccgccgttta cgcggcgcac
     4741 ctgctcgacg ggctggacgc ggatcgtcgc gacgccctgg tgctcaccca gttgatgggt
     4801 ttcagctatg ccgaatgcgc cgagatctgc gggtgcgcgg tgggtacgat ccgctcgcgg
     4861 gtggcgcggg cgcgggctga gctggtcgcg gcgctcgatg ccgagcaacg ccgggcgtcc
     4921 gcgctgcgtt gacgcgttgg tgcgctcaga aggaaagcgc ggcgaagaac ggcagcagtg
     4981 gcaccagcag gccggtgctc atccagaaca tgcccgcatt catcgcgaaa tcggcggagt
     5041 gggccaggcg gtaggtgagg ctgccctgcg gacgcacctc gtgacaggcg gggttggcgg
     5101 ccacgaccga ccgggtcgcc agggtgaccg cgctcgccga gaccctcggg tcggtcgaga
     5161 cgcggggttc ggacgaagcg gagcgggtct ccgcagcgcc tggcgtcacg ctcggacgcg
     5221 tcgccagcca tgccgagtac agcgacgagg cgagcaggta gaccatgaac ggtacgccga
     5281 tcagcgcgaa gctccacagc agcccgcccg gccgactctg ctcggccatc caggcgccca
     5341 tatccatcgc accgcccatg ccgccgtggt cgctcatacc gccatggtcg gccatgccgc
     5401 cgtgcccgct catgccggtg gtcatcgcca ccgacatggc cgccatcgcg gccaggtgcc
     5461 aggtcatggc gccgaacatc gccgcatggc ccagtccgtg ccggccgccg gagcgatcac
     5521 cggtggcgaa acccctgccc gcgacaacga cgaaccacac cgtggacgca gcgaaaccgg
     5581 ccgtcagcca gacgtccggc acgacggctg tcagcagggt ccaggtcggc gcggccacca
     5641 tgagcagcat caccaccgcc atcgctaggt gcagcacgtt cgacacccgc tgccgtccgt
     5701 tctgaggccg ggtcagctca tagccgcacc acagcgtgca ccaggcgaac aggatcagca
     5761 gtccgatgaa cttcaccggg gtgtcggcaa gactgaacat gacgtcactt tcgtcgcagg
     5821 gggccttcca ccgattagtc ggacgcagcg tccagatagt tcccgaactc gctcagtagg
     5881 gcacccaccg gatcgcaagc agtaccgaat ccacactcga tgtcgacttg aacgattcaa
     5941 tcgcaacatt ctcgaatcga gcggtggatt tggtatgtca gcgggtgatg attttgcgtt
     6001 cgggtcagcg cgcggtgcgc tgagatgccg agccgctgtg gcaggtgcag gacggtccgc
     6061 actggcagtt gccgcatcgg cacgtgccgc cggtggcgca cgcacacagg tgcacgtcga
     6121 cccgatcgac cacccgttct ccaagcgtcg acgaaaccac cggcaggttg cgcacgcctg
     6181 cgttcacccg ggtcgcggcc tccagccagg cggagcactg cgcgcacgcg gcgaggtggg
     6241 ccatcacggc ctcgtcaccc accggcggct cttcaccatc gagtagcgcc gagaccgcgt
     6301 cacggcactg ctcatgggac agccggttca tcgcgccagc ataggcccgg acgaaccgcc
     6361 caccggccgc cggcgactga ctaccggggg gtgaaccgcc gcagccgcat cgagttgccc
     6421 accacgaaca cgctcgacag cgacatcgcc aacccggcga tcaacgggtt gagcagcccg
     6481 gcggcagcca ggggaatggc ggccacgttg tagccgaagg cccagatcag gttgccgcga
     6541 atggtgccca gggtgcgccg cgacagctgg atcgcgtccg gcacggcgtc caggctgcgc
     6601 cgcaccagga tgatgtcggc cgacttcagt gccacgtcgg tgccgttcac caccgccatg
     6661 cccaggttgg ccgtggcgag ggcgcccgcg tcgttgatgc cgtcacccac catcgccacc
     6721 cgtcgtccct gcgcctgcag ccgctcgatc gcctcggcct tggcgctcgg caacacttcg
     6781 gcgatcacct cgtccacgcc gaccgcggcg ccgacctgct cggccaccgc ccgccggtcg
     6841 ccgctgagca gcaccgtgcg caggccgagg ccacccagtc gccggacggc cgccgcagcg
     6901 gacggcttga tagtgtcggc cagcaccaga tagcccacca cgacgtcgtc caccgccacc
     6961 agcaccgcgc tgcggccctg cgccgcggca gtctcgacgg cagcgatcgc ctcgggcgtc
     7021 acctcgccga cccgctcggc cagcaactcg ggcgtgccaa tcagcacccg ccgcgtgtcg
     7081 ccggcctcgc tcacctcgcc ctgcaccccg ccacccggca gggcccggac gttctcgggt
     7141 cgcgccgtcg ccagcccccg cgcagccgca cctgcgacca ccgccgccgc gatcggatgc
     7201 tccgagtcgg tctcgaccgc cgcggccagt cgcagcaggt cgtcggcact ggttccgtca
     7261 aagcggacgg cctcgaccag cgtcatgcgt ccggtggtca gggtgccggt cttgtcgagc
     7321 accaccgtgt cgatgcgccc cgaggcctcc agcgcgtccg ggcccttgat cagaatgccg
     7381 agctgaccgc cccgcccgac gcccaccagc agggcggtcg gggtggccag gccgagggca
     7441 caggggcagg cgatgatcag caccgacatg gcggccccga acgcgtgccg cagcccgccg
     7501 ccggcgatca gccagccggt cagggtcagc gccgagatca ccagcacgac cggcacgaac
     7561 accgccacga cgcgatcgac cagcgactgc acggacgcct tgcgggcctg agcctgttcg
     7621 gccagtactg ccagttgcgc gagctgggtg tgcgccccga tgcgctgcgc ctcgaccacc
     7681 agggcgccgg tggtgttcgt ggtgccggcc agcacccggc ctccgatgcc cacctcgacc
     7741 ggttcggcct caccggtcat cgcggcggtg tcgatggccg acgcacccgc gaccaccaca
     7801 ccgtcggtgg cgacccgctc gcccggacgg accacgaacc gctcgccgac cgccagcgaa
     7861 ccgatcggca cctcgtgctc gaccccgtcg cgcagcaccc ggacggtgct gggcgccagc
     7921 cgtccgatgg cggtcagcac gccacgggcc gagcgtttcg accgggcctc gacgtagcgt
     7981 ccggcgagta ggaacgtggt gacggcggcg gcggcctcga ggtagaggct gtcggcaccg
     8041 tcgggggcca acccgtagcc gagccagtac gagggttcgc ccggcacgcg cacgatggtg
     8101 gccacgaccg accaggtgaa cgatgccaac acccccagcg agaccagggt gtccatgctg
     8161 gtggtgccgt gccgaaggtt gcgccacgcc gccttgtgga acggccaggc gcaccagaac
     8221 accaccggca gtgcgccgat gatcagcgtc cattcccaac cggggaagcg cagttgcggt
     8281 gccagcgcga gcaccagagc ggcatcgccc aagggcacgg tgagcacggc ggcgacgatc
     8341 aagcggttgc gcagcatccg gacgcggtcg gcgcgtgcgt cggcctgccc gtcatcgtcg
     8401 gtacgcacct cgctcgcgtc atagcccgcc gcccggactg tctcgatcag ctcgtcggcc
     8461 cgctgcggcg gccagccgag gaccacggcc cgctcggtgg cgtagttgac ggtggcgcgt
     8521 gccccgtcca gcttgttcag cttgcgctcg atgcggctcg cgcaggccgc gcaggtcatg
     8581 ccggcgacgt cgagttcgac gcgttcgccc agggcgtccg gctggggggc cggtgcggtc
     8641 tcagtcgtca tcgttgcgcc acctcgcacg tccggacgcg tcgcgcgcgg cgagttcggc
     8701 cagcatgtcg ttgtaggcgc ccagttcgga gtcacccagc tcatcggccc ggcggtccgc
     8761 gacggcggcg gcgcggctgt cggagcggaa ccactgtgcg gccagcatca gcacggcgat
     8821 gaacgccggc acctcgccgg tgaaccaggt gatctgcccg gcgatgttct ggtcggccag
     8881 taggtcgggc acccacggca tggccagcgt gcggtagaac tgctcgccga tcaggccctt
     8941 gctgctcatg atgccgaccg cgaacatggc gtgaaacggc atcacgctga tcaacagcgc
     9001 gaagcgcagc acgtacggca gccggccggc ctgctcgccc ggtgccagca gcaggctgaa
     9061 gaaggcgaaa ccgacgccca tgaagaacag cagcatcgcc tgatgacccc agtgatagcg
     9121 catcagccag gggaacagcg ggctgaagta caccaggaac agcgagaaca cgtagccggc
     9181 ccacagcacc ggggcactgg tcagcacccg cgcgagccgg gtccgggcga ggtcgtccag
     9241 caggtcggcg atccctggca gttcgtcgtc gctgcgtcgc ctggacgccg cccgcaccag
     9301 ggccagcggt gcgctcagca cgcaaagcag cgggatcagc atgttgaccg tcatgtgcac
     9361 gagcatgtgc cagctgaacg ccgccgtgct gtactcccac aggccgctga ccgccagata
     9421 gccggtcagg ccgagcccgg ccaacaggaa gatgccccgg gtgagcggcc agtcgcggcc
     9481 cagccgccgg gcccgcagca cgccgatcag atacaggctc agcccgctga cgacgagcac
     9541 cacccagagc aggttcggcc ggccccaacc ggccacgcgg gccgcggtgg gcgggtcgag
     9601 cacctcataa cccaggtagt tgatctgcgt ggactgcggc acggtgaagc gcggcggcgg
     9661 caggtgttcg gcggcattca ggctggtcgc ggcgatcacc gacagcacca ggtcgatcag
     9721 cacgctaccc agccgcaccc gtaccgagcc gacgaatgcg cgcaccaccg ccgaggccag
     9781 cagcagcccc agggccgcga aaccgatcac caccggcaac ccgaaggcga cctcgaacgg
     9841 cgacaccccg gccaactgct gccaggccac caccgcataa ccaagcgcca tcaccgccac
     9901 cagcggggcc gcgcggacgt agcgcgcaag accggcgcgc ggatcgacac ccgccgccgc
     9961 tgtcgccacc acggccaccg caccggacga gatcagcacc ccggcgaggc acgcaagcgc
    10021 catcgcgtcc gtcgcccagt cgtggttgag gccgacggtg acgttgccgg tgacggccac
    10081 gaacaccgtc gccccggcgc ccagcagcca gagtccgacc agcgggccgg gggagcgcac
    10141 ccgcagcgtg accagcaccg tggcgagcgc ggcgagcgcc gacaccagcc acgccagcgc
    10201 gctgggagtg gtggtcagaa acgtccacca cgacgacggt acgaaggcca gctgcaacgg
    10261 aaggccgagc acgaaggtgg ggttcgcgaa ggtcatcagc agggcggcgg ccagccacac
    10321 ctgcgcagcg cgcagcgtcc agcgggtgag tgcggcgcga gccggatcag cggggtcggc
    10381 ggacgatccg cggggcgaaa aggcggtcag tgcggccaac aggcccaggg ctgcggcacc
    10441 ggccagccgc ccggccagcc cggcgaacag cgacaccgcg ccggtcaacg gctcggcgtt
    10501 cagccggccg ggcagggcgg gttgtgcgac cagcaggccc aggtacgcgg cggtctggac
    10561 gaacgccgcc aacacggcca ggccgagcac gaccagtgtc accgacccga gccgaaggcc
    10621 gccccgcgac tgcgacatcg accactcacc ccggctcgta acggacaacg aagacgctgt
    10681 gagtctaggg cgtgtctcgc cacggacgcc ccaacccacg ctgtcgtgca agacccctgc
    10741 ggcgcggccg cgccgggcgt atcgtcgaag agtagccaga accactgacc acgagatggc
    10801 aagggggccc aggcgatgac cgccacagca gtacatcagt ccgtcgtgat caaccgcgag
    10861 ttcggtagcg gaggacgcga gatcggtcgg ctgatcagcc agcagctcgg cgccgacttc
    10921 ttcgacagtc gcattctgca ggacgcggcc gcgtccaagg ggctgcctgc caaccagttg
    10981 gcggcattcg acgagcggct ggttgccggt gcctacctga acctgggcat caccgggttc
    11041 agcgatcccg agctgaccac gcttccgtac cgcatgttct cggccttcgc cgaggtgatc
    11101 accgaggccg ccaagagggc tcccgcggtc ttcatcggac gctgtgccga cgtgattctg
    11161 cacgaggcgg gcctgccggt gcgttcggtg ttcgtgtaca gcacggacgc ggcggccaag
    11221 gtggcccggg ccgttcaggt cgaccatgtg ccgcccaaag aggccgagcg gcacatcgcc
    11281 cgcatggaca aggcccgcag ccgctaccag gcgttcttca ccggcacccg attcggcgag
    11341 acccacgaat acgacctgtg cctgaacagc ggccagatca gcccccaggc ctgcgccgcc
    11401 gcagtcatcg caacgctcac ctgagccggg cctcgcgcgg cccagcgcac ttctagtcct
    11461 cgggcccggg gttgccgatc gaacggggct gctcaaactc ttcgtcgggg tcgtcggccg
    11521 ggccgggatt gccgaccgcc ttcggacggt cgtactcgtc gcgcgggtcg ggggtcgagc
    11581 cggggttgcc gatcgacctg ggctggtcga actcctcagc cgagccgtcc gctggcccag
    11641 ggttgccgac cgcgcgcggc gggttctgat tctcttgcga cactgcattc ctccaagcgt
    11701 gattcgccgg gcgttcccgt ccggcgaagg cggtgctcca agcagactac tgatcgaccc
    11761 cgacccgcaa ccccgcgccg gacggctgca cgaatgactc aggggcgcag cagaaccttg
    11821 atcgcgcggc gctcatccat ggctgcgtag gcctgcgcca cctgcgacag cggcaactcg
    11881 aggtcgaaga cccgtccggg atcgatgctg cgggccagca cgagctcgag caggcggggc
    11941 agataggccc gcacgttggc ggtgcccccc ttgatgccga cgttgcgcca gaacagttcg
    12001 ttgaccggca gatcgccgtg cggcacgccg accaggccga tgttgccccc gtcgcggacg
    12061 ctgcccaccg cctgcagccg ggcgccctcg gtgccgacgc actcgagcac gcagtcggcc
    12121 ccgatgccgt cggtcagcgc cttgaccgcg gcgctgcccg cctcgtcgcg ctcggccacc
    12181 acatgcgtgg cgccgaactc gcgcgccagc gccgcgcgcg actcgtgccg gctcatcgcg
    12241 atca
//
LOCUS       DAOSUG010000018        11716 bp    DNA     linear   ENV 10-JUN-2024
DEFINITION  MAG TPA_asm: Micropruina sp. isolate AMR_MDS_2195
            NODE_3118_length_11716_cov_4.590172, whole genome shotgun sequence.
ACCESSION   DAOSUG010000018 DAOSUG010000000
VERSION     DAOSUG010000018.1
DBLINK      BioProject: PRJNA925477
            BioSample: SAMN32864262
            Sequence Read Archive: SRR4236673
KEYWORDS    WGS; ENV; Metagenome Assembled Genome; MAG; Third Party Data; TPA;
            TPA:assembly.
SOURCE      Micropruina sp. (wastewater metagenome)
  ORGANISM  Micropruina sp.
            Bacteria; Bacillati; Actinomycetota; Actinomycetes;
            Propionibacteriales; Nocardioidaceae; Micropruina.
REFERENCE   1  (bases 1 to 11716)
  AUTHORS   Abdulkadir,N., Saraiva,J.P., Zhang,J., Stolte,S., Gillor,O.,
            Harms,H. and Rocha,U.
  TITLE     Genome-centric analyses of 165 metagenomes show that mobile genetic
            elements are crucial for the transmission of antimicrobial
            resistance genes to pathogens in activated sludge and wastewater
  JOURNAL   Microbiol Spectr 12 (3), e0291823 (2024)
   PUBMED   38289113
REFERENCE   2  (bases 1 to 11716)
  AUTHORS   Abdulkadir,N.
  TITLE     Direct Submission
  JOURNAL   Submitted (24-MAR-2023) Environmental Microbiology, Helmholtz
            Center for Environmental Research -UFZ, Permoserstr 15, Leipzig
            04318, Germany
COMMENT     The annotation was added by the NCBI Prokaryotic Genome Annotation
            Pipeline (PGAP). Information about PGAP can be found here:
            https://www.ncbi.nlm.nih.gov/genome/annotation_prok/
            
            ##Genome-Assembly-Data-START##
            Assembly Method        :: SPAdes v. 3.11.1
            Genome Representation  :: Full
            Expected Final Version :: Yes
            Genome Coverage        :: Not Applicable
            Sequencing Technology  :: Illumina MiSeq
            ##Genome-Assembly-Data-END##
            
            ##Genome-Annotation-Data-START##
            Annotation Provider               :: NCBI
            Annotation Date                   :: 05/04/2023 04:57:10
            Annotation Pipeline               :: NCBI Prokaryotic Genome
                                                 Annotation Pipeline (PGAP)
            Annotation Method                 :: Best-placed reference protein
                                                 set; GeneMarkS-2+
            Annotation Software revision      :: 6.5
            Features Annotated                :: Gene; CDS; rRNA; tRNA; ncRNA
            Genes (total)                     :: 2,776
            CDSs (total)                      :: 2,750
            Genes (coding)                    :: 2,714
            CDSs (with protein)               :: 2,714
            Genes (RNA)                       :: 26
            tRNAs                             :: 24
            ncRNAs                            :: 2
            Pseudo Genes (total)              :: 36
            CDSs (without protein)            :: 36
            Pseudo Genes (ambiguous residues) :: 0 of 36
            Pseudo Genes (frameshifted)       :: 2 of 36
            Pseudo Genes (incomplete)         :: 32 of 36
            Pseudo Genes (internal stop)      :: 2 of 36
            ##Genome-Annotation-Data-END##
FEATURES             Location/Qualifiers
     source          1..11716
                     /organism="Micropruina sp."
                     /mol_type="genomic DNA"
                     /submitter_seqid="NODE_3118_length_11716_cov_4.590172"
                     /isolate="AMR_MDS_2195"
                     /isolation_source="Activated sludge of municipal
                     wastewater treatment plants"
                     /db_xref="taxon:2737536"
                     /environmental_sample
                     /geo_loc_name="Canada"
                     /lat_lon="49.25 N 123.1 W"
                     /collection_date="2015-06-16"
                     /metagenome_source="wastewater metagenome"
                     /note="metagenomic"
     gene            194..862
                     /locus_tag="PLF56_01210"
     CDS             194..862
                     /locus_tag="PLF56_01210"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_017883916.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="thioredoxin domain-containing protein"
                     /protein_id="HOQ52212.1"
                     /translation="MKTPVKATLVTIAVVVVMVIAALIYVLVNQSQSAPPSSGGGDAS
                     PQVVRESSHVLDDGGEGAVTLVEFLDFECEACGAFFPIVEDMREQFAGEITYVVRYFP
                     LPGHFNSKNAAVAAEAAAQQDRFEDMYVRLFETQAEWGEAQESRADLFRGFAEEIGLD
                     MAAYDAAVADPATAERVELDFEEGQALGVSSTPTFFLDGELLELQEWDDLENAIQAAV
                     NGGR"
     gene            862..1881
                     /locus_tag="PLF56_01215"
     CDS             862..1881
                     /locus_tag="PLF56_01215"
                     /inference="COORDINATES: protein motif:HMM:NF019604.2"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="NAD(P)-binding domain-containing protein"
                     /protein_id="HOQ52213.1"
                     /translation="MRAGLTHRRAIIVGAGQSGLAVAAALAAEGLRPQHEFVVIDAAS
                     TGQRSWSSRWHSMELLSDARHSALSPRRLLGDQRRHPRVDEMADYLTFVEAGLGVETV
                     WGIQATGVEHRGNGSTLLLSTTAGEVQTRNVICATGAAAHPRIPEWASLLTMPGVVLH
                     SSEYLYPKQIPVGDVLIVGGGNSGVQLARELSASHTVTLSVRTRRQHRPAMSYPAAAG
                     ERVPLFSRERRPEPIFGDSYEQLRRVGVTIAAAVQDAAGAGVTFADGTQASPRSVILA
                     TGYTPGDDWLPEPARVDRPRRTLTGLPGLFVAGMPQYGCRGSDTLAGVWKDATTIAQH
                     IINRP"
     gene            1895..2611
                     /locus_tag="PLF56_01220"
     CDS             1895..2611
                     /locus_tag="PLF56_01220"
                     /inference="COORDINATES: protein motif:HMM:NF016149.2"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="copper resistance protein CopC"
                     /protein_id="HOQ52214.1"
                     /translation="MTTVTDHEHPHRSGSRRRILLLSTLTTLLLAAGLLFATAAPAAA
                     HDEIVSSSPEAGSTVSVVPEEISLTFSGEILTDFSAVIIEVVAPDGQNIASGDPVIDG
                     TTVTQAVTPGQAGVYTVRWRVVSSDGHPISNEYQYTVEAVTVPTSAPTPEATEEQTPD
                     PSPTSAANTSGEVHNGPSGGGELLPVLAVLSGVIVLGGALVVVLMVARERRRRDRAAA
                     AAAAADGGTADDQQQKENPS"
     gene            2608..2883
                     /locus_tag="PLF56_01225"
     CDS             2608..2883
                     /locus_tag="PLF56_01225"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_007078376.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="zf-TFIIB domain-containing protein"
                     /protein_id="HOQ52215.1"
                     /translation="MKCPVDGTALLITERSGVEIDYCPQCRGVWLDRGELDKIIDRAA
                     DLAGGGRQTSTAPYPREGDHRSDRDRDHHGRSGRRRKEGFLGDLFDF"
     gene            2961..3494
                     /locus_tag="PLF56_01230"
     CDS             2961..3494
                     /locus_tag="PLF56_01230"
                     /EC_number="3.4.23.36"
                     /inference="COORDINATES: protein motif:HMM:NF013421.2"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_component: GO:0016020 - membrane [Evidence IEA];
                     GO_function: GO:0004190 - aspartic-type endopeptidase
                     activity [Evidence IEA];
                     GO_process: GO:0006508 - proteolysis [Evidence IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="signal peptidase II"
                     /protein_id="HOQ52216.1"
                     /translation="MVREQTRRFAVACLAAAVVVLVDQATKAAALGGLSQEERIPLLG
                     DLLGLQLAFNPGAILSLGAGVTGLLTLLGVGAVVLLVVAAARARTGWWAMGIGLILGG
                     AIGNLIDRLFSPPGFGVGHVTDFLAYGNLFIGNLADVALGAGVIVLGLSLWTRHRRSR
                     VSADSAAPATGSVVSGS"
     gene            3491..3826
                     /locus_tag="PLF56_01235"
     CDS             3491..3826
                     /locus_tag="PLF56_01235"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_017883912.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="hypothetical protein"
                     /protein_id="HOQ52217.1"
                     /translation="MMAKQRTSSVYQRNAFAPGLLAAAVLFLAPVLMGGDWFTVVLFV
                     AAIFAVIVGWFAIQARQWWWLPVFVVIAVIWNPVFPFPFTGPVWTAAQPVAAVVFLVA
                     GALIKVKRA"
     gene            3823..4422
                     /locus_tag="PLF56_01240"
     CDS             3823..4422
                     /locus_tag="PLF56_01240"
                     /inference="COORDINATES: protein motif:HMM:NF020123.2"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_function: GO:0016491 - oxidoreductase activity
                     [Evidence IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="TlpA disulfide reductase family protein"
                     /protein_id="HOQ52218.1"
                     /translation="MIRRRVLAAMTLVAALLLAGCTSSDSLAQQYRDGSEKGYIAGDF
                     QIVEIPDADRGEPVVFEGVTETGETVSSDDYRGGVLVVNFWYAACGPCIVEAPMLEEV
                     WQEYQDQGVAFLGVNTYDQPATALSFARDNNVTYPSVIDVNDGRVKLAFAQVTPIQAT
                     PTTLVIDQEGRVAARIIGQLASASILSTLVADTLAEDSS"
     gene            4419..5186
                     /locus_tag="PLF56_01245"
     CDS             4419..5186
                     /locus_tag="PLF56_01245"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_017883918.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="cytochrome c biogenesis CcdA family protein"
                     /protein_id="HOQ52219.1"
                     /translation="MNPGAVIVDGALWVAIPVAILAGLVSFVSPCVLPLVPGYLGYLG
                     STTTTTAAPTLDGGRTVTAERARLLLGVTLFIAGFTVVFVAVTILGGTFGYLLLQYAN
                     VLTRVFGVVIIALGLVFLGFFGIAQRTLRPRAQGRTGLIGAPLLGFALGVGWTPCIGP
                     TLAAIISMSWNLGDPARAGLLGLAYSLGLGIPFLILAAGWGWASRSVTFLRRHIRALN
                     IIGGAMLIALGLLMVTGLWTALMSQLQQVVINVPLPL"
     gene            5167..6855
                     /locus_tag="PLF56_01250"
     CDS             5167..6855
                     /locus_tag="PLF56_01250"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_017883917.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_component: GO:0016020 - membrane [Evidence IEA];
                     GO_process: GO:0017004 - cytochrome complex assembly
                     [Evidence IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="cytochrome c biogenesis protein ResB"
                     /protein_id="HOQ52220.1"
                     /translation="MSPSRSDTGIDVTADPLRPGDHADSESATEITQPTLGITGWLRW
                     AWRQLTSMRTALVLLLFLAIAAVPGSLFPQRSADPNGVIQWERDNPDVFPLADAVGLF
                     DVYLSPWFSAIYLLLFTSLIGCVIPRAKHHYKALRSRPPRTPARLSRLSEYRELTLPR
                     EEGRTDPAAHAIDVAAEQLRKAGYRVERYDARGAASVSAERGYLRETGNLIFHVALVG
                     VLVSVAIGGSFAYTGQRVVVEGTTFVNALSDYSSFNPGRFVDGTGLAPYSLTLDDFQV
                     SYRLPGTPGAGQAGDFSADITIRQPGQDDRAQSVIVNYPITVDGDRIYLLGNGYAPTL
                     TIRDAAGEVVYSESQPFLPQDSNMTSLGIIKIPDGLPEQVGLVGFFYPTQGVLPSGAF
                     TSVYPDVVNPVLTLNVFSGDLGIDDGTPRSVYTLEVDGLTQHTGGDTAADSLELTPGA
                     TVDLPNGWGTITWEEVTAEEPVKRFASLQIQRDPSSGWVLAFSVLATLGLFAGLFVPR
                     RRLWVKARTTPDGVHVEYAGLARGEDPALARAVDEFATRHAHALGVDQPAKDTP"
     gene            6852..7793
                     /locus_tag="PLF56_01255"
     CDS             6852..7793
                     /locus_tag="PLF56_01255"
                     /inference="COORDINATES: protein motif:HMM:NF021214.2"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="cytochrome c oxidase assembly protein"
                     /protein_id="HOQ52221.1"
                     /translation="MTTVWIPDAPPTLGGFLTPAPLPAPVLPLLAGLLAVAYLAGAIR
                     MWVRGRGWPVWRTVSFLLGCVALAAVTGLAVENFGYALFSVFMFQQLTLIMAIPPLLV
                     LGSPGTLLLRATPHHGPGLLVLRAAHAGLRSRTARWLLSPWLAVPLYLAAFYGLYLAN
                     FADPILSTVTGHTLLEVGFLVAGMLFTIPILSSDPLPVRMSHGGRALDVFAEAALHAF
                     FGVFLMMATTTLIDGFAGPTSALGIDPIEDQRLAGGLAWSYGEAPTLLMLIYVMHRWF
                     RDDTAQAVAADRRADAHGDPELDAYNDYLTRLHQKDT"
     gene            7797..9416
                     /gene="lnt"
                     /locus_tag="PLF56_01260"
     CDS             7797..9416
                     /gene="lnt"
                     /locus_tag="PLF56_01260"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_017883914.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_component: GO:0016020 - membrane [Evidence IEA];
                     GO_function: GO:0016410 - N-acyltransferase activity
                     [Evidence IEA];
                     GO_process: GO:0006497 - protein lipidation [Evidence
                     IEA];
                     GO_process: GO:0042158 - lipoprotein biosynthetic process
                     [Evidence IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="apolipoprotein N-acyltransferase"
                     /protein_id="HOQ52222.1"
                     /translation="MRTPLPTPAPAAAPEQETPRRTVAAWSLPVWAAVLASAAAGLLL
                     DLASAPVGWWPLTFVSVTVALVALIGRSIGGALLVGTVFGAVFYTTHLVWVGEFLGPV
                     PWLALAGLEAVLFGAGAVPIALAYRWTARYPARGLVQLLAVPPLIGVLWTAREVVMGA
                     WPYSGFPWARLGMTQVGGPLAEAVSWTSVTGLSLLIVILCASVVQWIRAGGIRFMLGL
                     HPAVSVAALLVIAPQFPTAPAGEFRVGWVQGNGPTGYFDDKVPGDVLAAQTAATVPLY
                     GQPMDLLAWPEGGVDADPLSDPAAAEALDRVVRSAGAPLLMNAATTRGDDVFNTSLLW
                     TADPTGRQWHDKVNPVPFGEYVPDRWFYELIVPDLVGLIQREYTPGSNPPLVQVGDVG
                     VGLAICFDVIYDAVIWDGARAGAEVFVFQTNNADFRGTDENLQQLAFARMRAIETGRA
                     VVNVSTVGTSQVIAPDGTTVDSIGVDTVDASITTVPLRTGLTPAVILGPWLTALIVLA
                     AAGALTAAGFSHRARTRAAAADRSTDPGRHP"
     gene            9569..10819
                     /locus_tag="PLF56_01265"
     CDS             9569..10819
                     /locus_tag="PLF56_01265"
                     /EC_number="3.4.-.-"
                     /inference="COORDINATES: protein motif:HMM:NF013700.2"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_function: GO:0046872 - metal ion binding [Evidence
                     IEA];
                     GO_process: GO:0006508 - proteolysis [Evidence IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="M23 family metallopeptidase"
                     /protein_id="HOQ52223.1"
                     /translation="MSRRTLLTAGTLSAAAVLVAGPLLPAAFAATYPSWEDVQAAKAN
                     EATKAAEVQRIQGLIQSLTGEVARTRTAAEHAAGAFYTAQQEYFDASIRADTLQFQAD
                     LKAQNATDAANKAGRIAAQLYRDGGDTTSLELFLSGSAATADDLLSRLGVMDKLLERN
                     QAVYAAALTARDAAQSLTDQAVVARDERDRLQQVAEQKMLESQQAADAAQTALDAQTL
                     HLGELQAQLAALEDTTAKTIADYQAGVEAARIAEEQRRAAERAEAERLAASGGGGGGV
                     VSSGWARPTSGHRTSGFGPRSSQCGNGYCSTSYHYGVDLSGGCGAGIYAAAAGTVVYA
                     GYNGGYGNYIKIDHGGGIGTGYAHIRPGGFYVGYDQRVNAGDLIGSEGNTGNAFGCNL
                     HFEAYVNGSPVNPITFLADRGVST"
     gene            10841..>11716
                     /locus_tag="PLF56_01270"
     CDS             10841..>11716
                     /locus_tag="PLF56_01270"
                     /EC_number="3.4.-.-"
                     /inference="COORDINATES: protein motif:HMM:NF013700.2"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_function: GO:0046872 - metal ion binding [Evidence
                     IEA];
                     GO_process: GO:0006508 - proteolysis [Evidence IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="M23 family metallopeptidase"
                     /protein_id="HOQ52224.1"
                     /translation="MTDQTTPPGGGDQPQTPPTRRSLRRAATPPATSITTRPPRRPPA
                     ETSARGRVIALRPVRSLAVLTVVTGLIATFALPAYAAWQPADLEIPTLQQVASEDAQS
                     LIVTSDATGTQLTRESYSATTQAEIDQKKAAEAAAERARASAQVASVPFDYSIVPQGS
                     GAVRWPVGGPFTVTDRFGARGGAHMGTDMVAAGGTPVYASVDGVVRISQDSYGGYGVT
                     VVVESVLNGQQVSTVYPHMQTGSRQVAAGQTVTAGQLVGLVGSTGRSTANHLHFEVYL
                     DGTAVDSLAWLEANAG"
BASE COUNT         1644 a         4197 c         4005 g         1870 t
ORIGIN      
        1 ttctcggtgc agcgccacgg atgtcttcgc tgctccctgc ttcggcagcg cgcgtgcatc
       61 cgatcgtgct ctcgttgacg caactgggtc tttcccgaac ctgatatctg gcacgccacc
      121 gcgttgggtg gcgcttcgtc ccgggctgtg tgcccgggtg tgtcctgtgc cgtgtcttgg
      181 tttggagtac ccgatgaaaa cccctgtgaa ggcgacgctc gtcaccattg ccgttgtggt
      241 ggtgatggtg atcgccgcgt tgatctatgt ccttgtcaat cagagccagt ccgctccacc
      301 ttcatctggg ggcggtgacg catccccgca ggtggtgcgg gagagctcgc atgtcctcga
      361 cgacggcggg gagggcgctg tcaccctggt ggagttcctc gacttcgagt gcgaggcgtg
      421 tggcgcgttc ttcccgatcg tggaggatat gcgggagcag ttcgctgggg agatcacgta
      481 tgtggtccgc tacttcccgc tgcccggtca cttcaattcg aagaacgcgg cggtcgcggc
      541 ggaagcggcc gcgcagcagg accggttcga ggacatgtat gtccggttgt tcgagacgca
      601 ggcggagtgg ggtgaggcgc aggagtctcg cgccgacctg ttccgtggct tcgcggagga
      661 gatcggtctg gacatggccg cctatgacgc cgccgtggcg gacccggcta cggcggagcg
      721 ggtggagctg gatttcgagg agggccaggc gctcggggtg agcagcacgc cgacgttctt
      781 cctcgacggt gagctgctcg agctgcagga gtgggacgac ctcgagaacg ccatccaggc
      841 tgcggtgaac ggtggccggt gatgcgggcg ggtttgacgc atcggcgggc gatcatcgtc
      901 ggcgccgggc agtctgggct cgcggttgcc gcggctctgg ccgcggaagg gctgcggccg
      961 cagcacgagt ttgtggtgat cgacgccgcc agcacggggc agcgttcctg gtcctcacgg
     1021 tggcattcga tggagcttct cagcgacgcc cggcatagcg cgctgtctcc gcggcggctg
     1081 ctgggtgacc agcgccggca tccgcgagtg gacgagatgg cggactacct caccttcgtg
     1141 gaagccgggc tgggcgtgga gacggtctgg ggcatccagg cgaccggggt ggagcatcgc
     1201 gggaacggct cgactctgct gctgtcgacg acggcagggg aggtgcagac ccgcaacgtg
     1261 atctgcgcga cgggcgcggc cgcgcacccg cggattccgg agtgggcgtc tctgctgacg
     1321 atgcccgggg tggtgctgca cagcagcgag tacctgtacc cgaagcagat ccccgtcggc
     1381 gacgtgctca tcgtgggcgg cgggaacagc ggtgtgcagc tggcccgcga actgtccgcg
     1441 tcgcacaccg tgacgctgtc cgtgcgaacc cggcggcagc atcgccccgc gatgagctat
     1501 cccgccgcgg cgggggagag ggtgccgctg ttctcgcggg agcgtcgccc cgagccgatc
     1561 ttcggcgaca gctatgagca gctgcgccgc gtcggggtca cgatcgcagc tgcggtgcag
     1621 gacgcggccg gcgccggggt gaccttcgcc gatggcacgc aggcatcccc ccgctcggtg
     1681 atcctcgcca ccggctacac ccctggcgac gactggctcc cggaaccggc ccgcgtcgac
     1741 cggccgcggc gcaccctgac cggcctgccc gggctgttcg tggcggggat gccgcagtac
     1801 ggctgccgcg gctccgacac cctcgccggg gtctggaaag acgcgacgac gatcgcccag
     1861 cacatcatca accggccctg aaaggaccac cgccttgacc accgtcaccg accacgaaca
     1921 cccgcaccga tccggctcac ggagacgcat cctcctcctg tccactctga cgacgctgct
     1981 gctggccgcc gggctcctgt tcgccaccgc ggcgcccgca gccgcgcacg atgagatcgt
     2041 ctcctcctcc ccggaagccg gatccacggt cagcgtggtc ccggaggaga tttcgctgac
     2101 gttcagcggc gagatcctca ccgatttcag cgccgtgatc attgaggtgg tcgccccgga
     2161 cgggcagaac atcgcatcgg gggatccggt catcgacggg accacggtca cccaggcggt
     2221 gacgcctggt caggcgggcg tgtacacggt gcggtggcgg gtcgtgtcca gcgacgggca
     2281 cccgatcagc aacgagtacc agtacaccgt cgaagcggtc accgtcccca ccagcgcccc
     2341 tacccccgaa gcgaccgagg agcagacacc cgacccgtct cccacatctg ccgccaatac
     2401 gtccggagag gttcacaacg ggccatccgg aggtggggaa ctcctccccg tccttgcggt
     2461 cctgagtggc gtaatcgtgc tgggcggcgc gctggtggtc gtgctcatgg tggccagaga
     2521 gcgtcgtcgc cgcgaccgag cagccgcggc cgcggccgcc gcggatggtg gaaccgccga
     2581 cgaccagcaa cagaaggaga acccctcatg aaatgtccgg tcgacgggac cgccctgctg
     2641 atcacggaac gctccggggt cgagatcgac tactgcccgc agtgccgggg cgtctggctg
     2701 gaccgtggcg agctcgacaa gatcatcgac cgcgccgccg accttgccgg cggcggccgg
     2761 caaaccagca ccgcccccta tccgcgcgag ggcgaccacc gttctgaccg cgacagggat
     2821 catcacggtc gctccgggcg tcggcggaag gaagggttcc tcggtgacct cttcgacttc
     2881 tgacaccaaa aacctcttcc tgatagccgg tctgatgacc gggttatcag cggccctcgc
     2941 gaacgcagga ggagccgctg atggttcgtg agcagacccg gcggttcgcg gtcgcgtgcc
     3001 tggccgccgc tgtggtggtc ctggtcgacc aggcgacgaa ggcggccgca ctgggagggt
     3061 tgagtcagga ggagcggatc ccgctgctgg gggatctgct tgggctgcag ctcgcgttca
     3121 accccggcgc gatcctctcc ctcggagccg gggtcaccgg gctgctcact ctcctcgggg
     3181 tcggtgccgt ggtgctgctg gtcgtcgccg ccgcgcgggc gcggaccgga tggtgggcga
     3241 tggggatcgg cctgatcctc gggggcgcga tcgggaacct gatcgaccgg ctgttctccc
     3301 cccctgggtt cggggtcggg cacgtcaccg acttcctcgc ctacgggaac ctgttcatcg
     3361 gcaacctcgc cgacgtcgcc ctcggtgccg gcgtcatcgt cctcggcctg agcctgtgga
     3421 cccgacaccg ccgctcccgc gtcagcgcgg acagcgcggc gcctgcgacg ggctccgtgg
     3481 tgagcgggtc atgatggcga agcagcggac ctcgtccgta taccagcgca acgcgttcgc
     3541 gccggggctg ctcgcggcgg cggtgttgtt cctcgcgccg gtactgatgg gcggggactg
     3601 gttcaccgtt gtgctgttcg tcgccgcgat cttcgcggtc atcgtggggt ggttcgcgat
     3661 ccaggcgcgg cagtggtggt ggttgcctgt gttcgtcgtg atcgccgtga tttggaaccc
     3721 ggtgttcccg ttcccgttca ccggcccggt gtggactgcg gcgcagccgg tcgccgcggt
     3781 cgtgttcctg gtcgccggcg ccctgatcaa ggtcaagcgc gcatgatccg ccgccgcgtc
     3841 cttgccgcca tgacgctggt cgcggcgctg ctgctggccg ggtgcacgtc cagcgactcg
     3901 ctcgcgcagc agtaccggga cggaagcgag aagggctaca tcgccggtga cttccagatc
     3961 gtcgagatcc cggatgccga ccgcggggag ccggtggtgt tcgagggcgt caccgagacc
     4021 ggggagacgg tgtccagtga cgactaccgc ggcggggtgc tggtggtgaa cttctggtac
     4081 gccgcgtgcg ggccgtgcat cgtcgaagcc ccgatgctcg aggaggtctg gcaggagtat
     4141 caggaccagg gcgtcgcgtt cctcggggtg aacacctacg accagccagc caccgcactg
     4201 tcgttcgccc gtgacaacaa cgtcacctac ccgagcgtga tcgacgtgaa cgacggacgg
     4261 gtcaagctcg cgttcgcgca ggtcactccg atccaggcca cccccaccac cttggtcatc
     4321 gaccaggagg ggcgggtcgc ggcgcggatc atcgggcagt tggccagcgc gtcgatcctg
     4381 tccaccctgg tcgccgacac cctcgccgag gacagctcat gaatccggga gcggtgatcg
     4441 tcgacggcgc cctgtgggtc gcgatcccgg tcgcgatcct cgccgggctg gtgtcgttcg
     4501 tgtccccgtg cgtgctgccg ctggtgcctg gctacctcgg ctacctcggc agcaccacca
     4561 cgacgaccgc ggcccccacg ctggacgggg ggcgcacggt gacggcggag cgcgcgcggc
     4621 tgctgctggg cgtgacattg ttcatcgccg ggttcaccgt ggtgttcgtc gccgtcacga
     4681 tcctcggcgg caccttcggg tacctgctgc tgcaatacgc caatgtgctc actcgcgtct
     4741 tcggggtcgt catcatcgcc ctcggcctgg tgttcctcgg cttcttcggc attgcccagc
     4801 gcaccctccg ccctcgcgcc cagggtagga ccgggctgat cggggcgccg cttctcgggt
     4861 tcgcgctcgg cgtcggctgg accccctgta tcggcccgac gctcgcagcg atcatctcta
     4921 tgtcgtggaa cctcggtgac cccgcccgcg cgggcctgct cgggctcgcg tactcgctgg
     4981 gcctgggcat cccgttcctg atcctcgcgg ccggatgggg gtgggcgtcc cgatcggtga
     5041 cgttcctgcg ccgccacatc cgcgccctga acatcatcgg cggggccatg ctcatcgccc
     5101 tcggcctgct gatggtgacc gggctgtgga cggcactgat gtcgcagctg caacaggtgg
     5161 tgatcaatgt ccccctcccg ctctgacacc ggcatcgacg tcaccgccga cccgctacgc
     5221 cccggcgacc acgccgacag cgaatccgcg acggagatca cccagccgac gctgggcatc
     5281 accgggtggt tgcggtgggc atggcggcag ctgaccagca tgcgcaccgc attggtcctg
     5341 ctgctgttcc tcgcgatcgc cgccgtcccc gggtccctgt tcccgcagcg cagcgccgac
     5401 cccaacggtg tcatccagtg ggagcgggac aaccctgacg tgttccccct cgccgatgcg
     5461 gtcggcctgt tcgacgtgta cctgtcgccg tggttctccg cgatctatct gctgctgttc
     5521 acctccctga tcggctgcgt gatcccgcgc gccaagcacc actacaaggc gctccgctcc
     5581 cgcccccctc gcaccccggc ccggctgtcg cggctctcgg agtaccggga actgaccttg
     5641 ccgagagagg aagggaggac cgacccggcc gcgcacgcga tcgacgtcgc ggcagagcag
     5701 ctgcgcaagg ccggataccg ggtggagcga tacgacgccc gcggagccgc gtcggtgtcg
     5761 gccgagcgcg gctacctgcg cgagaccggc aacctgatct tccacgtcgc cctcgtcggc
     5821 gtgctggtct cggtcgcgat cggcgggtcg ttcgcgtata ccgggcagcg ggtggtggtg
     5881 gagggcacca cgttcgtgaa cgcgctcagc gactactcct cgttcaaccc cggccgtttc
     5941 gtcgacggca cgggccttgc cccctactcg ctcaccctcg acgacttcca ggtctcctac
     6001 cggctgcccg gcacccccgg cgcaggccag gccggagact tctccgccga catcaccatc
     6061 cgccaacccg ggcaggacga tcgggcgcag agcgtgatcg tgaactaccc gatcaccgtc
     6121 gacggcgatc gcatctacct gctcggcaac ggctacgccc ccaccctcac gatccgcgac
     6181 gccgccggcg aggtggtgta cagcgagtcg cagccgttcc tgccgcagga ctccaacatg
     6241 acctcgctgg gcatcatcaa gatcccggac gggctgcccg aacaggtcgg gctggtcggg
     6301 ttcttctacc ccacccaggg ggtgttgccc tccggcgcgt tcacctccgt ctacccggac
     6361 gtggtcaatc cggtgctgac tctcaacgtg ttcagcgggg atctcggcat cgacgacggc
     6421 actccgaggt cggtgtacac gctcgaggtc gacgggctca cccagcacac cggcggcgac
     6481 accgccgccg actccctcga gctcaccccc ggcgccaccg tcgacctgcc gaacgggtgg
     6541 ggcacgatca cctgggagga ggtcacggcg gaggagccgg tgaagcggtt cgcgtcgctg
     6601 cagatccaac gcgaccccag cagcggctgg gtgctcgcgt tctccgtgct cgccaccctc
     6661 ggcctgttcg ccggcctgtt cgtgccccgc cgccggctct gggtgaaagc ccgcaccacc
     6721 ccggatggtg tgcacgtcga gtacgcggga ctggcccgcg gcgaagaccc cgctttggcg
     6781 cgcgccgtcg acgagttcgc gacccgccac gcgcacgccc tcggcgtaga ccagcctgcg
     6841 aaggacacac cgtgacgacc gtgtggatcc cggacgcccc gccgaccctc ggcgggttcc
     6901 tcaccccggc cccgcttccg gctccggtgc tcccgctcct cgcgggactc ctcgcggtcg
     6961 cctacctggc cggtgcgatc cggatgtggg tccgcggccg cggctggccg gtgtggcgga
     7021 cggtcagctt cctgctcggc tgcgtcgccc tcgccgcggt caccggcctg gcggtggaga
     7081 acttcgggta cgccctgttc tcggtgttca tgttccagca gctcaccctg ataatggcga
     7141 tcccgccgct gctggtcctc ggctctcccg gcaccctgct gctgcgcgcc accccgcacc
     7201 acggcccagg tctcctggtg ctccgcgccg cgcacgccgg gctacgcagt cgcaccgcac
     7261 ggtggctgct cagcccgtgg ctggcggtgc cgctatacct ggccgcgttc tacggtctgt
     7321 acctggcgaa cttcgccgat ccgatcctgt ccaccgtcac cggccatacc ctcctcgagg
     7381 tcgggttcct ggtcgccggg atgctgttca ccatcccgat cctgtcgtcg gacccgctgc
     7441 cggtgcggat gagccacggc ggccgcgccc tggacgtgtt cgccgaggcc gccctgcacg
     7501 ccttcttcgg cgtcttcctg atgatggcca ccactaccct catcgacggg ttcgccggcc
     7561 cgacgagcgc gctgggcatc gacccgatcg aagaccagcg cctcgccggc gggctggcct
     7621 ggtcctacgg cgaggccccc accttgctga tgctcatcta cgtcatgcac cgctggttcc
     7681 gcgacgacac cgcccaagcg gtcgccgccg accgtcgcgc cgacgcacac ggcgaccctg
     7741 agctcgacgc gtacaacgac tacctcaccc gactccacca gaaagacacc tgaccgatgc
     7801 gcacgccttt gccgacaccc gccccggccg ccgcgccgga gcaggagacg ccgcgccgca
     7861 ccgtggcggc atggtcgctg ccggtgtggg cggcggtgct ggcatccgcg gccgccggcc
     7921 tgctgctgga tctcgcctcc gcaccggtgg ggtggtggcc gttgacgttc gtgagtgtca
     7981 cggtcgccct ggtggccctg atcggccgca gcatcggcgg ggcgctgctg gtcggcactg
     8041 tgttcggcgc cgtgttctac acgacccatc tggtgtgggt gggggagttc ctcggcccgg
     8101 tgccgtggct tgccctcgcc gggctggaag cggtcctgtt cggcgcgggc gcggtgccga
     8161 tcgcgctcgc ctaccggtgg accgcccgct atccggcccg cggcctcgtg cagctgctcg
     8221 cggtaccgcc gctgatcggg gttctgtgga cggcccgtga ggtggtgatg ggtgcgtggc
     8281 cgtactccgg gttcccgtgg gcgcgcctcg ggatgaccca ggtgggcggc ccgctcgcgg
     8341 aggccgtgtc gtggacgagc gtgaccggcc tgtccctgct gatcgtcatt ctgtgcgcct
     8401 ctgtggtgca gtggatccgc gccggcggca tccgcttcat gctcgggttg caccctgctg
     8461 tgagcgtcgc cgctctcctg gtgatagcgc cgcagttccc caccgcgccg gccggggagt
     8521 tccgcgtggg atgggtgcag ggcaacggcc ccaccgggta cttcgacgac aaggtgcccg
     8581 gcgacgtcct ggccgcgcag accgccgcca cggtgccgct gtacgggcag ccgatggatc
     8641 tgctggcctg gccggagggc ggcgtcgacg ccgacccgct cagtgatccc gccgccgccg
     8701 aggcgttgga ccgggtcgtg cgctcggccg gggcgccgtt gctgatgaac gccgccacca
     8761 cccgcggcga cgatgtcttc aacacgtccc tgctgtggac cgcggatccc acgggccggc
     8821 agtggcacga caaggtcaac ccggtcccgt tcggcgagta cgtgccggac cggtggttct
     8881 acgagctgat cgtccccgac ctggtgggct tgatccaacg cgaatacacc cccggcagca
     8941 acccgccgct cgtgcaggtc ggcgacgtcg gggtggggtt ggcgatctgc ttcgacgtga
     9001 tctacgacgc cgtgatctgg gacggcgccc gcgccggcgc agaggtgttc gtgttccaga
     9061 ccaacaacgc cgacttccgc ggcaccgacg agaacctgca gcagctcgcg ttcgcccgga
     9121 tgcgcgctat cgaaaccggc cgcgcggtgg tcaacgtctc cacggtgggc accagccagg
     9181 tcatcgcccc ggacggcacc accgtcgaca gcatcggcgt cgacaccgtt gacgcgtcga
     9241 tcaccaccgt cccgctgcgc accggactca ccccggcggt gatcctcggt ccctggctca
     9301 ccgctctcat cgtcctcgct gccgccggcg ctctcaccgc ggcggggttc tcccaccgtg
     9361 cccgcacgcg tgcggccgcc gcagatcgtt cgactgatcc ggggaggcac ccatgaacac
     9421 gccacggatg aggcctgccg gaggtcgact ggcctggtcg ctgcgaacgc tggcgacggg
     9481 cagcgatctc acccttcctg gtgacctgac ggcggaggag tgtgggtgcg cgcccacgcc
     9541 cgcggagagc cgcgcattcc gggccggggt gagccggcgc actctgctga cggcgggaac
     9601 attgagcgcg gccgccgtcc tggttgccgg cccgctgctg ccggcagcgt tcgcggcgac
     9661 ctatccgtcc tgggaggacg tgcaggccgc gaaagcgaac gaggccacca aagctgccga
     9721 ggtgcagcgg atccagggcc tcatccaaag tcttaccggc gaggtggcgc gcacccgcac
     9781 cgccgccgag catgccgccg gcgcgttcta caccgcccag caggagtact tcgacgcatc
     9841 catccgcgcc gacacgttgc agttccaggc cgacctgaaa gcgcagaacg cgaccgacgc
     9901 ggcgaacaag gccggccgca tcgccgcgca gctgtaccgc gacgggggcg acaccacctc
     9961 cctcgagctg ttcctctccg ggtcggccgc gaccgcggac gatctcctgt cccggctggg
    10021 agtgatggac aagctgctgg aacgcaacca ggccgtctac gctgcggcgc tcaccgcccg
    10081 cgatgccgcc cagagcctga ccgatcaggc ggtcgtggcc cgggatgagc gcgaccggct
    10141 gcagcaggtc gccgagcaga agatgctcga gtcgcagcag gccgccgatg ctgcgcagac
    10201 cgcgctggac gcgcagaccc tccacctcgg cgagctgcag gcccagctcg ccgccctcga
    10261 ggacaccacc gcgaagacca tcgccgacta tcaggccggg gtggaggctg cccgcatcgc
    10321 cgaggagcag cggcgagcgg cggagcgcgc cgaagcggaa cggctcgccg cctcgggagg
    10381 tggtggtggg ggtgtggtca gctccgggtg ggcacggccg acctccgggc accgcacctc
    10441 ggggttcggg ccgcgcagct cccagtgtgg caacgggtac tgctcgacca gctaccacta
    10501 cggcgtcgac ctcagcggcg gatgcggcgc ggggatctac gccgccgcgg ccggcaccgt
    10561 cgtctacgcc gggtacaacg gcggctacgg caactacatc aagatcgacc acggcggcgg
    10621 gatcggcacc ggatacgccc acatccgccc cggcggcttc tacgtcggat acgaccaacg
    10681 ggtgaacgcc ggcgacctga tcggcagcga aggcaacacc ggcaacgcgt tcggctgcaa
    10741 cctgcacttc gaggcctacg tgaacggctc cccggtcaac cccatcacct tcctcgccga
    10801 ccgcggcgtg tccacctgac ccgaaacgaa ccaggacatc atgactgacc agaccacccc
    10861 acccggcggc ggcgaccagc cccagacgcc ccccacgcgc cgcagcctcc gccgcgccgc
    10921 aaccccgcca gccaccagca tcaccacccg ccccccacgg cgcccgcccg cagaaacgtc
    10981 cgcccgtggt cgggtaatcg cgctgcgccc agtgcgctcc ctggcggtcc tgacggtggt
    11041 caccggcctg atcgccacct tcgccctccc cgcgtatgcc gcctggcagc cggcggacct
    11101 cgagatcccg acactgcagc aagtcgcgtc cgaggacgcg caatccttga tcgtcacctc
    11161 cgacgcgact gggacgcagc tcacacggga gagctactcg gccaccaccc aggcggagat
    11221 cgaccagaag aaggcggcag aagcagccgc agaacgtgcc cgcgcctccg cacaagtcgc
    11281 ctcggtgccc ttcgactaca gcatcgtgcc ccaaggcagt ggagccgtgc ggtggccggt
    11341 gggcggcccg ttcaccgtga ccgacaggtt cggcgctcga ggcggcgcgc atatgggcac
    11401 cgacatggtc gctgccggcg gaacccccgt ctacgcgtcc gtcgacggcg tcgtgcggat
    11461 ctcgcaggac agctacggcg gctacggggt gaccgtcgtg gtggagtccg tgctgaacgg
    11521 gcaacaggtg agcacggtgt acccgcacat gcagaccgga agccggcagg tcgccgccgg
    11581 gcagaccgtc acggccgggc agctggtcgg actcgtcggc agcacgggac gctccaccgc
    11641 gaaccatctg cacttcgagg tctacctcga cggaaccgcc gtggactcgc tggcctggct
    11701 ggaagcgaac gcgggc
//
LOCUS       DAOSUG010000019        11650 bp    DNA     linear   ENV 10-JUN-2024
DEFINITION  MAG TPA_asm: Micropruina sp. isolate AMR_MDS_2195
            NODE_3142_length_11650_cov_4.013195, whole genome shotgun sequence.
ACCESSION   DAOSUG010000019 DAOSUG010000000
VERSION     DAOSUG010000019.1
DBLINK      BioProject: PRJNA925477
            BioSample: SAMN32864262
            Sequence Read Archive: SRR4236673
KEYWORDS    WGS; ENV; Metagenome Assembled Genome; MAG; Third Party Data; TPA;
            TPA:assembly.
SOURCE      Micropruina sp. (wastewater metagenome)
  ORGANISM  Micropruina sp.
            Bacteria; Bacillati; Actinomycetota; Actinomycetes;
            Propionibacteriales; Nocardioidaceae; Micropruina.
REFERENCE   1  (bases 1 to 11650)
  AUTHORS   Abdulkadir,N., Saraiva,J.P., Zhang,J., Stolte,S., Gillor,O.,
            Harms,H. and Rocha,U.
  TITLE     Genome-centric analyses of 165 metagenomes show that mobile genetic
            elements are crucial for the transmission of antimicrobial
            resistance genes to pathogens in activated sludge and wastewater
  JOURNAL   Microbiol Spectr 12 (3), e0291823 (2024)
   PUBMED   38289113
REFERENCE   2  (bases 1 to 11650)
  AUTHORS   Abdulkadir,N.
  TITLE     Direct Submission
  JOURNAL   Submitted (24-MAR-2023) Environmental Microbiology, Helmholtz
            Center for Environmental Research -UFZ, Permoserstr 15, Leipzig
            04318, Germany
COMMENT     The annotation was added by the NCBI Prokaryotic Genome Annotation
            Pipeline (PGAP). Information about PGAP can be found here:
            https://www.ncbi.nlm.nih.gov/genome/annotation_prok/
            
            ##Genome-Assembly-Data-START##
            Assembly Method        :: SPAdes v. 3.11.1
            Genome Representation  :: Full
            Expected Final Version :: Yes
            Genome Coverage        :: Not Applicable
            Sequencing Technology  :: Illumina MiSeq
            ##Genome-Assembly-Data-END##
            
            ##Genome-Annotation-Data-START##
            Annotation Provider               :: NCBI
            Annotation Date                   :: 05/04/2023 04:57:10
            Annotation Pipeline               :: NCBI Prokaryotic Genome
                                                 Annotation Pipeline (PGAP)
            Annotation Method                 :: Best-placed reference protein
                                                 set; GeneMarkS-2+
            Annotation Software revision      :: 6.5
            Features Annotated                :: Gene; CDS; rRNA; tRNA; ncRNA
            Genes (total)                     :: 2,776
            CDSs (total)                      :: 2,750
            Genes (coding)                    :: 2,714
            CDSs (with protein)               :: 2,714
            Genes (RNA)                       :: 26
            tRNAs                             :: 24
            ncRNAs                            :: 2
            Pseudo Genes (total)              :: 36
            CDSs (without protein)            :: 36
            Pseudo Genes (ambiguous residues) :: 0 of 36
            Pseudo Genes (frameshifted)       :: 2 of 36
            Pseudo Genes (incomplete)         :: 32 of 36
            Pseudo Genes (internal stop)      :: 2 of 36
            ##Genome-Annotation-Data-END##
FEATURES             Location/Qualifiers
     source          1..11650
                     /organism="Micropruina sp."
                     /mol_type="genomic DNA"
                     /submitter_seqid="NODE_3142_length_11650_cov_4.013195"
                     /isolate="AMR_MDS_2195"
                     /isolation_source="Activated sludge of municipal
                     wastewater treatment plants"
                     /db_xref="taxon:2737536"
                     /environmental_sample
                     /geo_loc_name="Canada"
                     /lat_lon="49.25 N 123.1 W"
                     /collection_date="2015-06-16"
                     /metagenome_source="wastewater metagenome"
                     /note="metagenomic"
     gene            complement(<1..974)
                     /locus_tag="PLF56_01275"
     CDS             complement(<1..974)
                     /locus_tag="PLF56_01275"
                     /inference="COORDINATES: protein motif:HMM:NF013741.2"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="CNNM domain-containing protein"
                     /protein_id="HOQ52225.1"
                     /translation="MTDIEVFELAVAAACVLVAALLVTAEAALQSFSKSRAQRLLDEG
                     RGGAQALVNITQDPAPTLNTLLFVRILAEIVAIVLVTLFVFGRFEATWERVLYSVGIM
                     VLVSFILLGVAPRTLGRQNADSVALGAARPLGMLTTVLGPIPQVMILIGNALTPGKGF
                     ADGPFSSEAELRELVDYAEASDVIEAGERKMIHSVFDLGDTIVKEVMVPRTQMVYIER
                     TKTVRQAVSLALRSGFSRIPVIGDDLDDICGVLYLKDAIRRMYDNPRAQATETVESLM
                     RKPTFCPDSKPVDELLREMQLTRSHVVIVVDEFGGTSWSYSSTISPTST"
     gene            1127..1888
                     /locus_tag="PLF56_01280"
     CDS             1127..1888
                     /locus_tag="PLF56_01280"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_008362298.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_process: GO:0006810 - transport [Evidence IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="sulfite exporter TauE/SafE family protein"
                     /protein_id="HOQ52226.1"
                     /translation="MPVPDLTTLLWLVAAGVLTGIVGYGAGLASLVSFPALLAVGLPP
                     LTANLTNTVALVGTTIGGVSAARTELVAVRRHLLPYGLIALTGGAIGAGLLLAAPAEV
                     FEKVVPWLVLLGSVVLVLSPWLRRLQAGRISHRHPGVGAAIGLVAIYCGYFGAGAGVL
                     VLAVLSAVLEHTLAELTALRSVLLGCANLVAAGIFMATGGVAWAQALALGVGAVAGAA
                     LGPWIMRRVPETPARVVVALAGVALAAKLFLDYYR"
     gene            1969..2574
                     /locus_tag="PLF56_01285"
     CDS             1969..2574
                     /locus_tag="PLF56_01285"
                     /inference="COORDINATES: protein motif:HMM:NF012691.2"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="hypothetical protein"
                     /protein_id="HOQ52227.1"
                     /translation="MADVVNLVILGASGDLTHRLLLPGLGTLLKAEPKRAVHLVGAAA
                     DDLTQEQWQQRVRDSIQSSGCDSTVIDKLAAGTTYARVDATSADDLWALLKGLSGQVV
                     LYFALPPAISMKACEALSTLTLPEQLFLAIEKPFGTDPSSARQFNELLARFVPEDRVF
                     RVDHFLGKSTILNLLGLRFTNRFFEPVWNRDNVERIEIVVD"
     gene            2584..3330
                     /locus_tag="PLF56_01290"
     CDS             2584..3330
                     /locus_tag="PLF56_01290"
                     /inference="COORDINATES: protein motif:HMM:NF014800.2"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="hypothetical protein"
                     /protein_id="HOQ52228.1"
                     /translation="MEGRAGYYDHSGALKDMIQSHLLLVMALLCMEPPNRIAAEDLRS
                     LEADVLRATRLWQDDPVKASRRARYTAGAIDAHDVPNYVDERGVDPARATETLAEVTV
                     EIHSARWHGVPIRMRSGKALGDGVRSITVVFKDAFQPEGFANQALPNVVVIGMADESV
                     QVRLNTNGADDKFTLEDTVLSADLGESPIRPYGEILAHIMDGNPALSVRGDVAEDCWR
                     ILGPVLDAWGDGRVPMDEYRAGSSGPTNWN"
     gene            complement(3398..3853)
                     /gene="ybeY"
                     /locus_tag="PLF56_01295"
     CDS             complement(3398..3853)
                     /gene="ybeY"
                     /locus_tag="PLF56_01295"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_013864282.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_function: GO:0004540 - ribonuclease activity [Evidence
                     IEA];
                     GO_function: GO:0046872 - metal ion binding [Evidence
                     IEA];
                     GO_process: GO:0042254 - ribosome biogenesis [Evidence
                     IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="rRNA maturation RNase YbeY"
                     /protein_id="HOQ52229.1"
                     /translation="MSVEVNNESGVPVDEQRLVALALFALDALRIHPQAELSLLLVDE
                     PTMAAYHEKYMDEPGPTDVLSFPMDELRPPKDGEEPPLGMLGDIVLCPTVTSAQAAAS
                     NRTPDEEADYLLIHGLLHLLGYDHAEPDEKKVMFDLNDRVIAAWNAQRG"
     gene            complement(3855..4868)
                     /locus_tag="PLF56_01300"
     CDS             complement(3855..4868)
                     /locus_tag="PLF56_01300"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_016456306.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_function: GO:0005524 - ATP binding [Evidence IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="PhoH family protein"
                     /protein_id="HOQ52230.1"
                     /translation="MPTADSSVTKPTGVRRVTVPASVDMVNVLGPRDEFLRILERELS
                     ADVHVRGNEITFTGDPADVTVAAEVLTELVTIVRTGQGLTADAVERVVGMTAQQAETL
                     HAAEVLTQNILSTRGRTIRPKTLNQKRYVDAIDAHTVVFGIGPAGTGKTYLAMAKAVQ
                     ALQAKEVNRIILTRPAIEAGERLGFLPGTLTDKIDPYLRPLYDALHDMVDPDSVPRLL
                     TAGTIEVAPLAYMRGRSLNDAFIILDEAQNTSMEQMKMFLTRLGFGSKIVVTGDVTQV
                     DLPTGTRSGLRMVQGILDGVDDIAFCRLTNHDVVRHKLVGRIVAAYDRFESTQSTPSK
                     QER"
     gene            4938..5558
                     /locus_tag="PLF56_01305"
     CDS             4938..5558
                     /locus_tag="PLF56_01305"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_012922857.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_function: GO:0005524 - ATP binding [Evidence IEA];
                     GO_function: GO:0016301 - kinase activity [Evidence IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="nucleoside/nucleotide kinase family protein"
                     /protein_id="HOQ52231.1"
                     /translation="MLGGMHVDEACERAAALASGGRRLLGLVGPPGAGKSTLSAALQR
                     HLGEAMVVVGMDGFHLANAELIRLGRHERKGAPDTFDVDGYVALLARLRSQSGPLYAP
                     RFDRELEESIGSAVRVEPWTPLVVTEGNYLLYDGPGWDAVRPLLDEVWYLELDDVERR
                     RRLVQRRLGHGDALEHARFWVETVDEPNAALVAGTRQRADSVVFVD"
     gene            complement(5525..6751)
                     /locus_tag="PLF56_01310"
     CDS             complement(5525..6751)
                     /locus_tag="PLF56_01310"
                     /inference="COORDINATES: protein motif:HMM:NF019310.2"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_function: GO:0022857 - transmembrane transporter
                     activity [Evidence IEA];
                     GO_process: GO:0055085 - transmembrane transport [Evidence
                     IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="MFS transporter"
                     /protein_id="HOQ52232.1"
                     /translation="MTQAVVARRVRRQRVAVSVLFVLAGAVMGGWAGRIPSIRGQLGV
                     SDGEWGLIVLGMPLGVLGSLLVLQRVITRTGARRPAVIGAIGMLLITPAAAASANIVV
                     MVGFLLALGVAQGLLFGPMNALAVEVERDYGRTIISSFHAWYSAGQFVGGLLGVLAGL
                     LVLSPTIQLGASSLLLGALFAATLRFLPDTHKAADDPSTLDEPVTAVPGEAPRRSWTP
                     QLVLLAAIGFLTSINEGGAVQWSAQYTVTLGGSIALGSLTLVCYSLSIAGVRLIGDRI
                     VERIGRRLFLQLSAGFAALGLGLALLVGTVPAALIGFACVGIGSGCIVPTVMTLAGNQ
                     PGVPSAKAVALISLGEWPAFLLGPPVIGGLAELLGLRAGLGVIVLGAAAIVLLATFVR
                     EPAPVAQSTNTTESAR"
     gene            complement(6837..7037)
                     /locus_tag="PLF56_01315"
     CDS             complement(6837..7037)
                     /locus_tag="PLF56_01315"
                     /inference="COORDINATES: ab initio
                     prediction:GeneMarkS-2+"
                     /note="Derived by automated computational analysis using
                     gene prediction method: GeneMarkS-2+."
                     /codon_start=1
                     /transl_table=11
                     /product="hypothetical protein"
                     /protein_id="HOQ52233.1"
                     /translation="MSNDPNAGGDISSGFGESEVKGPVSDEKAEQMLHGGSPSDDDSQ
                     APGDDYTETDRATNEQDLSQDQ"
     gene            complement(7109..8092)
                     /gene="pip"
                     /locus_tag="PLF56_01320"
     CDS             complement(7109..8092)
                     /gene="pip"
                     /locus_tag="PLF56_01320"
                     /EC_number="3.4.11.5"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_004811668.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_function: GO:0004177 - aminopeptidase activity
                     [Evidence IEA];
                     GO_process: GO:0030163 - protein catabolic process
                     [Evidence IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="prolyl aminopeptidase"
                     /protein_id="HOQ52234.1"
                     /translation="MTTTTELYPPLEPYASGRLEVGDSQNLYWEECGNPEGKPVVFVH
                     GGPGGGCSPLHRRYFDPAAYRIVLVDQRGCGRSTPHASEPDADLSSNTTWHLVADLER
                     LRQHRGIERWQVFGGSWGSSLALAYAETHPEVVTELVLRGIFTLRRSELDWYYNGGAA
                     NLAPAWWQRFEAPLRARFGELPAGFDRIAGYHELLFDPDPAVHGPAGVAWTTWEAATS
                     TLEFDQTMVDDFSDPAFALAFARIENHYFVHGGWFREGQLIAEVDRIRHIPAVIVQGA
                     YDLPCPAITAWQLHQAWPEADFRLVLAGHAVTEPAIAPELLAATDGFARRG"
     gene            complement(8076..8516)
                     /locus_tag="PLF56_01325"
     CDS             complement(8076..8516)
                     /locus_tag="PLF56_01325"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_014846743.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="DUF5709 domain-containing protein"
                     /protein_id="HOQ52235.1"
                     /translation="MTDFDPSLPDESEQLDQLQPEETLIDRGVDDVLDEGYVAPEKWS
                     PAQSFGNTAAEMRHGETIEQRLAQEEPDVAPATGPWKDDGDDNRQVGKRRAGRLVDAD
                     GGYGEADVESESIGYEVGISGGAASAEEAAMHVIDDYEDDDDDD"
     gene            8596..9327
                     /locus_tag="PLF56_01330"
     CDS             8596..9327
                     /locus_tag="PLF56_01330"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_006680956.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_process: GO:1990397 - queuosine salvage [Evidence IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="queuosine precursor transporter"
                     /protein_id="HOQ52236.1"
                     /translation="MSSQPTAPRPVYDLVLALFCGLLLISNVGATKLIQFGPSWTVGG
                     FPVLPIITDGGAFLFPLTYILGDVLAEVYGLKKAQRAIWTGFGLAFLMSLTFAVVAAA
                     PPAEGWPNNDAWNAVLGFVPRIVIASLAGYLAGQSLNALVLVTIKQRFGPERLWVRLV
                     GSTVVGEFADTLVFCLIAFGPLGAFLGGGSIDLPTLVNYTLVGFVYKVTVEVVFLPVT
                     YRVIPWVRRREGLADPVAPAVSALR"
     gene            complement(9706..10485)
                     /locus_tag="PLF56_01335"
     CDS             complement(9706..10485)
                     /locus_tag="PLF56_01335"
                     /inference="COORDINATES: protein motif:HMM:NF015669.2"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="META domain-containing protein"
                     /protein_id="HOQ52237.1"
                     /translation="MAASRLLIITLALLLTACAGTATPGGSPSASAPTLEGTSWVVTQ
                     IKGAATLAGHQPTIAFSGGRASGKASCNSYSGDFTQSGGTLTLGPVAMTAMACADEAV
                     NTQEHAFTGALGEVTQVRGTADGAELLNAGGAAVLTLATAPKAGPKPLIGTPWTLTGI
                     VANQGVSSVVSGTTVTMTLTQDALSGKACNTFRGPITVKDGALTVGPLATTKMACPTE
                     QEGKQEATVLATLADVTGYTIDGDTLTLSTPTSTGLVFRAS"
     gene            complement(10666..>11650)
                     /locus_tag="PLF56_01340"
     CDS             complement(10666..>11650)
                     /locus_tag="PLF56_01340"
                     /inference="COORDINATES: protein motif:HMM:NF013961.2"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=2
                     /transl_table=11
                     /product="transglutaminase-like domain-containing protein"
                     /protein_id="HOQ52238.1"
                     /translation="DAASVFLAPTLQRLGAGDAEQWRQLVQVAQSLKQGAWSDGTRAG
                     EEQYLPGHGQYRLNQFVTGQQFVGSDEQYAATFALMASRLGYPARVVFGAEIPAGGEV
                     RGKNVQAWVELSVQGHGWVSVPVSVFAPDRSKKPDPNQRQNNEQESIQNVPPPNPVPP
                     PGTYEEMTDTDPASARAELPIADQFWRTFWSVARWVGPPLGVLALIVGTILGLKALRG
                     RRRRSRGAPSTRIAGGWAEVLDAARDVGRPVPGAATRLEQSALLGRADVHDLAVRADG
                     AVFRGGEIDPQQITDYWTAVRAARTALVRDLPWWRRPLARLNLASLVSRRA"
BASE COUNT         1708 a         3981 c         4112 g         1849 t
ORIGIN      
        1 gtactcgtcg gcgagatcgt cgacgagtac gaccaggagg tgccgccgaa ctcgtcgacc
       61 acgatcacca cgtgcgagcg ggtgagctgc atctcgcgca gcagctcgtc caccggtttg
      121 ctgtcggggc agaaggtcgg cttgcgcatc agcgactcga cggtttcggt ggcctgcgcc
      181 cgcgggttgt cgtacatgcg ccgaatcgcg tctttgaggt agagcacgcc gcagatgtcg
      241 tccagatcgt caccgatcac cggaatgcgg ctgaaacccg accgcagcgc caacgaaacc
      301 gcttgtcgca cggttttggt gcgctcgatg tagaccatct gggtgcgcgg caccatgacc
      361 tctttgacga tcgtgtcgcc caggtcgaac accgagtgga tcatcttgcg ttcgccggcc
      421 tcgatcacat cggacgcctc ggcgtagtcg accagttcgc gcagttcggc ctcggaactg
      481 aacgggccgt cggcgaagcc cttgccgggg gtgagcgcgt tgccgatcag gatcatcacc
      541 tgcgggatcg ggccgagcac ggtggtgagc atgccgagcg gacgggcggc tcccagcgcc
      601 acggagtcgg cgttctgccg gcccagggtg cggggtgcca cgccgagcag tatgaacgag
      661 accagcacca tgatgccgac cgagtagagc acccgctccc aggtcgcctc gaagcgtccg
      721 aacacgaaca gggtgaccag cacgatggcc acgatctcgg cgaggatgcg tacgaacagc
      781 agggtgttga gcgtgggtgc cggatcctgg gtgatgttca ccagcgcctg ggcgccgccg
      841 cggccctcgt cgagcaggcg ctgggcgcgg ctcttcgaga acgattgcag cgccgcctct
      901 gcagtgacca gcagcgcggc caccagcacg caggcagctg caacggccaa ctcgaacact
      961 tcgatgtcgg tcaacagggt gtcattcctc gattcgcgcc cggaaagtcg aaaccgcagt
     1021 ctacgacagc atcacccggc caggcctcgg ccgcactgcc cggaccgtcg ggtcgcagtc
     1081 tgggacgcgt ccagccgggt tggtcgcgcg gaccaggatg gcggatgtgc cagtgcccga
     1141 cctcaccacc ctgctgtggc tggtcgcggc cggcgtcctc accggcatcg tcggttacgg
     1201 ggccggcctg gcctcgctgg tgtcgtttcc cgccctgctg gccgtggggc tgcccccgct
     1261 gaccgcgaac ctgacgaaca ccgttgcgct cgtcggcacc acgatcggcg gcgtatcggc
     1321 cgcgcgcaca gagttggtgg ccgtgcgccg gcacctgctg ccctacggcc tgatcgccct
     1381 caccggcggc gcgatcggtg cgggcctgct gctggcagca ccggccgagg tgttcgagaa
     1441 ggtggtgccg tggctggtgc tgctgggttc ggtggtgctg gtgctgtcgc cgtggctgcg
     1501 ccgcctgcag gccggacgga tctcgcaccg ccacccgggc gtcggcgcgg cgatcgggct
     1561 ggtggcgatc tactgcggat acttcggtgc gggtgccggc gtgctggtgc tggccgttct
     1621 cagtgccgtg ctcgagcaca ccctggccga actgaccgcg ttgcgcagcg ttctgctcgg
     1681 ttgcgccaac ctggtggcgg cgggcatctt catggccacc ggcggcgtgg cctgggcgca
     1741 ggcactggcc ctgggcgtgg gcgcggtggc cggcgcggca ctcggtccct ggatcatgcg
     1801 ccgagtgccc gagaccccgg cccgggtcgt ggtggcgttg gccggcgtcg ccctggccgc
     1861 gaagctcttt ctcgactact accgctgatt cgggcgccgt aagctctgcg accccagctg
     1921 tggggatcca tgggtagcct ggccgagttt ccagacaagg agtgcggcgt ggctgatgtg
     1981 gtgaacctgg tgattctggg ggcgtccggt gacctgaccc atcggctgct gctgccgggg
     2041 ctcggcaccc tgctgaaggc cgagcccaag cgggccgtgc atctggtggg tgcggccgcc
     2101 gacgatctga cccaggaaca gtggcaacag cgggtcaggg actcgattca atccagcggc
     2161 tgcgactcca cggtgatcga caagctggcc gcgggcacca cctatgcgcg cgtcgatgcc
     2221 accagcgccg acgacctgtg ggcgctgctg aagggcctga gcggccaggt ggtgctctat
     2281 ttcgccttgc cgccggccat cagcatgaag gcctgcgagg cgctgagcac gctgaccctg
     2341 cccgagcaac tgttcctggc gatcgagaag ccgttcggca ccgacccgtc cagcgctcgg
     2401 cagttcaacg agttgcttgc tcgattcgtg cccgaggacc gggtgttccg ggtcgatcac
     2461 tttctgggca agtccaccat tctcaacctg ctcgggctgc gattcaccaa ccggttcttc
     2521 gagccggtgt ggaatcgtga caatgtcgaa cgcatcgaga tcgtggtcga ctagaccctg
     2581 gcactggagg gccgggccgg ttactacgac cactcgggtg cgctcaagga catgatccag
     2641 agtcacctgc tgctggtgat ggcgctgctg tgcatggagc cgccgaaccg tatcgccgcc
     2701 gaagacctgc gcagcctcga agccgatgtg ctgcgcgcca cccggttgtg gcaggacgac
     2761 ccggtgaagg cctcccgacg agcccgctac accgccggcg ccatcgacgc ccacgacgtg
     2821 cccaactatg tggacgagcg cggtgtcgat cccgcgcggg ccaccgagac cctggccgag
     2881 gtgaccgtcg agatccacag tgcgcgctgg cacggggtgc cgatccggat gcgcagcggc
     2941 aaggcgctcg gcgatggggt gcgatccatc accgtggtat tcaaggacgc ctttcagccg
     3001 gagggcttcg ccaaccaggc actgccgaac gtcgtggtga tcggcatggc cgacgaatcg
     3061 gtgcaggtgc gcctcaacac caacggcgcc gacgacaagt tcaccctcga agacacggtg
     3121 ctgagcgccg atctgggcga gagccccatc cgtccttacg gtgagattct ggcgcacatc
     3181 atggacggca atccggcgtt gtcggtgcgc ggtgacgtcg ccgaggactg ctggcgcatc
     3241 ctgggtccag tactggacgc gtggggtgac gggcgcgtgc cgatggacga gtaccgcgcc
     3301 gggtcgtccg gccccacgaa ctggaactga ccccgagggg ccacgcacgc gcgagcgcgc
     3361 aggtacggcc ccactgaccc atcgctggac gagttggtca gccccgctgc gcattccacg
     3421 ccgcgatgac gcggtcgttc aggtcgaaca tgaccttctt ctcatcgggc tcggcatggt
     3481 cgtacccgag caggtgcagc aggccgtgga tcagcaggta gtcggcctct tcgtcggggg
     3541 tgcggttcga ggcggccgcc tgcgccgacg tgacggtggg gcacaggacg atgtcgccga
     3601 gcatgcccag cgggggttcc tcgccgtctt tgggggggcg cagctcgtcc atcggaaagc
     3661 tgagcacgtc ggtggggccg ggctcgtcca tgtacttctc gtgataggcg gccatggtcg
     3721 gctcgtcgac cagcagcagc gaaagctcgg cctgaggatg gatgcgcagg gcgtccaggg
     3781 cgaacagggc gagagcgacc agccgctgtt cgtcgacggg cacgcccgac tcgttgttga
     3841 cctcgacgct cacctcagcg ctcctgcttg ctcggcgtgg actgggtgga ttcgaaccgg
     3901 tcgtaggcgg cgacgatgcg tccgaccagc ttgtgccgca ccacgtcatg gttggtcagc
     3961 cggcagaacg cgatgtcgtc gacaccgtcc agaatgccct gcaccatgcg caggcccgag
     4021 cgggtgccgg tgggcaggtc gacctgggtg acgtcgccgg tgaccacgat cttggagccg
     4081 aaccccagcc gggtcaggaa catcttcatc tgctccatcg acgtgttctg cgcctcgtcc
     4141 agaatgatga acgcgtcgtt caacgagcga ccgcgcatgt aggccagcgg cgccacctcg
     4201 atggtgccgg cggtcagcag ccgcggcacc gaatcggggt cgaccatgtc gtgcagggcg
     4261 tcgtagagcg gccgcaggta ggggtcgatc ttgtcggtga gggtgccggg caagaagccg
     4321 agccgctcgc cggcctcgat cgcgggccgg gtcaggatga tgcggttgac ctctttggct
     4381 tgcagtgcct ggacggcctt cgccatggcc aggtaggtct tgccggtgcc ggccggaccg
     4441 atgccgaaca ccacggtgtg ggcgtcgatc gcatccacgt agcgcttctg gttgagcgtt
     4501 ttgggacgga tcgtgcgacc gcgggtgctc agaatgttct gggtgagcac ctcggcggca
     4561 tgcagggttt cggcctgttg cgcggtcatg ccgaccaccc gctcgacggc gtcggcggtg
     4621 aggccctgcc cggtgcgcac gatggtgacc agctcggtca gcacctcggc ggcgaccgtg
     4681 acgtcggcgg gatcgccggt gaacgtgatc tcgttgccgc gcacatgcac gtcggcgctc
     4741 agttcgcgct cgagaatgcg aaggaactcg tcgcggggcc ccagcacatt gaccatgtcg
     4801 accgacgcgg gaaccgtgac ccgacgcact ccggtgggtt tggtcacgct gctgtcggct
     4861 gtaggcatgt tcctccttga aactgtgcgc ctgggacgaa gagcatccta accctcccgt
     4921 ccgacaacag atccggcatg ctgggcggca tgcatgtgga cgaggcctgc gaacgggccg
     4981 cggcgctggc ctcgggcgga cgtcgcctgc tcgggttggt gggcccgccg ggagccggca
     5041 agtctacgct gtcggcggcg ttgcagcggc acctgggcga ggcgatggtc gtggtcggca
     5101 tggacgggtt tcacctggcc aacgccgaac tgatccggct cggacggcac gagcgcaagg
     5161 gagcgcccga caccttcgac gtggacggct acgtggcgct gctggcccgg ctgcgcagcc
     5221 agagcggtcc gctgtacgcg ccgcggttcg atcgcgagct ggaggagtcg atcggcagcg
     5281 ccgtccgggt cgagccgtgg acgccgctgg tggtcaccga gggcaactac ctgctctacg
     5341 acgggcccgg ctgggacgcg gtgcgtccgc tgttggacga ggtgtggtac ctcgagctgg
     5401 acgatgtcga gcgccggcgg cggttggtgc agcgccggct cgggcacggt gacgccctcg
     5461 agcacgcccg gttctgggtc gagaccgtgg acgaacccaa cgccgccctg gtcgccggca
     5521 cccgtcagcg cgcggattcg gtggtgttcg tcgactgagc caccggcgcc ggttcgcgca
     5581 cgaaggtggc cagcagcacg atggccgcgg cgccgagcac gatcacgccg agccccgcgc
     5641 gcaggcccag cagttcggcc aacccgccga tcacgggggg cccgagcaga aaagcgggcc
     5701 actcccccag gctgatcagc gccacggcct tcgcgctggg cacgccgggc tggttgccgg
     5761 ccagggtcat cacggtgggc acgatgcagc cgctgccgat gcccacgcag gcgaagccga
     5821 tcagcgcggc cggcacggtg ccgaccaaca gcgccaggcc cagcccgagc gcggcgaaac
     5881 cggccgagag ctgcaggaac agccgccgtc cgatgcgttc gacgatgcgg tcgccgatca
     5941 gtcgtacacc ggcgatcgag agcgagtagc agaccagggt gagcgagccg agggcgatgg
     6001 agccgcccag ggtgaccgtg tactgggcgc tccactgcac cgcgccgccc tcgttgatcg
     6061 aggtgagaaa gccgatcgcg gccaacagca ccaactgggg cgtccatgac cggcggggtg
     6121 cctcgccggg cacggcggtg acgggttcgt cgagggtcga cggatcgtcc gcggccttgt
     6181 gggtgtcggg cagaaaccgc agggtggcgg cgaaaagcgc acccagcaac aggctggagg
     6241 caccgagttg gatcgtgggt gacagcacca gcaggccggc gagcaccccg agcagcccgc
     6301 cgacgaactg tccggcgctg taccaggcgt ggaaggagct gatgatggtg cggccgtagt
     6361 cgcgttcgac ctcgacggcc agcgcgttca tcggcccgaa cagcaggccc tgcgccacgc
     6421 cgagggccag caaaaagccg accatcacga cgatgttggc cgatgctgcc gccgcgggcg
     6481 tgatcagcag catgccgatg gcgccgatca cggccgggcg ccgcgcgccg gtacgggtga
     6541 tcacccgctg cagcaccagc agcgagccca gcacgcccag cggcatgccg agcacgatca
     6601 gcccccactc accatcgctc acgccgagct gcccgcgaat ggacgggatg cgcccggccc
     6661 agccgcccat cacggctccg gcgagcacga acagcaccga gaccgcgacc cgctgccggc
     6721 gcacccggcg cgcaacgaca gcctgcgtca cgttctgcac ctcgctgcat cgaaggggga
     6781 tgggcccgat cgagggccgt cggccagcct atcggtgaca gcgggggcga tcgcccctac
     6841 tggtcctggc tcaggtcctg ttcgttggtc gcccggtcgg tctcggtgta gtcgtcgccg
     6901 ggggcctgcg agtcgtcatc ggagggcgac ccgccgtgca gcatctgctc ggccttctcg
     6961 tccgagaccg gacccttgac ctcggattcg ccgaagccgg acgagatgtc gccgccggcg
     7021 ttggggtcgt tgctcatcgg aactccttcg atcgattgcc tgcacgttac cggcacccgg
     7081 cgccccgatg acagggtcgc cgcccgaatc aaccccttcg ggcgaagccg tcggtcgccg
     7141 ccagcaactc gggcgcgatg gccggttcgg tcaccgcgtg gcccgccaac accagccgga
     7201 aatccgcctc cggccaggcc tgatgcagct gccatgcggt gatcgccggg cagggcaggt
     7261 cgtaggcgcc ctgcacgatc accgcgggga tgtgccggat gcggtccacc tcggcgatca
     7321 gctgaccctc gcgaaaccag ccgccgtgca cgaaatagtg attctcgatc cgcgcgaagg
     7381 ccagcgcgaa agcggggtcg ctgaagtcgt ccaccatggt ctggtcgaac tccagcgtgg
     7441 acgtcgcggc ctcccacgtc gtccaggcga ccccggccgg gccgtgtacc gctgggtcgg
     7501 gatcgaacag cagttcgtgg tagccggcga tgcggtcgaa gccggccggc agctcaccga
     7561 accgagcccg caggggtgcc tcgaagcgtt gccaccaggc cggcgccagg ttggccgcgc
     7621 cgccgttgta gtaccagtcg agttcactgc gccgcagcgt gaagatgccg cgcagcacca
     7681 actcggtgac cacctcggga tgggtttcgg cgtaggccag ggccagcgag ctgccccacg
     7741 aaccgccgaa cacctgccag cgctcgatgc cgcgatgctg ccgcagccgc tccaggtcgg
     7801 cgaccaggtg ccaggtggtg ttgctcgaca ggtcggcgtc gggctcgctg gcgtgcgggg
     7861 tgcttcgtcc gcaaccgcgc tgatcgacca gcacgatgcg atacgccgcc ggatcgaagt
     7921 agcggcggtg caacggtgag cacccgcccc cggggccgcc gtgcacgaac accaccggct
     7981 tgccctcagg gttgccgcac tcctcccagt agaggttttg tgagtcgccg acctcgagcc
     8041 gtccggacgc gtagggttcg agcggcggat acagctcagt cgtcgtcgtc atcgtcctcg
     8101 tagtcgtcga tcacatgcat ggcggcctct tccgcactcg cagcgccgcc cgagatgccg
     8161 acctcgtagc cgatgctctc ggactcgacg tccgcctcgc cgtagccgcc gtccgcgtcg
     8221 accagccggc cggcccgccg cttgccgacc tgccggttgt cgtcgccgtc gtccttccag
     8281 ggcccggtgg ccggcgccac gtccggctct tcctgggcca gtcgctgctc gatggtttcg
     8341 ccatggcgca tctctgcggc ggtgttgccg aaactctgcg ccggtgacca cttctcgggc
     8401 gctacatagc cctcgtcgag cacgtcgtcg accccgcggt cgatcagcgt ctcttcgggc
     8461 tgcaactggt ccagttgctc ggactcatcg ggcaaactcg ggtcgaagtc ggtcatgtgc
     8521 ctagcctgcc atctcgcctc ccgcagggcg aggacgcacg cgaacaggta cggatttcgc
     8581 ctaggctcgc ctgccatgag ctcgcaaccg acggccccgc gccccgtcta cgatctggtg
     8641 ctcgcgctgt tctgcggcct gctgctgatc tcgaacgtgg gcgcgaccaa gctgatccag
     8701 ttcggcccga gctggacggt gggtggcttt ccggtgctgc cgatcatcac cgacggcggc
     8761 gcgttcctgt ttccgctcac ctacattctg ggcgacgtgc tggccgaggt gtacgggctg
     8821 aagaaggcac agcgggccat ctggacgggg ttcgggctcg ccttcctgat gtcgctgacc
     8881 ttcgccgtgg tggcggccgc accgcccgcg gaggggtggc ccaacaatga cgcgtggaac
     8941 gcggtgctcg gcttcgttcc gcggatcgtg atcgccagcc tggccggcta cctggccggg
     9001 cagagcctca acgccctggt gctggtgacg atcaagcagc gcttcggccc cgaaaggttg
     9061 tgggtacggc tggtcggctc gacggtggtc ggtgagttcg ccgacaccct ggtgttctgc
     9121 ctgatcgcat tcgggccgct cggcgcgttt ctgggcggcg gcagcatcga cctgccgacc
     9181 ctggtcaact acacgctggt cggtttcgtc tacaaggtca ccgtcgaggt ggtgttcctg
     9241 ccggtcacct accgggtcat accgtgggtt cgtcgtcggg agggcctggc ggacccggtt
     9301 gcgccggccg tttccgcgct ccggtgatcc gttgccacag ccggcgccag acgccgggtc
     9361 cgtcccttcg caccggcagc gggccggggg tcgcccaccc cggcaacgcc ggatcggcgg
     9421 gcggaacctg cagtgcgtcg cggagcacgg tgcggtcgtc cggaacagcc tgaccggtat
     9481 cggcctgacc gggttcggac gactgcgcgg tggccgcgag tggctgtggc accggcggcg
     9541 acccatcgag agcggcgccg caacgggcgc aacgcggggc cgccaacgtc tgctgcaggc
     9601 cgcaggcagg gcaggacgga gcggtcatga acctagtgtc gcccgctcac cgcacgaagt
     9661 cacgggcagc gctccccgtt gcggtccccg tccggtggcg tcgattcagg aggcccggaa
     9721 caccagcccg gtggacgtgg gcgtcgacag ggtgagcgtg tcgccgtcga tggtgtagcc
     9781 ggtcacgtcc gccagggtgg cgagcacggt cgcctcctgc ttgccctcct gctcggtcgg
     9841 gcaggccatt ttggtcgtgg ccaacgggcc gacggtgagc gcgccgtcct tcaccgtgat
     9901 cggcccacgg aacgtgttgc aggccttgcc gctgagggcg tcctgcgtga gcgtcatggt
     9961 caccgtggtg ccgctcacca cggacgacac gccctgattg gcgacgatgc cggtgagcgt
    10021 ccagggcgtg ccgatcagtg gcttggggcc ggccttcggg gccgtggcca gggtcaacac
    10081 ggcggcgccg ccggcgttca gcagttcggc accgtcggcc gtgccgcgca cctgggtgac
    10141 ctcgcccagt gcgccggtga aggcgtgctc ctgggtgttc accgcctcat ccgcgcacgc
    10201 catggcggtc atcgcgaccg ggccgagggt gagggtgccg ccgctctggg tgaaatcgcc
    10261 gctgtaggag ttgcaactcg ccttgccgct ggcacgtccg ccgctgaagg cgatggtcgg
    10321 ttggtggccg gccagggtcg ccgcaccctt gatctgggtg acgacccacg acgtgccctc
    10381 cagggtgggc gcggaggcgg acggcgaccc accgggcgtc gcggtgccgg cgcaggcggt
    10441 gagcagcaga gccagggtga tgatcagcag ccgtgatgcg gccatcgggc gtcctctccg
    10501 acgcggtgcg ccgcgcgtgt cgcggcccac ccatggtagg gccgccgccg accgacgggc
    10561 gtgagcgcaa cgaggttgtt cggggccgga acgcagaccg gtggtgtgtc ccagtccgag
    10621 tcacctggcg ggagtcggga accgccccaa acccccacgt cctgctcagg cccgacgcga
    10681 taccagggag gccaggttga gccgggccag cggacggcgc caccagggca ggtcgcgcac
    10741 cagggccgtg cgggccgcac gcaccgccgt ccagtagtcc gtgatctgct gcgggtcgat
    10801 ctcgccgccc cggaacaccg cgccgtcggc ccgtaccgcc aggtcgtgga cgtccgcgcg
    10861 gcccagcagc gcgctctgct ccagccgggt cgccgccccc ggcaccggac gtcccacgtc
    10921 gcgcgcggcg tccagcacct cggcccagcc gccggcgatc cgcgtcgacg gtgccccgcg
    10981 gctgcgccgc cggcggcccc gcagcgcctt caaccccagg atcgtgccga cgatcagcgc
    11041 cagcacgccc agcggaggac ccacccagcg cgccacgctc cagaacgttc gccagaactg
    11101 gtcggcgatc ggcaactcgg cccgggccga ggccgggtcg gtgtccgtca tctcttcgta
    11161 ggtgccgggc ggcggcaccg ggttgggcgg cggcacgttc tggatcgatt cctgctcgtt
    11221 gttctgccgc tggttcggat cgggcttctt gctgcgatcg ggagcgaaca ccgacaccgg
    11281 cacgctcacc cagccgtggc cctgcaccga caactccacc caggcctgga cgttcttgcc
    11341 ccgcacttcg ccgccggccg ggatctcggc cccgaacacc acccgcgccg ggtaccccaa
    11401 ccggctcgcc atcagggcga acgtggccgc gtactgctcg tccgagccga cgaactgctg
    11461 cccggtgacg aactggttga gccggtactg gccgtgaccg ggcaggtact gctcctcgcc
    11521 ggcccgggtg ccgtcgctcc acgcgccctg cttgagcgac tgcgccacct ggaccagttg
    11581 tcgccactgc tcggcatcgc ccgcgcccag ccgctgcagg gtgggcgcca ggaacaccga
    11641 cgcggcatcg
//
LOCUS       DAOSUG010000020        11582 bp    DNA     linear   ENV 10-JUN-2024
DEFINITION  MAG TPA_asm: Micropruina sp. isolate AMR_MDS_2195
            NODE_3160_length_11582_cov_4.816952, whole genome shotgun sequence.
ACCESSION   DAOSUG010000020 DAOSUG010000000
VERSION     DAOSUG010000020.1
DBLINK      BioProject: PRJNA925477
            BioSample: SAMN32864262
            Sequence Read Archive: SRR4236673
KEYWORDS    WGS; ENV; Metagenome Assembled Genome; MAG; Third Party Data; TPA;
            TPA:assembly.
SOURCE      Micropruina sp. (wastewater metagenome)
  ORGANISM  Micropruina sp.
            Bacteria; Bacillati; Actinomycetota; Actinomycetes;
            Propionibacteriales; Nocardioidaceae; Micropruina.
REFERENCE   1  (bases 1 to 11582)
  AUTHORS   Abdulkadir,N., Saraiva,J.P., Zhang,J., Stolte,S., Gillor,O.,
            Harms,H. and Rocha,U.
  TITLE     Genome-centric analyses of 165 metagenomes show that mobile genetic
            elements are crucial for the transmission of antimicrobial
            resistance genes to pathogens in activated sludge and wastewater
  JOURNAL   Microbiol Spectr 12 (3), e0291823 (2024)
   PUBMED   38289113
REFERENCE   2  (bases 1 to 11582)
  AUTHORS   Abdulkadir,N.
  TITLE     Direct Submission
  JOURNAL   Submitted (24-MAR-2023) Environmental Microbiology, Helmholtz
            Center for Environmental Research -UFZ, Permoserstr 15, Leipzig
            04318, Germany
COMMENT     The annotation was added by the NCBI Prokaryotic Genome Annotation
            Pipeline (PGAP). Information about PGAP can be found here:
            https://www.ncbi.nlm.nih.gov/genome/annotation_prok/
            
            ##Genome-Assembly-Data-START##
            Assembly Method        :: SPAdes v. 3.11.1
            Genome Representation  :: Full
            Expected Final Version :: Yes
            Genome Coverage        :: Not Applicable
            Sequencing Technology  :: Illumina MiSeq
            ##Genome-Assembly-Data-END##
            
            ##Genome-Annotation-Data-START##
            Annotation Provider               :: NCBI
            Annotation Date                   :: 05/04/2023 04:57:10
            Annotation Pipeline               :: NCBI Prokaryotic Genome
                                                 Annotation Pipeline (PGAP)
            Annotation Method                 :: Best-placed reference protein
                                                 set; GeneMarkS-2+
            Annotation Software revision      :: 6.5
            Features Annotated                :: Gene; CDS; rRNA; tRNA; ncRNA
            Genes (total)                     :: 2,776
            CDSs (total)                      :: 2,750
            Genes (coding)                    :: 2,714
            CDSs (with protein)               :: 2,714
            Genes (RNA)                       :: 26
            tRNAs                             :: 24
            ncRNAs                            :: 2
            Pseudo Genes (total)              :: 36
            CDSs (without protein)            :: 36
            Pseudo Genes (ambiguous residues) :: 0 of 36
            Pseudo Genes (frameshifted)       :: 2 of 36
            Pseudo Genes (incomplete)         :: 32 of 36
            Pseudo Genes (internal stop)      :: 2 of 36
            ##Genome-Annotation-Data-END##
FEATURES             Location/Qualifiers
     source          1..11582
                     /organism="Micropruina sp."
                     /mol_type="genomic DNA"
                     /submitter_seqid="NODE_3160_length_11582_cov_4.816952"
                     /isolate="AMR_MDS_2195"
                     /isolation_source="Activated sludge of municipal
                     wastewater treatment plants"
                     /db_xref="taxon:2737536"
                     /environmental_sample
                     /geo_loc_name="Canada"
                     /lat_lon="49.25 N 123.1 W"
                     /collection_date="2015-06-16"
                     /metagenome_source="wastewater metagenome"
                     /note="metagenomic"
     gene            <1..309
                     /locus_tag="PLF56_01345"
     CDS             <1..309
                     /locus_tag="PLF56_01345"
                     /inference="COORDINATES: ab initio
                     prediction:GeneMarkS-2+"
                     /note="Derived by automated computational analysis using
                     gene prediction method: GeneMarkS-2+."
                     /codon_start=1
                     /transl_table=11
                     /product="hypothetical protein"
                     /protein_id="HOQ52239.1"
                     /translation="PESRAYDRTLVLLYIGTLAVNSLLLYVMSVVVRRTPATWRPDDP
                     PTRRMGEAVNLSMTILFSLAAVLALLFPAINMATMLLLFFTGPLANLLLRRSTPPSAP
                     "
     gene            430..1671
                     /locus_tag="PLF56_01350"
     CDS             430..1671
                     /locus_tag="PLF56_01350"
                     /EC_number="2.1.-.-"
                     /inference="COORDINATES: protein motif:HMM:NF024880.2"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_function: GO:0008168 - methyltransferase activity
                     [Evidence IEA];
                     GO_function: GO:1904047 - S-adenosyl-L-methionine binding
                     [Evidence IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="class I SAM-dependent methyltransferase"
                     /protein_id="HOQ52240.1"
                     /translation="MLTALHALIHSDDPTATRAFLRDVLRWPFVSEGAEGDTGVGGAG
                     TGGTDAAEWLILRSGPSEVAVHPTTWQAGDEPRSVPRQHTVALICDDLATTMDELRGR
                     GAEFSTAPRDAGYGVAVMLKVPGADDLLLYQAAHAVAYSLPDDAGHQQRSAGPGSGGH
                     EHGDQQSGEQGQGHQSGEQGHRHGEQGHRHREHEHEHEHEHGEHQHGGRRMPVTDDEW
                     HQHYAPEAIWSGNPNGALVAELAGLTPGRAVDVGCGEGADAVWLAGQGWQVTAFDVAE
                     SALERGRAAAESAGVEVEWVRAGLLSSELQAGAFDLVSACYPALQRTPERLAEQALLR
                     LVAPGGRLLMLAHADVDRTTALEHGFDPDDYLDPGDLLTAASDGWSVERHERRPRRID
                     GGAGAGHHTDIVLVLRREQSA"
     gene            1668..2489
                     /locus_tag="PLF56_01355"
     CDS             1668..2489
                     /locus_tag="PLF56_01355"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_017935214.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_function: GO:0016491 - oxidoreductase activity
                     [Evidence IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="oxidoreductase"
                     /protein_id="HOQ52241.1"
                     /translation="MSGQGVALVTGASSGIGAESAKHLAAAGFLVYAAARRTDRLGEL
                     ASGSIRPLTMDVTDDDSVTAGVQQVLAEAGRIDALVNNAGYGSYGAVEDVPLDEARRQ
                     FEVNLFGLARLTQLVIPGLRQQGSGRIVNISSMGGRFAMPFGAWYHATKFAVEGFSDA
                     LRQELAPFGIQVVVIEPGGIKTEWGGISAASALERSGEGPYAARVQRIARALAGSDRR
                     FGSEPGVVAKAITTAVTARRPRTRYAIGAGAKPAIYARRFLPDRVMDALIGRVLG"
     gene            2572..3774
                     /locus_tag="PLF56_01360"
     CDS             2572..3774
                     /locus_tag="PLF56_01360"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_013862681.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_function: GO:0004497 - monooxygenase activity [Evidence
                     IEA];
                     GO_function: GO:0005506 - iron ion binding [Evidence IEA];
                     GO_function: GO:0016705 - oxidoreductase activity, acting
                     on paired donors, with incorporation or reduction of
                     molecular oxygen [Evidence IEA];
                     GO_function: GO:0020037 - heme binding [Evidence IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="cytochrome P450"
                     /protein_id="HOQ52242.1"
                     /translation="MSSSAESRRAVRPDEPDEPRIEHVGHVWRVRSAAAARQVLRERR
                     TTTQAGFTAEAIPKGYLKHHPILMSDGPLHDEQRTKVGRFFAPKVVEERYLDLMESCA
                     DRLLGDAAASGMVRLDDLALLYTVEVTAQAVGLTESSVPAMAKRLVTFFNQPPFDITR
                     PDLGRTPRQWAQAALNGLLPIGRFWWSDVRPAIRVRRRKPREDVISHLIAEGYTNADI
                     LVECVTYGTAGMVTTREFIAMAAWHLLGNEPLRARYLVAGQAERLAILNEIIRLEPVV
                     GHLYRRVQQPVTFTDQGHEHTAQPGDLIDLCIRPANADPETVGERPLQLCPGRPLPKG
                     VNEAVLSFGDGAHKCPGQPLAMLESDALLRRLLAREVSIVTEPSIEWDDLIAGYALRG
                     FDMQIGDG"
     gene            4052..4675
                     /locus_tag="PLF56_01365"
     CDS             4052..4675
                     /locus_tag="PLF56_01365"
                     /inference="COORDINATES: ab initio
                     prediction:GeneMarkS-2+"
                     /note="Derived by automated computational analysis using
                     gene prediction method: GeneMarkS-2+."
                     /codon_start=1
                     /transl_table=11
                     /product="hypothetical protein"
                     /protein_id="HOQ52243.1"
                     /translation="MRDIDELLLSARGVLVRKDHRAIAAQLDYAIRTGRLTTVLPGIY
                     TAPNPTWQARIRAADAFRPGGIITGAAAALALWWQDVPISAVTVAVTCRVEPHYAGFH
                     WERRMLSPDLVTERDGVRFVHPAVSVLDLIPVLGGAAIDEALRRRVVSLDELWHALRA
                     QPGRPGNLGCARLLHESRAEPWSPPSARRTCSFVPLASTAGAPTIAS"
     gene            4621..4911
                     /locus_tag="PLF56_01370"
     CDS             4621..4911
                     /locus_tag="PLF56_01370"
                     /inference="COORDINATES: protein motif:HMM:NF016370.2"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="DUF559 domain-containing protein"
                     /protein_id="HOQ52244.1"
                     /translation="MLLRAARIHGWRANHRIVIDGVAHWVDIAFPKERLVIEIDGWTY
                     HGSQASFVADRWRYARLAANGWPVLAFAASALDDEPDEFIRMVQVALARRTF"
     gene            5100..6527
                     /gene="phoA"
                     /locus_tag="PLF56_01375"
     CDS             5100..6527
                     /gene="phoA"
                     /locus_tag="PLF56_01375"
                     /EC_number="3.1.3.1"
                     /inference="COORDINATES: protein motif:HMM:NF007810.0"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_function: GO:0016791 - phosphatase activity [Evidence
                     IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="alkaline phosphatase"
                     /protein_id="HOQ52245.1"
                     /translation="MRTAPTRRMLRAGLATLAITAATGTMLAVAAPTLDLTVLHGANR
                     NDGDQTRAVRQAVIGGKARNVILLIGDGMGDSEITIARNYAKGAAGRFAGIDALPLTG
                     QYTTYSLNKATHKPDYDPDSAATGTAWATGFKSYDGAISVDVNGKAQQTLLEQAKAKG
                     LRTGNVTTAEIQDATPAVQAAHVSARSCYGPAATLKTCPEAALENGGPGSITEQIINT
                     RADVTLGGGAKTFNEVATAGAYQGKTLKQQAFERGYQIVGDKGSLDKVKRANQTQPLL
                     GLFATGNLAVRWIGPEATSDGGKKDPVRCTDNPDRSSTQPTLAQLTTKAISLLDTRRN
                     GPGFFLQVEGASIDKQDHAANACGQIGETVDLDEAVQAALAFAEKDGNTSVFVTADHA
                     HTSQIVEAGSVTPGYTVNLLTADDAPMTINYATAEKGSSMQHTGTQLRIAGYGPGAAN
                     IVGLTDQTDLNTTIRRILGLDRKRG"
     gene            6680..7531
                     /locus_tag="PLF56_01380"
     CDS             6680..7531
                     /locus_tag="PLF56_01380"
                     /inference="COORDINATES: protein motif:HMM:NF016370.2"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="DUF559 domain-containing protein"
                     /protein_id="HOQ52246.1"
                     /translation="MERIAELLAHENGVLPRRQHHTIRHQLDRARRMGLLEAPLPGIY
                     TAPDPGWHTRVRAAAAFRPGCVITGAAAAMLLWWPEVPIDAVAVAVAHDVHGTYPRYT
                     WERRRLPLDLVADRDGVRIACPAASILDLVPTLGGNVIDEGLRRRAVTLSELWSAYRA
                     QPYRPHNQLRVEMLNDSRHEPWSEAERKAHRLLRAARLTCWRTNHLIRIDGRNYFADI
                     AFPAVRVIVELDGFSHHGSKKAFVDDRWRYSRFAAHGWRVLPFAASVLDDDPQGFLTL
                     VRRAVGQ"
     gene            complement(7632..9026)
                     /locus_tag="PLF56_01385"
     CDS             complement(7632..9026)
                     /locus_tag="PLF56_01385"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_013863211.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_component: GO:0016020 - membrane [Evidence IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="tryptophan-rich sensory protein"
                     /protein_id="HOQ52247.1"
                     /translation="MKKVLVTGATGYIGSHLVPRLLDAGHQVRVLSRNPAKLPDQWRD
                     RVKVAVGDATDEADVRAALAGADVVYYLLHSMDGGGDFVARDRALAQQFARLAAEAGT
                     GRIVYLSGLHPKGELSEHLASRVEVGEILLGSGVPTAVLQAGVVLGAGSASFDMLRHL
                     TERLPAMVAPKWLENHIQPIAVKDAVFYLVAAAELPPEVNRTFDLGGPDVLTYREMMQ
                     RYAGLAGLIPRAVVTVPVLTPNLASYWVGLVTPIKSGIARPLVGSLMHDAVRDEFDAE
                     QVLGEPEGGATGFDEAVRAALGDIDPKLWGRTFGAVSAAVVGAAVVGGLFTDPNSGWY
                     RRLRKPSFQPPAAVFGPVWSLLYGLIAVSASAIIADAREAGDDAAADDFERALAANLA
                     LNTSRSGLFFRAHALKTSAVTAGLLALSSADLTRRAAASGPGKAAALGLYTAWCTFAT
                     ALSTAIAVKNRRRR"
     gene            complement(9028..9747)
                     /locus_tag="PLF56_01390"
     CDS             complement(9028..9747)
                     /locus_tag="PLF56_01390"
                     /EC_number="3.4.-.-"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_013863210.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_component: GO:0016020 - membrane [Evidence IEA];
                     GO_function: GO:0004222 - metalloendopeptidase activity
                     [Evidence IEA];
                     GO_process: GO:0071586 - CAAX-box protein processing
                     [Evidence IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="CPBP family intramembrane metalloprotease"
                     /protein_id="HOQ52248.1"
                     /translation="MPVFAELGSFFRASLLDPVQVTEKESPAKIRRRRIVVAITLLLG
                     AVALGLALAIQPGSPWFYPATLGVAAIWVAGAFASGKLHLGRARTRSGGSSYSVLQAF
                     ILGGMLLAVFLAGAVVVGRVDWLRGPVDSLLAHARYGSLPIVAVITAVNGVAEELFFR
                     GALFAAFPRRWRVVGSAALYALATVLTGVPLLVLAAAALGLLTGMQRRVTGGVLGPIV
                     THLTWSLGMLFALPWALDLWS"
     gene            complement(10093..10854)
                     /gene="otsB"
                     /locus_tag="PLF56_01395"
     CDS             complement(10093..10854)
                     /gene="otsB"
                     /locus_tag="PLF56_01395"
                     /EC_number="3.1.3.12"
                     /inference="COORDINATES: protein motif:HMM:TIGR00685.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_function: GO:0004805 - trehalose-phosphatase activity
                     [Evidence IEA];
                     GO_process: GO:0005992 - trehalose biosynthetic process
                     [Evidence IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="trehalose-phosphatase"
                     /protein_id="HOQ52249.1"
                     /translation="MSSLDELARTPRLLVALDVDGTLAPLLDRPLEVRLTPQARAAVN
                     RLAGLPQTFVALVSGRTLEHLRIIGEHADDSPLLLAGSHGAEYWVPGEGEQQRSDDPA
                     EVELRTRLLARAEVLVEPYEGAWIEPKTFGLAVHTRLSSEADGAKANKAVDELVVAEA
                     PGWRRRTGRDIVEYAFREEGKDSAVRTLRERTGATAVLFAGDDVTDEDALGSLEPQDV
                     GVRVGEGPTHASLRVPDVAGMAELLGRLAELRARG"
     gene            complement(10851..>11582)
                     /locus_tag="PLF56_01400"
     CDS             complement(10851..>11582)
                     /locus_tag="PLF56_01400"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_011757421.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_function: GO:0003824 - catalytic activity [Evidence
                     IEA];
                     GO_process: GO:0005992 - trehalose biosynthetic process
                     [Evidence IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="trehalose-6-phosphate synthase"
                     /protein_id="HOQ52250.1"
                     /translation="RALAKAFPISIDVESYEQLAASDEVKARAKEIRDGLGNPKKVLL
                     GVDRLDYTKGIRHRLKAFEELLADGRLTVEDVTLVQVASPSRERVETYMQLRDEIELA
                     VGRIDGDFDTMGHSAVRYLHQAFPREEMVALYLAADVMLVTALRDGMNLVAKEYVASR
                     LDNRGVLVLSEFAGAHDELGSAVTVNPHDIDGVKDAIMRAVEMKPGEQGRRMRALRKK
                     VREHDVEDWSQDFLAALADAKERAK"
BASE COUNT         1844 a         4141 c         3941 g         1656 t
ORIGIN      
        1 cccgagagcc gcgcctacga ccgaacgctg gtgctgcttt acatcggcac cctggcggtc
       61 aactcgctcc ttctctacgt gatgagtgtc gtggtgcgac gaacacctgc cacctggagg
      121 ccggacgatc ctccgaccag gcggatgggc gaggcggtca acctgtccat gacgatcctc
      181 ttcagtctcg cggccgtgct cgcgttgctg tttccggcga tcaacatggc gacgatgttg
      241 ctgctgttct tcaccggacc gctcgcgaac ctgttgctgc ggcgcagtac gccgccgagc
      301 gctccctagt tcctgggcgg tggtctaggg caggctaggg cgggtcacca acctgacccg
      361 cagcgtcgcc agaggcgtac cggggtagtg cacccagcgc cggtatcgcg ccccacctag
      421 tctgatccca tgctgaccgc attgcatgca ttgatccatt ccgacgaccc gacggccacc
      481 cgggcgttcc tccgcgatgt gctgcgctgg ccgttcgtct ccgagggagc cgagggcgac
      541 accggcgtcg gcggcgccgg caccggagga accgacgccg ccgaatggct gatcctccgc
      601 agcgggccca gcgaggtggc ggttcatccg accacgtggc aggccgggga cgagccgcgg
      661 tcggttcccc ggcagcacac ggtggccctc atctgtgacg acctggcgac gaccatggac
      721 gaactgaggg ggcgcggggc tgagttcagc accgcaccgc gtgacgccgg ctacggcgtc
      781 gcggtgatgc tgaaggtgcc gggtgcggac gacctgctgc tgtatcaggc cgcgcacgcg
      841 gtggcgtatt cgttgcccga tgacgccggc caccagcagc gctcggccgg gcctggcagc
      901 ggtgggcacg aacacggtga ccaacagtct ggcgagcagg ggcaggggca tcagtccggc
      961 gagcaggggc accggcacgg cgagcagggg caccggcacc gcgagcacga gcacgagcac
     1021 gagcacgagc acggcgagca ccagcacggt ggccggcgga tgccggtcac cgacgacgaa
     1081 tggcatcagc actacgcacc cgaagcgatc tggagtggca accccaacgg cgcgttggtg
     1141 gccgagttgg ccggcctcac gcccggtcgg gccgtcgacg tcgggtgcgg cgagggtgcg
     1201 gacgcggtgt ggctggccgg ccagggttgg caggtgaccg cgttcgacgt cgccgagagc
     1261 gcgctggaac gcggccgggc cgccgccgag tcggccgggg tcgaggtcga gtgggtgcgg
     1321 gccgggctgc tgtcgtccga actgcaggcg ggtgcgttcg acctggtcag cgcctgttat
     1381 ccggcgctgc agcgcactcc ggagcggctc gccgaacagg cgctgcttcg cctggtcgcg
     1441 ccgggtggac ggctcctgat gctcgcgcac gccgacgtcg accgcaccac ggccctcgag
     1501 cacggcttcg atcccgacga ctacctggac cccggtgacc tgctcaccgc ggcgtccgac
     1561 gggtggagcg tggagcggca cgagcggcgt ccacgacgca tcgacggtgg tgcgggggcg
     1621 ggtcaccaca ccgacatcgt gttggtgctg cgacgggagc agagcgcatg agtgggcaag
     1681 gcgtggcgct ggtcaccgga gcatcgtccg gcatcggtgc cgagagcgcg aaacacctgg
     1741 ccgcagcggg tttcctggtc tatgcggcgg cccggcgtac tgaccgactg ggcgagttgg
     1801 cgtccggcag catccgtccg ctgacgatgg acgtgaccga cgacgactcg gtcaccgccg
     1861 gcgtgcagca ggtgctggct gaggccgggc gcatcgacgc gctggtcaac aatgcggggt
     1921 acgggtcgta cggcgccgtc gaggacgttc cgctcgacga ggcccgacgc cagttcgagg
     1981 tgaacctgtt cggcctggcc cggctcaccc agctggtcat cccggggctg cgccagcagg
     2041 gttcgggacg tatcgtcaac atcagttcta tgggcggtcg gttcgcgatg ccgttcggcg
     2101 cctggtacca cgccaccaag ttcgcggtcg agggcttcag cgacgccctg cgccaggagt
     2161 tggcaccgtt cggcatccag gtggtggtga tcgagcccgg cggcatcaag accgaatggg
     2221 gcggcatctc ggcggcctcg gccctggagc gctccggcga gggtccctac gccgctcggg
     2281 tgcagcggat cgcgcgcgcc ctggccggca gcgaccggcg gttcggctcc gaacccggtg
     2341 tggtggcgaa ggcgatcacc actgctgtga ccgccaggcg tccccgcacc cgctacgcca
     2401 tcggcgccgg cgccaagccg gcgatctacg cccggcggtt tttgcccgac cgggtcatgg
     2461 acgcgctgat cggccgcgtg ctcggttgac catggccgat cgcacggtga gaccctccga
     2521 ctaccgccga caacccccgg gcgcaccgat cggtttgtca tgatgggcgg catgtccagc
     2581 agtgccgaga gcaggcgcgc cgtccgtcca gatgagccgg acgagccgcg catcgagcat
     2641 gtcgggcacg tctggcgggt gcggtcggca gcggcggctc ggcaggtcct gcgcgagcgg
     2701 cgcaccacca cgcaggcggg gttcaccgcc gaggccattc cgaaggggta cctgaagcat
     2761 catccgatcc tgatgtcgga cggcccgttg cacgacgagc agcgcaccaa ggtcggacgg
     2821 tttttcgcgc cgaaggtggt ggaggagcgg tacctggacc tgatggagtc gtgcgccgac
     2881 cggttgctcg gcgacgcggc ggcgtccggg atggtgcggc tggacgatct cgcgctgctc
     2941 tacacagtcg aggtgacggc gcaggcggtg gggctgaccg agtcgtcggt gccggcgatg
     3001 gcgaagcggc tggtgacctt cttcaatcag ccgccgttcg acatcacccg gcccgatctc
     3061 ggccgcacgc cccggcagtg ggcgcaggcg gcgctgaacg gactgctgcc gatcgggcgg
     3121 ttctggtggt cggacgtgcg tccggcgatc cgggtgcggc gccggaagcc gcgtgaggac
     3181 gtgatcagcc acctgatcgc cgagggctac acgaacgccg acatcctggt cgaatgcgtc
     3241 acctacggca cggccggcat ggtgaccacc cgagagttca tcgcgatggc cgcctggcac
     3301 ctgctgggca acgagccgtt gcgggcgcgt tacctggtgg ccgggcaggc cgagcggctg
     3361 gcgatcctga acgagatcat ccggctcgaa ccggtggtcg ggcacctgta ccggcgggtg
     3421 cagcagccgg tcacgttcac cgatcagggg cacgagcaca ccgcccagcc gggtgacctg
     3481 atcgatctgt gcatccgtcc ggccaacgca gacccagaga cggtgggtga gagacccctg
     3541 caactgtgcc cggggcgtcc attgcccaag ggcgtgaacg aggcggtgct cagcttcggc
     3601 gatggggcgc acaagtgccc gggtcagccg ctggcgatgc tggagtcgga cgccctgctg
     3661 cgccggctgc tggcccgtga ggtcagcatc gtgaccgaac ccagtatcga gtgggacgac
     3721 ctgatcgccg gctacgccct gcgcggcttc gacatgcaga tcggcgacgg ctgattcgtc
     3781 ggtcgagacg cagacgcctc gagatccgag cgaaggttca ccggcgaggg ccgaggtggt
     3841 cctcggctga agcggtccgg aggcagcccc gggggccgca agaaggttca tcgggtgggt
     3901 gccgcctcgt cggtttctga gatcacccgc acgggttgcg aaggtcttcg gcgtgtcgcg
     3961 gggtgagggt cggactggtg gcgagatctc agaatgatca cgatctgccc gctcactcac
     4021 aaacagggca ctcaacagtg cattcaaggg tatgagagac attgacgaac tgctgttgtc
     4081 ggcccgtggt gtgctggtgc gcaaagacca ccgcgcgatc gcggcccaac tcgactatgc
     4141 gatccgcacc ggccggctca ccaccgtcct acctggcatc tacaccgccc cgaatccaac
     4201 ctggcaagcg cgaatacgag ccgcagacgc cttccgtccg ggtggcatca tcaccggcgc
     4261 ggcggccgcg ctggcgctgt ggtggcagga cgttccgatc agcgccgtca cggtggccgt
     4321 cacgtgcagg gttgagccgc actacgcggg ctttcactgg gagcgccgga tgctctcgcc
     4381 cgacctggtc acggaacgtg acggggtgcg tttcgttcat ccggcggtgt cggtgctgga
     4441 cctgatcccg gtgctgggcg gtgctgcgat cgacgaggcg ctgcgccgtc gcgtggtgag
     4501 tctggacgag ttgtggcacg cgttgcgcgc acagcccggg cgccccggca acctgggctg
     4561 cgcccggttg ctgcacgagt cgcgtgccga gccctggtca cccccgagcg caaggcgcac
     4621 gtgctccttc gtgccgctcg catccacggc tggcgcgcca accatcgcat cgtgatcgac
     4681 ggcgtcgcgc actgggtcga catcgcgttc cccaaggagc gcttggtgat cgagatcgac
     4741 ggctggacct accacggcag ccaggcctca ttcgtcgccg accggtggcg gtacgcccgg
     4801 ctcgcggcca acggctggcc ggtgctggcc ttcgccgcct cggcgctcga cgatgaaccc
     4861 gacgagttca ttcggatggt acaggtggcg ctggcccgcc ggaccttctg aaatcccgac
     4921 accagacgag acctcgaccg gcgacacgcc gtggttcctc gcgaccactg cgggtgatct
     4981 cagaatcaac caaccgaccg gccaccgaag cgtcgcccat ccgtcacccg cacgcctccc
     5041 cccggacgcc cggcgctgct ggactccgca tgtccaaccc cacccacgaa gggaacccca
     5101 tgcgaaccgc cccgacccga cgaatgctgc gcgccggcct tgccaccctc gccattacgg
     5161 cggccaccgg cacgatgctc gccgtcgccg cccctacgct cgacctgacc gttctgcatg
     5221 gcgccaaccg caacgacggc gaccagaccc gcgcggtgcg gcaggccgtg atcggcggca
     5281 aggcacgcaa cgtgattctg ctgatcggcg acggcatggg cgactccgaa atcaccatcg
     5341 cgcgcaacta cgccaagggt gccgccggac gcttcgccgg catcgatgcc ctgccgctga
     5401 ccgggcagta caccacctat tcgctgaaca aggccaccca caagcccgac tacgaccccg
     5461 actcggccgc caccggcacc gcctgggcga ccggcttcaa gtcctacgac ggcgccatct
     5521 cggtcgacgt caacggcaaa gcccagcaga cgctgctcga gcaggccaag gccaagggcc
     5581 tgcgcaccgg caacgtgacc accgccgaga tccaggacgc cacaccggcc gtgcaggccg
     5641 cgcacgtgtc ggcacgctcg tgctacggcc ccgccgcaac tctcaagacc tgccccgagg
     5701 ccgccctcga gaacggcgga ccgggctcga tcaccgagca gatcatcaac acccgcgccg
     5761 acgtcaccct cggtggcggt gccaagacct tcaacgaggt ggccacggcc ggcgcctatc
     5821 agggcaagac gctcaagcag caggccttcg agcgcgggta ccagatcgtc ggtgacaagg
     5881 gcagcctcga caaggtgaag cgggcgaacc agacccagcc gctgctcggg ctgttcgcca
     5941 ccggcaacct ggcggtgcgc tggatcggcc ccgaggccac ctcggacggc ggcaagaagg
     6001 atcccgtgcg ctgcaccgac aacccggatc ggtcgagcac ccagcccacg ctggcccagc
     6061 tcaccaccaa ggcgatcagc ctgctcgaca ccaggcgcaa cggcccgggc ttcttcctgc
     6121 aggtggaggg tgccagcatc gacaagcagg atcacgccgc caacgcctgc gggcagatcg
     6181 gtgagaccgt cgatctggac gaagccgtgc aggccgccct ggccttcgcc gagaaggacg
     6241 gcaacacctc ggtgttcgtc accgccgacc acgcccacac cagccagatc gtcgaggccg
     6301 gctcggtcac ccccggctac accgtcaacc tgctgaccgc cgacgatgcg ccgatgacga
     6361 tcaactacgc cacagccgag aagggcagct cgatgcagca caccggcacc cagctgcgca
     6421 tcgcgggcta cggtcccggt gcggcgaaca tcgtggggct caccgatcag accgacctga
     6481 acaccactat ccggaggatc ctcggcctgg accgcaagcg cggctgaccc aaccaccgcg
     6541 gctcgcgagg gctcgcggaa acgagacctg cgcgtcagtg agcggcgtga cggcgtgtcc
     6601 gcacgtccga gcggcactgg cgcgcaggtt tcggtcattc ccactcacaa aaggggggtc
     6661 gaaaagtgca ttcataggta tggaacgcat tgctgaactc ctcgcccacg aaaacggggt
     6721 gctgccgcgc cggcagcacc acacgattcg ccaccaactc gatcgcgccc gtcgcatggg
     6781 cctgctggag gcacccctgc ccggcatcta cacggcgccc gacccgggct ggcacaccag
     6841 ggttcgcgcc gcggcggcct tccgacccgg atgcgtcatc accggtgccg cggcggcgat
     6901 gctgctgtgg tggcccgagg tgcccatcga tgcggtggcg gtggcggtgg cgcacgacgt
     6961 gcacgggacc tatccccggt acacctggga gcggcgcagg ctaccactcg acctggtcgc
     7021 ggaccgcgac ggggtgagga tcgcctgccc agccgcctca atactcgatc tcgtgcccac
     7081 cctcggcggc aacgtgatcg acgaggggct gcgtcgccgc gcggtgacgc tgagcgaact
     7141 ctggtcggcg taccgggctc agccgtaccg cccccacaac cagttgcggg tcgagatgct
     7201 gaatgactcc aggcacgagc cctggtccga ggctgagcgg aaagcgcacc ggctactccg
     7261 cgcggcccgg ctgacctgtt ggaggaccaa ccacctcatt cgcatcgacg gacgcaacta
     7321 cttcgccgac atcgccttcc cggccgtgcg ggtgatcgtg gaactggatg gcttcagcca
     7381 ccacggcagt aagaaggcgt tcgtcgacga ccgctggcgc tattcgaggt tcgctgcgca
     7441 cggctggcgg gtgctgccgt tcgcggcctc ggtgctggac gacgatcccc aaggcttcct
     7501 gaccctggtg cgccgggctg tgggtcagta gcgattcacc gaaaggtgcg cgccagtgcg
     7561 gcgccgacgg cacgacacgc cgtcacctgc tgcactggcg cgcaggtttc gtcttgtcgg
     7621 gcgcggacct gtcagcgccg ccggcggttc ttgacagcga tcgcggtgct gagcgccgtg
     7681 gcgaaggtgc accaggcggt gtacaggccg agggccgccg ccttgccggg gccactggct
     7741 gcggcacgcc gggtcagatc agcgctgctg agcgccagca gccccgccgt caccgccgat
     7801 gtcttcagcg cgtgggcacg gaagaacaac ccgctccgcg aggtgttcag cgccaggttg
     7861 gcggccaggg cccgctcgaa gtcgtccgct gccgcgtcgt ccccggcctc gcgagcatcg
     7921 gcgatgatgg cgctcgccga gaccgcgatc aggccgtaca gcagcgacca gaccggcccg
     7981 aacacggccg ccggcggctg gaacgacggc ttgcgcagcc gccggtacca cccgctgttc
     8041 gggtcggtga acagcccgcc gaccaccgcg gcgcccacca ccgcggccga caccgcgccg
     8101 aacgtgcggc cccacagttt cgggtcgatg tcgcccagag cggcccgaac cgcctcatcg
     8161 aagccggtcg caccgccctc gggctcaccc agaacctgtt cggcgtcgaa ctcgtcacgg
     8221 acggcgtcgt gcatcaggct gccgaccaac ggacgggcga tgcccgactt gatcggcgtg
     8281 accaggccga cccagtagct ggccaggttc ggggtcagca ccggcacggt cacgacggct
     8341 cgcggaatca gtcccgccag cccggcgtag cgctgcatca tctcgcggta ggtgagcacg
     8401 tcggggccac ccagatcgaa ggtgcggttc acctccggcg gcagttcggc cgccgcgacg
     8461 agatagaaca ctgcgtcctt caccgcgatc ggctgaatgt ggttctccag ccatttcggc
     8521 gccaccatcg ccggcagccg ctcggtcagg tgccgcagca tgtcgaagga cgccgacccg
     8581 gctccgagca ccacgcccgc ctgcagtacg gcggtgggca cccccgagcc cagcaggatc
     8641 tcacccacct cgacccggga cgccaagtgc tccgacagct cgcccttcgg atgcagtccg
     8701 gagaggtaga cgatgcgccc ggtgcccgcc tcggccgcga gcctcgcgaa ctgctgcgcc
     8761 agggcacggt cgcgggccac gaagtcgccg ccgccgtcca tcgagtgcag caggtagtag
     8821 accacgtccg caccggccag ggccgcacgg acgtccgcct cgtcggtggc gtcgcccacg
     8881 gcaaccttga cgcggtcgcg ccactggtcg ggcagctttg ccgggttgcg ggacagtacc
     8941 cgcacctggt ggccggcgtc cagcaaccgg ggcacgaggt gcgatccgat gtagccggtc
     9001 gcgccggtga cgagaacctt cttcatgtca gctccagaga tcgagggccc agggcagggc
     9061 gaacagcatg cccagggacc aggtgaggtg ggtcacgatg gggccgagca cgccgccggt
     9121 gacccggcgt tgcatgcccg tgagcaggcc gagggcggcg gcggccagca ccagcagggg
     9181 cactccggtc agcaccgtgg ccagagcgta gagagcggct gagccgacca cccgccagcg
     9241 ccgcggaaag gccgcgaaca atgcaccgcg gaagaacagc tcctcggcga ccccgttcac
     9301 cgcggtgatc acggcgacga tcggcagcga gccgtaccgg gcgtgtgcga gcagcgagtc
     9361 gacgggaccg cgcagccagt ccacccgtcc gacgacgacc gccccggcca ggaacaccgc
     9421 cagcagcatg ccgccgagga tgaacgcctg cagcaccgag tagctcgagc cgcccgaacg
     9481 ggtgcgggcg cggcccagat gcagcttgcc ggacgcgaat gcgccggcca cccagatcgc
     9541 ggcgaccccg agcgtggccg gatagaacca ggggctgccg ggctggatcg cgagcgccag
     9601 gccgagcgcc accgctccca ggagcaaggt gattgcgacc acgatccggc ggcgacggat
     9661 cttcgcgggt gactccttct cggtgacctg gacgggatcg agcaggctgg cgcggaagaa
     9721 cgaccccaac tccgcgaata ccggcacccc ctaagcctag ggaggcacac ccaacacgtc
     9781 cccacccagg cggggggtca gtccctgatg acgacccgcc gatcgaaggg accggcaccc
     9841 cgttggtcga gcctgtcagg accagccggg ccgacccccg tcggtcgagc ccgtcgaccg
     9901 caccaacctc gatctcccag gtcgagcctg tcgaccgcac caacctcgat ctgccgggtc
     9961 gagcctgtcg accgcaccaa cctcgatctc cccggtcgtg agtgtcgata ccgcacgcaa
    10021 cgtcgacccc gtcggtcgag cctgtcgaga cgcctcgccc gtcacatctc acagccatcg
    10081 acgcccgact gctcagcccc gtgcgcgcag ctcggccagc cgtcccagca actccgccat
    10141 cccagcgacg tcgggaaccc gcaggctcgc gtgcgtgggg ccctcgccca cccggacgcc
    10201 gacgtcctgc ggttcgaggc tgccgagcgc gtcctcgtcg gtcacgtcgt cgccggcgaa
    10261 gagcaccgct gtggccccgg tgcgctcccg gagcgtccgc accgccgaat ccttgccctc
    10321 ctcgcggaag gcgtactcga cgatgtcgcg cccggtgcgt cgccgccagc cgggcgcctc
    10381 ggcgacgacc agctcgtcga ccgccttgtt cgccttggcg ccgtccgcct cggacgacag
    10441 ccgggtgtgc accgccaaac cgaaggtctt cggctcgatc caggcaccct cgtacggttc
    10501 gaccagcacc tcggcccgcg cgagcagtcg cgtgcgcagc tcgacctcgg cggggtcgtc
    10561 ggagcgttgc tgctcaccct cgccgggtac ccagtactcc gcgccgtgcg atcccgccag
    10621 cagcagcggg gagtcgtccg catgctcgcc gatgatgcgc aggtgctcca gcgtgcggcc
    10681 cgaaaccaag gccacgaacg tctgcggcaa ccccgccagc cggttcaccg cggcccgcgc
    10741 ctgcggggtc agccggacct ccagcggccg gtccagcagc ggcgccagcg tgccgtccac
    10801 atcgagtgcg accagcaacc gcggcgtgcg cgccagctcg tccaggctgc tcatttcgcg
    10861 cgttcctttg cgtcggccag cgcggccagg aagtcctgtg accagtcctc gacgtcgtgc
    10921 tcgcgcacct tcttgcgcag cgcccgcatt cgccggccct gctcacccgg cttcatctcg
    10981 accgcccgca tgatcgcgtc cttgacgcca tcgatgtcgt ggggattgac cgtcaccgca
    11041 ctgccgagct cgtcgtgcgc gccggcgaac tcgctcagca ccagcacgcc gcggttgtcc
    11101 agccggctgg ccacgtactc cttcgcgacc aggttcatgc cgtcgcgcag ggcggtgacc
    11161 agcatcacgt ccgcggccag gtagagcgcc accatctcct cgcgcggaaa agcctgatgc
    11221 aggtagcgga cggcgctgtg ccccatggtg tcgaaatctc cgtcgatgcg gccgacggcc
    11281 agttcgatct cgtcgcgcag ctgcatgtag gtctccaccc gctcgcggct cgggctggcc
    11341 acctgcacca gggtgacgtc ctcgaccgtc agccggccat cggccagcag ctcctcgaac
    11401 gccttcaacc ggtgccggat gcccttggtg tagtccagcc ggtcgacgcc cagcagcacc
    11461 ttcttcggat tgcccaagcc gtcgcggatc tcctttgccc gcgccttcac ctcgtcgctg
    11521 gcggccagtt gctcgtagga ctcgacgtcg atcgagatcg ggaaggcctt ggccagcgca
    11581 cg
//
LOCUS       DAOSUG010000021        11575 bp    DNA     linear   ENV 10-JUN-2024
DEFINITION  MAG TPA_asm: Micropruina sp. isolate AMR_MDS_2195
            NODE_3163_length_11575_cov_4.755469, whole genome shotgun sequence.
ACCESSION   DAOSUG010000021 DAOSUG010000000
VERSION     DAOSUG010000021.1
DBLINK      BioProject: PRJNA925477
            BioSample: SAMN32864262
            Sequence Read Archive: SRR4236673
KEYWORDS    WGS; ENV; Metagenome Assembled Genome; MAG; Third Party Data; TPA;
            TPA:assembly.
SOURCE      Micropruina sp. (wastewater metagenome)
  ORGANISM  Micropruina sp.
            Bacteria; Bacillati; Actinomycetota; Actinomycetes;
            Propionibacteriales; Nocardioidaceae; Micropruina.
REFERENCE   1  (bases 1 to 11575)
  AUTHORS   Abdulkadir,N., Saraiva,J.P., Zhang,J., Stolte,S., Gillor,O.,
            Harms,H. and Rocha,U.
  TITLE     Genome-centric analyses of 165 metagenomes show that mobile genetic
            elements are crucial for the transmission of antimicrobial
            resistance genes to pathogens in activated sludge and wastewater
  JOURNAL   Microbiol Spectr 12 (3), e0291823 (2024)
   PUBMED   38289113
REFERENCE   2  (bases 1 to 11575)
  AUTHORS   Abdulkadir,N.
  TITLE     Direct Submission
  JOURNAL   Submitted (24-MAR-2023) Environmental Microbiology, Helmholtz
            Center for Environmental Research -UFZ, Permoserstr 15, Leipzig
            04318, Germany
COMMENT     The annotation was added by the NCBI Prokaryotic Genome Annotation
            Pipeline (PGAP). Information about PGAP can be found here:
            https://www.ncbi.nlm.nih.gov/genome/annotation_prok/
            
            ##Genome-Assembly-Data-START##
            Assembly Method        :: SPAdes v. 3.11.1
            Genome Representation  :: Full
            Expected Final Version :: Yes
            Genome Coverage        :: Not Applicable
            Sequencing Technology  :: Illumina MiSeq
            ##Genome-Assembly-Data-END##
            
            ##Genome-Annotation-Data-START##
            Annotation Provider               :: NCBI
            Annotation Date                   :: 05/04/2023 04:57:10
            Annotation Pipeline               :: NCBI Prokaryotic Genome
                                                 Annotation Pipeline (PGAP)
            Annotation Method                 :: Best-placed reference protein
                                                 set; GeneMarkS-2+
            Annotation Software revision      :: 6.5
            Features Annotated                :: Gene; CDS; rRNA; tRNA; ncRNA
            Genes (total)                     :: 2,776
            CDSs (total)                      :: 2,750
            Genes (coding)                    :: 2,714
            CDSs (with protein)               :: 2,714
            Genes (RNA)                       :: 26
            tRNAs                             :: 24
            ncRNAs                            :: 2
            Pseudo Genes (total)              :: 36
            CDSs (without protein)            :: 36
            Pseudo Genes (ambiguous residues) :: 0 of 36
            Pseudo Genes (frameshifted)       :: 2 of 36
            Pseudo Genes (incomplete)         :: 32 of 36
            Pseudo Genes (internal stop)      :: 2 of 36
            ##Genome-Annotation-Data-END##
FEATURES             Location/Qualifiers
     source          1..11575
                     /organism="Micropruina sp."
                     /mol_type="genomic DNA"
                     /submitter_seqid="NODE_3163_length_11575_cov_4.755469"
                     /isolate="AMR_MDS_2195"
                     /isolation_source="Activated sludge of municipal
                     wastewater treatment plants"
                     /db_xref="taxon:2737536"
                     /environmental_sample
                     /geo_loc_name="Canada"
                     /lat_lon="49.25 N 123.1 W"
                     /collection_date="2015-06-16"
                     /metagenome_source="wastewater metagenome"
                     /note="metagenomic"
     gene            <1..267
                     /locus_tag="PLF56_01405"
     CDS             <1..267
                     /locus_tag="PLF56_01405"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_013864951.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_component: GO:0016020 - membrane [Evidence IEA];
                     GO_function: GO:0015267 - channel activity [Evidence IEA];
                     GO_process: GO:0055085 - transmembrane transport [Evidence
                     IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="aquaporin"
                     /protein_id="HOQ52251.1"
                     /translation="DSATKTPAGLGWLGALGVALLVVGIGASLGGPTGYAINPARDLG
                     PRIAHAVLPIPNKGSSDWGYSWIPVVGPIIGGVAAGLLSHSLLP"
     gene            374..1891
                     /gene="glpK"
                     /locus_tag="PLF56_01410"
     CDS             374..1891
                     /gene="glpK"
                     /locus_tag="PLF56_01410"
                     /EC_number="2.7.1.30"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_014847411.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_function: GO:0004370 - glycerol kinase activity
                     [Evidence IEA];
                     GO_process: GO:0006071 - glycerol metabolic process
                     [Evidence IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="glycerol kinase GlpK"
                     /protein_id="HOQ52252.1"
                     /translation="MAEKYVLAIDQGTTSSRAIVFNHAGRIVGTGQKEHEQIFPKAGW
                     VEHNAIEIWDNIREVVGIALADASVNKDNLVAVGITNQRETALVWDKTTGEPVYNAIV
                     WQDTRTNKIIEQLGGGDQDKYKDRVGLPLATYFSGPKVKWILDNVEGAREKAEAGDLV
                     MGNMDTWVIWNLTGGTDGGVHVTDVTNASRTMLMDLKSLSWDESIAADMGIPMSMLPE
                     IKSSSEVYGHGREAGLLGGVPIAGDLGDQQAATFGQAAFEVGMAKNTYGTGCFMIMNT
                     GEELVPSKNGLLTTVCYKIGDNKPIYALEGSIAVTGSLVQWLRDNLRMFDSAPEVEQL
                     ATQVEDNGGVYVVPAFSGLFAPYWKSDARGAIVGLTRYVNRNHIARAVLEATAFQTRE
                     VLDAMEADSGVHLAELKVDGGMTMNDTLMQFQADQVGVPVVRPVVAETTALGAAYAAG
                     IAVGFWNGEQDVIDNWAEDKRWEPSMDEGERERLYRQWKKAVTRTFDWVDDDVKE"
     gene            2108..3433
                     /locus_tag="PLF56_01415"
     CDS             2108..3433
                     /locus_tag="PLF56_01415"
                     /inference="COORDINATES: protein motif:HMM:NF024883.2"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_function: GO:0005515 - protein binding [Evidence IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="GAF domain-containing protein"
                     /protein_id="HOQ52253.1"
                     /translation="MLERILRSAVELLHSASGSLCLVDEQAHTYHKEIDLDAGCETGR
                     PLSLDEGMTGLVVRSRGPVILEQYAQVPRGHLAAGEPRYHRAVIGAPIMLGDQLIGAL
                     VVFAGDGQRYGSDDARLLQEFAGHAAIAIANSRVYAMAEERAKQVAVAAERERSMQEV
                     HDTFGRGLATVMLRLQAAQRAAASGSGVADQLAAAQAAVDDALREGRRAVWGTGSLGI
                     GDTRTLDEAIRLELDWAAATANLNTNFRVFGDRGEIAPEVGMQLLRIVQEALTNVAQH
                     ARASAVRIGLVFGTDGVAVIVEDDGVGFDVEAVRQRRTGMGLAGLVSRATQVGGRVQL
                     ESTPGWGTRIRADLPYRPTAGERDGVSRWRVVIVHELPAMRAGLVQLLDESEPGVQVV
                     AEVCDSHRSVAVFCTFGGGPLRPLLDHDARWVDNPAERAHPEPTLDRGF"
     gene            3957..4349
                     /locus_tag="PLF56_01420"
     CDS             3957..4349
                     /locus_tag="PLF56_01420"
                     /inference="COORDINATES: ab initio
                     prediction:GeneMarkS-2+"
                     /note="Derived by automated computational analysis using
                     gene prediction method: GeneMarkS-2+."
                     /codon_start=1
                     /transl_table=11
                     /product="hypothetical protein"
                     /protein_id="HOQ52254.1"
                     /translation="MQAVVQCLVKKGVSARFEVAEDGTPRVAMPATTKAQIDRNNAAQ
                     EECRKELPPTPEPTTDADFREFYAHLNAEAACVRQEGYETAPAPSFQKFLESARGTGI
                     DWDPFRDVPPQLVDSVRAKCQTDPNKWW"
     gene            complement(4393..5496)
                     /locus_tag="PLF56_01425"
     CDS             complement(4393..5496)
                     /locus_tag="PLF56_01425"
                     /inference="COORDINATES: ab initio
                     prediction:GeneMarkS-2+"
                     /note="Derived by automated computational analysis using
                     gene prediction method: GeneMarkS-2+.
                     GO_function: GO:0042626 - ATPase-coupled transmembrane
                     transporter activity [Evidence IEA];
                     GO_function: GO:0140359 - ABC-type transporter activity
                     [Evidence IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="ABC transporter permease"
                     /protein_id="HOQ52255.1"
                     /translation="MTRTERWRKVLAEAWAMALSQRVASILTLVLVGGMGATVLLTAG
                     RTAAAERAILASIDDAGSRAVVVRAEPKAGLDTTVVARLNTIEDAEWVGAFGAAQDAT
                     NGGTGGDVSVAVRALYATDLTPLHISPTNAEGCYASTQALTVLGMPDAAGYLASDGTP
                     VCDVTGRLTTPAFLAQFEPLVVLPQPSTAEQEPQVAIVVVLARHPEQVAAVAAATTAL
                     LSPLDASAVSVTASPDIARLRALVQGQLGGFGRSLTLTLTTVLAALVGALLFGLVMIR
                     RKDFGRRRALGATRSLIVALVTISTLYVAVAASLLGGTIAMAALVAGGEPLPGWDFIA
                     ATLILTSAAAGTGAIPPSIVAARRDPLTELRVP"
     gene            complement(5496..6134)
                     /locus_tag="PLF56_01430"
     CDS             complement(5496..6134)
                     /locus_tag="PLF56_01430"
                     /inference="COORDINATES: protein motif:HMM:NF012235.2"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_function: GO:0005524 - ATP binding [Evidence IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="ATP-binding cassette domain-containing protein"
                     /protein_id="HOQ52256.1"
                     /translation="MTAFGCEQLEFGYENLPSILTGVSLSAEAGELVAITGPSGRGKS
                     TLLYLLGLMLSPRAGRVLVKGEPVSGLPDAQRSALRARLLGFVFQDAALDQTRTVIDN
                     ITEVCLYSDLDRQTARERGLALIEQLGVQVPAERRPGQISGGQAQRIALCRALVHDPM
                     VLLADEPTGNLDDASADGVIDAMRRHSQTGRAVIVVTHDPRLERACDRSVPL"
     gene            complement(6131..7138)
                     /locus_tag="PLF56_01435"
     CDS             complement(6131..7138)
                     /locus_tag="PLF56_01435"
                     /inference="COORDINATES: protein motif:HMM:NF013625.2"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="biotin/lipoyl-binding protein"
                     /protein_id="HOQ52257.1"
                     /translation="MAVLLVGGVIGWAVAVATRPPQQVAAESTYVLVEAKEGAIGQSV
                     AAVVTASWPLRQVAVNQASGTITRLLVSRGEDVEAGDVLYRVDERAVVALPGAVPAFR
                     ALTLGVSGADVTQLTRFLVAKKFLASTTSTFTASVQRAVRSWQKSLGVAGTGRLDRGD
                     VLFVKTLPVRVALSSKFAVGGQVSAGDPAIEALASAPRFTVQYSDLQAKQVTEGMAIT
                     VDLGTQRWKGSVGPLTVTQDGSVKAPVRAAEGRTLCGEQCGKVPLQGTTQLKGTTEVQ
                     QSVAGVVVPLAALVSDANGSPGVIAADGQRVTVKVVARARGMAVVEGLAAGTAVRVPG
                     Q"
     gene            7548..9281
                     /locus_tag="PLF56_01440"
     CDS             7548..9281
                     /locus_tag="PLF56_01440"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_013161950.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_process: GO:0015074 - DNA integration [Evidence IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="DDE-type integrase/transposase/recombinase"
                     /protein_id="HOQ52258.1"
                     /translation="MHDPLLFHCRVHEEAGQDDRVPERGLLTVPDEVWALAVRRAKVI
                     GPLAAAGTAGEEAVAAAAGQLGVSRRQVYVLLRRWREGQGVVSDLIPGRSSGGRGGQR
                     LAVEVEEVIREVLRKHYLTRQRKTVAAVHREVRRVCRVRGLPVPSRGAIVRRIAGLDP
                     HAEKTAREGADAARALESAGGQVPPVIGVLEQVQIDHTVVDLIVVDERHRLPIGRPYV
                     TVGIDVYSRCIVGLVTTLEAPSALSVGLCLAHMVTDKRAWLERLGVEAAWPMAGKPGE
                     LYLDNAAEFKSEALRRGCEEHGITLRYRPPGRPHFGGIVERVIGTLMEMVHELPGTTF
                     SNPAQRGSYDSEARATLTVAELEKWLAVAVASYHGQVHGTTRQTPQARWAAGIEQRSP
                     TMVGDETAFLVDFLPVIRRTLTRTGFVIDHVHYFSDALKPWIARRGRLDRFVIRRDPR
                     DISRIWVLDPDGGSYLTVPYRTLSHPPVSVWEHRTAVERLREEGRAQVDEEGLFRMVE
                     QMRTLIDTAAATTRKARRDAERRSKAGTAGPRDKPALAAPPDDTLADGTSSSRAADAG
                     SVPPFEVIEQW"
     gene            9278..10210
                     /locus_tag="PLF56_01445"
     CDS             9278..10210
                     /locus_tag="PLF56_01445"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_003155746.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="TniB family NTP-binding protein"
                     /protein_id="HOQ52259.1"
                     /translation="MNGGGSWVGEDHQGDALDHLHVSAQAVARLPAEERLRHVRADRW
                     IGYSRATAALERLDTLYAWPGKQRMPNLLLIGPTNNGKSMIIEKFRRLHPPTSHADRE
                     QMPVLAVQMPSDPSVIRFYVALLAALGAPLRPRQRLAELEQATLTLLRAVGVRVLVID
                     ELHNLLAGRGETRREFLNLIRFLGNELRIPLVGVGTREAYLAIRSDDQLENRFEPFTL
                     PRWKPNEGARSLLASFAAAFPLRRPSTIATEDMARYLLSRSEGTIGELTHLLTDAAVA
                     AIESGEEAINQRTLLLAPYVGPTERRRMFERELA"
     gene            10207..11490
                     /locus_tag="PLF56_01450"
     CDS             10207..11490
                     /locus_tag="PLF56_01450"
                     /inference="COORDINATES: protein motif:HMM:NF018259.2"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="TniQ family protein"
                     /protein_id="HOQ52260.1"
                     /translation="MTRRWPLHPPPGEGEALTSWLSRLAEVYGMSVEDLLRHSLAAPG
                     VDLADHHTSALDLDPPPWLLPALAERTGEPLGRLRQLTIAGWAPWLLDSLDPEPVPGA
                     AFDTYVRQDSVLLTARERPGRQVPGWRAWLPIDPKRGPLRRACPTCQRTGADGTFGFT
                     LVSQLPLTLSCPQHSCRLEPAFGGMGAFVAWEKPDTPARPAPAVVVAVDARTHQGLRT
                     GAILLPRRSVHVGVWFRLLRTLIDELSTPLSALRTGSRRAVRRIWEMADQAPRAGITG
                     PWRPYEALLWPQQQLLLEAAAATLALIETGDITAHGTLAPLLTPEPRRHVPDGTPPAP
                     RARDYWQEARDAMNMAIALAQKDPVAARQLLATLTGLTRSEATYQRIRDDLIALGVPN
                     DHLPPTLAEHASARPGNAGNECHISPAMARSEDLK"
BASE COUNT         1913 a         3796 c         3989 g         1877 t
ORIGIN      
        1 gactcggcca ccaagactcc ggccggactg ggctggctcg gcgcgctcgg cgtggccctg
       61 ctggtcgtgg gcatcggcgc gtccctcggt ggaccgacgg gctacgcgat caacccggcc
      121 cgtgacctcg gcccccgcat cgcccatgcg gtgctaccga tccccaacaa gggcagttcc
      181 gactggggct actcgtggat tccggtggtc ggcccgatca tcggcggtgt ggcggccggt
      241 ctgctgtcgc actccttgct gccgtgaggc acaatccgag ctagtacaca gtgctgggcg
      301 gcaacttctg gtgtcgccgc ccagcgcagg gcaccatgta tgaatcctct acaacgatgt
      361 ggaaaggcac acaatggctg agaagtacgt tctggcgatc gatcagggca ccacgagttc
      421 ccgcgcgatc gtgttcaatc acgcgggccg tatcgtcggc acaggccaga aggaacacga
      481 gcagatcttc ccgaaggcgg gttgggtcga gcacaacgcg atcgagatct gggacaacat
      541 tcgtgaggtg gtcggcatcg cgctggccga cgcctcggtg aacaaggaca acctggtggc
      601 agtcggcatc accaaccagc gcgaaaccgc gctggtctgg gacaagacca ccggcgagcc
      661 cgtctacaac gcgatcgtct ggcaggacac ccgcaccaac aagatcatcg agcaactcgg
      721 cggcggcgac caggacaagt acaaggaccg cgtcggcctg ccgctggcca cctacttctc
      781 cggccccaag gtcaagtgga tcctcgacaa cgtcgagggc gcccgcgaga aggccgaggc
      841 cggcgatctg gtcatgggca acatggacac ctgggtgatc tggaacctca ccggtggcac
      901 cgacggcggc gtgcacgtca ccgacgtgac caacgcctcg cgcaccatgc tgatggatct
      961 gaagtcgctg tcgtgggacg agagcatcgc ggccgacatg ggcatcccga tgtcgatgct
     1021 gcccgagatc aagtcgtcct ccgaggtgta cggccacggc cgcgaggccg gcctgctcgg
     1081 cggcgtcccg atcgccggtg acctgggcga tcagcaggcg gccaccttcg gccaggcggc
     1141 gttcgaggtc ggcatggcca agaacaccta cggcaccggc tgcttcatga tcatgaacac
     1201 cggcgaagag ctggtgccgt ccaagaacgg cctgctgacc accgtctgct acaagatcgg
     1261 cgacaacaag ccgatctacg cactcgaggg ctcgatcgcg gtcaccggtt cgctggtgca
     1321 gtggctgcgc gacaacctgc gcatgttcga ctcggctcct gaggtggagc agctggccac
     1381 tcaggtcgag gacaacggcg gcgtctacgt ggttccggcc ttctcgggcc tgttcgcccc
     1441 gtactggaag tcggacgcgc gcggcgcgat cgtcggcctg acccgctacg tcaaccgcaa
     1501 ccacatcgct cgcgcggtgc tggaggcgac cgccttccag acccgtgagg tgctggacgc
     1561 gatggaggcc gattcgggtg tgcacctggc cgagctcaag gtcgacggcg gcatgacgat
     1621 gaacgacacc ctgatgcagt tccaggccga tcaggtgggc gtgccggtgg tgcgtccggt
     1681 ggtggccgaa accaccgcgc tgggtgccgc ctacgccgcc ggcatcgcgg tgggcttctg
     1741 gaacggcgag caggacgtca tcgacaactg ggcagaggac aagcgctggg agccgtcgat
     1801 ggacgagggc gagcgcgagc gtctgtaccg gcagtggaag aaggcggtca cccgcacctt
     1861 cgactgggtt gacgacgacg tcaaggagtg acaccctccg ctgatgcggc gatcgcttga
     1921 acgaagcggg cccggcaccg ggcgcagttc cggtgccggg tccgcacgcg tgttggcgtt
     1981 ctgcctgttg ttggagttct gccggcgggg ggtgcacctc gaatggtgat cagccggcgc
     2041 gagcggtcgc tggacacgct gaccgccctg gcccaggctt tggcgggcga gttttcgcct
     2101 gcaaccgttg ctggagcgca ttctgcgcag cgcggtcgag ttgctgcaca gtgcgagcgg
     2161 ttcgctgtgc ctggtcgatg agcaggccca cacctaccac aaggaaatcg acctggacgc
     2221 gggctgcgag accgggcgtc cactgtcact cgacgagggc atgaccggcc tggtcgtgcg
     2281 tagccgtggc ccggtgattc ttgagcagta cgcgcaggtg ccgcggggcc acctcgctgc
     2341 gggtgaaccc cggtaccacc gggcggtcat cggcgcgccg atcatgctcg gcgaccaact
     2401 gatcggggcg ctggtggtgt tcgccggcga cggccagcgt tacggctccg acgacgcccg
     2461 gctgttgcag gagttcgccg gccacgccgc gatcgccatc gcgaactcga gggtgtacgc
     2521 gatggctgag gagcgtgcca aacaggtggc cgtggcggcc gagcgcgagc gttcgatgca
     2581 ggaggtgcac gacacgttcg gacgtggcct ggccaccgtc atgctgcggc tacaggcggc
     2641 gcaacgggcg gcggcgtccg ggtctggggt ggcggaccag ctcgccgccg cccaagcagc
     2701 cgtagacgac gccctgcgcg aagggcgtcg cgcggtctgg ggcactggct cgctcggcat
     2761 cggcgacact cgcacgctcg acgaagcgat tcgactcgaa ctcgattggg ccgccgcgac
     2821 cgccaacctg aacaccaact tccgggtgtt cggcgaccgg ggcgagatcg cgcccgaggt
     2881 gggcatgcag ctgctgcgca tcgtgcagga ggccctgacc aacgtggccc agcatgcgcg
     2941 ggcgtccgcg gttcggatcg gcctggtgtt cggcaccgat ggtgtggccg tgatcgtcga
     3001 ggacgacggg gtggggttcg acgtcgaggc ggtccggcaa cgccggaccg gcatgggcct
     3061 ggcgggcctg gtgtcccggg ccacgcaggt gggtggccgg gtccagctcg aatcgactcc
     3121 cggctggggt acccggatcc gcgccgactt gccctatcgg cccacggcgg gcgagcgcga
     3181 cggcgtctcg cgctggcggg tggtgatcgt gcacgaactg cccgccatgc gggccggctt
     3241 ggtgcagttg ctcgacgaat ccgagccggg ggtccaggtg gtcgccgagg tgtgtgactc
     3301 gcatcggtcg gtggcggtgt tctgcacgtt cggtggcggg ccactgcgtc ccttgctcga
     3361 ccacgacgca cgttgggtcg acaatccagc agagagagct cacccagaac ccactcttga
     3421 caggggattt taggaggcct aacgtcccac tcaagaaccc cctggagatg gaggcactat
     3481 gtcgcgaaat gttatacgca ccgccgttgt atctgcggcc agccttgcgc tggttgcact
     3541 gggcgccgct gcaccggcac gcgcagacgt cggcgagaac ggaaccaagt actgtggggg
     3601 gtcgaccccc ttctcatacg tgcagttcaa gacgaagggc taccgctcgt gggtagcgcc
     3661 gggtaagggt acgcaatacg gggaatcggg ctccacgagc agctggtaca ccggctcacg
     3721 gcagggcgtc gatggtggcg gctcttggtt cgccagcggt gccacgaacc ttgatcaggc
     3781 atataccaac ggggtttgca agaactacgg ctgagcaagc ttcagcagtg gcaatgcgag
     3841 ccgtgcggct gttgggcgtg gccgcttgcg tgattgtgac tgggtgcgtc acttcggcga
     3901 ctcctagtgg aagcccgagt acccccagcg tgttcgtggg caccaggacc gagtacatgc
     3961 aggctgtcgt ccagtgcttg gtgaaaaagg gcgtgagcgc acggttcgaa gtggccgagg
     4021 acggaacccc gagggtcgcg atgccggcga ccaccaaggc acagattgat aggaacaatg
     4081 cggcccagga ggagtgcagg aaggaactcc cgccaacgcc agaaccgacc acggatgcag
     4141 actttcgcga gttctatgcg catttgaatg ctgaggcagc atgcgtgcgc caggagggct
     4201 acgaaactgc gccggcgccg tccttccaaa agtttcttga gtcagcgcgc ggcaccggga
     4261 ttgattggga tccgttcagg gacgtgcctc cccaactcgt ggacagtgtg cgggcgaagt
     4321 gccagaccga tccgaacaag tggtggtagc cccccggttg gtcacgtggt cgttcggatg
     4381 aaatgggaac gttcacggaa cgcgtagttc ggtgagcggg tcacgacgtg ctgcgacgat
     4441 gctgggtggg atggctccgg ttcccgcggc ggcactggtg agtatcagtg ttgccgcgat
     4501 gaagtcccag ccagggaggg gttctcctcc ggcgacgagc gcagccatag caatggttcc
     4561 gccgagaagg ctggcggcga cagccacgta gagagtgctg atggtgacga gcgccacgat
     4621 gagtgaccgg gtcgcgccga gggcgcgtcg gcgcccaaag tccttgcgcc gaatcatcac
     4681 cagcccgaag agcaaggccc caacgagtgc tgctaagaca gtggtgaggg taagggtcag
     4741 gctcctgccg aagccgccga gctgaccttg gacgagggcg cgcagacggg cgatgtcggg
     4801 ggaggccgtg acgctgacgg cggatgcatc gaggggcgaa agaagggccg tggttgcggc
     4861 ggccactgcg gctacctgct cgggatggcg ggccagtacc acgacgatcg ccacttgtgg
     4921 ctcttgctcg gcggtcgacg gctggggtag cacaaccaac ggttcaaact gagccagaaa
     4981 tgccggagtg gtgagtcgcc cggtgacgtc gcagacaggg gtgccatcgc tggccaggta
     5041 gcctgcggcg tccggcatgc ctaacacggt caatgcttgg gtgcttgcgt agcacccctc
     5101 agcattggtt ggggaaatgt ggagaggggt caagtcggtg gcgtagagcg cccttaccgc
     5161 cacagacacg tcaccgccgg tgccgccgtt ggttgcgtct tgagcagcgc caaaggcgcc
     5221 cacccattcc gcgtcctcga tggtgttcag ccgcgccacg acggtggtgt cgagtccggc
     5281 cttgggttcg gctcgcacca ctacggcgcg ggagccggcg tcgtcgatgc tagcgaggat
     5341 ggcccgctcg gcggcggccg tgcgtccggc ggtcagcagc accgtggcgc ccatacctcc
     5401 gacgaggacg agggtgagta tcgacgcgac tcgttgtgac agtgccatcg cccatgcttc
     5461 agcgagcact ttgcgccacc gttcggttcg ggtcatcaca ggggcactga tcggtcgcag
     5521 gcgcgctcga ggcgagggtc gtgtgtcaca acgattactg ctcggccggt ttgggagtgg
     5581 cgacgcatag cgtcgatgac gccgtcggcg ctggcatcgt cgaggttgcc ggtgggttcg
     5641 tcggccagta gcaccatggg gtcgtgcacc agcgcgcggc acagggcaat gcgttgcgct
     5701 tgacctccgg aaatttgccc gggtctccgt tcggcgggca cttgaacacc cagctgttcg
     5761 atcagggcta ggccgcgttc gcgtgccgtt tgccggtcga ggtcgctgta gaggcagact
     5821 tcggtgatgt tgtcgatcac ggttcgggtc tggtcaagtg cggcgtcctg aaacacgaag
     5881 ccgagcagcc gggcacgcag cgcggaccgc tgggcgtcgg ggagtccgga aaccggctcg
     5941 cccttgacca gaactcggcc agcccggggg gacagcatca ggcccagcag gtagagaagg
     6001 gtcgacttgc cgcgtccgga gggaccggtg atggccacca gctcgcctgc ctcggcagac
     6061 agcgagacgc cggtcaggat gctgggtagg ttctcgtagc cgaattccag ctgctcgcag
     6121 ccgaacgcag tcattgccct ggcactcgca cagccgtacc cgccgctaga ccttcaacaa
     6181 ccgccatacc tcgggctcga gccacgactt taaccgtcac cctctgtccg tcagcggcga
     6241 tcacaccggg cgaaccattc gcgtcggaca caagtgctgc cagtgggacc accacaccgg
     6301 cgactgattg ctgcacttcg gtggtgccct tcagctgggt cgtgccctga agaggaacct
     6361 tgccgcactg ctccccgcac aaggtcctgc cttccgcagc cctcacaggt gccttcaccg
     6421 agccgtcctg ggtcacggta agcgggccca ccgatccttt ccatcgctgc gtgccgagat
     6481 cgactgtgat cgccatacct tcggtgacct gcttggcttg aaggtcgctg tactggacgg
     6541 tgaaccttgg cgcgctcgcc aaggcctcga tagcggggtc acccgctgac acctggccgc
     6601 cgaccgcaaa ctttgaactg agcgcgaccc gaaccggcag cgtcttgacg aacagcacgt
     6661 cgccccggtc taggcgtccg gttcccgcta caccaagaga cttctgccac gaacgcaccg
     6721 cccgctgaac cgaagccgtg aaggtcgatg tcgtcgaggc aaggaacttc ttcgccacca
     6781 gaaaccgcgt caactgagtg acgtctgcgc cggacacccc aagggttaag gcgcgaaagg
     6841 cgggtacggc accgggtagc gccacgacgg ccctctcatc aacccgatac aacacgtcgc
     6901 cggcttcgac atcttcgccg cgcgacacca aaaggcgcgt gatcgtccct gatgcctggt
     6961 taaccgccac ctggcgaagt ggccatgacg cagtcaccac tgcggcaacc gactgaccta
     7021 tcgcaccctc cttcgcctcc acaagcacat aggttgactc ggccgccacc tgctgtggag
     7081 gtcgcgtagc caccgcaacg gcccaaccga tcacgccgcc tacgagcagg accgccaccg
     7141 cccatgcaag agcgaagcgc actctcacca ctgcccgacc caattctctt gcttgcctag
     7201 ccattcgtgg ggggctcctt tgcggcggga ttgcagggca ccgattggtc tgtcggaaac
     7261 cgatcgttgc gtacccaatc catgcttgga atgtagccgc cgttcgcggc cgccagccga
     7321 atcgccccgc caatcttagg aacttcaccg aatcacccat acggctcggg tgtcgttgac
     7381 cagtgtccgc cgacccttgt ctcagcaatc taaagattga tgagacagtc cgggtgaggt
     7441 gctgccagag acgcccattg tgaatgtccg aagtcggctg cgcaggtgac ctgcacgttc
     7501 gaggaagtgt gcaggggagt tcgatcgctc ggcgtgtccg tacccgtgtg cacgatccgc
     7561 tgttgttcca ttgtcgtgtg cacgaagaag caggccagga tgaccgggtt cctgagcgcg
     7621 gtctgttgac cgtccccgat gaggtgtggg cactggcggt gcgccgggcg aaggtgatcg
     7681 gcccgctggc cgcggcgggg acggccggtg aggaggcggt ggcggcggca gccgggcagc
     7741 tgggtgtctc gcgccgccag gtttacgtct tgctgcgccg gtggcgcgaa ggccagggcg
     7801 tggtctccga tctgatcccg gggcgctcca gcggtggccg cggcgggcag cggctggcgg
     7861 tcgaggttga ggaggtcatc cgggaggtgc tgcgcaagca ctacctgacc cggcagcgga
     7921 agacggtggc tgcggtccac cgcgaggtca ggcgggtttg ccgtgtccgc gggctgccgg
     7981 tgccgtcacg cggcgcgatt gtgcgtcgca tcgccggcct tgacccgcac gcggagaaga
     8041 cagcccgtga gggagcagac gccgctcggg cgttggagtc cgccgggggg caggtgccac
     8101 cggtgatcgg ggtgctggag caggtgcaga tcgaccacac cgtggtggac ctgatcgtgg
     8161 tcgacgaacg ccaccggttg ccgatcggcc gaccttacgt gacggtcggg atcgacgtct
     8221 attcccggtg catcgtgggc ctggtgacca ccttggaggc accctcggcg ctgtcggtgg
     8281 ggttgtgcct ggcgcacatg gtcaccgata agcgggcctg gctggaacgc ctgggggtcg
     8341 aggcggcgtg gccgatggct ggcaagcccg gcgagttgta tctggataac gccgcggagt
     8401 tcaagagcga ggccctgcgg cggggttgtg aggagcacgg gatcacgctg cggtaccggc
     8461 cgccggggcg cccgcacttc ggcgggatcg tcgagcgggt catcggcacg ctgatggaga
     8521 tggtccacga gctgcccggc acgacgttct ccaacccagc ccagcgcggc agctacgact
     8581 ccgaagcgag ggccaccttg acagtggcgg agctggagaa gtggctggcc gtggcggtgg
     8641 cgagctacca cgggcaggtc cacggcacga cccggcagac cccgcaggct cgttgggccg
     8701 ccggcatcga gcaaaggtca ccgacgatgg tgggcgacga gacggcgttc ttggtggact
     8761 tcctgccggt gatccgccgg acgctgactc gcaccggatt cgtcatcgac cacgtgcact
     8821 atttcagcga cgcgctcaag ccgtggatcg cccgccgcgg ccggctggac cggttcgtga
     8881 tccgccggga cccgcgcgac atcagccgca tttgggtgct cgatcccgat ggtgggtcct
     8941 atctgacggt gccttaccgg acgctgtcac atccgccggt gagcgtgtgg gagcaccgca
     9001 ccgcggtgga acgactccgg gaggagggtc gggcgcaggt cgatgaggag ggactgttcc
     9061 ggatggtcga gcagatgcgc actctcatcg acaccgccgc cgcgaccacg cgcaaggccc
     9121 gccgcgacgc tgaacgccgc agcaaagccg gtaccgcagg tccacgggac aagcccgcgt
     9181 tggcggcccc gcccgacgac accttggctg atggcaccag cagtagccgc gccgctgatg
     9241 cggggtccgt gccgccgttc gaggtgatcg agcagtggtg aacgggggcg gctcctgggt
     9301 cggcgaggac caccagggcg atgccctgga tcatctgcat gtgagcgctc aggcggtggc
     9361 acggctgccg gcggaggagc ggttgcggca cgtgcgcgcc gaccggtgga tcggctactc
     9421 ccgcgcgact gcggcgctgg agcggctgga cacgttgtac gcgtggccgg gtaagcagcg
     9481 catgcctaat ctgctgttga tcggcccgac gaacaacggc aagtcgatga tcatcgagaa
     9541 gttccgccgg ctccacccgc ccacctctca cgccgatcgc gaacagatgc cggtgctggc
     9601 ggtgcagatg ccctccgatc cctcggtgat ccggttctac gtcgcgctgc tggccgcgct
     9661 gggagcaccg ctgcgccccc gccagcgcct ggccgagctc gagcaggcca ccttgacgct
     9721 gctgcgcgcg gtcggggtgc gggtcctggt catcgacgag ctgcacaacc tgctggccgg
     9781 gcgcggggag acccggcgcg agttcctcaa cctgatcagg tttctgggca acgagctgcg
     9841 catcccgctg gtcggggtcg gcacccggga ggcttacctg gcgatccgct ccgatgacca
     9901 gctggagaac cgcttcgagc ccttcacgct gccgcggtgg aaaccgaacg aaggtgcccg
     9961 gtcgctgctg gccagcttcg ccgccgcgtt cccgctgcgc cgaccctcga ccatcgccac
    10021 cgaagacatg gcccgctacc tgctctcccg cagcgagggc accatcggcg agctcaccca
    10081 cctgctcacc gacgccgcgg tggccgcgat cgagtccggt gaggaggcga tcaaccaacg
    10141 aaccttgctc ctggcgccct acgtcggccc caccgaacga cgccgaatgt tcgaacgcga
    10201 gctggcatga cccgtcgctg gcccctgcac ccgccacccg gtgagggtga ggcgctgacg
    10261 tcgtggctca gccgactggc cgaggtgtac ggcatgagcg tcgaggacct gctccggcac
    10321 agcctcgccg caccgggcgt ggaccttgcc gaccaccaca ccagcgccct cgatctggac
    10381 ccgccaccgt ggctgcttcc cgcgttggct gagcgcaccg gcgaaccgct gggccggctg
    10441 cgccagctga cgatcgccgg ctgggcgccg tggctcctgg actcccttga ccccgagccc
    10501 gttcccgggg cagcgttcga cacctacgtc cggcaggact cggtgctcct caccgccagg
    10561 gaacggcccg ggcggcaggt gcccggctgg cgggcatggt tgccgatcga ccccaagcgc
    10621 gggccgctgc gccgggcctg cccgacctgc cagcgcaccg gcgcggacgg caccttcggt
    10681 ttcacgctcg tgtcccagct cccgctgacc ctcagctgcc cgcagcacag ctgccgcctc
    10741 gaacccgcct tcggcggcat gggcgctttc gtcgcgtggg agaagccgga cacccccgcc
    10801 cgccccgcac cggctgtcgt cgtcgccgtg gacgcccgca cccaccaggg gctgcgcacc
    10861 ggcgccatcc tcctgccgcg tcgatccgtg cacgtagggg tgtggttccg gctgctgcgc
    10921 accctcatcg acgagctgtc caccccactc tcggcgctgc gtaccggctc ccgacgcgcc
    10981 gtgaggcgca tttgggagat ggccgatcag gccccacgcg ccggcatcac aggtccctgg
    11041 cgcccctacg aggcactgct gtggccccag cagcagttgc tcctcgaagc tgccgccgcc
    11101 acccttgccc tcatcgagac cggcgatatc accgcccacg gcaccctcgc cccgctgttg
    11161 acgcctgagc cccgccggca cgtccccgac ggcaccccgc ctgctcctcg cgctcgtgac
    11221 tactggcagg aggccaggga cgcaatgaat atggcgatcg ccctcgccca gaaggaccct
    11281 gtcgccgccc ggcagctgct ggccaccctc accggcctga ctcgcagcga ggcgacctac
    11341 caacgcattc gcgacgacct gatcgccctc ggcgtaccga acgaccacct cccaccgacg
    11401 ctggccgagc acgcatccgc tagacccggc aacgccggaa acgaatgtca catctcccca
    11461 gcgatggccc gttcagagga cttaaaatag gtgacaggtt aagtgacagt acgcatggcg
    11521 tgtggcttcg ttcgcttggt ggcggggttt ttgcagcgct ccgtggcggt cttgt
//
LOCUS       DAOSUG010000022        11148 bp    DNA     linear   ENV 10-JUN-2024
DEFINITION  MAG TPA_asm: Micropruina sp. isolate AMR_MDS_2195
            NODE_3297_length_11148_cov_4.224015, whole genome shotgun sequence.
ACCESSION   DAOSUG010000022 DAOSUG010000000
VERSION     DAOSUG010000022.1
DBLINK      BioProject: PRJNA925477
            BioSample: SAMN32864262
            Sequence Read Archive: SRR4236673
KEYWORDS    WGS; ENV; Metagenome Assembled Genome; MAG; Third Party Data; TPA;
            TPA:assembly.
SOURCE      Micropruina sp. (wastewater metagenome)
  ORGANISM  Micropruina sp.
            Bacteria; Bacillati; Actinomycetota; Actinomycetes;
            Propionibacteriales; Nocardioidaceae; Micropruina.
REFERENCE   1  (bases 1 to 11148)
  AUTHORS   Abdulkadir,N., Saraiva,J.P., Zhang,J., Stolte,S., Gillor,O.,
            Harms,H. and Rocha,U.
  TITLE     Genome-centric analyses of 165 metagenomes show that mobile genetic
            elements are crucial for the transmission of antimicrobial
            resistance genes to pathogens in activated sludge and wastewater
  JOURNAL   Microbiol Spectr 12 (3), e0291823 (2024)
   PUBMED   38289113
REFERENCE   2  (bases 1 to 11148)
  AUTHORS   Abdulkadir,N.
  TITLE     Direct Submission
  JOURNAL   Submitted (24-MAR-2023) Environmental Microbiology, Helmholtz
            Center for Environmental Research -UFZ, Permoserstr 15, Leipzig
            04318, Germany
COMMENT     The annotation was added by the NCBI Prokaryotic Genome Annotation
            Pipeline (PGAP). Information about PGAP can be found here:
            https://www.ncbi.nlm.nih.gov/genome/annotation_prok/
            
            ##Genome-Assembly-Data-START##
            Assembly Method        :: SPAdes v. 3.11.1
            Genome Representation  :: Full
            Expected Final Version :: Yes
            Genome Coverage        :: Not Applicable
            Sequencing Technology  :: Illumina MiSeq
            ##Genome-Assembly-Data-END##
            
            ##Genome-Annotation-Data-START##
            Annotation Provider               :: NCBI
            Annotation Date                   :: 05/04/2023 04:57:10
            Annotation Pipeline               :: NCBI Prokaryotic Genome
                                                 Annotation Pipeline (PGAP)
            Annotation Method                 :: Best-placed reference protein
                                                 set; GeneMarkS-2+
            Annotation Software revision      :: 6.5
            Features Annotated                :: Gene; CDS; rRNA; tRNA; ncRNA
            Genes (total)                     :: 2,776
            CDSs (total)                      :: 2,750
            Genes (coding)                    :: 2,714
            CDSs (with protein)               :: 2,714
            Genes (RNA)                       :: 26
            tRNAs                             :: 24
            ncRNAs                            :: 2
            Pseudo Genes (total)              :: 36
            CDSs (without protein)            :: 36
            Pseudo Genes (ambiguous residues) :: 0 of 36
            Pseudo Genes (frameshifted)       :: 2 of 36
            Pseudo Genes (incomplete)         :: 32 of 36
            Pseudo Genes (internal stop)      :: 2 of 36
            ##Genome-Annotation-Data-END##
FEATURES             Location/Qualifiers
     source          1..11148
                     /organism="Micropruina sp."
                     /mol_type="genomic DNA"
                     /submitter_seqid="NODE_3297_length_11148_cov_4.224015"
                     /isolate="AMR_MDS_2195"
                     /isolation_source="Activated sludge of municipal
                     wastewater treatment plants"
                     /db_xref="taxon:2737536"
                     /environmental_sample
                     /geo_loc_name="Canada"
                     /lat_lon="49.25 N 123.1 W"
                     /collection_date="2015-06-16"
                     /metagenome_source="wastewater metagenome"
                     /note="metagenomic"
     gene            <1..1018
                     /gene="tsaD"
                     /locus_tag="PLF56_01455"
     CDS             <1..1018
                     /gene="tsaD"
                     /locus_tag="PLF56_01455"
                     /EC_number="2.3.1.234"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_002525914.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_process: GO:0006400 - tRNA modification [Evidence IEA]"
                     /codon_start=2
                     /transl_table=11
                     /product="tRNA
                     (adenosine(37)-N6)-threonylcarbamoyltransferase complex
                     transferase subunit TsaD"
                     /protein_id="HOQ52261.1"
                     /translation="ESSCDETGVGIVRGHELLANEVASSVEQHVRYGGVVPEIASRAH
                     LEAMRPALERALATADVDLSELDGIAVTAGPGLMGALVVGLASAKALAASLDKPLYGL
                     NHLVGHVAVDLLDHGPLPQPCAALLVSGGHTSLLLVNDITGDITELGSTIDDAAGEAY
                     DKVARLLGLAYPGGPVIDRAAADGNPRAIAFPRGLTAPKDLVKHRFDYSFSGLKTAVS
                     RWVETRRRDGDEVPVDDVAASFQEAVCDVLTAKAVDACEHLGVSDLLIGGGVAANSRL
                     RALLEQRTSERGITLRRPRPALCTDNGAMIAVLGAEVIGRGLRPSSLDIGADSGLPVS
                     TVLV"
     gene            1022..1516
                     /locus_tag="PLF56_01460"
     CDS             1022..1516
                     /locus_tag="PLF56_01460"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_002549919.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="8-oxo-dGTP diphosphatase"
                     /protein_id="HOQ52262.1"
                     /translation="MPYTPILGVLAYVLHPDGERVLLVLRSRDGDAHRGKYNGLGGKV
                     EPDESVIGCLHRELMEEAGITVTGQRLRGTISWPGFGAGGEDWFGFVFIVDGFTGDVP
                     ERNDDGPLEWTSIARLLGAGAPLPMWEGDRHWLPLVFDDDERAFHGVMPYVDGRPASW
                     SFDR"
     gene            complement(2190..3080)
                     /gene="mmuM"
                     /locus_tag="PLF56_01465"
     CDS             complement(2190..3080)
                     /gene="mmuM"
                     /locus_tag="PLF56_01465"
                     /EC_number="2.1.1.10"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_013865992.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_function: GO:0047150 - betaine-homocysteine
                     S-methyltransferase activity [Evidence IEA];
                     GO_process: GO:0009086 - methionine biosynthetic process
                     [Evidence IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="homocysteine S-methyltransferase"
                     /protein_id="HOQ52263.1"
                     /translation="MDSFASALREGVVLLDGGFATQLEARGHDLSDSLWSARLLLDEP
                     DEIRATHADFFAAGAQVAITASYQVSETGFVAAGRSAAEAREALTRSVRLAREAASGC
                     DGQRWVAASVGPYGASLADGSEYRGDYGLSVAKLRQWHRPRLTALAEAEPDLLACETI
                     PCLAEVEALVTELSELDLPAWISLTVVGDRTRLGEPADEAYAMAAEVPQVVALGANCC
                     DPGGAADVVKAVAGRRPVVFYPNSGEAWNARSRTWTGAAAASTSVRAWLDAGVRAVGG
                     CCRVTPDQIAAMRLQLDERG"
     gene            complement(3106..3435)
                     /locus_tag="PLF56_01470"
     CDS             complement(3106..3435)
                     /locus_tag="PLF56_01470"
                     /inference="COORDINATES: ab initio
                     prediction:GeneMarkS-2+"
                     /note="Derived by automated computational analysis using
                     gene prediction method: GeneMarkS-2+."
                     /codon_start=1
                     /transl_table=11
                     /product="hypothetical protein"
                     /protein_id="HOQ52264.1"
                     /translation="MKMPARILLIFGAVALVAAIGVLSYGGFEVWRQFLALDALRSRE
                     FANPVGVMIGGAALALLAGLLGGYALALPRHPKAPGAKGTKPIPAQQAEPTATDPSTN
                     PHPESLR"
     gene            3973..5127
                     /locus_tag="PLF56_01475"
     CDS             3973..5127
                     /locus_tag="PLF56_01475"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_015071169.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="SAM-dependent methyltransferase"
                     /protein_id="HOQ52265.1"
                     /translation="MDAEIARWLVSPEAADALARAGREADPGSLAAAQRLRRDLPPDH
                     AAAVVNQVVLRRRAVAKLGAQAAQLYLTSDGLEQATRPAVAAWRAARFAAAGVRTVLD
                     LGCGIGADALAFAAAGLEVIAVESDEATAVLASANLGREVLVGDAVTMLDDLDPDAAV
                     FCDPARRTSAGRSWRVDDLSPPWDFVAELLQRRTACIKLGPGAPHALIPRDVAATWVS
                     ERGDLVELSLWSAGWPPAREAVVLPAGAVLGGAAAFSGGEPATPRVGDVLYEPDPAVI
                     RAGLVDRLAGDLDATRLHPGIAYLLGPRPQPTPFATAFTVLDVMDAGERTLRAWVRRE
                     RIGTLEIKKRGIDIDPAALRRRLKPAGPASATVVLTPTVDGARALVAQRL"
     gene            complement(5421..6116)
                     /locus_tag="PLF56_01480"
     CDS             complement(5421..6116)
                     /locus_tag="PLF56_01480"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_020577114.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_function: GO:0003824 - catalytic activity [Evidence
                     IEA];
                     GO_function: GO:0043546 - molybdopterin cofactor binding
                     [Evidence IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="MOSC domain-containing protein"
                     /protein_id="HOQ52266.1"
                     /translation="MSASVLSVNLGRPAVDRGKRSNTTGIDKQPVPSAAVSAPGARAV
                     TRRLGPEHRSGLAGDYIGDGKHHGGDVQAVYAYAREDLDLLGAQVGRQFASGSFGENL
                     TTLDLDVTGALIGEHWRVGTGPDAVELAVTCPRIPCNTFRAWIAERGWLKLFTKAAVP
                     GAYLSVLAPGVVRPGDPIEVTFRPEHEVTIGMLFRSQTLERELAPLVLTAADYLDAKS
                     LDYAQRGETFSID"
     gene            complement(6113..7426)
                     /locus_tag="PLF56_01485"
     CDS             complement(6113..7426)
                     /locus_tag="PLF56_01485"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_015583109.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_function: GO:0003824 - catalytic activity [Evidence
                     IEA];
                     GO_process: GO:0005975 - carbohydrate metabolic process
                     [Evidence IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="alpha-amylase family glycosyl hydrolase"
                     /protein_id="HOQ52267.1"
                     /translation="MDWTGHVIWWHCYPLGFVNAEDTAVHEVRHRLGQLTNWLDYLLD
                     LGCNGMLLNPIFASSTHGYDTLDHYAIDSRLGDDGDFGELMWKAKERGIRILLDGVFN
                     HLGRAHPIVQRALTAGPGTPEGDWLRWVEGYPRVFEGHEQLVELDFTNPAVQDFVVEV
                     MSHWLDRGIDGWRLDAAYAPGAAAWRPIVERVKAAHPDCWLLGEVIHGDKDEFVELSG
                     LDSVTQYELWHGIWQSMNDHNLWSLDWSLQRHRRFCEHFRPQIFISNHDVTRISSKLD
                     DRRHIQHAAVLLTLLPGVPSIYAGDEHRFEGIKLDQIGGDDAVRPPFPQTPDGLLPFG
                     ADTFDLYRSLIGLRRRFPWIVDGIATTRDLTDESMVVLVTGPDQVIELALNISDEVGP
                     MPIGEVLLASPGCAETVPPHGWAVGVRPRDEEDVAFVPREVRGAR"
     gene            7624..8418
                     /locus_tag="PLF56_01490"
     CDS             7624..8418
                     /locus_tag="PLF56_01490"
                     /inference="COORDINATES: protein motif:HMM:NF013961.2"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="transglutaminase family protein"
                     /protein_id="HOQ52268.1"
                     /translation="MQRFVSAHIEATLDVPTDVVLAIAVAEGPERSEQLTMTANGAPA
                     FAREIAAPHGGRLHLVEGLPPGPLTIDYGASLDGLAEPEVPTDLDRITYARPSRYCDS
                     DKLMPVAQAEFAGLEGKLLLDAVSSWVGSQLSYVSGSSKPIDGAVDTYLLRQGVCRDY
                     AHLVIALLRGLNVPARLAAVYAPGLDPMDFHAVAEAYVDGAWWVVDATTLAPRPSLVR
                     IATGRDAADTAFLSTLKGSLTLHSVLVGATVDALPGDDLTLLTALR"
     gene            8681..10891
                     /gene="treS"
                     /locus_tag="PLF56_01495"
     CDS             8681..10891
                     /gene="treS"
                     /locus_tag="PLF56_01495"
                     /EC_number="5.4.99.16"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_013861358.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_function: GO:0047471 - maltose
                     alpha-D-glucosyltransferase activity [Evidence IEA];
                     GO_process: GO:0005991 - trehalose metabolic process
                     [Evidence IEA];
                     GO_process: GO:0009269 - response to desiccation [Evidence
                     IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="maltose alpha-D-glucosyltransferase"
                     /protein_id="HOQ52269.1"
                     /translation="MTVEPDDQASTEIDDVLPEMTFDAQFYPARPKKLRPAARRRNPL
                     HAISAPPFAPDGRNKAYVEWLVQQSMLGDARTLARQLAGLHTMWSNPFAAPDPRAAVD
                     RASVWFTAYPLSLVTAPNQSFLGALGDDDLWSCFESVGVDAVHTGPVKLAGGIRGWDA
                     TPSVDGHFDRISMAIDPLFGTEEEYRGMCETAGRHHAMIIDDIVPGHTGKGADFRLAE
                     LNYGDYPGIYHLVDIPPEAWHLLPEVPKGFDAVNLDPSHERALAEAGYIIGELQRVIF
                     YEPGVKETNWSATPPIYDHTGTLRRWVYLHYFKEGQPSINWLDPTFAGMRLVIGDALH
                     SLLDLGSGGLRLDANGFLGVEKNVSGPAWSEGHPLSQAANQLIGSMVRKVGGFTFQEL
                     NLGIDDIAETSKVGPDLSYDFVTRPAYQFALATGDTEFLRLTLREAMAAGIDQASLVH
                     ALQNHDELTYELVHFAGRHAHDTYTLGGVTYSGTDLAEHVRETLRLKITGDAGPYNAV
                     FTQNGIASTTASVIAASLGYDDLDAIGPSEVERIKSAHLLLSMYNAWQPGVFALSGWD
                     LVGALPLKRSQVESLIATGDTRWIERGAYDLMGWSPEATTSSSGMPLARALYGSLPEQ
                     LADSKSFVSRLSAILKVRKRHGFFSGSLLDVPEVPTKEMLVLVNRLFVGAIQITALNF
                     GPEKIQGHVQSAQIPPGRVFDLSTRRKIDNVDDLGGFNVTLPPFGGLAMLVREG"
BASE COUNT         1686 a         3909 c         3814 g         1739 t
ORIGIN      
        1 cgaatcgtcg tgcgacgaga ccggggtggg catcgtccgc gggcacgagt tgctggccaa
       61 cgaggtcgcc agttcggtcg agcagcacgt gcgctacggc ggcgtggtgc ccgagatcgc
      121 gtcgcgggcc cacctggagg cgatgcggcc ggcgctggaa cgggcgttgg ccaccgctga
      181 tgtcgacctg agcgaactgg atggcatcgc cgtcaccgcc ggcccaggcc tgatgggggc
      241 cctggtggtc gggttggcat cggcgaaagc cctggccgca agcctagaca agccgttgta
      301 cgggctgaac cacctggtcg gtcacgtggc cgtcgacctg ctcgaccacg gcccgctgcc
      361 gcagccgtgc gcggcgctgc tggtctcggg cgggcacacc tcgctgctgc tggtgaacga
      421 catcaccggt gacatcaccg agctcggatc caccatcgac gacgccgccg gcgaggccta
      481 cgacaaggtc gcccggctgc tcggcctggc ctacccgggc ggtccggtga tcgatcgggc
      541 ggccgccgac ggaaacccgc gggcgatcgc ctttccgcgc gggctgaccg caccgaaaga
      601 cctggtcaag caccggttcg actactcgtt ctcgggtctg aagacggcgg tctcgcgctg
      661 ggtcgagacc cgccggcgcg acggtgacga ggtgccggtt gacgacgtgg cggcctcgtt
      721 ccaggaggcg gtgtgcgacg tgctgaccgc caaggcggtc gacgcctgcg aacacctcgg
      781 ggtctccgac ctgctgatcg gcggcggcgt ggcggccaac tcgcggctgc gggcgctgct
      841 ggagcaacgg acgtccgaac gcggcatcac gctgagacgg ccacggccgg cgctgtgcac
      901 cgacaacggg gcgatgatcg cggtgctggg ggccgaggtg atcggtcgcg ggctgcgtcc
      961 ctcgagcctc gacatcgggg ccgactcggg cctgccggtc tcgaccgtgc tggtctgaag
     1021 catgccgtac acaccgattc tcggggtgct ggcctacgtg ctgcatcccg atggtgagcg
     1081 cgtgctgctg gtgctgcgca gccgcgacgg cgatgcccat cggggcaagt acaacgggct
     1141 cggcggcaag gtcgaacccg acgagagcgt gatcggctgc ctgcaccgcg agctgatgga
     1201 ggaggccggg atcacggtca ccggacagcg cctgcggggc acgatctcgt ggcccggctt
     1261 cggggcgggc ggcgaggact ggttcggttt cgtcttcatc gtggacgggt tcaccggcga
     1321 cgtgcccgag cgcaacgacg acggaccgct ggagtggacg tcgatcgcgc gcctgctcgg
     1381 cgccggggct ccgctaccga tgtgggaggg cgaccggcat tggcttccct tggtgttcga
     1441 cgacgacgag cgggcctttc acggggtgat gccctacgtc gacggacgtc cggcctcgtg
     1501 gagctttgac cgctgaccgc tgaccgctga ccgctgaccg ctgaccgccg agcgagcgtc
     1561 gtgcgtcggt cgagcttgtc gacacatgcg ttggtcgaga tgtctctggt gggtcggtgg
     1621 cggagggggg ctctactcga gccaaccagc ctcggtcgca ggctccctcg gcggccagac
     1681 ccatccacga ctcgagtaga gccccccctc cgccccctcc actctcgctg tggaccactc
     1741 acgtcgggcg gcctcgctcc ggacctccgc tggtcgagac ctccgccccc tccactctcg
     1801 ctgctgacca ctcacgtcgg gcgcactggc tccggacctc cgctggtcga gcgtgtcgag
     1861 acccatgctc tgggccaaac atctggggac ccgcgctcgt ccgcgatctc gacatcggcg
     1921 ttggatggcg ggataccttg ccggtttcga gtgacaaccc gaaaccggac gggtcttgct
     1981 cttgtcgggt agcgatctcg acaccggcac tgatctcagt gtcgggttcc tgatcctgct
     2041 cacggtctgt tatcggccat cgatctcgac acctggattg gcacgtctcg gcgatcttcg
     2101 gcaaaccccc gacgtctcgg gggcgaatgg ggggtctcga cgctcaaccg tcggtctgcc
     2161 ggactcaccc catcgctggc gccgcgagct cagccccgct cgtccagctg aagccgcatg
     2221 gcggcgatct ggtcgggggt gacccggcag cagccgccga cggcccgcac gccggcgtcc
     2281 agccaggctc gcacgctcgt ggacgctgcg gccgccccgg tccaggtgcg cgagcgggcg
     2341 ttccaggctt caccgctgtt ggggtagaac accaccggac gccgccccgc caccgccttc
     2401 acgacgtccg cggcgccgcc gggatcgcag cagttggctc ccagcgcgac cacctgcggc
     2461 acctcggcgg ccatcgcgta ggcctcgtcc gcgggctcac ccagccgggt gcgatcgccc
     2521 accacggtca gcgagatcca ggccggcagg tcgagctcgc tcagttcggt gaccagtgcc
     2581 tcgacctcgg ccaggcaggg gatcgtctcg cacgccaaca ggtcaggttc ggcctcggcc
     2641 agcgccgtca atcgcggacg gtgccactgc cgcagcttcg ccacgctcaa cccgtagtca
     2701 ccccggtatt ccgagccgtc agccagactc gcgccgtagg ggcccaccga ggcggccacc
     2761 cagcgctgtc cgtcgcaccc ggacgccgcc tcgcgagcaa gccgcacgct tcgggtcagt
     2821 gcctcgcgcg cctcggcggc ggaacggccc gcggccacaa agccggtctc gctgacctgg
     2881 taagaggcgg tgatcgccac ctgggcaccc gcggcgaaga agtcggcatg ggtggcgcgg
     2941 atctcgtccg gctcgtcgag cagcaggcgc gccgaccaca gcgagtcgct cagatcgtgg
     3001 ccgcgcgcct ccaactgggt ggcgaaacca ccgtcgagca gcacgacgcc ttcgcgaagc
     3061 gcagaagcga acgaatccat gcccgtcatc gacctgtcgg cacgctcagc gcagcgactc
     3121 gggatgcggg ttcgtcgacg ggtccgtcgc tgtcggctcg gcctgctgag ccgggatggg
     3181 cttcgtgccc ttggcgcccg gtgccttcgg atggcgcggc aatgcgaggg cgtagccgcc
     3241 gagcagcccc gccagcaacg ccaacgccgc cccaccgatc atgacgccga ccgggttggc
     3301 aaactcacgg ctgcgcaggg cgtcgagcgc gagaaactgg cgccacacct cgaacccgcc
     3361 gtaggacagc acaccgatgg cggcgaccag agccacagct ccgaagatca gcaggatccg
     3421 cgcgggcatc ttcatggcgt catcctaatc ccggatccat cgatcgccgc gtgcgctttc
     3481 cgggcacccc tggctgcgcc ggccacgacg tgcacacacg ggcgctcgcc gccgacaaac
     3541 accaaggacg tcggctgcgc tcgcaagtga cagatgccgg tatgcctcgc cgcggagcga
     3601 cctgcgcccc ccgtctgacg ttgctcgcga tggcggccat cggtgggtcc gagccaggac
     3661 gcgccagtgc aagattcgtt ggttgggctc gtggaaaccg ctgcccggag gtctcgacca
     3721 gcgattgatc ggcggtttcg atagtgggtg ggtttcggca ccgactgcgg tatcaaaacc
     3781 cacccgctct cgctcggtgt gccgacgccc gctcgcgact gctgcccacg gacccgcttg
     3841 gccatccttc tgccgccact cgcaacggcg aagcgtcggg ggatccggac tacgcaaccc
     3901 gactcgcggg ccgtggtggc gcgccgggcg tgaagagtcg acggctgcgt ccagatcggg
     3961 tagacaccag gtgtggacgc cgagatcgcc agatggctgg tcagccccga ggctgcggac
     4021 gccctcgccc gggccgggcg tgaagccgat cccgggtcgc tggccgccgc gcaacggctg
     4081 cgccgtgacc tgccgccgga ccacgcggcc gccgtggtga accaagtggt actgcgccgg
     4141 cgcgcggtcg cgaagctcgg tgcgcaggct gcgcagctct atctgacctc cgacgggctc
     4201 gagcaggcca cccgtccggc ggtggccgcc tggcgtgccg cacggttcgc cgcggcgggg
     4261 gtacgcacgg tgctcgatct ggggtgcggc atcggagcgg acgccctggc cttcgcggca
     4321 gcaggtctcg aggtgatcgc ggtcgagtcc gacgaggcga ccgccgtgct ggcctccgcc
     4381 aatctgggcc gcgaggtgct ggttggtgat gccgtgacga tgctcgacga cctcgatccg
     4441 gacgccgccg tgttctgcga cccggcgcgc cgcacgtccg ccggccgaag ctggcgggtg
     4501 gacgacctga gcccgccctg ggatttcgtc gccgagctgc tgcagcgacg caccgcctgt
     4561 atcaagctgg gtccaggggc gccccacgcc ctgattccgc gcgacgtcgc ggccacgtgg
     4621 gtgagcgagc gcggcgacct ggtggaactg tcgctgtggt cggcgggctg gccgcctgcg
     4681 cgcgaggccg tggtgttgcc cgcaggtgcg gtgttgggcg gtgccgcggc cttcagcggc
     4741 ggcgagccgg cgactccccg cgtgggcgac gtgctttacg agccggatcc cgccgtgatc
     4801 cgggccggcc tggtggatcg tctcgcgggt gatctcgacg ccacccgcct gcaccccggt
     4861 atcgcctacc tgctcggccc acgcccgcaa ccgaccccgt tcgccacggc cttcacggtg
     4921 ctggacgtaa tggacgcggg ggagcgcacc ctgcgcgcct gggtgcgtcg cgagcgcatc
     4981 ggcacccttg agatcaagaa acgcggcatc gacatcgacc cggcagccct gcggcgccgg
     5041 ttgaagcccg ccggccccgc gtccgccacc gtggtgctca cccccaccgt ggacggagcc
     5101 cgtgccctgg tggcacagcg cctctgaggg cctgagggcc tctgactcca aggccctgtg
     5161 ggttggggcc gcgcgatctc gacatcgccg cgcagtggcg ggaaaagctc gccggtttcg
     5221 tggtgcaacc cgaaacctga cgggtcgtcc tgacatcggg cagcgatctc gacatcgcac
     5281 tccaccggcg aaccgatcgc accggcaacg ggccaacgcc gaggggcccc actgaggcac
     5341 gcgtgtcaca tcctgaccag gcatgctcga ccagcgggcg cccggtgagc cggagacggt
     5401 aaccgttccc gctccatgca tcagtcgatg ctgaacgttt ctccacgttg cgcgtagtcg
     5461 agcgacttgg cgtccaggta gtcggcggcg gtcagcacca gcggggccaa ctcgcgctcg
     5521 agggtctgcg agcggaacag catcccgatc gtcacctcgt gttcagggcg gaacgtgacc
     5581 tcgatcgggt cgcccggccg caccactccc ggggcgagca ccgaaaggta ggcccccggc
     5641 acggcggcct tggtgaacag cttcagccag ccgcgctcgg cgatccaggc ccggaacgtg
     5701 ttgcacggaa tgcgcgggca ggtcaccgcc agttcgaccg cgtccggccc cgtaccgacg
     5761 cgccagtgct cgccgatcag ggcaccggtc acgtcgaggt cgagcgtggt caggttctcg
     5821 ccgaacgacc cggacgcgaa ctgccgcccc acctgcgcac ccagcaggtc gaggtcttcg
     5881 cgggcgtagg cgtacaccgc ctgcacgtca ccgccgtgat gcttgccgtc gccgatgtag
     5941 tcgccggcca gcccactgcg atgctcgggt cccagccgcc gggtgacagc gcgtgccccg
     6001 ggcgccgaca ccgccgccga cggcaccggt tgcttgtcga tgccggtggt gttggaacgc
     6061 ttgccgcgat cgaccgccgg gcgtccgagg ttcaccgaca gcacggacgc gctcacctcg
     6121 cacccctgac ctcgcgaggc acgaacgcca cgtcctcctc gtcgcgtgga cgcaccccga
     6181 ccgcccagcc gtgcggcgga actgtctcgg cgcagccggg gctggccagc agtacctcac
     6241 cgatcggcat cgggcccacc tcgtcagaaa tgttgagcgc cagttcgatc acctgatcgg
     6301 ggccggtcac cagcaccacc atcgactcat cggtcaggtc gcgggtggtg gcgataccgt
     6361 ccacgatcca ggggaaccgc cgccgcaggc cgatcaacga ccggtacagg tcgaaggtgt
     6421 cggcgccgaa ggggagcagg ccgtcgggtg tctgcggaaa cggcggacgc accgcgtcgt
     6481 cgcccccgat ctgatcgagc ttgatgccct cgaagcgatg ctcgtcgccg gcgtagatgg
     6541 acggcacccc gggcagcagc gtgagcagca ccgcggcgtg ctgaatgtgg cgccggtcgt
     6601 ccaacttgga cgagatgcgc gtcacgtcgt ggttgctgat gaagatctgc gggcggaagt
     6661 gctcgcagaa gcgacgatgc cgctgcaggg accagtcgag gctccacagg ttgtggtcgt
     6721 tcatcgactg ccagatgccg tgccacagct cgtactgggt gaccgagtcc agccccgaca
     6781 gctcgacgaa ctcgtccttg tcgccgtgga tcacctcgcc cagcagccag cagtcggggt
     6841 gggcggcctt gacccgctcg acgatcggac gccaggcggc cgcacccggc gcgtaggcgg
     6901 cgtccaggcg ccagccgtcg atgccgcggt ccagccagtg gctcatcacc tcgaccacga
     6961 agtcctgcac cgcagggttg gtgaagtcga gttcgaccag ttgctcgtgt ccttcgaaga
     7021 ctcgcggata gccctcgacc cagcgcagcc agtcgccctc gggcgtgccg gggccggcgg
     7081 tcagggcacg ctgcacgatc gggtgggcgc gtccgagatg gttgaacacc ccatcgagca
     7141 gaatgcggat gccgcgctcc ttcgccttcc acatcagctc gccgaaatcg ccgtcgtcgc
     7201 ccagccgcga atcgatggcg tagtggtcga gcgtgtcgta cccgtgcgtg gacgaggcga
     7261 agatcgggtt cagcagcatg ccgttgcagc ccaggtcgag cagatagtcg agccagttgg
     7321 tcaactggcc cagccggtgc cgcacctcat gaacggcggt gtcttcggcg ttgacgaagc
     7381 cgagcgggta gcaatgccac cagatgacgt gtcccgtcca gtccatcgat ctcctcacca
     7441 cacctcccgg ggagtctatc gaccggtggt cagctccgga tcgctcaggc ccagtctctg
     7501 aaagcccccc accatcgatc gggcagggcg aactcatgcg cccgtgctcg cgcttgttgc
     7561 ggccgtgttc caatcctggt cgcggggcag gtgcggcgcg aaccgcctga cacgatggac
     7621 ggcatgcaac gcttcgtcag tgcccacatc gaggccaccc tggacgttcc caccgacgtg
     7681 gtgcttgcca tcgccgttgc cgaggggccc gagcgtagcg agcagctgac catgacggcc
     7741 aacggcgcac ccgccttcgc ccgtgagatc gccgccccgc acggtggtcg gttgcacctg
     7801 gtcgagggcc tgccgcccgg accgctgacc atcgactatg gggcgagcct ggacgggctg
     7861 gccgagcccg aggtgcccac cgacctcgac cgcatcacgt atgcccggcc cagccgctac
     7921 tgcgattccg acaagctgat gccggtcgcg caggccgagt tcgcgggcct ggagggcaag
     7981 ctgttgctgg acgccgtgtc gagttgggtg ggttcgcagc tgtcgtacgt gtccggctcc
     8041 tccaagccga tcgacggggc ggtcgacacc tatctgcttc ggcagggcgt gtgccgcgac
     8101 tacgcgcacc tggtgatcgc gctgctgcgc gggctgaacg tgcccgccag gttggccgcc
     8161 gtgtatgcgc ccgggctcga cccgatggat tttcacgccg tcgccgaggc ctacgtggac
     8221 ggtgcctggt gggtggtgga cgcgacgacg ctggctccgc gtccgtcgct ggtgcggatc
     8281 gcgaccggcc gcgacgccgc ggacaccgcc tttctcagca cgctgaaggg ttcgctcacc
     8341 ctgcattcgg tgctggtcgg cgccaccgtg gacgcgctgc ccggcgacga cctgacgctg
     8401 ctcaccgccc tgcgctgagc aacgatcagt cctgaggaac gatcagcgcg ttgcgttggt
     8461 cgagcattgt caatgcgttc cgttggttga gcttggcgaa accgctgctc acaagggcga
     8521 tgacagcttc gtccagcgat agatcagcag cgcgccgagg agctctgtgg acggttgcgg
     8581 cggcggtccg cacaccgggt gcgggggccg cacgagcggc gtagccgacc cgcctcgccc
     8641 ggtggacgca ccctcgccgg ttgccactac atttgcgcgc atgactgtcg agccggacga
     8701 ccaggccagc accgaaatcg acgacgtgct gcccgagatg accttcgatg cgcagttcta
     8761 ccccgcgcgt ccgaagaagc ttcggccggc ggcccgccga cgcaacccgc tgcacgcgat
     8821 cagcgcgccg cccttcgccc cggacggccg caacaaggcc tacgtggagt ggctggtgca
     8881 gcagtccatg ctgggcgacg cccgcacgct ggcccgccag ttggccggcc tgcacaccat
     8941 gtggagcaac ccgttcgccg caccggaccc ccgcgccgcg gtcgaccggg cgtcggtctg
     9001 gttcaccgcc tacccactgt cgctggtcac ggcgcccaac cagtcgtttc tgggggccct
     9061 gggcgacgac gacctgtgga gttgcttcga gtcggtcggg gtggacgcgg tgcacacggg
     9121 cccggtcaag ctggccgggg gcattcgcgg ctgggacgcg acccccagcg tcgatggtca
     9181 cttcgaccgg atcagcatgg cgatcgaccc gctgttcggc accgaggagg agtaccgcgg
     9241 catgtgcgaa accgcgggcc ggcaccacgc gatgatcatc gacgacatcg ttccgggcca
     9301 taccggcaaa ggggcagact tccggctggc cgagctgaac tacggcgact acccgggcat
     9361 ctaccatctg gtcgacattc cgccggaggc ctggcatctg ctgcccgagg tgcccaaggg
     9421 cttcgacgcg gtgaacctcg acccgtccca tgaacgggcg ctggccgagg cgggttacat
     9481 catcggcgaa ctgcagcggg tgatcttcta cgagccgggc gtcaaagaga ccaactggtc
     9541 ggccaccccg ccgatctacg accacaccgg caccctgcgg cgctgggtgt atctgcacta
     9601 cttcaaggag ggtcagccct cgatcaactg gctcgacccc accttcgccg gcatgcgtct
     9661 ggtgatcggc gatgcgctgc actcactgct cgatctcggc tcgggcgggc tccggctcga
     9721 cgccaacggc tttctgggcg tcgagaagaa tgtgtcgggg ccggcctggt ccgagggcca
     9781 ccccttgtcg caggccgcga atcaactcat cggctcgatg gtgcgcaagg tgggcgggtt
     9841 cacctttcaa gaactcaacc tgggtatcga cgacatcgcg gagaccagca aggtggggcc
     9901 ggacctctcc tacgacttcg tcacccggcc cgcctaccag ttcgcgctgg ccaccggtga
     9961 caccgagttt ctgcggctca ccctgcgtga ggcgatggct gccggcatcg accaggcctc
    10021 gctggtgcat gccctgcaga accacgatga gctcacctac gagttggtgc atttcgccgg
    10081 ccggcatgcg cacgacacct acaccctggg cggcgtcacg tactcgggca ccgacctggc
    10141 cgagcacgtc cgcgaaaccc tgcgactgaa gatcaccggt gatgccggcc cgtacaacgc
    10201 ggtgttcacc cagaacggca tcgcgtccac caccgcgtcc gtgatcgcag cgagcctggg
    10261 ctacgacgat ctggacgcga tcggcccgtc cgaggtggag cgcatcaaga gtgcccatct
    10321 gctgctcagc atgtacaacg cgtggcagcc gggggtcttc gcgttgtcgg ggtgggatct
    10381 ggtcggcgcg ctgccgctga agcggtcgca ggtcgagagc ctgatcgcca cgggcgacac
    10441 ccgctggatc gagcgcggcg cctacgatct gatgggctgg tcacccgagg ccaccacttc
    10501 ctcgtccggc atgccgctgg cgcgggctct gtacggctcg cttccggaac agttggccga
    10561 ctccaagtcg ttcgtgtccc ggctgtcggc gatcctgaag gtgcgcaaac ggcacggctt
    10621 cttttccggc agcctgctcg acgtgcccga ggtgccgacc aaagagatgt tggtgctggt
    10681 caaccggctg ttcgtcggcg cgatccagat caccgcgctg aacttcgggc ccgagaagat
    10741 ccagggtcac gtgcagtcgg cgcagattcc gcccggacgg gtgttcgacc tcagcacccg
    10801 ccgcaagatc gacaacgtgg acgacctcgg cggtttcaac gtcaccctgc cgccgttcgg
    10861 agggctggcg atgctggtgc gcgaggggta gccggagagg gctgacccgg agccgaaaac
    10921 cctgccgcgg cgacatcgcc ccgacgatcg tgaccgatgc cgcccaccgg gcggcatcgg
    10981 tcgatcatcg ctagcagggc ggatcgtcaa ctcaaccggt cagaaacacg gcccgctgcg
    11041 cttcgaagtc ggccgtgtga acgtcaaccg gccaggatca cggccttttg agcttcgaac
    11101 tcggcatcgg tcagcacacc ggcggtgcgc agctcaccca actgggtc
//
LOCUS       DAOSUG010000023        10945 bp    DNA     linear   ENV 10-JUN-2024
DEFINITION  MAG TPA_asm: Micropruina sp. isolate AMR_MDS_2195
            NODE_3357_length_10945_cov_5.186134, whole genome shotgun sequence.
ACCESSION   DAOSUG010000023 DAOSUG010000000
VERSION     DAOSUG010000023.1
DBLINK      BioProject: PRJNA925477
            BioSample: SAMN32864262
            Sequence Read Archive: SRR4236673
KEYWORDS    WGS; ENV; Metagenome Assembled Genome; MAG; Third Party Data; TPA;
            TPA:assembly.
SOURCE      Micropruina sp. (wastewater metagenome)
  ORGANISM  Micropruina sp.
            Bacteria; Bacillati; Actinomycetota; Actinomycetes;
            Propionibacteriales; Nocardioidaceae; Micropruina.
REFERENCE   1  (bases 1 to 10945)
  AUTHORS   Abdulkadir,N., Saraiva,J.P., Zhang,J., Stolte,S., Gillor,O.,
            Harms,H. and Rocha,U.
  TITLE     Genome-centric analyses of 165 metagenomes show that mobile genetic
            elements are crucial for the transmission of antimicrobial
            resistance genes to pathogens in activated sludge and wastewater
  JOURNAL   Microbiol Spectr 12 (3), e0291823 (2024)
   PUBMED   38289113
REFERENCE   2  (bases 1 to 10945)
  AUTHORS   Abdulkadir,N.
  TITLE     Direct Submission
  JOURNAL   Submitted (24-MAR-2023) Environmental Microbiology, Helmholtz
            Center for Environmental Research -UFZ, Permoserstr 15, Leipzig
            04318, Germany
COMMENT     The annotation was added by the NCBI Prokaryotic Genome Annotation
            Pipeline (PGAP). Information about PGAP can be found here:
            https://www.ncbi.nlm.nih.gov/genome/annotation_prok/
            
            ##Genome-Assembly-Data-START##
            Assembly Method        :: SPAdes v. 3.11.1
            Genome Representation  :: Full
            Expected Final Version :: Yes
            Genome Coverage        :: Not Applicable
            Sequencing Technology  :: Illumina MiSeq
            ##Genome-Assembly-Data-END##
            
            ##Genome-Annotation-Data-START##
            Annotation Provider               :: NCBI
            Annotation Date                   :: 05/04/2023 04:57:10
            Annotation Pipeline               :: NCBI Prokaryotic Genome
                                                 Annotation Pipeline (PGAP)
            Annotation Method                 :: Best-placed reference protein
                                                 set; GeneMarkS-2+
            Annotation Software revision      :: 6.5
            Features Annotated                :: Gene; CDS; rRNA; tRNA; ncRNA
            Genes (total)                     :: 2,776
            CDSs (total)                      :: 2,750
            Genes (coding)                    :: 2,714
            CDSs (with protein)               :: 2,714
            Genes (RNA)                       :: 26
            tRNAs                             :: 24
            ncRNAs                            :: 2
            Pseudo Genes (total)              :: 36
            CDSs (without protein)            :: 36
            Pseudo Genes (ambiguous residues) :: 0 of 36
            Pseudo Genes (frameshifted)       :: 2 of 36
            Pseudo Genes (incomplete)         :: 32 of 36
            Pseudo Genes (internal stop)      :: 2 of 36
            ##Genome-Annotation-Data-END##
FEATURES             Location/Qualifiers
     source          1..10945
                     /organism="Micropruina sp."
                     /mol_type="genomic DNA"
                     /submitter_seqid="NODE_3357_length_10945_cov_5.186134"
                     /isolate="AMR_MDS_2195"
                     /isolation_source="Activated sludge of municipal
                     wastewater treatment plants"
                     /db_xref="taxon:2737536"
                     /environmental_sample
                     /geo_loc_name="Canada"
                     /lat_lon="49.25 N 123.1 W"
                     /collection_date="2015-06-16"
                     /metagenome_source="wastewater metagenome"
                     /note="metagenomic"
     gene            complement(<1..639)
                     /locus_tag="PLF56_01500"
     CDS             complement(<1..639)
                     /locus_tag="PLF56_01500"
                     /inference="COORDINATES: protein motif:HMM:TIGR02050.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_function: GO:0016881 - acid-amino acid ligase activity
                     [Evidence IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="YbdK family carboxylate-amine ligase"
                     /protein_id="HOQ52270.1"
                     /translation="MGVTFGVEEELLVVEPRQGRPLAAGDTVVQHALDALDDASPYPE
                     DAIEHEFKREQAELGSMPCTSADELTHQLVELRAAVAAGAANSGAAVAAIGTSPFKVR
                     PTATDDERYARMTAEFAHLARQQLTCGQHVHVAIDSRDEGAAVIDRIAAWLPLLLALS
                     SNSPFWQGHDTGYASFRTVIWGLWPTAGPSGPFGDAAGYDAAVADLVRSGAAL"
     gene            complement(892..1125)
                     /locus_tag="PLF56_01505"
     CDS             complement(892..1125)
                     /locus_tag="PLF56_01505"
                     /inference="COORDINATES: ab initio
                     prediction:GeneMarkS-2+"
                     /note="Derived by automated computational analysis using
                     gene prediction method: GeneMarkS-2+."
                     /codon_start=1
                     /transl_table=11
                     /product="hypothetical protein"
                     /protein_id="HOQ52271.1"
                     /translation="MADLSTEKREDLPAKDFGLPEKARTKDAKKDSGNYPMPDKAHAR
                     NAKSRAAQQQKAGNLTEKEKERIDRKADKKLDD"
     gene            complement(1518..1775)
                     /locus_tag="PLF56_01510"
     CDS             complement(1518..1775)
                     /locus_tag="PLF56_01510"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_013864248.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="type II toxin-antitoxin system RelE/ParE family
                     toxin"
                     /protein_id="HOQ52272.1"
                     /translation="MTHRIELRPAAVRALGKVEPGARGRIQGAIALLAENPRPPGARA
                     LHGRPGLRVRVGDYRIIYLVEDDVLLVVVVTPGHRRDVYHR"
     gene            complement(1772..2023)
                     /locus_tag="PLF56_01515"
     CDS             complement(1772..2023)
                     /locus_tag="PLF56_01515"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_013864249.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_component: GO:0110001 - toxin-antitoxin complex
                     [Evidence IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="type II toxin-antitoxin system Phd/YefM family
                     antitoxin"
                     /protein_id="HOQ52273.1"
                     /translation="MASTMSVADARNSFAEALERSGEGPVFIERRGRRAAVLISPSEY
                     ERLIDAVEEQEDVTAFDEAMADEGPNIPWSQVKVDLGWA"
     gene            complement(2045..2314)
                     /locus_tag="PLF56_01520"
     CDS             complement(2045..2314)
                     /locus_tag="PLF56_01520"
                     /inference="COORDINATES: ab initio
                     prediction:GeneMarkS-2+"
                     /note="Derived by automated computational analysis using
                     gene prediction method: GeneMarkS-2+."
                     /codon_start=1
                     /transl_table=11
                     /product="hypothetical protein"
                     /protein_id="HOQ52274.1"
                     /translation="MLPQLTERERDVSNLLAGGLSNAATDRRPNIAPKSVGQPHFSAI
                     LLTLGVATFTDAIVMSGNAALGRHAPMKKPGPSAWHGRSSVMYTK"
     gene            complement(2592..2813)
                     /locus_tag="PLF56_01525"
     CDS             complement(2592..2813)
                     /locus_tag="PLF56_01525"
                     /inference="COORDINATES: ab initio
                     prediction:GeneMarkS-2+"
                     /note="Derived by automated computational analysis using
                     gene prediction method: GeneMarkS-2+."
                     /codon_start=1
                     /transl_table=11
                     /product="hypothetical protein"
                     /protein_id="HOQ52275.1"
                     /translation="MTNRGLYAIPDARAGTTTTNAGGPRGKPAMTTGPWRVRGRNNLF
                     GSSYPGAGATIAQGLVFGQIIAEHAAQQA"
     gene            3627..5402
                     /locus_tag="PLF56_01530"
     CDS             3627..5402
                     /locus_tag="PLF56_01530"
                     /inference="COORDINATES: protein motif:HMM:NF014741.2"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="DUF222 domain-containing protein"
                     /protein_id="HOQ52276.1"
                     /translation="MENSFEHHDDHDLVVTLDRVLDALTDDRLRLAGPADRLKLALAA
                     VRVASRVSVWAQELVAGIDSDEVAVQVHGTSTTTWLTDAARFTPREARRLIRAGEGLA
                     RFPQVSQAARAGEVSAVQAEAITGVLDDLPADFDTEQLAQGEALMVEFAHTHNSRELR
                     MLSQHLVEVLSPETAEQSEADRLERELKGATARRCLAFVDDGHGSVLIRGSLPVADAE
                     PFRKLIDSYAAQVKRGLEALDPLQPTMTATQRRADALVRLVDRHQQQSLAPCLGGDRP
                     RVTMTVSWETLLRQARETGQPGGADDTGADCRGDEAAGAGHATDGHAAAATGAAAPTE
                     DGAASTDGAAAGDGHAAAATGAAASTEDGAAATEAAASTDGAAAGDGHAAVATGAAAS
                     ADGAAATDTNIDGPTGSSASTDARPRSDGGGATCSRPTSDTEQTSHASPGAGRTGTGQ
                     STAPSVLRRLLCDADILPIVLGGPSEILDVGREQRLVTAAIRAALNQRDQGCVFPGCD
                     TPPDACHAHHIQPWWNGGTTSLGNLVLLCPHHHGLIEPSHDPSADRWSLHLRPDGVSE
                     IRPPRRVDPRQHPRIHARFLTHLRT"
     gene            complement(5708..5875)
                     /locus_tag="PLF56_01535"
     CDS             complement(5708..5875)
                     /locus_tag="PLF56_01535"
                     /inference="COORDINATES: ab initio
                     prediction:GeneMarkS-2+"
                     /note="Derived by automated computational analysis using
                     gene prediction method: GeneMarkS-2+."
                     /codon_start=1
                     /transl_table=11
                     /product="hypothetical protein"
                     /protein_id="HOQ52277.1"
                     /translation="MTITKELPSQANTQTDLVDHGWSTLVPSGHDRKAGVSDEVIAFA
                     QASQPKEWQSW"
     gene            complement(5884..6966)
                     /locus_tag="PLF56_01540"
     CDS             complement(5884..6966)
                     /locus_tag="PLF56_01540"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_017577110.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="Fic/DOC family N-terminal domain-containing
                     protein"
                     /protein_id="HOQ52278.1"
                     /translation="MTWAAGVPHNDLPALPPHGVDLEPKPVLKAAIEARAAVASLAQA
                     GNLLPNPGVLVHAVPLLEAQASSEIENIATTADELFKHAESGGGDQATKEALRYRNAL
                     FAGLRAIRERPLTAATAAQICSALHGHEVDVRAVPGTRIANPATRQVIYSPPESRDLI
                     RDKLSEWEKFIHSNDDLDPLVRMALAHYQFEAIHPFHDGNGRTGRVLNILMLIEAGLL
                     SEPILYLSRAIIETKDDYYRLLLAVTAEGAWTDWIVYMLEAVRSSAESTQRKIGSIRT
                     CQNEIAERARTASPGGRDARFLKVLSEQPYCRINMVAERCEVSRQTASGWLHALVSSG
                     PFTDLKVGREVLFVNHGFLDVLTRPE"
     gene            complement(7207..7419)
                     /locus_tag="PLF56_01545"
     CDS             complement(7207..7419)
                     /locus_tag="PLF56_01545"
                     /inference="COORDINATES: ab initio
                     prediction:GeneMarkS-2+"
                     /note="Derived by automated computational analysis using
                     gene prediction method: GeneMarkS-2+."
                     /codon_start=1
                     /transl_table=11
                     /product="hypothetical protein"
                     /protein_id="HOQ52279.1"
                     /translation="MSRQGFHDKVAFVWKVADKLRGIFKQHEYGSVILPLHKQSLIAA
                     AVTGGPTPRPPHTPRRSMSRTRLAHG"
     gene            complement(7541..7957)
                     /locus_tag="PLF56_01550"
     CDS             complement(7541..7957)
                     /locus_tag="PLF56_01550"
                     /inference="COORDINATES: protein motif:HMM:NF013970.2"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="PIN domain-containing protein"
                     /protein_id="HOQ52280.1"
                     /translation="MQFVDTNVLLYAISTEPAEAEKARVAVEILSRAELGLSTQVLQE
                     FYVQATRATRSARLSHEQATDLVEAFTRFTVQPVTVEIVRAALNLRGRHSLSYWDAAI
                     IEAARTLRCTEILSEDLSADENYDGLRVINPFTTTD"
     gene            complement(7944..8183)
                     /locus_tag="PLF56_01555"
     CDS             complement(7944..8183)
                     /locus_tag="PLF56_01555"
                     /inference="COORDINATES: ab initio
                     prediction:GeneMarkS-2+"
                     /note="Derived by automated computational analysis using
                     gene prediction method: GeneMarkS-2+."
                     /codon_start=1
                     /transl_table=11
                     /product="hypothetical protein"
                     /protein_id="HOQ52281.1"
                     /translation="MKNITVTVPDEVYLQARIRAAQSGTSVSALVTRYLQSLTDSDAE
                     FRRLAELQDRVKADIRTFSAGDRLDREAVHERAVR"
     gene            complement(8238..9602)
                     /gene="dnaB"
                     /locus_tag="PLF56_01560"
     CDS             complement(8238..9602)
                     /gene="dnaB"
                     /locus_tag="PLF56_01560"
                     /EC_number="3.6.4.12"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_013865955.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_function: GO:0003678 - DNA helicase activity [Evidence
                     IEA];
                     GO_process: GO:0006260 - DNA replication [Evidence IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="replicative DNA helicase"
                     /protein_id="HOQ52282.1"
                     /translation="MSIASLPPYEEDPRDFGPSDRTPPQDVQAEQSVLGAMLLSKDAI
                     GDVAEVVRGRDFYLPKHEQIYDAIVDLFGRGEPADAITVADELAKRGELGKVGGHIYL
                     HDLLQMVSIASNAGYYAEIVKEKAILRRLVEASIKISQMGYAGGGEVASIVDQAQQAI
                     YEVAEGKTSDDYEPLSVLMERTLDEIESLSQHGVMAGVPTGFADLDELTNGLHAGQMI
                     IIAARPGMGKSTLGLDLARAASIKNKLTSCFFSLEMGKSEIVQRLISAEAGVPLNHIR
                     KGQMGDDDWQRIARKTGEVSSAPLFIDDSPNLTMMEIRAKARRLKQRHDLKLVIVDYM
                     QLMTSGKKVESRQLEVSEFSRQMKLLAKELEVPVVALSQLNRGPEQRTDKKPMLSDLR
                     ESGSLEQDADMVILLHRADAYDKDSDRVGEADFIVAKHRNGPTATIGTVFQGHYSRFV
                     DMQH"
     gene            10163..10507
                     /locus_tag="PLF56_01565"
     CDS             10163..10507
                     /locus_tag="PLF56_01565"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_020577217.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="metal-sensitive transcriptional regulator"
                     /protein_id="HOQ52283.1"
                     /translation="MSHVHHETPAQGADNTPHAALSHGYLSSKDDYLKRLRRIEGQVG
                     GLAKMVSDERYCIDILTQISAATKALESVALGLLDEHLRHCVVRAAREGGDEAETKLA
                     EAHDAIARLVRS"
     gene            10530..10733
                     /locus_tag="PLF56_01570"
     CDS             10530..10733
                     /locus_tag="PLF56_01570"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_008355452.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_function: GO:0046872 - metal ion binding [Evidence
                     IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="heavy-metal-associated domain-containing
                     protein"
                     /protein_id="HOQ52284.1"
                     /translation="MQTTYTVEGMTCGHCVAAVTEEVKLIPGVTDAEVVLEGGRLTIT
                     SADPVDFDRVVEAVAEAGDYTVA"
BASE COUNT         1781 a         3630 c         3648 g         1886 t
ORIGIN      
        1 gagtgcggcg cccgaacgca ccagatccgc gaccgccgcg tcgtagccgg cggcgtcgcc
       61 gaacggtccg gacggtcccg cggtcggcca caacccccag atgacggtcc gaaaactcgc
      121 gtaccccgtg tcgtgaccct gccagaacgg cgagttggac gagagtgcca gcagcaacgg
      181 caaccaggcc gcgatgcggt cgatcacggc ggcaccctcg tcccgcgaat cgatcgcgac
      241 gtgcacgtgc tgcccgcagg tcaactgctg gcgggccaga tgggcgaact cggccgtcat
      301 ccgtgcgtag cgctcgtcgt ccgtcgcggt cggacgcacc ttgaacgggc tggtgccgat
      361 cgccgcgacc gccgcgcccg agttggcggc cccggcggcg acggccgctc gcaactcgac
      421 caactgatgc gtcagttcgt cggcggaggt acagggcatc gagccgagct cggcctgttc
      481 gcgcttgaac tcgtgctcga tggcgtcctc ggggtagggc gaggcgtcgt cgagcgcgtc
      541 cagggcgtgc tgaacgaccg tgtcgccggc cgcgagtgga cgcccctgcc ggggttcgac
      601 cacgagcagt tcctcttcga ctccgaacgt cacacccatg cgcacagtct ggcccgtcgc
      661 gccgcccaac ggatccaccg ccccgccttc cgtgctggtg tgtgtggagg gctgcctgat
      721 cggcccgaga gatgccgggg ccgcgccacc ttgaggtata gccgcgaacg ccatgccggg
      781 ccgcttgacc gcccaccagc agcaacggac gcacccgctc cagtcgctcc gcgcctcggc
      841 ctcatggtta cggcaggccg agcgtccgag ttgatgagcc gcggcggacg gtcagtcgtc
      901 gagcttcttg tcggccttgc ggtcgatgcg ctccttctcc ttctcggtca ggttgcccgc
      961 cttctgctgc tgcgcggcgc gcgatttcgc gttgcgggcg tgtgccttgt cgggcatcgg
     1021 gtagttgccg gagtccttct tggcatcctt ggttcgagcc ttctcaggca gtccgaaatc
     1081 cttggcgggc aggtcttcgc gcttctcggt agagagatcg gccatggcgc acctcgaggg
     1141 ctggggccgg gctggtcccg gcgcggtcgc cgccacgcta gccaccggat cggacgtgcg
     1201 ataccgcctt ttggtcggca cccgatgacc caatcgccac aaccggacgc gcggtcggcg
     1261 tccgagtcgg cggattcaag acgcggtctc ggaatcgact ccttaccgag cggttctcgg
     1321 ccgcttggcc gcctgttgcg ccctccaccg caccacgcgt accgaccggc ggtgggtgct
     1381 gaatcagggc tgcggggcgc gcggccctga cggagacgcc gcggcgtggt ctggcgtccg
     1441 gtcgggggga cggtgcgtca tccgcaggcc gcctgctgcg ggtgcggcgt gcttggggtc
     1501 gaagtcgctg cagagcgtca gcggtgatag acgtcacgcc gatggccagg ggtgaccaca
     1561 accacgagca gtacgtcgtc ttcgaccagg tagatgatgc ggtagtcgcc gacgcgaact
     1621 cgaagcccgg gccgtccgtg caacgcacgt gcgcccggcg gccggggatt ctcggccaga
     1681 agtgcgatcg ccccctgaat gcgcccgcgt gccccaggct cgaccttgcc cagtgcccgg
     1741 acggccgccg gccgcaactc gatgcggtgg gtcatgccca gccgagatcg accttgacct
     1801 ggctccaggg gatgttcggc ccctcgtccg ccatcgcctc gtcgaacgcg gtgacgtctt
     1861 cctgctcctc cacggcgtcg atgaggcgct catattcact cgggctgatc agaacggccg
     1921 cccggcgtcc ccgtcgttcg atgaagaccg ggccctcacc ggagcgctcg agtgcttcgg
     1981 caaaactatt gcgggcgtcc gcaacgctca tggtgctggc catggatcca atgtacaaga
     2041 ggcatcactt tgtgtacatg acggatgatc gtccgtgcca cgctgacggt ccgggcttct
     2101 tcataggggc gtgacgcccg agcgcggcgt tgcccgacat gacgatcgcg tccgtgaacg
     2161 tcgcgacgcc gagcgtgaga aggatcgcgg agaaatgtgg ttgtcccaca ctcttgggag
     2221 cgatgttcgg tcgccggtca gtcgccgcgt tggagaggcc gccggccagc aggttgctca
     2281 cgtcgcgctc gcgctcggtc agctggggaa gcactcttgg ggacggtgcc gggtcgaccg
     2341 agcgacgaag cacccggcgg tcgaccccgt gaccgtcgcc cgcgtcgctc acgtcgacgg
     2401 cgtcgctcgc ctcacccgtc tcgcccgcgg cgaccgcagg gcgcgttcga atcctggtgt
     2461 tcggcgcctt gagcaggtag ccgtgggcgg cgagggcgtc cgcaacctgc ggctggaggc
     2521 tgaaactgtg aatcccccgg ggacggcagg tgtgccgtcc gccgggggat ccaggtgtgg
     2581 gagcgtgcgg ctcaggcctg ctgggcggcg tgctcggcga tgatctggcc gaaaaccaga
     2641 ccctgtgcga tcgtcgcccc ggcgcccggg tagctgctgc cgaacaggtt gttgcggccc
     2701 cggacgcgcc aaggtccggt cgtcatcgca ggcttgcccc ggggtccccc ggcattcgtg
     2761 gtggtggtcc cggcacgggc gtcgggaatc gcgtagagac cgcggttcgt catcccgggc
     2821 tcgtcgcgga cgcgcagagg tccccggtcg tcatcccggg tttgtccccg atgcgcagag
     2881 gtccccggtc gtcatcccgg gttggtcccg gacgcacaga ggttcccggg tcgtcatccc
     2941 gggtttgtcc gacaagctcc ggcgtcgcat tggtggtcct cggcgcacgt tgggatcgtg
     3001 tcgagactcc ggccgtcgtc ccgggcttgt cccgggagcc caggcagcca gccgagttgt
     3061 catcccggct tgtcccggac gcgcagaggt ccccagccat aatcccgtct tgtcccggga
     3121 tccgggtgtt cacattggtg gctcccggcg cacgttcgga tcgtgccgag actccgtatc
     3181 attccgggct tgcttcggaa taccaggcaa ccggccgagt cgtcatcccg ggttgccccg
     3241 gggtcccagg cagccagccg agtcatcgtc cccgggcttg tagcggacgc gccgactccg
     3301 gatcgttatc ccgggtttgt gccgggatca tggcgttcac gttggtggtt ctcggcgcag
     3361 cttgggatcg tgtcgagacc gcgagtcgtc gtccctggct tgcccgggat ctccggcgtt
     3421 cacgttggac gtccaggcgc acgttgggat cgtgtcgaga ctccgagccg tcgtccgggg
     3481 cttgtcccga gagcccaggc agccagccga cgggatcgct caagcggacg attgcaattt
     3541 gtgaggaata atggaaaccg ctgccgcttg tggatacgtg gttggccgtt cgaaaagtgt
     3601 cagagcctgt gtctagcgtg tgatgcatgg agaactcgtt cgagcatcac gacgaccacg
     3661 acctggtcgt cacgctcgat cgcgttctcg acgccctgac cgacgaccga cttcggctgg
     3721 ctggcccggc cgacaggttg aaactggcgc tggccgcggt gcgggtcgcc tccagggtca
     3781 gcgtctgggc gcaagagttg gttgccggca tcgactccga cgaagtagcc gtccaggtgc
     3841 acggcacttc gaccaccacc tggctcaccg acgcggcccg tttcaccccg cgtgaggcgc
     3901 gtcggttgat ccgtgccggg gaaggcctgg cccggttccc gcaggtgagt caagcggcac
     3961 gcgccggcga ggtgtcagcg gtccaagccg aagcaatcac cggcgttctg gacgacctgc
     4021 cggccgactt cgacaccgaa caactggccc aaggtgaagc actcatggtc gagttcgccc
     4081 acacccacaa cagtcgtgag ctacgaatgc tgtcgcagca tctggtggag gtgctcagcc
     4141 ccgagacagc ggagcagtcc gaagccgacc ggttggaacg ggaactgaaa ggcgcgacag
     4201 cccgccggtg cctggccttc gtcgatgacg ggcacgggtc ggtgctgatc cgaggcagcc
     4261 tgccggtggc tgatgccgaa ccgttcagaa aactgatcga cagctacgcc gcccaagtca
     4321 aacgcggcct cgaagccctc gacccgttgc agcccaccat gacggcgacg cagcgcaggg
     4381 ctgacgcgct ggtccggctg gtggatcggc accagcaaca gtccttggct ccctgcctgg
     4441 gcggcgaccg gccacgcgtg acgatgaccg tctcctggga gacgttgctc cggcaggccc
     4501 gtgagaccgg gcagcccggc ggcgcagacg acaccggagc ggactgccgc ggcgacgagg
     4561 ccgcaggcgc cggtcacgcc accgacgggc acgctgctgc agccaccggc gccgcagcac
     4621 ccaccgagga tggcgctgca tccaccgatg gtgctgcggc tggcgacggg catgctgctg
     4681 cagccaccgg tgctgccgca tccaccgagg atggcgctgc ggccaccgag gccgccgcat
     4741 ccaccgatgg tgctgcggct ggcgacgggc atgctgctgt agccaccggt gctgccgcat
     4801 cggccgatgg tgctgcggcc accgacacga acatcgacgg gcctaccggc tcgtccgcct
     4861 ccacagatgc tcgcccgcgc agcgacggcg gcggggcaac ctgctcgcgc ccgacaagcg
     4921 acaccgagca gaccagccac gccagccccg gcgcaggccg gaccggcacc gggcagagca
     4981 ccgcacccag cgtgctgcgc agactgttgt gcgacgccga catcctgccc atcgtgctgg
     5041 gcgggccgtc cgagatactc gacgtcggcc gggagcaacg ccttgtcacc gccgccatcc
     5101 gcgccgcgtt gaatcaacgc gaccagggct gtgtgttccc aggttgcgac accccaccgg
     5161 acgcctgcca cgcccatcac attcagccct ggtggaatgg cggcaccacc tcactgggca
     5221 acctcgtcct gctctgtccg caccaccacg gcctgatcga acccagccac gacccgtccg
     5281 ccgaccggtg gagcctgcac ctgcgccccg acggggtcag tgaaatccgg ccaccccgtc
     5341 gggtcgaccc caggcaacac ccccgcattc acgcgcgatt cctcacccac ctcagaacct
     5401 gatggcaccc gcccccggtg aaggccaccc aacccgctac gtcgagtcac caaaccaggg
     5461 aaggccgtgc cttcagcgcc gcctcgcgtc gtaggtaccg gcgaggtagc gcagaagcaa
     5521 ggcctgagac tcgcgtttgg cgaagtacaa gtcgaacaga gtggcgggct tggaagagca
     5581 cagccgcacg ttcacgccga gtcctgcacc ggggaccgca gcacgctgat cgggccgcga
     5641 cgatccaggg cgtcgaccac caccttgagg aacttctccc ggggcgccgc ctcgccaccg
     5701 tgccgggtca ccagctctgc cactccttcg gctgcgaggc ctgcgcgaac gcaatcacct
     5761 catccgaaac gccggccttg cggtcgtggc cgctcggcac cagtgtgctc caaccgtggt
     5821 cgaccaggtc cgtctgggtg ttcgcctgcg aaggcagttc tttggtgatg gtcatggggg
     5881 tggttactcc ggtcgggtga ggacgtcgag aaatccatgg ttcacgaaca ggacctcgcg
     5941 gccgaccttg aggtcggtga aggggccgct ggagacgagg gcgtgcagcc agccggaggc
     6001 cgtctgacga gacacctcgc aacgctccgc caccatgttg atccggcaat agggttgctc
     6061 ggagagcacc ttgaggaagc gggcgtccct tccaccgggc gaggccgtcc gggcgcgttc
     6121 ggcgatctcg ttctggcagg tccggatgga tccaatcttg cgttgggtcg attcggccga
     6181 tgagcggacc gcctccagca tgtagacgat ccagtcggtc cacgcgccct cggccgtcac
     6241 cgccagcagt agccggtagt agtcgtcctt ggtctcgatg atggctcgcg acagatagag
     6301 aatcggctcg ctcaacaacc cggcctcgat caacatcagt atgttgagca cgcgcccggt
     6361 gcggccgttg ccgtcatgaa atgggtggat cgcctcaaac tggtagtgcg ccaaggccat
     6421 gcgcaccagc gggtcgagat cgtcattgga gtggatgaac ttctcccact ctgacagctt
     6481 gtcgcggatc agatcgcgac tctcgggtgg gctgtagatc acctgccgag tggcggggtt
     6541 ggcgatccgc gtacctggga cggcgcgcac gtccacctcg tgaccgtgca gggcagagca
     6601 aatctgtgcg gccgtggcgg ccgtcaatgg acgctcgcgg atcgcccgca gccccgcgaa
     6661 cagggcgttt cgatagcgca gcgcctcttt ggtggcctgg tcgccgccgc cggattctgc
     6721 gtgcttgaac agttcgtcag cagtggtggc gatgttctcg atctcagagc tggcctgagc
     6781 ctccaacagc gggaccgcgt gaaccaagac gcccgggttg ggcagcaggt tgccggcctg
     6841 cgccagggac gccacggcag cccgagcctc gattgcggcc ttgaggacgg gtttcggctc
     6901 taggtcgacg ccatggggcg gcagcgcggg caagtcgttg tgagggacgc cggcagccca
     6961 agtcatatgt caccgttccg gacacgtctc ggcatcgcgt taagtattgc tcgaaaacct
     7021 aacatattga tgattgactt ggaagcgctc ggacgaccag gtggtggccg gtggatcacg
     7081 gatgatccgc atggatcaac tgtgatccat gcaggcgtcg tccgatctgt taggtcagcg
     7141 gacacgctct gatgaaatgc taaccaaacc gacgagggct aacgggctaa ccggggctaa
     7201 ccggggctaa ccgtgggcta accgtgttcg gctcatactg cggcggggtg tgtgcggcgg
     7261 tcgtggcgtc ggcccgccgg tgacggcggc ggcgatcaag gactgcttgt gcaacggcag
     7321 aatcaccgag ccgtactcgt gctgcttgaa gatgccgcgg agcttgtcgg cgaccttcca
     7381 gacgaaggcg accttgtcgt ggaatccttg ccgtgacatg catcccattc aacgcgctcg
     7441 gccgtcggta cgtctgtgtg gcggcggcgc ccagcccttt gatcgtagct tctggggtgg
     7501 gaggtggggc cgccgctccc acgctcatcg ctgcgtccga tcagtccgta gtggtgaacg
     7561 ggttgatcac gcggaggccg tcgtagttct cgtcggcact caaatcctcg gacaggatct
     7621 cggtgcacct gagggtgcgg gcggcttcga tgatggccgc gtcccagtac gagaggctgt
     7681 gccgtccccg cagattcaat gctgctcgca cgatttcgac ggtcaccggc tgcacggtga
     7741 accgcgtgaa ggcctccacc aggtcggttg cctgttcgtg gctcaatcga gcgctgcggg
     7801 tagcccgggt cgcctgcacg tagaactcct ggagcacctg tgtcgacagg ccgagttcgg
     7861 cgcgcgagag aatctcgacg gctacacgcg ccttctcggc ttcggccggt tcagttgaga
     7921 tcgcgtagag aaggacgttc gtgtcaacga actgcacgct cgtgcaccgc ctcacggtca
     7981 agacgatcgc ccgcactgaa ggtgcgaatg tctgctttca cccgatcctg cagctcagcc
     8041 agacggcgga actccgcgtc tgagtcggtc agcgactgta gatacctcgt gaccagcgcg
     8101 ctcaccgacg taccactctg tgctgctcgg atgcgcgcct gcagatagac ctcgtcgggg
     8161 acggtaacag tgatgttctt cacagaaaca cagtaacgca gtcacacggt attggggcac
     8221 tggaaggcaa cagcaggtca atgctgcatg tcgacaaatc ggctgtagtg cccctggaag
     8281 accgttccga tcgtcgccgt cggcccgttg cggtgcttcg ccacgatgaa gtccgcctcg
     8341 ccgacccggt cggagtcctt gtcgtaggcg tcggcgcggt gcagcagaat gaccatgtcg
     8401 gcgtcctgct ccagcgaacc cgattcacgc aggtcggaca gcatcggctt cttgtcggtg
     8461 cgctgctcgg ggccacggtt gagctgcgag agcgccacca ccggcacctc gagctctttg
     8521 gcgagcagct tcatctggcg gctgaactcg gacacctcga gctggcgcga ctcgaccttc
     8581 ttgcccgagg tcatcagctg catgtagtcg acgatcacca gcttgaggtc gtggcgctgc
     8641 ttgagccggc gggccttggc ccggatctcc atcatggtca ggttcggtga gtcgtcgatg
     8701 aataggggag cgctgctgac ctcgccggtc ttgcgggcga tgcgctgcca gtcgtcgtcg
     8761 cccatctggc ccttgcgaat gtggttgagc ggcacgccgg cttcggccga gatcagccgc
     8821 tgcacgatct cggacttgcc catttcgagc gagaagaagc aactggtcag cttgttcttg
     8881 atggacgccg cccgggccag gtcgagcccc agcgtcgact tacccatgcc cggacgggcc
     8941 gcgatgatga tcatctggcc ggcgtgcagg ccgttggtga gctcgtccag atcggcgaag
     9001 ccggtgggca cccccgccat tacgccgtgc tggctgagcg actcgatctc gtccagtgtg
     9061 cgctccatca gcacactcag cggctcgtag tcgtcggagg ttttgccctc ggccacctcg
     9121 tagatggcct gctgcgcctg gtcgacgatg ctggccacct cgccgccgcc ggcgtagccc
     9181 atctggctga tcttgatcga ggcctcgacc agtcggcgca ggatcgcctt ttctttgacg
     9241 atttcggcgt agtagccggc gttcgaggcg atggagacca tctgcagcag gtcgtgcagg
     9301 tagatgtgcc cgccgacctt gccgagctcg ccgcgcttgg ccagctcgtc ggccaccgtg
     9361 atggcgtcgg ccggctcgcc gcggccgaac aggtcgacga tggcgtcata gatctgctcg
     9421 tgcttgggca gatagaagtc gcggccccgg accacctcgg cgacgtcgcc gatcgcgtct
     9481 ttgctgagca gcatggcacc gagcacggac tgctcggcct ggacgtcctg cggcggggtg
     9541 cgatccgacg ggccgaagtc gcggggatcc tcttcgtagg gggggagcga agcgatggac
     9601 atggaccctc ccctcagcac gacgattcct acaccggcac gctagagtcc gccaccgaca
     9661 gttctgacac tgcccgcgca aaagcaccgc cggcacggcc gacgaccggg tattcgtaag
     9721 tcgatctggt gttcgaaagt tgactatctt caggtgcggc gcagggcgtc ctgcgctgcg
     9781 taccgctcga ccatagaccc agccgaacgc acccgtccaa caggagttat ccacaggttt
     9841 tgattgtggt tgtggaacct gtggaggcac gccgtgtgcc ctgtgcacga cgcgccgagg
     9901 tagcggtgga tgagaaaaat ctgtgtcacc acacccgcca ttcactaggg cttttaatct
     9961 gttgttcata cgagtttcat gcggtgggcg acacgccgaa gccgaatggg actggcttga
    10021 caccgcacgc aacccgtggt gtatgcgctt tcatcgacgg gttgtccaca tgtgtccgcg
    10081 caacatccgc agcgccctac atacccatag ggggtatact gacaagggcg aacgcgatgc
    10141 gtcgcacgaa ggggaggaac acatgtcaca cgtgcaccac gaaacaccgg cgcagggagc
    10201 tgacaacacc ccccacgcgg ccctctcgca cggctatctc agtagcaagg acgactacct
    10261 caagcggctg cgccgtatcg aggggcaggt gggcgggctg gccaagatgg tgtccgatga
    10321 gcggtactgc atcgacatcc tgacccagat ctcggcggcc acgaaggcgc tggagtcggt
    10381 cgcccttggg ttgctcgatg agcacctgcg gcactgcgtc gtccgtgccg cccgcgaggg
    10441 tggcgatgag gccgagacca agctcgccga ggcgcacgac gcgatcgcac gcctcgtccg
    10501 ttcctgacag cacaacccaa ggagaaacca tgcagaccac ctacaccgtc gaaggcatga
    10561 cctgcggcca ctgcgtcgcg gccgtcaccg aagaggtcaa gctgattccg ggcgtcaccg
    10621 acgccgaggt cgtcctcgag ggcggccggc tgaccatcac ctccgccgac ccggtcgact
    10681 tcgaccgagt ggtcgaggcc gtcgccgagg ccggcgacta caccgtcgcc tgagcgaacc
    10741 gccgaaaggt gcgcgccagt ggccgcccgc ccggcgtgcc gcgcggaagt cggcgcactg
    10801 gcgcgcacct ttcggacgat ttgggagaga acagtaaatg tccaccgcag gacacacctc
    10861 gatcgaactc gacatcgacg gcatgacctg tgccagctgc gccgcccgga tcgagaagaa
    10921 actcaacaag gtggacggcg tcacg
//
LOCUS       DAOSUG010000024        10931 bp    DNA     linear   ENV 10-JUN-2024
DEFINITION  MAG TPA_asm: Micropruina sp. isolate AMR_MDS_2195
            NODE_3365_length_10931_cov_4.610151, whole genome shotgun sequence.
ACCESSION   DAOSUG010000024 DAOSUG010000000
VERSION     DAOSUG010000024.1
DBLINK      BioProject: PRJNA925477
            BioSample: SAMN32864262
            Sequence Read Archive: SRR4236673
KEYWORDS    WGS; ENV; Metagenome Assembled Genome; MAG; Third Party Data; TPA;
            TPA:assembly.
SOURCE      Micropruina sp. (wastewater metagenome)
  ORGANISM  Micropruina sp.
            Bacteria; Bacillati; Actinomycetota; Actinomycetes;
            Propionibacteriales; Nocardioidaceae; Micropruina.
REFERENCE   1  (bases 1 to 10931)
  AUTHORS   Abdulkadir,N., Saraiva,J.P., Zhang,J., Stolte,S., Gillor,O.,
            Harms,H. and Rocha,U.
  TITLE     Genome-centric analyses of 165 metagenomes show that mobile genetic
            elements are crucial for the transmission of antimicrobial
            resistance genes to pathogens in activated sludge and wastewater
  JOURNAL   Microbiol Spectr 12 (3), e0291823 (2024)
   PUBMED   38289113
REFERENCE   2  (bases 1 to 10931)
  AUTHORS   Abdulkadir,N.
  TITLE     Direct Submission
  JOURNAL   Submitted (24-MAR-2023) Environmental Microbiology, Helmholtz
            Center for Environmental Research -UFZ, Permoserstr 15, Leipzig
            04318, Germany
COMMENT     The annotation was added by the NCBI Prokaryotic Genome Annotation
            Pipeline (PGAP). Information about PGAP can be found here:
            https://www.ncbi.nlm.nih.gov/genome/annotation_prok/
            
            ##Genome-Assembly-Data-START##
            Assembly Method        :: SPAdes v. 3.11.1
            Genome Representation  :: Full
            Expected Final Version :: Yes
            Genome Coverage        :: Not Applicable
            Sequencing Technology  :: Illumina MiSeq
            ##Genome-Assembly-Data-END##
            
            ##Genome-Annotation-Data-START##
            Annotation Provider               :: NCBI
            Annotation Date                   :: 05/04/2023 04:57:10
            Annotation Pipeline               :: NCBI Prokaryotic Genome
                                                 Annotation Pipeline (PGAP)
            Annotation Method                 :: Best-placed reference protein
                                                 set; GeneMarkS-2+
            Annotation Software revision      :: 6.5
            Features Annotated                :: Gene; CDS; rRNA; tRNA; ncRNA
            Genes (total)                     :: 2,776
            CDSs (total)                      :: 2,750
            Genes (coding)                    :: 2,714
            CDSs (with protein)               :: 2,714
            Genes (RNA)                       :: 26
            tRNAs                             :: 24
            ncRNAs                            :: 2
            Pseudo Genes (total)              :: 36
            CDSs (without protein)            :: 36
            Pseudo Genes (ambiguous residues) :: 0 of 36
            Pseudo Genes (frameshifted)       :: 2 of 36
            Pseudo Genes (incomplete)         :: 32 of 36
            Pseudo Genes (internal stop)      :: 2 of 36
            ##Genome-Annotation-Data-END##
FEATURES             Location/Qualifiers
     source          1..10931
                     /organism="Micropruina sp."
                     /mol_type="genomic DNA"
                     /submitter_seqid="NODE_3365_length_10931_cov_4.610151"
                     /isolate="AMR_MDS_2195"
                     /isolation_source="Activated sludge of municipal
                     wastewater treatment plants"
                     /db_xref="taxon:2737536"
                     /environmental_sample
                     /geo_loc_name="Canada"
                     /lat_lon="49.25 N 123.1 W"
                     /collection_date="2015-06-16"
                     /metagenome_source="wastewater metagenome"
                     /note="metagenomic"
     gene            complement(1..588)
                     /locus_tag="PLF56_01575"
                     /pseudo
     CDS             complement(1..588)
                     /locus_tag="PLF56_01575"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_002525803.1"
                     /note="incomplete; partial in the middle of a contig;
                     missing N-terminus and C-terminus; Derived by automated
                     computational analysis using gene prediction method:
                     Protein Homology."
                     /pseudo
                     /codon_start=1
                     /transl_table=11
                     /product="hypothetical protein"
     gene            complement(788..1492)
                     /locus_tag="PLF56_01580"
     CDS             complement(788..1492)
                     /locus_tag="PLF56_01580"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_020390090.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="metal-dependent transcriptional regulator"
                     /protein_id="HOQ52285.1"
                     /translation="MSDLIDTTEMYLRTIYELGEEGVPPLRARIAERLKQSGPTVSQT
                     VARMERDGLLTVDADRQLRLSPAGLKLATRVMRRHRLAERLLIDVIGLDWVHAHEEAC
                     RWEHVISTDVERRLVHLLHEPVESPYGNPIPALAELGAKPAPEAFLDGAMPLSEVLAQ
                     YPQALVTVRRLSEYLQTDRQLLAMFDASGVSPGAQLHATMDGHSLLLRADGRDDARFE
                     AQVADHLFVASDARAA"
     gene            1751..2434
                     /locus_tag="PLF56_01585"
     CDS             1751..2434
                     /locus_tag="PLF56_01585"
                     /inference="COORDINATES: protein motif:HMM:NF041024.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="acVLRF1 family peptidyl-tRNA hydrolase"
                     /protein_id="HOQ52286.1"
                     /translation="MLQHRLSIPPQRLPRWLDGFAQRHGSPTIAVEPGHLTLSAPNGA
                     EAEVELIWGPVGTGDPIGELLEQIARPRRLGALLIRKSSHAVGVFDGEALVASTIGRH
                     YVQGRTKAGGWSQQRYARRRENQAERAYAKAADAARTVLLPVVDELDGVILGGDARAL
                     REVLDDAGLAPLARLTKRLPKRTLAVPDPNLRVLRSALPQFVAVPISVNDVARFDSAP
                     AQHDDQAGH"
     gene            complement(2396..2710)
                     /locus_tag="PLF56_01590"
     CDS             complement(2396..2710)
                     /locus_tag="PLF56_01590"
                     /inference="COORDINATES: ab initio
                     prediction:GeneMarkS-2+"
                     /note="Derived by automated computational analysis using
                     gene prediction method: GeneMarkS-2+."
                     /codon_start=1
                     /transl_table=11
                     /product="hypothetical protein"
                     /protein_id="HOQ52287.1"
                     /translation="MAAAAKLGRVGAMIGALLVIMLVFTLPTTSRAALPRPEATTASA
                     TAKASPQPTASASETDVEPDDQPEDDGTDPTPDQSGTWIAIGGAAALSLVAGLIIVLR
                     RR"
     gene            3283..3936
                     /locus_tag="PLF56_01595"
     CDS             3283..3936
                     /locus_tag="PLF56_01595"
                     /inference="COORDINATES: ab initio
                     prediction:GeneMarkS-2+"
                     /note="Derived by automated computational analysis using
                     gene prediction method: GeneMarkS-2+."
                     /codon_start=1
                     /transl_table=11
                     /product="hypothetical protein"
                     /protein_id="HOQ52288.1"
                     /translation="MGELRLYAIGVDEVRDVFGAPPALERQFREIAAAAFVPQTTPKQ
                     PGMLGKLGPLFRRAPEAVIVQPDTPVEADVADLLAARYIRPERQPAAWRLLEAYLNAK
                     AWSTHRIELDVQRFNDLEFDLARAGMHPQFGLGRLVNNELSLPLQTYRGLATGCTKHS
                     QALQAASAWRNVLNHLSPENRSIAEPYAAWLAQFHHYAHAAPQQGRPLPDLVVVYRA"
     gene            complement(4222..5712)
                     /locus_tag="PLF56_01600"
     CDS             complement(4222..5712)
                     /locus_tag="PLF56_01600"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_014845832.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_component: GO:0005886 - plasma membrane [Evidence IEA];
                     GO_function: GO:0015205 - nucleobase transmembrane
                     transporter activity [Evidence IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="NCS2 family permease"
                     /protein_id="HOQ52289.1"
                     /translation="MVNSSPKAARQAAPAPSGMDSYFEITKRGSTIAHEVRGGLVTFF
                     TMAYIIVLNPLIIGTTPDKAGNLITGVPFATATDADKGGAIAAVAVATALIAGLTTIL
                     MGAYARFPIALATGLGLNAVVAYTIAPQMTWPQAMGLVVWEGIAITVLVLTGFRKAIF
                     NAVPRTLRSAISVGIGLFIAFIGLVDAGVVRTGSPIVQLGIFGSLRGWPIFLFVVVLL
                     LLVGLFVRKVKGAILIAILTGTVLAVIIESALNIGGKTDDNPTGWMLNVPSLGEFQTP
                     NFVLLGRVDLFGAFAGGPAVVLGLLLLIFSLLLSDFFDTMGTIVAVGAEGNLLQDDGN
                     PPRTQEILLVDSIAAIAGGVGSVSSNTAYIESAAGVGEGARTGLASLVTGAGFLLSIF
                     LAPLVDMVPSEAAAPALVFVGFLMMSQVVHVDWENPEQGIPAFFTMALMPFTYSITAG
                     IGAGFVSYAVIKLVLGKARTVHWLIYLVAALFVVYFAQGAILGLIS"
     gene            5916..6200
                     /locus_tag="PLF56_01605"
     CDS             5916..6200
                     /locus_tag="PLF56_01605"
                     /inference="COORDINATES: ab initio
                     prediction:GeneMarkS-2+"
                     /note="Derived by automated computational analysis using
                     gene prediction method: GeneMarkS-2+."
                     /codon_start=1
                     /transl_table=11
                     /product="hypothetical protein"
                     /protein_id="HOQ52290.1"
                     /translation="MSHDEAEHPLPTLQAPVKALDPHGLQIVGLGTVVFALGTLLCWW
                     QLPSLQSIGKGWWLSTALVGTGIGMLALIVLLIRRWRRLRNGPAVRRGTA"
     gene            complement(6339..7625)
                     /locus_tag="PLF56_01610"
     CDS             complement(6339..7625)
                     /locus_tag="PLF56_01610"
                     /EC_number="2.3.3.16"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_016454985.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_function: GO:0046912 - acyltransferase activity, acyl
                     groups converted into alkyl on transfer [Evidence IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="citrate synthase"
                     /protein_id="HOQ52291.1"
                     /translation="MSEVARLELDGTVYELPTFVGSEGERAIDISKLRDTTGFITLDD
                     GYGNTGSCRSAVTYIDGDAGILRYRGIPIEDLAGRSSFVETAWLVIFGTLPTVQQRDR
                     FSALLSDSAMIDEVMRKHFNAFPQSAPPMAILSAMINTLSAHDAGVTQIENDDDMERA
                     AAVLMSKVRTIAAASYKASIGEPLVYPRYDLKYAENFLHMMFSVPYRQFEPTPEVSAA
                     LNLFLMLHADHEQNCSTSTVRMVGSSKANLFASCAAGVCALWGPLHGGANVAAVQMLQ
                     QIHDSGMPVAEFVRKVKNKDDGVKLMGFGHRVYRNFDPRSKILKKSVDEVLAQLDKTD
                     PLLDIARELEAAALSDDYFIDRKLYPNIDFYSGILLRAIGIPLEMFTVMFAIGRMPGW
                     IANWKEINDEPKSRIYRPRQVYVGPTEVHWRPRDER"
     gene            complement(8446..9180)
                     /locus_tag="PLF56_01615"
                     /pseudo
     CDS             complement(8446..9180)
                     /locus_tag="PLF56_01615"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_004810791.1"
                     /note="incomplete; partial in the middle of a contig;
                     missing N-terminus; Derived by automated computational
                     analysis using gene prediction method: Protein Homology."
                     /pseudo
                     /codon_start=1
                     /transl_table=11
                     /product="DUF3027 domain-containing protein"
     gene            complement(9216..9602)
                     /locus_tag="PLF56_01620"
     CDS             complement(9216..9602)
                     /locus_tag="PLF56_01620"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_002532933.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_component: GO:0005829 - cytosol [Evidence IEA];
                     GO_function: GO:0001072 - transcription antitermination
                     factor activity, RNA binding [Evidence IEA];
                     GO_function: GO:0003676 - nucleic acid binding [Evidence
                     IEA];
                     GO_function: GO:0003723 - RNA binding [Evidence IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="cold-shock protein"
                     /protein_id="HOQ52292.1"
                     /translation="MPTGKVRYYDAEKGFGFLSKDDGGDDVFVRASALPDGVTSLKRG
                     QKVEFGVIDGKRGEQALSIHLLEAPPSLSKASRKSPDAMAVIVEDLIKMLDGLGNGYR
                     RGRYPDPKVSGKVAAVLRAVADELEL"
     gene            9740..10633
                     /locus_tag="PLF56_01625"
     CDS             9740..10633
                     /locus_tag="PLF56_01625"
                     /inference="COORDINATES: ab initio
                     prediction:GeneMarkS-2+"
                     /note="Derived by automated computational analysis using
                     gene prediction method: GeneMarkS-2+."
                     /codon_start=1
                     /transl_table=11
                     /product="hypothetical protein"
                     /protein_id="HOQ52293.1"
                     /translation="MANWTDGPEYAPLQRPAAFETPAARPLEVPPVVPNLADGAPPAQ
                     PEWQPPPTAAVPLDALVPASNLPARDPRAAFTTVSSLATTNSAWQSAHSTTGTLTQPA
                     WTPTQPLGTSAPPPTPAQGPAVAPVAGAWPAPSGAPVVAATPPRGLSFPPPAQAPSTF
                     PQPGTPDWFAPPTQGQWRPPDQSVTVAQMWQAATPGVMIPLIIGAFITPMSLVMLGAA
                     ALLSSRVRVRLAQVRRLFGYAFGLLAVIGVLSLFNSDFDLDSAWSVLSGWAQVLCWVV
                     SIVVLLQVGAGIRANEPPSRP"
     gene            10676..>10931
                     /locus_tag="PLF56_01630"
     CDS             10676..>10931
                     /locus_tag="PLF56_01630"
                     /inference="COORDINATES: ab initio
                     prediction:GeneMarkS-2+"
                     /note="Derived by automated computational analysis using
                     gene prediction method: GeneMarkS-2+."
                     /codon_start=1
                     /transl_table=11
                     /product="hypothetical protein"
                     /protein_id="HOQ52294.1"
                     /translation="MSDSPGLGRPSPGQPDFDGPPVDGTQPGSNLSGWTHVAGPRDDH
                     PATGEAQYGQSWHGQSWDGQSGQGQSWDGQSGQGQSWDGQS"
BASE COUNT         1796 a         3785 c         3679 g         1671 t
ORIGIN      
        1 gccgaagaag tgcccgaccg gggcgtgatg ccgcagtgcg accaccggtg agttgcggta
       61 cagcagagcg agccgcaccc gcgcgtccag gtcacgtcgc tgggcgaggg tgccgcgcac
      121 cgcccagcgg cacggcgtgg ggcaggtctc ggacacgtcc ggacgttcgc cggccgcgat
      181 cgtttcgtgg ttcatcgcgc acatggccag cgccgagccc tgacgggagt ggaaccggtc
      241 gaggtaggcg gtgaacccgg ccacgtcgcc gtccgcggca tgtaacgagc gcttggccgt
      301 ggcccgcatc gccgagtcgt gcgggcagct tcggtcgtcg gtcccccatc gctcccccac
      361 ccgctcggcc atccgcagcg gatcgccggc gtggccgagc gtcgactccc aggcgagttg
      421 gggcagatac tccccgatca gatgcgcggt cacgaccacc tgcagcggct cgtgccgcag
      481 ggcgcgcaac tcgagcacct cgagcatcag ccgatacagc gggaccagtg aggccagggc
      541 gccgcgttgc ggtgaacgcg gctccagcgg aaagctggcg ttgcccagtt cttcggccca
      601 atgccgtcgc gtgaactcgc gactgatcgt ccagtagtcc tcggcgtcga ggtcgaggac
      661 gcgcttggcg cagactgcca ggcgccaggc ttgttcgcgc gccggtgaac catggccggc
      721 ccggcgctcg gcatcgagca tcgcggccag cgcgtcatac cagtgccccc gcagcagggg
      781 cccggcatca agcggcacgg gcgtcgctgg ccacgaacag atggtcggcg acctgcgcct
      841 cgaaccgggc gtcgtcgcgc ccgtcggcgc gcagcagcaa cgaatgaccg tccatggtgg
      901 cgtgcagctg agcgccgggc gacaccccgg acgcatcgaa catggccagc agttgacgat
      961 cggtctgcag gtactcgctc agccttcgca cggtcaccag ggcctgcgga tactgcgcca
     1021 gcacctcgga cagcggcatt gcgccgtcga gaaacgcctc gggggctggc ttggcaccga
     1081 gctcggccag cgccgggatg ggattgccgt acggggattc gaccggctcg tgcaacagat
     1141 gcaccaggcg ccgctccacg tcggtcgaga tcacgtgctc ccagcggcac gcctcctcgt
     1201 gggcgtgcac ccaatcgaga ccgatcacgt cgatcagcag ccgttcggcg agccgatgcc
     1261 gtcgcatcac ccgggtcgcg agcttgagcc cggcgggact caaccgcaac tgacggtcgg
     1321 cgtcgacggt gagcaggccg tcacgttcca tgcgcgcgac ggtttgcgag acggtgggac
     1381 cgctctgctt gagccgttcg gcgatgcggg cacgcagggg cggcacaccc tcttcaccca
     1441 actcgtagat ggtgcgaagg tacatctcgg tcgtatcaat gaggtcgctc accggggccc
     1501 tccttcggtc gtgcagtcag attaggccat tggtaccacc cctgacgccg ccgcgtcgaa
     1561 accaccatcg ccgtggcggt cgcctcgagg tcaagggcct gtcgcagatg accctggccg
     1621 attcgcgagg accagcccgg tgcgtctgga cgacgagtcg atagcggatc ccgtccaagg
     1681 atgacagcgg gcggcgtggg tacgccgccg cgcggtcagg gtgacgcgac acgtcatcta
     1741 ggcttcgagc atgctgcagc accgcctgtc gattccgccg cagcgactgc cgcgctggct
     1801 ggacggtttc gcccagcgcc acggctcgcc caccatcgcg gtcgagcccg gccacctgac
     1861 cctgagcgct cccaacggcg ccgaggccga ggtcgaactc atctgggggc cggtgggcac
     1921 cggcgatccg atcggcgagt tactcgagca gatagcgcgc cctcgccggt tgggggcgct
     1981 gctgatccgc aagagttcgc acgccgtcgg cgtcttcgac ggcgaggcgc tcgtcgcgtc
     2041 cacgatcgga cgccactatg tgcagggccg caccaaggcc ggcggctggt cgcagcaacg
     2101 ctatgcccgg cgccgcgaga accaggccga gcgggcctac gccaaggctg ccgatgcggc
     2161 gcggacggtg ctgctgccgg tggtggacga actcgacggc gtgattctgg gcggcgatgc
     2221 gcgggccctg cgcgaggtgc tggacgatgc cggtctggcc cccctggccc ggctcacgaa
     2281 gcgattgccg aaaagaacgt tggccgtgcc tgacccgaac cttcgggtgc tgcggtcggc
     2341 cctgccacaa ttcgtggcgg tgccgatcag cgtcaacgac gtggcgcggt tcgactcagc
     2401 gcctgcgcag cacgatgatc aggccggcca ctagcgacag cgcggcagcc ccgccgatgg
     2461 cgatccacgt tccgctctga tcgggggtcg ggtcggtgcc gtcgtcctct ggttgatcgt
     2521 cgggttcgac atcggtttcg gacgcgctgg cggtcggctg cggcgacgcc ttcgcggtcg
     2581 ctgaagcggt cgtggcttcg ggtctcggca atgccgcgcg cgaggtggtg ggcagagtga
     2641 acaccagcat gatgacgagt agggcaccga tcatggcccc cacccggccc agttttgctg
     2701 ctgcggccat cacgctcctt ccggcgatgc gacgcacagc ctagcccgac ccgccatgac
     2761 tgccgccggg acgcgcccat gaggagcgtc cgcggcccgg gcgaagcgac ccgcgcgcca
     2821 ctcgccgccg ggtcaacgaa cgaagaaggc cccgatcagt gcgatcaaca ccagggcgat
     2881 cagcactccc gagacgatca ggcgacgcga ccgagggttc acccgtccaa tctatgtctg
     2941 ttgagtgtga ccgggccccc atcgcccgca agcgccctgg tgctgccacg tcaggggatc
     3001 cacggctcgc ccaggaacca tgtcccggct accgcacacc ccggacgggc gatccgaaga
     3061 ccccgtatct gggccgggat gaggggtggt tgtcctcacc ccctcaccct ggacgttccg
     3121 cgaagacctg cccggtgcgg ttgatcgcaa ggcggaagac gagtcgaaag cggggcccat
     3181 cgccggctgc caacgcagcg agcaggcgca ctggggggcc gcagcgcggc cagggtcagc
     3241 aggggttagc acgcacgccc tagtggcagg tacgttgtgc acatgggcga gttgcggctc
     3301 tacgcgatcg gtgtcgatga ggtgcgcgac gtgttcggcg ctccgccggc gctcgagcga
     3361 cagtttcgcg agatcgccgc ggccgccttt gtgccgcaga ccacacccaa gcagccgggc
     3421 atgctgggca agctcggccc cctgttccgg cgtgcgcccg aggccgtcat cgtgcagccc
     3481 gataccccgg tcgaggccga tgtcgccgac ttgctggccg cacggtacat acgtcctgag
     3541 cgccagcccg cagcgtggcg actactggag gcgtacctga acgccaaagc ctggagcacg
     3601 caccgtatcg agctggacgt gcagcgcttc aacgacctgg agttcgacct ggccagggcc
     3661 ggcatgcatc cccagttcgg cttgggcagg ctggtcaaca acgagttgag cctgccgttg
     3721 cagacctatc gcgggctcgc caccgggtgc accaagcaca gccaggcatt gcaggcggca
     3781 tccgcctggc gcaacgtgct gaaccacctc tcgcccgaga accgcagcat cgccgaaccc
     3841 tatgccgcct ggctggcgca gtttcaccac tacgcacacg cggccccgca acagggccgc
     3901 ccattgcccg atctggtcgt cgtgtaccgc gcctgacgac ccgtccagga tccgaaaccg
     3961 ctggtcgagt ccatcgaaac cgcgaacgag gggccccgac gagctcgatc aacggatctg
     4021 gacgagcctc tccaccagtg atcgcgccgg tcagaacagt gatggaggcg ttccgacgag
     4081 ctcagcaaac ggatcaggac gagcctgtcc accagtgatc gagccggtca cgaccgcgaa
     4141 cgaggggttc aacacgccca gccaatggat ctggacgagc ctgtccgact tctcgacaag
     4201 ctcgaccagc cgggtgtccg gtcagctgat caggcccaga atggcaccct gcgcgaagta
     4261 gaccacgaac agtgcggcga ccaggtaaat caaccagtgc accgtacgag ccttgccgag
     4321 caccagcttg atcaccgcgt acgaaacgaa acccgcgccg atgccggcgg tgatcgagta
     4381 cgtgaacggc atcagtgcca tggtgaagaa cgccggaatg ccctgctcgg ggttctccca
     4441 gtcgacgtgc accacctgcg acatcatcag gaagccgacg aacaccagcg ccggtgcggc
     4501 cgcttcggaa ggcaccatgt cgaccagtgg tgccagaaag atgctgagca ggaacccggc
     4561 gccggtgacc agcgacgcga ggcccgtccg agcgccctca cccacgccgg cggccgactc
     4621 gatgtaggcg gtgttcgagg acaccgagcc cacgccgccg gcgatggccg cgatcgagtc
     4681 caccagcagg atctcctggg tgcggggcgg gttgccgtcg tcctgcagca ggttgccctc
     4741 ggcgccgacg gcgacgatcg tgcccatggt gtcgaagaag tcgctcagca gcagcgagaa
     4801 gatcagcagc agcaggccca gcacgaccgc ggggccgccc gcgaaggccc cgaacaggtc
     4861 cacccgtccg aggagtacga agttgggcgt ttgaaactcg ccgagcgagg gcacgttcag
     4921 catccagccg gtcggattgt cgtcggtctt gccaccgatg ttcagcgcgg actcgatgat
     4981 cactgcgagc accgtgccgg tcagaatggc gatcagaatc gcgcccttga ccttgcggac
     5041 gaacaggccg accagcagca gcaacacgac gacgaacaag aagatcggcc agccccgcag
     5101 cgagccgaag atgcccaact gcacgatcgg gctaccggtg cgcacaacgc ccgcgtccac
     5161 caatccgatg aacgcgatga acaggccgat gccaaccgag atcgccgaac gcagcgtgcg
     5221 aggcacggcg ttgaagatgg ctttgcggaa cccggtgagc accagcacgg tgatcgcgat
     5281 tccctcccag accaccagac ccatggcctg cggccaggtc atctgcggcg cgatggtgta
     5341 cgccacgacg gcgttcaggc ccagtccggt cgccagggcg atcggaaacc gtgcgtaggc
     5401 acccatcaga atcgtggtga ggccggcgat cagtgccgtg gcgacggcga ccgccgcgat
     5461 ggcgccgccc ttgtcggcgt cggttgcggt ggcgaacggc acacccgtga tcaggttgcc
     5521 cgccttgtct ggtgtggtgc cgatgatcaa cgggttgagc acgatgatgt aggccatcgt
     5581 gaagaacgtg accaggccac cacgcacctc gtgcgctatg gtcgagcccc gcttggtgat
     5641 ctcgaagtat gagtccatgc cggacggggc cggggcggcc tggcgggccg ccttcggaga
     5701 tgagttgacc atggcgcaca ggctattcgc gcggcaggct caccttcggc gtgtctcgcc
     5761 acccggtggc gtcacatgag acgcccccaa cgggtggtcg cccggcggac gacccggccg
     5821 atgctctgcg tctcggggag ccggacgacg ccagtgaggg gtgcaacccc agccggtgcc
     5881 ggggtacggt acgcgtccgg cctagagtga ggtccgtgag ccacgatgaa gccgaacacc
     5941 ccctgccgac cctgcaggca ccggtcaagg ccctggatcc gcacggcctg cagatcgtcg
     6001 gtctcggcac cgtggtgttc gcgctcggca cgctgctctg ctggtggcaa ctgccgtcgc
     6061 tgcagagcat cggcaagggc tggtggttgt cgacggcact ggtcggcacc ggtatcggca
     6121 tgctcgcgct gatcgtgcta ctgatccgcc gctggcgacg cctgcggaac ggtcccgccg
     6181 tccgccgcgg gacggcctga accgacgccc gcccgagtct gtgcgcagat gcccgaccga
     6241 cgaaaggtcg gactgaccgc cacgccgttc gccgcgggac ggcttggacc gacgtccgcc
     6301 caagccgggc gcagatggtc gaccgacgaa ggtcggactt accgctcgtc cctcggacgc
     6361 cagtgcacct cggtgggtcc gacgtagacc tggcgcggcc gatagatgcg cgacttgggc
     6421 tcgtcgttga tctctttcca gttggcgatc cagccaggca tacggccgat cgcgaacatc
     6481 accgtgaaca tctccagcgg aatgccgatg gcccgcagca ggatgcccga gtagaaatcg
     6541 atgttcgggt acaacttgcg gtcgatgaag tagtcgtcgg acagcgccgc cgcttcgagc
     6601 tcgcgggcga tgtcgagcaa cgggtcggtc ttgtcgagct gggccagcac ctcgtccacc
     6661 gacttcttca ggatcttgct gcgtgggtcg aagttcctgt aaacgcggtg cccgaaaccc
     6721 atcaatttca cgccgtcgtc cttgttcttc accttgcgga cgaactctgc caccggcatg
     6781 ccggagtcgt ggatctgttg cagcatctgc accgctgcca cgttcgcgcc gccgtgcagt
     6841 ggcccccaca aggcgcagac gccggccgca caactcgcga acaggttcgc cttggacgaa
     6901 cccaccatgc gcacggtcga tgtggagcag ttctgttcgt gatcggcgtg cagcatcagg
     6961 aaaaggttga gcgcggccga cacctcggga gtcggctcga actggcggta gggcaccgag
     7021 aacatcatgt gcaggaagtt ctcggcgtac ttgaggtcgt agcgcgggta caccagcggc
     7081 tcaccgatgg acgccttgta cgacgcggcc gcgatggtgc gcaccttcga catgagcaca
     7141 gcggcggcac gttccatgtc gtcgtcgttc tcgatctggg tcacgccggc gtcatgcgcg
     7201 ctgagggtgt tgatcatcgc cgacagaatc gccatcggcg gcgcggactg cggaaaagca
     7261 ttgaagtgct tgcgcatgac ctcatcgatc atggccgaat ccgacagcag tgccgagaat
     7321 cggtcgcgct gctgcaccgt gggcagcgtg ccgaagatca ccagccacgc ggtctcgacg
     7381 aacgatgaac ggccggccag atcttcgatc ggaatgcctc ggtagcgcag aatgccggcg
     7441 tcgccgtcga tataggtgac cgccgagcga caggaaccgg tgttgccgta cccgtcgtcg
     7501 agggtgatga aaccggtggt gtcgcgcagc ttggagatgt cgatcgcccg ttcaccctcg
     7561 ctgccgacga acgtgggcaa ctcgtacacg gtgccgtcga gttcaagccg ggccacttca
     7621 gacatctggt ttctcctcgt cgtcgagttg gtgccgctgg gacacgataa cgggtgggcc
     7681 gcccggcgcg tcgagtgggt gaaggtgccg acattgtggc cgccggggtg tgctcgtcaa
     7741 cggtggtcga tgtggaggcc tgaaaaccct gcaccagtga cggggcgccc gcctcgccga
     7801 ccgacgaccc ttcgcactgg tgcagggttt tcaggccgac tcacgaggca ctgcagccgg
     7861 gacacgatgg cctggctgcg ggtctgattg tgagcgcgtg tgctgggtgc accgcagccc
     7921 gcgccggtga caagacgatg cgcggcgtac tcgtcatctc gggcgcggcg gcttcgtcat
     7981 cccgggcgcg gcgcacgcgt catcccgggc gcggctgttt cgtcatcccg ggcgcgtcgc
     8041 acgcgtcatc ccgggcttgc cccgggatct gaacgcacgc gtcatctcgg gcgcggcggt
     8101 ttcgtcatcc cgggcgcggc gcacgcgtca tcccgggctt gccccgggat ctcaacgcac
     8161 gcgtcatctc gggcgcggcg gtttcgtcat cccgggcgcg gcgcacgcgt catcccgggc
     8221 ttgccccggg atctcaacgc acgcagagat cccggcctgc gccggcatca cagttcgccc
     8281 acatcttcac cggctgcggc ttctccaaga tcccggcatg cgccgcgatg ccgagaatca
     8341 ccacgtcgag ggaacgctga tctgtgctcg atccgttgat tgaacgtcga aacccctggc
     8401 cgcacgggtc tcgaccagcg cgaccagcaa tcgatcagcg cttctctaga acaggccgag
     8461 gtcgaccgag accgtgtccc agatgggcgt ggccgcctgc tcgccgcggg agggctcggt
     8521 cacgtccgaa tgcgccccac agccgtgatc gcggctgacc acgtgaccat cgctcggcgt
     8581 gtactcgttg gtgcagacgc cgcagacgcg gcccatcgat cccgacaggg tgacgaagta
     8641 ggcgcaggtc tcgcacagcg caggcgcctg ggcggtcatc ggattgtcgg gaccgcccgg
     8701 tcccgcgatc cagcgctcga cggcttcatc gcgtccgaac ggcgagagca cccgctcgcg
     8761 accgaggccc agttcggcga ccaccgcgcg ggtggccgcc cactcggccg gctcggtgtt
     8821 gggcggcacc tcggtgacca cgaagcccgg ctcgacccgc ggatcgttgt cgggggtggg
     8881 catcagcacg ccgggcgaca ggtcaccggg accgatgcgt tcggcccacg gcacccagcg
     8941 cggcgccagc agcgactgcg gcccaggcat cagcgtgacc tcactgaccg tgaccacccg
     9001 cgccctggac gcccgcgcca ccgtcacctg ccacgaccag cccggatacc ccgggtgggg
     9061 gcagtcgaac aggtgcgtcg ccacccggac gtcatcgacc accacaccct ggtgctcgcc
     9121 gacccccagt accccggcgc gtgccactgc cgcggcgtgc gcgagttcga ccgcctcggc
     9181 acaggcgcga tcgaccgctt cggccaccgc catcgtcaga gctccagttc gtcggcgacg
     9241 gcgcgcagca cggctgccac cttgccggac accttcgggt cggggtagcg cccccggcgg
     9301 tagccgttgc cgaggccgtc cagcatcttg atcaggtctt cgacgatgac agccatcgcg
     9361 tccggcgact tgcgggacgc cttcgacaac gacggcggcg cctccagcag atggatgctg
     9421 agcgcctgct cgccgcgctt gccgtcgatc acaccgaact cgaccttctg gccgcgcttg
     9481 aggctggtca cgccgtcggg caatgccgag gcgcggacga acacgtcgtc gccgccgtcg
     9541 tccttggaga ggaagccgaa gcccttctcc gcgtcgtagt accgcacctt gccagtaggc
     9601 atgaccttct cctgttcgac caactcgcgg acacgcctgc agacggcgtc gtaggaccgc
     9661 acaagccagc gtatcgaagc aatcgcgccg gggcatccga agcgggttcc gctcgggggc
     9721 ccgtgactac tatgacgtca tggcgaactg gacggacggg cccgagtacg cgcccctgca
     9781 acgtccggcc gctttcgaga caccggcggc gcgtccgctg gaggtgcccc ccgtggtgcc
     9841 caatctggcc gacggagcgc cgccggcgca gcccgagtgg cagccaccgc cgacagccgc
     9901 agtgccattg gacgccctgg tgcccgcctc gaacctgccc gcacgcgacc cacgggccgc
     9961 cttcaccacg gtcagttcgt tggccacgac caactcggcc tggcagtcgg cccactccac
    10021 caccggcacc ctcacccagc ccgcctggac gcccacgcag ccgctcggca cgtcggctcc
    10081 gccgcctact cccgcccaag ggcctgccgt cgcaccggtg gcgggcgcgt ggccggcacc
    10141 gtccggggcg cccgtcgtcg cggccacacc tccgcgcggt ctcagctttc cgccgccggc
    10201 acaggctccc tcgacgtttc cgcaaccggg cacgcccgat tggttcgccc ccccgacgca
    10261 ggggcaatgg cggccaccag atcaaagcgt cacggtggcc cagatgtggc aggccgccac
    10321 accgggggtg atgattccgc tcatcatcgg cgcgttcatc acgccgatgt cgctggtgat
    10381 gctgggggcc gccgcgttgc tgtcgtcgag ggtgcgcgtt cggctcgcgc aggtgcggcg
    10441 actgttcggc tatgcgttcg ggttgctggc cgtgatcggg gtgctgagcc tgttcaactc
    10501 cgacttcgac cttgattccg catggtcggt gctctcgggc tgggcccagg tgctgtgctg
    10561 ggtcgtctcg atcgtggtgc tcctgcaggt cggtgcaggc atccgggcca acgaaccccc
    10621 gagccggccc tagtgtcgtg accccagcgg tctcccccag cgagaagagt caaccgtgag
    10681 cgattctccc ggcctcggcc ggcccagccc cggccagccc gatttcgacg gtccgccggt
    10741 tgacggcacg cagccggggt cgaacctgag cggctggacc cacgtcgccg gcccacgcga
    10801 cgatcatccg gcaacgggcg aggcacagta cggacagtcg tggcacggcc agtcgtggga
    10861 cgggcaatcc gggcagggcc agtcgtggga cgggcaatcc gggcagggcc agtcgtggga
    10921 cgggcaatcc g
//
LOCUS       DAOSUG010000025        10865 bp    DNA     linear   ENV 10-JUN-2024
DEFINITION  MAG TPA_asm: Micropruina sp. isolate AMR_MDS_2195
            NODE_3384_length_10865_cov_4.445513, whole genome shotgun sequence.
ACCESSION   DAOSUG010000025 DAOSUG010000000
VERSION     DAOSUG010000025.1
DBLINK      BioProject: PRJNA925477
            BioSample: SAMN32864262
            Sequence Read Archive: SRR4236673
KEYWORDS    WGS; ENV; Metagenome Assembled Genome; MAG; Third Party Data; TPA;
            TPA:assembly.
SOURCE      Micropruina sp. (wastewater metagenome)
  ORGANISM  Micropruina sp.
            Bacteria; Bacillati; Actinomycetota; Actinomycetes;
            Propionibacteriales; Nocardioidaceae; Micropruina.
REFERENCE   1  (bases 1 to 10865)
  AUTHORS   Abdulkadir,N., Saraiva,J.P., Zhang,J., Stolte,S., Gillor,O.,
            Harms,H. and Rocha,U.
  TITLE     Genome-centric analyses of 165 metagenomes show that mobile genetic
            elements are crucial for the transmission of antimicrobial
            resistance genes to pathogens in activated sludge and wastewater
  JOURNAL   Microbiol Spectr 12 (3), e0291823 (2024)
   PUBMED   38289113
REFERENCE   2  (bases 1 to 10865)
  AUTHORS   Abdulkadir,N.
  TITLE     Direct Submission
  JOURNAL   Submitted (24-MAR-2023) Environmental Microbiology, Helmholtz
            Center for Environmental Research -UFZ, Permoserstr 15, Leipzig
            04318, Germany
COMMENT     The annotation was added by the NCBI Prokaryotic Genome Annotation
            Pipeline (PGAP). Information about PGAP can be found here:
            https://www.ncbi.nlm.nih.gov/genome/annotation_prok/
            
            ##Genome-Assembly-Data-START##
            Assembly Method        :: SPAdes v. 3.11.1
            Genome Representation  :: Full
            Expected Final Version :: Yes
            Genome Coverage        :: Not Applicable
            Sequencing Technology  :: Illumina MiSeq
            ##Genome-Assembly-Data-END##
            
            ##Genome-Annotation-Data-START##
            Annotation Provider               :: NCBI
            Annotation Date                   :: 05/04/2023 04:57:10
            Annotation Pipeline               :: NCBI Prokaryotic Genome
                                                 Annotation Pipeline (PGAP)
            Annotation Method                 :: Best-placed reference protein
                                                 set; GeneMarkS-2+
            Annotation Software revision      :: 6.5
            Features Annotated                :: Gene; CDS; rRNA; tRNA; ncRNA
            Genes (total)                     :: 2,776
            CDSs (total)                      :: 2,750
            Genes (coding)                    :: 2,714
            CDSs (with protein)               :: 2,714
            Genes (RNA)                       :: 26
            tRNAs                             :: 24
            ncRNAs                            :: 2
            Pseudo Genes (total)              :: 36
            CDSs (without protein)            :: 36
            Pseudo Genes (ambiguous residues) :: 0 of 36
            Pseudo Genes (frameshifted)       :: 2 of 36
            Pseudo Genes (incomplete)         :: 32 of 36
            Pseudo Genes (internal stop)      :: 2 of 36
            ##Genome-Annotation-Data-END##
FEATURES             Location/Qualifiers
     source          1..10865
                     /organism="Micropruina sp."
                     /mol_type="genomic DNA"
                     /submitter_seqid="NODE_3384_length_10865_cov_4.445513"
                     /isolate="AMR_MDS_2195"
                     /isolation_source="Activated sludge of municipal
                     wastewater treatment plants"
                     /db_xref="taxon:2737536"
                     /environmental_sample
                     /geo_loc_name="Canada"
                     /lat_lon="49.25 N 123.1 W"
                     /collection_date="2015-06-16"
                     /metagenome_source="wastewater metagenome"
                     /note="metagenomic"
     gene            253..1371
                     /locus_tag="PLF56_01635"
     CDS             253..1371
                     /locus_tag="PLF56_01635"
                     /inference="COORDINATES: protein motif:HMM:NF017475.2"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="DUF808 domain-containing protein"
                     /protein_id="HOQ52295.1"
                     /translation="MSGFFALLDDIVAMAKLAAASLDDVGAAAGRASAKAAGVVIDDT
                     AVTPRYVHGFSPDREIPVVKRIAVGSLRNKLLFILPAALLLSQFLPWLLTPILMVGGT
                     YLCYEGAEKVWERFGGHAHAHTPTPVVGGPDEKAMVSGAIRTDFILSAEIMVIALNEV
                     ASEPLLSRTVTLIVVAVAMTLLVYGVVAVIVKMDDVGLHLATERSGWLAAFGRGLVAA
                     MPKVLATLSNVGIVAMLWVGGHILLVGVDELGWHPLYDLVHHAEESITHAVPGFAGET
                     LAWLVNTGVSAVIGLVVGAVVVAVLTLIRRFSTDANAPAAHAVAHEAADEGASESRAA
                     HDSADSAATGSAAEIESAGTGTTTRKPESSGTGAADPE"
     gene            1663..2217
                     /locus_tag="PLF56_01640"
     CDS             1663..2217
                     /locus_tag="PLF56_01640"
                     /inference="COORDINATES: protein motif:HMM:NF016840.2"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="DinB family protein"
                     /protein_id="HOQ52296.1"
                     /translation="MNENQSGASVRKPSAVDVLIESFGRIPGLLHDVVDDLDPDLLTV
                     QPRLTGGHAGNSIGWIVWHVGRMEDAQVAGIAGSAEVHDSGGWQQRLGTPYAARTHGY
                     GMSAADVARFSVSSASALTDYYEAVHTRTVEVLTSLDDQQLAAVVDERWTPPVTALVR
                     LVSIVDDAAQHAGQAAYVKGLLGG"
     gene            complement(2508..3755)
                     /locus_tag="PLF56_01645"
     CDS             complement(2508..3755)
                     /locus_tag="PLF56_01645"
                     /inference="COORDINATES: ab initio
                     prediction:GeneMarkS-2+"
                     /note="Derived by automated computational analysis using
                     gene prediction method: GeneMarkS-2+."
                     /codon_start=1
                     /transl_table=11
                     /product="hypothetical protein"
                     /protein_id="HOQ52297.1"
                     /translation="MRAYREILTLPGAWQFSAAGLLARSGGAMMGLGMVLMVSSLYGS
                     YGLAGALAASNAVAWALGTATLSNLVDRYGQRRVMYPAVIVSASALALLVVFAMLQLP
                     AWTLFPPAIVSGATGGAPGALVRARWNHATDDPDRLHTAYALESTLDELTFVIGPVLA
                     TALSTGVHPAAGLIAPVVLALVGAHLLYSQHSTEPPVLPRTYQGAGRPPLRSRLVLLM
                     PGVAAVVAVNMLIGCVFGAIDVSVVAAASEWDMRASSGVVLALFSLASGLAGFYYGSR
                     SWGSPLVARFLWGVSAMCAACLTLLLADSVLMLCLVGLVVGLTVAPTLINGNSLIGRL
                     VARERLTEGLAWMGTGIGIGVAIGSSLSGQVIDVAGYHGGFVTVAGFAALAAIIALAG
                     ARGLGHALARSSIDADEPEVAAS"
     gene            complement(3793..4434)
                     /locus_tag="PLF56_01650"
     CDS             complement(3793..4434)
                     /locus_tag="PLF56_01650"
                     /inference="COORDINATES: protein motif:HMM:NF024109.2"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_function: GO:0016787 - hydrolase activity [Evidence
                     IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="alpha/beta hydrolase"
                     /protein_id="HOQ52298.1"
                     /translation="MPVALVIFLHGLGHTPQMWQEQVTALPTGTKAVAPWLDGLRPGP
                     VVDFDLTRAADSVLGQLNRFGVDQAALVGTGLGSAVAVAAAGRSPQAVSHLVLADPLP
                     RVGRMAGGLQRMAVRAMPRAALAEAGLDRARMLTLMKTAAGLDLRRWLPQVEAPALVV
                     AGSGNPPGVTAGRDLAGAIAGARFELIGRAGACAPLDAPAEFNSLLYDFLAAG"
     gene            4635..5120
                     /locus_tag="PLF56_01655"
     CDS             4635..5120
                     /locus_tag="PLF56_01655"
                     /inference="COORDINATES: protein motif:HMM:NF015648.2"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_function: GO:0005515 - protein binding [Evidence IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="PH domain-containing protein"
                     /protein_id="HOQ52299.1"
                     /translation="MGLPAKLLAQGETELLHLRSHGKVMIKPTSWLLLIAALAGALFA
                     VLPASWRPWSGWFAVVAALGFAIPVYVVPLLRWRTTTSTLTNHRLITRTGIINKFGHD
                     VPVARITNVAYERSLTDRLFGCGSLVFTTSAEAPVVLHDIPRVEEVGVAISNLLFGAR
                     V"
     gene            5117..5551
                     /locus_tag="PLF56_01660"
     CDS             5117..5551
                     /locus_tag="PLF56_01660"
                     /inference="COORDINATES: protein motif:HMM:NF016060.2"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_component: GO:0005886 - plasma membrane [Evidence IEA];
                     GO_component: GO:0016020 - membrane [Evidence IEA];
                     GO_process: GO:0000271 - polysaccharide biosynthetic
                     process [Evidence IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="GtrA family protein"
                     /protein_id="HOQ52300.1"
                     /translation="MTTAPAARPALRTQLVRFIITGVLSAIVDYGLLVLLMAVGLGYV
                     PAKVLSFIAGTTTAYLINRRWTFVSDGSRRKFAAVMGLYALTFVLQVGIFTVLYPWLI
                     SVGADAEFMPKMNWAQLIGFVVAQGTATAVNFVVQRMVIFKP"
     gene            complement(5734..6360)
                     /locus_tag="PLF56_01665"
                     /pseudo
     CDS             complement(5734..6360)
                     /locus_tag="PLF56_01665"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_002517608.1"
                     /note="incomplete; partial in the middle of a contig;
                     missing C-terminus; Derived by automated computational
                     analysis using gene prediction method: Protein Homology.
                     GO_function: GO:0008233 - peptidase activity [Evidence
                     IEA];
                     GO_function: GO:0008236 - serine-type peptidase activity
                     [Evidence IEA];
                     GO_function: GO:0016805 - dipeptidase activity [Evidence
                     IEA];
                     GO_process: GO:0006508 - proteolysis [Evidence IEA]"
                     /pseudo
                     /codon_start=1
                     /transl_table=11
                     /product="peptidase E"
     gene            6609..7751
                     /locus_tag="PLF56_01670"
     CDS             6609..7751
                     /locus_tag="PLF56_01670"
                     /EC_number="6.3.4.18"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_002531235.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_function: GO:0004638 - phosphoribosylaminoimidazole
                     carboxylase activity [Evidence IEA];
                     GO_function: GO:0005524 - ATP binding [Evidence IEA];
                     GO_function: GO:0046872 - metal ion binding [Evidence
                     IEA];
                     GO_process: GO:0006189 - 'de novo' IMP biosynthetic
                     process [Evidence IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="5-(carboxyamino)imidazole ribonucleotide
                     synthase"
                     /protein_id="HOQ52301.1"
                     /translation="MTVRTIGIIGGGQLARMMHAASIPLGVRIALLAEGPDVSAAQVV
                     ADVSVGDYTDPDTVYGFAERCDVLTFDHEHVPPAILRGLIARGKGVHPGPDALLHAQD
                     KVVMRERLTALGVPCPEFAVVDDARELIACGERLGWPVIAKTSRGGYDGKGVWKIDDP
                     QQAGEPFAALRPGVRILAEEFIDFDRELSVLVARRPSGEERVYPVTETVQTDGICTHT
                     LTPAPGLSERQRADLEGLALRIAAELDVTGILAVELMQRPDGSVVVNELAMRPHNTGH
                     WSIDGAQTSQFENHLRAVADLPLGPTAPRQPHAAMVNVLGGARDDLPAAAAEVLATDP
                     GARIQLYGKSVVPGRKVGHVTVVGDDLSEVRRRAQAAADQLMGVQQ"
     gene            7748..8242
                     /gene="purE"
                     /locus_tag="PLF56_01675"
     CDS             7748..8242
                     /gene="purE"
                     /locus_tag="PLF56_01675"
                     /EC_number="4.1.1.21"
                     /EC_number="5.4.99.18"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_007076906.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_component: GO:0009320 - phosphoribosylaminoimidazole
                     carboxylase complex [Evidence IEA];
                     GO_function: GO:0004638 - phosphoribosylaminoimidazole
                     carboxylase activity [Evidence IEA];
                     GO_process: GO:0009152 - purine ribonucleotide
                     biosynthetic process [Evidence IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="5-(carboxyamino)imidazole ribonucleotide mutase"
                     /protein_id="HOQ52302.1"
                     /translation="MSGEPLVGIVMGSDSDWPVMQAAAQACDEFGVPYEADVVSAHRM
                     PEDMVAWGRAAHTRGLKVVIAGAGGAAHLPGMLAALTPLPVIGVPVPLKYLDGMDSLL
                     SIVQMPAGVPVATVAVGNARNAGLLAVRILSVADEVLQQRMLDFQAELRAAAQAKGAA
                     VRGR"
     gene            8541..10025
                     /locus_tag="PLF56_01680"
     CDS             8541..10025
                     /locus_tag="PLF56_01680"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_012919213.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="LCP family protein"
                     /protein_id="HOQ52303.1"
                     /translation="MTAATSLPDAQSRSQGIALRRDLTMLGMTLVVPGSAQLVAGNER
                     LGRLAVRIWLTLIALAVVFVLLLVVPFTRAFALGLYANPISLEVLRWASLVLGLGWAA
                     LFVNAWRIANPSALSPAGKVIVPAVAFVLALAVVWGSWTLSNAFEAQSRLVGNVFGGG
                     GKAETSSGRYNVLLLGGDAGAGREGLRPDTMIVASIDASTGRTVLFSLPRNLQWAPFP
                     ESSPLHKLYPNGYWCKDQSCLLNAVYTLAMDHKNLFPGVKNPGVQATREVIGNILGLD
                     INYYALVDLKGFEALINAVGGITLDVARKVPIGGGTDLTTGRKYPITGYIGPGKNLHL
                     NGHDALWFARSREGSTDYERMARQKCVLNAMVKQLNPTTVLTKFQDIAKAGEEIVSTD
                     VPTSQLGTLLDLAAKGRSLPLSSVSFAPPLIKPAKPDFPRIRQIVADTIEASENADDG
                     KGSKAESTPAQSASGSGSASAKPSAATTKSGTQTSDLDAVCTVS"
     gene            complement(10113..>10865)
                     /locus_tag="PLF56_01685"
     CDS             complement(10113..>10865)
                     /locus_tag="PLF56_01685"
                     /inference="COORDINATES: protein motif:HMM:NF024748.2"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="serine hydrolase"
                     /protein_id="HOQ52304.1"
                     /translation="SPTGSVRDQLQAVLEVIAAGQDKLGVAVQDLRSGASWNFRGDWA
                     SQSASMAKPMIVSMALRKARTDKLQQPLPAEQDALAQKAIKNSDNDAADALWTWAGAR
                     PAYDALAKDLGLKATHSAPEKDFWSWTWTTPDDQLSFMHQLVKGSTKALTDSERDYLL
                     GLMGQVEADQTWGVGAPKSASVQVQLKNGWVQFQSTDNLWAVNSIGHVEGDGRNYLLT
                     IMTRDSTFDLGKAYCNAIGDWVFRVLGSGTLT"
BASE COUNT         1704 a         3775 c         3754 g         1632 t
ORIGIN      
        1 tgccgatcgt cgaactgtca gcctgatcgg cgactgtccc ggggttcgcg atcgccttgt
       61 cgaccgccct ctctgctcgg cgtccgccgg acggcgcgta cacactgatc gcagtccggc
      121 agccggggcg cccgggctgc gggatcccgt ccgggcctcg ttccgcagag ctcgctcggc
      181 gtcccagggc cggcggagcc gcggcaccag tagggtggcc cgaatcgaaa ctcgatcgag
      241 gggtgggtgg gcgtgagcgg cttcttcgca ttgctggacg acatcgtcgc gatggccaag
      301 ctcgcggccg cctcactgga cgatgtcggc gccgccgccg gacgtgccag cgccaaggcg
      361 gccggcgtgg tgatcgacga cacagccgtg acgccgcgtt acgtgcacgg gttcagccct
      421 gatcgcgaaa tacctgtggt caagcggatc gccgttgggt cgctgcgcaa caagctgctg
      481 ttcattctgc ccgcggcgct gctgctgagt cagtttctgc cctggctgtt gaccccgatc
      541 ctgatggtcg gcggtaccta cctgtgctac gagggcgcgg aaaaggtctg ggagcggttc
      601 ggcggtcacg cgcatgctca cacaccgacc cccgttgtcg gcggcccgga cgagaaggcg
      661 atggtcagcg gagcgatccg caccgacttc attttgtcgg ccgagatcat ggtgatcgcg
      721 ctcaacgagg tcgcctccga gcctctgctc agccgcacgg tcacgttgat cgtggtggcg
      781 gtggccatga cgctgctcgt gtacggcgtg gtggcggtga tcgtcaagat ggacgatgtc
      841 ggcctgcatc tggccaccga acggtccggc tggctggccg cattcggacg gggcctggtg
      901 gcggcgatgc cgaaggtgct ggcgacactg tcgaacgtgg gcatcgtggc catgctgtgg
      961 gtgggcggcc acattctgct ggtcggcgtc gacgaactgg gctggcatcc gctctacgac
     1021 ctggtgcatc acgccgaaga gtcgatcacg cacgctgtac cggggttcgc cggcgagaca
     1081 ctcgcctggc tggtgaacac cggcgtctcg gcggtgatcg gcttggtggt cggcgcggtg
     1141 gtggtggccg tgctcacgct gatccgccgg ttcagcactg acgcgaacgc acccgccgcc
     1201 cacgccgtgg cacacgaggc tgcggacgag ggggcatccg agagtcgggc cgcgcacgat
     1261 tcggctgatt ccgcggcgac cggatcggca gccgagatcg agtcggccgg taccgggacc
     1321 acgacgagga agcccgaatc gagcggtacc ggtgccgctg atcccgaata gagcggtgcc
     1381 ggcgcagccg aaccggggct gcttggtgcc ggggcgtcgg aacctgggtc ggacgacgct
     1441 gaggtgacga gccccgggtc gtccggggcc gggggactgg agcctaagtc ggacggcgag
     1501 gtgacgaaac ccgggtcgtc cggggccggg ggagtggagc ctgggttgga cgacgccgag
     1561 ctgacgaaac ccgggtcggc cggggcgtca gggtagaccc cgcaaccccg tcgttacggc
     1621 cgagccctga cttctgtcgg tgatcccgcg tagcgtgaca tcgtgaatga gaaccagagc
     1681 ggtgccagcg tgcgaaagcc cagcgccgtc gacgtgctga tcgaatcgtt cggccgcatt
     1741 cccggcctgc tgcacgatgt ggtcgatgac ctcgaccctg atctgctcac cgtgcagccc
     1801 cggctgaccg gcgggcacgc cggcaactcg atcggttgga tcgtctggca cgtcggccgc
     1861 atggaagatg cgcaggtcgc cggcatcgcc ggctcggccg aggttcacga cagcggtggt
     1921 tggcagcaac gcctcggcac gccctacgcg gcccgaacgc atggttacgg catgagcgcc
     1981 gccgatgtcg cccgcttctc ggtcagttct gcctctgcct tgaccgacta ctacgaggcg
     2041 gtgcacaccc gcactgtcga ggtgctgaca tcgttggatg atcagcaact cgcggccgtc
     2101 gtcgacgagc gctggacccc gcccgtgacc gcgctcgtcc ggctggtcag tatcgtcgac
     2161 gacgccgctc agcacgccgg gcaggcggcc tacgtcaagg gcctgttggg cggttgaccc
     2221 gctgagcccc gatcggtggg ttcggactca ctacgggtgg ggtcggactc actacgggtg
     2281 ggtttcggca ccgactgctg tagcaaaacc cacccgttcg agcacggctc accgaagccg
     2341 gctcatcgac cgtggccgtc ggccttcttg cgacgagtcc gggccgggcg atgacggcct
     2401 tcggtgtgga tccggcgata gaaaccgctg gtctactcgt cctcctgcct gcgcccagga
     2461 gctgacggcc ggcgtcaagc gccaccagcc gccgcccggc attcggctca gctcgcagcc
     2521 acctcgggct cgtccgcgtc gatgctgctg cgcgcgagcg cgtgcccaag gccgcgggcg
     2581 cccgccagcg caatgatggc ggccagggcg gcgaagccgg ccacggtgac gaagccgccg
     2641 tggtagccgg ccacatcgat cacctgaccc gacaggctgg agccgatcgc cacgccgatg
     2701 ccgatgccgg tccccatcca cgccagccct tcggtgaggc gttcacgggc gaccagtcgt
     2761 ccgatcaatg agttgccgtt gatcagcgtg ggagcgaccg tgaggcccac caccaggccg
     2821 accaggcaga gcatcagcac cgagtcggca agcagcaggg tcaggcaggc cgcgcacatg
     2881 gccgacactc cccacagaaa gcgcgcaacc aacggcgagc cccaggaacg cgagccgtag
     2941 tagaagccgg ccaggccgga cgccagcgag aacagcgcca gcaccacgcc ggacgacgcg
     3001 cgcatgtccc actcgctcgc ggccgccacc accgagacgt cgatcgctcc gaacacgcag
     3061 ccgatcagca tgttcaccgc caccacggca gcgacgcccg gcatcagcag caccagccgg
     3121 ctgcgcaacg ggggtcgtcc ggcgccctgg taggtgcggg gcagtaccgg cggctcggtg
     3181 ctgtgctggg agtacaacag gtgcgcgccg accagggcga gcaccacggg agcgatcagt
     3241 cccgccgccg gatggacgcc cgtcgagagc gccgtcgcga gaacgggtcc gatcacgaag
     3301 gtcagctcat ccagggtgga ttcgagggcg tatgcggtgt gcaggcggtc gggatcgtcc
     3361 gtggcgtggt tccagcgggc ccggacgagt gcgccgggcg cgccgccggt ggcgcccgag
     3421 acgatggccg gtgggaagag cgtccaagcc ggcagttgca gcatcgcgaa gaccaccagc
     3481 agcgcaagtg ccgaggccga cacgatcacc gcggggtaca tcacccggcg ttgcccgtag
     3541 cgatcgacga ggttcgacag cgtggccgta cccagggccc aggccaccgc attggacgcg
     3601 gccagcgctc cggccaggcc gtagctgcca tagagcgagg agaccatcag caccatgccg
     3661 aggcccatca tggctccgcc ggagcgagcc agcagccctg ccgcgctgaa ctgccaggcg
     3721 ccggggaggg tcaggatctc ccggtaggca cgcacctctc tattctggca caagcgtgcg
     3781 gaaacgggcc aatcaaccgg cggcgagaaa gtcgtacagc aagctgttga actcggcggg
     3841 cgcgtccagg ggtgcacagg cccccgcgcg cccgatcagc tcgaaacggg cgcctgcgat
     3901 ggcgccggcc aggtcgcgac cggccgtcac cccgggcggg ttgcccgagc cggccaccac
     3961 cagggcaggg gcctcgacct gcggcaacca gcgccgcagg tcgaggcccg ctgcggtctt
     4021 catcagcgtg agcatgcggg cccgatccag gcccgcctcg gccagcgcgg cgcggggcat
     4081 ggcacgcacc gccatgcgtt gtagcccgcc ggccattcgt ccgacccggg gcagggggtc
     4141 ggcgagcacc agatgcgaca ccgcctgcgg ggaccgtccg gccgccgcca ccgccaccgc
     4201 cgagccgaga ccggtgccga ccagggccgc ctggtcgacc ccgaaccggt tcagctggcc
     4261 gaggaccgag tcggctgcgc gggtcaggtc gaagtcgacc acggggcccg gacgcagccc
     4321 gtccagccag ggcgcgacgg ccttggtgcc ggtcggcagt gcggtcacct gctcctgcca
     4381 catctggggg gtgtggccga ggccgtgcag aaagatcacc agagcgacgg gcatgcgccc
     4441 atcgaaccat gctcggcccg cccaccgctg ctgtggaccc gagggcagcg gccgcgtttc
     4501 gacgttgcgc cggttggtgc gccgggttgc cgcgcagtgg gggtgcaccc cggccgcccg
     4561 caaccgatgc gtccacctgc gcgcgcagca ccgtcacgat cgcggcgggc ccggcggccg
     4621 acataatgga cggcatggga ctgcctgcga agctgctcgc acagggcgag accgaactgc
     4681 tgcatctgcg ctcgcacggc aaggtgatga tcaagccgac cagctggctg ctactgatcg
     4741 ccgcgctggc cggggcgctc ttcgccgtat tgccggcgtc gtggcgtccg tggtccgggt
     4801 ggttcgcggt cgtggcggcg ctggggtttg cgataccggt ctacgtggtg ccgctgctgc
     4861 gctggcgcac gactaccagc acgctcacca accaccggct gatcacccgc accggcatca
     4921 tcaacaagtt cggccacgac gtgcccgtcg cccgcatcac caacgtcgcc tatgagcgca
     4981 gcctgaccga tcggctgttc ggctgcggct cgctggtgtt caccacctcc gccgaggcgc
     5041 cggtggtgct gcacgacatt cccagggtcg aagaggtggg cgtcgccatc tcgaatctgc
     5101 tgttcggagc gcgagtatga ccacggcccc cgccgcacgg ccggcgctgc gcacccaact
     5161 cgtccgcttc atcatcaccg gcgtgctgtc cgcgatcgtc gactacgggc tgctggtgct
     5221 gctgatggca gtcggcctgg gctatgtgcc ggccaaggtt ctgtcattca tcgcaggcac
     5281 caccaccgcc tacctgatca accgccgctg gaccttcgtc tcggacggat cccgccgcaa
     5341 gttcgccgcc gtgatgggcc tctacgcgct gactttcgtg ttgcaggtcg gcatcttcac
     5401 cgtgctctac ccctggttga tcagtgtcgg tgcggacgcc gagttcatgc cgaagatgaa
     5461 ctgggcgcag ttgatcggct tcgtggtggc ccagggcacg gccacggcgg tcaacttcgt
     5521 cgtgcagcgc atggtgatct tcaagccctg accaagcacc gagcgccccc tcaactatct
     5581 ctggcctgcg aagcttcgca ggccagagat agttgagggg gcgctcagcg gtgggccggg
     5641 ggctcaggag ctgagcaggc tcatctgctg cggttcgcga atcaccgccg gcccgggcac
     5701 gtcgttgggc agcacccggt acaccacctg gccctcgcgt tcggccagaa agtgagccgg
     5761 tacgccgtcc acgtaatgca ccccggcgcc gtcgtcggcc gcgtagccgt ccggcagggc
     5821 gccggaggcg acggcggagg tgaagaccgg gtcgcgctcg ggttcgccgt ccagatgggg
     5881 gcagaaacta ccgcgcacca gcccgagacc gccgcgccag ggccgcagat cgccgaagct
     5941 gtcggtgacg cacccctgga accaacaggc cgcgcccgct gagatgccgg ccagcacgag
     6001 cgcgccgtcg gtgtagcgct ggcgcaccac ttcggtcacg ccatgggcct cccacaaggc
     6061 catcaggttg accgtgctac ccccgccgac gaacaagagg tcggcttcac ccagccgatc
     6121 cacggcctgt ccggcgcccg accacagcgt gagcaccgag gttcgcacgc cgagcgcccc
     6181 ataggcggtg agaaagcggt gaatgtaggc ggggtcgtcg cccgacgccg tcggggcgaa
     6241 gcagacctgc gggtgatcct tgccggtgag gcggacgaga tagcgatcga gcgccgtcgg
     6301 ttcaccgaac tgcgacatcg agaacccgcc accgcccatg gccacaatgt gccgtgtcat
     6361 ggccgtcatc ctagtgtcgg gtcgctctgg tggggggccg ttcggcgttg tcgagggtcg
     6421 tgcaccgcaa ggccgtccag acagcgggac gggtctttcg accgaacccg cccccgccag
     6481 gtgccccaac ccacccaccg agcgcccccc caactatctc tggcccgcga agcttcgcgg
     6541 gccagagata gttgaggggg cgctcggtga gcgatcgctc aggcgccgag caggctaggg
     6601 tttcgcgggt gaccgtgcga accatcggca tcatcggcgg cggccagttg gcgcgcatga
     6661 tgcatgccgc cagtattccg ctgggggtgc gcatcgccct gctcgccgag ggccccgacg
     6721 tctcggccgc ccaggtggtg gccgatgtca gcgtgggcga ctacaccgat cccgacaccg
     6781 tctacggctt cgccgaacgc tgcgacgtgc tcacgttcga ccacgagcac gtgccgcccg
     6841 cgatcctgag ggggctgatc gcgcgcggca agggcgtcca ccccggcccg gacgccctgc
     6901 tgcacgccca ggacaaggtg gtgatgcgtg agcggctcac cgcgctgggt gtgccgtgcc
     6961 ccgagttcgc agtggtggac gatgcccgcg aactgatcgc gtgcggcgag cggctcggct
     7021 ggccggtgat cgccaagacc tcccgcggtg ggtacgacgg caagggcgtg tggaagatcg
     7081 acgacccgca gcaggcaggc gagccgttcg ccgcgctgcg gcccggggtg cggatcctgg
     7141 ccgaggagtt catcgacttc gaccgcgagc tgagcgtgct ggtggcgcgg cgtccgagcg
     7201 gtgaggaacg ggtctacccg gtcaccgaga ccgtgcagac cgacggcatc tgcacccaca
     7261 ccctcacccc ggcccccggt ttgagcgagc ggcagcgggc cgacctcgaa ggcctcgcac
     7321 tgcggatcgc cgccgaactc gacgtcacgg gcatactcgc ggtcgaactc atgcaacgcc
     7381 cggacggcag cgtggtggtc aacgagttgg cgatgcgtcc gcacaacacc ggccactgga
     7441 gcatcgacgg cgcccagacc tcgcagttcg agaaccacct tcgtgccgtt gccgacctcc
     7501 ccctgggccc caccgctccg cggcagcccc acgcggcaat ggtcaacgtg ctcggcggtg
     7561 cccgcgacga cctgccggcc gccgccgccg aggtgctggc caccgacccg ggcgcgcgca
     7621 tccagttgta cggcaagagc gtggtgcccg gacgcaaggt cggccacgtc acggtggtcg
     7681 gcgacgatct gtccgaggta cggcggcgtg cgcaggcggc cgccgatcaa ctgatgggag
     7741 tgcagcaatg agcggggaac ccctggtcgg aatcgtgatg ggctccgact cggactggcc
     7801 ggtgatgcag gccgccgccc aggcctgcga cgagttcggc gtgccctatg aggcggacgt
     7861 ggtctcggcc caccgcatgc cggaagacat ggtcgcctgg ggacgggccg cgcacacccg
     7921 cgggctcaag gtggtgatcg ccggcgccgg gggagccgcg cacctgccgg gcatgctggc
     7981 cgcactcacc ccgctgccgg tgatcggagt gccggtgccg ttgaaatacc tcgacggcat
     8041 ggattcgctg ctgtcgatcg tgcagatgcc ggccggtgta ccggtggcga ccgttgcggt
     8101 cggaaacgcc cgcaacgctg gactgctggc cgtgcgcatc ctctcggtgg cggacgaggt
     8161 gctgcagcaa cgcatgctgg actttcaggc cgagctgcgg gcggcagcgc aggccaaggg
     8221 cgcggccgtc cgggggcgtt gacccggagc ggggtcggca agaactcggt gggtccggaa
     8281 gcgccgcggg cggatacccg aaaaacactc atccctcgct ggttgagctg gtcgggactc
     8341 catcgctggt tgagctggtc gggaccctac gaccagggga ctcggccagg ttcagccaac
     8401 gggtcgcgcc cttgatcagc acgtgcccga cccgccgagg ttagccccgc cgcggcatcg
     8461 tcccgccgtg ttgtcagaca cctgcctcac ccccggcgag ccgaccgaat gggcttgggg
     8521 acaggcggca gactggttcg gtgaccgccg ccacctccct gcccgacgcc cagagccgca
     8581 gccagggcat cgccctgcgc cgcgacctga ccatgctcgg catgacgctg gtcgtgcccg
     8641 gatcggccca gttggtcgcc ggtaacgagc ggctcggacg tctcgccgtt cggatctggc
     8701 tgaccctgat cgcgctggcc gtggttttcg tgctgctgct ggtggtgccc ttcacccggg
     8761 ccttcgccct cggcctctac gccaacccga tcagcctcga agtgctgcgc tgggccagcc
     8821 tggtgctggg tctgggctgg gccgcgctgt tcgtgaacgc ctggcgcatc gccaacccgt
     8881 ccgccctgtc accggccggc aaggtgatcg tgcccgcggt cgccttcgtg ctcgccctcg
     8941 ccgtggtttg gggttcgtgg acgctctcga acgccttcga ggcacagagc cggctggtcg
     9001 gcaacgtgtt cggcggcgga ggcaaggccg aaaccagctc cggtcgctac aacgtgctgc
     9061 tgctcggcgg cgacgccggc gccggacggg aggggctgcg tcccgacacc atgatcgtgg
     9121 ccagcatcga cgcctccacc ggacgcaccg tgctgttcag cctgccccgc aacctgcagt
     9181 gggcgccgtt cccggagtca tcgccgctgc acaagctgta cccgaacggc tactggtgca
     9241 aagaccagtc atgcctgctc aacgcggtgt acacgctggc gatggaccac aagaacctgt
     9301 ttccaggggt gaagaacccc ggcgtgcagg ccacccgaga ggtgatcggc aacattctcg
     9361 ggctcgacat caactactac gcgctggtcg acctcaaggg cttcgaggcg ctgatcaacg
     9421 ccgtgggcgg catcacgctc gacgtggccc gcaaggtgcc gatcggcggc gggaccgacc
     9481 tgaccacggg ccgcaagtac cccatcaccg gctacatcgg cccgggcaag aacctgcatc
     9541 tgaacggcca tgacgcgctg tggttcgccc gctcgcgcga aggctccacc gactatgagc
     9601 gcatggcgcg gcagaaatgc gtcctgaatg ccatggtcaa gcagctcaac ccgaccacgg
     9661 tgctgaccaa gttccaagac atcgccaagg cgggcgaaga gatcgtgtcg accgacgtgc
     9721 ccacctcgca actgggcaca ctgctcgact tggccgccaa gggccgctcg cttccgctca
     9781 gcagcgtcag cttcgccccg ccgctgatca agcccgccaa gcccgacttt ccccgcatcc
     9841 ggcagatcgt tgccgacacc atcgaggcgt ccgagaacgc cgatgacggc aagggcagca
     9901 aggccgagag caccccggcg cagtccgcca gcggtagcgg atcagcctcg gccaagccgt
     9961 ccgccgcgac caccaaatcg ggcacgcaga ccagcgatct ggacgccgtc tgcaccgtca
    10021 gctgacccgg catccggctc accggatcga ccgcgttcgg tgcgccgagc gagaacccga
    10081 cgttcgactc acagcccgga cgggggagga gctcaggtca gcgtgccgga gcccaacacg
    10141 cggaacaccc agtcgccgat cgcattgcag taggccttgc ccagatcgaa ggtggagtcg
    10201 cgggtcatga tggtcagcag gtagttgcgt ccgtcgccct cgacgtgacc gatcgagttg
    10261 accgcccaca ggttgtcggt cgactgaaac tgcacccagc cgttcttcag ttgcacctga
    10321 accgacgcgc tcttcggcgc accgaccccc caggtctggt cggcctcgac ctgccccatc
    10381 aggcccagca ggtagtcgcg ttcgctgtcg gtcaacgcct tcgtcgagcc cttcaccaac
    10441 tgatgcatga acgacaactg gtcatcgggc gtggtccagg tccagctcca gaagtccttc
    10501 tcgggtgcgc tgtgggtggc cttgaggccc aggtccttgg ccagcgcgtc gtaggccgga
    10561 cgggcgcccg cccaggtcca cagggcgtcc gcagcgtcgt tgtcggagtt cttgatcgcc
    10621 ttctgggcca gcgcgtcctg ctcggccggc agcggctgct gcagcttgtc ggtgcgcgcc
    10681 ttgcgcagcg ccatcgacac gatcatcggc ttggccatgg acgcactctg gctggcccag
    10741 tcgccgcgaa agttccagct ggcaccgctg cgcaggtcct ggacggcgac gcccagcttg
    10801 tcctggcccg ccgcgatcac ctccaacacc gcctgcagct gatcgcggac cgatccggtg
    10861 ggcga
//
LOCUS       DAOSUG010000026        10759 bp    DNA     linear   ENV 10-JUN-2024
DEFINITION  MAG TPA_asm: Micropruina sp. isolate AMR_MDS_2195
            NODE_3424_length_10759_cov_4.716087, whole genome shotgun sequence.
ACCESSION   DAOSUG010000026 DAOSUG010000000
VERSION     DAOSUG010000026.1
DBLINK      BioProject: PRJNA925477
            BioSample: SAMN32864262
            Sequence Read Archive: SRR4236673
KEYWORDS    WGS; ENV; Metagenome Assembled Genome; MAG; Third Party Data; TPA;
            TPA:assembly.
SOURCE      Micropruina sp. (wastewater metagenome)
  ORGANISM  Micropruina sp.
            Bacteria; Bacillati; Actinomycetota; Actinomycetes;
            Propionibacteriales; Nocardioidaceae; Micropruina.
REFERENCE   1  (bases 1 to 10759)
  AUTHORS   Abdulkadir,N., Saraiva,J.P., Zhang,J., Stolte,S., Gillor,O.,
            Harms,H. and Rocha,U.
  TITLE     Genome-centric analyses of 165 metagenomes show that mobile genetic
            elements are crucial for the transmission of antimicrobial
            resistance genes to pathogens in activated sludge and wastewater
  JOURNAL   Microbiol Spectr 12 (3), e0291823 (2024)
   PUBMED   38289113
REFERENCE   2  (bases 1 to 10759)
  AUTHORS   Abdulkadir,N.
  TITLE     Direct Submission
  JOURNAL   Submitted (24-MAR-2023) Environmental Microbiology, Helmholtz
            Center for Environmental Research -UFZ, Permoserstr 15, Leipzig
            04318, Germany
COMMENT     The annotation was added by the NCBI Prokaryotic Genome Annotation
            Pipeline (PGAP). Information about PGAP can be found here:
            https://www.ncbi.nlm.nih.gov/genome/annotation_prok/
            
            ##Genome-Assembly-Data-START##
            Assembly Method        :: SPAdes v. 3.11.1
            Genome Representation  :: Full
            Expected Final Version :: Yes
            Genome Coverage        :: Not Applicable
            Sequencing Technology  :: Illumina MiSeq
            ##Genome-Assembly-Data-END##
            
            ##Genome-Annotation-Data-START##
            Annotation Provider               :: NCBI
            Annotation Date                   :: 05/04/2023 04:57:10
            Annotation Pipeline               :: NCBI Prokaryotic Genome
                                                 Annotation Pipeline (PGAP)
            Annotation Method                 :: Best-placed reference protein
                                                 set; GeneMarkS-2+
            Annotation Software revision      :: 6.5
            Features Annotated                :: Gene; CDS; rRNA; tRNA; ncRNA
            Genes (total)                     :: 2,776
            CDSs (total)                      :: 2,750
            Genes (coding)                    :: 2,714
            CDSs (with protein)               :: 2,714
            Genes (RNA)                       :: 26
            tRNAs                             :: 24
            ncRNAs                            :: 2
            Pseudo Genes (total)              :: 36
            CDSs (without protein)            :: 36
            Pseudo Genes (ambiguous residues) :: 0 of 36
            Pseudo Genes (frameshifted)       :: 2 of 36
            Pseudo Genes (incomplete)         :: 32 of 36
            Pseudo Genes (internal stop)      :: 2 of 36
            ##Genome-Annotation-Data-END##
FEATURES             Location/Qualifiers
     source          1..10759
                     /organism="Micropruina sp."
                     /mol_type="genomic DNA"
                     /submitter_seqid="NODE_3424_length_10759_cov_4.716087"
                     /isolate="AMR_MDS_2195"
                     /isolation_source="Activated sludge of municipal
                     wastewater treatment plants"
                     /db_xref="taxon:2737536"
                     /environmental_sample
                     /geo_loc_name="Canada"
                     /lat_lon="49.25 N 123.1 W"
                     /collection_date="2015-06-16"
                     /metagenome_source="wastewater metagenome"
                     /note="metagenomic"
     gene            complement(<1..692)
                     /locus_tag="PLF56_01690"
     CDS             complement(<1..692)
                     /locus_tag="PLF56_01690"
                     /inference="COORDINATES: protein motif:HMM:NF012742.2"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_function: GO:0000976 - transcription cis-regulatory
                     region binding [Evidence IEA];
                     GO_function: GO:0003700 - DNA-binding transcription factor
                     activity [Evidence IEA];
                     GO_function: GO:0043565 - sequence-specific DNA binding
                     [Evidence IEA];
                     GO_process: GO:0006351 - DNA-templated transcription
                     [Evidence IEA];
                     GO_process: GO:0006355 - regulation of DNA-templated
                     transcription [Evidence IEA];
                     GO_process: GO:0045892 - negative regulation of
                     DNA-templated transcription [Evidence IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="LacI family DNA-binding transcriptional
                     regulator"
                     /protein_id="HOQ52305.1"
                     /translation="MPSTTRRGRRKTGPSIEDVARLAGVSAQTVSRVSTGAEAVRDET
                     KAKVLQAMDQLGYSPNRAARALRSGSFGTIGLIAHRFERTGEALTTEAVVEAAESEGL
                     SVTLMNVRTPAADQWEHAALRLSHQAIDGLVIIRAEQATPDSLALPAGMAVAVSDSRL
                     IGHYPSVVADQVQGSEAATRHLLGLGHRIVHHLAGPSDSEPALVRAATWQRCLDEAGV
                     LAPALVRGDWSAR"
     gene            921..2240
                     /locus_tag="PLF56_01695"
     CDS             921..2240
                     /locus_tag="PLF56_01695"
                     /inference="COORDINATES: protein motif:HMM:NF024808.2"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="extracellular solute-binding protein"
                     /protein_id="HOQ52306.1"
                     /translation="MNRFSKMAALAVTALLSVGMAACSGGATATSPAATSATSTGPKS
                     GKLTVWAWDPTFNIYAMKEAEKIYQKDNPGFSLEIVETPWDDLQTKLTTLAQSKETKQ
                     LPDIFLMQNNAFQKNVINYPDLFSDYAKSGVDFSQFPAGVTAYSTVDNKNYGLPFDNG
                     TAIQALRTDVIKEAGYTMADFTDITWSDFLTKAKAVKAKTGKALLSGQAGSSDLIMMM
                     LQSAGASLFDAEGKPTITNNPALLKSIDVYKQLVDAKVLTEVNSWDEYIGTFVNGTVA
                     GTINGVWIMGSIQTAKDQSGKWQITNLPKLDGVEGATNYSANGGSSWAISSNANYDLA
                     ADFLNKTFAGSKPLYETILPSSGAVANWLPMADSTVYSQPQKFFGDQPVFSKVIEFSK
                     QVPKNNTGAYYYEGRDAVSAAITKILNGTDPNTALDEAQKNVEFAMR"
     gene            2315..3247
                     /locus_tag="PLF56_01700"
     CDS             2315..3247
                     /locus_tag="PLF56_01700"
                     /inference="COORDINATES: protein motif:HMM:NF012738.2"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_function: GO:0042626 - ATPase-coupled transmembrane
                     transporter activity [Evidence IEA];
                     GO_function: GO:0140359 - ABC-type transporter activity
                     [Evidence IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="sugar ABC transporter permease"
                     /protein_id="HOQ52307.1"
                     /translation="MSLTLARPKQTGSGRDRRIRLEQGRNLTGWLFLIPAALLIGWMS
                     FWPMFQAFVLSLQTGRGTRLNWAQPLWYNYQRLFSDDIFKLTLENTLIYLVVQVPIML
                     ILALILANLLNDRNLRFKGLWRTAIFLPCAVSLVSYSLVFRTMFATDGLINDMLLGLH
                     LVDSPVNWLGETATARAVIIIGLLWRWTGYNMVFYLAALQNLDYSTIEAAKIDGAGSF
                     RTFWHVTVPQLKPMILLTAIMSTNGTIQLFDESWNLTQGGPAYTSMSMSHYLFELSFQ
                     KNPNFGYAAALSYAILIIVALLAAIQMKVGDKRD"
     gene            3240..4049
                     /locus_tag="PLF56_01705"
     CDS             3240..4049
                     /locus_tag="PLF56_01705"
                     /inference="COORDINATES: protein motif:HMM:NF012738.2"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_process: GO:0008643 - carbohydrate transport [Evidence
                     IEA];
                     GO_process: GO:0055085 - transmembrane transport [Evidence
                     IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="carbohydrate ABC transporter permease"
                     /protein_id="HOQ52308.1"
                     /translation="MTRSMRRLPGYAFLTIAAIVSVFPLYYMVVSATNTSADVLNSRM
                     LPGNNLWQNWADLTGAQNVASAMYYSMVNAVATTVLSLIVCSIAGYGFEVYHSRGKDR
                     VMSVLLLAMMIPFAATMIPLFQMFAQFGLVNSTVAVILPAIATPFLVLLFRQSARSFP
                     HEIIDAARMDGLSELAIFWRIFLPTMKSTYAAAAVITFMTAWNNFLWPKVILVSNTYQ
                     TMPMLLSNLRAGYVTNYGSLMLGVLIASVPTMVLFLVLQRSFAEGITGAIK"
     gene            4046..7111
                     /locus_tag="PLF56_01710"
     CDS             4046..7111
                     /locus_tag="PLF56_01710"
                     /inference="COORDINATES: protein motif:HMM:NF014850.2"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_function: GO:0004553 - hydrolase activity, hydrolyzing
                     O-glycosyl compounds [Evidence IEA];
                     GO_process: GO:0005975 - carbohydrate metabolic process
                     [Evidence IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="glycoside hydrolase family 2 TIM barrel-domain
                     containing protein"
                     /protein_id="HOQ52309.1"
                     /translation="MTPDLTHLDAAPDRGPGFDPSRIADPRYFGEHRLAPHSDHRWFA
                     TADEASSGLSSFEQKLSGLWKFHYAKNPAGAPSGFEGVDFDCSSWDDIAVPGHLQLQG
                     YGRPQYVNVQYPWDGHESVRPGQVPQRFNPVGSYVTWFTLERPLAEGERVSVSFQGAE
                     SAVAVWLNGTYVGYGADSFTPSEFDLTDALVEGENKLAAQVFTWTSGSWLEDQDFYRF
                     SGLFRDVMLYRRPAVHAEDLTVRTEVSDDLACAVVNVQVALTGAGSVRARIEGVGELA
                     DDGSSGLTVHLHDPRLWSAEDPYLYDLVIEVLDSSGAVVEVIPQPLGVRRFGIENGVL
                     SINGRRVVFRGVNRHEFGLNGRVMTRQQTEHDLALLKANNVNAVRTSHYPNNSFFYEL
                     CDRYGLYVIDEMNLETHGYWDALIRGVIPIDEVIPGDRPEWRAVLLDRAASMVERDKN
                     HPSIVLWSCGNESFGGTGIRDVADWFRQRDDTRPVHYEGVHWDPRYPDTTDVVSRMYA
                     PVTEIEQYLAEHRTKPYILCEYAHNMGNSFGAVDEYLALAYRDELFQGGFIWDFADQG
                     IALVDRYGQRYIGYGGDCGEAPHDGDFSANGMLFADHSPKPAVHEMKFLYQGVAIEVG
                     RGSFELTNRLLFTPSSVYECVVRLAREGSVLREAVVETHVAPGASAVYGLPFALPRGA
                     GEYTVDVSLQLRAATGWAASGHEVAFGQGVFVVEAPLVGRVETPDAPEVIDGIHNIGV
                     RGRHFHALFSKVHGGLLSYRYGLTRDGGNELLKAVPQPIFWHAPTSNERGWGMPARDG
                     QWLLASRYPMLDRDQPNPSLRLDGGVAELGYEYLLPTVPQSRCSVTYRVDGDGRVEVT
                     VRLQPGQGLSDLPEFGMLFTAPADLHRLTWYGEGPEECYVDRRAGARLGVYSGEVATQ
                     LTPYVRPQEAGSHTGVRWASVTDDRGLGLRFECAQGMEFSALPWTPFEIENARHPNEL
                     PPIHSTVLRPTLMRRGVGGDDSWGAMPLPAYLLPQGELGFTFAFRGVLG"
     gene            7589..8344
                     /locus_tag="PLF56_01715"
     CDS             7589..8344
                     /locus_tag="PLF56_01715"
                     /EC_number="3.4.-.-"
                     /inference="COORDINATES: protein motif:HMM:NF014566.2"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_component: GO:0016020 - membrane [Evidence IEA];
                     GO_function: GO:0004222 - metalloendopeptidase activity
                     [Evidence IEA];
                     GO_process: GO:0071586 - CAAX-box protein processing
                     [Evidence IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="CPBP family intramembrane metalloprotease"
                     /protein_id="HOQ52310.1"
                     /translation="MTTTSNPHDRTPVSAAPDFPFYDDRPVHVSGSRWLLVMGGVVLG
                     AAADLFVVVPGPGWLGMLVRAVLFAGIPLTVYALAIPGHWKSIFRRVGLRDVGLMVGL
                     AVVNVAVTFGIGLLLTTAMHLSPNPASGELRDMSLGDRLLTFASMVPQLFGEEVFTVL
                     PLLAILWLAVNKLHLSRRAGIAIAWIGSAVIFALLHLPTYNWNLLQCLLIIGTARLIL
                     SVAYLLTKNIWVSTGAHVLNDWTLFGLGLLAAH"
     gene            complement(8477..8911)
                     /locus_tag="PLF56_01720"
     CDS             complement(8477..8911)
                     /locus_tag="PLF56_01720"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_011756036.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="hypothetical protein"
                     /protein_id="HOQ52311.1"
                     /translation="MAATRRGEQRPATERDIHEVALGMPYVTIERGSSGLPVYQVGRT
                     SFVFFRTPRRDAVDPVTGERYDDVVVFWVAGEAEKQGLVQDDSTPFFTTAHFDGHPSV
                     LLRLSRVGELTRAELTEVVEDAWLAQASQRRRTNWLREHEAG"
     gene            complement(8915..9313)
                     /locus_tag="PLF56_01725"
     CDS             complement(8915..9313)
                     /locus_tag="PLF56_01725"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_011209367.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="VOC family protein"
                     /protein_id="HOQ52312.1"
                     /translation="MRINLTSVFVDDQSAARAFYTEVLGFQVRHDIPLGDASWLTVVS
                     PEAPEGTELLLEPSGHPAVKPYRDALVEDGIPLAQFAVDDLQAEHERLTGLGVTFTQP
                     PTDIGNAWVAVFDDTCGNLIQLVAMKPDEG"
     gene            complement(9310..9594)
                     /locus_tag="PLF56_01730"
     CDS             complement(9310..9594)
                     /locus_tag="PLF56_01730"
                     /inference="COORDINATES: protein motif:HMM:NF024248.2"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_function: GO:0003677 - DNA binding [Evidence IEA];
                     GO_function: GO:0003700 - DNA-binding transcription factor
                     activity [Evidence IEA];
                     GO_process: GO:0006355 - regulation of DNA-templated
                     transcription [Evidence IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="metalloregulator ArsR/SmtB family transcription
                     factor"
                     /protein_id="HOQ52313.1"
                     /translation="MADVFHALDDATRRLIMDELARHDAQSLFELCTALTMRHGLGST
                     RQAISQHLAVLEAAGLVRTERRGRTKLHHFDREPLAAITERWPQAGEDHP"
     gene            9791..10657
                     /locus_tag="PLF56_01735"
     CDS             9791..10657
                     /locus_tag="PLF56_01735"
                     /inference="COORDINATES: ab initio
                     prediction:GeneMarkS-2+"
                     /note="Derived by automated computational analysis using
                     gene prediction method: GeneMarkS-2+."
                     /codon_start=1
                     /transl_table=11
                     /product="hypothetical protein"
                     /protein_id="HOQ52314.1"
                     /translation="MEPVQTTIPGALAATLQARSRSLARRLLAGAGRASLVAYDLAPM
                     VEAGALCHGVSASGDLVVAGLRDAAVPATIWHDTPLRVRLDVVKEAPEWAVRITACAV
                     HLLGQLQWVPDDQVADYLLDAALPAGLIELGSSPGGRVGIVRADRVLVHDSAGVTPLP
                     FDAVVSRGCEAHEKGCGSFPDAEQEWAVRDVVGTLAPWQAMGLIQAAALGWTSAAMLS
                     TRGESACPHLEGQVFCVDVDRTGLTLMAVHQGEATVVFFAFARPVNHVDELADRLDQL
                     IEEGEAVRPPSR"
     gene            complement(10638..>10759)
                     /locus_tag="PLF56_01740"
     CDS             complement(10638..>10759)
                     /locus_tag="PLF56_01740"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_017934690.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=3
                     /transl_table=11
                     /product="DUF1540 domain-containing protein"
                     /protein_id="HOQ52315.1"
                     /translation="DGQVGACKRLECVHNSDLLCTAGKIDVGDAADCLTYSVA"
BASE COUNT         1718 a         3630 c         3595 g         1816 t
ORIGIN      
        1 cgcgcactcc agtcgccccg gaccagtgcc ggcgcgagca cccccgcctc gtccaggcag
       61 cgttgccagg tggcggcgcg caccagggcc ggctcggagt cggatggccc cgcgaggtgg
      121 tggacgatgc ggtggccgag ccccagcagg tgccgggtgg cggcctcact gccctgcacc
      181 tggtcggcga ccaccgaagg gtagtgaccg atcagtcgcg agtcggacac agccaccgcc
      241 atacccgccg gcagcgccag cgaatcgggc gtggcctgct cggcccgaat gatcaccagg
      301 ccgtcgatcg cctggtgcga cagccggagc gcggcatgct cccattgatc ggcggccggg
      361 gtgcgcacgt tcatcagggt caccgacagc ccctcggact cggcagcctc gaccaccgcc
      421 tcggtggtca gcgcctcgcc ggtgcgttcg aagcggtgcg cgatcagccc gatcgtgccg
      481 aacgacccgc tgcgcaacgc gcgcgccgcc cggttgggcg agtagcccag ctggtccatc
      541 gcctgcagca ccttcgcctt cgtttcgtca cggacggctt cggcgccggt cgacacgcgc
      601 gacacggtct gggcggagac cccggccagc cgggcgacgt cctcgatgga cgggccggtc
      661 ttgcgccggc ctcgccgggt ggtgctgggc atgtcgtcga tgctagtccg tggagctggc
      721 aatggctacg tgaacatccg gtccggggct ggcctcccat gccgtggaat gccgttattg
      781 aaccgtgatc tagcaggggt ggcggcgcgt cggtccgggt gctacgtata cgtaaacatt
      841 cgtcccgacc aatcggtggg accctcgggt aagcaccgac ccgcacctcg gtgatcgcgc
      901 accaacgaag gacgcactcg atgaacagat tctccaagat ggccgcactg gcggtcaccg
      961 cgctgctcag cgtgggcatg gccgcctgca gcggcggcgc caccgccacc agcccggccg
     1021 ccacctcagc cacgtccacc ggccccaaga gcggcaagct caccgtgtgg gcctgggacc
     1081 cgaccttcaa catctacgcg atgaaagagg ccgagaagat ctaccagaag gacaacccgg
     1141 gtttctcgct cgagatcgtc gagacaccct gggacgacct gcagaccaag ctcaccacgc
     1201 tcgcccagtc caaagagacc aagcagctgc ccgacatctt cctgatgcag aacaacgcgt
     1261 tccagaagaa cgtgatcaac taccccgacc tgttcagtga ctacgccaag tcgggcgtcg
     1321 acttcagcca gttccccgcc ggtgtcaccg cctactccac ggtcgacaac aagaactacg
     1381 gcctgccctt cgacaacggc accgcgatcc aggcgctgcg caccgatgtg atcaaagagg
     1441 ccgggtacac catggccgac ttcaccgaca tcacctggag cgacttcctg accaaggcga
     1501 aggcggtcaa ggccaagacc ggcaaggcgt tgctgtcggg ccaggccggg tcgtccgacc
     1561 tgatcatgat gatgctgcag agcgccggcg ccagcctgtt cgacgccgag ggtaagccga
     1621 cgatcaccaa caacccggcg ctgctcaaat ccatcgacgt ctacaagcaa ctcgtcgacg
     1681 ccaaggtgct caccgaggtg aacagctggg acgagtacat cggcaccttc gtcaacggca
     1741 ctgtggcggg caccatcaac ggcgtgtgga tcatgggttc gatccagacc gccaaggacc
     1801 agtccggcaa gtggcagatc accaacctgc ccaagctcga cggcgtcgag ggcgcgacca
     1861 actactcggc caacggcgga tcgtcctggg cgatcagctc caacgccaac tacgacctcg
     1921 cggccgactt cctgaacaag accttcgccg gctcgaagcc gctgtacgag acgattctgc
     1981 ccagctcggg tgcggtcgcg aactggctgc cgatggccga cagcaccgtc tactcccagc
     2041 cgcagaagtt cttcggtgac cagcccgtgt tcagcaaggt gatcgagttc agcaagcagg
     2101 tgcccaagaa caacaccggc gcctactact acgagggccg cgacgccgtg agcgctgcga
     2161 tcaccaagat cctcaacgga accgacccca acacggcact cgatgaggct cagaagaacg
     2221 ttgagttcgc gatgcgctga gcgcgcccca tcaggtgatc ggtgggtggg tggccggggg
     2281 accggccgcc cactccccac agaaaggacg caccgtgtcg ctcaccctcg cacgccccaa
     2341 acagaccggt tccggacgcg accggcgcat ccgtttggaa cagggccgca acctcaccgg
     2401 ctggctgttc ctgattcccg ccgcgctact gatcggctgg atgagtttct ggcccatgtt
     2461 tcaggcgttc gtgttgtcgc tgcagaccgg acgtggcacc cggctcaact gggcccagcc
     2521 gctctggtac aactaccagc gcctgttctc ggacgacatc ttcaagctga ccctcgagaa
     2581 cacgctgatc tacctggtgg tgcaggtgcc gatcatgctg attctggccc tgatcctcgc
     2641 caacctgctc aacgaccgca acctgcgctt caagggcctg tggcgcaccg cgatctttct
     2701 gccgtgcgcg gtgtcgctgg tcagctactc cctggtcttt cgcaccatgt tcgccaccga
     2761 cgggctgatc aacgacatgc tgctgggcct gcacctggtc gactcgccgg tcaactggct
     2821 gggtgagacc gcgaccgccc gggccgtgat catcatcggc ctgctgtggc gctggaccgg
     2881 ctacaacatg gtgttctacc tggccgccct gcagaacctc gactactcga cgatcgaggc
     2941 ggccaagatc gacggcgcgg gttcgttccg caccttctgg cacgtcaccg tgccacaact
     3001 caaaccgatg atcctgttga ccgcgatcat gtcgaccaac ggcaccattc agctgttcga
     3061 cgagtcgtgg aacctcaccc agggcggccc cgcctacacc tcgatgtcga tgtcgcacta
     3121 cctgttcgaa ctgtcgtttc agaagaaccc caatttcggt tatgccgcgg cattgtcgta
     3181 cgcgatcctg atcatcgtcg cgctgctggc cgccattcag atgaaggtgg gtgacaagcg
     3241 tgactagaag catgcggcgc ctgcccggct atgcgttctt gaccatcgct gccatcgttt
     3301 cggtctttcc gctgtactac atggtggtct cggccaccaa caccagcgcc gacgtgctca
     3361 acagccgcat gctgccgggc aacaacctgt ggcagaactg ggccgacctg accggagccc
     3421 agaatgtcgc ctcggccatg tactactcga tggtgaacgc ggtggccacc accgtgctgt
     3481 cgctgatcgt gtgctcgatc gcgggctacg gcttcgaggt gtaccactct cggggcaagg
     3541 accgggtgat gagcgtgctg ctgctggcga tgatgattcc gttcgcggcc accatgattc
     3601 cgctgtttca gatgttcgcc cagttcggcc tggtcaactc gacggtcgcg gtgattctgc
     3661 cggccatcgc cacgccgttc ctggtgttgc tgtttcggca gtcggcgcgt tcgtttccgc
     3721 acgagatcat cgacgcggca cgcatggacg ggctgagcga actggccatt ttctggcgca
     3781 tcttcctgcc caccatgaaa tcgacctatg ccgcggcggc ggtgatcacc ttcatgaccg
     3841 cctggaacaa cttcctgtgg cccaaggtga ttctggtcag caacacctac cagacgatgc
     3901 cgatgctgct gtcgaacctg cgcgccggct acgtcaccaa ctacggctcg ctgatgctgg
     3961 gcgtgctgat cgcctcggtg cccaccatgg tcttgttcct ggtgctgcag cgcagcttcg
     4021 ccgaaggaat cacaggagcc atcaagtgac acccgacctg acccacctcg acgccgcacc
     4081 cgaccgcggt ccgggcttcg acccgtcccg catcgccgat ccccgctact tcggcgagca
     4141 tcggctggcg ccgcattccg atcaccgctg gttcgccacg gcggacgagg cgagcagcgg
     4201 gctcagcagc ttcgaacaga agttgagcgg actgtggaag ttccactacg ccaagaaccc
     4261 ggccggggca ccgtccggct tcgaaggggt cgacttcgac tgctcgagct gggacgacat
     4321 cgcggtgccg ggccacctgc aactgcaggg ctacggacgc ccgcagtacg tcaacgtgca
     4381 gtacccgtgg gacggccatg agagcgtgcg gcccgggcag gtgccgcaac ggttcaaccc
     4441 ggtcggcagc tacgtcacct ggttcaccct tgagcgtccc ctggccgagg gcgagcgggt
     4501 cagcgtgtcg ttccagggcg ccgagagcgc ggtggcggtc tggctcaacg gcacctacgt
     4561 cggttatggg gccgactcgt tcacgccgtc cgagttcgac ctgaccgacg cgctggtcga
     4621 gggcgagaac aagctggctg cccaggtgtt cacctggacc tcgggctcgt ggctcgaaga
     4681 ccaggacttc taccggttct cggggctgtt ccgtgatgtg atgctgtacc ggcgtccggc
     4741 cgtgcatgcc gaagacctga cggtgcgcac cgaggtgtcc gacgacctcg cctgcgccgt
     4801 ggtgaacgtg caggtcgcgc tgaccggtgc gggcagcgtc cgggcgcgca tcgaaggggt
     4861 cggcgaactc gcggacgacg ggtcgagcgg gctgactgtg cacctgcacg acccgcggct
     4921 gtggagtgcc gaggatccct acctgtacga cctggtgatc gaggtgctgg attcgtccgg
     4981 cgcggtggtc gaggtgatcc cgcagccgct cggcgtgcgc cggttcggca tcgagaacgg
     5041 tgtgctgtcg atcaacgggc gccgggtggt gttcaggggt gtcaaccggc acgagttcgg
     5101 cctgaacggc cgggtgatga cccggcagca gaccgagcac gaccttgccc tgctgaaggc
     5161 caacaacgtc aacgcggtac gcaccagcca ctacccgaac aactcgttct tctacgagct
     5221 ctgcgaccgc tacggcctgt atgtgatcga cgagatgaac ctcgagaccc acggctactg
     5281 ggacgccctg atccgcggtg tgatcccgat cgatgaggtg ataccgggcg atcgtccgga
     5341 gtggcgcgcg gtgctgctcg accgggccgc cagcatggtg gagcgcgaca agaaccaccc
     5401 gagcatcgtg ctgtggtcgt gcggcaacga gtcgttcggg ggcaccggca tccgcgacgt
     5461 ggccgactgg ttcaggcagc gggacgacac ccggccggtg cactacgaag gggtgcactg
     5521 ggacccgcgg taccccgaca ccaccgacgt ggtgagccgc atgtacgcgc cggtgaccga
     5581 gatcgagcag tacctggccg agcaccgcac caagccctac attctgtgcg agtacgccca
     5641 caacatgggc aactcgttcg gtgcggtcga tgaatacctg gccctggcct accgcgacga
     5701 gctgtttcag ggcgggttca tctgggactt cgccgaccag ggcatcgcgc tggtcgaccg
     5761 gtacggccag cggtacatcg ggtacggcgg cgactgtggc gaggctccgc acgacggcga
     5821 cttcagcgcc aacggcatgc tgttcgccga tcattcgccc aagccggccg tgcacgagat
     5881 gaagtttctc taccagggcg tcgcgatcga ggtcggacgg ggctcgttcg agctgaccaa
     5941 ccggctgctg ttcaccccgt cgtccgtgta cgagtgcgtg gtgcgattgg cccgcgaggg
     6001 ttcggtgctg cgcgaggcgg tcgtcgagac ccacgtggcg ccgggggcgt ccgcggtgta
     6061 cgggttgccg ttcgcgctgc cccgcggtgc gggggagtac accgtggacg tgtcgctgca
     6121 gctgcgcgca gcgaccgggt gggcggcgtc cgggcatgag gtggcgttcg gccagggggt
     6181 gttcgtggtc gaggccccgc tggtcgggcg tgtcgagacc cccgacgccc ccgaggtgat
     6241 cgacggcatt cacaacatcg gcgtgcgcgg acggcacttt cacgccctgt tctccaaggt
     6301 gcacggagga ctgctctcgt accgctacgg actcacgcgc gatggtggca acgaactgct
     6361 gaaggcggtg ccgcagccca tcttctggca cgcacccacc tcgaacgagc gcggctgggg
     6421 catgccggcc cgcgatggtc agtggctgct ggccagccgc tacccgatgc tcgatcggga
     6481 ccagcccaac ccgtcgttgc gactggacgg tggcgtggcc gagctggggt atgagtacct
     6541 gctgccgacc gtgccgcaga gccgttgctc ggtcacctac cgggtcgacg gtgacgggcg
     6601 ggtcgaggtc acggtcaggc tgcagccggg gcagggactt tccgacctgc ccgagttcgg
     6661 catgctgttc accgcgccgg ccgacctgca ccggctgacc tggtacggcg agggtcctga
     6721 ggagtgttac gtcgaccggc gcgccggcgc ccggctcggg gtctactccg gtgaggtggc
     6781 gacgcaactg acgccgtacg tacgtccgca ggaggccggc agccacaccg gcgtccgctg
     6841 ggccagcgtg acggacgatc gggggctcgg cctgcgcttc gagtgcgctc agggcatgga
     6901 gttctcggcc ctaccctgga cgccgttcga gatcgagaac gcgcggcacc ccaacgagtt
     6961 gccgccgatc cactccacgg tgctgcggcc gacgctgatg cgtcgcgggg tgggcggcga
     7021 tgactcctgg ggtgcgatgc cgctgccggc gtacctgctg ccgcaaggtg agctgggctt
     7081 cacgttcgca ttccggggcg tgctgggcta ggaagcgctg gtcgagccgg tcgagaccgc
     7141 tgcgaccagg ggtttcgacg ctcaaccagc ggtgcggctc ctgaggccag gctataggag
     7201 cgtgtcgtcc acccccggcc gcgctgcggc gcacccggta cgcccgctcg ctgcgtggtc
     7261 atcctcggcg atcgggccca ccggtccgtg caagggcaac cggatccacc ttgcgcgcgg
     7321 ccggcgccgc cggggcgcct gcgcgagcat cgatggatca gcgtgcctct cgcgggccga
     7381 ccgggtgacc cgcccacccg catgagggag ggttcggtac gggcacggcg cggttcggcg
     7441 cctgaacgtc ctgggcacac ttgcctcgga gtatgcccac gttcatccgt acggatggtg
     7501 gcagcagcgt ccagagcgag gatccttgac ctgtcaccgc gaacgggcgg aacgtcgtac
     7561 agaaccgcga gaggcgcaca acacacacat gaccaccacc agcaatcccc acgaccgaac
     7621 acccgtttcg gccgctcccg actttccctt ctacgacgac cggccagtgc acgtcagcgg
     7681 gtcgaggtgg ctgctggtga tgggcggtgt cgtgctcggc gcggcggccg acctgttcgt
     7741 cgtcgtaccg gggccgggct ggctcggcat gctggtccgc gccgtgctgt tcgccggcat
     7801 tccgctcacc gtgtacgccc tggcgatccc gggccactgg aagtcgatct tccgccgggt
     7861 cggcctgcgc gacgtcggcc tgatggtggg cctcgcggtg gtgaacgtgg cggtcacctt
     7921 cggcatcggc ctgctgctca ccacggccat gcacctgagc cccaacccgg cgagcggcga
     7981 actgcgcgac atgtcgctgg gcgaccggct gctcaccttc gcttcgatgg tgccgcaact
     8041 cttcggcgaa gaggtgttca ccgtccttcc gctgctggcc atcctgtggc tcgcggtcaa
     8101 caagctgcac ctgtcccggc gggccggcat cgcgatcgcc tggatcggca gcgcggtgat
     8161 cttcgcgctg ctgcacctgc cgacctacaa ctggaacctg ttgcagtgcc tgctgatcat
     8221 cggcaccgcc cggttgattc tcagcgtcgc ctacctgctc accaagaaca tctgggtctc
     8281 cacgggcgcc cacgtgctca acgactggac gctgttcggc ctgggcctgc tggccgcaca
     8341 ctgaacggca gttgggtaac gggggcagcc gtccgcggga aggccgccga cccgacatcg
     8401 tgccgggtgg atcgttcccg atcggccacg ccgcgtccgc tcaactcggc ttcaccagcc
     8461 ggacgcggtg gccgattcag cccgcttcat gctcgcgcag ccagttggtc cgccgccgct
     8521 gcgacgcctg cgccagccac gcgtcctcga ccacctcggt gagttcggcg cgggtgagtt
     8581 cgcccaccct gctgagccgc agcaggacgg acgggtgccc gtcgaagtgg gcggtggtga
     8641 agaacggcgt cgagtcgtcc tgcaccaggc cctgcttctc ggcctcgccg gccacccaga
     8701 acaccaccac gtcgtcatag cgctcaccgg tcaccgggtc gacggcgtcc ctgcggggcg
     8761 tgcgaaagaa gacgaacgac gtgcggccga cctggtacac cggcagtccg gacgaccccc
     8821 gctcgatcgt gacgtacggc atgccgagcg ccacctcgtg gatgtcgcgc tcggtcgccg
     8881 gacgttgctc gccccggcgc gtcgcagcca ccggtcaacc ctcgtcgggc ttcatcgcca
     8941 ccagttggat caggttgccg caggtgtcgt cgaacacggc cacccaggcg ttgccgatgt
     9001 cggtcggcgg ctgggtgaag gtgacgccca acccggtcag tctttcgtgc tcggcctgaa
     9061 ggtcgtcgac ggcgaactgg gcgagcggaa tgccgtcctc gaccagcgcg tcgcggtacg
     9121 gcttgaccgc cgggtgcccg gacggctcca gcagcagttc ggtgccctcc ggcgcctcgg
     9181 gcgagaccac ggtcagccac gacgcgtcgc cgagcggaat gtcgtgccgc acctgaaagc
     9241 cgagcacctc ggtatagaag gcgcgggcgg ccgattgatc gtcgacgaag acgctggtca
     9301 ggttgatcct catgggtggt cctctccggc ctgcggccag cgttcggtga tcgccgcgag
     9361 cggttcgcgg tcgaagtggt gcagtttggt gcggccccgc cgttcggtgc gcaccagacc
     9421 ggccgcctcg agcaccgcga ggtgctgcga gatcgcctgc cgggtcgatc cgagtccgtg
     9481 ccgcatggtg agcgcggtgc agagttcgaa cagcgactgc gcatcgtgtc tcgccagctc
     9541 gtccatgatc aggcgcctgg tcgcatcgtc gagagcgtga aagacgtctg ccacccggcg
     9601 atcataggca agtagctgct tgcctatcaa gacggggggt ctgatcgcgc tccggagccg
     9661 gtcgatgggt gagggcgtcc atgctgggcg ggatgggctg tgtcgaccgc atgtttaggt
     9721 taggctcccc ttacagacgc cgcgatggtg tcgctcgctc cgtccgccca tttcacactc
     9781 agggggtcct gtggaacccg tccagaccac gatcccaggc gcgctcgccg ccaccctgca
     9841 ggcccgctcg cggtcgctgg cgcgtcggct gctcgccggt gccggacgtg cctcgctggt
     9901 ggcctacgac ctggcgccga tggtcgaggc gggcgccctg tgtcacggcg tcagcgcgag
     9961 cggcgatctg gtggtcgccg gcctgcgcga cgcggccgtg cccgccacga tctggcacga
    10021 caccccgctg cgggtacggc tcgatgtggt gaaagaggcg cccgagtggg cggtgcgcat
    10081 cacggcctgc gcggtgcacc tactcggcca gctgcagtgg gtgccggacg atcaggtggc
    10141 tgactacctg cttgatgccg cgcttccggc cggactgatc gaactcggct cgtcgcccgg
    10201 tggacgagtg ggcatcgtgc gcgccgatcg ggtgctcgtg cacgacagtg caggggtgac
    10261 gccgctgccc ttcgatgcgg tggtctctcg cgggtgcgag gcgcacgaga agggctgcgg
    10321 gagttttccc gatgccgaac aggaatgggc ggtgcgtgac gtggtcggca cgctcgcacc
    10381 gtggcaggcc atgggtctga tacaggcggc ggcgctgggc tggacctcag cggcgatgct
    10441 gtcgacgcgg ggcgagagcg cgtgcccgca tcttgaaggt caggtgttct gcgttgacgt
    10501 ggaccgcacc ggactgaccc tcatggcggt tcaccaaggc gaggccacgg tggtgttctt
    10561 cgcgtttgcg cggccggtga accacgtcga tgagctggcc gatcgactcg accagctcat
    10621 cgaagaaggc gaagcggtca ggccaccgag taggtgaggc agtcggcggc gtcgccgacg
    10681 tcgatcttgc cggcggtgca cagcaggtca ctgttgtgca cacactcgag gcgcttgcag
    10741 gcgcccacct gaccgtcgg
//
LOCUS       DAOSUG010000027        10672 bp    DNA     linear   ENV 10-JUN-2024
DEFINITION  MAG TPA_asm: Micropruina sp. isolate AMR_MDS_2195
            NODE_3456_length_10672_cov_4.029293, whole genome shotgun sequence.
ACCESSION   DAOSUG010000027 DAOSUG010000000
VERSION     DAOSUG010000027.1
DBLINK      BioProject: PRJNA925477
            BioSample: SAMN32864262
            Sequence Read Archive: SRR4236673
KEYWORDS    WGS; ENV; Metagenome Assembled Genome; MAG; Third Party Data; TPA;
            TPA:assembly.
SOURCE      Micropruina sp. (wastewater metagenome)
  ORGANISM  Micropruina sp.
            Bacteria; Bacillati; Actinomycetota; Actinomycetes;
            Propionibacteriales; Nocardioidaceae; Micropruina.
REFERENCE   1  (bases 1 to 10672)
  AUTHORS   Abdulkadir,N., Saraiva,J.P., Zhang,J., Stolte,S., Gillor,O.,
            Harms,H. and Rocha,U.
  TITLE     Genome-centric analyses of 165 metagenomes show that mobile genetic
            elements are crucial for the transmission of antimicrobial
            resistance genes to pathogens in activated sludge and wastewater
  JOURNAL   Microbiol Spectr 12 (3), e0291823 (2024)
   PUBMED   38289113
REFERENCE   2  (bases 1 to 10672)
  AUTHORS   Abdulkadir,N.
  TITLE     Direct Submission
  JOURNAL   Submitted (24-MAR-2023) Environmental Microbiology, Helmholtz
            Center for Environmental Research -UFZ, Permoserstr 15, Leipzig
            04318, Germany
COMMENT     The annotation was added by the NCBI Prokaryotic Genome Annotation
            Pipeline (PGAP). Information about PGAP can be found here:
            https://www.ncbi.nlm.nih.gov/genome/annotation_prok/
            
            ##Genome-Assembly-Data-START##
            Assembly Method        :: SPAdes v. 3.11.1
            Genome Representation  :: Full
            Expected Final Version :: Yes
            Genome Coverage        :: Not Applicable
            Sequencing Technology  :: Illumina MiSeq
            ##Genome-Assembly-Data-END##
            
            ##Genome-Annotation-Data-START##
            Annotation Provider               :: NCBI
            Annotation Date                   :: 05/04/2023 04:57:10
            Annotation Pipeline               :: NCBI Prokaryotic Genome
                                                 Annotation Pipeline (PGAP)
            Annotation Method                 :: Best-placed reference protein
                                                 set; GeneMarkS-2+
            Annotation Software revision      :: 6.5
            Features Annotated                :: Gene; CDS; rRNA; tRNA; ncRNA
            Genes (total)                     :: 2,776
            CDSs (total)                      :: 2,750
            Genes (coding)                    :: 2,714
            CDSs (with protein)               :: 2,714
            Genes (RNA)                       :: 26
            tRNAs                             :: 24
            ncRNAs                            :: 2
            Pseudo Genes (total)              :: 36
            CDSs (without protein)            :: 36
            Pseudo Genes (ambiguous residues) :: 0 of 36
            Pseudo Genes (frameshifted)       :: 2 of 36
            Pseudo Genes (incomplete)         :: 32 of 36
            Pseudo Genes (internal stop)      :: 2 of 36
            ##Genome-Annotation-Data-END##
FEATURES             Location/Qualifiers
     source          1..10672
                     /organism="Micropruina sp."
                     /mol_type="genomic DNA"
                     /submitter_seqid="NODE_3456_length_10672_cov_4.029293"
                     /isolate="AMR_MDS_2195"
                     /isolation_source="Activated sludge of municipal
                     wastewater treatment plants"
                     /db_xref="taxon:2737536"
                     /environmental_sample
                     /geo_loc_name="Canada"
                     /lat_lon="49.25 N 123.1 W"
                     /collection_date="2015-06-16"
                     /metagenome_source="wastewater metagenome"
                     /note="metagenomic"
     gene            <1..901
                     /locus_tag="PLF56_01745"
     CDS             <1..901
                     /locus_tag="PLF56_01745"
                     /inference="COORDINATES: ab initio
                     prediction:GeneMarkS-2+"
                     /note="Derived by automated computational analysis using
                     gene prediction method: GeneMarkS-2+."
                     /codon_start=2
                     /transl_table=11
                     /product="hypothetical protein"
                     /protein_id="HOQ52316.1"
                     /translation="TGFVLLGRRAWATTLRRGTETPSTRTAGALALFASWLVINIAVF
                     SFMAGIYHDYYTVALAPAIGGTLALGGTLAWARRDTWLGRAALATAALATGAWGIALA
                     RVAGGVYLTVSIAAAAVLAACAALFLFGRHLARTLQALAISAAIVAGLAGPAAYSLNT
                     AATAHTGSIVTAGPARTGISGGSSMGGGGGPGGGGRAGGAGQGQPPGQNGNQTQPGGT
                     GQTGTGQTARGRPARARPAVAGASRAPPPVVAAVPAGCSTGRPSRPSCSTCSRRTPPT
                     TPGWRPPRAPRTPPATNSPRVMR"
     gene            838..1125
                     /locus_tag="PLF56_01750"
     CDS             838..1125
                     /locus_tag="PLF56_01750"
                     /inference="COORDINATES: ab initio
                     prediction:GeneMarkS-2+"
                     /note="Derived by automated computational analysis using
                     gene prediction method: GeneMarkS-2+."
                     /codon_start=1
                     /transl_table=11
                     /product="hypothetical protein"
                     /protein_id="HOQ52317.1"
                     /translation="MAATTGAQNAASYQLASGDAVMAIGGFNGSDPSPTLEQFKAHVA
                     AGEIHYFIGGGNFGGQNGGSGAASEIASWVQQNYTATTVGGTTVYDLTATK"
     gene            1133..>2313
                     /locus_tag="PLF56_01755"
     CDS             1133..>2313
                     /locus_tag="PLF56_01755"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_008355793.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="bifunctional glycosyltransferase family 2/GtrA
                     family protein"
                     /protein_id="HOQ52318.1"
                     /translation="MTMIENRDLVRRSSATKHAVVEIVVPVYNEADALEASIRRLAAF
                     LDDELPYRFQVTIADNASTDATWTIANRLADQVQGVHVVHLDAKGRGRALKQVWLAST
                     ADVVAYMDVDLSTDLAALLPLLAPLVSGHSDVAIGSRLARGSRVVRGPKREFISRCYN
                     LILRGALQVSFSDAQCGFKAMRTDVARRLLPLVQDTNWFFDTELLVLAQRAGLRIHEV
                     PVDWVDDPGTTVDIAATASEDLRGVWRLLRGLTRGDIPIAVVREQLARPALPATVVPQ
                     GLLGQVLRFGAVGVASTLAYFVLYLVLRLGLDAQVANLTALALTAVANTAANRVFTFG
                     VRGNDGWLTHHVTGLVAFAVALGLTSGSLALLHGLAPEAGRFVEVMVLAVANAVATAV
                     R"
     assembly_gap    2314..2413
                     /estimated_length=100
                     /gap_type="within scaffold"
                     /linkage_evidence="paired-ends"
     gene            2881..3834
                     /locus_tag="PLF56_01760"
     CDS             2881..3834
                     /locus_tag="PLF56_01760"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_012918658.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="asparaginase"
                     /protein_id="HOQ52319.1"
                     /translation="MVNVGDAPVVAQVVRNGFVESTHRGLVVITRADGSVSYSRGDYN
                     SHILPRSSLKPIQALAMVRCGLDVPADQLAIACASHSGEPMHLQTVLDLLHGAGLSES
                     DLRNTPDLPLIDAQRNEWIAAGRPPSSLAQNCSGKHAAMLATCKLNDWPTGTYLNRGH
                     PCQQAVIETTRGFGQHVFWTSIDGCGAPAYAISLVGLARIFGILAAATEGAEKAVADA
                     MRAHPELVGGTGRDVTDLMRGVPGMIAKDGAEGVYAAGLPDGRGIAIKITDGGVRARP
                     VVMGAVMRRLWLGDESLRRRLMDMPIYGRGVPVGAVIPIDI"
     gene            complement(3951..4625)
                     /locus_tag="PLF56_01765"
     CDS             complement(3951..4625)
                     /locus_tag="PLF56_01765"
                     /inference="COORDINATES: ab initio
                     prediction:GeneMarkS-2+"
                     /note="Derived by automated computational analysis using
                     gene prediction method: GeneMarkS-2+."
                     /codon_start=1
                     /transl_table=11
                     /product="hypothetical protein"
                     /protein_id="HOQ52320.1"
                     /translation="MEIVLSLLRTLAFVVATVAATVYVARRIGRGRPLLWTGTTLLAL
                     YWLVLFGWTAYVAGSTSPNADLAIWVGWLGHLLPFVATLTYIWGIGQAAGFPSRAGAD
                     AGSRFGRRAPEPAAPPSGASAEPASTTPASDAGATPAVGTSNAGTHGAGASGTVNPAG
                     GTGSAPAVGAASGSTASTSATPPSAAKPKPALPFAGPHAVPVVTTPSAGSHHSPADPP
                     KTRGAK"
     gene            4787..6883
                     /locus_tag="PLF56_01770"
     CDS             4787..6883
                     /locus_tag="PLF56_01770"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_014847168.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_function: GO:0003924 - GTPase activity [Evidence IEA];
                     GO_function: GO:0005525 - GTP binding [Evidence IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="elongation factor G-like protein EF-G2"
                     /protein_id="HOQ52321.1"
                     /translation="MSSKVNATTMQVTSPDDVRNVVLIGNAGSGKTTLFEQLLKARVD
                     GYRGEKEDAERASQLFLASFVTGDTVVNLLDAPGHPDFVGELRAGIRAADAAIFVLSA
                     ADGIDGAAQALWEECREANLPRVIVLTKLDAGHADFDTSVEECQAVFGEGVRPTHVPT
                     FSGPNQVSGNIGLLSLEEHDYSGGAPVSRTLNAHDDQVETYRGPLIESIIQEAEDDGL
                     MDRYLEDDEHLDVNDLLDDLMKAVATANLFPVVPVQTGTGVGIEELLRLIEHGFPTPG
                     LHPNPSLTTTGGEVLPQPDTDPSAPLVAQVIRTTTDPFAGRLSMVRIFSGTLHTDDVV
                     HVSGHRSWSAGQSDNAHPDHDNEERIGLIQGAVGTELKPRQSAMAGEIVLLPKLGTAE
                     TGDTLSAKERPGVVEAWPLPDPLLPLAIRAATRNDEDKLAGALQRLAVEDSSVRLDRG
                     AGDQLVLWTTGQAHADLVLGRLVERYQVRVEQEDLKIPMRETFTGKADAMGRHVKQSG
                     GHGQYAVCNIEVEPLERGSGFEFVDKVVGGAVPRQFIPSVEKGIRTQLERGVFAGYPM
                     VDLRVTLYDGKSHSVDSSDMAFQMAGALAIKEAAAAKGGVVALLEPVDLVTVTVGEDY
                     LGAVMTDLSGRRGQVLGSDSVNRKAIVRALVPQAELTRYAIDLRGLGHGSGTFTREFH
                     GYELLPSNLLDKFAKK"
     gene            complement(6959..7813)
                     /locus_tag="PLF56_01775"
     CDS             complement(6959..7813)
                     /locus_tag="PLF56_01775"
                     /inference="COORDINATES: protein motif:HMM:NF016370.2"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="DUF559 domain-containing protein"
                     /protein_id="HOQ52322.1"
                     /translation="MERILELLHLEGGVLQRKRHRTLTRQLDQCIRRGRLRAILPGVY
                     TEPEPSWEARMRAAAAFRCDAVITGAAAARSLWWPECPITAVSAALSSGTVERAYSGF
                     SWERRSIPPELIVHRGDLRFVCPALSVLDLIPTLGGTVIDEALRRGSTTLPELWQGLH
                     RCPHRPGNTRRRLLLHDSRDAPWSEPERMAHQLLRAARIGGWRTNYRIRIGEITHFVD
                     IAFPQQRVAVEIDGWEHHGTRSAFVKDRWRYARLAAAHWIVLPFAAQVLTDEPDTFIE
                     LVRTAVGA"
     gene            complement(8043..8453)
                     /locus_tag="PLF56_01780"
     CDS             complement(8043..8453)
                     /locus_tag="PLF56_01780"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_012921034.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="YccF domain-containing protein"
                     /protein_id="HOQ52323.1"
                     /translation="MRTLLNIIWLVFGGFWLALGYFIFGILACILIITIPFGIASFRM
                     ASFALWPFGKTIVSKPTAGVGSALGNVIWFLVAGLWLAIGHLTTAAAQAITIVGIPLA
                     IANIKMIPVTCVPLGKEIVDSRQVPYGANAVYSF"
     gene            8950..9681
                     /gene="trmB"
                     /locus_tag="PLF56_01785"
     CDS             8950..9681
                     /gene="trmB"
                     /locus_tag="PLF56_01785"
                     /EC_number="2.1.1.33"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_014847173.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_function: GO:0008176 - tRNA
                     (guanine-N7-)-methyltransferase activity [Evidence IEA];
                     GO_process: GO:0006400 - tRNA modification [Evidence IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="tRNA (guanosine(46)-N7)-methyltransferase TrmB"
                     /protein_id="HOQ52324.1"
                     /translation="MDEPRARVQREVVSFVRRSARMNESQLKAWTHLQHWVVSVPTRQ
                     LSTSIAPEARVDWPAVFGREAPLVVELGGGTGDVIAAVAARHPDRDFIAFEVFEPAVA
                     STLSKCARAGVTNVRVVVANGVDGLTTLIDEAALSELWTFFPDPWHKARHHKRRLVSP
                     DFAAVAAHTLAPDGLWRLATDWEDYALAIRAVLDANPRLQNCHDGWAPRWQDRPQSRY
                     EAKGLAAGRTIYDLCYRRTRERRDP"
     gene            9678..10490
                     /gene="truA"
                     /locus_tag="PLF56_01790"
     CDS             9678..10490
                     /gene="truA"
                     /locus_tag="PLF56_01790"
                     /EC_number="5.4.99.12"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_013160539.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_function: GO:0009982 - pseudouridine synthase activity
                     [Evidence IEA];
                     GO_process: GO:0006400 - tRNA modification [Evidence IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="tRNA pseudouridine(38-40) synthase TruA"
                     /protein_id="HOQ52325.1"
                     /translation="MRLRLDIAYDGTEFHGWAGQPGLRTVQEELEAWIPRVLRLAEPV
                     TLTCAGRTDAGVHARGQVAHLDLPEPDDAERTVADLTYKLRNVLPPDVVVRRVSVAPP
                     GFDARFSAIWRRYVYRIADATPDPLLRIHVTPMRTELDLGRINALAPRLIGLHDFAAF
                     CKAREGATTIRNLQHLSARRTWGDVAEITVRADAFCHSMVRSLVGGLVAVATARREPD
                     WLIGLLGASERAGEIKVMPACGLTLEEVGYPPDRELAARAREARAVRTLESE"
     gene            10487..>10672
                     /locus_tag="PLF56_01795"
     CDS             10487..>10672
                     /locus_tag="PLF56_01795"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_016666064.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="methyltransferase"
                     /protein_id="HOQ52326.1"
                     /translation="MSHYFTAPTGPEKRRTLDVRFWDTDWRFTTANEVFSGDGLDLGT
                     AVLLRESTPPADATRLLD"
BASE COUNT         1606 a         3686 c         3731 g         1549 t
ORIGIN      
        1 caccggtttc gtgctgctcg gacgccgcgc ctgggccacc acgctgcgtc gcggcaccga
       61 gaccccgtcg acccggacgg ccggcgccct ggccctgttc gcgagctggc tggtgatcaa
      121 tatcgccgtg ttcagcttca tggccggcat ctaccacgac tactacacgg tcgcgctggc
      181 ccccgccatc gggggcacgc tggcactcgg cgggacgctc gcctgggccc gtcgcgacac
      241 ctggctcgga cgggccgcgc tcgccacggc cgccctggcc accggcgcgt ggggcatcgc
      301 gctggcccgg gtcgccggcg gcgtctacct gaccgtctcg atcgcggccg ccgccgtgct
      361 ggccgcctgt gcggcgctgt tccttttcgg gcgtcacctg gcccgtaccc tgcaggcgct
      421 ggcgatcagc gccgcgatcg tggccggtct ggccggtccg gcggcgtact cgctcaacac
      481 cgccgccacc gcgcacaccg gctcgatcgt caccgccgga ccggccagga ccggcatctc
      541 gggcggcagc agcatgggcg gcgggggcgg tcccggcggg ggtgggcgtg ccggtggcgc
      601 cggccagggc caaccgcctg ggcagaacgg caaccagacc caacccggtg gtacggggca
      661 gaccggtacc ggccagaccg cacggggcag accagcacgg gccagaccgg ccgtggcggg
      721 ggccagccga gcaccacctc ccgtggtggc ggcggtgccg gcgggctgct caacggggcg
      781 tccgtctcgt ccgagctgct cgacctgctc gaggcgaacg cctccgacta cacctgggtg
      841 gcggccacca cgggcgccca gaacgccgcc agctaccaac tcgcctcggg tgatgcggtg
      901 atggcgatcg gtggtttcaa cggctccgac ccgtcgccga ccctggagca gttcaaggcc
      961 cacgtggccg cgggcgagat ccactacttc atcggcggcg gcaacttcgg cggccagaac
     1021 ggcggcagcg gggcggcgtc cgagatcgcg agctgggtgc agcagaacta caccgccacc
     1081 accgtcggcg gaaccaccgt ctacgacctg actgcgacga agtgaggccg cgatgaccat
     1141 gatcgaaaac cgggacctgg tgcggcgcag ctccgccacc aagcatgccg tggtcgagat
     1201 cgtcgtgccg gtctacaacg aggcggacgc cctcgaggcg tccattcgcc ggctggcggc
     1261 ctttctggac gacgaactgc cctaccgctt tcaggtgacg atcgccgaca acgcgtccac
     1321 cgacgccacc tggacgatcg cgaaccggct ggccgaccag gtgcagggcg tgcacgtcgt
     1381 gcacctcgac gccaagggac ggggccgggc gctcaagcag gtgtggctgg ccagcacggc
     1441 cgacgtggtc gcctacatgg acgtggacct ctccaccgac ctggccgccc tgctgcccct
     1501 gctcgctccg ctggtctccg ggcattccga cgtcgccatc ggcagccggc tggcccgcgg
     1561 ctcacgggtg gtgcgtggcc ccaaacggga gttcatctcg cgctgctaca acctgatcct
     1621 gcgcggcgca ctgcaggtgt cgttcagcga tgcgcagtgc ggattcaagg ccatgcgaac
     1681 cgatgtcgcg cgccggttgc tgcccctcgt gcaggacacc aactggttct tcgacaccga
     1741 gctgctggtg ttggcccagc gggccggcct gcgcattcac gaggtgccgg tcgactgggt
     1801 ggacgatccg ggcaccaccg tcgacatcgc cgccaccgcg tccgaggacc ttcgcggcgt
     1861 ctggcgactg ctgcggggat tgaccagggg cgacataccg attgcggtgg tgcgtgagca
     1921 gttggcccgt ccggcactgc cggcgaccgt ggtgccgcag ggattgctcg gccaggtgct
     1981 tcggttcggg gcggtggggg tggcgtccac gctggcctac ttcgtgctct acctggtgct
     2041 gcggctcggt ctggacgccc aggtcgccaa cctgaccgcc ctggcgctca cggcggtggc
     2101 gaacaccgcc gccaaccggg tgttcacctt cggggtgcgt ggcaacgacg ggtggctcac
     2161 ccaccacgtc accggcctgg tcgcattcgc ggtcgcgctg gggttgacca gcgggtccct
     2221 ggccctgctg cacgggctgg cgcctgaggc cggacgcttc gtcgaggtga tggtgctggc
     2281 ggtggcgaac gcggtcgcga ccgccgtccg gttnnnnnnn nnnnnnnnnn nnnnnnnnnn
     2341 nnnnnnnnnn nnnnnnnnnn nnnnnnnnnn nnnnnnnnnn nnnnnnnnnn nnnnnnnnnn
     2401 nnnnnnnnnn nnntcgtcga ggtgatggtg ctggcggtgg cgaacgcggt cgcgaccgcc
     2461 gtccggtttc tggcgctcaa gttcgtgatg ggccgggccg gcgactgagc cggatccacg
     2521 ccgatctccg cttccctgcg gcacccggac gtgtgcgggg gcgtccgggc agatccgggg
     2581 ccgaggcacc cggacgtgcg cgggtcgtcc gggcgggccc ggggactcag gcacgcgggc
     2641 gtgtgcaggg gcgtccgggc gagaccccgg aactcaggca cccgaaggtg tgcggggtcg
     2701 tccgggcggg tccgaggacg gtctcaatcc ggaccgggac ggttcacaat ccggatccgg
     2761 gacgagttcc cgcgccggaa ccgggacgat tctcgaacgg cccgaaccgt cctgacggat
     2821 tgaggcgcca cccgccgccg tcctggaccg ggcgagcggc gcgcaccgta gactcccggc
     2881 gtggtgaatg tcggcgatgc cccggtggtg gcgcaggtgg tccgcaacgg gttcgtcgaa
     2941 tcgacccatc gcggcctggt ggtgatcacc cgcgcggacg ggtcggtgtc gtactcccgc
     3001 ggcgactaca acagccacat tctgccccga tcgtcgttga agccgattca ggcgctggcc
     3061 atggtgcgct gcggactcga cgtgccggcc gaccagctgg ccatcgcctg cgccagccac
     3121 tcgggcgaac cgatgcatct gcagaccgtg ctcgatttgc tgcacggcgc ggggctgagt
     3181 gagagcgacc tgcgcaacac ccccgacctg ccgttgatcg acgcgcaacg caacgagtgg
     3241 atcgcggccg gccgtccccc gagctcgctg gcgcagaact gttcgggcaa gcacgccgcg
     3301 atgctggcca cctgcaagct caacgactgg ccgaccggca cctatctcaa ccgcggccat
     3361 ccgtgccagc aggcggtcat cgaaaccacc cgcggcttcg gccagcacgt cttctggacc
     3421 tcgatcgacg ggtgcggagc acccgcctac gcgatctcgc tggtgggcct ggcccgcatc
     3481 ttcggcattc tcgcggccgc caccgagggc gccgagaagg ccgtcgccga cgcgatgcgt
     3541 gcccaccccg aactggtcgg cggcaccgga cgcgacgtca ccgacctgat gcgtggcgtg
     3601 cccggcatga tcgccaagga cggcgccgag ggcgtctacg ccgccggcct gcccgatgga
     3661 cgcggcatcg cgatcaagat cacggacggc ggggtgcggg cccgtccggt ggtgatgggg
     3721 gccgtcatgc gccggctgtg gctgggcgat gagtcgctgc gtcgccggct gatggacatg
     3781 ccgatctacg gccgcggggt gccggtcggc gcagtgatcc cgatcgacat ctgacccggg
     3841 ctgacgtgtc gggtcgggtc ggggggtcgg gtcgacattg cgcggcggtg cgttcgttgc
     3901 gcgggttcgt ccgcgcgcaa cgtgcgcgag accgcgcgac gtgccgagcg ctacttcgca
     3961 ccccgcgtct tcgggggatc ggccgggctg tggtgggaac cagcggacgg ggtggtcacc
     4021 accggcaccg cgtgcggtcc ggcgaagggc agcgccggct ttggtttggc ggccgacggc
     4081 ggtgtggccg aggtcgaggc ggtcgagccg gacgcggcgc cgaccgccgg tgcggaaccg
     4141 gtccccccgg ccgggttgac ggtgcctgac gcgccggcac cgtgcgtgcc cgcattcgac
     4201 gtgcccacgg ccggtgtcgc accggcgtcg gacgcaggcg tggtcgatgc cggctcggcc
     4261 gacgcacccg aaggcggcgc agccggctcc ggcgcgcgcc gtccgaacct ggaacctgca
     4321 tcagcacccg ctcgggacgg gaagccggcg gcctgtccga tgccccagat gtaggtcagg
     4381 gtcgccacga agggcagcag gtgcccgagc catccgaccc agatggccag atcggcattc
     4441 ggcgaggtgg agccggcgac gtaggccgtc cagccgaaca gcaccagcca gtacagcgcg
     4501 agcagcgtgg tgcccgtcca cagcagcggg cggccgcggc cgatccgtcg ggcgacatag
     4561 accgtggcgg cgaccgtggc caccacgaac gccaacgtgc gcaacaacga caggacgatt
     4621 tccatcagcg gcctcctcgg gggcggtagc gcagccaccc tagtcgcgga ccggtcgctc
     4681 acaccgcatc ccgttggggg tactgagtcc ggttgacccg gatcgtggct gggaagggtt
     4741 ttccgaatcg gttgcagggc cccaacggca ggtgttaggt agacgcatga gttcaaaggt
     4801 gaacgccacg accatgcagg tgaccagccc ggatgatgtg cgcaacgtcg tcctgatcgg
     4861 caacgcgggg tcgggcaaga ccactttgtt cgagcagctg ttgaaggctc gggtcgacgg
     4921 ttatcgaggc gaaaaagaag acgccgagcg ggcctcgcag ttgtttctgg cctcgttcgt
     4981 caccggtgac accgtggtca acctgctgga cgcccccggg caccccgact tcgtcggtga
     5041 gttgcgggcc ggcatccggg cggccgacgc ggcgatcttc gtactctcgg cagccgacgg
     5101 catcgacggc gccgcgcagg ctctgtggga ggaatgccgc gaggccaacc tgccgcgggt
     5161 gatcgtgctc accaagctgg acgccgggca cgcggacttc gacacctccg tcgaagagtg
     5221 ccaggcggtg ttcggcgagg gcgtccggcc gacccatgtg cccaccttct cgggccccaa
     5281 ccaggtctcg ggcaacatcg gcctgctcag cctcgaagaa cacgactact ccggcggcgc
     5341 gccggtcagc cgcaccctga acgcccacga cgaccaggtc gagacctacc gcggcccgct
     5401 gatcgagtcg atcattcaag aggccgagga cgacggcctg atggaccgct acctcgaaga
     5461 cgacgagcac ctcgacgtca acgacctgct cgacgatctg atgaaggcgg tggccacggc
     5521 gaacctgttt ccggtcgtcc cggtgcagac cggcaccggc gtcggtatcg aagagctgct
     5581 gcggctgatc gaacacggct tcccgacccc cggcctgcac ccgaatccgt cgctgaccac
     5641 caccgggggc gaggtcctgc cgcaaccgga caccgacccg tccgccccgc tggtggcaca
     5701 ggtgatccgc accaccaccg acccgttcgc cgggcggctg tccatggtgc gcatcttctc
     5761 cggcaccctg cacaccgacg acgtggtgca cgtgtcgggc catcggtcgt ggagcgcggg
     5821 gcagagcgac aatgcgcacc ccgatcacga caacgaggag cgcatcgggc tcattcaggg
     5881 cgccgtgggc acggagctga agccccgcca gtcggcgatg gcgggcgaga tcgtgctgct
     5941 gcccaagctg ggtaccgccg agaccggcga cacgctgtcg gcgaaggaac gtccgggtgt
     6001 ggtcgaggcc tggccgctgc ccgacccgct gctgccgctc gcgatccggg ccgccacccg
     6061 caacgacgag gacaagttgg ccggcgccct gcaacggctc gcggtcgagg actccagcgt
     6121 gcgtctggat cgcggggccg gcgaccaact cgtgttgtgg accaccggcc aggcccacgc
     6181 cgacctggtg cttggccggt tggtcgagcg ctaccaggtg cgggtcgagc aggaggatct
     6241 gaagatcccg atgcgcgaga ccttcaccgg caaggccgac gcgatggggc gtcacgtcaa
     6301 gcagtccggc ggccacggcc agtacgccgt gtgcaacatc gaggtcgagc cgctggagcg
     6361 cgggtcgggc ttcgagttcg tcgacaaggt ggtcggcggg gccgtcccgc gtcagttcat
     6421 tccctcggtc gagaagggca ttcgcaccca gctcgaacgc ggcgtgttcg ccggctaccc
     6481 gatggtcgac cttcgggtca ccctgtacga cggcaagtcg cactcggtcg attcgtccga
     6541 catggcgttc cagatggccg gtgcactggc gatcaaagag gcggcggcgg ccaagggcgg
     6601 tgtggtggcg ctgctcgagc cggtcgacct ggtcaccgtc acggtgggcg aggactactt
     6661 gggtgccgtg atgaccgacc tgtccgggcg gcgcggccag gtgctcggct cggactcggt
     6721 gaacagaaag gcgatcgtgc gcgctttggt gccgcaggcc gaactgaccc ggtacgcgat
     6781 cgacctgcgg ggcctcgggc acggctcggg caccttcacc cgcgagtttc acggctacga
     6841 gttgctgccg tccaacctgc tggacaagtt cgccaagaag tagccgcctg aaatccctgc
     6901 accagtgcgg gggctcccgc ctcggcgagc ggtgaccctt ggcactggtg ctgggttttc
     6961 aggcccccac cgcggtacgc accagctcaa tgaaggtgtc gggctcgtct gtcagcactt
     7021 gggcggcgaa gggcaacacg atccagtgcg cagcggccag ccgggcgtag cgccaccggt
     7081 ccttcacgaa cgcggaacgc gtgccgtgat gctcccagcc atcgatctcg accgccactc
     7141 gttgttgggg aaaggcgatg tcgacgaagt gcgtgatctc accgatgcgg atccggtagt
     7201 tcgtacgcca cccgccgatg cgggcggccc gaagcagttg gtgtgccatt cgctcgggct
     7261 cggaccaggg cgcgtcgcgc gagtcgtgca gcagcaggcg ccgccgggtg ttgcccgggc
     7321 ggtgcgggca cctatgcagg ccctgccaaa gctcgggcaa ggtggtgctg ccgcggcgca
     7381 gcgcctcatc gatcaccgtt ccaccgagtg tggggatcaa atcgagcacc gacagtgcgg
     7441 ggcagacgaa ccgcagatcg ccccggtgaa cgatgagctc gggaggaatc gaccgccgtt
     7501 cccacgagaa gcccgaatag gcccgttcaa cggtgccgct actcagcgcg gcagagacag
     7561 cggtgatcgg gcattcgggc caccacagag atctggcggc ggcagcaccg gtgatgaccg
     7621 catcgcaccg aaacgcagcg gccgcgcgca tgcgcgcctc ccacgaaggt tcaggttcgg
     7681 tgtagacgcc cggcagaatc gcccgcagtc tgccgcgacg tatgcactga tcgagttgac
     7741 gggtcagggt gcggtgccgc ttgcgttgca gcaccccacc ctcgaggtgc agcagttcca
     7801 aaattcgctc catacccttg aatgcacttt ttgaggcccc ttttgtgagc gcccgccccg
     7861 agtttgttgg cagcagcatc cggagggctc tcgcgccccg cctgaaatcc ctgcaccagt
     7921 gcgggtactc ccgcctcggc gagcggcgac ccctcgcact ggtgcagggt tttcagggcg
     7981 ggcggtcaga caggcaaaac ggcccggggg tgatcccccg ggccgtcgat caggtcgagc
     8041 tgtcagaacg agtacacggc gttggcgccg tagggcacct ggcggctgtc gacgatctct
     8101 ttgccgagcg gcacgcaggt gaccgggatc atcttgatgt tggcgatcgc cagcgggatg
     8161 ccgacgatcg tgatggcctg agcggcggcg gtggtcaggt ggccgatggc gagccagagc
     8221 ccggcgacca ggaaccagat gacgttgccg agcgccgacc cgacgccggc ggtcggcttg
     8281 ctgacgatcg tcttgccgaa cggccatagc gcgaacgacg ccatgcggaa cgacgcgatg
     8341 ccgaagggaa tggtgatgat cagaatgcag gccaggatgc cgaagatgaa gtagcccagg
     8401 gccagccaga agccgccgaa gaccagccag atgatgttga gcagagtacg catgagggtt
     8461 ttgattgcct ttcgcggggc gcgtcgtgcg ccgaccttct ccagtgtgcc gggtcgcatc
     8521 gccgcgggcc gggcagggca gggcagggtc agggacgagt ggggggtcag gcgctcgcca
     8581 tcatccggcc cgcacggatg ctgcgccgcc acccggacac cccgcaactc gccacctggc
     8641 gcgccgccac ccggacaccc cgcaactcgc cacctgcgcg ccgccccccg cgggcacccg
     8701 caactcgcca cctgcgcgcc gcccccacgc gggcacccgc gactcgccac ctgggcgccg
     8761 cacccggacc cccgccggcg gcaccccgca acggcctgac cccgcgtgcc cccttcgccg
     8821 acctctccac accgcgcggt cgaggtgcct cagggatgcg cagttgcccc cgcgtggtga
     8881 cgcggcccga cgtgcgcaat gaggagagcg cggagcagcc gctcacctgc gcgcggctac
     8941 cctgacccca tggacgaacc ccgtgcgcgc gtgcagcgcg aggtggtgtc gttcgtccgc
     9001 cgcagcgccc gcatgaacga aagccaactc aaggcctgga cgcatctgca gcattgggtg
     9061 gtctcggttc cgacgcgaca actctccacg tccatcgccc ccgaagcccg cgtcgactgg
     9121 cccgcggtgt tcggacgaga ggcgcccctg gtggtcgaac tgggaggcgg caccggcgac
     9181 gtgatcgcgg ccgtggccgc gcggcacccc gatcgagact tcatcgcctt cgaggtgttc
     9241 gaacccgcgg tggccagcac cctgtcgaaa tgtgcgcgtg ccggggtgac caacgtgcgg
     9301 gtggtggtcg ccaacggcgt ggacgggctc accaccctga tcgatgaggc cgcactcagc
     9361 gagctgtgga ccttctttcc cgacccctgg cacaaggcgc gccaccataa gcgccgcctg
     9421 gtctcgcccg atttcgccgc agtggccgcc cacactctgg ccccggacgg gctgtggcgg
     9481 ctggccaccg actgggagga ttacgcgctg gcgatccgtg cggtgctcga tgcgaacccg
     9541 cggctgcaga actgtcacga cggctgggcg ccgcggtggc aggatcgtcc gcagagccgg
     9601 tacgaggcca aagggctggc cgcgggccgc accatctacg acctgtgcta ccggcgaacg
     9661 cgagagagga gggacccgtg aggcttcgtc tcgacatcgc ctacgacggc accgagtttc
     9721 acggctgggc cggccagccc ggcctgcgca cggtccagga ggagctagaa gcatggattc
     9781 cgcgggtgct gcggcttgcc gaaccggtca ccctcacctg cgccggacgc accgacgccg
     9841 gcgtgcacgc ccgcggtcag gtcgcccatc tcgatctgcc cgagccggac gatgccgaac
     9901 gcaccgtcgc cgacctgacc tacaagctgc gcaacgtgct accgccggac gtggtggtgc
     9961 gccgggtcag cgtggccccg cccggcttcg acgcccggtt ctcggcgatc tggcgccgct
    10021 acgtgtaccg gatcgccgac gccacccccg acccgctgct gcgcatccac gtcacaccga
    10081 tgcgcaccga actcgacctc ggtcggatca acgcgctggc tccacgactg atcggactgc
    10141 acgacttcgc cgccttctgc aaggcccgcg agggtgcgac cacgatccgc aacctgcagc
    10201 acctgtcggc gcggcgcacc tggggcgacg tggccgagat caccgtgcgg gccgacgcgt
    10261 tctgtcactc gatggtgcgc tcactggtcg gcggattggt cgcggtggcc acggctcgac
    10321 gcgagcccga ctggctgatc ggtctactgg gcgcgtccga gcgggcgggt gagatcaagg
    10381 tgatgcccgc gtgtgggctg accctcgaag aggtcgggta tccgcccgat cgagagttgg
    10441 cggcgcgggc acgcgaggcg cgggccgtcc gaaccctgga gtcggagtga gccactactt
    10501 caccgccccc accggccccg agaagcggcg caccctcgac gtgcggttct gggacaccga
    10561 ctggcggttc accaccgcca acgaagtgtt cagcggcgac ggtctggatc tcggcaccgc
    10621 cgttctgctc cgcgaaagca caccgccggc cgacgcgacc cgactgctcg ac
//
LOCUS       DAOSUG010000028        10567 bp    DNA     linear   ENV 10-JUN-2024
DEFINITION  MAG TPA_asm: Micropruina sp. isolate AMR_MDS_2195
            NODE_3510_length_10567_cov_4.525970, whole genome shotgun sequence.
ACCESSION   DAOSUG010000028 DAOSUG010000000
VERSION     DAOSUG010000028.1
DBLINK      BioProject: PRJNA925477
            BioSample: SAMN32864262
            Sequence Read Archive: SRR4236673
KEYWORDS    WGS; ENV; Metagenome Assembled Genome; MAG; Third Party Data; TPA;
            TPA:assembly.
SOURCE      Micropruina sp. (wastewater metagenome)
  ORGANISM  Micropruina sp.
            Bacteria; Bacillati; Actinomycetota; Actinomycetes;
            Propionibacteriales; Nocardioidaceae; Micropruina.
REFERENCE   1  (bases 1 to 10567)
  AUTHORS   Abdulkadir,N., Saraiva,J.P., Zhang,J., Stolte,S., Gillor,O.,
            Harms,H. and Rocha,U.
  TITLE     Genome-centric analyses of 165 metagenomes show that mobile genetic
            elements are crucial for the transmission of antimicrobial
            resistance genes to pathogens in activated sludge and wastewater
  JOURNAL   Microbiol Spectr 12 (3), e0291823 (2024)
   PUBMED   38289113
REFERENCE   2  (bases 1 to 10567)
  AUTHORS   Abdulkadir,N.
  TITLE     Direct Submission
  JOURNAL   Submitted (24-MAR-2023) Environmental Microbiology, Helmholtz
            Center for Environmental Research -UFZ, Permoserstr 15, Leipzig
            04318, Germany
COMMENT     The annotation was added by the NCBI Prokaryotic Genome Annotation
            Pipeline (PGAP). Information about PGAP can be found here:
            https://www.ncbi.nlm.nih.gov/genome/annotation_prok/
            
            ##Genome-Assembly-Data-START##
            Assembly Method        :: SPAdes v. 3.11.1
            Genome Representation  :: Full
            Expected Final Version :: Yes
            Genome Coverage        :: Not Applicable
            Sequencing Technology  :: Illumina MiSeq
            ##Genome-Assembly-Data-END##
            
            ##Genome-Annotation-Data-START##
            Annotation Provider               :: NCBI
            Annotation Date                   :: 05/04/2023 04:57:10
            Annotation Pipeline               :: NCBI Prokaryotic Genome
                                                 Annotation Pipeline (PGAP)
            Annotation Method                 :: Best-placed reference protein
                                                 set; GeneMarkS-2+
            Annotation Software revision      :: 6.5
            Features Annotated                :: Gene; CDS; rRNA; tRNA; ncRNA
            Genes (total)                     :: 2,776
            CDSs (total)                      :: 2,750
            Genes (coding)                    :: 2,714
            CDSs (with protein)               :: 2,714
            Genes (RNA)                       :: 26
            tRNAs                             :: 24
            ncRNAs                            :: 2
            Pseudo Genes (total)              :: 36
            CDSs (without protein)            :: 36
            Pseudo Genes (ambiguous residues) :: 0 of 36
            Pseudo Genes (frameshifted)       :: 2 of 36
            Pseudo Genes (incomplete)         :: 32 of 36
            Pseudo Genes (internal stop)      :: 2 of 36
            ##Genome-Annotation-Data-END##
FEATURES             Location/Qualifiers
     source          1..10567
                     /organism="Micropruina sp."
                     /mol_type="genomic DNA"
                     /submitter_seqid="NODE_3510_length_10567_cov_4.525970"
                     /isolate="AMR_MDS_2195"
                     /isolation_source="Activated sludge of municipal
                     wastewater treatment plants"
                     /db_xref="taxon:2737536"
                     /environmental_sample
                     /geo_loc_name="Canada"
                     /lat_lon="49.25 N 123.1 W"
                     /collection_date="2015-06-16"
                     /metagenome_source="wastewater metagenome"
                     /note="metagenomic"
     gene            complement(<1..2170)
                     /locus_tag="PLF56_01800"
     CDS             complement(<1..2170)
                     /locus_tag="PLF56_01800"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_015071041.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="excinuclease ABC subunit UvrA"
                     /protein_id="HOQ52327.1"
                     /translation="MPQRTADSHDLIRVQGARENNLKNVSIELPKRRLTVFTGVSGSG
                     KSSLVFGTIAAESQRLINETYSAFLQGFMPNQNRPDVDLLDGLTTAIIVDQERMGANI
                     RSTVGTATDANALLRILFSRLGQPHIGSSQAFSFNVASISGAGAVTIEKGGTTTKERR
                     SFSITGGMCPRCEGTGAVTDFDLTALYDDSKSLNQGALTIPGYSMEGWYGRIYRSSGF
                     FDPDKPIRDFTERELDTLLRKEATRIKVEGINVTYEGLIPKIQKSFLAKDVDAMQPHI
                     RAFVERAVTFATCPQCDGTRLTAAARSSKVNGISIADACAMQISDLAQWVGGLDDASV
                     APLLTSLRHVLDSFVEIGLGYLSLARPAGTLSGGEAQRTKMIRHLGSSLTDVTYVFDE
                     PTIGLHPHDIQRMNDLLLQLRDKGNTVLVVEHKPETIAIADHVVDLGPGAGSAGGEVV
                     FEGTVEQLRTSDTLTGRHFDDRASVKPSVRTPAGALKVRGASTNNLQNVDVDIPLGVL
                     VVLTGVAGSGKSSLIQGSVAGRDGVVTIDQTGIKGSRRSNPATYTGLLDPIRKAFAKA
                     NGVKPALFSSNSEGACPNCKGAGVVYTDLAMMAGVATTCEVCEGKRFEASVLDYTLGG
                     RDISEVLAMPVSQARGFFGSGDARTPAAHAILERLDDVGLGYLSLGQPLTTLSGGERQ
                     RLKLATHMGDAGGVLILDEPTTGLHLADVEQLLALLDRLVD"
     gene            complement(2272..>2626)
                     /locus_tag="PLF56_01805"
     CDS             complement(2272..>2626)
                     /locus_tag="PLF56_01805"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_017933327.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=2
                     /transl_table=11
                     /product="hypothetical protein"
                     /protein_id="HOQ52328.1"
                     /translation="FFVWLGSSLDLRAIAANPTAIGLGVALGTAALLAHGLMALTRQP
                     LPIALTTAAQLGVPIGAAALGKTMGVLVDGEATAMLLGALITIAMVTLLSARVAALVH
                     DPSATSEPDPAANGR"
     assembly_gap    2627..2636
                     /estimated_length=10
                     /gap_type="within scaffold"
                     /linkage_evidence="paired-ends"
     gene            complement(<2637..3497)
                     /locus_tag="PLF56_01810"
     CDS             complement(<2637..3497)
                     /locus_tag="PLF56_01810"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_017933327.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_component: GO:0016020 - membrane [Evidence IEA];
                     GO_process: GO:0006812 - monoatomic cation transport
                     [Evidence IEA];
                     GO_process: GO:0055085 - transmembrane transport [Evidence
                     IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="cation:proton antiporter"
                     /protein_id="HOQ52329.1"
                     /translation="MSFALLALICGVALLGPIVSLNRLAHIPVVIGELLVGVVLGQSG
                     FGVIESSDVTLSFLAQIGFALVMFVAGTHVPLRQRAMLGGLRVGVLRALGVGVLAAPL
                     GLLIAHLFGTGHAALYAVLIASSSAGIVLPSLGGLEVNTPAGLQMLVQLAVADAACIV
                     ALPLVLDPAHAARAGLGAVAVLAVAAVFFLLLWWSQRSGLRGRVHQVSERRGLAMELR
                     VTLTLLFTLSAIAVGLGVSVMLAGFAMGLAMSAAGEPRRVANQTFALTEGFFAPIFFV
                     WLGASIDLRHL"
     gene            complement(3494..3892)
                     /locus_tag="PLF56_01815"
     CDS             complement(3494..3892)
                     /locus_tag="PLF56_01815"
                     /inference="COORDINATES: ab initio
                     prediction:GeneMarkS-2+"
                     /note="Derived by automated computational analysis using
                     gene prediction method: GeneMarkS-2+."
                     /codon_start=1
                     /transl_table=11
                     /product="hypothetical protein"
                     /protein_id="HOQ52330.1"
                     /translation="MAKPDDKHGQPVRWLPAPQPHDFPAAASYLSLIADAATVDQLIR
                     RLQAGTIEHVKAKDLLRAAQLRLLPADNAHVASDLAKIAAGEPLSPILLVRGDLRHGV
                     PLQIADGYHRVCACYLTDENSDVPAVVADP"
     gene            complement(4083..4547)
                     /locus_tag="PLF56_01820"
     CDS             complement(4083..4547)
                     /locus_tag="PLF56_01820"
                     /inference="COORDINATES: ab initio
                     prediction:GeneMarkS-2+"
                     /note="Derived by automated computational analysis using
                     gene prediction method: GeneMarkS-2+."
                     /codon_start=1
                     /transl_table=11
                     /product="hypothetical protein"
                     /protein_id="HOQ52331.1"
                     /translation="MYDFIDEAPSDRILLVAHGLLGALGPAAQQAGPVQWAGERQVRL
                     YQLPRPAFAAEADAGRGVHRPPHVAERLAELDDLETAFEEVAGRLAAGGRVIGLAVPG
                     KLVTGRSGRAWLERVRSIARDIAGLVELEVWTVTAGHAALYDPTVQPIRTAS"
     gene            complement(4985..5917)
                     /gene="cysK"
                     /locus_tag="PLF56_01825"
     CDS             complement(4985..5917)
                     /gene="cysK"
                     /locus_tag="PLF56_01825"
                     /EC_number="2.5.1.47"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_016668424.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_function: GO:0004124 - cysteine synthase activity
                     [Evidence IEA];
                     GO_process: GO:0006535 - cysteine biosynthetic process
                     from serine [Evidence IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="cysteine synthase A"
                     /protein_id="HOQ52332.1"
                     /translation="MAYFKDATELIGRTPLVQLNRLYGDSEAIVLAKLEYYNPANSVK
                     DRIGVAIIDAAEADGSLQPGGTIVEGTSGNTGIALAWVGAARGYRVIITLPETFSKER
                     RAVLRALGAELVLTPGADGVPGANRRAAEIAAEIPGAILARQFDNAANVEVHRRTTAE
                     EIWADTEGTVDIVVSGVGTGGTITGVGQVLKQRKPGVQLYAVESAESAVLSGEPKGPH
                     KIQGISPGFVPSILDRDAFDGIIKVTSDDAIDWAKRVAKQEGLLLGISSGAALRAAAD
                     LAADPANAGKTIVVVAPDWGERYLSTPLYADLLD"
     gene            complement(5971..6411)
                     /locus_tag="PLF56_01830"
     CDS             complement(5971..6411)
                     /locus_tag="PLF56_01830"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_013159990.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="thioredoxin family protein"
                     /protein_id="HOQ52333.1"
                     /translation="MTGVWIVLAALTVALAFGGYRALTDGRARAVGASTSGDDTPRLD
                     AAQLGAPLGSRATFVQFSSSVCAPCRATHTLLAELTGERPSVAHIDLDAERRLDLVAQ
                     FGITRTPTVLLLDGSGAVRHRIVGAARRADVLDALDSVAGAQAA"
     gene            complement(6408..6860)
                     /locus_tag="PLF56_01835"
     CDS             complement(6408..6860)
                     /locus_tag="PLF56_01835"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_020392908.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="DUF4395 domain-containing protein"
                     /protein_id="HOQ52334.1"
                     /translation="MSSTAQTVAKHPLIDPRGQRFTAAITAGLLALTLILGPGAGLWI
                     LVVQTLAFAAGSLLGLSYSPWGLIFRSAVRPRLAPPTELEDARPPRFAQTVGLAFAVV
                     GLIGAIAGLSVLFYVAIGFALVAALLNAIFNFCLGCEIYLLGTRVLAR"
     gene            complement(7133..7372)
                     /locus_tag="PLF56_01840"
     CDS             complement(7133..7372)
                     /locus_tag="PLF56_01840"
                     /inference="COORDINATES: ab initio
                     prediction:GeneMarkS-2+"
                     /note="Derived by automated computational analysis using
                     gene prediction method: GeneMarkS-2+."
                     /codon_start=1
                     /transl_table=11
                     /product="hypothetical protein"
                     /protein_id="HOQ52335.1"
                     /translation="MPAHSGQPLVGEVLDLVGELKAAGSTIVMATHELGLARQAADRV
                     VFLADGVVVEQGSPAQVLDAPRQAATREFLSVRDR"
     gene            7397..8380
                     /locus_tag="PLF56_01845"
     CDS             7397..8380
                     /locus_tag="PLF56_01845"
                     /inference="COORDINATES: protein motif:HMM:TIGR00367.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_component: GO:0016020 - membrane [Evidence IEA];
                     GO_process: GO:0098662 - inorganic cation transmembrane
                     transport [Evidence IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="calcium/sodium antiporter"
                     /protein_id="HOQ52336.1"
                     /translation="MLALLVIGGLAALIAGAELVVRYGSLLARSMGVPPMIVGLTIVS
                     IGTSAPELAVGIDAMRVNAGSLAVGNIAGTNIVNLLLILGLSAAIRAIDFDRRTLVLD
                     LPAMAVAAVLVFGLSFDGQLQQWEGIPLVLVAVAYTVLLGWWTRREHLSTSDEISADL
                     AVEARITAPAEYRRRRLVWFLLLLLAGIAVIVVGADWLVRGSVELARVFGVSDAFIGL
                     TVVAIGTSAPELVTALVSTLRGDRDIAVGNLIGSSVYNLTLILGVPVLVANGSGPIEN
                     YLLHIDLPLMVGVCLLCVPLFLTGRRLSRAEGACMVAGYALYLGYLLIARS"
     gene            complement(8412..9497)
                     /locus_tag="PLF56_01850"
     CDS             complement(8412..9497)
                     /locus_tag="PLF56_01850"
                     /inference="COORDINATES: protein motif:HMM:NF012318.2"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="trypsin-like peptidase domain-containing
                     protein"
                     /protein_id="HOQ52337.1"
                     /translation="MTMSAPGGARSAGLRLAVAGLALGLIGGLVGAFTATRLTSVPAP
                     GAAAGGSCDSVQVAQSVLPAVVTVFATGADGQGGSGSGAVTTSDGVIVTNDHVIHVAG
                     TKGTIEVLLNSGERLPATLVGTDPITDLAVLRVARTGLPTLPIDAANSVVVGQPVVAL
                     GAPLGLSGTVTSGIISAVGRNVPVPTGAGGTTILTGALQTDAAINPGNSGGPLVTCDG
                     RLIGVNTAGATVPDAGGVAGGGSVGINFAVPSSTASRITSEILASGRATHPWIGAQTA
                     DITPALANRYGAKAGLFVQAVATDGPAARAGLRRGDVITTIAGQPASSLSLAWLLVSA
                     KVGDQVEVEFVRGGAAQRATLTLIEQP"
     gene            10324..>10567
                     /locus_tag="PLF56_01855"
     CDS             10324..>10567
                     /locus_tag="PLF56_01855"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_002546559.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="nitrate reductase subunit alpha"
                     /protein_id="HOQ52338.1"
                     /translation="MTSTRAHLDGPAADVLLKLGRYFTKWDETADGRAVFREGGRQGD
                     VFYRDRWSHDKVVRSTHGVNCTGSCSWKVYVKDGIIT"
BASE COUNT         1691 a         3656 c         3643 g         1567 t
ORIGIN      
        1 cgtcgaccag ccggtcgagc agcgcgagca gttgctcgac gtcggccagg tgcaggccgg
       61 tggtcggctc gtccagaatc agcacgccgc ccgcatcgcc catgtgcgtg gccagcttga
      121 gccgctgccg ctcaccgccc gagagcgtgg tcagcggctg gccgaggctc aggtagccca
      181 ggccgacatc gtccagccgt tcgagaatgg catgggcggc gggcgtccgg gcgtcccccg
      241 agccgaagaa cccccgcgcc tgcgacaccg gcatcgcgag cacctcgctg atgtccctgc
      301 cgccgagcgt gtagtccagc acggacgcct cgaaccgctt gccctcgcac acctcgcagg
      361 tggtggccac gcccgccatc atcgccaggt cggtgtacac cacgccggcg cccttgcagt
      421 tcgggcaggc accctcggag ttcgaactga acagcgccgg cttcaccccg ttggccttgg
      481 cgaacgcttt gcggatcggg tccagcagac cggtgtaggt cgccgggttg ctgcgccgcg
      541 agcccttgat gccggtctgg tcgatggtga ccacgccgtc gcgtccggcc acggaaccct
      601 gaatcagcga actcttgccc gaaccggcca ccccggtcag caccaccagc accccgagtg
      661 gaatgtccac gtcgacgttc tgcaggttgt tcgtggacgc cccgcgaacc ttcagcgcgc
      721 cggcgggcgt gcgcaccgac ggcttcaccg acgcccggtc gtcgaagtgc cgtccggtca
      781 gggtgtcgct ggtgcgcaac tgctcaacgg tgccctcgaa caccacctcg ccgccggccg
      841 agccggcgcc cgggccgagg tcgacgacgt ggtcggcgat cgctatcgtc tcgggcttgt
      901 gctcgaccac cagcaccgtg ttgcccttgt cgcgcagctg cagcagcagg tcgttcatgc
      961 gctggatgtc gtgcgggtgc agcccgatgg tcggttcgtc gaacacgtag gtgacgtcgg
     1021 tcagcgacga gccgaggtgc cggatcatct tggtgcgctg cgcctcgccg cccgacagcg
     1081 tgccggccgg acgggccagg ctcaggtagc ccagaccgat ctcgacgaat gagtcgagca
     1141 cgtgccgcag cgaggtcagc agcggcgcga ccgacgcatc gtccagcccc ccgacccact
     1201 gcgccaggtc gctgatctgc atggcgcagg cgtccgcgat gctgatgccg ttcaccttgg
     1261 acgaccgcgc cgccgcggtc agccgggtgc cgtcgcactg ggggcaggtg gcgaaggtga
     1321 ccgcccgctc gacgaaggcg cgaatgtgcg gctgcatggc gtccacgtcc ttggccagga
     1381 acgacttctg gatcttcggg atcaggccct cgtaggtgac gttgatgccc tcgaccttga
     1441 tgcgggtcgc ctccttgcgc agcagggtgt cgagctcgcg ctcggtgaag tcgcggatcg
     1501 gcttgtcggg atcgaagaac ccgctgctgc ggtagatgcg gccgtaccag ccctccatgc
     1561 tgtagccggg aatggtcagc gcgccctggt tgagcgactt ggaatcgtcg tagagcgcgg
     1621 tcaggtcgaa gtcggtgacc gcgccggtgc cctcgcagcg cggacacatg ccgccggtga
     1681 tgctgaagct gcgccgctcc ttggtggtgg tgccgccctt ctcgatggtg accgccccgg
     1741 cgccgctgat cgaggccacg ttgaacgaga acgcctggga cgacccgatg tgcggctggc
     1801 cgagtcggct gaacaggatt ctgagcaggg cgttcgcgtc cgtcgccgtg cccaccgtgg
     1861 agcggatgtt ggcccccatc cgttcctggt cgacgatgat cgcggtggtc aggccgtcca
     1921 gcaggtcgac gtccggacgg ttctggttcg gcatgaaccc ctgcagaaag gcgctgtagg
     1981 tctcgttgat cagccgctgg ctctcggcgg cgatggtgcc gaacaccagc gagctcttgc
     2041 ccgaccccga aaccccggtg aacaccgtca gccggcgttt gggcagctcg atactgacgt
     2101 tcttcaggtt gttctcgcgg gcaccctgga cccggatcaa gtcatggctg tcggcggttc
     2161 gctgcggcat cgcggccctc tctgcacggc cgcccgtcgc aggccgtggg cagccaccct
     2221 aacgcgcccg tccgggcgct gccccctgga tcaggtcagg gctaccgggg gctaccggcc
     2281 gttagctgcc ggatccggct cgctggtcgc ggacgggtcg tgcaccagcg ccgcgacccg
     2341 cgcgctgagc agcgtcacca tcgcgatggt gatcagggca cccagcagca tggctgtcgc
     2401 ctcgccgtcc acgagcacac ccatggtctt gcccagtgcc gcggcaccga tcggcacgcc
     2461 cagctgggcg gcggtggtca aagcgatcgg cagcggctgc cgggtcagcg ccatcagccc
     2521 gtgggcgagc agcgcggccg tcccgagcgc cacgcccaag ccgatggcgg tcgggttggc
     2581 cgcgatcgcc cgcagatcga gcgaactgcc gagccagacg aagaagnnnn nnnnnngagg
     2641 tggcgcaggt cgatggaggc tcccagccag acgaagaaga tcggggcgaa gaacccctcg
     2701 gtcagggcga aggtctggtt cgccacgcgg cgcggctcgc ccgcggccga catcgccagc
     2761 cccatcgcga acccggccag catcaccgag accccgagac ccacagcgat cgccgagagc
     2821 gtgaacaaca gggtcagggt gacccgcagc tccatggcga gaccgcgccg ctcactcacc
     2881 tgatggacgc gcccgcgcag cccgctgcgc tgcgaccacc acagcagcag aaagaacacc
     2941 gcagccacag ccaggaccgc aacggcaccg agcccggccc gggccgcatg cgcggggtcg
     3001 agcaccagcg gaagcgcgac gatgcaggcg gcgtccgcga cggccagttg caccagcatc
     3061 tgcagcccgg caggggtatt cacctcgagg ccacccaggc tgggcaacac gatgccggcg
     3121 gacgacgacg cgatcagcac cgcatacaag gctgcgtgac cggtgccgaa caggtgcgcg
     3181 atcagcaggc cgagcggtgc ggccagcacc ccgacgccga gcgctcgcag tactcccacg
     3241 cgcaggccgc cgagcatggc ccgctgccgc aacggcacat gggtgccggc cacgaacatc
     3301 accagagcga agccgatctg cgccagaaac gacagcgtca catcgctcga ttcgatcacc
     3361 ccgaagcccg attgacccag caccaccccc accagcagct caccgatcac caccggaatg
     3421 tgcgccagtc ggttcaggct gacgatcggc cccagcagcg ccacgccgca gatcagggcg
     3481 agcagcgcga aactcacgga tcggccacga cggcgggcac gtcactgttc tcgtcggtga
     3541 ggtagcaggc acagacccgg tggtagccgt ccgcgatctg cagggggacg ccgtggcgca
     3601 ggtcgccccg aaccagcagg atcggcgaca gcggctcacc ggccgcgatc ttggccaggt
     3661 cggaggcgac gtgcgcgttg tcggccggca gcaggcgcaa ctgggcggcc cgcagcaggt
     3721 ctttggcctt gacgtgctcg atcgtgccgg cctgcaaccg ccggatcagc tgatcgaccg
     3781 tggctgcatc ggcgatcagg ctcaaatacg acgcggccgc cggaaagtca tgcggctgcg
     3841 gagcgggaag ccaacgcacc ggttgaccgt gcttgtcatc gggtttcgcc atctcgacct
     3901 ttcgcctggg caactgtagg ccgtccgaac gaagtctcac cccgggtccg cgcgccagcc
     3961 gcgagatcgg cggcggcgag catcacctgc tcgaccaggt gcccgcgacc gcgggcaacc
     4021 tcacgcggcg atcacgggcg accggtcagc ggtcgcgacg ctcgcgggcg gccggtcggc
     4081 ggtcaggacg cggttcggat cggctgcacg gtcgggtcgt agagggcggc gtgcccggcg
     4141 gtcaccgtcc aaacctccaa ctcgaccagg ccggcgatgt cgcgagcgat cgaacgcacc
     4201 cgctcgagcc aggcccgtcc ggagcgaccg gtcacgagct tgcccggaac ggccaggccg
     4261 atcacccggc cgcccgcggc gagccgaccc gccacctctt cgaacgcggt ctcgaggtca
     4321 tcgagttcgg cgagccgctc ggccacatgc ggcggccgat gcacgccccg acccgcgtcg
     4381 gcttcggcgg caaaggcagg ccgcggcaac tggtacagcc gcacctgacg ctcacccgcc
     4441 cactgcaccg ggccggcttg ttgagcggcc gggccgaggg caccgagcag gccgtgggcg
     4501 actagaagga tgcgatcact gggtgcttca tcgatgaagt cgtacatgaa gtcaagcttt
     4561 ctgatgtggg gtgatgctgc gggtcagctg ctttcgagtg cgatgacttg aaagcgggat
     4621 ccgaggtcaa gagccgggcc ggtcatgaaa gatacgcgat gctcggtgca tctcggatgc
     4681 aggtgggtgg cggacaatca gctgtgtgtc aacgccaaca catgcgtacc caacaacaca
     4741 cgttcacagt agtgaggacc cccgaacgca tggcaatcga tggcattgag accccctcag
     4801 tgaacatggt tcgaatctaa aacagaatgc ccgggatcac aagcgagaaa tgtaaacatc
     4861 tcatgaacat gatttgtgtc ggatgcgcgc atattacggg ttacgcatgg cattaacccg
     4921 caccgtgtcc cgcgagcacc ggtcatacga atcgaccggc gtcgcgggcg cggtgccgaa
     4981 cgggtcagtc gagcaggtcg gcgtacagcg gggtgctcag gtagcgctcg ccccagtcgg
     5041 gtgccaccac gacgatggtt ttgccggcgt tggcggggtc ggcggccagg tcggcggccg
     5101 cccgcaatgc cgcgccggag ctgatgccca acagcaggcc ttcctgcttg gccacccgct
     5161 tggcccagtc gatcgcgtcg tccgaggtga ccttgatgat gccgtcgaac gcgtcacgat
     5221 cgagaatcga gggcacgaag cccgggctga tgccctggat cttgtgcgga cccttgggct
     5281 cgcccgacag caccgccgac tcggcggatt cgacggcgta caactgcacg cccggcttgc
     5341 gttgtttgag cacctggccg acgccggtga tggtgccccc ggtgccgaca cccgagacca
     5401 cgatgtcgac cgtgccctcg gtgtcggccc agatctcttc ggccgtggtg cggcgatgca
     5461 cctcgacatt cgccgcattg tcgaactgac gagccagaat ggcacccggg atctcggcgg
     5521 cgatctcggc cgcccgccgg ttcgccccgg gcaccccgtc cgcgccgggg gtgagcacca
     5581 gttcggcacc caacgcgcgc agcaccgcgc gacgctcctt gctgaaggtc tcgggcaggg
     5641 tgatgatcac ccggtagccg cgggccgcgc cgacccaggc cagcgcgatg ccggtgttgc
     5701 cggacgtgcc ttcgacgatc gtgccgcccg gctgcagcga accgtccgcc tcggcggcgt
     5761 cgatgatcgc cacaccgatc cggtccttca ccgagttggc cgggttgtag tactcgagct
     5821 tggcgagcac gatcgcctcg ctgtcgccgt agagccggtt gagttggacc agcggggtgc
     5881 gtccgatcag ctcggtcgca tctttgaagt aggccatggt tgtgtgttcc tcccgtgatg
     5941 aagtgaatcc agattggtaa tgcgttgcgc tcaggcggcc tgggcccccg ccacagagtc
     6001 cagcgcgtcg agcacgtccg cacgacgggc agccccgacg atgcggtgcc gcaccgctcc
     6061 gctgccgtcc agcagcagca cggtgggcgt gcgagtgatg ccgaactgtg cgacgagatc
     6121 gagccggcgc tcggcgtcca ggtcgatgtg cgcaaccgac ggccgctcgc cggtcagttc
     6181 cgccagcagc gtgtgggtgg cccgacacgg cgcacagacg ctggacgaga actgcacgaa
     6241 cgtggcgcgg ctgcccaggg gtgcgcccag ctgggctgcg tccaggcggg gggtgtcgtc
     6301 gccgctggtg gacgccccca cggcgcgggc ccgtccgtcg gtcagggcgc ggtagcctcc
     6361 gaacgcgagc gcgacggtca gcgcggcgag cacgatccag accccggtca tcgggccagc
     6421 acccgcgtgc cgagcaggta gatctcgcaa cccaggcaga agttgaagat cgcgttcagc
     6481 agggccgcca cgagcgcgaa cccgatcgcc acgtagaaca acacgctcag gcccgcgatc
     6541 gcgccgatca ggcccaccac cgcaaaggcc aggccgacgg tctgcgcgaa ccggggcgga
     6601 cgggcgtcct cgagttcggt gggcggggcc agccgcggac ggaccgcgct gcggaagatc
     6661 agcccccacg ggctgtacga cagacccagc agtgacccgg cggcgaacgc cagggtctgc
     6721 acgaccagga tccacaaccc cgcgccgggg ccgagaatca gggtcagcgc cagcaggccg
     6781 gcggtgatcg cggccgtgaa ccgctggccg cgtggatcga tgagtgggtg cttggccacg
     6841 gtctgagcgg tggatgacat cggaggtcct tcgaggcagt tcgttggtgg agttctgcct
     6901 gacaccgctc tgcgagagcc gcagttgcgt tgggggtgac cgcagcgagg gcgcgatcgg
     6961 aagaggcggt cgtcaggccg tcagcggcag cgacaacaca ttcgcacgca gacgcgcgcc
     7021 acgagtggtg cgaggcgtcc ggcgaacatg aagacaatgg tatcaccaac aaaggaggca
     7081 gcaggacgtg acgggtgtcg ccgggggaat gggcggctgg gtcgatgccg ggtcaccgat
     7141 cgcgaacgga caggaactcg cgggtcgcgg cctgccgcgg agcgtccagc acctgcgcgg
     7201 gcgaaccctg ctcgaccacg acgccgtccg ccagaaagac cacccggtcg gcggcctgcc
     7261 gggctaagcc gagttcgtgg gtggccatca cgatggtcga tccggcggcc ttgagctcgc
     7321 cgaccaggtc gagcacctcg ccgactaggg gttggccgga gtgagccggc acgtcgagct
     7381 ttcgctaacc tgagccatgc tcgcgctcct ggtgatcggt ggcctcgcgg ccctgatcgc
     7441 gggcgcggaa ctcgtggtgc gctacggctc gctgctggcg cgcagcatgg gcgtgccgcc
     7501 gatgatcgtc gggctcacca tcgtgtcgat cggcaccagc gcacccgaac tggcggtcgg
     7561 catcgacgcc atgcgggtca acgcggggtc gctggcggtc ggcaacatcg ccggcaccaa
     7621 catcgtcaac ctgctgctga tcctcggttt gagcgcggcg atccgggcga tcgatttcga
     7681 ccgccgcacg ctggtgctcg atctacccgc gatggcggtg gctgcggtgc tggtgttcgg
     7741 gctcagcttc gacggccaac tgcagcagtg ggagggcatc cccctggtgc tggtcgccgt
     7801 ggcctacacg gtgctgctgg gttggtggac gcgtcgcgaa cacctcagca cctcggacga
     7861 gatctctgct gatctcgccg tcgaggcccg catcaccgcg ccggccgagt accgacgccg
     7921 ccggctggtg tggtttctgc tcctgctgct ggccggcatc gccgtcatcg tggtgggcgc
     7981 cgactggctg gtgcggggat cggtcgaact ggcccgcgta ttcggggtct cggacgcctt
     8041 catcggtctc accgtggtgg ccatcggaac ctcggcgccc gaactcgtga ccgctctggt
     8101 gtcgaccctg cggggcgatc gtgacatcgc ggtcggcaac ctgatcggct ccagcgtcta
     8161 caacctgacg ctgattctcg gggtgccggt gctggtcgcg aacggatcgg gcccgatcga
     8221 gaactacctg ttgcacatcg acctgccgct gatggtgggc gtctgcctgc tgtgcgtgcc
     8281 cctgttcctg accggacgcc gactctcgcg cgctgagggt gcatgcatgg tggccggtta
     8341 cgccctctat ctcggctacc tgctgatcgc ccgcagttga gtagccaccc cggatcggcc
     8401 cagaagacgg atcagggctg ctcgatcagc gtcagggtgg cccgctgagc agcgccgccg
     8461 cgcacaaact cgacctcgac ctggtcaccg accttcgcgc tgaccagaag ccacgccaac
     8521 gaaaggctgc tggccggctg ccccgcgatg gtggtgatca cgtcaccgcg ccgcaggccc
     8581 gccctggcgg cgggaccgtc cgtggcgacc gcctgcacga acagcccggc cttcgccccg
     8641 taccgattgg ccagggccgg ggtgatgtcg gcggtctggg caccgatcca agggtgcgtg
     8701 gcgcgtccgc tggcaagaat ctcggacgtg atgcggctcg cggtcgacga cggcactgcg
     8761 aagttgatgc cgacgctgcc tccgccggcg acgccgccgg catcgggcac cgtcgccccg
     8821 gcggtattga ccccgatcag ccggccgtcg caggtcacca aaggtccgcc ggagttgccc
     8881 gggttgatgg ccgcgtcggt ctgcagggca ccggtgagga tcgtggtgcc gcctgcgcca
     8941 gtgggcaccg gcacgttgcg gccgacagca ctgatgatgc cgctggtcac tgtgcccgac
     9001 aggcccaggg gcgcaccgag tgccaccacg ggctggccca ccaccacgga gttggcggcg
     9061 tcgatcggca gcgtgggcag ccccgtgcgg gcaacgcgca gaaccgcgag gtcggtgatc
     9121 gggtccgtgc ccaccagagt ggccggcagc cgttcaccgc tgttcagcag cacctcgatg
     9181 gtgcccttgg tgccggcgac gtgaatcacg tgatcgttgg tcacgatcac gccgtcgctg
     9241 gttgtgaccg cgccactacc gctgccgccc tggccgtcgg cgccggtcgc gaacacggta
     9301 acgaccgcgg gcagcacgct ctgcgcgacc tgcacggagt cgcacgagcc cccagcggcg
     9361 gcaccgggtg cgggtaccga ggtgaggcgg gtcgccgtga acgcccccac caacccaccg
     9421 atcaatccga gcgcgagtcc tgcgaccgcc aaccgcagcc cggcggaacg ggcaccaccg
     9481 ggggcactca tggtcatgcc tgaacaatag ttctcccggg gcagcgcgcg gccgcgctga
     9541 cgtcggtggc gtccggcacg cttgtggcgc gtcgcagggt tcgtccagcg gcctccctcg
     9601 cgcgaaactg caccacattc tgggcccagc cacgctcaca cggcgtccgc gcccccaacg
     9661 gtggtgcagt ttcgcgacgc caaccgactc aaagcggccc gggacgagga tggctgcgtc
     9721 cgggacggga gtgagcggcg ccctggacgc acatcgctgc gaccgccgga gcacggagta
     9781 ggcggcgccg gtcgtcccgt cgcaggtgcg tccagcggcc tccctcgcgc gaaactgcac
     9841 cacattcgga gcccagccac gctcacacgg cggccgcagc cccaacgatg gtgcagtttc
     9901 gccaccgcca cccgactcgc gcagcccggg acgaggatgg cgccggccca gcccgcggcc
     9961 gcccgcacgg agtggccgcc ctcgccgggg gcgaggacgg gctgcatgcg ggacgcgcgt
    10021 cgctgcggcc gccgggcacg ggagtaggcg gcgccggtcg tcccgtcaca gggtgcgtcc
    10081 agcagcctcc ctcgcgcgaa actgcaccac attctgggcc cagccacgct cacacggcgt
    10141 ccgcgccccc aacggtggtg cagtttcacc accgccaccc gattcgcgcg gcacccgaag
    10201 gggtgaggcg acgcccggtc tcgccgcctg cgcaggctgg gtgcgcatcc tcaggcaagg
    10261 gaatatgcca acctttttgt aaaaaaaact gatctgcggg cgctgcgctg atatgcacta
    10321 ctcatgacct ccacgcgcgc acatctggac ggacccgctg ccgacgtcct gctcaagctg
    10381 ggccgctact tcaccaagtg ggatgagacg gccgatggtc gcgcggtgtt ccgcgagggc
    10441 ggccggcagg gcgatgtgtt ctaccgcgat cgctggagcc acgacaaggt ggtgcgctcc
    10501 acccacgggg tcaactgcac cggctcgtgc tcgtggaagg tgtacgtcaa ggacggcatc
    10561 atcacct
//
LOCUS       DAOSUG010000029        10502 bp    DNA     linear   ENV 10-JUN-2024
DEFINITION  MAG TPA_asm: Micropruina sp. isolate AMR_MDS_2195
            NODE_3534_length_10502_cov_5.056188, whole genome shotgun sequence.
ACCESSION   DAOSUG010000029 DAOSUG010000000
VERSION     DAOSUG010000029.1
DBLINK      BioProject: PRJNA925477
            BioSample: SAMN32864262
            Sequence Read Archive: SRR4236673
KEYWORDS    WGS; ENV; Metagenome Assembled Genome; MAG; Third Party Data; TPA;
            TPA:assembly.
SOURCE      Micropruina sp. (wastewater metagenome)
  ORGANISM  Micropruina sp.
            Bacteria; Bacillati; Actinomycetota; Actinomycetes;
            Propionibacteriales; Nocardioidaceae; Micropruina.
REFERENCE   1  (bases 1 to 10502)
  AUTHORS   Abdulkadir,N., Saraiva,J.P., Zhang,J., Stolte,S., Gillor,O.,
            Harms,H. and Rocha,U.
  TITLE     Genome-centric analyses of 165 metagenomes show that mobile genetic
            elements are crucial for the transmission of antimicrobial
            resistance genes to pathogens in activated sludge and wastewater
  JOURNAL   Microbiol Spectr 12 (3), e0291823 (2024)
   PUBMED   38289113
REFERENCE   2  (bases 1 to 10502)
  AUTHORS   Abdulkadir,N.
  TITLE     Direct Submission
  JOURNAL   Submitted (24-MAR-2023) Environmental Microbiology, Helmholtz
            Center for Environmental Research -UFZ, Permoserstr 15, Leipzig
            04318, Germany
COMMENT     The annotation was added by the NCBI Prokaryotic Genome Annotation
            Pipeline (PGAP). Information about PGAP can be found here:
            https://www.ncbi.nlm.nih.gov/genome/annotation_prok/
            
            ##Genome-Assembly-Data-START##
            Assembly Method        :: SPAdes v. 3.11.1
            Genome Representation  :: Full
            Expected Final Version :: Yes
            Genome Coverage        :: Not Applicable
            Sequencing Technology  :: Illumina MiSeq
            ##Genome-Assembly-Data-END##
            
            ##Genome-Annotation-Data-START##
            Annotation Provider               :: NCBI
            Annotation Date                   :: 05/04/2023 04:57:10
            Annotation Pipeline               :: NCBI Prokaryotic Genome
                                                 Annotation Pipeline (PGAP)
            Annotation Method                 :: Best-placed reference protein
                                                 set; GeneMarkS-2+
            Annotation Software revision      :: 6.5
            Features Annotated                :: Gene; CDS; rRNA; tRNA; ncRNA
            Genes (total)                     :: 2,776
            CDSs (total)                      :: 2,750
            Genes (coding)                    :: 2,714
            CDSs (with protein)               :: 2,714
            Genes (RNA)                       :: 26
            tRNAs                             :: 24
            ncRNAs                            :: 2
            Pseudo Genes (total)              :: 36
            CDSs (without protein)            :: 36
            Pseudo Genes (ambiguous residues) :: 0 of 36
            Pseudo Genes (frameshifted)       :: 2 of 36
            Pseudo Genes (incomplete)         :: 32 of 36
            Pseudo Genes (internal stop)      :: 2 of 36
            ##Genome-Annotation-Data-END##
FEATURES             Location/Qualifiers
     source          1..10502
                     /organism="Micropruina sp."
                     /mol_type="genomic DNA"
                     /submitter_seqid="NODE_3534_length_10502_cov_5.056188"
                     /isolate="AMR_MDS_2195"
                     /isolation_source="Activated sludge of municipal
                     wastewater treatment plants"
                     /db_xref="taxon:2737536"
                     /environmental_sample
                     /geo_loc_name="Canada"
                     /lat_lon="49.25 N 123.1 W"
                     /collection_date="2015-06-16"
                     /metagenome_source="wastewater metagenome"
                     /note="metagenomic"
     gene            <1..1210
                     /gene="ppk1"
                     /locus_tag="PLF56_01860"
     CDS             <1..1210
                     /gene="ppk1"
                     /locus_tag="PLF56_01860"
                     /EC_number="2.7.4.1"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_013865608.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_component: GO:0009358 - polyphosphate kinase complex
                     [Evidence IEA];
                     GO_function: GO:0008976 - polyphosphate kinase activity
                     [Evidence IEA];
                     GO_process: GO:0006799 - polyphosphate biosynthetic
                     process [Evidence IEA]"
                     /codon_start=2
                     /transl_table=11
                     /product="polyphosphate kinase 1"
                     /protein_id="HOQ52339.1"
                     /translation="PLDLRGLFSLADLDREALKYPAFLPRTHPELAEVETAKPADMFA
                     ALKQRDVLLHHPYHSFATSVQRFVEQAAADPAVLAIKQTLYRTSGDSPIVDALIDAAQ
                     AGKQVLALVEIKARFDEQANIAWARKLERAGCHVVYGLIGLKTHCKLSLVVRDEPDGL
                     RRYCHIGTGNYNPKTARMYEDVGLLTANPVVTDDVARLFNHLSGMGQETHYKRLLVAP
                     HGIRRGLIEFIEDEIAHRRAGRPAGIRIKVNSIVDEAVIDALYRASQAGVPVDLWVRG
                     ICTVKPGVPGLSDNIRVRSILGRFLEHSRLFWFANDGQPRVGLGSTDLMHRNLDRRVE
                     VLVSLTNPRHIAQIEEWFDLAFDEGTASWWLDQNGWTQRTADAEGEPLLDSQEHLIQL
                     VSRRPDPRVV"
     gene            1210..2094
                     /locus_tag="PLF56_01865"
     CDS             1210..2094
                     /locus_tag="PLF56_01865"
                     /inference="COORDINATES: protein motif:HMM:NF012514.2"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="NUDIX hydrolase"
                     /protein_id="HOQ52340.1"
                     /translation="MAGSRLVEAAGAVVLRRAKGRQVQVLLIHRPSYNDWSLPKGKLE
                     TDEYLAAAAVRETREETAVRIRLGHPIDRIQYPIPTGTKRVAYWLGTELEQKRFKANS
                     EVDRRMWLTLPSAMKRMTYADERQLLEQAVTLPQTTPLLVVRHAKAMDRKHWSGKDQL
                     RQLNARGRRQSRQLVPLLDAYGVRDLASSTSLRCTSTLGPYAKSQQLDVRGWATLSEE
                     QAKAKPDAVRKLMKRLVIETAQGGVPRAICGHRPVLPLMLEALGVPDRPMLTAAVLVA
                     HLSPEGETVCVEWHKPRV"
     gene            2596..3687
                     /gene="pstS"
                     /locus_tag="PLF56_01870"
     CDS             2596..3687
                     /gene="pstS"
                     /locus_tag="PLF56_01870"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_013865610.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_component: GO:0009897 - external side of plasma
                     membrane [Evidence IEA];
                     GO_component: GO:0030288 - outer membrane-bounded
                     periplasmic space [Evidence IEA];
                     GO_component: GO:0055052 - ATP-binding cassette (ABC)
                     transporter complex, substrate-binding subunit-containing
                     [Evidence IEA];
                     GO_function: GO:0015415 - ATPase-coupled phosphate ion
                     transmembrane transporter activity [Evidence IEA];
                     GO_function: GO:0042301 - phosphate ion binding [Evidence
                     IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="phosphate ABC transporter substrate-binding
                     protein PstS"
                     /protein_id="HOQ52341.1"
                     /translation="MKISSIGAPVALLAAAALTLSACAANETANPGTGGTGTAAASTL
                     AGKGASSMSAAQQKWIADYQSAHPGVTINYSPDGSGAGREAFISGAVQYAGSDRPFKD
                     EELGKGKFGECTAESNALDLPIYVSPIAVIFNVDGVKNLKLDPDTLAGIFAGKITKWN
                     DPKIVATNPDVTLPDANITAVHRSDDSGTTNNFTDYLNQAAPTVWTEAASDTFPTAFG
                     GEAAKGTSAVVDAVTTGKNTIGYADASAAKELGHAQLQVGGKWLSPTAEAAAKIVDES
                     SVLPGRSEHDLALKLNRLSKDAYPAVLVSYALVCEAYKDANNAALVKGFIGYAASTEG
                     QKAAAEAAGSAPLSSSMAAKVKAAIDSIK"
     gene            3779..4753
                     /gene="pstC"
                     /locus_tag="PLF56_01875"
     CDS             3779..4753
                     /gene="pstC"
                     /locus_tag="PLF56_01875"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_016456661.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_component: GO:0005886 - plasma membrane [Evidence IEA];
                     GO_component: GO:0055052 - ATP-binding cassette (ABC)
                     transporter complex, substrate-binding subunit-containing
                     [Evidence IEA];
                     GO_function: GO:0015415 - ATPase-coupled phosphate ion
                     transmembrane transporter activity [Evidence IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="phosphate ABC transporter permease subunit PstC"
                     /protein_id="HOQ52342.1"
                     /translation="MTQSTREVTPAPTPERPAKTSRDRVGDRVFSGLATGSGITILVI
                     LALVAGFLLMQAFPALVTSPAELTAKGYGDFWSWILPFAWGTVWSSFWALLMAVPVSI
                     GIALFITHYAPRRLAGTLGYVIDLLAAVPSVVFGLWGSLVLAPAVQPLYLWLSAYLGW
                     IPFFSGQASGTGRTLLTASIVLAVMILPIVTSLSREVFLQTPVLHQEAALALGATRWE
                     MITMAVLPFGRAGVVSSVMLGLGRALGETMAVAMVLSGAPVITLEIITSQNPNTIAAV
                     IAQNFPEAYGLKTNQLIAGGLTLFVITLVVNSIARLIVSRYKEFSGAN"
     gene            4753..5817
                     /gene="pstA"
                     /locus_tag="PLF56_01880"
     CDS             4753..5817
                     /gene="pstA"
                     /locus_tag="PLF56_01880"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_013865612.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_component: GO:0005886 - plasma membrane [Evidence IEA];
                     GO_component: GO:0055052 - ATP-binding cassette (ABC)
                     transporter complex, substrate-binding subunit-containing
                     [Evidence IEA];
                     GO_function: GO:0015415 - ATPase-coupled phosphate ion
                     transmembrane transporter activity [Evidence IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="phosphate ABC transporter permease PstA"
                     /protein_id="HOQ52343.1"
                     /translation="MTALSTSTTVNPLTAGQLPRYATWVALAGSLLAGGVLDASLLGG
                     FDPGTTLFVGALIYLLVIYLLSRAVEGTRRAADRLATNLVTGAFLLALLPLVSLLWET
                     LSTGLPRFDLQFFSYSMRNVIGAGGGAVHALYGTLIVTGIATVISVPIGLMTAIYLVE
                     YGRGALARGITFFVDVMTGIPSIVAGLFAYALMAIVFGPGTINGFSGSLALSVLMIPI
                     VVRATEELLRIVPNELREASYALGVPKWRTIVSVVLPTSLSGIVSGVILAIARIIGET
                     APLMIAAGFTNSINTNPFANPMMTLPVFVYDQYAHPGVQREFYTERAWTGALTLIVLV
                     MVLNLIGRFIAARFSPKTGR"
     gene            5835..6614
                     /gene="pstB"
                     /locus_tag="PLF56_01885"
     CDS             5835..6614
                     /gene="pstB"
                     /locus_tag="PLF56_01885"
                     /EC_number="7.3.2.1"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_004810563.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_component: GO:0009898 - cytoplasmic side of plasma
                     membrane [Evidence IEA];
                     GO_component: GO:0055052 - ATP-binding cassette (ABC)
                     transporter complex, substrate-binding subunit-containing
                     [Evidence IEA];
                     GO_function: GO:0005524 - ATP binding [Evidence IEA];
                     GO_function: GO:0015415 - ATPase-coupled phosphate ion
                     transmembrane transporter activity [Evidence IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="phosphate ABC transporter ATP-binding protein
                     PstB"
                     /protein_id="HOQ52344.1"
                     /translation="MSKRIEVNDLNCFYGKFKAVEDVTMTIEPRSVTAFIGPSGCGKS
                     TFLRTLNRMHEVIPGAYVEGEVLVDGNNLYGPGVDPVNVRRQVGMVFQRPNPFPTMSI
                     KDNVLAGYRLNKQRMKASEAEELLEKSLRSANLWEEVKNRLDKPGSGLSGGQQQRLCI
                     ARAIAMQPEVLLMDEPCSALDPISTLAVEDLVQELKETYTIVIVTHNMQQAARVSDET
                     AFFNIAGTGAPGKLIEMGSTEKIFSTPDVQATEDYITGRFG"
     gene            6796..7674
                     /locus_tag="PLF56_01890"
     CDS             6796..7674
                     /locus_tag="PLF56_01890"
                     /inference="COORDINATES: protein motif:HMM:NF022169.2"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="DUF2510 domain-containing protein"
                     /protein_id="HOQ52345.1"
                     /translation="MSSAGWYPDPGGQPGMYRYWTGSSWSAAITNNPMQSPPADGIGA
                     PPRPVGQQTWGPDPQQRRRQTGWWLAVGAVVVVLAVIAYLAIRNLGGLLGGDPSAGSN
                     PTQAVCPKPVTTTASPAPDPNDGRVHGGALSYPRLGSPWSPPEVETRVPFGRDVLTQT
                     VLIQASYKPTGSWVASVLVGELIAGDGFFSPQQGSEIVVKCSMGVFYDDAEVVRDDKV
                     NKATKVDGKDAWLVETHLSFDIPNLNTKGELAIILIVATKADSSSIFYASIPDNADRQ
                     LLTDARAAMADLRVEG"
     gene            complement(7759..9009)
                     /locus_tag="PLF56_01895"
     CDS             complement(7759..9009)
                     /locus_tag="PLF56_01895"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_012918607.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_function: GO:0004553 - hydrolase activity, hydrolyzing
                     O-glycosyl compounds [Evidence IEA];
                     GO_process: GO:0005975 - carbohydrate metabolic process
                     [Evidence IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="glycoside hydrolase family 3 N-terminal
                     domain-containing protein"
                     /protein_id="HOQ52346.1"
                     /translation="MRPVSAAIGVVVATAVLTAGCSGGQLPSGQGPGESAGRVAETAS
                     RPASGSAAASPGPSTGGASGSAVPTSKVPTPVQCAAQLVEGMSLPERAGQLVMVGITD
                     AGSSELTALRRHHIGSVILMGTRTDGVVGVKRVTGRLAWKGQKVPVLVAADQEGGTVQ
                     RLKGPGFEAIPAASVQATWTNTTLRSRATRWGRQLAAAGVHWTLAPVADVVPASMGRR
                     NEPIGALRRGYGANPTTVAGKVTSFIDGMQTAGVATSAKHFPGIGRVVGHTDFAAGVR
                     DTATTPHDALLRPFQAAIESSVPSIMVSTVTYTRIDAHQPAVFSPSVIGIVRDLGFAG
                     VVISDDLGSARSVAAVPAKQRAIRFVKAGGDVAITADPTLADEFVDGIVAAAKSSGAV
                     ATAVERSATRMLTLKVEQGLLPCG"
     gene            complement(9006..10391)
                     /locus_tag="PLF56_01900"
     CDS             complement(9006..10391)
                     /locus_tag="PLF56_01900"
                     /EC_number="1.8.1.15"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_002545067.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="mycothione reductase"
                     /protein_id="HOQ52347.1"
                     /translation="MRHFDLCVIGSGSGNSIIDDQFSDQQVALVDKGERFGGTCLNVG
                     CIPTKMYVYPAELASSPERAERVGVSIGNVHADWPGIRDRIFGRIDPISEGGEQWRRS
                     NANVTLYREAASFTGPKQLLVGDETITADKFVLAAGSRPQIPVLKGMDDPRVAERVHT
                     SDDIMRLPGLPKSLVIIGGGFVAAEFAHVFASFGTEVTMLQRSEVLLRKEDADIAQAF
                     TEELAKRVNVRLKQRVIGIECTERDTVIVESVDPDGMHYDFEAEQVLIALGRVPNGDL
                     LNLAATGVTTHPDGRVVVDEHQQTVVKGIFALGDVSSASQLKHVANHEARVVQYNLLH
                     PRRMISADHRFIPHAVFSDPQIAAVGLTEQQAELTGRPFVTARQNYGTVAYGWAMEDE
                     SHFVKLIADPRTKKLLGAHILGPEATTLIQPLIQAMSFDLDVPSMARGQYWIHPALTE
                     VVENALLALDL"
BASE COUNT         1664 a         3655 c         3452 g         1731 t
ORIGIN      
        1 gccgctcgac ctgcgcggcc tgttctcgct ggccgacctt gaccgcgagg cgctgaagta
       61 cccggcgttc ctgccccgca cccaccccga gctggccgag gtcgaaaccg ccaaaccggc
      121 cgacatgttc gccgccctga agcaacgcga cgtgctgctg caccacccgt accactcgtt
      181 cgcgaccagc gtccagcgct tcgtcgagca ggccgccgcc gaccccgccg tgctcgcgat
      241 caagcagacg ttgtaccgca cgtccggcga ttcgccgatc gtggacgcac tgatcgatgc
      301 cgcccaggcc ggcaagcagg tactggccct ggtcgagatc aaggcccgtt tcgacgaaca
      361 ggccaacatc gcctgggccc gcaagctcga acgcgccggc tgccacgtgg tgtacgggct
      421 gatcggattg aagacccact gcaagctgtc gctggtggtg cgcgacgagc cggacgggct
      481 gcgccgctac tgccacatcg gcaccggcaa ctacaacccg aagaccgcgc gcatgtacga
      541 ggacgtgggg ctgctcaccg ccaacccggt ggtcaccgac gatgtcgccc ggctgttcaa
      601 ccacctctcg ggcatgggcc aggagaccca ctacaagcgg ctgctggtcg cgccgcacgg
      661 catccgccgc ggcttgatcg agttcatcga ggacgagatc gcccaccggc gcgcgggccg
      721 tccggccggc attcggatca aggtcaactc gatcgtggac gaggccgtga tcgacgccct
      781 gtaccgggcg tcgcaggccg gggtgcccgt cgacctgtgg gtgcggggca tctgcacggt
      841 gaagccgggc gtgcccggtt tgagcgacaa cattcgggtc cgctccattc tgggtcgctt
      901 tctggagcac agccgcctgt tctggttcgc caacgacggc cagcctcggg tgggcctggg
      961 ctcgaccgac ctgatgcacc gcaacctcga ccgccgggtc gaagtgctgg tctcactgac
     1021 caaccctcgc cacatcgccc agatcgagga gtggttcgac ctggccttcg acgagggcac
     1081 cgcgtcgtgg tggctcgacc agaacggttg gacgcaacgc accgccgacg ccgagggcga
     1141 gccgctgctc gacagccagg aacacctgat ccagctggtc agccggcgcc ccgacccccg
     1201 ggtcgtctga tggcgggttc gcgcctggtc gaggccgcgg gcgccgtcgt cctgcggcgg
     1261 gcgaagggtc gtcaggtgca ggtgctgttg atccatcgtc cgagctacaa cgactggtcg
     1321 ttgccgaaag gcaagctcga gaccgacgaa tacctggccg ccgccgccgt gcgcgaaacg
     1381 cgcgaagaga ccgccgtgcg cattcgcttg ggccacccga tcgaccgcat ccagtacccc
     1441 atcccgaccg gcaccaaacg cgtcgcgtac tggctgggca ccgaactcga acagaagcgg
     1501 ttcaaggcaa actccgaggt cgaccggcgc atgtggctga cgctgccgtc cgcaatgaag
     1561 cgcatgacct acgccgatga gcgacaactg ctcgaacagg cggtcaccct gccgcagacc
     1621 acgccgctgc tggtcgtccg gcacgccaag gcgatggatc gcaagcattg gtccggcaag
     1681 gaccaactgc ggcagctgaa cgcccgcgga cgccggcaga gcaggcagct cgtcccgcta
     1741 ttggacgcct acggggtgcg cgacctggcc tcgtccacgt cgctgcggtg caccagcacg
     1801 ttgggcccgt acgccaagtc tcagcaactg gacgtcaggg gctgggccac gctgtcggag
     1861 gagcaggcga aggcgaagcc ggacgccgtc cgcaagctga tgaagaggct ggtcatcgag
     1921 accgcgcagg gcggggtgcc gcgggcgatc tgcggccacc ggccggtgct gccgctgatg
     1981 ctggaggcgc tcggggtgcc ggatcgtccg atgttgaccg cggccgtgct ggtggcgcac
     2041 ctgtcccccg agggcgagac cgtctgcgtg gagtggcaca agccgcgggt gtagcgattc
     2101 gttggtcgag catgtcgaga cccgcccacc caccgttggt cgagaccgag gttgccgagc
     2161 gatctcgaga tcgccacacg atggcgggat acctcgccga tttcgtgttg caacccgaaa
     2221 gtgtccggct ttcccgccag tggtcagcga tctcgagatc gtcactggcc tcatgtcggt
     2281 tcctgatcga ttcatgcgac ggccttgacg ggtctcgaca cccgcgacca gccatgtctc
     2341 aacgtgacca tcgggcaccg acacagatcc cggcattcgc cgggatgacg gagggacgat
     2401 gacccggccc cgcccatccc tcgaccgacc ggacgcgttg tgcgcaagac ccccggcagg
     2461 cccacccgga acccgccacc gggacagtga tgccgagttc acccgctgtt catacaaggc
     2521 aacccattgc gtcgattggc ctccctagcg tgcgaggtgg agcgtcacgc tccacccgtt
     2581 cgtgaaagga aagacgtgaa gatctcttcg atcggtgcgc cggtggctct gctcgccgca
     2641 gctgcgctga ccctgtccgc ctgtgcggcc aacgagaccg ccaatcccgg caccggtggc
     2701 accggtaccg ccgcagccag cacgctggcc ggcaagggcg cctcgtcgat gtccgcggcg
     2761 cagcagaagt ggatcgccga ctaccagtcc gcccaccccg gcgtcaccat caactactcc
     2821 cccgacggct ccggtgcggg ccgcgaggcg ttcatctcgg gagccgtgca gtacgcgggc
     2881 tccgaccgtc cgttcaagga tgaagagctg ggcaagggca agttcggcga gtgcaccgcc
     2941 gaatcgaatg cgctcgacct gccgatctac gtgtcaccga tcgcggtgat cttcaacgtg
     3001 gacggcgtga agaacctcaa gctcgacccc gacaccctgg ccggcatctt cgccggcaag
     3061 atcaccaagt ggaacgatcc caagatcgtg gccaccaacc ccgacgtcac gctgcccgac
     3121 gcgaacatca ccgccgtgca ccgctcggac gactcgggca ccaccaacaa cttcaccgac
     3181 tacctcaacc aggccgcgcc gacggtgtgg accgaggccg cctcggacac gttcccgacg
     3241 gccttcggtg gtgaggctgc caagggcacc tcggccgtgg tcgacgctgt caccaccggc
     3301 aagaacacga tcggttacgc ggacgcatcc gcggccaagg agctgggcca cgcccagctt
     3361 caggtgggcg gcaagtggct gagcccgacc gccgaggccg cggccaagat cgtggacgag
     3421 tcctcggtgc tgcccggacg ctccgagcac gacctggcgc tcaagctgaa ccgcctgtcc
     3481 aaggacgcct acccggcggt gctggtgagc tacgccctgg tctgcgaggc ctacaaggac
     3541 gccaacaacg cggccctggt gaagggcttc atcggctacg ccgcttcgac cgagggccag
     3601 aaggcagccg cagaggccgc cggcagcgct ccgctgagca gctcgatggc cgccaaggtc
     3661 aaggccgcga tcgactcgat caagtgagcc gtccagcgtc cgtccgggtc acccggacgg
     3721 acgctggcgg gtcctggcgc ttatcgttct cagcagattc gagcgaaaga tgacttccgt
     3781 gacgcagtcc acccgggagg tgactcccgc accgacgccc gagcgtcctg ccaagacgtc
     3841 gcgcgaccgt gtcggcgatc gtgtgttcag cggcctggcc acgggctcgg gcatcaccat
     3901 cctggtgatt ctggcgttgg tggccggctt cctgttgatg caggcgttcc cggctctggt
     3961 gacctcgccc gccgaactga cggcgaaggg ctacggcgac ttctggtcgt ggatcctgcc
     4021 tttcgcctgg ggcacggtct ggtcgtcgtt ctgggcgctg ctgatggccg tgccggtgtc
     4081 gatcggcatc gccttgttca tcacccacta cgcaccacgc cggttggccg gcaccctggg
     4141 ctacgtgatc gacctgctgg ccgccgtgcc ctcggtggtc ttcggcctgt ggggttcgct
     4201 ggtgctggcg cccgcggtgc agccgttgta cctgtggctg agcgcctacc tgggctggat
     4261 cccgttcttc tcgggccagg cctccggcac cggacgcacc ctgctcaccg cctcgatcgt
     4321 gctggcggtg atgatcctgc cgatcgtcac ctcgctgtcg cgcgaggtct ttctgcagac
     4381 tccggtgctg catcaggagg cggcgctggc gctgggtgcc acccggtggg agatgatcac
     4441 gatggcggtg ctgcccttcg gccgcgccgg cgtggtgagc tcggtgatgc tcggcctggg
     4501 ccgcgcactc ggtgagacca tggcggtggc catggtgctg tcgggcgccc cggtgatcac
     4561 gctcgagatc atcacctcgc agaaccccaa caccatcgcc gcggtgatcg cgcagaactt
     4621 tcccgaggcc tacggcctca agaccaacca gctgatcgcc ggcggcctga cgctgttcgt
     4681 gatcaccctg gtggtcaaca gcatcgcgcg actgatcgtc agccggtaca aggaattctc
     4741 gggggccaac tgatgactgc gctcagcacc tcgaccacgg tcaatccgct caccgccgga
     4801 caactgccac gctacgccac ctgggtcgcg ctggccggct ccctgctggc cggtggcgtt
     4861 ctggacgcct cgctcctggg tggtttcgat ccgggcacca cgctgttcgt cggcgccctg
     4921 atctatctgc tggtgatcta tctgctttct cgcgccgtcg aagggacgcg gcgcgctgcc
     4981 gaccggctgg cgaccaacct ggtcaccggg gccttcctgc tggccctgct accgctggtc
     5041 tcgctgctgt gggagacgct gtcgaccggc ctgccgcgct tcgacctgca gttcttcagc
     5101 tactcgatgc gcaacgtgat cggtgccggc ggcggggccg tccatgccct gtacggcacc
     5161 ctgatcgtga ccggtatcgc caccgtgatc tcggtgccga tcggactgat gaccgccatc
     5221 tacctggtcg agtacggccg cggcgcactg gcacgtggca tcaccttctt cgtcgacgtg
     5281 atgaccggca ttccctcgat cgtggccggc ctgttcgcct acgcgctgat ggcgatcgtg
     5341 ttcggaccgg gcaccatcaa cggcttctcg ggctcgctcg cattgtcggt gctgatgatc
     5401 ccgatcgtgg tgcgcgccac cgaagagttg ctgcgcatcg tgccgaacga gttgcgcgag
     5461 gcgtcctacg cgctcggggt gccgaagtgg cgcacgatcg tgtccgtggt gctgcccacc
     5521 tcactctcgg gcatcgtgtc cggcgtgatc ctggcgatcg cccggatcat cggtgagacc
     5581 gcgccgctga tgatcgccgc gggcttcacc aactcgatca acaccaaccc gttcgccaac
     5641 ccgatgatga cgctgccggt cttcgtctac gaccagtacg cccaccccgg cgtgcagcgc
     5701 gagttctaca ccgaacgcgc gtggaccggc gctctcaccc tgatcgtcct ggtcatggtg
     5761 ctcaacctca tcggacgttt catcgccgcc cgcttcagcc cgaagaccgg gcgctgacca
     5821 aggagtcaca caccatgtcg aagaggatcg aggtcaatga cctcaactgc ttctatggca
     5881 agttcaaggc cgtcgaggac gtcacgatga ccatcgaacc acggtcggtg actgcgttca
     5941 tcggcccgtc cggctgcggc aagtcaacgt ttctgcgcac cctgaaccgc atgcacgagg
     6001 tgattccggg cgcctacgtc gagggtgagg ttctggtcga cggcaacaac ctgtacggcc
     6061 ccggcgtcga cccggtcaac gtgcgccgcc aggtgggcat ggtgttccag cggcccaacc
     6121 cgttcccgac catgtccatc aaagacaacg tgctggccgg ttaccggttg aacaagcagc
     6181 gcatgaaggc gtccgaggct gaagagctcc tggagaagtc gctgcgcagc gccaacctgt
     6241 gggaagaggt caagaaccgc ctggacaagc ccggctccgg cctgtccggc ggccagcagc
     6301 agcgtctgtg catcgcccgg gcgatcgcca tgcaacccga ggtgctgctg atggacgagc
     6361 cgtgctcggc cctcgacccg atctcgacgc tcgccgtcga ggacctggtg caggaactca
     6421 aagagactta cacgatcgtg atcgtgacgc acaacatgca gcaggccgcc cgggtgtcgg
     6481 acgagaccgc gttcttcaac atcgccggca ccggcgcccc gggcaagctg atcgagatgg
     6541 gctccaccga gaagatcttc tccacccccg acgtccaggc caccgaggac tacatcactg
     6601 gccgtttcgg ctgagctgta gggcgggggt gattcctccc ctgggtgccg agcgcccccc
     6661 taactttccg tggcctgcgt acctacgccg gcgctggatg gttagggggg cgctcggcgt
     6721 tgacccagcg gagaccggtt ctaggggtgc gattcctcac cctggcgctt ggcggcggct
     6781 gtctacactc gtgacgtgag cagcgcagga tggtatcccg acccgggtgg ccagccgggc
     6841 atgtaccgct actggacggg ttcgtcctgg tctgcggcga tcaccaacaa cccgatgcag
     6901 agcccgcccg ccgacggtat cggtgcgcca ccgcgtccgg tcggccagca gacctggggc
     6961 cctgacccgc agcaacgccg ccgtcagacc ggctggtggc tggccgtcgg tgccgtcgtg
     7021 gtggtgctgg cggtgatcgc ctacctcgcg atccgcaacc tcggcggtct gctggggggc
     7081 gacccgtccg cgggttccaa tcccacccag gccgtgtgcc ccaaaccggt gacgacgacc
     7141 gcgtcccccg cccccgaccc caatgacgga cgagtgcacg gcggcgctct ctcgtacccg
     7201 cggctgggta gtccctggag cccgcccgag gttgagaccc gcgtaccctt cggccgcgac
     7261 gtgctcaccc agaccgtgct gatccaggcc agctacaagc cgaccggtag ctgggtggcg
     7321 tcggtgctgg tgggcgaact catcgccggc gacgggttct tcagccccca gcagggttcc
     7381 gagatcgtgg tcaagtgcag catgggcgtc ttctacgacg acgccgaggt ggtgcgcgac
     7441 gacaaggtca acaaggccac caaggtcgac ggaaaggacg cctggctggt cgaaacccat
     7501 ctgtcgttcg acattccgaa cctgaacacc aagggtgaac tcgccatcat cttgatcgtg
     7561 gcgaccaagg ccgactcgtc cagcatcttc tacgcctcga ttcccgacaa cgccgaccgg
     7621 cagctgttga ccgacgcccg agccgccatg gccgacctgc gcgtcgaagg ctgagtgatc
     7681 cgtcgatcgc gacccacgcc gcaccatcgc cacgaccgtg gctggccacg ggttcgcgct
     7741 ggtgtccgac ccattcgctc agccgcaggg cagcagtccc tgctcgacct tcagggtcag
     7801 catgcgggtg gccgaccgtt cgacagccgt cgcgaccgct ccgctcgact tcgccgcggc
     7861 cacgatgccg tccacgaact cgtccgccag ggtcgggtcg gccgtgatcg cgacgtctcc
     7921 gccggccttc acgaaccgga ttgcccgctg cttggccggc accgcagcca ccgatctggc
     7981 ggacccgagg tcgtccgaga tgacgacccc cgcaaagccg aggtcgcgga cgatgccgat
     8041 caccgacggc gagaacaccg ccggttgatg cgcgtcgatg cgggtgtagg tgacggtcga
     8101 caccataatg ctcggcacgc tcgactcgat ggcggcctga aacgggcgca gcaacgcatc
     8161 gtggggcgtc gtcgccgtgt cgcgcacacc ggcggcgaag tcggtgtggc cgaccacgcg
     8221 tccgatgccg ggaaagtgct tggccgaggt agccacgccc gcggtctgca tgccgtcgat
     8281 gaacgaggtc accttgcccg ccaccgtcgt cgggttggcg ccgtagccgc gtcgcagcgc
     8341 accgatcggc tcgttgcgcc tgcccatgct cgccgggacc acgtcggcga ccggggccaa
     8401 cgtccagtgc actccggcgg cggcgagttg ccggccccac cgcgtggcgc gtgatcgcag
     8461 ggtggtgttg gtccaggtgg cctggacact cgcggcggga atcgcctcga agcccgggcc
     8521 cttcaggcgc tggacggtgc cgccctcctg gtcggccgcc accagcaccg gcaccttctg
     8581 gcccttccag gccaaccggc cggtgaccct cttcacccca accaccccgt ccgtgcgcgt
     8641 gcccatcagg atcaccgagc cgatgtggtg ccgacgcagc gcggtcaact cggacgaccc
     8701 cgcgtccgtg atgcccacca tcaccaactg ccccgcgcgt tcgggcagtg acatgccttc
     8761 gaccagttgc gccgcgcatt ggacgggcgt cggcaccttg ctggtcggca ccgccgagcc
     8821 gctcgcaccc ccggtggacg ggccgggcga cgcagcggcc gagccggacg ccgggcgtga
     8881 ggcggtttca gcgacgcggc cggccgactc accgggaccc tgaccgctcg gcagttgacc
     8941 accactacat ccggccgtca ggaccgcggt cgcgacgacg acgccgatcg ccgccgacac
     9001 cggcctcaca ggtcgagcgc gagcagcgcg ttctcgacca cctcggtgag cgccgggtgg
     9061 atccagtact ggccgcgggc catgctcggc acgtcaaggt cgaagctcat cgcctgaatc
     9121 agcggctgga tcagcgtggt ggcctcgggg cccagaatgt gcgcgccgag cagcttcttg
     9181 gtgcgcgggt cggcgatcag cttgacgaag tggctctcgt cttccatggc ccagccgtag
     9241 gccaccgtgc cgtagttctg ccgggcggtc acgaacggtc gccccgtgag ctcggcctgc
     9301 tgttcggtca gcccgaccgc ggcgatctgc gggtcgctga acaccgcgtg cggaatgaac
     9361 cggtggtcgg cgctgatcat gcgtcgcggg tgcagcaggt tgtactgcac cacccgcgcc
     9421 tcgtggttgg ccacgtgctt gagctggctg gcggacgaca cgtcacccaa cgcgaagatg
     9481 cccttgacca cggtctgctg gtgttcatcg acgaccaccc gaccgtcggg gtgggtggtg
     9541 accccggtgg ccgcgaggtt cagcaggtcg ccgttgggca cccgtccgag cgcgatcagc
     9601 acctgctcgg cctcgaagtc gtagtgcatg ccgtcggggt cgacgctctc gacgatcacc
     9661 gtgtcgcgct cggtgcactc gatgccgatc acgcgctgct tgagccgcac gttgacccgt
     9721 ttggccagct cttcggtgaa cgcctgggcg atgtcggcgt cttctttgcg caacagcacc
     9781 tcggatcgct gcagcatggt cacctcggtg ccgaagctgg cgaagacgtg cgcgaactcg
     9841 gcggccacga agccgccgcc gatgatcacc agcgatttgg gtagcccagg cagtcgcatg
     9901 atgtcgtcgg aggtgtggac gcgctcggcg acgcgcgggt cgtccatgcc cttcagcacg
     9961 ggaatctgcg gacgtgaccc tgccgcgagt acgaacttgt cggcggtgat ggtctcgtcg
    10021 ccgacgagca actgcttggg cccggtgaac gaggcggcct cgcggtagag ggtgacgttg
    10081 gcgttgctgc gccgccactg ctcgccgccc tcgctgatcg ggtcgatgcg gccgaagatg
    10141 cgatcgcgaa tgccgggcca gtcggcgtgc acgttgccga tgctgacccc gacccgctcg
    10201 gcccgctcgg gcgacgaggc caactcggcc gggtagacgt acatcttggt cgggatgcag
    10261 cccacgttga ggcaggtgcc gccgaagcgt tcgcccttgt cgaccagcgc gacctgctgg
    10321 tcactgaact ggtcgtcgat gatgctgttg cccgagcccg agccgatcac gcagaggtcg
    10381 aagtggcgca tgggccgatt gtgtcacccc ggtgtgacag gcggtgcgca tgcacggcta
    10441 cattgcctgg gtgcagagaa gatacgtcac cgtcgtggtg atgggcatgg ccctgctggt
    10501 cg
//
LOCUS       DAOSUG010000030        10081 bp    DNA     linear   ENV 10-JUN-2024
DEFINITION  MAG TPA_asm: Micropruina sp. isolate AMR_MDS_2195
            NODE_3685_length_10081_cov_4.592260, whole genome shotgun sequence.
ACCESSION   DAOSUG010000030 DAOSUG010000000
VERSION     DAOSUG010000030.1
DBLINK      BioProject: PRJNA925477
            BioSample: SAMN32864262
            Sequence Read Archive: SRR4236673
KEYWORDS    WGS; ENV; Metagenome Assembled Genome; MAG; Third Party Data; TPA;
            TPA:assembly.
SOURCE      Micropruina sp. (wastewater metagenome)
  ORGANISM  Micropruina sp.
            Bacteria; Bacillati; Actinomycetota; Actinomycetes;
            Propionibacteriales; Nocardioidaceae; Micropruina.
REFERENCE   1  (bases 1 to 10081)
  AUTHORS   Abdulkadir,N., Saraiva,J.P., Zhang,J., Stolte,S., Gillor,O.,
            Harms,H. and Rocha,U.
  TITLE     Genome-centric analyses of 165 metagenomes show that mobile genetic
            elements are crucial for the transmission of antimicrobial
            resistance genes to pathogens in activated sludge and wastewater
  JOURNAL   Microbiol Spectr 12 (3), e0291823 (2024)
   PUBMED   38289113
REFERENCE   2  (bases 1 to 10081)
  AUTHORS   Abdulkadir,N.
  TITLE     Direct Submission
  JOURNAL   Submitted (24-MAR-2023) Environmental Microbiology, Helmholtz
            Center for Environmental Research -UFZ, Permoserstr 15, Leipzig
            04318, Germany
COMMENT     The annotation was added by the NCBI Prokaryotic Genome Annotation
            Pipeline (PGAP). Information about PGAP can be found here:
            https://www.ncbi.nlm.nih.gov/genome/annotation_prok/
            
            ##Genome-Assembly-Data-START##
            Assembly Method        :: SPAdes v. 3.11.1
            Genome Representation  :: Full
            Expected Final Version :: Yes
            Genome Coverage        :: Not Applicable
            Sequencing Technology  :: Illumina MiSeq
            ##Genome-Assembly-Data-END##
            
            ##Genome-Annotation-Data-START##
            Annotation Provider               :: NCBI
            Annotation Date                   :: 05/04/2023 04:57:10
            Annotation Pipeline               :: NCBI Prokaryotic Genome
                                                 Annotation Pipeline (PGAP)
            Annotation Method                 :: Best-placed reference protein
                                                 set; GeneMarkS-2+
            Annotation Software revision      :: 6.5
            Features Annotated                :: Gene; CDS; rRNA; tRNA; ncRNA
            Genes (total)                     :: 2,776
            CDSs (total)                      :: 2,750
            Genes (coding)                    :: 2,714
            CDSs (with protein)               :: 2,714
            Genes (RNA)                       :: 26
            tRNAs                             :: 24
            ncRNAs                            :: 2
            Pseudo Genes (total)              :: 36
            CDSs (without protein)            :: 36
            Pseudo Genes (ambiguous residues) :: 0 of 36
            Pseudo Genes (frameshifted)       :: 2 of 36
            Pseudo Genes (incomplete)         :: 32 of 36
            Pseudo Genes (internal stop)      :: 2 of 36
            ##Genome-Annotation-Data-END##
FEATURES             Location/Qualifiers
     source          1..10081
                     /organism="Micropruina sp."
                     /mol_type="genomic DNA"
                     /submitter_seqid="NODE_3685_length_10081_cov_4.592260"
                     /isolate="AMR_MDS_2195"
                     /isolation_source="Activated sludge of municipal
                     wastewater treatment plants"
                     /db_xref="taxon:2737536"
                     /environmental_sample
                     /geo_loc_name="Canada"
                     /lat_lon="49.25 N 123.1 W"
                     /collection_date="2015-06-16"
                     /metagenome_source="wastewater metagenome"
                     /note="metagenomic"
     gene            <1..1967
                     /locus_tag="PLF56_01905"
     CDS             <1..1967
                     /locus_tag="PLF56_01905"
                     /EC_number="6.1.1.20"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_014847028.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_component: GO:0005737 - cytoplasm [Evidence IEA];
                     GO_function: GO:0000049 - tRNA binding [Evidence IEA];
                     GO_function: GO:0000287 - magnesium ion binding [Evidence
                     IEA];
                     GO_function: GO:0004826 - phenylalanine-tRNA ligase
                     activity [Evidence IEA];
                     GO_function: GO:0005524 - ATP binding [Evidence IEA];
                     GO_function: GO:0016740 - transferase activity [Evidence
                     IEA];
                     GO_process: GO:0006432 - phenylalanyl-tRNA aminoacylation
                     [Evidence IEA]"
                     /codon_start=3
                     /transl_table=11
                     /product="phenylalanine--tRNA ligase subunit beta"
                     /protein_id="HOQ52348.1"
                     /translation="CLSIRGVAREAGQALGVPFTDVVDRVVPAPVSAGYPVVLDDPAC
                     SLFVALGVSGIDPAATSPRWLQRRLSAAGMRSISLPVDVTNYVMLEVGQPLHAYDARK
                     LQGPIRVRKASENESIVTLDDVRRSLSSDDLLITDDSGPIGIAGVMGGASTECDPATT
                     GIVLEAAAFDPPTISRSMRRHALPSEASKRFERGTDPAAAYAAAHLAARLLVELGGGT
                     LSSDETVVGAVAPLPGQRLRTDLPSQILGVEVPAARVVQILRAGGVAVTDDGDGTLQV
                     QPPTWRRDLRDPYDYVEEVGRKIGFDEIPSIVPTPPTGRGLTRAQVIRRAVTHALAGA
                     GFTQVLLLPFASGDDLDRLGLPADDRRRATVRLANPLAETSPFLRTSLLPGLFAAVQR
                     NTSRGNDDLALFESGAVFRAGSGSAAPVPGVESRPSEAELAAMEAALPAQPWQLGAVL
                     TGAWRPAGWAGAVEPASWQHALAFADVAAAAAGVTVRRVADDHAPFHPGRCAKLLVAG
                     ADGAEVLFGHAGELHPLVCRAFGLPARTAAVEVSLDVLIEVAPGPGTIAPVSPHPVAK
                     EDVALVVDESVSAGEVQVALAAGAGELLESIRLFDIYRGAQIAEGSKSLAFALRFRAA
                     DRTLKVAEISAARDAAVAEAVRLFGAVQRA"
     gene            complement(2047..2679)
                     /locus_tag="PLF56_01910"
     CDS             complement(2047..2679)
                     /locus_tag="PLF56_01910"
                     /inference="COORDINATES: protein motif:HMM:NF012655.2"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_function: GO:0003677 - DNA binding [Evidence IEA];
                     GO_function: GO:0003700 - DNA-binding transcription factor
                     activity [Evidence IEA];
                     GO_process: GO:0006355 - regulation of DNA-templated
                     transcription [Evidence IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="TetR/AcrR family transcriptional regulator"
                     /protein_id="HOQ52349.1"
                     /translation="MPLTDDVPVKRKRNRQAEASAETRTRILDAAVSAFTEQGYNATS
                     IREVSQRACMSHTGVLHHFPDKAALLEAVLDRYLAQGLQKVELDPTDGLSVLNGLVVI
                     ARHDVGDPASLRMYRMLSTEALAPTHPAHGYFRRWYAQVRSWAIGGLNDLQRRGLYAQ
                     PGLSVEAASVQIAGMRDGLDPQWLLDPDEVDLVEVVRAQLQLYSTEELTE"
     gene            complement(2813..3664)
                     /locus_tag="PLF56_01915"
     CDS             complement(2813..3664)
                     /locus_tag="PLF56_01915"
                     /inference="COORDINATES: protein motif:HMM:NF019310.2"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_function: GO:0022857 - transmembrane transporter
                     activity [Evidence IEA];
                     GO_process: GO:0055085 - transmembrane transport [Evidence
                     IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="MFS transporter"
                     /protein_id="HOQ52350.1"
                     /translation="MPDQFAPERRGVVSGLIGIAGSAGAILGLFIGSLLSPNLALMIM
                     VPALLAAALNLLLAVVVKDDPIRAQDRPPLALKDVFGTFWVSPRRYPDFALAFASRFA
                     VFCAIAAVNAYQAVYMIMGLHISATEVAGKIFVANLVSGVLAIGFATVLGKVSDKVGR
                     RKPFVVIAAAVFAVGLVIIAAASSFETFLIGAAIMGIGMGVYLAVDFALITQVLPDPN
                     NPAKDLGIMNLASSLPNIMVPAVAPLLLAIGATAASPNNFVVFFGAAAIAGAIGAALI
                     LPIKRVR"
     gene            complement(3616..4113)
                     /locus_tag="PLF56_01920"
     CDS             complement(3616..4113)
                     /locus_tag="PLF56_01920"
                     /inference="COORDINATES: protein motif:HMM:NF024742.2"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="MFS transporter"
                     /protein_id="HOQ52351.1"
                     /translation="MTNSTAPSLTESSPGGRAASTPLFRNPGAGLTAVLAAAAISMMA
                     SLLSVGILTMPLKATEIDAASSTTLLAVANAFAGLVALLLNPVIGRISDRTPGRFGRR
                     RPYIVLGAALLVLGAYIVLQASGVAMLALGWVVMTIGQIAGHPAHSRSCPTSSPPSAA
                     AWSPV"
     gene            4251..>5454
                     /locus_tag="PLF56_01925"
     CDS             4251..>5454
                     /locus_tag="PLF56_01925"
                     /inference="COORDINATES: protein motif:HMM:NF020120.2"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="family 78 glycoside hydrolase catalytic domain"
                     /protein_id="HOQ52352.1"
                     /translation="MTTAIAPAPSTLSGDAAVRQALCGATWIGPNEGTPAPAGRRPVQ
                     LLRTRVDLPADRPLHLHATAHGVYEVFVDGIRLGEAELSPGFSAYRKRLEVQTYDLTG
                     LVTPGVTTITVLLSDGWYRGRHGFERRADGFGTRTAVLLAVTGADGEPLAVSDAGWQS
                     RPSHLWRADLMEGQAVDLRALDPGWFTGADDGGGWRPVLMSDDPVCADRDRLVAPAGP
                     PVRRIEELAPVALTTPRPGTVVVDLGQVINGWVRLPEVGPSGTHLVLTHGEAVDGSGL
                     VTTDHLRAFVFATGDLLPAGQVDEVISAGRPGEGFEPRHTTHGFRYVQVDGVPDGLDL
                     GGLRGVVVHSDIARTGEFGCSDERLERLHEVVRWSLRTNACAIPTDCPQRERSGFTGD
                     WQIFVGTAA"
     assembly_gap    5455..5554
                     /estimated_length=100
                     /gap_type="within scaffold"
                     /linkage_evidence="paired-ends"
     gene            <5555..6853
                     /locus_tag="PLF56_01930"
     CDS             <5555..6853
                     /locus_tag="PLF56_01930"
                     /inference="COORDINATES: protein motif:HMM:NF036578.2"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="alpha-L-rhamnosidase C-terminal
                     domain-containing protein"
                     /protein_id="HOQ52353.1"
                     /translation="RERSGFTGDWQIFVGTAALMADVQAFSRRWLRDLAADQWADGRV
                     PTIVPNPAGDGPSGVLFEDASAGSAGWGDAAAIVPWELWRAYGDADALAEQFPAMRRW
                     VDYAAGCAASARHPERAAARPEPCGHEQYLWDTGFHFGEWLEPDVPPAPDARVDHGIV
                     ATAYLHRSAAIAAATAAVLGLDDLAGQYRVIAEGARAAWQAEYLLPDGRLTQERQAHY
                     VRALAFGLVPASQRQAAADRLAELVHAADDRLNTGFLATGMLLQVLADHGHAELAHTL
                     LTRTGSPSWLGMLEAGATTMWEYWDGIGPDGTAKGSLDHYSKGAVAGFLHTHLAGLRL
                     PEFPAADEAGYRRVLIAPTMLPGITSARTTQRTAAGPIEVAWRLRGTRFELSVDLPPG
                     VEASIDLPPGVEASIDLPDGSRAAGVDGRHVFNVELPQST"
     gene            7090..7638
                     /locus_tag="PLF56_01935"
     CDS             7090..7638
                     /locus_tag="PLF56_01935"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_007076821.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_function: GO:0008703 -
                     5-amino-6-(5-phosphoribosylamino)uracil reductase activity
                     [Evidence IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="dihydrofolate reductase family protein"
                     /protein_id="HOQ52354.1"
                     /translation="MATIFYTATTLDGFLADPHDSLEWLFRQHQNAGGPFDYEVFIAD
                     VGALAMGATTYEWVRTHLADSAEPWPYRQPTWVFTHRALAPIPGADVRLTQAPVAEVH
                     AELTAAGGQNVWIVGGGALAADFARAGLLDEVVVSIAPVTLGAGRPLLTGRFDLELIE
                     TVRNVDFICARYRVLGPLTEDR"
     gene            complement(7622..8014)
                     /locus_tag="PLF56_01940"
     CDS             complement(7622..8014)
                     /locus_tag="PLF56_01940"
                     /inference="COORDINATES: protein motif:HMM:NF012792.2"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_function: GO:0008080 - N-acetyltransferase activity
                     [Evidence IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="GNAT family N-acetyltransferase"
                     /protein_id="HOQ52355.1"
                     /translation="MQEALDNDSFEIPALHEDLDTVRAGIAQWHTWLLRRDGRLIGSV
                     RAQLDGDTWDIGRLMAAPDLAGHGLGRMLLEHAERQAPDGVARYRLFTGAGSARNLRL
                     YRAAGYVETGEPAPPVAVMLAKPVSDPR"
     gene            complement(8135..8857)
                     /gene="cobA"
                     /locus_tag="PLF56_01945"
                     /pseudo
     CDS             complement(8135..8857)
                     /gene="cobA"
                     /locus_tag="PLF56_01945"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_002523090.1"
                     /note="incomplete; partial in the middle of a contig;
                     missing C-terminus; Derived by automated computational
                     analysis using gene prediction method: Protein Homology.
                     GO_function: GO:0004851 - uroporphyrin-III
                     C-methyltransferase activity [Evidence IEA];
                     GO_process: GO:0019354 - siroheme biosynthetic process
                     [Evidence IEA]"
                     /pseudo
                     /codon_start=1
                     /transl_table=11
                     /product="uroporphyrinogen-III C-methyltransferase"
     gene            complement(9336..>10081)
                     /locus_tag="PLF56_01950"
     CDS             complement(9336..>10081)
                     /locus_tag="PLF56_01950"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_011753991.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_function: GO:0008483 - transaminase activity [Evidence
                     IEA];
                     GO_function: GO:0030170 - pyridoxal phosphate binding
                     [Evidence IEA]"
                     /codon_start=3
                     /transl_table=11
                     /product="aminotransferase class III-fold pyridoxal
                     phosphate-dependent enzyme"
                     /protein_id="HOQ52356.1"
                     /translation="SGWWGLERAAAERSSSVERASMVEPIETPGATAQHSSLVDPVET
                     PDPTVQRSSPVERPSVAEPSETPSPTSSPHEAPDLFTFGKVVGGGMPLAAIGGRAEVM
                     DLLAPLGPVYQAGTLSGNPLATAAGIATLRLADRAVYAAVDAASAAVQAAITDALSAE
                     GVPHLVQSAGSLFTVFFGDFDGPIVDYPGAQRQNATAFARFFHSMLDAGVALPPSGYE
                     AWFLSAAHDDAALDRIVAALPAAARAARA"
BASE COUNT         1420 a         3456 c         3661 g         1444 t
ORIGIN      
        1 actgcctgtc gatccgaggg gtggcccgcg aggccgggca ggcgctcggc gtgccgttca
       61 ccgacgtcgt ggacagggtc gttccggcac cggtctcggc cggctacccc gtggtgctgg
      121 acgacccggc gtgctcactc ttcgtcgccc tgggggtctc gggcatcgac ccggccgcga
      181 cctcgccgcg ctggttgcag cggcggctga gcgcggcggg catgcggtcg atctcgctac
      241 ccgtggacgt gaccaactac gtgatgctcg aggtcgggca gcccttgcat gcctacgacg
      301 cacgcaagct gcaggggccg atccgggtgc gcaaggcgtc cgagaacgag agcatcgtga
      361 ccctggacga cgtgcggcgc tcgctgtcgt ccgacgacct gctgatcacc gacgattccg
      421 gcccgatcgg catcgccggc gtgatgggcg gtgccagcac cgagtgcgat ccggcgacca
      481 ccggcatcgt gctcgaggcg gccgcgttcg atccccccac gatctcgcgc tcgatgcgcc
      541 ggcacgcgct gccgtccgag gcgtccaagc gcttcgagcg gggcacggat ccggcggccg
      601 cctatgccgc cgcgcacctg gccgcgcggc tgctggtcga gttgggcggg ggcacgctgt
      661 cgtccgacga gacggtggtg ggcgccgtgg ccccgctgcc ggggcagcgg ctgcgcaccg
      721 acctgccctc gcagattctg ggtgtcgagg tgccggccgc gcgggtggtg cagattctgc
      781 gggccggcgg cgtcgcggtg accgacgacg gcgacggcac gctgcaggtg cagccgccga
      841 cctggcgccg cgacctgcgc gacccgtacg actacgtcga agaggtcggc cgcaagatcg
      901 gtttcgacga gatcccgtcg atcgtgccga ccccgccgac cggacggggg ctgacccggg
      961 cccaggtgat ccggcgggcg gtcacccatg cgctggccgg cgccggcttc acgcaggtgt
     1021 tgctgttgcc gttcgcctcg ggtgacgacc tcgatcggct cgggctgcct gcggacgacc
     1081 ggcggcgggc gaccgtccgg ttggcgaatc cgctggccga gaccagcccg tttctgcgca
     1141 cctcgctgct gcccgggttg ttcgccgcgg tgcagcgcaa cacctcgcgt ggcaacgacg
     1201 acctggcgct gttcgagtcg ggggcggtgt tccgggccgg ctcgggttcg gcggcgccgg
     1261 tgccgggtgt cgagtcgcgt ccgtctgagg cggagttggc ggcgatggag gctgcgctgc
     1321 cggcgcagcc ctggcagctc ggtgcggtgc tgaccggcgc gtggcgtccg gccggctggg
     1381 cgggcgccgt cgagccggcc tcgtggcagc atgcgctggc cttcgccgac gtggcggcgg
     1441 cagcggccgg tgtgacggtg cggcgggtcg cggacgacca tgcaccgttc catcccggac
     1501 gctgcgcgaa actgctggtg gccggcgcgg acggggccga ggtgctgttc ggccatgccg
     1561 gtgaactgca tccactggtg tgccgggcct tcgggctgcc cgcgcgcacg gcggccgtcg
     1621 aggtcagcct cgacgtgctg atcgaggtgg cgccggggcc gggcacgatc gcgccggtgt
     1681 cgccgcaccc ggtggcgaaa gaggatgtcg ccctggtggt cgacgagtcg gtcagcgcgg
     1741 gcgaggtgca ggttgccctg gcggcagggg cgggtgagtt gctggagtcg atccggctgt
     1801 tcgacatcta ccgcggcgcg cagattgccg agggcagcaa gtcgctggcg ttcgcactgc
     1861 gattccgggc cgccgatcgc acgctcaagg tggcggagat ctccgccgcc agggacgcgg
     1921 cggtggccga ggccgtccga ttgttcgggg cggtccagcg ggcctaatgg tgtgtccctc
     1981 acccggtagg tgattggggg gacacgccac tagcaggtcg tgcagcccga gcgcttgcgc
     2041 cgaggcctac tccgtcagtt cctccgtcga atacaactga agctgggcgc gcacgacctc
     2101 gaccaggtcc acctcgtcgg ggtcgagcag ccactgcggg tcgagaccgt cacgcatgcc
     2161 ggcgatctgt accgacgccg cctcgacgct gagcccgggc tgcgcgtaca ggccgcgtcg
     2221 ttgcaggtcg ttgagcccgc cgatcgccca cgaccgcacc tgcgcgtacc agcggcgaaa
     2281 gtacccgtgg gcggggtggg tgggcgcgag cgcctcggtg gagagcatcc ggtacatgcg
     2341 caggctggcc gggtccccta cgtcgtggcg cgcgatgacg accagtccgt tgagcaccga
     2401 cagcccgtcg gtggggtcga gttcgacctt ctgcagtccc tgggcgaggt agcggtccag
     2461 caccgcttcg agcagggcgg ccttgtccgg aaagtggtgc agcacgcccg tgtgcgacat
     2521 gcaggcacgc tgggagacct cgcggatcga ggtcgcgttg taaccctgct cggtgaacgc
     2581 ggacaccgcg gcgtcgagga tccgggtacg ggtctcggcg ctggcctcgg cctgccggtt
     2641 ccgcttgcgc ttgaccggca cgtcgtcggt gagtggcatc gcgcagggtc tccttcgtcc
     2701 ggccgggaaa gacggtgtga gttcaggcag tgaacacagg gcccgaactc acaccgtctg
     2761 gacgggccat gccgtcccac cctaatggcg cgtgggtgct gcgtccgccg ggtcagcgga
     2821 cccgtttgat gggcaggatc agggctgcgc cgatcgctcc ggcgatcgcg gcggcgccga
     2881 agaacaccac gaagttgttg ggcgacgcgg cggtggcgcc gatggcgagc agcaacggag
     2941 cgacggccgg caccatgatg ttgggcagcg agctggccag gttcatgatg cccaggtcct
     3001 tggcggggtt gttcgggtcg ggcagcacct gggtgatcag cgcgaagtcg accgcgagat
     3061 agacacccat gccgatgccc atgatcgcgg cgccgatcag gaacgtctcg aacgacgatg
     3121 cggcagcgat gatcaccagg ccgaccgcga agacggcggc ggcgatcacc acgaagggct
     3181 tgcggcgccc caccttgtcg gacaccttgc ccagcacggt cgcgaagccg atcgccagca
     3241 cacccgagac caggttggcc acgaagatct tccctgccac ctcggttgcc gaaatgtgca
     3301 ggcccatgat catgtacacg gcctggtagg cgttgaccgc ggcgatcgcg caaaagacgg
     3361 cgaaccggct ggcgaaggcc agtgcgaagt cggggtagcg gcgcgggctg acccagaagg
     3421 tgccgaagac gtctttcagg gccagcggcg gacggtcctg cgcccgaatc gggtcgtcct
     3481 tcacgacgac cgccagcagc aggttgagcg ctgccgcgag cagggccggc accatgatca
     3541 tcagggccag gttgggcgac agcaacgaac cgatgaacag gccgagaatg gcgccggcac
     3601 taccggcgat gccgatcaga ccggagacca cgccgcggcg ctcgggggcg aactggtcgg
     3661 gcacgatcgc gaatgcgccg gatggccggc gatctggccg atggtcatca ccacccagcc
     3721 gagggcgagc atcgccacgc cggaggcctg cagaacgatg tacgccccca gcaccagcag
     3781 tgcggcgccc agcacgatgt aggggcggcg gcggccgaac cggcccggcg tgcggtcgct
     3841 gatccggccg atcaccgggt tgagcagcag cgcgaccagg ccggcgaacg cgttcgcgac
     3901 ggcgagcagg gtggtggacg aggcggcgtc gatctcggtg gccttgagcg gcatcgtcag
     3961 gatgccgacc gacagaagcg aggccatcat cgagatggcc gcggcggcca gaaccgcggt
     4021 gagcccggcg cccgggttgc ggaacagggg agtggaggcg gcacggccgc ccgggctcga
     4081 ttcggtcagc gagggtgcgg tcgagttggt catggatgaa gctcctttgc gtcatgtagc
     4141 gcaggtgcac tcccgccggc tgcaacagaa ggtagcaata cacaaaccat cttgacaaga
     4201 ggtacctaac aagtggtaga aatagatcga cacctcaccg aggaggacct gtgaccaccg
     4261 ccatcgcccc cgccccgtcc acgctgagcg gtgatgccgc cgtccgccag gcattgtgcg
     4321 gcgccacctg gatcggcccg aacgagggca cacccgcgcc ggcggggcgg cgtccagtcc
     4381 agttgctgcg cacccgggtc gacctgcccg ccgatcgtcc gctgcacctg cacgccacgg
     4441 cccacggcgt ctacgaggtg ttcgtcgacg gcatccggct gggtgaggcc gaactcagcc
     4501 ccggcttctc cgcgtaccgc aagcggctcg aggtgcagac ctacgacctg accgggttgg
     4561 tgacgccggg ggtgaccacg atcaccgtgc tgctgtcgga tggctggtat cgcggccggc
     4621 acggcttcga gcgacgcgcg gacgggttcg gcacccgcac ggcggtgctg ctggccgtga
     4681 ccggtgcgga cggcgaaccg ctggcggtct cggacgccgg ctggcagtcc cggccgtcgc
     4741 acctttggcg ggccgacctg atggagggcc aggcagtcga cctgcgggcc ctcgaccccg
     4801 gctggttcac cggtgcggac gacggcggcg gctggcgtcc ggtgctgatg tcggacgacc
     4861 cggtctgcgc ggaccgcgac cggctggtgg cgccggccgg cccgccggtg cgccgcatcg
     4921 aggagctggc tcccgtcgcg ctgaccaccc cgcgccccgg caccgtcgtg gtcgacctcg
     4981 gccaggtgat caacggctgg gtgcggctgc ccgaggttgg cccgtccggc acccacctgg
     5041 tgctcaccca cggcgaggcg gtggacggct ccggcctggt caccaccgac caccttcgcg
     5101 cgttcgtgtt cgccaccggc gacctgctgc ccgccggcca ggtcgacgag gtgatttcgg
     5161 ccggacgtcc cggcgaaggc ttcgagcccc ggcacaccac ccacggcttc cgctacgtgc
     5221 aggtcgacgg ggtgccggac ggactcgacc ttggcggact tcgcggtgtg gtggtgcaca
     5281 gcgacatcgc ccgcaccggc gagttcggct gctcggacga gcgcctggag cgactgcacg
     5341 aggtggtccg ctggagcctg cgcaccaatg cctgcgccat tccgaccgac tgcccgcagc
     5401 gcgagcggtc cgggttcacc ggcgactggc agatcttcgt cggcaccgcc gcgcnnnnnn
     5461 nnnnnnnnnn nnnnnnnnnn nnnnnnnnnn nnnnnnnnnn nnnnnnnnnn nnnnnnnnnn
     5521 nnnnnnnnnn nnnnnnnnnn nnnnnnnnnn nnnncgcgag cggtccgggt tcaccggcga
     5581 ctggcagatc ttcgtcggca ccgccgcgct gatggccgac gtccaggcct tctcgcggcg
     5641 ctggctgcgc gatctggccg ccgaccagtg ggcggacggc cgggtcccga cgatcgtgcc
     5701 caacccggcc ggcgacgggc cgtccggggt gctgttcgag gacgcatccg ccgggtcggc
     5761 cggctggggc gacgccgccg cgatcgtgcc gtgggagttg tggcgcgcct acggagacgc
     5821 cgacgcgctg gcggaacagt ttcctgcgat gcgccgctgg gtcgactacg cggcgggctg
     5881 tgcggcgtcc gcccggcacc ccgagcgggc cgcggcacgt cccgagccgt gcggccatga
     5941 gcaatacctc tgggacaccg gcttccactt cggcgaatgg ctcgaaccgg atgttcctcc
     6001 ggcacccgat gcgcgcgtcg atcacggcat cgtcgccacc gcctacctgc accgctcggc
     6061 ggccatcgcc gccgccaccg ctgcggtgct ggggctcgat gacctcgccg gtcagtaccg
     6121 agtcatcgcc gagggtgccc gcgccgcctg gcaggccgag tacctgctgc cggacggtcg
     6181 gctgacccag gaacgccagg cccactacgt gcgggcgctc gctttcgggc tggtgccggc
     6241 ttcgcagcga caggccgccg ccgaccggct cgccgagctg gtgcacgccg cggacgaccg
     6301 gctgaacacc ggctttctgg ccaccggcat gctgctgcag gtactggccg accacggtca
     6361 cgccgagctg gcccacacgc tgctgacccg caccggcagc ccgtcctggc tgggcatgct
     6421 cgaggccggc gccaccacga tgtgggagta ctgggacggc atcggcccgg acggcaccgc
     6481 gaaggggtcg ctcgaccact acagcaaggg ggcggtggcc ggctttctgc acacccacct
     6541 ggccggcctg cggctgccgg agtttccggc ggcggacgag gcggggtatc gccgggtgct
     6601 catcgcgccg acgatgttgc ccggcatcac ctcggcccgc acgacgcagc gcaccgcggc
     6661 gggcccgatc gaggtggcct ggcgcctgcg cggaaccagg ttcgagctga gcgtcgacct
     6721 gccgccgggt gtcgaggcgt ccatcgactt gccgccgggt gtcgaggcgt ccatcgactt
     6781 gccggacggc agccgcgccg cgggggtcga cggtcgccac gtgttcaatg tggagctacc
     6841 ccaatcgacg taacgagcgc tcggacgatc aggcagatca ccgatccgat gcctcgagat
     6901 gtgcagcaga tcgcccgcgc gatgcgccgc tgccgacgct gactgatcgg cggtccgtag
     6961 ggaagtgctg atcaaggcgc tatccgttgg ttgagcgtgt cgaaacccct cgtctcaggg
     7021 ttctcgacaa gctcgaccag cgagagatct agaattgccg ggttcgggca ggcgaactgg
     7081 gaggtaccga tggcgacgat cttctacacc gcgaccaccc tggacggctt tctggccgac
     7141 ccgcacgact cgctggagtg gctctttcgc cagcaccaga acgccggcgg acccttcgac
     7201 tacgaggtgt tcatcgccga tgtcggggca ctggcgatgg gggcgaccac ctacgagtgg
     7261 gtgcgcaccc acctcgccga ctcggccgaa ccgtggccct accggcagcc gacctgggtg
     7321 ttcacccatc gcgcgctggc accgatcccc ggtgcggacg tccgcctcac ccaggcgccg
     7381 gtggctgagg tgcatgccga gctgaccgcc gccgggggtc agaacgtctg gatcgtcggc
     7441 ggtggcgcac tggccgccga cttcgcccgg gcggggctgc tcgacgaggt cgtggtcagc
     7501 atcgcgccgg tcaccctggg agccggacgt ccgctgctca ccggccgctt cgatctcgag
     7561 ctgatcgaga ccgtccgcaa cgtcgacttc atctgtgccc gctaccgggt gctcggcccg
     7621 ctcaccgagg atcgctgacc ggcttggcca gcatcaccgc caccggcggg gctggctcgc
     7681 cggtctcgac gtatccggcc gcgcggtaga gccgcaggtt gcgcgcgctg cccgcaccgg
     7741 tgaacaaccg atacctggcg acgccgtccg gcgcctgccg ctcggcgtgc tccagcagca
     7801 tccggccgag cccgtgtccg gccaggtcgg gggcggccat cagccggccg atgtcccacg
     7861 tgtcgccgtc cagttgcgca cgcaccgagc cgatcagccg tccgtcgcgg cgcagcagcc
     7921 acgtgtgcca ctgagcaatc ccggcgcgca cggtgtcgag gtcctcgtgc agcgccggaa
     7981 tctcgaaact gtcgttgtcg agggcctcct gcatccagca ggcccgctgc agcaccagca
     8041 gttcgcccgc gtccgatgac gtggccggcc gcagctcgtc ggtctgccca gccgcgggct
     8101 caggacccat gcccgccgcc ggtgcgtaga tcgcgagctc cagcgcagcc acgtcgccga
     8161 tcaccgcgat cgcgggcgga cggatgccgg cggcgttcgc gcggtcggcg atgtcggcgg
     8221 cgcgtccgcg caccgtgcgc atggtgggca gccaggcgtc cgcgatcacc gccgccgggg
     8281 tgccggcaga cagcccgccc gccaccagct cggcgcagat cgggcccagg ttcgcgacac
     8341 ccatcagcac cacgatggtg tggttggcgc gggccaacgc agcccagtcg gtgctggacg
     8401 ccttgtgccg gggcggcaca tgcccagaaa ccaccgtgaa cgcctgcacg atgccgcggt
     8461 gcgtcaccgg gatgcccgcc agtgccggcc ccgcgatcgc cgacgacact cccggaatga
     8521 cctccaccgg aatacccgcc tcggcgcagg ccagcacctc ttcgccaccg cgcccgaaca
     8581 cgaaggggtc gccgcccttg aaccgcacca cgtagcgtcc ggcggcggcg tgctcgacca
     8641 gcaggtcgtt gatgcgttgc tgcgaagtgg atcgcccacc agggatcttg cccacgtcga
     8701 ccatttcggt gtccggacgg cacccggcga gggacgcggc cggcgccagg tggtcgtaca
     8761 gcaccacgtc ggcccggcgc agggcggccg caccggccag cgtcagcaac cctggatcac
     8821 ccggcccgcc gccgaccagc accacccgac cggtcaccgc ccactcctcg gttcggcgtg
     8881 gcgagatccc ggcgttcgcc gggatgacag aggaagtgtc atgccgggtt tgcccggggt
     8941 tcccggcgtt cgccgggatg acgaggcggg cgttcgccgg gatgaaggag gaggtgtcat
     9001 gccgggcttg atcggggttc ccggcgttcg ccgggatgaa ggaggaggtg tcatgccggg
     9061 cttgcccggg gttcccggcg ttcgccggga tgaaggagga ggtgtcatgc cgggtttgcc
     9121 cggggttccc ggcgttcgcc gggatgaagg aggaggtgtc atgccgggct tgatcggggt
     9181 tcccggcgtt cgccgggatg aaggaggagg tgtcatgccg ggcttgcccg gggttcccgg
     9241 cgttcgccgg gatgaaggag gaagtgtcat cccgggcttg ccccgggatc tcgaccgcac
     9301 cagagatcgg ggcgtgcgca cggacgaagg ctgagtcatg cccgtgctgc ccgcgcggcg
     9361 gcgggcaacg ccgcgacgat gcggtccagg gccgcgtcgt cgtgcgccgc agacaggaac
     9421 catgcctcgt agccggacgg gggcagcgcc acacccgcgt ccagcatgga gtggaagaac
     9481 cgcgcgaaag cggtcgcgtt ctgccgctgc gcacccggat agtcgacgat cgggccgtcg
     9541 aagtcgccga agaacaccgt gaacaacgac ccggccgact gcaccagatg cggaacaccc
     9601 tcggcgctca gggcatcggt gatcgccgcc tgtacggcag ccgaggctgc gtccacggcg
     9661 gcgtacaccg cccggtcggc cagtcgaagc gtggcgatac cggccgcggt ggccagtggg
     9721 ttacccgaca gggttccggc ctggtagacc ggcccgagcg gagcgagcag gtccatcacc
     9781 tcggcgcgtc cgccgatcgc cgccagcggc atgccgccgc cgaccacctt gccgaaggtg
     9841 aacagatcgg gggcttcgtg cggtgaactg gtcgggctcg gcgtttcgga aggctcggcc
     9901 accgaggggc gctcgacagg cgaggaacgc tggacggtcg ggtccggggt ttcgaccgga
     9961 tcgaccagcg aggagtgctg ggcggtcgcg cccggggtct caataggctc gaccatcgag
    10021 gctctttcga ccgacgagga ccgctcggcc gccgcccgct ccaggcccca ccacccggac
    10081 g
//
LOCUS       DAOSUG010000031        10075 bp    DNA     linear   ENV 10-JUN-2024
DEFINITION  MAG TPA_asm: Micropruina sp. isolate AMR_MDS_2195
            NODE_3687_length_10075_cov_3.971956, whole genome shotgun sequence.
ACCESSION   DAOSUG010000031 DAOSUG010000000
VERSION     DAOSUG010000031.1
DBLINK      BioProject: PRJNA925477
            BioSample: SAMN32864262
            Sequence Read Archive: SRR4236673
KEYWORDS    WGS; ENV; Metagenome Assembled Genome; MAG; Third Party Data; TPA;
            TPA:assembly.
SOURCE      Micropruina sp. (wastewater metagenome)
  ORGANISM  Micropruina sp.
            Bacteria; Bacillati; Actinomycetota; Actinomycetes;
            Propionibacteriales; Nocardioidaceae; Micropruina.
REFERENCE   1  (bases 1 to 10075)
  AUTHORS   Abdulkadir,N., Saraiva,J.P., Zhang,J., Stolte,S., Gillor,O.,
            Harms,H. and Rocha,U.
  TITLE     Genome-centric analyses of 165 metagenomes show that mobile genetic
            elements are crucial for the transmission of antimicrobial
            resistance genes to pathogens in activated sludge and wastewater
  JOURNAL   Microbiol Spectr 12 (3), e0291823 (2024)
   PUBMED   38289113
REFERENCE   2  (bases 1 to 10075)
  AUTHORS   Abdulkadir,N.
  TITLE     Direct Submission
  JOURNAL   Submitted (24-MAR-2023) Environmental Microbiology, Helmholtz
            Center for Environmental Research -UFZ, Permoserstr 15, Leipzig
            04318, Germany
COMMENT     The annotation was added by the NCBI Prokaryotic Genome Annotation
            Pipeline (PGAP). Information about PGAP can be found here:
            https://www.ncbi.nlm.nih.gov/genome/annotation_prok/
            
            ##Genome-Assembly-Data-START##
            Assembly Method        :: SPAdes v. 3.11.1
            Genome Representation  :: Full
            Expected Final Version :: Yes
            Genome Coverage        :: Not Applicable
            Sequencing Technology  :: Illumina MiSeq
            ##Genome-Assembly-Data-END##
            
            ##Genome-Annotation-Data-START##
            Annotation Provider               :: NCBI
            Annotation Date                   :: 05/04/2023 04:57:10
            Annotation Pipeline               :: NCBI Prokaryotic Genome
                                                 Annotation Pipeline (PGAP)
            Annotation Method                 :: Best-placed reference protein
                                                 set; GeneMarkS-2+
            Annotation Software revision      :: 6.5
            Features Annotated                :: Gene; CDS; rRNA; tRNA; ncRNA
            Genes (total)                     :: 2,776
            CDSs (total)                      :: 2,750
            Genes (coding)                    :: 2,714
            CDSs (with protein)               :: 2,714
            Genes (RNA)                       :: 26
            tRNAs                             :: 24
            ncRNAs                            :: 2
            Pseudo Genes (total)              :: 36
            CDSs (without protein)            :: 36
            Pseudo Genes (ambiguous residues) :: 0 of 36
            Pseudo Genes (frameshifted)       :: 2 of 36
            Pseudo Genes (incomplete)         :: 32 of 36
            Pseudo Genes (internal stop)      :: 2 of 36
            ##Genome-Annotation-Data-END##
FEATURES             Location/Qualifiers
     source          1..10075
                     /organism="Micropruina sp."
                     /mol_type="genomic DNA"
                     /submitter_seqid="NODE_3687_length_10075_cov_3.971956"
                     /isolate="AMR_MDS_2195"
                     /isolation_source="Activated sludge of municipal
                     wastewater treatment plants"
                     /db_xref="taxon:2737536"
                     /environmental_sample
                     /geo_loc_name="Canada"
                     /lat_lon="49.25 N 123.1 W"
                     /collection_date="2015-06-16"
                     /metagenome_source="wastewater metagenome"
                     /note="metagenomic"
     gene            <1..855
                     /locus_tag="PLF56_01955"
     CDS             <1..855
                     /locus_tag="PLF56_01955"
                     /inference="COORDINATES: protein motif:HMM:NF012312.2"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_function: GO:0022857 - transmembrane transporter
                     activity [Evidence IEA];
                     GO_process: GO:0055085 - transmembrane transport [Evidence
                     IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="MFS transporter"
                     /protein_id="HOQ52357.1"
                     /translation="GTLLGAGTFALLGLANREVLFGWLWRVPFLLGAVLIVVAVVIRM
                     RLKESPAFEALEEAEAQRDAPVRQSVGQAIKTSRRNILVGIGLRMGENGNSSIYSALL
                     LAYVASLPAYKGQQLIGTWGAVVAALVAIFTVVAFGALSDKVGRVPVYRWGAFFTAAF
                     AFPGFYLLTLGQVWLVLLVMGVGVGIGVQSMLGPQCALLPELFGNAHRFTGVATAREF
                     SAVIAGGIAPLLGAVLLTATGNAWWVIAAYSAVLALISFATTFVTPETAGRSLTDFND
                     AERELVNA"
     gene            848..1906
                     /locus_tag="PLF56_01960"
     CDS             848..1906
                     /locus_tag="PLF56_01960"
                     /inference="COORDINATES: protein motif:HMM:NF014841.2"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="D-2-hydroxyacid dehydrogenase"
                     /protein_id="HOQ52358.1"
                     /translation="MLESNEKVRVVIAAPLAPELCDRIAAVDPRVEVVADQQLLPPMR
                     HAADFGCDPSWSRTPEQQNRYDALVDSAEILYGIPDVKPPALARTVAANPKLRWVMTM
                     AAGGGSQVVAAGLPADTFDHIVFTTSAGAHGVPLAEWALFGLFCGRKELPRLQRQQRA
                     HEWPTRIVGHPIEGSTVLIAGLGGIGQQVAKRCKALGMTVLGVKRQVSDVENVDEVHA
                     LADLDAIIGRADHIVLTLPGTAQTEKLLNAHLLGLAKPGVTVVNVGRGTVIDQNALID
                     ALRSGQVGFAALDVFEREPLEADSPLWDLENVLVSPHSGGLDSKEDERICEIFCENLK
                     LYLAGRPLHNVVDPNLGY"
     gene            1908..2999
                     /locus_tag="PLF56_01965"
     CDS             1908..2999
                     /locus_tag="PLF56_01965"
                     /inference="COORDINATES: ab initio
                     prediction:GeneMarkS-2+"
                     /note="Derived by automated computational analysis using
                     gene prediction method: GeneMarkS-2+."
                     /codon_start=1
                     /transl_table=11
                     /product="hypothetical protein"
                     /protein_id="HOQ52359.1"
                     /translation="MNLFVSANDAKLVRDFGRDVFPLDPGPDTLTVIDLDEARVLATV
                     PVQNSIVGPPQAVALSPDGSLAVVAAPTSYDRAAGVQRFADVLQVVQLRTEPPAVETI
                     PIGAHPQSVAFTPDGARLLATTVAGTVLLFDVADGAVELRQRLNLSAGSLSGLGVLAD
                     GSAAIVSLRDEQGAAVIDLTGPEAAVLPERVATGVSPYAVDTDASSRWAVISSVGWGG
                     KAAKAGRSVADADLITLVDTSRRPYRAVDHAAVPSIPEGVAISPDGGWVAALCMAGSQ
                     VAPGDAGHSPSGRLVLFANDNGRLRPVGELPTGAAAQGLAFADDSVRLVAQLYADRCL
                     AVYAVEHEHLVDTGTRIAVPGGPASLRRG"
     gene            3088..4338
                     /locus_tag="PLF56_01970"
     CDS             3088..4338
                     /locus_tag="PLF56_01970"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_015069381.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_component: GO:0016020 - membrane [Evidence IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="ArsB/NhaD family transporter"
                     /protein_id="HOQ52360.1"
                     /translation="MGEVISVAMLIVVLGFAVIRPRGLPEAVAAVPAALIVLLTGSLG
                     WSQAWAEIEHLFPVVAFLAAVLVISELCDADGLFRYAGALMAAKSQGNAHRLLIFVFV
                     LASAVTAVLSLDATVVLLTPVVFATASRMGVRPKPHAYACTHLANAGSLLLPVSNLTN
                     LLAMSAAGLTFVSFAGLMAAPWVAVIAVEYAAFRLFFASDLSIPATNPEPPEVPPLPR
                     FSAAVLALTLAGFVVASFFHIEAAWVALAGALVMLARALTLKQVSAKDVPGFINLGFL
                     GFVLSLGIVVTAVVEGGLGAAIGAIVPQGQSLVSLLLWAGLAAVLANIVNNLPAVLVL
                     LPFAAQAGTGAVLAVLIGVNVGPNLTYVGSLATLLWRRIVQAHDHETPIQEFTMLGLL
                     ATPLAIVVGVVALWGSLLVLGGAG"
     gene            4335..4820
                     /locus_tag="PLF56_01975"
     CDS             4335..4820
                     /locus_tag="PLF56_01975"
                     /inference="COORDINATES: protein motif:HMM:NF012791.2"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_function: GO:0032559 - adenyl ribonucleotide binding
                     [Evidence IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="universal stress protein"
                     /protein_id="HOQ52361.1"
                     /translation="MKLAVWVTEGTWPSCVDAANDLIGQQQAEITLIHVREGGRPAPG
                     PGFGSLMGSRRVRRAAHDLQELSQEAAQQLLDQAAERLGRVATVRLETGFPERVVTAL
                     ADEVDYLIVGRDGDRSRLGPHSLGRHTRFVIDHAPCRVLLIWPGAAPVIGSIPPPPPD
                     R"
     gene            5180..7252
                     /locus_tag="PLF56_01980"
     CDS             5180..7252
                     /locus_tag="PLF56_01980"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_015070618.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_process: GO:0009401 - phosphoenolpyruvate-dependent
                     sugar phosphotransferase system [Evidence IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="glucose PTS transporter subunit IIA"
                     /protein_id="HOQ52362.1"
                     /translation="MASTTAEQIVDAVGGPGNISSLTHCATRLRFELKDASVVDQKTV
                     ESIPGVLGAVPQSGDRYQVVIGGAVANTYADIMHLPVMAGAGKAAAGGSDADIKAAER
                     AKVRGKVAWLDAFFEYLSNSFRPLLGVLLGSSLIIAIAAVLDALHIVPFQGVDRMEAA
                     GATWVFWDSMWRSVLYFLPIMVAYNAAKALKVDPWIGGAVMAALFTPEFMSLSSAPGA
                     VCNTVFGQDQCVVQIFGLPMQLNGYGGQVFVPLIMVAVLAQLYKLMNRIFPENLQMVF
                     VPFFSLLIMVPVTAFLIGPLGVWIGGGLGTGLAWLNNTVPLLFAILIPMLYPFLVPLG
                     LHWPLNALMLANISSLGYDFIQGPMGTWNFACFGATAGVLVISSRVKDNEVRQTAIGA
                     LAAGLLGGISEPSLYGIHLRYKRIYPRMLVGCAVGGVTIGILGWIFGTVKTGAFAFTS
                     LLTIPVFNPMWVYAVSIAAAFATAMILVIISDYRTPEQKAEFEAEMAAAGGHLSVAEQ
                     NAYASASEAGVPAIPAAGVAAAETGTVGTLVLTRTVGAPVAGTVVPLAEVNDKVFASK
                     ALGDGVGIVPSDGHVTAPVAGTLVTVADTGHAFGIKTDDGVEVLVHVGIDTVQMEGKG
                     FNLDVAKDQRVEAGDLMATVDLEAIKAAGYDTTTIVVVINTATLSEVSPVAGGDVSNG
                     DPVINVVA"
     gene            7312..8148
                     /locus_tag="PLF56_01985"
     CDS             7312..8148
                     /locus_tag="PLF56_01985"
                     /inference="COORDINATES: protein motif:HMM:NF013070.2"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_process: GO:0006355 - regulation of DNA-templated
                     transcription [Evidence IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="PRD domain-containing protein"
                     /protein_id="HOQ52363.1"
                     /translation="MEILRVFNNNLVLALDEAGRDVILTGRGLGFQAKPGQPVDTAKV
                     VRTFVPIDGRDPDHLADMLAGIPPEHIHLVGQALAEIGLDSVSRNPAMVIALADHISF
                     ALRRITLGMTIVYPLLAEVKNLYAEEYAQAEALLRAVNARTDVQLPDLEAVGLALHLV
                     NAGFATGDLSYTYQMTGVIQQMVRVIEEAFDVTLDSGSVSVGRFITHLRYLFVRISQH
                     KQLDAEPSLVGAAIRESFPEASECAVRLAGLLELRLGADLTSDEVSYLALHVARVTTP
                     DR"
     gene            complement(8176..8529)
                     /locus_tag="PLF56_01990"
     CDS             complement(8176..8529)
                     /locus_tag="PLF56_01990"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_014845607.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="DUF488 family protein"
                     /protein_id="HOQ52364.1"
                     /translation="MTLKIKRVYEPAAPADGFRVLVDRLWPRGLSKERAAIDLWDKAV
                     APSSELRTAFHHNGMAWPDFEVAYRAEVAVNPAIAALRETLSQHPTATLLYSTHDDQH
                     NHAILLREALLHPER"
     gene            8648..9430
                     /locus_tag="PLF56_01995"
     CDS             8648..9430
                     /locus_tag="PLF56_01995"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_017934482.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_function: GO:0008168 - methyltransferase activity
                     [Evidence IEA];
                     GO_function: GO:0008757 - S-adenosylmethionine-dependent
                     methyltransferase activity [Evidence IEA];
                     GO_process: GO:0032259 - methylation [Evidence IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="class I SAM-dependent methyltransferase"
                     /protein_id="HOQ52365.1"
                     /translation="MTDTFDSLVEAGASADVSGWDFSWLDGRATEERPAWGYARQLAE
                     RLSRASASLDIQTGGGEVLAEAEAFPPAAVATESWPPNVAKATRLLHPHGVVVVADPD
                     EPPLPFADEAFDLVTSRHPATIWWQEIARVLRPGGTYFAQHVGPGSAFELIEFFLGPQ
                     PQARRGRHPDDETAAAEAAGLQIVNLQTAKLRIEIHDVAAAVYLLRKVIWWVPGFTVE
                     KYRDRLRDLHDLIGQDGPFIAHSTRHLIEARLRRGCGLVGIG"
     gene            complement(9618..9690)
                     /locus_tag="PLF56_02000"
     tRNA            complement(9618..9690)
                     /locus_tag="PLF56_02000"
                     /product="tRNA-Ala"
                     /inference="COORDINATES: profile:tRNAscan-SE:2.0.12"
                     /note="Derived by automated computational analysis using
                     gene prediction method: tRNAscan-SE."
                     /anticodon=(pos:complement(9655..9657),aa:Ala,seq:ggc)
     gene            9770..>10075
                     /locus_tag="PLF56_02005"
     CDS             9770..>10075
                     /locus_tag="PLF56_02005"
                     /inference="COORDINATES: protein motif:HMM:NF013491.2"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_function: GO:0005524 - ATP binding [Evidence IEA];
                     GO_function: GO:0016301 - kinase activity [Evidence IEA];
                     GO_process: GO:0016310 - phosphorylation [Evidence IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="PEP/pyruvate-binding domain-containing protein"
                     /protein_id="HOQ52366.1"
                     /translation="MTADTALVIPLSTCRAPDLDLVGGKGANLGELIAAGLPVPDGFV
                     ITTAAYDAVIGGAGVPALATSGDPDGLRTLFETIEVPPALVDQIRAGYAALGEPAVAV
                     "
BASE COUNT         1458 a         3494 c         3353 g         1770 t
ORIGIN      
        1 ggcaccctgc tgggtgccgg caccttcgcc ctgctcggcc tggccaaccg cgaggtgctg
       61 ttcggctggc tgtggcgggt gcccttcctg ctgggtgctg tgctcatcgt cgtcgccgtc
      121 gtgatccgca tgcgactcaa ggaatcgccc gcgttcgagg ctctcgaaga ggctgaggca
      181 cagcgcgacg cgccggtgcg gcagagcgtg ggccaggcca tcaagacgtc gcgccgcaac
      241 attctggtcg gcatcggcct gcgcatgggc gagaacggca acagctccat ctattccgca
      301 ctgctgctcg cctacgtggc cagcctgccc gcctacaagg gccaacagct gatcggcacc
      361 tggggtgcgg tcgtggcggc cctcgtcgcg atcttcaccg tcgtggcctt cggcgccctc
      421 tctgacaagg tcggacgcgt gcccgtctac cgctggggcg ccttcttcac cgccgcgttc
      481 gcctttcccg ggttctacct gctcaccctt ggtcaggtct ggctggtgct gctggtgatg
      541 ggcgtcggcg ttggtatcgg cgtgcaatcg atgctcggcc cccagtgcgc cctgctgccc
      601 gagctgttcg gcaacgcgca ccggttcacc ggcgtcgcca ccgcccgcga gttctcggcc
      661 gtgatcgccg gcggcatcgc acccctgctc ggggccgtgc tgctgaccgc gaccggcaac
      721 gcctggtggg tcatcgcggc ctactccgcg gttctcgcgc tgatctcgtt cgccaccacg
      781 ttcgtcaccc ccgagacggc aggccgtagc ctgacggact tcaacgacgc cgaacgggag
      841 ctcgtcaatg cttgagtcga atgagaaggt ccgggtggtc atcgccgccc cgctggcccc
      901 cgaactgtgc gaccgcatcg ccgccgtcga ccctcgggtc gaggtggtgg ctgaccagca
      961 gttgctgccc ccgatgcggc acgccgcgga cttcggctgc gacccgtcct ggtcacgcac
     1021 ccccgagcag cagaaccgct acgacgcgct cgtcgattcc gccgagatcc tgtacggcat
     1081 tccggacgtg aagccacccg cactggcgcg cacggtggcg gccaacccga aactgcgctg
     1141 ggtgatgacc atggcggccg gcggcggcag ccaggtggtg gccgccgggt tgcccgccga
     1201 caccttcgac cacatcgtgt tcaccacctc ggccggggcg catggcgtgc cgctggccga
     1261 gtgggcgctg ttcggcctgt tctgtggacg caaggaactc ccgcgcctgc aacgccagca
     1321 acgcgcccac gaatggccca cccgcatcgt cgggcacccg atcgagggca gcaccgtgct
     1381 gatcgccggc ctcggcggca tcggtcagca ggtcgccaaa cggtgcaagg cgctcggcat
     1441 gaccgtgctg ggcgtcaagc gccaggtgag cgacgtcgag aacgtcgacg aggtgcacgc
     1501 cctggccgat ctcgacgcga tcatcggccg ggccgaccac atcgtgctca cgctgccggg
     1561 caccgcccag accgagaagc tgctcaacgc gcacctgctg ggcctggcca aacccggtgt
     1621 gaccgtggtc aacgtgggcc gcggcaccgt gatcgaccag aacgccctga tcgacgcact
     1681 gcgctccggg caggtgggtt tcgccgctct ggacgtcttc gagcgcgaac cgctcgaggc
     1741 cgactccccg ctgtgggacc tggagaacgt gctggtcagc ccgcattcgg gcggcctcga
     1801 ttccaaagag gacgagcgca tctgcgagat cttctgcgag aacctgaagc tgtacctggc
     1861 cggccgtccc ctgcacaacg tggtcgaccc gaacctgggc tactgagatg aacctgttcg
     1921 tcagcgccaa cgacgccaag ctcgtccgcg acttcggccg tgacgtgttt ccgctcgacc
     1981 ccggccccga cacgctcacc gtgatcgatc tggacgaggc ccgcgtgctc gcgacagtgc
     2041 cggtgcagaa ctcgatcgtg gggcctccgc aggcggtggc gctctccccc gacggctctc
     2101 tggcggtcgt ggcggccccg accagctacg accgcgcggc gggcgtccag cggttcgcgg
     2161 acgtgctgca ggtcgtccag ttgcgcaccg agccccccgc ggtcgagacg attccgatcg
     2221 gcgcccaccc ccaatcggtc gccttcaccc ccgatggcgc gcgactgctc gccaccaccg
     2281 tggccggcac ggtgctgctc ttcgacgttg cggacggggc cgtcgagctg cgccagcggc
     2341 tgaacctcag cgccggctcc ctgtcgggtc tcggcgtgct cgccgacggt agcgcggcca
     2401 tcgtgtcgct gcgtgacgag cagggggccg cggtcatcga cctgaccggc ccggaggctg
     2461 ccgtgctgcc cgaacgcgtg gccaccggcg tcagcccgta cgcggtcgac accgacgcct
     2521 cgtcgcgctg ggcggtgatc agcagcgtcg gctggggcgg taaggcagcc aaagccggcc
     2581 gctcggtggc cgatgccgac ctgatcaccc tcgtcgacac ctcgcgccgt ccctaccgcg
     2641 ccgtcgatca cgccgccgtg ccgtccattc ccgagggcgt ggcgatctca cccgacggag
     2701 gttgggtggc cgccctgtgc atggccggca gccaggtcgc gcccggtgac gcgggccaca
     2761 gcccgtccgg acggttggtg ctgttcgcca atgacaacgg acgcctgcga cccgtgggcg
     2821 agttgcccac gggtgccgca gcgcagggtc ttgcgttcgc tgatgactcc gtgcggctgg
     2881 tggcgcagtt gtacgccgac cgctgtctgg cggtctacgc cgtcgagcac gagcatctgg
     2941 tcgacaccgg cacccgcatc gccgtgcccg gcgggccggc gtccctgcgg cgcggctgaa
     3001 ccaggcagcc acgcgtggtc ggggccctgt ccacccccct ttcggggccc tgacccaggt
     3061 tgttgcacta atctagggtg agcgcccgtg ggcgaggtga tctctgttgc gatgctgatc
     3121 gtggtgctcg gcttcgccgt gatcaggccc cgcgggctgc ccgaggcggt ggccgccgta
     3181 cccgccgcgc tgatcgtgct gctcaccggc tcgctcggct ggagccaggc ctgggccgag
     3241 atcgaacacc tgtttccggt ggtcgccttt ctggccgccg tgctggtgat cagtgagctc
     3301 tgcgacgcgg acgggctgtt ccgttacgcc ggcgccctga tggcggccaa gagccagggc
     3361 aatgcgcacc ggctgctgat cttcgtgttc gtgctggcct cggcggtcac cgccgtcctg
     3421 tcgctggatg ccaccgtggt gctgctgact ccggtggtgt tcgcgacggc gtccaggatg
     3481 ggggtgcggc cgaagccgca cgcgtacgcc tgcacccatc tggccaacgc cggctcgctg
     3541 ctgctgccgg tgtcgaacct gaccaacctg ttggcgatgt cggccgccgg gctcaccttc
     3601 gtcagcttcg ccgggctgat ggcggcgccc tgggtggcgg tgatcgccgt cgagtacgcg
     3661 gccttccgct tgttcttcgc ctccgatctc tccattccgg cgaccaaccc cgaaccgccc
     3721 gaggtgcccc cgctgccgag gttctcggcg gccgtgctgg cgctcacgct tgccggtttt
     3781 gtggtggcgt cgttctttca catcgaggcc gcctgggtgg cgctggcggg agccctggtg
     3841 atgctggccc gcgccctcac cctcaagcag gtgagcgcca aggacgtgcc cggcttcatc
     3901 aacctcggct ttctcggctt cgtgctctcg ctcgggatcg tggtgaccgc cgtggtcgag
     3961 ggcggtcttg gcgccgcgat cggagccatc gtgccgcagg gccagtcgct ggtctcgctc
     4021 ctgttgtggg cgggcctggc cgcggtgctg gccaacatcg tgaacaacct gcccgccgtg
     4081 ctggtgctgc tgcccttcgc cgcccaggcc ggcaccggcg ccgtgcttgc ggtgttgatc
     4141 ggcgtcaacg tgggcccgaa cctgacctac gtgggttcgc tggccaccct gctgtggcgg
     4201 cggatcgtcc aggcgcacga ccacgagacg ccgatccagg agttcaccat gctcggtctg
     4261 ctggccacac ccctcgccat cgtcgtgggc gtggtggcac tctggggctc actgctcgtg
     4321 ctgggaggtg ccggatgaag ctcgcggtgt gggtgaccga gggcacctgg ccctcatgcg
     4381 tggacgccgc gaacgacctg atcggacagc agcaggccga gatcaccctg atccacgtgc
     4441 gcgagggcgg acggcccgcg cccgggcccg gcttcgggtc actgatgggt agcaggcggg
     4501 tgcgccgggc cgcgcacgat ctgcaggaac tgtcgcagga ggcggcgcaa caactgctcg
     4561 atcaggccgc cgagcgactg ggtcgggtgg ccaccgtccg gctcgagacc ggctttcccg
     4621 agcgggtggt gaccgccctg gccgacgagg tcgactacct gatcgtcgga cgagacggcg
     4681 accgcagccg gctgggcccc cacagcctgg gcaggcacac ccggttcgtg atcgaccatg
     4741 cgccgtgccg tgtgctgctc atctggcccg gcgccgcgcc cgtgatcggt tcgatcccgc
     4801 cgccgccacc ggatcgctga gctgcctacc gagcacgtcg acaccacacc cgcccgggga
     4861 gcttgtcgag ccgccgcgtt gcccggaggt ctcgacccgc tcgaccaacg atgcgattgg
     4921 acacattcga tcgagcgagc gtgtctgtgt gctccataca ggtagtagca ccacggtttg
     4981 atttctcggc gtgtcgggtg tgacccaggg ggcacacgac cacacccctg gtgctagtgt
     5041 gctgattgag caaaccgctc aacagaaggg ttgttacccg gcaggggcaa aacctgagct
     5101 gcaccagggt gcaactcggg tctttttttg tctctgcccc taatgccccc atacgttgct
     5161 agtagatagg aacacctcaa tggcgtccac tactgccgaa cagatcgtcg acgcagtcgg
     5221 cggtcccggc aatatctcga gcctgaccca ctgcgccacc cggcttcgct tcgaactgaa
     5281 ggatgccagc gtggtcgacc agaagactgt cgaatccatt cccggcgtgc tcggtgccgt
     5341 accgcagagc ggcgatcggt accaggtggt gatcggcggc gcggtcgcca acacctacgc
     5401 cgacatcatg cacctgcccg tgatggccgg tgccggtaag gcggcggccg gcggcagcga
     5461 tgccgacatc aaggccgccg aacgcgccaa ggtccgcggc aaggtcgcct ggctcgacgc
     5521 gttcttcgag tacctctcga actcgttccg gccgctgctc ggcgtgctgc tcggctcgtc
     5581 gctgatcatc gccatcgccg ccgtgctgga cgccctgcac atcgtgccct tccagggcgt
     5641 cgatcgcatg gaggcagccg gtgccacctg ggtgttctgg gacagcatgt ggcgttcggt
     5701 gctgtacttc ctgccgatca tggtcgccta caacgccgcc aaggcgctca aggtcgaccc
     5761 gtggatcggc ggtgcagtca tggctgcgct gttcacgccc gagttcatgt cgctgagttc
     5821 agcgccgggt gcggtctgca acacggtctt cggccaggac cagtgcgtcg tccagatctt
     5881 cggcctgccg atgcaactca acggctacgg cggccaggtc ttcgtgccgc tgatcatggt
     5941 cgccgtgctg gcccagctct acaagctgat gaaccggatc ttccccgaga acctgcagat
     6001 ggttttcgtg ccgttcttca gcctgctgat catggttccg gtgaccgcgt tcctgatcgg
     6061 cccgctcggt gtctggatcg gcggcggcct cggtaccgga ctggcctggc tgaacaacac
     6121 cgttccgctg ctgttcgcga tcctgattcc gatgctgtac ccgttcctgg tgccgctggg
     6181 cctgcactgg ccgctcaatg cgctcatgct ggccaacatc agcagcctgg gctacgactt
     6241 catccagggc ccgatgggca cctggaactt cgcctgcttc ggtgccaccg ctggcgtgct
     6301 ggtgatctcg agtcgggtca aggacaacga ggtgcggcag accgccatcg gcgcgctggc
     6361 cgcaggcctg ctgggtggta tctcggagcc gtcgctgtac ggcatccacc tgcgctacaa
     6421 gcgcatctac ccgcgcatgc tggtcggctg tgccgtcggt ggcgtgacga tcggcatcct
     6481 gggctggatc ttcggcacgg tgaagaccgg cgccttcgcc ttcacctcgc tgctgaccat
     6541 tccggtgttc aatccgatgt gggtgtacgc ggtctcgatc gcggccgcct tcgccaccgc
     6601 aatgatcctg gtcatcatct ctgactaccg cacccccgag cagaaggccg agttcgaggc
     6661 cgagatggct gccgccggcg gtcacctgag cgtcgcggag cagaatgcct acgcgagcgc
     6721 gtccgaggcc ggcgttcccg ccattcccgc cgccggtgtt gcggcagctg agaccggcac
     6781 ggtcggcacc ctggtgctca cccgcaccgt cggcgccccg gtggccggca ccgtcgtccc
     6841 gttggccgag gtcaacgaca aggtgttcgc ctccaaggca ctgggtgacg gggtcggcat
     6901 cgtgcccagc gacggccatg tgacggcccc ggtggccggc accctggtca ccgtcgccga
     6961 caccggccac gccttcggta tcaagaccga cgacggcgtc gaggtgctgg tgcacgtcgg
     7021 catcgacacg gtgcagatgg agggcaaggg gttcaacctc gacgtcgcga aggatcagcg
     7081 cgtggaggcc ggcgacctga tggcgaccgt cgacctggag gccatcaagg cagccgggta
     7141 cgacaccacc acgatcgtgg tcgtgatcaa caccgcgacc ctgtccgagg tctcgcccgt
     7201 cgccggtggt gacgtcagca acggtgaccc ggtgatcaac gtggtcgcct gaccaacaac
     7261 caaagtccgg ggcggctcag tacactgagc cgccccggct tttgtgagca catggaaatc
     7321 cttcgcgtct tcaacaacaa cctcgtcctg gcgctggacg aggccggtcg cgacgtcatt
     7381 ctgaccggtc gcggcctggg ctttcaggcc aaacctggcc agccggtcga caccgccaag
     7441 gtggtgcgca cgttcgtacc catcgatgga cgcgaccccg accatctggc cgacatgctg
     7501 gccggcattc cccctgaaca cattcacctg gtcggtcagg cccttgccga gatcggtctc
     7561 gactcggtca gccgcaaccc cgcaatggtg atcgcgctgg ccgatcacat cagcttcgcg
     7621 ttgcgccgca tcaccctcgg catgaccatc gtgtacccgc tgctggccga ggtgaagaat
     7681 ctctacgccg aggaatacgc gcaggccgag gccctgctgc gcgcggtcaa cgcgcgcacc
     7741 gacgtgcagc tacccgacct cgaggcggtc ggcctggcgc tgcacctggt gaacgccgga
     7801 ttcgccaccg gcgatctgtc ctacacctac cagatgaccg gtgtgatcca gcagatggtg
     7861 cgggtgatcg aagaggcctt cgacgtcacc ctcgactctg gcagcgtcag cgtcggccgt
     7921 ttcatcaccc acctgcggta cctgttcgtc cgcatctcgc agcacaagca actggacgcc
     7981 gaaccgtccc tggtcggtgc cgcgattcgg gagtcgtttc ccgaggcctc cgagtgtgcc
     8041 gtccggttgg ccggactgct cgaactgcgg ctgggcgccg acctcacctc ggacgaggtg
     8101 tcgtacctgg cccttcacgt cgcgcgggtg accacaccgg atcgctgagc ccggtgatga
     8161 tccgttcgcc gcggatcatc gttcggggtg cagcagcgct tcgcgcagca gtatcgcgtg
     8221 gttgtgttga tcgtcgtgcg tcgaatagag cagggtcgcg gtcgggtgct gcgagagcgt
     8281 ctcgcgcagc gccgcgatcg cggggttcac ggcgacctca gcgcggtagg ccacctcgaa
     8341 gtcgggccag gccatgccgt tgtggtgaaa cgccgtccgc agctcggacg agggcgccac
     8401 ggccttgtcc cacaggtcga tcgcggcccg ctctttcgac agtccgcgcg gccacagccg
     8461 gtcgacgagc actcggaagc cgtcggcagg cgccgccggc tcatagacac gcttgatctt
     8521 cagggtcata cccacagtca acgccggatg ctcgctgcgc accagggctg ggagggtcgt
     8581 ggggtcgcat gttcgtcgta gatgcgacgc catcattccg gcaacgctgt ctgggagagt
     8641 gtgcctcatg actgacacgt tcgactcact ggtcgaggcc ggagcttccg ccgacgtgtc
     8701 cgggtgggac ttctcctggc tggacggacg agcaaccgag gaacgccccg cctggggcta
     8761 tgcccggcag ctcgccgagc ggctcagccg ggcatcggcg tcactggata tccagaccgg
     8821 cggcggcgag gttctcgccg aagccgaggc cttccctccc gccgctgtcg cgacggagtc
     8881 gtggccgccg aacgtcgcga aggccacccg actgttgcat cctcacggtg ttgtcgtcgt
     8941 ggcagacccg gacgagcctc cgttgccgtt tgctgacgag gccttcgatc tggtcaccag
     9001 caggcatcct gcgacgattt ggtggcaaga gatcgcccgt gtgctccggc ccggcggcac
     9061 ctacttcgcc cagcacgtcg gacccggcag tgcattcgag ctgatcgagt tctttctcgg
     9121 cccgcaaccc caagccaggc gaggacgcca ccctgacgac gagacagccg cagcagaagc
     9181 cgccgggttg cagatcgtca atctgcaaac agcgaagctg cggatcgaga tccatgatgt
     9241 tgcggccgcg gtgtacctgc tgcggaaggt gatctggtgg gtgccagggt tcacggtgga
     9301 gaagtaccga gatcggctcc gtgacctgca cgacctcatc gggcaggacg gaccgttcat
     9361 cgcccactcc actcgtcacc tgatcgaggc caggctaagg cgagggtgtg ggctcgtcgg
     9421 aatcggatga tgcggggtgt tcccgatggt ccacgtcgca gcgagcttca ttgccgaatc
     9481 tgatgagcta gctgtcggcg ttgatgtatg ccgcacgggc cgtactaagt tctgccccac
     9541 gcgcctcgtg gccgccaagg gttgggctga ccccgacacg ccttcgcatg tcacgggttc
     9601 gagtacgcca accacggtgg agctaagggg attcgaaccc ctgacctctt ccatgccatg
     9661 gaagcgcgct accaactgcg ccatagcccc gtgccttgcg gacgagggtt tacgttaccg
     9721 gatcgccgcg accttgtgaa atcaggaacg ccggagaaga gtggcggtca tgacggctga
     9781 tacggccctg gtgatccccc tgtccacctg ccgggcccca gacctcgacc tggtcggcgg
     9841 caagggtgcg aacctgggcg agttgatcgc cgcgggcctt ccggtaccgg acggcttcgt
     9901 gatcaccacc gccgcctatg acgcagtgat cggcggcgcc ggcgtgccgg ccctggccac
     9961 gagcggcgac cccgacggcc tccggacgct gttcgagacg atcgaggttc cccccgcact
    10021 ggtcgaccag atcagggccg gctacgcggc cctgggcgag ccggccgtcg ccgtg
//
LOCUS       DAOSUG010000032        10052 bp    DNA     linear   ENV 10-JUN-2024
DEFINITION  MAG TPA_asm: Micropruina sp. isolate AMR_MDS_2195
            NODE_3695_length_10052_cov_4.189057, whole genome shotgun sequence.
ACCESSION   DAOSUG010000032 DAOSUG010000000
VERSION     DAOSUG010000032.1
DBLINK      BioProject: PRJNA925477
            BioSample: SAMN32864262
            Sequence Read Archive: SRR4236673
KEYWORDS    WGS; ENV; Metagenome Assembled Genome; MAG; Third Party Data; TPA;
            TPA:assembly.
SOURCE      Micropruina sp. (wastewater metagenome)
  ORGANISM  Micropruina sp.
            Bacteria; Bacillati; Actinomycetota; Actinomycetes;
            Propionibacteriales; Nocardioidaceae; Micropruina.
REFERENCE   1  (bases 1 to 10052)
  AUTHORS   Abdulkadir,N., Saraiva,J.P., Zhang,J., Stolte,S., Gillor,O.,
            Harms,H. and Rocha,U.
  TITLE     Genome-centric analyses of 165 metagenomes show that mobile genetic
            elements are crucial for the transmission of antimicrobial
            resistance genes to pathogens in activated sludge and wastewater
  JOURNAL   Microbiol Spectr 12 (3), e0291823 (2024)
   PUBMED   38289113
REFERENCE   2  (bases 1 to 10052)
  AUTHORS   Abdulkadir,N.
  TITLE     Direct Submission
  JOURNAL   Submitted (24-MAR-2023) Environmental Microbiology, Helmholtz
            Center for Environmental Research -UFZ, Permoserstr 15, Leipzig
            04318, Germany
COMMENT     The annotation was added by the NCBI Prokaryotic Genome Annotation
            Pipeline (PGAP). Information about PGAP can be found here:
            https://www.ncbi.nlm.nih.gov/genome/annotation_prok/
            
            ##Genome-Assembly-Data-START##
            Assembly Method        :: SPAdes v. 3.11.1
            Genome Representation  :: Full
            Expected Final Version :: Yes
            Genome Coverage        :: Not Applicable
            Sequencing Technology  :: Illumina MiSeq
            ##Genome-Assembly-Data-END##
            
            ##Genome-Annotation-Data-START##
            Annotation Provider               :: NCBI
            Annotation Date                   :: 05/04/2023 04:57:10
            Annotation Pipeline               :: NCBI Prokaryotic Genome
                                                 Annotation Pipeline (PGAP)
            Annotation Method                 :: Best-placed reference protein
                                                 set; GeneMarkS-2+
            Annotation Software revision      :: 6.5
            Features Annotated                :: Gene; CDS; rRNA; tRNA; ncRNA
            Genes (total)                     :: 2,776
            CDSs (total)                      :: 2,750
            Genes (coding)                    :: 2,714
            CDSs (with protein)               :: 2,714
            Genes (RNA)                       :: 26
            tRNAs                             :: 24
            ncRNAs                            :: 2
            Pseudo Genes (total)              :: 36
            CDSs (without protein)            :: 36
            Pseudo Genes (ambiguous residues) :: 0 of 36
            Pseudo Genes (frameshifted)       :: 2 of 36
            Pseudo Genes (incomplete)         :: 32 of 36
            Pseudo Genes (internal stop)      :: 2 of 36
            ##Genome-Annotation-Data-END##
FEATURES             Location/Qualifiers
     source          1..10052
                     /organism="Micropruina sp."
                     /mol_type="genomic DNA"
                     /submitter_seqid="NODE_3695_length_10052_cov_4.189057"
                     /isolate="AMR_MDS_2195"
                     /isolation_source="Activated sludge of municipal
                     wastewater treatment plants"
                     /db_xref="taxon:2737536"
                     /environmental_sample
                     /geo_loc_name="Canada"
                     /lat_lon="49.25 N 123.1 W"
                     /collection_date="2015-06-16"
                     /metagenome_source="wastewater metagenome"
                     /note="metagenomic"
     gene            complement(<1..427)
                     /locus_tag="PLF56_02010"
     CDS             complement(<1..427)
                     /locus_tag="PLF56_02010"
                     /inference="COORDINATES: protein motif:HMM:NF013328.2"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_function: GO:0003906 - DNA-(apurinic or apyrimidinic
                     site) endonuclease activity [Evidence IEA];
                     GO_function: GO:0008270 - zinc ion binding [Evidence IEA];
                     GO_function: GO:0019104 - DNA N-glycosylase activity
                     [Evidence IEA];
                     GO_process: GO:0006284 - base-excision repair [Evidence
                     IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="DNA-formamidopyrimidine glycosylase family
                     protein"
                     /protein_id="HOQ52367.1"
                     /translation="MPEGDTVWRTARRLHQVFAGEPLTRCDLRWPELATIDFSEQRTL
                     EVASRGKHLLHRLDSGWTIHSHLRMDGQWRIEPPDTRANANTRALLATSQWTALGQRL
                     GMLDVVRTADEHTLVGHLGPDVLGPDWNPAVAAGNIAAAG"
     gene            complement(449..5092)
                     /locus_tag="PLF56_02015"
     CDS             complement(449..5092)
                     /locus_tag="PLF56_02015"
                     /EC_number="3.6.4.-"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_020576472.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_function: GO:0003676 - nucleic acid binding [Evidence
                     IEA];
                     GO_function: GO:0005524 - ATP binding [Evidence IEA];
                     GO_function: GO:0016818 - hydrolase activity, acting on
                     acid anhydrides, in phosphorus-containing anhydrides
                     [Evidence IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="ATP-dependent helicase"
                     /protein_id="HOQ52368.1"
                     /translation="MTDDVLTRFSPATAAWFRGSFSAPTAAQTGAWTSISSGQHTLVV
                     APTGSGKTLSAFLWALDRLASEPPPEKLKRCRVLYVSPLKALAVDVERNLRSPLVGIG
                     HEAVRLGLPAPDITVAVRSGDTPADQRRTFAREPSDILITTPESLFLLLTSAAREALA
                     GVETVIIDEIHALAGGKRGAHLALSLERLDALLPKPAQRIGLSATVRPIDEVARFLAG
                     ARPVSIVNPPSEKSWDLSVVVPVPDLGALGELTDDLSGPAAGAQQRTSIWPHVEEKIV
                     DLIAAHRSTLVFANSRRLAERLTARLNEIWAERTAEPGEPDEADGSNPLDQRHAPAQI
                     MAQAGQSHGAPLLLARAHHGSVSKEQRAATEEDLKAGRLPAVVATSSLELGIDMGAVD
                     LVVQVEAPPSVASGLQRVGRAGHQVGATSRGVLFPKFRGDLVQTAVITERMRAGAIEE
                     LRVPSNPLDVLAQQIVAICALDDITVTDLEALVRRAAPFSGLTRGVLESVLDMLAGRY
                     PSEAFAELRPRLVWDRGTDTLTGRRGAQRLAVTSGGTIPDRGLFGVFIVGEGEGRRVG
                     ELDEEMVYESRVGDVITLGTSTWRIEEITHDQVRVSPAPGEAGRLPFWKGDTLGRPAE
                     LGRALGGFVRELGAMAPEAALQRARTAGLDDWAADNLVTYLAEQREAIPQLPSDTTIV
                     VERFRDELGDWRVVVHSPFGAPVHAPWALAIAARLREAHGVDVQAMHGDDGIVLRLPE
                     TDWSEAETAAPDLLETIVIDPDEVAGIVTEQIGGSALFASRFRECAGRALLLPKRRPG
                     QRQALWQQRQRASQLLEVASQYPSFPIVLEAVRECVQDVFDVPALVELMRQIGNRRVR
                     TVEVTTSRPSPFASSLLFGYVAQFLYEGDSPLAEKRAAALTLDPTLLSELLGTSDGLA
                     LADLLDPESLTRTETELQHLAEGRRARDAEDLADVIRVLGPLDLAALQARSATPDATA
                     GWLVTLSEQRRVIEVRVAGRTHWAAVEDTGRLIDALGVSVPVGVPEVHRAPVPDPLGD
                     LVVRWARNHAPFLAGEVAAWLGLGTAVVHGVLRRLVGEGRLVEGDLRPPGWQPPAHTT
                     QPAGPAATHDPAENHQRSGGLDYCDPGVLRVLRRRSLAALRNAVEPVPPRQFARFLTH
                     WQGIAQPLRGTAGLLAAVQQLAGYPVPASSLESLVLPARVRDYTPAMLDELLAAGDVV
                     WQGAGALAGTDGWVTLHPAETAPLTLAAPSDVADELERRVLEALAGGGSYLFRALSQR
                     LGIGDTELHAALWQLVWSGHLSNDTLAPLRALLGGGRATLKQRPTAARLRYGRRPLAR
                     GPLPTASGPPTTTGRWSLLPTVETDPTVLAAARADVLLDRYGVVTRGSVANEEVTGGF
                     AGAYRVFAALEESGQVRRGYFVEGLGAAQFGDRHAVDLLREAGEVPGAVVLAATDPAN
                     PYGAALGWPARAEGTRHQPGRKAGALVVLVDGDLALYVERGGRTLLSFTPDPTALTSA
                     ASALAQAVHAGALGKLTVAKIDGADVLGSDHALADALAAAGFRLTPQGLRLRR"
     gene            complement(5348..5560)
                     /locus_tag="PLF56_02020"
     CDS             complement(5348..5560)
                     /locus_tag="PLF56_02020"
                     /inference="COORDINATES: ab initio
                     prediction:GeneMarkS-2+"
                     /note="Derived by automated computational analysis using
                     gene prediction method: GeneMarkS-2+."
                     /codon_start=1
                     /transl_table=11
                     /product="hypothetical protein"
                     /protein_id="HOQ52369.1"
                     /translation="MLAAFANRVLPFDLPAARVLATYRVPEHAPLDDALIAAVAEAAG
                     LTVVTRSIKHFEPTGVACLNPWEFTP"
     gene            complement(5765..5989)
                     /locus_tag="PLF56_02025"
     CDS             complement(5765..5989)
                     /locus_tag="PLF56_02025"
                     /inference="COORDINATES: ab initio
                     prediction:GeneMarkS-2+"
                     /note="Derived by automated computational analysis using
                     gene prediction method: GeneMarkS-2+."
                     /codon_start=1
                     /transl_table=11
                     /product="toxin-antitoxin system"
                     /protein_id="HOQ52370.1"
                     /translation="MEQILIRNLPDGTKAALRARSERHHRSVEAEARALLTESLTRDN
                     VTILALLSTDEGADIEFEPERLGLKARSAE"
     gene            complement(6102..8558)
                     /locus_tag="PLF56_02030"
     CDS             complement(6102..8558)
                     /locus_tag="PLF56_02030"
                     /inference="COORDINATES: protein motif:HMM:NF013126.2"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_function: GO:0004553 - hydrolase activity, hydrolyzing
                     O-glycosyl compounds [Evidence IEA];
                     GO_process: GO:0005975 - carbohydrate metabolic process
                     [Evidence IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="glycoside hydrolase family 3 N-terminal
                     domain-containing protein"
                     /protein_id="HOQ52371.1"
                     /translation="MMLGAWPAPPTASGQPKECSCDPFPTLAAIVSGLLTATMLTPVG
                     AAEARPAGASYTERDISDGVTTFTVVTNPGDGATLSFVKGGPVKLLTVRSGRTTLAFK
                     DLNANGRLDAWEDWRRPVAERAKALAQELTMEQIAGLMLFSSHERAPEAGLTDAQRKY
                     LKDDHLRNVLNAGANDVTANVTWVNAVQAFAEQQISATNPYVPVNFSSDPRSTAANDS
                     AYNAEGDDISRWPSNLGLAATFSRSTMLNFAKASSAEYRAMGITTALGPQIDLATEPR
                     WLRVDGTFGENVELAKTMAKAYVDGSQNTYDKRGRSLGWGSDSINTMIKHWPGDGAGE
                     GGRESHLNAGKYAVYPGDNFDEHTEPFLAAKNATAVMSSYSIAIAKDGSPLTGNRVGS
                     AYDKVKIDLLRDSGYDGVICTDWGVTTGYTDANSIFGMAWGMEKASVNERHYAVLRAG
                     VDMFGGNNKTAPVLAAHALWQADFKAGKNAIDADTRFRQSAERILRMLMLPGLFENPY
                     LDLAASKKIVASDDKIQAGLDAQFDSVVMLKNANRTIRPSSLKKFAKQTVYIPSSIGH
                     GFPSVFSQATTDITGPTLDAEVAKKYFKKVLTDTPVLDSAGKVIDYVMPDLKDVDVVL
                     AGMRSPDNGNNFTGAGRNSDGTYYPLSLQWGPYTANGPNVRRTSIAGDIRDDGTVENR
                     SYFGASSRIGNAYDLTALKRTVKAVKGIERRTGRNIPVIVAVKAKTSFIPAEFEKKVD
                     AILVGYSLNDKVLIETALGQHEPQGRLPIASPKNMNAVEKQLEDVGEDMKPYVDSVGH
                     KYLFGYGLNWRGVIGERRHR"
     gene            8742..9362
                     /locus_tag="PLF56_02035"
     CDS             8742..9362
                     /locus_tag="PLF56_02035"
                     /inference="COORDINATES: ab initio
                     prediction:GeneMarkS-2+"
                     /note="Derived by automated computational analysis using
                     gene prediction method: GeneMarkS-2+."
                     /codon_start=1
                     /transl_table=11
                     /product="hypothetical protein"
                     /protein_id="HOQ52372.1"
                     /translation="MPHTLLHRVSIRPRHLDAWLATWPEQVRLREKHGFTHLQAYVQT
                     HAEPKLTWLYAHDDPAAGEAALAADPAAFALREQLVPHVFGNLVVRPVRIELLENRSD
                     EPGRTVCLRRYSIVGEWTGFLAIWRQIVQVRERYGFRCRFAVADEPKDLFTWAFDFEG
                     TWDDFAAVQRPYYRDPERVALRKVFDYMADYTLDPAVPLRPAGLSG"
     gene            complement(9352..9861)
                     /locus_tag="PLF56_02040"
     CDS             complement(9352..9861)
                     /locus_tag="PLF56_02040"
                     /inference="COORDINATES: ab initio
                     prediction:GeneMarkS-2+"
                     /note="Derived by automated computational analysis using
                     gene prediction method: GeneMarkS-2+."
                     /codon_start=1
                     /transl_table=11
                     /product="hypothetical protein"
                     /protein_id="HOQ52373.1"
                     /translation="MPPLRLASIGLLLVLVTINLGSFTLSLDLLGWLMVVGGMHQLAE
                     VNQWFGWARNLAVAALVLALVLMISPFAMINVLASPLSRLAVLAFVFCLCTALLQRLG
                     RGDRGYAITLQVLRVLIPLVTVAAAVFATIDAPAGLPVEWLYPVQLIGTAVLAVVMWA
                     LGSRYSFSR"
BASE COUNT         1506 a         3483 c         3567 g         1496 t
ORIGIN      
        1 gcccggcggc ggcgatgttg cccgcggcca ccgccgggtt ccagtcgggg ccgagtacgt
       61 ccggccccag gtggccgacc agggtgtgct catcggcggt gcgcaccacg tctagcatgc
      121 ccagtcgctg gcccagcgcg gtccattgac tggtggcgag cagggcgcgg gtgttggcgt
      181 tcgcccgggt gtccgggggc tcgatgcgcc actggccgtc catacgcagg tggctgtgga
      241 tcgtccagcc agaatcgagg cgatgcagca ggtgcttgcc gcggctggcc acctcgagcg
      301 tgcgctgctc gctgaagtcg atggtggcga gttcgggcca gcgcaggtcg caacgggtca
      361 gcggctcgcc ggcgaacacc tggtgcaggc ggcgggcggt acgccagacg gtgtcaccct
      421 cgggcatggg tgttcttcgg ccgcagggtc atcggcgcaa cctcagcccc tgcggggtca
      481 gccggaaccc ggcggcggcc agggcgtcgg ccaacgcgtg gtcgctgccc agcacgtcgg
      541 cgccgtcgat cttggcgacg gtgagcttgc ccaacgcacc ggcgtggacg gcctgcgcga
      601 gggccgaggc ggcggaagtc agggcggtgg ggtctggagt gaacgagagc agggtgcgtc
      661 cgccacgttc gacgtacagc gcgaggtcgc cgtcgaccag caccaccagg gcgccggcct
      721 tgcgtccggg ttgatgcctg gtgccctcgg cgcgggccgg ccagcccagg gccgcgccgt
      781 aggggttggc cgggtcggtg gcggccagca cgaccgcgcc gggcacctcg cccgcctcac
      841 gcagcaggtc gaccgcgtgc cggtcgccga actgggcggc acccaagccc tcgacgaagt
      901 agccacgccg cacctggccc gactcctcca gcgccgcgaa cacccggtag gcgccggcga
      961 atccgccggt gacctcttcg ttggccacac tgccgcgggt gaccacgccg taacggtcga
     1021 gcagcacgtc ggcgcgggcg gcagcgagca ccgtcggatc ggtctcgacg gtgggcagca
     1081 atgaccagcg tccggtggtc gtgggcggtc cggacgcggt gggcaacggg ccccgggcca
     1141 gcggacggcg tccgtagcga agccgcgctg cggtcggacg ctgcttcagg gtggcccggc
     1201 cgcccccgag cagggcacgc agcggcgcga gggtgtcgtt gctgaggtgc cccgaccaga
     1261 ccagctgcca tagcgccgcg tgcagttcgg tgtcaccgat cccgaggcgc tgcgacaagg
     1321 cacggaacag gtagctgccg ccaccggcca gcgcctccag cacccggcgc tccagctcgt
     1381 cggccacgtc ggacggtgcc gccagcgtca gcggcgcggt ctcggccggg tgcagggtga
     1441 cccagccgtc ggtgccggcc agcgccccgg cgccctgcca gaccacgtca ccggcggcca
     1501 gcagttcgtc cagcatcgcg ggggtgtagt cgcgcacccg ggcgggcagc accagagatt
     1561 ccagtgagct ggcgggcacc ggatagccgg cgagttgttg gacggccgcg agcagccctg
     1621 cggtgccgcg caggggctgg gcgatgccct gccagtgggt gagaaagcgg gcgaactgcc
     1681 gcggcggcac tggttcgacg gcgttgcgca gggcggccag cgaccggcgg cgcagcaccc
     1741 gcagcacgcc ggggtcgcag tagtcgagcc cgccggagcg ctggtggttc tcggcggggt
     1801 cgtgggtggc ggcgggcccg gccggttgag tcgtgtgcgc cggcggctgc caaccgggcg
     1861 gacgcaggtc gccctcgacc agccggcctt caccgaccag ccgacgcagc accccgtgca
     1921 cgaccgccgt cccgagcccc agccaggcgg cgacctcacc ggcgaggaag ggtgcgtggt
     1981 tgcgggccca gcgcaccacc aggtcgccca gcgggtcggg gaccggcgcc cggtgcacct
     2041 cgggcacccc gaccgggacg ctgaccccga gggcgtcgat cagccgtccg gtgtcttcga
     2101 ccgcagccca atgggtgcgc cccgccaccc gcacctcgat cacccggcgc tgctcgctca
     2161 gcgtgaccag ccagccggcc gtggcatcgg gtgtggcgct gcgggcctgc agtgccgcca
     2221 gatcgagggg acccagcacc cggatcacgt cggccaggtc ttcggcgtcg cgtgcccgcc
     2281 gcccctcggc cagatgctgc aactcggtct cggtgcgggt cagcgattca gggtcgagca
     2341 ggtcggccag ggcgaggccg tccgaggtgc cgagcaactc cgacagcagg gtggggtcga
     2401 gagtgagcgc ggccgcccgc ttctcggcca gcggcgagtc gccctcgtac aggaactgcg
     2461 cgacgtagcc gaacagcaac gacgacgcga acggcgaggg gcgactcgtg gtcacttcga
     2521 cggtacgcac ccgccggttg ccgatctgcc gcatgagttc gaccagggcc ggcacgtcga
     2581 acacgtcctg cacgcattcg cgcaccgctt cgagcacgat cggaaaactg gggtactgcg
     2641 acgccacctc aagcagttgc gaggcccgtt gccgctgctg ccacaaggcc tgacgttggc
     2701 ccggacgacg cttgggcagc aacagcgcac ggccggcgca ctcgcggaac cggctggcga
     2761 acaacgccga gccgccgatc tgctcggtga cgatgccggc cacctcgtcg ggatcgatca
     2821 cgatggtctc gagcaggtcg ggggccgcag tttcggcctc ggaccagtcg gtctcgggca
     2881 gccgcagcac gatgccgtcg tcgccgtgca tggcctgcac gtcgaccccg tgcgcctcgc
     2941 gcagccgggc ggcgatggcc agcgcccacg gcgcgtgtac cggcgcgccg aacggtgagt
     3001 ggacgaccac ccgccagtca ccgagttcgt cgcggaagcg ttccaccacg atcgtggtgt
     3061 cgctgggcag ttgcggaatc gcctcgcgct gctcggccag gtaggtgacc aggttgtcgg
     3121 ccgcccagtc gtccaggccc gccgtgcgcg cccgctgcag tgccgcctcg ggggccatcg
     3181 cccccaactc gcggacgaac ccgcccagcg cacgccccag ctcggccggc cggcccaggg
     3241 tgtcgccctt ccagaacggc agccggcccg cctcgcccgg ggccgggctg acccgcacct
     3301 ggtcgtgggt gatctcttcg atgcgccagg tggacgtgcc gagggtgatc acgtcgccga
     3361 cccgtgattc gtagaccatc tcttcgtcga gctcgccgac ccggcgaccc tcaccttcgc
     3421 cgacgatgaa caccccgaac aggccgcgat cgggaatggt gccgcccgag gtgacggcca
     3481 gccgctgggc gccgcggcgt ccggtgaggg tgtcggtgcc gcggtcccag accagcctgg
     3541 gccgcagttc ggcgaacgcc tcggacgggt agcgtccggc cagcatgtcg agcaccgact
     3601 cgagcacacc gcgggtgagg ccgctgaagg gggccgcgcg gcggaccagc gcctcgaggt
     3661 cggtcacggt gatgtcgtcg agggcgcaga tggccacgat ctgctgggcc agcacgtcga
     3721 gcggattgga cggcacccgc aactcttcga tcgcaccggc ccgcatgcgc tcggtgatca
     3781 cggcggtctg caccaggtcg ccgcggaact tggggaacag cacgcctcgc gaggtggcac
     3841 ccacctggtg gccggcgcgg ccgacccgct gcagtcccga cgcgaccgac ggcggcgcct
     3901 ccacctgcac caccaggtcg accgcgccca tgtcgatgcc gagttccagg gacgaggtgg
     3961 ccacgacggc cgggaggcgt ccggccttga ggtcttcttc ggtggcggcc cgctgctctt
     4021 tgctgacgga gccgtggtgg gcgcgggcca gcaggagcgg ggcgccgtgc gattggccgg
     4081 cctgggccat gatctgcgca ggcgcatgcc gttggtcgag tgggttgctg ccgtcggcct
     4141 catctggctc gcccggctcg gccgtccgct cggcccagat ctcgttcagc cgggcggtca
     4201 gccgctcggc cagccgacgc gagttggcga acaccagagt ggaacggtgc gccgcgatca
     4261 ggtcgacgat cttctcttcg acgtgcggcc agatcgaggt gcgctgctgc gccccggctg
     4321 cggggccgga caggtcgtcg gtcagctcac ccaatgcgcc caggtcgggc accggcacca
     4381 ccaccgacag gtcccatgac ttctcgctgg gcgggttgac gatgctgacc ggacgcgcgc
     4441 cggccaggaa ccgcgccacc tcgtcgatcg gacgcaccgt ggccgacaac ccgatgcgtt
     4501 gcgccggctt tggcagcagg gcgtccaagc gctcgagcga cagcgccaga tgtgcgcccc
     4561 gcttgccgcc ggccagcgcg tggatctcgt cgatgatgac cgtctcgacc ccggccagcg
     4621 cctcgcgggc agcagaggtg agtagcagga acagcgactc gggcgtggtg atcagaatgt
     4681 cggacggctc gcgcgcgaac gtgcggcgct ggtcggccgg ggtgtcgccc gagcgcacgg
     4741 cgacggtgat gtcgggtgcc ggcaggccca gccgcaccgc ttcgtgcccg atgccgacca
     4801 ggggtgagcg caggttgcgt tccacatcga cggccagcgc cttcagaggc gacacgtaca
     4861 gcacccggca gcgtttcagc ttctcaggcg gcggttcgct ggccagccga tcgagcgccc
     4921 acagaaacgc cgacagcgtc ttgcccgacc cggtgggcgc caccaccagc gtgtgctgac
     4981 ccgaactgat cgaggtccag gcccccgtct gggcggccgt gggcgcggag aagctgcccc
     5041 ggaaccaggc tgcggtggcc ggggagaacc gcgtcagcac gtcgtcggtc atatcgggca
     5101 ccacaatgcc acgggcctct gacagttccg ctggtcgagt cgagaccgtc gatcagcgaa
     5161 tctgcctgac gttgccgccg gaaccccagg accggtcagc cgtccaagcc gcaacgccca
     5221 gccggtgggt ggtcgccaga cagaagcgat ctgccagcga caggtgcgga ttgctgcgca
     5281 agcacaaaac acccgcccga ccaggccgag ggcgcggtca ggcaggtgat cgatcagatg
     5341 gagcgcctca cggcgtgaac tcccacgggt tcaggcacgc gaccccggtc ggctcgaagt
     5401 gtttgatgct gcgtgtcacc acggtcaggc cggcggcttc ggcgactgcg gcgatcaggg
     5461 cgtcatccaa gggtgcgtgc tcgggaaccc ggtaggtcgc cagaactcgg gccgccggca
     5521 ggtcgaaggg cagcacgcgg ttggcgaatg cagcgagcac gttctgctcg ccagcgacgc
     5581 agcaccgccc cctgcgctgg atcagtgcgc tccgtgacgg tcacgcctcg ctcaatctcg
     5641 gcgacggtga acgcggcgat gcacaggtct tcgaagggga tcgacgccgc ccacgcctcg
     5701 acggcgggat tacgcccgcg cactcgcagc gccgacacca cgttggtgtc caacaggtac
     5761 ctcattattc ggcggagcgc gccttcaggc ccagccgctc gggctcgaac tcgatgtcgg
     5821 caccttcgtc agtactcagc agggcaagga tcgtgacgtt gtcgcgggtc agggactcgg
     5881 tgagcagtgc gcgcgcttcc gcctcgacgg agcggtgatg ccgctcggaa cgggcgcgca
     5941 gcgcggcctt ggttccgtcc ggcaagttgc ggatcaggat ctgttccatg caacctcctt
     6001 gatatcaatc gatgatatca cttcgagcgc ggccgacgcc ttcggcaccg cgatcccgcc
     6061 ggccgactga cggtcagccg acggaatcgc gaccgatcgc ctcagcggtg ccggcgctcg
     6121 ccgatcaccc cgcgccagtt caggccgtag ccgaacaggt acttatgccc caccgaatcg
     6181 acgtacggct tcatgtcctc accgacgtcc tcgagctgct tctcgacagc gttcatgttc
     6241 ttcgggctgg cgatcggcag tcgtccctgc ggttcgtgct ggcccagagc cgtctcgatc
     6301 agcaccttgt cgttgagcga gtagccgacc aggatcgcgt ccaccttctt ctcgaactcg
     6361 gccggaatga agctggtctt ggccttcacc gcgacaatca ccggaatgtt gcgcccggtg
     6421 cgccgctcga tgcccttgac cgccttcacc gttcgcttca gcgcggtcag atcgtaggca
     6481 ttgccgatgc gggagctggc cccgaagtac gaccggttct cgaccgtgcc gtcgtcacga
     6541 atatcgccgg cgatggacgt gcggcgcacg ttcggaccgt tggcggtgta cggcccccac
     6601 tgcagcgaca acgggtagta ggtgccgtcg gagttgcgtc cggcgccggt gaagttgttg
     6661 ccgttgtcgg ggctgcgcat gccggccagc accacgtcga cgtccttcag gtcgggcatc
     6721 acgtagtcga tcaccttgcc ggcgctgtcg agcaccgggg tgtcggtgag caccttcttg
     6781 aagtacttct tggccacctc tgcgtcgagc gtcggcccgg tgatgtcggt ggtggcttgg
     6841 gagaacacgc tcgggaagcc gtgcccgatc gaactcggaa tgtagacggt ctgcttggcg
     6901 aacttcttca acgaggacgg ccggatcgtg cggttcgcgt tcttcagcat caccacggag
     6961 tcgaactggg cgtccaggcc ggcctggatc ttgtcgtcgc tggcgacgat cttcttggac
     7021 gcggccaggt cgaggtaggg gttctcgaac agaccgggca gcatcagcat gcgcaggatg
     7081 cgttcggccg actggcggaa ccgggtgtcg gcgtcgatgg cgttcttgcc ggccttgaag
     7141 tcggcctgcc acagggcgtg ggcggccagc accggcgcgg tcttgttgtt gccgccgaac
     7201 atgtcgacgc cggcccgcag caccgcgtag tgccgctcgt tgaccgaagc cttctccatg
     7261 ccccacgcca tgccgaagat cgagttggcg tcggtgtagc cggtggtgac accccagtcg
     7321 gtgcagatca cgccgtcgta gccggagtcg cgcagcaggt cgatcttgac cttgtcgtag
     7381 gcggaaccga cccggttgcc ggtcagcggc gagccgtcct tggcgatcgc gatggagtac
     7441 gaactcatca ccgcggtggc attcttggcc gccaggaacg gctcggtgtg ctcgtcgaag
     7501 ttgtcacccg ggtacacggc gtacttgccg gcgttcaggt gcgactcgcg gccaccctcg
     7561 ccggcaccgt cgcccggcca gtgcttgatc atcgtgttga tcgagtccga gccccagccg
     7621 agtgagcgtc cgcgcttgtc gtaggtgttc tgagagccgt ccacgtaggc cttggccatc
     7681 gtcttggcca actcgacgtt ctcaccgaac gtgccgtcga cccgcagcca gcgaggctcg
     7741 gtggccagat cgatctgcgg gccgagcgcg gtggtgatgc ccatcgcccg gtattcggcc
     7801 gaggacgcct tggcgaagtt cagcatcgtc gagcgcgaga aggtggccgc caggccgagg
     7861 ttggacggcc agcgcgagat gtcgtcgccc tcggcgttgt aggccgagtc gttggcagcc
     7921 gtggagcgcg ggtcggagct gaagttgacc ggcacgtagg ggttggtggc cgagatctgc
     7981 tgctcggcga aggcctgcac ggcgttcacc caggtcacgt tcgcggtgac gtcgttggcg
     8041 ccggcgttca gcacgttgcg caggtggtcg tccttgaggt atttgcgctg cgcgtcggtc
     8101 aggccggcct cgggcgcacg ctcgtgcgag ctgaacagca tcaagccggc gatctgctcc
     8161 atggtcagct cctgcgcgag cgccttggcc cgctcggcga ccggacgacg ccagtcctcc
     8221 caggcgtcga ggcgaccgtt ggcgttgagg tccttgaacg ccagggtggt gcggcccgag
     8281 cgcaccgtca gcagcttcac cgggccgccc ttgacgaagc tcagcgtggc accgtcgccg
     8341 gggttggtca ccactgtgaa tgtggtgacg ccgtcactga tgtcacgctc ggtgtagctt
     8401 gcgccggccg ggcgggcctc ggcggccccc acaggggtca gcatggtggc ggtgagcagg
     8461 ccgctgacga tcgcggccag ggtggggaat gggtcgcatg aacactcctt gggctgcccg
     8521 ctggcggtcg ggggtgcggg ccaggcgccg agcatcattg cccgagcgtc tggccgtcat
     8581 gctcgcacat catccccgtg gggcaccacc actcgacgac ccggggatcg aggccccatg
     8641 acgggccacg tgacccggcc tcgacgttca ggaccggcag cgacccgcgg cgaccggcag
     8701 cgaccggcgg cacccggcgg cgaccggcgt aggctgcccg cgtgccccac accctgctgc
     8761 accgcgtctc gattcgaccc cgccacctgg acgcctggct ggccacctgg cccgagcagg
     8821 tgcggctgcg tgagaagcac ggtttcaccc acctgcaggc ctacgtgcag acccacgccg
     8881 agccgaagct gacctggctc tacgcccacg acgacccggc agccggcgag gctgccctgg
     8941 cggctgatcc cgcggccttc gccctccgtg agcaactcgt gccgcacgtg ttcggcaacc
     9001 tcgtcgtgcg ccccgtccgg atcgaactgc tggagaaccg ttccgacgag ccgggccgca
     9061 ccgtgtgcct gcgccgctac tcgatcgtcg gcgaatggac gggcttcctg gcgatctggc
     9121 gacagatcgt ccaggtgcgg gaacgttacg gcttccgctg ccggttcgcg gtagccgacg
     9181 aaccgaaaga cctgttcacc tgggcgttcg acttcgaggg aacgtgggac gacttcgcgg
     9241 ccgtgcagcg gccctactat cgcgaccccg aacgggtcgc gctgcgcaag gtcttcgact
     9301 acatggccga ctacaccctc gaccccgcgg tgccgttgcg cccggcagga ctcagcggct
     9361 gaacgagtac cgcgaaccca acgcccacat gaccacggcg agcacggccg tgccgatcag
     9421 ctggacgggg tagagccact ccaccggcaa cccggccggg gcatcgatgg tcgcgaacac
     9481 ggctgcggcc accgtgacca gcggaatcag cacccgcaac acctgcaggg tgatcgcgta
     9541 gccgcggtcg ccgcgaccca gtcgctgcag cagggcggtg cacaggcaga acacgaacgc
     9601 cagcacggcc agccggctga gcggcgaggc gagcacattg atcatcgcga acggcgagat
     9661 catcagcacc agcgccagca cgagtgccgc caccgccagg ttgcgggccc acccgaacca
     9721 ctggttcacc tcggccagtt gatgcatgcc gccgaccacc atcagccagc cgagcaggtc
     9781 gagcgacagc gtgaatgaac ccaggttgat ggtgaccagc acgagcagca ggccgatcga
     9841 ggccaggcga aggggaggca tgcgccaagg gtaaccggac gccgccgtac ccggcccagc
     9901 cggacgggta ccggtggact ggagcagcag gtgcacccgg tttcgacgag ctcgaacagg
     9961 cgatggtctc gacgagctcg aacggcgtgg tctctaccta cggccggggg atcacccgcc
    10021 gcacatccgg cctgaccgat ggaccccgct cg
//
LOCUS       DAOSUG010000033        10011 bp    DNA     linear   ENV 10-JUN-2024
DEFINITION  MAG TPA_asm: Micropruina sp. isolate AMR_MDS_2195
            NODE_3710_length_10011_cov_4.743070, whole genome shotgun sequence.
ACCESSION   DAOSUG010000033 DAOSUG010000000
VERSION     DAOSUG010000033.1
DBLINK      BioProject: PRJNA925477
            BioSample: SAMN32864262
            Sequence Read Archive: SRR4236673
KEYWORDS    WGS; ENV; Metagenome Assembled Genome; MAG; Third Party Data; TPA;
            TPA:assembly.
SOURCE      Micropruina sp. (wastewater metagenome)
  ORGANISM  Micropruina sp.
            Bacteria; Bacillati; Actinomycetota; Actinomycetes;
            Propionibacteriales; Nocardioidaceae; Micropruina.
REFERENCE   1  (bases 1 to 10011)
  AUTHORS   Abdulkadir,N., Saraiva,J.P., Zhang,J., Stolte,S., Gillor,O.,
            Harms,H. and Rocha,U.
  TITLE     Genome-centric analyses of 165 metagenomes show that mobile genetic
            elements are crucial for the transmission of antimicrobial
            resistance genes to pathogens in activated sludge and wastewater
  JOURNAL   Microbiol Spectr 12 (3), e0291823 (2024)
   PUBMED   38289113
REFERENCE   2  (bases 1 to 10011)
  AUTHORS   Abdulkadir,N.
  TITLE     Direct Submission
  JOURNAL   Submitted (24-MAR-2023) Environmental Microbiology, Helmholtz
            Center for Environmental Research -UFZ, Permoserstr 15, Leipzig
            04318, Germany
COMMENT     The annotation was added by the NCBI Prokaryotic Genome Annotation
            Pipeline (PGAP). Information about PGAP can be found here:
            https://www.ncbi.nlm.nih.gov/genome/annotation_prok/
            
            ##Genome-Assembly-Data-START##
            Assembly Method        :: SPAdes v. 3.11.1
            Genome Representation  :: Full
            Expected Final Version :: Yes
            Genome Coverage        :: Not Applicable
            Sequencing Technology  :: Illumina MiSeq
            ##Genome-Assembly-Data-END##
            
            ##Genome-Annotation-Data-START##
            Annotation Provider               :: NCBI
            Annotation Date                   :: 05/04/2023 04:57:10
            Annotation Pipeline               :: NCBI Prokaryotic Genome
                                                 Annotation Pipeline (PGAP)
            Annotation Method                 :: Best-placed reference protein
                                                 set; GeneMarkS-2+
            Annotation Software revision      :: 6.5
            Features Annotated                :: Gene; CDS; rRNA; tRNA; ncRNA
            Genes (total)                     :: 2,776
            CDSs (total)                      :: 2,750
            Genes (coding)                    :: 2,714
            CDSs (with protein)               :: 2,714
            Genes (RNA)                       :: 26
            tRNAs                             :: 24
            ncRNAs                            :: 2
            Pseudo Genes (total)              :: 36
            CDSs (without protein)            :: 36
            Pseudo Genes (ambiguous residues) :: 0 of 36
            Pseudo Genes (frameshifted)       :: 2 of 36
            Pseudo Genes (incomplete)         :: 32 of 36
            Pseudo Genes (internal stop)      :: 2 of 36
            ##Genome-Annotation-Data-END##
FEATURES             Location/Qualifiers
     source          1..10011
                     /organism="Micropruina sp."
                     /mol_type="genomic DNA"
                     /submitter_seqid="NODE_3710_length_10011_cov_4.743070"
                     /isolate="AMR_MDS_2195"
                     /isolation_source="Activated sludge of municipal
                     wastewater treatment plants"
                     /db_xref="taxon:2737536"
                     /environmental_sample
                     /geo_loc_name="Canada"
                     /lat_lon="49.25 N 123.1 W"
                     /collection_date="2015-06-16"
                     /metagenome_source="wastewater metagenome"
                     /note="metagenomic"
     gene            complement(<1..1542)
                     /locus_tag="PLF56_02045"
     CDS             complement(<1..1542)
                     /locus_tag="PLF56_02045"
                     /EC_number="3.4.24.-"
                     /inference="COORDINATES: protein motif:HMM:NF013591.2"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_function: GO:0004222 - metalloendopeptidase activity
                     [Evidence IEA];
                     GO_function: GO:0008270 - zinc ion binding [Evidence IEA];
                     GO_process: GO:0006508 - proteolysis [Evidence IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="M3 family metallopeptidase"
                     /protein_id="HOQ52374.1"
                     /translation="MTFDAANPFASPSTLPYRLTPWAQVRAEHYLPAVDAGIEQRLAE
                     LQAVADHPEPPDQANVIDAWERSGQLLGRALGAFWTVQPADTTDELDAIDAEIAPKLA
                     RLGDAIYQNRALYDRVTALAERVEAGEVQLDPQASYWLETRLRDFVRAGVGLPEADQA
                     RLRELNATLAELQSAFGRRALAASNAAAVLVTDEAELAGASDSSKAGARAAAETRGVD
                     GWLLELDSCTPQAILTELSDRDLRRRVHEASTGRGWSGDYDTRELVVEIARARAERAV
                     LLGYHSHSAYVAEEACAKTSEAVHAMLSRLAAPAVRNARAEAADLQPLQEEPLEPWDW
                     EYRSEQLKGKRYHFDAALLRPYLELERVLHEGVFAAATGLFGLTFTERTDLAGYNDDV
                     RVFEVFDADGTGLGLFLADYYTRAGKQGGAWMNNIVDQNHLLGQQPVVVNNLNIVKPP
                     AGEPTLLTWDEVRTLFHEFGHALHGLLSDVRYPSQSGTNTPRDFVEFPSQVNEMWALD
                     AGLLRR"
     gene            complement(1639..1770)
                     /locus_tag="PLF56_02050"
     CDS             complement(1639..1770)
                     /locus_tag="PLF56_02050"
                     /inference="COORDINATES: ab initio
                     prediction:GeneMarkS-2+"
                     /note="Derived by automated computational analysis using
                     gene prediction method: GeneMarkS-2+."
                     /codon_start=1
                     /transl_table=11
                     /product="hypothetical protein"
                     /protein_id="HOQ52375.1"
                     /translation="MYAPIAAIEAALRQNTSALPTALQAHEAPIVDPWDANPLLVLR"
     gene            complement(2355..2678)
                     /locus_tag="PLF56_02055"
     CDS             complement(2355..2678)
                     /locus_tag="PLF56_02055"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_007079349.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="rhodanese-like domain-containing protein"
                     /protein_id="HOQ52376.1"
                     /translation="MSVPEIAVTDLPDDAVVLDVREPGEWARGHVPNAVHLPMGEVSD
                     QLANLPKLDGPLPVVCRSGGRSAKVVEFLRVQGVDAVNVTGGTQAWAAAGKPLAADEG
                     EPTVI"
     gene            complement(2726..3112)
                     /locus_tag="PLF56_02060"
     CDS             complement(2726..3112)
                     /locus_tag="PLF56_02060"
                     /inference="COORDINATES: ab initio
                     prediction:GeneMarkS-2+"
                     /note="Derived by automated computational analysis using
                     gene prediction method: GeneMarkS-2+."
                     /codon_start=1
                     /transl_table=11
                     /product="hypothetical protein"
                     /protein_id="HOQ52377.1"
                     /translation="MSSKSPLSSHTRITPEKVGRAATKVAAEAAFVLTGMADVVAGTV
                     QDVVKQGRADYTERKAAGEAPIASQVRQVPGRVRGLVDEVKDAYRTLSARGRTVLSEG
                     FSSTAHRDQPTGQRAAPGDYEQPPTN"
     gene            3332..4657
                     /locus_tag="PLF56_02065"
     CDS             3332..4657
                     /locus_tag="PLF56_02065"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_011757745.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="dipeptidase"
                     /protein_id="HOQ52378.1"
                     /translation="MTLHDAVKAVLPQALLDLAELVAIPSVSALPAHAGDVQRSAEWI
                     ADKLRALHCPDVRIVAEGGAPAVIGRFPAPPGAPTICLYAHHDVQPTGELAEWTGDPF
                     VVHEADGRLFARGAGDDKGGVLVHLAALRAFEGRPPVGVTVFVEGEEEIGSPSLADII
                     ARHHDELAADVYVIADSGNWQVGRPAFTDTLRGLVDCVVEVSTLDHALHSGQFGGAVP
                     DALTTLCRLLATLHDDQGNVAIEGLSAMPDPTLDYPKERLLTETGLLEGVHLIGDGSI
                     AGRLWTKPTATVLAIDANSVAEASNILAPTARAKVSVRIAPDADPAAALSAVTEHLRR
                     NAPWGAHVNVIDGSTGSGTTVAFTGPAAEAARAAFVEAWNVEPVFIGQGGSIPMVADF
                     QQAFPDATVLVTAVCDPDSRMHGIDESLNLDDFAKACLAETLLLAKLAP"
     gene            complement(4844..6265)
                     /locus_tag="PLF56_02070"
     CDS             complement(4844..6265)
                     /locus_tag="PLF56_02070"
                     /EC_number="2.7.11.1"
                     /inference="COORDINATES: protein motif:HMM:NF012298.2"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_function: GO:0004674 - protein serine/threonine kinase
                     activity [Evidence IEA];
                     GO_function: GO:0005524 - ATP binding [Evidence IEA];
                     GO_process: GO:0006468 - protein phosphorylation [Evidence
                     IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="serine/threonine-protein kinase"
                     /protein_id="HOQ52379.1"
                     /translation="MTEQLLAARYRLEREIGAGGMGRVWRARDIRLDREVAIKTVDLS
                     GVRDPSLAGRFQREIVLTARLTSPYTVTVFDGGVDGSTAYLVMELLRGESLAELIARR
                     GPLGLTATLDVARQVTLGLAAAHAVGLVHRDIKPANVMLTEGGRVKLLDFGIAQLTGQ
                     ADPHLTAPSMTIGTAAYMSPEQAAGLRVDAATDLYALGCLIMTMMTGRPPFDGDQPVV
                     VAGHQINSAPPRLNQRRPDVPQGLDALVAGLLAKTPLERPSTAATLRALEHLQGNPSA
                     ATVPVAVPPPTPPPTAAPMQPTRPYTQPAPAQSAASHGVRGPAHLPQQPGSWWIAGVL
                     LSLMAAVLVAGLAVNLSGLALTNLGRAFPSASASARPSSASPKPSVTASKKKTTTPDP
                     GHAVALAAALQGVQLALNSLPDSAAKSDLGQRWSTTSSRIAAGTNPKDNLAAFEKAVR
                     ASDLQGWERQAVLTATGIVKSLL"
     gene            complement(6266..6970)
                     /locus_tag="PLF56_02075"
     CDS             complement(6266..6970)
                     /locus_tag="PLF56_02075"
                     /inference="COORDINATES: ab initio
                     prediction:GeneMarkS-2+"
                     /note="Derived by automated computational analysis using
                     gene prediction method: GeneMarkS-2+."
                     /codon_start=1
                     /transl_table=11
                     /product="hypothetical protein"
                     /protein_id="HOQ52380.1"
                     /translation="MALSLRALGAVGVLALLTACGTTPPVGVPSSPVAPATSSGPSTE
                     PSPSPSASDSPSATARSSSPSVSPTASASPSASAVLVLQPKGVDQFAFGTAEATVTAA
                     LNAKAGKADESYSGKVCEVDGDTPYGRQLLYGAGVFLFQSKARGTKNSPRTFTSWVVN
                     LDQPLGSTLRLADGYPVDTTFAKLKTAFPKGKLRAITLGESSVWVFTTPSGLWYRGDD
                     NKTPTDVGAGAMGTCE"
     gene            complement(7026..8375)
                     /locus_tag="PLF56_02080"
     CDS             complement(7026..8375)
                     /locus_tag="PLF56_02080"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_013863092.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_function: GO:0016811 - hydrolase activity, acting on
                     carbon-nitrogen (but not peptide) bonds, in linear amides
                     [Evidence IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="acetamidase/formamidase family protein"
                     /protein_id="HOQ52381.1"
                     /translation="MTNQSTQPDPAQAARILESGVGRRGLLLAAAAVGAGGTLAAATS
                     ATEAAAAPRGFYEGVLQPGKGRIRGDHYLSSRTGEVLWGYVPTVHAKPVLTMRSGRTV
                     TIDSVSHEGILEDQGRDPVAWLGEHGVRRREILDDAIDIAANYRRTTRSFDVDGPHVV
                     TGPIRVAGAEPGDVLKIETLDVRARVPYGVVSSRHGKGALAITSAGTAPAGITLDEVM
                     PPHDTDGRSSGIPTDYHNVSVFTALEGGKAVMRSGRRRLRFPVNPFFGMMGVAYSQAS
                     GLTDPSANSIPPTLGGGNIDIRHLGVGSTFYLPVAAEGALFYVGDPHHAMGDGEVALT
                     AMEGSLRGTFRLTVCKKGSGTAPSVAFRYPFAETASSWIPIGLSDPDGSVNGQGNDLN
                     VAMRRAVVNALDFLQQDQGLNRAVAYAYLSAAANFSVSQVVDRTTGVHALIRKSDLH"
     gene            8583..>10011
                     /locus_tag="PLF56_02085"
     CDS             8583..>10011
                     /locus_tag="PLF56_02085"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_015071730.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_function: GO:0003824 - catalytic activity [Evidence
                     IEA];
                     GO_process: GO:0005975 - carbohydrate metabolic process
                     [Evidence IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="alpha-amylase family glycosyl hydrolase"
                     /protein_id="HOQ52382.1"
                     /translation="MSPKKSATPADEPASGKQAATTEAKPEAKGAGKSPKAGKNAKPT
                     GKSSATGKGSTAGKDSTAGRGPKAGKTAKPTGKDSTGKSSTGKGSTSRNAKQPGRGAK
                     GAATRPSVNQQPALEGMGAIVTAEGCGFRVWAPHAESVAVTGTFNDWSTTSDPLGGEG
                     NGFWYGFVPGAKAADEYKFALTNGEQSLERIDPYAYQVTNSVGNGIVYDHAAFDWQND
                     SFSCPAHNELVIYELHVGSFAPKTEGEVGTFDEVQASIEHFQRLGISAIQLMPSAEFA
                     GDYSWGYNPAHIFAVESAYGGPDALKTLVREAHRAGIAVIMDVVYNHFGPTDLDLWQF
                     DGWSENDKGGIYFYNDWRSSTPWGDTRPDYGRGEVRSYIRDNAEMWLRDYHVDGLRFD
                     MTPYMRSVNAGTLDLPDGWSLQSWINRWIREQFPDAITIAEDLHGEPLVTSTAPEGAA
                     YHAQWEAKFVHPVREAIIVASDAYRS"
BASE COUNT         1520 a         3405 c         3545 g         1541 t
ORIGIN      
        1 gcgccgcagc agaccggcgt ccagcgccca catctcgttc acctgggacg ggaactcgac
       61 gaagtcccgc ggcgtgttgg tgcccgattg cgacgggtag cggacgtcgg agagcagccc
      121 gtgcagcgcg tgcccgaact cgtggaacag ggtgcgcacc tcgtcccagg tcagcagggt
      181 gggctcaccg gccggcggct tgacgatgtt gaggttgttc accaccaccg gctgctggcc
      241 gagcaggtgg ttctggtcga cgatgttgtt catccaggcc ccgccctgct tgccggcgcg
      301 ggtgtagtag tcggccagga acagtcccag cccggtaccg tccgcgtcga acacctcgaa
      361 gacgcgaacg tcgtcgttgt agccggccag gtcggtgcgt tcggtgaagg tgaggccgaa
      421 cagccccgtg gctgcggcga acacgccctc gtgcagcacc cgctccagtt cgaggtaggg
      481 ccgcagtagg gcggcgtcga agtggtagcg cttgcccttg agctgctcgg agcggtactc
      541 ccagtcccag ggctcgagcg gctcctcctg caggggttgg aggtcggcgg cctcggcgcg
      601 ggcgttgcgc accgcgggtg cggcgagccg gctgagcatc gcgtgcaccg cctcggacgt
      661 cttcgcgcac gcctcttcgg ccacgtacgc ggagtggctg tgatagccga gcaggaccgc
      721 ccgctcggcg cgggcacgag cgatctcgac caccagttcg cgggtgtcgt agtcaccgct
      781 ccagccccgt ccggtggacg cctcgtgcac ccgccggcgc aggtcgcggt cgctgagttc
      841 ggtcaggatc gcctgcggcg tgcatgaatc caactcgagc aaccagccgt ccacgccgcg
      901 ggtctcggcg gcagcgcggg cccccgcctt ggacgagtcg gacgctccgg ccagctcggc
      961 ctcgtccgtg accagcacgg cggccgcgtt cgaggcggcc agcgcccgcc gtccgaacgc
     1021 cgactgcagc tcggccaagg tggcgttcag ttcgcgcagc cgcgcctgat ccgcctcggg
     1081 caggcccacc ccggcccgga cgaagtcgcg cagccgggtc tccagccagt aggacgcctg
     1141 cgggtcgagt tgcacctcgc cggcctcgac ccgctcggcc agcgccgtca cccggtcgta
     1201 gagcgcgcgg ttctggtaga tcgcgtcgcc cagcctggcg agcttggggg cgatctcggc
     1261 gtcgatggcg tccaactcgt cggtggtgtc ggccggctga acggtccaga aggcgcccag
     1321 agcccgtccg agcagttgtc cggaccgctc ccaggcgtcg atcacgttgg cctggtcggg
     1381 gggttcgggg tgatcggcga cggcctgcag ttcggccagc cgctgctcga tgccggcatc
     1441 gacggcaggc aggtagtgct cggcgcgcac ctgggcccac ggagtgagcc ggtacggcag
     1501 cgtggacggg ctggcgaacg ggttcgcggc atcgaacgtc atggcttcat cttgcccagt
     1561 cgccgcggtt gtgcgcacct gaggtgcatc cggccaccgc cgacgcgggt ctgactcact
     1621 gtcctggacg tgctgcgatc agcgcagcac cagcagcggg ttggcgtccc aggggtcgac
     1681 gatgggtgcc tcgtgcgcct gcagcgcggt cggcaacgcc gaggtgttct ggcgcaatgc
     1741 ggcctcgatg gcggcgatcg gtgcgtacat ggtgattctc cttctttcgg cgcaaggcac
     1801 agctcacgcc aacggttgta tcgaaatggt tgtgactagg tgttgtactg gacggcccga
     1861 cgctatgccg acagcgagcg atcaggcggg gcggacctgg cgagcagtgc cctcgggttc
     1921 gagcggtaac tcgcccgcga gccggagcgc cgagccaccc gcctcggcgg cgtctcgtgc
     1981 ggttcgactc atcggtctcc ctgacatcta ttatgatctg taacgttaaa gaacttaact
     2041 ggccgggatt ctttaccgtt aaagtcgtcg ctgagggttg tcctgccagc gagcccagtc
     2101 aggccatcag gatgatcgcg ggctaccccg gccggggtag caaagccacc acgcgagcag
     2161 ccagatcggt ccgcggtagg cagcggaatc gcgacgaaga gatcggtggg ctgcatcaac
     2221 ccgtgacgtg aggccgttgt ccgcaggtgg aatcacggcc ggtggagccg gcgggcgggc
     2281 cgtgttcgcg tgcgcgatcg tccctcggtg cgttgttgac ggtcgatgac cgggggaggg
     2341 cgctcagctg gtcgtcagat gaccgtcggt tcgccttcgt cggcggccaa cggcttgccg
     2401 gccgccgccc acgcctgggt gccgccggtc acgttgacgg cgtccacccc ctggacccgc
     2461 agaaactcga ccaccttcgc cgatcgcccg ccggaacggc agaccaccgg cagcggcccg
     2521 tccagcttcg gcaggttggc gagctggtcg ctcacctcac ccatcggcag gtggacagcg
     2581 ttcggcacgt gcccgcgggc ccattcgccc ggttctcgca cgtcgagcac cacggcatca
     2641 tcgggcaggt cggtcacggc aatctctggc acgctcaccg atccatactg acacggtttg
     2701 ccccgggcat catcgtcgtg agcgatcagt tggtcggcgg ctgctcgtag tcgccgggcg
     2761 ccgcccgctg cccggtcggc tggtcgcgat gcgcggtgga cgagaagccc tcgctgagca
     2821 ccgtgcggcc ccgcgcggac agcgtccggt aggcgtcctt gacctcatcg accaggcccc
     2881 ggacgcgtcc gggcacctgc ctgacctgac tcgcgatcgg ggcttcaccg gccgccttcc
     2941 gctcggtgta atcggcccgg ccctgcttga ccacgtcctg gacggtgccc gccacgacgt
     3001 ccgccattcc ggtcaggacg aacgcggcct ctgccgcgac cttcgtggcg gcccggccga
     3061 ccttctcggg agtgatgcgg gtgtgcgacg agagtgggct tttcgacgac attgttcttc
     3121 tcctggtctc acgggcggga cgctcccccg cctgaacggc cagcctcgtc ggacgaagca
     3181 ggttcttcaa cgcttggccg gaggatccgg gtgcagtcag ggacatcgac gccgcgatgg
     3241 cgggcagcgt ccgtggtcgg tgcctggcag gtccgcgagc aggttcgcaa tcaggtgccg
     3301 cgggccgcac gcagctggct acgctgcggt catgaccctg cacgatgccg tgaaggcagt
     3361 ccttccccag gctctgctcg acctggccga gttggtcgcc attccctcgg tgagcgcgct
     3421 acccgcgcac gccggcgacg tccaacggtc ggccgaatgg atcgccgaca aactacgggc
     3481 gctccactgc cccgatgtac gcatcgttgc ggagggcgga gcgcccgcgg tgatcggccg
     3541 ctttcccgct ccgcccggtg cgccgacgat ctgcctctac gcacaccacg acgtgcagcc
     3601 caccggcgag ttggccgaat ggaccggtga cccgttcgtg gtgcatgagg ccgacgggcg
     3661 cctgttcgcc cgcggtgccg gcgacgacaa ggggggtgtg ctggtgcacc tcgccgcctt
     3721 gcgggcgttc gagggacgtc cgcccgtggg ggtcacggtg ttcgtcgagg gcgaggaaga
     3781 gatcggctca ccctcgctcg ccgacatcat tgcccgccac cacgacgagc tggccgccga
     3841 cgtgtacgtg atcgccgact cgggcaactg gcaggtgggc cggcccgcct tcaccgacac
     3901 gctgcgcggc ctggtcgact gcgtggtcga ggtgtcgacg ctcgaccacg cactgcactc
     3961 gggtcagttc ggcggcgcgg taccggatgc cctgaccacg ctgtgccggc tgctcgccac
     4021 cctgcacgac gatcagggaa acgtggccat cgaggggctg agcgcgatgc ccgatcccac
     4081 cctggactac ccgaaggagc ggctcctgac cgagaccggg ctgctggagg gcgtccacct
     4141 gatcggcgac gggtcgatcg ccggacggct gtggaccaag ccgaccgcca cggtgctggc
     4201 catcgacgcg aactcggtgg ccgaggcgtc caacattctc gcgcccacgg cacgggccaa
     4261 ggtgagcgtc cgcatcgcgc cggacgccga tccggccgcc gcgctgagcg cggtcaccga
     4321 gcacctgcgc cgcaacgcgc cgtggggcgc ccacgtcaac gtcatcgacg gctcaaccgg
     4381 ttcgggcacc acggtcgcgt tcaccgggcc ggccgccgaa gcggcgagag ccgcgttcgt
     4441 cgaggcctgg aacgtcgagc cggtgttcat cgggcagggc ggctcgattc cgatggtggc
     4501 ggactttcaa caggcctttc cggacgccac ggtgctggtc accgcggtct gcgaccccga
     4561 ttcacgcatg cacggcatcg acgagtcgct gaatctggac gacttcgcca aggcctgcct
     4621 ggccgaaacg ctgctgctgg ccaagctggc accgtagtcc gccggctttt cgcggttatc
     4681 gcggcgacct gcggttgggt cggtgcatta cgcgacttaa tggttgatac ggataccccc
     4741 gccgggtatt catagccacg agaaagcggc gggctgctcc cctgccgact cgacggcccg
     4801 gggaaccacc ataaagtggc gtgaccgatc ggaaccggct gatctacagc aacgacttga
     4861 cgatgccggt ggcggtgagt acggcctgcc gttcccagcc ctgcaggtcg gaggcccgga
     4921 ccgccttctc aaacgcggcc aggttgtcct tcgggttggt tccggccgcg atccggctgc
     4981 tggtggtgct ccaccgttgc cccaggtctg acttggccgc gctgtcgggc agtgagttca
     5041 gcgccaactg cacaccctgc agtgcggccg cgagcgcgac cgcatggccg ggatcgggcg
     5101 tggtggtctt cttcttggac gccgtcacgc tgggcttggg cgaggcgctg ctcggtcgtg
     5161 ccgaggcgga cgcgctgggg aacgcccgac ccaggttggt gagcgccagc ccgctcaggt
     5221 tgaccgccag gcccgcgacc agcacggccg ccatcaggct gagcagcacg cccgcgatcc
     5281 accacgagcc gggctgctgt ggcaggtgag ccgggccccg cacgccgtgc gaggcggccg
     5341 actgggcggg cgccggctgg gtgtagggcc gggtcggctg catgggggcc gcggtgggcg
     5401 gcggcgtggg cggcggcacg gcgaccggca ccgtggctgc cgacgggttg ccctgcagat
     5461 gttcgagcgc ccgcagcgtc gcggcggtgc tcggacgttc caacggggtc ttggccagca
     5521 gcccggccac cagcgcatcg aggccctgcg gaacgtccgg acgccgctgg ttcagtcgcg
     5581 gcggcgcgct gttgatctga tgtcccgcca ccaccaccgg ctggtcgccg tcgaagggcg
     5641 gacgtccggt catcatcgtc atgatcaggc agccgagcgc gtacaggtcg gtcgcggcgt
     5701 ccacccgaag cccggcggcc tgctcgggcg acatgtaggc ggccgtgccg atggtcatcg
     5761 acggcgcggt caggtgcggg tcggcctggc cggtcagttg cgcgatgccg aagtcgagca
     5821 gcttcacccg gccgccctcg gtgagcatca cgttggcggg cttgatgtcg cggtgcacca
     5881 gcccgaccgc atgcgcggcg gccaggccaa gggtcacctg gcgggcgacg tccagggtgg
     5941 cggtgagccc gagcgggccg cgtcgtgcga tcagttcggc cagcgactcg ccgcgcagca
     6001 gttccatcac caggtaggcg gtgctgccgt ccacgccgcc gtcgaaaacc gtgacggtat
     6061 aggggctggt cagccgggcg gtgagcacga tctcgcgctg aaaccgtccg gccagtgagg
     6121 ggtcgcgcac gccggaaagg tcaacggtct tgatcgcgac ctcacgatcg agccgaatgt
     6181 cgcgggcgcg ccagacccgt cccatgccgc cggcgccgat ctcgcgctcc aggcgatacc
     6241 gcgcggcgag tagctgctcg gtcatctact cgcaggtgcc catcgctcct gcaccgacgt
     6301 cggtgggggt cttgttgtcg tcgcctcggt accacaggcc cgagggggtg gtgaacaccc
     6361 acaccgacga ttcgccaagg gtgatcgcgc gcagtttgcc cttgggaaag gcggtcttga
     6421 gcttggcgaa ggtggtgtcg accgggtagc cgtcggccag ccgcagtgtg ctgcccagcg
     6481 gctggtcgag gttgaccacc cagctggtga aggtgcgcgg cgagttcttc gtgcccctgg
     6541 ccttgctctg aaacaggaac accccggcgc cgtacagcag ctgccggccg tagggagtgt
     6601 cgccgtccac ctcgcagacc ttgccggaat agctctcgtc ggccttgccg gccttggcgt
     6661 tgagcgccgc ggtgaccgtg gcctcggcgg tgccgaaggc gaactgatcg acacccttgg
     6721 gttgcagcac cagcaccgcg gacgcactgg gcgatgcact ggcggtcggg ctgaccgacg
     6781 ggctggacga gcgtgcggtg gcgctcggcg agtcgctcgc ggaggggctg ggcgagggct
     6841 ccgtcgaggg cccggacgac gtggcgggcg ccaccgggct cgacggcacg cccaccggtg
     6901 gcgtggttcc acaggcggtc agcagtgcca acacaccaac ggcgccgagc gcgcgcaacg
     6961 aaagagccat ggcacaacga taggtggcgc cgggcgcgac gggacggccg ccgcacctgg
     7021 acgggtcagt gcaggtcgct cttgcggatc agggcatgga cgcccgtggt gcggtcgacg
     7081 acttgactga ccgagaaatt cgccgcggcc gagaggtacg cgtacgccac ggcccgattc
     7141 agaccctggt cctgctgcag gaagtccagt gcgttgacca cggcccggcg catcgcgaca
     7201 ttgaggtcgt tgccctggcc gttaacactg ccgtccgggt cggagaggcc gatcgggatc
     7261 caactcgaag cggtctcggc gaacgggtac ctgaaggcga cgctcggtgc cgtgcccgag
     7321 cccttcttgc agacggtgag ccgaaaggtg ccacgcaacg aaccttccat cgcggtcagc
     7381 gccacctcac cgtcacccat ggcgtggtgc ggatcaccga cgtagaacag cgcaccctcg
     7441 gcggccaccg gcagatagaa cgtcgagccc accccgagat gcctgatgtc gatgttgcct
     7501 ccgccgagcg tgggcgggat cgagttggcg ctcggatcgg tgaggccgct cgcctgcgag
     7561 taggccacgc ccatcattcc gaagaacggg ttgaccggga accgcagccg ccgccgtccg
     7621 gaccgcatca ccgccttccc gccctcgagc gcggtgaaca ccgagacgtt gtggtagtcg
     7681 gtgggaatgc ctgaggagcg cccatcggtg tcgtgcggtg gcatcacctc atcgagcgtg
     7741 atgccggccg gcgccgtacc ggccgacgtg atcgccaacg cacccttgcc gtgccggctc
     7801 gacaccacgc cgtagggcac ccgcgcgcgc acgtccaggg tctcgatctt cagcacgtcg
     7861 cccggttcgg cgcccgcgac ccggatcggt ccggtgacca cgtgggggcc gtccacgtcg
     7921 aagctgcggg tggtgcgccg gtagttcgcc gcgatgtcga tcgcgtcgtc cagaatctcg
     7981 cgacggcgca cgccgtgctc gcccagccag gccaccggat cgcggccctg gtcttcaaga
     8041 atgccttcgt gcgagaccga atcgatggtc acggttcgtc cggaccgcat cgtcagcacg
     8101 ggcttggcat gcacggtggg cacgtatccc cacagcacct cgccggtgcg cgatgagagg
     8161 tagtggtcgc cgcgaatgcg gcccttgccc ggctggagaa cgccctcgta gaagccacgc
     8221 ggagcggcgg cggcttcagt agcggacgtc gccgcagcca gcgttccccc ggcaccgacg
     8281 gcggctgccg ccagcagcag gccgcggcga cccacccccg actcgaggat gcgcgcggcc
     8341 tgcgcgggat ccggctgcgt ggactggttg gtcatggtcg ctccttggac tcagtgccgt
     8401 cggcccgagt ggtcgatgcg attgaagacg ctagccagca cgcagacgcg cgccgtttcg
     8461 gctcggttgc ggcggtgtta cgcgagccgc ccgcagggtg caccgcggac gtgcgacggt
     8521 gcgatcgatg gcccgctcgg ccgacctgct tgagcgaagg tgcgcgcggg taggttgtgc
     8581 gcatgagccc caagaagagc gccacacctg ccgacgaacc cgcgagcggc aagcaggcgg
     8641 ccacgacgga agccaagccc gaagcgaagg gcgcgggcaa gagcccgaag gccggcaaga
     8701 acgcgaaacc gacggggaag agctccgcaa cgggcaaagg ctccacggca ggcaaggatt
     8761 ccacggcggg cagaggcccg aaggcgggca aaaccgcgaa accgacgggc aaggactcca
     8821 cgggcaagag ctccacgggc aagggctcga cgagcaggaa cgccaaacag ccgggccggg
     8881 gcgccaaggg ggcggcgact cgtccgtccg tcaaccagca gccggcgctg gagggcatgg
     8941 gtgccatcgt cacggccgag ggctgcggct ttcgggtctg ggcgccgcac gccgaatcgg
     9001 tcgcggtgac cggtacgttc aacgactggt cgacgacgtc cgacccgctg ggtggcgagg
     9061 gaaacgggtt ctggtacggc ttcgtgccgg gcgccaaggc cgccgatgag tacaagttcg
     9121 ccctcaccaa cggcgagcag agcctggagc ggatcgaccc gtacgcctac caggtgacca
     9181 actcggtggg caacggcatc gtctacgacc acgccgcctt cgactggcag aacgactcgt
     9241 tcagctgtcc ggcgcacaac gagttggtga tctacgaact gcacgtcggc tcgttcgccc
     9301 cgaagaccga gggcgaggtc ggcacgttcg atgaggtgca ggccagcatc gagcactttc
     9361 agcggctcgg catcagcgcg atccagttga tgccgtcggc tgagttcgcg ggcgactact
     9421 cctgggggta caacccggcc cacatcttcg cggtcgaaag cgcctacggc ggcccggacg
     9481 ccctgaagac cctggttcgc gaggcgcacc gggccggcat cgcggtgatc atggacgtgg
     9541 tctacaacca cttcggcccc accgatctcg acctgtggca attcgacggc tggagcgaga
     9601 acgacaaggg cggcatctac ttctacaacg actggcgctc gtcgaccccg tggggtgaca
     9661 cccgtcccga ctacggacgc ggcgaggtgc gcagctacat tcgcgacaac gccgagatgt
     9721 ggctgcgcga ctaccacgtg gacggcctgc gcttcgacat gaccccctat atgcgctcgg
     9781 tgaacgccgg caccctcgac ctgcccgacg gttggagcct gcagtcgtgg atcaaccgct
     9841 ggatccgtga acagtttccg gacgcgatca ccatcgccga agatctgcac ggcgagccgc
     9901 tggtgaccag cacggccccc gagggagccg cgtaccacgc gcaatgggag gccaagttcg
     9961 tccacccggt gcgcgaggcg atcatcgtgg ccagcgacgc ctaccggtcg g
//
LOCUS       DAOSUG010000034         9992 bp    DNA     linear   ENV 10-JUN-2024
DEFINITION  MAG TPA_asm: Micropruina sp. isolate AMR_MDS_2195
            NODE_3714_length_9992_cov_3.922411, whole genome shotgun sequence.
ACCESSION   DAOSUG010000034 DAOSUG010000000
VERSION     DAOSUG010000034.1
DBLINK      BioProject: PRJNA925477
            BioSample: SAMN32864262
            Sequence Read Archive: SRR4236673
KEYWORDS    WGS; ENV; Metagenome Assembled Genome; MAG; Third Party Data; TPA;
            TPA:assembly.
SOURCE      Micropruina sp. (wastewater metagenome)
  ORGANISM  Micropruina sp.
            Bacteria; Bacillati; Actinomycetota; Actinomycetes;
            Propionibacteriales; Nocardioidaceae; Micropruina.
REFERENCE   1  (bases 1 to 9992)
  AUTHORS   Abdulkadir,N., Saraiva,J.P., Zhang,J., Stolte,S., Gillor,O.,
            Harms,H. and Rocha,U.
  TITLE     Genome-centric analyses of 165 metagenomes show that mobile genetic
            elements are crucial for the transmission of antimicrobial
            resistance genes to pathogens in activated sludge and wastewater
  JOURNAL   Microbiol Spectr 12 (3), e0291823 (2024)
   PUBMED   38289113
REFERENCE   2  (bases 1 to 9992)
  AUTHORS   Abdulkadir,N.
  TITLE     Direct Submission
  JOURNAL   Submitted (24-MAR-2023) Environmental Microbiology, Helmholtz
            Center for Environmental Research -UFZ, Permoserstr 15, Leipzig
            04318, Germany
COMMENT     The annotation was added by the NCBI Prokaryotic Genome Annotation
            Pipeline (PGAP). Information about PGAP can be found here:
            https://www.ncbi.nlm.nih.gov/genome/annotation_prok/
            
            ##Genome-Assembly-Data-START##
            Assembly Method        :: SPAdes v. 3.11.1
            Genome Representation  :: Full
            Expected Final Version :: Yes
            Genome Coverage        :: Not Applicable
            Sequencing Technology  :: Illumina MiSeq
            ##Genome-Assembly-Data-END##
            
            ##Genome-Annotation-Data-START##
            Annotation Provider               :: NCBI
            Annotation Date                   :: 05/04/2023 04:57:10
            Annotation Pipeline               :: NCBI Prokaryotic Genome
                                                 Annotation Pipeline (PGAP)
            Annotation Method                 :: Best-placed reference protein
                                                 set; GeneMarkS-2+
            Annotation Software revision      :: 6.5
            Features Annotated                :: Gene; CDS; rRNA; tRNA; ncRNA
            Genes (total)                     :: 2,776
            CDSs (total)                      :: 2,750
            Genes (coding)                    :: 2,714
            CDSs (with protein)               :: 2,714
            Genes (RNA)                       :: 26
            tRNAs                             :: 24
            ncRNAs                            :: 2
            Pseudo Genes (total)              :: 36
            CDSs (without protein)            :: 36
            Pseudo Genes (ambiguous residues) :: 0 of 36
            Pseudo Genes (frameshifted)       :: 2 of 36
            Pseudo Genes (incomplete)         :: 32 of 36
            Pseudo Genes (internal stop)      :: 2 of 36
            ##Genome-Annotation-Data-END##
FEATURES             Location/Qualifiers
     source          1..9992
                     /organism="Micropruina sp."
                     /mol_type="genomic DNA"
                     /submitter_seqid="NODE_3714_length_9992_cov_3.922411"
                     /isolate="AMR_MDS_2195"
                     /isolation_source="Activated sludge of municipal
                     wastewater treatment plants"
                     /db_xref="taxon:2737536"
                     /environmental_sample
                     /geo_loc_name="Canada"
                     /lat_lon="49.25 N 123.1 W"
                     /collection_date="2015-06-16"
                     /metagenome_source="wastewater metagenome"
                     /note="metagenomic"
     gene            complement(<1..164)
                     /locus_tag="PLF56_02090"
     CDS             complement(<1..164)
                     /locus_tag="PLF56_02090"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_002513859.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_component: GO:0005840 - ribosome [Evidence IEA];
                     GO_function: GO:0003735 - structural constituent of
                     ribosome [Evidence IEA];
                     GO_process: GO:0006412 - translation [Evidence IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="50S ribosomal protein L27"
                     /protein_id="HOQ52383.1"
                     /translation="MAHKKGASSSRNGRDSNSQRLGVKRFGGQAVNAGEILVRQRGTH
                     FHPGEGVGRGG"
     gene            complement(176..484)
                     /gene="rplU"
                     /locus_tag="PLF56_02095"
     CDS             complement(176..484)
                     /gene="rplU"
                     /locus_tag="PLF56_02095"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_009639958.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_component: GO:0000311 - plastid large ribosomal subunit
                     [Evidence IEA];
                     GO_component: GO:0022625 - cytosolic large ribosomal
                     subunit [Evidence IEA];
                     GO_function: GO:0003735 - structural constituent of
                     ribosome [Evidence IEA];
                     GO_process: GO:0006412 - translation [Evidence IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="50S ribosomal protein L21"
                     /protein_id="HOQ52384.1"
                     /translation="MYAIVRSGGRQHKVAVGDVLEIDRVDAAVGDSVALTPLMLVDGE
                     SVTIEAKKLAKVSVSAEVLGEIKGKKIHILKYKNKTGYKKRQGHRQKYTQVKITGIEG
                     "
     gene            complement(647..832)
                     /locus_tag="PLF56_02100"
     CDS             complement(647..832)
                     /locus_tag="PLF56_02100"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_008364240.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="DUF1508 domain-containing protein"
                     /protein_id="HOQ52385.1"
                     /translation="MAGKFEVYEDASGKYRFRLKSGNGEVVATGQSYTSLAGAKAGVD
                     AVKRAAEGAAVVVLEKE"
     gene            989..1690
                     /locus_tag="PLF56_02105"
     CDS             989..1690
                     /locus_tag="PLF56_02105"
                     /EC_number="3.1.-.-"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_012921344.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_component: GO:0005829 - cytosol [Evidence IEA];
                     GO_function: GO:0016787 - hydrolase activity [Evidence
                     IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="dienelactone hydrolase family protein"
                     /protein_id="HOQ52386.1"
                     /translation="MNDLVEVPRPDGRLPVHRWLPAAGRGPGLVVIQEIFGVSEYVRL
                     RCAEFAAAGYVVYAPELYFRQGRLQFDENSPSYVQEGIGASQRLDWAQTTQDVSATLD
                     ALRTATETTGSVGIVGYCFGGGMGFQVASLNCPDALVSYYGSAIPGLLALAPQITCPS
                     QHHWGLADTFFPQDVVERVKQAVTATGDEVEVEFYTYPGAGHAFDNPHPMFFNEPASE
                     LARQRTLAFLKQHLH"
     gene            complement(1700..2755)
                     /locus_tag="PLF56_02110"
     CDS             complement(1700..2755)
                     /locus_tag="PLF56_02110"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_017935987.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="M48 family metallopeptidase"
                     /protein_id="HOQ52387.1"
                     /translation="MADTARIRLASISSRAWEHPADRGALVALRKLHGFDVILRQLSG
                     FLNERAVRMMLLGSAVRADHRQFARVYRLYREAGDALDAAELPELFIRADPTINAMTI
                     GLDKPVIVLNSGLLEVMDDDELRFVLGHELGHVLSGHAVYRTLLGAMLALSSTFLAIP
                     FGALGARGVIAALLEWQRKSELSGDRAGLLATQQPQVALHAQMKMASGGRIDELDADA
                     FLEQAGEYHANDDVRDVLLRVLMVETQTHPFAVVRAGELHRWVTSGDYTKVLAGEYPR
                     REDDAGASMSDEAAAAAASYTEEFKRFDASLGGLFGDLGQGLGQARDWLADRLRDLSG
                     PSPRENSRSERKPPAGW"
     gene            2896..3495
                     /locus_tag="PLF56_02115"
     CDS             2896..3495
                     /locus_tag="PLF56_02115"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_008355468.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_function: GO:0003677 - DNA binding [Evidence IEA];
                     GO_function: GO:0016987 - sigma factor activity [Evidence
                     IEA];
                     GO_process: GO:0006352 - DNA-templated transcription
                     initiation [Evidence IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="sigma-70 family RNA polymerase sigma factor"
                     /protein_id="HOQ52388.1"
                     /translation="MAASDVAGRSDATESSVWVRATACFTRWQGGDSGALDDLVRLMS
                     PVLWHTVRAYGLTQAAAEDAVQNTWLALVRNRHQVHEAAAIGGWLLTTARRTAWRTKS
                     TDAKTLAVEDTHLTAALPNSAAAEASALEQLAASALWQHVQTLDERCQRLLRIVAFDD
                     RPDYAGLAQSLGMPIGSIGPTRRRCLDKLRSSLEKGGHL"
     gene            3492..3968
                     /locus_tag="PLF56_02120"
     CDS             3492..3968
                     /locus_tag="PLF56_02120"
                     /inference="COORDINATES: ab initio
                     prediction:GeneMarkS-2+"
                     /note="Derived by automated computational analysis using
                     gene prediction method: GeneMarkS-2+."
                     /codon_start=1
                     /transl_table=11
                     /product="hypothetical protein"
                     /protein_id="HOQ52389.1"
                     /translation="MTEKRDPLLVGIKKMYSELDPVPEGLTERVLIALAVDDLELEYE
                     LLTMVHRSQQLAGVRGGDDQKLVMEFTAAGITVMLRISPVDAKHRRIDGWVTRTADAK
                     GELVELTASLWQEAGTATSPVGSHGRFEFASVPSGLTRLDLVADRDSAFRTTLFEI"
     gene            3996..>4962
                     /locus_tag="PLF56_02125"
     CDS             3996..>4962
                     /locus_tag="PLF56_02125"
                     /EC_number="3.4.-.-"
                     /inference="COORDINATES: ab initio
                     prediction:GeneMarkS-2+"
                     /note="Derived by automated computational analysis using
                     gene prediction method: GeneMarkS-2+.
                     GO_function: GO:0008236 - serine-type peptidase activity
                     [Evidence IEA];
                     GO_process: GO:0006508 - proteolysis [Evidence IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="S8/S53 family peptidase"
                     /protein_id="HOQ52390.1"
                     /translation="MSDYNSPDGQEKPDEPKAVVNKPVVRDPMDRDPDTPSREVLNQL
                     FGRVLDPGTAMQVKGLKTRPTAYIGHRILVPTDRLDDVLPLLQKAASFKGWTLEADPE
                     FPAWRVNVPDPDAPERRISLGQARFLIDIIPGQTSSSPDAWVLLQLARANSGIEALRG
                     VQLDHIVTPTPLYEPHPLYEPHPLYEPHPLYEPHGYPNRNPVAYIGEPPCRNEKLGSL
                     RPVVAIMDTGVGVHPWLTGVVNETVTLDGTPIGYTDPATDPEQNPDQEGPLDGGLDAS
                     SGHGTFIAGLIHQACPDADIISWRLIPSEGPLAESEVMATLAQVLE"
     assembly_gap    4963..4972
                     /estimated_length=10
                     /gap_type="within scaffold"
                     /linkage_evidence="paired-ends"
     gene            <4973..5586
                     /locus_tag="PLF56_02130"
     CDS             <4973..5586
                     /locus_tag="PLF56_02130"
                     /inference="COORDINATES: protein motif:HMM:NF012311.2"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_function: GO:0008236 - serine-type peptidase activity
                     [Evidence IEA];
                     GO_process: GO:0006508 - proteolysis [Evidence IEA]"
                     /codon_start=3
                     /transl_table=11
                     /product="S8 family serine peptidase"
                     /protein_id="HOQ52391.1"
                     /translation="FDGRTDGQRIDVLSLSYGFFHESAADTASELQLRTMLRALSVMG
                     TVVVCAAGNEATARPRYPAAFGPWRNDPSATLNAAELPIVSVGALNPNGTEALFSNTG
                     PWVRAWATGAALVSTIPSFKGGYLPSARIRYEGRIREGIDPDDFTGQFATWSGTSFSS
                     PLIAGRIAARLLKTMPGDEATHRATSVTRAWSCVAAETDIVAP"
     gene            5591..8008
                     /locus_tag="PLF56_02135"
     CDS             5591..8008
                     /locus_tag="PLF56_02135"
                     /inference="COORDINATES: protein motif:HMM:NF024180.2"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="CHAT domain-containing tetratricopeptide repeat
                     protein"
                     /protein_id="HOQ52392.1"
                     /translation="MLSADELHERAKAANSGGRFRQARRWLTTAIQRSTDPAQLARLE
                     STLSYVEVELGRPEVGLDLLDTALSRPGRLADGEIGVLLAQRALVRVRLGELEAAMPD
                     FAAAIRLLGHEPAELGKLFNNRGNLYLERGQPVRAIQDFENAIDQYDLSHNPVARAKA
                     EHNLGYAAMLQGDLATALQRMDHAHRLLRRESSALSVMASQDRAEAMLAAGLIDEALR
                     ALRSAAAGFGTMRLRRYQAEAEFVLARNLAPLDPTEAARTARRAARRFAGVGAPLQAL
                     RCRAIEVTCRIQRNVPVPDADELLADLREARLDRDALSIDVQLRARAGHSMTLPRDAP
                     LSVRLLAADSMAAKQARSGNAPAALSTIRRGLTELHRTQAALGSLDLQTALAVHGQRM
                     AELGLRIAVEKGSPRMVFEWVERTGALSGRVEPVRPPTDSAMASDLTELRMLSLRSPR
                     PGSHESERQAELLHLVRERAWRDPGSARLTDVVTMTALRAELSASDAVLVAPLSVDDQ
                     AWALLVTAISSRLVCLGPLREIASELRGLPADLDLEAAELPASIANSVRASLADRLRR
                     LDSLVFGLLGDQLTSERIVINPTGLFSGMPWTLMPSLTGRSVTIPRSASAWVTARAKP
                     HEYATAGFAAGPRLPRAGHEVASAAAYWPSAHVLAGSDATADSVGAMAGQVDLLHLAA
                     HGHHVADNPLFSNLELVDGPWFGYDLDQLPQVPETVVLSACELGATTIRRGDELLGLT
                     TAWLHAGARCVIASPASVSDEVAAAILPDMHAELAKGVPPADALATATAKHPDLLSTF
                     QCYGAGW"
     gene            complement(8120..8743)
                     /locus_tag="PLF56_02140"
     CDS             complement(8120..8743)
                     /locus_tag="PLF56_02140"
                     /inference="COORDINATES: ab initio
                     prediction:GeneMarkS-2+"
                     /note="Derived by automated computational analysis using
                     gene prediction method: GeneMarkS-2+."
                     /codon_start=1
                     /transl_table=11
                     /product="hydrolase"
                     /protein_id="HOQ52393.1"
                     /translation="MAPWLVDTPLGPCRLTVSAADDPRVVLLLGHGAGGGIEAFDLVA
                     LASGLPPHGVTVIRHEQPWRVAARKTVARPNVVDQGWRPALDVVVAAFPGLPLWVGGR
                     SAGARGACRNVDARQVGVVCLSFPLHPPGRPEVSRIAELAGVGGPVLVIQGESDPFGS
                     PQDVRDAARVAGGAQPLVIGQPGTHSFSRRADVRDSIVAATAKFVTG"
     gene            8796..>9992
                     /locus_tag="PLF56_02145"
     CDS             8796..>9992
                     /locus_tag="PLF56_02145"
                     /inference="COORDINATES: protein motif:HMM:NF021489.2"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="DUF2207 domain-containing protein"
                     /protein_id="HOQ52394.1"
                     /translation="MDLTWILAGIAVVAVLAWAWRAQRAKFADEYFDGVTPGLTPVGG
                     QPDHRVPVPGGSEYSGEIAVAFNPPRGVRPGLGGTVVDGNPESRDVMATLVDLAARGH
                     LSITIVEDESRRNGKDWELHVADVPPRDELDPSEQQLLHDVFVTSPDVRLSDLPRQGN
                     HAFNDHQFALANQSYERGFFRLVTGMHPVRLVWIAAGLLLMLGFATGSGVALASAAVL
                     ALGGALIGARSTQRPVRTAEGTALRIQTLGFKRYLETAEVEQFSYEEAAGIFSKYLPW
                     AIVLGVAGHWVKIFGDLAAAARADGYEDDMDLLWLGVVGWGVNDAMFSLAMFSAMDGF
                     GDMGGMGDAFGDPGSMFDSGAIDGLGADAGGAFGDFGGGDFGGGMDGGAGADVGGGWG
                     DFGGGGD"
BASE COUNT         1619 a         3498 c         3239 g         1626 t
ORIGIN      
        1 ccaccgcgac ccacgccctc accggggtgg aaatgggtgc cgcgctggcg aaccaggatc
       61 tcgcccgcgt tgacggcctg accgccgaag cgcttcacgc cgagccgctg cgagttggag
      121 tcgcgaccgt tgcgtgagct ggatgcgccc ttcttatgtg ccatctcgta ctacctcagc
      181 cttcgattcc ggtgatcttg acctgggtgt acttctgccg gtggccctgg cgcttcttgt
      241 acccggtctt gttcttgtac ttcaggatgt ggatcttctt acccttgatc tcacccagca
      301 cctcggcgct gacgctgacc ttggccagct tcttggcttc gatggtgacg gactcgccgt
      361 cgacgagcat cagcggagtc agcgcaacgc tgtcacccac cgccgcatcg acccggtcga
      421 tctccaggac gtcgccgact gcaaccttgt gctggcggcc gccactgcgc acgatcgcgt
      481 acacgcctga cccactctct tcgaactttc acgaatccgt gaacttcacg attccggtgc
      541 cgggacgggc gactggaaca agtccaagtc agccgacgag aaacccacgg gcaccgagga
      601 tcaagaatac gtgcccagcc cgaacagggt caaaccggcg tgccggttac tccttttcga
      661 gcacgaccac cgccgcgcct tcggccgccc gcttgacggc gtccacgccg gccttggcac
      721 cggcgaggct ggtgtaggac tgcccggtcg cgacgacctc accgttgccg gacttcaacc
      781 gaaaacggta cttgccggac gcgtcctcgt agacctcgaa cttgcctgcc atgacggtct
      841 cctcactggt cacgggacgc ctgtcgtccg cattcatgct aatcgggctc tacctcaaag
      901 ggcgtgccat cggtgcgagt tggccagcgt gtccggacgg agtgaccgcg tcgtttgtgc
      961 cggccacgac gtgcggcagg atgggcccgt gaacgatctc gtcgaggtac cgcgtccgga
     1021 cgggcgactg ccggtccacc gctggctccc tgctgctgga cgcgggccgg gcctggtggt
     1081 gattcaagag atcttcggcg tcagcgagta cgtccggctg cgttgcgccg agttcgccgc
     1141 cgcgggctac gtcgtctacg cacccgagtt gtactttcgc cagggacggc tccagttcga
     1201 tgagaacagc ccgagctatg tgcaggaggg catcggcgcc tcccagcggc tcgactgggc
     1261 ccagaccacg caggacgtct ccgccacgct ggacgccctg cggaccgcga cggagaccac
     1321 cggcagcgtc gggatcgtcg gctactgctt cggcggcggc atgggttttc aggtcgcttc
     1381 gctgaactgc cccgatgccc tggtcagcta ctacggctcg gccatccccg gcctgctggc
     1441 gctggccccg cagatcacct gccccagcca gcaccactgg gggctcgccg acaccttctt
     1501 tccccaggac gtggtcgaac gggtcaagca ggcggtcacg gcgaccgggg acgaggtcga
     1561 ggtcgagttc tacacctacc cgggcgccgg ccacgccttc gacaacccgc acccgatgtt
     1621 cttcaacgag ccggcctccg agttggcccg tcagcgcacg ctcgccttcc tgaagcagca
     1681 cctgcactga gccgacccgt caccagccgg ccgggggctt gcgctccgag cgcgagttct
     1741 cgcgcggtga cggccccgac aggtcgcgca agcggtccgc cagccagtcc ctggcctgcc
     1801 ccagcccctg gcccagatcg ccgaagaggc cgcccaggct ggcatcgaaa cgcttgaact
     1861 cctcggtgta ggacgccgcc gctgccgcgg cctcgtcgga catgctggcg ccggcatcgt
     1921 cctcacggcg cggatactcg cccgccagca ccttcgtgta gtcacccgag gtcacccacc
     1981 ggtgcaactc gccggcccgg accaccgcga acgggtgcgt ctgggtctcg accatcagca
     2041 cccgcaacag cacgtcacgg acgtcgtcgt tggcgtgata ctcccccgcc tgttcgagaa
     2101 aggcgtcggc gtccaactcg tcgatccggc cgcccgaggc catcttcatc tgcgcgtgca
     2161 gcgccacctg cggctgctgc gtggccaaca gcccggcccg atcgcccgag agctcagact
     2221 tgcgctgcca ttccagcagc gccgcgatca ccccgcgtgc accgagcgca ccgaacggga
     2281 tcgccaaaaa ggtggacgac agcgccagca tcgcccccaa cagcgtgcgg tacacggcgt
     2341 ggccactgag cacatgcccc agctcgtggc cgagcacgaa ccgcagctcg tcgtcgtcca
     2401 tcacctcgag cagcccggag ttgagcacga tcaccggctt gtccagcccg atcgtcatgg
     2461 cgttgatggt gggatcggca cgtatgaaca gctcgggcag ctcagcggcg tccagggcat
     2521 caccggcctc gcggtagagc cggtacaccc gcgcgaactg ccggtggtcg gccctgaccg
     2581 ccgagcccag cagcatcatg cgcaccgccc gttcgttcaa gaagcccgac agttgccgaa
     2641 ggatcacgtc gaaaccgtgc agcttgcgca gggccaccag ggcaccccgg tcggccggat
     2701 gctcccaggc gcgcgagctg atcgaagcca gtcgaatacg tgctgtgtca gccatccacc
     2761 cagtgtgccc tgccgccgcg ctgcccgaag cggtaggttt ctgccacacc ttcgtggtga
     2821 gcagcatcac caggctgcgc acgtatcagg agccccggga gcgactcttg agggtttgtg
     2881 gaaacaggga cgctgatggc tgcatcggat gtggcgggac gctcggacgc gacggaatcc
     2941 tccgtctggg ttcgggccac tgcgtgtttc acgcgctggc agggcggcga ttccggcgct
     3001 ctggacgacc tggtgcgact gatgtcgccc gtgctgtggc acacggtgcg cgcgtacggg
     3061 ttgacccagg cggcggccga ggacgccgtg cagaacacct ggctggcgct ggtccgcaac
     3121 cgacatcagg tgcacgaggc cgccgcgatc ggtggctggc tgctcacgac ggcgcgacgc
     3181 accgcctggc gcaccaagtc gaccgatgcc aagacgctgg ccgtcgaaga cacgcacctg
     3241 accgccgccc tgcccaactc ggcggccgcc gaggccagcg cgctggaaca gctagccgca
     3301 tcagcgctgt ggcagcacgt ccagacgttg gacgagcggt gccagcggct gttgcgaatc
     3361 gtcgccttcg acgaccgtcc tgactacgcg ggtctcgccc aatccctggg catgcccatc
     3421 ggcagcatcg gccccacccg gcgccgctgt ctcgacaaac tgcgcagctc gctggagaaa
     3481 ggtggccacc tgtgaccgag aaacgtgacc cgctgctggt cggcatcaag aagatgtaca
     3541 gcgaactgga cccggtaccc gaggggttga ccgaacgggt cctgatcgcc ctcgccgtgg
     3601 acgacctcga gctcgagtac gagctgctca ccatggtgca ccgctcccag caactggccg
     3661 gggtccgcgg cggcgacgac cagaagctgg tgatggagtt caccgccgcc ggcatcacgg
     3721 tgatgctgcg gatcagcccg gtcgacgcga agcatcgccg gatcgacggc tgggtcaccc
     3781 gcaccgccga cgccaagggc gagctggtcg aactcaccgc ttcgttgtgg caggaggccg
     3841 gcaccgccac ctcgccggtc ggcagccacg gccggttcga gttcgccagt gtgcccagcg
     3901 gactgacccg gctcgacctg gtcgccgacc gtgattccgc cttccgcacc accctgttcg
     3961 agatctgagt tgccccctac cccgaggacg aactgatgag cgactacaac agccctgacg
     4021 gccaggaaaa gcctgacgaa ccgaaggctg tggtgaacaa accggtcgtc cgcgacccca
     4081 tggatcgcga tccggataca ccttcgcgcg aggtactcaa ccaactgttc ggacgagtgc
     4141 tggacccggg taccgcgatg caggtgaagg gcctgaagac gcgcccgacc gcctatatcg
     4201 ggcaccgcat cctggtgccg accgatcgac tcgacgacgt cctcccgctg cttcagaaag
     4261 ctgcctcgtt caaaggatgg acacttgagg ccgatccgga gttcccggcg tggcgggtga
     4321 acgtccccga cccggacgca ccagaacgcc gaatcagcct gggacaggca cgtttcctga
     4381 tcgacatcat tcccggccag acctcctcgt cgccggacgc ttgggtgctg ctgcagcttg
     4441 cccgtgccaa cagtgggatc gaggctctgc gcggagtaca actcgatcac atcgtcacgc
     4501 ccacacccct gtacgaaccc caccctctgt acgagccaca tcccctgtac gagccgcatc
     4561 ccctctacga accgcacggg tatcccaacc gcaacccggt ggcctacatc ggcgagccgc
     4621 catgccgcaa cgagaagctc ggctcccttc gcccggtggt ggcgatcatg gacaccggag
     4681 tcggcgtcca cccctggcta acgggtgtgg tgaacgagac ggtcactttg gacggcacgc
     4741 ccatcggcta caccgatccc gccaccgacc cagaacagaa tccggaccag gaaggcccgc
     4801 tcgacggagg cctggacgcg tcatccggac atggcacctt catcgccggc ctgattcatc
     4861 aggcatgccc tgatgccgac atcatctcgt ggcgcctcat tccttccgaa ggccccctcg
     4921 ccgagtccga ggtgatggca acattggcgc aagtgctcga acnnnnnnnn nnacttcgac
     4981 ggacgcaccg acgggcaacg cattgacgtg ctcagcctgt cgtacggctt cttccacgag
     5041 agtgcagcgg atacggcttc ggagttgcag ttgcgcacca tgctgcgggc cttgtcggta
     5101 atgggcacgg tcgtggtatg tgctgctggt aacgaggcca ccgcccgtcc ccgctacccg
     5161 gctgccttcg gcccgtggcg caatgacccc tccgccactc tcaatgccgc tgagctaccc
     5221 atcgtgtcgg tcggcgctct gaatcccaac ggaacggaag cgctgttcag caacacgggt
     5281 ccgtgggtcc gcgcgtgggc caccggcgcc gctctggtca gcaccatccc gagtttcaag
     5341 ggtggttatc taccgtccgc gcgcattcgc tacgaaggtc gcatccgtga gggcatcgac
     5401 cccgacgatt tcaccgggca gttcgccacg tggagcggaa cgtcgttctc atccccgctg
     5461 atcgctggac ggatcgcggc gcggctgctc aagaccatgc cgggtgacga agcaacccat
     5521 cgcgcgacat cggttacccg ggcatggtcc tgcgtcgccg ctgagacaga catcgtggca
     5581 ccataacggg gtgctgagcg ccgatgagct gcatgagcgt gcgaaggccg caaacagcgg
     5641 cggacggttc aggcaggcgc gacgctggct cacgacggcc atccagcgtt cgaccgatcc
     5701 ggcgcagttg gcgcgcttgg agagcacgct cagttacgtc gaggtggagt tgggtcgccc
     5761 agaggtcggt cttgacctgc tcgacacagc gctgagtcgt cccggacggt tggccgatgg
     5821 cgagataggc gtgctgcttg cccagcgtgc attggtcagg gtgcgtctgg gcgagctgga
     5881 ggcggctatg ccagacttcg ccgcggcgat tcggctgctc ggtcacgagc cggcagagct
     5941 cggcaagttg ttcaacaatc gcggcaatct ctacctcgaa cgcggtcaac cggtccgtgc
     6001 aatacaggac ttcgagaatg caatagacca gtacgacctc agccacaacc ccgtggcgcg
     6061 tgcaaaagcc gaacacaacc tgggttacgc cgcgatgctg cagggcgatc tggcgaccgc
     6121 gctccagcgg atggatcacg cgcaccgcct gttgcgtcgc gagtcgtccg cactgagcgt
     6181 catggctagc caggacaggg ccgaggcgat gctggccgcg ggcctgatcg acgaggccct
     6241 gcgtgcactt cgcagcgccg ccgcgggctt cggcacgatg cgactgcgcc ggtatcaggc
     6301 cgaggccgag ttcgtgctgg cccggaacct ggctccgctc gaccctacgg aggcagcaag
     6361 gaccgcccga cgagcagccc gacgcttcgc gggcgtcggt gcaccacttc aggcactgcg
     6421 ctgtcgggcg atcgaagtca cgtgcaggat tcagcgcaat gtgccggtgc ccgatgccga
     6481 tgagttgctg gccgatctgc gcgaagcccg gcttgatcgt gacgcactgt cgatcgatgt
     6541 tcagctccgt gctcgcgccg gccattcaat gacacttccg cgcgacgcac ccctgtcggt
     6601 gcgactgttg gcggccgact cgatggcagc caaacaggca cgttcgggca acgcccctgc
     6661 cgctttgagc accattcgtc gcggcctcac cgaactgcac cgcacccaag ccgctctggg
     6721 cagcctcgac ctgcaaacgg cactggccgt gcatggccaa cggatggccg aactcggcct
     6781 gcgaatcgct gtggagaagg gttctccgcg gatggtgttc gaatgggttg agcgaaccgg
     6841 cgccttgtcg ggccgggtgg agccggtccg tccaccgacc gactcggcga tggcgtccga
     6901 cttgaccgag cttcgcatgc tcagcctgcg aagcccccgt cccggcagtc atgagtcaga
     6961 acggcaggcc gaactcctgc atcttgttcg ggagcgtgcc tggcgcgacc ccggatcggc
     7021 cagattgacc gacgtggtca cgatgaccgc tctcagagcc gaactgtcgg cgtcagatgc
     7081 cgtcctggtg gcgccacttt ccgtcgatga ccaggcttgg gcgttgctgg tcaccgccat
     7141 ctccagccgc ctggtttgcc tgggcccgtt gcgcgagatc gcctccgagt tgagaggcct
     7201 gcccgctgac ctggatctcg aggctgcaga gctaccggct tccatcgcca attcggtgcg
     7261 agcctcgctg gccgaccggc tccgccgcct cgactcgctg gtctttgggt tgttgggcga
     7321 ccaactcacc agcgaacgaa tcgtgatcaa tccgactggg ctcttctccg gcatgccctg
     7381 gacgctgatg ccgtctctga ccggtcgctc tgtcaccatt ccgcgcagcg catcggcgtg
     7441 ggtgacggcc cgggcgaagc cacacgagta cgcaaccgca ggcttcgcgg ccgggccccg
     7501 gcttccccgg gccggtcatg aggtggcctc ggccgctgcg tactggccga gcgcccacgt
     7561 gctggccggt tcggacgcca ccgcagactc ggtcggcgcg atggccggcc aggtcgacct
     7621 actgcacctc gcagcgcacg gccaccacgt ggccgacaac ccgctgttca gcaacctcga
     7681 actggtggac ggtccctggt tcggctacga cctcgatcaa ctgccgcagg tgcccgagac
     7741 ggtggtcctg tcggcctgtg agttgggtgc caccacgatt cggcgcggcg acgaactgct
     7801 cgggctcact accgcgtggc tgcatgcggg cgcccgctgc gtgatcgcgt ccccggcctc
     7861 ggtctcagac gaagtggcgg cggcgatcct gcccgacatg cacgccgaac tggccaaggg
     7921 cgtgccgccc gccgacgcgt tggcgacggc aaccgccaag caccccgacc tgctttccac
     7981 cttccagtgc tacggcgccg gctggtagct gagcagggcc ggacccgaac gcgcgaaatg
     8041 tgcgcgccag tgagggccgg gcggcccgac acgccgtccg ccgacgcact ggcgcgcact
     8101 tttcgcacat tgcggccggc tacccggtga cgaacttcgc cgtggccgcc acgatcgagt
     8161 cccgcacatc ggctcgacga ctgaacgaat gggtgcccgg ttggccgatc accagcggct
     8221 gcgctccccc ggccactctc gccgcgtccc ggacgtcctg gggcgagccg aacgggtcgc
     8281 tctcgccctg gatgaccagc accgggccgc cgaccccggc caactccgcg atccttgaca
     8341 cctccggccg ccccggcggg tgcagcggga atgacaggca caccaccccg acctgccgtg
     8401 cgtcgacgtt gcgacaggca ccccgtgcgc cggccgagcg tccacccacc cagagcggca
     8461 ggccggggaa ggcggccacg accacgtcca acgccggtcg ccaaccctga tcgaccacgt
     8521 tcggacgggc cacggtcttt cttgcggcca ctcgccacgg ttgctcgtgc cggatcaccg
     8581 tcaccccatg cggcgggagg ccgctggcca gcgccaccag gtcgaaggcc tcgatgccac
     8641 ccccggcgcc atgtccgagc agcagcacga cgcgcggatc gtccgctgcc gagactgtga
     8701 ggcgacaggg tccgagcgga gtgtcgacga gccagggagc catgcgccga ttgtgtcgcc
     8761 aggacggcta ccgcgcacct gtcaccatgg agaacatgga cctcacctgg attctcgctg
     8821 ggatcgccgt ggttgccgtg ctggcgtggg cgtggcgcgc gcaacgtgcc aagtttgccg
     8881 acgagtactt cgacggcgtc acccctgggc tcacccccgt cggcggccag ccggaccacc
     8941 gggtgccggt gccgggcggg tccgagtact cgggcgagat cgcggtggcg ttcaatccgc
     9001 cgcgcggggt gcgtccgggg ctgggtggca ccgtggtcga cggcaacccc gaaagccgcg
     9061 acgtgatggc cactctcgtc gaccttgccg ctcgcggcca cctgagtatc acgatcgtcg
     9121 aggacgagtc gcggcgcaac ggtaaggact gggaactgca cgtggcggat gtgccacccc
     9181 gcgacgaact cgacccgtcc gagcagcagc tgttgcacga cgtcttcgtc accagcccgg
     9241 acgtgcgcct ctcggacctg ccccgccagg gcaaccacgc gttcaacgat caccagttcg
     9301 cactggccaa ccagagctat gagcgcggct tcttccggct ggtcaccggc atgcacccgg
     9361 tacggctggt gtggatcgcc gccggattgc tgttgatgct cggcttcgcg accggcagcg
     9421 gcgtggcgtt ggcgtccgcg gcggtactgg cactgggcgg tgcgctgatt ggggcgcgca
     9481 gcacgcagcg tccggtgcgc acggccgaag gcaccgccct gcgcattcag acccttggtt
     9541 tcaagcgcta cctcgaaacc gccgaggtcg agcagttcag ctatgaggag gccgccggca
     9601 tcttctccaa gtacctgccc tgggcgatcg tgctgggcgt ggccgggcat tgggtgaaga
     9661 tcttcggcga cctggcggcc gccgcccgag ccgacggcta cgaagacgac atggacctgc
     9721 tctggctcgg cgtggtcggc tggggtgtga acgacgccat gttcagcctg gccatgttct
     9781 ctgccatgga cggcttcggc gacatgggcg gcatgggcga cgcgttcggc gacccggggt
     9841 cgatgttcga cagcggagcg atcgacggac tgggcgccga cgcgggtggt gcgttcggcg
     9901 acttcggcgg cggcgacttc ggcggcggca tggacggtgg tgccggcgcc gacgtgggcg
     9961 gcggttgggg cgacttcggc ggcggtggcg ac
//
LOCUS       DAOSUG010000035         9911 bp    DNA     linear   ENV 10-JUN-2024
DEFINITION  MAG TPA_asm: Micropruina sp. isolate AMR_MDS_2195
            NODE_3747_length_9911_cov_3.698458, whole genome shotgun sequence.
ACCESSION   DAOSUG010000035 DAOSUG010000000
VERSION     DAOSUG010000035.1
DBLINK      BioProject: PRJNA925477
            BioSample: SAMN32864262
            Sequence Read Archive: SRR4236673
KEYWORDS    WGS; ENV; Metagenome Assembled Genome; MAG; Third Party Data; TPA;
            TPA:assembly.
SOURCE      Micropruina sp. (wastewater metagenome)
  ORGANISM  Micropruina sp.
            Bacteria; Bacillati; Actinomycetota; Actinomycetes;
            Propionibacteriales; Nocardioidaceae; Micropruina.
REFERENCE   1  (bases 1 to 9911)
  AUTHORS   Abdulkadir,N., Saraiva,J.P., Zhang,J., Stolte,S., Gillor,O.,
            Harms,H. and Rocha,U.
  TITLE     Genome-centric analyses of 165 metagenomes show that mobile genetic
            elements are crucial for the transmission of antimicrobial
            resistance genes to pathogens in activated sludge and wastewater
  JOURNAL   Microbiol Spectr 12 (3), e0291823 (2024)
   PUBMED   38289113
REFERENCE   2  (bases 1 to 9911)
  AUTHORS   Abdulkadir,N.
  TITLE     Direct Submission
  JOURNAL   Submitted (24-MAR-2023) Environmental Microbiology, Helmholtz
            Center for Environmental Research -UFZ, Permoserstr 15, Leipzig
            04318, Germany
COMMENT     The annotation was added by the NCBI Prokaryotic Genome Annotation
            Pipeline (PGAP). Information about PGAP can be found here:
            https://www.ncbi.nlm.nih.gov/genome/annotation_prok/
            
            ##Genome-Assembly-Data-START##
            Assembly Method        :: SPAdes v. 3.11.1
            Genome Representation  :: Full
            Expected Final Version :: Yes
            Genome Coverage        :: Not Applicable
            Sequencing Technology  :: Illumina MiSeq
            ##Genome-Assembly-Data-END##
            
            ##Genome-Annotation-Data-START##
            Annotation Provider               :: NCBI
            Annotation Date                   :: 05/04/2023 04:57:10
            Annotation Pipeline               :: NCBI Prokaryotic Genome
                                                 Annotation Pipeline (PGAP)
            Annotation Method                 :: Best-placed reference protein
                                                 set; GeneMarkS-2+
            Annotation Software revision      :: 6.5
            Features Annotated                :: Gene; CDS; rRNA; tRNA; ncRNA
            Genes (total)                     :: 2,776
            CDSs (total)                      :: 2,750
            Genes (coding)                    :: 2,714
            CDSs (with protein)               :: 2,714
            Genes (RNA)                       :: 26
            tRNAs                             :: 24
            ncRNAs                            :: 2
            Pseudo Genes (total)              :: 36
            CDSs (without protein)            :: 36
            Pseudo Genes (ambiguous residues) :: 0 of 36
            Pseudo Genes (frameshifted)       :: 2 of 36
            Pseudo Genes (incomplete)         :: 32 of 36
            Pseudo Genes (internal stop)      :: 2 of 36
            ##Genome-Annotation-Data-END##
FEATURES             Location/Qualifiers
     source          1..9911
                     /organism="Micropruina sp."
                     /mol_type="genomic DNA"
                     /submitter_seqid="NODE_3747_length_9911_cov_3.698458"
                     /isolate="AMR_MDS_2195"
                     /isolation_source="Activated sludge of municipal
                     wastewater treatment plants"
                     /db_xref="taxon:2737536"
                     /environmental_sample
                     /geo_loc_name="Canada"
                     /lat_lon="49.25 N 123.1 W"
                     /collection_date="2015-06-16"
                     /metagenome_source="wastewater metagenome"
                     /note="metagenomic"
     gene            complement(<1..575)
                     /locus_tag="PLF56_02150"
     CDS             complement(<1..575)
                     /locus_tag="PLF56_02150"
                     /inference="COORDINATES: ab initio
                     prediction:GeneMarkS-2+"
                     /note="Derived by automated computational analysis using
                     gene prediction method: GeneMarkS-2+."
                     /codon_start=1
                     /transl_table=11
                     /product="hypothetical protein"
                     /protein_id="HOQ52395.1"
                     /translation="MDLLGAVLIVAAVAATFVVITRNKAWKGRRARAEKDSQLAMAKR
                     TAAADVVGLVEALNRLVAVTENDPVAKAEFQHAEAARLRAVRCLHEATEPDELCLVTE
                     NLEEGRWTVARLAARAAGEPMPSRRPPCFFNPGHGPSARNIHWHGLSVPACSADAARV
                     DAGADPYIRTVERDSHRVPYWEGGPAYAGWAR"
     gene            complement(683..2458)
                     /locus_tag="PLF56_02155"
     CDS             complement(683..2458)
                     /locus_tag="PLF56_02155"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_007078463.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="biotin carboxylase N-terminal domain-containing
                     protein"
                     /protein_id="HOQ52396.1"
                     /translation="MASGELFSKVLVANRGEIAVRVIRAARDAGIGSVACYADQDADS
                     LFVALADEAFALGGTTPAETYLDIAKILAVAEKSGAQAVHPGYGFLAENAAFAQAVID
                     AGLTWVGPPPAAIESLGDKVQARHIAARVGAPLVPGTKDPVAGADEVVAFAKEHGLPI
                     AIKAAYGGGGRGLKVARNLDEVAELFESAVREAVTAFGRGECFVERYLDRPRHVETQC
                     LADQHGNVVVVSTRDCSLQRRHQKLVEEAPAPFLTDDQIERLYSSSKAILREAGYVGA
                     GTCEFLVGQDGTISFLEVNTRLQVEHPVSEEVTGIDLVREMFRIAAGEALGYDDPVPR
                     AHSIEFRLNAEDAGRNFMPAPGTLAVWQPPAGPGVRVDEGYLAGMTVPSAFDSLVAKI
                     IVTGADRSQALQRSRRALAETRIEGMPSVLPFQLAVLNDPAYTAADGAFGVHTRWIET
                     EFTGTIAPYQGASEAVEASERQSFVVEVDGRRLEVSLPGELLAPVKRSAQPTRTPRRS
                     GGAGKSAAVASADLTAPMQGTIVRVAVAEGDEVAEGDLVVVLEAMKMEQPISAHRAGT
                     ITNLKAVAGATVTSGAVLCTIDDAN"
     gene            complement(2504..2716)
                     /locus_tag="PLF56_02160"
     CDS             complement(2504..2716)
                     /locus_tag="PLF56_02160"
                     /inference="COORDINATES: protein motif:HMM:NF025192.2"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="acyl-CoA carboxylase epsilon subunit"
                     /protein_id="HOQ52397.1"
                     /translation="MTAPIIVRGRPTDLELAALAAALVALRTAAAEAGADEPPARGAQ
                     GWRSRHLQLRTRPGPGPGAWRASVRH"
     gene            complement(2713..4284)
                     /locus_tag="PLF56_02165"
     CDS             complement(2713..4284)
                     /locus_tag="PLF56_02165"
                     /EC_number="6.-.-.-"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_002514707.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_function: GO:0016874 - ligase activity [Evidence IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="acyl-CoA carboxylase subunit beta"
                     /protein_id="HOQ52398.1"
                     /translation="MEFDIHTTSGKLADFQRRNEKAKHPASDAAVAKIHDRGKLTARE
                     RVLALLDPDSFTELDEFARHRTTAFGMDAKRPYTDGVITGMGTIDGRNVCVFSQDVTI
                     FGGALGEVYGEKICKIMDLAMRTGVPIIGLVEGGGARIQEGVVSLGLYAEIFKRNTHA
                     SGVIPQISVIMGAAAGGHVYSPALTDVVVMVDQTSQMFITGPDVIRTVTGEEVSMEEL
                     GGARTHNTKSGNAHYLAADEQDALEFVRHVLSFLPANNLDEPPTLEVDLDLEHAADAE
                     ALDALIPDSPNQPYDMHEVITRVLDEGEFCEIHARFAANIVCGFGRVEGHPVGVVANQ
                     PMVYAGCLDIDASEKAARFVRFCDAFNVPVLTLVDVPGFLPGTDQEWSGIIRRGAKLL
                     YAYAEATVPLVTLITRKAYGGAYDVMGSKHLGADVNLAWPTAQIAVMGAEGAVNILYR
                     RELAESSDPVTRRAELIEAYNDELSNPWIAAERGYVDRVILPRDTRAEIAKVLRLLRT
                     KRASLPPKKHGNIPL"
     gene            4587..5789
                     /locus_tag="PLF56_02170"
     CDS             4587..5789
                     /locus_tag="PLF56_02170"
                     /EC_number="2.6.1.1"
                     /inference="COORDINATES: protein motif:HMM:NF004769.0"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="aspartate transaminase"
                     /protein_id="HOQ52399.1"
                     /translation="MTAEAARMSRVQPSPSTVAAGRARELREQGVAVLDLTVGEPDFD
                     TPDNVKAAGIAAIERGETKYTAVNGTAPLRRAIIDRLRRLTGLTYTDAEISVGSGAKH
                     VIFTALMATLSPGDEVVIPAPYWVSYPDMVVLNDGVPVVVPCPIEDDFKLTPDRLREA
                     LTGRTRWLILNAPSNPTGAVYRPDELRALAEVLRAFPQVLVLTDEIYDEVFLGAGRCT
                     GLLEVAPDLRERVLIVNGVSKTYAMTGWRLGYCAGPSAVVGAINKLQSHMASCPSSIS
                     QAAAVEALSGDQSFVADAAAQYRRRRDLVVERVNAVDGLRALAGDGAFYVFVDCSGVI
                     GRHTPSGVVIDSDDAFVGYLLDQARVATITGSAYGLSPFFRVSFATSEAVLIAAMDAI
                     AAAVAQLQ"
     gene            5821..6495
                     /locus_tag="PLF56_02175"
     CDS             5821..6495
                     /locus_tag="PLF56_02175"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_013862867.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_function: GO:0047443 -
                     4-hydroxy-4-methyl-2-oxoglutarate aldolase activity
                     [Evidence IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="4-carboxy-4-hydroxy-2-oxoadipate
                     aldolase/oxaloacetate decarboxylase"
                     /protein_id="HOQ52400.1"
                     /translation="MIHVRTRIERPDPALVAGLAQYSSATIHEAQGRRGALDSSLKPI
                     DRSMSFCGPAVTVVCAPRDNLMLQVAIHYAQRGDVLVVSAGEFGEAGTFGDVLGTAMH
                     SKGLAALVTDSGVRDTADLIDLGLPVFSRSVCIKGTVKETLGPINHPIVFGGELVEPG
                     DIVKGDADGVVLVKAADAGWVIEACAEREASEAELRELYRAGGTTIELCNLVEKLAAK
                     GLLVEE"
     gene            6985..7890
                     /locus_tag="PLF56_02180"
     CDS             6985..7890
                     /locus_tag="PLF56_02180"
                     /inference="COORDINATES: protein motif:HMM:NF016370.2"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="DUF559 domain-containing protein"
                     /protein_id="HOQ52401.1"
                     /translation="MDSDDLVMLMHVMSLNNGVLRPRDHPNSRATLARLVRSGAIVKV
                     LPGTYVDADRCTERRTRYAAALAFAPGSVLWGSDARQALTSTLDATAFGSRERVTLAL
                     AHSRHPADGVRWVRRRVPGDHRVRIDGLRCPSASFVAVEASARDEGELIEQFLRERRM
                     NVDSLALALKALAGTPGQDVRRRVVRCGMDNPWSGGERQLQALLRARRVAGWIANSEL
                     IIEGKTYYPDLLWPDERVIVEFDGYQVHTRRDVFETDRTRQNALMLAEYLVLRITWTQ
                     LTEAPDEVVRLIRQALKVGAALSHA"
     gene            complement(8280..9644)
                     /locus_tag="PLF56_02185"
     CDS             complement(8280..9644)
                     /locus_tag="PLF56_02185"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_013862865.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_function: GO:0022857 - transmembrane transporter
                     activity [Evidence IEA];
                     GO_process: GO:0055085 - transmembrane transport [Evidence
                     IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="MFS transporter"
                     /protein_id="HOQ52402.1"
                     /translation="MTDTIQPRKTPAKATIASWVGTTLEYYDFAVYATASALVFNQIF
                     FPPELSEGVRLMLSLATLGVGYLVRPLGAFILGPLGDRYGRKFVMMLTLFGIGGCTFL
                     IGCLPTFAQVGALAPILLVVIRMIQGLSLSGEQAAAITMTLEHSPERRRSFTTSFVTS
                     GSAAGGLLSTAIFIPFVAMPQEQLLSWGWRVPFWLSAVVVVVAYIIRRTLEEPPAFLE
                     AQAVEVQLSPLKQAMQFHWPSILRIAACAMIAGVSYLFATFSVGFATTGYKLDRATML
                     WVPVLASLLALLYTPAAGWLADKIGRKTVFMIGAAGAGLSTAPYLWAITQGNWPLIFA
                     LGILCNGFFYSTANAAWPSFFAEMFPSRVRVSGLAVGTQIGFALSGAIAPVAATALAG
                     GTLKDWVGPSLFACALMLVAVGSAFTAKETRPYTLDEIDQVKQSEREKAAVAAATVSS
                     PVAA"
BASE COUNT         1534 a         3317 c         3518 g         1542 t
ORIGIN      
        1 cgggcccagc ctgcgtaggc cgggccgccc tcccagtagg gcacccggtg cgagtcgcgt
       61 tccacggtgc gaatgtacgg gtcggcgccg gcgtcgacgc gggcggcatc agccgagcag
      121 gcgggcacgc tgagcccgtg ccaatgaatg ttgcgtgcgg acgggccgtg gccggggttg
      181 aagaagcagg gaggacgtcg tgacggcatc ggctcaccgg cggcgcgggc ggccagccgg
      241 gcgaccgtcc aacggccctc ttcgaggttc tcggtgacca gacacaactc gtcgggctcg
      301 gtcgcctcgt gcaggcagcg aaccgcccgc aggcgggccg cctccgcgtg ctgaaactcg
      361 gcctttgcca caggatcgtt ctccgtgacc gcgaccagcc ggttgagggc ctcgaccagg
      421 ccgaccacgt ccgccgccgc cgtacgcttg gccatggcga gctgcgagtc cttttcggcg
      481 cgcgcccggc ggcccttcca cgccttgttc cgggtgatca cgacgaaggt cgcggctacg
      541 gcagccacga tcagtaccgc gccgagaagg tccatccgat cattgtgcac acaaatgctg
      601 ccgtgtcacc aagaaaatcc gatgaattac tctccttgcg acacaagcct gagagaatcg
      661 acccgcagct gcccggtttc tctcagtttg cgtcgtcgat cgtgcaaagc accgcaccgc
      721 tggtgacggt ggcgcccgcc actgccttga ggttggtgat cgtgccggcc cggtgtgccg
      781 agatcggctg ttccatcttc atcgcctcga gcaccacgac caggtcgccc tcggccacct
      841 cgtcgccttc ggccaccgcg acccgcacga tggtgccctg catgggtgcg gtcaggtcgg
      901 cggacgccac cgccgccgac ttgcccgcgc cgcccgatcg acgcggggtg cgcgtgggct
      961 gcgccgagcg cttgaccggt gcgagcagct cgccgggcag gctgacctcg aggcggcgtc
     1021 cgtccacctc gaccacgaag ctctgccgct cggatgcctc gacggcctcg gatgcgccct
     1081 ggtacggggc gatggtgccg gtgaactccg tctcgatcca gcgggtgtgc accccgaacg
     1141 caccgtcggc ggcggtgtag gcgggatcgt tcagcacggc gagctggaac ggcagcaccg
     1201 acggcatgcc ctcgatgcgg gtctcggcca gtgcgcgacg tgagcgctgc agggcctggc
     1261 tgcggtcggc gccggtgacg atgatcttgg ccaccagcga gtcgaacgcg ctgggcacgg
     1321 tcatgccggc caggtagccc tcgtccaccc ggacgcccgg accggccgga ggctgccaca
     1381 cggccagggt gccgggggcc ggcatgaagt tgcggccggc gtcctcggcg ttgagccgga
     1441 actcgatcga gtgggcgcgg gggaccgggt cgtcgtagcc gagggcctcg ccggcggcga
     1501 tgcggaacat ctcgcgcacc agatcgattc ccgtgacctc ttcggacacc gggtgttcga
     1561 cctgcaaccg ggtgttgacc tcgagaaagc tgatggtgcc gtcctggccg accaggaact
     1621 cgcaggtgcc ggcgccgacg tagccggcct cgcgcaggat cgccttgctc gagctgtaca
     1681 ggcgctcgat ctggtcgtcg gtcaggaacg gggcgggggc ctcttcgacc agcttctggt
     1741 gccgccgctg cagcgagcag tcacgcgttg agaccaccac gacgttgccg tgctgatcgg
     1801 ccaggcactg ggtttcgaca tgccggggcc gatcgaggta gcgctcgacg aagcactcgc
     1861 cacgtccgaa cgcggtgacc gcctcacgca cggccgactc gaacagctcg gcgacctcgt
     1921 ccaggttgcg ggcgaccttg aggccgcgcc cgccgccgcc ataggcggcc ttgatcgcga
     1981 tcggcagccc gtgttccttc gcgaacgcga ccacttcatc ggccccggcc accggatcct
     2041 tggtacccgg caccagcggc gcacccacgc gggccgcgat gtgccgcgcc tgcaccttgt
     2101 cgccgaggga ttcgatggcg gccggcgggg gaccgaccca ggtcagcccg gcgtcgatca
     2161 cggcctgggc gaaggccgcg ttctcggcca ggaagccgta accggggtgg acggcctgcg
     2221 ctcccgactt ttcggcgacc gcgaggatct tggcgatgtc gagataggtc tcggccggcg
     2281 tcgtgccgcc cagcgcaaag gcttcatcgg ccagcgcgac gaacagcgag tcggcgtcct
     2341 ggtcggcgta gcaggcgacg ctaccgatgc cggcgtcgcg ggccgcgcgg atcacccgga
     2401 cggcgatctc gccccggttg gcgaccagaa ccttggagaa gagctctccg gatgccatct
     2461 cgacgtcctt tctcgcagtt gttcgtggat gcgtcggtgc cgctcagtgc cggacgctcg
     2521 cccgccaggc ccccgggccg ggccccggcc gggtgcgcag ttgcaggtgc cgggaccgcc
     2581 acccctgcgc gccccgcgcc ggaggctcgt ccgccccagc ctcggcggcg gcagtgcgca
     2641 gcgcgaccag tgcggccgcc agcgcggcca gctcgaggtc ggtgggacgt ccgcggacga
     2701 tgatcggcgc ggtcacagcg gaatgttccc gtgcttcttg ggcggcaggg aggcgcgttt
     2761 ggtgcgtagc agccgcagca ccttggcgat ctcggcgcgg gtgtcacggg gcaggatcac
     2821 ccggtcgacg tagccgcgtt cggccgcgat ccacgggttg gacagctcgt cgttgtaggc
     2881 ctcgatcagc tcggcccggc gggtcaccgg gtcggaactc tcggccagtt cgcgccggta
     2941 caaaatgttc accgcaccct cggcgcccat caccgcgatc tgggccgtgg gccaggccag
     3001 gttcacatcg gcgcccagat gcttcgaacc catcacgtcg taggcgccgc cgtaggcctt
     3061 gcgggtgatc agcgtgacca gcggcaccgt cgcctcggcg taggcgtaca gcagcttggc
     3121 gccacggcgg atgatgccgc tccactcctg gtcggtgccg ggcagaaaac cgggcacgtc
     3181 gaccagggtg agcaccggca cgttgaacgc gtcgcagaac cgcacgaacc gggccgcctt
     3241 ctcggaggcg tcgatgtcga ggcagcccgc gtacaccatg ggctggttgg cgacgacacc
     3301 gaccgggtgg ccctcgaccc gtccgaaacc acagacgatg ttggcggcga agcgggcgtg
     3361 gatctcgcag aactcgccct cgtccagcac ccgggtgatc acctcgtgca tgtcgtaggg
     3421 ctggttcggc gaatccggga tcagggcgtc cagcgcctcg gcgtcggcgg catgctccag
     3481 gtcgagatcg acctcgaggg tgggcggctc gtcgaggttg ttggccggca gaaagctcag
     3541 cacgtgccgg acgaactcga gcgcgtcctg ctcgtcggcg gccaggtagt gggcgttgcc
     3601 ggacttggtg ttgtgggtgc gggcgccgcc cagctcttcc atcgacacct cttcgccggt
     3661 gacggtgcga atcacgtccg gcccggtgat gaacatctgc gaggtctggt cgaccatcac
     3721 caccacgtcg gtgagcgcgg gggagtagac gtgacccccg gccgcggcac ccatgatcac
     3781 cgagatctgc gggatcaccc cggacgcatg ggtgttgcgt ttgaagatct cggcgtagag
     3841 gccgaggctg accacaccct cttgaatgcg ggcgccgccg ccctcgacca gaccgatgat
     3901 cggcaccccg gtacgcatcg cgaggtccat gatcttgcag atcttctcgc cgtacacctc
     3961 acccagggca ccgccgaaga tcgtcacgtc ctggctgaac acgcacacgt tgcggccgtc
     4021 gatcgtgccc atgccggtga tcacaccgtc ggtgtagggg cgcttggcgt ccatgccgaa
     4081 ggccgtggtg cggtgccggg cgaactcgtc caactcggtg aaactgtcgg ggtcgagcag
     4141 ggccagcacc cgctcgcgcg cggtcagctt gccgcggtcg tgaatcttgg cgacggcggc
     4201 gtcggacgcg gggtgcttcg ccttctcgtt gcggcgctgg aagtcggcga gcttgccgga
     4261 ggtcgtgtgg atgtcgaact ccatatccga acactagccg tcccggcgag gtcgcgatgc
     4321 cagtttcggg gtgggcagat gcgatgggtc ggctgaccgg gcagcagcgc gcttcatggt
     4381 gcggggcgat ggcgcaggcg cgggcgcctg ccttgtcgcg aggtgaaaca tggggccgcc
     4441 cgccggcgct gtgggccggg gtgcactccc cggacgatcc ggccggtcga taaccgcggc
     4501 ttatgacgga acgcacattt cgtcttaacc accgcgcagt cggcgctctt agcatcgaag
     4561 ccaccagcca acagatgaga gaaccgatga ctgccgaagc cgccagaatg agccgcgtcc
     4621 agccgtcccc gagcacggtc gccgccggac gtgcgcgcga gcttcgcgag cagggcgttg
     4681 cggtgctcga cctgaccgtc ggtgagcccg acttcgacac ccccgacaac gtgaaggcgg
     4741 cgggcatcgc cgcgatcgag cggggcgaga ccaaatacac cgcggtgaac ggcaccgccc
     4801 cgctgcgccg ggccatcatc gatcggctgc ggcggctgac cggcctgacc tacaccgacg
     4861 ccgagatctc ggtcggctcc ggcgccaagc acgtgatctt caccgccctg atggccaccc
     4921 tcagcccggg cgacgaggtg gtcatccccg cgccgtactg ggtgtcgtac cccgacatgg
     4981 tggtgctcaa cgacggtgtg ccggtggtcg tgccctgccc gatcgaggac gacttcaagc
     5041 tcacccccga tcggctccgc gaggccctga ccgggcgcac ccgctggctg atcctgaacg
     5101 cgccgtccaa cccgaccggt gcggtctacc gcccggacga gttgcgcgcc ctggctgagg
     5161 tgctgcgcgc ctttccgcag gtactggtgc tgaccgacga gatctacgac gaggtgtttc
     5221 tcggtgcggg acgctgcacc gggctgctcg aggtcgctcc cgacctgcgt gagcgggtgc
     5281 tgatcgtcaa cggtgtctcg aagacctacg cgatgaccgg ttggcgactg ggctactgcg
     5341 ccggaccgtc cgctgtggtg ggggcgatca acaagctgca gtcgcacatg gcgtcgtgtc
     5401 cctcgtcgat ctcgcaggcg gccgccgtcg aggcgctgag cggcgaccag tcgttcgttg
     5461 cggacgccgc cgcccagtac cgccgccgac gcgacctggt ggtcgagcgg gtcaacgccg
     5521 tcgacggcct gcgtgcactg gccggcgacg gtgcgttcta cgtgttcgtc gactgctcgg
     5581 gcgtgatcgg ccggcatacc ccgtccggtg tcgtaatcga cagcgacgac gccttcgtcg
     5641 ggtacctgct cgatcaggcc cgcgtggcca ccatcaccgg cagtgcctac gggctgtcgc
     5701 cgttcttccg cgtctcgttc gccaccagcg aggccgtcct gatcgccgcg atggacgcca
     5761 tcgccgcggc cgtcgcccaa ctccagtgag ccgatcgtta ccagtgaaga ggtctgagcc
     5821 atgatccacg tccgcacccg catcgagcgc cccgaccccg cgctggtcgc cgggctggcg
     5881 cagtactcga gcgcgaccat ccacgaggcc cagggacgcc gcggcgcgct cgactcgtcg
     5941 ctgaagccga tcgaccgctc gatgtcgttc tgcggtccgg ccgtgaccgt ggtctgtgcg
     6001 ccgcgcgaca acctgatgct gcaggtggcc atccactacg cgcaacgcgg tgacgtgctg
     6061 gtcgtctcgg ccggcgagtt cggcgaggcg ggcaccttcg gcgacgtgct gggcacggcc
     6121 atgcactcca aggggctggc cgcgctggtc accgactcgg gcgtgcgcga caccgccgac
     6181 ctgatcgacc tgggcctacc ggtgttctcg cgcagcgtct gcatcaaggg cacggtcaaa
     6241 gagacgctgg gtccgatcaa ccatccgatc gtgttcggcg gcgaactggt cgagccgggc
     6301 gacatcgtca aaggcgacgc ggacggggtg gtgctggtca aggccgccga cgccggctgg
     6361 gtgatcgagg cctgcgccga gcgcgaggcg tccgaggccg aactgcgcga gctctaccgg
     6421 gccggcggca ccaccatcga gttgtgcaac ctggtcgaga agctggcggc caaaggcttg
     6481 ctggtcgagg agtagagcgg acgtccggct cgcgccatgt gcgaggacgg ggcgcgcgaa
     6541 actgcaccac atttgtggag tggctcgcct cacagaggcg gcgaggccgc ggaatgtggt
     6601 gcagtttcgc acgtcaccgc cgggcctggc gttcgagaac cgtccccaac cccggccacg
     6661 ccgctggcgg gagtggagaa ccgtccccaa ccccggccat gccgctggcg ggagtagaga
     6721 accgtcccaa ccccggccgc gccgctggcg ggagtggaga accgtcccca accccggcca
     6781 cgccgctggc gggagtggag aaccatcgcc aaccccggcc acaccgctgg cgggagttgg
     6841 agaggatctg caccacattt gtggcgtggc tcgcctcacg gaggcggcgg gctgcggaat
     6901 gtggtgcagt ttcgggcgaa gcccgcggcc ctttggcctg tggataacgc cccacgggcg
     6961 cgcatggcct gctggactcg ctccatggac tccgatgacc tggtcatgct gatgcatgtg
     7021 atgtcgctga acaatggcgt actgcgacct cgcgaccacc cgaactcacg cgcgaccctc
     7081 gcccgcttgg tgcgcagcgg cgcgatcgtc aaggtgctgc ccggcaccta cgtggacgcc
     7141 gatcgctgca cggagcgtcg aacccggtat gccgccgcgc tggccttcgc tcccggatcg
     7201 gtgctctggg ggtccgatgc ccggcaggcg ctcaccagca cccttgacgc aaccgcgttc
     7261 ggctcacgcg agcgggtgac acttgcgctc gcccacagcc ggcaccctgc ggacggcgtc
     7321 cgctgggttc ggcggcgggt gccgggcgac caccgcgtcc ggatcgatgg gttgcgctgc
     7381 ccgtccgcga gcttcgtggc cgtcgaggcc tccgcgcgcg atgagggtga gctgatcgag
     7441 cagtttctgc gcgagcgacg catgaacgtc gattcgctcg cattggcgct gaaggccctg
     7501 gccggtacac cggggcagga cgtgcgacga agagtcgtcc gatgcggcat ggacaatcca
     7561 tggtcgggtg gcgaacggca gttgcaggcg ctgctgcggg ctcgccgggt ggccggctgg
     7621 atcgcgaaca gcgaactgat catcgagggc aaaacgtatt acccggacct gctctggccc
     7681 gacgagcggg tgatcgtgga gttcgacggc taccaggtac acacccggcg cgacgtcttc
     7741 gagaccgatc gcacccggca gaacgcgttg atgctggccg aatacctggt gcttcggatc
     7801 acgtggaccc aactcactga ggccccggac gaagtcgtcc ggctgattcg ccaggcactg
     7861 aaggtcggcg cggcgctgtc ccacgcatga tgagtgtgtg aatctgcacc agattcgcgg
     7921 gggcgtgcgg ctcggtgagc gtgatcgagg cccgaatgtg gtgcggttct gcacagctcc
     7981 acccggggga gggcggagtc cggacgctgc gcaacccgcg accccggctg gacgcggccg
     8041 gacgcttccc tggcccacgc gtgatgagtg tgcgaatctg caccagattc ttgggagcgt
     8101 gcggctcggt gagcgtaacc gagggtcgaa tgtggtgcgg ttctgcacag gtcacccggg
     8161 gagggcggtt ggcatccgga cgttgcgcgc ccgcgactcc ggccggacgc tgccctggcc
     8221 ggcgagcgtc ggccagggcg cgatcgtcca ctggggtcag gaagacaaaa cgttgagcct
     8281 cacgccgcca cgggcgagga caccgtcgcc gcagccaccg ccgccttttc gcgttcgctc
     8341 tgcttgacct gatcgatctc gtccagcgtg tacggtcggg tctccttggc ggtgaacgcc
     8401 gaacccacgg ccaccagcat cagggcgcag gcgaacagcg aggggccgac ccagtctttc
     8461 agcgtgccgc cggccagcgc ggtcgcggcc accggggcaa tcgcaccaga caaggcgaag
     8521 ccgatctggg tgccgaccgc gagccccgag acgcgcaccc ggctcgggaa catctccgcg
     8581 aagaacgacg gccaggcagc gttggcggtg gagtagaaga atccgttgca gagaatgccg
     8641 agcgcgaaga tcagcggcca gttgccctga gtgatcgccc acaggtaggg cgcggtggac
     8701 aggcccgccc cggcggcgcc gatcatgaac acggtcttgc ggccgatctt gtcggccagc
     8761 cacccggcgg ccggcgtgta cagcagggcc aacaggctgg ccagcacggg aacccacagc
     8821 atggtcgccc gatccagctt gtaaccggtg gtggcgaagc cgaccgagaa ggtggcgaac
     8881 aggtagctga cgccggcgat catggcacac gccgcgatcc gcaggatcga gggccagtga
     8941 aactgcatgg cctgcttgag cggggagagc tgcacctcga cggcctgcgc ctcgaggaaa
     9001 gccggcggct cttcgagcgt gcgccggatg atgtaggcga cgacgaccac cacggccgag
     9061 agccagaacg gaacccgcca gccccagctc aacagttgct cctggggcat ggcaacgaac
     9121 ggaatgaaga tcgcggtcga cagcaggcca ccggcggcgg aaccgctggt cacgaagctg
     9181 gtggtgaacg agcggcgccg ctccggtgag tgctcgaggg tcatcgtgat ggcggcggcc
     9241 tgctcgcccg aaagcgacaa accctgaatc atcctgatca ccacgagcag aatcggagcc
     9301 agcgcgccga cctgggcgaa ggtgggcaga cagccgatca ggaaggtgca gccgccgatg
     9361 ccgaacaggg tgagcatcat cacgaacttg cgcccatagc gatcgccgag cgggcccagg
     9421 atgaatgcgc ccagcggacg gaccaggtag cccaccccca gggtcgccag gctcagcatc
     9481 agccggacgc cctcggacag ttcgggtgga aagaagatct ggttgaagac cagcgccgac
     9541 gcggtggcgt acaccgcgaa gtcgtagtac tccagcgtgg tgccgaccca ggaggcgatg
     9601 gtcgccttgg ccggtgtctt gcggggttga atggtgtcgg tcatttcgcc tccatgggca
     9661 tgtgctcgtt acgtcgcttc ggagaccccg tcagacgggg acttgggcaa cggacggagc
     9721 gggcgggtgg aacgggtagt ggtgctgcgg tcggccgatt gaggtcgaga tccttgatcg
     9781 gccggacggg gcctggttcg agcggcgatg ctcaggtggt gtggccgtcc ggtcgggtgg
     9841 tggtgtggcg gttcggttgg gtgttggtgt gggcggttgg gaagagcggt tcggtcggtg
     9901 ttggtgtggg c
//
LOCUS       DAOSUG010000036         9824 bp    DNA     linear   ENV 10-JUN-2024
DEFINITION  MAG TPA_asm: Micropruina sp. isolate AMR_MDS_2195
            NODE_3786_length_9824_cov_4.398301, whole genome shotgun sequence.
ACCESSION   DAOSUG010000036 DAOSUG010000000
VERSION     DAOSUG010000036.1
DBLINK      BioProject: PRJNA925477
            BioSample: SAMN32864262
            Sequence Read Archive: SRR4236673
KEYWORDS    WGS; ENV; Metagenome Assembled Genome; MAG; Third Party Data; TPA;
            TPA:assembly.
SOURCE      Micropruina sp. (wastewater metagenome)
  ORGANISM  Micropruina sp.
            Bacteria; Bacillati; Actinomycetota; Actinomycetes;
            Propionibacteriales; Nocardioidaceae; Micropruina.
REFERENCE   1  (bases 1 to 9824)
  AUTHORS   Abdulkadir,N., Saraiva,J.P., Zhang,J., Stolte,S., Gillor,O.,
            Harms,H. and Rocha,U.
  TITLE     Genome-centric analyses of 165 metagenomes show that mobile genetic
            elements are crucial for the transmission of antimicrobial
            resistance genes to pathogens in activated sludge and wastewater
  JOURNAL   Microbiol Spectr 12 (3), e0291823 (2024)
   PUBMED   38289113
REFERENCE   2  (bases 1 to 9824)
  AUTHORS   Abdulkadir,N.
  TITLE     Direct Submission
  JOURNAL   Submitted (24-MAR-2023) Environmental Microbiology, Helmholtz
            Center for Environmental Research -UFZ, Permoserstr 15, Leipzig
            04318, Germany
COMMENT     The annotation was added by the NCBI Prokaryotic Genome Annotation
            Pipeline (PGAP). Information about PGAP can be found here:
            https://www.ncbi.nlm.nih.gov/genome/annotation_prok/
            
            ##Genome-Assembly-Data-START##
            Assembly Method        :: SPAdes v. 3.11.1
            Genome Representation  :: Full
            Expected Final Version :: Yes
            Genome Coverage        :: Not Applicable
            Sequencing Technology  :: Illumina MiSeq
            ##Genome-Assembly-Data-END##
            
            ##Genome-Annotation-Data-START##
            Annotation Provider               :: NCBI
            Annotation Date                   :: 05/04/2023 04:57:10
            Annotation Pipeline               :: NCBI Prokaryotic Genome
                                                 Annotation Pipeline (PGAP)
            Annotation Method                 :: Best-placed reference protein
                                                 set; GeneMarkS-2+
            Annotation Software revision      :: 6.5
            Features Annotated                :: Gene; CDS; rRNA; tRNA; ncRNA
            Genes (total)                     :: 2,776
            CDSs (total)                      :: 2,750
            Genes (coding)                    :: 2,714
            CDSs (with protein)               :: 2,714
            Genes (RNA)                       :: 26
            tRNAs                             :: 24
            ncRNAs                            :: 2
            Pseudo Genes (total)              :: 36
            CDSs (without protein)            :: 36
            Pseudo Genes (ambiguous residues) :: 0 of 36
            Pseudo Genes (frameshifted)       :: 2 of 36
            Pseudo Genes (incomplete)         :: 32 of 36
            Pseudo Genes (internal stop)      :: 2 of 36
            ##Genome-Annotation-Data-END##
FEATURES             Location/Qualifiers
     source          1..9824
                     /organism="Micropruina sp."
                     /mol_type="genomic DNA"
                     /submitter_seqid="NODE_3786_length_9824_cov_4.398301"
                     /isolate="AMR_MDS_2195"
                     /isolation_source="Activated sludge of municipal
                     wastewater treatment plants"
                     /db_xref="taxon:2737536"
                     /environmental_sample
                     /geo_loc_name="Canada"
                     /lat_lon="49.25 N 123.1 W"
                     /collection_date="2015-06-16"
                     /metagenome_source="wastewater metagenome"
                     /note="metagenomic"
     gene            complement(<1..4031)
                     /gene="dnaE"
                     /locus_tag="PLF56_02190"
     CDS             complement(<1..4031)
                     /gene="dnaE"
                     /locus_tag="PLF56_02190"
                     /EC_number="2.7.7.7"
                     /inference="COORDINATES: protein motif:HMM:TIGR00594.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_component: GO:0009360 - DNA polymerase III complex
                     [Evidence IEA];
                     GO_function: GO:0003887 - DNA-directed DNA polymerase
                     activity [Evidence IEA];
                     GO_process: GO:0006260 - DNA replication [Evidence IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="DNA polymerase III subunit alpha"
                     /protein_id="HOQ52403.1"
                     /translation="MSDPFVHLRVASGYSLRYGASQPSALVDAAADADMDTLAITDRD
                     GLYGAVKFARACQRTGIAPVVGVDHTMADGSRAVSLATSRQGWASLCRLTSAVHLAGE
                     RGKPRCTPEAMAGHDDLIVMLGDDSDLGRTLATGSTDEAERVLRGWLNLVDRHRLALA
                     VTNHHTPGRGRGSAALAGRMLGFADAHGLTGVLTNAVRMAGKHLAPTIDVLDASRRLV
                     PLDLRNIDRRNAEGYLKTGKEMLAAADEVARLAGRRDAETLIGDTRMLATRCRLDPQA
                     DIGLGEIHLPEFDVLDVRGSAPDAMAALRLRCDAGIAARYGTSSPAIEARLADELTVI
                     DTLGFATYFLTVAEVVAMIGRLGIRCAARGSGAGSLVNYLLGISGVDPIRYGLIMERF
                     LSPLRQVLPDIDIDVESARRTEIYDAILNVFGGDRVACVAMMETYRVRHAIRDVGAAL
                     SLPPREIDAIAKAFPHIRAADARAALRDLPELRAAGLGERRLDVLFSLVESLDGLPRH
                     VALHPCGVLLSDTTLLDRTPVEASFGGFPMSQFDKDDVEELGLLKLDVLGIRMQSAMA
                     HALGEIRRVEGVEVDVDVLAPYDDPDTYDMIGKSVTLGCFQIESPGQRELVGKFGPRD
                     FSDIIIDISLFRPGPVKSEMVVSFLEARQGWKTAHYLHPDLTPALAETGGVVVFHEQV
                     IQIIAIMTGCTLAQGDEARRALGRRDSQADVKRWFVPTCLGRGYSQAVVDRAWFVLES
                     FASFGFCKAHAAAFALPTYQSAWLKRHYPAHFLSGVLTHDPGMYPKRLILEEARRCGI
                     AVLGLDVNVSDDAYRVERLDESVTPGESVGPGESVIPAESVIPAESVIPADAVDPGEP
                     VIPANAGISVERVETPLPPSTSAVSVPRHPGECRDLSQPPSVGLVETTDRVGERSRGK
                     PGMTQDCIIPADAGPSIPGHPAGGFQPDLPVRGRRLSPGDGIPDGRGYGIRLSLADVK
                     GITEAEIARIVAGRPYYSLTDFWQRTGNPSDITERLALAGAFDALYGIDAPSAVRPRG
                     QITRRDLLLAVADLERADRAHARAHGRARGLRAPRSADPVGDPRELARRQAQNAKPVA
                     PQEIQLPLDFTESLGDRELDEHGLDRLDRWDGPGGFDGRDGPGGFDGRDGPDRFDGRD
                     GADRFDRRRWPSDVTPTGLTELSDAERLSAELEIIGLDASKHLLTGYLPFLDAVGVTF
                     AADLLSRRSRGEVMIAGVKVATQTPPVRSGRRVVFLTVDDSTGPADATFFEDAQGPYA
                     ATVFSSWLLLVRGELRRTGPRGVSVRATGAWDLTLLHNTHTAAFTETGDPDQALQRVR
                     DLMAEVPDGFGVGDAEGRRRVLV"
     gene            complement(4031..4411)
                     /locus_tag="PLF56_02195"
     CDS             complement(4031..4411)
                     /locus_tag="PLF56_02195"
                     /inference="COORDINATES: protein motif:HMM:NF037499.2"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="SAV_6107 family HEPN domain-containing protein"
                     /protein_id="HOQ52404.1"
                     /translation="MTRSELVAAASALAAAELAEQPAQRYLGAHLAALRVAAVVLACR
                     AASTRSGSRPRDVWQVLAEAAPEYAEWAGFFASSALKRRAVQAGAVALVSAREADDLL
                     RDVRLFHDQVARRLWAHATERVAI"
     gene            complement(4411..4857)
                     /locus_tag="PLF56_02200"
     CDS             complement(4411..4857)
                     /locus_tag="PLF56_02200"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_012920488.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="DUF6504 family protein"
                     /protein_id="HOQ52405.1"
                     /translation="MRRYNEAIEVRQGLVGDDEGPAQFLWRHRLWKVLELQARWIETA
                     DWWNSAQVRAARGETPDVRGTTPGLGAPGSRAGGSAPGSRMGGSAPGFRMVGSGGVGI
                     DDGDDLLGEQEVWRVVAAEGRYGTSGVYELARSWGSGAWRLRTVVD"
     gene            5358..6416
                     /locus_tag="PLF56_02205"
     CDS             5358..6416
                     /locus_tag="PLF56_02205"
                     /inference="COORDINATES: protein motif:HMM:NF012742.2"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_function: GO:0000976 - transcription cis-regulatory
                     region binding [Evidence IEA];
                     GO_function: GO:0003700 - DNA-binding transcription factor
                     activity [Evidence IEA];
                     GO_function: GO:0043565 - sequence-specific DNA binding
                     [Evidence IEA];
                     GO_process: GO:0006351 - DNA-templated transcription
                     [Evidence IEA];
                     GO_process: GO:0006355 - regulation of DNA-templated
                     transcription [Evidence IEA];
                     GO_process: GO:0045892 - negative regulation of
                     DNA-templated transcription [Evidence IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="LacI family DNA-binding transcriptional
                     regulator"
                     /protein_id="HOQ52406.1"
                     /translation="MTRVTMKDVADVMGVSVMTVSNAFNRPDQLSGELRDRILNRARK
                     MGYGAPNAAARNLRQGRTNCFGVAFSESLTYAFSDPYSVLWLSGLSGALEQAGKTMML
                     LPIAEPTETNLDLIRRASVDGIAGLCVSSQVRTVAQQTGMPLVCTDPADPDGSWVTID
                     DHAAGRLVGQHIRRLGHHRVALVVEQAVPLREAEPAQQSMATYRAELDDYRTLGLYDL
                     WGRVEGFLAELDGLDVQVVSVGLNSRQSGRAAAELLLDQLARPTAIICVSDVIAFGVL
                     DAMHDRGLDPGRDVTVTGFDDLPEAGRLGLTTVHQPIQEKGRLVGELLLDPTRTPRRI
                     TLPHQLIVRSSSAPPHGS"
     gene            6757..6969
                     /locus_tag="PLF56_02210"
     CDS             6757..6969
                     /locus_tag="PLF56_02210"
                     /inference="COORDINATES: ab initio
                     prediction:GeneMarkS-2+"
                     /note="Derived by automated computational analysis using
                     gene prediction method: GeneMarkS-2+."
                     /codon_start=1
                     /transl_table=11
                     /product="hypothetical protein"
                     /protein_id="HOQ52407.1"
                     /translation="MYLVLNSTDAARALNGSARPDAPVIGELASKARRDLLGDLALRV
                     SLAGERLTRRLRRNGRAGHAAALSHS"
     gene            7164..7754
                     /locus_tag="PLF56_02215"
     CDS             7164..7754
                     /locus_tag="PLF56_02215"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_020573413.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="DUF4126 domain-containing protein"
                     /protein_id="HOQ52408.1"
                     /translation="MEVLPLAFTGGWASGINAWATVLMLGLLGRFAGVEGVPAGFERT
                     DVLIVMAVLAVAELVADKIPYLDSAWDAVSTFIRPIAGATIGALIAGHTGDLGTMALA
                     AVGGVTALLSHLAKSSLRLAINTSPEPVTNLTASAAGDLSLVGVLALAALNPVAAATI
                     AAALLVLTLGLALFLLNRVGRGWRAFRVWLARRGRA"
     gene            7751..9043
                     /locus_tag="PLF56_02220"
     CDS             7751..9043
                     /locus_tag="PLF56_02220"
                     /inference="COORDINATES: protein motif:HMM:NF013434.2"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_function: GO:0016491 - oxidoreductase activity
                     [Evidence IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="FAD-dependent oxidoreductase"
                     /protein_id="HOQ52409.1"
                     /translation="MTVIAVVGGSPAGMAAAARLAKQRHRVLLFEASDRLGGRWAQPG
                     ALPPVLSLPAPWRDLFRKSGRAFDVELARTGLALVAAAPAVHRFADGSELTLPTDRGE
                     QWTTLTAAWGTPAATRWRDLLDDLDQRWQLLRRLGLEDEFTEVALTPARRRALGLDRT
                     VEKLARDFGHPQAAALIRSTAWRFGSKPARTPAFVAVRLAIDRTFGRWQLTRDGHPVP
                     ASALIDLLADRLTLRGVEVHSGRPVTALAPGRVRTADGDIVVDAIVAAVNPWTYLRLT
                     GTQERVLSRRTAWTRPALAPRITVDQEDSVSSGLEYQAHSHTVIHEIRETVQHSAVGG
                     WVDYRLPSPTGILTVRHDFSDPVPDPGFGTRWTSPRTWLRQPPLRAATPTVVTASASS
                     RGGNDPWAQLLSGALAAYVVHENLTGADIRPTNKAARP"
     gene            complement(9185..9556)
                     /locus_tag="PLF56_02225"
     CDS             complement(9185..9556)
                     /locus_tag="PLF56_02225"
                     /inference="COORDINATES: protein motif:HMM:NF019656.2"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="DUF1707 domain-containing protein"
                     /protein_id="HOQ52410.1"
                     /translation="MDNLPISSKYRSRPLDPLDDAEREQLAERLNAEYAKGGMDTDTY
                     RAMLDQVFASQTLGQVAEVAAALPGRDTFAVPAVIETGTVAPGELVPAKGPGNKGALM
                     LVAGLGTGIAVAILLLVLIIL"
BASE COUNT         1488 a         3593 c         3311 g         1432 t
ORIGIN      
        1 tgcaccagca cccgacgccg gccctcggcg tcgcccacgc cgaacccgtc cggcacctcg
       61 gccatcaggt cacgcacccg ctgcaaagcc tggtcggggt caccggtttc ggtgaacgcg
      121 gcggtgtggg tgttgtgcag cagggtgagg tcccaggcgc cggtggcccg caccgacacc
      181 ccgcgcgggc cggtgcggcg cagctcgccg cgcaccagca gcagccacga tgagaacacg
      241 gtggcggcgt aggggccctg ggcgtcttcg aagaaggtgg cgtcggccgg cccggtggag
      301 tcgtcgacgg tgagaaagac cacccgccgt ccggaccgca ccggtggggt ctgggtggcc
      361 accttcaccc ccgcgatcat cacctcgcca cggctgcgcc ggctgagcag atcggcggcg
      421 aaggtgacgc cgacggcatc caggaacggc agatagccgg tgagcaggtg cttgctcgcg
      481 tccaggccga tgatctcgag ttcggcgctg agtcgctcgg catcgctcag ctcggtcagt
      541 ccggtgggcg tgacgtcact gggccaccgc cgtcggtcga acctgtcggc accatcccgc
      601 ccgtcgaacc tgtcgggacc atcccgcccg tcgaacccgc cgggaccatc ccgcccgtcg
      661 aacccgccgg gaccatccca ccggtcgagc ctgtcgagac catgctcgtc cagttcccgg
      721 tcgccgaggc tctcggtgaa gtcgagcggc aattggatct cttgcggggc gaccggcttg
      781 gcgttctgcg cttggcggcg ggcgagttca cgggggtcgc cgaccgggtc ggccgagcgg
      841 ggtgcacgaa gcccgcgtgc acgcccatgg gcgcgggcgt gggcacggtc ggcacgttcc
      901 aggtcggcga cggcgagcag caggtcacgc cgggtgatct ggccgcgagg ccgcaccgca
      961 cttggtgcgt cgatgccgta cagggcgtcg aaggcgccgg ccagcgccag ccgttcggtg
     1021 atgtcggacg ggttgccggt gcgttgccag aaatcggtca gcgaatagta gggccggccg
     1081 gcgacgatgc gggcgatctc ggcctcagtg atgcccttca catcggccag ggacagccgg
     1141 atgccgtagc cgcgtccgtc cggaatgcca tcgccggggc tcagccgcct gccccgcact
     1201 ggcaggtcgg gttggaaccc gcccgccggg tggccgggta tcgacggccc ggcatccgcc
     1261 ggaatgatgc agtcctgcgt catcccgggc ttgccccggg atctctcccc cacccgatca
     1321 gtggtctcga caagcccgac cgacggtggt tgcgagagat cccggcattc gccgggatga
     1381 cgaggcacgc taacagcgga agtggaaggt ggcagagggg tctcgacacg ctcgaccgag
     1441 atcccggcgt tcgccgggat gacgggctcc cccgggtcga cggcgtccgc cgggatgacg
     1501 gactccgccg ggatgacgga ctccgccggg atgacggact cccccgggcc gacggactcc
     1561 cccggggtga cggactcgtc caaccgttcg acccggtacg cgtcgtcgct gacgttcacg
     1621 tccagcccca ggacggcgat gccgcaccgc cgcgcctcct ccaggatcag ccgcttcgga
     1681 tacatgcccg ggtcgtgggt gagcactccc gacaggaaat gcgccgggta gtgccgcttc
     1741 agccaggccg actgataggt gggcagcgcg aatgccgccg catgcgcctt gcagaagccg
     1801 aaggaggcga acgactccag cacgaaccag gcccggtcaa ccacggcctg ggagtaaccc
     1861 cgacccagac acgtgggcac gaaccagcgc ttcacgtcgg cctgggaatc gcgccgaccg
     1921 agcgcccgcc tggcctcgtc gccctgggcc agggtgcagc ccgtcatgat cgcgatgatc
     1981 tggatcacct gctcgtggaa caccaccacc ccgccggtct cggccagcgc cggcgtcagg
     2041 tcggggtgca ggtagtgggc ggtcttccag ccctgccggg cctcgaggaa ggacaccacc
     2101 atttcggact tcaccgggcc ggggcggaac agcgaaatgt cgatgatgat gtcggagaag
     2161 tcgcgcggcc cgaacttgcc gaccagttcg cgctgcccgg gcgattcgat ctggaagcaa
     2221 cccagggtga ccgacttgcc gatcatgtcg taggtgtcgg ggtcgtcata gggggcgagc
     2281 acgtcgacgt cgacctcgac gccctctacc cggcggatct cgcccagcgc gtgcgccatc
     2341 gccgactgca tgcggatgcc gagcacgtcc agcttgagca ggccgagttc ttcgacgtcg
     2401 tccttgtcga actggctcat cggaaagccg ccgaagctgg cctcgacggg ggtgcggtcg
     2461 agcagcgtgg tgtcgctgag cagcactccg cacggatgca gcgccacgtg ccggggcagc
     2521 ccgtccagcg attcgaccag agagaacagc acgtcgagcc gacgctcgcc cagcccggcg
     2581 gcacgcaact ctggcaggtc gcgcagggcg gcgcgggcgt ccgcggcccg aatgtgcgga
     2641 aacgccttgg caatggcgtc gatctcgcgc ggcggcaggc tgagcgccgc acccacgtcg
     2701 cgaatggcgt gccggacgcg gtaggtctcc atcatcgcca cgcaggccac ccggtcaccg
     2761 ccgaacacgt tcaggatcgc gtcatagatc tcggtacgcc gggccgattc gacgtcgatg
     2821 tcgatgtcgg gcaggacctg ccgcagcggc gacaggaagc gttccatgat cagcccgtag
     2881 cggatcgggt cgacaccgga aatgcccagc agatagttga ccagcgagcc ggcgcccgag
     2941 ccacgcgcgg cgcagcgaat accgagccgg ccgatcatcg ccaccacctc ggcgacggtg
     3001 agaaagtagg tggcgaagcc gagggtgtcg atcacggtca gctcgtcggc cagtcgggcc
     3061 tcgatcgcgg ggctggacgt gccgtagcgg gccgcgatgc cggcgtcgca gcgcagcctc
     3121 agcgcggcca tcgcgtcggg tgccgaaccg cggacgtcga gcacgtcgaa ctcgggcaga
     3181 tggatctcgc ccagtccgat gtcggcctgc gggtcgagcc ggcacctggt ggccagcatc
     3241 cgggtgtcgc cgatcagcgt ctcggcgtcg cgccgtccgg ccagccgggc gacctcgtcg
     3301 gcggcggcca gcatttcttt gccggtcttg aggtagccct cggcgttgcg ccggtcgatg
     3361 ttgcgcaggt cgagcggcac cagccggcgc gaggcgtcca gcacgtcgat ggtgggcgcc
     3421 aggtgcttgc cggccatgcg gacggcattg gtcagcaccc cggtgaggcc gtgggcgtcg
     3481 gcgaagccca gcatgcgacc ggccagcgcg gccgagccgc ggccacgtcc gggggtgtga
     3541 tggttggtca ccgcgagcgc cagccggtgc cggtcgacca ggttcagcca gccgcgcagc
     3601 acgcgttcgg cctcgtcggt cgaaccggtg gcgagggtgc gccctagatc ggagtcgtcg
     3661 cccagcatga cgatcaggtc gtcgtggccg gccatcgcct cgggggtgca gcgcggcttg
     3721 ccgcgctcac cggccaggtg caccgccgag gtcagccggc acaacgaggc ccagccctgc
     3781 cgcgaggtcg ccaggctgac cgcgcgggag ccgtcggcca tggtgtggtc gaccccgacc
     3841 accggggcga tgccggtgcg ctggcaggcc cgggcgaact tgaccgcgcc gtagagaccg
     3901 tcacggtcgg tgatcgccag ggtgtccatg tcggcgtcgg cggccgcgtc gaccagggcc
     3961 gacggctggg acgccccgta gcgcagcgaa tagccggagg cgacccgcag gtgcacgaac
     4021 gggtcgctca tcagatcgcc acccgttctg tcgcgtgcgc ccacagccgc cgcgccacct
     4081 ggtcgtgaaa gagccggacg tcgcgcagca ggtcgtccgc ctcgcgggcg ctgaccagag
     4141 cgaccgcacc ggcctggacg gcccgccgtt tgagcgcaga ggaagcgaag aagccggccc
     4201 attcggcgta ctcgggggcc gcctcggcga gcacctgcca cacatcacgc ggacgcgacc
     4261 cgctccgggt ggacgccgcc cggcatgcca gcaccaccgc cgccactcgg agtgcggcca
     4321 ggtgggcgcc gaggtagcgc tgtgccggct gctctgccag ttcggccgcg gccagcgcgg
     4381 acgccgccgc gacgagttca ctgcgcgtca tcagtccacc accgtgcgca gccgccacgc
     4441 cccgctgccc caggacctgg ccagttcgta gaccccggac gttccgtagc ggccctcggc
     4501 cgcgaccacc cgccagacct cctgctcgcc gagcaggtcg tcgccgtcgt cgatgccgac
     4561 accacctgac ccgaccatgc ggaagcccgg cgcggacccg cccatgcggg agcccggcgc
     4621 ggacccgccg gcgcgggagc ccggcgcacc gaggccggga gtcgtgccgc ggacgtccgg
     4681 cgtctcaccc cgggcggccc gcacctgggc ggagttccac cagtcggcgg tctcgatcca
     4741 acgggcctgc aactcgagca ccttccacaa ccggtgccgc cacaggaact gcgccgggcc
     4801 ttcgtcatcg cccaccagac cctggcgaac ctcgatcgcc tcgttgtatc tgcgcatgct
     4861 gaaacccccg tggacacggc gtggaaacca ttcgccgggg gaatgcatga ccccggtgcg
     4921 gacgggggtc gagatccgtc cgcaccgggg agctatgagc ccttccccga ctcgatatcg
     4981 aacatctgtt cgactcgatc cagtctgcct gttcggttcc ggtccgtcaa ggggcggctt
     5041 ggaaacctcg aactccaggg cggaggattc accatcaccc gatggcgacg tccgctcgaa
     5101 gagcgttcgt cgcccagtct cgccccgcag cccacgcatc ggcatcaggc acgccgcacg
     5161 cccaccccgt ccacgcgcac accatcagcc cgctccgccg accgcgccgc cccgatcctc
     5221 gatccggcac gaccctggac gccccgatcc gcaccgatca actttaacgt tagagttcgc
     5281 cgttctggtt gctctctgta tcgttaaagt ccatcatgga taccagaagc agttccgctg
     5341 aaccgaatga ggtggacatg acccgcgtca ccatgaaaga cgtcgccgac gtgatgggcg
     5401 tatcggtgat gaccgtctcg aacgcgttca atcgccccga ccagctctcg ggcgagctgc
     5461 gcgaccgcat tctgaaccgg gccaggaaga tgggctacgg cgcacccaac gccgccgccc
     5521 gcaacctgcg tcaggggcgc accaactgct tcggcgtggc gttctcagaa agcctcacct
     5581 acgccttcag cgacccctat tcggtgctgt ggctgagcgg cctgagcggc gcgctggagc
     5641 aggccggcaa gaccatgatg ctgttgccga tcgccgaacc gaccgaaacc aacctcgacc
     5701 tgatccgccg cgccagtgtg gacggcatcg ccgggctctg cgtctccagc caggtgcgca
     5761 cggtggcgca gcagaccggc atgccgctgg tctgcaccga tccggccgat ccggacggat
     5821 cgtgggtcac catcgacgac catgccgccg gacgcctggt gggccaacac atccgtcggc
     5881 tgggtcacca ccgggtggcc ctcgtggtcg agcaggcggt acccctgcgc gaggccgaac
     5941 cggcccagca gtccatggcc acctaccgcg ccgagctcga tgactaccgc acgctcggtc
     6001 tctacgacct gtgggggcgg gtcgaaggtt ttctcgccga actggacggg ctggacgtcc
     6061 aggtggtgag tgtcggactg aactcccgac agtccggacg agcggccgcc gaactgctgc
     6121 tcgaccagtt ggcgcgaccc accgcgatca tctgcgtcag cgacgtgatc gctttcggag
     6181 tactcgacgc catgcacgat cggggcctgg accccggccg cgacgtgacc gtgaccggtt
     6241 tcgacgacct gcccgaggcc gggcggctgg gcctgaccac cgtgcaccag ccgattcagg
     6301 aaaagggccg gctggtgggt gagttgctgc tcgatcccac ccgcaccccc cggcggatca
     6361 ccctgccgca ccaactgatc gtccgctcca gctcggcgcc accgcacggt agctgagcct
     6421 gcagtccggc tgagcccggc gtgcggctga atccacagtc cggctgagcc tcccgttccg
     6481 gctgagcctc ccgttccggc tgagcctccc gttccggccg agcccgcagt ccgggtccgg
     6541 ctgagcccgg ggtccggcag cccggctcag cccgggggcc ggctgagcct cacgtttccg
     6601 gctgacccgc agtgacccga tcgtccgttc ggcacagcac accgaccccc gggtcgctgt
     6661 gccgctgagc cggacctccc gtcgtcacac cgcactacac ccacctgatt acccacctgc
     6721 gtgagccatg cccacgcgac ctcgaaagga accacgatgt acctcgttct caactccacc
     6781 gatgccgccc gcgctctcaa cggcagcgca cggcccgacg cgcccgtcat cggcgaactg
     6841 gcctcaaagg cgcgccgcga cctgctcggt gacctcgccc tgcgcgtctc actggccggc
     6901 gagcgactca cccgccgcct gcggcgcaac ggccgggcgg gtcacgcggc cgccctgtcg
     6961 cactcctgac tcctgagcgg ctgagccgga ccacggccac gacgcccacg ggatgatcgt
     7021 cgaactggcc ctgaccgttc agaccttcgc gccggccatc ggccagcctc gccgagggcg
     7081 cgactcggcg cgtggcgcat ccggccgcac cggtcagcac cgccacacct gctctcaggc
     7141 tcccgctctg gcacgctaag cgcatggaag tgcttccgct ggcgttcacc ggcggctggg
     7201 cgagcggcat caacgcctgg gcgacggtgc tgatgctcgg cctgctcggc cggttcgccg
     7261 gcgtcgaggg cgtgccggcc ggcttcgaac gcaccgatgt gttgatcgtg atggcggtgc
     7321 tggcggtcgc cgaactggtc gcggacaaga ttccctacct cgattcggcc tgggacgcgg
     7381 tctcgacctt catccgtccg atcgccggag cgaccatcgg tgccctgatc gcgggccaca
     7441 ccggcgatct cggcaccatg gcattggctg cggtcggcgg cgtcaccgcg ctgctgtcgc
     7501 acctggccaa atccagcctg cggctggcga tcaacacctc gccggaaccc gtgaccaacc
     7561 tgaccgcctc agccgcgggc gacctctccc tggtgggtgt gctcgcactc gccgccctca
     7621 atccggtcgc ggcggccacc atcgccgccg cgctactggt gctcaccctc ggcctggccc
     7681 tgtttctgtt gaaccgggtg ggccggggtt ggcgggcctt ccgggtctgg ctggcccgcc
     7741 gaggcagggc atgacagtta tcgcggtcgt cggcggctca ccggcaggca tggcggcggc
     7801 cgcacggctg gccaaacaac ggcaccgggt gctgctcttc gaagcctccg atcgcctggg
     7861 cggacgctgg gcgcagcccg gtgccctgcc cccggtgctg agccttcccg cgccatggcg
     7921 cgacctcttt cgcaaatccg gacgcgcctt cgacgtcgag ttggcccgca ccgggctcgc
     7981 cctggtcgcc gccgcccccg ccgtccaccg gttcgcggac ggctcggagt tgaccctgcc
     8041 caccgaccgt ggcgagcagt ggacgacgct gaccgcagcc tggggcaccc cggcggcgac
     8101 ccgctggcgc gacctgctcg acgacctcga tcagcgttgg cagctgctgc gccgactagg
     8161 cctggaggac gagttcaccg aagtggcgct caccccggcc cgacgccggg cactcgggct
     8221 cgaccgcacc gtcgaaaaac tggcccgaga cttcgggcat ccccaggccg cggcgctgat
     8281 tcgctccacc gcctggcgct tcggatcgaa accggcccgc acccccgcgt tcgtggccgt
     8341 gcggctggcg atcgaccgca cgttcggacg ctggcaactc acccgcgacg gccatccggt
     8401 gccggcgtcg gccctgatcg atctgctcgc cgaccggctg accctgcgcg gcgtcgaggt
     8461 gcattcgggc cgaccggtca cggcgctcgc gcccggccgg gtgcgtaccg ccgacggcga
     8521 catcgtggtc gacgcgatcg tggccgcagt gaatccgtgg acctacctga ggctcaccgg
     8581 cacccaggaa cgcgtgctga gccgccgaac cgcctggacg cgccccgccc ttgccccccg
     8641 gatcaccgtt gaccaagaag acagtgtgag ttcaggcctt gagtaccagg cccactctca
     8701 cactgtcatt cacgagattc gtgaaacggt gcagcactcc gctgtcggag ggtgggtcga
     8761 ctaccggctg cccagcccaa cgggcatcct caccgtgcgc cacgacttca gcgatcccgt
     8821 gcccgatccc ggcttcggca cccgctggac ctcgccccgc acctggcttc ggcaaccgcc
     8881 gctgcgggca gcgaccccga cggtggtcac cgcgagcgcg tccagccggg gcggcaacga
     8941 cccctgggcg caactgctgt cgggtgcgtt ggccgcgtat gtggtgcatg agaacctgac
     9001 cggagccgac atccgtccga ccaacaaggc ggcgcgaccc tagctggtta ctcgtcattc
     9061 cgggcctgcc ccgggattcc ggcgtgcgcc gggatgacag ccgtgacgac ggagaacgct
     9121 gatcattgcg caatccgatg gttcagcttg atcagcgttt ccatcgcgtg acccggcgcc
     9181 tgcgctacag gatgatcagc accagcagca ggatcgccac cgcgatgccg gtgcccaggc
     9241 cggcgaccag catcagcgcg cccttgttgc cgggaccctt cgcgggcacc agttcaccgg
     9301 gtgcaaccgt cccggtctcg atcaccgcgg gcaccgcaaa ggtgtctcta cccggcaatg
     9361 ctgccgctac ctcggccacc tggcccagcg tctgcgacgc gaacacctgg tcgagcatcg
     9421 cccggtaggt atcggtgtcc atgccgccct tggcgtactc agcgttgagc cgttcggcaa
     9481 gctgctcacg ctcggcatcg tccagcggat cgagcgggcg ggagcggtac ttcgacgaga
     9541 tcggcaggtt gtccacccac ccaggttagt tggggcaggc ggggtctcga cacgctcgac
     9601 caacgcaggc cgagccggtt gccctcggag cccgcgaccg cctgcgtgaa ggcagcaacc
     9661 gggtcggccg cggctgaccc gcgatctcga caccgccgag cccgaggggt cggcgtcggc
     9721 tcagcccact accgtcatct cgacctcgac atacatctcg gacgctccgc cgccagaata
     9781 gatgccccgc agcggcggca cgtcggagta gtcgcgtccg atgc
//
LOCUS       DAOSUG010000037         9646 bp    DNA     linear   ENV 10-JUN-2024
DEFINITION  MAG TPA_asm: Micropruina sp. isolate AMR_MDS_2195
            NODE_3844_length_9646_cov_3.785841, whole genome shotgun sequence.
ACCESSION   DAOSUG010000037 DAOSUG010000000
VERSION     DAOSUG010000037.1
DBLINK      BioProject: PRJNA925477
            BioSample: SAMN32864262
            Sequence Read Archive: SRR4236673
KEYWORDS    WGS; ENV; Metagenome Assembled Genome; MAG; Third Party Data; TPA;
            TPA:assembly.
SOURCE      Micropruina sp. (wastewater metagenome)
  ORGANISM  Micropruina sp.
            Bacteria; Bacillati; Actinomycetota; Actinomycetes;
            Propionibacteriales; Nocardioidaceae; Micropruina.
REFERENCE   1  (bases 1 to 9646)
  AUTHORS   Abdulkadir,N., Saraiva,J.P., Zhang,J., Stolte,S., Gillor,O.,
            Harms,H. and Rocha,U.
  TITLE     Genome-centric analyses of 165 metagenomes show that mobile genetic
            elements are crucial for the transmission of antimicrobial
            resistance genes to pathogens in activated sludge and wastewater
  JOURNAL   Microbiol Spectr 12 (3), e0291823 (2024)
   PUBMED   38289113
REFERENCE   2  (bases 1 to 9646)
  AUTHORS   Abdulkadir,N.
  TITLE     Direct Submission
  JOURNAL   Submitted (24-MAR-2023) Environmental Microbiology, Helmholtz
            Center for Environmental Research -UFZ, Permoserstr 15, Leipzig
            04318, Germany
COMMENT     The annotation was added by the NCBI Prokaryotic Genome Annotation
            Pipeline (PGAP). Information about PGAP can be found here:
            https://www.ncbi.nlm.nih.gov/genome/annotation_prok/
            
            ##Genome-Assembly-Data-START##
            Assembly Method        :: SPAdes v. 3.11.1
            Genome Representation  :: Full
            Expected Final Version :: Yes
            Genome Coverage        :: Not Applicable
            Sequencing Technology  :: Illumina MiSeq
            ##Genome-Assembly-Data-END##
            
            ##Genome-Annotation-Data-START##
            Annotation Provider               :: NCBI
            Annotation Date                   :: 05/04/2023 04:57:10
            Annotation Pipeline               :: NCBI Prokaryotic Genome
                                                 Annotation Pipeline (PGAP)
            Annotation Method                 :: Best-placed reference protein
                                                 set; GeneMarkS-2+
            Annotation Software revision      :: 6.5
            Features Annotated                :: Gene; CDS; rRNA; tRNA; ncRNA
            Genes (total)                     :: 2,776
            CDSs (total)                      :: 2,750
            Genes (coding)                    :: 2,714
            CDSs (with protein)               :: 2,714
            Genes (RNA)                       :: 26
            tRNAs                             :: 24
            ncRNAs                            :: 2
            Pseudo Genes (total)              :: 36
            CDSs (without protein)            :: 36
            Pseudo Genes (ambiguous residues) :: 0 of 36
            Pseudo Genes (frameshifted)       :: 2 of 36
            Pseudo Genes (incomplete)         :: 32 of 36
            Pseudo Genes (internal stop)      :: 2 of 36
            ##Genome-Annotation-Data-END##
FEATURES             Location/Qualifiers
     source          1..9646
                     /organism="Micropruina sp."
                     /mol_type="genomic DNA"
                     /submitter_seqid="NODE_3844_length_9646_cov_3.785841"
                     /isolate="AMR_MDS_2195"
                     /isolation_source="Activated sludge of municipal
                     wastewater treatment plants"
                     /db_xref="taxon:2737536"
                     /environmental_sample
                     /geo_loc_name="Canada"
                     /lat_lon="49.25 N 123.1 W"
                     /collection_date="2015-06-16"
                     /metagenome_source="wastewater metagenome"
                     /note="metagenomic"
     gene            <1..369
                     /locus_tag="PLF56_02230"
     CDS             <1..369
                     /locus_tag="PLF56_02230"
                     /EC_number="2.7.1.121"
                     /inference="COORDINATES: protein motif:HMM:NF014753.2"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_function: GO:0004371 - glycerone kinase activity
                     [Evidence IEA];
                     GO_process: GO:0006071 - glycerol metabolic process
                     [Evidence IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="dihydroxyacetone kinase subunit DhaK"
                     /protein_id="HOQ52411.1"
                     /translation="DMEVGMGIHGEPGIERKKIEPADAVVDEILGIVLPEMNLASGDE
                     VAVMINGLGGLPLMDQYICYRRVHDVLTEKGVTIAKSMVGNYATSMDMIGMGVTLVKL
                     DDELKGWLNAPFNAPYLKQA"
     gene            374..1012
                     /gene="dhaL"
                     /locus_tag="PLF56_02235"
     CDS             374..1012
                     /gene="dhaL"
                     /locus_tag="PLF56_02235"
                     /inference="COORDINATES: protein motif:HMM:TIGR02365.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_function: GO:0004371 - glycerone kinase activity
                     [Evidence IEA];
                     GO_process: GO:0006071 - glycerol metabolic process
                     [Evidence IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="dihydroxyacetone kinase subunit DhaL"
                     /protein_id="HOQ52412.1"
                     /translation="MADFDRDYFVRVFRAMAVAAEEHRDEWTKLDGAIGDGDHGINMS
                     IGFREVVKQLDALPDDADIAGTFKLVGMTLLRKVGGASGPLYGSFFMKAGAEVADQSE
                     VSLDDLRRMLRAGVASIQARGKAELGDKTMLDSYLPGLDVLDAADVADPKAALTAFVE
                     RMRQGSDDTIPLQAKKGRAMRLGPRSIGHRDPGSQSSWMLMQCFVDELAPVG"
     gene            complement(1277..1939)
                     /locus_tag="PLF56_02240"
     CDS             complement(1277..1939)
                     /locus_tag="PLF56_02240"
                     /inference="COORDINATES: ab initio
                     prediction:GeneMarkS-2+"
                     /note="Derived by automated computational analysis using
                     gene prediction method: GeneMarkS-2+."
                     /codon_start=1
                     /transl_table=11
                     /product="hypothetical protein"
                     /protein_id="HOQ52413.1"
                     /translation="MKRLLPLLLVGLLSACTVGQPGASTPPATPEPSISTPATDEASP
                     SGSAGATETTSPSGTASDSPSASPSVTDSSTGSASPTATTTGSAGSFRAPGEVLDLNG
                     EVTVTALDSKRAKVAGHRGNQVLVMVKSCNVSAAQGIELTWDTWVLLGPDSEEYPPSE
                     VKGSNEPAPIYPNASGRLYKPGQCAKGWVVFDVPNGAALSGVRYMNSQGDSAQWRLSA
                     SV"
     gene            complement(2059..2892)
                     /locus_tag="PLF56_02245"
     CDS             complement(2059..2892)
                     /locus_tag="PLF56_02245"
                     /EC_number="3.-.-.-"
                     /inference="COORDINATES: protein motif:HMM:NF013672.2"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_function: GO:0016787 - hydrolase activity [Evidence
                     IEA];
                     GO_function: GO:0046872 - metal ion binding [Evidence
                     IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="polysaccharide deacetylase family protein"
                     /protein_id="HOQ52414.1"
                     /translation="MAAIVVAGIRANAGERSAPPSGSRQSSSVTGGKVKKTPTTTTPQ
                     QNKSDGASATKQAGSTTKPTGTTKTSGTKTRLHSGYYYRTGPNRSARVVLSYDDCPRS
                     LAEFKTVIVGAQKLGIGLMLFPTGVCLQSGQFSTSFARAHGHYVFNHSISHPDLARLS
                     SAGVLHQLGAPGVQSRYGRPPYGSYSPTVAKAYAAKGMKIWTWTVDTLDWRGRSAQSV
                     ISTAVHGPKAGGTVLMHMQWHGFSVAALEQIQAGLAERGLDVCRNFPGTTPADSWTVR
                     C"
     gene            complement(3042..4010)
                     /locus_tag="PLF56_02250"
     CDS             complement(3042..4010)
                     /locus_tag="PLF56_02250"
                     /inference="COORDINATES: protein motif:HMM:NF013088.2"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_component: GO:0016020 - membrane [Evidence IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="EamA family transporter"
                     /protein_id="HOQ52415.1"
                     /translation="MPARHRLLAALVAVIWGLNFLAIHASLAQFPPFFLVALRFALIA
                     VPTVLFVPRPRVRLRWLIGYGLGFGVAQFTFLYWGMAAGMPTGLASLVLQASAPFTLA
                     LGAVLLRERVRPAQWAGIAIAVAGMLLVGASRAQASGFWPFVLVLAGGLGWAFGNLAS
                     RQAKPDNPLHLTLWMSVVVPVPMLALSLLVEGPQQIATALTTSFSSAALPAWAGLAYT
                     CVIGTAVGSGIWTWLLARHPAGVVAPFSLLVPVVGLLTAAVVLGEIPTSLDLIGALVV
                     VAGVLIGARSPRGTARPGSLTATDPTRVRTPSLIDDPDGRQPSTAT"
     gene            4083..4979
                     /locus_tag="PLF56_02255"
     CDS             4083..4979
                     /locus_tag="PLF56_02255"
                     /inference="COORDINATES: protein motif:HMM:NF015431.2"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_function: GO:0003700 - DNA-binding transcription factor
                     activity [Evidence IEA];
                     GO_process: GO:0006355 - regulation of DNA-templated
                     transcription [Evidence IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="LysR family transcriptional regulator"
                     /protein_id="HOQ52416.1"
                     /translation="MDLRHLELLRELADRGTITAVAEATFRSPSAVSQQLQTAERAFG
                     VALVEPDGRRLRLTAAGRLLALGAADVATTLARLQHEVDALQGEPSGLVRVAALPSAA
                     EFLLPPVLRSLAGGAVTLTLTDDDVAEADFGRRTADHDVVIGHTLAEVPAGTERLSVL
                     TLAREPLEVAVPADHRLAGRTHVRASDLAGERWIGVPLGYPFDTVRIAIDNRSGQPLQ
                     VVQRIRDNRVVESLVASGEGCALLPRFTTLGRPGVVTLPLADVRSVRTIVALGRPDRW
                     QRAAVRLVLDALAQVGATMGDR"
     gene            complement(5690..6157)
                     /locus_tag="PLF56_02260"
     CDS             complement(5690..6157)
                     /locus_tag="PLF56_02260"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_013160864.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="GatB/YqeY domain-containing protein"
                     /protein_id="HOQ52417.1"
                     /translation="MAAIKDRLKTDLVAALKAHDETRKSTVRMALAAIANEEVAGKTA
                     RELSDAEEKAVLAKEVSKRKDSAEAYTAGNRPELAEKELAEAAILADYLPQQLSEAEI
                     AAIVDEEVAAVTASLGEKPTMRQMGLVMKAVTPRIAGRGDGKTAAGLVRAALG"
     gene            6271..6837
                     /gene="thpR"
                     /locus_tag="PLF56_02265"
     CDS             6271..6837
                     /gene="thpR"
                     /locus_tag="PLF56_02265"
                     /inference="COORDINATES: protein motif:HMM:TIGR02258.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_function: GO:0008664 - 2'-5'-RNA ligase activity
                     [Evidence IEA];
                     GO_process: GO:0008033 - tRNA processing [Evidence IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="RNA 2',3'-cyclic phosphodiesterase"
                     /protein_id="HOQ52418.1"
                     /translation="MTLRLFAAVFPPADVLDTLDLFVEPRRVADDNLRWVRRDNWHLT
                     LAFFGDVHDDHLEPLQHALDEVTGTPFTLTLDGAGTFPNPSQARVLYRATTTGSDALS
                     ALSRRVRTAAERVGVPSDNAKHQPHLTLARTRRPAPLTRWLGIVDSFPPQAFTVDSFA
                     LIRSDLHPSGPAYRLLHTVDLAGRPDCD"
     gene            6902..8182
                     /locus_tag="PLF56_02270"
     CDS             6902..8182
                     /locus_tag="PLF56_02270"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_011757060.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="O-acetylhomoserine
                     aminocarboxypropyltransferase/cysteine synthase"
                     /protein_id="HOQ52419.1"
                     /translation="MTYQPETLAVHAGQEDADPATNARAVPIYQTTSYVFDDTQHAAD
                     LFALKVPGNIYTRIMNPTQNVFEARMTALEGGVGALATSSGAAAITYAVLNLTYTGDN
                     IVALSTLYGGTYALFAHTLPQFGIEVRFVDPEKPDELAQHVDEKTKLVFGETVGNPLV
                     NVVDLDVWSAAAHALGLPFIVDNTVPTPYLCRVFEHGVDVAVHSATKYIGGHGTSIGG
                     VIVDSGNFDWTAHAERFPGLTSPDHAYHEVVWTEAVGNLAYIIRARTVLLRNTGAAVT
                     PMNSWLFLQGLETLHLRMERHSSNALAVAQYLEQHPAVAWVNYPGLESSQWHEIGRRI
                     LPRGAAGIVSFGLKAGREGGSKLVESVQLFSHLANIGDAKSLIIHNASTTHSQLTEAE
                     LETAGVAPEMVRLSVGIENVNDLIADLDQALAQF"
     gene            8187..9326
                     /locus_tag="PLF56_02275"
     CDS             8187..9326
                     /locus_tag="PLF56_02275"
                     /EC_number="2.3.1.31"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_014846613.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_function: GO:0016747 - acyltransferase activity,
                     transferring groups other than amino-acyl groups [Evidence
                     IEA];
                     GO_process: GO:0009058 - biosynthetic process [Evidence
                     IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="homoserine O-acetyltransferase"
                     /protein_id="HOQ52420.1"
                     /translation="MTAPGSVGLVQTQTARLFTPDAPLRLANGSTLGTVDVAYETYGT
                     LSAAKDNAVFICHALTGDAHAAGFHEGAKRPGWWDNLIGPGKPLDTDRFFVISANLLG
                     GCQGTTGPSSIDPATGRAHGLEFPLLDMADFVTVHRALLAHLGIERLLAALGGSLGGM
                     QVLDWSLRHPEQVTNAAIVAASSRLTAQNIAFSAVARRAIMDDPDFHDGRYAERGVVP
                     RLGLRTARMMAHITYLSEDAFSEKFGRTPHPERDKPGFGIDFAVESYLDHQGKVFLER
                     FDALSYLYLSRVMDYFDPFSRPDPVQRIMADPVNFLVLSFNTDWRFSTEHSRRIVRHL
                     ENARQPVTFRDIPSSWGHDSFLLPVARYHDTLRGWFGRALREGLR"
     gene            9323..>9646
                     /locus_tag="PLF56_02280"
     CDS             9323..>9646
                     /locus_tag="PLF56_02280"
                     /inference="COORDINATES: protein motif:HMM:NF018696.2"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="methionine biosynthesis protein MetW"
                     /protein_id="HOQ52421.1"
                     /translation="MTLRPDLNLIAHLVPGSSRVLDLGCGDGTLLHHLISDHDCTGTG
                     VEIDPDKVLGAIDAGVPVMELDLDADLALFDTGSFDTVVLSRTIQTLRHPELVLLGMA
                     RIGRQL"
BASE COUNT         1526 a         3306 c         3282 g         1532 t
ORIGIN      
        1 gacatggagg tcggcatggg cattcacggc gagcccggca tcgagcgcaa gaagatcgag
       61 cccgccgacg cggtcgtgga cgagattctc ggcatcgtgc tgcccgagat gaacctggcc
      121 tccggcgacg aggtggcggt gatgatcaac ggtctgggcg ggctgccgct gatggaccag
      181 tacatctgct accgccgggt gcacgacgtg ctgaccgaga agggcgtcac catcgccaag
      241 tcgatggtcg gcaactacgc caccagcatg gacatgatcg gcatgggcgt cacgctggtg
      301 aagctcgacg acgagctgaa gggctggctg aacgcaccct tcaatgctcc ctacctgaag
      361 caggcctgat ccgatggctg acttcgaccg cgactacttc gtgcgcgtgt tccgtgccat
      421 ggcggtcgcc gctgaagagc atcgcgacga gtggacgaag ctggacggcg ccattggcga
      481 cggcgaccac ggcatcaaca tgagcatcgg ttttcgcgag gtggtcaagc aactggacgc
      541 cctgcccgac gacgccgaca tcgccggcac gttcaagctg gtgggcatga cgctgttgcg
      601 caaagtgggc ggcgcgtccg gcccgctgta cggcagcttc ttcatgaagg ccggggccga
      661 ggtggccgat cagagcgagg tcagcctgga cgacctgcgg cgcatgctcc gcgccggggt
      721 cgcgtccatc caggcgcgcg gcaaggccga actgggcgac aagacgatgc tcgacagcta
      781 cctgcccggg ttggacgtgc tcgacgcggc ggacgtcgct gacccgaagg cggccctcac
      841 cgccttcgtc gagcgcatgc gccaaggctc ggacgacacc attccgctgc aggccaagaa
      901 ggggcgcgca atgcggctcg ggccacgttc gatcggccat cgcgacccgg gctcccagtc
      961 gtcctggatg ctgatgcagt gtttcgtgga cgaactggcg ccggtcggct gaacgctcgg
     1021 gctcggagtg ttcgtccgtt gggcgagctt gtcgaggcct ggctgaatgc cgttggtcga
     1081 gcgtgttgag atgcggctga atcgcgtcgt cggttgaggt tggtgttggg cctggctgag
     1141 cggtctcgac acgctcgacc acctcgatcg ctggtcaaag accgacctga ggagttcgac
     1201 tcgcacccca gcgggatcgc tggtcgagac ccgccggacg agagtcgacc cccaccccaa
     1261 cgggagctag acggagctag acgctggcgc tgagccgcca ctgtgccgag tcgccctggc
     1321 tgttcatgta acgcacgccc gacagggcgg ccccgttcgg tacgtcgaac accacccagc
     1381 ccttggcgca ctggccaggc ttgtagagcc gtccgctggc gttggggtag atcggagcgg
     1441 gctcgttcga gcctttgacc tcgctgggtg ggtactcctc gctgtcgggg ccgagcagca
     1501 cccaggtgtc ccaggtcagt tcgatgccct gcgcggccga gacgttgcag ctcttcacca
     1561 tcaccagcac ctgattgccg cggtggccgg ccaccttggc gcgcttgctg tcgagtgcgg
     1621 tgacggtgac ctcgccgttc aggtcgagca cctcacccgg ggcccggaag ctgccggccg
     1681 agccggtggt ggtcgcggtg ggggaggccg aaccggtgct ggagtcggtg accgagggcg
     1741 aggcgctcgg gctgtcgctc gcggttccgg acggactggt cgtctccgtc gcgccggcac
     1801 tgcccgacgg ggacgcctcg tcggtggccg gcgtgctgat ggacggctcg ggcgtggcag
     1861 gcggcgtgga cgcacctggc tgcccgacgg tgcatgcgct caacaggccg accagcagga
     1921 ggggtagcag acgcttcatg ggcagacact acggcgggcg atgtcctggc gataccccgt
     1981 agcggctgac gtcacggacc agcagcgagg tgcagcgaag gctgagctca ggcccggtga
     2041 gcgtggatca gtgttgcctc agcagcgcac ggtccacgag tccgccggcg tggtgccggg
     2101 aaagttgcgg cacacatcga gaccgcgctc agccaaaccg gcctggatct gctcaagggc
     2161 cgcgaccgag aagccgtgcc actgcatgtg catcaggacg gtgcccccgg ccttcgggcc
     2221 atggacggcg gtcgagatca cgctctgcgc ggaccgacca cgccagtcga gcgtgtcgac
     2281 ggtccaggtc cagatcttca tgcccttggc cgcgtaggcc ttcgccacgg tggggctgta
     2341 cgagccgtac ggcgggcgtc cgtagcggga ttgaaccccc ggcgctccga gctgatgcag
     2401 cacgcccgcg gacgacaacc gagccagatc ggggtggctg atcgagtggt tgaacacata
     2461 atgcccgtgg gcccgcgcga acgaggtcga gaactgaccg ctctgcaggc acacgccggt
     2521 cgggaagagc atcagcccga tgcccagctt ctgggcgccg acgatcaccg tcttgaactc
     2581 ggcgagactg cgcgggcagt cgtcgtagct cagcacgacc cgtgccgaac gattgggccc
     2641 ggtgcggtag tagtagccgc tgtgcagacg ggtcttggtg cccgatgtct tggtcgtgcc
     2701 ggtcggcttc gtggtggacc cggcctgctt ggttgctgac gccccgtcgg acttgttctg
     2761 ttggggggtc gtcgtggtgg gagtcttctt caccttgccc ccagtgaccg acgacgattg
     2821 cctggagccc gacggtgggg ccgaacgctc gcctgcattg gcacgaatac cggccacgac
     2881 aatggctgcc acgaccacaa ccgtgatggt ggtcattagc agacgtccca aacgcacggc
     2941 tcaatctccc ccggggtgac gtgggcggtt atcggcccac gccctcagtc taggtgggcg
     3001 cgcgaacgcc cctggtcagg ccggcggcga cccgccgacc ctcaagtcgc ggtcgatggt
     3061 tgccggccgt ccggatcgtc gatgagcgac ggggtgcgga cgcgggtcgg gtcggtggcg
     3121 gtgagactcc ccggccgggc ggtgccgcgc ggactccgcg ctccgatcag cacgccggcg
     3181 accacgacca gggcgccgat caggtcgagc gaggtgggga tctcgcccag tacgacggca
     3241 gcggtgagca ggcccaccac cggcaccagc agcgagaacg gggccaccac gccggcggga
     3301 tgccgggcca gcagccacgt ccagatcccg gagccgaccg ccgtgccgat cacacaggtg
     3361 tacgccagac cggcccaggc cggcagggcg gcgctactga acgaagtggt cagtgcagtg
     3421 gcgatctgct gcggaccctc gaccagcagc gacagcgcca gcatcggcac cggtaccacc
     3481 accgacatcc acagggtcag gtgcaatggg ttgtcgggct tggcctgccg gctggccagg
     3541 ttgccgaagg cccagcccag tccgccggcg agcaccagca cgaacggcca gaagccggac
     3601 gcctgggccc gagaggcacc caccagaagc attccggcaa cggcgatcgc gatgccggcc
     3661 cactgcgccg gccgcacccg ttcacgcagc aaaacggcac ccagtgcaag cgtgaacggc
     3721 gcggacgcct gcagcaccag cgaagccagg ccggtcggca tgccggcggc catgccccag
     3781 tagagaaacg tgaactgcgc cacgccgaag ccgagtccgt agccgatcag ccagcgaaga
     3841 cgaacccgcg ggcggggcac gaacagcacc gtcggcacgg cgatcagggc gaaccgcagt
     3901 gccaccagaa agaacggcgg aaactgcgcg agcgaggcgt gaatcgccag gaagttcagg
     3961 ccccagatga cggcgaccag ggcggcgagc agacgatgac gagcgggcac acgtgcaccc
     4021 tcgcgcagaa gaaaggtgaa gaacaagcgc attgttctgc acaactgatt taggctgcct
     4081 tcatggacct gcgccatctg gagctgctgc gcgaactggc cgaccggggg acgatcaccg
     4141 ccgtcgccga ggcaaccttc cgctcgccct cagcggtgag ccaacaactg cagacggccg
     4201 agcgtgcctt cggcgttgcc ctggtcgaac cggacggacg ccggctgcgc ctcaccgccg
     4261 ccggccgatt gctggcgctc ggtgcggcgg acgtggccac cacgctggcc cggctgcagc
     4321 acgaggtgga cgccctgcag ggcgagccca gcggcctcgt ccgggtggcc gcattgccga
     4381 gtgcggcgga gtttctgctg ccgccggtgc tgcgctcgct ggctggtggc gcggtcaccc
     4441 tcacgctcac cgatgacgat gttgccgagg ccgacttcgg ccggcgaacc gccgatcacg
     4501 atgtggtgat cgggcacacg ctggcggagg taccggccgg cacggagcgg ctgagcgtgc
     4561 tgacgttggc gcgcgagccg cttgaggtgg ccgtgccggc cgatcaccga ctggcgggcc
     4621 gcacccacgt gcgggcgtcc gatctggcgg gggagcgctg gatcggggtg ccgctggggt
     4681 acccgttcga cacggtgcgc atcgcgatcg acaaccgcag cggccagccc ctgcaggtcg
     4741 tgcagcggat ccgtgacaac cgggtggtcg agtccctggt cgcgagcggc gaaggttgcg
     4801 cgctgctgcc gcgcttcacc acccttggac gtccaggggt ggtgactctg ccgctggccg
     4861 acgtgcgttc ggtgcgcacg atcgtggccc tgggccgtcc ggaccgctgg caacgggcgg
     4921 ccgtccggct ggtgctggac gccttggcgc aggtcggcgc gacgatgggc gaccgctgat
     4981 cgagcctgtc gagtcccttc ccgtcacccc ttccccgtca ccccggcgcc tctcccgtca
     5041 tgccggcccc cttccctgtc atgccggccc ccttcctcgt catcccggcg aatgccggga
     5101 tctctccgcg tccccaatgg ttagtgagga cactcctggt ctggggtttt cgagaactgc
     5161 gctggtcgag ctctgagacc tggagccccg gtggtcggga ccacacccac aaccgccttg
     5221 tggatgtaga tgggggcagt tctttcctga agacgtcgcg accctgccaa cgtgactgcc
     5281 cttgtcacga cggcagttgt gtcggggtgg ggtgtcgtga gggcagttgt gtctgggtgg
     5341 ggtgtcgtga gggcagctac gtccgagtgg ggtgtcacga gggagcttcc cagtgcgctg
     5401 cacggagttg ggaaccgtcc ccactccgtt cacccgctag gcagggacga tcggagccgg
     5461 aattgcggca gcgtccacga gcggaagtcg gaggtggggg aggggggccc gacccgagtc
     5521 gtggatgggt ctggccgccg agggagcttg cgaccgaggc tggttggctc gggtcgggcc
     5581 cccctccgcc accgacctca tcgctcgact cgcacgggcg atggtctcga cgaaatcgac
     5641 cagcggcctc gggcaagctc gaccggcaac ggtcccgacg cggcggcgcc taccccaggg
     5701 cggcgcgcac cagaccggcg gcggtcttgc cgtcaccgcg tccggcgatg cggggggtca
     5761 ccgccttcat caccaaaccc atctgccgca tggtcggctt ctcgcccagg gaggccgtca
     5821 cggccgccac ctcttcgtcg acgatcgcgg cgatctcggc ctcgctgagc tgctggggca
     5881 ggtaatccgc caggatcgcc gcctcggcca gttccttctc ggccaactcc ggacgattgc
     5941 ccgcggtgta cgcctcggcc gaatccttgc gcttggacac ctcttttgcc agcactgcct
     6001 tctcctcggc gtcgctgagc tcgcgcgcgg tcttgccggc gacctcttcg ttggcgatgg
     6061 cggccagggc catgcgcacg gtcgacttgc gtgtctcgtc gtgggccttg agcgccgcca
     6121 ccaggtcggt cttgaggcgg tccttgatcg ctgccatgtg ctgacgtcct ttcgggtatc
     6181 cagctcattg tcgcacccgc gtgctgtcac ccgacgcgcg acggctttgg ccaccgatca
     6241 tcgaccgatc cgtgccagac tggggcaccg atgacattgc gcctcttcgc cgccgttttt
     6301 cctcccgccg acgttctcga caccctggat ctcttcgtcg aaccacgccg agtcgccgac
     6361 gacaacctgc gctgggtgcg gcgcgacaac tggcacctca cgctggcctt cttcggtgat
     6421 gtgcacgacg accacctcga gccgctgcag cacgctctgg acgaggtcac cggtacgccc
     6481 ttcactctca ccctggacgg cgccggcacc tttccgaacc cgtcccaggc ccgcgtgctc
     6541 taccgtgcca ccaccaccgg ctcggacgca ctgagcgcgc tgagccgccg cgtccggacc
     6601 gccgccgagc gtgtgggagt gccgtccgac aacgccaaac atcaacccca cctgaccctg
     6661 gcccgtactc gtcggccggc cccgctgacc cgctggttgg gcatcgtgga cagctttccg
     6721 ccccaagcct tcaccgtcga ctcgttcgcg ttgatccggt ccgacctgca ccccagtggc
     6781 cccgcctacc ggttgctgca caccgtcgac ctggccggac gtccggactg cgactgacca
     6841 gcggtcaagt cgcggcacac cattaggctg accgggccgt ccgacgaagg tgagcgaacc
     6901 catgacctac cagcccgaaa ccctggccgt ccacgccggg caggaggacg ccgacccggc
     6961 gaccaacgcc cgcgcggtgc cgatctacca gaccacgtcg tacgtgttcg acgacaccca
     7021 gcacgccgcc gacctgttcg cgctcaaggt gccgggcaat atctacaccc gcatcatgaa
     7081 ccccacccag aacgtgttcg aggcgcggat gacggctctc gaagggggtg tgggtgcgct
     7141 ggcgaccagt tcgggggcgg ccgcgatcac ctacgcggtg ctcaacctga cctacaccgg
     7201 cgacaacatc gtggccctct ccacgctcta cggcggcacg tacgcgctgt tcgcccacac
     7261 cctgccgcag ttcggcatcg aggtgcgttt cgtcgatccc gagaagccgg acgaactcgc
     7321 ccagcacgtc gacgagaaaa ccaagctggt cttcggcgag accgtcggca acccgctggt
     7381 caacgtggtc gacctggacg tctggtcggc cgccgcgcac gcgctgggcc tgccgttcat
     7441 cgtcgacaac acagtgccga ccccgtacct gtgccgggtg ttcgagcacg gcgtcgacgt
     7501 cgcggtgcat tcggccacca agtacatcgg cggccacggc acgtccatcg gcggcgtgat
     7561 cgtcgactcc ggcaacttcg actggacggc gcacgccgag cgcttccccg gcctgaccag
     7621 ccccgaccac gcctaccacg aggtggtctg gaccgaggcc gtcggcaacc tcgcctacat
     7681 catccgcgcc cgcacggtgc tgctgcgcaa caccggcgcc gcggtgacgc cgatgaacag
     7741 ctggctgttc ctacaagggc tggagacctt gcacctgcgc atggagcggc acagcagcaa
     7801 cgcgctggcg gtggcgcagt accttgagca gcacccggcg gtggcgtggg tcaactaccc
     7861 ggggctggaa tcgtcccagt ggcacgagat cggccggcgc attctgccca ggggggcggc
     7921 gggcatcgtc tcgttcggcc tgaaggccgg ccgcgaaggc ggttcgaagc tggtcgagtc
     7981 ggtgcaactg ttcagccacc tggccaacat cggcgacgcg aagtcgctga tcatccacaa
     8041 cgcgtccacc acgcacagcc agctcaccga agccgaactc gagaccgccg gcgtcgcgcc
     8101 cgagatggtg cgactgagcg tgggcatcga gaacgtgaac gacctgatcg ccgacctgga
     8161 tcaggcgttg gcgcagttct gaaccgatga cagcacctgg ttccgtggga ctggttcaga
     8221 cccagaccgc tcggctgttc accccggacg ccccccttcg gctggccaac gggtcgacgt
     8281 tggggactgt cgacgtcgcc tatgaaacct acggcacgct gtccgccgca aaggacaacg
     8341 cggtgttcat ctgccacgcc ctcaccggcg acgcccacgc ggcgggcttt cacgagggcg
     8401 cgaagcgtcc gggctggtgg gacaacctga tcggcccggg caaaccgctc gacaccgaca
     8461 ggttcttcgt gatcagcgcc aacctgctgg gcggctgcca gggcaccacc ggaccgtcct
     8521 cgatcgaccc cgccaccgga cgggcccacg gcctggagtt tccgctgctc gacatggccg
     8581 acttcgtcac cgtgcaccgg gcgctgctgg cgcacctggg catcgagcgg ctgctcgccg
     8641 cgctcggggg ctcgctggga gggatgcagg tgctggactg gtcgctgcgc caccccgaac
     8701 aggtcacgaa cgcggcgatc gtcgcggcct catcgagact gaccgcgcag aacatcgcct
     8761 tctcggcggt ggcgcggcgg gcgatcatgg acgaccccga ctttcacgac ggacgctacg
     8821 ccgagcgcgg cgtggtgccg cggctggggc tgcgtaccgc ccgcatgatg gcgcacatca
     8881 cctacctcag cgaggacgcg ttcagtgaga agttcggacg caccccgcac ccggagcgcg
     8941 acaagcccgg cttcggcatc gacttcgccg tcgagagcta cctcgatcac cagggcaagg
     9001 tgtttctcga acgcttcgat gcgctgtcgt acctgtacct gtcacgcgtg atggactact
     9061 tcgacccgtt cagccgcccg gacccggtgc agcgcatcat ggccgacccg gtgaactttc
     9121 tggtgctgtc gttcaacacc gactggcggt tcagcaccga gcattcgcgg cgcatcgtcc
     9181 ggcatctcga gaatgcgcgg cagccggtga cgttccgcga cattccctcc agttggggtc
     9241 acgactcgtt tctgctgccg gtggcccggt accacgacac gttgcgcggc tggttcggcc
     9301 gggcgttgcg ggaggggttg cgatgacgct tcgtcccgac ctgaacctga tcgcccatct
     9361 ggtgcccgga tcgtcgcggg tgctcgacct gggctgcggc gacggcaccc tgctgcatca
     9421 cctgatctcg gatcacgact gcaccggcac cggggtcgag atcgatcccg acaaggtgct
     9481 gggtgcgatc gacgcaggtg tgccggtgat ggaactcgac ctggacgccg acctggccct
     9541 gttcgacacc ggctcgttcg acacggtggt gctctcgcgg accatccaga ccctgcggca
     9601 ccccgagttg gtgttgctcg gcatggcccg gatcggtcgt cagctg
//
LOCUS       DAOSUG010000038         9613 bp    DNA     linear   ENV 10-JUN-2024
DEFINITION  MAG TPA_asm: Micropruina sp. isolate AMR_MDS_2195
            NODE_3858_length_9613_cov_4.055974, whole genome shotgun sequence.
ACCESSION   DAOSUG010000038 DAOSUG010000000
VERSION     DAOSUG010000038.1
DBLINK      BioProject: PRJNA925477
            BioSample: SAMN32864262
            Sequence Read Archive: SRR4236673
KEYWORDS    WGS; ENV; Metagenome Assembled Genome; MAG; Third Party Data; TPA;
            TPA:assembly.
SOURCE      Micropruina sp. (wastewater metagenome)
  ORGANISM  Micropruina sp.
            Bacteria; Bacillati; Actinomycetota; Actinomycetes;
            Propionibacteriales; Nocardioidaceae; Micropruina.
REFERENCE   1  (bases 1 to 9613)
  AUTHORS   Abdulkadir,N., Saraiva,J.P., Zhang,J., Stolte,S., Gillor,O.,
            Harms,H. and Rocha,U.
  TITLE     Genome-centric analyses of 165 metagenomes show that mobile genetic
            elements are crucial for the transmission of antimicrobial
            resistance genes to pathogens in activated sludge and wastewater
  JOURNAL   Microbiol Spectr 12 (3), e0291823 (2024)
   PUBMED   38289113
REFERENCE   2  (bases 1 to 9613)
  AUTHORS   Abdulkadir,N.
  TITLE     Direct Submission
  JOURNAL   Submitted (24-MAR-2023) Environmental Microbiology, Helmholtz
            Center for Environmental Research -UFZ, Permoserstr 15, Leipzig
            04318, Germany
COMMENT     The annotation was added by the NCBI Prokaryotic Genome Annotation
            Pipeline (PGAP). Information about PGAP can be found here:
            https://www.ncbi.nlm.nih.gov/genome/annotation_prok/
            
            ##Genome-Assembly-Data-START##
            Assembly Method        :: SPAdes v. 3.11.1
            Genome Representation  :: Full
            Expected Final Version :: Yes
            Genome Coverage        :: Not Applicable
            Sequencing Technology  :: Illumina MiSeq
            ##Genome-Assembly-Data-END##
            
            ##Genome-Annotation-Data-START##
            Annotation Provider               :: NCBI
            Annotation Date                   :: 05/04/2023 04:57:10
            Annotation Pipeline               :: NCBI Prokaryotic Genome
                                                 Annotation Pipeline (PGAP)
            Annotation Method                 :: Best-placed reference protein
                                                 set; GeneMarkS-2+
            Annotation Software revision      :: 6.5
            Features Annotated                :: Gene; CDS; rRNA; tRNA; ncRNA
            Genes (total)                     :: 2,776
            CDSs (total)                      :: 2,750
            Genes (coding)                    :: 2,714
            CDSs (with protein)               :: 2,714
            Genes (RNA)                       :: 26
            tRNAs                             :: 24
            ncRNAs                            :: 2
            Pseudo Genes (total)              :: 36
            CDSs (without protein)            :: 36
            Pseudo Genes (ambiguous residues) :: 0 of 36
            Pseudo Genes (frameshifted)       :: 2 of 36
            Pseudo Genes (incomplete)         :: 32 of 36
            Pseudo Genes (internal stop)      :: 2 of 36
            ##Genome-Annotation-Data-END##
FEATURES             Location/Qualifiers
     source          1..9613
                     /organism="Micropruina sp."
                     /mol_type="genomic DNA"
                     /submitter_seqid="NODE_3858_length_9613_cov_4.055974"
                     /isolate="AMR_MDS_2195"
                     /isolation_source="Activated sludge of municipal
                     wastewater treatment plants"
                     /db_xref="taxon:2737536"
                     /environmental_sample
                     /geo_loc_name="Canada"
                     /lat_lon="49.25 N 123.1 W"
                     /collection_date="2015-06-16"
                     /metagenome_source="wastewater metagenome"
                     /note="metagenomic"
     gene            <1..933
                     /locus_tag="PLF56_02285"
     CDS             <1..933
                     /locus_tag="PLF56_02285"
                     /inference="COORDINATES: protein motif:HMM:NF012798.2"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_function: GO:0003677 - DNA binding [Evidence IEA];
                     GO_process: GO:0006310 - DNA recombination [Evidence IEA];
                     GO_process: GO:0015074 - DNA integration [Evidence IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="tyrosine-type recombinase/integrase"
                     /protein_id="HOQ52422.1"
                     /translation="IDRGVHVDPRRQEVNVADWCDHWLQTKANLAPKTRERYEGILAV
                     QVRPKWHSVKLKNVTHSQVQAWLTGLRLAPASVRKVHRVFSQAMAYAVKDGRLGSNPA
                     AGVSLPRVESPEKLFLTHQQVKELAERCGDEYRVMVLFLAYTGVRWGEMAALRVRCLN
                     FVRMRASILQSVTPIRGVMTFGPTKGHECRDVPIPRHLVGALSDLVCDSGPDDLVFVG
                     QRGAPMRSQTFQRAALTQAAKDMRIAGFTPHMLRHTAASLAIAAGADVKVVQSMLGHK
                     SATLTLDLYGHLFGDRLDVVADAMEAARLQAISG"
     gene            2220..2750
                     /locus_tag="PLF56_02290"
     CDS             2220..2750
                     /locus_tag="PLF56_02290"
                     /inference="COORDINATES: ab initio
                     prediction:GeneMarkS-2+"
                     /note="Derived by automated computational analysis using
                     gene prediction method: GeneMarkS-2+."
                     /codon_start=1
                     /transl_table=11
                     /product="hypothetical protein"
                     /protein_id="HOQ52423.1"
                     /translation="MTVVNEVRGKIANEDLEREEGMISREVVLRVDRLIWTAESGKVE
                     APQEIRQQAAGWVFNNNNGTGERPFALTNASRLEVGHSYVTALDWVDDPCSKDVMKGS
                     WVGLGAFGTIPFDSGILGLGEVEGRIQDLRGVRVDIPTLRTDVAGKSVEELAAQLSTA
                     ERKKIAPHPTECEPED"
     gene            complement(2906..3433)
                     /locus_tag="PLF56_02295"
     CDS             complement(2906..3433)
                     /locus_tag="PLF56_02295"
                     /inference="COORDINATES: ab initio
                     prediction:GeneMarkS-2+"
                     /note="Derived by automated computational analysis using
                     gene prediction method: GeneMarkS-2+."
                     /codon_start=1
                     /transl_table=11
                     /product="hypothetical protein"
                     /protein_id="HOQ52424.1"
                     /translation="MNRTTSYQLTESDIERRNAFIAHWEAKHGLVPAYDLPSRPTDGR
                     YRIDERFRHSAETWYIDHFRMYISAYGLILIFSPYVKDLEKRLDDRVTPDELEAVKES
                     ASLNHAVFAVGRVCEGIYAQSSQTLYPDERRIHTVTVVFIIPLVGYAAEAADLAQQLA
                     KPLKFRAKDLHPNRR"
     gene            complement(3505..4191)
                     /locus_tag="PLF56_02300"
     CDS             complement(3505..4191)
                     /locus_tag="PLF56_02300"
                     /inference="COORDINATES: protein motif:HMM:NF012610.2"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="winged helix-turn-helix domain-containing
                     protein"
                     /protein_id="HOQ52425.1"
                     /translation="MAQGDHTSTGRLADRVADDLRARIAAGEYKPGDKLPSQNELAAS
                     YQVAKMTAYQAITQLREQGLVYARHGSGVYVTEPPVATSLFTAVSAAAATGHLTIDAL
                     APNARLLAAALRAPLARVVAGIDEAPAGRIRLMATTQDVEDYSYLQLCDDLEAHPTLT
                     LERRIIGLSSQFGFFLVNEADLFLGLSSEDHASLPPAVVAEQLIHAGRSDTAGISQIN
                     GVRDWFAQAW"
     gene            4320..4718
                     /locus_tag="PLF56_02305"
     CDS             4320..4718
                     /locus_tag="PLF56_02305"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_013865784.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="hypothetical protein"
                     /protein_id="HOQ52426.1"
                     /translation="MGVRGPFGVNFDEAFPHGVGIVGAVSAQRDFDKSSGDNSVQQLD
                     AATGKRIWVVDVMDFDPEARERTFKVKIIADVQPVPPAEVAGLPVRPVRLEGLSVTPY
                     LKEGMGRPRIAYSLRATGLAEVRRGDNKAA"
     gene            4720..6144
                     /locus_tag="PLF56_02310"
     CDS             4720..6144
                     /locus_tag="PLF56_02310"
                     /inference="COORDINATES: ab initio
                     prediction:GeneMarkS-2+"
                     /note="Derived by automated computational analysis using
                     gene prediction method: GeneMarkS-2+."
                     /codon_start=1
                     /transl_table=11
                     /product="hypothetical protein"
                     /protein_id="HOQ52427.1"
                     /translation="MSIVRAIRRELAWWLGGFARLLRRHPAGLLGFVGVALLYRALGV
                     DMAPVWGALAVLLGLAFWCRVSPASFRRLIGEPMRRRRVVRRVRQQWPSLMDSCGLVV
                     TDRVTGSSRRTFPALGRPRWDRHGDLTALVGLVNGQTVADFEAVADRLRTAVDARGLR
                     VVPNERRTGCRLVWSFTDSLAGLVDFVGPNPIAAGCSIDSVLIGRAESGDPFLLDLRV
                     STLTVGVAGAGKASVMWNVILGQAPNIRSGLVEYHGADLKGSMELTMGAALFTRLART
                     SAEAAVMLEETAAACDARAARLAGVTRLHQPTRTDPMVIVLIDELAVLVAYETDRELL
                     KRVDAALRRILAVGRAVGYYVFAFVQDPRKETIGMRHMFPQKVSLRLDEAAEVDMVLG
                     EGARRRGAEAHHISRSTPGVGFAVNETGEVERFRAAYVTDALIRLIASTHPAPRQVPI
                     VVPEPPEKPQRASRSRSRGQEDAA"
     gene            6141..6476
                     /locus_tag="PLF56_02315"
     CDS             6141..6476
                     /locus_tag="PLF56_02315"
                     /inference="COORDINATES: protein motif:HMM:NF014520.2"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="WhiB family transcriptional regulator"
                     /protein_id="HOQ52428.1"
                     /translation="MSNVKADRLLQFLRMEYRAIVVQEVDVNWRCPSWTAQTACSEGV
                     EPTPALCAACPVKAECLVTALVVDDPAPIRGGLTREDRTAAFLAIEQDAFHLDDWLRE
                     RAQELRMVS"
     gene            6476..7981
                     /locus_tag="PLF56_02320"
     CDS             6476..7981
                     /locus_tag="PLF56_02320"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_016667087.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="replication initiation protein"
                     /protein_id="HOQ52429.1"
                     /translation="MLATVGQITAGMARELAEAEGVCARPLLRRVTDRETGAVSRVAL
                     ACGSTREAVCPACAGKARRLRMQQCAEGWHRTDEPEADTGGITDNAAPGDTGSISDNT
                     DPDTGGITPGRRVRSTKRRQDVPDLPHVPMSDRTVGVVFSGRDGKEHRPSMFITLTLP
                     SYGAIHDGAPVTPATYDYRRTALDAMLFPRLLDQFWKTLRRTAGFNLQYFSAIEPQRR
                     LAPHLHAAIRGVIPRKVLRQVVTATYLQVWWPAFDHAIYVDELPRWTGTDYADPRTGE
                     LLPTWEESLDAIDNDPDAAPAHVMRFGSQLDAQGIVRGQPDADRAIRYLTKYLTKDVV
                     GTNDDDQEARRRAHIDRLHLELRYLPCSERCANWLRYGIQPKHAGPHLAPGFCNGKAH
                     RREHLGLGGRRVLVSRKWSSKTLARHRADRAEVVREALESAGLVAPDVERLSASVTTT
                     DGRARFVWEQIEGDHTTYAEVVMLAVAERRRWRTEYEAAREVQQGRSAMPP"
     gene            8191..8364
                     /locus_tag="PLF56_02325"
     CDS             8191..8364
                     /locus_tag="PLF56_02325"
                     /inference="COORDINATES: protein motif:HMM:NF024139.2"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_function: GO:0003677 - DNA binding [Evidence IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="helix-turn-helix domain-containing protein"
                     /protein_id="HOQ52430.1"
                     /translation="MADVAELLSVPIGTLYSWRHRNEGPRAYKMGRHVRYSRASVESW
                     LKGCLEEADHGLD"
     gene            8351..9460
                     /locus_tag="PLF56_02330"
     CDS             8351..9460
                     /locus_tag="PLF56_02330"
                     /inference="COORDINATES: protein motif:HMM:NF012798.2"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_function: GO:0003677 - DNA binding [Evidence IEA];
                     GO_process: GO:0006310 - DNA recombination [Evidence IEA];
                     GO_process: GO:0015074 - DNA integration [Evidence IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="tyrosine-type recombinase/integrase"
                     /protein_id="HOQ52431.1"
                     /translation="MASIERRELQRTDRSGRVRAVVRYKLRYRDASAREHSETFARLS
                     DAERRKAEIEVALGAGTWHDPRRGDVRLKDWAKEWLPTRHDLRPTTHARLVTTMERQV
                     LPRFGDVPLIKITNGAVRAWVADLLAEELSPATVRKAVFALRRCLDAAVEDRRISANP
                     AQKVSLPSEKAKRPRYLTRDEVERLVDEIPDRYRALVLVGAFAGLRWGEAIGLTRENV
                     DPLRSRITVMSTAIEVGGKVQLGQEPKTARSRRTVPVARSIMCRIANHLDQYVEPSPD
                     ALVFTTTTGGPLYRGTFGRQTWRRAVAAAGLDGFTFHGLRHSFVSILVAAGCNVREVS
                     EWAGHNSVAFTLTRYGGLYPDGSDAAVDRLDALLG"
BASE COUNT         1713 a         2851 c         3223 g         1826 t
ORIGIN      
        1 attgatcgcg gagtacacgt tgatccccga cgccaggagg tcaatgtcgc ggactggtgc
       61 gaccactggc tccagaccaa ggcgaacttg gcacccaaga cgcgtgagcg ctacgaaggc
      121 atcttggctg tgcaggtgcg gccgaaatgg cacagcgtga agctgaagaa tgtgacccac
      181 tcgcaggtgc aggcatggtt gaccggtctg aggttggcgc cggcatcagt gcgaaaggtc
      241 caccgggtct tctcccaggc gatggcgtac gcggtcaaag atgggcggct cgggtccaat
      301 ccggccgcgg gggtgagcct gcctcgggtt gagtctccgg agaagctctt cttgacacac
      361 cagcaagtca aggagctcgc cgaacggtgc ggggacgaat atcgggtgat ggtgctgttc
      421 cttgcctaca ccggcgttcg ctggggcgag atggccgcac tccgggtcag gtgcctgaac
      481 tttgtgcgga tgagggcaag tatcttgcag tcagtcacgc caatcagggg cgtgatgacg
      541 ttcgggccta cgaagggcca tgagtgtcga gacgtcccta tcccccgaca cttggtgggg
      601 gcactctccg atctggtctg cgacagcggc ccagacgacc ttgtcttcgt tggccagcgg
      661 ggagccccga tgcggtcgca gacgttccag cgcgccgctc tcacccaagc cgcgaaggat
      721 atgaggatcg cgggtttcac tccgcacatg ctgaggcaca cggccgcatc gttggccatc
      781 gccgccggcg cggacgtgaa ggtggtccag agcatgctcg gccacaagtc ggcaaccctc
      841 actttggacc tctacgggca cctgttcgga gaccggctcg atgtggtcgc ggacgccatg
      901 gaggcggccc gactgcaagc cataagtggc tgacatcccg ctcgtgcggg atttcgtgcg
      961 ggatggcggc tctcggaacg aaaagacggc ccagccaaac ttaggtttga ctaggccctt
     1021 tacccttgca gccccgacgg gattcgaacc cgcgctaccg ccttgagagg gcaagtggga
     1081 gcatcctgag ggggcaaaga gaacgaccgc cgagccttga ttgccttcgt tggcttgtca
     1141 cgctcagcgg gcgaactgac ccgcctgtgt ctcgtctgtg tccctgacgg ggttgcgtgg
     1201 gcgaccggtg tcgtcgagga gcgcatctgc ggttcctcga cttctaagga tttactcagg
     1261 ttaaatttcg ctctggctgg acggcccaaa ggatggtaaa ttgctccgat ggcggacctg
     1321 acgagatatc tgttgcgaac cctgtgcgtg ctcgtagcca cggccggcgt gctttcgttt
     1381 acgagcggct cctcgacggc ctcgcacact tatgacggga tagtcccgac taacacatac
     1441 aatacgtacc cgacgttcca cttctgcgag aatgcgagcg ggccaacaga tccggtggca
     1501 gtggtgtgtc agaccgatag ctccgggtgg tactggtatg cggatagcgg ggaccctggc
     1561 gagttggagg cggatgacaa agcatcggct caaagcgtct tgtcgagcca atacaatcct
     1621 acagatttga ctgtccacta cgatagcacg ccagtctttt caggttcggg ggaaacagac
     1681 ttgatcctag aggaagccga gtcggccaag ccgctcccaa gcagcgtgct tgggattacg
     1741 tggtgtaacg acgcggtcaa cggtgatcct cagtgggact gtgaccagac ttacgtgcgc
     1801 atagccaatc cctactaccg atcgttctca ggttcagtgg cgtgccacga aatgggccac
     1861 gcagttgggt tggtccacgg caatgacgcg tctccggtgc tggatcctgg tgatgcgcgc
     1921 ttaggctgca tggtcaacga ggacgagttt cccggctcgt tgggcgcggc gagcgctcat
     1981 ttgataaata ctgtctacta ggagtagaaa tgatggaacg aaaggcaacc gtcatggctc
     2041 tcgtagttgt ggcgctaggg gtctcaggct gtgggtacaa cgcgggaccc accgcttcgt
     2101 ccacgggggg tcgtcccacg cagaccgttg ctgcgccggt ggtgcttgga gaaggacgtg
     2161 accgcacagg gagtcgatcc gctagcgatt gggcatcgag tgcagagttc gtcgttgctg
     2221 tgaccgtggt caacgaggtg cggggcaaga ttgcgaatga ggatctggaa cgcgaagagg
     2281 gcatgatttc acgagaagtc gtgcttaggg ttgatcgctt gatatggacc gccgaaagcg
     2341 gcaaggttga ggcccctcaa gagattcggc agcaagcggc agggtgggtg ttcaacaata
     2401 acaatggcac cggtgagcgc ccattcgccc taaccaatgc atccaggctt gaagttggcc
     2461 acagctacgt aacggccctt gactgggtgg atgatccgtg ctctaaagat gtgatgaagg
     2521 ggtcatgggt cggtttgggt gcttttggga cgatcccatt tgattcaggg attctgggac
     2581 tcggggaggt tgaggggcgc attcaagacc ttcgcggggt tcgagtagat atcccaacct
     2641 tgaggacgga tgttgcgggc aagtcggtcg aggagttggc tgcacagctc tcgacagcgg
     2701 aacgcaagaa gatcgcacca catccgacgg agtgtgagcc tgaggactag gtggtttgga
     2761 actttgacct ggatacgtgc cgggggcacc agcgaggacc ggcccgcagc gagcgtagcg
     2821 agtgaggaca cggaccgcag cgagcgcggc gaagccgtgc cttgaatgag tagagccaga
     2881 ttcggcaaaa tggccatggt ttggcttagc gccggttggg gtgaaggtcc ttggcgcgga
     2941 atttcaatgg cttcgcgagt tgctgcgcca ggtcggccgc ttctgccgca taacctacga
     3001 gcggtataat gaacacgaca gtgacagtgt ggattcgcct ctcatccggg tatagcgtct
     3061 gagatgattg agcataaatg ccttcacata ccctgcccac cgcgaatacg gcatggttta
     3121 ggcttgccga ttccttgacg gcctcaagtt cgtcaggcgt gacgcggtcg tcgaggcgct
     3181 tctccaaatc tttgacatac gggctaaata tgagaatcag gccgtatgcg cttatataca
     3241 tgcggaagtg atcgatatac caagtctctg ccgagtgccg aaaacgctcg tcaatccgat
     3301 accgaccatc tgttgggcgg ctgggcagat cgtaggcagg cacaagtccg tgcttggctt
     3361 cccaatgggc gatgaatgca ttgcgccgtt cgatgtcaga ttctgtgagc tgataggacg
     3421 ttgttctgtt cacgacggct tctccttggg gttgggcgct tcgcgtcaga gttcgcgtgg
     3481 caggcagcat agtctcttcg agattcacca agcttgggcg aaccaatctc gcactccatt
     3541 gatctgactg ataccggcgg tatcacttct gccagcgtga atcagttgtt ctgccaccac
     3601 tgccggtggg agtgaggcgt gatcctccga gctgaggccg aggaacaggt ctgcctcgtt
     3661 gaccaggaag aaaccgaact gggagcttag cccgatgatg cgcctctcca gcgtcagcgt
     3721 cgggtgcgcc tccaggtcgt cgcacagttg caggtatgag tagtcctcga cgtcttgcgt
     3781 agtggccatg aggcggattc ggccggccgg cgcctcgtca atcccagcta ccacccgcgc
     3841 caggggtgcg cggagcgcgg ccgcgaggag tcgagcgttg ggcgcgagcg cgtcgattgt
     3901 taggtgaccc gtcgcggcgg ccgccgagac agcggtgaac agcgaagttg ccaccggcgg
     3961 ctcagtgacg tagaccccgc tgccgtggcg tgcgtagacc aggccttgtt cgcgaagctg
     4021 ggtgatcgct tgatacgccg tcatcttggc aacttggtag ctggcggcga gctcgttctg
     4081 cgacggcagc ttgtcgccgg gcttgtactc ccccgccgcg atgcgtgcgc gcaagtcgtc
     4141 ggccacccga tcagcgaggc gaccggttga tgtgtgatcg ccctgagcca tgtcagagag
     4201 tctagaagtg cagttcgtcc tcagcgacac acatctaagc actcttgaca ctgtttagat
     4261 cagtggtgca tagtgtttgg aacagtgatg gtcactgctt agaacactag gaggatgcga
     4321 tgggcgtcag aggaccgttc ggtgtgaact tcgatgaggc gtttccgcac ggcgtgggaa
     4381 tcgtcggtgc ggtgagcgcg caaagagact tcgacaagtc cagcggggac aactcggtgc
     4441 agcaactcga cgccgcaacc ggcaagcgca tctgggtcgt cgatgtcatg gacttcgacc
     4501 cggaggcccg cgaacgcacg ttcaaggtca agatcatcgc tgacgtacag ccggtgccgc
     4561 ccgccgaggt tgccggcttg ccggtccgtc ccgttcggct tgaaggactc tcggtgaccc
     4621 cttacctcaa ggaaggcatg gggcgacccc ggatcgcgta ctccctgcgg gcgaccggct
     4681 tggccgaggt tcgccgtggc gacaacaagg cggcgtgagg tgagcatcgt ccgtgcgatt
     4741 cgtcgcgagc tcgcgtggtg gctgggcggt ttcgctcggc tgctgcgccg gcacccggct
     4801 gggttgctgg ggttcgtggg cgttgccctg ctgtaccggg cgctgggtgt cgacatggca
     4861 cctgtttggg gtgccctcgc ggttttgctc gggctggcct tctggtgccg cgtgtcgccg
     4921 gcctcgttcc ggcggctaat cggtgagccg atgcgtcggc gccgggtggt tcggcgggtt
     4981 cgacagcagt ggccgtcgct gatggactcc tgcggcctcg ttgtgaccga ccgcgtgacc
     5041 ggttcatcgc gtcggacgtt tcccgccttg ggccggcccc gttgggatcg gcatggggac
     5101 ctgaccgctt tggtgggttt ggtgaatggc cagactgtcg cagatttcga ggcggttgct
     5161 gatcggctcc gcacggcagt cgatgctcgg gggttgcgcg tggttccgaa cgaacgccgg
     5221 acgggttgcc gcctggtttg gtcgttcaca gactcgttgg ccggcctggt cgatttcgtc
     5281 ggcccgaacc cgatcgcggc tggatgctcg atcgattcgg ttctgatcgg gcgcgccgag
     5341 tcgggcgatc cgttcctgct cgatctgcgg gtgtcgacac tgaccgtcgg cgtggccggt
     5401 gcgggcaagg cgtcagtgat gtggaacgtg atccttggtc aggcaccgaa cattcgctcg
     5461 ggtctggtcg agtatcacgg cgcggacttg aaggggtcaa tggagctgac catgggggca
     5521 gcgctgttca cccgcttggc tcgaacgtca gctgaggcgg ccgtcatgct cgaagagacc
     5581 gccgccgcct gtgatgctcg cgcggctcgc ctggctggag tgaccagact gcatcagccg
     5641 acgcggaccg acccgatggt gatcgtcttg attgacgagc ttgccgttct ggtcgcctat
     5701 gagaccgacc gagagctgtt gaagcgggtc gatgccgcgc tgcggcgcat tctcgcggta
     5761 ggccgagcgg tcggctacta cgtgttcgcg ttcgtgcaag acccgcgcaa agagaccatc
     5821 ggcatgcggc acatgttccc gcagaaggta tcgctgcgcc tggacgaggc tgccgaggtt
     5881 gacatggttc ttggtgaggg agcccgccgg cggggtgccg aagcgcatca catcagccgc
     5941 tcgacacctg gcgtgggttt cgcagtgaat gagaccggag aagtcgagcg gttccgagcc
     6001 gcatacgtca ccgatgccct gatccggctg atcgcctcca cccaccccgc accacgccag
     6061 gtgcccatcg tggtgcccga gccgccagag aagccgcagc gcgccagccg cagccgttcg
     6121 cgcgggcagg aggacgcagc atgagcaacg tcaaagctga tcggctgctg caattcctgc
     6181 gcatggagta ccgggccatc gtcgtccagg aggtcgacgt gaactggcgc tgcccctcct
     6241 ggaccgccca gacagcgtgc tccgaaggag tcgaaccgac gccggccctg tgcgctgcct
     6301 gccccgtgaa ggccgaatgc ctcgtcaccg cattggtggt ggacgacccc gcaccgatcc
     6361 gcggaggcct gacccgcgaa gaccgcaccg ccgcattcct ggcaatcgag caagacgcct
     6421 tccacctgga cgactggctg cgggagcgtg cccaagaact ccgcatggtg tcgtgatgct
     6481 cgccaccgtg ggacagatca ccgccggcat ggcccgcgaa ttggccgagg ccgaaggcgt
     6541 atgcgcccga ccgctgctgc gccgcgtgac cgaccgtgaa accggcgcgg tgtcgcgggt
     6601 ggcgttggcg tgcgggtcga cccgtgaggc agtatgcccg gcctgcgctg gcaaggctcg
     6661 acggctcagg atgcagcaat gcgccgaggg ctggcatcgc accgacgaac cagaggctga
     6721 taccggcggt atcaccgaca acgccgcacc gggtgatacc ggcagtatca gcgacaacac
     6781 cgaccctgat actggcggta tcactcccgg cagacgtgtc cggtcgacca agcgccgcca
     6841 agacgtgcct gatcttccgc acgtgccaat gagtgaccgc acggtcgggg tggtgttcag
     6901 cggccgggac ggcaaggagc accgcccgtc gatgttcatc acgctcacgt tgccgtcgta
     6961 cggcgcgatt cacgacgggg caccagtcac gccggccacc tacgactatc ggcggacggc
     7021 gttggacgcg atgctgttcc cgcgactgct cgatcagttc tggaagacac tgcgccgaac
     7081 ggcgggtttc aacctgcagt acttctcggc catcgaaccg cagcgccggc tagctccgca
     7141 tctgcatgcc gcgatccggg gtgtcattcc acgcaaggtg ctgcgccagg tagtcaccgc
     7201 cacgtatctg caagtctggt ggcccgcgtt cgatcacgcc atctacgttg acgagctgcc
     7261 ccgctggaca ggcaccgact acgccgaccc tcgcaccggt gagctgctgc ccacctggga
     7321 ggaatccctc gacgccatcg acaacgatcc cgacgcggcg ccggcgcacg tgatgagatt
     7381 cggcagccag ctcgacgctc aagggatcgt gcgcggccag cccgacgccg accgcgccat
     7441 tcgctatttg acgaagtacc tcaccaagga cgtggtcggc accaacgacg acgaccaaga
     7501 agcgcgccgc cgcgcacaca tcgaccggct gcatctcgag ctccgctacc tgccctgctc
     7561 cgaacgctgc gcgaactggc tgcgctacgg catccaaccc aagcacgccg gtccccacct
     7621 ggcgccgggc ttctgtaacg gcaaagcgca tcggcgcgag cacctggggc ttggtggccg
     7681 gcgcgtgttg gtgtcgcgca aatggtcgtc caagacactc gccaggcacc gggcagaccg
     7741 cgccgaagtc gtacgcgaag cactggaatc ggccggcttg gtcgcccccg acgtggaacg
     7801 gctctcggcc agcgttacca ccaccgatgg gcgtgcccgg ttcgtgtggg aacagatcga
     7861 aggcgaccac accacctacg ccgaggtagt catgctggcg gtcgcagaac ggcgacgctg
     7921 gcgaaccgaa tacgaagcag cccgtgaagt tcagcaaggc cgttcagcaa tgccgccgtg
     7981 accgcagacc gcttcgacct ccccgatgac tgcccaccgt gggtcagggc tgaccggtca
     8041 agggagagcg aagcgaatca cgcagtgaca cgaagtgccc ttgacggggc agacatgacc
     8101 caccaattcc ttcccaacgt cggggagggc gaagcaccta cgttcccaga ccccaggggg
     8161 tggatatccg tggctgagaa gctgttgacg atggccgatg tggctgagtt gttgtcggtg
     8221 ccgattggca cgttgtattc gtggcggcac cgtaacgagg gtccgcgcgc ttacaagatg
     8281 ggtcggcacg tcaggtatag ccgggcttcg gttgagtcgt ggttgaaggg gtgccttgag
     8341 gaggcagatc atggcctcga ttgagcggcg cgagcttcaa cgaacagatc ggtcgggccg
     8401 ggttcgcgct gttgtccggt acaagcttcg ttatcgggac gcgtccgctc gggagcactc
     8461 cgagaccttc gctcggctga gtgacgcgga gcggcgcaag gcagagattg aggtcgccct
     8521 cggcgccggc acctggcacg accctcgccg cggagacgtt cgtttgaagg actgggcgaa
     8581 ggagtggctg ccgacccgtc acgatctgcg gcccaccact catgcccgct tggtgacgac
     8641 gatggaacgc caggtgctcc ctcgctttgg cgatgtgccg ctgatcaaga tcacgaacgg
     8701 cgcggtgcgg gcatgggtgg ctgatctgtt ggccgaggaa ctgtcgccgg cgactgtgcg
     8761 taaggctgtg ttcgccttgc ggcggtgcct ggatgctgcc gttgaggacc gtcgcatatc
     8821 ggccaaccct gctcagaagg tatcgcttcc gtctgagaag gcgaagcggc ctcgatacct
     8881 aaccagggac gaggtggagc gcctcgttga tgaaatccct gatcggtacc gcgcgctcgt
     8941 gctggtggga gccttcgccg gcctgcgatg gggtgaagcg attgggctga cccgcgagaa
     9001 cgtcgacccg ctgcgatcac ggatcacggt gatgtcgacg gccattgagg tcggcgggaa
     9061 ggtgcagctc ggccaggagc ccaagaccgc taggtcgcgt cgcacggtgc cggttgcgcg
     9121 ctcgatcatg tgtcgaatcg cgaaccacct cgatcagtat gttgagccgt cgccggatgc
     9181 tctcgtgttc acgacgacca cgggcgggcc tctgtatagg ggcaccttcg gtcgtcagac
     9241 ttggaggcga gcggtcgccg cggcgggtct ggacggcttc acctttcacg gcttgcggca
     9301 cagcttcgtg tcgatcttgg tcgccgccgg ctgcaacgtg cgcgaggtct cggagtgggc
     9361 gggccacaac agcgttgcct tcacactcac ccgctacggc ggtctgtatc ccgacggctc
     9421 agacgccgcc gtcgaccgcc tcgacgcgct actcggctga gcgttcgtgt cccgtacgtg
     9481 tcccgagggc ttgaggttgg aacatgcgaa gcggctcccg ccgggtgtta tacccagtgg
     9541 aagccgcttt cgtgccttgc agccccgacg ggattcgaac ccgcgctacc gccttgagag
     9601 ggcggcgtgc tag
//
LOCUS       DAOSUG010000039         9506 bp    DNA     linear   ENV 10-JUN-2024
DEFINITION  MAG TPA_asm: Micropruina sp. isolate AMR_MDS_2195
            NODE_3893_length_9506_cov_5.289916, whole genome shotgun sequence.
ACCESSION   DAOSUG010000039 DAOSUG010000000
VERSION     DAOSUG010000039.1
DBLINK      BioProject: PRJNA925477
            BioSample: SAMN32864262
            Sequence Read Archive: SRR4236673
KEYWORDS    WGS; ENV; Metagenome Assembled Genome; MAG; Third Party Data; TPA;
            TPA:assembly.
SOURCE      Micropruina sp. (wastewater metagenome)
  ORGANISM  Micropruina sp.
            Bacteria; Bacillati; Actinomycetota; Actinomycetes;
            Propionibacteriales; Nocardioidaceae; Micropruina.
REFERENCE   1  (bases 1 to 9506)
  AUTHORS   Abdulkadir,N., Saraiva,J.P., Zhang,J., Stolte,S., Gillor,O.,
            Harms,H. and Rocha,U.
  TITLE     Genome-centric analyses of 165 metagenomes show that mobile genetic
            elements are crucial for the transmission of antimicrobial
            resistance genes to pathogens in activated sludge and wastewater
  JOURNAL   Microbiol Spectr 12 (3), e0291823 (2024)
   PUBMED   38289113
REFERENCE   2  (bases 1 to 9506)
  AUTHORS   Abdulkadir,N.
  TITLE     Direct Submission
  JOURNAL   Submitted (24-MAR-2023) Environmental Microbiology, Helmholtz
            Center for Environmental Research -UFZ, Permoserstr 15, Leipzig
            04318, Germany
COMMENT     The annotation was added by the NCBI Prokaryotic Genome Annotation
            Pipeline (PGAP). Information about PGAP can be found here:
            https://www.ncbi.nlm.nih.gov/genome/annotation_prok/
            
            ##Genome-Assembly-Data-START##
            Assembly Method        :: SPAdes v. 3.11.1
            Genome Representation  :: Full
            Expected Final Version :: Yes
            Genome Coverage        :: Not Applicable
            Sequencing Technology  :: Illumina MiSeq
            ##Genome-Assembly-Data-END##
            
            ##Genome-Annotation-Data-START##
            Annotation Provider               :: NCBI
            Annotation Date                   :: 05/04/2023 04:57:10
            Annotation Pipeline               :: NCBI Prokaryotic Genome
                                                 Annotation Pipeline (PGAP)
            Annotation Method                 :: Best-placed reference protein
                                                 set; GeneMarkS-2+
            Annotation Software revision      :: 6.5
            Features Annotated                :: Gene; CDS; rRNA; tRNA; ncRNA
            Genes (total)                     :: 2,776
            CDSs (total)                      :: 2,750
            Genes (coding)                    :: 2,714
            CDSs (with protein)               :: 2,714
            Genes (RNA)                       :: 26
            tRNAs                             :: 24
            ncRNAs                            :: 2
            Pseudo Genes (total)              :: 36
            CDSs (without protein)            :: 36
            Pseudo Genes (ambiguous residues) :: 0 of 36
            Pseudo Genes (frameshifted)       :: 2 of 36
            Pseudo Genes (incomplete)         :: 32 of 36
            Pseudo Genes (internal stop)      :: 2 of 36
            ##Genome-Annotation-Data-END##
FEATURES             Location/Qualifiers
     source          1..9506
                     /organism="Micropruina sp."
                     /mol_type="genomic DNA"
                     /submitter_seqid="NODE_3893_length_9506_cov_5.289916"
                     /isolate="AMR_MDS_2195"
                     /isolation_source="Activated sludge of municipal
                     wastewater treatment plants"
                     /db_xref="taxon:2737536"
                     /environmental_sample
                     /geo_loc_name="Canada"
                     /lat_lon="49.25 N 123.1 W"
                     /collection_date="2015-06-16"
                     /metagenome_source="wastewater metagenome"
                     /note="metagenomic"
     gene            269..910
                     /locus_tag="PLF56_02335"
     CDS             269..910
                     /locus_tag="PLF56_02335"
                     /inference="COORDINATES: protein motif:HMM:NF017309.2"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_component: GO:0016020 - membrane [Evidence IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="lipoprotein LpqH"
                     /protein_id="HOQ52432.1"
                     /translation="MTARTWYRSVTPLAIVAVALSWTGCAKTPPALTPTAQASATVAV
                     PESPSATDSPAASASASPTASATSASPSASATASATATSGPAVKATGSLKLFANASKK
                     LTGSCQNKAGAPTLTVADKKNDFFQTVDVTLVLTAGKKSVASITIDLGEDSELITRKL
                     TYNAGQPASGTSAKLAVRGATYTVAGTLASAENGSPAGTMPVILTVACAGTSW"
     gene            complement(980..3418)
                     /locus_tag="PLF56_02340"
     CDS             complement(980..3418)
                     /locus_tag="PLF56_02340"
                     /inference="COORDINATES: protein motif:HMM:NF013954.2"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="peptidoglycan-binding protein"
                     /protein_id="HOQ52433.1"
                     /translation="MLSLTVSLTLAATVALVDVSGVTAATISANAKDKFIAGVVAPAQ
                     AAQRTYGVPASVSIAQAIEASDWGTSSVAKNAKNYFDTRCSASMTAAQFASLADAQVG
                     KPYVLGAEAAISNTDPAKFDCSELVEWLFGRSGNRITDLAAAQYNVTKKVTGSPKVGD
                     LVFLRNNPARSNGIGHVAVLTKKLSNGDWRVIEARGRASGVVRTTLSYWKKRRYYAGL
                     RRLSSFVLAGKDGVTASAASTLQAGCVTVSGTRYAKYTSVKNSFAAHAVAVSQDSAYS
                     AARAVIGDQPAYVTAIAKVVQPKNAAGYAQRINELIAQYDLRDYDVVPFNLVLTSGAS
                     GAKVTATQHLLGAAGYTVSTTGTFDSATVSAVKKFQKAKGLESDGEAGPKTLTTLTTK
                     ISSGARGGSVTALSTLLSAVGYRTGSPSSFGTDTVAAVKAFQAAVGRPVTGSVDDNTW
                     AALYMTLTAPTPKIAGKAVVGQKLTAGAGTWGPGSVSLRYQWLRGGTPISGATKTTYT
                     PGVADAGYALSVRVTGTKRLYTTTARTSAATGTVPLLTLTARPVPTISGKALVGSTLT
                     AQTGTWAPAPVALAYQWYRGGVAIGGATRATYTITAADAGSALTVAVTATKTGYAPVT
                     TQSKPSASVPRTLTGGTPTITGGAKVGSTLMAVPGAWSPATAKLTYQWYAGSTAIKGA
                     TKATYQPQPADVGKTITVQVTGTLAGYITTSRASAATAAVVKGTLSPKTPTISGTRKS
                     GRWLTAKGGNWGPGTVKLSYQWYRGSDKVKGATKTGYKLTSKDKGKTVSVVVTGTKSG
                     YAAQSQKVGVKIAK"
     gene            complement(4290..6359)
                     /gene="tkt"
                     /locus_tag="PLF56_02345"
     CDS             complement(4290..6359)
                     /gene="tkt"
                     /locus_tag="PLF56_02345"
                     /EC_number="2.2.1.1"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_016666553.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_component: GO:0005737 - cytoplasm [Evidence IEA];
                     GO_function: GO:0004802 - transketolase activity [Evidence
                     IEA];
                     GO_process: GO:0006098 - pentose-phosphate shunt [Evidence
                     IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="transketolase"
                     /protein_id="HOQ52434.1"
                     /translation="MSTLTWTEQDAKAVDIARVLAADAVEKVGNGHPGTAVSLAPVAY
                     LLYAKVMKVDPAEPRWLGRDRFVLSAGHSSMTQYAQLYLNGFGLELNDMQSLRTWDSL
                     TPGHPEFGLTKGVECTTGPLGAGVANAVGFAMSARRSRELYDPQATPGESVFDHNVYA
                     IAGDGCMQEGVSGEASSMAATLELGNLFLIYDDNRISIEGDTKIAFSEDVQARYDAYG
                     WDTYHVDFTNGFTTYEEKVAELYDVLERAKAVTDKPHFIKVTTIIGWPLPNKAGDHSV
                     HGSKLGADEIAALKAELGFENEPFAIDTSIVDAVRANAAERAKALHDEWEPRYETWRS
                     ANPTQAELLDRVLAREVPADLDAALPVFEPGSMATRAASGKVLSALAAPVPELWGGSA
                     DLAGSNNTTMAGVNSFIPSDRSSHEFTGAPGGRTLHFGIREHAMGNIANGITMDGLTR
                     VYCATFLVFSDYMRTTPRLAALSHAPTTFVWTHDSVGVGEDGPTHQPIEHVMSLRLMP
                     NLDVVRPGDANETAHAWAQILRTKNRPAALILSRQNLPILDRSDESGFAAASNTALGG
                     YVLKEASGEPKVVLVGTGSELQVAVEAAKTLEADGIPARVVSLPCVEWFQAQDDEYRE
                     QVLPASVPKVVIEAGVPVGWKDLIGANTEAIGIDHFGASADGALLFEKFGITADAAVA
                     KAKALMG"
     gene            complement(6683..8011)
                     /locus_tag="PLF56_02350"
     CDS             complement(6683..8011)
                     /locus_tag="PLF56_02350"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_016670293.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_function: GO:0022857 - transmembrane transporter
                     activity [Evidence IEA];
                     GO_process: GO:0055085 - transmembrane transport [Evidence
                     IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="MFS transporter"
                     /protein_id="HOQ52435.1"
                     /translation="MTDVSNATSLTRAERLDRLPFTRKHGKLLLGSGLGWALDAMDVG
                     LISFVMAALAAQWKLDSTTLSWIGSIGFVGMAIGASLGGLLADRIGRRQVFALTLLLY
                     GVATGASALVTSVAMLIALRFIVGIGLGAELPVASTLVSEYAPKAIRGRVVVALEAFW
                     AVGWILAALIGYLVVPSSPDGWRWALAVGVIPTAYAAAIRWGLPESVRFLESKGRIDQ
                     AEAAVRSFEEAAGVEPVPSPDAPPARATPTDSIWSPAYRARTAALWAVWFCINLSYYG
                     AFIWIPTLLVQRGFPLVRSFEFTLIITLAQIPGYAVAAWLIETWGRRITLATFLAGSA
                     LAAAAFGFATTETLIIVTGCALSFFNLGAWGALYAVGPELYPTAVRGKGTGAAAAFGR
                     IASMLAPLSVPLLLGAGGMVLVFGVFGVAFLLAAGAALALPDQTGRALES"
     gene            8086..8466
                     /locus_tag="PLF56_02355"
     CDS             8086..8466
                     /locus_tag="PLF56_02355"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_012920648.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="hypothetical protein"
                     /protein_id="HOQ52436.1"
                     /translation="MNAQPTDHRTATLMGAGLIGMGALHFAAPKGFDAIIPQELPVAP
                     RTLTYASGVAELAIGAGLLHPASRKVSALAAAALFVAVYPANLNTVRVVADQPLPYRL
                     LAWARLPLQVPLIVAAWRIFRRAE"
     gene            complement(8624..>9506)
                     /locus_tag="PLF56_02360"
     CDS             complement(8624..>9506)
                     /locus_tag="PLF56_02360"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_016455900.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=2
                     /transl_table=11
                     /product="alcohol dehydrogenase catalytic
                     domain-containing protein"
                     /protein_id="HOQ52437.1"
                     /translation="AGVRQGVIMGHEIAGRVAALGDGVTGYEVGQQAAPSIVISCNHC
                     RVCLLGLEHLCENLQLIGYEIDGGLADYCLIPARAVAQGNLVTTRTRVKPTHLALAEP
                     LSCCINALNQYRVQPGDVVAILGAGPIGLLHLQLAQASGASQVIVSNRSVARRAPAAA
                     LGAVAVGPDEVKQAVLDATGGLGADVVVICIGEPSLASVALDLARPGGRVSYFAGFPK
                     GSTAEVDVNLLHYKELEVTGGSNARRRDVQAAVRLLESGHLNADAIVTNTFGLDEAPA
                     AYDAVRDRLGVKIAVTP"
BASE COUNT         1464 a         3244 c         3279 g         1519 t
ORIGIN      
        1 gacagcttgc ccagaggatg catgcctgcc agcgtatgcg gccccgtgcg gccacgtggt
       61 cggtgcccgc gctgcgcgac cccggacgcc gtcgaggcac acgctgatcc atcgccggtc
      121 gaacctgccg aaacggtcat cagcagaggg ctcggcaggc tcaaccaacg gacgtgaggg
      181 tgatcggctc gtccctgggc acaccaatca cgctctccga gcgagtcgcg acgccgatgt
      241 gggggtgcga ggttccagca tggacggtgt gactgcacgc acctggtatc gatccgtgac
      301 gccgctggcc attgtggccg tggccctgtc gtggacgggc tgtgcgaaga ccccgccagc
      361 gctcacgccg acggcgcagg ccagcgcgac ggtcgccgta ccggaatcac cgtcggccac
      421 ggactcgccg gcggctagcg cgtccgcctc ccccaccgcg tcggcgacca gcgcttcgcc
      481 cagtgcgtcc gcgacggcga gcgcgacggc cacgtccggg ccggccgtga aggcgacagg
      541 ttcgctgaag ctgttcgcga acgccagcaa gaagctcacc ggcagctgcc agaacaaggc
      601 cggcgccccg accctgaccg tcgcggacaa gaagaacgac ttctttcaga ccgtggacgt
      661 gacgctggtg ctgacggcgg gcaagaagag cgtggcgagc atcaccatcg acctgggcga
      721 ggactccgag ctgatcaccc gcaagctcac ctacaacgct gggcaacctg cgtcgggaac
      781 gtccgcgaag cttgcggtca ggggggcgac ctacacggtg gccggcacgt tggccagtgc
      841 cgagaacggc tcccccgcgg gcaccatgcc ggttatcctc accgtggcct gtgccggcac
      901 gagttggtga ggccggcccc gaccacgggc ccggggtgtg tccttcgaat catttgccgt
      961 tcgggcacac accactggtt cacttggcga tcttgacgcc caccttctgc gactgcgctg
     1021 catagccaga cttggttccg gtgacgacca cgctgacggt cttgcccttg tctttgctgg
     1081 tcagcttgta cccggtcttg gtcgctccct tcaccttgtc gctgccgcgg taccactggt
     1141 agctcagctt caccgtgccg gggccccagt tgccgccctt ggccgtgagc caacggcccg
     1201 acttgcgcgt accggagata gtcggggtct tcgggctcag tgtgcccttc acgaccgcag
     1261 cggtggctgc tgaggcacgc gaggtcgtga tgtatccggc cagggttccg gtcacctgca
     1321 cagtgatggt cttgccgacg tccgcgggct gcggttggta ggtggccttg gtagcgccct
     1381 tgatggcggt cgaccccgcg taccactggt aggtgagctt cgcggtggcc ggcgaccagg
     1441 cacccggtac ggccatgagc gttgagccga ccttcgcgcc acccgtgatc gtcggggtgc
     1501 cgccggtcag cgtgcgcggc accgaagcgc tgggcttcga ttgtgtggtc accggtgcgt
     1561 acccggtctt cgtcgcggtc accgcaaccg tgagcgccga gcccgcatcg gcggccgtga
     1621 tcgtgtaggt cgcccgggtg gcacccccga tcgcgacgcc gccgcggtac cactggtacg
     1681 ccaacgccac tggggccggt gcccacgtgc ccgtctgcgc ggtcagtgtc gaacccacca
     1741 gggccttgcc gctgatcgtg ggaacaggcc ttgcggtcag cgtgagcaac ggcaccgtgc
     1801 cggtggccgc cgaggtgcgt gcggtggtgg tgtacaggcg cttggtgccg gtgacccgca
     1861 cgctcaacgc gtagccggcg tcggcgacac ccggggtgta ggtggtcttg gtcgccccac
     1921 tgatcggtgt tccgccgcgc aaccactggt accgcaggct caccgagccg ggcccccagg
     1981 tgccggcgcc ggcggtcagc ttctgcccca ccaccgcctt gcccgcgatc ttcggggtcg
     2041 gtgcggtcag cgtcatgtac agggcggccc aggtgttgtc gtccacagaa ccggtcaccg
     2101 gacgtccgac ggccgcttgg aacgccttga ccgccgcgac ggtgtcagta ccgaaactgg
     2161 acggggagcc ggttctgtac ccgaccgcgc tcagcagagt gctcagcgcg gtcaccgaac
     2221 caccacgcgc accgctggaa atcttggtgg tcagggtggt cagggtcttg ggtccggcct
     2281 cgccgtcgct ctcgagaccc ttggccttct ggaacttctt caccgccgac accgtcgcag
     2341 agtcgaacgt gcccgtggtc gagacggtgt agccggccgc ccccagcagg tgctgcgtgg
     2401 cagtcacctt ggcacccgag gcgcccgagg tgagcaccag gttgaacggc accacgtcgt
     2461 agtcgcgcag gtcgtactgg gcgatcagct cgttgatcct ctgggcgtac ccggcggcgt
     2521 tcttcggctg cacgaccttg gcgatcgccg tcacgtacgc gggctggtca ccgatcaccg
     2581 cccgggcggc gctataggcg gaatcctgcg acaccgcgac cgcgtgggcg gcgaacgagt
     2641 tcttcaccga ggtgtatttg gcgtagcggg tgcccgaaac cgtcacgcag cccgcctgca
     2701 gggtgctcgc ggccgacgcc gtgacaccgt ccttgccggc cagcacgaag ctcgacagcc
     2761 ggcgcaaccc ggcgtagtag cggcgcttct tccagtagct cagcgtggtg cgcaccacgc
     2821 cggaggcccg gccgcgggcc tcgatgacgc gccaatcacc gttgctcagt ttcttggtca
     2881 gcacggccac gtgaccgatg ccgttggagc gggccgggtt gttgcgcagg aacaccaggt
     2941 cgcccacctt gggtgagccg gtgaccttct tggtgacgtt gtactgggcg gccgccagat
     3001 cggtgatccg gttgccggag cgtccgaaca gccactcgac gagttcggag cagtcgaatt
     3061 tggctgggtc ggtgttgctg atcgcagcct cggcgcccag cacgtacggc ttgcccacct
     3121 gcgcgtccgc aagactggcg aactgggcgg cggtcatcga ggcggagcag cgggtgtcga
     3181 agtagttctt ggcgttcttg gccaccgacg aggtgcccca atcgctggcc tcgatggcct
     3241 gcgcgatcga caccgaggcg ggcacaccgt aggttcgctg cgccgcctgg gccggtgcca
     3301 cgacgccggc gatgaacttg tctttggcgt tcgcgctgat ggtcgccgcc gtgacaccgg
     3361 acacgtcgac cagcgccacc gtcgcggcga gggtcaggct gacggtcagg gacagcacct
     3421 tcgaggggaa tcgcacaatg aactagctac cgacgcgccg cacgcgtcgg caagggtcca
     3481 cttgagggtt gctgctcagc ctttcttcag aaatcgcgtg cgactagggg ttttggttac
     3541 tggttcgctc aagttgcgct taagggttca ctcgagggtt gcatcccggg cccgaatagg
     3601 cctcgggccg gatccccgat gtcctcctcg tcgttcgccc tgcgatcacc cgagccggcc
     3661 acatgctggc gggacgcacc cggggtcgcc agacgcgccc gtccggagcg ggtgggtttt
     3721 gccaccgact gcggtggcaa aacccacccg ctcgcaatcc cacgcgcaac caggccgagg
     3781 taccggtggt cgcggacttg gccaaggtgc ctgtgtcgaa gcgcctgttc gggtcggggt
     3841 ggtgattgga tgacgtttcg tcacaccctg ccgctccagc ctggtctgca tcgcttgacg
     3901 aacgagtggg gacgcgcggc tctccgggtg acagaacggt cagtgtggac ggggcggtgc
     3961 acacgcctgc tcagcgcggc gggcgtccag gcccgaggtg agcaggatgt gaaccgaggt
     4021 gggggcgctt ccgggctcga aaaatctgcc ctccatcggg cccgccgcga tccatcgagc
     4081 ccggttcctg gccgcaacga ggagtacccg gaagctctcc cctggccacc cgcgctcgga
     4141 cgacctccgc gttgagcgga actcttgaac agggttggga gaccgtcccc cactccgttc
     4201 agggggctga gcaagacgcg tgaacggagt tgggaaccgt ccccaactcc gttcacgtac
     4261 gggattcacc cggtcgtcgg cccgagcgat cagcccatca aggccttcgc cttggcgacc
     4321 gcggcgtcgg cggtgatgcc gaacttctcg aacagcagcg cgccgtcggc ggaggcgccg
     4381 aagtggtcga tgccgatcgc ctcggtgttg gcgccgatca gatccttcca accgaccggc
     4441 acaccggcct cgatgaccac tttcggcacg gacgccggca gcacctgctc gcggtactcg
     4501 tcgtcctggg cctggaacca ctccacgcag ggcagcgaga ccacccgggc cgggatgccg
     4561 tccgcctcaa gggtcttggc cgcctcgacg gccacctgca gctcggaacc cgtgccgacc
     4621 agcaccacct tcggctcgcc cgaggcctcc ttgagcacgt acccgcccaa tgcggtgttg
     4681 gacgccgcgg cgaagcccga ctcgtccgac cgatcgagaa tgggcaggtt ctgccgcgac
     4741 aggatcagcg cggccggacg gttcttggtg cgtaggatct gcgcccacgc gtgcgcggtt
     4801 tcgttggcgt cgccggggcg caccacgtcg aggttcggca tcagccgcag gctcatcacg
     4861 tgttcgatcg gctggtgggt ggggccgtcc tcgccgacgc cgaccgagtc gtgggtccag
     4921 acgaacgtgg tcggcgcatg cgacagcgcg gccagcctcg gggtggtgcg catgtagtcg
     4981 ctgaagacca ggaaggtcgc gcagtagacc cgggtcaggc cgtccatcgt gatgccgttg
     5041 gcgatgttgc ccatggcgtg ttcgcggatg ccgaagtgca gcgtgcgtcc gccgggcgca
     5101 ccggtgaact cgtggctgct gcggtcggac ggaatgaagc tgttgacccc ggccatcgtg
     5161 gtgttgttcg agccggcgag gtcggccgaa ccgccccaca actcaggcac cggtgcggcc
     5221 agcgccgaca gcaccttgcc ggacgcggcc cgggtggcca tcgagccggg ctcgaagacc
     5281 ggcagagcgg cgtccagatc ggccggcacc tcgcgggcca acacgcggtc gagcagttcg
     5341 gcctgcgtgg ggttcgccga ccgccaggtc tcgtagcggg gctcccactc gtcgtgcagc
     5401 gccttggccc gctcggcggc gttggcccgc acggcgtcca cgatcgaggt gtcgatcgcg
     5461 aacggctcgt tctcgaagcc gagttcggcc ttcagcgcgg cgatctcgtc ggcgccgagc
     5521 ttgctgccgt ggacgctgtg gtcgccggcc ttgttgggca gcggccagcc gatgatcgtg
     5581 gtcaccttga tgaaatgcgg cttgtcggtg acggccttgg cccgctcgag cacgtcgtag
     5641 agctcagcga ccttctcttc gtaggtggtg aagccgttgg tgaagtcgac gtggtaggtg
     5701 tcccagccgt aggcgtcgta gcgggcctgg acgtcttctg agaatgcgat cttggtgtcg
     5761 ccctcgatcg agatgcggtt gtcgtcgtag atcaggaaca ggttgcccaa ctcgagcgtg
     5821 gccgccatcg agctggcctc acccgagacc ccctcctgca tacagccatc gccggcgatc
     5881 gcgtacacgt tgtggtcgaa caccgactcg ccgggggtgg cctgcgggtc gtacagctcg
     5941 cgggagcgcc gggcgctcat cgcgaagccg accgcattgg caacaccggc gcccagcggg
     6001 ccggtggtgc actccacgcc cttggtcagg ccgaactcgg ggtggccggg ggtgagcgaa
     6061 tcccacgtgc gcaggctttg catgtcgttc agttcgagcc cgaacccgtt gaggtagagc
     6121 tgcgcgtact gcgtcatcga cgagtgccca gccgacagca cgaaccggtc gcgacccagc
     6181 caacgcggct cggcgggatc gaccttcatc accttggcgt agagcaggta ggcgaccggc
     6241 gccagcgaga ccgcggtacc ggggtgcccg ttgccgacct tctcgacggc atcggcggcg
     6301 agcacgcggg cgatgtcgac cgccttggcg tcctgctcgg tccaggtgag agtactcatg
     6361 ggcgcttccc ttcgaatgac acgcctcgct gcgtggagac gccggtggaa tcagtcacag
     6421 ccaaccctat taggtgcgtc caggcgctgg ggcgatgggt ggggaagtct cgcattttgc
     6481 gtgagtacgt gggtgcggcc cgcattgtgc gtgtggacgt gggtgcagcg ccgtatcggg
     6541 gcctcggagc cgttcgggaa cgcggcccag cacgaccggc tgatccgccg aacccccacc
     6601 caactatctc ccgcctgcga agcttcgcag gcgggagata gttgggcggg cgttcgggca
     6661 ggacgggccg ccggcttcgg gctcagcttt cgagggcgcg tccggtctgg tcgggcaggg
     6721 ccagcgcggc tccggcggcc aacaggaacg ccaccccgaa gacaccgaac accagcacca
     6781 tgccgccggc cccgagcagc aacggcacgc tcagtggcgc gagcatggac gcgatccgtc
     6841 cgaacgctgc ggccgccccc gtgcccttgc cgcgcaccgc ggtcgggtac aactcggggc
     6901 cgacggcgta cagcgcaccc caggcgccga ggttgaagaa cgacagcgca cacccggtga
     6961 cgatgatcag tgtctcggtg gtggcgaacc cgaatgcggc agctgcgagg gccgagccgg
     7021 ccaggaacgt cgcgagggtg atccggcgcc cccacgtctc gatcagccag gcggcgaccg
     7081 catagcccgg aatctgcgcc aatgtgatga tcagcgtgaa ctcgaagctg cgcaccagcg
     7141 ggaagccgcg ctgcaccagc agggtcggaa tccagatgaa cgccccgtag tagctgaggt
     7201 tgatgcagaa ccagacggcc cacagtgcgg cagtgcgggc ccgataggcc ggcgaccaga
     7261 tcgagtcggt gggcgtagcc ctggccggcg gcgcgtccgg gctgggcacg ggttcgacac
     7321 ctgccgcctc ctcgaaacta cgcacggcgg cctctgcctg gtcgatgcgt cccttcgatt
     7381 ccagaaaccg gacggattcg ggcagccccc aacggatcgc ggcggcatag gccgtgggga
     7441 tgacgccgac ggccagggcc cagcgccacc cgtccgggct ggagggcacc accagatagc
     7501 cgatcagcgc ggcgaggatc cagcccacgg cccagaacgc ctccaacgcc accaccaccc
     7561 gtccgcggat ggccttcggg gcatactcgc tgaccagcgt ggacgccacc ggcaactcgg
     7621 cccccagccc gatgccgacg atgaaccgca gcgcgatcaa catggccacc gaggtgacca
     7681 gggcggatgc gccggtggcg accccgtaga gcaacagcgt gagtgcgaat acctggcggc
     7741 gcccgatccg gtcggcgagc agcccgccca gggacgcccc gatcgccatg ccgacgaagc
     7801 cgatcgagcc gatccacgac agcgtggtcg agtcgagctt ccactgcgcg gccagcgccg
     7861 ccatcacgaa cgagatcagc ccgacgtcca tcgcatccag cgcccagccg aggcccgagc
     7921 cgagcagcag cttgccgtgc ttgcgggtga acggcagtcg gtccaggcgc tctgcccggg
     7981 tgagggacgt ggcgttgctg acgtcggtca ttattggtgc tccttgccaa taaggctgcc
     8041 tcagtttagg gacgcggagt ggccccgaca aggcatactc gaccgatgaa cgcgcaaccg
     8101 accgaccatc gcacagcaac tctgatgggc gccgggctga tcggcatggg ggctttgcac
     8161 ttcgcggcgc cgaaggggtt cgacgcgatc attccgcagg agctgcccgt tgcaccgcgc
     8221 accctcacct acgcctcggg cgtcgccgaa ctggccatcg gggcaggcct gctgcacccc
     8281 gcctcccgca aggtgtcggc actcgcggcg gccgccctgt tcgtggccgt ctaccccgcg
     8341 aacctcaaca ctgtgcgggt ggtggccgac cagccgttgc cgtaccggtt gctcgcatgg
     8401 gcccggctgc cgcttcaggt tccgctgatc gtggccgcct ggcggatctt ccgccgcgcg
     8461 gagtaatgac gcccaggctg aatgcgccca gtcgtgccac cgaaggctga tcgaggcgca
     8521 acccggtcat cgcaggtcga gcgggtcgag cgggtcggag accaccgtga gcgtcggctc
     8581 gcccagctca acacgggtgg cggctcgatc cgcgcatccc cgctcagggc gtgacggcga
     8641 tcttgacccc caggcggtcg cggacggcgt cgtaggcggc cggagcctcg tccaacccga
     8701 aggtgttggt gacgatggcg tcggcgttca ggtgtcccga ttccagaagt cggaccgcgg
     8761 cctgcacgtc gcggcgccgg gcgttgctgc cgccggtgac ctcgagttcc ttgtagtgca
     8821 gcaggttgac gtcgacctcg gcggtcgaac ccttcgggaa accggcgaag tagctgaccc
     8881 ggccccccgg acgggccagg tcgagcgcca ccgaagccag gctcggctca ccgatgcaga
     8941 tcacgacgac atcggcgccc aggccgccgg tggcgtccag cacggcctgc ttgacctcgt
     9001 cggggccgac cgcgaccgct cccaacgccg ccgccggtgc ccgcctggcg accgagcgat
     9061 tcgacacgat cacctgggac gcaccagagg cctgggccaa ttgcaggtgc agcagcccga
     9121 tcgggccggc gcccagaatc gccactacgt cgccgggctg tacccggtac tggttgagcg
     9181 cgttgataca gcacgacagg ggctctgcga gcgcgagatg tgtgggtttc acccgcgtgc
     9241 gcgtggtgac caggttgccc tgggcgaccg ctcgggccgg aatcaggcag tagtcggcca
     9301 gcccgccgtc gatctcgtag ccgatcagct gcaggttctc gcacaggtgc tccagaccca
     9361 gcaggcagac ccggcagtgg ttgcacgaga tcacgatgga cggcgccgcc tgctgcccga
     9421 cctcgtaccc ggtgacgccg tcgccgaggg cggcgacgcg ccccgcgatc tcgtgaccca
     9481 tgatcacgcc ttggcggacg cccgcc
//
LOCUS       DAOSUG010000040         9398 bp    DNA     linear   ENV 10-JUN-2024
DEFINITION  MAG TPA_asm: Micropruina sp. isolate AMR_MDS_2195
            NODE_3936_length_9398_cov_4.347426, whole genome shotgun sequence.
ACCESSION   DAOSUG010000040 DAOSUG010000000
VERSION     DAOSUG010000040.1
DBLINK      BioProject: PRJNA925477
            BioSample: SAMN32864262
            Sequence Read Archive: SRR4236673
KEYWORDS    WGS; ENV; Metagenome Assembled Genome; MAG; Third Party Data; TPA;
            TPA:assembly.
SOURCE      Micropruina sp. (wastewater metagenome)
  ORGANISM  Micropruina sp.
            Bacteria; Bacillati; Actinomycetota; Actinomycetes;
            Propionibacteriales; Nocardioidaceae; Micropruina.
REFERENCE   1  (bases 1 to 9398)
  AUTHORS   Abdulkadir,N., Saraiva,J.P., Zhang,J., Stolte,S., Gillor,O.,
            Harms,H. and Rocha,U.
  TITLE     Genome-centric analyses of 165 metagenomes show that mobile genetic
            elements are crucial for the transmission of antimicrobial
            resistance genes to pathogens in activated sludge and wastewater
  JOURNAL   Microbiol Spectr 12 (3), e0291823 (2024)
   PUBMED   38289113
REFERENCE   2  (bases 1 to 9398)
  AUTHORS   Abdulkadir,N.
  TITLE     Direct Submission
  JOURNAL   Submitted (24-MAR-2023) Environmental Microbiology, Helmholtz
            Center for Environmental Research -UFZ, Permoserstr 15, Leipzig
            04318, Germany
COMMENT     The annotation was added by the NCBI Prokaryotic Genome Annotation
            Pipeline (PGAP). Information about PGAP can be found here:
            https://www.ncbi.nlm.nih.gov/genome/annotation_prok/
            
            ##Genome-Assembly-Data-START##
            Assembly Method        :: SPAdes v. 3.11.1
            Genome Representation  :: Full
            Expected Final Version :: Yes
            Genome Coverage        :: Not Applicable
            Sequencing Technology  :: Illumina MiSeq
            ##Genome-Assembly-Data-END##
            
            ##Genome-Annotation-Data-START##
            Annotation Provider               :: NCBI
            Annotation Date                   :: 05/04/2023 04:57:10
            Annotation Pipeline               :: NCBI Prokaryotic Genome
                                                 Annotation Pipeline (PGAP)
            Annotation Method                 :: Best-placed reference protein
                                                 set; GeneMarkS-2+
            Annotation Software revision      :: 6.5
            Features Annotated                :: Gene; CDS; rRNA; tRNA; ncRNA
            Genes (total)                     :: 2,776
            CDSs (total)                      :: 2,750
            Genes (coding)                    :: 2,714
            CDSs (with protein)               :: 2,714
            Genes (RNA)                       :: 26
            tRNAs                             :: 24
            ncRNAs                            :: 2
            Pseudo Genes (total)              :: 36
            CDSs (without protein)            :: 36
            Pseudo Genes (ambiguous residues) :: 0 of 36
            Pseudo Genes (frameshifted)       :: 2 of 36
            Pseudo Genes (incomplete)         :: 32 of 36
            Pseudo Genes (internal stop)      :: 2 of 36
            ##Genome-Annotation-Data-END##
FEATURES             Location/Qualifiers
     source          1..9398
                     /organism="Micropruina sp."
                     /mol_type="genomic DNA"
                     /submitter_seqid="NODE_3936_length_9398_cov_4.347426"
                     /isolate="AMR_MDS_2195"
                     /isolation_source="Activated sludge of municipal
                     wastewater treatment plants"
                     /db_xref="taxon:2737536"
                     /environmental_sample
                     /geo_loc_name="Canada"
                     /lat_lon="49.25 N 123.1 W"
                     /collection_date="2015-06-16"
                     /metagenome_source="wastewater metagenome"
                     /note="metagenomic"
     gene            complement(<1..167)
                     /locus_tag="PLF56_02365"
     CDS             complement(<1..167)
                     /locus_tag="PLF56_02365"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_019512816.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="MFS transporter"
                     /protein_id="HOQ52438.1"
                     /translation="MALTGLSAVFLFEMLDNSILNVALPTIGRELGASTLELQWVTGA
                     YGVVFGGLMLV"
     gene            274..990
                     /locus_tag="PLF56_02370"
     CDS             274..990
                     /locus_tag="PLF56_02370"
                     /inference="COORDINATES: protein motif:HMM:NF014906.2"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_function: GO:0003677 - DNA binding [Evidence IEA];
                     GO_process: GO:0006355 - regulation of DNA-templated
                     transcription [Evidence IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="TetR/AcrR family transcriptional regulator"
                     /protein_id="HOQ52439.1"
                     /translation="MADTTRALPPRDRWAHRQTDSSGAAAVPKPAITLDRILDAAFAL
                     IRSDGYEALTMRRLASALGTGAASLYAHVRNKADLDDLLIGELCAQIRLPSPDPSAWR
                     EGFVDVCHQLLTQYRRYPGVSRAVLTMAPQSLDTLRISEGLLAILLAGPNAVRDAAWT
                     IDAALLYVSGYALEDSLSGRHAQGPARHHETAQRLATLPPARFPLTLAHASELTSGEG
                     DARFDFTLDLLLDGLAGRET"
     gene            complement(1024..1968)
                     /locus_tag="PLF56_02375"
     CDS             complement(1024..1968)
                     /locus_tag="PLF56_02375"
                     /EC_number="2.7.4.16"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_015070739.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_function: GO:0009030 - thiamine-phosphate kinase
                     activity [Evidence IEA];
                     GO_process: GO:0009228 - thiamine biosynthetic process
                     [Evidence IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="thiamine-phosphate kinase"
                     /protein_id="HOQ52440.1"
                     /translation="MAGRSVAETGEFELIQSITHGLPTSPAVRLGTGDDCAVFSVDGD
                     VAVSTDTMVENVHFRRAWSSPHDVGRKAVASAVSDLEAMGARPVGLVMALTLPPELET
                     AWVSEFGLGVREECASAGLVLLGGDTTRGRDITASATVFGDLQGRAPVTRAGARPGDV
                     VAVCGRLGWAAAGLTVLNRGFRSPRAVVVAHRVPEPPYGQGIVAQQAGATSMIDCSDG
                     LLADLGHVARASGVAIDVHTALLDVAEPQSVVAAAVGGGDPLTFILTGGDDHALLATF
                     APDAVPQGWQVIGQVNAGDPGVTVDGAEWQAEIGWRHF"
     gene            complement(2056..2268)
                     /locus_tag="PLF56_02380"
     CDS             complement(2056..2268)
                     /locus_tag="PLF56_02380"
                     /inference="COORDINATES: ab initio
                     prediction:GeneMarkS-2+"
                     /note="Derived by automated computational analysis using
                     gene prediction method: GeneMarkS-2+."
                     /codon_start=1
                     /transl_table=11
                     /product="hypothetical protein"
                     /protein_id="HOQ52441.1"
                     /translation="MVPISAAAAASVGHVRRVNTEVRSHVHELRRHTSTSSTSTNDQT
                     MRWARISSGVAGFSAARKGGRKPQSR"
     gene            complement(2326..2532)
                     /locus_tag="PLF56_02385"
     CDS             complement(2326..2532)
                     /locus_tag="PLF56_02385"
                     /inference="COORDINATES: ab initio
                     prediction:GeneMarkS-2+"
                     /note="Derived by automated computational analysis using
                     gene prediction method: GeneMarkS-2+."
                     /codon_start=1
                     /transl_table=11
                     /product="hypothetical protein"
                     /protein_id="HOQ52442.1"
                     /translation="MSVDRFFTTPYSPKLQARVSETHSPCQLPAEITSTPSSTMPPPT
                     RAPVPSRSRNSSTPNSTLTRGLMK"
     gene            2646..2906
                     /locus_tag="PLF56_02390"
     CDS             2646..2906
                     /locus_tag="PLF56_02390"
                     /inference="COORDINATES: ab initio
                     prediction:GeneMarkS-2+"
                     /note="Derived by automated computational analysis using
                     gene prediction method: GeneMarkS-2+."
                     /codon_start=1
                     /transl_table=11
                     /product="hypothetical protein"
                     /protein_id="HOQ52443.1"
                     /translation="MLIAAGAVTAIPLWFFAVAAPRLQFGVLGVLQYVAPTIQFLLGI
                     TVFGEHVTVSYWIGLIGVWIGSAIYLAMTIRDREEPAVEEPT"
     gene            complement(2981..4093)
                     /locus_tag="PLF56_02395"
     CDS             complement(2981..4093)
                     /locus_tag="PLF56_02395"
                     /EC_number="6.3.2.-"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_014846408.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_function: GO:0005524 - ATP binding [Evidence IEA];
                     GO_function: GO:0008716 - D-alanine-D-alanine ligase
                     activity [Evidence IEA];
                     GO_function: GO:0046872 - metal ion binding [Evidence
                     IEA];
                     GO_process: GO:0009252 - peptidoglycan biosynthetic
                     process [Evidence IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="D-alanine--D-alanine ligase family protein"
                     /protein_id="HOQ52444.1"
                     /translation="MAQRIRVGIVFGGVSSEHDVSCMTAGGVSRAIDADRFEVVGIGI
                     TPSGRWVQVNPDELRDLRKQGDELPRLSESRPTAVLLPGESGGARIATLQGDRLVDIQ
                     PIDVAFTLLHGPFGEDGTIQGLFEMVGLRYVGSGVAASANGMDKDWMKRTLSASGLPG
                     CRFVTVTARQWSQQRDETLARIEALGYPVFVKPARGGSSVGITRVNSSDELEQAVLMA
                     HKFDPKLVIEEAVLNAREVEFAVLGGRGGAPRVSLPGEIVMRDENAFYDFEAKYVSDD
                     EALLKVPTEMSDELRRRAGDVAARTFEAMGAEGLARVDLFITADGRVLVNEINTMPGF
                     THISMFPSLWAASGIDYTSLITDLIDQAMERPVGLR"
     gene            4558..6264
                     /locus_tag="PLF56_02400"
     CDS             4558..6264
                     /locus_tag="PLF56_02400"
                     /inference="COORDINATES: protein motif:HMM:NF012318.2"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="trypsin-like peptidase domain-containing
                     protein"
                     /protein_id="HOQ52445.1"
                     /translation="MAEPAGTTPQSDADRTGVDAPTEVLPTGVIGPDPTLPMPVSPWS
                     RPDANWAAPDERTRATWAQAGPQQGYAGQQAYPGQPTPGQQAYPGQQGYPSQPYPGQQ
                     GYPSQPYPGQPGFSQQSYAGQGYPVQQGYPQQGSGQEGYPPQAYSGQQNAYPDQQAHP
                     GQDAYPGHPGASYGPPQYPSGSAPGGSLQPAATPPRRGARRGVALVLAVVLALVVAGV
                     GSGWAARVILGTDQPSPSSSQPQIEPSSPVDPAAGGGQPGGSRPTTGGNTTSDEVSAA
                     ESAGVVMIEARSSAGLGAGTGMVLTADGKVLTNYHVVAGSEKLAVTIADSGDTYAATV
                     IGFDQTRDVALLQLKDASGLATVRPDTQVPEVGTNIAAVGNAGGRGALVKAPGNVTGT
                     DQDLTVSSESPWGNTEDLSGLICTDAGAVPGDSGGPMFDDDSEVIGMTTAGSTRKGTS
                     CAVPIGTALQVVAQIDTGRDAGSVRVGPAGYLGIKVDDQQSGTGKTITEVVEGSPAAQ
                     AGMTAGSRLTQVNGTAIRATTNLATVIRALEPGEQVRIEWTTADGTKRSSTVTLGSSP
                     VA"
     gene            6443..6949
                     /locus_tag="PLF56_02405"
     CDS             6443..6949
                     /locus_tag="PLF56_02405"
                     /inference="COORDINATES: ab initio
                     prediction:GeneMarkS-2+"
                     /note="Derived by automated computational analysis using
                     gene prediction method: GeneMarkS-2+."
                     /codon_start=1
                     /transl_table=11
                     /product="hypothetical protein"
                     /protein_id="HOQ52446.1"
                     /translation="MDLTAFYSLMAITCFTLVGLWWTVVERHPESKSDVHSRKLAGGV
                     YLSFLLPGLMSTFAQVDAGNPLVWRSSFTVAAVVGLANTWPLLRLDRNGARGPFRRFR
                     WLVLVIYGAIVVVALVPAVAQAVGATALQSAAIALILLIVLAHGLAWEFMMEPDQPPT
                     SGTTGEPQ"
     gene            7060..8892
                     /gene="recQ"
                     /locus_tag="PLF56_02410"
     CDS             7060..8892
                     /gene="recQ"
                     /locus_tag="PLF56_02410"
                     /EC_number="3.6.4.12"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_019048552.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_function: GO:0003678 - DNA helicase activity [Evidence
                     IEA];
                     GO_process: GO:0006310 - DNA recombination [Evidence IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="DNA helicase RecQ"
                     /protein_id="HOQ52447.1"
                     /translation="MPTPSALSVLQSSFGYDSFRGDQAAIVDHVINGGDALVLMPTGG
                     GKSLCYQIPALVRPGVGVVISPLIALMHDQVTALEANGVRAGFLNSTQSLQQRTATEQ
                     LAMAGELDLLYLAPERLGLPGTQQFLERLEVALFAIDEAHCVSQWGHDFRPDYLKLSV
                     LGERWPQVPRVALTATATPSTRHDVVERLGLARARTFTASFDRPNITYRIEPKQQPVQ
                     QLVQLIRTEHDGQAGIVYCLSRASVEATAAALVAQGITALPYHAGLPAEIRAANQSRF
                     LRNDGVVMVATIAFGMGIDKPDVRFVAHLDLPKSIEGYYQETGRAGRDGLPATAWMAY
                     GLADVVQQRQLIERSEGDAAHKRLQGRLLDAMLGLCETTGCRRQMILDYFGEASGACG
                     NCDTCLNPPDTFDGTIAAQKLLSTVVRLQRERNQRFGAGQLIDILLGRRTEKVQRWQH
                     DQLSTFGLGTEFSDRQWRTVVRQLLAGGVLAVSQDGHSTLTLTESSWPVLKGAQPVLL
                     RSDAVARAGQVKRRVDAGRTKGAVPAELSGQGRELFERLRAWRAETAKEASVPAYVVF
                     PDVTLVAIAQRRPSSLAELSAISGVGAKKLESYGHSVLTLVGDD"
     gene            9166..>9398
                     /locus_tag="PLF56_02415"
     CDS             9166..>9398
                     /locus_tag="PLF56_02415"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_016669267.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="SDR family NAD(P)-dependent oxidoreductase"
                     /protein_id="HOQ52448.1"
                     /translation="MTEPVALVTGGSRGLGLLIATELVERGFHVAISARNADELTRAA
                     DRLGPAARPYTCDVRDRDQVNDLVRRVEADLGPV"
BASE COUNT         1459 a         3326 c         3209 g         1404 t
ORIGIN      
        1 aagaccagca tcagcccgcc gaaaaccacc ccgtaggcgc cggtcaccca ctgcagctcg
       61 agagtggacg cacccagctc gcgtccgatc gtgggcagtg ccacgttgag gatcgagttg
      121 tcgagcatct cgaacaggaa caccgcggac aagccggtca gggcgatcca cgcctctgcg
      181 agcgactgcg gtgaccgccg agcgaacagc gttctattca cgaacactgt tctacacctg
      241 aacactgttc gggtcaagcg gtactgtgct gccatggccg acaccacacg cgcactccca
      301 ccccgagacc gctgggccca ccggcagacc gactcctcgg gcgccgcagc ggtgccaaag
      361 cccgcgatca ccctcgatcg cattctcgac gcggcgttcg ccttgatccg gtccgatggc
      421 tacgaggccc tgaccatgcg ccggctggcc tcggcactgg gcaccggggc agcctcgctg
      481 tatgcgcacg tgcgcaacaa ggccgacctg gacgacctgt tgatcggcga gctgtgcgcc
      541 cagatcaggc tgccgtctcc cgatccgtcg gcgtggcgag agggtttcgt cgatgtctgc
      601 caccagttgc tcacgcagta ccggcgttat ccgggcgtct cgcgcgcggt gctgaccatg
      661 gcgccgcaga gcctggacac gctgcggatc agcgagggcc tgctggcgat tctgctggcc
      721 ggaccgaacg ccgtgcgcga cgccgcctgg acgattgacg cggccctgct ctacgtctcc
      781 ggctacgcgc tcgaggactc attgagcgga cggcacgcgc agggcccggc gcgccaccac
      841 gagacggccc agcggctggc cacgctgccc cccgcacggt ttccgctcac cctcgcgcat
      901 gcgtccgaac tcacctcggg cgagggcgat gcccgcttcg acttcaccct tgacctgctg
      961 ctggacggcc tggccggtcg cgagacctga ggcgcccacg gaggcgcgtc gggtcgtgcc
     1021 cgctcagaag tgccgccacc cgatctcggc ctgccattcg gcgccgtcga cggtgacgcc
     1081 cggatcgccg gcgttgacct ggccgatcac ctgccaaccc tgcggaacag cgtcgggggc
     1141 gaaggtggcg agcagcgcat ggtcgtcgcc gccggtcaga atgaaggtga gtgggtctcc
     1201 cccgccgacc gcggcggcca ccacgctctg gggttcggcc acgtccagaa gtgcggtgtg
     1261 cacgtcgatc gccaccccgg acgccctggc cacgtgaccg aggtcggcaa gcagcccgtc
     1321 cgaacagtcg atcatggacg tggcgccggc ctgttgcgcc acgatgccct gcccgtaggg
     1381 cggttcgggc acccggtgcg cgaccaccac ggcccgtggt gagcggaagc cgcgattcag
     1441 cacggtgagg cccgccgcag cccagccgag gcgtccgcag accgccacca catcgccggg
     1501 acgggccccc gcgcgggtca ccggcgcacg gccctgcaga tcgccgaaga cggtggcgct
     1561 cgcggtgatg tcgcggccgc gggtggtgtc gccgccgagc agcaccagcc cggcgctcgc
     1621 gcactcctca cggaccccca ggccgaactc gctcacccat gctgtttcga gttcgggcgg
     1681 cagcgtgagc gccatgacca gcccgaccgg gcgggcaccc atcgcctcga gatcggacac
     1741 cgccgacgcg accgccttgc gtcccacatc gtgggggctc gaccaggcgc gacgaaagtg
     1801 cacgttctcg accatggtgt cggtgctgac ggccacgtcg ccgtcgacgc tgaagaccgc
     1861 gcagtcgtcg cccgtgccca gccgcacagc gggcgacgtg ggcaacccgt gcgtgatgga
     1921 ctggatcaac tcgaactcgc cggtctcggc caccgatcgt cccgccatga agttgagact
     1981 acggcctgct cggtggaacc atccgatggt gtctccgatc tcgcgcaagc aggccctggg
     2041 cgctgccgtt gcggcctacc tgctctgggg ctttctgccg cccttcctcg cggcgctgaa
     2101 gccggccacc ccgctggaga tcctggccca ccgcatcgtc tggtcgttcg tgctggtgct
     2161 gctggtgctg gtgtgtctgc gcagctcgtg gacgtggctg cgcacctcgg tgttcacccg
     2221 gcggacgtgg cccacgctgg ccgccgcggc ggcgctgatc ggcaccaact ggatggtcta
     2281 catctgggcg gtcaacaaca accacgtggt cgaggcgtcg ctgggttact tcatcaatcc
     2341 cctggtcaac gtgctgttcg gagtgctgct gttccgggag cggctcggca ccggggcccg
     2401 ggtcggcggc ggcatcgtgc tgctcggcgt gctggtgatc tcggccggca actggcaggg
     2461 gctgtgggtc tcgctcaccc tggcctgcag cttcgggctg tacggcgtgg tgaagaaacg
     2521 atcgacgctc acggccctgc aggggctgct ggtggagtcg gccttcctga ccccgctcgc
     2581 gttgccgtac ctgctctggc tgggcccgcg cggccagttc ggcacctctt tgaccatgtc
     2641 ccgtcatgct gatcgccgcg ggcgccgtca cggcgattcc gctgtggttc ttcgccgtgg
     2701 cagctcctcg gctgcagttc ggcgtgctgg gtgtgctgca gtacgtcgca cccacgatcc
     2761 agtttctgct cggcatcacc gtgttcggcg aacacgtcac cgtgtcgtac tggatcggtc
     2821 tgatcggagt ctggatcggc tccgcgatct acctcgccat gaccattcgc gaccgggagg
     2881 agccggccgt cgaagagccc acctgacgag cgtcgggggc cgtccccccg ccgagaccca
     2941 ccgcggagcg cagccccgcc gggccacggt caaccgcaga tcagcgcagg ccgaccggac
     3001 gctccatcgc ctggtcgatc aggtcggtga tcaggctggt gtagtcgatg ccgctggccg
     3061 cccacaacga cgggaacatc gaaatgtggg tgaatccggg catggtgttg atctcgttga
     3121 ccagcacgcg cccgtccgcg gtgatgaaca ggtcgacccg cgccagaccc tcggcaccca
     3181 tcgcctcgaa ggtacgggcg gcgacatcac cggcacggcg acgcagctcg tcgctcatct
     3241 cggtgggcac cttgagcagc gcctcgtcgt ccgagacgta tttcgcttcg aagtcgtaga
     3301 aggcattctc gtcgcgcatc acgatctcac cgggcaggga cacccggggt gcgccaccgc
     3361 gcccgccgag caccgcgaac tccacctctc gggcgttcag caccgcctct tcgatgacca
     3421 gcttggggtc gaacttgtgc gccatcagca cggcctgctc gagttcgtcc gaactgttca
     3481 cccgggtgat ccccacgctg gagccgccgc gcgccggctt gacgaacacc gggtacccca
     3541 gcgcctcgat gcgggccagc gtctcgtcgc gctgctggct ccactggcgc gcggtcacgg
     3601 tgacgaagcg acagcccggc aaccccgagg ccgacaaggt gcgcttcatc cagtccttgt
     3661 ccatgccatt ggcgctcgcc gcgaccccgg agccgacgta gcgaaggccg accatctcga
     3721 acagtccctg aatggtgccg tcttcaccga atgggccgtg caacagggtg aacgcgacat
     3781 cgatcggttg aatgtcgacg agccggtcgc cctgcagggt cgcgatgcgc gcacccccgg
     3841 actcgcccgg cagcagcacc gcggtgggtc gcgactcgct caaccgcggc agctcgtcac
     3901 cctgcttgcg caggtcgcgc agctcatcgg ggttcacctg gacccaacga cccgacggag
     3961 tgatgccgat gcccaccacc tcgaagcgat cggcatcgat ggctcggctg acgccgcccg
     4021 cggtcatgca cgacacgtcg tgctccgagc tgaccccgcc gaagacgatg cccacccgga
     4081 tgcgttgcgc catgggtcct cctgatcgtg gtgcattgtc accctagtgg tgtcggctcg
     4141 cccggacggg ccgcgacacc ggcaccggtg ccatcgcatg gacgggctgg gcaccaggcg
     4201 gtgccagaag gtccccggtc acatgttcgt acgcgcggga tggttccaag gcttcgaact
     4261 gacgatcgtc cgctcgccga catgagcgcg gtgagcgccc gtccgcccca tcagcgccga
     4321 ggcacgacga gtgcgggttc agcacagacc gagacccatc ggttcgcgag tgacggtgtc
     4381 gccggcaggt gtgagccgta gtccgcagaa ctcgaacacc gcggtttcgg gcagccgcgc
     4441 cccggcggac ccgatgaacg gtgactctgc cggccgactc acagggattc atagccaacc
     4501 gacagggtgc gcttggactg atcgccaaca ctggcatgga cgagaggggc aatggccatg
     4561 gctgagccgg cgggaacgac accgcaatcg gacgccgacc gcacgggtgt ggacgcgccc
     4621 acggaggtgc tgcccacggg cgtcatcggg ccggacccca cactgccgat gccggtgtcg
     4681 ccgtggagcc ggcccgacgc gaactgggca gcacccgacg agaggacgcg ggcgacgtgg
     4741 gcccaggccg gtccccagca gggctacgcg gggcagcagg cgtatccggg ccagccgact
     4801 ccgggccagc aggcgtatcc cggccagcag ggctacccgt cgcagcccta tccgggccag
     4861 cagggctacc cgtcgcagcc ctatccgggc cagcccgggt tctcgcagca gagctatgcg
     4921 gggcagggct atccggtgca acagggctac ccccagcagg gatcagggca ggagggttac
     4981 ccgccacagg cctactcggg gcagcagaat gcctacccgg accagcaggc ccacccgggc
     5041 caggatgcct acccgggcca tccgggcgcc tcctacggac cgccgcagta tccgtccggc
     5101 tctgcacccg gcggatccct gcagcccgcg gcaacgccgc cacgccgagg cgcacgccgc
     5161 ggtgtcgcgc tggtgctggc agtggtgctg gcgctggtcg tcgccggcgt gggttccggg
     5221 tgggccgccc gggtcatcct gggcacggac cagccctcgc cctccagcag tcagcctcag
     5281 atcgaaccgt cctcaccagt ggatcccgcg gcgggcggcg ggcagcccgg cgggtcgcgt
     5341 cccaccacgg gcggcaacac caccagcgac gaggtgagcg cggcggagtc cgccggcgtg
     5401 gtaatgatcg aggcgcgctc atcggccgga ctcggcgcgg gcaccggcat ggtgctcacc
     5461 gcggacggca aggtgttgac caactaccac gtggtcgcgg gctctgagaa gctggcggtc
     5521 acgatcgccg attcgggtga cacctacgcg gcaacggtga tcggcttcga ccagaccagg
     5581 gacgtggcgc tcctccaact gaaggacgcc agcggcctgg ccaccgtgcg acccgacacg
     5641 caggtgcccg aggtcggcac gaacatcgcc gcggtcggaa acgccggggg gcgcggtgca
     5701 ctggtcaagg cacccggaaa cgtcacgggc accgaccagg acctgaccgt ctcgtccgag
     5761 tcgccgtggg gcaacaccga ggatctttcc ggcctgatct gcaccgacgc gggtgcggtt
     5821 cccggcgatt ccggcggccc catgttcgac gacgacagcg aagtgatcgg catgaccacc
     5881 gccgggtcca cccgcaaggg caccagctgc gccgttccga tcggaactgc gttgcaggtg
     5941 gtggcacaga tcgacactgg ccgggacgcc ggcagcgtgc gggtcggacc ggccggctac
     6001 ctgggcatca aagtggacga tcagcagtcc ggtaccggaa agacgatcac cgaagtcgtc
     6061 gagggcagcc cggcggcgca ggccggaatg acggccggca gccggctcac ccaggtgaat
     6121 ggaaccgcga tccgcgccac caccaatctc gccacggtga tccgggcact ggagcccggc
     6181 gagcaggttc ggatcgaatg gacgaccgcc gacggcacga agcgttcgag cacggtcacg
     6241 ctgggcagca gcccggtggc ctagggcgcg aaccgcaccc aatacagtgc gtgcgcggct
     6301 aacacctcgg ccgcacagac tgcggccacc gaacctgcgg ttgcccaccc tacgaagcca
     6361 cgacgatgcc ggctgctcca gcggtgccga tggcccgatg gggggtggac tccgcccgat
     6421 cgtcgtgtga cctgtggtga ccatggacct gaccgctttc tactcactga tggcgatcac
     6481 ctgcttcacc ctggtcgggt tgtggtggac tgtggtcgag cgccatccgg agtcgaagtc
     6541 cgacgtgcac agccgcaagc tggccggcgg ggtgtacctc tcgttcctgc tgccggggct
     6601 gatgagcacg ttcgcgcagg tggacgccgg caaccccctg gtgtggcggt cgagcttcac
     6661 ggtggccgcg gtcgtcggac tggccaacac ctggccgttg ctgaggctcg atcgcaacgg
     6721 cgcccgggga ccgtttcggc gcttccgctg gctcgtgctg gtgatctacg gcgcgatcgt
     6781 ggtggtcgcg ctggttccgg ccgtcgccca ggccgtcggc gccacggcgc tgcagtcggc
     6841 ggcgatcgcc ctgatcctgc tgatcgtgct ggcccacggc ctggcctggg agttcatgat
     6901 ggaaccggat cagccgccca cgagcggaac gaccggcgag ccgcagtaga actgcccggc
     6961 accaccacga ggcccgcagg ccgacccggg catcccacgt ccggcgatct gtggcggcgg
     7021 gcgactcgga ctgtctccgg cggcctctac gatggccgcg tgcccacccc gtccgctttg
     7081 agcgtgctgc aaagttcgtt cggctatgac agtttcaggg gcgaccaggc cgccatcgtc
     7141 gaccatgtga tcaacggcgg cgacgcgctg gtgctgatgc ccaccggtgg cggcaagtcg
     7201 ttgtgctacc agattcctgc tctggtgcgg cccggggtgg gggtggtgat ctcgccgctg
     7261 atcgcactca tgcacgatca ggtgaccgcg ctggaggcca acggcgtccg ggccgggttt
     7321 ctgaactcga cgcagtcgct ccagcagcgc acggccaccg agcaactggc gatggccggc
     7381 gagctcgacc tgctgtatct ggctcccgaa cgactgggcc tgccgggtac ccagcagttc
     7441 ctggagcggc tcgaggtggc gctgttcgcc atcgacgagg cgcactgtgt gtcgcagtgg
     7501 ggccacgatt tccggcccga ctacctcaaa ctgtcggtgt tgggcgagcg ctggccgcag
     7561 gtaccgcggg tggcactgac ggccaccgca accccgtcca cacgccacga cgtcgtggaa
     7621 cggctgggcc tggcccgggc ccgcaccttc acggcgagct tcgaccggcc caacatcacc
     7681 taccgcatcg aacccaaaca gcagccggtg cagcaactgg tgcagctgat ccgcaccgag
     7741 cacgacggcc aggccggcat cgtctactgc ctgtcgcggg cgtcggtgga ggccacggcc
     7801 gcggccctgg tcgcccaggg catcacggcc ctgccgtacc acgcggggtt gccggccgag
     7861 atccgggcag ccaaccagtc gcggttcctg cgtaacgacg gcgtggtgat ggtcgccacc
     7921 atcgccttcg gcatgggcat cgacaagccc gacgtccgct tcgtcgcgca tctcgacctg
     7981 cccaagagca tcgagggcta ctaccaagag accggacgcg ccggacgcga cggtctgccg
     8041 gccaccgcat ggatggcgta cggtcttgcc gacgtggtgc agcaacggca actgattgaa
     8101 cgatccgagg gtgacgccgc gcacaagcgg ctgcaggggc gactgctgga cgccatgctc
     8161 gggctgtgcg aaaccaccgg ctgccgccgg caaatgatcc tggactactt cggtgaggcg
     8221 agcggggcct gcggcaactg cgacacctgc ctcaatccgc cggacacctt cgacggcacg
     8281 atcgcggcgc agaagctgct gtccaccgtc gtgcgactgc agcgcgagcg caaccagcgg
     8341 ttcggcgccg gccagttgat cgacatcctg ctcggccggc gcaccgaaaa ggtgcagcgc
     8401 tggcagcacg accagctgag cacgttcggg ctcggcaccg agttcagcga ccggcaatgg
     8461 cgcaccgttg tgcgtcaact cctggcgggc ggcgtgctgg cggtctccca ggacggccac
     8521 agcaccctca ccctcaccga gtcgtcgtgg ccggtgctga agggtgccca gccggtgctg
     8581 ctgcgctcgg acgccgtcgc gcgcgccggg caggtcaagc gacgcgtcga cgcgggccga
     8641 acgaagggcg cggttccggc cgagctgagc ggccagggtc gagaactgtt cgagcggttg
     8701 cgggcctggc gcgccgagac ggcgaaggag gcgtccgtgc cggcctatgt ggtgttcccc
     8761 gacgtcacgc tggtggccat cgcgcagcgg cgtccgagct cacttgcgga actgagcgcg
     8821 atcagcggcg tgggcgcgaa gaagctggag tcgtatgggc actctgtgct cacgctggtc
     8881 ggtgacgact gatcgtcggc ggtccccgtg ggggcgagat cgacgccggc gatcgcaggg
     8941 agcacccgaa cggcacgagt ggcgcatgac ccccaccagg cccgtcgcgc tcaccggggc
     9001 cgccggttgc ctcggaggcc ggatcgcgca ccggcacgcc accgccgcgt accgcatgat
     9061 ccagcagcag aagcaccggc gcctcgcggt gatgctgcac ccctggccgg taggagaccc
     9121 tacgggaggt agtccggcgc ggatgagact ggctagcgtg gcgggatgac cgaacccgtt
     9181 gccctcgtca ccggcggctc ccgcggcctc ggcctgctca tcgccaccga actcgtcgag
     9241 cgcggattcc acgtggccat ctctgcgcgc aacgccgacg aactcaccag ggcggccgac
     9301 cggctcgggc ctgccgctcg cccgtacacc tgcgacgtgc gtgaccgcga ccaggtgaac
     9361 gacctggtgc gtcgcgtcga ggccgacctc ggccccgt
//
LOCUS       DAOSUG010000041         9280 bp    DNA     linear   ENV 10-JUN-2024
DEFINITION  MAG TPA_asm: Micropruina sp. isolate AMR_MDS_2195
            NODE_3981_length_9280_cov_4.975718, whole genome shotgun sequence.
ACCESSION   DAOSUG010000041 DAOSUG010000000
VERSION     DAOSUG010000041.1
DBLINK      BioProject: PRJNA925477
            BioSample: SAMN32864262
            Sequence Read Archive: SRR4236673
KEYWORDS    WGS; ENV; Metagenome Assembled Genome; MAG; Third Party Data; TPA;
            TPA:assembly.
SOURCE      Micropruina sp. (wastewater metagenome)
  ORGANISM  Micropruina sp.
            Bacteria; Bacillati; Actinomycetota; Actinomycetes;
            Propionibacteriales; Nocardioidaceae; Micropruina.
REFERENCE   1  (bases 1 to 9280)
  AUTHORS   Abdulkadir,N., Saraiva,J.P., Zhang,J., Stolte,S., Gillor,O.,
            Harms,H. and Rocha,U.
  TITLE     Genome-centric analyses of 165 metagenomes show that mobile genetic
            elements are crucial for the transmission of antimicrobial
            resistance genes to pathogens in activated sludge and wastewater
  JOURNAL   Microbiol Spectr 12 (3), e0291823 (2024)
   PUBMED   38289113
REFERENCE   2  (bases 1 to 9280)
  AUTHORS   Abdulkadir,N.
  TITLE     Direct Submission
  JOURNAL   Submitted (24-MAR-2023) Environmental Microbiology, Helmholtz
            Center for Environmental Research -UFZ, Permoserstr 15, Leipzig
            04318, Germany
COMMENT     The annotation was added by the NCBI Prokaryotic Genome Annotation
            Pipeline (PGAP). Information about PGAP can be found here:
            https://www.ncbi.nlm.nih.gov/genome/annotation_prok/
            
            ##Genome-Assembly-Data-START##
            Assembly Method        :: SPAdes v. 3.11.1
            Genome Representation  :: Full
            Expected Final Version :: Yes
            Genome Coverage        :: Not Applicable
            Sequencing Technology  :: Illumina MiSeq
            ##Genome-Assembly-Data-END##
            
            ##Genome-Annotation-Data-START##
            Annotation Provider               :: NCBI
            Annotation Date                   :: 05/04/2023 04:57:10
            Annotation Pipeline               :: NCBI Prokaryotic Genome
                                                 Annotation Pipeline (PGAP)
            Annotation Method                 :: Best-placed reference protein
                                                 set; GeneMarkS-2+
            Annotation Software revision      :: 6.5
            Features Annotated                :: Gene; CDS; rRNA; tRNA; ncRNA
            Genes (total)                     :: 2,776
            CDSs (total)                      :: 2,750
            Genes (coding)                    :: 2,714
            CDSs (with protein)               :: 2,714
            Genes (RNA)                       :: 26
            tRNAs                             :: 24
            ncRNAs                            :: 2
            Pseudo Genes (total)              :: 36
            CDSs (without protein)            :: 36
            Pseudo Genes (ambiguous residues) :: 0 of 36
            Pseudo Genes (frameshifted)       :: 2 of 36
            Pseudo Genes (incomplete)         :: 32 of 36
            Pseudo Genes (internal stop)      :: 2 of 36
            ##Genome-Annotation-Data-END##
FEATURES             Location/Qualifiers
     source          1..9280
                     /organism="Micropruina sp."
                     /mol_type="genomic DNA"
                     /submitter_seqid="NODE_3981_length_9280_cov_4.975718"
                     /isolate="AMR_MDS_2195"
                     /isolation_source="Activated sludge of municipal
                     wastewater treatment plants"
                     /db_xref="taxon:2737536"
                     /environmental_sample
                     /geo_loc_name="Canada"
                     /lat_lon="49.25 N 123.1 W"
                     /collection_date="2015-06-16"
                     /metagenome_source="wastewater metagenome"
                     /note="metagenomic"
     gene            <1..812
                     /locus_tag="PLF56_02420"
     CDS             <1..812
                     /locus_tag="PLF56_02420"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_007077463.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=3
                     /transl_table=11
                     /product="transglutaminase family protein"
                     /protein_id="HOQ52449.1"
                     /translation="RPYRGRLIRWGTRLHDRFLLPAFAAADLREVVGDINEWLAQAAP
                     GSRFDPAWLDPFFEFRFPRLGESEVAGVRLELRQAIEPWHVLGEEATATGTARYVDSS
                     VERVQVAASGLIEGRHVVTCNGVPVPLTAVEHGGWGTALGSDDAPASTGMFVGGVRYR
                     AWAPWSALHPTMGVHAPLVFDLVDTWNDRSLGGFTYHVVHPGGRNYDTYPVNAVEAES
                     RRASRFSEQGHTPGPIDTAGWPAAEAVFGPMGTEYPCTLDLRRYSPGHVNN"
     gene            847..3357
                     /locus_tag="PLF56_02425"
     CDS             847..3357
                     /locus_tag="PLF56_02425"
                     /inference="COORDINATES: protein motif:HMM:NF025761.2"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="circularly permuted type 2 ATP-grasp protein"
                     /protein_id="HOQ52450.1"
                     /translation="MSAAVSLDTGVLDAYRARLGAGAVDELVTDSGLRADQAALGAAV
                     DAMGLGGLLTARAEARTFAADEGVTYGTERSGSRSWAIDPIPVVLGAAEWTTLEAGLR
                     QRATLLDLVLSDLYGERTLLRRRVIPPQLVLAHDGYIRQTDGIGAPGRRQLVVAAADV
                     GRNADGVWTVISDRTQAPSGPGYAMATRRIISRVMAGLHRGTALARLRGFFQTFTASL
                     QDASPSATEPPRVVLLTPGPGSETAFEQAYLATLLGFPLVEADDLVMQRGMVWLRAGD
                     RLEQVDVILRRVDAAYSDPLELRGDSQLGIPGLIEAARTRTVAVVNPIGAGVLENPGL
                     VPHLHAAARALLGEDLLLPMPATWWCGEASARSHVLANLQRLVIKPISRGGNLLRYGW
                     QLSETERADLAAQISAEPWAWCGQEPMQLSTAPVVTRTGLQPRRFVLRTFGVAHDDDY
                     HFLPGGLGRVAPAEDQYTVSNFSGALAKDVWVISSGSDQPSVGRDRRLHQPPIDRAAG
                     LSPRVADNLYWMGRYAERAEGTARLLKVVDDFAEDNSSRTGTPGAQATEVLLDAVAWV
                     TVTGSIREGETSLAYLRRAVLDAARTGTVRHCANQVIKAAQHVRDLLSVDTWSVIGRL
                     QQTLATTPDDNDQLQPLLADVLESLLALDGILAQSMVRDESWAFLDAGCRMERAQMTL
                     SLLGRTLTGERDRAVNDLVVEGVLRAGESIITHRRRAAAGTGPTMALESAVDLLLLDR
                     QNPRSVLYQAQCLAGDLTLLGDEASAARALALAQQIADSDVDELLRPSVLSVTLASLE
                     QDFRRLSSDMAARHFVRQAPRRAQTSGWGSQWQVTDGR"
     gene            3347..4276
                     /locus_tag="PLF56_02430"
     CDS             3347..4276
                     /locus_tag="PLF56_02430"
                     /inference="COORDINATES: protein motif:HMM:NF013961.2"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="transglutaminase family protein"
                     /protein_id="HOQ52451.1"
                     /translation="MAADSRLDHYAPRRYRVRHSTIYTYDDEVEACYERGFLRPLETW
                     SQQVVANEVAVSPEPEVISQHVDHFGNHSYYVEIRSPHRELVVSKTAFVDVAWPRPVL
                     DSLNDFTVAQAAERVEQEADPVQHAEYLLPSDLVELGPEVLAYARSILASERPLGEAL
                     VGLYSTIYRDFKYELGATSVRTTLPELLRLRKGVCQDFAHLAVGCLRSVGLPARYVSG
                     YVETSPPPGQPKLEGSDASHAWTSVLVPGGEWIDLDPTNDHFADSRYIVTAWGRDFRD
                     VSPLKGVVYTESKKSTLEVKVDVFRVNPDGTLS"
     gene            complement(4415..5281)
                     /locus_tag="PLF56_02435"
     CDS             complement(4415..5281)
                     /locus_tag="PLF56_02435"
                     /inference="COORDINATES: protein motif:HMM:NF016370.2"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="DUF559 domain-containing protein"
                     /protein_id="HOQ52452.1"
                     /translation="MNAAQELLRANEGVLCQRLHPRGTLNGLLTRHAIVRVLPGVFVD
                     TELLACRRTRYLAALTARPAAVLWGDSAVAAITADRTPFVAGELIELAEPEGRRIAGL
                     TVRRRSAHSIRLFNGLRCPTPAIVAVEAAVRDQGRTAELLLRTGLVAPAQLVDALSLF
                     DGSCGQQVRRDVIVSYADNPWSGGERDLQQVLRGAGVHGWVANVAIRLAGATYYPDLL
                     FEAARLIVEFDGFAVHGTRQAFEADRVRQNRLMAAGFRVLRYTWQRLQDDPEGIVAEI
                     KSLLAQSEPTIC"
     gene            5370..6293
                     /gene="ligD"
                     /locus_tag="PLF56_02440"
     CDS             5370..6293
                     /gene="ligD"
                     /locus_tag="PLF56_02440"
                     /EC_number="6.5.1.1"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_019045792.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="non-homologous end-joining DNA ligase"
                     /protein_id="HOQ52453.1"
                     /translation="MASPAIEIEVGERTVRVTNPDRTYFPDTGWTKRDLIDYYLSVGP
                     GIVRALRERPCMLHRFPDGLAGPKVHQKRLPGGAPDWVQTVELWFPRYGRTADELCVT
                     ELAQVIWAVQMSTVEFHPWNSRRGHVEQPDEWRIDLDPMPEADFDTVRRVAGVAREVL
                     AELDVTGFPKTSGGKGFHIYVRIEPAWGFAEVRRAALAFAREVERRCADATTVWWRKD
                     RDPATVFVDYNQNARDHTIACAYSVRGTPTGTVSTPVTWAELDDLDPRDFTMASVPAR
                     FAALGDLHAGIDEQSFRLDTLLAWADRDGLD"
     gene            complement(6379..7128)
                     /locus_tag="PLF56_02445"
     CDS             complement(6379..7128)
                     /locus_tag="PLF56_02445"
                     /EC_number="3.5.1.-"
                     /inference="COORDINATES: protein motif:HMM:NF014625.2"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_function: GO:0019213 - deacetylase activity [Evidence
                     IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="PIG-L family deacetylase"
                     /protein_id="HOQ52454.1"
                     /translation="MTEAPSIEPLPDEDFRRVLCVVAHPDDLEYGASAAVAEWTDRGV
                     EVAYLLLTQGEAGMQIPPQECARIRRGEQERACEQVGVSNLTMLAHPDGMLVYSLELR
                     RDIARVIRQFKPDAVLTMTWDIEAPWGLNMADHRVAGLVTIDAIRDADNTWVHPELAK
                     DENLAKWGTRWLLVGGHQQPSHGVAVSDASVQRAVKSLEQHEEYLAAIPEHPKPAEFI
                     PEMLAGGGKAMGVEHAFVVKAYDLRGRPAGA"
     gene            complement(7232..7669)
                     /locus_tag="PLF56_02450"
     CDS             complement(7232..7669)
                     /locus_tag="PLF56_02450"
                     /inference="COORDINATES: ab initio
                     prediction:GeneMarkS-2+"
                     /note="Derived by automated computational analysis using
                     gene prediction method: GeneMarkS-2+."
                     /codon_start=1
                     /transl_table=11
                     /product="hypothetical protein"
                     /protein_id="HOQ52455.1"
                     /translation="MTRSDRVECAVIDSEPAASWHTAYQRSFRNTIGQTAPLTCSEPP
                     SSSLPATYPGLTDEFTTWVFTNVEASAGVELTYRWSAKGGSVEPVAVPSGWKYSCEFG
                     TSTYSVHGHTTRYWSNGGVTRTDWTFMAPADGQRWQVFKDRLG"
     gene            complement(7916..>9280)
                     /locus_tag="PLF56_02455"
     CDS             complement(7916..>9280)
                     /locus_tag="PLF56_02455"
                     /inference="COORDINATES: protein motif:HMM:NF017964.2"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="amylo-alpha-1,6-glucosidase"
                     /protein_id="HOQ52456.1"
                     /translation="STGDSVHLCWQCTVPAYGSVTVGWQLAARDDRLPFVAAQSEPLQ
                     VPTLVDASASLQRLVQRSIADLNALRLAERDHPSHTFFAAGAPWFLTLFGRDSLIAAR
                     LLLPVDSGPALGTLRTLAARQGRGVDIDRAEQVGKILHEVRAEALDLQQGIVLPPEYY
                     GTIDATALWIVLLGELADTGIDPRAEGLFEPLEAALGWLRDSSDADGDGFAEYFDTSG
                     HGLSNQGWKDSGDSVRFADGTIAEGPIALAEVQGYAYAAALAGADLLERFGTDPDAAT
                     GRRDWAQQPADRFRQRFWIADERGAYPAMALDRHKRPVTGVASNMGHLLGTGLLNPAE
                     EQTVVRRLMGQDMFSGYGVRTMSTTNAAYWPTRYHVGSVWTHDTAMIIEGMLRAGYPD
                     EAASLARGLLRAAEGFDHRLPELFGGQSADKVFPPVPYPASCRPQAWAAAAGISVARA
                     LGAL"
BASE COUNT         1428 a         3243 c         3162 g         1447 t
ORIGIN      
        1 accgtcccta ccggggccgg ctgatccgtt ggggcacccg gttgcacgac cgctttctgc
       61 tgccggcctt cgccgccgcc gacctgcgcg aggtggtcgg cgacatcaac gagtggctgg
      121 cgcaggccgc cccgggcagc cgcttcgacc cggcctggct cgacccgttc ttcgagttcc
      181 gctttccgcg gctcggcgag agcgaggtgg cgggcgtccg gctggagttg cggcaggcta
      241 tcgagccctg gcacgtgctg ggtgaggagg ccacggccac cggcacggcc cgctacgtcg
      301 actcgtccgt cgaacgagtg caagtggcgg cttccggtct gatcgagggc cggcacgtgg
      361 tgacctgcaa cggggttccg gtgccgctga ccgcggtcga gcacggcggt tggggcactg
      421 ccctgggctc ggacgacgcg cccgccagca ccggcatgtt cgtcggcggc gtgcggtacc
      481 gggcctgggc cccctggtcg gccctgcacc ccaccatggg cgtgcacgca ccactggtgt
      541 tcgacctggt cgacacctgg aacgaccgct cgctgggcgg cttcacctac cacgtggtgc
      601 atcccggagg tcgcaactac gacacctacc cggtcaatgc ggtcgaggcc gagtcgcgcc
      661 gggcgtcgcg gttcagcgag caggggcata ctcccgggcc gatcgacacc gccggctggc
      721 cggcggccga ggcggtgttc ggcccgatgg gcaccgaata cccctgcacg ctcgacctgc
      781 gtcgctactc gcccggccac gtgaacaact gaacagcagg acagctctac cccttaaggt
      841 ggcgccatgt ccgctgcggt aagcctcgat accggtgtgc tcgatgccta tcgagcccgt
      901 ctgggcgcgg gcgcggtcga cgaactggtc accgactcgg ggttgcgcgc cgaccaggcc
      961 gccctcggtg ccgccgtgga cgcgatgggg ctgggcggcc tgctgacggc ccgggccgag
     1021 gcccgcacct tcgctgccga tgaaggcgtc acctatggca ccgaacgcag cggcagccgc
     1081 agttgggcga tcgacccgat cccggtcgtg ctgggcgccg ccgaatggac gacgctcgag
     1141 gccggcctgc ggcagcgtgc gacgctgctc gacctggtgc tctcggatct gtacggcgag
     1201 cgcacgctgc tgcggcggcg ggtgattccg cctcagctgg tgctggccca cgacggctac
     1261 attcgccaga ccgacggcat cggcgctcct ggacgacgcc aactggtggt ggccgcggcc
     1321 gatgtcggac gcaacgccga cggcgtctgg acggtgatct cggaccgcac ccaagccccg
     1381 tccggccccg ggtatgcgat ggcgacccgg cggatcatct cgcgggtgat ggccggcctg
     1441 caccgtggca ctgcgctggc ccggctgcgc ggcttcttcc agaccttcac cgcgtcactg
     1501 caggacgcgt cgccgtcggc caccgagccg ccgcgggtgg tgctgctcac cccgggtccg
     1561 ggcagtgaga ccgccttcga gcaggcgtac ctggccaccc tgctgggttt cccactggtc
     1621 gaggccgacg acctggtgat gcagcggggc atggtgtggt tgcgcgccgg tgaccggctc
     1681 gaacaggtcg acgtgattct gcgccgggtg gacgctgcct attccgaccc cctcgaactg
     1741 cgcggcgatt cccagctggg cattcccggg ttgatcgagg cggcgcgcac ccgcacggtc
     1801 gccgtggtca atccgatcgg cgccggggtg ctcgagaacc ccggcctggt tccgcacctg
     1861 cacgcagcgg cccgcgccct actgggcgaa gacctgctgc tgccgatgcc cgccacctgg
     1921 tggtgcggcg aggcgtccgc ccgttcgcat gtgctggcca atcttcagcg cttggtgatc
     1981 aagccgatct cgcgcggcgg caatctgctg cggtacggct ggcagttgtc cgaaaccgag
     2041 cgtgccgacc tcgcggccca gatcagcgcc gaaccgtggg cctggtgcgg gcaggaaccg
     2101 atgcaactgt ccaccgcccc agtggtcacg cgcaccgggc tgcagccacg tcgcttcgtg
     2161 ctgcgcacct tcggcgtcgc ccacgacgac gactaccatt ttctgcccgg cggtttgggc
     2221 cgggtggccc cggccgagga ccagtacacc gtctcgaact tcagcggcgc cctggccaaa
     2281 gacgtctggg tgatcagctc cgggtccgac cagcccagcg tgggtcgcga tcgccggctg
     2341 catcagcccc cgatcgaccg cgccgccggc ctgtcgccgc gcgtggccga caacctgtac
     2401 tggatgggtc gctacgccga acgcgccgag ggcaccgccc ggctgctcaa ggtggtcgac
     2461 gacttcgccg aagacaacag cagccgcacc ggcacgcccg gtgctcaagc aaccgaggtg
     2521 ttgctggacg ccgtcgcctg ggtcacggtg accggctcca tccgcgaggg cgaaacgtcg
     2581 ctggcctacc tgcgtcgtgc cgtcctggac gcggcgcgca ccggtaccgt tcggcactgc
     2641 gccaaccagg tgatcaaggc cgcacagcac gtgcgcgacc tgctcagcgt cgatacctgg
     2701 tcggtgatcg gacgcctgca gcagacgctg gccaccaccc ccgacgacaa cgaccaactg
     2761 cagccgctgc tggccgacgt gctcgaatcg ctgctggcac tcgacggcat tctggcccag
     2821 agcatggtgc gcgacgagtc gtgggccttt ctcgacgccg gctgtcgcat ggagcgggcc
     2881 cagatgacgc tgtcgttgct gggccgcacc ctgaccggcg aacgcgatcg cgcggtgaac
     2941 gacctggtgg tcgagggcgt gctacgggcc ggagagtcga tcatcacgca ccgtcgccgt
     3001 gctgccgccg gcaccggccc gaccatggcg ctggaatcgg ccgtcgacct gctgctgctc
     3061 gatcgtcaga acccgcgttc ggtgctctac caggcccaat gcctggccgg cgacctgacc
     3121 ctgctgggcg acgaagcgtc cgctgcccgg gcgctggcgc tggcgcaaca gatcgccgac
     3181 tccgatgtgg atgagttgct acgcccgtcg gtgttgtcgg tgaccctcgc gtcgctggag
     3241 caggacttcc gccggctctc cagcgacatg gcggcgcggc acttcgtacg gcaggctccg
     3301 cgccgggcgc agacgtccgg ctggggcagc caatggcagg tgaccgatgg ccgctgattc
     3361 gcgcctggac cactacgcac cccgccgata ccgggtgcgg cactccacga tctacaccta
     3421 cgacgacgag gtcgaggcgt gttacgaacg cggcttcctg cgtccgctcg agacgtggtc
     3481 gcagcaggtg gtggccaacg aggtggcggt gtctcccgaa cccgaggtga tcagccagca
     3541 cgtcgaccac ttcggcaacc atagctacta cgtcgagatc cgctcgccgc accgtgagct
     3601 ggtggtcagc aagaccgcgt tcgtcgacgt ggcctggcca cgcccggtgc tcgactcgct
     3661 gaacgacttc acggtggcgc aggcggccga gcgggtcgag caggaggccg atcccgtgca
     3721 gcacgcggag tacctgctgc cgtccgacct ggtcgaactg gggccggagg tgctcgccta
     3781 cgcacgatcg atcctggcct cggagcgtcc gttgggggag gcgctggtcg gcctctactc
     3841 gaccatctac cgcgacttca agtacgagtt gggcgccacc tcggtgcgca ccacgctgcc
     3901 ggagttgctg cggcttcgca aaggtgtctg ccaggacttc gcacacctgg cggtcggttg
     3961 cctgcgatcg gtgggcctgc cggcgcggta cgtgtcgggg tacgtcgaaa cctcaccccc
     4021 acccgggcag ccaaagctcg aggggtcgga cgcctcgcat gcctggacct cggtgctggt
     4081 gcccggcggt gagtggatcg acctcgaccc gaccaacgac cacttcgccg attcgcgcta
     4141 catcgtgacc gcctgggggc gggacttccg agacgtgtcg ccgctgaagg gcgtggtcta
     4201 caccgaatcg aagaagtcga cgctcgaggt gaaggtggat gtgttccggg tgaacccgga
     4261 cggcacgctg agttgatctg cgacacaacc ggcgtcacaa aagatttcac tgtgcgcggg
     4321 tataccgatt ttaccgccag tttctctcag aagcggtttg caacgtttca tccgaaaact
     4381 ggcggtaaaa tcagtatatc ggctggtgat gatttcagca gatcgtcggt tcgctctgcg
     4441 caagcaggct cttgatctcg gcgacgatgc cctcggggtc gtcctgtagg cgctgccagg
     4501 tgtagcgaag cacccgaaac ccggcagcca tcaggcggtt ctggcgtacg cggtcggcct
     4561 cgaacgcctg acgggtgcca tgaacggcga acccgtcgaa ctcgacgatc aaccgggcgg
     4621 cctcgaacaa cagatccggg tagtaggtgg cacccgcaag cctgatcgcc acattggcga
     4681 cccacccgtg cacccccgcg ccacgcagca cctgctgcag gtctcgctca ccacccgacc
     4741 aggggttgtc ggcgtagctg actatcacgt cgcgccgtac ctgctggccg caggagccat
     4801 caaacaacga aagcgcgtcg accagctgtg cgggagcgac caacccggtg cgtagcagca
     4861 gttcggcggt tcggccctga tcgcgcaccg cggcctccac cgcgacgatc gccggggtcg
     4921 gacaacgaag gccgttgaac aacctgatgc tgtgagctga gcgtcggcga actgtgaggc
     4981 cggcgattct gcgcccttcc ggctccgcca gctcgatcaa ctcaccggcg acgaagggcg
     5041 tccggtcggc ggtgatggcc gccacggcgg aatcccccca caacaccgcc gccggccgag
     5101 cggtcaaggc ggccaggtaa cgcgtacgcc ggcaggcgag cagctcggta tcgacgaaga
     5161 cccccggcag cacccgcaca atggcatggc gagtcaacag gccattgagc gtgccgcgcg
     5221 gatgcaggcg ctggcagagc acaccctcgt tggcccgcaa caactcctgt gccgcattca
     5281 cgcccctatc cagccacggc tggagcgccg atccggccgt acggccggga atcgggcaaa
     5341 tctgtggata accaactacc gtcagcaata tggcgagtcc agcgatcgag atcgaggtag
     5401 gtgagcgaac ggtccgggtg accaaccccg atcgcaccta ctttcccgac accggctgga
     5461 ccaagcgcga cctcatcgac tactacctgt cggtggggcc gggcatcgtc cgggcgctgc
     5521 gcgagcgtcc gtgcatgctg catcgttttc cggacgggct ggccggcccc aaggtgcatc
     5581 agaagcggct gccgggcggt gcccccgact gggtgcagac cgtcgagctc tggttccccc
     5641 gctacggacg caccgccgat gagctgtgcg tcaccgagct cgcccaggtg atctgggcgg
     5701 tgcagatgtc gacggtcgag tttcacccct ggaacagccg gcgcggccac gtcgagcagc
     5761 cggacgaatg gcgcatcgac ctggacccga tgcccgaggc cgacttcgac acggtgcgac
     5821 gggtggccgg cgtggcgcgc gaggtgctgg ccgaactcga tgtcacgggc tttcccaaaa
     5881 cctcaggcgg caaggggttt cacatctacg tgaggatcga accggcctgg ggtttcgccg
     5941 aggtgcggcg ggcggcgctg gcgttcgcac gcgaggtcga gcgacggtgc gccgacgcca
     6001 ccacggtgtg gtggcgcaag gatcgtgacc cggccacggt gttcgtcgac tacaaccaga
     6061 acgcccgcga ccacaccatc gcctgcgcct attcggtgcg cggcacaccc accggcaccg
     6121 tctcgactcc ggtgacctgg gccgaactgg acgacctcga cccgcgcgac ttcaccatgg
     6181 cgtcagtacc ggcccggttc gccgccctgg gcgacctgca cgccggcatc gacgagcaat
     6241 cgttccggct cgacaccctg ctggcctggg ccgatcgtga cggcctcgac tgacccgggt
     6301 tccccaaccc ccgcctaacc atcgtgcgcc tgcgaagctt cgcaggcgca cgatggtgag
     6361 gcgggggttc ggcgcgggtc aggcgcctgc cgggcgtccg cgcaggtcgt aggccttcac
     6421 cacgaatgcg tgctcgacac ccatcgcctt gccacccccg gccagcatct cgggaatgaa
     6481 ctcggccggc ttgggatgct ccggaatagc tgccaggtac tcctcgtgct gctccagcga
     6541 cttcaccgcc cgctgcaccg aggcgtccga gacggccacg ccgtgggagg gctgctggtg
     6601 cccaccgacc agcaaccagc gggtgcccca cttcgccaga ttctcatcct tggcgagttc
     6661 agggtgcacc caggtgttgt cggcgtcgcg gatcgcgtcg atggtgacca gcccggccac
     6721 ccggtggtcg gccatgttga gcccccaggg ggcttcgatg tcccaggtca tggtcagcac
     6781 ggcatcgggt ttgaactgcc ggatcacccg ggcgatatcg cgccgcagct ccagtgagta
     6841 gaccagcatg ccgtcggggt gggcgagcat cgtcaggttg ctcacgccga cctgttcgca
     6901 ggcgcgttcc tgctcgccgc gccggatgcg ggcgcactcc tgcggcggaa tctgcatgcc
     6961 ggcctcgccc tgcgtcagca gcaggtaggc gacctcgacg ccgcggtcgg tccattcggc
     7021 cacagcagcc gaggcgccgt actccaggtc gtcgggatgg gccaccacac acaacacgcg
     7081 gcggaagtcc tcgtcgggca gcggttcgat gctcggtgct tcagtcatgg cgccaaccta
     7141 ccgacctcgt cgagcggcac tggcacccgg gacggcttcc ggcacccggg acggctgtcg
     7201 gcttccggct tccggcaccc ggcttccagg ctcagccgag ccgatccttg aagacctgcc
     7261 aacgctggcc gtctgccgga gccatgaacg tccaatcggt tcgggtgacg ccaccgttgg
     7321 accagtacct ggtcgtgtga ccgtggacgg agtacgtgct ggtgccgaac tcgcagctgt
     7381 atttccagcc gctgggcacg gcaacgggct caaccgaccc acccttggcg gaccatcgat
     7441 acgtgagttc gaccccggcg cttgcttcga cgttcgtgaa tacccaggtc gtgaactcgt
     7501 cggtcaatcc ggggtacgtg gccggcagcg acgaggacgg cggctcggag caggtcagcg
     7561 gcgccgtctg gccgatggtg ttgcggaagg agcgctggta cgcggtgtgc caggacgcgg
     7621 ccggctcgga gtcgatgacg gcgcattcca cacgatcgga ccgggtcacg cactgtgctg
     7681 aacccttgat cgcagcgtgc gctgagccca ccgggagcca gacggtaagg gcggatgccg
     7741 ccgccaatgc tgcgactgtt cgcgcggcac ggcgaccgat tgccatctga ttcctccagt
     7801 accccagccg gatagcggaa cgttatcggt ccgggcaacc gtgagcgtcc atcgagcgtg
     7861 aaccttgacg caactcttat ccaaggtccg tctcatccac ctgcgccgag tggtgctaga
     7921 gcgccccgag ggcccttgcc acgctgattc ccgccgccgc cgcccaggcc tgcggacgac
     7981 acgacgccgg gtagggcacc ggcgggaaca ctttgtcggc gctctggccg ccgaacagct
     8041 ccggcaggcg gtggtcgaag ccctcggccg cccgcaacag cccgcgcgcc aggctcgcgg
     8101 cctcgtccgg ataaccggca cgcagcatgc cctcgatgat catcgcggtg tcgtgggtcc
     8161 acaccgagcc gacgtggtac cgggtcggcc agtaggcggc gttggtggtg gacatcgtcc
     8221 gaacaccgta gccagagaac atgtcctggc ccatcaggcg gcgcacgacg gtctgttcct
     8281 cggccgggtt cagcaagccg gtgcccagca ggtggcccat gttcgaggcc acgccggtga
     8341 ccgggcgctt gtgccggtcg agggccatcg ccgggtaggc cccgcgctcg tccgcgatcc
     8401 agaaccgctg ccggaaccgg tcggcgggtt gctgcgccca gtcgcgccgc ccggtggccg
     8461 cgtcgggatc ggttccgaac cgctccagca ggtcggcgcc ggccagggcg gctgcgtagg
     8521 cgtagccctg cacctcggcg agtgcgatcg ggccctcggc gatggtgccg tcggcgaacc
     8581 ggaccgaatc gcccgaatcc ttccagccct ggttggacag gccgtggccc gaggtgtcga
     8641 agtactcggc gaagccgtcc ccgtcggcgt cggacgagtc gcgcagccag cccagcgcgg
     8701 cctccagggg ctcgaacagc ccctcggccc gcgggtcgat gccggtgtcg gccagttcgc
     8761 cgagcagcac gatccacaac gcggtcgcat cgatcgtgcc gtagtactcg gggggcagga
     8821 cgatgccctg ctgcaggtcg agtgcctcgg cgcgcacctc gtgcaggatc ttgcccacct
     8881 gttcggcccg gtcgatgtcg acgccgcgtc cctgacgcgc ggccagcgtg cgcagggtgc
     8941 cgagcgccgg cccgctgtcg acgggcagca acagacgtgc cgcgatcaac gagtcccgac
     9001 cgaacagggt caggaaccac ggtgccccgg cggcgaaaaa ggtgtgcgac gggtggtccc
     9061 gctcagccag tcgcagcgcg ttcaggtcgg cgatcgagcg ctgcaccagg cgctgcagcg
     9121 aggccgaggc gtccaccaga gtcggcacct gcagcggctc ggattgcgca gccacgaacg
     9181 gcagccggtc gtcgcgggcc gccaactgcc agcccacggt caccgagccg taggccggca
     9241 cggtgcactg ccagcacagg tgcacggaat caccggtgct
//
LOCUS       DAOSUG010000042         9209 bp    DNA     linear   ENV 10-JUN-2024
DEFINITION  MAG TPA_asm: Micropruina sp. isolate AMR_MDS_2195
            NODE_4007_length_9209_cov_4.584881, whole genome shotgun sequence.
ACCESSION   DAOSUG010000042 DAOSUG010000000
VERSION     DAOSUG010000042.1
DBLINK      BioProject: PRJNA925477
            BioSample: SAMN32864262
            Sequence Read Archive: SRR4236673
KEYWORDS    WGS; ENV; Metagenome Assembled Genome; MAG; Third Party Data; TPA;
            TPA:assembly.
SOURCE      Micropruina sp. (wastewater metagenome)
  ORGANISM  Micropruina sp.
            Bacteria; Bacillati; Actinomycetota; Actinomycetes;
            Propionibacteriales; Nocardioidaceae; Micropruina.
REFERENCE   1  (bases 1 to 9209)
  AUTHORS   Abdulkadir,N., Saraiva,J.P., Zhang,J., Stolte,S., Gillor,O.,
            Harms,H. and Rocha,U.
  TITLE     Genome-centric analyses of 165 metagenomes show that mobile genetic
            elements are crucial for the transmission of antimicrobial
            resistance genes to pathogens in activated sludge and wastewater
  JOURNAL   Microbiol Spectr 12 (3), e0291823 (2024)
   PUBMED   38289113
REFERENCE   2  (bases 1 to 9209)
  AUTHORS   Abdulkadir,N.
  TITLE     Direct Submission
  JOURNAL   Submitted (24-MAR-2023) Environmental Microbiology, Helmholtz
            Center for Environmental Research -UFZ, Permoserstr 15, Leipzig
            04318, Germany
COMMENT     The annotation was added by the NCBI Prokaryotic Genome Annotation
            Pipeline (PGAP). Information about PGAP can be found here:
            https://www.ncbi.nlm.nih.gov/genome/annotation_prok/
            
            ##Genome-Assembly-Data-START##
            Assembly Method        :: SPAdes v. 3.11.1
            Genome Representation  :: Full
            Expected Final Version :: Yes
            Genome Coverage        :: Not Applicable
            Sequencing Technology  :: Illumina MiSeq
            ##Genome-Assembly-Data-END##
            
            ##Genome-Annotation-Data-START##
            Annotation Provider               :: NCBI
            Annotation Date                   :: 05/04/2023 04:57:10
            Annotation Pipeline               :: NCBI Prokaryotic Genome
                                                 Annotation Pipeline (PGAP)
            Annotation Method                 :: Best-placed reference protein
                                                 set; GeneMarkS-2+
            Annotation Software revision      :: 6.5
            Features Annotated                :: Gene; CDS; rRNA; tRNA; ncRNA
            Genes (total)                     :: 2,776
            CDSs (total)                      :: 2,750
            Genes (coding)                    :: 2,714
            CDSs (with protein)               :: 2,714
            Genes (RNA)                       :: 26
            tRNAs                             :: 24
            ncRNAs                            :: 2
            Pseudo Genes (total)              :: 36
            CDSs (without protein)            :: 36
            Pseudo Genes (ambiguous residues) :: 0 of 36
            Pseudo Genes (frameshifted)       :: 2 of 36
            Pseudo Genes (incomplete)         :: 32 of 36
            Pseudo Genes (internal stop)      :: 2 of 36
            ##Genome-Annotation-Data-END##
FEATURES             Location/Qualifiers
     source          1..9209
                     /organism="Micropruina sp."
                     /mol_type="genomic DNA"
                     /submitter_seqid="NODE_4007_length_9209_cov_4.584881"
                     /isolate="AMR_MDS_2195"
                     /isolation_source="Activated sludge of municipal
                     wastewater treatment plants"
                     /db_xref="taxon:2737536"
                     /environmental_sample
                     /geo_loc_name="Canada"
                     /lat_lon="49.25 N 123.1 W"
                     /collection_date="2015-06-16"
                     /metagenome_source="wastewater metagenome"
                     /note="metagenomic"
     gene            complement(<1..3554)
                     /locus_tag="PLF56_02460"
     CDS             complement(<1..3554)
                     /locus_tag="PLF56_02460"
                     /inference="COORDINATES: protein motif:HMM:NF015497.2"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_component: GO:0005737 - cytoplasm [Evidence IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="SpvB/TcaC N-terminal domain-containing protein"
                     /protein_id="HOQ52457.1"
                     /translation="MNTPQTIGLRATGVTLSVVLVLGLGGGTPALADNWKAKPQQTAS
                     VEVTKVAKATVRVPTTASYVPADVTWPAATQATVALTDTAAGRMARAGTSPMKVGVPD
                     GAKPQKAATATVQVLDRASSDTLVGLGVVATVTASAGAPVQVSMDYSGFASAGGAGFG
                     SRLQLVTLPGCALTTPQRVECQVQTPLETVNDPAARVLTATLPGTGAGPQVSPSATPT
                     GSPSAAPSITSTPSTSASPPKALPTSSSQPSVSSSFTPGGAAKSVTVLAAAAATTSEQ
                     GDYTATSLKPSAAWAAGSMSGEFTWSYPIAAPDVPGEVSPDLSIGYSSGGTDGGVSNT
                     NNQASWVGEGFELGSAFIERKYAGCYDDRSGGTNSSSTASDLCWYTDSKKTNDQKWDN
                     AFLSMAGHAGELVRIGNTAEWRLEVDDGTRVAKIGTAASNNEYWRVTTPDGTQYFFGK
                     GKADGAATATNATWSVPVSGNHSAEPGYNSSFASSFVLRPWRWNLDYVVSPTGVTMTY
                     FYVKETNKYKKNLATSTSYDRGGYLSKIQYGERKGAETVDDSPAKVTFTVEERCDTTI
                     SSGCKTTKPTSSTAKAWPDVPMDAVCDADYCPSQKTAPTFFSRKRLKQVDTYTRNAAG
                     TAWEAVDSWVLAGSFPKPSDGGAVPSLWLSSITRTGKAASTSIALPAVNLSPIMLDSR
                     ISGAGVALEKPRLAVVTSETGAQTIVEYSHPECTSTTLPSSGDIPTNTKRCMPVYYSA
                     GTAAPSLQWFNKFVVLSVTARDPVGQADSSTVPAGLDIGFDQVTSYTYTGGGAWRYND
                     SPLIKAKYRTWGEWRGFGKITTIAGKGTTKEVDETLYFRGMNGDRATSTGGTKTVTVS
                     DSTGGTWPDDDWLAGTIRETRSLTAVGGSEDTGSLMDPAVRATMSGTAPAADGRLTSH
                     QIDVGKTITRQKTPTGTRTGSDTVLAWDQFGQPTLAEDQGDLAVTGDETCTRTSYATP
                     TSASTGPIALVAEESVSPGTCAAALDYATVLSATRHHYGTTNHTDTVTIPGLETKTVQ
                     LRDGNPRPWVNTGTASYDTWGRPLTATDPLSNTTSTSYSHTTGGLLSATTTTSPDPDG
                     AGALTPHTTTVSYDTRLGAPIKTVQPGGQTSEAALDALGRVTSVWQPGQPKTSPASAT
                     YSYTISKTTTSSVTSKTLLPTGTTYTTTIALLDSILRP"
     gene            complement(3572..5278)
                     /locus_tag="PLF56_02465"
     CDS             complement(3572..5278)
                     /locus_tag="PLF56_02465"
                     /inference="COORDINATES: ab initio
                     prediction:GeneMarkS-2+"
                     /note="Derived by automated computational analysis using
                     gene prediction method: GeneMarkS-2+."
                     /codon_start=1
                     /transl_table=11
                     /product="hypothetical protein"
                     /protein_id="HOQ52458.1"
                     /translation="MQFGWPTRLPAAVVDGATATYPEVYPGVDLVVQAGLESVETFLV
                     VKSRAAALNPAVRAWSMPMTVPPGMSAKALPNGGKVVIDAKGLERFQVPQALMWDSSG
                     KPDGSSSTVDLIADVAESRVAPVDASMAAGRLSLGVKGSFLDDPATVFPVVIDPAASL
                     SQTHVLRVTDDWSKWDDAVGDHGKIGYNGWTSPYYRSRMFYQFAWPVSGLKSSQIRSG
                     VFEYVQDHSTQHSPCVTTSTAFPGVRAKLANAIDSSDTWSDRTGDAWHPQASVFSRMA
                     VGHEDYCNDTYREKWNLTGALQIERTSYDYRTTVTVGLYSDDEADRDGWKHYLNEGTS
                     PKLVLTYEKEPLVPAVADLAVSAAPVPQTSPRRTLASTPTLSSKVSLESGYNCQSSTA
                     CLQAEFEVTGGPSTISINGARVTQSNQISTTAALPAMTAGTYNVRVRARNVDTNLVSG
                     WSASIPLVVDLAPAAPSWSWLPPVVQASDNLPAASTLQISATAASADADVTQLCAVIV
                     QDSSTSEVCANATGTPKAATITAGPFVAGKSYAVTVAAKDPYQSGVAKADTPSVRAVP
                     VQ"
     gene            6125..7249
                     /locus_tag="PLF56_02470"
     CDS             6125..7249
                     /locus_tag="PLF56_02470"
                     /inference="COORDINATES: protein motif:HMM:NF019350.2"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_component: GO:0016020 - membrane [Evidence IEA];
                     GO_function: GO:0000155 - phosphorelay sensor kinase
                     activity [Evidence IEA];
                     GO_function: GO:0046983 - protein dimerization activity
                     [Evidence IEA];
                     GO_process: GO:0000160 - phosphorelay signal transduction
                     system [Evidence IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="histidine kinase"
                     /protein_id="HOQ52459.1"
                     /translation="MRFLNSNWVEVVLATLLSSLALILDLRGGHMVTLILDLAACAAA
                     ATTARWPRAGSAVLAALLAGHLLVPREWSTMGKYAALIPILGTGIRGNRQLRVVISVV
                     YLVILTAVQYHDYPERGALFLFAGLTWGVLFAACWVIGDLFSSLRRNHAASERAALAE
                     QRLTLARDLHDTIARSITRLSLTARLARDSGNPAELDTVIDDIHQIGNELRWVMTVLN
                     DPTQTRLAESSTGSLQSTISGIQASLTKHGFPTTVTLEGDDLAIIPEPIQHALNDVFA
                     EAAANIDRHAKRGRPCAILGQVDAYQAEFLIINEHDPTDATTGTHMGLDGMRQRLHPY
                     SGAIETRQENPRWTLHITVPLPPTRSASSSSMTTSTSEPA"
     gene            7162..7812
                     /locus_tag="PLF56_02475"
     CDS             7162..7812
                     /locus_tag="PLF56_02475"
                     /inference="COORDINATES: protein motif:HMM:NF012301.2"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_function: GO:0003677 - DNA binding [Evidence IEA];
                     GO_process: GO:0000160 - phosphorelay signal transduction
                     system [Evidence IEA];
                     GO_process: GO:0006355 - regulation of DNA-templated
                     transcription [Evidence IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="response regulator transcription factor"
                     /protein_id="HOQ52460.1"
                     /translation="MDTAHHSAPAADKVRVIIIDDDLYVRTSLSSILNKSHQVTCIAT
                     YATGQRAIAALPTDKAQVALVDINMPDTDGPATTRALHQAAPDLHILALTSLADEQSA
                     AAMIDAGATGFLAKDLPTPSMINAICAAATGLNVLSGPASRLIATNPYRDRPQLDDTE
                     QQLLSLVMDGRSNNEIAQTIHLAESTVKYHLTGLMAKFHVHNRTKLAVRAAELGYH"
     gene            7974..>9209
                     /gene="nhaA"
                     /locus_tag="PLF56_02480"
     CDS             7974..>9209
                     /gene="nhaA"
                     /locus_tag="PLF56_02480"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_015068972.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_function: GO:0015385 - sodium:proton antiporter
                     activity [Evidence IEA];
                     GO_process: GO:0006814 - sodium ion transport [Evidence
                     IEA];
                     GO_process: GO:1902600 - proton transmembrane transport
                     [Evidence IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="Na+/H+ antiporter NhaA"
                     /protein_id="HOQ52461.1"
                     /translation="MNQSADGRWPARLRRFVTGETSGGILLLGAAAIGFGWANSPASD
                     AFTALAEHRIGPALLHLDLSLATWAADGLLAIFFFVVGVELKHELVAGSLRHPREAGV
                     PVVAAVGGMALPAAIFTVLVLLLGDRTALQGWAVPTATDIAFALAVLAVFGRGLPRAL
                     RTFLLTLAVVDDLLAIVVIAVFYTDRINLWTLLAAALVIVGFGIAVRARSLRWWVLLP
                     LAVVAWALMHDSGVHATIAGVALGLTVPALVRGAESEPRTHRLDDAVRPWSAGVALPV
                     FAFFAAGVTLVGRGADGFDPSVFVAVMTGLVLGKLIGVLGATALVTRFTPLRLPDAIG
                     LRDLLPVGLLCGIGFTVSLLIAELSFDDQAHIVAAKTGVLLASVLAAVLGALTLRWDA
                     RQARSRDMNADGAPDRDVRE"
BASE COUNT         1552 a         3174 c         2886 g         1597 t
ORIGIN      
        1 ctgggccgca ggatgctgtc cagtagcgcg atcgttgtgg tgtaggtggt gccggtcggc
       61 agcagcgttt tggaggtcac cgaactggta gtggtcttgc tgatcgtgta gctgtaggta
      121 gcggaggccg gactggtctt gggttggccg ggttgccaca ccgacgtgac ccggcccagg
      181 gcgtcgaggg cggcttcgct ggtttgaccg cccggctgca cagttttgat cggggcaccg
      241 aggcgggtgt cgtagctgac ggtggtggta tgcggggtca gtgcaccggc accgtctgga
      301 tccgggctgg tggtggtagt cgcgctgagc agtccgccgg tggtgtggct gtagctggta
      361 ctcgtggtgt tactgagcgg atcggtcgcg gtgagcggcc gtccccaggt gtcgtagctt
      421 gcggtgccgg tgttcaccca tggccgcggg ttcccgtcgc gcaactgcac cgttttggtt
      481 tccagtccgg gaatggtgac ggtgtcggtg tggttggtgg tgccgtaatg gtgccgggtg
      541 gccgacaaca cagtggcgta gtcgagcgcg gcggcgcacg ttcccgggct caccgactcc
      601 tccgccacca atgcgatcgg gcccgtgctg gcgctggtcg gggtggcgta gctggtgcgg
      661 gtgcaggttt cgtcgccggt gacggccagg tcgccctggt cttcggcgag ggtgggttgc
      721 ccgaactgat cccatgccag aacggtgtcg ctgccggtgc gggtacctgt cggtgtcttc
      781 tgccgggtga tcgtcttgcc cacatcgatc tggtgggagg tgagccggcc gtcggcggcc
      841 ggagcggtgc cggacatggt ggcccgaacg gctgggtcca tcaaagaacc ggtgtcctcg
      901 ctgccgccga ctgcggtcag gctgcgggtt tcgcggatcg tgccggccag ccagtcatca
      961 tcaggccagg tgccgccggt ggagtcgctg acggtgacgg ttttggtgcc gccggtgctg
     1021 gtggcccggt cgccgttcat gccgcggaag tacagcgttt cgtcgacctc tttggtggtg
     1081 cccttgccgg cgatggtggt gatcttgccg aacccgcgcc actcgcccca ggtgcggtac
     1141 ttggccttga tcaacggaga gtcgttgtaa cgccaggcgc cgccaccggt gtaggtgtag
     1201 ctggtcacct gatcgaaccc gatatccaac cctgccggaa cagtggagga gtcggcctgg
     1261 ccgaccggat cccgtgcggt caccgacaac acgacgaatt tgttgaacca ctgcaggctg
     1321 ggggcggcgg tgccggccga gtagtagacc ggcatgcacc gtttcgtgtt ggtggggatg
     1381 tcacctgagc tgggcagggt ggtggaggtg cattcggggt gggaatactc cacgatcgtc
     1441 tgcgcaccgg tttcggaggt caccaccgcc agtcgtggtt tctccagcgc gaccccggca
     1501 ccgctgatgc gggagtcgag catgataggc gacaggttga ctgcgggtaa cgcgatcgag
     1561 gtactggccg ctttgccggt ccgggtgatc gacgacaacc acaacgacgg caccgctccc
     1621 ccgtcggacg gctttgggaa cgagccggcc agtacccagc tgtccacggc ctcccaggcg
     1681 gtgccggcag cgttgcgggt gtatgtgtcg acctgcttga gccgtttgcg ggagaagaac
     1741 gtcggagcag ttttctgtga tgggcagtag tcggcgtcgc acaccgcgtc catcggcaca
     1801 tccggccacg ccttcgccgt cgacgaggtc ggcttcgtcg tcttgcagcc tgatgagatg
     1861 gtggtgtcgc aacgctcctc cacggtgaac gtcaccttcg ccggcgaatc gtccaccgtt
     1921 tcggcgcctt tgcgttcgcc gtactggatc ttcgacagat acccgccccg gtcgtagctg
     1981 gtggaggtgg ccaggttctt cttgtacttg ttcgtttcct tgacgtagaa gtaggtcatg
     2041 gtcacacccg tgggcgacac cacatagtcc aggttccagc gccacgggcg cagcacgaac
     2101 gaactggcga agctcgagtt gtaacccggc tcggctgagt gattgcccga caccggcacc
     2161 gaccacgtcg cgttggtcgc ggtcgccgcc ccatcggcct tgcctttgcc gaagaagtat
     2221 tgggtgccgt cgggggtggt gacccgccaa tactcattgt tggaggcggc ggtgccgatc
     2281 ttcgcgaccc gggtgccgtc gtccacctcc agacgccact cggccgtgtt accgatccgc
     2341 accaactcgc cggcatgacc ggccatcgac aagaacgcgt tgtcccactt ctggtcgttg
     2401 gtcttcttcg aatcggtgta ccagcacaga tcggaggcgg tcgaggagga gttggtgccg
     2461 ccggaacgat cgtcgtaaca gccggcgtat ttgcgttcga tgaacgccga acccagctcg
     2521 aagccttcac ccacccagga ggcctggttg ttcgtgttcg acacgccacc atcggtgccg
     2581 cccgacgaat agccgatcga caggtccggt gacacctcgc ccggcacgtc gggggcagcg
     2641 atcggatacg accaggtgaa ctcacccgac atgctgccgg ccgcccaggc cgccgacggc
     2701 ttcaacgacg tcgccgtgta gtcaccctgc tcgctggtcg tcgccgcagc cgccgccagg
     2761 accgtcacag acttcgccgc accaccgggg gtgaagctcg aggacaccga cggctgggag
     2821 gacgaagttg gcaacgcctt tggcggcgac gctgatgtgc tcggtgtgga tgtgatcgac
     2881 ggcgccgcgg aaggcgaccc ggtgggggtg gcggacggtg acacctgcgg gccggcaccg
     2941 gtgccgggca gagtggcggt gagcacgcgg gcagcgggat cgttgacggt ctccagcggt
     3001 gtctgcacct ggcattcgac acgctgtgga gtggtcagcg cgcagccggg cagcgtgacc
     3061 agttgcagac ggctgccgaa cccggcaccg ccggcggagg cgaaccccga atagtccatg
     3121 ctgacctgca ccggagcccc cgctgaagcg gtgacggtgg cgacaacacc cagcccgacc
     3181 aatgtgtcgc tgctggcacg gtccagcacc tgcacggtag cggtggcggc cttctgcggc
     3241 ttggcaccgt ccggcacacc gaccttcatc gggctggtcc cggcgcgggc catccgtccc
     3301 gcggcagtgt cagtcaatgc aacggtggcc tgcgtcgccg ccggccaggt cacatccgct
     3361 ggcacgtagc ttgctgtggt cggcaccctc acagtggctt tggcgacctt cgtcacctcc
     3421 acgctggcgg tctgctgcgg cttcgccttc cagttgtccg ccaacgccgg tgtgccgcca
     3481 cccaatccca acaccaacac gacgctcaac gtcacgccag tggcccgcaa cccgatggtt
     3541 tgtggagtgt tcatcctgac cccgtctgtt gtcactgcac cggcacggcg cgaacactcg
     3601 gcgtgtccgc cttcgccaca cccgactgat acggatcctt cgccgccacg gtgaccgcat
     3661 aggacttgcc ggccacgaac ggtccggccg tgatggtggc cgccttgggt gtcccggtgg
     3721 cgttggcgca cacctcactg gtcgagctgt cctggacgat caccgcgcac agctgagtga
     3781 catccgcatc ggctgacgcc gcagttgcag agatctggag cgtgctggcg gcaggcagat
     3841 tgtcgctggc ctgcaccacc ggcggcagcc acgaccaaga cggtgcggcc ggtgccaggt
     3901 ccaccactaa tgggatcgac gccgaccacc ccgacaccag gttggtgtcc acattcctgg
     3961 cccgcacccg cacgttgtag gtccctgcgg tcatcgcagg cagggcggcg gtggtggaga
     4021 tctgattgct ttgggtgacc cgcgcaccgt tgatcgagat cgtggatggg ccaccggtca
     4081 cctcaaactc ggcctgcagg caggctgtgc tcgactggca gttgtagccc gattccagcg
     4141 acaccttcga cgacaacgtg ggtgtggagg cgagcgtgcg gcgcggcgag gtttgcggca
     4201 ctggagccgc cgacacagcc aaatcggcca ccgccggcac caacggctcc ttctcatacg
     4261 tcaacaccaa cttgggagag gtgccttcgt tgaggtaatg cttccaaccg tcccgatccg
     4321 cctcatcatc ggagtacaaa ccgaccgtga ccgtggtgcg gtagtcgtag ctggtgcgct
     4381 caatctgcaa cgcgccggtc aggttccact tctcccgata cgtgtcattg cagtagtcct
     4441 catgcccaac cgccatccgt gagaacaccg acgcctgcgg atgccacgcg tcccctgttc
     4501 ggtccgacca agtatcggag ctgtcgatcg cattcgccaa cttcgcccgc acccccggga
     4561 aagccgttga ggtggtcacg cacgggctgt gctgggtcga gtggtcctgc acatactcga
     4621 acacacccga gcggatctgg gacgacttca gccccgacac cggccaggcg aactggtaga
     4681 acatccgcga ccggtaataa ggagacgtcc agccgttgta gccgatcttg ccgtggtcac
     4741 cgaccgcgtc gtcccacttc gaccaatcat cagtgacccg caacacatgg gtttgcgata
     4801 acgatgcggc tgggtcgatc accaccggga atactgtcgc cggatcatcc agaaacgaac
     4861 ctttcacccc aagcgacaat ctgccggccg ccatcgacgc atcgacgggt gcgacccgcg
     4921 actctgcaac atctgcgatc agatcgaccg tgctagacga tccgtcgggt ttgcctgacg
     4981 aatcccacat caacgcctgc ggcacttgga aacgctcgag ccctttggcg tcgatgacca
     5041 ctttgccgcc attgggcagc gccttcgccg acatccctgg gggcaccgtc atcggcatcg
     5101 accatgcccg caccgcagga ttcaaagccg ccgcccggga cttgaccacc agaaacgtct
     5161 cgaccgattc caagcccgcc tgcaccacca agtccacccc cggatacacc tccggatagg
     5221 ttgctgtggc cccgtccaca accgcagccg gcagcctcgt cggccagccg aactgcaacg
     5281 atgcagaccc gtccgacaac acagcaagcg gagccgacga aggcccgccc gggctgatcg
     5341 cgatctgcgc tacggcggcc tcaggccgca gcaacccatc cgaaccgcgc accaacttcg
     5401 ttgacggggc ccgccaacca ccctgcggac ccttcaccct ggccacattc gcggtcagct
     5461 ccgcgatgaa ctgacccgag gacggatcgg ctgtgacgag cgtccgctca tcggtcaacg
     5521 ccgacaccac caccggcgaa cccgtcgcct tcgcctgcct caacgcttca gactcgctga
     5581 gcaggccacc ttccgccgtc ggaacgggcg tcggtgtcgg tgccgggtcg gccagcgcca
     5641 cagaaacgtg gccacaaccg agcgcgaacg ctaggcaaga caccgtgacg gcaacacgga
     5701 ctcgcgcatt ggtgaacatg accagatccc ccgttgttcg gttgaagacg tttgcgttgc
     5761 gcgttcagac agactggtca gcagccgggc cagaacggaa agtagtcaca cacgccggtg
     5821 ccgacggcct ggctacctga gtggctactt gaagctggcg cgacgagcag tgcgccggcg
     5881 gtggtaagcg caacgacgat caacaccgac cgcgaaaccc gttgcccctt aaaaccctgt
     5941 ccccgaaagc tgtgcatggc gcaatgctgt gcgcagccta tggtactggg aagcgattcg
     6001 cagaagaatt gaccgggtgg atagcgaagt cggcaccacc gactgtccag gtggacaacc
     6061 ctcctcccta aagaccggcc aatcgtctag cgtgccaacc atcgcggtga agaaggagtg
     6121 aacagtgcgg ttcctcaaca gcaactgggt cgaggtcgtc ctcgccacct tgctgtccag
     6181 cctcgcgctc atcctggacc tacgcggcgg ccacatggtg acgctcattc tcgaccttgc
     6241 agcctgtgca gcagccgcca ctactgcacg ctggccgcga gccggctcgg cggtcctcgc
     6301 ggcactactc gccggccacc tgctggtccc gagggagtgg tcaaccatgg gcaagtacgc
     6361 ggccctgatc ccgatcctcg gaaccggcat ccgcggtaac aggcaactcc gcgttgtgat
     6421 cagcgtcgtc tacctcgtca ttctcaccgc tgtgcagtac cacgactacc ccgaacgcgg
     6481 cgcactgttc ctgttcgcag ggctcacctg gggtgttctc ttcgccgcct gctgggtcat
     6541 cggcgatctg ttcagctcgc tgcgccgcaa ccacgccgca tccgaacggg ctgcgctggc
     6601 cgagcaacgc ctgaccctgg cccgcgacct gcacgacacc atcgcccgca gcatcaccag
     6661 gctctccctc accgcccgcc tcgcacgcga cagcggcaac ccggccgaac tggacacagt
     6721 catcgacgac atccaccaaa tcggcaacga actgcgctgg gtcatgaccg tactcaacga
     6781 ccccacccaa acccggctcg cggaatcatc caccggcagc ctgcaaagca ccatctccgg
     6841 aatccaggcc agcctgacca aacacggatt ccccaccacc gtcacactcg aaggcgacga
     6901 cctcgccatc atccccgagc cgatccaaca cgcgctcaac gacgtgttcg ccgaggccgc
     6961 cgccaacatt gaccggcacg ccaaacgcgg gcggccgtgc gccatcctcg gccaggtcga
     7021 tgcctaccag gccgagttcc tgatcatcaa cgaacacgat cccaccgacg ccaccaccgg
     7081 cacacacatg ggactggacg ggatgcggca gcgcctacac ccctattctg gtgcgatcga
     7141 gacgcgacag gagaacccac gatggacact gcacatcaca gtgcccctgc cgccgacaag
     7201 gtccgcgtca tcatcatcga tgacgacctc tacgtccgaa ccagcctgag cagcattctc
     7261 aacaagtccc accaagtcac ctgcatcgcc acctacgcca ccggtcagcg cgccatcgca
     7321 gcccttccca ccgacaaggc ccaggtcgct ctcgtcgaca tcaacatgcc cgacaccgac
     7381 ggaccagcta ccactcgagc cctacaccag gcggcccccg acctgcacat cctcgccctg
     7441 acatcgttgg ccgacgagca gtccgccgcc gccatgatcg acgcaggcgc caccgggttc
     7501 ctcgccaaag acctacccac cccgtccatg atcaacgcca tctgcgccgc cgccaccgga
     7561 ctcaacgtgc tatccggacc cgccagccga ctcatcgcca ccaaccccta ccgcgaccga
     7621 ccacaactcg acgacaccga acaacaactc ctcagcctcg tcatggacgg acgcagcaac
     7681 aacgaaatcg cccaaaccat ccacctcgcc gaatccaccg ttaaatacca cctcaccgga
     7741 ctcatggcca agttccacgt ccacaaccgc accaaactcg ccgtccgagc cgccgaactc
     7801 ggctaccact gagaccgggc cctcagccga tgccccgaca tggctgcgca aagctggaaa
     7861 tctctcacca ctgagtgttg ccgatggcca tgccgacgcg aggttggggc acactagcca
     7921 acgcgccgct gtgccatctt cctgcatccg acaacaacgg cgcgggagtg ccgatgaacc
     7981 agagcgccga cgggcgatgg ccagcccggc tacgccgctt cgtgaccggc gagacctccg
     8041 gcggcattct gctgctcggc gcagcggcca tcggattcgg atgggcgaac tcgccggcca
     8101 gcgacgcgtt caccgcactg gccgagcacc ggatcgggcc ggcgctgctg cacctcgatc
     8161 tatcgctggc cacctgggcc gcggacggac tgctcgccat cttcttcttc gtggtgggcg
     8221 tcgagctcaa acacgaactg gtagccggca gcctgcgcca cccgcgcgag gccggggtgc
     8281 cggtggtggc agcggtgggc ggcatggctt tgccggcagc gattttcacg gtgctcgtgc
     8341 tgctgctggg cgaccgcacc gctctgcagg gctgggccgt tcccaccgcg accgacatcg
     8401 cgttcgcgct ggccgtactc gcggtgttcg gccgtgggct tccgcgcgcc ctgcgcacct
     8461 ttctgctcac cctcgccgtg gtggacgacc tgctggccat cgtcgtgatc gccgtcttct
     8521 acaccgaccg gatcaacctg tggacgctgc tcgccgcagc cctcgtgatc gtcggcttcg
     8581 gtatcgccgt ccgggcccgc tcgctgcgct ggtgggtact gctaccgctc gccgtcgtgg
     8641 cgtgggcgct gatgcacgac agtggtgtgc acgcgaccat cgccggggtg gcgctcggcc
     8701 tgaccgtgcc ggcgctcgtc cggggcgccg aatcggaacc tcgcacccac cgcctggacg
     8761 acgccgtcag gccgtggtcg gcaggggtgg cgctgccggt gttcgcgttc ttcgccgccg
     8821 gcgtcacgct ggtcggacgc ggcgcggacg gctttgatcc gtcggtgttc gtcgccgtga
     8881 tgaccggatt ggtgctgggc aagctgatcg gcgtgctggg tgccaccgcc ctggtcaccc
     8941 gattcacgcc gcttcgcctg ccggacgcca tcggcctgcg cgacctgctg ccggtcggcc
     9001 tgttgtgcgg catcgggttc acggtgtcgc tgctgatcgc cgagttgtcg ttcgacgatc
     9061 aggcgcacat cgtggccgcc aagaccggcg ttctgctggc cagcgtgctc gcagccgtgc
     9121 tgggcgcgct cacccttcgc tgggacgccc ggcaggcccg ttcgcgcgac atgaacgcgg
     9181 acggggcgcc cgaccgcgac gtccgtgaa
//
LOCUS       DAOSUG010000043         9198 bp    DNA     linear   ENV 10-JUN-2024
DEFINITION  MAG TPA_asm: Micropruina sp. isolate AMR_MDS_2195
            NODE_4018_length_9198_cov_3.923548, whole genome shotgun sequence.
ACCESSION   DAOSUG010000043 DAOSUG010000000
VERSION     DAOSUG010000043.1
DBLINK      BioProject: PRJNA925477
            BioSample: SAMN32864262
            Sequence Read Archive: SRR4236673
KEYWORDS    WGS; ENV; Metagenome Assembled Genome; MAG; Third Party Data; TPA;
            TPA:assembly.
SOURCE      Micropruina sp. (wastewater metagenome)
  ORGANISM  Micropruina sp.
            Bacteria; Bacillati; Actinomycetota; Actinomycetes;
            Propionibacteriales; Nocardioidaceae; Micropruina.
REFERENCE   1  (bases 1 to 9198)
  AUTHORS   Abdulkadir,N., Saraiva,J.P., Zhang,J., Stolte,S., Gillor,O.,
            Harms,H. and Rocha,U.
  TITLE     Genome-centric analyses of 165 metagenomes show that mobile genetic
            elements are crucial for the transmission of antimicrobial
            resistance genes to pathogens in activated sludge and wastewater
  JOURNAL   Microbiol Spectr 12 (3), e0291823 (2024)
   PUBMED   38289113
REFERENCE   2  (bases 1 to 9198)
  AUTHORS   Abdulkadir,N.
  TITLE     Direct Submission
  JOURNAL   Submitted (24-MAR-2023) Environmental Microbiology, Helmholtz
            Center for Environmental Research -UFZ, Permoserstr 15, Leipzig
            04318, Germany
COMMENT     The annotation was added by the NCBI Prokaryotic Genome Annotation
            Pipeline (PGAP). Information about PGAP can be found here:
            https://www.ncbi.nlm.nih.gov/genome/annotation_prok/
            
            ##Genome-Assembly-Data-START##
            Assembly Method        :: SPAdes v. 3.11.1
            Genome Representation  :: Full
            Expected Final Version :: Yes
            Genome Coverage        :: Not Applicable
            Sequencing Technology  :: Illumina MiSeq
            ##Genome-Assembly-Data-END##
            
            ##Genome-Annotation-Data-START##
            Annotation Provider               :: NCBI
            Annotation Date                   :: 05/04/2023 04:57:10
            Annotation Pipeline               :: NCBI Prokaryotic Genome
                                                 Annotation Pipeline (PGAP)
            Annotation Method                 :: Best-placed reference protein
                                                 set; GeneMarkS-2+
            Annotation Software revision      :: 6.5
            Features Annotated                :: Gene; CDS; rRNA; tRNA; ncRNA
            Genes (total)                     :: 2,776
            CDSs (total)                      :: 2,750
            Genes (coding)                    :: 2,714
            CDSs (with protein)               :: 2,714
            Genes (RNA)                       :: 26
            tRNAs                             :: 24
            ncRNAs                            :: 2
            Pseudo Genes (total)              :: 36
            CDSs (without protein)            :: 36
            Pseudo Genes (ambiguous residues) :: 0 of 36
            Pseudo Genes (frameshifted)       :: 2 of 36
            Pseudo Genes (incomplete)         :: 32 of 36
            Pseudo Genes (internal stop)      :: 2 of 36
            ##Genome-Annotation-Data-END##
FEATURES             Location/Qualifiers
     source          1..9198
                     /organism="Micropruina sp."
                     /mol_type="genomic DNA"
                     /submitter_seqid="NODE_4018_length_9198_cov_3.923548"
                     /isolate="AMR_MDS_2195"
                     /isolation_source="Activated sludge of municipal
                     wastewater treatment plants"
                     /db_xref="taxon:2737536"
                     /environmental_sample
                     /geo_loc_name="Canada"
                     /lat_lon="49.25 N 123.1 W"
                     /collection_date="2015-06-16"
                     /metagenome_source="wastewater metagenome"
                     /note="metagenomic"
     gene            complement(<1..1421)
                     /locus_tag="PLF56_02485"
     CDS             complement(<1..1421)
                     /locus_tag="PLF56_02485"
                     /EC_number="2.7.1.30"
                     /inference="COORDINATES: protein motif:HMM:NF000756.0"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_function: GO:0004370 - glycerol kinase activity
                     [Evidence IEA];
                     GO_function: GO:0005524 - ATP binding [Evidence IEA];
                     GO_process: GO:0006071 - glycerol metabolic process
                     [Evidence IEA];
                     GO_process: GO:0016310 - phosphorylation [Evidence IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="glycerol kinase"
                     /protein_id="HOQ52462.1"
                     /translation="MTKYILGIDSGTTSVRSFVIDEAATVIGFGQAEITQIYPRPGWI
                     EHDAKQILGTQLEVMRAALRDAGVQPDEISAAGITNQRETAVVWDKSTGEPIYNAVVW
                     SSRQTVPVIEQWVEQGIGDLIEERTGLIPDAYYSASKIRWILDHIAGAQERAEKGELL
                     AGTIDTWLIWNFTNGKSFVTDVSNGSRTMMMNLKTLDWDEELLGHYNIPRHMLAQIVP
                     SDANFGDLEPTFGAPIPIGSDLGDQQAGLFGQAAFEQGQCKMTYGTAGVLNINCGQSP
                     QRIAGLTPSVGWTVQGTTAYEIEGVLFAQGKTMQWLRDDLQLIHAAPDSEWYAGQVKS
                     TDGVYLVPAFTGLSAPTWDPYARAAIIGISNATTRLHIIRAAVESMVYQTRDMVEAAT
                     ADGSIKIPELRIDGGAVKNNLLCQMQADILGIPVIRPKNHEATIFGAMLLAGLSTGIY
                     SSLDELQGMWAVERVFEPQLSRD"
     gene            1586..2407
                     /locus_tag="PLF56_02490"
     CDS             1586..2407
                     /locus_tag="PLF56_02490"
                     /EC_number="3.-.-.-"
                     /inference="COORDINATES: protein motif:HMM:NF019887.2"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_function: GO:0016787 - hydrolase activity [Evidence
                     IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="HAD family hydrolase"
                     /protein_id="HOQ52463.1"
                     /translation="MLDRLDLSAVRAVALDVDGTLAGADSKVSPRTIAAMKRVQELGI
                     PVILLTGRTRRNTMAIARQASLRSFAVACNGAVIIDPVTDENVAVNPMAAADKATFRR
                     LADDLGLDLTWWTENHMYVPGRGDMYDLILELNHEEALVGDPDDIADAVVMKMMAMSS
                     VEHMDEVQDEILRRMPTAMRSLDNLVEVVDPDSTKWHGLEWVLERYGVDAANVLGAGD
                     GGNDVVWLSRIGFPVAMGNARDEVREVTVAEGLTNADEGAADLLERVAAAHGVAA"
     gene            2404..3672
                     /locus_tag="PLF56_02495"
     CDS             2404..3672
                     /locus_tag="PLF56_02495"
                     /inference="COORDINATES: protein motif:HMM:NF012246.2"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_function: GO:0000287 - magnesium ion binding [Evidence
                     IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="RuBisCO large subunit C-terminal-like
                     domain-containing protein"
                     /protein_id="HOQ52464.1"
                     /translation="MSAQVMLAELARKGDYLLATYALDLPADVDPWARVSRFAVGQTT
                     GTWVEVPGLTDELRSVHEGRVVQVLPVPASDTTTHAGGDTISYLATLAFPTMNFGASL
                     PQLLNTLIGNDASTSVEAKLVDLQLPGSLESAFGGPRFGIAGVRELTGVSDRPLLLNM
                     LKPCTGLAPADAAAIFQATALGGIDLIKDDELMGNTPYSTVLERVKLFTAAADEAFDT
                     TGVRTVYLPNISDRPDRMLETAQRAVEAGARALMVAYTTVGLGAVEALADVVGVPILG
                     HFAGAAPLYEGPRTGMSAPLATGLLPRLAGADLALVVSPYGGYPITRLQYLRTAHQLS
                     LPRPHLKPALPVIGGGVHPGTVTRFVGELGSDIVLGVGGAIQGHPDGAAAGVRAMRQA
                     VDAALAGVSAEDAAKEHEELATALELWGTL"
     gene            3778..5580
                     /locus_tag="PLF56_02500"
     CDS             3778..5580
                     /locus_tag="PLF56_02500"
                     /inference="COORDINATES: protein motif:HMM:NF021489.2"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_component: GO:0005886 - plasma membrane [Evidence IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="DUF2207 domain-containing protein"
                     /protein_id="HOQ52465.1"
                     /translation="MRRFIVVLVGCATLLLGLAAAPRALADSSDDSITRYAVTADAAK
                     DGTTTVTLDFDFNFGSDSGLGPYITLPLRQEITNDPDHWRMLDVTPGAVTSPSGANAE
                     VQTETQDGNLLIRVGNEYRTYTGTQTYRITYTIRGLIAPNQATSGLDEFNWNLIGPSW
                     EVPIRNITAKVTGPADVTNTACFVGGGLTATCSSPSTSDGATATFAQRSVSPGEPVQI
                     VAGFPAGTFVGAEARLEKRLTIGNMFPITPVTGSIAAVLTAVGLGALLRRTRRGARDE
                     VYLGLTPGVVPAPGQAATIGRGSVDAPVAVQFTPPRGVRPGEIGVLTDSTADNVDITA
                     TIIDLAVRKHLRIEQQGKRDWTFVRLSDTDQLNDYESYLLTQLFQNGSTVSTDDLKNG
                     DYSGVLEGTRERLYTRVTSELQWFRGNPRMVRAAAIVLGLLLVAIGAGLGFALGLVGF
                     GLIGVAVAICGLAMMALNSRFGSRTANGSAVLAQAKGFELYLTTAEADQIKFEEGIDI
                     FSRYLPYAIVFGVADRWTKIFEQLAAQGRYVADNSWYLGYGYGTFFSSGFTSSLDQLA
                     STMSTSMQASTAATSGGSGFSGGGGFGGGGGGGW"
     gene            complement(5599..6075)
                     /locus_tag="PLF56_02505"
     CDS             complement(5599..6075)
                     /locus_tag="PLF56_02505"
                     /inference="COORDINATES: ab initio
                     prediction:GeneMarkS-2+"
                     /note="Derived by automated computational analysis using
                     gene prediction method: GeneMarkS-2+."
                     /codon_start=1
                     /transl_table=11
                     /product="hypothetical protein"
                     /protein_id="HOQ52466.1"
                     /translation="MISPEPLTAPTAVTLEAAPTAVVRHGGVTMATLRPLFDSGFTAL
                     ASSGAAIDGPAFAWYRGDTGAEFDLEIGFPLAEPLGQPVPGVETVEPSRLPSGSAWVL
                     SHLGAYGSLHESWGRLDEHARRRGLTPTGMLEVYVTQPSPELDPTTLRTDLFLLTE"
     gene            complement(6125..6490)
                     /locus_tag="PLF56_02510"
     CDS             complement(6125..6490)
                     /locus_tag="PLF56_02510"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_005885516.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_function: GO:0003677 - DNA binding [Evidence IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="helix-turn-helix domain-containing protein"
                     /protein_id="HOQ52467.1"
                     /translation="MAQPAWDPYASDCPSRQLLDRIGDRWTVLVIGTLSQGPLRFTEL
                     CRGIDGISQKMLTQTLRTLERDGLVTRTVHAEVPPRVEYVLTEAGRSLQQPLRALEQW
                     TIDHMDAVVRARRRYDAAR"
     gene            6602..7246
                     /locus_tag="PLF56_02515"
     CDS             6602..7246
                     /locus_tag="PLF56_02515"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_017935403.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="NAD(P)H-binding protein"
                     /protein_id="HOQ52468.1"
                     /translation="MAHIVIIGGTGYAGSHIAAEAVARGHAVTSWSRSLPEQSLEGVS
                     YRTGSVTDDATLSEAVDGADVVIETLSPRGSMLGQVEPVAAGLAKKLTGTKTRLGVIG
                     GAGSLQVSPGGPRVMDGDQFPDAFKAEAAELGRVLDALRASDQGLDWFFVSPAAGFGA
                     YAPGERTGHYRVGGDVLLVDEAGRSDISGADLAIAIVDEIERPAHHRRRFTVAY"
     gene            complement(7596..8780)
                     /locus_tag="PLF56_02520"
     CDS             complement(7596..8780)
                     /locus_tag="PLF56_02520"
                     /EC_number="2.3.1.-"
                     /inference="COORDINATES: protein motif:HMM:NF025054.2"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_function: GO:0008080 - N-acetyltransferase activity
                     [Evidence IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="GNAT family N-acetyltransferase"
                     /protein_id="HOQ52469.1"
                     /translation="MARSPIAGTPRPRRGRPAAPTVRVEHPHDPAPQVAATRPHSVIT
                     PAERAFFVEEFRGVTIVVGLPVLTDEAVRGLARTVSGYEAGDTRFVLVVRSDDDAARV
                     AEPIHAGRTVPAPLQWNADALAELWIATTDARVLVVVAEAAALAAARIAAGLGASKLV
                     LTEPGGGWGDPPRSFAELRRHRRPLERALAERDAPGLLDAVELALRGGAFSVNLCRPE
                     DVELELFTFDGAGTLITLNGYLGVSDLRVDDFAAVEDLVARGVDTGILKPRSRQQIAR
                     MMVGGLGARVIRTGHLAGLVGLEVDAYGTVGVGEVSGLVTVSEFSGAGAGGLLIDALL
                     NRARDQGLSRVFAVTVSDEAATFFLHRGFTEVSRDEVPAGKWAGYAPERLARARCFNW
                     VL"
     gene            complement(8813..>9198)
                     /locus_tag="PLF56_02525"
     CDS             complement(8813..>9198)
                     /locus_tag="PLF56_02525"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_015583597.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_function: GO:0046912 - acyltransferase activity, acyl
                     groups converted into alkyl on transfer [Evidence IEA]"
                     /codon_start=3
                     /transl_table=11
                     /product="citrate/2-methylcitrate synthase"
                     /protein_id="HOQ52470.1"
                     /translation="MGFGHRIYKNFDPRAQIVKQHADAILRERPGNDELLDLALALEE
                     AALSDDYFAERKLYPNVDFYTGLIYQAMGFPANMFTVLFAIGRLPGWIAHWREQYLDP
                     VKKIGRPRQVYVGEVERDYVPLDAR"
BASE COUNT         1406 a         3155 c         3235 g         1402 t
ORIGIN      
        1 tcgtcgcggc tgagttgcgg ctcaaacacg cgctcgaccg cccacatgcc ctgcagctcg
       61 tccagtgagc tgtagatgcc ggtggacagg ccggccagca acatggcgcc gaagatcgtc
      121 gcctcgtgat tcttcggccg aatcaccgga ataccgagaa tgtcggcctg catctggcag
      181 agcaggttgt tcttcaccgc gccgccgtcg atgcgcaact cgggaatctt gatcgagccg
      241 tcggcggtgg ccgcctcgac catgtcgcgg gtctggtaga ccatcgactc caccgcggcc
      301 ctgatgatgt gcagccgggt ggtggcgttg ctgatgccga tgatcgcggc ccgggcgtag
      361 ggatcccagg taggggccga caggccggtg aaggcgggca ccaggtagac cccgtcggtg
      421 ctcttgacct gcccggcgta ccactccgag tcgggcgcgg catgaatcaa ctgcaggtcg
      481 tcgcgcagcc attgcatggt cttgccctgg gcgaagagca caccctcgat ctcgtaggcg
      541 gtcgtgccct gcaccgtcca gccgacggac ggggtgaggc cggcgatccg ctgcggggac
      601 tgtccgcagt tgatgttcag cacacccgcc gtgccgtagg tcatcttgca ctggccctgc
      661 tcgaacgcgg cctggccgaa cagtccggcc tgctggtcgc cgaggtcgga cccgatcgga
      721 atgggtgccc cgaacgtggg ttcgagatcg ccgaagttgg catcgctggg cacgatctga
      781 gccaacatgt gccgcggaat gttgtagtgg ccgagcagtt cctcgtccca atccagcgtc
      841 ttgaggttca tcatcatggt gcgcgaaccg ttggagacgt cggtgacgaa cgacttgccg
      901 ttggtgaagt tccagatcag ccaggtgtcg attgtgccgg ccaacagctc gcccttctcg
      961 gcccgctcct gcgccccggc gatgtggtcg aggatccagc ggatcttgga cgcgctgtag
     1021 tacgcgtccg gaatcagccc ggtgcgctct tcgatcaggt cgccgatgcc ctgctcgacc
     1081 cactgctcga tcacgggcac ggtctgccgc gacgaccaga ccacagcgtt gtagatcggc
     1141 tcgccggtgg acttgtccca caccaccgcg gtctcgcgct ggttggtgat gccggcggcg
     1201 ctgatctcgt ccggctggac tccggcgtcg cgcagggcgg cccgcatcac ctcgagctgc
     1261 gtgccgagga tctgcttggc gtcatgctcg atccagcccg gccgtgggta gatctgggtg
     1321 atctcggcct ggccgaagcc gatcaccgtg gcagcctcgt cgatcacgaa cgaacgcacg
     1381 ctggtggttc ccgagtcgat gccaaggatg tatttcgtca tgtagcccct cacgtcggtg
     1441 tggttgggtg taactggacg cggtcgcggc ccgtccgtct cgggcagcag ccaaggcccc
     1501 accctaaaga gggtgtgtag cacaggtcaa cccttgacca tacttgtcta tacgtgtagt
     1561 ctgccggact cctgaaagga cccgcatgct ggaccgactc gacctttccg ccgtgcgcgc
     1621 cgttgcgctc gacgtggacg ggaccctggc cggtgccgat tcgaaggtga gcccccgcac
     1681 catcgccgcg atgaagcggg tgcaggagct ggggataccg gtgatcctgc tcaccggacg
     1741 tacccggcgc aacaccatgg cgatcgcccg acaggcgtct cttcgcagct tcgccgtggc
     1801 ctgcaacggc gcggtgatca tcgacccggt caccgatgag aacgtggcgg tgaacccgat
     1861 ggcggccgcc gacaaggcga cgtttcggcg gctggccgac gatctgggac tagacctgac
     1921 ctggtggacc gagaaccaca tgtacgtccc cggccgcggt gacatgtacg acctgattct
     1981 cgagctgaat cacgaggaag cactggtggg tgaccccgac gacatcgcgg acgcggtggt
     2041 gatgaagatg atggcgatga gcagcgtcga gcatatggac gaggtgcagg acgagatcct
     2101 gcgtcgcatg ccgaccgcga tgcggtcact ggacaacctg gtcgaggtgg tcgaccccga
     2161 ctcgacaaag tggcacggtc tggagtgggt gctggagcgc tacggcgtcg acgccgcgaa
     2221 cgtactgggc gccggcgacg gcggcaacga cgtggtgtgg ctgagccgga tcggctttcc
     2281 cgtcgcgatg ggcaatgcac gcgacgaggt gcgcgaggtc accgtggccg aagggctgac
     2341 caacgccgac gagggtgcgg ccgacctgct cgagcgggtc gcggcggcgc acggggtggc
     2401 ggcatgagcg cgcaggtgat gctggccgag ctggcccgca agggcgatta cctgctggcc
     2461 acgtacgcgc tcgacctgcc ggccgacgtc gacccatggg cgcgggtctc acggttcgcg
     2521 gtgggccaga ccaccggcac gtgggtcgag gtgccggggc tgaccgacga gttgcggtcg
     2581 gtacacgagg gtcgggtggt gcaggtgctg ccggtcccgg cgtccgacac caccacgcac
     2641 gcgggcggtg acacgatcag ctacctggcc acgctggcgt tcccgacgat gaacttcggc
     2701 gcctcgctgc cgcagctgct gaacacgctg atcggcaacg acgcgtccac gtcggtggag
     2761 gccaagctgg tcgacctgca gctgcccggc tcgttggaga gcgcgttcgg cgggccgcgg
     2821 ttcggcatcg ccggggtgcg cgagctgacc ggggtgagcg atcgtccgct gctgctgaac
     2881 atgctcaagc cgtgcaccgg actggcgccg gccgatgcgg cggcgatctt ccaggcgacc
     2941 gcgctgggcg gcatcgatct gatcaaggac gacgagttga tgggcaacac gccctactcg
     3001 actgtgctcg agcgggtgaa gctgttcacc gcggcggccg acgaggcgtt cgacaccacc
     3061 ggggtgcgca cggtgtacct gccgaacatc tcggatcgtc ccgaccggat gctcgagacg
     3121 gcccagcgcg cggtcgaggc gggcgcacgg gcgctgatgg tggcctacac caccgtcggg
     3181 ctgggtgcgg tcgaggcgct ggcggacgtg gtcggcgtgc cgatcctcgg gcacttcgcg
     3241 ggggccgcgc cgctgtacga ggggccgcgg acggggatga gcgcacccct ggcgaccggg
     3301 ctgctgcctc ggctggccgg ggccgacctg gccctggtgg tctcgcccta cggcggttac
     3361 ccgatcaccc ggctgcagta tttgcggacc gcgcatcagt tgtcattgcc ccgtccgcac
     3421 ctgaagccgg cactcccggt gatcggcggg ggcgtccatc ccggcacggt gacccggttc
     3481 gtcggcgaac tgggcagcga catcgtgctc ggggtcggtg gcgccatcca gggccatccg
     3541 gacggcgcgg cggcgggcgt ccgcgcgatg cgccaggcag tggacgcagc actggccggc
     3601 gtcagcgccg aggacgcagc gaaggagcac gaagaactgg cgacggcact ggagctctgg
     3661 ggcacgctgt agtggggcgg gtgcgtccga cgcaccggtc ggtccggtcg aacgctcgcg
     3721 ttgcagccgc tgactccgta gcggacggga gcgagcaccg gcttgtaccg ttgtggcatg
     3781 cgacggttca tcgtggtgct ggtcggttgt gcaacgctgc tgctcgggtt ggccgccgcg
     3841 ccgagggccc tggccgattc gtccgacgac agcatcaccc gctacgcggt gaccgcggac
     3901 gcggcgaagg acggcaccac caccgtcacg ctcgacttcg acttcaactt cggcagcgac
     3961 tcggggctcg ggccctacat caccctgccg ttgcggcaag agatcacgaa cgaccccgac
     4021 cactggcgca tgctcgacgt caccccgggc gcggtgacca gcccgtccgg cgccaacgcc
     4081 gaggtgcaga ccgaaaccca ggacggcaac ctgctgatcc gggtcggcaa cgagtaccgc
     4141 acctacaccg gcacccagac ctatcgcatc acgtacacga tccgtggcct gatcgcaccc
     4201 aaccaggcca cgtcggggct ggacgagttc aactggaacc tgatcggtcc cagctgggag
     4261 gtgccgatcc gcaacatcac cgccaaggtc accgggccgg cggacgtcac caacaccgcc
     4321 tgcttcgtgg gcggcggact gaccgcgacc tgttcgtcgc cgtccacctc ggacggcgcg
     4381 accgccacct tcgcccagcg ttcggtgtct ccgggcgaac cggtgcagat cgtcgccggt
     4441 tttccggccg gcaccttcgt cggagccgag gcacggctcg agaagcggtt gacgatcggc
     4501 aacatgttcc cgatcacgcc cgtgaccggc agcatcgcgg ccgtgctcac cgcagtcggg
     4561 ctgggggcgc tgttgcgccg cacccgccgc ggcgcgcgcg atgaggtgta cctcggcctg
     4621 acgcccgggg tcgtgcccgc accgggccag gcggccacca tcggacgcgg ctccgtggac
     4681 gcaccggtcg ccgtgcagtt caccccgccc aggggcgtcc gtccgggcga gatcggcgtg
     4741 ctgaccgact cgaccgccga caacgtcgac atcaccgcca cgatcatcga tctcgcggta
     4801 cgcaagcacc tgcggatcga gcagcagggc aagcgcgact ggaccttcgt gcggctgagc
     4861 gacaccgacc agctcaacga ttacgagtcg tacctgctca cccagctgtt ccagaacggc
     4921 tcgacggtca gcaccgacga cctcaagaac ggcgactact ccggggtgct cgagggcacc
     4981 cgcgagcggc tctacacccg ggtcacgtcc gagcttcagt ggttcagggg caacccgcgc
     5041 atggtgcgcg ccgcggccat cgtgctgggg ctgctcctgg tggcgatcgg tgccggcctg
     5101 ggctttgcgc tcggcctggt cgggttcggc ctgatcgggg tggcggtggc gatctgcggt
     5161 ctggcgatga tggcgctgaa cagccggttc ggttcacgca ccgccaacgg ttcggccgtg
     5221 ctggcgcagg ccaagggctt cgagttgtac ctgaccacgg cagaggccga ccagatcaag
     5281 ttcgaagagg gcatcgacat cttcagccgg tacctgccct acgccatcgt gttcggcgtg
     5341 gccgatcgct ggacgaagat cttcgagcag ctcgccgccc aggggcgcta cgtggctgac
     5401 aactcctggt acctcggcta cggctacggc accttcttca gcagcggctt cacctcgtcg
     5461 ctcgatcagc tcgcgagcac gatgtcgacc tcgatgcagg cctcgacggc agcgaccagc
     5521 ggcggctcgg gattcagcgg cggcggcggt ttcggcggtg gtggcggcgg cggctggtga
     5581 cccggggcgc tccgggcctc actcggtgag caaaaacagg tcggtgcgca gcgtggtcgg
     5641 atccagctcc ggcgagggct gggtcacgta gacctcgagc atgccggtcg gcgtcagccc
     5701 gcgccgcctg gcgtgttcgt cgagtcgtcc ccaactctcg tgcagcgacc cgtaggcgcc
     5761 caggtgactg agcacccagg ccgagccgga gggcagtcgc gacggttcaa cggtctcgac
     5821 gcccggtacg ggctggccca gcggttcggc cagcggaaac ccgatctcga ggtcgaactc
     5881 ggcgccggtg tcgccgcggt accacgcgaa ggccggcccg tcgatcgcgg caccagagct
     5941 cgctagggcg gtgaacccgc tgtcgaacaa cggacgtagc gtggccatcg tcaccccgcc
     6001 atgccgcacc accgcggtgg gcgcggcctc cagagtcacc gcggtaggag cggtcagggg
     6061 ctcgggactg atcatgcgtc gatcgtgcac gaggtgatcg cgacacacca gccccggacg
     6121 ggtgtcaccg ggccgcgtcg tacctgcgcc gcgcccgcac cacggcgtcc atgtggtcga
     6181 tcgtccactg ctcaagagcg cgcaggggct gctgcaggct gcggccggcc tcggtgagca
     6241 cgtactccac ccgcggcggc acctcggcat gcacggtgcg cgtgaccagg ccgtcccgct
     6301 ccagggtgcg cagggtctgg gtgagcatct tctgcgagat gccgtcgata ccacggcaca
     6361 gttccgtgaa ccgcagcggc ccctggctca gggtgccgat caccagcacc gtccagcggt
     6421 caccgatgcg gtcgagcaac tggcggctgg gacagtcgct ggcgtaggga tcccaggccg
     6481 gttgggccat cgggcacctt ctttccgtgc tctgttgctg atggttagta actcacctat
     6541 ggtaactaat ggcgctcact agtgaaagag cgccgaaact cgcaacgaga ggcaaccttc
     6601 catggcacac atcgtcatca tcggcggaac cggttacgcc ggcagtcaca tcgccgcaga
     6661 ggccgtcgcg cgcggccacg cggtcacctc gtggtcgcgc agcctgcccg agcagtccct
     6721 ggagggcgtc agctaccgca ccggttctgt gaccgacgac gccacgctca gcgaggccgt
     6781 ggacggtgcc gacgtggtca tcgagaccct gtcgccccgc ggcagcatgc tcggacaggt
     6841 cgaacccgtc gcggccggcc tggcgaagaa gctgaccggc accaagaccc ggctgggcgt
     6901 gatcggtggt gccggctcgc tgcaggtcag ccccggaggc ccccgggtga tggacggcga
     6961 tcagttcccc gacgcgttca aggccgaggc cgccgagttg ggccgcgtgc tggacgcgtt
     7021 gagggcctcc gaccagggcc tggactggtt cttcgtcagc ccggccgcgg gcttcggcgc
     7081 ctacgctccc ggcgagcgca cgggtcacta ccgggtgggc ggcgacgtgc tgctggtcga
     7141 cgaggccggc cgcagcgaca tctcgggcgc cgacctggcc atcgcgatcg tggacgagat
     7201 cgagcggccc gcgcaccacc gccggcgctt cacggtcgcc tactgatcag aggccggccg
     7261 cgggtgagcg gcgacgtcga gcggcgcggg catgatgcgg gtcgccaagc tggccccggc
     7321 cgactggccc gcgtcgcact cgcggcgaaa acgtcgcagc gcgtcccgca accgcgcgcc
     7381 cacacgtgcc tcacggagca ccgaccggcc cgtgaggcac gtgtgcgacg ccctgctgga
     7441 tgtgtgcgac gccctcgcct cgaccggacc gcccctcgct cagccgcgcg acacagccgc
     7501 tcaccgaccc ggtatcgccg gcggcggtgc tgcgcgccag gctcgtcacc cggcgcctgc
     7561 ggcagctgag cgactgaacc cccctggccc gagcgctaca ggacccagtt gaagcagcgg
     7621 gcccgggcca gccgctcggg agcgtacccc gcccacttgc ccgccggcac ctcgtcacgc
     7681 gacacctcgg tgaagccgcg gtgcagaaag aaggtggcgg cctcgtccga gaccgtgacg
     7741 gcgaacaccc tgctcagccc ctggtcccgg gcccggttga gcagggcgtc gatgagcagg
     7801 ccgccggcgc ccgcgcccga gaactcgctg accgtgacca gtcccgacac ctcgccgacc
     7861 ccgacggtgc cataggcgtc cacctcgagg ccgaccagcc cggccaggtg cccggtgcgg
     7921 atcacccggg cgcccaggcc gccgaccatc atgcgggcga tctgctgtcg gctgcgcggt
     7981 ttcagaatgc cggtgtcgac acccctggcg accaggtcct cgacggcggc gaagtcgtcg
     8041 acccgcaggt cgctgacgcc caggtagccg ttcagggtga tcagagtgcc ggccccgtcg
     8101 aaggtgaaca gctccagttc gacgtcctcg gggcggcaca ggttcaccga gaacgcgccg
     8161 ccgcgcaacg ccagttcgac ggcatcgagc agcccggggg cgtcgcgctc ggccagggcg
     8221 cgctccagcg gacgccggtg ccggcgcagt tcggcgaagc ttcggggcgg gtcaccccag
     8281 ccgccacccg gttcggtgag caccagcttg gacgccccga ggccggccgc gatgcgcgcc
     8341 gccgcgagag cggcagcctc ggccaccacc accagcaccc gggcatccgt ggtggcgatc
     8401 cagagctcgg ccagcgcgtc ggcattccac tgcagcggcg ccggcacggt gcgtccggca
     8461 tggatgggtt cggccacccg cgcggcgtcg tcgtcgctgc gcaccacgag cacgaagcgg
     8521 gtgtcgccgg cctcgtagcc gctgaccgtt cgggccagcc cgcgcaccgc ctcgtcggtg
     8581 agtaccggca gcccgaccac gatcgtgacg ccgcggaact cctcgacgaa gaacgcgcgc
     8641 tcggccggcg tgatcaccga gtgcggccgg gtggccgcca cttggggcgc cgggtcgtgc
     8701 ggatgctcca cccgcacggt cggcgccgcc ggccgacccc ggcgcggacg cggggtgccg
     8761 gcgatcggtg agcgtgccat ggggcacagt gtggcagcgc ggccctgggc ggctagcggg
     8821 cgtccagcgg cacgtagtcg cgctcgacct caccgacgta gacctgccgc ggacgaccga
     8881 tcttcttgac cggatcgagg tactgctcgc gccagtgggc gatccagccg ggcagccgtc
     8941 cgatggcgaa cagcacggtg aacatgttcg ccggaaagcc catcgcctgg tagatcaggc
     9001 cggtgtagaa gtcgacgttg gggtacagct tgcgctcggc gaagtagtcg tcgctgagcg
     9061 ccgcctcctc gagcgccagg gcgaggtcga gcaactcgtc gttgcccggg cgctcgcgca
     9121 ggatcgcgtc cgcgtgttgc ttcacgatct gggcgcgggg gtcgaagttc ttgtagatgc
     9181 ggtgcccgaa gcccatca
//
LOCUS       DAOSUG010000044         9183 bp    DNA     linear   ENV 10-JUN-2024
DEFINITION  MAG TPA_asm: Micropruina sp. isolate AMR_MDS_2195
            NODE_4021_length_9183_cov_4.295793, whole genome shotgun sequence.
ACCESSION   DAOSUG010000044 DAOSUG010000000
VERSION     DAOSUG010000044.1
DBLINK      BioProject: PRJNA925477
            BioSample: SAMN32864262
            Sequence Read Archive: SRR4236673
KEYWORDS    WGS; ENV; Metagenome Assembled Genome; MAG; Third Party Data; TPA;
            TPA:assembly.
SOURCE      Micropruina sp. (wastewater metagenome)
  ORGANISM  Micropruina sp.
            Bacteria; Bacillati; Actinomycetota; Actinomycetes;
            Propionibacteriales; Nocardioidaceae; Micropruina.
REFERENCE   1  (bases 1 to 9183)
  AUTHORS   Abdulkadir,N., Saraiva,J.P., Zhang,J., Stolte,S., Gillor,O.,
            Harms,H. and Rocha,U.
  TITLE     Genome-centric analyses of 165 metagenomes show that mobile genetic
            elements are crucial for the transmission of antimicrobial
            resistance genes to pathogens in activated sludge and wastewater
  JOURNAL   Microbiol Spectr 12 (3), e0291823 (2024)
   PUBMED   38289113
REFERENCE   2  (bases 1 to 9183)
  AUTHORS   Abdulkadir,N.
  TITLE     Direct Submission
  JOURNAL   Submitted (24-MAR-2023) Environmental Microbiology, Helmholtz
            Center for Environmental Research -UFZ, Permoserstr 15, Leipzig
            04318, Germany
COMMENT     The annotation was added by the NCBI Prokaryotic Genome Annotation
            Pipeline (PGAP). Information about PGAP can be found here:
            https://www.ncbi.nlm.nih.gov/genome/annotation_prok/
            
            ##Genome-Assembly-Data-START##
            Assembly Method        :: SPAdes v. 3.11.1
            Genome Representation  :: Full
            Expected Final Version :: Yes
            Genome Coverage        :: Not Applicable
            Sequencing Technology  :: Illumina MiSeq
            ##Genome-Assembly-Data-END##
            
            ##Genome-Annotation-Data-START##
            Annotation Provider               :: NCBI
            Annotation Date                   :: 05/04/2023 04:57:10
            Annotation Pipeline               :: NCBI Prokaryotic Genome
                                                 Annotation Pipeline (PGAP)
            Annotation Method                 :: Best-placed reference protein
                                                 set; GeneMarkS-2+
            Annotation Software revision      :: 6.5
            Features Annotated                :: Gene; CDS; rRNA; tRNA; ncRNA
            Genes (total)                     :: 2,776
            CDSs (total)                      :: 2,750
            Genes (coding)                    :: 2,714
            CDSs (with protein)               :: 2,714
            Genes (RNA)                       :: 26
            tRNAs                             :: 24
            ncRNAs                            :: 2
            Pseudo Genes (total)              :: 36
            CDSs (without protein)            :: 36
            Pseudo Genes (ambiguous residues) :: 0 of 36
            Pseudo Genes (frameshifted)       :: 2 of 36
            Pseudo Genes (incomplete)         :: 32 of 36
            Pseudo Genes (internal stop)      :: 2 of 36
            ##Genome-Annotation-Data-END##
FEATURES             Location/Qualifiers
     source          1..9183
                     /organism="Micropruina sp."
                     /mol_type="genomic DNA"
                     /submitter_seqid="NODE_4021_length_9183_cov_4.295793"
                     /isolate="AMR_MDS_2195"
                     /isolation_source="Activated sludge of municipal
                     wastewater treatment plants"
                     /db_xref="taxon:2737536"
                     /environmental_sample
                     /geo_loc_name="Canada"
                     /lat_lon="49.25 N 123.1 W"
                     /collection_date="2015-06-16"
                     /metagenome_source="wastewater metagenome"
                     /note="metagenomic"
     gene            <1..1290
                     /locus_tag="PLF56_02530"
     CDS             <1..1290
                     /locus_tag="PLF56_02530"
                     /EC_number="2.3.1.9"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_007077867.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_function: GO:0016746 - acyltransferase activity
                     [Evidence IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="acetyl-CoA C-acetyltransferase"
                     /protein_id="HOQ52471.1"
                     /translation="ASMAALRNAVIVGGNRVPFGRIGGAYRSATNLDLLTASIDGLAA
                     RFGLAGERIGEVGAGAVLKHSRDFNLTRESVLSSALAASTPAYDVQQACATSLAAAVQ
                     LSNRIRVGQLEAAIAGGVDSASDSPIAVNDRLRKKLLRLNAAKTPLERVKTLASVRPS
                     DLAPEVPRAAEPRTGLNMGQHQALTTLQWGITREAQDELAAASHHNLAKAWADGFFDD
                     LVTPYLGQSIDQSLRADTSVEKLATLRPVFGDGTDATMTAGNSTPLSDGAAVVLLADE
                     AWARQRNLPALARIVDAETAAVDYLLGNEGLLMAPAHATARLLARNELALQDFDLYEI
                     HEAFASTVLCTLAAWESDDYCRDTLGLDGALGSIDRAKLNVTGSSLAAGHPFAATGAR
                     ILATLAKLLAQRGRGSRGLISVCAAGGQGVVAIVEAV"
     gene            1290..2669
                     /locus_tag="PLF56_02535"
     CDS             1290..2669
                     /locus_tag="PLF56_02535"
                     /EC_number="1.1.1.100"
                     /inference="COORDINATES: protein motif:HMM:NF006110.0"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_function: GO:0016491 - oxidoreductase activity
                     [Evidence IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="3-oxoacyl-ACP reductase"
                     /protein_id="HOQ52472.1"
                     /translation="MTLDSLLSAALGSPLGKKAMAAAGFAEATMLRRGELMPSGPLVL
                     GVAGDGTAVAEALGALGLAAQPALIDAPETRSPDQQGRQRPPSYPQRIGAVLVDASRL
                     ATVPQLEQVRAVLRPAIRALEPSGRVIIVGGDPASTADVEQAAVRQALDGIMRSVGKE
                     LRAGATSNLIQLADTTTADQLGEVLHFLLSGRSAYVNGQPLVLADLATAPAPSARIVV
                     VTGAARGIGAAIARRFAAGGDRVVVIDVPAAGEALAAVANELHGTALQLDITVPDAGA
                     RIAEHVASRYGADARIGALVHCAGILRDKMLANMDEARWASVLQVNLAAQLRINEVLL
                     DESVAGGLADGSRIVAIASTSGWAGNKGQTNYAASKAGVMGLVRALAPRLAGRGIVVN
                     GVAPGFIETDMTASIPVIDREIFRRSSSLLQGGKPVDVAEAIVFLADPATPLNGQVLR
                     VCGQLIVGR"
     gene            2642..3631
                     /locus_tag="PLF56_02540"
     CDS             2642..3631
                     /locus_tag="PLF56_02540"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_017933724.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="MaoC/PaaZ C-terminal domain-containing protein"
                     /protein_id="HOQ52473.1"
                     /translation="MRAADRGSLMAQVLDLKAMPPALGLYAKALAPTRRPSVAAGLPE
                     REVQLLGHRSTVTEVAAYDRVCGFVLGDELPATWLHVLTFPLQLTLMTAPGFPLALAG
                     LVHVSNSMVQVRPALIGEALDLRVRAENLRPHRRGVLVDLVGEAAVAGETVWQGRSVY
                     LSRQARLAPAPEPGASPVPSGRQEGRGVVGEASRSLSSANGSHSVPADDAAGTASAPS
                     LQMAGLWRLPADLGRRYAAVSGDVNPIHLSALSAKAFGFPRAIAHGMCTHARVLAALQ
                     PRLPQVYEVSVQFIKPVLLPSTVRLALGESEQGFSAQVTNRAADRVFLTLSTQ"
     gene            complement(4485..5687)
                     /locus_tag="PLF56_02545"
     CDS             complement(4485..5687)
                     /locus_tag="PLF56_02545"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_012921222.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="M20 family metallopeptidase"
                     /protein_id="HOQ52474.1"
                     /translation="MTATTAIAARLHPELRTLRRAIHAEPELGNDLPRTQARVLAALE
                     GLDLEITTGAALSSVVAVLRGRAETTGRRPVVLLRGDMDGLPVIEATGQQFAATNGTM
                     HACGHDLHVAGLVGAAKILHELRDQLPGDVVFMFQPGEEGPGGAGPMIAEGVLDAAGR
                     RADAAYGLHVVSSEHPFGVWSGRPGVLMAAADQCYITVRGRGGHGSQPHHAQDPVPVA
                     CEIAIALQTMVTRQFDAFDPVVVTVGKIAAGSKDNIIPDDARLESTVRTFSAANHEAI
                     GERITRLAQGIAQAHGMSADVEYVTGYPVTVNNEGEYARVVDAVVDLFGADRYVEQPF
                     PEAGAEDFSLVAQQVPSAFIFVSAYPGEDWATAASNHSPRALFDDDVLADCAALLAEV
                     TVRRLRQG"
     gene            complement(5723..6256)
                     /locus_tag="PLF56_02550"
     CDS             complement(5723..6256)
                     /locus_tag="PLF56_02550"
                     /inference="COORDINATES: ab initio
                     prediction:GeneMarkS-2+"
                     /note="Derived by automated computational analysis using
                     gene prediction method: GeneMarkS-2+."
                     /codon_start=1
                     /transl_table=11
                     /product="hypothetical protein"
                     /protein_id="HOQ52475.1"
                     /translation="MNAQRELSQSVLQVIFSVFLGLVVTAFVGIGVNTFYPEPEYVTG
                     SQPDYSQWRLITSIVLLSCATLVMLLSLAIAEAGAVLANGALLGGLFTMIYAVGLGMS
                     TANQWPRFAVMTAALAITVGVGWWRFGRGRTAAATASGSGAGGGVGAGAALPAEVEAR
                     LSAVEARLAALGRALRD"
     gene            complement(6253..7161)
                     /locus_tag="PLF56_02555"
     CDS             complement(6253..7161)
                     /locus_tag="PLF56_02555"
                     /inference="COORDINATES: ab initio
                     prediction:GeneMarkS-2+"
                     /note="Derived by automated computational analysis using
                     gene prediction method: GeneMarkS-2+."
                     /codon_start=1
                     /transl_table=11
                     /product="hypothetical protein"
                     /protein_id="HOQ52476.1"
                     /translation="MPTVALITSQYALTSPVDADREITLIGDELARRGVTVRAIDWLD
                     QTADYAACDLVVIKSPWDYAGRHDEFLDWLARVEAVAEVVNPPEVIRWNMDKRYLGEL
                     TALGVPACPTTYCAHVDEVRAALAEASGRVVLKPNVSAASADTGLFEAGDPAGVRLAE
                     QILAAGKVVMIQPAIESVGRVGERSLIYFGDEFVHAIGKGPLLALGGGLRGEQYQESI
                     VKAHAPDDERALARQALAVVERIFTDRGLAHVCPLLYARVDIARDAQDRPLLMELELF
                     EPSYFLDLAPGAERLFVDCVMARLTP"
     gene            7443..7631
                     /locus_tag="PLF56_02560"
     CDS             7443..7631
                     /locus_tag="PLF56_02560"
                     /inference="COORDINATES: ab initio
                     prediction:GeneMarkS-2+"
                     /note="Derived by automated computational analysis using
                     gene prediction method: GeneMarkS-2+."
                     /codon_start=1
                     /transl_table=11
                     /product="hypothetical protein"
                     /protein_id="HOQ52477.1"
                     /translation="MSTANPSDDLGTVLRWQAAGGEIEVLSWGPPVVVSLCTCDGGQQ
                     MQRLTSDADDLLAHLRSR"
     gene            7752..8492
                     /locus_tag="PLF56_02565"
     CDS             7752..8492
                     /locus_tag="PLF56_02565"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_007077699.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_function: GO:0005524 - ATP binding [Evidence IEA];
                     GO_function: GO:0016887 - ATP hydrolysis activity
                     [Evidence IEA];
                     GO_function: GO:0042626 - ATPase-coupled transmembrane
                     transporter activity [Evidence IEA];
                     GO_function: GO:0140359 - ABC-type transporter activity
                     [Evidence IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="amino acid ABC transporter ATP-binding protein"
                     /protein_id="HOQ52478.1"
                     /translation="MSEPLVVIENVNKHFGDLHVLKNINLSIDKGEVVILIGPSGSGK
                     STLCRTINRLETYESGSITIDGKTLPDEGKELANLRADVGMVFQSFNLFAHKTILENI
                     TLGPIKVRKTPKAEAEKKAMEPLTRVGVEAQAEKYPAQLSGGQQQRVAIARSLAMNPK
                     VMLFDEPTSALDPEMVNEVLDVMVQLAKEGMTMIVVTHEMGFARKAADKVVFLADGQI
                     VETNTPTEFFANPQSGRAKDFLSKILTH"
     gene            8554..>9183
                     /locus_tag="PLF56_02570"
     CDS             8554..>9183
                     /locus_tag="PLF56_02570"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_015069238.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="transporter substrate-binding domain-containing
                     protein"
                     /protein_id="HOQ52479.1"
                     /translation="MSLKLKLGAALAAAALALSMSACGSGAGSTNLQALKIGIKFDQP
                     GLGLKDGDKYSGFDVDVATYIAGKLGTTADKITWIQAPSAQRETLIESGQVDLVVATY
                     SITDARKEKVSFAGPYFIAGQSLLIRADDSSITGVDSLSGKKLCSVTGSTSAQKIKDK
                     VPGVNLQEFGGYSDCVTALNAKTIDALTTDDTILAGFAAQPANAGKLKLV"
BASE COUNT         1472 a         3226 c         3125 g         1360 t
ORIGIN      
        1 gcttcgatgg ctgcgctgcg caatgccgtg atcgtgggtg gtaaccgtgt tccgttcgga
       61 cggatcggcg gggcctaccg ctccgccacc aacctcgacc tgttgaccgc gtcgatcgac
      121 ggcctggcag ccaggttcgg gctcgccggg gaacgcatcg gcgaggtcgg cgcgggcgcc
      181 gtgctcaagc acagccgcga cttcaacctg acccgcgaat cggtgctgtc ctcggcgctg
      241 gcggccagta cacccgccta cgacgtgcag caggcctgcg ccaccagcct ggccgccgcc
      301 gtccagctga gcaaccggat cagagtcggc caactcgagg cggcgatcgc cggcggggtc
      361 gactcagcct ccgattcgcc gatcgcggtc aacgaccggc tgcgcaagaa gctgctgcgg
      421 ctgaacgccg ccaagacccc gctcgagcgg gtgaaaaccc ttgccagcgt gcgtccgtcc
      481 gacttggcgc ccgaggtgcc gcgggccgct gagccgcgca ccggcctcaa catgggccag
      541 caccaggcgc tgaccacgct gcagtggggc atcacccgcg aggcgcagga cgagctcgct
      601 gccgccagcc accacaacct cgcgaaggcc tgggccgacg gcttcttcga cgacctggtc
      661 accccctatc tgggccagag catcgaccag tcgctgcgcg ccgacacctc ggtcgagaaa
      721 ctggcgacgc tgcgtccggt gttcggcgac ggcaccgacg ccaccatgac cgcgggcaac
      781 tccaccccgc tctcggacgg cgcggccgtc gtgctgctcg ccgacgaggc ctgggcgcga
      841 cagcgcaacc tgccggcgct ggcccgcatc gtcgatgccg aaaccgccgc ggtcgactat
      901 ctgctcggca acgaagggct gctgatggca ccggcccacg ccaccgcccg gctgctcgcc
      961 cgcaacgagt tggccctgca agacttcgac ctgtacgaga ttcacgaggc gttcgcctcg
     1021 accgtgctgt gcacgctggc cgcctgggag tccgacgact actgccgcga caccctgggc
     1081 ctggatggtg ccctcggctc gatcgatcgg gccaagctca acgtgaccgg ctcgtccttg
     1141 gcggccgggc acccgttcgc cgccaccggc gcgcgcattc tcgccacgct ggcgaagctg
     1201 ctcgcgcagc gcggtcgcgg ctcgcgcggg ctgatctcgg tctgtgcggc cggcgggcag
     1261 ggcgtggtgg cgattgtgga ggcggtctga tgacgctcga ttcactactc tcggcggccc
     1321 tcggctcgcc cctgggcaag aaggccatgg cggccgcagg cttcgccgag gccacgatgt
     1381 tgcggcgtgg cgagttgatg ccgtccggac ccctggtgct gggggtggcc ggcgatggca
     1441 ccgcggtcgc tgaggcgttg ggcgcgctcg gtctggccgc gcagccggcc ctgatcgacg
     1501 cccccgagac gcgctccccc gaccagcagg gccgccagcg cccaccgtcc tatccgcagc
     1561 ggatcggcgc cgtgctggtg gacgcctcgc ggctggccac cgtgccgcaa ctggagcagg
     1621 tgcgggcggt gctgcgtccg gcgatccggg cgctggagcc gtccggacgg gtgatcattg
     1681 tgggcggcga cccggcctcc acggcggatg tggagcaggc ggcggtgcgt caggcgctgg
     1741 acggcatcat gcgcagcgtc ggcaaggaac tgcgcgccgg cgcgaccagc aacctgattc
     1801 agctggccga cacgaccacc gccgaccagt tgggcgaggt gctgcacttc ctgctgtccg
     1861 gacgctccgc ctacgtcaac ggccagccgc tggtgttggc cgacctggcc acggcaccgg
     1921 ccccgtccgc gcgcatcgtg gtggtcaccg gggccgcccg cggcatcggg gcggccatcg
     1981 cccggcggtt cgccgccggc ggcgaccggg tggtggtgat cgacgtgccg gcggcgggcg
     2041 aggccctggc ggcggtcgcc aacgaactgc acggcacggc tctgcaactc gacatcacgg
     2101 tgccggacgc cggcgcgcgg atcgccgagc acgtggcgtc ccgctacggg gcggacgcgc
     2161 ggatcggcgc actggtgcac tgcgccggca ttctgcgcga caagatgctg gcgaacatgg
     2221 acgaggctcg ctgggcttcg gtgctgcagg tcaacctggc cgcgcagctg cgcatcaatg
     2281 aggtgctgct cgacgaatcg gtcgccggcg ggttggcgga cggttcgcgc atcgtcgcga
     2341 tcgcctcgac cagcggctgg gccggcaaca aggggcagac gaactacgcg gcgtccaagg
     2401 ccggggtgat ggggctggtg cgtgcgctgg cgccgcggct tgccgggcgc ggcatcgtgg
     2461 tcaacggggt ggcgccgggg ttcatcgaga ccgacatgac ggcgtccata ccggtgatcg
     2521 atcgcgagat cttccggcgc agctccagcc tgttgcaggg cggcaagccg gtcgacgtgg
     2581 ccgaggcgat cgtgttcctg gccgaccccg cgacaccgct gaacggccag gtgcttcggg
     2641 tgtgcgggca gctgatcgtg ggtcgctgat ggcccaggtg ctcgacctga aggcgatgcc
     2701 gccggccctc ggcctgtacg cgaaggcact ggcgcccacc cgtcgcccga gcgtggcggc
     2761 ggggctgccc gagcgtgagg tgcagctgct cgggcaccgc agcacggtga ccgaggtggc
     2821 ggcctacgac cgggtgtgtg gcttcgtgct cggcgacgag ttgccggcga cctggctgca
     2881 cgtgctgacc ttcccgctgc agttgaccct gatgaccgcg cccgggtttc cgctggcgct
     2941 ggccggcctg gtgcatgtga gcaactcgat ggtgcaggtg cgtccggcgc tgatcggtga
     3001 ggcgctcgac ctgcgggtgc gggccgagaa cctgagaccc caccggcgcg gggtgctggt
     3061 cgacctcgtc ggcgaggctg cggttgccgg cgagacggtg tggcaggggc ggtcggtgta
     3121 cctgagtcgg caggcgcgtc tggcgccggc acccgagccg ggggcgagcc cggtgccgtc
     3181 cgggcgacag gagggcaggg gggttgttgg cgaagcatct cgctcacttt cgtcggccaa
     3241 cggcagtcat tccgtgccgg cagatgatgc cgcggggact gcatctgcgc cgagcctgca
     3301 gatggccggg ctctggcgcc ttccggcgga cctcggccgc cggtacgcag ccgtctcggg
     3361 cgatgtcaac ccgatccacc tcagtgcgct gagcgcgaag gccttcggtt ttccccgagc
     3421 gattgctcac ggcatgtgca cccacgcccg ggtgctggcg gcgttgcagc cacggttgcc
     3481 acaggtctac gaggtgtcgg ttcagttcat caagccggtg ctgctgccgt ccacggtgcg
     3541 gctggcactg ggcgagagcg aacagggatt ctcggcccag gtgaccaacc gcgcggctga
     3601 ccgggtgttt ctcacgctgt caacccagta ggcatcaccg gtcgagcccg tcgggccgag
     3661 tcgtctcgcc ctgcccgatt cgctgatgca cccagccagc ccctgaaatc cctgcgccag
     3721 tgcaaggggt ccgggcgtgc ctaggcgggc aggtggtcac tggcgcaggg atttcagggg
     3781 gtcggttcct ccacgtcacg aagatcgtgg gaggaagaag ggcttcgaca agctcgccca
     3841 gcgatggatc agcgtttccc tgaccccctt cgatcagctc gatgagtgtg gccggttctc
     3901 aggatcaaga tccgtcaacg atgtcgagat cgctacccga tggcgggaaa ggccctccag
     3961 tttcgggttg caacacgaaa ccggcgaggc gacccgccat cgagcggcga tctcgagatc
     4021 gcccgagggc cggggccagg caggctcgac cagcgacgac gatcggcgcc ggacctcgcg
     4081 caaccgtgaa gcggagtggg ggtgcacccc gtcagggcgt ggtcgggcgt aggcacactt
     4141 ctcggcggag cctgcggagc ctagaagttt gccggggcgg gacggggtgc acccccactc
     4201 cgcggcaacc aaacgcaacc cagggggcgc ggcaggcggg atctcgacaa actcgaccaa
     4261 gggagtcccc ggcatcaatt ggcagagcgc ttcggggaga tcccggcgtc cgccgggatg
     4321 acgggggaga gaattcgccc ggacggctcc aggggcggtc cggagatccc ggcgttcgcc
     4381 ggggatggcg gggagaggat tcgcccggac gactccaggg gcggtccgga gatcccggcg
     4441 cccgccggga tgacagaagc cgggtctcga ccagcgggac cccctcagcc ctggcgcagg
     4501 cgccgcaccg tcacctcggc caggagcgct gcgcagtcgg cgagcacgtc gtcgtcgaac
     4561 agggcccgtg gtgagtggtt ggacgccgcc gtggcccagt cctcgccggg gtaggccgag
     4621 acgaagatga acgcgctcgg cacctgctgc gcgaccaggc tgaaatcctc cgccccggcc
     4681 tcgggaaagg gctgctccac gtaccgatcg gcgccgaaca ggtcgaccac cgcgtccacc
     4741 acccgggcgt actcgccctc gttgttcacg gtgactgggt agccggtcac gtactcaacg
     4801 tcggcgctca tgccgtgcgc ctgcgcgatg ccctgggcca gccgcgtgat ccgttcgccg
     4861 atcgcctcat ggttggccgc cgagaacgtg cgcaccgtgg attcgaggcg ggcgtcgtcg
     4921 ggaatgatgt tgtccttgct tcccgcggcg atcttgccga cggtcaccac caccgggtcg
     4981 aacgcatcga actgcctggt caccatggtc tgcagggcga tcgcgatctc gcacgccacc
     5041 ggcaccgggt cctgggcgtg atgcggctgg gagccgtgcc caccccgtcc gcgcaccgtg
     5101 atgtagcact ggtcggcggc cgccatcagc acgcccggac gtcccgacca caccccgaac
     5161 ggatgctcgg acgacaccac gtgcagcccg taggcggcgt cggcccggcg tcccgccgcg
     5221 tccagcacgc cctcggcgat catcggaccc gccccgcccg gtccctcctc ccccggctgg
     5281 aacatgaaca ccacgtctcc cggcaactgg tcgcgcaact cgtgcagaat cttcgccgcg
     5341 ccgacgagcc cggccacatg caggtcgtgg ccgcaggcgt gcatggtgcc gttggtggcc
     5401 gcgaactgct gtccggtcgc ctcgatcacg ggcagcccgt ccatgtcgcc gcgcagcaac
     5461 accaccggac gacgtcccgt cgtttcggcc cgtccgcgca gcaccgcgac cacgctgctc
     5521 agcgccgcac cggtggtgat ctcaaggtcg aggccctcca gcgccgcgag cacccgtgcc
     5581 tgcgtgcgcg gcaggtcgtt gccgagctcg ggttcagcgt ggatcgcccg gcgcagcgtg
     5641 cgcaactcgg ggtgcagacg ggcagcgatg gcggtggtgg cggtcattgg ctcagcctgc
     5701 cagagtccaa tgcgccggtg ggtcagtcgc gcagtgcgcg ccccagcgca gccaacctag
     5761 cctcgacggc gctcagcctg gcctccactt cggcgggcag tgccgcaccc gcaccgacgc
     5821 ctcctcccgc acccgacccg gacgccgttg ccgccgccgt cctccctcgc ccgaaccgcc
     5881 accagccgac accgacggtg atcgccagcg cggccgtcat caccgcgaag cgaggccact
     5941 ggttcgcggt cgacatgccc aggccgacgg catagatcat cgtgaacagc ccgccgagca
     6001 gcgccccgtt ggccagcacc gcccctgcct cggcgatggc cagcgacaac agcatcacca
     6061 gcgtggcgca gctcagcagc acgatcgagg taatcagccg ccactgcgaa tagtccggct
     6121 gactaccggt gacgtactcg ggctccggat agaaggtatt gacgccgatg ccgacgaagg
     6181 ccgtcacgac cagcccgaga aacacgctga agatcacctg gagcaccgac tggctcaact
     6241 cacgttgcgc attcatggcg tcaagcgtgc catcacgcag tccacgaaca gccgctccgc
     6301 accgggcgcc aggtcgagaa agtagctggg ctcgaacagt tccaactcca tcaacaacgg
     6361 acggtcctgc gcgtcgcgcg cgatgtcgac ccgcgcgtac agcagcgggc acacgtgcgc
     6421 caggccgcgg tcggtgaaga tgcgctccac gacggccagc gcctgccgcg ccagtgcgcg
     6481 ctcgtcgtcc ggggcgtgcg ccttcacgat cgattcctgg tactgctcac cgcgcagacc
     6541 gccgcccagg gccagcagcg gccccttgcc gatcgcgtgc acgaactcgt caccgaagta
     6601 gatcagcgag cgttcgccca cccggcccac ggattcgatc gccggctgga tcatcaccac
     6661 cttgcccgcg gccagaatct gctcggccag tcgcaccccg gccggatcgc cggcctcgaa
     6721 caaccccgtg tccgcggacg cggccgagac gttgggcttg agcaccaccc gtccggacgc
     6781 ctcggccagg gctgcgcgca cctcgtcgac gtgggcacag taggtggtcg ggcaggccgg
     6841 cacgcccagg gcggtcagtt cgcccaggta gcgcttgtcc atgttccagc ggatcacctc
     6901 gggcgggttc accacctcgg cgacggcctc gacccgggcc agccagtcga gaaactcgtc
     6961 gtgccggcct gcgtagtccc acggagactt gatcaccacc aggtcgcacg cggcgtagtc
     7021 ggcggtctgg tccaaccaat cgatggcgcg cacggtgaca ccgcgccggg cgagttcgtc
     7081 gccgatcagc gtgatctcgc gatcggcgtc caccgggctg gtcagggcgt attggctggt
     7141 gatcagggct accgtcggca tcaccccatc caactgcacc ggggcgctcc ggtcgccagg
     7201 tcgtcctcac cctggacgaa acccgtcccg gccgctccac gatggtcacg tgagcacggt
     7261 ggaaacctcc gactcaccat ggacgaactt cgtcccggcc gctccacgat ggtcacgtga
     7321 gcacggtgga acccaattca cctggacgaa acccgtcccg gccgatccac gatggtcacg
     7381 tgagcacggt ggaacccaat tcacctggac gaaacccgtc ccgaccgcgc catgatggtc
     7441 acgtgagcac ggcgaacccc tccgacgacc tcggcacggt gctgcgctgg caggccgccg
     7501 gcggcgagat cgaggtgctc agctgggggc ctccggtggt ggtgtcgctg tgtacgtgcg
     7561 acggcgggca gcagatgcag cgcctgacct ccgacgcgga cgacctgctg gcccacctgc
     7621 gcagtcggta gccgaccgca ccgcctcggg cctgcccacg ccgccgaacg ctcggtctcg
     7681 atcaggtgac gatctcggcg gtgggcacgt aattccttgt caccgcccaa agctgccgct
     7741 actgtgcggc catgtcagag cctcttgtcg tgatcgagaa cgtcaacaag cacttcggcg
     7801 acctgcatgt gctcaagaac atcaatctct cgatcgacaa gggcgaggtc gtcattctga
     7861 tcggcccttc gggctcaggt aagtccaccc tctgccgcac gatcaaccgg ctcgaaacct
     7921 acgagtcggg ttcgatcacg atcgacggca agaccctgcc ggatgagggc aaagagctcg
     7981 cgaacctgcg cgccgacgtc ggcatggtgt tccaatcgtt caacctgttc gcccacaaga
     8041 ccattctcga gaacatcacg ttgggcccga tcaaggtacg caagacgccg aaggccgagg
     8101 ccgagaaaaa ggcaatggaa ccgctcaccc gggtgggggt cgaggcgcag gccgagaagt
     8161 acccggcgca actctcgggc ggccagcagc agcgggtggc gatcgcccgc tcgctggcga
     8221 tgaaccccaa ggtgatgctg ttcgatgagc ccacctcggc gctggacccc gagatggtca
     8281 acgaggtgct ggacgtcatg gtgcagctcg ccaaagaggg catgacgatg atcgtggtca
     8341 cccatgagat ggggtttgcc cgcaaggcgg ccgacaaggt ggtcttcctg gccgacgggc
     8401 agatcgtcga aaccaacacc cccaccgaat tcttcgccaa cccgcaatcg ggtcgggcga
     8461 aggacttcct gtccaagatc ctgacccact gatccaaccg tccaacgaag ccgcagtaga
     8521 agaaggaacc gcaacctaac aaggagtccg tccatgtcac taaagttgaa gctcggcgcc
     8581 gcactcgcgg ccgcggctct cgctctctcc atgtccgcgt gtggtagcgg cgccggcagc
     8641 accaacctgc aggcgctcaa gatcggcatc aagttcgacc agcccggcct gggcctcaag
     8701 gacggtgaca agtacagcgg cttcgatgtg gacgtcgcca cctacatcgc cggcaagctc
     8761 ggcaccaccg ccgacaagat cacctggatc caggcgccgt ccgcgcagcg cgagacgctg
     8821 atcgagagcg ggcaggtcga cctggtggtg gccacctact ccatcaccga cgcgcgcaag
     8881 gagaaagtga gcttcgccgg accgtacttc atcgccggac aaagcctgct gatccgtgcg
     8941 gacgactcct cgatcaccgg ggtcgactca ctgtcgggca agaagctgtg ttcggtcacc
     9001 ggctccacct cggcgcagaa gatcaaggac aaggtgcccg gggtgaacct gcaagagttc
     9061 ggcggctact ccgactgcgt caccgcactc aatgccaaga ccatcgacgc gctgaccacc
     9121 gacgacacga ttctggcggg cttcgccgcc cagccggcca acgccggcaa actgaagctg
     9181 gtc
//
LOCUS       DAOSUG010000045         9176 bp    DNA     linear   ENV 10-JUN-2024
DEFINITION  MAG TPA_asm: Micropruina sp. isolate AMR_MDS_2195
            NODE_4027_length_9176_cov_4.354347, whole genome shotgun sequence.
ACCESSION   DAOSUG010000045 DAOSUG010000000
VERSION     DAOSUG010000045.1
DBLINK      BioProject: PRJNA925477
            BioSample: SAMN32864262
            Sequence Read Archive: SRR4236673
KEYWORDS    WGS; ENV; Metagenome Assembled Genome; MAG; Third Party Data; TPA;
            TPA:assembly.
SOURCE      Micropruina sp. (wastewater metagenome)
  ORGANISM  Micropruina sp.
            Bacteria; Bacillati; Actinomycetota; Actinomycetes;
            Propionibacteriales; Nocardioidaceae; Micropruina.
REFERENCE   1  (bases 1 to 9176)
  AUTHORS   Abdulkadir,N., Saraiva,J.P., Zhang,J., Stolte,S., Gillor,O.,
            Harms,H. and Rocha,U.
  TITLE     Genome-centric analyses of 165 metagenomes show that mobile genetic
            elements are crucial for the transmission of antimicrobial
            resistance genes to pathogens in activated sludge and wastewater
  JOURNAL   Microbiol Spectr 12 (3), e0291823 (2024)
   PUBMED   38289113
REFERENCE   2  (bases 1 to 9176)
  AUTHORS   Abdulkadir,N.
  TITLE     Direct Submission
  JOURNAL   Submitted (24-MAR-2023) Environmental Microbiology, Helmholtz
            Center for Environmental Research -UFZ, Permoserstr 15, Leipzig
            04318, Germany
COMMENT     The annotation was added by the NCBI Prokaryotic Genome Annotation
            Pipeline (PGAP). Information about PGAP can be found here:
            https://www.ncbi.nlm.nih.gov/genome/annotation_prok/
            
            ##Genome-Assembly-Data-START##
            Assembly Method        :: SPAdes v. 3.11.1
            Genome Representation  :: Full
            Expected Final Version :: Yes
            Genome Coverage        :: Not Applicable
            Sequencing Technology  :: Illumina MiSeq
            ##Genome-Assembly-Data-END##
            
            ##Genome-Annotation-Data-START##
            Annotation Provider               :: NCBI
            Annotation Date                   :: 05/04/2023 04:57:10
            Annotation Pipeline               :: NCBI Prokaryotic Genome
                                                 Annotation Pipeline (PGAP)
            Annotation Method                 :: Best-placed reference protein
                                                 set; GeneMarkS-2+
            Annotation Software revision      :: 6.5
            Features Annotated                :: Gene; CDS; rRNA; tRNA; ncRNA
            Genes (total)                     :: 2,776
            CDSs (total)                      :: 2,750
            Genes (coding)                    :: 2,714
            CDSs (with protein)               :: 2,714
            Genes (RNA)                       :: 26
            tRNAs                             :: 24
            ncRNAs                            :: 2
            Pseudo Genes (total)              :: 36
            CDSs (without protein)            :: 36
            Pseudo Genes (ambiguous residues) :: 0 of 36
            Pseudo Genes (frameshifted)       :: 2 of 36
            Pseudo Genes (incomplete)         :: 32 of 36
            Pseudo Genes (internal stop)      :: 2 of 36
            ##Genome-Annotation-Data-END##
FEATURES             Location/Qualifiers
     source          1..9176
                     /organism="Micropruina sp."
                     /mol_type="genomic DNA"
                     /submitter_seqid="NODE_4027_length_9176_cov_4.354347"
                     /isolate="AMR_MDS_2195"
                     /isolation_source="Activated sludge of municipal
                     wastewater treatment plants"
                     /db_xref="taxon:2737536"
                     /environmental_sample
                     /geo_loc_name="Canada"
                     /lat_lon="49.25 N 123.1 W"
                     /collection_date="2015-06-16"
                     /metagenome_source="wastewater metagenome"
                     /note="metagenomic"
     gene            complement(<1..210)
                     /locus_tag="PLF56_02575"
     CDS             complement(<1..210)
                     /locus_tag="PLF56_02575"
                     /inference="COORDINATES: ab initio
                     prediction:GeneMarkS-2+"
                     /note="Derived by automated computational analysis using
                     gene prediction method: GeneMarkS-2+."
                     /codon_start=1
                     /transl_table=11
                     /product="hypothetical protein"
                     /protein_id="HOQ52480.1"
                     /translation="MSIPRFDSIELGDDAPPSDSAQQWEDLIDQVPSYSKGWQTPEHI
                     LVPPVYDDAAYAGLDFLNTYPGIPPF"
     gene            complement(207..2090)
                     /gene="mutA"
                     /locus_tag="PLF56_02580"
     CDS             complement(207..2090)
                     /gene="mutA"
                     /locus_tag="PLF56_02580"
                     /EC_number="5.4.99.2"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_013160682.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_function: GO:0004494 - methylmalonyl-CoA mutase
                     activity [Evidence IEA];
                     GO_process: GO:0008152 - metabolic process [Evidence IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="methylmalonyl-CoA mutase small subunit"
                     /protein_id="HOQ52481.1"
                     /translation="MTETPLTLAGDFTAPSRQDWEAEVLKVLNRRRPEGKELSIEQAM
                     KRLTTITVDDLVIDPLYTRATDTAADHDLGHPGVTPFTRGTSLRTGAIHAWDVTQLHE
                     DPDVAFSNAAVLADLTKGATSLWLRVDPDAIAAADLGAVLAGVLPELARLRVSSHTDQ
                     FAAAAALADFWRAGGKAAEVGGSLGIDVLGHAARTGEAPDFAAQREWVSTSLTEFPKA
                     RALAVDTLVYDDAGAGDVDVLAFAIATGVEHLRDLEQAGIGAEQVFAQIAFRVPINAD
                     QFMTIARLRALRRLWARVGEVSGVRSGKRGAIQLAVTSGRMVTRDDPWVNLLRGTIAT
                     FGGAIGGAESITVLPHDTAWGLPTDFSRRMARNIQLLAAEESNLGRVADPAGGSWYVE
                     SLTDQLAERAWKRFQEIEAGGGMATVLADGGVAAAIERTVAARAVRLANRRQPITGVS
                     MFPNANEQPLEVRPRPAAPSYGGLKPRRDSEVFEALRDRVNAAAEKPKVFMACLGARR
                     DFGPREQFTSNVLLVAGFDHPSSEGGSPDEIVQQVEAAGANVVVLCSSAKVYAAQAIE
                     VAKALKAAGVGTVLIAGRRTETGSDEAAGLIDAEIFDGMDVAGFLSGILDQLGLAQAG
                     AQQ"
     gene            complement(2207..3427)
                     /locus_tag="PLF56_02585"
     CDS             complement(2207..3427)
                     /locus_tag="PLF56_02585"
                     /EC_number="2.7.1.-"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_015582242.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_function: GO:0005524 - ATP binding [Evidence IEA];
                     GO_process: GO:0016310 - phosphorylation [Evidence IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="aminoglycoside phosphotransferase family
                     protein"
                     /protein_id="HOQ52482.1"
                     /translation="MTDQDPDGSRLLTSSEVGELLGAAVEHAGGRLVSWALDHVDANP
                     QQSTTATYSATVAWPYGERDELLGVSARASGPAATDSRAEIFADGEREVAVWIYPADP
                     DLPGLARAAYTEQMAGILTDANVLGRPVGPAEVSLRMIGYRPRRRAVLKVEVGDPPVV
                     LYCKVLRERVFHDVVRRHELLLDAGVPAPLIAATTPDALLVLHTLPGRSLAKAVFDES
                     DPCTAEQLIQLLDAMPMSITRLERRPPWSDAVQHYAQMVSAALPAATDKLARLVQQVT
                     DGLAHIPLGNEPTHGDFHEGQVHVAGGRIVGMLDVDTVGPGRRADDLACLIAHLSTIQ
                     RMSPAQEARVHRLVRTWVPVFDTRVDPRELRLRAAAVIISLATGPFRGQEPDWEWETM
                     RMIASAEALVRQVS"
     gene            complement(3424..4758)
                     /locus_tag="PLF56_02590"
     CDS             complement(3424..4758)
                     /locus_tag="PLF56_02590"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_015071125.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_component: GO:0016020 - membrane [Evidence IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="hemolysin family protein"
                     /protein_id="HOQ52483.1"
                     /translation="MNPVLSNIALILLFIVIGGVFAAAEMALVSLRESQVKQLSTRGT
                     RGKTVAALAANPNRFLSAVQIGVTLSGFLSAAFGGATLEEPLSQLLIGWGVPEGVAPP
                     LSLVLITVIISYFSIVVGELTAKRLAMQSAESFAMALAPLVNSIATLARPVIWFLGVS
                     TNALVRVFGGDPKAGREEVTDEELRAMVTSSSTLGDEERQIVDEVFAAGQVSLREAMV
                     PRTEVDFITGDMPAHKVIRSFHDSPHSRYPVIGEDVDDVLGFVHVRDLFDLDPAARNA
                     PVRQLVRPVVSLPHTMKILRALSELRAANAHLSIVRDEYGGTAGIVTMEDLVEELVGD
                     ISDEFDQPVVESPASPTRAELDGLETIEEFGETIGYVLPEAPYDTVAGFVMAHLGHLP
                     EFNDTVEVVLDPVPGSDNEPERFCFTVTELDGRRAARLRLERLGAVPGSDQT"
     gene            4816..6066
                     /locus_tag="PLF56_02595"
     CDS             4816..6066
                     /locus_tag="PLF56_02595"
                     /inference="COORDINATES: protein motif:HMM:NF016357.2"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_component: GO:0016020 - membrane [Evidence IEA];
                     GO_function: GO:0047355 - CDP-glycerol
                     glycerophosphotransferase activity [Evidence IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="CDP-glycerol glycerophosphotransferase family
                     protein"
                     /protein_id="HOQ52484.1"
                     /translation="MSATVPRPFTFAKGNAAKLAALPRYASGSVRTRFRSRDPQVWAF
                     GSAFGLADGALALLRAARRLQPSLRLVWLVGTEQQAVQARSLGIEWVAKDSSAGFDTT
                     AGAGVVAITHGFGDVNRFALGGATIAQLWHGSPLKKLHADSSAALALGPLSGVPAART
                     LMRQAYRRGTRRISVLPVAADTFVPWMCSAFDLSPQQVRVLGEPRTDVLFAGPPAGRR
                     AAARDLLQRAVPGLGGRRVVLYAPTWRDGEPDPGIPTQRQWRRIDAWCEATDSVLLVR
                     PHPLGVGSYTHNSRYVHLVPPTLLPESMPLLWGVDTLITDYSSMLFDFAVTGGPMVFL
                     APDLEHYAATRGLYPDYAQITGGRWRTDWDGVLDRLERLDAEPSALERARAHTARIAA
                     LAHRHTDGRSAHRVASHLLELTGS"
     gene            complement(6081..7118)
                     /locus_tag="PLF56_02600"
     CDS             complement(6081..7118)
                     /locus_tag="PLF56_02600"
                     /EC_number="2.4.-.-"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_014846028.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_function: GO:0016757 - glycosyltransferase activity
                     [Evidence IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="glycosyltransferase family 2 protein"
                     /protein_id="HOQ52485.1"
                     /translation="MSEFPPVSYVMPVLNEQGYLADAVAAVLAQSYPAEQELVIALGA
                     STDDTTAIAHELAETDARITLVDNPVNDIPVGLNLAIAASRHPIVVRVDAHAELPADY
                     TRTMVGLLNETGAVNVGGVMHAKGRTPFQAAVARAYNSPWGLGGGQYHGAKQAGPSDS
                     AYLGVFRREVLDEVGGYDDTLRRAEDWELNSRILAAGHLIWYTPDVAVTYWPRATPAS
                     LRRQMYATGVWRGHLVREQGRTPLKYLPPPLVTLALSVSALVAVVQRNRVLPRRAARL
                     ASVVHAAPMLYAAGLGWVSVRSLGAGNAREWLLNVAVLGIMHLSWGAGFLRGLFAGAH
                     TTVDTSRLANR"
     gene            complement(7230..8390)
                     /locus_tag="PLF56_02605"
     CDS             complement(7230..8390)
                     /locus_tag="PLF56_02605"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_012919896.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_function: GO:0005524 - ATP binding [Evidence IEA];
                     GO_function: GO:0016887 - ATP hydrolysis activity
                     [Evidence IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="AAA family ATPase"
                     /protein_id="HOQ52486.1"
                     /translation="MLTTLAVHGYRSLRDVVLPLAPLTVITGANGSGKSNLYRAFHLL
                     ADTANGRLIGALATAGGLSSVLWAGPEQITGAMRRGEVPIQGTGSRKQPISLQLGFTG
                     DDFGYLIDIGLPVPAQTLFSRDPVIKREVIWAGTQLRPAATLLERTTRGVRVAESSTR
                     VDLPIGLPPWASVLRELVDPVHRPELAAVCEVVRGWRFYDSFRVDADAPARLPQVGTR
                     TEVLAGDGHDLAPAAATILESAWAGPLQRAVADAFDGSRLTVTDYDGRFEVALTQTGL
                     LRPLTARELSDGTLRYLLLAVALFSPRPPGLMVLNEPETSLHPDVLPALARLMVAAGE
                     RCQLVVVTHSAPLVDALVAAGARHHELIKPHGETEVADQGLLSRPTWRWGTR"
     gene            8667..8903
                     /locus_tag="PLF56_02610"
     CDS             8667..8903
                     /locus_tag="PLF56_02610"
                     /inference="COORDINATES: ab initio
                     prediction:GeneMarkS-2+"
                     /note="Derived by automated computational analysis using
                     gene prediction method: GeneMarkS-2+."
                     /codon_start=1
                     /transl_table=11
                     /product="hypothetical protein"
                     /protein_id="HOQ52487.1"
                     /translation="MRSQVRAAAVRTVSLLALSVAVYRAVTTYADVAVRLLRSLGLRQ
                     RQLEPWRSLGTLHQWPPTPGFHKLNQRTTEIHDW"
     gene            8943..>9176
                     /locus_tag="PLF56_02615"
     CDS             8943..>9176
                     /locus_tag="PLF56_02615"
                     /EC_number="1.1.1.42"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_011757006.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_function: GO:0004450 - isocitrate dehydrogenase (NADP+)
                     activity [Evidence IEA];
                     GO_process: GO:0006099 - tricarboxylic acid cycle
                     [Evidence IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="NADP-dependent isocitrate dehydrogenase"
                     /protein_id="HOQ52488.1"
                     /translation="MSSIIYTHTDEAPLLATYSFLPIVQAYAAAAGVEVQTRDISLAG
                     RIIAQFPEHLSEQQRLPDALVELGALAKTPEANI"
BASE COUNT         1383 a         3225 c         3208 g         1360 t
ORIGIN      
        1 gaacggcgga atgcccgggt aggtgttcag aaagtccagg ccggcgtagg cggcgtcgtc
       61 gtagaccggc ggcaccagaa tgtgctcggg tgtctgccag cccttgctgt aggacggcac
      121 ctggtcgatc aggtcctccc actgctgggc ggagtcggac gggggcgcgt catcgcccag
      181 ttcgatgctg tcgaaacgag gaatgctcac tgttgcgctc ctgcctgggc gaggcccagt
      241 tggtcgagaa tgccggagag gaagccggcg acgtccatgc cgtcgaagat ctctgcgtcg
      301 atcaggccgg cggcctcatc ggagccggtc tcggtgcggc gtccggcgat cagcacggtg
      361 cccacaccag cggccttcag ggccttggcc acctcgatcg cctgcgccgc gtacaccttg
      421 gccgacgagc acagcaccac gacgttcgcg ccggccgctt cgacctgctg gacgatctcg
      481 tcgggagatc cgccctcgct gctggggtgg tcgaacccgg ccaccagcag cacgttcgag
      541 gtgaactgct cacgcgggcc gaaatcgcgc cgggcaccca ggcaggccat gaagaccttg
      601 ggcttctcgg cggcggcgtt cacccggtcg cgcagcgcct cgaagacctc gctgtcgcgg
      661 cgcggcttga ggccgccgta gctcggggct gccggtcgcg gacgaacctc gagcggttgc
      721 tcgttggcgt tggggaacat cgacacgccg gtgatcggct ggcgccggtt ggcaagccgg
      781 acggcgcggg ccgccaccgt acgctcgatc gctgcggcga cgccgccgtc ggcgagcacc
      841 gtggccatgc cgccgcctgc ctcgatctct tggaaccgct tccaggcccg ttcggcgagc
      901 tggtcggtga ggctctcgac gtaccacgag ccgcccgccg gatcggcgac ccggcccagg
      961 ttcgactcct cggcggccag caactgaatg ttgcgggcca tccgacggga gaagtcggtg
     1021 ggcaggcccc aggcggtgtc gtgcggcagc accgtgatcg attcggcgcc gccgatggcg
     1081 ccgccgaagg tggcgatggt gccgcgcagc aggttgaccc acgggtcgtc ccgggtcacc
     1141 atgcgtccgg acgtgacggc cagctgaatg gcgccgcgct tgccggagcg gacgccgctg
     1201 acctcgccga cccgggccca cagccggcgc agtgcgcgca gccgggcgat ggtcatgaac
     1261 tggtcggcgt tgatcggcac ccggaaggcg atttgggcga agacctgttc ggcgccgatg
     1321 ccggcctgtt cgagatcgcg caggtgctcg acgccggtgg cgatcgcgaa ggcgagcacg
     1381 tccacgtcgc cggcgcccgc atcgtcgtag acgagggtgt cgaccgcgag cgcgcgggcc
     1441 ttgggaaact cggtgagcga ggtactgacc cattcgcgct gcgcggcgaa gtcgggggcc
     1501 tcgccggtac gggccgcatg acccagcacg tcgatgccga ggctgccacc gacctcggcg
     1561 gccttgccgc cggcccgcca gaagtcggcg agggccgcgg cggcggcgaa ctgatcggtg
     1621 tggctgctga cccggagccg ggcgagttcc ggcagcacgc cggccagcac ggcgccgaga
     1681 tcggcggcgg cgatggcgtc cggatcgacc cgtagccaca gcgaggtggc acccttggtc
     1741 aggtcggcga gcacggccgc gttgctgaac gcgacgtcgg ggtcttcgtg aagctgggtg
     1801 acgtcccagg cgtgaatggc tccggtgcgc agcgaggtgc cccgggtgaa cggggtcacg
     1861 cccgggtgcc ccaggtcgtg gtcggcggcg gtgtcggtgg cgcgggtgta gagcgggtcg
     1921 atgacgaggt cgtccacggt gatcgtggtg agtcgcttca tcgcctgctc gatggagagt
     1981 tccttgccct cgggacggcg ccggttgagc accttgagga cctcggcctc ccagtcctgc
     2041 cggctcggcg cggtgaagtc gccggccagg gtgagcgggg tttcggtcat gatcgcagcc
     2101 taggactctg accctctcac cgctgcccaa cgggcgaatg acttgggctc tggaagcatg
     2161 aatctcgacc tgcgagacta cccgcggccc gggacgccgg gccgggtcaa ctgacctgcc
     2221 gcaccagcgc ctcggccgag gcgatcatgc gcatggtctc ccattcccaa tccggctcct
     2281 ggccacggaa cggcccggtg gccagcgaaa tgatcacagc ggcggcccgc agccgcagtt
     2341 cgcgaggatc gacccgggtg tcgaacaccg gcacccaggt acggaccagc cggtgcaccc
     2401 gcgcctcctg cgcaggactc atgcgctgaa tggtcgacag gtgcgcgatc aggcaggcca
     2461 ggtcgtccgc ccggcgtccg gggccgaccg tgtcgacatc gagcatgccg acgatgcggc
     2521 ccccggccac atgcacctgg ccctcgtgga agtcgccgtg ggtgggttcg ttgcccagtg
     2581 gaatgtgggc cagcccgtcg gtcacctgct gcaccagccg ggccagcttg tcggtcgccg
     2641 ccgggagcgc cgccgacacc atctgggcgt agtgctgcac cgcgtccgac cagggtggac
     2701 gccgctccag ccgggtgatg ctcatcggca tggcgtccag caattggatg agttgctcgg
     2761 ccgtgcacgg gtcagactcg tcgaagaccg ccttggccag cgaccgtccg ggcagggtgt
     2821 gcagcaccag aagggcgtcc ggggtggtcg cggcgatgag cggggccggc accccggcgt
     2881 ccagcagcaa ctcgtgccgc ctgaccacgt cgtggaacac ccgctcgcgc agcaccttgc
     2941 agtacagcac caccggcgga tcgccgacct cgaccttcaa cacggcccgg cgccgcggcc
     3001 ggtacccgat catgcgcagc gacacctcgg cgggcccgac gggccgaccg agcacgttcg
     3061 cgtcggtgag aatgccggcc atctgctcgg tgtaggcggc ccgggccagc cccggcaggt
     3121 cgggatcggc cgggtagatc cacacggcca cctcgcgctc gccgtcggcg aagatctcgg
     3181 cgcggctgtc ggttgccgca ggcccggacg cccgcgcgct cacgcccagc agttcgtcgc
     3241 gctcgccgta cggccaggcc accgttgcgg agtaggtggc ggtggtggac tgctgcgggt
     3301 tggcgtccac atggtccagc gcccaactca ccagccgtcc ccctgcatgt tcgacggccg
     3361 cgccgagcaa ctcgcccacc tcggacgagg tcagcaggcg cgaaccgtcc gggtcctgat
     3421 cggtcatgtc tggtccgatc ccggcacggc gcccagacgc tccagccgca accgggccgc
     3481 gcgccgtccg tccagttcgg tgacggtgaa gcagaaccgc tcgggttcgt tgtcgctgcc
     3541 cggcaccggg tcgagcacca cctcgacggt gtcgttgaac tccggcaggt gccccagatg
     3601 ggccatcacg aagccggcga cggtgtcgta gggggcctcc ggcagcacgt acccgatggt
     3661 ttcgccgaac tcctcaatgg tttcgaggcc gtccagctcg gcccgggtgg gcgaggccgg
     3721 cgattcgacg accggttggt cgaactcgtc ggagatgtcg ccgaccagct cttcgaccag
     3781 atcctccatc gtcacgatgc cggcggtgcc gccgtactcg tcgcgcacga tcgacagatg
     3841 cgcgttcgcg gcccgcagtt cggacagtgc tcgcaggatc ttcatggtgt gcggcaacga
     3901 gaccaccgga cgcaccagct gccgcaccgg cgcgttgcgg gcggccggat cgaggtcgaa
     3961 caggtcgcgc acatgcacga agcccagcac gtcgtcgacg tcctcgccga tcaccgggta
     4021 gcggctgtgc ggtgagtcat ggaacgagcg gatcaccttg tgggcgggca tgtcgccggt
     4081 gatgaagtcg acctcggtgc gcggcaccat cgcttcgcgc aggctgacct ggccggcggc
     4141 gaacacctcg tccacgatct ggcgctcctc atcgcccaac gtggacgacg aggtgaccat
     4201 ggcgcgcaac tcttcgtcgg tgacctcttc ccggccggcc ttcgggtcgc cgccgaacac
     4261 ccgcaccagg gcgttggtcg agacccccag aaaccagatc accggacggg ccagggtggc
     4321 gatggagttg accagcggcg ccagcgccat ggcgaagctc tcggcgctct gcatggccag
     4381 gcgctttgcg gtgagttcgc ccaccacgat cgagaagtaa ctgatgatca ccgtgatcag
     4441 caccagcgag agcggcgggg ctacaccctc gggcactccc cacccgatca gcaactgcga
     4501 cagcggctct tcgagcgtcg caccgccgaa ggcggcggaa aggaaccccg acaacgtgac
     4561 gccgatctgc acggcggaaa ggaaccggtt ggggttcgcg gccagcgcag cgacggtctt
     4621 gccccgggta ccgcgggtcg acagctgctt gacctggctt tcgcgcaacg acaccagggc
     4681 catctcggcg gcggcgaaca ccccgccgat gacgatgaac agcaggatca gggcaatgtt
     4741 gctgagaaca gggttcatcg gctcgcctcg atcaccggca tagtttatag atcagggctc
     4801 ggtaaggtga cggcgatgtc cgccaccgtt ccgcgcccgt tcacgttcgc gaagggcaat
     4861 gccgccaaac tggccgcgct gccccgctac gcgtccggca gcgtgcgcac gcggttccgc
     4921 agccgtgacc cacaggtgtg ggccttcggc agcgccttcg gtttggccga cggtgcgctg
     4981 gcactgctgc gggccgcccg gcgtctgcaa ccgtcgctgc ggctggtgtg gctggtcggc
     5041 accgaacagc aggccgtgca ggcccgctcg ctcggcatcg aatgggtggc gaaggacagc
     5101 tccgccggct tcgacaccac cgcgggtgcc ggcgtcgtcg cgatcacgca cgggttcggc
     5161 gacgtgaacc gcttcgcgct cggcggagcg accattgcgc agctgtggca cgggtcaccg
     5221 ctgaagaagc tgcacgccga ctcgtccgcc gcgctcgcgc tgggcccctt gagcggggtg
     5281 ccggcggcac gcacattgat gcgccaggcc taccgtcgcg gcacccggcg gatcagcgtg
     5341 ctgccggtgg ccgccgacac gttcgttccg tggatgtgtt cggccttcga cctgagcccg
     5401 cagcaggtgc gggtgctggg cgagccgcgc accgacgtgc tgttcgccgg ccccccggcc
     5461 ggacgccggg ctgccgcccg cgacctgctg cagcgcgccg tcccgggtct gggtggacgg
     5521 cgggtcgtgc tgtatgcgcc cacgtggcgc gacggcgaac ctgaccccgg catacccacg
     5581 cagcgtcagt ggcgccggat cgatgcctgg tgcgaggcaa ccgacagcgt tctgctggtc
     5641 cgtccgcacc cgctgggggt gggctcctac acccacaact cgcgttacgt gcacctggtg
     5701 ccgcccaccc tgctacccga atcaatgccc ttgctgtggg gcgtggacac gctgatcacc
     5761 gactactcct cgatgctgtt cgacttcgcc gtcaccggcg gcccgatggt gtttctggcg
     5821 cccgatctgg agcactatgc ggcgactcgc gggctgtacc ccgactatgc ccagatcacc
     5881 ggcgggcgct ggcgcaccga ctgggacggg gtgctcgacc ggctcgagcg actcgacgcc
     5941 gaaccgtccg ccctggagcg cgcccgggcc cacaccgcac ggatcgccgc cctggcccac
     6001 cggcacaccg acggacgcag cgcacaccgg gtcgcgagcc acctgttgga gctcaccggg
     6061 tcgtgatccg agggccggcg ctagcggtta gcgaggcggg acgtgtcgac ggtggtgtgg
     6121 gcacccgcga acagtccgcg cagaaagccg gccccccacg acaggtgcat gatgccgagc
     6181 accgccacgt tcagcagcca ttcgcgggcg ttgccggcac ccagtgaacg aaccgagacc
     6241 caacccaacc cggcggcgta cagcatcggc gccgcgtgga cgacgctcgc cagtcgggcg
     6301 gcgcggcgag gcagcacccg gttgcgctgc acgaccgcca ccagggccga aacgctgagc
     6361 gccaaggtga ccagcggcgg cggcaggtac ttcaggggtg tgcggccctg ctcgcgcacc
     6421 aggtggcccc gccacacgcc ggtcgcgtac atctgccggc gcaggctggc cggggtggcc
     6481 ctgggccagt aggtgacggc gacgtcgggg gtgtaccaga tcaggtgacc cgcggcgagt
     6541 atgcgcgagt tgagctccca gtcctcggcc cggcgcagcg tgtcgtcgta gcctcccacc
     6601 tcgtcgagca cctcacggcg gaacaccccc aggtaagccg agtcggacgg cccggcctgc
     6661 ttggccccgt ggtactgccc gccgcccagg ccccagggcg agttgtaggc tcgcgcgacc
     6721 gccgcctgaa aaggcgtgcg ccccttggcg tgcatcacgc cgcccacgtt gaccgccccg
     6781 gtctcgttga gcaggccgac catggtgcgg gtgtagtcgg ccggcagttc tgcgtgcgcg
     6841 tccacccgga cgacgatggg gtgccggctt gccgctatcg ccagattgag cccgaccgga
     6901 atgtcgttga ccgggttgtc gaccagcgtg atgcgcgcgt cggtctcggc cagctcgtgg
     6961 gcgatcgcgg tggtgtcgtc ggtggacgca cccagggcga tcaccagctc ctgctcggcg
     7021 gggtagctct gtgcgagcac ggcggcgaca gcatcggcca ggtatccctg ctcgttcagc
     7081 accggcatca cgtacgaaac aggagggaac tcgctcattg gccgaggcta tcgcggcgga
     7141 cccgcagtga ccgtgcaccc ttcggtcgag tacggcctca caccttggcc ctcgggtcgg
     7201 cacgaaatcc acccgctgcg cgcagaacct catcgcgtac cccagcgcca ggtcggccgg
     7261 ctcaacaggc cctgatcggc cacctcggtt tcaccgtgtg gcttgatcag ctcgtggtgc
     7321 cgcgcgccgg cggcaaccag tgcatcgacc agaggtgcgg aatgggtgac caccaccaac
     7381 tggcaccgct cgcccgctgc gaccatcagc cgggccaggg ccggaagcac gtcggggtgc
     7441 agcgacgttt cgggctcgtt gagcaccatc aaccctggcg gacgaggact gaacagcgcc
     7501 accgccagca gcaggtaacg cagcgtgccg tccgagagtt cgcgagcggt cagcggacgc
     7561 agcaggccgg tttgggtcag cgcgacctcg aagcgtccgt cgtagtcggt gaccgtgagc
     7621 ctgctgccgt cgaacgcgtc ggcgaccgcg cgctgcagcg gtccggccca ggccgactcg
     7681 agaatggtcg ccgccgcggg cgccaggtcg tggccatcgc ccgccagcac ctcggtgcgg
     7741 gtgccgacct gaggcaggcg ggcgggtgcg tcggcatcga cccggaagct gtcgtagaag
     7801 cgccagccgc gcaccacctc gcagaccgcg gccagttcgg gccggtgaac agggtcgacg
     7861 agttcgcgca gcacgcttgc ccagggcggc aggccgatgg gcaggtccac ccgcgtggac
     7921 gactcggcca cccggacccc acgcgtcgtg cgctccagca gggtcgccgc cggacgaagc
     7981 tgcgttccgg cccagatcac ttcgcgcttg atcaccggat cgcggctgaa cagggtctgc
     8041 gccggcaccg gcaggccgat gtcgatcagg tagccgaagt cgtcgccggt gaaccccagc
     8101 tgcaggctga tcggctgctt gcgtgacccg gtgccctgga tcggcacttc gccgcgccgc
     8161 atcgcgccgg tgatctgctc gggccctgcc cacagcaccg aactcaaccc cccggcggtc
     8221 gcgagcgcgc cgatcagtcg gccgttcgcg gtgtcggcca gcaggtgaaa ggcgcggtac
     8281 aggttggact tgcccgaccc gttcgcgccg gtgatgacgg tcagcggtgc gagcggcagc
     8341 acgacgtcgc gcagcgagcg ataaccgtgc acggccaggg tggtgagcat ggcactgacg
     8401 ctagaacccg gcaccgacag ttccagcgct cgtgcgcacc gcctccatga cacgggtcgc
     8461 gccgtcccca tgaaacaggt cacgacattt caggtacgag gtaggactcg gcgggcgtcc
     8521 ccgcgtgagg cggacttgtt ccactcacgc tgcggctgtg cgccgattcg gcgcagcacg
     8581 catcaatccg gagctcggac gactcctgca cgtgacgccc ggtatcggga gggcccagga
     8641 cgttgacccg cgaggatcca cgaccgttgc gatcgcaggt gcgcgcagcg gccgtccgca
     8701 cggtctctct gctcgccctg tcggtggctg tctaccgggc ggtcaccacc tacgccgacg
     8761 tcgccgtgcg gctgctccgc tccctcggcc tgcgccagcg gcagctcgag ccgtggcgct
     8821 cgctcggcac cctccaccaa tggcccccca cccctgggtt ccacaagctc aaccaacgga
     8881 ccaccgagat ccacgattgg tagtctcctg acgtccacca cgttgaggtc cggaaggtca
     8941 cgatgagctc gatcatctac acgcacacag acgaggcgcc gctgctggcg acgtactcct
     9001 ttctgcccat cgtgcaggcg tacgcggccg cggcgggggt cgaggtgcag acccgcgaca
     9061 tttcgctggc cggacgcatc atcgcccagt tccccgaaca cctctccgag caacagcggc
     9121 tgccggacgc cctggtcgag ctcggcgcgc tggccaagac ccccgaggcg aacatc
//
LOCUS       DAOSUG010000046         9164 bp    DNA     linear   ENV 10-JUN-2024
DEFINITION  MAG TPA_asm: Micropruina sp. isolate AMR_MDS_2195
            NODE_4035_length_9164_cov_4.140850, whole genome shotgun sequence.
ACCESSION   DAOSUG010000046 DAOSUG010000000
VERSION     DAOSUG010000046.1
DBLINK      BioProject: PRJNA925477
            BioSample: SAMN32864262
            Sequence Read Archive: SRR4236673
KEYWORDS    WGS; ENV; Metagenome Assembled Genome; MAG; Third Party Data; TPA;
            TPA:assembly.
SOURCE      Micropruina sp. (wastewater metagenome)
  ORGANISM  Micropruina sp.
            Bacteria; Bacillati; Actinomycetota; Actinomycetes;
            Propionibacteriales; Nocardioidaceae; Micropruina.
REFERENCE   1  (bases 1 to 9164)
  AUTHORS   Abdulkadir,N., Saraiva,J.P., Zhang,J., Stolte,S., Gillor,O.,
            Harms,H. and Rocha,U.
  TITLE     Genome-centric analyses of 165 metagenomes show that mobile genetic
            elements are crucial for the transmission of antimicrobial
            resistance genes to pathogens in activated sludge and wastewater
  JOURNAL   Microbiol Spectr 12 (3), e0291823 (2024)
   PUBMED   38289113
REFERENCE   2  (bases 1 to 9164)
  AUTHORS   Abdulkadir,N.
  TITLE     Direct Submission
  JOURNAL   Submitted (24-MAR-2023) Environmental Microbiology, Helmholtz
            Center for Environmental Research -UFZ, Permoserstr 15, Leipzig
            04318, Germany
COMMENT     The annotation was added by the NCBI Prokaryotic Genome Annotation
            Pipeline (PGAP). Information about PGAP can be found here:
            https://www.ncbi.nlm.nih.gov/genome/annotation_prok/
            
            ##Genome-Assembly-Data-START##
            Assembly Method        :: SPAdes v. 3.11.1
            Genome Representation  :: Full
            Expected Final Version :: Yes
            Genome Coverage        :: Not Applicable
            Sequencing Technology  :: Illumina MiSeq
            ##Genome-Assembly-Data-END##
            
            ##Genome-Annotation-Data-START##
            Annotation Provider               :: NCBI
            Annotation Date                   :: 05/04/2023 04:57:10
            Annotation Pipeline               :: NCBI Prokaryotic Genome
                                                 Annotation Pipeline (PGAP)
            Annotation Method                 :: Best-placed reference protein
                                                 set; GeneMarkS-2+
            Annotation Software revision      :: 6.5
            Features Annotated                :: Gene; CDS; rRNA; tRNA; ncRNA
            Genes (total)                     :: 2,776
            CDSs (total)                      :: 2,750
            Genes (coding)                    :: 2,714
            CDSs (with protein)               :: 2,714
            Genes (RNA)                       :: 26
            tRNAs                             :: 24
            ncRNAs                            :: 2
            Pseudo Genes (total)              :: 36
            CDSs (without protein)            :: 36
            Pseudo Genes (ambiguous residues) :: 0 of 36
            Pseudo Genes (frameshifted)       :: 2 of 36
            Pseudo Genes (incomplete)         :: 32 of 36
            Pseudo Genes (internal stop)      :: 2 of 36
            ##Genome-Annotation-Data-END##
FEATURES             Location/Qualifiers
     source          1..9164
                     /organism="Micropruina sp."
                     /mol_type="genomic DNA"
                     /submitter_seqid="NODE_4035_length_9164_cov_4.140850"
                     /isolate="AMR_MDS_2195"
                     /isolation_source="Activated sludge of municipal
                     wastewater treatment plants"
                     /db_xref="taxon:2737536"
                     /environmental_sample
                     /geo_loc_name="Canada"
                     /lat_lon="49.25 N 123.1 W"
                     /collection_date="2015-06-16"
                     /metagenome_source="wastewater metagenome"
                     /note="metagenomic"
     gene            complement(1..749)
                     /gene="ccsB"
                     /locus_tag="PLF56_02620"
                     /pseudo
     CDS             complement(1..749)
                     /gene="ccsB"
                     /locus_tag="PLF56_02620"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_015583280.1"
                     /note="incomplete; partial in the middle of a contig;
                     missing N-terminus and C-terminus; Derived by automated
                     computational analysis using gene prediction method:
                     Protein Homology.
                     GO_function: GO:0020037 - heme binding [Evidence IEA];
                     GO_process: GO:0017004 - cytochrome complex assembly
                     [Evidence IEA]"
                     /pseudo
                     /codon_start=1
                     /transl_table=11
                     /product="c-type cytochrome biogenesis protein CcsB"
     gene            complement(1058..2917)
                     /locus_tag="PLF56_02625"
     CDS             complement(1058..2917)
                     /locus_tag="PLF56_02625"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_015583281.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_component: GO:0016020 - membrane [Evidence IEA];
                     GO_process: GO:0017004 - cytochrome complex assembly
                     [Evidence IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="cytochrome c biogenesis protein ResB"
                     /protein_id="HOQ52489.1"
                     /translation="MPTDKHLIDPFDQPEAEAAGEASAGASGESGREDGSSASGQEGG
                     RDASGAGPAPGDDASGASGRQVHGDASGAVSPGKAPTRRTTAATRPGGTLRASETLRW
                     LWTQLTSMRTALVLLFALALGAIPGSLLPQRPTSPVRVREFLTNNPQLGGFYDAIGLF
                     DVYASPWFAAIYLLLFVSLVGCIIPRIGVYLRGLRAQPPRTPRNLGRLPAHLDFAGAA
                     TPVLLDQAETLLRKRRFRVRRLPDSVSAERGYVRELGNLVFHVSLVFLLCGVAWGALF
                     GYKGTVALVEGQSFSNTLTQFDDFTSGGAFRASQLVPFTVKLDSFEAKFETGPVQRGA
                     ARVFKANTTTTMDGVSRQQTLEVNQPLVVGDSTVHLVGHGYAVDVTVKGADGKVAFEG
                     PVILLPQDGNFLSVGVVKAPYARPDRLAFEALFLPTAVDSANGGTSVFPDALNPLLLL
                     NAWHGAPKEATGVPENVYSLDTTGLTQYTKDGKPLRFTLAPGEVFDLPGGGSITFHDW
                     KRWVSLQVGSTPGLWLVFGSVLVAVAGLCLSLFVRPRRVWVKVSGDRVEVAGLDRTEG
                     RGGLDDELVLISDGLGLAPAPAAGAGSVASEPDAAEPPAGTTGGGTADDEGKS"
     gene            complement(2917..3627)
                     /locus_tag="PLF56_02630"
     CDS             complement(2917..3627)
                     /locus_tag="PLF56_02630"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_002545769.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_component: GO:0016020 - membrane [Evidence IEA];
                     GO_process: GO:0017004 - cytochrome complex assembly
                     [Evidence IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="cytochrome c biogenesis protein CcdA"
                     /protein_id="HOQ52490.1"
                     /translation="MLLAVPVALLAGLVSFFSPCVLPLLPGYLSYASGLSAAQLQAGE
                     SRGRVLAGTSLFVLGFAVVFVSGGALFGSLGTTLMQYRTTINVIGGVLAIALGLVFAG
                     WVPFASRTVRLNLRPKVGLAAAPLLGLVFALGWTPCIGPALQVVLTLALNEATALRGG
                     LLAFCYALGLGLPFLVAGLALDRFGGALGFVRRHHRALQVMGGVMMIAVGVLLLTGWW
                     DALMAWLRQWAASFGTVI"
     gene            complement(3678..4235)
                     /locus_tag="PLF56_02635"
     CDS             complement(3678..4235)
                     /locus_tag="PLF56_02635"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_004810534.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_function: GO:0016491 - oxidoreductase activity
                     [Evidence IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="TlpA disulfide reductase family protein"
                     /protein_id="HOQ52491.1"
                     /translation="MRRLVLTFATVVLLVLAGCTSGASADGGFVSGDGTITTVPVAQR
                     VAAPEISGTTLDGRQWSSTSVQGKVLVYNVWGSWCQPCIKEAPALAKASARTAASAQF
                     VGLNSRDIGKPQAQAFVREFGITYPNLYDPAGELVLQFAGKLPLSAIPSTVIVDTQGR
                     VAARVLGEASEATLVGLIDDVAAGR"
     gene            complement(4235..4888)
                     /locus_tag="PLF56_02640"
     CDS             complement(4235..4888)
                     /locus_tag="PLF56_02640"
                     /EC_number="3.1.3.-"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_014845563.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="histidine phosphatase family protein"
                     /protein_id="HOQ52492.1"
                     /translation="MQSVVHLVRHGKVHNPDGILYGRLPGYGLGEVGQAMAQRLGEYF
                     ADVPLTHLRCSPLQRAQETMAPIAANHPELEVVTDERVIEAANYFEGSNFGRYNEKLL
                     IPKNLWQLRNPLKPSWGEPYTSIVARVRLAIDDAAQAAGDDGQALIVMHELPIWISRL
                     AAEGRSLVHDPRKRECRLASVTSFTLIDGRVVAVDYAEPAADLVPPKASRVLFRPGS"
     gene            complement(4961..5371)
                     /locus_tag="PLF56_02645"
     CDS             complement(4961..5371)
                     /locus_tag="PLF56_02645"
                     /inference="COORDINATES: ab initio
                     prediction:GeneMarkS-2+"
                     /note="Derived by automated computational analysis using
                     gene prediction method: GeneMarkS-2+."
                     /codon_start=1
                     /transl_table=11
                     /product="hypothetical protein"
                     /protein_id="HOQ52493.1"
                     /translation="MHESLRAQLLTRLRDFGFSCREAADGAVILFDTTAGDGGYESQC
                     DVPDALLNEYLAARTPLSSTIPTEQRAQAIIDDVAAQVLETMQSEGGAPVQRFGLRQV
                     GHAVEWFSVRQDGPALNVAPDPEVSYWSAHSDGP"
     gene            complement(5412..5879)
                     /locus_tag="PLF56_02650"
     CDS             complement(5412..5879)
                     /locus_tag="PLF56_02650"
                     /inference="COORDINATES: ab initio
                     prediction:GeneMarkS-2+"
                     /note="Derived by automated computational analysis using
                     gene prediction method: GeneMarkS-2+."
                     /codon_start=1
                     /transl_table=11
                     /product="hypothetical protein"
                     /protein_id="HOQ52494.1"
                     /translation="MSRCVRLDVPLPADALFDYLSDPQRRPDWQASLRAVADVQGAGE
                     LGTTWTDCTAVGARPRLRVTGYERPRVWIERGTWQGVAAVLRLDLLPYGVDRTRLTAT
                     YAITGSGLFALPGAVLERLAGPAIAADLRRAVRLAAPTPGGSNLTWGSSRTQR"
     gene            complement(5888..6127)
                     /locus_tag="PLF56_02655"
     CDS             complement(5888..6127)
                     /locus_tag="PLF56_02655"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_011753984.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="glutaredoxin family protein"
                     /protein_id="HOQ52495.1"
                     /translation="MTPPRVLVIVRDGCHLCADALATAERVCAELGADFGTLDVDGDL
                     ALAEHTDHVPVTFVDGERLAIWFLDERQLRAALAG"
     gene            complement(6141..6242)
                     /locus_tag="PLF56_02660"
     CDS             complement(6141..6242)
                     /locus_tag="PLF56_02660"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_003402602.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="AURKAIP1/COX24 domain-containing protein"
                     /protein_id="HOQ52496.1"
                     /translation="MGSVIKKRRKRMAKKKHRKLLKKTRIQRRRAGK"
     gene            complement(6450..6614)
                     /locus_tag="PLF56_02665"
     CDS             complement(6450..6614)
                     /locus_tag="PLF56_02665"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_007078922.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_function: GO:0003677 - DNA binding [Evidence IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="helix-turn-helix domain-containing protein"
                     /protein_id="HOQ52497.1"
                     /translation="MKVTEVASMLRVSRMSVYRLIHAGELEAVRFGRNFRVPEQAVNN
                     YLHQSFYNAG"
     gene            7219..8826
                     /locus_tag="PLF56_02670"
     CDS             7219..8826
                     /locus_tag="PLF56_02670"
                     /inference="COORDINATES: ab initio
                     prediction:GeneMarkS-2+"
                     /note="Derived by automated computational analysis using
                     gene prediction method: GeneMarkS-2+."
                     /codon_start=1
                     /transl_table=11
                     /product="hypothetical protein"
                     /protein_id="HOQ52498.1"
                     /translation="MRPPLERASSLTALVGLALSISLLGSPTGAHAEQPAKPAESTTV
                     SHANVLSPAVKNLIRGTGIESEAKALETYWTPARMKAAKPVDGTLTVNRVGAAPKRTQ
                     ATQADGPAVSIAPLKVASKAIGTVKSGAKKPQTTYPGYPSSSPAARTNGKIFFSRDGS
                     NYVCSGSVVNSEGRSLVWTAGHCLGENGTWSYNVAFVPAYSNGSRPYGSWYAKDLATK
                     TAWLYDSDHANDVGAITLAPNAGVRIADQLGAQGLMWNQSANYWASAFGYPAVSPYTG
                     QVLVRADGNTADEGDGTIYMYSGLTGGSSGGPWLRNFDENWGYVNGHNDFIYTASPSW
                     MYSPYYGAQVASLYEFVRYNEVYALATATPTISGTAKVGNTLTAKPGNWKPSSVNLAF
                     QWKANGKAIAGAITPKLTLPASIKGATITVTVTGSKLGYTTASKTSKATKKVAAGTLS
                     GATPTISGSTKVGQLLTANPGAWKPSPVTLTYAWYRNGKAIKGATSSAYTLTKSDKGK
                     KITVKVTGKKAAYTTLTKTSKKTAKVK"
     gene            complement(8849..>9164)
                     /locus_tag="PLF56_02675"
     CDS             complement(8849..>9164)
                     /locus_tag="PLF56_02675"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_011753974.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=2
                     /transl_table=11
                     /product="pyrroline-5-carboxylate reductase dimerization
                     domain-containing protein"
                     /protein_id="HOQ52499.1"
                     /translation="VTAISGSGPAYLFFVVEAMIDAGVMLGLPRDIATLLVNQTMYGS
                     AKLLIESGSHPTVLREQVTSPGGTTAAALRQLEDHRVKAAFITAMEAARDRSRALAGP
                     AS"
BASE COUNT         1641 a         3226 c         2997 g         1300 t
ORIGIN      
        1 cccgcgtagc tgtgcaggcc cgagaccagc aggttgatgc cgacgaagtt gaaccagaag
       61 cagaccagcc cgacgatcgc gacgatcgcc gcccgcttgc cgcgccaacc cgcggtcgcc
      121 cgcgcgtgca ggtagccggc gtagaccacc caggtgacca acgaccaggt ctctttgggg
      181 tcccagcccc agaaccgtcc ccacgcgtac tcggcccaca ccgagccggc ggcgatggcg
      241 aaggtccaca gcggaaacgc gaaggcgtga aacttgtagg ccaactcgtc gaggcgctgc
      301 ggcgagggca gcttcgccag gtagccgcgc accttctcgc gacgcaccgc ccggatcttc
      361 atcagataca ggatcgacaa caacccgccc acgttgaacg cggcacccgc gacggcggcc
      421 gccacgatgt gaatcacgaa ccacaccgag tgcagcgccg gcaccaacgg cgcgacggcc
      481 acatagagca cggtgaccgc cagaccctgc ccgatcacca ccagcaggct caccggcagg
      541 cccaaccagc gcatgccgcg acatgcgagc accaggtaga tggtcaccac gaacagcaac
      601 gccgcggtga cgaactcgta catgttgccc cacggaaacc gctgcgccgc gatgccgcgg
      661 cagaccacac cggtcaggtg cagggcccag gcgatgatga tcagggcgag ccccatgcgg
      721 ccgaacaggt cgacctgcag gcgagccttg aggtcgcgcg cgtcggacga acccgcctcg
      781 gacgaggctc cctcggccga ccctcccccg gacgccaccc tctcggacgc tccgcgctgg
      841 ggacccgtca cgtcgtccgc ggcgccggat ggtacgtcgc cctccggccc ctcgaccccc
      901 atcacggcgc cgtcgctgcc gaccagcacc cccgcgggca gggccctttc cggctcgagc
      961 cggcgggcag ccgcccactc ggccgcgaac gccgacagtg caagcaggta gaccaccacc
     1021 gaggtgacca ttgccaggtt cgaccagtag gccgccatca gctcttccct tcgtcgtcgg
     1081 ctgtgccgcc gcccgtcgtg cccgccgggg gttccgccgc gtccggctcg gacgcaaccg
     1141 agcctgcgcc agcggcgggc gcgggcgcaa gccccagtcc gtccgagatc aggacgagtt
     1201 cgtcgtccaa tccgccgcga ccctcggtgc gatccaaccc cgccacctcg acccggtcgc
     1261 cgctcacctt gacccagacc cgtcggggcc gcacgaacag cgacaggcac aggcccgcga
     1321 cggccaccag caccgagccg aacaccagcc acaggccggg ggtgctgccg acctgcaggc
     1381 tcacccagcg cttccagtcg tgaaacgtga tcgagccgcc gccgggcagg tcgaacacct
     1441 caccgggggc cagcgtgaag cgcagcggtt tgccgtcctt ggtgtactgg gtcagcccgg
     1501 tggtgtcgag cgagtagacg ttctcgggca cccccgtggc ctctttgggg gccccgtgcc
     1561 aggcgttcag cagcagcagc gggttgagtg cgtcgggaaa gacggaggtt ccgccgttgg
     1621 cggagtcgac cgccgtgggc aggaacagcg cctcgaacgc caaccggtcc gggcgggcgt
     1681 agggcgcctt gaccaccccg acgctgagaa agttgccgtc ctgcggcagc aggatcaccg
     1741 gaccctcgaa ggcgaccttg ccgtcggcgc ccttgacggt cacgtcgacc gcgtacccgt
     1801 gccccaccag atggacggtg ctgtcgccca ccacgagcgg ctggttcacc tcgagcgtct
     1861 gctgtcggct gaccccgtcc atcgtggtgg tggtgttggc cttgaacacc cgggcggcgc
     1921 cgcgctgcac cggtccggtc tcgaacttgg cctcgaacga gtcgagtttc accgtgaacg
     1981 gcaccaactg cgaggcccgg aacgcgccgc ccgacgtgaa gtcgtcgaac tgggtcaggg
     2041 tgttgctgaa cgactggccc tcgaccagcg cgaccgtgcc cttgtaaccg aacagggcac
     2101 cccaggccac gccgcagagc agaaacacca gcgacacgtg aaagaccagg ttgcccagtt
     2161 cgcgcacgta gccgcgctcg gcgctgaccg agtcgggcag ccggcgcacc cggaagcgac
     2221 gcttgcgcaa cagcgtctcc gcttggtcga gcagtacggg ggtggcggcg ccggcgaagt
     2281 cgagatgggc cggcagtcgc cccaggttgc gcggagtcct cggcggctgc gcccgcagcc
     2341 ccctcaggta cacaccgatc cgcggaatga tgcagccgac gagcgacacg aacagcagca
     2401 ggtagatcgc cgcgaaccac ggcgacgcgt acacgtcgaa caagccgatc gcgtcgtaga
     2461 agccgccgag ttgcggattg ttggtgagga actcgcgcac ccgcaccggt gaggtgggac
     2521 gctgcggcaa cagcgagccc ggaatcgcgc cgagcgccag cgcgaacagc agcaccagcg
     2581 cggtgcgcat cgaggtgagc tgcgtccaca gccaacgcag ggtctcggac gcccgcagcg
     2641 tgccgccggg gcgcgtggca gccgtggtgc gccgggtcgg tgccttgccg ggtgagaccg
     2701 cacccgaggc gtccccgtga acctgccgac cggacgcgcc cgaggcgtcg tcgccaggcg
     2761 ccggacccgc acctgaggcg tcgcggccac cctcctgacc cgacgcgctc gacccgtcct
     2821 cgcggcccga ctcaccggac gcgcccgcgc tcgcctcgcc ggcggcctcc gcctcaggct
     2881 ggtcgaaggg gtcgatcagg tgcttgtcgg tgggcatcag atcaccgttc cgaacgaggc
     2941 ggcccactgc cgcagccaag ccatcagggc gtcccaccaa ccggtcaaca gcagcacgcc
     3001 gacggcgatc atcatcacgc cacccatcac ctgcagcgct cgatggtgcc gacggacgaa
     3061 gccgagcgcg ccaccgaacc ggtccagggc caggccggcc accagaaacg gcagtccgag
     3121 ccccagcgcg tagcagaagg ccagcagccc gccccgcagc gcggtcgcct cgttgagggc
     3181 cagggtgagt accacctgca gcgccggccc gatgcagggc gtccaaccca gggcgaacac
     3241 cagccccagc agcggcgcgg cggccaggcc gaccttgggt cgcaggttga gtcgcacggt
     3301 gcggctggcg aacggcaccc agcccgcgaa caccaggccg agtgcgatcg ccagcacccc
     3361 accgatcacg ttgatcgtgg tgcggtactg catcagggtg gtgcccagcg aaccgaacag
     3421 ggcgccaccc gacacgaaca ccacggcgaa gccgagcacg aacagcgagg tgccggccag
     3481 cacccgtcca cgactctcac cggcctgcaa ctgcgcggcg ctcagcccgc tggcgtagct
     3541 caggtagccg ggtagcagcg gcagcacgca gggcgagaag aacgacacca ggccggccag
     3601 cagggccacc ggaaccgcca gcagcatcga gccgcccacc gcctggcggg cccagtcccc
     3661 cacgtcgagc agaatcatca gcgtcctgcc gccacgtcgt cgatcaggcc gaccagggtg
     3721 gcctcggacg cctcgccgag cacccgtgcg gcgacgcgtc cctgggtgtc gacgatgaca
     3781 gtgctcggaa tggcgctcag cggcagcttg ccggcgaact gcagcaccaa ctcgcccgcc
     3841 gggtcgtaca ggttggggta ggtgatgccg aactcgcgca cgaacgcctg ggcctgcggc
     3901 ttgccgatgt cgcgcgagtt cagtccgacg aactgcgcgg acgcggcggt gcgcgcggag
     3961 gccttcgcca gggccggcgc ctctttgatg cagggctggc accacgagcc ccacacgttg
     4021 tagaccagca ccttgccctg gaccgacgtg ctgctccact gccggccgtc cagggtggtg
     4081 ccgctgatct cgggggctgc gacccgctgc gccaccggca ccgtcgtgat cgtgccgtcg
     4141 ccgctgacga aacctccgtc ggcggacgcg ccgctggtgc agccggccag caccagcagc
     4201 acaacagtcg cgaacgtgag gacgaggcgg cgcatcacga cccgggtcgg aacagcaccc
     4261 tggaggcttt cggcggcacc aggtcggcgg ccggttcggc gtagtcgacg gcgacgacgc
     4321 gtccgtcgat cagggtgaac gaggtgacgc tggccagccg gcattcgcgc ttgcgcgggt
     4381 cgtgcaccaa cgagcggccc tcggcggcga gccgcgagat ccagatcggc aactcgtgca
     4441 tcacgatcag cgcctgaccg tcgtcgccgg cagcctgcgc cgcatcgtcg atggccagcc
     4501 gcacccgcgc cacgatcgag gtgtacggct caccccagga cggcttcagc gggttgcgca
     4561 actgccacag gttcttgggg atcagcagct tctcgttgta cctgccgaag ttgctgcctt
     4621 cgaagtagtt ggccgcctcg atcacccgtt cgtcggtgac cacctcgagc tcgggatggt
     4681 tggcggcgat cggcgccatg gtctcttgcg cacgttgcag gggtgagcag cgcaggtggg
     4741 tcagcggcac gtcggcgaag tactcgccca gccgctgcgc catcgcctga ccgacctcgc
     4801 ccaggccata accgggcagc cgcccataaa ggatgccgtc ggggttgtgc accttgccgt
     4861 ggcgcaccag atgaactacc gattgcatgg gtgagggccg gcctttcgct gtgctcgggc
     4921 tcccaccata cgggacggac gcagcggcct cggcccttgc ctacgggcca tccgagtgcg
     4981 ccgaccagta ggaaacctct ggatcgggtg ccacgttcaa ggctggtcca tcctgtcgca
     5041 cgctgaacca ctcgaccgcg tgaccaacct ggcgaagtcc gaaacgctgc accggagcgc
     5101 caccctcgct ctgcatagtc tccagcacct gcgccgcgac gtcgtcgatg atcgcctggg
     5161 ctcgctgttc ggtcgggata gtggacgaga gtggggttcg cgcggccagg tattcattga
     5221 gcaaggcatc cggcacgtca cactgactct cgtacccacc gtccccagcc gtggtgtcga
     5281 agagaatgac tgcaccatct gccgcttctc gacagctgaa accgaagtca cgcagacgcg
     5341 tcaacagttg agctcgcagc gattcgtgca tggctcaatc atgctgtgta gcggggatgg
     5401 agtatcgctc ctcaccgctg cgttcgcgac gacccccagg tgaggttcga ccctccaggc
     5461 gtgggcgcag cgagccggac ggcccggcgc aggtcggcgg cgatggccgg cccggcaagt
     5521 cgttcgagaa cagccccggg tagggcgaac agccccgagc cggtgatcgc gtaggtggcg
     5581 gtgagacgcg tgcggtcgac accgtagggc agcagatcga gccgcagcac cgcggcgacg
     5641 ccctgccagg tgccccgctc gatccacacc cggggccgct cgtagcccgt cacccgcagc
     5701 cgcggacggg caccgacggc ggtgcaatcg gtccacgtcg tgccgagttc accggctccc
     5761 tgcacatcgg ccacagccct caggctggcc tgccagtcgg gacgccgctg cgggtcgctc
     5821 aagtagtcga acagagcatc ggcgggcagc ggaacgtcca agcggacaca gcgactcatg
     5881 cgagtgctca gccggccagc gcggcacgca actgccgctc gtccaggaac cagatggcca
     5941 ggcgttcacc gtcgacgaag gtgaccggta cgtggtcggt gtgctcggcc agggccagat
     6001 cgccgtccac atcaagggtg ccgaagtctg cgccgagctc ggcgcagacc cgctcggcgg
     6061 tcgccaacgc gtccgcgcac aggtggcagc cgtcgcgcac gatgaccagc acccggggcg
     6121 gggtcatcgg cgcgggccca ctacttgccg gcgcggcgac gctgaatgcg cgtcttcttc
     6181 agcagcttgc ggtgcttctt cttcgccatc cgcttgcgac gcttcttgat gaccgaaccc
     6241 acgctatgac ctctcgatgt cggcccaagg gcgggccggg taactcttcg ttgcggtgcg
     6301 gggcagaacc ccgaggactg accgggtgac ccagccaacc ccaagagctt accgagtgcg
     6361 tcggcacatc tgcgaattgc ggacgcaccc ggacgcgcga ccctgcgaat gcgtcggacg
     6421 atgcggcgat gcggaccgac cccgagcgct cagccggcgt tgtagaacga ctggtgcagg
     6481 tagttgttga cggcctgctc gggtacccgg aagttgcgac cgaaccgcac cgcctcgagt
     6541 tcgccggcgt ggatcagccg gtagaccgac atgcgcgaca ccctgagcat gctcgccacc
     6601 tcggtgacct tcatgaactg gatcgcaggc aggggcacgg gttggttcgc ggagtcgttc
     6661 actggagctc tctcactgtg gtcagcgtca cgccgacccc ccgatcggcg ctgagtgtcg
     6721 gccaccctac cccccggccg ggcccggccg cagcctcttg gccacaccgc gtgagcgtcc
     6781 accgtgccgg tgactggcgc cgtcgagacg ggtcgcgacc ccttctgacc aaggggttcg
     6841 acatcacgca accgcgatgt ccggcagccg atttgcaacc agtcacagga atttcgtctc
     6901 gctaaagtac gcaaatcctg agcttcttcc agagcttgaa caatgtggat tctcttgtca
     6961 ggactgctgc ccgttcgata actttcggat gggccgtcca actcgtccac cgtgcacgta
     7021 cgaaggattg cccatcccgg gggggaatca tgctcacatc gtgggcgggg tcgcgatcca
     7081 ttgtcgtgag tccagcgcgt acacgacgcc gatccgcaag cacgcgcgtc gagatatcag
     7141 ccctgactgg cgcaccacgc gcccgcagcg accactcctc gggcgcccac aaccacccac
     7201 gtgaggagat cttccgccat gcgaccaccc cttgaacgcg cgtcgtcgtt gacggcgctg
     7261 gtcgggttag cgctcagcat cagcctgctc ggttcaccaa ccggcgcgca tgcagaacag
     7321 cccgcaaaac cggccgagtc cacgacggtc agccacgcca acgtgctgtc ccccgccgtc
     7381 aagaacctga tccggggaac cggtatcgaa tcagaggcca aggcgctcga aacgtactgg
     7441 acgccggctc gaatgaaggc ggcgaagcca gttgacggca ccttgaccgt gaaccgggtg
     7501 ggcgccgcgc ccaagcgaac gcaggccacg caggccgacg ggccggccgt gagcattgcg
     7561 ccgctcaagg tagcgagcaa ggcgatcggt accgtgaagt ccggcgcaaa gaagccgcag
     7621 accacgtacc cgggctatcc gtcgagttcg cctgcggccc gcaccaacgg caaaatcttc
     7681 ttctcgcgcg atggttccaa ttacgtgtgc tcgggctcgg tggtcaacag tgagggccgc
     7741 agtctggtgt ggaccgcagg tcattgcctt ggtgagaacg gaacgtggtc gtacaacgtg
     7801 gcctttgttc cggcctattc caatggcagc cggccctacg gcagttggta tgccaaggac
     7861 ctggcgacca agactgcgtg gctgtatgac tccgaccacg ccaatgacgt cggggcgatc
     7921 acgttggcgc ccaacgccgg cgtccgcatc gccgaccaac tgggtgcaca ggggctgatg
     7981 tggaatcaga gcgcgaacta ttgggcaagt gcctttggtt acccggcggt ctcgccctac
     8041 accggccagg tgctggtacg tgcagacggg aacaccgccg acgagggcga cggcaccatc
     8101 tacatgtact cgggattgac cggtgggtca tccggcggtc cttggctgcg caacttcgat
     8161 gaaaactggg gttacgtcaa cggtcacaat gacttcatct acacggcgtc gccctcttgg
     8221 atgtacagcc cgtactacgg cgctcaggtc gccagcctgt acgagttcgt ccggtacaac
     8281 gaggtgtatg ccctcgccac tgccactcca acgatcagcg gtacggccaa ggtgggcaac
     8341 acgttgaccg caaagccggg taattggaag cccagttcgg tcaacctggc ctttcagtgg
     8401 aaagccaatg gcaaggccat cgcaggtgcc atcacgccga aactgaccct gcctgcctcc
     8461 atcaagggtg cgaccatcac cgtgaccgtg acgggctcga agctcggcta caccaccgcg
     8521 agcaagacct ccaaggccac caagaaggtg gctgcaggca cgctgagcgg cgccacgccc
     8581 acgatcagcg gcagcacaaa ggtcggccag ctgctcaccg cgaaccctgg tgcatggaag
     8641 ccgtctccgg tgacgttgac ctatgcgtgg taccgcaacg gcaaggcgat caagggcgcg
     8701 acctcgtccg cttacacgct gaccaagtcc gacaagggca agaagatcac cgtcaaggtg
     8761 accggaaaga aggcggccta caccaccctc accaagacgt ccaagaagac cgcgaaagtg
     8821 aagtagagca catggcgcgt tggtcggctc agctcgctgg gccggccaat gcccggctgc
     8881 ggtcgcgggc tgcctccatg gcggtgatga aggccgcctt gacccggtgg tcctccagtt
     8941 gccgcaacgc ggcggccgtg gtgccaccgg gcgaggtgac ctgctcacgc agcacggtgg
     9001 ggtgcgaccc cgactcgatc agtagcttcg ccgagccgta catggtctgg ttgaccagca
     9061 gggtggcgat gtcgcgcggc aggcccagca tcacgccggc gtcgatcatg gcctcgacca
     9121 cgaagaacag gtacgcgggg cccgatcccg agatggcggt gacg
//
LOCUS       DAOSUG010000047         9080 bp    DNA     linear   ENV 10-JUN-2024
DEFINITION  MAG TPA_asm: Micropruina sp. isolate AMR_MDS_2195
            NODE_4078_length_9080_cov_4.556343, whole genome shotgun sequence.
ACCESSION   DAOSUG010000047 DAOSUG010000000
VERSION     DAOSUG010000047.1
DBLINK      BioProject: PRJNA925477
            BioSample: SAMN32864262
            Sequence Read Archive: SRR4236673
KEYWORDS    WGS; ENV; Metagenome Assembled Genome; MAG; Third Party Data; TPA;
            TPA:assembly.
SOURCE      Micropruina sp. (wastewater metagenome)
  ORGANISM  Micropruina sp.
            Bacteria; Bacillati; Actinomycetota; Actinomycetes;
            Propionibacteriales; Nocardioidaceae; Micropruina.
REFERENCE   1  (bases 1 to 9080)
  AUTHORS   Abdulkadir,N., Saraiva,J.P., Zhang,J., Stolte,S., Gillor,O.,
            Harms,H. and Rocha,U.
  TITLE     Genome-centric analyses of 165 metagenomes show that mobile genetic
            elements are crucial for the transmission of antimicrobial
            resistance genes to pathogens in activated sludge and wastewater
  JOURNAL   Microbiol Spectr 12 (3), e0291823 (2024)
   PUBMED   38289113
REFERENCE   2  (bases 1 to 9080)
  AUTHORS   Abdulkadir,N.
  TITLE     Direct Submission
  JOURNAL   Submitted (24-MAR-2023) Environmental Microbiology, Helmholtz
            Center for Environmental Research -UFZ, Permoserstr 15, Leipzig
            04318, Germany
COMMENT     The annotation was added by the NCBI Prokaryotic Genome Annotation
            Pipeline (PGAP). Information about PGAP can be found here:
            https://www.ncbi.nlm.nih.gov/genome/annotation_prok/
            
            ##Genome-Assembly-Data-START##
            Assembly Method        :: SPAdes v. 3.11.1
            Genome Representation  :: Full
            Expected Final Version :: Yes
            Genome Coverage        :: Not Applicable
            Sequencing Technology  :: Illumina MiSeq
            ##Genome-Assembly-Data-END##
            
            ##Genome-Annotation-Data-START##
            Annotation Provider               :: NCBI
            Annotation Date                   :: 05/04/2023 04:57:10
            Annotation Pipeline               :: NCBI Prokaryotic Genome
                                                 Annotation Pipeline (PGAP)
            Annotation Method                 :: Best-placed reference protein
                                                 set; GeneMarkS-2+
            Annotation Software revision      :: 6.5
            Features Annotated                :: Gene; CDS; rRNA; tRNA; ncRNA
            Genes (total)                     :: 2,776
            CDSs (total)                      :: 2,750
            Genes (coding)                    :: 2,714
            CDSs (with protein)               :: 2,714
            Genes (RNA)                       :: 26
            tRNAs                             :: 24
            ncRNAs                            :: 2
            Pseudo Genes (total)              :: 36
            CDSs (without protein)            :: 36
            Pseudo Genes (ambiguous residues) :: 0 of 36
            Pseudo Genes (frameshifted)       :: 2 of 36
            Pseudo Genes (incomplete)         :: 32 of 36
            Pseudo Genes (internal stop)      :: 2 of 36
            ##Genome-Annotation-Data-END##
FEATURES             Location/Qualifiers
     source          1..9080
                     /organism="Micropruina sp."
                     /mol_type="genomic DNA"
                     /submitter_seqid="NODE_4078_length_9080_cov_4.556343"
                     /isolate="AMR_MDS_2195"
                     /isolation_source="Activated sludge of municipal
                     wastewater treatment plants"
                     /db_xref="taxon:2737536"
                     /environmental_sample
                     /geo_loc_name="Canada"
                     /lat_lon="49.25 N 123.1 W"
                     /collection_date="2015-06-16"
                     /metagenome_source="wastewater metagenome"
                     /note="metagenomic"
     gene            <1..1039
                     /locus_tag="PLF56_02680"
     CDS             <1..1039
                     /locus_tag="PLF56_02680"
                     /inference="COORDINATES: protein motif:HMM:NF012457.2"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_function: GO:0004553 - hydrolase activity, hydrolyzing
                     O-glycosyl compounds [Evidence IEA];
                     GO_process: GO:0005975 - carbohydrate metabolic process
                     [Evidence IEA]"
                     /codon_start=2
                     /transl_table=11
                     /product="family 1 glycosylhydrolase"
                     /protein_id="HOQ52500.1"
                     /translation="IEPEPGEFSVAELNHYRRVLETCHAHGLTPVVTFHHFTSPRWLL
                     AAGGWESPETPALFARYCDVAMHHLGDLIGMACTLNEPNLPYLLAELGLTGEPPEARR
                     QIPVWANAAAALGVEASTIAPFQFNATEVGYQVKLAAHRAGTAAIKAIRPEVPVGWTL
                     ANTDVQAGPGGEAYADRVRQNVNLRFLEAARGDDFVGIQNYGRHVFGPEGLLDPEPGA
                     PVNSQGEEIYPAGLANAVREAWQVAGVPVMITENGLATDDDTQRLAYLEAALGGLAEA
                     IADGVDVRGYIAWTAFDNFEWIFGYGPKFGLIAVDRTTQERTPKPSARWLGQVARSKG
                     AELDALTSAGV"
     gene            1041..2012
                     /locus_tag="PLF56_02685"
     CDS             1041..2012
                     /locus_tag="PLF56_02685"
                     /inference="COORDINATES: protein motif:HMM:NF013335.2"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="nucleoside hydrolase"
                     /protein_id="HOQ52501.1"
                     /translation="MASGSSGTRVRVLSDNDYAGDPDGLLQLAHQALSTSAELVAVIG
                     SHLPRNPSFDPRCDSADRAADEASTVLDLAGAEAVPVLAGSNVPLAARNTPIDTPAVR
                     YIIDAAMASDDRPLFTCFGGGLTELASAWLLEPRIAGRFTAIWIGGAEHPGLADPPPG
                     AGTAEFNASIDPIAAQVVLNDSDLDVWQVPRDAYRQVLVGMADLEDNLAGQGPLGDHL
                     LSRIESGEDFVRSHGLTPGEVMVLGDSPLVSLSTLQTTFEPSPASSRWVQRPAPLIAS
                     DGSYAGEDPRGRTVRVFTHIDTALIVRDLFAKVSRLARRASAGESGR"
     gene            2009..2803
                     /locus_tag="PLF56_02690"
                     /pseudo
     CDS             2009..2803
                     /locus_tag="PLF56_02690"
                     /inference="COORDINATES: protein motif:HMM:NF016497.2"
                     /note="incomplete; partial in the middle of a contig;
                     missing C-terminus; Derived by automated computational
                     analysis using gene prediction method: Protein Homology."
                     /pseudo
                     /codon_start=1
                     /transl_table=11
                     /product="glycoside hydrolase family 43 protein"
     gene            2977..3513
                     /locus_tag="PLF56_02695"
     CDS             2977..3513
                     /locus_tag="PLF56_02695"
                     /inference="COORDINATES: protein motif:HMM:NF036826.2"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="hypothetical protein"
                     /protein_id="HOQ52502.1"
                     /translation="MAPAGDPADFATPGPDVLRLTRVPDEGHRELLCTRVRHESWRAE
                     AAFDLTVGACRLVLRLDSRHWYEVQADGEVVRVTAGVGPFVHTVAEAPLPTRSVTLTL
                     AAQAPADAGAPGANRGPDEVLLGYRTSGSATVLARLDGRYLTTEVAGGFTGRMIGVGA
                     VDAAAHLQTFHYNPLEQD"
     gene            3515..5785
                     /locus_tag="PLF56_02700"
     CDS             3515..5785
                     /locus_tag="PLF56_02700"
                     /inference="COORDINATES: protein motif:HMM:NF036578.2"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="family 78 glycoside hydrolase catalytic domain"
                     /protein_id="HOQ52503.1"
                     /translation="MSHHTAGPLAEATFITADADRDAVLRFRTDVTLDQPADQVTSAV
                     VQATAHGVYELSIDGRPVTDSVLNPVWTAYEWRLQYQSFDVTELVRPASGPVRFEARV
                     GNGWYRGDLGFEGLAANYGDEIGVLAELVIDYADGARQVVGTSTEWTASTCEVTANSL
                     YNGQSIDARLRGTDGAPLTVRAGAFDASRLVAQVGPLVTRHEVLTPQRIWTSPSGRTL
                     VDFGQNLVGWVRLSVQGPAGTVVTVRHAEVLEHDELGTRPLRAAQATDRYTLSGGVDT
                     FEPTFTFHGFRYAEIEGWPGALDASALEAVVVHSAIAPTMTFECSDPLVNQLVSNSIW
                     GQKGNFLSVPTDCPQRDERLGWTGDIAVYAATAAAQFDVADFLHSWLLDLAAETVHHD
                     PPAVPVVIPDVLKYGHYAPDAPFSDVHSQAIWSDAAAWVPQALWQAYGDRDRLAAHYP
                     GMVAHLNSIEPLLSPTGLWDVGMQLADWLDPDAPPDAPWVAKADPGVVATASFYRSVA
                     FASEAATLLGYTSDAERWTVLADRLRTAFRAHYIADERITSDCATVYALAICFGLLDH
                     DELPWAARRLAEVVRARDHLVTTGFAGTPYVTWALSEHGYVEDAYKLLLQTGCPSWLY
                     PVTMGATTIWERWDSMLPDGTINPGDMTSFNHYALGAVADWIYQVVGGLRPAEPGWRS
                     VLIQPRPGPGIDWAKVSYTAAAGEVRSEWRSTDGTLELTVHVPAGLEATIVLPDGTRE
                     QRGEGRHEFTCAVAIG"
     gene            5907..6779
                     /locus_tag="PLF56_02705"
     CDS             5907..6779
                     /locus_tag="PLF56_02705"
                     /inference="COORDINATES: protein motif:HMM:NF014691.2"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="Fic family protein"
                     /protein_id="HOQ52504.1"
                     /translation="MGARYAQLASTHAEALHEITQAEVSEGVHQSNAIENSTLTLIDT
                     ERILAGGMLARRVNLREVFEARNLGAVTTSMSSLDTPPTTHDILKWHGQLMAGIRDDV
                     AGRFRRSNEWVRVGAHLGANPEFVPRLIEDALTHYAADHATYFMERIARFHCEFEIIH
                     PFLDGNGRIGRLVMNAQLQALGWPPVIVRAKNRHRDYYPALERYAITNRADAMTALLA
                     LLLQESLHKRIALLTSPHIIALQAWARSVGVRGNVAANQAKRQTIPAFRMRNRWMIAA
                     EHHPVQKPERGPRA"
     gene            complement(6853..7122)
                     /locus_tag="PLF56_02710"
     CDS             complement(6853..7122)
                     /locus_tag="PLF56_02710"
                     /inference="COORDINATES: ab initio
                     prediction:GeneMarkS-2+"
                     /note="Derived by automated computational analysis using
                     gene prediction method: GeneMarkS-2+."
                     /codon_start=1
                     /transl_table=11
                     /product="hypothetical protein"
                     /protein_id="HOQ52505.1"
                     /translation="MMKSRRVALSFVTALALAGAVFAVVAWRHGPTTGVVSPPDKPRD
                     YSLIGIADPGFDPKSLRDVKTEEDFKNLPGLSFDTAAPSPSPYRP"
     gene            7683..8564
                     /locus_tag="PLF56_02715"
     CDS             7683..8564
                     /locus_tag="PLF56_02715"
                     /inference="COORDINATES: ab initio
                     prediction:GeneMarkS-2+"
                     /note="Derived by automated computational analysis using
                     gene prediction method: GeneMarkS-2+."
                     /codon_start=1
                     /transl_table=11
                     /product="hypothetical protein"
                     /protein_id="HOQ52506.1"
                     /translation="MAGTQHRSVTGMDERTLGRIDLILFDAEYRDGKAAVQDSWRVAV
                     LASDPLTASAHERARAAAVGTANRRRDPTAARVPTSRRLHRAALGIALVSGVVAAALL
                     TVPRTSPLALQNVMLPAGVLAVVSVALLCWLEPRRANGSLWGSRVPAVIHLAFGGLWL
                     LGGAAVFVFRWGEVGLLAALPALAGLTLLGCAGLGALVLGEQALRADRSGRQTGIARV
                     TADLIDQRDAPEVFAALDRWWSGVGPEALQRSPARVRAVRREVLARLRAGMLITEPEE
                     RAAALAAKPEHWTERRR"
     gene            complement(8661..>9080)
                     /locus_tag="PLF56_02720"
     CDS             complement(8661..>9080)
                     /locus_tag="PLF56_02720"
                     /EC_number="3.4.24.-"
                     /inference="COORDINATES: protein motif:HMM:NF013594.2"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_function: GO:0004222 - metalloendopeptidase activity
                     [Evidence IEA];
                     GO_process: GO:0006508 - proteolysis [Evidence IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="M48 family metalloprotease"
                     /protein_id="HOQ52507.1"
                     /translation="DLVDVAYELNDFTAVKFVLAHELGHVKCRHVSLWRILTTSVVRF
                     VGLDSTITRAQEYTADRCASFYVPEGARTLVLLYAGKRVYRNVDIEAYMASVAAHKDG
                     LWLKLVNLFSSHPVGFRRMSALYQVQTRGWNIHGKML"
BASE COUNT         1401 a         3188 c         3056 g         1435 t
ORIGIN      
        1 catcgagccc gagcccggcg agttctcggt cgccgaactg aaccactacc ggcgggtgct
       61 cgaaacctgc cacgcccacg gcctcacccc ggtggtcacc tttcaccact tcacctcgcc
      121 gcgctggcta ctggccgccg gtggctggga gagccccgag acgccggccc ttttcgcccg
      181 ctactgcgac gtggcgatgc accacctggg cgacctgatc ggcatggcct gcacgctcaa
      241 cgagccgaac ctgccctacc tgctggccga gctgggcctg accggcgagc cgcccgaggc
      301 acgcaggcag atcccggtct gggccaatgc ggccgcggcg ctcggcgtcg aggcgtccac
      361 gatcgcgccg ttccagttca acgccaccga agtgggctac caggtgaagc tggccgctca
      421 tcgcgccggg accgccgcca tcaaggcgat ccgtcccgag gtgccggtcg gctggaccct
      481 ggccaacacc gacgtccagg ccggtcccgg cggcgaggcg tacgcggatc gcgtccgaca
      541 gaacgtgaac ctgcggtttc tcgaagccgc ccgcggcgac gacttcgtcg gcattcagaa
      601 ctacggacgc cacgtgttcg gccccgaggg cctgctcgac cccgaacccg gcgctccggt
      661 caacagtcag ggtgaagaga tctaccccgc agggctggcg aacgccgtcc gcgaggcctg
      721 gcaggtcgcc ggcgtgccgg tgatgatcac cgagaacggc ctggccaccg acgacgacac
      781 ccagcggctg gcctacctcg aggccgcact cggcggtctg gccgaggcga tcgccgacgg
      841 tgtggacgtg cgcggctaca tcgcctggac cgccttcgac aacttcgagt ggatcttcgg
      901 ctacggcccc aagttcggcc tgatcgcggt cgatcgcacc acccaggagc gcacccccaa
      961 gccgagtgcc cgctggctcg gccaggtggc ccgcagcaag ggcgccgaac tcgacgcact
     1021 cacctccgcg ggggtgtgag atggcgtccg gatcgtcggg cacccgggtt cgcgtcctca
     1081 gcgacaacga ctacgccggc gacccggacg gcctgctcca gctggcgcat caggctctgt
     1141 cgacctcggc cgaactggtg gccgtgatcg gttcacacct gccacgtaac cccagtttcg
     1201 acccgcggtg tgactccgct gatcgggcag cggacgaggc gagcactgtg ctcgacctgg
     1261 cgggcgccga ggccgtcccg gtgctggccg gctcgaatgt gccattggcg gcgcgcaaca
     1321 cgccgatcga caccccggcc gtgcgctaca taatcgacgc ggcgatggcg agcgacgatc
     1381 gtccgttgtt cacctgtttc ggcggcggac tcaccgaact ggccagcgcc tggttgctgg
     1441 agccacgcat cgcgggtcgg ttcaccgcca tctggatcgg tggcgccgaa caccccgggc
     1501 tggccgaccc tccgcccggt gccggtaccg ccgagttcaa cgcgtccatc gatccgatcg
     1561 ccgcccaggt ggtgctcaac gactccgacc tcgacgtctg gcaggtgccc cgggacgcct
     1621 accggcaggt gttggtcggc atggccgacc tggaggacaa cctggccggg cagggaccgc
     1681 tgggcgatca cctgctcagc cggatcgaga gcggcgagga cttcgtgcga tcgcatgggc
     1741 tgactcccgg cgaggtgatg gtgctgggcg attcgccgtt ggtgtcgctg agcaccctgc
     1801 agaccacctt cgaacccagc cccgcctcgt cccgatgggt ccagcgaccc gcaccactga
     1861 tcgcgtcgga cggtagttac gccggcgagg atccccgtgg acgaacggtg cgggtgttca
     1921 cgcacatcga caccgcactg atcgttcgcg acctgttcgc gaaggtgtca cggctcgccc
     1981 ggcgcgcatc ggctggggag agcggccgat gaccagcgtg aacgtagccg acgcatcggc
     2041 atcgaggctg ctctcgagcg tcccggctat tccgggcttc catcccgacc cgacgatctg
     2101 ccgggtcggc tcgacctact acctggccaa ctccagcttc gagtattttc ccggcgctcc
     2161 gctgtggcgc tccaccgacc tgtcgtcgtg gcagcaggtg ggcaacatcc tcacccgccg
     2221 ctcacagttc accgtgggcg acgggcgcga ctccggcggc gtgttcgggt ccacgctgcg
     2281 gcaccacgac ggcaggtact ggttcatcac caccaacatg agcgacttcg gccgcggcca
     2341 cacgctcgtg tcggccgaag acccggcgaa gtggagcgag cccctgtaca cgacgggcgc
     2401 gatcggcatc gatcccgacc tcagctgggg tccggacggg cagtgctacc tgacctggtg
     2461 cagcttcgcg cacggcggcg cgatcgcgca ggcgcggctc gacctcgcca ccggcgccct
     2521 gcaggaggaa cccagggtga tctggacggg cactgggctc gcctttcccg agggcccaca
     2581 cctgtaccgg cacggcgggt actggtacct gatgatcgcc gaaggcggca ctgagcgtgg
     2641 tcatgcggtg agcatcgccc gggcaccgca tccgaccgga cccttcgagt cgcacccggc
     2701 caatcccgtg ttcagccacc ggtcaacggc gcacccggtg cagaacgtgg gtcacgccga
     2761 cctggtcgag cgccccgacg gcacgtgggc ggcggtgtac ctcggctgcg gccccgtggc
     2821 agcacccccg gttactcgcc cctgggccgg gagaccttcg tcgcatccat cgactgggtg
     2881 gatgagtggc ccgcgttcgg gccgtccgac accatccctg ccgcggtcct tggcttcacc
     2941 gatcacttca ccggatcggc actcgacccg cgctgggtgg cgcccgccgg tgaccccgcc
     3001 gacttcgcca ccccagggcc ggacgttctg cgtctcaccc gggtgccgga cgagggccac
     3061 cgcgaactgc tgtgcacgcg ggtccgccac gagagctggc gagccgaggc cgcgttcgac
     3121 ctgaccgtcg gcgcgtgccg gctggtgctg cgactcgact ctcggcactg gtacgaagtg
     3181 caggccgatg gagaggtcgt ccgggtcacg gcgggggtag gccccttcgt ccacacagtc
     3241 gcagaggcac cacttcctac gcgctcggtc accctgacgc tcgcggccca ggcacccgcc
     3301 gacgcaggcg cgccgggcgc caatcgcggc ccggacgagg tgctgctcgg ctaccggacc
     3361 agcggatccg ccacggtgct cgcccggctc gacggccgtt acctcaccac cgaggtcgcc
     3421 ggcgggttca ccggccgcat gatcggcgtc ggcgccgttg atgccgcagc acacctgcaa
     3481 acttttcact acaaccctct tgaacaggac tgacatgagt caccacaccg ctggccccct
     3541 cgccgaggcg accttcatca ctgccgacgc cgaccgtgac gcggtgctcc gctttcgcac
     3601 cgacgtgacc ctcgatcagc cggccgacca ggtcacgtcc gcggtcgtgc aagccaccgc
     3661 gcatggcgtg tacgagctga gcatcgacgg ccgtccggtc accgattcgg tgctcaaccc
     3721 agtctggacg gcgtatgagt ggcgactgca gtaccagagc ttcgacgtca ccgagctggt
     3781 gcgcccggcg tccggcccgg tgcgattcga ggcccgcgtc ggcaacggct ggtaccgcgg
     3841 cgacctcggc ttcgaagggc tcgcggcgaa ctacggcgac gagatcggcg tgctggccga
     3901 gctggtgatc gactacgccg acggtgcccg ccaggtggtc ggcacctcga ccgagtggac
     3961 ggcgtccacc tgcgaggtga cggcgaactc gctctacaac ggccagtcga tcgatgcccg
     4021 gctgcgggga acggacggcg cgccgctgac cgtccgggcc ggtgcgttcg acgcctcccg
     4081 gctggtggcg caggtcggcc ccctggtcac ccggcacgag gtgctgacgc cgcagcggat
     4141 ctggacctcc ccgtccggac gcaccctggt cgacttcggt cagaacctgg tcggctgggt
     4201 acggctttcc gtgcagggtc cggcaggcac ggtggtcacc gtgcggcacg ccgaggtgct
     4261 cgaacacgac gagctgggca cccgcccgct gcgggcggcg caggccaccg accggtacac
     4321 cctgtcgggc ggcgtcgata cgttcgagcc gaccttcacc tttcacggct tccgttacgc
     4381 cgagatcgag ggctggccgg gcgctttgga cgcgtccgcg ctcgaagcgg tggtcgtcca
     4441 ttcagcgatc gcccccacga tgaccttcga atgttccgac ccgctggtca accagctggt
     4501 cagcaactcg atctgggggc agaagggcaa ctttctctcg gtgcccaccg attgcccgca
     4561 gcgcgacgaa cggctgggct ggacgggcga catcgccgtc tacgccgcca ccgccgccgc
     4621 ccagttcgac gtcgccgact ttctgcactc ctggttgctc gacctggccg ccgagaccgt
     4681 gcaccacgac ccgccggccg tgccggtggt cattcccgac gtgctcaagt acggccacta
     4741 cgcaccggac gcgccgttca gcgacgtgca ttcgcaggcg atctggagcg atgccgcagc
     4801 ctgggtgccg caggcgttgt ggcaggccta cggcgaccgc gaccggctcg ccgcgcacta
     4861 ccccggcatg gtcgcccacc tgaactcgat cgagccgctg ctgtccccga ccggcctgtg
     4921 ggacgtcggc atgcagctcg ccgactggct cgacccggac gccccgccgg atgcgccctg
     4981 ggtcgccaag gccgacccgg gcgtggtggc gacggcctcc ttctaccgat cggtcgcgtt
     5041 cgcctcagag gcggcgacgt tgctggggta tacgtccgac gccgagcgct ggacggtact
     5101 ggccgacagg ttgcgcaccg ccttccgtgc gcactacatc gcggacgagc gcatcacgtc
     5161 cgactgcgcc acggtgtacg cgctggcgat ctgcttcgga ctgctcgacc acgacgaact
     5221 tccgtgggcc gcccgccggc tcgccgaggt ggtgcgcgcc cgcgatcacc tggtgaccac
     5281 gggcttcgcc ggtacgccgt atgtgacctg ggcgttgtcc gagcacggct acgttgagga
     5341 cgcctacaaa ttgttgctgc agaccggctg cccctcgtgg ctgtatccgg tgacgatggg
     5401 cgccaccacg atctgggagc gctgggattc gatgctgccc gacggcacca tcaaccccgg
     5461 cgacatgacc agcttcaacc actacgccct gggcgccgtg gccgactgga tctatcaggt
     5521 ggtaggcggc ctgcgccccg ccgaaccggg ctggcgcagt gtgctgattc agcctcgtcc
     5581 aggccccggc atcgactggg cgaaggtcag ctacaccgct gcggccggcg aggtgcgcag
     5641 cgaatggcgc agcacggacg gcacgctcga gctgaccgtg cacgtgccgg cgggcctcga
     5701 ggcgaccatc gtgctgccgg acggcacgcg cgagcagcgc ggcgaagggc ggcacgagtt
     5761 cacgtgtgcg gtcgcgattg ggtgatcgca gcatcgcgtc acatggctgg ccgaaagcgt
     5821 gtgaaatatg atgactcagt ggtgtttttc acgctttgtt ggcggtcgcg gtgatcaatg
     5881 aggccacgcg gtcgcggatc gcgaggttgg gcgcgcgata cgcgcagctt gcgagcaccc
     5941 atgccgaggc gctgcatgag atcacccagg ctgaggtgtc cgagggggtg catcagtcga
     6001 acgcgatcga gaactcgacc cttaccctga tcgacaccga acgcatcctc gccggcggca
     6061 tgctggctcg ccgggtgaac ctgcgcgagg tgttcgaggc cagaaacctg ggggccgtca
     6121 caacgtccat gtcgagcctc gatactcccc cgaccacgca cgacattctg aagtggcacg
     6181 gccaactcat ggctggcatc cgagacgacg tggcgggccg cttccgccgc tcgaacgagt
     6241 gggtgcgggt gggcgctcat ctcggcgcca atcctgagtt cgtccctcgg ctgatcgagg
     6301 atgcgctgac ccactacgcg gcggaccacg cgacctactt catggaacgg atcgcacggt
     6361 ttcactgcga gttcgagatc attcacccgt tcctggacgg caacggacgc atcggtcggt
     6421 tggtgatgaa cgctcaactg caggcgctcg gctggccgcc cgtgatcgtg cgggcgaaga
     6481 accggcatcg cgactactac ccggcgctgg agcggtacgc catcacgaac agggccgacg
     6541 caatgaccgc actgctcgcc ttgctgctgc aggagtcgct gcacaagcgg atcgcgctgc
     6601 tgaccagccc ccacatcatt gcgctgcagg cctgggcgag atctgtgggc gtgcgcggaa
     6661 acgtcgccgc caatcaggcc aagaggcaga ccatccccgc tttccgaatg cgaaaccgct
     6721 ggatgatcgc cgccgagcac caccccgtcc agaagcctga gcggggccca agggcctagg
     6781 gcatgccgac agcgcgggct ggtcaggagt cgtggcttct gcgccccgcg tcgccgctgt
     6841 gggtcggtcg ggtcaggggc ggtagggaga cgggctgggt gctgccgtgt caaaactcag
     6901 acccggcagg ttcttgaagt cctcttccgt cttcacgtcg cgtagggact tcgggtcaaa
     6961 gccgggatcc gcaatcccga tcaggctgta gtcacggggc ttgtctgggg ggctcacgac
     7021 gccggtggtg ggcccatggc gccacgcaac caccgcgaat acagcgccgg cgagggccag
     7081 ggcggtaacg aacgaaaggg cgacccgtcg gctcttcatc atggtcctcg ggtggttggg
     7141 gggctacacc gtcaagacag gctgacaccg gcccgctctc tcgtcaagaa cctgcatggc
     7201 aacgcttgac tgttcctgag agtgccctcg atacgttcga ggcagccccg acgcccaaag
     7261 aacgaagaga cacgctgtga aggtgccgaa gaccggcgtt gccctgggtt tctggctcgc
     7321 tcttgccttc accggatcca ccgatggcgc cgtcgcgagc ggcaccagac aggtggctgg
     7381 caagccggcc cgcacgaagg catgccgggc gtcttccccg ctcgccggcg acgccagggc
     7441 gtccgaatgt agtcgcgcag cagacgcaga tcggtggatg ggacttgctc ggacccatca
     7501 acaacacagc gttgtgctcg gcggcctgat cttcgaccac gaagccgccg agtacggcgc
     7561 gccccagccc gatcgacacc gcagccttca ttgcaacgaa agctgccctg tcgaagtgaa
     7621 gtggtggggc ctactctggg acgccacagc cgtccgaaat cttcatcgca cgggagggca
     7681 gcatggccgg aacccagcat cgttcggtca ccggtatgga cgagcgcacg ctcggccgca
     7741 tcgacctcat cctctttgac gcggagtatc gcgacggcaa ggccgctgtg caggattcat
     7801 ggcgggtcgc ggtgctggcg tctgatcctc tgaccgcctc ggcacacgaa cgcgcccggg
     7861 cggccgcggt cggaacggcg aaccgacgcc gcgaccctac ggccgcgcgg gtacccacct
     7921 cgcgtcggct gcatcgcgcc gcgttgggga tcgccctggt gtccggcgtg gtcgcggcgg
     7981 cgctgctgac cgtgccacgg acaagcccgc tcgccctgca gaacgtgatg ctgcccgccg
     8041 gcgtgcttgc ggtcgtctcg gtggccctgt tgtgctggct cgaaccccgg cgggccaatg
     8101 gatcgctgtg gggatcccgc gtgcccgcgg tgatccacct cgccttcggt ggtctgtggc
     8161 tgctgggcgg cgcggccgtg ttcgtgttcc gctggggaga ggtgggcctg ctcgcggcgt
     8221 tgccggcact ggcgggactg acgctgctgg ggtgtgccgg gctgggggcg ctcgtcctgg
     8281 gcgagcaggc gttgcgcgcc gaccggtccg gccgtcagac cggcatcgcg cgggtcaccg
     8341 ccgacctgat cgaccagcga gacgcacccg aggtgttcgc cgctctcgac cgctggtggt
     8401 cgggtgtcgg tcccgaggcg ttgcagcgct ccccggcccg cgtgcgcgcc gtgcgacgcg
     8461 aagtgctggc gaggctgcgc gccggcatgc tgatcaccga acccgaggag cgcgccgccg
     8521 ccctggccgc gaagcccgag cactggacgg agcgtcggcg gtgagtcgtt ccagctgggt
     8581 tcggcattga cttacagccg tgtagttatc cccactgtgg atgcacctgt ggataaccca
     8641 cgcaccagcc gtcccaaccg tcacagcatc ttgccgtgga tgttccagcc ccgggtctgc
     8701 acttggtaca gcgcgctcat ccggcgaaag ccgaccgggt ggctcgagaa caggttgacg
     8761 agcttcagcc acaggccgtc tttgtgggcc gcgacactcg ccatgtacgc ctcgatgtcg
     8821 acattgcggt agacccgctt gcccgcgtac agcagcacca gtgtccgggc gccctcgggc
     8881 acatagaacg aggcgcagcg gtcggcggtg tactcctgtg cgcgggtgat ggtcgaatcc
     8941 aggccgacaa atcgcaccac gctggtggtg agaatgcgcc acaacgacac gtgccggcac
     9001 ttcacatgac ccaattcgtg agccagcacg aacttgaccg cggtgaagtc gttcagctcg
     9061 tacgccacgt cgaccagatc
//
LOCUS       DAOSUG010000048         9042 bp    DNA     linear   ENV 10-JUN-2024
DEFINITION  MAG TPA_asm: Micropruina sp. isolate AMR_MDS_2195
            NODE_4095_length_9042_cov_4.104373, whole genome shotgun sequence.
ACCESSION   DAOSUG010000048 DAOSUG010000000
VERSION     DAOSUG010000048.1
DBLINK      BioProject: PRJNA925477
            BioSample: SAMN32864262
            Sequence Read Archive: SRR4236673
KEYWORDS    WGS; ENV; Metagenome Assembled Genome; MAG; Third Party Data; TPA;
            TPA:assembly.
SOURCE      Micropruina sp. (wastewater metagenome)
  ORGANISM  Micropruina sp.
            Bacteria; Bacillati; Actinomycetota; Actinomycetes;
            Propionibacteriales; Nocardioidaceae; Micropruina.
REFERENCE   1  (bases 1 to 9042)
  AUTHORS   Abdulkadir,N., Saraiva,J.P., Zhang,J., Stolte,S., Gillor,O.,
            Harms,H. and Rocha,U.
  TITLE     Genome-centric analyses of 165 metagenomes show that mobile genetic
            elements are crucial for the transmission of antimicrobial
            resistance genes to pathogens in activated sludge and wastewater
  JOURNAL   Microbiol Spectr 12 (3), e0291823 (2024)
   PUBMED   38289113
REFERENCE   2  (bases 1 to 9042)
  AUTHORS   Abdulkadir,N.
  TITLE     Direct Submission
  JOURNAL   Submitted (24-MAR-2023) Environmental Microbiology, Helmholtz
            Center for Environmental Research -UFZ, Permoserstr 15, Leipzig
            04318, Germany
COMMENT     The annotation was added by the NCBI Prokaryotic Genome Annotation
            Pipeline (PGAP). Information about PGAP can be found here:
            https://www.ncbi.nlm.nih.gov/genome/annotation_prok/
            
            ##Genome-Assembly-Data-START##
            Assembly Method        :: SPAdes v. 3.11.1
            Genome Representation  :: Full
            Expected Final Version :: Yes
            Genome Coverage        :: Not Applicable
            Sequencing Technology  :: Illumina MiSeq
            ##Genome-Assembly-Data-END##
            
            ##Genome-Annotation-Data-START##
            Annotation Provider               :: NCBI
            Annotation Date                   :: 05/04/2023 04:57:10
            Annotation Pipeline               :: NCBI Prokaryotic Genome
                                                 Annotation Pipeline (PGAP)
            Annotation Method                 :: Best-placed reference protein
                                                 set; GeneMarkS-2+
            Annotation Software revision      :: 6.5
            Features Annotated                :: Gene; CDS; rRNA; tRNA; ncRNA
            Genes (total)                     :: 2,776
            CDSs (total)                      :: 2,750
            Genes (coding)                    :: 2,714
            CDSs (with protein)               :: 2,714
            Genes (RNA)                       :: 26
            tRNAs                             :: 24
            ncRNAs                            :: 2
            Pseudo Genes (total)              :: 36
            CDSs (without protein)            :: 36
            Pseudo Genes (ambiguous residues) :: 0 of 36
            Pseudo Genes (frameshifted)       :: 2 of 36
            Pseudo Genes (incomplete)         :: 32 of 36
            Pseudo Genes (internal stop)      :: 2 of 36
            ##Genome-Annotation-Data-END##
FEATURES             Location/Qualifiers
     source          1..9042
                     /organism="Micropruina sp."
                     /mol_type="genomic DNA"
                     /submitter_seqid="NODE_4095_length_9042_cov_4.104373"
                     /isolate="AMR_MDS_2195"
                     /isolation_source="Activated sludge of municipal
                     wastewater treatment plants"
                     /db_xref="taxon:2737536"
                     /environmental_sample
                     /geo_loc_name="Canada"
                     /lat_lon="49.25 N 123.1 W"
                     /collection_date="2015-06-16"
                     /metagenome_source="wastewater metagenome"
                     /note="metagenomic"
     gene            172..1176
                     /locus_tag="PLF56_02725"
     CDS             172..1176
                     /locus_tag="PLF56_02725"
                     /inference="COORDINATES: protein motif:HMM:NF013567.2"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_function: GO:0016491 - oxidoreductase activity
                     [Evidence IEA];
                     GO_function: GO:0016651 - oxidoreductase activity, acting
                     on NAD(P)H [Evidence IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="Gfo/Idh/MocA family oxidoreductase"
                     /protein_id="HOQ52508.1"
                     /translation="MEPAIRWGILGAGGIASVFTENLLAEGLHVQAVGARDGARAQAF
                     ADRFGIAIAHAGYEALVADPEVDAVYVATTHNFHAEHALLAIAAGKPVLVEKAFTVTA
                     AEARRVAEASRAAGVFAMEAMWTRFLPSTDFVRSRIADGSLGDVRALITDHSQKLNPD
                     PKGRLHNPDLAGGALLDLGVYNVSFALDLLGVPSEIVARGSLTATGVDAEVASILTHA
                     GGARSEAHTTMLSAGPSRATVIGTEGYLELDGAFYNWTTVRRYSGRQELVETFEPQRT
                     SRGMHFQALELQRCVREGLPESPRLTLDDSISVMTVLDDLRRQVGCVLPQDAVGTQTF
                     "
     gene            complement(1408..2163)
                     /locus_tag="PLF56_02730"
     CDS             complement(1408..2163)
                     /locus_tag="PLF56_02730"
                     /EC_number="3.4.-.-"
                     /inference="COORDINATES: protein motif:HMM:NF021592.2"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_function: GO:0070007 - glutamic-type endopeptidase
                     activity [Evidence IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="YhfC family glutamic-type intramembrane
                     protease"
                     /protein_id="HOQ52509.1"
                     /translation="MLLSAAQLLAIAIELTLMIGLPVALAIWWCRRTRVPWSAPLIAG
                     AFYLLNLLVNVPLTAVLYPRLGLSPVLLTALTALTYGVCEELASWLSFRVGSLRRHRD
                     GDGAIAAGIGHGGLESMLFGLSYAAGAIAALIAPDALPETTRAALVGADPWIFIGTGL
                     DRLPALAGHLVFALLIVLAYRRGVRYLWLAIVAHAALDFTMFMVRDYAPTAVFIVVWA
                     LVGVLSLLLARRLYGGLPSGQPLSDKRGTAADG"
     gene            complement(2364..2702)
                     /locus_tag="PLF56_02735"
     CDS             complement(2364..2702)
                     /locus_tag="PLF56_02735"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_014847440.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="transcriptional regulator"
                     /protein_id="HOQ52510.1"
                     /translation="MSIKGGAGHKDAIDENLHSPVRFAIMASLVNLDKAEFAFVRDAL
                     EVSDSTLSKQATALESLGYLKVIKGHVGKRPRTWFALTPHGRTAFAGHLQALRSLAEA
                     AARFSPAAPA"
     gene            complement(2699..3115)
                     /locus_tag="PLF56_02740"
     CDS             complement(2699..3115)
                     /locus_tag="PLF56_02740"
                     /inference="COORDINATES: ab initio
                     prediction:GeneMarkS-2+"
                     /note="Derived by automated computational analysis using
                     gene prediction method: GeneMarkS-2+."
                     /codon_start=1
                     /transl_table=11
                     /product="hypothetical protein"
                     /protein_id="HOQ52511.1"
                     /translation="MDDPDNALTQADRLAAHTRTSARWLARYYVIFGVASLLMPLGFG
                     LLHEVAWTITLLMFWLALIVGISIYAGRQPTMVQGGGRIHAAVMVAWTVIWVTTVTVG
                     SSQNLPWPWWLAGGVGMLAAALLGARAVQSRTRGPA"
     gene            complement(3159..3542)
                     /locus_tag="PLF56_02745"
     CDS             complement(3159..3542)
                     /locus_tag="PLF56_02745"
                     /inference="COORDINATES: protein motif:HMM:NF014687.2"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="DUF202 domain-containing protein"
                     /protein_id="HOQ52512.1"
                     /translation="MPRRLNSPNPDDAPRRPDQVWSGGLQPERTSLAWQRMNLAGLAA
                     SLVGARLVMQAHPVLGYVLATLSAAAAAVITVEHTRRSRRLHYSLVGGGPLPDGRIHL
                     LAAGLLLLVAVGGLVFVLSAAPFSW"
     gene            complement(3899..4276)
                     /locus_tag="PLF56_02750"
     CDS             complement(3899..4276)
                     /locus_tag="PLF56_02750"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_017936005.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="DUF202 domain-containing protein"
                     /protein_id="HOQ52513.1"
                     /translation="MPTTPPANRWPAWVYGVGDDPDPRFSLANERTFLSWIRTGLAFI
                     TAGLGIAALARFAVASSSQFSIAAIVLLGCGVLCGVTGFTRWARNERAMRLRNPLVAP
                     SSLPVLSVILLLIAGLAGFMLIG"
     gene            4344..6245
                     /locus_tag="PLF56_02755"
     CDS             4344..6245
                     /locus_tag="PLF56_02755"
                     /inference="COORDINATES: ab initio
                     prediction:GeneMarkS-2+"
                     /note="Derived by automated computational analysis using
                     gene prediction method: GeneMarkS-2+."
                     /codon_start=1
                     /transl_table=11
                     /product="hypothetical protein"
                     /protein_id="HOQ52514.1"
                     /translation="MIDMTDNGSVPGQNGPGDQGGRPPQVFGNQPGGWQSPPPAQGLP
                     PTAATPSVQPGWGQQSSGQGPSAGTPPVQPGWGQQSSGQGPSAGTPPVQPGWGQQPGQ
                     QAWPQGAAQYGGAQPGGAQYGGAQPGGAQPGGAQYGASPQGGAQYGGAQPGGAQHDAT
                     PQGATQYGATQPGGPQYGGAQPGGQYGAQPGGQYGAQPGGAQYGPTQPDGAQYGTQPG
                     GQYGVTPPGNTSYGQPYPPAGTGAPYGPPSSTDQGRQPGQQPAAGWAPSVGAQHPQAP
                     WGAQPGGPGQQAWGYPPSGMGGVPGPNQPRRSNRAVLGVIGGVVGVALIGGTVLAVQR
                     NNTSNPTPQPSATASNGQSVPPAAVQKPSDLVSSYLKALGTGDAQGALSMAAVTPTDT
                     TMLTDEMLAKSTAGKLTDISVPEVSDENATSVRASYTLNGKPVTATFGITRIAGLLRL
                     EQVAADVDVSSVSNVAVTVGGVRPKSDVVTVFPGSYAVAPVNKYYALATKSLAVNDLE
                     ALTPATRNATISSSGRSAIIKAVNSTYKACLKRHSTKPGCGFGVSTRKGVTLRTNTIK
                     WTTRSGAKWSKIKPRLLAEGVAEASAPATVHFYARDAKVSGRYWYKDIKLRGVSLTIG
                     SNKVSVAFY"
     gene            complement(6601..8463)
                     /locus_tag="PLF56_02760"
     CDS             complement(6601..8463)
                     /locus_tag="PLF56_02760"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_020390009.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_component: GO:0016020 - membrane [Evidence IEA];
                     GO_process: GO:0006812 - monoatomic cation transport
                     [Evidence IEA];
                     GO_process: GO:0055085 - transmembrane transport [Evidence
                     IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="cation:proton antiporter"
                     /protein_id="HOQ52515.1"
                     /translation="MEILLLVVGCVSTVLLVTALGRRLPVPAPLLLLVVGAVASYEPW
                     FPLPELTAEMALFGFLPPLLYAAAVNTSLVDFKDNLPAIGWLSIGFVLFCALGVAAVI
                     SGMLPVPFAAAFAIGAIVAPPDAVAAVAVARRSGLPRRVVTILEGESLLNDATALVAL
                     RTAVTALAATVTFWDVGTDLLLAVVVGPLAGWLVAKVSIWLFRVLTEPVMSTTLTFLI
                     PFAAYALAESLHASGVLAVVVAGLIVGHVTPTMRSAPARVATWMNWSTIQYVLEHTVF
                     LLIGLYTHEILDAASTYPVSNLTIALVSGAVLLAVIGLRIVYVMAVRPFLSRRHRPLS
                     WREAAIISWAGMRGVVTLAAALTLPVDTPLRPVLLLIALVTVAGTLLIHGLTLPLLAR
                     RLGVRGPDPREDALQEAMVTQRSITAGMTALKAVAGPDDAELVARLKTIAKRRIDLQW
                     ERLGRAKEGSESSAMAQRRLRLAAMAGERAKLLEIRDKGFADHEVLSTLLTELDVEEA
                     TLVNREDREREVREAPPRVEGEAPCVHLEEAPAEVDFEAHGECEDCVRLGQEPVELRM
                     CLTCGHIACCDSSPGKHATLHFRRTGHPVMRSIEPGEHWRWCYIDQLHDEGPDD"
     gene            8648..>9042
                     /locus_tag="PLF56_02765"
     CDS             8648..>9042
                     /locus_tag="PLF56_02765"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_011756633.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="proline--tRNA ligase"
                     /protein_id="HOQ52516.1"
                     /translation="MITRLSTLFVRTLREDPADAEVASHRWLVRAGYIRRVAPGIYSW
                     LPLGLKVLAKVERIVREEMDALGGQEVRFPALLPREPYETTHRWTEYGPNLFRLKDRK
                     GGDYLLGPTHEEMFTLLVKDLYSSYKDLPL"
BASE COUNT         1511 a         3108 c         3203 g         1220 t
ORIGIN      
        1 accctcgctc gacaaaagac ggcgggcgag ccccgcagga tgcaggacga cctgcccgga
       61 agccgagcct gccccagtac tgtgcagagg tgacgtcctg cttcgccggc cgtcgccgcc
      121 cgctcgtcgc ggcggcatgc agccatcctg aatctgattg gagatcacga catggagcca
      181 gccatccgct ggggcattct cggggccggg ggcatcgcga gcgtcttcac cgagaacctg
      241 ctggccgagg gactgcacgt gcaggccgtc ggggcccggg acggcgctcg cgcccaagcc
      301 ttcgccgaca ggttcggcat cgccatcgcg catgcgggct acgaggccct ggtcgccgac
      361 cccgaggtcg atgcggtcta cgtggccacc acgcacaact ttcacgccga gcatgcgctg
      421 ctggccatcg cggccggcaa gccggtgctg gttgagaagg cgttcaccgt gacggccgcc
      481 gaggcgcgtc gggtcgccga ggcctcgcgg gccgccggcg tcttcgccat ggaggccatg
      541 tggacccgct tcctgccctc cacggacttc gtccgcagcc gcatcgccga cggcagcctc
      601 ggcgacgtgc gcgccctgat caccgaccac agccagaagc tcaaccccga ccccaagggc
      661 cggctgcaca accctgacct ggctggtggc gcgctgctcg acctcggcgt ctacaacgtg
      721 tccttcgcgc tcgacctgct gggggtgccg tccgagatcg tggcgcgggg cagcctgacc
      781 gccaccggtg tggacgccga ggtcgcctcg atcctgaccc atgccggggg cgcgcggtcc
      841 gaggcgcaca ccacgatgct cagcgcggga ccgtcccgtg cgacggtgat cggcaccgag
      901 ggctacctcg aactcgacgg cgccttctac aactggacga cggtgcgccg ctacagcggt
      961 cgccaagagc tggtcgagac cttcgaaccg caacgcacct cgcgcggcat gcactttcag
     1021 gccctcgaac tgcagcgctg tgtgcgcgag ggactgcccg agagcccgcg gctcacgctc
     1081 gacgactcga tctcggtgat gacggtgctg gacgatctgc gccgccaggt cggctgtgtg
     1141 ctgccccagg acgcggtcgg cacccagact ttctgaaatc cggacaccag cgccaccctc
     1201 accccgcgac acgccgtgcc ggctcgcgac cctggcgggt gatctcagaa tcgcgctgtc
     1261 ggctcgccag ggatgaccaa tcagctcgcc gcggtaggga gtgggctcgg ccggcggctg
     1321 tttcgggatg agtggagggc tcgatgcgca acgagcgcga tcagccgacg gctgttttgc
     1381 catgagtggg gggctcgacg aaggcgatca gccgtcggcc gcggtgcccc tcttgtcgct
     1441 gagcggctga ccggacggca ggccgccgta caggcgacgg gcgagcagca ggctcagcac
     1501 gccgaccaag gcccacacca cgatgaacac ggccgtcggt gcgtagtcgc gcaccatgaa
     1561 catggtgaag tccaaggcgg cgtgcgcgac gatggcgagc cacaagtagc gcactccacg
     1621 gcggtaggcc agcacgatca gcagcgcgaa cacgaggtga cccgccaggg ccggcagccg
     1681 gtcgagtccc gtgccgatga agatccacgg gtcggcgccg accagtgcgg ctcgggtggt
     1741 ctcgggcagg gcgtccgggg cgatcagggc ggcgatagct ccggccgcat aggacagccc
     1801 gaacagcatc gattccaagc cgccgtgacc gatcccggcg gcgatcgcgc cgtcaccgtc
     1861 gcgatgccga cgcagcgagc cgacccggaa cgacagccaa ctggccagct cttcacacac
     1921 gccgtaggtg agcgcggtca gcgcggtcag cagaaccggc gacaacccca ggcgagggta
     1981 gagcaccgcg gtcaacggca cgttcacaag cagattcagc aggtagaacg caccggcgat
     2041 cagcggcgcg gaccagggca cccgggtgcg ccggcaccac cagatggcca gcgcgacggg
     2101 caacccgatc atcaaggtca gctcaatggc gatggccagc aactgggcgg cagaaagcag
     2161 catgacgact cctatgggtc gggcgcgggc acgatggtgc ccgcaacggg acgggtgagg
     2221 ctgatggtga ctggagacgg ccacggacga gggcgggtgg cggtcgccgg tggagtgcgg
     2281 cggcggccga ggcgcgtggt gacgttctcc gctcgagtgc ggcgagcatg gatcaggagg
     2341 gtcgcggtgg gcgcgacgga gggtcaagca ggtgctgcgg gactgaaccg agccgctgct
     2401 tcggcaaggg agcggagggc ctgaagatgg ccggcgaagg ccgtgcgtcc gtgcggggtc
     2461 agggcgaacc aggtgcgcgg acgcttgccg acgtgaccct tgatcacctt cagatacccg
     2521 agtgactcca gggctgtggc ctgcttcgag agcgtggagt ccgagacctc gagggcgtcg
     2581 cgcacaaagg cgaactcggc cttatcgaga ttcaccagac tggccatgat cgcaaaccgg
     2641 acgggcgagt gcaggttctc atcgatcgca tccttgtggc cggcaccccc tttgatgctc
     2701 atgccggccc cctggtgcgg ctctgcacgg cccgggcgcc gagcagagcc gcggccagca
     2761 tgccgacgcc accggccaac caccacggcc agggcaggtt ctggctgctg ccaaccgtca
     2821 ctgtggtcac ccagatcacc gtccaggcca ccatcactgc tgcgtgaatc cggccgccgc
     2881 cctgcaccat cgttggttgc cggccggcat agatcgagat acccactatc aaggcgagcc
     2941 agaacatcag cagcgtgatg gtccaggcga cttcgtgcag caggccgaag ccgagcggca
     3001 tgagcagcga ggcgacgccg aagatcacgt agtagcgggc aagccagcgg gcggacgtgc
     3061 gggtgtgcgc ggccagccgg tcggcctgcg tgagagcgtt gtccggatca tccatgctgc
     3121 cagtctagca agtactttca ctatcggaaa gtagttttct accaggagaa cggtgctgcg
     3181 ctgagcacga acaccagacc gccgacggcc accagaagca gcaggccggc cgcgagcaga
     3241 tggatccgtc catcgggcag cggaccgccg ccgaccagcg agtaatgcag ccgtcgtgat
     3301 cgtcgggtgt gttccacggt gatcacggcg gccgcagctg cactgagggt ggcgagcacg
     3361 tagccgagca ccggatgagc ctgcatcacc aggcgtgctc cgaccaggct ggccgccagc
     3421 ccggccaggt tcattcgctg ccacgccagg gatgtgcgct cgggttgcag accgccgctc
     3481 cacacctgat cgggccgacg gggcgcatcg tcggggttgg gactgttaag ccgacggggc
     3541 acatcgtcgg ggttgggacg ggtggacgca cggggtgggt cgttcgggtc gggacggtcg
     3601 ggccgacggg gtgcgtcgtt ccgatcgaga gagccagggc aagggaaagg gttgtccgga
     3661 tcgggactgc ccggacggtc ggcccggtgg tggggtgagt gggtggcgtc cgggagtcca
     3721 ggcgggtggg gttcgtcgtg cggcccagga ctgtcgggcc ggcggggcgg gttgctcgga
     3781 tcgggacggt cggcccggtg gggtgagtgg gtggcgtccg ggggtccagg cgggtcgggt
     3841 tcgtcgtgcg gcccaggact gtccggccgg ccgggcggat cgtatggatc cggcactttc
     3901 agccgatcaa catgaagccg gccaggccgg cgatcagcag caggatcacc gacagcaccg
     3961 gcagcgacga cggggcgacc agggggttgc gcagccgcat cgcccgctcg ttgcgcgccc
     4021 agcgcgtgaa cccggtgacg ccgcagagca cgccgcaacc cagcagcacg atggcggcga
     4081 tgctgaactg gctgctcgat gccacggcga agcgagccag ggcggcgatg ccgagcccgg
     4141 cggtgatgaa ggccaagccg gtgcggatcc aggacaggaa cgtgcgttcg ttggccaggc
     4201 tgaaccgcgg gtcggggtcg tcgccgacac cgtagaccca cgccggccag cggttggcgg
     4261 gcggcgtcgt cggcatgctg ccgaggatac gtccggcggt cgtgccgtgg gacctgccgg
     4321 ccgtagactg cccgcccagg gggatgatcg acatgactga caacggatcg gtgcccggac
     4381 agaacggccc gggcgatcag ggcggccggc cgccccaggt cttcggcaac cagccaggtg
     4441 ggtggcaatc gccgccaccg gcgcagggtc tgcccccgac cgcagctaca ccctcggtgc
     4501 agccgggttg gggtcagcag tcgtcggggc aaggtccgtc ggcgggtacg ccgccggtac
     4561 agccgggttg gggtcagcag tcgtcggggc aaggtccgtc ggcgggtacg ccgccggtac
     4621 agccgggttg gggtcagcag cccggccagc aggcctggcc tcagggtgcc gcccagtacg
     4681 gcggtgctca gcccggcggc gcccagtacg gcggtgctca gcccggcggt gctcagcccg
     4741 gcggcgccca gtacggcgcg tcgcctcagg gtggggccca gtacggcggt gctcagcccg
     4801 gcggcgctca gcacgacgcg acgcctcagg gcgcgaccca gtacggtgcg acgcagcccg
     4861 gcggccccca gtacggcggt gcgcaaccgg gcgggcagta cggggcgcag ccgggcgggc
     4921 agtacggtgc gcagcccggc ggcgcccaat acggcccgac gcagccggac ggggctcagt
     4981 acggcaccca gccaggcggg cagtacggtg tgacgccgcc gggcaacacc tcctacggcc
     5041 agccataccc gccggccggg accggcgcgc cgtacggtcc gccgtcgtcc accgaccagg
     5101 gacgacagcc gggccagcag cccgcggccg gctgggcgcc ctcggtgggt gctcagcacc
     5161 cgcaggcccc gtggggagcc cagccgggcg ggcccggcca gcaagcgtgg ggctacccgc
     5221 cgtccggaat gggcggtgtg ccgggcccga atcagccgcg acggtccaac cgggccgtgc
     5281 tcggcgtgat cggcggcgtc gtcggcgtgg cgctgatcgg tggcacggtg ctggccgtcc
     5341 agcgcaacaa caccagcaat ccgaccccgc agcccagcgc gacggcctcg aacggccaga
     5401 gcgtgccgcc ggcggcggtg cagaagccgt cagacctggt cagctcgtac ctgaaggcgc
     5461 tgggcactgg cgacgcacaa ggcgcgctga gcatggccgc agtgacgccc accgacacca
     5521 cgatgctgac cgacgagatg ctggcgaagt ccaccgccgg caagctgacc gacatttcgg
     5581 ttccagaggt gagcgacgag aacgccacca gcgtgcgtgc cagctacacg ctgaacggca
     5641 agccggtgac cgccacgttc ggcatcacca gaatcgccgg gctgttgcgc ctcgaacagg
     5701 tggcggcaga cgtcgacgtc tcgtcggtgt cgaacgttgc ggtgacggtg ggcggagtgc
     5761 gtcccaagtc ggacgtcgtc accgtctttc ccggcagcta tgccgtggcg ccggtcaaca
     5821 agtactacgc cctcgccacc aagtcactgg cggtgaacga cctggaggca ctgactcccg
     5881 ccacgcgcaa tgcgacgatc agctcgtcgg gacgctcggc catcatcaag gcggtcaact
     5941 ccacgtacaa ggcctgcctg aagcggcatt cgaccaagcc cgggtgcgga ttcggggtga
     6001 gtacgcgcaa aggtgtcacg ctgcgcacca acacgatcaa gtggacgacc cgcagcgggg
     6061 ccaagtggag caagataaag cccaggttgc tcgccgaggg cgtggccgag gccagcgccc
     6121 cggccaccgt gcacttctat gcccgcgacg ccaaggtgtc gggccgctac tggtacaagg
     6181 acatcaagct gcgcggggtg agcctgacga tcggctcgaa caaggtctcg gtcgcgttct
     6241 actgaccctg ccacgggcct gacacgccgg gtcctctcgc cgaggatgac aacgcagtga
     6301 gcaggcgtac tgggtgcgcc gcagcgcggc cagggtgagg agacacgccc taggtggttg
     6361 gtgtttttcg gccgttcaac accaaccacc tagatccgcc tgctgctgcg cgccggaccc
     6421 ggcgagcttt gggggatccg tgatgcgggt cgagacccgg ccaccgttgg tcgagcctgt
     6481 cgagacccat ccgctcggac cccttcatgt ctcgtcgcga ggcgcgggcg cacccgctca
     6541 gtcgtccggg ccctcgtcgt gcaattggtc gatgtagcac caccgccagt gttcgcccgc
     6601 tcagtcgtcc gggccctcgt cgtgcaactg atcgatgtag caccaccgcc agtgctcacc
     6661 cggctcgatc gagcgcatca ccgggtggcc ggtgcgccga aagtgcaggg tggcgtgctt
     6721 gcccggcgac gagtcgcagc aggcgatgtg gccacaggtc aggcacatgc gcagctcgac
     6781 gggttcctgg ccgagtcgca cgcagtcctc gcactcgccg tgcgcctcga agtcgacctc
     6841 ggcgggcgcc tcctccagat ggacgcaggg cgcctcaccc tcgacccggg gtggcgcctc
     6901 gcgcacctcg cgctcgcgat cttcgcggtt gacgagggtg gcctcctcga cgtccagctc
     6961 ggtgagcagc gtgctgagca cctcgtggtc cgcgaacccc ttgtctctga tctcgagcag
     7021 tttggcccgc tcgcccgcca tcgcggccag ccgcagtcgc cgctgggcca tagcgctgct
     7081 ctcggaacct tccttcgccc gtccgagtcg ttcccattgc aggtcgatcc gccgtttggc
     7141 gatcgtcttc aggcgtgcga ccagttcggc gtcgtccgga ccggcgacgg ccttcaatgc
     7201 cgtcatgccg gccgtgatcg agcgctgggt gaccatcgcc tcctgcagag cgtcctcacg
     7261 cggatcgggg ccccgcaccc ctagccggcg cgccagcagc ggcagggtca ggccgtggat
     7321 cagcagagtg ccggccaccg tcaccagtgc gatcagcagc aggaccggac gcaggggggt
     7381 gtcgaccggc agagtgagcg cggccgccag cgtcaccacc ccacgcatgc cggcccatga
     7441 gatgatcgcc gcttcgcgcc acgacaacgg acgatgccga cggctcagga acggccgcac
     7501 ggccatcacg tagacgatgc gcaacccgat cacggccagc agcacggcgc ccgacaccag
     7561 cgcgatggtg aggttcgaca ccggataggt cgaggccgca tcgagaatct catgggtgta
     7621 cagcccgatc agcagaaaca ccgtgtgctc gagcacgtac tgaatcgtcg accagttcat
     7681 ccaggtggcg acccgcgccg gcgccgagcg catggtcggc gtgacgtgcc cgacgatgag
     7741 gccggccacc accaccgcca gcacacccga ggcatgcaac gactcggcca gcgcatacgc
     7801 cgcgaacgga atcaggaacg tcagcgtggt gctcatcacc ggctcggtga gcaccctgaa
     7861 cagccagatc gacaccttgg cgacgagcca acccgccagc ggccccacca ccacggccag
     7921 cagcaggtcg gtgccgacgt cccagaacgt cacggtcgcg gccagggcgg tcaccgcggt
     7981 gcgcagggcg accagggcgg tcgcgtcgtt gagcagcgac tcgccctcga ggatggtcac
     8041 cacccggcgg gggagtcctg agcgtcgggc caccgcgact gcggcgaccg cgtccggtgg
     8101 ggcgacgatc gcgccgatcg cgaaggccgc cgcgaacggc accggcagca tgccgctgat
     8161 caccgcggcg acgcccaacg cgcagaacag cacgaagccg atcgacagcc agccgatcgc
     8221 cggcaggttg tccttgaagt cgaccagcga ggtgttcaca gcggcggcgt acagcagggg
     8281 cggcaagaat ccgaacagcg ccatctcggc ggtcaactcg ggcaacggaa accatggttc
     8341 gtacgaggcg acggccccca ccacgagcag cagcagcggt gccgggacgg gcagtcgccg
     8401 gcccagcgcg gtgaccagca gcacggtgct gacgcagccg accaccagca gcagtatttc
     8461 catggcccct cctcactgga cgcgcgccca tcctgcccgc cacggaccgg cgccgcacgc
     8521 acgtccagcg tgggtggccg caacccggac ccgctgcgcg cgcgcctgac cggaccggcg
     8581 gcgcgccggg aggggacaca ccactaggct ggcgcctgct cgttcgtcaa gagaggtacc
     8641 acgtcacgtg atcacccgac tgtccacgct gttcgtccgc accctgcgtg aggatcccgc
     8701 cgacgccgag gtcgccagcc accgctggct ggtccgcgcc ggctacatcc gccgggtggc
     8761 gcccggcatc tacagctggc tgccgctggg cctgaaggtg ctggccaagg tcgagcgcat
     8821 cgtccgcgaa gagatggacg cgctcggcgg ccaagaggtg cgctttcccg cgctgctgcc
     8881 gcgcgagccc tacgagacca cccatcgctg gaccgagtac ggtcccaacc tgtttcggct
     8941 caaggaccgc aagggcggcg actacctgct cggacccacc cacgaagaga tgttcaccct
     9001 gctggtgaag gacctgtaca gctcgtacaa ggacctgccg ct
//
LOCUS       DAOSUG010000049         9023 bp    DNA     linear   ENV 10-JUN-2024
DEFINITION  MAG TPA_asm: Micropruina sp. isolate AMR_MDS_2195
            NODE_4105_length_9023_cov_3.073483, whole genome shotgun sequence.
ACCESSION   DAOSUG010000049 DAOSUG010000000
VERSION     DAOSUG010000049.1
DBLINK      BioProject: PRJNA925477
            BioSample: SAMN32864262
            Sequence Read Archive: SRR4236673
KEYWORDS    WGS; ENV; Metagenome Assembled Genome; MAG; Third Party Data; TPA;
            TPA:assembly.
SOURCE      Micropruina sp. (wastewater metagenome)
  ORGANISM  Micropruina sp.
            Bacteria; Bacillati; Actinomycetota; Actinomycetes;
            Propionibacteriales; Nocardioidaceae; Micropruina.
REFERENCE   1  (bases 1 to 9023)
  AUTHORS   Abdulkadir,N., Saraiva,J.P., Zhang,J., Stolte,S., Gillor,O.,
            Harms,H. and Rocha,U.
  TITLE     Genome-centric analyses of 165 metagenomes show that mobile genetic
            elements are crucial for the transmission of antimicrobial
            resistance genes to pathogens in activated sludge and wastewater
  JOURNAL   Microbiol Spectr 12 (3), e0291823 (2024)
   PUBMED   38289113
REFERENCE   2  (bases 1 to 9023)
  AUTHORS   Abdulkadir,N.
  TITLE     Direct Submission
  JOURNAL   Submitted (24-MAR-2023) Environmental Microbiology, Helmholtz
            Center for Environmental Research -UFZ, Permoserstr 15, Leipzig
            04318, Germany
COMMENT     The annotation was added by the NCBI Prokaryotic Genome Annotation
            Pipeline (PGAP). Information about PGAP can be found here:
            https://www.ncbi.nlm.nih.gov/genome/annotation_prok/
            
            ##Genome-Assembly-Data-START##
            Assembly Method        :: SPAdes v. 3.11.1
            Genome Representation  :: Full
            Expected Final Version :: Yes
            Genome Coverage        :: Not Applicable
            Sequencing Technology  :: Illumina MiSeq
            ##Genome-Assembly-Data-END##
            
            ##Genome-Annotation-Data-START##
            Annotation Provider               :: NCBI
            Annotation Date                   :: 05/04/2023 04:57:10
            Annotation Pipeline               :: NCBI Prokaryotic Genome
                                                 Annotation Pipeline (PGAP)
            Annotation Method                 :: Best-placed reference protein
                                                 set; GeneMarkS-2+
            Annotation Software revision      :: 6.5
            Features Annotated                :: Gene; CDS; rRNA; tRNA; ncRNA
            Genes (total)                     :: 2,776
            CDSs (total)                      :: 2,750
            Genes (coding)                    :: 2,714
            CDSs (with protein)               :: 2,714
            Genes (RNA)                       :: 26
            tRNAs                             :: 24
            ncRNAs                            :: 2
            Pseudo Genes (total)              :: 36
            CDSs (without protein)            :: 36
            Pseudo Genes (ambiguous residues) :: 0 of 36
            Pseudo Genes (frameshifted)       :: 2 of 36
            Pseudo Genes (incomplete)         :: 32 of 36
            Pseudo Genes (internal stop)      :: 2 of 36
            ##Genome-Annotation-Data-END##
FEATURES             Location/Qualifiers
     source          1..9023
                     /organism="Micropruina sp."
                     /mol_type="genomic DNA"
                     /submitter_seqid="NODE_4105_length_9023_cov_3.073483"
                     /isolate="AMR_MDS_2195"
                     /isolation_source="Activated sludge of municipal
                     wastewater treatment plants"
                     /db_xref="taxon:2737536"
                     /environmental_sample
                     /geo_loc_name="Canada"
                     /lat_lon="49.25 N 123.1 W"
                     /collection_date="2015-06-16"
                     /metagenome_source="wastewater metagenome"
                     /note="metagenomic"
     gene            complement(<1..1704)
                     /gene="hrpA"
                     /locus_tag="PLF56_02770"
     CDS             complement(<1..1704)
                     /gene="hrpA"
                     /locus_tag="PLF56_02770"
                     /EC_number="3.6.4.13"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_013161178.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_function: GO:0004386 - helicase activity [Evidence
                     IEA];
                     GO_process: GO:0006139 - nucleobase-containing compound
                     metabolic process [Evidence IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="ATP-dependent RNA helicase HrpA"
                     /protein_id="HOQ52517.1"
                     /translation="MPVPDVKLAVDPALPIAARADDIVAAIREHQVVIVAGETGSGKT
                     TQLPKLCLAAGRTRIGHTQPRRIAARSLAERIAEEIGTPLGEVVGYQVRFTARTSRQT
                     RVKVMTDGILLAEIAHDRDLRRYDTLIIDEAHERSLNIDFLLGYLAQLLPRRPDLKLI
                     VTSATIDTARFSEHFGGAPIIEVSGRTYPVEVRWRPVIEQGTDRDVIDGICDAVTELM
                     LDGPDGDILVFCSGERDIRDAADAIGALDWPSMTRREVEILPLYARLSAHEQHRVFAR
                     HTGLRVVLATNVAETSLTVPGIRSVIDPGFARISRYSARTKVQRLPIEAISQASANQR
                     AGRCGRLGPGICIRLYSEADYDARPEFTEPEILRTNLASVILQMAQARLGDIARFPFV
                     EPPDAAQIGDGLRLLTELGAIESDRDRGLSLSKPGQAAQGASTSSTNGGGASTDRGLS
                     LSKPGGAAGEVSTSGRRRRGGRNDRVRLTPIGHQLARLPVDPRLGRMLVEAERQGCLR
                     EAQAVVAGLTVPDVRERPADKQEQADQLHRRFFSESPEQGKDAPRDPSDIAALLRLWR
                     YL"
     gene            complement(1740..2612)
                     /locus_tag="PLF56_02775"
     CDS             complement(1740..2612)
                     /locus_tag="PLF56_02775"
                     /inference="COORDINATES: protein motif:HMM:NF012791.2"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_function: GO:0032559 - adenyl ribonucleotide binding
                     [Evidence IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="universal stress protein"
                     /protein_id="HOQ52518.1"
                     /translation="MVKPYVGPPPILVGYDASDDAVQALEYAAKVAAATKTLLQLVYV
                     ADDTVVNSAWGVVFDGSEVQSTARRLLAEAATVAHSHGVTKRRIQTKVAVGTPVGVLT
                     QLSQHCSMVVVGRRANSGHTHQFSGSTAVGLAASVRCPLVVVAEAPERPQGPIGVALD
                     PAGPGTGALQWVLTNPVYAGQSVRVVSVCKAPQSRIFRSSVTQEQIDSAIAQTVKGQA
                     ALIKQVLADHFDPPAITTEVRYGSPVDELAAFSEGVSVLVLEPVVRFPTYSIGSTTRG
                     MMTYASCPVVLVKA"
     gene            complement(2650..3042)
                     /locus_tag="PLF56_02780"
     CDS             complement(2650..3042)
                     /locus_tag="PLF56_02780"
                     /inference="COORDINATES: protein motif:HMM:NF024304.2"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="pyridoxamine 5'-phosphate oxidase family
                     protein"
                     /protein_id="HOQ52519.1"
                     /translation="MAQYGHLTTLDAAECRLLLGTRTFGRVGWNSASGVQILPVSYGL
                     LGDTIVFRTQAGSPLAELVRPLEVSFQVDDLDDETSTGWSILVHGRSGAPVTRLPGNM
                     PDPWAPGERPLVIGITADHYAGRSIAAN"
     gene            complement(3064..5439)
                     /locus_tag="PLF56_02785"
     CDS             complement(3064..5439)
                     /locus_tag="PLF56_02785"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_013161179.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="phosphoketolase family protein"
                     /protein_id="HOQ52520.1"
                     /translation="MAANLPALTESELESVDAWWRANNYLTVGQIYLMANPLLREPLA
                     PEHIKPRLLGHWGTSTGLAFIYAHVSRLIRHTGQDCLYITGPGHGGPALVGASYLEGT
                     YTDAFPHITQDNEGLLKFFRQFSAPGGIPSHVSVTTPGSIHEGGELGYALIHAFGSVF
                     DNPDLLTIAVVGDGEAETGPLEGSWKGISFLNPDHDGAVLPVLHLNGAKIAAPTVLGR
                     KPHAEVRELLEGHGYEVIEVEGHDLPGMHYRFAEALGLAYERIKQIQQRAREGSGFTS
                     RPRWPMIILRSPKGWTGPHEVDGIQVEGTWRAHQVPLSGVRTNPAHLAQLEGWLKSYR
                     PEELFDDNGRPTALVTAANPDKIASMSGTPYANGGLLTEPLDLPDFRELAVEVEQRGG
                     ERIESTRKLGELMREAYRRNPHNFRLFCPDETNSNRLGAVFEVSDRAWMESLTDIDVK
                     LGPNGRVMEVLSEHNCHGWLEGYTLTGRHGLFATYEAFAMVSASQTIQHGKWLQEASH
                     LPWRADVPSLNVLLSSTAWRNDHNGFSHQGPGMLQVVLNGGAQHSRIYLPPDANCLLS
                     VADHCFRSVNYVNLIVQDKQPQPQWLSMPEAIAHCTKGASIWQWAGTPEDAGEPDIVL
                     ACAGDIPTLEAVAAAEIIKAKLPDIRLRVVNVVDLMTLMRPKDHPHGMSELYFKELFT
                     DNRDVVFAFHGYPGAIHQLVHGRPDADRFRVRGFIEQGTTTTPFDMVVRNRTSRYHLV
                     MDVLNNMKVTRRGSGDLMGFCEAQLARHESYIVEHLEDMPEVQDWKLGGPV"
     gene            complement(5699..6640)
                     /locus_tag="PLF56_02790"
     CDS             complement(5699..6640)
                     /locus_tag="PLF56_02790"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_015070322.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="hypothetical protein"
                     /protein_id="HOQ52521.1"
                     /translation="MDDPNNASEVHGFDLDRSTDEAWTDFTARLGDVIAHMDAGATLR
                     IGSMATEQEGTSPFVLFRCLPNERLVAEAAGNAALSEEFQLAPHQLEALEELGWNPPN
                     VDSSHPCENYWMPGDQENALDLARRAGDVLRSVYGVPHPAFLAPDQLAEILTPPGVRE
                     APATTFESDELIAHLVTGKTQLDRLIKAELTGLLGHSPLQDADGDFALRVGSTMVFVR
                     ATTDAQEVLVFSAVVHDVEGRSRAMEVLSDLNTEARFVRFMLIRDRVFVSLSVFTNPF
                     VPAHLHQALKAVSVVADEIDEHLASKLRGRTTFAGDA"
     gene            complement(6721..7656)
                     /locus_tag="PLF56_02795"
     CDS             complement(6721..7656)
                     /locus_tag="PLF56_02795"
                     /inference="COORDINATES: protein motif:HMM:NF015509.2"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_process: GO:0055085 - transmembrane transport [Evidence
                     IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="AEC family transporter"
                     /protein_id="HOQ52522.1"
                     /translation="MQGALAGFFTIGAIVFTGVALAHFRVLGELHRALLNQLAFLVAS
                     PALIFSVVAGADLGHLFSHTLTVSLLAIAAAASRYLVASRALRGRFDARTVIGTLCAC
                     YTNAGNLGLPIAAAILGDMSWMAPIMLVQVGLLQPLALALLDTRSARHAGRPMPWYTY
                     LTLPLRNPITTAVLLGVAVNLFGVTVPALLMAPIDMVGAVAVPAMLLAFGVSLRLDPM
                     SGRGEHVALVWLVLIIKLVLMPLLAWGLGAYLFGLSGHELLAVTVIAALPSAQNIYVI
                     AARYEVGELLARDSIFTSTLLSVPVITLIAGLLGG"
     gene            7755..>9023
                     /locus_tag="PLF56_02800"
     CDS             7755..>9023
                     /locus_tag="PLF56_02800"
                     /EC_number="2.3.1.-"
                     /inference="COORDINATES: protein motif:HMM:NF013884.2"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_function: GO:0016747 - acyltransferase activity,
                     transferring groups other than amino-acyl groups [Evidence
                     IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="acyltransferase"
                     /protein_id="HOQ52523.1"
                     /translation="MNARNHLIDLARAMSILVVVTFHCLLYQLQVVDGRPQMVPWAPQ
                     PHAAWWTASWFIMIIPLFFIAGGFAHALVVDKLRAQQRGYTYYLASRAHRLVGPLLLF
                     VGFCTVVSTAGAWFGPTALSIGLSVQFAQLLWFLAVYLVIVGVAPFMVSLHDRVPWLP
                     LLVLGLVAVGVDAWSFAVGNFEIRYLNLVTVWPLCHQLGIAYHRGWFRQGPQWVPWLV
                     ILAGAAVIPVLIFGFGYPGSSIGLGDIPIANVLPPTVAMAVLGAGQAAVLGLLQRAGV
                     ARRLRARTERGLAVANALAVTTYLWHIPCIVVAGGLLFALSRLAPGATDVLMSQPAVV
                     LLTWAVIVLVVPWIGRVEYALIPRLGDTPGRGVLPAYVLLVAGTGLVWRFGTVLHPAE
                     PASALGLLLLGAGMTWLARAAGAPAPDAART"
BASE COUNT         1402 a         3086 c         3091 g         1444 t
ORIGIN      
        1 caggtaacgc cacaaccgca gcagcgcggc gatatcggac gggtcgcgcg gggcgtcctt
       61 gccctgctcg ggcgactcgc tgaagaaccg gcgatgcagc tggtcggcct gttcctgctt
      121 gtcagcaggc ctttcccgga cgtcgggcac ggtcagcccg gccaccaccg cctgcgcctc
      181 gcgcaggcaa ccctggcgtt cggcctcgac cagcatccgg cccagccgtg ggtcgaccgg
      241 cagccgagcc agctggtgcc cgatcggggt cagccggacg cggtcgttgc gtccgccccg
      301 tcgccggcgg ccgctggtcg agacctcacc agccgccccg ccgggtttcg acaagctcaa
      361 ccctcggtca gtcgaggccc cgccaccgtt ggtcgagctt gtcgaggccc cctgggcagc
      421 ctgaccgggt ttcgacaagc tcaaccctcg atcgcggtcg gattcgatcg ctcccagttc
      481 ggtgagcagc cgcagtccgt cgccgatctg ggcggcgtcc gggggttcga cgaacgggaa
      541 gcgggcgatg tcgccgagcc gggcctgggc catctgcagg atgaccgagg ccaggttggt
      601 gcgcaggatc tcgggctcgg tgaactcggg ccgggcgtcg tagtccgcct ccgagtagag
      661 ccggatgcag atgccggggc cgagccggcc gcagcggccg gcgcgctggt tggccgaggc
      721 ctgcgagatc gcctcgatcg gcagccgctg caccttggtg cgcgccgagt agcgcgagat
      781 gcgggcgaac cccgggtcga tcaccgagcg gatgcccggc acggtcagcg aggtctcggc
      841 gacgttggtg gccaacacca cccgcagccc cgtatgccgg gcgaacaccc gatgctgttc
      901 gtgggccgag agccgcgcgt acagcggcag gatctccacc tcgcgccggg tcatcgacgg
      961 ccagtccagc gcaccgatcg cgtccgcagc gtcgcggatg tcgcgctcac ccgagcagaa
     1021 caccagaatg tcgccgtcgg ggccgtccag catcaactcg gtcacggcgt cgcagatgcc
     1081 gtcgatcacg tcgcggtcgg tgccctgctc gatcaccggg cgccagcgca cctcgaccgg
     1141 gtaggtgcgt ccggacacct cgatgatcgg ggccccgccg aaatgctcgc tgaaccgggc
     1201 ggtgtcgatg gtggccgagg tgacgatcag cttcaggtcc ggacgccgtg gcaacagttg
     1261 ggccaggtaa ccgagcagaa agtcgatgtt gaggctgcgt tcgtgcgcct cgtcgatgat
     1321 cagggtgtcg taacgacgca gatcgcgatc gtgcgcgatc tcggccagca gaatgccgtc
     1381 ggtcatcacc ttcacccggg tctgccggct ggtcctggcc gtgaaccgca cctggtagcc
     1441 gaccacctcg cccagcggcg tgccgatctc ttcggcgatg cgttcggcga ggctgcgggc
     1501 ggcgatgcga cgtggttgag tgtggccgat gcgcgtccgt ccggcggcga ggcacagctt
     1561 gggcagctgg gtggtcttgc ccgaaccggt ctcgcccgcc acgatcacca cctggtgctc
     1621 gcgaatcgcc gccacgatgt cgtcggcccg cgcggcgatc ggcagggccg gatcgacggc
     1681 gagcttcacg tcaggtacgg gcacgacgct caagcctaca aggcatcacc ggcccgggct
     1741 caggccttga ccagcaccac cggacaactg gcgtaggtca tcatgccccg cgtggtgctg
     1801 ccgatcgaat aggtgggaaa ccgcaccacc ggttcgagca ccagcaccga caccccctcg
     1861 ctgaacgccg caagttcgtc caccggcgag ccgtagcgca cctcggtggt gatcgccggc
     1921 ggatcgaagt ggtcggcgag tacctgtttg atcagggccg cctggccctt gaccgtctgc
     1981 gcgatcgccg agtcgatctg ctcctgggtg acgctgctgc ggaagatgcg gctctgtggc
     2041 gccttgcaga cgctcacgac ccgtacgctc tgaccggcgt agaccggatt ggtcagcacc
     2101 cactgcagcg caccggtacc cggaccggcc gggtcgagcg caactccgat cgggccctgc
     2161 gggcgttccg gcgcctcggc caccaccacg agcgggcagc gcacggacgc ggccaaaccc
     2221 accgcggtcg aacccgagaa ctggtgggtg tggccgctgt tggcgcgccg gcccaccacc
     2281 accatggaac agtgctggct gagctgggtc agcacgccca ccggggtgcc gaccgcgacc
     2341 ttggtctgga tgcgacgttt ggtgacgccg tgcgaatggg cgaccgttgc cgcttcggcg
     2401 agcagtcgtc gagcggtgct ctgcacctca ctgccgtcga agaccacccc ccaggcgctg
     2461 ttgaccacgg tgtcgtcggc gacgtagacc aactgcaaca gggtcttggt ggcggcggcc
     2521 accttggcgg cgtactcgag cgcctgcacg gcgtcgtcgg acgcgtcgta gccgaccagg
     2581 atcggtggtg gaccgacgta cggcttgacc atggctcgct tcctcttcga tgacgaacgg
     2641 tcgatggcgt cagttggctg cgatggaacg tccggcgtag tgatcggcgg tgatgccgat
     2701 caccaggggc cgctcgcccg gtgcccacgg gtcgggcatg ttgcccggca ggcgggtcac
     2761 cggggcaccg gacctaccgt gcaccagaat gctccacccc gtggacgtct cgtcgtccag
     2821 atcgtcgacc tggaacgaca cttcgagcgg acgcaccaac tcggccaacg gtgagccggc
     2881 ctgggtgcga aacacgatcg tgtcaccgag caggccgtag ctcaccggca ggatctgcac
     2941 gcccgaggcc gagttccagc cgacccgacc gaaggtgcgg gtacccaaca gcagccgaca
     3001 ctccgcagca tcaagtgtgg tgaggtgccc gtactgcgcc atagggcaga ccttacccac
     3061 atttcacacg gggcccccca gcttccagtc ctgcacctcc ggcatgtctt ccaggtgctc
     3121 gacgatgtag ctctcgtggc gggccagctg ggcctcgcag aaccccatca ggtcccccga
     3181 gccgcgcctg gtcaccttca tgttgttcag cacgtccatc accaggtggt agcgcgaggt
     3241 gcggttgcgc accaccatgt cgaacggcgt ggtcgtggtg ccctgctcga tgaagccgcg
     3301 cacccggaag cggtcggcgt ccggacgtcc gtgcacgagt tggtggatcg cccccggata
     3361 gccatggaac gcgaacacca cgtcgcggtt gtcggtgaac agctccttga agtacagctc
     3421 gctcatgccg tgcgggtggt ccttgggccg catcagggtc atcaggtcga ccacgttcac
     3481 cacccgcagc cgaatgtcgg gcagcttggc cttgatgatc tcggcggccg ccaccgcttc
     3541 cagcgtcgga atgtcaccgg cgcaggccag cacgatgtcg ggctcaccgg cgtcttcggg
     3601 cgtgccggcc cactgccaga tggacgcacc cttggtgcag tgcgcgatcg cctcgggcat
     3661 gctcagccac tgcggctgcg gctgcttgtc ttgcacgatc aggttgacgt agttgacgct
     3721 gcggaaacag tggtcggcca cgctgagcag gcagttcgcg tcgggcggca ggtagatgcg
     3781 ggaatgttgc gcaccgccgt tcagcaccac ctgcagcatg cccgggccct gatggctgaa
     3841 accgttgtgg tcgttgcgcc aagcggtcga gctgagcagc acgttgagac tgggcacgtc
     3901 ggcgcgccac ggcagatgcg aggcctcctg cagccacttg ccgtgctgga tggtctggct
     3961 ggccgacacc atggcgaacg cctcataggt ggcgaacaac ccgtgccgcc cggtcagggt
     4021 gtagccctcg agccagccgt gacagttgtg ttcgctgagc acctccatca cccggccgtt
     4081 ggggcccagc ttgacgtcga tgtcggtcaa cgactccatc caggcccggt cgctgacctc
     4141 gaacaccgca ccgagccggt tgctgttggt ctcatccgga cagaacagcc gaaagttgtg
     4201 cgggttgcgc cggtaggcct cgcgcatcaa ctcaccgagc ttgcgggtgg attcgatgcg
     4261 ttcgccgccc cgctgctcga cctcgaccgc aagttcacgg aagtcgggaa ggtcgagcgg
     4321 ctcggtcaac aggccgccgt tggcgtaggg ggtgcccgac atggacgcga tcttgtcggg
     4381 gttggcggcc gtgaccagcg cggtggggcg tccgttgtcg tcgaacagtt cctcgggacg
     4441 gtacgacttc agccacccct ccaactgcgc caggtgcgcc ggattcgttc gcacgcccga
     4501 cagcggcacc tgatgcgccc gccaggtgcc ctcgacctga atgccgtcga cctcgtgcgg
     4561 ccccgtccaa cccttggggc tgcgcagaat gatcatcggc cagcgcggac gcgacgtgaa
     4621 cccggagccc tcacgggccc gctgctgaat ctgcttgatg cgctcgtagg ccagccccag
     4681 cgcctcggcg aagcggtagt gcatgccggg aagatcgtgg ccctcgacct cgatcacctc
     4741 atagccatgc ccttcgagca gttcgcgcac ctcggcgtgc ggcttgcggc ccagcacggt
     4801 gggcgcggcg atcttggcgc cgttgaggtg cagcacgggc agcacggccc cgtcgtggtc
     4861 gggattcagg aacgagatgc ccttccagga accctccagg ggcccggtct cggcctcgcc
     4921 gtcaccgacc acggcgatgg tcagcagatc ggggttgtcg aacaccgaac cgaacgcgtg
     4981 gatcagtgcg tacccgagtt caccgccctc gtggatcgaa cccggggtgg tcaccgagac
     5041 gtggctggga atgccgccgg gcgccgagaa ctgccggaag aacttgagca ggccctcgtt
     5101 gtcttgggtg atgtggggaa aggcgtcggt gtaggtgccc tccagatagg acgccccgac
     5161 cagcgccggg ccgccgtgac ccggcccggt gatgtacagg cagtcctgac cggtgtgcct
     5221 gatcagccgt gacacatgtg catagatgaa cgccaggccg gtgctggtgc cccagtggcc
     5281 gagcaaccgg ggcttgatgt gctcaggcgc cagcggttca cgaagcaggg ggttggccat
     5341 caggtagatc tgcccgacgg tcaggtagtt gttggcccgc caccaggcgt ccacgctctc
     5401 gagttcggat tcggtcagtg caggaaggtt tgccgccatg ggcccacgct agccgtaggg
     5461 tctgacaagc aacacccgcc gacagcgctt tttggtgctg atccggggca gagatcgaca
     5521 ggactggtgt cgactgcggg caccgaggcc gccgctagct caccgctgat cgagccctgt
     5581 ccagagcccg ccgcccggca tgccacccga gccggccgcc caagcccgct tcgcgagccc
     5641 cgcaacgtgc gccgcccgga agtgccccgc gtcagggcgt ggacggcctc ggcgacgctc
     5701 aggcgtcgcc ggcgaacgtg gtgcgacccc gcagcttgga cgccaggtgc tcgtcgatct
     5761 cgtccgccac caccgacacg gccttcagcg cctggtgcaa atgagcgggc acaaaaggat
     5821 tggtgaagac cgacagcgag acgaacaccc ggtcgcgaat cagcatgaaa cgcacgaagc
     5881 gggcctcggt gttcaggtca ctgagcacct ccatggcacg gctgcggccc tccacgtcgt
     5941 gcaccaccgc cgagaacacc agcacttcct gggcgtcggt ggtcgcccgc acgaacacca
     6001 tggtcgaacc gacccgcagg gcgaagtcac cgtcggcgtc ctgcaggggc gaatggccga
     6061 gcagcccggt cagctcggcc ttgatcagcc ggtcgagctg cgtcttgccg gtcaccagat
     6121 gggcgatcag ttcgtcggat tcaaaggtcg tggccggagc ctccctgacc ccgggcgggg
     6181 tcaggatctc agccagttgg tcgggcgcca ggaaggccgg gtgaggcacc ccgtacacgc
     6241 tgcgcaacac gtcacccgcg cgccgggcca ggtcgagggc gttctcctgg tcgcccggca
     6301 tccagtagtt ctcacacggg tggctcgagt cgacgttggg cgggttccag ccgagctctt
     6361 cgagcgcctc caactggtgc ggggcaagct ggaactcctc gctcagggca gcattacccg
     6421 cagcctccgc gaccaggcgc tcgttcggca ggcagcggaa caacacgaag ggcgaggtgc
     6481 cctcctgctc ggtcgccatc gatccgatac gcaaggtggc gcccgcgtcc atgtgcgcga
     6541 tcacgtcgcc cagccgggcg gtgaagtccg tccacgcctc gtccgtgctg cggtcgaggt
     6601 cgaagccgtg cacctcgctg gcgttgttcg ggtcgtccac gcgtccaacc taaacgttcg
     6661 gcatggccga ccgcgaccct gcaccgccaa catcgccaag cgcaaggcat ccgccgcggg
     6721 tcacccgccc agcaggcctg cgatcagcgt gatcaccggc accgacagca gggtcgaggt
     6781 gaagatcgag tcgcgcgcca gcagttcgcc cacctcgtag cgcgccgcga tcacatagat
     6841 gttctgagcg gacggcagcg cggcgatcac cgtgaccgcc aacagctcat gtccgctcag
     6901 gccgaagagg taggccccca acccccaggc gagcaggggc atcagcacca gcttgatgat
     6961 caggaccaac cagaccagcg caacgtgctc gccccgtccg gacatcgggt cgagccgcag
     7021 cgacacgccg aacgccagca gcatcgcggg caccgcgaca gcgccgacca tgtcgatcgg
     7081 ggccatcagc agcgcgggaa cggtcacacc gaacaggttg acggcgaccc ccagcagcac
     7141 cgcggtggtg atcggattgc gcaacggcaa ggtcagatag gtgtaccagg gcatcggacg
     7201 tccggcgtgc cgcgcgctgc gggtgtcgag cagcgccaac gccagcggct gcagcagacc
     7261 gacctgcacc agcatgatcg gcgccatcca gctcatgtcg ccgaggatcg ccgcggcgat
     7321 cggcaggccc aggttgccgg cattggtgta gcaggcgcac agcgtaccga tcacagtgcg
     7381 ggcgtcgaag cgtccgcgca gcgcccgtga cgcgaccagg tagcgcgatg cggcagcggc
     7441 aatggccagc agcgacaccg tgagggtgtg cgagaacagg tggccgaggt cggcccctgc
     7501 caccaccgag aagatcagtg ccggggacgc caccagaaac gccagctggt tcaacagcgc
     7561 ccggtgcagc tcgccgagca cccgaaagtg cgccagcgca acaccggtga acacgatcgc
     7621 tccgatcgtg aagaagccgg ccagcgcacc ctgcacccgg gccagcctat gcatccggac
     7681 ccgggagcgg ggggctgtcc ggggcagcgc tcgcggcccc ggtcggcggt gggcgcggcg
     7741 tacgatgacg gccgatgaac gcccgcaacc acctgatcga cctggccagg gccatgtcga
     7801 tcctcgtggt cgtcacgttt cactgcttgc tgtaccaact gcaggtggtg gacgggcggc
     7861 cgcagatggt tccgtgggcg ccccagccgc acgccgcgtg gtggacggcc agctggttca
     7921 tcatgatcat cccgctgttc ttcatcgcag gcgggttcgc gcatgccctg gtggtcgaca
     7981 agctgcgggc gcagcaacgc ggctacacct actacctcgc cagccgcgca caccggctgg
     8041 tggggccgtt gctgttgttc gtcggcttct gcacggtggt gagcacagcg ggggcatggt
     8101 tcgggccgac cgcgctgagc atcggtctca gcgttcagtt cgcccagttg ctgtggtttc
     8161 tggccgtcta cctggtgatc gtcggggtgg cgccgttcat ggtcagcctc cacgatcggg
     8221 tgccctggtt gccgctgctg gtactcggcc tggtggccgt cggcgtggac gcctggtcat
     8281 tcgcggtcgg taatttcgag atccgctacc tgaacctggt gacggtctgg ccgttgtgcc
     8341 accagctggg catcgcgtac caccggggct ggttccggca ggggccgcag tgggtgccgt
     8401 ggctggtgat cctcgctggg gcggcggtga ttccggtgct gatcttcggg ttcggctacc
     8461 ccggctcgtc catcgggctg ggtgacattc cgatcgccaa cgtgttgccg ccgactgttg
     8521 caatggccgt gctgggcgcc gggcaggccg ccgttctggg gctgctgcaa cgcgcgggcg
     8581 tggcgcggcg gcttcgggcg cgcaccgagc gggggctggc cgtggccaac gccctggctg
     8641 tgaccaccta cctgtggcac atcccgtgca tcgtggtcgc cggcggcctg cttttcgctc
     8701 tctcgcggtt ggcgcccggc gcgaccgacg tactgatgtc gcagccggcg gtggtgctgc
     8761 tcacctgggc ggtcatcgtg ctggtcgtcc cgtggatcgg acgcgtcgag tacgccctga
     8821 tcccccggct gggcgatact cccggacgcg gcgtgctgcc cgcgtacgtg ttgctggtgg
     8881 cgggtaccgg cctggtctgg cgcttcggca cggtgctgca ccccgccgag ccggcctcgg
     8941 ctctcgggct gctgctgctc ggcgccggca tgacctggct ggcccgcgcc gccggggccc
     9001 cggcacccga tgccgcccgc acc
//
LOCUS       DAOSUG010000050         8969 bp    DNA     linear   ENV 10-JUN-2024
DEFINITION  MAG TPA_asm: Micropruina sp. isolate AMR_MDS_2195
            NODE_4131_length_8969_cov_4.815571, whole genome shotgun sequence.
ACCESSION   DAOSUG010000050 DAOSUG010000000
VERSION     DAOSUG010000050.1
DBLINK      BioProject: PRJNA925477
            BioSample: SAMN32864262
            Sequence Read Archive: SRR4236673
KEYWORDS    WGS; ENV; Metagenome Assembled Genome; MAG; Third Party Data; TPA;
            TPA:assembly.
SOURCE      Micropruina sp. (wastewater metagenome)
  ORGANISM  Micropruina sp.
            Bacteria; Bacillati; Actinomycetota; Actinomycetes;
            Propionibacteriales; Nocardioidaceae; Micropruina.
REFERENCE   1  (bases 1 to 8969)
  AUTHORS   Abdulkadir,N., Saraiva,J.P., Zhang,J., Stolte,S., Gillor,O.,
            Harms,H. and Rocha,U.
  TITLE     Genome-centric analyses of 165 metagenomes show that mobile genetic
            elements are crucial for the transmission of antimicrobial
            resistance genes to pathogens in activated sludge and wastewater
  JOURNAL   Microbiol Spectr 12 (3), e0291823 (2024)
   PUBMED   38289113
REFERENCE   2  (bases 1 to 8969)
  AUTHORS   Abdulkadir,N.
  TITLE     Direct Submission
  JOURNAL   Submitted (24-MAR-2023) Environmental Microbiology, Helmholtz
            Center for Environmental Research -UFZ, Permoserstr 15, Leipzig
            04318, Germany
COMMENT     The annotation was added by the NCBI Prokaryotic Genome Annotation
            Pipeline (PGAP). Information about PGAP can be found here:
            https://www.ncbi.nlm.nih.gov/genome/annotation_prok/
            
            ##Genome-Assembly-Data-START##
            Assembly Method        :: SPAdes v. 3.11.1
            Genome Representation  :: Full
            Expected Final Version :: Yes
            Genome Coverage        :: Not Applicable
            Sequencing Technology  :: Illumina MiSeq
            ##Genome-Assembly-Data-END##
            
            ##Genome-Annotation-Data-START##
            Annotation Provider               :: NCBI
            Annotation Date                   :: 05/04/2023 04:57:10
            Annotation Pipeline               :: NCBI Prokaryotic Genome
                                                 Annotation Pipeline (PGAP)
            Annotation Method                 :: Best-placed reference protein
                                                 set; GeneMarkS-2+
            Annotation Software revision      :: 6.5
            Features Annotated                :: Gene; CDS; rRNA; tRNA; ncRNA
            Genes (total)                     :: 2,776
            CDSs (total)                      :: 2,750
            Genes (coding)                    :: 2,714
            CDSs (with protein)               :: 2,714
            Genes (RNA)                       :: 26
            tRNAs                             :: 24
            ncRNAs                            :: 2
            Pseudo Genes (total)              :: 36
            CDSs (without protein)            :: 36
            Pseudo Genes (ambiguous residues) :: 0 of 36
            Pseudo Genes (frameshifted)       :: 2 of 36
            Pseudo Genes (incomplete)         :: 32 of 36
            Pseudo Genes (internal stop)      :: 2 of 36
            ##Genome-Annotation-Data-END##
FEATURES             Location/Qualifiers
     source          1..8969
                     /organism="Micropruina sp."
                     /mol_type="genomic DNA"
                     /submitter_seqid="NODE_4131_length_8969_cov_4.815571"
                     /isolate="AMR_MDS_2195"
                     /isolation_source="Activated sludge of municipal
                     wastewater treatment plants"
                     /db_xref="taxon:2737536"
                     /environmental_sample
                     /geo_loc_name="Canada"
                     /lat_lon="49.25 N 123.1 W"
                     /collection_date="2015-06-16"
                     /metagenome_source="wastewater metagenome"
                     /note="metagenomic"
     gene            <1..283
                     /locus_tag="PLF56_02805"
     CDS             <1..283
                     /locus_tag="PLF56_02805"
                     /inference="COORDINATES: protein motif:HMM:NF012371.2"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=2
                     /transl_table=11
                     /product="serine hydrolase"
                     /protein_id="HOQ52524.1"
                     /translation="PGATGYGLGFGCGNGWVGHSGEVPGYSTSMFYDTINDYSVIVQT
                     NSDIASGSCEASPTLQDNPADLPCSAPSVRVLVALTDVLGNRFEPPARK"
     gene            744..2528
                     /locus_tag="PLF56_02810"
     CDS             744..2528
                     /locus_tag="PLF56_02810"
                     /inference="COORDINATES: protein motif:HMM:NF012356.2"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_function: GO:0003824 - catalytic activity [Evidence
                     IEA];
                     GO_process: GO:0005975 - carbohydrate metabolic process
                     [Evidence IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="alpha-amylase family glycosyl hydrolase"
                     /protein_id="HOQ52525.1"
                     /translation="MGAVPHHGGVTFRVWAPNARTVHVAGDFSRWRTGRVPMAPDHSR
                     SGTWSAFVAQAGVGSQYKFLIRRGGPYRWRIDPFAREVTHSRGNAVVFDPKSIQWHER
                     PFTMPGWDELVLYELHVGTFATHGAEPGTFDTAIGRLDHLRRLGVNAVEVMPPFSFGG
                     PRSWGYNPSHLFAIETSYGGPRAFARFVDAAHQRGIAVILDVVHNHLGPTDLDLWRFD
                     GTSSRGYGGQYFYNDRRALTPWGATRPNYERKQVRNFLRDSAVMWLEEFRVDGLRFDG
                     TNFIRSRWGNTQHRPDRIAGGHRYLAATTVDIRARQPWKLLIAEDMQRDPVVTAPAEQ
                     GGLGFHSQWDADFVHPVRHALETPWDADRNTTAVARAVTGHYLRAAHERVIFTESHDE
                     VANGRSRLPETINPGAADSWPARKRAALGLALVLTSAGMPLLFQGQEFCEDRWFDDRT
                     PLSWNKTQRHSGFLHLARQLIRLRRNLDGHTAGLRGPHTALLRVDDQRKVLAYHRWWQ
                     GGPGDDVIVAVNLSTEVIHGYRVGLPRAGRWQLRCNSDAPIFGDDFGAAHASDLNADC
                     EPWDGRPFSAEVTIGPYAALVYSQDEGP"
     gene            complement(2557..4188)
                     /locus_tag="PLF56_02815"
     CDS             complement(2557..4188)
                     /locus_tag="PLF56_02815"
                     /inference="COORDINATES: protein motif:HMM:NF012371.2"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="serine hydrolase"
                     /protein_id="HOQ52526.1"
                     /translation="MPAPSQIATPAAVGRRTLLRGVGLGGAAAAAAAGLSACTGPSAP
                     AATVSPSGQASGVPSSADVIDAAAVQRGVDALPGIIVRYLQQYGVPGLAIAIVHEGQV
                     RYLEGFGLREVGKPDKPDADTVFQLASVSKPISATVVAAAMTKKLPTLGWDDPVQKAL
                     PDFTLSDAWTGSHVTVADMFAHRSGLPDHSGNLLEGLGYDRATILAKHRFYPLKRFRD
                     NYAYTNYGITAGAEAIAVSSGLKWEELAEQVLFGPLGMKDTSYRFADLQSRTNRAAMH
                     VQVDGTWVPNLKVDTDAQAPAGSSSSSIRDLATWLTMLLANGKPVIDEDQLQRIWLPA
                     VVRPGVPPIGKSAGFYGMGWIVSYEPTGELRVNHSGGFGLGAATTVTVYPTHQLGIAV
                     ITNGPPIGLPEAVGVEFYDHVRYGRSTEADWVAKIGPFVMPEETEDNKKYSVAATNPT
                     RGRSNAAYVGRYSSSFYGSLAVSDRSGTLHVTAGPAKMQFDLQHYSGDEFFFKTVGED
                     ETGLSGATFTTSGGRAAALTIGAWNHEGLGRFVRS"
     gene            complement(4301..5104)
                     /locus_tag="PLF56_02820"
     CDS             complement(4301..5104)
                     /locus_tag="PLF56_02820"
                     /EC_number="3.1.-.-"
                     /inference="COORDINATES: protein motif:HMM:NF024863.2"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_function: GO:0016788 - hydrolase activity, acting on
                     ester bonds [Evidence IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="SGNH/GDSL hydrolase family protein"
                     /protein_id="HOQ52527.1"
                     /translation="MSRLRRVVATLIAAVALFANAIAPADAAVPRHRAPRGYVALGDS
                     YAAGLGAAAPKDSCGRSSFGYPSVIAKVARLDLTLAACSGATMADVARTQLGQVTDSA
                     DFVTVQAGGNDIGFAPVLALCAQPASDDACAAAVAQGRAYLEGGFGANATALFAAVRD
                     RAPEATMVVVGYPRLFSGTDCSPLVEFSATEQKLINRTIDELNDQLADAARQVGARFA
                     NAESAFNSHAWCARVPWITGPAGPVAAFHPNVLGQLLGYAPVVGRKLLG"
     gene            complement(5215..5973)
                     /locus_tag="PLF56_02825"
     CDS             complement(5215..5973)
                     /locus_tag="PLF56_02825"
                     /inference="COORDINATES: protein motif:HMM:NF020916.2"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="polyphosphate polymerase domain-containing
                     protein"
                     /protein_id="HOQ52528.1"
                     /translation="MITTYPALDTIGLDELVERGSLLTRVDRKYALTAADAGMAVELV
                     DPATTRVLAIDGVPNLGYSSVYLDTPGLDTFLLAARQRRRRFKVRTRTYESNGASFLE
                     VKTRQHRHTVKDRLPGTHLVDGRLSDEGAAFVRAALRRAGVRSAPVEDLSASLRVDYR
                     RVTLFHEPTATRVTIDSGLTWWDVRTGAWLSLPDLSIIETKSTGRATGMDRLLWSLGH
                     RESSVSKYATALAAMHPHLPSNKWRRLLREHFGS"
     gene            6245..6862
                     /locus_tag="PLF56_02830"
     CDS             6245..6862
                     /locus_tag="PLF56_02830"
                     /inference="COORDINATES: protein motif:HMM:NF012514.2"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_function: GO:0016787 - hydrolase activity [Evidence
                     IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="NUDIX domain-containing protein"
                     /protein_id="HOQ52529.1"
                     /translation="MEVDHAGHRCGEPDQGLLLADDLDLAGGAVQVDRVVLVADPQCG
                     RARSARLRDVPVDRSAIRVKAMLIAPNGGRTAHAVSVHAPTLENPRGFHRLIGGSVEL
                     GERHLDTIRREITEELGAAVRDLVLLDTVENIVTIDGVIGHEIVFLCTGQLEPQPPAA
                     GATLTEIDGSVVPVVWRSISGEGETVPLYPSAAEWWVTHVATGLP"
     gene            6867..7814
                     /locus_tag="PLF56_02835"
     CDS             6867..7814
                     /locus_tag="PLF56_02835"
                     /inference="COORDINATES: protein motif:HMM:NF024676.2"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="WYL domain-containing protein"
                     /protein_id="HOQ52530.1"
                     /translation="MDDSPTARALRTLEAIHNRPGISAPELADRLGVTERAVRRYVGT
                     LRDAGVPVTGTAGRYGGYQIGRGVRLPPVAFTGTEALALVMAVVESRSGAPAGTPAGE
                     GVGKLLRALPEAVARSARQLWQHTAAAPSDFPRPDPGITGGLIEAVAAGRRTRLGYRS
                     ASGHEWTGEVDPWAVVVRRGLWYLLCANATAEVRTYRVDRVTAVEVLGTPADVPADLD
                     AAAALERGLSQGWRHPTRVRFADPVERVRDWLPGPMGDLVPDGDGCLLTGTTNNPDMY
                     AGEWLARVELPFVVEGGDELIAAMHRLVARLHAALPAQG"
     gene            complement(7829..8488)
                     /locus_tag="PLF56_02840"
     CDS             complement(7829..8488)
                     /locus_tag="PLF56_02840"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_020390649.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="EcsC family protein"
                     /protein_id="HOQ52531.1"
                     /translation="MSSDEPSKTIVQRAPEVAGGALRAILEFAIEGRGSIPGAKSAAA
                     KSLQSRGEHEAAVDSVIRQHVGLAGAQGFLTNLGGLITVPVALPANLAGLAVVQMRMI
                     AAIAHLRGYDVDDRRMRVALTLAMLGEDEVRKQVSSGKLPATPMVVATAPVFDPALDR
                     HVSERVFGDLGTKMAGKHVVVQVAKRIPLVGGGVGAAADSYFTYALGQYARREFVRRR
                     G"
     gene            8604..>8969
                     /locus_tag="PLF56_02845"
     CDS             8604..>8969
                     /locus_tag="PLF56_02845"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_013865983.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="leucyl/phenylalanyl-tRNA--protein transferase"
                     /protein_id="HOQ52532.1"
                     /translation="MSRPGIFGPSRDWPAQDLIGLSDEIDPDLVVLAYRCGVFPMPLH
                     NSWFPARMGWWSPMRRGVLLPDGLRVTRSLRKSAHRYTTTVDAAFEAVLARCADPGRP
                     HGWIDDDIVAVFTDLHRRGV"
BASE COUNT         1315 a         3130 c         3152 g         1372 t
ORIGIN      
        1 gccgggtgcg accggttacg gactcggctt cggctgcggc aacggctggg tggggcacag
       61 cggcgaggtg ccggggtaca gcaccagcat gttctacgac acgatcaacg actactcggt
      121 gatcgtgcag acgaacagcg acatcgcgtc cggttcgtgc gaggcctcgc cgacgctgca
      181 ggacaacccg gccgacctac cctgctcggc accgtccgtc cgggtgttgg tggcgctgac
      241 cgatgtgttg ggcaatcggt tcgagccgcc tgcgcggaag tgagcagccg cctggtgctg
      301 tggaggctgg aacccgttgg tcgaatggtc agggccgaac cgcgccgctc ttaacgtcga
      361 ggtcttccag atggtttggt gtgagaaccc ccatcctcga aagacctcga ccctcacccc
      421 ggcaccgacg cgccggacac catcacaccc cgcctacacc cccgattgtg atgagccgga
      481 ttggtcatgt tcgtaggcgc aagtctgatc acgccgtgga cgattcggcg ggcgatgttg
      541 gcgaggagcc ggcggttggt gccccccagg gcgtcccact tcgaagcccg ccccgagggc
      601 gacggcaact gagagcgtcg ctgcgggggc gacacgaaac atccggcgcc cgtgggccgg
      661 ctcaccagcc gtcgccgccg aaaagcgtcg gggctcaccc gtagtgtttc agcgtgtctg
      721 tcgctccgtc ccctcatgcc ggcatggggg ccgtccccca tcacggtggg gtgactttcc
      781 gggtctgggc acccaacgct cgcacggtgc acgtcgcggg cgacttcagt cgttggcgca
      841 ccggccgggt gccgatggcc cccgatcact cgcggtcggg tacctggtcg gcgttcgtgg
      901 cccaggccgg cgtgggttcg cagtacaagt tcctgatccg gcggggtggg ccctaccgat
      961 ggcgcatcga cccgttcgct cgcgaagtca cccacagccg tggcaatgcc gtggtgttcg
     1021 atccgaagtc gatccagtgg cacgagcgtc cgttcacgat gccgggctgg gacgagctgg
     1081 tgctctacga actgcacgtc ggcaccttcg ccacccacgg tgccgagccc ggcaccttcg
     1141 acaccgcgat cggacgcctc gaccacctgc gccggctggg cgtgaacgcg gtcgaggtga
     1201 tgccaccgtt cagcttcggc ggtccgcgca gttggggcta caacccgtcc catctgttcg
     1261 ccatcgagac cagctatggc ggccctcggg cgttcgcccg cttcgtcgat gccgcccacc
     1321 agcgcggcat cgcggtgatt ctggacgtgg tgcacaacca cctcggaccg accgatctcg
     1381 acctgtggcg cttcgacggc acgtccagcc gcggctatgg cgggcagtac ttctacaacg
     1441 accggcgggc gctcacgccc tggggggcca cccgtccgaa ctacgaacgt aagcaggtcc
     1501 gcaacttcct gcgcgactcg gcggtgatgt ggctggaaga gttccgggtc gacggcttgc
     1561 gcttcgacgg caccaacttc atccggtccc gctggggcaa cacccagcac cgtcccgatc
     1621 gcatcgccgg cggccaccgc tacctggccg cgacaaccgt cgacatccgc gcccggcagc
     1681 cctggaagct gctgatcgcc gaagacatgc aacgcgaccc ggtggtcacc gcgccggccg
     1741 agcagggcgg gctgggtttc cactcccagt gggacgccga cttcgtgcac cccgtccggc
     1801 acgccctgga gacaccctgg gacgccgatc gcaacacgac ggcggtcgcc cgcgcggtca
     1861 ccggtcacta cctacgggcg gcccacgaac gggtgatctt caccgaatcg cacgacgagg
     1921 tcgccaacgg tcgatcacga ctgccggaga cgatcaaccc gggtgccgcc gactcctggc
     1981 ccgcccgcaa gcgtgcggct ctcggcctcg cgctggtact cacctccgcc ggcatgccgc
     2041 tgctctttca aggccaggag ttctgcgaag accgctggtt cgacgaccgc acacccttgt
     2101 cgtggaacaa gacccagcgc cactcggggt ttctgcacct ggccagacaa ctgatccggc
     2161 tgcgccgcaa tctggacggt cacaccgccg gcctgcgcgg cccccacacc gcgctgctgc
     2221 gggtggacga ccagcgcaag gtgctggcct atcaccgctg gtggcagggc ggccccggcg
     2281 acgacgtgat cgtcgcggtc aacctgtcca ccgaggtgat ccacggctac cgggtcggcc
     2341 tgccccgggc aggccggtgg cagttgcgct gcaacagcga cgcccccatt ttcggtgatg
     2401 acttcggcgc ggcccacgcc agcgacctga acgccgactg cgaaccgtgg gacggccggc
     2461 cgttctcggc cgaggtcacg atcggcccct acgccgcgct ggtgtactcc caggacgaag
     2521 gaccctgact gcggggtcga tgccactgcg tgccgctcac gaccggacga accggcccag
     2581 cccttcgtga ttccaggcgc cgatggtgag cgcggcggcg cgtccgcccg aggtcgtgaa
     2641 ggtggcgccc gacaggcccg tctcgtcctc gccgaccgtc ttgaagaaga actcgtcgcc
     2701 gctgtagtgc tgaaggtcga actgcatctt ggcggggccg gcggtgacgt gcagcgtgcc
     2761 gctgcgatcg ctgaccgcca acgaaccgta gaagctgctg ctgtagcggc caacgtaggc
     2821 ggcgttcgac ctgccccggg tcgggttcgt ggccgcgacc gagtatttct tgttgtcctc
     2881 ggtctcctca ggcatcacga aggggccgat cttcgccacc cagtccgcct cggtcgacct
     2941 gccgtagcgg acgtggtcgt agaactcgac gcccaccgcc tcgggcaggc cgatcggcgg
     3001 cccgttggtg atcaccgcga tacccagctg gtgggtcggg tacacggtga ccgtggtggc
     3061 cgcgcccagc ccgaacccac cggagtggtt gacccgcagc tcgccggtcg gctcgtagct
     3121 gacgatccag cccatgccgt agaaaccggc cgactttccg atcggcggca cgcccggccg
     3181 gaccaccgca ggcagccaga tccgttgcag ctggtcctcg tcgatcaccg gtttgccgtt
     3241 ggccaacagc atggtcaacc aggtcgccag gtcgcggatc gagctgctgg acgagccggc
     3301 cggcgcctgc gcgtccgtgt cgaccttcag gttcggcacc caggtgccgt ccacctgcac
     3361 gtgcatggcc gcccggttgg tgcgcgactg caggtcggcg aaccggtagc tggtgtcctt
     3421 catgcccagc ggaccgaaca gcacctgctc tgccaactcc tcccacttca ggccggacga
     3481 gaccgcgatc gcctcggcgc cggcggtgat gccgtagttg gtgtaggcgt agttgtcgcg
     3541 gaaccgcttc agcgggtaga agcgatgctt ggccaggatc gtggcccggt cgtagccgag
     3601 gccttcgagc aggttgccgg agtgatccgg caggccgctg cggtgcgcga acatgtcggc
     3661 gacggtgacg tgactgccgg tccaagcgtc cgacagggtg aagtcgggta gcgccttctg
     3721 caccgggtcg tcccaaccca gcgtgggcag cttcttggtc atcgcggcgg cgaccacggt
     3781 ggcgctgatc ggcttggaga ccgaggcgag ctggaacacc gtgtccgcgt cgggcttgtc
     3841 gggcttgccg acctcgcgca ggccgaaccc ctccaggtag cgcacttggc cctcgtggac
     3901 gatggcgatc gcgagcccgg gcaccccgta ctgctgcagg tagcgaacga tgatgccggg
     3961 cagcgcgtcg accccgcgct gcacggcggc cgcatcgatg acgtccgcgc tcgaaggcac
     4021 cccggaggcc tggccggacg ggctgacggt ggcggccggg gcggacggtc cggtgcaggc
     4081 gctgaggccg gcggctgccg cagcggccgc accccccaat ccgacgcctc tgagcagggt
     4141 tcggcggccg actgccgcgg gggtggcgat ctgggacggt gcaggcatgg tgacagcgtg
     4201 cctgccgagg gcccgtcgcg cccatagacc cagcccgcca gccacccagg acctaggtcg
     4261 tgtccgccgg tcgggcgtgg cacgaccacg tcgtcggtgc tcaacccagg agcttgcgac
     4321 ccaccaccgg cgcgtaaccg agcagttgac ccagcacgtt gggatgaaac gccgcgaccg
     4381 ggccggccgg cccggtgatc cacgggaccc gagcgcacca ggcgtggctg ttgaacgcac
     4441 tttcggcgtt cgcgaaccgc gcaccgacct gccgggccgc gtcggcgagt tggtcgttga
     4501 gttcgtcgat ggtgcggttg atcagcttct gttcggtggc gctgaactcg accagggggc
     4561 tgcaatcggt gccgctgaac agtcgcggat accccaccac gaccatggtc gcctccggcg
     4621 cccggtcccg caccgcggcg aacagggcgg tcgcgttggc gccgaacccg ccctcgaggt
     4681 aggcgcggcc ctgagccacc gcggctgcgc acgcatcgtc gctcgccggc tgggcacaca
     4741 gtgcgagaac cggcgcgaac ccgatgtcgt tgccgcccgc ctgcacggtg acgaagtcgg
     4801 cggagtcggt gacctgcccg agttgggtgc gggccacgtc ggccatggtg gcgccgctgc
     4861 aggcggccag cgtgaggtcg aggcgggcga ccttggcgat gacgctcggg tagccgaagg
     4921 acgatcgtcc gcacgagtcc ttcggcgctg cggcgcccag gccggcggcg taggagtcgc
     4981 cgagggccac atagccgcgc ggtgcgcggt gccggggtac ggcggcgtcc gcgggagcaa
     5041 tggcgttggc gaatagggca acggcggcga tcagggtggc gaccacccgg cggagtcggg
     5101 acacgacgaa cctatctctc ccaaggcgaa gagagcatgt catcgccgtg ggcaccgcag
     5161 aaggtggcag atgtacccat gggttccgac cgtcggccgg ttgacaaggc agggtcagga
     5221 gccgaagtgt tccctcaaca gccgccgcca cttgttggac ggcaggtgcg ggtgcatcgc
     5281 ggccagcgcg gtcgcgtact tcgacacgct gctctcacga tgaccgagcg accacaacag
     5341 gcgatccatg cccgtggcac gtccggtgga cttggtctcg atgatcgaca ggtccggaag
     5401 gctcagccag gcgccggtgc gcacgtccca ccaggtcagg cccgagtcga tcgtgacccg
     5461 ggtcgcggtc ggctcgtgaa acagggtgac gcggcggtag tcgacgcgga gcgaggccga
     5521 caggtcctcc accggggccg aacggacgcc cgcgcggcgc agtgccgcgc ggacgaacgc
     5581 cgcaccctcg tcgctgagcc gtccgtcgac caggtgggtg cccggcagcc gatccttgac
     5641 ggtgtgccgg tgctgccgcg tcttcacctc cagaaaactg gcgccgttgg actcatacgt
     5701 gcgggtgcgc accttgaacc ggcggcgccg ctggcgggcg gccagtagaa aggtgtccag
     5761 cccgggggtg tcgaggtaga ccgagctgta ccccaggttc gggacaccgt cgatggccag
     5821 cacccgggtc gtcgccggat cgaccagttc aacggccatg ccggcgtccg ccgcagtcag
     5881 ggcgtatttg cggtccaccc gggtgagcag gctgccgcgt tcgacgagct cgtccaggcc
     5941 gatggtgtcc agcgcggggt aggtggtgat catgctgctc ctgtcggttt gctgttcacc
     6001 agaagttcac caacagtggc tgtgccgcac ctgtgtggtc gtcggcaacc gaccgtgagg
     6061 ttccgtcagg gagttcgacg acggtgaacg ccgtccgccg gacacggctg gtcgatgtcg
     6121 gtgaccttgg acgacgtgcg cgggctgggg gtcggcgtgc agacgtccac cacgtcacag
     6181 cgggcaaggg cttcgtcgac gccgtcgcgt ccgaccaggt ggcctggtca ccttcgccgt
     6241 gggtgtggag gttgaccatg ccgggcaccg gtgcggtgag ccggatcagg gcctgctgct
     6301 cgccgatgat ctcgacctcg ccggtggcgc ggtccaggtc gatcgcgtcg tcctcgtcgc
     6361 ggatccgcag tgcggccgcg cccggtcggc taggttgcgt gacgtgccgg tcgaccgctc
     6421 cgccattcgg gtgaaggcga tgctgatcgc gcccaacggg ggtcgcaccg cccacgcggt
     6481 cagcgtgcat gcccccacgc tcgagaaccc ccgcggcttc catcgcctga tcggcggcag
     6541 tgtggagctg ggcgagcgac acctcgacac gattcgtcgt gagatcaccg aagagttggg
     6601 ggccgccgtc cgcgacctcg tgctgctcga cacggtggag aacatcgtca ccatcgacgg
     6661 cgtgatcgga cacgagatcg tctttctgtg caccggtcag ctggaaccgc agccgccggc
     6721 ggccggggca acgctcaccg agatcgacgg atcggtggtg ccggtggtgt ggcgctcgat
     6781 cagcggcgag ggcgaaacag tgccgctcta tccgagcgca gccgagtggt gggtgaccca
     6841 cgtcgcgacc ggcttaccct gaggccatgg acgacagtcc caccgcgcgc gccctgcgca
     6901 cgctcgaggc gatccacaac cggccgggca tcagcgcccc cgaactggcc gaccggctgg
     6961 gtgtgaccga gcgggccgtg cgccgctacg tcggtacgct gcgcgacgcc ggcgtgccgg
     7021 tcaccggcac cgcgggacgc tacggcggct accagatcgg ccgcggcgtc cggctgccac
     7081 cggtggcgtt caccggcacc gaggcgctcg cgctggtgat ggcggtggtc gaatcacggt
     7141 caggtgcgcc ggccggcaca ccggctggcg agggtgtcgg caagctgctg cgcgcactgc
     7201 ccgaggccgt ggcgcgctcg gcgcgccagt tgtggcagca cacggccgcg gcaccgtccg
     7261 acttccctcg acccgatccg ggtatcaccg gcggcctgat cgaggccgtc gcagccggac
     7321 ggcggacgcg gctggggtac cggagcgcgt ccggccacga atggacgggc gaggtcgatc
     7381 cctgggcggt cgtggtgcgg cgcggcctgt ggtacctact gtgcgccaac gccaccgcag
     7441 aggtgcgcac ctatcgggtc gaccgggtga ccgccgtgga ggtgctgggc acacccgccg
     7501 acgtacccgc cgacctggac gccgccgccg ccctcgaacg cggtctcagc cagggctggc
     7561 gccaccccac ccgggtgcgg ttcgccgatc cggtcgagcg ggtccgcgac tggctgcccg
     7621 ggccgatggg cgacttggtt ccggacggtg acgggtgcct gctgaccggc accaccaaca
     7681 acccggacat gtatgcgggg gagtggctcg cccgggtcga gctgccgttc gtggtggagg
     7741 gcggcgacga actcatcgcc gccatgcacc ggctggtcgc caggctgcac gccgcactac
     7801 cggcgcaggg gtgaatcagc gctgcggatc agccccgccg acggacgaac tcgcgccgcg
     7861 cgtactggcc cagcgcgtag gtgaagtagc tgtcggcggc ggctccgaca ccgcccccga
     7921 ccagcgggat gcgcttggcc acctgcacca ccacatgctt gccggccatc ttggtgccca
     7981 ggtcgccgaa cacccgctcg ctgacgtggc gatccagcgc ggggtcgaat accggtgcgg
     8041 tggccaccac catcggggtg gcgggcaact tgccggaaga gacctgcttg cgcacctcgt
     8101 cctcgcccag catggccagg gtgagggcga cgcgcatgcg ccgatcgtcc acgtcgtagc
     8161 cgcgtaggtg agcgatcgcc gcgatcatgc gcatctgcac gacggccagg ccggccagat
     8221 tggccggcag cgccaccggc accgtgatca gcccgccgag gttggtcaga aagccctgcg
     8281 caccggcgag cccgacgtgc tgccggatca ccgagtcgac cgcggcctcg tgctcgccgc
     8341 gtgactgcaa cgacttcgcc gctgcgctct tggcccccgg aatgctgccc cggccctcga
     8401 tcgcgaactc gaggatcgcg cgcagcgccc ctccggccac ctcgggggcg cgctggacga
     8461 tcgtcttgct gggttcgtcg ctggacatac gggtgcctct cgttggctgc gccgtccaat
     8521 ctaccgagca ggcccgcggc caccatcggg ggcgaccccg gcgcccaccc cgatccgcgg
     8581 gcccgtggtg gcacaatcgt gcaatgagtc gtccaggcat cttcgggccg tcgcgggact
     8641 ggcccgcgca agacctgatc ggcctctcgg acgagatcga ccccgacctg gtggtgctgg
     8701 cgtaccgctg tggggtcttt ccgatgccgc tgcacaactc gtggtttccg gcccggatgg
     8761 gctggtggtc gccgatgcgg cgcggggtgc tgctgccgga cgggctgcgc gtcacccgct
     8821 ccctacgcaa gtcggcgcac cgctacacca ccaccgtcga cgccgcgttc gaggcggtgc
     8881 tggcccgctg cgccgacccg ggccgtccgc acggctggat cgacgacgac atcgtcgccg
     8941 tcttcaccga cctgcaccgg cgcggggtg
//
LOCUS       DAOSUG010000051         8966 bp    DNA     linear   ENV 10-JUN-2024
DEFINITION  MAG TPA_asm: Micropruina sp. isolate AMR_MDS_2195
            NODE_4135_length_8966_cov_4.526652, whole genome shotgun sequence.
ACCESSION   DAOSUG010000051 DAOSUG010000000
VERSION     DAOSUG010000051.1
DBLINK      BioProject: PRJNA925477
            BioSample: SAMN32864262
            Sequence Read Archive: SRR4236673
KEYWORDS    WGS; ENV; Metagenome Assembled Genome; MAG; Third Party Data; TPA;
            TPA:assembly.
SOURCE      Micropruina sp. (wastewater metagenome)
  ORGANISM  Micropruina sp.
            Bacteria; Bacillati; Actinomycetota; Actinomycetes;
            Propionibacteriales; Nocardioidaceae; Micropruina.
REFERENCE   1  (bases 1 to 8966)
  AUTHORS   Abdulkadir,N., Saraiva,J.P., Zhang,J., Stolte,S., Gillor,O.,
            Harms,H. and Rocha,U.
  TITLE     Genome-centric analyses of 165 metagenomes show that mobile genetic
            elements are crucial for the transmission of antimicrobial
            resistance genes to pathogens in activated sludge and wastewater
  JOURNAL   Microbiol Spectr 12 (3), e0291823 (2024)
   PUBMED   38289113
REFERENCE   2  (bases 1 to 8966)
  AUTHORS   Abdulkadir,N.
  TITLE     Direct Submission
  JOURNAL   Submitted (24-MAR-2023) Environmental Microbiology, Helmholtz
            Center for Environmental Research -UFZ, Permoserstr 15, Leipzig
            04318, Germany
COMMENT     The annotation was added by the NCBI Prokaryotic Genome Annotation
            Pipeline (PGAP). Information about PGAP can be found here:
            https://www.ncbi.nlm.nih.gov/genome/annotation_prok/
            
            ##Genome-Assembly-Data-START##
            Assembly Method        :: SPAdes v. 3.11.1
            Genome Representation  :: Full
            Expected Final Version :: Yes
            Genome Coverage        :: Not Applicable
            Sequencing Technology  :: Illumina MiSeq
            ##Genome-Assembly-Data-END##
            
            ##Genome-Annotation-Data-START##
            Annotation Provider               :: NCBI
            Annotation Date                   :: 05/04/2023 04:57:10
            Annotation Pipeline               :: NCBI Prokaryotic Genome
                                                 Annotation Pipeline (PGAP)
            Annotation Method                 :: Best-placed reference protein
                                                 set; GeneMarkS-2+
            Annotation Software revision      :: 6.5
            Features Annotated                :: Gene; CDS; rRNA; tRNA; ncRNA
            Genes (total)                     :: 2,776
            CDSs (total)                      :: 2,750
            Genes (coding)                    :: 2,714
            CDSs (with protein)               :: 2,714
            Genes (RNA)                       :: 26
            tRNAs                             :: 24
            ncRNAs                            :: 2
            Pseudo Genes (total)              :: 36
            CDSs (without protein)            :: 36
            Pseudo Genes (ambiguous residues) :: 0 of 36
            Pseudo Genes (frameshifted)       :: 2 of 36
            Pseudo Genes (incomplete)         :: 32 of 36
            Pseudo Genes (internal stop)      :: 2 of 36
            ##Genome-Annotation-Data-END##
FEATURES             Location/Qualifiers
     source          1..8966
                     /organism="Micropruina sp."
                     /mol_type="genomic DNA"
                     /submitter_seqid="NODE_4135_length_8966_cov_4.526652"
                     /isolate="AMR_MDS_2195"
                     /isolation_source="Activated sludge of municipal
                     wastewater treatment plants"
                     /db_xref="taxon:2737536"
                     /environmental_sample
                     /geo_loc_name="Canada"
                     /lat_lon="49.25 N 123.1 W"
                     /collection_date="2015-06-16"
                     /metagenome_source="wastewater metagenome"
                     /note="metagenomic"
     gene            <1..426
                     /locus_tag="PLF56_02850"
     CDS             <1..426
                     /locus_tag="PLF56_02850"
                     /EC_number="3.2.2.20"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_012923070.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_function: GO:0008725 - DNA-3-methyladenine glycosylase
                     activity [Evidence IEA];
                     GO_process: GO:0006284 - base-excision repair [Evidence
                     IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="DNA-3-methyladenine glycosylase I"
                     /protein_id="HOQ52533.1"
                     /translation="GLAWITILRKRPAFRAAFADFDPVVVAGFDDGDVQRLMGEAGIV
                     RNRAKIEATIANAQALTALHQRGERLAEVFEAHRPAARPVRPTSFADVPATTAESVAL
                     AKQLKKLGFRFVGPTTAYAAMQALGYVDDHLAGCLTRRH"
     gene            545..1444
                     /locus_tag="PLF56_02855"
     CDS             545..1444
                     /locus_tag="PLF56_02855"
                     /inference="COORDINATES: protein motif:HMM:NF025616.2"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="DUF4349 domain-containing protein"
                     /protein_id="HOQ52534.1"
                     /translation="MHRTPRLLALLALILALVGCSVGSGSQGQVVAQGAAAPAQDSGA
                     LPSGPQATAVPRSPTEPGQGPATERQVTRTGALTIRTGDVQQAAARLREIAQANQGIV
                     VDEQLHVGDSSGTTRVVLSVPSASLTRVMDEAATVGEVVDRSVTATDVTDRVVDVDAR
                     IRTLRASIERIRALMQRTGSIKEIASVEGELTDRQGELESLLATQAALKNQVERAPIT
                     VTLVRPDQLAIPANPLVVGLIQGWEALQKSISVLLTVVGGVLPFALVGALIAWPLVHR
                     RRTRGRRGDENVSPPPDRHASQD"
     gene            complement(1407..3152)
                     /locus_tag="PLF56_02860"
     CDS             complement(1407..3152)
                     /locus_tag="PLF56_02860"
                     /inference="COORDINATES: protein motif:HMM:NF013188.2"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_component: GO:0005886 - plasma membrane [Evidence IEA];
                     GO_component: GO:0016020 - membrane [Evidence IEA];
                     GO_process: GO:0006814 - sodium ion transport [Evidence
                     IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="sodium:proton antiporter"
                     /protein_id="HOQ52535.1"
                     /translation="MSPEAVVIVITLALLCIAGASGLSNLTRVAAPLLLVVFGAVVGY
                     LPGVPALDIEPDWILIGVLPPLLFSSAVSMPAMNFRRELRSITGLSVTLVLVTSLLLG
                     WFFAVTLHTSFAWGVALGAIVSPTDAVATAIARRVGVPHRVLTMLEGESLLNDATALV
                     LLRSAVAAAAGTVALGSVVLDFLWALGAAVAIGFAIGWLHLWVRARVADPTVSTLISY
                     AAPFVAALPAEAVGASGLVAAVLAGLVTGYGAPRMLSPSARLSDSQNWKTVELLLESA
                     IFLLMGLQLHTLINDETHSEGALATVVWVAAAGFALTVLIRAGYVVTVVKATARRTAR
                     QQDRQQRFDGLQRYIDARGAHPEADPEELRHEILGEQSRPTGWQRWLRRVMRPGKAPR
                     TADEQARERWNANRMERIRTAMRRQRATVDYLVRQPIEAREGGLLAWAGMRGAVSLAA
                     AQTLPAATPGRPVLVLVAFFLAGGSLILQGATLPLVVKRLSVPPTPEEVQADADEHAR
                     LLSFVLDVAEQERARVPDRPLKRLELDVLAAQRAALLDARDDGVFDARMLAHLLASVD
                     AQELSLAMRGDPEEG"
     gene            complement(3383..4576)
                     /locus_tag="PLF56_02865"
     CDS             complement(3383..4576)
                     /locus_tag="PLF56_02865"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_020390617.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_function: GO:0005524 - ATP binding [Evidence IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="ATP-binding protein"
                     /protein_id="HOQ52536.1"
                     /translation="MDPIRNPYAPGAGQRPPELAGRDAQLEAFRFVIERVAKGRPERS
                     IVLVGLRGVGKTVLLNAMRSQAVRAGWGTGKLEARPDQTIRRPLGAALHMAIRELAHP
                     DATGVLSTLKAFVERAAPANAKAYDRWNSGITAPTVIGRADSGDIEIDLVELLSDVAG
                     LAADKGKGVAVFIDEMQDLGADDVSALCAACHELGQQGLPLIVVGAGLPHLPAVLSAS
                     KSYSERLFAYARIDRLDRAAADLALASPAADEGAAFTDDALAEMYRVTGGYPYFVQAY
                     GKVVWDLAPRSPITAVDVAVAAPEAEHELAVGFFGSRLERATAAEREYLQAMAEADPD
                     DAGTVPSARVAEVLGRKPQSLSPARDALIKKGLVYASERGRIAFTVPHFGRFLRGRVD
                     MADTA"
     gene            4908..5168
                     /locus_tag="PLF56_02870"
     CDS             4908..5168
                     /locus_tag="PLF56_02870"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_015070148.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="AbrB/MazE/SpoVT family DNA-binding
                     domain-containing protein"
                     /protein_id="HOQ52537.1"
                     /translation="MSASYTTITAKGQITLPAEARSALGLRVGQKVAVRVEGDHLVID
                     PPHDVGALRERLQAEAQEHGTWGTVPVAGDGWAAHADEEPSN"
     gene            5248..5729
                     /locus_tag="PLF56_02875"
                     /pseudo
     CDS             5248..5729
                     /locus_tag="PLF56_02875"
                     /inference="COORDINATES: protein motif:HMM:NF016362.2"
                     /note="frameshifted; Derived by automated computational
                     analysis using gene prediction method: Protein Homology.
                     GO_function: GO:0003677 - DNA binding [Evidence IEA];
                     GO_function: GO:0004519 - endonuclease activity [Evidence
                     IEA];
                     GO_process: GO:0009307 - DNA restriction-modification
                     system [Evidence IEA]"
                     /pseudo
                     /codon_start=1
                     /transl_table=11
                     /product="restriction endonuclease"
     gene            5838..6338
                     /locus_tag="PLF56_02880"
     CDS             5838..6338
                     /locus_tag="PLF56_02880"
                     /inference="COORDINATES: ab initio
                     prediction:GeneMarkS-2+"
                     /note="Derived by automated computational analysis using
                     gene prediction method: GeneMarkS-2+."
                     /codon_start=1
                     /transl_table=11
                     /product="hypothetical protein"
                     /protein_id="HOQ52538.1"
                     /translation="MSRGRPTRASVYARLEAALDELRTRLGGLPTPEESAFVWRDIWH
                     LEAHHSTAIEGNTLVIREVEVLLEQGRAVGAKPLKEYMEVQGYAEAARWVYAQAHNSE
                     QLSQGELVTLTELRQIHATLMAPVWQVAPHPDANEREAPGHFREHDIHPFDGGMTPPV
                     GRWCRR"
     gene            6335..6853
                     /locus_tag="PLF56_02885"
     CDS             6335..6853
                     /locus_tag="PLF56_02885"
                     /inference="COORDINATES: protein motif:HMM:NF014691.2"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="Fic family protein"
                     /protein_id="HOQ52539.1"
                     /translation="MIRDWLDSANAAGDRLNNAAYGPLPEQLATLHNDFEKIHPFIDG
                     NGRAGRLVLNLLLVRLGYPPVVIMKQQRPVYLSAMQRADDGDPGLLGELLARAMLDNL
                     NRFILPSVARPARLVPLAALVDERFSIAALRQAAQRGRLDAFQGADGVWRSSRRAVDD
                     YARTKGQRRKGE"
     gene            7014..7529
                     /locus_tag="PLF56_02890"
     CDS             7014..7529
                     /locus_tag="PLF56_02890"
                     /inference="COORDINATES: protein motif:HMM:NF020052.2"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_function: GO:0003677 - DNA binding [Evidence IEA];
                     GO_function: GO:0003824 - catalytic activity [Evidence
                     IEA];
                     GO_process: GO:0006304 - DNA modification [Evidence IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="type I restriction-modification enzyme R subunit
                     C-terminal domain-containing protein"
                     /protein_id="HOQ52540.1"
                     /translation="MDVALPYTTPGTDTVRFRSKAAAYLRHHEDHVALQRLRRNKQLT
                     SDDVSSLERMLLGAGGRADDVKLAARQAGGLGLFIRGLVGLDLCAATEAFGHFLIDTR
                     FTVDQIRFVQLIVDELTATGVMEPRRLFESPFTDHAPTGPDQVFDDGDLASIVDILRA
                     VKRHAMPVEVA"
     gene            complement(7808..8635)
                     /locus_tag="PLF56_02895"
     CDS             complement(7808..8635)
                     /locus_tag="PLF56_02895"
                     /EC_number="1.1.-.-"
                     /inference="COORDINATES: protein motif:HMM:NF015411.2"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_function: GO:0050661 - NADP binding [Evidence IEA];
                     GO_function: GO:0051287 - NAD binding [Evidence IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="NAD(P)-dependent oxidoreductase"
                     /protein_id="HOQ52541.1"
                     /translation="MKIAVLGLGNMGLPVAVNLARAGHDVTGANIAAVDAPGVSVTTD
                     ATVALADREVTFCLLPDLPYLEPMLPALIEGGCLDLVVMSTISPTAVRGLAERLQGQV
                     SVMDACMSGGVAKAVDGTMSIMCGADEEMFARVRPLLDVIGGTVELMGDVGAGAVTKA
                     CNQMVVAGTLVALAEATLVAKENGLDAHRVLAVLGAGLAASELLDQKAAKLADEDFSP
                     SGPAKFLVKDLTFATDSAAGRSLPSLAVSLELFSGLTDAGLGDQDNSVVLEYLRRIS"
     gene            complement(8632..>8966)
                     /locus_tag="PLF56_02900"
     CDS             complement(8632..>8966)
                     /locus_tag="PLF56_02900"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_013862281.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_function: GO:0051287 - NAD binding [Evidence IEA]"
                     /codon_start=3
                     /transl_table=11
                     /product="NAD(P)-dependent oxidoreductase"
                     /protein_id="HOQ52542.1"
                     /translation="AMKPSAVLLNMARGPIVDERALIAALRSGGIRAAGLDVFEDEPP
                     TSGIETLPNVTLAPHSIAWTSEMSLGNGNSCVDALLAVARGEAPRFVVNRDVLSTPAF
                     VKRLKEWA"
BASE COUNT         1507 a         3071 c         3028 g         1360 t
ORIGIN      
        1 ggtttggcgt ggatcacgat cctgcgcaag cggccggcct ttcgggctgc gttcgccgac
       61 ttcgatccgg tcgtcgtcgc cggcttcgac gacggcgatg tgcagcgatt gatgggtgag
      121 gcgggcatcg tccgcaatcg ggcgaagatc gaggcgacga tcgccaatgc gcaggcgctg
      181 acggcgctgc accagcgggg tgagcggctg gctgaggtgt tcgaggcgca ccgccccgcg
      241 gcgcgtccgg tgcggccgac ctccttcgcc gacgtgcccg ccaccaccgc cgagtcggtt
      301 gccctggcga agcaactcaa gaagctgggc ttccgcttcg tcggtccgac cacggcgtat
      361 gccgcgatgc aggccctcgg ctacgtggac gatcatctgg ccggctgcct gacccgtagg
      421 cactgaccat cagggcgtaa ccgtttttga gcccccgcac gccccccgag acaccgcagt
      481 caccctgacg cgctgatccc cccgccgctg caacgacgcc gcgcccggcc gcgtgtatgt
      541 aggcatgcac cggacgcccc gactacttgc cctgttggcc ctgatccttg cgctcgtggg
      601 gtgcagcgta ggcagtggca gccagggcca ggtcgtcgcc cagggcgcgg cagccccggc
      661 gcaggattct ggcgcgctgc cgtccggccc gcaggccacc gcggtgccga gatcgcccac
      721 cgagccgggg cagggtccag cgaccgagcg ccaggtgacg cgcaccggcg cactgaccat
      781 ccgcaccggc gacgtgcagc aggcggccgc ccggctccgc gagatcgcac aggcgaacca
      841 gggcatcgtg gtcgacgagc aactccacgt cggcgactcg tccggcacca cgcgggtcgt
      901 gctcagcgtg ccgagcgcct ccttgacccg ggtgatggac gaggcggcga cggtcggcga
      961 ggtcgtcgac cgcagcgtga cggccacgga cgtcaccgat cgagtggtcg atgtggatgc
     1021 acgcatccgc accctgcggg cgtccatcga acggatccgg gcgctgatgc aacgcaccgg
     1081 ctcgatcaaa gagatcgcct cggtcgaggg cgaactgacc gatcgtcagg gcgagctgga
     1141 gtcattgctg gccacccagg cggccctgaa gaaccaggtc gagcgggccc cgatcaccgt
     1201 gacgctggtt cgtcccgatc aactcgcgat acccgccaat ccgctggtgg tcggcctgat
     1261 tcagggctgg gaggcgttgc agaagtcgat ctcggtgctg ctcaccgtgg tgggcggcgt
     1321 gctgccgttc gccctggtcg gagccctcat cgcgtggccc ctggtgcatc gccgccgcac
     1381 cagaggcagg cgtggggacg agaacgtcag ccctcctccg gatcgccacg catcgcaaga
     1441 ctgagctcct gcgcgtccac ggaggccagc aaatgggcca gcatccgggc atcgaacaca
     1501 ccgtcgtcgc gggcgtccag cagggcagcg cgctgcgcgg cgagcacgtc gagttcgagt
     1561 ctcttgagcg gacgatccgg cacccgcgcc cgctcctgct cggccacgtc caggacgaag
     1621 ctcagcaacc gagcgtgttc gtcggcgtcg gcctgcacct cctcgggcgt gggcggcacg
     1681 ctgagccgct tgaccaccag cggcagcgtc gcgccctgga ggatcagcga tccgccggcc
     1741 agaaagaatg cgaccagcac gagcaccgga cgtccgggcg tggccgcggg cagggtctgt
     1801 gcggcggcca gtgacacggc gccgcgcatg ccggcccacg ctagcagacc tccctccctg
     1861 gcctcgatcg gctgacggac caagtagtcg acggtggccc gctggcgccg catggcggtg
     1921 cggatccgct ccatccggtt cgcgttccag cgttcgcgcg cctgttcgtc cgcagttcgg
     1981 ggggccttgc cgggacgcat cacccggcgc aaccagcgtt gccagccggt gggacgcgat
     2041 tgctcgccca ggatctcgtg gcgcaactcc tcgggatccg cctcggggtg tgcgccgcga
     2101 gcatcgatgt agcgctgcag gccgtcgaat ctctgctgcc ggtcctgttg ccgggcagtg
     2161 cgacgggcgg tcgccttcac cacggtcacc acgtacccgg cacggatcag cacggtcaac
     2221 gcgaagccgg cggccgccac ccacacgacc gtggccagtg cgccctcgct gtgcgtctcg
     2281 tcgttgatca gggtgtgcaa ctgcaacccc atcagcaaga agatcgcgct ctcgagcagc
     2341 agttcgacgg tcttccagtt ctgcgagtcc gacagtcgcg cactgggtga cagcatgcgg
     2401 ggggcgccgt agccggtgac cagtccggcc agcacggccg ccaccaggcc ggacgccccg
     2461 acggcctcgg ccggcagcgc cgcaacgaac ggcgcggcgt acgagatcag tgtcgagacg
     2521 gtggggtcgg ccacccgggc gcgcacccac aggtgcaacc agccgatcgc gaaaccgatc
     2581 gcgaccgcgg ccccgagggc ccacagaaag tcgagcacga ccgaccccag ggccaccgtt
     2641 cccgcggctg cggcgaccgc cgaacgcagc agcaccaggg ccgtcgcatc attgagcagg
     2701 ctctcgccct cgagcatggt gagcacccgg tgcggtaccc ccacccggcg ggcgatggcg
     2761 gtggcgacgg cgtccgttgg gctgacgatg gcgcccagcg cgacacccca ggcgaacgag
     2821 gtgtgcaggg tgaccgcgaa gaaccagccc agcagcaggc tggtgaccag caccagggtc
     2881 accgacaggc cggtgatcga gcgcagttcg cgccgaaagt tcatcgcggg catcgacacc
     2941 gccgacgaga acagcagcgg cggcagcacg ccgatcagaa tccagtctgg ttcaatgtcg
     3001 agcgccggaa cacccggcag gtagccgacc accgcgccga acacgaccag cagcaacggc
     3061 gccgccaccc tggtcaggtt gctcagcccg gacgcccccg caatgcacag cagcgccagg
     3121 gtgatcacaa tgacgacggc ctcggggctc acgcgctcag catgccgcat gcacctgcga
     3181 gcgcccatgg tcggacgagc cgtgcgacca acgacacgcc gggtcctctc gctgaggatg
     3241 gcaacgcagc gagcgggcgt accgggtgcg cagcacggtt ggggctgtgg cgggtgatct
     3301 gacgggcgcg acgcgccctg gtcgcatggg actcgacaag ctcgaccggc gatggatcgg
     3361 ccagtgctgg agctgagcgg agctaagcgg tgtcggccat gtcgacccgg ccgcgcagga
     3421 accgtccgaa gtgcggcacg gtgaacgcga tccggccccg ctcgctggcg tacaccagtc
     3481 ctttcttgat cagcgcgtcc cgggccggcg acaacgattg gggcttgcga cccagcacct
     3541 cggccacccg tgcggacggc accgtgcccg cgtcgtccgg gtcggcctca gccatcgcct
     3601 gcaggtactc gcgctcggcc gcggtggccc gttcgagccg tgagccgaag aagccgaccg
     3661 ccagctcgtg ctcggcctcg ggcgccgcca ccgcgacatc gacggcggtg atcgggctgc
     3721 ggggcgcgag atcccagacc accttgccgt aggcctgcac gaagtagggg tagccgccgg
     3781 tgacccggta catctcggcg agcgcgtcgt cggtgaaggc ggcaccttcg tcggcggcgg
     3841 gggaggccaa cgccaggtcg gcggccgcgc ggtcgagacg gtcgatgcgc gcgtaggcga
     3901 acagccgctc cgaatacgac ttggacgcac tgagcaccgc cggcaggtgg ggcaggccgg
     3961 cgccgacgac gatcagcggc aggccctgct gcccgagctc gtggcaggcc gcgcacaacg
     4021 ccgacacgtc gtcggcgccc aggtcttgca tctcgtcgat gaagacagcc acgcctttgc
     4081 ccttgtcggc ggccagtccg gcgacgtcgc tgagcagttc gaccaggtcg atctcgatgt
     4141 cgccggagtc ggcacgtccg atcacggtgg gggcggtgat gcccgagttc cagcggtcgt
     4201 aagccttggc gttggccggg gccgcccgtt cgacgaaggc cttgagcgtg gacagcacgc
     4261 cggtggcgtc ggggtgggcg agctcgcgga tcgccatgtg cagtgccgcc ccgagcggac
     4321 ggcgaatggt ctgatcgggt cgtgcttcga gcttgccggt gccccagccc gcgcgcacgg
     4381 cctgcgaccg catggcgttg agcagcacgg tcttgccgac cccccgcaac ccgaccagca
     4441 cgatcgagcg ttccggacgc cccttcgcga cccgttcgat cacgaaccgg aaagcctcca
     4501 actgcgcgtc ccggccggcg agttcaggcg gacgctgccc ggcgccgggt gcgtaagggt
     4561 ttctgatcgg atccatgatc ggactgtatg gggcaatcta gcgtccgcca cagattaccg
     4621 aagagtgtcg cagagacggt tacggagaac ctccagagtc tggccgctcg ctgctgagcg
     4681 gggccacgat gaggtcgcct gcagaagagc cggagaagat ttcgcctcgt gaagatatac
     4741 cgatattacc gccagattcc agatggaacg attcaaagcg tcatttgaca aatctggcgg
     4801 taatatcggt atatcaatgg gtgatgaaaa atggagcatg ccaggtgtgg aacctggacg
     4861 gttcatgagc catgcggatc tcataaaacc tgagctactt ttccaacatg agcgcctcgt
     4921 acaccacgat cacggcgaag ggtcagatca ctttgccggc cgaagcgcgc agcgcgctcg
     4981 ggttgcgcgt ggggcagaag gtcgcggtgc gggtcgaagg cgaccacctc gtgatcgacc
     5041 ctccccacga cgtcggcgca ctgcgcgagc gtctgcaggc cgaggctcag gagcacggca
     5101 cgtggggcac cgtgcccgtg gccggtgacg gctgggcggc gcacgccgac gaggaaccat
     5161 cgaactagct gtcatgccga gcgtcgacac gaatgtgctc tcgctttggc tgctggcgac
     5221 cactttgccg cgggcgttgg tgcggacatg cccgaccaag ccgagcgcgt ggaccacttg
     5281 cttacctccg gaaaacaact caccgttgat gacgccgcgc tgatcgaggt ggtgctcgtg
     5341 ctggaacggg ttggctacgg aggcgccgac cggcaggcga aggtgacgca gcagtccaat
     5401 gacggcggca tcgacggcat catcgaccag gacaccctcg ggctcaaccg ggtgtacatc
     5461 cagccaaacg ttacgcaccc gacagctcgg tgcagcgtcc cgaactgcag gctttcgtcg
     5521 gggcactcag cggcaaggcc gatggcggcg tgttcatcac caccggaaaa ttcagtcagg
     5581 gcgctcggga ctacgccgcc aatgtgccca cccgagtcat cctgatcgac gggactcggc
     5641 tgagttcgct gatgatcagg ttgggcgtcg gcgttcaggt cagacagacc gtccacctgg
     5701 tagagatcga cgaggacttc ttcgagtagg ccggactcga agttgattag atcatttggg
     5761 tctcagcagg ttgtatcaac gccggtagtt gatacgatca tttaggtagt cgctacctat
     5821 atcaacttgg agcaacgatg tcccgagggc gcccgacccg agccagcgtt tacgcgcggc
     5881 tcgaggcggc actcgatgag ctccgcacgc gcctcggtgg cctgcccacg ccggaagagt
     5941 cggccttcgt gtggcgcgac atctggcacc tggaggcgca ccactccacg gcgattgagg
     6001 gcaacactct ggtgatccgc gaggtcgagg tgctgctcga acagggcagg gccgtgggcg
     6061 ccaagccgct caaggagtac atggaggtcc agggctacgc cgaggcggcc cggtgggtct
     6121 atgcccaagc ccacaacagc gagcagttga gtcagggcga actcgtcacg ctgacggagt
     6181 tgcgccagat tcacgcgacg ctgatggctc cggtgtggca ggtcgctcca caccccgacg
     6241 cgaatgagcg ggaagcgccc gggcactttc gcgagcacga tatccatccg ttcgacggtg
     6301 gcatgacgcc cccagttggg cgctggtgcc gccgatgatt cgcgactggc tcgactccgc
     6361 gaacgctgcc ggcgaccggt tgaacaatgc ggcgtacggc ccattgcccg agcagttggc
     6421 gacgctgcac aacgacttcg agaagatcca tccgttcatc gacggcaatg gccgggccgg
     6481 ccggctagtg ctgaacttgt tgttggtccg gctgggctac ccgccggtgg tgatcatgaa
     6541 gcagcagcgt ccggtctatc tgtcggccat gcagcgggcc gacgacggtg atcccggact
     6601 gctcggcgaa ctgctggcac gagccatgct cgacaacctc aaccgcttca tcctgcccag
     6661 cgtcgcccga cctgcgaggc tggtgccctt ggcagcgctg gtcgatgaac ggttcagcat
     6721 cgcggcgctg cgccaggccg cgcaaagggg acgtctggac gcattccagg gcgccgacgg
     6781 ggtctggcgc agctcgcgcc gagccgtcga cgactacgcc cgcaccaagg gacaacggcg
     6841 gaagggggag tgatgagcaa cttcacgttc ctgagcgcca cgctgcccgg cgcctacgcc
     6901 gattgcgccc gggcggagtc gtacgtcacg tccgacccgc gggcggcctg cgtgtacgcg
     6961 cggcgggcag tcgagttgtt ggtgaaccat ctgtacgcgc cctcggcgaa gcggtggacg
     7021 ttgcgttgcc ctacacgaca ccaggaaccg acaccgttcg cttccgctcc aaggcggccg
     7081 cctacctgcg gcaccacgag gatcacgtgg ccctccagcg gttgcgtcgc aacaagcaac
     7141 tgacgtcgga cgacgtgtcg tcccttgaac gaatgctgct cggggccggc ggacgagcgg
     7201 acgatgtcaa gctcgctgcc cggcaagccg gaggactggg gctcttcatt cgtggcctgg
     7261 tcggcctcga tctgtgcgcc gctaccgagg cgttcgggca ctttctgatc gacacccggt
     7321 tcaccgtcga ccagatcagg ttcgtccagc tgatcgtcga cgaactcacc gccaccggag
     7381 tgatggaacc gcgcaggctg ttcgagtcac cattcaccga tcacgcgccg accggccctg
     7441 atcaggtctt cgatgacggt gacctggcga gcatcgtcga cattctgcgc gcggtgaaac
     7501 ggcacgcgat gcccgtcgag gtcgcctgat cactatccgg cggggatccc ggctcgactg
     7561 atccggggga gatcccgggg caagcccggg atgacgtggc ccccgtcgtc gtggctccat
     7621 cccgtcgtcg cggctcgact ccgtcatccc ggcgaacgcc gggatctcga ctgatcggcg
     7681 caggtactca gcaccatcaa ctgatccggc ggagatcccg gggcaagccc gggatgacgt
     7741 gcccccgtcg ccgtggctcc atcccgtcgt cgcggccgac tccgtcatcc cggcgaacgc
     7801 cgggatctca actgatccgg cgcaggtact ccagcaccac gctgttgtcc tgatcgccca
     7861 gtccggcgtc ggtgaggccg ctgaacaact ccagcgacac cgcgagggag ggcagcgaac
     7921 gtccggcggc gctgtcggtg gcgaaggtaa ggtctttgac caggaacttc gccggaccgc
     7981 tgggcgagaa gtcctcgtcg gccagcttcg cagctttctg atccagcaat tccgacgccg
     8041 ccaatccggc gccgaggacg gccagcaccc gatgggcgtc caggccgttc tccttggcca
     8101 ccagggtcgc ctcggccagc gcgaccaggg tgccggcgac caccatctgg ttgcaggcct
     8161 tcgtgaccgc cccggcgccg acgtcaccca tcagctcaac ggtgccgccg atcacgtcca
     8221 gcagcggacg cacccgggcg aacatctcct cgtcggcgcc gcacatgatc gacatggtgc
     8281 cgtcaacggc cttcgcgacg ccgcctgaca tgcaggcgtc catcacgctg acctggccct
     8341 gcaaccgctc ggccagccct cggacggcgg tgggcgagat cgtgctcatc accaccagat
     8401 cgagacagcc gccctcgatc agcgcgggca gcatcggctc cagatagggc aggtcgggca
     8461 gcaggcagaa ggtcacctcg cgatcggcca gcgcaacggt cgcgtccgtg gtgacggaga
     8521 cgccgggcgc gtccaccgcg gcaatgttcg cgccggtcac gtcgtgaccg gctctggcca
     8581 ggttgaccgc caccggcagt cccatgttgc ccagccccag gactgcgatc ttcatgccca
     8641 ttccttcaat ctcttcacga acgcgggtgt gctcagcaca tcacgattca ccacgaagcg
     8701 tggcgcctcg ccccgggcga ccgccagtag cgcatccacg caactgtttc cgttgcccag
     8761 gctcatctcg gacgtccacg cgatcgagtg cggcgccagc gtcacgttcg gcagcgtctc
     8821 gatgccggac gtcggcggct cgtcctcgaa gacatccaac ccggcggcgc ggataccgcc
     8881 ggaacgcagc gcggcgatca gggcccgctc gtcgacgatc ggaccccggg ccatgttgag
     8941 cagcaccgcg gacggcttca tcgcag
//
LOCUS       DAOSUG010000052         8902 bp    DNA     linear   ENV 10-JUN-2024
DEFINITION  MAG TPA_asm: Micropruina sp. isolate AMR_MDS_2195
            NODE_4163_length_8902_cov_3.895106, whole genome shotgun sequence.
ACCESSION   DAOSUG010000052 DAOSUG010000000
VERSION     DAOSUG010000052.1
DBLINK      BioProject: PRJNA925477
            BioSample: SAMN32864262
            Sequence Read Archive: SRR4236673
KEYWORDS    WGS; ENV; Metagenome Assembled Genome; MAG; Third Party Data; TPA;
            TPA:assembly.
SOURCE      Micropruina sp. (wastewater metagenome)
  ORGANISM  Micropruina sp.
            Bacteria; Bacillati; Actinomycetota; Actinomycetes;
            Propionibacteriales; Nocardioidaceae; Micropruina.
REFERENCE   1  (bases 1 to 8902)
  AUTHORS   Abdulkadir,N., Saraiva,J.P., Zhang,J., Stolte,S., Gillor,O.,
            Harms,H. and Rocha,U.
  TITLE     Genome-centric analyses of 165 metagenomes show that mobile genetic
            elements are crucial for the transmission of antimicrobial
            resistance genes to pathogens in activated sludge and wastewater
  JOURNAL   Microbiol Spectr 12 (3), e0291823 (2024)
   PUBMED   38289113
REFERENCE   2  (bases 1 to 8902)
  AUTHORS   Abdulkadir,N.
  TITLE     Direct Submission
  JOURNAL   Submitted (24-MAR-2023) Environmental Microbiology, Helmholtz
            Center for Environmental Research -UFZ, Permoserstr 15, Leipzig
            04318, Germany
COMMENT     The annotation was added by the NCBI Prokaryotic Genome Annotation
            Pipeline (PGAP). Information about PGAP can be found here:
            https://www.ncbi.nlm.nih.gov/genome/annotation_prok/
            
            ##Genome-Assembly-Data-START##
            Assembly Method        :: SPAdes v. 3.11.1
            Genome Representation  :: Full
            Expected Final Version :: Yes
            Genome Coverage        :: Not Applicable
            Sequencing Technology  :: Illumina MiSeq
            ##Genome-Assembly-Data-END##
            
            ##Genome-Annotation-Data-START##
            Annotation Provider               :: NCBI
            Annotation Date                   :: 05/04/2023 04:57:10
            Annotation Pipeline               :: NCBI Prokaryotic Genome
                                                 Annotation Pipeline (PGAP)
            Annotation Method                 :: Best-placed reference protein
                                                 set; GeneMarkS-2+
            Annotation Software revision      :: 6.5
            Features Annotated                :: Gene; CDS; rRNA; tRNA; ncRNA
            Genes (total)                     :: 2,776
            CDSs (total)                      :: 2,750
            Genes (coding)                    :: 2,714
            CDSs (with protein)               :: 2,714
            Genes (RNA)                       :: 26
            tRNAs                             :: 24
            ncRNAs                            :: 2
            Pseudo Genes (total)              :: 36
            CDSs (without protein)            :: 36
            Pseudo Genes (ambiguous residues) :: 0 of 36
            Pseudo Genes (frameshifted)       :: 2 of 36
            Pseudo Genes (incomplete)         :: 32 of 36
            Pseudo Genes (internal stop)      :: 2 of 36
            ##Genome-Annotation-Data-END##
FEATURES             Location/Qualifiers
     source          1..8902
                     /organism="Micropruina sp."
                     /mol_type="genomic DNA"
                     /submitter_seqid="NODE_4163_length_8902_cov_3.895106"
                     /isolate="AMR_MDS_2195"
                     /isolation_source="Activated sludge of municipal
                     wastewater treatment plants"
                     /db_xref="taxon:2737536"
                     /environmental_sample
                     /geo_loc_name="Canada"
                     /lat_lon="49.25 N 123.1 W"
                     /collection_date="2015-06-16"
                     /metagenome_source="wastewater metagenome"
                     /note="metagenomic"
     assembly_gap    76..85
                     /estimated_length=10
                     /gap_type="within scaffold"
                     /linkage_evidence="paired-ends"
     gene            complement(<86..626)
                     /locus_tag="PLF56_02905"
     CDS             complement(<86..626)
                     /locus_tag="PLF56_02905"
                     /inference="COORDINATES: protein motif:HMM:NF023697.2"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="DUF3618 domain-containing protein"
                     /protein_id="HOQ52543.1"
                     /translation="MTQSNDPDEIRADIERTRAELSHNVDTLGDTVNPKNIAARQVDK
                     VKDTARSIKDSIMGSSDDPYDSGRVGELQSAASSGVSAVGDALSQAPAVAQRKTRGNP
                     LAAGLIAFGAGLLVSSLIPASRKEQQAVGALQEHLEPVKQQAGEVAKEMADHLREPAQ
                     QAAESIKAVATDAADNVKQQ"
     gene            complement(623..1090)
                     /locus_tag="PLF56_02910"
     CDS             complement(623..1090)
                     /locus_tag="PLF56_02910"
                     /inference="COORDINATES: protein motif:HMM:NF018980.2"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_component: GO:0005886 - plasma membrane [Evidence IEA];
                     GO_component: GO:0016020 - membrane [Evidence IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="phage holin family protein"
                     /protein_id="HOQ52544.1"
                     /translation="MTYPDPAQAPAGVPERQSVHRSSALEAEDGRSIGDLLSDVSRNL
                     STLLQQEIALAKAEARQSGTRAGKGIGMFAGAAIGGLLFLVFLSVSAWWGLGLHIGNE
                     WSGLIVAVVWAIVAAALALAGKKELERVRGLPRTTETIAKIPNAVRGHEEDNR"
     gene            complement(1090..1989)
                     /locus_tag="PLF56_02915"
     CDS             complement(1090..1989)
                     /locus_tag="PLF56_02915"
                     /inference="COORDINATES: ab initio
                     prediction:GeneMarkS-2+"
                     /note="Derived by automated computational analysis using
                     gene prediction method: GeneMarkS-2+."
                     /codon_start=1
                     /transl_table=11
                     /product="hypothetical protein"
                     /protein_id="HOQ52545.1"
                     /translation="MTHTPSEYDETPAATTGVHGDSTSSLNEFAPLSQSSAADDGYST
                     GSSGSGTMDTAKDEAGQVAGAGVQAAKEVASTAKGEAATVLAETKQQAASLFETARDE
                     VRSQSGVQQQRLASGLHSLAQELGGMASGAEQQGQLSDLMKQAAQKGGEIAHWLETHE
                     PADLLREVQSYARRRPVAFLALCGLAGIVAGRVARSAAAANTSLDSGKSEGHGARPAL
                     DVGTELQQPGSQRYGHSIATADVAATADRGYSDRLGSPGLFGSENDELQASAVPGSLG
                     AGTRGPGPGVDRLDDPSTRPGAM"
     gene            2241..4460
                     /locus_tag="PLF56_02920"
     CDS             2241..4460
                     /locus_tag="PLF56_02920"
                     /EC_number="2.7.1.11"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_016668497.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="6-phosphofructokinase"
                     /protein_id="HOQ52546.1"
                     /translation="MVDSIGEGKRIGILTSGGDAQGMNAAVRAVVRTAITLGAEVYAI
                     FEGYQGMIDGGDGIRRFGWDDVGNVLHRGGTVIGTFRCQRFRERDGRRIAARNLVHSG
                     IDRLVVIGGDGSLSGLDAFRAEWPELLAELVAAGEIEQSVADAHPALMIAGLVGSIDN
                     DLVGTDMTIGADSALHRIVDAIDDLASTAASHQRSFVVEVMGRHCGYLALMAAVAGGC
                     DFVLTPEAPPAEGWQERMCGELARGRAAGRRDSIVVVAEGATDREGRPITSEQVRQII
                     EDRLGEDARVTILGHVQRGGTPSAFDRWCSTWLGFEAAKALLEADGGSEGHVFGIRGN
                     RVAAVPLMEAVRNTREVPVLIEQGDYAAAMAKRGDSFVTMAKLFGELTEPSRISVAEG
                     AKRIGLIHAGGLAPGMNTAARAAVRLGISRGYTMLGIRDGFPGLREGRVAELSWGDVE
                     AWTADGGAKLGLRRTIPTIEELYPISRQLEDHHVDALLMIGGWNGYQAVHLLHAERDR
                     YPAFRMPMVCLPASIDNNLPGSELAVGADTALNVIVEAIDRIKLSASATKRSYIVETM
                     GRYCGYLTLMGGLAGGSERVYLHEEGITLERLNDDVKWVRSSFAAGRSLFLAVRNERA
                     NEYYTTDVIARLLQAEAAGLYDVRQGVLGHQQQGGSPSPFDRLMATRLAGHALEKIAD
                     QFAVGSDGSYLVGLAGAKINDVPIETMMTLMDRTYRRPRQQWWLQLREVVRAVAEEP"
     gene            complement(4512..6290)
                     /gene="treZ"
                     /locus_tag="PLF56_02925"
     CDS             complement(4512..6290)
                     /gene="treZ"
                     /locus_tag="PLF56_02925"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_016669998.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_function: GO:0016798 - hydrolase activity, acting on
                     glycosyl bonds [Evidence IEA];
                     GO_process: GO:0005992 - trehalose biosynthetic process
                     [Evidence IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="malto-oligosyltrehalose trehalohydrolase"
                     /protein_id="HOQ52547.1"
                     /translation="MLHQYTVWAPTAQRVDLVLGADGDRVEPMRDVGDGWWALDAEQG
                     DGRYAFALNGGAPLPDPRSMRQPDGVHAPSAVVDTGTFAWTDDDWAGKPLKGSVIYEL
                     HIGTFTDEGTFDAALAHLQHLADLGIDVIELMPVASFPGRNGWGYDGVDLYSVHEPYG
                     GPDGLYRFVDAAHAHGLAVWLDVVYNHFGPDGNYLPQYGPYFTERHQTPWGVAVNLDG
                     PGSDQVRAYFVDNLRQWLRDYHLDGLRLDAVHALHDEEAVHLLEELALVAAVISAATG
                     IPRVLVAESDRNDPATVAPRGLGGAGGMGLDGQWADDVHHALHVALTREEQGYYADFA
                     DPQALTKVLGHSPFFHDGTFSSFRGRRHGRPVEPSITPGWRFVASLQTHDQVGNRAMG
                     ERLSHLAGIGRAAIGAALLLTSPYTPMLFMGEEFGASTPWQFFTDHQHEALVKSIREG
                     RQAEFAEHGWATQVPDPQDPGTVEASTLNWREVGQGEHAQLLEWYRTLIRLRRQLPDL
                     RSANLGEGSLARVDDLLLVRRGEVNLLANLGDQPGSLAVALDAELLASFGEVALDDGE
                     VQLGPTSVGLLLAPPPTGTIPVVITD"
     gene            complement(6290..8731)
                     /gene="treY"
                     /locus_tag="PLF56_02930"
     CDS             complement(6290..8731)
                     /gene="treY"
                     /locus_tag="PLF56_02930"
                     /inference="COORDINATES: protein motif:HMM:TIGR02401.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_function: GO:0047470 - (1,4)-alpha-D-glucan
                     1-alpha-D-glucosylmutase activity [Evidence IEA];
                     GO_process: GO:0005992 - trehalose biosynthetic process
                     [Evidence IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="malto-oligosyltrehalose synthase"
                     /protein_id="HOQ52548.1"
                     /translation="MTPSTPARAFTGTYRLQLHAGFTLDDARAAVPYLADLGVSHLYL
                     APILSAVPGSVHGYDVLDHTAVNPEIGGRPALERLAQACHEHDLGIVVDIVPNHMALV
                     APQWRNAPVWDVLRHGRDSAFAHWFDVDWHHLNGRFGLPILGGTLADELAAGTLHVDT
                     GRPDEGPAAGLPVLRYYDHVLPLRDDVPLRDDMPLRDGVPVRDGVPPTSDLADLLDAQ
                     HWLLASHKQAANVLNYRRFFEVDQLVAIRVELPDVFDATHALVLDLHHSGVIDGFRVD
                     HPDGLADPAGYLQRLADACRPGTPIWVEKILEGHERLPQWACTGTTGYDAAAALDTAL
                     VDPATVAAVTEAWARTGGEPSLELCTEQSKGQVLDQLLQPELNRLHRAAIAALPDLDP
                     SALRANLAELLVELHVYRAYVTPGQEPDPHLLAPLHQALAHVGERHPDLRATASELVA
                     VLQRPDTAPGDPDAAADLCIRFQQTTGPVMAKGIEDTTFYRWHRLCALNEVGFDPVTG
                     ERPGPADLHAWAAHQASHWPLGMTTLSTHDTKRSEDVRARLIALAGDADAWTALSRLA
                     SEAAERHGVDAPTAHLVWQSIAGTAPITAERLDAYLTKAIREAKRHTSWLQPDTDYEK
                     RVIGLGRSILDGDALGEAISTAVKANEGAIRTVTLAQKLVQLTLPGVPDTYQGAEQVS
                     LTLVDPDNRSNVDLAERAARLRRLDDGGTAGSLDDEKLLVTARALRLRRERPGLFATD
                     HRPLALDDTLLGFARGDDVVVVVQRLAHAAPSGSVTLAAGNWTDVLTGYRYETSVPAV
                     DLLATLPVALLVREP"
BASE COUNT         1259 a         2987 c         3245 g         1401 t
ORIGIN      
        1 ggttctagtt ggagccggcg ctctggacgg tgtccttcgc gtcctgggcc cggctggcga
       61 cgtcgtcggt tgcggnnnnn nnnnncctgc tgcttgacgt tgtcggcggc gtcggtggcc
      121 actgccttga tcgactccgc cgcctgctgg gccggctcgc gcagatggtc cgccatctct
      181 ttggcgacct cgccggcctg ctgcttcacg ggctcgaggt gctcctgcag ggcgccgacg
      241 gcctgttgct cctttcgact ggccggaatc agggacgaca ccagcaggcc ggcgccgaag
      301 gcgatcagcc cggccgccaa cgggttgccc cgggtcttgc gctgcgccac cgcgggtgcc
      361 tgactgagcg catcgcccac agcgctcacg ccgctggagg cggccgattg cagttcaccc
      421 acccggcccg agtcgtacgg gtcgtcggac gagcccatga tcgagtcctt gatcgaccgg
      481 gcggtgtcct tgaccttgtc gacctggcgg gcggcgatgt tcttcggatt gacggtgtcg
      541 ccgagcgtgt ccacgttgtg gctcagctcg gctcgggtgc gctcaatgtc ggcgcggatc
      601 tcatcgggat cgttactctg cgtcatcggt tgtcctcctc atgacctcgt acagcgttgg
      661 gaatcttggc aatggtttcg gtggtccggg gcaggccacg gacgcgttcg agttccttct
      721 tgccggccag ggccagcgcc gcggcgacga tcgcccagac cacggcgacg atcaggcccg
      781 accactcgtt gccgatgtgc agcccgaggc cccaccaggc gctcaccgac aggaacacca
      841 ggaagagcag gccgccgatg gccgcgccgg cgaacatacc gatgcccttg cccgcgcggg
      901 ttccggactg gcgggcctcc gccttcgcca gggcgatctc ctgctgcaac agggtggaca
      961 ggttgcggct cacgtccgac aacaggtcgc cgatgctacg gccgtcctcg gcctccagcg
     1021 ccgagctgcg atggacgctc tgccgctcgg gcaccccggc cggggcctgt gccggatcgg
     1081 gatacgtcat cacatcgctc cgggacgggt ggaaggatcg tccagccggt caacacccgg
     1141 accggggccg cgggtaccgg cgccgagcga tccgggcacc gctgaagcct ggagttcgtc
     1201 gttctcggat ccgaacaggc cgggggagcc gagccggtcg gagtagccgc ggtcggccgt
     1261 ggcggccacg tcggccgtgg cgatgctgtg gccgtaccgc tgcgaacccg gctgctgaag
     1321 ctccgtgccc acatcgagag cgggcctggc cccatggcct tccgacttgc cggagtccag
     1381 gctcgtgttg gccgcggccg cgctgcgtgc gacccgtccg gccacgattc cggcgagtcc
     1441 gcacagcgcc aggaaggcca ccggccggcg gcgcgcgtag gactgcacct cacgcagcag
     1501 gtcggcaggt tcatgggttt ccagccaatg tgcgatctcg ccacccttct gcgccgcctg
     1561 cttcatcagg tcactgagtt gaccctgctg ctcggcgccg gacgccatgc cgcccagctc
     1621 ctgggcgagc gagtgcaggc ccgaggccag ccgctgctgc tggacgccgg actgtgagcg
     1681 cacctcgtcc ctcgccgtct cgaacaggga ggcggcctgc tgcttggtct cggccagcac
     1741 ggtggcggcc tcgcccttgg ccgtggacgc cacctccttg gccgcctgga cgcccgcacc
     1801 ggcgacctga cccgcttcgt ccttcgcggt gtccatcgtg ccgcttccgg acgagccggt
     1861 ggagtaaccg tcgtctgcgg cggacgactg gctgagcggg gcgaactcgt tcaagctgct
     1921 ggtcgaatcg ccgtggaccc cggtcgttgc agccggggtt tcgtcgtact cggatggggt
     1981 atgtgtcatg acgaagctcc tagtgggctc gtgacgtttc ctccgcaccg acggcggagc
     2041 tggccatgac gacactaact tacggtgtaa gtctgcaatg cacccccttg ggtgccttca
     2101 gcccttgctc cggtacatcg aaactcgcgt tcgtcgagat cctccgcgcc gcgctgccgc
     2161 ctagcggcgc ctagcggtcg tcaacgatgc aggtcgtgac atcggtgacc gtggaggctt
     2221 tcgtgcgcaa ggatgatcac atggtcgaca gcatcggtga aggcaagcgc atcggcattc
     2281 tgaccagtgg cggcgacgcc cagggcatga acgcggcggt acgagccgtc gtccgaaccg
     2341 cgatcaccct gggtgccgag gtgtacgcca tcttcgaggg ctaccagggc atgatcgatg
     2401 gcggcgacgg catccgccgc ttcggctggg acgatgtcgg caacgtgctg caccgcggcg
     2461 gcaccgtgat cggcaccttc cgctgccaac gctttcgcga gcgtgacgga cggcgcatcg
     2521 ccgcccgcaa cctggtgcac tccggcatcg accggttggt ggtgatcggc ggcgacggca
     2581 gcctgtcggg gctggacgcc tttcgcgccg agtggcccga gctgctcgcc gaactggtag
     2641 cggccggcga gatcgagcag tccgtcgccg acgcgcaccc ggcgctgatg atcgcgggcc
     2701 tggtcggcag catcgacaac gacctggtcg gcaccgacat gacgatcggc gccgacagcg
     2761 ccctgcatcg catcgtcgat gcgatcgacg acctggccag caccgccgcc agtcaccagc
     2821 gcagtttcgt ggtcgaagtg atgggtcggc actgcggcta cctggcgctg atggccgcgg
     2881 ttgcgggtgg ctgtgacttc gtgctgactc ccgaggcacc ccctgcggag ggctggcagg
     2941 agcgcatgtg tggcgagctg gcccgcggcc gggcggccgg acgccgcgat tccatcgtgg
     3001 tggtcgccga gggtgccacc gaccgcgaag gccgcccgat cacgtccgaa caggtgcggc
     3061 agatcatcga ggaccggctc ggcgaagacg cccgggtcac gatcctgggg cacgtgcagc
     3121 gcggcggcac gcccagtgcc ttcgaccgct ggtgctccac gtggctgggt ttcgaggcgg
     3181 ccaaggcgct gctcgaagcg gacggcggca gcgagggcca cgtgttcggc atccgcggca
     3241 accgggtcgc ggccgttccc ctgatggagg cggtgcgcaa cacccgcgag gtgccggtgc
     3301 tgatcgagca gggcgactac gcggccgcca tggccaagcg cggtgactcg ttcgtcacca
     3361 tggccaagct gttcggcgaa ctgaccgagc cgagccggat cagcgtggcc gagggcgcca
     3421 agcgcatcgg cctcatccac gccggggggc tggcgccggg catgaacacg gccgcgcgcg
     3481 ccgcggtgcg cctgggcatc agccgcggct acaccatgct gggcattcgg gacgggtttc
     3541 cgggcctgcg cgaggggcgc gtcgccgaac tcagttgggg cgacgtcgag gcctggacgg
     3601 cggacggcgg cgcgaagctc ggcttgaggc ggaccatccc caccatcgaa gagctgtacc
     3661 cgatcagccg ccaactcgag gatcaccacg tggacgcgtt gctgatgatc ggcggctgga
     3721 acggctacca ggccgtccat ctgctgcatg ccgagcgcga ccgctacccg gcattccgca
     3781 tgccgatggt gtgcctgccg gccagcatcg acaacaacct gcccggctcc gaactcgccg
     3841 tcggcgccga cacggcactg aacgtgatcg tcgaggcgat cgaccgcatc aagctgtcgg
     3901 cctctgcgac caaacgcagc tacatcgtcg agacgatggg gcgctactgc ggctacctga
     3961 ccctgatggg tgggctggcc ggcggttcgg agcgggtgta cctgcacgag gagggcatca
     4021 ctctcgagcg gctcaacgac gacgtgaagt gggtgcgcag ttcgttcgcg gccggccgct
     4081 cactgtttct ggcagtgcgt aacgaacgtg ccaacgagta ctacaccacc gacgtgatcg
     4141 cgcggttgct gcaggccgag gcggccgggc tctatgacgt gcgacaaggg gtgttggggc
     4201 atcaacagca gggcggctcg cccagcccgt tcgaccggct gatggccacc cggctggcgg
     4261 ggcacgccct ggagaagatc gccgatcagt tcgccgtcgg ttcggacggc agctacctgg
     4321 tcggcctggc cggcgccaag atcaacgacg tgccgatcga gaccatgatg acgctgatgg
     4381 atcgcacgta tcggcgtccg cggcagcagt ggtggctgca gctgcgcgag gtcgtccggg
     4441 ccgtggcgga ggagccgtag ccggtctcga cacgcttgac cagcgcgtcg cgagtcgtcc
     4501 agtaatcgac gctagtcggt gatcaccacg ggaatagtgc cggtcggcgg cggggccagc
     4561 agcaggccca cgctggtcgg gcccagctgc acctcaccgt cgtccagggc gacctcgccg
     4621 aaggatgcca gcagctcggc gtccagcgcc accgcgaggc tgcccggctg atcgcccaga
     4681 ttggccagca ggttcacctc gccgcgacgc accaacagca ggtcgtccac gcgggccagc
     4741 gacccttcgc ccagattggc cgaacgaagg tcgggcagtt gccggcgcag ccggatcagg
     4801 gtgcggtacc actccagcag ttgtgcgtgc tcgccctgac ccacctcgcg ccagttcaac
     4861 gtggaggcct cgaccgtgcc cggatcctgc gggtccggca cctgggtggc ccagccgtgc
     4921 tccgcgaact cggcctgccg gccctcccgg atcgacttga ccaacgcctc gtgctggtgg
     4981 tcggtgaaga actgccaggg cgtggacgca ccgaactctt cgcccatgaa cagcatgggc
     5041 gtgtaggggg aggtgagcag cagggcggcg ccgatcgcgg cgcgtccgat gccggccagg
     5101 tggctgagcc gctcgcccat cgcgcggttg ccgacctgat cgtgggtctg cagcgaagcc
     5161 acgaagcgcc agccgggtgt gatcgacggt tcgaccggac ggccgtgccg ccgtccgcgg
     5221 aacgacgaga acgtgccgtc gtgaaagaag ggggagtgcc cgagcacctt ggtcagtgcc
     5281 tgcgggtcgg cgaagtcggc gtagtacccc tgctcttcgc gggtcagggc cacgtgcagg
     5341 gcgtggtgca catcgtcggc ccactggccg tccaggccca tgccgccggc cccacccagc
     5401 ccgcgcgggg caacggtcgc cgggtcgttg cggtcggatt cggccaccaa cacccgcggg
     5461 attccggtgg cggcactgat caccgcggcc accaaggcga gctcttcgag cagatgcacc
     5521 gcctcttcgt cgtgcagggc gtgcacggcg tcgaggcgca gcccgtccag gtggtagtcc
     5581 ctcagccact ggcgcaggtt gtcgacgaag taggcacgca cctggtcgct gccggggccg
     5641 tccaggttga ccgcgactcc ccagggcgtc tggtgccgtt cggtgaagta gggaccgtac
     5701 tggggcaggt agttgccgtc ggggccgaag tggttgtaga ccacgtccag ccagaccgcc
     5761 aggccgtgcg cgtgcgcggc atcgacgaag cggtacagcc cgtccgggcc gccgtagggc
     5821 tcgtgcaccg agtacaggtc gacgccgtcg tagccccacc cgttgcggcc ggggaacgag
     5881 gccaccggca tcagttcgat cacgtcgatg ccgaggtcgg ccaagtgctg caggtgggcg
     5941 agcgcggcat cgaacgtgcc ttcgtcggtg aaggtgccga tgtgcaactc atagatcacc
     6001 gaccccttga gcggcttacc ggcccagtcg tcgtcggtcc aggcgaacgt gccggtgtcg
     6061 accaccgcac tgggggcgtg gacgccgtcc ggctgccgca tgctgcgcgg gtccggcagg
     6121 ggcgcgccac cgttcagggc gaaggcgtag cgcccgtcgc cctgctcggc atccagggcc
     6181 caccagccgt cgccgacgtc gcgcatcggc tcgacccggt cgccgtcggc gcccaacacc
     6241 aagtcgacgc gttgggccgt cggggcccac accgtgtact ggtgcagcat cacggctccc
     6301 ggaccagtaa ggcgaccggc agggtggcca gcagatcgac cgccggcacc gaggtctcgt
     6361 agcggtagcc ggtcagcacg tcggtccagt tgccggccgc gagcgtgacg ctgccggacg
     6421 gtgcggcgtg cgcgaggcgt tgcaccacca cgaccacgtc gtcgccacgg gcgaagccga
     6481 gcagggtgtc gtccagagcg agcggacgat ggtcggtggc gaacagtccc ggccgctcgc
     6541 ggcgcagccg tagcgcgcgg gcggtgacca gcagtttttc gtcgtccaga gatccggcgg
     6601 tgcccccatc gtccagtcgc cgcagccggg cggcgcgctc ggcgaggtcg acgttcgagc
     6661 ggttgtcggg atcgaccagg gtgaggctga cctgctcggc gccctggtag gtgtcgggca
     6721 cgccgggcag ggtgagctgc accagtttct gcgcgagcgt gacggtgcgg atcgcgcctt
     6781 cgttggcctt gaccgcggtg ctgatcgcct cgccgagcgc gtcaccatcg aggatcgatc
     6841 ggcccaaccc gatcacccgt ttctcgtagt cggtgtcggg ttgcagccaa ctggtgtgcc
     6901 gtttcgcctc gcggatcgcc ttggtcaggt aggcgtccag ccgctcggcg gtgatcgggg
     6961 ccgtgccggc gatgctctgc cagaccaggt gggcggtggg agcgtccaca ccgtgccgct
     7021 cagcggcctc ggaggcgagc cggctgagcg cggtccaggc gtcggcgtcg ccggccaggg
     7081 cgatcaggcg ggcccggacg tcctcgctgc gcttggtgtc gtgggtcgac agggtggtca
     7141 tgccaagggg ccagtgggac gcctggtggg cggcccaggc gtgcaggtcg gccgggccgg
     7201 gacgctcgcc ggtgaccggg tcgaagccca cctcgttgag cgcgcacagc cggtgccagc
     7261 gatagaacgt ggtgtcttcg atgcccttgg ccatcaccgg gccggtggtc tgctggaagc
     7321 ggatacagag gtcggcggcg gcgtccggat cgccgggtgc cgtgtcgggc cgctggagca
     7381 cggcgacgag ctcggacgcc gtggcgcgca ggtcggggtg gcgctcaccg acgtgcgcca
     7441 gggcctggtg caggggggcg agcagatggg gatcgggctc ctggcccggg gtgacatagg
     7501 cccggtagac gtgcagttcg accagcagtt cggctaggtt tgcgcgcagt gcgctcgggt
     7561 cgaggtcggg cagcgcggcg atcgccgcgc ggtgcaaccg gttcagctcc ggctgcagca
     7621 actgatcgag cacctggccc ttgctctgct cggtgcacag ctccagggat ggctcgccgc
     7681 cggttcgtgc ccaggcctcg gtgaccgccg ccaccgtcgc agggtcgacc agcgccgtgt
     7741 cgagcgcggc ggcggcgtcg tatccggtgg tgccggtgca ggcccattgg ggcagccgct
     7801 cgtggccctc gaggatcttc tccacccaga tcggggtgcc cggacggcag gcgtccgcga
     7861 gccgctgcag atagcccgcc gggtcggcca ggccgtcggg gtggtcgacc cggaagccgt
     7921 cgatgacgcc gctgtgatgc aggtcgagca cgagggcgtg ggtggcgtcg aagacgtccg
     7981 gcagttcgac ccggatcgcc accagttggt cgacctcgaa gaagcgacgg tagttgagca
     8041 cgttcgcggc ctgcttgtgc gaggcgagca gccagtgctg ggcgtccagc aggtcggcca
     8101 ggtcggatgt tgggggtacg ccgtcgcgta ccggtacacc gtcgcgcagc ggcatgtcgt
     8161 cgcgcagcgg cacgtcgtcg cgcagcggca gcacgtggtc gtagtagcgc agcaccggca
     8221 gcccggcggc ggggccctcg tcggggcgtc cggtgtcgac gtgcagtgtg ccggcggcca
     8281 gctcgtcggc cagcgtgccg cccaggatcg gcagcccgaa gcgtccgttc aggtggtgcc
     8341 aatcgacgtc gaaccagtgt gcgaaggccg agtcgcgacc gtgccgcagc acgtcccaga
     8401 ccggggcgtt gcgccattgc ggcgcgacca gcgccatgtg gttgggcacg atgtcgacga
     8461 cgatgcccag atcatgctcg tggcaggcct gggccagtcg ttccagcgcc gggcgtccgc
     8521 cgatctcggg gttcaccgcg gtgtggtcga gcacgtcgta gccgtgcacc gagccgggca
     8581 ccgcgctcag gatcggcgcc aggtacaggt ggctcacgcc gaggtcggcc aggtacggca
     8641 cggcggcgcg ggcatcgtcc agggtgaagc cggcgtgcaa ctgcagccgg taggtgccgg
     8701 tgaacgcgcg ggccggggtg gagggggtca cccggatcat tgtggcctgc cgcgggcgtg
     8761 cgctgccacc cttctcaccg cgcagtgccg tgtcgctcac tggcctgctg ttcggcgccg
     8821 cgaaaacggg ttacgtgacg cgggcgactc gtcactaagg ttgcggggtg gcatttctgc
     8881 tggcgggcga ggctctgcac ct
//
LOCUS       DAOSUG010000053         8840 bp    DNA     linear   ENV 10-JUN-2024
DEFINITION  MAG TPA_asm: Micropruina sp. isolate AMR_MDS_2195
            NODE_4192_length_8840_cov_4.510985, whole genome shotgun sequence.
ACCESSION   DAOSUG010000053 DAOSUG010000000
VERSION     DAOSUG010000053.1
DBLINK      BioProject: PRJNA925477
            BioSample: SAMN32864262
            Sequence Read Archive: SRR4236673
KEYWORDS    WGS; ENV; Metagenome Assembled Genome; MAG; Third Party Data; TPA;
            TPA:assembly.
SOURCE      Micropruina sp. (wastewater metagenome)
  ORGANISM  Micropruina sp.
            Bacteria; Bacillati; Actinomycetota; Actinomycetes;
            Propionibacteriales; Nocardioidaceae; Micropruina.
REFERENCE   1  (bases 1 to 8840)
  AUTHORS   Abdulkadir,N., Saraiva,J.P., Zhang,J., Stolte,S., Gillor,O.,
            Harms,H. and Rocha,U.
  TITLE     Genome-centric analyses of 165 metagenomes show that mobile genetic
            elements are crucial for the transmission of antimicrobial
            resistance genes to pathogens in activated sludge and wastewater
  JOURNAL   Microbiol Spectr 12 (3), e0291823 (2024)
   PUBMED   38289113
REFERENCE   2  (bases 1 to 8840)
  AUTHORS   Abdulkadir,N.
  TITLE     Direct Submission
  JOURNAL   Submitted (24-MAR-2023) Environmental Microbiology, Helmholtz
            Center for Environmental Research -UFZ, Permoserstr 15, Leipzig
            04318, Germany
COMMENT     The annotation was added by the NCBI Prokaryotic Genome Annotation
            Pipeline (PGAP). Information about PGAP can be found here:
            https://www.ncbi.nlm.nih.gov/genome/annotation_prok/
            
            ##Genome-Assembly-Data-START##
            Assembly Method        :: SPAdes v. 3.11.1
            Genome Representation  :: Full
            Expected Final Version :: Yes
            Genome Coverage        :: Not Applicable
            Sequencing Technology  :: Illumina MiSeq
            ##Genome-Assembly-Data-END##
            
            ##Genome-Annotation-Data-START##
            Annotation Provider               :: NCBI
            Annotation Date                   :: 05/04/2023 04:57:10
            Annotation Pipeline               :: NCBI Prokaryotic Genome
                                                 Annotation Pipeline (PGAP)
            Annotation Method                 :: Best-placed reference protein
                                                 set; GeneMarkS-2+
            Annotation Software revision      :: 6.5
            Features Annotated                :: Gene; CDS; rRNA; tRNA; ncRNA
            Genes (total)                     :: 2,776
            CDSs (total)                      :: 2,750
            Genes (coding)                    :: 2,714
            CDSs (with protein)               :: 2,714
            Genes (RNA)                       :: 26
            tRNAs                             :: 24
            ncRNAs                            :: 2
            Pseudo Genes (total)              :: 36
            CDSs (without protein)            :: 36
            Pseudo Genes (ambiguous residues) :: 0 of 36
            Pseudo Genes (frameshifted)       :: 2 of 36
            Pseudo Genes (incomplete)         :: 32 of 36
            Pseudo Genes (internal stop)      :: 2 of 36
            ##Genome-Annotation-Data-END##
FEATURES             Location/Qualifiers
     source          1..8840
                     /organism="Micropruina sp."
                     /mol_type="genomic DNA"
                     /submitter_seqid="NODE_4192_length_8840_cov_4.510985"
                     /isolate="AMR_MDS_2195"
                     /isolation_source="Activated sludge of municipal
                     wastewater treatment plants"
                     /db_xref="taxon:2737536"
                     /environmental_sample
                     /geo_loc_name="Canada"
                     /lat_lon="49.25 N 123.1 W"
                     /collection_date="2015-06-16"
                     /metagenome_source="wastewater metagenome"
                     /note="metagenomic"
     gene            complement(<1..2319)
                     /locus_tag="PLF56_02935"
     CDS             complement(<1..2319)
                     /locus_tag="PLF56_02935"
                     /inference="COORDINATES: protein motif:HMM:NF013239.2"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_function: GO:0004553 - hydrolase activity, hydrolyzing
                     O-glycosyl compounds [Evidence IEA];
                     GO_process: GO:0005975 - carbohydrate metabolic process
                     [Evidence IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="glycoside hydrolase family 31 protein"
                     /protein_id="HOQ52549.1"
                     /translation="MTLPEHLILPTTPHAVPEAQVVGENYRITVLSNALFRLEYSSNG
                     SFEDRATQMVINREFEPVEFTVHRTKHGIEIVTDTVHLSYDERPFSPGGLYAHLAGGS
                     GHRRTWRYGERQQLWGGGDANLGGTARTLDEVDGPADLEDGVLSFLGSAVLDDSTTMA
                     LESDGWIGTRTPGNTDVYLFAHGDAYQQALDDFHRLTGHQPLLPRWALGNWWSRYHPY
                     SDAEYRGLMERFDAEGVPFSVAVLDMDWHLVDVPPQYGTGWTGYTWNRELFPDPPDLL
                     AWLHDRGLRVALNVHPADGVRAFEEPYRRIADALGIDPATEQPVDFDIAEQSFLRAYL
                     EQVHHPLEDDGVDFWWLDWQQGTHTSISGLDPLWLLNHYHYLDSARRGRRPITFSRYA
                     GPGSHRYPVGFSGDTITSWPSLQFQPRFTATASNIGFGWWSHDIGGHMFGYRDNELAT
                     RWYQLGTFSPINRLHSSLSQFQGKEPWNYPPEYASVMVESLRLRHRLVPYLYTMNERA
                     HRTNRSVCRPLYHVDQKADLLFQRGYGSPNTFLFGSRLLVAPITSPVDPVTGLASVTT
                     WLPEGDWVDFFTDVAYTGGRTLHLHRPLAGYPVLARTGAVVPLTAPDDYGIGNPDAFE
                     VHVFAGARGSFTLYEDDDAAEPRAVRTHLEWDDQAGVFTVAAAQGALEVVPEFRSWTL
                     ILHGIGAAEVDHGRATPEGGRLRVDLGAVETAVGAAVRLRERMAPSPSLRERLFAVLH
                     DAQCEYLTKERTWGAINAPGDTLAVLRRLDGLQ"
     gene            complement(2713..3450)
                     /locus_tag="PLF56_02940"
     CDS             complement(2713..3450)
                     /locus_tag="PLF56_02940"
                     /inference="COORDINATES: protein motif:HMM:NF014420.2"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_process: GO:0055085 - transmembrane transport [Evidence
                     IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="energy-coupling factor transporter transmembrane
                     component T"
                     /protein_id="HOQ52550.1"
                     /translation="MSVLARLNPLTPLALITALGIGVSFLFAPLPTLALHLSVVTLLL
                     AAGAGLRGVVISHLTFTPFAVGVLATNAVSRPGAVLAHAGPLMITDEGVAVGMALALR
                     VLVVAVPAVAVASVLDPTRLVTAAMQHLRLTPSVGFAVLTAHRMLGAMPQQWSTLLAA
                     GRSRAPLDRRGRPKLGVRGYGRAAFGLLVGGIRRGEQIAGALQVRGLRSTRRTMRLQV
                     PFVRLDAVAAVVAMVVCGTATGLVLVL"
     gene            complement(3447..4946)
                     /locus_tag="PLF56_02945"
     CDS             complement(3447..4946)
                     /locus_tag="PLF56_02945"
                     /inference="COORDINATES: protein motif:HMM:NF012235.2"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_function: GO:0005524 - ATP binding [Evidence IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="ATP-binding cassette domain-containing protein"
                     /protein_id="HOQ52551.1"
                     /translation="MSDVLRVRGLTVHPRHGRPLLRGIELSIEPGEQVLLAGASGSGK
                     STLLRLIAGLTPGPHVRIEGSIEVSGDDVLGRPVTAPPLPIGWLPQDPAAAVCLPRVA
                     DDVAFGCESRCWGVGEIEAAVLRSLLLVDAADWHDRDTVTLSAGQAQRVGLAGATAPA
                     PALLLLDEPTSMLDPEALRRVRAAVAAVAETPAPPALLLVEHRLDEWADGAGLPGRVV
                     LLEEGGIIADGPAEQVWRDHGARLIAAGCHVPWPVERRHRPAPPTAPTAPPAAEPVLA
                     TRALAVGHDRTPIVTGATVAVHAGEVVALIGRNGAGKTTLLQTLAGIRPPLAGRIELP
                     SGRPALIFQSPEQQFLASTVRDELAFDGAPDDQVQTSLAELRLTGAAGLSVHRLSGGE
                     QRRLSVGTVLLGRRQAVLADEPTFGLDRDGVEWTARAFRDAAARGRGVLLTSHDLRFV
                     LEVADRTYLIHQGEVLAGGPTAAVLADPLLARAGLPLPAAVREALGVAA"
     gene            complement(4943..5560)
                     /locus_tag="PLF56_02950"
     CDS             complement(4943..5560)
                     /locus_tag="PLF56_02950"
                     /inference="COORDINATES: protein motif:HMM:NF021348.2"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="ECF transporter S component"
                     /protein_id="HOQ52552.1"
                     /translation="MSTSGEGWLSRWQTRDIMLVVVLGVVFGFVYWVLVQAWVALSLL
                     AGPFGDLTQHVLLGGWLIVAPLAVAITRRPGAGIVAEVIASIIEVVFLGSPVGPLLLV
                     AAFLQGLGSELPFFLTRYRRFGWGVYAASGALGAFAVFWFSALRAGWFGSDLLLLRLG
                     MQVTSGVILGGLLARLLVMALQRTGVLDSFAIGALDDHPPASEPA"
     gene            complement(5557..6225)
                     /locus_tag="PLF56_02955"
     CDS             complement(5557..6225)
                     /locus_tag="PLF56_02955"
                     /inference="COORDINATES: protein motif:HMM:NF019246.2"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="YkoF family
                     thiamine/hydroxymethylpyrimidine-binding protein"
                     /protein_id="HOQ52553.1"
                     /translation="MTTITPDRPASTGRAIHPAPDPITQAATSGVGARITVAVMADDY
                     VRLITQALAAVNREGLQLDTDKISTFVRGSEQRLAEYLRDLIAAVAASGHHVVAHLML
                     SRGCPGEVCGAVPPPALSSVRLDPSGVAVQAQWSLYPLADAGAPAPEDGSDHLAPVWA
                     AIERARHDATFLRAEHFVTTLAGDLSDVLRTVFATWLAAGSAVPHTVTHVTIGVNSPS
                     ENLR"
     regulatory      complement(6274..6385)
                     /regulatory_class="riboswitch"
                     /inference="COORDINATES: nucleotide
                     motif:Rfam:14.4:RF00059"
                     /inference="COORDINATES: profile:INFERNAL:1.1.4"
                     /note="TPP riboswitch; Derived by automated computational
                     analysis using gene prediction method: cmsearch."
                     /bound_moiety="thiamine pyrophosphate"
                     /db_xref="RFAM:RF00059"
     gene            6718..8061
                     /locus_tag="PLF56_02960"
     CDS             6718..8061
                     /locus_tag="PLF56_02960"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_017936690.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_function: GO:0005524 - ATP binding [Evidence IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="PhoH family protein"
                     /protein_id="HOQ52554.1"
                     /translation="MPTTASTSRRSTRSASATDVRTYVIDTSVLLSDPHALRNFDEHE
                     VIVPIVVITELEAKRDHPELGYFARTALRYLDELRVANGRLDLPLRVNEAGGTLHVEL
                     NHSDQSLLPAGFRLGDNDSRILAVALNYSDEGREVVLVSKDVPLRVKASAVGLLAEEY
                     LHELPADSGWTGMAEVDVAQSSIETLYATGAAIVPEAVDLPCHSGVVLHAAGSSALGR
                     VTPEGGVKLVRSDRDCFGIHGRSAEQRVALDLLLDPAVGIVSLGGRAGTGKSALALCA
                     GLESVLERQQHAKVIVFRPLYAVGGQELGYLPGTEGEKMSPWAQAVFDTLGSVTNRNV
                     IDEVLARDMLEVLPLTHIRGRSLHDAFVIVDEAQSLERNVLLTVLSRIGQNSRVVLTH
                     DVAQRDNLRVGRHDGVVAVVEKLKGHPLFAHVTLTRSERSPIAALVTDLLEDVGI"
     gene            8290..8679
                     /locus_tag="PLF56_02965"
     CDS             8290..8679
                     /locus_tag="PLF56_02965"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_002526006.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="hypothetical protein"
                     /protein_id="HOQ52555.1"
                     /translation="MRTLLRWLLAGSMVFAGLSHLFWARREFQAQVPDWVGETTGLDP
                     DAVVVASGAVEVMLGSALVVLPRDRRRVGAILAAFFVAVFPGNLAQYLDRRDGFGLDT
                     DTKRLVRLFFQPLLVCWALWCTRSEQK"
BASE COUNT         1358 a         3120 c         3119 g         1243 t
ORIGIN      
        1 ctgcaggccg tccagccggc gcaataccgc cagcgtgtcg cccggggcat tgatggcgcc
       61 ccaggtgcgc tccttggtca agtactcgca ctgcgcgtca tgcagcaccg cgaacagccg
      121 ctcgcgtagg ctcggcgacg gtgccatgcg ctcccgcagc cgcacggcgg cacccaccgc
      181 ggtctccacc gcacccaggt cgacccgcag ccgtccgccc tcgggggtgg cccgaccgtg
      241 atcgacctcg gcagccccga tgccgtgcag aatcagcgtc cacgatcgga actcgggcac
      301 cacctcaagc gcaccctggg ccgccgccac cgtgaacacc ccggcctggt cgtcccactc
      361 caggtgcgtc cggacggcgc gtggttcggc cgcgtcgtcg tcctcataca gcgtgaacga
      421 gccgcgcgct cccgcgaaca catgcacctc gaaggcgtcc gggttgccga tcccgtagtc
      481 gtccggggcg gtcaacggca cgactgctcc cgtgcgggcg agcaccgggt agccggccag
      541 cggccggtgc aggtgcaggg tgcgtccgcc ggtgtaggcg acgtcggtga agaagtcgac
      601 ccagtcaccc tcgggcagcc aggtggtcac cgacgccagc ccggtcaccg ggtcgaccgg
      661 cgaggtgatc ggcgcgacca gcagtcgcga gccgaacagg aacgtgttcg gggagccata
      721 cccgcgctga aagagcaggt cggccttctg gtcgacgtga tacaacggcc ggcacaccga
      781 ccggttggtg cggtgtgccc gctcgttcat cgtgtacagg tagggcacca gtcggtgccg
      841 caaccgcagc gactccacca tcaccgaggc gtactcgggg ggatagttcc acggctcctt
      901 gccctggaac tggctcagcg acgagtgcag ccggttgatc ggcgagaagg tgcccaactg
      961 gtaccagcgg gtggccagtt cgttgtcgcg gtagccaaac atgtggccac cgatgtcgtg
     1021 actccaccag ccgaacccga tgttcgaggc ggtggcggtg aaccggggtt gaaactgcag
     1081 gctcggccac gaggtgatgg tgtcgccgga aaagccgacc gggtagcggt gcgaccccgg
     1141 cccggcgtag cgcgagaacg tgatcggacg ccggccgcgg cgcgcgctgt cgaggtagtg
     1201 gtagtggttc agcagccaca gcgggtcgag ccccgagatg gacgtgtgcg tgccctgctg
     1261 ccagtccagc caccagaagt cgaccccgtc gtcctccaac gggtggtgca cctgctccag
     1321 gtaggcccgc agaaagctct gctcggcgat gtcgaagtcg accggctgct cggtcgcggg
     1381 gtcgatgccg agcgcgtccg caatgcgccg gtacggctcc tcgaacgccc ggacgccgtc
     1441 cgccgggtgc acgttgagcg ccacccgcag gccgcggtcg tgcaaccagg cgagcaggtc
     1501 cggcggatcg ggaaacagct cgcggttcca ggtgtagccg gtccagccgg tgccgtactg
     1561 gggcgggacg tccaccaggt gccagtccat gtcgagcacg gccacgctga acggcactcc
     1621 ctcggcatcg aaccgctcca tcaacccccg gtactcggca tcggagtagg ggtgatagcg
     1681 gctccaccag ttgcccaggg cccagcgggg cagcagtggc tggtgcccgg tgaggcggtg
     1741 aaagtcgtcc agggcctgtt ggtaggcgtc gccgtgcgcg aacaggtaga cgtcggtgtt
     1801 gccgggggtg cgggtgccga tccagccgtc ggactcgagc gccatcgtcg tggagtcgtc
     1861 cagcacggcc gagccgagaa agctgagcac cccgtcctcg agatcggccg ggccgtcgac
     1921 ctcgtccagg gtgcgggcgg tgccgcccag gttggcatcg ccgccgcccc acagctgttg
     1981 ccgctcgccg tatcgccagg tgcgccggtg cccggacccc ccggccaggt gcgcgtacag
     2041 cccgccgggg ctgaacggcc gctcgtcgta actcaggtgc acggtgtcgg tgacgatctc
     2101 gatgccgtgc ttggtgcgat gcacggtgaa ctcgaccggc tcgaactcgc gattgatcac
     2161 catctgggtg gcgcggtcct cgaacgagcc gttcgacgag tactccagcc ggaacagcgc
     2221 gttgctcagc acggtgatcc ggtagttctc gcccaccacc tgcgcctcgg gcaccgcgtg
     2281 tggagtggtg ggcaggatca ggtgctcggg caacgtcatg ccacgaccct atcggcgccc
     2341 tcggggaccg tctgcgccga cgtcgctcag gccgtccgcg gcagggtggg cggcctcaca
     2401 gccgtttcat atcgcacttg gacagccggt agtagccggg gccgtaccag gtgcgctttc
     2461 cgttctggtc gagttcgacg gttgatcgcc gccacccgct tgaggtcggc cgtgctgaca
     2521 gtctgctggt cggcggccgc aggtttcggc agcatcgccg ctgtcggaag gctcaccgac
     2581 gcccactgcc gccacgctcc ctggccctgc ttgacgtaca cggacacctc gggacggtcc
     2641 gacaagccgg tggtggcgat cagggtccgc cggtcgccct cgagggacca gccgagcacg
     2701 tcgctgacct ggctacagca ccagcaccag gccggtcgcc gttccgcaca ccaccatagc
     2761 cacgacggcg gccacggcgt ccagtcggac gaagggcacc tgcagccgca tggtgcgccg
     2821 ggtgctgcgc agcccgcgca cctgcagggc gcccgcgatc tgctcgccgc gcctgatgcc
     2881 gccgaccagc aggccgaagg ccgcccgtcc gtagccgcgg acgccgagct tggggcgccc
     2941 gcgccggtcg agcggggcac ggcttcgtcc ggccgccagc agcgtggacc actgctgcgg
     3001 catggcgccc agcatgcggt gcgcggtcag cacggcgaag ccgacgctgg gggtgagccg
     3061 caggtgttgc atggctgcgg tgaccagcct ggtcgggtcg aggacggacg ccaccgcgac
     3121 cgcgggcacc gccaccacca gcacccgcag ggccaacgcc atgccgacag ccacaccttc
     3181 gtcggtgatc atcagaggcc ccgcgtgagc gagcacggcc cccggtcggc tgaccgcgtt
     3241 ggtggccagc acccccacag cgaacggggt gaaggtcagg tgcgagatca ccacgccgcg
     3301 cagtccggca cccgcggcga gcagcaacgt cacgacgctc aggtgcagcg ccagcgtcgg
     3361 cagcggcgcg aacaggaacg acaccccgat gccgagcgct gtgatcaacg caagcggggt
     3421 cagcgggttg agcctggcca gcacactcac gccgccaccc ccagtgcctc gcgcacggcc
     3481 gcgggcaacg gcagcccggc ccgcgccagc aacggatcgg ccagcaccgc cgcagtcggg
     3541 ccgccggcca gcacctcgcc ctggtggatc aggtaggtgc ggtcggccac ctcgagcacg
     3601 aagcgcaggt cgtgactggt cagcagcacg ccccggcccc gggccgcggc gtcacgaaag
     3661 gcgcgcgccg tccactcgac cccgtcgcgg tcgaggccga aggtgggttc gtcggccaac
     3721 accgcctgac gccggcccag cagcacggtg cccaccgaca gccggcgctg ttcgccgccc
     3781 gagagtcgat gcaccgacaa cccggccgcc ccggtcagcc gaagctcggc caggctcgtc
     3841 tgcacctggt cgtccggggc tccgtcgaag gccagttcgt cgcgcaccgt gctggccaga
     3901 aactgctgct cggggctctg gaagatcaac gccggacgtc cggagggcag ctcgatccgt
     3961 ccggccagcg gcgggcgaat gccggccagc gtctgcagga gggtggtctt gcccgccccg
     4021 ttgcgcccga tcagcgctac cacctcgccc gcgtgcaccg ccacggtggc gcccgtcact
     4081 atcggcgtgc ggtcgtgccc cacggccagc gcccgggtgg ccagcaccgg ttcggcggcc
     4141 ggcggggcgg tgggggccgt gggcggggcc ggacgatggc gccgctccac cggccacggc
     4201 acgtggcagc cggcggcgat caggcgggca ccgtggtcgc gccagacctg ctcggcgggc
     4261 ccgtcggcga tgatcccacc ctcctccagc agcaccaccc ggcccggcag gccggcgccg
     4321 tcggcccact cgtcgagccg atgctcgacc agcagcagcg cgggcggcgc gggggtctcg
     4381 gcgaccgcgg ccaccgccgc gcgcacccgg cgcagcgcct cggggtcgag catcgaggtc
     4441 ggctcgtcga gtagcagcag ggcgggggcg ggtgcggtcg ccccggccag cccgactcgt
     4501 tgcgcctgac cggcagacag ggtgacggtg tcgcggtcgt gccaatcggc ggcgtccacc
     4561 agtagcagcg agcgcagcac ggcggcttcg atctcgccga caccccagca gcggctttcg
     4621 caaccgaaag ccacgtcgtc ggccacccgc ggcaggcaga ccgccgccgc cggatcctgc
     4681 ggcagccagc cgatcggaag cggaggggct gtcaccgggc ggccgagcac gtcgtcgccc
     4741 gacacctcga tcgacccctc gatgcggacg tgcgggccgg gggtgagccc ggcgatcagc
     4801 cgcagcagcg tcgacttgcc cgagccggac gcacccgcca gcagtacctg ctcgccgggt
     4861 tcgatgctca gctcgatgcc gcgcagcagg gggcgtccgt gccgcgggtg cacggtcagc
     4921 ccgcgcaccc gcagcacgtc gctcacgcag gctccgacgc cggcgggtgg tcgtcgagcg
     4981 cgccgatggc gaatgagtcg agcacgcccg ttcgttgcag ggccatgacc agcaaccggg
     5041 ccagcagccc accgagaatg acaccagagg tcacctgcat tcccaaccgc agcagcagca
     5101 ggtcggagcc gaaccagccg gcccgcaacg cgctgaacca gaacaccgca aaggcaccga
     5161 gggcgcctga ggccgcgtac acgccccagc cgaaccggcg gtaccgggtg aggaagaacg
     5221 gcagctcgct gcccaggccc tgcaggaagg cggccaccag cagcagggga ccgaccggcg
     5281 agccgagaaa gacgacctcg atgatgctgg cgatcacttc ggcgacgatg cccgcgccgg
     5341 gccgtcgggt gatggccacc gcgagcgggg ccacgatcag ccaaccgccg agcagcacgt
     5401 gctgggtcag atcgccgaac gggccggcga gcaggctcag cgccacccag gcctgcacca
     5461 gcacccagta gacgaagccg aacacgactc cgagcaccac caccagcatg atgtcgcggg
     5521 tctgccagcg gctcagccaa ccttctcctg acgtgctcat cgcaggttct ccgacgggct
     5581 gttcacgccg atggtgacgt gggtgacggt gtgcggcacg gcgctcccgg cggccagcca
     5641 ggtcgcgaaa accgtccgca gcacgtccga caggtcaccg gccagcgtgg tgacgaagtg
     5701 ctccgcacgc agaaaggtgg cgtcgtgacg ggcccgctcg atcgccgccc acaccggggc
     5761 gaggtggtcg gaaccgtcct cgggggccgg cgccccggcg tccgcgagcg ggtagagcga
     5821 ccattgcgcc tgcaccgcca cgccgctcgg gtcgagccgg acggagctga gcgctggtgg
     5881 cggcaccgcg ccgcacacct cgccggggca gccgcgcgac agcatcaggt gcgccaccac
     5941 atggtgcccg gacgcggcga ccgccgcgat caggtcgcgc aggtactcgg ccagccgctg
     6001 ctcgctgccg cgcacgaagg tggagatctt gtcggtgtcg agctgcaggc cctcgcgatt
     6061 caccgcggcg agggcctggg tgatcagccg gacgtagtcg tcggccatca cggccacggt
     6121 gatccgggca cccacgccgc tggtcgcggc ctgggtgatc gggtctggtg ctgggtggat
     6181 cgcccggccg gtcgaagccg ggcgatcagg ggtgatggtg gtcatcgcag tgtcctcacg
     6241 ttggtgcgga cacgggacgc acgaggttcg ggtgaacctc ggcgtgagcc ttcctgcgcc
     6301 ggcatgatcc ggatcaggtt cgacggtcga aggttcgcaa accttcctct cagcccggtg
     6361 ctaccggact cccgtggtgc cctcacgcta ccgccgcggc cgcgcccggg ggaggcggtg
     6421 ccaccattcg gcgggtcagt gtggcgatcg acccaccgtt cgacgtccga gcacacccca
     6481 ggggatgaac atcggatgac acgcgtggat caagcccacg cacctaccga gttcgggcgc
     6541 gtcactgagc tagcgtgcaa gcacggccgc caccgcgcag ccgtccagga ggtaacgatg
     6601 cgttgtttga ccctgaccgg tcgaacccgc gggccggcac ccggcccacg ccttcccggc
     6661 tgatcaccac atcggccggc gcaggtgcgg cgtgcggccc cgagaacagg agcgctcgtg
     6721 cccacaaccg ccagcacctc tcgccgatcc acccgcagcg ccagcgccac cgacgtgcgc
     6781 acgtacgtga tcgacacctc cgtgctgctc agcgatccgc atgcgttgcg caacttcgat
     6841 gagcacgagg tcatcgtgcc gatcgtggtg atcaccgaac tcgaggccaa acgcgatcac
     6901 cccgaactcg gctacttcgc ccgtaccgcc ctgcgctacc tggacgaact gcgcgtcgcc
     6961 aacggccgcc tcgacctgcc cctgcgggtc aacgaggccg gcggcaccct gcacgtggag
     7021 ttgaaccact ctgaccagtc cctgctgccg gcgggttttc ggctcggcga caacgactcg
     7081 cgcatcctgg ccgtcgcact gaactattcc gacgagggac gcgaggtggt actggtcagc
     7141 aaggacgtgc cgttacgcgt caaggcgtcc gcggtgggcc tgctggccga ggagtatctg
     7201 cacgaactgc ccgccgattc gggttggacg ggcatggccg aggtcgacgt ggcgcagtcc
     7261 agcatcgaaa ccctgtacgc aaccggcgcg gcgatcgtgc ccgaggcggt cgacctgcct
     7321 tgtcacagcg gtgtggtgct gcacgcggcc gggtcgtccg cgctgggccg ggtgacgccc
     7381 gagggcggcg tcaaactggt ccgcagcgac cgggactgct tcggcattca cggacgctcc
     7441 gccgaacagc gggtggcgct cgacctgctg ctcgacccgg cggtcggcat cgtctcgctg
     7501 ggcggacggg cgggcacggg caagtcggcc ctcgcgctgt gcgccgggct cgagtcggtg
     7561 ctggagcgtc agcagcacgc caaggtgatc gtgttccgtc cgctgtacgc ggtgggcggt
     7621 caggagttgg gctacctacc cggtacggag ggcgagaaga tgtcgccctg ggcccaggcc
     7681 gtgttcgaca ccctcggctc ggtgaccaac cgaaacgtga tcgacgaggt gctggcccgc
     7741 gacatgctcg aggtgctgcc gctgacccac attcgcggac ggtccctgca cgacgcgttc
     7801 gtgatcgtgg acgaggcgca atccctggag cgcaacgtgc tgctcaccgt gctctcgcgg
     7861 atcggtcaga actcgcgggt ggtgctcacc cacgacgtgg cccagcgcga caacctgcgg
     7921 gtcggccggc acgacggggt ggtcgccgtg gtcgagaagc tgaaggggca tccgctgttc
     7981 gcccacgtca cgctgacccg ctcggagcgc tcaccgatcg cggccctggt caccgacctg
     8041 ctggaggacg tcggcatcta gcgccgatca acggacgatg cgttggttgg gcttgtcgaa
     8101 ccgtcgattg agttcgtcga aaaccctgct cgtgagtgag ggtctcgagt tcggccagcg
     8161 atagatcacg aggtctcgac aggctcgacc agcgatcgtc cggagccccc acggcatgtc
     8221 ccgcgcgacc cgcccatccc acgcgaaagg gtctggacgc cgagccgcct cgcagggtgt
     8281 gctgagggca tgagaactct cttgcgctgg ttgctcgccg gctccatggt gttcgccgga
     8341 ctcagtcacc tgttctgggc ccgtcgggag tttcaggctc aggtgccgga ctgggtgggt
     8401 gagaccaccg gcctcgatcc ggacgcggtg gtggtcgctt cgggcgcggt cgaggtgatg
     8461 ctcggctccg cactggtggt gctgccgcgc gatcggcgac gggtgggcgc gattctggcg
     8521 gcgttcttcg tcgcggtctt tccgggcaac ctcgcgcagt atctcgatcg ccgcgacggg
     8581 ttcggcctgg ataccgacac caagcggttg gtccggttgt tctttcagcc cctgctggtc
     8641 tgctgggcgc tgtggtgcac caggtccgag cagaagtgac cgcgctgcta gggcgtgtcg
     8701 cataaccgtg ggcgttccgc gaggacgtgc ccagtgcgtc tgatcgcaag gcggaagacg
     8761 agtcgatagt gggtcctctc gccgaggatg acaacgcagc gagcgggcgt actgggtgcg
     8821 ccgcagcgcg gtcagggtta
//
LOCUS       DAOSUG010000054         8753 bp    DNA     linear   ENV 10-JUN-2024
DEFINITION  MAG TPA_asm: Micropruina sp. isolate AMR_MDS_2195
            NODE_4237_length_8753_cov_4.877328, whole genome shotgun sequence.
ACCESSION   DAOSUG010000054 DAOSUG010000000
VERSION     DAOSUG010000054.1
DBLINK      BioProject: PRJNA925477
            BioSample: SAMN32864262
            Sequence Read Archive: SRR4236673
KEYWORDS    WGS; ENV; Metagenome Assembled Genome; MAG; Third Party Data; TPA;
            TPA:assembly.
SOURCE      Micropruina sp. (wastewater metagenome)
  ORGANISM  Micropruina sp.
            Bacteria; Bacillati; Actinomycetota; Actinomycetes;
            Propionibacteriales; Nocardioidaceae; Micropruina.
REFERENCE   1  (bases 1 to 8753)
  AUTHORS   Abdulkadir,N., Saraiva,J.P., Zhang,J., Stolte,S., Gillor,O.,
            Harms,H. and Rocha,U.
  TITLE     Genome-centric analyses of 165 metagenomes show that mobile genetic
            elements are crucial for the transmission of antimicrobial
            resistance genes to pathogens in activated sludge and wastewater
  JOURNAL   Microbiol Spectr 12 (3), e0291823 (2024)
   PUBMED   38289113
REFERENCE   2  (bases 1 to 8753)
  AUTHORS   Abdulkadir,N.
  TITLE     Direct Submission
  JOURNAL   Submitted (24-MAR-2023) Environmental Microbiology, Helmholtz
            Center for Environmental Research -UFZ, Permoserstr 15, Leipzig
            04318, Germany
COMMENT     The annotation was added by the NCBI Prokaryotic Genome Annotation
            Pipeline (PGAP). Information about PGAP can be found here:
            https://www.ncbi.nlm.nih.gov/genome/annotation_prok/
            
            ##Genome-Assembly-Data-START##
            Assembly Method        :: SPAdes v. 3.11.1
            Genome Representation  :: Full
            Expected Final Version :: Yes
            Genome Coverage        :: Not Applicable
            Sequencing Technology  :: Illumina MiSeq
            ##Genome-Assembly-Data-END##
            
            ##Genome-Annotation-Data-START##
            Annotation Provider               :: NCBI
            Annotation Date                   :: 05/04/2023 04:57:10
            Annotation Pipeline               :: NCBI Prokaryotic Genome
                                                 Annotation Pipeline (PGAP)
            Annotation Method                 :: Best-placed reference protein
                                                 set; GeneMarkS-2+
            Annotation Software revision      :: 6.5
            Features Annotated                :: Gene; CDS; rRNA; tRNA; ncRNA
            Genes (total)                     :: 2,776
            CDSs (total)                      :: 2,750
            Genes (coding)                    :: 2,714
            CDSs (with protein)               :: 2,714
            Genes (RNA)                       :: 26
            tRNAs                             :: 24
            ncRNAs                            :: 2
            Pseudo Genes (total)              :: 36
            CDSs (without protein)            :: 36
            Pseudo Genes (ambiguous residues) :: 0 of 36
            Pseudo Genes (frameshifted)       :: 2 of 36
            Pseudo Genes (incomplete)         :: 32 of 36
            Pseudo Genes (internal stop)      :: 2 of 36
            ##Genome-Annotation-Data-END##
FEATURES             Location/Qualifiers
     source          1..8753
                     /organism="Micropruina sp."
                     /mol_type="genomic DNA"
                     /submitter_seqid="NODE_4237_length_8753_cov_4.877328"
                     /isolate="AMR_MDS_2195"
                     /isolation_source="Activated sludge of municipal
                     wastewater treatment plants"
                     /db_xref="taxon:2737536"
                     /environmental_sample
                     /geo_loc_name="Canada"
                     /lat_lon="49.25 N 123.1 W"
                     /collection_date="2015-06-16"
                     /metagenome_source="wastewater metagenome"
                     /note="metagenomic"
     gene            139..1017
                     /locus_tag="PLF56_02970"
     CDS             139..1017
                     /locus_tag="PLF56_02970"
                     /inference="COORDINATES: protein motif:HMM:NF024621.2"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="DUF4031 domain-containing protein"
                     /protein_id="HOQ52556.1"
                     /translation="MAVLVDSPRWPAHGTQFGHLVSDTSLDELHAFAHAAGVPPRAFD
                     HDHYDVPAARYPDLLALGAVQVGSTELLRRLVAAGLRVRPAQRTPRRAAARVAAESAW
                     PLPETDGLRDELLERWVEPQRVYHDVRHLAQCLDALRQLQPLPAPRPVVLAVWFHDAV
                     YQGDPGADERASAQLADQRLAGLVPPDEVREVVRLVLGTIAHDPAPGDHNAALLMDAD
                     LSVLGQIRGRYHVYARDVREEYTRYDDESYRRGRLTVLDDLLDRTQLFHTEPGRALWD
                     EAARRNLAEERTRLTG"
     gene            1246..1941
                     /locus_tag="PLF56_02975"
     CDS             1246..1941
                     /locus_tag="PLF56_02975"
                     /inference="COORDINATES: protein motif:HMM:NF040120.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="DUF6498-containing protein"
                     /protein_id="HOQ52557.1"
                     /translation="MTNRLRLMDVGWVLVTNLLLLAGVLAWGWPAGNVFVLFWIENVI
                     LGAATAVKIATADGVDPASASTTPRWARTLFFVFHYGIFALVHGVFSVIIAFGIGFSF
                     SLWALGLPAALIALRYGVELATGWFLGDARRTVTPGQAFASPYPRLIVLHVATILGFF
                     VVMSSTFRTTAGGRAAGPLGPLLNWLDAHGHPVSSGLLVVSILLVIKTIADLLVLRSR
                     AKGTPLKFSYGTS"
     gene            2353..3189
                     /locus_tag="PLF56_02980"
     CDS             2353..3189
                     /locus_tag="PLF56_02980"
                     /inference="COORDINATES: protein motif:HMM:NF014600.2"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="M15 family metallopeptidase"
                     /protein_id="HOQ52558.1"
                     /translation="MSGPQLRGLSPATFTRTLTAAALTVALVGCAAAAPGAGPSSEPA
                     GSSSPTLSARASATATPSATAGSPTASTSTPAASRSATPSTTADAPKDTRTAAKRNRA
                     FMVNGIPVISKTHPINADYRRSSVTSPNGLTTQAQTALTRMIAAARAAGVTIRVRSGY
                     RSYALQAQILASKIKEYGSETKARRYNAAPGRSEHQTGLAVDLWDGVTWGVGVRSTKV
                     GKWLWANAWRYGFILRYPDGKEKITGYAFEPWHYRWIGTADAKHFGANNTLTLEEYLG
                     LA"
     gene            3234..3992
                     /locus_tag="PLF56_02985"
     CDS             3234..3992
                     /locus_tag="PLF56_02985"
                     /inference="COORDINATES: protein motif:HMM:NF004826.0"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_function: GO:0016491 - oxidoreductase activity
                     [Evidence IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="oxidoreductase"
                     /protein_id="HOQ52559.1"
                     /translation="MRGARSGAARRTERLEKLSTEGIRAVAMDVTDEASMTAGIAKII
                     AEAGRIDVLVNNAGYGSYGALEDVDIEEGRRQFEVNAFGAMRLTQLVVPHMRAQRSGK
                     IVNITSMGGKLHTPLGGWYHGTKFALEALSDCLRMEVAPFGIDVVVIEPGGIATEWGA
                     IAADHVEATSGTGPYSAQAKAVANSLRSEANANRSSPPSVIADAIAKAVTARRPRTRY
                     GVGFGAKPLIAARGLLSDRQYDALIARATGLPRS"
     gene            complement(4145..4996)
                     /locus_tag="PLF56_02990"
     CDS             complement(4145..4996)
                     /locus_tag="PLF56_02990"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_013864923.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="aldo/keto reductase"
                     /protein_id="HOQ52560.1"
                     /translation="MRTVTLNNGVEMPILGFGVFQIADAAECERAVSDALAAGYRALD
                     TAASYLNEEAVGRAIQSSGIPRDELFVTTKLWIQGAGEGKTRAAFERSLQRLGLDHVD
                     LYLIHQPYGDVYGAWRDMEKINAEGLARAIGVSNFHPDRLVDLIAHNDIVPAVDQVET
                     HPFHQRIADQELMAAEGVQIESWGPFAEGKNGLFTNPILCAVAEAHGKSVGQVVLHWL
                     TQRGVVVIPKTVRPKRMAENLDVFDLKLTDEEMASIASLDTGTSQFFDHRDPAMVKWL
                     SGRALDV"
     gene            5126..5992
                     /locus_tag="PLF56_02995"
     CDS             5126..5992
                     /locus_tag="PLF56_02995"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_017935349.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_function: GO:0003677 - DNA binding [Evidence IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="helix-turn-helix transcriptional regulator"
                     /protein_id="HOQ52561.1"
                     /translation="MDNRREINEFLTSRRARVRPQDSGLPLLDDRRRVPGLKREEVAL
                     LAGVSVDYYARLERGQLGGVSPSVLDAIADALKLDEAERGHLRDLAQAAAVPERTLRS
                     RTPKALPLRPSIRTLLDGMTGVPAYVRNGRAEILHANDLCLALYDGILQRDAPFNLAR
                     FVFLDGRASGFFPDWDEVADGTVGALRAEAGRNPLDRPLTDLVGELTTRSHPFATRWA
                     RHDVRLHRTATKRLHNSVVGDLELTGDALELTGDNLTIIAYTAEPGSASADKLALLAS
                     WQHTDAPARSSR"
     gene            complement(6015..>6377)
                     /locus_tag="PLF56_03000"
     CDS             complement(6015..>6377)
                     /locus_tag="PLF56_03000"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_013863343.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="aldo/keto reductase"
                     /protein_id="HOQ52562.1"
                     /translation="TSTTFADGDFRGQTPRFQGEALQANLAVVEALKRIAAEVGATPA
                     QLALVWLLNQQPWIVPIPGTKRVERLEENIGASQVELTDAQLADLRAAADQVQILGAR
                     YPEAQDRMTNQEAPLPTR"
     assembly_gap    6378..6477
                     /estimated_length=100
                     /gap_type="within scaffold"
                     /linkage_evidence="paired-ends"
     gene            complement(<6478..7005)
                     /locus_tag="PLF56_03005"
     CDS             complement(<6478..7005)
                     /locus_tag="PLF56_03005"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_013863343.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="aldo/keto reductase"
                     /protein_id="HOQ52563.1"
                     /translation="MKYRNLGGNLDVSEIGLGCMSLTGAYGNTGGPDKAEGIALIRHA
                     VELGVTFFDTAEVYGPFRNEEIVGEALEPVRDQVKIATKFGFAFAENGVEPGGLCSRP
                     ESIRAAVDNSLRRLRTDHIDLYYQHRVDTSVPIEDVAGTVKELIQAGKVLNFGLSEAA
                     AATVRRAHAVQPVAAV"
     gene            7241..7558
                     /locus_tag="PLF56_03010"
     CDS             7241..7558
                     /locus_tag="PLF56_03010"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_020576291.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_function: GO:0051920 - peroxiredoxin activity [Evidence
                     IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="carboxymuconolactone decarboxylase family
                     protein"
                     /protein_id="HOQ52564.1"
                     /translation="MAEQSRAQQLVGDIAPKLAELTDDVLFGDVWARPELSRRDRSLI
                     TVAALTALGRTDQLRSHLNLALDNGVTRDELSEVATHLAFYAGWPAGMTAATTLTQVL
                     DAH"
     gene            complement(7856..8356)
                     /locus_tag="PLF56_03015"
     CDS             complement(7856..8356)
                     /locus_tag="PLF56_03015"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_008357571.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="DUF6326 family protein"
                     /protein_id="HOQ52565.1"
                     /translation="MTIRTTPANLLDTPAIPVQARLAAAWTSFMFLYVYVDILNFYKP
                     GVVDGIRNGLIWTFDISSTLLTIFLVSVSIPALMVVLSMTLPALWNRATNLVIASLLI
                     PYSVFNAAGATWEWAGFYGISIGVEVLLLAFILRSAWVWPRTGAAHLATARTGPGRQV
                     SVPDLP"
     gene            complement(8454..>8753)
                     /locus_tag="PLF56_03020"
     CDS             complement(8454..>8753)
                     /locus_tag="PLF56_03020"
                     /inference="COORDINATES: protein motif:HMM:NF024989.2"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="zinc-binding dehydrogenase"
                     /protein_id="HOQ52566.1"
                     /translation="VRRALTPVGTLIPNSGRGGRWLGPIPRMLKASLLSGFTRQRLKP
                     FTSIGRRKDLLTLAELLRTGQVEPLIDRTYVLDDTADALGYIADGHTRGKVVITL"
BASE COUNT         1332 a         3074 c         2890 g         1357 t
ORIGIN      
        1 ccatcgccat tccatgggtc ggctcgcaca tcgagaacgg gcagttcgtc ggctacgacg
       61 cgctggacca gagcatcgat gcggtctacg actcgctggt cgagctgcag gcagcgtccg
      121 cgcgcgagcg cgactgacgt ggcggttctg gtcgattcgc cccgttggcc cgcccacggg
      181 acgcagttcg gccacctggt ctctgacacc tcgctggatg agctgcatgc cttcgcccac
      241 gcggccggcg tgccgccgcg ggccttcgat catgaccact acgacgtgcc cgcggctcgc
      301 taccccgacc tgctcgcgct cggtgcggtt caggtcggtt cgacggagct actgaggcgg
      361 ctggtcgccg ccgggctgcg agtgcgtccg gcgcagcgca ccccgcgccg cgctgccgca
      421 cgggtggcgg ccgaatccgc ctggccgctg cccgaaacgg acgggctgcg cgacgagttg
      481 ctcgagcgct gggtcgagcc ccagcgggtc taccacgacg tccgtcacct ggcgcagtgc
      541 ctggacgcgc tgcggcaact ccagccgcta cccgcacctc gccccgtcgt gctcgcggtg
      601 tggtttcacg acgcggtcta ccagggcgac ccgggggcgg acgaacgcgc ttcggcccag
      661 ttggcggatc agcgactggc cggcctggtg ccgccggacg aggtgcgcga ggtcgtccgg
      721 ctggtgctgg gcaccatcgc gcacgatccg gcaccgggcg accacaacgc cgcgctgctg
      781 atggacgccg acctgtcggt gctcggccag atccgcggcc gctaccacgt gtacgcccgt
      841 gacgtgcgcg aggagtacac ccgctatgac gacgagtcct accgtcgtgg acggctcacc
      901 gtgctggacg acctgctgga ccggacgcag ctgttccaca ccgagcctgg acgcgccttg
      961 tgggacgaag ccgcccggcg caatctcgcc gaagaacgaa cgagactcac cggctgaccc
     1021 cgcgagcagg accgcgcccg tacgtcgcca cggcgttgtc gcggacgcag gtgcgccgcg
     1081 acaacaacag caacgccgca gtggccgttc gaggcaggtg cgcggctcca ttcgcgcggg
     1141 taccgacagc cctcgggtcg gccaccgcgg gcgggcccgc tccgagccga ctcgatctgc
     1201 gaccccactc gcggtagcag accccgtccg ccctatcgtc ggtgcatgac caatcgtctt
     1261 cggctgatgg acgtgggctg ggtgctggtc accaacctgc ttctgctggc cggtgtgctc
     1321 gcgtgggggt ggcccgccgg caacgtgttc gtgctgttct ggatcgagaa cgtgattctg
     1381 ggcgcggcga cggccgtcaa gatcgccacg gcagacggcg tcgaccccgc ctcggccagc
     1441 acgactccgc gctgggcccg caccctgttc ttcgtctttc actacggcat attcgccctg
     1501 gtgcacggtg ttttctcggt gatcatcgcg ttcggcatcg ggttctcgtt cagtctctgg
     1561 gcactgggac taccggccgc cttgatcgca ctgcgctacg gcgtcgagtt ggcgaccggc
     1621 tggtttctgg gtgatgcgcg gcgcaccgtc acgcccggcc aggcgttcgc atcgccgtac
     1681 ccgcgcctga tcgtgctgca tgtggcgacc attctcggct tcttcgtggt catgtcgtcg
     1741 accttccgaa ccaccgcggg cggtcgcgca gcaggcccgc tcggcccgct gctgaactgg
     1801 ctggacgccc acggccaccc ggtcagcagc ggcttgctgg tggtgagcat cctgctggtg
     1861 atcaagacca tcgccgatct gctcgtgctg cgctcgcgcg ccaagggcac gccgctgaag
     1921 ttcagctacg gcacgtcctg agccggcgat ccctggtcgc cggatgtttc tccccgaggc
     1981 ctgtggaaag ggctagctag tgacgcggcg ccgggcgatc cctgaaaagc ctgcaccagt
     2041 aacccgaccc ctgcctcggc acggcgggag gtaccgtact ggtgcgggct tttcaggcgc
     2101 ctgcctgggt gcccctcgcc gtcggtcggt ccctggtccg ccccgcgcgc agcctgagaa
     2161 tcgaagaagc agatccgcca ccttgtccgg cgcgcagtcg gatggttggt tcggggacgg
     2221 ttcccaacac ggtgcataga aatccccgga caaccgacct ggacgcccgc agcagccgcc
     2281 cggacgtctc agcgctatcc ggcccccgct acggatccct gcgctacccg gcccttgggc
     2341 tatcgtgcat cggtgtcagg tccgcagctc cgggggttga gccccgcgac tttcacccgc
     2401 actctcaccg ccgcggcgct cacggttgcc ttggtgggct gcgccgcagc ggcacccggg
     2461 gccggtccct ccagtgagcc cgccggctcg tccagcccga ccctcagcgc ccgggccagc
     2521 gccacggcga cgccgtccgc caccgccggt tcgcccaccg cttcgacctc gacccccgct
     2581 gcctcacgct cggcaacccc aagcaccacg gccgacgctc cgaaggacac ccgcaccgcc
     2641 gccaagcgca accgggcgtt catggtcaac ggcattccgg tgatctcgaa gacgcacccg
     2701 atcaacgcgg actaccggcg cagcagcgtc acttcgccca acggtctgac cacgcaggcc
     2761 cagacggcgc tgaccaggat gatcgccgcc gctcgagccg ccggggtcac gatccgggtg
     2821 cgcagcggct accgcagcta tgcgctgcag gcgcagattc tggccagcaa gatcaaggag
     2881 tacggcagcg agaccaaggc gcggcgctac aacgcggcac ctggacgcag cgaacaccaa
     2941 accgggctgg ccgtcgacct gtgggacggg gtgacctggg gcgtcggggt gcgcagcacg
     3001 aaggtcggca agtggctgtg ggcgaacgcc tggcgctacg gcttcatcct gcgctacccc
     3061 gacggcaagg agaagatcac tgggtacgcc ttcgagccct ggcactaccg ctggattggc
     3121 accgccgatg cgaaacactt cggcgccaac aacaccctca cgctcgagga gtacctgggc
     3181 ctggcatagg gcctgacaga attcgatggc cgagtcagtc gtccgctgac catttgcgtg
     3241 gggccaggag tggggcggcc cgccgaaccg aacgacttga gaagctctcc accgaaggga
     3301 ttcgggcggt cgcgatggac gtcaccgacg aagcatcgat gaccgccggc atagcgaaga
     3361 tcatcgccga ggccggccgg atcgacgtgc tggtcaacaa cgccggttac ggctcttacg
     3421 gcgccctgga ggacgtcgac atcgaggagg gtcgccgcca gttcgaggtc aacgcgttcg
     3481 gcgcgatgcg gctgacccaa ctcgtggtgc cgcacatgcg cgcacaacga tcgggcaaga
     3541 tcgtgaacat cacctcgatg ggcggcaagc tgcacactcc gctcggcggc tggtaccacg
     3601 gcaccaagtt cgccctcgaa gcgctcagcg actgcctgcg catggaggtc gcccccttcg
     3661 ggatcgacgt ggtggtcatc gagcccggcg gcatcgccac cgagtggggc gccatcgccg
     3721 ccgatcacgt cgaagcgacc tccgggaccg gaccgtactc cgcccaggcc aaggcggtgg
     3781 ccaactcgct gcggtcggag gccaatgcca accgcagctc tccgccctct gtgatcgccg
     3841 acgccatcgc caaggccgtg accgcccggc gacccaggac ccgctacggg gtcggtttcg
     3901 gtgccaagcc gctcatcgcc gcccgcggcc tgctgtccga ccgccagtac gacgccctca
     3961 tcgcccgcgc taccggactc ccccgcagct gacgggcagg gacctctgac tcccggcgcc
     4021 catagagcgg aggctgccga ccgcgacctc gccgtcgccg ctgcggtcga cggcgcaatg
     4081 agcgaccccg gccaggctgc cgaggtttcg tgacttcaag gtcgattcgc tcaggtctcg
     4141 ccggtcagac gtccagggca cggccggaca accacttgac catcgcgggg tcgcggtggt
     4201 cgaagaactg gctggtgccg gtgtcgaggc tggcgattga cgccatttcc tcatcggtga
     4261 gctttaggtc gaacacgtcc aggttctctg ccatccgctt ggggcggacg gtcttgggga
     4321 tcacgaccac gccccgctgg gtcagccagt gcagcaccac ctgtccaaca ctctttccgt
     4381 gggcctcggc gacggcgcac aggatcgggt tggtgaacag gccgttcttg ccctctgcga
     4441 acggtcccca ggactcgatc tggacgccct cggccgccat cagctcctgg tcggcgatgc
     4501 gctggtggaa ggggtgggtt tcgacctggt cgactgccgg gacgatgtcg ttgtgggcga
     4561 tcaggtcgac cagccggtcg gggtggaagt tcgagacgcc gatcgcccgg gcaaggcctt
     4621 cggcgttgat cttctccatg tcgcgccagg caccgtagac gtcgccgtag ggctggtgga
     4681 tgaggtacag gtcgacgtgg tcgaggccga ggcgctgcag ggagcgttcg aacgcggcgc
     4741 gggtcttgcc ctcgccggcg ccctggatcc acaacttcgt ggtgacgaac agctcgtcgc
     4801 gcgggatgcc ggagctctgg atggcgcgtc cgacggcttc ctcgttgagg tatgacgctg
     4861 ccgtgtcgag cgcccggtac ccggcggcaa gcgcgtcgct caccgcgcgc tcgcactcgg
     4921 ccgcatcggc gatctggaag acgccgaagc cgaggatcgg catctcgacg ccgttgttca
     4981 gggtcacggt acgcatgggt ggttccttcc agttcgatcg gtacgacgcc agtccactcc
     5041 tgtgccctcg cgcgcggaag gccctggcgt accgcgcact gccagtacct gttctgcggc
     5101 aacgggccgg cggtagcgtc accgggtgga caaccgccgc gaaatcaacg agttcttgac
     5161 cagccgcagg gcacgcgtcc ggccgcagga ctccgggctg ccgctcctcg acgaccgccg
     5221 tcgggtgccg ggcctcaagc gtgaagaggt cgcccttctc gccggggtca gcgtcgacta
     5281 ctacgcccgc ctcgaacgcg gtcagctcgg cggcgtctcc ccgtcagttc tggacgcgat
     5341 cgccgacgcg ctcaagctcg acgaagccga acgcggacac ctccgcgacc tcgcgcaggc
     5401 tgcggccgtg cccgaacgaa ccctccgcag ccggacgccc aaggcgctgc cgctgcggcc
     5461 gagcatccgc acgctcctcg acggaatgac cggcgtcccc gcctacgtcc gcaacggacg
     5521 cgccgagatc ctccacgcca acgacctgtg cctcgcgctc tacgacggca tcctccagcg
     5581 cgacgcgccg ttcaacctcg cccgcttcgt gttcctcgac gggcgcgcct ccggcttctt
     5641 cccggactgg gacgaagtcg ccgacggcac cgtcggcgcc ctccgggccg aagccggacg
     5701 caacccgctc gacaggccgc tcactgacct ggtcggcgaa ctcaccactc gcagccaccc
     5761 gttcgccacc cgatgggccc gccatgacgt ccggctccat cgcaccgcga ccaagcgatt
     5821 gcacaactcc gtcgtcggag atctcgaact caccggcgat gcgctcgaac tcaccggcga
     5881 caacctcacg atcatcgcct acaccgccga gcccggcagc gcgtccgcag acaagctggc
     5941 cctgctcgcc agctggcagc acaccgacgc tccagccagg agcagcaggt aaggagccgc
     6001 gcggcctgga ccgcctacct ggtgggcagc ggcgcttcct ggttggtcat ccggtcctgc
     6061 gcctcggggt agcgagcgcc gaggatctgt acctggtcgg ctgcggcgcg caggtcggcg
     6121 agctgggcgt cggtgagttc gacctgcgat gctccgatat tctcctcgag ccgctcgacc
     6181 cgcttagtgc ccgggatcgg cacgatccac ggctgctggt tcaacagcca caccagcgcc
     6241 agctgcgcag gggtcgcccc tacctcggcc gcgattcgct tcagcgcctc gaccaccgcc
     6301 aagttcgcct gcagggcctc cccctggaac cgcggcgtct ggccccggaa gtcaccgtcg
     6361 gcaaacgtgg tggacgtnnn nnnnnnnnnn nnnnnnnnnn nnnnnnnnnn nnnnnnnnnn
     6421 nnnnnnnnnn nnnnnnnnnn nnnnnnnnnn nnnnnnnnnn nnnnnnnnnn nnnnnnncac
     6481 tgctgcgacc ggctggacgg cgtgcgcccg gcggacggtg gccgccgccg cctccgaaag
     6541 cccgaagttg agcaccttgc ctgcctggat cagctccttc accgtccccg cgacgtcctc
     6601 gatcgggacg gaggtgtcga cgcggtgctg gtagtacagg tcgatgtggt cggtgcgtag
     6661 ccggcgcagc gagttgtcga ccgctgcccg gatgctctcc ggccggctgc acaacccgcc
     6721 cggctcgacc ccgttctcag cgaaagcgaa accgaacttc gtggcgatct tcacctggtc
     6781 gcgcaccggc tccaacgcct cgccgacgat ctcctcgttg cggaacgggc catacacctc
     6841 ggcggtgtcg aagaacgtga cgcccaactc gacagcgtgg cggatcaggg cgatcccctc
     6901 cgccttgtcc ggcccgccgg tgttgccgta ggcaccggtc aggctcatgc agcccaaacc
     6961 gatctccgaa acgtccaggt ttccgccaag gttgcggtac ttcatcacag ggagtccttc
     7021 cggtcgtgca ccaatgatgt gggtgcggcg gatgctcgca tcggatcacc gccacgcctc
     7081 tcccgccact ccatcacaca tggcctgggt gcaggaggcc ctgtcagtac ctcctcgggc
     7141 ccgatcgggc gtactggcag tgcctgcctc cgtcagctct gccgggctag cgtcaatggc
     7201 acggagcgat ccgctccgct ggagcaacga gaggacccca atggcagagc agagccgcgc
     7261 ccaacagctg gtgggcgaca tcgcgccgaa actggccgaa ctcaccgacg acgtgctgtt
     7321 cggcgacgtc tgggcacgac cggaactctc ccgacgcgac cggagcctca tcacggtggc
     7381 agccctgaca gcgctcggac gcaccgacca actccgctcc cacctcaacc tcgccctgga
     7441 caacggcgtc acccgcgacg aactctccga ggtcgccacc cacctggcgt tctacgccgg
     7501 atggccggcc ggcatgaccg ccgcaaccac cctcacgcag gtactggacg cccactgacg
     7561 cccgcttgga ctcgcgcggc cgcgatcatg ccgcccgctc tcgccggcac tggcgggccc
     7621 gaacccggtc gcccggatcg cgcgcgcagg aatcccgccg acgacggtgt ccgccggcac
     7681 gtccttggtg acgacgcccg gcacgacggt gaccggagcc cccagccaga cgttgcgtcc
     7741 gatcgtgatc ggagccggga tcatgtcagc gcggcggtcg gggtcgaccg cgtggttgag
     7801 agggcgctgg aggccttcgc gggaacagct gagcgggcag ccgggcggga cccggtcagg
     7861 ggagatcggg cacggacacc tggcgtccgg gtccggtcct cgcggtggcg aggtgtgcgg
     7921 caccggttcg gggccagacc cacgcggagc gcaggatgaa ggccaggagc agcacctcga
     7981 ctccgatgga gatgccgtag aagccggccc actcccaggt cgctcctgcc gcgttgaaca
     8041 ccgagtaggg gatcagcagc gacgcgatga cgaggtttgt ggcgcggttc cacagggcgg
     8101 gcagtgtcat ggagagcacg accatcagcg ccgggatcga cacggacacg aggaagatcg
     8161 tcaacagcgt cgagctgatg tcgaaggtcc agatgagtcc gttccggatg ccgtcgacga
     8221 cgccgggctt gtagaagttg aggatgtcga cgtagacgta caggaacatg aagctggtcc
     8281 atgcggcggc gagcctggcc tggacgggga tcgccggggt gtcgagcagg ttcgcaggtg
     8341 tcgttcggat ggtcatcggt tcttccttct tcagaagcgg cccgtccgcc gggtaggagg
     8401 cgggtccggg tggggatacg cggggatggg gtccttctcg tcgatccggt ggctcagagg
     8461 gtgatcacca ccttccctcg cgtgtggccg tccgcgatgt agccgagggc gtcggctgtg
     8521 tcatcgagga cgtaggtgcg gtcgatgaga ggttcgacct gcccggtcct gaggagttcg
     8581 gccagggtca gcaggtcctt tcgcctcccg atggacgtga agggcttcag ccgctgacgg
     8641 gtgaagccgg atagcaggct ggccttgagc atccgcggga tggggccgag ccagcgtccg
     8701 ccgcgtccgc tgttggggat gagggtgccg accggcgtca gcgcccggcg gac
//
LOCUS       DAOSUG010000055         8681 bp    DNA     linear   ENV 10-JUN-2024
DEFINITION  MAG TPA_asm: Micropruina sp. isolate AMR_MDS_2195
            NODE_4270_length_8681_cov_5.455483, whole genome shotgun sequence.
ACCESSION   DAOSUG010000055 DAOSUG010000000
VERSION     DAOSUG010000055.1
DBLINK      BioProject: PRJNA925477
            BioSample: SAMN32864262
            Sequence Read Archive: SRR4236673
KEYWORDS    WGS; ENV; Metagenome Assembled Genome; MAG; Third Party Data; TPA;
            TPA:assembly.
SOURCE      Micropruina sp. (wastewater metagenome)
  ORGANISM  Micropruina sp.
            Bacteria; Bacillati; Actinomycetota; Actinomycetes;
            Propionibacteriales; Nocardioidaceae; Micropruina.
REFERENCE   1  (bases 1 to 8681)
  AUTHORS   Abdulkadir,N., Saraiva,J.P., Zhang,J., Stolte,S., Gillor,O.,
            Harms,H. and Rocha,U.
  TITLE     Genome-centric analyses of 165 metagenomes show that mobile genetic
            elements are crucial for the transmission of antimicrobial
            resistance genes to pathogens in activated sludge and wastewater
  JOURNAL   Microbiol Spectr 12 (3), e0291823 (2024)
   PUBMED   38289113
REFERENCE   2  (bases 1 to 8681)
  AUTHORS   Abdulkadir,N.
  TITLE     Direct Submission
  JOURNAL   Submitted (24-MAR-2023) Environmental Microbiology, Helmholtz
            Center for Environmental Research -UFZ, Permoserstr 15, Leipzig
            04318, Germany
COMMENT     The annotation was added by the NCBI Prokaryotic Genome Annotation
            Pipeline (PGAP). Information about PGAP can be found here:
            https://www.ncbi.nlm.nih.gov/genome/annotation_prok/
            
            ##Genome-Assembly-Data-START##
            Assembly Method        :: SPAdes v. 3.11.1
            Genome Representation  :: Full
            Expected Final Version :: Yes
            Genome Coverage        :: Not Applicable
            Sequencing Technology  :: Illumina MiSeq
            ##Genome-Assembly-Data-END##
            
            ##Genome-Annotation-Data-START##
            Annotation Provider               :: NCBI
            Annotation Date                   :: 05/04/2023 04:57:10
            Annotation Pipeline               :: NCBI Prokaryotic Genome
                                                 Annotation Pipeline (PGAP)
            Annotation Method                 :: Best-placed reference protein
                                                 set; GeneMarkS-2+
            Annotation Software revision      :: 6.5
            Features Annotated                :: Gene; CDS; rRNA; tRNA; ncRNA
            Genes (total)                     :: 2,776
            CDSs (total)                      :: 2,750
            Genes (coding)                    :: 2,714
            CDSs (with protein)               :: 2,714
            Genes (RNA)                       :: 26
            tRNAs                             :: 24
            ncRNAs                            :: 2
            Pseudo Genes (total)              :: 36
            CDSs (without protein)            :: 36
            Pseudo Genes (ambiguous residues) :: 0 of 36
            Pseudo Genes (frameshifted)       :: 2 of 36
            Pseudo Genes (incomplete)         :: 32 of 36
            Pseudo Genes (internal stop)      :: 2 of 36
            ##Genome-Annotation-Data-END##
FEATURES             Location/Qualifiers
     source          1..8681
                     /organism="Micropruina sp."
                     /mol_type="genomic DNA"
                     /submitter_seqid="NODE_4270_length_8681_cov_5.455483"
                     /isolate="AMR_MDS_2195"
                     /isolation_source="Activated sludge of municipal
                     wastewater treatment plants"
                     /db_xref="taxon:2737536"
                     /environmental_sample
                     /geo_loc_name="Canada"
                     /lat_lon="49.25 N 123.1 W"
                     /collection_date="2015-06-16"
                     /metagenome_source="wastewater metagenome"
                     /note="metagenomic"
     gene            complement(115..669)
                     /locus_tag="PLF56_03025"
     CDS             complement(115..669)
                     /locus_tag="PLF56_03025"
                     /inference="COORDINATES: protein motif:HMM:NF013183.2"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_function: GO:0030151 - molybdenum ion binding [Evidence
                     IEA];
                     GO_function: GO:0046872 - metal ion binding [Evidence
                     IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="MogA/MoaB family molybdenum cofactor
                     biosynthesis protein"
                     /protein_id="HOQ52567.1"
                     /translation="MAERTRTTDVAAAAEAALRSAAGLRATVIVVSDEVASGADTDRG
                     GPVAVDLLAAHGVPADRCMVPDDPGRLRAAIGAAIDSGARVVLACGGTGIGPRDWTSD
                     VVRALIDVEIPGIGEEIRRRGSAHTRLALVSREVAGVVLRDEAPSVLLLAAPGSRGGI
                     RDTLGVVGDLIGYILEQLDGAGHV"
     gene            complement(673..2073)
                     /locus_tag="PLF56_03030"
     CDS             complement(673..2073)
                     /locus_tag="PLF56_03030"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_014845875.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_function: GO:0022857 - transmembrane transporter
                     activity [Evidence IEA];
                     GO_process: GO:0055085 - transmembrane transport [Evidence
                     IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="MFS transporter"
                     /protein_id="HOQ52568.1"
                     /translation="MSTMMEKLQGGPEWLRAWDPENDETWNSKLAWQTLWITTFSLTM
                     CFVTWFLPSAIVPKLNAIGYSFTKEQLYWLAAVPGLSGGLMRLLWMVLPPIMGTRKMV
                     AATTLLLFFPVLGWGVRSMSPTAPYWELLVLAFLAGIGGGAFSGFMPSTSYFFPRSKQ
                     GTALGLQAGIGNFGVSLVQLLTPWLIGTGMLWWMGQSQMLKTAGKPDREVWYQAASFV
                     WLPFILIGTWIAWQYLKSVPIKANIKQQFDIFSNQDTWWMTILYIMTFGTFSGLSAQF
                     GLLMINLYGSGNAGIVQGAGSTAQILVAGYQVPDPATYVFLGPLVGAGARVLFSPLTD
                     RMGGAIWTLISGIGLAASIAWTVPSLTPDLTSAATLQAGFNHFLWGMLLIFLFSGIGN
                     ASTFKQMPMIFERRQAGGVIGWTAAIAAFGPFLFGVGLTIMAPATFYWIGVAWAIMCV
                     GITWWRYARPGAPKKS"
     gene            complement(2070..3443)
                     /locus_tag="PLF56_03035"
     CDS             complement(2070..3443)
                     /locus_tag="PLF56_03035"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_014845876.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_function: GO:0022857 - transmembrane transporter
                     activity [Evidence IEA];
                     GO_process: GO:0055085 - transmembrane transport [Evidence
                     IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="nitrate/nitrite transporter"
                     /protein_id="HOQ52569.1"
                     /translation="MSTPTPPAEDVVPPVHDTGRGANRVLAMSTIDFTVMFAVWLMFG
                     ILGIEIQKELLLDDVQLSWITALAVLNGSMWRLPAGIIADRIGGRKVTIFMLAATAVP
                     AYLVSTAHSYGMLLLLAFLVGFAGNLFSVGVAWNAAWFGRERQGFALGLFGAGNVGAS
                     ITKFIGPPLIAGTAGMTFAFGVQGGWRLVPVIYSVLLLALAVITFLVVPRHDRMAGPG
                     KPIREMLVPLKHLRVWRFSLYYVAVFGAYVALSAWLPKYYVDNFKVDLYTAALLTALF
                     IFPASLLRPVGGWVSDKLGARKVMYVTFAVMLVTTGILMMPNGHIVIAHADGTQTEHL
                     GYGINVVWFTVLVFLLGCSMGIGKAAVYKHIPEYFPDNVGSVGGLVGMLGGLGGFILP
                     PLFAYTKAWTGFPTSTFFVLFVLTAICAVWMHWTIVGMLHEQSPELADHFDTEHIDTR
                     SRQEVGA"
     gene            complement(3440..4204)
                     /gene="narI"
                     /locus_tag="PLF56_03040"
     CDS             complement(3440..4204)
                     /gene="narI"
                     /locus_tag="PLF56_03040"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_002549346.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_component: GO:0009325 - nitrate reductase complex
                     [Evidence IEA];
                     GO_function: GO:0008940 - nitrate reductase activity
                     [Evidence IEA];
                     GO_process: GO:0019645 - anaerobic electron transport
                     chain [Evidence IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="respiratory nitrate reductase subunit gamma"
                     /protein_id="HOQ52570.1"
                     /translation="MNALDIMLWGVLPYLVALVLIAGTIWRYKYDQFGWTTRSSQLYE
                     SRLLRIASPMFHYGLFVVLMGHLMGLLIPKAWTDLVVSQEAYHAVALGAGTVAGVATL
                     AGIALLIYRRRTTGPVFLATTRNDKLMYVILVAAIVLGMLATLTGDHTPTGEHHNYRE
                     TVSVWFRSLLVFQPNVEAMAESTWQFKVHILMGFALIALIPFSRLVHAFTVPLHYLFR
                     PYVVYRSRDDAPAAPSHGRVRGWDPIGTKDRERTKP"
     gene            complement(4206..5033)
                     /gene="narJ"
                     /locus_tag="PLF56_03045"
     CDS             complement(4206..5033)
                     /gene="narJ"
                     /locus_tag="PLF56_03045"
                     /inference="COORDINATES: protein motif:HMM:TIGR00684.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_function: GO:0051082 - unfolded protein binding
                     [Evidence IEA];
                     GO_process: GO:0051131 - chaperone-mediated protein
                     complex assembly [Evidence IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="nitrate reductase molybdenum cofactor assembly
                     chaperone"
                     /protein_id="HOQ52571.1"
                     /translation="MRRLTEWLRRDPGVHRDDGRGDDDPTRPDTPSRPDIPANAGISG
                     RTRGDALVYQAAALVLAYPEAALLDRLDVIESALSGTTDEPLFAPVLAHLRGDSLREL
                     QSFHVQEFDLSRRHALHLSYWTDGDTRRRGNVLAEIKQVYRDSGLVVDTGGELPDYLP
                     MVLEFAVDDPERGFALLERFRASLELIRLELAADHLPHAGVLTAICTRLPGESPRNRA
                     EVQARFGQTQPVELVGLDLVAVNGVPAFAGMTSPTQTGTADAVIPGRDPGSSPKGRS"
     gene            complement(5030..6664)
                     /gene="narH"
                     /locus_tag="PLF56_03050"
     CDS             complement(5030..6664)
                     /gene="narH"
                     /locus_tag="PLF56_03050"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_012921752.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_component: GO:0009325 - nitrate reductase complex
                     [Evidence IEA];
                     GO_function: GO:0008940 - nitrate reductase activity
                     [Evidence IEA];
                     GO_process: GO:0009061 - anaerobic respiration [Evidence
                     IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="nitrate reductase subunit beta"
                     /protein_id="HOQ52572.1"
                     /translation="MRIMAQVGMVMNLDKCIGCHTCSVTCKQAWTNRAGTEYVWFNNV
                     ETRPGLGYPRRYEDQDRWQGGWVRTKSGGLKLRSGGRFQKLLSIFASPVQPELDDYYE
                     PWTYDYANLIQAPLGDDFPVARPKSLITGEDTKITWSANWDDSLGGVAETGQSDPVIE
                     KLRDEANEQIRFEYEKSFMFFLPRICEHCLNPSCMASCPSGAIYKRAEDGIVLVDQDR
                     CRGWRQCITGCPYKKMYFNHRSGKAEKCTFCYPRIEVGLPTVCSETCVGRLRYLGIVF
                     YDADKVLEAASVPDDQLYQSQLDLLLDPRDPAVVAEARAAGMPDDWIAAAQRSPIYAL
                     IKHFKVALPLHPEYRTMPMVWYIPPLSPVTDLLKAQGHDAEAPGNLFGAIDALRIPVS
                     YLAELFTAGDTTIVTDVLQKLAAMRSYMRGVTLGTGRDAELAAAVGMTPEAMEQMYRL
                     LAIAKYDERYVIPKAHREEAHNLEELACSLDFEGGPGMYESGPFGEASGRPVPVAIET
                     YSALKARQQAEAAASSEQLHHREGLLNWDANEDAKP"
     gene            complement(6664..>8681)
                     /locus_tag="PLF56_03055"
     CDS             complement(6664..>8681)
                     /locus_tag="PLF56_03055"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_002549317.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_function: GO:0016491 - oxidoreductase activity
                     [Evidence IEA]"
                     /codon_start=3
                     /transl_table=11
                     /product="molybdopterin-dependent oxidoreductase"
                     /protein_id="HOQ52573.1"
                     /translation="IGRNGGGWAHYVGQEKCRPMTGWFNLANALDWSRPPRTMIGTGY
                     WYMHTDQWRTDGYSADRVKSPLSTGKLDGLHTADAMALSHRLGWMPFYPQFDRNPLDL
                     ADEAKAAVEAGEAENVGAWVAGKLKDGTLTTSLEDIDAPENWPRTMVVWRSNLFGSSA
                     KGNEYFLKHLLGTQSNLMPNEHAAEAKPTVVKFRDEAPEGKLDLLISADFRMTSTTLL
                     SDIVFPAATWYEKYDLSSTDMHPFVHAFTPAIDPPWEAKSDFEFFQLLAQAISTLAKG
                     RLDTRSDLVAVPMQHDTPGQISQPGGHVPDWRGTDLPAVPGRNLPALMVVERDYTAIA
                     DKLLSVGPLADRLGFTVKNITYDVKHSVELLGRLHGVMPSGTAAGRPAIDTDARLAEA
                     ILAFSGTTNGELATQGFHDLEAKTGRPLVDLSEGQEERRITFKQTQEAPQPVITSPEW
                     SGSETGGRRYAAFTINTERLKPWHTLSGRMHFFLDHDWIIDAGEQLPTFRPPLDMSRA
                     FGEPELGPTGEKAITLRYLTVHNKWSIHSEYQDNLFMLSLGRGGPQAWLSVADAQSIG
                     VADNDWIELTNANGVFVARAVVTHKLPDGICYVQHAQERTIDVPKSEATGKRGGIHNS
                     VTRILVKPTHLIGAYAHLSYAFNYLGPTGVQRDIVSTVRRRSQEVTY"
BASE COUNT         1450 a         2834 c         3024 g         1373 t
ORIGIN      
        1 gtctcggggt cggtgaccac atcgagcacc tcggcatgac cggccaccac ccagcgaccg
       61 gcggcgtccc tgctcagcgg gcacccgtgc agggcgtcgt aggcaatgcg cggatcagac
      121 atgaccggct ccgtccagtt gttcgaggat gtacccgatc aggtcgccga ccacgccgag
      181 cgtgtcacga atgccgcccc gcgaaccggg ggccgccagc agcagtaccg aaggagcctc
      241 gtcgcgcagc accacgccgg ccacctcgcg tgacaccagg gccagccgag tgtgggcgct
      301 gccgcggcgc cggatctctt cgccgatgcc gggaatctcg acatcgatca gggcccggac
      361 gacgtcgctg gtccagtccc gcggcccgat gccggtgccc ccgcaggcca gcaccacccg
      421 cgcgcccgaa tcgatcgcgg caccgatcgc ggcgcgcagg cgtccaggat catccggcac
      481 catacaccga tccgccggca ccccgtgcgc ggccagcagg tccaccgcca ccgggccgcc
      541 gcggtcggtg tcggcacccg aggcgacctc gtccgacacc acgatcacgg tggcccgcag
      601 ccccgcggcg ctgcgcaggg cggcttcggc agcggccgcg acgtcggtgg tccttgttct
      661 ctcggccatg gctcaggact tcttcggtgc cccgggacgg gcgtagcgcc accaggtgat
      721 gccgacgcac atgatcgccc acgccacgcc gatccagtag aaggtggccg gcgccatgat
      781 ggtcagcccg acgccgaaca ggaacgggcc gaaggcggcg atggcagccg tccaaccgat
      841 cacaccaccg gcctggcggc gctcgaagat catcggcatc tgcttgaacg tggacgcgtt
      901 gccgatgccc gagaacagga agatcagcag catgccccac agaaagtggt tgaagccggc
      961 ctgcagggtg gcggcgctgg tcaggtcggg ggtgagcgag ggcaccgtcc aggcgatcga
     1021 agcggccagg ccgatgcctg agatgagcgt ccagatggcc ccgcccatgc ggtcggtgag
     1081 cggcgagaac agcacccgcg cgccggcgcc gaccagcgga ccgaggaaga cgtaggtggc
     1141 cgggtcgggc acctggtagc cggcgaccag aatctgtgcg gtcgagcccg cgccctgcac
     1201 gatgccggcg ttgccggagc cgtacaggtt gatcatcagc agcccgaact gggccgacag
     1261 gcccgagaag gtgccgaagg tcatgatgta gaggatcgtc atccaccagg tgtcctggtt
     1321 gctgaagatg tcgaactgct gcttgatgtt ggccttgatc ggcactgatt tcaggtactg
     1381 ccaggcgatc caggtgccga tcaggatgaa cggcagccag acgaacgacg ctgcctggta
     1441 ccagacctca cggtcgggct tgcctgcggt cttgagcatc tggctctgac ccatccacca
     1501 caacatgccg gtgccgatca gccagggggt gagcagctgc accagcgaga cgccgaagtt
     1561 gccgatgccg gcctgcaggc ccagtgcggt gccctgcttg ctgcgcggaa agaagtacga
     1621 cgtggacggc atgaagcccg agaaggcgcc gccgccgatg ccggccagga aggccagcac
     1681 gagcagctcc cagtaggggg cggtcggcga catcgagcgc acgccccagc cgagcaccgg
     1741 aaagaacagc agcagggtgg tggccgccac catcttgcgg gtgcccatga tcggcggcag
     1801 caccatccac agcagccgca tcaggccgcc ggacaggccg ggaacggctg ccagccagta
     1861 cagctgctcc ttggtgaagc tgtagccgat ggcgttcagc ttcggaacga tcgccgaggg
     1921 taggaaccag gtgacgaagc acatggtcag gctgaaggtg gtgatccaca gggtctgcca
     1981 ggccagtttg ctgttccagg tctcgtcgtt ctcgggatcc caggcccgca gccactcggg
     2041 cccaccctgc agtttctcca tcatggtgct catgctccga cctcctggcg ggaacgtgtg
     2101 tcgatgtgtt cggtgtcgaa gtgatcggcc aactcgggcg actgctcgtg cagcatgccg
     2161 acgatggtcc agtgcatcca gaccgcgcag atcgcggtga ggacgaacag cacgaagaag
     2221 gtactggtcg ggaaccccgt ccaggccttg gtgtaggcga acagcggggg caggatgaag
     2281 ccgcccaggc cgcccagcat gccgaccagg ccgccgaccg agccgacgtt gtcgggaaag
     2341 tactcgggaa tgtgcttgta gaccgcggcc ttgccgatgc ccatcgagca gcccagcagg
     2401 aacaccagca cggtgaacca gaccacgttg atgccgtagc ccaggtgctc ggtctgggtg
     2461 ccgtcggcgt gggcgatgac gatgtgtccg ttgggcatca tcagaatgcc ggtggtgacc
     2521 agcatcaccg cgaacgtgac gtacatcacc ttgcgggcgc ccagtttgtc gctgacccag
     2581 ccaccgaccg gacgcagcag cgaggcggga aagatgaaca gggcggtcag cagggcggcg
     2641 gtgtagaggt cgaccttgaa gttgtcgacg tagtacttgg gcagccaggc cgacagggcg
     2701 acgtacgcgc cgaacacggc cacgtagtag aggctgaacc gccacacccg caggtgcttc
     2761 aggggcacca gcatctcgcg gatcggcttg cccggcccgg ccatgcggtc atgccggggc
     2821 accaccagaa aggtgatcac ggcgagtgcc agcagcagca ccgagtagat caccggcacc
     2881 agtcgccagc cgccctgcac gccgaacgcg aacgtcatac ccgcggtgcc ggcgatcagc
     2941 gggggaccga tgaacttggt gatggacgcc cccacgttgc ccgcgccgaa caggcccagg
     3001 gcgaaaccct gccgttcgcg gccgaaccag gcggcgttcc aggccacgcc gacgctgaac
     3061 aggttgccgg cgaagccgac cagaaaggcc agcagcagca gcattccgta gctgtgcgcg
     3121 gtgctgacca ggtaggcggg taccgcggtg gcggccagca tgaagatggt caccttgcgt
     3181 ccgccgatgc ggtcggcgat gatgccggcc ggcagccgcc acatcgagcc gttcagcacg
     3241 gccagtgcgg tgatccacga cagctgcacg tcgtcgagca gcagttcctt ctgaatctcg
     3301 atgcccagga tgccgaacat cagccacacg gcgaacatca cggtgaagtc gatcgtcgac
     3361 atcgccagga cgcggttggc gccgcgccca gtgtcgtgca ccggcggaac cacgtcctcc
     3421 gccggcgggg tgggggtgct catggtttgg tgcgctcgcg atccttggtg ccgatgggat
     3481 cccagccgcg gacgcgtccg tgcgagggag cggcgggggc gtcatcgcga ctgcggtaga
     3541 cgacgtaggg gcggaacagg tagtgcagcg gcacggtgaa ggcgtgcacc aggcgactga
     3601 acggaatcag ggcgatcagg gcgaagccca tcagaatgtg caccttgaac tgccaggtgc
     3661 tctcggccat cgcctcgacg ttgggctgaa agaccagcag tgagcggaac cacaccgaca
     3721 ccgtctcgcg gtagttgtgg tgctcgccgg tgggggtgtg gtcaccggtc agggtggcga
     3781 gcatgccgag cacgatcgcc gcgaccagaa tcacgtacat cagcttgtcg ttgcgggtgg
     3841 tggccaggaa caccgggccg gtggtgcggc gacggtagat cagcagtgcg ataccggcca
     3901 gggtggccac gccggcgacg gtgccggcgc ccagtgcgac cgcgtggtag gcctcctggc
     3961 tgaccaccag gtcggtccag gccttcggga tcagcaggcc catcaggtga cccatcagca
     4021 ccacgaacag gccgtagtga aacatcgggg acgcgatgcg cagcagccgt gattcgtaca
     4081 gctgcgagct gcgggtggtc cagccgaact ggtcgtactt gtaacgccag atcgtgccgg
     4141 cgatcagcac cagcgccacc aggtagggca gcacgcccca cagcatgatg tcgagcgcat
     4201 tcatgtcatg accttccctt gggcgaagat cccgggtcgc ggcccgggat gacggcatcg
     4261 gcggtgccgg tctgggtcgg actcgtcatc ccggcgaaag ccgggacccc gttcaccgcg
     4321 acgagatcca ggccgaccag ctcgaccggt tgcgtctgcc cgaagcgggc ctgaacctcg
     4381 gcacggttgc gcggcgactc gcccggcaac cgggtgcaga tcgcagtcag cactccggcg
     4441 tgcggcaggt ggtcggccgc cagttcgagg cggatcaact ccaggctggc ccggaagcgt
     4501 tcgagcagcg cgaacccgcg ctcggggtcg tccaccgcga actcgagcac catcggcagg
     4561 tagtcgggca gttcgccgcc ggtgtcgacc accagccccg agtcgcggta gacctgcttg
     4621 atctcggcca gcacgttgcc ccggcgccgg gtgtcaccgt cggtccagta gctcaggtgc
     4681 agcgcgtgcc ggcgggacag gtcgaactcc tgcacatgga acgactgcag ttcgcgcagg
     4741 gagtcgcctc gcaggtgggc cagcacgggg gcgaagagcg gctcgtccgt tgtgccggaa
     4801 agtgccgact cgatgacgtc caggcggtcg agcagtgcgg cctcggggta ggccaggacg
     4861 agtgccgcgg cctggtagac gagggcgtcg ccccgggttc gacccgagat cccggcgttc
     4921 gccgggatgt caggccggga cggagtgtcg ggccgggtcg ggtcgtcgtc gccccttccg
     4981 tcatcccggt gaacgccggg atctcgccgc agccactcgg tcagtcgcct catggcttgg
     5041 cgtcctcgtt ggcgtcccag ttcagcaggc cctcccggtg gtgcagttgc tcggaggacg
     5101 cggccgcctc ggcctgctgg cgcgccttca gcgccgagta ggtctcgatc gcgaccggca
     5161 ccggacgtcc ggacgcctca ccgaacggac ccgactcgta catgcccgga cccccctcga
     5221 agtcgagcga acaggccagc tcctcgaggt tgtgcgcctc ttcacggtgc gccttgggaa
     5281 tcacgtaacg ctcgtcgtac ttggcgatcg ccagcagccg gtacatctgc tccatggcct
     5341 cgggggtcat gccgacggcc gcggccagtt cggcgtcgcg tccggtgccc agggtgaccc
     5401 cgcgcatgta gctgcgcatc gcggccagct tctgcagcac atcggtgacg atcgtagtgt
     5461 cgccggcggt gaacagctcg gccaggtagc tgaccggaat gcgcagcgcg tcgatggccc
     5521 cgaacaggtt gccgggcgcc tcggcgtcat gaccctgcgc cttcagcagg tcggtgaccg
     5581 gtgacagcgg cgggatgtac cagaccatcg gcatggtgcg atattccgga tgcagcggca
     5641 gcgccacctt gaagtgcttg atcagggcgt agatcggcga gcgctgggca gcggcgatcc
     5701 agtcgtccgg catgccggcg gcgcgggctt cggcgaccac tgcggggtcg cgcgggtcga
     5761 gcagcagatc cagttgtgac tggtacagct ggtcatcggg aacggacgct gcctcgagca
     5821 ccttgtcggc gtcatagaac acgatgccca ggtaccgcag ccggccgaca caggtctcgg
     5881 agcacaccgt cggcagcccc acctcgatgc gcgggtagca gaacgtgcac ttctcggcct
     5941 tgccggagcg gtggttgaag tacatcttct tgtacgggca gccggtgatg cactggcgcc
     6001 agccgcggca gcggtcctgg tcgaccagca cgatgccgtc ctcggcccgc ttgtagatgg
     6061 cgcccgaggg gcaggacgcc atgcacgacg ggttcaggca gtgctcgcag atgcgcggca
     6121 ggaagaacat gaagctcttc tcgtactcga agcgaatctg ctcgttggcc tcgtcacgca
     6181 gcttctcgat caccgggtcg gactggccgg tctcggcgac gccgcccagg gagtcgtccc
     6241 agttggccga ccaggtgatc ttggtgtcct cgccggtgat cagcgacttg ggccgggcca
     6301 ccggaaagtc gtcgcccagc ggcgcctgga tcaggttcgc gtagtcatag gtccagggct
     6361 cgtagtagtc gtccagctcg ggctgcaccg gcgaggcgaa gatgctgagc agcttctgaa
     6421 accgcccgcc gctacgcagt ttgaggccgc cactcttggt gcgcacccag ccgccctgcc
     6481 acctgtcctg atcttcgtag cgtcgtgggt agcccagccc gggccgcgtc tcgacgttgt
     6541 tgaaccagac gtattcggtg ccggcccggt tcgtccaggc ctgcttgcag gtgactgaac
     6601 aggtgtggca accgatgcac ttgtcgaggt tcatcaccat gccgacctgc gccatgattc
     6661 tcatcagtac gtgacctcct gggaacggcg gcgaacggtg gagacgatgt cgcgttgcac
     6721 cccggtgggg ccgaggtagt tgaacgcgta actcagatgg gcgtaggcgc cgatcagatg
     6781 ggtcggcttg accagaatgc gggtgaccga gttgtggatg ccgccgcgct tgccggtggc
     6841 ctccgacttg ggcacgtcga tggtgcgttc ctgcgcatgc tgcacgtagc agatgccgtc
     6901 cggcagcttg tgggtgacca ccgcccgcgc cacgaacacc ccgttggcgt tggtcagttc
     6961 gatccagtcg ttgtcggcca ccccgatcga ctgggcgtcg gcgacgctga gccaggcctg
     7021 gggcccaccc cgtccaagcg acagcatgaa caggttgtcc tggtactcgg aatggatcga
     7081 ccacttgttg tgcaccgtca ggtagcgcag ggtgatcgcc ttctcgccgg tcgggcccag
     7141 ttcgggttcg ccgaaggcgc ggctcatgtc cagcggcgga cggaacgtgg gcagctgctc
     7201 gcccgcgtcg atgatccagt cgtggtcgag gaagaagtgc atccggcccg agagggtgtg
     7261 ccagggcttg agccgctcgg tgttgatggt gaaggcggcg tagcgccgtc caccggtctc
     7321 ggagcccgac cactccggtg aggtgatcac cggctgcgga gcctcctggg tctgtttgaa
     7381 cgtgatccgg cgttcctcct ggccctcgga caggtcgacc agcggccgtc cggtcttggc
     7441 ctccagatcg tggaagccct gcgtggccag ttcgccgttg gtggtgcccg agaacgccag
     7501 gatcgcctcg gccagccggg cgtcggtgtc gatcgccgga cgtccggcgg cggtgcccga
     7561 gggcatcacg ccgtgcagcc ggccgagcag ctcgaccgag tgcttcacgt cgtaggtgat
     7621 gttcttcacg gtgaatccga gccggtcggc cagcggaccg accgacaaca gcttgtcggc
     7681 gatcgcggtg tagtcgcgct cgaccaccat cagcgccggc aggttacgac cgggcacggc
     7741 gggcaggtcg gtgccgcgcc agtcgggcac gtgcccgccg ggctggctga tctgcccggg
     7801 ggtgtcgtgc tgcatcggca ccgcgaccag gtcggatcgg gtatcgagcc gacccttggc
     7861 cagcgtcgag atcgcctgcg ccaacagctg gaagaactcg aaatcgctct tggcctccca
     7921 cggcgggtcg atcgccgggg tgaacgcgtg cacgaacggg tgcatgtcgg tgctgctcag
     7981 gtcgtacttc tcgtaccagg tggcggccgg gaagacgatg tcggacagca gcgtggtcga
     8041 ggtcatccga aagtcggcgc tgatcagcag gtcgagcttg ccctcgggcg cctcgtcccg
     8101 gaacttcacc accgtcggct tcgcctcggc cgcgtgctcg ttgggcatca ggttggactg
     8161 cgtgccgagc aggtgcttca gaaagtactc gttgcccttg gcggacgacc cgaacaggtt
     8221 cgagcgccag accaccatgg tgcgtggcca gttctcgggg gcgtcgatgt cttcgagcga
     8281 ggtggtcagg gtgccgtcct tgagcttgcc cgccacccag gcgccgacgt tctccgcctc
     8341 gcccgcctca accgccgcct tcgcctcgtc ggccaggtcg agcggattgc ggtcgaactg
     8401 cggatagaac ggcatccagc cgagccggtg gctgagcgcc atcgcgtcgg cggtgtgcag
     8461 cccgtccagc ttgccggtgg acagcggcga cttgacccgg tcggccgagt agccgtcggt
     8521 gcgccactgg tcggtgtgca tgtaccagta gccggtgccg atcatggtgc gcggcggacg
     8581 ggaccagtcc agcgcgttgg ccaggttgaa ccagccggtc atcggacggc acttctcctg
     8641 gccgacgtag tgcgcccagc cgccgccgtt gcgtccgatg c
//
LOCUS       DAOSUG010000056         8485 bp    DNA     linear   ENV 10-JUN-2024
DEFINITION  MAG TPA_asm: Micropruina sp. isolate AMR_MDS_2195
            NODE_4357_length_8485_cov_4.209015, whole genome shotgun sequence.
ACCESSION   DAOSUG010000056 DAOSUG010000000
VERSION     DAOSUG010000056.1
DBLINK      BioProject: PRJNA925477
            BioSample: SAMN32864262
            Sequence Read Archive: SRR4236673
KEYWORDS    WGS; ENV; Metagenome Assembled Genome; MAG; Third Party Data; TPA;
            TPA:assembly.
SOURCE      Micropruina sp. (wastewater metagenome)
  ORGANISM  Micropruina sp.
            Bacteria; Bacillati; Actinomycetota; Actinomycetes;
            Propionibacteriales; Nocardioidaceae; Micropruina.
REFERENCE   1  (bases 1 to 8485)
  AUTHORS   Abdulkadir,N., Saraiva,J.P., Zhang,J., Stolte,S., Gillor,O.,
            Harms,H. and Rocha,U.
  TITLE     Genome-centric analyses of 165 metagenomes show that mobile genetic
            elements are crucial for the transmission of antimicrobial
            resistance genes to pathogens in activated sludge and wastewater
  JOURNAL   Microbiol Spectr 12 (3), e0291823 (2024)
   PUBMED   38289113
REFERENCE   2  (bases 1 to 8485)
  AUTHORS   Abdulkadir,N.
  TITLE     Direct Submission
  JOURNAL   Submitted (24-MAR-2023) Environmental Microbiology, Helmholtz
            Center for Environmental Research -UFZ, Permoserstr 15, Leipzig
            04318, Germany
COMMENT     The annotation was added by the NCBI Prokaryotic Genome Annotation
            Pipeline (PGAP). Information about PGAP can be found here:
            https://www.ncbi.nlm.nih.gov/genome/annotation_prok/
            
            ##Genome-Assembly-Data-START##
            Assembly Method        :: SPAdes v. 3.11.1
            Genome Representation  :: Full
            Expected Final Version :: Yes
            Genome Coverage        :: Not Applicable
            Sequencing Technology  :: Illumina MiSeq
            ##Genome-Assembly-Data-END##
            
            ##Genome-Annotation-Data-START##
            Annotation Provider               :: NCBI
            Annotation Date                   :: 05/04/2023 04:57:10
            Annotation Pipeline               :: NCBI Prokaryotic Genome
                                                 Annotation Pipeline (PGAP)
            Annotation Method                 :: Best-placed reference protein
                                                 set; GeneMarkS-2+
            Annotation Software revision      :: 6.5
            Features Annotated                :: Gene; CDS; rRNA; tRNA; ncRNA
            Genes (total)                     :: 2,776
            CDSs (total)                      :: 2,750
            Genes (coding)                    :: 2,714
            CDSs (with protein)               :: 2,714
            Genes (RNA)                       :: 26
            tRNAs                             :: 24
            ncRNAs                            :: 2
            Pseudo Genes (total)              :: 36
            CDSs (without protein)            :: 36
            Pseudo Genes (ambiguous residues) :: 0 of 36
            Pseudo Genes (frameshifted)       :: 2 of 36
            Pseudo Genes (incomplete)         :: 32 of 36
            Pseudo Genes (internal stop)      :: 2 of 36
            ##Genome-Annotation-Data-END##
FEATURES             Location/Qualifiers
     source          1..8485
                     /organism="Micropruina sp."
                     /mol_type="genomic DNA"
                     /submitter_seqid="NODE_4357_length_8485_cov_4.209015"
                     /isolate="AMR_MDS_2195"
                     /isolation_source="Activated sludge of municipal
                     wastewater treatment plants"
                     /db_xref="taxon:2737536"
                     /environmental_sample
                     /geo_loc_name="Canada"
                     /lat_lon="49.25 N 123.1 W"
                     /collection_date="2015-06-16"
                     /metagenome_source="wastewater metagenome"
                     /note="metagenomic"
     gene            <1..249
                     /locus_tag="PLF56_03060"
     CDS             <1..249
                     /locus_tag="PLF56_03060"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_013862672.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_function: GO:0016491 - oxidoreductase activity
                     [Evidence IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="FAD-dependent oxidoreductase"
                     /protein_id="HOQ52574.1"
                     /translation="PTDIVTEQLVTPNDWLSQGMAAGTPFALAHTFAQTGPFRPRNVE
                     KRVPGLVFAGSSTTPGVGIPMVLISGKLAAQRVTEVLS"
     gene            246..1157
                     /locus_tag="PLF56_03065"
     CDS             246..1157
                     /locus_tag="PLF56_03065"
                     /EC_number="2.5.1.-"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_013862673.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_function: GO:0004659 - prenyltransferase activity
                     [Evidence IEA];
                     GO_function: GO:0046872 - metal ion binding [Evidence
                     IEA];
                     GO_process: GO:0008299 - isoprenoid biosynthetic process
                     [Evidence IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="phytoene/squalene synthase family protein"
                     /protein_id="HOQ52575.1"
                     /translation="MTRLDAGYARCAELTRKHGTTYYWGTLLLPRAQRREVFAVYALC
                     RLADDIVDEPDTVQPEDTAVPRDGTPAERLDAFRRAFFAALEAGDAEQPVLAAIVDSI
                     RRTGTDPECFERFFDAMALDLTRTTWASWPELRDGYMEGSAAVIGEMMVPVLEPLSQG
                     AFEPARSLGLAFQLTNFIRDVGEDLDRGRVYLPADELAAHGADPWLRRVTPQWREFLA
                     AQIARNRELYEAARPGIGLLPPGSARCVGAAHALYSQILDRIEAADYDVFSERRRVPR
                     VTKAATVARLMLTRPDANAATPALSAR"
     gene            1167..1742
                     /gene="dcd"
                     /locus_tag="PLF56_03070"
     CDS             1167..1742
                     /gene="dcd"
                     /locus_tag="PLF56_03070"
                     /EC_number="3.5.4.13"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_011757838.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_function: GO:0008829 - dCTP deaminase activity
                     [Evidence IEA];
                     GO_process: GO:0009394 - 2'-deoxyribonucleotide metabolic
                     process [Evidence IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="dCTP deaminase"
                     /protein_id="HOQ52576.1"
                     /translation="MLLSDRDIRAQIDAGRVQFEPWDPAMIQPSSVDVRLDKFFRLFD
                     NHKYPVIDPAADQPELTRLVEVVPSEGFVLHPGEFVLGSTYETITLPDDLAARVEGKS
                     SLGRLGLLTHATAGFVDPGFSGHVTLELSNVATLPIMLWPGMKIGQLAFFQLSSPSEN
                     PYGSAVYGSHYQGQRGPTASRSFQNFSRTDV"
     gene            complement(2342..2725)
                     /locus_tag="PLF56_03075"
     CDS             complement(2342..2725)
                     /locus_tag="PLF56_03075"
                     /inference="COORDINATES: protein motif:HMM:NF013739.2"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_function: GO:0016491 - oxidoreductase activity
                     [Evidence IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="FAD-dependent oxidoreductase"
                     /protein_id="HOQ52577.1"
                     /translation="MLFVDADGHGPLVNTAVVSNVAPGYAPSGQHLVQCSAVLEGAAP
                     SEPEVRAHASAIYDGPTDDWRLLAVHEVQHSLPAITPGTQRPPCDLGGGVFVAGDFVE
                     GASIQGALLSGRRTAEAVLQSLATG"
     gene            complement(2762..3550)
                     /locus_tag="PLF56_03080"
     CDS             complement(2762..3550)
                     /locus_tag="PLF56_03080"
                     /inference="COORDINATES: protein motif:HMM:NF013739.2"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_function: GO:0016491 - oxidoreductase activity
                     [Evidence IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="FAD-dependent oxidoreductase"
                     /protein_id="HOQ52578.1"
                     /translation="MDADVIVIGAGLAGLRAARVLAEAGRSVLVLEAADRAGGRVATD
                     EVDGFLLDRGFQLINPAYAELRDAVQVADLDLRQFQRGVAVRDVDGLTVLADPVRHPV
                     LAVRAAGSRYLRPAQVGGALAWLRGSDTRADDRTLAASLDRAGFSGPLREAVEQFLVG
                     VLADRTGSTSAAFVRSLIGWFLRGTPGLPSDGMRALPRLLAEGLSVRLEASVDQVRRT
                     PDGVRVAIADGSELAARAVVVAVDPVAVGELTSLPPVPMRGLVT"
     gene            complement(3553..5076)
                     /locus_tag="PLF56_03085"
     CDS             complement(3553..5076)
                     /locus_tag="PLF56_03085"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_013862677.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_function: GO:0016491 - oxidoreductase activity
                     [Evidence IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="FAD-dependent oxidoreductase"
                     /protein_id="HOQ52579.1"
                     /translation="MIGRPPRRLPGRDRHAVRHPAQGGVARVREPRHVIVVGGGIAGL
                     AAATVLAERGVRVTVLEAQQQLGGRVRAWPLPDGRTMSRGFHAFFRQYYNLRALLRRT
                     DPSLDRLVPIEDYPLTRPDGLTDSFTSLPRTPPWSILGFVLASPTFRLRDLTRVDVSS
                     ALELLQVRFPGSYARYDGESAAAFLDRLRFPAAARDLALEVFARSFFADPREFSAGEL
                     VAMFHTYFLGSAEGLLFDVPDDDYDTALWAPLGGYLAGLGVEVRTGARVESLDPSAGR
                     VIVDGAELAADGLVLALDPRSARTLVGELDTATDTPTATWTQRVAATHNAPPFAVLRL
                     WLDRPVAAERPAFVGTSGFGPLDNISVLDRFEAGAARWAAASGGSVVELHGYALYDAN
                     NIEQRLLDELHRAWPETASAGIVAREMLVEDDCGLIGLSPWAERPGVHTPWPGLVLAG
                     DWVRCDLPVALMERAATTGTLAANALLAQWGVAGEEVWSVPMAGLLGAYAPTTPTKE"
     gene            complement(5073..5945)
                     /locus_tag="PLF56_03090"
     CDS             complement(5073..5945)
                     /locus_tag="PLF56_03090"
                     /EC_number="2.1.1.-"
                     /inference="COORDINATES: protein motif:HMM:NF013382.2"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_function: GO:0008168 - methyltransferase activity
                     [Evidence IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="class I SAM-dependent methyltransferase"
                     /protein_id="HOQ52580.1"
                     /translation="MKIEAASTNGGTTAQAPVRSAVRGDTPKAPAALTHASDDVTREF
                     DAGAPRYDLLVGLNPGYHRHLRRAADALADLVPAASRLVDLGCGSGASTRALVSTFGE
                     GVEIQGVDASAGMLAQARGKTWPRGVSFTQGRAGELTPLLDAPADGILAAYLFRNVPE
                     ADRDAALADAWAALRPGGALVVLEYSVAGDARAAQVWRAVCEAVILPLATLTGGNHGL
                     YRYLERSVLEFDTVPRFVARLHRVGFVDVESCTVPGWQRGILHVIRARKPLVEAASTI
                     DHGDREPRGERGPS"
     gene            complement(5954..6253)
                     /locus_tag="PLF56_03095"
     CDS             complement(5954..6253)
                     /locus_tag="PLF56_03095"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_013862679.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_function: GO:0016872 - intramolecular lyase activity
                     [Evidence IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="lycopene cyclase domain-containing protein"
                     /protein_id="HOQ52581.1"
                     /translation="MGEYTLFTLIAVLGVLALEFFWLRTGLLRTAQYWLAMALVFFFQ
                     IWVDGWLTKLSDPIVIYYPDQYSGIRFPFDIPIEDFGFGFAMVTLTLLVWHRLTH"
     gene            complement(6246..6614)
                     /locus_tag="PLF56_03100"
     CDS             complement(6246..6614)
                     /locus_tag="PLF56_03100"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_013862680.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="lycopene cyclase domain-containing protein"
                     /protein_id="HOQ52582.1"
                     /translation="MDHYQYLLLMAACLLITLPLEIVLGARVYARWRLMLLALLPTVL
                     IFSIWDVVGIIRLHWTYNPAYISGIHLGVMPLEELVFFIVIPICGLLTYEAVGTVLRR
                     LRQRGDGVRSNDGVRSSDDG"
     gene            complement(6952..8070)
                     /gene="ald"
                     /locus_tag="PLF56_03105"
     CDS             complement(6952..8070)
                     /gene="ald"
                     /locus_tag="PLF56_03105"
                     /EC_number="1.4.1.1"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_016669452.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_component: GO:0005737 - cytoplasm [Evidence IEA];
                     GO_function: GO:0000286 - alanine dehydrogenase activity
                     [Evidence IEA];
                     GO_process: GO:0006522 - alanine metabolic process
                     [Evidence IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="alanine dehydrogenase"
                     /protein_id="HOQ52583.1"
                     /translation="MRIAVPSEVKNREYRVAITPVGVNELVHRGHQVFVQAGAGLGSS
                     ITDEEYADQGATILPDADATWAEGEMVMKVKEPIESEYGYLRDDLLLFTYLHLAADRP
                     LTEALLTTSTTALAYETVQLPNGMLPLLYPMSEVAGCLAPQVGAHSMMKAQGGRGVLM
                     GAVGGVPNAKVVVLGGGVAGQNAANIALGMGANVTILDTDLEKLRMTFWRYDNRIQGI
                     VSTALSVREQVLAADLVIGTVLIPGAKAPKLVTNDMVAAMKPGSVLVDVAIDQGGCFE
                     DSHPTTHDDPTFTVHESVFYCVANMPGAVPNTSAWGLTNATLPYAVQLADKGWRRACD
                     ESQALALGLNTVGGAVTCQGVSNAFGDLPRTSVRNVLD"
     gene            8265..>8485
                     /locus_tag="PLF56_03110"
     CDS             8265..>8485
                     /locus_tag="PLF56_03110"
                     /inference="COORDINATES: protein motif:HMM:NF017984.2"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_function: GO:0019104 - DNA N-glycosylase activity
                     [Evidence IEA];
                     GO_process: GO:0036297 - interstrand cross-link repair
                     [Evidence IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="crosslink repair DNA glycosylase YcaQ family
                     protein"
                     /protein_id="HOQ52584.1"
                     /translation="MLSWSDLAARTLARQFPVEVEPGDVQGLLARVGPIQSQTARSVF
                     LGVAARLPGVTREQLTAAYESSQIVRGSTL"
BASE COUNT         1325 a         3074 c         2911 g         1175 t
ORIGIN      
        1 ccgacggaca tcgtcaccga acagctggtg acgcccaacg actggctgag ccaggggatg
       61 gccgcgggca ccccgtttgc gctcgcgcac acgttcgccc agaccgggcc gttccgtccg
      121 cgcaacgtcg agaagcgcgt cccgggactc gtctttgcag gatcctcgac cacgccgggc
      181 gttggcattc ccatggtgct gatctcgggg aagctggccg cgcagcgggt cactgaggtg
      241 ctgtcgtgac gcgcctggac gccggctacg cccgctgcgc cgagctgacc cgcaagcacg
      301 ggacgaccta ctactggggc acgctgctgc tgcctcgcgc gcagcggcgc gaggtgttcg
      361 cggtgtacgc gctgtgccgc ctagccgacg acatcgtcga cgagcccgac accgtgcaac
      421 ccgaggacac cgcggtgccg cgggatggca cgccggctga gcggctggac gccttccgcc
      481 gggccttctt cgcggccttg gaagcagggg acgccgagca gccggtgctg gccgccatcg
      541 tcgacagcat tcggcgcacc ggcaccgacc ccgagtgctt cgagcgcttc ttcgacgcga
      601 tggcgctcga cctcacgcgc acgacgtggg cgtcctggcc cgaactgcgc gacggctaca
      661 tggagggctc ggcggccgtc atcggcgaga tgatggtgcc ggtgctcgag ccgctgtcgc
      721 agggcgcctt cgagccggcc cgctcactgg gcctggcctt ccagctgacc aacttcatca
      781 gggacgtcgg tgaagacctc gaccgtggcc gggtgtacct gccggccgac gaactggcgg
      841 cgcacggcgc cgatccgtgg ctgcggcggg tgaccccgca gtggcgggag tttctggcgg
      901 cgcagatcgc ccgcaaccgc gagttgtacg aggccgcccg gccgggcatc gggctgctgc
      961 cgcccgggtc ggcccggtgc gtgggggcgg cgcacgcgct gtactcccag atcctcgacc
     1021 ggatcgaggc ggccgactac gacgtgttca gcgaacgccg ccgggtgccg cgggtcacca
     1081 aggcggcgac ggtcgcccgg ctgatgctga cccgtccgga cgcgaacgcc gcgacgccgg
     1141 cgctgtctgc gcgatagcct cgcggggtgc tgctttccga ccgcgacatc cgcgcccaga
     1201 tcgacgccgg gcgcgtgcag ttcgagccgt gggatccggc gatgatccaa cccagcagtg
     1261 tcgatgtgcg gctggacaag ttcttccggc tgttcgacaa ccacaagtac ccggtgatcg
     1321 acccggccgc cgaccaaccg gaactgaccc ggctggtcga ggtggtgccg tccgagggtt
     1381 tcgtgctgca cccgggtgag ttcgtgctgg gcagcaccta cgaaaccatc acgctgccgg
     1441 acgacctggc cgcccgggtc gaaggcaagt ccagcctcgg acgcctcggg ctgctcaccc
     1501 acgccaccgc cggcttcgtc gaccccggtt tcagcggcca cgtgacgctg gaactgtcga
     1561 acgtggccac cctgccgatc atgctgtggc cgggcatgaa gatcggccag ttggcgttct
     1621 ttcagctcag cagcccgtcc gagaacccgt acggatcggc ggtgtacggc tcgcactatc
     1681 aggggcaacg cgggccgacc gccagccgca gctttcagaa cttctcccgg acggacgtgt
     1741 agtcaccggc gccccggccc ctgtcatccc gaccccctgt catcccgacc ccccgtcatc
     1801 ccgacccccg tcatcccggc gaatgccggg atctcgtccg gtgccccgat gtcaccggct
     1861 tcccggcgaa cggcgcatcc ggcgccgatg ctgaccctcc ggccccgttc ctcccggacc
     1921 ccccccgtca tcccggccct cgtcatcccg gcgaatgccg cgatctcgtg cggagccacg
     1981 atgtcgcgcc gaggaaccgc tgggttgaga ccatcgtgtc gtgggcggtt gggtcggtcg
     2041 ccgcgcggct ggcgtgatcg ttcgcgacgg tccgtggttg cagtgcctgc ccgcaatccc
     2101 tgaaatccct gcaccagtaa cagacccagg aggcgtccac gccgggagag cccgcactgg
     2161 tgcaggcttt tcaggcggtg gcccaccagc gtcggttctg ctgcaaggcc cccgtccgcc
     2221 ggtctgccgc tcaccgagta atcgacgttg cgcggtcaag ggctggttgc gcgggttcgt
     2281 ccgcgcgcga tgagcgctcc gccgcgcaac gtcacccacc gtgacctcga acgacactgc
     2341 ctcagccggt cgcgagactc tgcagcacag cctcggccgt gcggcgtccg gagagcaggg
     2401 cgccctggat cgacgcaccc tcgacgaagt caccggccac gaacacgccg ccgcccaggt
     2461 cgcacggcgg ccgctgcgtg cccggcgtga tcgccggcag gctgtgctgc acctcgtgga
     2521 cggcgagcag ccgccagtcg tcggtcgggc cgtcgtagat cgcgctcgcg tgggcccgca
     2581 cctcaggctc ggacggggcc gcaccctcga gcaccgccga gcattgcacc agatgctggc
     2641 ccgacggggc atagccgggg gccacgttcg acaccaccgc ggtgttcacc aacggcccgt
     2701 gcccgtcggc gtccacgaac agcatcggca gctgcgacgg cgcgacgggg gtgctgaacc
     2761 atcaggtgac caggccgcgc atgggcaccg gcggcagtga cgtgagctcg cccaccgcca
     2821 ccggatcgac ggccaccacc acggcgcggg ccgccagttc ggagccgtcg gcgatcgcga
     2881 cccgcacgcc gtccggtgtg cgtcgcacct ggtcgacgga cgcttccagg cgcacactca
     2941 gaccctccgc cagcaggcgc ggcagcgcgc gcatgccgtc cgagggcaac cccggcgtgc
     3001 cgcgcagaaa ccagccgatc agcgaccgga cgaacgccgc ggacgtcgac cccgtgcgat
     3061 cggccagcac gccgaccaga aactgttcga cggcctcgcg cagcggaccg ctgaaccccg
     3121 cccggtcgag ggacgccgcc agcgtgcggt cgtccgcccg ggtgtccgag ccgcgcagcc
     3181 aggccagcgc gccgcccacc tgtgccggac gcagatagcg gctgccggcc gcacgtaccg
     3241 ccagcaccgg atgccgcacg ggatcggcca gcacggtcag cccgtccaca tcgcgcaccg
     3301 ccaccccgcg ctgaaactgc cgcaggtcga gatcggccac ctgcactgcg tcgcgcagct
     3361 cggcataggc ggggttgatc agctgaaagc cgcggtcgag cagaaagccg tccacctcgt
     3421 cggtggcgac ccggccgccg gcccggtcgg cggcttcgag caccagcacc gaacgtccgg
     3481 cctcggccag cacccgggcg gcgcgcaagc cggccagccc ggctccgatc acgatcacgt
     3541 cggcgtccat gactactcct tcgtgggggt cgttggcgca taggcgccca gcaggccggc
     3601 catcggcacc gaccagacct cttcgccggc gacaccccac tgggccagca gggcgttggc
     3661 ggccaacgtg ccggtggtgg cggcgcgctc catcaacgcg acgggcaggt cgcagcgcac
     3721 ccagtcgccg gccagcacga gtccgggcca cggggtatgg acgcccggac gctccgccca
     3781 cggcgacagg ccgatcagac cgcagtcgtc ctcgaccaac atctcgcgcg ccacgatgcc
     3841 ggccgaggcc gtctcgggcc aggcccggtg cagctcgtcc agcaggcgtt gctcgatgtt
     3901 gttcgcgtcg tacagggcgt agccgtgcag ttcgaccacc gagccaccgg acgccgccgc
     3961 ccagcgtgcc gcgcccgctt cgaagcggtc gagcaccgag atgttgtcga gggggccgaa
     4021 cccgctggtg ccgacgaatg ccggacgctc ggcagccacg ggccgatcca gccacaggcg
     4081 cagcaccgcg aacggcgggg cgttgtgggt ggccgcgacg cgctgcgtcc aggtcgcagt
     4141 cggagtgtcg gtcgcggtgt cgagctcgcc gaccagggtg cgggcagacc gggggtcgag
     4201 ggccagcacg aggccgtccg cagccagttc ggcaccgtcg acgatcaccc ggccggccga
     4261 cggatccagc gactcgaccc gcgcgccggt gcgcacctcg accccgagcc cggccaggta
     4321 cccgcccagc ggcgcccaca gtgcggtgtc gtaatcgtcg tcgggcacgt cgaacagcag
     4381 gccctcggcc gaacccagaa agtaggtgtg gaacatggcc accagctcgc ctgccgagaa
     4441 ctcgcgaggg tcggcgaaga agctgcgggc gaacacctcc agggccaggt cgcgggccgc
     4501 cgcgggaaac cgcagccgat cgagaaaggc agcggccgac tcgccgtcgt agcgcgcgta
     4561 cgagccggga aaacgcacct gtagcagttc cagtgcactg gacacgtcga ccctggtcag
     4621 atcgcgcagc cgaaaggtgg ggctggccag cacgaagccg agaatgctcc acggcggggt
     4681 gcggggcagc gacgtgaacg agtcggtgag gccgtccggc ctggtcagcg ggtagtcctc
     4741 gatcggcacc agacgatcga gcgacgggtc ggtgcggcgc agcagagcgc gcaggttgta
     4801 gtactgccga aagaatgcgt gaaagccgcg gctcatcgtg cgcccgtcgg gcagcggcca
     4861 ggcccggacc cgtccgccga gttgctgctg cgcctccagc accgtcacgc ggacgccccg
     4921 ttcggccagc acggtcgcag cggccagccc ggcgatgccg ccaccgacca cgatcacatg
     4981 ccggggctcg cgcacccgcg cgacgcctcc ctgggccggg tgccggacgg cgtgccggtc
     5041 acgtccgggc agccggcggg gcggacggcc gatcatgacg ggcctcgttc accacgcggt
     5101 tcgcgatcgc cgtggtcgat ggtcgaggcc gcttcgacca gcggcttgcg ggcccggatc
     5161 acatgcagga tgccgcgctg ccagccgggc acggtgcagc tttcgacgtc gacgaaaccg
     5221 accctgtgca gccgggccac gaaccggggg acggtgtcga actcgagcac gctgcgctcc
     5281 aggtaccgat acaggccgtg gttgccgccg gtcagcgtcg cgagcggcag gatcacggcc
     5341 tcgcacacgg cgcgccacac ctgcgccgcc cgcgcatcgc cggccacgga gtactccagc
     5401 accaccagcg ccccacccgg acgcagcgcc gcccacgcgt cggccagcgc cgcatcgcgg
     5461 tctgcctcgg gcacattgcg aaacaggtag gcggcgagaa tgccgtcggc cggagcgtcc
     5521 agcagagggg tcagttcgcc ggcccggccc tgggtgaacg acaccccgcg cggccaggtc
     5581 ttgccccgcg cctgcgccag catgccggcg gacgcgtcca caccctgaat ctcgaccccc
     5641 tcgccgaacg tgctcaccaa cgccctggtg gacgctcccg agccgcaccc caggtcgacc
     5701 agacgggacg ccgccggtac caggtcggcc agggcgtccg ccgcccggcg caggtgccgg
     5761 tggtacccgg ggttcaggcc gaccagaagg tcgtagcggg gtgcacccgc gtcgaactcg
     5821 cgggtcacgt catcgctcgc gtgcgtgagg gcagccgggg ccttcggcgt atcgccacga
     5881 acggcagatc gcacgggagc ctgggccgtc gtgcccccgt tggtggaggc ggcctcgatc
     5941 ttcattcggt ctcctagtgg gtcagccggt gccacaccag cagggtcagg gtgaccatgg
     6001 cgaaaccgaa tccgaagtcc tcgatcggta tgtcgaacgg aaaccggatg ccgctgtact
     6061 ggtcggggta gtagatgacg atcgggtccg acagcttggt cagccagcca tcgacccaga
     6121 tctgaaagaa gaacaccagt gccatcgcca gccagtactg cgcggtgcgc agcaggccgg
     6181 ttcgcaacca gaagaactcc agggcgagca cacccagcac ggcgatcagg gtgaacagcg
     6241 tgtactcacc catcgtcgct gctccggacg ccgtcgttgc tccggacgcc gtcgccgcgt
     6301 tgccgcagcc ggcgcagcac ggtgcccacc gcctcgtagg tgagcaggcc gcagatgggg
     6361 atcacgatga agaagaccag ttcttccaac ggcatcacgc cgaggtggat gccgctgatg
     6421 taggcggggt tgtaggtcca gtgcaggcgg atgatgccga ccacgtccca gatcgagaag
     6481 atcagcacgg tgggcagcag ggccagcagc atcaaccgcc aacgggcgta cacccgggca
     6541 cccagcacga tctcgagcgg cagggtgatc agcaggcagg cggccatcag cagcaggtac
     6601 tgatagtgat ccatgcacac ccccgttcaa ggtttgttca accctagcgg tgagtcgacg
     6661 catacctggc cagctgccgt tgctcgtctc ggatccaccc atctgcgcct gctgcgcggc
     6721 gtgtccgggc accggaacgc accggctcga cccaccggac gccgtcccgc ggcgctggcc
     6781 agacgcctca cggcagcggg tggctccggc cacggcagcc ggggtcgagg aaagagccga
     6841 tcaaccggct gtccgttggc cggcccggtc gaaacccgtc gtggcaacgg tctcgacacg
     6901 ctcgatctcg atggatcagc ctctcctcgg atcacccaca ctgctttgac gtcagtccag
     6961 cacgttgcgc acggaggttc gcggcaggtc gccgaaggcg ttcgacacgc cctggcaggt
     7021 gaccgcgccg cccaccgtgt tcaaccccag cgcgagcgcc tgggactcgt cgcaggcgcg
     7081 acgccacccc ttgtcggcca gctgcacggc gtacggcagc gtggcgttgg tgagccccca
     7141 ggcggacgtg ttgggcaccg cgccgggcat gttcgcgacg cagtagaaca ccgactcgtg
     7201 cacggtgaac gtggggtcgt cgtgggtggt ggggtgtgaa tcctcgaaac aaccgccctg
     7261 gtcgatcgcc acgtcgacca gcaccgagcc cggcttcatg gcggcgacca tgtcgttggt
     7321 gaccagcttg ggcgccttgg ccccgggaat cagcacagtg ccgatcacca ggtcggcggc
     7381 cagcacctgc tcgcgcacgc tcagcgccgt cgacacgatg ccctgaatgc ggttgtcgta
     7441 gcgccagaac gtcatgcgca gcttttcgag gtcggtgtcg agaatggtca cgttggcccc
     7501 catgccgagg gcgatgttgg ccgcgttctg cccggccaca ccgccgccca ggacgaccac
     7561 cttggcgttc ggtacgccgc cgaccgcgcc catcagcacc ccgcgtccgc cctgcgcctt
     7621 catcatcgaa tgtgcgccca cctggggcgc gagacaaccc gccacctcag acatcgggta
     7681 cagcagcggc agcatgccgt tgggcagttg cacggtctcg taggccagcg cggtggtgct
     7741 ggtggtgagc agggcctcgg tcagcggacg gtccgcggcc aggtgcaggt aggtgaacag
     7801 cagcaggtcg tcgcgcaggt agccgtactc ggactcgatc ggctccttga ccttcatcac
     7861 catctcgccc tcggcccagg tggcgtccgc gtcgggcagg atcgtcgccc cctggtcggc
     7921 gtactcctcg tcggtgatgg acgagccgag gccggccccg gcctgcacga acacctgatg
     7981 accacggtga accaactcgt tcaccccgac cggtgtgatc gccacccggt actcgcgatt
     8041 cttcacttcg ctgggaaccg cgattctcat ggcactcctc aaggccggtg tccggggccg
     8101 ggtcgcagcc accagggcgg atggtggctc agagtgtaga gcggacgacg cacgcgcggg
     8161 caggagtcgg cgaacggaag gggcgcggca gccgtcgacg cagggccgtg accgcaaacc
     8221 tcgggtctcc ggttcggtcg gcccgcaccc ctatgctgcc gtccatgctg agctggagcg
     8281 acctggccgc ccgtacgctg gcacgccagt tcccggtcga ggtcgagccc ggcgatgtgc
     8341 agggcctgct ggcaagggtg gggccgatcc agtcgcaaac cgcccggtcg gtgtttctcg
     8401 gcgtggccgc ccggctgccc ggggtcaccc gcgagcaact caccgccgcg tacgagtcgt
     8461 cccagatcgt gcgtggcagt accct
//
LOCUS       DAOSUG010000057         8423 bp    DNA     linear   ENV 10-JUN-2024
DEFINITION  MAG TPA_asm: Micropruina sp. isolate AMR_MDS_2195
            NODE_4385_length_8423_cov_4.372012, whole genome shotgun sequence.
ACCESSION   DAOSUG010000057 DAOSUG010000000
VERSION     DAOSUG010000057.1
DBLINK      BioProject: PRJNA925477
            BioSample: SAMN32864262
            Sequence Read Archive: SRR4236673
KEYWORDS    WGS; ENV; Metagenome Assembled Genome; MAG; Third Party Data; TPA;
            TPA:assembly.
SOURCE      Micropruina sp. (wastewater metagenome)
  ORGANISM  Micropruina sp.
            Bacteria; Bacillati; Actinomycetota; Actinomycetes;
            Propionibacteriales; Nocardioidaceae; Micropruina.
REFERENCE   1  (bases 1 to 8423)
  AUTHORS   Abdulkadir,N., Saraiva,J.P., Zhang,J., Stolte,S., Gillor,O.,
            Harms,H. and Rocha,U.
  TITLE     Genome-centric analyses of 165 metagenomes show that mobile genetic
            elements are crucial for the transmission of antimicrobial
            resistance genes to pathogens in activated sludge and wastewater
  JOURNAL   Microbiol Spectr 12 (3), e0291823 (2024)
   PUBMED   38289113
REFERENCE   2  (bases 1 to 8423)
  AUTHORS   Abdulkadir,N.
  TITLE     Direct Submission
  JOURNAL   Submitted (24-MAR-2023) Environmental Microbiology, Helmholtz
            Center for Environmental Research -UFZ, Permoserstr 15, Leipzig
            04318, Germany
COMMENT     The annotation was added by the NCBI Prokaryotic Genome Annotation
            Pipeline (PGAP). Information about PGAP can be found here:
            https://www.ncbi.nlm.nih.gov/genome/annotation_prok/
            
            ##Genome-Assembly-Data-START##
            Assembly Method        :: SPAdes v. 3.11.1
            Genome Representation  :: Full
            Expected Final Version :: Yes
            Genome Coverage        :: Not Applicable
            Sequencing Technology  :: Illumina MiSeq
            ##Genome-Assembly-Data-END##
            
            ##Genome-Annotation-Data-START##
            Annotation Provider               :: NCBI
            Annotation Date                   :: 05/04/2023 04:57:10
            Annotation Pipeline               :: NCBI Prokaryotic Genome
                                                 Annotation Pipeline (PGAP)
            Annotation Method                 :: Best-placed reference protein
                                                 set; GeneMarkS-2+
            Annotation Software revision      :: 6.5
            Features Annotated                :: Gene; CDS; rRNA; tRNA; ncRNA
            Genes (total)                     :: 2,776
            CDSs (total)                      :: 2,750
            Genes (coding)                    :: 2,714
            CDSs (with protein)               :: 2,714
            Genes (RNA)                       :: 26
            tRNAs                             :: 24
            ncRNAs                            :: 2
            Pseudo Genes (total)              :: 36
            CDSs (without protein)            :: 36
            Pseudo Genes (ambiguous residues) :: 0 of 36
            Pseudo Genes (frameshifted)       :: 2 of 36
            Pseudo Genes (incomplete)         :: 32 of 36
            Pseudo Genes (internal stop)      :: 2 of 36
            ##Genome-Annotation-Data-END##
FEATURES             Location/Qualifiers
     source          1..8423
                     /organism="Micropruina sp."
                     /mol_type="genomic DNA"
                     /submitter_seqid="NODE_4385_length_8423_cov_4.372012"
                     /isolate="AMR_MDS_2195"
                     /isolation_source="Activated sludge of municipal
                     wastewater treatment plants"
                     /db_xref="taxon:2737536"
                     /environmental_sample
                     /geo_loc_name="Canada"
                     /lat_lon="49.25 N 123.1 W"
                     /collection_date="2015-06-16"
                     /metagenome_source="wastewater metagenome"
                     /note="metagenomic"
     gene            <1..190
                     /locus_tag="PLF56_03115"
     CDS             <1..190
                     /locus_tag="PLF56_03115"
                     /inference="COORDINATES: protein motif:HMM:NF024989.2"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=2
                     /transl_table=11
                     /product="zinc-binding dehydrogenase"
                     /protein_id="HOQ52585.1"
                     /translation="TRQRLKPFTSIGKRHDLLTLADLLTTAQVKPVIDRTYRLDEAAD
                     ALRHVADGHTRGKVVVTI"
     gene            275..760
                     /locus_tag="PLF56_03120"
     CDS             275..760
                     /locus_tag="PLF56_03120"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_013864910.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="DUF6326 family protein"
                     /protein_id="HOQ52586.1"
                     /translation="MTIRTNTPKMLHTPPVPVQAKLAAAWTSFMFLYVYVDILNFYKP
                     GVIDGIHNGLVWQFDISPTLLTVFLVSVSIPAAMVILSMTLPARANRATNLVVATLLI
                     PYSIFNAAGASWDWAAFYGISIGLEVLLLGFILRSARRWPRTAPTPTDTAGANPLQVR
                     R"
     gene            1842..2357
                     /locus_tag="PLF56_03125"
     CDS             1842..2357
                     /locus_tag="PLF56_03125"
                     /inference="COORDINATES: protein motif:HMM:NF024835.2"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_function: GO:0003677 - DNA binding [Evidence IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="helix-turn-helix transcriptional regulator"
                     /protein_id="HOQ52587.1"
                     /translation="MNRSEVLRRGMDSTGTSQAELSRLTGLPASKISRYVSGKLEPSE
                     PTLDLLMSSLGLQVGFEVSPVPMERTKRRSWLLHRQISHNLGITGIDDDGWNRMQRNL
                     DRVRSNTQGPVHERNLNRWQQLIDTRNLRNLQRVLVDTSSDGIEMREVAPMTGFLTED
                     ERLGVLAVIQR"
     gene            2354..2500
                     /locus_tag="PLF56_03130"
     CDS             2354..2500
                     /locus_tag="PLF56_03130"
                     /inference="COORDINATES: ab initio
                     prediction:GeneMarkS-2+"
                     /note="Derived by automated computational analysis using
                     gene prediction method: GeneMarkS-2+."
                     /codon_start=1
                     /transl_table=11
                     /product="hypothetical protein"
                     /protein_id="HOQ52588.1"
                     /translation="MNRSQLHHAIRAACAIADVDRVIVVGSQSILGSFDVDELPPVTT
                     FVRR"
     gene            2505..2819
                     /locus_tag="PLF56_03135"
     CDS             2505..2819
                     /locus_tag="PLF56_03135"
                     /inference="COORDINATES: ab initio
                     prediction:GeneMarkS-2+"
                     /note="Derived by automated computational analysis using
                     gene prediction method: GeneMarkS-2+."
                     /codon_start=1
                     /transl_table=11
                     /product="hypothetical protein"
                     /protein_id="HOQ52589.1"
                     /translation="MPDVDDFDRMVKLSDLIEGVAGELSPFEQLHGFALDGVDLTASA
                     LPLGWRDRLVEVRNESTKDPVTGKQYTGCASTPPTCAWRNCARVAKRTAHSSPPCSTP
                     TW"
     gene            3257..3751
                     /locus_tag="PLF56_03140"
     CDS             3257..3751
                     /locus_tag="PLF56_03140"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_017932924.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="SRPBCC family protein"
                     /protein_id="HOQ52590.1"
                     /translation="MSDRTDQPGNGEPGPTVTRVVNAPIDAVWAVLADGWSYPVWVVG
                     ASRVRDVDAGWPAVGTRIHHAFGPWPLVVQDYTRVDDAEPPIDLVMTARGWPVGEARV
                     HLHLEARGEGACEVSITEDATKGPGTLIPSPLRHLVLGPRNRETLYRLALLAEGRHRD
                     ATSS"
     gene            4017..4817
                     /locus_tag="PLF56_03145"
     CDS             4017..4817
                     /locus_tag="PLF56_03145"
                     /inference="COORDINATES: protein motif:HMM:NF014016.2"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_function: GO:0016829 - lyase activity [Evidence IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="CbiX/SirB N-terminal domain-containing protein"
                     /protein_id="HOQ52591.1"
                     /translation="MTAPALVMVGMGSTDAQVAQVALSMRQGLQRMRSGLSVHCAFLE
                     HCSPTPAQVVSQLIKQGVTEIVLVPLQLTHAIDPDERVLGQAVKLRSIHPDLRIQVSR
                     PIGPEVTLLTVLDQRLRSSLGAHRVLELDGLVLSSARTGDVRGNALLARRARQWSTHH
                     RLPCLTAVADGSGPSVSQAIQGLRAQGRRHIAVGSFFLTATELFHAQAELAERCGAVS
                     ISEPLGSAREVMDLILARYAFAAMDLLDVGFDDLEQDEPTRHLSIVSA"
     gene            complement(4897..5040)
                     /locus_tag="PLF56_03150"
     CDS             complement(4897..5040)
                     /locus_tag="PLF56_03150"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_008361769.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="GPGG-motif small membrane protein"
                     /protein_id="HOQ52592.1"
                     /translation="MATVLWIIAAVLVISGVLAIIRKQLLWGIVLIVVGLLVGPGGVS
                     IFH"
     gene            complement(5191..6087)
                     /locus_tag="PLF56_03155"
     CDS             complement(5191..6087)
                     /locus_tag="PLF56_03155"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_002515034.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="polyphosphate kinase 2 family protein"
                     /protein_id="HOQ52593.1"
                     /translation="MAGKNKKKSIAKSGKHASQSLTQLLRLPSGPVDLATLDTAATAG
                     YPGKGKQDAPQLTQALAPEISDLQERLYANGRAAPEQAPSVLVLLQGMDTSGKGGVIR
                     HAMGMVDPQGIQLTSFKAPTDEERKHHYLWRIRRALPDPGMIGIFDRSQYEDVLVVRV
                     EQLVPVSEWNKRYDEINALEKKISDEGTTIIKCFLHVSFAEQKARLAARLESPEKYWK
                     YNPKDIDARAKWPAYQQAYADALERCNTEHAPWFVIPSDRKWYRNWAVAQLLREHLVG
                     LDLGWPEATFDVAAEKTRVAKA"
     gene            complement(6151..7218)
                     /locus_tag="PLF56_03160"
     CDS             complement(6151..7218)
                     /locus_tag="PLF56_03160"
                     /EC_number="6.5.1.1"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_020384782.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_function: GO:0003910 - DNA ligase (ATP) activity
                     [Evidence IEA];
                     GO_function: GO:0005524 - ATP binding [Evidence IEA];
                     GO_process: GO:0006281 - DNA repair [Evidence IEA];
                     GO_process: GO:0006310 - DNA recombination [Evidence IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="ATP-dependent DNA ligase"
                     /protein_id="HOQ52594.1"
                     /translation="MQLPVMPPVKPMLAKSVPTIPTGALSYEPKWDGFRSIIFRDGDE
                     VEIGSRNERPLTRYFPEIVEAVKVNCPPRCVLDGEIVVVVGDRLEFEVLQQRIHPAAS
                     RITMLSEQTPASFVAFDLLALGDDDVTWRPLAERRAALESALDGAQAPIHLTPATRDP
                     AVAQDWFEQFEGAGLDGVVAKPLAGTYQPDKRTMFKIKHQRTADCVVAGYRLHKGTDD
                     AVGSLLLGLYNQQGELASVGVIGAFPLARRKELFAELQPLVTTFDDHPWAWAKQQEGG
                     RTPRNSEFSRWNNGKDLSFTPLRPELVVEVRYEHMEGVRFRHTAQFNRWRPDRDPRSC
                     TYEQLDEPVSYDLAAILALKS"
     gene            7323..7940
                     /locus_tag="PLF56_03165"
     CDS             7323..7940
                     /locus_tag="PLF56_03165"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_013864818.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="cysteine hydrolase"
                     /protein_id="HOQ52595.1"
                     /translation="MPDDALSLDPAKTAVVLIEYQNEFTSEGGVLHGAVDAVMQSTGM
                     LENTKAFVEKARAAGVTIMHAPITFAPGYSELTRHPYGILAGVVGGNAFVKGTWGAAI
                     VDDLAPSEGDILIEGKRGLDTFASTNLDFILRSKGIETVILGGFLTNCCVESTMRSAY
                     ENGYRVITLTDCVAATSPEEHENAIRFNYPMFSHPLTSDEVAAAL"
     gene            complement(8026..>8423)
                     /locus_tag="PLF56_03170"
     CDS             complement(8026..>8423)
                     /locus_tag="PLF56_03170"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_011757096.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=3
                     /transl_table=11
                     /product="ATP-dependent DNA ligase"
                     /protein_id="HOQ52596.1"
                     /translation="QVTVNWWKEERGARIFVDFNQMARDRLMTSAYSIRPTPAGRVSA
                     PLTWDELPSVHPDDFTLLTMPARFADLGDPWAPLYAADPAPISTALEWYAADDVDGGA
                     ELPYPPEYPKMPGEPPRVQPSKKNPANWD"
BASE COUNT         1439 a         2883 c         2655 g         1446 t
ORIGIN      
        1 cacccgacaa cggctgaaac ccttcacctc gatcgggaaa cgccacgacc tgctcaccct
       61 ggccgacctg ctcaccaccg cacaggtcaa gcccgtcatc gaccgcacct accgcctcga
      121 cgaagcagcc gacgcgctcc gccacgtcgc ggacggccac acccgaggga aggtcgtcgt
      181 caccatctga ccaaagcgcc acccaacccg gcgaagtcac tcgcacgcgc cgccccgatc
      241 ggcggaccca ccgctcctcg acaaggaaga acccatgacc atccgcacga acacaccgaa
      301 gatgctccac accccgccgg taccagtcca ggccaaacta gccgccgcct ggaccagctt
      361 catgtttctc tacgtctacg tcgacatcct caacttctac aagcccggcg tcatcgacgg
      421 catccacaac ggcctcgttt ggcagttcga tatcagcccc acgctgttga ccgtcttcct
      481 tgtgtccgtc tcgatccctg cggcgatggt gatcctctcc atgacgctgc ccgcccgagc
      541 gaaccgcgcc acgaacctcg ttgtcgccac gctcctcatc ccctactcga tcttcaacgc
      601 agcaggggcc agctgggatt gggccgcttt ctacggcatc tccatcggcc tggaggtact
      661 gctcctcggg ttcatcctcc gctcggccag acggtggccc cggaccgccc ccaccccgac
      721 agataccgcc ggggccaacc cgctgcaagt ccgtcgctag tcgcaacaag accccgtcga
      781 cgcccccctc gaccccaaca acgccagccg ctacgcctac gccggcaacg accccatcaa
      841 caacaccgac cccaccgggt gtgcagggca aggttgcttc atacgcctgg gatatggccg
      901 gaggcatgct tgggttcggc attggcggaa tcttcgccgg ggggaccgga ggtgcttccc
      961 tgttcgccgg cctgatcttc gccgccgcct ggaaggcgtt cagcgaagga cttggacttg
     1021 aggaacgagc atgccgctaa tcgccgcata cttagtatgt gagagaggcg tccaccgcgc
     1081 catgggacga agagggatca accatgacta atcaaaatga aggggaacgt gccaagcctc
     1141 ctcatcggcg gcaacttctt tgtccgatgg ttgatatggt cactagtatt cgcactaata
     1201 tcaacagccc tggatatcat catccaccgc ggcccttcag ggctatcgtg ggcgtccata
     1261 gtgccgagtg ctctgatcta tggagccttc ttggcagcgc tggcgcaaaa acagttcata
     1321 cagacgaaga tccccgtgaa atggctatac gccgcattca tcgggttcgc aatactcgtc
     1381 acgatcagca cgatcttgct caacgaccgg gtccttaggc ccgcaatcag caccggagcc
     1441 tgggctgtcg cgctcgtcct atgcctggtg gcgcaccgtc ggaaacagga ctagctcggc
     1501 cactggcgga accttggtgc gcccactctc gtcgcgtcca gttcgctctg caacggtctt
     1561 tgcgcccaca cgccgtcgtc acagaggccg agcaagagct tggagaaccc ctgacaccag
     1621 tccgcttcct acagataacg gccacatcaa cgaacaccct ctggaacgct ctaccccgcg
     1681 gcaagaagct cggccgtgac cggcgagact ccctgatcgg ccgagacaag cacagacctt
     1741 gttgaaaccg tctccgaaca tgtgctctcg catgagtgcg caaaggttcc gctggcggga
     1801 cgctgccccg acggcttacc acctggtggt acgctgtttg cgtgaacagg agtgaggtgc
     1861 tgcggcgcgg gatggactcg accggtacca gccaggccga gttgtcccgc ctgacggggc
     1921 tgcccgccag caagatcagc cgctacgtct cgggcaaact cgaaccctcc gagccaaccc
     1981 ttgacctgct gatgtccagc ctcggtctcc aggtcggctt cgaggtgtcc ccggttccga
     2041 tggagcgcac caagcgacgc agctggctgc tgcaccgcca gatctcccac aacctcggca
     2101 tcaccggcat cgacgatgac gggtggaacc gcatgcagcg caacctcgac cgggtgcggt
     2161 cgaacaccca gggccccgtc cacgagcgga acctgaaccg gtggcagcag ctcatcgaca
     2221 cccggaacct gcgcaacctc caacgcgtcc tggtcgacac ctcaagcgat ggcattgaga
     2281 tgcgcgaagt cgcacccatg accggcttcc tgacagagga cgagcggctc ggcgtgctgg
     2341 ccgtgatcca gcggtgaacc ggtcacagct gcatcacgcc atccgcgccg cctgcgccat
     2401 cgccgacgtg gatcgcgtga tcgtggtcgg ttcgcaatcc atcctcggct cgttcgacgt
     2461 cgacgagctt cccccggtca ccaccttcgt ccgaaggtga tgtcatgccc gatgtcgacg
     2521 acttcgaccg catggtcaag ctgtccgacc tgatcgaggg ggtggccggc gaactgtccc
     2581 cgttcgagca gttgcacggc ttcgccctgg acggcgtcga cctcaccgca tccgcgctgc
     2641 cactcggctg gcgtgaccgc ctcgtcgagg tccgcaacga atccaccaag gacccggtca
     2701 cgggcaagca gtacacgggc tgtgcctcga ccccgccgac ctgtgcgtgg cgtaactgtg
     2761 cgcgggtcgc gaaaaggacc gcgcattcgt cgccgccttg ttcaacgccc acctggtagc
     2821 ccgcgacctc gtacgagccc gcctcaacca gctgccggcc cagcgcagcc acaccgcagc
     2881 ggcagcgctc gcggaaactc aacgcgaaag cacagtgact ccaacgcctg tggcggaaag
     2941 tgcgatcccg tgcccgcaag ggttcgcgga gtggatgcgt gacgcctcgc acactctgcg
     3001 cggcatgagc ggaggtgtga tcagactgac gagacggcgg tgaagacggg ctggaagtcc
     3061 tctatgtgag cactccatga gccgttcgaa ggcatgaatg gggcgcacgt cgactctcga
     3121 gcgttttcga cgccgcagtc ttgcccgcag ggttcaacag ccgccgaatg ggtacagtct
     3181 cggcacgggc cgagcgtcgc acaccttcca caccggcgga cgcacccacc cggcgcggac
     3241 gaaaggacga ccgaccatga gcgatcgcac tgaccaaccg ggcaacggcg agcccggccc
     3301 caccgttacc cgcgtcgtga acgcacctat cgatgccgtc tgggcggtac tggccgacgg
     3361 ctggtcctac cccgtctggg tggtcggtgc gtcccgggtg cgcgatgttg atgccggctg
     3421 gcccgcggtc ggcactcgca ttcaccatgc gttcgggccg tggcccctgg tcgtgcagga
     3481 ctacacccgt gtggatgatg ccgagccgcc gattgacctg gtgatgacgg cgcggggttg
     3541 gccggtcggc gaggcgcgcg tgcatctgca cctggaggcc cggggcgaag gtgcgtgtga
     3601 ggtgtcgatc accgaggacg ccacgaaagg acccggcacc ctgattccgt cgccactgcg
     3661 gcacctggtg ttgggccccc gtaaccgtga gacgctgtac cggcttgcgc tgctggcgga
     3721 gggacggcac cgcgacgcca cgtcgagctg acagttcacc gagtcgttcg ggcatcacat
     3781 gccgctcggt gcaccgtcca aggcttgatg ggtcgttccg cttactgaga ttttcaggtt
     3841 gcgcacagcc aatccgtcgc gcagtctcag aaatctggcc gattcatctc attccgtgga
     3901 ctcaatgtcc gcatgatgga tgctcgattt gcatctggta gtggtaacca gaaaggttgg
     3961 tgctacgaaa cgccggcaac ggagccgcag agcatgtgac gcgagagagg cacatcatga
     4021 ccgctccagc cttggtgatg gtgggaatgg gtagcaccga tgcccaagtg gcgcaggtgg
     4081 cgctctcgat gcggcagggt ctgcaacgca tgcgatcggg gctgtcggtg cactgcgcct
     4141 tcctcgagca ctgctccccc acgcccgcgc aggtcgtctc ccagctgatc aagcagggcg
     4201 tgaccgagat cgtcttggtg ccgctgcagc tcacccatgc gatcgatccg gacgagcgcg
     4261 tgctcggcca agcggtcaag cttcgcagca tccatcctga cctgcgcatt caggtgtcgc
     4321 gtccgatcgg ccccgaggtc accctgctca ccgtgctcga ccagcggctg cgctcctcgc
     4381 tcggtgccca ccgggtgctc gagttggacg gcctggtgct ctcgtcggcc cgcaccggcg
     4441 acgtgcgcgg caatgcgctg ctcgcccgcc gtgcacggca gtggtccact caccaccggc
     4501 tgccctgcct gaccgcggtg gcggacgggt cgggcccgtc ggtctcgcag gccattcagg
     4561 gactgcgtgc ccaaggtcgc cggcacatcg cggtcggttc gttctttctc accgcgacgg
     4621 agctgttcca tgcccaggcc gaactggccg aacgttgcgg tgccgtttcc atcagcgagc
     4681 cgctcggttc ggcccgcgag gtgatggact tgatcctggc ccgctacgcg ttcgcggcga
     4741 tggaccttct cgacgtgggc ttcgacgacc tcgagcagga cgagcccacg cgtcacctca
     4801 gcatcgtcag cgcctgaccg cacgggcgat caccgtccga ttgcgcacgc gagcccccgc
     4861 cgcaacggcg ggggctcgcg tcgtgcggaa ctcgactcag tggaagatgc tgacgccgcc
     4921 cgggccgacc aacagcccga cgacgatcag cacgatgccc cagagcaact gcttgcgaat
     4981 gatggccagg actcctgaga tcaccagcac cgcagcgatg atccacaaca ctgtcgccat
     5041 gcgcacacct cctgtttcga cgccccgcgc cacccactcg acggtgaagc agggtgggcc
     5101 ttcgcccgat gggctatgcc tacgccaagc caggtgacct tcgctaccca actaaagggt
     5161 ggctcggcca tgacgcagcc cacccgccgg tcaggccttg gccacccgcg tcttctcggc
     5221 ggccacgtcg aaggtggcct cgggccaacc caggtcgagg ccgaccaggt gctcgcgcag
     5281 tagctgggcc accgcccagt ttcggtacca cttgcgatcg gacgggatca cgaaccacgg
     5341 tgcgtgctcg gtgttgcagc gctccagcgc gtccgcgtag gcctgctgat aggccggcca
     5401 cttggcgcgc gcgtcgatgt ctttgggatt gtacttccag tacttctcgg ggctctcgag
     5461 acgggcggcc aaccgggcct tctgctcggc gaacgacacg tgcaggaagc acttgatgat
     5521 cgtggtgccc tcgtcgctga tcttcttctc gagtgcgttg atctcgtcgt agcgcttgtt
     5581 ccactcgctg accggaacca gttgctccac gcgcaccacc agcacgtcct cgtactggga
     5641 acggtcgaag atgccgatca tgcccggatc gggaagcgcc cgcctgatgc gccacaggta
     5701 gtggtgcttg cgctcctcgt cagtgggtgc cttgaacgag gtgagttgaa tgccctgcgg
     5761 gtcgaccatg cccatcgcat gcctgatcac gccgcccttg ccggaggtgt ccatgccctg
     5821 tagcagcacc agcacgctgg gcgcctgctc gggagcggcg cgtccgttgg cgtagaggcg
     5881 ctcctgcagg tcggagatct cgggcgccag ggcctgcgtc agttgcgggg cgtcctgctt
     5941 gcccttgccc gggtagccgg cggtcgcggc cgtgtcgagc gtggcgagat cgaccggccc
     6001 ggagggcagg cgcagcagtt gcgtcagtga ctggctcgca tgtttgcccg acttggcgat
     6061 ggacttcttc ttgtttttgc ctgccattca cccagtctgg cgcacgcagc cgccgtacgg
     6121 gcggtttgac ctgccgagat ttcaggctga ctacgacttc agtgccagga tggcggccag
     6181 gtcgtagctc accggttcgt ccaactgctc ataggtgcat gagcgcggat cgcgatcggg
     6241 gcgccaccgg ttgaactggg cggtgtgcct gaaccggacg ccctccatat gctcgtagcg
     6301 cacctcgacc accaactccg gacgcagcgg cgtgaacgac aagtccttgc cgttgttcca
     6361 tcggctgaac tcggagttgc ggggggtgcg gccgccctcc tgctgtttgg cccacgccca
     6421 cgggtggtcg tcgaaggtgg tgaccagcgg ctgcagttcg gcgaacaact ccttgcgcct
     6481 ggccaggggg aacgcaccga tcacgccgac ggaggccagc tcgccctgct ggttgtacag
     6541 cccgagcagc aggctgccga cggcgtcgtc ggtgcccttg tgcaaccggt agccggccac
     6601 cacgcaatcg gcggtgcgct ggtgcttgat cttgaacatg gtgcgcttgt ccggctggta
     6661 ggtgccggcc agcggcttgg ccaccacccc gtccagcccg gcgccctcga actgctcgaa
     6721 ccagtcctgg gcgacggcgg ggtcgcgggt ggccggggtc aggtggatcg gcgcctgcgc
     6781 gccgtccaga gccgactcga gggccgcccg gcgctcagcg agcggtcgcc aggtgacgtc
     6841 gtcatcgccg agggccagca ggtcgaaggc gacgaagctg gccggcgtct gctcgctgag
     6901 catcgtgatc cggctcgccg ccggatggat gcgctgctgc agcacctcga actccagccg
     6961 gtcgccgacc accacgacga tctcgccgtc gagcacgcag cgcggcgggc agttgacctt
     7021 gaccgcctcg acgatctcgg gaaaatacct ggtcagcgga cgttcgttgc gcgacccgat
     7081 ctcgacctcg tcgccgtcgc gaaagatgat cgagcggaac ccgtcccact tgggttcgta
     7141 cgagagcgca ccggtgggaa tggtgggcac cgacttggcc agcatcggct tgaccggcgg
     7201 catcacgggt agctgcatgg gtgccattgt gcagtcgatc gcgggcaacg gctcgccgga
     7261 cgcggccagc cgtggttcga tgaggcttgg gggacacaca cactcaacga ggaggaacca
     7321 ccatgcctga tgatgccttg agcctggatc cggcgaagac cgccgtggtg ctgatcgagt
     7381 accagaacga gttcaccagt gagggcggcg tgctgcacgg ggcggtcgac gcggtaatgc
     7441 agagcaccgg catgctcgag aacaccaagg cgttcgtcga gaaggccagg gcggccggcg
     7501 tcacgatcat gcacgcaccg atcaccttcg cgcccgggta ctccgagctc acccgacacc
     7561 cgtacggcat cctggccggc gtggtcggcg gcaacgcgtt cgtcaagggc acctggggcg
     7621 ccgcgatcgt ggacgacctc gcgccgtccg agggcgacat cctgatcgag ggcaagcgcg
     7681 gcctggacac cttcgccagc accaacctcg acttcattct gcgtagcaag ggcatcgaga
     7741 ccgtgattct cggcggcttt ctgaccaact gctgcgtgga gtcgacaatg cgcagcgcct
     7801 acgagaacgg gtatcgggtg atcacgctga ccgactgcgt ggcggccacg tcgcccgagg
     7861 agcacgagaa cgcgatcagg ttcaactacc cgatgttcag ccatccgctc acctcggacg
     7921 aggttgccgc ggccctgtaa ggcggcccgt cagcgccccc ctcactatct ccagcctgcg
     7981 aaggtgcgcg ggcggatgat ggttcggggg gcgctcggtg cctggtcagt cccagttcgc
     8041 cgggttcttc ttcgacggct gcacccgcgg cggctcgccc ggcatcttgg ggtattcggg
     8101 cggatacggc agctcggctc ccccgtccac atcgtcggcg gcgtaccact ccagcgccgt
     8161 gctgatcggg gccgggtcgg ctgcgtagag aggtgcccac gggtcaccca ggtcggcgaa
     8221 ccgggccggc atggtgagca gggtgaagtc gtcggggtga acggacggca gttcgtccca
     8281 ggtcaggggc gccgagaccc gtccggctgg cgtgggccgg atcgaatagg ccgaggtcat
     8341 caaccggtcg cgggccatct ggttgaagtc cacgaagatc cgtgcgccgc gctcctcctt
     8401 ccaccagttc accgtcacct gct
//
LOCUS       DAOSUG010000058         8397 bp    DNA     linear   ENV 10-JUN-2024
DEFINITION  MAG TPA_asm: Micropruina sp. isolate AMR_MDS_2195
            NODE_4399_length_8397_cov_3.870655, whole genome shotgun sequence.
ACCESSION   DAOSUG010000058 DAOSUG010000000
VERSION     DAOSUG010000058.1
DBLINK      BioProject: PRJNA925477
            BioSample: SAMN32864262
            Sequence Read Archive: SRR4236673
KEYWORDS    WGS; ENV; Metagenome Assembled Genome; MAG; Third Party Data; TPA;
            TPA:assembly.
SOURCE      Micropruina sp. (wastewater metagenome)
  ORGANISM  Micropruina sp.
            Bacteria; Bacillati; Actinomycetota; Actinomycetes;
            Propionibacteriales; Nocardioidaceae; Micropruina.
REFERENCE   1  (bases 1 to 8397)
  AUTHORS   Abdulkadir,N., Saraiva,J.P., Zhang,J., Stolte,S., Gillor,O.,
            Harms,H. and Rocha,U.
  TITLE     Genome-centric analyses of 165 metagenomes show that mobile genetic
            elements are crucial for the transmission of antimicrobial
            resistance genes to pathogens in activated sludge and wastewater
  JOURNAL   Microbiol Spectr 12 (3), e0291823 (2024)
   PUBMED   38289113
REFERENCE   2  (bases 1 to 8397)
  AUTHORS   Abdulkadir,N.
  TITLE     Direct Submission
  JOURNAL   Submitted (24-MAR-2023) Environmental Microbiology, Helmholtz
            Center for Environmental Research -UFZ, Permoserstr 15, Leipzig
            04318, Germany
COMMENT     The annotation was added by the NCBI Prokaryotic Genome Annotation
            Pipeline (PGAP). Information about PGAP can be found here:
            https://www.ncbi.nlm.nih.gov/genome/annotation_prok/
            
            ##Genome-Assembly-Data-START##
            Assembly Method        :: SPAdes v. 3.11.1
            Genome Representation  :: Full
            Expected Final Version :: Yes
            Genome Coverage        :: Not Applicable
            Sequencing Technology  :: Illumina MiSeq
            ##Genome-Assembly-Data-END##
            
            ##Genome-Annotation-Data-START##
            Annotation Provider               :: NCBI
            Annotation Date                   :: 05/04/2023 04:57:10
            Annotation Pipeline               :: NCBI Prokaryotic Genome
                                                 Annotation Pipeline (PGAP)
            Annotation Method                 :: Best-placed reference protein
                                                 set; GeneMarkS-2+
            Annotation Software revision      :: 6.5
            Features Annotated                :: Gene; CDS; rRNA; tRNA; ncRNA
            Genes (total)                     :: 2,776
            CDSs (total)                      :: 2,750
            Genes (coding)                    :: 2,714
            CDSs (with protein)               :: 2,714
            Genes (RNA)                       :: 26
            tRNAs                             :: 24
            ncRNAs                            :: 2
            Pseudo Genes (total)              :: 36
            CDSs (without protein)            :: 36
            Pseudo Genes (ambiguous residues) :: 0 of 36
            Pseudo Genes (frameshifted)       :: 2 of 36
            Pseudo Genes (incomplete)         :: 32 of 36
            Pseudo Genes (internal stop)      :: 2 of 36
            ##Genome-Annotation-Data-END##
FEATURES             Location/Qualifiers
     source          1..8397
                     /organism="Micropruina sp."
                     /mol_type="genomic DNA"
                     /submitter_seqid="NODE_4399_length_8397_cov_3.870655"
                     /isolate="AMR_MDS_2195"
                     /isolation_source="Activated sludge of municipal
                     wastewater treatment plants"
                     /db_xref="taxon:2737536"
                     /environmental_sample
                     /geo_loc_name="Canada"
                     /lat_lon="49.25 N 123.1 W"
                     /collection_date="2015-06-16"
                     /metagenome_source="wastewater metagenome"
                     /note="metagenomic"
     gene            <1..468
                     /locus_tag="PLF56_03175"
     CDS             <1..468
                     /locus_tag="PLF56_03175"
                     /EC_number="2.7.8.-"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_013863262.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_component: GO:0005886 - plasma membrane [Evidence IEA];
                     GO_function: GO:0000287 - magnesium ion binding [Evidence
                     IEA];
                     GO_function: GO:0016780 - phosphotransferase activity, for
                     other substituted phosphate groups [Evidence IEA];
                     GO_process: GO:0008654 - phospholipid biosynthetic process
                     [Evidence IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="CDP-alcohol phosphatidyltransferase family
                     protein"
                     /protein_id="HOQ52597.1"
                     /translation="DGYLARKYNQRSKLGQVLDPLADRLYILATLIGLAIREIIPWWL
                     VLLLLARDVMMAALVLPLLRTRGFITLPVHFLGKTATFCLLYAFPMVLLGAGAEPWAL
                     GCRVVGWAFALWGAGLYWCAGLMYVGQTLDVLRRFPPVGKGDRPSTTAAVTGT"
     gene            468..1277
                     /locus_tag="PLF56_03180"
     CDS             468..1277
                     /locus_tag="PLF56_03180"
                     /inference="COORDINATES: protein motif:HMM:NF017734.2"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="DUF881 domain-containing protein"
                     /protein_id="HOQ52598.1"
                     /translation="MSAPQARPVRARRVDESMNLLTDLSRDALEPSYRTASPRRRRWW
                     VVVVATAVIGLMIGGSIGDVLRAAPAEQVEREQLVQRIKDASVATDGLRARIATLTAE
                     NRALSDAALANDPAAAEVQRQVEALEPTTGVRAVTGPGVVLVADDSSTTDQSGSQVVD
                     VDIRQAVNGLWLAGAEAISVNGHRLSSRTAVRGAGNAITVDYRSLSRPYRIEAIGDSQ
                     AMLRAFPSGPGGVWWAYLKQNYGIRYDLTSADSLRLDADPGLGVRVAERIR"
     gene            1290..1622
                     /locus_tag="PLF56_03185"
     CDS             1290..1622
                     /locus_tag="PLF56_03185"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_002513635.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="small basic family protein"
                     /protein_id="HOQ52599.1"
                     /translation="MFAIVGLILGIALGIYLEPTLPVGLQPYLPIAIVAALDALFGGF
                     RAYLERVFDDRVFVVSFISNVLIAALIVWVGDQIGVGAQLSTAVVVVLGIRIFTNAAA
                     IRRALFHA"
     gene            1615..2496
                     /locus_tag="PLF56_03190"
     CDS             1615..2496
                     /locus_tag="PLF56_03190"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_013161130.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="DUF881 domain-containing protein"
                     /protein_id="HOQ52600.1"
                     /translation="MPEPDEREPRPAADEGVGSDPINSAVGPDDGSPIAPEQSRAEAD
                     GVSDPSEARRPPIGWRELVRDFVKPGRAQVVFAAILLVLGLGVAMQVRTRAEETDYST
                     LRRDDLVQMLDDLNTESRRLETEIAQLEETKRQLQGGVDRERVARDEAQRRLDVLAIL
                     GGTAPAQGPGIRITITDPNRKVTSDLLLNTVEELRDAGAEVIAINNTVRVVASTWFGS
                     SNAGLVVDNQLVTTPITLDVIGEPHALTESTRFSGGLVSEVQGTRIGGSVSVVTDERL
                     TISAVITPTPNKFAKPA"
     gene            2605..2985
                     /gene="gcvH"
                     /locus_tag="PLF56_03195"
     CDS             2605..2985
                     /gene="gcvH"
                     /locus_tag="PLF56_03195"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_016667310.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_function: GO:0003824 - catalytic activity [Evidence
                     IEA];
                     GO_process: GO:0019464 - glycine decarboxylation via
                     glycine cleavage system [Evidence IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="glycine cleavage system protein GcvH"
                     /protein_id="HOQ52601.1"
                     /translation="MFPEDLKYTDKHEWVRSGNGSIVRIGITSYAADALGDVVYVSLP
                     QVGEEVAKDDACLEVESTKSVSDVFSPASGVITSVNDLLNTTPEVINADPYGDGWMFE
                     LEVSDPSELDDTLDADSYAELTGE"
     gene            3069..3563
                     /locus_tag="PLF56_03200"
     CDS             3069..3563
                     /locus_tag="PLF56_03200"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_002548819.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_function: GO:0005515 - protein binding [Evidence IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="FHA domain-containing protein"
                     /protein_id="HOQ52602.1"
                     /translation="MIKCPACGHENADTNNFCANCGHPLNAQETTGDTTRVVAAVDSP
                     DDTAGEDLSAEDVSAINQLRPNSALLIVRQGPAGGARFLLNSDVTTAGRHPRCDIFLD
                     DITVSRNHARFTRRDGHIWVEDENSLNGSYVNRTLIDGPVALRRGDEVQIGKFRMLFF
                     TQPG"
     gene            3570..4271
                     /locus_tag="PLF56_03205"
     CDS             3570..4271
                     /locus_tag="PLF56_03205"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_013863268.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_function: GO:0003677 - DNA binding [Evidence IEA];
                     GO_process: GO:0006355 - regulation of DNA-templated
                     transcription [Evidence IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="MerR family transcriptional regulator"
                     /protein_id="HOQ52603.1"
                     /translation="MAYGLRSIGQVLSVLKADFPDVSISKIRFLESEGLIAPERAPSG
                     YRRYAESDIVRLRYILTVQKSHYLPLKVIKEHLEAMDRGEDPPRLEPLTGAAAATEVP
                     LPTAAGGQSTARTPKRPIRVTRRELLELSGLSEASLSELERHALVSPRRGTVYYGRDA
                     LTVAVAARKLSAYGMDARHLRVVKQAAEREVGLIEQAIAPHLRRSQSPQQLAQEVMQL
                     VMHAHAAIMRSTMPQ"
     gene            4279..4764
                     /locus_tag="PLF56_03210"
     CDS             4279..4764
                     /locus_tag="PLF56_03210"
                     /inference="COORDINATES: protein motif:HMM:NF014618.2"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="bifunctional nuclease family protein"
                     /protein_id="HOQ52604.1"
                     /translation="MRELNVVEVRLDDEQGSPVLILKESDGERQVAIWMSAAGAAGIL
                     GALQEADPDHPSSHELILALIASLDGEVSVAHIVGHQNGVFYAELIVDGRPVNARPSD
                     AIAVALRAGVPIKIAEAVLDAVGVVPTPPAADDDDDEPEGEDQVEQFRQFLDSVNPDD
                     F"
     gene            4927..5502
                     /locus_tag="PLF56_03215"
     CDS             4927..5502
                     /locus_tag="PLF56_03215"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_008359558.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_function: GO:0003677 - DNA binding [Evidence IEA];
                     GO_process: GO:0006355 - regulation of DNA-templated
                     transcription [Evidence IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="MerR family transcriptional regulator"
                     /protein_id="HOQ52605.1"
                     /translation="MNGLDEAADQPVDTQDLLFDGDFSPLPDDLGFRGPVACGAAEIT
                     YRQLDYWARTGLVVPEVRSAGGSGTQRLYSFRDILLLKVIKRLIDAGISLQQIRTAID
                     HLRARGVQDLTQVTLMSDGVSVYECTSDDEVIDLLRGGQGMFGIALGGVWRDIEGSLA
                     TLPSERVHEEAAPAAAASDELSRRRHARAAG"
     gene            5871..>7285
                     /locus_tag="PLF56_03220"
     CDS             5871..>7285
                     /locus_tag="PLF56_03220"
                     /EC_number="1.4.4.2"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_007077729.1"
                     /note="acts in conjunction with GvcH to form
                     H-protein-S-aminomethyldihydrolipoyllysine from glycine;
                     Derived by automated computational analysis using gene
                     prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="glycine dehydrogenase
                     (aminomethyl-transferring)"
                     /protein_id="HOQ52606.1"
                     /translation="MTAFTTRHLGAVGDDRSQMLTLVGYESLDQLSAAAVPADIALHE
                     PLDLPPALSEAEAAADLAAMAAANRPRRAMIGLGYHGTITPPVIRRNVLENPAWYTAY
                     TPYQPEISQGRLEALLNFQTMISDLTALPTAGASLLDEGTAVAEAMALARRITRKGSV
                     LLVDGAVLPQTLAVVRTRALATGIDVVVIEGDLVEALQTHEAFAVVVQVPGANGHLRT
                     AAELRAIAATAHDNNASVIAAADLLALTLVTAPGDWGADIAVGTTQRFGVPLFYGGPH
                     AAYLAVREGLERQLPGRLVGLSVDVDGTPALRLALQTREQHIRRDRATSNICTAQVLL
                     AVVASMYAVYHGPHGLAGIAERVHHQARRLAAALSARAELTSTSFFDTLTLRVPGEAA
                     GIVEAAAAGGVHLRLVDADHVGVSVGEDTTGADLNAVAAAFGVEPLTEATMGDLAGHR
                     RPVDYLSHPVFNTRHSETEMLR"
     assembly_gap    7286..7295
                     /estimated_length=10
                     /gap_type="within scaffold"
                     /linkage_evidence="paired-ends"
     gene            <7296..>8397
                     /locus_tag="PLF56_03225"
     CDS             <7296..>8397
                     /locus_tag="PLF56_03225"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_013863272.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=3
                     /transl_table=11
                     /product="aminotransferase class V-fold PLP-dependent
                     enzyme"
                     /protein_id="HOQ52607.1"
                     /translation="YLRALADRDFALDRGMIPLGSCTMKLNPTTSMEAISLPGFANLH
                     PFAPASDAQGYLLMIERLCGWLAEITGYDRVSVQPNAGSQGEFAGLMAIRGYHRANGE
                     AQRTVCLIPASAHGTNAASAVMSGMSVVVVKTAPDGSVDLADLRAKVEAHADTLAAIM
                     VTYPSTHGVYENTITELCELVHAAGGQVYVDGANLNALLGLARPGRFGADVSHLNLHK
                     TFAIPHGGGGPGVGPVAVRSHLAPYLPSHPLSDEAGPATSYGPVSAAPFGSAGVLPIS
                     FAFIAMLGADGLKQSGQSAVLAANYVAARIGEHYPILYTGANGLVAHECIADLRPLTK
                     ATGVTVDDVAKRLIDYGFHAPTMSFPVAGTLM"
BASE COUNT         1334 a         2878 c         2822 g         1353 t
ORIGIN      
        1 gatggatacc tggcccgcaa gtacaaccag cgctccaagc tcgggcaggt gctcgatccg
       61 ctggccgatc ggctctacat tctggccacc ctgatcggcc tggccatccg cgagatcatt
      121 ccctggtggc tggtgctgct gctgctcgcc cgcgacgtga tgatggctgc tctggtgctg
      181 cccctgctgc gtacccgcgg gttcatcacc ctgccggtgc attttctggg caagaccgcg
      241 acgttctgcc tgctctacgc ctttcccatg gtgcttctcg gagccggcgc cgaaccttgg
      301 gcgctcggat gccgggtggt cgggtgggcc ttcgccctgt ggggggccgg gctgtactgg
      361 tgcgccgggc tgatgtacgt cggccagacc ctggacgtgc tgcgccggtt tccgccggtc
      421 ggcaagggcg atcgtccctc gaccacagcc gcggtcaccg gtacgtgatg agcgcccccc
      481 aggcccggcc ggtccgtgct cgtcgggtgg acgagagcat gaacctgctc accgatctgt
      541 cccgggacgc actcgagccg tcctatcgca ccgcgtcgcc gcgccggcgc cgctggtggg
      601 tggtggtggt ggccacggcg gtgatcggcc tgatgatcgg tggttcgatc ggcgacgtgc
      661 tgcgggccgc gcccgccgaa caggtggagc gcgaacagct cgtccagcgc atcaaggacg
      721 cgagcgtcgc caccgacggg ctgcgtgccc ggatcgctac gctcaccgcc gagaaccggg
      781 ccctctcgga cgccgcgctc gccaacgacc cggccgccgc ggaggtccaa cggcaggtgg
      841 aggctctcga gccgaccacg ggcgtccgcg ccgtcaccgg tcccggtgtg gtgctcgtcg
      901 ccgacgattc gtccaccacc gaccaatccg gcagtcaggt ggtcgacgtc gacatccggc
      961 aggccgtgaa cggactgtgg ctggcgggtg ccgaggcgat ctcggtgaac ggacaccgac
     1021 tctcgtcgcg caccgccgtt cgtggcgccg gcaatgcgat cacggtggat taccgctcgc
     1081 tcagcaggcc gtaccggatc gaggcgatcg gcgacagcca ggccatgctg cgcgcgttcc
     1141 cgtccggccc ggggggtgtg tggtgggcct acctcaaaca gaactacggc attcggtacg
     1201 acctcacctc ggcggatagt ctgcggctgg acgccgaccc cgggctgggc gtccgggtcg
     1261 ccgagagaat tcgctgagga ggtgccgcca tgttcgcgat cgtgggcctg attctgggca
     1321 tcgccctcgg catctacctc gaaccgacgc ttccggtcgg cctgcagccg tacctgccga
     1381 tcgccatcgt cgcggccttg gatgcactgt tcggcgggtt tcgcgcctac ctggagcggg
     1441 ttttcgatga ccgggtgttc gtggtgtcgt tcatctccaa cgtactgatc gccgccctga
     1501 tcgtgtgggt gggtgaccag atcggcgtcg gcgcgcagct ctcaaccgcc gtggtggtcg
     1561 tgctgggcat tcgcatcttc accaacgccg ccgccattcg acgggccctg ttccatgcct
     1621 gagccggacg agcgcgaacc ccgccccgct gccgacgaag gggtcgggtc cgaccccatc
     1681 aactccgcag tcggacccga cgacggttcc ccgattgcgc ctgagcagtc gagagccgag
     1741 gccgacggag tctccgaccc gagcgaggcc cggcgtccgc cgatcggctg gcgcgagctc
     1801 gtccgcgact ttgtgaagcc tggacgggcg caggtggtct tcgccgcgat tctgctggtg
     1861 ttagggctcg gcgtggcgat gcaggtgcgc acccgggccg aggaaaccga ctactcgacg
     1921 ttgcggcgcg acgacctggt gcagatgctg gacgacctga acaccgagag ccgacgcctc
     1981 gaaaccgaga tcgcccaact ggaagagacc aaacgccagc tgcagggcgg cgtcgatcgt
     2041 gagcgggtgg cccgcgacga ggcacagcgg cggctggacg tgctcgccat cctgggcggc
     2101 acggcgcccg cgcagggtcc cggcattcgg atcacgatca ccgaccccaa tcgcaaggtc
     2161 acctccgatc tactgctgaa caccgtcgag gagttgcgcg acgcgggcgc cgaggtgatc
     2221 gcgatcaaca acaccgtccg cgtggtggcc tcgacgtggt tcggctcgag caacgcgggc
     2281 ctggtggtcg acaaccaact ggtcacgacg ccgatcaccc tcgacgtgat cggcgagccg
     2341 cacgcactca ccgagtcgac gcgcttctcg ggcgggctgg tcagcgaggt gcagggcacg
     2401 cggatcggtg ggtcggtgag cgtcgtgacc gacgaaaggc tcacgatcag cgcggtcatc
     2461 acaccgaccc ccaacaagtt cgccaaaccg gcctgaaccc gggccgtccc gatgcaatcc
     2521 ggtcgtgagg acggcgtgaa gtgagtagtg tgtgcgcagg atgaacgaca tgtccccacc
     2581 cttccgcggc gccgaggagt ccacgtgttt cccgaggatc tgaaatacac cgacaagcac
     2641 gaatgggtgc gcagcggcaa cggctcgatc gtcaggatcg gcatcacgtc gtatgctgcg
     2701 gacgccctcg gtgacgttgt gtacgtgtcg ctgccccagg tcggcgaaga ggtggcgaaa
     2761 gacgacgcct gcctcgaggt cgagtcgacc aagagcgtct cggacgtgtt ctcgccggcc
     2821 tccggggtga tcacctcggt caacgacttg ctcaacacca caccagaggt gatcaacgcc
     2881 gacccctacg gcgacggctg gatgttcgaa ctcgaggtca gtgatccgtc cgaactcgac
     2941 gacaccctgg acgccgactc ctacgccgag ctgacggggg agtagcctca ccgtctacct
     3001 ctggcttagc cttacccctt gatccccttt gcatgtgaaa gagcgttcgc tgcagaaagg
     3061 cctggacgat gatcaagtgc cccgcctgcg gacacgaaaa cgccgacacg aacaacttct
     3121 gcgccaactg cggtcatccg ctgaacgcac aagagaccac cggtgacacc acccgggtgg
     3181 tcgcagccgt cgacagcccc gacgacaccg cgggcgaaga cctcagcgcc gaggacgtgt
     3241 cggcgatcaa ccagttgcgc cccaactcgg cgctgttgat cgtgcgccag ggcccggccg
     3301 ggggcgcacg cttcctgctg aactcagacg tcaccacggc gggtcggcat ccgcgctgtg
     3361 acatctttct cgacgacatc acggtctcgc gaaaccatgc ccggttcacc cgccgcgacg
     3421 gccacatctg ggtcgaggac gagaacagcc tcaacggcag ctacgtgaac cgcaccctga
     3481 tcgacggccc cgtggcgctg cgccgaggcg acgaggtgca gatcggcaag ttccgcatgc
     3541 tgttcttcac ccaacccggg tgacggcgta tggcttacgg cttgcgcagc atcgggcagg
     3601 ttctctccgt cctcaaggcg gactttccag atgtctccat ctccaagatc cgctttctgg
     3661 aaagtgaagg gctgatcgct cctgagcggg ccccgtccgg gtaccggcgg tacgccgaat
     3721 ccgacatcgt ccggctgcgc tacatcctga ccgtgcagaa gtcgcactac ctgccgttga
     3781 aggtgatcaa agagcacctc gaggccatgg accgtggcga ggacccgccc aggctcgagc
     3841 cgttgaccgg cgcagcggcc gccaccgagg tgccgttgcc caccgcagcg ggtggccaga
     3901 gcaccgctcg cacccccaaa cgtccgatcc gggtcacccg ccgcgagttg ctcgaactca
     3961 gcgggctgtc cgaagccagc ctgtccgaac tcgaacgaca cgcgctggtg agtccccggc
     4021 ggggcaccgt ctactacgga cgcgacgccc tcaccgttgc cgtcgcggcc cgcaaactct
     4081 cggcctacgg catggacgcc cgccatctgc gggtggtcaa gcaggcggct gagcgcgagg
     4141 tcgggctgat cgaacaggcg atcgctccgc acctgcgacg cagccagtcc ccgcagcaac
     4201 tcgctcagga ggtcatgcag ctggtcatgc acgcccacgc cgcgatcatg cgatcgacga
     4261 tgccccagta gagccaccat gcgtgaactc aacgtggtcg aggtccgact ggacgacgag
     4321 cagggctcgc cggtgctgat cctgaaggaa tccgacgggg agcggcaggt ggccatctgg
     4381 atgagcgcgg caggcgcggc gggcattctc ggcgcgttgc aggaggccga tcccgaccac
     4441 ccgtccagtc atgaactgat cctggcgctg atcgccagcc tcgacggtga ggtgtcggtc
     4501 gcgcacatcg tcggccatca gaacggggtg ttctacgccg agttgatcgt cgacgggcgc
     4561 ccggtcaacg cgcgtccctc ggacgcgatc gccgtcgcgc tgcgtgcggg cgtgccgatc
     4621 aagatcgccg aggcggtgct ggacgctgtc ggtgtggttc ccacgccgcc cgccgcggac
     4681 gacgatgacg acgaacccga gggtgaggat caggtcgaac agttccggca gtttctcgat
     4741 tcggtgaacc cggacgactt ctgaatcggc tcacctgcaa ccctgaggta cagctggagg
     4801 gttggtaggc tgtcggccac tgccgcggcg tgtcgttgac cgtcgaacac cgccggatct
     4861 agcgtcgtgg aggtcagtac tccggcgcgg ccggggcaca accacgaggc gacgaaggcg
     4921 ggcgacgtga acggtctgga cgaggcggcg gatcagcccg tggataccca ggacctgctc
     4981 ttcgacggcg acttctcgcc gttgccggat gacttgggct ttcgcggccc tgtcgcgtgc
     5041 ggcgcggccg agatcaccta ccggcaactg gactattggg cccgcaccgg actggtcgtg
     5101 cccgaggtgc gttcggccgg cggttcgggc acgcagcggc tgtacagctt tcgagacatt
     5161 ctgttgctca aggtgatcaa gcgcctgatc gatgccggca tctcgttgca gcagattcgc
     5221 accgccatcg atcatctgcg ggcccgaggg gtgcaggacc tcacgcaggt caccctgatg
     5281 agcgacgggg tctcggtgta cgaatgcacc tcggacgatg aggtcatcga cctgctccgc
     5341 ggcgggcagg gcatgttcgg catcgcgctg ggcggcgtct ggcgcgacat cgagggcagc
     5401 ctggccacat tgcccagcga gcgagtgcac gaagaggcgg cacccgctgc cgccgcctcg
     5461 gacgagttgt cccgtcgccg ccacgcccgg gctgccggct gacgcctcaa tcgctctcga
     5521 tctgatggtc gagactgtct gttggtcgag actgtccagc ccgttggccg agcgtcgaga
     5581 cccggggtgt gcggtcgatc cgattctgaa atcacccgcc acggtgtcgg cagtgcccgg
     5641 cgtgtcgcgg gtcggcgggg cgactggtgt caagctttca gaaagtcggc tcgctggcca
     5701 gcgcgtcggg cggatgccgc tcgaggccga cgaggaagtc atgacggctc gaatggtggc
     5761 ccaggcgaac ccagccaggc gccgtccgca cgtgcgcggg cgttgcctga gcgctgagat
     5821 aggttgtgac ggacgctcac gaggcgtccg gaaccacaac aggagtgctc atgactgcct
     5881 tcaccactcg ccatctcgga gcggtcggcg atgatcgatc acagatgctc acgttggtcg
     5941 gttatgagtc gctcgaccag ttgagcgccg cagcggtgcc cgctgacatc gccttgcacg
     6001 agccactcga cctgccgccc gcgctgagcg aggccgaggc agccgccgac ctggccgcca
     6061 tggccgcggc caaccgtccg cgcagggcca tgatcgggct gggctatcac ggcaccatca
     6121 ccccgccggt gatccgccgc aatgtgctcg aaaatccggc ttggtacacc gcctacaccc
     6181 cttaccagcc cgagatcagc cagggccggc tcgaggcgct gctcaacttt cagaccatga
     6241 tcagcgacct cactgcgtta cccaccgccg gggcgagcct gctcgatgag ggcaccgccg
     6301 ttgccgaggc catggcgctg gcccgccgga tcacccgcaa gggttcggtg cttctcgtgg
     6361 acggcgccgt gctgccgcag accctcgcgg tggtgcgcac ccgcgcactg gccaccggca
     6421 tcgatgtggt ggtgatcgag ggtgacctgg tcgaggccct gcagacgcat gaggccttcg
     6481 ccgtggtcgt ccaggtaccc ggtgcgaacg gccatctgcg caccgctgcc gaactgcgcg
     6541 ccatcgcggc caccgcgcac gacaacaatg cctcggtcat cgcggccgcc gacctactcg
     6601 cgctgaccct ggtcaccgcg cccggcgact ggggtgccga catagcggtc ggcaccaccc
     6661 aacgcttcgg cgtgccgctg ttctacggcg gcccgcacgc ggcctacctg gcggtgcgcg
     6721 aggggctgga gcggcagttg cccggacgcc tggtcggcct ctccgtggac gtcgacggca
     6781 ctccggcgct gcggttggcg ttgcagaccc gcgagcagca cattcgtcgg gatcgggcca
     6841 cgtccaacat ctgcaccgcg caggtgctgt tggccgtcgt ggcgtccatg tatgcggtct
     6901 accacggccc gcacgggctg gcaggcatcg cggagcgggt gcatcatcag gcccgacggc
     6961 tcgcggccgc gctgagcgcg cgcgccgaac tgaccagcac gagcttcttc gacaccctga
     7021 ccctgcgcgt gccgggcgag gcggcgggaa tcgtcgaggc ggccgcggcg ggtggcgtgc
     7081 acctgcggct ggtggacgcc gaccacgtcg gcgtgagcgt cggcgaagac accaccgggg
     7141 cagatctgaa cgccgtcgcc gccgccttcg gcgtcgagcc actgaccgag gccacaatgg
     7201 gcgatctcgc cggccaccgg cgtccggtcg actacctgag ccatccggtg ttcaacaccc
     7261 ggcacagcga aaccgaaatg ctgcgnnnnn nnnnngctac ctgcgggccc tggccgaccg
     7321 cgatttcgcg ctggatcgcg gcatgattcc gctcggatcc tgcaccatga agctgaaccc
     7381 caccacctcg atggaggcga tcagcctgcc gggcttcgcc aacctgcacc cgttcgcgcc
     7441 ggcgtccgat gcgcagggct acctgctcat gatcgagcgc ctctgcggct ggctggccga
     7501 gatcactggc tacgaccggg tctcggtgca gcccaacgcc ggtagtcagg gcgagttcgc
     7561 cgggctgatg gcgattcgtg gctaccaccg cgccaacggt gaggcgcagc gcaccgtctg
     7621 cctgattccg gccagcgcgc acggcaccaa tgcggcctcc gcggtgatgt cgggcatgag
     7681 cgtggtcgtg gtcaagactg cgccggacgg ttccgtcgac ctcgccgacc tgcgggccaa
     7741 ggtcgaggcg cacgccgaca ccctggccgc gatcatggtc acctacccat ccacccacgg
     7801 ggtgtacgag aacaccatca ccgagctgtg cgagctggtg cacgcggccg gcgggcaggt
     7861 gtacgtcgac ggcgccaacc tcaatgccct gctcggcctg gcccggcccg gccgcttcgg
     7921 tgcagacgtc agccatctca acctgcacaa gaccttcgcg atcccgcacg gtgggggtgg
     7981 accgggtgtc gggccggtgg ccgtccgctc acatctggcc ccatacctgc cgagccatcc
     8041 gctgtcggac gaagccggcc cggccacgtc gtacggaccg gtcagcgcgg cgccgttcgg
     8101 ctcggcgggg gtgctgccga tcagctttgc gttcatcgcc atgctgggcg cggacgggct
     8161 gaagcagtcg ggccagtcgg cggtgttggc ggccaactac gtcgccgcac gcatcggcga
     8221 gcactatccg attctgtaca ccggtgcgaa cggcctggtc gcgcacgagt gcatcgccga
     8281 cctgcggccg ctcaccaagg cgaccggggt gaccgtcgac gacgtcgcca aacggctgat
     8341 cgactacggc tttcacgcgc ccacgatgag ttttccggtg gccggcacgc tgatgat
//
LOCUS       DAOSUG010000059         8210 bp    DNA     linear   ENV 10-JUN-2024
DEFINITION  MAG TPA_asm: Micropruina sp. isolate AMR_MDS_2195
            NODE_4481_length_8210_cov_4.407480, whole genome shotgun sequence.
ACCESSION   DAOSUG010000059 DAOSUG010000000
VERSION     DAOSUG010000059.1
DBLINK      BioProject: PRJNA925477
            BioSample: SAMN32864262
            Sequence Read Archive: SRR4236673
KEYWORDS    WGS; ENV; Metagenome Assembled Genome; MAG; Third Party Data; TPA;
            TPA:assembly.
SOURCE      Micropruina sp. (wastewater metagenome)
  ORGANISM  Micropruina sp.
            Bacteria; Bacillati; Actinomycetota; Actinomycetes;
            Propionibacteriales; Nocardioidaceae; Micropruina.
REFERENCE   1  (bases 1 to 8210)
  AUTHORS   Abdulkadir,N., Saraiva,J.P., Zhang,J., Stolte,S., Gillor,O.,
            Harms,H. and Rocha,U.
  TITLE     Genome-centric analyses of 165 metagenomes show that mobile genetic
            elements are crucial for the transmission of antimicrobial
            resistance genes to pathogens in activated sludge and wastewater
  JOURNAL   Microbiol Spectr 12 (3), e0291823 (2024)
   PUBMED   38289113
REFERENCE   2  (bases 1 to 8210)
  AUTHORS   Abdulkadir,N.
  TITLE     Direct Submission
  JOURNAL   Submitted (24-MAR-2023) Environmental Microbiology, Helmholtz
            Center for Environmental Research -UFZ, Permoserstr 15, Leipzig
            04318, Germany
COMMENT     The annotation was added by the NCBI Prokaryotic Genome Annotation
            Pipeline (PGAP). Information about PGAP can be found here:
            https://www.ncbi.nlm.nih.gov/genome/annotation_prok/
            
            ##Genome-Assembly-Data-START##
            Assembly Method        :: SPAdes v. 3.11.1
            Genome Representation  :: Full
            Expected Final Version :: Yes
            Genome Coverage        :: Not Applicable
            Sequencing Technology  :: Illumina MiSeq
            ##Genome-Assembly-Data-END##
            
            ##Genome-Annotation-Data-START##
            Annotation Provider               :: NCBI
            Annotation Date                   :: 05/04/2023 04:57:10
            Annotation Pipeline               :: NCBI Prokaryotic Genome
                                                 Annotation Pipeline (PGAP)
            Annotation Method                 :: Best-placed reference protein
                                                 set; GeneMarkS-2+
            Annotation Software revision      :: 6.5
            Features Annotated                :: Gene; CDS; rRNA; tRNA; ncRNA
            Genes (total)                     :: 2,776
            CDSs (total)                      :: 2,750
            Genes (coding)                    :: 2,714
            CDSs (with protein)               :: 2,714
            Genes (RNA)                       :: 26
            tRNAs                             :: 24
            ncRNAs                            :: 2
            Pseudo Genes (total)              :: 36
            CDSs (without protein)            :: 36
            Pseudo Genes (ambiguous residues) :: 0 of 36
            Pseudo Genes (frameshifted)       :: 2 of 36
            Pseudo Genes (incomplete)         :: 32 of 36
            Pseudo Genes (internal stop)      :: 2 of 36
            ##Genome-Annotation-Data-END##
FEATURES             Location/Qualifiers
     source          1..8210
                     /organism="Micropruina sp."
                     /mol_type="genomic DNA"
                     /submitter_seqid="NODE_4481_length_8210_cov_4.407480"
                     /isolate="AMR_MDS_2195"
                     /isolation_source="Activated sludge of municipal
                     wastewater treatment plants"
                     /db_xref="taxon:2737536"
                     /environmental_sample
                     /geo_loc_name="Canada"
                     /lat_lon="49.25 N 123.1 W"
                     /collection_date="2015-06-16"
                     /metagenome_source="wastewater metagenome"
                     /note="metagenomic"
     gene            292..1482
                     /gene="manA"
                     /locus_tag="PLF56_03230"
     CDS             292..1482
                     /gene="manA"
                     /locus_tag="PLF56_03230"
                     /EC_number="5.3.1.8"
                     /inference="COORDINATES: protein motif:HMM:TIGR00218.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_function: GO:0004476 - mannose-6-phosphate isomerase
                     activity [Evidence IEA];
                     GO_process: GO:0005975 - carbohydrate metabolic process
                     [Evidence IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="mannose-6-phosphate isomerase, class I"
                     /protein_id="HOQ52608.1"
                     /translation="MQPLSGTVQRYDWGTHNAIPAILGRPADGRPVAEYWLGAHSSSP
                     SLVGNGTLVDHIGSTPAVLGARSRAAFGEQLPFLMKLLSARHALSIQAHPSREQAIEG
                     FARESRAGTALDAPERTYKDSWPKPEILIALDEFHSLAGFRDPHETAELFAALGVAEQ
                     LDSVIGPLTERKGPAAMAEVFLDVLSLDGSRAHLVDETVSAAMHHRYDDGTVGEFART
                     ALELDAVFPADPGILAALLMNRLVLRPGEALFVPAGMMHAHLHGTGVEVMANSDNVIR
                     GGLTNKHIDVSELVAVVDFNPWLPERLHPRRTRPGVLKYQTPCSEFEAWRMLIDRSTG
                     PVRVPGRGSARILFAIDGACVLDNGEATLQLPRGRAAFLSADELDVFATGTAEVYGAS
                     SGIR"
     gene            1655..1727
                     /locus_tag="PLF56_03235"
     tRNA            1655..1727
                     /locus_tag="PLF56_03235"
                     /product="tRNA-Thr"
                     /inference="COORDINATES: profile:tRNAscan-SE:2.0.12"
                     /note="Derived by automated computational analysis using
                     gene prediction method: tRNAscan-SE."
                     /anticodon=(pos:1688..1690,aa:Thr,seq:tgt)
     gene            1729..2343
                     /locus_tag="PLF56_03240"
     CDS             1729..2343
                     /locus_tag="PLF56_03240"
                     /EC_number="2.1.-.-"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_016669560.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_function: GO:0008168 - methyltransferase activity
                     [Evidence IEA];
                     GO_function: GO:1904047 - S-adenosyl-L-methionine binding
                     [Evidence IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="class I SAM-dependent methyltransferase"
                     /protein_id="HOQ52609.1"
                     /translation="MSAQLPAGQSQWAQIIARDPGHSQRYIDRFAMLAAQGHDLVGEA
                     RLIDAMMPRRSTILDAGCGPGRHCGYLHRAGHRVVGVDLDPALIAEARRVEPGPDYVV
                     GDLAAFDLPDGVPQQFDAILCAGNVLGFVHPLTRRPILTGFRDRLTPEGRAVVGFGAG
                     RGYDFGDFFADIEAAGLGREQTFSTWDLRPWRADANFVVAVLTR"
     gene            complement(2414..3307)
                     /locus_tag="PLF56_03245"
     CDS             complement(2414..3307)
                     /locus_tag="PLF56_03245"
                     /inference="COORDINATES: ab initio
                     prediction:GeneMarkS-2+"
                     /note="Derived by automated computational analysis using
                     gene prediction method: GeneMarkS-2+."
                     /codon_start=1
                     /transl_table=11
                     /product="hypothetical protein"
                     /protein_id="HOQ52610.1"
                     /translation="MDVQATRELIEGNPGTGYRRIARLVDQGELVRLRRGAVRAADEL
                     TPEQRLRIEIEATARVINHGTWFSHRSAALLHGLPVLIYPDTRPEVVRTFGGHGSRSA
                     RLHARAANLAPDESAVLDALPVTSLARTVLDLARSLSFPQALMVVDSALQRGLDRDIL
                     LGALAPGRGSRMAERAINAGDPQSESGGESESRALMICAGLPLPELQVPIYTADGRFV
                     ARPDFLWRAKRVVGEYDGEGKYDNQFGVSTADAVRAEKKRQAALEAENYLVIRWGKDA
                     LRQPGELARRIRRALASRPWF"
     gene            complement(3432..>4167)
                     /gene="rlmB"
                     /locus_tag="PLF56_03250"
     CDS             complement(3432..>4167)
                     /gene="rlmB"
                     /locus_tag="PLF56_03250"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_015069590.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_function: GO:0008173 - RNA methyltransferase activity
                     [Evidence IEA];
                     GO_process: GO:0009451 - RNA modification [Evidence IEA]"
                     /codon_start=2
                     /transl_table=11
                     /product="23S rRNA
                     (guanosine(2251)-2'-O)-methyltransferase RlmB"
                     /protein_id="HOQ52611.1"
                     /translation="DDWVFGRNPVLEALQAGLPVKRAYVADGVERDDRLRDILKYAAD
                     HGLSLLAATRSELDKLTNGGVHQGVALQLPPYEYTNADDLLAEALGAPDQALVIACDS
                     ITDPRNLGAIIRSAAAFGATGVIIPERRSAQVTAATWKTSAGAAARLPVARVTNLNRT
                     LKQYADAGFVVAGLAGEADLDVAGIPGVDGPVLLVVGSEGDGLARLVRDNCDVLVSIP
                     IGSEVESLNASVAASIALYEVTRCRG"
     assembly_gap    4168..4177
                     /estimated_length=10
                     /gap_type="within scaffold"
                     /linkage_evidence="paired-ends"
     gene            complement(<4178..4422)
                     /locus_tag="PLF56_03255"
     CDS             complement(<4178..4422)
                     /locus_tag="PLF56_03255"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_013865559.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="23S rRNA
                     (guanosine(2251)-2'-O)-methyltransferase RlmB"
                     /protein_id="HOQ52612.1"
                     /translation="MPGNSSRQGAVRKRGKGTGSGNTAGSGGRVRRGLEGKGPTPKAE
                     DRPYHKAYRKRGPNESNKSSSGRSGSAGKPRRDASRAG"
     gene            complement(4422..5834)
                     /gene="cysS"
                     /locus_tag="PLF56_03260"
     CDS             complement(4422..5834)
                     /gene="cysS"
                     /locus_tag="PLF56_03260"
                     /EC_number="6.1.1.16"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_014845801.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_component: GO:0005737 - cytoplasm [Evidence IEA];
                     GO_function: GO:0004817 - cysteine-tRNA ligase activity
                     [Evidence IEA];
                     GO_process: GO:0006423 - cysteinyl-tRNA aminoacylation
                     [Evidence IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="cysteine--tRNA ligase"
                     /protein_id="HOQ52613.1"
                     /translation="MSFHLYDSLQREIVEFQPLEPGRASIYVCGPTVQSAPHLGHMRS
                     RVNFDVLRRWLQFNGYQVTLVTNVTDIDDKILAKAESAGRPWWAHGFVFENAFNAAYR
                     ALGVPSATYEPRATGHISDMLELIATLIERGHAYQAGDSADVYFDVQSWAEYGALTDQ
                     RLADLEPDESADPRGKRDPRDFALWKATKSGEPATASWPSPWGRGRPGWHIECSAMAG
                     RYLGDTFDIHGGGLDLRFPHHENELAQSRAAGRGFARHWMHHAWVTMAGEKMSKSLGN
                     TADVTAAVAKHGGRAVRLWLAGPHYRSSIELSEAALAEAAAQLSRIDSFLDRSASLAA
                     ADESSSEVTLATDFTAAMNDDLGTPTALAVLFRTVKEGNVALESGEAETARRARRSVL
                     LMLQVFGLDPAAPEWSDASSSDDLTPVVDGLVGALLHQRAEARARKDWPAADAIRDTL
                     AGLGLKVDDTPNGPRWSLEA"
     gene            complement(6223..7590)
                     /locus_tag="PLF56_03265"
     CDS             complement(6223..7590)
                     /locus_tag="PLF56_03265"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_013865550.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="DUF4032 domain-containing protein"
                     /protein_id="HOQ52614.1"
                     /translation="MPRILSAKPDSRLIPLPWELPLTDWPTDYLVALPRGISRHVVRF
                     IKVVNDVYAAKEVIEAAAVHEYRMLQELARENTPAVEPLAVITDRRGMGGQPLDPVLV
                     TRHLDFSLPYRSLFSQGVRPETVNRLLDAMVVLLARLHLIGFLWLDVSLSNILFRRDA
                     GEFAAYLVDAETAELHDVLTRGQREHDLVIATTNLFGEFSDLQAGQLLDESLDPLTLV
                     ETISARYRDLWAELTGVEEFSGTELDRIESRVRRLNALGFDVAELDITTSADGSTVRI
                     QPKVVDAGHHTRRLLRLTGLDVEENQARRLLNDLDTYRFRTNQQSLDESLVAHQWLTQ
                     CFEPVVTAVPAHLRGKRDAAQIFHEVLDYRWYASQREHRDVPLLDAANGYIRDVLEGL
                     PDEAMNSRPLAEGSRQLANPFDPSQGFVDEEGAMPDDPWERDALDVPEPTGVLDMAAL
                     RRALG"
     gene            complement(7641..8186)
                     /locus_tag="PLF56_03270"
     CDS             complement(7641..8186)
                     /locus_tag="PLF56_03270"
                     /inference="COORDINATES: protein motif:HMM:NF015325.2"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_function: GO:0016491 - oxidoreductase activity
                     [Evidence IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="NAD(P)H-dependent oxidoreductase"
                     /protein_id="HOQ52615.1"
                     /translation="MNVLTLVGSGRRGSFNARLADAAVAALPAGTVETRFDLTALPFY
                     DADRDEEGTLGASVQALRSAVAAADAIVVASPAYNGALAAEVKNAIDTASRPRGNAVI
                     VGKPAAVLTAPFRAEAGARVIEQITMSLQIAGAQPVGAVNAPFLESFDAEGAVTPQVR
                     TAIEALVSELVQTAEQESVAA"
BASE COUNT         1291 a         2773 c         2811 g         1325 t
ORIGIN      
        1 cgagaatgcc tgcatcgcgc tcgctgagcg ttgccaccgg acggaccacg ggttcggaca
       61 tgtaatccat ggtagttcaa atcacaggct tgtcattcgt ctggtcggtc cagcgccccc
      121 ctaactatcg tgcgcccgca catgttcgcg ggccacggat acgtgagcgg gcactcaacg
      181 gcggggacgt ccaagcaggc cccggcgagt ccgccggtcg ccgcggggcc agtacaatga
      241 agggcgcgac gtcgcacccg gggcgccgtg cgccaccacg aaggacgaac gatgcagcca
      301 ctcagcggta ccgtgcagcg gtacgactgg ggcacccaca acgccatccc agccattctc
      361 ggccgccccg ccgacggccg tcccgtcgcc gagtactggc tgggcgcgca cagttcgtcc
      421 ccctcgctgg tcggcaacgg cacgctggtc gaccacatcg gctcgacacc tgcggtgctc
      481 ggcgcgcgca gccgggctgc gttcggtgaa caactgcctt tcttgatgaa gctgctgtcg
      541 gcccggcacg ccctgtcgat ccaggcgcat ccgtcacgcg agcaggccat cgagggcttc
      601 gcccgcgagt cgcgggcggg taccgctctg gatgcgccgg agcgtaccta caaggacagc
      661 tggcccaagc ccgagatcct gatcgcgctg gacgagtttc acagcctggc cggctttcgc
      721 gatccgcacg agaccgcgga gttgttcgcc gcactcgggg tcgctgagca actggactcg
      781 gtgatcgggc cgttgactga acgcaagggg ccggcagcga tggccgaggt gtttctcgac
      841 gtgctgagcc tggacgggtc gcgggcgcac ctggtcgatg agaccgtgtc cgcggcgatg
      901 catcaccgct atgacgacgg tacggtcggc gagttcgccc gcacggcact cgaactggac
      961 gcggtattcc cggccgatcc gggcattctg gccgcgctgc tgatgaaccg gctggtgctg
     1021 cgccctggcg aagcgctgtt cgtgccggcc ggcatgatgc acgcgcacct gcacggcacg
     1081 ggggtcgagg tgatggcaaa ctccgacaac gtgatccgtg gtgggctgac caacaagcac
     1141 atcgacgtct cagaactcgt cgccgtggtg gatttcaacc cgtggctgcc cgaaaggctg
     1201 caccctcgcc gcacccgtcc gggcgtgctg aagtatcaga ccccgtgcag cgagttcgag
     1261 gcgtggcgca tgctgatcga ccgcagtacc gggcccgtcc gtgtgcctgg acgagggtcg
     1321 gcgcgcatcc tgttcgccat cgatggcgcc tgcgtgctcg acaacggcga ggccaccctg
     1381 caactgccgc ggggccgggc ggcgttcctg tccgccgacg agctggacgt gttcgccacg
     1441 ggaacggccg aggtgtacgg ggcgtcctcc ggcattcgct gaggcgtttc tggggttgtg
     1501 tcagcacgcc gaccgccccc ctaagtatct cccgcctgcg caggtacgcg ggcggcagat
     1561 agttgggcgg gcgtttgggt cagcagcggg ctgcttgcgc cgtcctcgcg tcgggtacgg
     1621 cacaatggtg cgcggctggc ctcgtccggc cggtgcctcc gtagctcagt tggtagagcg
     1681 cccgccttgt aagcggatgg tcgcgggttc gattcctgtc ggaggctcgt gagtgcacag
     1741 cttccagccg gccagagcca gtgggcacag atcattgccc gtgacccggg tcattcccag
     1801 cgctatatcg accgattcgc gatgctggcc gcccagggcc acgacctggt cggcgaggca
     1861 cggctgatcg acgcgatgat gccacgccga tcgaccattc tggacgccgg ctgcgggccc
     1921 ggacggcact gcggttacct gcatcgcgcc gggcaccggg tggtcggggt cgacctcgac
     1981 ccggccctga tcgccgaggc gcggcgggtc gaacctgggc ctgactacgt ggtgggcgat
     2041 ctggcggctt tcgacctgcc cgatggcgta ccgcagcagt tcgacgcgat cctgtgcgcg
     2101 ggcaacgtgc tcggattcgt gcacccgctg acccggcggc cgatcctcac cggctttcgc
     2161 gaccgattga cccccgaggg acgcgctgtg gtcggcttcg gcgccggccg cggctacgac
     2221 ttcggcgact tcttcgccga tatcgaggcc gcggggctgg gccgcgagca gacgttctcg
     2281 acatgggacc tgcgcccctg gcgtgccgac gcgaactttg tggtggcggt gctgactcgc
     2341 tgagcgcccg cccaactatc cgcggcccac gtaggtgcgc aggcgcacga tggtgaggga
     2401 ggcgctcgaa aggtcagaac catggacggg acgcgagcgc tcgcctgatc cgacgggcca
     2461 gctcaccagg ctgccgaaga gcgtccttgc cccaccgaat cacgaggtag ttctcggcct
     2521 ccagggcagc ctgacgcttc ttctcggcgc ggacggcatc ggcggtgctg acaccgaact
     2581 ggttgtcgta cttgccctcg ccgtcgtatt cgccgaccac ccgcttggcc cgccacagga
     2641 agtccggtcg cgcgacgaag cgtccgtcgg cggtgtagat cgggacctgc agttcgggca
     2701 gcggaagtcc ggcgcagatc atcaacgcgc ggctctcgga ttcaccgccg ctctcgcttt
     2761 gtggatcgcc cgcgttgatg gcgcgctcgg ccattcggct gcctcgtccg ggagcgagag
     2821 cgcccaggag gatgtcccgg tcaagacccc gctgcagcgc agaatccacc accatcagcg
     2881 cttgtgggaa ggaaagagag cgcgcaagat cgagcaccgt gcgggccagc gacgtcaccg
     2941 gcagagcatc gagtaccgcc gattcgtcgg gggccaggtt cgccgcgcgt gcgtgcaggc
     3001 gagctgagcg gctgccgtgg ccaccgaaag tgcgaacgac ctcggggcgg gtgtcgggat
     3061 agatcagcac gggcaggccg tgcagcagtg ccgccgagcg gtggctgaac cacgtgccgt
     3121 gattgatcac gcgtgcggtg gcttcgattt cgatgcgtag ccgctgctca ggcgtcagtt
     3181 cgtcggccgc gcgcacggct cctcgacgca gccgaaccag ttcaccctga tcgacgagtc
     3241 gagcgatgcg tcgatagccg gtgcccggat tgccctcgat gagttcgcga gtcgcttgaa
     3301 cgtccatacc ttcagtgaac gaccggatgc gcgaacatgt tcacaatcgc ccggcccgcg
     3361 ccgaacgccc gcgcagctat cccacgccct cgaagcttcg caggcgcacg atagttaggg
     3421 gggcggtgga ttcagccgcg gcagcgagtg acctcgtaga gcgcgatgct ggccgccacc
     3481 gaggcgttca gcgactccac ctccgagccg atcggaatgg acaccagcac gtcacagttg
     3541 tcgcgcacca gccgtgccaa cccgtccccc tctgagccga ccaccagcag aaccggaccg
     3601 tccacgcccg ggatgccggc gacgtccagg tcggcctcgc cggccaggcc ggccaccacg
     3661 aagccggcgt cggcgtactg cttgagcgtg cggttgaggt tggtcacccg ggccaccggc
     3721 agccgcgcgg ccgctcctgc cgacgtcttc caggtggcgg ccgtcacctg cgcggaccgc
     3781 cgctccggaa tgatcacccc ggtcgcaccg aaggcggcgg ccgagcggat gatcgcgccc
     3841 aggttacggg ggtcggtgat cgagtcgcag gcgatcacca gggcctggtc tggagccccc
     3901 agcgcctcgg cgagcagatc gtccgcgttc gtgtactcat acggcggtaa ctgcagagcc
     3961 acgccctggt gcacgccgcc gttggtcagc ttgtcgagct cgctgcgggt ggcggccagc
     4021 agcgagagac catgatcggc ggcgtacttg agaatgtcgc gcaggcggtc atcacgctcg
     4081 acgccgtcgg ccacataggc gcgcttgacc ggcagcccgg cttgcagcgc ctccagcacc
     4141 gggttgcggc cgaacaccca gtcatcgnnn nnnnnnnccg gccctggacg cgtcgcggcg
     4201 cggcttgccg gcactcccac tgcgcccgga cgaggacttg ttgctctcgt tcggaccacg
     4261 cttgcgatac gccttgtgat aggggcgatc ctcggccttc ggtgtcggcc ccttgccttc
     4321 gagaccgcgc cgcacccggc caccggagcc cgcggtgttg ccgcttccgg tgcccttgcc
     4381 acgtttgcgg acggcgcctt gtcgggacga gtttccgggc atcaggcctc caagctccag
     4441 cgtggaccgt tcggggtgtc gtcgaccttg agccccaggc cggccagcgt gtcgcggatc
     4501 gcgtccgccg ccggccagtc cttgcgggcg cgggcctcgg cgcgttggtg cagcagggcg
     4561 cccaccagcc cgtccaccac cggggtcagg tcatcgctcg acgaggcgtc cgaccactcc
     4621 ggggcggcgg ggtcgagccc gaagacctgc agcatcagca gcaccgaccg ccgggcgcga
     4681 cgggcggtct cagcctcacc ggactcgagt gccacgttgc cctctttgac cgtgcggaag
     4741 agcacggcca atgcggtggg cgtgcccagg tcgtcattca ttgcggcagt gaagtccgtt
     4801 gccagagtca cttcggacga ggactcatcc gctgctgcca ggctggcgga acggtccaga
     4861 aagctgtcga tgcgactcaa ctgggccgcc gcctcggcca acgccgcctc gctgagttcg
     4921 atggacgagc ggtagtgcgg cccggccagc cacagccgga cggcgcgtcc gccgtgcttc
     4981 gcaaccgcgg cggtcacgtc ggcggtgttg ccgagtgact tggacatctt ttcgccggcc
     5041 atcgtcaccc aggcgtgatg catccagtgg cgagcgaagc cacggccggc ggcacgggac
     5101 tgggcgagtt cgttctcgtg gtgcggaaag cgcaggtcga ggcccccgcc gtgaatgtcg
     5161 aaggtgtcgc ccaggtacct gccggccatc gccgagcact cgatgtgcca gcccggacgt
     5221 ccccggcccc acgggctcgg ccatgaggcg gtcgcgggct caccggactt ggttgccttc
     5281 cacagagcga agtcgcgcgg atcacgcttg cctcgcgggt cggcggactc gtccggttcg
     5341 aggtcggcca gccgctgatc ggtcagagcc ccgtactcgg cccacgactg cacatcgaag
     5401 tagacgtccg cggagtcgcc ggcctgatac gcgtgtcccc gttcgatcag ggtggcgatc
     5461 aactcgagca tgtcggagat gtgcccggtc gctcgtggct cgtacgtggc ggacggcacc
     5521 ccgagcgccc ggtaggcagc gttgaacgcg ttctcgaaga cgaatccgtg ggcccaccac
     5581 ggacggcccg ccgactcggc cttggccaga atcttgtcgt cgatgtcggt gacgttcgtg
     5641 accagcgtga cttggtaacc gttgaactgc agccagcgcc gcagcacgtc gaagttgacc
     5701 cggctgcgca tgtgccccag gtgcggcgcg gactgcacgg taggcccgca cacgtagatc
     5761 gacgcccggc ccggctcaag gggctgaaac tcgacgattt cgcgctgcag gctgtcgtac
     5821 aggtgaaaac tcaccggctg agtgtatcgg ggtctgaccg tgacggtcgg cgagcagaat
     5881 ccggcggacc ctgcagatcg ggtggatttt gacaccgcag tcggtgccga aacccacccc
     5941 ttcgcggagc cgccgatggc tgcagtcgcg agtgacgccg gacggcctgc aacccgttag
     6001 gcgcgagggc acacctggcg tttgttgaga ccagccggac gccgcccggg cgctccgaga
     6061 tggggtcgct gcggcattcg ctcgatccag gcccaggggg cttagcccgg cttagcccgt
     6121 cgggaaggca gttacgttga gccggctggg cgtcgcgact gggcacagca ggttcgagcg
     6181 aggcgaaccg ggcggccgtg gtggcgcagg ccacgctcaa tctcagccca gggcacgccg
     6241 aagcgcggcc atgtcgagca caccggtcgg ttcgggcacg tccaacgcat cgcgctccca
     6301 cgggtcgtcg ggcatcgcac cttcttcgtc cacgaacccc tgcgacggat cgaacgggtt
     6361 ggccagttgg cggctgccct ctgccaacgg acgactgttc atcgcctcat cgggcagacc
     6421 ctcgagcacg tcgcgaatgt agccgttggc ggcgtccaac aacggcacgt cgcggtgctc
     6481 gcgctgggac gcgtaccagc gatagtccag cacctcgtga aatatctgcg ccgcgtcgcg
     6541 tttgccacgc agatgagcgg ggacggcggt gaccaccggc tcgaagcact gggtgagcca
     6601 ctggtgcgcg accagcgact cgtccaggct ctgctggttg gtgcggaacc gataggtgtc
     6661 taggtcgttg agcagccgcc gggcctggtt ctcttcgacg tcgaggccgg tgagtcgcag
     6721 cagtcgtcgg gtgtggtggc cagcatcgac caccttgggt tgaatgcgca ccgtcgagcc
     6781 gtcggccgag gtggtgatgt cgagctccgc cacatcgaaa cccagagcgt tcaaccggcg
     6841 cacccggctc tcgatacggt cgagctcggt gccagagaac tcttcgacgc cggtgagctc
     6901 agcccacagg tcgcggtaac gggcgctgat ggtttcgacg agcgtgagcg gatcgagcga
     6961 ctcatcgagc aactgacccg cttggaggtc gctgaactcg ccgaacaggt tggtggtggc
     7021 gattaccaga tcgtgctcgc gctggccccg ggtaagcacg tcatgcagtt cggcggtctc
     7081 ggcatcgacc aggtacgcgg cgaactcgcc ggcgtcgcgc cggaacagaa tgttcgacag
     7141 cgacacgtcg agccacagaa agccgatcag atgcagccgc gccagcagca ctaccatcgc
     7201 gtccaagagc cggttgaccg tctcgggacg caccccctgg ctgaacagcg atcggtacgg
     7261 cagcgagaaa tcgaggtgcc tggtgaccag caccgggtcg agcggctggc cccccatgcc
     7321 gcggcgatcg gtgatcaccg cgagcggctc caccgccggc gtgttctcgc gcgcgagttc
     7381 ttgcagcatg cggtattcat gcacggccgc agcctcgatc acctctttgg ccgcgtacac
     7441 gtcgttgacc accttgatga agcggacgac gtgacgcgag atgccgcggg gcagcgccac
     7501 caggtagtcg gtgggccaat cggtcagcgg caactcccag ggcaacggga tgagcctgga
     7561 atccggcttc gccgacagga tgcggggcat ggagtcattc tgccagcccc aaccgacaag
     7621 ttcagcgacc agacaggccg tcaggcggcc acggactcct gctcagccgt ttgcaccagc
     7681 tcggagacca gggcctcgat cgccgtccgc acctgcggcg tcacggctcc ctcggcgtcg
     7741 aacgactcga ggaagggtgc attcactgcg ccaactggct gggcgccggc gatctgcaac
     7801 gacatggtga tctgctcgat cacgcgcgcg ccggcctcgg cccggaaggg ggccgtcagc
     7861 accgccgccg gcttgccgac gatgacggcg ttgccgcgcg gacgggatgc cgtgtcgatg
     7921 gcgttcttca cctcggccgc cagcgcaccg ttgtaggcgg ggctggccac gacgatcgca
     7981 tcggcggccg caacggccga acgtaatgcc tggacgctcg cgcccagcgt gccctcttcg
     8041 tcacgatccg cgtcgtagaa gggcagcgcg gtgaggtcga atcgagtctc tacagtgccg
     8101 gccggtagcg ccgcgaccgc ggcatcggcg agccgggcgt tgaaggagcc gcggcgaccg
     8161 ctacccacca gagtcagaac gttcattctc atctccaaat agtcaagtta
//
LOCUS       DAOSUG010000060         8207 bp    DNA     linear   ENV 10-JUN-2024
DEFINITION  MAG TPA_asm: Micropruina sp. isolate AMR_MDS_2195
            NODE_4483_length_8207_cov_4.143646, whole genome shotgun sequence.
ACCESSION   DAOSUG010000060 DAOSUG010000000
VERSION     DAOSUG010000060.1
DBLINK      BioProject: PRJNA925477
            BioSample: SAMN32864262
            Sequence Read Archive: SRR4236673
KEYWORDS    WGS; ENV; Metagenome Assembled Genome; MAG; Third Party Data; TPA;
            TPA:assembly.
SOURCE      Micropruina sp. (wastewater metagenome)
  ORGANISM  Micropruina sp.
            Bacteria; Bacillati; Actinomycetota; Actinomycetes;
            Propionibacteriales; Nocardioidaceae; Micropruina.
REFERENCE   1  (bases 1 to 8207)
  AUTHORS   Abdulkadir,N., Saraiva,J.P., Zhang,J., Stolte,S., Gillor,O.,
            Harms,H. and Rocha,U.
  TITLE     Genome-centric analyses of 165 metagenomes show that mobile genetic
            elements are crucial for the transmission of antimicrobial
            resistance genes to pathogens in activated sludge and wastewater
  JOURNAL   Microbiol Spectr 12 (3), e0291823 (2024)
   PUBMED   38289113
REFERENCE   2  (bases 1 to 8207)
  AUTHORS   Abdulkadir,N.
  TITLE     Direct Submission
  JOURNAL   Submitted (24-MAR-2023) Environmental Microbiology, Helmholtz
            Center for Environmental Research -UFZ, Permoserstr 15, Leipzig
            04318, Germany
COMMENT     The annotation was added by the NCBI Prokaryotic Genome Annotation
            Pipeline (PGAP). Information about PGAP can be found here:
            https://www.ncbi.nlm.nih.gov/genome/annotation_prok/
            
            ##Genome-Assembly-Data-START##
            Assembly Method        :: SPAdes v. 3.11.1
            Genome Representation  :: Full
            Expected Final Version :: Yes
            Genome Coverage        :: Not Applicable
            Sequencing Technology  :: Illumina MiSeq
            ##Genome-Assembly-Data-END##
            
            ##Genome-Annotation-Data-START##
            Annotation Provider               :: NCBI
            Annotation Date                   :: 05/04/2023 04:57:10
            Annotation Pipeline               :: NCBI Prokaryotic Genome
                                                 Annotation Pipeline (PGAP)
            Annotation Method                 :: Best-placed reference protein
                                                 set; GeneMarkS-2+
            Annotation Software revision      :: 6.5
            Features Annotated                :: Gene; CDS; rRNA; tRNA; ncRNA
            Genes (total)                     :: 2,776
            CDSs (total)                      :: 2,750
            Genes (coding)                    :: 2,714
            CDSs (with protein)               :: 2,714
            Genes (RNA)                       :: 26
            tRNAs                             :: 24
            ncRNAs                            :: 2
            Pseudo Genes (total)              :: 36
            CDSs (without protein)            :: 36
            Pseudo Genes (ambiguous residues) :: 0 of 36
            Pseudo Genes (frameshifted)       :: 2 of 36
            Pseudo Genes (incomplete)         :: 32 of 36
            Pseudo Genes (internal stop)      :: 2 of 36
            ##Genome-Annotation-Data-END##
FEATURES             Location/Qualifiers
     source          1..8207
                     /organism="Micropruina sp."
                     /mol_type="genomic DNA"
                     /submitter_seqid="NODE_4483_length_8207_cov_4.143646"
                     /isolate="AMR_MDS_2195"
                     /isolation_source="Activated sludge of municipal
                     wastewater treatment plants"
                     /db_xref="taxon:2737536"
                     /environmental_sample
                     /geo_loc_name="Canada"
                     /lat_lon="49.25 N 123.1 W"
                     /collection_date="2015-06-16"
                     /metagenome_source="wastewater metagenome"
                     /note="metagenomic"
     gene            complement(<1..212)
                     /locus_tag="PLF56_03275"
     CDS             complement(<1..212)
                     /locus_tag="PLF56_03275"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_016455148.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="arginine--tRNA ligase"
                     /protein_id="HOQ52616.1"
                     /translation="MPSLPVLLTERFQAVTGVDPELRPATKPQFGHFQSNVALRLARS
                     EGKPPREIAQRLVDEDGAGAGKLGGE"
     gene            436..2169
                     /locus_tag="PLF56_03280"
     CDS             436..2169
                     /locus_tag="PLF56_03280"
                     /inference="COORDINATES: protein motif:HMM:NF012486.2"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_function: GO:0016491 - oxidoreductase activity
                     [Evidence IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="tyrosinase family protein"
                     /protein_id="HOQ52617.1"
                     /translation="MLTSQLFAGDALLQRIADGAPDRISRVQNATDPAVAKVQQALLL
                     WNPSVLPLHGADGTYGAETGAAVVQFKTIELQVPPQQLFDDVGPQTVVRLDAIAHAAE
                     RPPTPPPSVFVRQDVYQLQPPGAPVHPIIAAYAVAVRQLKRNAGAPHRTWAHHTQVHG
                     MNPDPGDGLRNQCQHFCWYFLPWHRMYLHAFEAICRSIIVESSEVDDDTKDTWALPYW
                     DYDRADTRELPPAFRDELLDGAPNPLFEPARDLGINSPVQFRGEPAPRLARLAPEQTT
                     AQGWFSSSPYSNPFDPAFAGTETGFNHASAPNAVPGPLEITPHGSVHVFVGGRFGKMS
                     DFDKAAGDPIFWLHHANLDRLWEVWRGSTGLGLDPRSSRFANQTFDFLDASGQRVHPS
                     CDSVLNLANLGYGYADTSIPASAGAGGPMDDPNRGPAEPRERRRPPRRVGAADEPVRL
                     QVDAATVEFATDAPQTRGDERFLVSVEHISADDLPNAEWGVYLQPTSGAPVLVGTLPL
                     FGLRESLNADAEHELSYLFDITDLVNRLDAEGRWQPELFRATLAPVNAVPEGAEPDPE
                     VVIGTIALLIQ"
     gene            2579..3181
                     /locus_tag="PLF56_03285"
     CDS             2579..3181
                     /locus_tag="PLF56_03285"
                     /inference="COORDINATES: protein motif:HMM:NF021465.2"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="DUF2182 domain-containing protein"
                     /protein_id="HOQ52618.1"
                     /translation="MSHGHHDEHAGHVHHPSFDSAMLIWVAMVIAMMLPTTIPHVRYV
                     GFGTRATRTQRSILLFALGYLTVWLVPGLVVAAIPGPASPIAVGLAALGAGAWELTPI
                     KRRALLRCCRTWPVRYAGAAADASAVEYGVRHALGCLVISGPAMIVLMLAGHPWWATI
                     ALAMLMTAQKLLTRPQRWSVLVALAWAAIAVAVGVSAAVG"
     assembly_gap    3220..3319
                     /estimated_length=100
                     /gap_type="within scaffold"
                     /linkage_evidence="paired-ends"
     gene            3661..4482
                     /locus_tag="PLF56_03290"
     CDS             3661..4482
                     /locus_tag="PLF56_03290"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_008359191.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="aldo/keto reductase"
                     /protein_id="HOQ52619.1"
                     /translation="MSIPTITLNDHHTIPQFGLGVFLMPPPQTKQHVLTALELGYRHI
                     DTAAVYGNETGVGEAIAESGVPRDELFITTKLWNDRQTDAPAALHESLDKLGLDHVDL
                     YLIHWPTPKYDTYVRAWLSLEQLQAEGLTRSIGVSNFHQEHLARVIAEGSVVPAVNQI
                     EVHPTLPQFDLVQFSTDLGIAVEAWSPLGRTADLDAPAVVQIAKTLGRTPAQVILRWH
                     VQRGLIVFPKASSRERLAENIDIFDFELDDAAMQTLNGLGTDNRVGPDPETFNRH"
     gene            complement(4590..5261)
                     /locus_tag="PLF56_03295"
     CDS             complement(4590..5261)
                     /locus_tag="PLF56_03295"
                     /inference="COORDINATES: protein motif:HMM:NF014724.2"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="ElyC/SanA/YdcF family protein"
                     /protein_id="HOQ52620.1"
                     /translation="MARRRGWVVGRVLLRLAVVGALLGTAVVVGPAWYVKAVSDDRML
                     SVDDVPAQPVALVLGAEVYADGQPSRYLRARLDVAYQLFTAGKVKVILVSGDGTEHYN
                     ETDGMREYLIERGIPAKKVVGDYHGYDTYDSCVRARQIFGVSKAIVVSQTFHLPRALA
                     ICRAIGIEAWGVGDETVKTNSELWAYGAMRELGANLKMVWDVLSQRQPTLGPRDRSVD
                     EALRG"
     gene            5329..6075
                     /locus_tag="PLF56_03300"
     CDS             5329..6075
                     /locus_tag="PLF56_03300"
                     /inference="COORDINATES: protein motif:HMM:NF013382.2"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_function: GO:0008168 - methyltransferase activity
                     [Evidence IEA];
                     GO_function: GO:0008757 - S-adenosylmethionine-dependent
                     methyltransferase activity [Evidence IEA];
                     GO_process: GO:0032259 - methylation [Evidence IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="class I SAM-dependent methyltransferase"
                     /protein_id="HOQ52621.1"
                     /translation="MTDEAGKAALAASFDRAAELYERARPSYPAEAIDWLLECGPREV
                     LDLGAGTGKLTRDLVCRVDTLYAVDPSPNMLAQLNLAVPKARTSVGTAEAIPLPDASV
                     DAVLVAQAWHWVDAERAIPEVRRVLRPGGTLGLIWNVRDESVGWVAELTRIIHASAAE
                     SFVTDREAIVAGLGGGVERLTIPWQRPMDERALLDLVASRSYVINASTMQRAQILAAV
                     DDLVRNHPDLGIGTRWSLPYLTEAYRVRLP"
     gene            6106..6540
                     /locus_tag="PLF56_03305"
     CDS             6106..6540
                     /locus_tag="PLF56_03305"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_015069043.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="molybdenum cofactor biosynthesis protein MoaE"
                     /protein_id="HOQ52622.1"
                     /translation="MSTLPARVAVAGIRDEPLDQAGLAAMVDDHRAGATVTFTGVVRN
                     HDAGRAVEGIEYVGHPSAGSIIAEVADEFVTRDGVHAIAVEHRVGMLRVGDLALVAAV
                     SASHRSQAFSTCSDLVDRIKQRLPIWKRQQFGDGTAEWSNCP"
     gene            complement(6595..7164)
                     /locus_tag="PLF56_03310"
     CDS             complement(6595..7164)
                     /locus_tag="PLF56_03310"
                     /inference="COORDINATES: protein motif:HMM:NF024214.2"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="NTP transferase domain-containing protein"
                     /protein_id="HOQ52623.1"
                     /translation="MQRFAIILGGGRSSRMGRPKRDLVLDGRSLFARAVDACRARTVI
                     VAVTPELPADLHDERVRRTCEDPPFGGPVAGIAAGLAALPPAEPDDEVLLLACDLATP
                     AEVVATLDAAPLGEDGVCLVEADGTPQYLAARYRLSALTRALGGQDSRDRSVKATMSA
                     LALHHVPAPGLAADLDTPHEAATAGVQLL"
     gene            7568..>8207
                     /locus_tag="PLF56_03315"
     CDS             7568..>8207
                     /locus_tag="PLF56_03315"
                     /inference="COORDINATES: ab initio
                     prediction:GeneMarkS-2+"
                     /note="Derived by automated computational analysis using
                     gene prediction method: GeneMarkS-2+."
                     /codon_start=1
                     /transl_table=11
                     /product="hypothetical protein"
                     /protein_id="HOQ52624.1"
                     /translation="MPAGQPQIPMHPYAILPVAVRLLCAQTGRRCRTLKEVRMPGPND
                     PLLGRKAKKLDPRLPVEHLHPEHVEPLRPERVNIEPAHPEPAHPEPERTEHLNAEHLR
                     RAPDRHKQVQLGGRTPLNRPIEPHLQRPGAIDLGQRARVEIDPRIPPVLLDPHIEWPH
                     RVPRLPEGQSQVPPAPEVTPDHFFEVIRSADLVSLVVSSSGLDLADGVLKAVA"
BASE COUNT         1279 a         2936 c         2620 g         1272 t
ORIGIN      
        1 tactcgccac ccagtttccc ggccccggca ccatcctcgt cgaccagtcg ctgcgcgatc
       61 tcgcgcgggg gtttgccctc cgacctcgcc agccgcagcg cgacgttgga ctgaaagtga
      121 ccgaactgcg gcttggtggc cggacggagc tcggggtcga cccccgtgac ggcctggaat
      181 cgttcggtca gcagcacggg cagagaaggc actggatcat ctttccatgc gaacccccgg
      241 cccggtcatc ccgcccaccc ccggcagccc ggactcggcc gtcatcccgg gattgcccgg
      301 ggatccgtgc aaccccgatg acgtatcgag gccacgacgc catggtgctg catcgagaag
      361 tggcagaagt acccctgtgc aacgccggtt cgtcgttgac aggatcgggg cgacccagag
      421 cgaccggagg atcctgtgct gacttcgcaa ctgttcgccg gggacgccct gctgcaacgc
      481 atcgcggacg gcgcccccga ccgcatctcg cgcgtccaga acgccaccga cccggccgtc
      541 gccaaggtgc agcaggcgct cctgctctgg aacccgtcgg tcctgccgct ccacggtgcc
      601 gacggcacct acggcgcgga aaccggcgcg gcggtcgtcc agttcaagac gatcgagttg
      661 caggtgccgc cgcagcagct tttcgacgac gtcggaccgc agaccgttgt gcgcctggac
      721 gcgatcgcgc acgccgccga gcgacctccc acgccacccc cctccgtttt cgtgcgccaa
      781 gacgtctacc aactgcagcc gcccggcgca cccgtccacc cgatcatcgc cgcctacgcg
      841 gtggcggtgc gccagctcaa gcgcaacgcc ggcgcgccgc atcgcacctg ggcgcatcac
      901 acgcaggtgc acggcatgaa ccccgacccc ggcgacgggc tgcgcaatca gtgccagcac
      961 ttctgctggt actttctgcc ctggcaccgc atgtacctgc acgcgttcga agccatctgc
     1021 cgctcgatca tcgtcgagtc ctccgaggtg gacgacgaca ccaaggacac ctgggcgctg
     1081 ccctactggg actacgaccg ggccgacacc cgcgagttgc cgccggcctt tcgcgatgag
     1141 ttgctggacg gggcgcccaa cccgctgttc gaaccggcac gcgatctggg gatcaactca
     1201 ccggtacagt tccgcggcga accggcaccg cgactcgccc ggctggcccc cgagcagacc
     1261 accgcacagg gctggttctc cagttcgccg tactcgaacc cgttcgaccc ggccttcgcc
     1321 ggcaccgaga ccggtttcaa ccacgccagc gcaccgaacg ccgttcccgg acccctcgaa
     1381 atcacgccgc acggctccgt ccacgtcttc gtcgggggcc gattcggcaa gatgagcgac
     1441 ttcgacaagg ccgccggcga cccgatcttc tggctgcacc acgcgaacct cgaccggttg
     1501 tgggaggtct ggcgcggcag caccggcctc ggcctcgacc cccgctcaag caggttcgca
     1561 aaccagacct tcgacttctt ggacgcctcc gggcagcgcg tgcacccgtc ctgcgacagc
     1621 gtgctcaacc tggccaacct cggctatggc tacgccgaca cctcgattcc cgcctcggca
     1681 ggcgcaggag gacccatgga cgatcccaac cgcggcccag ccgaaccgcg ggagcgacgt
     1741 cgtccaccgc gacgggtggg tgccgccgac gagcccgtcc ggttgcaggt ggacgccgcg
     1801 acggtcgagt tcgcgaccga cgcaccacag acccgcggcg acgaacgctt tctggtctcg
     1861 gtcgagcaca tcagcgccga cgacctgccg aacgccgagt ggggggtcta cctgcagccc
     1921 acgtcgggtg cgccggtgct ggtcggcacg ctgccgttgt tcggcctgcg cgagtcgttg
     1981 aatgccgatg cagagcacga gctgagctat ctgttcgaca tcaccgattt ggtgaaccga
     2041 ctggacgccg aaggtcgctg gcaacccgaa cttttccgcg ccaccctggc cccggtgaac
     2101 gccgtgcccg aaggtgccga gccagaccca gaggtcgtga tcggcacgat cgcgctgcta
     2161 atccaatgac tagccttgcc gccggtcggg ctcattccgc tggtcgagct ggtcgagacc
     2221 ccctcgccgg tccccccggt ccagccgact ccgcccactc cgctggtcga cctggtcgag
     2281 acctcctcgc ccgtcccgtc ggtcgagccc attccgccgg tgaggtaccc accatgagtg
     2341 gcgctggtcg acacggcggc gacgtgagtc cccggtgacc agttacttca gctccggcac
     2401 ccgagccgaa atcgtccagc cggagccgtc cggtcggtcg ggtcgtgtaa cgtccggcgg
     2461 tcagcgcgcg acttctcgga cccctcggcg gtggcttgcc gaccacccgg aatggctggt
     2521 tctgaccatc tcggccgccg cttggtgctg gctgtacgtg ctcctcgccg gcggtgcgat
     2581 gtcacacggg caccacgacg agcatgccgg ccacgttcat cacccgagct tcgacagcgc
     2641 catgctgatc tgggtggcaa tggtcatcgc gatgatgctg ccgaccacga ttccgcacgt
     2701 gcgctatgtg gggttcggta cccgggcgac tcgaacccag cgttcgatcc tgctgttcgc
     2761 gctcggctac ctgacggtct ggctggtgcc cggcctggtg gtggcggcca ttcccgggcc
     2821 ggcctcgccg atcgcggtgg gcctggccgc gctgggagcc ggcgcctggg aactgacccc
     2881 gatcaagcgg cgcgcactgc tgcgctgctg tcgtacctgg ccggtgcgct acgcgggagc
     2941 ggcggcggac gcgtccgccg ttgagtacgg cgtgcggcac gcgctcggct gcctggtgat
     3001 ctcgggtccg gcaatgatcg tgttgatgct ggccggtcac ccctggtggg cgacgatcgc
     3061 actagccatg ctgatgacgg ctcagaagct gctgacccgt ccccagcgct ggagcgtgtt
     3121 ggtcgccctc gcttgggcgg ccatcgccgt cgccgtcggc gtatccgctg ctgtgggctg
     3181 acggcactcc tgctttgtgg ccggctcggg cgagccctcn nnnnnnnnnn nnnnnnnnnn
     3241 nnnnnnnnnn nnnnnnnnnn nnnnnnnnnn nnnnnnnnnn nnnnnnnnnn nnnnnnnnnn
     3301 nnnnnnnnnn nnnnnnnnng cgcacgccgt cagtcttcca cttggctggt tgagtccctg
     3361 gtccgtggcc ggctcgggtg agccctcgcg cacgccgtca gtcttccact tggctggttg
     3421 agtccctggt ccgtggccgg cttgggggaa ccctcgcgcc cggcctcggt cctcccgcct
     3481 gaaatccctg caccagtgac cgacctctcg ccttggcgcg ccgtgcggcc tgcgatcagt
     3541 gcagggtttt caggcgcagc acggataagg gctcagccgc atgggctcgg gcgctcgcac
     3601 agcagcttca tggacacggc ctaggctcga ggcaaccagt cgtccgattc gaggagcctc
     3661 gtgagcattc ccaccatcac cctgaacgat caccacacca ttccgcagtt cggcctcggc
     3721 gtcttcttga tgccgcctcc gcagaccaag cagcacgtgc tgaccgcgct cgagctgggc
     3781 taccgccaca tcgacaccgc cgccgtctac ggcaatgaga ccggcgtggg cgaggcgatc
     3841 gccgaaagcg gggtaccgcg cgatgagctg ttcatcacga ccaaactctg gaacgaccgg
     3901 cagaccgatg caccggccgc tctgcacgaa tccctcgaca agctcggcct cgatcacgtg
     3961 gatctgtacc tgattcactg gccgacgccg aagtacgaca cctacgtgcg cgcctggctg
     4021 tcgctggagc aactgcaggc cgaagggctc acccgctcga tcggcgtctc gaactttcac
     4081 caggagcacc tggcccgagt gatcgccgag ggctccgtcg tgcccgccgt caatcagatc
     4141 gaggtgcacc ccaccctgcc gcagttcgac ctcgtccagt tcagcaccga tctcggcatc
     4201 gcggtcgaag cctggtcacc gctgggacgc accgctgatc tggacgcgcc cgccgtggtc
     4261 cagatcgcga agacccttgg acgcaccccc gcccaggtca tcctgcgctg gcatgtgcag
     4321 cgcggcctga tcgtgtttcc gaaggcctct tcgcgcgagc ggctggccga gaacatcgac
     4381 atcttcgact tcgagttgga cgatgccgcg atgcagaccc tgaacggcct cggcaccgac
     4441 aaccgcgtgg gccccgaccc cgagacgttc aaccggcact gagcgccccc ctcaccatct
     4501 cccgcctgcg aagctacgcg ggcgggagat agttggcggg ggttcggtga ggacgcgcgc
     4561 cactcagccc tggccgaacc tggcgccgct cacccgcgca acgcctcgtc cacgctccga
     4621 tcgcgagggc cgagggtggg ctggcgctgg ctcagcacgt cccagaccat cttgaggttc
     4681 gcacccagtt cgcgcatggc gccgtaggcc cacaactcgc tgttggtctt gaccgtctcg
     4741 tcgcctaccc cccaggcctc gatgccgatc gcccggcaga tcgccagcgc ccgcggcagg
     4801 tggaacgtct ggctgaccac gatggctttc gagaccccga agatctgccg ggcccgcacg
     4861 caggagtcgt aggtgtcgta gccgtgataa tcacccacga ccttctttgc cggaataccg
     4921 cgctcgataa ggtactcgcg catgccgtcg gtctcgttgt agtgctccgt accgtcaccg
     4981 gacaccagaa tcaccttcac cttgccggcg gtgaacaact ggtaggccac gtccagccgg
     5041 gcgcgcaggt agcgggaggg ttggccgtcc gcatacacct cggcacccag caccagggcg
     5101 accggctggg cgggcacgtc gtcgacgctc agcatgcgat catcgctgac ggccttgacg
     5161 taccaggcgg gccccaccac gaccgccgtg ccgagcagcg cgccgacgac cgcgagccgg
     5221 agcaggaccc ggcccaccac ccatccgcgt cgtcgcgcca tgcgccgatg gtaagcagca
     5281 ttccccagtt tcggacgaag ccgcgcgggg ccgggcgatg atggatccat gaccgatgaa
     5341 gccggcaagg cggcgctggc cgcgtccttc gaccgggccg ccgagttgta cgagcgggcc
     5401 cggccgtcgt atccggccga agcgatcgac tggctgctgg agtgcggacc gcgcgaggtg
     5461 ctcgatctgg gcgccggaac cggcaagctg acccgcgacc tcgtgtgccg cgtggacacc
     5521 ctctacgccg tcgaccccag cccgaacatg ctcgctcagc tcaacctcgc ggttcccaaa
     5581 gcccgcactt cagtcggtac cgccgaggcg atcccgctac ccgatgcgag cgtggatgcg
     5641 gtgctcgtgg cccaggcctg gcactgggtc gatgctgagc gggcgatccc cgaggtgcgg
     5701 cgggtgctgc gtccgggcgg aaccctcggc ctgatctgga acgtgcgcga tgagtcggtg
     5761 ggttgggtcg ccgagctcac ccgcatcatt catgcctctg cggcagagtc attcgtcacc
     5821 gaccgtgagg cgatcgtcgc cgggttgggg ggaggcgtcg agcggctgac gatcccctgg
     5881 caacgtccga tggacgagcg tgcgttgctc gacctggtcg cgtcacgcag ctatgtgatc
     5941 aacgcctcaa cgatgcagcg ggcgcagatt ctggcagccg tcgatgacct ggtgcgcaac
     6001 cacccggacc tcggaatcgg cacccggtgg tcgctcccct acctcaccga ggcttaccgg
     6061 gtcaggctgc cgtagtggtg cgcgcacgcc cgtagactgc tctggatgag caccctcccc
     6121 gcacgagtcg ccgtcgccgg catccgcgat gagccgctcg accaggccgg cctcgccgcc
     6181 atggtcgacg accaccgcgc cggcgcaacg gtcacgttca ccggggtcgt gcgcaatcac
     6241 gatgccggac gggcggtcga aggcatcgag tacgtcggcc accccagcgc cggttcgatc
     6301 atcgccgagg tggcggatga gttcgtcact cgggacgggg tgcatgcgat tgccgtcgag
     6361 caccgggtcg gcatgctgcg agtgggcgat ctggcgctgg tcgccgccgt gtcggcctct
     6421 caccgaagcc aggcgttcag tacttgctcc gacctggtcg accggatcaa gcagcggctg
     6481 ccgatctgga agcgtcagca gttcggcgat ggcaccgccg aatggtcgaa ctgcccgtaa
     6541 cggccggtgg tcgcgcccgc cgagaccccc ggacggcagg tgacttcgac cagttcacag
     6601 cagttgcaca cccgccgtcg cagcctcatg cggggtgtcg aggtcggcgg ccaggccggg
     6661 ggctggcacg tgatgcaggg ccagcgcgga catcgtcgcc ttgaccgagc ggtctcgcga
     6721 gtcctgcccc cccaacgccc tggtcagggc agagagccgg taacgggccg ccaggtactg
     6781 gggtgtgcca tccgcctcga ccaggcacac accgtcctca cccaacggag cggcgtcgag
     6841 cgtggccacc acctcggcgg gcgtggcgag gtcgcaggcg agcagcagca cctcgtcgtc
     6901 cggctcggcg ggtggcagag ctgcgagacc ggctgcgatg cccgccacgg gaccgccgaa
     6961 gggtgggtcc tcgcaggtgc ggcgcacgcg ttcgtcgtgt agatcggcgg gtagttcggg
     7021 agtgactgca acgatcaccg tacgtgcgcg gcaggcgtcg acggcgcgag cgaacaacga
     7081 gcgcccgtcc agcaccaggt cacgtttggg ccgacccatc cgcgaggatc gaccgccacc
     7141 aaggatgatc gcgaaacgct gcatcggctc agcctaagcc cggatccacc gatgggcccc
     7201 gtcgcgagcg gaaccagacg ggtcgcccgg gaataccgcg cgaaggcata ccgggcgtct
     7261 cgtgggcgtg gccgtcgcga gcagaaccaa acgaccggcc tggcaagccc gtccgcgcga
     7321 aggcataccg ggcgtctcgt gagcgttgcc gacgcagcca gggtgcccgg atggggccgc
     7381 gcagtaggcg cagataggtg gatccgggct gagtccgttc gtcgatcccg aacggagttg
     7441 gctgcggtat cggacgtcgc ccgcggtaac tgacgttgcc cgcctcccaa cgcgggcaac
     7501 gtcaccttgg gcgggcaacg tcgcgcttgg tgcccctacg aacctcgacc acaggaatcc
     7561 cggtcaggtg cccgccggcc agccccagat accgatgcac ccctacgcaa ttctccccgt
     7621 ggctgtgagg ctgctgtgcg cacagactgg caggcggtgc cgcactctca aggaggttcg
     7681 aatgcccgga ccgaacgatc cgctactcgg ccgcaaggcc aagaaactgg accctcgcct
     7741 gcccgtcgag cacctgcacc ccgagcacgt cgagcccctg cgccccgagc gcgtcaacat
     7801 cgaacccgca caccctgaac ccgcacaccc tgaacccgag cgaaccgagc atctgaacgc
     7861 cgagcacctt cgccgcgcac ccgaccgcca taagcaggta caactaggcg gccgaacgcc
     7921 actcaatcgg cccatcgagc cgcacctgca gcgccccggt gcgatcgact tgggtcaacg
     7981 cgcacgggtc gagatcgacc ctcgtattcc gcccgtcctg ctggacccgc acatcgagtg
     8041 gccgcatcgc gtcccccgac tgccggaggg ccaatcccag gtgccgcccg cgcccgaggt
     8101 cacccctgat cacttcttcg aagtgatcag gtcggcggat ctggtctcgc tggtcgtcag
     8161 ctcgtccggt ctcgacctgg ccgacggagt gctgaaggct gtggcgg
//
LOCUS       DAOSUG010000061         8077 bp    DNA     linear   ENV 10-JUN-2024
DEFINITION  MAG TPA_asm: Micropruina sp. isolate AMR_MDS_2195
            NODE_4567_length_8077_cov_4.539267, whole genome shotgun sequence.
ACCESSION   DAOSUG010000061 DAOSUG010000000
VERSION     DAOSUG010000061.1
DBLINK      BioProject: PRJNA925477
            BioSample: SAMN32864262
            Sequence Read Archive: SRR4236673
KEYWORDS    WGS; ENV; Metagenome Assembled Genome; MAG; Third Party Data; TPA;
            TPA:assembly.
SOURCE      Micropruina sp. (wastewater metagenome)
  ORGANISM  Micropruina sp.
            Bacteria; Bacillati; Actinomycetota; Actinomycetes;
            Propionibacteriales; Nocardioidaceae; Micropruina.
REFERENCE   1  (bases 1 to 8077)
  AUTHORS   Abdulkadir,N., Saraiva,J.P., Zhang,J., Stolte,S., Gillor,O.,
            Harms,H. and Rocha,U.
  TITLE     Genome-centric analyses of 165 metagenomes show that mobile genetic
            elements are crucial for the transmission of antimicrobial
            resistance genes to pathogens in activated sludge and wastewater
  JOURNAL   Microbiol Spectr 12 (3), e0291823 (2024)
   PUBMED   38289113
REFERENCE   2  (bases 1 to 8077)
  AUTHORS   Abdulkadir,N.
  TITLE     Direct Submission
  JOURNAL   Submitted (24-MAR-2023) Environmental Microbiology, Helmholtz
            Center for Environmental Research -UFZ, Permoserstr 15, Leipzig
            04318, Germany
COMMENT     The annotation was added by the NCBI Prokaryotic Genome Annotation
            Pipeline (PGAP). Information about PGAP can be found here:
            https://www.ncbi.nlm.nih.gov/genome/annotation_prok/
            
            ##Genome-Assembly-Data-START##
            Assembly Method        :: SPAdes v. 3.11.1
            Genome Representation  :: Full
            Expected Final Version :: Yes
            Genome Coverage        :: Not Applicable
            Sequencing Technology  :: Illumina MiSeq
            ##Genome-Assembly-Data-END##
            
            ##Genome-Annotation-Data-START##
            Annotation Provider               :: NCBI
            Annotation Date                   :: 05/04/2023 04:57:10
            Annotation Pipeline               :: NCBI Prokaryotic Genome
                                                 Annotation Pipeline (PGAP)
            Annotation Method                 :: Best-placed reference protein
                                                 set; GeneMarkS-2+
            Annotation Software revision      :: 6.5
            Features Annotated                :: Gene; CDS; rRNA; tRNA; ncRNA
            Genes (total)                     :: 2,776
            CDSs (total)                      :: 2,750
            Genes (coding)                    :: 2,714
            CDSs (with protein)               :: 2,714
            Genes (RNA)                       :: 26
            tRNAs                             :: 24
            ncRNAs                            :: 2
            Pseudo Genes (total)              :: 36
            CDSs (without protein)            :: 36
            Pseudo Genes (ambiguous residues) :: 0 of 36
            Pseudo Genes (frameshifted)       :: 2 of 36
            Pseudo Genes (incomplete)         :: 32 of 36
            Pseudo Genes (internal stop)      :: 2 of 36
            ##Genome-Annotation-Data-END##
FEATURES             Location/Qualifiers
     source          1..8077
                     /organism="Micropruina sp."
                     /mol_type="genomic DNA"
                     /submitter_seqid="NODE_4567_length_8077_cov_4.539267"
                     /isolate="AMR_MDS_2195"
                     /isolation_source="Activated sludge of municipal
                     wastewater treatment plants"
                     /db_xref="taxon:2737536"
                     /environmental_sample
                     /geo_loc_name="Canada"
                     /lat_lon="49.25 N 123.1 W"
                     /collection_date="2015-06-16"
                     /metagenome_source="wastewater metagenome"
                     /note="metagenomic"
     gene            complement(<1..1194)
                     /locus_tag="PLF56_03320"
     CDS             complement(<1..1194)
                     /locus_tag="PLF56_03320"
                     /inference="COORDINATES: protein motif:HMM:NF012738.2"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_process: GO:0055085 - transmembrane transport [Evidence
                     IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="ABC transporter permease"
                     /protein_id="HOQ52625.1"
                     /translation="MDPLDDLRTSTAPNKEAAIAGRIQALHLDQPWFSRYLLWLRHFV
                     TGDLGTAWRTQESVNRLLGGAIVTTVQLVLAATLIAIALGVLVGIVSALRQYTTFDYA
                     ITFVSFVLYSLPVFWVAVLLKQFLAIGVNDFLNNPTVNWFWISVFSVVGGLFWAGALG
                     GEFRRSLVIFGIATAVTFATSAFILGSGWLMHPSLGVVGVGVIGAAIAVAVTAVFAGM
                     SNKLALYSALTTAAVGIGSYIGIQWLFYYVPVNWAWLFGLLLVAIAIGLLIGWLFGGP
                     DRAVSMRGAAITAALMAGLTFVDRVMQGWPQYFNNPDIKGRPIATIGAETPGFQGDFW
                     LQQLDRFTHLLLPTIALVLISFASYTRYQRGSMLEVLGQDYIRTARAKGLSERVVVVR
                     HALRNA"
     gene            complement(1403..3241)
                     /locus_tag="PLF56_03325"
     CDS             complement(1403..3241)
                     /locus_tag="PLF56_03325"
                     /inference="COORDINATES: protein motif:HMM:NF012706.2"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="ABC transporter family substrate-binding
                     protein"
                     /protein_id="HOQ52626.1"
                     /translation="MGTKKWFAGAAGLIAAALALSACTPPAANSTATAPAASNVSVMW
                     NQPFYSVNQYSTSGNNLVNANVGYLITDTMTYYGEGLNLVNNKSFGTYEKLSDSPLKV
                     KVTLADTATWSDGVPVTAADLVLYWATYSGAFNTLSDDEVKKDQDTGAVTSTNKGSDV
                     YFDQTGVPAFSLVKDFPVIGDNGKSVTYTYSKPFADWEKNLMTAGLPAHVVGTRALGE
                     TDATKAADDVVKAFQNKDNATLSKISNVWNKDWNFKNKPADANLLVSCGPYVISDAVA
                     DQYLTLTKNPNYKGDHKPSIDTITIRFNEDPMAGVQALQNGEVQLISPQVTEDAYKAA
                     KALTNATTITGIEGSYEHIDLSVASGGAFDPKTYGGDAAKALKVKQAFLHVVPRQKIV
                     DTLVKPVNPDAAVRNSFNAVPGAPDYEAVSSANGMGTTYGDVDVAKAKQLLSEAGVTS
                     PTVRIMFAKQNVRRQGEFRLIKETAEEAGFKIVDSSSDYWSTLVFTAPDKYDVAFFAW
                     ISNGTGVTDVIPQYKTKKLGGSNNPYGFSNKQVDDLYDQLQVETDPAKQTEILKQVDK
                     LLVDNAFGLTIFQFPSMTSYSNKLSGIKPIAITPTIFWNFWEWKIS"
     gene            complement(3473..3907)
                     /locus_tag="PLF56_03330"
     CDS             complement(3473..3907)
                     /locus_tag="PLF56_03330"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_011183714.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="RDD family protein"
                     /protein_id="HOQ52627.1"
                     /translation="MSASAASDAEQYPGETLGLPETGRGSLASWRARVAALIIDWAAS
                     MALAVIFFGTGVLNQSGWRSWMILAVFLVQSSVLTGLAAGSFGQIIARIGVTRVDGQP
                     IGIARAIARQAMICLVLPTIVIGAERRALNDLALGTVVVNRR"
     gene            4166..5596
                     /gene="glnA"
                     /locus_tag="PLF56_03335"
     CDS             4166..5596
                     /gene="glnA"
                     /locus_tag="PLF56_03335"
                     /EC_number="6.3.1.2"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_004811889.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_function: GO:0004356 - glutamate-ammonia ligase
                     activity [Evidence IEA];
                     GO_process: GO:0006542 - glutamine biosynthetic process
                     [Evidence IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="type I glutamate--ammonia ligase"
                     /protein_id="HOQ52628.1"
                     /translation="MFQSADDLLAYVKDEGVETIDIRFCDLPGVMQHFTVPAGSFGPE
                     VFEDGLAFDGSSIRGFQKIHESDMALLPDPTSAFIDPFRRSKTLALSFFVHDPLTKEP
                     YSRDPRNIARKAEAHLQSTGIGDTAFFAPEAEFYIFDSVRFETKQNAGYYYIDSEAGA
                     WNSGAESDSDGRANRGYKVKYKGGYFPVAPVDHFTDLRDDMVRNLEASGLIVERAHHE
                     VGTAGQAEINYRFNTMLAAADEVMKFKYIIRNTAWAAGKTVTFMPKPIFGDNGSGMHV
                     HQSLWSNGNPLFYDENGYGQLSDMARHYIGGLLHHAPSLLAFTNPTVNSYHRLVPGFE
                     APTNLVYSARNRSACIRIPLTGSNPKAKRIEFRCPDPSSNPYLAFAACLLAGLDGIQN
                     KIEPLPPVDKDLYELPPEEHAKVPTVPPSLEGVLDKLEADHDYLLAGDVFTPDLIDTW
                     IDMKRADITAIRQRPHPHEFEMYYDL"
     gene            5957..6346
                     /locus_tag="PLF56_03340"
     CDS             5957..6346
                     /locus_tag="PLF56_03340"
                     /inference="COORDINATES: protein motif:HMM:NF016152.2"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="MmcQ/YjbR family DNA-binding protein"
                     /protein_id="HOQ52629.1"
                     /translation="MTHPRRYEPDDEYLARLRTICLALPEAAEKDAHGHPSFYTRKIF
                     AIFGGVVKGAHYDDRWSQSVLVLPDLLTFEATRDDDRFFVPAYYGPYGWIGLNFRAAA
                     VDWAEVADLVEQSYRRTAPVRLVKQLP"
     gene            6343..7095
                     /locus_tag="PLF56_03345"
     CDS             6343..7095
                     /locus_tag="PLF56_03345"
                     /inference="COORDINATES: protein motif:HMM:NF013775.2"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="aminoglycoside 3'-phosphotransferase"
                     /protein_id="HOQ52630.1"
                     /translation="MSSPLEWQTDLADVTVPDWVHTLARGQSVEPVWRNEDGGLTYRI
                     GAGREYVKAQRPGPDWHPEAEVARLAWVVQFVAAPRLLDSGRHGDLHWLHTAGLPGRS
                     AVDRPWRDRPADVVPQLGRALRRFHDTVPLDGCPFSWSVEHRVAHFSLDPRFVGAAPP
                     LDPVVCHGDACNPNFLLADDGSFSAYVDLGGLGVADRWADLAPALQSLGWNYGPGWPP
                     TFLAAYGIEPDPIKQRFYTALWDGVGVRLDRR"
     gene            complement(7197..>8077)
                     /locus_tag="PLF56_03350"
     CDS             complement(7197..>8077)
                     /locus_tag="PLF56_03350"
                     /inference="COORDINATES: protein motif:HMM:NF013098.2"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=3
                     /transl_table=11
                     /product="VOC family protein"
                     /protein_id="HOQ52631.1"
                     /translation="TELLPSTTRMGRVSLDVGDLAGQTAFYRDVLLLDVLSEDGPVVE
                     LGRGGQTLVTLRHRPDLPKSERRSAGLFHTAILHPDEGSLAATLASVAARTPQLYTGS
                     ADHLVSKAFYLDDPEGNGIELYWDRPRSEWTWRDGRVTMDALYLDPNEFIGSHLSDDA
                     RNHLATEPDRRGALDGLAGTVGHVHLKVGDAELAKRFYVDQLGFELTAQWHGAVFVAA
                     GGYHHHMAMNTWNSAGVGLRAPALGLGRVDITLPTDGDVAQAAARLRALGHQVRDTGA
                     LVELDDPWGNLVRLSS"
BASE COUNT         1400 a         2669 c         2618 g         1390 t
ORIGIN      
        1 tgcgttgcga agcgcgtggc gcaccacgac cacccgctcg ctcagaccct tggcacgcgc
       61 ggttcggatg tagtcctggc cgaggacctc cagcatcgaa ccacgctgat aacgcgtgta
      121 cgaggcgaat gagatcaaca ccagggcgat ggtgggcagc agcaggtgag tgaagcgatc
      181 cagctgttgc agccagaaat caccttgaaa accaggggtt tccgcaccga tggtggcgat
      241 cggacgaccc ttgatgtcgg ggttgttgaa gtactgcggc cacccctgca tgacccggtc
      301 gacgaaggta aggccggcca tcagcgccgc ggtgatggct gcgccgcgca tcgacaccgc
      361 acgatcgggg cccccaaaga gccaaccgat caacaggccg atcgcgatgg cgaccagcaa
      421 caggccgaac agccaggccc agttcactgg gacgtagtag aagagccact gaatgccgat
      481 gtaggagccg atgcccaccg cggcggtggt gagcgccgaa tagagggcca gcttgttgct
      541 catgcccgcg aatactgcgg tcacggcgac ggcgatcgcg gcgccgatca cacccacccc
      601 cacaacgccg agcgaaggat gcatcagcca tccggagccc aggatgaacg cgctcgttgc
      661 gaacgtgacg gcagtcgcaa taccgaagat gaccagactg cgacgaaact cgccgccgag
      721 tgcgccggcc cagaagaggc cgcccaccac cgagaagacg ctgatccaga accagttgac
      781 ggtggggttg ttcaagaagt cgttcacgcc gatagccaga aactgcttga gcagcacggc
      841 gacccaaaag acgggcagcg agtaaagcac gaacgagacg aaggtgatcg cgtagtcgaa
      901 cgtggtgtat tgacgaagcg cactcacgat gccgaccaaa acgcccagcg cgatggcgat
      961 gagggtggcg gcgaggacca gctgcacagt ggtgacgatc gcacccccga gcagacggtt
     1021 caccgactcc tgggtgcgcc aggcggtgcc cagatcaccg gtgacaaaat gccgcaacca
     1081 caagaggtac cgcgagaacc agggttgatc cagatggagt gcctgaatgc ggcccgcgat
     1141 cgccgcctcc ttgttggggg ccgtgctggt gcgcaagtca tccaggggat ccatcgccag
     1201 gtcgagcatc gcgtacatca ggatcgacag caccgccagg atgccgatgc ccagacccag
     1261 ccgacgggcc aggaatctga ccatgcatca ccccttctct cgagtaccac tgcgtattcc
     1321 ccgttgtggg gagccggaga cactccgact ccccacaacg ccgagggtga cagcccgctc
     1381 ggctcaggtc aagaccagac gatcagctga tcttccactc ccagaagttc cagaagatcg
     1441 tcggggtgat ggcgatcggc ttgatgccgg acagcttatt gctgtacgac gtcatgctcg
     1501 ggaactggaa gatcgtgaga ccgaacgcgt tgtcgaccag cagtttgtcg acctgcttga
     1561 ggatctcggt ctgcttggcc ggatcggtct cgacctgcaa ctggtcgtac aggtcgtcga
     1621 cctgcttatt cgagaagccg taggggttgt tggacccgcc gagcttcttg gtcttgtact
     1681 gcggaatcac atcggtcacg ccggtgccgt tcgagatcca ggcgaagaac gccacgtcgt
     1741 acttgtcggg cgccgtgaag acgagcgtcg accagtaatc gctggagctg tcgacgatct
     1801 tgaaaccggc ctcttcagcc gtctccttga tcagtcggaa ctcgccctgg cgccgaacgt
     1861 tctgcttcgc gaacatgatg cgcaccgtcg ggctggtgac gccggcctcg gacagcagct
     1921 gcttggcctt ggccacgtcg acgtcaccgt aggtggtgcc cataccgttg gcgctcgaga
     1981 cggcctcgta atcgggtgca ccgggcacgg cgttgaacga gttgcgcacc gcagcatcgg
     2041 ggttgaccgg cttcaccaga gtgtcgacga tcttctgccg gggaaccacg tgcaggaagg
     2101 cctgcttgac cttgagtgcc ttggcggcgt caccaccgta cgtcttcgga tcgaaggcac
     2161 caccggaggc gaccgacagg tcgatgtgct cgtagctgcc ttcgatgccg gtgatggtcg
     2221 tggcgttggt caaagccttg gcggccttgt aggcgtcctc ggtgacctgc ggcgaaatca
     2281 gttgcacttc gccgttctgc agagcctgca caccggccat cggatcctcg ttgaagcgga
     2341 tcgtgatggt gtcgatcgat ggcttgtgat cacccttgta gttggggttc ttggtcaggg
     2401 tgaggtactg gtcggccact gcgtccgaga tgacgtaggg gccgcaggac acgagcagat
     2461 tcgcgtcggc aggcttgttc ttgaagttcc agtccttgtt ccagacgttc gagatcttcg
     2521 acaacgtggc gttgtccttg ttctggaacg ctttcaccac atcgtcagcg gccttggtcg
     2581 cgtcggtctc gccgagcgcc cgggtgccca cgacgtgcgc cggcagtccg gccgtcatca
     2641 ggttcttctc ccagtcggcg aacggcttgg agtaggtgta ggtcaccgat ttgccattgt
     2701 caccgatgac cgggaagtcc ttgacgagag agaaggcggg cactccggtc tggtcgaagt
     2761 agacatcgct gcccttgttg gtcgaggtca ccgcccccgt gtcctggtcc ttcttgacct
     2821 cgtcatcgct caaggtgttg aacgcaccgc tgtaggtggc ccagtacagc accaggtctg
     2881 cggccgtgac gggcacaccg tccgaccagg tggcggtatc ggccagggtg accttgacct
     2941 tgagcggact gtcactgagc ttctcgtagg tgccgaagct cttgttgttg accaggttga
     3001 gaccttcgcc gtagtaggtc atcgtgtcgg tgatcagata gccgacgttg gcgttgacca
     3061 ggttgttgcc cgacgtcgag tactggttga ccgagtagaa cggctggttc cacatcacgg
     3121 agacgttcga cgcggccggc gccgtcgctg tcgagttcgc ggcaggcggg gtgcaggccg
     3181 aaagcgccag ggcggcggcg atcaggccgg cagcgccggc gaaccatttc ttggtgccca
     3241 atgttcctcc aagtctaggt cggcgggcgc actcggtcct tgtgggatgc ctgttcgtac
     3301 gctcgtccga taggtgagct tcgaccaagc taatcgctgt tcggcagggt cgcaatcttg
     3361 tggggcaagg gttgccggat cgttacggaa gagcaaccag cgctggcacc ggcgaccggc
     3421 gtccggccgc agacccacat cgacatggtg ctcgatgtgg ttctgcgttg cgtcagcggc
     3481 ggttgaccac cacggtgccc aacgccaggt cgttgagcgc ccggcgttcg gcgccgatca
     3541 cgatggtggg cagcacgagg cagatcatcg cctgtcgtgc aatggcccgc gcgatgccga
     3601 tcggctgccc gtcgacgcgg gtcacgccga tgcgggcgat gatctggccg aagctgccgg
     3661 cggccaggcc ggtgagcacc gacgactgca cgaggaacac ggccaggatc atccagctgc
     3721 gccagccgga ctggttgagc acccccgtgc cgaagaagat cacggccaac gccatggacg
     3781 cggcccagtc gatgatcagc gcggccaccc gggcgcgcca actggccagc gagccccgtc
     3841 cggtctcggg caggccgagg gtctcgccgg ggtactgctc ggcatcggac gcggcggagg
     3901 cactcatcgt ccatacccta ctggcgcggc ccggcggcac gttcagccgc ggggcgggcc
     3961 gccggctcat cacccgcggg gcttgccggt ggcttcaccc gcgggggcgt gccgccggga
     4021 acggtcctgc ccgggcccgc gaccccgatt gaggctcgga cgtggctcgg gtgcgtaaca
     4081 ccgctgaaac attgatggaa ccctccggaa actcggcctc cctagggtga acgcgttcta
     4141 ctccacttac ccactcggag gaaagatgtt ccaaagcgct gacgatctgt tggcctatgt
     4201 gaaggacgag ggcgtcgaga cgatcgacat tcgcttctgc gacctgcccg gcgtcatgca
     4261 gcacttcacc gttccggccg gctcgttcgg ccccgaggtg ttcgaggacg ggctggcgtt
     4321 cgacggctcg tccattcgtg gcttccagaa gatccacgag tcggacatgg ccctgctgcc
     4381 cgacccgacg agcgcgttca tcgacccgtt ccgccgctcc aagaccctgg cgctgagctt
     4441 cttcgtgcac gatccgctga ccaaggagcc ctacagccgc gacccgcgca acatcgcgcg
     4501 caaggccgag gcgcacctgc agtcgaccgg catcggcgac accgcgttct tcgcgcccga
     4561 ggccgagttc tacatcttcg actcggtgcg cttcgagacc aagcagaacg ccggctacta
     4621 ctacatcgac tccgaggccg gcgcctggaa cagcggcgcc gaatccgaca gcgacggccg
     4681 cgccaaccgt ggttacaagg tcaagtacaa gggcggctac ttcccggtcg caccggtcga
     4741 ccacttcacc gacctgcgcg acgacatggt gcgcaacctg gaggcgtccg gcctgatcgt
     4801 cgaacgcgcc caccacgagg tcggcaccgc gggccaggcc gagatcaact accgcttcaa
     4861 caccatgctg gccgcggccg atgaggtgat gaagttcaag tacatcattc gcaacaccgc
     4921 atgggccgcc ggcaagaccg tcaccttcat gccgaagccg atcttcggcg acaacggctc
     4981 gggcatgcac gtgcaccagt cgctgtggtc gaacggcaac ccgctgttct acgacgagaa
     5041 cggctacggc cagctctcgg acatggcgcg ccactacatc ggcggcctgc tgcatcacgc
     5101 gccgtcgctg ctcgccttca cgaacccgac ggtgaacagc tatcaccgcc tggtgcccgg
     5161 cttcgaggcc ccgaccaacc tggtgtactc ggcccgtaac cgctcggcct gtattcgcat
     5221 cccgctcacc gggtcgaacc ccaaggccaa gcgcatcgag ttccggtgcc ccgacccgtc
     5281 gtccaacccg tacctggcgt tcgccgcgtg cctgctggcc ggcctggacg gcattcagaa
     5341 caagatcgag ccgctgcccc cggtcgacaa ggacctctac gagctgcccc ccgaggagca
     5401 cgccaaggtt cccaccgttc cgccgtccct ggagggcgtg ctcgacaagc tcgaggccga
     5461 ccacgactac ctgctcgcag gcgacgtgtt cacccccgac ctgatcgaca cctggatcga
     5521 catgaagcgc gccgacatca ccgcgatccg gcagcgtccg cacccgcacg agttcgagat
     5581 gtactacgac ctctgacaac tagcgttgtc agtggtccat gacactcgcc ggctccgccg
     5641 gacacccgtg tactacgacc tctgacaact agcgcctagc aaactggcct ctgagctgcg
     5701 gaaacgcaga tcaggggcca gtctgttgtg ccttgcacga agtgcagcgt ggcctgcacg
     5761 catccgatca ggccacggtg accgcactgg tcccgagctg agcctgggcc gtgcgggcgt
     5821 gccccctttg cccttgaaca cgagcttcgc cagggcctcg gggttctctg ccgagtaagc
     5881 gtcggcgtca tccggattca tcgagcacga tcgcccacgc ggcgcggggg tggacggcgc
     5941 tgccatgatc gacgccatga cccatccgcg acgctacgaa ccggacgatg agtacctggc
     6001 ccggctgcgc acgatctgcc tggccctacc cgaggcggcc gagaaggatg cgcacggtca
     6061 tccgtcgttc tacaccagga agatcttcgc catcttcggc ggcgtggtga agggcgccca
     6121 ctacgacgac cgttggtcgc agtcggtgct cgtgctgccc gacctgctca cgttcgaagc
     6181 gacccgcgac gacgaccggt tctttgtgcc ggcctactac ggtccgtacg gctggatcgg
     6241 gctcaacttt cgcgccgccg cggtggactg ggccgaggtc gccgacctgg tcgagcagtc
     6301 gtaccgacgc acggcacccg tccggctggt caagcaactg ccgtgagctc accgctggag
     6361 tggcagacag atctcgcgga cgtcacggtg cccgactggg tgcacaccct ggcgcgcggg
     6421 cagtccgtcg agccggtctg gcgcaacgag gacggcgggc tgacctatcg gatcggtgcg
     6481 ggtcgtgagt acgtgaaggc gcagcggccc ggcccggatt ggcaccccga ggccgaggtg
     6541 gcccggctgg cctgggtggt gcagttcgtt gctgcgcccc ggttgctcga ctcgggacgg
     6601 cacggcgacc tgcactggct gcacaccgcc ggcctgcccg gacgctctgc ggtggatcgt
     6661 ccgtggcggg atcgtccagc cgatgtggtg ccgcaactgg ggcgcgcgct acgccggttt
     6721 catgacacgg tgccgctgga cggctgcccg ttcagctggt ccgtcgagca tcgggtcgcc
     6781 cacttcagct tggacccccg ttttgtgggt gcggctcccc cgctcgatcc ggtggtgtgt
     6841 cacggcgacg cctgcaaccc caactttctg ctcgccgacg acggcagctt cagcgcgtat
     6901 gtcgatctgg gcgggctggg cgtcgccgat cgctgggccg acctggcccc ggccctgcag
     6961 agcctcggct ggaactatgg ccccggctgg ccgcccacgt ttctcgccgc ctacggcatc
     7021 gagccggatc cgatcaagca gcggttctac accgccctgt gggacggggt aggtgtccgc
     7081 ctggaccgcc gctgacgggc gacttgccgg cggtccgcaa agacgatgtg agtgtgggcc
     7141 ttgtactcaa ggcccagact cacaccatca ttttggggcc gctcagacgc ggcgattcag
     7201 ctgctcagcc gcaccaggtt gccccacggg tcgtccagtt cgaccagtgc gccggtgtcg
     7261 cgcacctgat gacccaacgc tcgcagccgg gccgcggctt gggcgacgtc gccgtccgtg
     7321 ggcagagtga tgtcgacccg gccgagcccc agggcggggg cgcgaagccc cacgcctgcg
     7381 ctgttccagg tgttcatcgc catgtgatgg tggtagccgc cggccgccac gaacaccgcc
     7441 ccgtgccact gtgcggtcag ctcgaacccg agctggtcga cgtagaagcg cttggccaac
     7501 tcggcatcgc cgaccttcag gtgcacatgg ccaacggtgc ccgccagccc gtccagcgca
     7561 ccacgccgat cgggctcggt cgccaggtgg ttgcgggcat catcgctgag atggctgccg
     7621 atgaactcgt tggggtcgag gtacagcgcg tccatggtca cccggccgtc acgccacgtc
     7681 cattcgctgc ggggacgatc ccagtacagc tcgatgccgt tgccctcggg atcgtcgagg
     7741 tagaaggcct tgctgaccag gtgatcggcg gatccggtgt agagctgcgg ggtgcgcgca
     7801 gcgacggatg ccagggtcgc ggccagcgac ccctcgtccg ggtgcaggat cgctgtgtgg
     7861 aacagccccg cactacggcg ttcacttttg ggcagatccg gacggtgccg cagcgtcacc
     7921 agcgtctgcc cgccgcggcc cagctcgacc accggcccgt cctcgctcag cacgtccaac
     7981 agcagcacgt cgcggtagaa cgccgtctgc ccggccaggt cgccgacgtc gaggctgacc
     8041 cgtcccatcc gggtggtgct cggcaacagt tcagtca
//
LOCUS       DAOSUG010000062         7910 bp    DNA     linear   ENV 10-JUN-2024
DEFINITION  MAG TPA_asm: Micropruina sp. isolate AMR_MDS_2195
            NODE_4661_length_7910_cov_3.644940, whole genome shotgun sequence.
ACCESSION   DAOSUG010000062 DAOSUG010000000
VERSION     DAOSUG010000062.1
DBLINK      BioProject: PRJNA925477
            BioSample: SAMN32864262
            Sequence Read Archive: SRR4236673
KEYWORDS    WGS; ENV; Metagenome Assembled Genome; MAG; Third Party Data; TPA;
            TPA:assembly.
SOURCE      Micropruina sp. (wastewater metagenome)
  ORGANISM  Micropruina sp.
            Bacteria; Bacillati; Actinomycetota; Actinomycetes;
            Propionibacteriales; Nocardioidaceae; Micropruina.
REFERENCE   1  (bases 1 to 7910)
  AUTHORS   Abdulkadir,N., Saraiva,J.P., Zhang,J., Stolte,S., Gillor,O.,
            Harms,H. and Rocha,U.
  TITLE     Genome-centric analyses of 165 metagenomes show that mobile genetic
            elements are crucial for the transmission of antimicrobial
            resistance genes to pathogens in activated sludge and wastewater
  JOURNAL   Microbiol Spectr 12 (3), e0291823 (2024)
   PUBMED   38289113
REFERENCE   2  (bases 1 to 7910)
  AUTHORS   Abdulkadir,N.
  TITLE     Direct Submission
  JOURNAL   Submitted (24-MAR-2023) Environmental Microbiology, Helmholtz
            Center for Environmental Research -UFZ, Permoserstr 15, Leipzig
            04318, Germany
COMMENT     The annotation was added by the NCBI Prokaryotic Genome Annotation
            Pipeline (PGAP). Information about PGAP can be found here:
            https://www.ncbi.nlm.nih.gov/genome/annotation_prok/
            
            ##Genome-Assembly-Data-START##
            Assembly Method        :: SPAdes v. 3.11.1
            Genome Representation  :: Full
            Expected Final Version :: Yes
            Genome Coverage        :: Not Applicable
            Sequencing Technology  :: Illumina MiSeq
            ##Genome-Assembly-Data-END##
            
            ##Genome-Annotation-Data-START##
            Annotation Provider               :: NCBI
            Annotation Date                   :: 05/04/2023 04:57:10
            Annotation Pipeline               :: NCBI Prokaryotic Genome
                                                 Annotation Pipeline (PGAP)
            Annotation Method                 :: Best-placed reference protein
                                                 set; GeneMarkS-2+
            Annotation Software revision      :: 6.5
            Features Annotated                :: Gene; CDS; rRNA; tRNA; ncRNA
            Genes (total)                     :: 2,776
            CDSs (total)                      :: 2,750
            Genes (coding)                    :: 2,714
            CDSs (with protein)               :: 2,714
            Genes (RNA)                       :: 26
            tRNAs                             :: 24
            ncRNAs                            :: 2
            Pseudo Genes (total)              :: 36
            CDSs (without protein)            :: 36
            Pseudo Genes (ambiguous residues) :: 0 of 36
            Pseudo Genes (frameshifted)       :: 2 of 36
            Pseudo Genes (incomplete)         :: 32 of 36
            Pseudo Genes (internal stop)      :: 2 of 36
            ##Genome-Annotation-Data-END##
FEATURES             Location/Qualifiers
     source          1..7910
                     /organism="Micropruina sp."
                     /mol_type="genomic DNA"
                     /submitter_seqid="NODE_4661_length_7910_cov_3.644940"
                     /isolate="AMR_MDS_2195"
                     /isolation_source="Activated sludge of municipal
                     wastewater treatment plants"
                     /db_xref="taxon:2737536"
                     /environmental_sample
                     /geo_loc_name="Canada"
                     /lat_lon="49.25 N 123.1 W"
                     /collection_date="2015-06-16"
                     /metagenome_source="wastewater metagenome"
                     /note="metagenomic"
     gene            complement(<1..1259)
                     /locus_tag="PLF56_03355"
     CDS             complement(<1..1259)
                     /locus_tag="PLF56_03355"
                     /inference="COORDINATES: protein motif:HMM:NF012318.2"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="trypsin-like peptidase domain-containing
                     protein"
                     /protein_id="HOQ52632.1"
                     /translation="MTSSDNTPQRTPQGPDDWTWRAPTQQPTQPFATQPSPTQQQPTQ
                     PFATQPSPTQPQPADTQPLAGHALGGSFGQYPPPVGHAAPAYAPAGNVATAVQPRRRS
                     RAVMFVASTAIAALVGAGAGVGGYAFIGSQYGTTAAPSISSVPVAQPTTLDGSITAAA
                     EKISPSVVTITVRSGQSGDIGSGVVLDTEGHILTNDHVVSESEGTEGMSRSGSAASSI
                     TVTFEDGTTAPATVVGTSANNDLAVIKVTGVKSDLLKPATFATSDSVVVGQSVVAVGA
                     PLGLSETVTSGIVSATARPVRSGTTGNAVYQAVQTDAAINPGNSGGPLVNLNGQVIGI
                     NASGATLSSGSSSEQSGSIGLGFAIPADVAVRIGNELISTGKASDAFLGIKLQSDDSS
                     TDTTSQGVPLGEVTADGPAAKAGLQAG"
     gene            1449..2729
                     /locus_tag="PLF56_03360"
     CDS             1449..2729
                     /locus_tag="PLF56_03360"
                     /inference="COORDINATES: protein motif:HMM:NF024905.2"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="FUSC family protein"
                     /protein_id="HOQ52633.1"
                     /translation="MTDPDRNEPPPRPVNRLAANMSSLAEDAWNRLDFRRRLLLTGLG
                     LRQGVRRMRGSLVPVLTASAAACIAYLIAHNLLGHPTPFFAPVAAWICLGFTYNRVPR
                     KVLEIGAGATIGVAIGEVILLSLGAGAWQVAVGLLVAGLTARLLDRGDLFTIQSGVNS
                     MVVIGMGTLGSQMTGAGPSRLIDAFVGAAVALVLAILLPGDVLYRPRRYVSNMLAEFG
                     TALSMMGDGLSSGNRELLRDAHAQLSGVEGVLSDAETVWKSSAQIVALNPTMRRHRPQ
                     IDELGRQLELSTRALHTAETLLRQSRGVVAESGPMPAVGTLMHDSAATLHALSSAVRQ
                     WYNPSQARTKAKDLAADCAPEVAHPADWRHTALLSIMRSMAIDLLQLTGLSRDQARYY
                     LPGTGTEPTGDDSRVAGDEASGVWGSDELTAPLT"
     gene            complement(2755..3390)
                     /locus_tag="PLF56_03365"
     CDS             complement(2755..3390)
                     /locus_tag="PLF56_03365"
                     /inference="COORDINATES: protein motif:HMM:NF012655.2"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_function: GO:0003677 - DNA binding [Evidence IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="TetR family transcriptional regulator"
                     /protein_id="HOQ52634.1"
                     /translation="MSEQTFSRSYHSELRAEQAQRTRERIMQAAAHEFGTSGYQATTM
                     AAIARSAGVSTETVKAAGSKAELLIRSFETVFALQEGADSLADSPAAAGLLDARESTV
                     LNAVIDVIASANARSRRLWTQLVAASLSDRLVDQALQRLLASRRRDYRLLVDELDARG
                     LVRPDVDAELAAAELSFLMSPEGYQQLVDQTGWTETRYREWLTAAVRRTIG"
     gene            3454..4770
                     /locus_tag="PLF56_03370"
     CDS             3454..4770
                     /locus_tag="PLF56_03370"
                     /inference="COORDINATES: protein motif:HMM:NF012427.2"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_function: GO:0016757 - glycosyltransferase activity
                     [Evidence IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="glycosyltransferase"
                     /protein_id="HOQ52635.1"
                     /translation="MSSYVICCTPAHGHVMPLLTIARHLVEGGHRVRFITGGRYRDRV
                     QDCGVTHVALPADADVDLDRAGDIPERRGLKGPEAIRFDLRHLFLGPARGQYATVRAA
                     LAAEPADAVLTEPLFLGSALLDLLPRAERPPVVALGIFPLGAASRDTAPFGLGIVPMP
                     GAIGRLRNRLLQAFSDRMVFGAVQQEADDIARQVIGRPFRTGMFNWTSAADALVQFTV
                     PSFEYPRSDLPASVHFAGPLPLQPATMEPPRWWAELDEGRPVVHVTQGTVANADFGQL
                     ARPAIEGLANDDVLVVVSTGGRPVEDLGPLPGNVRAAGYLPYADLFDKVDVLVTNGGY
                     GTVQFALTHGVPVVAAGQTEDKIEVTARVGWSGAGINLRTNAAAPGQVAGAVRRVLHE
                     PGYRAAAQRIGQELRAADALGTLDRVLSSVQPAPLGSGIPVLSLDV"
     gene            5069..6829
                     /gene="leuA"
                     /locus_tag="PLF56_03375"
     CDS             5069..6829
                     /gene="leuA"
                     /locus_tag="PLF56_03375"
                     /EC_number="2.3.3.13"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_013864271.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_function: GO:0003852 - 2-isopropylmalate synthase
                     activity [Evidence IEA];
                     GO_process: GO:0009098 - leucine biosynthetic process
                     [Evidence IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="2-isopropylmalate synthase"
                     /protein_id="HOQ52636.1"
                     /translation="MTITVNQFGTRVQPTPVQQPSPMPFDRYTPFVPVDLPDRTWPSK
                     KITHAPRWLSTDLRDGNQALIDPMTPSRKKKMFDLLVKMGYKEIEVGFPSASQTDFDF
                     VRNLIVGGHIPDDVTISVLTQAREDLIERTAQSLIGAQRATIHLYNATAELFRRVVFG
                     IDEAQCIALATRGTELVMKYAEQNLRDVEFGYQYSPEIFTQTPTDYAVEVCNAVMDVW
                     QPSEGREIILNLPATVERSTPNTYADQIEYFGRHVRNRDHVAISLHPHNDRGTAVAAT
                     ELAVMAGADRVEGCLFGHGERTGNVCLVTLGMNLFSQGIDPKIDFSDIDEVRRTVEYC
                     TNMAVHPRHPYAGDLVYTAFSGSHQDAIKKGLDALANQASAEGKGIHEIPWEAPYLPI
                     DPHDVGRTYEAVIRVNSQSGKGGMAYIMKSEHHLELPRRLQMEFSRVVQRHTDTEGGE
                     VTPAELWRIFEAEYLSDTEPLELLSVPDISNGGRYTVTANVRVKGVEKTLVGEGNGAL
                     DAFADAVSILGYEVRVLDYTSHALSSGTDAIAVAYVETEIGQGDDTRVIWGVGTHESI
                     ATAAQKAVIGAINRAARTTD"
     gene            7052..7696
                     /locus_tag="PLF56_03380"
     CDS             7052..7696
                     /locus_tag="PLF56_03380"
                     /inference="COORDINATES: protein motif:HMM:NF024701.2"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_function: GO:0003677 - DNA binding [Evidence IEA];
                     GO_function: GO:0003700 - DNA-binding transcription factor
                     activity [Evidence IEA];
                     GO_process: GO:0006355 - regulation of DNA-templated
                     transcription [Evidence IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="TetR/AcrR family transcriptional regulator"
                     /protein_id="HOQ52637.1"
                     /translation="MARRPRGQLRQDILNTVIDLLVQAGDVAAVSIDAVVDRVGCTPP
                     ALYYYFPTKNDLLWQACEAEFAKLAEHIEGQVADVDADSTLAALITRGQSVLHWAAEH
                     PALYRILFMGTGRDGVIRGQTWDDPSLQAIVRNLDSAKSEGLVRPELDSQLVTLTLWG
                     TVHGFASLTVSFPAIPVDVVGASMNAACAPILRSMLTPVGLQSLDALLVRYPGV"
     gene            7732..>7910
                     /locus_tag="PLF56_03385"
     CDS             7732..>7910
                     /locus_tag="PLF56_03385"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_002540685.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_function: GO:0004055 - argininosuccinate synthase
                     activity [Evidence IEA];
                     GO_function: GO:0005524 - ATP binding [Evidence IEA];
                     GO_process: GO:0006526 - arginine biosynthetic process
                     [Evidence IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="argininosuccinate synthase"
                     /protein_id="HOQ52638.1"
                     /translation="MGPMPKVLTKLPIGERVGLAFSGGLDTSVAVAWMREKGSVPCTY
                     TANLGQYDEPDIDSVP"
BASE COUNT         1232 a         2700 c         2719 g         1259 t
ORIGIN      
        1 tcaccggcct gcagtcccgc cttggcggcg ggcccgtccg cggtcacctc gccgagcggg
       61 acgccctgcg acgtggtgtc ggtcgagctg tcgtccgact gcagcttgat gcccaggaac
      121 gcatcggacg ccttcccggt gctgatcagc tcgttgccga tccgcacggc gacgtcggcg
      181 gggatcgcga agcccagccc gatgctgccc gactgttccg acgaggagcc gctcgacagg
      241 gtcgcgcccg aggcgttgat gccgatcacc tggccgttga ggttgaccag cggtccgccg
      301 gagttgccgg gattgatcgc ggcgtcggtc tgcacggcct gatagaccgc gttgccggtg
      361 gtgcccgaac gaacgggacg ggcggtggcc gagacgatgc cgctggtgac cgtctcgctc
      421 aaccccagcg gggcgccgac ggcgaccacg gactggccga ccaccaccga gtcggacgtg
      481 gcgaacgtgg ccggcttgag caggtcggac ttgacgccag tcaccttgat cacggccagg
      541 tcgttgttgg ccgaggtgcc gacgacggtg gcgggggcgg tcgtgccgtc ctcgaacgtg
      601 acggtgatgc tgctggcggc gctgccgctg cgcgacatgc cctcggtgcc ttcggactcg
      661 ctgaccacgt ggtcgttggt cagaatgtgg ccctcggtgt cgagcaccac accactgccg
      721 atgtcgccgc tctggcctga tcgtacggtg atggtcacca ccgacggcga gatcttctcg
      781 gccgccgcgg tgatgctgcc gtccagcgtg gtcggctgcg cgaccggaac cgagctgatc
      841 gacggcgcgg cggtggtgcc gtactgggag ccgatgaagg cgtagccgcc gactccggcg
      901 ccggcgccga ccagggccgc gatcgcggtg gaggcgacga acatgaccgc ccgggaacgc
      961 cgtcgcggct ggacggcggt ggcgacgttg cccgccggcg catacgccgg ggcggcgtgc
     1021 ccaacgggcg gcgggtactg gccgaaggag ccgccgagcg cgtggccggc caatggctgg
     1081 gtatcagcgg gctgcggctg cgtgggtgag ggctgcgtgg cgaacggttg cgtgggctgc
     1141 tgctgcgtgg gtgagggctg cgtggcgaac ggttgcgtgg gctgctgcgt gggcgcgcgc
     1201 cacgtccagt cgtccggtcc ctgcggagtt ctctgaggtg tgttgtccga gctggtcatg
     1261 cttcgagcgt gcatggcgat cctgtgtgaa tgctgtgcgg tccccatggc ctttgtatgc
     1321 acgtcaggag gcctgcgcgg gcgacgtggt cgttgaccat gacgatggcg cgatcgccct
     1381 gcgccgaccc gcgtccgaca ccccatgacc cgaacgcgcg aaccgaggcc ggcagtaggc
     1441 tggccaaagt gaccgacccc gaccgcaacg agccgccgcc ccggcccgtc aaccggttgg
     1501 ccgccaacat gtcgtccctt gccgaggacg cctggaaccg gctcgatttt cgtcggcggc
     1561 tgctgctcac cgggctgggg ctacgacaag gcgtccggcg catgcgcggc tcgttggtgc
     1621 cggtgctcac ggccagcgcg gcggcctgca tcgcctacct gatcgcgcac aatctgctcg
     1681 gccatcccac tccgttcttc gctccggtgg cggcctggat ctgccttggc ttcacctaca
     1741 accgggtgcc gcgcaaggtg ctcgagatcg gcgccggcgc caccatcggg gtcgcgatcg
     1801 gcgaggtgat tctgctgagt cttggcgccg gggcctggca ggtggcggtc ggtctgctgg
     1861 tggcgggcct gaccgcccgc ttgctcgacc ggggtgacct gttcaccatt caaagcggtg
     1921 tgaacagcat ggtggtgatc ggcatgggca cgctcggcag ccagatgacc ggcgccggac
     1981 cgtccaggct gatcgacgcg ttcgtgggtg ccgcggtggc cctggtgctg gcgatcctgc
     2041 tgccggggga cgtgctgtac cggccgcggc ggtacgtgag caacatgctc gccgagttcg
     2101 gtaccgcgtt gagcatgatg ggcgacggac tgagcagtgg caaccgcgag ttgttgcggg
     2161 acgcccacgc ccagttgagc ggggtggaag gcgtgttgag cgacgccgaa accgtgtgga
     2221 aatcgtcggc gcagatcgtg gcgctcaacc ccacgatgcg ccggcatcgt ccgcagatcg
     2281 acgagctggg ccgccagctc gagctcagca cccgcgcgct gcacaccgcc gagacactgc
     2341 tgcggcagag ccggggcgtg gtggccgaga gcggtccgat gcccgcggtc ggcactttga
     2401 tgcacgattc ggcggccacc ctgcacgcac tgtcgagcgc cgtgcggcag tggtacaacc
     2461 cgtcgcaggc gcgcaccaag gccaaggacc tggctgccga ctgcgccccc gaggtcgcac
     2521 atccggccga ctggcggcac accgcgttgc tgtcgatcat gcgctcgatg gcgatcgacc
     2581 tgctgcagtt gaccggactg tcgcgcgatc aggcccggta ctacctgccc ggcaccggca
     2641 ccgaaccgac cggggacgat tcgcgcgtcg ccggcgacga ggcgtccggg gtgtggggct
     2701 cggacgaact gaccgcgccc ctcacctaga cgcacctaga cgtggcgggg ctgctcaacc
     2761 gatggtgcgc cgcacggctg cggtgagcca ctcccggtag cgggtctcgg tccagccggt
     2821 ctggtcgacc agttgttgat agccctcggg cgacatcagg aacgacaact cggcggccgc
     2881 cagctcggcg tcgacgtccg ggcggacgag gccgcgagcg tcgagttcgt cgaccagcag
     2941 ccgatagtcg cgccgacggc tcgccaggag gcgttgcagc gcttggtcga ccaaccgatc
     3001 ggacagggac gccgcgacga gttgcgtcca caggcgccgg ctgcgagcgt tcgccgaggc
     3061 gatcacatcg atcactgcgt tcagcacggt cgactcgcgc gcatccagca ggccggccgc
     3121 cgccggactg tccgccagcg agtcggctcc ctcctgcaac gcgaacaccg tttcgaacga
     3181 gcggatcagc aactccgcct tggacccggc cgccttgacc gtctcggtgg acacgccggc
     3241 actgcgggcg atggcggcca tcgtggtcgc ctggtagccg ctcgtgccga actcgtgcgc
     3301 ggccgcctgc atgatgcgct cgcgagtgcg ctgggcctgc tcggcgcgca gctcggagtg
     3361 ataactgcgc gagaacgtct gctcggacat attgagtttc ctcacagggt agtcaatact
     3421 ttcttgtgca acccaacact actggaggaa cccatgagtt cgtatgtcat ctgctgcact
     3481 cccgcgcacg gccacgtgat gccgttgctg accatcgccc ggcacctggt cgagggcggc
     3541 catcgggtgc ggttcatcac cggcgggcgc taccgcgacc gggtgcagga ctgcggcgtg
     3601 acccacgtcg ccctgcccgc ggacgccgac gtcgacctcg accgtgcggg tgacattcct
     3661 gagcgccggg ggttgaaggg ccccgaggcg atccggttcg acctgcggca cctgtttctg
     3721 ggcccggccc gtggccagta cgcgaccgtc cgggcagcgc ttgccgcgga gccggcggat
     3781 gccgtgttga ccgagccgtt gtttctggga tcggcgctgc tcgacctgtt gccacgcgcg
     3841 gagcgtccgc cggtggtggc cttgggaatc ttcccgctcg gcgcggcgag ccgcgacacg
     3901 gcacccttcg gactcggcat cgtgccgatg cccggcgcga tcggccgact ccgcaaccgg
     3961 ctgctgcagg cgttcagcga ccgcatggtc ttcggggccg tgcagcagga ggcggacgac
     4021 atcgcccggc aggtgatcgg gcggcccttc cgtaccggca tgttcaactg gacctcggcg
     4081 gcggacgcac tggtgcagtt cactgtgccc agcttcgagt acccccgctc ggacctgccc
     4141 gcaagcgtcc acttcgccgg accgttgcca ctgcagccgg ccaccatgga gcctccgcgt
     4201 tggtgggccg aactggacga gggccgcccg gtggtgcacg tcacccaggg cacggtcgcg
     4261 aacgccgact tcggccagct ggcgcgcccc gccatcgagg gcctggccaa cgacgacgtg
     4321 ctggtggtgg tgtcgacggg gggtcgtccg gtagaggatc tcgggccgct gccgggcaac
     4381 gtccgagccg cgggttacct gccctatgcc gacctgttcg acaaggtcga cgttctggtc
     4441 accaacggtg ggtacgggac agttcagttc gcgctcaccc acggcgtgcc ggtggtggcg
     4501 gcgggtcaga ccgaggacaa gatcgaggtg accgcccggg tgggctggtc gggcgccggg
     4561 atcaacctgc gcaccaacgc cgcagcgccg gggcaggtgg ccggcgccgt ccgtcgggtg
     4621 ctccacgagc ccgggtatcg agcggccgcg caacgaatcg ggcaggaact gcgcgcggca
     4681 gacgcgctcg gcaccctgga tcgggtactg agttcggtgc agccggcgcc tctcggcagc
     4741 ggcatacccg tgctgagcct cgacgtgtag cgcggttcgc cctgccgacc caccgccccc
     4801 ctgactatca ccagcccgcg aagcttcgcg ggctggtgat agtcaggcgg gcggtggcaa
     4861 ccaccctcgc gcggtccggg gacgccgctc gtcccattcc ggcgtggacg gcgtgacgga
     4921 tcgcgatgtt tgtgggtatg gtgacgcaca tggcattggt cagcctcatc cttggctgcc
     4981 gcagcgaggc ctcgaactag ctctcaggct tcctcgttgc ggagtgtttg cacgcgcctg
     5041 aatctccagc cctgcaacag aggatccgat gaccatcacc gtcaaccagt tcggtacgcg
     5101 cgtgcagccc accccggtgc agcagccgtc gcccatgccc ttcgaccggt acacaccctt
     5161 cgtgcccgtc gacctgcccg accgcacctg gcccagcaag aagatcaccc acgctccgcg
     5221 ctggctcagc accgacctgc gcgacggcaa ccaggcgctg atcgacccga tgacgcccag
     5281 ccgcaagaag aagatgttcg accttctggt gaagatgggt tacaaagaga tcgaggtggg
     5341 tttcccctcg gcctcgcaga ccgatttcga cttcgtccgc aacctgatcg tgggcggcca
     5401 catacccgac gacgtcacca tcagcgtcct cacccaagcc cgtgaggacc tgatcgaacg
     5461 caccgcccag tccctgatcg gggcgcagcg ggcgaccatc cacctgtaca acgcgaccgc
     5521 ggaactgttc aggcgagtgg tgttcggcat cgacgaggcg cagtgcatcg cactggccac
     5581 ccgcggtacc gagttggtga tgaagtacgc cgagcagaac ctgcgcgacg tcgagttcgg
     5641 gtaccagtac tcaccggaga tcttcaccca gactccgacc gattatgcgg tggaggtgtg
     5701 caatgcggtc atggacgtct ggcaaccgtc cgagggccgc gaaattatcc tgaacctgcc
     5761 cgccaccgtc gagcgctcca cgcccaacac ctatgccgac cagatcgagt acttcggccg
     5821 gcacgtccgc aaccgcgacc atgtcgccat ctcgctgcac ccgcacaacg accgcggcac
     5881 cgcggtggcg gccaccgagt tggcggtgat ggccggtgcc gaccgggtcg aggggtgcct
     5941 gttcggacac ggcgagcgca ccggcaacgt ctgcctggtc acccttggca tgaacctgtt
     6001 cagccagggc atcgacccga agatcgattt cagcgacatc gacgaggttc gccgcactgt
     6061 cgagtactgc accaacatgg ccgtccaccc gcgccacccg tacgcggggg atctggtcta
     6121 caccgccttc tcgggctccc accaggacgc catcaagaag ggcctcgacg cactggccaa
     6181 ccaggcctcc gctgagggca agggcattca cgagatccct tgggaagcgc cgtacctgcc
     6241 gatcgatccc cacgacgtcg gacgcaccta cgaggccgtg atccgggtga acagccagtc
     6301 cggcaagggt ggcatggcgt acatcatgaa gtccgagcac cacctggagc tgccccggcg
     6361 gctgcagatg gagttcagcc gggtcgtcca gcggcacacc gacaccgaag gtggcgaggt
     6421 caccccggcc gagctgtggc gcatcttcga ggccgaatac ctcagcgaca ccgagccgct
     6481 ggagctgctc agcgttcccg acatcagcaa cggcggccgc tacacggtca ccgccaacgt
     6541 gcgggtgaag ggcgtggaga agaccctggt cggcgagggc aacggtgcac tggacgcgtt
     6601 cgccgacgcg gtgagcattc tgggctacga ggtgcgggtg ctcgactaca cctcgcacgc
     6661 gctaagttcg ggcacggacg cgatcgcggt ggcctatgtc gaaacagaga tcggtcaggg
     6721 tgacgacacc cgggtgatct ggggcgtcgg tacccacgag tcgatcgcaa ccgcggcgca
     6781 gaaggctgtg atcggggcca tcaaccgcgc cgctcgcacc accgactgag caacacggca
     6841 ccaccgactg agcaacctcg caccatcgac tgagcaacac cgaagcacgc gaccccggcg
     6901 tcccgtccac ccttcacggt ggcgggacgt cggcgcggcc gacccaacct ccctcgatcg
     6961 gcacaggggc cgaaaacctc ctggcactgc taggtttcgg ttgggtacac gtggtggtcg
     7021 gctcggcccg gccgcaaccg aaggggacga agtggcgcgc cgtccacgcg gacaactgag
     7081 acaggacatt ctgaacaccg tcatcgacct gctggtccag gccggcgatg tcgctgcggt
     7141 atccatcgac gcggtcgtcg accgggtcgg ctgcacgccc cccgcgctct attactactt
     7201 tccgaccaag aacgacctgc tgtggcaggc gtgcgaggcc gagttcgcca agctggccga
     7261 gcacatcgaa ggccaggtcg ccgacgtcga tgccgacagc accctcgccg cactgatcac
     7321 ccgcggccag agcgtgctgc actgggccgc cgagcacccg gcgctgtacc ggattctctt
     7381 catgggcacc ggtcgcgacg gcgtgatccg tggccagacc tgggacgacc cgtcgttgca
     7441 ggccatcgtg cgcaacctgg acagcgcgaa gtccgagggg ctggtacgcc ccgaactcga
     7501 ttcccaactg gtcaccctga ccctgtgggg aaccgtgcac gggttcgcct cgctcaccgt
     7561 ctcgttcccg gcgattccgg tggacgtggt gggcgcgagc atgaacgccg cctgcgcacc
     7621 gatccttcga tcgatgttga cgccggtggg tctacagtcg ctcgacgcgt tgctggtgcg
     7681 gtaccccggg gtgtgagatc ggccggaaac cggtagccgt gggcccttag gatcggtccc
     7741 atgcccaagg ttctgacgaa acttccgatc ggcgagcggg tcggtctcgc gttctccggt
     7801 ggcctggaca cctccgtcgc cgtcgcctgg atgcgcgaaa aggggtccgt gccctgcacc
     7861 tacaccgcga acctgggcca gtacgacgag cccgacatcg actcggtgcc
//
LOCUS       DAOSUG010000063         7893 bp    DNA     linear   ENV 10-JUN-2024
DEFINITION  MAG TPA_asm: Micropruina sp. isolate AMR_MDS_2195
            NODE_4671_length_7893_cov_4.665476, whole genome shotgun sequence.
ACCESSION   DAOSUG010000063 DAOSUG010000000
VERSION     DAOSUG010000063.1
DBLINK      BioProject: PRJNA925477
            BioSample: SAMN32864262
            Sequence Read Archive: SRR4236673
KEYWORDS    WGS; ENV; Metagenome Assembled Genome; MAG; Third Party Data; TPA;
            TPA:assembly.
SOURCE      Micropruina sp. (wastewater metagenome)
  ORGANISM  Micropruina sp.
            Bacteria; Bacillati; Actinomycetota; Actinomycetes;
            Propionibacteriales; Nocardioidaceae; Micropruina.
REFERENCE   1  (bases 1 to 7893)
  AUTHORS   Abdulkadir,N., Saraiva,J.P., Zhang,J., Stolte,S., Gillor,O.,
            Harms,H. and Rocha,U.
  TITLE     Genome-centric analyses of 165 metagenomes show that mobile genetic
            elements are crucial for the transmission of antimicrobial
            resistance genes to pathogens in activated sludge and wastewater
  JOURNAL   Microbiol Spectr 12 (3), e0291823 (2024)
   PUBMED   38289113
REFERENCE   2  (bases 1 to 7893)
  AUTHORS   Abdulkadir,N.
  TITLE     Direct Submission
  JOURNAL   Submitted (24-MAR-2023) Environmental Microbiology, Helmholtz
            Center for Environmental Research -UFZ, Permoserstr 15, Leipzig
            04318, Germany
COMMENT     The annotation was added by the NCBI Prokaryotic Genome Annotation
            Pipeline (PGAP). Information about PGAP can be found here:
            https://www.ncbi.nlm.nih.gov/genome/annotation_prok/
            
            ##Genome-Assembly-Data-START##
            Assembly Method        :: SPAdes v. 3.11.1
            Genome Representation  :: Full
            Expected Final Version :: Yes
            Genome Coverage        :: Not Applicable
            Sequencing Technology  :: Illumina MiSeq
            ##Genome-Assembly-Data-END##
            
            ##Genome-Annotation-Data-START##
            Annotation Provider               :: NCBI
            Annotation Date                   :: 05/04/2023 04:57:10
            Annotation Pipeline               :: NCBI Prokaryotic Genome
                                                 Annotation Pipeline (PGAP)
            Annotation Method                 :: Best-placed reference protein
                                                 set; GeneMarkS-2+
            Annotation Software revision      :: 6.5
            Features Annotated                :: Gene; CDS; rRNA; tRNA; ncRNA
            Genes (total)                     :: 2,776
            CDSs (total)                      :: 2,750
            Genes (coding)                    :: 2,714
            CDSs (with protein)               :: 2,714
            Genes (RNA)                       :: 26
            tRNAs                             :: 24
            ncRNAs                            :: 2
            Pseudo Genes (total)              :: 36
            CDSs (without protein)            :: 36
            Pseudo Genes (ambiguous residues) :: 0 of 36
            Pseudo Genes (frameshifted)       :: 2 of 36
            Pseudo Genes (incomplete)         :: 32 of 36
            Pseudo Genes (internal stop)      :: 2 of 36
            ##Genome-Annotation-Data-END##
FEATURES             Location/Qualifiers
     source          1..7893
                     /organism="Micropruina sp."
                     /mol_type="genomic DNA"
                     /submitter_seqid="NODE_4671_length_7893_cov_4.665476"
                     /isolate="AMR_MDS_2195"
                     /isolation_source="Activated sludge of municipal
                     wastewater treatment plants"
                     /db_xref="taxon:2737536"
                     /environmental_sample
                     /geo_loc_name="Canada"
                     /lat_lon="49.25 N 123.1 W"
                     /collection_date="2015-06-16"
                     /metagenome_source="wastewater metagenome"
                     /note="metagenomic"
     gene            complement(<1..936)
                     /locus_tag="PLF56_03390"
     CDS             complement(<1..936)
                     /locus_tag="PLF56_03390"
                     /EC_number="3.1.1.103"
                     /inference="COORDINATES: protein motif:HMM:NF012371.2"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_function: GO:0016787 - hydrolase activity [Evidence
                     IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="serine hydrolase domain-containing protein"
                     /protein_id="HOQ52639.1"
                     /translation="MPNPARVSSAADPGTGTLPSDLVAKLDAAAASAYRQAATPGAIA
                     GVQTPSGTWIKAYGVADPATKAPMSVGMHTRIGSLTKPFTVTKILQLAEQGRVSLDDP
                     IATFVDGVPNGDRITLRLLANMTSGVASYSQSEAWVTTMTSAPATVFTHDELIEIGLK
                     LSPLFDPGEKFDYSNTNTLLLGKVIEKVTGQTAAAAITETVLKPLGMTHTTWPGSSPA
                     IPKPYPQGFTLQSPEASAEKPVNSTHWNPSWQWTAGELISTADDLLRGAHGIGTGQGI
                     LSAGSQAERLRSVPGATGYGLGFGCGNGWVGHSGEV"
     gene            1370..2599
                     /locus_tag="PLF56_03395"
     CDS             1370..2599
                     /locus_tag="PLF56_03395"
                     /inference="COORDINATES: ab initio
                     prediction:GeneMarkS-2+"
                     /note="Derived by automated computational analysis using
                     gene prediction method: GeneMarkS-2+."
                     /codon_start=1
                     /transl_table=11
                     /product="hypothetical protein"
                     /protein_id="HOQ52640.1"
                     /translation="MSTIARTCPMGTILITAAALSLTACSVPTPATSGAPAQPEGSPS
                     ATSTATTGGLGAAPAGNLGGAADPHPFPGQVDIGPAQPRADSDRNIYAVYDIRDGLTL
                     RDGWFAATGKWSLSGGGKGDDRAGNGGVPNVIDKRYGWVSVWGWSPGFVYSHQIYQGG
                     KPTGYWMGSKYASPETGSNSGSCDIYLGDPRQKGKWAPLSPYSCNWTNIRGWNPTPTV
                     VLRSATQVTDPDEAADILTDNCNGSDNADRCTYVGITSTTALGKLQPRGSEVINNGET
                     TAKKKISWEDEVGSETSIGVEVTNSFKLGELWKLEVKASVEHKWEYSHTFGEDIEQEV
                     DGRTVAWAEFAPNYVAFLGSWVVDAKDGKRYLLPDVSLKSPLLDGGQVQVFTCPLKDY
                     LGRYKCNVVPARVIVKP"
     gene            2671..4413
                     /locus_tag="PLF56_03400"
     CDS             2671..4413
                     /locus_tag="PLF56_03400"
                     /inference="COORDINATES: protein motif:HMM:NF019350.2"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_component: GO:0016020 - membrane [Evidence IEA];
                     GO_function: GO:0000155 - phosphorelay sensor kinase
                     activity [Evidence IEA];
                     GO_function: GO:0046983 - protein dimerization activity
                     [Evidence IEA];
                     GO_process: GO:0000160 - phosphorelay signal transduction
                     system [Evidence IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="histidine kinase"
                     /protein_id="HOQ52641.1"
                     /translation="MQPLPRSRGSRPLDLALWQLACAMAVLVVIECVLLADNTSVPYA
                     YLLGFPLIGVVYLSAGLVAWRRRPGNRMGALMVWGGLVYLLVGLANVQVPGLIALGQL
                     VASVPLALIVHLLLAFPSGRLRSRTARLVVIIGYLICTVLQLPGEMFTAQDPPFDVWF
                     VADRPELAMLASRVQLIIGVVVMVATAAILVRRLRVATGPQRRVLIPLAVYGIVAVLM
                     LPVGGSLLRSLGVAVLTTVLIQMAILAGVPIAFALSVLRGGFARTGELDDLAAWLSSG
                     APDRPRLAGAVGQALGDPTVTVGYPVDGVVVDAGGRPVVPQPGDRGQVDIDLEGRRIA
                     TIFYDAELITDAEHVAQAGRLIALQLDRERLTAELLAGREELHRSRLRVVEEADRERR
                     RIAQDLHDGIQAQLVVLALVAGRLAADEGSDASALREHAGDLRRELDRTAADLRRLVH
                     GLMPAILLERGLYAAARGFVDALPVPTELSLTGSDDGLPDVVETTAYFVLAEALTNAV
                     KYARASTLSVALERTTDRLRIVVSDDGVGGATAERGGLRGLDDRVRVLGGRLVVDSPP
                     GAGTRVWAEISCAP"
     gene            4401..5045
                     /locus_tag="PLF56_03405"
     CDS             4401..5045
                     /locus_tag="PLF56_03405"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_020578428.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_function: GO:0003677 - DNA binding [Evidence IEA];
                     GO_process: GO:0000160 - phosphorelay signal transduction
                     system [Evidence IEA];
                     GO_process: GO:0006355 - regulation of DNA-templated
                     transcription [Evidence IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="response regulator transcription factor"
                     /protein_id="HOQ52642.1"
                     /translation="MRAVIAEDHALIREGLIRILESERLSIVGVAGTPQALLDLVALE
                     TPDLVITDIRMPPTFNDEGLVAALQIRRTRPSTAVLVLSQFVHQQYALELLENDPAGV
                     GYLLKQRVGDLATFRADVERICAGGTVIDPEVVSAMLARAERGKGLGRLTPRQREVLE
                     HMAAGRSNGSIAQQLFITEKAVVQHVSGIYDTLGLPVSPDDHRRVLAVLRYLQG"
     gene            5135..6481
                     /locus_tag="PLF56_03410"
     CDS             5135..6481
                     /locus_tag="PLF56_03410"
                     /inference="COORDINATES: protein motif:HMM:NF019604.2"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_function: GO:0016491 - oxidoreductase activity
                     [Evidence IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="FAD-dependent oxidoreductase"
                     /protein_id="HOQ52643.1"
                     /translation="MPPPRFRVAIVGAGPSGLFAAQFLTARDDVAVDIFDRLPTPYGL
                     LRYGVAPDHASIKSVATALARVFEAPAVRFLGMVTFGRDVTRDELLAGYDAVIYAVGA
                     SEDLRMGVPGEDAAGSRSARAFVAWYSGHPDAEPQSLAGVRRAAAVGVGNVAVDVARM
                     LVKEPLALDATDMPADVLAEFRRAALTDVWVIGRRGPQHASFTTPELRELCSTPGVRV
                     SVQPESFDWIEDDLLDRRTRTNLTVLREASEREVPDPRVRLHFLFWRRPIELQGDPVS
                     GMVLERTVVGPDGRVAGTGETERLPVQLVLRAIGYRAVELPGVPFDHESGVIPSAQGR
                     VLRDGLVAPREYVVGWIKRGPVGVIGTNKSDAKETVQAVMADLAELGPVPERWDVVGA
                     LAARGRAPSTFEDWRRIELAEANRGASHERERMKIEAWHELLDLVQRERPGGPIPT"
     gene            6555..>7893
                     /locus_tag="PLF56_03415"
     CDS             6555..>7893
                     /locus_tag="PLF56_03415"
                     /EC_number="3.2.1.-"
                     /inference="COORDINATES: ab initio
                     prediction:GeneMarkS-2+"
                     /note="Derived by automated computational analysis using
                     gene prediction method: GeneMarkS-2+."
                     /codon_start=1
                     /transl_table=11
                     /product="sialidase family protein"
                     /protein_id="HOQ52644.1"
                     /translation="MKSHPRVLTVLVTVALLLAGCTGEPSPSPRPSDPVNAPALPATA
                     FPPLPGRGSGVETSIDAMVSDGDTLLMVATVTGRADLPVLRSSTDAGATWTDGRLTDE
                     AAAASQVHERPVGVAAVARLGSERRWLALGETDDQTFAWTSSDAQIWARTPVVGIDPD
                     NDHVEGVAGFSGGGFVAVGYHWTAARSTPRIWRSPDGITWTPSKPPGGEGSLHEVATS
                     GDQVVAVGSRDLTPMVKGRSGESLLFTSDDRGVTWASVKVPEPSDSSNFSSSLDEVTD
                     TDSGFVVGGSYHDHDEGTYRPLLLGNSDLAHWRQLRPLPEYDESSGVDELLSIGSTIV
                     VAQGSTTTDSRSKLSVHYLSPGTEGWQSASNGVSSESVTGVAAATVADGMVIGVQVDT
                     RPHTSVLWRYDTLWHRSEVPVTTLDTAPVIRPGGLLLVDGALAAYGSAQGAEVW"
BASE COUNT         1144 a         2678 c         2841 g         1230 t
ORIGIN      
        1 cacctcgccg ctgtgcccca cccagccgtt gccgcagccg aagccgagtc cgtacccggt
       61 cgcacccggc accgagcgca gccgctcggc ctgcgacccg gcgctcagaa tcccctgccc
      121 ggtgccgatg ccgtgtgcgc cgcgcagcag gtcgtccgcg gtcgagatga gttcgccggc
      181 cgtccattgc caggacgggt tccagtgcgt cgagttcacc ggcttttcgg cgctcgcctc
      241 cgggctctgc agggtgaagc cctgcgggta aggcttggga atcgcgggtg aactgcccgg
      301 ccaggtggtg tgagtcatac cgagcggctt cagcaccgtc tcggtgatcg cggcggccgc
      361 ggtctggccg gtcaccttct cgatcacctt gccgagcaga agtgtgttgg tgttggaata
      421 gtcgaacttc tcccccggat cgaacagcgg tgacagcttc agcccgatct cgatcaactc
      481 gtcgtgggtg aagaccgtcg ccggggcgct ggtcatcgtc gtcacccacg cttcgctctg
      541 gctgtagctg gccacgccgg aggtcatgtt ggccagcagc cgcagggtga tccggtcgcc
      601 gttgggcacc ccgtccacga acgtggcgat cggatcgtcc agcgacaccc tgccctgctc
      661 ggccagctgc agaatcttcg tcaccgtgaa cggcttggtc agcgacccga tccgcgtgtg
      721 catgccgacg ctcatcggtg ccttcgtggc cgggtccgca acgccgtagg ccttgatcca
      781 ggtgccggac ggggtctgta ccccggcgat cgcgcccgga gtcgcagcct gccggtaggc
      841 ggacgcggcg gcggcgtcca gcttcgccac caggtcggac ggcagcgtgc cggtgcccgg
      901 gtccgccgcg gacgagaccc gggcagggtt gggcacgcaa gccggcccgg ccgcgtcggt
      961 ggaagggctc gccgccgcac tgtttgccgc aggggcgctc ggcgctgccg ccggagcgca
     1021 tgcggtggtg gtcgccaggg cagcgacgca gagcccgagg ccgagccgtc caggcagtcg
     1081 cagccgcatc gtgcggacac ccctgccgac acctcgaatc acccagagca cggggtcgga
     1141 aatccggtga acgaatggaa gtcgtcgggg catgtcgtcc tcgtctcggt tgcctgcgcg
     1201 ggcggtaagg gatcgaacaa ccaccctgac ccacaccccc gccggcggcc aagagacctg
     1261 catcggcccc cgcacaggac tcaggtcccc tatcgactcg cgaggtggcc ccagtgcctc
     1321 cacccattgt caaaggcaga atcgagacac ccgagagagg ccgataacca tgagcaccat
     1381 cgcacgcacc tgcccgatgg ggaccatcct gatcactgcc gccgcactga gcctgacggc
     1441 ctgcagcgtg cccaccccag ccacatccgg cgccccggca cagccggagg gcagcccgtc
     1501 cgccaccagc acagcgacca ccggtggcct cggcgccgcc cccgccggca acctgggcgg
     1561 cgccgccgat ccgcacccgt tcccgggcca ggtcgacatc ggcccggcgc agccgcgggc
     1621 cgactccgac cgcaacatct acgccgtcta cgacatccgc gacggcctca ccctgcgcga
     1681 cggctggttc gccgccaccg gcaaatggag cctcagtggc ggcggcaagg gcgacgaccg
     1741 ggccggcaac ggcggcgtcc ccaacgtgat cgacaagcgc tacggctggg tgtcggtgtg
     1801 gggctggagc cccgggttcg tctattccca ccagatctac cagggcggca agcccaccgg
     1861 ctactggatg ggctccaagt acgccagccc agagaccggc tcgaacagcg gcagctgcga
     1921 catctacttg ggcgatccgc ggcagaaggg taagtgggcg ccactgagcc cctactcatg
     1981 caactggacc aacattcgcg gctggaaccc gacgcccacc gtggtgctgc gctcggccac
     2041 ccaggtgacc gacccggacg aggccgccga cattctcacc gacaactgca acggctccga
     2101 caacgccgat cgctgcacct acgtcggcat caccagcacc accgcgctgg gcaagctgca
     2161 gccacgtggc tccgaggtga tcaacaacgg cgagacgacc gcgaagaaga agatctcgtg
     2221 ggaggacgag gtcggcagcg aaacgtccat cggcgtcgag gtgaccaact cgttcaagct
     2281 cggcgagttg tggaaactcg aggtcaaggc cagcgtcgaa cacaagtggg aatacagcca
     2341 caccttcggc gaagacatcg agcaggaggt ggacggacgc accgtggcct gggccgagtt
     2401 cgcccccaac tacgtggcct tcctcggctc gtgggtggtc gacgccaagg acggcaagcg
     2461 atacctgctg cccgacgtgt cgctgaagtc gcccttgctg gacggcggtc aggtgcaggt
     2521 cttcacctgc ccgctcaagg attacctcgg acgctacaag tgcaacgtgg tgccggcccg
     2581 cgtgatcgtg aagccctgac cgtgctgagt gcgcggcctg gtggggcgcg gcctggtggg
     2641 tttcggcggg catggggcag actccgtgcc atgcagccac tcccccggtc gcgcgggtcc
     2701 cgtccgctcg atctggcgct gtggcagctc gcctgcgcga tggccgttct ggtcgtgatc
     2761 gagtgcgtgc tgctggccga caacacctcg gtgccctacg cgtacctact gggctttccg
     2821 ctgatcggcg tggtgtacct gagcgcggga ctggtggcgt ggcggcggcg tccgggcaat
     2881 cggatgggcg cgctgatggt ctggggcggc ctggtgtacc tgctggtcgg cctggccaac
     2941 gtgcaggtgc cggggctgat cgcgctgggc cagctggtgg cctccgttcc gctggcgctg
     3001 atcgtgcacc tgctgctggc ctttccgtcc ggacggctgc gcagccgcac cgccaggctc
     3061 gtggtgatca tcggatacct gatctgcacg gtgctgcagc tgccggggga gatgttcacc
     3121 gcgcaggatc cgcccttcga cgtgtggttc gtggcggatc gtcccgaact ggcgatgctg
     3181 gccagccgcg tccagttgat catcggtgtc gtggtgatgg tggccaccgc ggcgatcctg
     3241 gtgcgtcggt tgcgggtcgc caccgggccg cagcgccggg tgctgattcc gctggcggtg
     3301 tacggcatcg tggcggtgct gatgctgccg gtcggcggtt cgctgctgcg ctccctgggc
     3361 gtcgcggtgc tgaccaccgt gctgatccag atggcgatcc tggccggggt gccgatcgcg
     3421 ttcgcgctga gcgtgctgcg gggcggcttc gcccgaaccg gagagctgga cgacctcgcg
     3481 gcctggctga gcagtggcgc gccggatcgt ccgcgattgg ccggggcggt cgggcaggcg
     3541 ctgggcgacc ctacggtgac cgtcggctac ccggtcgacg gtgtggtggt ggacgccggc
     3601 ggacgtccgg tcgtgccgca gccgggcgat cgcggccagg tcgacatcga cctggagggg
     3661 cgccggatcg ccaccatctt ctacgacgcc gagctgatca ccgacgccga gcacgtcgcc
     3721 caggccggcc ggctgatcgc cctgcagctc gaccgtgagc ggctcaccgc cgaactgctg
     3781 gccggacgcg aggaactgca ccgctcccgg ctgcgggtcg tggaggaggc cgaccgtgag
     3841 cggcgccgga tcgcccagga cctgcacgac ggcattcagg cccaactggt ggtgctggcc
     3901 ttggtggccg ggcggctggc cgccgacgag ggcagcgatg cgtccgcact acgcgagcat
     3961 gccggcgacc tgcgccgcga actcgaccgg acggccgccg acctgcgccg gctggtgcac
     4021 gggctgatgc cggcgatcct gctcgagcgg gggctctacg ccgccgcccg cggattcgtg
     4081 gacgccctgc cggtgccgac cgagctgagc ctgaccggtt cggacgacgg ccttcccgac
     4141 gtggtcgaaa cgaccgccta tttcgtgctg gccgaggcct tgaccaacgc cgtcaagtac
     4201 gcgcgggcca gcacactgag cgttgcactc gagcgaacca ccgaccgatt gcgcattgtc
     4261 gtgagcgacg acggcgtcgg cggcgccacc gccgagcgcg gcgggctgcg tggactggac
     4321 gaccgcgtcc gggtgctggg tggcaggctg gtggtcgaca gcccgccggg tgcgggcacc
     4381 cgagtctggg cggagatctc atgcgcgccg tgatcgccga ggaccacgcc ctgatccgcg
     4441 agggcctgat ccgcattctg gaatccgagc ggctgagcat cgtgggggtg gccggcacgc
     4501 cgcaggcgct gctcgacctg gtggcgctgg aaacgcccga cctggtcatc accgacatcc
     4561 ggatgccgcc gacgttcaac gatgagggac tggtggcggc gctgcagatc cgccgcacgc
     4621 gtccgtccac cgcggtgctg gtgttgtcac agttcgttca ccagcagtac gccctggagt
     4681 tgctcgagaa cgatcccgcc ggcgtcggct acctgctcaa gcagcgggtc ggcgacctgg
     4741 ccacgttccg ggccgacgtg gagcggatct gcgccggcgg cacggtgatc gaccccgagg
     4801 tggtctcggc catgctggcc cgcgcggagc gtggcaaggg gttgggccgg ctgacgcctc
     4861 ggcagcgcga ggtgctcgaa cacatggccg ccggacgcag caacggatcc atcgcgcagc
     4921 aattgttcat caccgagaag gcggtggtgc agcacgtgtc cggcatctac gacaccctgg
     4981 gcctgccggt cagtcccgat gatcaccgcc gggtgctggc ggtgctgcgc tacctgcagg
     5041 gctgatcgtc gaagcgccca tgcgcgtggc ggcgaaccct gttcggccgg cggcggaggc
     5101 cccggatcgc ggctgccgag gtagggtgca cgccatgccg ccaccccgct tccgcgtcgc
     5161 catcgtgggc gccgggccgt ccgggctgtt cgcggcgcag ttcctgaccg cgcgcgacga
     5221 cgtggcagtc gacatcttcg accggctgcc cacgccgtac ggactgctgc gctatggagt
     5281 ggcgccagat catgcctcga tcaagtcggt ggcgaccgcg ctggcgcggg tgttcgaggc
     5341 gcccgccgtc cggtttctgg ggatggtgac gttcggacgt gacgtgaccc gcgacgagtt
     5401 gctggccggt tacgacgcgg tgatctatgc cgtgggcgcc agcgaggacc tgcgcatggg
     5461 ggtgcccggc gaggacgctg cgggcagccg ctcggcccgg gcgttcgtgg cctggtactc
     5521 cgggcatccg gacgccgaac cgcagtccct ggccggggtg cgccgagccg cggcggtcgg
     5581 ggtgggcaac gtggcggtgg acgtcgcccg catgctggtc aaggaaccct tggcgttgga
     5641 cgccaccgac atgcccgccg acgtgctggc cgagttccgc cgggcggcgt tgaccgacgt
     5701 ctgggtgatc ggacgccgtg ggccgcagca cgccagcttc accacgcctg agctgcgtga
     5761 gttgtgcagc acccccgggg tgcgggtgag cgtgcagccc gaatcgttcg actggatcga
     5821 ggacgacctg ctggaccggc gcacccgcac caacctgacg gtgctgcgcg aggcgtccga
     5881 acgggaggtg cccgacccgc gggtgcggct gcactttctg ttctggcgcc gaccgatcga
     5941 actacagggc gatccggtgt cgggcatggt gctggaacgc accgtggtcg gcccggacgg
     6001 acgggtggcc ggcaccggcg aaaccgagcg actgccggtg cagctggtgc tgcgcgcgat
     6061 cggctaccgg gcggtggagt tgccgggggt gccgttcgac cacgagtcgg gcgtgatccc
     6121 gtccgcgcag ggacgggtgc tgcgtgacgg cctggtggcg ccccgcgagt acgtggtggg
     6181 ttggatcaaa cgcggcccgg tcggtgtgat cggcacgaac aagtccgacg ccaaggagac
     6241 cgtgcaggcc gtgatggcgg atctggccga gctggggcca gtgcctgagc gatgggacgt
     6301 cgttggcgcc ctggcggcgc ggggacgggc gccgtccacc ttcgaggact ggcgccgtat
     6361 cgagctggcg gaggcgaacc ggggcgcatc gcacgagcgg gagcggatga agatcgaggc
     6421 gtggcacgag ctactcgatc tggtgcagcg ggagcgtccg ggcgggccga taccgacctg
     6481 agttatccac aaattctctg cgatcggccc tcagcgggcg ttctggtcgg aaccgcgggc
     6541 tagcttgctg acctatgaag agccaccccc gggtcctgac cgttctcgtc accgtggcac
     6601 tcttgctcgc cgggtgcacc ggcgagccgt cgccgtcccc ccgtcccagc gatccggtga
     6661 atgcgcccgc attgccggcg acggcctttc ctcccctgcc tggtcgcggt agcggcgtcg
     6721 agacctcgat cgacgccatg gtgagcgacg gcgacaccct gctgatggtc gcgaccgtca
     6781 ccggccgcgc agacctgccg gtgctgcgct catcgaccga tgccggcgcc acctggaccg
     6841 acggacggct caccgacgag gcggccgcgg ccagccaggt gcacgagcgt ccggtcgggg
     6901 tcgctgcggt ggcccgcctc ggtagtgagc ggcggtggtt ggccctgggc gagaccgatg
     6961 accaaacgtt cgcctggacc agttcggacg cacagatctg ggctcgcacg ccggtggtcg
     7021 gtatcgaccc cgacaacgac catgtcgaag gcgtcgccgg attctcgggc ggcggattcg
     7081 tcgcggtcgg ctaccactgg acggcggccc gcagcacgcc gcgcatctgg cgctcgcccg
     7141 acggcattac ctggaccccg tcgaaacccc ccggcggcga agggtcgctg cacgaggtgg
     7201 ccaccagcgg cgaccaggtg gtggcggtgg gttcgcgtga tctgaccccc atggtgaagg
     7261 gccgctcggg cgagtcgttg ctgttcacct cggatgatcg cggcgtcacc tgggcgtcgg
     7321 tgaaggtgcc ggaaccgtcc gactccagca acttttccag cagccttgat gaagtgaccg
     7381 acaccgattc cggctttgtg gtgggcggtt cgtaccacga ccacgacgag ggcacctatc
     7441 gcccgctgtt gctgggcaac tcggatctgg cccactggcg gcagttgcgc ccgctgccgg
     7501 agtacgacga gtcgtccggg gtcgacgagt tgctgtccat cggctcgacc atcgtggtgg
     7561 cgcagggcag caccaccacc gactcgcgta gcaagctttc ggtgcactac ctgagtccgg
     7621 gcaccgaagg atggcaatcg gcgagcaacg gcgtttcgag cgagtcggtc actggggtcg
     7681 ccgcggcgac ggtggccgat ggcatggtga tcggggtgca ggtcgatacc aggccgcaca
     7741 catccgtgct gtggcgctac gacaccctgt ggcacaggtc tgaggtaccg gtcaccacgc
     7801 tcgacaccgc accggtgatc cgtccaggcg ggttgttgct cgtcgacggt gcactcgccg
     7861 cctacggcag cgcccaggga gcggaggtct ggt
//
LOCUS       DAOSUG010000064         7875 bp    DNA     linear   ENV 10-JUN-2024
DEFINITION  MAG TPA_asm: Micropruina sp. isolate AMR_MDS_2195
            NODE_4681_length_7875_cov_4.543990, whole genome shotgun sequence.
ACCESSION   DAOSUG010000064 DAOSUG010000000
VERSION     DAOSUG010000064.1
DBLINK      BioProject: PRJNA925477
            BioSample: SAMN32864262
            Sequence Read Archive: SRR4236673
KEYWORDS    WGS; ENV; Metagenome Assembled Genome; MAG; Third Party Data; TPA;
            TPA:assembly.
SOURCE      Micropruina sp. (wastewater metagenome)
  ORGANISM  Micropruina sp.
            Bacteria; Bacillati; Actinomycetota; Actinomycetes;
            Propionibacteriales; Nocardioidaceae; Micropruina.
REFERENCE   1  (bases 1 to 7875)
  AUTHORS   Abdulkadir,N., Saraiva,J.P., Zhang,J., Stolte,S., Gillor,O.,
            Harms,H. and Rocha,U.
  TITLE     Genome-centric analyses of 165 metagenomes show that mobile genetic
            elements are crucial for the transmission of antimicrobial
            resistance genes to pathogens in activated sludge and wastewater
  JOURNAL   Microbiol Spectr 12 (3), e0291823 (2024)
   PUBMED   38289113
REFERENCE   2  (bases 1 to 7875)
  AUTHORS   Abdulkadir,N.
  TITLE     Direct Submission
  JOURNAL   Submitted (24-MAR-2023) Environmental Microbiology, Helmholtz
            Center for Environmental Research -UFZ, Permoserstr 15, Leipzig
            04318, Germany
COMMENT     The annotation was added by the NCBI Prokaryotic Genome Annotation
            Pipeline (PGAP). Information about PGAP can be found here:
            https://www.ncbi.nlm.nih.gov/genome/annotation_prok/
            
            ##Genome-Assembly-Data-START##
            Assembly Method        :: SPAdes v. 3.11.1
            Genome Representation  :: Full
            Expected Final Version :: Yes
            Genome Coverage        :: Not Applicable
            Sequencing Technology  :: Illumina MiSeq
            ##Genome-Assembly-Data-END##
            
            ##Genome-Annotation-Data-START##
            Annotation Provider               :: NCBI
            Annotation Date                   :: 05/04/2023 04:57:10
            Annotation Pipeline               :: NCBI Prokaryotic Genome
                                                 Annotation Pipeline (PGAP)
            Annotation Method                 :: Best-placed reference protein
                                                 set; GeneMarkS-2+
            Annotation Software revision      :: 6.5
            Features Annotated                :: Gene; CDS; rRNA; tRNA; ncRNA
            Genes (total)                     :: 2,776
            CDSs (total)                      :: 2,750
            Genes (coding)                    :: 2,714
            CDSs (with protein)               :: 2,714
            Genes (RNA)                       :: 26
            tRNAs                             :: 24
            ncRNAs                            :: 2
            Pseudo Genes (total)              :: 36
            CDSs (without protein)            :: 36
            Pseudo Genes (ambiguous residues) :: 0 of 36
            Pseudo Genes (frameshifted)       :: 2 of 36
            Pseudo Genes (incomplete)         :: 32 of 36
            Pseudo Genes (internal stop)      :: 2 of 36
            ##Genome-Annotation-Data-END##
FEATURES             Location/Qualifiers
     source          1..7875
                     /organism="Micropruina sp."
                     /mol_type="genomic DNA"
                     /submitter_seqid="NODE_4681_length_7875_cov_4.543990"
                     /isolate="AMR_MDS_2195"
                     /isolation_source="Activated sludge of municipal
                     wastewater treatment plants"
                     /db_xref="taxon:2737536"
                     /environmental_sample
                     /geo_loc_name="Canada"
                     /lat_lon="49.25 N 123.1 W"
                     /collection_date="2015-06-16"
                     /metagenome_source="wastewater metagenome"
                     /note="metagenomic"
     gene            complement(<1..1926)
                     /locus_tag="PLF56_03420"
     CDS             complement(<1..1926)
                     /locus_tag="PLF56_03420"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_013861913.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="biotin carboxylase N-terminal domain-containing
                     protein"
                     /protein_id="HOQ52645.1"
                     /translation="MFKRIAIVNRGEAAMRLIHAVRDLNAEHPEADPITVIALYTDAE
                     RTAMFAREADEAYPIGPAADRPYLNHDLLARVLTKARADAVWVGWGFVAEDAGFADLV
                     ESLGITFIGPSGEAMRKLGDKIGSKLIAEEVGVPVAPWSRGGLDDLDSALAAAEQVGY
                     PLMLKATAGGGGRGIRKVEGPDELTEAYQRTRDEAERAFGSGVVFLEKLVTGARHVEV
                     QVIADGQGTAWALGVRDCSVQRRNQKVIEESASPLLDRAQTDELKAAAERLALAVDYR
                     GAGTVEFLYHPQSRAFAFLEVNTRLQVEHPITEVTNDFDLVKAQIRVADGQRLTERPT
                     EFGHAVEARLNAEDPDRDFAPAPGRISRLDLPAGPGIRVDTGVAEGDSIPADFDSMIA
                     KIIAYGRDRTEALGRLRRAMGETTVVIEGGATNKSFVLDLLDQPEVVGAEGVEWADTG
                     WIDRVRAEGRLVSDRHSGVALVAAAIQAYDDAEAVEIARLLDTSYGGRPQVQHSVGRA
                     VDLKLRGATYQVTAFRIGPQRYRVRVAQGDESSEVEASVQRLDAFHSRLEVGCERFRL
                     VSATHGPVHLIEVNGVTHRVSRDEGGVLRSPAPALVVATPAAVGDQVAAGAPVLVLES
                     MKMETVLVAPFACRVKEL"
     gene            complement(2223..>2866)
                     /locus_tag="PLF56_03425"
     CDS             complement(2223..>2866)
                     /locus_tag="PLF56_03425"
                     /inference="COORDINATES: ab initio
                     prediction:GeneMarkS-2+"
                     /note="Derived by automated computational analysis using
                     gene prediction method: GeneMarkS-2+."
                     /codon_start=3
                     /transl_table=11
                     /product="hypothetical protein"
                     /protein_id="HOQ52646.1"
                     /translation="EEAERQRAEAEWYEGERRERERVAAEQAAAAEAERQRQEAEEAE
                     RLRAEADEAERAEAQRLVAEQAAVQEAERVATERAEQERAEAERLEGEAAERAEVQRL
                     ADERAEAERVDASAEPAAAEEPHAVPSEESDALEQARDSVEQASGRREVRDATEALVD
                     ELRGRYEADPDAYLRDYLEALDQLATARWQAGDWWGSRTPSKEAKALRKQHGL"
     assembly_gap    2867..2966
                     /estimated_length=100
                     /gap_type="within scaffold"
                     /linkage_evidence="paired-ends"
     gene            complement(<2967..5391)
                     /locus_tag="PLF56_03430"
     CDS             complement(<2967..5391)
                     /locus_tag="PLF56_03430"
                     /inference="COORDINATES: ab initio
                     prediction:GeneMarkS-2+"
                     /note="Derived by automated computational analysis using
                     gene prediction method: GeneMarkS-2+."
                     /codon_start=1
                     /transl_table=11
                     /product="hypothetical protein"
                     /protein_id="HOQ52647.1"
                     /translation="MNQPADTFAQRTRRLTSEVQRLRDWAGGDPTRLGAVADALVELT
                     AHRLAGHEWGEAAPSAQEAVVLSAKVLATHGPVGPYTPRADAVRSITALVHLAVIQSA
                     AGLTEGAGQTLAAALGLRGQLSRLELDADLPPAAIGWALLTWARVALAQGEPATANAR
                     ADAAASVAADLEGWLPVDIDRMVADARWAAGRPQPSVEYELRAVERYDTQARELLQQP
                     ASVPAAMLQRLSEPLFGVYSDAADRVLLIGAPGLALTLRRRLVDLLGGLAVRTDEAQP
                     LLVTALADLADDLRSVGRSAEAGDVAELAASIAGEGDQERPAPRQRTPLGAPVSWTPL
                     PPGEAFGVQTGANGEASERLAAQRGPADAEEQRLRVEVDAELARRAEQAAIEERRSAE
                     QAAQRLAQEQRRAAAEAEAARLAEAEARAVAAAEREETRRRRAERIAEHERLAAIEAA
                     QAEAELRARVNDETDQERAEREELERLAAELAVLEAAEAAELAAIERELAEAEAVDDA
                     ERVVGHAAAEAEGRIEAAEDEAVEEAEEIAAVAKRADEVAVDEAVVEAEQRADEADAA
                     ERIAADEAVAEAEQVADEAGLFVEDAESEAARQQEERQRLLAEEAELERAAVEQAELD
                     RIAAEREAAIRDAEAAAERERVEAERLAAELEAQRRAAEQAERERLEAERAEAERLAA
                     ERAAAELAAAQAERERLEAERLEAERVAAAQREDERLAAERAEAERALEAQRREAERV
                     AAERQRAEAEWYEGERRERERLAAEQAAVDEAERQRAEAEEAERLRAEAEEAERLRAE
                     AEEAERLRAE"
     gene            5666..6772
                     /locus_tag="PLF56_03435"
     CDS             5666..6772
                     /locus_tag="PLF56_03435"
                     /inference="COORDINATES: protein motif:HMM:NF016763.2"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_component: GO:0005576 - extracellular region [Evidence
                     IEA];
                     GO_process: GO:0046677 - response to antibiotic [Evidence
                     IEA];
                     GO_process: GO:0071555 - cell wall organization [Evidence
                     IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="VanZ family protein"
                     /protein_id="HOQ52648.1"
                     /translation="MLRRTHPLHQERRHMLSQFGPSTWAALLFGGVLAVLAFVPVAAY
                     RYRRAGRLRALDIVVLLIVAVYAVALWSYTLVPVPENDSFRCVGHNLRPFAFVDDVLA
                     GPRSLLANRAFLQAAFNVVLFAPFGFFLRVLRRRGFVVATLAGFGTSLAIELTQLTGV
                     WGIYHCAYRVFDVDDLLLNTTGALLGSLAGLPVRRLLSRRRPLPAVTTVTIGRRLTGM
                     AIDVLVCGLVGFTVTVGWRAIGLYVLGIPFDQLPGWIDQTLLSGVPAVLECYWVLMRG
                     RTIGEDVVQLEPVVRRSSPALARAVKYVTGVGGYLLLNSVLVPWPFAVPLFALVTIGW
                     ALRSRQHRGMSHTLAGMELRIEQAADPVGTKGTG"
     gene            6974..>7875
                     /locus_tag="PLF56_03440"
     CDS             6974..>7875
                     /locus_tag="PLF56_03440"
                     /inference="COORDINATES: protein motif:HMM:NF012311.2"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_function: GO:0008236 - serine-type peptidase activity
                     [Evidence IEA];
                     GO_process: GO:0006508 - proteolysis [Evidence IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="S8 family serine peptidase"
                     /protein_id="HOQ52649.1"
                     /translation="MLRRKLLIVAGALALTTPTLAYADTGVDRFDEAPSKAKLDTTFV
                     PASLNRDAKVTVMVEMTGDPVAVVEANTDGQLSDARAGEIRSGLKKSQDRVKSEIQDR
                     GGKVLSQMQSAFNGMRVTLPRKQVAAVSQLDGVKSVMAAPVHTIDNTVSVPFLGVPGV
                     WQHTGYLGQGVKVAIIDTGIDYTHADFGGAGTEAAFKAAKASTTFTPTVRVKGGWDFV
                     GDDYNADPSADDYQPVPHPDANPLDCQGHGSHVAGTTGGSGVNEDGTTYAGPYDTNTK
                     NKKFKVGPGVAPKVDLYALKVFGCT"
BASE COUNT         1011 a         2973 c         2580 g         1211 t
ORIGIN      
        1 cagttccttg acccggcagg cgaaaggggc gaccagaacg gtctccatct tcatcgactc
       61 cagcaccagc accggggcgc ccgcggcgac ctggtcgccg accgccgccg gcgtggcgac
      121 gaccagggcg ggggccggcg agcgcagcac gccgccctcg tcgcggctga cccggtgggt
      181 gacgccgttg acctcgatca ggtggacggg gccgtgcgtc gccgacacca gccggaaccg
      241 ctcgcaaccc acctcgagcc ggctgtgaaa ggcatccagg cgctggacgg acgcctcgac
      301 ctcgctggac tcgtcgccct gcgcgacccg gacccggtag cgctgcgggc cgatgcggaa
      361 cgcggtgacc tgataggtgg cgccgcgcag tttcaggtcg acggcccgtc caaccgaatg
      421 ctgcacctgc ggacgtccac cgtaggaggt gtcgagcagc cgggcgatct cgacggcctc
      481 ggcgtcgtcg taggcctgga tcgcggccgc caccagcgcg acgcccgaat gccggtcgct
      541 gaccaacctg ccctctgcgc gcacccggtc gatccagccc gtgtcggccc actcgacgcc
      601 ttcggcgccg accacctcgg gctggtcgag caggtcgagc acgaagctct tgttggtggc
      661 gccgccctcg atcaccaccg tggtctcgcc catggcgcgc cgcagccggc ccagggcttc
      721 ggtgcggtca cggccgtagg cgatgatctt ggcgatcatc gagtcgaagt ccgcaggaat
      781 cgaatcgccc tcggccacgc cggtgtcgac ccggatcccg ggcccggcgg gcaggtcgag
      841 ccggctgatc cgtccggggg ccggagcgaa gtcgcggtcg ggatcctcag cgttgagcct
      901 ggcctcgacg gcgtggccga actcggtcgg acgctcggtg agccgctgcc cgtccgccac
      961 ccggatctgg gccttgacca ggtcgaaatc gttggtcacc tcggtgatcg gatgctcgac
     1021 ctgcagccgg gtgttcacct cgagaaaggc gaaggcgcgg ctctgcgggt ggtagaggaa
     1081 ctcgacggtg cctgcgccgc ggtagtcgac ggccagggcc agccgctcgg cggccgcctt
     1141 gagttcgtcg gtctgggccc ggtcgagcag gggggaggcc gactcttcga tcaccttctg
     1201 gttgcggcgt tgcaccgagc agtcgcggac gccgagtgcc caggccgtgc cctggccgtc
     1261 cgcgatcacc tgcacctcga cgtgccgggc gccggtgacc agcttctcga gaaagaccac
     1321 gcccgagccg aaggcgcgtt cggcctcgtc gcgggtgcgc tgataggcct cggtgagttc
     1381 gtccgggccc tcgaccttgc ggatgccccg gccgccgccg ccggcggtgg ccttgagcat
     1441 cagcgggtag ccgacctgtt cggcggcggc gagggcgctg tcgaggtcgt ccaagcctcc
     1501 gcgggaccag ggtgcaaccg gcacccccac ctcttcggcg atcagcttgc tgccgatctt
     1561 gtcacccagc ttgcgcatgg cctcgcccga ggggccgatg aaggtgatgc cgaggctctc
     1621 gaccaggtcg gcgaaaccgg cgtcctcggc cacgaagccc caacccaccc agaccgcgtc
     1681 ggcccgggcc tttgtgagca cgcgggccag caggtcgtgg ttgaggtagg gacgatccgc
     1741 ggccggcccg atcgggtacg cctcgtctgc ctcacgggcg aacatggcgg tgcgctcggc
     1801 gtcggtgtag agcgcgatca ccgtgatcgg gtcggcctcg gggtgctcgg cgttgaggtc
     1861 gcggacggcg tggatcagcc gcatggccgc ctcgccgcga ttgacgatcg cgatgcgctt
     1921 gaacacccgg gtgcctcctc acgtgtgcag cgctcgcccg gcggagggtg cccggcgtcg
     1981 gtcggcagca ctgcctacga aatcttccac gtctgctggg ctatggcgag acgacccgag
     2041 cggcaagcgc cagattggac ggactgacgc ggtggggctg tccggaagcg ccaagtgggc
     2101 ctggacaggt ttggccggtt ctcacaacgg ctgcggcagc accgagcagc caacctgccc
     2161 atgcccgtcc agggttgcga accagcgcaa tcgcatccgg tcgagcgcct cctggtggtg
     2221 tgtcagagtc cgtgctgctt gcgcagtgcc ttcgcctcct tggacggggt gcgcgacccc
     2281 caccagtcgc ccgcctgcca gcgcgcggtg gcgagctgat cgagagcctc caggtagtcc
     2341 cgcaggtagg cgtcgggatc ggcctcgtaa cggcccctga gttcgtctac cagcgcctcg
     2401 gtggcgtcac gcacctcacg acgtccggac gcctgttcga cactatctcg ggcctgctcg
     2461 agtgcgtccg attcctcgct tgggacggca tgcggctcct cggcggccgc gggctccgca
     2521 ctcgcgtcca cccgctccgc ctcggcccgc tcgtccgcca agcgctgcac ctcggcacgc
     2581 tcggcggcct caccctccag gcgttcggcc tcagcgcgct cctgttcggc acgctcagtc
     2641 gccacccgct ccgcctcctg gaccgcggcc tgttccgcca ccaaccgttg cgcttcggca
     2701 cgttccgctt cgtcggcctc agcacgcagg cgctcggctt cctccgcctc ctgacgctgc
     2761 cgctccgcct ctgcggcggc agcctgctcc gcggccacac gttcccgctc acgtcgctcg
     2821 ccctcgtacc actcagcctc ggcacgctga cgctcggcct cctcagnnnn nnnnnnnnnn
     2881 nnnnnnnnnn nnnnnnnnnn nnnnnnnnnn nnnnnnnnnn nnnnnnnnnn nnnnnnnnnn
     2941 nnnnnnnnnn nnnnnnnnnn nnnnnncctc cgcacgcaga cgctccgcct cctcagcctc
     3001 cgcacgcaga cgctccgcct cctcagcctc cgcacgcaga cgctccgcct cttcagcctc
     3061 cgcacgctga cgctccgcct cgtcgacggc agcctgctcg gcggccaacc gctcccgctc
     3121 acgacgctcg ccctcgtacc actccgcctc ggcacgctga cgctcggcgg ccacgcgctc
     3181 cgcctccctg cgctgagcct ccagcgctcg ttccgcttcg gcccgttcgg ccgccagccg
     3241 ctcgtcctcg cgttgtgcgg cggcgacgcg ctccgcctcc agtcgttccg cctcgagccg
     3301 ttcccgctcc gcctgggcgg cagccagttc ggcggcggca cgctcggcgg ccagccgttc
     3361 cgcttcggcg cgctccgcct cgagccgctc gcgttcggcc tgctcggcgg cccgtcgctg
     3421 ggcttccagc tccgccgcca gccgctcagc ctcgacccgc tcgcgctctg ccgccgcttc
     3481 ggcgtcgcgg atcgcagcct cacgctcggc cgcgatccgg tccaactcgg cctgctcgac
     3541 cgcagcacgt tcgagttctg cctcctcggc caacaggcgc tgccgctctt cctgctgccg
     3601 tgccgcctcg gactcagcgt cctcaacgaa cagacccgcc tcatcggcga cctgctcagc
     3661 ctccgcgacc gcctcatccg cagcgatgcg ctcagccgca tccgcctcgt ccgcacgctg
     3721 ctcggcctcg accaccgcct cgtccaccgc gacctcgtcg gcccgcttgg ccaccgcggc
     3781 gatctcctcg gcctcctcca ccgcctcgtc ctcggccgcc tcgatccggc cctccgcttc
     3841 ggccgctgcg tggccgacca cccgctcggc gtcgtccacg gcctcggcct cggcgagctc
     3901 gcgctcgatc gcggccaact cggcagcctc ggcagcctcc aagacggcca gttcggcggc
     3961 gagccgttcg agttcctcgc gctcagcccg ctcctggtcg gtctcgtcgt tcaccctcgc
     4021 ccgcagctcc gcctcggcct gggcggcctc gatggccgcc agccgctcgt gctcggcgat
     4081 ccgctcagcc cggcgtcgcc gggtctcctc acgctcggcc gcggcgaccg cgcgggcctc
     4141 ggcctcggcc agtctcgccg cctcggcctc ggcggctgcc cgacgctgct cctgcgccaa
     4201 ccgctgcgcc gcctgctcgg cggatcgacg ctcctcgatc gccgcctgct cggcccggcg
     4261 ggcgagttct gcgtccacct cgaccctaag ccgctgctcc tcggcatcag ccgggccgcg
     4321 ctgagccgcc agccgttcgg acgcctcgcc gttcgcgccg gtctgcacgc cgaaagcctc
     4381 cccggggggc aggggcgtcc atgacaccgg tgcgccgagc ggagtgcgtt gccgcggcgc
     4441 cggacgttcc tggtcgccct cccccgcaat ggacgcggcc aactcggcca cgtcgccggc
     4501 ctcggccgag cggccgacgg agcgcagatc gtccgccagg tcggccagcg cggtgaccag
     4561 caggggctgc gcctcgtcgg tgcgcaccgc caagccgccg agcaggtcga ccaaccgccg
     4621 gcgcagggtg agcgcgagac ctggagcgcc gatcagcaac acccggtcgg cggcatcgct
     4681 gtagacgccg aacaacggct cgctcaggcg ttgcagcatc gccgccggca cgctcgccgg
     4741 ctgctgcagc agctcacgcg cctgcgtgtc gtagcgctcc accgcgcgca gttcgtactc
     4801 gacggacggc tgcggccggc ccgccgccca gcgagcgtcc gcgaccatcc gatcgatgtc
     4861 gaccggaagc caaccctcga ggtcggcggc cacggacgcc gccgcatccg cgcgggcatt
     4921 ggccgtcgcc ggctcccctt gagccagggc gacgcgggcc caggtgagca gcgcccagcc
     4981 gatggcggcg ggcggcaggt cggcgtccag ttccagtcgg ctcaactggc cgcgcaggcc
     5041 aagggctgcc gccagcgtct gcccggcacc ctcggtcaat cctgccgccg actggatcac
     5101 ggccaggtgc accagcgccg tgatcgagcg gacggcgtcc gcccgtggcg tataggggcc
     5161 gacgggcccg tgcgtggcga gcaccttggc gctgaggacc acggcctcct gcgcggacgg
     5221 cgccgcctcg ccccactcgt gcccggcgag ccggtgcgcg gtgagttcga ccagcgcgtc
     5281 cgccaccgct ccgagccgcg tcgggtcgcc gcctgcccag tcgcgcagcc gctgcacctc
     5341 cgaggtcagt cggcgggtgc gttgggcaaa ggtgtctgcg ggctggttca tgagctcctc
     5401 gggtcgctgg ccgcgatgat gttgctgggc atcgcaattg caccatgccc gatcaaccgc
     5461 cacgccggta ccacccctgg cggacgatcg cggacgcgag ccgcaccgat cgaagacgct
     5521 gccgagcgcc actgcacagt acttcccggg tcgcaccgcg tccggtcggc agaccccggg
     5581 tcgcaccgcg tccgctcagc aggcccggcg ccgagcccgg ggcggaggcc tcctccgcgg
     5641 caccggcctc gccttcacta tgatcatgct tcgccgcacc cacccgctgc accaggagcg
     5701 tcgccacatg ctgagccagt tcggtccgag cacctgggcc gcgttgctgt tcggcggtgt
     5761 cctggcggtg ctcgccttcg ttccggtggc cgcctaccgc taccggcgag ccggacggct
     5821 gcgcgcgctc gacatcgtgg tgctgctgat cgtcgccgtc tacgccgtcg ccctgtggag
     5881 ctacacgctg gtgccggtgc ccgagaatga ctcctttcgc tgcgtcggtc acaacctgcg
     5941 gcccttcgcc ttcgtggacg acgtgctggc cggaccccgc tcgctgttgg ccaaccgggc
     6001 ctttctgcag gccgcgttca acgtcgtgct cttcgccccg ttcggcttct ttctgcgcgt
     6061 actgcggcgg cgcggattcg tggtggccac gctggccggg ttcggcacgt ccctggccat
     6121 cgaactcacc cagctgaccg gggtgtgggg catctatcac tgcgcctacc gggtcttcga
     6181 cgtcgacgac ctgctgctca acaccaccgg ggcactgctg ggctcgctgg cgggcctgcc
     6241 ggtgcggcgg ttgttgagcc ggcggcgtcc gttgcccgcg gtgaccacgg tgacgatcgg
     6301 acgtcggttg accggcatgg cgatcgacgt gctggtttgt ggactggtgg gcttcaccgt
     6361 cacggtcggg tggcgtgcga tcgggctgta cgtgctcggc attcccttcg accagctgcc
     6421 cggatggatc gatcagacgc tgctgagcgg cgtcccggcg gtcctggagt gctactgggt
     6481 cctgatgcgg ggacgcacca tcggcgagga cgtggtgcaa ctcgaacccg tcgtgcggcg
     6541 cagcagcccg gcgttggcca gagccgtcaa gtacgtgacc ggggtgggcg gctacctgct
     6601 gttgaacagc gtcctggtgc cgtggccgtt cgcggtgccg ctgttcgccc tggtcacgat
     6661 cgggtgggcg ctcaggtcgc ggcagcaccg gggtatgagc cacaccctcg caggcatgga
     6721 actgcggatc gagcaggcgg ccgaccccgt cggcacgaag ggcaccggct gatcagcggt
     6781 cctccgggcg atgcccagat aacggttggg tcacgattca acgcccggca acggtcccca
     6841 cggctcagag cggtcggtcg acacaaagtc gaagctgaga ttcgttgact cgttcctcag
     6901 cggctccgta gcctcacccc gagggcgtca cctctgcgcc ccagatcctt tgtccagaaa
     6961 ggttgggcgc atcttgctac gccgcaaact cctgatcgtc gcgggcgcac tcgccctgac
     7021 gacaccaacc ctggcctacg ccgacaccgg cgtggacagg ttcgacgagg ccccatccaa
     7081 ggccaagctc gacaccacgt tcgtgcccgc ttcgctcaac cgtgacgcca aggtcaccgt
     7141 catggtcgag atgaccggtg atccggtcgc cgtcgtcgag gccaatacgg acgggcagtt
     7201 gtccgacgcc cgcgcgggcg agatcaggtc cgggctcaaa aagtcccagg accgggtcaa
     7261 gtccgaaatt caggatcgtg gtggcaaggt cctttcgcag atgcaatccg ccttcaacgg
     7321 catgcgggtg accctgcccc gcaagcaggt cgctgccgtg tcgcaactgg acggcgtcaa
     7381 gtccgtcatg gcggccccgg tgcacaccat cgacaacacg gtcagcgtgc cgttcctcgg
     7441 cgtgccgggc gtctggcagc acaccggcta cctcggccag ggcgtcaagg tggcgatcat
     7501 cgacaccggc atcgactaca cccacgccga cttcggcggc gcgggcaccg aggcggcgtt
     7561 caaggcggcc aaggccagca ccaccttcac cccgaccgtg cgggtcaagg gcggctggga
     7621 cttcgtcggc gacgactaca acgccgatcc gagcgccgac gactaccagc cggtgccgca
     7681 tcccgacgcc aacccgctcg actgccaggg ccacggcagc cacgtcgcgg gcaccaccgg
     7741 cggcagcggc gtcaacgagg acggcaccac ctacgccggc ccgtacgaca ccaacaccaa
     7801 gaacaagaag ttcaaggtcg gccccggcgt cgcgcccaag gtggacctgt acgcgctcaa
     7861 ggtcttcggc tgcac
//
LOCUS       DAOSUG010000065         7836 bp    DNA     linear   ENV 10-JUN-2024
DEFINITION  MAG TPA_asm: Micropruina sp. isolate AMR_MDS_2195
            NODE_4700_length_7836_cov_5.222851, whole genome shotgun sequence.
ACCESSION   DAOSUG010000065 DAOSUG010000000
VERSION     DAOSUG010000065.1
DBLINK      BioProject: PRJNA925477
            BioSample: SAMN32864262
            Sequence Read Archive: SRR4236673
KEYWORDS    WGS; ENV; Metagenome Assembled Genome; MAG; Third Party Data; TPA;
            TPA:assembly.
SOURCE      Micropruina sp. (wastewater metagenome)
  ORGANISM  Micropruina sp.
            Bacteria; Bacillati; Actinomycetota; Actinomycetes;
            Propionibacteriales; Nocardioidaceae; Micropruina.
REFERENCE   1  (bases 1 to 7836)
  AUTHORS   Abdulkadir,N., Saraiva,J.P., Zhang,J., Stolte,S., Gillor,O.,
            Harms,H. and Rocha,U.
  TITLE     Genome-centric analyses of 165 metagenomes show that mobile genetic
            elements are crucial for the transmission of antimicrobial
            resistance genes to pathogens in activated sludge and wastewater
  JOURNAL   Microbiol Spectr 12 (3), e0291823 (2024)
   PUBMED   38289113
REFERENCE   2  (bases 1 to 7836)
  AUTHORS   Abdulkadir,N.
  TITLE     Direct Submission
  JOURNAL   Submitted (24-MAR-2023) Environmental Microbiology, Helmholtz
            Center for Environmental Research -UFZ, Permoserstr 15, Leipzig
            04318, Germany
COMMENT     The annotation was added by the NCBI Prokaryotic Genome Annotation
            Pipeline (PGAP). Information about PGAP can be found here:
            https://www.ncbi.nlm.nih.gov/genome/annotation_prok/
            
            ##Genome-Assembly-Data-START##
            Assembly Method        :: SPAdes v. 3.11.1
            Genome Representation  :: Full
            Expected Final Version :: Yes
            Genome Coverage        :: Not Applicable
            Sequencing Technology  :: Illumina MiSeq
            ##Genome-Assembly-Data-END##
            
            ##Genome-Annotation-Data-START##
            Annotation Provider               :: NCBI
            Annotation Date                   :: 05/04/2023 04:57:10
            Annotation Pipeline               :: NCBI Prokaryotic Genome
                                                 Annotation Pipeline (PGAP)
            Annotation Method                 :: Best-placed reference protein
                                                 set; GeneMarkS-2+
            Annotation Software revision      :: 6.5
            Features Annotated                :: Gene; CDS; rRNA; tRNA; ncRNA
            Genes (total)                     :: 2,776
            CDSs (total)                      :: 2,750
            Genes (coding)                    :: 2,714
            CDSs (with protein)               :: 2,714
            Genes (RNA)                       :: 26
            tRNAs                             :: 24
            ncRNAs                            :: 2
            Pseudo Genes (total)              :: 36
            CDSs (without protein)            :: 36
            Pseudo Genes (ambiguous residues) :: 0 of 36
            Pseudo Genes (frameshifted)       :: 2 of 36
            Pseudo Genes (incomplete)         :: 32 of 36
            Pseudo Genes (internal stop)      :: 2 of 36
            ##Genome-Annotation-Data-END##
FEATURES             Location/Qualifiers
     source          1..7836
                     /organism="Micropruina sp."
                     /mol_type="genomic DNA"
                     /submitter_seqid="NODE_4700_length_7836_cov_5.222851"
                     /isolate="AMR_MDS_2195"
                     /isolation_source="Activated sludge of municipal
                     wastewater treatment plants"
                     /db_xref="taxon:2737536"
                     /environmental_sample
                     /geo_loc_name="Canada"
                     /lat_lon="49.25 N 123.1 W"
                     /collection_date="2015-06-16"
                     /metagenome_source="wastewater metagenome"
                     /note="metagenomic"
     gene            complement(<1..1365)
                     /gene="dnaA"
                     /locus_tag="PLF56_03445"
     CDS             complement(<1..1365)
                     /gene="dnaA"
                     /locus_tag="PLF56_03445"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_014845165.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_function: GO:0003688 - DNA replication origin binding
                     [Evidence IEA];
                     GO_function: GO:0005524 - ATP binding [Evidence IEA];
                     GO_function: GO:0043565 - sequence-specific DNA binding
                     [Evidence IEA];
                     GO_process: GO:0006275 - regulation of DNA replication
                     [Evidence IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="chromosomal replication initiator protein DnaA"
                     /protein_id="HOQ52650.1"
                     /translation="MPEQPATPPSIPVADLDDAWRFVWSEADAPSRAWLKNTHAETMH
                     DSTVIISVPNEFTRTRVESRLRGWVEGQLTSYFGRPVGIALVIATTDEEADDDDFELS
                     PSSTHPAPALSSVTEPDERSGGRLNPKYTFESFVIGSSNRFAHAAAVAVAEAPGKAYN
                     PLMIYGESGLGKTHLLHALGHYVRNYHANRRVKYVSTEELTNDFINAISDNRTADFRR
                     TYRDVDVLLIDDIQFLESKIQTQEEFFHTFNTLHTAQKQIVITSDRPPKSLEALEPRL
                     RSRFEWGLMTDIQPPDLETRIAILRKKAAAERLTAGPDVLEFIASKIQANIRELEGAL
                     IRVTAFASLNRQEVDLPLAEVVLRDLIPHGDDTPITATLIQSETAAYFSITTDDLCGT
                     SRVQSVVMARQMAMYLCRELTDLSLPKIGQSFGGRDHTTVMHANKKIRHLMGEDRKVF
                     NQVAE"
     gene            1695..1832
                     /gene="rpmH"
                     /locus_tag="PLF56_03450"
     CDS             1695..1832
                     /gene="rpmH"
                     /locus_tag="PLF56_03450"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_013428281.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_component: GO:0000315 - organellar large ribosomal
                     subunit [Evidence IEA];
                     GO_component: GO:0022625 - cytosolic large ribosomal
                     subunit [Evidence IEA];
                     GO_function: GO:0003735 - structural constituent of
                     ribosome [Evidence IEA];
                     GO_process: GO:0006412 - translation [Evidence IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="50S ribosomal protein L34"
                     /protein_id="HOQ52651.1"
                     /translation="MSKRTFQPSNRRRSRTHGFRLRMRTRAGRAIISARRSRGRARLA
                     G"
     gene            1855..2187
                     /gene="rnpA"
                     /locus_tag="PLF56_03455"
     CDS             1855..2187
                     /gene="rnpA"
                     /locus_tag="PLF56_03455"
                     /EC_number="3.1.26.5"
                     /inference="COORDINATES: protein motif:HMM:NF013022.2"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_function: GO:0004526 - ribonuclease P activity
                     [Evidence IEA];
                     GO_process: GO:0006396 - RNA processing [Evidence IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="ribonuclease P protein component"
                     /protein_id="HOQ52652.1"
                     /translation="MLAQAERLRTADEFRTTVRRGARSARPTLVLHMLRTDTPPSRAG
                     FVVSKAVGNAVRRNRVKRQLRHLVAARLHETPFSCDVVVRALPRPGALSDDLARAWSS
                     SVTRLARP"
     gene            2184..2462
                     /gene="yidD"
                     /locus_tag="PLF56_03460"
     CDS             2184..2462
                     /gene="yidD"
                     /locus_tag="PLF56_03460"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_007079292.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="membrane protein insertion efficiency factor
                     YidD"
                     /protein_id="HOQ52653.1"
                     /translation="MKTVLIGLLKAYRALISPLYGPVCKYYPSCSAYALEAVQVHGAR
                     RGAGLALRRLGHCHPWSLGGYDPVPGTPAAAQWAAEQRLTLNEIRGAI"
     gene            2462..3586
                     /gene="yidC"
                     /locus_tag="PLF56_03465"
     CDS             2462..3586
                     /gene="yidC"
                     /locus_tag="PLF56_03465"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_013866181.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_function: GO:0032977 - membrane insertase activity
                     [Evidence IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="membrane protein insertase YidC"
                     /protein_id="HOQ52654.1"
                     /translation="MPPIPQLVPLDLWSDFLGALNTMMQPLYWAVSWIMSSFHSLYAS
                     FLGADNGWSWALAIISLTVVIRTALIPLFVRQIRSSRNMQILQPQLKALQEKYGHDRE
                     RLGQETMKLYKDEGVNPMASCLPLVLQMPIFIALYQVLWQSSQGHAIGVMSVEQAESL
                     RNATILGARLSGTFLPMTDGFGATQVLCAVLVISMTAVLFVTQLQLMRKNMPPEALTG
                     QFAQQQKIMLYVFPVMYLFMGINIPIGVLLYWLVSNLWTMTQQYILIHNNPAPNTPAY
                     IDWEERMRARGKDPDELARKRAGKGRKTAPAPTGDATKVSRQGATGTATKSGADGADA
                     PGVQPQQVQRQQVQRQQPQRSSRSARKTGPAKPKSTTGDV"
     gene            3610..4194
                     /locus_tag="PLF56_03470"
     CDS             3610..4194
                     /locus_tag="PLF56_03470"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_015072078.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_function: GO:0003676 - nucleic acid binding [Evidence
                     IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="R3H domain-containing nucleic acid-binding
                     protein"
                     /protein_id="HOQ52655.1"
                     /translation="MTDLHIDEASSPEGAVEIDTDVTATEADAQSDDAESEHNRSKRE
                     QALDNEGEIAADYLEELLDIADLDGDIDTFSEGGRAHVSIVTDSDVLVGRNGEVLEAL
                     QELARLAVMTETGHRSRLMLDVAGHRDKRRKELEVLAGDAITEVVDGGEPVRLAPMNP
                     FERKIVHDVVAAAGLVSESEGEEPRRRVVVLPKG"
     gene            4512..5060
                     /gene="rsmG"
                     /locus_tag="PLF56_03475"
                     /pseudo
     CDS             4512..5060
                     /gene="rsmG"
                     /locus_tag="PLF56_03475"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_002544051.1"
                     /note="incomplete; partial in the middle of a contig;
                     missing N-terminus; Derived by automated computational
                     analysis using gene prediction method: Protein Homology.
                     GO_component: GO:0005575 - cellular_component [Evidence
                     IEA];
                     GO_function: GO:0008168 - methyltransferase activity
                     [Evidence IEA];
                     GO_process: GO:0031167 - rRNA methylation [Evidence IEA];
                     GO_process: GO:0046118 - 7-methylguanosine biosynthetic
                     process [Evidence IEA]"
                     /pseudo
                     /codon_start=1
                     /transl_table=11
                     /product="16S rRNA (guanine(527)-N(7))-methyltransferase
                     RsmG"
     gene            5835..6746
                     /locus_tag="PLF56_03480"
     CDS             5835..6746
                     /locus_tag="PLF56_03480"
                     /inference="COORDINATES: protein motif:HMM:NF022077.2"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_function: GO:0005524 - ATP binding [Evidence IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="ParA family protein"
                     /protein_id="HOQ52656.1"
                     /translation="MTQSAVPRRIAYDEDEFAIVTADLPSPTVPRETVPLPVPGSTRV
                     FVIANQKGGVGKTTSAVNIAAALARGGLRVLVIDLDPQGNASTALGIDHEAGVAGTYE
                     VLIDGAPIEEHVQSSPEADNLWVLPATIDLAGAEIGLVDVRGRESRLLRALDQLLAVH
                     SLDYVFIDCPPSLGLLTLNALVAAEELLVPIQCEYYALEGVSQLVRTIDLVKGSMNDR
                     LRLSTIVLTMYDARTRLSAQVAAEVRAHFATETLRTAIPRSVRVSEAPSYGRTVITYD
                     QASAGSQAYLSVAEEIANRSIADRVRA"
     gene            6743..7663
                     /locus_tag="PLF56_03485"
     CDS             6743..7663
                     /locus_tag="PLF56_03485"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_016668684.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_function: GO:0003677 - DNA binding [Evidence IEA];
                     GO_process: GO:0000910 - cytokinesis [Evidence IEA];
                     GO_process: GO:0007059 - chromosome segregation [Evidence
                     IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="ParB/RepB/Spo0J family partition protein"
                     /protein_id="HOQ52657.1"
                     /translation="MNSTKPRPGLGRGLGDLIHRTDPSLEPERAAPLAVEPPQGSYYA
                     DVAVTEIRPNPKQPRQVFDEDALAELVESIREVGLLQPVVVRPLDEGGYELVMGERRL
                     RATQAAGLSTIPAIVRPTQEHDLLRDALLENLHRAQLNPLEEAAAYQQLLTDFDCTQE
                     ELSTRIKRSRPHISNTIRLLQLPAPVQRRVAAGVLSAGHARAILGLGDPLAMERLAQR
                     VVAENLSVRTVEEIVALGDGGQPARTRARVAREPSERASALADRLIERLDTRVNVVMG
                     AKGKGRIVIDFAGEDDLNRVAALLGGDQPS"
BASE COUNT         1279 a         2549 c         2643 g         1365 t
ORIGIN      
        1 ctcggcgacc tggttgaaca ctttgcggtc ctcgcccatc aggtggcgga tcttcttgtt
       61 ggcgtgcatc accgtggtgt ggtcgcggcc gccgaaggac tggccgatct tgggcagcga
      121 caagtcggtg agctcacgac acaggtacat ggccatctgc cgggccatca cgacgctctg
      181 cacgcggctg gtgccgcaca gatcgtcggt ggtgatcgag aagtacgccg cggtctcgct
      241 ctggatcagg gtcgcggtga tcggggtgtc gtcgccgtgc ggaatcaggt cgcgcaacac
      301 cacctcggca agcggcaggt cgacctcctg ccggttcagg ctggcgaacg cggtgacccg
      361 gatcagggcc ccttcgagct cacggatgtt ggcctgaatc ttgctggcga tgaactcgag
      421 cacgtcgggg ccggcggtca gccgctcggc cgccgccttc ttgcgcagga tcgcgatgcg
      481 ggtctcgagg tcgggcggtt gaatatctgt catcagaccc cactcgaatc ggctgcgcag
      541 tctcggctcg agggcctcca acgacttggg cggacgatcc gaggtgatga cgatctgctt
      601 ctgcgcggtg tgcagggtgt tgaacgtgtg aaagaactct tcctgggtct ggatcttgct
      661 ctccaggaac tggatgtcgt cgatcagcag cacgtccacg tcgcggtagg tgcgccggaa
      721 gtcggcggtg cggttgtcgc tgatcgcgtt gatgaagtcg ttggtcagct cttcggtcga
      781 tacgtacttg acccggcggt tggcgtgata gttgcgcaca tagtggccga gcgcgtgcag
      841 caggtgggtc ttaccgaggc ccgattcgcc gtagatcatc agcgggttgt acgccttgcc
      901 cggcgcttcg gccacggcca ccgcggcggc gtgcgcgaac cggttcgagc tgccgatcac
      961 gaacgattcg aacgtgtatt tcgggttgag ccggccgccc gatcgctcgt ccggctcggt
     1021 gaccgaactc aacgccggcg cggggtgggt gctggacggc gacaactcga aatcgtcgtc
     1081 gtccgcttct tcgtcagtcg tggcgatcac cagcgcaatg ccgaccggac gtccgaagta
     1141 gcttgtcagc tgcccctcga cccacccgcg cagccgcgat tcgacccggg tgcgggtgaa
     1201 ttcgttgggc acgctgatga tcacggtgct gtcgtgcatg gtctcggcgt gggtgttctt
     1261 cagccaggcc ctggacgggg cgtcggcctc ggaccagacg aagcgccagg cgtcgtccag
     1321 gtcggcgacg ggaatgctcg gaggagtcgc cggttgctcg ggcacctgcg ctcccttcct
     1381 gcgtactcaa cgggtcgatc gaatcgtcca cacaagtttt ccacagggtg tgaacaaggg
     1441 ataatggtcc gaggctggac agcccaaggt caacggagcg aaacgctagc cccccggacg
     1501 cgcactccgc aaacgttcgc tgagaaaagt tggacgtgct cggcgtgtcg gctcgacgca
     1561 gcacgaatgg gcactggttt gccccgggtc ggggtggccg cgtaaccttg gcaggtcgcc
     1621 gccagcgatt cacctgcgtg cgtccggagt gggcgcatcg gttccagcta gatcttgaaa
     1681 ccaggagcac ttccgtgagc aagcgcactt tccagccgag caaccggcgt cgcagccgca
     1741 cccatggctt tcgcctgcgc atgcgcaccc gcgccggccg cgccatcatt tcggctcgcc
     1801 gtagccgggg ccgcgcccgt ctcgccggct gatcagcacc tgacgagtcg tcaggtgctg
     1861 gctcaggccg agcggctgcg aaccgcagac gagttccgca ccactgtgcg tcggggcgcc
     1921 cgtagcgcgc gccccactct ggtgctgcac atgctgcgca ccgacacccc gccgtcccgg
     1981 gcgggttttg tggtatccaa ggcggtgggc aacgcggtgc gccgcaaccg cgtcaagcgg
     2041 cagttgcgcc atctcgtcgc tgcccgcctg cacgagacgc cgttctcttg cgatgtggtg
     2101 gtgcgggccc tgccgcggcc aggagccctg tcggacgatc tggcgcgcgc ctggtcgtcc
     2161 agcgtcacac gtctggcgcg cccatgaaga cagtgctgat cggcctactg aaggcctacc
     2221 gggcgctgat cagcccgctg tacgggccgg tctgcaagta ctacccgagc tgttcggcct
     2281 acgcgctgga ggcggtccag gtgcatggcg cccggcgcgg cgcgggcctg gcgctgcgcc
     2341 gactcgggca ctgtcacccg tggtcgctcg gtgggtatga cccggtaccg ggcaccccgg
     2401 cagcggcgca gtgggcggcc gagcaacgcc tcacactcaa cgaaatccga ggagcaatct
     2461 gatgccaccg attccacagc ttgtcccgct ggacctgtgg tccgacttcc tcggtgcgct
     2521 caacaccatg atgcagccgc tctactgggc ggtctcgtgg atcatgagca gcttccactc
     2581 cctctacgcg tcgttcctgg gcgccgacaa cggctggtcg tgggcgctgg cgatcatctc
     2641 gctgaccgtg gtcatccgca ccgcgctgat tccgctgttc gtgcggcaga tccgcagttc
     2701 gcgcaacatg cagatcctgc agccccaact gaaggcgctg caggagaagt acggccacga
     2761 tcgcgagcgc ctcggtcaag agaccatgaa gctgtacaag gacgagggcg tcaacccgat
     2821 ggcgtcctgc ttgccgctgg tgctgcagat gccgatcttc atcgccctgt accaggtgct
     2881 gtggcagtcc tcgcaggggc acgcgatcgg cgtgatgagc gtcgagcagg ccgagtcgct
     2941 gcgcaacgcc acgattctcg gggcccgact gtcgggcacc ttcctgccga tgaccgacgg
     3001 gttcggcgcc actcaggtgc tgtgcgcggt gctggtgatc agcatgaccg cggtcctgtt
     3061 cgtgacccag ttgcagctga tgcgcaagaa catgccgccg gaggcgctca ccggccagtt
     3121 cgcccagcag cagaagatca tgctgtacgt cttcccggtg atgtacctgt tcatgggcat
     3181 caacattccg atcggagtgc tgctgtactg gctggtctcg aacctgtgga cgatgacgca
     3241 gcaatacatc ctgattcaca acaacccggc gccgaacaca cctgcctaca tcgactggga
     3301 agagcgcatg cgggcccggg gcaaggatcc cgacgaactg gcgcgcaagc gggcaggcaa
     3361 ggggcgcaag accgccccag cccccaccgg cgatgcgacg aaggtgtccc ggcagggtgc
     3421 gaccggtacg gcgaccaagt ccggtgctga tggagcggac gcgcccggcg tccagcctca
     3481 gcaggtgcag cgccagcagg tgcagcggca gcagccgcaa cgctcaagcc gctccgcgcg
     3541 caagaccggg cctgcgaagc caaagtccac caccggcgac gtctgagccg acgaaggaag
     3601 agaccccaga tgactgatct gcacatcgac gaagcaagct cccccgaagg cgcggtcgag
     3661 atcgatactg acgtgaccgc gaccgaagct gacgcgcagt ccgacgacgc cgagtcggag
     3721 cacaaccggt ccaagcgtga gcaggcactc gacaacgagg gcgagatcgc ggccgactac
     3781 ctcgaagaac tgctcgacat cgccgatctg gacggcgaca tcgacacctt cagcgagggt
     3841 ggccgggcgc acgtatcgat cgtgaccgat tcggacgtgc tggtcggccg caatggtgaa
     3901 gtgctcgagg cactgcaaga actggcgcgg ctggccgtga tgaccgagac cggtcaccgc
     3961 tcccggttga tgctcgacgt cgccggccat cgcgacaagc ggcgcaagga actcgaggtg
     4021 ctggccggcg acgccatcac cgaggtggtc gatggcggcg agcctgtccg gttggccccg
     4081 atgaatccgt tcgaacgcaa gatcgtccac gatgtcgtgg cggccgcggg cctggtcagc
     4141 gagtccgagg gcgaagagcc tcgtcgacgc gtggttgtgc tgcccaaggg ctgacccgac
     4201 tgcagctgcg aaaccgagtc gagccggatg cccccgggcg tccggctcga ccgttctctg
     4261 tacgagagtt cgcctgggtc gtcgcgaggc gattgtcgaa tgacgacgcc gggcctaccg
     4321 gcggctcagg cgtgcgtacg atcaccaggg tctcgatcag cgctggatcg gcgtgctgcc
     4381 tcgtggtgtg tcgctcaaat ggcaagtgat tcggggacac gcgaccagtt ggtgtcacgg
     4441 ctccggtgca gtagacaggt gggatgcaga tcgctcgtga ggtgtttggc gacaggtatg
     4501 acctcgctgg gcaatatatc gacatattgt ctagtcgagg agtcgactgg ggcctgatcg
     4561 ggccgcgtga ggtgagtcgg ttgtggcccc gtcacgttct gaactccgtg gctgtgagca
     4621 gtctcatcga gccgtcggcc gacgtgctcg acgtgggctc gggcgcggga ctgcccggca
     4681 ttcccttggc gatcctgcgg ccggacctca ctgtcaccct gctcgagcca ctgctgcgtc
     4741 gcttcaactt tctgaccgag gcggtgcgcg agttggggct ggacgagcag gtgcgggtgg
     4801 tgcgcggacg ggccgaggag cagcggtcaa cgtaccaggt ggtgacgtgc cgggcggtcg
     4861 ccccgcttcc gaaactgctc ggctggtgcc tacccctggt gcgtccgcac gggcaactgc
     4921 tggccatcaa gggcgagaac gccgagcagg agctactgga cgtgcagggt gtgctgcgat
     4981 cccgtcggtt gacgggtgag gtgctgttgg tccgcgccga tccagctgct gagccgacct
     5041 gggtggtgcg cattcgctga ccgtcggtca tcccggcgcc gggatgtcgt cctccctcgt
     5101 cgtccggcgc gtgccgggat gtcttccggc gcgtgccggg atgtcgtccc ccccgtcatc
     5161 ccggcgaatg ccgggatctc gtcctccccg tcgtcccggc gcgtgccggg atctcgtcct
     5221 ccccgtcgtc ccggcgcgtg ccgggatgtc gtcctccccc gtcatcccgg cgcatgccgg
     5281 gatctcgtcc gccgcagtcg tcccggcgca tgccgggatc tctgggtgtg ctgagatccc
     5341 gcggctagcc ccggctagtc ccggctagtc ccggctagcc ccggctagcc ccggcattcg
     5401 gcgacgcctc tggtcgaact catcgggaca aagcgaccgg ggcttcaaac aagctcaggg
     5461 gtcgccattg atggcgctcc cccagcccct ggcctggact cagccacggc gaaggcgcgt
     5521 agcgcgagtc gacccggcta gacccggcta gaccgggaca tatacgctcc cctaccgggc
     5581 ctatctctgc ggcgggtgat tcggtgccca cactggcaag tccgtccgac ccgctccgcg
     5641 cacccgcaac cggctacagg agcgatgcga gcgcactcgc ctctcagact cgctgccgat
     5701 gtacccggtg ttggctttgc acaccgatag ggtcggtaat cgacgtcgta cgagcgcgaa
     5761 gaggttgcag ctgtgctggt ttgtcgaact ttgctggttt cacgtgaaac agccgtcggt
     5821 cagcgggtgc accgatgact cagtcagcgg tcccccgccg gatcgcctac gacgaggacg
     5881 agtttgccat cgtgacggcc gatttgccct cccccactgt tccacgtgaa acggttccgc
     5941 tccccgtacc cggctccacc cgcgtcttcg tcatcgccaa tcagaagggt ggggtcggca
     6001 agaccacgtc ggcggtgaac atcgctgccg cactcgcccg aggcgggctc cgggtgctgg
     6061 tgatcgacct cgacccccag ggcaacgctt cgacggcgct gggcattgat cacgaggccg
     6121 gcgtcgccgg tacctatgag gtgctgatcg acggtgcacc gatcgaagag cacgtgcagt
     6181 cctcgcccga ggccgacaac ctgtgggtgc tgcccgccac gatcgacctg gccggtgcag
     6241 aaatcggtct ggtcgacgtg cgaggtcgcg agagccgcct actgcgggcc ctcgatcagc
     6301 tactggctgt gcacagtctt gactacgtct tcatcgactg ccctccctcc ctcggcctcc
     6361 tcaccctcaa cgcgttggtc gcggccgaag aactgctggt gcccattcag tgcgagtact
     6421 acgccctcga gggagtgtca cagctggtgc gcaccatcga cctggtgaag ggctccatga
     6481 atgaccgact gaggctctcg acgatcgtgc tcaccatgta tgacgcgcgc acccgactct
     6541 cggcacaggt cgcggctgag gtgcgcgccc acttcgcgac cgagacactg cgcacggcga
     6601 tcccccgttc ggtacgagtg tccgaagcgc caagctatgg acggacggtg atcacctatg
     6661 atcaggcctc cgccggatcg caggcgtacc tttccgtagc cgaagagatc gcgaaccgca
     6721 gcattgcaga cagggtgcgc gcatgaactc gacgaaaccc cgtcccggct tgggccgtgg
     6781 cctcggcgac ctgattcacc gcaccgaccc gagcctggaa ccggagcgcg cggcgccgct
     6841 ggccgtcgag ccaccgcagg gcagctacta cgcggatgtc gccgtcaccg agatccgtcc
     6901 caaccccaag cagccccgcc aagtgttcga cgaggacgcc ctggccgaac tcgtcgagtc
     6961 gatccgcgag gtggggttgc tgcaacccgt ggtcgtgcgg ccattggacg agggcggcta
     7021 cgaactggtg atgggggagc ggcgtctgcg cgcaacgcag gccgcggggc tgtcgaccat
     7081 tccggccatc gtccggccga cccaggagca cgacctgctg cgtgacgcgt tgctcgagaa
     7141 cctgcaccgg gcccagttga acccgctgga agaggcggcg gcctatcaac agttgctgac
     7201 cgacttcgac tgcacccaag aagaactctc gacccgcatc aagcgcagtc gtcctcatat
     7261 cagcaacacc atccggttgc ttcagttgcc agcgccggtt caacgtcggg tcgccgccgg
     7321 ggtgctgagc gccgggcatg cccgagcaat tctgggtctg ggcgatccac tggcgatgga
     7381 gcgtctcgcg caacgggtgg tggcagaaaa cctgtcggtg cgtaccgtcg aagagatcgt
     7441 tgcgctgggc gacggcggtc agccggcgcg cactcgtgcg cgcgtggcgc gcgagccgag
     7501 tgagcgggcg agcgcgctgg ccgaccggtt gatcgagcgg ttggacacca gagtcaatgt
     7561 cgtgatgggc gccaagggca agggacgcat cgtgatcgac ttcgcgggcg aggacgacct
     7621 gaatcgcgtg gccgcgctgc tcggtggcga tcaaccgagc taggaaagag ccgaccaacg
     7681 cgatttgttg attgagcggg tcggaaccca ggagcgatca ggtcgcgagc cggtcgagcg
     7741 cagtcttgga cgcgctcgta caggttggac gaggccgggc ttggacgcgc tcgtacaggt
     7801 tggacgagcc cgggcttgga cgcgctcgta cagttt
//
LOCUS       DAOSUG010000066         7835 bp    DNA     linear   ENV 10-JUN-2024
DEFINITION  MAG TPA_asm: Micropruina sp. isolate AMR_MDS_2195
            NODE_4702_length_7835_cov_4.213882, whole genome shotgun sequence.
ACCESSION   DAOSUG010000066 DAOSUG010000000
VERSION     DAOSUG010000066.1
DBLINK      BioProject: PRJNA925477
            BioSample: SAMN32864262
            Sequence Read Archive: SRR4236673
KEYWORDS    WGS; ENV; Metagenome Assembled Genome; MAG; Third Party Data; TPA;
            TPA:assembly.
SOURCE      Micropruina sp. (wastewater metagenome)
  ORGANISM  Micropruina sp.
            Bacteria; Bacillati; Actinomycetota; Actinomycetes;
            Propionibacteriales; Nocardioidaceae; Micropruina.
REFERENCE   1  (bases 1 to 7835)
  AUTHORS   Abdulkadir,N., Saraiva,J.P., Zhang,J., Stolte,S., Gillor,O.,
            Harms,H. and Rocha,U.
  TITLE     Genome-centric analyses of 165 metagenomes show that mobile genetic
            elements are crucial for the transmission of antimicrobial
            resistance genes to pathogens in activated sludge and wastewater
  JOURNAL   Microbiol Spectr 12 (3), e0291823 (2024)
   PUBMED   38289113
REFERENCE   2  (bases 1 to 7835)
  AUTHORS   Abdulkadir,N.
  TITLE     Direct Submission
  JOURNAL   Submitted (24-MAR-2023) Environmental Microbiology, Helmholtz
            Center for Environmental Research -UFZ, Permoserstr 15, Leipzig
            04318, Germany
COMMENT     The annotation was added by the NCBI Prokaryotic Genome Annotation
            Pipeline (PGAP). Information about PGAP can be found here:
            https://www.ncbi.nlm.nih.gov/genome/annotation_prok/
            
            ##Genome-Assembly-Data-START##
            Assembly Method        :: SPAdes v. 3.11.1
            Genome Representation  :: Full
            Expected Final Version :: Yes
            Genome Coverage        :: Not Applicable
            Sequencing Technology  :: Illumina MiSeq
            ##Genome-Assembly-Data-END##
            
            ##Genome-Annotation-Data-START##
            Annotation Provider               :: NCBI
            Annotation Date                   :: 05/04/2023 04:57:10
            Annotation Pipeline               :: NCBI Prokaryotic Genome
                                                 Annotation Pipeline (PGAP)
            Annotation Method                 :: Best-placed reference protein
                                                 set; GeneMarkS-2+
            Annotation Software revision      :: 6.5
            Features Annotated                :: Gene; CDS; rRNA; tRNA; ncRNA
            Genes (total)                     :: 2,776
            CDSs (total)                      :: 2,750
            Genes (coding)                    :: 2,714
            CDSs (with protein)               :: 2,714
            Genes (RNA)                       :: 26
            tRNAs                             :: 24
            ncRNAs                            :: 2
            Pseudo Genes (total)              :: 36
            CDSs (without protein)            :: 36
            Pseudo Genes (ambiguous residues) :: 0 of 36
            Pseudo Genes (frameshifted)       :: 2 of 36
            Pseudo Genes (incomplete)         :: 32 of 36
            Pseudo Genes (internal stop)      :: 2 of 36
            ##Genome-Annotation-Data-END##
FEATURES             Location/Qualifiers
     source          1..7835
                     /organism="Micropruina sp."
                     /mol_type="genomic DNA"
                     /submitter_seqid="NODE_4702_length_7835_cov_4.213882"
                     /isolate="AMR_MDS_2195"
                     /isolation_source="Activated sludge of municipal
                     wastewater treatment plants"
                     /db_xref="taxon:2737536"
                     /environmental_sample
                     /geo_loc_name="Canada"
                     /lat_lon="49.25 N 123.1 W"
                     /collection_date="2015-06-16"
                     /metagenome_source="wastewater metagenome"
                     /note="metagenomic"
     gene            complement(<1..950)
                     /locus_tag="PLF56_03490"
     CDS             complement(<1..950)
                     /locus_tag="PLF56_03490"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_002536884.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_function: GO:0016491 - oxidoreductase activity
                     [Evidence IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="FAD-dependent oxidoreductase"
                     /protein_id="HOQ52658.1"
                     /translation="MSAHYDVVVLGAGPGGYVAAIRAAQLGQKVAIIEKQWWGGVCLN
                     VGCIPTKALLRNAELAHIFTHDKELFGIEGDVTFNFGRAFSRSREVSNRMVGGIHYLM
                     KKNKITEINGWGTFTDAKSIEVKTDTGTEVVTFDNVIIAAGSTTRLIPGTTRSARVFT
                     YEEQILSDNLPESIIICGSGAIGTEFAYVLANYGVKVTIVEFLDRMVPLEDPEISREL
                     AKAYKKLGVEVLTSTKVEAIEETDAGVKVTVSPAKGGEQKVLEADRVLQSIGFAPRTE
                     GYGLDKLGVELTERGAIKIDDYMRTNVPGVYAIGDVTMKLA"
     gene            1101..2621
                     /locus_tag="PLF56_03495"
     CDS             1101..2621
                     /locus_tag="PLF56_03495"
                     /EC_number="2.5.1.7"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_013863424.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_function: GO:0016765 - transferase activity,
                     transferring alkyl or aryl (other than methyl) groups
                     [Evidence IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="UDP-N-acetylglucosamine
                     1-carboxyvinyltransferase"
                     /protein_id="HOQ52659.1"
                     /translation="MTELSPTRIGFLIRDARRQRGLTQQELADELGTSPGIVDDLEAG
                     HRALSVELVNKIAEALEQTQPVGGALHLRIRGETKLTGSIAVRSSKNASVALLCACLL
                     NRGRTVLRGIAQIEEVNRILEVLTSIGVRATWSQDRTELELIRPDRLQLDRMDEEAAR
                     RTRSIIMFLGPLLHDETNFELPYAGGCNLGTRTVSPHLHALRQFGLDIKATKGSYHAH
                     VDPIDPSAYRSITLTERGDTVTENVLMAAARHPGETVIRNASSNYMVQDLCFFLQELG
                     VHIDGIGSTTLRVHGRALIDADVAFAPSEDPIEAMSLLTAAIVTGSELTVQRCPIEFL
                     EIELSILTEMGLRYVLSPEYPAENGRTRLVDLTVLPSQLHANSDKIHPMPFPGLNIDN
                     LPFFAVIAAVAEGQTMIHDWVYENRAIHLVELAKLGAKVQLLDPHRIIVTGPTRWRGQ
                     EIISPPALRPAVCLLLAALAARGTSVLRDVYVINRGYEDLPNRLNALGAHIEVFRD"
     gene            2720..3490
                     /gene="map"
                     /locus_tag="PLF56_03500"
     CDS             2720..3490
                     /gene="map"
                     /locus_tag="PLF56_03500"
                     /EC_number="3.4.11.18"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_007079524.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_process: GO:0036211 - protein modification process
                     [Evidence IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="type I methionyl aminopeptidase"
                     /protein_id="HOQ52660.1"
                     /translation="MAIELLSPRQLDEMRPAGAFIAEVLTALVEHAAVGVDLLDLDGL
                     AHDMIRARGATSCYIDYAPSFGRGPFGKVLCTSVNDAVLHGLPHAYRLRDGDLVSFDF
                     AAGVDGWVCDSAVSVVVGTPDQDDLRLIEATEVALARAIAMARPGNKVGDLSAAIGAT
                     AREYGYTVNTQFGGHGVGRTMHGDPHISNDGRPGRGFALRPGLVIAIEPWLMAGTDQI
                     RMDDDGWTIRSADGSRTAHSEHTIAITAGEPIVMTARG"
     gene            complement(3618..4472)
                     /gene="pdxY"
                     /locus_tag="PLF56_03505"
     CDS             complement(3618..4472)
                     /gene="pdxY"
                     /locus_tag="PLF56_03505"
                     /EC_number="2.7.1.35"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_013863428.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_function: GO:0008478 - pyridoxal kinase activity
                     [Evidence IEA];
                     GO_process: GO:0009443 - pyridoxal 5'-phosphate salvage
                     [Evidence IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="pyridoxal kinase PdxY"
                     /protein_id="HOQ52661.1"
                     /translation="MTVILSIQSSVAYGHVGNSAATFPLMRSGIEVWPVLTVHFSNHT
                     GYGQWRGPLLHANDVAEVIQGIDERGVLNRVDAVLSGYQGAEEVGAVVLDAVALVKQR
                     NPQAIYCCDPVMGDVGRGFFVRPGIPEFMRDKVVPQAQIVTPNHFELDYLTGRETHTL
                     DAVIEAAHALRAVGPETVLVTSVVTDATDGDCLTLLAVSGDGAWQVTTPRLDRVFTGS
                     GDLTAAMFLAHQLRTGSVAQALGHTADIVYSVLKLTADSGSSELQLVAAQDEIVTPTY
                     HFDVTQVR"
     gene            complement(4548..4919)
                     /locus_tag="PLF56_03510"
     CDS             complement(4548..4919)
                     /locus_tag="PLF56_03510"
                     /inference="COORDINATES: ab initio
                     prediction:GeneMarkS-2+"
                     /note="Derived by automated computational analysis using
                     gene prediction method: GeneMarkS-2+."
                     /codon_start=1
                     /transl_table=11
                     /product="GatB/YqeY domain-containing protein"
                     /protein_id="HOQ52662.1"
                     /translation="MPDESAPLRHALRDALPGAMKSGDTPGRNAIRAALAAIENAESV
                     PSDQNVTRAATSEHIAGAVVGLGAAEVQRRELTEEQTLEIVSAERDERLAAAAQYRAA
                     GQAERAAQLEAEALALDSFLC"
     gene            5004..5786
                     /locus_tag="PLF56_03515"
     CDS             5004..5786
                     /locus_tag="PLF56_03515"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_013863431.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="glycerophosphodiester phosphodiesterase"
                     /protein_id="HOQ52663.1"
                     /translation="MQARDYRYFDTGFAALAHRGGYAADTDRTRENTLFAFTSAAALG
                     YRYLETDVHVSSDGVLFSFHDDTLDRVTDARGIPERLPWTELRQVRVGGRDPIATMDE
                     LFEALPRARFNIDLKVPGAIEPLARTIEAHRAHDRVCVGSFSPAGIRAFRRLVGNRVA
                     TAVGPWGVAWAGYARGLRRIVRSPGVALQVPARTWHDRVPVLTRGLVRAAHAGGRVVH
                     VWTVNDPAEMHRLIDLGVDGLVSDRIDVLKAVLIERDRWEEN"
     gene            5786..7135
                     /locus_tag="PLF56_03520"
     CDS             5786..7135
                     /locus_tag="PLF56_03520"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_011756113.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="MFS transporter"
                     /protein_id="HOQ52664.1"
                     /translation="MRSTTRQRAWYFYDWANSAYVTTTATVILGPYLTSLATRAACPD
                     LPAGQVCTNPVNLLGLPVLPGAVHPLLITISTIVTLVALLIIGVVADRSPHPQWWLGG
                     LAWVGAAAASAMFFLDGTNWQLGAVLMIIANLCVGSSQVIYDGILVRIAAPDDRDRVS
                     SRGWALGYLGGGLLLALNLVLMTAYDSFGISYGLAIRLSLLSAGLWWGLFTIIPVLGL
                     RSLPPRALDELQQRADVRRNPVRQLAHTFADLRKYPQTLLFLAAYLFYNDGIQTVIGQ
                     SSLYAQSELAMSTSQVMVTFLLTQFVAFGGALLFGRVAARLGAKRTVLIGIGIWFAVV
                     AVAFFLPAGVFVALLALGAAIGLVMGGTQALSRSMYSQLVPKGREAEYFSFYQAMERG
                     TSWLGTLSFALVFQFSQSYRWAIVALVAFFLVGGLLLTRVDMRRGILQAGNELPKVL"
     gene            7207..7539
                     /locus_tag="PLF56_03525"
     CDS             7207..7539
                     /locus_tag="PLF56_03525"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_002548372.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="RNA polymerase-binding protein RbpA"
                     /protein_id="HOQ52665.1"
                     /translation="MAERSLRGSGLGSKSFADGAGVEFAARQETGFDCPKGHHFTVTF
                     AEEAELPTEWECPRCGGVAVRSDGVVAEAKEVKPPRTHWDMLRERRSIEELEELLAER
                     LDEIRGTD"
     gene            complement(7585..>7835)
                     /locus_tag="PLF56_03530"
     CDS             complement(7585..>7835)
                     /locus_tag="PLF56_03530"
                     /inference="COORDINATES: ab initio
                     prediction:GeneMarkS-2+"
                     /note="Derived by automated computational analysis using
                     gene prediction method: GeneMarkS-2+."
                     /codon_start=3
                     /transl_table=11
                     /product="hypothetical protein"
                     /protein_id="HOQ52666.1"
                     /translation="FTDIFGLIFLLPFTRPFARNLLQLFISRRAARAGVNVDVMRAKV
                     ERDTVIRGETVPDAPGPAAGDHQRGPSGPMVIKGEIEP"
BASE COUNT         1229 a         2695 c         2588 g         1323 t
ORIGIN      
        1 gcgagcttca tggtgacgtc gccgatggcg tagacgcccg gcacgttggt gcgcatgtag
       61 tcgtcgatct tgatcgcacc gcgctcggtc agttcgacgc ccagcttgtc gagcccgtag
      121 ccctcggtgc ggggggcgaa gccgatcgac tgcagcaccc ggtcggcctc gagcaccttc
      181 tgctcgccgc ccttggcggg actcacggtc actttgacgc ccgcatcggt ctcttcgatc
      241 gcctcgacct tggtggaggt gagtacctcg acgccgagct tcttgtacgc cttggccagt
      301 tcacgcgaga tctcggggtc ttcgagcggc accatacgat cgagaaactc gacgatcgtg
      361 accttgacgc cgtagttggc cagcacgtag gcgaactcgg tgccgatggc gcccgagccg
      421 cagatgatga tcgattcggg caggttgtcg gacaggatct gctcttcgta ggtgaacacg
      481 cgagcgctgc gcgtggtgcc cggaatcagc cgggtggtcg agcccgccgc gatgatcaca
      541 ttgtcgaagg tgaccacctc ggtgcccgtg tcggtcttca cctcgatgct cttggcgtcg
      601 gtgaaggtgc cccacccgtt gatctcggtg atcttgttct tcttcatcag gtaatggatg
      661 ccgccgacca tgcggttcga cacctcgcgg ctgcggctga aggcccggcc gaagttgaac
      721 gtgacatcgc cttcgatgcc gaacagttcc ttgtcgtggg tgaagatgtg cgccaactcg
      781 gcgttgcgca gcagcgcctt ggtgggaatg cacccgacgt tgagacagac cccgccccac
      841 cattgctttt cgatgatggc caccttctgg ccaagctgcg cggcccggat ggcggccaca
      901 tagccgcccg gaccggcgcc caggacaacg acgtcgtaat gtgcgctcat acgatgcacc
      961 ctagtgggtc gcgtgcgcag attcagcccg tgaccgagcc gtcggtcccg atctgcggca
     1021 gtcaccgcag tgacgcggcg ttcccccttg tggacgacct gtgcgccgag cgtccggacg
     1081 gcatggctta tgctgcccgg gtgacagagc tctcccccac ccgcatcggc ttcctgatcc
     1141 gggacgcccg ccggcagcgc gggctcaccc aacaggagtt ggccgacgag ctgggcacct
     1201 cgcccggcat agtcgacgat ctcgaggccg gccatcgtgc gctgtcggtc gaactggtca
     1261 acaagatcgc cgaggcgctg gagcaaaccc agccggtcgg cggtgcactc cacctacgca
     1321 tccgcggcga gacgaagctg accgggtcga tcgccgtccg ctcgtccaag aacgcctcgg
     1381 tggccctgct gtgcgcgtgc ctgctcaacc gcgggcgcac cgtgttgcgc ggcatcgccc
     1441 agatcgaaga ggtcaaccgc attctcgagg tgctgaccag catcggcgtg cgcgccacct
     1501 ggtcgcagga ccgcaccgag ctggagctga tccggcccga ccggctgcaa ctcgaccgca
     1561 tggacgagga ggccgcccgc cgcacccgct ccatcatcat gttcctgggc ccactgctgc
     1621 acgacgaaac gaacttcgag ttgccctatg ccggcggctg caacctcggc acccgtacgg
     1681 tctcccccca ccttcacgcg ctgcgccagt tcggcctcga catcaaggcg accaagggtt
     1741 cataccatgc gcacgtcgac ccgatcgacc cctcggccta ccgatccatc accctgaccg
     1801 agcgcggcga cacagtcacc gagaacgtgc tgatggcggc cgccaggcac cccggcgaga
     1861 cggtgatccg aaacgccagc agcaactaca tggtgcagga cctgtgcttc tttcttcagg
     1921 aactcggcgt gcacatcgac ggcatcggta gcaccacgct gcgggtgcac ggccgggcgt
     1981 tgatcgacgc cgacgttgcg ttcgcaccat ccgaggatcc gatcgaggcg atgagcctgc
     2041 tcaccgcggc gatcgtcacc ggctcggagc tcaccgtgca gcgttgtccg atcgagtttc
     2101 tcgaaatcga gctgtcgatc ctgaccgaga tgggtcttcg ctacgtgctc tcgcccgagt
     2161 acccggccga gaacggacgc acccggctgg tcgacctcac ggtgcttccg tcgcagctgc
     2221 acgccaactc cgacaagatc cacccgatgc ctttccccgg gctcaacatc gacaatctgc
     2281 cgttcttcgc ggtgatcgcc gcggtcgccg agggacagac gatgatccac gactgggtgt
     2341 acgagaaccg tgcgatccac ctggtcgaac tggccaagct cggcgccaag gtgcaacttc
     2401 tcgacccgca ccgcatcatc gtcaccggcc ccacccgctg gcgcgggcag gagatcatct
     2461 ctcccccggc cctgcgtccc gcggtgtgcc tgttgctggc cgcactggca gcgcgcggca
     2521 ccagcgtgct gcgcgacgtg tacgtgatca accgcggcta cgaagacctg ccgaaccggc
     2581 tcaacgccct gggcgcccac atcgaggtct tccgcgactg agaccccggg cccaacagag
     2641 cgcgagaagc ttggcgctcg cgggccccgc tcctggtcgg accgtgaccc cgagccggca
     2701 cgactcctaa gctgacgcgg tggccattga attgttgtcc ccccgccagc tggacgagat
     2761 gcgtccggcc ggcgcgttca tcgccgaggt gttgacggct ttggtggagc acgcggccgt
     2821 gggcgtcgat ctgctcgacc tggacggtct ggcgcacgac atgatccgcg cccgcggcgc
     2881 gacctcgtgc tacatcgact acgccccctc cttcggccgt ggaccgttcg gcaaagtgct
     2941 ctgcaccagc gtcaacgacg cggtgctgca cggactgccg cacgcctacc ggctgcgcga
     3001 cggcgacctg gtcagcttcg acttcgccgc cggcgtcgac ggctgggtct gcgattcggc
     3061 ggtcagcgtg gtcgtgggca cccccgacca ggacgatctg cgactgatcg aggcgaccga
     3121 ggtggcactg gctcgcgcga tcgccatggc acgtccgggc aacaaggtcg gcgacctcag
     3181 cgccgcgatc ggcgccaccg cgcgtgaata cggttacacg gtgaacaccc agttcggcgg
     3241 gcacggtgtc ggccgcacca tgcacggcga tccccacatc tccaacgacg gacgccccgg
     3301 gcgcgggttc gcgctgcgtc ccgggctggt gatcgccatc gaaccgtggc tgatggccgg
     3361 caccgaccag atccgcatgg acgacgacgg ctggacgatc cgcagcgccg acgggtcacg
     3421 caccgcgcac agcgagcaca ccatcgcgat cacggccggt gagccgatcg tgatgaccgc
     3481 gcgcggctga ttgccggggc cgtcggtcgg gtcggatgcc cgcccgacga cgtccatggc
     3541 agtgggggta ccgtctcggg tgcccactgc cccggtgatt cccgaccggt cgccgactga
     3601 tgccggcagg cccaggttca gcgcacctgg gtgacgtcga agtggtacgt cggggtgacg
     3661 atctcatcct gcgccgccac cagctgcagc tcggacgagc ccgagtcggc ggtcagcttg
     3721 agcaccgagt agacgatgtc ggcggtgtga cccagcgcct gggccaccga accggtgcgc
     3781 agctgatgcg ccaggaacat tgccgcggtc aggtcgccgg acccggtgaa cacgcggtcg
     3841 agccgcggcg tggtgacctg ccaggctccg tcaccactga ccgcgagcag cgtcaggcaa
     3901 tcgccgtcgg tggcatcggt gaccacggag gtgaccagca cggtctcggg ccccacggct
     3961 cgcaacgcgt gcgcggcctc gatcaccgcg tccagggtgt gcgtctcccg cccggtgagg
     4021 tagtccagtt cgaaatggtt cggggtcacg atctgggcct ggggcaccac cttgtcgcgc
     4081 atgaactcgg gaataccggg ccggacgaag aagccccggc ccacgtcgcc catcaccgga
     4141 tcgcagcagt agatggcctg cgggttgcgc tgcttgacca gcgcgacggc gtcgagcacc
     4201 accgcgccga cctcttctgc accctgatag cccgacagca cggcgtccac ccggttcagc
     4261 accccgcgct catcgatgcc ctgaatcacc tctgccacgt catttgcatg cagcagcgga
     4321 ccccgccatt gcccgtaccc ggtgtgattg gagaagtgca cggtgagcac cggccagacc
     4381 tcgatgcccg agcgcatcag gggaaaggtc gcggcactgt tgcccacgtg gccgtaggcg
     4441 accgacgact ggatcgaaag gatgactgtc acgaggtcat attctggccc aggccgccgc
     4501 aacacgaaaa tggcgaccaa aaccccttgt gaagcgccaa tcggtgatca gcacagaaac
     4561 gagtccaggg ccagcgcctc ggcctcgagt tgggccgcac gctcggcctg gccagccgcc
     4621 cggtactggg cggccgcggc gagccgttcg tcgcgctcgg cactgacgat ctcgagggtc
     4681 tgttcctcgg tcagttcacg gcgctgcact tcggccgcac cgagccccac cacggcgccc
     4741 gcgatgtgct cactggtggc agcccgcgtc acgttctggt cggacggcac cgactcggcg
     4801 ttctcgatgg ccgccagggc cgctctgatg gcatttcggc caggggtgtc gcccgacttc
     4861 atggcgccgg gcagggcgtc gcgaagggcg tggcgcagcg gtgctgactc gtccggcatg
     4921 gcccgattat tgcctacgga ccgtgagatg agtaggcatg acggccattc acaccggtcg
     4981 acaccgtcac ccatactgag cccgtgcagg cccgcgacta ccgctacttc gacaccgggt
     5041 tcgccgcgct cgcccaccgc ggcggctacg cggcggacac ggatcgaacc cgagagaaca
     5101 ccctgttcgc cttcaccagt gccgccgccc tgggctaccg gtacctggag actgacgtgc
     5161 acgtgtcgag cgacggcgtg ctgttcagct ttcacgacga cacccttgac cgggtcacgg
     5221 acgcccgcgg catacccgag cggctgccct ggacggagtt gcgccaagtc agggtcggtg
     5281 gccgggaccc gatcgccacc atggacgaac tcttcgaggc cctgccccgg gcgcggttca
     5341 acatcgatct caaggtgccg ggcgcgatcg aaccgctggc ccgcaccatc gaggcgcacc
     5401 gggcgcacga ccgggtctgc gtcggatcgt tctcgcccgc cggcatcagg gccttccggc
     5461 gcctggtcgg caaccgggtg gcgaccgctg tcggcccgtg gggggtggcc tgggcaggat
     5521 acgcgcgcgg ccttcggcgg atcgtgcgct cccccggcgt ggccctgcag gtgccggcgc
     5581 ggacgtggca cgatcgggtg ccggtgctga cccgcgggct ggtgcgggcc gctcatgccg
     5641 gcggaagggt cgtccacgtg tggaccgtca acgacccagc cgagatgcac cggctgatcg
     5701 atctgggagt ggacggcctg gtcagcgacc ggatcgacgt gctgaaggcc gtgctgatcg
     5761 agcgggaccg ctgggaggag aactgatgcg cagcaccacc cggcaacgcg cgtggtattt
     5821 ctacgactgg gccaactcgg cctatgtgac caccaccgcc acggtcattc tcggccccta
     5881 cctgacgagt ctcgccaccc gggccgcctg cccggacctg cccgccggcc aggtctgcac
     5941 caacccggtg aacctcctcg gtctacccgt gctgcccggt gccgtacacc cgttgctgat
     6001 caccatctcg accatcgtga ccctggtggc gctgctgatc atcggggtgg tggccgaccg
     6061 ctccccgcac ccgcaatggt ggttgggcgg cctggcctgg gtcggtgcgg cggcggcgtc
     6121 cgcgatgttc tttctggacg gcaccaactg gcagctgggc gcggtgctga tgatcatcgc
     6181 caacctgtgc gtgggatcgt cccaggtgat ctatgacggc attctggtgc gcatcgccgc
     6241 acccgatgac cgcgatcggg tctcgagccg cggctgggcc ctgggctacc tcggcggcgg
     6301 gctgctgctg gcgttgaacc tggtgttgat gacggcctat gactcgttcg gtatcagcta
     6361 cggcctggcg atccggctca gcctgctctc tgcaggcctg tggtggggcc tcttcacgat
     6421 cattccggtg ctcggcctgc gctcgctgcc cccgcgcgcc ctggacgaac tgcagcagcg
     6481 cgccgacgtg cgccgcaacc ccgtccgaca gttggcgcac acgttcgccg accttcgcaa
     6541 gtacccgcag acgctgctgt tcctggccgc ctacctgttc tacaacgacg gcatccagac
     6601 cgtgatcggc caatccagcc tgtatgcgca atcggagctg gccatgtcga cctcacaggt
     6661 catggtcacg tttctgctca cccagttcgt ggcgttcggc ggcgccctgc tgttcggacg
     6721 ggtggcggcc cggctgggtg cgaagcgcac cgtgctgatc ggcatcggca tctggttcgc
     6781 ggtggtcgcg gtggcgttct ttctaccggc aggcgtgttc gtggcgctgc tggcgctggg
     6841 cgccgcgatc ggcctggtga tgggcggcac gcaggcgctg agccgctcga tgtactcgca
     6901 actggtgccc aagggccgcg aggccgagta cttcagcttc taccaggcga tggaacgagg
     6961 caccagttgg ctcggcacgc tcagcttcgc cctggtgttt cagttcagcc aatcctaccg
     7021 ctgggcgatc gtcgccctgg tggcattctt ccttgtcgga gggctattgc tgacccgggt
     7081 cgacatgcgc aggggaatac tccaagccgg aaacgagttg ccaaaggtgt tgtgagccgc
     7141 ttggggtcac gcggtagttt aggcaggacg gcggttgctg gcgccgctcc gtcgaaggga
     7201 gaagtcatgg cagaacgatc gttgcgcgga tcgggtttgg ggtccaagag cttcgccgac
     7261 ggtgcgggcg tcgagttcgc cgcgcgccaa gaaaccgggt tcgactgccc gaagggtcac
     7321 cacttcacgg tgaccttcgc ggaagaggcc gagttgccca ccgagtggga atgccctcgg
     7381 tgcggtggtg tggcagtccg ctcggacggt gtggtggccg aggccaaaga ggtcaagccg
     7441 ccgcgcaccc actgggacat gctgcgcgag cgtcgctcga tcgaagagct cgaagaactg
     7501 ctcgccgagc gcctggacga gattcgcggc acggactgat cgctcagcgg caccgattgt
     7561 ccctcagggg cgccgactgg tgtgtcaggg ttcgatctcg cccttgatca ccatcggacc
     7621 ggacggcccg cgctggtgat cgccggcggc cggccccggt gcgtcgggca ccgtctcgcc
     7681 acggatcacc gtgtcgcgct ctaccttggc ccgcatcaca tcgacgttga cgcccgctcg
     7741 cgccgcccgg cgcgagatga acagctgcag caggttgcgc gcgaacggcc gggtgaacgg
     7801 cagcaggaag atcagcccga aaatgtcggt gaaga
//
LOCUS       DAOSUG010000067         7810 bp    DNA     linear   ENV 10-JUN-2024
DEFINITION  MAG TPA_asm: Micropruina sp. isolate AMR_MDS_2195
            NODE_4720_length_7810_cov_3.833914, whole genome shotgun sequence.
ACCESSION   DAOSUG010000067 DAOSUG010000000
VERSION     DAOSUG010000067.1
DBLINK      BioProject: PRJNA925477
            BioSample: SAMN32864262
            Sequence Read Archive: SRR4236673
KEYWORDS    WGS; ENV; Metagenome Assembled Genome; MAG; Third Party Data; TPA;
            TPA:assembly.
SOURCE      Micropruina sp. (wastewater metagenome)
  ORGANISM  Micropruina sp.
            Bacteria; Bacillati; Actinomycetota; Actinomycetes;
            Propionibacteriales; Nocardioidaceae; Micropruina.
REFERENCE   1  (bases 1 to 7810)
  AUTHORS   Abdulkadir,N., Saraiva,J.P., Zhang,J., Stolte,S., Gillor,O.,
            Harms,H. and Rocha,U.
  TITLE     Genome-centric analyses of 165 metagenomes show that mobile genetic
            elements are crucial for the transmission of antimicrobial
            resistance genes to pathogens in activated sludge and wastewater
  JOURNAL   Microbiol Spectr 12 (3), e0291823 (2024)
   PUBMED   38289113
REFERENCE   2  (bases 1 to 7810)
  AUTHORS   Abdulkadir,N.
  TITLE     Direct Submission
  JOURNAL   Submitted (24-MAR-2023) Environmental Microbiology, Helmholtz
            Center for Environmental Research -UFZ, Permoserstr 15, Leipzig
            04318, Germany
COMMENT     The annotation was added by the NCBI Prokaryotic Genome Annotation
            Pipeline (PGAP). Information about PGAP can be found here:
            https://www.ncbi.nlm.nih.gov/genome/annotation_prok/
            
            ##Genome-Assembly-Data-START##
            Assembly Method        :: SPAdes v. 3.11.1
            Genome Representation  :: Full
            Expected Final Version :: Yes
            Genome Coverage        :: Not Applicable
            Sequencing Technology  :: Illumina MiSeq
            ##Genome-Assembly-Data-END##
            
            ##Genome-Annotation-Data-START##
            Annotation Provider               :: NCBI
            Annotation Date                   :: 05/04/2023 04:57:10
            Annotation Pipeline               :: NCBI Prokaryotic Genome
                                                 Annotation Pipeline (PGAP)
            Annotation Method                 :: Best-placed reference protein
                                                 set; GeneMarkS-2+
            Annotation Software revision      :: 6.5
            Features Annotated                :: Gene; CDS; rRNA; tRNA; ncRNA
            Genes (total)                     :: 2,776
            CDSs (total)                      :: 2,750
            Genes (coding)                    :: 2,714
            CDSs (with protein)               :: 2,714
            Genes (RNA)                       :: 26
            tRNAs                             :: 24
            ncRNAs                            :: 2
            Pseudo Genes (total)              :: 36
            CDSs (without protein)            :: 36
            Pseudo Genes (ambiguous residues) :: 0 of 36
            Pseudo Genes (frameshifted)       :: 2 of 36
            Pseudo Genes (incomplete)         :: 32 of 36
            Pseudo Genes (internal stop)      :: 2 of 36
            ##Genome-Annotation-Data-END##
FEATURES             Location/Qualifiers
     source          1..7810
                     /organism="Micropruina sp."
                     /mol_type="genomic DNA"
                     /submitter_seqid="NODE_4720_length_7810_cov_3.833914"
                     /isolate="AMR_MDS_2195"
                     /isolation_source="Activated sludge of municipal
                     wastewater treatment plants"
                     /db_xref="taxon:2737536"
                     /environmental_sample
                     /geo_loc_name="Canada"
                     /lat_lon="49.25 N 123.1 W"
                     /collection_date="2015-06-16"
                     /metagenome_source="wastewater metagenome"
                     /note="metagenomic"
     gene            complement(78..761)
                     /locus_tag="PLF56_03535"
     CDS             complement(78..761)
                     /locus_tag="PLF56_03535"
                     /EC_number="3.2.2.27"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_002514142.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_function: GO:0004844 - uracil DNA N-glycosylase
                     activity [Evidence IEA];
                     GO_process: GO:0006284 - base-excision repair [Evidence
                     IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="uracil-DNA glycosylase"
                     /protein_id="HOQ52667.1"
                     /translation="MNPKPLPELIAADWAQALAPVADQIAAMGEFLRTELAAGRGYLP
                     AGANVLRAFTRPLAEVRVLIVGQDPYPTPGHAVGLSFSVAPQVRPLPRSLVNIYAELS
                     TDLGIAPAAHGDLTGWFEQGVLLLNRVLTVRPGAPGSHRGKGWEAVTQQAIEALVARG
                     GPLVAILWGRDAQNLTPRLGDVPVIASAHPSPLSARSGFFGSRPFSRTNAALEAQGAA
                     PIEWTLPAA"
     gene            complement(758..1954)
                     /locus_tag="PLF56_03540"
     CDS             complement(758..1954)
                     /locus_tag="PLF56_03540"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_013865256.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_function: GO:0022857 - transmembrane transporter
                     activity [Evidence IEA];
                     GO_process: GO:0055085 - transmembrane transport [Evidence
                     IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="MFS transporter"
                     /protein_id="HOQ52668.1"
                     /translation="MRLSLRTTLPRTVVMLAVCAFFVAVGFGVMIPVLPLFAASFGVT
                     NFLIGLVVSAFAGMRLAMSPFVRRISDRIGHRRTIGVGMWIVGGSSALCGFAASYGEL
                     LFWRAVGGLGSAMFTISATALLLASVGPELRGRASGLYQGGFLLGGMTGPAIGGLLAT
                     ISLTAPFFFYAGTLVIAGIIAFTQLHDPPGMAEAAAERLTRPLRTVLRDVRFQAACLL
                     GFGTGWQSFGVRSALVPILIVSYLGHPPSWTGIAFAIAAVAQTLALQPAGRATDLIGR
                     RPVMVTGGVLAAASTLATPFAPNLTVLIVLLCVYGVASAALGTAPTAALGDAAKGAGS
                     QPIAIFSMVGDSGAILGPLAAGLLADHLGMGAAFASGAVILLAGALWAWLIPARLDRR
                     APQEAR"
     gene            complement(2182..3072)
                     /locus_tag="PLF56_03545"
     CDS             complement(2182..3072)
                     /locus_tag="PLF56_03545"
                     /inference="COORDINATES: protein motif:HMM:NF021604.2"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="anti-sigma factor"
                     /protein_id="HOQ52669.1"
                     /translation="MTDDAHDQIGAYAVDALSADERATFEQHLTTCAECQTELRGLRE
                     SLAGLAPEYETEPPATLRAAVLAAVAQTPAPLADPNPALVAEPSVTSDTPDGAIQPGP
                     RQEPSSNGHRGHEGERAAPDGVAPGRLVSPADGSLASVTPLPPRPSAGAGSGRTRWQL
                     LVAAAVALVAVIGIAVWQPWVARTVTAADVLNSPDAARATTSDQGMSVTLVRSNQLGR
                     AVLIAQNLPAAPSGKVRQAWLQHPGEHMVSGGLMPSGADVTMLLEGDARDATGAGISI
                     EPSGGSTQPTIKDVVALVQF"
     gene            complement(3075..3653)
                     /gene="sigK"
                     /locus_tag="PLF56_03550"
     CDS             complement(3075..3653)
                     /gene="sigK"
                     /locus_tag="PLF56_03550"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_020385205.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_function: GO:0003700 - DNA-binding transcription factor
                     activity [Evidence IEA];
                     GO_function: GO:0016987 - sigma factor activity [Evidence
                     IEA];
                     GO_process: GO:0006352 - DNA-templated transcription
                     initiation [Evidence IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="ECF RNA polymerase sigma factor SigK"
                     /protein_id="HOQ52670.1"
                     /translation="MEEVDPVVTTTRADPVADALTGCASGDQAAMAILYDLTSAKVYG
                     LSLRVLRNPAQAEEVAQEIYLEAWQNSTTFDAARGTGLAWLLTITHRRAVDRVRSAQA
                     STRRDTTYGLEDLATAPEDPADTAIASLEAQRVRGAMTGLTDLQREAVELAYFGGRTH
                     REVADALDVPLGTVKTRIRDGLTRLRSALGGD"
     gene            complement(3773..4408)
                     /locus_tag="PLF56_03555"
     CDS             complement(3773..4408)
                     /locus_tag="PLF56_03555"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_017935583.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="fasciclin domain-containing protein"
                     /protein_id="HOQ52671.1"
                     /translation="MRTSIKLLGASAALLMPLAACSSTTSAMPSTTAPMASTSAPMTS
                     ASSMPTPSESTMAQFGPACASVPSSGAGSFEGMAKDPVATAASNNPALSTLVTAVKKA
                     GLVDTLNNAKDITVFAPTNDAFAKLPKKDLEAALNDKATLTKILTNHVVAGRLAPDQL
                     AGKHDTLAKTSLTVTGSGEDFMVGDAKVVCGNVQTSNATVYIIDSVLLPKM"
     gene            complement(4679..6250)
                     /locus_tag="PLF56_03560"
     CDS             complement(4679..6250)
                     /locus_tag="PLF56_03560"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_008362507.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="molybdopterin-dependent oxidoreductase"
                     /protein_id="HOQ52672.1"
                     /translation="MPDDSLTRTPSVDAPRSPAPRGRAGRAQAPAAGALAVLAGLAAG
                     HLIAALTGPDSSPALAVGSRLIDLTPTPVKEWAVATLGTWDKPVLIGGVVLATLAIGA
                     LVGWLASRRRLLIWVGPLVLTVIAAVAASTGPAAGPLAMVPAVVTGGVGTWVLWWFLG
                     RTTIRPDEPGAPYALPRRAFLSGSAVVGASSAVALVAGQYLGTARQRPAVVTLPRPTE
                     TLAPLPSGLSVDGLAPWVTPNRDFYRVDTSLIVPSVDVGEWQLSIDGMVDRPFTLTWS
                     ELLALPMIERDVTLNCVSNEVGGPYIGGARWLGVRVRDVLARAGVQAGADQVLSRSVD
                     GFTVSTPVQALTDARDALIAIGMNGSPLPAEHGFPARLITPGLYGYVGATKWLTQLTL
                     TTYARQSAYWTERGWAERGPVKPSSRIDTPRSFARLQPGQVMIAGEAWAQNIGVLRAQ
                     VRIDGGPWHDAELGPDAGIQYWRQWKYAWAATSGQHNLECRVVDESGANQSEARATPF
                     PDGSSGLQSVVVTVD"
     gene            6356..7522
                     /locus_tag="PLF56_03565"
     CDS             6356..7522
                     /locus_tag="PLF56_03565"
                     /inference="COORDINATES: protein motif:HMM:NF013740.2"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_function: GO:0015562 - efflux transmembrane transporter
                     activity [Evidence IEA];
                     GO_process: GO:1905887 - autoinducer AI-2 transmembrane
                     transport [Evidence IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="AI-2E family transporter"
                     /protein_id="HOQ52673.1"
                     /translation="MPFSLFRRSRRRAALPATATDSPVVAVAIAEPEAENPIPQGVRT
                     AAGWAWRLLLFAALVWGAGWLLGEFSGVSVPLAIAFLLTALLSPLAHRLKTWNWHPLL
                     AAAACLLAMLLILAGIGWGIGATAAKQAPELVQQSVAGFNQLMQWVADGPLGIDQGQI
                     DQWMQSLTSWLTESRAVLAGYAARVGASVGHFFAGVAIALIATFFFLAEGQHLWRSSV
                     GLLPQRYQAATGQAAERGWASLVSYMRAQVIVSAVDAAGVATIAAIIGVPMALALFAL
                     TFIVCFIPVVGAIIAGTVATSLALITHGWVAALIMAGGTVAVMWLESHLLQPLLLGKA
                     ASLHPLAVLIGIAVGAQVGGIVGALVVIPTMAFFVAFIRSLRGKPVQETTHKRK"
     gene            7571..>7810
                     /locus_tag="PLF56_03570"
     CDS             7571..>7810
                     /locus_tag="PLF56_03570"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_002527866.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="hypothetical protein"
                     /protein_id="HOQ52674.1"
                     /translation="MATEPDLTPILEDLAAGRIDASEAARRIDALKAAAEQTSEPSDD
                     EIAGDTDSPGQHASGVFNQPPFVEDVPPSPRRATGA"
BASE COUNT         1173 a         2719 c         2736 g         1182 t
ORIGIN      
        1 aggatgacaa cgcagcgagc gggcgtactg ggtgcgccgc agcgcggtca gggttttacg
       61 acacgcccta ggcggcccta ggcggcgggt agtgtccact cgatcggggc ggcgccctga
      121 gcctcgaggg ccgcgttcgt ccggctgaac gggcggctgc cgaagaatcc cgatcgtgcc
      181 gacagcgggg acggatgggc cgacgcgatc accggcacgt caccaagcct cggggtgagg
      241 ttctgcgcat cccggcccca cagaatcgcg accagtggcc cgccgcgtgc gaccagcgcc
      301 tcgatcgcct gttgggtgac cgcctcccaa cccttgccgc gatgcgatcc gggggcgccc
      361 ggacgcaccg tcagcacccg gttgagcagc agcaccccct gctcgaacca gccggtcagg
      421 tcgccatgcg cagccggggc gatgcccaga tcggtgctca gttcggcgta gatgttgacc
      481 aacgatcgcg gcagcggacg cacctgcggc gccaccgaga acgacaaccc gaccgcatga
      541 cccggcgtgg ggtaggggtc ctgcccgacg atcagcaccc gcacctcggc caacggacgc
      601 gtgaacgccc gaagcacgtt cgcaccggcc ggaaggtaac cccgtccggc ggccagttcg
      661 gtgcgcagga actcgcccat cgccgcgatc tggtcggcga cgggcgccag ggcctgtgcc
      721 cagtccgctg cgatcagctc gggcaggggc ttggggttca tcgtgcctcc tggggtgcgc
      781 ggcgatcgag tcgagcgggg atcaaccagg cccacagtgc gcccgcgagc agaatgaccg
      841 cacccgaggc gaacgccgct cccatgccga ggtgatcggc cagcaacccg gcggccagcg
      901 gccccaggat cgcgccgctg tcgccgacca tgctgaagat cgcgatgggc tggctaccgg
      961 cgcccttcgc tgcgtcgccc agtgccgcgg tcggtgccgt gccgagggcg gccgacgcca
     1021 cgccgtagac gcacagcagc acgatgagca cggtcaggtt cggcgcgaac ggcgtggcca
     1081 gcgtggatgc tgccgccagc accccgccgg tgaccatcac cggacgacgt ccgatcaggt
     1141 cggtcgcacg gccggccggt tgaagtgcca gcgtctgggc gaccgccgcg atggcgaagg
     1201 cgatgcccgt ccaggacggg gggtggccga ggtagctgac gatcaggatc gggaccagcg
     1261 ccgaacgcac cccgaacgac tgccaaccgg taccgaagcc gagcaggcag gcggcctgaa
     1321 agcggacgtc gcgcagcacg gtccgcagcg gtcgagtaag tcgctcggcg gccgcctcgg
     1381 ccatgcccgg ggggtcgtgc aactgggtga aggcgatgat gccggcgatc accagggtgc
     1441 cggcgtagaa gaagaacggt gccgtcagcg agatcgtggc gagcaggccg ccgatcgccg
     1501 gacccgtcat gccgccgagc agaaaaccgc cctggtagag gccgctggca cgcccgcgca
     1561 gttccggacc cacgctcgcc aacagcaggg cggtggccga gatggtgaac atggccgaac
     1621 ccaggcctcc gacggcacgc cagaagagca gttcgccgta gcttgcggcg aaaccgcaca
     1681 gcgcggacga gccgccgacg atccacatgc cgacaccgat ggtgcgccga tgcccgatcc
     1741 ggtcgctgat ccgacgtacg aaggggctca tcgccagccg catgcccgcg aacgccgaca
     1801 ccaccagccc gatcagaaag ttggtcacgc cgaatgaggc tgcgaacagc ggcagtacgg
     1861 gaatcatcac cccgaagccg accgcgacga agaaggcgca gaccgccagc atcaccacgg
     1921 tgcggggcag cgtggtgcgc agcgacaatc tcaccgggcc aacctagtgc cccggccacc
     1981 aacctttgct cgctgcgcga cgctcaggcc tgaaaagcct gcaccagtgg cagacctcgc
     2041 ggcgcgcgca ggcacgaccc cctgcactgg tgcagggatt tcaggcggtg ccctcggcga
     2101 ccgtgccgaa ggatgcaccg tccgacgcga ctgcagggat tccaggcgcc gtgggtggcg
     2161 gctggttcgg cgccgtcggg gtcagaactg caccagcgcc acgacgtctt tgatcgtggg
     2221 ctgcgtcgag ccgccggagg gttcgatgct gatgcccgca ccggtggcgt cgcgcgcgtc
     2281 accttccagc aacatcgtca cgtcggcgcc ggacggcatc aggccacccg aaaccatgtg
     2341 ctcgcccgga tgctgcagcc aggcctgcct gacctttccg ctgggcgcag caggcaggtt
     2401 ctgtgcgatc agcacggccc gaccgagctg gttggaccgc acgagagtga cgctcatccc
     2461 ctggtcggag gtggtggcgc gggcggcgtc cggagagttc agcacgtccg ccgcggtgac
     2521 cgtgcgggcg acccagggtt gccacacggc gatgccgatc accgccacca gggccaccgc
     2581 cgctgcgacc agaagctgcc agcgggtgcg tcccgagccg gcaccagcgg acgggcgggg
     2641 cggcagcggc gtcaccgaag cgagggaacc gtcggccggc gaaacaaggc gtcctggcgc
     2701 cactccgtcc ggcgccgccc gttcaccctc atgaccccgg tgcccattgc tggacggctc
     2761 ctgccggggc ccgggctgaa tcgcaccgtc cggagtgtcc gaggtcaccg acggctctgc
     2821 caccagtgcg ggattcgggt cggccagcgg ggcaggggtc tgtgcgacgg cggccagcac
     2881 agccgcgcga agggtggcgg gcggctcggt ctcgtactcg ggggcgagcc cggccaggct
     2941 ctcacgcagg ccgcgcagtt cggtctgaca ctcagcacag gtggtgaggt gttgctcgaa
     3001 cgtggcgcgc tcgtcggcgc tcagggcgtc caccgcgtag gcgccgatct ggtcgtgtgc
     3061 atcgtcagtc acgatcagtc accccccaat gctgatctca ggcgggtcaa tccgtccctg
     3121 attcgagtct tcaccgtgcc cagcggcacg tccagcgcgt ccgccacctc gcggtgggtg
     3181 cggccgccga agtaggcgag ttcgaccgcc tcgcgctgca ggtcggtcag cccggtcatg
     3241 gcgccgcgca cccgctgcgc ctccagcgag gcgatggccg tgtcggcggg gtcctccggt
     3301 gcggtggcga gatcctcgag gccgtaggtg gtgtcgcggc gggtcgaggc ctgggccgag
     3361 cgcacccggt cgacggcgcg ccggtgggtg atggtcaaca accaggcgag gccggtgccc
     3421 cgcgcggcgt cgaaggtggt gctgttctgc cacgcttcga ggtagatctc ttgcgcgacc
     3481 tcttcggcct gcgccgggtt gcgcagcacc cgcagtgaca agccgtacac cttggccgag
     3541 gtgaggtcgt acaggatcgc catcgctgcc tggtcgccgc tcgcgcaccc ggtcagggcg
     3601 tcggcaaccg gatcggcacg ggtagtcgtg actaccggat ccacctcctc cacagcgatc
     3661 agtatgggtg ctggtgtgcg gaaatggatg gatttgggcg tggcgtccca cctgatcgag
     3721 tgtgacgcca cgccccctcc ccccggacgt ggcctgcagg ccacgggtca ggtcacatct
     3781 tcggcagcaa cacgctgtcg atgatgtaga ccgtggcatt cgaggtctgg acgttgccgc
     3841 agaccacctt ggcgtcgccg accatgaagt cctcaccgga gccggtgacg gtcagcgagg
     3901 tcttggccag cgtgtcgtgc ttgccggcga gctgatcggg cgccagccgt ccggccacca
     3961 cgtggttggt cagaatcttg gtcagcgtgg ccttgtcgtt gagcgcggcc tcgaggtcct
     4021 tcttcggcag cttcgcgaag gcgtcgttgg tgggtgcgaa cacggtgatg tccttggcgt
     4081 tgttgagcgt gtcgaccagg cccgccttct tcacggcggt gaccagggtc gacagggccg
     4141 ggttgttgct ggcggccgtg gcgaccggat ccttcgccat gccctcgaac gagccggcgc
     4201 cactggacgg cacgctcgcg caggccgggc cgaactgcgc catcgtcgat tcggacgggg
     4261 tgggcatcga agacgccgag gtcatggggg cggacgtcga ggccatcggc gccgtcgtgc
     4321 tgggcatcgc cgaggtggtg ctgctgcagg ccgccaacgg catcagcagg gcggcggagg
     4381 ctccgagaag cttgatcgag gtgcgcatgg tggtgtctcc ttctgccggg gatccggcgg
     4441 tggtgtacct ctctgacacc aggtgttcgg aacggcgccg ggtgcggatt ggtggtgtgg
     4501 ggactgtttg tcagcacggt tcagaagcat tcccagcgcg gtcagggaag gttctcaaca
     4561 cggttcttga ctgttctcaa cacggttcgg gacggttcgg ggacggtctc agcaccgccg
     4621 agtaccgttc ccggttcgct tcaacaggtt ctgcgcgtct tctttgccaa gatcctgctc
     4681 agtcgaccgt gaccacgacg ctctgcagcc cggacgatcc gtccgggaaa ggcgtggcgc
     4741 gcgcctcgga ttggttcgca cccgattcgt ccaccacccg acactccagg ttgtgctggc
     4801 cggacgtggc ggcccaggcg tatttccact gccgccagta ctgaatgccg gcgtccggcc
     4861 cgagttcggc gtcgtgccac gggccaccgt cgatgcgcac ctgcgccctc agcacgccga
     4921 tgttctgcgc ccacgcctcg ccagcgatca tcacctgacc gggctgcaac cgcgcgaacg
     4981 accgcggcgt gtcgatccgg ctggagggct tcaccggccc ccgctcggcc cagccccgct
     5041 ccgtccagta cgcgctctgg cgggcgtacg tcgtcagcgt cagttgcgtc agccacttcg
     5101 ttgcgcccac gtagccgtac agccccggcg tgatcagcct ggccggaaac ccgtgctcgg
     5161 cgggcagggg actgccgttc atgccgatcg cgatcagtgc gtcccgcgcg tcggtcagcg
     5221 cctggacggg tgtgctgacc gtgaagccgt ccaccgaccg gctgagcacc tggtcggccc
     5281 cggcctgcac gccggccctg gccagcacgt cacgcacccg cacgcccagc cagcgtgcgc
     5341 cgccgatgta gggcccgccg acctcgttgc tgacgcagtt gagggtcacg tcgcgttcga
     5401 tcatcggcag ggccagcagt tcgctccagg tcagggtgaa cgggcggtcc accatgccgt
     5461 cgatgctcaa ctgccactcg cccacgtcga cgctgggcac gatcaggctg gtgtcgaccc
     5521 ggtagaagtc gcggttgggc gtgacccagg gtgcgaggcc gtccacgctc agcccggacg
     5581 gcagcggcgc cagtgtctcg gtcggccgcg gcagcgtgac gaccgccgga cgctgccgcg
     5641 ccgtgcccag gtactgcccc gccaccagcg cgacggcgct cgaggcgccc accaccgcgc
     5701 tcccgctcaa gaacgcacgt cgcggcaggg cgtacggcgc gccgggttcg tccggacgga
     5761 tggtggtacg gcccaggaac caccacagca cccaggtgcc gacgccgccg gtgaccactg
     5821 ccggtaccat cgccaacggt ccggctgccg gcccggtgga cgccgccacc gccgcgatca
     5881 cggtgagcac cagcggcccg acccagatca gcaaccgacg gcgcgaggcc agccagccga
     5941 ccagggcgcc gatcgccaac gtggccagca cgacccctcc gatcagcacc ggcttgtccc
     6001 aggtgccgag ggtggcgacc gcccactcct tcaccggtgt cggcgtcaga tcgatcagtc
     6061 ggctgcccac agccagcgcg ggcgacgagt ccgggccggt cagcgcggca atcagatggc
     6121 ccgcggccaa accggccagc acggccaggg cccccgcggc cggggcctgc gctcgaccgg
     6181 cgcgaccgcg cggcgccggg cttcgggggg cgtcgacgct cggggttcga gtgaggctgt
     6241 cgtcgggcat ggcgcctcct ttcctcaccc ggctgttcgg tgcggacggc acctcggatg
     6301 ggtctgtcag agcctcacag tagggtgggc gcgaccccca cgaaaggacg agcgcgtgcc
     6361 cttcagcttg tttcgccgat cccgccggcg cgccgcactg cctgcgaccg ccacggattc
     6421 acccgtggtg gccgtcgcga ttgccgaacc cgaggcagag aatccgatcc cccagggcgt
     6481 ccgcaccgcc gccggttggg cctggcggct gttgctgttc gcggccctgg tgtggggggc
     6541 ggggtggctg ctcggcgagt tctcgggcgt cagcgtgccc ctggccatcg cgtttctgct
     6601 caccgcgctg ctcagcccgc tcgcgcatcg gctgaagacc tggaactggc atccgctgct
     6661 ggcggctgcc gcctgcctgt tggccatgtt gttgatcctc gccggcatcg gttggggcat
     6721 cggggcgacg gccgccaagc aggcgcctga gctggttcag cagtcggtgg ccgggttcaa
     6781 ccaactgatg cagtgggtgg ccgacgggcc gctcggcatc gatcagggcc agatcgatca
     6841 gtggatgcag tcgctcacca gctggctcac cgaatcgcga gccgtgctgg ccgggtacgc
     6901 agccagggtg ggcgcgtccg ttggccactt cttcgcgggc gtggcgatcg ccctgatcgc
     6961 gacgttcttc ttcctggccg agggccagca cctgtggcgc tcgagtgtcg gcttgttgcc
     7021 gcagcgctat caggccgcca cgggccaggc cgccgaacgc ggttgggcct ccctggtcag
     7081 ctacatgcgg gcgcaggtca tcgtctcggc cgtggacgcc gccggcgtgg ccaccatcgc
     7141 cgccatcatc ggggtaccga tggcgctggc gttgttcgcc ctgaccttca tcgtctgctt
     7201 cattccggtg gtgggcgcga tcatcgccgg caccgtggcc acgtcgctcg ccctgatcac
     7261 ccacggctgg gtggccgcac tgatcatggc cgggggcacg gtcgccgtga tgtggctcga
     7321 gtcgcacctg ctgcagccgc tgctgctggg caaggcggct tcgctgcacc cgctggccgt
     7381 gctgatcggc atcgccgtcg gcgcccaggt gggcggcatc gtcggtgcgc tggtggttat
     7441 tccgaccatg gcgttcttcg tggccttcat tcgttcactg cgtggcaaac ccgtgcaaga
     7501 gaccacgcac aaacgaaaat aatgaccccc aagtctttcg attctgtaaa acagcgttta
     7561 catttgatgc atggctactg aaccggacct cactccgatc ctcgaagacc ttgccgcggg
     7621 gcgcatcgac gcctccgagg ccgccaggcg aatcgacgcg ctcaaggcag cggccgagca
     7681 gacgagcgaa cccagcgacg acgaaatcgc gggcgacacc gacagcccgg ggcagcacgc
     7741 gtccggggtg ttcaatcagc cgcccttcgt cgaggacgtg ccgcccagcc cgcggcgtgc
     7801 caccggcgcg
//
LOCUS       DAOSUG010000068         7809 bp    DNA     linear   ENV 10-JUN-2024
DEFINITION  MAG TPA_asm: Micropruina sp. isolate AMR_MDS_2195
            NODE_4721_length_7809_cov_4.854527, whole genome shotgun sequence.
ACCESSION   DAOSUG010000068 DAOSUG010000000
VERSION     DAOSUG010000068.1
DBLINK      BioProject: PRJNA925477
            BioSample: SAMN32864262
            Sequence Read Archive: SRR4236673
KEYWORDS    WGS; ENV; Metagenome Assembled Genome; MAG; Third Party Data; TPA;
            TPA:assembly.
SOURCE      Micropruina sp. (wastewater metagenome)
  ORGANISM  Micropruina sp.
            Bacteria; Bacillati; Actinomycetota; Actinomycetes;
            Propionibacteriales; Nocardioidaceae; Micropruina.
REFERENCE   1  (bases 1 to 7809)
  AUTHORS   Abdulkadir,N., Saraiva,J.P., Zhang,J., Stolte,S., Gillor,O.,
            Harms,H. and Rocha,U.
  TITLE     Genome-centric analyses of 165 metagenomes show that mobile genetic
            elements are crucial for the transmission of antimicrobial
            resistance genes to pathogens in activated sludge and wastewater
  JOURNAL   Microbiol Spectr 12 (3), e0291823 (2024)
   PUBMED   38289113
REFERENCE   2  (bases 1 to 7809)
  AUTHORS   Abdulkadir,N.
  TITLE     Direct Submission
  JOURNAL   Submitted (24-MAR-2023) Environmental Microbiology, Helmholtz
            Center for Environmental Research -UFZ, Permoserstr 15, Leipzig
            04318, Germany
COMMENT     The annotation was added by the NCBI Prokaryotic Genome Annotation
            Pipeline (PGAP). Information about PGAP can be found here:
            https://www.ncbi.nlm.nih.gov/genome/annotation_prok/
            
            ##Genome-Assembly-Data-START##
            Assembly Method        :: SPAdes v. 3.11.1
            Genome Representation  :: Full
            Expected Final Version :: Yes
            Genome Coverage        :: Not Applicable
            Sequencing Technology  :: Illumina MiSeq
            ##Genome-Assembly-Data-END##
            
            ##Genome-Annotation-Data-START##
            Annotation Provider               :: NCBI
            Annotation Date                   :: 05/04/2023 04:57:10
            Annotation Pipeline               :: NCBI Prokaryotic Genome
                                                 Annotation Pipeline (PGAP)
            Annotation Method                 :: Best-placed reference protein
                                                 set; GeneMarkS-2+
            Annotation Software revision      :: 6.5
            Features Annotated                :: Gene; CDS; rRNA; tRNA; ncRNA
            Genes (total)                     :: 2,776
            CDSs (total)                      :: 2,750
            Genes (coding)                    :: 2,714
            CDSs (with protein)               :: 2,714
            Genes (RNA)                       :: 26
            tRNAs                             :: 24
            ncRNAs                            :: 2
            Pseudo Genes (total)              :: 36
            CDSs (without protein)            :: 36
            Pseudo Genes (ambiguous residues) :: 0 of 36
            Pseudo Genes (frameshifted)       :: 2 of 36
            Pseudo Genes (incomplete)         :: 32 of 36
            Pseudo Genes (internal stop)      :: 2 of 36
            ##Genome-Annotation-Data-END##
FEATURES             Location/Qualifiers
     source          1..7809
                     /organism="Micropruina sp."
                     /mol_type="genomic DNA"
                     /submitter_seqid="NODE_4721_length_7809_cov_4.854527"
                     /isolate="AMR_MDS_2195"
                     /isolation_source="Activated sludge of municipal
                     wastewater treatment plants"
                     /db_xref="taxon:2737536"
                     /environmental_sample
                     /geo_loc_name="Canada"
                     /lat_lon="49.25 N 123.1 W"
                     /collection_date="2015-06-16"
                     /metagenome_source="wastewater metagenome"
                     /note="metagenomic"
     gene            complement(<1..302)
                     /locus_tag="PLF56_03575"
     CDS             complement(<1..302)
                     /locus_tag="PLF56_03575"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_008355276.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_component: GO:0016020 - membrane [Evidence IEA];
                     GO_function: GO:0008324 - monoatomic cation transmembrane
                     transporter activity [Evidence IEA];
                     GO_process: GO:0006812 - monoatomic cation transport
                     [Evidence IEA];
                     GO_process: GO:0055085 - transmembrane transport [Evidence
                     IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="cation transporter"
                     /protein_id="HOQ52675.1"
                     /translation="MAGFFVVELLAAWNSGSLALLSDAGHVAADVTTLGAALIATRLA
                     TRKDTTGLHTFGSYRAEVFASGLAVLLMIGVGVYIAIEAVGRIGAAPELASGLMLVV"
     gene            384..1211
                     /locus_tag="PLF56_03580"
     CDS             384..1211
                     /locus_tag="PLF56_03580"
                     /inference="COORDINATES: ab initio
                     prediction:GeneMarkS-2+"
                     /note="Derived by automated computational analysis using
                     gene prediction method: GeneMarkS-2+."
                     /codon_start=1
                     /transl_table=11
                     /product="hypothetical protein"
                     /protein_id="HOQ52676.1"
                     /translation="MSVDYLRHYFGSEAGVESLASASPGSAGRNRTLSIKNFEQVLVP
                     VPSRPDQDRIVAHLGRHTRPGRDDSTLDALVAGFTARGIEESPLVPFGSLLQLQREPV
                     ELVDGERYRRIGIYSWGKGMLRRESASANEMGSMRYFTFPIPSLVFSNNQAWEGAVAL
                     ASPEDGGHVCSSRFYPYVPREGVDVDLRFLLEFFRSDQGIALMRRASPGTQVRNKLLS
                     RSALEMAVVPFPGRTIQDRIVTRRQMVDKATDVIKRADELADAILPAARNEIFNAMR"
     gene            complement(1195..1878)
                     /locus_tag="PLF56_03585"
     CDS             complement(1195..1878)
                     /locus_tag="PLF56_03585"
                     /inference="COORDINATES: protein motif:HMM:NF015935.2"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="JAB domain-containing protein"
                     /protein_id="HOQ52677.1"
                     /translation="MTSPPLLTWPAHDRPRERLPRERLPREGSHALSDAELVAILLGT
                     GGRGRNAVALAQAVLARCGGVAGLARWGVPELPRLDALGPAKASRLVAALALAGRLGS
                     PGEPPRLADSGTIAEVARARLAGARVERLVVLVADSGLRLRHLEDVAKGGASGCGLPV
                     REVLSAVSCHDGLAIALAHNHPGGDPTPTDADRAATGQVADGGHVGLRLLDHVVVADV
                     AWRSVTASR"
     gene            2081..4606
                     /locus_tag="PLF56_03590"
     CDS             2081..4606
                     /locus_tag="PLF56_03590"
                     /inference="COORDINATES: protein motif:HMM:NF015210.2"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="DUF4268 domain-containing protein"
                     /protein_id="HOQ52678.1"
                     /translation="MVKANETNLEGILEGKRQYQVPLYQRVYSWGPKQIDQLWDDLIE
                     IAEARRAGGTPTHFIGSLVLAASPDNGVVGVHKFLVVDGQQRLTTLSILLAAIRDHLS
                     ESDGQEHRDRIDAQYLVNKYEAGKPPKLVPTQADRSAYLAIVRASAEAGGEDAVGAAY
                     RRFRVKLAEIDDPEDPHDIAAIENAVLRGLALVAVTAEAGDNAHRIFESLNNTGLRLT
                     QSDLLKNHLFMRLGDRAEEVYEALWRPLEDKLGAENLELLFWLDLVHNDEKAKQSDTY
                     LGQQRRFDTLTTAAEVAAEIRRIAKLGDVLATILKPEREQNQALRKRLERIRAWGSTT
                     AYPIVMQVLAQRAAGRASDDKAIAALLCLESYFVRRIVIGRATAGLNRTLLQAVGAIT
                     EASDVEAALRDYLSEGRKHFATDAQVREAVTTVPFYWQGRASQKKLILLWLEEALRPK
                     EPVDAKQLTIEHVLPQTLTDAVRAEFAASLPAEAEVAFEHERLVHTLGNLTLSGYNSE
                     LSNRPFSQKRTMLAESGVRMNQTIAEHQTWGASEITERAQALAERIVELWPGPNEKLV
                     GIRDHESELRLDVAAVLAGIPSGRWTTYGEVAVVVGSHPVPVGSAIANHPMPNPWRVL
                     QSGGTVSDQFRWTDRGRTDDPRDLLRDEGVDFEEAGRADPERFMDAVELASGVGMDID
                     ASTLRRRRRRQARKTTTDPGALGVQQVAFWGSVQDWGREHATHASFHRSPGRRHWYSV
                     EPAGLALQVNLNVNTMKPRVYVEIYLEGGKEQFHALHNQRKEIEGDLGYAVEWREQPE
                     KKASRIIRERPGDFRDPAQADQLVQWLVRTADDFVRVLQRLGG"
     gene            complement(5112..5927)
                     /locus_tag="PLF56_03595"
     CDS             complement(5112..5927)
                     /locus_tag="PLF56_03595"
                     /inference="COORDINATES: ab initio
                     prediction:GeneMarkS-2+"
                     /note="Derived by automated computational analysis using
                     gene prediction method: GeneMarkS-2+."
                     /codon_start=1
                     /transl_table=11
                     /product="hypothetical protein"
                     /protein_id="HOQ52679.1"
                     /translation="MDAVAPEYVAARRVLLDALEAMTDHLPSLILVGAQAVHHHTGKT
                     DLNVPLMTTDADLAIDTRGLADVPEIGAVLRGAGFVPGPNPGHWVAATDVAVDLMVVP
                     HQAGTHRESARAARLAPHDRLTARIARGLEPALVDHERVTIGALEPVDDRTFDLAIAG
                     AAALLVAKVIKISERLGQSDRQPDRLKEKDALDAFRLLQAVETADLVLGLTKHRSEPN
                     AAAVADEAVQILRLHATSPRGRIAHLAASAAGGDATVAPAFAALCNDLLAALR"
     gene            complement(5929..7002)
                     /locus_tag="PLF56_03600"
     CDS             complement(5929..7002)
                     /locus_tag="PLF56_03600"
                     /inference="COORDINATES: protein motif:HMM:NF020897.2"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_function: GO:0003677 - DNA binding [Evidence IEA];
                     GO_process: GO:0006355 - regulation of DNA-templated
                     transcription [Evidence IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="helix-turn-helix domain-containing protein"
                     /protein_id="HOQ52680.1"
                     /translation="MRNDAGPETLLLEDAIRQVRELLPRAWTANLTDGAAPYAGDVLL
                     SVRGPAGVGVTYRVEAKRAGGRSLADTVAALRERQRETGFPVLFVADYIGPATRRALA
                     QAGISYVDATGWVRLVSDDPLILLMGEGAERSPRAPESAAVVRLNGVATSRVIRALSE
                     VEVPVGVRELASVAQVSPGSVSKLLVTLAAEGIVDRDARGALTGVRRRSLLRRWVQDY
                     GYATTNPGLGYWLAPRGLAQVTDRLAKQEGVAVTGSAAARRLLPPDATSVVPLRLFAL
                     YAASPRRLAEQLGLVEADAATANAVIVAPQDQRILPQAGEPVALAPTALVLADLLTLP
                     GRSDAEAEQLMDALAATDPSWKE"
BASE COUNT         1320 a         2565 c         2729 g         1195 t
ORIGIN      
        1 accaccagca tcagcccgga cgccaactcg ggtgccgccc cgatccgtcc gacggcctcg
       61 atcgcgatgt agacaccgac gccgatcatc agcagcaccg ccagccccga ggcgaacacc
      121 tcggcgcggt aggagccgaa ggtgtgcagg ccggtggtgt ccttgcgagt ggccagccgg
      181 gtggcgatca gggcggcgcc gagcgtggtc acgtcagcgg ccacgtgtcc ggcgtccgat
      241 agcagggcca acgaacccga gttccacgcc gccagcagct cgaccacgaa gaagccggcc
      301 accagcacga aggcgacggc cagccgccat cggtgccgtc cggtcgcgcc ggcgtgatcg
      361 tgacgtggac gcctcgaagt gacgtgagcg tcgattacct acggcactac ttcggctctg
      421 aggcgggggt cgaaagcctg gccagcgcat cccctggctc agccggccgc aaccggaccc
      481 tgtcgatcaa gaacttcgag caggttctgg ttccagttcc ttctcgcccg gaccaagacc
      541 gcatcgtcgc tcatctgggt aggcacacgc gtcctggccg tgacgacagc acgcttgatg
      601 ccctggtcgc gggtttcacc gcccgaggga ttgaggagtc gcctctcgtt ccgttcggct
      661 cgctcctcca gttgcagcga gagcctgtcg agctggtgga tggtgagcga taccggagga
      721 tcggcatcta ctcctggggg aaggggatgt tgcggcggga atcggcctcc gccaacgaga
      781 tgggtagcat gcgctacttc acctttccca taccatccct tgtgttcagc aataaccagg
      841 cttgggaagg cgccgtggca ttggccagtc ccgaggatgg cggccacgtg tgttctagtc
      901 gcttctatcc ctacgtgcca cgggagggcg tcgatgtgga cctccgattt ctcctggaat
      961 tcttccgcag tgatcaaggc atcgctctga tgcgccgagc gagcccgggc acccaagtac
     1021 gcaacaagct cctcagccgc tcagccctag agatggccgt tgtgcccttc cctggccgca
     1081 cgatccagga ccgaatcgtg acgcgccgac agatggtcga caaggccacc gacgtcatca
     1141 agcgagctga tgaactcgcg gacgcgatcc tccctgctgc ccggaacgag atcttcaacg
     1201 cgatgcggtg acactgcgcc aggcaacgtc cgcgaccacg acatggtcga gcaaccggag
     1261 tccgacgtga ccgccgtctg cgacctgccc ggttgccgcg cggtcggcgt ccgtcggggt
     1321 cgggtcgccg ccggggtggt tgtgggcgag ggcgatcgcg agaccgtcgt ggcacgacac
     1381 cgcgctgagc acctcccgca cgggcaggcc gcagccactc gcgccaccct tggctacgtc
     1441 ttcgaggtgg cgcagccgca accccgaatc ggccacgagc acgaccaggc gttccacccg
     1501 tgcgccggcg aggcgagccc gggccacttc tgcgatcgtc ccggagtctg ctaggcgagg
     1561 gggctccccg ggtgacccga ggcgtccggc caacgccagc gctgccacca accgggacgc
     1621 cttggcgggg cccagagcgt ccaggcgagg gagctcggga acgccccacc gggccagccc
     1681 ggcgacccca ccgcagcggg cgagcacggc ttgggcgagg gcgacggcgt tgcgtccacg
     1741 cccgccggtt ccgagcagga tggccaccag ttcggcgtcg ctcagtgcgt gtgatccctc
     1801 gcgcgggagg cgctcgcgcg ggaggcgctc gcgcgggcgg tcgtgggcgg gccacgtcag
     1861 gagcggtggg gaggtcatgg gggcagtctc gcgggcgggc acggacctcc acatcggcca
     1921 ccagacacct gtggataacc cagtcggcac gtcacgcgtc ctgctcgccc gcactcccca
     1981 gcgagcgctg acaggcacat cgtcggctcg acaggaagga gtgatcgatg cgcctgcctg
     2041 ctcggcacgg caggatgaac acgaatccca gaggagtggc atggtgaagg cgaacgagac
     2101 caacctcgag ggcatcctcg agggcaagcg gcagtaccag gtgccgctct accagcgcgt
     2161 ctactcgtgg ggtcccaaac agatcgacca gttgtgggat gacctgatcg agatcgctga
     2221 agcgcgtcgg gccgggggta cgcccaccca cttcatcggg tcgctggtgt tggcggccag
     2281 ccccgacaac ggggtggtcg gcgtccacaa gttcctggtc gtcgacggtc agcagcggct
     2341 cacgaccttg tcgatcctgc tggcggccat ccgcgatcat ctgtcagagt ccgacggcca
     2401 ggagcaccgc gaccggatcg acgcccagta tctcgtcaac aagtacgagg cgggcaagcc
     2461 tcccaagctg gtgccgacac aggccgaccg gtcggcgtat ctggcgatcg tgcgtgccag
     2521 cgcggaggca ggcggcgagg acgccgtggg tgcggcgtat cggcggttcc gggtgaagct
     2581 ggccgagatc gacgatcccg aggatcccca cgacatcgcg gccatcgaga acgccgtgct
     2641 ccgtggactc gcgctggtcg cggtcacagc ggaggccggc gacaacgcgc accggatctt
     2701 cgagtcgctc aacaacaccg gtctgcggct cactcagtcg gatctgctca agaaccactt
     2761 gttcatgcgc ctgggtgatc gtgcggagga ggtgtacgag gcgctgtggc gtccgttgga
     2821 ggacaagctc ggcgcggaga acctggagtt gttgttctgg ctggacctcg tgcacaacga
     2881 cgagaaggcc aagcagtcgg acacctacct ggggcagcaa cgccgcttcg acacgctgac
     2941 caccgcggct gaggtcgcgg cggagatccg tcggatcgcc aagctcggcg acgtgctggc
     3001 gaccatcctc aagcccgaac gcgaacagaa ccaggcccta cggaagcggc tcgaacgcat
     3061 ccgggcctgg ggctccacga cggcgtaccc gatcgtgatg caggtgctgg cgcagcgagc
     3121 agcaggacgc gcctccgacg acaaggccat cgcagccctc ctctgcctgg agtcgtactt
     3181 cgttcgcagg atcgtgatcg ggagggcgac cgcaggcttg aaccggacgc tcttgcaggc
     3241 ggtcggcgcc atcaccgagg cgtccgacgt cgaggcggcg ttgcgggatt acctgtcgga
     3301 ggggcgcaag cacttcgcaa cggacgccca ggtgcgcgag gccgtcacga ccgtgccgtt
     3361 ctactggcag gggcgggcgt cccagaagaa gctcatcctg ctgtggctcg aggaagccct
     3421 gcggccgaag gaaccggtcg acgccaagca gctcaccatc gagcacgtgc tgccgcagac
     3481 cttgacggac gccgtgcgcg ccgagttcgc tgccagcttg cctgcggagg ccgaggtggc
     3541 gttcgagcac gagcgcctcg tccacactct ggggaatctc acgctgtccg ggtacaacag
     3601 cgagttgagc aaccggccgt tctcgcagaa gcgcacgatg ctcgccgaga gcggcgtgcg
     3661 catgaaccag acgatcgcgg agcaccagac gtggggtgcg agcgagatca cggaacgtgc
     3721 gcaggcgctg gccgaacgca tcgtggaact gtggcccggt cccaacgaga agctcgtcgg
     3781 gatccgtgac cacgagtccg agttgcgtct ggacgtcgcc gccgtgctgg ccgggattcc
     3841 gtccgggagg tggaccacct acggcgaggt ggcggtggtg gtcggcagcc accccgttcc
     3901 cgtgggttcc gcgatcgcga accatccgat gcccaatcca tggcgggtgc tgcagtcggg
     3961 aggaaccgtc tcggatcagt tccggtggac cgatcggggc cggacggacg atccgcgcga
     4021 cctgctccgt gacgagggcg tcgacttcga agaggcgggc agggccgacc ccgagcggtt
     4081 catggacgcc gtagaactgg catccggggt ggggatggac atcgatgcgt ccaccctgag
     4141 gaggcgtcgg cgccggcagg cacgaaagac caccacggat ccaggagccc tgggcgtcca
     4201 acaggtcgcg ttctgggggt cggtgcagga ctggggccgc gagcatgcca cgcatgcttc
     4261 cttccaccga tcgccaggtc gccgacactg gtactcggtc gagccggcgg gcctcgcact
     4321 ccaggtgaac ctcaacgtga acaccatgaa gccaagggtc tacgtggaga tatacctcga
     4381 ggggggcaag gagcagttcc acgcgctgca caaccagagg aaggaaatcg agggcgacct
     4441 cggctacgcc gtggagtggc gcgagcaacc ggagaagaag gccagccgca tcatccggga
     4501 gcgccccggc gacttccgag acccggcgca ggctgaccag ctggtgcaat ggctcgtcag
     4561 gaccgccgac gacttcgttc gagtgctgca acgactcggc ggatgaggtg cgcggccgac
     4621 tctggacatg ggcaggcaag gacaccgatt ggcatggagc tgcgggggcg tctggcgacg
     4681 ggtgacgtcc ctgccgggac agcaagccgg cctcgagcgg gggcgcggtc gtccctggag
     4741 cgaacaagaa cgcattagac ccacgtccgc aggtggctcc gcgtgaactc tcacgtccca
     4801 gcatgaccac atcgccgcgg accgatgttt cacgtatgtt gcacgacatg gaaaacccgt
     4861 tcgaccggga ttcgttttcc ctagtcagaa cgggtttgtt cgttgtgcgc gagagaggag
     4921 ttgaagctct cttctatagc agataatgac cccctgacaa ggacttatgt ggaccgattg
     4981 gcacttcgtc accttgccgt ggggaatacg tggggaatga ttctgaaccc cagacccgcg
     5041 cggaacacgc gcccaaggct gaccctcagc ataggcggac acgagagggt cgctggcgag
     5101 agttacgttg ctcatcgcag ggctgccagc aggtcgttgc agagggcggc aaacgcggga
     5161 gcgaccgtgg cgtcgcctcc tgcggcggat gctgcgaggt gcgcgatccg gcctctgggt
     5221 gaggtcgcgt gcaagcgcag gatctggacg gcttcatcgg cgacggccgc ggcgttgggt
     5281 tcactgcggt gcttggtcag tccgaggacg aggtcggcgg tctcgactgc ctggaggagg
     5341 cggaaggcgt cgagggcgtc cttctccttg agcctgtccg gttggcggtc ggactgtccg
     5401 agccgttcgc tgatcttgat gaccttggcc accagcaggg cggccgcgcc ggcgatggcc
     5461 aggtcgaacg tgcggtcgtc gaccggttcc agcgccccga tggtgacgcg ttcgtggtcg
     5521 accaaggcgg gctcgaggcc tctggcgatc cgggcggtca gcctgtcgtg gggtgcgagc
     5581 cgtgcggcac gcgccgactc ccggtgcgtg cccgcttgat gcgggacgac catgaggtcg
     5641 acggcgacgt cggtggctgc gacccagtga ccggggttcg ggccgggaac gaagccggca
     5701 ccgcgcagga ccgcgccgat ctcggggacg tccgccagcc cgcgggtgtc gatggcgagg
     5761 tcggcgtcgg ttgtcatcaa gggcacgttg aggtccgtct tgccggtgtg gtggtggacg
     5821 gcttgggcgc cgaccaggat gaggctcggg aggtggtcgg tcattgcttc gagggcgtcg
     5881 aggaggacgc ggcgggcggc gacgtactcg ggggcgacgg cgtccatgtc actccttcca
     5941 cgatgggtcg gttgcggcga gggcgtccat caactgctcg gcctcggcgt ccgaacggcc
     6001 gggcagggtc aacaggtccg ccagcacgag cgctgtgggc gcgagagcga caggttcacc
     6061 cgcttggggg aggatccgct ggtcctgggg ggcgacgatg accgcgttcg cggtcgccgc
     6121 gtccgcctcc accaagccca actgctcagc cagcctgcgg ggcgacgccg cgtaaagagc
     6181 gaacagtcgc aacgggacta ccgacgtcgc gtccggcggc agcagccggc gggcagccgc
     6241 ggaacccgtg accgccacac cctcctgttt agcgagccgg tcggtgacct gggccagccc
     6301 tcgcggcgcg agccagtacc ccaggccagg gttggtggtg gcgtatccgt agtcttgcac
     6361 ccagcgccgc agcaacgagc gacgccggac gccggtcaag gcgccgcggg cgtcccggtc
     6421 gacgatcccc tcagccgcca aagtgacgag cagcttagac accgacccgg gcgacacctg
     6481 cgccacactg gccaactccc ggacaccgac cggcacctcg acctcggaca gggcgcggat
     6541 cacgcgactg gtggccaccc cgttcaaccg gaccaccgca gcggactccg gagcacgagg
     6601 cgagcgctcg gcaccctcac ccatgagcag gatcaacggg tcgtccgaga ccaagcgcac
     6661 ccagcccgtc gcgtcgacgt aactgatccc cgcctgggcc agggcacggc gggtggcggg
     6721 gccgatgtag tccgcgacga acaagaccgg gaagcccgtc tcccgctgac gctcccgaag
     6781 ggctgccacg gtatcggcga gcgacctccc gcccgcccgc tttgcctcga ctctgtaggt
     6841 gacgcccacc cctgccggcc ccctcaccga caacagcaca tcacccgcgt acggagcggc
     6901 gccgtcggtg aggttggccg tccacgcgcg cggcagcagc tccctgacct gacgtatggc
     6961 atcctcaagc agaagtgttt ccgggccagc atcgtttctc atagtacgaa acattctact
     7021 tcaagaaaca gcgagggcga gagtctggct ggccccagag cgcgggtgca gtgtcgcggt
     7081 tgagggcgcg ttcgaactca tcgccggttc ccgctcccgc tctgcatcgc agccccctgt
     7141 tcggtcggac acgccactcg ctctcataac gggcgtctgc tgttctcggg ggacacgatt
     7201 cagagcagac acgggccgcg ccggccaagg gctgatgacg gacccatggt cctgatgggg
     7261 ccccttgctc cgcgactgac actgtggcct tgcgggtgtc gacgccgctc gcgaccggcg
     7321 agtgtcagct gacggcggtg gtggtcatag tgcttgggtg cccattcgag cacgtcggcg
     7381 cgggtcatcc acgacgtgaa gtatggatcc cactgttcgg ggacagccat cccggtctgc
     7441 agctcggcgt cggtggctcg gcgtccccag tcgacgagcc agtcggtgtc gatgtccatc
     7501 caatgctcgg cgcgacgtag cccggagacg cgggcgcctg cgactggccc ggcccagttg
     7561 acccagttgt agggacgcgt caccacatgt ggaacagcag ctcgcggttg gtccagcggg
     7621 tcccccgcgt gggcgccagc agttcctcgg ggtggcagcc gcgatgaggc ggcggtagtc
     7681 gtcgcggacg cgttcaagct cggacgcgat gtcgtcagcg gctggcatcc atccagtgtg
     7741 cgccccgccc gggaccctcc gtcacaggtt gagcgatgcc agaaggctgc ggacgcgtcg
     7801 ttcgacgtc
//
LOCUS       DAOSUG010000069         7808 bp    DNA     linear   ENV 10-JUN-2024
DEFINITION  MAG TPA_asm: Micropruina sp. isolate AMR_MDS_2195
            NODE_4724_length_7808_cov_4.229460, whole genome shotgun sequence.
ACCESSION   DAOSUG010000069 DAOSUG010000000
VERSION     DAOSUG010000069.1
DBLINK      BioProject: PRJNA925477
            BioSample: SAMN32864262
            Sequence Read Archive: SRR4236673
KEYWORDS    WGS; ENV; Metagenome Assembled Genome; MAG; Third Party Data; TPA;
            TPA:assembly.
SOURCE      Micropruina sp. (wastewater metagenome)
  ORGANISM  Micropruina sp.
            Bacteria; Bacillati; Actinomycetota; Actinomycetes;
            Propionibacteriales; Nocardioidaceae; Micropruina.
REFERENCE   1  (bases 1 to 7808)
  AUTHORS   Abdulkadir,N., Saraiva,J.P., Zhang,J., Stolte,S., Gillor,O.,
            Harms,H. and Rocha,U.
  TITLE     Genome-centric analyses of 165 metagenomes show that mobile genetic
            elements are crucial for the transmission of antimicrobial
            resistance genes to pathogens in activated sludge and wastewater
  JOURNAL   Microbiol Spectr 12 (3), e0291823 (2024)
   PUBMED   38289113
REFERENCE   2  (bases 1 to 7808)
  AUTHORS   Abdulkadir,N.
  TITLE     Direct Submission
  JOURNAL   Submitted (24-MAR-2023) Environmental Microbiology, Helmholtz
            Center for Environmental Research -UFZ, Permoserstr 15, Leipzig
            04318, Germany
COMMENT     The annotation was added by the NCBI Prokaryotic Genome Annotation
            Pipeline (PGAP). Information about PGAP can be found here:
            https://www.ncbi.nlm.nih.gov/genome/annotation_prok/
            
            ##Genome-Assembly-Data-START##
            Assembly Method        :: SPAdes v. 3.11.1
            Genome Representation  :: Full
            Expected Final Version :: Yes
            Genome Coverage        :: Not Applicable
            Sequencing Technology  :: Illumina MiSeq
            ##Genome-Assembly-Data-END##
            
            ##Genome-Annotation-Data-START##
            Annotation Provider               :: NCBI
            Annotation Date                   :: 05/04/2023 04:57:10
            Annotation Pipeline               :: NCBI Prokaryotic Genome
                                                 Annotation Pipeline (PGAP)
            Annotation Method                 :: Best-placed reference protein
                                                 set; GeneMarkS-2+
            Annotation Software revision      :: 6.5
            Features Annotated                :: Gene; CDS; rRNA; tRNA; ncRNA
            Genes (total)                     :: 2,776
            CDSs (total)                      :: 2,750
            Genes (coding)                    :: 2,714
            CDSs (with protein)               :: 2,714
            Genes (RNA)                       :: 26
            tRNAs                             :: 24
            ncRNAs                            :: 2
            Pseudo Genes (total)              :: 36
            CDSs (without protein)            :: 36
            Pseudo Genes (ambiguous residues) :: 0 of 36
            Pseudo Genes (frameshifted)       :: 2 of 36
            Pseudo Genes (incomplete)         :: 32 of 36
            Pseudo Genes (internal stop)      :: 2 of 36
            ##Genome-Annotation-Data-END##
FEATURES             Location/Qualifiers
     source          1..7808
                     /organism="Micropruina sp."
                     /mol_type="genomic DNA"
                     /submitter_seqid="NODE_4724_length_7808_cov_4.229460"
                     /isolate="AMR_MDS_2195"
                     /isolation_source="Activated sludge of municipal
                     wastewater treatment plants"
                     /db_xref="taxon:2737536"
                     /environmental_sample
                     /geo_loc_name="Canada"
                     /lat_lon="49.25 N 123.1 W"
                     /collection_date="2015-06-16"
                     /metagenome_source="wastewater metagenome"
                     /note="metagenomic"
     gene            <1..560
                     /locus_tag="PLF56_03605"
     CDS             <1..560
                     /locus_tag="PLF56_03605"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_015071199.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_function: GO:0005524 - ATP binding [Evidence IEA];
                     GO_function: GO:0016887 - ATP hydrolysis activity
                     [Evidence IEA]"
                     /codon_start=3
                     /transl_table=11
                     /product="AAA family ATPase"
                     /protein_id="HOQ52681.1"
                     /translation="RRLVTLVPQTAADLLYLPSVGAECDQADTESDVEPGTTARLLRG
                     LGVIVPEERDPRDLSEGQRLALVLAIQLAARPPVLLLDEPTRGLDYSAKAELRRMIGE
                     LAADGRAVLISTHDVEFAAATSRRTLLMADGELIADGSTAEVLTSSPAYAPQMAKVFA
                     PLQILTPDEVAAGLPATISSRRGTS"
     gene            637..1449
                     /locus_tag="PLF56_03610"
     CDS             637..1449
                     /locus_tag="PLF56_03610"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_004808981.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_function: GO:0022857 - transmembrane transporter
                     activity [Evidence IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="ECF transporter S component"
                     /protein_id="HOQ52682.1"
                     /translation="MTREQGRISVAVVVAIVIGFVAFSWPLFLRPESGMRPEQAPIVF
                     AFVLPIVLATVLSEVAGGRMDARTLAMLGVLAAVGTILRPLSAGTAGFELVFFILILA
                     GRVFGAGFGFALGAITLFTSALITSGVGPWLPYQMLSAGFVGLFAGLLPPAKGKAEIW
                     LLAAYGMVSAFAYGWVMDFSFWPFGIGNDTPISFDPALGPLENLHRFVLYNLATSMGW
                     NLGRALTNAVVIALVGPALLRLLRRSARRAEFTAAFDNPPAVPSSSVELAVE"
     gene            1786..2400
                     /locus_tag="PLF56_03615"
     CDS             1786..2400
                     /locus_tag="PLF56_03615"
                     /EC_number="1.15.1.1"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_002516078.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_function: GO:0004784 - superoxide dismutase activity
                     [Evidence IEA];
                     GO_function: GO:0046872 - metal ion binding [Evidence
                     IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="superoxide dismutase"
                     /protein_id="HOQ52683.1"
                     /translation="MTYTLPDLDYDYGALEPHISAQIMQLHHDKHHAAYVAGANTALE
                     KLAAARESGDFGAINKLEKDLAFHLGGHINHSVFWKNMSPEGGGRPDGEIASAIDEFF
                     GSFEGFQKQFNEVANGLQGSGWSVLVWDTLGKRLNINQLFDQQGNLPVGQLPVLQLDM
                     WEHAFYLQYLNVKGDYVKAWWNVVNWADVTERFTKAQQASSGLF"
     gene            2816..3913
                     /locus_tag="PLF56_03620"
     CDS             2816..3913
                     /locus_tag="PLF56_03620"
                     /inference="COORDINATES: protein motif:HMM:NF019142.2"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="transglycosylase family protein"
                     /protein_id="HOQ52684.1"
                     /translation="MIWSWKLTALSAAVVLAGGAVAATSLDKTVAVTVDGETTSTTGF
                     ANTVADVLAARGITVGSRDVVVPSVDSPIGNGDSVQVQLAKKVTLLVDGKPTEFYTTA
                     TTLDGALATAQVRGLNGADFSVSRSIGIGREGLTVDVTTPKTITLKVAGKSKKITTTA
                     STVAEVLGEQNVGMNVGDRIKPALTAGVDDVASITLTRVAVDTRTVTEKVGYSTVKTK
                     SNKLFTGQTKVTSAGKNGKAKVTYEYTSINGGKATKKVLTRVVLTAPVSKRVTVGTKA
                     GSVSGGGINLARAGMWDRIAQCESGGNWSINTGNGYYGGLQFDFGSWRANGGADFAPR
                     ADQASRAEQITVANRYYAKAGLGPWGCRHAA"
     gene            3943..4707
                     /locus_tag="PLF56_03625"
     CDS             3943..4707
                     /locus_tag="PLF56_03625"
                     /inference="COORDINATES: protein motif:HMM:NF019142.2"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="transglycosylase family protein"
                     /protein_id="HOQ52685.1"
                     /translation="MSRLIKMSALAACTTLLVSGLTALPASADDATGTLTLDLHYAGQ
                     QRSATVTGAATVADAIAQLKLAVDGDDRVSPAVTTPLTDGLSVTIDVVSTSTTVKKNV
                     TIKKKTITKKTKKLYKGHKKVVTKGRDGKATRTYLTTTVNGVSTTAVVSQKITKKVIN
                     KVVKVGTKSTKGLNLARLSKWNKIAKCESGSRWHINTGNGYFGGLQFNLGTWRSVKGQ
                     QFAKYPHKASKAEQITVANRLYAKRGFKPWGCRHVL"
     gene            complement(4897..5385)
                     /locus_tag="PLF56_03630"
     CDS             complement(4897..5385)
                     /locus_tag="PLF56_03630"
                     /inference="COORDINATES: ab initio
                     prediction:GeneMarkS-2+"
                     /note="Derived by automated computational analysis using
                     gene prediction method: GeneMarkS-2+."
                     /codon_start=1
                     /transl_table=11
                     /product="hypothetical protein"
                     /protein_id="HOQ52686.1"
                     /translation="MSWNDFGERLQRALREVTDRSVLIVAAAAELGAGYVQFIGVDDL
                     LVAEASGPEFVTGVATHTAEQPIMLAAGWVAPTRPQPNWSFELALPALTSEYAELAGR
                     CVTALRDVFGIAEPEVLTYRAWREPEQQPPGVTWPPERFDQLDPGENPLILPSLGLRQ
                     VV"
     gene            complement(5390..5860)
                     /locus_tag="PLF56_03635"
     CDS             complement(5390..5860)
                     /locus_tag="PLF56_03635"
                     /inference="COORDINATES: protein motif:HMM:NF040049.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="DUF6301 family protein"
                     /protein_id="HOQ52687.1"
                     /translation="MTWKAMSPGRACDLVDFWAGAQWPRTAEQVQQLGTEVGWTPDDD
                     EFMTNRLDALSEPSVAIITLPSGEVGSFSFWTTDVVREPGEQAEAFLDDHYALLVREG
                     RSRWGRPELTTGDGASARWTLPGGSRAVVSRGPRSVVVDFTTPQYAAVLHDLGE"
     gene            complement(5875..>7808)
                     /locus_tag="PLF56_03640"
     CDS             complement(5875..>7808)
                     /locus_tag="PLF56_03640"
                     /inference="COORDINATES: ab initio
                     prediction:GeneMarkS-2+"
                     /note="Derived by automated computational analysis using
                     gene prediction method: GeneMarkS-2+."
                     /codon_start=3
                     /transl_table=11
                     /product="hypothetical protein"
                     /protein_id="HOQ52688.1"
                     /translation="QVGAGLKAASIGARVAKITSAVADFAIPGGSFLVKGGIHAIPVL
                     SKVFKFADHLPTNLADDLARDGAKLKFGGPGVLDDVVPDLTPTHADRPVSQNLFGGPE
                     VDVPRATPPHSAAPDLDPPGTRPSAQTQPDVDAPAGRPAAQADPEVPAGKAEVPAGKA
                     DEAGTNGQQPAPEATTRPDQAEASTTHETDAPHETTETPATSRPEASAQPEPGTQPEP
                     GTQPEPGTRPAASSEPQNGVHPEAASESATGTRPEATAPPEPSTHPEPGTQPGASTHP
                     EPATAHPEPGTHPGGAPDAAATHVEGGRSAATGIDGQAAGPSTHLDAGAHAGHADPAS
                     AHAPTQAPDGGPLQDRHGRPYTFDADGRRHVPGDPDGTYRDRAGKLRDHSGWIADTNR
                     PGHTDLPFEKATKGTPEDFTVSDAERAAHDGRVSARDAALDGSHLAHDRLSRLVESTG
                     LSPEVLKGSTDDVVRALTRQVDEGTLTVRQRQSVLRALAHDRVAAQMLKNVSERLGDK
                     AAAAVARMRGETSLIGAGGAGAGRFDQVTVGGHPPTLRFYEAKGGDAGLGARTVDGVR
                     HQQGTSGYLDDVLKSDSRLQDSVRQFIEANPDSPITQALKDGTIKVEYDLVQALPGGR
                     IKLTPFVLDPRAFTLPTFK"
BASE COUNT         1213 a         2813 c         2616 g         1166 t
ORIGIN      
        1 cgcggcgcct ggtcaccctg gtgccgcaga cggcggccga cctgctctac ctgcccagcg
       61 tcggagccga gtgcgaccag gccgacaccg agagcgacgt cgagcccggc accaccgccc
      121 ggctgctccg cggcctcggg gtgatcgtgc cggaggagcg cgacccgcgc gacctttccg
      181 aggggcagcg cctcgcattg gtgctggcga tccagctggc cgcccgtccg ccggtgctgc
      241 tgctcgacga gcccacccgc ggcctcgact acagcgccaa ggccgagctg cgccggatga
      301 tcggcgagct ggcggcggac ggccgcgcgg tgctgatcag cacccacgac gtcgagttcg
      361 ccgccgccac cagccggcgc accctgctga tggccgacgg cgagctgatc gccgacggca
      421 gcacggccga ggtgctgacc tcgtcgcccg cctacgcccc ccagatggcg aaggtgttcg
      481 cgccgctgca gatcctcacc ccggacgagg tcgctgccgg cctgcccgcc acgatctcgt
      541 cgcgcagggg gacgtcatga gcaccacagc agagtgccga cgctgcagca ccgcccaccc
      601 cggacgggtg ctcaaggttt cgaccctggg gcgacgatga ccagggaaca gggccgcatc
      661 tcggtcgccg tcgtggtcgc gatcgtgatc ggcttcgttg cgttctcgtg gccgctgttc
      721 ctgcggccgg agtcgggcat gcggccggag caggcgccga tcgtgttcgc gttcgtgctg
      781 ccgatcgtgc tggccaccgt actgtccgag gtggccggcg gacgcatgga cgcccgcacg
      841 ctcgccatgc tgggcgtgct ggccgccgtc ggcacgatcc tgcgtccgct gagtgccggc
      901 acggcgggct tcgagctggt gttcttcatt ctgatccttg ccggacgcgt gttcggcgcc
      961 ggcttcggct tcgcactcgg cgcgatcacc ctgttcacct cggccctgat cacctcgggc
     1021 gtcggcccgt ggctgcccta ccagatgctc tcggccggct tcgtcgggct gttcgccggc
     1081 ctgttgccgc ctgccaaggg gaaggccgag atctggctgc tcgccgcgta cggcatggtg
     1141 tcggccttcg cctacggctg ggtgatggac ttcagcttct ggcccttcgg tatcggcaac
     1201 gacaccccga tctcgttcga tccggccctc ggtccgctcg agaacctgca tcgtttcgtg
     1261 ctgtacaacc tcgccacctc gatgggctgg aacctcggcc gggccctgac caacgcggtg
     1321 gtgatcgccc tggtgggccc ggccctgctg cggctgctgc gccgctccgc ccggcgcgcg
     1381 gagttcacgg ccgccttcga caatccaccg gcagtgccca gcagctccgt tgagttggcg
     1441 gtcgaatgag tcgagccgtc gagcagtcag tgaccgccgg tggaatgacg tcacggcgcg
     1501 cgcgacgact ttcggccgaa gccacgccgc cgtcggcccc ggaggtccac gcgcaccgga
     1561 tgcccctcac accccctcag cggacgcacc gagtggctgc ggccgtgcgc gcggtccgtc
     1621 cagcgtgctg agcgagggcg ggcgaccgcg acgggaacaa gccgctcggg cggtactgcc
     1681 ccaccctgcg gggcctaccg cttgccggcg ttcggaatag agtgaagctg aagcaggttg
     1741 ccgccgataa gggccgcatg ccaccgacca acgaaggaga cccgaatgac gtacacgctt
     1801 ccggacctcg actacgacta cggcgccctc gagccacaca tctcggcgca gatcatgcaa
     1861 ctgcaccacg acaagcatca tgccgcctac gtggccggcg ccaacaccgc gctcgagaag
     1921 ctggccgcgg cccgcgagag cggcgacttc ggcgccatca acaagctcga gaaggacctc
     1981 gccttccacc tcggtggcca catcaaccac tcggtgttct ggaagaacat gtcacctgag
     2041 ggcggcggac gtcccgacgg cgagatcgcc agcgccatcg atgagttctt cggctcgttc
     2101 gagggctttc agaagcagtt caacgaggtg gccaacggcc tgcagggctc agggtggtcg
     2161 gtgctggtct gggacaccct cggcaagcgc ctgaacatca accagctctt cgatcagcag
     2221 ggcaacctgc cggtcggtca gctgcccgtg ctgcaactcg acatgtggga gcacgccttc
     2281 tacctgcagt acctgaacgt gaagggcgac tacgtcaagg cgtggtggaa cgtggtcaac
     2341 tgggccgacg tgaccgagcg cttcaccaag gcgcagcagg cctccagcgg cctgttctga
     2401 gccacctcac ggccagcggc tgagccaatt cgcggcagcg accgagccac ctgacggcag
     2461 cgcccgagct acccacggca gtgcctgagc cagctcggca gcggctgagc cacgcggtag
     2521 cggtcgagcc acctgacggc agcggctgag ccgccgccga gaccgagacc gacggcctcc
     2581 ggagaactcc tccgggggcc gttttcgcac gctgtgacga gcggtggtcg tgttcaccac
     2641 cgtccaaggc gtggacgcag cttcccccac aaaccgcgct gactacgcga tgcacgcatg
     2701 acattcgacg gcactcctga gagatttgct tgatttctca gcttgagtca cactcttctc
     2761 aggttgttct catgaactcc ccagtattgc cgtcgaaact tggagtaacc gcatcgtgat
     2821 ctggtcctgg aagctgaccg cgctcagcgc agccgtcgta ctcgccggtg gtgccgtcgc
     2881 cgcaacgtcc ctcgacaaga ccgtcgccgt caccgtggac ggcgagacca cctcgaccac
     2941 cggcttcgcc aacaccgtgg cggacgtgct cgccgcccgc ggcatcacgg tcggttcgcg
     3001 cgacgtggtg gtgccctcgg tcgacagccc gatcggcaac ggcgacagcg tgcaggtgca
     3061 gctggccaag aaggtcaccc tgctggtgga cggcaagccg accgagttct acaccaccgc
     3121 caccaccctg gacggcgccc tcgctaccgc ccaggtgcgc ggcctgaacg gcgccgattt
     3181 ctcggtgtcc cgttcgatcg gcatcggacg cgaggggctc accgtcgacg tcaccacacc
     3241 caagacgatc actttgaagg tggccggcaa gagcaagaag atcaccacta ccgcgagcac
     3301 cgtggccgag gtgctgggtg agcagaacgt cggcatgaac gtgggcgacc gaatcaagcc
     3361 cgccctgacc gccggcgtcg acgacgtggc gtccatcacg ctcacccggg tcgctgtcga
     3421 caccaggacc gtgaccgaga aggtcggcta cagcaccgtc aagaccaagt cgaacaagct
     3481 gttcaccggc cagaccaagg tcacctcggc gggcaagaac ggcaaggcca aggtcaccta
     3541 cgaatacacc tcgatcaacg gcggcaaggc caccaagaag gtgctcacgc gcgtggtgct
     3601 caccgcgccc gtcagcaagc gcgtcaccgt cggcaccaag gccggctcgg tcagcggcgg
     3661 cggcatcaac ctggcccgcg ccggcatgtg ggaccggatc gcgcagtgcg aatcgggtgg
     3721 caactggtcg atcaacaccg gcaacggcta ctacggcggc ctgcagttcg acttcggctc
     3781 gtggcgtgcc aacggtggcg ccgacttcgc cccccgcgcc gaccaggcca gccgcgccga
     3841 gcagatcacc gtcgccaacc gctactacgc caaagccggc ctgggccctt ggggctgccg
     3901 gcacgccgcc tgaccttcgt ccacacacaa cgggagacca ccatgtcgcg actcatcaag
     3961 atgtcggccc tggccgcctg caccaccctg ctggtcagcg ggctgacggc gctgccggcc
     4021 tcggccgacg acgccaccgg caccctgacg ctcgacctgc actacgcggg ccagcagcgg
     4081 tccgccaccg tgaccggcgc cgccaccgtc gcggacgcca tcgcacagct caagctggcc
     4141 gtcgacggcg acgaccgggt cagcccggcg gtcaccaccc cgctcaccga cggcctgagc
     4201 gtcaccatcg atgtggtcag caccagcacg accgtcaaga agaacgtgac gatcaagaag
     4261 aagacgatca ccaagaagac caagaagctc tacaagggcc acaagaaggt cgtcaccaag
     4321 ggtcgcgacg gcaaggccac ccgcacctac ctgaccacca cggtcaacgg cgtctcgacc
     4381 acggcggtgg tctcgcagaa gatcaccaag aaggtgatca acaaggtggt caaggtcggc
     4441 accaagagca ccaagggcct caacctggcc cggctgtcca agtggaacaa gatcgccaag
     4501 tgcgagtcgg gcagccgctg gcacatcaac accggcaacg gctacttcgg cggcctgcag
     4561 ttcaacctgg gcacctggcg ctccgtgaag ggccagcagt tcgcgaagta cccgcacaag
     4621 gcgtccaagg ccgaacagat caccgtggcg aaccggctct acgccaagcg cggcttcaag
     4681 ccgtggggct gccgccacgt gctctgaccc ccgaaccggt tctcatccgc ccgagagccc
     4741 ggggacgcgt ccacctcacc ggtggaccgt cccactgggc cgctctcgcc ccccgaagag
     4801 ccggccccaa cgaaactggg ctcccaccgc cgggagccca gttcgtcgtt gtggacgcgt
     4861 ggatgcgagc cgcgctcgca acccggacgg cctggttcag accacctgcc gcaaccccag
     4921 ggacggcagg atcaacgggt tctcacccgg gtccagttga tcgaaccgct ccggcggcca
     4981 ggtgaccccg ggcggctgtt gctccggttc acgccaggca cggtaggtga gcacctccgg
     5041 ctcggcgatg ccgaacacgt cgcgcagcgc cgtcacgcac cggccggcca actcggcgta
     5101 ctcgctggtc agggccggca gcgccagctc gaacgaccag ttgggctgcg gacgggtcgg
     5161 cgccacccag ccggcggcca gcatgatcgg ttgctccgcg gtgtgcgttg ccacaccggt
     5221 gacgaactcg ggaccggacg cctcggcgac cagcagatcg tccacaccga tgaactggac
     5281 gtagccggcg ccgagttcgg cggccgccgc gacgatcagc accgaccggt cggtgacctc
     5341 acgcagtgcg cgttgcagcc gttcaccgaa gtcgttccac gacatcggat cactccccca
     5401 ggtcgtgcag gaccgccgcg tactgcggcg tggtgaagtc gaccacgacc gagcgcggcc
     5461 cgcgcgagac cacggcgcgg ctgcccccgg gcagcgtcca acgcgccgag gctccatcgc
     5521 cggtggtcaa ctcgggccgg ccccagcggc tgcggccctc gcggacgagc agcgcgtagt
     5581 ggtcgtcgag aaaggcctcg gcctgctcgc ccggctcgcg cactacgtcg gtcgtccaga
     5641 agctgaacga gccgacctca ccggacggca gcgtgatgat cgccaccgag ggctcgctca
     5701 aggcgtcgag ccggttggtc atgaactcgt cgtcgtcggg agtccagccg acctcggtgc
     5761 ccagttgctg gacctgctcg gcagtccgcg gccattgggc gccggcccag aagtcgacca
     5821 gatcgcacgc ccggccgggg ctcatcgcct tccacgtcat gacttgaacc ctatctactt
     5881 gaatgtcggc agcgtgaagg cacgggggtc gagcacgaac ggcgtgagct tgatccggcc
     5941 gcccggcaag gcctgcacga ggtcgtattc gaccttgatg gtgccgtcct tgagcgcttg
     6001 ggtgatcggc gagtccgggt tggcctcgat gaattggcgc accgaatcct gcagccggga
     6061 atcggacttc agcacatcgt ccaggtaccc gctggtgccc tgctggtgac gaacgccgtc
     6121 caccgtgcgc gcaccgagcc ccgcatcgcc gcccttggcc tcgtagaacc tgagcgtcgg
     6181 cggatgtcca cccacggtga cctggtcgaa ccgtccggcg ccggcacccc ccgcaccgat
     6241 cagcgaggtc tcgccccgca ttctggcgac cgcggccgcg gccttgtcac ccagtcgctc
     6301 cgagacgttc ttcagcatct gggctgcaac ccggtcgtgc gccagtgccc tgagcaccga
     6361 ctgccgctgc cggacggtga gggttccctc gtcgacctgc cgggtgaggg cccgcaccac
     6421 atcgtcggtc gagcccttga gaacctcggg cgacaatccg gtgctctcga ccagccggct
     6481 gagccggtcg tgagccaggt gggagccgtc gagtgcggca tcgcgagcgg acacccggcc
     6541 gtcgtgcgcg gcacgctcgg cgtcgctgac cgtgaagtcc tccggtgttc cctttgtggc
     6601 cttctcgaac gggaggtcgg tgtgaccggg tcggttggtg tcggcgatcc agccgctgtg
     6661 gtcgcgcagc ttgcccgccc tgtcgcggta ggtgccgtcc ggatcaccgg gcacgtggcg
     6721 gcggccgtcc gcgtcgaagg tgtagggccg gccgtggcga tcctggagcg gaccaccgtc
     6781 cggggcctgg gtcggcgcgt gcgcgctcgc cgggtcggcg tgacccgcgt gggctccggc
     6841 gtcgaggtgg gtgctcggcc cagcggcctg gccgtcgatg cctgtcgcgg cggagcgacc
     6901 cccctcgacg tgggtggcgg cggcatcggg cgcgccgccc ggatgggtgc ccggctcggg
     6961 atgggccgtg gccggctccg gatgcgtgga cgcccccggc tgagtgccag gctccgggtg
     7021 ggtggacggc tcgggaggcg cggtcgcctc cggccgagtg cccgtggccg actcactagc
     7081 cgcctccggg tgaacgccat tctgcggctc actggatgcc gccggccgag tgcccggctc
     7141 cggctgcgtg cccggctccg gctgcgtgcc cggctccggc tgggcggagg cctccggccg
     7201 gctggtggcc ggcgtctcgg tggtctcgtg cggcgcatcg gtctcgtgcg tcgtgctggc
     7261 ctcggcctga tcgggcctgg tggtcgcctc gggagccggt tgctgaccgt tcgtcccggc
     7321 ctcatccgcc ttacctgccg gcacctctgc cttgccggcc ggcacctcgg gatcggcctg
     7381 cgccgccggc cgcccggccg gggcgtccac gtccggctgg gtctgggcag agggccgggt
     7441 gcccggagga tcgaggtcgg gagccgccga gtgcgggggc gtggcacgcg gcacgtccac
     7501 ctccgggcca ccgaacaggt tctggctcac cggccggtca gcgtgcgtgg gcgtgaggtc
     7561 gggcacgacg tcgtccagca cgccggggcc gccgaacttg agcttcgcgc cgtcccgtgc
     7621 caggtcgtcc gccaggttgg tgggcaggtg gtcggcgaac ttgaacacct tgctcagcac
     7681 cggaatggcg tggatgccgc ccttgaccag gaacgagcca cccggaatcg cgaaatcggc
     7741 cacggcgctg gtgatcttcg ccacccgtgc cccgatgctc gccgccttca gccccgcccc
     7801 gacctggc
//
LOCUS       DAOSUG010000070         7783 bp    DNA     linear   ENV 10-JUN-2024
DEFINITION  MAG TPA_asm: Micropruina sp. isolate AMR_MDS_2195
            NODE_4738_length_7783_cov_3.958980, whole genome shotgun sequence.
ACCESSION   DAOSUG010000070 DAOSUG010000000
VERSION     DAOSUG010000070.1
DBLINK      BioProject: PRJNA925477
            BioSample: SAMN32864262
            Sequence Read Archive: SRR4236673
KEYWORDS    WGS; ENV; Metagenome Assembled Genome; MAG; Third Party Data; TPA;
            TPA:assembly.
SOURCE      Micropruina sp. (wastewater metagenome)
  ORGANISM  Micropruina sp.
            Bacteria; Bacillati; Actinomycetota; Actinomycetes;
            Propionibacteriales; Nocardioidaceae; Micropruina.
REFERENCE   1  (bases 1 to 7783)
  AUTHORS   Abdulkadir,N., Saraiva,J.P., Zhang,J., Stolte,S., Gillor,O.,
            Harms,H. and Rocha,U.
  TITLE     Genome-centric analyses of 165 metagenomes show that mobile genetic
            elements are crucial for the transmission of antimicrobial
            resistance genes to pathogens in activated sludge and wastewater
  JOURNAL   Microbiol Spectr 12 (3), e0291823 (2024)
   PUBMED   38289113
REFERENCE   2  (bases 1 to 7783)
  AUTHORS   Abdulkadir,N.
  TITLE     Direct Submission
  JOURNAL   Submitted (24-MAR-2023) Environmental Microbiology, Helmholtz
            Center for Environmental Research -UFZ, Permoserstr 15, Leipzig
            04318, Germany
COMMENT     The annotation was added by the NCBI Prokaryotic Genome Annotation
            Pipeline (PGAP). Information about PGAP can be found here:
            https://www.ncbi.nlm.nih.gov/genome/annotation_prok/
            
            ##Genome-Assembly-Data-START##
            Assembly Method        :: SPAdes v. 3.11.1
            Genome Representation  :: Full
            Expected Final Version :: Yes
            Genome Coverage        :: Not Applicable
            Sequencing Technology  :: Illumina MiSeq
            ##Genome-Assembly-Data-END##
            
            ##Genome-Annotation-Data-START##
            Annotation Provider               :: NCBI
            Annotation Date                   :: 05/04/2023 04:57:10
            Annotation Pipeline               :: NCBI Prokaryotic Genome
                                                 Annotation Pipeline (PGAP)
            Annotation Method                 :: Best-placed reference protein
                                                 set; GeneMarkS-2+
            Annotation Software revision      :: 6.5
            Features Annotated                :: Gene; CDS; rRNA; tRNA; ncRNA
            Genes (total)                     :: 2,776
            CDSs (total)                      :: 2,750
            Genes (coding)                    :: 2,714
            CDSs (with protein)               :: 2,714
            Genes (RNA)                       :: 26
            tRNAs                             :: 24
            ncRNAs                            :: 2
            Pseudo Genes (total)              :: 36
            CDSs (without protein)            :: 36
            Pseudo Genes (ambiguous residues) :: 0 of 36
            Pseudo Genes (frameshifted)       :: 2 of 36
            Pseudo Genes (incomplete)         :: 32 of 36
            Pseudo Genes (internal stop)      :: 2 of 36
            ##Genome-Annotation-Data-END##
FEATURES             Location/Qualifiers
     source          1..7783
                     /organism="Micropruina sp."
                     /mol_type="genomic DNA"
                     /submitter_seqid="NODE_4738_length_7783_cov_3.958980"
                     /isolate="AMR_MDS_2195"
                     /isolation_source="Activated sludge of municipal
                     wastewater treatment plants"
                     /db_xref="taxon:2737536"
                     /environmental_sample
                     /geo_loc_name="Canada"
                     /lat_lon="49.25 N 123.1 W"
                     /collection_date="2015-06-16"
                     /metagenome_source="wastewater metagenome"
                     /note="metagenomic"
     gene            complement(407..1651)
                     /locus_tag="PLF56_03645"
     CDS             complement(407..1651)
                     /locus_tag="PLF56_03645"
                     /inference="COORDINATES: protein motif:HMM:NF025019.2"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="DUF4143 domain-containing protein"
                     /protein_id="HOQ52689.1"
                     /translation="MEYLPRVIDDRLSRWLRGRPAVRLEGPRGCGKTITALQHAGSAV
                     HLDANPGMVELAQLDPDRILAGRAPRLVDEWRLAPSLWPQITGRLGDQPLPGRYLLAS
                     SATPLEYRHHTRDAAQLPVLRMRTMSLTERPGIEAHVSLAGLAYGDNVAGVRSRLSYT
                     DLAEHAVRGGWPELLNAPTEQAIEYNTSYLHALAHTDLKTLVLTRHQPDRITDLLTSL
                     ARHTATDASLAGLGHNTAARLGRDTVRSYLDALDRLHAWEPQPAWQPPLRTRARLRHR
                     PRAHLADPALACAALGYDADRLADDPQRFAAIFTAMVIHDLRVYLEGTGAQLYHYKDE
                     TGPAIDAIIEYPDGRWAAVQTSLGDHHVVDAEIALHTLRDDRLDLDQIGRPRFLAIIT
                     GGTQASITRNQTYTIPLATLTA"
     gene            1964..5770
                     /gene="mobF"
                     /locus_tag="PLF56_03650"
     CDS             1964..5770
                     /gene="mobF"
                     /locus_tag="PLF56_03650"
                     /inference="COORDINATES: protein motif:HMM:NF041492.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_function: GO:0003677 - DNA binding [Evidence IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="MobF family relaxase"
                     /protein_id="HOQ52690.1"
                     /translation="MAIGLKKLLAGSGYEYLTRQVAPGDVTSLADYYTAKGETPGRWW
                     GAGPAGMGLAEGDPVTEEQMKRLFGAGVNPVTGEKLGRAYSVFADRPTAFETELERRK
                     QTWQDQHAGARVTKQVRQRLQTDLAIEWHTRDHGRPPAGRRELHSYIAKATSHPRVAV
                     AGFDLTFSPPKSVSALWALAGPELSAAIRAAHTAAVDDALIQAQARVAATRSGHEGVR
                     HIGVRGISAARFDHRDSRAGDPDLHTHVAISAKVQTLTGDWRALDAKPLYAAKVYLSE
                     VYTTSLTARLRDLGLVMAPVGRDGKRPVYEIAGLEPALLARWSSRRARIQAWTADLVD
                     AFEADHERPPTRAERLELAQQATLDTRPPKHQPRSEAEQRATWHAEAVQLLGRAGLDR
                     MLQPRAAAPVVDRAWIAHTAQQVINTVEAERSSWTPWNIRAETLRQVRAAGIRLDQIT
                     ALAEAITGQALAAASIPIRTTRNQPVEPAPLLRPDGTPVYEQPTATRYTSRRILYAER
                     RLVDTAGRLGGRQADHNSVTLALLQAMANREPLNPAQQNLVRDMATSGKRLQLAIAPA
                     GTGKTTAMRALATAWTSSGGTVVGLAPSAAAAEQLRTQLGEAAVADNLAKLAWAIAHH
                     EPLAAAVGPDTLVIIDEAGMADTLTLDHLVTWCLDQGASVRLIGDDQQLGAIGAGGVL
                     RDIATQHGALRLDHVVRFADPAEADASLALRSGDTGALGYYLDHDRVHVVDPDTATSR
                     VLTAWRTDIANGLDALMLAPTRERVAALNAAACAARLDGHRAGRQIPLADHNQASLGD
                     VVITRRNNRDLASSDTAWVRNGDRWRITTVHRDGSIDVQHLRNHNQLTLPADYVRENV
                     ELGYATTIHAAQGVTADTCHGLLTGEESRQQAYTMLTRGRLASHAWLQVDNPDTHISP
                     VDPGLLQPATAVQLLETIIARDAAPASATTLLRQADQPELLLGPAVTCYVDAITYAAE
                     HHLPTDVKEAIDTAGTRHGLDRADAWPTLRSHLLLIAANGHNPVGVLDQAALGPLDHA
                     RDPAAVIDYRLDLTQANHRTRGPLPWLPGIPTQLLDDPTWKDYLSGRYQLTRQLADET
                     RQRVADDPTPARWARDLIGLDPALVADVQLWRAAHQTPDTDLRPTGAPRYSGAEHDAQ
                     RRLERALQTSQADMREWVSKITEAAPACASDPTLPAFAAHLARLAQRRPDLDRQLTEA
                     AALGPLPVEHAADALRYRITHLIHQEDEMARREALSIMRAHQPHPQPPPPMHGPHHGH
                     DRGISI"
     gene            5793..6029
                     /locus_tag="PLF56_03655"
     CDS             5793..6029
                     /locus_tag="PLF56_03655"
                     /inference="COORDINATES: protein motif:HMM:NF024139.2"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_function: GO:0003677 - DNA binding [Evidence IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="helix-turn-helix domain-containing protein"
                     /protein_id="HOQ52691.1"
                     /translation="MASHASEPSQPTPEWLSLQQAALIYGISVDTLRRRIATGALPAS
                     RIGARLIRVRIADLDEVCHPIRVDELPARRRRPW"
     gene            6107..6667
                     /locus_tag="PLF56_03660"
     CDS             6107..6667
                     /locus_tag="PLF56_03660"
                     /inference="COORDINATES: ab initio
                     prediction:GeneMarkS-2+"
                     /note="Derived by automated computational analysis using
                     gene prediction method: GeneMarkS-2+."
                     /codon_start=1
                     /transl_table=11
                     /product="hypothetical protein"
                     /protein_id="HOQ52692.1"
                     /translation="MTLRDELWDVAVDQYGYVTALDAHQLGAAVVELGKLAARGKLTR
                     VSYGLYRFPEWPAGSRDHLMEAVLWTRDPEAALSHDTALDVYELSDINPGRIHVTVPR
                     RSKPLRRRRTPAAYTVHYEDLAPSQRGYWEQIPTVTVPTAIDQCISSGVRPDLVRQAI
                     TQAREQGLIDPQTAARQRSALEGGDS"
     gene            6664..7572
                     /locus_tag="PLF56_03665"
     CDS             6664..7572
                     /locus_tag="PLF56_03665"
                     /inference="COORDINATES: protein motif:HMM:NF020419.2"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="nucleotidyl transferase AbiEii/AbiGii toxin
                     family protein"
                     /protein_id="HOQ52693.1"
                     /translation="MTAELPDRQRSPISARVLNTWLREAQTKTGVGEKRIGWLLASTV
                     AVAALQRAIGSDHQPLFLLKGGLLMEFHLGLSARVTKDVDALFRGELASFESALDAAI
                     AEPWGPFTLQRTTIEEIAGARRLVKPRRFDLKLLIRGAVWRRIKIEVSFPEGRIAEGT
                     EPVPAPNAGFFGITTPDHLAGIAMAYQVAQKLHACTDLDEPPAFINDRVRDVVDLLLI
                     RDRFYPALPPSSVRAACLDVFEARAAEARAVDLPTRRWPPRLVVPNDEWKRAYPALAE
                     SVAISLTLEQAVAELGDWVEAIEQAG"
BASE COUNT         1245 a         2932 c         2546 g         1060 t
ORIGIN      
        1 tcgatcatac gacagccacg aactcggacg gtttcaccca ctgccgggcg atggtgctgg
       61 aaacgcatcg tctacggtga gttgcacagc ttcacccctt ccaggcgccg cgggcctcgg
      121 ctgaggccga cccgttcggc gaaccatccg ccggcgcagg gttggcggca ggacaccatg
      181 cacgcggtca ccaccgacgt cgccactcaa tggcgacaac tccggcggtg gttcgccgcc
      241 cgcacacgcg caccggcggg cccgtcgccg acgaagctga ccgaggtcca tgccggggag
      301 gcctccgatt tgcaccgggt gtaatccgag ggggaggggg gtctgggtct ggtgccggtg
      361 gcgctgtgaa cccagggtca ggtggctcag gggtgggtgg cctgggtcag gcggtgaggg
      421 tggcgagggg gatggtgtag gtctggtttc gggtgatgct ggcctgggtg ccgccggtga
      481 tgatggcgag gaatcggggc cggccgatct ggtcgagatc gagccggtcg tcgcggaggg
      541 tgtggagtgc gatttcggcg tcgacgacgt ggtggtcgcc gaggctggtc tggacggcgg
      601 cccagcggcc gtcggggtat tcgatgatgg catcgatcgc cgggccggtt tcgtccttgt
      661 agtggtacag ctgggcgccg gtaccttcga gatagacccg caggtcgtgg atgaccatgg
      721 cggtgaagat cgccgcgaac cgttggggat cgtcggcgag ccggtcggca tcgtagccga
      781 gggcggcgca ggccagggcg gggtcggcga ggtgggcgcg gggccggtgg cggagccggg
      841 cccgggtgcg gaggggtggt tgccaggcgg gttggggttc ccaggcgtgg agccggtcga
      901 gcgcgtcgag gtaggaccgg acggtgtcgc gtccgagccg ggcagcggtg ttgtggccga
      961 ggccggcgag gctggcgtcg gtggcggtgt gccgggcgag gctggtgagc aggtcggtga
     1021 tccggtcggg ctggtgccgg gtgagcacga gggtcttcag gtccgtgtgg gcgagtgcgt
     1081 gcaggtagct ggtgttgtac tcgatggcct gctcggtggg ggcgttgagg agttcgggcc
     1141 agccgccgcg gacggcgtgt tcggccaggt cggtgtagct gagccggctg cggacgccgg
     1201 cgacgttgtc gccgtaggcg aggccggcca gcgacacatg cgcctcgatg ccggggcgtt
     1261 cggtcaggct catggtccgc atccgcagca cggggagctg ggcggcgtcg cgggtgtggt
     1321 gccggtactc cagcggggtg gctgagctgg ccagcaggta gcggccgggc agcggctggt
     1381 cgccgagccg gccggtgatc tggggccaca gggagggcgc gagccgccat tcgtcgacga
     1441 gccggggcgc ccgtccggcc aggatgcggt cggggtcgag ctgggcgagt tccaccatgc
     1501 cggggttggc gtcgaggtgg acggcgcttc cagcatgctg cagggcggtg atggtcttgc
     1561 cgcacccgcg cggcccttcg agccggacgg cgggccggcc gcggagccag cgggacagcc
     1621 ggtcgtcgat caccctgggc aggtactcca cctcatcgac tgtactgcgg tctgcggaaa
     1681 aatgaacgtc actccacgcc agcaggaacg tcactctgcc gtatccggcg tttgcggtct
     1741 gcggggaatc gggcgatcgt caacccctcg gaacaagttc tggggggatg ttgggggacg
     1801 tgccgttgac aaggccgttc acctctcagc tgtggatcgc gagggtgggg ggaggtgccg
     1861 gttggctaca tcgatggtga catgcctgtg ctgtcctgac ctgatcccca ctccggggtc
     1921 gtcccgcccg tccggcgggg cgtgtgtaag gaagggccgg ctgatggcga tcgggttgaa
     1981 gaagttgctg gccgggtcgg gctatgagta cctgacccgg caggtcgcgc cgggcgatgt
     2041 gaccagcctg gccgactact acaccgccaa gggtgagacc ccgggccggt ggtggggtgc
     2101 cggcccggct gggatggggc tggccgaggg cgacccggtg accgaggaac agatgaaacg
     2161 cctgttcggc gccggggtga acccggtgac cggggagaag ctgggccggg cctactcggt
     2221 gttcgccgac cgccccacgg cgttcgagac cgaactcgag cgccgcaagc agacctggca
     2281 ggaccagcac gccggggcgc gggtgaccaa gcaggtccgg cagcggctac agaccgatct
     2341 ggcgatcgag tggcacaccc gcgaccacgg acgtcccccg gccggacgcc gcgagttgca
     2401 cagctacatc gccaaagcca cctcccatcc gcgggtggcg gtggccgggt tcgacctcac
     2461 cttctcccca cccaaatcgg tgtcggcact gtgggcgctc gccggacccg agctgtccgc
     2521 cgcgatccgg gccgcgcaca ccgccgcggt cgacgacgcc ctgatccagg cccaggcccg
     2581 ggtcgcggcc acccggtccg gccacgaagg cgtccggcac atcggagtgc gcgggatcag
     2641 tgcggcccgg ttcgaccacc gcgactcccg ggccggcgac ccggacctgc acacccatgt
     2701 ggcgatctcg gccaaggtcc agaccctcac cggcgactgg cgggccctgg acgccaaacc
     2761 gctctatgcg gcgaaggtgt acctgtccga ggtgtacacc accagcctga ccgcccggct
     2821 gcgtgacctc ggcctggtca tggcgccggt cggccgggac gggaagcgtc ccgtctatga
     2881 gatcgccggc ctcgagccgg cactgctggc ccgctggtcg tcgcggcggg cccggatcca
     2941 ggcctggacc gccgacctgg tcgacgcgtt cgaggccgac cacgaacggc ccccgaccag
     3001 ggccgaacgg ctcgaactcg cccaacaggc caccctcgac acccgcccgc cgaaacacca
     3061 gccccgctcc gaagcagaac aacgcgccac ctggcacgcc gaagccgtcc agctcctcgg
     3121 ccgcgccggg ctggaccgca tgctgcagcc gcgcgcggcg gcgccggtcg tggaccgggc
     3181 gtggatcgcg cacaccgcgc aacaggtgat caacaccgtc gaggccgaac ggtcctcctg
     3241 gacaccgtgg aacatccgag ccgaaaccct ccgccaggtc cgggccgccg gcatccggct
     3301 cgaccagatc accgccctgg ccgaagcgat caccggccag gccctggccg ccgcctcgat
     3361 cccgatccgc accacccgca accagccggt cgaaccggca ccactgctgc gtcccgacgg
     3421 caccccggtc tacgagcagc ccaccgcgac ccgctacacc tcccggcgca tcctgtacgc
     3481 cgaacgacgc ctcgtcgaca ccgccggacg gctcggcggc cgccaagccg accacaactc
     3541 ggtcaccctc gccctcctcc aggcgatggc caaccgggaa ccgctcaacc ccgcccaaca
     3601 gaacctggtc cgcgacatgg ccacctccgg caagcggctg cagctggcga tcgcccccgc
     3661 cgggaccggg aagaccaccg ccatgcgcgc gctggccacc gcctggacct ccagcggcgg
     3721 caccgtcgtc ggtctcgccc cgtccgccgc cgccgccgaa caactccgca cccagctcgg
     3781 cgaagcagcg gtcgccgaca acctcgccaa actggcctgg gccatcgccc accacgaacc
     3841 actggcggca gcggtcgggc cggacacgct ggtcatcatc gacgaggccg gcatggccga
     3901 cacgctcacc ctcgaccacc tcgtcacctg gtgcctcgac caaggtgcca gcgtccgact
     3961 catcggagac gaccaacagt tgggcgcgat cggcgccggc ggcgtcctgc gcgacatcgc
     4021 cacccaacac ggcgccctac gcctggacca cgtcgtccgg ttcgccgacc ccgccgaagc
     4081 cgacgccagc ctcgccctgc gatccggcga caccggggcg ctcggctact accttgacca
     4141 cgaccgcgtc cacgtcgtcg accccgacac cgccaccagc cgggtcctca ccgcctggcg
     4201 caccgacatc gccaacgggc tcgatgcgct catgctcgcc cccacccgcg aacgggtcgc
     4261 cgccctgaac gcggccgcct gtgctgcacg cctcgacggg caccgtgccg gccgacagat
     4321 cccgctcgcc gaccacaacc aagccagcct gggcgatgtc gtgatcaccc ggcgcaacaa
     4381 ccgcgacctc gccagcagcg acaccgcctg ggtccgcaac ggcgaccggt ggcgcatcac
     4441 caccgtgcac cgcgacggct ccatcgacgt ccaacacctg cgcaaccaca accagctcac
     4501 cctgcccgcc gactacgtcc gcgagaatgt ggaactcggt tacgccacca ctatccacgc
     4561 cgcgcaaggc gtcaccgccg acacctgcca cggactcctc accggcgagg agtcccgcca
     4621 gcaggcctac accatgctca cccgcggccg cctggccagc cacgcctggc tccaggtcga
     4681 caaccccgac acccacatct cccccgtcga ccccggcctg ctccagcccg ccaccgccgt
     4741 ccaactcctc gaaacgatca tcgcccgcga cgccgcgccc gcctccgcca ccaccctgct
     4801 ccgccaggcc gaccagcccg aactgctcct cggccccgcc gtcacctgct acgtcgacgc
     4861 catcacctac gccgccgaac accacctccc caccgacgtc aaagaggcca tcgacaccgc
     4921 cggaacgagg cacggcctcg accgggccga cgcctggccc accctgcgca gccacctcct
     4981 gctcatcgcc gccaacggcc acaaccccgt aggcgtcctc gaccaggccg ccctcgggcc
     5041 gctcgaccac gcccgcgacc ccgccgccgt catcgactac cggctcgacc tcacccaggc
     5101 caaccatcgc acccgcggac ccctgccctg gctgcccggc atccccaccc agctcctcga
     5161 cgaccccacc tggaaggact acctgtccgg ccgctatcaa ctcacccgac aactcgccga
     5221 cgaaacccga cagcgcgtcg ccgacgaccc gaccccggcc cggtgggccc gcgacctcat
     5281 cggcctcgac cccgccctgg tcgccgacgt ccagctgtgg cgcgccgccc accaaacccc
     5341 cgacaccgac ctgcgcccca ccggcgcacc ccgatactcc ggggccgaac acgacgccca
     5401 acgccgcctc gagcgggccc tccagaccag ccaggccgac atgcgcgaat gggtctccaa
     5461 gatcaccgaa gccgcccccg cgtgcgccag cgaccccacg ctgcccgcct tcgccgcgca
     5521 cctggcccgc ctcgcccaac gccgacccga cctcgaccgg cagctcaccg aagcggcagc
     5581 gctcggcccg ctgcccgtcg aacacgccgc cgacgccctc cgctaccgca tcacccacct
     5641 catccaccaa gaggacgaaa tggcccgacg cgaagccctc agcatcatgc gggcgcacca
     5701 gccgcaccca cagccgcccc cgcccatgca cggaccccac cacggtcacg accgcggcat
     5761 cagcatctga ccccgccgag aagggaacgg acatggccag ccacgcatcc gaaccgagcc
     5821 agcccacacc cgagtggctc tccctccagc aagccgctct catctatggg atcagcgtcg
     5881 acacccttcg ccgccgaatc gccaccggcg cgctccccgc atcccggatc ggcgcccgcc
     5941 tcatccgcgt ccggattgcg gatctcgacg aggtctgcca tccgatcagg gtcgacgaac
     6001 tacccgctcg tcgccggcga ccatggtgag cacccacgcc caagtcgcga ctcgaagcca
     6061 gggcgctgat cgacaagact ttactgattt cggtaaagtg ttgtccatga ccttgaggga
     6121 cgagctgtgg gacgtcgctg tcgaccagta cggctacgtc acggctctgg acgcacacca
     6181 gctcggagca gcggtcgtgg agctgggcaa gctcgcagcc cgcggcaagc tgacccgcgt
     6241 gtcgtacggg ctgtacaggt tcccggaatg gccggcgggt tcgcgcgacc acctgatgga
     6301 agccgtgctg tggacccgcg accctgaagc ggcgctcagc cacgacaccg cgctcgacgt
     6361 ctacgaactc tccgacatca accccggccg catccacgtc accgtgcccc gcaggtcaaa
     6421 gccgctgcgc cgccgacgga ccccggccgc ctacaccgtc cactacgagg acctcgcacc
     6481 ctcgcaacgt ggctactggg agcagatccc caccgtcacc gtgccgaccg cgatcgacca
     6541 gtgcatctcc tccggcgtgc gcccggacct ggtccgacaa gccatcacac aggctcgcga
     6601 acagggtctg atcgatccgc agaccgccgc ccgccaacgg tccgccctag aagggggtga
     6661 ctcatgacag ccgaactgcc cgaccggcag cgaagcccca tctcggcgcg ggtgctcaac
     6721 acctggctgc gtgaggccca gacgaagacc ggcgtcggcg agaagcggat cggctggctc
     6781 ctggcttcca cggttgcggt ggccgcgttg cagcgcgcca tcggctccga ccaccagcct
     6841 ctcttcctgc tcaagggtgg gctgctgatg gagttccatc tcgggctgag cgcgcgggtc
     6901 accaaggacg tcgatgccct gttccgcggc gagctggcga gcttcgagtc cgccctcgac
     6961 gccgcgatcg cggagccatg gggaccgttc accctgcaac gcacgacgat cgaggagatc
     7021 gccggtgctc gtcgactggt gaagccccgc cgcttcgacc tcaagctgct gatcaggggc
     7081 gcggtctggc gccggatcaa gatcgaggtc tcgttcccgg aaggccgcat cgccgaaggc
     7141 accgagcccg tcccggctcc gaacgccggg ttcttcggga tcacgactcc cgatcacctc
     7201 gccggcatcg ccatggccta ccaggtcgct cagaagctcc acgcctgtac cgacctcgac
     7261 gagccgccgg cattcatcaa cgaccgggtt cgggacgtgg ttgacctcct gctgatccgg
     7321 gatcgcttct atcccgcact gccgccgtca tccgtccgag ctgcctgcct ggacgtgttc
     7381 gaagcccgcg ccgcggaagc tcgcgcggtg gacctgccaa ctcgccgctg gccgcctcga
     7441 ctggtcgtac ccaatgacga atggaagcgg gcatacccgg ccctcgctga atcggtggcg
     7501 atctcgctca cgcttgagca ggctgtggcc gaactgggcg actgggtcga ggcgatcgag
     7561 caggccggtt aggccggttg gccgccgatc tggtcgagag cgtccttctg cccacacctg
     7621 ctcacctcag cgtctgcgac ccccggagat gttgtgcata tgttgtgcaa ggccccggac
     7681 gtgacgatgg ctctgactat ttaccctagt cagagccatg ttcgtgaagt gcgcgagaga
     7741 ggagttgaac ctccacgtcc tttcggacac acggacctga acc
//
LOCUS       DAOSUG010000071         7736 bp    DNA     linear   ENV 10-JUN-2024
DEFINITION  MAG TPA_asm: Micropruina sp. isolate AMR_MDS_2195
            NODE_4773_length_7736_cov_5.309986, whole genome shotgun sequence.
ACCESSION   DAOSUG010000071 DAOSUG010000000
VERSION     DAOSUG010000071.1
DBLINK      BioProject: PRJNA925477
            BioSample: SAMN32864262
            Sequence Read Archive: SRR4236673
KEYWORDS    WGS; ENV; Metagenome Assembled Genome; MAG; Third Party Data; TPA;
            TPA:assembly.
SOURCE      Micropruina sp. (wastewater metagenome)
  ORGANISM  Micropruina sp.
            Bacteria; Bacillati; Actinomycetota; Actinomycetes;
            Propionibacteriales; Nocardioidaceae; Micropruina.
REFERENCE   1  (bases 1 to 7736)
  AUTHORS   Abdulkadir,N., Saraiva,J.P., Zhang,J., Stolte,S., Gillor,O.,
            Harms,H. and Rocha,U.
  TITLE     Genome-centric analyses of 165 metagenomes show that mobile genetic
            elements are crucial for the transmission of antimicrobial
            resistance genes to pathogens in activated sludge and wastewater
  JOURNAL   Microbiol Spectr 12 (3), e0291823 (2024)
   PUBMED   38289113
REFERENCE   2  (bases 1 to 7736)
  AUTHORS   Abdulkadir,N.
  TITLE     Direct Submission
  JOURNAL   Submitted (24-MAR-2023) Environmental Microbiology, Helmholtz
            Center for Environmental Research -UFZ, Permoserstr 15, Leipzig
            04318, Germany
COMMENT     The annotation was added by the NCBI Prokaryotic Genome Annotation
            Pipeline (PGAP). Information about PGAP can be found here:
            https://www.ncbi.nlm.nih.gov/genome/annotation_prok/
            
            ##Genome-Assembly-Data-START##
            Assembly Method        :: SPAdes v. 3.11.1
            Genome Representation  :: Full
            Expected Final Version :: Yes
            Genome Coverage        :: Not Applicable
            Sequencing Technology  :: Illumina MiSeq
            ##Genome-Assembly-Data-END##
            
            ##Genome-Annotation-Data-START##
            Annotation Provider               :: NCBI
            Annotation Date                   :: 05/04/2023 04:57:10
            Annotation Pipeline               :: NCBI Prokaryotic Genome
                                                 Annotation Pipeline (PGAP)
            Annotation Method                 :: Best-placed reference protein
                                                 set; GeneMarkS-2+
            Annotation Software revision      :: 6.5
            Features Annotated                :: Gene; CDS; rRNA; tRNA; ncRNA
            Genes (total)                     :: 2,776
            CDSs (total)                      :: 2,750
            Genes (coding)                    :: 2,714
            CDSs (with protein)               :: 2,714
            Genes (RNA)                       :: 26
            tRNAs                             :: 24
            ncRNAs                            :: 2
            Pseudo Genes (total)              :: 36
            CDSs (without protein)            :: 36
            Pseudo Genes (ambiguous residues) :: 0 of 36
            Pseudo Genes (frameshifted)       :: 2 of 36
            Pseudo Genes (incomplete)         :: 32 of 36
            Pseudo Genes (internal stop)      :: 2 of 36
            ##Genome-Annotation-Data-END##
FEATURES             Location/Qualifiers
     source          1..7736
                     /organism="Micropruina sp."
                     /mol_type="genomic DNA"
                     /submitter_seqid="NODE_4773_length_7736_cov_5.309986"
                     /isolate="AMR_MDS_2195"
                     /isolation_source="Activated sludge of municipal
                     wastewater treatment plants"
                     /db_xref="taxon:2737536"
                     /environmental_sample
                     /geo_loc_name="Canada"
                     /lat_lon="49.25 N 123.1 W"
                     /collection_date="2015-06-16"
                     /metagenome_source="wastewater metagenome"
                     /note="metagenomic"
     gene            <1..704
                     /locus_tag="PLF56_03670"
     CDS             <1..704
                     /locus_tag="PLF56_03670"
                     /EC_number="1.17.4.1"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_019126614.1"
                     /note="Catalyzes the rate-limiting step in dNTP synthesis;
                     Derived by automated computational analysis using gene
                     prediction method: Protein Homology."
                     /codon_start=3
                     /transl_table=11
                     /product="ribonucleotide-diphosphate reductase subunit
                     alpha"
                     /protein_id="HOQ52694.1"
                     /translation="SIHYGSAEGLDFTNMYFYTVTFHAIAASCRLARERGETFDGFDR
                     SAYASGAYFDKYIGGDWTPRTARVQELFASAGIELPTPDDWRALAAEVAEYGMYNQNL
                     QAIPPTGSISYINHSTSSIHPIVSTIEIRKEGKIGRVYYPAPYLTNDNLEYYRDAYEI
                     GPEAIIDTYAEATKHVDQGLSLTLFFPDNVTTRDLNRAQIHAWKKGIKTLYYIRLRQL
                     ALEGTEVEGCVSCAL"
     gene            1052..2026
                     /gene="nrdF"
                     /locus_tag="PLF56_03675"
     CDS             1052..2026
                     /gene="nrdF"
                     /locus_tag="PLF56_03675"
                     /EC_number="1.17.4.1"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_019174208.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_component: GO:0005971 - ribonucleoside-diphosphate
                     reductase complex [Evidence IEA];
                     GO_function: GO:0004748 - ribonucleoside-diphosphate
                     reductase activity, thioredoxin disulfide as acceptor
                     [Evidence IEA];
                     GO_function: GO:0030145 - manganese ion binding [Evidence
                     IEA];
                     GO_process: GO:0009265 - 2'-deoxyribonucleotide
                     biosynthetic process [Evidence IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="class 1b ribonucleoside-diphosphate reductase
                     subunit beta"
                     /protein_id="HOQ52695.1"
                     /translation="MTQKLTLLRGVQAINWNRISDDKDLEVWDRLTGNFWLPEKVPLS
                     NDIPSWRTLKPHEQLMTTRVFTGLTMLDTIQGTVGAVSLIPDALTPHEEAVYTNIAFM
                     ESVHAKSYSSIFSTLISTAEIDDAFRWSEENEHLQRKAQIVLDFYYGDDPLKRKVAST
                     MLESFLFYSGFYAPMYWSSHAKLTNTADLIRLIIRDEAVHGYYIGYKYQQGLKQADQA
                     RRDELKDYTFDLLYELYDNEESYTEDLYDPLGQTEDVKAFLRYNANKALMNLGYEALF
                     PKAATAVSPAILAALSPNADENHDFFSGSGSSYVIGKAISTEDEDWDF"
     gene            complement(2047..2817)
                     /gene="cobF"
                     /locus_tag="PLF56_03680"
     CDS             complement(2047..2817)
                     /gene="cobF"
                     /locus_tag="PLF56_03680"
                     /EC_number="2.1.1.152"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_002549856.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_function: GO:0043819 - precorrin-6A synthase
                     (deacetylating) activity [Evidence IEA];
                     GO_process: GO:0009236 - cobalamin biosynthetic process
                     [Evidence IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="precorrin-6A synthase (deacetylating)"
                     /protein_id="HOQ52696.1"
                     /translation="MRSELLLIGIGAGDPNWVTVEAIRAIERLDVLFVVLKQDELDEL
                     VAARRALVQRYRDVPPRTVELRDPPRPWRTAEDYPAAVRRWREQRLEQWSAAAQAELG
                     QGQVGGFLVWGDPSLYESTLAIADQLVQASATPIELRVLPGVSAVHALTARHRVPLNR
                     QGRAVQVMPARLLADGLPEGVDDAVVMLDGRQTFAAIDPSGIDIYWGAYLGTPDELLV
                     AGPLEQVRDEIVRVRADALARKGWVFDTYLLRRRLTGG"
     regulatory      2882..3044
                     /regulatory_class="riboswitch"
                     /inference="COORDINATES: nucleotide
                     motif:Rfam:14.4:RF00174"
                     /inference="COORDINATES: profile:INFERNAL:1.1.4"
                     /note="cobalamin riboswitch; Derived by automated
                     computational analysis using gene prediction method:
                     cmsearch."
                     /bound_moiety="adenosylcobalamin"
                     /db_xref="RFAM:RF00174"
     gene            3121..3870
                     /gene="cobA"
                     /locus_tag="PLF56_03685"
     CDS             3121..3870
                     /gene="cobA"
                     /locus_tag="PLF56_03685"
                     /EC_number="2.1.1.107"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_016669019.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_function: GO:0004851 - uroporphyrin-III
                     C-methyltransferase activity [Evidence IEA];
                     GO_process: GO:0019354 - siroheme biosynthetic process
                     [Evidence IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="uroporphyrinogen-III C-methyltransferase"
                     /protein_id="HOQ52697.1"
                     /translation="MSVGEVVLVGGGPGDPDLLTVGGLRALQQADVVLYDHLAPLACL
                     DQAKPGATLIDVGKVPRGCQTAQADINALLVEHARRGASVVRFKGGDPFVLGRGSEEW
                     LACAAAGVPVRVIPGVSSAIAGPELAGIPLTHRGVTQNFSVVSGHVGPDHPASSVNWD
                     AVAHSSGTIVILMGVAHLAEICQGLIRRGLDGDTPAAIVARAAMPDQLVLRGTVATLP
                     ELAAASSVEPPALTVIGAVVGLALEPTATPY"
     gene            complement(4009..5580)
                     /locus_tag="PLF56_03690"
     CDS             complement(4009..5580)
                     /locus_tag="PLF56_03690"
                     /inference="COORDINATES: protein motif:HMM:NF013714.2"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_function: GO:0016491 - oxidoreductase activity
                     [Evidence IEA];
                     GO_function: GO:0050660 - flavin adenine dinucleotide
                     binding [Evidence IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="FAD-binding oxidoreductase"
                     /protein_id="HOQ52698.1"
                     /translation="MAELIRRRDLLRSAGLAGIGAALAGCARPSPESSASSPAKPSPS
                     QSASATGTRAGTLSYDQLARQLSGPLLRPGQSGFQPRNLLYNPRFFSQAGPAAIVEVA
                     SAADVAACVRFAGDGGAALRIRNGGHCYGGWSSGPGLVVNLAKLNTVRVNAGGTTATI
                     GAGALLADVYSQLGAKGVSIGGGSCATVGVTGLTLGGGVGVLARTYGLTCDQLKSAQV
                     VTADGRLRTVNADTDADLFWALRGGGGSFAAVTELTFAVRPAPPVQTFFLQWSLSSAA
                     AVLAAWQEWMPKAPRALWSTCKLLVEPGRGGRALVAGTWIGSGSPAAQINALLAQTPA
                     PARRSTGGGSYAATMLAEAGCSGESATACISRSLTPAERQPFAATSSILQQPLPAAGV
                     QAIVAAVGKGSAVTGMVEGGVSFDAFGGAIADVAPGATAFPWRTALADVQYTATWPYA
                     KAASSATPFDNFVQAGRTALQPWLGNSAYVNYADPKLHDFATAYWGPNLPRLRQVKNA
                     YDPHNVFSFPQSVPL"
     gene            complement(5716..6600)
                     /locus_tag="PLF56_03695"
     CDS             complement(5716..6600)
                     /locus_tag="PLF56_03695"
                     /EC_number="3.-.-.-"
                     /inference="COORDINATES: protein motif:HMM:NF013672.2"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_function: GO:0016787 - hydrolase activity [Evidence
                     IEA];
                     GO_function: GO:0046872 - metal ion binding [Evidence
                     IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="polysaccharide deacetylase family protein"
                     /protein_id="HOQ52699.1"
                     /translation="MPTLPRPALDPCRRSDRSWVAGLAAAAVLFSGCAQVPPANLGPN
                     ALSHDIAVPAPPARAVAAANIQADRSSAKKSPSATPKPPKKRTTTAIAKVNCRKKKCI
                     ALTFDDGPGPFTKKLLATLARTHVRATFFMLGKQVHRYPKVARAVAVAGHSIGVHTWD
                     HRSLPLLSNARIAWEINSTVRIIKKVTKTTPDLLRPPYGATNSRVALAAKRAHLGIAL
                     WNVDTLDWKYRNSARVTAAAVRDTRRGSIILVHDIHPTTVAAIPSVIERLRRKGYTFV
                     TVEQLVGKPKPGQRYRHG"
     gene            complement(6985..7671)
                     /locus_tag="PLF56_03700"
     CDS             complement(6985..7671)
                     /locus_tag="PLF56_03700"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_013160081.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_function: GO:0016491 - oxidoreductase activity
                     [Evidence IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="SDR family oxidoreductase"
                     /protein_id="HOQ52700.1"
                     /translation="MERRTVLITGATRGLGHAIAQTLAPDYDLLIGGRDADTTQRVAA
                     TFPVARPFICDLADPASVTEAVARLGPLDVVVHSAGVTNRDRIEDLTANEWSRVLHLN
                     LIAVAELTRLLLPRLRAQRGHVVTINGGLGLHSGPGWAAYSASKFGLRAFTDALREEE
                     RGMLKVTSIHPGRIDTDMQRTIYELDGRTYHPSDHLAPHSVARAVKYALEAGDEAMVE
                     VLQIRPSAIG"
BASE COUNT         1265 a         2770 c         2544 g         1157 t
ORIGIN      
        1 agtcgatcca ctacggctcc gcggagggcc tcgacttcac caacatgtac ttctacacgg
       61 tcacctttca cgccatcgcg gcgtcctgcc ggctcgcccg cgagcggggc gaaaccttcg
      121 acggcttcga ccgctcggcc tacgcctccg gcgcgtactt cgacaagtac attggcggtg
      181 actggacgcc gcggacggcc cgggtgcagg aactgttcgc gtccgccggc atcgagttgc
      241 ccaccccgga cgactggcgc gcgctggccg ccgaggtggc cgagtacggc atgtacaacc
      301 agaacctgca ggcgatccca cccaccgggt cgatcagcta catcaaccac tccaccagct
      361 cgatccaccc gatcgtgagc acgatcgaga tccgcaagga gggcaagatc ggccgggtct
      421 actaccccgc gccgtacctg accaacgaca acctcgagta ctaccgcgac gcgtacgaga
      481 tcggccccga ggcgatcatc gacacctacg cggaggccac caagcacgtc gaccagggcc
      541 tgtcgctgac cctgttcttt cccgacaacg tcaccacccg cgacctcaac cgggcccaga
      601 tccacgcctg gaagaagggc atcaagaccc tgtactacat ccgactgcgg caactcgccc
      661 tggagggcac cgaggtcgag ggttgcgtca gctgcgctct gtaaccaccg agatcccggg
      721 gcaagcccgg gatgacgaac tcttccacca ccgtcgtcca cccgcccgcc gtcatccacc
      781 ccgcccgccg tcatccaccc cgcccgccgt catccacccc gcccaccgtg atcccggcga
      841 acgccgggat ctcgccaccc cgctcaccga gatcccgggg caagcccggg acgacgaacg
      901 cttccaccac cgccatcccg gcgcacgccg agatcccggg gcaagcccgg gatgacgggg
      961 acgagcggac ccgcaccccg cctcacgtcc ccccgcctca cgtcatcccg gcgaacgccg
     1021 ggatctcacc acccgaaccg aggaaccacc catgactcag aaactgaccc tgctgcgcgg
     1081 cgtccaggcg atcaactgga accgcatctc cgatgacaag gacctcgagg tctgggaccg
     1141 gctgaccggc aacttctggc tgcccgagaa ggtgccgctg agcaacgaca taccgagctg
     1201 gcgcaccctg aaaccgcacg agcagctgat gaccacccgg gtgttcaccg gcctgaccat
     1261 gctcgacacc atccagggca cggtcggcgc ggtctcgctg attccggacg ccctcacccc
     1321 gcacgaggag gccgtctaca ccaacatcgc gttcatggaa tcggtgcacg ccaagagcta
     1381 ctcgtccatc ttctcgacgc tgatctcgac cgcagagatc gatgacgcct tccgttggag
     1441 cgaggagaac gagcacctgc aacgcaaggc gcagatcgtc ctcgacttct actacggcga
     1501 cgacccgttg aagcgcaaag tggccagcac gatgctggag tcgtttctgt tctactcagg
     1561 tttctacgcc ccgatgtact ggtcgagcca cgccaagctg accaacaccg ccgacctgat
     1621 ccggctgatc atccgcgacg aggcggtgca cggctactac atcggctaca agtaccagca
     1681 gggcctcaag caggccgacc aggcccgccg cgacgaactc aaggactaca ccttcgacct
     1741 gctctacgag ctgtacgaca acgaagagtc ctacaccgag gacctctacg acccgctcgg
     1801 acagaccgag gacgtcaagg cgttcctgcg ttacaacgcc aacaaggcct tgatgaacct
     1861 cgggtacgag gcgctgttcc ccaaagcggc caccgccgtc tcaccggcga tcctggccgc
     1921 cctgtccccc aacgccgacg agaaccacga cttcttctcc ggctccggct cgtcctacgt
     1981 gatcggcaag gcgatcagca ccgaggacga ggactgggac ttctgagccg gacgtgcctg
     2041 cccggctcag ccgcccgtca gccggcgccg cagcaggtag gtgtcgaaca cccagccttt
     2101 gcgcgccagc gcgtcggccc ggacgcgcac gatctcgtcg cgcacctgct cgagcgggcc
     2161 agcgaccagc agctcgtcgg gtgtgccgag gtaagcgccc cagtagatgt cgatgccgct
     2221 cggatcgatc gcggcgaagg tctgccggcc gtccagcatc accacggcgt cgtccacccc
     2281 ctcgggcagg ccgtcggcca gtagccgggc cggcatcacc tgcacggcgc gtccctgccg
     2341 gttcaacggg acgcggtgcc gtgcggtcag tgcgtgcacc gcgctcaccc cgggaagcac
     2401 ccgcagctcg atcggcgtcg ccgaggcctg caccagctgg tcggcgatcg ccagcgtgct
     2461 ttcgtacagc gacgggtcgc cccacaccag gaagccgccc acctgcccct ggcccagctc
     2521 ggcctgcgcg gcggcgctcc actgctccag ccgctgttcc cgccagcgcc gcacggccgc
     2581 cggatagtcc tccgccgtgc gccacggacg gggcggatcg cgcaactcga cggtgcgcgg
     2641 cggcacgtcg cggtaccgct gcaccagcgc acgccgggcg gccaccagct cgtccagctc
     2701 gtcctgtttg agcacgacga acagcacgtc gagccgttca atggcccgga tcgcctccac
     2761 cgtcacccag ttcggatcac cggcgccgat gccgatcagc agcagctcgc tgcgcattcc
     2821 gctcccttcc gcacaacccg ggccgtgtcg ttgcgcggcc cggccgacct attctttcgc
     2881 gattcgccgg tccgccgtgg gcagctgtgt gaaactggtc agcgtagaaa gtgccgagga
     2941 actccggtgc gccgacaagg caattccggg gcggtcccgc cactgtgacc gggtcgcgcg
     3001 tgcggaccgc aagtcaggaa ctcgccttct gccgagcgat gagattcggg cgaggacacc
     3061 tgaggaggct cccttgaaca cagcctggct gcgcgccacc cacgagcagt ggagcgccgt
     3121 atgagcgtcg gcgaagtagt gctggtcggt ggcgggcctg gcgatccgga cctgctgacc
     3181 gtcggcgggc tgcgtgccct gcaacaggcg gacgtggtgc tgtacgacca cctggcgccg
     3241 ctggcctgcc tcgaccaggc caagccgggc gcgaccctga tcgacgtggg caaggtaccg
     3301 cgcggctgcc agaccgccca ggccgacatc aacgccctgc tggtcgagca tgcccggcgc
     3361 ggggccagcg tcgtccgatt caagggcggc gacccgttcg tgctcggccg gggcagcgag
     3421 gaatggctgg cgtgcgccgc ggccggcgtg cccgtccggg tcattcccgg cgtgtcatcg
     3481 gcgatcgccg gacccgaact ggccggcatt ccgctcaccc accgcggcgt cacccagaac
     3541 ttctccgtcg tgtcggggca cgtcggcccc gaccatccag cctcgagtgt caattgggac
     3601 gccgtcgcgc acagttcggg caccatcgtg atcctgatgg gcgtggcaca cctggccgaa
     3661 atctgtcagg gcctgatcag gcgcggactg gacggcgaca ctcccgccgc gatcgttgcg
     3721 cgagcggcga tgcccgacca gctggtgctg cgcggcaccg tcgcgacgct gcccgagctg
     3781 gccgcagcct catctgtgga accgcccgcc ctcaccgtga tcggtgcggt cgtcggactc
     3841 gctctcgaac cgaccgccac accctactga cgcgctgcgg ggcgcagatc cgatcgccgg
     3901 tcggtgtcgg tggtgaccct gaggggacca tcccgcgacg atcgtcgatg cctatcgagg
     3961 attctcggcg catgccgcag caacggaagg tggcggctgt gcggtcactc acagcggcac
     4021 cgactggggg aacgagaaca cattgtgcgg atcgtaggcg ttcttcacct gcctgagccg
     4081 gggcaggttg gggccccagt aggcggtcgc gaaatcgtgc agtttcgggt cggcgtagtt
     4141 cacgtaggcg ctgttgccca gccagggttg cagggccgtg cgcccagcct gcacgaagtt
     4201 gtcgaagggc gtggcgctcg aagcggcctt ggcgtagggc caggtcgcgg tgtactgaac
     4261 atcggcgagc gcggtgcgcc acgggaaagc ggtggcgccg ggcgccacgt cggcgatggc
     4321 gcccccgaag gcgtcgaacg acaccccgcc ctcgaccatg ccggtgaccg cggagccctt
     4381 gccgacggcc gcaacgatcg cctgcacgcc cgccgcgggt agcggctgct gcagaatcga
     4441 ggacgtcgca gcgaacggct gccgctccgc cggcgtcagc gacctgctga tgcaggcggt
     4501 ggccgactcg cccgaacagc cggcctcagc gagcatggtg gccgcgtaac tgccgccacc
     4561 tgtggagcgc cgcgccggcg ccggggtctg cgccagcagg gcgttgatct gcgcggccgg
     4621 cgagcccgaa ccgatccagg tgccggccac cagcgcgcgt ccgccacggc cgggctcgac
     4681 cagcagcttg caggtgctcc acagcgcgcg gggcgccttc ggcatccatt cctgccaggc
     4741 ggccagcacc gccgccgcgg atgacaggct ccactgcaga aagaacgtct gaacaggcgg
     4801 ggccgggcgc accgcgaacg tcagttcggt gaccgccgcg aagctgccgc ctccaccacg
     4861 cagcgcccag aacaggtcgg catcggtgtc ggcgttcacc gtgcgcaggc gtccgtctgc
     4921 ggtcaccacc tgggcggatt tgagctggtc gcaggtcagt ccgtacgtgc gggccaggac
     4981 cccgaccccg ccgcccagcg tcagcccggt cacccccacg gtggcgcagg agccgccgcc
     5041 gatcgagaca cccttggcgc cgagttggct gtagacgtcg gccagcagcg ccccggcgcc
     5101 gatcgtcgcg gtcgtgccgc ccgcgttcac ccggacggtg ttcagcttcg ccaggttcac
     5161 caccagaccg ggcccggacg accagccgcc gtagcaatga cctccgttgc ggatccgcag
     5221 cgccgctccc ccgtcgccgg cgaaccggac gcacgcggcg acgtccgcgg cgctggcgac
     5281 ctcaactatc gccgccggcc cggcttggga gaagaagcgg gggttgtaaa gcaggttgcg
     5341 cggctggaag ccactctgcc cgggtcgcag cagcgggccg gacagctgcc gcgccagctg
     5401 gtcgtaggac agcgtgccgg cgcgggtgcc ggtggccgag gccgactgcg acggcgacgg
     5461 cttggccggt gagctggcgg acgactccgg cgacggacgg gcacaacccg ccagggcggc
     5521 gccgatcccg gccaggccgg cgctgcgcag caggtcgcgg cgcctgatca gctcggccac
     5581 ggacccgtcc accccatctg gtcggttgct cgacaccact gcggaccgtc ggcgacgacc
     5641 gcagcctcgg gcgtcgacca gccggtgttc acgagaccag ccgtgccggg gtgcggatcc
     5701 ggccccagcc accggctacc catggcggta ccgctggccg ggcttgggct tgccgaccaa
     5761 ctgctcgacg gtgacgaagg tgtagccctt tcgccgcagc cgctcgatca cgcttggaat
     5821 cgcggccacc gtggtggggt gaatgtcgtg caccaggatg atcgagcctc gccgggtgtc
     5881 acgcaccgcg gctgccgtca cccgcgccga gttgcggtac ttccaatcga gggtgtccac
     5941 gttccacagc gcgatcccga ggtgcgcccg cttggcggcc agcgcgaccc gactgttcgt
     6001 cgccccgtag ggaggccgca gcaggtcggg ggtggtcttg gtcaccttct tgatgatccg
     6061 gaccgtcgag ttgatctccc acgcgatcct ggcgttcgac aacaggggca gcgagcgatg
     6121 gtcccaggtg tgcacgccga tcgaatgccc tgcgacggcc acggcacgag cgaccttcgg
     6181 atacctgtgt acctgctttc ccagcatgaa gaaggtggcc ctgacatgcg tgcgcgccag
     6241 cgtggccagc agtttcttgg tgaacggccc agggccgtca tcgaaggtga gcgcaatgca
     6301 cttcttcttg cgacagttga ccttcgcgat cgctgtggtc gtgcgcttct tgggtggctt
     6361 cggggtggct gagggcgact tcttcgcgga cgaacggtcg gcctggatgt tcgcggccgc
     6421 aaccgcccgg gcgggtgggg ccggcaccgc gatgtcgtgg ctgagagcgt tcgggccgag
     6481 gttggccggg ggcacctggg cgcatccaga gaacaggacg gcggctgcgg ccagcccagc
     6541 gacccaggat ctgtcgcttc ggcggcacgg gtcgagggca gggcggggaa gcgttggcat
     6601 ggtcggctcc gtgatgggga aacttcgaga atgccgcaag tcttgcctcg ggcactcaca
     6661 caacactcac ttctgacgat ccgggctgtg acatcttgtt gtgcggcgtg tgtgcaggca
     6721 ccgcgcgttt gtctcctgcg tcgtgggacg accctgccgt ccaggagcct gctgaacaat
     6781 catgtgccgg ctgcggcgca cccggcgggt gatctgagcc ccccatatct gggccagcat
     6841 gtcctatacg ccctccagat ctggtgggct cagctcatcc cgccgggcac gtcctcgccc
     6901 ggcacagcat tgttcagcag actcccaggt tcgcgccggc gcgcacccgg accgccgcgc
     6961 accgtaccaa cgtccgcgca aggatcaacc aatggcgctg ggacgaatct gcagcacctc
     7021 caccatcgct tcgtcgccgg cctccaacgc gtacttgacc gctcgcgcaa ccgaatgcgg
     7081 cgccaggtga tcggacgggt ggtaggtgcg tccgtccagt tcgtagatcg tgcgctgcat
     7141 gtcggtgtcg attcgtccgg gatggatcga ggtcactttc agcatgccgc gctcctcttc
     7201 gcgaagagcg tcggtgaacg cgcgcaggcc gaatttggat gccgaatagg ccgcccagcc
     7261 gggccccgaa tgcagtccga gaccgccgtt gatcgtgacc acatgccccc gctgggcccg
     7321 caaccggggc agcagcagcc gagtcaactc agccacggcg atcaggttca ggtgaagcac
     7381 gcgcgaccac tcgttcgcag tgaggtcctc gatccggtcc cggttggtga caccggccga
     7441 gtgcaccacg acgtcgagtg ggccgagccg cgcgacggcc tcggtaaccg atgccggatc
     7501 ggccaggtcg cagatgaacg gacgcgcgac gggaaacgtg gcagcgaccc gctgggtagt
     7561 gtcggcatca cgtccgccga tgagcaggtc gtagtcgggc gcgagggtct gcgcgatggc
     7621 gtgcccaagc ccgcgggtgg ccccggtgat cagtacggtt cgacgctcca tagccgcgac
     7681 cctagcctcg cgctttcatg agggtggggt gtgacgcggt ggtttgaaca gcgaaa
//
LOCUS       DAOSUG010000072         7719 bp    DNA     linear   ENV 10-JUN-2024
DEFINITION  MAG TPA_asm: Micropruina sp. isolate AMR_MDS_2195
            NODE_4786_length_7719_cov_4.087944, whole genome shotgun sequence.
ACCESSION   DAOSUG010000072 DAOSUG010000000
VERSION     DAOSUG010000072.1
DBLINK      BioProject: PRJNA925477
            BioSample: SAMN32864262
            Sequence Read Archive: SRR4236673
KEYWORDS    WGS; ENV; Metagenome Assembled Genome; MAG; Third Party Data; TPA;
            TPA:assembly.
SOURCE      Micropruina sp. (wastewater metagenome)
  ORGANISM  Micropruina sp.
            Bacteria; Bacillati; Actinomycetota; Actinomycetes;
            Propionibacteriales; Nocardioidaceae; Micropruina.
REFERENCE   1  (bases 1 to 7719)
  AUTHORS   Abdulkadir,N., Saraiva,J.P., Zhang,J., Stolte,S., Gillor,O.,
            Harms,H. and Rocha,U.
  TITLE     Genome-centric analyses of 165 metagenomes show that mobile genetic
            elements are crucial for the transmission of antimicrobial
            resistance genes to pathogens in activated sludge and wastewater
  JOURNAL   Microbiol Spectr 12 (3), e0291823 (2024)
   PUBMED   38289113
REFERENCE   2  (bases 1 to 7719)
  AUTHORS   Abdulkadir,N.
  TITLE     Direct Submission
  JOURNAL   Submitted (24-MAR-2023) Environmental Microbiology, Helmholtz
            Center for Environmental Research -UFZ, Permoserstr 15, Leipzig
            04318, Germany
COMMENT     The annotation was added by the NCBI Prokaryotic Genome Annotation
            Pipeline (PGAP). Information about PGAP can be found here:
            https://www.ncbi.nlm.nih.gov/genome/annotation_prok/
            
            ##Genome-Assembly-Data-START##
            Assembly Method        :: SPAdes v. 3.11.1
            Genome Representation  :: Full
            Expected Final Version :: Yes
            Genome Coverage        :: Not Applicable
            Sequencing Technology  :: Illumina MiSeq
            ##Genome-Assembly-Data-END##
            
            ##Genome-Annotation-Data-START##
            Annotation Provider               :: NCBI
            Annotation Date                   :: 05/04/2023 04:57:10
            Annotation Pipeline               :: NCBI Prokaryotic Genome
                                                 Annotation Pipeline (PGAP)
            Annotation Method                 :: Best-placed reference protein
                                                 set; GeneMarkS-2+
            Annotation Software revision      :: 6.5
            Features Annotated                :: Gene; CDS; rRNA; tRNA; ncRNA
            Genes (total)                     :: 2,776
            CDSs (total)                      :: 2,750
            Genes (coding)                    :: 2,714
            CDSs (with protein)               :: 2,714
            Genes (RNA)                       :: 26
            tRNAs                             :: 24
            ncRNAs                            :: 2
            Pseudo Genes (total)              :: 36
            CDSs (without protein)            :: 36
            Pseudo Genes (ambiguous residues) :: 0 of 36
            Pseudo Genes (frameshifted)       :: 2 of 36
            Pseudo Genes (incomplete)         :: 32 of 36
            Pseudo Genes (internal stop)      :: 2 of 36
            ##Genome-Annotation-Data-END##
FEATURES             Location/Qualifiers
     source          1..7719
                     /organism="Micropruina sp."
                     /mol_type="genomic DNA"
                     /submitter_seqid="NODE_4786_length_7719_cov_4.087944"
                     /isolate="AMR_MDS_2195"
                     /isolation_source="Activated sludge of municipal
                     wastewater treatment plants"
                     /db_xref="taxon:2737536"
                     /environmental_sample
                     /geo_loc_name="Canada"
                     /lat_lon="49.25 N 123.1 W"
                     /collection_date="2015-06-16"
                     /metagenome_source="wastewater metagenome"
                     /note="metagenomic"
     gene            123..1565
                     /locus_tag="PLF56_03705"
     CDS             123..1565
                     /locus_tag="PLF56_03705"
                     /inference="COORDINATES: ab initio
                     prediction:GeneMarkS-2+"
                     /note="Derived by automated computational analysis using
                     gene prediction method: GeneMarkS-2+."
                     /codon_start=1
                     /transl_table=11
                     /product="hypothetical protein"
                     /protein_id="HOQ52701.1"
                     /translation="MSFRQLEADVLNQDPRLDVPLALADPARLESDADELAQRAALLV
                     ADGDPLAGADAYQRAVELARRAELAPTRWLPWELAQVQALSLGGRIEQAMTRAGEVAV
                     AARRASEPTLFAEAALAVASPWVPLGADARQAQLMINEALDWLPRDQAALRVRLIEGS
                     LRAGMAGDATMLGRLGDVEPELMQQSAGPDPAVALDALRALHALTWPRRQPPTVRLEL
                     AQRIAVMAARAGGAEAELEALRLAVTAHIELADRLAAAAAAEHYGRRAAAAGSALHQW
                     WAALRSELLAALAGRTTIAGRHAARAAGLQHEIDPETVQVALHERQLSDALRDGRLAE
                     LAAAMDGLDDDMSSHDPLYQIAGAAIAAAVGEQPTHDYLAQLLKSVRGTFRAGAGAAL
                     CVLALGQVPPSPVLGEALAVELQALSGCWIPIGGSAGVGPADAYLARLIELRGGSRDE
                     VVRRRSQATSLAHKFAPDWVRFTEQFPTKE"
     gene            1568..1954
                     /locus_tag="PLF56_03710"
     CDS             1568..1954
                     /locus_tag="PLF56_03710"
                     /inference="COORDINATES: ab initio
                     prediction:GeneMarkS-2+"
                     /note="Derived by automated computational analysis using
                     gene prediction method: GeneMarkS-2+."
                     /codon_start=1
                     /transl_table=11
                     /product="hypothetical protein"
                     /protein_id="HOQ52702.1"
                     /translation="MPKGTIEDYADDSVTSFVLRWNDWSTADDRQQIYARAWKLDHSS
                     ALGFITAMASDRNDKVPAHIVHYLEKHPEFSPVFLKAFKRVKKNNWRFGAVVNQFDFS
                     FAPAVTLTESRKAGIVMLYVVGDPTV"
     gene            complement(2039..2248)
                     /locus_tag="PLF56_03715"
     CDS             complement(2039..2248)
                     /locus_tag="PLF56_03715"
                     /inference="COORDINATES: protein motif:HMM:NF036450.2"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="DUF5302 domain-containing protein"
                     /protein_id="HOQ52703.1"
                     /translation="MGSIPSQEVTMPDASDDNADTKAAFKAALDRKNQAAQQGGIDHT
                     DAERGSAKNTHAQGGKRQFRRKSGG"
     gene            complement(3217..4113)
                     /locus_tag="PLF56_03720"
     CDS             complement(3217..4113)
                     /locus_tag="PLF56_03720"
                     /inference="COORDINATES: protein motif:HMM:NF024803.2"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="MerR family transcriptional regulator"
                     /protein_id="HOQ52704.1"
                     /translation="MSTIPPDGFGEGSVYTIKQAAQLTGVSSSTLRAWERRYPLPAPH
                     RSSSGYRLYDDAALIRLRVMQRLVQAGWAPRNAAAVALDGAHESDDLPEPRDFVVAVE
                     AGLPPSRREAVLTALFHSCPLPDLIESWLMPMLRELGEAWRSGRVTIWQEHAVATAVL
                     RRLHAAFEALPSLDGPLILTGIPDGSHHELGLAAFDVLAKRTAFEVAYLGPGLPSDGW
                     VQASAAQTPAAVVIAVPTLQDVPAARKTLAALAAMPQAPLLTAGGSQQDAVADLALPL
                     GHSLNGGVERLVGLLGRRQQSR"
     gene            4167..5795
                     /gene="idi"
                     /locus_tag="PLF56_03725"
     CDS             4167..5795
                     /gene="idi"
                     /locus_tag="PLF56_03725"
                     /EC_number="5.3.3.2"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_013862671.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_function: GO:0004452 - isopentenyl-diphosphate
                     delta-isomerase activity [Evidence IEA];
                     GO_process: GO:0008299 - isoprenoid biosynthetic process
                     [Evidence IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="isopentenyl-diphosphate Delta-isomerase"
                     /protein_id="HOQ52705.1"
                     /translation="MLHRLASGDAVVLLDQQGNAIGFAPRLDVHTDATPLHLAFSSYL
                     FDESGRLLLTRRALHKRTWPGVWTNSCCGHPLPQESQQEAVRRRVEEELGLSIEGLTL
                     VLPDFRYRAVDASGVVENELCPVWVGRVSAGELRPDPDEVAEYAWVPWRDLVSSIAAT
                     PQAFSPWAVLQVPQVAALVAADGTPLVDRLDDLVAAVPDEAATVAAVDALVADELTSL
                     TQQWQSWVPDVEVLPADLPEWLNQLAALGGKRFRPRLCHWAYLACGGRPGPAYDNLVR
                     AAAALELLHLFALVHDDVMDESSSRRGAPSAHVQAAGWHRAAGAGGDPDTFGRNLAIL
                     LGDLAHARATRLADELPDDLRATWHELCLELIAGQRADLTGAAAGRRDREHAERIARL
                     KSGSYSVWRPLQLGALAAGADVAQLERLQAFGDHIGRAFALRDDLLGLWGDPAVTGKP
                     AGDDLISGKATVILALANETLTGADAELLQHPQGRVVEALAALQRAGIDQQVERLIGA
                     EVRAARTVLAGGGLQADGTEGLLDLASKIAWRQA"
     gene            5792..7267
                     /gene="crtI"
                     /locus_tag="PLF56_03730"
     CDS             5792..7267
                     /gene="crtI"
                     /locus_tag="PLF56_03730"
                     /EC_number="1.-.-.-"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_013862672.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_function: GO:0016166 - phytoene dehydrogenase activity
                     [Evidence IEA];
                     GO_process: GO:0016117 - carotenoid biosynthetic process
                     [Evidence IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="phytoene desaturase family protein"
                     /protein_id="HOQ52706.1"
                     /translation="MNSVVVVGAGLAGLSAACHLVGRGYRVTVLEAADRPGGRAGAIE
                     QDGFTFDSGPVVLTMRGLLNDAFEAAGSSVEDHLRLQRLDPAYRARFHDGSVLHVRHG
                     HEAMHDEIATHCGAADAKAFDEFVVWLKALYAAELPHFIDANFNSPLDLLRHPVAAAK
                     LLQLGGFGRLGPSIEKRFRDERLHRMFSFQAMYAGLPPALALALYAVITYMDSIEGVW
                     FPEGGMRAMGPAMAEAITAAGGEVRCSTPVERVLRRPDGSVTGVRLADGSALSADAVV
                     VTVDLPVAYDTLLDLQPPRVVRRKPHYSPSCVVWHAGLAGEPEADAVHHTIHFGQQWQ
                     GAFDDLMVRGTLMQDPSRFVCRPSLDDRAAAPEGTQTMYVLEPTPNLVDGELDWGATS
                     RAAMRERLYGFVAESGYPTDVLTERLVTPQDWLEEGMAAGTPFALAHTFMQTGPFRPS
                     NVRREAPGLVFAGSGTTPGVGIPMVLISGKLAAQRVDEVLR"
     gene            7323..>7719
                     /locus_tag="PLF56_03735"
     CDS             7323..>7719
                     /locus_tag="PLF56_03735"
                     /inference="COORDINATES: protein motif:HMM:NF012705.2"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="squalene/phytoene synthase family protein"
                     /protein_id="HOQ52707.1"
                     /translation="MSPSADQIARGLRRSAELTRQHGTTYWWGTLLLTRQQARDVAAV
                     YAVCRLADDLVDEQPDPVRAEADLTAFRDEFYAAWDGAPTDNDVLAAASEVVRRRGIP
                     RHCFDRFFAAMALDLTRTSWASWPDLRDGY"
BASE COUNT         1125 a         2617 c         2805 g         1172 t
ORIGIN      
        1 ccgcgagaac gaccaccagc ggcgccaggc cgcgatgacg ctgcggcatg gcttggtagc
       61 tcacgacgct gatacctgcc tgaccatcga cgacatacgg ctcattgagt caactcccgg
      121 gggtgtcctt ccggcagctc gaagccgacg tgctcaatca ggacccgcgg ctggacgtcc
      181 cgctggccct ggccgacccc gcccggctgg aaagcgacgc ggacgagctc gcgcagcggg
      241 ccgcactgct ggtcgccgac ggcgacccgt tggcgggggc cgatgcctac cagcgggccg
      301 tcgaactggc tcggcgtgcg gaactcgccc ccactcgctg gctgccctgg gagctggcgc
      361 aggtgcaggc tctcagcctc ggtgggcgta tcgaacaggc gatgacccgg gccggcgagg
      421 tagccgtcgc ggcccgccgg gcgtccgaac cgaccctgtt cgccgaggcc gcgttggcgg
      481 tggcgagccc gtgggtgccg ctgggcgccg atgctcgcca ggcgcaactg atgatcaatg
      541 aggccctgga ctggctgccg cgcgaccagg cggcgctgcg cgtccggctg atcgaggggt
      601 ctctgcgcgc gggaatggcc ggcgatgcca ccatgctggg ccgcctgggc gacgtggagc
      661 cggagttgat gcagcagtcc gcggggcccg atcctgcggt ggcgctggac gccctgcgcg
      721 ccctgcatgc gctcacctgg ccgcgccggc agccgccaac ggtgcggctc gaactggcgc
      781 agcggatcgc ggtgatggcc gcccgcgcgg gtggcgccga agccgagttg gaggcgctgc
      841 gcctggccgt caccgcgcac atcgaactgg ccgaccggct ggccgccgcg gccgccgccg
      901 agcattacgg acggcgcgcc gccgccgccg gatcggcgtt gcatcagtgg tgggcggcac
      961 tgcgcagcga gttgctcgca gccctggccg gacggacgac gatcgcgggg cggcacgccg
     1021 cccgggccgc tggcctgcag catgagatcg accccgagac cgtccaggtg gccctgcatg
     1081 agcggcaact cagcgatgcc ctgcgcgacg gcaggctcgc cgaactcgcc gcggcgatgg
     1141 acggcctcga cgacgacatg tcgagccacg acccgctgta ccagatcgcc ggtgcggcta
     1201 tcgcggcggc tgtgggggag cagcccaccc acgactatct ggcccagctg ctgaagagcg
     1261 tccggggcac gttcagggcg ggggccgggg cggcgctttg cgtgctcgct ctggggcagg
     1321 tgcccccgtc gccggtgctg ggcgaggcgc tggcggtgga gctgcaggcg ttgagcggct
     1381 gctggatccc gatcggtggc tcagccggcg tgggcccggc cgatgcctac ctggccaggt
     1441 tgatcgaact gcgcggcggt tcgcgcgacg aggtcgtccg ccgacggtcg caggcgacct
     1501 cactcgccca caagttcgcg cccgattggg tgcggttcac cgagcagttc cccacgaagg
     1561 agtgactatg cccaaaggca ccatcgagga ctacgcggac gacagcgtca ccagctttgt
     1621 gctgcgctgg aacgactggt cgaccgcgga cgaccggcag cagatctacg ccagggcctg
     1681 gaagctcgac cacagcagtg cgctcggctt catcaccgcc atggccagtg accgcaacga
     1741 caaggtgccc gcccatatcg tgcactacct cgagaagcat cccgagttct cgccggtctt
     1801 cttgaaggcg ttcaagcgag tcaagaagaa caactggcgc ttcggagccg tggtgaacca
     1861 gttcgacttc tcgttcgctc cggcggtgac gctgaccgag agtcgcaagg ccggcatcgt
     1921 gatgctgtac gtggtcggcg atccaacggt ctgacccccc gggggccctc gctgcgcggt
     1981 gatcagtgga gtgcttgcga ggtgtccggg cttcggacga agcctggatc cgtgcttgtc
     2041 agccgcccga cttgcgccgg aactgccgct tgccgccctg ggcgtgggtg ttcttcgcgc
     2101 tgccccgctc ggcgtcggtg tggtcgatgc cgccctgttg ggcggcctgg ttcttgcgat
     2161 cgagcgctgc cttgaaggcg gccttggtgt cggcgttgtc gtcgctggcg tcgggcatgg
     2221 tgacctcctg gctgggaatg ctgcccactg tagcgagtga agcggacggt gccgccccag
     2281 cgggacgggg ttgcacaggg agccgccggg gcgacgcaag tatgagcggg gccgggggct
     2341 gcggcagtga atggagttgg ggactcatca cccgccatga atggggttgg ggactgtaag
     2401 tcatcacccg ccaggaccag aacgtgacca attcgatgag cgtgaacagg atcagggacc
     2461 gacggtgaga ccaggatgcg ttcgtcagcg cggttggggt ttgtgacgat ctcgagatcg
     2521 ctgcccgctg gcgggaagac ccgtccactt tcggcttgca gcattcgatc ggctcgatga
     2581 gcgggccgcg cgcaggatag gaaccgacag tgaggtctgt gacgatctcg agatcgctgc
     2641 ccgctggcgg gaagacccgt ccactttcgg gttgcaacac gaaatcgtcg aggcatcccg
     2701 ccatcgagtg gcgatctcga gatcgctcgg cgactggtct cgacgttcta ccagcgaaag
     2761 ggctggaccg acaccaggag agcgattaga cccaattaaa cccgacgcca agaatgacga
     2821 ttgaacccgg caccggacgc aggattgaag gggagtgggg gtgcgccccg tcagggtgtg
     2881 gtcgggcgtc gggcaactcg ccgcgggagc ttgcggagtc gttgaggctg atggtgcggg
     2941 cgggacgggg tgcaccccca ctccgcggca accaacgcaa ccaacgaggt ctcgacaagc
     3001 tcgaccaatg gaatatcagc gtcgtggcag gggtgctggg taagatcccg gacgttcgtc
     3061 gggatacgga ataggtggtc tcgaccaagg gtggttcgac gctcgatcaa cggaatgtca
     3121 gcgtcgcggc agggccgctt gggagatccc ggcgttcgcc gcgatgccgg aagcaagggt
     3181 ctcgaccagc gaagtctccg cgctcgacga aaacccctac cggctctgct ggcggcggcc
     3241 gagcagcccg accagcctct ccacgccgcc gttcagcgag tggccaagtg gtagcgccag
     3301 atcggccacg gcgtcctgtt ggctgccgcc ggcggtgaga agtggggcct gcggcatggc
     3361 ggcgagcgcc gccagagttt tccgtgccgc cgggacgtcc tgcagcgtcg gtacggcgat
     3421 caccaccgcc gcgggcgttt gggcagcgct cgcctgcacc cacccgtccg acggcaggcc
     3481 agggcccaga tacgcgacct cgaacgcggt ccgcttggcc agcacgtcga acgccgcgag
     3541 cccgagctcg tggtggctgc cgtccggaat gccggtcagt atcaacggcc cgtcgaggct
     3601 cggcagcgcc tcgaacgccg cgtgcaaccg ccgcagcacc gcggtggcca cggcatgctc
     3661 ctgccagatc gtcacccggc cggaccgcca cgcctcgccc aactcgcgca gcatgggcat
     3721 cagccagctc tcgatcaggt cggggagcgg acagctgtgg aacagtgcag tgagcaccgc
     3781 ctcgcgccgg ctcggcggca acccggcctc gacggcgacc acgaagtcgc gcggttcggg
     3841 caggtcgtca ctctcatggg caccgtccag ggcgacggcg gctgcgttgc ggggagccca
     3901 accggcctgc accagccgct gcatgacacg taaccgaatc agggcggcgt cgtcatagag
     3961 tcggtaacca ctggacgagc ggtggggtgc gggcagcggg tagcgacgtt cccaggcgcg
     4021 cagggtggac gaactgaccc cggtcagttg tgccgcctgc ttgatcgtgt acacgctgcc
     4081 ctctccgaac ccgtctggcg gaatcgtact cacccgctaa ccttgcacaa agcttgaaca
     4141 gccgtaccga tcaggaggaa tgcgtcgtgc ttcaccgtct ggcatccggc gacgcggtgg
     4201 tgctgctcga tcagcagggc aatgccatcg gcttcgcacc ccggcttgac gtacacacgg
     4261 atgcgacgcc gctgcacctc gccttttcga gctacctgtt cgatgagtcc ggacggttgt
     4321 tgctcaccag acgcgcgctg cacaaacgca cctggccggg ggtctggacg aactcgtgtt
     4381 gcgggcaccc gctgccgcag gagagccagc aggaggccgt ccgacgccgc gtcgaagaag
     4441 agctcgggct gagcatcgag ggcctcaccc tggtgctgcc cgactttcgc taccgagcgg
     4501 tcgacgccag cggggtggta gagaacgagt tgtgcccggt ctgggtcgga cgggtcagcg
     4561 ccggcgagct ccgtccggac cccgacgagg tcgccgagta cgcctgggtg ccgtggcgcg
     4621 acctggtcag ctcgatcgcc gcgacgccgc aggccttcag cccatgggcc gtcctccagg
     4681 tgccgcaggt ggccgcgctg gtcgccgccg acggcacccc gttggtcgac cgcctcgacg
     4741 atttggtcgc cgccgtgccc gacgaagcgg cgacggtcgc tgcggtcgat gcattggtcg
     4801 ccgacgagct gaccagcctc acccagcagt ggcagtcgtg ggtgcccgac gtcgaggtgc
     4861 tgcccgccga cctgcccgaa tggctgaatc aactcgcggc gctgggcggc aagcggttcc
     4921 gtccacggct gtgtcactgg gcgtacctgg cctgcggcgg acgtccgggc ccggcgtacg
     4981 acaacctggt gcgcgccgcc gcagccctcg agttgctgca cctgttcgcc ctggtccacg
     5041 acgacgtgat ggacgaatcg tcctcgcgcc gcggcgcccc gtccgcccac gtgcaggcgg
     5101 ccggctggca ccgggcggcc ggcgccggcg gtgaccccga cacgttcggc cgcaacctgg
     5161 cgattctgct cggcgatctg gcgcacgcga gggcgacccg gctggccgac gagttgcccg
     5221 acgacctgcg cgccacctgg cacgaactgt gcctggagct gatcgccggc cagcgtgccg
     5281 acctgaccgg ggcggcggcc ggacgacgcg accgtgagca tgccgagcgc atcgcgcggc
     5341 tcaagtcggg ctcgtactcg gtgtggcgtc cgctgcagct gggtgccctg gccgccggcg
     5401 ccgatgtcgc gcaactcgag cggttgcagg ccttcggcga ccacatcggc cgcgcgttcg
     5461 ccctgcgcga tgacctgctg gggctgtggg gcgatcccgc ggtgaccggc aagccggccg
     5521 gcgacgacct gatcagcggc aaggccacgg tgattctggc cctcgccaac gaaaccctca
     5581 ccggtgcgga cgcggagctg ttgcagcatc cgcagggtcg tgtcgtcgag gcgctcgccg
     5641 ccctgcaacg tgcgggcatc gaccagcagg tggagcgcct gatcggtgcc gaggtgcggg
     5701 ctgcgcgcac ggtgctggcc ggcggcgggc tgcaagccga cggcaccgaa gggctgctcg
     5761 acctggcgtc gaagatcgcg tggcggcagg catgaactct gtggttgtgg tcggcgcggg
     5821 gctggccggg ctgtcggccg cctgccacct ggtgggccgc ggctaccggg tcaccgtgct
     5881 cgaggcggcg gatcgtccgg gcggacgggc gggcgcgatc gagcaggacg gcttcacctt
     5941 cgatagcggg ccggtggtgc tcacgatgcg cgggctgctc aacgatgcct tcgaggcggc
     6001 cggttcgagc gtcgaggatc acctgaggct gcagcgcctc gatccggcct atcgggcgcg
     6061 ctttcacgac ggatccgtgc tgcatgtgcg gcacggccac gaggcgatgc acgacgagat
     6121 cgccacccac tgcggggccg cggacgccaa ggccttcgac gagttcgtgg tgtggctcaa
     6181 ggcgttgtac gccgccgagt tgccgcactt catcgacgcc aacttcaact caccgctcga
     6241 cctgctgcgg caccccgtcg cggcggcgaa gctgctgcag ctgggggggt tcggccggct
     6301 cggcccgtcc atcgagaagc ggttccgcga cgagcggctg catcgcatgt tctcgttcca
     6361 ggccatgtac gcggggctgc cgccggcgct ggcgctggcg ctgtacgcgg tgatcaccta
     6421 catggactcg atcgagggcg tctggttccc cgagggcggc atgcgggcga tgggcccggc
     6481 gatggccgaa gcgatcaccg ccgccggcgg tgaggtgcgc tgcagcacgc cggtcgagcg
     6541 ggtgctgcgc cgtccggacg gctcggtcac cggcgtccga ttggccgacg gttcggcgct
     6601 cagtgcggac gcggtggtgg tcaccgtcga cctgccggtc gcctacgaca cgctgctcga
     6661 cctgcaaccg ccgcgggtgg tgcgccgcaa gccgcactac tcgccctcgt gcgtggtgtg
     6721 gcatgccggg ttggccggcg agcccgaggc cgatgccgtg catcacacca tccacttcgg
     6781 gcagcagtgg cagggcgctt tcgacgacct gatggtgcgc ggcaccctga tgcaggaccc
     6841 gtcccggttc gtctgccggc ccagcctgga cgaccgggcg gccgctcccg agggcaccca
     6901 gaccatgtac gtgctcgagc cgacgcccaa cctcgtcgac ggcgaactgg actggggggc
     6961 cacgtcccgc gcggcgatgc gcgagcggct gtacgggttc gtggccgagt ccggctaccc
     7021 caccgacgtg ctcaccgagc ggctggtcac cccgcaggac tggctcgagg agggcatggc
     7081 cgccggcacc ccgttcgcgc tggcgcacac gttcatgcag accgggccgt tccgcccgtc
     7141 caacgtgcga cgggaggcgc ccggcctggt cttcgccggc tcgggtacga cgcccggggt
     7201 gggtattccg atggtgctga tctcgggcaa gctggccgcg cagcgggtcg acgaggtgct
     7261 gcggtgagcg gcagcatgcc tccggcgagg cgtcgggtcg ttcggcgaag cggggtgtcg
     7321 tgatgagccc gtcggccgac cagatcgccc gcggtttgcg gcgcagtgcc gaactcaccc
     7381 ggcagcacgg caccacctac tggtggggaa cgctgctgct gacccgccag caggcccgcg
     7441 acgtggccgc ggtctatgcg gtgtgccggc tggccgacga cctggtcgac gagcaacccg
     7501 atccggtgcg agccgaggcc gacctgaccg ccttccgcga cgagttctac gccgcctggg
     7561 acggtgcacc caccgacaac gacgtgctgg ccgccgcgag cgaggtcgtc cggcggcggg
     7621 ggattccgcg gcactgcttc gaccggttct tcgccgcgat ggccctcgac ctgacccgca
     7681 cgtcgtgggc gtcgtggccc gacctgcgcg acggctaca
//
LOCUS       DAOSUG010000073         7698 bp    DNA     linear   ENV 10-JUN-2024
DEFINITION  MAG TPA_asm: Micropruina sp. isolate AMR_MDS_2195
            NODE_4799_length_7698_cov_4.835536, whole genome shotgun sequence.
ACCESSION   DAOSUG010000073 DAOSUG010000000
VERSION     DAOSUG010000073.1
DBLINK      BioProject: PRJNA925477
            BioSample: SAMN32864262
            Sequence Read Archive: SRR4236673
KEYWORDS    WGS; ENV; Metagenome Assembled Genome; MAG; Third Party Data; TPA;
            TPA:assembly.
SOURCE      Micropruina sp. (wastewater metagenome)
  ORGANISM  Micropruina sp.
            Bacteria; Bacillati; Actinomycetota; Actinomycetes;
            Propionibacteriales; Nocardioidaceae; Micropruina.
REFERENCE   1  (bases 1 to 7698)
  AUTHORS   Abdulkadir,N., Saraiva,J.P., Zhang,J., Stolte,S., Gillor,O.,
            Harms,H. and Rocha,U.
  TITLE     Genome-centric analyses of 165 metagenomes show that mobile genetic
            elements are crucial for the transmission of antimicrobial
            resistance genes to pathogens in activated sludge and wastewater
  JOURNAL   Microbiol Spectr 12 (3), e0291823 (2024)
   PUBMED   38289113
REFERENCE   2  (bases 1 to 7698)
  AUTHORS   Abdulkadir,N.
  TITLE     Direct Submission
  JOURNAL   Submitted (24-MAR-2023) Environmental Microbiology, Helmholtz
            Center for Environmental Research -UFZ, Permoserstr 15, Leipzig
            04318, Germany
COMMENT     The annotation was added by the NCBI Prokaryotic Genome Annotation
            Pipeline (PGAP). Information about PGAP can be found here:
            https://www.ncbi.nlm.nih.gov/genome/annotation_prok/
            
            ##Genome-Assembly-Data-START##
            Assembly Method        :: SPAdes v. 3.11.1
            Genome Representation  :: Full
            Expected Final Version :: Yes
            Genome Coverage        :: Not Applicable
            Sequencing Technology  :: Illumina MiSeq
            ##Genome-Assembly-Data-END##
            
            ##Genome-Annotation-Data-START##
            Annotation Provider               :: NCBI
            Annotation Date                   :: 05/04/2023 04:57:10
            Annotation Pipeline               :: NCBI Prokaryotic Genome
                                                 Annotation Pipeline (PGAP)
            Annotation Method                 :: Best-placed reference protein
                                                 set; GeneMarkS-2+
            Annotation Software revision      :: 6.5
            Features Annotated                :: Gene; CDS; rRNA; tRNA; ncRNA
            Genes (total)                     :: 2,776
            CDSs (total)                      :: 2,750
            Genes (coding)                    :: 2,714
            CDSs (with protein)               :: 2,714
            Genes (RNA)                       :: 26
            tRNAs                             :: 24
            ncRNAs                            :: 2
            Pseudo Genes (total)              :: 36
            CDSs (without protein)            :: 36
            Pseudo Genes (ambiguous residues) :: 0 of 36
            Pseudo Genes (frameshifted)       :: 2 of 36
            Pseudo Genes (incomplete)         :: 32 of 36
            Pseudo Genes (internal stop)      :: 2 of 36
            ##Genome-Annotation-Data-END##
FEATURES             Location/Qualifiers
     source          1..7698
                     /organism="Micropruina sp."
                     /mol_type="genomic DNA"
                     /submitter_seqid="NODE_4799_length_7698_cov_4.835536"
                     /isolate="AMR_MDS_2195"
                     /isolation_source="Activated sludge of municipal
                     wastewater treatment plants"
                     /db_xref="taxon:2737536"
                     /environmental_sample
                     /geo_loc_name="Canada"
                     /lat_lon="49.25 N 123.1 W"
                     /collection_date="2015-06-16"
                     /metagenome_source="wastewater metagenome"
                     /note="metagenomic"
     gene            complement(<1..1183)
                     /locus_tag="PLF56_03740"
     CDS             complement(<1..1183)
                     /locus_tag="PLF56_03740"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_008600173.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_function: GO:0005524 - ATP binding [Evidence IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="ATP-binding cassette domain-containing protein"
                     /protein_id="HOQ52708.1"
                     /translation="MAVISFDRVSVTYAGAGAPVLDRVSFELREGDLCLAIGPTGSGK
                     STLLGAINGLVPHFTGGTLAGRVLVDGRDTATHRPRDLADVVGYVGQDPLRGFVTDTV
                     EDEIAYGMEQLGIAPVAMRKRVEETLDLMGIADLRRRPLVELSGGQQQRVAIAAVLAA
                     QPRILVLDEPTSALDPTAAQDVLAAIGTLVHDVGLTVVLAEHRLERVMQAADSMLYLP
                     GDGRVVHGPPAQVLAHASITPPLAELARVLGWPEVPLSVREARRRVASDDVLPTPPDR
                     VPVSTEPVALRTRDLEVRYGQLVAVNAVDLDLVAGTVTSLLGRNGAGKSSLLWALQGA
                     LTCAGSIDVAGQDPRRLDAAQARQETVSADTLEMTSRLIEKKLKDFGVEVRVVLASPG
                     PV"
     gene            complement(1174..2367)
                     /locus_tag="PLF56_03745"
     CDS             complement(1174..2367)
                     /locus_tag="PLF56_03745"
                     /inference="COORDINATES: protein motif:HMM:NF014420.2"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="CbiQ family ECF transporter T component"
                     /protein_id="HOQ52709.1"
                     /translation="MAAEGTQALTNSLHPWAWWCWAISIGIAVNGTTNPLLLLLIATA
                     MVVVVMLRRSDAPWAKSVRVYLLLALFVIGMRLFFRILLGGSAGETVLFTLPSVPLPD
                     WAAGIQLGGVVTAEAVVATLYESLRLAVMLLCIGAANALANPRQALRSVPAALYEAST
                     AVVIALSVAPQLIESIQRVRRARRLRGASTTNLRALGTVAMPVLADAIDRSLSLAAGM
                     ESRGFARTSGLPTRGTLPLMLLSSMAATLGVFLLLGSTYWQVALWLMVLGMVGAGIGL
                     KAAGARLKVTTYRPHPWRSTETLVALSGVLCAVIVLGLGWLDPDALSPALAALLDPLA
                     LSPTTNPLVWPTVSAPMLLVIALVLAPLPLTRQRRATAVESTRNDTRVRLLRPRTNEP
                     EPTWR"
     gene            complement(2340..3260)
                     /locus_tag="PLF56_03750"
     CDS             complement(2340..3260)
                     /locus_tag="PLF56_03750"
                     /inference="COORDINATES: ab initio
                     prediction:GeneMarkS-2+"
                     /note="Derived by automated computational analysis using
                     gene prediction method: GeneMarkS-2+."
                     /codon_start=1
                     /transl_table=11
                     /product="hypothetical protein"
                     /protein_id="HOQ52710.1"
                     /translation="MKKRFLALALAAFAVSACAPASVGAAQTSAAQSTAQQASVPQAP
                     SLPQAASCSGVWVVVDFGSLGGGTSTGCATSYATGLAALRSAGFAPVVEDGFVFKLNG
                     KPSKPDINKAYWSYWHATQQSDGDWSSWSYSNLGAGGFKPKRGTAEGWHYVSISGGNS
                     GPGAKPPAASSSPSPTPTKTTSKPTPKPSKSTNTPKASPSATRTASRTASAKATSSAT
                     ATATGKASAKATAATSASAASTTTATTTEAPGNDPSGIGTDVAAQEPSATAAASGNAG
                     SPVGAIVVGSVVVLAGGGLGGWWLLKGRKP"
     gene            complement(3257..4855)
                     /locus_tag="PLF56_03755"
     CDS             complement(3257..4855)
                     /locus_tag="PLF56_03755"
                     /inference="COORDINATES: ab initio
                     prediction:GeneMarkS-2+"
                     /note="Derived by automated computational analysis using
                     gene prediction method: GeneMarkS-2+."
                     /codon_start=1
                     /transl_table=11
                     /product="hypothetical protein"
                     /protein_id="HOQ52711.1"
                     /translation="MHPVLKRVTAVGAAAALAVSTLTLSAPHAQAAEPNATKAANFLV
                     KNLPTSSDGAAASMTAALGLATTNDCTYAPATRTLVKQIEKNAKSYLYSGKKLNQARA
                     ANLAITVTSLGLDPKKFAGYNLVSLITKSLPADGQIGGSKSAFSQSLGLIALKRAKAT
                     IPVTLLTNLLSQQGDNGAFGYEFPAGTFNADPDTTAMGTLALKAIGQLKPQLSSATTW
                     ATDHQTSEGYWEAYSPVDSTGLVASALKATGGQDAAVAKAKAWLGGVQNADGGFPNAL
                     DAGTDSNVMATANALYLITGTSLLDVSFNLGKCPKNPPSLPKATTSCTGVWVLVDRGN
                     GQDTVRCATKFGNGLDALKSAGFSFAKDKSGLLTRINGFPQTLVPWTPTSKYWSYWYA
                     NPKADGSWGEWQEYQLGAADSKPAKGAAEGWYWKVYTGDATTTAFTRPPKGYSSTGNV
                     TIAGKTKVGQTLTATPGTWTPTPTKVVLQWYRSGKAISKATKATYKLTKSDVGKTITV
                     KVTASGSGLQTVSRTSTATAKVTK"
     regulatory      complement(4933..5070)
                     /regulatory_class="riboswitch"
                     /inference="COORDINATES: nucleotide
                     motif:Rfam:14.4:RF00174"
                     /inference="COORDINATES: profile:INFERNAL:1.1.4"
                     /note="cobalamin riboswitch; Derived by automated
                     computational analysis using gene prediction method:
                     cmsearch."
                     /bound_moiety="adenosylcobalamin"
                     /db_xref="RFAM:RF00174"
     gene            complement(5086..6189)
                     /locus_tag="PLF56_03760"
     CDS             complement(5086..6189)
                     /locus_tag="PLF56_03760"
                     /inference="COORDINATES: protein motif:HMM:NF024640.2"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="hypothetical protein"
                     /protein_id="HOQ52712.1"
                     /translation="MALIRRVLVLLMASLLSCFAVASPARASGTEATKAADYIVSTFK
                     ASGATRFGEVGSAADSLLALASTGDARYDAQIADILAVLQGEAAAFVKPDASGVSGAA
                     KLALAAVAAHADPRSFGGVDLVTTIEGGIGADGSFGAFPGPFSSGLAMVALSRSGASI
                     PDSMVDYLLTYAEPRTGTTGGGFGCAPYPFKKGSACSAADPDSTAMAVLGLQASGSTK
                     AKAAATDALAWLASTQQADGSWQNYSPVNSTGVVGPLFAAGSEQATRATAYVISKQLA
                     SGALSTGLDNKANLLATQQGIFALTDQTYATIGLSTATPTATAAPESTATAAPETSAP
                     ATPSGTDPAVMWTVGALLLLLLGGAAAASRSRR"
     gene            complement(6652..7275)
                     /locus_tag="PLF56_03765"
     CDS             complement(6652..7275)
                     /locus_tag="PLF56_03765"
                     /inference="COORDINATES: ab initio
                     prediction:GeneMarkS-2+"
                     /note="Derived by automated computational analysis using
                     gene prediction method: GeneMarkS-2+."
                     /codon_start=1
                     /transl_table=11
                     /product="hypothetical protein"
                     /protein_id="HOQ52713.1"
                     /translation="MADNDSGNILDDLVAKAKDLGEQVKEFAEDAFDNVKEFASDTVE
                     KGQHAFEDIKDRLDGDDDTPAADPAGDGVDPIGDPADPAGDPVDPAGTPDDPSAADTV
                     SSAHDVPVSDSVEDAADELSTSAEVGSLDDAEARGSSGEADPSPESLPGDDAGDLNTS
                     YGGDVGESGVNDFGSTDQPVDSFGDTEASALDAPPEGPDADTSKPLS"
     gene            complement(7338..>7698)
                     /locus_tag="PLF56_03770"
     CDS             complement(7338..>7698)
                     /locus_tag="PLF56_03770"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_011757299.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_function: GO:0008168 - methyltransferase activity
                     [Evidence IEA]"
                     /codon_start=2
                     /transl_table=11
                     /product="methyltransferase"
                     /protein_id="HOQ52714.1"
                     /translation="VNPRALTLTADNARAHRVGERVRTLQPDAVEPDARYDEIWSNPP
                     IRIGKAALHELLLHWLPKLKPCGVARLVVGRNLGADSLQRWLIDQGWSCERVGSAKGF
                     RVFAVRRALSGASTGDA"
BASE COUNT         1122 a         2588 c         2754 g         1234 t
ORIGIN      
        1 tcacagggcc gggcgaagcc agcaccacgc gcacctcgac gccgaagtcc ttgagctttt
       61 tctcgatcag gcgcgaggtc atctcgagcg tgtcggccga caccgtttcc tggcgcgcct
      121 gggcggcgtc gagccggcgc gggtcctgac cggctacgtc gatgcttccg gcgcaggtca
      181 gcgcgccctg cagcgcccac agcagcgacg acttgccggc cccgttgcga cccagcaggc
      241 tggtcacggt gccggcgacc aggtcgaggt ccaccgcgtt gacggccacc agttggccgt
      301 agcgaacctc gaggtcccgg gtgcggagcg cgaccggctc ggtgctcacc ggcacccggt
      361 cgggcggggt gggcagcacg tcgtcggacg ccacccggcg gcgcgcctca cgcacgctga
      421 gcggcacctc gggccagccc aacacccggg cgagctcggc cagcgggggc gtgatgctgg
      481 cgtgggccag cacttgggcc ggcgggccgt gcaccacccg tccgtcgccg ggcaggtaga
      541 gcatcgagtc ggcggcctgc atcacccgct ccagccggtg ttcggccagc accacggtca
      601 ggccgacatc gtgcaccagg gtgccgatgg cggccagcac gtcctgggct gcggtcgggt
      661 cgagcgccga ggtgggttcg tccagcacca gaatgcgggg ctgcgctgcc agcacggcgg
      721 cgatcgcgac gcgctgctgc tggccgccgg acaactccac cagcggacgt cggcgcaggt
      781 ccgcgatgcc catcaggtcg agcgtctcct cgacccgttt gcgcatcgcc acgggtgcga
      841 tgccgagctg ttccatgccg taggcgatct cgtcctcgac ggtgtcggtg acgaagcctc
      901 gcagcgggtc ctggccgacg tagcccacca cgtccgccag atcgcgtgga cggtgggtgg
      961 cggtgtcgcg gccgtccacc agcacccggc cggccagggt gccgccggtg aaatgtggaa
     1021 ccagtccgtt gatggcgccg agcagggtcg acttgcccga cccggtcggg ccgatcgcca
     1081 ggcacaggtc gccctcgcgc aactcgaagc tgacccggtc gagcaccgga gcccctgcgc
     1141 cggcgtaggt cacgctgacc cggtcgaaac tgatcaccgc catgtcggct ccggttcgtt
     1201 ggtgcggggc cgcagcaggc gcacccgggt gtcgttgcgg gtggattcga cggcggtcgc
     1261 gcgtcgttgc ctggtcaacg gcagcggtgc gagcaccagg gcgatcacca gcagcatcgg
     1321 tgcgctcacc gtcggccaca ccagcgggtt ggtggtgggg ctgagcgcga gcgggtcgag
     1381 tagcgcggcc agggccgggc tgagcgcgtc cgggtcgagc cagccgagcc cgagcacgat
     1441 caccgcgcac agcacacccg agagggcgac gagggtctcc gtgctgcgcc aggggtgcgg
     1501 acggtacgtg gtcaccttca gccgtgcgcc ggcggccttg agcccgatgc cggcgccgac
     1561 cataccgagc accatcaacc acagtgcgac ctgccagtac gtggagccga gcagcaggaa
     1621 gaccccgagg gtggccgcca tggacgacag cagcatcaac ggcagggtgc cccgggtggg
     1681 cagcccgctg gtgcgggcga agccacgtga ttccatgccg gccgcgagcg acagcgaccg
     1741 gtcgatggca tcggccagca cgggcatcgc gaccgtgccg agcgcacgca ggttggtggt
     1801 cgaggcgccg cgcaaccggc gggcccggcg cacccgttga atgctctcga tcaactgcgg
     1861 cgccacgctg agtgcgatca ccacggccgt ggacgcctcg tacagggcgg ccggcaccga
     1921 gcgcagcgcc tggcgcgggt tggccagtgc gttggccgca ccgatgcaga gcagcatcac
     1981 ggccagccgc agcgactcgt acaacgtggc gacgacggcc tcggcggtga ccactccgcc
     2041 cagttgaatg cccgcggccc agtccggtag cggaacggac ggcagggtga acagcacggt
     2101 ttcgccggcc gagccgccga gcagaatccg gaagaacaac cgcatgccga tcacgaacaa
     2161 cgccagcagc aggtagaccc gcaccgactt ggcccagggt gcgtcactgc gccgcagcat
     2221 caccaccacg accatcgcgg tggcgatcag cagcaggagc agcgggttgg tggtgccgtt
     2281 cacggcgatg ccgatgctga tcgcccagca ccaccaggcc cacggatgca gtgagttggt
     2341 cagggcttgc gtcccttcag cagccaccag ccgccgaggc ctccgccggc gagcacgacg
     2401 accgagccga cgacgatcgc gcccacgggg ctgccggcgt tgccgcttgc cgcggcggtg
     2461 gcgctgggtt cctgcgccgc gacgtcggtc ccgatgccgg acgggtcgtt gccgggggct
     2521 tcggtggtgg tggcagtggt tgtggaagca gcggaggcgg acgtcgctgc cgtcgccttc
     2581 gcggacgcct tgccggtcgc cgtcgcggtc gcggaggagg tcgcctttgc cgaggccgtc
     2641 cggctcgctg tcctggtggc cgagggagac gccttgggag tgttggttga cttcgaaggc
     2701 ttcggcgtcg gcttgctggt ggttttcgtc ggcgtcggac tcggcgaaga ggaggcggcg
     2761 ggcggcttgg cgcccgggcc actgttaccg ccgctgatcg agacgtagtg ccagccttcg
     2821 gcggtgcccc gcttgggctt gaagccgccc gcgcccaggt tggagtagct ccacgacgac
     2881 cagtccccgt cggactgttg cgtggcatgc cagtacgacc agtaggcctt gttgatgtcg
     2941 ggcttggacg gcttgccgtt caacttgaag acgaagccgt cctcgaccac gggggcgaag
     3001 ccggcgctgc gcagcgcggc caacccggtg gcgtagctgg tggcgcatcc ggtgctggtg
     3061 ccgccgccga gcgaaccgaa atcgaccacc acccagacgc cgctgcatga ggcggcctgc
     3121 ggcagcgacg gtgcctgcgg caccgaggcc tgctgggcgg tggactgtgc cgcgctggtc
     3181 tgcgcggcgc cgaccgaggc gggcgcacag gcgctgacgg cgaaggccgc cagcgccagt
     3241 gcgaggaacc tcttcttcac ttggtgacct tcgcggttgc cgtggacgtg cgcgacaccg
     3301 tctgcagacc gctgccgctg gccgtcacct tgaccgtgat cgtcttgccc acgtcggact
     3361 tggtcagctt gtaggtcgcc ttggtggcct tcgaaatcgc cttgcccgag cgataccact
     3421 gcaggacgac cttggtgggc gtaggcgtcc aggtgccggg ggtcgcggtc agggtctggc
     3481 ccaccttcgt cttgccggcg atggtgacgt tcccggtgct gctgtagccc ttgggcggcc
     3541 gggtgaacgc ggtcgtggtc gcgtcgccgg tgtacacctt ccaataccag ccttcagcag
     3601 cgcccttggc aggcttcgag tccgcagccc ccagttggta ttcctgccat tcgccccagc
     3661 tgccgtcagc cttcgggttg gcgtaccagt aactccagta cttgctcgtg ggcgtccagg
     3721 gaacgagggt ctgcgggaag ccgttgatgc gagtgagaag gccggacttg tccttggcga
     3781 acgagaaacc ggcactcttc agagcgtcca gcccgttgcc gaacttggtc gcgcaacgca
     3841 cggtgtcctg cccgttgccg cgatccacca gcacccacac gccggtgcac gacgtggtcg
     3901 ccttgggcag cgacggcggg ttcttcgggc acttacccag gttgaacgac acgtccagca
     3961 gggacgtgcc ggtgatcagg tacaacgcgt tggcggtggc catcacgttg gagtcggtac
     4021 ccgcgtccag cgcgttcgga aagccgccgt ccgcgttctg cacgcctccg agccaagcct
     4081 tagccttggc tacggcggcg tcctgaccac cggtggcctt gagcgccgag gcgaccagtc
     4141 cggtcgagtc gacgggggag taggcctccc agtagccctc ggaggtctgg tggtccgtgg
     4201 cccatgtggt ggccgagctg agctgtggct tgagttggcc gatcgccttc agcgccaggg
     4261 tgcccatggc tgtggtgtcg gggtcggcgt tgaaggtgcc ggccgggaac tcataaccga
     4321 aggcgccgtt gtcgccctgc tggctgagca gattggtcag cagcgtgacg ggaatggtgg
     4381 ccttggcccg cttcagcgcg atgaggccga ggctctggct gaaggccgac ttagaaccgc
     4441 cgatctgccc gtcggcgggc aggctcttgg tgatcagcga gaccaggttg tacccggcga
     4501 acttcttcgg atcgagcccg agcgaggtca ccgtgatcgc caggttggcc gcgcgcgcct
     4561 ggttgagctt cttgcccgag tagaggtagc tcttcgcgtt cttctcgatc tgcttcacca
     4621 gggtgcgcgt cgccggcgcg taggtgcaat cgttcgtggt ggccaggccg agggccgcgg
     4681 tcatcgacgc tgcagcgccg tcgctgctcg tcggcaggtt cttgaccagg aagttcgcgg
     4741 ccttggtggc gttgggttcg gcggcctgcg cgtgcggggc cgacagggtg agcgtagaga
     4801 ctgcgagtgc ggctgctgcc ccgacagccg tcacccgctt gagcactggg tgcatctgga
     4861 tcctttggtc gtgcgtgcca ctcgaagggc tccagaccag atgtcgggct cccccgcgta
     4921 ccacgacggg ttggtgatga gccggatccg ggcaggcatt ccgacttcgc cattgctggc
     4981 gtcacggctg cgggtcagtc ccggcttctc accggattcc cctgcacgat tcaggtgatc
     5041 gacacgatag cccaaccgca ccccttgtgt cgcaggggtc gaggatcagc gacgactgcg
     5101 cgaggcggcc gcagcgccgc cgaggagcag cagcagtagt gcgcccaccg tccacatgac
     5161 agccggatcg gtaccggacg gcgtggccgg agcggaggtt tccggggccg ccgtcgcggt
     5221 cgactcaggc gccgcggtcg ctgtcggtgt cgcggtgctc agcccgatcg ttgcgtaggt
     5281 ctggtccgtc aacgcgaaga tgccctgctg ggtggcgagc aggttcgcct tgttgtccag
     5341 acccgtgctg agtgccccgg aggcgagctg tttcgagatc acgtaggccg tggccctggt
     5401 ggcctgctcg gagccggccg cgaacagcgg gcccaccacc ccggtggagt tcaccggtga
     5461 gtagttctgc cacgagccgt ccgcctgctg ggtcgaggcg agccaggcca gggcgtcggt
     5521 cgccgcggcc ttcgccttgg tcgagccgga ggcctgcagg cccaacaccg ccatcgccgt
     5581 gctgtcggga tcggccgcgg agcacgcact gcccttcttg aacgggtagg gggcacagcc
     5641 gaagccgcca cccgtggtgc cggtgcgcgg ctcggcgtaa gtgagcagat aatcgaccat
     5701 cgagtcggga atggacgcgc cgctgcggct gagcgccacc atcgcaagcc cggagctgaa
     5761 ggggccggga aaggcgccga acgagccgtc cgcgccgatg ccaccctcga tggtggtgac
     5821 caggtccacg ccgccgaagc ttctcgggtc ggcgtgcgcg gcgaccgcgg ccagggcgag
     5881 cttggcggcc cccgacaccc cggacgcgtc cggcttcacg aacgcggccg cctcgccctg
     5941 caggacggcg aggatatcgg cgatctgagc gtcgtagcgg gcatcgccgg tcgaggcgag
     6001 ggcgagcagc gaatcggcgg ccgaaccgac ctctccgaag cgtgtcgccc cgctggcctt
     6061 gaaggtcgac acgatgtagt cggctgcctt ggtggcctcg gttccgctcg cgcgcgcggg
     6121 cgaagcgacc gcgaaacagc tcagcaggga tgccatcagt agcaccagga cgcgtctgat
     6181 cagggccatg gcgagggtcc tttcgaaggg acctaccacg ctagccgacg cgagccgggc
     6241 cgtggggaag tgggcacgca cccacagacc gcgtccgaga accatcgagc cggaaacgcc
     6301 cggatgcacc ggtgatcacc gaaacgggaa ccgggcgatc ggctcgcagt tcgacgcttt
     6361 catggcgcac aactgcgccg gcctgcgggc aaccacccgg ttcgccctcg cgcaactggc
     6421 cgccacccgt gcgggaagca ttgatccatc gctggtcgag cgtgtcgtac cggtgcgatg
     6481 aggggctttc gacacgctcg acccgcgggt cacgtggtga tcagcgcatc ttcagctgcc
     6541 gctcgccgcg gcagtcatcg gatcagggct cagcccgtcg gcggcagcgg gtcttcaaca
     6601 cgcctggtcg ggcggggtgc cggcccactc gaccaggtga gtggcgatgc ttcagctgag
     6661 cggtttgctg gtgtcggcat cgggaccctc gggcggggcg tccaacgcgc tggcctcggt
     6721 gtcgccgaag ctgtccaccg gttggtcggt gctgccgaag tcgttgacac ccgactcgcc
     6781 gacatcgccg ccgtaggaag tgttgaggtc gccggcatcg tcgcccggca acgactcggg
     6841 gctcggatcg gcttcgccac tgctgccgcg agcctcggcg tcatcgagcg acccgacctc
     6901 ggccgaggtg gaaagctcat cggcggcgtc ttcgaccgag tcgctcaccg gcacgtcgtg
     6961 ggcgctgctc acggtgtccg cggcgctggg atcatccggt gtgccagccg ggtccaccgg
     7021 gtcgccggcc gggtccgccg ggtcgccgat cgggtccacc ccgtcgccgg ccgggtccgc
     7081 tgccggggtg tcgtcgtcac catcgagacg gtccttgatg tcctcgaatg cgtgctggcc
     7141 cttctcaacg gtgtcggagg cgaattcctt cacgttgtcg aacgcatcct cagcgaactc
     7201 cttcacctgc tcgcccaggt ctttcgcctt ggcgaccagg tcgtcaagaa tgttgccgct
     7261 gtcgttgtca gccacgccga actccttcga actgtcgggt cgggccatcc gccctgtgcc
     7321 gccaacagta ggcaggttca ggcatcaccg gtagaggcgc cggacagtgc tcgtcgaacc
     7381 gcgaacactc gaaaaccctt cgcgcttccg acgcgctcac acgaccagcc ttggtcgatc
     7441 agccagcgct gcaacgaatc ggcccccagg ttgcggccga ccaccaagcg ggccactccg
     7501 cagggcttga gcttcggtag ccagtgcagc agtaactcgt gcaacgccgc cttgccgatc
     7561 cggatcggcg ggttggacca gatctcgtcg tagcgcgcgt ccggttcgac cgcgtccggt
     7621 tgaagggtgc gtacccgctc accgacccgg tgggcccggg cgttgtcggc agtcagtgtg
     7681 agcgcgcgcg ggttgacg
//
LOCUS       DAOSUG010000074         7667 bp    DNA     linear   ENV 10-JUN-2024
DEFINITION  MAG TPA_asm: Micropruina sp. isolate AMR_MDS_2195
            NODE_4824_length_7667_cov_4.610352, whole genome shotgun sequence.
ACCESSION   DAOSUG010000074 DAOSUG010000000
VERSION     DAOSUG010000074.1
DBLINK      BioProject: PRJNA925477
            BioSample: SAMN32864262
            Sequence Read Archive: SRR4236673
KEYWORDS    WGS; ENV; Metagenome Assembled Genome; MAG; Third Party Data; TPA;
            TPA:assembly.
SOURCE      Micropruina sp. (wastewater metagenome)
  ORGANISM  Micropruina sp.
            Bacteria; Bacillati; Actinomycetota; Actinomycetes;
            Propionibacteriales; Nocardioidaceae; Micropruina.
REFERENCE   1  (bases 1 to 7667)
  AUTHORS   Abdulkadir,N., Saraiva,J.P., Zhang,J., Stolte,S., Gillor,O.,
            Harms,H. and Rocha,U.
  TITLE     Genome-centric analyses of 165 metagenomes show that mobile genetic
            elements are crucial for the transmission of antimicrobial
            resistance genes to pathogens in activated sludge and wastewater
  JOURNAL   Microbiol Spectr 12 (3), e0291823 (2024)
   PUBMED   38289113
REFERENCE   2  (bases 1 to 7667)
  AUTHORS   Abdulkadir,N.
  TITLE     Direct Submission
  JOURNAL   Submitted (24-MAR-2023) Environmental Microbiology, Helmholtz
            Center for Environmental Research -UFZ, Permoserstr 15, Leipzig
            04318, Germany
COMMENT     The annotation was added by the NCBI Prokaryotic Genome Annotation
            Pipeline (PGAP). Information about PGAP can be found here:
            https://www.ncbi.nlm.nih.gov/genome/annotation_prok/
            
            ##Genome-Assembly-Data-START##
            Assembly Method        :: SPAdes v. 3.11.1
            Genome Representation  :: Full
            Expected Final Version :: Yes
            Genome Coverage        :: Not Applicable
            Sequencing Technology  :: Illumina MiSeq
            ##Genome-Assembly-Data-END##
            
            ##Genome-Annotation-Data-START##
            Annotation Provider               :: NCBI
            Annotation Date                   :: 05/04/2023 04:57:10
            Annotation Pipeline               :: NCBI Prokaryotic Genome
                                                 Annotation Pipeline (PGAP)
            Annotation Method                 :: Best-placed reference protein
                                                 set; GeneMarkS-2+
            Annotation Software revision      :: 6.5
            Features Annotated                :: Gene; CDS; rRNA; tRNA; ncRNA
            Genes (total)                     :: 2,776
            CDSs (total)                      :: 2,750
            Genes (coding)                    :: 2,714
            CDSs (with protein)               :: 2,714
            Genes (RNA)                       :: 26
            tRNAs                             :: 24
            ncRNAs                            :: 2
            Pseudo Genes (total)              :: 36
            CDSs (without protein)            :: 36
            Pseudo Genes (ambiguous residues) :: 0 of 36
            Pseudo Genes (frameshifted)       :: 2 of 36
            Pseudo Genes (incomplete)         :: 32 of 36
            Pseudo Genes (internal stop)      :: 2 of 36
            ##Genome-Annotation-Data-END##
FEATURES             Location/Qualifiers
     source          1..7667
                     /organism="Micropruina sp."
                     /mol_type="genomic DNA"
                     /submitter_seqid="NODE_4824_length_7667_cov_4.610352"
                     /isolate="AMR_MDS_2195"
                     /isolation_source="Activated sludge of municipal
                     wastewater treatment plants"
                     /db_xref="taxon:2737536"
                     /environmental_sample
                     /geo_loc_name="Canada"
                     /lat_lon="49.25 N 123.1 W"
                     /collection_date="2015-06-16"
                     /metagenome_source="wastewater metagenome"
                     /note="metagenomic"
     gene            complement(<1..203)
                     /locus_tag="PLF56_03775"
     CDS             complement(<1..203)
                     /locus_tag="PLF56_03775"
                     /inference="COORDINATES: ab initio
                     prediction:GeneMarkS-2+"
                     /note="Derived by automated computational analysis using
                     gene prediction method: GeneMarkS-2+."
                     /codon_start=1
                     /transl_table=11
                     /product="hypothetical protein"
                     /protein_id="HOQ52715.1"
                     /translation="MEASSGEIDSAASGVVRWPVRVETGHYAHPVAPEVYDCVKFLLA
                     REGSGTITGDWGERRFRMGDLMLV"
     gene            complement(245..2788)
                     /gene="arsA"
                     /locus_tag="PLF56_03780"
     CDS             complement(245..2788)
                     /gene="arsA"
                     /locus_tag="PLF56_03780"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_016831302.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_function: GO:0005524 - ATP binding [Evidence IEA];
                     GO_function: GO:0015446 - ATPase-coupled arsenite
                     transmembrane transporter activity [Evidence IEA];
                     GO_function: GO:0016887 - ATP hydrolysis activity
                     [Evidence IEA];
                     GO_process: GO:0071722 - detoxification of
                     arsenic-containing substance [Evidence IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="arsenical pump-driving ATPase"
                     /protein_id="HOQ52716.1"
                     /translation="MIELLHNPPRFLFLTGKGGVGKTSIACAAAVDLARRGNRVLLVS
                     TDPASNVGQVFDQRVGYAITPIVGVTGLDALEIDPEAAAEEYRGRALAPIRDFLSVKD
                     LADVTEQLSGSCTTEIASFNEFTAFLAGNDIARGYDHIVFDTAPTGHTVRLLKLPGEW
                     TSFLEDGLGDASCLGPMAGLDKTRTTYADALTALADPSVTRLGLVARPQTSSLAEASR
                     TADELAEAGVHATHLVINGILPSEPISDVLADAIRAEETAAIAAMPVNLAGLAIDRLP
                     LRSRDVMGLDALTTLLSEAPDHTPALAAPRANAPAGSSDSLAATIEQLAAKDHGLIMV
                     VGKGGVGKTTMAAAIAVGLSDRGKDVLLTTTDPAAHLAWTVGEAAPFEVSSIDPVQAV
                     ADYRRHVMNTKGAGLDADGRANLAEDLRSPCTEEVAVFQAFAAAVEQSAHRFVVMDTA
                     PTGHTLLLMDATGSYHREVVRNLPEQAGLTPLARLQDPDNTSVIVVTLPETTPVLEAT
                     GLAADLTRANITTWGWIVNRSLTPTDTTSPLLAERINAEQTPLREAQAQTGRVAVVPY
                     SDTPPVGVNQLRQLSTASDRDAGHAVREQVRLRYAQAATAVRAGAHNTDLLADSTCCS
                     PASCCGNPGADIGGFGAGLYDATEAAELPLEAIEASLGCGNPTTVAELCEGERVLDLG
                     SGGGIDVLLSARRVGQSGFAYGVDMTDEMLDLARTNAANAGATNVEFLKGTIEDVPLP
                     DGAVDVVISNCVINLSTDKPAALAEMFRVLAPGGRIGISDVVAEDHLTPAERAERGSY
                     VGCIAGALSKSEYLQGLAAAGFTDPTVEFTHQVADDMHSAIIRATKLVR"
     gene            complement(2801..3247)
                     /gene="arsD"
                     /locus_tag="PLF56_03785"
     CDS             complement(2801..3247)
                     /gene="arsD"
                     /locus_tag="PLF56_03785"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_004809632.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_function: GO:0003677 - DNA binding [Evidence IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="arsenite efflux transporter metallochaperone
                     ArsD"
                     /protein_id="HOQ52717.1"
                     /translation="MIQVFEGPLCCNTGVCGPDPDQALVDFTADLDWLTRQGVSVRRA
                     NLAQDPAAFADSAVARAFVQKVGASGLPLIVADDVTVATGRYPSRAELARIAGVEAPA
                     ENTATGCCGSATPTPAASVCCGDEAPVSSGCCGSDQSVTIQSGSQA"
     gene            3337..3711
                     /locus_tag="PLF56_03790"
     CDS             3337..3711
                     /locus_tag="PLF56_03790"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_009396705.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_function: GO:0003677 - DNA binding [Evidence IEA];
                     GO_function: GO:0003700 - DNA-binding transcription factor
                     activity [Evidence IEA];
                     GO_process: GO:0006355 - regulation of DNA-templated
                     transcription [Evidence IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="metalloregulator ArsR/SmtB family transcription
                     factor"
                     /protein_id="HOQ52718.1"
                     /translation="MVQHLPIIDCAPQGASALADEEAERVAATFKALSDPVRVKLLHH
                     ISANCCSSVCACHMPDSLGISQPTLSYHLARLVKAGLISREMRHKWAHYHATPQGLAT
                     VRAFLAGLPDPDNCSADPSITC"
     gene            3856..4833
                     /gene="trxB"
                     /locus_tag="PLF56_03795"
     CDS             3856..4833
                     /gene="trxB"
                     /locus_tag="PLF56_03795"
                     /EC_number="1.8.1.9"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_013864593.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_function: GO:0004791 - thioredoxin-disulfide reductase
                     activity [Evidence IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="thioredoxin-disulfide reductase"
                     /protein_id="HOQ52719.1"
                     /translation="MSMQDVELVIVGSGPAGYTAAIYAARAGLAPVVLAGSVTAGGAL
                     MTTTEVENFPGFTDGIQGPELMEAMRAQAERFGARIIYDDATCLALTESTKVIETSSS
                     GTFQARAVILAMGSAYRKLGLPEEARLSGHGLSWCATCDGFFFRDREIAVIGGGDSAM
                     EEALFLTRFAAKVTLVHRRGEFRASRIMAERALADPKIEVAWNSEVAAILGEANVTGL
                     RLRDTVTGTERVLDVAGTFVAIGHDPRSELVADQVSTDAEGYVIVAHPTTRTNVAGVF
                     AAGDLVDHNYRQAITAAGTGCASALDAQHYLSTLDLPTQIESPTLEVSA"
     gene            4830..5153
                     /gene="trxA"
                     /locus_tag="PLF56_03800"
     CDS             4830..5153
                     /gene="trxA"
                     /locus_tag="PLF56_03800"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_013864594.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_component: GO:0005737 - cytoplasm [Evidence IEA];
                     GO_function: GO:0015036 - disulfide oxidoreductase
                     activity [Evidence IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="thioredoxin"
                     /protein_id="HOQ52720.1"
                     /translation="MSITPVTDATFVSEVLESELPVVVDIWAAWCGPCKAIAPILEHL
                     AAEYAGRVKIVKIDADANPETVTTTGVTSIPTLSFYRDGERVDMLIGAHPKPSIAAKI
                     DGLLA"
     gene            5150..6265
                     /gene="arsB"
                     /locus_tag="PLF56_03805"
     CDS             5150..6265
                     /gene="arsB"
                     /locus_tag="PLF56_03805"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_020385339.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_function: GO:0008490 - arsenite secondary active
                     transmembrane transporter activity [Evidence IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="ACR3 family arsenite efflux transporter"
                     /protein_id="HOQ52721.1"
                     /translation="MTATATAPTRATPKRLSALDRWLPLWIGLAMIAGLLLGRLVPAV
                     SDVLAHLEVGGISVPIGLGLLVMMYPVLAKVRYDKIAAVTGDKRLLVSSLVLNWILGP
                     AVMFALAWLFLPDLPEYRTGLIIVGLARCIAMVVIWNDLACGDREATAVLVAINSIFQ
                     VVAFSLLGWFYLTVLPGWLGLDTQGLEVSVGQIALNVLVFLGVPLIAGFASRWIGEKR
                     RGRDWYENRFLPRVGPWALYGLLFTIVLLFALQGEAITSSPFDVARIALPLLVYFALM
                     WFVGLLLGKGLGLGYARSTTLAFTAAGNNFELAIAVAIGTFGAASGQALAGVVGPLIE
                     VPVLVGLVYVSLWAARAWFHTDPYAPDTATTDLRRTP"
     gene            6262..7254
                     /locus_tag="PLF56_03810"
     CDS             6262..7254
                     /locus_tag="PLF56_03810"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_017883888.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_function: GO:0003700 - DNA-binding transcription factor
                     activity [Evidence IEA];
                     GO_process: GO:0006355 - regulation of DNA-templated
                     transcription [Evidence IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="metalloregulator ArsR/SmtB family transcription
                     factor"
                     /protein_id="HOQ52722.1"
                     /translation="MTETPTTVETCAPAEAHAIGLDAATAVANTLKALADPLRLRMLS
                     AIATDPRGESCVCDLAELADVSQPTVSHHLKVLKDTGLLSSQRRGTWVYYRLVPTRQA
                     AVTALLDGFASTVAADPAEDRPESPREVDSHVIDRLAEELADEYDTLSRDLVVTMVRE
                     SYAGLLRSAKIARHLVPLTERFARQRLTDLTRDRETGVPQVLFVCVQNAGRSQLAAAL
                     VNQMADGKVVARSAGSRPAPDVHPHVRSLLTQIDGEDAATARFPKPLTDDAVRAADVV
                     ITMGCGDVCPIIPGVRYEDWAVGDPALASVEGVEAIRDDIAARVRTLLDSLTSR"
     gene            7271..>7667
                     /locus_tag="PLF56_03815"
     CDS             7271..>7667
                     /locus_tag="PLF56_03815"
                     /EC_number="1.20.4.4"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_007078856.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_function: GO:0030612 - arsenate reductase (thioredoxin)
                     activity [Evidence IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="arsenate reductase ArsC"
                     /protein_id="HOQ52723.1"
                     /translation="MTDAKPSVLFVCIHNAGRSQMAAGYLRHLAGDRIEVRSAGSMPA
                     DQINPIAVEAMREEGIDITAEQPKVLTPEAVQASDVVITMGCGDACPYYPGKRYEDWK
                     LDDPAGQGIDAVRPIRDDIRARIEGLIASL"
BASE COUNT         1199 a         2635 c         2564 g         1269 t
ORIGIN      
        1 accagcatca gatcgcccat gcggaaccga cgctcgcccc agtcaccggt gatagtccct
       61 gagccctcgc gggctagcag gaacttcaca cagtcatata cctccggcgc tacagggtgc
      121 gcgtagtggc cggtctcgac gcgcaccggc caccgaacca cgccgctcgc tgcgctgtcg
      181 atctcaccgc tgcttgcctc catggctcat ccccagcgag ctcactcttc gaaggttccg
      241 gaagctaacg caccaacttc gtcgcgcgga tgatcgctga gtgcatgtca tcggcgactt
      301 ggtgggtgaa ctcgacggta gggtcggtga atccggctgc ggcgaggccc tgaaggtatt
      361 cactcttgga gagcgcgccg gcgatgcacc ccacgtatga accgcgctcg gctcgttcgg
      421 ccggggtgag atggtcttcg gcgacgacat cggagatgcc gattcttccg ccgggagcga
      481 gcacacggaa catctccgcc agggcggccg gcttgtctgt cgacaggttg atgacgcagt
      541 tggagatcac gacgtcgact gcgccgtcgg gtaggggtac atcctcaata gtgcctttga
      601 ggaactcgac gttggtcgct cccgcgttcg cggcgttggt gcgtgccagg tcgagcatct
      661 cgtcggtcat gtcaacgccg taggcgaatc cggactggcc gacacggcgt gcggagagca
      721 ggacatcgat tccgcccccg gaaccgaggt ctaggacgcg ctcgccctcg cacagctctg
      781 cgaccgtggt tgggttgcca catcccagac tcgcctcgat cgcctccagc ggcaactcgg
      841 cagcctccgt cgcgtcgtag agcccggccc cgaagccacc gatgtcagcg ccgggattgc
      901 cgcagcagga agcgggtgag cagcaggtac tgtccgccag caggtcggtg ttgtgagcgc
      961 cggcgcggac tgccgttgcg gcttgcgcgt agcggaggcg tacctgctca cggacagcgt
     1021 gaccggcgtc ccggtccgag gcggtggaga gctggcgaag ttggttcaca cccacgggag
     1081 gggtgtcgct gtagggcacg acggcgaccc ggccggtctg cgcctgggcc tctcgcagag
     1141 gtgtttgttc ggcgttgatc cgttcggcca gcagggggct ggtggtgtcg gtcggggtga
     1201 gggagcggtt gacgatccat ccccaggtgg tgatgttggc tcgggtgagg tcggcggcca
     1261 ggccggtggc ctcgaggacg ggggtggtct cgggcagtgt gacgacgatg acgctggtgt
     1321 tgtcggggtc ctggagtcgg gccagcggcg tgagcccggc ctgctcgggc aggttgcgga
     1381 cgacctcgcg gtggtaggag ccggtggcgt ccatgaggag cagggtgtgt ccggtggggg
     1441 cggtgtccat gaccacgaac cggtgggcgg attgctcgac ggcggcggcg aaggcctgga
     1501 agacggccac ctcttcggtg cagggcgagc gcaagtcctc ggcgaggttg gcccgaccgt
     1561 cggcgtccag gcccgcgccc ttggtgttca tgacgtggcg cctatagtcg gccacggcct
     1621 ggacgggatc gatactggag acctcgaagg gcgccgcttc gccgaccgtc caggcgagat
     1681 gcgcggccgg gtcggtggtg gtcagcagca cgtccttgcc gcggtcggac aaaccgacgg
     1741 cgatcgccgc agccatcgtc gtcttcccga cgccgccttt ccccacgacc ataatcaatc
     1801 cgtggtcctt ggccgccagc tgctcgatcg tcgcggccag agaatccgac gagccggcgg
     1861 gggcgttggc gcgcggtgcg gctaacgccg gggtgtggtc cggggcctcg gagagcagcg
     1921 tggtgagggc gtcgaggccc atcacgtcgc gggagcgcag cgggagccgg tcgatcgcca
     1981 gcccggcgag gttgacgggc atggcggcga tggcggcggt ctcctcggcg cggatcgcgt
     2041 cggccaggac atcggagatg ggttcgctgg ggaggatgcc gttgatgacc aggtgggtgg
     2101 cgtggacgcc tgcttcggcg agttcgtcgg cggtgcggga cgcctcggcc agcgatgagg
     2161 tttgtgggcg ggcaaccagg cccagtctcg tcacggacgg gtcggccagg gcggtgagcg
     2221 cgtcggcgta ggtggtgcgg gtcttgtcga ggccggccat cggaccgagg caggacgcat
     2281 cgccgagccc gtcttcgagg aaggaggtcc attcgccggg gagcttgagc agccggacgg
     2341 tgtggccggt cggggcggtg tcgaagacga tgtggtcgta gcctcgcgcg atgtcgttgc
     2401 cggccaggaa ggcggtgaac tcgttgaacg aggcgatctc ggtggtacag gacccggaga
     2461 gctgctcggt gacgtcggcg aggtccttga ccgagaggaa gtcccggatc ggggcgaggg
     2521 cgcgccctcg gtactcctcg gcggcggcct cggggtcgat ctccagcgcg tcgagcccgg
     2581 taacgccgac aatcggggtg attgcgtacc cgacccgctg gtcgaagacc tggccgacat
     2641 tcgaggccgg gtcggtggag accagcagga cccggttgcc ccggcgggcg aggtcgaccg
     2701 cggcggcgca ggcgatgctc gtcttcccga caccgccctt cccggtcagg aacaggaacc
     2761 tcggcgggtt gtgcagcagc tcgatcatct ccgatcagcc ctacgcctga gagccggact
     2821 ggatggtcac cgactggtcc gaaccgcagc atcccgacga caccggggcc tcgtcgccac
     2881 agcacaccga cgctgccggg gtgggcgtgg cgctaccgca gcacccggtc gccgtgtttt
     2941 cggcgggcgc ctcgacgccg gcgatccggg ccagctcggc gcgggagggg tagcgtccgg
     3001 tggcaaccgt cacgtcgtcg gccacgatca gcggcagccc ggacgcgccg accttctgca
     3061 cgaacgcccg cgcgacggcg ctgtcagcga acgcggcggg gtcctgggcc aggttcgccc
     3121 ggcgcaccga cacgccttgc cgggtaagcc agtcgaggtc ggcggtgaag tcgaccagcg
     3181 cctggtcggg gtcggggcca cacaccccgg tgttgcagca cagcgggcct tcgaagacct
     3241 ggatcatgag gacgcctctc tcggattgat gttcgtcaat atgaaggcta gggctcatat
     3301 cgacgtctgt caatctgagg tggtaagctg gcgcgcatgg ttcagcacct cccgatcatc
     3361 gactgcgccc cacagggcgc gtcggcgctc gccgacgagg aggccgagcg ggtcgccgcc
     3421 accttcaagg cactctccga tccggtccgg gtcaagctgc tgcatcacat ctcggcgaac
     3481 tgctgctcca gcgtgtgcgc ctgtcacatg cccgacagtc ttggcatcag ccagccaacc
     3541 ctctcctacc atctggcccg gctggtcaag gccgggctca tcagccggga gatgcgccac
     3601 aagtgggcgc actaccacgc cacgccgcag ggcctggcga ccgtgcgagc gttcctcgcc
     3661 ggactgccgg acccggacaa ctgctcggcg gacccgtcga tcacctgctg acccaccagg
     3721 cccacgacaa ccctcccgac cgactccgcc tcgaaagccg cgacgccgcg ggggccagcc
     3781 tgcccgtggc gtccgaacag cacccgccgc cgctcccggg ttgcatcttg cttcgatctc
     3841 cttcaatata gatacatgtc gatgcaagat gtggaactag tgatcgtcgg gtccggccca
     3901 gccggataca ccgcagccat ctacgccgcg cgagcaggcc tcgcgccggt cgtcctggct
     3961 ggatcggtga cggccggcgg agcgctgatg acgaccacgg aggtcgaaaa cttccccggc
     4021 ttcaccgacg gcatccaagg ccccgagctg atggaagcga tgcgcgccca ggcagagcgc
     4081 ttcggcgccc gcatcatcta tgacgacgcg acctgtcttg cactcaccga atccacgaag
     4141 gtcatcgaga ccagcagcag cggaaccttc caggcgcgcg ccgtgattct ggcgatgggg
     4201 tccgcatatc gcaagctcgg tctccctgag gaagcgcgac tctcgggaca tggcctgtca
     4261 tggtgcgcga cctgcgacgg cttcttcttc cgtgaccggg aaatcgcggt catcggcggt
     4321 ggcgattccg caatggaaga agcactgttc ttgacgcgct tcgctgccaa ggtcacgctc
     4381 gtccaccggc gcggcgagtt ccgggcgtcc cggatcatgg ccgagcgggc attggccgac
     4441 cccaagatcg aggtcgcatg gaacagcgag gtggccgcga tcctcggcga ggcgaacgtc
     4501 accggtctgc gcctgcgcga cacggtcaca ggcaccgaaa gagtgctgga tgtggcgggc
     4561 actttcgtcg caatcgggca cgacccgcgc tccgagcttg tcgccgacca ggtgtccacc
     4621 gacgccgagg ggtacgtcat cgtggcccat ccaacgacac gcaccaacgt ggccggcgtg
     4681 ttcgccgccg gtgacctggt ggatcacaac taccggcagg cgatcacggc cgctggcacc
     4741 ggctgcgcct ctgccttgga cgcgcagcac tatctgtcga ctctcgatct gcccacccag
     4801 atcgagagtc ccactctgga ggtttccgca tgagcatcac ccccgtcacc gacgccacgt
     4861 ttgtcagcga agtgctcgaa tcagagcttc cggtcgtagt tgatatctgg gctgcgtggt
     4921 gcggcccatg caaggccatc gctccgatcc tggagcactt ggccgccgag tatgcgggcc
     4981 gggtcaagat cgtcaagatc gacgcggacg ccaatcccga gaccgtgacc acaactggtg
     5041 tcacctcgat ccccactctt tccttctacc gcgacggcga gcgggtcgac atgctgatcg
     5101 gcgcgcaccc aaagccgtcc atcgccgcga agatcgacgg actcctggca tgaccgcgac
     5161 cgcaacggcg cccacacgcg ccactccgaa gcgcctgtcc gccctcgacc gttggctgcc
     5221 gctgtggatc ggactggcga tgatcgcagg cctcctactc ggtcgtctcg tccccgccgt
     5281 ctccgatgtg ctcgctcacc tggaggtggg aggcatctcg gtaccgatcg ggctggggct
     5341 gctcgtgatg atgtaccccg tgctcgcaaa ggtccgctac gacaagatcg cggccgtcac
     5401 gggcgacaag cgcctcctcg tctcatccct ggtgctgaac tggatcctcg gcccggcggt
     5461 catgttcgcc ctcgcctggc tgttcctgcc tgatctgccg gagtaccgaa cggggctcat
     5521 catcgtggga ctggcccgct gcatcgcgat ggtcgtgatc tggaacgacc tggcctgcgg
     5581 cgatcgagag gccaccgccg tgctggttgc gatcaactcc atcttccagg tcgtcgcgtt
     5641 ctcgctgctc ggctggttct acctcaccgt actgcccggc tggctcggcc tcgataccca
     5701 aggtctggaa gtgtcagtgg ggcagatcgc gctgaacgtc ctggtcttcc tcggcgtccc
     5761 gctcatcgcc gggttcgcct cgcggtggat cggcgagaag cgccggggac gcgactggta
     5821 cgagaaccga ttcctcccca gagtggggcc gtgggcgctc tatgggttgc tgttcaccat
     5881 cgtgctgctc ttcgcactcc aaggcgaagc gatcacatcc agtccgttcg atgtcgcccg
     5941 gatcgccctg ccgctactgg tctacttcgc gctgatgtgg ttcgtcgggc tcttgctcgg
     6001 caagggtctc ggactgggct atgcccgctc caccacactg gcgttcacgg cggcgggcaa
     6061 caacttcgaa ctcgccatcg cggtcgcgat cggcaccttc ggcgcggcat ctggacaggc
     6121 cctcgcaggg gtcgtcggcc cgcttatcga agttcccgtg ctcgtcggcc tcgtctacgt
     6181 ctcgctgtgg gccgcacgcg cctggttcca caccgacccc tacgctccgg acaccgctac
     6241 gaccgacctg aggaggacac catgaccgaa acacccacaa cggtcgagac gtgcgcaccc
     6301 gccgaggcgc acgcgatcgg cctcgatgcg gccaccgccg tggccaacac gttgaaggcg
     6361 ctggccgatc cgctccggct ccggatgctg tcggccatcg ccaccgaccc gcgcggcgag
     6421 tcctgcgtct gcgacctcgc cgaactcgcc gacgtctccc aacccaccgt ctcccaccac
     6481 ctgaaggtcc tgaaggacac cggcctgctc agctcccaac ggcgcggcac ctgggtgtac
     6541 taccgcctcg tccccacccg acaggcggcc gtcaccgccc tcctggacgg cttcgcatcc
     6601 acggtcgccg ccgatcctgc agaggatcgg cccgagtcgc cgcgtgaggt cgactcgcac
     6661 gtcatcgatc gcctcgccga agaactggcc gacgaatacg acacgctgag tcgcgacctg
     6721 gtggtcacaa tggtgcgaga gtcctacgcc ggcctcctcc ggtccgcgaa gatcgcccga
     6781 caccttgtac cactcaccga gcgcttcgcc cgccagcgcc tcaccgacct cacccgcgac
     6841 cgcgagacgg gtgtgccgca ggtgttgttc gtgtgcgtac agaacgccgg ccgctcccag
     6901 ctcgcggccg cgctggtcaa ccagatggcc gatggcaagg tcgtcgcccg ctccgccgga
     6961 tcacgaccgg caccagatgt ccacccgcac gtgcgctcgc tgctcaccca gatcgacggc
     7021 gaggacgccg ccaccgcgcg gttccccaag ccccttaccg acgacgcggt ccgcgccgcc
     7081 gatgtcgtca tcaccatggg ctgcggcgac gtgtgcccga tcatccccgg cgtccgctac
     7141 gaggactggg cggtcggaga cccggccctc gcctccgtcg agggcgtcga ggccatccgc
     7201 gacgacatcg ccgcccgcgt ccgcaccctg ctcgactccc tcaccagccg ctaacgaaag
     7261 agacccgatc atgaccgacg ccaagccgtc cgtgctgttc gtctgcatcc acaacgccgg
     7321 ccgctcccag atggcagccg gctacctccg ccacctcgcc ggcgaccgca tcgaggtccg
     7381 ctccgccggc tccatgcccg ccgaccagat caaccccatc gccgtcgaag ccatgcgtga
     7441 agagggcatc gacatcaccg cagagcagcc caaggtgctc acgcccgagg ctgtccaggc
     7501 ctcggatgtg gtgatcacga tggggtgcgg cgacgcctgc ccctactacc cgggcaagcg
     7561 ctacgaggac tggaagctcg acgaccccgc cgggcaaggc atcgacgcgg ttcggccgat
     7621 ccgcgacgac atccgtgccc gtattgaggg gctgatcgcc tctctcg
//
LOCUS       DAOSUG010000075         7665 bp    DNA     linear   ENV 10-JUN-2024
DEFINITION  MAG TPA_asm: Micropruina sp. isolate AMR_MDS_2195
            NODE_4828_length_7665_cov_3.962681, whole genome shotgun sequence.
ACCESSION   DAOSUG010000075 DAOSUG010000000
VERSION     DAOSUG010000075.1
DBLINK      BioProject: PRJNA925477
            BioSample: SAMN32864262
            Sequence Read Archive: SRR4236673
KEYWORDS    WGS; ENV; Metagenome Assembled Genome; MAG; Third Party Data; TPA;
            TPA:assembly.
SOURCE      Micropruina sp. (wastewater metagenome)
  ORGANISM  Micropruina sp.
            Bacteria; Bacillati; Actinomycetota; Actinomycetes;
            Propionibacteriales; Nocardioidaceae; Micropruina.
REFERENCE   1  (bases 1 to 7665)
  AUTHORS   Abdulkadir,N., Saraiva,J.P., Zhang,J., Stolte,S., Gillor,O.,
            Harms,H. and Rocha,U.
  TITLE     Genome-centric analyses of 165 metagenomes show that mobile genetic
            elements are crucial for the transmission of antimicrobial
            resistance genes to pathogens in activated sludge and wastewater
  JOURNAL   Microbiol Spectr 12 (3), e0291823 (2024)
   PUBMED   38289113
REFERENCE   2  (bases 1 to 7665)
  AUTHORS   Abdulkadir,N.
  TITLE     Direct Submission
  JOURNAL   Submitted (24-MAR-2023) Environmental Microbiology, Helmholtz
            Center for Environmental Research -UFZ, Permoserstr 15, Leipzig
            04318, Germany
COMMENT     The annotation was added by the NCBI Prokaryotic Genome Annotation
            Pipeline (PGAP). Information about PGAP can be found here:
            https://www.ncbi.nlm.nih.gov/genome/annotation_prok/
            
            ##Genome-Assembly-Data-START##
            Assembly Method        :: SPAdes v. 3.11.1
            Genome Representation  :: Full
            Expected Final Version :: Yes
            Genome Coverage        :: Not Applicable
            Sequencing Technology  :: Illumina MiSeq
            ##Genome-Assembly-Data-END##
            
            ##Genome-Annotation-Data-START##
            Annotation Provider               :: NCBI
            Annotation Date                   :: 05/04/2023 04:57:10
            Annotation Pipeline               :: NCBI Prokaryotic Genome
                                                 Annotation Pipeline (PGAP)
            Annotation Method                 :: Best-placed reference protein
                                                 set; GeneMarkS-2+
            Annotation Software revision      :: 6.5
            Features Annotated                :: Gene; CDS; rRNA; tRNA; ncRNA
            Genes (total)                     :: 2,776
            CDSs (total)                      :: 2,750
            Genes (coding)                    :: 2,714
            CDSs (with protein)               :: 2,714
            Genes (RNA)                       :: 26
            tRNAs                             :: 24
            ncRNAs                            :: 2
            Pseudo Genes (total)              :: 36
            CDSs (without protein)            :: 36
            Pseudo Genes (ambiguous residues) :: 0 of 36
            Pseudo Genes (frameshifted)       :: 2 of 36
            Pseudo Genes (incomplete)         :: 32 of 36
            Pseudo Genes (internal stop)      :: 2 of 36
            ##Genome-Annotation-Data-END##
FEATURES             Location/Qualifiers
     source          1..7665
                     /organism="Micropruina sp."
                     /mol_type="genomic DNA"
                     /submitter_seqid="NODE_4828_length_7665_cov_3.962681"
                     /isolate="AMR_MDS_2195"
                     /isolation_source="Activated sludge of municipal
                     wastewater treatment plants"
                     /db_xref="taxon:2737536"
                     /environmental_sample
                     /geo_loc_name="Canada"
                     /lat_lon="49.25 N 123.1 W"
                     /collection_date="2015-06-16"
                     /metagenome_source="wastewater metagenome"
                     /note="metagenomic"
     gene            complement(307..807)
                     /locus_tag="PLF56_03820"
     CDS             complement(307..807)
                     /locus_tag="PLF56_03820"
                     /inference="COORDINATES: ab initio
                     prediction:GeneMarkS-2+"
                     /note="Derived by automated computational analysis using
                     gene prediction method: GeneMarkS-2+."
                     /codon_start=1
                     /transl_table=11
                     /product="hypothetical protein"
                     /protein_id="HOQ52724.1"
                     /translation="MSVTPSARHRLVRLIVAAVIALSGALGATVVTAAPAHALCSTPA
                     MSGNWHNINSGTRSLTRVNVTFVCDDVRLCDTSGHCTGGESYFTLRPFGKCSPTDCDW
                     GTKKAATMSDGWQRATYGYSWATKYVWVKTYVYSGVTYLRVYTWTDFTAADGRTDYST
                     DEWMLK"
     gene            complement(943..1950)
                     /locus_tag="PLF56_03825"
     CDS             complement(943..1950)
                     /locus_tag="PLF56_03825"
                     /EC_number="5.2.1.8"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_013862653.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_function: GO:0003755 - peptidyl-prolyl cis-trans
                     isomerase activity [Evidence IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="FKBP-type peptidyl-prolyl cis-trans isomerase"
                     /protein_id="HOQ52725.1"
                     /translation="MPFAVSRAVQAVGCAAALLILAACGSSATPSASPSEASPSASPS
                     ASASATPTSTIKPSTNLDGITVKNDKFGEKPTITVKAPWVIDKTQVKVMTEGKGAVVN
                     PSYIWAYYQGVNGRTGKVFDGNFGTGKTAMFNLEQVVPGFKTALEGKRVGSRILVAMP
                     GKDGYDSQGGQASADIKVGDTLIFVIDIVETVLSGPTGKVITPPAGLPTVTEKDGKPV
                     ITLPKTDPPTKLIAQPLTEGTGNKVTAKDVIAVHYVAVSWKTGKVIEDKTSDIDGGLL
                     SSTIPGWQKGLVGKRVGQRVLLVLPPAYNYPQGSNSPPVTAGDTLVYVVDILYAAPQS
                     G"
     gene            complement(2408..3823)
                     /locus_tag="PLF56_03830"
     CDS             complement(2408..3823)
                     /locus_tag="PLF56_03830"
                     /inference="COORDINATES: protein motif:HMM:NF015119.2"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_process: GO:0010498 - proteasomal protein catabolic
                     process [Evidence IEA];
                     GO_process: GO:0019941 - modification-dependent protein
                     catabolic process [Evidence IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="proteasome accessory factor PafA2 family
                     protein"
                     /protein_id="HOQ52726.1"
                     /translation="MGQARPRRVFGVETEYGLVGRRAGTGSVRGQRLSGGEAAARLFA
                     PIEREHASSNVFLRNGGRLYLDVGSHPEYATAECASMADLLVHERAGDELLARLAARA
                     EVDGAAEGEPVQLRLFKNNVDSHGNSYGCHENYLIDRALDLPRLSAALTPFLVTRQVL
                     CGTGRWRPDGFSISQRAEVLHDELSSMTTRSRPLINTRDEPLADPARFRRLHVIAGDS
                     NLSEPSAALKVGSLLAVLAAVEAALQGAGPSVPDLELADVPGALRAVAHDPRAVLRRA
                     SGGTIGALDVQTEYLALAGDAPVASRWGRVLSCLQRGAEDELDGEVEWVTKRALLRRY
                     AESRGLRWADRRLAQVDLAFHEISPVPDGAPRGLFRLLEARGAAVRLTSHDDVERALI
                     APPSDTRAVLRGRLIRTAQAEHRRYSVDWQQFTAHDLPDGSGGRVDRAVTLGDPLTVA
                     DQAVDELTRAMASGWTVPQPG"
     gene            complement(3830..4567)
                     /locus_tag="PLF56_03835"
     CDS             complement(3830..4567)
                     /locus_tag="PLF56_03835"
                     /EC_number="5.4.99.-"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_002524162.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_function: GO:0009982 - pseudouridine synthase activity
                     [Evidence IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="pseudouridine synthase"
                     /protein_id="HOQ52727.1"
                     /translation="MSDDEGIRLQKVLAAAGLGSRRHCEQLIEDGLVEVNGKVVAEQG
                     RRVDPLADVIRVDGARIPPPRRHAYLMFHKPRGVVSTMDDPQGRSTISDYLGKRTKDG
                     LFHVGRLDTPTEGLLLITNDGEFAQRMAHPSHEISKTYIAEVEGVIENTTLRKLSRGL
                     TLEDGPVKPDQVKLIQRGGTRSIVQVVLHEGRNRIVRRMFETLGHPVRRLSRTAVGPV
                     RLGQLKVGELRELSRDELGALMDLAGL"
     gene            complement(4560..5345)
                     /gene="scpB"
                     /locus_tag="PLF56_03840"
     CDS             complement(4560..5345)
                     /gene="scpB"
                     /locus_tag="PLF56_03840"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_013862650.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_function: GO:0005515 - protein binding [Evidence IEA];
                     GO_process: GO:0007059 - chromosome segregation [Evidence
                     IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="SMC-Scp complex subunit ScpB"
                     /protein_id="HOQ52728.1"
                     /translation="MTEPAEPLLFDDEADEGDADAIAAPLEALLLMATEPVSTVELAQ
                     AVRAPVRVVERTLAGLADFYDQTQRGFELRYVAGGWRYWTRPEYAELIGEWLIEGQHA
                     KLSQAALETLAVVAYLQPISRSRVSAVRGVNVDGVLRTLVTRDLVHEVDRDPESGAML
                     FGTTDEFLQRLGLAGLDDLPPIAPHLPDASELDAELRAALNPSDHDASVDEGVHGTRP
                     GGRDGIDSEVEHAEAAPDQLPDSAQAEGRSLHLSHRSEGSFDE"
     gene            complement(5329..6180)
                     /locus_tag="PLF56_03845"
     CDS             complement(5329..6180)
                     /locus_tag="PLF56_03845"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_013862649.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_process: GO:0007059 - chromosome segregation [Evidence
                     IEA];
                     GO_process: GO:0051301 - cell division [Evidence IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="ScpA family protein"
                     /protein_id="HOQ52729.1"
                     /translation="MTAATTEAVPGFTVRLTNFEGPFDLLLQLISRHRLDITEVALSV
                     VTDEFLAHVKAGQAQRGPGRWDLEQTTQFIVVAATLLDLKAARLLPQGEVEDPEDLAL
                     LEARDLLFARLLQYRAFKRVSAWIAETLADQGRRHARPGGLEQQFRALLPELEFTATA
                     DQLAVLAARALTPEPAEQVSLTHLHQANVSVREQADIVVARLRELGAASFRELVADAA
                     ERLVVVARFLALLELFRERAVTFEQLTPLGELRVRWVAGDADAIEISAEFDTETDPQE
                     RSDDRTR"
     gene            complement(6177..6470)
                     /locus_tag="PLF56_03850"
     CDS             complement(6177..6470)
                     /locus_tag="PLF56_03850"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_011755939.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="hypothetical protein"
                     /protein_id="HOQ52730.1"
                     /translation="MPRRVALPGARELFRVTDSGDVAGLRPSGRVKHDEKITVYVSAD
                     ELVALEQARLTLRALHGVAVDRGRIVRAAIALAVADLDANGDDCDLIRQLDVP"
     gene            complement(6455..7375)
                     /locus_tag="PLF56_03855"
     CDS             complement(6455..7375)
                     /locus_tag="PLF56_03855"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_015070773.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_function: GO:0005524 - ATP binding [Evidence IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="ParA family protein"
                     /protein_id="HOQ52731.1"
                     /translation="MTDTTVETPALFETAADVDRDLESGPELGPTGRPLPDLPEPQPP
                     ADGPTRALIIALCNQKGGVGKTTTTINLGAALAETGRRVLLVDFDPQGSLSVGLGVNP
                     HTLDTSIYNLLLTRDHDVHDIITASVVDGLDILPANIDLSAAEVQLVSEVAREQTLKR
                     VLAQVRDEYDIILIDCAPSLGLLTVNALTAADRVIMPLECEFFALRGLALLNDTIGKV
                     QDRLNPDLQILGILGTMYDARTLHSREVLTTVVEAFGDKVFHTVIRRTIKFPETTVAG
                     EPITTYASSSPGAEAYRMLAREVLQRCPGA"
     gene            complement(7448..>7665)
                     /locus_tag="PLF56_03860"
     CDS             complement(7448..>7665)
                     /locus_tag="PLF56_03860"
                     /inference="COORDINATES: protein motif:HMM:TIGR01167.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_component: GO:0009986 - cell surface [Evidence IEA]"
                     /codon_start=3
                     /transl_table=11
                     /product="LPXTG cell wall anchor domain-containing
                     protein"
                     /protein_id="HOQ52732.1"
                     /translation="TETPTAPTETPTPPTPTSTQPTGPSTGTTTPATNLPQTGSSASL
                     TVVWVGAALVIVGLTALIGARLARRRN"
BASE COUNT         1126 a         2660 c         2653 g         1226 t
ORIGIN      
        1 gccggcctgc ctcgccggcc gtggctgttc acgggccgct gaatggcatt cctggttatt
       61 ggacgctgcc gcagcgtgat catgggcagt ccggtgctgg tgtcgcggag tcagcatcgt
      121 cgaacgttgc ccacccacgt gaggcacgcg acaccggttg tcggtgccgc gagccttcag
      181 tgagaaagac ctgtcgtcac caggcagagt gtcacccgac tccgtcagga gtcccacgca
      241 gtgggcgccc cctcgcgcag gcccgtgggc gcccctcgcg cagtacgtgg gcgatggttg
      301 ccccgtctac ttcagcatcc actcgtcggt ggagtagtcg gtgcgtccgt cggcggcggt
      361 gaagtccgtc caggtgtaga cgcgcaagta ggtgacgccg ctgtacacgt aggtcttgac
      421 ccacacgtac ttggtggccc agctgtagcc gtaggtggcg cgctgccagc cgtccgacat
      481 ggtggccgcc ttcttggttc cccagtcgca gtcggtcggt gagcacttgc cgaacggacg
      541 cagggtgaag tagctctcgc cgccggtgca gtggccactg gtgtcgcata accgcacgtc
      601 atcgcagacg aaggtcacgt tcacccgggt cagcgagcgg gtgccggagt tgatgttgtg
      661 ccagttgccg gacatggccg gggtggaaca cagcgcgtgc gccggggcgg cggtgacgac
      721 ggtggcgccc agcgctccgg acagggcgat caccgcggcg acgatcagcc ggactaggcg
      781 gtgtcgggcg gaaggggtga cggacatggc agttcctccg gtttcgcgcg cccggggttg
      841 ggccgcgcgt accaggaaga tcgcgatcac cggccggcag atacccgctg ctgctacgca
      901 ctgctaccca tgccgtgtcg ctctggtacg agaggttccc gcctagcctg actgcggggc
      961 cgcgtagagg atgtcgacga cgtagaccag cgtgtcgccg gcggtgaccg gcgggctgtt
     1021 gctgccctgg gggtagttgt aggccggcgg cagcaccagc agcacgcgtt gtccgacccg
     1081 cttgccgacc agacccttct gccagcccgg aatcgtgctg gacaacaggc cgccgtcgat
     1141 gtcggaggtc ttgtcttcga tcaccttgcc ggtcttccac gacacggcca cgtagtgcac
     1201 ggcgatgacg tccttggcgg tgaccttgtt gccggtgccc tcggtgagcg gctgcgcgat
     1261 gagcttggtg ggcgggtcgg tcttgggcag cgtgatcacc ggcttgccgt ccttctcggt
     1321 gacggtgggt agcccggcgg ggggtgtgat caccttgccg gtcgggccgc tcagcaccgt
     1381 ttcgacgatg tcgatcacga agatcagggt gtcgccaacc ttgatgtcgg cactcgcctg
     1441 gccgccctgc gagtcgtagc cgtccttacc gggcattgcg accagaatgc ggctgccgac
     1501 ccgcttgccc tcgagtgcgg tcttgaagcc gggcaccacc tgctcgaggt tgaacatggc
     1561 cgtcttgccg gtgccgaagt tgccgtcgaa caccttgccg gtgcgtccgt tgacaccctg
     1621 gtagtacgcc cagatgtagg acgggttcac caccgcgccc ttgccctcgg tcatcacctt
     1681 gacctgcgtc ttgtcgatca cccagggcgc cttcacggtg atcgtcggct tctcaccgaa
     1741 cttgtcgttc ttcaccgtga tgccgtccag gttggtggag ggcttgatcg tcgaggtggg
     1801 cgtcgcgctc gccgaggccg acggggacgc cgacggagag gcttcggagg gcgacgccga
     1861 cggtgtggcg gacgaaccac aggcggcgag gatcagcagc gcggcggcgc agccgaccgc
     1921 ttggacggcg cgagaaacag caaaaggcac cccccgactg tagtcgacgc aggggaaccg
     1981 tccggccagg gccacccacg gctttgtcgt ccgtacccgt ggtgccgacc gcagtaaccc
     2041 ggatgacgag taccccgctg gacgtgacct ccttacggcg ctggtcgaca ccaccgtcat
     2101 cccggcgcac gccgggatct ccccgcacgc cggttccctg cgtgccccgt catcccgggg
     2161 caagccccgg atgacgagta ccccaccggc caaggccccc gcacaccgct ggccgacacc
     2221 accgtcatcc cggcgcacgc cgggatctcc gcgcgcgccg gttccctggg tgccccgaga
     2281 tcccggggca agcccgggat gacgagtact ttgcgcacta gatcggcgtc ttcccgcacc
     2341 gtgctcagaa ccgtccccga gtgttggggt cgcgccgcgc tgcttgcccg gcccgccgcc
     2401 cggctggtca gccgggttgt ggcaccgtcc agcccgacgc catcgccctg gtcagttcgt
     2461 ccaccgcctg gtcggccacc gtcagcgggt caccaagagt gactgcacgg tcgacgcgtc
     2521 cgccggagcc gtcgggtaaa tcgtgcgccg tgaactgctg ccagtcgacg ctgtagcgac
     2581 ggtgttctgc ttgcgcggtg cggatcaacc ggccccgcag caccgcccgg gtgtcggacg
     2641 ggggcgcgat cagcgcccgc tcgacgtcgt cgtgcgaggt caatcggacg gccgccccgc
     2701 gcgcctccag cagccggaac agtccgcgtg gggccccgtc cggcaccggg ctgatctcgt
     2761 ggaacgccag atcgacctgc gccagccggc gatcagccca cctcaggccg cgactctcgg
     2821 cgtagcgccg cagcagggcc cgcttggtca cccattcgac ctcgccgtcc aactcgtcct
     2881 cggcaccgcg ctgcaggcac gacagcaccc ggccccaccg gctcgctacc ggggcgtcac
     2941 cggccagcgc caggtactcg gtctgtacgt cgagtgcgcc gatggtgcca ccggaggcac
     3001 gcctcagcac cgcccgaggg tcgtgggcca ccgcccgcag cgcccccggc acgtcggcca
     3061 gttcgaggtc gggcacggac ggccccgcac cctgcagcgc cgcctcgacc gccgccagca
     3121 ctgccagcag cgacccaacc ttgagtgccg ccgacggctc cgacaggttc gaatcgcccg
     3181 cgatcacgtg cagtcggcgg aaccgcgcgg ggtcggccag cggctcgtcg cgggtgttga
     3241 tcaacggacg gctgcgcgtg gtcatcgacg acaactcgtc atgcaacacc tcggcgcgct
     3301 ggctgatcga gaaaccgtcc gggcgccagc gtccggtgcc gcacagcacc tgccgggtga
     3361 ccagaaacgg tgtcagcgcg gcggacagcc ggggtagatc gagcgcgcgg tcgatcagat
     3421 agttctcgtg acagccgtag gagttgccgt gactgtcgac gttgttcttg aacagccgca
     3481 gctgcaccgg ctcgccttca gcggcgccgt ccacctcggc ccgagccgcc agcctggcca
     3541 gcagctcgtc gccggcccgt tcgtgcacca gcaggtcggc catgctcgcg cactcggcgg
     3601 tggcgtactc ggggtgcgag ccgacgtcga gatagagccg tccgccgttg cgcagaaaca
     3661 cgttcgaact ggcgtgctcg cgctcgatgg gtgcgaacag ccgcgcggcc gcctcgccgc
     3721 cgctgagccg ttgcccacgg acggatcccg taccggcgcg ccgtccgacc aggccgtact
     3781 cggtctcgac gccgaacacc cgccgcggac gcgcctgccc cacgatcgcc tacagcccgg
     3841 ccaggtccat cagggccccc agctcgtcac ggctgagctc gcgcagctca ccgaccttca
     3901 actgcccgag ccggacgggc cctaccgcgg ttcgactcag ccgccggacc gggtgcccca
     3961 gcgtctcgaa catgcgccgg acgatgcggt tgcggccctc gtgcagcacc acctggacga
     4021 tgctgcgggt gccgccgcgt tgaatcagct tgacctggtc gggcttgacc ggaccgtctt
     4081 cgagggtgag gccgcggctc aacttgcgca gggtggtgtt ctcgatcacg ccctcgacct
     4141 cggcgatgta ggtcttgctg atctcgtgcg aggggtgcgc catgcgctgg gcgaactcgc
     4201 cgtcgttggt gatcagcagc aggccctcgg tgggggtgtc gagccgtccg acgtggaata
     4261 gtccgtcctt ggtgcgcttg cccaggtagt ccgagatcgt gctgcggccc tgcggatcgt
     4321 ccatggtcga gaccaccccg cgcggcttgt ggaacatcag gtacgcgtgc cggcgcgggg
     4381 gcgggatgcg cgccccgtcc acccggatca cgtcggccaa cgggtcgacc cggcgcccct
     4441 gctcggcgac caccttgccg ttcacctcga ccaggccgtc ctcgatcagt tgctcgcagt
     4501 gccgccgcga accgagcccg gccgcagcca gcaccttctg caggcgaatg ccctcgtcgt
     4561 cactcatcga aactcccctc ggaacggtgg gacaggtgca gagagcggcc ctcggcctgc
     4621 gcactgtcgg gcaactgatc cggagccgcc tcagcgtgct ccacttcgct atcgatacca
     4681 tcgcgcccgc ccggacgcgt gccgtgcacg ccctcgtcaa cggacgcatc atggtcggac
     4741 gggttcagcg cggcccgcaa ctccgcgtcc agttcggagg cgtcgggcag gtgcggcgcg
     4801 atcggtggca ggtcgtcgag gccggccagc ccgagccgtt gcagaaactc gtcggtggtg
     4861 ccgaacagca tcgcccccga ctccgggtct cgatccacct cgtgcaccag gtcgcgggtg
     4921 accagggtgc gcagcacacc gtccacgttg acgccgcgga cggccgagac ccggctacgc
     4981 gagatgggct gcaggtaggc gaccacggcc agcgtctcga gcgccgcctg cgacaacttg
     5041 gcgtgctggc cctcgatcag ccactcgccg atcagctcgg cgtactcagg acgcgtccag
     5101 tagcgccagc cgccggccac gtagcgcaac tcgaacccgc gctgggtctg gtcgtagaag
     5161 tcggccagcc cggccagggt gcgctcgacg acccgcaccg gcgcccggac ggcctgcgcc
     5221 agctcgacgg tcgacaccgg ctcggtggcc atcagcagca gcgcctccag gggtgcggcg
     5281 atggcgtcgg cgtcgccttc gtcggcctcg tcgtcgaaga gcagtggttc agcgggttcg
     5341 gtcatcgctg cgctcctgag gatcggtttc ggtgtcgaac tcggcggaga tctcgatcgc
     5401 gtccgcgtcg ccggcgaccc accgtactcg caactccccc agcggggtga gttgctcgaa
     5461 cgtgacggcg cgttcgcgga acagttccag cagggccaga aaccgcgcca ccacgaccag
     5521 ccgttcggcg gcatcggcca ccagctcacg gaagctggcc gcacccagtt cacgcagccg
     5581 tgccaccacg atgtcggcct gctcacgcac gctgacgttg gcctggtgca ggtgcgtcaa
     5641 cgacacctgt tcggccggct cgggggtgag cgcccgggcc gcgagcaccg ccaactggtc
     5701 ggcggtggcg gtgaattcca gttcgggcag cagcgcccgg aactgttgtt ccaacccgcc
     5761 cggacgcgcg tgccgccgtc cctggtcggc cagcgtctcg gcgatccagg cgctgacccg
     5821 tttgaacgcc cggtactgca gcagccgcgc gaacagcagg tcgcgggcct cgagcagggc
     5881 caggtcctcg ggatcctcca cctcaccttg cggcagcagc cgcgccgcct tgaggtcgag
     5941 cagggtggcc gccaccacga tgaactgagt ggtctgttcc agatcccagc gacccggccc
     6001 gcgctgcgcc tgcccggcct tgacgtgggc caaaaactcg tccgtgacaa cggacaacgc
     6061 cacctcggtt atgtcgagcc ggtggcgcga gatcagctgc agcagcagat cgaaggggcc
     6121 ctcgaagttg gtcagccgaa cggtgaagcc gggcaccgcc tcggtcgtgg cggcggtcac
     6181 ggaacgtcga gttggcggat caggtcacag tcgtcgccgt tggcgtccag gtcggccacg
     6241 gccagggcga tcgccgcacg cacgatgcgt ccgcgatcta ccgcgacccc gtgcagggcg
     6301 cgcagggtga gccgagcctg ctcaagggcg accagctcat cggcactgac gtagacggtg
     6361 atcttctcgt cgtgcttgac ccgcccggac ggacgcagcc cggcgacgtc gccggagtcg
     6421 gtgacgcgga acagttcgcg cgcacccgga agcgctacgc gccggggcat cgctgcagca
     6481 cctcccgggc cagcatgcgg tacgcctcgg cgcccggcga actggacgcg taggtggtga
     6541 tcggctcgcc ggccacggtg gtttcgggaa acttgatcgt gcgcctgatg accgtgtgaa
     6601 acaccttgtc gccgaacgct tccacgaccg tggtcagcac ctcgcgcgaa tgcagggtgc
     6661 gggcgtcgta catagtgccg agaatgccca gaatctgcag gtcggggttc agccggtcct
     6721 gcaccttgcc gatggtgtcg ttgagcaggg ccaggccgcg cagcgcgaag aactcgcatt
     6781 ccagcggcat gatcacccga tcggcggcgg tcagcgcgtt gacggtgagc aggcccaggg
     6841 acggcgcaca gtcgatcaga atgatgtcgt actcgtcgcg cacctgggcg agcactcgct
     6901 tgagggtctg ctcccgcgcc acctcgctga ccagttggac ctcggcggcc gacaggtcga
     6961 tgttggccgg cagaatgtcg agaccgtcga ccacggaggc ggtgatgatg tcgtgcacgt
     7021 cgtggtcgcg ggtgagcagc aggttgtaga tgctggtgtc gagggtgtgc ggattcaccc
     7081 ccaggccgac cgacagcgac ccctgcggat cgaagtcgac cagcagcacc cggcgtccgg
     7141 tttcggccag tgcggcaccc aggttgatcg tggtggtggt cttgcccacc ccgcccttct
     7201 ggttgcacag cgcgatgatc agtgcccggg tggggccgtc ggccggcggc tgcggttcgg
     7261 gcaagtcggg cagcggacgt ccggtgggcc cgagttcggg gccggattcg aggtcgcggt
     7321 cgacgtccgc ggcggtctcg aacagggctg gggtctcgac ggtggtgtcg gtcatgaagc
     7381 ctccaagtgt cgataaagag gctaataccc tttgaccggg agcccggacg ccacgccgcg
     7441 cgcgtgctca gttgcggcgc cgcgccagcc tggccccgat cagggcggtc agcccaacga
     7501 tcaccagggc cgcgccgacc cacacaaccg tgaggctggc actgctgccg gtctgcggca
     7561 ggttggtcgc cggtgtggtg gtgccggtgg acggaccggt cggttgcgtg gaggtcgggg
     7621 tcggcggggt cggagtctcg gtgggcgcgg tcggggtctc ggtcg
//
LOCUS       DAOSUG010000076         7649 bp    DNA     linear   ENV 10-JUN-2024
DEFINITION  MAG TPA_asm: Micropruina sp. isolate AMR_MDS_2195
            NODE_4833_length_7649_cov_3.988675, whole genome shotgun sequence.
ACCESSION   DAOSUG010000076 DAOSUG010000000
VERSION     DAOSUG010000076.1
DBLINK      BioProject: PRJNA925477
            BioSample: SAMN32864262
            Sequence Read Archive: SRR4236673
KEYWORDS    WGS; ENV; Metagenome Assembled Genome; MAG; Third Party Data; TPA;
            TPA:assembly.
SOURCE      Micropruina sp. (wastewater metagenome)
  ORGANISM  Micropruina sp.
            Bacteria; Bacillati; Actinomycetota; Actinomycetes;
            Propionibacteriales; Nocardioidaceae; Micropruina.
REFERENCE   1  (bases 1 to 7649)
  AUTHORS   Abdulkadir,N., Saraiva,J.P., Zhang,J., Stolte,S., Gillor,O.,
            Harms,H. and Rocha,U.
  TITLE     Genome-centric analyses of 165 metagenomes show that mobile genetic
            elements are crucial for the transmission of antimicrobial
            resistance genes to pathogens in activated sludge and wastewater
  JOURNAL   Microbiol Spectr 12 (3), e0291823 (2024)
   PUBMED   38289113
REFERENCE   2  (bases 1 to 7649)
  AUTHORS   Abdulkadir,N.
  TITLE     Direct Submission
  JOURNAL   Submitted (24-MAR-2023) Environmental Microbiology, Helmholtz
            Center for Environmental Research -UFZ, Permoserstr 15, Leipzig
            04318, Germany
COMMENT     The annotation was added by the NCBI Prokaryotic Genome Annotation
            Pipeline (PGAP). Information about PGAP can be found here:
            https://www.ncbi.nlm.nih.gov/genome/annotation_prok/
            
            ##Genome-Assembly-Data-START##
            Assembly Method        :: SPAdes v. 3.11.1
            Genome Representation  :: Full
            Expected Final Version :: Yes
            Genome Coverage        :: Not Applicable
            Sequencing Technology  :: Illumina MiSeq
            ##Genome-Assembly-Data-END##
            
            ##Genome-Annotation-Data-START##
            Annotation Provider               :: NCBI
            Annotation Date                   :: 05/04/2023 04:57:10
            Annotation Pipeline               :: NCBI Prokaryotic Genome
                                                 Annotation Pipeline (PGAP)
            Annotation Method                 :: Best-placed reference protein
                                                 set; GeneMarkS-2+
            Annotation Software revision      :: 6.5
            Features Annotated                :: Gene; CDS; rRNA; tRNA; ncRNA
            Genes (total)                     :: 2,776
            CDSs (total)                      :: 2,750
            Genes (coding)                    :: 2,714
            CDSs (with protein)               :: 2,714
            Genes (RNA)                       :: 26
            tRNAs                             :: 24
            ncRNAs                            :: 2
            Pseudo Genes (total)              :: 36
            CDSs (without protein)            :: 36
            Pseudo Genes (ambiguous residues) :: 0 of 36
            Pseudo Genes (frameshifted)       :: 2 of 36
            Pseudo Genes (incomplete)         :: 32 of 36
            Pseudo Genes (internal stop)      :: 2 of 36
            ##Genome-Annotation-Data-END##
FEATURES             Location/Qualifiers
     source          1..7649
                     /organism="Micropruina sp."
                     /mol_type="genomic DNA"
                     /submitter_seqid="NODE_4833_length_7649_cov_3.988675"
                     /isolate="AMR_MDS_2195"
                     /isolation_source="Activated sludge of municipal
                     wastewater treatment plants"
                     /db_xref="taxon:2737536"
                     /environmental_sample
                     /geo_loc_name="Canada"
                     /lat_lon="49.25 N 123.1 W"
                     /collection_date="2015-06-16"
                     /metagenome_source="wastewater metagenome"
                     /note="metagenomic"
     gene            <1..1046
                     /locus_tag="PLF56_03865"
     CDS             <1..1046
                     /locus_tag="PLF56_03865"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_013861089.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_function: GO:0000976 - transcription cis-regulatory
                     region binding [Evidence IEA];
                     GO_function: GO:0003700 - DNA-binding transcription factor
                     activity [Evidence IEA];
                     GO_function: GO:0043565 - sequence-specific DNA binding
                     [Evidence IEA];
                     GO_process: GO:0006351 - DNA-templated transcription
                     [Evidence IEA];
                     GO_process: GO:0006355 - regulation of DNA-templated
                     transcription [Evidence IEA];
                     GO_process: GO:0045892 - negative regulation of
                     DNA-templated transcription [Evidence IEA]"
                     /codon_start=3
                     /transl_table=11
                     /product="LacI family DNA-binding transcriptional
                     regulator"
                     /protein_id="HOQ52733.1"
                     /translation="DRAGVSVATASRSLGDGAKVSPRTRSRVLDAARELDYDTTRRRS
                     AAAGQGRRAIAIIVPFITRWFFAKAAVAALEVLRAAGYDVEIHHLGNASVRDDFFERT
                     PLAARVDGILSLSMPLTAEHTLALRALGLPLVSIGSHIDGFPSVGINDVDGARQAVHH
                     LLNLGHRRIGLICGKPDDTRFDFTASMSRRLGYEEALASAGIEFDDRLVVEGPHGVNG
                     GALAMTELMARPRLPSAVLAEFDELALGALWAIRRAGLRVPQDISIVGIDDIEMAEFV
                     DLTTVAQDVEAQGRTAARMLLRMLGDEPESVDAQPVRHLVHLILRGSSAPPAVQPDDR
                     VPPVEHPGTTVRL"
     gene            1190..1690
                     /locus_tag="PLF56_03870"
                     /pseudo
     CDS             1190..1690
                     /locus_tag="PLF56_03870"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_002543917.1"
                     /note="incomplete; partial in the middle of a contig;
                     missing C-terminus; Derived by automated computational
                     analysis using gene prediction method: Protein Homology."
                     /pseudo
                     /codon_start=1
                     /transl_table=11
                     /product="hypothetical protein"
     gene            complement(2176..3162)
                     /locus_tag="PLF56_03875"
     CDS             complement(2176..3162)
                     /locus_tag="PLF56_03875"
                     /inference="COORDINATES: protein motif:HMM:NF018028.2"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="RDD family protein"
                     /protein_id="HOQ52734.1"
                     /translation="MSNQTWNNDPKPEGSGDDSGAAGSIPPSYTPPGADPNQGQTFGY
                     QPPAWNGQQAAPSGTSGAEAPGQGGYQPPSYGAPGQPSYAPSGSEAPGQGGYAPPNYG
                     APSTPDQTFGQTPSYGSPSQPPYSQPQGGQGQAPSAQTPYGAVPQVGYDPNATGGAFG
                     QPQAPYGAPGGYGAPAPYGAPGSYQPNPYAPAGPQLATWGRRAIGGLVDFVVPAFAIS
                     ILIGVLMPNGGYGANLISTVLTLGWWAYNTGYRVATTGYSFGQKLGKVRVVMEDTGQT
                     PSQNVAITRALSHFLDSLCLIGFLFPLWDSKKQTFADKIAKTVVIDEQNLQR"
     gene            3263..3667
                     /locus_tag="PLF56_03880"
     CDS             3263..3667
                     /locus_tag="PLF56_03880"
                     /inference="COORDINATES: protein motif:HMM:NF022281.2"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="DUF2752 domain-containing protein"
                     /protein_id="HOQ52735.1"
                     /translation="MRSETATSNGFRAGTALRGLAAFGATGVGLSVVAATTGVGVPCP
                     WRRLTGTLCPFCGGTQVGVALLHGDPAAAWAANQFVLAGLVLLTLLGALWTVEALGGP
                     SVRPPRVLRFGSGRGWLLIGVLALAFAVWRNF"
     gene            complement(3775..5091)
                     /locus_tag="PLF56_03885"
     CDS             complement(3775..5091)
                     /locus_tag="PLF56_03885"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_012923732.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_function: GO:0004834 - tryptophan synthase activity
                     [Evidence IEA];
                     GO_function: GO:0030170 - pyridoxal phosphate binding
                     [Evidence IEA];
                     GO_process: GO:0000162 - tryptophan biosynthetic process
                     [Evidence IEA];
                     GO_process: GO:0006568 - tryptophan metabolic process
                     [Evidence IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="TrpB-like pyridoxal phosphate-dependent enzyme"
                     /protein_id="HOQ52736.1"
                     /translation="MSAPTKTKVVLDESQIPTHWYNIVADLPEAPPPPLHPGTKEPLT
                     PDDLAPLFPMGLIEQEASTERWIEIPQAVRDIYRLWRPSPMIRAWRLEQALGTTARIY
                     YKYEGASPVGSHKTNSAVPQAYFNKLAGRTKLTTETGAGQWGSALAFACDVFDLECEI
                     WQVRSSYEGKPYRHVLMETFGATVHSSPSELTATGRALRERFPDTTGSLGMAISEAIE
                     VAAGDPNASYSLGSVLNHVVLHQSVIGQEALLQLAEFGEQQADVVYGCAGGGSNLAGI
                     AFPFLRENLDGRTKTRLVACEPKAAPSLTEGEYRYDFGDTAGMTPLLKMYTLGPDFVP
                     DPVHAGGLRYHGMAPLVSFVKHLGLLDAISVKQREAFAAGVLFARTEGIVPASESNHA
                     IAGALREARQAADDGTSPVIVIGVSGSGQLDLPAYSEYLAGQMADS"
     gene            5163..5870
                     /locus_tag="PLF56_03890"
     CDS             5163..5870
                     /locus_tag="PLF56_03890"
                     /inference="COORDINATES: ab initio
                     prediction:GeneMarkS-2+"
                     /note="Derived by automated computational analysis using
                     gene prediction method: GeneMarkS-2+."
                     /codon_start=1
                     /transl_table=11
                     /product="hypothetical protein"
                     /protein_id="HOQ52737.1"
                     /translation="MIDLTIVELRDAAQNPGLVESAYTHVLRPSFTSDELPGPGGVRP
                     GPARVLTVAVDGAGVPVGAAVTELEPEGIGLLAYLAVAPAGRGLGVGGRLVAELHALW
                     RAEDQSLVLAEVHDPRGHAESDDEHPEARLRFYGRAGAQLLDVPWVQPGLGGGARVPD
                     MLLLAVHRTDPERPDVASAPLVAWTREYFTESEGREPDDAQAVALLARIGADERIRVL
                     PLGEWRRVERLAALPTA"
     gene            complement(5959..>7649)
                     /locus_tag="PLF56_03895"
     CDS             complement(5959..>7649)
                     /locus_tag="PLF56_03895"
                     /inference="COORDINATES: ab initio
                     prediction:GeneMarkS-2+"
                     /note="Derived by automated computational analysis using
                     gene prediction method: GeneMarkS-2+."
                     /codon_start=3
                     /transl_table=11
                     /product="hypothetical protein"
                     /protein_id="HOQ52738.1"
                     /translation="ASPVVAPGLRPRRAAQRCRDFENSPAGSWSVFATEVRAQRIPLE
                     AAADALEARAETLALRAADVTYLYPFGLPVAATGADVLPELMKALERDGHDDRARSPV
                     GALAGARVVIDDAPRSDGWLASSAVQEQDYRTFRAVLPDHELMLTTADGVTYAGLDLD
                     VRLSGMGNHYVRLRLGTDTRQEGRGAAPAPQPWTPHELEQAIRRGGQYAGAERVWLRA
                     RASADADGAQFPSLLALATRIVADLARAAHAHEGSGPDELGRDLAVAEGFLARYCQVL
                     VTVSRAEAVAPGGGRRALVTETDLEGVLGVQTALTAQRSLAQSALEWVAYPSADPDLR
                     RRRTLAGRIQREIVWCAGDLTALFVPTAPHWRSVATQAHVQYASSLIGVYWRRQQWLR
                     SVVDEVGAVLDTDDSDLDPARLAACAAKLSEAIRHVQLLVDRANEYTLSKEQQAREVL
                     QHLMGLNGVADLRRSLDATVEAAQSTQRSLTERLTEARASAEEAGRHRRQRPLEILLA
                     VLAVVGLIDVWMWVNEGWSLTGDRGWWIAESAVVVAGALVLWWLGRRAWQRGIS"
BASE COUNT         1103 a         2666 c         2703 g         1177 t
ORIGIN      
        1 cggaccgcgc gggggtctcg gtggccaccg cgtcccgctc cctcggcgac ggggccaagg
       61 tctcgccgcg aacccgaagc cgggtgttgg acgccgcccg cgaactcgac tacgacacga
      121 ccaggcgcag gtctgcggcc gccggccagg gccgccgggc catcgccatc atcgtcccct
      181 tcatcaccag atggtttttc gcgaaagcgg cggtcgccgc gctggaggtg ctgcgggccg
      241 caggctacga cgtggagatc catcacctcg gcaatgcgag cgtgcgcgac gactttttcg
      301 aacggacgcc gctggcggcc cgcgtcgacg gcatcctttc gctgtcgatg ccgctcaccg
      361 ccgagcacac gctcgcccta cgcgcgctcg gcctgcccct ggtcagcatc ggctcccaca
      421 tcgacggctt tccgtcggtc gggatcaacg atgtcgacgg cgcgcggcag gccgtgcatc
      481 acctgctgaa tctgggtcac cggcggatcg gcctgatctg cggcaaaccg gacgacacca
      541 ggttcgactt caccgcgtcg atgagtcgcc gcctgggcta cgaggaggcg ctggcgtcgg
      601 cggggatcga gttcgacgac cggctggtgg tcgagggccc gcacggcgtc aacggaggcg
      661 ccctggcgat gaccgagttg atggcccggc cgaggttgcc gagtgcggtg ctggccgagt
      721 tcgacgagct cgcgctcggt gcgctgtggg ccatccggcg ggcaggactg cgggtgcccc
      781 aggacatctc gatcgtcggc atagacgaca tcgagatggc cgagttcgtc gacctgacca
      841 cggtggccca ggacgtcgag gcgcagggcc ggacggcggc ccggatgctg ctgcggatgc
      901 tgggcgacga gccggaaagc gtcgatgccc aaccggtccg gcacctcgtg cacctcatcc
      961 tgcgcggcag cagcgctccg cccgcggtcc agccggacga tcgtgtgcct ccggtcgagc
     1021 accctggcac aacggtgcga ctctgaccgt gtgcggccgc cgaccggcga cgccgagtcc
     1081 gattagcggt gggaagaatg acattcaccg cgcacggcat cacaatgggt cttggtgtcc
     1141 ggccggttcg gatgccgcgc cacgatgacc ggcagacgaa aggtttccga tgacccagcc
     1201 gaatccccgg ctcagttccc tgttcgaact cgagtttcca caatcggtcg gcgtatacga
     1261 cagctacccg caggcgcagg cagccgtcga cttcctggcg gatgcgaagt tccccgtaga
     1321 gaacgtggcg atcgtcggca ccgatctcaa gtccgtcgaa cgggtgaccg gacggagaaa
     1381 ctggggcacg gtgatggtca gcggagtcca gaccggtatc tcgaccggcc tgatggtggc
     1441 cttcatcctg tggttcctgc agccgcagac caatccgttg atactgcttc ccggcgcact
     1501 gctgatcggc atcctgatcg gcatcgtgtt cgccgcgatc gcctatgcgt cgtcgcgtgg
     1561 caagcgtgac ttcaactcga tcagccagac cttcgccacc cactacgagg tgctctgcga
     1621 gcacaaggtg gccggtcagg cccgtgagtt gctggccggc atgcccggag cccgcgccgc
     1681 tgctttcgac ccgcgcaatc agccgcactc gggcggctgg ccgcagccgc aggcccagta
     1741 ttcgccgccg cagttcccgc aacagcagta cccacaggcg ccccagccgg ccaagtaccc
     1801 gggccagccg cagtcgtacg gccaggcgcc ggcgccttac ccgcagaccc cgccgcaggc
     1861 tcacggccaa gcgccggcgc aaccttatgc acagccgtcg acgcccgcag gccaggatcc
     1921 ggcgccgccg caggccggcc agcaaccgta cggcacctac ggcagcgctg agggcttggg
     1981 gcaggcgccg tccggtcagg gcgcgtcggg cccggcaccg tccgaccctc cggaggcggc
     2041 atcggactcc gaccgtaccc cgcgcgccga tggatgagtc gaccttcagg cccgctgcgc
     2101 agcgggcgcg cgatcgcatc acaagcaaca agggccgccc ctcggggcgg cccttgcggt
     2161 gcttcgtggg cggggtcagc gctggaggtt ctgctcgtcg atgaccacgg tcttggcgat
     2221 cttgtcggcg aaggtctgct tcttggaatc ccacaggggg aacagaaagc cgatcaggca
     2281 cagcgagtcg agaaaatgcg acagcgcacg cgtgatggcc acgttctgcg acggcgtctg
     2341 cccggtgtct tccatcacca cccgaacctt gccgagcttc tgcccgaagg agtaccccgt
     2401 cgtggcgacc cggtagccgg tgttgtaggc ccaccaaccc aacgtcaaca cggttgagat
     2461 caggttggcg ccatacccgc cgttgggcat cagaacgccg atcaggatgc tgatcgcgaa
     2521 ggcgggcacg acgaagtcga ccagtccgcc gatcgcccgg cggccccacg tggccagctg
     2581 gggacccgcg ggcgcgtacg ggttgggctg gtagctgccc ggcgcgccgt acggtgcggg
     2641 cgccccataa ccgccgggtg caccgtaggg tgcttgcggc tgcccgaacg caccgccggt
     2701 ggcgttgggg tcgtagccga cctgtggtac ggcaccgtac ggcgtctgcg cactcggcgc
     2761 ctgcccctga ccgccctgcg gctggctgta gggcggctgg ctcggcgaac cgtaggaagg
     2821 ggtctggccg aaagtctggt cgggggtgct cggggcgccg tagttgggtg gcgcgtagcc
     2881 gccctggccg ggtgcttcac tgccgcttgg cgcatacgac ggctggcccg gcgcgccgta
     2941 actgggcggc tggtaaccgc cctggccggg tgcctcggca ccgctggtgc cgctgggggc
     3001 cgcctgttgg ccgttccagg ccggcggctg gtaaccgaac gtctggccct gattgggatc
     3061 ggcgccgggg ggcgtatagc tgggcgggat ggagccggcg gctccgctgt cgtcgcccga
     3121 gccttcgggc ttggggtcgt tgttccacgt ctgattgctc acgtgtctct cctgggtttc
     3181 gagatcaggt gttcgcattg tacggctgag cagggcgttg cctcgggtgc acggcgcaca
     3241 acttcggcga ataatggacg ccgtgcgctc tgaaactgcg acgtcgaacg gctttcgtgc
     3301 cgggacggcc ctgcgcggcc tggccgcctt cggcgcgacc ggcgtggggt tgagtgtggt
     3361 ggcggccacc acgggcgtgg gggtgccgtg cccgtggcgc aggctgaccg gcaccctatg
     3421 tccgttctgc ggcggcaccc aggtcggcgt ggcgctgctg cacggcgacc cggcagcggc
     3481 ctgggcggcc aaccagttcg tgctcgccgg cctggtgctg ctcacgctgc tcggagccct
     3541 gtggacggtc gaggcgctcg gtggaccgtc cgttcggccg ccccgagtgt tgcggttcgg
     3601 ctccggccgc gggtggctgc tgatcggagt gctcgccctg gcgttcgccg tgtggcgcaa
     3661 cttctgaggg cgatccgcgg gtccagcggg ccgacactcc tcgtcagggg gtctcgacca
     3721 gctcgaccag caataggacg gctggggcgg ttcagacgct caggcgcagg ttcgtcagct
     3781 gtccgccatc tgcccggcca aatactccga gtaggcgggc aggtcgagtt gacccgagcc
     3841 cgagacgccg atcacgatca ccggcgaggt gccgtcgtcc gccgcctgcc gcgcctcacg
     3901 cagtgcaccg gcgatcgcgt gattcgactc cgaggcaggc acgatgccct cggtccgggc
     3961 gaacaggacg ccggccgcga acgcctcgcg ctgcttgacg ctgatcgcgt ccagcaggcc
     4021 caggtgcttg acgaagctca ccagcggcgc catgccgtgg tagcgcaggc cgccggcgtg
     4081 caccgggtcg ggcacgaagt cgggccccaa ggtgtacatc ttcagcagcg gcgtcatgcc
     4141 cgcggtgtcg ccgaagtcgt agcggtactc gccctcggtc agcgacggcg ccgccttcgg
     4201 ctcgcaggcc accagccggg tcttcgtccg gccgtccagg ttctcgcgca ggaacgggaa
     4261 ggcgatgccg gccaggttcg agccgccccc ggcgcagccg tacaccacgt ccgcctgctg
     4321 ctcgccgaac tcggccagtt gcagcaatgc ctcctggccg atcacgctct ggtgcagcac
     4381 aacgtggttg agcaccgagc ccagcgagta ggaggcgttc gggtcgccgg cggccacctc
     4441 gatggcctcg gagatcgcca tgcccagcga ccctgtggtg tccggaaaac gctcgcgcag
     4501 agcacgtccg gtcgcggtca gctccgacgg cgacgaatgg acggtcgcgc cgaaggtctc
     4561 catcagcacg tgccggtacg gcttgccctc ataggacgac cggacctgcc agatctcgca
     4621 ttcgaggtcg aacacgtcgc aggcgaacgc cagcgccgaa ccccactggc cggccccggt
     4681 ctcggtggtg agcttggtgc gtccggccag cttgttgaag tacgcctgcg gcacggccga
     4741 gttggtcttg tgcgaaccga ccggcgaggc gccctcgtac ttgtagtaga tgcgggcggt
     4801 ggtgcccagt gcctgctcga gccgccaggc ccggatcatc ggcgagggcc gccacagccg
     4861 atagatgtcg cggacggcct gcggaatctc gatccagcgc tcggtactgg cctcctgctc
     4921 gatcaggccc atcgggaaca acggcgccag gtcgtccggc gtcaacggct ccttggtgcc
     4981 cgggtgcaat ggcggcgggg gagcctctgg cagatcggcc acgatgttgt accagtgtgt
     5041 ggggatctgg gattcgtcca gaaccacctt cgtcttcgta ggtgcgctca cgcgagcgag
     5101 gctagcggca tcgccgcccg aggcggcacg tggcccacgg tgcgggccac tagcctggcc
     5161 cgatgatcga cctcaccatc gtcgagctcc gcgacgccgc gcagaatccc gggctcgtcg
     5221 agagcgccta tacccacgtg ctgcggccgt cgttcaccag cgacgagctg cccggacccg
     5281 ggggtgttcg cccgggcccg gctcgggtgt tgaccgttgc cgtggacggc gccggcgtcc
     5341 cggtcggcgc cgcggtcacc gaactcgaac ctgagggaat cggtctgttg gcctacctcg
     5401 ccgtcgctcc ggcggggcgc ggcctcggcg tcggtggacg gctggtcgcc gaactgcacg
     5461 cgctctggcg cgccgaggac cagtctctgg tgctggccga ggtgcacgac cccagggggc
     5521 atgccgaatc cgacgacgag catcccgagg cccgactgcg gttctacggg cgcgccggtg
     5581 cccagttgtt ggacgtgccg tgggtgcagc caggcctggg cggcggcgcc cgagtgccgg
     5641 acatgctgct gctggcggtg caccggacgg accccgagcg gccggacgtg gcctccgcgc
     5701 cactggtggc ctggacccgg gagtacttca ccgaatccga gggccgcgaa cccgacgatg
     5761 ctcaggcggt ggcgctgctc gcgcggatcg gggccgacga acgcatccga gtgctgccgc
     5821 tgggcgagtg gcgtcgggtc gaacggttgg ctgcgttgcc aaccgcctga tcggcggccc
     5881 gaacgcgact gccccgacgc cacaacgtgc ggccggagtg gtcgtggacg cccgccaggc
     5941 tgcctcgcaa gcgtgcggct acgagatgcc gcgctgccac gcccgacgtc cgagccacca
     6001 cagcaccagc gcgccggcca ccaccacggc tgattcggcg atccaccagc cgcggtcgcc
     6061 ggtcaaactc cagccctcat tgacccacat ccacacgtcg atcaggccga ccaccgcgag
     6121 gaccgcgagc agaatctcca acggccgctg gcgccggtgt cgacccgcct cctcggcgga
     6181 ggctcgcgcc tcggtgagcc gttcggtgag cgatcgctgg gtgctctggg ccgcctcgac
     6241 cgtggcgtcc agcgaacggc gcagatccgc gacgccgttg agccccatca ggtgctgcaa
     6301 cacctcgcgc gcctgttgct ctttcgacag cgtgtactcg ttggcacggt cgaccagaag
     6361 ctgaacgtgc cggatcgcct cactcagctt ggccgcacag gctgccagcc gggcgggatc
     6421 gaggtctgaa tcgtcggtgt cgagcaccgc tccgacttcg tcgaccacac tgcgcaacca
     6481 ctgctgccgg cgccagtaga ccccgatcag gctggaggcg tactgcacgt gcgcctgggt
     6541 cgccaccgag cgccagtgcg gtgcggttgg gacgaacagt gcggtcaggt caccggcaca
     6601 ccagacgatc tcgcgctgga tccgtccggc cagggtacgg cgtcgccgca ggtcgggatc
     6661 agcactcggg taggccaccc attccagggc gctctgcgcc agggaacgct gcgcggtgag
     6721 tgcggtctgc acaccgagca cgccctcgag atcggtctcg gtcaccaggg cgcggcgccc
     6781 tccgccgggc gcgacggctt cggcccgcga cactgtcacc agcacctggc agtacctggc
     6841 gaggaatcct tccgccaccg cgaggtcgcg gccgagttcg tcaggaccac tgccctcatg
     6901 cgcatgcgcg gcccgggcca ggtcggccac gatgcgggtg gccagggcca gcagggaggg
     6961 gaactgcgca ccgtcggcat cggcactcgc gcgggcgcgc aaccagactc gttctgcccc
     7021 ggcgtactgg ccgccgcgcc gaatcgcctg ttccagttcg tgcggggtcc acggctgcgg
     7081 ggcaggagcc gcgccgcgac cctcctgtcg ggtgtcggtg ccgagtcgca gccggacgta
     7141 atgattgccc atgccgctca gtcgcacgtc gagatcgagc ccggcgtagg tgacgccgtc
     7201 ggcagtggtc agcatcagtt cgtgatcggg aaggacggcg cggaacgtgc ggtagtcctg
     7261 ttcctgcacg gcggacgagg cgagccagcc gtccgaccgc ggcgcgtcgt cgatcaccac
     7321 cctggccccg gccagtgcgc cgaccggtga acgggcccgg tcgtcgtggc cgtcgcgttc
     7381 cagtgccttc atcagctcgg gcagcacgtc ggcgccggtc gccgccaccg gcagcccgaa
     7441 cgggtagagg taggtgacat cggcggccct gagggccaac gtctcggcac gcgcctccag
     7501 ggcatccgcg gccgcttcga gcggtatgcg ttgggctcgc acctcggtgg cgaacaccga
     7561 ccaggaccct gccggactgt tctcgaagtc gcggcagcgc tgcgccgccc ggcgcggacg
     7621 gagccccggg gcgacgacgg gggaggctg
//
LOCUS       DAOSUG010000077         7635 bp    DNA     linear   ENV 10-JUN-2024
DEFINITION  MAG TPA_asm: Micropruina sp. isolate AMR_MDS_2195
            NODE_4841_length_7635_cov_4.374934, whole genome shotgun sequence.
ACCESSION   DAOSUG010000077 DAOSUG010000000
VERSION     DAOSUG010000077.1
DBLINK      BioProject: PRJNA925477
            BioSample: SAMN32864262
            Sequence Read Archive: SRR4236673
KEYWORDS    WGS; ENV; Metagenome Assembled Genome; MAG; Third Party Data; TPA;
            TPA:assembly.
SOURCE      Micropruina sp. (wastewater metagenome)
  ORGANISM  Micropruina sp.
            Bacteria; Bacillati; Actinomycetota; Actinomycetes;
            Propionibacteriales; Nocardioidaceae; Micropruina.
REFERENCE   1  (bases 1 to 7635)
  AUTHORS   Abdulkadir,N., Saraiva,J.P., Zhang,J., Stolte,S., Gillor,O.,
            Harms,H. and Rocha,U.
  TITLE     Genome-centric analyses of 165 metagenomes show that mobile genetic
            elements are crucial for the transmission of antimicrobial
            resistance genes to pathogens in activated sludge and wastewater
  JOURNAL   Microbiol Spectr 12 (3), e0291823 (2024)
   PUBMED   38289113
REFERENCE   2  (bases 1 to 7635)
  AUTHORS   Abdulkadir,N.
  TITLE     Direct Submission
  JOURNAL   Submitted (24-MAR-2023) Environmental Microbiology, Helmholtz
            Center for Environmental Research -UFZ, Permoserstr 15, Leipzig
            04318, Germany
COMMENT     The annotation was added by the NCBI Prokaryotic Genome Annotation
            Pipeline (PGAP). Information about PGAP can be found here:
            https://www.ncbi.nlm.nih.gov/genome/annotation_prok/
            
            ##Genome-Assembly-Data-START##
            Assembly Method        :: SPAdes v. 3.11.1
            Genome Representation  :: Full
            Expected Final Version :: Yes
            Genome Coverage        :: Not Applicable
            Sequencing Technology  :: Illumina MiSeq
            ##Genome-Assembly-Data-END##
            
            ##Genome-Annotation-Data-START##
            Annotation Provider               :: NCBI
            Annotation Date                   :: 05/04/2023 04:57:10
            Annotation Pipeline               :: NCBI Prokaryotic Genome
                                                 Annotation Pipeline (PGAP)
            Annotation Method                 :: Best-placed reference protein
                                                 set; GeneMarkS-2+
            Annotation Software revision      :: 6.5
            Features Annotated                :: Gene; CDS; rRNA; tRNA; ncRNA
            Genes (total)                     :: 2,776
            CDSs (total)                      :: 2,750
            Genes (coding)                    :: 2,714
            CDSs (with protein)               :: 2,714
            Genes (RNA)                       :: 26
            tRNAs                             :: 24
            ncRNAs                            :: 2
            Pseudo Genes (total)              :: 36
            CDSs (without protein)            :: 36
            Pseudo Genes (ambiguous residues) :: 0 of 36
            Pseudo Genes (frameshifted)       :: 2 of 36
            Pseudo Genes (incomplete)         :: 32 of 36
            Pseudo Genes (internal stop)      :: 2 of 36
            ##Genome-Annotation-Data-END##
FEATURES             Location/Qualifiers
     source          1..7635
                     /organism="Micropruina sp."
                     /mol_type="genomic DNA"
                     /submitter_seqid="NODE_4841_length_7635_cov_4.374934"
                     /isolate="AMR_MDS_2195"
                     /isolation_source="Activated sludge of municipal
                     wastewater treatment plants"
                     /db_xref="taxon:2737536"
                     /environmental_sample
                     /geo_loc_name="Canada"
                     /lat_lon="49.25 N 123.1 W"
                     /collection_date="2015-06-16"
                     /metagenome_source="wastewater metagenome"
                     /note="metagenomic"
     gene            <1..373
                     /locus_tag="PLF56_03900"
     CDS             <1..373
                     /locus_tag="PLF56_03900"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_013161215.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=2
                     /transl_table=11
                     /product="IclR family transcriptional regulator C-terminal
                     domain-containing protein"
                     /protein_id="HOQ52739.1"
                     /translation="SGLRDTIPVGSQLTMSAGSGAQVLLAWEDPERIQRGLLSSSFSA
                     VALATVRRRGWAQSVGEREAGVASVSAPVRSPSGKVIAAVSVSGPIERLSRQPGRLHA
                     PAVMAAAERLSDVLRRSGAEG"
     gene            complement(382..1311)
                     /locus_tag="PLF56_03905"
     CDS             complement(382..1311)
                     /locus_tag="PLF56_03905"
                     /inference="COORDINATES: protein motif:HMM:NF015679.2"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="L,D-transpeptidase family protein"
                     /protein_id="HOQ52740.1"
                     /translation="MRTAPLIAVAATLTMLAGCALTPAVAGPGAAPGAGASAASSPAV
                     TTPAATASTTATAGVTASTSPTPSPSPTKTTPPDPLAHLSAARRKAFKPCLSSTLKAG
                     SSGTCVSLLVKELKSAGFYPWSRTRYVTVAGANAILNYQRSRGLDADGWVGRDTWVAL
                     ATKAPTVSSKLPDTCYTKGVVLCVDQAHRKLTWLRNGKVVKTFKVRLGGYNQHAKTHI
                     WRNFPTANGTWKVYSKQVNPASETYGSGAMPYSTMFYPDMYVHYSAGFRSVGYAGSSH
                     GCVNIGKLSDAIWIFRNTPIGAKVHIYSPKATA"
     gene            complement(1633..2421)
                     /locus_tag="PLF56_03910"
     CDS             complement(1633..2421)
                     /locus_tag="PLF56_03910"
                     /inference="COORDINATES: protein motif:HMM:NF025054.2"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_function: GO:0008080 - N-acetyltransferase activity
                     [Evidence IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="GNAT family N-acetyltransferase"
                     /protein_id="HOQ52741.1"
                     /translation="MDIVTIQRLMARTWPGLEQERMGEWELRAAGGFTGRANSASPLG
                     DPGCPLDEALDRVEHWYRRRGLAPRLQVPASLDGSAGPSDEIGLLCARRGWTADPWTL
                     VMVRAARPDRIAPGHGTDEPALGDAAALDLRCASEPDQAWLDVYHYRGSQLPPTARRV
                     ITAAPAWYLTARLRGELVGIGRAAVAGDVVVLTAIEVVAAHRRRGFGAVITEALASRG
                     ADVGATLSALQVFAHNQPAQRLYRRLGYVDHHRYRYQCEAEPQI"
     gene            3452..4600
                     /locus_tag="PLF56_03915"
     CDS             3452..4600
                     /locus_tag="PLF56_03915"
                     /inference="COORDINATES: protein motif:HMM:NF017121.2"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="DUF726 domain-containing protein"
                     /protein_id="HOQ52742.1"
                     /translation="MVPRYCAEHRHEIPGFEKSAAAMHSIGDYESFLSFDSPNLSRAT
                     KIAGVAVVGAGVGAPAALLAAPAIGGAVGTLIGGYSGAVATNAGLAFLGGGSLAAGGL
                     GMAGGTMVVTAAGGALGGALGASVVNPYLRQDKSFRIEQLQSGDDIPVIVCNGFLSEK
                     GEGWGEWKQIVSRRYPHSPVYRVHWGAKELKDLGILASAAAIKTASVAAIKQAAAIAT
                     KAGAKKFGPLAPALLAMDLVKNPWHVAKNRADKTGAILADLIARTEADTYVLIGHSLG
                     ARAMVVATQTLSTKIDSRRVRELHLLGVAIGAQSDWQSLADGVDQAVYNYHSRNDSVL
                     KYIYRTAQAGQAAGGLGGLTPLTAGIHNVDVTDRVKSHFDYHANVTLF"
     gene            5002..5463
                     /locus_tag="PLF56_03920"
     CDS             5002..5463
                     /locus_tag="PLF56_03920"
                     /inference="COORDINATES: ab initio
                     prediction:GeneMarkS-2+"
                     /note="Derived by automated computational analysis using
                     gene prediction method: GeneMarkS-2+."
                     /codon_start=1
                     /transl_table=11
                     /product="hypothetical protein"
                     /protein_id="HOQ52743.1"
                     /translation="MRNDGLPLPEPDEVAVLHGYDLVRSDELPGIATRWLASGHPDSP
                     SVAELAASDPHDAWGAESLLKQIARDLSPQSAVQRTDDERVVIEWVTETWRATGDTWW
                     AVQTLARLGEEFPDFDLGVFVGYSDELAGGWGRLAPDLLQEAHAELGRRLA"
     gene            complement(5544..6074)
                     /locus_tag="PLF56_03925"
     CDS             complement(5544..6074)
                     /locus_tag="PLF56_03925"
                     /inference="COORDINATES: ab initio
                     prediction:GeneMarkS-2+"
                     /note="Derived by automated computational analysis using
                     gene prediction method: GeneMarkS-2+."
                     /codon_start=1
                     /transl_table=11
                     /product="hypothetical protein"
                     /protein_id="HOQ52744.1"
                     /translation="MANSDVDDPSTMALSEAESAYVAHFRAQVKQFGWVVSKAPDDWY
                     EGMETASANGRCLAWADVCVDDCVLLTVGAYFDGVTTTVGSLDSQTFDLRREDSRLST
                     TTFSGTLKEQAALAACWIDDVLRRGIRRREWSNTAKEYSFADDGTQLVHSGSRQDRAG
                     RPTRDTVIAGQAPGRD"
     gene            6221..6787
                     /locus_tag="PLF56_03930"
     CDS             6221..6787
                     /locus_tag="PLF56_03930"
                     /inference="COORDINATES: ab initio
                     prediction:GeneMarkS-2+"
                     /note="Derived by automated computational analysis using
                     gene prediction method: GeneMarkS-2+."
                     /codon_start=1
                     /transl_table=11
                     /product="hypothetical protein"
                     /protein_id="HOQ52745.1"
                     /translation="MIAEAFPSEKRRWLPAAEACLTVGPLPPVGCIHRSDREPLRVAG
                     ETLMIPHRQYDRPLDPASLEPHAKLVWLAWFTRSHDGRLRQAAIREFPRKPEPWLFPY
                     LIQPLGEYVIEISHDVRQLIAGALEDPPKAHALGKFWIANPSFAAITKSRARSYWDVY
                     YRPQAPTYAEYPAAMALAELDRNGAETH"
     gene            complement(6874..7284)
                     /locus_tag="PLF56_03935"
     CDS             complement(6874..7284)
                     /locus_tag="PLF56_03935"
                     /inference="COORDINATES: protein motif:HMM:NF026924.2"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="Imm8 family immunity protein"
                     /protein_id="HOQ52746.1"
                     /translation="MTLPVRGMSVRLMREDGGMRAALKSIGFQPEPSTLSADPAEFSF
                     LARLIIGPSDSPGEESFDVTVCTPEWLATASRRAGGIYDARHHVVVNLDDFNQPALQA
                     WLASRVHEAEAATWPELGQRLGRLGQWEFEGYQP"
BASE COUNT         1354 a         2490 c         2477 g         1314 t
ORIGIN      
        1 gtccggcctg cgcgacacca ttccggtcgg ctcgcaactg accatgtcgg ccggctccgg
       61 cgcccaggtg ctgctggcct gggaggatcc cgagcgcatc cagcgcggcc tgctgtcgtc
      121 gtcgttctcg gcggtggccc tggcaacggt gcggcggcgc gggtgggcgc aatcggtggg
      181 cgaacgcgaa gcgggcgttg cctcggtttc ggcaccggtg cgcagcccgt ccggcaaggt
      241 gatcgcggcg gtcagcgtct cgggcccgat cgaacgcctc tcacgccagc cgggtcgcct
      301 gcacgcccct gcggtgatgg ccgcggccga gcgcctcagc gacgtcttgc gccgctcggg
      361 cgccgagggc tgacggcggg tttaggccgt ggccttgggc gagtagatgt gcaccttggc
      421 gccgatcgga gtgtttctga agatccagat ggcgtcgctg agcttgccga tgttcacgca
      481 accgtggctt gaaccggcgt agccgacgct gcggaacccg gcggaatagt gcacatacat
      541 gtcggggtag aacatcgtgc tgtacggcat ggcgcctgag ccgtaggtct ccgaagccgg
      601 gttgacctgc ttgctgtaga ccttccaggt gccgttcgca gtggggaagt tgcgccagat
      661 gtgcgtcttc gcatgctggt tgtacccgcc gaggcgcacc ttgaaggtct tcaccacctt
      721 gccgttgcgc agccaggtga gcttgcggtg cgcctggtcg acgcagagca ccacgccctt
      781 ggtgtagcag gtgtcgggca gcttggacga gacggtgggc gccttcgtgg ccagcgccac
      841 ccaggtgtcc ctgcccaccc agccgtcggc gtcgagcccg cgcgagcgct ggtagttcaa
      901 gatggcgttc gccccagcga ccgtcacgta gcgggtgcgg ctccacggat agaagccggc
      961 cgacttgagt tccttgacca ggaggctgac gcaggtgccg gacgagccgg ccttcagcgt
     1021 gctcgacaga cacggcttga acgccttgcg gcgcgcggcg ctcaggtgcg ccagcgggtc
     1081 gggcggcgtg gttttcgtcg gggacgggct gggcgtcggg ctggtcgacg cagtgacgcc
     1141 ggcggttgcg gtggtcgagg cggtcgcagc gggggtcgtc acagccggtg atgacgccgc
     1201 cgacgctccc gcaccggggg ccgcaccggg gccggcgacg gcgggggtca gcgcgcatcc
     1261 ggcgagcatg gtcagggttg ccgccaccgc gatcagcgga gccgtacgca cccgaggagc
     1321 ggggctgggg tgctgatcgg tgtcacgggg agcggacgag aagtggcctg ttgtcaaccg
     1381 aacggtccct ccaaaacgac tactttcacc tcgtagccta cctggcgaag ccctcgaacc
     1441 cggcatcggc cccctgcacc accgtgagag tcacctggga atgggcacgc tgtgccggtg
     1501 ctgcgccgcc gtgcgtccat tggccaacgc tcagggtaac gcggatcaat cattggccga
     1561 gcgtgtggag accccttgtg agcaggggtt tctacgaact cacccaacgg aacgcgcgtg
     1621 gaccagcgct tctcagattt gaggctccgc ctcgcactgg tagcggtacc ggtgatggtc
     1681 gacgtacccg agccggcggt acagccgctg cgccggctgg ttgtgcgcga acacctgcag
     1741 cgcgctcagg gtcgccccca cgtctgcgcc gcgcgaggcc agcgcttcgg tgatcaccgc
     1801 cccgaagcca cgccgccggt gggcggcgac cacctcgatg gcggtcaaca ccaccacgtc
     1861 gcccgcgaca gcggcgcgtc cgatgcccac gagttcgccg cgcagccgcg cagtcagata
     1921 ccaggccgga gctgccgtga tcaccctccg cgccgtcggc ggcagttgcg acccgcggta
     1981 gtggtagacg tccagccacg cctggtcggg ttcgctcgcg caccgcaggt cgagcgcggc
     2041 cgcgtcaccg agcgccggct cgtcggtgcc gtgaccaggt gcgatccgat ccggacgagc
     2101 ggcgcgcacc atcaccaggg tccacggatc cgccgtccag ccgcgccgcg cacacaacag
     2161 cccgatctca tcggacggtc cggccgaccc gtccaggctc gccggcacct gcagccgcgg
     2221 tgccagcccg cgccgccggt accagtgctc gacacggtcg agggcctcat cgagcggaca
     2281 gcccggatca ccgaggggcg aagccgagtt ggcgcgtccg gtgaagccgc cggccgcgcg
     2341 gagttcccac tcccccattc gctcctgctc cagccctggc caggtgcgag ccatcagccg
     2401 ctggatggtc acgatgtcca cccacccagt gtggtgtagc cgctctatcg acgaagcagt
     2461 tcagttcgaa tccgcgccga gcatccgcat ctgctgctcc acccgagacc tcgatggagg
     2521 tgttggagtg tcctgacctg ccggcccagc cacgaacctg aacggtcagt gggtcggatg
     2581 ccgctcgtac gtcagcccga ccatcaaggt gctaccaccc accatctact cacttctgat
     2641 gaccgagctc gtcaaagtag gtacgcccgg atctgccgat gatctagctt gtcgagaggg
     2701 ccgtacgcgc gctcatgccg atgacagggt cgtcgagcgc gaccgaagtt ccatgagatg
     2761 cccgagatgt tcgagtctcc tcagcgagtt cacgcgtcgg atcgttggga tcaggggacg
     2821 caatcactat ggcccacgcc tgggctccgg acggccaggt ctggcccagc caatcctgag
     2881 cgcagcgagt gcctgttccg ctttcgtgat gtggcttcag aggcccaatc gagctacttg
     2941 gccgcttccc aggacttccg gtggcagagt tcgttgcatg gcgaagacga aggtggttgc
     3001 aaggttcttg gggaaccggc tcgagtgcat cgtcgaggca tcgcccgact tgggccttct
     3061 tttgtctggt aagccgaggc agctgtttcc gattgttgaa ggatcttctg cgcttgcaga
     3121 gaatcgcgcc ttagtcgaca acgcttggtc gtgtgcgctg tttacacatc aaggggtcac
     3181 cctgccaact cttgaagaac aggacgccgc gcacaagcga gtccgggccc accgcagcgt
     3241 tgttctagcg ctagccgaac ttgcggcaca cgcttcacaa gtgacgaagg acgggtggtg
     3301 ttccggctgc tttaccatgt cgactcaccg aaaagccaag acgccaaagg gccaactccc
     3361 tgcctacctg tgcagcaact gcgggtcccc cacgctgggc tgcgctgcca ggggatgccc
     3421 gtacatggcc acacggagcg gagcccaccc cgtggtgcct cgctattgcg cggagcatcg
     3481 gcacgaaatt cccggtttcg agaagagtgc tgccgcgatg cacagcatcg gtgactacga
     3541 gtcgtttctc agcttcgata gcccgaacct cagccgcgcg accaagattg ccggggtcgc
     3601 agtggtgggc gctggagtgg gcgcgcctgc ggcgctcctg gccgcaccgg caatcggcgg
     3661 agcggtcgga acactgatcg gcggctacag cggcgccgtc gcgaccaacg ccggcttggc
     3721 cttcctggga gggggatcac tggccgccgg cggtcttggg atggccggcg gcacaatggt
     3781 ggtcacggcg gccggaggtg ccctcggcgg cgccctgggc gcctccgtcg tcaaccccta
     3841 tctcaggcag gacaagtcat tccgcatcga gcaactccag tccggtgacg acatacccgt
     3901 gatcgtctgc aacggattcc tcagcgagaa aggcgaagga tggggcgaat ggaaacaaat
     3961 cgtaagtagg cgctacccac actcacccgt gtaccgcgtg cattggggcg ccaaagaact
     4021 caaagacctg ggtattctcg cgagcgcagc agcgatcaag acagcgagcg tggcggcgat
     4081 caagcaggcc gccgccatcg ccaccaaggc cggcgccaag aagttcggcc cgctagcccc
     4141 agcactgctc gcaatggacc ttgtgaaaaa cccctggcat gtggcaaaga accgcgccga
     4201 caagaccgga gcgatcctcg ccgatctcat cgcccgaacc gaagctgaca cgtatgtgct
     4261 gatcggccac agcctaggtg ctcgagcaat ggtggtggcc acccagactc tcagcaccaa
     4321 gatagacagt cgaagggtgc gcgaactaca cctactcggt gtcgccatcg gcgcccaaag
     4381 cgattggcag tcgctcgctg acggcgtcga ccaagcggtt tacaactacc actcacgcaa
     4441 cgactccgtc ttgaaataca tctaccgcac cgcccaagcg ggccaagcgg ccggtggact
     4501 cggcggctta accccactca cggccggaat ccacaacgtc gacgtgaccg accgagtgaa
     4561 gagccacttc gactaccacg caaacgtgac acttttctag ctgcaccacc agccatcgcg
     4621 cactggcgcg acgtactgtc atgccgcggt cagtgctctg aggttcggta gagtcaacgt
     4681 cattgagctg gtacatcaag gcgggcgatc gaacgtagaa ctccagcgat gtggtcggac
     4741 tcgtgccaag tacggacgag gcgcgcaact gggtcgatca taatccacca ccttcaggaa
     4801 tctgagtcgt tgctcggcca cgccgtgccc gaaggccgaa ggcaagccta aatccttgca
     4861 gagtcgggca tttctcgcag aggtgtaacg aggagaacac aaagtggcgc ggtcggccca
     4921 acgtccgctt ctgccgcgag ttctgcgtgc gtttgtacag gggtgggccg acttgtcgcg
     4981 aggatgatcc atgctgtgcg tgtgagaaac gacggactgc cgctgcccga accggatgaa
     5041 gtagcggtct tgcacggata cgacctggtc cgatcggatg agctgcccgg cattgccacg
     5101 cgttggctgg cgtccggaca cccggacagc ccgagcgtcg cagagctggc ggctagcgat
     5161 ccgcacgacg cgtggggagc cgagtcgctg ctgaagcaaa tcgcgaggga tcttagtccg
     5221 cagtctgccg tccagcgcac cgatgacgag cgggtcgtca tcgagtgggt gaccgaaacc
     5281 tggcgcgcga ccggtgacac ttggtgggca gttcagacat tggcccggct tggcgaggaa
     5341 ttcccagact tcgaccttgg cgtattcgtg ggctactccg acgagttggc aggaggatgg
     5401 ggccggctgg cgccagacct tctgcaggag gcgcatgcgg aattgggtcg caggctcgcc
     5461 tgaagtcgtc tctgggccac gcggacatct gcctacctcg cgcagggctg atcgtggccg
     5521 atgagggctt gtcgccgcac tgctcagtct cgtcccggcg cctggccggc gatcacagtg
     5581 tctcgggttg ggcgaccggc acggtcttgg cgggacccgg agtggacgag ctgtgtcccg
     5641 tcgtctgcga acgagtactc cttggctgtg tttgaccatt ctcgacgtcg tatcccgcgg
     5701 cgcagcacgt cgtctatcca gcaggcagca agggctgcct gttccttgag ggtgccagag
     5761 aaggtggtgg ttgacagccg ggaatcctca cgacgcaggt cgaacgtctg gctatcgagg
     5821 cttccaactg tggtcgtgac gccgtcgaag taggcgccca cggtcaggag cacgcagtcg
     5881 tcgacacaca cgtccgccca cgccagacac cttccgtttg cggatgctgt ctccatgcct
     5941 tcgtaccagt catcgggcgc ctttgagact acccatccga actgcttgac ctgtgctcga
     6001 aagtgcgcca cgtaggccga ctcggcctcg gacaaagcca tagtgctcgg atcgtcgaca
     6061 tcgctgttcg ccacgacgcc gagtttaggg tttcgcgttc ccaagcggcg cgcgaacgct
     6121 cgcagtaagc catccacctc cccccggatt gtcgcgatca cctgatgcgc gggctgcttt
     6181 ctggcagagt ggatctcctg cccaaatgga ggtggaacca gtgattgccg aagcattccc
     6241 gtccgagaaa cgccgttggc tgcccgcagc cgaggcatgc ctgacggtcg gtccgctacc
     6301 accagtcgga tgcatccaca gatccgaccg tgagccactc cgggtcgccg gtgagaccct
     6361 gatgattccc catcgtcagt acgaccgccc gctggaccca gcaagtctcg aaccccacgc
     6421 aaaacttgtc tggctcgcct ggttcacacg aagccacgat ggccggctcc gccaagccgc
     6481 gatacgcgag ttcccgagga aacctgaacc ttggctcttc ccgtacctga tccagccact
     6541 cggcgagtac gtcatcgaaa tctcgcacga cgtccgccag ctcatcgctg gcgccctcga
     6601 ggatccccca aaggcccatg cactcggcaa gttctggatc gccaacccga gcttcgcagc
     6661 catcacgaaa tcccgagccc gcagctactg ggacgtgtac taccggccgc aggctcccac
     6721 ctacgccgaa tatccagcag caatggcctt ggcggaactc gaccggaacg gtgcggaaac
     6781 gcactgatct gccgcggtgc gcgagggtag ctttcggggt tcgatgttcc gaagcacgac
     6841 cgccatctat tgcggcgcgg cgcgctagac gagtcagggc tggtagccct cgaactccca
     6901 ttgccctagg cgtccgagcc gctggccaag ctccggccag gtggccgcct ccgcttcgtg
     6961 aactcgggac gccagccaag cttggagggc gggctgattg aaatcgtcga ggttgaccac
     7021 aacgtggtgc ctcgcgtcgt agatgccgcc ggcccgacgg gaggcagtgg ccagccactc
     7081 cggcgtgcac acggtgacgt caaaggactc ttctccgggc gaatcagagg gaccaataat
     7141 gagccgcgcg aggaacgaga actcagcagg gtcagcagag agtgtggacg gctcgggttg
     7201 aaacccaatg cttttcagcg cagctcgcat tccaccatcc tcgcgcatca aacgcacgga
     7261 catgccgcgg accgggagtg tcattgtcag tcccttgcgc ccgcgggctt gacgtgccgc
     7321 catgctcggt gacgtgtgat ctgagagtat ctcgtgtctg ctgcgcagcg cttaagcgcg
     7381 ctggaaacct cggcactcca tactccctgg tcaacccagt caagcgcctt gcttgcccac
     7441 cagtccgacc aagaattcgc accgaacgct accaatcgag cagcgagcag ctcgtcgata
     7501 gaggcgtcgg agcggggatg ctctagcagc ggaaggaggg accgcagatc ctcgctggcg
     7561 tcacccacgc gagagaggtt aagcgcatcg cgtactttcc gcaattgccg ccgcgtgagc
     7621 atgatggtgg ggtgc
//
LOCUS       DAOSUG010000078         7551 bp    DNA     linear   ENV 10-JUN-2024
DEFINITION  MAG TPA_asm: Micropruina sp. isolate AMR_MDS_2195
            NODE_4881_length_7551_cov_3.943303, whole genome shotgun sequence.
ACCESSION   DAOSUG010000078 DAOSUG010000000
VERSION     DAOSUG010000078.1
DBLINK      BioProject: PRJNA925477
            BioSample: SAMN32864262
            Sequence Read Archive: SRR4236673
KEYWORDS    WGS; ENV; Metagenome Assembled Genome; MAG; Third Party Data; TPA;
            TPA:assembly.
SOURCE      Micropruina sp. (wastewater metagenome)
  ORGANISM  Micropruina sp.
            Bacteria; Bacillati; Actinomycetota; Actinomycetes;
            Propionibacteriales; Nocardioidaceae; Micropruina.
REFERENCE   1  (bases 1 to 7551)
  AUTHORS   Abdulkadir,N., Saraiva,J.P., Zhang,J., Stolte,S., Gillor,O.,
            Harms,H. and Rocha,U.
  TITLE     Genome-centric analyses of 165 metagenomes show that mobile genetic
            elements are crucial for the transmission of antimicrobial
            resistance genes to pathogens in activated sludge and wastewater
  JOURNAL   Microbiol Spectr 12 (3), e0291823 (2024)
   PUBMED   38289113
REFERENCE   2  (bases 1 to 7551)
  AUTHORS   Abdulkadir,N.
  TITLE     Direct Submission
  JOURNAL   Submitted (24-MAR-2023) Environmental Microbiology, Helmholtz
            Center for Environmental Research -UFZ, Permoserstr 15, Leipzig
            04318, Germany
COMMENT     The annotation was added by the NCBI Prokaryotic Genome Annotation
            Pipeline (PGAP). Information about PGAP can be found here:
            https://www.ncbi.nlm.nih.gov/genome/annotation_prok/
            
            ##Genome-Assembly-Data-START##
            Assembly Method        :: SPAdes v. 3.11.1
            Genome Representation  :: Full
            Expected Final Version :: Yes
            Genome Coverage        :: Not Applicable
            Sequencing Technology  :: Illumina MiSeq
            ##Genome-Assembly-Data-END##
            
            ##Genome-Annotation-Data-START##
            Annotation Provider               :: NCBI
            Annotation Date                   :: 05/04/2023 04:57:10
            Annotation Pipeline               :: NCBI Prokaryotic Genome
                                                 Annotation Pipeline (PGAP)
            Annotation Method                 :: Best-placed reference protein
                                                 set; GeneMarkS-2+
            Annotation Software revision      :: 6.5
            Features Annotated                :: Gene; CDS; rRNA; tRNA; ncRNA
            Genes (total)                     :: 2,776
            CDSs (total)                      :: 2,750
            Genes (coding)                    :: 2,714
            CDSs (with protein)               :: 2,714
            Genes (RNA)                       :: 26
            tRNAs                             :: 24
            ncRNAs                            :: 2
            Pseudo Genes (total)              :: 36
            CDSs (without protein)            :: 36
            Pseudo Genes (ambiguous residues) :: 0 of 36
            Pseudo Genes (frameshifted)       :: 2 of 36
            Pseudo Genes (incomplete)         :: 32 of 36
            Pseudo Genes (internal stop)      :: 2 of 36
            ##Genome-Annotation-Data-END##
FEATURES             Location/Qualifiers
     source          1..7551
                     /organism="Micropruina sp."
                     /mol_type="genomic DNA"
                     /submitter_seqid="NODE_4881_length_7551_cov_3.943303"
                     /isolate="AMR_MDS_2195"
                     /isolation_source="Activated sludge of municipal
                     wastewater treatment plants"
                     /db_xref="taxon:2737536"
                     /environmental_sample
                     /geo_loc_name="Canada"
                     /lat_lon="49.25 N 123.1 W"
                     /collection_date="2015-06-16"
                     /metagenome_source="wastewater metagenome"
                     /note="metagenomic"
     gene            complement(33..503)
                     /locus_tag="PLF56_03940"
     CDS             complement(33..503)
                     /locus_tag="PLF56_03940"
                     /EC_number="2.3.1.-"
                     /inference="COORDINATES: protein motif:HMM:NF025054.2"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_function: GO:0008080 - N-acetyltransferase activity
                     [Evidence IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="GNAT family N-acetyltransferase"
                     /protein_id="HOQ52747.1"
                     /translation="MSGRTVLIDRATPSDLPAILALEQTGFLPASRWVDKSWLPELGG
                     GHRFAPAVRDDDGMLRGIAAFQLIAEVADLNRVVVDPGFRGRGMGRALLEAGLAWAAS
                     AGGERMLLEVEWANEPALALYRGMGFVEISYRANYYGAGRDALVMQRELNEWGS"
     gene            complement(500..1171)
                     /gene="tsaB"
                     /locus_tag="PLF56_03945"
     CDS             complement(500..1171)
                     /gene="tsaB"
                     /locus_tag="PLF56_03945"
                     /EC_number="2.3.1.234"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_004808954.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_function: GO:0003725 - double-stranded RNA binding
                     [Evidence IEA];
                     GO_process: GO:0006400 - tRNA modification [Evidence IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="tRNA
                     (adenosine(37)-N6)-threonylcarbamoyltransferase complex
                     dimerization subunit type 1 TsaB"
                     /protein_id="HOQ52748.1"
                     /translation="MSLTLAIDTSTDVRVGLAAHGAVLASGAVTDPRAHAERLMPLIH
                     ETLHNAARTLADVTQLVVGVGPGPFTGLRVGVVTAQTLGFALGVPVRGVCSLDVGAVG
                     YAGHADAAGEYLADAAGEFLVVSDARRKEVYWARYGADASRLTGPFVGPAAELPDLPV
                     TGPGAELAGGRSTPLDGGLLALAGPDLPDVGLSPLYLRRPDAEVPTTRKSTLLQPRLA
                     PGLKR"
     gene            complement(1210..1638)
                     /gene="tsaE"
                     /locus_tag="PLF56_03950"
                     /pseudo
     CDS             complement(1210..1638)
                     /gene="tsaE"
                     /locus_tag="PLF56_03950"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_002520619.1"
                     /note="incomplete; partial in the middle of a contig;
                     missing N-terminus and C-terminus; Derived by automated
                     computational analysis using gene prediction method:
                     Protein Homology.
                     GO_component: GO:0005737 - cytoplasm [Evidence IEA];
                     GO_function: GO:0016887 - ATP hydrolysis activity
                     [Evidence IEA];
                     GO_process: GO:0006400 - tRNA modification [Evidence IEA]"
                     /pseudo
                     /codon_start=1
                     /transl_table=11
                     /product="tRNA
                     (adenosine(37)-N6)-threonylcarbamoyltransferase complex
                     ATPase subunit type 1 TsaE"
     gene            complement(1750..2112)
                     /locus_tag="PLF56_03955"
                     /pseudo
     CDS             complement(1750..2112)
                     /locus_tag="PLF56_03955"
                     /inference="COORDINATES: protein motif:HMM:NF012792.2"
                     /note="incomplete; partial in the middle of a contig;
                     missing C-terminus; Derived by automated computational
                     analysis using gene prediction method: Protein Homology.
                     GO_function: GO:0008080 - N-acetyltransferase activity
                     [Evidence IEA]"
                     /pseudo
                     /codon_start=1
                     /transl_table=11
                     /product="GNAT family N-acetyltransferase"
     gene            complement(2679..>3795)
                     /locus_tag="PLF56_03960"
     CDS             complement(2679..>3795)
                     /locus_tag="PLF56_03960"
                     /EC_number="1.1.1.17"
                     /inference="COORDINATES: protein motif:HMM:NF002652.0"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_function: GO:0008926 - mannitol-1-phosphate
                     5-dehydrogenase activity [Evidence IEA];
                     GO_process: GO:0019594 - mannitol metabolic process
                     [Evidence IEA]"
                     /codon_start=2
                     /transl_table=11
                     /product="mannitol-1-phosphate 5-dehydrogenase"
                     /protein_id="HOQ52749.1"
                     /translation="ERAAAEYVATELSNAGYELVFADVAEALIDALANSGSYAVHEVG
                     DDPRTTVVRGYSALNSAKQAEELTAAIASADIVTTAVGAHILKFVAPAIAAGIAARPD
                     GAPKVAVMACENAINGTDILRGEVELAYAGDDLADRALFANTAVDRIVPAQDPNAGLD
                     VTVENYFEWAIETPPFGGRVPEIPGVTWVDDLEPYIERKLFTVNTGHATAAWFGHAAG
                     IEKISDALAEPSVAAKVADALADTKSLIVAKHGVDETVQTAYVSKILKRFANPHLPDT
                     TLRVGRGPLRKLSRHDRFISPAAQLADRGMPANGLLAAIGAGLSFDTPDDPEAVELQR
                     LLGELSPEQAVVQITGLDSDHPLYPAVLEVFQRHQQG"
     assembly_gap    3796..3805
                     /estimated_length=10
                     /gap_type="within scaffold"
                     /linkage_evidence="paired-ends"
     gene            complement(3943..6075)
                     /locus_tag="PLF56_03965"
     CDS             complement(3943..6075)
                     /locus_tag="PLF56_03965"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_011756600.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="PTS sugar transporter subunit IIA"
                     /protein_id="HOQ52750.1"
                     /translation="MSTTTAAPKSSARIHVQKFGTFLSGMIMPNIPALIAWGILTMFF
                     IPAGFTPNASVATIVGPMIHYLLPIIIAYTGGHMVYGLRGAVVASIGVMGAIAGSDYL
                     IAQENVRLLEAWLAAGNAEADFKELGQVHMFIGAMIMAPVSAYSMRWLDKLWENKIKA
                     GLEMLVNMFSAGIWAFVLMIIGFFPVAWLVNGLMSILSSAVNWLVGHNLLPLTSIVIE
                     PAKVLFLNNAINHGVLTPLGLAQAGDAAYNNRSILFLLESNPGPGLGLLLAFAVVGVG
                     AAKASAPGAAFIQFVGGIHEVYFPYALMKPTLILALIGGGMTGVTTNMLLGGALRAPA
                     APGSILAVIAQTAPGAYFAVILSVILSATVTFLIAGVILRSSRKADLEAEAAGEDKFA
                     SAISQTEANKGKSSDALSGLLGAGASAPAGKVKSIVFACDAGMGSSAMGASVLRKKIQ
                     SAGFGDVTVVNKAISSLSDTFDVVVSHEDLMDRASQRTPSAVHVAVDNFMSSPRYDEV
                     VEMVRRVNSDAPAEAAPASASATPAPAAPAQPAAPAQPAAPAPAAGTPATEQRADSET
                     SDSVLKLTSIVLEGSATDRNAAISEAGDLLVASGNVDAAYVASMHEREKSVSTYMGNL
                     LAIPHGTNEAKSLISSSSISVVRYPGGIDWNGNPVKFVIGIAGAGNDHLELLGKIAEV
                     FLDESKIAELEAATSPQQIKDAFGKVNG"
     gene            complement(6401..7393)
                     /locus_tag="PLF56_03970"
     CDS             complement(6401..7393)
                     /locus_tag="PLF56_03970"
                     /inference="COORDINATES: protein motif:HMM:NF013425.2"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_function: GO:0052855 - ADP-dependent NAD(P)H-hydrate
                     dehydratase activity [Evidence IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="NAD(P)H-hydrate dehydratase"
                     /protein_id="HOQ52751.1"
                     /translation="MPTEVSTSSTAGAESSTGDGVSTNSTAGASHFTASLTVTFGGLK
                     FCHLLEPAASACGRVELIDIGLALPMPDLRAWQPVDLAAAWPFPDATSDKYSRGVVGI
                     DTGSNEYPGAAVLGTLGAVHAGAGMVRFTGAEGAVDLIRTEAPNVVIGLGRVQARVLG
                     SGWGDRTDGRATVRQALDEGLPLVLDADALRFLPGGPLGHLVLLTPHAGELARLLGVD
                     RPQVQADPLGSVRAASARVGATVLLKGATQYVCGPDGEISIAVPGPAWTAQAGSGDVL
                     AGICGTLLAAGLEPAAAALAAASVQSLAARNSPGPHPPQDVARALARVIASLRA"
BASE COUNT         1228 a         2570 c         2679 g         1064 t
ORIGIN      
        1 cgatacccaa caccaacgga ccgctcacag cgtcaactcc cccactcgtt cagctcgcgc
       61 tgcatcacca gcgcgtcgcg tccggcaccg tagtagttgg cgcggtagct gatctcgacg
      121 aaacccatgc cccggtacag cgccagggcc ggctcattgg cccactcgac ctccagcagc
      181 atgcgttcgc cgcccgcgga cgccgcccac gccagcccgg cttcgagcag cgcccggccc
      241 atgccccgtc cgcgaaagcc gggatcgacc accacccggt tcaggtcggc cacctcggcg
      301 atcaactgga aggcggcgat gccccgcagc atcccgtcgt cgtcacgcac cgcgggagcg
      361 aaccgatgcc cgccgcccag ctcgggcagc catgacttgt cgacccatcg ggacgccggc
      421 agaaaaccgg tctgttccag agcgaggatc gcgggcaggt cggacggggt ggcccggtcg
      481 atcaacacgg tgcgaccgct catcgcttca ggcccggcgc cagccggggt tgcagcaggg
      541 tcgacttgcg agtggtcggc acctcggcgt ccggacggcg cagatacagc ggcgagaggc
      601 ccacatcggg caggtcgggg ccggccaggg cgagcagtcc accgtccagc ggtgtggacc
      661 tgccgccggc cagttctgcc cccgggccgg tgacgggcag gtcgggcaac tcggcggccg
      721 gaccgacgaa aggaccggtg aggcggctcg cgtccgcgcc gtagcgagcc cagtagacct
      781 ctttgcggcg ggcgtccgag accaccagaa actcaccggc cgcgtccgca agatattcac
      841 cggccgcgtc cgcatgaccg gcgtagccga ccgcaccgac gtccagcgag cacacgccac
      901 gaaccggcac gccgagcgcg aaacccaggg tctgcgcggt gacgaccccg acccgcaggc
      961 cggtgaacgg acccggcccg acaccgacca ccaactgcgt gacgtcggcg agcgtgcgcg
     1021 cggcattgtg cagggtctcg tggatcagcg gcatcagtcg ctccgcgtgt gcccgcggat
     1081 cggtgaccgc gcccgaggcc agcacggcac cgtgcgcggc caggccgacg cgcacgtcgg
     1141 tcgaggtgtc gatcgcgagg gtcaggctca tcggggtgcc tccacgtgtc gcagcgtctg
     1201 tagcggcgca tcggcccagc gcgggccacg cgcggccaga aacaccagcc gggtgtcgtc
     1261 gtcggggtcg agaccccggc gaatgtcgat gtcgagccgg ttctcggcca ggccctcggc
     1321 gatgccgccg ccccattcga tcagggtgac cgagtcggcc agggacgcct ccaggtcaag
     1381 gtcttcgagt tcgtcgaaac tgcccagccg gtaggcgtcg acgtggacca gcgccgggcc
     1441 gtccgtggtg ctggggtgca cgcgcgagag cacgaaggtg ggcgagatga ccgggccgct
     1501 gaccgccagg cccgccccga tgccctgggt cagcgtggtc ttgcctgcgc ccaggtcgcc
     1561 ggtcgcgatc agcacgtcac cgggggtgag cagggcggcg aggcgccgtc cgagttcgcg
     1621 catcgactcg gccgtcggcg cttcgacgag gatcggaacg gtgagttgcc aggtggtggc
     1681 gccgggtacc ggcacgaacc cgtggcggtg cagccagtgc ggcgcccagc cgggtgcgtc
     1741 cgtcggatcc agttgaagcg ttcgcacgcc gcgtgcggcc aacaggtccg tcgtggtctg
     1801 gacgagcgcg gcggcgaccc cccgtccgcg ctgcccaggg tgcacggcga ggcccgccag
     1861 tcgggctgcc gcgccgtcga gcatgctgag cactgcggcg atcggggccc cgccgacctc
     1921 ggccaccagg cccagcgccg tctcggacga tgcggcggca gccgcgctga tgccgcaggg
     1981 gcgagtgccg ctcaggctct cgagcagtgc tgccgcgcgg gcctgtggca cctggacgat
     2041 gcgcagcgtg ccgccgtcgg ggaaggcggc ctcggcgaca acgtcgaagc ctgatccgga
     2101 cggggcggac atgcgaaacc tccaggaagc gtcggtggga gccgtccgag tctagtccag
     2161 ggcgagtggg cccggattcg cgggggccgc cgatgtgcgc ggcccacgcc cggccgccgt
     2221 gatgtgggcc gccacgctca ccaagtcgac gacctgccct cgagcgggcc ggcaaggggc
     2281 cccgcggcaa cggccagagc gccgactcag ttggtcacgt gtgaacgggt gggttttgat
     2341 acagcagtcg gtgctgaaac ccacccgtag cgcggaacca ccaatggccg gcgtcgcgag
     2401 cggcagcggc cgggcacatc ggtccgcgcg acggcatgcc ggtcgtctgt tgagcgtggc
     2461 cggcgaccac cagggagccc ggatggggtc gcacagcagg cggcgatcgg tagatcgggg
     2521 ctggcccgga cccaggctga tccatcgctt ggtgcgaccc cagtcagggg cggtttccac
     2581 atggtcagcc aacggatcgc tcaagaaatg cgcacctcta cgcaacgccg acacggcgcc
     2641 ccgggagacc cgggacgccg tgtggaaccg tctgagcgtc agccctgctg gtgccgctga
     2701 aacacctcca gcacggccgg gtacagcgga tggtcggaat cgagtccggt gatctgcacg
     2761 accgcctgct cgggcgacaa ctcgcccagc agacgctgca actcgaccgc ctcgggatca
     2821 tcgggggtgt cgaagctcag ccccgccccg atcgccgcca gcagcccgtt cgccggcatg
     2881 ccgcggtcgg ccagctgtgc ggccggcgag atgaaccggt cgtgacggct gagcttgcgc
     2941 agcggtccgc ggccgacccg cagggtggtg tcgggcaggt gcgggttggc gaaccgcttg
     3001 aggatcttcg acacgtacgc ggtctgcacc gtctcgtcca cgccgtgctt ggccacgatc
     3061 agcgacttgg tgtcggccag cgcgtccgcg accttggcgg cgacggacgg ttcggccagg
     3121 gcgtccgaga tcttctcgat gcctgccgcg tgaccgaacc aggccgcggt ggcgtggccg
     3181 gtgttgaccg tgaagagctt gcgctcgatg tagggctcga ggtcatcgac ccaggtcacg
     3241 cccggaatct cgggcacccg gcccccgaac ggcggggtct cgatcgccca ctcgaagtag
     3301 ttctcgaccg tgacgtccag gcccgcgttg ggatcctggg ccggcacgat ccgatcaacc
     3361 gcggtgttgg cgaacagcgc ccggtcggcc aggtcgtcgc cggcgtacgc caactcgacc
     3421 tcgccgcgca gaatgtcggt gccgttgatg gcgttctcgc acgccatcac cgccaccttg
     3481 ggcgccccgt cggggcgggc cgcgatcccg gccgcgatgg ccggggccac gaacttgaga
     3541 atgtgcgcgc cgaccgccgt ggtgacgatg tcggcggacg cgatcgcggc ggtcagctcc
     3601 tcggcctgct tggccgagtt gagagcggag tacccgcgca ccacggtggt gcgcgggtca
     3661 tcacccacct cgtgcacggc gtaggagccg gagttcgcca gggcgtcgat gagcgcctcg
     3721 gcaacgtctg cgaacaccag ttcgtagccg gcgttgctca gctcggtcgc gacgtactcg
     3781 gcggcggcgc gctccnnnnn nnnnnactcc ggcgccgcgt tgatggcgtc gacgaggtcg
     3841 gaggcgacat cggagaacac caggtcgtag ccggcctcgt gcagcagcag accgacgaac
     3901 cctcgtccga tgttgcccgc gccgaagtgg accgctttca tgtcagccgt tcacctttcc
     3961 gaaggcgtcc ttgatctgct gcgggctggt ggccgcctcg agttcggcga tcttggactc
     4021 gtccaggaac acctccgcga tcttgcccag cagctccagg tggtcgttgc ccgcgccggc
     4081 gatgccgatc acgaacttga ccgggttgcc gttccagtcg atgccgcccg ggtagcgcac
     4141 caccgagatc gacgagctcg agatcagcga cttggcctcg ttggtgccgt gcggaatggc
     4201 cagcaggttg cccatgtagg tcgacaccga cttctcgcgc tcgtgcatcg aggcgacgta
     4261 ggccgcatcg acgttgccgg aggccaccag caggtcgccg gcctcgctga tcgcagcgtt
     4321 gcggtcggtc gccgagcctt cgagcacgat cgaggtcaac ttcaacaccg aatcggacgt
     4381 ctctgagtca gctcgctgct cggtggccgg ggtgcctgcg gccggagcgg gagcagccgg
     4441 ttgagcggga gcggccggtt gagcgggagc ggcgggagca ggagtggccg aagccgaagc
     4501 cggagcggcc tcggccggag cgtcggagtt gacccgacgc accatctcga ccacctcgtc
     4561 gtagcgtggc gacgacatga agttgtcgac cgccacgtgc accgccgacg gggttcgctg
     4621 cgaggcccgg tccatcaggt cctcatgcga gaccaccacg tcgaaggtgt cggacaggct
     4681 cgaaatcgcc ttgttgacca ccgtgacatc gccgaacccg gccgactgaa tcttcttgcg
     4741 gagcaccgag gcacccatgg cggacgagcc catgcccgcg tcgcacgcga acacgatcga
     4801 cttgaccttg ccggccgggg cggatgcgcc cgcaccgagc aggcccgaaa gggcgtcgga
     4861 cgacttgccc ttgttggcct cggtctgact gatcgcactg gcgaacttgt cttcgccggc
     4921 cgcctcggcc tcgagatccg ccttgcggga cgaccgcagg atcaccccgg cgatcaggaa
     4981 cgtgaccgtt gccgacagga tcaccgacaa gatgacggcg aagtaggcac ccggagcggt
     5041 ctgggcgatg acagcgagga tgctgcccgg agccgccggg gcacgcaatg cgccacccaa
     5101 cagcatgttg gtggtgacgc ccgtcatgcc gccgccgatc agggcgagga tgagggtcgg
     5161 cttcatcagc gcgtagggga agtagacctc gtgaataccg ccgacgaact ggatgaaggc
     5221 ggcgcccgga gccgacgcct tggcggcacc gacaccgacc acggcgaagg ccagcagcag
     5281 cccgagaccc gggccggggt tggattcgag caggaacagg atggaccggt tgttgtaagc
     5341 ggcgtcgccg gcctgggcca ggcccaacgg ggtgagtacg ccgtggttga tcgcgttgtt
     5401 gaggaacagc accttggccg gttcgatcac gatgctggtg agcggcagca ggttgtggcc
     5461 gaccagccag ttgacggcac tgctcagtat cgacatcagg ccgttgacca gccacgcaac
     5521 ggggaagaag ccgatgatca tcagcacgaa ggcccagatg ccggccgaga acatgttgac
     5581 cagcatttcg aggccggcct tgatcttgtt ctcccacagc ttgtcgagcc agcgcatcga
     5641 ataggccgac accggggcca tgatcatggc gccgatgaac atgtgcacct ggccgagttc
     5701 cttgaaatcg gcttcggcgt tgcctgcggc cagccaggcc tcgagcagcc gcacgttctc
     5761 ttgggcgatc aggtagtcgg agccggcgat cgcacccatc acaccgatcg atgcaacgac
     5821 cgccccgcgc agcccgtaga ccatgtgtcc gccggtgtag gcgatgatga tcggcagcag
     5881 gtagtggatc atcgggccga cgatggtcgc gacgctcgca ttcggcgtga agcccgccgg
     5941 aatgaagaac atggtgagaa tgccccacgc gatcagcgcg gggatgttgg gcatgatcat
     6001 gcccgacaag aacgtgccga acttctgcac gtggatgcgg gccgaggact tcggcgccgc
     6061 ggtggtggtc gacatagatc tcctcattgg tcgtgcccag tgcggagccg gccagcgtcg
     6121 gacgtgcccg cgtgtgagca actctgtgat gcacactaga ccggacaaat catcgacagc
     6181 caccaacagg ccgtactgag tctgcagatc gaccggcggc gccgggtatc gagtaccgct
     6241 gagcctggct ccaccgatgt gccccgtcgc gagcggcacg gccgggtggc ctggcaagac
     6301 ggtccgcgtg aaggcatacc gggcgtctgg tgaagcgtgg ccgacgcagc cagggtgccc
     6361 ggatggggcc gtgccgtagg cgtagatcgg tggatccggg ctaagcgcgc agggacgcta
     6421 tcacccgcgc aagcgcccgg gcaacatcct gcggaggatg tggcccgggg ctgttccggg
     6481 cggcgagcga ctgcaccgag gccgcagcta gtgccgccgc cgcgggttcc agaccggcgg
     6541 ccagcagtgt gccgcagatg ccggccagca cgtcgcccga cccggcctgg gccgtccacg
     6601 ccggcccggg tacagcgatc gagatttctc catccggccc acagacgtac tgggtggcgc
     6661 ccttgagcag cacggtggcg ccaaccctgg ccgaggccgc gcggacggac ccgagcgggt
     6721 cggcctgcac ctgcgggcga tcgacgccga gcaaccgggc cagctcgccg gcgtgcgggg
     6781 tgagcagcac cagatgcccg agcgggccgc cgggaagaaa gcgtagtgcg tcggcgtcca
     6841 gcaccagggg cagcccctcg tccagtgcct gtcggacggt ggcgcgcccg tcggttcgat
     6901 cgccccagcc gctgccgagc acccgggcct gcacgcgtcc cagaccgatc acgacgttcg
     6961 gcgcctcggt gcggatcagg tcgaccgccc cctcggcacc ggtgaaccgg accatgcccg
     7021 caccggcgtg gacggcgccc agggtgccca gcacagcggc gcccgggtac tcgttcgagc
     7081 cggtgtcgat gccgaccacg ccccgggagt acttgtcgct cgttgcgtcc ggaaagggcc
     7141 aggctgcggc gaggtccacc ggctgccagg cccgcaggtc gggcatcgga agagccagac
     7201 cgatgtcgat caactcgacc cggccgcagg ccgaggcggc gggttcaagg aggtggcaga
     7261 acttgagccc gccgaaggtc acggtcagcg acgcggtgaa gtgggacgct ccggcggtcg
     7321 agttcgtcga aaccccatcg ccggtcgacg actccgcacc agcagtcgag ctggtcgaga
     7381 cctcagtggg catggtcgaa tggaacgcaa ccgtcatccc ggcggacgcc gggatctcag
     7441 ccgacacgtc cgcaccctgg gcaccgaacg cgggcgcctc cccaagatcc cggctttcgc
     7501 cgggatgacg aagggggccg ggatgacgaa gggggccggg atgacgaagg g
//
LOCUS       DAOSUG010000079         7494 bp    DNA     linear   ENV 10-JUN-2024
DEFINITION  MAG TPA_asm: Micropruina sp. isolate AMR_MDS_2195
            NODE_4915_length_7494_cov_3.463638, whole genome shotgun sequence.
ACCESSION   DAOSUG010000079 DAOSUG010000000
VERSION     DAOSUG010000079.1
DBLINK      BioProject: PRJNA925477
            BioSample: SAMN32864262
            Sequence Read Archive: SRR4236673
KEYWORDS    WGS; ENV; Metagenome Assembled Genome; MAG; Third Party Data; TPA;
            TPA:assembly.
SOURCE      Micropruina sp. (wastewater metagenome)
  ORGANISM  Micropruina sp.
            Bacteria; Bacillati; Actinomycetota; Actinomycetes;
            Propionibacteriales; Nocardioidaceae; Micropruina.
REFERENCE   1  (bases 1 to 7494)
  AUTHORS   Abdulkadir,N., Saraiva,J.P., Zhang,J., Stolte,S., Gillor,O.,
            Harms,H. and Rocha,U.
  TITLE     Genome-centric analyses of 165 metagenomes show that mobile genetic
            elements are crucial for the transmission of antimicrobial
            resistance genes to pathogens in activated sludge and wastewater
  JOURNAL   Microbiol Spectr 12 (3), e0291823 (2024)
   PUBMED   38289113
REFERENCE   2  (bases 1 to 7494)
  AUTHORS   Abdulkadir,N.
  TITLE     Direct Submission
  JOURNAL   Submitted (24-MAR-2023) Environmental Microbiology, Helmholtz
            Center for Environmental Research -UFZ, Permoserstr 15, Leipzig
            04318, Germany
COMMENT     The annotation was added by the NCBI Prokaryotic Genome Annotation
            Pipeline (PGAP). Information about PGAP can be found here:
            https://www.ncbi.nlm.nih.gov/genome/annotation_prok/
            
            ##Genome-Assembly-Data-START##
            Assembly Method        :: SPAdes v. 3.11.1
            Genome Representation  :: Full
            Expected Final Version :: Yes
            Genome Coverage        :: Not Applicable
            Sequencing Technology  :: Illumina MiSeq
            ##Genome-Assembly-Data-END##
            
            ##Genome-Annotation-Data-START##
            Annotation Provider               :: NCBI
            Annotation Date                   :: 05/04/2023 04:57:10
            Annotation Pipeline               :: NCBI Prokaryotic Genome
                                                 Annotation Pipeline (PGAP)
            Annotation Method                 :: Best-placed reference protein
                                                 set; GeneMarkS-2+
            Annotation Software revision      :: 6.5
            Features Annotated                :: Gene; CDS; rRNA; tRNA; ncRNA
            Genes (total)                     :: 2,776
            CDSs (total)                      :: 2,750
            Genes (coding)                    :: 2,714
            CDSs (with protein)               :: 2,714
            Genes (RNA)                       :: 26
            tRNAs                             :: 24
            ncRNAs                            :: 2
            Pseudo Genes (total)              :: 36
            CDSs (without protein)            :: 36
            Pseudo Genes (ambiguous residues) :: 0 of 36
            Pseudo Genes (frameshifted)       :: 2 of 36
            Pseudo Genes (incomplete)         :: 32 of 36
            Pseudo Genes (internal stop)      :: 2 of 36
            ##Genome-Annotation-Data-END##
FEATURES             Location/Qualifiers
     source          1..7494
                     /organism="Micropruina sp."
                     /mol_type="genomic DNA"
                     /submitter_seqid="NODE_4915_length_7494_cov_3.463638"
                     /isolate="AMR_MDS_2195"
                     /isolation_source="Activated sludge of municipal
                     wastewater treatment plants"
                     /db_xref="taxon:2737536"
                     /environmental_sample
                     /geo_loc_name="Canada"
                     /lat_lon="49.25 N 123.1 W"
                     /collection_date="2015-06-16"
                     /metagenome_source="wastewater metagenome"
                     /note="metagenomic"
     gene            <1..1115
                     /locus_tag="PLF56_03975"
     CDS             <1..1115
                     /locus_tag="PLF56_03975"
                     /inference="COORDINATES: protein motif:HMM:NF016959.2"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=3
                     /transl_table=11
                     /product="DivIVA domain-containing protein"
                     /protein_id="HOQ52752.1"
                     /translation="DLFDETASAAGSFPHSMLGYERQAVDAYIRDIERQLSSAKRKVR
                     SLQRQVATKETETDLSKLGDHARDVLRAAEAEAAELRNQARLDADRIVAQAAAEAEEV
                     AHNTQAETSTHRVANLDDLNRLREELANQTGAELNAARQEAQLLRDAAENHRRMVLAD
                     AERNATALREQAALEAEQVRQAAQKEAADLRAAIAAEHAQALDALQRHQYEVTQHVGQ
                     LSEQARLQSDQFGADMAHQVELMNNRRNVAASEADKIVADADDRARAIIAEARAAAAK
                     REREAGLAEADKISKLNEEIARLRVRHGELQKILRSLSQMATSSFDDEPAAAAQAPAS
                     AAPEPQGQPAAEAAQPNLDDDATRVQPAASGGSNRR"
     gene            1121..2362
                     /locus_tag="PLF56_03980"
     CDS             1121..2362
                     /locus_tag="PLF56_03980"
                     /inference="COORDINATES: protein motif:HMM:NF013740.2"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="AI-2E family transporter"
                     /protein_id="HOQ52753.1"
                     /translation="MTAQPNEVGGDENSVADGTVPTAGPSAAPSAAQPTPSRPRGWLH
                     RIRRATQPTPPDLDPDAIHAGPRNEISMAQRSPFAVGFFITLGALTAFGLVFGLVRIA
                     SVLLLVMLALYLALGMNPLVERLSSRGLRRGLAVLLVALGALAVLALAFWAVLPVAYE
                     QINRLLLNAPALLQELRANPQIAQLDARYDVINRVSAFLTSGELLSTLFGGLWGASQA
                     VAGLVFSTMITLTLTLYILASMPTFKDAIFELAPASRRARVRYLANEMFGRIGGYVTG
                     LFIIITCAATVAFLFLSIVGLGQYALALAVVVALCAVIPLIGSTLSMIIVSIVAFTFS
                     ATQGLITLAFFLTYQQIDNYFIQPRVFQRSVSVPGPLVILAAVCGGLLYGLLGALLAI
                     PSMAALMLLYREVLIPALDRR"
     gene            complement(2865..3548)
                     /gene="nucS"
                     /locus_tag="PLF56_03985"
     CDS             complement(2865..3548)
                     /gene="nucS"
                     /locus_tag="PLF56_03985"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_013863881.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_function: GO:0004519 - endonuclease activity [Evidence
                     IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="endonuclease NucS"
                     /protein_id="HOQ52754.1"
                     /translation="MRLIVARCQVDYAGRLSAHLPLATRLILAKADGSVSIHADDRAY
                     KPLNWMNPPCTLTVTTAEADSGFSEVWELRNKDADTLVISIAEVLHDSHHELGVDPGL
                     VKDGVEAHLQVLLAEHPEAFGPGWTLVQREYLTPIGPVDLLFRDDAGGYVAVEVKRRG
                     EIDGVEQLTRYLALMNADPLLAPVKGVFAAQLIKPQAKTLAGTRGITCVSVDYDALRG
                     LDNAEDRLF"
     gene            3632..3904
                     /locus_tag="PLF56_03990"
     CDS             3632..3904
                     /locus_tag="PLF56_03990"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_013863882.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="hypothetical protein"
                     /protein_id="HOQ52755.1"
                     /translation="MPKRPSKHLRPPRPLSSSHAAAQTKRDGRWMVQTMPGQAAVKDY
                     RCPGCEHLVTQGTPHVVVWPLETPIGAQRAVDERRHWHTSCWNRRP"
     gene            3904..4698
                     /locus_tag="PLF56_03995"
     CDS             3904..4698
                     /locus_tag="PLF56_03995"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_017935530.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="alpha/beta fold hydrolase"
                     /protein_id="HOQ52756.1"
                     /translation="MALFTQLVGQGRPDVVFLHGLFGRGKNWAGIARSLAEHEHAGVL
                     FDLPNHGRSHWTPTFDYVQVADEVADELRLHLGSAASVNLVGHSMGGKVAMLIALRHP
                     ALVRRLVVIDIAPDISYQVRTSVPLVKAMRGLDLNALQSRREAEVQLAREVHEPEVRQ
                     FLLQNLKLDRRPGLRNHWHWEVNLDLLGDHLDEVAGWPSVAGSYAGPVLWIAGGESPY
                     IRPEHKPRMLQLFPTTHLVTIPGAGHWVHADAPEPLTAELLRFLES"
     gene            complement(4720..5175)
                     /locus_tag="PLF56_04000"
     CDS             complement(4720..5175)
                     /locus_tag="PLF56_04000"
                     /EC_number="5.2.1.8"
                     /inference="COORDINATES: protein motif:HMM:NF012387.2"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_function: GO:0003755 - peptidyl-prolyl cis-trans
                     isomerase activity [Evidence IEA];
                     GO_function: GO:0016018 - cyclosporin A binding [Evidence
                     IEA];
                     GO_process: GO:0000413 - protein peptidyl-prolyl
                     isomerization [Evidence IEA];
                     GO_process: GO:0006457 - protein folding [Evidence IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="peptidylprolyl isomerase"
                     /protein_id="HOQ52757.1"
                     /translation="MTGGAVTITGDRAAAPCTLHSFESLVKQKFYDDTACHRLSDSGM
                     FMVQCGDPTGSGSGGPGYRFADELKGDETYTAGTVAMANSGPDTNGSQFFILYRDSAL
                     PAAYTVFGKVDAASLKVIETMAAQGHDNSYGDGTGRPRNAFRITTASIG"
     gene            5471..6010
                     /locus_tag="PLF56_04005"
                     /pseudo
     CDS             5471..6010
                     /locus_tag="PLF56_04005"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_002513471.1"
                     /note="incomplete; partial in the middle of a contig;
                     missing C-terminus; Derived by automated computational
                     analysis using gene prediction method: Protein Homology.
                     GO_function: GO:0008817 - corrinoid adenosyltransferase
                     activity [Evidence IEA];
                     GO_process: GO:0009236 - cobalamin biosynthetic process
                     [Evidence IEA]"
                     /pseudo
                     /codon_start=1
                     /transl_table=11
                     /product="cob(I)yrinic acid a,c-diamide
                     adenosyltransferase"
     gene            complement(6102..6563)
                     /locus_tag="PLF56_04010"
     CDS             complement(6102..6563)
                     /locus_tag="PLF56_04010"
                     /inference="COORDINATES: protein motif:HMM:NF022199.2"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="DUF2550 domain-containing protein"
                     /protein_id="HOQ52758.1"
                     /translation="MGSVVYAAEVVLIILGALMVASLSWLWLRRRWLSRTGGTFACSL
                     RRDPSAPSSRWVLGVARYRDHELQWFPAFSLSLWPTQRFDRTQVRAGEQRRPSAAEAE
                     LLYDERRILSLDGPPGGVELAMAVGSLTGLLSWLEAAPPGMRYRVSPISPD"
     gene            complement(6582..6998)
                     /locus_tag="PLF56_04015"
     CDS             complement(6582..6998)
                     /locus_tag="PLF56_04015"
                     /EC_number="7.1.2.2"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_002516827.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_component: GO:0045261 - proton-transporting ATP
                     synthase complex, catalytic core F(1) [Evidence IEA];
                     GO_function: GO:0046933 - proton-transporting ATP synthase
                     activity, rotational mechanism [Evidence IEA];
                     GO_process: GO:0015986 - proton motive force-driven ATP
                     synthesis [Evidence IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="F0F1 ATP synthase subunit epsilon"
                     /protein_id="HOQ52759.1"
                     /translation="MAEGDTLYVDVVAADRRVWEGDALSIVLRTVEGDIGILPHHEPF
                     LAVLVPCATEILTPDGKREVVAIDGGFITVADNRVSVFSQYGRLAHEISVNEAERELM
                     EADKRLNSGDNSEETRRHHDRATAQLAAAKRAQQVR"
     gene            complement(7003..>7494)
                     /locus_tag="PLF56_04020"
     CDS             complement(7003..>7494)
                     /locus_tag="PLF56_04020"
                     /EC_number="7.1.2.2"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_016667716.1"
                     /note="Produces ATP from ADP in the presence of a proton
                     gradient across the membrane. The beta chain is a
                     regulatory subunit; Derived by automated computational
                     analysis using gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="F0F1 ATP synthase subunit beta"
                     /protein_id="HOQ52760.1"
                     /translation="DDYTDPAPATTFAHLDATTELSREVASRGLYPAVDPLTSSSRIL
                     DPQFIGEEHYRTATRVKQILQRNKELQDIIAILGVDELSEEDKVLVARARRIQQFLSQ
                     NTYMATKFTNVEGSTVPLADTIEAFTMICDGEVDHIAEQAFFNVGGMDMVMANWDRMQ
                     KEG"
BASE COUNT         1181 a         2559 c         2522 g         1232 t
ORIGIN      
        1 tcgacctgtt cgacgagacc gcgagcgctg cgggcagctt tccccactcg atgctcggct
       61 atgagcgcca ggcggtcgat gcctacattc gcgacatcga acgccagctg agttcagcca
      121 agcgcaaggt gcggtcgctg caacggcagg tggcgaccaa agagaccgag accgaccttt
      181 cgaagctcgg cgaccacgcc cgcgacgtgc tgcgcgccgc cgaggccgag gccgccgagc
      241 ttcgcaatca ggcccgtctg gacgccgatc gcatcgttgc gcaggccgcc gccgaagccg
      301 aagaggtggc gcacaacact caggccgaaa cgtccaccca tcgcgtcgcc aacctcgacg
      361 acctcaaccg gctgcgcgaa gaactcgcca accagaccgg ggccgagttg aacgcggccc
      421 ggcaagaggc ccagctgttg cgagatgcgg ccgagaacca tcggcgcatg gtgctggccg
      481 atgctgagcg caacgcgacc gcgctgcgcg agcaggcagc gctcgaagcc gagcaggtgc
      541 gtcaggccgc ccagaaagag gcggccgacc tgcgggccgc gatcgctgct gaacatgccc
      601 aggctctgga cgcgctgcag cgacaccagt acgaggtgac ccaacacgtc ggtcagctct
      661 cggaacaggc ccgactgcag tccgatcagt tcggcgccga catggcgcac caggtcgaac
      721 tgatgaacaa tcggcgcaac gtggctgcgt ccgaggccga caagatcgtg gccgacgccg
      781 acgaccgggc ccgggcgatc atcgccgagg ctcgcgccgc cgccgccaag cgtgagcgcg
      841 aggcgggcct ggccgaggcc gacaagatct cgaagctcaa cgaagagatc gcgcggctca
      901 gggtgcgtca cggcgagttg cagaagattc tgcgcagcct gtcgcagatg gccacctcga
      961 gcttcgatga cgaaccggcc gccgcggcgc aggccccggc gtccgctgcg cccgaacctc
     1021 agggccagcc cgccgcagag gctgcccagc cgaacctcga cgacgacgcg acccgggtgc
     1081 agcctgcggc ctcgggtggt tccaaccggc gctgagttcc gtgactgccc agccgaacga
     1141 agtcgggggc gacgagaaca gcgtcgccga cggcacggtg ccgacggcgg ggccgtcggc
     1201 tgcgccctcc gctgcgcagc cgacacccag ccgtccgcgc ggctggttgc accgtattcg
     1261 ccgggccacc cagcccacgc cgcccgacct cgacccggac gcgatccatg ccggtccacg
     1321 caacgagatc agcatggcgc agcgcagccc gttcgccgtc ggcttcttca tcaccctggg
     1381 cgcgctcacg gcgttcgggt tggtgttcgg tctggtccgc atcgcgtcgg tgctgctgct
     1441 ggtgatgctg gccctgtacc tggcgctcgg catgaacccg ctggtggagc ggctgtcgtc
     1501 gcgggggctg cggcgcggac tcgctgtgtt gttggtcgcc ctcggcgcgc tggcggtgct
     1561 ggcattggcg ttctgggccg tgctgccggt ggcctacgaa cagatcaacc ggttgctgct
     1621 gaatgccccc gcgctgctgc aggagctgcg ggccaacccg cagatcgccc aactcgacgc
     1681 tcgctacgac gtgatcaacc gggtcagtgc ctttctgacc tcaggtgaac tgctcagcac
     1741 cctgttcggc ggcttgtggg gtgccagcca ggcggttgcg ggcctggtgt tcagcaccat
     1801 gatcacgctg acgctgacct tgtacattct ggcgtcgatg cccaccttca aggacgcgat
     1861 cttcgagctc gcgccggcct cgcggcgggc ccgggtgcgc tatctggcca atgagatgtt
     1921 cggccgaatc ggtgggtacg tgaccggact gttcatcatc atcacctgcg ccgccaccgt
     1981 tgccttcttg ttcctgagca ttgtcgggct cgggcagtac gcgttggcgt tggcggtcgt
     2041 ggtggccttg tgcgcggtga ttccgctgat cggatcgacg ctgtcgatga tcatcgtgtc
     2101 gatcgtcgcg ttcacgttct cggccacgca gggcctgatc acgctggcgt tctttctcac
     2161 ctaccagcag atcgacaact acttcatcca gccccgggtc tttcagcgct cggtcagcgt
     2221 gccggggccg ctggtcattc tggccgcggt ctgcggtggc ctgctgtacg gcctgctcgg
     2281 tgcgctgttg gcgatcccgt ccatggcggc gctgatgctg ctctaccgcg aggtactgat
     2341 tccggcactc gaccgccgct gacctgtcgc ttgatcggga cccggccacc gctggttgag
     2401 cttgtcgagt cctccggctc gaaccttctt ggttgcgttg ggtgccgcgg attgggggtg
     2461 caccccgtcc cgccccaggc agcctcaccg gctccgcaag ctccgccggt gagttgcccg
     2521 acgcccgacc acaccctgac ggggtgcacc cccactccgc ttcaatcgtg cgttgggtgg
     2581 cccggcgttc tcgctggtcg agcttgtcga gtcccgagcg gtggtcgttg gtgtgacacg
     2641 ttgggcagct acgttgttgt gaacgaagct gcgggacggg cgcccgggca cggttcacca
     2701 cctatctgtc gagtcctcac ggcagctgcg atctcgacat cgtcgcttga tggcgggata
     2761 cctcggcaat ttcgtgttgc aacccgaaac tggacggcgt tcccgccagc ggacagcgat
     2821 gtcgagatcg ctacagacct ctgggcggtc ggttgtgacc cgactcagaa caagcggtcc
     2881 tcggcgttgt ccagaccgcg aagggcgtcg tagtccacgc tgacgcaggt gatgccgcgg
     2941 gtgccggcca aggtcttggc ctgcggcttg atcagctggg cggcgaacac acccttcacc
     3001 ggggccagca gcgggtcggc gttcatcagg gccagatacc gggtcaactg ctccacgccg
     3061 tcgatctcgc cgcgccgctt cacctccacc gccacgtagc cgccggcgtc gtcgcgaaac
     3121 agcaggtcga ccgggccgat cggggtgagg tactcgcgct gcaccagggt ccagcccggg
     3181 ccgaacgctt cgggatgctc ggcgagcagc acctgcagat gagcctccac cccgtccttg
     3241 accaggccgg ggtcgacgcc gagttcgtgg tgcgagtcgt gcagtacctc ggcgatcgag
     3301 atcaccaggg tgtcggcgtc cttgttgcgc agttcccaca cctcgctgaa ccccgagtca
     3361 gcctcggccg tcgtcacggt cagtgtgcac ggggggttca tccagttcag cggcttgtag
     3421 gcgcggtcgt cggcgtggat cgacaccgat ccgtccgcct tggcgagaat caggcgggtg
     3481 gccagcggca ggtgcgcgct gagccgcccg gcgtagtcga cctggcagcg ggcaacgatc
     3541 aaacgcaccc ggtcaactta cccaatccgt gcccgcccgg ccgcggcgcc cacgggcgca
     3601 gctcaacatg cgctcgcagt aggctcgtcc catgcccaaa cgacccagca agcacctgcg
     3661 cccgccgcgc ccgctgtcgt cgtcccatgc ggccgcacag accaagcgcg acggacgctg
     3721 gatggtgcag accatgccgg ggcaggccgc ggtcaaggac taccgctgtc ccggctgtga
     3781 gcacctggtc acgcagggca ctccgcacgt ggtggtctgg ccgctcgaga ccccgatcgg
     3841 tgcgcagcgc gccgtggacg agcgacggca ctggcacacc agttgctgga accggaggcc
     3901 ctgatggcgt tgttcacgca actggtcggg cagggacgtc cggatgtggt gtttctgcac
     3961 ggactgttcg gacggggcaa gaactgggcc ggaatcgcca ggtcgctggc cgagcatgag
     4021 cacgccggcg tgctgttcga cctgcccaac cacggccgtt cccactggac gccgacattc
     4081 gactacgtgc aggtcgccga cgaggtcgcg gacgaactac gcctgcacct gggcagcgcg
     4141 gcctcggtga acctggtcgg ccactcgatg ggcggcaagg tagccatgct gatcgcgttg
     4201 cgccaccccg cactcgtccg gcggctggtg gtgatcgaca tcgcccccga catctcgtac
     4261 caggtgcgca catcggtgcc gctggtcaaa gccatgcggg ggctcgacct gaatgcgctg
     4321 cagagccgcc gcgaggccga ggtccagctg gcccgcgagg tgcacgagcc cgaggtgcgg
     4381 cagttcctgt tgcagaacct caagctcgat cgccggcccg gattgcgcaa tcactggcac
     4441 tgggaggtca acctcgacct gctcggcgac catctggacg aggtggcggg ctggccgtcc
     4501 gtcgccggca gctacgccgg gccggtgctg tggatcgccg gcggcgaatc gccctacatt
     4561 cgtcccgaac acaaaccccg catgctgcag ttgttcccga ccacccacct ggtcaccatt
     4621 cccggcgccg gtcactgggt gcacgcggac gcgcccgagc ccctgaccgc cgaactgctg
     4681 cggtttctgg agtcgtgaag ccccagcgcc gcagcgccct caaccgatgc tggccgtcgt
     4741 gatcctgaac gcgttgcgcg gacgacccgt gccgtcgccg tagctgttgt cgtgcccctg
     4801 cgccgccatg gtctcgatca ccttcagcga cgccgcgtcc accttgccga agaccgtgta
     4861 ggccgccgga agagcggaat cccggtagag gatgaagaac tgcgacccgt tggtgtcggg
     4921 cccggagttg gccatcgcca cggtgccggc ggtgtaggtc tcgtcgccct tgagttcgtc
     4981 ggcgaagcgg taaccgggtc ctccactgcc gctgccggtc gggtcgccgc actgcaccat
     5041 gaacatgccc gaatcggaca gccgatgaca ggccgtgtcg tcgtagaact tctgcttgac
     5101 cagtgactcg aacgagtgca atgtgcacgg tgctgccgcc cggtcaccgg tgatcgtcac
     5161 cgcgccgccg gtcatgcgca acgtgacgtg agggtgccgg tcttcggcac atccgtctcg
     5221 gagggcggct gcaccggccg ggcggccgag ccggccgccg ggtaggcgca ggtggcgccg
     5281 gcattcgccg gagcactgaa ctggccaccg gtcgggcggt cgtggcggtg gaacagccgc
     5341 tgaccagcgc agcgagcaga acgatcagag tgagcggacg gagcatttgc ccgagtctgc
     5401 cacagggcat cgcccgcctc accgcggcat gcgggcagcc ctgcgaatct gcgaccgata
     5461 ggctggcggc atggtccagc tcaccaagat ctacacgcgc accggcgatg cgggcaagac
     5521 tcgcctgtcg gacatgtcac tggccgccaa gacggacctt cgggtggagg cgtacgggca
     5581 ggtcgacgag gccaacgccg tgctgggggt ggcaactgcc cgcggcgacc tgcccgacca
     5641 gcttcagcag tccttgcggc tgatccagaa cgagttgttc gacctgggcg ccgatctgtc
     5701 cacacccctg gccgacaacc cgcagtggga gccgctgcgc atcgtccagc aatgcatcga
     5761 tcggctggag gggttctgcg acgagctttc cgcgcaggtc gggcccctga gctcgttcat
     5821 tctgccgggc ggaacgcaga ccgcagcaca gctgcacgtc gcgcgcacca tcgtgcggcg
     5881 cgccgagcgc accgcctggc gtgcggccga ggtgcacggg gtgggtgtcg aagggggtct
     5941 gaatccgctg gtgatcacgt acctcaaccg gctctcggac ctgctgttcc tggctgcccg
     6001 cgccgccaac gccgagcacg gggacgtgct atgggtgccg ggaaccgatc gcgaacccga
     6061 tgaccccagg gcagcgaggc gccgcgagcg tgccgaggcg gctaatcggg cgaaatcgga
     6121 gacacccggt atcgcatccc cggaggggca gcctccaacc agctgagcag gcccgtcaac
     6181 gagccgaccg ccatcgcgag ctcgacgccg cccggcggcc cgtccaggct caggatgcgc
     6241 cgttcgtcat acagcagttc ggcctccgcg gcagagggac ggcgctgctc accggcccga
     6301 acctgcgtgc ggtcgaagcg ctgagtcggc cacagcgaca gcgagaaggc cggaaaccac
     6361 tgcagttcgt ggtctcgata gcgagcgact ccgagcaccc accgggaact cggagccgaa
     6421 gggtcacgcc gcaggctaca cgcgaaggtg ccgccggtgc gcgagagcca gcgacggcgc
     6481 agccacaacc acgacagcga ggcgaccatg agcgcaccaa gaatgatcag caccacttcg
     6541 gcggcgtaga ccacgcttcc cacaggtgtc cgttcctcgg ctcagcggac ctgttgggcc
     6601 ctcttggcgg cagcgagctg agccgtggca cgatcgtgat gccggcgggt ctcttcgctg
     6661 ttgtcgccgg aattcagtcg cttgtcggct tccatcaact cgcgctcggc ctcgttgacc
     6721 gagatttcgt gcgccagtcg tccgtactgg ctgaacaccg agacccggtt gtcggccacc
     6781 gtgatgaagc caccgtcgat cgcgaccact tcacgtttgc cgtcgggggt caggatctcg
     6841 gtcgcgcatg gcaccagcac cgccaggaac ggttcgtggt gcggcaggat gccgatgtcg
     6901 ccctcgaccg tgcgcaacac gatcgacagt gcgtcgccct cccacaccct gcggtcggcg
     6961 gcgaccacgt cgacgtacag cgtgtcgccc tctgccatcg ggtcagccct ccttctgcat
     7021 gcggtcccag ttggccatca ccatgtccat gccgcccacg ttgaagaacg cctgctcggc
     7081 gatgtggtcg acctcgccgt cgcagatcat tgtgaacgcc tcgatggtgt cggcgagcgg
     7141 cacggtggaa ccctcgacgt tggtgaactt cgtggccatg taggtgttct ggctgaggaa
     7201 ctgctgaatg cggcgcgctc gcgcgaccag caccttgtct tcttcgctca actcgtcgac
     7261 gcccaggatc gcgatgatgt cttgcagctc cttgttgcgc tgcagaatct gcttcacccg
     7321 ggtggcggtg cggtagtgct cctcgccgat gaactgggga tcgagaatac ggctggaact
     7381 ggtcagcgga tcgaccgccg ggtagaggcc gcgggaggcg acctcacggg aaagctcggt
     7441 ggtggcgtcc aggtgcgcga acgtggtggc cggcgccggg tcggtgtagt cgtc
//
LOCUS       DAOSUG010000080         7471 bp    DNA     linear   ENV 10-JUN-2024
DEFINITION  MAG TPA_asm: Micropruina sp. isolate AMR_MDS_2195
            NODE_4922_length_7471_cov_4.156149, whole genome shotgun sequence.
ACCESSION   DAOSUG010000080 DAOSUG010000000
VERSION     DAOSUG010000080.1
DBLINK      BioProject: PRJNA925477
            BioSample: SAMN32864262
            Sequence Read Archive: SRR4236673
KEYWORDS    WGS; ENV; Metagenome Assembled Genome; MAG; Third Party Data; TPA;
            TPA:assembly.
SOURCE      Micropruina sp. (wastewater metagenome)
  ORGANISM  Micropruina sp.
            Bacteria; Bacillati; Actinomycetota; Actinomycetes;
            Propionibacteriales; Nocardioidaceae; Micropruina.
REFERENCE   1  (bases 1 to 7471)
  AUTHORS   Abdulkadir,N., Saraiva,J.P., Zhang,J., Stolte,S., Gillor,O.,
            Harms,H. and Rocha,U.
  TITLE     Genome-centric analyses of 165 metagenomes show that mobile genetic
            elements are crucial for the transmission of antimicrobial
            resistance genes to pathogens in activated sludge and wastewater
  JOURNAL   Microbiol Spectr 12 (3), e0291823 (2024)
   PUBMED   38289113
REFERENCE   2  (bases 1 to 7471)
  AUTHORS   Abdulkadir,N.
  TITLE     Direct Submission
  JOURNAL   Submitted (24-MAR-2023) Environmental Microbiology, Helmholtz
            Center for Environmental Research -UFZ, Permoserstr 15, Leipzig
            04318, Germany
COMMENT     The annotation was added by the NCBI Prokaryotic Genome Annotation
            Pipeline (PGAP). Information about PGAP can be found here:
            https://www.ncbi.nlm.nih.gov/genome/annotation_prok/
            
            ##Genome-Assembly-Data-START##
            Assembly Method        :: SPAdes v. 3.11.1
            Genome Representation  :: Full
            Expected Final Version :: Yes
            Genome Coverage        :: Not Applicable
            Sequencing Technology  :: Illumina MiSeq
            ##Genome-Assembly-Data-END##
            
            ##Genome-Annotation-Data-START##
            Annotation Provider               :: NCBI
            Annotation Date                   :: 05/04/2023 04:57:10
            Annotation Pipeline               :: NCBI Prokaryotic Genome
                                                 Annotation Pipeline (PGAP)
            Annotation Method                 :: Best-placed reference protein
                                                 set; GeneMarkS-2+
            Annotation Software revision      :: 6.5
            Features Annotated                :: Gene; CDS; rRNA; tRNA; ncRNA
            Genes (total)                     :: 2,776
            CDSs (total)                      :: 2,750
            Genes (coding)                    :: 2,714
            CDSs (with protein)               :: 2,714
            Genes (RNA)                       :: 26
            tRNAs                             :: 24
            ncRNAs                            :: 2
            Pseudo Genes (total)              :: 36
            CDSs (without protein)            :: 36
            Pseudo Genes (ambiguous residues) :: 0 of 36
            Pseudo Genes (frameshifted)       :: 2 of 36
            Pseudo Genes (incomplete)         :: 32 of 36
            Pseudo Genes (internal stop)      :: 2 of 36
            ##Genome-Annotation-Data-END##
FEATURES             Location/Qualifiers
     source          1..7471
                     /organism="Micropruina sp."
                     /mol_type="genomic DNA"
                     /submitter_seqid="NODE_4922_length_7471_cov_4.156149"
                     /isolate="AMR_MDS_2195"
                     /isolation_source="Activated sludge of municipal
                     wastewater treatment plants"
                     /db_xref="taxon:2737536"
                     /environmental_sample
                     /geo_loc_name="Canada"
                     /lat_lon="49.25 N 123.1 W"
                     /collection_date="2015-06-16"
                     /metagenome_source="wastewater metagenome"
                     /note="metagenomic"
     gene            174..308
                     /locus_tag="PLF56_04025"
     CDS             174..308
                     /locus_tag="PLF56_04025"
                     /inference="COORDINATES: ab initio
                     prediction:GeneMarkS-2+"
                     /note="Derived by automated computational analysis using
                     gene prediction method: GeneMarkS-2+."
                     /codon_start=1
                     /transl_table=11
                     /product="hypothetical protein"
                     /protein_id="HOQ52761.1"
                     /translation="MRRKRRGDLTDPGGGVCSAVERTNEATRASRARLAENVSKRDEL
                     "
     gene            complement(373..603)
                     /locus_tag="PLF56_04030"
     CDS             complement(373..603)
                     /locus_tag="PLF56_04030"
                     /inference="COORDINATES: ab initio
                     prediction:GeneMarkS-2+"
                     /note="Derived by automated computational analysis using
                     gene prediction method: GeneMarkS-2+."
                     /codon_start=1
                     /transl_table=11
                     /product="hypothetical protein"
                     /protein_id="HOQ52762.1"
                     /translation="MSGSAPEVRGDAGRTQRRVELVEYGNVILDASRLQNQCVRLPAA
                     REQREDLMDLINLGHRVRRPVNAGDAVDIVAV"
     gene            complement(728..4573)
                     /locus_tag="PLF56_04035"
     CDS             complement(728..4573)
                     /locus_tag="PLF56_04035"
                     /inference="COORDINATES: protein motif:HMM:NF014026.2"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_function: GO:0004553 - hydrolase activity, hydrolyzing
                     O-glycosyl compounds [Evidence IEA];
                     GO_process: GO:0005975 - carbohydrate metabolic process
                     [Evidence IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="glycoside hydrolase family 3 N-terminal
                     domain-containing protein"
                     /protein_id="HOQ52763.1"
                     /translation="MTAVAATTNSDQSALEVANAELSRSAANEGMVLLENHNHALPYA
                     KGGNVAVFGVGSYATVKGGTGSGQVNNRYNVSVRDGLTNAGYTITTSDAYYSAMKTAF
                     DTKYPPSTSGSISLSGGADYASVEQQLTRASVKPTAPTDRAVFVVARNSGEGADRKSS
                     AGDYLLTETELNDIQLIGQTYSNVTVLLNVGGVVDTSFFGQINKAANDPDGDQPIDGL
                     LLVSQGGQETGTVVADVLDGDVNPSGKLVDTWASSYSLYPASATFGANDGKPLLEEYS
                     EGIYVGYRYFDSFAKTLGADAVDYPFGYGLSYSDFQIATQSVKADGASVSVKARVTNV
                     GTKASGKEVVQVYFSAPAGSLDKPYQQLAGFAKTDVLKPGQAQTVTVRFNTADMASYS
                     QSKAAYVLEKGDYLIRVGNSSRNTAVAAKIALAADVTTEQLANEDNDNAPATELKADP
                     ANFYSYATQADEIAAAPTITLDPASIPSADHASALQQNVAVDSTSPYYAIDRDTISST
                     TAYVPEGQTDWAGTGAPYAAKTGETVQSVAVDPSATLYDVAKGKTSMEKFVAGLSVTQ
                     LGNLVEGASATGSTLSAAGAAGYTTAKYESLGIPAMTLADGPAGIRITQKIATSPVTY
                     QWCTAWPIGTMLAQTWNTDLVKQVGVAIGKEMKEYGVTLWLAPGMNIHRDPLNGRNFE
                     YYSEDPLITGLDAAAVTEGVQSNAGIGVTIKHYAANNQEVARNTTDAVISERAQREIY
                     LRGFEIAVKAAQPMAVMSSYNKINGTYTAGSYDLLTDLLRGEWGFKGLVMTDWGGVRA
                     GIVNTMYAGNDLIEPGGAPNDPINATIKNPPQIDISGLPVVNKLTTMSNNRTTYNWSF
                     NGLTPSATGATSVTTTVDSSTDLTKAPSSVSTVRDVINNETVTAHAKYASVNEAYTEV
                     KNFLANATGSTAGLTAAQAAGITLSDVKYATDGDNTTPVVAYTVTIKGNYPTAGQNMR
                     LGDLQRSAMRILNIAMQSAQFKQLAGVQGVSGIKVGSYTGQFDNLVNYLTQSKGKVGT
                     VSTTGPDFTVSTSPAASPSGWYKANVAVSVTLPDPDNDTAYVAVDSGVLGPYTGPVTV
                     SGEGTHLVKVLAVDEDGVPSQIKELPVNIDTTAPTAKADSVESGTLKLSASDALSGVA
                     GIEYSLDAGTTWKAYTAPVTVPKAPATLSYRATDKAGNVSAVKTVTVKGVLTLATPTI
                     SGTVAKSKTVTAKVSSYTKGAKLSYQWLRGGKAISGATKVSYKLVTADKGKKVSVTVT
                     ATLTDYTTVAKTSAAKTVK"
     gene            4902..5930
                     /gene="sbnA"
                     /locus_tag="PLF56_04040"
     CDS             4902..5930
                     /gene="sbnA"
                     /locus_tag="PLF56_04040"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_020384983.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_function: GO:0030170 - pyridoxal phosphate binding
                     [Evidence IEA];
                     GO_process: GO:0009058 - biosynthetic process [Evidence
                     IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="2,3-diaminopropionate biosynthesis protein SbnA"
                     /protein_id="HOQ52764.1"
                     /translation="MSQGGRRALVGAELDEAESLVGHTPLVPLRRLQDRTDLRVWAKL
                     EMFNPGGSAKDRTALAMLRRGWAEGSIGPDSIIVESSSGNLGVALARWCSRLELSFHC
                     VVDSKANPATVALIGALGGVVHRVTEPDAATGDLLAARMARVRELVATMRGAVWLNQY
                     ANPAALQAHAGTMREIAEALDHRVDALFVAVSTTGTLGGCRRYIQQHGMTTRVVAVDA
                     AGSVLFGGERSERHLTGFGAGTVPTLAQHLQPDGVARIGEVDTVIGCRLLAAREAIVA
                     GASSGAVVQAFLHAAPALPSGAQVVLLLHDGGVPYLDSVYDDGWVERRLGVGADELRR
                     RLSAAVPR"
     gene            5927..>7471
                     /locus_tag="PLF56_04045"
     CDS             5927..>7471
                     /locus_tag="PLF56_04045"
                     /inference="COORDINATES: protein motif:HMM:NF024846.2"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="FAD/NAD(P)-binding protein"
                     /protein_id="HOQ52765.1"
                     /translation="MMRVAIIGAGPKGLFAAERLGAHLQQRGAEARITVFDPQPPGWG
                     AGYRPDQPDWLRLNVNAAIVTADGRGSGRPSLGLPAFHTWRRERGERDPLDPFPPRAL
                     VGRYLADCWHELPGHLPAGVVLDHVARRVHQLTRSPHGWLIEGRCYDEVVLCTGHADD
                     WPGALVHRWRGPQPLVPRVYPVERWLSRDQVPAGCAVACRGAALTFIDAALSLTQGRG
                     GRFTQAGYQRSGDEPATIIPVGRRGAFLQVKPAPEGPLSALDLALPRARGLARCAASP
                     AGVGTVLRAVWRTAQDYLVAAGVQDATLPAPGEPLPADPDPVVALRRSLQVALGKRAP
                     GPAWALGQAWRDLYPAIVGVLGHDGCPEADWPRFAAAAALLEPLAFGPPPVNAAKLLA
                     LCEAGVIDARYLSGPWPDALRAADVAIDCVLPPPGLVEGQWPAQLCEAGVLVRGLGRR
                     GVAVAADASCLGSDGTPIRGLAALGRLTEDVVIGNDTLSRTLHPDADRWADRVALAAT
                     ARVGVTR"
BASE COUNT         1003 a         2478 c         2677 g         1313 t
ORIGIN      
        1 cacgcgaacg gtcaccgagc agtcctggtc ggcgctggag cgcttccgca accgacaccc
       61 agaccggaag accacggggg tcgtccttca cgggggtact caggtcgcac accttcacgg
      121 ctggctgcac gtgctgccga tgcggagcct ctgggatcac tgaacctcgc cgagtgcgtc
      181 gcaaacgccg aggcgacctg accgacccgg gtgggggagt ctgttcagcg gtcgagcgca
      241 ccaatgaggc gacgcgggcg tcccgggcgc ggttggccga gaatgtgagc aagcgcgatg
      301 agctgtgacg ccgaggtcag ttatcccttg gcacccctgt catcccggcg aaagccggga
      361 tctctctcct gatcagacag ccacgatgtc gaccgcgtca cccgcgttga ccgggcgcct
      421 taccctgtgg cccaggttga tcaggtccat cagatcctcg cgctgctcac gggcggccgg
      481 taggcgcaca cactggtttt gcaggcgact agcgtcgaga atgacgttgc cgtactcgac
      541 cagttcgacc cggcgctgag tccgtcccgc gtcgccgcgc acttccggcg ccgagccgct
      601 cacccgacga cgatccgttg gggacgcctc ccgggcgcga gccggccccg ctcacacgtc
      661 acccggccac tatgccactg tcgccgcccg gacgaaccgg acggcgacag tgacgctcag
      721 aaggaaacta cttgacggtc ttggcggcgg aggtcttcgc caccgtcgtg tagtccgtca
      781 gcgtggcggt gacggtcacc gacaccttct tgcccttgtc agcggtgacc agcttgtagc
      841 tgaccttggt tgcccccgag atggccttgc cgccccgcaa ccactggtag ctgagcttgg
      901 cgcccttggt gtagctggac accttggccg tgaccgtctt gctcttcgcg acggttccgc
      961 tgatcgtcgg ggtcgcgagc gtcaggacgc ccttgacggt gacggtcttg accgcggaca
     1021 cgttgcccgc cttgtcggtg gcccggtacg agagggtggc cggcgccttg ggcaccgtga
     1081 ccggtgccgt gtacgccttc cacgtggtgc ccgcatcgag ggagtactca atgccggcca
     1141 cacccgacag cgcgtccgac gcgctcagct tgagcgtgcc gctctccacc gaatccgcct
     1201 tcgcagtcgg ggctgtggtg tcgatgttga ccggcaactc cttgatctgc gacggcacgc
     1261 cgtcctcatc gacggcgagc accttcacca ggtgcgtccc ctcaccggag accgtcaccg
     1321 gtccggtgta ggggcccagc accccggagt cgacggcgac gtaggcggtg tcgttgtcgg
     1381 gatccggcag ggtgacgctg accgcgacgt tcgctttgta ccaaccggac ggggacgccg
     1441 ccgggctggt gctcacggtg aagtcgggtc cggtcgtgct gaccgtgccc accttgccct
     1501 tgctctgggt gaggtagttc accagattgt cgaactgccc ggtgtacgag ccgaccttga
     1561 tgcccgagac accctgcacg cccgccagtt gcttgaactg tgccgactgc atggcgatgt
     1621 tcagaatgcg catggcgctg cgttgcagat cgccgagccg catgttctgg ccggcggtgg
     1681 ggtagttgcc cttgatcgtc acggtgtagg ccaccaccgg ggtggtgttg tcgccgtccg
     1741 tggcgtactt cacgtcactg agcgtgatgc cggccgcctg cgccgcggtc aggccggcgg
     1801 tcgaacccgt ggcattggcc aggaagttct tcacctcggt gtaggcctcg ttgaccgagg
     1861 cgtacttggc gtgggcggtc accgtctcgt tgttgatcac gtcgcgcacc gtgcttaccg
     1921 aggacggggc cttggtcagg tcggtgctcg aatccaccgt ggtggtgacg ctcgtggcgc
     1981 cggtcgccga cggcgtgagc ccgttgaagc tccagttgta ggtggtgcgg ttgttcgaca
     2041 tcgtggtcag cttgttcacc accggcaggc ccgagatgtc gatctgcggc ggattcttga
     2101 tcgtcgcgtt gatcggatcg ttcggcgctc cgcccggctc gatcaggtcg ttgccggcgt
     2161 acatggtgtt gacgatgcct gcccgcacac cgccccagtc ggtcatcacc aggcccttga
     2221 agccccactc gccgcgcagc aggtcggtca gcaggtcgta cgagccggcg gtgtaggtgc
     2281 cgttgatctt gttgtaggaa ctcatcacgg ccatcggctg ggccgccttc accgcgatct
     2341 cgaagccgcg caggtagatc tcgcgctgcg cccgttcgct gatcaccgcg tcggtggtgt
     2401 tgcgggcgac ctcctggttg ttggcggcgt agtgcttgat cgtgaccccg ataccggcgt
     2461 tgctctgcac gccctcggtg accgcggcag cgtccaggcc ggtgatcagc ggatcctcgg
     2521 agtagtactc gaagttgcgg ccgttcagcg ggtcacggtg aatgttcatg cccggggcca
     2581 gccacagcgt gacgccgtac tccttcatct ccttgccgat cgcgacgccg acctgcttga
     2641 ccaggtcggt gttccaggtc tgcgccagca tggtgccgat cggccaggcg gtgcaccact
     2701 ggtaggtgac cggcgaggtg gcgatcttct gggtgatccg gatgccggcc gggccgtcgg
     2761 ccagcgtcat cgccggaatg cccagcgact cgtacttggc ggtggtgtag ccggcggccc
     2821 ctgccgccga cagggtcgaa ccggtggcgc tggcgccctc gacgaggttg ccgagctggg
     2881 tgaccgacaa tcccgcgacg aacttctcca tcgacgtctt gcccttggcc acgtcgtaca
     2941 gggtcgcgga cgggtccact gcgacgctct gcacggtctc gccggtcttc gcggcgtagg
     3001 gtgcgccggt gcctgcccag tccgtctggc cttcgggcac atacgccgtc gtggacgaga
     3061 tcgtgtccct gtcgatcgcg tagtagggcg aggtcgaatc gaccgcgaca ttctgctgca
     3121 gtgccgaggc atgatcggcg ctcggaatgc tcgccggatc aagggtgatc gtcggcgcgg
     3181 ccgcgatctc gtccgcctgc gtggcgtagc tgtagaagtt ggccggatcg gccttcagtt
     3241 ccgtggcggg agcgttgtcg ttgtcttcgt tggcgagttg ctcggtggtc acgtcggcgg
     3301 ccagcgcaat cttcgccgcg accgcggtgt tgcgcgagga attgccgacc cggatcaggt
     3361 agtcaccctt ctcgagaacg taggccgcct tgctctggct gtaggacgcc atgtcggcgg
     3421 tgttgaaccg aaccgtcacc gtctgcgcct ggccgggctt gagcacgtcc gtcttggcga
     3481 agccggcgag ctgctggtag ggcttgtcga ggcttccggc gggcgccgag aagtagacct
     3541 gcacgacctc cttgccgctg gccttcgtgc cgacgttggt cacccgggcc ttgacgctca
     3601 ccgaggcgcc gtccgccttg acgctctggg tggcgatctg aaagtccgag tacgacagcc
     3661 cgtagccgaa cgggtagtcc accgcgtcgg cccccaacgt cttggcgaac gagtcgaagt
     3721 agcggtaacc gacgtagatg ccctcggagt attcctccag cagcggcttg ccgtcgttgg
     3781 caccgaacgt ggccgaggcc gggtacagcg agtaactgga cgcccacgtg tcgaccagct
     3841 tgccgctggg gttgacgtcg ccgtcgagca cgtcggcgac cacggtgccc gtctcttgcc
     3901 cgccctggct gaccagcagc aggccgtcga tcggctggtc accgtcgggg tcgttggccg
     3961 ccttgttgat ctgcccgaag aaggacgtgt cgaccacgcc gcccacgttc aacaacacgg
     4021 tgacgttgga ataggtctgg ccgatcagct gaatgtcgtt gagctcggtc tcggtcagca
     4081 gatagtcgcc ggcgctcgac ttgcggtcgg cgccctcgcc cgagttgcgg gccaccacga
     4141 agaccgcccg atcggtgggc gcggtcggct tgacgctggc gcgggtgagt tgctgctcga
     4201 ccgaggcgta gtcggcgccg ccgctgaggc tgatgctgcc ggaggtcgac ggcgggtact
     4261 tggtgtcgaa ggccgtcttc atcgccgagt agtaggcatc gctggtggtg atcgtgtagc
     4321 cggcgttggt caggccgtcg cgcacgctga cgttgtagcg gttgttgacc tggcccgaac
     4381 ccgtcccgcc cttcacggtg gcgtacgagc ccacgccgaa cacggcgaca ttgccgccct
     4441 tggcgtaggg cagcgcgtgg ttgtggttct cgagcagcac catgccctca ttggcggcgc
     4501 ttctcgacaa ctcggcgttg gccacctcga gggcgctctg gtcggagttg gtggtcgcgg
     4561 cgacggcggt cacgccttgg ccgagggtga ggctggcagc cagaacgact gggactgcgg
     4621 cgctgaacag atgacgcgtt cggcgacgtg gagtcatggg ggattcctct ctggacgacg
     4681 gtgtcacagc gggtggggag gggaagcgct tcagttcgca ccctagaacc ggactcgttc
     4741 acaggtgacc caagcgcaaa agacgtgaga ggtggtgaat agtgaccggt tttcgcccca
     4801 gcacaggcgc cgatggtgag aaagggtgtc ccgggcggac cgacacgatt acccaccccc
     4861 gggtggggtt gtttcgcgga cgggctggct acggttgcag catgtcccag ggtggtcgtc
     4921 gcgcactcgt gggcgctgag ctcgacgagg cggagagcct ggtcgggcac acccccctgg
     4981 tgccgctgcg gcggcttcag gatcgcaccg acctcagggt gtgggccaag ttggagatgt
     5041 tcaacccggg cggcagcgcg aaggaccgca cggcgctggc gatgctgcgg cgcggctggg
     5101 ccgagggatc gatcgggccg gactccatca tcgtcgagtc gtcctcgggc aacctcggcg
     5161 tcgccctggc ccgttggtgc agccggttgg agttgtcgtt tcactgcgtg gtcgacagca
     5221 aggcgaaccc ggcgaccgtt gcgttgatcg gtgcgctcgg cggcgtcgtc catcgggtga
     5281 ccgagcccga tgcggccacc ggtgacctgc tggccgcccg catggcccgc gtccgtgagc
     5341 tggtggccac catgaggggc gcagtgtggc tcaaccagta cgccaatccc gcagcgctgc
     5401 aggcccacgc gggcaccatg cgcgagatcg ccgaggcgct cgaccaccgc gtcgatgccc
     5461 tcttcgtcgc ggtcagcacc accggcaccc tgggcggctg ccgccggtac atacagcagc
     5521 acggcatgac cacgcgggta gtggcggtcg acgctgcggg cagcgtgctg ttcggtggcg
     5581 agcggtccga gcggcacctg acgggtttcg gtgccggcac ggtgccgacg ctggcccagc
     5641 acctgcaacc cgacggcgtc gctcgaatcg gcgaggtcga caccgtgatc ggctgccgac
     5701 tgctggccgc gcgggaggcg atcgtcgcgg gcgcctcgtc cggggcggtg gtgcaggcat
     5761 tcctgcacgc cgcacccgcg ctgccctccg gcgcgcaggt ggtgctgctg ctccacgacg
     5821 gcggcgtgcc ctatctcgac tcggtctacg acgacggttg ggtcgagcgc cggctcggcg
     5881 tgggcgcgga cgagttgcgg cgccggctga gtgcggctgt gcctcgatga tgcgggtcgc
     5941 gatcatcgga gcgggcccga aaggactgtt cgccgccgag aggctggggg cgcatctgca
     6001 gcagcgcggg gccgaggcgc ggatcaccgt cttcgacccg cagccgcccg ggtggggcgc
     6061 cggctaccgg cccgaccagc cggactggct gcgcctgaac gtcaacgccg cgatcgtgac
     6121 agccgatggt cgcgggtccg gacgcccgtc gctcggcctg cccgctttcc acacctggcg
     6181 gcgggagcgg ggcgagcgtg acccgctcga cccctttccc ccgcgcgcac tggtcggccg
     6241 atacctggcc gactgctggc acgagttgcc ggggcacctg cccgcggggg tggtgctgga
     6301 ccacgtcgcg cgccgggtgc accaactgac gaggagtccg cacggctggc tgatcgaggg
     6361 tcggtgctac gacgaggtgg tgctctgcac cggtcacgcc gacgactggc cgggcgcgct
     6421 ggtgcaccgg tggcgcggcc ctcagccgct ggtgccccgg gtctacccgg ttgagcggtg
     6481 gctgagccgc gaccaggtac cggccgggtg cgcggtcgcc tgccgtggtg cggcgctgac
     6541 cttcatcgac gcagcgctca gcctgacgca gggccggggc ggacggttca cgcaggcggg
     6601 ctaccaacgc agtggcgacg agccggccac catcattccc gtcggacgcc ggggggcctt
     6661 cctgcaggtc aagcccgcac cggagggtcc gctgagtgca ctcgacctcg ccctgccccg
     6721 ggctcgtggg ctggcccgct gcgccgcctc gcctgccggg gtgggcacgg tgctgcgtgc
     6781 ggtctggcgc acggcgcagg actacctggt ggcggccggc gtccaagacg cgaccctgcc
     6841 cgcgcccgga gagccgctgc cggccgatcc cgacccggtg gtcgccctgc gtcgttcgct
     6901 gcaggtggcg ttgggcaagc gggcaccagg acccgcctgg gcgctgggcc aggcgtggcg
     6961 cgacctgtac ccggcgatcg tcggggtgct tgggcacgac gggtgtcccg aggcggactg
     7021 gccccggttc gccgccgcgg ccgcgctgct ggagccgctc gccttcgggc cgcctccggt
     7081 gaacgccgcc aagcttctcg cgctctgcga ggcgggggtg atcgatgccc gctatctatc
     7141 cgggccgtgg cccgacgccc tgcgcgcggc cgacgtggcg atcgactgtg tgctgccgcc
     7201 gcccgggctg gtggagggcc agtggccggc gcagctgtgc gaggcggggg tgctggtgcg
     7261 cggactcgga cgacgcggtg tcgcggttgc tgccgacgcg tcgtgcctgg gctccgacgg
     7321 caccccgatc cgcggcctgg ctgcgctggg acgcctgacc gaagacgtgg tgatcggcaa
     7381 cgacacgctg tcgcgcaccc tgcaccccga tgccgaccga tgggccgatc gggtggccct
     7441 cgccgccacg gcacgggtgg gggtgacccg g
//
LOCUS       DAOSUG010000081         7447 bp    DNA     linear   ENV 10-JUN-2024
DEFINITION  MAG TPA_asm: Micropruina sp. isolate AMR_MDS_2195
            NODE_4935_length_7447_cov_3.659226, whole genome shotgun sequence.
ACCESSION   DAOSUG010000081 DAOSUG010000000
VERSION     DAOSUG010000081.1
DBLINK      BioProject: PRJNA925477
            BioSample: SAMN32864262
            Sequence Read Archive: SRR4236673
KEYWORDS    WGS; ENV; Metagenome Assembled Genome; MAG; Third Party Data; TPA;
            TPA:assembly.
SOURCE      Micropruina sp. (wastewater metagenome)
  ORGANISM  Micropruina sp.
            Bacteria; Bacillati; Actinomycetota; Actinomycetes;
            Propionibacteriales; Nocardioidaceae; Micropruina.
REFERENCE   1  (bases 1 to 7447)
  AUTHORS   Abdulkadir,N., Saraiva,J.P., Zhang,J., Stolte,S., Gillor,O.,
            Harms,H. and Rocha,U.
  TITLE     Genome-centric analyses of 165 metagenomes show that mobile genetic
            elements are crucial for the transmission of antimicrobial
            resistance genes to pathogens in activated sludge and wastewater
  JOURNAL   Microbiol Spectr 12 (3), e0291823 (2024)
   PUBMED   38289113
REFERENCE   2  (bases 1 to 7447)
  AUTHORS   Abdulkadir,N.
  TITLE     Direct Submission
  JOURNAL   Submitted (24-MAR-2023) Environmental Microbiology, Helmholtz
            Center for Environmental Research -UFZ, Permoserstr 15, Leipzig
            04318, Germany
COMMENT     The annotation was added by the NCBI Prokaryotic Genome Annotation
            Pipeline (PGAP). Information about PGAP can be found here:
            https://www.ncbi.nlm.nih.gov/genome/annotation_prok/
            
            ##Genome-Assembly-Data-START##
            Assembly Method        :: SPAdes v. 3.11.1
            Genome Representation  :: Full
            Expected Final Version :: Yes
            Genome Coverage        :: Not Applicable
            Sequencing Technology  :: Illumina MiSeq
            ##Genome-Assembly-Data-END##
            
            ##Genome-Annotation-Data-START##
            Annotation Provider               :: NCBI
            Annotation Date                   :: 05/04/2023 04:57:10
            Annotation Pipeline               :: NCBI Prokaryotic Genome
                                                 Annotation Pipeline (PGAP)
            Annotation Method                 :: Best-placed reference protein
                                                 set; GeneMarkS-2+
            Annotation Software revision      :: 6.5
            Features Annotated                :: Gene; CDS; rRNA; tRNA; ncRNA
            Genes (total)                     :: 2,776
            CDSs (total)                      :: 2,750
            Genes (coding)                    :: 2,714
            CDSs (with protein)               :: 2,714
            Genes (RNA)                       :: 26
            tRNAs                             :: 24
            ncRNAs                            :: 2
            Pseudo Genes (total)              :: 36
            CDSs (without protein)            :: 36
            Pseudo Genes (ambiguous residues) :: 0 of 36
            Pseudo Genes (frameshifted)       :: 2 of 36
            Pseudo Genes (incomplete)         :: 32 of 36
            Pseudo Genes (internal stop)      :: 2 of 36
            ##Genome-Annotation-Data-END##
FEATURES             Location/Qualifiers
     source          1..7447
                     /organism="Micropruina sp."
                     /mol_type="genomic DNA"
                     /submitter_seqid="NODE_4935_length_7447_cov_3.659226"
                     /isolate="AMR_MDS_2195"
                     /isolation_source="Activated sludge of municipal
                     wastewater treatment plants"
                     /db_xref="taxon:2737536"
                     /environmental_sample
                     /geo_loc_name="Canada"
                     /lat_lon="49.25 N 123.1 W"
                     /collection_date="2015-06-16"
                     /metagenome_source="wastewater metagenome"
                     /note="metagenomic"
     gene            complement(<1..343)
                     /locus_tag="PLF56_04050"
     CDS             complement(<1..343)
                     /locus_tag="PLF56_04050"
                     /inference="COORDINATES: ab initio
                     prediction:GeneMarkS-2+"
                     /note="Derived by automated computational analysis using
                     gene prediction method: GeneMarkS-2+."
                     /codon_start=1
                     /transl_table=11
                     /product="hypothetical protein"
                     /protein_id="HOQ52766.1"
                     /translation="MTDQMAGETLAESTDTVVVQRSVSQPVKEVWRLLATPAGSEALL
                     GEGGVLGDKGDSWHSSNGTFGVVRSYHPLEQIRFSWHAEPDAPKSLVDLHLVQQSEDS
                     TVVEIRHEHIPG"
     gene            complement(1340..1915)
                     /locus_tag="PLF56_04055"
     CDS             complement(1340..1915)
                     /locus_tag="PLF56_04055"
                     /EC_number="3.2.2.-"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_011755921.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_function: GO:0003905 - alkylbase DNA N-glycosylase
                     activity [Evidence IEA];
                     GO_process: GO:0006284 - base-excision repair [Evidence
                     IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="DNA-3-methyladenine glycosylase"
                     /protein_id="HOQ52767.1"
                     /translation="MDLTDDVLSVAPALLGAHLCVNGVTLRLTEVEAYSGANDPAAHA
                     YRGWRPHTRHLFEPPGTLYCYRSYGIHICANIVCGPAGSGAAVLLRAGEVVDGLDLAR
                     RRRPGVPDARLARGPGCLGQVLGLSLADSGRTVGEPELSLQFADAAPGPIATGPRVGV
                     SVAARRPWRYWLVGEPSVSAYRASPRLPREL"
     gene            1966..2874
                     /locus_tag="PLF56_04060"
     CDS             1966..2874
                     /locus_tag="PLF56_04060"
                     /inference="COORDINATES: ab initio
                     prediction:GeneMarkS-2+"
                     /note="Derived by automated computational analysis using
                     gene prediction method: GeneMarkS-2+."
                     /codon_start=1
                     /transl_table=11
                     /product="hypothetical protein"
                     /protein_id="HOQ52768.1"
                     /translation="MRMSALAVAVAAFGLLTGCVQPATPSPTPSVTEVNLDLTAPGQA
                     RSMVRKLIAKAGTPQLIQVEITQQWAAITVVKNDQPETWAWRDNTIKKVDSDVAYVQQ
                     RIFSIDDFNISDVGGLFRAASGMAGSDGNQELQIVDSSAGEVFMSVSTNPESRTVFFY
                     PSGALLPILDFNTEGGIRDGLADVIGSRSSALAIGVQSNLGAWLDLTGSTTTTIRRLR
                     TATVPVTINERSQRSDLPTFSVSRLHPEVIWQVLDQARQRGDFDAGTRWQVTVDDRSR
                     LGVPRMYFTVGNDSFVTDLEGHEITP"
     gene            complement(2994..3845)
                     /locus_tag="PLF56_04065"
     CDS             complement(2994..3845)
                     /locus_tag="PLF56_04065"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_013865296.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="neutral zinc metallopeptidase"
                     /protein_id="HOQ52769.1"
                     /translation="MQYRDSANLDSSQVTSSSGGGAGGKMAVGGIGGLIIVVLAMVFG
                     FDPGSLVGGTASGPQQTTDQYAECKTGADIEGNRDCRFVAYTNSIQNYWSKAMQGYQK
                     TTTHIFTGQVSTGCGTATSAVGPFYCPNDRIVYLDTGFFDQLTGQLGAQGGDAAEAYV
                     IAHEYGHHISNLTGVLGKVQSAGNQTGANSPQVKLELQADCYAGVWLANASKDPNSPI
                     AAITKDDVFKAADAARAVGDDRIQQQATGRVDRESWTHGSSAQRQNWLARGFNSGDPN
                     QCDTFAG"
     gene            3961..4689
                     /locus_tag="PLF56_04070"
     CDS             3961..4689
                     /locus_tag="PLF56_04070"
                     /inference="COORDINATES: protein motif:HMM:NF012514.2"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_function: GO:0016787 - hydrolase activity [Evidence
                     IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="NUDIX domain-containing protein"
                     /protein_id="HOQ52770.1"
                     /translation="MQIVGVVVAGGSSVFVRPLEHGADPHRLAWELGYRIVRPLSATG
                     QGDDLTFTVQVSAHGRKVSERGLHRRRALSHDLTEAEISAPLVRQRLAAYAIVLSDRG
                     VLATEFSERTAVPGSWGLPGGGIDPGENPSQAVIREAIEETGQHIELAHLLDIQTDHW
                     IGRSPAGVIEDFHAVRIIYAATCPNPTDPVVNDVGGTTASTRWVPLHQWQRMHWAAGT
                     RALLDRHLMRLAMQLGYRRRWAAG"
     gene            complement(4753..5613)
                     /locus_tag="PLF56_04075"
     CDS             complement(4753..5613)
                     /locus_tag="PLF56_04075"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_013865306.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="Bax inhibitor-1/YccA family protein"
                     /protein_id="HOQ52771.1"
                     /translation="MRSSNPVLSRPDAFTPQQQPVQGGFQQQPYGYQQQPNQGGFQQP
                     GYGQPGQYPNPYGPQNPTQAARGVMTIDDVITKSAITLGTVIATAALTFMFMPARFLV
                     AGMIISALVGFVTVMLVAARRVINPTMVLAYAAIEGVFIGAVSKLYESLWNGIVPAAV
                     LATVVAAAATLGAYKFFRIKVTSKFRKMVMIGTMAYAGVLLVNFVMSLFGVNFLFGHG
                     NMALLLLISAVGVGLAVFNLIMDFDYIEQGIAMQAPESESWRAAFGLTVTLVWLYIEM
                     LRLLSYFRSN"
     gene            complement(5767..5840)
                     /locus_tag="PLF56_04080"
     tRNA            complement(5767..5840)
                     /locus_tag="PLF56_04080"
                     /product="tRNA-Leu"
                     /inference="COORDINATES: profile:tRNAscan-SE:2.0.12"
                     /note="Derived by automated computational analysis using
                     gene prediction method: tRNAscan-SE."
                     /anticodon=(pos:complement(5804..5806),aa:Leu,seq:taa)
     gene            complement(5856..6794)
                     /locus_tag="PLF56_04085"
     CDS             complement(5856..6794)
                     /locus_tag="PLF56_04085"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_008599108.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_function: GO:0000166 - nucleotide binding [Evidence
                     IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="Ppx/GppA phosphatase family protein"
                     /protein_id="HOQ52772.1"
                     /translation="MKVAAIDCGTNSVRLLVAEGTDGGGLVDLDRRLILTRLGQGVDA
                     TGRFHPDALARTLDAFAEYAAVLAGFGVDRVRLVATSAARDASNREDFFSGARARLGV
                     DPEIISGDEEAGLSFLGALTALPNVAAPALVMDIGGGSTELIVGSGADRAGAPRVDHG
                     LSLDIGSVRIRERYLASDPPSAEQVAAARAHVDALLDGAGIDFATVATWIGVGGTATS
                     LSALNQRLPAYDRAAVHGSSMTREDLHRLTAELLAATVEQVRALPSMHPKRADVICAG
                     ALIADRVGARMPVDLTISESDILDGIALRLLHGTNP"
     gene            complement(6791..7291)
                     /locus_tag="PLF56_04090"
     CDS             complement(6791..7291)
                     /locus_tag="PLF56_04090"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_020576128.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="DUF501 domain-containing protein"
                     /protein_id="HOQ52773.1"
                     /translation="MSTSSSIDPADLASIEEQLGRTPRGLLRVAWRCADGSPGVVVTA
                     PRLPDGTPFPTVYYLTCPLAVASTSTLEAQGVMAQMTARLADDADLAAHYRRAHLAYL
                     ADRRALGDVPEIDSVSAGGMPTRVKCLHVLVAHALAAGPGVNQLGDEAVELLKDSPFG
                     ACLGAQ"
BASE COUNT         1237 a         2504 c         2558 g         1148 t
ORIGIN      
        1 cgcccggaat gtgctcgtgg cggatctcga cgaccgtcga gtcctcggac tgctgtacca
       61 gatgcaggtc gaccaacgac ttgggggcgt cgggctcggc gtgccaactg aaccggatct
      121 gctccagcgg atggtagctg cgcaccacgc cgaaggtgcc gttggacgag tgccaactgt
      181 cacccttgtc gcccaggact ccaccctcgc ccagcagggc ctctgagccg gcgggagtgg
      241 cgagcaggcg ccacacctct ttgactggct gggacaccga tcgctggacg acgacggtgt
      301 cggtggactc tgcgagcgtt tcgcccgcca tctgatctgt catagaaacc tcgatccgaa
      361 tcggtccgaa cccggtcaac agcgaccggt ggcaccacca tacccgggcg ggcactggcg
      421 cgggcggcgt gtcgctacac ccgtctcagc gatcggcggg ttacaaaaga agccacgacc
      481 ggaccgcgta tgaggtggcc cggtcgagtc ttttccacgg cttcccagcc cccgggtgtc
      541 cgctggacct cgagcgaagc ggagacgtct ggtacaggtg tgacgcctgc gtcagtcggt
      601 acagatggca agccaccacg gcgcggtctt tccagattcg tagtcggatc ttcgcatctg
      661 ctattgcgcg ccgcagcgca caggagtaga actgctgcta cgccgaatcc gacgaaccca
      721 gcggtcgcaa gatcaatcgg ttcgccacag tgattcagca cacggacgga gatcgaggct
      781 ctttgcacgt ggagcaatcc acgagcgttt cgcaagacgg gcgaacgagt cagcgaaccg
      841 accgtagttc ggaggtcgtc ttaacccgaa cgaacagggg tctgaccaac tcatacttgg
      901 tcagaccccg ccttcgttgt acggccgcct cggccccgat ccacccccat ctccgcctgt
      961 tgcgctccgt tggaacgccc ggacgtcgcc ggccacgctc ccagttgcgc ggtgtgcccc
     1021 tgcgcggacc ggcgggcacg ggtcggtgac accagattcg gtggacggtg aacgaccggg
     1081 tgggttttgc caccgcagtc ggtgccgaaa cccacccgct gtcatcaggt cacgacattc
     1141 gggaagacgc tgaaccttcg gcactcgtca tcccgggcct gccccgggat ccccgcccac
     1201 gcacaaatcc cggcggccca cgcagagatc caggcgtccg ccgggatgac gaggttcagg
     1261 agcccaacgg ttcccaacac ggctggacga ccgtcccaca cacttgggag aaccgtcccc
     1321 cacacgacgc tcggcacgcc tacagctcac gtggcagccg cgggctggct cggtacgccg
     1381 acaccgacgg ttcgcccacc agccagtacc gccaggggcg tcgagccgcc accgacaccc
     1441 ccacacgggg gcccgtggcg atcgggcccg gcgctgcgtc cgcgaactgt aggctgagct
     1501 cgggctctcc caccgtgcgt ccggagtcgg ccaggctcag ccccagcacc tgacccaggc
     1561 agcccggccc acgggccagc cgcgcgtccg gcacgccggg gcgccggcgt cgtgccagat
     1621 ccaggccgtc caccacctcg ccggcgcgca gcagcaccgc tgccccactg ccggctggcc
     1681 cgcagacgat gttggcgcag atgtgaatgc cgtaggaccg gtagcagtac agggtgccgg
     1741 gtggctcgaa gaggtgccgg gtgtgcggtc gccagccgcg ataggcgtgc gccgcaggat
     1801 cgttcgcgcc cgagtacgcc tcgacctcgg tgagccgcag cgtcacaccg ttcacgcaca
     1861 ggtgcgcgcc cagcagcgcg ggagcgacgc tcagcacatc gtcggtgagg tccacacgtc
     1921 cagtgtgacc cgcccgggct ggggacaccg cgatagtctc gcagggtgcg catgtctgcc
     1981 ctggccgtgg cggttgccgc gttcggcctt ctcacggggt gcgtgcagcc agccacacca
     2041 agccccacgc ccagcgtcac cgaggtgaac ctcgacctga ccgctccggg ccaggcgcgc
     2101 tcgatggtgc gcaagctgat cgccaaggcg ggtactccgc agctgatcca ggtcgagatc
     2161 acgcagcaat gggcagccat cacggtggtc aagaacgatc agcccgaaac gtgggcctgg
     2221 cgcgacaaca cgatcaagaa ggtcgacagc gacgtcgcct acgtgcagca gcggatcttc
     2281 tccatcgacg acttcaacat cagcgacgtc ggtgggctgt ttcgcgcggc gtccggcatg
     2341 gcgggctcgg acggcaacca ggaactgcag atcgtcgact cgtccgccgg cgaagtgttc
     2401 atgagcgtgt cgaccaaccc cgagtcgcgc acggtcttct tctaccccag tggcgcgttg
     2461 ctgccgattc tggacttcaa caccgagggc ggcattcgcg acgggctagc cgatgtgatc
     2521 gggtcacgca gcagcgcgct tgcgatcggg gtgcagtcga acctgggggc ctggctcgac
     2581 ctgaccggca gcaccaccac cacgatccga cggctgcgca cggccactgt gccggtgacc
     2641 atcaacgagc gatcgcagcg ctctgacctg cccacgttca gcgtgtcgcg gcttcacccc
     2701 gaggtgatct ggcaggtgct ggatcaggcc cggcagcgcg gcgacttcga cgccggcacc
     2761 cgttggcagg tgacggtgga cgaccgcagc cggctcggtg tgccccgcat gtacttcacc
     2821 gtcggcaacg actcgttcgt caccgacctg gagggccacg agatcacgcc gtaggtggcc
     2881 gacgatgcgg ctggccgtgc tcgccgagac gcaccacccc tgccctgggc cagtcagttc
     2941 attgggtagg tcaagccgtg caggtcaggt cagcggcagt tcagccgcac gcttcagccg
     3001 gcgaaggtgt cgcactgatt ggggtcgccg ctgttgaagc ccctggccag ccagttctga
     3061 cgctgagcgg acgagccgtg ggtccaggac tcgcggtcga cccgtccggt ggcctgctgc
     3121 tgaatgcggt cgtcgccgac ggcgcgagcg gcgtccgcgg ctttgaacac gtcgtccttg
     3181 gtgatcgccg cgatcggcga gttggggtcc ttggacgcat tggccagcca cacccccgcg
     3241 tagcagtcgg cctgcaactc gagcttgacc tgaggcgagt tggcgccggt ctggttgcca
     3301 gccgattgca ccttgcccag cacaccggtg aggttgctga tgtgatggcc gtactcgtgt
     3361 gcgatcacgt aggcctcggc ggcgtcgccg ccctgcgcgc cgagctgccc ggtcaactgg
     3421 tcgaagaagc ccgtgtcgag gtacacgatg cggtcgttgg ggcagtagaa cggaccgacg
     3481 gccgacgtgg cggtgccgca cccggtgctg acctgaccgg tgaagatgtg ggtggtggtc
     3541 ttctgatagc cctgcatcgc cttgctccag tagttctgga tcgagttggt gtaggcgacg
     3601 aagcgacagt cgcggttgcc ttcgatgtcg gcaccggtct tgcactcggc gtactggtcg
     3661 gtggtctgct gcgggccgga cgcggtgccg ccgacgagcg agcccgggtc gaagccgaaa
     3721 accatggcga gcaccacaat gatcaggccg ccgatgccac ccacggccat cttgccgccg
     3781 gccccgccgc ccgaactact ggtgacctgt gagctgtcga ggttggccga atcccggtac
     3841 tgcaccgcat cctccgtcgc ctgtgagttg tgggccgagt gtagtgggcg cgagcgcggc
     3901 atgtcggagg cgtcggggag tcggtttgga acgcctccgg gcctcagtca tcatgggcag
     3961 atgcagatcg tcggtgtggt ggtggcggga gggtcgagcg tcttcgtccg tccgctcgaa
     4021 cacggcgccg acccgcaccg gctggcctgg gagttggggt accgaatcgt ccggccgctg
     4081 tcggcgacgg ggcagggcga cgacctgacg ttcacggtgc aggtgtccgc ccatggacga
     4141 aaagtgtccg agcgtggcct gcaccggcgg cgggcgctca gccacgacct gaccgaggcc
     4201 gagatcagtg ccccgctggt gcggcagcgg ctggccgcgt acgccatcgt gctcagcgac
     4261 cgcggcgtac tggccaccga gttctccgag cgcaccgcgg tgcccggctc gtgggggctg
     4321 cccggtggcg gcatcgaccc gggcgagaac ccgagccagg cggtgattcg cgaggcgatc
     4381 gaagagaccg ggcagcacat cgagctcgcg cacctgctcg acattcagac cgaccattgg
     4441 atcggacgct cccccgccgg cgtcatcgag gactttcacg cagtacgcat catctacgcc
     4501 gcgacctgcc cgaaccccac cgatccggtg gtcaacgacg tgggcggcac cacggcgtcc
     4561 acccgttggg tgccgctgca ccagtggcag cgcatgcact gggccgccgg cacgcgcgcg
     4621 ctgctcgacc gacacctgat gcggctggcc atgcaactgg ggtatcgacg gcgctgggcg
     4681 gccggctgac gagtgcgcaa gaaacgaaac gccgccgacc cgagggccgg cggcgtttcg
     4741 tcgcgtatgg gatcagttgg agcggaagta cgacagcaac cgcagcatct cgatgtagag
     4801 ccagaccagc gtgacggtca ggccgaaggc cgcgcgccac gattcggact cgggcgcctg
     4861 catcgcgatg ccctgctcga tgtagtcgaa gtccatgatc aggttgaaca cggccagccc
     4921 gacgccgacc gccgagatca gcagcagcag cgccatgttg ccgtggccga acaagaagtt
     4981 gacgccgaac agcgacatca cgaagttgac cagcagcacg ccggcgtagg ccatcgtgcc
     5041 gatcatcacc atcttgcgga acttgctggt gaccttgatg cggaagaact tgtacgcacc
     5101 gagggtggcg gcggcggcga ccacggtggc gagcaccgcg gccggaacga tgccgttcca
     5161 caaggactcg tacagcttgc tgaccgcacc gatgaacacg ccctcgatgg cggcgtacgc
     5221 cagcaccatc gtggggttga tcacccgacg ggcggccacc agcatgaccg tgacgaagcc
     5281 gaccagtgca ctgatgatca tgccggccac gaggaagcgc gcgggcatga acatgaacgt
     5341 caaagcggcg gtggcgatga cggtgccgag cgtgatcgcc gacttggtga tcacgtcgtc
     5401 gatggtcatc acaccgcggg cggcctgcgt cggattctgc gggccgtagg gattcgggta
     5461 ctgaccgggc tggccgtagc cgggttgctg gaagccgccc tggttgggct gctgctggta
     5521 gccgtagggt tgctgctgaa agccgccctg gacgggctgc tgctgcggcg tgaaagcgtc
     5581 cggcctcgac aagacggggt tggagctgcg catggtgttg tttcctcctt gtggatgccc
     5641 attcatacta ggcaacgcac gaaagggtgg gaaccttcca caccactact cagggcaggt
     5701 tccgggtgat tggtgcacgc cgggcactgc cggtgcgctc ggacggtggc cgaaccgacc
     5761 tgggcgtgcc cccggcgggg ctcgaacccg cactggggcg ggtttaagcc gcctgcctct
     5821 gccggttggg ctacgggggc acgtcggcgt tgagtctacg ggttggtgcc gtgcagcagg
     5881 cgcagcgcga tgccgtccag aatgtcggac tcgctgatcg tcaggtccac cggcatccga
     5941 gcacccaccc ggtctgcgat cagggcgccg gcgcagatca cgtccgcacg cttgggatgc
     6001 atggacggca gtgcgcgcac ctgctcgacc gtggcggcca gcaactcagc ggtgagtcgg
     6061 tgcaggtcct cgcgggtcat ggacgagccg tgcaccgcgg cccggtcata ggccggcagg
     6121 cgttggttga gcgccgacag cgaggtggcc gtgcccccga cgccgatcca ggtcgcaacg
     6181 gtggcgaagt cgatgccggc gccgtccagc agggcgtcga cgtgggcgcg cgcggcggca
     6241 acctgctcgg cactgggcgg atcggaggcc aggtagcgtt ccctgatccg gacgctgccg
     6301 atgtcgagcg aaagcccgtg gtcgacgcgc ggcgccccgg cccggtcggc cccggagccg
     6361 acgatgagtt cggtggagcc tccgccgatg tccatcacca gggcgggggc agcgacgttc
     6421 ggcaatgcgg tcagcgcacc caggaacgac aacccggcct cttcgtcacc gctgatgatc
     6481 tcgggatcga cgccgagtcg cgcccgggcg cccgagaaga aatcctcacg gttggacgca
     6541 tcccgcgccg ccgacgtggc gaccaggcgc acccggtcga ccccgaagcc ggccagcaca
     6601 gccgcatact cggcgaaggc gtcgagggtg cgggccagcg cgtcggggtg aaagcgcccg
     6661 gtggcgtcca caccctgacc tagccgggtc aggatcagcc gccggtcgag gtcgaccagc
     6721 cccccaccgt ccgtgccctc ggcgaccagc agccgcaccg aattggttcc acagtcgatc
     6781 gccgcgacct tcattgcgcc cccagacacg caccgaacgg agaatccttc aacaactcaa
     6841 cggcctcgtc gcccagctgg ttgacccccg ggccggctgc cagcgcatgg gcgaccagca
     6901 cgtgcaggca cttgacccgg gtcggcatgc cgcccgcact gacggagtcg atctcgggca
     6961 cgtcgcccag ggcgcgccgg tcggccaggt acgccagatg ggcccggcgg tagtgcgcgg
     7021 caaggtcggc atcgtcagcc agccgtgccg tcatctgcgc catcacccct tgtgcctcga
     7081 gggtggacgt gctggcgacg gccagcggac aggtcaggta gtacacggtc gggaaggggg
     7141 tgccgtccgg tagccggggt gcggtgacca cgaccccggg gctgccgtcc gcgcaacgcc
     7201 acgccacccg gagcagcccg cgcggggtac gtccaagttg ttcttcgatc gaggcgaggt
     7261 cggccggatc gatgctggac gaggtggaca ccgcctcatt ctgccccgga ggtgcgggtg
     7321 tgtcgaagcg gaccggagcg ccgccgagat cggacgaacc ggatctggag aagcctgacc
     7381 cagctgatgg tcgagcttga aaccctgacc agtggaggtc tcgacgctcg gccaacggat
     7441 cggacgt
//
LOCUS       DAOSUG010000082         7428 bp    DNA     linear   ENV 10-JUN-2024
DEFINITION  MAG TPA_asm: Micropruina sp. isolate AMR_MDS_2195
            NODE_4952_length_7428_cov_3.563678, whole genome shotgun sequence.
ACCESSION   DAOSUG010000082 DAOSUG010000000
VERSION     DAOSUG010000082.1
DBLINK      BioProject: PRJNA925477
            BioSample: SAMN32864262
            Sequence Read Archive: SRR4236673
KEYWORDS    WGS; ENV; Metagenome Assembled Genome; MAG; Third Party Data; TPA;
            TPA:assembly.
SOURCE      Micropruina sp. (wastewater metagenome)
  ORGANISM  Micropruina sp.
            Bacteria; Bacillati; Actinomycetota; Actinomycetes;
            Propionibacteriales; Nocardioidaceae; Micropruina.
REFERENCE   1  (bases 1 to 7428)
  AUTHORS   Abdulkadir,N., Saraiva,J.P., Zhang,J., Stolte,S., Gillor,O.,
            Harms,H. and Rocha,U.
  TITLE     Genome-centric analyses of 165 metagenomes show that mobile genetic
            elements are crucial for the transmission of antimicrobial
            resistance genes to pathogens in activated sludge and wastewater
  JOURNAL   Microbiol Spectr 12 (3), e0291823 (2024)
   PUBMED   38289113
REFERENCE   2  (bases 1 to 7428)
  AUTHORS   Abdulkadir,N.
  TITLE     Direct Submission
  JOURNAL   Submitted (24-MAR-2023) Environmental Microbiology, Helmholtz
            Center for Environmental Research -UFZ, Permoserstr 15, Leipzig
            04318, Germany
COMMENT     The annotation was added by the NCBI Prokaryotic Genome Annotation
            Pipeline (PGAP). Information about PGAP can be found here:
            https://www.ncbi.nlm.nih.gov/genome/annotation_prok/
            
            ##Genome-Assembly-Data-START##
            Assembly Method        :: SPAdes v. 3.11.1
            Genome Representation  :: Full
            Expected Final Version :: Yes
            Genome Coverage        :: Not Applicable
            Sequencing Technology  :: Illumina MiSeq
            ##Genome-Assembly-Data-END##
            
            ##Genome-Annotation-Data-START##
            Annotation Provider               :: NCBI
            Annotation Date                   :: 05/04/2023 04:57:10
            Annotation Pipeline               :: NCBI Prokaryotic Genome
                                                 Annotation Pipeline (PGAP)
            Annotation Method                 :: Best-placed reference protein
                                                 set; GeneMarkS-2+
            Annotation Software revision      :: 6.5
            Features Annotated                :: Gene; CDS; rRNA; tRNA; ncRNA
            Genes (total)                     :: 2,776
            CDSs (total)                      :: 2,750
            Genes (coding)                    :: 2,714
            CDSs (with protein)               :: 2,714
            Genes (RNA)                       :: 26
            tRNAs                             :: 24
            ncRNAs                            :: 2
            Pseudo Genes (total)              :: 36
            CDSs (without protein)            :: 36
            Pseudo Genes (ambiguous residues) :: 0 of 36
            Pseudo Genes (frameshifted)       :: 2 of 36
            Pseudo Genes (incomplete)         :: 32 of 36
            Pseudo Genes (internal stop)      :: 2 of 36
            ##Genome-Annotation-Data-END##
FEATURES             Location/Qualifiers
     source          1..7428
                     /organism="Micropruina sp."
                     /mol_type="genomic DNA"
                     /submitter_seqid="NODE_4952_length_7428_cov_3.563678"
                     /isolate="AMR_MDS_2195"
                     /isolation_source="Activated sludge of municipal
                     wastewater treatment plants"
                     /db_xref="taxon:2737536"
                     /environmental_sample
                     /geo_loc_name="Canada"
                     /lat_lon="49.25 N 123.1 W"
                     /collection_date="2015-06-16"
                     /metagenome_source="wastewater metagenome"
                     /note="metagenomic"
     gene            complement(<1..1519)
                     /locus_tag="PLF56_04095"
     CDS             complement(<1..1519)
                     /locus_tag="PLF56_04095"
                     /inference="COORDINATES: protein motif:HMM:NF012382.2"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_function: GO:0030170 - pyridoxal phosphate binding
                     [Evidence IEA];
                     GO_process: GO:0009058 - biosynthetic process [Evidence
                     IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="aminotransferase class I/II-fold pyridoxal
                     phosphate-dependent enzyme"
                     /protein_id="HOQ52774.1"
                     /translation="MTAHEPPTEWLLNTDPALRWQVERDLLDADEQVWRATRGRIATE
                     GMGARLLAQQDPDGQWAGGAYFPADGHWSAWLQHQPGQPWTATTWSLFTLCRWGLDAD
                     VLAGTADKLAANSRWEYDDLPYWGGEVDVCINSFTLTSGTWLGADVAGLVGWFDDHQL
                     ADGGWNCEWQTGSTRSSYHSTLNALEALLFREVYGPAGESLAPLRRGGEEYLLSRGLR
                     LRASTGEQVGPWATLLAYPPRYRYDLLRALDYFRRAAAVDGAPPDARLAEAIARLSPP
                     LGRYPDVRPAREAIAARHGVDVSMVLPTSGGAEASTLIAHGLPLTNPMVVHPQFTEPE
                     AALRAAGRAVQRWLLPVTAGAGTPDLGELPSWADALFVGNPTNPTGWLHRREALLAAG
                     QGRLLVVDEAFMDATDEAESLIEPHMAGRLVLRSLSKTWGLAGLRVGYVVGDPALIAA
                     LERMQPPWSVSSPAIAAMIATASAEAVAEVAAVATTVRERRVRFVADLRVAGFGVP"
     gene            complement(1585..2721)
                     /locus_tag="PLF56_04100"
     CDS             complement(1585..2721)
                     /locus_tag="PLF56_04100"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_007078229.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="aminotransferase class I/II-fold pyridoxal
                     phosphate-dependent enzyme"
                     /protein_id="HOQ52775.1"
                     /translation="MSEQSWSPETAAVVAGRPPHRAGAAVSPPVELTSTYVSWEPFGA
                     TDPGIYGRMNNATWLALEEAVGALERGTALCFAAGMAAGAALFSLVPEGGRVVMADSC
                     YGSMLSLAQQLADAGRFALVLVDVSDTDAVVAAMPGADWLVLESPTNPLMQVADLIAL
                     GAAAARLGVRIAVDNTFATPLLQNPLELGASVVMHSGTKYLAGHSDVVLGLLVVRDEA
                     LLAQLRAYRGLHGGILGPMEAWLALRGLRTLAVRLERQQASAAVLADRLSEHPAISRV
                     RYPGLPSDPGHQRAKTQMRGFGGMLSIELADGRAAADDLVRGVRLWVPATSLGGVESL
                     IERRRRIPAEPHTVPENLVRLSVGLEHVDDLWADLEQALDRSAG"
     gene            complement(2877..4616)
                     /locus_tag="PLF56_04105"
     CDS             complement(2877..4616)
                     /locus_tag="PLF56_04105"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_016668478.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_function: GO:0016874 - ligase activity [Evidence IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="long-chain-fatty-acid--CoA ligase"
                     /protein_id="HOQ52776.1"
                     /translation="MSETLPAPWTRHYQPGVPAEIELPTESLVSLYERSVAEGGSAVA
                     TEFFGRETTYAELGEQIARAAEGLRALGVSAGDRVALILPNCPQHIVAFYAVLRLGAV
                     VVEHNPLYTARELHHQFDDHGARVVIAWDVAVAKLRDGDFELEPDHIVSVNLLKAFPT
                     LKRLALQLPVPRLRGLRDKLTQPAPGTVSWQKLLASPVLDPGHPRPSVHDLAVIQYTS
                     GTTSLPKGAMLSHYNLFANARQGEAWMHGAQYRKEVFYAILPMFHAFGMTLFMTYGIL
                     KQGRLVMFPSFDVTMVLDAAKKSPPTVYCAVPPIYEATAKSAKKRGISLRSAKYCISG
                     AMALPTATVQLWESVSGGLLVEGYGMTEASPVCLGNPFAPSRRTGTIGIPFPSTWMKV
                     VDPADPTVEVPQGQPGELLIKGPQVFSGYWNNPAETEKTLLPGGWLRTGDLVTVDADG
                     FTTIVDRVKELIITGGFNVSPSEVEEVLSEHPGVASAAVVGLPNEEVGGERVVAAVVP
                     QPDAQLTDADLRSWCKQRLTGYKVPRAFYVVDELPASMLGKVLRKKVRESLPDREPLP
                     RAPRPGKHRPKKG"
     gene            complement(4723..5358)
                     /locus_tag="PLF56_04110"
     CDS             complement(4723..5358)
                     /locus_tag="PLF56_04110"
                     /inference="COORDINATES: protein motif:HMM:NF019614.2"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="DUF1697 domain-containing protein"
                     /protein_id="HOQ52777.1"
                     /translation="MPHRHPLVELVRITHALVEPVETPAHTVSGIVQDDAMTNESRWV
                     GLLRAVNVGGRTLTNAQLKAAAADAGFTAVATLLASGNLVFTATGDEPIVRSSLEQVI
                     AADAGFTVEVLLRSKPQLQRLIEANPFPDGAPSHVLVCFLSDAAPSALAERLAEVAVN
                     ERIQVGEREVWIDFVDGIGRSRLAAKLPALVKPLVVTGRNFNTVVKLEALL"
     gene            5390..6346
                     /locus_tag="PLF56_04115"
     CDS             5390..6346
                     /locus_tag="PLF56_04115"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_017934084.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="3-methyladenine DNA glycosylase"
                     /protein_id="HOQ52778.1"
                     /translation="MSVTPHSPVPSATLLSAPVLSTPVLSTPVLSAPVLPEAEWRPRA
                     LAHDRRVRAWTEPYLQRRSRREPHPVEDFLWTYYSYRPGALRTWHPGWGATLSGDVAA
                     LVAVRGLLVEHDRAAVDPELPAARAGQVEQIRSLLVATQSRPARLGCFGLHEWAMVYR
                     QSAEQVRHAQVPLRLGAEGTDAVVEGHRITCTHYDAYRFFTEAAVPLNTRRPTLATRV
                     LNEQPGCLHAGMDLYKWAYKLAPITGAELIADCFELAREIRVLDMRASPYDLREWGYA
                     PVRIETPDGKADYVRTQRDFSTRSGVLRRRLIELCDAVLAAA"
     gene            complement(6360..6938)
                     /locus_tag="PLF56_04120"
     CDS             complement(6360..6938)
                     /locus_tag="PLF56_04120"
                     /inference="COORDINATES: protein motif:HMM:TIGR02246.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="SgcJ/EcaC family oxidoreductase"
                     /protein_id="HOQ52779.1"
                     /translation="MAKLKKLLKRAVARTPLPFPARSPAVNPLTTPLDLIHEYVRAWD
                     DKDADAIGRLFVEDADFVNVVGLWWTGRRSIVRAQRFGFQHAFKNASIKLLKVSQRFL
                     GDDAAVVMAQWQITGQLDPDNEPVDPRRGLISATLVKLGDGTWLGVSCQNTDIAMAAD
                     TNVSREGTLVATSYIKGPSAAEVAAAEWVESD"
     gene            complement(7112..>7428)
                     /locus_tag="PLF56_04125"
     CDS             complement(7112..>7428)
                     /locus_tag="PLF56_04125"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_020388816.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_function: GO:0016491 - oxidoreductase activity
                     [Evidence IEA]"
                     /codon_start=3
                     /transl_table=11
                     /product="FAD-dependent oxidoreductase"
                     /protein_id="HOQ52780.1"
                     /translation="VPGTEREIPADLVLLAMGFVGPEKKGLVEQLGVDLDPRGNVARD
                     ENYATSVPGVFVCGDAGRGQSLIVWAIAEGRSCANGVDQYLQGRSRLPKPILPTERQL
                     MV"
BASE COUNT         1134 a         2631 c         2548 g         1115 t
ORIGIN      
        1 cgggcactcc gaagccggcg acgcgaaggt cggccacgaa ccgaaccctg cgttcgcgca
       61 ctgtcgttgc cacggcggcg acctcagcga ccgcttcggc agaggcagtc gcgatcatgg
      121 cggcgatcgc cggcgacgac accgaccagg gcggctgcat ccgctccagg gcggcgatca
      181 gtgccgggtc gcccaccacg tagccgaccc gcagtccggc cagtccccag gtcttgctca
      241 gcgaccgcag caccagccgg cccgccatgt gcggctcgat cagggattcg gcctcgtcgg
      301 tggcgtccat gaacgcctcg tcgaccacca gcagccggcc ctgcccggcc gccagcaggg
      361 cctcgcgccg gtgcagccag ccggtcggat tggtcgggtt gccgacgaac aacgcgtccg
      421 cccaggacgg caactcgccc aggtcgggcg tcccggcccc tgccgtgacc ggcagcagcc
      481 agcgctgcac cgcccgcccg gccgcgcgta acgcggcctc gggttcggtg aactgggggt
      541 gcaccaccat cggattggtc agcggcaacc cgtgggcgat cagcgtggac gcctccgcac
      601 cgccgctggt cggcaacacc atcgagacgt cgacgccgtg ccgcgccgcg atcgcctccc
      661 gagccgggcg gacgtcggga tagcggccca gcggcgggga cagccgggcg atcgcttcgg
      721 ccagccgcgc gtccggcggg gcgccgtcga cggccgccgc ccggcggaag tagtccaggg
      781 cacgcagcag gtcgtagcgg tacctcggcg ggtaggccag aagggtcgcc cacgggccga
      841 cctgctcgcc ggtcgatgcc cgcagccgca gcccccgcga cagcaggtac tcctcgccac
      901 cgcgtcgcaa tggggccagt gactcaccgg caggtccgta gacctcacgg aacagcaacg
      961 cctccagcgc gttcagcgtc gaatgatagg acgagcgggt cgagccggtc tgccactcgc
     1021 agttccagcc gccgtcggcc agttgatgat cgtcgaacca gcccaccagg ccggccacgt
     1081 cggcgccgag ccaggtgccg ctggtgaggg tgaagctgtt gatgcagacg tcgacctcgc
     1141 cgccccagta gggcaggtcg tcgtactccc agcgactgtt cgcggcaagc ttgtcggcgg
     1201 tgccggccag gacgtcggcg tccagccccc agcggcacag cgtgaacagt gaccatgtgg
     1261 tggctgtcca gggctggccg ggctgatgct gcagccaggc cgaccagtgc ccgtcggccg
     1321 ggaagtacgc tcccccggcc cactggccat cggggtcctg ctgtgcgagc agacgggccc
     1381 ccattccctc ggtggcgatg cggccccggg ttgctcgcca cacctgctca tcggcgtcga
     1441 gcaggtcacg ttcgacctgc caacgcagcg ccggatcggt gttcagcagc cactcggtcg
     1501 gcggctcgtg ggcggtcatg gggtcacagt aaccaccgac ccggacgaaa tctgtccggt
     1561 tcgctcagcc cagatcctcc gggctcaccc tgcggagcgg tcgagcgcct gctcgaggtc
     1621 ggcccacagg tcgtccacgt gctcgagccc gaccgacagc cggaccaggt tctcaggcac
     1681 cgtgtgcggc tcggcgggaa tgcgccggcg gcgctcgatc agcgactcca cccctcccag
     1741 gctggtggcc ggaacccaga gccggacgcc ccgcaccagg tcgtccgcag cggctcggcc
     1801 gtccgcgagt tcgatcgaga gcatgccgcc gaagccgcgc atctgcgtct tggcccgctg
     1861 gtggccgggg tcggacggca ggcccgggta ccgcacccgg ctgatcgccg gatgctcgct
     1921 caaccggtcc gcgagcaccg cggcggaggc ctgttgccgc tcgagccgga cggccagcgt
     1981 tcgcagaccc cgcagggcca gccaggcctc catcgggccg agaatgcctc cgtgcaggcc
     2041 gcggtaggcc cgcagttgcg ccaacagcgc ctcgtcacgg acgaccagca ggcccagcac
     2101 cacgtcggaa tgaccggcga ggtacttggt gcccgagtgc atgacgaccg atgcgcccag
     2161 ttcgagcggg ttctgcagca gcggggtggc gaaggtgttg tcgaccgcga tgcgtacccc
     2221 caggcgggcg gcggccgcac cgagcgcgat caggtcggcg acctgcatca gcgggttcgt
     2281 cggcgattcg agcaccagcc agtcggcgcc gggcatggcc gccaccaccg cgtcggtgtc
     2341 gctgacgtcg accaggacca gcgcgaagcg tccggcgtcg gcgagctgct gggccaggga
     2401 caacatcgag ccgtagcacg agtcggccat caccacccgg ccgccctcgg gtaccaggct
     2461 gaacagcgcc gcacccgcag ccatgccggc cgcgaagcac agggccgtgc cccgctcgag
     2521 tgcgccgacg gcctcttcga gggccagcca ggtggcgttg ttcatccgtc catagatgcc
     2581 cgggtcggtc gcgccgaacg gctcccacga cacgtaggtc gacgtgagtt caaccggtgg
     2641 gctgactgcg gcaccggccc gatgcggcgg acgtccggcc acgacagcgg ccgtctcggg
     2701 cgaccaggac tgctcactca tgggtgctgc acctttcgct gagcggaagc gacgagccac
     2761 tggacgcatc ccacctcacg cgccgtattg caggcaaaac ggccgggtgc gtggccagtt
     2821 gaaagctgtg tcgatgacgt cgccacacta tcggccgcgt ctgaagccgc cgtcgctcac
     2881 cccttcttgg gacggtgctt gccgggcctg ggtgcccgcg gtagcggctc gcggtcgggc
     2941 agcgattcgc gcaccttctt gcgcagcact ttgcccagca tcgatgccgg cagctcgtcg
     3001 accacgtaga aggccctcgg caccttgtac ccggtcagcc gctgcttgca ccagctgcgc
     3061 aggtcggcgt cggtgagctg cgcgtcgggc tgcggcacca ccgcggccac cacccgttca
     3121 ccgcccacct cttcgttggg caggcccacc accgccgcgg aggcgacacc cggatgctcg
     3181 gacagcacct cttcgacctc ggacggcgag acgttgaacc ctccggtgat gatcaactcc
     3241 ttgacccggt cgacgatggt ggtgaagccg tcggcatcga cggtgaccag gtcgccggtg
     3301 cgcagccagc cgcccggcag cagcgtcttc tcggtctcgg ccggattgtt ccagtagccg
     3361 ctgaaaacct ggggtccctt gatcagcagt tcgcccggtt ggccctgcgg cacctcgacc
     3421 gtcggatcgg cggggtcgac caccttcatc caggtgctgg ggaacgggat gccgatggtg
     3481 ccggtgcggc gcgagggtgc gaacgggttg cccagacaga ccggcgaggc ctcggtcatg
     3541 ccgtaaccct cgaccagcag gccgcccgac accgactccc acaactgcac cgtggcggtg
     3601 ggcagagcca tcgcccccga gatgcagtac ttggccgaac gcagactgat gccgcgcttc
     3661 ttagcgctct tggcggtggc ctcatagatc ggcgggacgg cgcagtacac cgtgggtggg
     3721 ctcttcttcg cagcatccag caccatggtg acgtcgaagg acgggaacat caccagtcga
     3781 ccctgcttga ggatgccgta ggtcatgaac agggtcatgc cgaaggcgtg gaacatcggc
     3841 aggatcgcgt agaacacctc tttgcggtat tgggcgccgt gcatccaggc ctcgccctgc
     3901 cgggcgttgg cgaacaggtt gtagtggctg agcatcgcac ccttgggcag cgacgtggtg
     3961 cccgaggtgt actggatgac cgccagatcg tgcaccgacg gacgggggtg gcccgggtcg
     4021 agcaccggcg aggcgagtag tttctgccac gacacggtgc cgggtgccgg ctgggtcagc
     4081 ttgtcgcgca ggccacgcag cctcggcacc ggcagttgca gcgccaggcg tttcagggtc
     4141 gggaaggcct tcagcaggtt gaccgacacg atgtggtcgg gttcgagctc gaagtcgccg
     4201 tcgcgcagct tggccaccgc aacgtcccag gcgatcacca cgcgcgcgcc gtgatcatcg
     4261 aactggtggt gcaactcgcg tgcggtgtac agcggattgt gctcgaccac cactgcgccg
     4321 agccgcagca ccgcgtagaa ggcgacgatg tgctgcggac agttcggcag gatcagcgcc
     4381 acccgatcgc cggccgagac gccgagcgcc cgcaggccct cggccgctcg cgcgatctgc
     4441 tcgcccaact cggcgtaggt ggtctcgcgt ccgaagaact cggtggcgac cgcagagccg
     4501 ccttcggcga ccgacctctc atacaggctc accagtgact ccgtgggcaa ctcgatctcg
     4561 gccggcacgc cgggctgata gtgccgcgtc cacggcgcgg gcagcgtttc gctcatgcag
     4621 ccatcatttc ggtgattggc cccggatgca cccgtgccgg cccgtcaggg ctcgagtttg
     4681 ttgcgcgatg ggcgccggac gggtgaggtg ggtgccgacg cgtcacagca gcgcctcgag
     4741 cttgacgacc gtgttgaagt tgcgtccggt gaccaccagc ggcttgacca gggccggcag
     4801 cttcgcggcg agccttgagc ggccgatgcc gtcgacgaag tcgatccaca cctcgcgctc
     4861 gccgacctgg atgcgttcgt tgaccgcgac ctcggcaagc cgctcggcca gagcggacgg
     4921 tgccgcatcg ctgagaaagc agaccagcac gtgtgagggt gcaccatcgg ggaacggatt
     4981 ggcctcgatc agccgttgca gttggggctt gctgcgcagc agcacctcga cggtgaaccc
     5041 ggcgtcggcg gcgatcacct gttcgagact gctgcgcacg atcggctcgt cgccggttgc
     5101 cgtgaagacc aggttgccgg aggccagcag cgtggcgacc gcggtgaagc cggcgtctgc
     5161 cgcggcagcc ttgagttgcg cgttggtgag cgtgcggcca cccacgttga ccgcgcgcaa
     5221 caggccgacc cagcggctct cgttcgtcat ggcatcatcc tggacgatgc cactgacagt
     5281 gtgcgccggg gtctcgaccg gctcgaccag cgcgtgggtg atccggacca gctcgaccag
     5341 cgggtgacgg tggggcacga tgggcattcg gtgcagatga ggagggccag tgagcgtcac
     5401 cccgcactcc cccgtgccgt ccgcaactct tctgtccgca cccgtgctgt ccacacccgt
     5461 gctgtccaca cccgtgctgt ccgcacccgt gcttcccgag gccgaatggc ggccccgggc
     5521 gctggcccac gaccggcgcg tccgggcgtg gacggagccc tacctgcagc gccgctcgcg
     5581 ccgcgagccg cacccggtgg aggactttct gtggacgtac tactcctacc gtccgggcgc
     5641 gctgcgcacc tggcatccgg gctggggggc gacgctgagc ggtgacgtgg cggcgttggt
     5701 agccgttcgt ggcctgctcg tcgagcatga ccgggccgcg gtcgatccgg agctgccggc
     5761 ggcccgggcc gggcaggtcg aacagatccg ttcgctgctg gtggccacgc agtcccggcc
     5821 ggcccggctc ggctgcttcg gcctgcacga gtgggcgatg gtgtaccggc aatccgccga
     5881 gcaggtacga cacgcccagg tgccccttcg gctgggcgct gagggcaccg acgccgtggt
     5941 cgaggggcac cggatcacct gcacgcacta cgacgcctac cggttcttca ccgaggctgc
     6001 ggtgccgctc aacacgcgac gcccgacgct cgccacccgg gtactcaacg aacaaccggg
     6061 ctgcctgcac gccggcatgg acctctacaa gtgggcctac aagctggcgc cgatcacggg
     6121 tgccgagctg atcgcggact gcttcgagct cgctcgcgag atccgggtgc tcgacatgcg
     6181 cgcctcgccc tacgacctgc gcgagtgggg atacgccccc gtccgcatcg aaacccccga
     6241 tggcaaggcc gactacgtgc ggacgcagcg tgacttcagc acccgatccg gcgtcctgcg
     6301 caggcggctg atcgagctgt gcgacgccgt actggccgcc gcctgagcgc agtgtcagct
     6361 cagtcgctct cgacccactc cgccgccgcg acctcggccg ccgagggccc cttgatgtag
     6421 ctggtggcga ccagcgtgcc ctcgcgcgac acgttggtgt cggcggccat cgcgatgtcg
     6481 gtgttctggc agctgacgcc cagccaggtg ccgtccccga gcttgaccag ggtcgcgctg
     6541 atcaggcccc ggcgcgggtc gaccggctcg ttgtctgggt cgagctgtcc ggtgatctgc
     6601 cactgcgcca tcaccaccgc cgcgtcgtcg cccagaaacc gctggctgac cttgagcagc
     6661 ttgatggagg cgttcttgaa ggcgtgctga aagccgaacc gctgcgcgcg cacgatcgag
     6721 cggcgccccg tccaccacag gcccaccacg ttgacgaagt cggcgtcctc gacgaagagc
     6781 cgaccgatcg catcggcatc cttgtcgtcc caggctcgga cgtactcgtg aatcaggtcg
     6841 agcggcgtgg tgagcggatt caccgccggt gagcgggccg gaaacggcag tggggtgcgg
     6901 gcgaccgcgc gtttcagcag cttcttcagc ttggccacgt gtgctcctcg atcaggggac
     6961 tgctggcaag cgtgacgatg cggggcccga tcgtcaaccg ggtagcgccc gggcccctcc
     7021 agaaaagctg atccatcgct ggtcgaactc gtcgagactc gtgcgaccag ggggttcgag
     7081 ctcaaccaac gggtcgcaga tcagcgcttc cctacaccat caactggcgc tccgttggca
     7141 gaatcggctt gggcagccgg gagcgtccct gcaggtattg atcgacgccg ttggcgcacg
     7201 aacgtccctc ggcgatcgcc cagacgatca gcgactggcc gcgcccggcg tcgccgcaca
     7261 cgaacacgcc cggcaccgag gtggcgtagt tctcgtcccg agccacgttg ccgcgcgggt
     7321 cgagatcgac gcccaactgc tccaccagac ccttcttctc ggggcccacg aagcccatcg
     7381 ccagcagcac caggtcggcc ggaatctcgc gctcggtacc cggcaccg
//
LOCUS       DAOSUG010000083         7425 bp    DNA     linear   ENV 10-JUN-2024
DEFINITION  MAG TPA_asm: Micropruina sp. isolate AMR_MDS_2195
            NODE_4955_length_7425_cov_4.146676, whole genome shotgun sequence.
ACCESSION   DAOSUG010000083 DAOSUG010000000
VERSION     DAOSUG010000083.1
DBLINK      BioProject: PRJNA925477
            BioSample: SAMN32864262
            Sequence Read Archive: SRR4236673
KEYWORDS    WGS; ENV; Metagenome Assembled Genome; MAG; Third Party Data; TPA;
            TPA:assembly.
SOURCE      Micropruina sp. (wastewater metagenome)
  ORGANISM  Micropruina sp.
            Bacteria; Bacillati; Actinomycetota; Actinomycetes;
            Propionibacteriales; Nocardioidaceae; Micropruina.
REFERENCE   1  (bases 1 to 7425)
  AUTHORS   Abdulkadir,N., Saraiva,J.P., Zhang,J., Stolte,S., Gillor,O.,
            Harms,H. and Rocha,U.
  TITLE     Genome-centric analyses of 165 metagenomes show that mobile genetic
            elements are crucial for the transmission of antimicrobial
            resistance genes to pathogens in activated sludge and wastewater
  JOURNAL   Microbiol Spectr 12 (3), e0291823 (2024)
   PUBMED   38289113
REFERENCE   2  (bases 1 to 7425)
  AUTHORS   Abdulkadir,N.
  TITLE     Direct Submission
  JOURNAL   Submitted (24-MAR-2023) Environmental Microbiology, Helmholtz
            Center for Environmental Research -UFZ, Permoserstr 15, Leipzig
            04318, Germany
COMMENT     The annotation was added by the NCBI Prokaryotic Genome Annotation
            Pipeline (PGAP). Information about PGAP can be found here:
            https://www.ncbi.nlm.nih.gov/genome/annotation_prok/
            
            ##Genome-Assembly-Data-START##
            Assembly Method        :: SPAdes v. 3.11.1
            Genome Representation  :: Full
            Expected Final Version :: Yes
            Genome Coverage        :: Not Applicable
            Sequencing Technology  :: Illumina MiSeq
            ##Genome-Assembly-Data-END##
            
            ##Genome-Annotation-Data-START##
            Annotation Provider               :: NCBI
            Annotation Date                   :: 05/04/2023 04:57:10
            Annotation Pipeline               :: NCBI Prokaryotic Genome
                                                 Annotation Pipeline (PGAP)
            Annotation Method                 :: Best-placed reference protein
                                                 set; GeneMarkS-2+
            Annotation Software revision      :: 6.5
            Features Annotated                :: Gene; CDS; rRNA; tRNA; ncRNA
            Genes (total)                     :: 2,776
            CDSs (total)                      :: 2,750
            Genes (coding)                    :: 2,714
            CDSs (with protein)               :: 2,714
            Genes (RNA)                       :: 26
            tRNAs                             :: 24
            ncRNAs                            :: 2
            Pseudo Genes (total)              :: 36
            CDSs (without protein)            :: 36
            Pseudo Genes (ambiguous residues) :: 0 of 36
            Pseudo Genes (frameshifted)       :: 2 of 36
            Pseudo Genes (incomplete)         :: 32 of 36
            Pseudo Genes (internal stop)      :: 2 of 36
            ##Genome-Annotation-Data-END##
FEATURES             Location/Qualifiers
     source          1..7425
                     /organism="Micropruina sp."
                     /mol_type="genomic DNA"
                     /submitter_seqid="NODE_4955_length_7425_cov_4.146676"
                     /isolate="AMR_MDS_2195"
                     /isolation_source="Activated sludge of municipal
                     wastewater treatment plants"
                     /db_xref="taxon:2737536"
                     /environmental_sample
                     /geo_loc_name="Canada"
                     /lat_lon="49.25 N 123.1 W"
                     /collection_date="2015-06-16"
                     /metagenome_source="wastewater metagenome"
                     /note="metagenomic"
     gene            <1..549
                     /locus_tag="PLF56_04130"
     CDS             <1..549
                     /locus_tag="PLF56_04130"
                     /inference="COORDINATES: ab initio
                     prediction:GeneMarkS-2+"
                     /note="Derived by automated computational analysis using
                     gene prediction method: GeneMarkS-2+."
                     /codon_start=1
                     /transl_table=11
                     /product="hypothetical protein"
                     /protein_id="HOQ52781.1"
                     /translation="PCFDPTGSGTKQATMPLAKATDHTVWQDVAVEPQGYLYVLSYEG
                     VGASPDDYHLDLYRPDGSFLARTNEFAAAAIAVDIIRDVYSLNYEVLDAAAGPEPSIS
                     LWVPPVKSYRGSILSVDGASLVLQESPQTILTVSTSSDTVIRLNDVAATLADLKPGLT
                     LTAFGAMSSSGVLFATQLFATS"
     gene            560..2188
                     /locus_tag="PLF56_04135"
     CDS             560..2188
                     /locus_tag="PLF56_04135"
                     /inference="COORDINATES: protein motif:HMM:NF013003.2"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="pentapeptide repeat-containing protein"
                     /protein_id="HOQ52782.1"
                     /translation="MTILSISQFGFSAYLGAPVAPTGAVTGWTSVNADGRLDSVPNAS
                     STTSQVFNAYMNQLPPGTPGQGFALQSLTNGRYVVAATQSIAGTDRTVFHATGTTQDV
                     AAQLGLFQLGANLTYLWRNPADACWYPLINLDSGDGSGGGVVVASSTGYAGPYLAPWQ
                     SAQTNPAWSYTPPGGWTVPNGSDLSFVDFTCLNVTAMPFDFSRCRLKDTDLTGKTFGH
                     ALFVGCDLTAATLVPPLGAADDAWINLTGATVNYPSLGPDWRWLNLTRAVINQFPKPP
                     AKPPQINARGAVLDFVNMISWNLARADFTGASLCNANFNGSYLNGAVFHGAVLSPLTE
                     TPAAGPANFAYSYLFDADFSNAVAQGVSFAYAYLYGKAATVAGASLREADFANAFLPE
                     IDFSGVAEKDLVGVSFDGACLAGAGFQGTQLGKLHTKGFSFVGAALQGADFTGSNLDG
                     ANLTNAAVAATAPPPQGGLLKITAHYALGSINVPIEPITWTSATTLAPTDGTTFCPTG
                     TGPCQGAGLAPADPARSPQVAWPVVTARPAPPTI"
     gene            2237..3280
                     /locus_tag="PLF56_04140"
     CDS             2237..3280
                     /locus_tag="PLF56_04140"
                     /inference="COORDINATES: protein motif:HMM:NF019126.2"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="phage tail protein"
                     /protein_id="HOQ52783.1"
                     /translation="MSASTPVGTVLMYAGRTDGAGVLNLMLAGWILCDGSVYPNSQFP
                     DLFTVLGNSYGGNSSGFAVPQYKGLFQRGLDGNDPAQRDPGVATRTAPRPDLANVGNS
                     GNSVGSVQPDAFESHTHSYNYYNDYIESTHTLGHESLSGSTTSTTSSTGATETRPVNQ
                     YIDFIIKAVASPDVVPVGAVLPFAGDLATWAASLASAGWLPCTGSKQTPSSFPNLYST
                     VSNMFGADDQASFRLPDFRGRFLRGVVGQAIPGLPVADPDYLARTAPQPKLPYPGNTA
                     NQVGSLEPSTFAAHTHNYTWNNDYWSGAATAIGDHAETNAAVTWTTAANGSLTESRPI
                     NINVNYLIKAQNA"
     gene            3299..3823
                     /locus_tag="PLF56_04145"
     CDS             3299..3823
                     /locus_tag="PLF56_04145"
                     /inference="COORDINATES: protein motif:HMM:NF019126.2"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="phage tail protein"
                     /protein_id="HOQ52784.1"
                     /translation="MSSLYPVGSVLPYAGQFTPTSEAALIAQGYLPCDGRQLGTGDPQ
                     YQPLQKVIGNAYGGTKTTFCVPDYRGIFMRGTDGGAGVDPNAATRTPPQPSLAIPGNS
                     GDAVGSLQPGGLQGHTHTYASAGGDYHIINYGGAETPGVYAENKTASASTSASGGAET
                     RPRNASVNYVIAYL"
     gene            3837..5858
                     /locus_tag="PLF56_04150"
     CDS             3837..5858
                     /locus_tag="PLF56_04150"
                     /inference="COORDINATES: protein motif:HMM:NF020693.2"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="cholesterol oxidase substrate-binding
                     domain-containing protein"
                     /protein_id="HOQ52785.1"
                     /translation="MIQAQRIALTNAAWVILDCSVSAGPYRLSASSHLLRGDSATVDL
                     AGLPWPEGAVVLPVVEVEADIRHSPPPPPEPVQLTMNGCTAHYDVTGYTYNWTVTAGA
                     VDPPTPPPVLPGFPAQVPLNLLPYRNWDRQIVRAGMLTCAPRTPNDVAAVCNWAQDNA
                     YQVRVRGVMHGWSPLTLTQAPGNDDRVLLIDLTKGFAGMQLMSAGAGQPTRVKAGAGA
                     TMLELLTFLEQQPGGQGAAPGYSFPHTPAPGNLTVGGVLAIGAHGTAVPTPPADAFAA
                     SYGSISNQVTSLTVVATDPSDPGSRYAPRVLNRANPTTKAVLAHVGRALVLDATLQVI
                     DNYHLRCQSITDLPSATLFAAPSASDPVPANSFADFLERTGRLEIIWYPFSDNPWVHT
                     WQAAPTQPSGSIAVNHPFNYPFADVVPDGLQSLITSLVNGDPGLTPQVGAMAARVTAY
                     GLDGRNWAGVSGTYPVSRDIWGPSKNSLLYIQDTTLRVTANGYAIHLRRADVQQAVHD
                     VIGEYTSMLTGYAARGSYPVNSALELRVTGLDDPAAVGVAGAQSPAITALSMDAVDQA
                     NGWDVALWFDVLTIPGTSGADQFYGELETWLLGRFTGSAGRVLPEWSKGWAYTATGPW
                     TSTDFLTHVRSTLTQGRSSDDDWAWETATLKGLDAGGLFGNPFLDMLFG"
     gene            complement(5997..6935)
                     /locus_tag="PLF56_04155"
     CDS             complement(5997..6935)
                     /locus_tag="PLF56_04155"
                     /EC_number="3.1.2.-"
                     /inference="COORDINATES: protein motif:HMM:NF027439.2"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="enoyl-CoA hydratase/isomerase family protein"
                     /protein_id="HOQ52786.1"
                     /translation="MSDVIFDVQAGVGRITLNRPQAINALTTAMLADIGEQLSVWADE
                     PSVERVHLRGAGERGLCSGADVRALRADLLERGEVVAEVFFSVEYAVDALIATYPKPI
                     RADLFGITMGGGLGLSAHCADRVVRADSRLAMPETIIGLFPDVGVLYELSRVPGELGT
                     HLALTGGTVGAHDAVLVGLADRVEGEAPAGDLAWQRDWIDECYAGDDPLAIVQRLAEH
                     PDPQARATAEVLRQRCPFSVAVSLAAIRRAATLPDVASVLAQDLVLAVPMVLRPDFAE
                     GVRAQLVDKDRNPGWRPSRLEDVDAREVAALFADLG"
     gene            complement(6932..>7425)
                     /locus_tag="PLF56_04160"
     CDS             complement(6932..>7425)
                     /locus_tag="PLF56_04160"
                     /inference="COORDINATES: ab initio
                     prediction:GeneMarkS-2+"
                     /note="Derived by automated computational analysis using
                     gene prediction method: GeneMarkS-2+."
                     /codon_start=3
                     /transl_table=11
                     /product="hypothetical protein"
                     /protein_id="HOQ52787.1"
                     /translation="GWINALDVPGRVVTLAPLSLIGEGIRLLCVAAGRPELGTQIAGG
                     LLVAGVVASALIVAYLAVTVARHRPVTFLSWSYLWIALLGPAMHSWYLLWGGLLLPLT
                     RPTERMQRVAVGVTSVLLGYGAGNLAWRNDTGIALGLAGLALIITLLVRYFDEHRKSQ
                     GAT"
BASE COUNT         1233 a         2745 c         2372 g         1075 t
ORIGIN      
        1 ccctgcttcg acccgaccgg ctcggggacc aaacaggcaa ccatgccgct ggccaaggcg
       61 accgaccaca ctgtctggca ggacgtcgcc gtcgagccgc agggctacct gtatgtgctg
      121 tcctacgaag gtgtcggcgc ctcaccggac gactaccacc tcgacctgta ccgaccggac
      181 gggtccttcc tggcccgcac gaacgagttc gccgcggccg cgatcgcggt cgacatcatc
      241 cgcgacgtgt acagcctgaa ctacgaggtg ctggacgccg cggcaggacc ggagccgagc
      301 atcagcctgt gggtgccacc ggtgaagtcg taccggggca gcattctcag cgtcgacggc
      361 gcctcactgg tgctgcagga gtcaccgcag accatcctca cggtcagcac gagcagcgac
      421 accgtgatca ggctgaacga cgtggcggcg acgctggcgg acctgaagcc cgggctcacc
      481 ctcaccgcgt tcggagcgat gagttcgtcc ggcgtgctgt tcgccaccca actgttcgcc
      541 acgtcctgaa cggatcatca tgaccattct gagcatttcg cagttcggtt tctcggccta
      601 cctgggtgca ccggtcgcgc ccaccggagc ggtcacgggc tggacgtcgg tcaacgccga
      661 cgggcgcctc gacagcgtgc cgaacgcctc gtccaccacg agccaggtgt tcaacgccta
      721 catgaaccag ttgccgcccg gcaccccggg ccagggcttc gccctgcagt cgctgaccaa
      781 cggacgctac gtggtcgcgg cgacgcagag catcgccggc accgaccgca cggtgttcca
      841 cgccaccggc accacacagg acgtcgccgc ccagctgggc ctgttccagc tgggcgccaa
      901 cctcacctac ctgtggcgaa acccggccga tgcctgctgg tacccgctga tcaacctgga
      961 cagcggggac ggcagcggcg gcggcgtggt cgtcgcgtcc agcaccggct acgccggccc
     1021 gtacctcgca ccctggcagt cggcccagac caaccccgcg tggagctaca ccccgcccgg
     1081 cggctggacg gtaccgaacg gctccgacct gtcgtttgtg gacttcacct gcctcaacgt
     1141 caccgcgatg ccgttcgact tctcgcgctg ccggttgaag gacaccgacc tgaccggaaa
     1201 gaccttcggc cacgccctgt tcgtcggttg cgacctgacc gcagccaccc tggtgccccc
     1261 gctgggtgcc gcggacgacg cctggatcaa tctgaccggt gcgacggtga actacccgtc
     1321 gctgggtccg gactggcgat ggctcaacct gaccagggcg gtgatcaacc agtttccgaa
     1381 gccgcccgcc aagccgcccc agatcaacgc ccgcggcgcg gtgctcgact tcgtcaacat
     1441 gatcagctgg aacctggcca gggccgactt caccggagct tccctttgta acgcgaactt
     1501 caacggttcg tacctcaacg gagccgtctt ccacggcgcg gtgctctcgc cgctgaccga
     1561 gacgccggcg gcgggcccgg ccaacttcgc ctacagctac ctgttcgacg ccgacttcag
     1621 caacgcggtc gcccaaggcg tcagcttcgc ctacgcctac ctgtacggca aggctgcgac
     1681 cgttgccggc gccagcctgc gcgaggccga cttcgccaac gcgttcctgc ccgagatcga
     1741 cttcagcggc gtggccgaga aggacctggt cggggtctct ttcgacggtg cctgcctggc
     1801 cggtgcaggc ttccagggca cccagctggg caagttgcac accaaggggt tctcgttcgt
     1861 gggcgcagcc ctgcagggtg cggacttcac cggcagcaac ctggacggcg ccaacctgac
     1921 caacgcggcc gtcgcggcca ccgcaccgcc gccgcaaggc ggactgctca agatcaccgc
     1981 ccactatgcg ctgggctcca tcaacgtgcc gatcgaaccg atcacctgga cgtccgccac
     2041 cacgctggcc cccaccgacg gcaccacgtt ctgcccgacc gggaccggac cctgccaggg
     2101 cgccggcctg gctccggccg accccgccag gtcgccgcag gtcgcctggc ccgtggtcac
     2161 cgcccgtccc gctcccccga ccatctgaga atcgcgcccg cctttgtcaa ccatccccac
     2221 caacgaagga gaccccatgt ccgcctcgac ccccgtcggg accgtactga tgtacgccgg
     2281 ccgcaccgac ggtgccggcg tgctgaacct catgctcgca ggctggatcc tgtgcgacgg
     2341 ttcggtgtac cccaactctc agttccccga cctgttcacg gtgctcggca acagctacgg
     2401 cggcaactcc tcaggttttg cggtgccgca gtacaagggc ctgttccagc gcggcctgga
     2461 cggcaacgac cctgcccagc gcgaccctgg ggtcgcgacc cgcaccgcac cccgtccgga
     2521 cctggccaac gtgggcaaca gcggcaacag tgtgggctct gtgcagcccg acgcgttcga
     2581 atcgcacacg cactcgtaca actactacaa cgactacatc gagagcaccc acacgctggg
     2641 tcacgagtcc ctgtcgggct cgacgacgtc caccacgtcg tcgacgggcg ccaccgaaac
     2701 ccgccccgtg aaccagtaca tcgacttcat catcaaggcg gtggcttcgc cggacgtggt
     2761 gccggttggc gcggtactgc ccttcgcggg cgacctggcc acctgggccg catcgctggc
     2821 cagcgcgggc tggctgccgt gcaccggatc caagcagacc ccgtcgagct ttccgaacct
     2881 gtacagcacg gtgtcgaaca tgttcggcgc ggacgaccag gccagttttc ggttgcccga
     2941 ctttcgcgga cggttcctgc gcggcgtggt gggtcaggcg attcccggcc tgccggtcgc
     3001 cgacccggac tatctggccc gcaccgcgcc gcaacccaag cttccgtatc cgggcaacac
     3061 cgccaaccag gtcgggtcgc tggaacccag caccttcgcc gcacacaccc acaactacac
     3121 ctggaacaac gactactgga gcggtgcagc gacagcgatc ggcgatcacg ccgagaccaa
     3181 cgccgccgtc acctggacca ccgcagccaa tggctcgctg accgagagcc gcccgatcaa
     3241 catcaacgtc aactacctga tcaaggccca gaacgcctga acgcgaagga gaaccccgat
     3301 gtcctcgctg tatcccgtgg gatccgtgct cccctacgcc ggccaattca ctcctacttc
     3361 cgaggcggca ctgatcgcgc agggttatct gccttgcgac ggacgccaac tgggcaccgg
     3421 cgacccccag taccagccgc tgcaaaaggt gatcggcaac gcctacggcg gaacgaagac
     3481 cacgttctgc gtgcccgact accgcggcat cttcatgcgc ggaaccgacg gcggcgccgg
     3541 cgttgacccg aacgcggcca cccggacgcc gccccagccc agcctggcga ttcccggcaa
     3601 cagcggggac gccgtcggtt cgctgcagcc cggtgggttg cagggccaca cccacaccta
     3661 cgccagcgca ggcggtgact accacatcat caactacggc ggcgccgaga cgcccggggt
     3721 gtacgccgag aacaagaccg cctcggccag cacgtccgcg agcggtgggg cggagacccg
     3781 accgcgcaac gcgtccgtca actacgtgat cgcgtacctg tgagcgggca ccggtcatga
     3841 tccaggccca gcgcatcgca ctcaccaacg cggcgtgggt gatcctcgat tgcagcgtca
     3901 gcgccgggcc gtaccggctc agcgcatcaa gccacctgct caggggcgac tcggcaacgg
     3961 tggatctggc cggcctgccc tggccggagg gcgccgtcgt gctgccggtg gttgaggtgg
     4021 aggcggacat ccggcacagt cccccgccgc ctccggagcc ggtgcagctc acgatgaacg
     4081 gttgcaccgc gcactatgac gtcaccggct acacctacaa ctggacggtc accgcgggtg
     4141 cggtcgatcc gcccacaccg ccgccggtgc tccccggctt cccggcgcag gtgccgctga
     4201 acctgctgcc gtaccgcaac tgggatcgcc agatcgtccg tgcgggcatg ctgacctgtg
     4261 cgccgcgcac cccgaacgac gtggccgcgg tctgcaactg ggcgcaggac aacgcctatc
     4321 aggtgcgcgt gcggggcgtg atgcacggct ggtcgccgct gaccctcacg caggccccgg
     4381 gcaacgacga ccgggtgctg ctgatcgacc tgaccaaggg attcgccggc atgcagctga
     4441 tgtcggccgg agccgggcag ccgacccggg tgaaagcggg ggccggcgcc accatgctgg
     4501 agttgctcac ctttctggag cagcaacctg gcggtcaggg tgcggcgccc ggatactcgt
     4561 tcccacacac gcccgccccg ggtaacctca ccgtcggcgg cgtgctggcg atcggtgcgc
     4621 acggcaccgc ggtgcccacg ccacccgcgg acgccttcgc cgccagctac ggatcgatca
     4681 gcaatcaggt gacctcgttg accgtggtgg ccaccgatcc gtccgacccg ggtagtcgtt
     4741 acgcgccgcg cgtactgaat cgcgcgaacc cgaccaccaa ggccgtgctc gcccacgtcg
     4801 ggcgggcgct ggtgctggac gccacgctgc aggtgatcga caactaccac ctgcgctgcc
     4861 agagcatcac cgacctgccc agcgccacgc tcttcgcggc gccgagcgcg tccgacccgg
     4921 tgccggcgaa ctcgttcgcc gacttcctgg agcggacggg tcggctggag atcatctggt
     4981 acccgttcag cgacaatccg tgggtgcaca cctggcaggc ggcgccaacc cagccgtccg
     5041 gttcgatcgc cgtgaaccac ccgttcaact atccgttcgc cgacgtggtg ccggacggcc
     5101 tgcagtcgct gatcacgtcg ctggtcaacg gcgatccggg gttgaccccg caggtgggcg
     5161 cgatggccgc ccgggtgacg gcctacggtc tggacggccg caactgggcc ggggtctccg
     5221 gcacctatcc ggtgtcgcgt gacatctggg ggccgtccaa gaactcgctg ctctacattc
     5281 aggacaccac cctgcgggtg accgccaacg gttacgccat ccacctgcgc agggctgatg
     5341 tgcagcaggc cgtccatgac gtgatcggtg agtacacctc gatgctcacc ggctacgcgg
     5401 ctcgaggcag ctatccggtc aactccgcac tcgaactgcg ggtgaccggc ctggacgacc
     5461 ccgccgccgt cggtgtcgcc ggcgcccaga gcccggccat cacggcgctc agtatggacg
     5521 ccgtcgacca ggccaacggc tgggacgtgg ccctgtggtt cgacgtgctg accattcccg
     5581 gcacttccgg cgccgaccag ttctacggcg agttggagac ctggctgctc ggccgtttca
     5641 ccggcagcgc gggccgcgta ctaccggagt ggtcgaaggg ctgggcgtac accgccactg
     5701 ggccatggac ctcgaccgac tttctgaccc acgtgcgcag cacgctcacg caggggcgga
     5761 gcagcgacga cgactgggcg tgggagaccg ccacgctcaa gggcctggac gcgggcggcc
     5821 tgtttggcaa cccgttcctg gacatgttgt tcgggtgatt ctgagatcct gacaccagcc
     5881 ggcagctcgg tgcacgacac gccggggatc cccggaacta ctgcgggtga tttcagaacg
     5941 aggacacgca gggagcggcc cttgttggtc gagtcggtcg agaccgcggc gagtgcctag
     6001 ccgaggtcgg cgaacagtgc ggccacctcg cgggcgtcga cgtcttcgag gcgggacggg
     6061 cgccagcccg ggttgcggtc cttgtcgacc agttgggccc ggacaccctc ggcgaagtcc
     6121 ggacggagca ccatcggcac ggccagcacg aggtcctggg ccagcaccga ggccacgtcg
     6181 ggcagggtgg ccgcccgccg gatcgcggcc aggctgaccg caaccgagaa cgggcagcgc
     6241 tgccgcagca cctcggcggt cgcccgggcc tgcggatcgg ggtgctcggc cagccgctgc
     6301 acgatcgcca gtgggtcatc gccggcatag cactcatcga tccagtcacg ttgccaggcc
     6361 aggtcgccgg ccggggcctc accctcgacg cggtcggcca ggccgaccag caccgcatcg
     6421 tgcgcgccga cggtgccgcc ggtcagggcc aggtgcgtac cgagttcgcc cggcacccgc
     6481 gacagctcgt acagcacacc cacatcggga aagagcccga tgatggtctc gggcatggcc
     6541 agccggctgt ccgcgcgcac cacccgatcg gcgcagtgcg ccgacagccc gagcccgccg
     6601 cccatggtga tgccgaacag gtcagcccgg atcggtttgg ggtaggtggc aatcagggcg
     6661 tccaccgcgt actcgacact gaagaacacc tctgccacga cctcgccgcg ctccagcaga
     6721 tcggcccgca acgcccggac gtcggcgccc gaacacagcc cgcgctcgcc cgccccgcgc
     6781 agatgcaccc gctcgacgga cggctcgtcg gcccacacgc tcagctgctc accgatgtcg
     6841 gccagcatcg ccgtggtcag cgcgttgatc gcctgcggcc ggttcagtgt gatgcggccc
     6901 accccggcct gcacgtcgaa gatcacatcg ctcatgtcgc cccctgactc tttcggtgtt
     6961 cgtcgaaata gcgcaccagc agcgtgatga tcagcgccaa tccggccagg ccgagcgcga
     7021 tgccggtgtc attgcgccag gccaggtttc cggcgccgta gcccagcagc acgctggtga
     7081 cgcccacggc gacccgctgc atgcgctcgg tgggccgggt cagcggcagc aacaggccgc
     7141 cccacagcag gtaccacgag tgcatggcgg ggccgagcag ggcgatccac aggtacgacc
     7201 agctcaagaa cgtgaccgga cgatggcgcg ccaccgtcac cgccagatag gcgacgatca
     7261 acgcggacgc caccacgccg gccaccagca ggccgccggc gatctgggtg cccagctcgg
     7321 ggcgtccggc cgccacacag agcagccgga tgccctcgcc gatcagcgac agcggggcca
     7381 gggtgaccac ccggccgggc acgtccaggg cgttgatcca gccga
//
LOCUS       DAOSUG010000084         7421 bp    DNA     linear   ENV 10-JUN-2024
DEFINITION  MAG TPA_asm: Micropruina sp. isolate AMR_MDS_2195
            NODE_4963_length_7421_cov_3.470269, whole genome shotgun sequence.
ACCESSION   DAOSUG010000084 DAOSUG010000000
VERSION     DAOSUG010000084.1
DBLINK      BioProject: PRJNA925477
            BioSample: SAMN32864262
            Sequence Read Archive: SRR4236673
KEYWORDS    WGS; ENV; Metagenome Assembled Genome; MAG; Third Party Data; TPA;
            TPA:assembly.
SOURCE      Micropruina sp. (wastewater metagenome)
  ORGANISM  Micropruina sp.
            Bacteria; Bacillati; Actinomycetota; Actinomycetes;
            Propionibacteriales; Nocardioidaceae; Micropruina.
REFERENCE   1  (bases 1 to 7421)
  AUTHORS   Abdulkadir,N., Saraiva,J.P., Zhang,J., Stolte,S., Gillor,O.,
            Harms,H. and Rocha,U.
  TITLE     Genome-centric analyses of 165 metagenomes show that mobile genetic
            elements are crucial for the transmission of antimicrobial
            resistance genes to pathogens in activated sludge and wastewater
  JOURNAL   Microbiol Spectr 12 (3), e0291823 (2024)
   PUBMED   38289113
REFERENCE   2  (bases 1 to 7421)
  AUTHORS   Abdulkadir,N.
  TITLE     Direct Submission
  JOURNAL   Submitted (24-MAR-2023) Environmental Microbiology, Helmholtz
            Center for Environmental Research -UFZ, Permoserstr 15, Leipzig
            04318, Germany
COMMENT     The annotation was added by the NCBI Prokaryotic Genome Annotation
            Pipeline (PGAP). Information about PGAP can be found here:
            https://www.ncbi.nlm.nih.gov/genome/annotation_prok/
            
            ##Genome-Assembly-Data-START##
            Assembly Method        :: SPAdes v. 3.11.1
            Genome Representation  :: Full
            Expected Final Version :: Yes
            Genome Coverage        :: Not Applicable
            Sequencing Technology  :: Illumina MiSeq
            ##Genome-Assembly-Data-END##
            
            ##Genome-Annotation-Data-START##
            Annotation Provider               :: NCBI
            Annotation Date                   :: 05/04/2023 04:57:10
            Annotation Pipeline               :: NCBI Prokaryotic Genome
                                                 Annotation Pipeline (PGAP)
            Annotation Method                 :: Best-placed reference protein
                                                 set; GeneMarkS-2+
            Annotation Software revision      :: 6.5
            Features Annotated                :: Gene; CDS; rRNA; tRNA; ncRNA
            Genes (total)                     :: 2,776
            CDSs (total)                      :: 2,750
            Genes (coding)                    :: 2,714
            CDSs (with protein)               :: 2,714
            Genes (RNA)                       :: 26
            tRNAs                             :: 24
            ncRNAs                            :: 2
            Pseudo Genes (total)              :: 36
            CDSs (without protein)            :: 36
            Pseudo Genes (ambiguous residues) :: 0 of 36
            Pseudo Genes (frameshifted)       :: 2 of 36
            Pseudo Genes (incomplete)         :: 32 of 36
            Pseudo Genes (internal stop)      :: 2 of 36
            ##Genome-Annotation-Data-END##
FEATURES             Location/Qualifiers
     source          1..7421
                     /organism="Micropruina sp."
                     /mol_type="genomic DNA"
                     /submitter_seqid="NODE_4963_length_7421_cov_3.470269"
                     /isolate="AMR_MDS_2195"
                     /isolation_source="Activated sludge of municipal
                     wastewater treatment plants"
                     /db_xref="taxon:2737536"
                     /environmental_sample
                     /geo_loc_name="Canada"
                     /lat_lon="49.25 N 123.1 W"
                     /collection_date="2015-06-16"
                     /metagenome_source="wastewater metagenome"
                     /note="metagenomic"
     gene            <1..2756
                     /locus_tag="PLF56_04165"
     CDS             <1..2756
                     /locus_tag="PLF56_04165"
                     /inference="COORDINATES: ab initio
                     prediction:GeneMarkS-2+"
                     /note="Derived by automated computational analysis using
                     gene prediction method: GeneMarkS-2+."
                     /codon_start=3
                     /transl_table=11
                     /product="hypothetical protein"
                     /protein_id="HOQ52788.1"
                     /translation="AFSYLPEADRAGVNANSLAMLGEIIASQTKAAALSDGRLWSAFG
                     ASDIQSPVPFARVFPIGSMSGMAGRSARFGDGRSITIYRGLSRGLTALMMSPSAMGDF
                     VSYDLGNTEGLTFRHDRFGWGSEASQLVWGSMGFASVDMGRDRYAEYAAQRLARESVD
                     RLVRGHERKDSRASSQEQVQERAGAQFPYLLKSLQLWSGEGDFYEWFDSVAGWEKSAV
                     KVRARAIVTEVLVPHLPGPIAADGATWAAQVRQQLSGQARRLAEHGELEVYQWAFDYH
                     QALTARILSQISAGASRMGLPYAHQLVEQIGKHLVAISSHARIAEKRFADTSPDRLDD
                     KTEGLLGQLKRAVPNGQAVLDAVTRSATSRLSTRLWGRACGLLAQIWDDLGETFLAPL
                     SQALTRAFTDLDVAVKLTGRGVGLAVLETNEYSEWPTFERVPVRFDQATSEVLLTTSA
                     DYHKAFNEHVLTAIPGSRGSEDSLEDIKTAIISGHWESVGGSELPGELIELGRASRPQ
                     MLDHDPHTGQPLTPGPATFTVHATTAEIRSRARLFVDRPQSSFKNFCDESLREYLTPA
                     NAESAADQRERQARLTDCFVEALQLALPLSQPNDHVVREMHNVPVQYQYKFSAIPFAG
                     LPVAKRLRDRIVADTQVDHERVLGRYDEALDDLPVRRIQIFGSYPNYAPICFESVLEP
                     ARTQWRSATDVFSREPFWKWRRSRPLTGALPCSADERRAMVTGWFLGQLTGHLDLTGD
                     PIRVYSETRSTWLSLPHPLVTSSHWLQTHPEDLLPAVLESMLLAIANAGSTPDLSSLW
                     PYQTLRMLWDDSPMVAAPPGAASGVRLLSRWLQGETVPGGVSLLAVDPDASRATRTTA
                     VREFLSSKRAQIADLVKPVSSTDQLPELPLLYDLTDDLNWALTALEDAVSQSEWPARP
                     GVV"
     gene            2757..5315
                     /locus_tag="PLF56_04170"
     CDS             2757..5315
                     /locus_tag="PLF56_04170"
                     /inference="COORDINATES: ab initio
                     prediction:GeneMarkS-2+"
                     /note="Derived by automated computational analysis using
                     gene prediction method: GeneMarkS-2+."
                     /codon_start=1
                     /transl_table=11
                     /product="hypothetical protein"
                     /protein_id="HOQ52789.1"
                     /translation="MSAAVIVAAPSCLRSVLAVVMDWSASSLLTPMHWVEPDDHGQLT
                     VTNGLFVHNGRLTAEPIDAPLLRGQVDRVRLLVLVPTWKGAESISAAAEASVREAVRR
                     AAPGVQVTPIRVLLTVGDQFEDDSAPDVMGWHNVIVSPETSAQPGGFRQVHEHRDLIP
                     LVAASVASLGGLWTGVDTGPLDGQQTPPQGWPRLYRTQILWLDARRAEMAMRDELLHP
                     PEIPPPTRSTGAACAVLGAADVPVVTKDFADRLLSSHARLFESQRAQPAAEQTRTIGI
                     LDALGMFVTFLVSMFRPVDWLRARLARGKEVLASAVGDAVFGHDSRYVVKFGSGSLGQ
                     QHEALTRLESGLATRREISFESTGDFSGLWREFVDGALTLIDAGSRAGTLHPVPVGGS
                     RGVLPRTIDCAPTPGSGFVVDPALGLPRSSHQIPAWDRVELVSLDTVLGELADGEDAL
                     STIATRERARLREWVQQNQHSYAARVGSGLERRFRRVSTEIQELLARLTTKDDRVESQ
                     LAEQQRRLARQLQIILGIVVTGIVVTGVLGGVGLVEAATVGWLLGGLVVGWLAASLTT
                     FMLGQRALFRELERRRMLVSRREADEENLRKATFDLRRLVEAYRQFLGWAEVVSAVIA
                     EPFGARQNPRPAAGHRLADLPLNVRVAELSIDARQQSRAVALLQRSFFGTGWLSRPWE
                     STLADAGHRLGPDALSLIERPDRMFGEPGNADSRLIRWAAMLSREGVGSAPADEAWVD
                     VAARLVVSADELQLIENGVCIGPGGQAQAAADFAAARRVLAEQTGSPFDERVLTPAGV
                     VQGGNQVEWFEAPEHRHGLSRLTVRSDLGPGIEPHLLRRAGGSNGSTRRRDWFV"
     gene            5315..>7421
                     /locus_tag="PLF56_04175"
     CDS             5315..>7421
                     /locus_tag="PLF56_04175"
                     /inference="COORDINATES: ab initio
                     prediction:GeneMarkS-2+"
                     /note="Derived by automated computational analysis using
                     gene prediction method: GeneMarkS-2+."
                     /codon_start=1
                     /transl_table=11
                     /product="hypothetical protein"
                     /protein_id="HOQ52790.1"
                     /translation="MEGKGVTVGTELQRQAQDAALKLLRKQRALRMNDAVVGQLVGVP
                     VVAGRVASILRSAGLHALTNDVVALLEDAAARAVGGSGTGVTAREQPATTPVAGEKAS
                     GIPRSPAAATQPVAAASPQPIEEGHLADYTDPERLRYVLDTFAPDNSGAEAESTTTIE
                     RRSGRGGVALRWSDPAGAGEYSVFRLVTSDTAPPWSPDAAEADVVAVTTQLAYVDRRP
                     PITAVRHYQVWRNTGADHEAAVWGQPELIGEFSTVFAPTGFQVTERGNQVIGSWQALP
                     GTERVHVYRIPKSQLRLFRSDPGRFRIATEPAARNGFIDDGAKPGETYLYQIVAEASI
                     AAGQMQSATPQTAEFTGSATPQFIGDLTCQQPKNADEPVVNLEWTAQGRGSTRIYCTP
                     ARPTSGSDRSVLPVGELQRLGLPDDAALSHPVVQTDGRCSMERVGWPRGWGRMYLTPV
                     HIVGHEARVGGTVLQVLPPTLTALALHQRVDFQTITLAWPVQEGVDATRVGTLAADRV
                     SIFQAAPDVPVRAAIATSPLVTVSKEDYDRRGGIVLDHALRAQGSVVYAVPYAHAGTD
                     VVEGKPASVTYPGLLRIQTTLKITRRSVFKAGWAARVAFTADRDSPSPTFCVVHRADR
                     LPLAPEDGTHLPVVRAGIEGDVPSQRFQPSRLGSAPSDIFTVAIAEPGYVRVFALLGP
                     DRRRTAALLDPPIDDMRCGK"
BASE COUNT         1199 a         2444 c         2521 g         1257 t
ORIGIN      
        1 acgcgttcag ctacctgccc gaggccgacc gtgcgggtgt caacgcgaac tcgctggcga
       61 tgctgggcga gatcatcgca agtcaaacca aggctgccgc tctgtcggat gggcggctct
      121 ggtcggcctt cggcgccagt gacatccagt cgcccgtgcc cttcgccagg gtcttcccga
      181 tcggcagcat gtccggcatg gccggccgca gcgcgcggtt cggtgacggg cgttccatca
      241 ccatctaccg cgggctctcg cgtggcctga ctgcgctgat gatgtcaccg agcgcgatgg
      301 gtgatttcgt ctcgtacgat ctgggtaaca ccgaaggcct cacctttcgg catgatcgtt
      361 tcggctgggg cagcgaggcc agccagctgg tttggggcag catgggcttt gcctcggtcg
      421 acatgggcag ggacaggtac gccgagtacg ccgcgcagcg gcttgctcgc gaaagcgtgg
      481 accggctggt ccgcggccat gagcgcaagg acagcagggc ctcctcgcag gaacaggtgc
      541 aggaacgtgc cggtgcgcag ttcccctatc tgctcaagag cctgcagttg tggagcggag
      601 agggcgactt ctacgagtgg ttcgacagcg tcgctgggtg ggagaagtcc gcagtcaagg
      661 tacgcgcccg ggcgatcgtc accgaagtgc tcgtgccgca cctgcccggt ccgatcgcgg
      721 cggacggcgc gacctgggcc gcccaggtgc gtcagcagtt gagcggccag gcacgacgac
      781 tggcggaaca cggtgagctc gaggtctacc agtgggcgtt cgactaccac caggcgctga
      841 cggcacggat cctcagccag atcagcgccg gcgccagccg gatgggactg ccgtacgctc
      901 accagctcgt cgagcagatc gggaagcatc tggtcgcgat cagcagccac gcacgcatcg
      961 ccgaaaagcg cttcgccgac acgtcgcccg accggctcga tgacaagacc gaaggcctgc
     1021 tcggtcagct gaaacgcgcc gtccccaacg gccaggccgt tctggacgcc gtgacccgca
     1081 gtgcgacgtc acggttgtcg acccggttgt ggggtcgcgc ctgcggactg ctcgcgcaaa
     1141 tctgggacga cctgggtgag accttcctgg ctcccctctc gcaggcgttg accagggcgt
     1201 tcacagactt ggacgttgcg gtcaaattga ccggtcgggg ggtggggctg gccgttctcg
     1261 agaccaacga gtattccgaa tggccaacct tcgaacgggt gcccgtgcgc tttgatcagg
     1321 cgaccagtga ggtgctgctc accacttcgg ccgactacca caaggccttc aacgagcatg
     1381 tgctgaccgc gatccccggc tcgcggggat cagaggattc gctggaggac atcaagaccg
     1441 ccatcatctc gggtcactgg gagagtgtgg gcggtagcga actgccgggc gagctcatcg
     1501 agctcgggcg ggcctcgcgt ccgcagatgc tcgaccacga tccgcataca ggccagccgc
     1561 tcacccccgg ccccgccacg ttcacggttc acgccaccac tgccgagatc cgcagccggg
     1621 cacggctgtt cgttgatcgg ccacagtcgt cgttcaagaa cttctgtgac gagtcgctcc
     1681 gcgagtacct gactccggcg aacgcagaga gtgcggccga ccagcgcgaa cggcaggcgc
     1741 gcctgaccga ctgcttcgtg gaagcgctgc agttggccct gccgctgtca cagcccaacg
     1801 atcacgtcgt ccgcgaaatg cacaacgtgc cagtccagta ccagtacaag ttctcggcca
     1861 tcccctttgc cggattgccg gtcgcgaagc gcctgcgcga ccggatcgtg gccgacaccc
     1921 aggtggacca cgagcgcgtg cttgggcgct acgacgaggc actggacgac ctgccggtgc
     1981 gccgcatcca gatcttcggc tcgtacccca actacgcccc gatctgcttc gagagcgtcc
     2041 tggagccggc ccgaacccaa tggcgatccg ccactgacgt cttcagccgc gaacccttct
     2101 ggaagtggcg ccgtagtcgt cccctgactg gggcattgcc ctgttcggcc gacgagcgcc
     2161 gcgcgatggt gacgggctgg ttcctcggcc agctgaccgg gcacctcgac ctcacgggtg
     2221 acccgattcg ggtctactcc gagacgcgca gtacctggct cagcctcccg caccccttgg
     2281 tcacatcgag tcattggctg cagacccacc ccgaggacct gttgccggcc gtgctggagt
     2341 ccatgctgct ggccatcgcc aacgccggca gcactcccga cctgtcgtcg ctgtggcctt
     2401 accagacgct acggatgctt tgggacgact ctccgatggt ggccgcgcct cccggggcag
     2461 cgagcggcgt ccggctgctg agtcgttggc tgcaggggga aaccgtgccg ggtggtgtct
     2521 cgctgctggc cgtggacccg gacgcctcgc gggcgacccg tacgaccgcc gtccgcgagt
     2581 tcctcagcag caagcgcgcg cagatcgcag acctcgtcaa gcccgtctcg tcaacggacc
     2641 agttgcccga gctgccgctg ctctatgacc tgaccgacga cctgaactgg gccctgactg
     2701 cgttggagga tgcggtcagc cagagtgaat ggccggcacg ccccggggtg gtctaggtgt
     2761 ccgctgcagt gatcgtcgca gcgccgtcct gcctgcgctc agtcctcgcc gtcgtgatgg
     2821 actggtcggc cagcagcctg ctgaccccga tgcactgggt ggaacctgat gatcatggcc
     2881 agctgaccgt gaccaacggg ctgttcgtgc acaacggtcg cctgacggcg gaaccaatcg
     2941 atgccccgct actacgtgga caggtggatc gggttcgtct gctggtgctg gtgccgacgt
     3001 ggaagggtgc ggagtcgata agtgcggcgg ctgaggcttc ggtccgggag gcagtccgtc
     3061 gcgcggcgcc tggggtacag gtgacgccca tacgcgtcct gctcacggtg ggcgatcagt
     3121 tcgaggacga ctcggcgccg gacgtgatgg gctggcacaa cgtcatagtt tcgccagaaa
     3181 cctcggcgca gccgggtggc ttccgacagg tacacgagca tcgcgacctc atacccctgg
     3241 tggctgcgag cgtggccagc ctgggcgggc tctggaccgg tgttgatacg ggtccactcg
     3301 atggtcagca aacgcccccg cagggttggc cgcgactgta ccgcacccag atcctttggc
     3361 tggacgcccg tcgagccgag atggcaatgc gcgatgaact gctgcacccg cccgagatcc
     3421 cgccgcccac ccggtcgacg ggcgccgcgt gcgcagtcct cggggccgca gatgtccccg
     3481 tggtgaccaa ggattttgcc gatcggctac tgtcctcgca cgcgagactc ttcgagagcc
     3541 aacgcgccca gccggccgcc gagcagacca gaacgatcgg cattctggat gcgctcggca
     3601 tgttcgtcac cttcctcgta tcgatgtttc gtcccgttga ctggttgcgc gcgcgactcg
     3661 ctcggggcaa agaggtgttg gcgtccgcgg tcggagatgc tgtgttcggc catgacagca
     3721 ggtacgtggt caagttcggc agcgggtcgc tcggtcagca gcatgaggca ctcacccggc
     3781 tcgagtctgg gttggcgacc cggcgcgaaa tttccttcga gagcacggga gatttctcgg
     3841 ggctgtggcg cgagttcgtc gacggtgccc tcaccctgat cgacgctggt tcgcgggccg
     3901 ggaccctgca tccggtgccg gtgggagggt cgcgaggcgt cctgccccgg accatcgact
     3961 gcgcgcccac tcccggtagc ggtttcgtag tcgatccagc gctgggtttg ccgcgcagct
     4021 cgcaccagat ccccgcatgg gatcgcgtcg aactggtttc gctggacacc gtactgggag
     4081 agctggcgga cggggaggac gcgttgtcga cgatcgcgac ccgcgagcgt gcgcggctgc
     4141 gggaatgggt gcagcagaat cagcactcgt acgcggcgcg cgtgggcagt ggtctggagc
     4201 gccggttccg cagagtgagt accgagatcc aggaactgct ggcccgcctg acgaccaagg
     4261 atgaccgcgt cgagagccag cttgcagagc agcagcgcag gctggcgaga caactgcaga
     4321 tcatcttggg cattgtcgtc accggcattg tggtcaccgg agtgctgggc ggggttggcc
     4381 tcgtggaggc tgccaccgtc ggctggctgc tgggcgggct ggtcgtcggg tggctcgccg
     4441 cctcgctgac gactttcatg ctggggcagc gggcgttgtt tcgtgaactc gaacgccggc
     4501 gcatgctggt ttcccgtcgt gaggccgatg aagagaacct gcgcaaggcc acgttcgatc
     4561 tgcgtcgctt ggtcgaggcg tatcgacagt tcctcggttg ggccgaggtg gtctccgctg
     4621 tgatagccga gccgttcggt gctcggcaga atccgcgtcc cgccgcgggg caccggctcg
     4681 ctgacctgcc gctcaacgtt cgggtcgcag aactgtcgat cgatgcgcgc caacagtccc
     4741 gtgccgtggc tctcttgcaa aggtcgttct ttggtaccgg ctggctctcc aggccctggg
     4801 agtcgaccct ggccgatgcg gggcatcggc tcggcccgga cgcgctgtcg ctcattgagc
     4861 gaccagaccg gatgttcggc gagcccggaa acgccgactc ccgtctcatc cggtgggctg
     4921 cgatgctgtc ccgtgaggga gtcggcagcg ctcccgcgga cgaggcctgg gtcgacgtcg
     4981 ccgcccggct tgtcgtcagt gccgacgagt tgcagttgat agagaacggt gtctgcatcg
     5041 gccccggggg gcaggcccag gccgcagccg atttcgcggc agcgcgcagg gtcctcgccg
     5101 agcagactgg aagcccgttc gatgagcgcg tgctcacccc cgccggcgtc gttcaggggg
     5161 gcaaccaggt tgaatggttc gaggcgcccg agcacaggca cggactgtct cggctgaccg
     5221 tgcgaagcga tctaggaccc ggcatcgaac cgcacctgct gcgccgtgcc gggggcagca
     5281 acggcagcac ccggcgacgc gactggttcg tctgatggaa ggcaagggcg tgactgtggg
     5341 cactgagctt cagcgccaag cgcaggatgc ggcgctgaaa ctgctgcgga agcagagggc
     5401 gctcagaatg aacgatgccg tcgtcggtca gttggtgggc gtgcccgttg tcgctggacg
     5461 tgtggcgtcg atcctgcggt ccgccggcct tcatgccctg accaacgacg tggtcgcgtt
     5521 gctcgaggat gcggcagcgc gggcggttgg cggctccggg acgggcgtaa ctgcccgcga
     5581 gcagccggcc accaccccgg tagccggcga aaaggcgtct ggcattccac gctcgccggc
     5641 ggccgccaca cagccggtag cggccgcgtc accccaacca atcgaagagg ggcacctggc
     5701 cgactacacc gacccggaac ggctccgata cgtactggac acatttgccc ctgacaacag
     5761 cggtgccgag gccgagtcca cgaccacgat cgaaagaaga tccggtaggg gcggggtggc
     5821 actgcgctgg tcggatcccg ccggcgcggg cgagtactcc gtgttccggc tggtgaccag
     5881 cgacacggcc ccgccctggt cgccggacgc ggcggaggcc gacgtcgtgg cggtgaccac
     5941 ccagctcgcc tatgtcgatc ggcgtccacc tatcactgcg gttcggcact accaggtgtg
     6001 gcgcaacacc ggcgccgatc acgaggcagc ggtgtggggg cagccggagc tcatcggcga
     6061 gttcagcacg gtatttgcac ccaccggatt ccaggtcacc gaacgcggca atcaggtgat
     6121 cggctcgtgg caggcacttc caggcaccga gcgcgtacac gtctacagga tcccgaagag
     6181 ccagctgcgg ctgttccgct ccgacccggg ccgattccgc attgccaccg agcctgccgc
     6241 tcggaacgga ttcattgatg acggagccaa gcctggcgaa acctaccttt accagatagt
     6301 ggcagaagcc tcgatcgctg cgggacagat gcagtccgcc accccgcaga ctgctgagtt
     6361 cacagggtct gccaccccgc agttcatcgg ggatctgacc tgccagcagc cgaagaacgc
     6421 cgacgagccg gtggtgaatc ttgaatggac cgcgcagggc cggggaagca cccggatcta
     6481 ctgcacgccg gcacgcccca cctcgggcag cgaccgcagt gtgctgcccg tcggggaact
     6541 gcaacgactg ggcctgccag atgacgcagc gctgagccac cccgtcgtcc agaccgacgg
     6601 ccggtgctcg atggagcggg tcgggtggcc gcgtggctgg ggacgcatgt acctcacgcc
     6661 cgttcacatc gtgggacacg aggcgcgagt cggtggcacc gtacttcaag tactcccgcc
     6721 cacgctgacg gccctggccc tgcatcaacg ggtcgacttt cagaccatca ccttggcgtg
     6781 gcctgttcaa gaaggcgtgg acgcgacccg cgtcggcacc ctggccgcag atcgggtgag
     6841 catcttccag gctgcgcctg atgtgcccgt acgggcggcc atagcaacat ctcccctggt
     6901 cacggtgtcc aaggaggatt atgaccggcg ggggggaatc gtactcgacc acgctctgcg
     6961 cgcccaggga tcggttgtct acgcggtgcc gtacgcgcac gctggtaccg acgtggtcga
     7021 aggtaagccc gcgtccgtga cctatccggg actacttcgg atccagacga ctctgaagat
     7081 cacccgccgc tcggtgttca aggccggctg ggccgcccgg gtcgcgttca cggcggaccg
     7141 cgattcgccc tcaccgactt tctgtgtggt gcaccgggcg gatcggcttc ccctagcccc
     7201 tgaagatgga acccatctgc ccgtggtgcg ggcaggcatc gaaggtgacg tgccgagcca
     7261 gcgcttccaa ccgtccagac tcggcagcgc ccccagcgac atcttcaccg tggccattgc
     7321 cgagcccgga tacgtcagag tgttcgccct cctggggccg gatcggcggc gaacagctgc
     7381 tttgctggac ccgcccatcg acgacatgcg gtgcggaaaa t
//
LOCUS       DAOSUG010000085         7397 bp    DNA     linear   ENV 10-JUN-2024
DEFINITION  MAG TPA_asm: Micropruina sp. isolate AMR_MDS_2195
            NODE_4978_length_7397_cov_4.503950, whole genome shotgun sequence.
ACCESSION   DAOSUG010000085 DAOSUG010000000
VERSION     DAOSUG010000085.1
DBLINK      BioProject: PRJNA925477
            BioSample: SAMN32864262
            Sequence Read Archive: SRR4236673
KEYWORDS    WGS; ENV; Metagenome Assembled Genome; MAG; Third Party Data; TPA;
            TPA:assembly.
SOURCE      Micropruina sp. (wastewater metagenome)
  ORGANISM  Micropruina sp.
            Bacteria; Bacillati; Actinomycetota; Actinomycetes;
            Propionibacteriales; Nocardioidaceae; Micropruina.
REFERENCE   1  (bases 1 to 7397)
  AUTHORS   Abdulkadir,N., Saraiva,J.P., Zhang,J., Stolte,S., Gillor,O.,
            Harms,H. and Rocha,U.
  TITLE     Genome-centric analyses of 165 metagenomes show that mobile genetic
            elements are crucial for the transmission of antimicrobial
            resistance genes to pathogens in activated sludge and wastewater
  JOURNAL   Microbiol Spectr 12 (3), e0291823 (2024)
   PUBMED   38289113
REFERENCE   2  (bases 1 to 7397)
  AUTHORS   Abdulkadir,N.
  TITLE     Direct Submission
  JOURNAL   Submitted (24-MAR-2023) Environmental Microbiology, Helmholtz
            Center for Environmental Research -UFZ, Permoserstr 15, Leipzig
            04318, Germany
COMMENT     The annotation was added by the NCBI Prokaryotic Genome Annotation
            Pipeline (PGAP). Information about PGAP can be found here:
            https://www.ncbi.nlm.nih.gov/genome/annotation_prok/
            
            ##Genome-Assembly-Data-START##
            Assembly Method        :: SPAdes v. 3.11.1
            Genome Representation  :: Full
            Expected Final Version :: Yes
            Genome Coverage        :: Not Applicable
            Sequencing Technology  :: Illumina MiSeq
            ##Genome-Assembly-Data-END##
            
            ##Genome-Annotation-Data-START##
            Annotation Provider               :: NCBI
            Annotation Date                   :: 05/04/2023 04:57:10
            Annotation Pipeline               :: NCBI Prokaryotic Genome
                                                 Annotation Pipeline (PGAP)
            Annotation Method                 :: Best-placed reference protein
                                                 set; GeneMarkS-2+
            Annotation Software revision      :: 6.5
            Features Annotated                :: Gene; CDS; rRNA; tRNA; ncRNA
            Genes (total)                     :: 2,776
            CDSs (total)                      :: 2,750
            Genes (coding)                    :: 2,714
            CDSs (with protein)               :: 2,714
            Genes (RNA)                       :: 26
            tRNAs                             :: 24
            ncRNAs                            :: 2
            Pseudo Genes (total)              :: 36
            CDSs (without protein)            :: 36
            Pseudo Genes (ambiguous residues) :: 0 of 36
            Pseudo Genes (frameshifted)       :: 2 of 36
            Pseudo Genes (incomplete)         :: 32 of 36
            Pseudo Genes (internal stop)      :: 2 of 36
            ##Genome-Annotation-Data-END##
FEATURES             Location/Qualifiers
     source          1..7397
                     /organism="Micropruina sp."
                     /mol_type="genomic DNA"
                     /submitter_seqid="NODE_4978_length_7397_cov_4.503950"
                     /isolate="AMR_MDS_2195"
                     /isolation_source="Activated sludge of municipal
                     wastewater treatment plants"
                     /db_xref="taxon:2737536"
                     /environmental_sample
                     /geo_loc_name="Canada"
                     /lat_lon="49.25 N 123.1 W"
                     /collection_date="2015-06-16"
                     /metagenome_source="wastewater metagenome"
                     /note="metagenomic"
     gene            <1..1262
                     /locus_tag="PLF56_04180"
     CDS             <1..1262
                     /locus_tag="PLF56_04180"
                     /inference="COORDINATES: protein motif:HMM:NF021619.2"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=3
                     /transl_table=11
                     /product="PocR ligand-binding domain-containing protein"
                     /protein_id="HOQ52791.1"
                     /translation="SGATGLATVAVDARGIPVTPPCAFTDFCTQMRSDPARNSLCHGC
                     DAHGGLQSLIEGAPKLYRCHSGLVDFSVPVTEGDRYVGAILCGQVRVPGVDQPDFLIA
                     PTAWRGEQPLEERYQEVTVSSLRKVRAAATTLLGLSRTIGRSKAARSTDLQQAAQSEP
                     QLPHLTLVPVDEVLTPVSAAKEHPVDAAQERLTLLRDAMNAEDLATAFVVTARVLDEV
                     HALPADHVREGLAELEDVLIEVARHCAPRLVPHLSGMVQRQRDRRTSSPNRYKSQLYA
                     ERILGMVLDEIIRCRPHRPRDLRDLLNDIARRPNRALSLTEAAAFTHWSPGYLSKMFK
                     SVTGCTFVSYLMQWRISRAKLMLASTQMPVRKIAAELDFNQVNYFSRVFRAHTGHTPS
                     EYRRSTSREERPFDASLSAHHHTVLRA"
     gene            1219..2349
                     /locus_tag="PLF56_04185"
     CDS             1219..2349
                     /locus_tag="PLF56_04185"
                     /EC_number="1.1.1.1"
                     /inference="COORDINATES: protein motif:HMM:NF012678.2"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_function: GO:0016491 - oxidoreductase activity
                     [Evidence IEA];
                     GO_function: GO:0046872 - metal ion binding [Evidence
                     IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="iron-containing alcohol dehydrogenase"
                     /protein_id="HOQ52792.1"
                     /translation="MHRFQLTTTLCYGPDALSALGALAERRVLLVTDAFLAAGDLMAL
                     VRRELRDAEVKVFDAVRPNPDIAAVTEGLRSFLAFSPDAIVALGGGSPIDTAKAVRKL
                     ALEQGHDLPAGLVVMPTTSGSGSEVTAFAVVTDAESHAKLPLTSVDMLPEVAILDAEA
                     VLTAPPRLTADSGMDAVSHAIEAYVAKGANVFSDALAEKALRLADAALVRCFVDGSDA
                     QAREQQHAAATMAGIAFQNAGLGIVHGLSHAIGGSFKVAHGRLNGILLPHVIAFNAGP
                     LGLRPHELSDVARRYAALARAMGLQASNERNLVLALSGWVERLRRDLAMPATVTAAGV
                     GRAEFLAAVPALAVTALTDFCTSGNPVDVSEADLTGILRAVV"
     gene            complement(2355..2780)
                     /gene="eutP"
                     /locus_tag="PLF56_04190"
     CDS             complement(2355..2780)
                     /gene="eutP"
                     /locus_tag="PLF56_04190"
                     /inference="COORDINATES: protein motif:HMM:TIGR02528.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_process: GO:0046336 - ethanolamine catabolic process
                     [Evidence IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="EutP/PduV family microcompartment system
                     protein"
                     /protein_id="HOQ52793.1"
                     /translation="MKKILLVGSVGCGKTTLLQRLHGDEISYSKTQTIYTDGQTIDTP
                     GEYLELPWFKHSLRMASFDAELVVLLASATVNESKFPPGFTTFFMPPSIGVVTKVDIA
                     EPRQVSLAGDYLRLAGALEVFEVSAITGEGIEALEARLR"
     gene            complement(2777..3121)
                     /gene="eutS"
                     /locus_tag="PLF56_04195"
     CDS             complement(2777..3121)
                     /gene="eutS"
                     /locus_tag="PLF56_04195"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_013160807.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="ethanolamine utilization microcompartment
                     protein EutS"
                     /protein_id="HOQ52794.1"
                     /translation="MADDKQRIIQEFVPGKQVTLAHVVANPDEKLFPKIGLPPQGGAI
                     GVMTITPSEAAIIAADIATKSADVELGFVDRFSGSLVVIGRLVDVQTAVHSVVDGLCD
                     NLGFARPPLTST"
     gene            3404..3682
                     /gene="pduA"
                     /locus_tag="PLF56_04200"
     CDS             3404..3682
                     /gene="pduA"
                     /locus_tag="PLF56_04200"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_014847888.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="propanediol utilization microcompartment protein
                     PduA"
                     /protein_id="HOQ52795.1"
                     /translation="MQEALGMVETKGFVAAVEAADAMVKSANVVLIGTQRVGSGLVTV
                     LVRGDVGAVKAATDAGAVAAQNVGELVSVHVIPRPHTDVEKILPSTEK"
     gene            3695..4489
                     /gene="pduB"
                     /locus_tag="PLF56_04205"
     CDS             3695..4489
                     /gene="pduB"
                     /locus_tag="PLF56_04205"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_013160809.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_component: GO:0031469 - bacterial microcompartment
                     [Evidence IEA];
                     GO_function: GO:0005198 - structural molecule activity
                     [Evidence IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="propanediol utilization microcompartment protein
                     PduB"
                     /protein_id="HOQ52796.1"
                     /translation="MASEDLVNQIMAAVMKKIDSPAAAAAPTKTASGARPASTEFVGC
                     SPIGDTIGLVIANVDPQIHDLMKIDGKYRSIGIVGARTGAGPHIFAADEAVKATNTEV
                     LTIELPRDTKGGAGHGSLIIFGAEDVSDARRAVEVTLSEIERTFGDVYANDAGHLEFQ
                     YTARASLALNKAFGAPLGKAFGITVGAPAAIGVVLADTAVKASTIDVIGYSSPAAGTS
                     FSNEVISFFSGDSGAVRQAIIAAREVGRGLLGAMGDYPESATTPYI"
     gene            4504..6165
                     /locus_tag="PLF56_04210"
     CDS             4504..6165
                     /locus_tag="PLF56_04210"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_013160810.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="propanediol/glycerol family dehydratase large
                     subunit"
                     /protein_id="HOQ52797.1"
                     /translation="MKSKRFEALDDRPVNKDGFVTEWPEVGLIAMNGPNDPQPSIRIQ
                     NGKVVELDGKSRDEFDMIDTFIADYGIKLDNAEVVNAMDSLELARKLVDINVPRAEVV
                     KLTTAMTPAKICEVIGKMTVLEMMMSVTKMRARKSPANQAHVTNLKDNPVQIAADAAE
                     ASLRGFAELETTVGIARYAPFNAIALMVGAQVGRPGILTQCAVEEATELNLGMRGFTA
                     YAETVSVYGTEPVFMDGDDTPWSKAFLASCYASRGLKMRFTSGTGSEVQMGYAEGKSM
                     LYLEARCLYITKAAGSQGIQNGSVSCVGVPAAVPGGIRAILAENLIATALDLECASSN
                     DQTFTHSDLRRVARSLMQFVPGTDFICSGYSATPNYDNMFAGSNWDADDYDDWIIIQR
                     DLKVDGGLVPVLEEDVVRVRNKAAKVIQAVFRELGLTEVTDEEVEAATYARGSQDMPD
                     RNVVEDLKAAEDLLKRGLTGVDIVKALAKSGFEDVASFVYGMLRQRVAGDYLHTSAIL
                     NDKFEVISAVNQPNDYNGPQTGYQISDEKWELLKTIRQAISPENV"
     gene            6176..6841
                     /locus_tag="PLF56_04215"
     CDS             6176..6841
                     /locus_tag="PLF56_04215"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_016456621.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="propanediol/glycerol family dehydratase medium
                     subunit"
                     /protein_id="HOQ52798.1"
                     /translation="MDEKTLRSIIEEVMKEFGAGSGGGTATAVMTAPSVSSASGTLSI
                     SEAGPADRGTNPREVVIALAPGFGGRMTSTITGIDHGEVLREVCAGIEEQGLTYRFIR
                     VFRTADVGFIAHEAAKLSGSGIGIGIQSRGTTVIHQKDLPPLSNLELFSQAPLIDLDT
                     FRAIGENAAKYAKGESPSPVPVRNDQMARPRYQAIAALWHIKETQLCDPHKKTMTLRV
                     EYR"
     gene            6851..7369
                     /locus_tag="PLF56_04220"
     CDS             6851..7369
                     /locus_tag="PLF56_04220"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_014847892.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="diol dehydratase small subunit"
                     /protein_id="HOQ52799.1"
                     /translation="MESEELIRQIMDEVMKNLAKDQVSFAKKPAANGSAGSVSAANYP
                     LGEKMPEKIKSATGKVLSDFSAEKVISGQLTAEDFRIAPETLELQAQVAESVGRDTLA
                     RNMRRAAELIKVPDDELLQVYNSLRPYRSTKAELYGIADGLESKYGCTVNAAFIREAA
                     DVYEKRGRLKAD"
BASE COUNT         1292 a         2537 c         2421 g         1147 t
ORIGIN      
        1 tctcgggcgc gaccgggctg gccacggtcg cggtcgacgc ccgcggcata ccggtcaccc
       61 cgccctgtgc cttcaccgac ttctgcaccc agatgcgctc cgatccggcc cgcaactcgc
      121 tgtgccacgg atgcgacgcc catggcggtc tacagtcgct gatcgagggc gcccccaagc
      181 tgtaccgctg tcattcgggg ctggtcgact tctcggtgcc ggtcactgag ggtgaccgct
      241 acgtgggcgc catcctgtgc ggtcaggtgc gggttccggg tgtcgaccag ccggattttc
      301 tgatcgcccc gaccgcgtgg cgcggcgagc agccgctgga ggagcgatac caggaggtca
      361 ccgtgtcgag cctgcgcaag gtgcgggcgg cggccaccac gctgctcggg ctgagccgca
      421 cgatcggacg atccaaagcg gcccgcagca cggacctgca gcaggccgcc cagtccgaac
      481 cgcagttgcc gcacctcacc ctggtacccg tggacgaggt actcacgccc gtctcggcgg
      541 ccaaggagca ccccgtcgac gcggcgcagg agcggctcac cctgcttcga gacgcgatga
      601 atgccgagga tctggccacg gcgttcgtgg tcaccgcccg ggtgctggac gaggtgcacg
      661 cgctgcccgc cgatcacgtg cgcgaaggcc tggccgaact cgaagacgtg ctgatcgagg
      721 tggcgcggca ctgcgctccg cgactggtgc cgcacctgtc gggcatggtg cagcgccagc
      781 gcgaccgccg caccagttca cccaaccgct acaagtccca gctgtacgcc gagcgcatcc
      841 tcggcatggt gctggacgag atcatccggt gccgtccgca tcgtccacgc gatctgcgcg
      901 acctgctgaa cgacatcgcg cggcgtccga accgggccct gtcgctcacc gaggccgcgg
      961 cgttcaccca ctggtcgccc ggttacctgt cgaagatgtt caaatcggtc acgggctgca
     1021 ctttcgtgtc gtatctgatg cagtggcgta tctcgcgggc gaagctgatg ctagcttcca
     1081 cccagatgcc ggtacgcaag atcgcggcgg agctcgactt caaccaggtc aactacttct
     1141 ccagagtctt ccgggcgcac acgggacaca cgcccagcga gtaccggcgt tcgacctcgc
     1201 gggaggaacg accgttcgat gcatcgcttt cagctcacca ccacactgtg ttacgggcct
     1261 gacgcactgt cggccctggg cgccctggcc gaacgccggg tgctgctggt cacggacgcc
     1321 ttcctggcgg ccggtgacct gatggcattg gtgcgccgcg agctgcggga tgccgaggtg
     1381 aaggtgttcg acgcggtgcg gcccaacccc gacatcgccg ccgtgaccga ggggctgcgc
     1441 agttttctgg ccttctcgcc ggacgcgatc gtcgcgctcg gcggtggttc gccgatcgac
     1501 accgcgaagg cggtgcgcaa gctggcgctg gagcagggtc acgatctacc ggcgggtctg
     1561 gtggtgatgc cgaccaccag cgggtccggc tccgaggtca ccgcgttcgc tgtcgtcacc
     1621 gacgccgaga gtcacgccaa actgccactg acttcggtcg acatgctgcc tgaggtcgcc
     1681 atactggatg ccgaggcggt gctcaccgca ccccctcgcc tcaccgccga ctcgggcatg
     1741 gacgcggtca gccacgcgat cgaggcctac gtggcgaagg gtgccaacgt gttcagcgac
     1801 gcgctggccg agaaggcgct gcgactggcc gatgccgcgc tcgtgcggtg ctttgtggac
     1861 ggcagcgatg cgcaggctcg cgaacagcag cacgcggccg cgacgatggc cggtatcgcc
     1921 ttccagaacg ccggactggg catcgtccac ggtctttcgc acgccatcgg cggttcgttc
     1981 aaggtggccc acggacgcct caacggcatc ctgctgccac acgtcatcgc cttcaacgca
     2041 ggcccgctcg ggctgcgtcc ccacgagctg tcggacgtcg cccgccgcta cgccgcgctg
     2101 gccagggcga tggggctgca ggcctccaat gagcgcaacc tggtgctggc cctgagcggc
     2161 tgggtggagc ggttgcgccg cgacctcgcg atgcccgcca cggtgacggc ggcaggggtg
     2221 ggacgcgccg agttcctggc ggcggttccg gcattggcgg tgaccgcgct gaccgacttc
     2281 tgcacgtccg gcaacccggt cgacgtcagc gaagccgacc tgaccgggat cctgcgcgcc
     2341 gtggtgtgag cggttcagcg cagccgcgcc tccagcgcct cgattccttc acccgtgata
     2401 gccgatacct cgaagacctc cagcgctccg gccaggcgta ggtagtcgcc ggccaggctc
     2461 acctggcggg gctcggcgat gtcgaccttg gtcaccacac cgatcgaagg gggcatgaag
     2521 aacgtggtga aaccgggggg gaacttcgac tcgttgacgg tggccgatgc cagcagcacg
     2581 accagctcgg catcgaagct cgccatgcgc agcgagtgct tgaaccaggg caactcgagg
     2641 tactcaccgg gtgtgtcgat ggtctggccg tcggtgtaga tggtctgcgt cttggagtag
     2701 ctgatctcgt ccccgtgcag ccgttgcaga agagtggtct tgccacaccc cacgctgccg
     2761 acgagcagga tcttcttcac gtgctggtca gcggcggacg cgcgaagccc aggttgtcgc
     2821 acagcccgtc gaccacgctg tgcaccgccg tctgcacgtc gacgagacgt ccgatcacca
     2881 ccagcgagcc agagaaccga tcgacgaagc cgagttcgac atcggccgac ttggtggcga
     2941 tgtcggccgc gattatcgcg gcttcgctag gcgtgatggt catcacgccg atggcgccgc
     3001 cctgcggcgg cagcccgatc ttggggaaca gcttctcgtc ggggttggcc accacgtggg
     3061 ccaacgtgac ctgcttgccc ggaacgaact cctggatgat gcgctgcttg tcgtcggcca
     3121 cgtcacctcc tcgtgcgaac cggcacggac gacacccgtg cccacccatc gtccggacgc
     3181 cggcgtcgcg gtgtcaaccg ccaccgggta actggacgcc cccgtgctga ggtgcaccta
     3241 ttggctctgc cagttgcact ttctggctct gtcgaggccg gccagcaggc cggaaagtcg
     3301 tccccgcgca gccgtttagt gccgtgaccc ccggcctaac ctctgatcga atgcccgccg
     3361 gatcatccag acctgcggcc caacccaacg aaggagttcg gggatgcaag aagccctcgg
     3421 aatggtggag acgaaaggtt tcgtcgccgc agtggaagcc gccgacgcga tggtgaagtc
     3481 ggccaacgtc gtgctgatcg gaacccagcg ggtgggctcc ggcctggtca ccgtgctggt
     3541 ccgtggtgat gtcggcgccg tcaaggccgc caccgacgcc ggcgccgtcg ccgcccagaa
     3601 cgtgggcgag ctcgtctccg tgcacgtcat tcctcgcccg cacaccgacg tggagaagat
     3661 cctcccgtcc accgagaagt gaggctgacc caccatggct agtgaagacc tcgtcaacca
     3721 gatcatggcc gcggtcatga agaagatcga ctccccggct gctgcagcgg cacccacgaa
     3781 gaccgcgtcc ggcgcccgtc cggccagcac cgagttcgtc ggttgcagcc cgatcggcga
     3841 caccatcggc ttggtcatcg ccaacgtcga cccgcagatc cacgacctga tgaagatcga
     3901 cggcaagtac cgctcgatcg gcatcgtcgg tgcccgcacc ggcgccggcc cgcacatctt
     3961 cgccgcggac gaggcggtca aggccaccaa caccgaggtg ctcaccatcg aactgccccg
     4021 cgacaccaag ggcggcgccg gtcacggttc gttgatcatc ttcggagccg aggacgtcag
     4081 cgacgcccgt cgcgccgtcg aggtcaccct gagcgagatc gagcgcacct tcggcgacgt
     4141 gtacgccaac gacgccggtc acctggagtt ccagtacacc gcccgcgcca gcctcgccct
     4201 gaacaaggcg ttcggcgctc cgctcggcaa ggccttcggc atcaccgtcg gcgctcccgc
     4261 cgcgatcggt gtggtgctgg ccgacacggc cgtcaaggcg tccaccatcg acgtgatcgg
     4321 ctactccagc ccggccgccg gcaccagctt cagcaatgag gtgatctcgt tcttcagcgg
     4381 tgattccgga gcggtccgcc aggccatcat cgctgcccgt gaggtcggcc gcggtctgct
     4441 cggcgccatg ggcgactacc ccgaatccgc caccaccccg tacatctgag aacgagggaa
     4501 tccgtgaaga gcaagcgatt cgaggcgctc gacgaccgtc cggtcaacaa ggacggcttc
     4561 gtcaccgagt ggccagaagt cgggctgatc gccatgaacg gcccgaacga cccccaaccg
     4621 tcgatccgga tccagaacgg caaggtcgtc gagctcgacg gcaagagccg cgacgagttc
     4681 gacatgatcg acaccttcat cgccgactac ggcatcaagc tcgacaacgc cgaggtcgtc
     4741 aacgcgatgg actcgctcga gctggcccgc aagctggtcg acatcaacgt gccgcgcgcc
     4801 gaggtggtca agctgaccac cgccatgacc cccgcgaaga tctgcgaggt gatcggcaaa
     4861 atgacggtgc tcgagatgat gatgtcggtc accaagatgc gggcccgcaa gtcgccggcg
     4921 aaccaggccc atgtgaccaa cctcaaggac aacccggtac agatcgccgc cgatgcggcc
     4981 gaggcctcgc tccgtggctt cgccgagttg gagaccacgg tcggcatcgc ccgctacgca
     5041 ccgttcaacg cgatcgccct gatggtcggc gcgcaggtcg gacgccccgg catcctcacc
     5101 cagtgcgcgg tcgaagaggc caccgaactg aacctgggca tgcgcggctt caccgcgtac
     5161 gccgagacgg tgtcggtcta cggcaccgag ccggtgttca tggacggcga cgacaccccg
     5221 tggtcgaagg ccttcctggc ctcctgctac gcctcgcgcg gcctgaagat gcggttcacc
     5281 tcgggcaccg gttccgaggt gcagatgggc tacgccgagg gcaagtcgat gctgtacctg
     5341 gaggcccgct gcctctacat caccaaggcc gccggctcac agggcatcca gaacggctcc
     5401 gtgtcgtgcg tgggtgttcc cgccgccgtg cccggtggta tccgggcgat cctggccgag
     5461 aacctgatcg cgaccgcgct cgacctggag tgcgcctcgt ccaacgacca gaccttcacc
     5521 cactccgacc tgcgccgggt cgcccgctcg ctgatgcagt tcgtgcccgg cacggacttc
     5581 atctgctcgg gctactcggc cacgccgaac tacgacaaca tgttcgccgg ctcgaactgg
     5641 gacgccgacg actacgacga ctggatcatc attcagcgtg acctcaaggt cgacggcggt
     5701 ctggtgccgg tgctcgaaga ggacgtcgtg cgggtgcgca acaaggccgc caaggtgatt
     5761 caggcggtgt tccgcgaact cggtctgacc gaggtcaccg acgaagaggt cgaggccgcc
     5821 acctacgccc gtggcagcca ggacatgccc gaccgcaacg tggtcgaaga cctcaaggcc
     5881 gcagaagacc tgctcaagcg cggactgacc ggtgtcgaca tcgtgaaggc gctggccaag
     5941 tccggtttcg aggacgttgc atcgttcgtc tacggcatgc tgcggcagcg cgtcgcgggt
     6001 gactacctgc acacgtccgc gattctcaac gacaagttcg aggtgatcag cgcggtcaac
     6061 cagcccaacg actacaacgg gccgcagacc ggctaccaga tctcggacga gaagtgggag
     6121 ctgttgaaga ccatccgcca ggcgatcagc cctgagaacg tctgaggaga ctgagatgga
     6181 cgaaaaaaca ctgcgcagca tcatcgaaga ggtcatgaag gagttcggcg caggctccgg
     6241 cggcggtacc gcaaccgccg tcatgacggc accgagcgtc agctcggcgt ccggaaccct
     6301 gagcatcagc gaggccggcc cggccgaccg cggcaccaac ccccgtgagg tggtgatcgc
     6361 gctggctccg ggcttcggtg gccggatgac cagcacgatc accggcatcg atcacggcga
     6421 ggtgctgcgt gaggtgtgcg cgggcatcga ggagcagggg ctgacctacc ggttcatccg
     6481 ggtgttccgc accgccgacg tcggcttcat cgcccatgag gccgcgaagc tctcgggctc
     6541 gggcatcggc atcggcatcc agtcccgcgg cacgacggtg atccaccaga aggacctgcc
     6601 gccgctgtcg aacctggagc tgttcagcca ggccccgctg atcgacctcg acacgttccg
     6661 ggcgatcggc gagaacgccg ccaagtacgc caagggcgag tcgccgtcgc cggtaccggt
     6721 ccgcaacgac cagatggccc ggccccgcta tcaggccatc gcggccctgt ggcacatcaa
     6781 ggagacgcag ctctgcgatc cccacaagaa gacgatgacc ctgcgggtcg agtaccggta
     6841 gaggagcgac atggaatccg aagaactgat ccgtcagatc atggacgagg tgatgaagaa
     6901 cctcgccaag gaccaggtgt cgttcgcgaa gaagccggcg gccaatggct cggcaggctc
     6961 ggtcagtgcg gccaactacc cgctgggcga gaagatgccc gagaagatca agtcggccac
     7021 cggcaaggtg ctctcggact tcagcgccga gaaggtgatc tcaggccagc tcaccgccga
     7081 ggacttccgg atcgccccgg agaccctcga gttgcaggcc caggtcgccg agtccgtcgg
     7141 acgcgacacc ctggcccgca acatgcggcg ggcggccgag ctgatcaagg tgccggacga
     7201 cgagttgctg caggtctaca actcgctgcg tccgtaccgc tcgacgaagg ccgagctgta
     7261 cggcatcgcg gacgggctgg agagcaagta cggctgcacg gtgaacgccg cgttcatccg
     7321 tgaggccgcc gacgtgtacg agaagcgcgg acggctcaag gccgactgac cgggacgccg
     7381 ggatgacgaa atccccc
//
LOCUS       DAOSUG010000086         7368 bp    DNA     linear   ENV 10-JUN-2024
DEFINITION  MAG TPA_asm: Micropruina sp. isolate AMR_MDS_2195
            NODE_4999_length_7368_cov_4.328593, whole genome shotgun sequence.
ACCESSION   DAOSUG010000086 DAOSUG010000000
VERSION     DAOSUG010000086.1
DBLINK      BioProject: PRJNA925477
            BioSample: SAMN32864262
            Sequence Read Archive: SRR4236673
KEYWORDS    WGS; ENV; Metagenome Assembled Genome; MAG; Third Party Data; TPA;
            TPA:assembly.
SOURCE      Micropruina sp. (wastewater metagenome)
  ORGANISM  Micropruina sp.
            Bacteria; Bacillati; Actinomycetota; Actinomycetes;
            Propionibacteriales; Nocardioidaceae; Micropruina.
REFERENCE   1  (bases 1 to 7368)
  AUTHORS   Abdulkadir,N., Saraiva,J.P., Zhang,J., Stolte,S., Gillor,O.,
            Harms,H. and Rocha,U.
  TITLE     Genome-centric analyses of 165 metagenomes show that mobile genetic
            elements are crucial for the transmission of antimicrobial
            resistance genes to pathogens in activated sludge and wastewater
  JOURNAL   Microbiol Spectr 12 (3), e0291823 (2024)
   PUBMED   38289113
REFERENCE   2  (bases 1 to 7368)
  AUTHORS   Abdulkadir,N.
  TITLE     Direct Submission
  JOURNAL   Submitted (24-MAR-2023) Environmental Microbiology, Helmholtz
            Center for Environmental Research -UFZ, Permoserstr 15, Leipzig
            04318, Germany
COMMENT     The annotation was added by the NCBI Prokaryotic Genome Annotation
            Pipeline (PGAP). Information about PGAP can be found here:
            https://www.ncbi.nlm.nih.gov/genome/annotation_prok/
            
            ##Genome-Assembly-Data-START##
            Assembly Method        :: SPAdes v. 3.11.1
            Genome Representation  :: Full
            Expected Final Version :: Yes
            Genome Coverage        :: Not Applicable
            Sequencing Technology  :: Illumina MiSeq
            ##Genome-Assembly-Data-END##
            
            ##Genome-Annotation-Data-START##
            Annotation Provider               :: NCBI
            Annotation Date                   :: 05/04/2023 04:57:10
            Annotation Pipeline               :: NCBI Prokaryotic Genome
                                                 Annotation Pipeline (PGAP)
            Annotation Method                 :: Best-placed reference protein
                                                 set; GeneMarkS-2+
            Annotation Software revision      :: 6.5
            Features Annotated                :: Gene; CDS; rRNA; tRNA; ncRNA
            Genes (total)                     :: 2,776
            CDSs (total)                      :: 2,750
            Genes (coding)                    :: 2,714
            CDSs (with protein)               :: 2,714
            Genes (RNA)                       :: 26
            tRNAs                             :: 24
            ncRNAs                            :: 2
            Pseudo Genes (total)              :: 36
            CDSs (without protein)            :: 36
            Pseudo Genes (ambiguous residues) :: 0 of 36
            Pseudo Genes (frameshifted)       :: 2 of 36
            Pseudo Genes (incomplete)         :: 32 of 36
            Pseudo Genes (internal stop)      :: 2 of 36
            ##Genome-Annotation-Data-END##
FEATURES             Location/Qualifiers
     source          1..7368
                     /organism="Micropruina sp."
                     /mol_type="genomic DNA"
                     /submitter_seqid="NODE_4999_length_7368_cov_4.328593"
                     /isolate="AMR_MDS_2195"
                     /isolation_source="Activated sludge of municipal
                     wastewater treatment plants"
                     /db_xref="taxon:2737536"
                     /environmental_sample
                     /geo_loc_name="Canada"
                     /lat_lon="49.25 N 123.1 W"
                     /collection_date="2015-06-16"
                     /metagenome_source="wastewater metagenome"
                     /note="metagenomic"
     gene            <1..197
                     /locus_tag="PLF56_04225"
     CDS             <1..197
                     /locus_tag="PLF56_04225"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_002529963.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=3
                     /transl_table=11
                     /product="Asp-tRNA(Asn)/Glu-tRNA(Gln) amidotransferase
                     GatCAB subunit B"
                     /protein_id="HOQ52800.1"
                     /translation="AVVSDDGALTEAVDAAIAANPEVAQRIRDGKVQAAGALIGQVMK
                     AMKGQADAGRVRELILQQLN"
     gene            complement(341..946)
                     /locus_tag="PLF56_04230"
     CDS             complement(341..946)
                     /locus_tag="PLF56_04230"
                     /inference="COORDINATES: ab initio
                     prediction:GeneMarkS-2+"
                     /note="Derived by automated computational analysis using
                     gene prediction method: GeneMarkS-2+."
                     /codon_start=1
                     /transl_table=11
                     /product="hypothetical protein"
                     /protein_id="HOQ52801.1"
                     /translation="MEPVRTLFSATPRPAVASRHGHSWEASDYDLLVEGIRQGLDVPA
                     LAERLGRVERTVYPRVRRLLPLAQRDCLSELVLSAARTALQDTEYDWRSEMLLTPAPA
                     PIVRNEIVHTGLAGLTNEQLVPIGYALMAVSGELQEEPLAGVVRELDERGLQPDLIEL
                     RAHLSVRASPQPISEGAAWASAMYWVTGRSNVTRWRNQAEW"
     gene            complement(1180..1608)
                     /locus_tag="PLF56_04235"
     CDS             complement(1180..1608)
                     /locus_tag="PLF56_04235"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_017934114.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="VOC family protein"
                     /protein_id="HOQ52802.1"
                     /translation="MDARVNFITLAVDDLALARRFYVDGLGWPPALDGSEVVMIRLSP
                     TLVLSLWSADEFEAEVGPIRRGDGIVPFTLAHNVPNPDAVDRVLADAQAAGAASVSPG
                     RTRDWGGYSGYFADPDGVRWEVAYNPGDLGRELMATEGLG"
     gene            complement(1707..2897)
                     /locus_tag="PLF56_04240"
     CDS             complement(1707..2897)
                     /locus_tag="PLF56_04240"
                     /inference="COORDINATES: protein motif:HMM:NF012607.2"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="phosphoglycerate dehydrogenase"
                     /protein_id="HOQ52803.1"
                     /translation="MGDAHITMPFRIRTLNAISQVGLTRLPADRYTVGHDVSSPDALL
                     VRSASLHNLPIEPSVLAVARAGAGTNNIPIAEFSKVGVPVFNTPGANANAVKELVVAG
                     LFLAARNIVDAAAFAHRLEGDHATMDKLVEAGKKNYVGFELPGRTLGVIGLGAIGVEV
                     ANVALSLGMNVLGYDPGITVEHAWALSSQVQHVTDLNELLKRSDIVTLHVPLVAGTKG
                     LISTDAFAAMKSSAVLLNFARGPIVDEAALVAALEGGQIRGYVCDFPTPTLNKRPRVV
                     TLPHLGASTNEAEENCARMAADELRWYLEDGTIQNSVNFPDARLPRAEGSSRLAIANS
                     NVPNMVGQISTMLAEAGLNISDLLNKSRGELAYTLVDVDGAVPDDLLQRIQGIEGVLK
                     ARLL"
     gene            complement(2890..3969)
                     /gene="serC"
                     /locus_tag="PLF56_04245"
     CDS             complement(2890..3969)
                     /gene="serC"
                     /locus_tag="PLF56_04245"
                     /EC_number="2.6.1.52"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_016668607.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_function: GO:0003824 - catalytic activity [Evidence
                     IEA];
                     GO_function: GO:0004648 -
                     O-phospho-L-serine:2-oxoglutarate aminotransferase
                     activity [Evidence IEA];
                     GO_process: GO:0006564 - L-serine biosynthetic process
                     [Evidence IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="3-phosphoserine/phosphohydroxythreonine
                     transaminase"
                     /protein_id="HOQ52804.1"
                     /translation="MRVYNFSAGPAMLPEPVLAQAQAELLDWNGSGMSVMEVSHRSKE
                     FVACAAQAEATLRRIMGIPDNYKVLFLQGGAYGQFAAVPLNLAPDGAPVDFLLTGDWS
                     KKAVAEAKKYVTPRVIADESAGNFTKAPAQGSFEVGADAAYLHYTPNETIRGVEFGYV
                     PETGDVPLVADLSSTILSRPIEVSKFGVIFAGAQKNMGPSGMCVVIVRDDLLERARAT
                     TPGVWNYAQMAAADSMLNTPPTFGWYLLGLVYQWVADQGGLAAMGERNRAKAEALYGF
                     IDGSDFYRTPVQADSRSWMNVPFLTAKPELDKSFVSEAAAAGLTNLGGHRSVGGMRAS
                     IYNAMPIEGIQALIDFMKEFERANG"
     gene            4126..4557
                     /locus_tag="PLF56_04250"
     CDS             4126..4557
                     /locus_tag="PLF56_04250"
                     /inference="COORDINATES: ab initio
                     prediction:GeneMarkS-2+"
                     /note="Derived by automated computational analysis using
                     gene prediction method: GeneMarkS-2+."
                     /codon_start=1
                     /transl_table=11
                     /product="hypothetical protein"
                     /protein_id="HOQ52805.1"
                     /translation="MHPLDALQRPGSLTRRLLWALLALALAAHLYGLYSPGEPGAVEL
                     FPQADKVFHLLGFAVPSMLAVLLTRHWWPIAVLAGNAVLSEIVQHFWLPGRDGDLADV
                     AADLAGLLPAVAVYYWFAARSSSTASSSPTKRTSAAARPKA"
     gene            complement(4473..5099)
                     /locus_tag="PLF56_04255"
     CDS             complement(4473..5099)
                     /locus_tag="PLF56_04255"
                     /inference="COORDINATES: protein motif:HMM:TIGR03085.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_function: GO:0046872 - metal ion binding [Evidence
                     IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="TIGR03085 family metal-binding protein"
                     /protein_id="HOQ52806.1"
                     /translation="MSHAQTERAFTCDALLTVGPSAPTLCEGWTAHDLAAHLWLRENE
                     QLSALGFFVPALQQRTDDRMAQLKGQLSYDELVDLIRQGPPRFSVFGLPGADEAANSV
                     EYLIHGMDLRRPNDLPEPERDAEFLDWCWSRTSKVAPLMLRKSPVALVLEWDGRRDHS
                     VRVGKGDRIVTVIGRPDELLLYAFGRAAAEVRFVGLDEAVDELRAANQ"
     gene            5146..5526
                     /gene="hisI"
                     /locus_tag="PLF56_04260"
     CDS             5146..5526
                     /gene="hisI"
                     /locus_tag="PLF56_04260"
                     /EC_number="3.5.4.19"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_015070537.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_function: GO:0004635 - phosphoribosyl-AMP
                     cyclohydrolase activity [Evidence IEA];
                     GO_process: GO:0000105 - histidine biosynthetic process
                     [Evidence IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="phosphoribosyl-AMP cyclohydrolase"
                     /protein_id="HOQ52807.1"
                     /translation="MSLDPSIAALLKRNPDHLVPAVVQDASSRQVLMLAWMDDEALAR
                     TLATRKGTYFSRSRQQYWVKGETSGHTQQVREVRLDCDGDTLLVLVDQTGPACHTGAT
                     SCFTDRVLLASAEVDATTQERADA"
     gene            5519..7030
                     /locus_tag="PLF56_04265"
     CDS             5519..7030
                     /locus_tag="PLF56_04265"
                     /EC_number="4.1.3.27"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_014846702.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_function: GO:0004049 - anthranilate synthase activity
                     [Evidence IEA];
                     GO_process: GO:0000162 - tryptophan biosynthetic process
                     [Evidence IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="anthranilate synthase component I"
                     /protein_id="HOQ52808.1"
                     /translation="MPELVLTPDLPEFLPLAATRRVVSVHARLLADDLTAVGLYHQLC
                     GRRDNTFLLESADNGMWSRWSFVGVNAVATLSEHDGHAVWSGREIVGLPTGGDPLRVL
                     AETLELLHTPADDALPPLVSGMVGYLAYDVVRRLEVLPDDNPDDLQLPELVMMLVSDM
                     AILDHHTGEVWLVANAINFDDSPERAEQAWSDARDRVLAMAARLAEPAEPLVAVTDEA
                     VPAPAIVRQQTPEQYMAAVSAAVEEIKAGEAFQIVPSQRFDLTTDADPLDVYRLLRRS
                     NPSPYLYLLRLPGLAVVGSSPEALVTVQNGRAETHPIAGSRPRGRSAADDAAMEAQLF
                     ADPKERAEHLMLVDLGRNDLGRVCAPGTVEVVEFMKVRRYSHIMHLEAAVVGELSPGR
                     SALDVTLSCFPAGTLSGAPKVRAMQIIDGLEVSRRGLYGGGVGYFDFAGNSDVAIAIR
                     TAVLRNGVAHVQAGAGIVADSVPASEEQEVQHKAAAVLRAIEGAHRLRRLNGA"
     gene            7044..>7368
                     /locus_tag="PLF56_04270"
     CDS             7044..>7368
                     /locus_tag="PLF56_04270"
                     /inference="COORDINATES: ab initio
                     prediction:GeneMarkS-2+"
                     /note="Derived by automated computational analysis using
                     gene prediction method: GeneMarkS-2+."
                     /codon_start=1
                     /transl_table=11
                     /product="hypothetical protein"
                     /protein_id="HOQ52809.1"
                     /translation="MSLAMLWGGAGLALVASGQPWWSAGSSSETGSGATSGAASVLVL
                     AAAAGAFLSSWLRRRAGRLVSCLVALLLVGAAVVAATAGAPVSLPGSALTNAAVTATA
                     WRWVYL"
BASE COUNT         1135 a         2544 c         2562 g         1127 t
ORIGIN      
        1 tcgcggtggt gtcggacgac ggtgccctga ccgaggcggt ggacgccgcc atcgcggcca
       61 atcccgaggt ggcgcagcgc atccgcgacg gcaaggtgca ggccgcgggc gcactgatcg
      121 gccaggtgat gaaggcgatg aagggccagg cggacgcggg tcgcgtccgg gagttgatcc
      181 tgcagcagct gaactgaccg ctggtcggga cttccgctgg acgagccggt cgggacctac
      241 cgctggacga gccggtcgat acctaccgcg gatcggcgca tcgagaccta tgtccgccat
      301 tctgggtggg ccgcccggct cacggcgtgc aggccactcg tcaccattcg gcctggttgc
      361 gccatcgagt cacgttgctg cggccggtga cccagtacat cgcgctggcc cacgcggcgc
      421 cctcggagat cggttgcggc gaggcgcgaa ccgacaggtg ggcacgaagc tcgatcaggt
      481 ccggttgaag accacgctcg tccagctcgc ggacgacccc ggcgagcggc tcctcttgca
      541 actcaccgga aacggccatc agtgcgtacc cgatcggcac cagttgctcg ttggtgaggc
      601 cggccaggcc ggtgtgcacg atctcgttgc gcacgatcgg tgccggggca ggggtgagca
      661 gcatttcgga gcgccagtcg tactcggtgt cttgcagggc cgtgcgtgcg gcgctcaaca
      721 ccagttcgga caggcaatcg cgctgggcca acggcagcag ccgccgcacc cgcgggtaca
      781 ccgttcgctc cacccggccg agacgctcgg ccagggcggg cacgtccagc ccttgccgga
      841 tgccctcgac gagcaagtcg taatcggacg cctcccacga gtgaccgtgc cggctcgcga
      901 cggccggacg gggagtggcg ctgaacaggg ttctgacggg ttccacggat cacctttcgt
      961 tcgatgtggc gatcctggcc gtgaggtgtt gtcacaggtg tggacgcaag gtgcaccggt
     1021 gttgtccgca cgatgtgcga acgtccagat ctcaaccgct tcgacggtgg taggcgagca
     1081 tggccacgcg taggccacca ccaggatcgc cacgcaccat gccaccgcga tcccgagttg
     1141 tgcgccgacc ggctacaggt cgaaccggag gtagcggggt cagcccaaac cctcagtggc
     1201 catcaactcc cggccgaggt cgcccgggtt gtacgccacc tcccagcgga cgccgtccgg
     1261 gtcggcgaag tagccgctgt agccgcccca gtcgcgcgtc cggcccggac tgacggacgc
     1321 ggcgccggca gcctgcgcgt cggccagcac ccggtcgacc gcgtcggggt tgggtacgtt
     1381 gtgcgccagg gtgaacggca cgatgccgtc accgcgccgg atcgggccga cttcggcctc
     1441 gaactcgtcc gcgctccaca acgacagcac caacgtgggg gagagcctga tcatcaccac
     1501 ctcggaccca tccagcgccg gcggccagcc cagcccgtcc acgtagaagc gccgggccag
     1561 cgccaggtcg tcgacggcga gggtgatgaa gttgacccgt gcgtccatga tccgaacctt
     1621 agaaccccag cgaaccccca cctaaccatc acccgcctgc gaagcttcgc aggcgggtga
     1681 tagttaggtg ggggttcggc cgagggtcag agcagccggg ccttcagcac accctcgatg
     1741 ccctggatgc gctgcagcag gtcgtccggc accgcaccgt ccacatcgac cagcgtgtag
     1801 gccagctcac cacgcgactt gttcagcagg tccgagatgt tcaaccccgc ctcggccagc
     1861 atggtcgaga tctggcccac catgttcggc acgttgctgt tggcgatcgc cagccggctg
     1921 ctgccctcgg cgcgcggcaa ccgggcgtcc ggaaagttca ccgagttctg aatcgtgccg
     1981 tcctcgaggt accagcgcag ctcgtcggcg gccatccggg cacagttctc ttcagcctca
     2041 ttggtggacg ctcccagatg cggcagagtg accacccggg gccgcttgtt cagcgtcggg
     2101 gtgggaaagt cgcagacata cccacggatc tgaccgccct cgagcgcggc aaccagcgcg
     2161 gcttcgtcca cgatgggtcc gcgggcgaag ttgagcagca cggcggagct cttcatggcg
     2221 gcgaacgcgt cggtgctgat caggcccttg gtgcccgcga ccagcggcac gtgcagggtg
     2281 acgatgtcgg agcgcttgag caactcgttc aggtcggtga cgtgctgcac ctgcgacgac
     2341 agcgcccagg cgtgctcgac ggtgatgccg gggtcgtagc cgagcacgtt catgcccagc
     2401 gacaacgcca cgttggccac ctcgacgccg atcgccccga ggccgatcac acccagggtg
     2461 cgaccgggca gttcgaagcc gacgtagttc ttcttgcccg cctcgaccag cttgtccatg
     2521 gtggcgtggt caccctcgag ccggtgggcg aaggcggcag catccacaat gttgcgggcg
     2581 gccaggaaca gaccggccac caccagctcc ttcaccgcgt tggcgttggc gccgggggtg
     2641 ttgaacaccg gcacgcccac cttgctgaac tcggcgatcg gaatgttgtt ggtgcctgcc
     2701 ccggcccgcg ccaccgccag cacggacggc tcgatgggca agttgtgcag gctcgcggag
     2761 cgcaccaaca gggcgtccgg agagctcacg tcgtgaccga cggtgtagcg gtcggcgggc
     2821 aggcgcgtaa gcccgacctg gctgatggcg ttcagggtac gaatgcgaaa gggcatggtg
     2881 atgtgagcgt cacccattgg cgcgctcgaa ctccttcatg aagtcgatca gggcctggat
     2941 gccctcgatg ggcatcgcgt tgtagatgct agcccgcatg ccacccacac tgcggtgccc
     3001 gcccaggttc gtgagaccgg ccgcagcggc ctcgctgacg aaggacttgt cgagttcggg
     3061 cttggcggtc aggaacggca cgttcatcca cgagcggctg tcggcctgga cgggggtgcg
     3121 gtagaagtct gagccgtcga tgaagccgta gagcgcctcg gccttggccc ggttgcgctc
     3181 gcccatggcg gccagtccgc cctggtcggc gacccactgg tagaccaggc ccagcaggta
     3241 ccagccgaag gtcggcgggg tgttcagcat cgagtcggcc gccgccatct gagcgtagtt
     3301 ccacaccccc ggcgtggtcg cacgggcgcg ctcaagcagg tcgtcgcgca cgatcaccac
     3361 gcacatgccc gaggggccca tgtttttttg ggcgccggcg aagatcacgc cgaacttcga
     3421 cacctcaatg ggacgcgaca ggatcgtcga gctcaggtcg gcgaccagcg gcacgtcgcc
     3481 ggtctcgggc acgtagccga actcgacgcc gcggatcgtc tcgttgggtg tgtagtgcag
     3541 gtaggcggca tcggcgccga cctcgaacga tccctgcgcc ggagccttgg tgaagttgcc
     3601 ggccgactcg tcggcgatca cccggggggt cacgtacttc ttggcctcgg cgacggcctt
     3661 cttcgaccag tcgccggtga gtagaaagtc gaccggagca ccgtccgggg caaggttgag
     3721 cggcacagcg gcgaactggc cgtaggcgcc gccctgaaga aagagcacct tgtagttgtc
     3781 gggaatgccc atgatccgac gcagcgtggc ctcggcctgc gccgcacagg cgacgaactc
     3841 cttgctgcgg tgcgacacct ccatcaccga catgccggag ccgttccagt cgagcagttc
     3901 ggcctgggcc tgggccagga cgggctcggg aagcatggcg gggccggctg agaagttgta
     3961 gacgcgcatg tgctgattct ctcagcgttt gccgggtcgg cgcagcagcc gccgccattc
     4021 gggccgagtc gccgaacgcc cccctaagta tctgccgccc gcgaagcttc gcgggcggca
     4081 gatacttagg ggggcgctgg gtcggccggc tggcagactg gcggcgtgca cccgctcgat
     4141 gccctgcaac ggcccggcag cctgacccgg cggctgctgt gggcgctgct ggcgctggcg
     4201 ctcgcggcgc acctgtacgg gctctactcc ccgggcgagc ccggtgcggt ggagttgttc
     4261 ccgcaggccg acaaggtctt tcaccttctg gggttcgcgg tgccctcgat gctggcggtg
     4321 ctgctcaccc ggcactggtg gccgattgcc gtgctggccg gtaacgcagt gctgagcgag
     4381 atcgtccagc acttttggct gcccggacgc gacggcgacc tggccgacgt ggccgccgac
     4441 ctggccgggt tgctgcccgc cgtcgctgtc tactactggt tcgcggcccg cagttcgtcg
     4501 accgcctcgt cgagccccac gaagcgcacc tcggccgccg cccgtccgaa ggcgtagagc
     4561 agcaactcgt ccggacgacc gatcacggtc acgatgcggt cgcccttgcc gacccggacg
     4621 gagtggtccc ggcgcccgtc ccactccagc accagcgcca ccggggactt gcgcagcatc
     4681 agcggcgcga ccttggaggt gcgcgaccaa caccagtcca ggaactcggc atcgcgctcg
     4741 ggctcgggca gatcgttggg gcggcgcagg tccatgccgt ggattaggta ctccaccgag
     4801 ttggcggcct catcggcacc cggcagcccg aacaccgaga accgcggcgg cccctggcgg
     4861 atcaggtcga ccagttcgtc gtagctcaac tgccccttga gttgcgccat gcgatcgtcc
     4921 gtacgctgct gcaacgccgg cacgaagaag ccgagcgcgc tgagttgttc gttctcacgc
     4981 agccacaggt gcgccgccag gtcgtgcgcc gtccagccct cgcacagggt cggggcggac
     5041 gggccgacgg tcagcagggc atcacaggtg aaggctcgtt cggtctgggc atgcgacatg
     5101 cggccagcgt aggggaccgg ccaacagccc tgacacaatg accgggtgag tctcgaccct
     5161 tccatcgccg cactgctcaa gcgcaacccc gaccacctgg tgcccgccgt ggtgcaggac
     5221 gccagttcgc gccaggtgct gatgctggcc tggatggacg acgaggcgct ggcccgcacc
     5281 ctcgccactc gcaagggcac ctacttctcg cgcagccgac agcagtactg ggtcaagggc
     5341 gagacctcgg gtcacaccca acaggtgcgt gaggtgcgcc tcgactgcga cggcgacacg
     5401 ttgctggtgc tcgtcgacca gaccgggccc gcctgtcaca ccggggccac cagctgcttc
     5461 accgaccggg tgctgctggc ctccgccgag gtggacgcga ccacccagga gcgcgccgat
     5521 gcctgagttg gtcctcaccc ccgacctgcc tgagtttctg ccactcgccg ccacccggcg
     5581 tgtggtcagc gtgcacgcca ggctgctcgc ggacgacctg accgccgtcg ggctgtacca
     5641 ccagctctgc ggacggcgcg acaacacctt cctgctcgaa tccgccgaca acggcatgtg
     5701 gtcgcgctgg tcgttcgtcg gcgtcaacgc ggtggcaacg ctgagcgagc acgacggaca
     5761 cgccgtctgg agcggacgcg agatcgtcgg gctgccgacc ggcggtgacc cgctgcgggt
     5821 gctggccgag acgctcgagc tgctgcacac gcccgcggac gacgcccttc cgcccctggt
     5881 ctcgggcatg gtcggctacc tcgcctacga cgtcgtccgg cgcctcgagg tgctgcccga
     5941 cgacaacccc gacgacctgc agctgcccga actggtgatg atgctcgtca gcgacatggc
     6001 gatcctcgac caccacaccg gcgaggtctg gctggtcgcc aacgcgatca acttcgacga
     6061 ctcacccgag cgggccgagc aggcctggag cgacgcccgc gaccgggtgc tggcgatggc
     6121 ggcccggctg gccgaaccgg ccgaacccct ggtcgcggtc acggacgaag cggtgcccgc
     6181 gccggccatc gtccggcagc agacacccga gcagtacatg gcggcggtgt cggccgcggt
     6241 cgaagagatc aaggccggcg aggcctttca gatcgtgccc agccaacgct tcgacctgac
     6301 caccgacgca gacccgctcg acgtctaccg gctgttgcgg cgcagcaacc ccagcccgta
     6361 cctgtacctg ttgcggctgc ccggactcgc ggtggtgggc tccagccccg aggcgctggt
     6421 caccgtgcag aacggacggg ccgagaccca tccgatcgcc ggctcccggc cccgcggacg
     6481 ctccgccgcc gacgacgccg ccatggaagc ccagctgttc gccgacccca aggagcgggc
     6541 cgaacacctg atgctggtcg atctgggccg caacgacctg gggcgggtct gcgcgccggg
     6601 caccgtcgag gtggtcgagt tcatgaaggt gcgccgctac agccacatca tgcatctgga
     6661 ggcggcggtg gtcggcgaac tctcgcccgg acgcagcgcg ctcgacgtca cgctgtcgtg
     6721 ctttcccgcc ggcaccctgt ccggggcgcc gaaggtgcgg gcgatgcaga tcatcgacgg
     6781 cctggaggtc tcacgacgcg gcctctacgg cggcggcgtg ggctacttcg acttcgccgg
     6841 caactccgac gtggcgatag cgattcgcac cgcggtgctc cgcaacggcg ttgcccacgt
     6901 gcaggccggc gccggcatcg tggccgactc ggtgcccgcc agcgaagagc aggaggtgca
     6961 gcacaaggcg gccgcggtgc tgcgggccat cgagggtgcg catcgactgc ggcgcctgaa
     7021 cggggcctga gcgggtgcgc gcgttgtcgc ttgccatgct gtggggcggc gcagggctgg
     7081 cgctggtggc ctccgggcaa ccgtggtgga gcgccggcag cagcagcgaa accggcagcg
     7141 gcgcgacatc cggggcggcc tcggtgctgg tgctggccgc cgcggcagga gcctttctga
     7201 gcagctggct gcgccgcagg gccggccgcc tggtcagttg cctggtggcg ctgctgctgg
     7261 tgggggcggc ggtggtcgca gcgaccgccg gcgcgccggt atcgctgccg ggcagcgcgc
     7321 tgaccaacgc ggccgtcacg gccaccgcct ggcgctgggt ctacctgg
//
LOCUS       DAOSUG010000087         7359 bp    DNA     linear   ENV 10-JUN-2024
DEFINITION  MAG TPA_asm: Micropruina sp. isolate AMR_MDS_2195
            NODE_5004_length_7359_cov_4.318319, whole genome shotgun sequence.
ACCESSION   DAOSUG010000087 DAOSUG010000000
VERSION     DAOSUG010000087.1
DBLINK      BioProject: PRJNA925477
            BioSample: SAMN32864262
            Sequence Read Archive: SRR4236673
KEYWORDS    WGS; ENV; Metagenome Assembled Genome; MAG; Third Party Data; TPA;
            TPA:assembly.
SOURCE      Micropruina sp. (wastewater metagenome)
  ORGANISM  Micropruina sp.
            Bacteria; Bacillati; Actinomycetota; Actinomycetes;
            Propionibacteriales; Nocardioidaceae; Micropruina.
REFERENCE   1  (bases 1 to 7359)
  AUTHORS   Abdulkadir,N., Saraiva,J.P., Zhang,J., Stolte,S., Gillor,O.,
            Harms,H. and Rocha,U.
  TITLE     Genome-centric analyses of 165 metagenomes show that mobile genetic
            elements are crucial for the transmission of antimicrobial
            resistance genes to pathogens in activated sludge and wastewater
  JOURNAL   Microbiol Spectr 12 (3), e0291823 (2024)
   PUBMED   38289113
REFERENCE   2  (bases 1 to 7359)
  AUTHORS   Abdulkadir,N.
  TITLE     Direct Submission
  JOURNAL   Submitted (24-MAR-2023) Environmental Microbiology, Helmholtz
            Center for Environmental Research -UFZ, Permoserstr 15, Leipzig
            04318, Germany
COMMENT     The annotation was added by the NCBI Prokaryotic Genome Annotation
            Pipeline (PGAP). Information about PGAP can be found here:
            https://www.ncbi.nlm.nih.gov/genome/annotation_prok/
            
            ##Genome-Assembly-Data-START##
            Assembly Method        :: SPAdes v. 3.11.1
            Genome Representation  :: Full
            Expected Final Version :: Yes
            Genome Coverage        :: Not Applicable
            Sequencing Technology  :: Illumina MiSeq
            ##Genome-Assembly-Data-END##
            
            ##Genome-Annotation-Data-START##
            Annotation Provider               :: NCBI
            Annotation Date                   :: 05/04/2023 04:57:10
            Annotation Pipeline               :: NCBI Prokaryotic Genome
                                                 Annotation Pipeline (PGAP)
            Annotation Method                 :: Best-placed reference protein
                                                 set; GeneMarkS-2+
            Annotation Software revision      :: 6.5
            Features Annotated                :: Gene; CDS; rRNA; tRNA; ncRNA
            Genes (total)                     :: 2,776
            CDSs (total)                      :: 2,750
            Genes (coding)                    :: 2,714
            CDSs (with protein)               :: 2,714
            Genes (RNA)                       :: 26
            tRNAs                             :: 24
            ncRNAs                            :: 2
            Pseudo Genes (total)              :: 36
            CDSs (without protein)            :: 36
            Pseudo Genes (ambiguous residues) :: 0 of 36
            Pseudo Genes (frameshifted)       :: 2 of 36
            Pseudo Genes (incomplete)         :: 32 of 36
            Pseudo Genes (internal stop)      :: 2 of 36
            ##Genome-Annotation-Data-END##
FEATURES             Location/Qualifiers
     source          1..7359
                     /organism="Micropruina sp."
                     /mol_type="genomic DNA"
                     /submitter_seqid="NODE_5004_length_7359_cov_4.318319"
                     /isolate="AMR_MDS_2195"
                     /isolation_source="Activated sludge of municipal
                     wastewater treatment plants"
                     /db_xref="taxon:2737536"
                     /environmental_sample
                     /geo_loc_name="Canada"
                     /lat_lon="49.25 N 123.1 W"
                     /collection_date="2015-06-16"
                     /metagenome_source="wastewater metagenome"
                     /note="metagenomic"
     gene            complement(76..1704)
                     /gene="rny"
                     /locus_tag="PLF56_04275"
     CDS             complement(76..1704)
                     /gene="rny"
                     /locus_tag="PLF56_04275"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_014846419.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_function: GO:0004521 - endoribonuclease activity
                     [Evidence IEA];
                     GO_process: GO:0006402 - mRNA catabolic process [Evidence
                     IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="ribonuclease Y"
                     /protein_id="HOQ52810.1"
                     /translation="MDSTQQLLALVAVLAIVLIGLTIVVVSILRNQQKAQKQAARRVV
                     EQEKQDRSRLQSLVAKVDPMARKAEGDEIIATARAEAERVRAEAERARLDAEESVRIE
                     RDRVDKLLGDVERRERRMAEREDQHGALQRKLDLRLSDLDATAADLDARRAELAEAEN
                     HRRHELARVAGMTSEEARAELLAQVEHEARLQAVQLSREIESEARRDGEQKARQIIVG
                     AIQRLATDQTTESVVTTVPLPGDEMKGRIIGREGRNIRAFEQTTGVNVMIDDTPESVL
                     LSSFDPVRREVARLTLTELVTDGRIHPARIEEVYERSKRRIDEICLRAAEDAVAAVGL
                     TDLDARLLPTIGALQYRTSYGQNVLKHLIECAQLAGLMAVELQLDPAPARRAAFLHDI
                     GKALTHEVEGSHAKVGADLARRFGEHPDIVNAIEAHHNEVEPKTVIAVLTQAADAISG
                     GRPGARRESLEVYVKRLHRLEEIARAHKGVDKVFAMQAGREIRVMVQPEAVDDAETHV
                     IAKDIAKQVEAELTYPGTIKVTVVRESRVTETAH"
     gene            complement(2079..2615)
                     /locus_tag="PLF56_04280"
     CDS             complement(2079..2615)
                     /locus_tag="PLF56_04280"
                     /inference="COORDINATES: protein motif:HMM:NF014668.2"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_process: GO:0006282 - regulation of DNA repair
                     [Evidence IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="regulatory protein RecX"
                     /protein_id="HOQ52811.1"
                     /translation="MAVAREIALRQLSVRDRTRHELEQALAKKNVPPEAAEQVLDRLT
                     EVGLINDSAFAQAWFDNQQRRQRSTRALRHELRTKGVDAEVVSQASAGVADDVDLSVA
                     RALVAKRLPSLQRVPHELRHRRLAGQLARRGFSSGVVSAVLRELPRDDTDAVGDHYTL
                     GDEALGADPADPADGVGE"
     gene            complement(2651..3697)
                     /gene="recA"
                     /locus_tag="PLF56_04285"
     CDS             complement(2651..3697)
                     /gene="recA"
                     /locus_tag="PLF56_04285"
                     /inference="COORDINATES: protein motif:HMM:TIGR02012.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_component: GO:0005737 - cytoplasm [Evidence IEA];
                     GO_function: GO:0003677 - DNA binding [Evidence IEA];
                     GO_function: GO:0008094 - ATP-dependent activity, acting
                     on DNA [Evidence IEA];
                     GO_process: GO:0006281 - DNA repair [Evidence IEA];
                     GO_process: GO:0006310 - DNA recombination [Evidence IEA];
                     GO_process: GO:0009432 - SOS response [Evidence IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="recombinase RecA"
                     /protein_id="HOQ52812.1"
                     /translation="MAIADREKALEAALAQIEKQHGKGSVMRLGEDVRQPIEIIPTGS
                     IALDVALGIGGLPRGRVVEIYGPESSGKTTVALHVIANAQAAGGICAFIDAEHALDPD
                     YAARLGVDTDALLVSQPDNGEQALEIADMLVRSGALALVVIDSVAALTPRAEIEGEMG
                     DSHVGLQARLMSQALRKMTGALAGAGTTAIFINQLREKIGVMFGSPETTTGGRALKFY
                     SSVRLDVRRIETLKDGSDMVGNRTRVKVVKNKVAPPFKQAEFDIIYGQGISREGSLID
                     LGVETGLVRKAGAWFTYEADQLGQGKENARNYLKKNPEVANEIESRILSHLGIGQPGE
                     VPAGIDPVTGEVEF"
     gene            complement(3899..4093)
                     /locus_tag="PLF56_04290"
     CDS             complement(3899..4093)
                     /locus_tag="PLF56_04290"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_013161200.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="DUF3046 domain-containing protein"
                     /protein_id="HOQ52813.1"
                     /translation="MREAELWARLTHHFGRVQALVWAEGHVIGELGSRTVREALADGV
                     DCKDIWRAVWQNLELPARDR"
     gene            complement(4095..4397)
                     /locus_tag="PLF56_04295"
     CDS             complement(4095..4397)
                     /locus_tag="PLF56_04295"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_012920617.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_function: GO:0003677 - DNA binding [Evidence IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="helix-turn-helix transcriptional regulator"
                     /protein_id="HOQ52814.1"
                     /translation="MIAAEAARPLLMRELLGESLRELRNADQRTLREVSSSARVSLGY
                     LSEIERGQKEPSSELLNAICGALGVPLSYVLRRVSDKFDDRDVVAHLGMHSARVAA"
     gene            complement(4440..4967)
                     /locus_tag="PLF56_04300"
     CDS             complement(4440..4967)
                     /locus_tag="PLF56_04300"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_016455691.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="CinA family protein"
                     /protein_id="HOQ52815.1"
                     /translation="MTERPLAEIVGRTLAERGLTLATSESLTGGLLGATLTALPGASR
                     YYVGGVVVYATEQKARLGGVNPQVLSTHGVISEQTAVEMVMGVQRLTGADYALSASGV
                     AGPDPQEGHAPGEVWIAAVGPTVGPFEPLPVALRYEFDGDRLRIREQTVTTALQMLLQ
                     LLDPAEREQNKQGAR"
     gene            complement(4964..5551)
                     /locus_tag="PLF56_04305"
     CDS             complement(4964..5551)
                     /locus_tag="PLF56_04305"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_014846414.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_component: GO:0016020 - membrane [Evidence IEA];
                     GO_function: GO:0016780 - phosphotransferase activity, for
                     other substituted phosphate groups [Evidence IEA];
                     GO_process: GO:0008654 - phospholipid biosynthetic process
                     [Evidence IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="CDP-alcohol phosphatidyltransferase family
                     protein"
                     /protein_id="HOQ52816.1"
                     /translation="MTTAQPSRLNPANALTLLRLVLVPVFGWMLLSHPHDLWWRLGTT
                     AAFALAIGTDALDGRIARKYNLITDFGKLWDSIADKALTGMAFVGLSILGELPWWVTV
                     LVLLREWGITWMRVAMLKYGVMAANAGGKLKTVLQSIALLLYLPGLPLMPLWLQWIAY
                     VAMAAAVLLTLVTAVPYIREALALRRTGRAEQEKR"
     gene            complement(5548..6936)
                     /gene="rimO"
                     /locus_tag="PLF56_04310"
     CDS             complement(5548..6936)
                     /gene="rimO"
                     /locus_tag="PLF56_04310"
                     /EC_number="2.8.4.4"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_002515571.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_function: GO:0008172 - S-methyltransferase activity
                     [Evidence IEA];
                     GO_function: GO:0016782 - transferase activity,
                     transferring sulphur-containing groups [Evidence IEA];
                     GO_function: GO:0051539 - 4 iron, 4 sulfur cluster binding
                     [Evidence IEA];
                     GO_function: GO:1904047 - S-adenosyl-L-methionine binding
                     [Evidence IEA];
                     GO_process: GO:0018339 -
                     peptidyl-L-beta-methylthioaspartic acid biosynthetic
                     process from peptidyl-aspartic acid [Evidence IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="30S ribosomal protein S12 methylthiotransferase
                     RimO"
                     /protein_id="HOQ52817.1"
                     /translation="MTERVHLVSLGCARNDVDSEELLGRLEAGGFELTDDPATASAIV
                     VNTCGFIDAAKKDSVDTLLAAADLKHDGSVKAVVAVGCMAERYGRELADALPEADAVL
                     GFDAYPDIAERLRWITAGGSVEAHQPHDRRRLLPLAPRERPAAADQVVMPGHAPASGP
                     RVLRRRIGDAPTAALKVASGCDRRCTFCAIPSFRGSYLSRPADELVAEARWLADQGVR
                     ELFLVSENTSSYGKDLRQGPRALERLLGDLAGVDGIEWIRVSYLQPAELRPGMVEGML
                     AVDKVVPYFDLSFQHAAPAVLRRMRRFGDPDSFLALLASIRELAPAAGIRSNVIVGFP
                     GETENDFATLMQFASDARLDALGVFAYSDEDGTEAERLSGKLPDALIAERLETMSTLA
                     QELCDQRAAERIGERVRVLVEEPDGTGRAAHQGPEVDGATRLTGPRPVGSLVNAVVIE
                     ADGIDLIAAEAP"
     gene            complement(7145..>7359)
                     /locus_tag="PLF56_04315"
     CDS             complement(7145..>7359)
                     /locus_tag="PLF56_04315"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_002519407.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=3
                     /transl_table=11
                     /product="DNA translocase FtsK"
                     /protein_id="HOQ52818.1"
                     /translation="LEAATLVVNLQLGSTSMLQRKLRVGFAKAGRLMDILETRGVVGP
                     SEGSKPREVLVKPDDLDEVLVNLRAG"
BASE COUNT         1129 a         2568 c         2416 g         1246 t
ORIGIN      
        1 ctgctgatcg gcctcgggtg ggatcgccta tcccgaggac cggcggcaga cagccggagc
       61 cggtgatgat tcgggtcagt gggcggtttc ggtgacccgc gactcgcgga ccacggtcac
      121 cttgatcgtg ccgggatacg tcaactcggc ctcgacctgc ttggcgatgt ctttggcgat
      181 cacatgggtc tcggcatcgt caacggcctc gggctgcacc atcacccgga tctcgcgacc
      241 ggcctgcatg gcgaacacct tgtcgacgcc cttgtgcgcc cgagcgatct cttcgaggcg
      301 gtgcagccgc ttgacgtaga cctcgaggct ttcgcgccgg gcaccgggac gtccgccact
      361 gatcgcgtcc gccgcctggg tcagcaccgc gatcacggtt ttcggctcca cctcgttgtg
      421 gtgtgcctcg atggcgttga cgatgtcggg atgctcgccg aatctgcggg ccaggtcggc
      481 gccgaccttg gcatgcgagc cttcgacctc gtgggtgagc gccttgccga tgtcgtgcag
      541 aaaggccgct cggcgcgccg gcgccgggtc gagctgcagc tcgacggcca tcaggcccgc
      601 cagctgggca cactcaatca ggtgcttcag cacgttctgg ccgtagctgg tgcggtactg
      661 caaggccccg atggtgggca gcagccgagc gtccagatcg gtcaggccca ccgcggcgac
      721 ggcgtcctcg gcggcccgca ggcagatttc gtcgattcga cgcttgctgc gctcgtagac
      781 ctcttcgatg cgggccgggt ggatgcgtcc gtcggtgacc aactcggtca aggtcagccg
      841 ggccacttcg cggcgcaccg gatcgaacga cgacagcagc acgctctcgg gtgtgtcgtc
      901 gatcatcacg ttgaccccgg tggtctgctc gaaggcccga atgttgcggc cctcgcggcc
      961 gatgatgcgg cccttcatct catcgcccgg gagtggcact gtggtgacca ccgactcggt
     1021 ggtctggtcc gtggccaacc gctggatcgc cccgacgatg atctggcgcg ccttctgttc
     1081 gccgtcgcga cgcgcctcgg actcgatctc gcggctcagt tggacggcct gcagtcgggc
     1141 ctcgtgctcg acctgcgcca ataactcggc gcgcgcctcc tccgatgtca tgccggcgac
     1201 gcgggccaac tcgtggcgcc gatggttctc ggcctcagcg agttccgccc ggcgggcatc
     1261 cagatcggca gcggtcgcgt ccaggtcgct cagcctcaga tcgagcttgc gctgcagtgc
     1321 cccgtgctgg tcttcgcgct cggccattct gcgttcacga cgttcgacgt cgccgagcag
     1381 cttgtcgacc cgatcgcgtt cgatacggac ggactcctcg gcgtcgaggc gcgcacgttc
     1441 agcttcggca cgcacccgct cggcctcggc ccgcgcggtc gcgatgatct cgtcgccctc
     1501 ggcttttcgc gccatcgggt cgaccttggc caccagcgac tgcagccggg accggtcctg
     1561 cttctcttgc tcgacgaccc ggcgggccgc ctgcttctgg gccttctgct gattgcgcag
     1621 gatcgacacc accacgatgg tcaggccgat caggacgatc gcgagcacgg cgacgagcgc
     1681 cagcaactgc tgcgtggaat ccatccgata cctcccgtcg tcacagcaca gtcacaaccc
     1741 agaggtacgc cacacgtaca tatcaacttg tcaatcagca gggcacgcac cacgtcgcgc
     1801 gtaccgtgcc gttccgctcc gcggggtgga cggaacatcc agttacaaag tcgggcctac
     1861 cggcgttgaa aaagtcgatc gaatcggttg atgagtcgtt gacacacctg caggtgaggt
     1921 taggcgcacc aagcccgtgg atcaagctgg acgccttgtc ggacgaatgt tggacaccgt
     1981 ggccgacctg gcccaagtgg cctgggtcac tcttcgtgcg tgggccggag cgtgccactg
     2041 accgggtcgg tgtcgagtgg ttcatcaccg agcgggtact actcgccgac cccgtctgcc
     2101 gggtctgccg ggtcggcgcc gagggcctca tcaccgagtg tgtagtggtc gccgaccgcg
     2161 tcggtgtcgt cccgcggcaa ctcgcgcagc actgccgaga cgacccccga cgagaagccg
     2221 cgtctggcca actgcccggc cagccgccgg tggcgcagct cgtgcggcac gcgttgcagg
     2281 gacggcaggc gtttggccac cagggcccgg gccaccgaca gatcgacgtc gtcggccacc
     2341 ccggcggacg cctgtgacac cacctctgcg tcgacgccct tggtacgcag ctcgtgacgc
     2401 aacgccctgg tggagcgctg ccgtcgctgc tggttgtcga accaggcctg ggcaaaggcg
     2461 gagtcgttga tcaggcccac ctccgtcagg cggtcgagca cctgctcggc cgcttcgggt
     2521 ggcacgttct tcttggccag cgcctgctcg agctcatgcc gggtgcggtc gcgcacgctc
     2581 aactgccgca gagcgatctc gcgtgccacg gccaccggat cggcgtcagg cccgggctgc
     2641 tgccgctcgc tcagaactcg acctcgcccg tgaccgggtc gatgccggcc ggcacctcac
     2701 cgggttgacc gatgcccaga tgcgacagaa tgcgcgactc gatctcgttg gcgacctcgg
     2761 ggttcttctt gaggtagttg cgggcgttct ctttgccctg gcccagctga tcggcctcgt
     2821 aggtgaacca ggcgcccgcc ttgcgcacca ggccggtctc gacacccagg tcgatcaggc
     2881 tgccctcgcg gctgatgccc tggccgtaga tgatgtcgaa ctcggcctgc ttgaacggcg
     2941 gcgccacctt gttcttgacc accttgaccc gggtgcggtt gccgaccatg tcggaaccat
     3001 ctttgagggt ttcgatgcgg cggacgtcga ggcgcaccga ggagtagaac ttgagcgccc
     3061 ggccaccggt ggtggtctca ggtgagccga acatgacgcc gatcttttcg cgcaactggt
     3121 tgatgaagat ggcggtggtg ccggcgcccg ccagcgcgcc ggtcatcttg cgcaacgcct
     3181 ggctcatcaa ccgggcctgc aacccgacgt ggctgtcacc catctcgccc tcgatctcgg
     3241 ctcgtggggt gagtgcggcc accgagtcga tcacgaccag cgccagcgca ccggagcgca
     3301 ccagcatgtc ggcgatctcg agggcctgtt cgccgttgtc tggttggctg accagcagcg
     3361 cgtcggtgtc gacgcccagt ctggccgcgt agtcggggtc gagcgcatgc tcggcgtcga
     3421 tgaaggcaca gatgccgccg gccgcctgcg cgttggcgat cacgtgcaac gcaacagtgg
     3481 tcttgccgct ggattctggg ccatagatct cgaccacccg gccgcgcggc aacccgccga
     3541 tgccgagggc cacgtcgagc gcgatcgaac cggtggggat gatctcgatc ggctgtcgca
     3601 cgtcttcacc gagccgcatc acggagccct tgccgtgctg cttctcgatc tgggccaagg
     3661 ccgcttcgag ggccttctcg cgatctgcaa tcgccatggt gtgtggggtc cttcctggaa
     3721 cggcctgggt cgctgttgct gacttctctt tcaccctacg aaccggcact gacaaaaacg
     3781 tcagaatcga tcccgcttac ggctagacag gcccggtgag tcactcggcc gggtcagcac
     3841 caactgccca aaccctaccg aacgcttgtt cgaaagtgac tcgacacgcc gagttcgctc
     3901 agcggtcgcg agcgggcagt tcgaggttct gccacacggc gcgccagatg tccttgcagt
     3961 ccaccccatc ggccagcgcc tcacggacgg tgcggctacc cagttcaccg atcacatgac
     4021 cctcggccca gaccagcgcc tgtacccgtc cgaaatggtg ggtcaacctg gcccaaagct
     4081 cggcctcacg cacctcaggc ggccacgcgg gccgagtgca tgcccaagtg ggcgaccacg
     4141 tcacggtcgt cgaacttatc gctcactcgg cgcagcacgt acgacagcgg cacgcccagc
     4201 gccccgcaga tcgcgttcaa cagttcactg gagggctctt tttgaccacg ctcgatctcg
     4261 gagaggtagc cgaggctgac ccgtgcggac gaggacacct cgcgcagcgt gcgctggtcg
     4321 gcgttgcgca actcgcgcag cgactcaccc agcagctcac gcatcaacag cggccgcgcg
     4381 gcctcggcag cgatcatgcc cacgactcta cccatcgacg tagttgaaac atcagtggtt
     4441 caacgcgcgc cctgtttgtt ctgttcccgc tcggccgggt cgagcagctg cagcagcatc
     4501 tgcagggcgg tggtgaccgt ctgctcacga atacgcagcc gatcgccgtc gaactcgtag
     4561 cgaagggcga ccggaagcgg ctcgaacggg ccgacggttg ggcctaccgc cgcgatccac
     4621 acctcacccg gcgcgtgtcc ctcctgcggg tccggaccgg ccaccccgga cgccgagagc
     4681 gcatagtcgg cgccggtgag ccgctggacg cccatcacca tctcgaccgc ggtctgttcc
     4741 gagatcacgc cgtgggtgct cagcacctgc gggttcacgc cgcccagccg ggccttctgc
     4801 tcggtggcat agacgaccac gccgccgacg tagtagcgcg aggcaccggg cagtgcggtc
     4861 agggtcgcac ccagcagacc accggtcagc gactcggacg tggccagggt gagtccccgc
     4921 tcggcaaggg tgcggccgac gatctcggcc aatggacgct cggtcatcgc ttctcctgtt
     4981 cggcgcgccc cgtgcggcgc agcgccaggg cttcgcgaat atagggcacc gcggtgacca
     5041 gcgtcagcag caccgcagca gccatcgcca cgtaggcgat ccactgtagc cagagcggca
     5101 tcaggggcag cccgggcagg tacagcagca gtgcgatgct ctgcagcacg gtcttgagct
     5161 tgccgcccgc gttcgccgcc atcaccccgt acttcagcat ggcgacccgc atccaggtga
     5221 tgccccactc gcgcagcagc accagcaccg tgacccacca gggcagttca cccagaatgc
     5281 tcagcccgac gaaagccata ccggtcaacg ccttgtcggc gatcgaatcc cacagcttgc
     5341 cgaagtcggt gatcaggttg tacttgcggg cgatccggcc gtccagcgcg tcggtgccga
     5401 tggccagggc gaatgcggcc gtggtaccca gccgccacca caggtcgtgc ggatggctga
     5461 gcagcatcca accgaacacg ggcaccagca ccagccgcag cagcgtcagt gcgttggccg
     5521 ggttgagccg gctgggctgt gcggtggtca tggcgcctcg gctgcgatca ggtcgatgcc
     5581 gtcggcctcg atgaccacgg cattcaccag gctacctacc ggacgcggcc cggtcagccg
     5641 agtcgcaccg tccacctcgg gaccctggtg cgcggcgcgt ccggtgccgt cgggctcttc
     5701 gaccaggacg cgtacccgct caccgatccg ctccgcggcg cgctgatcgc acagttcctg
     5761 cgccagcgtc gacatcgtct cgagtcgttc ggcgatcagc gcatcgggca gcttgccgct
     5821 cagcctctcg gcctcggttc cgtcctcgtc ggagtaggcg aacacgccca gcgcgtccag
     5881 ccgggcgtcc gaggcgaact gcatcagggt ggcgaaatcg ttctcggtct cgcccggaaa
     5941 ccctacgatc acattgctgc gaatgcccgc cgccggcgcc agttcgcgga tgctcgcgag
     6001 caacgccagg aacgagtcgg gatcgccgaa gcgtcgcatc cggcgcagta ctgccggcgc
     6061 ggcgtgctga aacgacaggt cgaaatacgg aaccaccttg tcgactgcca gcatgccctc
     6121 gaccatgccg ggccgcagtt cggccggctg taggtacgac accctgatcc actcgatgcc
     6181 gtccaccccg gccaggtcgc cgagcagccg ctccagggcc cgcgggccct gacgcagatc
     6241 cttgccgtac gacgaggtgt tctcgctgac caggaacagt tccctgaccc cctgatcggc
     6301 cagccagcgg gcctcggcga cgagttcgtc cgccggccgc gacaggtacg agccgcggaa
     6361 cgatgggatg gcgcagaacg tgcaccggcg atcgcaaccg ctggccacct tgagtgccgc
     6421 cgtgggcgcg tcgccgatgc gccggcgcaa cacccggggg ccgctggcgg gtgcgtgccc
     6481 gggcatgacc acctgatcgg cggccgccgg acgctcgcgg ggcgccagcg gcagcagccg
     6541 gcgacggtcg tgcggctggt gcgcttcgac ggacccgccg gcggtgatcc agcggagtcg
     6601 ctcggcgatg tcggggtagg cgtcgaagcc cagcacggcg tccgcttcgg gcagtgcatc
     6661 ggccagttcg cgtccgtagc gctcggccat gcagcccacg gccacgacgg ccttcaccga
     6721 accgtcgtgt ttgaggtcgg cagcggcgag cagggtgtcg acggagtcct tcttggccgc
     6781 atcgatgaag ccgcaggtgt tcaccacgat ggcgctggcc gtcgcggggt cgtcggtcag
     6841 ctcgaaaccg cctgcctcga gtcggccgag caactcctcg gaatcgacgt cgttgcgcgc
     6901 acagccgagg ctcaccagat ggacgcgctc agtcacctgc gcaatctatc ccagtgcggt
     6961 gtgcgtggtc cgttcgtgcc gggtctcgaa ggtcggcgcg agatcccggc gtccgttggc
     7021 ggggccggtt tcttggtcac cccgggcctg ctccgggtct caaccggtgc gcagatcccg
     7081 tcgttcgcct ggacgactgg ctgggtgcgc cgggaagacg gggcgggacg agacgacagc
     7141 tgggtcaacc ggcgcgcagg ttgaccagca cctcgtccag atcgtcgggc ttcaccagca
     7201 cctcgcgcgg cttggagccc tcggagggac cgaccactcc cctggtctcg aggatgtcca
     7261 tcagccggcc cgccttggcg aacccgaccc gcagcttgcg ttgcagcatc gaggtcgagc
     7321 ccagttgcag gttgaccacc agcgtggccg cttcgagca
//
LOCUS       DAOSUG010000088         7270 bp    DNA     linear   ENV 10-JUN-2024
DEFINITION  MAG TPA_asm: Micropruina sp. isolate AMR_MDS_2195
            NODE_5072_length_7270_cov_6.286209, whole genome shotgun sequence.
ACCESSION   DAOSUG010000088 DAOSUG010000000
VERSION     DAOSUG010000088.1
DBLINK      BioProject: PRJNA925477
            BioSample: SAMN32864262
            Sequence Read Archive: SRR4236673
KEYWORDS    WGS; ENV; Metagenome Assembled Genome; MAG; Third Party Data; TPA;
            TPA:assembly.
SOURCE      Micropruina sp. (wastewater metagenome)
  ORGANISM  Micropruina sp.
            Bacteria; Bacillati; Actinomycetota; Actinomycetes;
            Propionibacteriales; Nocardioidaceae; Micropruina.
REFERENCE   1  (bases 1 to 7270)
  AUTHORS   Abdulkadir,N., Saraiva,J.P., Zhang,J., Stolte,S., Gillor,O.,
            Harms,H. and Rocha,U.
  TITLE     Genome-centric analyses of 165 metagenomes show that mobile genetic
            elements are crucial for the transmission of antimicrobial
            resistance genes to pathogens in activated sludge and wastewater
  JOURNAL   Microbiol Spectr 12 (3), e0291823 (2024)
   PUBMED   38289113
REFERENCE   2  (bases 1 to 7270)
  AUTHORS   Abdulkadir,N.
  TITLE     Direct Submission
  JOURNAL   Submitted (24-MAR-2023) Environmental Microbiology, Helmholtz
            Center for Environmental Research -UFZ, Permoserstr 15, Leipzig
            04318, Germany
COMMENT     The annotation was added by the NCBI Prokaryotic Genome Annotation
            Pipeline (PGAP). Information about PGAP can be found here:
            https://www.ncbi.nlm.nih.gov/genome/annotation_prok/
            
            ##Genome-Assembly-Data-START##
            Assembly Method        :: SPAdes v. 3.11.1
            Genome Representation  :: Full
            Expected Final Version :: Yes
            Genome Coverage        :: Not Applicable
            Sequencing Technology  :: Illumina MiSeq
            ##Genome-Assembly-Data-END##
            
            ##Genome-Annotation-Data-START##
            Annotation Provider               :: NCBI
            Annotation Date                   :: 05/04/2023 04:57:10
            Annotation Pipeline               :: NCBI Prokaryotic Genome
                                                 Annotation Pipeline (PGAP)
            Annotation Method                 :: Best-placed reference protein
                                                 set; GeneMarkS-2+
            Annotation Software revision      :: 6.5
            Features Annotated                :: Gene; CDS; rRNA; tRNA; ncRNA
            Genes (total)                     :: 2,776
            CDSs (total)                      :: 2,750
            Genes (coding)                    :: 2,714
            CDSs (with protein)               :: 2,714
            Genes (RNA)                       :: 26
            tRNAs                             :: 24
            ncRNAs                            :: 2
            Pseudo Genes (total)              :: 36
            CDSs (without protein)            :: 36
            Pseudo Genes (ambiguous residues) :: 0 of 36
            Pseudo Genes (frameshifted)       :: 2 of 36
            Pseudo Genes (incomplete)         :: 32 of 36
            Pseudo Genes (internal stop)      :: 2 of 36
            ##Genome-Annotation-Data-END##
FEATURES             Location/Qualifiers
     source          1..7270
                     /organism="Micropruina sp."
                     /mol_type="genomic DNA"
                     /submitter_seqid="NODE_5072_length_7270_cov_6.286209"
                     /isolate="AMR_MDS_2195"
                     /isolation_source="Activated sludge of municipal
                     wastewater treatment plants"
                     /db_xref="taxon:2737536"
                     /environmental_sample
                     /geo_loc_name="Canada"
                     /lat_lon="49.25 N 123.1 W"
                     /collection_date="2015-06-16"
                     /metagenome_source="wastewater metagenome"
                     /note="metagenomic"
     gene            complement(93..404)
                     /locus_tag="PLF56_04320"
     CDS             complement(93..404)
                     /locus_tag="PLF56_04320"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_013865238.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_function: GO:0003677 - DNA binding [Evidence IEA];
                     GO_function: GO:0003700 - DNA-binding transcription factor
                     activity [Evidence IEA];
                     GO_process: GO:0006355 - regulation of DNA-templated
                     transcription [Evidence IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="metalloregulator ArsR/SmtB family transcription
                     factor"
                     /protein_id="HOQ52819.1"
                     /translation="MTLPLADEVFDALGDHTRRQLLRAIGDEPVAVGVLAERVAVGRP
                     AVSKHLKVLQGAGLVSHHSVGTRNLYALAPEGYAVAQRWFADTWTAALTAFARHLEQQ
                     K"
     gene            complement(458..1315)
                     /locus_tag="PLF56_04325"
     CDS             complement(458..1315)
                     /locus_tag="PLF56_04325"
                     /inference="COORDINATES: ab initio
                     prediction:GeneMarkS-2+"
                     /note="Derived by automated computational analysis using
                     gene prediction method: GeneMarkS-2+."
                     /codon_start=1
                     /transl_table=11
                     /product="hypothetical protein"
                     /protein_id="HOQ52820.1"
                     /translation="MSWPAPTGDPIRPQRDGAQPTPGQAPGDQTSATTPPPPTWAQVQ
                     PGAGYPPPTVPLPTQPLPTTPMPAPLTPPAEQPTGAVATAAGPGHIDTLSAPAVTPPG
                     LAKLSEKTGQPVRPWTIWASAVLMFGGAITVTVGLLLAMWVMASPWVEVADGWTKDDK
                     FNEATWLTAQFPSEPAGGWRVFFAIVCCVIAVAVAGTAAVIGYYAFAGYGWTRIGGLV
                     ALGVSLLSLLLTPIAAVTIGFVALGAAALWLPATRPFFARWQAVRHPQTAYSQPIQNV
                     FYGPLPRYR"
     gene            complement(1356..1961)
                     /locus_tag="PLF56_04330"
     CDS             complement(1356..1961)
                     /locus_tag="PLF56_04330"
                     /inference="COORDINATES: protein motif:HMM:NF037150.2"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_function: GO:0003677 - DNA binding [Evidence IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="TetR family transcriptional regulator"
                     /protein_id="HOQ52821.1"
                     /translation="MTDSPAKPRGRRPGRADTRGTICRAALTLFSTIGYDKVSLRAIA
                     READVDPALIHHYFSSKSDLFAQTVLDLPLEAGQVVSDVLNGPREQIGERAVAAFLAA
                     YDSPESGRDRFTAMLRSAVAADIVQRPLSEFMSKEVFGPIAEQLGHRDYRLRAQLAVS
                     LVLGMALSRYILRLQALEAASDRDLVRGLGRAMQEILVESW"
     gene            2184..2831
                     /locus_tag="PLF56_04335"
     CDS             2184..2831
                     /locus_tag="PLF56_04335"
                     /inference="COORDINATES: protein motif:HMM:TIGR01509.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_function: GO:0016787 - hydrolase activity [Evidence
                     IEA];
                     GO_process: GO:0008152 - metabolic process [Evidence IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="HAD-IA family hydrolase"
                     /protein_id="HOQ52822.1"
                     /translation="MNFVAPKTVLFDLDGTLADTVPLIVATFKRTVSQALGWEPTLAQ
                     CKEWIGRSLTETFTTLAPEVADELTARYLEWNLANHQAYVRPFDGVGALIGGLRASGR
                     PFGVVTSKRHASALVSLECVGLTGQIPLLVTEEDTATHKPSPEPLLHALNQMGADAAD
                     AVYVGDAIVDMQAARAAGVRGIGVTWGAASAEELRAAEPTAVVHSVGELQTLLGL"
     gene            complement(2857..5796)
                     /locus_tag="PLF56_04340"
     CDS             complement(2857..5796)
                     /locus_tag="PLF56_04340"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_015068804.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_function: GO:0004553 - hydrolase activity, hydrolyzing
                     O-glycosyl compounds [Evidence IEA];
                     GO_process: GO:0005975 - carbohydrate metabolic process
                     [Evidence IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="glycoside hydrolase family 2 TIM barrel-domain
                     containing protein"
                     /protein_id="HOQ52823.1"
                     /translation="MNGDWRFRLLPGAPGGAVAVLPDGGAPDAIAEDGFDDSGWNELP
                     VPSHWVLHGGGRYGRPIYTNVQFPFPIEPPFVPDDNPTGDHRRRFALPADFAGAASVL
                     LRFDGVESRYRVWLNGVEIGIGSGSRLAQEFDVTHAVRPGENLLVVRVHQWSAASYLE
                     DQDQWWLPGIFRDVTLLARPAGALDDVWLRTDYHAGRGSIRPEIVAADDAYPMTLRVP
                     ELGVEVTWHSASEVSGIDAGPVQPWSAELPRLYDATVASTGETVRLRLGFRTVEIAGD
                     RLLVNGERLVFHGMNRHETHPDRGRVFDEDWARADLLLMKRFNVNAIRTSHYPPHPRL
                     LDLADELGLWVILECDLETHGFEPGGWRDNPSDDPAWREAMLDRIERTVERDKNHPSI
                     VLWSLGNESGTGQNLAAMASWVHGRDPDRPVHYEGDYTGAYTDVYSRMYSSVPETASI
                     GRDDSGALLFGCSASESARQRSKPFLLCEYAHAMGNGPGALDQYEELVERYPRLHGGF
                     IWEWRDHGLRTHTADGIEFFGYGGDFGEVVHDGNFVMDGMVLSDGTPTPSLHEFAQVA
                     APFAIEVADVLTIRSRRHSADSADVVFAWSAEADGEPLASGELDVPPLEPGASVAVAR
                     PTLPELPDGAGELWLNVTARLVAPTAWAPAGHPLVTAQGLIARAPEATLRWSPAPRDP
                     ATGGLGPGLPDILGPARFEAGRLVELAGEPVRGPRLALWRAPTDNDRGAAFGSYQDAD
                     PTLPPWRATPAPPWDVRWREAGLDRMVPRVVSASGSASRLRVRTRWAAANSRLAVICD
                     EHWWLDERGLALVVELEPSTGWDLIWPRLGVRFDLPTGVDGASWFGTGPLESYPDSLR
                     AARVGRFSAALDELTVGYARPQESGHRSDLRSLTVRRSGADWLRIDTGPDAAGRLPGF
                     TLTPHTAEEVDRAAHPQELPPSSATRLYLDAAQHGLGSRACGPDVWPAQALAPRAASL
                     RLRFAPG"
     gene            complement(5886..6794)
                     /locus_tag="PLF56_04345"
     CDS             complement(5886..6794)
                     /locus_tag="PLF56_04345"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_013861053.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_process: GO:0008643 - carbohydrate transport [Evidence
                     IEA];
                     GO_process: GO:0055085 - transmembrane transport [Evidence
                     IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="carbohydrate ABC transporter permease"
                     /protein_id="HOQ52824.1"
                     /translation="MSTTTVSKPARTAGSNDHSNRSAGRSAGMTIVVTGLLVIVALYF
                     IVPVYWVLVAASKTSEDLFSTFGFWFAPNFALWENLTQVVTYDNGIFVRWFLNSVLYA
                     GVGSLIATYLAAAGGYALAKYKFKGSNLIFGIILGGVLVPGTATALPLFLLFSQLGMA
                     NTYWSVLIPSLVSPFGLFLCRIYADATVDDALLEAARLDGASELRIFHTIGLNILTPA
                     LVTVFLFQLIGIWNNYFLPLVMLADSQLYPITLGLNNWLSQVDRLPEFYELTTGGVLL
                     SVIPLVIAMVVLQRFWRGGLTEGAVK"
     gene            complement(6791..>7270)
                     /locus_tag="PLF56_04350"
     CDS             complement(6791..>7270)
                     /locus_tag="PLF56_04350"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_013861054.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_function: GO:0042626 - ATPase-coupled transmembrane
                     transporter activity [Evidence IEA];
                     GO_function: GO:0140359 - ABC-type transporter activity
                     [Evidence IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="sugar ABC transporter permease"
                     /protein_id="HOQ52825.1"
                     /translation="LSPIVDVAGWMGLEVDFLGADTVLWSIANIVTWSYAGYNMLIIV
                     AQLKAIPNELYEAARVDGATPFQVAWRIQIPLIVPALMLTTVFSIIGTLQLFAEPQVL
                     SKVAPAIDSQFTPNLSAFTTAFAYNDYNVAAAQAVLIAVVAFVLSFVFLRFTNREDS"
BASE COUNT         1154 a         2502 c         2624 g          990 t
ORIGIN      
        1 gcgctccagg ggccaccaca ggccgatctc ggtggtgaac acggcgaagg cgcggcctgc
       61 cgtggcggcg acgatctgcc ggtgcagcgg cgtcatttct gctgctcgag atgccgggcg
      121 aaggcggtca gcgcggccgt ccaggtgtcg gcgaaccagc gctgggccac cgcgtagccc
      181 tcgggggcca gcgcgtacag gttgcgggtg cccacgctgt ggtggctgac caacccggcg
      241 ccctgcagca ccttcaggtg cttgctcacc gccggccggc cgacggccac gcgctcggcg
      301 agcaccccta cggcaacggg ctcgtcgccg atcgcgcgca acaactgacg cctggtgtgg
      361 tcgcccaggg cgtcgaacac ctcgtcggca agcggtaacg tcattcggtt actctacagc
      421 aaccgttgct gcagggtaac cattgccgaa ggacggttca gcgatagcgc ggcagcgggc
      481 cgtagaagac gttctgaatc ggctgcgagt aggccgtctg tgggtgccgc acggcctgcc
      541 agcgggcgaa gaacggcctt gtcgccggca gccacagcgc cgcggccccg agtgcaacga
      601 agccgatcgt gacggccgcg atcggggtga gcaacagcga cagcagcgac accccgagcg
      661 cgaccagccc gccgatgcgc gtccagccgt agccggcgaa cgcgtagtag ccgatcaccg
      721 ctgcggtgcc ggccacggcc accgcgatta cgcaacagac gatcgcgaag aacacccgcc
      781 agccgccggc gggctcggac gggaactgcg cggtcagcca ggtggcctcg ttgaacttgt
      841 cgtctttggt ccagccgtcg gccacctcga cccagggcga ggccatcacc cacatggcga
      901 gcagcaggcc cacggtgacg gtgatcgcgc cgccgaacat cagcaccgcc gaggcccaga
      961 tcgtccacgg ccgcacgggt tggccggtct tctcgctcaa cttggccaac ccgggcgggg
     1021 taacggccgg agcgctgagc gtgtcgatgt gccccggacc cgccgcggtc gcgacggccc
     1081 cggtcggctg ttcggcgggc ggggtcagcg gcgccggcat gggcgtggtg ggcagcggct
     1141 gagtcggcag gggcacggtc gggggcgggt agccggcgcc cggctgaacc tgcgcccagg
     1201 tcggcggcgg cggggtcgtt gcggacgtct ggtcgccggg cgcctgccca ggggtcggct
     1261 gcgctccgtc ccgttggggg cggatcgggt caccggttgg tgctggccag ctcatcgtcc
     1321 tcctgaatcc gcgaccagcc taccgagccg gcgaatcacc agctctcgac gagaatctct
     1381 tgcatcgccc ggcccaggcc acgcaccagg tcgcggtcgg acgccgcctc gagcgcctgc
     1441 aatctgagga tgtagcggct gagcgccatg cccagcacga ggctcaccgc gagctgggcc
     1501 cgcaggcgat agtcacgatg accgagctgc tcggcgatcg ggccgaacac ctctttcgac
     1561 atgaactcgc tgagcggccg ctgcacgatg tcggccgcca ccgcggagcg gagcatggcg
     1621 gtgaaccggt cacggccgct ctcggggctg tcgtaggcgg ccagaaaggc ggccacggcc
     1681 cgctcgccga tctgttcgcg cgggccgttc agcacgtcgc tcaccacctg gccggcttcg
     1741 agcggaaggt cgagcacggt ctgggcgaac aggtcggact tgctggagaa gtagtggtga
     1801 atcagcgccg ggtcgacgtc ggcctcgcgc gcgatggcgc gcagcgacac cttgtcgtag
     1861 ccgatcgtcg agaacagggt gagcgcggcg cggcagatgg tgccgcgggt atcggcgcgg
     1921 cccggccggc gcccgcgtgg cttcgcgggg gagtccgtca tgcgcttcct ctctcacctg
     1981 ccggcggacg cggggggtcc cgtcgcggcc cgaccagcca ccggccggtc ccatcgaagc
     2041 cggcccgcgg tggccgccgt cccacgttgc tcaggttcgg cgtccttgct caggtatgaa
     2101 cctatgcaac gaacccaggg ctgagcaacg tcccgcgagg gtggtgaaca cgggacgccc
     2161 gccgggcaga ttaggctgac ccggtgaatt tcgtggcgcc gaagaccgtt ctgttcgacc
     2221 tcgacggcac gcttgccgac accgtcccgc tgatcgtcgc gaccttcaag cgcacggtgt
     2281 cgcaggcgct gggctgggaa ccgacgctcg cgcagtgcaa ggagtggatc gggcggtcgc
     2341 tgaccgagac gttcaccaca ctggcgcccg aggtggccga cgagctgacc gcgcgctacc
     2401 tggagtggaa cctggccaac caccaggcct atgtgcggcc cttcgacggg gtcggtgccc
     2461 tgatcggggg actgcgggcg tccggccgac cgttcggggt agtcacgtcc aagcggcacg
     2521 catcggccct ggtcagcctg gagtgcgtcg gcctgaccgg gcagatcccg ctgttggtca
     2581 ccgaggagga caccgccacc cacaagccct cgcccgaacc gctgctgcat gccctgaacc
     2641 agatgggtgc ggacgccgcg gacgccgtct acgtcgggga cgccatcgtc gatatgcagg
     2701 cggcccgggc ggccggtgtc cggggcatcg gggtcacctg gggagcggcg agcgccgagg
     2761 agctccgtgc cgccgaaccg accgcggtcg tgcacagcgt cggcgaactg cagaccctgc
     2821 tcggcctgta acggacgcag cgccgcacgg gccacttcag cccggggcga accggagccg
     2881 cagggacgcc gcccgggggg cgagcgcctg cgccggccac acgtccgggc cacacgcccg
     2941 cgagccgaga ccgtgctggg ccgcgtccag atacagccgg gtggcgctcg acggcggcag
     3001 ctcctgggga tgcgcggcgc ggtcgacctc ctcggcggtg tgcggcgtga gcgtgaagcc
     3061 gggaagccgg cccgcggcgt ccggaccggt gtcgatgcgc agccagtcgg ccccggatcg
     3121 gcgcacggtc agcgagcgca ggtcgctgcg gtgcccgctc tcctggggac gtgcgtaccc
     3181 gacggtcaac tcgtcgagcg ccgcgctgaa gcggcccacc cgggcggccc ggaggctgtc
     3241 cggatatgac tccagcggac ccgtcccgaa ccaggaggca ccgtcgacgc cggtcggcag
     3301 gtcgaagcgg acgccgagcc gcggccagat caggtcccac ccggtgctgg gctccaactc
     3361 gacgaccagc gccaggccgc gctcgtcgag ccaccagtgc tcgtcgcaga tcacggcgag
     3421 acgtgagttc gccgccgccc accgggtgcg gacccgcagc cggctcgccg acccggacgc
     3481 ggacaccacc cgcgggacca tccggtccag gccggcctcc cgccagcgga cgtcccaggg
     3541 tggggctggc gtggcgcgcc acggcggcag ggtggggtcg gcgtcctggt agctgccgaa
     3601 cgcggcgccg cggtcgttgt cggtaggggc ccgccacaac gccaaccgag gtccacggac
     3661 cggttcgccg gccaactcga cgaggcgtcc ggcctcgaac cgggcgggcc cgaggatgtc
     3721 cggcagcccc ggcccgagcc cgccggtggc cgggtcgcgc ggtgccggcg accatcgcag
     3781 ggtcgcctcc ggcgcccgcg cgatcaggcc ctgggccgtc accagcgggt ggccggccgg
     3841 cgcccacgca gtcggcgcga ccagccgcgc cgtcacgttc agccacagct cgcctgcgcc
     3901 gtcgggcagt tcgggcagcg tcggacgcgc cacggccacc gatgcgccgg gttccagcgg
     3961 gggcacgtcc aactcgccgg aggccagcgg ctcgccgtcg gcctcggccg accaggcgaa
     4021 caccacgtcc gccgagtcgg cgctgtgccg ccggctgcgg atcgtgagca cgtcggccac
     4081 ctcgatcgcg aacggcgcgg cgacctgggc gaactcgtgc agcgaggggg tgggggttcc
     4141 gtcggagagc accatgccgt ccatcacgaa gttgccgtcg tgcaccacct cgccgaagtc
     4201 gccgccgtag ccgaagaact cgatgccgtc ggcggtgtgc gtgcgaagtc cgtggtcgcg
     4261 ccattcccag atgaacccgc cgtgcagccg cggataccgc tcgaccagct cttcgtactg
     4321 atcgagcgcg cccgggccgt tgcccatcgc gtgggcgtac tcgcagagca ggaacggctt
     4381 ggagcgctgc cgcgccgatt cggacgccga acagccgaac agcagcgcgc cggagtcgtc
     4441 gcggccgatc gacgcggtct cgggaaccga ggagtacatc cgcgagtaca cgtcggtgta
     4501 cgcgccggtg tagtcgccct cgtagtgcac cggccggtcg gggtcgcggc catgcaccca
     4561 ggacgccatc gcggccagat tctgcccggt tcctgactcg ttgcccagcg accacagcac
     4621 gatcgacgga tggttcttgt cccgctcgac ggtgcgttcg atgcggtcga gcatcgcctc
     4681 ccgccaggcc gggtcgtcgc tcggattgtc ccgccagcca cccggctcga agccgtgggt
     4741 ctccaggtcg cactccagga tcacccagag gccgagctcg tcggccaggt cgagcagccg
     4801 cggatgcggg ggatagtggc tggtgcggat cgcgttcacg ttgaaccgct tcatcagcag
     4861 caggtcggca cgggcccagt cctcgtcgaa gacccggccg cgatcggggt gcgtctcgtg
     4921 ccggttcatt ccgtggaaga ccagccgttc gccgttgacc agcagccggt cgccggcgat
     4981 ctcgacggtg cggaagccga gccgcagccg cacggtctca ccggtggagg cgaccgtggc
     5041 gtcgtagagc cgcggcagct ccgccgacca gggctgcacc gggccggcgt cgatacccga
     5101 cacctcggac gccgaatgcc aggtcacctc gacgcccagc tcgggcaccc gcagggtcat
     5161 cgggtaggcg tcgtcggcgg ccacgatctc gggacggatc gagccccgtc cggcgtggta
     5221 atcggtgcgc agccagacgt cgtccagggc gcccgcgggg cgggcgagca gcgtcacgtc
     5281 gcggaagatg cccggcagcc accactggtc ctggtcctcc aggtagctgg ccgccgacca
     5341 ctggtgcacg cgcaccacca gcaggttctc gccggggcgg acggcatggg tcacgtcgaa
     5401 ctcctgcgcc aaccggctgc ccgacccgat gccgatctcg accccgttca gccagacccg
     5461 gtagcgcgac tcgaccccgt cgaagcgcag cagcaccgac gccgcgccgg cgaagtccgc
     5521 gggcaacgcg aagcgccggc ggtggtcgcc ggtcgggttg tcgtccggca cgaacggggg
     5581 ttcgatcggg aacgggaact gcacattggt gtagatcgga cgcccgtagc gtccgccacc
     5641 gtgcagcacc caatgggacg ggacggggag ctcgttccag ccggagtcgt cgaagccgtc
     5701 ctcggcgatc gcgtcgggtg ctccgccgtc cggcagcacc gcgacggcgc caccgggggc
     5761 gccgggcagc agccggaacc gccagtcccc gttcaacgac agttccggcg cgtcgctgtc
     5821 cagccgggaa cgggcggggg cccgggtacc ggacccaggc ccccgatcga gcagatagtg
     5881 ggcggctact tgaccgctcc ttccgtgagc ccaccgcgcc agaagcgctg caggacaacc
     5941 atcgcgatga ccagcgggat caccgacagc aggacgccgc cggtggtcag ctcgtagaac
     6001 tcgggcaacc gatcgacctg gctcagccag ttgttcaggc cgagcgtgat cggatacagc
     6061 tgggagtctg ccagcatcac cagcggcagg aagtagttgt tccagatgcc gatgagctgg
     6121 aagaggaaga cggtcaccag ggccggggtg aggatgttga gcccgatggt gtggaagatc
     6181 cgcagctcac tggcgccgtc caggcgagcg gcctcgagca gtgcgtcgtc cacggtcgcg
     6241 tcggcgtaga tccgacaaag gaacagcccg aacggtgaga ccagcgacgg gatcagcacg
     6301 ctccagtagg tgttggccat acccagctgg ctgaacagca ggaacagcgg caacgcggtg
     6361 gcggtgcccg gtaccagaac accgcccagg atgatgccga agatcaggtt gctgcccttg
     6421 aacttgtact tggctagggc gtacccgccg gccgccgcca gataggtggc gatcaacgac
     6481 cccactccgg catacaggac cgagttgagg aaccaacgca cgaagatgcc gttgtcatag
     6541 gtgacgacct gggtcaggtt ctcccatagc gcgaagttcg gggcgaacca gaacccgaac
     6601 gttgagaaca ggtcctcgct ggtcttggac gcggccacca gaacccagta aacgggcacg
     6661 atgaagtaca acgcgacgat cacgagcaga ccggtgacca cgatggtcat gcccgcggag
     6721 cgccccgcgg agcggttgga gtgatcgttg gatccggccg tccgggccgg cttcgaaacg
     6781 gtggtggtac tcacgagtcc tcccggttgg tgaagcggag gaagacgaac gagagcacga
     6841 acgcgaccac cgcgatgagc accgcctgcg cggcggcgac gttgtagtcg ttgtaggcga
     6901 aggctgtggt gaaggcggac aggttgggag tgaactggct gtcgatcgcc ggagcgacct
     6961 tgctcagcac ctggggttcg gcgaagagct gcagcgtgcc gattatagag aacaccgtgg
     7021 tgagcatcag ggccgggacg atcagcggga tctggatgcg ccaggcgacc tggaagggcg
     7081 tggcgccgtc gacgcgtgcc gcctcgtaga gttcgttcgg gatcgccttc agctgggcga
     7141 cgatgatcag catgttgtag cccgcgtagc tccaggtgac gatgttggcg atcgaccaca
     7201 gcacggtgtc ggcgccgagg aagtccacct cgagacccat ccagccggcg acgtcgacga
     7261 tcgggctgag
//
LOCUS       DAOSUG010000089         7205 bp    DNA     linear   ENV 10-JUN-2024
DEFINITION  MAG TPA_asm: Micropruina sp. isolate AMR_MDS_2195
            NODE_5120_length_7205_cov_4.244895, whole genome shotgun sequence.
ACCESSION   DAOSUG010000089 DAOSUG010000000
VERSION     DAOSUG010000089.1
DBLINK      BioProject: PRJNA925477
            BioSample: SAMN32864262
            Sequence Read Archive: SRR4236673
KEYWORDS    WGS; ENV; Metagenome Assembled Genome; MAG; Third Party Data; TPA;
            TPA:assembly.
SOURCE      Micropruina sp. (wastewater metagenome)
  ORGANISM  Micropruina sp.
            Bacteria; Bacillati; Actinomycetota; Actinomycetes;
            Propionibacteriales; Nocardioidaceae; Micropruina.
REFERENCE   1  (bases 1 to 7205)
  AUTHORS   Abdulkadir,N., Saraiva,J.P., Zhang,J., Stolte,S., Gillor,O.,
            Harms,H. and Rocha,U.
  TITLE     Genome-centric analyses of 165 metagenomes show that mobile genetic
            elements are crucial for the transmission of antimicrobial
            resistance genes to pathogens in activated sludge and wastewater
  JOURNAL   Microbiol Spectr 12 (3), e0291823 (2024)
   PUBMED   38289113
REFERENCE   2  (bases 1 to 7205)
  AUTHORS   Abdulkadir,N.
  TITLE     Direct Submission
  JOURNAL   Submitted (24-MAR-2023) Environmental Microbiology, Helmholtz
            Center for Environmental Research -UFZ, Permoserstr 15, Leipzig
            04318, Germany
COMMENT     The annotation was added by the NCBI Prokaryotic Genome Annotation
            Pipeline (PGAP). Information about PGAP can be found here:
            https://www.ncbi.nlm.nih.gov/genome/annotation_prok/
            
            ##Genome-Assembly-Data-START##
            Assembly Method        :: SPAdes v. 3.11.1
            Genome Representation  :: Full
            Expected Final Version :: Yes
            Genome Coverage        :: Not Applicable
            Sequencing Technology  :: Illumina MiSeq
            ##Genome-Assembly-Data-END##
            
            ##Genome-Annotation-Data-START##
            Annotation Provider               :: NCBI
            Annotation Date                   :: 05/04/2023 04:57:10
            Annotation Pipeline               :: NCBI Prokaryotic Genome
                                                 Annotation Pipeline (PGAP)
            Annotation Method                 :: Best-placed reference protein
                                                 set; GeneMarkS-2+
            Annotation Software revision      :: 6.5
            Features Annotated                :: Gene; CDS; rRNA; tRNA; ncRNA
            Genes (total)                     :: 2,776
            CDSs (total)                      :: 2,750
            Genes (coding)                    :: 2,714
            CDSs (with protein)               :: 2,714
            Genes (RNA)                       :: 26
            tRNAs                             :: 24
            ncRNAs                            :: 2
            Pseudo Genes (total)              :: 36
            CDSs (without protein)            :: 36
            Pseudo Genes (ambiguous residues) :: 0 of 36
            Pseudo Genes (frameshifted)       :: 2 of 36
            Pseudo Genes (incomplete)         :: 32 of 36
            Pseudo Genes (internal stop)      :: 2 of 36
            ##Genome-Annotation-Data-END##
FEATURES             Location/Qualifiers
     source          1..7205
                     /organism="Micropruina sp."
                     /mol_type="genomic DNA"
                     /submitter_seqid="NODE_5120_length_7205_cov_4.244895"
                     /isolate="AMR_MDS_2195"
                     /isolation_source="Activated sludge of municipal
                     wastewater treatment plants"
                     /db_xref="taxon:2737536"
                     /environmental_sample
                     /geo_loc_name="Canada"
                     /lat_lon="49.25 N 123.1 W"
                     /collection_date="2015-06-16"
                     /metagenome_source="wastewater metagenome"
                     /note="metagenomic"
     gene            694..1209
                     /locus_tag="PLF56_04355"
     CDS             694..1209
                     /locus_tag="PLF56_04355"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_013861098.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="ACT domain-containing protein"
                     /protein_id="HOQ52826.1"
                     /translation="MATLVLSVIGDDRAGLVNALADVISAHGGNWERSQLAELAGKFA
                     GIVVVGVPDERSDELAAALQPLAGLLEVAIHPGLQSKPAPAAGQVQIDLIGNDHPGIV
                     RDVSGVLASHGLSIERLLTETREAPMAGGLLFEAHLTAPLAADHDLVALQSDLEHLAS
                     EILVDISLAAG"
     gene            1426..2232
                     /gene="yaaA"
                     /locus_tag="PLF56_04360"
     CDS             1426..2232
                     /gene="yaaA"
                     /locus_tag="PLF56_04360"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_015581947.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="peroxide stress protein YaaA"
                     /protein_id="HOQ52827.1"
                     /translation="MLVLLSPAKSLDFESPLPIRKHTEPRLLEQSRQLIEVMRGKSAS
                     EIASLMHISDELAALNAQRYADFDPPFTMRNARAAVLAFAGDVYQGMAVRQRWDARDY
                     TEAQKTVRTLSGLYGVLRPLDLMQPYRLEMGLPIATDRGANLYQFWGARITHLLQADL
                     EQSPGAPVLVNLASDEYARSVDFDAFDRPAISPRFEDTNTRGTRSVVSFFAKRARGAM
                     ASWLVRERVRTPGTIRRFAEDGYRYDRAASSPDRPVFVRRFEDRPTMADR"
     gene            complement(2510..2875)
                     /locus_tag="PLF56_04365"
     CDS             complement(2510..2875)
                     /locus_tag="PLF56_04365"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_013862163.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="VOC family protein"
                     /protein_id="HOQ52828.1"
                     /translation="MIDHYGINVADLAASTRFYDTVLAVLGFTRQMDFGEAVGYGTEG
                     HPDFWVSSAATGASNRESHLAFAAADRAAVEAFFAAAQSLGAEVLHAPREWPEYHPGY
                     YAAFVRDPDGNNVEAVVHH"
     gene            complement(3358..4254)
                     /locus_tag="PLF56_04370"
     CDS             complement(3358..4254)
                     /locus_tag="PLF56_04370"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_011754407.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="uracil-DNA glycosylase"
                     /protein_id="HOQ52829.1"
                     /translation="MSAPGGTRPVPPEPHRPSSFPADVVQGRRFPHPVTGVLFPSPAP
                     PGTGWPGDPATAATPVAHDAPTVLALAGSSGDLDELEARVSVCRACPRLVAWREQVAQ
                     QRRAAFADQPYWGRPAPSFGDPRAAVLIVGLAPAAHGANRTGRMFTGDRSGDWLYAAL
                     YRAGYANQPTSTHAGDGLALTGIRIVAAVRCAPPDNKPLPIERATCASWLDRDLLLSA
                     GRLRSLLALGSIAWDATLAAARRLGWTVPRPKPRFGHGAEAVLMAGDRPIRLVGSYHV
                     SQQNTFTGRLTEAMLDDAIGRL"
     gene            complement(4251..5114)
                     /locus_tag="PLF56_04375"
     CDS             complement(4251..5114)
                     /locus_tag="PLF56_04375"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_012920159.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="exodeoxyribonuclease III"
                     /protein_id="HOQ52830.1"
                     /translation="MRLATWNVNSVKQRVPRLLPWLDQRCPDVVCLQETKMTDAAFAD
                     LLAEPLAQRGYEFASHGQGQWNGVALLSRVGLLEVQRDFADMPGFPGPEARAISATCA
                     GLRVHSLYVPNGRVPDSDHYHYKLAWLAALRDSVATGSQQVALCGDVNIAPADADVFD
                     PAAYAGHTHVTAPERAALQALTDLGLHDVVRERWPTQQVFSYWDYRAGMFHMNLGMRI
                     DLVLADAATTARVTAAFVDRQARKGTGPSDHAPVIVDLDEAPDGDIGPMVPPPSAGRY
                     RKGSKKLPQSA"
     gene            5220..6020
                     /locus_tag="PLF56_04380"
     CDS             5220..6020
                     /locus_tag="PLF56_04380"
                     /inference="COORDINATES: ab initio
                     prediction:GeneMarkS-2+"
                     /note="Derived by automated computational analysis using
                     gene prediction method: GeneMarkS-2+."
                     /codon_start=1
                     /transl_table=11
                     /product="hypothetical protein"
                     /protein_id="HOQ52831.1"
                     /translation="MRVCEACGQRFDEPLDRCVRCGRSLVAPSVAGHLSRTSIAGAAG
                     TSSFAAAHAVGSADTSASEGERAAQADAAPTPASEPEGEGPGPGFAPGHTAVSPRVHG
                     ADAATTPSAPSSPEWDPDATTRFTPIYASRSRAKNTAALVGLAIGVVAAVTSLAGLIA
                     QSVVTGPANPRGSTPTSASPTPLSTVVPRNATVCTPELARSSDTNCTVAQRVFSAVRT
                     LGTDLPDSFRVTITDPGTDKNATFVCSIEAWIQCVGDDQAVVYVRRLV"
     gene            complement(6245..6436)
                     /locus_tag="PLF56_04385"
                     /pseudo
     CDS             complement(6245..6436)
                     /locus_tag="PLF56_04385"
                     /inference="COORDINATES: protein motif:HMM:NF012669.2"
                     /note="incomplete; partial in the middle of a contig;
                     missing N-terminus; Derived by automated computational
                     analysis using gene prediction method: Protein Homology."
                     /pseudo
                     /codon_start=1
                     /transl_table=11
                     /product="D-beta-D-heptose 1-phosphate
                     adenosyltransferase"
     gene            complement(6479..6976)
                     /locus_tag="PLF56_04390"
     CDS             complement(6479..6976)
                     /locus_tag="PLF56_04390"
                     /inference="COORDINATES: protein motif:HMM:NF012521.2"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="histidine phosphatase family protein"
                     /protein_id="HOQ52832.1"
                     /translation="MRTLHLLRHAEAQWQSPGPDGDHARPLSSRGVQQARGVGAYLLR
                     EATVDLVVASSSTRTRQTAEALDLGVPVWSRDEIYNAGAQQILQQVRAIPEGCNCVVI
                     VGHAPGIPALAHLLADESSVAAARAVIAYRYPPATLATLHVHGSWAKLRTASLATARI
                     APEPG"
     gene            complement(6973..>7205)
                     /locus_tag="PLF56_04395"
     CDS             complement(6973..>7205)
                     /locus_tag="PLF56_04395"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_002512977.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_component: GO:0005737 - cytoplasm [Evidence IEA];
                     GO_function: GO:0000287 - magnesium ion binding [Evidence
                     IEA];
                     GO_function: GO:0004427 - inorganic diphosphate
                     phosphatase activity [Evidence IEA];
                     GO_process: GO:0006796 - phosphate-containing compound
                     metabolic process [Evidence IEA]"
                     /codon_start=3
                     /transl_table=11
                     /product="inorganic diphosphatase"
                     /protein_id="HOQ52833.1"
                     /translation="IPVADQRQDYLTELKDLPQLTLLEIEHFFAVYKDLEPGKSVEGA
                     VWVGRTEAEKEIFQSWKRASGTDFENEFTRTE"
BASE COUNT         1098 a         2514 c         2471 g         1122 t
ORIGIN      
        1 ttccggcgaa cgccgggatc ttctggttcc gtcatccagg cgaacgccgg gatctcctgc
       61 ttccgtcatc caggcgaacg ccgggatctt cgtcggtgga gacctcctgt ttccgtcgtt
      121 ccggcgaacg ccgggatctt cgtcggtgga gacctccagt ttccgtcatt ccggcgaacg
      181 ccgggatctt cgtcggcgga gacctcctgt ttccgtcatc ccggcgaacg ccgggatctc
      241 cccgaagcgc tccttccaat tgatgccggg gactccgttg gtcgagcttg tcggtccccg
      301 tctgccgaac tcccctgggt tgcgttgggt tgccgccgag tgggggtgca ccccgtcccg
      361 ccccggcaag ccctctcggc tccgcaggct ccgccgagaa gtgtgccgac gcccgaccac
      421 tccctgacgg ggtgcacccc cactccgctt cacggttgcg cgaggtccgg ggccgatcgt
      481 cgtccctggt cgagccctgg ccctcaggcg atctcgagat cgccgctgga tggcgggtta
      541 cctggacgat ttcgtgttgc aacccgaaag tggacggctt tcccgccatc gtgcagcgat
      601 ctcgagatcg cttcggcctc attgatgggt tcctgatgct gcgaccgccg gccgcttcat
      661 cgagttggtc agggactgca ctagcatccg ggcatggcca cccttgtgct ttccgtgatc
      721 ggtgatgatc gcgccggact cgtcaacgcg ctcgcggacg tgatcagcgc ccacggcggc
      781 aactgggagc gtagccaact ggcagaactg gcgggcaagt tcgccggcat cgtggtggtc
      841 ggcgtgcccg atgaacgctc ggacgaactg gctgccgcac tacagccgct ggcgggactg
      901 ctcgaggtgg cgatccaccc gggtctccag tccaagccgg cgccggccgc cggtcaggtg
      961 cagatcgacc tgatcggcaa cgaccacccg ggcatcgtcc gcgacgtgtc gggcgtgctg
     1021 gccagtcacg gcctcagcat cgagcgactg ctcaccgaga cccgtgaggc gccgatggcg
     1081 ggcggtctgc tcttcgaggc gcacctcaca gccccgctcg cggccgacca tgacctcgtc
     1141 gcgctgcaga gcgatctcga acacctggcc agcgagattc tggtcgacat cagcctcgcg
     1201 gccggctgac gttacccgct actcccaccg ctcggctccg gggatgatcc gcagccaggc
     1261 ttccacctcg tcattagcgc gcgaaatggg ccgaattgcc caggacgtcg ccgggcgccg
     1321 actgaaaccg agcaggggtc gcggcgcccc ggacggcggc catgctgggc cgaacggagg
     1381 ccctcggaac ggccgcgcgg gcgaaggttg gctacccttc gtgacgtgct ggttctgctc
     1441 tcgcccgcga agtcgctcga cttcgagtcc ccactgccca tccgcaagca caccgagccc
     1501 cgactgctgg agcagtcgag gcaactgatc gaggtgatgc ggggcaaatc ggcgtccgag
     1561 atcgcgagcc tgatgcacat ctcggacgag ttggcggcgc tcaacgcgca gcgctacgcc
     1621 gacttcgacc cgccgttcac catgcgcaac gctcgcgcgg ccgtgctggc gttcgccggc
     1681 gacgtgtatc agggcatggc ggtgcgacaa cgctgggacg cccgcgacta caccgaggcc
     1741 cagaagaccg tgcgcacgct gtcggggctg tacggcgtgc tgcgccccct cgacctgatg
     1801 cagccctatc gacttgagat gggcctgccg atcgccaccg accgcggcgc caacctgtac
     1861 cagttctggg gtgcccgaat cacgcacctg ctgcaggccg acctggagca gtcgcccgga
     1921 gcccctgtgc tggtcaacct ggcctcggac gaatacgccc gctcggtcga cttcgacgcg
     1981 ttcgaccggc ccgcgatctc gccccgcttc gaggacacca acacccgcgg cacacgcagc
     2041 gtggtgtcgt tcttcgccaa gcgcgctcgc ggcgcgatgg cctcgtggct ggtgcgcgag
     2101 cgggtacgca cccccggtac gatccgcagg ttcgccgagg acggctaccg ctacgaccgg
     2161 gccgcgtcga gcccggaccg gccggttttc gtgcggcggt tcgaggaccg ccccacgatg
     2221 gccgaccggt gatgcgcgcc cgatcgggct ggggaaccgc tgatcaatcg gtgggccgag
     2281 cctgacgaga cccatccgat cagggtttcg actccgacca ggttttggac tccgaccagg
     2341 gccttcgacg ggtgggctca accaacggat tgcgtgtcga ccagggatac ccaggactca
     2401 ccccggtgga tccgcgctca gcccggcgcc ccctggagca ggcagcgtgc tgcggcagcg
     2461 tggagcacac tgtgaagccg tggtgtggag tcgcggtcta caaccaggct cagtggtgca
     2521 cgaccgcctc gacgttgttg ccgtcggggt cgcggacgaa ggccgcgtag tagcccgggt
     2581 ggtactcggg ccactcgcga ggggcgtgca acacctcggc gcccaacgac tgcgccgccg
     2641 cgaagaacgc ctcgaccgcg gctcggtcgg ccgccgcgaa cgccaggtgc gactcgcggt
     2701 tggacgcacc ggtggcggcc gagctgaccc agaagtcggg atggccctcg gtgccgtacc
     2761 cgacggcctc accgaagtcc atctgccggg tgaaaccaag caccgccagc acggtgtcgt
     2821 agaagcgggt cgaggcggcc aggtcggcca cgttgatgcc gtagtgatca atcatgcgaa
     2881 cagtctgtcg cccgcctccg acactttccg cgcagtcggg gcaaccgcag tgacgccgtc
     2941 tcggccacag cgcgatggcc agagaaccga cccacggcga ccgcggatca ccgctcagcg
     3001 ctcaccgatc gccaacgccg accgggtgac gggttcgcca tggctgagcg tggcgcgggg
     3061 ccgccatggc gcccggattg ggcggcgtcg gaggcgtgga tcggtcgatc cgggttcgcc
     3121 atcggttgag cgtggcccgc gccgccagga cgtccggagt tggccgtgtc gcaagcgtgg
     3181 atcggtcgat ccggccgcca ttcggttgag cgtggcgcgc gccgccatgg cgtccggatg
     3241 tgaccgcgtc gcgcgtggat cggtcgatcc tgcttcacca tcggttgagc cagcgccgtg
     3301 gcttccggcc atgcccgcgc agcaggcgca cgccggtggc gacggggtcc gcccgaatca
     3361 gagccggccg atcgcatcgt ccagcatggc ctcggtgagc cgtccggtga aggtgttctg
     3421 ctggctgacg tggtagctgc cgaccaggcg gatcggacgg tccccggcca tcagcaccgc
     3481 ttcggcacca tgcccgaacc gcggtttggg gcgtggcacc gtccacccca gccgacgggc
     3541 cgccgccagg gtggcgtccc aggcgatcga ccccaacgcc agcagtgagc gcagtcggcc
     3601 ggcgctgagc agcaggtcgc gatcgagcca ggacgcgcac gtggcccgct cgatcggcag
     3661 cggtttgttg tcgggcggtg cgcagcgcac cgccgccacg atgcggatgc cggtcagcgc
     3721 caagccgtcg ccggcgtgcg tcgaggtggg ttgattggcg tagccggccc ggtacagcgc
     3781 ggcgtacagc cagtcacccg agcggtcgcc ggtgaacatg cgcccggtgc ggttggcccc
     3841 gtgcgcggcc ggagcgagcc cgacgatcag caccgccgcc cgcggatcgc cgaacgaggg
     3901 cgccggacgt ccccagtacg gctgatcggc gaaggctgcc cggcgctgtt gcgccacctg
     3961 ctcacgccag gccaccagcc gtgggcaggc ccggcacacc gacacccgtg cctccagctc
     4021 gtcgagatca ccggacgagc cggcgagcgc cagcacggtg ggcgcgtcgt gcgccacggg
     4081 cgtcgcggcg gtcgcggggt caccgggcca gcccgtgccg ggcggcgcgg gggagggaaa
     4141 caacaccccc gtcaccgggt gcggaaagcg ccgaccctga acgacatcag ccggaaagct
     4201 gctcggacga tgcggctccg gagggaccgg acgcgtccca ccgggcgcgc tcatgcggat
     4261 tgcggcagct tcttactgcc cttgcgatat cggcccgccg aggggggcgg caccatcggc
     4321 ccgatgtcgc cgtcgggagc ctcatcgagg tcgacgatca ccggagcgtg atcgctgggt
     4381 ccggtgccct tgcgggcctg ccggtcgacg aatgcagccg tcacgcgggc cgtcgtcgcc
     4441 gcgtcggcca gcaccagatc gatgcgcatg cccagattca tatggaacat gcccgcgcga
     4501 tagtcccagt aactgaacac ctgttgcgtg ggccagcgct cgcgcaccac atcgtgcagc
     4561 ccgagatcgg tcagcgcctg cagcgccgct cgctcggggg cggtcacgtg ggtatgcccg
     4621 gcataggccg ccggatcgaa cacatcggca tcggccggtg cgatgttcac atcgccgcac
     4681 agcgccacct gctgcgaccc ggtcgccacg ctgtcgcgca gggcggccag ccaggcgagc
     4741 ttgtagtggt agtggtccga atcgggtacc cggccgttcg gtacgtacag gctgtgcacc
     4801 cgcagcccgg cacacgtggc gctgatcgcg cgcgcctcgg ggccgggaaa gccgggcatg
     4861 tcggcaaagt cgcgctgcac ctcgagcaaa ccgacccgcg acagcagcgc gacgccgttc
     4921 cactggccct gcccgtgtga ggcgaactcg tagccgcgtt gcgccagcgg ctcggcgagc
     4981 aggtcggcga aggccgcatc ggtcatcttg gtctcttgca ggcagaccac atcgggacac
     5041 cgctggtcga gccagggcag cagtcgcggc acgcgttgct tcaccgagtt cacgttccag
     5101 gtcgccaacc gcatgctccg aggttaacgc cccgccaccc acaggaaccc cgcggaaccg
     5161 tttcgcagcg tgtgccagaa tcgagcacgc gaccccgtcc gcctgcgatc ggaatgtcaa
     5221 tgcgtgtctg cgaggcctgc gggcagcggt tcgacgaacc gctcgaccgt tgtgttcgtt
     5281 gtggacgctc attggtcgcc ccgtcggtcg ccggccacct gagccgcacg tcgatcgccg
     5341 gcgcggcggg aacctcgagt ttcgctgcgg cgcacgccgt cggctcggcc gatacctctg
     5401 cgtcggaagg ggagcgagcg gcacaggctg acgctgcgcc gacgcccgcg tccgagccgg
     5461 agggtgaggg ccccggccct ggttttgcac ccgggcacac cgccgtatcg cctcgggttc
     5521 acggtgcgga cgccgccaca acgccgtccg caccgtccag tcccgaatgg gaccccgatg
     5581 cgaccacccg gttcacgccg atctacgcca gccgcagccg ggccaagaac accgccgccc
     5641 tggtggggct ggccatcggc gtggtggcgg ccgtcaccag tctcgcgggg ctgatcgccc
     5701 agtcggtggt gaccgggcct gcaaacccac gtggctcgac gccgacgtcg gcctcgccga
     5761 cccccctcag caccgtggtg ccgcgcaacg ccaccgtctg cacgcccgag ctggcgcgca
     5821 gcagcgacac caactgcacc gttgcccagc gggtattctc ggccgtgcgc accctgggca
     5881 ccgacctgcc cgactcgttt cgggtgacga tcaccgatcc aggaaccgac aagaacgcca
     5941 cgttcgtgtg cagcatcgag gcctggatcc aatgcgtcgg cgatgatcag gcggttgttt
     6001 acgtgcgtcg actcgtctag gagtctgctg aacggtgttg ttctgagcga ggacgtgccc
     6061 gcgggatgag ttgagccccc agatctgagg ttcgatgggt caacactcag ccgagaactg
     6121 ctttctcacc acctgagtgt gggtgacgag gggacggacg gtcggacgcg ccagtagcga
     6181 ggggcgcgcc ttcccgtgga gcgatggacg gggtgtgaca agaagtggac cagcaatcgg
     6241 ctgattaggc acggatgacg tcaagtcccg ttgcgacgag atcgtctgcc aagtcagggt
     6301 cgacgcctgc gtcggtgatc agggtgtcca cgacgtccag ggggacgaca gtcatcagct
     6361 cgtcccggcc gatcttggtg tgatcggcaa ggacgatcac ccgccgcgca gccctcgcca
     6421 gcgcgcgctt cacctcagcc tcgcggtccg taccgtccct ggcgcttggg ccgcggaatc
     6481 agcccggttc gggggcgatc cgcgcagtag ccaggctggc cgtgcgcaac ttcgcccacg
     6541 acccgtgcac gtgcagggtg gccagggtgg ctggcgggta gcggtaggcg atcaccgcgc
     6601 gagccgccgc aactgatgat tcgtcggcca gcaggtgggc cagcgcggga atgccgggag
     6661 catgcccgac gatcaccaca cagttacagc cctcgggaat ggcccgcacc tgctgcagga
     6721 tctgctgcgc acccgcgttg tagatctcat cgcgcgacca cacgggcaca cccagatcga
     6781 gcgcctcggc ggtctgtcgg gtgcgcgtcg acgacgaggc caccaccagg tcgacggtcg
     6841 cctcgcgcag caggtaggca cccaccccgc gagcctgctg cacgccgcgc gaactcagcg
     6901 gacgggcatg gtcaccgtcg ggcccggggg attgccactg agcttcggcg tgccgcagca
     6961 ggtgcagggt tctcactcgg tgcgggtgaa ttcgttctcg aagtcggtgc ccgaggcgcg
     7021 cttccaactc tggaagatct ctttctcggc ctcggtgcgt ccgacccaga ccgcaccttc
     7081 gaccgacttg cccggctcga ggtccttgta cacggcgaag aaatgctcga tctcgagcag
     7141 ggtgagctgg ggcaggtcct tgagttcggt caggtagtcc tggcgttggt cggcgaccgg
     7201 aatgc
//
LOCUS       DAOSUG010000090         7203 bp    DNA     linear   ENV 10-JUN-2024
DEFINITION  MAG TPA_asm: Micropruina sp. isolate AMR_MDS_2195
            NODE_5122_length_7203_cov_4.094432, whole genome shotgun sequence.
ACCESSION   DAOSUG010000090 DAOSUG010000000
VERSION     DAOSUG010000090.1
DBLINK      BioProject: PRJNA925477
            BioSample: SAMN32864262
            Sequence Read Archive: SRR4236673
KEYWORDS    WGS; ENV; Metagenome Assembled Genome; MAG; Third Party Data; TPA;
            TPA:assembly.
SOURCE      Micropruina sp. (wastewater metagenome)
  ORGANISM  Micropruina sp.
            Bacteria; Bacillati; Actinomycetota; Actinomycetes;
            Propionibacteriales; Nocardioidaceae; Micropruina.
REFERENCE   1  (bases 1 to 7203)
  AUTHORS   Abdulkadir,N., Saraiva,J.P., Zhang,J., Stolte,S., Gillor,O.,
            Harms,H. and Rocha,U.
  TITLE     Genome-centric analyses of 165 metagenomes show that mobile genetic
            elements are crucial for the transmission of antimicrobial
            resistance genes to pathogens in activated sludge and wastewater
  JOURNAL   Microbiol Spectr 12 (3), e0291823 (2024)
   PUBMED   38289113
REFERENCE   2  (bases 1 to 7203)
  AUTHORS   Abdulkadir,N.
  TITLE     Direct Submission
  JOURNAL   Submitted (24-MAR-2023) Environmental Microbiology, Helmholtz
            Center for Environmental Research -UFZ, Permoserstr 15, Leipzig
            04318, Germany
COMMENT     The annotation was added by the NCBI Prokaryotic Genome Annotation
            Pipeline (PGAP). Information about PGAP can be found here:
            https://www.ncbi.nlm.nih.gov/genome/annotation_prok/
            
            ##Genome-Assembly-Data-START##
            Assembly Method        :: SPAdes v. 3.11.1
            Genome Representation  :: Full
            Expected Final Version :: Yes
            Genome Coverage        :: Not Applicable
            Sequencing Technology  :: Illumina MiSeq
            ##Genome-Assembly-Data-END##
            
            ##Genome-Annotation-Data-START##
            Annotation Provider               :: NCBI
            Annotation Date                   :: 05/04/2023 04:57:10
            Annotation Pipeline               :: NCBI Prokaryotic Genome
                                                 Annotation Pipeline (PGAP)
            Annotation Method                 :: Best-placed reference protein
                                                 set; GeneMarkS-2+
            Annotation Software revision      :: 6.5
            Features Annotated                :: Gene; CDS; rRNA; tRNA; ncRNA
            Genes (total)                     :: 2,776
            CDSs (total)                      :: 2,750
            Genes (coding)                    :: 2,714
            CDSs (with protein)               :: 2,714
            Genes (RNA)                       :: 26
            tRNAs                             :: 24
            ncRNAs                            :: 2
            Pseudo Genes (total)              :: 36
            CDSs (without protein)            :: 36
            Pseudo Genes (ambiguous residues) :: 0 of 36
            Pseudo Genes (frameshifted)       :: 2 of 36
            Pseudo Genes (incomplete)         :: 32 of 36
            Pseudo Genes (internal stop)      :: 2 of 36
            ##Genome-Annotation-Data-END##
FEATURES             Location/Qualifiers
     source          1..7203
                     /organism="Micropruina sp."
                     /mol_type="genomic DNA"
                     /submitter_seqid="NODE_5122_length_7203_cov_4.094432"
                     /isolate="AMR_MDS_2195"
                     /isolation_source="Activated sludge of municipal
                     wastewater treatment plants"
                     /db_xref="taxon:2737536"
                     /environmental_sample
                     /geo_loc_name="Canada"
                     /lat_lon="49.25 N 123.1 W"
                     /collection_date="2015-06-16"
                     /metagenome_source="wastewater metagenome"
                     /note="metagenomic"
     gene            complement(<1..233)
                     /locus_tag="PLF56_04400"
     CDS             complement(<1..233)
                     /locus_tag="PLF56_04400"
                     /inference="COORDINATES: ab initio
                     prediction:GeneMarkS-2+"
                     /note="Derived by automated computational analysis using
                     gene prediction method: GeneMarkS-2+."
                     /codon_start=1
                     /transl_table=11
                     /product="hypothetical protein"
                     /protein_id="HOQ52834.1"
                     /translation="MTMQSPQAGPRTAQVPQSILTMFIGAVWGALRTVFDRVFGRRGE
                     AKPSEGTPSVPCMTFLPAQPTIDDLGIPLATVT"
     gene            226..450
                     /locus_tag="PLF56_04405"
     CDS             226..450
                     /locus_tag="PLF56_04405"
                     /inference="COORDINATES: ab initio
                     prediction:GeneMarkS-2+"
                     /note="Derived by automated computational analysis using
                     gene prediction method: GeneMarkS-2+."
                     /codon_start=1
                     /transl_table=11
                     /product="hypothetical protein"
                     /protein_id="HOQ52835.1"
                     /translation="MVTALLGGDEVLGREVSGAPTPGVMTLQGDEVSALRAMTEAGLL
                     PPLRLVRSVSRRPPVELNDDRWFESDHDVV"
     gene            437..1534
                     /locus_tag="PLF56_04410"
     CDS             437..1534
                     /locus_tag="PLF56_04410"
                     /inference="COORDINATES: protein motif:HMM:NF013073.2"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="NlpC/P60 family protein"
                     /protein_id="HOQ52836.1"
                     /translation="MTSFRIFSELVRWLPHGDLLVDRLEAMVRLRTVVRGGLVALSGL
                     AVVLAGPAALPAKADPTLDDAKTKLDAIEQQQSELGEQWSEVKLQLQQGKQKVATLNA
                     DIANQQRKVDALRTQAQQVALTRFQNRGVDITIQLVTAPDPDSFLSDMSTMGKVDQNM
                     NSLLQDYQAQQANLTDLQRSARDQVTTMATEEKRLDALNDQLNAKIKEGQQLIDRLTE
                     QERQRLNAGDGSVSRDDARDALIDDGSGDSRALAAVKYAMSKVNGSQYVWGAEGPTGF
                     DCSGLMLAAYRSVGVSLPHSSRAQFGVGRPVSKDELKPGDLLFFYSPIHHVGMYIGNG
                     LFVHARNVRDDLDVTRLDSYPAYQGARRVLG"
     gene            1512..2615
                     /locus_tag="PLF56_04415"
     CDS             1512..2615
                     /locus_tag="PLF56_04415"
                     /inference="COORDINATES: ab initio
                     prediction:GeneMarkS-2+"
                     /note="Derived by automated computational analysis using
                     gene prediction method: GeneMarkS-2+."
                     /codon_start=1
                     /transl_table=11
                     /product="hypothetical protein"
                     /protein_id="HOQ52837.1"
                     /translation="MPAAFSADRFPSGGAARGLDLSRRRLLLSAPLVLTVPLLGGCAT
                     AVPTSTHLADALGSRLSAGDRAGFYALFAGSAGAQAVALRMFDNLLDSGATIAAASSD
                     RLSVTWHLPGEPPVVSLATVTLAGAVVDAIEPVSTGTEWLSDPMTVHAEASLVVASAR
                     QDESARWLTAARAGLAALATVWPAGQRRTEPLIVLAPSDLVGFARYAGADAQTTAAVT
                     VVPGTTASEGVRVVVNPVAATNAVTDAATLTHEAVHAAMGSPRLTGTPGWLLEGIAEA
                     LTARAHPSVAATNAAAARAAVAHGLPNALPDLSSADPSGYALAQVAVDAMVAHVGWTD
                     VFDEARRRSTPAGGRIADAQVLTWYRDELARLR"
     gene            complement(2677..3141)
                     /locus_tag="PLF56_04420"
     CDS             complement(2677..3141)
                     /locus_tag="PLF56_04420"
                     /inference="COORDINATES: ab initio
                     prediction:GeneMarkS-2+"
                     /note="Derived by automated computational analysis using
                     gene prediction method: GeneMarkS-2+."
                     /codon_start=1
                     /transl_table=11
                     /product="hypothetical protein"
                     /protein_id="HOQ52838.1"
                     /translation="MSPRDAEDARFEELMAREFPEGLVPTERARPRLEAPIGPDPTEA
                     EAAEQHAAATDDEADQSAATDGFRSWAPPDERDEPFVPPPAPPAARWSSAGIAGTVLV
                     ALPLVLVLIASFGVHLPMIVTALGGLGFAVGVVLLLIRLRHRPPTDGDGAVV"
     gene            complement(3138..3884)
                     /locus_tag="PLF56_04425"
     CDS             complement(3138..3884)
                     /locus_tag="PLF56_04425"
                     /inference="COORDINATES: protein motif:HMM:NF023568.2"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="alpha/beta fold hydrolase"
                     /protein_id="HOQ52839.1"
                     /translation="MQVHEFAEPIVPSGSGDVGVLFLHGFTASPWTLREWATRTAADG
                     YRVSVPRLPGHGTSWRELEVTDGRDWYAAAERAFLDLRASCQMVFVAGLSMGGALALR
                     LAEHHPDDVAGLVLVNPALHGNPQLALVPWAKHLIRTTNSIGSSIRKADVTEHAYPRT
                     PLAAVHALMRLWSDVRARLDLVHCPVLLYRSELDSVVPSASKDVVVRQVSSDEIIEVV
                     LTESDHVAPLDNDADVIVAGTLDFLARHSR"
     gene            3990..4742
                     /locus_tag="PLF56_04430"
     CDS             3990..4742
                     /locus_tag="PLF56_04430"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_014847069.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="lysophospholipid acyltransferase family protein"
                     /protein_id="HOQ52840.1"
                     /translation="MLYTLFKNVAFRPVIKYLFGARVVGERNIPSAGGVILASNHLDA
                     GDTVVLPAMIKRKVTFPAKAELFKGNRGLFSKLVAWFLTAVGQVPLDRSGGRASLDGL
                     GPVLQVLADGGCVGIYPEGTRSPDGRLYKGKTGVARMALAARVPIVPVAMQNSTLTKK
                     FGLPWIDHPVVVVGEPLDFSEYFDRGDEPRVLRWVTDQVMAAIQSLSGQTYVEAYGTS
                     VKSGSLTKAEADARILERPGSNTRKPDALPPN"
     gene            5137..6528
                     /locus_tag="PLF56_04435"
     CDS             5137..6528
                     /locus_tag="PLF56_04435"
                     /EC_number="2.5.1.54"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_002513958.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_function: GO:0003849 - 3-deoxy-7-phosphoheptulonate
                     synthase activity [Evidence IEA];
                     GO_process: GO:0009423 - chorismate biosynthetic process
                     [Evidence IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="3-deoxy-7-phosphoheptulonate synthase class II"
                     /protein_id="HOQ52841.1"
                     /translation="MSTQIPTLEQLQALSPVQQPTYADTQQVERVTAQLRTRPPLVFA
                     GECDDLRAKIADVAAGRAFILQGGDCAETFDTGTALDIRGKLRVLLSMAVVLTYAAQV
                     PVVKIGRLAGQYAKPRSKDTEIRGGVELPAYRGDAVNGFDFSAAARVHDPNRLMEVYN
                     HSAATLNLIRAFVTGGFADLRAVHSWNQDFVRNSGVEARYEALADEIERALAFMVACG
                     VDKEAFRTVDFYSSHEALLLPYEHAMTRIDSRTGTPYNTSAHMVWIGERTRQVDGAHV
                     QFARHLRNPIGVKIGPSMTGDEVVALADALDPQHEPGRLTLISRMGAGKVRTALPPLV
                     RAVEATGRSVAWVCDPMHGNTFETANGYKTRAFSQVVDEMNGFFDVHEELGTWPGGVH
                     MELTGDDVTECVGGVADALAEADLPNRYETACDPRLNRNQSLELAFLIAERLTDGRIK
                     RTNPVQEYRAEDF"
     gene            6637..>7203
                     /locus_tag="PLF56_04440"
     CDS             6637..>7203
                     /locus_tag="PLF56_04440"
                     /inference="COORDINATES: ab initio
                     prediction:GeneMarkS-2+"
                     /note="Derived by automated computational analysis using
                     gene prediction method: GeneMarkS-2+."
                     /codon_start=1
                     /transl_table=11
                     /product="hypothetical protein"
                     /protein_id="HOQ52842.1"
                     /translation="MRRNWLIFATAAATLLVLAYPVNNNIRYEVGVHRVSGYSIAETF
                     SHRPLAFRLVSAAQSWLPDLASESLAAPGSLARAWLFEAGFRLVAALVAAAAAVLLTV
                     GLRRRLGSAAWAYGLAAYAGLFFTAPATGEPDWWAAVVAVAAVGTALLARESTGGSMA
                     GALLAVVALLKISSLPIALAAVMLIAAFD"
BASE COUNT         1110 a         2462 c         2501 g         1130 t
ORIGIN      
        1 aacgtgaccg tggcaagggg aatgcccagg tcgtcgatcg tgggttgggc cggcaggaac
       61 gtcatgcacg gcacgctagg cgtgccctct gacggttttg cttcgccccg ccgtccgaac
      121 acgcgatcga aaactgtccg aagcgcgccc cagactgcac ctatgaacat ggtgaggatc
      181 gattgcggca cctgcgcggt gcgagggccg gcctgcggcg actgcatggt caccgcactc
      241 ctgggtggcg atgaggtgct gggccgcgaa gtgtccggcg cccccacccc gggggtgatg
      301 accctgcagg gcgacgaggt gagtgccctg cgggccatga ccgaggcggg gttgctgccg
      361 ccgctgcgac tggtacgatc ggtgtcccga cgccccccgg tcgagttgaa cgacgaccgc
      421 tggtttgaat ctgatcatga cgtcgtttag gattttctca gaattggtgc gctggcttcc
      481 ccacggcgac ctgctcgtcg atcgtctgga ggcgatggtg cgtttgcgca cagttgttcg
      541 tggtggcttg gtggcgctct cggggctggc ggtggtgctg gccggtcccg cagcgttgcc
      601 ggccaaggca gaccccaccc tcgacgatgc caagaccaag ctggacgcga tcgagcagca
      661 gcagagcgag ctcggtgagc agtggtccga ggtcaagctg cagcttcagc agggcaagca
      721 gaaggtcgcc acgctcaacg cagacatcgc caaccagcaa cgcaaggtcg acgcactgcg
      781 cacccaggcc cagcaggtcg cgctgacccg gttccagaac cgtggggtcg acatcaccat
      841 ccagctggtc accgcacctg atcccgattc gttcctgtcc gacatgtcca ccatgggcaa
      901 ggtcgatcag aacatgaact cgctgttgca ggactatcag gcccagcagg ccaacctcac
      961 cgacctgcag cgctcggccc gcgaccaggt caccaccatg gccaccgaag agaagcgcct
     1021 cgacgcactc aacgaccaac tcaacgccaa gatcaaagag ggtcaacagc tgatcgaccg
     1081 gctgaccgag caggagcgtc agcggctcaa cgcgggcgac ggctccgtct cgcgcgatga
     1141 cgcccgcgat gcgctgatcg acgacggctc gggcgactcg cgcgccctgg ccgcggtcaa
     1201 gtacgcgatg tcgaaggtca acggcagcca gtacgtgtgg ggggccgagg gtcccaccgg
     1261 gttcgactgc tccggattga tgctcgccgc ctaccgctcc gtcggcgtgt cgttgccgca
     1321 ctcgtcgcgg gcccagttcg gcgtcggccg gccggtctcg aaggacgaac tcaagcccgg
     1381 cgacctgctg ttcttctaca gcccgattca ccacgtcggc atgtacatcg gcaacggact
     1441 gttcgtgcat gcccgtaacg tgcgcgacga cctcgacgtc acccggctcg attcgtaccc
     1501 ggcctaccag ggtgcccgcc gcgttctcgg ctgatcggtt cccttcgggc ggcgcggcgc
     1561 gcgggctcga tctgtcgcgg cgcaggctgc ttctgagtgc gccgctggtg ctgaccgtgc
     1621 cgctgctcgg gggttgcgca accgcggtgc ccacctccac gcacctcgcg gacgccctgg
     1681 gctcgcgact ttccgccggc gatcgggccg gcttctacgc cctgttcgca ggctcggccg
     1741 gagcgcaggc ggtggccttg cgcatgttcg acaacctgct cgattccggc gccacgatcg
     1801 ccgccgcgtc gtccgatcgg ctgagcgtga cgtggcacct gcccggcgaa ccgccggtgg
     1861 tcagccttgc cacggtcacc ctggcgggcg ctgtcgtcga tgcgatcgaa ccggtgagca
     1921 ccggcaccga atggttgagc gatccgatga ccgttcatgc cgaggcgtcg ctggtggtgg
     1981 ccagtgcgcg acaagacgag tcggcacggt ggctgaccgc ggcacgagct ggcctggcag
     2041 cgctggcgac ggtgtggccg gcggggcagc gtcgcaccga accgctgatc gtgctggctc
     2101 cgtccgatct ggtcggcttc gcccggtacg cgggcgcgga cgcgcagacg acggcagcgg
     2161 tgaccgtcgt gccgggcacc acggccagcg agggtgtccg cgtggtggtc aacccggttg
     2221 ctgccacgaa cgccgtaacc gacgcggcga ccctgaccca cgaggccgtc cacgcggcga
     2281 tgggttcgcc acggctgacc ggcacgcccg ggtggctgct cgaaggcatc gccgaggccc
     2341 tgacggcccg ggcgcacccg agcgtcgcgg ccaccaacgc agcagcagcg cgggctgcgg
     2401 tcgcgcacgg cctgccgaat gcgctgcccg acctgagcag cgcagacccg tccggctacg
     2461 ccctggcgca ggtggcggtg gacgccatgg tggcccatgt cgggtggacg gacgtgttcg
     2521 acgaggcgcg gcggcgcagc acaccggcgg gcggacgcat cgcggatgcc caggtgctca
     2581 cctggtaccg cgacgagctg gcgcggctgc gctagtcgag tcgtggccgt cgacgctctg
     2641 tcggacgcca cctttccgtg gtttcagagg gctggcctag accaccgcgc cgtccccgtc
     2701 ggtgggcgga cggtgtcgca gcctgatcag cagcagcacc acgccgactg caaaaccgag
     2761 gccgcccagt gccgtgacga tcatcggcag atgcacgccg aacgaggcga tcagcaccag
     2821 taccagtggg agtgcgacca gcacggtccc ggcgatgccg gcgctgctcc agcgtgcggc
     2881 ggggggagcc ggcggcggca cgaacggttc gtccctctca tcgggcggag cccagctgcg
     2941 aaagccgtcc gtggctgccg actggtcggc ctcgtcgtcg gtggcggccg cgtgttgctc
     3001 ggcagcctcg gcttcggtcg ggtctggccc gatcggggcc tccagccgcg gccgggcccg
     3061 ctcggtcggc accagcccct cgggaaactc ccgggccatc aactcctcga accgggcgtc
     3121 ctcggcgtcg cgtggactca tcggctgtgc cgagccagaa agtccagggt gcccgccacg
     3181 atcacgtcgg cgtcattgtc gagcggtgcc acgtggtcgc tttcggtgag caccacttca
     3241 atgatctcgt ccgaggacac ctggcgcacc accacgtcct tggacgcgct gggcaccaca
     3301 ctgtcgagtt cggaccggta caacaggacc ggacagtgca ccaggtcgag ccgcgcccgc
     3361 acatcgctcc acagccgcat cagggcatgc acggccgcca gcggggtgcg ggggtaggcg
     3421 tgctcggtca catcggcctt gcggatgctg ctgccgatgc tgttggtggt gcggatcagg
     3481 tgcttggccc acggcaccag ggcgagctgc gggttgccgt gcagcgccgg gttgaccagc
     3541 accagcccgg ccacgtcgtc ggggtggtgc tccgcgagcc gcagcgccag tgctcccccc
     3601 atcgacaatc ccgcgacgaa caccatctgg catgaggccc gcaggtcgag gaaggcccgc
     3661 tcggccgccg cataccagtc gcggccatcg gtcacctcga gttcacgcca actggtgccg
     3721 tggcccggca gccgggggac cgacacccgg tatccgtccg cggcggtgcg ggtggcccac
     3781 tcgcgcaacg tccaggggct ggcggtgaag ccgtgcagaa acagcacgcc cacgtcgccg
     3841 gaccccgacg gcacgatcgg ctcggcgaac tcatgcacct gcatgcggcc catcgtacgg
     3901 tggcgcaccg acggtcgcgg cggcgctgga cggaccgcca ctgcccgtgc cccgataagg
     3961 tgtgcgcagt tcggttcgga ggaggtgcgg tgctgtacac gctgttcaag aacgtggcgt
     4021 ttcgcccggt gatcaagtac ctgttcggtg cccgggtggt gggcgagcgc aacattccct
     4081 ccgcaggcgg cgtgattctg gccagcaatc atctggacgc cggtgacacc gtcgtgctgc
     4141 ccgccatgat caagcgcaag gtcacgtttc cggccaaggc ggaactgttc aaaggcaacc
     4201 gaggactctt ctcgaagctc gtcgcctggt ttctgaccgc ggttggccag gtgccgctgg
     4261 accgctccgg gggtcgcgcc agcctggacg gactcggccc ggtgctgcag gtgctggccg
     4321 atggcggctg cgtgggcatc tatccagagg gcacccgttc gcccgacgga cggctgtaca
     4381 agggcaagac cggcgtggcg cgcatggcgc tggcggcgcg ggtgccgatc gtgccggtgg
     4441 cgatgcagaa cagcacgctg accaagaagt tcggcctgcc ctggatcgat cacccggtgg
     4501 tggtcgtggg cgagccgctc gatttcagcg agtacttcga ccgcggcgac gagccgcggg
     4561 tgctgcgctg ggtgaccgat caggtgatgg ccgccattca gagcctttcg gggcagacct
     4621 acgtcgaggc ctacggcacg tcggtcaaga gcgggtcgct gaccaaggcc gaggcggacg
     4681 cgcgcattct ggaacgtccg ggcagcaaca ccaggaaacc ggacgcgctg ccgcccaact
     4741 agccaggttc gtggacccat cgcgatgccg agagcacgca cctcgctaga tgagtgagcc
     4801 cgtcgaaacc cttggtcgca cgggtctcga cgaactcgac cagcgataga ccgtttctct
     4861 acctcgtgac caccgttact ccggtgcacc gccgggatct ctgtgtgagc cgagatccgg
     4921 ggcaagcccg ggatgacggg tacgccgctg ggcgagacgt tcgcagccga ggtctggaca
     4981 aagctcaacc agcggatcga tgcgtcgatc cgggcctaac ctgctcccgc acgtcacccc
     5041 aagggcctct cagccgggcc agactcctgg gcgcacccgc gtcccacgag cgggctcggt
     5101 tcggtggcgc cggaacgacc gcgtatcctg accggcgtgt cgacgcagat ccccactctt
     5161 gaacagttgc aggccctgag cccggttcag cagcccacct atgccgacac gcagcaggtc
     5221 gagcgggtga ctgcccagct gcgcacccgt ccgccgctgg tgttcgcggg ggagtgcgac
     5281 gacctgaggg cgaagatcgc cgatgtggcc gccggtcgcg cattcattct gcagggcggc
     5341 gactgcgccg agaccttcga caccggcacg gccctcgaca ttcgcggcaa gctgcgggtg
     5401 ctgctgtcga tggcggtggt gctcacctac gccgcgcagg tgcccgtggt caagatcggt
     5461 cggctcgccg gccagtacgc caagccgcgc agcaaggaca ccgagatccg tggcggtgtc
     5521 gaactgcccg cctaccgggg tgacgcggtc aacgggttcg acttcagcgc tgccgcacgc
     5581 gtccatgacc ccaaccggct gatggaggtc tacaaccact cggcggccac gctcaacctg
     5641 atccgggcgt tcgtcacggg tggtttcgcc gaccttcggg ccgtccattc gtggaatcag
     5701 gattttgtgc gcaactccgg cgtcgaggcc cgttacgagg cactggctga cgagatcgag
     5761 cgtgccctgg cgttcatggt ggcctgcggg gtcgacaagg aggcgtttcg caccgtcgac
     5821 ttctacagca gccacgaggc gttgctgctg ccctacgaac acgcgatgac acgcatcgac
     5881 tcgcgtaccg gcaccccgta caacacctcg gcgcacatgg tctggatcgg tgaacgcacc
     5941 cgccaggtcg acggtgccca cgtgcagttc gcccggcacc tgcgcaatcc gatcggcgtg
     6001 aagatcgggc cgtcgatgac cggcgacgag gtggtggcgc tggccgacgc gctcgacccg
     6061 cagcacgaac ccggacggct cacgctgatt tcacgcatgg gtgcgggcaa ggtgcgcacc
     6121 gcgctgccgc cgctggtgcg tgccgtcgag gcgaccggac gatcggtggc gtgggtgtgc
     6181 gatccgatgc acggcaacac cttcgagacc gccaacggct acaagacccg cgcgttcagc
     6241 caggtggtcg acgagatgaa cggcttcttc gacgtgcacg aagaactggg cacctggccc
     6301 ggcggcgtgc acatggagct gaccggcgac gacgtgaccg agtgcgtcgg cggcgtcgcg
     6361 gacgccctgg ccgaggccga cctgccgaac cgctacgaga ccgcctgcga cccccggctg
     6421 aaccgcaacc agtcgctcga actcgcgttt ctgatcgcgg aacggctcac cgacggccgc
     6481 atcaagcgga ccaacccggt gcaggagtac cgcgccgaag acttctgagc cgccgggcgg
     6541 cgcgtgcccg ggcggcgcgt gccgtccacc caaacgtcac cgactggcgg gcctgttcgt
     6601 cggcgtctgt tcatcgacgg ctcgcacggt ggccgggtgc gacgcaattg gctgatcttc
     6661 gcgaccgcgg cggccaccct gctggtgctg gcctacccgg tgaacaacaa cattcgctac
     6721 gaggtcggtg tgcaccgggt ctcgggctac tcgatcgccg agacgttctc acaccggccg
     6781 ctggcgtttc gactggtcag cgccgcccag tcgtggctgc ccgacctggc cagcgagtcg
     6841 ctggccgcac cggggtcgct ggccagggcc tggctgttcg aggcgggctt tcgactcgtc
     6901 gcagcactgg tggcggcagc ggcggcggtg ctgctcaccg tcggactgcg acgtcgtctg
     6961 ggctcggccg cctgggccta cgggctggcg gcgtacgcgg gcctgttctt caccgccccc
     7021 gccacgggcg aaccggactg gtgggcggcg gtggtggcgg tggcagccgt gggcaccgca
     7081 ctgctcgcgc gcgaatcgac cggcggatcg atggccggtg cactgctggc tgtggtcgcg
     7141 ttgctgaaga tcagctcgtt gccgatcgcg ttggcggccg tcatgctgat cgccgccttc
     7201 gac
//
LOCUS       DAOSUG010000091         7120 bp    DNA     linear   ENV 10-JUN-2024
DEFINITION  MAG TPA_asm: Micropruina sp. isolate AMR_MDS_2195
            NODE_5168_length_7120_cov_3.854636, whole genome shotgun sequence.
ACCESSION   DAOSUG010000091 DAOSUG010000000
VERSION     DAOSUG010000091.1
DBLINK      BioProject: PRJNA925477
            BioSample: SAMN32864262
            Sequence Read Archive: SRR4236673
KEYWORDS    WGS; ENV; Metagenome Assembled Genome; MAG; Third Party Data; TPA;
            TPA:assembly.
SOURCE      Micropruina sp. (wastewater metagenome)
  ORGANISM  Micropruina sp.
            Bacteria; Bacillati; Actinomycetota; Actinomycetes;
            Propionibacteriales; Nocardioidaceae; Micropruina.
REFERENCE   1  (bases 1 to 7120)
  AUTHORS   Abdulkadir,N., Saraiva,J.P., Zhang,J., Stolte,S., Gillor,O.,
            Harms,H. and Rocha,U.
  TITLE     Genome-centric analyses of 165 metagenomes show that mobile genetic
            elements are crucial for the transmission of antimicrobial
            resistance genes to pathogens in activated sludge and wastewater
  JOURNAL   Microbiol Spectr 12 (3), e0291823 (2024)
   PUBMED   38289113
REFERENCE   2  (bases 1 to 7120)
  AUTHORS   Abdulkadir,N.
  TITLE     Direct Submission
  JOURNAL   Submitted (24-MAR-2023) Environmental Microbiology, Helmholtz
            Center for Environmental Research -UFZ, Permoserstr 15, Leipzig
            04318, Germany
COMMENT     The annotation was added by the NCBI Prokaryotic Genome Annotation
            Pipeline (PGAP). Information about PGAP can be found here:
            https://www.ncbi.nlm.nih.gov/genome/annotation_prok/
            
            ##Genome-Assembly-Data-START##
            Assembly Method        :: SPAdes v. 3.11.1
            Genome Representation  :: Full
            Expected Final Version :: Yes
            Genome Coverage        :: Not Applicable
            Sequencing Technology  :: Illumina MiSeq
            ##Genome-Assembly-Data-END##
            
            ##Genome-Annotation-Data-START##
            Annotation Provider               :: NCBI
            Annotation Date                   :: 05/04/2023 04:57:10
            Annotation Pipeline               :: NCBI Prokaryotic Genome
                                                 Annotation Pipeline (PGAP)
            Annotation Method                 :: Best-placed reference protein
                                                 set; GeneMarkS-2+
            Annotation Software revision      :: 6.5
            Features Annotated                :: Gene; CDS; rRNA; tRNA; ncRNA
            Genes (total)                     :: 2,776
            CDSs (total)                      :: 2,750
            Genes (coding)                    :: 2,714
            CDSs (with protein)               :: 2,714
            Genes (RNA)                       :: 26
            tRNAs                             :: 24
            ncRNAs                            :: 2
            Pseudo Genes (total)              :: 36
            CDSs (without protein)            :: 36
            Pseudo Genes (ambiguous residues) :: 0 of 36
            Pseudo Genes (frameshifted)       :: 2 of 36
            Pseudo Genes (incomplete)         :: 32 of 36
            Pseudo Genes (internal stop)      :: 2 of 36
            ##Genome-Annotation-Data-END##
FEATURES             Location/Qualifiers
     source          1..7120
                     /organism="Micropruina sp."
                     /mol_type="genomic DNA"
                     /submitter_seqid="NODE_5168_length_7120_cov_3.854636"
                     /isolate="AMR_MDS_2195"
                     /isolation_source="Activated sludge of municipal
                     wastewater treatment plants"
                     /db_xref="taxon:2737536"
                     /environmental_sample
                     /geo_loc_name="Canada"
                     /lat_lon="49.25 N 123.1 W"
                     /collection_date="2015-06-16"
                     /metagenome_source="wastewater metagenome"
                     /note="metagenomic"
     gene            complement(<1..543)
                     /locus_tag="PLF56_04445"
     CDS             complement(<1..543)
                     /locus_tag="PLF56_04445"
                     /inference="COORDINATES: ab initio
                     prediction:GeneMarkS-2+"
                     /note="Derived by automated computational analysis using
                     gene prediction method: GeneMarkS-2+."
                     /codon_start=1
                     /transl_table=11
                     /product="hypothetical protein"
                     /protein_id="HOQ52843.1"
                     /translation="MFVHRVVERTVSLLLATGVGLVTLLATPAPAMAAAIGALAGAGP
                     RPQATRLKFDAGDRVKAQVDVGSGNLYVTVRGIALQGVSAQVEIGAFYNSAAAASSPT
                     PRLGKGWGLDYTGDVRVAQQSDNSVTYYGPGGLVGNFPLVSGSTTTYTSAKGFKSDLV
                     KSSSGWTLTERTSKVKRTFDT"
     gene            complement(1653..2144)
                     /locus_tag="PLF56_04450"
     CDS             complement(1653..2144)
                     /locus_tag="PLF56_04450"
                     /inference="COORDINATES: protein motif:HMM:NF033179.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="TnsA-like heteromeric transposase endonuclease
                     subunit"
                     /protein_id="HOQ52844.1"
                     /translation="MAEIGAQDVVRGLPVCSFPTYMGQRNYPGLLWMATTQSLVGYES
                     LLERDRLWLADFDPTVSVVLTQPFWVSGRDGSVLRRHVPGFLLETTRGYVVVDVKPAE
                     LLDDAVVAAVLNWTRRLCEAKGWTYEVRSGADPMLLRTSVFWRRHGEVMRQRRKPSAG
                     LPT"
     gene            complement(2376..3098)
                     /locus_tag="PLF56_04455"
     CDS             complement(2376..3098)
                     /locus_tag="PLF56_04455"
                     /inference="COORDINATES: ab initio
                     prediction:GeneMarkS-2+"
                     /note="Derived by automated computational analysis using
                     gene prediction method: GeneMarkS-2+."
                     /codon_start=1
                     /transl_table=11
                     /product="hypothetical protein"
                     /protein_id="HOQ52845.1"
                     /translation="MTDAPLFLRLQTGDREEAVPVDTTGGLIRFAVGAVGERSSIWRV
                     WANKNKSDVYIAARELARYQKYSLHESGDYRYAWTSEHQDTTPATRVIDRWQRPDPNE
                     GGWSRGISVYVRAEDVVHIHQDRTKESDVAWLDGPPAGKALGIHIFLLTPDIGWTELK
                     GVQPLHIIGLADRRAVLILSSVVDVDPEQNRRITHWQRTALADAPDKLRLSPAAARSN
                     LRMAVFGYDDQGHRNIWDTALT"
     gene            complement(3095..3613)
                     /locus_tag="PLF56_04460"
     CDS             complement(3095..3613)
                     /locus_tag="PLF56_04460"
                     /inference="COORDINATES: ab initio
                     prediction:GeneMarkS-2+"
                     /note="Derived by automated computational analysis using
                     gene prediction method: GeneMarkS-2+."
                     /codon_start=1
                     /transl_table=11
                     /product="hypothetical protein"
                     /protein_id="HOQ52846.1"
                     /translation="MTRYRWTGISAPFGGLQWERKDDDREIARRVLNLLSDRRMLWKD
                     FSLEIEEHCVTSANKIRDQLGEQMNNPEIGTDLARRLQLLQRLFRDFVDHIGPASSDR
                     HRHWQPLGTDPLSMALGRLRGLVGIQIGELAADYNLDVSKELATIVPDQAGWFFEPFD
                     STEDPDTQESRL"
     gene            complement(3610..4002)
                     /locus_tag="PLF56_04465"
     CDS             complement(3610..4002)
                     /locus_tag="PLF56_04465"
                     /inference="COORDINATES: protein motif:HMM:NF040041.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="DUF6262 family protein"
                     /protein_id="HOQ52847.1"
                     /translation="MLADNSHHLLAAAKRRHNDAQQRAREAIRLAAARGEAVTPTAVA
                     TRAGVSRAFLYANPELIEAIHELRDRNRGAHATAGHKSTSETSTSLQRRLEAVTQRNK
                     ELRSENHDLRQRLEIAYGQLRAQDQGAK"
     gene            complement(3992..6064)
                     /locus_tag="PLF56_04470"
     CDS             complement(3992..6064)
                     /locus_tag="PLF56_04470"
                     /inference="COORDINATES: protein motif:HMM:NF012798.2"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_function: GO:0003677 - DNA binding [Evidence IEA];
                     GO_process: GO:0006310 - DNA recombination [Evidence IEA];
                     GO_process: GO:0015074 - DNA integration [Evidence IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="tyrosine-type recombinase/integrase"
                     /protein_id="HOQ52848.1"
                     /translation="MAHADRWYRARRRGVVFEAWLQNCPPRCTPQACLVPGCLFGRHR
                     SQLCLNHHATWIAAAGHGSAQNWAGIVQAESTDQPGCLVPGCGLWASFRDGFCVSHQG
                     RFNAFRRRTGTADRDAFLAAVAVAGRPRIDLTGLAPQLKLEVQYIVQCFLDSGPRRVN
                     LTRWNAVVRALHARGATSLLEHSAVEWTSSLRLSRRNPDGALFINWGHDQVDRLVHGT
                     GWDREYSLDTWHLDRLGHATTGVARITFSKIPQPWLRGLAKQWARHRLSIGVGPAGVR
                     RGIGVLGQFSTYITNLKDPPRDTGDLTRVMVQRWCENLALDHPEPRSRLQLISALSTF
                     LRDVHRYGWAPELASTTVVFSEDFPRLHGTGPGRALSEFVVAQIESDEALARLTHPDH
                     QLMTRIMIRCGLRAGDCRLLAFDCLVQDGEGHPYLQYLNHKMKRTAFMPLDDDLASRI
                     RDQQCTVLERFDQPRPGLRLFPGHASNPHGTRAYPASGHRAAFQAWLADLKITDELGQ
                     PVWITPHQLRHTFGTRMINRDVPQHIVQQLMDHTSADMTAHYARLNDKTIRDAWAKAQ
                     LINAHGQPVELDEDHPLADAAWARRGLDRAKQTLPNGYCGMPLNSPCEHANPCLTCPM
                     FITNADFLPQHETQLKHTLELIDISREKGHLRVVEKNQEIVDNLQRIITACQTRPTTI
                     DQKAADAC"
     gene            complement(6250..7023)
                     /locus_tag="PLF56_04475"
     CDS             complement(6250..7023)
                     /locus_tag="PLF56_04475"
                     /inference="COORDINATES: protein motif:HMM:NF012798.2"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_function: GO:0003677 - DNA binding [Evidence IEA];
                     GO_process: GO:0006310 - DNA recombination [Evidence IEA];
                     GO_process: GO:0015074 - DNA integration [Evidence IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="tyrosine-type recombinase/integrase"
                     /protein_id="HOQ52849.1"
                     /translation="MSAFYGFYRRLDPVITLQLTRWDRGFSSAGYQPFLAHTRREHAR
                     QEIRVRGVLRSAPEVVEAEAYQALLDGCTRLRDRFLLQLLRESGMRIGEALGLRHEDL
                     SIARREVAVNPRLNANGARVKRWKPRTIPAPAALFSSYADYLDEEYGDLNSDFVFVNL
                     WAAPLGHAMAYPAARDVLLRLRRRTGLSSFTFHHLRHTYATELIRKGTDWAIVAHLLG
                     HASVQTTLGIYGHLTVEDARRALIAAGWLIEPVQALEVP"
BASE COUNT         1221 a         2105 c         2458 g         1336 t
ORIGIN      
        1 ggtgtcgaac gtgcgcttca ccttcgaggt gcgctccgtc aacgtccaac cactggagga
       61 cttcaccagg tcggacttga accccttcgc cgaggtgtag gtggtggtgg agcccgacac
      121 cagcgggaaa ttgccgacca gtccgcccgg cccgtagtag gtgaccgagt tgtccgactg
      181 ctgcgccacc cggacatcac cggtgtagtc caacccccac cccttcccca accgtggtgt
      241 cgggctggat gcggccgcag ccgagttgta gaaagcgccg atctcaacct gcgcagacac
      301 cccttgcaac gcgatcccgc ggaccgtcac atacagattg cccgagccga catccacctg
      361 ggccttcacc cgatccccgg catcgaactt caaccgggtc gcctgcggcc gcggcccagc
      421 cccagccaaa gcaccaatcg cggcagccat cgccggcgca ggagtcgcca acaacgttac
      481 caaacccaca cccgtggcca agagtaacga gacagtgcgc tcgacaacac ggtgaacaaa
      541 catgggggtg gcctttcaca aggcattatt gatcgaaacg cgaaaaaagt cttcgtcggg
      601 gtggggtatt tgctggttga gtcccttgaa cggatttttt tgcttactga atgagtccaa
      661 cgaccgcaag tatttggggt gcactgtcga tgggtccaca cccttccggg tgcatcgact
      721 agacttctcg cttagctcag cgtggctgta accgacttgt aacgagatcc agctggatta
      781 tttgtcaaga gctattgata gacactcttt ggagatcaag gacgcagcca tccgtaaaca
      841 cccttgagct gcatgtgctg gcgatcatta gggcagccga accgtaccgt ggcggtcggg
      901 agggacccgg atgatggtgc ccagcggagt ggccccctcg gctgcgccaa cagtcagtag
      961 atgacggtgt gccgcgtgga acaggtcggc atgcctcaac gggcgtagcg ggcgaagagc
     1021 gttgtagtcg ggagtggtgg cgtcgagccg tagcgcttcg ggcccgtaag cgatgttgcc
     1081 gccggtgaaa cctctcccag aactgttggg tgagtgtgag caggtccgtg tcggtggggg
     1141 tatgcgacgt ccaatgtgga cggcttcggt gaggaggtcg gtatagcggc caatgagccg
     1201 gagttggtgg tggtcaatgg gtggcggagg gtgttggagc cgggtgatca ggttgtctag
     1261 agcgaggtgg atcacgggcc aggctccgcg agcctgcgca tatcgcaggt agctgccgat
     1321 ccggttggcg tgcgtgggcg gcaggcgggg ttggccggtg atggtggccc actgggcgtg
     1381 gttgccgatc tttcaaggtg aagcgtactg gctgaaccga tgaaagatcg aggctacggc
     1441 aggctagggc ccccgttgcg ttgggtcgtg gacctgcccc gccccctcac cggcgcttca
     1501 gtgcttcagg acgtggcatg acggctggca gcgttgagtt caaggtcggc gaccgggtct
     1561 ggtacgaccc gcccacaaca tcgacatcga ggccggtcgg gcccgattga cctcgatgcc
     1621 tgaggcggct tccacgtcgt cgatcgtcat gcctacgttg gcaatcctgc cgatggcttc
     1681 ctcctctgcc gcatcacctc gccgtgccgc cgccagaaaa cggatgttcg aaggagcatt
     1741 gggtcagcac ctgacctcac ctcgtaggtc catcccttgg cctcgcacaa gcgacgcgtc
     1801 cagttcagga cggccgcaac gacggcatca tcgagcaact cggccggctt gacgtcaaca
     1861 acgacgtatc cacgggtcgt ctccaggagg aagcctggga cgtgtcgccg aagcaccgat
     1921 ccgtctcggc cagataccca gaacggctgg gtcaacacca ccgacaccgt cggatcgaag
     1981 tcggccagcc agagccgatc ccgctcgaga agactctcgt acccgaccag cgactgggtc
     2041 gtcgccatcc aaagcaggcc cgggtagttg cgttgaccca tgtacgtcgg gaagctgcag
     2101 accggcagtc cccgcacaac atcctgggca ccgatctcag ccaagctggt ccactcgcaa
     2161 cggtccagcg cagctaggaa ccggagctct gcgtttcgca gcgcccaccg ctcatggccg
     2221 cagcatcaca cgccgacaac atgatgggag cccgacacgc cgccgacaag ataatgagtt
     2281 atctggagtt gatgtccata acagtgcttc tgactaggca gtttccgtga cggacagtgt
     2341 cattgatgca gtcggctgat gtcagtggcc gagcattaag taagtgccgt atcccagatg
     2401 ttgcgatggc cctggtcgtc gtagccgaac acggccatcc gcaggttgga gcgggctgcg
     2461 gccgggctaa gacgcagttt gtcgggtgcg tccgcgagtg ccgttcgttg ccagtgtgtg
     2521 attcgcctgt tctgctcagg gtcgacgtcg acgacactgc tcaggatcag cacggctcga
     2581 cggtcagcta ggccaatgat gtggagtggc tgaactcctt tcagttccgt ccagccgata
     2641 tcaggggtca acaggaagat gtggatgcct agcgcttttc ctgcgggggg cccgtcgagc
     2701 catgcaacgt cggattcttt ggtgcggtcc tggtggatgt ggacgacgtc ctcggctctg
     2761 acgtagaccg agatgcctcg gctccaacca ccttcgttgg gatcggggcg ttgccagcgg
     2821 tcgatgactc gcgtcgcggg tgtggtgtct tgatgttcag acgtccaggc gtagcggtag
     2881 tcgccactct cgtgcagcga gtacttctga taccgcgcga gctctcgtgc ggcgatgtag
     2941 acgtcgctct tgttcttgtt ggcccaaacc ctccagatgc tggagcgttc gccgacggca
     3001 cccacagcga atcggatgag cccgcccgtg gtgtcaacag gcactgcttc ctcacggtct
     3061 cctgtttgga ggcgaaggaa caggggagcg tcggtcacag tctgctctcc tgtgtgtcgg
     3121 ggtcctccgt agagtcgaac ggctcaaaga accatccggc ctggtcgggc acaatagtgg
     3181 ccagctcttt ggacacgtcg aggttgtagt cggcggccag ttcgccgatc tggattccaa
     3241 cgaggccccg caggcgtccg agcgccatcg agaggggatc ggtgcccagc ggctgccaat
     3301 gtcggtgccg gtcgctgctg gcgggcccga tgtggtcgac gaagtcgcgg aatagtcgct
     3361 gcagcaactg caggcggcgg gcgaggtcgg tgccgatctc gggattgttc atctgctcgc
     3421 ccagctgatc acggatcttg ttggccgagg tgacgcagtg ttcctcgatc tccagagaga
     3481 agtccttcca gagcattcgt cggtcgctca gcaggttaag gactcgtcgg gcgatctcgc
     3541 ggtcatcgtc tttgcgttcc cattggaggc cgccgaaggg tgctgagatc ccggtccatc
     3601 ggtagcgggt catttggccc cttggtcctg cgctcgaagt tgaccgtagg cgatctcgag
     3661 ccgctgtcgc agatcgtgat tctcgctgcg tagctctttg ttgcgctggg tgactgcttc
     3721 gagccgacgc tgcagggacg tggacgtttc gcttgtgctc ttgtggcctg cagttgcgtg
     3781 tgcaccgcgg ttccggtcac gcagttcgtg gatggcttcg atgagctcgg ggttggcgta
     3841 gaggaaggca cgggagacgc cggcgcgagt ggccacggcc gtgggggtga cggcttcgcc
     3901 ccgggccgcg gcgaggcgaa tagcttctcg tgcgcgttgt tgggcgtcgt tgtgtcgtcg
     3961 cttcgcggcg gccaggaggt ggtggctgtt gtcagcaagc atcggcggcc ttctggtcga
     4021 ttgtggtggg ccgggtctga caggcggtga tgatgcgctg caggttgtcg acgatctcct
     4081 ggttcttttc cacgacgcgt agatggccct tttcgcggga gatgtcgatg agttcgaggg
     4141 tgtgcttgag ctgggtctcg tgttggggga ggaagtctgc gttggtgatg aacatggggc
     4201 aggtcaggca ggggttggcg tgctcgcagg ggctgttgag gggcatgccg cagtagccgt
     4261 tgggcagggt ctgcttggcg cggtcgaggc cgcggcgggc ccaggcggcg tcggcgagtg
     4321 ggtggtcctc gtcgagctcc accggctggc cgtgggcgtt gatgagttga gccttggccc
     4381 aggcgtcgcg gatggtcttg tcgttgagcc gggcgtagtg ggcggtcatg tcggccgagg
     4441 tgtggtccat gagctgctgc acgatgtgtt gcggaacgtc gcggttgatc attcgggtgc
     4501 cgaaggtgtg tcgaagctgg tgtggcgtga tccaaaccgg ctgaccaagc tcgtcggtga
     4561 tcttgaggtc agcaagccag gcttgaaatg cggcccggtg gccggaggcg ggataagccc
     4621 tggttccgtg aggattggac gcgtgcccgg ggaatagccg tagtcccggg cggggttggt
     4681 cgaagcgttc caggacggtg cactgctggt cacggatgcg gctggcgagg tcgtcgtcga
     4741 gcggcatgaa cgcggtgcgc ttcatcttgt ggttcaggta ctgcaggtaa gggtggccct
     4801 cgccgtcctg gacgaggcag tcaaacgcca gcaggcggca gtcgcccgcc ctgagtccgc
     4861 agcggatcat gatccgcgtc atcaactggt gatcaggatg ggtgagccga gcgagtgcct
     4921 cgtcggactc gatctgggcc acgacgaact cgctgagcgc gcggccgggt ccggtgccgt
     4981 gcaggcgggg gaagtcctcg ctgaacacca ccgtggtaga cgccagctcg ggtgcccagc
     5041 cgtagcggtg gacgtcccgc aggaacgtcg acagagcgct gatcagttgc aggcgcgacc
     5101 tcggttcagg atggtctagg gctaggttct cgcaccagcg ctggaccatg actcgagtca
     5161 ggtcgcccgt gtcacgagga gggtctttga ggttggtgat gtaagtcgag aactggccta
     5221 ggacaccgat acctcttcgg acaccggccg ggccgacgcc gatgctgagc cgatggcggg
     5281 cccactgctt tgccaagccc cgcagccatg gttgcgggat cttggagaag gtgatgcggg
     5341 cgacgccggt ggttgcatgc cccagccgat cgagatgcca ggtgtcgagg gagtactcgc
     5401 ggtcccagcc ggtgccgtgc acgagccggt cgacctggtc atggccccag ttgatgaaca
     5461 gggcaccgtc ggggttgcgg cggctcaatc gcaacgaact ggtccactcc acagcgctgt
     5521 gctcgaggag gctggtggcg cctctggcgt ggagggcgcg aacaacggca ttccatcgcg
     5581 tgaggttgac ccgccgtggg ccggagtcga ggaagcattg gacgatgtac tggacctcga
     5641 gcttcagctg aggagctaac ccggtgaggt cgatccgggg tctgccggcg accgcgaccg
     5701 cggcgaggaa cgcgtctcgg tccgctgtgc cggttcggcg acggaaggcg ttgaaccggc
     5761 cctggtggga gacacagaac ccgtcgcgga acgacgccca caacccacag ccaggcacca
     5821 gacagcctgg ctgatcggtc gactcagcct gcacgattcc cgcccagttc tgcgcgctgc
     5881 catggccggc ggccgcgatc cacgtggcgt ggtggttaag gcacaactgg ctgcggtgcc
     5941 ggccgaagag gcatccgggc accagacatg cctgcggcgt gcagcgcggc gggcagttct
     6001 gcagccatgc ctcgaagacg acaccacgcc gccgggcccg gtaccaacgg tcggcatggg
     6061 ccacgcacag accgcggccc gcggtcgcca cacctgagca atccgggacc cgacacgagc
     6121 cgagatcgac caacggatga caggcctccg gaatgagaac gtcaccgcgg tgttcggggc
     6181 gaaccgcagc catcaacgcg gccaaaggtg actcgcccag cggcccagca ggggcagatg
     6241 tcagcgtcgt catggcacct ccagggcttg aacgggttcg atcagccagc cggcggcgat
     6301 cagtgcccga cgggcgtctt ccacggtcag gtgtccgtag atcccgagcg tcgtctgaac
     6361 actggcgtgg cccagcaggt gcgccacgat cgcccagtcg gtgcccttgc ggatcagttc
     6421 ggtggcgtag gtgtgccgca ggtgatggaa cgtgaagctg gacagtccgg tgcggcggcg
     6481 cagccgcagc agcacgtccc gtgcggccgg gtaggccatc gcgtgaccga gtggcgcggc
     6541 ccacaggttg acgaagacga agtcggagtt gaggtcgccg tattcctcgt cgaggtagtc
     6601 ggcgtaactg ctgaacagcg cggccggtgc ggggatagtt ctgggcttcc agcgcttcac
     6661 ccgagccccg ttcgcgttga gccgagggtt gaccgcaacc tcacggcgtg cgatcgagag
     6721 gtcctcgtgg cgcaatccaa gcgcttctcc gattcgcatg cccgattcgc ggagcagttg
     6781 cagcaggaac cggtcgcgca gccgcgtgca cccgtccagc agtgcctggt atgcctcggc
     6841 ctcgacgacc tccggggcgc ttcgcaggac gccacggacc cggatctcct gacgtgcgtg
     6901 ctcgcgtcga gtgtgggcca gaaaaggctg gtaaccggca gacgagaagc cccggtccca
     6961 ccgggtcagc tgcagcgtga tcaccgggtc gaggcggcgg tagaagccat agaaggctga
     7021 cactgcagcg atcttgcgct gcaaagtccg ctcacccagg gaaggctcaa cgttgggcaa
     7081 gaccccgacc cgcccttgtc tagccggact cggaagccgc
//
LOCUS       DAOSUG010000092         7089 bp    DNA     linear   ENV 10-JUN-2024
DEFINITION  MAG TPA_asm: Micropruina sp. isolate AMR_MDS_2195
            NODE_5183_length_7089_cov_4.180125, whole genome shotgun sequence.
ACCESSION   DAOSUG010000092 DAOSUG010000000
VERSION     DAOSUG010000092.1
DBLINK      BioProject: PRJNA925477
            BioSample: SAMN32864262
            Sequence Read Archive: SRR4236673
KEYWORDS    WGS; ENV; Metagenome Assembled Genome; MAG; Third Party Data; TPA;
            TPA:assembly.
SOURCE      Micropruina sp. (wastewater metagenome)
  ORGANISM  Micropruina sp.
            Bacteria; Bacillati; Actinomycetota; Actinomycetes;
            Propionibacteriales; Nocardioidaceae; Micropruina.
REFERENCE   1  (bases 1 to 7089)
  AUTHORS   Abdulkadir,N., Saraiva,J.P., Zhang,J., Stolte,S., Gillor,O.,
            Harms,H. and Rocha,U.
  TITLE     Genome-centric analyses of 165 metagenomes show that mobile genetic
            elements are crucial for the transmission of antimicrobial
            resistance genes to pathogens in activated sludge and wastewater
  JOURNAL   Microbiol Spectr 12 (3), e0291823 (2024)
   PUBMED   38289113
REFERENCE   2  (bases 1 to 7089)
  AUTHORS   Abdulkadir,N.
  TITLE     Direct Submission
  JOURNAL   Submitted (24-MAR-2023) Environmental Microbiology, Helmholtz
            Center for Environmental Research -UFZ, Permoserstr 15, Leipzig
            04318, Germany
COMMENT     The annotation was added by the NCBI Prokaryotic Genome Annotation
            Pipeline (PGAP). Information about PGAP can be found here:
            https://www.ncbi.nlm.nih.gov/genome/annotation_prok/
            
            ##Genome-Assembly-Data-START##
            Assembly Method        :: SPAdes v. 3.11.1
            Genome Representation  :: Full
            Expected Final Version :: Yes
            Genome Coverage        :: Not Applicable
            Sequencing Technology  :: Illumina MiSeq
            ##Genome-Assembly-Data-END##
            
            ##Genome-Annotation-Data-START##
            Annotation Provider               :: NCBI
            Annotation Date                   :: 05/04/2023 04:57:10
            Annotation Pipeline               :: NCBI Prokaryotic Genome
                                                 Annotation Pipeline (PGAP)
            Annotation Method                 :: Best-placed reference protein
                                                 set; GeneMarkS-2+
            Annotation Software revision      :: 6.5
            Features Annotated                :: Gene; CDS; rRNA; tRNA; ncRNA
            Genes (total)                     :: 2,776
            CDSs (total)                      :: 2,750
            Genes (coding)                    :: 2,714
            CDSs (with protein)               :: 2,714
            Genes (RNA)                       :: 26
            tRNAs                             :: 24
            ncRNAs                            :: 2
            Pseudo Genes (total)              :: 36
            CDSs (without protein)            :: 36
            Pseudo Genes (ambiguous residues) :: 0 of 36
            Pseudo Genes (frameshifted)       :: 2 of 36
            Pseudo Genes (incomplete)         :: 32 of 36
            Pseudo Genes (internal stop)      :: 2 of 36
            ##Genome-Annotation-Data-END##
FEATURES             Location/Qualifiers
     source          1..7089
                     /organism="Micropruina sp."
                     /mol_type="genomic DNA"
                     /submitter_seqid="NODE_5183_length_7089_cov_4.180125"
                     /isolate="AMR_MDS_2195"
                     /isolation_source="Activated sludge of municipal
                     wastewater treatment plants"
                     /db_xref="taxon:2737536"
                     /environmental_sample
                     /geo_loc_name="Canada"
                     /lat_lon="49.25 N 123.1 W"
                     /collection_date="2015-06-16"
                     /metagenome_source="wastewater metagenome"
                     /note="metagenomic"
     gene            <1..693
                     /locus_tag="PLF56_04480"
     CDS             <1..693
                     /locus_tag="PLF56_04480"
                     /EC_number="2.5.1.74"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_015071322.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_component: GO:0016020 - membrane [Evidence IEA];
                     GO_function: GO:0046428 - 1,4-dihydroxy-2-naphthoate
                     octaprenyltransferase activity [Evidence IEA];
                     GO_process: GO:0009234 - menaquinone biosynthetic process
                     [Evidence IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="1,4-dihydroxy-2-naphthoate
                     polyprenyltransferase"
                     /protein_id="HOQ52850.1"
                     /translation="DGVRGTDADRVGPMRLVGSGAAEAPRVKAAAFGCFAVAGLAGLG
                     IVLLTQLWWLLAVGAAAIAAAWLYTGGSRPYGYLGLGEVFVFVFFGLVAVSGTVLVQV
                     GSVPAAAWAAAVAVGALACAILVANNLRDLRGDAAVGKRTLATRLGDRGTRWFFTILV
                     LVALGSIVLVAAWSTPWALLGLLMVVFVVGPVREVLGGGSARSLIGVLKLVGVAELVA
                     AAGLAVGLVIGA"
     gene            686..1666
                     /locus_tag="PLF56_04485"
     CDS             686..1666
                     /locus_tag="PLF56_04485"
                     /EC_number="4.2.1.113"
                     /inference="COORDINATES: protein motif:HMM:NF002782.0"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="o-succinylbenzoate synthase"
                     /protein_id="HOQ52851.1"
                     /translation="MPEGSPIERALTYDVGLTTRFRGITRRSGVLLQGSAGWGEWSPF
                     PEYRRPEIDAWWAAAVESATLPWPAPVRDSVPVNSIVPAVEPEVAGRFALAGGCRTAK
                     VKVAEPSQTLADDLARVEAVRDALGPHGRVRVDANGAWGVDEALAAIRQLARFDLEYV
                     EQPCATVEELADLRVRLARVGVQVRIAADESIRRAEDPGRVVALAAADVAVLKVQPLG
                     GVRRCLHLAEQLGLPVVVSSAIETSVGIAAGVALAAALPQLPFACGLNTLALLSTDVA
                     DDPLVVRDGQLDVRRVAPGGVGWQASDEVDRWWQQRRRTVEAGAGQVAGR"
     gene            1663..3297
                     /gene="menD"
                     /locus_tag="PLF56_04490"
     CDS             1663..3297
                     /gene="menD"
                     /locus_tag="PLF56_04490"
                     /EC_number="2.2.1.9"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_011754002.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_function: GO:0070204 -
                     2-succinyl-5-enolpyruvyl-6-hydroxy-3-cyclohexene-1-
                     carboxylic-acid synthase activity [Evidence IEA];
                     GO_process: GO:0009234 - menaquinone biosynthetic process
                     [Evidence IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="2-succinyl-5-enolpyruvyl-6-hydroxy-3-
                     cyclohexene-1-carboxylic-acid synthase"
                     /protein_id="HOQ52852.1"
                     /translation="MNDGTIRPGGGATGSSACAATIVAQLVVQGVREVVLAPGSRSAP
                     LALALARAEARDEVRLHVRYDERSAGFLALGLAKASGVPVAVVTTSGTAVGNLLPAVM
                     EAHHAGVPLLVVSADRPAAMVGFGANQTTDQVRLFEPFVRWSARVSSAAPSESWGAQA
                     ARAWLIATGVLSRHPGPVHLNVELAEPLVGAAPEPQVQAVSAAAAPGAEPLVLPSGPR
                     TLLVCGDASVEVGTSVAELAVAAPLPLVAEPSSNARAAALHTGRLLLGGPLAQRVQRV
                     VVFGHPTLSRPVNQLLARPDVELIVVSPTSDWVDPGWRASRVASAVGIERDDSSWAEE
                     WAAADVRLIAALNELVGAQQTFTGPALAASVAASVSPGDVLVLGSSQPIRDADLAPLG
                     RQVVYANRGLAGIDGTVSTAVGIALATGRPATLLCGDLTFLHDVNGLAGMGERRPDLR
                     IVVADDSGGSIFATLEYGAPEFADAFERVFATPSGVDVAGIARAHGLPVVQVSTASEL
                     ATALKAPIRGVELIVARIDRVGRRALDQAINALGRR"
     gene            complement(3640..5346)
                     /locus_tag="PLF56_04495"
     CDS             complement(3640..5346)
                     /locus_tag="PLF56_04495"
                     /inference="COORDINATES: protein motif:HMM:TIGR03491.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="TM0106 family RecB-like putative nuclease"
                     /protein_id="HOQ52853.1"
                     /translation="MSAVQPARQAQPFALDAYAARSCPVKTHNAFHPGLVMPSTADEG
                     VREVFHGGVNFEAEVLSRLLGGFTGSVLDCRELRELPTSEQERITLAAMAAGVQVLIA
                     PLLPRDLVRHRSGRPDLLLRADDGGYHPAEIKCHRVSDVRREPAVLTWSSLTDPLSRH
                     DLPGRRFRYNWRLNDLLQLAHYHRMLQAVGRASATRLAAVVGTDELPELGRVLSWVDL
                     TLPVVPPSPRERTDPYDVELVSSLERYDAEHAFRVELAEAASALAAGDPPLLQPIANR
                     ECGWCQWWDVCRPRLDDDDLSLRISKSPLDVHEIQVLRDLGVATVTELAATDLEALLP
                     SYLARVTHRVGAEDRLRLAWRRSVLMARGVDFDRVTQGPIDVPGATVEIDLDIETSVG
                     DRVYLWGFLVSDSRDGSHYYRHFSAFDELTDESEAALAEAAMQWLRSLVEGVDARVYH
                     YSDYEVVRLERLAVRSGSPVLEWARGWGRGHFVDLFTSIRTHFFGTQGLGLKVVASKA
                     AGFAWRDATPGGLNSQAWFDEAVAGETAAARAAARQRVLDYNEDDVEATWHVRRWLRG
                     LT"
     gene            complement(5356..6060)
                     /locus_tag="PLF56_04500"
     CDS             complement(5356..6060)
                     /locus_tag="PLF56_04500"
                     /EC_number="2.1.1.163"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_004809135.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_function: GO:0008168 - methyltransferase activity
                     [Evidence IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="demethylmenaquinone methyltransferase"
                     /protein_id="HOQ52854.1"
                     /translation="MVETGRARATLAKRRADVAAMFDGVAARYDLVNDLLTGGLDRGW
                     RRAVLAAVAPRPGQRILDLAAGTGTSSRPFADAGALVVAADLSLGMLQVGAERQPGLT
                     FVNADALSLPFADAAFDAVTISFGLRNVEDTAAALAELRRVTRPGGRIVICEFSTPTF
                     PPLRWAYQQVALRALPLVARLGASNAVAYDYLAESILAWPAQRRLAELMTAAGWADVQ
                     WKNLTGGIVALHRARA"
     gene            6200..6862
                     /locus_tag="PLF56_04505"
     CDS             6200..6862
                     /locus_tag="PLF56_04505"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_013160433.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_function: GO:0005524 - ATP binding [Evidence IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="ABC transporter ATP-binding protein"
                     /protein_id="HOQ52855.1"
                     /translation="MAKRSTVLKVAGLSKSFGPVVIVDNFNLEVREGEAIALTGRNGA
                     GKSTILRCIVGADQPDAGTVEVSGIRMSETNPEVRRDVATVIDDLDFFPDLSVVEHLD
                     LLARAHGIDEPEAAVDEVLHEVQLVPQSGQLPGTLSSGQRRRLALATAFIRPRKLLVL
                     DEPEQRLDVEGVEWLAARLQAERARGLAIVMASHEPRLVQSVATRIVRIGDGVSADEV
                     VG"
     gene            6859..>7089
                     /locus_tag="PLF56_04510"
     CDS             6859..>7089
                     /locus_tag="PLF56_04510"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_013160434.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="ABC transporter permease"
                     /protein_id="HOQ52856.1"
                     /translation="MSKKSRKLIRSSQVAPATAPTMEPAAEPTAPQQNFWFLPDTAPH
                     PVDERDLHDLIKQWRHGRATRSVWDAIQDAYVA"
BASE COUNT          947 a         2449 c         2629 g         1064 t
ORIGIN      
        1 gacggggtgc gcggcaccga cgccgaccgg gtcgggccga tgcggttggt cggctcgggc
       61 gccgcggagg cgccccgggt gaaggcggcc gcgttcggct gcttcgcggt ggcggggttg
      121 gccgggctcg gcatcgtgct gctcacccag ctgtggtggc tgctcgccgt cggcgcggcg
      181 gcgatcgcag cggcctggct ctacaccggt ggctcgcgtc cctacggcta cctgggcctg
      241 ggcgaggtgt tcgtgttcgt gttcttcgga ctggtcgcgg tctcgggcac ggtgctggtg
      301 caggtgggct ccgttcctgc tgccgcctgg gcggccgcgg tcgccgtcgg tgctctggcc
      361 tgcgcgatcc tggtggccaa caacctgcgc gacctgcgcg gcgacgccgc ggtcggcaaa
      421 cggacgctgg ccacccggct gggcgatcgg ggcacccggt ggttcttcac catcctggtg
      481 ctggtggcgc tgggctcgat cgtgctggtc gccgcctgga gcacgccgtg ggcgctgctg
      541 gggttgttga tggtggtgtt cgtggtgggt ccggtgcgcg aggtgctggg cggcggttcc
      601 gcacgctccc tgatcggcgt gctgaagctc gtgggggtgg ccgaacttgt ggctgccgcg
      661 gggctcgcgg tggggctggt gatcggtgcc tgaggggtcg ccgatcgagc gggcgctcac
      721 ctacgacgtg gggctgacca cccgattccg cggcatcacc cggcgttcgg gggtgttgtt
      781 gcagggctcg gccggatggg gggagtggag cccgttcccc gagtaccggc gacccgagat
      841 cgatgcctgg tgggccgccg cggtcgagtc ggcgacgctg ccgtggcccg ctccggtgcg
      901 cgacagcgtg ccggtcaaca gcatcgtccc ggcggtcgaa cccgaggtgg ccggacgttt
      961 cgcgctggcc ggtggctgcc gcaccgcgaa ggtgaaggtg gcagaaccgt cccagacgtt
     1021 ggcggacgac ctggcccggg tcgaggccgt ccgggacgcc ttggggccgc acggccgggt
     1081 gcgcgtcgac gccaacgggg cctggggcgt ggacgaggcg ctggcggcga tccggcagtt
     1141 ggcgcgcttc gatctggagt acgtggagca gccctgcgcc acggtggagg agttggccga
     1201 tctgcgggtg cggctggctc gtgtcggggt gcaggtgcgg atcgcggccg atgagtcgat
     1261 ccgccgcgcg gaggacccgg gccgggtggt ggcgctggcc gccgcggacg tggctgtgct
     1321 caaggtgcag ccgttgggtg gggtacggcg atgcctgcac ctggccgagc agttgggcct
     1381 gccggtggtg gtgtcatcgg cgatcgaaac gtccgtcggc atcgccgcgg gggtcgcgct
     1441 ggccgccgcg ctaccgcagt tgccgttcgc ctgcgggctg aacacgctgg cgctgctctc
     1501 gaccgacgtc gccgacgatc cgctggtggt ccgcgacggg caactggacg tgcggagggt
     1561 cgcccccggt ggtgtgggct ggcaggcttc cgatgaggtg gaccggtggt ggcagcagcg
     1621 acgtcgcact gtcgaggccg gcgctgggca ggtggcgggg cgatgaatga cggcacgatc
     1681 cggccgggtg gcggcgcgac cggctcttcc gcgtgcgcgg ccacgatcgt ggcgcagctg
     1741 gtggtgcagg gcgtccgcga ggtggtgctg gccccgggtt cacgcagtgc gccgttggcc
     1801 ctggccttgg cgcgggcgga ggcccgcgat gaggtgcggc tgcacgtgcg ctacgacgag
     1861 cgcagcgccg gctttctggc gttggggctg gcgaaggcgt ccggggtgcc ggtggcggtg
     1921 gtgaccacgt ccggtaccgc ggtcggcaac ctgctgccgg cggtgatgga ggcccatcac
     1981 gccggggtgc cgttgctcgt ggtgagcgcg gatcgtccgg cggccatggt tgggttcggc
     2041 gccaaccaga ccaccgacca ggtccggctg ttcgagccgt tcgtgcgctg gtcggccagg
     2101 gtgtcgtcgg cggcaccgtc cgaatcatgg ggtgcgcagg cggcgcgcgc ctggctgatc
     2161 gcgacggggg tgctgagccg ccacccgggc ccggtgcacc tcaacgtcga gttggccgag
     2221 ccgctggtgg gcgcggcgcc tgagccgcag gtgcaggcgg tatcggcggc ggctgcgccc
     2281 ggcgccgagc cgctggtgct gccgtccggg ccgcggacgc tactggtgtg cggcgacgcc
     2341 tccgtcgagg taggcacctc ggttgcggag ctggccgttg cggcaccgct gccgctggtg
     2401 gcggagccgt ccagcaatgc gcgtgccgcc gcccttcaca ccggacgcct gctgctcggc
     2461 gggccgctcg cgcagcgggt gcaacgggtg gtggtgttcg ggcatccgac cctgtcccgg
     2521 ccggtcaatc agctgttggc aaggcctgat gtcgaactca tcgtggtcag cccgacctcg
     2581 gactgggtcg atcccggctg gcgggccagc cgggtggcct ccgcggtcgg catcgaacgc
     2641 gacgacagtt cgtgggcgga ggagtgggca gcggcggacg tccgcttgat cgccgcgctg
     2701 aacgagctgg tgggcgcgca gcaaaccttc acgggcccgg cgctcgccgc atcggtggcc
     2761 gcatcggtgt cgccggggga cgtgctggtg ctgggcagtt cgcagccgat ccgcgacgcc
     2821 gacctggcgc cgctgggccg gcaggtggtc tacgccaacc gggggctggc gggcatcgac
     2881 ggcaccgtct cgacggcggt gggcatcgcg ctcgcgaccg ggcgtccggc cactctgctg
     2941 tgcggagacc tgacctttct gcacgacgtc aacgggttgg cggggatggg ggagcgtcgt
     3001 cccgacctgc gcatcgtggt cgcggacgat tccggcggca gcatcttcgc caccctcgaa
     3061 tacggtgccc ccgagttcgc ggacgcgttc gagcgggtct tcgcgacgcc gtccggggtg
     3121 gacgtggcgg ggatcgcgcg tgcccacggc ctaccagtcg tgcaggtctc gactgcgtcg
     3181 gaactggcga cggcgctgaa ggccccgatc cgcggcgtcg agctgatcgt ggcccgtatc
     3241 gaccgagtcg gacgccgcgc actcgaccag gcgatcaacg ccctcggccg ccgctgaccg
     3301 gggtctcgac gctcgaccag cgatcaaaga ccgatgcttg aacctgtcga aaccacgcag
     3361 gtattggtct caacaagctc gaccagcagg gatccgtctc gaccagccgg agaggtctcg
     3421 acctagctcg accagcggtg gagtcgcgag ttggccgaag gacgtgagtg gccaacagcg
     3481 aaagtgaagg gggcggaggg gggctctact cgagtcgtgg atgggtctgg ccgccgaggg
     3541 agcctgcgac tgaggctggt tggctcgagt agagcccccc tccgcccccg accccaacgg
     3601 gcccagatct gaacaagccg cccaacggga ttgggtgtat caggtcaacc cccgcagcca
     3661 ccgccgcacg tgccacgtgg cctcgacgtc gtcttcgttg tagtccagta ctcgctgcct
     3721 cgccgccgcc cgggcggcgg ccgtttcgcc cgccacggcc tcgtcgaacc acgcctgact
     3781 gttcagcccg cccggcgtcg catcgcgcca ggcgaacccc gccgccttgg acgccaccac
     3841 cttcaagccc agcccctggg tgccgaagaa gtgcgtacgg atcgacgtga acaggtcgac
     3901 gaaatgcccg cgcccccacc cccgcgccca ttcgagcacc ggcgaaccgc tacgcacggc
     3961 cagtcgttcg agtcgcacca cctcgtagtc ggagtagtgg tacacccgcg cgtccacgcc
     4021 ctcgacgagc gaccggagcc actgcatcgc ggcctcggcc agcgccgcct cggactcgtc
     4081 ggtgagttcg tcgaatgcgc tgaagtgccg gtagtagtgc gagccgtcgc ggctgtccga
     4141 gaccagaaag ccccacaggt acacccggtc gccgaccgag gtctcgatgt cgaggtcgat
     4201 ctcgacggtc gcgcccggca cgtcgatcgg cccctgcgtg acccggtcga agtcgacccc
     4261 gcgcgccatc agcaccgagc gccgccacgc caggcgcagc cggtcctcgg ccccgacccg
     4321 atgcgtcacc cgggccaggt atgagggcag cagcgcctcg agatcggtgg ccgccagctc
     4381 ggtcacggtc gccacgccca ggtcgcgcag cacctgaatc tcgtggacgt cgagcggcga
     4441 cttcgagatg cgcaggctca gatcgtcgtc gtcgagccgg ggacggcaca cgtcccacca
     4501 ctggcaccac ccgcattcgc ggttggcgat cggctgcagc agcggtggat cgccggccgc
     4561 cagggcggag gccgcctcgg ccaactcgac ccggaacgca tgctcggcgt cgtagcgctc
     4621 cagcgacgac acgagttcga cgtcgtaggg gtcggtgcgc tcacgcgggc tgggcggcac
     4681 caccggcagg gtgaggtcga cccacgacag cacccgcccc agctcgggca gttcgtcggt
     4741 gccgaccacg gcggccaatc gcgtggccga cgcccgcccc accgcctgta gcatgcggtg
     4801 atagtgggcc agttgcagca ggtcgttcag ccgccagttg taccggaacc gtcggcccgg
     4861 caggtcgtgc cggctcagcg gatcggtcag gctcgaccag gtcaacaccg ccggctcgcg
     4921 gcgcacgtcg ctgacccggt gacacttgat ctcggccggg tggtagccgc cgtcgtcggc
     4981 gcgcagcagc aggtcggggc gtccgctgcg gtgccgcacc aggtcgcggg gcagcagggg
     5041 ggcgatcagc acctgcaccc ccgccgccat cgccgcgagc gtgatccgtt cctgctcgga
     5101 cgtcggcagt tcacgcaact cgcggcagtc cagcaccgaa ccagtgaagc cgcccagcag
     5161 ccggctcaac acctcggcct cgaagttgac cccgccgtga aagacctcgc gcaccccctc
     5221 gtcggccgtg ctgggcatca ccaggcccgg gtggaacgcg ttgtgggtct tgaccggaca
     5281 actgcgggcg gcgtaggcgt cgagagcgaa cggctgagcc tgccgagccg gctggactgc
     5341 gctcaccctc gcacctcagg ctcgggcgcg gtgcagggcg acgatgcccc cggtcaggtt
     5401 cttccactgc acgtctgccc agcccgctgc cgtcatcaac tcggccagcc gacgctgggc
     5461 cggccaggcc agaatcgact ccgccaggta gtcgtaggcc accgcattgg acgcgccgag
     5521 gcgtgcgacc agcggcaacg cccgcagcgc cacctgctgg taggcccacc gcagcggcgg
     5581 aaaggtcggg gtgctgaact cgcagatcac gatgcggccg cccggacggg tgacccggcg
     5641 cagttcggcc agtgcggcgg cggtgtcttc gacgttgcgc agcccgaacg agatggtgac
     5701 cgcgtcgaag gcggcgtcgg cgaagggcag cgacaacgcg tcggcgttga cgaaggtcag
     5761 gccgggttgt cgctcggccc ccacctgcag catgcccagg ctgagatcgg ccgccaccac
     5821 cagcgcgccc gcgtcggcga acggacgcga tgacgtgccg gtacccgcgg ccaggtcgag
     5881 aatgcgctgc cccggacggg gcgccaccgc ggccagcacc gcccgccgcc agccccggtc
     5941 gagccccccg gtgagcagat cgttgaccag gtcgtagcgg gccgccaccc cgtcgaacat
     6001 cgccgccacg tcggctctgc gcttggccag cgttgccctc gcccgtccag tttccaccac
     6061 ggcggctagc ttgccacgac ccgcggacag tttccgacca gtccctgtcc gtccggatcg
     6121 ccacgtccct tcacggttgc gcgcccgccg gggccggacg atgcggcgtc cgctttcgtt
     6181 gtggtggaga atcgagccca tggccaaacg cagcacagtg ctcaaggtgg ccgggttgag
     6241 caagtcgttc ggtccggtcg tcatcgtcga caacttcaac ctcgaggtgc gcgagggtga
     6301 ggcgatcgct ctcaccggac gcaacggtgc cggcaagtcg acgatcctgc gctgcatcgt
     6361 gggcgccgac caacccgacg ccggcaccgt cgaggtgagc ggcatccgga tgagcgaaac
     6421 caaccccgag gtgcggcgcg acgtggccac cgtgatcgac gacctcgact tctttcccga
     6481 cctcagtgtg gtcgagcacc tcgacctact ggcccgagcc cacggcatcg acgagcccga
     6541 ggccgccgtc gacgaggtgt tgcacgaggt acagctggtg ccgcagtcgg ggcagttgcc
     6601 gggcacgctg tcgtccggcc aacgccgccg gctggctttg gccacggcct tcatccgccc
     6661 ccgcaagctg ctggtactcg acgagcccga gcagcggctc gacgtcgagg gtgtcgagtg
     6721 gctggccgcg cggctgcagg ccgaacgcgc ccgcggcctg gcgatcgtga tggccagtca
     6781 cgagccgcgg ctggtgcaga gcgtggccac ccgcatcgtc cggatcggtg acggtgtcag
     6841 cgccgacgag gttgtcgggt gagcaagaag tcccgcaagc tgatccgctc gtcgcaggtc
     6901 gcaccggcca ccgcgccgac catggagccg gccgccgaac cgaccgcccc ccagcagaac
     6961 ttctggtttc tgcctgatac ggcccctcac cccgtggacg agcgcgacct gcacgacctg
     7021 atcaagcaat ggcgccacgg tcgcgccacc cgctcggtct gggacgccat tcaagacgcc
     7081 tacgtcgcc
//
LOCUS       DAOSUG010000093         7086 bp    DNA     linear   ENV 10-JUN-2024
DEFINITION  MAG TPA_asm: Micropruina sp. isolate AMR_MDS_2195
            NODE_5186_length_7086_cov_4.618404, whole genome shotgun sequence.
ACCESSION   DAOSUG010000093 DAOSUG010000000
VERSION     DAOSUG010000093.1
DBLINK      BioProject: PRJNA925477
            BioSample: SAMN32864262
            Sequence Read Archive: SRR4236673
KEYWORDS    WGS; ENV; Metagenome Assembled Genome; MAG; Third Party Data; TPA;
            TPA:assembly.
SOURCE      Micropruina sp. (wastewater metagenome)
  ORGANISM  Micropruina sp.
            Bacteria; Bacillati; Actinomycetota; Actinomycetes;
            Propionibacteriales; Nocardioidaceae; Micropruina.
REFERENCE   1  (bases 1 to 7086)
  AUTHORS   Abdulkadir,N., Saraiva,J.P., Zhang,J., Stolte,S., Gillor,O.,
            Harms,H. and Rocha,U.
  TITLE     Genome-centric analyses of 165 metagenomes show that mobile genetic
            elements are crucial for the transmission of antimicrobial
            resistance genes to pathogens in activated sludge and wastewater
  JOURNAL   Microbiol Spectr 12 (3), e0291823 (2024)
   PUBMED   38289113
REFERENCE   2  (bases 1 to 7086)
  AUTHORS   Abdulkadir,N.
  TITLE     Direct Submission
  JOURNAL   Submitted (24-MAR-2023) Environmental Microbiology, Helmholtz
            Center for Environmental Research -UFZ, Permoserstr 15, Leipzig
            04318, Germany
COMMENT     The annotation was added by the NCBI Prokaryotic Genome Annotation
            Pipeline (PGAP). Information about PGAP can be found here:
            https://www.ncbi.nlm.nih.gov/genome/annotation_prok/
            
            ##Genome-Assembly-Data-START##
            Assembly Method        :: SPAdes v. 3.11.1
            Genome Representation  :: Full
            Expected Final Version :: Yes
            Genome Coverage        :: Not Applicable
            Sequencing Technology  :: Illumina MiSeq
            ##Genome-Assembly-Data-END##
            
            ##Genome-Annotation-Data-START##
            Annotation Provider               :: NCBI
            Annotation Date                   :: 05/04/2023 04:57:10
            Annotation Pipeline               :: NCBI Prokaryotic Genome
                                                 Annotation Pipeline (PGAP)
            Annotation Method                 :: Best-placed reference protein
                                                 set; GeneMarkS-2+
            Annotation Software revision      :: 6.5
            Features Annotated                :: Gene; CDS; rRNA; tRNA; ncRNA
            Genes (total)                     :: 2,776
            CDSs (total)                      :: 2,750
            Genes (coding)                    :: 2,714
            CDSs (with protein)               :: 2,714
            Genes (RNA)                       :: 26
            tRNAs                             :: 24
            ncRNAs                            :: 2
            Pseudo Genes (total)              :: 36
            CDSs (without protein)            :: 36
            Pseudo Genes (ambiguous residues) :: 0 of 36
            Pseudo Genes (frameshifted)       :: 2 of 36
            Pseudo Genes (incomplete)         :: 32 of 36
            Pseudo Genes (internal stop)      :: 2 of 36
            ##Genome-Annotation-Data-END##
FEATURES             Location/Qualifiers
     source          1..7086
                     /organism="Micropruina sp."
                     /mol_type="genomic DNA"
                     /submitter_seqid="NODE_5186_length_7086_cov_4.618404"
                     /isolate="AMR_MDS_2195"
                     /isolation_source="Activated sludge of municipal
                     wastewater treatment plants"
                     /db_xref="taxon:2737536"
                     /environmental_sample
                     /geo_loc_name="Canada"
                     /lat_lon="49.25 N 123.1 W"
                     /collection_date="2015-06-16"
                     /metagenome_source="wastewater metagenome"
                     /note="metagenomic"
     gene            complement(<1..1113)
                     /locus_tag="PLF56_04515"
     CDS             complement(<1..1113)
                     /locus_tag="PLF56_04515"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_020389406.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_component: GO:0016020 - membrane [Evidence IEA];
                     GO_function: GO:0008519 - ammonium transmembrane
                     transporter activity [Evidence IEA];
                     GO_process: GO:0072488 - ammonium transmembrane transport
                     [Evidence IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="ammonium transporter"
                     /protein_id="HOQ52857.1"
                     /translation="MNILEINSGDTAWILISAALVLLMTPGLAFFYGGMVRAKSVLNM
                     MMMSFISMGVIGVLWILFGYSMAFGNSVGGAGIIGDPLEYAGLKGMLTYSPDAGYPAL
                     AFVAFQACFAIITVALVSGAIADRASFGAWTMFTVLWGVLVYFPAAHWVFAFSSADGT
                     VVGGWIANVIGAIDFAGGTAIHINAGTAALVLALVIGPRVGFGTKPMRPHNMTLVMLG
                     AALLWFGWFGFNAGSAVAANGLASLAWVNTLAATASAMLGWLVVERFRDGHATSLGAA
                     SGIVAGLVAITPAAGSVDPIGALVIGVLAGIACAYAVGLKYKFGYDDSLDVVGVHLVG
                     GLVGTLLIGFFASPLAMTTIADPDDPTKAHAPGVAGL"
     gene            1355..1984
                     /gene="raiA"
                     /locus_tag="PLF56_04520"
     CDS             1355..1984
                     /gene="raiA"
                     /locus_tag="PLF56_04520"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_016667783.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_component: GO:0005840 - ribosome [Evidence IEA];
                     GO_component: GO:0022626 - cytosolic ribosome [Evidence
                     IEA];
                     GO_function: GO:0045182 - translation regulator activity
                     [Evidence IEA];
                     GO_process: GO:0009386 - translational attenuation
                     [Evidence IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="ribosome-associated translation inhibitor RaiA"
                     /protein_id="HOQ52858.1"
                     /translation="MDVLVTGRHCQISDDFRSRVSERLTGVEKLKDRVIRVEVQVSAN
                     SHARQPDQATTVEITLRSRGPVVRAEASADDKLIAFEHALDRLKSQLRRAADRRKVHR
                     GLRAGQQFGEPLLPPVNQSDDEDRPEIRKIAGLEVHGDGPLVVREKLFKATPLTLAQA
                     LDEMELVGHDFFLYVDAETGAPSAVYRRRAYDYGVIHLELEQPAGEKSA"
     gene            2022..3236
                     /locus_tag="PLF56_04525"
     CDS             2022..3236
                     /locus_tag="PLF56_04525"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_012919284.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_function: GO:0019104 - DNA N-glycosylase activity
                     [Evidence IEA];
                     GO_process: GO:0036297 - interstrand cross-link repair
                     [Evidence IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="crosslink repair DNA glycosylase YcaQ family
                     protein"
                     /protein_id="HOQ52859.1"
                     /translation="MAELTRGQARRIALAAQGFGRPRPSGALTLGHVQRVIDRVAQFQ
                     IDSVNVAVRAQYMPLFSRLGPYDSALLDRATARAPRRLFEYWAHAACLIDVHLQPAFR
                     MAMRRRAANERGALSRILAAKPQLVDRVLDDVRRGGPLSARQIENDEVRSRTDWGWNW
                     SEAKHVLEYLFDSGRVSVAGRNGAFERLYDLTERVLPAEILARPDPSDAASYDTLVQR
                     SVQALGVADLKAITDYFYLRGPAVRASLDRLIGNGELEPVGVAGLPQQFWLAAGARRP
                     RRLLGQGLIAPFDTLVHHRPRVLQFFDVHYRIGIYTPPAQREHGYYVYLFLADDEIVA
                     RVDLKADRALGVLLVQSAWLEQHAAHRPGEVASRLLAELRLMADWLGLASVLVQPRGT
                     LSAALAVATRAP"
     gene            3357..6194
                     /gene="secA"
                     /locus_tag="PLF56_04530"
     CDS             3357..6194
                     /gene="secA"
                     /locus_tag="PLF56_04530"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_011754902.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_function: GO:0005524 - ATP binding [Evidence IEA];
                     GO_process: GO:0006605 - protein targeting [Evidence IEA];
                     GO_process: GO:0006886 - intracellular protein transport
                     [Evidence IEA];
                     GO_process: GO:0017038 - protein import [Evidence IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="preprotein translocase subunit SecA"
                     /protein_id="HOQ52860.1"
                     /translation="MDKLLNMGDGRKLKELRKVAAQVAMIEPDYEAMTDEELRGQTDE
                     FRKRLDEGESIDAILPEAFATVREATKRVLGKRPYDVQVMGGVALHRANIAEMKTGEG
                     KTIVALMPSYLNALSGKGVHIVTVNDYLARYQSEQMGRVHHFLGLTVGVILAQMTPAE
                     RRKQYEADITYGTNNEFGFDYLRDNMALSLDDCVQRGHHYAIVDEVDSILVDEARTPL
                     IISGPAEDNHDWYPEFAKIATKLVRDTHYEVDEKKRTVSILEGGITEVENRLGIENLY
                     ESANTPLISYLNNAIKAKELFKRDKDYVIMDGEVLIVDEHTGRTLIGRRYNEGLHQAL
                     EAKEGVEIKDEYQTLATITLQNYFRMYDKLAGMTGTAKTEESEFQKIYGLGVLPIETN
                     KPMIRIDEKDLIYRTEEAKFAAVVDDVAERHEKGQPILIGTASVAKSELLSRALKKAG
                     VKHEVLNAKNHEHEAAIIALAGRKAAVTVATNMAGRGTDIILGGNPEFLADAVLRNKG
                     LDPVESTDEYEAAWPQVLKEIEEQVAAEHDEVVELGGLYVIGSERHESRRIDNQLRGR
                     SGRQGDPGESRFYLSLQDDLMRLFKSDIINWVMQALKVPDDVPIENKRVSGSIQQAQE
                     QVEAQNFEMRKNVLKYDDVMNRQRHAIYGDRRKVLEGADVETQLRGTVDSVVEQYVRG
                     ATEGFVEDWDLEQLWTQMNTLYPVRLDRSEYEGVELTADELVDDFKADAQAAYDRREE
                     ELGEETMRELERQVLLTVLDRKWREHLYEMDYLREGIGLRAMAQRDPLVEYQREGGAM
                     FNSMMEAFMEEVVGFLFNLEVKVQQPVGLVPDASGKAVPMSAEVLDEMIASGPDADSV
                     GSLLADVQPSEPEADAPAAAAAQAKARPVVKAKGLGARRQGSVSYSAPSESGEAVKTG
                     EVADDPYAGVGRNAPCPCGSGKKFKMCHGRR"
     gene            complement(6274..>7086)
                     /locus_tag="PLF56_04535"
     CDS             complement(6274..>7086)
                     /locus_tag="PLF56_04535"
                     /inference="COORDINATES: ab initio
                     prediction:GeneMarkS-2+"
                     /note="Derived by automated computational analysis using
                     gene prediction method: GeneMarkS-2+."
                     /codon_start=1
                     /transl_table=11
                     /product="hypothetical protein"
                     /protein_id="HOQ52861.1"
                     /translation="LGFAWLELVQPDNNTLTVVRWWAVLWFVAVVGGALLVGPRWVAA
                     ADPFEVFAERIATLSPWQRIGDTIHLVNPLRHCCTARVPRGAAAVTATLLGITAFDSF
                     TNTSVWVGWVQSSTLPRELWGTMGLLGMTALVAGSFALAAYGTARGWTDRLAVSLVPI
                     VVGYNLAHYLSLLVLEGQRTAILASDPLGRGWNVFGTAELGVNTGIFDYPDVVAVLQL
                     ALIVLAHLLGVLVAHDRSLVALQGDRIVRGQVPMLLLMIGYTCSGLLLLFSP"
BASE COUNT         1304 a         2309 c         2457 g         1016 t
ORIGIN      
        1 cagtccggcc acacccgggg cgtgggcctt ggtcgggtcg tccgggtcgg cgatggtggt
       61 catggccagc ggcgaggcga agaagccgat cagcagggta ccgaccaggc cgccgaccag
      121 gtggacgccc accacatcga gcgagtcgtc atagccgaac ttgtacttca ggccgaccgc
      181 gtaggcgcac gcgataccgg ccagcacccc gatcaccagc gcaccgatcg ggtcgaccga
      241 gccggcggcc ggcgtgatcg cgaccagacc cgcgacgatg cccgaggccg cacccagcga
      301 ggtggcgtgt ccgtcccgga accgttccac cacgagccag ccgagcatcg ccgacgcggt
      361 cgcggcaagg gtgttcaccc aggccaggct ggccaggccg ttggcagcga cggccgaacc
      421 ggcgttgaag ccgaaccaac cgaaccacag cagggcggcg ccgagcatca ccagggtcat
      481 gttgtgcgga cgcatcggct tggtgccgaa gccgacccga gggccgatca ccagagccag
      541 caccagggcg gcggtgccgg cgttgatgtg gatcgcggtg ccgccggcga agtcgatcgc
      601 accgatcacg ttggcgatcc agccgcccac aaccgtaccg tccgcgctgc tgaaggcgaa
      661 cacccagtgc gcggcgggaa agtagaccag cacgccccag agcacggtga acatggtcca
      721 ggcgccgaag gatgcccggt cggcgatggc gccggacacg agcgcgacgg tgatgatcgc
      781 gaagcaggcc tggaacgcca cgaacgcgag ggccgggtag ccggcgtccg ggctgtaggt
      841 gagcatgccc ttgaggccgg cgtactcgag cgggtcgccg atgatgccgg cgccgcccac
      901 cgagttgccg aaggccatcg agtagccgaa caggatccag agcacaccga tgacgcccat
      961 cgagatgaag ctcatcatca tcatgttgag cacgctcttg gccctgacca tgcccccgta
     1021 gaagaacgcg agaccggggg tcatcaacag caccagcgcg gcactgatga gaatccaggc
     1081 ggtgtcgccg gagttgattt caaggatgtt catggcagaa cactcgggga tcggtgtttc
     1141 cggatcgttc aggaatcgtt acgcgcggat tgcggcgtgt tacagctgtg ttgcgcactg
     1201 ctgctcgacg cacagccgcc accctggctt tggacaggtc cccccagtag ggtcggttca
     1261 tggcgtcacg ctgattcccg cttcggcggg acagtctggc gcttttgtgt tcgcccgctc
     1321 agccgagcgg gcccgatcga ccaaggaggt tgctgtggac gttctcgtca ccggccggca
     1381 ttgccagatc agcgatgatt tccgttcccg ggtcagcgaa cggctgaccg gcgtggagaa
     1441 gctgaaggac cgggtgattc gggtcgaggt gcaggtatcg gccaactcac acgccaggca
     1501 acccgatcag gccaccaccg tcgagatcac gctgcgcagc cgcggcccgg tggtccgcgc
     1561 cgaggccagt gccgacgaca agctgatcgc cttcgagcac gctctggacc ggctgaagtc
     1621 gcagttgcgc cgcgccgccg atcggcgcaa ggtgcaccgc gggctgcggg ccgggcagca
     1681 gttcggcgag ccgctgcttc cgcccgtgaa ccagagcgac gacgaggacc gtcccgagat
     1741 tcgcaagatc gccggtctcg aggtgcatgg cgacggcccc ctggtggtgc gcgagaagct
     1801 gttcaaggcg accccgctga cgctggcgca ggccctggac gagatggagc tcgtgggcca
     1861 cgacttcttc ctctacgtgg acgccgagac cggcgccccc agcgcggtct accggcggcg
     1921 ggcctacgac tacggggtga tccatctcga actggagcag ccggcggggg aaaagagcgc
     1981 ctgagaaagg aactgtcggt tccggtcgtt aacgtgtcgg catggctgaa ctgacccggg
     2041 gccaggcccg gcgcatcgcg ctcgcggcgc agggcttcgg ccgtccgcgg ccttccggtg
     2101 ccctcaccct ggggcacgtg cagcgggtga tcgaccgggt ggcgcagttt cagatcgact
     2161 cggtgaacgt ggccgtccgg gcgcagtaca tgccgttgtt cagccggctc ggcccctacg
     2221 acagcgccct gctcgatcgc gcgacggctc gtgcgcccag acggttgttc gagtactggg
     2281 cgcacgccgc gtgcctgatc gacgtgcacc tgcagccggc gttccgcatg gcgatgcggc
     2341 ggcgtgcggc caacgaacgc ggcgcgctca gccgcattct ggccgcgaag ccgcagttgg
     2401 tcgaccgggt gctcgacgac gtgcggcgcg gcggtccgct gagcgcccgg cagatcgaga
     2461 acgacgaggt gcgcagccgc accgactggg gctggaactg gtccgaggcc aagcacgtgc
     2521 tcgaatacct gttcgactcc ggacgagtgt cggtggcggg tcgtaacggg gccttcgaac
     2581 ggctgtacga cctgaccgaa cgggtgctgc ccgccgagat actggcccgt cccgacccca
     2641 gcgacgcggc ctcgtacgac acgctggtgc agcgctccgt acaggcactg ggggtggccg
     2701 acctcaaggc cattaccgac tacttctacc tgcgcggccc ggcggtgcgc gcctcgctcg
     2761 atcgcctgat cggcaacggt gaactcgaac cggtgggggt ggccggtctg ccgcagcagt
     2821 tctggctggc ggcgggcgcc cgccgtccgc gccggctgct cggccagggg ctgatcgcac
     2881 ccttcgacac cctggtgcac caccgcccgc gggtgctgca gttcttcgac gttcattacc
     2941 gcatcggcat ctacaccccg cccgctcagc gtgagcacgg ctactacgtg tacctgtttc
     3001 tggccgatga cgagatcgtg gcgcgggtcg acctgaaggc cgatcgggcc ctcggggtgc
     3061 tgctggtcca gtccgcgtgg ctggagcagc acgccgccca ccgtcccggc gaggtggcga
     3121 gcagattgct ggccgaactg cggctgatgg ctgactggct gggcctggcg tccgtcctgg
     3181 tgcaaccgcg cggcactctg agcgcagccc tcgctgtcgc gacgcgcgca ccgtgagcga
     3241 ggtcgtgccg ccaccggcgc aatcctggcc gcagccaaac cgagcgccgt gaccgatccg
     3301 gctagtatgg ccgcgaatgt catcaaccag aaagtgttga aaccaccgtg ggtgttctgg
     3361 acaagctgct caacatgggc gacgggcgca agctcaagga gttgcgcaag gtcgccgcac
     3421 aggtcgccat gatcgagccc gactacgagg cgatgaccga cgaggagttg cgcggccaga
     3481 ccgacgagtt tcgcaagcgt ctcgacgagg gcgagtcgat cgatgcgatc cttcccgagg
     3541 cgttcgccac cgtgcgcgag gccacgaaac gcgtgctcgg caagcgtccc tacgacgtgc
     3601 aggtgatggg cggcgtcgca ctgcaccggg ccaacatcgc cgagatgaag accggtgagg
     3661 gcaagaccat cgtcgcgctg atgccgagtt acctgaacgc gctgagcggc aagggtgtgc
     3721 acatcgtcac ggtcaacgac tacctggccc gctaccagtc cgagcagatg ggccgcgtgc
     3781 atcactttct ggggctcacc gttggggtga tcctggctca gatgacgccg gccgagcggc
     3841 gcaagcagta cgaggccgac atcacctacg gcaccaacaa cgagttcggc ttcgactacc
     3901 tgcgcgacaa catggcgctc agcctcgacg actgcgtcca gcgcggccat cactacgcga
     3961 tcgtcgacga ggtcgactcg atcctggtgg acgaggcacg taccccgctg atcatctcgg
     4021 gccccgccga agacaaccac gactggtacc ccgagttcgc caagatcgcc accaaactgg
     4081 tgcgcgacac ccactacgag gtggacgaga agaagcgcac cgtgtcgatc ctcgaggggg
     4141 gtatcaccga ggtcgagaac cggctgggca tcgagaacct gtacgaaagc gccaacacgc
     4201 cgctgatcag ctacctgaac aacgccatca aggccaagga actgttcaag cgcgacaagg
     4261 actacgtgat catggacggc gaggtgctga tcgtcgacga gcacaccggc cgcaccctga
     4321 tcggacgccg ctacaacgag ggcctgcacc aggcgctcga ggccaaagag ggcgtcgaga
     4381 tcaaggacga gtaccagacc ctcgccacga tcaccctgca gaactacttc cgcatgtacg
     4441 acaagctcgc gggcatgacc ggcacggcca agaccgaaga gagcgagttc cagaagatct
     4501 acggcctggg tgtgctgccg atcgagacca acaagccgat gatccgcatc gacgagaagg
     4561 acctgatcta ccgcaccgaa gaggccaagt tcgccgccgt cgtcgacgac gtcgccgagc
     4621 gccacgagaa gggccaaccg atcctgatcg gtacggcgtc ggtggccaag agcgaactgc
     4681 tcagccgggc gctcaagaag gccggcgtga agcatgaggt gctgaacgcc aagaaccacg
     4741 agcatgaggc ggcgatcatc gctctggcgg gccgcaaggc ggcggtgacg gtggcgacca
     4801 acatggccgg tcgcggcacc gacatcattc tgggtggcaa ccccgagttt ctggccgacg
     4861 cggtcttgcg caacaagggc ctcgacccgg tggagtccac cgacgagtac gaggcggcct
     4921 ggccgcaggt cctcaaagag atcgaagagc aggtggccgc cgaacacgac gaggtggtcg
     4981 aactgggcgg cctgtacgtg atcggttccg agcggcacga gtcgcgccgc atcgacaacc
     5041 agttgcgtgg acgctccggt cgtcagggcg acccgggcga gagccggttc tacctgagcc
     5101 ttcaggacga cctgatgcga ctgttcaagt ccgacatcat caactgggtg atgcaggcgc
     5161 tcaaggtgcc cgacgacgtc ccgatcgaga acaagcgtgt gagcgggtcg atccagcagg
     5221 cgcaagagca ggtcgaggcg cagaacttcg agatgcgcaa gaacgtgctc aagtacgacg
     5281 acgtgatgaa ccgccagcgc cacgccatct acggcgaccg gcgcaaggtg ctcgagggtg
     5341 ccgacgtcga aacgcagctg cggggcaccg tcgattcggt ggtcgagcag tacgtgcgcg
     5401 gggcgaccga gggtttcgtc gaggactggg atctcgagca gctgtggacc cagatgaaca
     5461 ccctgtaccc ggtgcggctc gaccgcagcg agtacgaggg cgtcgagctg accgccgatg
     5521 agttggtgga cgacttcaag gccgatgcgc aggccgccta cgaccggcgc gaagaagagc
     5581 tcggcgaaga gaccatgcgt gaactggagc ggcaggtgct gctgacggtg ctcgaccgca
     5641 agtggcgcga gcatctctac gagatggact acctacgcga gggcatcggc ctgcgggcga
     5701 tggcccagcg cgacccgctg gtcgagtacc agcgcgaggg tggcgccatg ttcaactcca
     5761 tgatggaggc gttcatggaa gaggtggtcg gcttcttgtt caacctcgag gtcaaggtgc
     5821 aacagcccgt cggcctggtg ccggacgcct ccggcaaggc ggtgccgatg tcggccgagg
     5881 tactcgacga gatgatcgcc tccgggcccg atgccgattc ggtgggcagc ctgctcgccg
     5941 acgtgcagcc ctccgagccc gaggcggacg cgcccgcggc cgcggcggcg caggcgaagg
     6001 cgcgtccggt agtgaaggcc aaggggctgg gtgcgcgccg gcagggctcg gtgagctact
     6061 cggcaccgtc cgagtcgggc gaggcggtca agaccggcga ggtcgcggac gatccgtacg
     6121 cgggcgtggg ccgcaacgcg ccttgcccgt gcgggtcggg taagaagttc aagatgtgcc
     6181 acggacgccg ctgacggctt caccactcgt catcccggcg aacgcatcgt cgtcccggcc
     6241 ggcgcaccgg acgtccgatc gggtgtcgac aactcagggt gagaacagca gcagcaggcc
     6301 ggagcaggta tagccgatca tcagtagcag catcggcacc tggccgcgca cgatgcggtc
     6361 gccctgcagc gcgaccagcg agcggtcgtg ggccaccagc accccgagca ggtgtgccag
     6421 cacgatcagg gcgagttgta gcacggcgac gacgtcgggg tagtcgaaga tgccggtgtt
     6481 cacgcccagt tcggcggtgc cgaacacgtt ccagccgcgc cccagcgggt cggaagccag
     6541 aatcgccgtc cgctggcctt cgagtaccag caggctcagg tagtgggcga ggttgtagcc
     6601 gaccacgatg ggcaccaggc tgacggccag ccggtccgtc cagccgcgcg ccgtgccgta
     6661 cgcggccagc gcgaagctac cggcgaccag cgcggtcatg cccagcaggc ccatggtgcc
     6721 ccacagctcg cgcggcaggg tcgaactctg tacccagccg acccagacgc tggtgttggt
     6781 gaagctgtcg aaggcggtga tgcccagcag tgtcgcggtg accgctgcgg caccgcgcgg
     6841 tacccgggct gtacagcagt ggcgcagcgg attgaccaga tggatcgtgt cgccgatccg
     6901 ctgccagggc gacagcgtgg cgatgcgctc ggcgaacacc tcgaaggggt cggcggcggc
     6961 gacccagcgt ggcccgacga gcagcgcacc gcccacaacg gccacgaacc acagcaccgc
     7021 ccaccagcgc accacggtca gggtgttgtt gtccggttgc acgagttcga gccaggcgaa
     7081 gcccag
//
LOCUS       DAOSUG010000094         7076 bp    DNA     linear   ENV 10-JUN-2024
DEFINITION  MAG TPA_asm: Micropruina sp. isolate AMR_MDS_2195
            NODE_5195_length_7076_cov_4.064378, whole genome shotgun sequence.
ACCESSION   DAOSUG010000094 DAOSUG010000000
VERSION     DAOSUG010000094.1
DBLINK      BioProject: PRJNA925477
            BioSample: SAMN32864262
            Sequence Read Archive: SRR4236673
KEYWORDS    WGS; ENV; Metagenome Assembled Genome; MAG; Third Party Data; TPA;
            TPA:assembly.
SOURCE      Micropruina sp. (wastewater metagenome)
  ORGANISM  Micropruina sp.
            Bacteria; Bacillati; Actinomycetota; Actinomycetes;
            Propionibacteriales; Nocardioidaceae; Micropruina.
REFERENCE   1  (bases 1 to 7076)
  AUTHORS   Abdulkadir,N., Saraiva,J.P., Zhang,J., Stolte,S., Gillor,O.,
            Harms,H. and Rocha,U.
  TITLE     Genome-centric analyses of 165 metagenomes show that mobile genetic
            elements are crucial for the transmission of antimicrobial
            resistance genes to pathogens in activated sludge and wastewater
  JOURNAL   Microbiol Spectr 12 (3), e0291823 (2024)
   PUBMED   38289113
REFERENCE   2  (bases 1 to 7076)
  AUTHORS   Abdulkadir,N.
  TITLE     Direct Submission
  JOURNAL   Submitted (24-MAR-2023) Environmental Microbiology, Helmholtz
            Center for Environmental Research -UFZ, Permoserstr 15, Leipzig
            04318, Germany
COMMENT     The annotation was added by the NCBI Prokaryotic Genome Annotation
            Pipeline (PGAP). Information about PGAP can be found here:
            https://www.ncbi.nlm.nih.gov/genome/annotation_prok/
            
            ##Genome-Assembly-Data-START##
            Assembly Method        :: SPAdes v. 3.11.1
            Genome Representation  :: Full
            Expected Final Version :: Yes
            Genome Coverage        :: Not Applicable
            Sequencing Technology  :: Illumina MiSeq
            ##Genome-Assembly-Data-END##
            
            ##Genome-Annotation-Data-START##
            Annotation Provider               :: NCBI
            Annotation Date                   :: 05/04/2023 04:57:10
            Annotation Pipeline               :: NCBI Prokaryotic Genome
                                                 Annotation Pipeline (PGAP)
            Annotation Method                 :: Best-placed reference protein
                                                 set; GeneMarkS-2+
            Annotation Software revision      :: 6.5
            Features Annotated                :: Gene; CDS; rRNA; tRNA; ncRNA
            Genes (total)                     :: 2,776
            CDSs (total)                      :: 2,750
            Genes (coding)                    :: 2,714
            CDSs (with protein)               :: 2,714
            Genes (RNA)                       :: 26
            tRNAs                             :: 24
            ncRNAs                            :: 2
            Pseudo Genes (total)              :: 36
            CDSs (without protein)            :: 36
            Pseudo Genes (ambiguous residues) :: 0 of 36
            Pseudo Genes (frameshifted)       :: 2 of 36
            Pseudo Genes (incomplete)         :: 32 of 36
            Pseudo Genes (internal stop)      :: 2 of 36
            ##Genome-Annotation-Data-END##
FEATURES             Location/Qualifiers
     source          1..7076
                     /organism="Micropruina sp."
                     /mol_type="genomic DNA"
                     /submitter_seqid="NODE_5195_length_7076_cov_4.064378"
                     /isolate="AMR_MDS_2195"
                     /isolation_source="Activated sludge of municipal
                     wastewater treatment plants"
                     /db_xref="taxon:2737536"
                     /environmental_sample
                     /geo_loc_name="Canada"
                     /lat_lon="49.25 N 123.1 W"
                     /collection_date="2015-06-16"
                     /metagenome_source="wastewater metagenome"
                     /note="metagenomic"
     gene            <1..359
                     /locus_tag="PLF56_04540"
     CDS             <1..359
                     /locus_tag="PLF56_04540"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_016669725.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_function: GO:0003723 - RNA binding [Evidence IEA]"
                     /codon_start=3
                     /transl_table=11
                     /product="ANTAR domain-containing protein"
                     /protein_id="HOQ52862.1"
                     /translation="MLTAFSQRELVERAREAGAMAYVVKPFDASDVVPAIEVAMARYA
                     EIRAVEEEVEDLQERFESRKLVEQAKGLLQEGLGLTEPEAFRWIQKTAMDLRKSMREV
                     AEGVIDHAKAGKKPKR"
     gene            complement(550..1308)
                     /locus_tag="PLF56_04545"
     CDS             complement(550..1308)
                     /locus_tag="PLF56_04545"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_012921162.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_function: GO:0008276 - protein methyltransferase
                     activity [Evidence IEA];
                     GO_function: GO:0008757 - S-adenosylmethionine-dependent
                     methyltransferase activity [Evidence IEA];
                     GO_process: GO:0018364 - peptidyl-glutamine methylation
                     [Evidence IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="putative protein N(5)-glutamine
                     methyltransferase"
                     /protein_id="HOQ52863.1"
                     /translation="MSGPDEVVAALRAAGCVYAEDEAALLLAASGPLDELLARRIAGE
                     PLEQVLGWAAFDGLRVRVRPGVFVPRRRSELLVRLAADGLPPDAVVIDLCCGSGALGA
                     ALRLRAPGVELHACDIDPVAVVCARSNLAGASVHRGDLWSALPDSLRGRVAVALANAP
                     YVPTHGVALMPSEARDHEPRAALDGGSDGLDVHRRIAADASAWLAPGGRLIIEVARSQ
                     VAAATAVFAEAGLAAEVHADDDLDATAVVGTAPV"
     gene            complement(1305..1709)
                     /locus_tag="PLF56_04550"
     CDS             complement(1305..1709)
                     /locus_tag="PLF56_04550"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_020388828.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_function: GO:0016790 - thiolester hydrolase activity
                     [Evidence IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="hotdog fold thioesterase"
                     /protein_id="HOQ52864.1"
                     /translation="MSLPDWADGIYSALDAKMGIELFELSATRSTGRMPVEGNTQPFG
                     FWHGGASCVLAETLASLSAYAEVGQGGQIFGVDINATHHRAARDGWVNGVATALHVGG
                     RLGTWEVVLSNDKGQRLCTARVTCALQRPRRG"
     gene            1950..4631
                     /gene="polA"
                     /locus_tag="PLF56_04555"
     CDS             1950..4631
                     /gene="polA"
                     /locus_tag="PLF56_04555"
                     /EC_number="2.7.7.7"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_008362936.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_function: GO:0003676 - nucleic acid binding [Evidence
                     IEA];
                     GO_function: GO:0003677 - DNA binding [Evidence IEA];
                     GO_function: GO:0003887 - DNA-directed DNA polymerase
                     activity [Evidence IEA];
                     GO_function: GO:0008408 - 3'-5' exonuclease activity
                     [Evidence IEA];
                     GO_process: GO:0006261 - DNA-templated DNA replication
                     [Evidence IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="DNA polymerase I"
                     /protein_id="HOQ52865.1"
                     /translation="MSDESGTLLLIDGHSVAYRAFFALPVENFSTTTGQHTNAVYGFT
                     SMLINVLRDEQPTHLAVAFDVSRQTFRKQLYSEYKANRSKSPDEFTGQVALIKEVLNA
                     LNVTHVELDGFEADDIIATLAKQAEAQGLKVLICTGDRDTLQLVTDNVTVLYPRKGVS
                     DLARMTPESVMEKYFVPPAQYPELAALVGETSDNLPGVPGVGPKTAAKWIASYDGLEN
                     VVRRAAEIPGKAGESFRAHLDDVVRNRELNALVGDLELPVRIDQLERLDWNREAVHTV
                     FDGLEFRVLRDRLMETLPAEQTAPTGGFEVVGDVLEPGGLSDWLTTHGQGDLTGLDVT
                     GTWRAGAGDVTGLALAAADGAACWLDVTELTPDDDHALAVWLADESARKALHDAKGPL
                     LAIWSRGWQLNGLASDTQLAAYLLRPDQRIYDLGDLTARHLKRELSAEAPTAQPSDQL
                     AFDFDDPNEGQDSMIRARAVIDLAIALESELDERGERTLLADVELPLLRTLAVMERDG
                     IAVDADYLGQLHSDFDSAVRNAEADAYAVLGRPINLGSPKQLQEVLFDELGMPKTRRT
                     KTGYTTDAESLEGLFVKTEHPFLAHLLRHRDQIRLRQTVEGLQKSIADDGRVHTTYIQ
                     TIAATGRLSSTDPNLQNIPIRTEEGRRIRRAFVVGDGYDTLLTADYSQIEMRIMAHAS
                     EDAGLIEAFHSGEDFHTVMAARVFGVAPEAVDTRMRARIKAMNYGLAYGLSAYGLSQQ
                     LKIEVSEAKGLMDEYFERFGNVRDYLREIVRTAGKTGYTETLLGRRRYLPDLTSSNRQ
                     RREMAERMALNAPIQGSAADIIKVAMLDVEAALAQQGLASRMLLQVHDELVFEVASGE
                     AEVLEALVRDKMGHAVDLSVPLEVSVGTGRSWHEAAH"
     gene            complement(5244..5810)
                     /locus_tag="PLF56_04560"
     CDS             complement(5244..5810)
                     /locus_tag="PLF56_04560"
                     /inference="COORDINATES: protein motif:HMM:NF037041.2"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="hypothetical protein"
                     /protein_id="HOQ52866.1"
                     /translation="MSGTAKIYEAELNPGKIELLAQWLPSQSWFTGDASDLQRVAAFR
                     FVDPDGEVGIETLLVRSGDVLYQVPLTYRAEPLDDADAYLVGTVDHSALGKRWVYDAT
                     GDPVYVAELIRVIHEADTEADLSRGEKTMTVVGSGITSVSNAAMEAARLVRVLDGKHI
                     QTAIPLGMLTGTWTENDEERSEVLATIR"
     gene            6053..>7076
                     /locus_tag="PLF56_04565"
     CDS             6053..>7076
                     /locus_tag="PLF56_04565"
                     /inference="COORDINATES: protein motif:HMM:TIGR00971.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_component: GO:0009897 - external side of plasma
                     membrane [Evidence IEA];
                     GO_component: GO:0030288 - outer membrane-bounded
                     periplasmic space [Evidence IEA];
                     GO_component: GO:0055052 - ATP-binding cassette (ABC)
                     transporter complex, substrate-binding subunit-containing
                     [Evidence IEA];
                     GO_function: GO:0015419 - ABC-type sulfate transporter
                     activity [Evidence IEA];
                     GO_function: GO:0043199 - sulfate binding [Evidence IEA];
                     GO_process: GO:0008272 - sulfate transport [Evidence IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="sulfate ABC transporter substrate-binding
                     protein"
                     /protein_id="HOQ52867.1"
                     /translation="MADDSGTVRAIDRSCFTMTHSRRAVLTALTATVALAMTGCAGGG
                     ASDVAGSTASAPAGGGTVLNLYAYAVPKVGFDKVIPAFNATPAGAGVQFQQSYGASGD
                     QSRKVAAGAEADIVNFSVTPDVTRLVDAGLVDASWDAGEHKGIPFGSVVTIVTRKGNP
                     KGIKDWDDLLKPGVEVVTPNPFSSGSAKWNLLAPYAAKSDGGKNPQAGLDYLDKLITD
                     HVKVQPKSGREATETFLQGTGDVLLSYENEALFIESEGDPVTHVTPPTTFKIENPVAV
                     LKNSKNLQTAEAFRDYLYTPAAQKLWAEAGFRPVDATVAKEFASKFPEPTKLWTIADL
                     GGWPAVD"
BASE COUNT         1190 a         2429 c         2374 g         1083 t
ORIGIN      
        1 tcatgctgac cgccttctcg cagcgcgaac tggtcgagcg ggcccgtgag gccggtgcca
       61 tggcctacgt ggtcaagccc ttcgacgcct cggacgtggt gcctgcgatc gaggtggcga
      121 tggcccgtta cgccgagatc cgtgcggtcg aagaagaggt cgaagacctg caggaacggt
      181 tcgaatcccg caagctggtc gagcaggcca agggcctgct gcaggagggt ctcgggctca
      241 ccgagccgga ggcgttccgg tggatccaga agaccgccat ggacctgcgc aagtcgatgc
      301 gcgaggtcgc cgagggcgtc atcgaccacg ccaaggcggg caagaagccc aagcgctgag
      361 tcgtggacgg ccggtcgtcc gagcggccgg atcgacgtcc tccggcgcca cgggcgggtg
      421 tccgcgtgac gagggcacct acggtgcacg cagcgtgccc gaacgtgtgc gcatcggccg
      481 gtgcgcctcg gcccggcgtg gcagagtggc gcacgcgtca gcccttgata ctcagcgcag
      541 aatcggccct cataccggtg cggtaccgac cactgcggtc gcatcgaggt cgtcgtcggc
      601 gtgcacttcg gcagccaggc cggcctccgc gaacacggcc gtcgccgccg ccacctgcga
      661 gcgcgccact tcgatgatca accggccacc gggtgccagc caggccgacg cgtccgccgc
      721 gatccggcga tgcacgtcca ggccgtccga gcccccgtcc aacgccgctc gcggttcgtg
      781 gtcgcgggcc tcggacggca tcagggccac gccatgggtg ggcacgtagg gtgcgttggc
      841 caacgcgacc gccacccgtc cacgcaacga atcgggcagc gcggaccaca gatcgccccg
      901 atgcaccgac gcccccgcca ggttgctgcg ggcgcacacc acggcgaccg ggtcgatgtc
      961 gcaggcgtgc agttccacac cgggcgcccg gaggcgcagc gccgcgccga gtgccccgct
     1021 gccgcagcac aggtcgatca cgaccgcgtc gggcggcagt ccgtccgcgg ccaatcgcac
     1081 cagcagctct gagcggcgac ggggcacgaa gacccctggc cgaacccgca cccgcaggcc
     1141 gtcgaaggcc gcccatccca gtacctgttc gagaggttcg ccggcgattc ggcgagccag
     1201 cagctcgtcc aggggtccgg acgcggcgag cagcagcgcg gcctcatcct cggcgtacac
     1261 gcagccggcg gcgcgtaggg ccgccaccac ctcgtccggg ccgctcaccc gcgtcgggga
     1321 cgttgcaacg cgcaggtcac ccgggccgtg cacagtcgtt ggcccttgtc attgctgagc
     1381 accacctccc aggtgcccag ccggccgccg acgtgcaacg cggtcgccac cccgttcacc
     1441 cacccgtccc gggccgcccg gtgatgggtg gcgttgatgt cgacgccgaa gatctgaccg
     1501 ccctggccga cctcggcgta ggccgacagc gaggcgagcg tctcggcaag cacgcaggag
     1561 gcgccgccgt gccagaagcc gaacggctga gtgttgccct cgacgggcat gcgtccggtc
     1621 gagcgggtgg cggacagttc gaaaagctcg atgcccatct tcgcgtccag cgcgctgtag
     1681 atgccgtcgg cccaatcggg aaggctcatg ggggccacga ttgcatactt gccgagcgct
     1741 gcgggacacc cgggccgggc cgtagagccg agcaggacca gcgtcggacg acccggcgcc
     1801 tccagccgac gcgacgagtg gtggcacggg gtctgtgccg gtcgtcgccg gctggcgggg
     1861 catcgtcgag cccgtccgca atggtgccgg cgacaacgtg gacgagcagc ggtcagttct
     1921 tgtcggcggc ctccgctacc ctgcgttggg tgagtgacga gtcgggcacg ctgctgctga
     1981 tcgatggcca ttcggtggcc taccgggcgt tcttcgccct gcccgtcgag aacttctcca
     2041 ccactacggg ccagcacacc aacgcggtct acgggttcac gtccatgctg atcaacgtgc
     2101 tgcgtgacga gcagcccacc cacctcgcag tcgcgttcga cgtgtcgcgg cagacgtttc
     2161 gcaagcagct ctacagcgag tacaaggcca accgctccaa gtcgcccgac gagttcaccg
     2221 gccaggtcgc cttgatcaaa gaggtgctga acgcgctcaa cgtcacgcac gtcgaactgg
     2281 acgggttcga ggccgacgac atcatcgcca ccctcgccaa gcaggccgag gcgcaggggc
     2341 tcaaggtgct gatctgcacc ggcgaccgcg acaccctgca gttggtcacc gacaacgtca
     2401 ccgtgctgta cccgcgcaag ggcgtctccg acctggcgcg catgacaccc gagtcggtga
     2461 tggagaagta cttcgtgccg ccggcccagt accccgaact ggcggcgctg gtcggtgaga
     2521 cctccgacaa cctgccgggc gtgcccgggg tcggccccaa gaccgcggcc aagtggatcg
     2581 ccagctacga cggcctcgag aacgtggtgc gccgcgcggc cgagattccg ggcaaggcgg
     2641 gggagtcgtt ccgggcccat ctcgacgacg tggtccgcaa tcgtgagttg aacgcgctgg
     2701 tcggtgatct tgagctgccg gtccgtattg atcagctcga acgccttgac tggaaccgtg
     2761 aagcggtgca caccgtcttc gacgggttgg agttccgggt cttgcgcgat cggttgatgg
     2821 aaaccctgcc cgccgaacag accgctccca ccggtggttt cgaggtggtc ggcgacgtgc
     2881 tcgaacccgg cggcctcagc gattggctga ccacccacgg gcagggcgac ctcaccggtc
     2941 tcgacgtgac cggtacctgg cgggcgggcg cgggcgacgt caccggcctg gccctggctg
     3001 cggccgatgg tgccgcctgc tggctcgacg tcaccgagct gactcccgac gacgaccacg
     3061 cactggccgt ctggttggcc gacgagagcg ccagaaaggc cctgcatgac gccaaggggc
     3121 cgctgctggc gatctggtcg cgcgggtggc aactgaacgg gctggccagc gacactcaac
     3181 tggccgccta cctgcttcgc cccgaccagc gcatctacga cctgggcgac ctgaccgccc
     3241 ggcacctcaa acgcgagttg agcgccgagg cgcccacggc gcagccgtcc gaccagttgg
     3301 ccttcgattt cgacgacccg aacgagggac aggactcgat gatccgggca cgagccgtga
     3361 tcgatctggc catcgccctc gaaagcgaac tggacgagcg cggtgagcgc acgctgctcg
     3421 ccgacgtcga gttgccgctg ctgcgcaccc tggcggtgat ggaacgcgat ggcatcgcgg
     3481 tcgacgcgga ctacctgggg cagttgcaca gcgacttcga ctcggccgtc cgcaacgccg
     3541 aggccgacgc ctacgccgtg ctgggccggc cgatcaacct cggctcgccc aagcagctcc
     3601 aagaagtgtt gttcgacgag ttgggcatgc ccaagacccg gcgcaccaaa accggctaca
     3661 ccaccgacgc cgagtcgttg gagggtctgt tcgtcaagac cgagcacccg tttctggccc
     3721 acctgctgcg gcaccgcgac cagatccggc tgcggcagac cgtcgagggc ctacagaagt
     3781 cgatcgcgga cgacggccgt gtgcacacca cctacattca gaccatcgcc gccaccgggc
     3841 gactcagttc gaccgacccg aacctgcaga acattccgat ccgcaccgag gagggccggc
     3901 gcattcggcg ggcgttcgtg gtgggtgacg ggtacgacac gctgttgacg gccgactaca
     3961 gccagatcga gatgcgcatc atggcgcacg cctccgaaga cgcaggcctg atcgaggcgt
     4021 tccattcggg tgaggacttt cacaccgtga tggcggcccg ggtgttcggc gtggcgcccg
     4081 aggcggtcga tacccgcatg cgcgcccgga tcaaggcgat gaactacggg ttggcgtacg
     4141 ggctgagcgc gtacgggttg tcgcagcagc tcaagatcga ggtttccgag gccaaaggcc
     4201 tgatggatga gtacttcgaa cggttcggca acgtgcgcga ctacctgcgc gagatcgtcc
     4261 gtaccgccgg caagacgggc tacaccgaaa cactgctcgg tcgccgccgg tacctgcccg
     4321 acctcacgtc gtccaaccgg caacgccgcg agatggccga gcgcatggcg ctgaacgccc
     4381 ccatccaggg ctcggccgcc gacatcatca aggtagccat gctcgacgtc gaggctgcgc
     4441 tggcccagca ggggctggcc tcgcgcatgc tgctgcaggt gcacgacgaa ctggtcttcg
     4501 aggtcgcatc cggtgaggcc gaggtgctgg aagccctggt cagagacaag atgggccacg
     4561 ccgtcgacct gtccgtgccg ctggaggtgt ccgtcggcac cggacggtcg tggcacgagg
     4621 ccgcccactg agctgagcgt gggtcgggac cgctgctggt cgcgtgcctc gtcagtcttg
     4681 ggtggttgcg tttggttggg tttgttgccg cggggctgga gtggtcgttc gcgactgtcg
     4741 gttgcggcag taccgacttc gtcgtccctg ccgcaagtgc ttcgtagtgt cgcttcacga
     4801 tcactccagc cccgcttcgg gtgacgccac cgctggtcga ggttgccggg accgcgccgg
     4861 tcgagcttgt cgagactctc gtccgcgggg ccacgtcatt gcgcggtcga gggttggttg
     4921 cacgggttcg tccgcgcgca atgagcgcca agccgcgcaa cgtcatcagg gacggtcggt
     4981 gggaaccccg tggcttctgc gacattgcct tcgatacgtg acgttgcccg cgttgcgccg
     5041 caggcaacgt caccgaaggc aacgtcaccg caggcaacgt cacctaacgc aggcaacgtc
     5101 accgcaggca acgtcaccta acgcaggcaa cgtcaccgcc gtccactgac caccaacaca
     5161 gccccgacca acagcccggt gaacggagtt gagaaccgtc cccaactcga ttcacccaac
     5221 acggccgtcc aacaactgcc gcgtcagcga atggtcgcca gcacctcgct gcgctcctcg
     5281 tcgttctcgg tccaggtacc ggtgagcatg cccagcggga tcgccgtctg aatatgcttg
     5341 ccgtcgagta cccgaaccag gcgggccgcc tccatcgccg cattcgacac actggtgatg
     5401 ccgctgccga ccacggtcat ggtcttctcg ccccgcgaca ggtcggcctc ggtgtcggcc
     5461 tcgtgaatga cgcgaatcaa ctcggccacg tagaccgggt cgccggtggc gtcatagacc
     5521 cagcgcttgc ccagcgccga atggtcgacg gtgccgacca ggtaggcgtc ggcatcgtcc
     5581 agcggctcgg cccggtaggt gagcggaacc tggtagagca cgtcgccgga gcgcaccagc
     5641 agggtttcga tgccgacctc gccgtcggga tcgacgaacc ggaaggcggc gacccgctgc
     5701 agatcggagg cgtcgcccgt gaaccacgac tggctcggca gccactgcgc cagcagctcg
     5761 attttgccgg ggttcaactc cgcctcgtag atctttgctg tgccgctcat cttgaatcct
     5821 cctgggtatg acgccgagtg tgcgtcacca cgctagtacg gacggggcgg cacacggtcc
     5881 ggaaccgagc atccagcctg cggcgacgcc tgctcaggcg cgggccatcc gaatggtgag
     5941 atcattggag atggatttga cccggcggat gcaggcggtc aaactcctac cagtcctcta
     6001 gggaatgttt gttattgccc cgatctgaca agaaggtcct cggccgccac cggtggccga
     6061 cgactccggc accgtccggg ccatcgacag gagttgtttc accatgacgc actcacgcag
     6121 agccgttctc accgcactga cggcaaccgt tgccctggcg atgaccggct gcgccggtgg
     6181 tggtgccagt gatgtcgcgg gctccaccgc gtccgctccg gcgggtggag gcacggtgct
     6241 gaacctctat gcctacgccg tgcccaaggt cggtttcgac aaggtcattc cggcgttcaa
     6301 cgccaccccg gccggcgccg gggtgcagtt ccagcagtcc tacggggcct ccggcgacca
     6361 gtcgcgcaag gtcgccgcgg gggccgaggc cgacatcgtg aacttctcgg tcaccccgga
     6421 cgtgaccagg ctcgtcgacg ccggcctggt ggacgccagt tgggacgccg gtgagcacaa
     6481 gggcattccg ttcggctcgg tggtcacgat cgtgacccgc aagggcaacc cgaagggcat
     6541 caaggactgg gacgacctgc tgaagccggg cgtcgaggtg gtcaccccca acccgttctc
     6601 gtcgggttcg gccaagtgga acctgctggc cccctacgcc gccaagagtg atggcggcaa
     6661 gaacccgcag gccggcctcg actacctcga caagttgatc accgatcacg tgaaggtgca
     6721 gcccaagtcc ggacgcgagg cgaccgagac cttcctgcag ggcaccggcg acgtgttgct
     6781 gagctacgag aacgaggcgc tgttcatcga gagcgagggt gacccggtca ctcacgtcac
     6841 cccgccgacc acgttcaaga tcgagaaccc ggtcgcggtg ttgaagaaca gcaagaacct
     6901 gcagaccgcc gaggcgttcc gcgactacct gtacaccccc gcggcccaga agctgtgggc
     6961 cgaggcgggc ttccgtccgg tggacgcaac cgtggctaag gagttcgcca gcaagttccc
     7021 cgagccgacc aagctgtgga cgatcgccga cctgggtggc tggccggccg tcgaca
//
LOCUS       DAOSUG010000095         7060 bp    DNA     linear   ENV 10-JUN-2024
DEFINITION  MAG TPA_asm: Micropruina sp. isolate AMR_MDS_2195
            NODE_5207_length_7060_cov_4.598001, whole genome shotgun sequence.
ACCESSION   DAOSUG010000095 DAOSUG010000000
VERSION     DAOSUG010000095.1
DBLINK      BioProject: PRJNA925477
            BioSample: SAMN32864262
            Sequence Read Archive: SRR4236673
KEYWORDS    WGS; ENV; Metagenome Assembled Genome; MAG; Third Party Data; TPA;
            TPA:assembly.
SOURCE      Micropruina sp. (wastewater metagenome)
  ORGANISM  Micropruina sp.
            Bacteria; Bacillati; Actinomycetota; Actinomycetes;
            Propionibacteriales; Nocardioidaceae; Micropruina.
REFERENCE   1  (bases 1 to 7060)
  AUTHORS   Abdulkadir,N., Saraiva,J.P., Zhang,J., Stolte,S., Gillor,O.,
            Harms,H. and Rocha,U.
  TITLE     Genome-centric analyses of 165 metagenomes show that mobile genetic
            elements are crucial for the transmission of antimicrobial
            resistance genes to pathogens in activated sludge and wastewater
  JOURNAL   Microbiol Spectr 12 (3), e0291823 (2024)
   PUBMED   38289113
REFERENCE   2  (bases 1 to 7060)
  AUTHORS   Abdulkadir,N.
  TITLE     Direct Submission
  JOURNAL   Submitted (24-MAR-2023) Environmental Microbiology, Helmholtz
            Center for Environmental Research -UFZ, Permoserstr 15, Leipzig
            04318, Germany
COMMENT     The annotation was added by the NCBI Prokaryotic Genome Annotation
            Pipeline (PGAP). Information about PGAP can be found here:
            https://www.ncbi.nlm.nih.gov/genome/annotation_prok/
            
            ##Genome-Assembly-Data-START##
            Assembly Method        :: SPAdes v. 3.11.1
            Genome Representation  :: Full
            Expected Final Version :: Yes
            Genome Coverage        :: Not Applicable
            Sequencing Technology  :: Illumina MiSeq
            ##Genome-Assembly-Data-END##
            
            ##Genome-Annotation-Data-START##
            Annotation Provider               :: NCBI
            Annotation Date                   :: 05/04/2023 04:57:10
            Annotation Pipeline               :: NCBI Prokaryotic Genome
                                                 Annotation Pipeline (PGAP)
            Annotation Method                 :: Best-placed reference protein
                                                 set; GeneMarkS-2+
            Annotation Software revision      :: 6.5
            Features Annotated                :: Gene; CDS; rRNA; tRNA; ncRNA
            Genes (total)                     :: 2,776
            CDSs (total)                      :: 2,750
            Genes (coding)                    :: 2,714
            CDSs (with protein)               :: 2,714
            Genes (RNA)                       :: 26
            tRNAs                             :: 24
            ncRNAs                            :: 2
            Pseudo Genes (total)              :: 36
            CDSs (without protein)            :: 36
            Pseudo Genes (ambiguous residues) :: 0 of 36
            Pseudo Genes (frameshifted)       :: 2 of 36
            Pseudo Genes (incomplete)         :: 32 of 36
            Pseudo Genes (internal stop)      :: 2 of 36
            ##Genome-Annotation-Data-END##
FEATURES             Location/Qualifiers
     source          1..7060
                     /organism="Micropruina sp."
                     /mol_type="genomic DNA"
                     /submitter_seqid="NODE_5207_length_7060_cov_4.598001"
                     /isolate="AMR_MDS_2195"
                     /isolation_source="Activated sludge of municipal
                     wastewater treatment plants"
                     /db_xref="taxon:2737536"
                     /environmental_sample
                     /geo_loc_name="Canada"
                     /lat_lon="49.25 N 123.1 W"
                     /collection_date="2015-06-16"
                     /metagenome_source="wastewater metagenome"
                     /note="metagenomic"
     gene            complement(<1..904)
                     /locus_tag="PLF56_04570"
     CDS             complement(<1..904)
                     /locus_tag="PLF56_04570"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_014847487.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_function: GO:0005524 - ATP binding [Evidence IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="ATP-binding cassette domain-containing protein"
                     /protein_id="HOQ52868.1"
                     /translation="MIEVTDLKKVYRQPNREIRALDGVTLTVPDDSVHGVIGQSGAGK
                     STLVRCIAMLDRPTSGTVAINGADLTSVHASELRRARRRLGLVFQNANLFDSRTIAAN
                     VAYPLELAGVGRAERTAKAQELLGLVGLAATGEAFPAQLSGGQRQRVGIARALATDPD
                     VLLCDEPTSALDPATTGEILDLIGAIKARLGLAVLVITHEMSVVKRICDSVSLLTDGR
                     VVESGALVEVVPRFGSRLSELLLALPPTADVATADGSTVEVLDTITDGATPALTALGR
                     ELGVDLPVVAGTVETLGGVTFARHR"
     gene            complement(1487..2992)
                     /locus_tag="PLF56_04575"
     CDS             complement(1487..2992)
                     /locus_tag="PLF56_04575"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_013864310.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_component: GO:0005886 - plasma membrane [Evidence IEA];
                     GO_process: GO:0006855 - xenobiotic transmembrane
                     transport [Evidence IEA];
                     GO_process: GO:0009410 - response to xenobiotic stimulus
                     [Evidence IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="DHA2 family efflux MFS transporter permease
                     subunit"
                     /protein_id="HOQ52869.1"
                     /translation="MSDNDAARNPWPALWALIIGFFMLLVDTTIVSVANPAIKAALDP
                     TTNNLDNVVWVTSAYLLAYAVPLLIAGRLGDAFGPKRVYLTGLAVFTLASLGCGLSTS
                     LSMLVAMRAAQGLGAALMTPQTMALITRTFPPNHRGAAMGLWGATAGVATLVGPIAGG
                     LLVDAFGWEAIFFVNLPVGVVGFVLAWLLVPRLETHPHRFDLLGVVLSAAGLFLLVFG
                     LQEGENYAWGTIWGPVSVWSMIIAGVALLAVFVWQQSRTTSEPLIPLELFRDRNFSVS
                     NLAIVTVGFAVTAMSLPMMFFLQLARGLTPTDAALLTIPTAVLSGVLSPIAGNILDRT
                     DPRYLLVPGLLMVAGGQFWYASLMRADTPLWQFLLPAAVIGIGSAGMWGPLATTATRN
                     LPPRQAGAGSGIYNTTRTMGSVIGSAAIAALMQSRLEANLPGASSSAGFGSGQLPAAV
                     VDGFSAAMAQSLLMPAAVLALGTLTVLLLRRGHVVAHVPQRAIVEVEGSPA"
     gene            complement(2989..3618)
                     /locus_tag="PLF56_04580"
     CDS             complement(2989..3618)
                     /locus_tag="PLF56_04580"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_013864309.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_function: GO:0003677 - DNA binding [Evidence IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="PadR family transcriptional regulator"
                     /protein_id="HOQ52870.1"
                     /translation="MTADCADRLTPLGVMVLALLREGDMHPYEMIRLMRQRHDDRLAP
                     ITNGTLYHTVARLQKLGLLAEQGVDRHGNRPERTTYSLTEAGRAIVPEWVRRELPRVD
                     RPDQFRIALAESHNLERSEVLELLEQRRRALSERYTYHRDGLRKASDLAVAPQYLVEI
                     EREEALLEADLRWLDTLIQRLEARNFAWGQDPTGRPSSRYTNEREAAHA"
     gene            complement(3985..4995)
                     /gene="glsA"
                     /locus_tag="PLF56_04585"
     CDS             complement(3985..4995)
                     /gene="glsA"
                     /locus_tag="PLF56_04585"
                     /EC_number="3.5.1.2"
                     /inference="COORDINATES: protein motif:HMM:NF009020.0"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_function: GO:0004359 - glutaminase activity [Evidence
                     IEA];
                     GO_process: GO:0006541 - glutamine metabolic process
                     [Evidence IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="glutaminase A"
                     /protein_id="HOQ52871.1"
                     /translation="MYLPSHPLPDHEQAHRFVSTGTLPDLATVDTLVHEAYRRFANVE
                     EGALSRVYPALATADPAAFGICLAGVSGAVRSVGDVEARFTLMSVTKPFVFALACEAL
                     GIDAVRRLVGVNATGLPFNSAQAVERTSDGRTNPMVNAGAIATTSAIPGATLDERWAA
                     LHEGLSRFAGRPLGMDEATLASARATNFQNRALAMLLGGAGALIGDPLDAVDLYTRQC
                     CLEVSAQDLAIMGATLADGGVHPISGERVVSQVVARASLAVMSIAGMYESSGDWLLDV
                     GMPGKSGISGGVVAISPGKGALGVFSPPLDNEGNSVRGQLVARFLARELGLDVLGSAS
                     AH"
     gene            complement(4998..6494)
                     /locus_tag="PLF56_04590"
     CDS             complement(4998..6494)
                     /locus_tag="PLF56_04590"
                     /inference="COORDINATES: protein motif:HMM:NF012981.2"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_function: GO:0016301 - kinase activity [Evidence IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="diacylglycerol kinase family protein"
                     /protein_id="HOQ52872.1"
                     /translation="MTRADASSTRSWTSVRKQVGALDAALFEAVADTHSPMLDAVMPR
                     LTKVADRSVLWIGIAALCGLSGSRHARRGAVRGLVTLGITSLIANQVSKRLHRRPRPS
                     IAGVPLARLSRRIPSSTSFPSGHSASAAAFATAMGQEAPLLGVPLKALAGAVGFSRVA
                     TGAHYPSDVAAGLLLGTTVAAVIGRLVPPATAPDRLARPRAVQQVTPRPTGQGVTLVI
                     NPHSHSGKAGAMIRPLRRALPELSVIELQPNDDVAAVLRHTAAHTEVLAVAGGDGTVA
                     TAAQAAIDAGTPLAVFPAGTFNHFAKDLRVYPLKAAIAAVREGTVTKVDVGYVNDTLF
                     LNTASVGAYTDFVSIRERYEHRIGKPLAALLAGVRTLRRRKSLLVRIDGVEKRVSLFF
                     LGNGRYRPRGFAPLLRDDLDDGLIDLRMLDTSSRLARLSVLFAVLTGQLEHDRRYSER
                     AVSEVTIELPDGPVRIARDGELGESSDRLHVRVASRALTAYQPRNARD"
     gene            complement(6500..>7060)
                     /locus_tag="PLF56_04595"
     CDS             complement(6500..>7060)
                     /locus_tag="PLF56_04595"
                     /inference="COORDINATES: protein motif:HMM:NF015746.2"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_function: GO:0015558 - secondary active
                     p-aminobenzoyl-glutamate transmembrane transporter
                     activity [Evidence IEA];
                     GO_process: GO:1902604 - p-aminobenzoyl-glutamate
                     transmembrane transport [Evidence IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="AbgT family transporter"
                     /protein_id="HOQ52873.1"
                     /translation="AGTLQGGNEAVTAIAKTFGSLGGLLLMFLMIAQFIALFNWTNLP
                     TVAAVSAAELLQQASVPAWVLLIAFIIVIVILDVILPGLVPKWVIFAPVFIPIFASLG
                     VAPQTLLAAYRVGDSPMNVLTPLMVYLPFMVTVAQRYKKDAGIGTIIALMVPYTMWVL
                     ISWTALYLLWFFLGIPWGPGAPVHLP"
BASE COUNT         1147 a         2342 c         2561 g         1010 t
ORIGIN      
        1 cccgatgccg cgcgaaggtg acgccgccga gggtttcgac ggtgccggcg accaccggca
       61 ggtcgacacc caactcgcgg cccagggcag tgagggcggg tgtcgcaccg tcggtgatgg
      121 tgtcgagaac ctcgacggtg gagccgtcgg cggtcgcgac gtccgcggtg gggggcaggg
      181 ccagcagcag ttcgctgagc cggctgccga agcgcgggac cacctcgacc aacgcaccgg
      241 attcgaccac gcgaccgtcg gtcagtagcg acaccgagtc gcagatgcgc ttgaccacgc
      301 tcatctcgtg cgtgatcacc agcaccgcca ggcccagccg ggccttgatc gcaccgatca
      361 ggtcgaggat ctcaccggtg gtggccgggt cgagcgcgct ggtcggctcg tcgcagagca
      421 gcacgtccgg atccgtggcc agggcgcggg cgatgccgac gcgttggcgc tggccgccgg
      481 agagttgcgc cggaaacgcc tcaccggtcg cggccagacc gaccagcccg agcaactcct
      541 gggccttggc ggtgcgttcg gcgcgtccga ccccggcgag ttcgaggggg taggcgacat
      601 tggcggcgat ggtgcgcgag tcgaagaggt tggcattctg gaacaccagt ccgaggcggc
      661 gccgggccct ccgcaactcg ctggcgtgca ccgaggtgag atctgcaccg ttgatcgcga
      721 cggtgccgga cgtggggcga tcgagcatcg cgatgcagcg caccagggtg gatttgccgg
      781 ctccggactg cccgatcaca ccgtgcacgg agtcgtccgg aacggtcagg gtgacgccgt
      841 cgagcgcgcg gatctcgcgg ttgggttgtc ggtagacctt cttcaggtcg gtgacttcga
      901 tcacggttct gccatctgtc aggggtttgg gcttgggtct ggcgacagac agcggcgccg
      961 gacgaagcgt ccgggtcaca gagagcggaa tcgggcctga tcaggccgaa gttgcgctgt
     1021 cgcagccagg gttcagcgac aacacattcg cgcgcacatc atcgtcggcg ctgccaggag
     1081 cggccggacg gatgggcttg cggtgatgaa cacgcgctca gtctgccaag caccggtccg
     1141 gcaaggcaaa tcgtagtacc aatgacggcc tgggcgaact gaacggagtt aggaaccgtc
     1201 cccccaactc ccgttcggtc cgcacaggtg ggaaccgtcg ctccctttgg tcactcgccc
     1261 acccgaacga accactggca cgaggggtgc cgcctgaaaa gcctgcacca gtggcagacc
     1321 tcgcggcgcg cgcaggcacg accccctgca ctggtgcagg gatttcaggc ggttcccttg
     1381 gcgaccgtgc caaaagctct gctgacgaac gcgacgcaga ccgtcggcga gcgcgtcgag
     1441 cagggctgga cggttggcag gggctgggct accgcgcgga tcgcgatcag gcgggcgagc
     1501 cctccacctc gacgatcgcg cgctggggca cgtgggcgac cacgtgcccg cggcgcagca
     1561 acagcaccgt cagcgtgccc agcgcgagca cggcggcggg catcagcagc gactgcgcca
     1621 tcgccgcact gaacccgtcc accaccgcgg cgggcagctg acccgagccg aagccggcgc
     1681 tggacgatgc ccccggcagg tttgcctcca gccgggactg catcagcgcg gcgatcgccg
     1741 ccgagccgat caccgagccc atggtgcggg tggtgttgta gatgcccgag ccggcacccg
     1801 cctgccgggg cggcaggttg cgggtggccg tggtggccag cggtccccac atgccggcgc
     1861 tgccgatgcc gatcacggcc gccggcagca gaaactgcca caggggggtg tcggcccgca
     1921 tcagcgaggc gtaccagaac tgtccgccgg ccaccatcag caggccgggc accagcaggt
     1981 agcggggatc ggtgcggtcg aggatgttgc cggcgatcgg cgagagaacg cctgagagca
     2041 ccgcggtcgg aatggtgagc agcgcagcgt cggtcggggt gagcccgcga gccaactgca
     2101 gaaagaacat catcggcagt gacatggccg tgaccgcgaa gcccaccgtg acgatggcga
     2161 ggttcgacac cgagaagttg cggtcgcgga acagttccag cggaatcagc ggctcggacg
     2221 tggtgcgcga ctgctgccac acgaacaccg cgagcagcgc cacgccggcg atgatcatcg
     2281 accacactga caccgggccc cagatcgtgc cccatgcgta gttctcaccc tcttgcaggc
     2341 cgaagaccag caggaacagt cccgccgcgc tcagtacgac gccgagcagg tcgaagcgat
     2401 gcggatgggt ctcgagccgc ggcaccagaa gccaggccag cacgaagccg accacgccga
     2461 cgggcaggtt gacgaagaag atggcctccc agccgaaggc gtccaccagg aggccaccgg
     2521 cgatcgggcc gaccagcgtg gcgaccccgg ccgttgcgcc ccacaggccc atcgccgcgc
     2581 cccggtggtt gggcggaaat gtgcgggtga tcagcgccat ggtctggggc gtcatcaaag
     2641 cggcgcccag accctgcgcg gcacgcatcg cgacgagcat gctcagcgag gtcgagagcc
     2701 cgcagccgag cgaggccagg gtgaacacgg ctaggccggt caggtacacg cgcttggggc
     2761 cgaacgcgtc gccgagtcgt ccggcgatca acagcggaac ggcgtaggcg agcaggtagg
     2821 cgctggtcac ccacaccacg ttgtcgaggt tgttggtggt ggggtcgagg gcggctttga
     2881 tcgccgggtt ggccacggac acgatcgtgg tgtcgaccag gagcatgaag aacccgatga
     2941 tcaacgccca gagcgcgggc cagggattgc gtgctgcgtc gttgtcgctc atgcgtgagc
     3001 tgcctctcgt tcgttggtgt agcggctgga cgggcggccc gtggggtcct gcccccacgc
     3061 gaagttgcgc gcctccagcc gttggatcag ggtgtcgagc cagcgcaggt cggcctccag
     3121 cagggcctcc tcgcgttcga tctcgaccag gtactgcggg gcaaccgcga gatcgctggc
     3181 cttgcgtagc ccgtcgcgat ggtaggtgta ccgctcgctc agcgcgcgtc gccgctgctc
     3241 gagcagttcg agcacttcgg agcgctcgag gttgtgcgac tcggccagcg cgatgcggaa
     3301 ctggtcggga cgatcgaccc gcggcagttc gcggcgcacc cactcgggca cgatggcgcg
     3361 tccggcctcg gtgagggagt aggtggtgcg ttcgggacgg ttgccgtgcc ggtcgacgcc
     3421 ctgctcggcc agcaggccga gcttctgcag ccgggcgacc gtgtggtaca gggtgccgtt
     3481 ggtgatcggg gccagccggt cgtcgtggcg ttggcgcatc agccggatca tctcgtaggg
     3541 gtgcatgtcg ccctcacgga gcagggccag caccatgaca cccagaggtg tgaggcggtc
     3601 ggcgcagtcg gcggtcattg gttcgtccgt aatgttcggt gctgattagt ccggatggac
     3661 tatacgtcga agttgatgat tgaacaacac ccgtggatga cggcgacgcc gctggtcgtg
     3721 accccccgtc attccggcgc atgccggggt ctctgcgtgc ggcgagatcc cggggcaagc
     3781 ccgggatgac tattgcggcc ggacagcccg gggttatggc tgcgccgctg ggcttgaccc
     3841 ctcgtcattt cggcgcaggc cggggtctct gcgtgcggcg agatcccggg gcaagcccgg
     3901 gatgacgaag ccgccgctgg ttgtgacgaa gccgccgctg gtcgtgacga agccgccgct
     3961 ggttgtgacg aagccgccgc cggctcagtg cgccgaggcc gagcccagta cgtccaggcc
     4021 caactcgcga gccagaaagc gggcgaccag ttggccgcgg acgctgttgc cctcgttgtc
     4081 gagaggtggc gagaacacgc cgagggcgcc cttgccgggc gagatggcca ccaccccgcc
     4141 gctgatgccg ctcttgccgg gcatgcccac gtcgagcagc cagtcgcccg agctctcgta
     4201 catgccggcg atgctcatca ccgccaggga cgcccgggcc accacctggc tgaccacccg
     4261 ttcaccgctg atcgggtgca cgcccccgtc ggcgagcgtc gcgcccatga tggcgagatc
     4321 ctgcgcggac acctcgaggc agcactgccg ggtgtacagg tcgacggcat cgagggggtc
     4381 gccgatcagg gcgccggcgc cgccgagcag catcgccagc gcgcggtttt gaaagttggt
     4441 ggcgcgtgcc gaggccagcg tggcctcgtc catacccaac ggacgccccg cgaagcgcga
     4501 gagcccctcg tgcaacgccg cccagcgctc gtcgagggtc gcgccgggga tcgcgctggt
     4561 ggtcgcgatc gcgccggcgt tcaccatcgg attcgtgcgg ccgtcggacg tacgctccac
     4621 cgcctgcgcg gagttgaacg gcagcccggt ggcgttcacc ccgaccagtc ggcgcaccgc
     4681 atcgatgccc agcgcctcgc acgcgagggc gaagacgaac ggcttggtga cgctcatcag
     4741 ggtgaatctg gcctcgacgt cgccgacgga gcgcaccgcg ccgctgacgc cggccaggca
     4801 gatgccgaat gcggccgggt cggccgtcgc cagggcgggg tagacccgtg acagcgcgcc
     4861 ttcctcgaca ttggcgaagc gccggtatgc ctcgtgcacc agggtgtcca cggtggcgag
     4921 gtcgggcagc gttccggttg agacgaatcg gtgcgcctgc tcatggtccg gcagtgggtg
     4981 cgaaggcagg tacatggtca gtcccgggcg ttgcggggtt ggtaggcggt cagcgcgcgg
     5041 ctcgccacgc gcacgtgcag ccggtcggac gactcgccca gttcaccgtc gcgggcgatc
     5101 cgcaccggac cgtccggcaa ctcgatggtg acctcggaca cggcgcgctc gctgtagcgc
     5161 ctgtcgtgct ccaactgccc ggtgagtacg gcgaacagca cgctgagccg ggccagccgg
     5221 ctggaggtgt cgagcatgcg caggtcgatc aggccgtcgt ccaggtcgtc gcgcagtagc
     5281 ggggcgaagc cgcgcggccg gtagcgtccg ttgccgagaa agaacaggct gacgcgcttc
     5341 tcgaccccgt cgatgcgcac gagaagcgat ttgcgccggc gcagggtgcg caccccggcg
     5401 agcagcgcgg ccagcggctt gccgatgcgg tgttcgtagc gttcccggat cgacacgaag
     5461 tcggtgtagg cgcccacgct ggccgtgttc agaaagagcg tgtcgttgac gtagccgaca
     5521 tcgaccttgg tcacggtgcc ctcgcgcacc gcggcgatcg ccgccttgag cggatacacc
     5581 cgaaggtcct tggcgaagtg attgaaggta ccggcgggaa acaccgcgag cggcgtaccg
     5641 gcgtcgatgg ccgcctgggc ggccgtcgcg accgtaccgt cgccgccggc caccgcgagc
     5701 acctcggtgt gcgcggccgt gtgtcgcagc accgcggcca cgtcgtcgtt cggttgcaac
     5761 tcgatcacgc tcaactcggg cagggctcgc cgcaacggac ggatcatcgc cccggccttg
     5821 ccgctgtgcg agtgtgggtt gatcaccagc gtcacgccct ggcccgttgg gcgtggcgtc
     5881 acctgctgga cggcccgtgg ccgggccagc cggtcgggcg cggtggccgg gggtacgagg
     5941 cgtccgatca cggcggccac cgtggtgccc agcagcaggc cggcggccac gtccgagggg
     6001 tagtgggcgc cggtcgcgac ccgcgagaag ccgaccgccc cggccagtgc cttcagcggc
     6061 acccccagca gcggcgcctc ctggcccatc gccgtggcga aggcggccgc actggccgaa
     6121 tgcccgctcg gaaacgaggt ggacgacgga atccgccgcg acaaccgagc cagcggcacg
     6181 cccgcgatgc tgggccgggg ccgccggtgc agccgcttcg agacctggtt ggcgatcagg
     6241 ctggtgatgc ccagcgtcac cagccctcgg acggcgccgc gccgggcatg ccggctgccg
     6301 gagaggccgc acagggcggc gatgccgatc cacagcaccg agcggtcggc cactttggtg
     6361 agccggggca tcaccgcgtc caacatcgga ctgtgggtgt cggcgacggc ctcgaaaagc
     6421 gcggcgtcca gggcacccac ctgcttgcgt accgaggtcc aactcctcgt ggacgatgcg
     6481 tctgcgcgag tcacccggct catggtaggt ggaccggcgc gcccggtccc cagggaatac
     6541 ccaggaagaa ccagagcagg tacagcgccg tccacgagat cagcacccac atggtgtagg
     6601 gcaccatcaa cgcgatgatc gtgccgatgc ccgcgtcctt cttgtaccgc tgggccacgg
     6661 tgaccatgaa cggcaggtac accatcagcg gggtgagcac gttcatcggc gaatcaccga
     6721 cccggtaggc ggccagcagg gtctggggcg cgacgccgag ggaggcgaag atcggaatga
     6781 agacgggtgc gaagatcacc catttgggca ccaggccggg caggatcacg tcgagaatca
     6841 cgatcacgat gatgaacgcg atcagcagca cccaggccgg cacgctggcc tgttgcagca
     6901 actccgctgc cgagacggcg gcgacggtgg gcaggttcgt ccagttgaac agggcgatga
     6961 actgggcgat catcaggaac atcagcagca gcccgccgag gctgccgaac gtcttggcga
     7021 tcgcggtcac cgcctcgttg ccgccctgga gcgtgccggc
//
LOCUS       DAOSUG010000096         7054 bp    DNA     linear   ENV 10-JUN-2024
DEFINITION  MAG TPA_asm: Micropruina sp. isolate AMR_MDS_2195
            NODE_5211_length_7054_cov_3.774396, whole genome shotgun sequence.
ACCESSION   DAOSUG010000096 DAOSUG010000000
VERSION     DAOSUG010000096.1
DBLINK      BioProject: PRJNA925477
            BioSample: SAMN32864262
            Sequence Read Archive: SRR4236673
KEYWORDS    WGS; ENV; Metagenome Assembled Genome; MAG; Third Party Data; TPA;
            TPA:assembly.
SOURCE      Micropruina sp. (wastewater metagenome)
  ORGANISM  Micropruina sp.
            Bacteria; Bacillati; Actinomycetota; Actinomycetes;
            Propionibacteriales; Nocardioidaceae; Micropruina.
REFERENCE   1  (bases 1 to 7054)
  AUTHORS   Abdulkadir,N., Saraiva,J.P., Zhang,J., Stolte,S., Gillor,O.,
            Harms,H. and Rocha,U.
  TITLE     Genome-centric analyses of 165 metagenomes show that mobile genetic
            elements are crucial for the transmission of antimicrobial
            resistance genes to pathogens in activated sludge and wastewater
  JOURNAL   Microbiol Spectr 12 (3), e0291823 (2024)
   PUBMED   38289113
REFERENCE   2  (bases 1 to 7054)
  AUTHORS   Abdulkadir,N.
  TITLE     Direct Submission
  JOURNAL   Submitted (24-MAR-2023) Environmental Microbiology, Helmholtz
            Center for Environmental Research -UFZ, Permoserstr 15, Leipzig
            04318, Germany
COMMENT     The annotation was added by the NCBI Prokaryotic Genome Annotation
            Pipeline (PGAP). Information about PGAP can be found here:
            https://www.ncbi.nlm.nih.gov/genome/annotation_prok/
            
            ##Genome-Assembly-Data-START##
            Assembly Method        :: SPAdes v. 3.11.1
            Genome Representation  :: Full
            Expected Final Version :: Yes
            Genome Coverage        :: Not Applicable
            Sequencing Technology  :: Illumina MiSeq
            ##Genome-Assembly-Data-END##
            
            ##Genome-Annotation-Data-START##
            Annotation Provider               :: NCBI
            Annotation Date                   :: 05/04/2023 04:57:10
            Annotation Pipeline               :: NCBI Prokaryotic Genome
                                                 Annotation Pipeline (PGAP)
            Annotation Method                 :: Best-placed reference protein
                                                 set; GeneMarkS-2+
            Annotation Software revision      :: 6.5
            Features Annotated                :: Gene; CDS; rRNA; tRNA; ncRNA
            Genes (total)                     :: 2,776
            CDSs (total)                      :: 2,750
            Genes (coding)                    :: 2,714
            CDSs (with protein)               :: 2,714
            Genes (RNA)                       :: 26
            tRNAs                             :: 24
            ncRNAs                            :: 2
            Pseudo Genes (total)              :: 36
            CDSs (without protein)            :: 36
            Pseudo Genes (ambiguous residues) :: 0 of 36
            Pseudo Genes (frameshifted)       :: 2 of 36
            Pseudo Genes (incomplete)         :: 32 of 36
            Pseudo Genes (internal stop)      :: 2 of 36
            ##Genome-Annotation-Data-END##
FEATURES             Location/Qualifiers
     source          1..7054
                     /organism="Micropruina sp."
                     /mol_type="genomic DNA"
                     /submitter_seqid="NODE_5211_length_7054_cov_3.774396"
                     /isolate="AMR_MDS_2195"
                     /isolation_source="Activated sludge of municipal
                     wastewater treatment plants"
                     /db_xref="taxon:2737536"
                     /environmental_sample
                     /geo_loc_name="Canada"
                     /lat_lon="49.25 N 123.1 W"
                     /collection_date="2015-06-16"
                     /metagenome_source="wastewater metagenome"
                     /note="metagenomic"
     gene            <1..387
                     /locus_tag="PLF56_04600"
     CDS             <1..387
                     /locus_tag="PLF56_04600"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_011530435.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="IS701 family transposase"
                     /protein_id="HOQ52874.1"
                     /translation="RYEWARTRVHGINYPDAVYWLMARRSLADPTDLAYYLCHAPARA
                     SLADLVKVAGIRWTIEETFQTAKGQTGLDHYQVRQYTGWYRHITLSMLAHAYLTVTRS
                     KRGAHQTAPTAWSTSAPPRSGDSSTT"
     gene            complement(515..808)
                     /locus_tag="PLF56_04605"
     CDS             complement(515..808)
                     /locus_tag="PLF56_04605"
                     /inference="COORDINATES: ab initio
                     prediction:GeneMarkS-2+"
                     /note="Derived by automated computational analysis using
                     gene prediction method: GeneMarkS-2+."
                     /codon_start=1
                     /transl_table=11
                     /product="hypothetical protein"
                     /protein_id="HOQ52875.1"
                     /translation="MPPPDDHPRATSGSAISRALAGIAGLACLACCLIPVLLTAGVIG
                     GATWITLGRWMPTIALVLAAAAAGLFWVTARRRRRPVGCGAGCSCDRPAATAA"
     gene            complement(808..1245)
                     /locus_tag="PLF56_04610"
     CDS             complement(808..1245)
                     /locus_tag="PLF56_04610"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_011751658.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_function: GO:0003677 - DNA binding [Evidence IEA];
                     GO_process: GO:0006355 - regulation of DNA-templated
                     transcription [Evidence IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="MerR family transcriptional regulator"
                     /protein_id="HOQ52876.1"
                     /translation="MSAGLRAGQVAAAAGVNLQTLRYYERRGLLDEPDRTLGGHRIYP
                     DQAITLLRVIKTAQRLGFSLSEVAELLETGSHHHGRRDAGLQARAAAKVAEIDRKIAD
                     LTVIRESLVQALDAGCDDLVTCAGSSCCPLPFTELTTTRPEPV"
     gene            complement(1242..1577)
                     /locus_tag="PLF56_04615"
     CDS             complement(1242..1577)
                     /locus_tag="PLF56_04615"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_011751657.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="hypothetical protein"
                     /protein_id="HOQ52877.1"
                     /translation="MTQTEPTWVPEACTLPTAQQPLRLAEFDALFTADVTDVQQPSAD
                     TAILTLRPDPTVAGRAADLAVRETACCSFFEFTLTATGGHLTLQIRTPTAQIEVLDAL
                     VGRARGVAR"
     gene            2240..2443
                     /locus_tag="PLF56_04620"
     CDS             2240..2443
                     /locus_tag="PLF56_04620"
                     /inference="COORDINATES: ab initio
                     prediction:GeneMarkS-2+"
                     /note="Derived by automated computational analysis using
                     gene prediction method: GeneMarkS-2+."
                     /codon_start=1
                     /transl_table=11
                     /product="hypothetical protein"
                     /protein_id="HOQ52878.1"
                     /translation="MTASPPGRSSVAAAGMIRRSLPLAFLTGAGIGILGGMIGLGGAE
                     FRLPLLIGVFGFLALQAVILNRR"
     gene            2440..3069
                     /locus_tag="PLF56_04625"
     CDS             2440..3069
                     /locus_tag="PLF56_04625"
                     /inference="COORDINATES: protein motif:HMM:NF014035.2"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_process: GO:0006810 - transport [Evidence IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="sulfite exporter TauE/SafE family protein"
                     /protein_id="HOQ52879.1"
                     /translation="MSLIVVLTAVPARLVSVPFGDLAPYWYVVVNLLAGSLVGAWIGA
                     TWATRMRSATLYRVIAVLLVFIAAALATTHLAQVGTLDLPPVPRTILGVAAGVGIGVV
                     AAVMGVAGGELLIPTIVLLYGVDIKIAGSLSLVVSLPTMLVAFARYSRDNSFQALRDN
                     KAFVVAMTIGSIAGTIVGGLLLGAVPTLVLIPLLVVLLLLSAVKVWRHT"
     gene            complement(3034..3498)
                     /locus_tag="PLF56_04630"
     CDS             complement(3034..3498)
                     /locus_tag="PLF56_04630"
                     /inference="COORDINATES: protein motif:HMM:NF013607.2"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="low molecular weight phosphatase family protein"
                     /protein_id="HOQ52880.1"
                     /translation="MTTDDVTAHPSVLFVCVKNGGKSQMAAGLMRKAAGDAVIVGSAG
                     TRPGAVINGLSARVLLEVGVDITDQTPQQLTDDLIAAADRVIVLGREAQVRSVDGTPV
                     EYWDTDEPSERGIDGIDRMRLVRDDISRRVHDLAGDLAAPNSGMAPHLDGRQ"
     gene            complement(3495..3911)
                     /locus_tag="PLF56_04635"
     CDS             complement(3495..3911)
                     /locus_tag="PLF56_04635"
                     /EC_number="1.20.4.4"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_020575156.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_function: GO:0030612 - arsenate reductase (thioredoxin)
                     activity [Evidence IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="arsenate reductase ArsC"
                     /protein_id="HOQ52881.1"
                     /translation="MSDKPSVLFVCVHNAGRSQMAAGFLAALSDGRVEVRSAGSMPAG
                     PVNPVAVQAMQEVGVDIAGQQPKIQTTDAVQASDVVITMGCGDACPIFPGKRYEDWKL
                     DDPAGQGIESVRPIRDDIRVRVQTLLTELLPTGSQT"
     gene            4140..5165
                     /gene="trxB"
                     /locus_tag="PLF56_04640"
     CDS             4140..5165
                     /gene="trxB"
                     /locus_tag="PLF56_04640"
                     /EC_number="1.8.1.9"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_014848078.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_function: GO:0004791 - thioredoxin-disulfide reductase
                     activity [Evidence IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="thioredoxin-disulfide reductase"
                     /protein_id="HOQ52882.1"
                     /translation="MHDVIVVGSGPAGYTAAIYLARAELKPLVFEGYTYGGALMNTTE
                     VENFPGFPAGIMGPELMAQIREQAERFGADLRTVDAEAVDLSGPIKTVTVDGEVHRAR
                     AVVLATGSAARYIGLPDEQRLVGRGVSACATCDGFFFRDQDILVVGGGDSAMEEATFL
                     TRFARSVTIVHRREEFRASAIMIERARQDPKIKWLTNVVVSALHGETSLTGVTVTDTR
                     TGEQTEVAATGLFVAIGHDPRSELFRDQVTVDHEGYVLAGHHVSAIANTGTSVEGVFA
                     CGDLVDHTYRQAITAAGSGCAAALDAQHYLAAHPAPAGAEVIGAAHPNDPMPETPQAA
                     FSDALKV"
     gene            complement(5274..5777)
                     /locus_tag="PLF56_04645"
     CDS             complement(5274..5777)
                     /locus_tag="PLF56_04645"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_020575166.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="DinB family protein"
                     /protein_id="HOQ52883.1"
                     /translation="MNRSDIQAELRRAQTDFHDLVARATPQDLRRRTDGTRWTNRQLL
                     WHMVFGYLIVRTLLPLVHTLGRLGRSRRFAATLDVAQRPFHWINYAGSAGGGLLLTPH
                     RMAAIMDATINALGRKLDEETPTALALTMHFPTRWDPYFQPTMSVLDVYHYGTQHYDH
                     HRRQLTL"
     gene            5969..>7054
                     /locus_tag="PLF56_04650"
     CDS             5969..>7054
                     /locus_tag="PLF56_04650"
                     /inference="COORDINATES: protein motif:HMM:NF019604.2"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="NAD(P)-binding domain-containing protein"
                     /protein_id="HOQ52884.1"
                     /translation="MGAGQRVAAGSSAVGSAVASAGLPVVVIGGGPVGLAAAANLLER
                     GLEPLVLEAGDRVGSAISEWGHVSLFSPWAYTVDAASRRLLNRTAWRQPDPKALPTGR
                     DLVEQYLVPLAATAELVGRIRTGTRVVAVTRQGVDKTRSIGRDGRPYVVRVLTSDGVT
                     DIAAAAVIDASGTWGRPNPLGVAGLPADGESEAAPWLAGPLPDVLGRDRARFAGRRVL
                     VVGMGHSAANTLLALVRLQRQEPATRVTWAIRAASPARLYGGRKADQLPARGALGGGV
                     RAAVESGAITLLSETTIDSFRPGGDGGLRVGLTSQGRREAVEVDVVVGATGFRPDLDM
                     LREVRLDLDPALEAPAELAPLIDPNFHS"
BASE COUNT         1033 a         2324 c         2579 g         1118 t
ORIGIN      
        1 cgctacgagt gggcccgcac ccgggtgcac ggcatcaact accccgacgc ggtgtactgg
       61 ctgatggctc gccggagcct ggcggatccg accgacctgg cctactacct gtgccacgcg
      121 ccggcccggg ccagcctggc cgacctggtc aaagtggccg gaatccgttg gaccatcgag
      181 gaaaccttcc agaccgcgaa aggccagacc gggctggacc actaccaggt ccggcagtac
      241 accggctggt accggcacat caccttgtcc atgctcgccc acgcctacct gaccgtgacc
      301 cggtcaaaaa ggggggccca ccaaacggca ccgacggcct ggtcgacctc agcacccccg
      361 agatccggcg actcctcaac cacctgatct ggaccggaca gcccgaccaa gaccacgtcc
      421 tacgccggtc acgatggcgc cgacgccacc aacaccgagc ccgccaatgc cactaccgca
      481 cccgcggaca ccttccataa gtgcgactgt agtactaggc ggcggtcgcg gcgggccggt
      541 cacagctgca gccggctccg cagcccaccg gccggcggcg acggcgtgcg gtgacccaga
      601 acaggcccgc ggcggccgcg gcgaggacga gcgcgatcgt gggcatccac cgacccaggg
      661 tgatccaggt ggcaccgccg atcactccgg cggtcagcag gaccgggatc agacagcagg
      721 ccaggcaagc gagtccggcg atgccggcca gcgccctcga tatcgcggat ccggaggtcg
      781 cccgtgggtg atcgtcgggc ggcggcatca gactggctcc gggcgggtgg tggtcagctc
      841 ggtgaacggc agcgggcagc aggagctgcc ggcgcaggtg acgaggtcgt cgcagccggc
      901 gtcgagggcc tggaccaggg attcgcggat gacggtcagg tccgcgatct tccggtcgat
      961 ctcggcgacc ttggccgccg ccctggcctg caggccggcg tcacggcggc cgtgatggtg
     1021 gctgccggtt tccaggagct cggcgacctc gctgagggag aaccccagcc gctgcgccgt
     1081 tttgatgacc cggagcaggg tgatcgcctg gtccggatag atccggtgcc cgcccagggt
     1141 gcggtccggt tcgtcgagca gaccgcgacg ttcgtagtac cgcagggtct gcaggttcac
     1201 cccggcagcg gccgcgacct gcccggcacg cagtcccgcg ctcaccgggc taccccgcgg
     1261 gcccggccga ccagggcgtc caggacctcg atctgtgcgg tgggggtgcg gatctgcagg
     1321 gtcaggtgcc caccggtcgc ggtgagggtg aattcgaaga acgagcagca ggcggtctcc
     1381 cgcacggcca ggtcggccgc acggccggca accgtggggt ccggtcgcag ggtcagaatg
     1441 gcggtgtcgg ccgagggctg ctgcacgtcg gtgacgtccg cggtgaacag ggcgtcgaac
     1501 tcggcgagcc gcaggggctg ctgcgcggtg ggcagggtgc acgcttccgg cacccaggtg
     1561 ggttcggtct gtgtcatgta ctcgacggta agcccgtacc taggtaacag atgcaagccg
     1621 acatcggcgc gttcgctaat tttgctggcg accatgccac ggggacggaa gaaacgaagg
     1681 acgtcccaaa tcgttcggat ctgctcgacc gcggccgcgg ggcccggagc agctgaacgt
     1741 gagcggagac cagcggcgaa actctgtagc ggattcaacg acaaggatct gccgagcgct
     1801 gacgatttct gcagcattcg gcagtggccg atcgtgatcg tcccagttca tttggacctg
     1861 gacggcggcc gcttggccac tgaggggatc cccaggccct gaagtgtcca gacggcgcga
     1921 tcgcgcatgc tcagccgggc agccggatgt tgcgaccact atcgagtgcc tctgtcccag
     1981 accttggccg gctgaagctg ggtagtccgc gaacgctcgt tcatgaagcg tagatgcggg
     2041 gcccggcacc tcccttgggt caggtccctg gtctgagctt ccgccgggat gcgcggcacc
     2101 agtggaccgg tggcccgccc gtcggggaaa tccgtcgccg cgggaagctg aggggaaccg
     2161 agtgttggga agccggtgac gggattcgcg tggttcaccg cgcgggcctg gaacacgatc
     2221 gacccataga gtgaccggaa tgaccgcctc gccgcccggg cgtagttcgg tcgccgccgc
     2281 cgggatgatc cggcggtcgc tgccgctggc gttcctgacc ggcgccggta tcgggatcct
     2341 cggcggcatg atcggcctgg gcggcgcgga gttccgactg cccctgctga tcggggtgtt
     2401 cgggttcctg gccctgcagg cggtgatcct gaacaggcga tgagcctgat cgtggtcctg
     2461 accgcggtgc cggcccggct ggtctccgtc cccttcggcg acctggcccc gtattggtac
     2521 gtcgtggtca acctgctggc gggcagcctg gtcggggcct ggatcggcgc gacctgggcc
     2581 accagaatgc ggtcggccac cctctacagg gtcatcgccg tgctcctggt gttcatcgcg
     2641 gccgcgctgg cgaccaccca cctcgcgcag gtcggcacgc tggacctgcc acccgtgccg
     2701 cggaccatcc tcggtgtcgc tgccggggtc gggatcggcg tggtcgcggc ggtcatgggc
     2761 gtcgccggcg gcgagctgct catcccgacc atcgtgttgc tctacggcgt cgacatcaag
     2821 atcgccggca gcctgtcgct ggtggtctcc ctaccgacaa tgctggtcgc gttcgcccgt
     2881 tactcccgcg acaacagctt ccaggccctg cgcgacaaca aggccttcgt cgtcgccatg
     2941 accatcggat ccatcgccgg aacaatcgtc ggcgggctgc tgctcggggc cgtcccgacc
     3001 ctggtcctca ttccactact ggtggtgctg ctcctactgt cggccgtcaa ggtgtggcgc
     3061 catacctgag ttcggggcgg ccagatcgcc ggcgaggtcg tggacccgcc gactgatgtc
     3121 gtcgcggacc aggcgcatcc ggtcgatccc gtcgatgccc cgttcggacg gttcgtcggt
     3181 gtcccagtac tccacgggag tgccgtcgac ggaccggacc tgagcttccc ggcccaggac
     3241 gatgacccgg tcggccgccg cgatcaggtc gtcggtcagt tgctggggag tctggtcggt
     3301 gatgtccacg ccgacctcga gcagaacccg cgcagacagg ccgttgatca ccgcaccagg
     3361 ccgggttccg gccgagccga cgatgacggc gtcgccggcg gccttgcgca tgagaccggc
     3421 ggccatctgg gacttgccgc cgttcttcac gcacacgaac agcaccgacg gatgggccgt
     3481 gacgtcgtcg gtggtcatgt ctggctgccg gtgggcagga gttcggtgag cagggtttgc
     3541 acccggacgc ggatgtcgtc gcggatgggg cggacggact cgatgccctg gccggcgggg
     3601 tcgtcgagtt tccagtcctc gtaacgtttc ccggggaaga tcgggcaggc gtcgccgcag
     3661 cccatggtga tgaccacgtc ggatgcctgc accgcgtcag tggtctggat cttgggctgc
     3721 tggccggcga tatcgacgcc gacttcctgc atggcctgga cggcgaccgg gttgaccggg
     3781 cccgcgggca tcgacccggc cgaccgcacc tcgacccggc cgtcggacag cgcggctagg
     3841 aaaccggcgg ccatctggga ccggccggcg ttgtgcacgc acacgaacaa cacgctgggc
     3901 ttgtcggaca ccgcggaacc tccatcggat caagatcacc ggccggccgt gacttgacca
     3961 ggcgcatagc tcaatgatac ttgagacaat acgcattgag tgaaccacga aaaagcgtga
     4021 ccgggcccgg atgctgcatg aggagagatg tcgtgaatca gttcgtgtcc attccgggga
     4081 tccaggccgg atcgaacccg gaaccgaccg ccgccgcggc ggattgcgct ggcgaccgcg
     4141 tgcacgacgt gatcgtcgtc gggtcgggtc cggccggcta caccgccgcg atctacctgg
     4201 cccgggccga gctcaagccg ctggtcttcg agggctacac gtacggcggg gccctgatga
     4261 acaccaccga ggtggagaat tttccgggct tcccggccgg gatcatgggc ccggagttga
     4321 tggcccagat ccgcgaacag gccgagcggt tcggtgccga cctgcgcacc gtggatgcgg
     4381 aggccgtcga cctgtccggg ccgatcaaga cggtcaccgt ggacggtgag gtgcaccgcg
     4441 cccgggcggt cgtgctggcc accggatcgg cggcccggta catcggcctg cccgacgagc
     4501 agcggctggt cggtcgcggc gtgtccgcct gcgccacctg cgacggcttc ttcttccgcg
     4561 accaggacat tctggtcgtc ggcggtggcg actcggccat ggaggaggcg accttcctca
     4621 cccgattcgc tcggtcggtg acgatcgtgc accggcgcga ggagttccgc gcgagcgcca
     4681 tcatgatcga acgggcccgt caggacccga agatcaagtg gctgaccaac gtcgtggtca
     4741 gcgccctgca tggcgagacc agcctgaccg gggtgaccgt caccgacacc cgcaccggcg
     4801 agcagaccga ggtggcggcg accggtttgt tcgtcgcgat cggccacgac ccgcggtccg
     4861 aactgttccg cgaccaggtg accgtcgacc acgagggcta cgtcctggcg ggccaccacg
     4921 tgtcggcgat cgcgaacacc gggaccagtg tggagggcgt cttcgcctgc ggcgacctgg
     4981 tcgaccacac ctaccggcag gcgatcaccg cggcgggcag cgggtgcgcc gccgccctcg
     5041 acgcccagca ctacctggcc gcccacccgg caccggccgg ggccgaggtg atcggcgccg
     5101 cgcacccgaa cgacccgatg cccgagaccc cgcaagccgc gttctcggac gcactgaagg
     5161 tctgagtagt agtccgggac tctaggcggc caacttaggg acggcccgct ggttccacgg
     5221 tcggtgggct gcaagtgcct gtgaatactc cccgcgcgca acaacttcac cgctcacagg
     5281 gtcagctggc gacggtggtg gtcgtagtgt tgggtgccgt agtggtagac gtccagcacg
     5341 ctcatcgtgg gttggaagta ggggtcccag cgggtcggga agtgcatggt gagcgcgagg
     5401 gccgtgggtg tttcctcatc cagcttgcgg cccagggcgt tgatggtggc gtccatgatg
     5461 gccgccatgc ggtgcggggt gagcagcagc ccgccgccgg cggagccggc gtagttgatc
     5521 cagtggaacg gccgctgggc gacgtcgagg gtggccgcga atcgccggct gcgtcccagc
     5581 cgtcccagcg tgtggaccaa cggcagcagg gtgcgcacga tgaggtaccc gaacaccatg
     5641 tgccacagca gctgtcggtt ggtccaacgg gtgccgtcgg ttcgccggcg caggtcctgc
     5701 ggggtggccc gggccacgag gtcgtggaag tcggtctggg cgcgtcgtag ttcggcctgg
     5761 atgtcgctgc ggttcatggc cggatctccg ttcgagtcgg ctgtcccagt gtctcgcgtg
     5821 gggttgcatg gcgggtcacc cgagggcgtc aggggtcggg ccatcacgag gatcggggat
     5881 gacatccggg caatacattg acaaacgtcg atgtatcgac aatcctctat acatcgagga
     5941 gcgtcgatgt atggaaggga tggttccgat gggtgcaggg cagcgggtgg cggcgggttc
     6001 ctcggcggtg ggatcggcgg tcgcgtcggc gggcttgccg gtggtggtga tcggtggtgg
     6061 tccggtcggg ttggcggcgg ccgcgaacct gttggagcgg gggctggagc cgttggtgtt
     6121 ggaggccggc gatcgggtcg ggtcggcgat ctcggagtgg ggtcacgtgt cgctgttctc
     6181 tccgtgggcc tacaccgtcg acgccgcctc gcggcggctg ttgaaccgga ccgcctggcg
     6241 tcagccggat ccgaaggcgt tgcccacggg acgcgatctg gtggagcagt atctggtgcc
     6301 gttggcggcc acggcggagt tggtcggccg gatccgcacc ggtacccggg tggtcgcggt
     6361 gacccggcag ggggtggaca agacccgcag catcggccgg gacgggcggc cctacgtggt
     6421 gcgggtgctg acctcggacg gggtcaccga catcgcggcg gccgcggtca tcgacgcctc
     6481 cggcacctgg ggtcggccga atccgctcgg cgtggccggc ctgccggcgg acggcgaatc
     6541 cgaggccgca ccctggctgg ccgggccgtt gccggacgtg ctgggccggg accgggcccg
     6601 gttcgccggc cgccgggtgc tggtcgtcgg gatggggcac tcggccgcca acacgctgct
     6661 ggcgttggtg cggttgcagc ggcaggaacc ggccacccgg gtcacctggg cgatccgggc
     6721 ggcgtccccg gcccggctct acgggggtcg caaggccgac cagttgccgg cccgcggcgc
     6781 cctgggtggc ggggtgcggg cggccgtcga gtccggtgcg atcaccctgc tgtccgagac
     6841 caccatcgac agtttccggc ccggcgggga cggcggcctg cgggtcggcc tgaccagcca
     6901 gggtcgccgc gaagcggtcg aggtcgatgt ggtggtcggc gcgaccggtt tccgcccgga
     6961 cctggacatg ctgcgggagg tgcggctgga tctggatccc gccctggagg cgccggccga
     7021 gctggcgccg ctgatcgacc cgaacttcca ctcc
//
LOCUS       DAOSUG010000097         7052 bp    DNA     linear   ENV 10-JUN-2024
DEFINITION  MAG TPA_asm: Micropruina sp. isolate AMR_MDS_2195
            NODE_5213_length_7052_cov_4.344433, whole genome shotgun sequence.
ACCESSION   DAOSUG010000097 DAOSUG010000000
VERSION     DAOSUG010000097.1
DBLINK      BioProject: PRJNA925477
            BioSample: SAMN32864262
            Sequence Read Archive: SRR4236673
KEYWORDS    WGS; ENV; Metagenome Assembled Genome; MAG; Third Party Data; TPA;
            TPA:assembly.
SOURCE      Micropruina sp. (wastewater metagenome)
  ORGANISM  Micropruina sp.
            Bacteria; Bacillati; Actinomycetota; Actinomycetes;
            Propionibacteriales; Nocardioidaceae; Micropruina.
REFERENCE   1  (bases 1 to 7052)
  AUTHORS   Abdulkadir,N., Saraiva,J.P., Zhang,J., Stolte,S., Gillor,O.,
            Harms,H. and Rocha,U.
  TITLE     Genome-centric analyses of 165 metagenomes show that mobile genetic
            elements are crucial for the transmission of antimicrobial
            resistance genes to pathogens in activated sludge and wastewater
  JOURNAL   Microbiol Spectr 12 (3), e0291823 (2024)
   PUBMED   38289113
REFERENCE   2  (bases 1 to 7052)
  AUTHORS   Abdulkadir,N.
  TITLE     Direct Submission
  JOURNAL   Submitted (24-MAR-2023) Environmental Microbiology, Helmholtz
            Center for Environmental Research -UFZ, Permoserstr 15, Leipzig
            04318, Germany
COMMENT     The annotation was added by the NCBI Prokaryotic Genome Annotation
            Pipeline (PGAP). Information about PGAP can be found here:
            https://www.ncbi.nlm.nih.gov/genome/annotation_prok/
            
            ##Genome-Assembly-Data-START##
            Assembly Method        :: SPAdes v. 3.11.1
            Genome Representation  :: Full
            Expected Final Version :: Yes
            Genome Coverage        :: Not Applicable
            Sequencing Technology  :: Illumina MiSeq
            ##Genome-Assembly-Data-END##
            
            ##Genome-Annotation-Data-START##
            Annotation Provider               :: NCBI
            Annotation Date                   :: 05/04/2023 04:57:10
            Annotation Pipeline               :: NCBI Prokaryotic Genome
                                                 Annotation Pipeline (PGAP)
            Annotation Method                 :: Best-placed reference protein
                                                 set; GeneMarkS-2+
            Annotation Software revision      :: 6.5
            Features Annotated                :: Gene; CDS; rRNA; tRNA; ncRNA
            Genes (total)                     :: 2,776
            CDSs (total)                      :: 2,750
            Genes (coding)                    :: 2,714
            CDSs (with protein)               :: 2,714
            Genes (RNA)                       :: 26
            tRNAs                             :: 24
            ncRNAs                            :: 2
            Pseudo Genes (total)              :: 36
            CDSs (without protein)            :: 36
            Pseudo Genes (ambiguous residues) :: 0 of 36
            Pseudo Genes (frameshifted)       :: 2 of 36
            Pseudo Genes (incomplete)         :: 32 of 36
            Pseudo Genes (internal stop)      :: 2 of 36
            ##Genome-Annotation-Data-END##
FEATURES             Location/Qualifiers
     source          1..7052
                     /organism="Micropruina sp."
                     /mol_type="genomic DNA"
                     /submitter_seqid="NODE_5213_length_7052_cov_4.344433"
                     /isolate="AMR_MDS_2195"
                     /isolation_source="Activated sludge of municipal
                     wastewater treatment plants"
                     /db_xref="taxon:2737536"
                     /environmental_sample
                     /geo_loc_name="Canada"
                     /lat_lon="49.25 N 123.1 W"
                     /collection_date="2015-06-16"
                     /metagenome_source="wastewater metagenome"
                     /note="metagenomic"
     gene            445..1488
                     /gene="cobT"
                     /locus_tag="PLF56_04655"
     CDS             445..1488
                     /gene="cobT"
                     /locus_tag="PLF56_04655"
                     /EC_number="2.4.2.21"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_011755957.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_function: GO:0008939 -
                     nicotinate-nucleotide-dimethylbenzimidazole
                     phosphoribosyltransferase activity [Evidence IEA];
                     GO_process: GO:0009236 - cobalamin biosynthetic process
                     [Evidence IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="nicotinate-nucleotide--dimethylbenzimidazole
                     phosphoribosyltransferase"
                     /protein_id="HOQ52885.1"
                     /translation="MNITAPGTAVQAPSNAAYAAARALSDGQAKPLGALGDLETLAAW
                     VASCQGVCPPRQLDDVRVVVFAGDHGIATDGVSAYPVEITPAMVYGIAGGHAGVSALA
                     AANDVRVSIYDLGVAVDFPDLPAEVSRFKVRRSSGAIHLTDALTTNEVEAALAAGDRI
                     AAEQIADGAQLLIAGDLGIGNTTIAAALIAASLGVPAAAVTGRGTGIDDATLVHKTAL
                     VEAALARAGERVADPAQRLATLGAADFAAAAGFIAGAARRGVPVLLDGVIAVAEALVA
                     EDLAPGTKAWLQAGHRSTEPGQAIALDRLGLKPLVDFSMRLGEGSGAVLAVPMVRSAV
                     AALNGIAQLSDLA"
     gene            complement(1669..2847)
                     /locus_tag="PLF56_04660"
     CDS             complement(1669..2847)
                     /locus_tag="PLF56_04660"
                     /EC_number="3.1.1.103"
                     /inference="COORDINATES: protein motif:HMM:NF012371.2"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_function: GO:0016787 - hydrolase activity [Evidence
                     IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="serine hydrolase domain-containing protein"
                     /protein_id="HOQ52886.1"
                     /translation="MSTTDVTVTDPSAVGVDEAALARLDAAIQRDIDAKLNYGASIIV
                     ARGGRIAHRKTFGTVGPDRAAADDDRYLLMSMSKGYVAALTLKAIDEGRFGLDTRVAD
                     LVPGFGVAGKQRATVRHLLTHTAGLPFALLPPGLPLDKVGDLTAKTRVICGLSAEYIP
                     GTRCVYTSAVGYDLLGQLLVNTDPAGRSFRSIVSEDLFAPLGMNDSSFGVDVDDPRRV
                     LASATPENSTPMSPLLVNACNTYFTGEAEFPAGNAFSTIDDVYRFTQLYRGRGTAGGQ
                     RLVSPALFDYADRNHTGDLTNDGWIFETEPRGMDSFPAHFSLLGGYVRGEGHVFNPAG
                     LTASPRTIACVGGGSTSFIVDPERDLTFIFLSAGFFTGLPHLERVSRLNDLALATVND
                     "
     gene            2939..3541
                     /locus_tag="PLF56_04665"
     CDS             2939..3541
                     /locus_tag="PLF56_04665"
                     /inference="COORDINATES: protein motif:HMM:NF028169.2"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_function: GO:0003677 - DNA binding [Evidence IEA];
                     GO_function: GO:0003700 - DNA-binding transcription factor
                     activity [Evidence IEA];
                     GO_process: GO:0006355 - regulation of DNA-templated
                     transcription [Evidence IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="TetR/AcrR family transcriptional regulator"
                     /protein_id="HOQ52887.1"
                     /translation="MVASARRARRTNVQVTAAITTAVLDELREVGFAGLTFESVARRA
                     ELSKAVLYRRHPTRVSLALAGLQEAAQGIYAEPPMTGSLRTDLLAWLAAADAQVPPSG
                     AATFRGLVGEATPTELEHVAVLMGTRADDIERCIIEPARQRGEIAGDIPAPVAKLLFI
                     LLRDQTLFEPESDHSHTAIVDHILLPLLTANPSPAQPDQH"
     gene            3573..4244
                     /locus_tag="PLF56_04670"
     CDS             3573..4244
                     /locus_tag="PLF56_04670"
                     /inference="COORDINATES: protein motif:HMM:NF025160.2"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="HXXEE domain-containing protein"
                     /protein_id="HOQ52888.1"
                     /translation="MTTSSESFLVKLSGQWWINVVYGLGAIALVATLVHGSTWEPPRL
                     VAALFAAGLALHVLEELNYPAGFHYMLNSIQNSEQPEVGPENRLSDLITNLGVQLLIV
                     AVMIAGGNKATTVAFVLFGIGESIVHLLFGVLILRKLRHKGKRTIYGPGTITALLAFL
                     PAAIYAWAWLTTQTVGAWDIAIGVLIIAFTMGIMIRLPMMLIDGRNHPELAYTSTGYF
                     AKYLK"
     regulatory      4414..4553
                     /regulatory_class="riboswitch"
                     /inference="COORDINATES: nucleotide
                     motif:Rfam:14.4:RF00174"
                     /inference="COORDINATES: profile:INFERNAL:1.1.4"
                     /note="cobalamin riboswitch; Derived by automated
                     computational analysis using gene prediction method:
                     cmsearch."
                     /bound_moiety="adenosylcobalamin"
                     /db_xref="RFAM:RF00174"
     gene            4584..5351
                     /gene="cobI"
                     /locus_tag="PLF56_04675"
     CDS             4584..5351
                     /gene="cobI"
                     /locus_tag="PLF56_04675"
                     /EC_number="2.1.1.130"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_002513151.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_function: GO:0030788 - precorrin-2
                     C20-methyltransferase activity [Evidence IEA];
                     GO_process: GO:0009236 - cobalamin biosynthetic process
                     [Evidence IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="precorrin-2 C(20)-methyltransferase"
                     /protein_id="HOQ52889.1"
                     /translation="MSTPAWPASPTLVGVGVGPGDPSLVTLRAVDVLRRAEVILVPAT
                     DTATSDEGRAEVIVRAVCEGAESRLRRVPFSMARKSGVGERRRQAWQASAEAATDAFA
                     AGARTVAFATVGDPSVYSTFSYLAAHVRAAVPGLVVEVVPGITAMQALAAASRTPLVE
                     GRESLTLVPVTAGLDAVAAALEHSDTVVAYKGGRQLPDLLNLVRERRPDGVGVLGVNV
                     GLPGEQLVPLAEVPDGSAPYFSTVLVAPARTNTGGRL"
     gene            5348..6166
                     /gene="cobM"
                     /locus_tag="PLF56_04680"
     CDS             5348..6166
                     /gene="cobM"
                     /locus_tag="PLF56_04680"
                     /EC_number="2.1.1.133"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_002515099.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_component: GO:0005737 - cytoplasm [Evidence IEA];
                     GO_function: GO:0046026 - precorrin-4
                     C11-methyltransferase activity [Evidence IEA];
                     GO_process: GO:0009236 - cobalamin biosynthetic process
                     [Evidence IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="precorrin-4 C(11)-methyltransferase"
                     /protein_id="HOQ52890.1"
                     /translation="MSGEVVFVGAGPGAADLITVRGARVIAEADIVIWASSLVDEAML
                     AGVRPDALVVDSASLPLEGIEPLYERARDEGLLVARVHTGDPSIYGATAEQRRLCDRI
                     GVAHRTIPGVSAFSAAAARMDVEITVPEVSQTLILTRLEGGRTPMPDRETVRGFAEHG
                     VTMALYLSAARNKVLQAELLAGGYPAETPCVVGYRVSWPDELMLRCRLDELSATMAEH
                     KLWKHTVVLVGPALAENGDTRSHLYHPGFRHEYRKAAGEAPIPRADTNHREVRR"
     gene            6163..>7052
                     /gene="cbiE"
                     /locus_tag="PLF56_04685"
     CDS             6163..>7052
                     /gene="cbiE"
                     /locus_tag="PLF56_04685"
                     /inference="COORDINATES: protein motif:HMM:TIGR02467.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_function: GO:0046025 - precorrin-6Y
                     C5,15-methyltransferase (decarboxylating) activity
                     [Evidence IEA];
                     GO_process: GO:0046140 - corrin biosynthetic process
                     [Evidence IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="precorrin-6y C5,15-methyltransferase
                     (decarboxylating) subunit CbiE"
                     /protein_id="HOQ52891.1"
                     /translation="MITVYGLLGEPSPALRAAAAGATTVVGATRHLDALAVEADRRIT
                     LGALRPAVEQLTRLPPDADVVVVASGDPLFFGVVRSLRARGLRPAVVPAVSSIAAAFA
                     AVGLPWDDAAVVSVHGRPIEAALDLARRAGKVAVFTSPENGVRELAAGLDGLQRWFVL
                     AERLGEADECVRVLDCEQALTVEPRQPNVVLILDRLPAEPDAEWPGVTAGPVPARMRA
                     QTGRPAGADPVIGQVLGGAASVAHADRIDALLGKPTRRYTEGASAGLPQAFAECDLIV
                     SHLALGATTRIIAPLLRDKHT"
BASE COUNT         1218 a         2412 c         2384 g         1038 t
ORIGIN      
        1 ctcacactca aagataaaaa atgggaatac aaaatatatg agaaagagga tgacaaattc
       61 atagaagtat tcagccgtat aagtcccaaa ccacccacag aacagtccca aaatgctaat
      121 atgttcacaa aaccacataa aatcatttat gggacaccag aaaccaagga tgaaaacacc
      181 agcctggtca gcagcacatt cataaaactt gaaaatatcg tactggaaaa gatagagaac
      241 actaaacagg caatattcaa taaaaaagaa aatgaataca gagaactcca gataataggg
      301 gaacaattct acaataaatt taaacaggaa aacaggaatg taatctccta tatagccagt
      361 gtctgcagac taatcagtgc caaagaagaa ggaaacatca ttaaagtcct ccttgcaatg
      421 atttccaccg ctaaaggaga acccatgaac atcaccgcac ccggcaccgc cgtccaggcc
      481 cccagcaacg ccgcctacgc ggcggcccgc gctctcagcg acgggcaggc caagccgctc
      541 ggcgccctcg gcgacctcga aacgctggcc gcctgggtgg cctcctgcca gggcgtctgc
      601 ccgccgcggc aactggacga cgtgcgcgtg gtggtcttcg ccggcgacca cggcatcgcg
      661 actgacgggg tgtcggccta cccggtcgag atcaccccgg cgatggtcta cggcatcgcc
      721 ggcgggcatg ccggggtgtc cgcgctggcg gccgccaacg acgtccgggt ctcgatctac
      781 gacctggggg tcgccgtcga ctttcccgac ctgccggcgg aggtcagcag attcaaggtg
      841 cgccgctcgt ccggggcgat ccacctgacc gacgcgctca ccaccaacga ggtcgaggcc
      901 gccctggccg cgggcgaccg gattgccgcc gagcagatcg cggacggcgc gcagctgctg
      961 atcgccggcg acctcggcat cggcaacacc acgatcgccg ccgccctgat cgccgcgtcg
     1021 ctgggcgtgc ccgccgccgc ggtgaccgga cgcggcaccg gcatcgacga cgccaccctg
     1081 gtgcacaaga ccgcgctggt cgaggcagcg ttggcccggg cgggtgaacg cgtcgccgat
     1141 ccggcgcagc ggttggccac gctgggcgcc gccgacttcg ccgcggccgc agggttcatc
     1201 gccggggcgg cccgacgggg cgtcccggtg ctgctggacg gggtgatcgc ggtcgccgag
     1261 gcactcgtgg ccgaagacct cgcccccggc acgaaggcgt ggctgcaagc cggtcaccgc
     1321 tccaccgaac cgggccaggc gatcgccctc gaccggctcg ggctcaagcc gctggtcgac
     1381 ttctcgatgc gcctgggcga gggcagcggc gcggtgctcg cggtcccgat ggtgcgctcc
     1441 gcggtggccg cgctgaacgg catcgcgcaa ctgagcgacc tggcctgaac gcagggccgc
     1501 caaggcactt cgattgaccc gatcaagaag gcgcagcacg gtgtgggtgg cgttgcggct
     1561 cgcgccgatg aaggacgcag acctggtgtg acgggtgggg agtgcactcg agttggtgcc
     1621 agaccagcgc cggatgaggt agctgatcat ggctccgctc ctggcgggtc agtcgttgac
     1681 ggtggccagg gccaggtcgt tgaggcggct gacgcgttcg aggtggggca gcccggtgaa
     1741 gaaccctgcc gacaagaaga tgaaggtgag gtcgcgttcg gggtcgacga tgaaggaggt
     1801 cgagcctccg ccgacgcagg cgatggtccg gggcgaagcg gtgagaccgg cggggttgaa
     1861 gacgtggccc tcaccgcgga cgtagccacc cagcagggag aaatgggcgg ggaaggagtc
     1921 catgccgcgg ggctcggtct cgaagatcca gccatcgttg gtgaggtcgc cggtgtggtt
     1981 gcggtcggcg tagtcgaaca gggccgggga caccaaacgc tggccgccgg cggttccacg
     2041 accgcggtac agctgtgtga agcggtacac gtcatcgatc gtggagaagg cgttcccggc
     2101 ggggaactct gcctcgccgg tgaagtaggt gttgcaggcg ttgaccagca gcgggctcat
     2161 cggggtcgag ttctccggcg tggcggaagc gagcactcga cgcggatcgt cgacgtcgac
     2221 accgaagctg ctgtcgttca tacccagcgg cgcgaacagg tcttcggaga cgatgctgcg
     2281 gaaggaccgt ccggcggggt cggtgttgac cagcagctgg ccgagcaggt cgtagccgac
     2341 cgccgaggtg tagacgcagc gcgtcccggg aatgtactcc gccgacaggc cgcagatcac
     2401 gcgggtcttc gcggtgaggt cgccgacctt gtccaggggc aggccgggag gcagcagggc
     2461 gaacggcagc ccggcggtgt gggtcaggag gtggcggacg gtggcgcgct gcttgccggc
     2521 cacaccgaac ccgggaacca gatcggcaac ccgggtatcg agaccgaagc ggccctcatc
     2581 gatcgccttc agggtcaacg cggcaacata gcccttgctc atcgacatca gcaggtatcg
     2641 gtcgtcgtcg gccgcggcac ggtcgggacc cactgtgccg aaggtcttgc ggtgggcgat
     2701 gcggccaccg cgggccacga tgatgctggc cccgtagttc agcttggcgt cgatatcgcg
     2761 ctggatcgcc gcgtcgagac gggcgagtgc cgcctcgtcc acgccgacag cggacgggtc
     2821 ggtgacggtc acatcggtgg tgctcatctc ttgctccttt taggtacggt acgtgcttat
     2881 agccagcata ggctacgatt tttgagtacg caaggtatct aaacaggagg ggcgactgat
     2941 ggtggcatcg gcacggaggg cccgccggac caacgtccag gtcacggccg cgatcactac
     3001 cgccgtgctg gacgaactcc gcgaggtcgg gttcgcaggc ctgaccttcg agagcgtcgc
     3061 gcgacgtgcc gaactgagta aggccgtgct ctaccgacgc caccccaccc gggtatccct
     3121 cgccctggcc ggactccagg aagctgcgca gggcatctat gccgaacccc ccatgaccgg
     3181 ctcactgcgc acagacctgc tggcctggct cgccgccgcc gacgcccaag tgccgcccag
     3241 cggggcggcc acctttcgcg gcttggtcgg cgaagccacc ccgaccgaac tcgaacacgt
     3301 cgccgtactg atgggcaccc gcgccgacga catcgaacgc tgcatcatcg aaccagcccg
     3361 gcaacgaggc gagatcgccg gcgacatccc cgcccccgtc gccaaactcc tgttcatcct
     3421 gctccgggac caaaccctgt tcgaacccga atccgaccac tcccacaccg ccatcgtcga
     3481 ccacatcctg ctcccgctac tcaccgccaa cccgagcccc gcccagccag accagcactg
     3541 aaacgacccg acacccccga aggagaaccg gcatgaccac aagctcggaa tcattcctgg
     3601 tgaaactgtc cgggcagtgg tggatcaacg tcgtctacgg cctcggcgcg atcgcactcg
     3661 tcgcgaccct ggtccatggc tccacatggg aaccgccacg cctggtcgct gccctgttcg
     3721 ccgcaggcct cgccctgcac gtactggagg aactcaacta cccggccggt ttccactaca
     3781 tgctcaacag catccaaaac tccgaacaac ccgaagtcgg accagaaaac aggctctccg
     3841 acctgatcac caacctcgga gtccaactgc tcatcgtcgc cgtcatgatc gccggcggca
     3901 acaaggcgac caccgttgcc ttcgtcctgt tcggcatcgg cgaatcgatc gtccacctcc
     3961 tgttcggcgt cctcatcctg cgaaagctcc gacacaaggg caaacgaacc atctacggcc
     4021 ccggaaccat caccgcgctc ctcgccttcc tgcccgccgc catctacgcg tgggcatggc
     4081 tgaccaccca aaccgtcggc gcatgggaca tcgccatcgg cgtcctcatc atcgccttca
     4141 ccatgggcat catgatccgg ctacccatga tgctcatcga cggacgcaac caccccgaac
     4201 tcgcctacac atcaaccggc tacttcgcca aatacctcaa gtaggcaatg ctcctcgagc
     4261 cggagagacg gcatgcatga ggcacagtcc ttcccggcga tgggtgttgg ggcgtccgat
     4321 ctccgtagtt tcagccctgt cgggcgcgct cacatcgaga ccgcgagcct gaactcagac
     4381 ccgcgagttc accgattccc cggcgcgcgg ccgaccgctc tgcgaggctg aactcacgaa
     4441 tgcagcgtgt cgaggaagtc cggtgagccc tttgcggcaa atccggcgcg gtcccgccac
     4501 tgtgaccgga gcgatccggc agccagacac tcggcgctgc atgcgagcga actgatcggg
     4561 cgtggacacc tgaggaggct cctatgagca cccctgcttg gccggcttcg cccaccctcg
     4621 tcggggtggg cgtgggacct ggcgaccctt ccctggtcac cctgcgtgcg gtggacgtgc
     4681 tgcgccgcgc cgaggtgatc ctggtgccgg ccaccgacac cgcaacctcc gacgagggac
     4741 gcgccgaggt gatcgtccgg gcggtctgcg agggagccga aagccggctg cggcgggtgc
     4801 cgttcagcat ggcgcgcaag agcggggtcg gtgagcggcg ccggcaggcc tggcaggcct
     4861 ccgccgaggc ggccaccgac gcgttcgcgg cgggagcgcg cacggtggca ttcgccacgg
     4921 tgggcgaccc cagcgtgtac tccaccttca gttacctggc cgcgcacgtg cgggccgccg
     4981 tcccaggcct ggtggtcgaa gtggtgcccg gcatcaccgc catgcaggcc ctggccgcgg
     5041 cgagccggac gccactggtc gagggccgcg agtcgctcac cctggtgccg gtcaccgccg
     5101 gcctcgacgc ggttgccgcg gcactcgagc actcggacac cgtggtggcc tacaagggtg
     5161 gacggcagct gcccgacctg ctgaacctgg tgcgcgagcg gcgtccggac ggggtgggcg
     5221 tgctgggcgt gaacgtcgga ctgcccggcg agcagctggt gccgctggcc gaagtgccgg
     5281 acggctcggc gccctacttc tcgacggtgc tggttgctcc ggcccgcacg aacacagggg
     5341 ggcggctgtg agcggcgagg tggtgttcgt cggggccggg cccggcgcgg ccgacctgat
     5401 cacggtgcgg ggagcccggg tgatcgccga ggccgacatc gtgatctggg cgtccagcct
     5461 ggtcgacgag gccatgctgg ccggggtcag gccggacgcg ctcgtggtcg actcggccag
     5521 cctgccgttg gagggcatcg aaccgctgta cgaacgggcc cgtgacgagg gactgctggt
     5581 cgcccgggtg cacaccggcg atccgtccat ctacggcgcc accgccgagc agcgccggct
     5641 gtgcgaccgc atcggcgtcg cccatcggac gattcccggg gtgtcggcct tctcggccgc
     5701 cgccgctcgg atggacgtcg agatcaccgt gcccgaggtc tcgcagacgc tgatcctgac
     5761 ccggctggag ggcggccgca cgccgatgcc cgaccgcgag accgtccgcg gcttcgccga
     5821 gcacggggtg accatggcgc tgtacctgtc ggcggcccgc aacaaggtgc tgcaggccga
     5881 gctgctggcg ggcggctacc cggccgagac cccctgcgtg gtcggctacc gggtcagctg
     5941 gcccgacgag ctgatgctgc gctgccggct ggacgaactg tcggcgacga tggccgagca
     6001 caagctgtgg aagcacacgg tggtgctggt cggcccggca ctggccgaga acggcgacac
     6061 ccgcagccac ctgtatcacc caggcttccg gcacgaatac cgcaaggcag cgggtgaggc
     6121 gccgattccg cgcgccgaca ccaaccatcg cgaggtgcgc cgatgatcac cgtgtacggc
     6181 ctgctcggcg agccctcccc ggccttgcgc gccgccgcgg ccggcgccac gacggtggtc
     6241 ggggccaccc ggcacctgga cgcgctggcg gtcgaggctg atcgccggat caccctgggc
     6301 gcgctgcgtc cggccgtcga gcagctcacc cggctgcccc ccgacgcgga cgtggtcgtg
     6361 gtcgcctcgg gcgacccgct gttcttcggc gtggtgcgca gcctgcgggc ccgaggcctg
     6421 cgacccgcgg tggtgcccgc ggtctcgtcg atcgccgccg cgttcgccgc ggtcggcctg
     6481 ccctgggacg acgccgccgt ggtcagcgtg cacgggcgtc cgatcgaggc ggcgctcgac
     6541 ctggcccggc gggccggcaa ggtggcggtg ttcacctcgc ccgagaacgg ggtgcgcgaa
     6601 ctggccgcgg gactggacgg gctgcagcgc tggttcgtgc tggccgaacg cctcggtgag
     6661 gccgacgagt gcgtccgggt gctcgactgc gagcaggcgc tgaccgtcga accacggcag
     6721 cccaacgtgg tgttgatcct cgaccgacta cctgccgagc cggacgccga atggcccggg
     6781 gtgacggccg gcccggtccc ggcgcgcatg cgtgcacaga cggggcgtcc ggccggcgcc
     6841 gatccggtga tcggacaggt gctcggcggt gcggccagcg tggcgcacgc cgaccgcatc
     6901 gacgcgctac tgggcaagcc gacccgccgc tacaccgagg gcgcgagcgc cgggttgccg
     6961 caggcgttcg ccgagtgcga cctgatcgtc agccacctgg ccctgggcgc caccacccgg
     7021 atcatcgcgc cgctgctgcg cgacaagcac ac
//
LOCUS       DAOSUG010000098         6947 bp    DNA     linear   ENV 10-JUN-2024
DEFINITION  MAG TPA_asm: Micropruina sp. isolate AMR_MDS_2195
            NODE_5304_length_6947_cov_4.097504, whole genome shotgun sequence.
ACCESSION   DAOSUG010000098 DAOSUG010000000
VERSION     DAOSUG010000098.1
DBLINK      BioProject: PRJNA925477
            BioSample: SAMN32864262
            Sequence Read Archive: SRR4236673
KEYWORDS    WGS; ENV; Metagenome Assembled Genome; MAG; Third Party Data; TPA;
            TPA:assembly.
SOURCE      Micropruina sp. (wastewater metagenome)
  ORGANISM  Micropruina sp.
            Bacteria; Bacillati; Actinomycetota; Actinomycetes;
            Propionibacteriales; Nocardioidaceae; Micropruina.
REFERENCE   1  (bases 1 to 6947)
  AUTHORS   Abdulkadir,N., Saraiva,J.P., Zhang,J., Stolte,S., Gillor,O.,
            Harms,H. and Rocha,U.
  TITLE     Genome-centric analyses of 165 metagenomes show that mobile genetic
            elements are crucial for the transmission of antimicrobial
            resistance genes to pathogens in activated sludge and wastewater
  JOURNAL   Microbiol Spectr 12 (3), e0291823 (2024)
   PUBMED   38289113
REFERENCE   2  (bases 1 to 6947)
  AUTHORS   Abdulkadir,N.
  TITLE     Direct Submission
  JOURNAL   Submitted (24-MAR-2023) Environmental Microbiology, Helmholtz
            Center for Environmental Research -UFZ, Permoserstr 15, Leipzig
            04318, Germany
COMMENT     The annotation was added by the NCBI Prokaryotic Genome Annotation
            Pipeline (PGAP). Information about PGAP can be found here:
            https://www.ncbi.nlm.nih.gov/genome/annotation_prok/
            
            ##Genome-Assembly-Data-START##
            Assembly Method        :: SPAdes v. 3.11.1
            Genome Representation  :: Full
            Expected Final Version :: Yes
            Genome Coverage        :: Not Applicable
            Sequencing Technology  :: Illumina MiSeq
            ##Genome-Assembly-Data-END##
            
            ##Genome-Annotation-Data-START##
            Annotation Provider               :: NCBI
            Annotation Date                   :: 05/04/2023 04:57:10
            Annotation Pipeline               :: NCBI Prokaryotic Genome
                                                 Annotation Pipeline (PGAP)
            Annotation Method                 :: Best-placed reference protein
                                                 set; GeneMarkS-2+
            Annotation Software revision      :: 6.5
            Features Annotated                :: Gene; CDS; rRNA; tRNA; ncRNA
            Genes (total)                     :: 2,776
            CDSs (total)                      :: 2,750
            Genes (coding)                    :: 2,714
            CDSs (with protein)               :: 2,714
            Genes (RNA)                       :: 26
            tRNAs                             :: 24
            ncRNAs                            :: 2
            Pseudo Genes (total)              :: 36
            CDSs (without protein)            :: 36
            Pseudo Genes (ambiguous residues) :: 0 of 36
            Pseudo Genes (frameshifted)       :: 2 of 36
            Pseudo Genes (incomplete)         :: 32 of 36
            Pseudo Genes (internal stop)      :: 2 of 36
            ##Genome-Annotation-Data-END##
FEATURES             Location/Qualifiers
     source          1..6947
                     /organism="Micropruina sp."
                     /mol_type="genomic DNA"
                     /submitter_seqid="NODE_5304_length_6947_cov_4.097504"
                     /isolate="AMR_MDS_2195"
                     /isolation_source="Activated sludge of municipal
                     wastewater treatment plants"
                     /db_xref="taxon:2737536"
                     /environmental_sample
                     /geo_loc_name="Canada"
                     /lat_lon="49.25 N 123.1 W"
                     /collection_date="2015-06-16"
                     /metagenome_source="wastewater metagenome"
                     /note="metagenomic"
     gene            <1..897
                     /locus_tag="PLF56_04690"
     CDS             <1..897
                     /locus_tag="PLF56_04690"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_015071489.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="iron ABC transporter permease"
                     /protein_id="HOQ52892.1"
                     /translation="ALSVAARARTRAEQALRLRGDDTAEQPWRARRDLLPTVITAAVV
                     VVLLGVPMISLVVRSFVTADGWGLRNYQLLATTGEGSLSVPVWTAAVNSLVIAGQACA
                     LAVLLGVLVSLVASRRPRRRVLSGGVKLLDAAFMLPLGVSAVTVGFGFLITLNRPPFD
                     LRTSPILIPIAQAIVALPLVVRTLLPVLRAIDPHQREAAAMLGSGPGRVLATIDGALA
                     LRGLGLAVGFAFATSLGEFGATSFLSRPDHPTLPVVIFRLIGRPGADNFGMALAASVI
                     LAVVTAAVMAAAELVRPREAEL"
     gene            894..1871
                     /locus_tag="PLF56_04695"
     CDS             894..1871
                     /locus_tag="PLF56_04695"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_011753928.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_function: GO:0005524 - ATP binding [Evidence IEA];
                     GO_function: GO:0016887 - ATP hydrolysis activity
                     [Evidence IEA];
                     GO_function: GO:0042626 - ATPase-coupled transmembrane
                     transporter activity [Evidence IEA];
                     GO_function: GO:0140359 - ABC-type transporter activity
                     [Evidence IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="ABC transporter ATP-binding protein"
                     /protein_id="HOQ52893.1"
                     /translation="MSGLTVTGAEVRYGDLVAVDDVDLAVAPGEIVALLGASGSGKSS
                     LLRGVAGLEPLTAGRVCWSGDDLSSVPVHKRGFGMMFQDGQLFAHLSVEGNIAYGLFR
                     EPKQLRRERVTELLDVVGLAGYGKRRVSELSGGQAQRVALARSLAPRPRLLLLDEPLS
                     ALDRNLREHMVGVLADALHTTGTTALYVTHDQDEAFALADRVGVMENGRLLQTDAPAV
                     LWRHPDSQAVAAFLGYGPFVTGEVSQRLGGPDAPADRLVGIGPAALLPDSGGVVLPLI
                     EQRYQRGEVLVGVQLPDDQPATVRLPEKVPDATLQVSLDAAGCVSVPVG"
     gene            complement(1954..3300)
                     /locus_tag="PLF56_04700"
     CDS             complement(1954..3300)
                     /locus_tag="PLF56_04700"
                     /inference="COORDINATES: ab initio
                     prediction:GeneMarkS-2+"
                     /note="Derived by automated computational analysis using
                     gene prediction method: GeneMarkS-2+."
                     /codon_start=1
                     /transl_table=11
                     /product="hypothetical protein"
                     /protein_id="HOQ52894.1"
                     /translation="MSHPPHDRDVSQAQQPTARPPHLDDAIPAELQTTASITRAHPRR
                     QPRWRGALATAMIVLAGLVTPLAMLGTWAKVTLTDTDAFVATYAPVIRDPAMQAYLTD
                     QVSGAIVQQLKVEQLVGDAVAALQSTVGDRPRAAAAIGLLQQPAIDGIRDAVTRASAR
                     VIAADATAGAWREALKLSHSQAIGALSGDPATLTAITDQGLGLRIGPIVERVKTTLID
                     QGWTLAARIPAVDRTIIVVPSSDLQQVQQYYRLVVGLGAWLPWLVVGLMVGGVLIANN
                     RVRAIAGSGLAVALGAGAVVLGLGLTRRLLPTQVPASAMPSEVLQLLVATVTTDLSDV
                     ADALFTLGVVVALLGWWSGGGRTAQRLRAAWTTLATRIRRGRDALGLTTGRFGRWLFA
                     ARVWLRMLVVAAAIAFLAANRPLSPGLILATAGVSVALLLVAALLWPAESPTAAQS"
     gene            complement(3541..4782)
                     /locus_tag="PLF56_04705"
     CDS             complement(3541..4782)
                     /locus_tag="PLF56_04705"
                     /inference="COORDINATES: protein motif:HMM:NF014691.2"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="Fic family protein"
                     /protein_id="HOQ52895.1"
                     /translation="MEHADHSSSPRATNGSTTFGPITWETLPWRPTFDVEALSRRARN
                     SLPDTYDAAVPAQIAGCSFHLSTGLAADVEDATAAVMNLDHYVTTSLGQDHDIAPMQA
                     VLLRSESVASSQIENLTVGAKQLAIAEIGGAARGNAELVSRNVKALRAAIRLAGTLDA
                     TSILAMHEALLGGQDPRAGQWREEQVWIGSSSLSPAEAAFVPPQPLRVAAYMHDLARF
                     LARDDLPVLLHAALAHAQFETVHPFTDGNGRTGRALIQAMLRRGELTRRVTIPISAGL
                     LTDTASYFDALMSYRDGDIVPIVQQLCQASRTAALHGRWLIDELARTRALWLETVRPR
                     AGSASQTLLSVLVGQPAVTVVWVEERLGVSNTAARRAVDQLTAAGVIFETTDRRRNRV
                     FVARDVIDILDEFAARTGRRG"
     gene            4892..6373
                     /locus_tag="PLF56_04710"
     CDS             4892..6373
                     /locus_tag="PLF56_04710"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_008357968.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="peptide MFS transporter"
                     /protein_id="HOQ52896.1"
                     /translation="MSNTHPPMRDLPASGSADTRFFGQPRALVHIFGVEMWERFSFYG
                     MQGILLIYLYYSVTLGGLGIPEAVAGGIVGAYGGSVYLSTILGAWIADRILGAERVLF
                     VSAVVIMAGHIALAVLPGFVGVAVGLVLVAVGSGGLKATATSVVGTLYSAEDPRRDGG
                     FSLFYLGINLGAFFGPLLTGLLQSTLGFHWGFGLAALGMAAGLVQYSIGRKGLPATAG
                     EIPNPLPHNRYPLIGALAGGVLALIVVLVLIGVIRADNLALVVIYTVIAASIAYFAVI
                     LSSSRITADERSRVWGFVPLFLTSVAFWSLYQQQFTVLTVYSDTRLNRNLFGWEMPVS
                     WVQSINPVFIIVLSGVFAWVWTRLGDRQPSTPVKFALAAIVMGCAFLLFLPFAGGAEN
                     STPLLAVIGILFVFTVAELLLSPVGLSVTTKLAPESFHTQMVALFFLSVSLGTAIAGE
                     LAKLYDPSNETPYFSVLGLIAIGVGVVLLVCAKPVLSLMRGVR"
     gene            complement(6434..>6947)
                     /locus_tag="PLF56_04715"
     CDS             complement(6434..>6947)
                     /locus_tag="PLF56_04715"
                     /inference="COORDINATES: ab initio
                     prediction:GeneMarkS-2+"
                     /note="Derived by automated computational analysis using
                     gene prediction method: GeneMarkS-2+."
                     /codon_start=2
                     /transl_table=11
                     /product="hypothetical protein"
                     /protein_id="HOQ52897.1"
                     /translation="LMSETPWSDEVTERDAGAGYHFVELLALDDGSEADLDHDWGSQD
                     GPVRIQPGRVYLESFCDLQYPVIRLRAYRARPSDGQPDGLLGVWPVVFCGREVSVWSG
                     DSLPGEARPLRLEPSPGGQYGMRVAVTRIESDGDIDDYALARYEQDGDQPHDLESFSV
                     DFWPKVGADS"
BASE COUNT          998 a         2344 c         2441 g         1164 t
ORIGIN      
        1 gccttgtcgg tggccgctcg agctcgcacg cgggccgagc aggccttgag gctgcggggg
       61 gacgacaccg ccgagcaacc ctggcgcgca cgccgcgacc tgctgccgac ggtgatcacc
      121 gccgcggtcg tggtggtgct gctgggtgtg ccgatgatca gcctggtggt gcgctcgttc
      181 gtcaccgcgg acggctgggg cctgcgcaac tatcaactgc tcgctaccac cggcgagggc
      241 agcctgagcg tgccggtctg gacggccgcg gtcaactccc tcgtgatcgc cggccaggcc
      301 tgcgcgctgg ccgtgctgct cggcgtcctg gtcagcctgg tggcgtcccg gcgtccacgc
      361 cgccgcgttc tgtcgggcgg cgtcaagctg ctggacgccg ccttcatgct gcccctgggc
      421 gtctcggcgg tcacggtcgg gttcggcttc ttgatcacgt tgaaccgtcc gccgttcgac
      481 ctgcgcacct caccgatact cattccgatc gcgcaggcca tcgtggcgtt gccgctggtg
      541 gtgcgcacct tgctgccggt gctgcgagcg atcgacccgc accagcgcga ggcggcggcc
      601 atgctcggtt caggtcccgg acgggtgctg gccacgatcg acggggcgct cgccctgcgc
      661 gggctggggc ttgcggtggg gttcgcgttc gccacctcgc tgggcgagtt cggcgccacc
      721 tcgtttctct cccgtcccga ccacccgacg ctgcccgtgg tgatcttcag gctgatcgga
      781 cgtcccggcg ccgacaactt cggcatggcc ctggccgcct cggtgatcct ggccgtggtg
      841 accgccgcgg tgatggcggc agcggagctc gtccggccga gggaggcgga actgtgagcg
      901 gtctcaccgt caccggcgcc gaggttcgct acggcgacct ggtggccgtc gacgacgtcg
      961 acctggcggt cgcaccgggt gaaatcgtgg ccctgctggg tgcgtccgga tcgggcaaat
     1021 catcgctgct gcgcggcgtg gccggcctcg aaccgctgac cgccgggcgg gtctgctgga
     1081 gcggcgacga cctgtcgtcg gtgccggtgc acaaacgcgg tttcggcatg atgtttcagg
     1141 acggccaact gttcgcgcat ctgagcgtcg agggcaacat cgcctacggt ctgttccgcg
     1201 agccgaagca gctgcggcgc gagcgcgtca cggaactgct cgacgtggtg gggctcgccg
     1261 gctacggcaa acgccgggtc agcgagctct cgggcgggca ggcccaacgg gtggcgctgg
     1321 cccgctcgct ggcgccgcgt ccccggctgc tgctgctgga cgagccgctc tcggccctgg
     1381 atcgcaacct gcgcgagcac atggtcggtg tgctggccga cgcattgcac accaccggca
     1441 ccacggctct ctacgtgacc cacgatcagg acgaggcgtt cgccctggcc gatcgggtgg
     1501 gggtgatgga gaacggacgg ctgctgcaga ccgacgcccc ggcggtgctc tggcgccatc
     1561 cggactccca agcggtggcg gcgttcctcg gctacgggcc gttcgtgacg ggcgaggtgt
     1621 cgcagcggct cggcggcccc gatgcgccgg cggatcgact ggtcggcatc ggccctgccg
     1681 ccctgctgcc cgactctggc ggtgtcgtcc tgccgttgat cgagcagcgc taccagcgcg
     1741 gcgaggtgct ggtcggcgtg caactgccgg acgaccagcc cgcgaccgtc cggctgccgg
     1801 agaaggtgcc ggacgcgacc ctgcaggtca gcctggacgc cgccggctgc gtgagcgtgc
     1861 cggtcgggtg atctttcgat aggcgtcgcg atggtcgccc accgcgtcga tcgccgccag
     1921 cttccgcgtg atcggtcaag cggcgggtac gggttaggac tgcgccgcgg tgggtgattc
     1981 cgcgggccac agcagcgcgg ccaccaacag cagggcgacc gatacccccg cggttgcgag
     2041 gatcagcccg ggcgacagcg gacggttcgc cgccagaaag gcgatggcag cggccaccac
     2101 cagcatccgc agccacaccc tggcggcgaa aagccaacgg ccgaaccggc cggtggtcag
     2161 gccgagggcg tcgcgaccgc gccggatgcg ggtggcgagg gtcgtccagg cggcgcgaag
     2221 acgctgtgcc gtccgtccgc caccggacca ccagcccagc aaggccacca cgaccccgag
     2281 cgtgaacagg gcgtcggcga cgtccgaaag atcggtggtc accgtggcga ccagcagctg
     2341 cagcacctca ctaggcatgg cggacgccgg cacctgggta ggcagcaacc tgcgagtcag
     2401 gcccagcccc agcacgacgg ccccggcccc gagcgccacc gcgagtccgg aacccgcgat
     2461 cgcccggacg cggttgttcg cgatcagcac cccgccgacc atcagcccca cgaccagcca
     2521 gggcagccac gcgccgagcc cgacaaccag ccggtagtac tgctgcacct gttgcaggtc
     2581 gctcgagggc acgacgatta tcgtgcggtc gacggcgggg atgcgcgcgg cgagggtcca
     2641 accctggtcg atgagggtcg tcttgacccg ttcgacgatc ggtccgatcc gtaggccgag
     2701 cccctggtcg gtgatcgccg tgagggtggc gggatcgccg ctcaacgcgc cgatggcctg
     2761 gctgtgcgag agtttcaggg cctcacgcca cgcgccggcg gtggcgtcgg cggcgatcac
     2821 ccgggcggag gcccgcgtga ccgcgtcgcg aatgccgtcg atggccggct gctgcagcag
     2881 cccgatcgcc gcggccgccc ggggccggtc gccgaccgtc gactgcaggg cggccacggc
     2941 gtcaccgacc agttgttcga ccttgagctg ctggacgatg gcgcccgaca cctgatcggt
     3001 caggtaggcc tgcatcgccg ggtcgcggat caccggcgca taggtggcga cgaacgcgtc
     3061 ggtgtcggtc agggtgacct tggcccaggt gccgagcatc gccagcggcg tgaccagtcc
     3121 ggccagcacg atcatggccg tcgcgagggc accacgccaa cggggctgcc gacggggatg
     3181 ggcgcgggtg atggaggcgg tggtctgcag ttcggcaggg atcgcatcgt ccaggtgggg
     3241 cggacgtgcg gtcggctgct gagcctggga gacgtcccgg tcatggggtg gatggctcat
     3301 ggcatgcctg atctgctcgg tgcgacgcaa cgctcaaagt ctcccggatg aacgtccgat
     3361 agcgcagcac ccgggccggt accggctgcg actggtcgag cttgtcggga tccgccgggg
     3421 ggcgtggctc gatgtgcgca aggggattgc ccacccggaa gcgaacccgc acgcctgcca
     3481 gcagaagctc gaaggggatg cgagccctcc ataccggctt cggatcgcca cgctgcagcc
     3541 tcagccgcgg cggcccgttc gtgcggcgaa ctcgtcgaga atgtcgatga cgtcgcgggc
     3601 gacgaacacc cggttgcgcc gccggtcggt ggtctcgaag atgacgccgg ccgcagtgag
     3661 ttgatcgacg gcgcggcggg cagcggtgtt cgacacaccc agtcgttctt cgacccagac
     3721 cacggtgacg gcgggctgac ccaccaacac gctgagcagg gtttgactgg ccgagccggc
     3781 ccgtggccgc acagtctcga gccacaacgc tcgggtgcgg gcgagctcgt cgatcagcca
     3841 gcgtccgtgc agtgctgcag tccgggaggc ctggcacagc tgttgcacga tggggacgat
     3901 gtcgccgtcg cggtagctca tcagcgcgtc gaagtaggac gccgtgtcgg tgagcaggcc
     3961 cgccgagatc ggtatggtca cacgcctggt cagttcgccg cggcgcagca tcgcctgtat
     4021 cagggcacgg ccggtgcgtc cgttgccgtc ggtgaacggg tggaccgtct cgaactgcgc
     4081 atgagcgagc gcggcgtgga gcagtaccgg taggtcatca cgggccagga acctcgccag
     4141 gtcgtgcatg taggcagcga cccgtaaggg ctgcggagga acgaaggcag cttcagcggg
     4201 gctcaggctg gacgagccga tccacacctg ttcctcgcgc cactgacccg cgcgtggatc
     4261 ctgaccccca agcagggcct cgtgcatagc caggatggac gtcgcgtcga gtgtgccggc
     4321 cagccggatg gctgctcgga gagctttcac gttcctggag acaagctcgg cgttgccgcg
     4381 ggctgcgccc ccgatctcgg cgatggccag ctgcttcgct cccacggtga ggttctcgat
     4441 ctgggaggag gcgaccgact ccgatcgcag aagcacagcc tgcatgggcg cgatgtcgtg
     4501 atcctgacca aggctggtcg tcacatagtg atcgaggttc atcacagcgg ccgtcgcatc
     4561 ctccacgtca gccgccaggc cggtggacag gtggaacgaa caccctgcga tctgggccgg
     4621 aaccgccgcg tcgtaggtgt caggcaggct gttgcgagcc cggcgagaca gtgcctccac
     4681 gtcgaaggtc ggccgccagg ggagggtctc ccacgtgatc ggcccaaacg tcgtgctgcc
     4741 gttcgtggcg cgtggcgagg aactgtgatc agcatgttcc atgcaggcac ggtatcgcac
     4801 gatgtagcat taaagtgcgg tacgagcaat gctaaggaca catcgtccgg ggcctgaccg
     4861 aatcgctgct ggaaggtcta gcgtgccttc catgagcaac actcaccccc cgatgcgcga
     4921 tttacccgcg tcgggctcag cggacacccg tttcttcggg cagccgcgcg ccctcgtcca
     4981 catcttcggc gtggagatgt gggagcgctt cagcttctac ggcatgcagg gcatcctgct
     5041 catctacctg tactactcgg tcacactggg cggcctcggc atccccgagg cggtcgcggg
     5101 cggcatcgtc ggtgcctacg ggggctcggt ctacctgtcg acgatcctcg gtgcctggat
     5161 agccgatcgc atcctgggcg ccgagcgggt gctgttcgtc agtgccgtgg tgatcatggc
     5221 aggtcacatc gccctggcgg tactgcccgg cttcgtgggg gtcgcggtgg gcctggtgct
     5281 ggtcgccgtt gggtccggcg gcctgaaggc aaccgcgacc agcgtcgtcg gcactctgta
     5341 ctcagccgag gacccccgcc gcgatggcgg gttctcgctg ttctacctgg gaatcaacct
     5401 gggcgcgttc ttcgggccgc tgctcaccgg actgttgcag agcacacttg gattccactg
     5461 gggcttcggg ctggccgccc tcggcatggc cgcaggcctg gtgcagtact cgatcggccg
     5521 caagggcctg ccggcaacgg cgggcgagat cccgaacccg ctcccacaca accggtaccc
     5581 gttgatcggt gcgcttgccg gcggcgtcct ggccctgatc gtggtgttgg tgctgattgg
     5641 cgtgatccgc gcggacaacc tggccctggt ggtgatctac acggtcatcg cggcgtcgat
     5701 cgcctacttc gccgtgatcc tctccagcag ccggatcacc gccgacgagc gctcccgggt
     5761 gtgggggttc gtcccgttgt tcctcacctc ggtggccttc tggtcgctgt accagcagca
     5821 gttcaccgtg ttgacggtgt actccgacac ccgcctgaac cgcaaccttt tcggctggga
     5881 gatgccggtg tcgtgggtgc agtcgatcaa tccggtattc atcattgtgc tgtccggcgt
     5941 gttcgcctgg gtgtggacgc ggctcggcga tcgccagccc tcgaccccgg tcaagttcgc
     6001 cttggcggcc atcgtcatgg gctgcgcctt cctgctgttc ctgccgttcg ccggcggcgc
     6061 ggagaactcc accccgttgc tcgccgtcat cggcatcctg ttcgtcttca ccgtcgcgga
     6121 actgctgctg tcgccggtcg ggttgtcggt gaccaccaag cttgcacccg agtcgttcca
     6181 cacccagatg gtcgccctgt tcttcctctc cgtgtccctg ggcacagcca tcgccggcga
     6241 gctggccaag ctgtacgacc cgtccaacga aacgccctac ttctcggtgc tcgggctgat
     6301 cgcgatcggc gtcggcgtcg tcctgctggt gtgcgccaag cccgtgctga gcctgatgcg
     6361 aggggtgcgc tgaccgacgc tgacaaccgt ccgggtgagt cacgcgcacg ggtgattgac
     6421 atgccgactg atctcagcta tccgctccca ccttcggcca gaagtcgacg gagaagctct
     6481 cgagatcgtg tggttggtcc ccgtcttgtt cgtagcgggc gagcgcgtag tcgtcgatgt
     6541 cgccgtcgga ctcgatacgc gtcaccgcta cccgcatgcc gtactgaccg ccagggctcg
     6601 gctcaagccg gagtggacga gcctcgccgg gcagcgagtc gccactccac acgctgactt
     6661 cacggccgca gaagacgacg ggccacacac ccagcaggcc gtccggctga ccatccgacg
     6721 gccgcgcgcg atatgcgcgt agccggatca cggggtattg gaggtcgcaa aaggactcga
     6781 gatacacccg gcctggttgg atgcggactg gcccgtcttg gctgccccag tcgtggtcca
     6841 agtccgcctc ggaaccatcg tccagtgcca gcagctcaac gaagtggtat ccagccccag
     6901 cgtcgcgctc ggtgacctcg tcagaccacg gggtctcgct catgagg
//
LOCUS       DAOSUG010000099         6909 bp    DNA     linear   ENV 10-JUN-2024
DEFINITION  MAG TPA_asm: Micropruina sp. isolate AMR_MDS_2195
            NODE_5333_length_6909_cov_4.883426, whole genome shotgun sequence.
ACCESSION   DAOSUG010000099 DAOSUG010000000
VERSION     DAOSUG010000099.1
DBLINK      BioProject: PRJNA925477
            BioSample: SAMN32864262
            Sequence Read Archive: SRR4236673
KEYWORDS    WGS; ENV; Metagenome Assembled Genome; MAG; Third Party Data; TPA;
            TPA:assembly.
SOURCE      Micropruina sp. (wastewater metagenome)
  ORGANISM  Micropruina sp.
            Bacteria; Bacillati; Actinomycetota; Actinomycetes;
            Propionibacteriales; Nocardioidaceae; Micropruina.
REFERENCE   1  (bases 1 to 6909)
  AUTHORS   Abdulkadir,N., Saraiva,J.P., Zhang,J., Stolte,S., Gillor,O.,
            Harms,H. and Rocha,U.
  TITLE     Genome-centric analyses of 165 metagenomes show that mobile genetic
            elements are crucial for the transmission of antimicrobial
            resistance genes to pathogens in activated sludge and wastewater
  JOURNAL   Microbiol Spectr 12 (3), e0291823 (2024)
   PUBMED   38289113
REFERENCE   2  (bases 1 to 6909)
  AUTHORS   Abdulkadir,N.
  TITLE     Direct Submission
  JOURNAL   Submitted (24-MAR-2023) Environmental Microbiology, Helmholtz
            Center for Environmental Research -UFZ, Permoserstr 15, Leipzig
            04318, Germany
COMMENT     The annotation was added by the NCBI Prokaryotic Genome Annotation
            Pipeline (PGAP). Information about PGAP can be found here:
            https://www.ncbi.nlm.nih.gov/genome/annotation_prok/
            
            ##Genome-Assembly-Data-START##
            Assembly Method        :: SPAdes v. 3.11.1
            Genome Representation  :: Full
            Expected Final Version :: Yes
            Genome Coverage        :: Not Applicable
            Sequencing Technology  :: Illumina MiSeq
            ##Genome-Assembly-Data-END##
            
            ##Genome-Annotation-Data-START##
            Annotation Provider               :: NCBI
            Annotation Date                   :: 05/04/2023 04:57:10
            Annotation Pipeline               :: NCBI Prokaryotic Genome
                                                 Annotation Pipeline (PGAP)
            Annotation Method                 :: Best-placed reference protein
                                                 set; GeneMarkS-2+
            Annotation Software revision      :: 6.5
            Features Annotated                :: Gene; CDS; rRNA; tRNA; ncRNA
            Genes (total)                     :: 2,776
            CDSs (total)                      :: 2,750
            Genes (coding)                    :: 2,714
            CDSs (with protein)               :: 2,714
            Genes (RNA)                       :: 26
            tRNAs                             :: 24
            ncRNAs                            :: 2
            Pseudo Genes (total)              :: 36
            CDSs (without protein)            :: 36
            Pseudo Genes (ambiguous residues) :: 0 of 36
            Pseudo Genes (frameshifted)       :: 2 of 36
            Pseudo Genes (incomplete)         :: 32 of 36
            Pseudo Genes (internal stop)      :: 2 of 36
            ##Genome-Annotation-Data-END##
FEATURES             Location/Qualifiers
     source          1..6909
                     /organism="Micropruina sp."
                     /mol_type="genomic DNA"
                     /submitter_seqid="NODE_5333_length_6909_cov_4.883426"
                     /isolate="AMR_MDS_2195"
                     /isolation_source="Activated sludge of municipal
                     wastewater treatment plants"
                     /db_xref="taxon:2737536"
                     /environmental_sample
                     /geo_loc_name="Canada"
                     /lat_lon="49.25 N 123.1 W"
                     /collection_date="2015-06-16"
                     /metagenome_source="wastewater metagenome"
                     /note="metagenomic"
     gene            complement(177..779)
                     /locus_tag="PLF56_04720"
     CDS             complement(177..779)
                     /locus_tag="PLF56_04720"
                     /inference="COORDINATES: protein motif:HMM:NF013828.2"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_function: GO:0005524 - ATP binding [Evidence IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="ATP-binding protein"
                     /protein_id="HOQ52898.1"
                     /translation="MMTGVTHSKVDGLIRRAGLRYPTADLRRIDLVEERGLDRGVIAQ
                     LATCSFIERSHNVVFQGFTGSGKSYLGCALAKQACLHRIRAHYIRMPDLAEAWHLARD
                     KPQGQMKFLRKYAAFTLLVVDEWLLDPPDESMRSMLLELLERRYDTTSTLFCTQYAKK
                     DWHQRLGGGVHADAIMDRIVHNTIWIDTGNHNMREHTATK"
     gene            763..1293
                     /locus_tag="PLF56_04725"
     CDS             763..1293
                     /locus_tag="PLF56_04725"
                     /inference="COORDINATES: protein motif:HMM:NF013695.2"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_component: GO:0016020 - membrane [Evidence IEA];
                     GO_function: GO:0008324 - monoatomic cation transmembrane
                     transporter activity [Evidence IEA];
                     GO_process: GO:0006812 - monoatomic cation transport
                     [Evidence IEA];
                     GO_process: GO:0055085 - transmembrane transport [Evidence
                     IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="cation transporter"
                     /protein_id="HOQ52899.1"
                     /translation="MTPVIMGRIKLRYAEQLSDKVLRADADMNKADWLTGLATAVGVL
                     GIGLGFWWADAVAALVVSISISKDGWDNTANAIRDLMDTRPTTFDNSEVDPVIAKVNA
                     AVMDDPRVVGVQTRARDEGHVLHLEVFVQVDRLVVEVAWLEELERRCNEVDWRAGDVV
                     VTVTTEPHPVADARLE"
     gene            1466..4576
                     /locus_tag="PLF56_04730"
     CDS             1466..4576
                     /locus_tag="PLF56_04730"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_013160163.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_function: GO:0004553 - hydrolase activity, hydrolyzing
                     O-glycosyl compounds [Evidence IEA];
                     GO_process: GO:0005975 - carbohydrate metabolic process
                     [Evidence IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="glycoside hydrolase family 2 TIM barrel-domain
                     containing protein"
                     /protein_id="HOQ52900.1"
                     /translation="MITPRHHENLQVVNVSVLPRRAYYVPASRPMGPLVGDREQSDRL
                     QMLNGPWWFQYFESTYDVPEQFYLGDDLADAYGEVPVPSVWQNLGFDSHQYTNIRYPI
                     PLDPPHVPQENPCGAYIRDFDYAPDPDAPRVHLNFEGVDSCYYVWLNGSYVGYSQISH
                     ATSEFDVTDLLVAGTNRLAVLVLKWGVGTYLEDQDKFRTSGIFRDVYLLQRPESAVFD
                     YFTTTQLHPDRAVVHVRASFSGDPVPTRITLTDADGEPVAEADLRHAPADDEFTHGTE
                     LGIPTPHLWNAEDPYLYTLTIATEREAIVDRVGVREIQTNGNVVQVNGRHIRFRGVNR
                     HDSDPVTGPVISLDQLMTDLRLMKQHNVNAIRSAHYPNSPYFYQLCDELGFYVMNEAD
                     NESHGTQAQYLADPSFPNQVLHWNERIADNPDFLPATMDRMQACVRREKNRPSVVMWS
                     AGNECAYGITLEESLRWTKDYDPTRLTVYESSFYDDKKRKYDYSVIDIYSRMYPALTE
                     VQDYLDSQPDKPFLLLEYCHAMGNGPGDFQDYLEVVESDPVMCGGFVWEWCDHAVHTG
                     TTEDGRAIYYYGGDHGEIIHDGNFCVDGLVSPDRQPHVGLLEFANVHRPVRVAGFDQT
                     TGELRLHNHLDFTDLAGTVSLSYEVTRDGELIEEGTIETLPTIPPRAVGSVNCNIPVP
                     ESGRCYLKVTYHSLVATELVPAGHVLGFDEVPLEVADPRNQTALELLPPDDQDPPALD
                     VQADERFITISGASFEYVLDRTDGLFKTMNVGGEPLLTRPMEVNIWRAPTDNDMYIKA
                     KWYLAQYPHARARAYDTEYSVGPDGVTITSSMGLLAPAVQRIAALETVWHVSPAGAVH
                     VDMTVRKDPEFPMFPRFGLRLFLDGAFDQVTYYGRGPHESYPDKHRASSHGRYSSSVP
                     ELHVDYLRPQENGSHHDCDYVIAHDDAARAIAAVGDQTFSFNVSPYTQEQLADARRNV
                     DLTGSGETVWCLDYAHNGIGSNSCGPELLEKYRLDAPEFRFALTLVPSASVESTIPHH
                     RNEANA"
     gene            4573..5994
                     /locus_tag="PLF56_04735"
     CDS             4573..5994
                     /locus_tag="PLF56_04735"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_014847726.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_component: GO:0016020 - membrane [Evidence IEA];
                     GO_function: GO:0015486 -
                     glycoside-pentoside-hexuronide:cation symporter activity
                     [Evidence IEA];
                     GO_process: GO:0006812 - monoatomic cation transport
                     [Evidence IEA];
                     GO_process: GO:0008643 - carbohydrate transport [Evidence
                     IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="glycoside-pentoside-hexuronide (GPH):cation
                     symporter"
                     /protein_id="HOQ52901.1"
                     /translation="MSENTATVVDDPREKKYLKWYNKVGYGSGDVGGNVVYAFIAFFV
                     MIYLTDTMGLNPGIIGTLIMASRIFDGISDIFFGSLIDKTNTRMGKARPWMFWAYFGC
                     AAMIVAIFAVPPSLGDFAMYTWFFITYVLLNAVFFTANNIAYSTLTALITKNAEERVQ
                     MGSIRFVFAFGTSLLIQVSTVGLVQFFGGGAEAWRSVAIIFALVGLTVNTISVFSVRE
                     LPPEVLYARPDEDAEEEQETIAEDKPTLLASLKLLIANKYYLMIVLVFTLGQVVTAML
                     GMGIYFMTYILGDVNYFSTFAWAINVPMILTLLATPFVVKWARGIYWVNLYGYIIAFI
                     GRALVIVAAFMGSIPLMLVFTAVGSIGMAPLQGTLNALIAEASEHTFMTKKKRIDGMM
                     FSATSLGVKIGGGIGVAVTGWLLAASGYVGGAEVQPESAVTMIQFMYLWMPAIILAIV
                     AVVLSRLKVESANKKLRRELAES"
     gene            6071..>6909
                     /locus_tag="PLF56_04740"
     CDS             6071..>6909
                     /locus_tag="PLF56_04740"
                     /inference="COORDINATES: ab initio
                     prediction:GeneMarkS-2+"
                     /note="Derived by automated computational analysis using
                     gene prediction method: GeneMarkS-2+."
                     /codon_start=1
                     /transl_table=11
                     /product="hypothetical protein"
                     /protein_id="HOQ52902.1"
                     /translation="MKGPFPRVPGMPSSPRSSITRISSTLAIGFVAAALAACSGTGDA
                     LGEPAEANDDVVVQQEQPQQDDGANPGDSDVDDDGVAGAADDADGGNAIILPARTIDA
                     TVSVGGLEYTIGEVRVLDLDDEAGRLPQRTHGLQVDIDVEAFNPTDSQSMVNLMATLS
                     WVDPESGLLHQGFGSPPQGAGVPGGGRANLALEYQVDGMEVDTFSLESAALRFGNEGQ
                     HAAVVPLGDEGELKSKFPHFQEQMVGVTVELEDVSWTIDEAFIGYNHGNSRLGDDQVL
                     LEIA"
BASE COUNT         1285 a         2215 c         2191 g         1218 t
ORIGIN      
        1 caagcgccta tgtcctcata gtcacccgaa atccgaccta gaggtccaac tcaggtcgaa
       61 ctgagtattc gaggctgacg gggccactgg atactgcctc tgggggccgg tgatattgca
      121 ggtgggggcc accagtggcg ctggcggggg ccagagcctc cgccagcgcc ggtcggttac
      181 ttggtggcgg tgtgttctct catgttgtgg ttgccggtgt cgatccagat ggtgttgtgg
      241 acgatgcggt ccatgatggc gtcggcgtgg actccgccgc cgaggcgttg gtgccagtcc
      301 ttcttcgcgt attgggtgca gaagagcgtg gaggtggtgt cgtagcggcg ctcgagtagc
      361 tccagcagca tgctgcgcat gctctcatcg ggcgggtcaa ggagccactc atcgaccaca
      421 aggagggtga aggccgcgta tttacggagg aacttcatct gcccctgcgg cttgtcccgg
      481 gccaggtgcc aagcttcggc gaggtcgggc atgcggatgt agtgggcccg gatccggtgg
      541 aggcaggcct gtttcgccag ggcgcagccg aggtaggact tcccggagcc ggtgaagcct
      601 tggaacacga cgttgtggga gcgttcgatg aacgaacacg tcgccagctg cgcgatcacg
      661 ccgcggtcga ggcctcgttc ctcgacgagg tcgattcggc gcagatctgc ggtgggatag
      721 cgaagccccg ccctgcggat gaggccgtcg accttggagt gggtgacgcc cgtcatcatg
      781 ggccggatca agctgcgcta tgccgagcag ttgagcgaca aggtcctgcg cgccgatgcg
      841 gacatgaaca aggccgactg gctgaccggt ctggccactg ctgttggcgt gctcggcatc
      901 gggttgggat tctggtgggc ggacgccgtc gccgctctcg tggtctccat cagcatctcg
      961 aaagacggct gggacaacac cgccaacgcc atccgcgacc tgatggacac ccgccccacc
     1021 accttcgaca actcggaggt ggaccccgtg atcgccaagg tgaacgcagc cgtcatggat
     1081 gacccgcggg tggtcggcgt ccagacccgc gctcgggacg agggccacgt gctgcacctg
     1141 gaggtcttcg tgcaggtgga caggctggtg gtggaagtgg cttggctgga ggaactcgaa
     1201 cggcgctgca acgaggtgga ctggcgggca ggcgacgtcg tggtcaccgt caccaccgaa
     1261 cctcatcccg tggccgatgc ccggctggag tgagcatgcc aagctccgtc tgacagggga
     1321 tcaaggaact ttctgaatag cgggcgccgc gccaggctcc atggtatcaa tgatgatcaa
     1381 gtactgcgtc agtcgctgga cccgactccc agtcgagggg gcggtcccag cggcgcacgt
     1441 ccggcaggaa gcaacggagc gccccatgat caccccccgt catcacgaga acctgcaggt
     1501 cgtgaacgta agcgtcctgc cgcgacgggc gtactacgtc ccggcatccc ggcccatggg
     1561 gccgctcgtt ggggatcggg agcaatcgga ccggctccag atgctcaacg gcccatggtg
     1621 gttccagtac ttcgagagca cctatgacgt gccggagcag ttctacctcg gtgatgacct
     1681 ggcagacgca tatggcgagg tgcccgtgcc cagcgtgtgg cagaacctgg gcttcgattc
     1741 ccaccagtac accaacatcc gctacccgat cccgctcgac cccccgcacg tgccccagga
     1801 gaatccctgc ggcgcctaca tccgcgattt cgactacgcg ccggacccgg acgcgccccg
     1861 ggtccacctc aacttcgagg gagtcgattc ctgctactac gtgtggctga acggcagcta
     1921 cgttggctac agccagatct cccacgccac cagcgagttc gacgtgaccg acctgctcgt
     1981 cgccgggacg aaccggctgg cggtcctcgt cctcaagtgg ggagtgggca cgtaccttga
     2041 ggaccaggac aagttccgca cgagcgggat cttccgcgat gtctacctgc tgcagcgccc
     2101 ggaatcggcg gttttcgact acttcaccac cacacaactc cacccggatc gtgccgtggt
     2161 ccacgtccgc gcatccttca gcggcgatcc cgtcccgacc cgcatcacgc tcaccgacgc
     2221 cgacggggaa cccgtcgccg aggccgactt gcgacacgcc cccgccgacg acgagttcac
     2281 ccacggcacc gagctcggga tcccgacccc gcacctctgg aacgccgagg atccgtacct
     2341 gtacacgctg accatcgcca ccgaacggga ggccatcgtg gaccgcgtgg gggtcaggga
     2401 aatccagacg aacggcaacg tggtgcaggt caatggtcgc cacatcaggt tccggggcgt
     2461 caaccggcac gattccgatc ccgtcacggg cccggtcatc agcctggacc aactcatgac
     2521 ggatctgagg ttgatgaaac agcacaacgt caacgcgatc cgcagcgccc actacccgaa
     2581 ctcgccctac ttctaccaac tctgcgacga gctcgggttc tacgtcatga acgaggccga
     2641 caacgagagc cacggcacgc aggcccaata cctggccgat ccgtcctttc ccaaccaggt
     2701 actgcactgg aacgagcgca tcgcggacaa cccggacttc ctgccggcca cgatggatcg
     2761 catgcaagcc tgcgtgcgac gtgagaagaa ccgtccctcc gtcgtgatgt ggtccgcggg
     2821 caacgagtgc gcctacggca tcaccctgga ggaatccctg cgctggacca aggactacga
     2881 ccccacccgc ctgaccgtct acgaaagctc cttctacgac gacaagaagc gcaagtacga
     2941 ctactcggtg atcgacatct acagccgcat gtaccccgct ttgaccgagg tgcaggacta
     3001 cctcgactcg cagcccgaca agcccttcct gctgctggag tactgccacg ccatgggcaa
     3061 cggtccgggt gatttccagg actatctcga ggtcgttgag tccgatccgg tcatgtgcgg
     3121 cggcttcgtg tgggagtggt gcgatcacgc ggtccacacg gggaccaccg aggacggtcg
     3181 tgcgatctac tactacggcg gtgaccacgg cgagatcatc cacgacggca atttctgcgt
     3241 ggacggcctg gtcagcccgg accggcaacc gcatgtgggc ctgctggaat tcgccaacgt
     3301 ccaccgcccg gtccgggtgg cgggcttcga ccagaccacc ggcgaactgc ggctgcacaa
     3361 ccacctcgac ttcaccgatc tcgccggcac cgtgagcctc agctacgagg tgacgcgcga
     3421 cggcgagctc atcgaggaag ggaccatcga gacattgccg accatcccgc cgcgcgccgt
     3481 cggctccgtc aactgcaaca tcccggtccc cgagtcggga cgctgctacc tcaaggtcac
     3541 ctatcactcc ctcgttgcca cggagctggt gccggccggt cacgtcctgg gcttcgacga
     3601 ggtgccgctg gaggttgcgg acccgcggaa ccagacggcg cttgagctcc tgccgcccga
     3661 cgaccaggac ccgccggcgc tcgacgtgca ggccgatgaa cgcttcatca cgatcagtgg
     3721 cgcttctttc gagtacgtcc tggaccgaac cgatggcctg ttcaagacga tgaacgtcgg
     3781 aggcgagccc ctgctgacgc gcccgatgga ggtgaacatc tggcgggccc cgacggacaa
     3841 cgacatgtac atcaaggcga agtggtacct ggcgcagtac ccccacgctc gcgcccgcgc
     3901 gtacgacacc gagtactcgg tgggtccgga tggagtgacg atcaccagct cgatgggact
     3961 tctcgccccg gccgtccagc ggatcgcggc gttggagacg gtgtggcacg tgagccctgc
     4021 cggtgcggtc cacgtcgaca tgacggtgcg caaggatccc gagttcccca tgttcccgcg
     4081 cttcggcctg cgcctgttcc tggacggagc cttcgatcag gtgacgtact acgggcgcgg
     4141 cccccacgag agttacccgg acaagcatcg ggcgagttcc cacgggcggt actcctcctc
     4201 ggtgcccgag ctgcatgtgg actacctgcg gccgcaggag aacggcagtc accacgattg
     4261 cgactacgtg atcgcccatg acgacgctgc gcgagccatt gccgcagtgg gggaccagac
     4321 gttcagcttc aacgtctcgc cgtacacgca ggaacaactg gccgatgcga gacgcaatgt
     4381 ggatctgacc ggttcaggcg agacggtgtg gtgcctggac tacgcccaca acggcatcgg
     4441 ttcgaacagt tgcggcccgg agcttctcga gaagtaccgg ctcgacgcgc cggagttccg
     4501 gtttgctctc acgctcgtcc cctcggcgag cgttgaatca acaatcccgc atcacaggaa
     4561 cgaggccaac gcatgagcga gaacaccgcg acagtggttg acgacccgcg agagaagaag
     4621 tacctgaagt ggtacaacaa ggtcgggtac ggctcggggg acgtgggcgg caacgtcgtc
     4681 tacgccttca tcgcgttctt cgtgatgatc tacctgaccg acacgatggg gttgaacccc
     4741 ggcatcatcg gcaccctgat catggcctcg agaatcttcg atggcatctc cgacatcttc
     4801 ttcggttcac tgatcgacaa gacgaacacc aggatgggca aggcccgtcc ctggatgttc
     4861 tgggcatact tcgggtgtgc cgccatgatc gtcgcgatct tcgcggtccc accgagtctc
     4921 ggcgacttcg cgatgtacac gtggttcttc atcacctacg tcctgctcaa tgctgtcttc
     4981 ttcacggcca acaacatcgc atactcgacg ctgacggcgc tgatcaccaa gaacgccgaa
     5041 gagcgcgtgc agatgggctc catccgtttc gtcttcgcct tcgggacgag cctcctcatc
     5101 caggtgtcca cggtgggcct cgtgcagttc ttcggcggtg gcgcagaggc gtggcgcagc
     5161 gtggccatca tcttcgcgct cgtgggcctg accgtgaaca cgatctcggt gttctccgtg
     5221 cgggaactgc cacccgaggt gctctacgct cgcccggacg aggacgctga ggaagagcag
     5281 gaaacgatcg ccgaggacaa gccgaccctg cttgcgtcgc tcaagctgct gatcgcgaac
     5341 aagtactacc tgatgatcgt gctggtgttc accctcggtc aggtcgtcac ggccatgctg
     5401 ggcatgggga tctacttcat gacctacatc ctcggggatg tgaactactt cagtacgttc
     5461 gcctgggcga tcaacgttcc gatgatcctc acattgctgg cgacgccatt cgtggtgaag
     5521 tgggccagag ggatctattg ggtcaatctg tacggataca tcatcgcgtt cattggacgc
     5581 gcactggtga tcgttgccgc cttcatgggc agcatcccgc tcatgctggt gttcacggcc
     5641 gtgggatcga tagggatggc tccgctgcag gggacgttga atgctctcat cgccgaagcg
     5701 tcagagcaca ccttcatgac caagaagaag cgcatcgacg gcatgatgtt ctccgccacg
     5761 tccctgggcg tgaagatcgg tggtggcatc ggtgtggccg tgactggatg gctgctcgcc
     5821 gcgagcggct acgtcggcgg cgcggaggtg cagccggaat cggcggtgac catgatccag
     5881 ttcatgtacc tgtggatgcc ggccatcatt cttgccatcg tggccgtggt tctgtcccgg
     5941 ttgaaggtgg agagcgcgaa caagaagctt cgccgagagc tggccgagag ctgagcgtcg
     6001 gacgacgatc cactccaccg agcccgacat ccactccgaa ccagacggcc gacggaggac
     6061 tgaaaggttt gtgaaaggcc ccttccctag agtccccggc atgccttctt caccgagatc
     6121 atcaatcaca agaatctcct ccactctggc gattggcttc gtcgccgcag ccctggccgc
     6181 gtgttcagga accggcgacg ccctggggga acctgccgag gccaacgacg acgttgttgt
     6241 ccagcaggaa cagccgcagc aggatgatgg cgccaacccc ggcgactccg acgtggatga
     6301 cgacggagtg gccggggcgg cggacgacgc cgatgggggg aacgccatca tcctgcctgc
     6361 gcgcaccatc gacgccaccg tgtccgtcgg tggcctggag tacaccattg gcgaggtcag
     6421 ggtgctggac ctggacgatg aggccggcag actgccccag cgaacccacg gtctgcaggt
     6481 ggacatcgac gtggaagcct tcaatcccac cgattcacag tccatggtca acctcatggc
     6541 gaccctcagc tgggtggatc ccgaaagcgg actcctccac caaggattcg gctccccacc
     6601 ccagggcgcg ggagtgccgg ggggaggtcg tgcgaatctt gcactggagt accaggttga
     6661 tgggatggag gtcgacacgt tctctctgga gagcgccgcc ctccgattcg gcaacgaggg
     6721 ccagcacgca gcggtggtcc ccctcggcga cgagggagag ctcaagtcga agttcccgca
     6781 cttccaggag cagatggtgg gcgtcaccgt cgaactggag gacgtgagct ggacgatcga
     6841 cgaggcgttc atcggctaca accacggcaa ttcacggctg ggcgatgacc aggtgctcct
     6901 ggagatcgc
//
LOCUS       DAOSUG010000100         6881 bp    DNA     linear   ENV 10-JUN-2024
DEFINITION  MAG TPA_asm: Micropruina sp. isolate AMR_MDS_2195
            NODE_5357_length_6881_cov_5.078230, whole genome shotgun sequence.
ACCESSION   DAOSUG010000100 DAOSUG010000000
VERSION     DAOSUG010000100.1
DBLINK      BioProject: PRJNA925477
            BioSample: SAMN32864262
            Sequence Read Archive: SRR4236673
KEYWORDS    WGS; ENV; Metagenome Assembled Genome; MAG; Third Party Data; TPA;
            TPA:assembly.
SOURCE      Micropruina sp. (wastewater metagenome)
  ORGANISM  Micropruina sp.
            Bacteria; Bacillati; Actinomycetota; Actinomycetes;
            Propionibacteriales; Nocardioidaceae; Micropruina.
REFERENCE   1  (bases 1 to 6881)
  AUTHORS   Abdulkadir,N., Saraiva,J.P., Zhang,J., Stolte,S., Gillor,O.,
            Harms,H. and Rocha,U.
  TITLE     Genome-centric analyses of 165 metagenomes show that mobile genetic
            elements are crucial for the transmission of antimicrobial
            resistance genes to pathogens in activated sludge and wastewater
  JOURNAL   Microbiol Spectr 12 (3), e0291823 (2024)
   PUBMED   38289113
REFERENCE   2  (bases 1 to 6881)
  AUTHORS   Abdulkadir,N.
  TITLE     Direct Submission
  JOURNAL   Submitted (24-MAR-2023) Environmental Microbiology, Helmholtz
            Center for Environmental Research -UFZ, Permoserstr 15, Leipzig
            04318, Germany
COMMENT     The annotation was added by the NCBI Prokaryotic Genome Annotation
            Pipeline (PGAP). Information about PGAP can be found here:
            https://www.ncbi.nlm.nih.gov/genome/annotation_prok/
            
            ##Genome-Assembly-Data-START##
            Assembly Method        :: SPAdes v. 3.11.1
            Genome Representation  :: Full
            Expected Final Version :: Yes
            Genome Coverage        :: Not Applicable
            Sequencing Technology  :: Illumina MiSeq
            ##Genome-Assembly-Data-END##
            
            ##Genome-Annotation-Data-START##
            Annotation Provider               :: NCBI
            Annotation Date                   :: 05/04/2023 04:57:10
            Annotation Pipeline               :: NCBI Prokaryotic Genome
                                                 Annotation Pipeline (PGAP)
            Annotation Method                 :: Best-placed reference protein
                                                 set; GeneMarkS-2+
            Annotation Software revision      :: 6.5
            Features Annotated                :: Gene; CDS; rRNA; tRNA; ncRNA
            Genes (total)                     :: 2,776
            CDSs (total)                      :: 2,750
            Genes (coding)                    :: 2,714
            CDSs (with protein)               :: 2,714
            Genes (RNA)                       :: 26
            tRNAs                             :: 24
            ncRNAs                            :: 2
            Pseudo Genes (total)              :: 36
            CDSs (without protein)            :: 36
            Pseudo Genes (ambiguous residues) :: 0 of 36
            Pseudo Genes (frameshifted)       :: 2 of 36
            Pseudo Genes (incomplete)         :: 32 of 36
            Pseudo Genes (internal stop)      :: 2 of 36
            ##Genome-Annotation-Data-END##
FEATURES             Location/Qualifiers
     source          1..6881
                     /organism="Micropruina sp."
                     /mol_type="genomic DNA"
                     /submitter_seqid="NODE_5357_length_6881_cov_5.078230"
                     /isolate="AMR_MDS_2195"
                     /isolation_source="Activated sludge of municipal
                     wastewater treatment plants"
                     /db_xref="taxon:2737536"
                     /environmental_sample
                     /geo_loc_name="Canada"
                     /lat_lon="49.25 N 123.1 W"
                     /collection_date="2015-06-16"
                     /metagenome_source="wastewater metagenome"
                     /note="metagenomic"
     gene            complement(88..900)
                     /locus_tag="PLF56_04745"
     CDS             complement(88..900)
                     /locus_tag="PLF56_04745"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_020392256.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_function: GO:0005524 - ATP binding [Evidence IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="ATP-binding cassette domain-containing protein"
                     /protein_id="HOQ52903.1"
                     /translation="MLHLVKDFRLRKGFRFSTLHAVQDVSLRIEPGRTVALVGESGSG
                     KSTIARILAKLETPTAGEVRLDGETTAFRGSQAHYRRQVQMVFQDPFASLNPFHSIAH
                     HIERPLKLHHPELSAVELRQRTVELLERVSLGAQYLTRRPHELSGGQRQRVAIARALA
                     PGARFIIADEPVSMLDVSIRVGVLNLLAELQRADGLGVLYITHDLATARHFSDEIMVM
                     LRGEIVERGPADQVILDPQHPYTASLREAAADPERLGQLRDEVRAEAAEGAR"
     gene            complement(1005..1997)
                     /locus_tag="PLF56_04750"
     CDS             complement(1005..1997)
                     /locus_tag="PLF56_04750"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_020392257.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_function: GO:0005524 - ATP binding [Evidence IEA];
                     GO_function: GO:0016887 - ATP hydrolysis activity
                     [Evidence IEA];
                     GO_function: GO:0042626 - ATPase-coupled transmembrane
                     transporter activity [Evidence IEA];
                     GO_function: GO:0140359 - ABC-type transporter activity
                     [Evidence IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="ABC transporter ATP-binding protein"
                     /protein_id="HOQ52904.1"
                     /translation="MSITYEVNPPVEAVREVSLTLNRGEILGLAGESGCGKTTFAYGI
                     NRLLKAPAVMTAGEVTFHDADGTDVDVVAMPAEALRRYRWSKVSMVFQGSMNSLNPVL
                     TVRTQLNDVFTTHRRGMSRRARNERAAELLSLVGVDPNRLSAYPHELSGGMRQRVMIA
                     MALALEPQVMIMDEPTTALDVLVQRGIIRQLMRLRERLGFAVIFITHDLPLLIEISDR
                     IAIMRAGRIVEQGTAEQIYRTPEHQYTKQLLSSFPSLTGARGGFVRGAGSGGTTSPEA
                     SAVVPAASPVVLAAASVIPAAPSVIPADAGISAAGTGIPGQARDDGFPARLEEE"
     gene            complement(2408..3322)
                     /locus_tag="PLF56_04755"
     CDS             complement(2408..3322)
                     /locus_tag="PLF56_04755"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_020392258.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_function: GO:0042626 - ATPase-coupled transmembrane
                     transporter activity [Evidence IEA];
                     GO_function: GO:0140359 - ABC-type transporter activity
                     [Evidence IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="ABC transporter permease"
                     /protein_id="HOQ52905.1"
                     /translation="MPQWSAKLGAGLVIVGLIVLFGLLGPLISFDPRDSSYRGLQPPS
                     SAHWLGTTKLGYDVFAQLAIGTRESLYVGLVGGLVAMGLAIVFGILAGYFGGWLDEGL
                     MLATNIMLVIPGLPLVMVIAAYVQNTDGLGDFARSSTLVALILGVTGWAGSAVVLRAQ
                     ARSLRTREYVAAARVAGESGLRIIFVEILPNLVPLIAAQLIFGGMAAVLGEAGLSYLG
                     LGPTNSITLGTMLNDAQTGQAVGSRAWWWYVPPGLMVALLGTGLSLINFAIDEIVDPK
                     LRLAPKAARQQRRATKAGKGVAGDGAFA"
     gene            complement(3450..4454)
                     /locus_tag="PLF56_04760"
     CDS             complement(3450..4454)
                     /locus_tag="PLF56_04760"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_020392259.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_process: GO:0055085 - transmembrane transport [Evidence
                     IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="ABC transporter permease"
                     /protein_id="HOQ52906.1"
                     /translation="MRFYLRRVAFYVITIWAAVSLNFLLPRMMPGDPAAIMIGKLRRA
                     SGGRELSPQVIQDIYALLGAGNTTSPWQQYLAYWTRLLHGDLGLSSTRYPYPVLELIG
                     NALPWTLFMVGLATVLSFVLGIAGGAFVGWKRGTWLDQLVPATSLLQSIPYFWLALVL
                     VAVFSVQLGWLPIVGAYDPFDFPSGPEWSWPFVASALQHAFLPALTIVISSVGGWLLG
                     MRNMMVSTMSEDYVLTAEAKGLKPSRIRDRYAARNAAIPSLAGFSIALAFVVAGSIVT
                     EQVFSYPGIGKLMIQSVQGLDYTLMQGVFLVITLTVLAANFAMDLLYGLIDPRVRNDI
                     "
     gene            complement(4489..6165)
                     /locus_tag="PLF56_04765"
     CDS             complement(4489..6165)
                     /locus_tag="PLF56_04765"
                     /inference="COORDINATES: protein motif:HMM:NF012706.2"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="ABC transporter substrate-binding protein"
                     /protein_id="HOQ52907.1"
                     /translation="MKPARKFAVAAVVLATALAGCTSPSTSPTTSPGASSAAPTSSRA
                     LRVWAGSTTPITIDFNPFHVDTALYGTYGPIYEPLFFFNQLKADPPTPMIGESYTYNA
                     DGKELTIKLRSGLKWSDGQPLTAADVVFTLGYGPNKTEHLLSAKATDDTTVVLTYDTP
                     QFTNASTLLGATMIVPKHVWSKVSNYMKETNSNPVGSGAYVVKTTSDASYTLTANSAY
                     RDPVPITNVQFLGIDSNQSGQDLLTTGQIDWTGMFVANPDSVTNTGAIATMNQQQDPT
                     TIITCADAAKGCKGDQTDPAVRQAINVAIDRSQLAEKAWVNLVGKANPAFVLLPRDSK
                     WLTDQSLAESPQSADIAKAAQILEAAGYTKDAKGFYGKGGKEINLDLFSPDGWTDYND
                     AAKLIAEQAGKAGIKITPRTVSEAEYWTPVTNGDFQMAMYGLTQSVVADPFSNYDQYF
                     NGKSTAKVGSAPVRGQNYARYSNPSVDAAVEAAAATNDEAIKKAEYAKIQAAIATDLP
                     YIPVVLNASQAFFNTKNFSGWPNDGDLYADPLPYLSVAPAIVLLRLKPLG"
     gene            6578..6817
                     /locus_tag="PLF56_04770"
     CDS             6578..6817
                     /locus_tag="PLF56_04770"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_014847117.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="CopG family transcriptional regulator"
                     /protein_id="HOQ52908.1"
                     /translation="MRTTVRLDPEVAAAAERLRRERHIGLGEAVNELARAGLGQKQRP
                     ARFRQRTASVGLKVDVTDVAGTLELLDQYDAEDAW"
BASE COUNT         1116 a         2248 c         2383 g         1134 t
ORIGIN      
        1 gctgggtgcg gctgagcggg ccgttcgcga gcaacagccg aaaggccgtc cggtcgttct
       61 gcgcgcgcaa ccaggccggg gtgcccttca tcgtgctcct tccgccgctt cggcccgcac
      121 ctcgtcgcgc agttgaccca gacgttccgg gtcggcggcg gcctcgcgca atgaggcggt
      181 gtaggggtgc tgcgggtcga gaatcacctg gtcggcgggg ccccgctcga cgatttcgcc
      241 acgcagcatg accatgatct catcagagaa atgccttgcc gtggccagat cgtgggtgat
      301 gtagagcacg cctagaccgt ccgcgcgttg tagctcggca agcaggttga gcaccccgac
      361 ccggatcgac acatcaagca tcgacaccgg ctcgtccgcg atgatgaacc gggcgcccgg
      421 cgcaagggcg cgggcgatcg cgactcgctg gcgttgcccg ccggacagtt cgtgcggacg
      481 ccgggtcagg tactgcgcgc cgagcgacac ccgctccagc agctcgaccg tgcgttgcct
      541 gagttcgacg gcggacagtt cggggtggtg cagcttcaac ggccgttcga tgtgatgggc
      601 gatgctgtga aacgggttca acgacgcgaa cggatcctga aacaccatct gcacctgccg
      661 ccggtagtgc gcctgcgagc cgcgaaaggc ggtggtttcg ccgtccagcc gcacctcgcc
      721 ggctgtgggc gtttcgagct tcgccagaat gcgcgcgatc gtcgacttac ctgagccgct
      781 ctcgccgacc agggccacgg tgcgtccggg ctcgatgcgc agggagacgt cctggacggc
      841 gtgcagggtg ctgaaccgga accccttgcg cagccggaag tccttgacga ggtggagcac
      901 ctccagaccg tccgccggtc gagatttccc gcccgccggt cgagctgcga catccgccgg
      961 tcgagttcct ccatccgtcg gtcgagcgtg tcgagacccg ctcatcactc ctcctcgagt
     1021 ctcgcgggga acccgtcatc ccgggcctgc cccgggatcc cagtcccggc ggcagagatt
     1081 ccggcgtccg ccggaatgac ggaaggagcc gccggaatga cagaagcagc cgccagcacg
     1141 acgggggacg ccgccggcac gacggcggaa gcctcgggcg aggtggtccc acccgacccg
     1201 gctccccgga cgaagccacc ccgggcgccg gtcaggctcg ggaagctgga gagcaactgc
     1261 ttggtgtact ggtgctccgg cgtccgatag atctgctcgg ccgtgccctg ctcgacgatc
     1321 cggccggcgc gcatgatcgc gatccggtcg ctgatctcga tcagcagcgg caggtcgtgg
     1381 gtgatgaaga tgacggcgaa cccgagtcgc tcgcgcaacc gcatgagttg ccggatgatg
     1441 ccgcgttgca ccagcacgtc gagggcggtg gtgggttcgt ccatgatcat cacctgcggc
     1501 tccagggcga gcgccatggc gatcatcacc cgttgtcgca tgccgcccga caactcgtgc
     1561 gggtaggccg agagccggtt ggggtcgacg ccgaccaggc tcaacagttc ggcggcacgc
     1621 tcgttgcggg cccgtcggct catgccgcgg cgatgcgtgg tgaacacgtc gttgagctgg
     1681 gtgcgcacgg tcagcaccgg attgagcgag ttcatcgagc cttgaaatac catcgagacc
     1741 ttgctccagc ggtagcggcg cagggcctcg gccggcatgg cgacgacgtc gacatcggtg
     1801 ccgtccgcgt catggaacgt gacctcgccg gcggtcatca ccgcgggcgc cttcagcaac
     1861 cggttgatgc cgtaggcgaa ggtggtcttg ccacagccgg attcgccggc caggcccagg
     1921 atctcgccgc ggttcagcgt cagcgacacc tcgcggacgg cctcgaccgg cgggttcacc
     1981 tcgtaggtga tcgacacgtt gcgagcggtc aggacggggg tgacgtcatc ccggcgaacg
     2041 tccccgtcat cccggcgaac gccgggatct cgcagccccg cgccgctcac gtcatcccgg
     2101 cgaaggtccc cgtcatcccg gcgaacgccg ggatctcgca acccgccgag cccgccgctt
     2161 cgctggccta gcgggtcgag accctgctcg gagatcccgg cgttcgccgg gatgacgggg
     2221 gctggccggg cgacgagggc tgacggaatg gcggcgccgg tggaatgctc ggcggccggg
     2281 ccggagatcc cggcgttcgc cgggatgacg gagtcggcgg ggccggagat cccggcgttc
     2341 gccgggatga cgggtgcatc cgggacgacg ggtgcttccg ggatgacggg tacaggagcc
     2401 tcgtcactca tgcgaaggct ccgtccccgg cgacgccctt accggccttc gtggcccggc
     2461 gttgctgccg ggccgccttg ggcgccagcc gcagcttggg gtcgacgatc tcgtcgatgg
     2521 cgaagttgat caacgacaac ccggtgccga gcagggcgac catcagaccc ggtggcacgt
     2581 accaccacca cgcccgcgac ccgaccgcct gcccggtctg ggcgtcgttg agcatcgtgc
     2641 ccaaggtgat cgagttggtc ggtccgagac ccagatagct cagcccggcc tcgcccagca
     2701 ccgccgccat acccccgaag atcagctgcg cggcgatcaa cggcaccagg ttcggcagaa
     2761 tctcgacgaa gatgatccgc agcccgctct cacccgccac ccgggccgcg gccacgtact
     2821 cacgggtgcg cagcgagcgt gcctgcgccc tgagcaccac cgccgagccg gcccagccgg
     2881 tcacgcccag aatcagtgcc actaaggtgg acgagcgcgc gaagtcgccc agcccgtccg
     2941 tgttctgcac gtaggcggcg atcaccatca ccagcggcag gcccggaatc accagcatga
     3001 tgttggtggc cagcatcaga ccctcgtcca gccagccgcc gaagtagccg gcgagaatgc
     3061 cgaacacgat cgccagcccc atcgcgacca gcccaccgac caggcccacg tacagcgatt
     3121 cgcgggtgcc gatggccagt tgcgcgaaca cgtcgtagcc cagcttcgtg gtgcccagcc
     3181 agtgtgcgga cgacggtggc tgcagacccc ggtaactcga atcgcgcggg tcgaagctga
     3241 tcagcggccc gagcaggccg aacaacacga tcaacccgac gatgaccagc ccggcgccca
     3301 gcttggccga ccactgcggc atcaggtggc gcagacctgc gaagcggtcg ggccgcgggc
     3361 tctcggccgt ggcgagcccc gacgtcccct gctcaactgc tcttcgagac ccccccggcc
     3421 ggctctgagc ccgctcgagc ggtggttgct cagatgtcat tgcgcaccct cggatcgatc
     3481 aggccgtaca gcaggtccat ggcgaagttg gcggcgagca cggtgagtgt gatgaccagg
     3541 aacacaccct gcatcagcgt gtagtcgagg ccctgcaccg actggatcat cagcttgccg
     3601 atgcccgggt agctgaacac ctgttcggtg acgatcgaac ccgccaccac gaacgccagc
     3661 gcgatgctga aacccgccag cgagggaatc gccgcgttgc gggccgcgta ccggtcgcgg
     3721 atccgggacg gtttgagccc cttggcctcg gcggtcagca cgtaatcctc gctcatcgtc
     3781 gacaccatca tgttgcgcat gcccagcagc cagccgccca ccgaactgat cacgatggtc
     3841 aacgccggca ggaacgcgtg ttgcagcgcg gacgccacga agggccagct ccactccgga
     3901 cccgacggaa agtcgaacgg gtcgtaggcc cccacgatcg gcagccagcc cagctgcacc
     3961 gagaacaccg ccaccagcac cagcgccagc cagaagtagg gaatcgattg cagcagggac
     4021 gtcgccggca ccaactggtc gagccaggtg ccgcgcttcc aacccacgaa ggcaccgccg
     4081 gcgatgccca gcacgaacga gagcacggtg gccaggccga ccatgaacag cgtccagggc
     4141 agggcgttgc cgatcaactc gagcaccggg tacgggtacc gggtggacga caggcccagg
     4201 tcaccgtgca gcagccgcgt ccagtaggcc aggtactgct gccacggcga ggtggtgttg
     4261 ccggcgccga gcagggcgta gatgtcctgt atcacctggg gggacaactc ccgtccgcca
     4321 ctggcgcgcc gcaacttgcc gatcatgatc gcggccgggt cgcccggcat catgcggggg
     4381 agcaagaagt tcagcgacac agctgcccag atcgtgatca cgtagaaggc gacgcgccgc
     4441 aggtagaacc gcatgaggcg aacctggctt cccgatcagc cctgccgttc agccgagcgg
     4501 cttcaggcgc agcagcacga tcgccggagc cacggacagg tacggcagcg ggtccgcgta
     4561 caggtcgcca tcgttcggcc agccgctgaa gtttttcgtg ttgaagaacg cctgggacgc
     4621 gttgagcacc accggaatgt agggcaggtc ggtcgcgatg gccgcctgga tcttggcgta
     4681 ctcggccttc ttgatcgcct cgtcgttggt ggcggcggcg gcctcgacgg cggcgtccac
     4741 gctcggattg ctgtagcgcg catagttctg gccgcggacg ggagcgctgc cgaccttggc
     4801 ggtggacttg ccgttgaagt actggtcata gttgctgaac gggtcggcga ccaccgattg
     4861 ggtcagtccg tacatggcca tttggaagtc gccgttggta acgggggtcc agtactcggc
     4921 ctcggagacg gtgcgcgggg tgatcttgat gcccgccttg ccggcctgtt cggcgatcag
     4981 tttggcggcg tcgttgtagt cggtccagcc gtcggggctg aacaggtcga ggttgatctc
     5041 tttgccgccc ttgccgtaga agcccttggc gtccttggtg tagccggcgg cctcgagaat
     5101 ctgggccgcc ttggcgatgt cggcgctctg cgggctctcg gcgaggctct ggtcggtcag
     5161 ccacttgctg tcgcgaggca gcagcacgaa ggccggattc gccttgccga ccaggttcac
     5221 ccacgccttc tcggcgagct gactacggtc gatggcaacg ttgatggcct gccgcacggc
     5281 cgggtcggtc tggtcgccct tgcagccctt ggcggcatcg gcgcaggtga tgatcgtggt
     5341 cgggtcctgc tgctggttca tggtggcgat cgccccggtg ttggtgaccg agtcggggtt
     5401 ggcgacgaac atgcccgtcc agtcgatctg gccggtggtg agcaggtcct gaccggactg
     5461 gttcgagtcg atgcccagga actgcacgtt ggtgatcggc accgggtcgc ggtaggcact
     5521 gttggcggtc agcgtgtacg acgcgtccga ggtggtcttc accacgtagg cgcctgagcc
     5581 gaccgggttg ctgttggtct ctttcatgta gttgctgacc ttcgaccaga cgtgcttcgg
     5641 cacgatcatg gtcgcgccga gcagggtgga cgcgttggtg aactggggcg tgtcgtaggt
     5701 gaggacgacc gtggtgtcgt cggtcgcctt ggccgagagc aggtgctcgg tcttgttggg
     5761 cccatagccg agggtgaaca ccacgtcggc ggcggtcagc ggctggccgt cgctccactt
     5821 cagcccgctc ctgagcttga tcgtgagctc tttgccgtcg gcgttgtagg tgtagctctc
     5881 accgatcatc ggcgtgggcg ggtcggcctt gagctggttg aagaagaaca gcggctcata
     5941 gatcggtcca taggtgccgt aaagagcggt gtcgacgtgg aagggattga agtcgatggt
     6001 gatcggcgtg gtgctgccgg cccagacccg taatgcgcgg gacgacgtgg gcgccgcgct
     6061 gctcgcgccg ggcgaggtgg tggggctcgt ggacgggctg gtgcagccgg ccagcgcggt
     6121 ggcgagaacg acggctgcga cggcgaactt tcgagctggc ttcatggttc ggctcccagg
     6181 ttgtggcgac ctccggcgac ggtgccggca ggcccacgct aaccgaaaac aaactattcg
     6241 gaaagactct ttctggttac gtttcggtat cggcgtcgcg cgcagtgtct ggttggtcct
     6301 gggcacaggg attgggttcg gtgccgtttg ctggcgacgg ctccgccggc aaacggcagc
     6361 ctcgatcggt gccttggctg gagctgggcc gcggccgcag gggacccggc taacgttcga
     6421 cgctgggcat cgggcgagac ccaggagcat gtcggctcat gcagtgtgca tgtggacggt
     6481 ccgcgacatc gcgtgctagt cggccacgaa gaccacattc acctggccta gcatcactga
     6541 tgcggacaga cagcatctgt ggtgctaccg tagcggcatg cggacaacgg tacgactcga
     6601 ccccgaggtc gcggcggcgg cggaacgcct gcgcagggag cgtcacatcg gtctgggtga
     6661 ggctgtcaac gagctcgccc gcgcgggtct gggccagaag cagaggcctg cgcgttttcg
     6721 gcaacgcacg gccagcgtcg ggctgaaagt cgatgtcacc gacgtcgccg gcaccctgga
     6781 gttgctcgat cagtacgacg ccgaggatgc ctggtgatcg tcgacgccaa cgtcctgctc
     6841 tacgccgtcg atgaccagtc ttatttccac accaccgccc g
//
LOCUS       DAOSUG010000101         6846 bp    DNA     linear   ENV 10-JUN-2024
DEFINITION  MAG TPA_asm: Micropruina sp. isolate AMR_MDS_2195
            NODE_5381_length_6846_cov_4.125607, whole genome shotgun sequence.
ACCESSION   DAOSUG010000101 DAOSUG010000000
VERSION     DAOSUG010000101.1
DBLINK      BioProject: PRJNA925477
            BioSample: SAMN32864262
            Sequence Read Archive: SRR4236673
KEYWORDS    WGS; ENV; Metagenome Assembled Genome; MAG; Third Party Data; TPA;
            TPA:assembly.
SOURCE      Micropruina sp. (wastewater metagenome)
  ORGANISM  Micropruina sp.
            Bacteria; Bacillati; Actinomycetota; Actinomycetes;
            Propionibacteriales; Nocardioidaceae; Micropruina.
REFERENCE   1  (bases 1 to 6846)
  AUTHORS   Abdulkadir,N., Saraiva,J.P., Zhang,J., Stolte,S., Gillor,O.,
            Harms,H. and Rocha,U.
  TITLE     Genome-centric analyses of 165 metagenomes show that mobile genetic
            elements are crucial for the transmission of antimicrobial
            resistance genes to pathogens in activated sludge and wastewater
  JOURNAL   Microbiol Spectr 12 (3), e0291823 (2024)
   PUBMED   38289113
REFERENCE   2  (bases 1 to 6846)
  AUTHORS   Abdulkadir,N.
  TITLE     Direct Submission
  JOURNAL   Submitted (24-MAR-2023) Environmental Microbiology, Helmholtz
            Center for Environmental Research -UFZ, Permoserstr 15, Leipzig
            04318, Germany
COMMENT     The annotation was added by the NCBI Prokaryotic Genome Annotation
            Pipeline (PGAP). Information about PGAP can be found here:
            https://www.ncbi.nlm.nih.gov/genome/annotation_prok/
            
            ##Genome-Assembly-Data-START##
            Assembly Method        :: SPAdes v. 3.11.1
            Genome Representation  :: Full
            Expected Final Version :: Yes
            Genome Coverage        :: Not Applicable
            Sequencing Technology  :: Illumina MiSeq
            ##Genome-Assembly-Data-END##
            
            ##Genome-Annotation-Data-START##
            Annotation Provider               :: NCBI
            Annotation Date                   :: 05/04/2023 04:57:10
            Annotation Pipeline               :: NCBI Prokaryotic Genome
                                                 Annotation Pipeline (PGAP)
            Annotation Method                 :: Best-placed reference protein
                                                 set; GeneMarkS-2+
            Annotation Software revision      :: 6.5
            Features Annotated                :: Gene; CDS; rRNA; tRNA; ncRNA
            Genes (total)                     :: 2,776
            CDSs (total)                      :: 2,750
            Genes (coding)                    :: 2,714
            CDSs (with protein)               :: 2,714
            Genes (RNA)                       :: 26
            tRNAs                             :: 24
            ncRNAs                            :: 2
            Pseudo Genes (total)              :: 36
            CDSs (without protein)            :: 36
            Pseudo Genes (ambiguous residues) :: 0 of 36
            Pseudo Genes (frameshifted)       :: 2 of 36
            Pseudo Genes (incomplete)         :: 32 of 36
            Pseudo Genes (internal stop)      :: 2 of 36
            ##Genome-Annotation-Data-END##
FEATURES             Location/Qualifiers
     source          1..6846
                     /organism="Micropruina sp."
                     /mol_type="genomic DNA"
                     /submitter_seqid="NODE_5381_length_6846_cov_4.125607"
                     /isolate="AMR_MDS_2195"
                     /isolation_source="Activated sludge of municipal
                     wastewater treatment plants"
                     /db_xref="taxon:2737536"
                     /environmental_sample
                     /geo_loc_name="Canada"
                     /lat_lon="49.25 N 123.1 W"
                     /collection_date="2015-06-16"
                     /metagenome_source="wastewater metagenome"
                     /note="metagenomic"
     gene            <1..282
                     /locus_tag="PLF56_04775"
     CDS             <1..282
                     /locus_tag="PLF56_04775"
                     /inference="COORDINATES: protein motif:HMM:NF019349.2"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="FCD domain-containing protein"
                     /protein_id="HOQ52909.1"
                     /translation="FNELDTDFHVAMAEAAHNSLITEMTTAIRHSMRTLVLASFREHG
                     HWPELSDQLRAQHRTIFDAIAAGDQKTAADAVEEHLRSANEALGWGASD"
     gene            443..1093
                     /locus_tag="PLF56_04780"
     CDS             443..1093
                     /locus_tag="PLF56_04780"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_014846359.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_function: GO:0003677 - DNA binding [Evidence IEA];
                     GO_process: GO:0000160 - phosphorelay signal transduction
                     system [Evidence IEA];
                     GO_process: GO:0006355 - regulation of DNA-templated
                     transcription [Evidence IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="response regulator transcription factor"
                     /protein_id="HOQ52910.1"
                     /translation="MAASIRVLVVDDDPVVRAAYRTLLDREPDLVLVGEVPDGSGAVE
                     AYEATRPDVVLMDLQMPRVSGIDATAALCERWPEAVVVVMTTFSTREHVVAALRAGAA
                     GYLVKGVPATALAVALRQALAGDMPLASAVRRELVATLTPAPAPPQHQLTPRETELVQ
                     RLAQGLSNAQIGRRMHLSEGSVKQYLARIGDKWGLRSRTQILIRGIQLGVVDPDAQ"
     gene            complement(1098..3416)
                     /locus_tag="PLF56_04785"
     CDS             complement(1098..3416)
                     /locus_tag="PLF56_04785"
                     /inference="COORDINATES: protein motif:HMM:NF019350.2"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_component: GO:0016020 - membrane [Evidence IEA];
                     GO_function: GO:0000155 - phosphorelay sensor kinase
                     activity [Evidence IEA];
                     GO_function: GO:0046983 - protein dimerization activity
                     [Evidence IEA];
                     GO_process: GO:0000160 - phosphorelay signal transduction
                     system [Evidence IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="histidine kinase"
                     /protein_id="HOQ52911.1"
                     /translation="MLPIATLRTGRTKLSRPLQIVRALVIVLVVSDLVSAPQVPAPQP
                     WGYALPIASAFASIVALFRPGLGVLLALLPIGSTLVVGPWGQDFMPLLVTTVMICVIG
                     SRWWVAWTLGLYLAYVLAVARLDTGERALTCLSAIVVAALGGFIVRGVVAQARRRDLS
                     IERVRVQTVLLRATERRALADELSQLLKDALAGQGLTQSRAAGSNDPAELRLALAQAE
                     VNAVDTLAQLRGLVSTLRGRGDIAARVDAGAPGLLNLVEEAEDLLVGNGHPVEVELPE
                     SIEVAGDLVPRLLGQILREAAPLMARQGAPGQPCRIEVTVEPEVVRVLLSHPCERVDD
                     TPAGLLAAGERVIAAGGTFGIVQSAGRWSLAVSLPLLSQAVAESAPDRGASAVAVKPA
                     RMRAVGALVLLIAGAAALGVAVQDPLNGHAGGAWWGLWALLLLACGLATWLRSLPVAV
                     TGLAAVSLIAAWTIDATQVIGQPNQVAIMVLMAIAVGRDSRAAWAIPPYWIGCCLLWA
                     GGFQLGFVTPQLLHVVLGLMLGLVIAFFRNLRAVQRDRLLAVTSTHEEAQGEVRRELA
                     GELHDIVAHQLSLITLQAGAHRHDTELVTLRGALDQVASITRSAQADLALLLHVMRSS
                     SSAADDASAVWLAPRPAAEAAARTLREAGHGVALDVDESVEGLDPTTRKTLTRLIREG
                     TTNVLRYAPSGSSCTLRVRREGAEVRFEMTNPVAERSVRSRHSTGLGLVGLDERVRIT
                     GGRLTAGTFEGRWRLDVTLPATPAVDLRPAPT"
     gene            complement(3656..4198)
                     /locus_tag="PLF56_04790"
     CDS             complement(3656..4198)
                     /locus_tag="PLF56_04790"
                     /inference="COORDINATES: ab initio
                     prediction:GeneMarkS-2+"
                     /note="Derived by automated computational analysis using
                     gene prediction method: GeneMarkS-2+."
                     /codon_start=1
                     /transl_table=11
                     /product="hypothetical protein"
                     /protein_id="HOQ52912.1"
                     /translation="MSAINSTGALRRTLGTVERSTSAALTELRLLAEVLRHDPSTAAN
                     GMKVRDLSRWQPPTQACAEAQLDLVRAGLEPQVNVPAAADSLAMTVQRTLSRLVKEAV
                     ANQVAHAPSGALCTIDVDVAEHQVTLRAANAMPQGSDGEATLGWGLLGLRERVTLTGG
                     DFAVGQDGPDWVLTATLPRE"
     gene            4318..4443
                     /locus_tag="PLF56_04795"
     CDS             4318..4443
                     /locus_tag="PLF56_04795"
                     /inference="COORDINATES: ab initio
                     prediction:GeneMarkS-2+"
                     /note="Derived by automated computational analysis using
                     gene prediction method: GeneMarkS-2+."
                     /codon_start=1
                     /transl_table=11
                     /product="hypothetical protein"
                     /protein_id="HOQ52913.1"
                     /translation="MSDALGLQYDLPDTPDEEKASRISIVACTSSHASYFLCYQK"
     gene            4484..4618
                     /locus_tag="PLF56_04800"
     CDS             4484..4618
                     /locus_tag="PLF56_04800"
                     /inference="COORDINATES: ab initio
                     prediction:GeneMarkS-2+"
                     /note="Derived by automated computational analysis using
                     gene prediction method: GeneMarkS-2+."
                     /codon_start=1
                     /transl_table=11
                     /product="hypothetical protein"
                     /protein_id="HOQ52914.1"
                     /translation="MADILDLQNSDAEPTPGEEKASNISVRNFFCRNSFVSVSICFTK
                     "
     gene            4662..4790
                     /locus_tag="PLF56_04805"
     CDS             4662..4790
                     /locus_tag="PLF56_04805"
                     /inference="COORDINATES: ab initio
                     prediction:GeneMarkS-2+"
                     /note="Derived by automated computational analysis using
                     gene prediction method: GeneMarkS-2+."
                     /codon_start=1
                     /transl_table=11
                     /product="class III lanthipeptide"
                     /protein_id="HOQ52915.1"
                     /translation="MADILNLQQEETPEVPGEEKGSNNSYFICHNSNASNFLCWRP"
     gene            5008..5790
                     /locus_tag="PLF56_04810"
     CDS             5008..5790
                     /locus_tag="PLF56_04810"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_016668178.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_function: GO:0005524 - ATP binding [Evidence IEA];
                     GO_function: GO:0016887 - ATP hydrolysis activity
                     [Evidence IEA];
                     GO_function: GO:0042626 - ATPase-coupled transmembrane
                     transporter activity [Evidence IEA];
                     GO_function: GO:0140359 - ABC-type transporter activity
                     [Evidence IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="ABC transporter ATP-binding protein"
                     /protein_id="HOQ52916.1"
                     /translation="MVSQIREPRTADTAPTGVAIAAHDLRKAYQVGQQSLEILHGISL
                     SVSSGELVAVMGPSGSGKSTLMYCLAGLEAPTGGHVQLAGRPLERLSRADLARMRRTS
                     VGFVFQSYNLIPTLTAAENVALPYLLAGRKPAAGLVEQTLEAVGLAQRATARPPSMSG
                     GEQQRVALARVLAQQPEVIFADEPTGALDTRTGELVLAELRRIAHSPKGCVLVVTHDP
                     SVAAACDRTLFLRDGQLVQELRGASVDVVAATLARLGARSGG"
     gene            5793..>6846
                     /locus_tag="PLF56_04815"
     CDS             5793..>6846
                     /locus_tag="PLF56_04815"
                     /inference="COORDINATES: ab initio
                     prediction:GeneMarkS-2+"
                     /note="Derived by automated computational analysis using
                     gene prediction method: GeneMarkS-2+."
                     /codon_start=1
                     /transl_table=11
                     /product="hypothetical protein"
                     /protein_id="HOQ52917.1"
                     /translation="MLRIYRAELRDSWPAWLSVSLTFIMVNAALALTIIVGFTGLAAM
                     AAGRLSFENSAAWTIGQALVLLAIVLVALPVVGSATSLVVASRRGGLARLALAGATPA
                     QVRGAISAQLAVVSLLCAPMGDALAVFATSPWLALMDYQARNEPGWVSLPPNFAPLPI
                     IAANLICSLLVVWAGSRQARVASEIPPVEALRQSQAPERRTRLKPGGWAVAILGAGLV
                     AGSFLSVQVQLEHRYKETVSNLLILGFMQVYLWGGLLATLAPVLVGPLTRCWTALVPT
                     SSGSWQLARATVSARVDRLYKSVVPVMFTFALGVGSLSVVDSMIATMAVSMGRLDLAV
                     PMWDTFVLQFGLPLVIA"
BASE COUNT         1074 a         2482 c         2299 g          991 t
ORIGIN      
        1 ttcaacgaac tcgacaccga ctttcacgtg gcgatggccg aggccgccca caacagcctg
       61 atcaccgaga tgaccaccgc gatccggcac tcgatgcgca ccctcgtgct cgcctcgttc
      121 cgcgaacacg ggcactggcc cgaactctcc gaccaactgc gcgcccagca ccgcacgatc
      181 ttcgacgcca tcgccgcggg cgaccagaag actgccgccg acgccgtcga ggagcacctg
      241 cgcagcgcga acgaggcgct gggctggggc gcctccgatt gaggccggcg gggttccgtc
      301 ggctctgctg gttggacgca cccccgggac gcacccgccg ccctttggtg gttgcgaata
      361 caccggagcc cgtcgccccc aggcacgagc tctcgacaag gcgtgaacag cggagccgca
      421 aacggccgga tatcctgccg gggtggccgc ttcgattcgc gtcctggtcg tcgacgacga
      481 cccggtcgtg cgcgccgcgt accgcacgct gctcgaccgg gagcccgacc ttgtcctggt
      541 cggcgaggtg ccggacggat cgggcgcggt cgaggcctac gaggccaccc gtcccgacgt
      601 ggtgctgatg gatctgcaga tgccccgggt ctcgggcatc gacgccaccg ccgccctctg
      661 cgagcgctgg cccgaggcgg tcgtggtggt gatgaccacc ttcagcaccc gtgagcatgt
      721 ggtggcggcg ctgcgcgccg gggcggccgg ctacctggtc aaaggggtgc ccgcgaccgc
      781 tctggccgtc gccctgcggc aggcgcttgc gggcgacatg ccgctggcgt cggcggtgcg
      841 ccgtgagctg gtcgccaccc tgaccccggc accggcgcca ccgcagcacc aactcacccc
      901 gcgcgagacc gagttggtgc agcggctggc ccagggcctc agcaacgccc agatcggacg
      961 ccgtatgcac ctgtccgagg ggtcggtgaa gcagtatctg gcccgaatcg gcgacaagtg
     1021 gggcctgcgc tcacgtaccc agatcctgat tcgcggtatt cagctcggcg tggtcgaccc
     1081 cgacgcccag tgatacctca cgtgggcgcg gggcgcaggt cgacggccgg ggtggccggc
     1141 agcgtcacgt ccaaccgcca gcggccctcg aacgtaccgg cggtcagccg tcccccggtg
     1201 atccgcaccc gctcgtccag cccgacgagg ccgagtccgg tcgagtgccg ggagcgcacg
     1261 gaacgctcgg cgaccgggtt ggtcatctcg aaccgcacct cggcaccttc ccgacgcacg
     1321 cgtagcgtgc acgacgagcc gctgggcgca tagcgcaaca cgttggtggt gccctcacgg
     1381 atcaaccggg tgagggtctt gcgggtggtc gggtcgaggc cctcgacgga ttcgtcgacg
     1441 tcgagcgcga ccccatgccc ggcctcgcgc aaggtcctcg cggcggcctc ggcggccggc
     1501 ctcggggcca accagaccgc cgacgcgtcg tccgcagcgc tggacgagga ccgcatcacg
     1561 tggagcagca gcgcgaggtc ggcctgggcc gagcgggtga tcgacgcgac ctgatcgagt
     1621 gcgccgcgca gcgtgaccag ctcggtgtcg tggcggtgcg cccccgcttg caacgtgatc
     1681 agcgagagtt gatgcgccac gatgtcatgc agctcacccg ccagttcgcg tcgaacctcg
     1741 ccctgagctt cttcgtgggt gctggtgacg gccagcagcc gatcgcgctg cacagcacgc
     1801 aggttgcgga agaaggcgat caccaaaccg agcatcaagc ccaacaccac gtgcaacagt
     1861 tgcggagtga cgaagccgag ttggaacccc ccggcccaca gcaggcagca cccgatccag
     1921 tacggcggaa tcgcccacgc cgcacgcgag tcgcgcccca ccgcgatggc catcagcacc
     1981 atgatcgcca cctggttggg ctgaccgatc acctgggtgg cgtcaatcgt ccaggcggcg
     2041 atcagcgaca ccgccgccag gccggtcacc gcgaccggca gggaacgcag ccaggtcgcc
     2101 aggccgcagg ccagcagcag cagggcccac agcccccacc aagcgccgcc ggcgtggccg
     2161 ttgagagggt cctgcacggc gactcccaag gcagctgcgc cggcgatcag caggaccagc
     2221 gcccctacgg cccgcatccg cgccggcttc accgccacgg cactcgcgcc ccggtcgggc
     2281 gccgattctg cgaccgcctg gctgagtagc ggcaaagaga ccgccaatga ccaccgcccc
     2341 gcactctgga cgatgccgaa cgttccgccg gcggcgatca cccgctcgcc cgcggccagc
     2401 agcccggcag gggtgtcatc gacgcgctcg cagggatgac tcaacagcac acgcaccacc
     2461 tcgggctcga ccgtgacctc gatgcggcag ggttgcccgg gcgcaccctg gcgcgccatc
     2521 aacggcgcag cctcgcgcag aatctgaccc agcagccgag gcaccagatc gccggcgacc
     2581 tcgatgctct cgggcagttc gacctcgacc gggtggccgt tgccgaccag caggtcttcg
     2641 gcctcctcga ccaggttcag cagtccgggt gcgcccgcat cgacccgggc ggcgatgtcg
     2701 ccgcgaccgc gcagcgtcga gaccaacccc cgcagttggg ccagcgtgtc gacagcgttg
     2761 acctcggcct gcgccagggc gagccgcagt tccgccgggt cgttactgcc cgcggcccgg
     2821 gactgcgtga gcccctggcc ggccagggcg tccttcagca actgtgacag ctcgtccgcc
     2881 aaggcgcggc gttcagttgc cctcagcagc accgtctgga cgcgcacccg ttcgatgctc
     2941 aggtcgcgcc gacgcgcttg ggcgacgacc ccgcgaacga tgaagccacc cagagcggcg
     3001 acgacgatcg cgctcaggca ggtcagtgcc cgctccccgg tgtccagtcg ggccaccgcg
     3061 agcacgtagg ccaggtagag cccgagcgtc caggccaccc accaacgcga gccgatcacg
     3121 cagatcatga cggtggtcac cagcaggggc atgaagtcct gaccccacgg accgaccacc
     3181 agggtcgaac cgatcggcag cagcgccagc agcacgccca ggccgggtcg gaacaacgcg
     3241 acgatgctcg cgaaggccga ggcgataggc aacgcgtacc cccacggctg cggggccggc
     3301 acctgcggag cggacaccag atccgacacg accagcacga tgacgagcgc acggacgatc
     3361 tgcaagggcc ggctcagctt ggtccgaccc gtccgcagcg ttgcgatcgg gagcatgggg
     3421 tgagatttcc tggagtgggg tggcaccact gtagcccgcc caagcccaga tccagcgagt
     3481 ggcgccgacg cgagcggcac cacacgggtg ggccggcaag ccggtccggg cgggggcatc
     3541 ccgtgcgcct gccctgcgtc acccgcgaag ccagggcgtc cggatggggt cgcgctggag
     3601 agcgatggat ccgggctgac ggcgggcgcg tcgctgtgcg tggtggacac ggggctcact
     3661 cgcgaggcaa ggtggcggtg agcacccaat ccggaccgtc ctggccgacg gcaaagtcac
     3721 caccggtgag cgtcacccgc tcccgcaacc ccaggagccc ccagcccagg gtggcctcac
     3781 cgtccgaacc ctgcggcatc gcgttcgcag cgcgcaacgt gacctgatgc tcggccacgt
     3841 cgacgtcgat cgtgcacagc gccccggacg gggcgtgcgc gacctgattg gcgacggcct
     3901 ctttgaccag tcgcgacagg gtgcgttgca cggtcatcgc cagcgagtcg gcggccgccg
     3961 gcacgttcac ctggggttcg aggcccgctc gcaccagatc gagttgggcc tcagcacagg
     4021 cctgtgtcgg cggctgccag cgggacaggt cgcgcacctt catgccattg gccgcggtgg
     4081 acgggtcgtg gcgcaacacc tcggccagca gccgcagctc ggtgagcgcg gcgctggtcg
     4141 aacgctcgac cgtgcccaac gtacgccgca acgccccggt cgagttgatt gcggacactc
     4201 ccgccgacac ctaccgctcg ggaaacagcg tgttcacctg cctggcccgc agcggtttca
     4261 gcatgggctg ctgacgcagc ccaccggttt cacgacaaca gagaggaggt gaatccgatg
     4321 agcgacgccc tcggtctgca gtacgacctg cccgacactc ccgacgaaga gaaggcttcg
     4381 aggatcagca tcgtggcctg caccagcagc cacgccagct acttcttgtg ctaccagaag
     4441 tgaccgcagc ccaccccacg acccagagag gaggtgacca cccatggccg acattctcga
     4501 cctgcagaac agcgacgccg agcccacccc cggtgaagag aaggcgtcga acatcagcgt
     4561 ccgcaacttc ttctgccgca actccttcgt gtccgtttcg atctgcttca cgaagtgagc
     4621 ccgccgcccg ccaccccgaa ccctcaaagg aggtgaacac catggcagac atcctgaacc
     4681 tgcagcagga ggagaccccc gaggtccccg gcgaagagaa gggctcgaac aacagctact
     4741 tcatttgcca caactccaac gcgagcaact tcctgtgctg gcgtccgtga gcaatcacga
     4801 caccgtgtga ttgcggacct ggccggcgcc tatccgacca gacggcaaac gaaagaccgc
     4861 caccccaccc cgggtggcgg tctttcgtcg cgtcgagccc gcgctcggcg cggcgttcac
     4921 taacggtcag tttcgccccg ccgccgcaga cttcgcccgc ctcgggtgct gaactggaac
     4981 ggtcctaacc catcccttgg aggaatcatg gtgagtcaga tccgcgaacc gcggacggcc
     5041 gacaccgcac cgactggtgt cgcgatcgcg gcccacgacc tgcggaaggc gtatcaggtc
     5101 ggccagcaga gcctggagat cctgcacggc atcagcttga gcgtttcgag cggcgaactg
     5161 gtggccgtga tgggcccgtc cggctccggc aaatcgaccc tgatgtactg cctggccggc
     5221 ttggaggccc cgaccggcgg ccacgtccag ttggccggac gtcccctgga gcgactctca
     5281 cgcgccgacc tggcccggat gcggcgcacg agcgtcggct tcgtctttca gtcgtacaac
     5341 ctgatcccca cgctgaccgc ggccgagaac gtcgccctgc cctatctgct ggccggacgc
     5401 aagccggccg ccggtctggt cgaacagacc ttggaggcgg tcggcctggc gcagcgcgcc
     5461 actgcccgtc cgccctccat gtcgggcggc gagcagcaac gggtggctct ggcccgcgtg
     5521 ctggcccaac agccggaggt gatcttcgcc gacgagccca ccggcgcgct ggatacccgc
     5581 accggcgagt tggtgctggc cgaactacgt cgcatcgccc actccccgaa ggggtgcgtg
     5641 ctggtggtca cccacgatcc gagcgttgcc gcggcctgcg accgcacgct gttcctacgc
     5701 gacgggcaac tcgtgcaaga gctgcgcggt gcctcggtcg acgtggtggc ggccaccctg
     5761 gcaaggctcg gcgcccgctc cggaggctga tcatgctgcg gatctatcgc gccgagttgc
     5821 gggactcgtg gcccgcctgg ctctcggtgt cgctcacctt catcatggtg aacgcagccc
     5881 tggccctgac gatcatcgtc ggcttcaccg gcctggccgc gatggccgct ggccggctca
     5941 gttttgagaa ctcggccgca tggacgatcg gccaggccct ggtgctgctc gccatcgtgc
     6001 tggtggccct gcccgtggtc ggctcggcca cctcgctcgt ggtcgcctcg cggcgtggcg
     6061 ggctcgcccg gctggcgctc gccggggcca ccccggcgca ggtgcgtggc gcgatctcgg
     6121 ctcaactggc cgtggtgtcg ctgctgtgcg cgccgatggg tgatgcgctg gctgtgttcg
     6181 cgacatcccc gtggcttgcg ctgatggact accaggcgcg caacgagccg gggtgggtgt
     6241 cattgccgcc caacttcgct ccgctgccga tcatcgccgc caacctgatc tgctcgttgc
     6301 tggtggtctg ggcgggcagc cgccaggccc gcgtggccag cgagattcca cccgtcgagg
     6361 cgctgcgcca gtcgcaggcg cccgagcgcc ggacccgcct gaagcccggc ggctgggccg
     6421 tggcgatcct cggcgcgggg ctggtcgcgg ggtcgttcct ctcggtgcag gtgcaactgg
     6481 agcaccggta caaagagacg gtcagcaacc tgctgatcct cggtttcatg caggtctacc
     6541 tgtggggcgg cctcctcgcc acgctcgcgc ccgtgctggt cggcccactg acccggtgct
     6601 ggacggcgtt ggtacccacc tcgtccgggt cgtggcagct ggcgcgggcc accgtctcgg
     6661 cacgggtcga ccggctgtac aagtcggtcg tgccggtgat gttcacgttc gccctcggcg
     6721 tggggtcact cagcgtcgtc gattcgatga tcgccacgat ggccgtctcg atgggccgcc
     6781 tcgacctggc cgtgccgatg tgggacacct tcgtgctgca gttcggcctg cccctggtga
     6841 tcgcct
//
LOCUS       DAOSUG010000102         6812 bp    DNA     linear   ENV 10-JUN-2024
DEFINITION  MAG TPA_asm: Micropruina sp. isolate AMR_MDS_2195
            NODE_5406_length_6812_cov_4.328548, whole genome shotgun sequence.
ACCESSION   DAOSUG010000102 DAOSUG010000000
VERSION     DAOSUG010000102.1
DBLINK      BioProject: PRJNA925477
            BioSample: SAMN32864262
            Sequence Read Archive: SRR4236673
KEYWORDS    WGS; ENV; Metagenome Assembled Genome; MAG; Third Party Data; TPA;
            TPA:assembly.
SOURCE      Micropruina sp. (wastewater metagenome)
  ORGANISM  Micropruina sp.
            Bacteria; Bacillati; Actinomycetota; Actinomycetes;
            Propionibacteriales; Nocardioidaceae; Micropruina.
REFERENCE   1  (bases 1 to 6812)
  AUTHORS   Abdulkadir,N., Saraiva,J.P., Zhang,J., Stolte,S., Gillor,O.,
            Harms,H. and Rocha,U.
  TITLE     Genome-centric analyses of 165 metagenomes show that mobile genetic
            elements are crucial for the transmission of antimicrobial
            resistance genes to pathogens in activated sludge and wastewater
  JOURNAL   Microbiol Spectr 12 (3), e0291823 (2024)
   PUBMED   38289113
REFERENCE   2  (bases 1 to 6812)
  AUTHORS   Abdulkadir,N.
  TITLE     Direct Submission
  JOURNAL   Submitted (24-MAR-2023) Environmental Microbiology, Helmholtz
            Center for Environmental Research -UFZ, Permoserstr 15, Leipzig
            04318, Germany
COMMENT     The annotation was added by the NCBI Prokaryotic Genome Annotation
            Pipeline (PGAP). Information about PGAP can be found here:
            https://www.ncbi.nlm.nih.gov/genome/annotation_prok/
            
            ##Genome-Assembly-Data-START##
            Assembly Method        :: SPAdes v. 3.11.1
            Genome Representation  :: Full
            Expected Final Version :: Yes
            Genome Coverage        :: Not Applicable
            Sequencing Technology  :: Illumina MiSeq
            ##Genome-Assembly-Data-END##
            
            ##Genome-Annotation-Data-START##
            Annotation Provider               :: NCBI
            Annotation Date                   :: 05/04/2023 04:57:10
            Annotation Pipeline               :: NCBI Prokaryotic Genome
                                                 Annotation Pipeline (PGAP)
            Annotation Method                 :: Best-placed reference protein
                                                 set; GeneMarkS-2+
            Annotation Software revision      :: 6.5
            Features Annotated                :: Gene; CDS; rRNA; tRNA; ncRNA
            Genes (total)                     :: 2,776
            CDSs (total)                      :: 2,750
            Genes (coding)                    :: 2,714
            CDSs (with protein)               :: 2,714
            Genes (RNA)                       :: 26
            tRNAs                             :: 24
            ncRNAs                            :: 2
            Pseudo Genes (total)              :: 36
            CDSs (without protein)            :: 36
            Pseudo Genes (ambiguous residues) :: 0 of 36
            Pseudo Genes (frameshifted)       :: 2 of 36
            Pseudo Genes (incomplete)         :: 32 of 36
            Pseudo Genes (internal stop)      :: 2 of 36
            ##Genome-Annotation-Data-END##
FEATURES             Location/Qualifiers
     source          1..6812
                     /organism="Micropruina sp."
                     /mol_type="genomic DNA"
                     /submitter_seqid="NODE_5406_length_6812_cov_4.328548"
                     /isolate="AMR_MDS_2195"
                     /isolation_source="Activated sludge of municipal
                     wastewater treatment plants"
                     /db_xref="taxon:2737536"
                     /environmental_sample
                     /geo_loc_name="Canada"
                     /lat_lon="49.25 N 123.1 W"
                     /collection_date="2015-06-16"
                     /metagenome_source="wastewater metagenome"
                     /note="metagenomic"
     gene            complement(<1..476)
                     /locus_tag="PLF56_04820"
     CDS             complement(<1..476)
                     /locus_tag="PLF56_04820"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_013864951.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_function: GO:0015267 - channel activity [Evidence IEA];
                     GO_process: GO:0055085 - transmembrane transport [Evidence
                     IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="MIP/aquaporin family protein"
                     /protein_id="HOQ52918.1"
                     /translation="MTLATIFLSETVGTAMLLLLGAGVVANNILPKNKGEGTGFLMVN
                     FGWGLAVFAGVLVSYKSGGHLNPAVTLGIVASGATDFVPGIPVNFASILTYIGAQMVG
                     AFIGAVLAWLAYKKQFDGDAPEGFKLGVFSTGPEVRDTVWNLVTEAVATFVLVFVVL"
     gene            720..1670
                     /locus_tag="PLF56_04825"
     CDS             720..1670
                     /locus_tag="PLF56_04825"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_002522776.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_function: GO:0030246 - carbohydrate binding [Evidence
                     IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="sugar-binding domain-containing protein"
                     /protein_id="HOQ52919.1"
                     /translation="MNARYDDMYQAASRYYIQGETMDAIAKQLGLSRSTVSRLLKDAR
                     ASGLVRITLADHTGSRSPLAGALVKTFGVRVHLVTVRDSLSDYRRFDQVARLAGRLLS
                     EAVADQCTIGIAWGVTASAVVRYLQPKPLVGSTVVQLNGGTNQWGSGIPYVGEILQSV
                     CDAFSSRVVYFPVPAFFDYAETKSAMWRERSVQNVVGLQRRTDVAVFGVGCLRARVPS
                     HVYSAGYLDDDDKRALSADGVVGDVATVLLREDGSWKDIGFNERASGLTPAELARVPR
                     RFCVVADPSRAAAVVGALRAGTTTDLVIDNATALAVLERL"
     gene            1670..3565
                     /locus_tag="PLF56_04830"
     CDS             1670..3565
                     /locus_tag="PLF56_04830"
                     /inference="COORDINATES: protein motif:HMM:NF025182.2"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="DUF4185 domain-containing protein"
                     /protein_id="HOQ52920.1"
                     /translation="MPRPRAVIGPASLLESADRPAWWTPPGRPHRLEALIGDPQADGT
                     LRIEHWFCGQHRPDNPWVRGDAISDRALAPAALTQRGRPGTAAHELVAHIPEADGLIE
                     YAWAVNQSSPMRWRRVGAAPAVPAPRRPAWDGPEHAAEWGGGTVIATATAATRLRSGW
                     VQALVQVDDSLYHLHRQQHGQRVRWVRHACLRLRDHDAFEVAPEPSVKLAQVTGERDS
                     QPRPGSTGATLSSSESVAGVRGTDLGVRVEADGRSFLLFGDTHWTRPWLGTRDSIAEV
                     RDASAERPDVVFHGSPLSIRGGGTTLREYDVPLDAFSLEGRQYAFFTSNHFRDAQVMG
                     RSVLTRAPRLRIDPRARRRPLRFGYLGTLSTHRFINVSAQRVGDQLYLWGSGAYRADD
                     LRLAGVDLTVPGLAQGLRAGNAAPLLAAVRYWAGPQVETSDSVIPANAGISTPRPTVS
                     TSSTGGAGSTDSGQAWSTREADARPLFTPGAHGEVSVRWVSQVRRYLMLTMSGPDDTI
                     GPAVVLRWAANPWGPWSSRLRLFDWIDSGMSFGDPAHRFIKAMADGSDPVGDAIFRGQ
                     STATGAAYAPYFYDARVEGDALVLRYTLSTWNPYQVVLMQHRLELARLATEQAKGGSD
                     GPADQVR"
     gene            complement(3553..3843)
                     /locus_tag="PLF56_04835"
     CDS             complement(3553..3843)
                     /locus_tag="PLF56_04835"
                     /inference="COORDINATES: ab initio
                     prediction:GeneMarkS-2+"
                     /note="Derived by automated computational analysis using
                     gene prediction method: GeneMarkS-2+."
                     /codon_start=1
                     /transl_table=11
                     /product="hypothetical protein"
                     /protein_id="HOQ52921.1"
                     /translation="MNPPSAGPIGQRLVLIGLAIAALTFIGFGVQAAVQHGRPWWPSA
                     VVPLVTGASAGLIVWLNRRRVRGSVTDGVLRIGLPVLGLVLIGFALFLIYRT"
     gene            4112..5689
                     /gene="glpA"
                     /locus_tag="PLF56_04840"
     CDS             4112..5689
                     /gene="glpA"
                     /locus_tag="PLF56_04840"
                     /EC_number="1.1.5.3"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_016456920.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_component: GO:0009331 - glycerol-3-phosphate
                     dehydrogenase complex [Evidence IEA];
                     GO_function: GO:0004368 - glycerol-3-phosphate
                     dehydrogenase (quinone) activity [Evidence IEA];
                     GO_process: GO:0006071 - glycerol metabolic process
                     [Evidence IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="anaerobic glycerol-3-phosphate dehydrogenase
                     subunit GlpA"
                     /protein_id="HOQ52922.1"
                     /translation="MKTLSADVVVVGGGSTGAGVVRDVAMRGYTAILVDRADLAQGTT
                     GRYHGLLHSGGRYVVSDPHSATECAEENAIVTRINADAVETTGGYFVATEVDDPAWGD
                     DWAKGATDTKVPFEEVSVTEVLTAEPRVNPKISRAFKVLDGTVDGWQMVWGAANSAKH
                     YGAQVLTYHRVTGINRDGDRVSQVICTDEKTGEQVRIDTNFVLNCAAAWGGQIAAMAD
                     CHDVQVVPGAGIMIAMNHRLTNSVVNRLTYPADGDILVPVHTVCVIGTTDKKADDPDQ
                     LQIAWDEVQQMMDCGELLVPGFRKARAIHAWAGARPLVKDSRVGSGDTRHMSRGMSII
                     DHSERDGLKGLLTIAGGKLTTYRLMAEKVVDAMCTQLDDPRPCRTATEAVPGSETGKN
                     YVITHRLDEREHDRTEDQILCECELMSKGMFTRALADQPKGSFDDLRRQLRLGMGPCQ
                     GGFCSMRATGVALQSEHIDIERATGLLRLFLKNRWIGIWPILYGDQVRQTALDNWIFQ
                     GTLDVEHLPGPTHEEVV"
     gene            5689..>6812
                     /gene="glpB"
                     /locus_tag="PLF56_04845"
     CDS             5689..>6812
                     /gene="glpB"
                     /locus_tag="PLF56_04845"
                     /EC_number="1.1.5.3"
                     /inference="COORDINATES: protein motif:HMM:NF003724.0"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="glycerol-3-phosphate dehydrogenase subunit GlpB"
                     /protein_id="HOQ52923.1"
                     /translation="MTRVVVIGAGLAGLVASIRLADAGARVALLNKGTGGLQLGQGTI
                     DVLGYSPERVQRPLDELAGFADRHPGHPYGLLDAALVGRSVDYLKELLGPGLVTGDPA
                     VNLQLPTAVGAIRPTALASPSMTAGACVDGAEFVIVGLRRLKDFYPELVAANLSRTDL
                     PGGGRLRARPVVLDIPVREHEVDSSGLTYARALDEPDYRRRFAQAVKAVVGEGETVGL
                     PAVLGLDDHGAWAEIAELIGAPVFEIPLPPPSVPGMRQNRALLARAKAAGVRVVPGSR
                     VTGFRSSGGAVDSVQLATAGGDRDFVAEAFVLATGGFESGSLSADSYHNLHEAVFDLP
                     LRRPEGALVHGDYWGDPQPLFAVGVETGPDLLVHHPGGDAV"
BASE COUNT         1049 a         2333 c         2409 g         1021 t
ORIGIN      
        1 agcacgacga acaccagcac gaaggtggcc acggcctcgg tgaccaggtt ccacaccgtg
       61 tcgcgaacct cgggaccggt ggagaacacg ccgagcttga agccctcggg ggcgtcgccg
      121 tcgaactgct tcttgtaggc gagccaggcc agcacggcgc cgatgaaggc gcccaccatc
      181 tgcgcaccga tgtaggtgag gatcgaggcg aagttcaccg gaatgcccgg cacgaaatcg
      241 gtggcgccgg acgccacgat gcccagggtg acggccgggt tcagatgccc gcccgacttg
      301 tacgacacga ggacgccggc gaagaccgcc agcccccagc cgaagttgac catcaggaac
      361 ccggttccct cgcccttgtt cttcggcaga atgttgttgg caaccacacc ggcgccgagc
      421 agcagcagca tcgctgtgcc gacggtttcg gagaggaaga tggttgcaag agtcactgaa
      481 atcactccct tcaggcaccg gtcagccggt gccgtgcagt gtcgaggggt cctcccccga
      541 caacgaaaaa ctacgccgca ccagtgggtg cgacggggat ttgcggggtg gacggtgacg
      601 tgagccgcgc attctgtgcc cctgccaccg gacggtcacc cgtggccgtc gggccccagt
      661 cttgccagca cctgcacgga tgcgctagtt ctgtgcacga acgtgcaacg ggaggtcgaa
      721 tgaacgcgcg atacgacgac atgtaccagg cggcgtcccg ctactacatc cagggcgaga
      781 cgatggacgc catcgccaag cagttgggct tgtcccgctc gacggtcagc cgcctgctca
      841 aggacgcgcg cgccagcggt ctggtacgca tcacgctggc cgatcacacc ggctcccgct
      901 cgccgctcgc cggcgcactg gtgaaaacgt tcggggtgcg ggtgcacctg gtgacggtgc
      961 gcgacagcct ttccgactac cggcgcttcg atcaggtagc ccggttggcc ggacgcctgc
     1021 tctctgaggc ggtggccgac cagtgcacga tcggcatcgc gtggggggtg accgcgtccg
     1081 ccgtggtgcg ctacctgcag cccaagccgc tggtcggttc gaccgtggtg cagctcaacg
     1141 ggggcaccaa ccagtggggc tcgggcattc cctacgtggg tgagattctg cagagcgtct
     1201 gcgacgcgtt cagttcgcgg gtggtctact ttccggttcc cgcattcttc gactacgccg
     1261 aaaccaagag tgcgatgtgg cgcgagcgtt cggtgcagaa cgtggtcggg ttgcagcggc
     1321 gaacggacgt cgcggtgttc ggggtggggt gcctgagggc gcgggtgccg tcccacgtct
     1381 acagcgccgg ctatctggac gatgacgaca agcgtgcgct gagcgccgac ggcgtggtcg
     1441 gcgacgtggc gacggtgctg ttgcgcgagg acggttcgtg gaaggacatc gggttcaacg
     1501 aacgcgcctc ggggctcacg ccggccgaac tggcccgggt gccacgccgg ttctgcgtgg
     1561 tggccgatcc gtcacgtgcc gccgcggtgg tgggggcact tcgcgccggt accaccaccg
     1621 acctggtgat cgacaacgcc accgcgctgg ccgtactgga gcggctgtag tgccgcggcc
     1681 acgagccgtg atcgggccgg cgtcgttgct ggagtcggcg gatcgtccgg cctggtggac
     1741 gccgcccggc cggccgcacc ggctggaggc gctgatcgga gacccccagg ccgacggcac
     1801 gctgcgcatc gagcactggt tctgtggcca gcatcgtccg gacaacccgt gggtacgcgg
     1861 cgacgcgatc agcgatcggg cgctggcgcc ggcagcactg acccagcggg gacgtcccgg
     1921 cacggcggcc cacgaactgg tggcgcacat acccgaggcc gacggcctga tcgagtacgc
     1981 ctgggcggtg aaccagtcat cgccgatgcg gtggcgccgc gtgggtgccg ccccggctgt
     2041 tccggcgccg agacgtccgg cctgggacgg ccccgagcac gcggccgagt ggggtggcgg
     2101 caccgtgatc gccaccgcga ccgccgccac ccggctgcgg tcgggctggg tgcaggcgct
     2161 ggtgcaggtg gacgactcgc tctaccacct gcatcgccag cagcacggtc agcgggtgcg
     2221 ctgggtgcgg cacgcgtgcc tgcggttgcg ggatcacgac gctttcgagg tggcgcccga
     2281 gccgtcggtc aagctcgcgc aggtcaccgg cgaacgcgac tcgcagcccc gtccgggttc
     2341 gacgggggcg accctgtcgt cctcggagtc ggtcgccggg gtgcgtggta ccgatctggg
     2401 cgtgcgcgtc gaggcggacg ggcgctcgtt cctgctgttc ggcgacaccc actggacccg
     2461 gccctggctg ggcacccgcg actcgatcgc cgaggtgcgg gacgcctccg cggaacgtcc
     2521 ggacgtggtg ttccacggct cgcccctgtc gatcaggggc ggcggcacca ccctgcgcga
     2581 gtacgacgtg ccgctggacg ccttcagcct ggagggcaga cagtacgcgt tcttcacctc
     2641 caaccacttt cgcgacgcac aggtgatggg acgcagcgtg ctcacccggg caccacggct
     2701 gcggatcgac ccacgggcac gccggcgtcc gctgcggttc ggctacctgg gcactctctc
     2761 gacccaccgc ttcatcaacg tctcggcgca gcgggtcggc gatcagctgt acctgtgggg
     2821 cagcggcgcc taccgcgcgg acgacctgcg gctggccggt gtcgatctga ccgtccccgg
     2881 cctggcgcag ggcctgcggg ccggcaacgc cgcacccttg ctggccgcgg tccgctactg
     2941 ggcggggccg caggtcgaga cctccgactc cgtcatcccg gcgaacgccg ggatctccac
     3001 cccgcgcccc acggtctcga caagctcgac cggcggggca ggctcgaccg actccggcca
     3061 ggcctggtcc acccgtgagg cggacgcgcg tccgttgttc accccgggcg cccacggcga
     3121 ggtctcggtg cgctgggtgt cgcaggtgcg gcgctacctg atgctcacca tgtcgggccc
     3181 ggacgacacc atcgggcccg cggtggtgct gcggtgggcg gccaatccct gggggccgtg
     3241 gtcgtcccgg ctgcggctgt tcgactggat cgactcgggc atgagcttcg gcgaccccgc
     3301 ccaccgcttc atcaaggcca tggccgacgg cagtgatccg gtgggtgatg ccattttccg
     3361 tggccagtca acggccaccg gggccgcgta cgcgccctac ttctacgatg cccgggtcga
     3421 gggggacgcg ctggtgctgc gatacacgct gtccacctgg aacccctacc aggtcgtgct
     3481 gatgcagcat cgtctcgagc tggcccggtt ggccacggag caggcgaagg gcggctctga
     3541 cgggccggcg gatcaggttc ggtagatcag aaacagcgcg aacccgatca acaccagtcc
     3601 cagcaccggc aggccgatcc gcagcacgcc gtcggtgacg gacccccgca cccgccggcg
     3661 gttcaaccag acgatcaggc cggccgacgc gcccgtcacc agcggaacca ccgcgcttgg
     3721 ccaccagggc cggccatgct gcaccgcggc ctgcacgccg aagccgatga aggtgagtgc
     3781 agcgatagcc agaccgatga gcaccaaccg ctggccgatc ggcccggccg agggcgggtt
     3841 catgctcgca gcgtaacccg cgacgccccg tccgaacggc acctcttgtc gtgatcgatg
     3901 gtggcggtag agaatccggt tgtaatctca catcaaatgt gaaaaatgca tccagcgctg
     3961 atgcacagag gtcgatcggg ctcaccgtgg cttgcacatc cgctccccgt gcgacgtgtg
     4021 gccccgcagc ggcgctgatg ctgaatagcc tggtcagcaa caacccccag tgactggacg
     4081 tcgtcgtccg gcccaccgat cggaggtcaa ggtgaagaca ctctcagcag acgtcgtcgt
     4141 ggtgggtggt ggctcgaccg gcgccggcgt cgtgcgcgac gtcgcgatgc gcggctacac
     4201 ggcgatcctg gtcgaccggg ccgacctggc ccagggcacc accgggcgct accacggcct
     4261 gctgcattcc ggcggacgct acgtggtcag cgatccacac tcggccaccg aatgcgccga
     4321 ggagaacgcg atcgtcaccc gcatcaacgc cgatgccgtc gaaaccaccg gcgggtactt
     4381 cgtcgccacc gaggtggacg acccggcctg gggcgacgac tgggccaagg gcgcgaccga
     4441 cacgaaggtg cccttcgagg aggtgtcggt caccgaggtg ctcacggccg agccgcgggt
     4501 caacccgaag atcagccgtg ccttcaaggt actggacggc acggtcgatg gctggcagat
     4561 ggtgtggggc gcggccaact cggccaagca ctacggcgcg caggtcctca cctaccatcg
     4621 ggtgaccggc atcaaccgcg acggcgaccg ggtcagccag gtgatctgca ccgatgagaa
     4681 gaccggcgag caggtgcgca tcgacaccaa cttcgtgctg aactgcgcgg ccgcctgggg
     4741 cggtcagatc gcggcgatgg ccgactgcca cgacgtgcag gtggttccgg gcgccggcat
     4801 catgatcgcg atgaaccacc ggctgaccaa ctcggtggtc aaccgcctga cctaccccgc
     4861 tgacggcgac attctggtgc cggtgcacac ggtgtgcgtg atcggcacca ccgacaagaa
     4921 ggccgacgac cccgaccagc tgcagatcgc ctgggacgag gtgcagcaga tgatggactg
     4981 cggtgagttg ctggtgcccg gcttccgcaa ggcacgcgcg atccacgcct gggcgggcgc
     5041 ccgtccgctg gtgaaggact cgcgggtcgg ctccggtgac acccggcaca tgagccgtgg
     5101 catgtcgatc atcgaccact ccgagcgcga cgggctgaag ggcctgctca ccatcgccgg
     5161 cggcaagctg accacctacc ggctgatggc cgaaaaggtg gtggacgcca tgtgcaccca
     5221 gctggacgac ccccgtccgt gccgcaccgc caccgaagcc gtgccgggct ccgagacggg
     5281 caagaactac gtgatcacgc accggctcga cgagcgcgag cacgaccgca ccgaggacca
     5341 gatcctgtgc gagtgcgagc tgatgagcaa gggcatgttc acccgcgccc tcgccgatca
     5401 gcccaagggc agcttcgacg acctgcgccg ccagctgcgg ctcggcatgg gcccctgtca
     5461 gggcggcttc tgttcgatgc gggccaccgg cgtggcgctg cagagcgagc acatcgacat
     5521 cgagcgtgcg accggcctgc tgcggctgtt cctgaagaac cggtggatcg gcatctggcc
     5581 gatcctgtac ggcgaccagg tgagacaaac agcactcgac aactggatct tccagggaac
     5641 gctcgacgtc gagcaccttc ccggcccgac gcatgaggag gtcgtgtgat gacacgagtc
     5701 gtggtgatcg gcgccggtct ggccgggctg gtcgcgtcca ttcgactggc ggacgccggg
     5761 gcgcgcgtcg ccctgctgaa caagggcacc ggcggcctgc agctcggcca gggaacgatc
     5821 gacgtgctcg gctactcccc cgagcgggtg cagcgtccgc tggacgagct cgccggcttc
     5881 gccgatcgtc atcccggcca tccctacggc ctgctggacg cggcgctggt cggccgctcg
     5941 gtcgactacc tcaaggagct gctcgggccg ggcctggtga ccggcgaccc ggcggtcaat
     6001 ctgcagctgc cgaccgcggt gggcgcgatc cgtccgacgg cgctggcctc gcccagcatg
     6061 accgccggcg cctgtgtgga cggggccgag ttcgtcatcg tcgggttgcg ccggctcaag
     6121 gacttctacc ccgaactggt ggccgcgaac ctctcgcgca ccgacctgcc cggcggcgga
     6181 cggctgcgtg cccggcccgt ggtgctcgac attcccgtgc gcgagcacga ggtcgactcc
     6241 agtgggctca cctacgcccg ggcgctggac gagccggact accggcgtcg cttcgcccag
     6301 gccgtcaagg cggtcgtcgg tgagggcgag acggtcggcc tgcccgccgt gctgggcctg
     6361 gacgaccacg gggcctgggc cgagatcgcc gagttgatcg gggcaccggt gttcgagatt
     6421 ccgctgccac cgccgtccgt gccgggcatg cggcagaacc gtgcgctgct cgcgcgggcc
     6481 aaggcggcgg gcgtccgggt ggtgccgggt agccgggtga ccggttttcg tagttcgggc
     6541 ggcgcggtcg acagcgtgca actcgccacg gccggcggtg accgcgactt cgtggcagag
     6601 gctttcgtgc ttgccacggg tggtttcgag tcgggctcac tgagcgctga ctcgtaccac
     6661 aacctgcacg aggcggtctt cgacctgccg ttgcgccgac cggagggagc gctggtgcat
     6721 ggcgactact ggggcgaccc gcagccgctg ttcgccgtcg gtgtcgagac cggccccgac
     6781 ctgctggtcc accaccccgg cggggacgcc gt
//
LOCUS       DAOSUG010000103         6767 bp    DNA     linear   ENV 10-JUN-2024
DEFINITION  MAG TPA_asm: Micropruina sp. isolate AMR_MDS_2195
            NODE_5440_length_6767_cov_4.727503, whole genome shotgun sequence.
ACCESSION   DAOSUG010000103 DAOSUG010000000
VERSION     DAOSUG010000103.1
DBLINK      BioProject: PRJNA925477
            BioSample: SAMN32864262
            Sequence Read Archive: SRR4236673
KEYWORDS    WGS; ENV; Metagenome Assembled Genome; MAG; Third Party Data; TPA;
            TPA:assembly.
SOURCE      Micropruina sp. (wastewater metagenome)
  ORGANISM  Micropruina sp.
            Bacteria; Bacillati; Actinomycetota; Actinomycetes;
            Propionibacteriales; Nocardioidaceae; Micropruina.
REFERENCE   1  (bases 1 to 6767)
  AUTHORS   Abdulkadir,N., Saraiva,J.P., Zhang,J., Stolte,S., Gillor,O.,
            Harms,H. and Rocha,U.
  TITLE     Genome-centric analyses of 165 metagenomes show that mobile genetic
            elements are crucial for the transmission of antimicrobial
            resistance genes to pathogens in activated sludge and wastewater
  JOURNAL   Microbiol Spectr 12 (3), e0291823 (2024)
   PUBMED   38289113
REFERENCE   2  (bases 1 to 6767)
  AUTHORS   Abdulkadir,N.
  TITLE     Direct Submission
  JOURNAL   Submitted (24-MAR-2023) Environmental Microbiology, Helmholtz
            Center for Environmental Research -UFZ, Permoserstr 15, Leipzig
            04318, Germany
COMMENT     The annotation was added by the NCBI Prokaryotic Genome Annotation
            Pipeline (PGAP). Information about PGAP can be found here:
            https://www.ncbi.nlm.nih.gov/genome/annotation_prok/
            
            ##Genome-Assembly-Data-START##
            Assembly Method        :: SPAdes v. 3.11.1
            Genome Representation  :: Full
            Expected Final Version :: Yes
            Genome Coverage        :: Not Applicable
            Sequencing Technology  :: Illumina MiSeq
            ##Genome-Assembly-Data-END##
            
            ##Genome-Annotation-Data-START##
            Annotation Provider               :: NCBI
            Annotation Date                   :: 05/04/2023 04:57:10
            Annotation Pipeline               :: NCBI Prokaryotic Genome
                                                 Annotation Pipeline (PGAP)
            Annotation Method                 :: Best-placed reference protein
                                                 set; GeneMarkS-2+
            Annotation Software revision      :: 6.5
            Features Annotated                :: Gene; CDS; rRNA; tRNA; ncRNA
            Genes (total)                     :: 2,776
            CDSs (total)                      :: 2,750
            Genes (coding)                    :: 2,714
            CDSs (with protein)               :: 2,714
            Genes (RNA)                       :: 26
            tRNAs                             :: 24
            ncRNAs                            :: 2
            Pseudo Genes (total)              :: 36
            CDSs (without protein)            :: 36
            Pseudo Genes (ambiguous residues) :: 0 of 36
            Pseudo Genes (frameshifted)       :: 2 of 36
            Pseudo Genes (incomplete)         :: 32 of 36
            Pseudo Genes (internal stop)      :: 2 of 36
            ##Genome-Annotation-Data-END##
FEATURES             Location/Qualifiers
     source          1..6767
                     /organism="Micropruina sp."
                     /mol_type="genomic DNA"
                     /submitter_seqid="NODE_5440_length_6767_cov_4.727503"
                     /isolate="AMR_MDS_2195"
                     /isolation_source="Activated sludge of municipal
                     wastewater treatment plants"
                     /db_xref="taxon:2737536"
                     /environmental_sample
                     /geo_loc_name="Canada"
                     /lat_lon="49.25 N 123.1 W"
                     /collection_date="2015-06-16"
                     /metagenome_source="wastewater metagenome"
                     /note="metagenomic"
     gene            complement(<1..304)
                     /locus_tag="PLF56_04850"
     CDS             complement(<1..304)
                     /locus_tag="PLF56_04850"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_003419511.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="Clp protease N-terminal domain-containing
                     protein"
                     /protein_id="HOQ52924.1"
                     /translation="MFDRFTDRARRVIVLAQDEARMLNHNYIGTEHILLGLIHEGEGV
                     AAKALEALGISLEAVREQVEEIIGQGQQAPTGHIPFTPRAKKVLELSLREALQLGHN"
     gene            complement(482..805)
                     /locus_tag="PLF56_04855"
     CDS             complement(482..805)
                     /locus_tag="PLF56_04855"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_016456721.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="Lsr2 family protein"
                     /protein_id="HOQ52925.1"
                     /translation="MAQRIVYRLEDDLDNSEAAETIVFGIDSVSYEIDLSESNAQKLR
                     DLLAPYVASGRRVGGRRKAAGRRRAGGSSAGEIRVWAAENGYNVSARGRVPAEVREAY
                     DRAHG"
     gene            complement(1040..1576)
                     /locus_tag="PLF56_04860"
     CDS             complement(1040..1576)
                     /locus_tag="PLF56_04860"
                     /inference="COORDINATES: ab initio
                     prediction:GeneMarkS-2+"
                     /note="Derived by automated computational analysis using
                     gene prediction method: GeneMarkS-2+."
                     /codon_start=1
                     /transl_table=11
                     /product="hypothetical protein"
                     /protein_id="HOQ52926.1"
                     /translation="MKPRTGRLSRPPAVRILASTMLALVAGVASPAVVFGALPVRGVA
                     LAALLAGALLVCTLAWRERRLIERARLAAELGQAMQHAEQLHAERARQSAVLRVLSSR
                     LGELRDERDQARALSSQLQQQLSTLRGNHEALRVELELQAVLAAGANGPAPHRSQPAS
                     AAVGEPAGISVVLVPQSA"
     gene            complement(1586..2098)
                     /locus_tag="PLF56_04865"
     CDS             complement(1586..2098)
                     /locus_tag="PLF56_04865"
                     /inference="COORDINATES: protein motif:HMM:NF022819.2"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="DUF3180 domain-containing protein"
                     /protein_id="HOQ52927.1"
                     /translation="MPQPGPTVHPTGRTALAVAALIGAGLTWIALSVAESFAWPLPTV
                     PVLAAVVVAVLALATWLTARWTHRTVQVRRRTIEPARAVGLVLAGKAALLGGTALAAG
                     YLVLVLRQLPNVDATLPRERVLVASVVAVLSVGLAGAGWALERACQVPPDDDSDDAPK
                     GQPGGAPSPG"
     gene            complement(2098..2670)
                     /gene="folK"
                     /locus_tag="PLF56_04870"
     CDS             complement(2098..2670)
                     /gene="folK"
                     /locus_tag="PLF56_04870"
                     /EC_number="2.7.6.3"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_007078951.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_function: GO:0003848 -
                     2-amino-4-hydroxy-6-hydroxymethyldihydropteridine
                     diphosphokinase activity [Evidence IEA];
                     GO_process: GO:0009396 - folic acid-containing compound
                     biosynthetic process [Evidence IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="2-amino-4-hydroxy-6-
                     hydroxymethyldihydropteridine diphosphokinase"
                     /protein_id="HOQ52928.1"
                     /translation="MSEPDFYPNAHLDFDVDTLVGMVPIRPVVFSLGSNLGDRLAYLQ
                     AAVDALRATPNLSVVDVSPVYETDPVGPVLDQPKFLNAVVRADTALASLVLLERAQAI
                     ENAYRRTRELPGGPRTLDVDVIVVGERIKNTPVLTLPHPRARERAFVLVPWLDIDPAA
                     TLPEQGLVADLVATLDRSGIRLRPDLVIEL"
     gene            complement(2667..3038)
                     /gene="folB"
                     /locus_tag="PLF56_04875"
     CDS             complement(2667..3038)
                     /gene="folB"
                     /locus_tag="PLF56_04875"
                     /EC_number="4.1.2.25"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_007078952.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_function: GO:0004150 - dihydroneopterin aldolase
                     activity [Evidence IEA];
                     GO_process: GO:0006760 - folic acid-containing compound
                     metabolic process [Evidence IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="dihydroneopterin aldolase"
                     /protein_id="HOQ52929.1"
                     /translation="MAATVPDRVRLTGLRFSAIHGVHDFERTQPQAFEVDLDCALAPR
                     PHSDDLATTVDYAQLSRTVESAVTGDPVNLIESLAERIAAACLQHPRIDSVEVTVHKP
                     DAAMPVDLRDVAVTIRRSRQA"
     gene            complement(3060..3857)
                     /gene="folP"
                     /locus_tag="PLF56_04880"
     CDS             complement(3060..3857)
                     /gene="folP"
                     /locus_tag="PLF56_04880"
                     /EC_number="2.5.1.15"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_014845544.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_function: GO:0004156 - dihydropteroate synthase
                     activity [Evidence IEA];
                     GO_process: GO:0009396 - folic acid-containing compound
                     biosynthetic process [Evidence IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="dihydropteroate synthase"
                     /protein_id="HOQ52930.1"
                     /translation="MGIVNVTPDSFSDGGAYLDAEAAIAHGRDLLADGADIVDVGGES
                     TRPGAVRVSVETELNRVVPVISAISAAGGIVSVDTMRAEVAQAAVGAGATIVNDVSGG
                     LGDPAMLGTVARLGVGYIAMHWRGQSDRMQSLAVYDDVVTDVCAELAARRDAALAAGI
                     APRNLVLDPGLGFAKTAAHNWSLLAHLDALQGLGHPLLVGASRKRFLGELLSGRPPQE
                     RDDATLALTTLLAQQGVWAVRTHAARAHRDAIAVVRRMGSEAVRGVQ"
     gene            complement(3946..4863)
                     /locus_tag="PLF56_04885"
     CDS             complement(3946..4863)
                     /locus_tag="PLF56_04885"
                     /inference="COORDINATES: protein motif:HMM:NF016370.2"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="DUF559 domain-containing protein"
                     /protein_id="HOQ52931.1"
                     /translation="MSRAALTSSGVTDAMIRSALVAGRLHRIRQGVYIEAAMWPPEPR
                     EQHLLRAVAELTLHPTGVLSHQSAATAWGLPHPGFSQWYECGVSITLPRAEAARSRVK
                     PARHHVAPLPAAQVTRDAGGRPVTSLARTAVDLAAGRPLPDGLVILDGAARRLCAAMV
                     AQPRRSDYANPRLRTAVRQLFEQSASSRCAATLLLAIDLADPRRESAPESLSAGHFAL
                     AGLPEPLCQPRFETPIGQLYPDFYWPEQMLIGECDGAVKYADAQGYVREKEREQALRD
                     QGYAMVRWQAKEIMLRPEVVVERVARCLN"
     gene            complement(5198..5761)
                     /gene="folE"
                     /locus_tag="PLF56_04890"
     CDS             complement(5198..5761)
                     /gene="folE"
                     /locus_tag="PLF56_04890"
                     /EC_number="3.5.4.16"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_007078955.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_function: GO:0003934 - GTP cyclohydrolase I activity
                     [Evidence IEA];
                     GO_process: GO:0042559 - pteridine-containing compound
                     biosynthetic process [Evidence IEA];
                     GO_process: GO:0046654 - tetrahydrofolate biosynthetic
                     process [Evidence IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="GTP cyclohydrolase I FolE"
                     /protein_id="HOQ52932.1"
                     /translation="MAVDQPRVAAAVRELLVAIGDDPDREGLRDTPARVARAYAEMFV
                     GIDQSADEVLATTFDIAHDELVLVKDIEVWSICEHHLVPFTGVAHVGYIPAVEGRITG
                     LSKLARLVDVFAKRPQVQERLTTQVADALVEHLRPRGVIVVIECEHLCMTMRGVRKPG
                     SKTVTSAVRGALRNPATRAEAMSLIIG"
     gene            complement(5763..>6767)
                     /locus_tag="PLF56_04895"
     CDS             complement(5763..>6767)
                     /locus_tag="PLF56_04895"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_013865685.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="cell division protein FtsH"
                     /protein_id="HOQ52933.1"
                     /translation="AIDRVIAGPQKRSRIMNERELLITAYHEGGHALVAAAMPGTDPV
                     QKVTILPRGRALGYTMVMPDSDKYSKTRGELLDQLAYMLGGRAAEELVFHDPTTGAAN
                     DIEKATNTARAMVTKYGMTERLGAVKLGNDSDEPFLGMQAGMGSGHRDYSDAVAAAID
                     DEVAALINTAHQEAFDCLVLNREVLDELVRQLFEKETLDKEQVATIFEKLQRHPKRPA
                     WTGSQTRVPSEVPPVDPPPPAPKPDPTYGPPLLGPNGNIQVPVPTGPGAGGPMPPYDV
                     GPSGPQAQPPSGPPYGQQGTPPWQTPYGQGQYGPGQPGPDGATPPQQPDGGWGPSQGR
                     "
BASE COUNT         1041 a         2350 c         2361 g         1015 t
ORIGIN      
        1 agttgtggcc gagctgcagc gcttcgcgca gcgacagctc gagcaccttc ttggcccgcg
       61 gcgtgaacgg gatgtgcccg gtcggcgcct gctggccctg gccgatgatc tcttcgacct
      121 gctcgcggac ggcctcgagc gaaatgccca gcgcctccaa agccttggcg gcgacgcctt
      181 caccctcgtg aatcagcccg agcagaatgt gctcggtgcc gatgtagttg tggttcagca
      241 tgcgggcctc gtcctgcgcc aggacgatga cccgtcgtgc acggtcggtg aaccggtcga
      301 acatattccg cactcctcac tccggcctgc gccggggggc gctccggcag ggtcggccga
      361 cccggcccgg gcgccatcag tctattcaac tcacgcgagc agagttctgt tccggcttcg
      421 cccacggcga acggccccgg aatgactccg gggccgttgc gctgcgtcag gtgtggcttg
      481 gtcagccgtg cgcccggtcg tacgcctcgc gcacctcggc cggaacccgt ccgcgggccg
      541 agacgttgta gccgttctcg gcagcccaga cccggatctc gccggccgaa ctgccgcctg
      601 cgcgccgccg cccggcggcc ttgcggcgcc cgccgacccg ccgtccggac gccacgtagg
      661 gcgccagcag gtcgcgcagt ttctgggcgt tcgactcgga cagatcgatc tcatagctga
      721 ccgaatcgat accgaagaca atggtctcgg ccgcttcgct gttgtccaga tcgtcttcga
      781 gacgatagac gatgcgttgc gccatatggt tacctcccac gttgatggat tgtgccactt
      841 cgagctgttc ccccgttcag cctcagtgca agatcaccct acatgcaaac aaatatcatg
      901 caaagtgacc gggcccgcgc acgcaatgca aaatctttcc ttcagctgtg catcgcctga
      961 gaccgtggac gggcgccgga cgcgccgacg ccaatgcatg gggcgaaatc gaccattgcg
     1021 gccccgcaca aatgcgcgat caggcggatt gcggcacaag cacgaccgaa atgcccgctg
     1081 gttcgcccac agctgcggac gcgggctgcg atcggtgagg agccggccca ttcgcgcccg
     1141 ccgccaacac cgcctgcaac tcgagttcga cccggagtgc ctcgtgattg ccgcgcaagg
     1201 tggacagttg ctgctgcagt tgagacgaaa gcgcgcgggc ctgatcgcgc tcgtcacgca
     1261 actcgcccag ccgagaactc agcacccgca gcacggccga ctgccgagcg cgttcggcgt
     1321 gcaactgctc ggcgtgctgc atggcctgac cgagctcggc ggccaggcgg gcgcgctcga
     1381 tgaggcgccg ctcgcgccag gccaaggtgc acaccagcag ggcgccggcc agcagggcgg
     1441 ccagcgccac cccgcgaacc ggaagcgcac cgaacaccac cgccggcgag gcaacgccgg
     1501 cgaccagcgc gagcatcgtg gacgcgagaa tgcggacggc cggaggccgg ctcagtcgac
     1561 ccgttcgagg cttcacgagc gcagcctaac cggggctggg cgcgcccccc ggctggccct
     1621 tgggcgcgtc gtccgaatcg tcgtcgggcg gcacctggca ggcccgttcg agtgcccaac
     1681 cggcgccagc gaggcccacg ctgagcaccg cgaccaccga ggccaccagc actcgctcac
     1741 gcggcagtgt ggcgtccacg ttcggcaact ggcgcagcac cagcaccagg tagcccgcgg
     1801 ccagcgccgt gccaccgagc agcgccgcct tgccggccag caccagaccc accgcccggg
     1861 cgggttcgat ggtcctgcgg cggacctgca ccgtacggtg cgtccagcgg gcggtcagcc
     1921 aggtggccag cgcgagcact gccaccacga cggcggccag caccggaaca gtcggcagtg
     1981 gccaggcgaa actctcggcc acggacagcg cgatccaggt caggcccgcg ccgatcagtg
     2041 cggcgaccgc caacgccgtg cgtccggtcg ggtgcaccgt ggggccgggt tgtggcatca
     2101 cagctcgatg acgagatcag gacgcagccg gatgcccgac cggtccagtg tggccacgag
     2161 atccgccacc agaccctgtt cgggaagtgt ggcggcgggg tcgatgtcga gccatggcac
     2221 cagcacgaac gcccgctcac gcgcacgcgg atgcgggagg gtgagcacgg gggtgttctt
     2281 gatgcgctca cccaccacga tcacgtccac atcgagggta cgcgggccgc cgggcagctc
     2341 tcgggtgcgg cgataggcgt tctcgatcgc ctgggcccgt tcgagcagca ccagggacgc
     2401 cagcgcggtg tcggcgcgca ccacggcatt gagaaacttc ggctggtcga gcaccggacc
     2461 gaccgggtcg gtctcataga ccggcgacac gtcgacgacg ctgaggttgg gggtggcgcg
     2521 cagtgcgtcc acagcggcct gcagataggc aagccggtcg ccgaggttgg accccagtga
     2581 gaacaccacc ggccggatcg gcaccatgcc gaccagggtg tcgacgtcga aatccaggtg
     2641 tgcgttcggg tagaagtcgg gctcgctcat gcctggcgac tcctgcggat ggtcacggcc
     2701 acatcgcgca ggtcgaccgg catggcggcg tccggcttgt ggacggtcac ctcgacggag
     2761 tcgatccgcg ggtgctgcag gcaagcggcc gcgatgcgtt cggcgagcga ctcgatcagg
     2821 ttgacgggat cacccgtgac cgcggattcg acggtgcgcg acaactgtgc gtagtcgacg
     2881 gtggtggcca ggtcatccga gtggggacgg ggggccaaag cacagtcgag gtcgacctcg
     2941 aacgcctgtg gctgggtgcg ctcgaagtca tgaacgccgt gaattgcgct gaaccgcaga
     3001 ccggtcagcc gcacccgatc gggaacggtt gctgccatgc actcacctcc gcaccgaccc
     3061 tactgcacgc cgcggacggc ctctgagccc atccgccgga ccaccgcgat ggcgtcgcga
     3121 tgggcccggg cggcgtgcgt acgcaccgcc cacacgccct gttgggcgag caacgtggtg
     3181 agggcgaggg tggcgtcgtc gcgctcctga ggcggacgtc cgctcagcaa ctcaccgagg
     3241 aaccgcttgc gcgaggcgcc caccagcagc gggtgcccga gcccctgcag agcgtccagg
     3301 tgagccagca ggctccagtt gtgggcggcg gtcttggcga agccgagtcc ggggtcgagc
     3361 accaggttgc ggggtgcaat gccggccgcc agggcggcat cacgtcgggc ggccagttcg
     3421 gcgcacacgt cggtgaccac gtcgtcatag accgcgaggc tctgcatgcg gtcggactgg
     3481 ccgcgccagt gcatggcgat atagccgacc ccgagccggg cgacggtgcc cagcatggcc
     3541 gggtcgccca ggccgccgga gacgtcgttg acgatcgtcg cgccggcgcc gaccgcggcc
     3601 tgcgccacct cggcgcgcat ggtgtcgacg ctgacgattc ctccggccgc gctgatcgcc
     3661 gagatcaccg gcacgacccg attcagctct gtctcgacgc tcacccgcac cgcgccgggc
     3721 cgggtcgatt cgccacccac gtcgacgatg tcggcaccat cggcaagcag gtcgcgtccg
     3781 tgggcgatgg cagcttcggc atcgaggtac gctccgccgt ccgagaacga gtcgggggtg
     3841 acattgacga tgcccatcac cagggtgcgc ccctgatcga aaagctgccg cacgcaggca
     3901 gcctagtggc agggcagaga gagtgcgccc ggcagccgtc acggctcagt tcagacagcg
     3961 cgcgacccgc tcgaccacca cctcggggcg cagcatgatc tctttggcct gccaacggac
     4021 catggcgtac ccctggtcgc gcagcgcctg ctcgcgctcc ttctcgcgga cgtacccctg
     4081 cgcgtcggcg tacttcaccg cgccgtcgca ctcgccgatc agcatctgct cgggccaata
     4141 gaagtcgggg tacagctgcc cgataggtgt ctcgaacctc ggttggcaca gcggctccgg
     4201 cagaccggcg agggcgaagt ggccggccga gagcgactcg ggcgccgact cgcgccgcgg
     4261 gtcggctagg tcgatcgcca gtagcagcgt cgcggcgcat cggcttgatg ccgactgttc
     4321 gaacagctgc cgcaccgcgg tgcgcagtcg cgggttggcg tagtccgatc gccgcggctg
     4381 agccaccatc gcggcgcaga gccgccgcgc ggcaccgtcg agaatcacca ggccgtcggg
     4441 cagcggccgc ccggcagcca agtcgacggc ggtgcgggcg agtgaagtga ccggacgccc
     4501 gcccgcgtca cgagtgacct gcgcggcggg caagggtgcg acgtggtgtc gagccggctt
     4561 cacccgggac cgtgcggctt cggcccgggg cagggtgatg ctgacgccac actcgtacca
     4621 ctggctgaag ccggggtgcg gcaatcccca ggcggtggcc gccgattggt ggctgagcac
     4681 cccggtcggg tgcagggtca gctctgcaac ggcccgcagc agatgctgct cgcgcggctc
     4741 aggtggccac atcgcggctt cgatgtagac gccctgcctg atccgatgca gtcgcccggc
     4801 caccaatgct gagcggatca tggcgtcggt cactcccgag gaggtcaggg cagctcggct
     4861 catcggctgc ttcggcaaca ggcacgggcg aggtgttcgc atccccacag gttgccggtc
     4921 tccaccggcg gaaagccgcc ccgagggatc gcagcggaga atctgtggat aaccttccct
     4981 cgactcggag tcagcacgac ctggcggggg gcggccatgc cctcatcgct tgcgcggccc
     5041 agtttctggt ggttatggcg cagactggcg ctctactggg acccaccgcc ttcaggcgcc
     5101 actatttggt tgcaatagat accccccagg ggtattggta tcaaccatac agtggcgtcg
     5161 ccggaacccg accgaccata accacggaaa gtggcgctca gccgatgatc aggctcatcg
     5221 cctcggccct ggtggccggg ttgcgcaagg caccccggac ggccgaggtg acggttttcg
     5281 accccggctt gcgcacgcct cgcatcgtca tgcacaaatg ctcgcactcg atcaccacaa
     5341 tgacccccct gggcctgagg tgctcgacca gggcgtccgc gacctgcgtg gtgagccgct
     5401 cctgcacctg ggggcgcttc gcgaacacat ccaccaggcg ggcgagctta ctcaggcccg
     5461 tgatgcggcc ctcgacggcc gggatgtacc ccacgtgcgc caccccggtg aacggcacca
     5521 ggtgatgctc acagatgctc cacacctcga tgtccttcac cagcaccagc tcgtcgtggg
     5581 cgatgtcgaa ggtggtggcc aacacctcgt ccgcgctctg gtcgatgccg acgaacatct
     5641 ctgcgtaggc ccgggccacg cgggccgggg tgtcgcgcag accttcgcgg tcggggtcgt
     5701 cgccgatcgc gaccagcagt tccctgaccg cggcggcgac ccgcggctgg tccaccgcca
     5761 cctcagcgcc cctgcgaggg cccccagccg ccgtccggct gctggggagg cgtggcaccg
     5821 tccgggccgg gctgtccggg gccgtactgc ccctgaccgt agggggtctg ccacggcggc
     5881 gtgccctgct ggccataggg cgggccggag ggcggctgcg cctgcgggcc ggacggaccc
     5941 acgtcgtagg gcggcatggg gccgccggcg ccggggccgg tgggcaccgg gacctggatg
     6001 ttgccgttgg gaccgagcag cggcggccca taggtcggat ccggcttggg tgcgggcggt
     6061 ggcgggtcga ccggcggaac ctcggagggc acccgggtct gcgatccggt ccaggccgga
     6121 cgcttcggat gccgctgcag cttctcgaag atcgtcgcga cctgctcctt gtcgagggtc
     6181 tccttctcga acagttggcg caccagctcg tccagcacct cgcggttcag caccaggcag
     6241 tcgaacgcct cctggtgcgc ggtgttgatc agggcggcca cctcgtcgtc gatggcggcg
     6301 gcgaccgcgt cggagtagtc gcggtgaccg ctgcccatgc cggcctgcat gcccaggaac
     6361 ggctcgtcgg agtcgttgcc cagcttgacg gcacccagcc gctcggtcat gccgtacttg
     6421 gtgaccatgg cccgggcggt gttggtggcc ttctcgatgt cgttggccgc gccggtggtg
     6481 gggtcgtgga agacgagttc ctcggcggcc cggccgccca gcatgtaggc cagctggtcg
     6541 agcagttcgc cgcgggtctt ggagtacttg tccgagtcgg gcatcaccat cgtgtagccg
     6601 agcgcgcggc cgcggggcag gatggtcacc ttctgcaccg ggtcggtacc gggcatcgcc
     6661 gcagcgacca gggcgtggcc gccctcgtgg taggcggtga tcagcagttc gcgctcgttc
     6721 atgatccggg agcgcttctg cggcccggcg atcacccggt cgatggc
//
LOCUS       DAOSUG010000104         6757 bp    DNA     linear   ENV 10-JUN-2024
DEFINITION  MAG TPA_asm: Micropruina sp. isolate AMR_MDS_2195
            NODE_5445_length_6757_cov_4.415846, whole genome shotgun sequence.
ACCESSION   DAOSUG010000104 DAOSUG010000000
VERSION     DAOSUG010000104.1
DBLINK      BioProject: PRJNA925477
            BioSample: SAMN32864262
            Sequence Read Archive: SRR4236673
KEYWORDS    WGS; ENV; Metagenome Assembled Genome; MAG; Third Party Data; TPA;
            TPA:assembly.
SOURCE      Micropruina sp. (wastewater metagenome)
  ORGANISM  Micropruina sp.
            Bacteria; Bacillati; Actinomycetota; Actinomycetes;
            Propionibacteriales; Nocardioidaceae; Micropruina.
REFERENCE   1  (bases 1 to 6757)
  AUTHORS   Abdulkadir,N., Saraiva,J.P., Zhang,J., Stolte,S., Gillor,O.,
            Harms,H. and Rocha,U.
  TITLE     Genome-centric analyses of 165 metagenomes show that mobile genetic
            elements are crucial for the transmission of antimicrobial
            resistance genes to pathogens in activated sludge and wastewater
  JOURNAL   Microbiol Spectr 12 (3), e0291823 (2024)
   PUBMED   38289113
REFERENCE   2  (bases 1 to 6757)
  AUTHORS   Abdulkadir,N.
  TITLE     Direct Submission
  JOURNAL   Submitted (24-MAR-2023) Environmental Microbiology, Helmholtz
            Center for Environmental Research -UFZ, Permoserstr 15, Leipzig
            04318, Germany
COMMENT     The annotation was added by the NCBI Prokaryotic Genome Annotation
            Pipeline (PGAP). Information about PGAP can be found here:
            https://www.ncbi.nlm.nih.gov/genome/annotation_prok/
            
            ##Genome-Assembly-Data-START##
            Assembly Method        :: SPAdes v. 3.11.1
            Genome Representation  :: Full
            Expected Final Version :: Yes
            Genome Coverage        :: Not Applicable
            Sequencing Technology  :: Illumina MiSeq
            ##Genome-Assembly-Data-END##
            
            ##Genome-Annotation-Data-START##
            Annotation Provider               :: NCBI
            Annotation Date                   :: 05/04/2023 04:57:10
            Annotation Pipeline               :: NCBI Prokaryotic Genome
                                                 Annotation Pipeline (PGAP)
            Annotation Method                 :: Best-placed reference protein
                                                 set; GeneMarkS-2+
            Annotation Software revision      :: 6.5
            Features Annotated                :: Gene; CDS; rRNA; tRNA; ncRNA
            Genes (total)                     :: 2,776
            CDSs (total)                      :: 2,750
            Genes (coding)                    :: 2,714
            CDSs (with protein)               :: 2,714
            Genes (RNA)                       :: 26
            tRNAs                             :: 24
            ncRNAs                            :: 2
            Pseudo Genes (total)              :: 36
            CDSs (without protein)            :: 36
            Pseudo Genes (ambiguous residues) :: 0 of 36
            Pseudo Genes (frameshifted)       :: 2 of 36
            Pseudo Genes (incomplete)         :: 32 of 36
            Pseudo Genes (internal stop)      :: 2 of 36
            ##Genome-Annotation-Data-END##
FEATURES             Location/Qualifiers
     source          1..6757
                     /organism="Micropruina sp."
                     /mol_type="genomic DNA"
                     /submitter_seqid="NODE_5445_length_6757_cov_4.415846"
                     /isolate="AMR_MDS_2195"
                     /isolation_source="Activated sludge of municipal
                     wastewater treatment plants"
                     /db_xref="taxon:2737536"
                     /environmental_sample
                     /geo_loc_name="Canada"
                     /lat_lon="49.25 N 123.1 W"
                     /collection_date="2015-06-16"
                     /metagenome_source="wastewater metagenome"
                     /note="metagenomic"
     gene            181..909
                     /locus_tag="PLF56_04900"
     CDS             181..909
                     /locus_tag="PLF56_04900"
                     /inference="COORDINATES: ab initio
                     prediction:GeneMarkS-2+"
                     /note="Derived by automated computational analysis using
                     gene prediction method: GeneMarkS-2+."
                     /codon_start=1
                     /transl_table=11
                     /product="hypothetical protein"
                     /protein_id="HOQ52934.1"
                     /translation="MSEWSQEQRHVSRAYDDWCLVEADIRAFLNLTVQWVTSAHDEAW
                     ITAQAVADQVFDPDKHDTDLSVGFYEDKVNQLWRQNHLWMVSAAALKDAVTAFEVYLE
                     IGALEAIRRHGFGFRTGRLESPSWSDLKKVHEFLGTTIDPEPVQYVRSLRHLLTHQRG
                     ELRTPESREQFAHESEGDRSYRREVTLDKQRVTQFMNDLATTVADCDRQIWGMAWGSL
                     HVPDLSPLSGRRGPLAAISKRAAK"
     gene            954..1886
                     /locus_tag="PLF56_04905"
     CDS             954..1886
                     /locus_tag="PLF56_04905"
                     /inference="COORDINATES: protein motif:HMM:NF024930.2"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="chromosome condensation regulator"
                     /protein_id="HOQ52935.1"
                     /translation="MNWLATLKRPTLPRAVTHDERQTGDMLTPFPDGRGPIAAGCRHS
                     IGRRIDGTVLTAGNGAAGECSVAGWSDVVAVAVGNVHAFSNTGKSHTLGLRADGTVLA
                     TGWNTHGQCNVGDWANVRAIAAGWRFSLGVTADGHVVATGRAAEGQIAVDEWEQVRSV
                     ACGDWHSLGLQAAGRVLAAGNNRAGQLNVHAWQDVVQVAAGYQHSLGLTADGRVIAAG
                     AKASGVREVTAWDSIAQVAAGSHHSVGLARTGTVAAAGSNAYGQCNTQEWRDIVAVAA
                     GAQHTLGLRADGTVLATGANDHGQCDVHGWADLA"
     gene            complement(2286..3182)
                     /locus_tag="PLF56_04910"
     CDS             complement(2286..3182)
                     /locus_tag="PLF56_04910"
                     /inference="COORDINATES: protein motif:HMM:NF021057.2"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="TIGR02391 family protein"
                     /protein_id="HOQ52936.1"
                     /translation="MTDKTPAYLRRTIESLERFQESFEAFMELHEEGSMSGPGLGYLP
                     TCWPKEGADANEAAKRTDAVARRAGECSSVAELTGAHMVVQGVGVVDPFSNWDTMLRP
                     KAFLYPSELRRIIARSLGVLSTMMAEAEEAEKSVALPVLGPASLHPTVWNAAAPFWTM
                     HQLRAAVQAAADAVVSAAKGLGVRNDIADTSIWQQLFSVNEPAPGRPRLRWPGDPEDR
                     DVKTMQDGLRQFAPGVQMTVRNPATHRQGVLTEQEAFELLATLSLLSRFMERCEVRSA
                     ESGEPKTDIGPQTNPARSPLPT"
     gene            3379..3756
                     /locus_tag="PLF56_04915"
                     /pseudo
     CDS             3379..3756
                     /locus_tag="PLF56_04915"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_015748734.1"
                     /note="incomplete; partial in the middle of a contig;
                     missing N-terminus; Derived by automated computational
                     analysis using gene prediction method: Protein Homology.
                     GO_function: GO:0003677 - DNA binding [Evidence IEA];
                     GO_function: GO:0004803 - transposase activity [Evidence
                     IEA];
                     GO_process: GO:0006313 - transposition, DNA-mediated
                     [Evidence IEA]"
                     /pseudo
                     /codon_start=1
                     /transl_table=11
                     /product="transposase"
     gene            complement(4490..5275)
                     /locus_tag="PLF56_04920"
     CDS             complement(4490..5275)
                     /locus_tag="PLF56_04920"
                     /inference="COORDINATES: ab initio
                     prediction:GeneMarkS-2+"
                     /note="Derived by automated computational analysis using
                     gene prediction method: GeneMarkS-2+."
                     /codon_start=1
                     /transl_table=11
                     /product="hypothetical protein"
                     /protein_id="HOQ52937.1"
                     /translation="MTELGSCGVTRRPWLFLVLVLVASAPFWLLAVLTRGQGWFLGLP
                     VSVVMIIVPAAVASVLGVKEGGRTGLHALWLGVFDVARVRPGWWWVVGLGAVPVSALL
                     AWVLGARPAADSAAAVWFLAPVYFAVFYLGAIPEEVGWSSYATPPLVGRLGVLGAGGV
                     IGVVWEVWHWVPFWAQGRSWDWILSMLVFGVLSRTLMVLLYHHGGGGLAMVCAFHAML
                     NTVPLFPAGFTGFDPWLACPGMLAVLMGAMQVARFQATSPPTI"
     gene            complement(5537..6478)
                     /locus_tag="PLF56_04925"
     CDS             complement(5537..6478)
                     /locus_tag="PLF56_04925"
                     /inference="COORDINATES: protein motif:HMM:NF024109.2"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_function: GO:0016787 - hydrolase activity [Evidence
                     IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="alpha/beta hydrolase"
                     /protein_id="HOQ52938.1"
                     /translation="MILLTVAGAWSIYRTFAGEPEVGFFRSEEGRTEYVKHYSDAMAK
                     LPAPSATHDVRTDFGVVRVYEWSSPETADTVPVALVPGRSSGVPMWEANLAGFAKERR
                     VLAFDALGDAGLSVQSTPFRSFEDQAVWIDQVLAEVAPDGVHLVGHSFGGATAAAYAR
                     AHPERVHSLTLMEPVFTFGYPPVKMLWWATVATLPGMPDGIRKHALAQIGGGEDGGDD
                     PVALMIEAGSKHFSAQLPTPSTLSDAEIRRLTMPTYVAVAGADSLFGGESALDRAQEL
                     LPDGQIQVWKHATHSLPMQEAIPLDTLLDTFWAAAER"
BASE COUNT         1237 a         2346 c         2107 g         1067 t
ORIGIN      
        1 ccgcaacaat cgacaccagc caccccgagg gacgggctga cgtatagagc gctccgctct
       61 cacctcatcc gctcctctat gccgccctcc gggcgcacgg tcgctcaggt tggaatggtc
      121 gaccgccagt cccgcttgtg gagggtcggc ctcttcactg gctgccagta gcgttgttcc
      181 gtgagtgagt ggagccaaga gcaacgacac gtgagccggg cctacgacga ctggtgcctc
      241 gtagaagccg atatcagggc gttcctgaac ctgactgttc aatgggtgac atctgcccat
      301 gatgaagcgt ggattaccgc tcaggcggtg gcggaccaag tgttcgaccc cgacaagcac
      361 gacactgatc tatctgttgg cttctatgag gacaaggtga accagctctg gcgccagaac
      421 catctctgga tggtgagcgc agcagcactc aaggacgcgg tcacagcctt cgaggtctac
      481 ctcgaaatcg gtgccttgga ggccattcgc cgtcatggct tcgggtttag gacgggacgc
      541 ctcgagtctc cgtcatggtc ggacctcaag aaggtccacg agttcctcgg caccacgatc
      601 gaccccgaac ccgtgcagta cgtcaggtcg ctccgacacc ttctaactca tcagcgcggc
      661 gagctgcgca cacccgagtc acgagagcag ttcgctcacg aatccgaggg cgatcgcagc
      721 tataggcgcg aggtgacgct cgacaagcaa cgggtgactc agttcatgaa tgacttagcc
      781 acgaccgtcg cggactgcga ccgccagatt tggggaatgg catggggctc gctgcacgtg
      841 cctgacctct cgccgctctc agggcgacga ggaccacttg cagccatctc gaaacgcgcg
      901 gcgaagtagg accgactcga gtggctccgc tgtcaccgct gatgacgaca cccgtgaact
      961 ggctggccac gctcaaacgt ccgactctgc cgcgtgccgt gactcacgat gaacgacaga
     1021 ctggggacat gctgacgcca tttcccgacg gcagagggcc gatcgctgcg gggtgccgac
     1081 actcgatcgg ccgacgcatc gacggcacgg tcctgaccgc gggaaacggt gcagccggcg
     1141 aatgctcggt tgccggctgg tcggatgtgg tggccgtcgc cgtcggcaac gtccacgcct
     1201 tcagcaacac cggcaaatca cacaccctcg gcctgcgagc tgatggcact gtgctcgcca
     1261 caggatggaa cacccacggc cagtgcaacg tcggcgactg ggcgaacgtc cgcgccatcg
     1321 ctgccgggtg gcgcttctcc ctcggtgtca ccgcagacgg tcacgtggtc gccaccggcc
     1381 gcgccgcaga gggccagatc gccgtcgatg agtgggagca ggtgaggtcg gttgcctgcg
     1441 gtgactggca cagcctcggc ctccaggccg ccggccgcgt cctggctgca ggcaacaacc
     1501 gcgccgggca gctcaacgtc cacgcctggc aggacgtcgt gcaggtggcg gccggctacc
     1561 agcactccct cggtctcacc gccgatggcc gagtgattgc agcgggagcc aaggccagtg
     1621 gtgttcgcga agtcacggca tgggattcca tcgcccaggt ggcagcaggc agccaccaca
     1681 gtgtgggcct cgcccggaca gggacggtcg cggccgcggg gtccaacgcc tacggtcagt
     1741 gcaacaccca ggaatggcgc gacatcgtcg cagtcgccgc aggcgctcag cacacgctcg
     1801 gccttcgtgc ggacggcact gtcctggcta ccggcgccaa cgatcatggc cagtgtgacg
     1861 ttcacggatg ggctgacctg gcctgatccc caacgcgatc atctgtcgct ggtctcgcgc
     1921 aaggtgggtc catttctggc cgtcattccg gggtacaagt gaatagcacg tcgaggtcgt
     1981 cggtgatctt cgccgccgcc ttggccacct caccccgtac tcggcggcca gcgccttggc
     2041 tgccgcgcga gcatggtcga cgtccccggc ggcgtagatc tcggccagcg ccttcttcga
     2101 accatcatcc acgacggttc gagctcggcg cccagatgaa tcttcccctt ccggtcgccg
     2161 gacacgtgag ggcagacgag acccaccggt gacggtgcgg gcccgaacgt ccggatctgc
     2221 cgcggtgtgc gagggtagcc tttcggctgg ctgccacccg agcccgaccg ccctaaatgc
     2281 cgcggtcacg ttggcaatgg gctccttgct gggttggttt ggggcccgat gtcggtcttc
     2341 ggttcgcctg actcggctga gcgcacctcg catctctcca tgaaacggga gagcagcgag
     2401 agcgttgcga ggagttcaaa cgcttcctgc tcggtgagca caccttgcct gtgggttgcc
     2461 gggttgcgaa ccgtcatctg cacccctggc gcgaactgac gtaggccgtc ctgcatcgtc
     2521 ttcacgtcgc gatcttctgg gtcccctggc caacgaaggc gagggcgacc aggggccggc
     2581 tcgtttacag agaacagctg ctgccagatg gaggtgtcag cgatgtcatt tcgcacaccc
     2641 agccccttgg cggcgcttac gacggcgtca gctgccgcct gaacagctgc tcgcagctgg
     2701 tgcatggtcc agaatggagc ggcagcgttc catacggtgg ggtgcaacga ggctggcccc
     2761 agaaccggaa gcgccacgga cttctccgcc tcctcggcct ccgccatcat cgtggacaac
     2821 actcccaagg atcgggcgat gatccttctg agttcactcg ggtagaggaa cgccttcggt
     2881 cgcagcatcg tgtcccagtt ggagaacggg tccacgacgc cgactccctg caccaccatg
     2941 tgcgcccccg tcagctcggc caccgatgag cactccccgg cgcgccgtgc gaccgcatcc
     3001 gttctcttag cggcttcgtt cgcgtctgcc ccctctttgg gccaacacgt gggaaggtag
     3061 ccgagccctg gtccactcat gcttccctcc tcgtggagtt ccatgaatgc ctcgaacgac
     3121 tcctgaaacc gttcgagcga ttcgatggtc cggcggaggt atgcgggtgt cttgtcggtc
     3181 acatccacat cttcgcgtgt cgcagtcgat ccagaggtgc aggcaatgaa gggctaatgg
     3241 cgcatcgcgg gtctgggagg cgcactactt ccctaacgcg cgcttctgcc gcgtgcaggt
     3301 cgattgcgcg ttgaggtcgt agaagaggtc ggcgtccatc gcgggccatc gccgaggcca
     3361 acgagggact ccaacctggg caagaagtac cctgccaccg tctcgacctg ggagaacgcg
     3421 tgggaccggt tcatcccctt cctgcagttc gggcccgcgc tccgcaaggt catctacacc
     3481 accaacgcga tcgagtcgct gaactaccag ctccgcaaga tcatcaagaa ccggggccac
     3541 ttccccaacg acaccgccgc ggtcaagctg ctctggctgg cgatctgcac catcgaggac
     3601 aaaagagccc gcgaacgtgc gaaggaagcc ggcctacccg ccaccaagcg caaggccgag
     3661 gcccgactcg tggaaggcgc caccctccaa ggctggaaag ccgccctcgg ggaactcgcc
     3721 ctcgtctacc ccgaccgaat caacccctac ctctaaccca tcaaaacggg gtcttacaca
     3781 gacaactcaa caacctcgcc ctccactccc aggccggtca gccgggcggc cagggcggtg
     3841 gtccactggg tgagttcctc atccgttccc tccaccagca gggcgaatcc ccgcgggacg
     3901 gggtaccagg gccaggaccg ccgggagatc cccgtaccgg agaacccccc ttccacctcg
     3961 cgaccgacac gaacctcacc gagcccgagc cggtgtgccg tcgcaccccc gtggcggcca
     4021 caccgtgacc accgtggccc cgcgtccggc gtggaccctg ctcaccaacc acgggcacgt
     4081 gctcctggcg gtggcgggcg tgccggacgc gcgcgtcgcc gacatcgccg cagccgtggg
     4141 catcaccacc cgcgccactc tcacgattct ccacgacctc gcggaagcag gctttgttcg
     4201 ccgcacgaag atcgggcgac gcacccaccc ggcacttccg ccgccgcacc ctggccgagc
     4261 acgggattgg cgaactcctc gctctcattg cccggcacgc gcgtcccaac ctgaacaact
     4321 cggcactccc agcacaatgc ggcgttcacg acgacaccgc ccgtcgccaa gagacagccg
     4381 cgatgcccag ccagcgcttc atcgcccgac cctcgcggac ctcacgcccc cggggaaccg
     4441 cacgcccttt ccctcgcgtc cttcgagcgc cacctgagac cgccccagct cagatggtcg
     4501 gaggtgaggt cgcctggaag cgcgccacct gcatggctcc catgaggacg gccaacatcc
     4561 ccggacacgc cagccacgga tcgaacccgg tgaaccccgc cgggaacagc ggcactgtgt
     4621 tgagcatcgc gtggaaggcg caaaccatgg ccaggcctcc cccaccgtgg tggtacaaga
     4681 ggaccatcag ggttcgtgac aacaccccga agacaagcat gctcaggatc cagtcccatg
     4741 atctgccctg cgcccagaag ggcacccagt gccagacctc ccacaccaca ccgatgacgc
     4801 cccccgcccc gaggaccccc aggcggccca ccagcggggg cgtcgcatag ctggaccagc
     4861 ccacctcctc gggaatggca ccgagataga agaccgcgaa atacaccgga gccaggaacc
     4921 acacggcagc ggccgagtcc gccgccgggc gagcccccag gacccacgcc agcagcgccg
     4981 agacaggcac ggcgccgagt ccgaccaccc accaccaccc cggccgcacc ctcgcgacgt
     5041 cgaagacccc caaccaaagt gcgtgaaggc cggtcctccc accttccttg accccgagaa
     5101 cgctcgcgac cgccgcgggc acgatgatca tgacgacgct caccggcaag ccgaggaacc
     5161 aaccctgccc gcgcgtgagc accgccaaca accagaaggg cgcagatgcc accaggacga
     5221 ggacaaggaa gagccacggc cgccgagtca caccgcagga tcccagctcc gtcaccgcct
     5281 catcgtagcc aaccggtcct gcccgaacct gagccggacg aagcgaccca tccagacggt
     5341 caatccccgg ggtcaccact cctcggatgt gaatccaccc caccccgcgc tcatgctcag
     5401 acaatcccgt agcactcccc tatagcacgc tggcggtaag agcccccgcc attcatcgag
     5461 gcacgtggtt gtgtgactgc tggtggcaag cgcttggcgt gcgggagccg cgacgcttcg
     5521 tctgcgggat gctctttcag cgctcggctg cggcccagaa cgtgtcgagg agtgtgtcga
     5581 gcgggattgc ctcctgcatc ggcagggagt gggtggcgtg tttccagacc tgaatttggc
     5641 cgtcgggaag cagttcttgg gcgcggtcga gggcgctctc accgccgaag agggagtcgg
     5701 cgcccgccac ggccacgtac gtcggcatgg tgagtcgacg tatctctgcg tcggagagcg
     5761 tggagggagt ggggagctgc gcggagaaat gcttgctgcc cgcctcgatc atcagcgcga
     5821 ccgggtcatc cccgccgtcc tcgccgccac cgatctgggc gagcgcgtgt ttgcggatgc
     5881 cgtcaggcat acccgggagt gtggcgacgg tggcccacca gagcatcttg accggtgggt
     5941 acccgaacgt gaagacgggc tccatcaagg tgagcgaatg cacccgttcg ggatgcgccc
     6001 tggcgtacgc cgcagcggtg gcgccgccga aggagtgccc gacaaggtga acgccgtcgg
     6061 gggccacttc tgccaggacc tggtcgatcc agacagcctg gtcctcgaag gacctgaacg
     6121 gggtggactg gacggacagg ccggcgtccc ctagcgcgtc gaaggcgagg acccgtcgct
     6181 ccttggcgaa gccggccagg ttcgcctccc acatcggtac cccagacgac cggccaggaa
     6241 ccaaggccac gggcaccgta tcggcggtct ccggactact ccactcgtat acccggacca
     6301 ccccgaagtc ggtgcggaca tcatgcgtgg ccgacggtgc cgggagtttg gccatcgcat
     6361 cgctgtaatg cttgacgtac tcggtgcgac cctcctcgga gcggaagaag ccgacctctg
     6421 gttcgccggc aaaggtgcgg tagatactcc acgcaccggc cacggtcagc aggatcacca
     6481 ggcacttgcc cagccgacgg gtccagcggc gagcccggcg ggggcgctga tccggtgtgg
     6541 gcgcaacgac catttgttgc ggtatgtcca tactgcatag tttcacggta cggatatatt
     6601 gtaaagctgt gccgaagcga gtggaccacg aacagcgtcg agcggagatc atcgtcggtc
     6661 tgtgggcggt gatcaacggg cgggggatcg aaggggtgac ctatcaggcg gttgcacagg
     6721 cgaccgggat ctcggtgggc cggatccagc actactt
//
LOCUS       DAOSUG010000105         6750 bp    DNA     linear   ENV 10-JUN-2024
DEFINITION  MAG TPA_asm: Micropruina sp. isolate AMR_MDS_2195
            NODE_5452_length_6750_cov_4.367140, whole genome shotgun sequence.
ACCESSION   DAOSUG010000105 DAOSUG010000000
VERSION     DAOSUG010000105.1
DBLINK      BioProject: PRJNA925477
            BioSample: SAMN32864262
            Sequence Read Archive: SRR4236673
KEYWORDS    WGS; ENV; Metagenome Assembled Genome; MAG; Third Party Data; TPA;
            TPA:assembly.
SOURCE      Micropruina sp. (wastewater metagenome)
  ORGANISM  Micropruina sp.
            Bacteria; Bacillati; Actinomycetota; Actinomycetes;
            Propionibacteriales; Nocardioidaceae; Micropruina.
REFERENCE   1  (bases 1 to 6750)
  AUTHORS   Abdulkadir,N., Saraiva,J.P., Zhang,J., Stolte,S., Gillor,O.,
            Harms,H. and Rocha,U.
  TITLE     Genome-centric analyses of 165 metagenomes show that mobile genetic
            elements are crucial for the transmission of antimicrobial
            resistance genes to pathogens in activated sludge and wastewater
  JOURNAL   Microbiol Spectr 12 (3), e0291823 (2024)
   PUBMED   38289113
REFERENCE   2  (bases 1 to 6750)
  AUTHORS   Abdulkadir,N.
  TITLE     Direct Submission
  JOURNAL   Submitted (24-MAR-2023) Environmental Microbiology, Helmholtz
            Center for Environmental Research -UFZ, Permoserstr 15, Leipzig
            04318, Germany
COMMENT     The annotation was added by the NCBI Prokaryotic Genome Annotation
            Pipeline (PGAP). Information about PGAP can be found here:
            https://www.ncbi.nlm.nih.gov/genome/annotation_prok/
            
            ##Genome-Assembly-Data-START##
            Assembly Method        :: SPAdes v. 3.11.1
            Genome Representation  :: Full
            Expected Final Version :: Yes
            Genome Coverage        :: Not Applicable
            Sequencing Technology  :: Illumina MiSeq
            ##Genome-Assembly-Data-END##
            
            ##Genome-Annotation-Data-START##
            Annotation Provider               :: NCBI
            Annotation Date                   :: 05/04/2023 04:57:10
            Annotation Pipeline               :: NCBI Prokaryotic Genome
                                                 Annotation Pipeline (PGAP)
            Annotation Method                 :: Best-placed reference protein
                                                 set; GeneMarkS-2+
            Annotation Software revision      :: 6.5
            Features Annotated                :: Gene; CDS; rRNA; tRNA; ncRNA
            Genes (total)                     :: 2,776
            CDSs (total)                      :: 2,750
            Genes (coding)                    :: 2,714
            CDSs (with protein)               :: 2,714
            Genes (RNA)                       :: 26
            tRNAs                             :: 24
            ncRNAs                            :: 2
            Pseudo Genes (total)              :: 36
            CDSs (without protein)            :: 36
            Pseudo Genes (ambiguous residues) :: 0 of 36
            Pseudo Genes (frameshifted)       :: 2 of 36
            Pseudo Genes (incomplete)         :: 32 of 36
            Pseudo Genes (internal stop)      :: 2 of 36
            ##Genome-Annotation-Data-END##
FEATURES             Location/Qualifiers
     source          1..6750
                     /organism="Micropruina sp."
                     /mol_type="genomic DNA"
                     /submitter_seqid="NODE_5452_length_6750_cov_4.367140"
                     /isolate="AMR_MDS_2195"
                     /isolation_source="Activated sludge of municipal
                     wastewater treatment plants"
                     /db_xref="taxon:2737536"
                     /environmental_sample
                     /geo_loc_name="Canada"
                     /lat_lon="49.25 N 123.1 W"
                     /collection_date="2015-06-16"
                     /metagenome_source="wastewater metagenome"
                     /note="metagenomic"
     gene            <1..704
                     /locus_tag="PLF56_04930"
     CDS             <1..704
                     /locus_tag="PLF56_04930"
                     /inference="COORDINATES: protein motif:HMM:NF015000.2"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_function: GO:0008081 - phosphoric diester hydrolase
                     activity [Evidence IEA];
                     GO_process: GO:0006629 - lipid metabolic process [Evidence
                     IEA]"
                     /codon_start=3
                     /transl_table=11
                     /product="glycerophosphodiester phosphodiesterase family
                     protein"
                     /protein_id="HOQ52939.1"
                     /translation="AHRGASARQPENTIAAFTAAMGPAVDGIELDVQLSSDGVPVVIH
                     DEKVDRTHAGTGWVASHTLAQLQAMKSLLKKNPDQSITRIPTLAEVLDLLAAGQQLIN
                     LELKNSVKPYKTLEAKVLQQVAERKLGDRVVLSSFNHYSLQRLREKGWSGELAAIHDG
                     MLREPWRYFRELDVQAVHPTRHVLMVPGYVTRAHRAGFKLRVWTVNKAVQRHRCERLG
                     VDGIFTDVIKPPPSW"
     gene            839..2521
                     /locus_tag="PLF56_04935"
     CDS             839..2521
                     /locus_tag="PLF56_04935"
                     /inference="COORDINATES: protein motif:HMM:NF016357.2"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_component: GO:0016020 - membrane [Evidence IEA];
                     GO_function: GO:0047355 - CDP-glycerol
                     glycerophosphotransferase activity [Evidence IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="CDP-glycerol glycerophosphotransferase family
                     protein"
                     /protein_id="HOQ52940.1"
                     /translation="MGYDCSVEAISWERVHLTIIVRFWLTPPEVDEGFAFDQVQFSLR
                     ERQRRYPVVTHHLGEGRYQLSINIADFRSRRPVPDGTWRLIARLDGRTLKVATYPLDE
                     VETLDERSRLFLYAQNTESYTVSFGISESDVRPEFLIRTYRFFRAPLEPNSKSNSALN
                     LASDLGKRAGIALKDPRLAGVGTKVASRVLRRLFRSPGNRILFASEMRSKLEGNLVRV
                     RDRMLERGLDQEYEFTYSFRLPGVDESRFKNLKLMYRLCTSDIILVDDYFSVLEYLNI
                     PKSVKVIQLWHAGSGFKSIGYSRFGKFGSPKLHNAHRRYTYAISGSRHLVPVYAEAFG
                     IEEKAVIPTGLPRIDTFLDPVLGAKAEDEFYRVYPQAKDKRLILFAPTFRGRGISDAY
                     YDYSKLDFAQLWDFCGDDTLVLFRMHHFVDEAPPIPQQFRDRLIDVADFPETNDLLRV
                     ADVLITDYSSVIYEYSLLNRPMVFYAYDREIYAATRGFHRDFELTAPGKVCDTFDELL
                     SALREQNFDRWKIDAFTAENFDNIDTHSADRVIDWLILGDPPANVTSEANRP"
     gene            complement(2508..4454)
                     /locus_tag="PLF56_04940"
     CDS             complement(2508..4454)
                     /locus_tag="PLF56_04940"
                     /EC_number="2.4.-.-"
                     /inference="COORDINATES: protein motif:HMM:NF025025.2"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_function: GO:0016757 - glycosyltransferase activity
                     [Evidence IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="glycosyltransferase family 2 protein"
                     /protein_id="HOQ52941.1"
                     /translation="MTVSTPKVSVIIATYCSGEGLQRVIDSLDAQTLPQDEFETIFVD
                     DGSPDDTYAALQKLASTRANMKVLRIENSGWPSRPRNVGMQHATGDYLFFMDHDDSLY
                     PDGLRRAHEFAVETDADIVSTKESKTNDSWWGMSSLTQGNTSHVKRHGGIWLLLPLVP
                     HKLYRRSLIEERHILFPDGRAVLWEDQFFNVAAYSAAKEVAVLADSPTYLWHASDTNT
                     SHTFSPSTENFWLMLEKLLQFTVETLSSDELRDDRDTLLAHHMKTRVIDRTVRQLISG
                     PEESGQLALTRARRLQSRYLADGVFHRLPKKHQSQALLLRMGASGALRKMHRLDLKGR
                     GVTRVLRARWEGHAMQLEMETTWSSKKPEEPILERTDDGRVLRAVGSELRSLLPSHLY
                     DLTADPPRLMTSVILRARTSFVSWEVALESKSASFVDVESNPAARTFLEACVDVRTAE
                     LGRELAEDVWDLRYTADWVGMTRIGPVGIRTKPLPALIDQRVYIAYCNAKGGLTIDMT
                     QHLRCLVTDAEPRLGPIGEADAIRMELQNLHIVGESRLDDTRVALARLDEDLGEQGVV
                     LTLDDTRVAPIEGHVQAGADGAQVLGAVRQHLEAGGYALLANRENAWRSTRYCVEVSP
                     DGSLELRLAQSAPAEAEPTATTVG"
     gene            4603..5511
                     /locus_tag="PLF56_04945"
     CDS             4603..5511
                     /locus_tag="PLF56_04945"
                     /EC_number="4.1.3.36"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_013865912.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_function: GO:0008935 - 1,4-dihydroxy-2-naphthoyl-CoA
                     synthase activity [Evidence IEA];
                     GO_process: GO:0009234 - menaquinone biosynthetic process
                     [Evidence IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="1,4-dihydroxy-2-naphthoyl-CoA synthase"
                     /protein_id="HOQ52942.1"
                     /translation="MTNPWRPGLWNEVPGFEFTDITYHRAKDVPAVRIAFDRPEVRNA
                     FRPHTVDELYIALDHARRSADVGCVLLTGNGPSAKDGGWAFCSGGDQRIRGRSGYQYA
                     QGETSETVDARRAQAEGGRLHILEVQRLIRFMPKVVIALVNGWAAGGGHSLHVVCDLT
                     IASAEHAKFKQTDADVGSFDAGFGSAYLARQVGQKVAREIFFLGQTYDAQRAYEMGTV
                     NKVVPHADLETVGLEWASLICGKSPTAQRMLKFAFNAIDDGLIGQQVFAGETTRLAYM
                     TDEAVEGRDSFLQKRPPDWSAFPYYY"
     gene            complement(5840..6688)
                     /locus_tag="PLF56_04950"
     CDS             complement(5840..6688)
                     /locus_tag="PLF56_04950"
                     /EC_number="1.6.5.2"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_008357610.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_function: GO:0016655 - oxidoreductase activity, acting
                     on NAD(P)H, quinone or similar compound as acceptor
                     [Evidence IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="SDR family oxidoreductase"
                     /protein_id="HOQ52943.1"
                     /translation="MPGPLIGITGATGHIGRLVATRLAGTGARLRLLARDPSRLPTLN
                     APVQVARTSYEDTAEARAALAGVEVLFMVSAAESADRLAQHTGFLDAAAAAGVRHVVY
                     TSFLGAAPQATFTLARDHWVTEEHLRSLGLAWTVLRDSFYLDFLPDLADPDGAIRGPA
                     GTGRVGAVARADVARVAAAVLSAPESHAGAVHEVTGPEALTLAEVAETITRVTGRSTT
                     YVDETIEQAYASRAVYHAADWQVDAWVSTYTAIAKGELATLTGTVQRIGGQPPLSLAE
                     LLRSRS"
BASE COUNT         1172 a         2124 c         2216 g         1238 t
ORIGIN      
        1 gggcacaccg cggagccagc gcgcggcagc cggaaaacac gatcgccgcg ttcaccgcgg
       61 cgatggggcc cgccgtcgac ggcatcgaac tcgatgtgca gctgagttcg gacggcgtgc
      121 cggtggtgat ccacgacgag aaggtggacc gcacgcatgc gggcaccggc tgggtggcca
      181 gccacacgct cgcgcaactg caggcgatga aatcgttgct gaagaagaac cccgatcagt
      241 ccatcacgcg cattccgacc ctggccgagg tgctcgacct gctcgcggcc ggccagcagc
      301 tgatcaacct cgaactgaag aacagcgtca agccctacaa gacgctggag gccaaggtgc
      361 tgcagcaggt cgccgaacgc aagctcggcg accgggtggt gctctcgtcg ttcaatcatt
      421 actcgctgca acgcctgcgc gagaaggggt ggagtggtga gttggcggcg attcacgacg
      481 gcatgctgcg tgaaccctgg cggtatttcc gagagctgga cgtgcaggcg gtgcacccga
      541 cccggcacgt gctgatggtg cccgggtacg tcacccgggc gcaccgggcg ggcttcaagc
      601 tgcgggtctg gacggtgaac aaggccgtgc agcggcaccg gtgcgagcgg ctgggcgtgg
      661 acggcatctt caccgacgtg atcaagccgc caccgagctg gtgaggtggc cgacaggtcg
      721 cctgggcccg cgggttccgg ctgacgtcac agggcgcgcc gccgttgttg tcgacctgtg
      781 ttcacctagg cgttaacctc gaacacaagt aactgcagtc aaccgtattg gagctgggat
      841 gggctacgac tgttcggtcg aagcgatttc ctgggaacgg gtccatctca caatcatcgt
      901 caggttctgg ctgacgccgc ccgaagtcga tgagggtttc gccttcgatc aggtgcagtt
      961 ctcgttgcgc gagcgtcagc gccgctaccc cgtggtcacc catcatctgg gcgagggccg
     1021 ctatcaactc tcgatcaaca ttgccgattt ccgctcgcgg cgtccggtgc cggacggtac
     1081 ctggcgcctg atcgcccgac tggacggccg aacgctcaag gtggccacct accccctcga
     1141 cgaggtcgag acgctggacg agcggtcgcg gctgtttctg tacgcgcaga acacggagag
     1201 ctacacggtc tctttcggaa tatccgaaag cgacgtgcgg cccgagtttc tgattcgtac
     1261 ctaccggttc tttcgggcgc cgctcgaacc gaactcgaag tcgaactctg cgctgaacct
     1321 ggcctccgac ctgggcaagc gggccggcat cgccctgaag gatccccggc tggccggcgt
     1381 gggcacgaaa gtcgcctcac gggtgcttcg acgactgttt agatcgccgg gcaatcgcat
     1441 cctgttcgcc tcagagatgc gcagcaaact cgagggcaac ctggtgaggg tgcgtgaccg
     1501 catgctcgag cgggggctgg atcaggaata cgagttcacc tattcgtttc ggctgccggg
     1561 ggtcgacgaa tcgcgcttca agaatctgaa gctgatgtac cggctgtgca cctccgacat
     1621 cattctggtc gacgactact tcagcgttct cgaatacctg aacattccga aaagcgtcaa
     1681 ggtcattcag ttgtggcacg ccggcagcgg tttcaagtcc atcggctaca gccgattcgg
     1741 caagttcgga tcaccgaagt tgcacaacgc ccaccgtcgc tacacctacg cgatctcagg
     1801 ttcgcggcac ctggtgcccg tgtacgccga ggctttcggc atcgaagaga aggcagtgat
     1861 tcccaccggc ctgccacgca tcgacacctt cttggatccg gtcctcggtg cgaaggccga
     1921 ggacgagttc taccgggtgt acccgcaggc caaggacaag cgtctcattc tgttcgcgcc
     1981 cacctttcga ggtcggggca tctcggacgc ctattacgac tacagcaaac ttgattttgc
     2041 gcagttatgg gacttctgcg gtgacgacac gttggtgctt tttcgcatgc atcatttcgt
     2101 cgacgaggct ccgccgatcc cgcagcagtt tcgcgaccgg ctcatcgacg tggccgattt
     2161 tcctgagacg aacgacttgc tcagggtggc cgacgtgctg atcaccgact actcgtcggt
     2221 gatctacgaa tactcgctac tgaatcgccc gatggttttc tacgcttacg atcgtgagat
     2281 ctatgcggcc acgaggggct ttcatcgaga cttcgaactg accgccccgg gtaaggtgtg
     2341 cgacaccttc gacgaactgc tcagcgcgct gcgcgagcag aacttcgatc ggtggaagat
     2401 cgacgccttc accgctgaga acttcgacaa tatcgacacc cactcggccg accgggtgat
     2461 cgattggctg atcctgggcg atccgcccgc caacgtgacg agtgaggcta accgaccgta
     2521 gtcgccgtcg gctcggcctc ggcaggtgcc gactgcgcca gcctcaactc cagcgacccg
     2581 tcgggcgaca cctcaacgca gtagcgcgtg gagcgccagg cgttctcacg gttggccaac
     2641 agcgcataac cgcccgcctc gaggtgctgc cgcaccgcgc ccaggacctg tgcaccgtcc
     2701 gctcccgcct gaacatggcc ttcgatcgga gcaaccctgg tgtcgtccag tgtcagcacg
     2761 acgccctgtt cgccgaggtc ctcgtccaac cgggccagcg ccacgcgggt gtcgtcgagt
     2821 cgggactcgc cgacgatgtg caggttctgc aattccatgc ggatagcgtc ggcctcgccg
     2881 atcgggccca gccggggctc ggcgtccgtg acgaggcagc gcaggtgttg cgtcatgtcg
     2941 atggtgaggc ctcccttcgc gttgcagtac gcgatgtaca cccgctgatc gatcaaggcg
     3001 ggtagaggtt tcgttcgtat tcctaccgga ccaatccgcg tcatcccaac ccaatccgcc
     3061 gtgtagcgaa ggtcccatac atcttcggca agttcacgcc cgagttcggc tgtgcgcaca
     3121 tcgacgcaag cttccagaaa tgtccgggct gccgggttcg actcgacgtc gacgaaggag
     3181 gccgacttgc tctcgagcgc cacttcccag gaaacgaagc tcgtccgcgc ccggaggatc
     3241 acactcgtca tcaggcgagg agggtcggca gtaaggtcat acaggtgtga gggcagaagg
     3301 gagcgcagtt ccgagcctac ggcgcggagt acacggccat catcggtgcg ctcgagaatc
     3361 ggctcctcgg gcttctttga tgaccaagtc gtctccatct caagttgcat tgcatgaccc
     3421 tcccagcgag cgcgcagcac gcgggtcaca ccccggcctt ttaggtccag tcgatgcatc
     3481 ttgcgcaatg cgccgcttgc tcccatgcgt aataacaacg cctgcgactg gtgcttcttc
     3541 ggcaggcgat gaaacactcc gtcggcgaga tagcgtgatt gcagcctgcg agcgcgagtg
     3601 agtgcgagct ggccagattc ctcgggacca ctgatgagtt gacggacggt ccggtctatc
     3661 acgcgggtct tcatgtggtg ggcgagcagt gtatcccggt cgtcacggag ttcatcactg
     3721 gatagagtct caacagtgaa ctgcaagagc ttctccagca ttagccagaa gttctctgtc
     3781 gacggactga aggtatgtga tgtgttggta tcgcttgcgt gccacaggta cgtgggggaa
     3841 tcggccaaga cggccacttc cttggcagca ctgtatgcgg cgacattaaa aaactgatcc
     3901 tcccaaagta ctgctctgcc gtccgggaat agtatgtgcc gttcctctat caaggagcgt
     3961 cggtatagtt tgtgcggtac caagggaagg agcagccata ttccgccatg acgctttaca
     4021 tgtgaggtgt ttccttgagt taggctcgac attccccacc acgagtcgtt cgtcttggac
     4081 tctttggtgc tgacgatgtc ggcgtcggtc tcgacggcga actcgtgggc acggcgcagg
     4141 ccgtcggggt agagcgagtc gtcgtggtcc atgaagaaca ggtagtcacc ggtggcgtgc
     4201 tgcatgccca cgttgcgcgg acggctcggc cagccggagt tctcgatccg gagaactttc
     4261 atgttcgcac gggtggacgc cagcttctgc agtgccgcat acgtgtcgtc cggcgatccg
     4321 tcgtcgacga agatggtctc gaactcgtcc tgcggcaggg tttgtgcgtc gagtgaatcg
     4381 atgactcgct gaaggccctc gcccgagcag taggtggcga tgatgacgct caccttcggt
     4441 gtactgacgg tcacgcgatc tcctacctca aaaccatggg cggcggccat gatcttacgc
     4501 gtggggcctc gacccgccgg gcaggctggc gggtgacgac gcgacgggga gcgctcaccg
     4561 ggcccgtccc ccgcctatcg acggaccgtt agtctgagcc ccatgaccaa cccgtggcgt
     4621 cctggactgt ggaacgaggt tcccggcttc gagttcaccg acatcaccta ccaccgggcc
     4681 aaggacgtgc ccgcggtacg gatcgcgttc gaccggcctg aggtgcgcaa cgcctttcgt
     4741 ccgcacacgg tggacgagct gtacatcgca ctcgaccacg cgcgacgctc cgcggacgtg
     4801 gggtgtgtgc tgctcaccgg caacggcccg tccgccaagg acggcgggtg ggccttctgc
     4861 tccggcggcg accagcggat ccggggccgc tccggctacc agtatgcgca gggcgagacg
     4921 tccgagacgg tggacgcgcg acgcgcgcag gctgagggcg ggcggctgca cattctcgag
     4981 gtgcagcggt tgatccggtt catgcccaag gtggtgatcg ccctggtcaa cggctgggcc
     5041 gccggcggcg gccactcgct gcatgtggtc tgcgacctga ccatcgcctc ggccgagcac
     5101 gcgaagttca agcagaccga cgccgacgtc ggctcgttcg acgccggatt cggctcggcg
     5161 tacctggccc gccaggtggg ccagaaagtc gcacgtgaga ttttctttct cggccagacc
     5221 tacgacgccc agcgcgctta tgagatgggc accgtcaaca aggtcgtgcc gcacgccgac
     5281 ctcgaaacgg tcgggcttga gtgggcgtcg ttgatctgcg gcaagtcgcc gaccgcccag
     5341 cgcatgctca agttcgcgtt caacgcgatc gacgacggcc tgatcggcca gcaggtgttc
     5401 gccggcgaga ccacccggct cgcctacatg accgacgagg ccgtcgaggg acgcgactcc
     5461 tttctgcaga agcgtccgcc ggactggtcg gcatttccgt actactactg aagcagccct
     5521 ataggcgcta gtcagcacac tttgaagtcc cgctggtccg cacagagtcc gcaggaagta
     5581 gagccatcca ggatcgcggc caggctcacg acgctggtcg agtggcctga ctcgaattcg
     5641 tcgaagtcga catcccacgt tcgtcggtca tgaagttgga gatccgtcga cgtccgcgcg
     5701 tcgggtcgga caccgcgatc caccccggtg gccgtcccct ctcgtcgaac gctggaccgt
     5761 gcggcggtcg gggaggcgac cgccggagcc ttcaagctcg tgcctgtgcg ctatagcccg
     5821 gccggcgaga ggggggccgt cacgaccggc tgcgcagcag ttcggccagg ctgagtggtg
     5881 gctgtccgcc gatgcgctgg acggtgccgg tgagcgtggc cagttcgccc ttggcgatgg
     5941 cggtgtaggt ggacacccag gcgtccacct gccagtcggc agcgtggtag acggctcggg
     6001 acgcgtaggc ctgttcgatg gtttcatcga cgtaggtggt ggagcggccc gtgactcggg
     6061 tgatggtctc ggcgacctcg gccagggtga gtgcctccgg cccggtcact tcgtggacgg
     6121 cgccggcgtg cgattccggg gcgctgagca cggcggcggc gacgcgggcg acgtcggcac
     6181 gggcgaccgc gcccacccgt ccggtgccgg cggggccacg gatcgcgccg tccgggtcgg
     6241 ccaggtcggg cagaaagtcc aggtagaacg agtcccgtag caccgtccag gccaggccca
     6301 aggagcgcag gtgttcctcg gtgacccagt ggtcgcgggc gagggtgaac gtggcctgcg
     6361 gggcagcgcc caggaacgag gtgtacacca cgtggcggac gccggctgcc gcggcggcat
     6421 cgagaaagcc ggtgtgctgg gcgaggcggt cggcgctctc ggcggccgac accatgaaca
     6481 gcacctcgac cccggccaag gccgcccgtg cttcagcggt gtcttcgtag ctggtccggg
     6541 ccacctgcac cggggcattc agagtgggca gcctggacgg gtcgcgggcc agcagccgca
     6601 gccgggcgcc ggtgccggca agccgcgtgg cgacgaggcg gccgatgtgt ccggtggccc
     6661 cggtgattcc gatcagtggt ccaggcatgt ctcgggtccc tcctcgaagt gctcgttgcg
     6721 atccagtcag caaactagac cgtgtgtcgc
//
LOCUS       DAOSUG010000106         6723 bp    DNA     linear   ENV 10-JUN-2024
DEFINITION  MAG TPA_asm: Micropruina sp. isolate AMR_MDS_2195
            NODE_5471_length_6723_cov_4.203359, whole genome shotgun sequence.
ACCESSION   DAOSUG010000106 DAOSUG010000000
VERSION     DAOSUG010000106.1
DBLINK      BioProject: PRJNA925477
            BioSample: SAMN32864262
            Sequence Read Archive: SRR4236673
KEYWORDS    WGS; ENV; Metagenome Assembled Genome; MAG; Third Party Data; TPA;
            TPA:assembly.
SOURCE      Micropruina sp. (wastewater metagenome)
  ORGANISM  Micropruina sp.
            Bacteria; Bacillati; Actinomycetota; Actinomycetes;
            Propionibacteriales; Nocardioidaceae; Micropruina.
REFERENCE   1  (bases 1 to 6723)
  AUTHORS   Abdulkadir,N., Saraiva,J.P., Zhang,J., Stolte,S., Gillor,O.,
            Harms,H. and Rocha,U.
  TITLE     Genome-centric analyses of 165 metagenomes show that mobile genetic
            elements are crucial for the transmission of antimicrobial
            resistance genes to pathogens in activated sludge and wastewater
  JOURNAL   Microbiol Spectr 12 (3), e0291823 (2024)
   PUBMED   38289113
REFERENCE   2  (bases 1 to 6723)
  AUTHORS   Abdulkadir,N.
  TITLE     Direct Submission
  JOURNAL   Submitted (24-MAR-2023) Environmental Microbiology, Helmholtz
            Center for Environmental Research -UFZ, Permoserstr 15, Leipzig
            04318, Germany
COMMENT     The annotation was added by the NCBI Prokaryotic Genome Annotation
            Pipeline (PGAP). Information about PGAP can be found here:
            https://www.ncbi.nlm.nih.gov/genome/annotation_prok/
            
            ##Genome-Assembly-Data-START##
            Assembly Method        :: SPAdes v. 3.11.1
            Genome Representation  :: Full
            Expected Final Version :: Yes
            Genome Coverage        :: Not Applicable
            Sequencing Technology  :: Illumina MiSeq
            ##Genome-Assembly-Data-END##
            
            ##Genome-Annotation-Data-START##
            Annotation Provider               :: NCBI
            Annotation Date                   :: 05/04/2023 04:57:10
            Annotation Pipeline               :: NCBI Prokaryotic Genome
                                                 Annotation Pipeline (PGAP)
            Annotation Method                 :: Best-placed reference protein
                                                 set; GeneMarkS-2+
            Annotation Software revision      :: 6.5
            Features Annotated                :: Gene; CDS; rRNA; tRNA; ncRNA
            Genes (total)                     :: 2,776
            CDSs (total)                      :: 2,750
            Genes (coding)                    :: 2,714
            CDSs (with protein)               :: 2,714
            Genes (RNA)                       :: 26
            tRNAs                             :: 24
            ncRNAs                            :: 2
            Pseudo Genes (total)              :: 36
            CDSs (without protein)            :: 36
            Pseudo Genes (ambiguous residues) :: 0 of 36
            Pseudo Genes (frameshifted)       :: 2 of 36
            Pseudo Genes (incomplete)         :: 32 of 36
            Pseudo Genes (internal stop)      :: 2 of 36
            ##Genome-Annotation-Data-END##
FEATURES             Location/Qualifiers
     source          1..6723
                     /organism="Micropruina sp."
                     /mol_type="genomic DNA"
                     /submitter_seqid="NODE_5471_length_6723_cov_4.203359"
                     /isolate="AMR_MDS_2195"
                     /isolation_source="Activated sludge of municipal
                     wastewater treatment plants"
                     /db_xref="taxon:2737536"
                     /environmental_sample
                     /geo_loc_name="Canada"
                     /lat_lon="49.25 N 123.1 W"
                     /collection_date="2015-06-16"
                     /metagenome_source="wastewater metagenome"
                     /note="metagenomic"
     gene            complement(<1..2191)
                     /locus_tag="PLF56_04955"
     CDS             complement(<1..2191)
                     /locus_tag="PLF56_04955"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_007078151.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_function: GO:0016624 - oxidoreductase activity, acting
                     on the aldehyde or oxo group of donors, disulfide as
                     acceptor [Evidence IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="thiamine pyrophosphate-dependent enzyme"
                     /protein_id="HOQ52944.1"
                     /translation="MPIIEPLEPSASWNQFTATADDWDAADPALLASMTVQMHLIRAF
                     EEQVLDLAGQKLINGPAHSSIGQEGGAVGSAIPLTVEDQINGSHRGHHQFLAKALHAV
                     QPDGFSIGDDWTPEVVTVLRRTLSEIAGLSEGYCRGRGGSMHLQWKEVGAMGTNAIVG
                     GAVPFATGFAMAHKLAKTDAVAVTYFGDGASNIGSTLEALNIAAAWKLPIVFFIENNQ
                     YAVSTTVHESTADPRLSVRGLGFGIPSWRVDGQDALAVKLLMDEVLAHVRSGEGPAVV
                     EADTYRFFHQNGPFPGSAFGYRSKEEEAGWRARDPLQLAEAHLERLDIATREQLDALR
                     KQCKAVMQQLSDTITEPDPAGRPGQLRIRPELWPSASFIDVGIRGEWPDAGDRVRTEA
                     DFTDDELVKQRFVDTIAAVMHRRMETDDSVIVLGEDVHKLNGGSRGATKGLPQDFPGR
                     VLGTPIAEAGFTGLGGGVALDGRFYPVVELMYADFIWVAADQIFNQIGRARHMFGGDH
                     DMAMLMRLKIGTTTGYGSQHSMDPAGILSNSVGWRIIAPSTPLDYVGLLNAAMLTRDP
                     VAVLEHDADLYKLEGLAPTDLDFVLPFGKAAVRREGAEVTLISYLSMVQRCLDAVEQS
                     GVDGEVIDLRWLDRASLDWQTIEASVKKTNRVVIVEQGAQGTSYGGWLAGEISRRLFD
                     WLDAPVLRVQGGEAAPSISKALETAANANTDDIVAALARERLHAEEDP"
     gene            2334..3110
                     /locus_tag="PLF56_04960"
     CDS             2334..3110
                     /locus_tag="PLF56_04960"
                     /inference="COORDINATES: protein motif:HMM:NF019322.2"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_function: GO:0003677 - DNA binding [Evidence IEA];
                     GO_function: GO:0003700 - DNA-binding transcription factor
                     activity [Evidence IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="GntR family transcriptional regulator"
                     /protein_id="HOQ52945.1"
                     /translation="MTTTLNRNSAVPLYLQLADELRHKIVSGEWQPEQRIPSENELNQ
                     IYSISRMTIRQVLGKLVDEGLVFRVHGKGTFVAQTKIPTRSPSYHGVREQLEQQGYST
                     RTETLAAEVIEADDRLARQLDVPLHTPLSMVERLRFAGETPISLHKSYVPVPLAPGLL
                     DHDLAKRQLCQVLAQDYGLNMAYVHESLESLTPTTHELAALNVRRGTALLLLEQRISD
                     HSRRLFEYTRIKFRGDRVRLEFDYDLTEVQPGAARQPAFG"
     gene            3186..3419
                     /locus_tag="PLF56_04965"
     CDS             3186..3419
                     /locus_tag="PLF56_04965"
                     /inference="COORDINATES: protein motif:HMM:NF039180.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="DUF5652 family protein"
                     /protein_id="HOQ52946.1"
                     /translation="MSMSHKHWSELSPLKRVAIVKLVVLDLGLRTWALIELAHRPKNQ
                     VRGSKTVWALALTLVSSVGILPTIYLTRARKTD"
     gene            complement(3453..4781)
                     /locus_tag="PLF56_04970"
     CDS             complement(3453..4781)
                     /locus_tag="PLF56_04970"
                     /inference="COORDINATES: protein motif:HMM:NF025065.2"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="iron-containing alcohol dehydrogenase"
                     /protein_id="HOQ52947.1"
                     /translation="MDDFAREVSQGALSHLATGPAALDGLSSQCRLLAPSGERVLLLD
                     DVPKAAAGRDLVAWVSELVGEVRMVVVRATRAQVRLDEATIESTVAEVGRPALIVSLG
                     SGTVTDLGKVVSARSGAPLVAVQTAASVNGFSDPLSVLVIGGAKRTKPSAWPSALIID
                     DRLVDAAPLRLNRSGVGDAIAIWSAPADWYLACALGLDGPFLDEAVRPVLEAVRGLAD
                     PDPDVARRALVESLTVGGLVIGIADSTAPLSGSEHLTSHILDMAAMARGVEHDLHGAQ
                     VGVATVVCTALWAEVRESCTVFEVPWAGLRYPDDLEQRVRETWAAIDPSGALGEECWT
                     AVDRKAAAWNAADKGECDRNRADVLADLFGYVGDPIRAAGTLRHWGASTRFSELDSPV
                     GRDDVLWTLRALPFMRDRLTLPDLLVMTGEWTDELIERVLARAQAAGGGA"
     gene            complement(4791..6338)
                     /locus_tag="PLF56_04975"
     CDS             complement(4791..6338)
                     /locus_tag="PLF56_04975"
                     /EC_number="2.7.1.-"
                     /inference="COORDINATES: protein motif:HMM:NF012589.2"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_function: GO:0005524 - ATP binding [Evidence IEA];
                     GO_function: GO:0019200 - carbohydrate kinase activity
                     [Evidence IEA];
                     GO_process: GO:0016310 - phosphorylation [Evidence IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="FGGY-family carbohydrate kinase"
                     /protein_id="HOQ52948.1"
                     /translation="MPQYLLGIDAGTSVIKAAIFDEHGNELSRGAHNVPIDNPTTYMA
                     EEDMETVWVAATLAIRDAIEGSAVHASDIAAISATGQGDGTWLVDAEGEPAGPAFLWT
                     DGRAADIIDTWYADGTISKQFQISGLGPYAGTTSALLRWRLENQPEILQGATNLWCKD
                     WVEYKLTGDLSTDPSDASLGGIDVRTRDWSDEVLTHLGLTEAKSVLPVIKSPITQIGS
                     ITAEAAGLTGLAEGTPVFKGQMDITASSLGVGVARPGDCMAVIGTAGIVTVATEDLGD
                     TIEPDDVGWVIPHSPTTWIRALGMSSCTPNVDWYLREFGGSFRREAEARGPQYTFFEY
                     LDEVVKGVPVGSRGVMFHGYTAPGGERAPFVKPSARASFIGITGAHTRNDLLRAVYEG
                     VAYGIRDCLDAIPTEVTVVRMAGGGANSPVWAQIFADVLGRKVVVPAGTEFGAKGAAI
                     VAGVGVGMFDSYEQGADSTVNILRKYKPKKKKTDLYDEYFAIYKEYRQAMPPIWDKLQ
                     AITRREG"
     gene            6463..>6723
                     /locus_tag="PLF56_04980"
     CDS             6463..>6723
                     /locus_tag="PLF56_04980"
                     /inference="COORDINATES: protein motif:HMM:NF019845.2"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="alcohol dehydrogenase catalytic
                     domain-containing protein"
                     /protein_id="HOQ52949.1"
                     /translation="MRALVKYAPGPGNVELRDVPRPRPGPGQVLIEVAFAGVCGTDRS
                     ALDGHFAGLIPRTLGHEASGIIAELGPDLRRSDLAVGDRVTFE"
BASE COUNT         1067 a         2337 c         2266 g         1053 t
ORIGIN      
        1 ccggatcctc ctcggcgtgc agccgctcgc gggccagcgc ggcgacgatg tcgtcggtgt
       61 tggcgttggc ggcggtctcc agcgccttgc tgatgctcgg cgcggcctcg ccgccctgca
      121 cccgcagcac cggcgcgtcc agccagtcga acagccgccg cgagatctcg ccggccagcc
      181 agccgccgta gctggtgccc tgggcgccct gctcgacgat caccacccgg ttggtcttct
      241 tcaccgaggc ctcgatggtc tgccagtcca gggacgcccg gtcgagccag cgaaggtcga
      301 tcacctcacc gtccacaccg gactgctcga ccgcgtccag gcagcgctgc accatcgaca
      361 ggtagctgat cagagtgacc tcggcgcctt cgcggcgtac cgcggccttg ccgaacggca
      421 gcacgaaatc gaggtccgtg ggtgccagac cttccagctt gtacaggtcg gcgtcgtgtt
      481 cgagcaccgc gaccgggtcg cgagtcagca tcgcggcatt cagcaggccc acgtagtcga
      541 gcggggtgga cggggcgatg atcctccagc cgaccgagtt cgacaggatg ccggccggat
      601 ccatcgaatg ctgcgagccg taacccgttg tcgtgccgat cttgagccgc atcagcatgg
      661 ccatgtcgtg gtcgccgccg aacatgtgcc gggcgcgtcc gatctggttg aagatctggt
      721 cggccgccac ccagatgaag tcggcgtaca tgagttcgac caccgggtag aaccggccgt
      781 ccaacgcgac gccgccgccc aggccggtga acccggcctc ggcgatcggg gtgccgagca
      841 cccggccggg aaagtcctgc ggcaggccct tggtggcacc gcgcgagccg ccgttgagct
      901 tgtggacgtc ctcgccgagc acgatcaccg aatcgtcggt ctccatgcgg cgatgcatca
      961 ccgccgcgat ggtgtcgacg aaacgctgct tgaccagctc gtcgtcggtg aagtcggctt
     1021 cggtgcgcac ccggtcgccc gcgtcgggcc actcgccgcg gatgccgacg tcgatgaagg
     1081 acgccgaggg ccacagttcg ggccggatgc gcagctgccc gggacgtccg gccgggtcgg
     1141 gctcggtgat cgtgtccgac aactgctgca tgaccgcctt gcactgcttg cgcagggcgt
     1201 ccagctgctc gcgggtggcg atgtcgagcc gctccaggtg cgcttcggcc agctgcagcg
     1261 ggtcacgggc gcgccagccg gcctcctcct ccttgctgcg gtagccgaag gcgctgccgg
     1321 ggaacgggcc gttctggtga aagaaccggt aggtgtcggc ctcgaccacg gccgggccct
     1381 cgccgctgcg gacgtgcgcg agcacctcgt ccatcagcag tttgacggcc agcgcgtcct
     1441 gcccgtccac ccgccagctt gggatgccga agccgagacc gcgcaccgag agccgcgggt
     1501 cggcggtgga ctcgtggacg gtggtcgaca ccgcgtactg gttgttctcg atgaagaaga
     1561 cgatcggcag cttccaggcg gccgcgatat tcagcgcctc gagtgttgag ccgatgttgg
     1621 acgccccgtc gccgaagtag gtgaccgcga ccgcgtcggt cttggccagc ttgtgcgcca
     1681 tcgcgaaccc ggtggcgaag ggaacggcgc cgccgacgat ggcgttggtg cccatcgccc
     1741 cgacttcttt ccactgcagg tgcatcgagc cgccgcggcc gcggcagtaa ccctcggaca
     1801 ggccggcgat ctcggacagc gtgcggcgca gcacggtgac cacctcgggg gtccagtcgt
     1861 cgccgatcga gaagccgtcg ggttgcaccg cgtggagggc cttcgccaag aactggtggt
     1921 ggccgcggtg cgagccgttg atctgatcct cgacggtgag cggaatcgcg gagccgaccg
     1981 cgccgccctc ctggccgatc gagctgtggg ccggaccgtt gatcagcttc tgtcccgcca
     2041 gatcgagcac ctgctcctcg aacgcgcgga tcaggtgcat ctgcacggtc atcgacgcca
     2101 ggagtgccgg gtcggccgcg tcccagtcgt cggcggtcgc cgtgaactga ttccagctgg
     2161 ccgacggctc aaggggctcg ataatcggca tggctacatt gcctcctcgg tgctgcgttg
     2221 tcctggcggc ctcagcgcga ccgctggggt aggctcaggc tagccaagct actgcacatg
     2281 accatacatg tacagtcaag ttggccgcat cgacgaacgg cagagaggac agcgtgacca
     2341 ccaccttgaa ccgcaactcc gccgttccgc tgtacctgca actcgccgat gagttgcgtc
     2401 acaaaattgt tagcggtgaa tggcagccgg agcaacgcat accgtccgaa aacgagctga
     2461 atcagatcta ctcgatcagc cgcatgacga tccgccaggt actaggcaag ctcgtggacg
     2521 aggggctggt gttccgcgtg cacggcaagg gcacgttcgt ggcacagacc aagatcccga
     2581 cccgttcgcc cagctaccac ggagtgcgcg aacagttgga gcagcagggc tactccactc
     2641 gcaccgaaac ccttgccgcc gaggtgatcg aggcggacga caggctcgcg cgtcagctcg
     2701 acgtgccgct gcacaccccg ctgtcgatgg tcgagcggct gcggttcgcc ggcgagacgc
     2761 cgatcagcct gcacaagtcg tacgtgccgg tgccactggc gcccggcctg ctcgaccacg
     2821 acctcgcgaa gcgacagttg tgccaggtgc tggcgcagga ctacgggctg aacatggcct
     2881 acgtgcacga gagcctcgaa tcgctcacgc cgaccacgca cgaactggcc gcactgaacg
     2941 tcaggcgcgg caccgcgctg ctcctgctcg agcaacggat cagcgaccac tcccggcggc
     3001 tgttcgagta cacccggatc aagttccgcg gtgaccgcgt gcggctcgaa ttcgactacg
     3061 acctgaccga ggtgcagccg ggcgcggcca gacaaccggc cttcgggtaa aacccaccgg
     3121 tcgagcttgt cgagaccacg aacgtcgaga ccggggtagc gtcgacgtca acgtgagagg
     3181 gcgtgatgag catgtcgcac aagcactgga gcgaactgtc gccactgaaa cgtgttgcga
     3241 tcgtgaagtt ggtcgtgctg gatctcgggt tgcggacctg ggcactgatc gaactggcgc
     3301 accgtcccaa gaatcaggtg cgtggctcga agacggtctg ggcgctcgcg ctcaccctgg
     3361 tgagttcggt gggcattctg ccgacgatct acctgaccag ggctcgcaaa accgactgag
     3421 ggctggccca tcgcgccgag atcagcgctt cgctaagccc cgccgcccgc ggcctgcgct
     3481 cgcgccagca cccgctcgat cagctcgtcg gtccactccc cggtcatcac cagcaggtcg
     3541 ggcagggtca gccggtcgcg catgaagggc agggcgcgca acgtccacag cacgtcgtcg
     3601 cggccgaccg gggagtcgag ttcgctgaac cgggtggacg ccccccagtg ccgcagggtg
     3661 cccgcagcgc gtatggggtc cccgacgtag ccgaacaggt ccgccagcac atcggcccga
     3721 ttgcggtcgc attcgccctt gtcggcggcg ttccaggcgg ccgccttgcg gtcgacggcc
     3781 gtccagcact cctcacccaa cgctccggac gggtcgatcg ccgcccaggt ctcgcgcacc
     3841 cgctgctcca ggtcgtcggg gtagcgcagc cccgcccagg gcacctcgaa caccgtgcac
     3901 gattcgcgca cctcggccca caacgcagta cagaccaccg tcgcgacgcc cacctgggcg
     3961 ccgtgcaggt cgtgctcgac gccacgagcc atcgccgcca tgtcgagaat gtggctggtc
     4021 aggtgctccg atcccgacag cggagccgtc gagtcggcga tgccgatcac cagcccgccc
     4081 acggtcaacg actcgaccag agcccgacgg gcgacgtccg ggtcggggtc ggcaaggccg
     4141 cgcaccgcct ccagcaccgg gcggacggcc tcgtccagga agggtccgtc caggcccagg
     4201 gcgcaggcca gataccagtc ggccggtgcc gaccagatcg cgatcgcgtc accgacaccc
     4261 gatcggttca gccgcagcgg cgccgcgtcg accagccggt cgtcgatgat cagcgcggag
     4321 ggccaggcgg acggcttggt gcgcttggct ccgccgatca ccagcacgct gagcgggtcg
     4381 gagaatccgt tcaccgacgc ggcggtctgc accgcaacca gcggcgcccc cgagcgtgcc
     4441 gataccacct tgcccaggtc ggtgacggtg cccgaaccga gactgacgat cagcgccgga
     4501 cggcccacct cggccaccgt cgactcgatg gtcgcctcgt ccaatctcac ctgcgcacgc
     4561 gtggcccgca cgaccaccat ccgcacctcg ccgaccagtt cgctcaccca ggcgaccagg
     4621 tcgcgtccag cggcggcctt cggcacgtcg tcgaggagca gcacgcgctc ccccgaaggc
     4681 gccagaaggc ggcactggga ggaaagtccg tccagtgccg ccggaccggt ggccaggtgg
     4741 ctcaacgccc cctgcgagac ctcgcgcgcg aagtcgtcca tttgcaccaa tcagccctcg
     4801 cgccgggtga tcgcctgcag cttgtcccag atcggcggca tggcctggcg gtactccttg
     4861 tagatcgcga aatactcgtc gtagagatcg gtcttcttct tcttgggctt gtacttgcgc
     4921 aagatgttga ccgtcgagtc ggcgccctgt tcgtagctgt cgaacatgcc gacgccgacg
     4981 ccggccacta tcgccgcgcc cttggcgccg aactcggtac cggcgggcac gaccaccttg
     5041 cgacccagca catcggcgaa gatctgcgcc cacaccggcg agttggcgcc gccgccggcc
     5101 atccgcacca cggtgacctc ggtcggaatg gcgtcgaggc agtcacggat gccgtaggcg
     5161 acaccctcgt acaccgcgcg cagcaggtcg ttgcgggtgt gcgcgccggt gatgccgatg
     5221 aacgaggccc gggcggacgg cttgacgaac ggtgcccgct cgccgccggg cgcggtgtag
     5281 ccgtggaaca tcacgccacg cgagccgacc ggcacgccct tgaccacctc gtccaggtac
     5341 tcgaagaacg tgtactgagg cccgcgggcc tcggcctcgc gccggaagct gccgccgaac
     5401 tcgcgcaggt accagtcgac gttcggggtg cacgagctca tgcccagggc gcggatccac
     5461 gtggtcgggc tgtgcggaat gacccagccg acgtcgtccg gctcgatcgt gtcgccgaga
     5521 tcctcggtcg cgacagtgac gataccggcg gtgccgatca ccgccatgca gtcgcccgga
     5581 cgggccacgc ccaccccgag cgagctggcg gtgatgtcca tctggccctt gaacaccggg
     5641 gtgccctcgg ccaggccggt caggcccgcg gcctcggcgg tgatcgagcc gatctgcgtg
     5701 atcggggact tgatcaccgg cagcaccgac ttggcctcgg tcagccccag gtgggtgagc
     5761 acctcgtcgg accagtcgcg ggtgcgtacg tcgatgccgc ccagggaggc gtcggacggg
     5821 tcggtggaca gatcgccggt gagcttgtac tcgacccagt ccttgcacca caggttggtg
     5881 gcgccctgaa gaatctcggg ctggttctcg agccgccagc gcagcagcgc cgaggtggtg
     5941 ccggcgtagg gaccgaggcc cgagatctgg aactgcttgc tgatcgtgcc gtcggcgtac
     6001 caggtgtcga tgatgtcggc ggcgcgcccg tcggtccaca gaaaggccgg tccggccggc
     6061 tcgccctcgg cgtcgaccag ccaggtaccg tcaccctggc ccgtggccga gatcgcggcg
     6121 atgtcggacg cgtgcacggc cgaaccctcg atcgcgtcgc ggatcgcgag cgtggcggcc
     6181 acccagacgg tctccatgtc ttcttcggcc atgtaggtgg tcgggttgtc gatcgggacg
     6241 ttgtgggcgc cgcggctgag ctcgttgccg tgctcgtcga agattgcggc cttgatgacg
     6301 ctcgtcccgg cgtcgatacc aagaaggtac tggggcatgg gcaccctttc gaaaacggtg
     6361 acgcgctccg ctgcgcgtac acggtggctg cgatgctaac ccgtcttgta tagacatgtc
     6421 tagtggtgga catacgttct gcggccgtgc taacaaggcg ccatgcgcgc cctggtgaag
     6481 tacgcccccg gccccggcaa cgtcgagctt cgggacgttc cacggccccg tccgggccct
     6541 gggcaggtgc tgatcgaggt ggcgttcgca ggcgtctgcg gcaccgaccg cagcgctctg
     6601 gacgggcact tcgccggcct gatcccccgc accctggggc acgaggcgtc cgggatcatc
     6661 gccgagttgg ggcccgacct gcgacgctcc gacctggccg tcggcgaccg ggttaccttc
     6721 gag
//
LOCUS       DAOSUG010000107         6721 bp    DNA     linear   ENV 10-JUN-2024
DEFINITION  MAG TPA_asm: Micropruina sp. isolate AMR_MDS_2195
            NODE_5473_length_6721_cov_4.022502, whole genome shotgun sequence.
ACCESSION   DAOSUG010000107 DAOSUG010000000
VERSION     DAOSUG010000107.1
DBLINK      BioProject: PRJNA925477
            BioSample: SAMN32864262
            Sequence Read Archive: SRR4236673
KEYWORDS    WGS; ENV; Metagenome Assembled Genome; MAG; Third Party Data; TPA;
            TPA:assembly.
SOURCE      Micropruina sp. (wastewater metagenome)
  ORGANISM  Micropruina sp.
            Bacteria; Bacillati; Actinomycetota; Actinomycetes;
            Propionibacteriales; Nocardioidaceae; Micropruina.
REFERENCE   1  (bases 1 to 6721)
  AUTHORS   Abdulkadir,N., Saraiva,J.P., Zhang,J., Stolte,S., Gillor,O.,
            Harms,H. and Rocha,U.
  TITLE     Genome-centric analyses of 165 metagenomes show that mobile genetic
            elements are crucial for the transmission of antimicrobial
            resistance genes to pathogens in activated sludge and wastewater
  JOURNAL   Microbiol Spectr 12 (3), e0291823 (2024)
   PUBMED   38289113
REFERENCE   2  (bases 1 to 6721)
  AUTHORS   Abdulkadir,N.
  TITLE     Direct Submission
  JOURNAL   Submitted (24-MAR-2023) Environmental Microbiology, Helmholtz
            Center for Environmental Research -UFZ, Permoserstr 15, Leipzig
            04318, Germany
COMMENT     The annotation was added by the NCBI Prokaryotic Genome Annotation
            Pipeline (PGAP). Information about PGAP can be found here:
            https://www.ncbi.nlm.nih.gov/genome/annotation_prok/
            
            ##Genome-Assembly-Data-START##
            Assembly Method        :: SPAdes v. 3.11.1
            Genome Representation  :: Full
            Expected Final Version :: Yes
            Genome Coverage        :: Not Applicable
            Sequencing Technology  :: Illumina MiSeq
            ##Genome-Assembly-Data-END##
            
            ##Genome-Annotation-Data-START##
            Annotation Provider               :: NCBI
            Annotation Date                   :: 05/04/2023 04:57:10
            Annotation Pipeline               :: NCBI Prokaryotic Genome
                                                 Annotation Pipeline (PGAP)
            Annotation Method                 :: Best-placed reference protein
                                                 set; GeneMarkS-2+
            Annotation Software revision      :: 6.5
            Features Annotated                :: Gene; CDS; rRNA; tRNA; ncRNA
            Genes (total)                     :: 2,776
            CDSs (total)                      :: 2,750
            Genes (coding)                    :: 2,714
            CDSs (with protein)               :: 2,714
            Genes (RNA)                       :: 26
            tRNAs                             :: 24
            ncRNAs                            :: 2
            Pseudo Genes (total)              :: 36
            CDSs (without protein)            :: 36
            Pseudo Genes (ambiguous residues) :: 0 of 36
            Pseudo Genes (frameshifted)       :: 2 of 36
            Pseudo Genes (incomplete)         :: 32 of 36
            Pseudo Genes (internal stop)      :: 2 of 36
            ##Genome-Annotation-Data-END##
FEATURES             Location/Qualifiers
     source          1..6721
                     /organism="Micropruina sp."
                     /mol_type="genomic DNA"
                     /submitter_seqid="NODE_5473_length_6721_cov_4.022502"
                     /isolate="AMR_MDS_2195"
                     /isolation_source="Activated sludge of municipal
                     wastewater treatment plants"
                     /db_xref="taxon:2737536"
                     /environmental_sample
                     /geo_loc_name="Canada"
                     /lat_lon="49.25 N 123.1 W"
                     /collection_date="2015-06-16"
                     /metagenome_source="wastewater metagenome"
                     /note="metagenomic"
     gene            26..1687
                     /gene="ctaD"
                     /locus_tag="PLF56_04985"
     CDS             26..1687
                     /gene="ctaD"
                     /locus_tag="PLF56_04985"
                     /EC_number="7.1.1.9"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_008363159.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_function: GO:0004129 - cytochrome-c oxidase activity
                     [Evidence IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="cytochrome c oxidase subunit I"
                     /protein_id="HOQ52950.1"
                     /translation="MQYLHTTDPKVLGVMYIATSFAWFMLGGLMALFMRAELAQPGMQ
                     FLSPEQYNQLFTMHGTIMLLFYATAVVFGFANYVLPLQLGAPDVAFPRLNAFSYWLYF
                     FGSAMACSGFLSPDGAASFGWFAYAPLSNAVHSPGVGGDLWLMGLYLLGLSSILGAVN
                     FITTISTMRMPGMTMFRMPIFTWNIFVTSMMILLAFPVLAAGLLGLEADRRLGTHLFD
                     PSTGGPLLWEHLFWFFGHPEVYIIALPFFGIITEILPVFSRKPVFGYVGLVAATMSIG
                     ALSIAVWAHHMFVTGAVNLPFFSFMTFLIAVPTGVKFFNWIGTMWGGSLTFETPMIWA
                     IGFLTTFLFGGLTGVILASPPLDFHVSDTYFVVAHFHYVLFGTVVFAMFGGFYFWWPK
                     ITGRMLNERWGRVHFWTLFFGFHLTFLVQHWLGIEGMQRRIADYLPSEGFQFLNQVST
                     VGAFLLGVSMLPFFWNIWITRNTPKVTVDDPWGWGRSLEWATSSPPPRHNFTSLPRIR
                     SESPAFDLHHPEIHGVDRTEELDVLLTGSSGNGSQPSSIENDKGE"
     gene            1691..2086
                     /locus_tag="PLF56_04990"
     CDS             1691..2086
                     /locus_tag="PLF56_04990"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_002527710.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="cytochrome c oxidase subunit 4"
                     /protein_id="HOQ52951.1"
                     /translation="MKVEGWMFGAIAIFLAVVSPVYWFMSHEIIGTVALIMSFLLFAM
                     VSVFMFTLGRKMDDRLEDRKDAEIVEGAGAIGFFPPKSIWPFWTALTVQLLALGPVFG
                     WWISILAVCLGIWALSGWVFEFYRGDYQH"
     gene            2497..3339
                     /locus_tag="PLF56_04995"
     CDS             2497..3339
                     /locus_tag="PLF56_04995"
                     /inference="COORDINATES: ab initio
                     prediction:GeneMarkS-2+"
                     /note="Derived by automated computational analysis using
                     gene prediction method: GeneMarkS-2+."
                     /codon_start=1
                     /transl_table=11
                     /product="hypothetical protein"
                     /protein_id="HOQ52952.1"
                     /translation="MLTAALGLSGSVAQADTPVRAPQAPPIVPPPTPKKTTSSARTEA
                     VSSAASYPPAVAELTYVPLAPCVIADSRKGTGGNKSKFGSSTTRTFTVTGTSGFTAQG
                     GQSSGCGVPANAEAVALTAVVIGPSKAGALRIWPSTGAMPSVDYANYGKFTMSNTGTV
                     ALNGPSIKVKNYTSKTNLALRVSGYYLPRIAGLVGGGGQVYSGTGILSVTNPSPGRFE
                     VTVDRDVTYCTPMVNPYNAGPGVYANAYAFANNKVSVFTWYLDGITHNEAPLTMYVYL
                     TVEC"
     gene            3403..4194
                     /locus_tag="PLF56_05000"
     CDS             3403..4194
                     /locus_tag="PLF56_05000"
                     /inference="COORDINATES: protein motif:HMM:NF026098.2"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="pyrroline-5-carboxylate reductase dimerization
                     domain-containing protein"
                     /protein_id="HOQ52953.1"
                     /translation="MGSTRSTSGTGSEHEARLTVLGVGSIAEAIVTGLSASDPERRVV
                     LSPRNPARSARLAAAHPNVSVASSNQAALDAADTVLLCLRAADAPVLAELSFRPEQRL
                     ITVMPSPGVNDLAALVGRVAAISRAIPAVAVADRAGLTPVYPAGSAAQTLFDELGGSM
                     ALASEQQLDATSAASATVATYFALLQAIAEWLAGRGVDERQARRLVAAVFAGASAELA
                     HSSDFAELAAQHATPGGQNERLVARLRAAGMFGQLTDGLDALLAE"
     gene            complement(4204..5646)
                     /locus_tag="PLF56_05005"
     CDS             complement(4204..5646)
                     /locus_tag="PLF56_05005"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_014847076.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_function: GO:0003938 - IMP dehydrogenase activity
                     [Evidence IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="GuaB1 family IMP dehydrogenase-related protein"
                     /protein_id="HOQ52954.1"
                     /translation="MRFLNPKPGYDLTYDDVFMTPRRSSVTSRLDVDLTSTDGLRLPL
                     PLVVANMTAISGRRMAETVARRGGVAIIPQDLPTGIVAQTIGRVKAAHTVFDTPVHVT
                     PHTTIGETLNLLPKRAHGLVVVVEDGAPIGLLGPSEAAGLDRFVQVHEAMSTDLTIVD
                     PDTSPAEVFELLSTRRHRVALAVRDGRLTGLMTAKGALRATLYAPAVDAEGRLRVGAA
                     VGISKDVERRASDLVDAGADVLVMDTAHGHQERMIEGLTRVRAMLNARGSTVPVVAGN
                     VVTAEGTADLIEAGADVVKVGVGPGAMCTTRMQTGVGRPQFSSVLDCAAEAHEAGKAI
                     WADGGVRHPRDVALALAAGAGAVMIGSWFAGTHESAGDLVTDHDGRTYKESFGMASAR
                     AVRARTREQSRYERARAALFEEGISQSRMYLDPERPGVEDLIDFIVSGVRSSCTYAGA
                     RSLAEFAQNAVVGVQSASGFNEGRPLHVSW"
     gene            complement(6040..6721)
                     /locus_tag="PLF56_05010"
                     /pseudo
     CDS             complement(6040..6721)
                     /locus_tag="PLF56_05010"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_002513974.1"
                     /note="incomplete; partial in the middle of a contig;
                     missing N-terminus and C-terminus; Derived by automated
                     computational analysis using gene prediction method:
                     Protein Homology."
                     /pseudo
                     /codon_start=2
                     /transl_table=11
                     /product="ubiquinol-cytochrome c reductase cytochrome b
                     subunit"
BASE COUNT          993 a         2309 c         2237 g         1182 t
ORIGIN      
        1 gcgggccccg cgtggggcga agttcctgca gtacctgcac accaccgacc ccaaggtgct
       61 cggggtcatg tacatcgcca cctcgttcgc ctggttcatg ctcggtggcc tgatggccct
      121 gttcatgcgg gccgagctgg cgcagccggg catgcagttc ctctcgcccg agcagtacaa
      181 ccagctcttc accatgcacg gcacgatcat gctgctgttc tatgcgacgg ccgtggtgtt
      241 cgggttcgcc aattacgtgc tgcccctgca gctcggcgcc cccgacgtgg cgttcccccg
      301 gctgaacgcc ttctcgtact ggctgtactt cttcggctcg gccatggcct gctcgggctt
      361 cctgagccct gacggtgcgg cgtccttcgg ctggttcgct tatgcgccgc tgtcgaacgc
      421 ggtgcacagc cctggcgtgg gcggcgatct gtggctgatg ggcctgtacc tgctcggcct
      481 ctcgtcgatc ctcggcgcgg tcaacttcat cacgaccatc tcgaccatgc gcatgcccgg
      541 catgaccatg ttccggatgc ccatcttcac ctggaacatc ttcgtgacct cgatgatgat
      601 cctgctggcc ttccccgtgc tggccgctgg cctgctgggc cttgaggccg accgccggct
      661 cggcacccac ctgttcgatc cgtccacggg cgggccgctg ctgtgggagc acctgttctg
      721 gttcttcggg caccctgagg tctacatcat cgcgctgccg ttcttcggca tcatcaccga
      781 gatccttccg gtgttcagtc gcaagccggt gttcggctac gtcggcctgg tcgccgccac
      841 gatgtcgatc ggcgccctga gcatcgcggt gtgggcgcac cacatgttcg tcaccggtgc
      901 ggtgaacctg ccgttcttct cgttcatgac gttcctgatc gccgtgccga cgggggtgaa
      961 gttcttcaac tggatcggca ccatgtgggg cggttccttg accttcgaga cccccatgat
     1021 ctgggcgatc ggattcttga ccaccttctt gttcggtggt ctgaccggcg tcatcctggc
     1081 cagcccgccg ctcgactttc acgtctcgga cacctacttc gtggtggcgc acttccacta
     1141 cgtgctgttc ggcaccgtgg tgttcgccat gttcggcggc ttctacttct ggtggcccaa
     1201 gatcaccggg cgcatgctga acgaacgctg gggccgggtg cacttctgga ccctgttctt
     1261 cggcttccac ctgaccttcc tggtgcagca ctggctgggc atcgagggca tgcagcggcg
     1321 catcgccgac tacctgccgt ccgagggctt ccagttcctc aaccaggtct cgaccgtcgg
     1381 cgcgttcctg ctgggcgtct cgatgttgcc gttcttctgg aacatctgga tcacccgcaa
     1441 cacgcccaag gtgacggtgg acgatccgtg gggctggggg cgctcgctgg agtgggccac
     1501 gtccagcccg ccgccgcggc acaacttcac cagcctgcct cgcatccgct ccgagtcgcc
     1561 tgcttttgac ctgcaccatc ccgagattca tggggtggat cgcaccgaag aactggacgt
     1621 gctgttgacc ggctcgtcgg gcaacggcag tcagccgtcc tcgatcgaga acgacaaggg
     1681 ggagtagacc atgaaggtcg aaggctggat gttcggcgcg atcgcgatct ttctcgccgt
     1741 cgtgtcaccg gtgtactggt tcatgtcgca cgagatcatc ggcacggtcg cgctgatcat
     1801 gtcgttcctg ctgttcgcca tggtctcggt gttcatgttc accctcggcc gcaagatgga
     1861 cgaccggctc gaagaccgca aagacgccga gatcgtcgaa ggcgcaggtg cgatcggctt
     1921 ctttccgccc aagagcatct ggcccttctg gacagccctc accgtgcagt tgctcgccct
     1981 cggcccggtg ttcggctggt ggatcagcat tcttgcggtc tgcctcggta tttgggctct
     2041 gagcggctgg gttttcgagt tctaccgggg cgattaccag cactgagcgg tacctgctga
     2101 acgagatcgc agacaagctg cgcggattga tgcgtccgac ttcggtgaac cgtgcttgag
     2161 cgggtgggtt ttgctaccgc agtcggtagc aaatcccacc cgtagtctcg atccgccgat
     2221 ggacgccatc gcggcgtccc aggaaactgc ggatccatgc gcgatcgttg gttgaggtaa
     2281 ccgaaaccgc tgctgcaacg gcgtctcgac agctccaccg gcgatggact ggcgtgcctg
     2341 gacggccgtg gatcacgggc gaggcctggg caatgttacg cattgctcgg atcacccgcc
     2401 aagcttggat ggcgcctgac ccggcatcct gccggcgtca cacagtcggg ggccaccatg
     2461 ttcgtcaggc gtctgtccat cgttctcgct tcgctcatgc tcacggcggc gctgggcctc
     2521 agcggctccg tcgcgcaggc cgacacccct gtccgggcac cgcaggcgcc gcccatcgtt
     2581 cctccgccga cgcccaagaa gacgacgtcg agcgcccgta ccgaggccgt gtccagcgcg
     2641 gcgtcctacc caccggccgt cgctgagctg acctacgtgc cgctcgcgcc gtgcgtgatc
     2701 gccgacagcc gcaagggcac cggcggcaac aagagcaagt tcggctccag caccactcgc
     2761 acgttcaccg tcaccggcac ctcgggcttc accgcccagg gtgggcagtc gagcggttgc
     2821 ggggttccgg ccaatgccga ggcggtggcc ctcaccgcgg tggtgatcgg gccgtccaag
     2881 gccggggcgc tacgcatctg gccgtccacg ggggccatgc cgtcggtcga ctacgcgaac
     2941 tacggcaagt tcaccatgtc gaacaccggc accgtggcac tcaatggacc ctccatcaag
     3001 gtgaagaact acacctcgaa gacgaatctc gccctgcggg tctcgggcta ctacctgccc
     3061 cggatcgccg gactggtcgg gggcggcggg caggtgtaca gcggcaccgg catcctgtcg
     3121 gtgaccaacc cgagccctgg caggttcgag gtgacggtcg accgcgacgt gacgtactgc
     3181 acaccgatgg tgaaccccta caacgccggc ccgggcgtgt acgccaacgc gtatgccttc
     3241 gcgaacaaca aggtgagcgt cttcacctgg tacctggacg gcatcacaca caacgaggcg
     3301 cccttgacca tgtatgtgta cctgactgtg gagtgctgac ggatctgggt tcgaccctcg
     3361 gccggcgccg ctccgcaggg cgtcgcgcgc cacaatggcg ccatgggatc gacacgctcg
     3421 accagcggta cgggaagcga acatgaggcg cggctcaccg tgctgggcgt ggggtcgatc
     3481 gccgaggcga tcgtcaccgg actgagtgcg tccgacccgg agcggcgcgt cgtgctctca
     3541 ccgcgcaacc ccgcgcggtc ggcccggctc gcggcggcac acccgaacgt gtcggtggcg
     3601 agcagcaacc aggcggcgct cgacgccgcc gacaccgtgc tgctctgcct gcgcgcagcc
     3661 gacgcaccgg tgctggcgga gttgtcgttt cggcccgagc agcgcctgat caccgtgatg
     3721 ccctcgccgg gcgtgaatga cctggccgcc ctggtcggca gggtcgccgc gatctcgcgg
     3781 gcgatcccgg cggtcgcagt ggccgaccgg gccgggctca ccccggtcta tccagccggc
     3841 agcgctgcgc agacgctgtt cgacgagttg ggcggctcga tggcgctggc gagcgagcag
     3901 caactggatg ccacttcggc ggccagtgcc acggtcgcga cctacttcgc cttgctgcag
     3961 gccatcgccg agtggctggc cggacggggc gtggatgagc gccaggcgcg ccgactggtc
     4021 gccgcggtgt tcgccggggc gtcggcggaa ctggcgcact cgtccgactt cgctgaactc
     4081 gccgcgcagc atgccacgcc gggcgggcag aacgagcgac tggtcgcgcg gctgcgggcg
     4141 gccggcatgt tcggtcagct caccgacggt ctggacgccc tgctggccga gtgaccgtgc
     4201 ggctcaccag ctgacgtgca gggggcgtcc ctcgttgaag ccggacgccg actgcacgcc
     4261 caccacggcg ttctgggcga attcggccag gctgcgcgcc cccgcgtagg tgcaggacga
     4321 tcgcacgccg gacacgatga agtcgatcag gtcctccacc cccggacgct ccgggtcgag
     4381 atacatgcgc gattgcgaga tgccctcttc gaagagcgca gcccgggccc gttcgtagcg
     4441 cgactgctcg cgggtgcggg ctcgaaccgc ccgtgcggac gccatgccga acgactcctt
     4501 gtaggtgcgt ccgtcgtggt cggtgaccag atcaccggcc gactcatgcg tgcctgcgaa
     4561 ccacgacccg atcatcaccg ccccggcgcc ggcggcgagc gccaaggcca cgtcccgcgg
     4621 gtgccggacg ccgccgtccg cccagatcgc cttgccggcc tcgtgggctt cggcggcgca
     4681 gtcgagtacc gagctgaact gcggacgacc cacgccggtc tgcatgcgtg tggtgcacat
     4741 ggcgccggga cccacgccca ccttcaccac gtcggcaccg gcctcgatca ggtccgcggt
     4801 gccttcggcg gtcaccacgt tgcccgccac caccggcacg gtgctgccac gggcgttcag
     4861 catggcccgc acccgcgtca gtccttcgat catgcgctcc tgatggccgt gggcggtgtc
     4921 catcaccagc acgtccgcgc cggcgtccac caggtcggac gcgcggcgct ccacgtcttt
     4981 gctgatgccc acggcggcac cgacccggag cctgccctcg gcgtccaccg ccggcgcgta
     5041 cagggtggcg cgcagcgcac ccttggcggt catcagcccc gtcaagcggc cgtcgcgtac
     5101 cgccagcgcc acccggtgcc gccgggtgct cagcaactcg aacacttcgg cgggggaggt
     5161 gtcgggatcg acgatcgtca gatcggtcga catggcctcg tgaacctgca cgaaccggtc
     5221 caggccggcc gcctcggacg ggccgagcag accgatcggg gcaccgtcct cgaccaccac
     5281 gaccaggccg tgcgcccgct tgggtagcag gttcagcgtc tcaccgatcg tggtgtgcgg
     5341 cgtgacgtgc accggcgtgt cgaacaccgt gtgggccgcc ttcacccggc cgatggtctg
     5401 cgcgacgatg ccggtgggca ggtcctgcgg aatgatcgcc accccgccgc gccgggccac
     5461 cgtctcggcc atccgtcggc ccgagatggc cgtcatgttg gcgaccacca gcggcaacgg
     5521 caggcgcagg ccgtccgtgc tggtcaggtc gacgtcgagt cgcgaggtca ccgacgagcg
     5581 gcgtggcgtc atgaagacgt cgtcgtaggt gaggtcgtaa ccgggtttgg ggttcagaaa
     5641 acgcacggac ggatcgtacc gggcccggcg ccaggctccg aaccggccgc ggaccccggc
     5701 gcggtacgac cggacgcagc agtgccgccg aacccggtcg gcggcactgt tgctgtggtt
     5761 gctgcggggt cagtgggtgc tacgaccggc gtcgtgcgtg ccggtcagtt cggggccgtc
     5821 gctctcgagt tcgtgcccct caccgccgtg gccgttgacc tggtgaccat cggcgtggtg
     5881 gtcggcttcg gcgatctcgg cagcggtggg cttcgcgatc accccggcgt ggaaccagcg
     5941 cgacatcagg gcccgcagct tgctgacccc ggccccctct tcgagcacgc cgtgtgcgtc
     6001 ggtgtcggga gaggactgca gggccgcgcc ctgcgggtgc tgggtcaggg tgaacttctc
     6061 gtcgatgctg atcggcttgt gcgcctcgga gtagccaccg tcgggggaac gctcgatgac
     6121 gccggactcg ctaccgtgca gcacccgctc ggcatccacg cgctgcagcg agatgcagat
     6181 gcgcttggtg atgatgaacg cgatcacagg agccacgaac accgccaccc gcatgaactc
     6241 ggtcacggcg ttgagcgaca aatggaactg ggtggcgaga atgtcgttgc cgccggccag
     6301 ccagaacatc gcgtagcagg tcatgcccgc gaccccgaga gcggtgcggg tgggcttgtt
     6361 gcgcggccgg tccagcacat ggtgttcggc ggtgtcgccg gtgatccagc gctcgatgaa
     6421 ggggtaggcc cccaacagga cgaacagcgc tgtcatcagc cccacgccgg gcaggaacac
     6481 gttccacgac caggtggtgc tgcccagatg ccattccacg tccggcatga tgcggatggc
     6541 gccctcgacc cagcccatgt accagtcggg ctgcgagccg gcggtgacct tcgccgggtc
     6601 gtaggggccg tacgtccaca cggggttgat ctggaagagg ccgcccatga gcaccaggaa
     6661 gccgaacacg atgaagaaga agccgccggc cttggccatg tacacgggga acatcgggta
     6721 c
//
LOCUS       DAOSUG010000108         6720 bp    DNA     linear   ENV 10-JUN-2024
DEFINITION  MAG TPA_asm: Micropruina sp. isolate AMR_MDS_2195
            NODE_5474_length_6720_cov_3.866167, whole genome shotgun sequence.
ACCESSION   DAOSUG010000108 DAOSUG010000000
VERSION     DAOSUG010000108.1
DBLINK      BioProject: PRJNA925477
            BioSample: SAMN32864262
            Sequence Read Archive: SRR4236673
KEYWORDS    WGS; ENV; Metagenome Assembled Genome; MAG; Third Party Data; TPA;
            TPA:assembly.
SOURCE      Micropruina sp. (wastewater metagenome)
  ORGANISM  Micropruina sp.
            Bacteria; Bacillati; Actinomycetota; Actinomycetes;
            Propionibacteriales; Nocardioidaceae; Micropruina.
REFERENCE   1  (bases 1 to 6720)
  AUTHORS   Abdulkadir,N., Saraiva,J.P., Zhang,J., Stolte,S., Gillor,O.,
            Harms,H. and Rocha,U.
  TITLE     Genome-centric analyses of 165 metagenomes show that mobile genetic
            elements are crucial for the transmission of antimicrobial
            resistance genes to pathogens in activated sludge and wastewater
  JOURNAL   Microbiol Spectr 12 (3), e0291823 (2024)
   PUBMED   38289113
REFERENCE   2  (bases 1 to 6720)
  AUTHORS   Abdulkadir,N.
  TITLE     Direct Submission
  JOURNAL   Submitted (24-MAR-2023) Environmental Microbiology, Helmholtz
            Center for Environmental Research -UFZ, Permoserstr 15, Leipzig
            04318, Germany
COMMENT     The annotation was added by the NCBI Prokaryotic Genome Annotation
            Pipeline (PGAP). Information about PGAP can be found here:
            https://www.ncbi.nlm.nih.gov/genome/annotation_prok/
            
            ##Genome-Assembly-Data-START##
            Assembly Method        :: SPAdes v. 3.11.1
            Genome Representation  :: Full
            Expected Final Version :: Yes
            Genome Coverage        :: Not Applicable
            Sequencing Technology  :: Illumina MiSeq
            ##Genome-Assembly-Data-END##
            
            ##Genome-Annotation-Data-START##
            Annotation Provider               :: NCBI
            Annotation Date                   :: 05/04/2023 04:57:10
            Annotation Pipeline               :: NCBI Prokaryotic Genome
                                                 Annotation Pipeline (PGAP)
            Annotation Method                 :: Best-placed reference protein
                                                 set; GeneMarkS-2+
            Annotation Software revision      :: 6.5
            Features Annotated                :: Gene; CDS; rRNA; tRNA; ncRNA
            Genes (total)                     :: 2,776
            CDSs (total)                      :: 2,750
            Genes (coding)                    :: 2,714
            CDSs (with protein)               :: 2,714
            Genes (RNA)                       :: 26
            tRNAs                             :: 24
            ncRNAs                            :: 2
            Pseudo Genes (total)              :: 36
            CDSs (without protein)            :: 36
            Pseudo Genes (ambiguous residues) :: 0 of 36
            Pseudo Genes (frameshifted)       :: 2 of 36
            Pseudo Genes (incomplete)         :: 32 of 36
            Pseudo Genes (internal stop)      :: 2 of 36
            ##Genome-Annotation-Data-END##
FEATURES             Location/Qualifiers
     source          1..6720
                     /organism="Micropruina sp."
                     /mol_type="genomic DNA"
                     /submitter_seqid="NODE_5474_length_6720_cov_3.866167"
                     /isolate="AMR_MDS_2195"
                     /isolation_source="Activated sludge of municipal
                     wastewater treatment plants"
                     /db_xref="taxon:2737536"
                     /environmental_sample
                     /geo_loc_name="Canada"
                     /lat_lon="49.25 N 123.1 W"
                     /collection_date="2015-06-16"
                     /metagenome_source="wastewater metagenome"
                     /note="metagenomic"
     gene            <1..368
                     /locus_tag="PLF56_05015"
     CDS             <1..368
                     /locus_tag="PLF56_05015"
                     /inference="COORDINATES: protein motif:HMM:NF013866.2"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_function: GO:0016787 - hydrolase activity [Evidence
                     IEA]"
                     /codon_start=3
                     /transl_table=11
                     /product="alpha/beta hydrolase"
                     /protein_id="HOQ52955.1"
                     /translation="VIGGHSLGGAMAADYAARHADQLSGLILLAAYPPDGVDLTHTRF
                     GSLSLLAEHDQVAAADKVRGGLAHLSPSSRLEVIPGAVHAFFGRYGPQQGDGVPTVRR
                     EQAEQAIIERIVDYLSPIA"
     gene            complement(922..2640)
                     /locus_tag="PLF56_05020"
     CDS             complement(922..2640)
                     /locus_tag="PLF56_05020"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_012921077.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="AMP-binding protein"
                     /protein_id="HOQ52956.1"
                     /translation="MPYTGRPATPGTDAVRAARDFLFAHSTDYDGAVAGFRWPQLTEF
                     NFGLEWFDVVAGEHPDRPAVAIVDAELNHVSTSYGELAEASDRVANWLRQLGMRRGDA
                     MIVMLNNTIELWQVLLGLLKVGGVAIPTSTLLSDVELHYRVERAAARFTIAPAELAAR
                     FSGLPAEVTRIGVGAAVPGWVGFADSSGAAATFTPDGPTPADDTCLLYFTSGTTARPK
                     LVRHTQVSYPVGHLSTMWWLGVREGDVHLNISSPGWGKHAWSNFFSPFLAQATVFIFN
                     YARFDAGALMRIMDTHQVTTFCAPPTVWRMLIQADLTQLSTPPRELVGAGEPLNPEVI
                     GQVRTAWGSTIRDGFGQTETTCWIGNSPGQRVKDGSMGRPMPGYTAVLLDPATGEPSP
                     DEGEICLDLSNPPLALMGGYWREPDYTAEAMRGGYYHSGDIASRDGDGYLTYVGRADD
                     VFKASDYKISPFELESVLLQHPAVAEAAVVPSPHPVRAAVPKAYLLLAEAHQADAEVA
                     RSIFAHARANLSGFQHVRIIEFVNELPKTISGKIRRIELRSREAARVAGDDTDAGEQF
                     HWRDHR"
     gene            complement(2795..3781)
                     /locus_tag="PLF56_05025"
     CDS             complement(2795..3781)
                     /locus_tag="PLF56_05025"
                     /EC_number="1.1.1.37"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_015071129.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_function: GO:0016615 - malate dehydrogenase activity
                     [Evidence IEA];
                     GO_process: GO:0006108 - malate metabolic process
                     [Evidence IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="malate dehydrogenase"
                     /protein_id="HOQ52957.1"
                     /translation="MSTPVKVAVTGAAGQICYSLLFRIASGALLGPDQQVELRLLEIT
                     PALKALEGVVMELDDCAFPTLAKVEIGDDPTKVFDGVNLAMLVGARPRTAGMERGDLL
                     SANGAIFTAQGKALNDVAADDIKVLVTGNPANTNALIAMKNAPDIPTERFNALTRLDH
                     NRAISQLAAKVGVPVTEIKKMTIWGNHSATQYPDLFHAEVNGQNAATLVDDQAWIESD
                     FIPTVAKRGAAIIAARGSSSAASAANATIEHMRDLVKGSPAGDWVSMAVPSDGSYGVP
                     EGIISSFPCTTSDGTWSIVQGLEIDDFSRAKIDASVAELQDEAKAVTELGLI"
     gene            4113..>6720
                     /locus_tag="PLF56_05030"
     CDS             4113..>6720
                     /locus_tag="PLF56_05030"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_012920396.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_function: GO:0003824 - catalytic activity [Evidence
                     IEA];
                     GO_process: GO:0005975 - carbohydrate metabolic process
                     [Evidence IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="alpha-amylase family glycosyl hydrolase"
                     /protein_id="HOQ52958.1"
                     /translation="MQRRKSLALIMILALMMGWFTSSAPARAAERTVTLVGDLQSELG
                     CSADWQPTCTATDLTQKGTSYSRTFTIPAGSWQLKVAINHSWDESHGADGGSANISLA
                     LTTATKLLISYDDTSHLVTIKPVDLPGSTSTADRRLAVGSLREALTKERFYFLMADRF
                     ANGDKANDAGGLTGGRLKTGFDPTDSGFYHGGDLKGVTQKLDYIKGLGTTAIWLTPSF
                     KNNPVQGSGDNVSAGYHGYWITDFTQIDPHLGTNADMKNLIAKAHAKGMKVFFDIITN
                     HTADIIDYTNPGTGTDAYKYIDKETSPYKTAAGEKFDDRDYAGKNTFPELDAATSFPY
                     TPTFRSEADKTVKVPAWLNDPTLYHNRGDSTFAGESSTYGDFVGLDDLFTENHTVVDG
                     FEKIYQTWADFGIDGFRIDTVKHVNTEFWQQFIPSVLDHAKAKGNDDFFMFGEVYDSS
                     PTFTSQYTTKAKLQATLDFGFQNAALGFAQGKATTGLRDVYAGDDWYTDADSNAYQLP
                     TFLGNHDMGRLAYLLRSSGVKASDTLTRLKLANSLMYLTRGQPVVYYGDEQGFAGSGG
                     DKASRQDMFASKVKSYNDEEVIGGASGSKDRYNTNATLYRQIAQLAKLRAAHPALADG
                     AQIARYSSNEAGIFAVSRIDKAAKREYLVVTNNAATSKTATFATYSPSIRFDSLLGGG
                     SGVRADRFGRVTVTVPAYGTTVLRATGRMPAASAAPVILPLAPKSGGTVGGRAEISAA
                     LADTSAFAEVSFYYRPAGTSDWKLIGTDDNPRYRVYQDVSGMPKGSLLEYRVVAKDLQ
                     GRYSATSTYGFVGDAGGTTGPSTISYDPPEKQPDSVSVPGSHNASMGCTGGPNGDGNW
                     FPVCPQAQLTLDA"
BASE COUNT         1156 a         2213 c         2279 g         1072 t
ORIGIN      
        1 tggtgatcgg cgggcattcg ctgggcggcg cgatggcggc cgactacgca gcccggcacg
       61 ccgaccagct cagcggactg attctgctgg ccgcgtaccc gcccgacggg gtcgacctga
      121 cgcacacgcg cttcggcagc ctgtcgctgc tcgccgagca cgatcaggtc gccgccgcgg
      181 acaaggtgcg gggcggactg gcgcacctgt cgccctccag caggctcgag gtgattccgg
      241 gggcggtgca cgccttcttc ggacgctacg ggccccagca gggcgacggc gtgccgacgg
      301 tgcgccgcga gcaggccgag caggcgatca tcgaacgcat cgtggactac ctgtcgccga
      361 tcgcctgacg gagcgaacgg acgggcccgc tgacgccgcc ctcacctttg cggggctggt
      421 cgcgggtgcg ccaccgggct aagcctggat ccaccgatgg gcgccgtcgc gagcggcacc
      481 agacgggtgg cctggcaagc cggtccgcgc gacggcatac cgggcgtctg gtgagcgtgg
      541 ccgacgaagc cagggtgccc ggatggggcc gcgcagtagg cgaagatcgg tggatccagg
      601 ctaaggtctg aatgctcgat aatcggaggg gtggcctagt aactcgaact gcttgttaaa
      661 gctgtgaata gttgagttca ggagtctagg tgcctagcgg gtatttgtgt ccgcattagt
      721 tccagctccg cttcgagcct tctgatctga atcaggagtt gtctacgtcc ttcagttgga
      781 gcgtcgatgc gtgcggactg tactttcaac ggtgtacctc cctgatcgaa gttgcaccgt
      841 tgaaagtaca gtccccgctg taggcatcca ccaccgacaa gtaaacccaa cgcagcccga
      901 tgccgcccaa tgggacgcct cctaccggtg gtcgcgccag tggaactgtt cgccggcgtc
      961 cgtgtcgtca ccggccaccc gggccgcctc gcggctgcgc agctcgatgc gccggatctt
     1021 tcccgaaatg gtcttgggca actcgttgac gaactcgatt atgcgaacgt gctgaaagcc
     1081 ggaaaggttc gcgcgggcgt gcgcgaagat cgaccgcgcc acctcggcgt ccgcctgatg
     1141 ggcctcggcc agtagcaggt aggccttggg caccgccgct cggacggggt gcgggctggg
     1201 caccacggcg gcctcggcga ccgcgggatg ctgcaacagc accgactcga gttcgaatgg
     1261 gctgatcttg tagtcggacg ccttgaacac gtcgtcggcc cgtccgacgt aggtgaggta
     1321 gccgtcgcca tcgcggctgg cgatgtcgcc ggagtggtag tagccgcccc gcatcgcctc
     1381 ggcggtgtag tcgggctcac gccagtaccc gcccatcagg gccagcgggg ggttcgacag
     1441 gtcgaggcag atctcgccct cgtcggggct cggctcgccg gtggccgggt cgagcagcac
     1501 cgcggtgtag ccgggcatgg gacggcccat ggagccgtcc ttcacgcgct gaccagggga
     1561 gttgccgatc cagcaggtgg tctcggtctg tccgaagccg tcgcggatcg tggaacccca
     1621 ggccgtccgc acctggccga tcacctcggg gttgaggggt tcgccggcgc cgaccagttc
     1681 gcgcggcggc gtgctcaact gcgtcaggtc ggcctggatc agcatgcgcc acaccgtcgg
     1741 gggagcgcag aaggtggtca cctgatgggt gtccatgatg cgcatcagcg cgcccgcgtc
     1801 gaaacgggcg tagttgaaga tgaatacggt ggcctgtgcc aggaacggcg agaagaagtt
     1861 gctccaggcg tgcttgcccc accccggcga actgatgttg aggtgcacgt cgccctcgcg
     1921 cacccctagc caccacatcg tcgacaggtg gccgaccggg tagctgacct gggtgtgccg
     1981 caccagcttg ggccgggccg tggtgcccga ggtgaagtac aacaggcagg tgtcgtcggc
     2041 gggcgtgggg ccgtccgggg tgaaggtggc cgcggccccc gacgaatcgg cgaagcccac
     2101 ccaccccggc acggccgcac cgacgccgat gcgggtcacc tcggcaggca ggccgctgaa
     2161 ccgggcggcg agttcggccg gggcgatcgt gaagcgggcc gcggcccgct cgacgcggta
     2221 gtgcagttcg acgtccgaca gcagggtcga ggtcggaatc gcgacgcctc cgaccttgag
     2281 caggccgagc agcacctgcc acagctcgat ggtgttgttg agcatcacga tcatcgcgtc
     2341 gccgcggcgc atgcccaact ggcgcagcca gttggcgacc cggtcggagg cttcggccag
     2401 ttcgccgtag ctggtgctga cgtgattgag ctcagcgtcg acgatggcga ccgccggccg
     2461 gtcgggatgc tcaccggcga cgacgtcgaa ccattcgagg ccgaagttga actcggtcag
     2521 ctgcggccaa cgaaagccgg cgacggcgcc gtcgtagtcg gtggagtgag cgaagagaaa
     2581 gtcgcgcgcg gctcggacgg cgtcggtgcc gggggtcgcg ggacgtccgg tgtagggcat
     2641 gatgccatca aaccaccacg atgccccctg cgggggcagc ggtcagttcg gtgggaatgg
     2701 tagggaaact ggtgatcgca gcctggtgat gccaggtaga gccggtcgac gccgtacgga
     2761 cggcggtgtc gaccggctca ccctggaagc tggctcagat caggcccagt tcggtgacgg
     2821 ccttggcctc gtcttgcaac tcggcgaccg aagcgtcgat cttggcgcga ctgaagtcgt
     2881 cgatctcgag gccctggacg atgctccacg tgccgtccga ggtggtgcag gggaacgagc
     2941 tgatgatgcc ctcgggcacg ccgtacgagc cgtcggacgg caccgccatc gacacccagt
     3001 cgccggccgg cgagcccttg accaggtcgc gcatgtgctc gatcgtggcg ttggccgccg
     3061 acgcggccga cgaggagccg cgggccgcga tgatcgccgc gccgcgcttg gcgacggtgg
     3121 gaatgaagtc gctctcgatc caggcctggt catcgaccag ggtggcggcg ttctgcccgt
     3181 tcacctcggc gtggaacagg tcggggtact gggtggcgga gtggttgccc cagatggtca
     3241 tcttcttgat ctcggtgacc ggtacgccga ccttggcggc cagctggctg atcgcacggt
     3301 tgtggtcgag ccgggtcagc gcgttgaacc gctcggtggg aatgtcgggc gcgttcttca
     3361 tggcgatcag ggcgttggtg ttggccggat tgccggtgac cagcaccttg atgtcatcgg
     3421 ccgcaacgtc gttgagcgcc ttgccctgag cggtgaagat ggcgccgttg gcgctcagca
     3481 ggtcaccgcg ctccatgccc gcggtgcggg ggcgggcacc gaccagcatg gccaggttga
     3541 ccccgtcgaa caccttggtc gggtcgtcgc cgatctcgac cttggccagc gtggggaacg
     3601 cgcagtcgtc cagctccatc acgacgcctt cgagcgcttt gagtgcgggg gtgatttcga
     3661 gcagtcgcag ttcgacctgc tggtcggggc ccagcagggc gccgctggca atgcggaaca
     3721 gcaggctgta gcagatctgg ccggcggcgc cggtaacggc caccttgacg ggtgtgctca
     3781 cggatgtctc ctcaacgacg agtgctaaga aacttcacgc tatcagcgcc gcctgacgcg
     3841 cgcgggcgat caccggggtt ggggtagccg ctcgtgccgc tcgtcggcca gtgatctcga
     3901 gatcgctgcc cgatggtcgg aagacccgtc cggtttcggg ttgcaacccg aaaccgacaa
     3961 gcgatcccga aactgagcgg cgatctcgag atcgcgggtc ggcgtccagg caggcgagac
     4021 ccgacccggt cggcggacgt tcgccgcagg gtctggttct tgtcggagca gcccgctagg
     4081 ttgccatcgg aacacatcga agaggagccg tcgtgcagcg tcgcaagagt ctcgccctga
     4141 tcatgattct cgccctgatg atgggttggt tcacctcgag cgcgcccgca cgtgctgcgg
     4201 aacgcaccgt caccctggtc ggcgacctac agagcgaact gggctgcagt gccgattggc
     4261 agcccacgtg caccgcgacc gacctcacgc agaagggcac gtcctacagt cgcaccttca
     4321 cgattccggc cggctcgtgg cagctcaagg tggcgatcaa tcattcctgg gacgaaagcc
     4381 acggcgccga cggcggctcg gcgaacattt ccttggcgct gaccaccgcc accaagctgc
     4441 tgatcagcta tgacgacacc tcgcatctgg tcaccatcaa gccggtcgac ctgccgggtt
     4501 cgacgagcac cgccgatcgt cgcttggcgg tcgggtcgct gcgcgaggca ctgaccaagg
     4561 agcggttcta ctttctgatg gccgaccggt tcgccaacgg cgacaaggcc aacgacgcgg
     4621 gaggcctgac cggcggccgc ttgaagaccg gcttcgatcc cacggactcg ggcttctacc
     4681 atggcggcga cctcaagggc gtcacccaga agctggacta catcaaaggc ctgggcacca
     4741 ccgcgatctg gctgaccccg tcgttcaaga acaacccggt gcagggctcc ggcgacaacg
     4801 tcagcgcggg ctaccacggc tactggatca ctgatttcac ccagatcgac cctcacctgg
     4861 gcaccaacgc cgacatgaag aacctgatcg ccaaggcgca tgccaagggc atgaaagtgt
     4921 tcttcgacat catcaccaac cacaccgccg acatcatcga ctacaccaac ccgggcacgg
     4981 gcacggacgc ctacaagtac atcgacaagg agacgtcgcc ctacaagacg gccgccggcg
     5041 agaagttcga cgatcgcgac tacgcaggca agaacacctt ccctgagctg gacgcggcca
     5101 cgtccttccc ctacacgccg acgttccgca gcgaggccga caagaccgtc aaggtgccgg
     5161 cctggctgaa cgacccgacg ctgtaccaca accgcggcga ctcgacgttc gccggcgagt
     5221 cgtccaccta tggcgacttc gtcgggctgg acgacctgtt caccgagaac cacaccgtgg
     5281 tcgacggatt cgagaagatc taccagacgt gggccgactt cggcatcgac gggttccgca
     5341 tcgacacggt caagcacgtg aacaccgagt tctggcagca gttcatcccg tccgtgctcg
     5401 accacgccaa ggccaagggc aacgacgact tcttcatgtt cggtgaggtg tacgacagct
     5461 cgcccacctt caccagccag tacacgacca aggccaagct gcaggccacc ctcgacttcg
     5521 gctttcagaa cgccgcactc gggttcgcgc agggcaaggc aaccacgggc ctgcgcgacg
     5581 tgtacgccgg tgatgactgg tacaccgacg ccgacagcaa cgcctaccag ctgcccacct
     5641 ttctgggcaa tcacgacatg ggccggttgg cctacctgct gcgcagttcg ggagtgaagg
     5701 cgagcgatac cctgacccgg ctcaagctgg ccaactcgct gatgtacctg acccggggcc
     5761 agccggtggt ctactacggc gacgagcagg gcttcgccgg ctccggcggc gacaaggcgt
     5821 cccggcagga catgttcgcc tcgaaggtga agtcgtacaa cgacgaagaa gtgatcggcg
     5881 gcgcgtccgg ctcgaaggat cggtacaaca ccaacgccac gctgtaccgc cagatcgcgc
     5941 aactggccaa gctgcgggcc gcgcatccgg cgctcgccga cggtgcccag atcgcgcggt
     6001 actcgagcaa cgaggcaggc atcttcgcgg tcagccgcat cgacaaggcg gccaaacgcg
     6061 aatatctggt ggtcaccaac aacgccgcga cctcgaagac ggccacgttc gccacctatt
     6121 cgcccagcat ccggttcgac tcgctgctgg gcggcggctc aggcgtgcgg gcggaccggt
     6181 tcggacgggt caccgtgacg gtgccggcat acggcaccac ggtcttgcgg gccaccggac
     6241 gcatgccggc tgcgtccgcg gcaccggtga ttctgccgct cgctcccaag agcggcggca
     6301 cggtcggcgg tcgcgccgaa atctctgccg cgctggccga caccagcgcg ttcgccgagg
     6361 tgagcttcta ctaccgtccg gcgggcacca gcgactggaa gctgatcggt accgacgaca
     6421 acccgcgcta ccgcgtctac caagacgtca gcggcatgcc caaggggtcg ctgctggagt
     6481 accgggtcgt ggccaaagac ctgcaggggc gttactcggc cacctcgacg tacggcttcg
     6541 tcggcgacgc cggcggcacc accgggccca gcactatcag ctacgacccg cccgagaagc
     6601 agcccgactc ggtgtcggtg cccggttcgc acaacgcgtc gatgggctgc accggcggcc
     6661 ccaacggcga cggcaactgg ttcccggtct gcccgcaggc ccagctgacc ctcgacgccg
//
LOCUS       DAOSUG010000109         6696 bp    DNA     linear   ENV 10-JUN-2024
DEFINITION  MAG TPA_asm: Micropruina sp. isolate AMR_MDS_2195
            NODE_5500_length_6696_cov_4.834362, whole genome shotgun sequence.
ACCESSION   DAOSUG010000109 DAOSUG010000000
VERSION     DAOSUG010000109.1
DBLINK      BioProject: PRJNA925477
            BioSample: SAMN32864262
            Sequence Read Archive: SRR4236673
KEYWORDS    WGS; ENV; Metagenome Assembled Genome; MAG; Third Party Data; TPA;
            TPA:assembly.
SOURCE      Micropruina sp. (wastewater metagenome)
  ORGANISM  Micropruina sp.
            Bacteria; Bacillati; Actinomycetota; Actinomycetes;
            Propionibacteriales; Nocardioidaceae; Micropruina.
REFERENCE   1  (bases 1 to 6696)
  AUTHORS   Abdulkadir,N., Saraiva,J.P., Zhang,J., Stolte,S., Gillor,O.,
            Harms,H. and Rocha,U.
  TITLE     Genome-centric analyses of 165 metagenomes show that mobile genetic
            elements are crucial for the transmission of antimicrobial
            resistance genes to pathogens in activated sludge and wastewater
  JOURNAL   Microbiol Spectr 12 (3), e0291823 (2024)
   PUBMED   38289113
REFERENCE   2  (bases 1 to 6696)
  AUTHORS   Abdulkadir,N.
  TITLE     Direct Submission
  JOURNAL   Submitted (24-MAR-2023) Environmental Microbiology, Helmholtz
            Center for Environmental Research -UFZ, Permoserstr 15, Leipzig
            04318, Germany
COMMENT     The annotation was added by the NCBI Prokaryotic Genome Annotation
            Pipeline (PGAP). Information about PGAP can be found here:
            https://www.ncbi.nlm.nih.gov/genome/annotation_prok/
            
            ##Genome-Assembly-Data-START##
            Assembly Method        :: SPAdes v. 3.11.1
            Genome Representation  :: Full
            Expected Final Version :: Yes
            Genome Coverage        :: Not Applicable
            Sequencing Technology  :: Illumina MiSeq
            ##Genome-Assembly-Data-END##
            
            ##Genome-Annotation-Data-START##
            Annotation Provider               :: NCBI
            Annotation Date                   :: 05/04/2023 04:57:10
            Annotation Pipeline               :: NCBI Prokaryotic Genome
                                                 Annotation Pipeline (PGAP)
            Annotation Method                 :: Best-placed reference protein
                                                 set; GeneMarkS-2+
            Annotation Software revision      :: 6.5
            Features Annotated                :: Gene; CDS; rRNA; tRNA; ncRNA
            Genes (total)                     :: 2,776
            CDSs (total)                      :: 2,750
            Genes (coding)                    :: 2,714
            CDSs (with protein)               :: 2,714
            Genes (RNA)                       :: 26
            tRNAs                             :: 24
            ncRNAs                            :: 2
            Pseudo Genes (total)              :: 36
            CDSs (without protein)            :: 36
            Pseudo Genes (ambiguous residues) :: 0 of 36
            Pseudo Genes (frameshifted)       :: 2 of 36
            Pseudo Genes (incomplete)         :: 32 of 36
            Pseudo Genes (internal stop)      :: 2 of 36
            ##Genome-Annotation-Data-END##
FEATURES             Location/Qualifiers
     source          1..6696
                     /organism="Micropruina sp."
                     /mol_type="genomic DNA"
                     /submitter_seqid="NODE_5500_length_6696_cov_4.834362"
                     /isolate="AMR_MDS_2195"
                     /isolation_source="Activated sludge of municipal
                     wastewater treatment plants"
                     /db_xref="taxon:2737536"
                     /environmental_sample
                     /geo_loc_name="Canada"
                     /lat_lon="49.25 N 123.1 W"
                     /collection_date="2015-06-16"
                     /metagenome_source="wastewater metagenome"
                     /note="metagenomic"
     gene            complement(<1..874)
                     /locus_tag="PLF56_05035"
     CDS             complement(<1..874)
                     /locus_tag="PLF56_05035"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_002549061.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="serine--tRNA ligase"
                     /protein_id="HOQ52959.1"
                     /translation="MIDPKLLRTQPDALRAAQEARGESVQLVDELIAADEARRSSIAA
                     FEALRAEQKTIGREVARAKGDEKVALITRTKTLSAQVKAAETAVGQAAGRYDELMLAL
                     PNPVFADVPRGGEDDFVIVETHGTPRDFAAEGFEPRDHLELGELLGAIDMPRGTKVSG
                     SRFYFLTGQGALLELALVNLAMAKAVEWGFTPILPPALVKPSAMEGTGFLGQAAQDVY
                     HLPADDLYLVGTSEVALAAFHSDEILDPGTLPMKYAGFSPCYRREAGSYGKDTRGIFR
                     VHWFDKVEMFVYCHP"
     gene            955..2508
                     /locus_tag="PLF56_05040"
     CDS             955..2508
                     /locus_tag="PLF56_05040"
                     /inference="COORDINATES: protein motif:HMM:NF039905.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_function: GO:0016301 - kinase activity [Evidence IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="diacylglycerol kinase family protein"
                     /protein_id="HOQ52960.1"
                     /translation="MRTRRLGLAPIATIGFGVGFALWTWLVFAGALTWLDALAAPPAQ
                     WQSPAVQIAAAVAVVCHPFVVYSALAGIAIWAFRRRLRNLTLAVLGSVALGWGGQEVL
                     RRVFQRERPPGSLPELITNQGYAYPSGHLVAVTVAALLVIATTTTTRQPRRVIELWRW
                     LGIGLILLVSADRWLLHAHYVSDLVGGLLWGGFAASLCLSIARVHMQPVRPVRAASKN
                     AETKRCAIVVNPTKIPDWAAFRRHVEFDCKQNGWQPMFLETSVDDPGRGMTRAAIEAN
                     VDLVLVAGGDGTVRVVCAELSGTDIPLGVLPSGTGNLLARNLSVPLDESAALEVVFHG
                     ERKRIDLVEVVIDDAEPPEHIAVMAGLGLDARIMSGTDDGLKKVIGPAAYVVAAGDAA
                     SLPSFDVTVTLDEAEAFERHAGLVMVGNVGSLQGNVQLLPDAQFDDGLLDLFVASPAN
                     VADWLRITTSVILPVPEASEIDRGQGRTVLIECTEPVEYQLDGDAAGECRTLRATVVP
                     QTLTVMVPD"
     gene            complement(2536..4821)
                     /locus_tag="PLF56_05045"
     CDS             complement(2536..4821)
                     /locus_tag="PLF56_05045"
                     /inference="COORDINATES: protein motif:HMM:NF012998.2"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_function: GO:0004664 - prephenate dehydratase activity
                     [Evidence IEA];
                     GO_process: GO:0009094 - L-phenylalanine biosynthetic
                     process [Evidence IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="prephenate dehydratase domain-containing
                     protein"
                     /protein_id="HOQ52961.1"
                     /translation="MTQTLAAGDPTRLVVVGAAAGMGRWLSDHLFAELPWQHVVLVDT
                     GQTEALLDGAAALYRDTPVRTGTLPEVADELARPGFVLCVAVPDSVAPGVLADLDALV
                     PSDAPIILVSVGFTWAMDVLASVPGRTAVAVHPLMDTGARSLDGQTVCATEVRGAATG
                     WLARAITSRGGIYTVLSPQWHDRIMTDVLAMTQQALLGFVTAVTDSELGLTDVWATRT
                     PLFEALLGLAVSVLEENQELTLAHVQASLHGDRAAERLARAAASVHGALGSREQFQHR
                     IAATRDRFSGALFDTVRTTAAATLSAGQSKRALLARVRRLGGLVGLHPSGRPDTLRVG
                     RLVELTPVHLVLEELLIGPKGSAALLSGPGVRNAKRLGRRGNPVRTRFGIGHVEVLAE
                     AELDVALDEWLAHVRRDVRFLVPESVAGEGIASVVREQRGVGEARLVSEAVRTGQRAV
                     VIRTGIRADLDLDETIERLRRAVEVAYAWPHGVARPVRASAPALRYLGPPGSFSESAA
                     RQFAVGLTGAGEHSVQIEPTGSFDDVLECTRTGGLGVLPVTSSASGLVSRSTRALLGC
                     DADLIAGGVVDVAVRFDAYAAAPVVLAELKGAPVFSHPQALAQCANFITRWGLVAQPC
                     ASTTDALYRLREVRGPAIAIASSGAEGDHGFVHVVEREIDDLSGSITRFLIVGQPDAF
                     AEHRDGSDPTLRRIVLAPSVASIGHLIGRGAGFDELLTDAEGQCLWVSSQVSVELPHG
                     VRDLGVVPWSPRTPVVRPTPG"
     gene            complement(4844..5581)
                     /locus_tag="PLF56_05050"
     CDS             complement(4844..5581)
                     /locus_tag="PLF56_05050"
                     /inference="COORDINATES: ab initio
                     prediction:GeneMarkS-2+"
                     /note="Derived by automated computational analysis using
                     gene prediction method: GeneMarkS-2+."
                     /codon_start=1
                     /transl_table=11
                     /product="hypothetical protein"
                     /protein_id="HOQ52962.1"
                     /translation="MDDPRRRPLPAHLGRWALGVVAVLLVAIVALGVIGGRLGPPLPA
                     ATVPAPTPGPASMLPSPPTNAVVVDEATRELTVAPYLTGRMPGRPFAFDAPTTVKGMF
                     GEVVFGGVTSDPDWNQNEDLPATIVLGQLEPAAVIEGDLPGTARGVVAEFSRRSYHTL
                     EDLSVHDVRSDDPSTIGAYPAQYAHAEVTGKLSSGVTERVSVSLLLVSLPNARTFAFV
                     GVKPDRPTATPHFAAIDAAAASIRPAA"
     gene            complement(5603..>6696)
                     /locus_tag="PLF56_05055"
     CDS             complement(5603..>6696)
                     /locus_tag="PLF56_05055"
                     /inference="COORDINATES: ab initio
                     prediction:GeneMarkS-2+"
                     /note="Derived by automated computational analysis using
                     gene prediction method: GeneMarkS-2+."
                     /codon_start=3
                     /transl_table=11
                     /product="hypothetical protein"
                     /protein_id="HOQ52963.1"
                     /translation="PVMRWVEAPWQDTPSVYGPIASFSQWLAATLGGESMHQVVFWLQ
                     MFALIPFLIVGLIVVKMAHGDPAFQTRAVLFTVLNPIMIWSICAQAHNEPLTLVFAVA
                     GFWFLRRNAWLAGLGIGLAGCVKVSLVYYGLAMLWGYRHQPRKLLQLCVAAAGTIGLG
                     YGLFAPQALFAAARNTSYVSAGSWTEWLHALGTVTLGDATSRRIMSALGLIGLFVIGW
                     MLSRVLPWVTAPGAAPGTDPRRDPLTITVRTAALLTAAWLITSPYTLSWYDLITWVPL
                     ALMTANRLDAITMWRGAWLSLAYVTGRVIEFGPGVVVAGFIMRDVLCTAAQVTAIVWV
                     IWWWRQEGGELPDVRIVRDGWRRLRGRPA"
BASE COUNT         1025 a         2394 c         2371 g          906 t
ORIGIN      
        1 cggggtggca gtagacgaac atctcgacct tgtcgaacca gtgcacccgg aagattcccc
       61 tggtgtcctt gccgtaactg ccggcctcgc ggcggtagca cggcgagaag ccggcgtact
      121 tcatcggaag cgtgccgggg tcgagaatct cgtcgctgtg aaaggcggcc agcgccacct
      181 cggaggtgcc gaccaggtac aggtcgtcgg cgggcaggtg gtagacgtcc tgggcggcct
      241 ggccgaggaa gccggtgccc tccatcgccg agggcttgac cagcgccggc ggcagaatcg
      301 gcgtgaaacc ccactcgacc gccttggcca tggccaggtt gaccagggcg agttcgagca
      361 acgccccttg tccggtgaga aagtagaagc gcgagcccga caccttggtg ccgcggggca
      421 tgtcgatcgc gccgagcagt tcgccgagtt cgaggtggtc gcgcggttcg aagccctcgg
      481 ccgcgaagtc gcgcggggtg ccgtgggtct cgacgatcac gaagtcgtcc tcgccgccgc
      541 ggggcacgtc ggcgaacacc gggttgggca gggcgagcat cagttcgtcg tagcgcccgg
      601 ccgcctgacc gaccgcggtc tcggcggcct tgacctgggc cgacagcgtc ttggtgcggg
      661 tgatcagcgc caccttctca tcgcccttgg cccgcgccac ctcgcgtccg atcgtcttct
      721 gctcggcccg cagggcctcg aacgccgcga tggacgagcg gcgcgcctcg tccgcggcga
      781 tcagttcatc caccagttgg acggactcgc cgcgcgcctc ctgcgcggct cgcagggcat
      841 cgggttgggt gcgcagcaac ttcgggtcga tcacccggtc agcctatcgg caccggcccg
      901 cgccacaccg ttccgggcga ccgggcgggc cgatcgcggg tgccacaatg gcgcatgcgc
      961 acacgccggc tgggcctcgc accgatcgcc accataggtt tcggcgtcgg gttcgccctg
     1021 tggacctggc tggtcttcgc cggcgcactg acgtggctgg acgcactcgc ggccccaccc
     1081 gctcagtggc agagcccggc ggtgcagatc gccgcagccg ttgcggtggt gtgccatccg
     1141 ttcgtggtct acagcgcgct ggccggcatc gcgatctggg cgttccggcg ccggctgcgc
     1201 aacctgaccc tggcggtgct cggttcggtc gccctgggct ggggcggcca ggaggtgctg
     1261 cgccgggtgt tccagcgcga gcgtcccccg ggttcgctgc ccgagctgat caccaatcag
     1321 ggctacgcct acccgtccgg gcatctggtg gcggtgaccg tcgccgccct gctggtgatc
     1381 gccaccacca ccaccacccg gcagccgcgc cgggtgatcg aactgtggcg ctggctcggc
     1441 atcggcctga tcctgctggt gtccgctgat cgctggctgt tgcatgccca ctacgtcagc
     1501 gatctggtcg gcgggctgct gtggggcggg ttcgcggcca gcctgtgcct cagcatcgcg
     1561 cgggtgcaca tgcagcccgt ccgtccggtt cgggccgcga gcaagaatgc cgagaccaag
     1621 cggtgcgcga tcgtggtgaa cccgaccaag attcccgatt gggccgcctt ccgccggcat
     1681 gtcgagttcg actgcaagca gaacggctgg cagccgatgt ttctcgaaac ctcggtcgat
     1741 gaccccggac gtggcatgac gcgggccgcg atcgaggcga acgtcgacct ggtgctggtg
     1801 gccggcggcg acggaacggt gcgggtggtg tgcgcggaac tgtcgggtac cgacattccg
     1861 ctcggtgtgc tgccgtcggg caccggcaac ctgctggcgc gcaacctgag cgtcccgctg
     1921 gacgagtccg ccgcgctcga ggtggtcttt cacggcgaac gcaagcgcat cgacctggtc
     1981 gaggtggtca tcgatgacgc ggagcccccc gaacacatcg cggtgatggc cggcctgggc
     2041 ctggacgccc gcatcatgag cggcaccgac gacgggctga agaaggtgat cggcccggcc
     2101 gcctacgtgg tggcggccgg cgatgccgcc agcctgccgt cgttcgacgt caccgtgacc
     2161 ctcgacgagg ccgaagcgtt cgagcggcat gccggcctgg tcatggtggg caatgtcggc
     2221 agcctgcagg gcaacgtgca actgctgccc gatgcgcagt tcgacgacgg cctgctcgac
     2281 ctgttcgtcg cctcgcccgc gaacgtggcc gactggctgc ggatcaccac cagcgtgatt
     2341 ctgccggtgc cggaggcgtc cgagatcgac cgcgggcagg gccgaacggt gctgatcgag
     2401 tgcacggaac ccgtcgagta ccagctggac ggcgacgcgg ccggcgaatg tcgaacgctg
     2461 cgggcgacgg tggtgccgca gacgctcacc gtgatggtgc ccgactgatc ggctgaccgg
     2521 cgctctccag ggcggtcagc cgggcgtcgg acgcaccacc ggcgtccgcg gcgaccaggg
     2581 caccacgccc aggtcgcgca ccccgtgcgg aagctcgacg ctgacctggc tgctcaccca
     2641 caggcactgc ccctcggcgt cggtgagcag ctcgtcgaag ccggcacccc gtccgatcag
     2701 gtgaccgatg gaggccaccg acggcgccag cacgatccgt cgcagcgtgg ggtcggagcc
     2761 gtcccgatgc tccgcgaagg cgtccggctg cccgacgatc agaaacctgg tgatcgagcc
     2821 cgacaggtcg tcgatctcgc gttccaccac atggacgaac ccgtggtcgc cctcggcccc
     2881 cgacgacgcg atcgcgatcg ccgggccgcg cacctcgcgc agccggtaca gcgcgtcggt
     2941 ggtcgaggcg cagggctgcg caaccagccc ccagcgggtg atgaagttgg cgcactgggc
     3001 cagggcctgc gggtgactga acaccggcgc gcccttcagc tcggccagca ccaccggagc
     3061 ggccgcatac gcgtcgaagc gcaccgccac gtcgaccacg ccaccggcga tcaggtcggc
     3121 gtcgcagccc aacaatgccc gggttgagcg gctgaccaga cccgatgcag aactggtgac
     3181 cggcagcacc cccaagccgc cggtgcgggt gcactcgagc acgtcgtcga acgaaccggt
     3241 cggctcgatc tgcaccgaat gctcccccgc gccggtcaac ccgaccgcga actggcgggc
     3301 ggcgctttcg gaaaagctgc cgggcggccc caggtagcgc agcgccggcg ctgaggcgcg
     3361 caccggtcgg gccactccgt gcggccaggc gtaggccacc tcgacggcgc ggcgcagtcg
     3421 ttcgatggtt tcgtccaggt cgaggtcggc ccggatgccg gtgcggatca ccaccgcccg
     3481 ctgccccgtg cgcacggcct cggagaccag ccgggcctca ccgacgccgc gctgctcgcg
     3541 caccaccgac gcgatgccct cgccggccac ggattcgggc accagaaacc ggacgtcgcg
     3601 gcgcacgtgc gccagccact cgtccagggc gacgtcgagt tcggcctcgg ccagcacctc
     3661 gacgtgcccg atgccgaacc gggtgcggac cgggttgccg cgccggccca gccgcttcgc
     3721 gttgcggacg ccggggcccg acagcagcgc cgccgaaccc ttcggcccga tcagcagctc
     3781 ttcgagcacc aggtgcacgg gagtcagctc gaccagccgg cccacccgca gcgtgtcggg
     3841 gcgtccgctt gggtgcagcc cgaccagccc gcccagccgg cggacccgag ccagcagcgc
     3901 ccgcttcgac tggccggcac tcagggtggc ggcggcggtg gtccgcacgg tgtcgaacag
     3961 cgcgccgctg aaccggtccc gggtagcggc gatccggtgt tggaactgct cacgcgaacc
     4021 gagcgcccca tgaaccgacg ccgccgcccg agccaggcgt tcggccgccc gatcgccgtg
     4081 caggctggcc tgcacgtgag ccagcgtcag ctcctggttc tcctccagca cgctgaccgc
     4141 caggccgagc agtgcctcga acagcggcgt acgggtggcc catacgtcgg tgaggcccag
     4201 ttcgctgtcg gtgacggcgg tgacgaagcc gagcagcgcc tgctgcgtca tcgcgagcac
     4261 gtcggtcatg atgcggtcgt gccactgcgg cgagagcacg gtgtagatcc cgccccgcga
     4321 ggtgatggcg cgggccagcc agccggtcgc cgctcctcga acctccgtgg cgcacacggt
     4381 ctggccgtcc agcgagcggg ctccggtgtc catcagcggg tgcaccgcga ccgcggtgcg
     4441 cccgggcacc gaggccagca cgtccatcgc ccaggtgaag ccgacgctga ccagaatgat
     4501 cggcgcatcg gacggcacca gtgcgtccag gtcggccagc acgccgggcg ccacgctgtc
     4561 gggcaccgcg acgcacagca cgaagccggg ccgggccaac tcgtctgcga cctcgggcaa
     4621 cgtgccggta cgcacggggg tgtcgcggta gagggccgcc gcgccgtcca gcagcgcctc
     4681 ggtctggccg gtgtcgacca gcaccacgtg ctgccagggc agctcggcga acagatgatc
     4741 cgacagccag cgacccatgc cggccgcggc ccccaccacc accagccggg tcgggtcgcc
     4801 cgcagccagg gtctgggtca ccgcagcagt ctagggcgtg cgttcatgcg gccggcctga
     4861 tggacgccgc cgccgcgtcg atggcggcga agtgcggcgt cgccgtcggc cggtccggct
     4921 tcaccccgac gaaggcgaag gtgcgcgcgt tgggcaggct gaccagcaac agcgaaaccg
     4981 agacgcgctc ggtcacgccg ctgctcagct tgccggtcac ctcggcgtgc gcgtactgcg
     5041 cgggatacgc gccgatcgtg gacggatcgt cggagcgcac gtcgtgcacg ctgagatcct
     5101 ccagcgtgtg gtagctgcgc cggctgaact cggccaccac cccgcgggcc gtgccgggca
     5161 ggtcgccctc gatcaccgcg gcgggttcca gttggccgag cacgatggtg gccggcaggt
     5221 cctcgttctg gttccagtcg ggatcggagg tgacgccgcc gaagaccacc tcgccgaaca
     5281 tgcccttgac cgtggtgggg gcgtcgaacg cgaacggacg gccgggcatc cgtccggtca
     5341 ggtacggcgc gaccgtgagc tcacgggtcg cctcgtcgac caccaccgca ttggtcggag
     5401 gcgagggcag catcgaggcc gggccgggcg tgggtgcggg aacggtggcg gccggcagcg
     5461 gaggaccgag gcgtccaccg atcacgccga gagcaacgat cgccacgagc agcacagcga
     5521 cgaccccaag cgcccagcga cccaggtggg ccggcagcgg tctgcgcctc ggatcatcca
     5581 cggccaggag tctaggccga ggctaggccg gacgtccgcg cagccgccgc cacccgtcgc
     5641 gcacgatccg gacgtcgggc agctcgccgc cctcttgacg ccaccaccag atcacccaca
     5701 cgatggcagt gacctgcgcg gcggtgcaca acacgtcacg catgatgaag cccgccacca
     5761 cgacgcccgg gccgaactcg atcacccgtc cggtcacgta ggccagcgac agccaggcgc
     5821 cgcgccacat ggtgatcgcg tcgagccggt tggccgtcat cagcgccagc ggcacccagg
     5881 tgatcaggtc gtaccagctc agcgtgtagg gcgaggtgat cagccacgcg gcggtcaaca
     5941 gagcggcggt gcgcaccgtg atggtcagcg ggtcacggcg cggatcggtg cccggcgccg
     6001 cgcccggagc ggtcacccag ggcagcaccc gggagagcat ccagccgatc acgaacagcc
     6061 cgatcaggcc cagcgcgctc atgatgcgcc gcgacgtcgc gtcgccaagg gtcaccgtgc
     6121 cgagggcgtg cagccattcg gtccacgagc cggccgagac gtagctggtg ttgcgcgcgg
     6181 cggcgaagag cgcctggggt gcgaacaggc catacccgag cccgatggtg cccgccgccg
     6241 ccacgcacaa ctgcagcagc ttgcgcggct ggtgccggta gccccagagc attgccagcc
     6301 cgtaatagac cagcgagacc ttgacgcagc cggccagccc gatgcccagg ccggccagcc
     6361 aggcgttgcg gcgcagaaac cagaagccgg ccacggcgaa caccagggtc agcggctcat
     6421 tgtgcgcctg ggcgcagatg gaccagatca tgatcgggtt cagcaccgtg aacagcacgg
     6481 cccgggtctg aaaggccggg tcgccgtggg ccatcttgac cacgatcagg ccgacgatca
     6541 agaacgggat cagcgcgaac atctgcagcc agaacaccac ctggtgcatg ctctcgccgc
     6601 ccagggtggc ggccagccac tgactgaacg acgcgatcgg gccgtacacc gagggggtgt
     6661 cctgccaggg cgcctcgacc cagcgcatca ccgggt
//
LOCUS       DAOSUG010000110         6634 bp    DNA     linear   ENV 10-JUN-2024
DEFINITION  MAG TPA_asm: Micropruina sp. isolate AMR_MDS_2195
            NODE_5552_length_6634_cov_4.009728, whole genome shotgun sequence.
ACCESSION   DAOSUG010000110 DAOSUG010000000
VERSION     DAOSUG010000110.1
DBLINK      BioProject: PRJNA925477
            BioSample: SAMN32864262
            Sequence Read Archive: SRR4236673
KEYWORDS    WGS; ENV; Metagenome Assembled Genome; MAG; Third Party Data; TPA;
            TPA:assembly.
SOURCE      Micropruina sp. (wastewater metagenome)
  ORGANISM  Micropruina sp.
            Bacteria; Bacillati; Actinomycetota; Actinomycetes;
            Propionibacteriales; Nocardioidaceae; Micropruina.
REFERENCE   1  (bases 1 to 6634)
  AUTHORS   Abdulkadir,N., Saraiva,J.P., Zhang,J., Stolte,S., Gillor,O.,
            Harms,H. and Rocha,U.
  TITLE     Genome-centric analyses of 165 metagenomes show that mobile genetic
            elements are crucial for the transmission of antimicrobial
            resistance genes to pathogens in activated sludge and wastewater
  JOURNAL   Microbiol Spectr 12 (3), e0291823 (2024)
   PUBMED   38289113
REFERENCE   2  (bases 1 to 6634)
  AUTHORS   Abdulkadir,N.
  TITLE     Direct Submission
  JOURNAL   Submitted (24-MAR-2023) Environmental Microbiology, Helmholtz
            Center for Environmental Research -UFZ, Permoserstr 15, Leipzig
            04318, Germany
COMMENT     The annotation was added by the NCBI Prokaryotic Genome Annotation
            Pipeline (PGAP). Information about PGAP can be found here:
            https://www.ncbi.nlm.nih.gov/genome/annotation_prok/
            
            ##Genome-Assembly-Data-START##
            Assembly Method        :: SPAdes v. 3.11.1
            Genome Representation  :: Full
            Expected Final Version :: Yes
            Genome Coverage        :: Not Applicable
            Sequencing Technology  :: Illumina MiSeq
            ##Genome-Assembly-Data-END##
            
            ##Genome-Annotation-Data-START##
            Annotation Provider               :: NCBI
            Annotation Date                   :: 05/04/2023 04:57:10
            Annotation Pipeline               :: NCBI Prokaryotic Genome
                                                 Annotation Pipeline (PGAP)
            Annotation Method                 :: Best-placed reference protein
                                                 set; GeneMarkS-2+
            Annotation Software revision      :: 6.5
            Features Annotated                :: Gene; CDS; rRNA; tRNA; ncRNA
            Genes (total)                     :: 2,776
            CDSs (total)                      :: 2,750
            Genes (coding)                    :: 2,714
            CDSs (with protein)               :: 2,714
            Genes (RNA)                       :: 26
            tRNAs                             :: 24
            ncRNAs                            :: 2
            Pseudo Genes (total)              :: 36
            CDSs (without protein)            :: 36
            Pseudo Genes (ambiguous residues) :: 0 of 36
            Pseudo Genes (frameshifted)       :: 2 of 36
            Pseudo Genes (incomplete)         :: 32 of 36
            Pseudo Genes (internal stop)      :: 2 of 36
            ##Genome-Annotation-Data-END##
FEATURES             Location/Qualifiers
     source          1..6634
                     /organism="Micropruina sp."
                     /mol_type="genomic DNA"
                     /submitter_seqid="NODE_5552_length_6634_cov_4.009728"
                     /isolate="AMR_MDS_2195"
                     /isolation_source="Activated sludge of municipal
                     wastewater treatment plants"
                     /db_xref="taxon:2737536"
                     /environmental_sample
                     /geo_loc_name="Canada"
                     /lat_lon="49.25 N 123.1 W"
                     /collection_date="2015-06-16"
                     /metagenome_source="wastewater metagenome"
                     /note="metagenomic"
     gene            complement(<1..772)
                     /locus_tag="PLF56_05060"
     CDS             complement(<1..772)
                     /locus_tag="PLF56_05060"
                     /inference="COORDINATES: protein motif:HMM:NF012335.2"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="zinc-binding dehydrogenase"
                     /protein_id="HOQ52964.1"
                     /translation="MSPSMMAVVAHAGRSPEDTFTVVEVTVPELRPHDLLVRVRAVSV
                     NPVDTKVLKRLPPDGSVILGYDAAGVVEAVGSDVTGFSVGDEVYYAGDNTRPGSNAEL
                     HAVDERIVARKPTGLDWAEAAALPLTTITAWESLFDHLSLRSDSRGSLLIVGGAGGVG
                     SVMIQLAKALTGVTVIATAGREESCAWVHELGADHCVGHRDLVAEVAPLAPEGLDWVF
                     SSHTPGNIDAYATLLKPFGAIVAVDDDPGASRAIARDLT"
     gene            complement(966..3797)
                     /locus_tag="PLF56_05065"
     CDS             complement(966..3797)
                     /locus_tag="PLF56_05065"
                     /EC_number="1.17.4.1"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_013863026.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_function: GO:0004748 - ribonucleoside-diphosphate
                     reductase activity, thioredoxin disulfide as acceptor
                     [Evidence IEA];
                     GO_process: GO:0006260 - DNA replication [Evidence IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="vitamin B12-dependent ribonucleotide reductase"
                     /protein_id="HOQ52965.1"
                     /translation="MTETARASSRRSSKPSGLTIARVFTTDGVHPYDQVTWEHRDVVQ
                     TNWKTGETVFEQHGAEFPDFWSVNASTIVTTKYFRGAVNTAARETSLRQLIDRVVHSY
                     RRAGESNGYFATENDAEIFEHELTWMLLHQYFSFNSPVWFNVGTKSPQQVSACFILSV
                     DDSMDSILNWYKEEGFIFKGGSGAGLNLSRIRSSKELLSSGGTASGPVSFMRGADASA
                     GTIKSGGATRRAAKMVVLDVDHPDIEEFIDTKAREEDKIRALRDAGFDMDLGGKDIVS
                     VQYQNANNSVRVSDEFMQAVVEGTEFGLRARMTGEVIEKVDARELFGKMAKAAWECAD
                     PGIQYDDTINAWHTNPETGRITASNPCSEYMSLDNSSCNLASLNLLKFLRDDNTFDVA
                     NFVKAVELVITAMDISICFADFPTESIGQTTRDYRQLGIGYANLGALLMAIGRGYDSA
                     GGRALAASITSLMTATSYRRSAELAAIVGPYTGYARNATAHQKVMRKHQAANDEVRTF
                     NEVDGSLHEAATKEWASVVALGAGNGFRNAQASVLAPTGTIGFMMDCDTTGIEPDFSL
                     VKFKKLVGGGSMQIVNQTIPRALHQLGYTEETIEAIIEYIAQHGHVVNAPGLKGEHYE
                     IFDTAMGERAIKPMGHVQMMAAVQPFLSGAISKTVNLPETATIDEIADVYLQGWKLGL
                     KALAVYRDNCKVGQPLSDGKKQPEKVADEVPVRVEYRPRRKRLPKSRMSRTTSFSVAG
                     AEGYLTAGAYDDGRLGEVFLKLGKQGSTLAGVMDAFSIAISIALQYGVPLETYVQKFT
                     NMKFEPAGMTDDADVRMAQSIIDYIFRRLALDYLTFDERAELGIHTSAERARYVETGS
                     YLSEEDEANLIDSEALKNDAGDEVTIHEDGPNQPALLEARPHSTAEVLEKISGLAIDA
                     PLCMTCGTKMRRSGSCYICEGCGSTSGCS"
     gene            complement(3834..4292)
                     /gene="nrdR"
                     /locus_tag="PLF56_05070"
     CDS             complement(3834..4292)
                     /gene="nrdR"
                     /locus_tag="PLF56_05070"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_015070319.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_function: GO:0140110 - transcription regulator activity
                     [Evidence IEA];
                     GO_process: GO:0006355 - regulation of DNA-templated
                     transcription [Evidence IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="transcriptional regulator NrdR"
                     /protein_id="HOQ52966.1"
                     /translation="MYCPYCRHTDSRVLDSRVAEDGAAIRRRRQCPACERRFTTLEQM
                     QLVVVKRSGVVEPFSRDKVVSGVRKACKGRPVSEADLARLGQLVEETLRASGQAEIPA
                     HEVGLAILGPLSTLDAVAYLRFASVYKQYSSVEDFEAEIDNLRSQLLSAR"
     gene            complement(4920..5120)
                     /locus_tag="PLF56_05075"
     CDS             complement(4920..5120)
                     /locus_tag="PLF56_05075"
                     /inference="COORDINATES: ab initio
                     prediction:GeneMarkS-2+"
                     /note="Derived by automated computational analysis using
                     gene prediction method: GeneMarkS-2+."
                     /codon_start=1
                     /transl_table=11
                     /product="hypothetical protein"
                     /protein_id="HOQ52967.1"
                     /translation="MRTRPVVWQPAVQHGLQLTRRGLALAVGLFLGMVAAATITLVVG
                     FFSVSNAPVTTPSAPGSSVVGG"
     gene            5760..6533
                     /gene="lexA"
                     /locus_tag="PLF56_05080"
     CDS             5760..6533
                     /gene="lexA"
                     /locus_tag="PLF56_05080"
                     /EC_number="3.4.21.88"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_013863021.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_function: GO:0004252 - serine-type endopeptidase
                     activity [Evidence IEA];
                     GO_process: GO:0006282 - regulation of DNA repair
                     [Evidence IEA];
                     GO_process: GO:0006355 - regulation of DNA-templated
                     transcription [Evidence IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="transcriptional repressor LexA"
                     /protein_id="HOQ52968.1"
                     /translation="MTVGRPSNAEVAQQLEMAKITQLPDGPADTEGLTPRQRRILELI
                     HLAVSTRGYPPTIREIGEAAGLASPSSVSHQLRALEAKGYLRRDPHRPRAIEVRFPES
                     LSAMEPDSSELVADGVVEHEPDVTGIGDAMPEAVNVPVVGRIAAGGPILAEERVEDVF
                     PLPKQLVGDGTLFLLEVRGDSMIEAAICDGDFVVVRQQPTANNGDIVAALLEDEATVK
                     TFKRTDGQVWLMPHNPAYSPIDGNHASILGKVVAVLRRV"
BASE COUNT         1069 a         2238 c         2190 g         1137 t
ORIGIN      
        1 tggtcaggtc gcgggcgatc gcgcgcgacg cgccggggtc gtcgtcgacc gccacgatgg
       61 caccgaacgg cttgagcagc gtcgcgtacg catcgatgtt gcccggcgtg tgcgacgaga
      121 acacccaatc gagaccctcc ggtgcgagcg gagcgacctc ggctaccagg tcgcggtgcc
      181 cgacgcagtg gtcggcaccg agctcatgca cccacgcgca ggactcctcg cgcccggccg
      241 tggcgatcac ggtcacgccg gtcaacgcct tcgccaactg gatcatcacg ctgccgaccc
      301 caccggcccc gcccacgatg agtagcgaac ccctactgtc gctacgcagg gacaggtggt
      361 cgaacagcga ctcccatgcg gtgatcgtgg tcaacggcag cgcagccgcc tcggcccagt
      421 ccagtccggt cggcttgcgg gccacgatgc gctcatcaac cgcgtgcagc tcggcgttgc
      481 tgccgggccg ggtgttgtcg cccgcgtagt acacctcgtc gccgacgctg aacccggtca
      541 cgtcggagcc cacggcctcg accacaccgg ccgcgtcgta acccagaatg acgctgccgt
      601 cgggtggcag tcgcttcagc accttggtgt cgacggggtt caccgacact gcccgcaccc
      661 gcaccagaag gtcgtgcgga cgcagttcgg gaaccgtcac ctcgacgaca gtgaaggtgt
      721 cttcggggga acgaccggcg tgggccacca ccgccatcat gctcgggctc atggacgtac
      781 tccttcggtg caggtcgagg cactgcgtgg tgcagggacg tctctcaccc tatgacctgt
      841 gcgtcgtggc gcgctgccgc gcgaacacgc tccggctgac gccggacgca cgaaggggcc
      901 cggctcacac cggacccctt cacgtctact ccccgcgccg gtaccccccg ttggcgctgg
      961 ggcgatcagc tgcagccgct ggtgctgccg catccctcgc agatgtagca gctaccgctg
     1021 cgccgcatct tggtgccgca cgtcatgcac agcggcgcat cgatggccag gcccgagatc
     1081 ttctcgagca cctcggccgt cgagtgggga cgggcctcga gcagagccgg ctggttggga
     1141 ccgtcctcat ggatcgtgac ctcgtcgccc gcgtcgttct tcaacgcctc ggagtcgatc
     1201 aggttggcct cgtcctcttc ggacaggtac gacccggtct cgacgtagcg ggcccgctcg
     1261 gccgaggtgt ggatgcccaa ctccgcacgc tcgtcgaagg tcaggtagtc gagtgccaga
     1321 cggcggaaga tgtagtcgat gatcgactgc gccatgcgga cgtcggcgtc gtcggtcatg
     1381 ccggccggct cgaacttcat gttggtgaac ttctgcacgt aggtctcgag gggcaccccg
     1441 tactgcaggg cgatcgagat cgcgatcgag aaggcgtcca tcacgccggc cagggtcgag
     1501 ccctgcttgc cgagcttgag gaacacctcg ccgaggcgtc cgtcgtcgta ggcgcccgcg
     1561 gtcaggtagc cttccgcgcc cgcgaccgag aacgaggtgg tgcggctcat ccgcgacttg
     1621 ggcagacgct tgcggcgcgg ccggtactcg acccgcaccg gcacctcgtc ggcgaccttc
     1681 tcgggttgct tcttgccgtc gctgagcggc tgacccacct tgcagttgtc gcggtagacc
     1741 gccagtgctt tcaggccgag cttccagccc tgcaggtaca cgtcggcgat ctcgtcaatc
     1801 gtggccgtct cgggcaggtt gacggtcttg ctgatcgcac cggacaggaa cggctgcacc
     1861 gctgccatca tctgcacgtg acccatcggc ttgatggccc gctcgcccat cgcggtgtcg
     1921 aagatctcgt agtgttcgcc cttgagcccg ggcgcgttca ccacatgccc gtgttgggcg
     1981 atgtactcga tgatcgcctc gatggtctct tcggtgtagc ccagttggtg cagcgcccgc
     2041 ggaatggtct ggttgacgat ctgcatcgaa ccgccgccga ccagcttctt gaacttgacc
     2101 agcgagaagt ccggctcgat gccggtggtg tcgcagtcca tcatgaagcc gatcgtgccg
     2161 gtcggtgcca gcaccgaggc ctgggcgttg cggaagccgt tgcccgcacc cagcgcgacc
     2221 acggaggccc actccttggt ggccgcttcg tgcagcgagc cgtccacctc gttgaatgtg
     2281 cgcacctcgt cgttggcggc ctggtgcttg cgcatcacct tctgatgagc ggtggcgttg
     2341 cgtgcatagc cggtgtacgg gcccacgatc gctgccagtt cggccgagcg ccggtagctg
     2401 gtggccgtca tcagcgaggt gatggacgcg gccagcgccc gtccgccggc ggagtcgtag
     2461 ccccgtccga tggccatcag cagggcgccg agattggcgt aaccgatgcc gagctgccgg
     2521 tagtcgcggg tggtctgccc gatcgattcg gtcggaaagt ccgcgaagca gatcgagatg
     2581 tccatggctg tgatgaccag ctcgacggcc ttgacgaagt tcgccacgtc gaaggtgttg
     2641 tcgtcgcgca ggaacttgag caggttgagg ctggccaggt tgcacgagct gttgtcgagg
     2701 ctcatgtact ccgagcaggg gttcgacgcg gtgatgcgcc cggtctcggg gttggtgtgc
     2761 caggcgttga tcgtgtcgtc gtactggatg cccgggtcgg cgcactccca ggccgccttc
     2821 gccatcttgc cgaacagctc gcgggcgtcg accttctcga tcacctcgcc ggtcatccgg
     2881 gcgcgcagcc cgaactcggt gccctcgacc acggcctgca tgaactcgtc actgacccgc
     2941 acggagttgt tggcgttctg gtactgcacg ctgacgatgt ctttgccgcc caggtccatg
     3001 tcgaagccgg cgtcgcgcag ggcacggatc ttgtcttctt cgcgcgcctt ggtgtcgatg
     3061 aactcttcga tgtcgggatg atcgacgtcg agcaccacca ttttggccgc gcgccgggtg
     3121 gcgccgccgg acttgatggt gccggcggac gcgtcggcgc cgcgcatgaa gctgaccgga
     3181 ccggatgcgg tgccgcccga ggagagcagc tccttggacg agcggatgcg cgacaggttc
     3241 agcccggcac ccgaaccacc cttgaagatg aagccctcct ccttgtacca gttgaggatc
     3301 gaatccatcg agtcgtcgac gctgaggatg aagcaggcgc tgacctgctg cggcgacttg
     3361 gtgccgacgt tgaaccacac cggcgagttg aagctgaagt actggtgcag cagcatccag
     3421 gtgagttcgt gctcgaagat ctcggcgtcg ttctcggttg cgaagtagcc gttgctctcg
     3481 ccggcccgtc ggtagctgtg caccacccga tcgatcagct ggcgcaggct ggtctcgcgc
     3541 gccgcggtgt tgaccgcgcc ccggaagtat ttggtggtca cgatcgtgga ggcgttcacc
     3601 gaccagaagt cgggaaactc ggcaccgtgc tgctcgaaga cggtctcacc ggtcttccaa
     3661 ttcgtctgca cgacgtcacg gtgctcccag gtgacctggt cgtaggggtg caccccgtcg
     3721 gtggtgaaga cgcgcgcgat ggtcaatccc gagggcttgc tggagcgccg ggacgaggca
     3781 cgtgcggttt cggtcattgt gatgccttcc ttcgaaatgg gtgcggctgg cgctcaacgc
     3841 gcggagagca actgactacg gaggttgtcg atctcggcct cgaaatcttc aaccgatgag
     3901 tactgcttgt agacggacgc gaagcgcagg taggcgacgg cgtccagcgt gctgagcggg
     3961 cccaggatgg cgagcccgac ttcatgcgca ggaatctctg cctgaccgct ggcccggagc
     4021 gtctcttcga ccaactggcc gaggcgggcc aggtcggcct cactgaccgg ccggcccttg
     4081 caggccttgc ggacgccgct cacgaccttg tcgcgcgaga acggctcgac cacgccggag
     4141 cgcttcacca cgacgagctg catctgctcc aacgtggtga accggcgttc acacgccggg
     4201 cactggcggc gacgacggat ggctgcaccg tcttcggcga cccgcgaatc gagcacccga
     4261 gagtcggtgt gacggcagta cggacagtac atttcgcctt ggcctccttc gcttcgatcc
     4321 gacgctcatt aacgagtcgc cctctgtgcg cacccaaccc ctgtggagtg gggtgtgcac
     4381 tggctgtgga tatcgctgtg gacggaacca caatctgtgg atgaaccaca cgcctgtaac
     4441 tactacatct agagcttatg ccggatggag gcatgacgca acagacgacg gggatcagtc
     4501 gtcgagcctt cttgcgggcc cgtccaggcc gaggcccgga cccgaacagc ccacccgatc
     4561 ggccacgcgg aagtctcaca gcgctcgccc ggacgctgcc ggacccgtcg gcttcacggc
     4621 tgctcaagcc atgcgccacc gaggtcgtga cgggatgcgg caagcaccgg tatggagggc
     4681 accgagagca tgcgccgcgg ctgagccagc cgatgttcgg cccacgccga ccttgcctgc
     4741 ccttgactgt gggtgcgcgc ctgcgggaac acacccatgg ctcacaccgg ccggtcgatg
     4801 tcggccacga ccgaggtggc accccgatac cgcgacggcc cacgtaggtc tcgccaggcc
     4861 gagccaggct cgaccggtac cgacacccgg gcttcaggga tctgcggctc ggctcagcgt
     4921 cagccgccga ccaccgacga gcccggggca gacggggttg tgacgggagc gttggagacc
     4981 gaaaagaagc cgaccaccag cgtgattgtc gccgcggcca ccatgcccag gaacaggccc
     5041 actgccaacg ccagcccacg ccgggtcagc tggagaccat gctgaaccgc gggctgccag
     5101 accacgggcc tggtgcgcac ccacggcccc gaagaggtga cgggccgacc acccggagcc
     5161 ccaggatgag tgggccgggc cagcggcccc cgcgtctgcg gcacgtggtg ccacttcggc
     5221 cgagtcgggg tcagccggct gcccactgtc tcgtcaattg tcagtgcact catctcgcac
     5281 gtcctctctt cgaacatatg ttcgatgcca ttgttactgt tgccagcggc cttgcgcaag
     5341 ccctattagt acagacgtac ccaccgctgc cgacctggat ccagtccatc agccgggtct
     5401 gacactttct tgccgcgatc accacaggcc ctccagcgac tggccggccc gtccatcccg
     5461 ggatcaggcc ggtcccctgt ctgccgtccg ccggtcgtcg gcctcatgcg caccagggtt
     5521 ggagggaacc ccgtcgcgca tgccgcaaac ccggatcacc acgcaccgtg ggcccgcccc
     5581 gacagcccgg ccggcccaga accacgcgga cacccggcag gctgcccgac cgagcgcgcg
     5641 acacgctcga acagatgttt gaatcacgtt gttcgattct ctagggtggc ggcatggctg
     5701 gcaggaaccc attcgacaag acgccgcgac gcgggcgtcc gcccaagcag ccccccaaga
     5761 tgaccgtggg gcgcccgagc aatgccgagg tcgcgcagca gctggagatg gcgaagatca
     5821 cccagttgcc agacggcccc gctgataccg agggcctgac gccccggcag cgccgcatcc
     5881 tggagttgat ccacctcgcg gtgagcaccc gcggctaccc gccgacgatc cgcgagatcg
     5941 gcgaggctgc cggattggcc tccccgtcga gcgtgtcgca tcagttgcgc gctctcgagg
     6001 ccaagggcta cctgcgacgc gatccgcacc gtccacgagc catcgaggtg cggttccccg
     6061 agtcactctc cgcgatggag cctgactcca gcgaactggt cgctgacggc gtggtagagc
     6121 acgaacccga cgtcaccggc attggcgacg cgatgcccga ggcggtcaat gtgcccgtgg
     6181 tcggacgcat cgccgccggc ggcccgatcc tggcagagga gcgggtcgaa gacgtcttcc
     6241 cgttgcccaa gcagctggtc ggcgacggca cactgttcct gctcgaggtc aggggcgact
     6301 caatgatcga ggcggccatc tgcgacggtg acttcgtggt ggtgcggcag cagcccaccg
     6361 ccaacaacgg tgacatcgtg gccgccctgc tcgaagacga ggccaccgtg aagacgttca
     6421 agcgcaccga cggccaggtg tggttgatgc cacacaatcc ggcctactcg ccgatcgacg
     6481 gcaatcacgc gtcgattctg ggcaaggtgg tcgcggtgct gcgccgggtc taggtcgctc
     6541 gacgcagatg cgcgtctcga tccgggttcg cccaggcgca ggccgcacca gggtgggtgg
     6601 tcgctacggc gatgacattc tggtggtggc cgtg
//
LOCUS       DAOSUG010000111         6607 bp    DNA     linear   ENV 10-JUN-2024
DEFINITION  MAG TPA_asm: Micropruina sp. isolate AMR_MDS_2195
            NODE_5571_length_6607_cov_4.344628, whole genome shotgun sequence.
ACCESSION   DAOSUG010000111 DAOSUG010000000
VERSION     DAOSUG010000111.1
DBLINK      BioProject: PRJNA925477
            BioSample: SAMN32864262
            Sequence Read Archive: SRR4236673
KEYWORDS    WGS; ENV; Metagenome Assembled Genome; MAG; Third Party Data; TPA;
            TPA:assembly.
SOURCE      Micropruina sp. (wastewater metagenome)
  ORGANISM  Micropruina sp.
            Bacteria; Bacillati; Actinomycetota; Actinomycetes;
            Propionibacteriales; Nocardioidaceae; Micropruina.
REFERENCE   1  (bases 1 to 6607)
  AUTHORS   Abdulkadir,N., Saraiva,J.P., Zhang,J., Stolte,S., Gillor,O.,
            Harms,H. and Rocha,U.
  TITLE     Genome-centric analyses of 165 metagenomes show that mobile genetic
            elements are crucial for the transmission of antimicrobial
            resistance genes to pathogens in activated sludge and wastewater
  JOURNAL   Microbiol Spectr 12 (3), e0291823 (2024)
   PUBMED   38289113
REFERENCE   2  (bases 1 to 6607)
  AUTHORS   Abdulkadir,N.
  TITLE     Direct Submission
  JOURNAL   Submitted (24-MAR-2023) Environmental Microbiology, Helmholtz
            Center for Environmental Research -UFZ, Permoserstr 15, Leipzig
            04318, Germany
COMMENT     The annotation was added by the NCBI Prokaryotic Genome Annotation
            Pipeline (PGAP). Information about PGAP can be found here:
            https://www.ncbi.nlm.nih.gov/genome/annotation_prok/
            
            ##Genome-Assembly-Data-START##
            Assembly Method        :: SPAdes v. 3.11.1
            Genome Representation  :: Full
            Expected Final Version :: Yes
            Genome Coverage        :: Not Applicable
            Sequencing Technology  :: Illumina MiSeq
            ##Genome-Assembly-Data-END##
            
            ##Genome-Annotation-Data-START##
            Annotation Provider               :: NCBI
            Annotation Date                   :: 05/04/2023 04:57:10
            Annotation Pipeline               :: NCBI Prokaryotic Genome
                                                 Annotation Pipeline (PGAP)
            Annotation Method                 :: Best-placed reference protein
                                                 set; GeneMarkS-2+
            Annotation Software revision      :: 6.5
            Features Annotated                :: Gene; CDS; rRNA; tRNA; ncRNA
            Genes (total)                     :: 2,776
            CDSs (total)                      :: 2,750
            Genes (coding)                    :: 2,714
            CDSs (with protein)               :: 2,714
            Genes (RNA)                       :: 26
            tRNAs                             :: 24
            ncRNAs                            :: 2
            Pseudo Genes (total)              :: 36
            CDSs (without protein)            :: 36
            Pseudo Genes (ambiguous residues) :: 0 of 36
            Pseudo Genes (frameshifted)       :: 2 of 36
            Pseudo Genes (incomplete)         :: 32 of 36
            Pseudo Genes (internal stop)      :: 2 of 36
            ##Genome-Annotation-Data-END##
FEATURES             Location/Qualifiers
     source          1..6607
                     /organism="Micropruina sp."
                     /mol_type="genomic DNA"
                     /submitter_seqid="NODE_5571_length_6607_cov_4.344628"
                     /isolate="AMR_MDS_2195"
                     /isolation_source="Activated sludge of municipal
                     wastewater treatment plants"
                     /db_xref="taxon:2737536"
                     /environmental_sample
                     /geo_loc_name="Canada"
                     /lat_lon="49.25 N 123.1 W"
                     /collection_date="2015-06-16"
                     /metagenome_source="wastewater metagenome"
                     /note="metagenomic"
     gene            complement(<1..511)
                     /locus_tag="PLF56_05085"
     CDS             complement(<1..511)
                     /locus_tag="PLF56_05085"
                     /EC_number="2.-.-.-"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_020392711.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="GNAT family protein"
                     /protein_id="HOQ52969.1"
                     /translation="MNPARWPVELSDGPVVLRPWRLQDRRAWDELRQRNAAWTGPWDS
                     TRPPDSIEPPLTFAGMVRQFNRRARLGAMLPFAIDYRPGERQRSVLAGQLTISGITHG
                     SAGWAQAGYWVDQRWAGRGIIPTALALAADYCFFTLHLHRLEVAIRPENVRSLRVVEK
                     LGFRYEGSRP"
     gene            complement(519..1814)
                     /locus_tag="PLF56_05090"
     CDS             complement(519..1814)
                     /locus_tag="PLF56_05090"
                     /inference="COORDINATES: protein motif:HMM:NF015418.2"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_function: GO:0030151 - molybdenum ion binding [Evidence
                     IEA];
                     GO_function: GO:0046872 - metal ion binding [Evidence
                     IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="molybdopterin molybdotransferase MoeA"
                     /protein_id="HOQ52970.1"
                     /translation="MALFGRKRQSQPEGDQVDSAGLDVPPTAEPEPIGVAEYRAALLA
                     DIEPQQPIGLGVLDAFGRRLCENIVADLDLPTFTSAAVDGYAVHAAEVASASSGAPVR
                     LPVLDVLDSPVYRGAPLLPNSAVRVAAGAPLPEGADAVVPTEATDGGDVEVEVRASVA
                     EGQYVRRAGSDIANGTLLLAEGHVLDAGAVGLLAEVGHDKVLVRQPPRVVVLTVGSTL
                     VPPGLPLASRAHRYDATTMLISAAAKADGARVFPSGTFDDDAATLTRALGDLLILADL
                     VVIVGGLEGAVPQVLADLGEVHIHRVAINPGARQAFGRIGEDRTPVVVIPSGAASAFV
                     AYHAFVRPVLRTMQGEADASRVTRWLPARLALHGHDDATELIPAIVSERGVEPAGVPG
                     SELAYDVARADALIVLPPGTHLVPAHSDVEVWVLTPPAA"
     gene            1833..2465
                     /locus_tag="PLF56_05095"
     CDS             1833..2465
                     /locus_tag="PLF56_05095"
                     /EC_number="6.3.3.2"
                     /inference="COORDINATES: protein motif:HMM:TIGR02727.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_function: GO:0030272 - 5-formyltetrahydrofolate
                     cyclo-ligase activity [Evidence IEA];
                     GO_process: GO:0006730 - one-carbon metabolic process
                     [Evidence IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="5-formyltetrahydrofolate cyclo-ligase"
                     /protein_id="HOQ52971.1"
                     /translation="MPAAHRPNPPARASDVGFAKASLRAEIRQARRFAEPPTQREART
                     ASALRLSLPHRVIALYSSTGDEPDTWPLIDALAAAGRTVLLPVLGRRADGTVRHGPDW
                     AAYGGRHTLRVGFAGIAEPASGAQGPQALASATLIWCSALAASPGGERLGTGGGWYDR
                     ALAYAASDAVVGVLLREDEVRDRVPVEAFDRPIDLIVTGRRVIETTHATE"
     gene            2545..2892
                     /locus_tag="PLF56_05100"
     CDS             2545..2892
                     /locus_tag="PLF56_05100"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_004810823.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="zinc ribbon domain-containing protein"
                     /protein_id="HOQ52972.1"
                     /translation="MPTYQYRCLDCSNELEAVQKFTDDPLTECPDCDGTLRKVYSAVG
                     VVFKGSGFYATDNRSKGSAKSTTGSDAKGEGGAASAPAGGNGEKSTAGATGTTKPAAA
                     KATSKPAAGSPAA"
     gene            3061..3675
                     /locus_tag="PLF56_05105"
     CDS             3061..3675
                     /locus_tag="PLF56_05105"
                     /inference="COORDINATES: protein motif:HMM:NF020250.2"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="SAF domain-containing protein"
                     /protein_id="HOQ52973.1"
                     /translation="MKALLARLRRVVRTRRRLLAALLVGLAAWAALTALRPAPPPTVA
                     VLVAARAISGGVVIQSGDVVVRDLPQNVLPETYLTAADQAIGRPATLALPAGAVLLPA
                     SVVTRNSLASSGMAVLPVTLVDTAAGLIEVGDRIDLLGSSGDDTATVASAARVVAVLG
                     ADGSGSALTPSRSSGPVVLVELRPDALAKVAAAAAQGPLGFGFR"
     gene            3940..4311
                     /gene="mscL"
                     /locus_tag="PLF56_05110"
     CDS             3940..4311
                     /gene="mscL"
                     /locus_tag="PLF56_05110"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_016669254.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_function: GO:0008381 - mechanosensitive monoatomic ion
                     channel activity [Evidence IEA];
                     GO_process: GO:0006810 - transport [Evidence IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="large conductance mechanosensitive channel
                     protein MscL"
                     /protein_id="HOQ52974.1"
                     /translation="MKGFKDFLLRGNLIDIAVAFVIGGAFSAVTTSFTKVVVELLARL
                     GGAPNFDEWQPLGLVSAGPFLTALVAFVIMALVVYFGIVVPYEKVKALTTVAETETEA
                     GAPTTEELLIEIRDLLKQQQG"
     gene            complement(4468..4812)
                     /locus_tag="PLF56_05115"
     CDS             complement(4468..4812)
                     /locus_tag="PLF56_05115"
                     /inference="COORDINATES: ab initio
                     prediction:GeneMarkS-2+"
                     /note="Derived by automated computational analysis using
                     gene prediction method: GeneMarkS-2+."
                     /codon_start=1
                     /transl_table=11
                     /product="hypothetical protein"
                     /protein_id="HOQ52975.1"
                     /translation="MSEADLAAAVFCALTGQPSDSGGGDVHALLVEAGWDRAAITSHA
                     RAMLADGRVWPHPVPDALRLRVGSARLLAAVQREQQRLELFGEHAVPATRRALNADER
                     RLLQDVPPHHGS"
     gene            complement(4809..6182)
                     /locus_tag="PLF56_05120"
     CDS             complement(4809..6182)
                     /locus_tag="PLF56_05120"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_013865446.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="UTP--glucose-1-phosphate uridylyltransferase"
                     /protein_id="HOQ52976.1"
                     /translation="MSEQGLVASVAKMRAAGANEPAIEVFSHYYAEAAAGVTGMIPET
                     TIDPLTDPPQLSDVVIDEQTARDAIARTVIIKLNGGLGTSMGLDQAKTLLPVRDGLSF
                     LDLIVRQITAARATYDATLPLLFMNSFRTRDDTLAHLEKYPDLPVAGLPVDFLQNAEP
                     KITADTFEPVEWPADPTLEWCPPGHGDVYTALVGSGVLDALLAAGMKYASISNGDNLG
                     AAPDAELAGWFASSGAPYAAEICPRTVNDRKGGHLALRKSDGQLILRDTAQTPAEDMD
                     YFTDEFRHPYFHTNNLWIDLEQLKAALQQRGAVLGLPLIYNVKNVDPSDASSPKVIQL
                     ETAMGAAIEVFKGATAIAVPRSRFQPVKTTNELLLLRSDVFDLGNDSRLNQTVAEVPR
                     VDLDGHYYKLIDDFEARVTVPPSLRAATSFVVRGDWTFDRPVSAVGEVEYHDLGRPSV
                     AGVDDPA"
BASE COUNT         1043 a         2287 c         2257 g         1020 t
ORIGIN      
        1 ggggtcgtga cccttcgtag cgaaagccga gcttctcgac cacccgcagg ctacggacgt
       61 tctcgggccg gatggcgacc tcgagacgat gcaggtgcag ggtgaagaag cagtaatcgg
      121 cggccagcgc gagcgcggtc ggaatgatcc cgcggccggc ccagcgctgg tccacccagt
      181 accccgcctg cgcccagccg gccgaaccgt gggtgatacc cgagatggtg agctgtccgg
      241 ccagcacgga gcgctgacgc tcgccaggcc ggtagtcgat cgcgaacggc agcatggcgc
      301 cgagacgggc gcggcggttg aactgtcgca ccatgccggc gaaggtcagc gggggttcga
      361 tcgaatcggg cggacgggtc gaatcccagg ggcccgtcca cgcggcattg cgttgccgca
      421 gttcgtccca ggcgcgccgg tcctgcaacc gccacggacg cagcaccacg ggcccgtcgc
      481 tcaactcgac cggccagcgc gccgggttca tgccgcgctc atgcggccgg tggcgtcaac
      541 acccatacct cgacgtcgga gtgtgcagga accagatggg tgcccggcgg cagcacgatc
      601 agcgcgtccg cccgggccac gtcgtaggcg agctcggagc ccggcacccc ggcgggctcg
      661 accccccgct cagacacgat cgcaggaatg agttcggtgg cgtcgtcatg gccgtgcagg
      721 gccagccgcg ccggcagcca gcgagtgacc cgcgacgcgt ccgcctcgcc ctgcatggtg
      781 cgcagcaccg gccggacgaa cgcgtggtag gccacgaagg cggacgcggc cccgctcggg
      841 atcacgacca ccggggtgcg atcctcgccg atccggccga acgcctgcct tgcgcctggg
      901 ttgattgcca cccgatggat gtgcacctcg cccaggtcgg ccagcacctg cggcactgca
      961 ccctcgagac cgccgacgat caccacgagg tcggccagga tcaacaggtc gcccagcgcc
     1021 cgggtgagcg tggccgcatc gtcgtcgaac gtgcccgagg ggaagacccg ggccccgtcc
     1081 gccttggccg cggccgagat cagcatggtg gtggcgtcgt agcgatgcgc gcgcgacgcc
     1141 aggggtagcc ctggcggcac cagcgtggac ccgacggtga gcaccacgac ccgtggcggt
     1201 tgacgcacca gcaccttgtc gtgacccact tcggcgagca acccgacggc tccggcgtcc
     1261 agtacatgtc cctccgccag cagcagcgtg ccgttggcga tgtcggagcc cgcccggcgg
     1321 acgtactggc cctctgccac ggaggcgcgc acctcgacct cgacgtcgcc cccatcggtt
     1381 gcctcagtgg gcaccacggc atcggcaccc tcgggcagcg gcgcacccgc ggccacccgt
     1441 accgcactgt tcggcagcag gggtgcgccc cggtagacgg gcgagtcgag cacgtcgagc
     1501 acgggcagcc gcaccggcgc gccggacgac gccgaggcga cctcggcggc gtgcaccgcg
     1561 tacccatcga cggccgcgct ggtgaaggtg ggcaggtcga gatcggcgac gatgttctcg
     1621 cacagccggc ggccgaacgc gtccagcacc cccaggccga tcggctgctg cggttcgatg
     1681 tcggcgagca gcgccgcgcg gtactccgcc acgccgatcg gctcgggctc agcggtcgga
     1741 ggcacgtcca gccccgcaga atcgacctga tcgccctcgg gctgggactg ccgttttcgt
     1801 ccgaatagcg ccatggctct taccatagga caatgcctgc ggcccaccgt ccgaaccctc
     1861 cggcgcgggc gtcggatgtc ggtttcgcca aggcgtcgct gcgggccgag atccgccagg
     1921 ccaggcgctt cgccgagccc ccgacgcagc gtgaggctcg caccgcgagt gctctgcgac
     1981 tctcactacc gcaccgggtg atcgcgctct acagctccac cggcgacgag cccgacacct
     2041 ggccgctgat cgatgccctc gccgcagccg gacgcaccgt gctgctgccg gtgctcgggc
     2101 gtcgcgccga cggcaccgtg cgtcacgggc cggattgggc ggcctacggc ggacgccaca
     2161 cgctgcgcgt gggcttcgcc ggcatcgccg agcccgccag cggggcgcag ggaccgcagg
     2221 cgctggcttc ggccacgttg atctggtgtt cagcgttggc cgccagtcct ggcggcgaac
     2281 ggctcggcac cggcgggggc tggtacgacc gggccctggc atacgccgca tccgacgccg
     2341 tggtcggggt gctgctgcgt gaggacgagg tgcgcgaccg ggtgccggtc gaggccttcg
     2401 accggccgat cgacctgatc gtgaccggac gtcgcgtgat cgagacgacg cacgccacgg
     2461 aatagcgaac ccgctgtgcg gcgttgaatc ggcgaccgct agaatgcggc ggttgtccgc
     2521 cgctgttgca ccctggaaga gcccatgccc acgtatcagt accgttgcct ggattgttcg
     2581 aacgagctcg aagccgttca gaagttcacc gacgacccgc tgaccgaatg ccccgactgc
     2641 gacggcaccc tgcgcaaggt gtacagcgcc gtgggcgtcg tgttcaaggg ctccggcttc
     2701 tacgccaccg acaaccgctc gaagggatcg gccaagtcca ccaccggatc ggatgccaag
     2761 ggcgagggcg gcgccgcatc ggcgcccgcc ggcggcaacg gcgagaagtc gacggccggc
     2821 gccaccggca ccacgaagcc cgccgctgcc aaggccacga gcaagcctgc tgccgggtcg
     2881 ccggctgcct gacgtgtcgg cgcgtcgata cattcgcgac gtcggtcacc gcgcgatggc
     2941 acctgtgccc gctgaccagt aggtcgcctc gatacattcg cgacgtcggt caccgcgcgt
     3001 tcgcacctga cgcccgcgcg attgtgaaca cttcgccggt tccggtcggt tactgagggt
     3061 atgaaagcac tgttggcccg gttgcgccgg gtggtgcgca cccgtcgcag gttgctcgct
     3121 gcgctgctgg tcggcctggc cgcctgggca gcgctcaccg cgctacgacc ggcaccgccg
     3181 cccactgtcg ccgtgctggt ggcggcgcgc gcgatcagtg gcggggtggt gatccagtcc
     3241 ggcgacgtcg tcgtgcgcga cctgccgcag aacgtcctgc ccgagaccta cctgaccgct
     3301 gccgaccagg cgatcggtcg ccctgccacg ctggccctgc cggccggggc ggtgctgctg
     3361 cctgccagcg tggttactcg caactcactg gcctcctccg gcatggcggt gctgccggtc
     3421 accttggtcg acaccgcggc cggactgatc gaggtgggcg accgcatcga cctgctcggc
     3481 tcgtccggcg acgacactgc caccgtggca tcggcggcgc gggtggtcgc cgtgctgggc
     3541 gctgacggca gcggctcagc actgacgccg agccgttcca gcgggccggt cgtgctggtc
     3601 gaactgcgtc cggacgcgct ggcgaaggtc gcggcagcgg cggcgcaggg cccgctcggc
     3661 ttcgggttcc gctagggcgt accttctcgc gctggtcgat tccgtaagga cgtgccccgt
     3721 gcgtctgctg gcaaggcgca agacgatcca ccggttgaac tgtcgaaagc cgtgatcacc
     3781 agaacgctga cctgcgcacg atccgccctc cgggccactg tccacgaccc gagagcacgt
     3841 aatggccggg tcacggggtc cgactagatt cactcacctg cacagcccac caccgccctg
     3901 actcagaacg ggcggctgtg cgaaagggga atctacatga tcaagggatt caaggacttt
     3961 ctgctccgcg gcaacctgat cgacatcgcc gtggcgttcg tcatcggcgg cgcgttcagc
     4021 gccgtcacca cctcattcac caaggtcgtc gtcgagctgc tggcccggtt gggcggcgcc
     4081 ccgaacttcg acgagtggca accgctgggt ctggtgtccg ccgggccctt cctgaccgcg
     4141 ttggtggcct tcgtgatcat ggccttggtc gtctacttcg gcatcgtggt gccgtacgag
     4201 aaggtcaagg cgctcaccac ggtggccgag accgagaccg aggccggcgc gccgaccacc
     4261 gaagaactgc tgatcgagat ccgcgacctg ctgaagcagc agcagggctg accgtcggac
     4321 ggcgcctgcc cgaagtgcgg aattggtccc ccgtcgccat cggcggcggg ggaccaaggg
     4381 cagtgcggat cactgcgcga tccggtggtt gagcctgtcg aaaccccgcc catgagggtc
     4441 tcgacacgct aggtcagcgc ttctcgctca gctgccgtga tgcggcggaa cgtcctgcag
     4501 caaccgacgc tcgtcggcgt tcaaggcgcg tcgggtcgcg ggtaccgcat gctcgccgaa
     4561 cagttccagc cgctgctgct cgcgctgcac cgccgcgagt agccgggcac tgcccacccg
     4621 tagccgcagt gcgtccggta ccggatgcgg ccagactctc ccgtccgcaa gcatcgcacg
     4681 cgcgtggctg gtgatcgcgg cccgatccca gccggcctcc accagcaggg cgtgaacgtc
     4741 gccgccccca ctgtcgctgg gttggccggt gagcgcgcag aacaccgcgg cggccaggtc
     4801 ggcctcgctc acgccgggtc gtccacccct gccacgcttg gacggcctag gtcgtggtac
     4861 tcgacctcac ccacggcgct gaccggacga tcgaaggtcc aatcgccgcg cacgacgaac
     4921 gaagtggccg cccgcaacga cggcggcacc gtcacccgcg cctcgaagtc gtcgatcagc
     4981 ttgtagtagt ggccgtccag atcgacccga ggaacctcgg cgacggtctg gttgagtcgg
     5041 ctgtcgttgc cgaggtcgaa gacgtccgag cgcagcagca acagttcatt cgtggtcttc
     5101 accggctgga accgcgaccg cggcaccgcg atggcggttg ctcccttgaa cacctcgatg
     5161 gccgcaccca tggcggtctc gagctgaatc accttgggcg aggacgcgtc cgacggatcg
     5221 acgttcttga cgttgtagat caacggcaga ccgagcaccg ctccgcgctg ctgcagtgcg
     5281 gccttcagtt gctccaggtc gatccacagg ttgttggtgt gaaagtaggg gtggcgaaac
     5341 tcatcggtga agtagtccat gtcttcggcc ggcgtctgcg cggtgtcgcg caggatcagc
     5401 tgtccgtcgg acttgcgcag cgcgaggtga ccgcccttgc gatcgttgac ggtgcgcggg
     5461 cagatctctg ccgcgtacgg ggcaccggaa ctcgcgaacc agccggccag ttcggcatcg
     5521 ggcgcggcgc cgaggttgtc gccgttcgag atgctcgcat acttcatgcc agcggccagc
     5581 agcgcgtcca gcacgccgga cccgacgagc gcggtgtaga cgtcgccatg gccgggcggg
     5641 caccactcca aggtggggtc ggccggccat tccaccggct cgaaggtgtc ggcggtgatc
     5701 ttgggctcgg cattctgcag aaaatcgacc ggaagccccg ccaccggcag gtccgggtac
     5761 ttctcaagat gcgccagcgt gtcgtcccgg gtgcggaacg agttcatgaa cagcaacggc
     5821 agcgtcgcgt catacgtcgc acgcgcggcg gtgatctgcc tgacgatcag gtcgagaaag
     5881 gacagcccgt cacgaacggg cagcagggtc ttggcctggt cgagacccat ggacgtaccc
     5941 aggcccccgt tgagcttgat gatcaccgtg cgggcgatcg cgtcacgcgc cgtctgctcg
     6001 tcgatcacca cgtccgacag ttggggaggg tcggtgaggg ggtcgatggt ggtctcggga
     6061 atcatgccgg tgacgcctgc ggcggcctcg gcgtagtagt gcgagaagac ttcgatggcc
     6121 ggttcgttgg cacctgcggc acgcattttc gcgaccgagg caacgagtcc ttgttcactc
     6181 atgatattag tctaaatgac taatcgagtt acggaaagtc cagcgggcga ccacgacggg
     6241 gccaaggcct gatccacagc actcatctgc gtgaggacat gacgagagcg acgcctatcg
     6301 aagggcctaa gccgtctacc cacccccagg agacatcaac gagctggcgt cgctctctac
     6361 gagaaagcct agacgatacg tcagggaaaa gcacgtccga ttggtcatct ttttctagat
     6421 ccacaacttt tcaaccatcc ctgcgcgaag cccggaggcg ggtaccatgc cgggtctgac
     6481 cgcgcggcgc ggggccggga gtcgcagtat cgcgtgcccc tctatgctgt accttggccg
     6541 ctcggcccac gcgggcccca tagctcaggg gatagagcag aggtttccta aaccttgtgt
     6601 cgcaagt
//
LOCUS       DAOSUG010000112         6558 bp    DNA     linear   ENV 10-JUN-2024
DEFINITION  MAG TPA_asm: Micropruina sp. isolate AMR_MDS_2195
            NODE_5612_length_6558_cov_4.533600, whole genome shotgun sequence.
ACCESSION   DAOSUG010000112 DAOSUG010000000
VERSION     DAOSUG010000112.1
DBLINK      BioProject: PRJNA925477
            BioSample: SAMN32864262
            Sequence Read Archive: SRR4236673
KEYWORDS    WGS; ENV; Metagenome Assembled Genome; MAG; Third Party Data; TPA;
            TPA:assembly.
SOURCE      Micropruina sp. (wastewater metagenome)
  ORGANISM  Micropruina sp.
            Bacteria; Bacillati; Actinomycetota; Actinomycetes;
            Propionibacteriales; Nocardioidaceae; Micropruina.
REFERENCE   1  (bases 1 to 6558)
  AUTHORS   Abdulkadir,N., Saraiva,J.P., Zhang,J., Stolte,S., Gillor,O.,
            Harms,H. and Rocha,U.
  TITLE     Genome-centric analyses of 165 metagenomes show that mobile genetic
            elements are crucial for the transmission of antimicrobial
            resistance genes to pathogens in activated sludge and wastewater
  JOURNAL   Microbiol Spectr 12 (3), e0291823 (2024)
   PUBMED   38289113
REFERENCE   2  (bases 1 to 6558)
  AUTHORS   Abdulkadir,N.
  TITLE     Direct Submission
  JOURNAL   Submitted (24-MAR-2023) Environmental Microbiology, Helmholtz
            Center for Environmental Research -UFZ, Permoserstr 15, Leipzig
            04318, Germany
COMMENT     The annotation was added by the NCBI Prokaryotic Genome Annotation
            Pipeline (PGAP). Information about PGAP can be found here:
            https://www.ncbi.nlm.nih.gov/genome/annotation_prok/
            
            ##Genome-Assembly-Data-START##
            Assembly Method        :: SPAdes v. 3.11.1
            Genome Representation  :: Full
            Expected Final Version :: Yes
            Genome Coverage        :: Not Applicable
            Sequencing Technology  :: Illumina MiSeq
            ##Genome-Assembly-Data-END##
            
            ##Genome-Annotation-Data-START##
            Annotation Provider               :: NCBI
            Annotation Date                   :: 05/04/2023 04:57:10
            Annotation Pipeline               :: NCBI Prokaryotic Genome
                                                 Annotation Pipeline (PGAP)
            Annotation Method                 :: Best-placed reference protein
                                                 set; GeneMarkS-2+
            Annotation Software revision      :: 6.5
            Features Annotated                :: Gene; CDS; rRNA; tRNA; ncRNA
            Genes (total)                     :: 2,776
            CDSs (total)                      :: 2,750
            Genes (coding)                    :: 2,714
            CDSs (with protein)               :: 2,714
            Genes (RNA)                       :: 26
            tRNAs                             :: 24
            ncRNAs                            :: 2
            Pseudo Genes (total)              :: 36
            CDSs (without protein)            :: 36
            Pseudo Genes (ambiguous residues) :: 0 of 36
            Pseudo Genes (frameshifted)       :: 2 of 36
            Pseudo Genes (incomplete)         :: 32 of 36
            Pseudo Genes (internal stop)      :: 2 of 36
            ##Genome-Annotation-Data-END##
FEATURES             Location/Qualifiers
     source          1..6558
                     /organism="Micropruina sp."
                     /mol_type="genomic DNA"
                     /submitter_seqid="NODE_5612_length_6558_cov_4.533600"
                     /isolate="AMR_MDS_2195"
                     /isolation_source="Activated sludge of municipal
                     wastewater treatment plants"
                     /db_xref="taxon:2737536"
                     /environmental_sample
                     /geo_loc_name="Canada"
                     /lat_lon="49.25 N 123.1 W"
                     /collection_date="2015-06-16"
                     /metagenome_source="wastewater metagenome"
                     /note="metagenomic"
     gene            639..2087
                     /gene="gndA"
                     /locus_tag="PLF56_05125"
     CDS             639..2087
                     /gene="gndA"
                     /locus_tag="PLF56_05125"
                     /EC_number="1.1.1.44"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_009639639.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_function: GO:0004616 - phosphogluconate dehydrogenase
                     (decarboxylating) activity [Evidence IEA];
                     GO_function: GO:0050661 - NADP binding [Evidence IEA];
                     GO_process: GO:0006098 - pentose-phosphate shunt [Evidence
                     IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="NADP-dependent phosphogluconate dehydrogenase"
                     /protein_id="HOQ52977.1"
                     /translation="MSDKTGSAQIGVVGMAVMGSNLARNFASKGFSTAIFNRTYAKTE
                     AVLAAHPEAHFIGSEAIEDFVDSLEKPRRIIMMVKAGPATDATIDSLLPHLDQGDIIV
                     DGGNTYFPDTRRREKKLSDLGFNFIGCGISGGETGALLGPSMMPGGPKDSYAHIGPIL
                     EKVAAQYDGEPCCTWIGPDGAGHFVKMVHNGIEYADMQFIGEAYDLLKGVGLSAAEAA
                     DVFEDWNTGDLDSYLIEITSEVLRQVDAETGKPLVDVILDAAGMKGTGTWTVQESLNL
                     GVPAAAIAEAVFARAASSSPELRAAAQGALEGPDGTIQVDDRQQFIGDVRQALWCAKV
                     IAYAQGLHLIKVGGVEYGWDIDVAACARIWRAGCIIRAKLLDRISNEYAAGNLETLLE
                     APSISAELANSNAAWRRVVARASEAGVPIPGFSSALSYYDLVRAKRTNAALTQGLRDF
                     FGSHTYHRIDKAGTFHTEWSGDRSEVEADDAH"
     gene            2303..2800
                     /locus_tag="PLF56_05130"
     CDS             2303..2800
                     /locus_tag="PLF56_05130"
                     /inference="COORDINATES: ab initio
                     prediction:GeneMarkS-2+"
                     /note="Derived by automated computational analysis using
                     gene prediction method: GeneMarkS-2+."
                     /codon_start=1
                     /transl_table=11
                     /product="hypothetical protein"
                     /protein_id="HOQ52978.1"
                     /translation="MTVFLTLGVIGIVLLLTALLVGDHVDGALDALGGGEWFTGAALA
                     GFLGGVGFVGAAVLALTDSGWLAALAGLLAGGALGVAVGAITVRLRNSGEGGTPTSDA
                     LIGLPGTVVSDIPEHGYGTISLVNNGHLTRLNARAGIPLPAGTQVTVTEVLSPTSVMV
                     MPTYR"
     gene            2831..4321
                     /locus_tag="PLF56_05135"
     CDS             2831..4321
                     /locus_tag="PLF56_05135"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_016670041.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="SPFH domain-containing protein"
                     /protein_id="HOQ52979.1"
                     /translation="MNPLLIAIVGLVAILVLLGLLIVSRYKVAGPQEAYIVTGRRGKE
                     VRNAVTGAVSTDLSGQKVVMGGGVFVVPFVQRLHVLDLQSRRIPVQIRGAVSGQGVKL
                     NLDGVAIVKVGGNQDSVRAAAQRFLQQQDEVETFTQETLAGSLRAIIGGLSVEQIIRD
                     RAAFAQRVADEAESSLTGQGLVLDTFQIQDITDDGTYLADLGRPEAARIQQLAAIAEA
                     QARQAAEQSRIAAEQQIAISERELMLKRSEIKAETDAASAQAAASGPLAQADRDQAIL
                     TEQEKVAVRQAALKERQLDTEVRKPADAERYRVETDAAAKRQSAILTAEANKASAIAA
                     AEADAEKARLTGVGDKSRRAALAEAEAIEGARRGEAEKARRIAEAEALRAEGDAQAAA
                     ILATGEAEAEAMNKRADAYARYSEAAVLEMLVKVLPQVAREVAAPMANIDKLTVVSTD
                     GAGALPKQVNDNVVQTMELLKTTTGVDLTELLAKVTSKQVDTVRTS"
     gene            complement(4462..5331)
                     /locus_tag="PLF56_05140"
     CDS             complement(4462..5331)
                     /locus_tag="PLF56_05140"
                     /EC_number="3.1.2.-"
                     /inference="COORDINATES: protein motif:HMM:NF024675.2"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="thioesterase family protein"
                     /protein_id="HOQ52980.1"
                     /translation="MRPPAAIVRVPLRWVDLDAQGHVNNAVVADYLQEARVRFLLEGA
                     NAALLGNGTLVVGHQVEYLAPVEFANDPVEVRLWLGQVGASRFTIGYEVVQGEVIAAR
                     ARTVLCIFDFQAGGPRRMTHDERAWFVDRSGELPPLRELAKARPGPHAFRAPLTVRWT
                     DLDAYGHVNNVRFFDFVAEARVHMSSEADEAGNRMSAAAEAGHLWMVVRQDLDYLAQL
                     DHRLEPYETRVAVVDIGRTSMTLVAEVTDPRHGTVFARARTVIVCGDRAGRPIAVPDE
                     LRHGLETWPAVAG"
     gene            complement(5328..>6558)
                     /locus_tag="PLF56_05145"
     CDS             complement(5328..>6558)
                     /locus_tag="PLF56_05145"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_014846886.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=2
                     /transl_table=11
                     /product="AMP-binding protein"
                     /protein_id="HOQ52981.1"
                     /translation="RPKGVELAQRNWIYVGFGVDELDLIGADDLQYLWLPLSHVFGKC
                     LIAIQLAIGFTSAVDGRVEKIVHNLGITKPTFMCGAPRVFEKVRATVLSSNSTGLKGA
                     IARWSFTVGKKSHKQRLAGRPLGGLLALQYKVADRLVFTKLRERLGGRMRFMVSGSAK
                     LNAKVQGWFYSAGLVIIEGYGLTETTAVSFVDLPAHPHFGTVGRVLPGTEMKLGDDDE
                     VLLRGPGVMDAYHDAPELTAAAVIDGWFHTGDIGQLDGAGNLTLTDRKKDLMKTSGGK
                     YVAPQKVEGALTAAIPFVSQVIAVGDGRKYMSALITLDADNAKRWAAVNGMPHADYDE
                     IVRSEAAHQAVQHFIDRANGRLERWETVKRFTILPHELSVDDGLVTPSMKIRRANIDK
                     RFGAEIDAMYDKEPDDE"
BASE COUNT         1080 a         2297 c         2204 g          977 t
ORIGIN      
        1 ctggccacca tcgccggcat cgaagacctg accaacaacc tctcgggcaa gatctggacc
       61 aagatcagcc tgctgccctc ggctgtgcgg cgcacggcgg aacgtccgat cgatccggcg
      121 tgagctcatg ccgagccgcc cgggcacgta gtcgtgcggt gcgagctttc gtagccggcc
      181 agggagagcg aacgagggcc acagccgaag cgggtcctgg tcgcacatcg cccagtacct
      241 ggccggttgc ctgaaatccc tgcaccagtg acagacctcc acgcttggcg agcgtggacc
      301 cttcgcactg gtgcagggtt ttcagggcgc caggccagta ccgcgtcggc gatgtcgcct
      361 cgatgcggcc acgcgggctg ctcggtcggc ggcacctgcg atgcggaaga ccgaacaccc
      421 ctctcgatgc cgtctggcgg caataccacg gcgcgctgaa cgcgggccga gccctggccc
      481 ggccaacggc aacgggtggc aaccgcgggt gccgatcctc cgctcacgtg ttgactgctg
      541 gtcggagaag ctgtcccagc tctccgcgtg aactctccca aagcagtcag ggtttgagag
      601 aatggcgatg aaaagctctt tcgacagtga ggcagcagat gagcgacaaa accggtagcg
      661 cgcagatcgg cgtggtgggt atggcggtga tggggtcgaa cctggcccgc aacttcgcca
      721 gcaagggctt cagcaccgcc atcttcaacc gcacctacgc caagaccgag gccgtgctgg
      781 ccgcgcatcc ggaggcgcac ttcatcgggt ccgaggccat cgaggacttc gtcgactcgc
      841 tcgagaagcc gcgccgcatc atcatgatgg tcaaggccgg cccggccacc gacgccacca
      901 tcgactcgct gctgccgcac ctcgaccagg gcgacatcat cgtcgacggc ggcaacacct
      961 actttcccga cacccgccgg cgcgagaaga agctctccga cctgggcttc aacttcatcg
     1021 ggtgcggcat ctccggcggc gaaaccggcg ccctgctcgg gccgtcgatg atgcctggtg
     1081 gtcccaagga ctcctacgcc cacatcgggc cgatccttga gaaggtggcc gcccagtacg
     1141 acggtgagcc ctgctgcacc tggatcggcc cggacggcgc cggtcacttc gtcaagatgg
     1201 tgcacaacgg catcgagtac gcggacatgc agttcatcgg cgaggcctac gacctgctga
     1261 agggcgtcgg tctgtcggcg gccgaggcgg cggacgtgtt cgaggactgg aacaccggtg
     1321 acctcgactc gtacctgatc gagatcacct ccgaggtgct gcgtcaggtg gacgccgaga
     1381 ccggcaagcc gctggtcgac gtgattctgg acgctgccgg catgaagggc accggcacct
     1441 ggaccgtgca ggagtcgctc aacctgggcg ttcccgcggc ggcgatcgcc gaagcggtct
     1501 tcgcccgcgc cgcgtccagc agccccgaac tgcgggccgc cgcccagggc gcactggagg
     1561 gccccgacgg caccatccag gtggacgatc ggcagcagtt catcggcgac gtgcggcagg
     1621 cgctgtggtg cgccaaggtg atcgcctacg cgcagggcct gcacctgatc aaggtcggtg
     1681 gggtcgagta cggctgggac atcgacgtcg ccgcgtgtgc ccgcatctgg cgggcggggt
     1741 gcatcatccg cgccaaactg ctcgaccgca tctccaacga gtatgccgcc ggcaacctgg
     1801 agaccctgct ggaggcgccg tccatcagcg ccgaactggc caacagcaac gcagcctggc
     1861 gccgggtggt ggcccgggcc agcgaggccg gcgtgccgat tccgggtttc agctcggccc
     1921 tgtcgtacta cgacctggtg cgggccaagc gcacgaacgc cgccctcacc caggggctgc
     1981 gcgacttctt cgggtcgcac acctatcacc gcatcgacaa ggccggcacg ttccacaccg
     2041 agtggtcggg cgaccgctcc gaggtcgagg cggacgacgc gcactgaccc gtccgagatc
     2101 gaggcgcgca gcccactgac catccgctcg cctacctatc ctgcgctcgc caaccttggc
     2161 gagcgcagga tagttgggcg agcgttcgcg gcgccgccac cccggacgtc ccagcagggt
     2221 cggtccatcc cctcgtcgcc cacccacccc gacgcccggg ctggccccgt cgatcgttac
     2281 agttcggcgt gaagggagtt cagtgaccgt ctttctcacc ctcggagtga tcggcatcgt
     2341 gctcctgctc accgccctgc tcgtgggcga ccacgtcgac ggggccctgg acgcgctcgg
     2401 cggcggcgag tggttcaccg gcgcggctct ggccggcttt ctcggcggcg tcggcttcgt
     2461 cggggccgcg gttctggcgc tgaccgacag cggctggctc gccgcactcg ccgggctgct
     2521 cgccggcggt gcactcggtg tcgcggtcgg cgccatcacc gtgcggctgc gtaacagtgg
     2581 tgagggcggc accccgacgt ccgacgccct cattgggttg cccggcaccg tggtcagcga
     2641 cattcccgag cacggctacg gcacgatcag cctggtcaac aacggccacc tcacccggct
     2701 gaacgcccgg gccggcatac cgctgccggc cggcacccag gtcaccgtca ccgaagtgct
     2761 gtcgcccacc tccgtcatgg tcatgcccac ctaccgctga acccccacca acccgacccg
     2821 aaggaaaccc atgaacccgc tgttgatcgc catcgtcgga ctggtcgcga ttctcgtcct
     2881 cctcggcctg ctgatcgtca gccggtacaa ggtggccggc ccgcaggagg cttacatcgt
     2941 caccggacgt cgcggcaagg aggtgcgcaa cgcggtcacc ggcgcggtgt cgaccgatct
     3001 ctccggccag aaggtggtga tgggtggcgg cgtgttcgtg gtgccgttcg tccagcgtct
     3061 gcacgtgctc gacctgcaga gccggcgcat tccggtgcag atccgcggcg cggtctccgg
     3121 ccagggggtc aagctcaacc tggacggcgt cgccatcgtc aaggtgggcg gcaaccagga
     3181 ttcggtgcgg gccgccgccc agcgcttcct gcagcaacag gacgaggtgg agacgttcac
     3241 tcaggagacc ctggccggtt cgctgcgggc catcatcggc gggctgagcg tcgagcagat
     3301 catccgcgac cgagctgcct tcgcgcagcg ggtggccgac gaggcggagt cgtcgctgac
     3361 cggacagggg ctggtgctgg acaccttcca gattcaggac atcaccgacg acggcaccta
     3421 cctggccgac ctcggacgtc cggaggcggc ccgcatccag cagctggcgg cgatcgccga
     3481 agcgcaggcc cggcaggcgg cggaacagtc ccgcatcgcc gccgaacagc agatcgccat
     3541 ctccgaacgc gaactgatgc tgaagaggtc cgagatcaag gccgagacgg acgccgccag
     3601 cgcccaggcc gcggcgtccg gccccctggc gcaggccgac cgcgatcagg cgatcctcac
     3661 cgagcaggag aaggtcgcgg tgcgccaggc cgcgctgaag gaacggcagc tcgacaccga
     3721 ggtgcgcaag ccggcggacg cggagcgcta ccgggtcgag accgacgcgg ccgcgaagcg
     3781 tcagtcggcg atcctgaccg ccgaggccaa caaggcctcc gccatcgccg cggcggaggc
     3841 ggacgccgag aaggcccggc tgaccggtgt gggcgacaag tcgcgccggg ccgcgctggc
     3901 cgaggccgaa gccatcgagg gcgctcggcg cggtgaggcg gagaaggccc gccgaatcgc
     3961 cgaagcggag gcactgcggg cagagggcga cgcccaggcg gcggccattc tggccaccgg
     4021 cgaggcggag gccgaggcga tgaacaagcg cgcggacgcc tacgcccgct acagcgaggc
     4081 cgcggtgctg gagatgctgg tcaaggtgtt gccgcaggtg gcccgtgagg tggccgcgcc
     4141 gatggccaac atcgacaagc tgaccgtggt gtccaccgac ggtgccgggg ccttgccgaa
     4201 gcaagtcaac gacaacgtgg tgcagaccat ggagttgctg aagaccacca ccggcgtcga
     4261 tctcaccgaa ctgctcgcga aggtgaccag caagcaggtg gacacggtcc gcacgtcctg
     4321 accgccctgc gcgcacccat tccgctcgcc caactagctt ccgctcgcca accttggcga
     4381 gcggaaggta gttgggcgag cgtctgcggg gggcgcggca agcacggacc tgcaacctcc
     4441 cctgcattgg agcagacgcg atcagccggc gaccgcgggc caggtctcca acccgtgccg
     4501 cagctcgtcc ggcaccgcga tcggacggcc ggcccggtcg ccgcacacga tcaccgtgcg
     4561 ggcccgggcg aacacggtgc cgtgccgcgg gtcggtcacc tcggccacca gggtcatcga
     4621 ggtgcgtccg atgtcgacca ccgcgacgcg ggtttcatag ggctccagac ggtgatcgag
     4681 ttgggccagg tagtcgagat cctggcgcac caccatccac aggtgcccgg cctccgccgc
     4741 cgcgctcatg cggttgccgg cctcgtccgc ctcgctcgac atgtgcaccc gcgcctcagc
     4801 gacgaagtcg aagaaccgca cattgttgac gtggccgtag gcgtccagat cggtccagcg
     4861 gacggtcagc ggcgcccgga aggcgtgcgg gccgggacgc gccttggcca gctcgcgcag
     4921 cggcggcaac tcaccgctgc ggtcgacgaa ccacgcccgc tcatcgtgcg tcatgcgccg
     4981 cgggcctccg gcctggaaat cgaagatgca caacaccgtg cgggcgcggg ccgcgatcac
     5041 ctcgccttgg acgacctcgt acccgatggt gaaccgcgac gccccgacct gccccagcca
     5101 cagccgtact tcgaccggat cgttcgcgaa ctcgaccggt gccaggtatt cgacctgatg
     5161 accgaccacc agggtgccgt tgccgagcag ggcggcgttg gccccctcga gcagaaaccg
     5221 cacgcgcgcc tcctgcaggt agtcagcgac cacggcgttg ttgacgtgcc cctgcgcgtc
     5281 caggtcgacc cagcgcagcg gcacccgcac gatcgccgcg ggcggcctca ctcgtcgtcc
     5341 ggttctttgt cgtacatggc gtcgatctcg gcgccgaacc gcttgtcgat gttggcccgc
     5401 cggatcttca tgctcggcgt gaccagtccg tcatcgacgc tcaactcatg cggcagaatc
     5461 gtgaacctct tcaccgtctc ccaccgctcg agcctgccgt tggcccggtc gatgaagtgc
     5521 tgcacggcct ggtgcgccgc ctcgctgcgg acgatctcgt cgtagtcggc gtgcggcatg
     5581 ccgttgaccg ccgcccagcg tttggcgttg tcggcatcga gggtgatcag ggctgacatg
     5641 tacttgcggc cgtcgccgac cgcgatcacc tggctgacga acggtatggc agcggtcagg
     5701 gcgccctcca ccttctgggg tgccacgtac ttgccgccgg aggtcttcat caggtccttc
     5761 ttgcggtcgg tgagggtgag gttgccggca ccgtccaact gcccgatgtc gccggtgtgg
     5821 aaccagccgt cgatcactgc tgcggcggtc agctcggggg cgtcgtggta ggcgtccatg
     5881 acgccggggc cacgcagcag cacctcgtcg tcgtccccca gcttcatctc ggtgccgggc
     5941 agcacccgcc cgacggtgcc gaagtggggg tgggcgggca gatcgacgaa cgagaccgcc
     6001 gtggtttcgg tcagcccata gccttcgatg atcaccagtc cggcggaata gaaccagccc
     6061 tgcaccttgg cattcagctt ggccgagccc gacaccatga accgcatgcg tccaccgagc
     6121 cgctcgcgca acttggtgaa caccagccga tcggccacct tgtactgcag ggccaacagg
     6181 ccgcccaacg gccgcccggc caggcgctgc ttgtgactct tcttgcccac cgtgaacgac
     6241 cagcgggcga tcgccccctt gagaccggtg ctgttggacg acagcacagt ggcccgcacc
     6301 ttctcgaaga cgcgcggggc gccgcacatg aaggtgggct tggtgatgcc caggttgtgc
     6361 acgatcttct cgacgcgacc gtcgacggca gaagtaaaac cgatcgccaa ctggatcgcg
     6421 atcaagcact tgccgaaaac gtgcgacagc ggcagccaca ggtactgcag gtcgtcggcg
     6481 ccgatgaggt cgagctcatc gaccccgaag ccgacataga tccagttgcg ctgggccaac
     6541 tcgacgccct tgggacgt
//
LOCUS       DAOSUG010000113         6544 bp    DNA     linear   ENV 10-JUN-2024
DEFINITION  MAG TPA_asm: Micropruina sp. isolate AMR_MDS_2195
            NODE_5623_length_6544_cov_4.379565, whole genome shotgun sequence.
ACCESSION   DAOSUG010000113 DAOSUG010000000
VERSION     DAOSUG010000113.1
DBLINK      BioProject: PRJNA925477
            BioSample: SAMN32864262
            Sequence Read Archive: SRR4236673
KEYWORDS    WGS; ENV; Metagenome Assembled Genome; MAG; Third Party Data; TPA;
            TPA:assembly.
SOURCE      Micropruina sp. (wastewater metagenome)
  ORGANISM  Micropruina sp.
            Bacteria; Bacillati; Actinomycetota; Actinomycetes;
            Propionibacteriales; Nocardioidaceae; Micropruina.
REFERENCE   1  (bases 1 to 6544)
  AUTHORS   Abdulkadir,N., Saraiva,J.P., Zhang,J., Stolte,S., Gillor,O.,
            Harms,H. and Rocha,U.
  TITLE     Genome-centric analyses of 165 metagenomes show that mobile genetic
            elements are crucial for the transmission of antimicrobial
            resistance genes to pathogens in activated sludge and wastewater
  JOURNAL   Microbiol Spectr 12 (3), e0291823 (2024)
   PUBMED   38289113
REFERENCE   2  (bases 1 to 6544)
  AUTHORS   Abdulkadir,N.
  TITLE     Direct Submission
  JOURNAL   Submitted (24-MAR-2023) Environmental Microbiology, Helmholtz
            Center for Environmental Research -UFZ, Permoserstr 15, Leipzig
            04318, Germany
COMMENT     The annotation was added by the NCBI Prokaryotic Genome Annotation
            Pipeline (PGAP). Information about PGAP can be found here:
            https://www.ncbi.nlm.nih.gov/genome/annotation_prok/
            
            ##Genome-Assembly-Data-START##
            Assembly Method        :: SPAdes v. 3.11.1
            Genome Representation  :: Full
            Expected Final Version :: Yes
            Genome Coverage        :: Not Applicable
            Sequencing Technology  :: Illumina MiSeq
            ##Genome-Assembly-Data-END##
            
            ##Genome-Annotation-Data-START##
            Annotation Provider               :: NCBI
            Annotation Date                   :: 05/04/2023 04:57:10
            Annotation Pipeline               :: NCBI Prokaryotic Genome
                                                 Annotation Pipeline (PGAP)
            Annotation Method                 :: Best-placed reference protein
                                                 set; GeneMarkS-2+
            Annotation Software revision      :: 6.5
            Features Annotated                :: Gene; CDS; rRNA; tRNA; ncRNA
            Genes (total)                     :: 2,776
            CDSs (total)                      :: 2,750
            Genes (coding)                    :: 2,714
            CDSs (with protein)               :: 2,714
            Genes (RNA)                       :: 26
            tRNAs                             :: 24
            ncRNAs                            :: 2
            Pseudo Genes (total)              :: 36
            CDSs (without protein)            :: 36
            Pseudo Genes (ambiguous residues) :: 0 of 36
            Pseudo Genes (frameshifted)       :: 2 of 36
            Pseudo Genes (incomplete)         :: 32 of 36
            Pseudo Genes (internal stop)      :: 2 of 36
            ##Genome-Annotation-Data-END##
FEATURES             Location/Qualifiers
     source          1..6544
                     /organism="Micropruina sp."
                     /mol_type="genomic DNA"
                     /submitter_seqid="NODE_5623_length_6544_cov_4.379565"
                     /isolate="AMR_MDS_2195"
                     /isolation_source="Activated sludge of municipal
                     wastewater treatment plants"
                     /db_xref="taxon:2737536"
                     /environmental_sample
                     /geo_loc_name="Canada"
                     /lat_lon="49.25 N 123.1 W"
                     /collection_date="2015-06-16"
                     /metagenome_source="wastewater metagenome"
                     /note="metagenomic"
     gene            150..1403
                     /locus_tag="PLF56_05150"
     CDS             150..1403
                     /locus_tag="PLF56_05150"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_017934155.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="MFS transporter"
                     /protein_id="HOQ52982.1"
                     /translation="MPPEPDRPDTRGVHLLVVIGIVLLALNLRPAAVSVGPVLAEIVH
                     DLQLSGLEAGVLTSLPVLAFACFGALTPKLARMVGPHRLTLAALIAAAGGMFGRSLAT
                     DTWTFLLLTLVALSGMATANVLMPSLIKRHFPNRIGLMTAVYSTMLALGLTAASVLTV
                     PIAQHYGNWRHGLAAWGLMAAIAVLPWIGLIAHDRPDAAPSDRRQISLAAVARTRLGW
                     LMAVFFGMQSFHAYVIFGWFANLYREAGFSASDAGLLLGVITGISIPLSFLVPALSTR
                     QRLQLPMLGVLVGSYVAGYVGLLVAPQQGAVVWALLVGVGTSTFPLILTLIGLHAHTA
                     AGTAALSGFTQSVGYLIAATGPFLIGLLHDLTDGWTVPLIVLIALTVPLFWVGALVTR
                     PAAIEDQLPRTLRDDRPLASDEEGD"
     gene            complement(1408..1656)
                     /locus_tag="PLF56_05155"
     CDS             complement(1408..1656)
                     /locus_tag="PLF56_05155"
                     /inference="COORDINATES: ab initio
                     prediction:GeneMarkS-2+"
                     /note="Derived by automated computational analysis using
                     gene prediction method: GeneMarkS-2+."
                     /codon_start=1
                     /transl_table=11
                     /product="hypothetical protein"
                     /protein_id="HOQ52983.1"
                     /translation="MPITDGLPEPDALVAEGLVLAVAGVVVLAGVLRDGAGLGLADAA
                     GGVVLATGAGVDIGGAVAEQPVNSMTSTRQSPRGMPAA"
     gene            1646..2308
                     /locus_tag="PLF56_05160"
     CDS             1646..2308
                     /locus_tag="PLF56_05160"
                     /EC_number="3.5.1.28"
                     /inference="COORDINATES: protein motif:HMM:NF013670.2"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_function: GO:0008745 - N-acetylmuramoyl-L-alanine
                     amidase activity [Evidence IEA];
                     GO_process: GO:0009253 - peptidoglycan catabolic process
                     [Evidence IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="N-acetylmuramoyl-L-alanine amidase"
                     /protein_id="HOQ52984.1"
                     /translation="MIGKPLAGRTIVVDPGHNGVWTKALNRQVPAGNGRTKACNSSGT
                     AGGTFAEHAFTWATGQQLVARLRGLGAEVVLTRPNDTGSGPCVNVRAARTNAAHADLL
                     VSLHADGNLAKGARGYHIIVSSTMIGGAGVERRSLALARDLRARLDATGTMPRSTYIG
                     GGTAIHARTDIAGLNLIEVPGVMLELGNMRNATDLALQRSPAWRATVAKALAAGVVDA
                     LT"
     gene            2651..3943
                     /locus_tag="PLF56_05165"
     CDS             2651..3943
                     /locus_tag="PLF56_05165"
                     /inference="COORDINATES: protein motif:HMM:NF012475.2"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="glycoside hydrolase family 32 protein"
                     /protein_id="HOQ52985.1"
                     /translation="MSRQHSRPRWHYSPRSTWMNDPNGLIHYAGTWHLFYQSNPSDSS
                     WGNMSWGHATSTDLVTWTEHPVALWHTDTEQIYSGSCVFDATNSTGFGTAEWPPLVAI
                     YTAHYLPGSPRGGTEAQCLAYSLDGGSHWTTYAGNPVLDRGSANFRDPKAFRHGDSWV
                     MVAVEAEDQQVVIYRSPDLITWTHASTFGPAHAAAGAWECPDLFEVAVAGTDQTRWVL
                     LVNLNPGAVAGGSGCQYFVGDFDGHAFTPDRIIGTSVDRTLGLDDPLLRSYDWFDFGR
                     DCYAVVSFADAPHGRRVVIGWMNNWDYANQVPLTGGRSIMTIPRELDLVHLEDGYRLR
                     QRPVRELPADALVHHTLRSGDRISIQGVALEYHQGVLSLDRRGVGTDDFHEGFGTRQW
                     AEVPGEPGTVQVTVLVDTCSIEVFAAAGMVTISDLVFF"
     gene            4035..4835
                     /locus_tag="PLF56_05170"
     CDS             4035..4835
                     /locus_tag="PLF56_05170"
                     /inference="COORDINATES: protein motif:HMM:NF014625.2"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="PIG-L family deacetylase"
                     /protein_id="HOQ52986.1"
                     /translation="MSTIMFVHAHPDDEGTLTAGSIIRAADEGHTVVVVFATHGEHGE
                     APDDLAPGETVAQRRMAEAGRAAEVAGVARVHWLGYSDSGMAGWQQNDEPDAFVQADL
                     DEAAGRLVALLDQERPEVLVGYDWHGNYGHPDHIKVHQVVRRAAELTPGRPRLFEATM
                     NRDASRAQFEAAKAAGIAGANDFDPDAPMNDGNPLGTPEAEIALQVDVSDYLARRREV
                     MACHVSQVTDVQGMLALPEPAFAGMFSREHFIEVETGNPRPMRVGWFF"
     gene            complement(4901..5662)
                     /locus_tag="PLF56_05175"
     CDS             complement(4901..5662)
                     /locus_tag="PLF56_05175"
                     /EC_number="1.1.1.333"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_013862234.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_function: GO:0016491 - oxidoreductase activity
                     [Evidence IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="decaprenylphospho-beta-D-erythro-pentofuranosid-
                     2-ulose 2-reductase"
                     /protein_id="HOQ52987.1"
                     /translation="MFDAVGNPQSILLLGGTSEIGLAIISEYLRRQPVRVVLACQPGD
                     PLTEASMDALTAQGATAVEVLDFDATAFDTHPGVIEQAFAGGDIDLAIVAFGLLGDAE
                     ELWQNQAKAVQIAQVNYTGAVSVGVLLGERFKAQGHGQIVAMSTVAGERVRRSNFVYG
                     STKAGLDGFYLNLGEALDGLGPKVLVVRPGQVRTRMSAGVKEAPLTVDRQDAAKQIVQ
                     AIADGKTLIWVPPAFRFVMMALRHVPRPVFRKLPL"
     gene            complement(5707..>6544)
                     /locus_tag="PLF56_05180"
     CDS             complement(5707..>6544)
                     /locus_tag="PLF56_05180"
                     /inference="COORDINATES: protein motif:HMM:NF015963.2"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_component: GO:0016020 - membrane [Evidence IEA];
                     GO_function: GO:0003885 - D-arabinono-1,4-lactone oxidase
                     activity [Evidence IEA]"
                     /codon_start=2
                     /transl_table=11
                     /product="D-arabinono-1,4-lactone oxidase"
                     /protein_id="HOQ52988.1"
                     /translation="VKLKMTRTETAYFLADGVVTNSLDETIAVHSDGSESRYDYSSAW
                     FDAISAPPKLGRAALSRGNLATLAQLQEYAPKLAADPLAFHAKPLFELPDVFPNGLAN
                     KLTFSALGTVWYAKSGNYTNKIQTLTGFYHPLDMFSEWNRAYGPAGFLQYQFVVPPSA
                     IDEFKAIIGDIQRSGHYSFLNVFKLFGEGNRAPLSYPMAGWNVCVDFPIKPGLHEFLA
                     GLDARILEFGGRLYTAKDSRTSAATFHAMYPEIDSWIATRRAIDPDGVFVSDLARRLE
                     LI"
BASE COUNT          983 a         2265 c         2234 g         1062 t
ORIGIN      
        1 agcaattgct gctcgagcgt gtccagcccc aggcgaccag ttttttcgac acgctcaacc
       61 aaccgattgc tcgttgatca gcgcttcatt agctgatgtg tcaacaacat tctggttcgg
      121 tttcttcccc tacactcccg cgggtgacta tgccccccga gcctgaccgc cccgacaccc
      181 gcggcgtcca tctgctcgtg gtgatcggca tcgtgctgct cgcgctcaat cttcggcccg
      241 cggcggtgtc ggtgggaccg gtgctggccg agatcgtcca tgacctgcag ttgagcgggc
      301 tggaagcggg agttctgacc tcgctacccg tgctggcctt cgcctgtttc ggggcgttga
      361 cgcccaaact ggcccggatg gtggggccgc accggctcac cctggccgcc ctgatcgccg
      421 ccgccggtgg catgttcggc cgcagcctgg ccaccgacac ctggacgttc ctgctgctca
      481 ccctggtcgc gttgtcgggc atggcgaccg ccaatgtgct gatgccgtcg ctgatcaagc
      541 gccactttcc gaaccgcatc gggctgatga ccgccgtcta ttccacgatg ctggccctcg
      601 gcctgaccgc cgcctcggtg ctgaccgtgc cgatcgcgca gcactacggc aactggcgcc
      661 acgggctggc cgcctggggc ctgatggcgg ccatcgccgt gctgccgtgg atcggactga
      721 tcgcccacga ccgtccggac gccgccccgt ccgaccgtcg gcagatcagc ctggcggcgg
      781 tcgcccgcac ccgcctcggc tggttgatgg cggtgttctt cggcatgcag tcgtttcacg
      841 cgtacgtgat cttcggctgg ttcgccaacc tgtaccgcga ggccggcttc agcgcgtccg
      901 atgcggggtt gctgctgggc gtgatcaccg gcatcagcat tccgctgtcg tttctggtgc
      961 cggccctcag cacccgccag cggcttcagt tgccgatgct gggcgtgctc gtgggttcct
     1021 acgtcgccgg gtacgtcggg ctgttggtgg caccccagca gggtgcggtc gtctgggcgc
     1081 tgctggtcgg tgtgggcacc agcacgtttc cgttgatcct caccctgatc ggactgcatg
     1141 cccacaccgc ggcgggcacg gcggcgctgt cgggattcac ccagtcggtg gggtacctga
     1201 tcgccgcgac cggacccttt ttgatcggcc tgctgcacga cctgacggac ggctggacgg
     1261 tgccgctgat cgtgctgatc gccctgaccg tgccgttgtt ctgggtcggc gcgctggtca
     1321 ccaggcccgc cgccatcgag gatcagttgc cgcggacgct ccgcgacgac cggccgctgg
     1381 cgagcgacga agagggcgat tgacgtccta ggctgcaggc atgccccgcg gactctgcct
     1441 ggtgctggtc atgctgttga ccggctgttc tgccaccgcc ccgccgatgt cgaccccagc
     1501 acccgtggcg agcacgaccc cgccggctgc atcggccaag ccgagcccgg caccgtctcg
     1561 cagtacgccc gccaggacta ctacgcccgc cacggccagc actaagccct cggcgacgag
     1621 cgcgtcaggc tcgggcagcc cgtccgtgat cggcaagccc ctggccggac gcaccatcgt
     1681 ggtcgatccc ggccacaacg gcgtctggac gaaggcgctc aaccgtcagg tgccggccgg
     1741 caacggacgc accaaggcct gcaactcctc cggcaccgcc ggcggtacct tcgccgagca
     1801 cgccttcacc tgggcgaccg ggcagcaact ggtcgcgcgg cttcggggtc tcggcgccga
     1861 agtggtgctg accaggccga acgacaccgg cagtggtccc tgtgtcaatg tgcgggcggc
     1921 acgcacgaat gcggcgcacg ccgacctgct ggtatcgctg cacgccgacg gcaacctggc
     1981 caagggcgct cgcgggtatc acatcatcgt cagctcaacg atgatcggcg gcgccggcgt
     2041 ggagcgacgc tcgctcgctc tggcccgcga cctgcgcgcc cgcctggacg cgaccggcac
     2101 catgccgcgc tccacctata tcggcggcgg cacggcgatt catgccagaa ccgacatcgc
     2161 cgggctcaac ctgatcgagg tgcccggggt gatgctcgaa ctcggcaaca tgcgcaacgc
     2221 caccgacctg gccctgcaac gctcgccggc ctggcgcgcc accgtggcga aggcgctcgc
     2281 ggcaggagtg gtggacgcgc tcacctgagc cgggctcacc gaaggccggc gtcgcgagcg
     2341 gcaccagacg ggtgggctgg cgagccggtc cgcgtgaggg cacaccgggt cgcctggtga
     2401 gcgtgccggc gacgccaagg ggcccggatg tgggcgcgca gcagccggag atcgatggat
     2461 cctggctgag ccgggaggca cgcggcgatg gtcgtccggc tcgcgaggac cgggcctgcc
     2521 aagaccggga ctgccagccg tccatcgcac gcggcgcagc ggttcaaccg actcgaccgc
     2581 cccaacctgg cgagcgggtt ccaccgcctc ccgaggcccc ggcgtcgggt gacccagtac
     2641 gctgcgcacc atgtcgcgcc agcactcccg cccccgctgg cactactcgc cccgctcgac
     2701 ctggatgaac gaccccaacg ggctgattca ctacgccggc acgtggcacc tgttctatca
     2761 gtcgaacccg tctgattcga gctggggcaa catgtcgtgg ggacacgcca cgtccaccga
     2821 tctggtcacc tggaccgagc acccggtggc tctgtggcac accgacaccg agcagatcta
     2881 ttcgggcagt tgtgtgttcg atgcgaccaa ctcgacgggt ttcggcaccg ctgagtggcc
     2941 gccgctggtg gccatctaca ccgctcacta cctgcccggc agcccacgag gtggcaccga
     3001 ggcccagtgc ctggcctaca gcctggacgg cggcagccac tggaccacct acgccggcaa
     3061 cccggtgctc gaccgcggct cggccaactt tcgcgacccc aaggcgttcc ggcacggcga
     3121 ttcgtgggtg atggtcgccg tcgaggccga ggaccagcag gtggtgatct accgctcccc
     3181 cgacctgatc acctggacgc acgcctccac gttcggcccg gctcacgcgg ccgccggagc
     3241 ctgggaatgc cccgacctgt tcgaggttgc cgttgccggc acggatcaga cccgatgggt
     3301 gctgctggtc aacctgaacc cgggcgcggt ggccggcggc agcggctgcc agtacttcgt
     3361 cggagacttc gacggtcatg ccttcacccc cgaccgcatc atcggcacct cggtcgatcg
     3421 caccctcggc ctggacgatc cgttgctacg cagctacgac tggttcgatt tcggacggga
     3481 ctgctacgcg gtggtgtcgt tcgccgatgc accgcacgga cggcgcgtcg taatcggctg
     3541 gatgaacaac tgggactacg ccaaccaggt gccgctcacc ggcggccgca gcatcatgac
     3601 catcccccgc gaactcgacc tggtgcacct ggaggacggc taccggctgc ggcagcgtcc
     3661 ggtgcgcgag ttgccggcgg acgcattggt gcaccacacc ctgcgcagcg gcgaccggat
     3721 cagcattcag ggtgtggcgc ttgagtacca ccagggggta ctcagcctgg atcgccgcgg
     3781 ggtgggcacc gacgactttc acgaggggtt cggcacgcgg cagtgggccg aggtgcccgg
     3841 cgaacccggc acggtgcagg tgacggtact ggtcgacacg tgttcgatcg aggtgttcgc
     3901 cgcggcgggc atggtgacga tcagcgacct ggtgttcttc tgacccgagc gcccgcctaa
     3961 gtatctccag cctgcgaagc ttcgcgggcg cacgatactt aggtgggcgc tcggcggacg
     4021 gcataatggc ggccgtgtcc acgatcatgt ttgttcacgc tcaccccgac gacgagggca
     4081 ccctcaccgc cggctcgata atccgggccg cggacgaggg gcacaccgtc gtcgtggtct
     4141 tcgccaccca cggtgagcac ggagaggcac cggacgacct ggcgcccggc gagaccgtcg
     4201 cccagcgccg catggccgag gcgggccgcg ccgccgaggt ggcgggcgtg gcccgcgtcc
     4261 actggctggg gtattccgat tcgggcatgg ccggttggca gcagaacgat gagccggacg
     4321 ccttcgtgca ggccgacctc gacgaggcgg ccggacggct ggtcgccctg ctcgatcagg
     4381 agcgacctga ggtactggtc ggctacgact ggcacggcaa ctacgggcac cccgatcaca
     4441 tcaaggtgca ccaggtggtg cgccgcgcag ccgaactgac ccccgggcgt ccgcggctgt
     4501 tcgaagcgac gatgaaccgc gacgcctccc gcgcccagtt cgaggcggcc aaggcggccg
     4561 gaatcgcggg cgccaacgat ttcgatccgg acgcgccgat gaacgacggc aacccgctcg
     4621 gtactcccga ggccgagatc gcgctgcaag tggatgtcag cgactacctg gcccggcggc
     4681 gcgaggtgat ggcctgccac gtcagccagg tcaccgacgt gcagggcatg ttggcattac
     4741 ctgagccggc cttcgccggc atgttcagcc gtgagcactt catcgaggtc gagacgggca
     4801 acccgcggcc gatgcgggtc ggctggttct tctgagcggc gccctacgcc cggcccgcgc
     4861 tctgcgcgtg ggtcggcggc cggtcagccc cggcccacct tcagagcggc agcttgcgga
     4921 acaccggacg cggcacatgc cgcagcgcca tcatcacgaa ccggaaggcc ggcggcaccc
     4981 agatcagcgt cttgccatcg gcgatcgcct ggacgatctg cttggcggcg tcctgcctgt
     5041 caaccgtcag cggcgcctct ttgacaccgg ccgacatgcg ggtgcgcacc tggcccggac
     5101 gcaccaccaa caccttgggg cccagcccgt ccagcgcctc gccgaggttg aggtagaacc
     5161 cgtccaggcc cgccttggtc gagccgtaga cgaagttcga tcggcggacg cgctcgccgg
     5221 cgaccgtgct catcgccacg atctggccgt ggccctgcgc cttgaaccgc tcgccgagca
     5281 gcaccccgac gctgaccgcg ccggtgtagt tgacctgcgc gatctggacg gccttggcct
     5341 ggttctgcca cagttcttcg gcgtcgccga gcagcccgaa agccacgatc gcgaggtcga
     5401 tgtcaccgcc cgcgaaggcc tgctcgatca ccccggggtg ggtgtcgaaa gccgtggcgt
     5461 cgaagtcgag cacctcgacg gcggtggcgc cctgcgccgt caacgcgtcc atcgaggcct
     5521 cggtcagcgg gtcgcccggc tggcaggcca acaccacgcg gacgggctgt cgacgcaggt
     5581 attcgctgat gatggccagc ccgatctcgg aggtgcctcc cagcagcagg atggattgcg
     5641 ggttgccgac ggcgtcgaac atgggtctcg ctttcggtgc gtgagcgggg gtcagcggtg
     5701 ctcgggtcag atgagttcga ggcggcgggc caggtccgag acgaagacgc cgtcggggtc
     5761 gatggcccgc cgggtggcga tccaggagtc gatctcggga tacatcgcgt ggaaggtcgc
     5821 cgcgctggtc cggctgtcct tggccgtgta cagccgtccg ccgaactcga gaatgcgggc
     5881 gtccaggccg gccaggaact cgtggaggcc cggcttgatc ggaaagtcga cgcacacgtt
     5941 ccagcccgcc atcgggtagc tcagcggggc gcggttgccc tcgccgaaca gcttgaacac
     6001 gttcaggaac gagtagtggc cgctgcgctg aatgtcgccg atgatcgcct tgaactcgtc
     6061 gatcgcggac ggcggcacca cgaactggta ctgcagaaag ccggcgggac cgtaggcacg
     6121 gttccactcg ctgaacatgt ccagcgggtg gtagaagccg gtcagggtct ggatcttgtt
     6181 ggtgtagttg cccgacttgg cgtaccagac cgtgccgagt gccgagaacg tgagcttgtt
     6241 cgccagtccg ttggggaaca cgtccggcaa ctcgaacagc ggcttggcat gaaaggcgag
     6301 cgggtcggcg gccaacttgg gtgcgtactc ctgaagctgg gcgagggtgg ccaggttgcc
     6361 ccgcgacagg gcggcgcggc ccagcttcgg tggcgccgag atggcgtcga accacgcgga
     6421 gctgtagtcg tagcggctct cggagccgtc ggagtgcacc gcgatggtct cgtcgaggct
     6481 gttggtgacc acgccgtcgg cgagaaagta ggcggtctcg gtgcgggtca tcttcagctt
     6541 gacc
//
LOCUS       DAOSUG010000114         6534 bp    DNA     linear   ENV 10-JUN-2024
DEFINITION  MAG TPA_asm: Micropruina sp. isolate AMR_MDS_2195
            NODE_5635_length_6534_cov_4.148480, whole genome shotgun sequence.
ACCESSION   DAOSUG010000114 DAOSUG010000000
VERSION     DAOSUG010000114.1
DBLINK      BioProject: PRJNA925477
            BioSample: SAMN32864262
            Sequence Read Archive: SRR4236673
KEYWORDS    WGS; ENV; Metagenome Assembled Genome; MAG; Third Party Data; TPA;
            TPA:assembly.
SOURCE      Micropruina sp. (wastewater metagenome)
  ORGANISM  Micropruina sp.
            Bacteria; Bacillati; Actinomycetota; Actinomycetes;
            Propionibacteriales; Nocardioidaceae; Micropruina.
REFERENCE   1  (bases 1 to 6534)
  AUTHORS   Abdulkadir,N., Saraiva,J.P., Zhang,J., Stolte,S., Gillor,O.,
            Harms,H. and Rocha,U.
  TITLE     Genome-centric analyses of 165 metagenomes show that mobile genetic
            elements are crucial for the transmission of antimicrobial
            resistance genes to pathogens in activated sludge and wastewater
  JOURNAL   Microbiol Spectr 12 (3), e0291823 (2024)
   PUBMED   38289113
REFERENCE   2  (bases 1 to 6534)
  AUTHORS   Abdulkadir,N.
  TITLE     Direct Submission
  JOURNAL   Submitted (24-MAR-2023) Environmental Microbiology, Helmholtz
            Center for Environmental Research -UFZ, Permoserstr 15, Leipzig
            04318, Germany
COMMENT     The annotation was added by the NCBI Prokaryotic Genome Annotation
            Pipeline (PGAP). Information about PGAP can be found here:
            https://www.ncbi.nlm.nih.gov/genome/annotation_prok/
            
            ##Genome-Assembly-Data-START##
            Assembly Method        :: SPAdes v. 3.11.1
            Genome Representation  :: Full
            Expected Final Version :: Yes
            Genome Coverage        :: Not Applicable
            Sequencing Technology  :: Illumina MiSeq
            ##Genome-Assembly-Data-END##
            
            ##Genome-Annotation-Data-START##
            Annotation Provider               :: NCBI
            Annotation Date                   :: 05/04/2023 04:57:10
            Annotation Pipeline               :: NCBI Prokaryotic Genome
                                                 Annotation Pipeline (PGAP)
            Annotation Method                 :: Best-placed reference protein
                                                 set; GeneMarkS-2+
            Annotation Software revision      :: 6.5
            Features Annotated                :: Gene; CDS; rRNA; tRNA; ncRNA
            Genes (total)                     :: 2,776
            CDSs (total)                      :: 2,750
            Genes (coding)                    :: 2,714
            CDSs (with protein)               :: 2,714
            Genes (RNA)                       :: 26
            tRNAs                             :: 24
            ncRNAs                            :: 2
            Pseudo Genes (total)              :: 36
            CDSs (without protein)            :: 36
            Pseudo Genes (ambiguous residues) :: 0 of 36
            Pseudo Genes (frameshifted)       :: 2 of 36
            Pseudo Genes (incomplete)         :: 32 of 36
            Pseudo Genes (internal stop)      :: 2 of 36
            ##Genome-Annotation-Data-END##
FEATURES             Location/Qualifiers
     source          1..6534
                     /organism="Micropruina sp."
                     /mol_type="genomic DNA"
                     /submitter_seqid="NODE_5635_length_6534_cov_4.148480"
                     /isolate="AMR_MDS_2195"
                     /isolation_source="Activated sludge of municipal
                     wastewater treatment plants"
                     /db_xref="taxon:2737536"
                     /environmental_sample
                     /geo_loc_name="Canada"
                     /lat_lon="49.25 N 123.1 W"
                     /collection_date="2015-06-16"
                     /metagenome_source="wastewater metagenome"
                     /note="metagenomic"
     gene            <1..612
                     /locus_tag="PLF56_05185"
     CDS             <1..612
                     /locus_tag="PLF56_05185"
                     /inference="COORDINATES: protein motif:HMM:NF012334.2"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="SDR family NAD(P)-dependent oxidoreductase"
                     /protein_id="HOQ52989.1"
                     /translation="VDTLSADLARAEDVQQVADRVASTERPIDVLVNNAGYGLHVKLL
                     DDRPAEHRRAIDVMCVAVLLLGGAAGRAMKARGHGLIINVASLAAYISQGAYSPVKAY
                     VLAYSEGLANELHGTGVTVTAVNPGWVKTEFHQRAGIKTSSIPDFIWVDADRVARETL
                     ADAHKGRAISVPTKRWKLQKLFARHAPRGAVRWASRMLSSSRQ"
     gene            694..1530
                     /locus_tag="PLF56_05190"
     CDS             694..1530
                     /locus_tag="PLF56_05190"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_016666451.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="lysophospholipid acyltransferase family protein"
                     /protein_id="HOQ52990.1"
                     /translation="MSDDNTNRYSAAPGVAARFVSQKLVMKPYIWSILHVHEHGRRNL
                     DGLKPPFIAVANHSSHLDAPLVMGSLPRRLSRNLAAGAAADYFFDNWYTAAATRLFFN
                     AFPVDRKGLRNRKGLAGVLLSEGVPLLLFPEGTRSRTGAMKNFTPGHAALSISRNVPV
                     LPIALVGAYAAMPYGATVPVGGRPNVHVVFGRPLKAAPGEIAHQFAERIYRYVVELHD
                     STARAYGMPTQADFHRAVALRKAATEQAQLADAEAARGAEPPTDPDAQPRSDGAPETA
                     EG"
     gene            complement(1542..2273)
                     /locus_tag="PLF56_05195"
     CDS             complement(1542..2273)
                     /locus_tag="PLF56_05195"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_013864006.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_function: GO:0003677 - DNA binding [Evidence IEA];
                     GO_function: GO:0003700 - DNA-binding transcription factor
                     activity [Evidence IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="GntR family transcriptional regulator"
                     /protein_id="HOQ52991.1"
                     /translation="MTVREREPSAVRVPKYYWVKHEILALIADLAPGSPVPTERELAA
                     RFGTSRTTVRQAIAELVVEGRLVRTQGSGTFVAQPKLMVVRPLTSFSQDLQAEGWRPG
                     SVVLGISEEPASPEAAARLQIDVGAPVHRVERLRTAADEPIAHEVALLPGPLPDLAQQ
                     LAAHSSLYRTLHEAYGIDLATAEDTVETALADPVTAGLLQVDTGLPLLLINRTSWDAE
                     GRSVEWSIGRFRGDRFRFVSHQQLG"
     gene            2393..4474
                     /locus_tag="PLF56_05200"
     CDS             2393..4474
                     /locus_tag="PLF56_05200"
                     /inference="COORDINATES: protein motif:HMM:NF014437.2"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_process: GO:0009401 - phosphoenolpyruvate-dependent
                     sugar phosphotransferase system [Evidence IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="glucose PTS transporter subunit IIA"
                     /protein_id="HOQ52992.1"
                     /translation="MSTAATATSAKKSGPRIPGFAQLQRLGKSLMLPIALLPAAGILL
                     RLGQPDLMGQIKTPIIGPFFEAMSAAGGAIFGHLPLLFAIGVAIGFAKKADGSTALSA
                     AAGYLVLEAVFTTMSPVVLEGVTDQAGEQAMINYSVFGGILIGLITAWLFDRYHNIQL
                     PSYLGFFGGRRFVPIVTSLTSLFLGFGLSYFYPIFNSGLTAVGTFIGATGVVGAFVYG
                     FINRMLIPIGLHHIVNTYVWFIYGDYTTGAGKVVTGELTRFAAGDPTAGLLTSGFYPI
                     LMFGLPAAALAMIHVAKPKQKKVALGILSAAGLTAFLTGVTEPLEFAFMFVAFPLYVI
                     HAVLTGLSLAIAYALDIHLGFSFSAGLIDLLLYGTAPAAHNVPLLLVMGVVFFAIYYF
                     MFRFAITKWNMRTPGREDDADFEAEQAANLTDASAQGGPNAGSAKAEALIAAFGGREN
                     LVNVDACITRLRMEVADKSKVDQAKLKALGAAGVMEVGNNVQAIFGTQADALKSDINN
                     AIAASPTAPLEVAAAVDEVADAAPAGGSAVATAVATRVVAPVAGRPVALKNVPDATFA
                     QGIVGYGLAIDPPREVVEAVAPISGTVVKIWPHAYVIQNDDNTGVLVHLGLDTVQLDG
                     KGFTTHVADGDRVEAGQSMITYDVPAVEAAGRNPIIPVVVMDKKADAVSFTDAVIDEH
                     VNALQELFSVR"
     gene            4703..5488
                     /gene="nagB"
                     /locus_tag="PLF56_05205"
     CDS             4703..5488
                     /gene="nagB"
                     /locus_tag="PLF56_05205"
                     /EC_number="3.5.99.6"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_008354591.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_function: GO:0004342 - glucosamine-6-phosphate
                     deaminase activity [Evidence IEA];
                     GO_process: GO:0006046 - N-acetylglucosamine catabolic
                     process [Evidence IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="glucosamine-6-phosphate deaminase"
                     /protein_id="HOQ52993.1"
                     /translation="MEIVICPDAEQAGRLGADVIVKLLSRKPAAVLGLATGSSPLAIY
                     DELARRHEAGEVSFAKARGFSLDEYVGLAADHPETYHNVIRRDFVHRVDFDDSAVRGP
                     DGLAADIPAFCAEYEAAMAAAGGVDLQILGIGTDGHIGFNEPGSSLASRTRIKTLTKQ
                     TRVDNARFFDGDLDRVPTHCITQGLGTIMAARQILLVASGTGKAEAVHHMAEGSVSAM
                     WPGTILQHHPHVTVLLDEAAAGRLQLAGYYRETYAAKPAWQGL"
     gene            5515..>6534
                     /locus_tag="PLF56_05210"
     CDS             5515..>6534
                     /locus_tag="PLF56_05210"
                     /inference="COORDINATES: protein motif:HMM:NF014081.2"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_function: GO:0016787 - hydrolase activity [Evidence
                     IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="amidohydrolase family protein"
                     /protein_id="HOQ52994.1"
                     /translation="MLITADRVLTGTEVLSPGWIEVTDGVIAGIGGGVPDGGVDTALG
                     AVTVVPGFVDMHTHGGGSGAFPEATDQSSRAAVDLHRRHGTTTLIASLVSAHPAELLR
                     QASVLGEQVQAGLIAGIHLEGPWLSSLRCGAHEPSALRSPELSEIDRVFAAAKGGVRM
                     VTIAPELDGALPAIRRMVEWGAVAAVGHTDASYEQARAAVAAGATVATHLCNAMRPIH
                     HREPGPVVALMEDPRVTVELIVDGVHLHPALYRSVTHDAGPDRVALITDAMAAAGMPD
                     GSYTLGALDVDVTNGIAHVAGTGTIAGSTATMDRVFRNAVLASDRPRDEALALAVRQT
                     SLNPAR"
BASE COUNT         1003 a         2342 c         2173 g         1016 t
ORIGIN      
        1 gtcgacacgc tgagtgccga cctggcacgc gccgaggacg tgcagcaggt ggccgaccgc
       61 gtcgcgagca cggagcgccc gatcgacgtt ctggtcaaca acgcggggta cgggctgcac
      121 gtgaaactgc tcgacgaccg gcccgccgag catcgccgcg cgatcgacgt gatgtgcgtg
      181 gcggtgctgt tgctcggcgg agccgccggg cgggccatga aggcgcgcgg ccacgggctg
      241 atcatcaacg tggcgtcgct cgcggcctac atcagccagg gcgcgtactc gccggtcaag
      301 gcctacgtgc tggcctactc cgaaggcctc gccaacgaac tgcacggcac cggggtcacg
      361 gtgaccgcgg tcaaccccgg ctgggtgaag accgagtttc accaacgagc cggtatcaag
      421 acctcgtcca taccggattt catctgggtc gatgccgaca gggtcgcccg cgaaactttg
      481 gccgatgccc acaagggccg ggccatttcg gtgcccacca agcgctggaa gctacaaaag
      541 ctgttcgccc gtcacgcacc gcgcggcgct gttcggtggg cctcacgtat gttgagcagc
      601 agccgacagt gaacccgccg gcaccatgcc ccgcgcatgg cactagggtg tgctgcgcga
      661 ctcaagtgcg gatcgaccag cggaaggcga acggtgtctg acgacaacac caaccgttat
      721 tcagccgcgc cgggcgtggc cgctcgcttc gtctcgcaaa agttggtgat gaagccctac
      781 atctggtcga ttctgcacgt gcacgagcac ggccggcgca atctggacgg cctgaagccc
      841 cccttcatcg ccgtcgccaa ccattccagc cacctggacg ctccgctggt gatgggctcg
      901 ctcccccggc gcctgagccg caacctggcc gccggagcgg cggccgacta cttcttcgac
      961 aactggtaca ccgccgccgc cacccggctg ttcttcaacg cctttccggt cgatcgcaag
     1021 ggcctgcgca accgcaaggg gctggccggt gtgctgctca gcgagggtgt gccgctgctg
     1081 ctgttccccg aaggcacccg gtcgcgcacc ggcgcgatga agaacttcac cccggggcac
     1141 gccgccctct cgatcagccg caacgtgccg gtgctgccga tcgcgctggt cggcgcctac
     1201 gccgccatgc cctacggcgc caccgtgccg gtcggcgggc gtccgaacgt gcacgtggtg
     1261 ttcggacgtc cgctcaaggc cgctcccggc gagatcgcgc accagttcgc cgagcgcatc
     1321 taccgttatg tggtcgagtt gcacgacagc acggcccggg cctacggcat gcccacccag
     1381 gccgattttc accgggcggt ggcgctgcgc aaggcggcca ccgaacaggc ccagttggcg
     1441 gacgcggagg cggcccgcgg tgccgagccg cccaccgatc cggacgccca gccgcgctcc
     1501 gacggggctc ccgagaccgc agagggctga tccgggtcgc ctcagcccag ttgctggtgc
     1561 gacacgaagc ggaaccggtc gccgcggaac cgtccgatcg accattccac actgcgtccc
     1621 tctgcgtccc agctggtgcg gttgatcagc aacagaggca gcccggtgtc cacctgcagc
     1681 agtccggcgg tgaccgggtc ggccagcgcg gtctcgacgg tgtcttcagc ggtggccagg
     1741 tcgatcccgt aggcctcgtg cagggtgcgg tacaacgacg agtgggccgc gagttgctgg
     1801 gccagatcgg gcagcgggcc gggcagcagg gcgacctcgt gggcgatcgg ctcgtcggcg
     1861 gccgtgcgga gccgctccac ccggtgcacc ggggcgccga cgtcgatctg caaccgcgcg
     1921 gccgcctcgg gcgaggccgg ctcctctgag atgccgagca ccacactgcc cggacgccag
     1981 ccctcggcct gcaggtcctg gctgaacgag gtcagcggac gcaccaccat cagtttgggc
     2041 tgtgcgacga acgtgccgct gccctgcgtg cgcaccagcc gtccttccac gacgagttcg
     2101 gcgatcgcct gccgcacggt ggtgcgtgac gtgccgaagc gcgccgccag ctcacgctcg
     2161 gtcggcaccg gcgatccggg ggcgagatcg gcgatcagcg ccagaatctc gtgcttcacc
     2221 cagtagtact tgggcacccg cacggcgctc ggctcgcgtt ctcggacggt catggcacca
     2281 ccttacaaga ttggtctgga ccaaagcttg acgagggtca ttggtctatg ccaatctttt
     2341 cgcattggtc tataccaatc actgacagtc cgttccccac ccaaggagtg acatgagcac
     2401 cgccgccacc gcgacttcgg cgaagaagtc cggcccgcgc attcccggtt tcgcgcaact
     2461 gcagcggctc ggcaagagcc tgatgctgcc catcgccctg ctgccagccg ccggcattct
     2521 gctgcggctg ggccagcccg acctgatggg ccagatcaag accccgatca tcgggccgtt
     2581 cttcgaggcc atgagcgccg cgggcggcgc gatcttcggc cacctgccgc tgctgttcgc
     2641 gatcggcgtg gcgatcggct tcgccaagaa ggcggacggc tcgaccgccc tgtcggccgc
     2701 cgccggctac ctggtgctgg aggccgtgtt caccaccatg tcgccagtgg tgctggaggg
     2761 cgtcaccgat caggccggcg aacaggccat gatcaactac agcgtcttcg gcggcattct
     2821 gatcggcctg atcaccgcct ggctgttcga ccgctaccac aacattcaac tgccctcata
     2881 tctgggcttc ttcgggggac ggcgcttcgt acccatcgtc acctcgctga ccagcctgtt
     2941 cctcggcttc gggctcagct acttctaccc gatcttcaac tccggcctga ctgcggtcgg
     3001 caccttcatc ggcgccaccg gcgtggtcgg cgcgttcgtc tacgggttca tcaaccgcat
     3061 gctgattccg atcggcctgc accacatcgt caacacctac gtctggttca tctacggcga
     3121 ctacaccacc ggcgccggca aggtcgtcac tggtgagttg acccgcttcg ccgccggcga
     3181 cccgaccgcg gggctgctga cctcgggctt ctacccgatc ctgatgttcg gcctgccggc
     3241 cgccgcgctg gcgatgatcc acgtcgccaa gcccaagcag aagaaggtcg ccctgggcat
     3301 tctgtcggcc gccggcctga ccgcgttcct gaccggcgtc accgagccgc tcgagttcgc
     3361 cttcatgttc gtcgcgttcc cgctgtacgt gatccacgca gtgctgaccg gcctgtcgct
     3421 ggcgatcgcc tacgccctcg acattcacct cggcttctcg ttctcggccg gcctgatcga
     3481 cctgctgctc tacggcaccg ccccggcggc ccacaacgtg ccgctgctgc tcgtcatggg
     3541 cgtggtgttc ttcgcgatct actacttcat gttccggttc gcgatcacca agtggaacat
     3601 gcgcaccccc ggtcgtgagg acgacgccga cttcgaggcc gagcaggccg ccaacctgac
     3661 cgacgcgtca gcacagggcg ggccgaacgc cggcagcgcc aaggccgaag ccctgatcgc
     3721 cgccttcggt ggccgcgaga acctggtcaa cgtggacgcc tgcatcaccc ggttgcgcat
     3781 ggaggtcgcg gacaagtcca aggtcgacca ggccaagctc aaggcgctcg gcgcggccgg
     3841 cgtgatggag gtcggcaaca acgtgcaggc catcttcgga acccaggccg acgcgctgaa
     3901 gagcgacatc aacaacgcga tcgcggccag cccgacggca ccgctcgagg tcgccgccgc
     3961 cgtcgacgag gtggcggacg ccgctcccgc gggcggttct gcggtggcca ccgcggtcgc
     4021 gacgagggtc gtcgcgccgg tcgccggacg tccggtcgcg ctgaagaacg tgcccgatgc
     4081 caccttcgcc cagggcatcg tcggctacgg cctggccatc gacccgcctc gcgaggtggt
     4141 ggaggcggtc gccccgatca gcggcaccgt ggtcaagatc tggccgcacg cctacgtgat
     4201 ccagaacgac gacaacaccg gggtgctggt gcacctgggc ctggacaccg tgcaactcga
     4261 cggcaagggc ttcaccaccc acgtcgccga cggtgaccgg gtcgaggccg gccagtcgat
     4321 gatcacctac gatgtgcccg ccgtcgaggc ggccggtcgc aacccgatca ttccggtcgt
     4381 cgtgatggac aagaaggcgg atgcggtcag cttcaccgac gccgtgatcg acgagcacgt
     4441 gaacgccctg caggagctgt tcagcgtgcg ctgaccaggc ctgctagcgc ccatgcggct
     4501 ggcccgggcc catgtccccc gagcccgggc cggccgccct cccccaccat ccctccctcg
     4561 gctcatcaac agtctttcgc tggtcgagcg tcgagacccc gggcgaccgg gcgtttcgac
     4621 ggttcaccac cgagcgtgca atcgatcagc gcttgcttcg ccctcgctac cgttctttct
     4681 cacaccatta ggagtaccag tcatggaaat cgtcatctgc cccgacgccg agcaggccgg
     4741 ccggctcggc gccgacgtca tcgtcaagct gctcagccgc aagccggccg ctgtgctcgg
     4801 cctggccacc ggctcgtccc cgctggccat ctacgacgag ctggcccggc gccacgaggc
     4861 cggcgaggtg tcgttcgcca aggcccgcgg gttcagcctc gatgagtacg tcgggctggc
     4921 cgcggatcac ccggagacct accacaacgt gatccggcgc gacttcgtgc accgcgtcga
     4981 cttcgacgac agcgccgtcc gcggcccgga cggcctggcc gccgacattc ccgcgttctg
     5041 cgccgagtac gaggcggcga tggccgcggc cggcggggtc gacctacaga tcctcggcat
     5101 cggcaccgac ggccacatcg gcttcaacga gccgggctcg tcgttggcat cgcgcacccg
     5161 catcaagacg ctgaccaagc agacccgggt cgacaacgcc cggttcttcg acggtgacct
     5221 cgaccgggtg ccgacacact gcatcaccca gggcctgggc accatcatgg cggcccggca
     5281 gattctgctg gtggccagcg gcaccggcaa ggccgaggcc gtgcaccaca tggccgaggg
     5341 ctcggtgagc gccatgtggc cgggcacgat cctgcagcac cacccgcacg tcaccgtgct
     5401 gctggacgag gctgctgccg gccggctgca gctggccggc tactaccgcg agacctacgc
     5461 cgccaagccg gcgtggcagg gtctgtgagc gagcgaccgg acgggagcac gacgatgctg
     5521 atcacggctg atcgggtatt gaccggcacc gaggtgctga gcccgggctg gatcgaggtg
     5581 acggacgggg tgatcgccgg tatcggcggc ggcgtcccgg acggtggcgt cgacacggcg
     5641 ctgggtgccg tcaccgtggt gcccggtttc gtcgatatgc acacccacgg cggcggttcg
     5701 ggagcctttc cggaggccac cgaccagagc tcacgcgcgg ccgtcgatct gcaccgccgg
     5761 cacggcacca ccacgctgat cgcgtcgctg gtgtcggcgc atccggccga actgctgcgg
     5821 caggcgagcg tgctgggcga acaggtgcag gccggattga tcgccggcat tcacctggag
     5881 gggccgtggt tgtcgtcgct gcgctgtggc gcccacgagc cgtccgcgct gcgctcaccc
     5941 gagctgagcg agatcgaccg ggtgttcgcg gccgcgaagg gcggggtgcg catggtcacc
     6001 atcgctcccg agttggacgg tgcactgccc gcgatccggc gcatggtcga gtggggggcg
     6061 gtggccgcgg tcgggcacac cgatgccagc tacgagcagg cccgggcggc ggtcgcggcg
     6121 ggtgcgacgg tggcgacgca cctgtgcaac gcgatgcgtc cgatccatca ccgcgagccc
     6181 ggaccggtgg tggcgttgat ggaggatccc cgggtgaccg tcgaactgat cgtcgacggg
     6241 gtgcacctgc acccggcgct gtaccggtcg gtcacccacg acgcggggcc cgaccgggtc
     6301 gcgctgatca ccgacgcgat ggccgcggcg ggcatgcccg acggttccta caccctgggc
     6361 gccctcgacg tcgacgtgac caacggcatc gcacacgtgg caggcacggg caccatcgcc
     6421 ggcagcaccg ccaccatgga ccgggtgttc cgcaacgccg tgctcgccag cgaccggccg
     6481 cgcgacgagg cactggcgct ggccgtgcgg cagacctcgc tcaacccggc ccgg
//
LOCUS       DAOSUG010000115         6509 bp    DNA     linear   ENV 10-JUN-2024
DEFINITION  MAG TPA_asm: Micropruina sp. isolate AMR_MDS_2195
            NODE_5656_length_6509_cov_4.190889, whole genome shotgun sequence.
ACCESSION   DAOSUG010000115 DAOSUG010000000
VERSION     DAOSUG010000115.1
DBLINK      BioProject: PRJNA925477
            BioSample: SAMN32864262
            Sequence Read Archive: SRR4236673
KEYWORDS    WGS; ENV; Metagenome Assembled Genome; MAG; Third Party Data; TPA;
            TPA:assembly.
SOURCE      Micropruina sp. (wastewater metagenome)
  ORGANISM  Micropruina sp.
            Bacteria; Bacillati; Actinomycetota; Actinomycetes;
            Propionibacteriales; Nocardioidaceae; Micropruina.
REFERENCE   1  (bases 1 to 6509)
  AUTHORS   Abdulkadir,N., Saraiva,J.P., Zhang,J., Stolte,S., Gillor,O.,
            Harms,H. and Rocha,U.
  TITLE     Genome-centric analyses of 165 metagenomes show that mobile genetic
            elements are crucial for the transmission of antimicrobial
            resistance genes to pathogens in activated sludge and wastewater
  JOURNAL   Microbiol Spectr 12 (3), e0291823 (2024)
   PUBMED   38289113
REFERENCE   2  (bases 1 to 6509)
  AUTHORS   Abdulkadir,N.
  TITLE     Direct Submission
  JOURNAL   Submitted (24-MAR-2023) Environmental Microbiology, Helmholtz
            Center for Environmental Research -UFZ, Permoserstr 15, Leipzig
            04318, Germany
COMMENT     The annotation was added by the NCBI Prokaryotic Genome Annotation
            Pipeline (PGAP). Information about PGAP can be found here:
            https://www.ncbi.nlm.nih.gov/genome/annotation_prok/
            
            ##Genome-Assembly-Data-START##
            Assembly Method        :: SPAdes v. 3.11.1
            Genome Representation  :: Full
            Expected Final Version :: Yes
            Genome Coverage        :: Not Applicable
            Sequencing Technology  :: Illumina MiSeq
            ##Genome-Assembly-Data-END##
            
            ##Genome-Annotation-Data-START##
            Annotation Provider               :: NCBI
            Annotation Date                   :: 05/04/2023 04:57:10
            Annotation Pipeline               :: NCBI Prokaryotic Genome
                                                 Annotation Pipeline (PGAP)
            Annotation Method                 :: Best-placed reference protein
                                                 set; GeneMarkS-2+
            Annotation Software revision      :: 6.5
            Features Annotated                :: Gene; CDS; rRNA; tRNA; ncRNA
            Genes (total)                     :: 2,776
            CDSs (total)                      :: 2,750
            Genes (coding)                    :: 2,714
            CDSs (with protein)               :: 2,714
            Genes (RNA)                       :: 26
            tRNAs                             :: 24
            ncRNAs                            :: 2
            Pseudo Genes (total)              :: 36
            CDSs (without protein)            :: 36
            Pseudo Genes (ambiguous residues) :: 0 of 36
            Pseudo Genes (frameshifted)       :: 2 of 36
            Pseudo Genes (incomplete)         :: 32 of 36
            Pseudo Genes (internal stop)      :: 2 of 36
            ##Genome-Annotation-Data-END##
FEATURES             Location/Qualifiers
     source          1..6509
                     /organism="Micropruina sp."
                     /mol_type="genomic DNA"
                     /submitter_seqid="NODE_5656_length_6509_cov_4.190889"
                     /isolate="AMR_MDS_2195"
                     /isolation_source="Activated sludge of municipal
                     wastewater treatment plants"
                     /db_xref="taxon:2737536"
                     /environmental_sample
                     /geo_loc_name="Canada"
                     /lat_lon="49.25 N 123.1 W"
                     /collection_date="2015-06-16"
                     /metagenome_source="wastewater metagenome"
                     /note="metagenomic"
     gene            complement(<1..672)
                     /locus_tag="PLF56_05215"
     CDS             complement(<1..672)
                     /locus_tag="PLF56_05215"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_016670094.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="phenylalanine--tRNA ligase subunit alpha"
                     /protein_id="HOQ52995.1"
                     /translation="MSAQNPPQGADALRPEAIEASVTAALAAIAAADTMAELKDARLA
                     HAGDKSPLALANRAIGSIAPTERKEAGRLLGAARARVQQALAERTEQVEAAELARTLV
                     DEAVDVTLPIELQPQGAVHPITGLIDLICDEFVAMGWEVAEGPELEAEWLNFDALNIG
                     PDHPSRGEADTLFVEPVSNHLVMRTHTSPVQIRSLLERELPVYVVCPGKVYRADEYDA
                     THLPVF"
     gene            complement(769..1596)
                     /locus_tag="PLF56_05220"
     CDS             complement(769..1596)
                     /locus_tag="PLF56_05220"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_016670095.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_function: GO:0008173 - RNA methyltransferase activity
                     [Evidence IEA];
                     GO_function: GO:0016740 - transferase activity [Evidence
                     IEA];
                     GO_process: GO:0001510 - RNA methylation [Evidence IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="RNA methyltransferase"
                     /protein_id="HOQ52996.1"
                     /translation="MIPERLELPEPSQAVLRAARALLRRKERRATRRFLAEGPQAIRE
                     ALKLPGTVETVFFRWAAVHDHVELLSAARAADVPCYAVSEQNIATISDTVTPQGVVAV
                     CRALDLPLSDALADDPQLVVVCAQIRDPGNAGTVIRCADAFGADAVILSSDSVEVYNP
                     KTVRASVGSIFHLPVVTGVDLAEAIDACRAAGLQVFATDGEAGTELTDLTDRLARPTA
                     WVMGNESWGLPTEHLQLADTTVAVPIYGAAESLNLATAAAVCLYASASAQRTSTSQD"
     gene            complement(1801..2166)
                     /gene="rplT"
                     /locus_tag="PLF56_05225"
     CDS             complement(1801..2166)
                     /gene="rplT"
                     /locus_tag="PLF56_05225"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_016670096.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_component: GO:0005840 - ribosome [Evidence IEA];
                     GO_function: GO:0003735 - structural constituent of
                     ribosome [Evidence IEA];
                     GO_function: GO:0019843 - rRNA binding [Evidence IEA];
                     GO_process: GO:0006412 - translation [Evidence IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="50S ribosomal protein L20"
                     /protein_id="HOQ52997.1"
                     /translation="MARVKRSVNAQKKRREVLDQAAGYRGQRSRLYRKAKEQLLHSAT
                     YNYAHRKDRKGDFRSLWIQRINAAAREEGMTYNRFISGLKSAGVEVDRKMLAELAVND
                     APAFGKLVEVAKAGASVSA"
     gene            complement(2236..2442)
                     /gene="rpmI"
                     /locus_tag="PLF56_05230"
     CDS             complement(2236..2442)
                     /gene="rpmI"
                     /locus_tag="PLF56_05230"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_016670097.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_component: GO:0000315 - organellar large ribosomal
                     subunit [Evidence IEA];
                     GO_component: GO:0022625 - cytosolic large ribosomal
                     subunit [Evidence IEA];
                     GO_function: GO:0003735 - structural constituent of
                     ribosome [Evidence IEA];
                     GO_process: GO:0006412 - translation [Evidence IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="50S ribosomal protein L35"
                     /protein_id="HOQ52998.1"
                     /translation="MPKMKTHSGAKKRFRLTGTGKVMHRQAGKMHLNEHKASSRTRRL
                     DGDKVVAKSDAKKARKLLGSYKAR"
     gene            complement(2475..3218)
                     /gene="infC"
                     /locus_tag="PLF56_05235"
     CDS             complement(2475..3218)
                     /gene="infC"
                     /locus_tag="PLF56_05235"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_011755904.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_function: GO:0003743 - translation initiation factor
                     activity [Evidence IEA];
                     GO_process: GO:0006413 - translational initiation
                     [Evidence IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="translation initiation factor IF-3"
                     /protein_id="HOQ52999.1"
                     /translation="MHGRQHNATTQPLRPPGGKISEPRINERIHANEVRLVGPAGEQV
                     GIVRVEDALRLARESDLDLVEVAPMARPPVAKLMDYGKFKYEAAQKARESRRNQTNTV
                     IKEMKLRPKIDSHDYETKKGHVVRFLKAGDKVKITIMFRGREQSRPELGFNLLKKLAD
                     DVAEDGFVESAPKQDGRNMLMVLSPTRKKSEARVDAEAQKQVRMAERAAAAEAERTAE
                     AEHRASHQTESTTKRRRGPADNLDPDIDV"
     gene            complement(3735..4307)
                     /locus_tag="PLF56_05240"
     CDS             complement(3735..4307)
                     /locus_tag="PLF56_05240"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_015070785.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="SseB family protein"
                     /protein_id="HOQ53000.1"
                     /translation="MGAACQHRRAGYSTARVWPGCGWLGRVKTLGQPNAAWAGDRGEA
                     DAQVRAALRAAAAATGPDDYLAAVAALCGARLLMPIVAEGDDGGDGPVPDRHAELAAV
                     LMTSASGASGALAFSGLDALHAFNASARPVPCTLDEVAATAVEVGASAIVVDVAGPHL
                     LIIEGDLIGQLAAGRRLVRVPDGWGWLSVG"
     gene            4306..4746
                     /locus_tag="PLF56_05245"
     CDS             4306..4746
                     /locus_tag="PLF56_05245"
                     /inference="COORDINATES: ab initio
                     prediction:GeneMarkS-2+"
                     /note="Derived by automated computational analysis using
                     gene prediction method: GeneMarkS-2+."
                     /codon_start=1
                     /transl_table=11
                     /product="hypothetical protein"
                     /protein_id="HOQ53001.1"
                     /translation="MDLSTWVMIVVLAWTLVGLLLVAFIWTRAPWKAVLGWLGIALIP
                     LGVWLVGLSQAAVDGWNTLATWWQTLIFTPPVLIGLSVLGLAAALLVLSRLVPSRPRT
                     RRPKTPKTPTSTIPPAGGAPTYRQPPSSNPAEQTLILPENRPNP"
     assembly_gap    4882..4981
                     /estimated_length=100
                     /gap_type="within scaffold"
                     /linkage_evidence="paired-ends"
     gene            complement(<4982..5429)
                     /locus_tag="PLF56_05250"
     CDS             complement(<4982..5429)
                     /locus_tag="PLF56_05250"
                     /inference="COORDINATES: protein motif:HMM:NF022216.2"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="PH domain-containing protein"
                     /protein_id="HOQ53002.1"
                     /translation="MSVHEQPEPVTYRPKASLRMGVSLSVSLVAASLLGWFMTPPITR
                     ALFTPIQVLTLVFFLLIMVALALGIGLCYVRADAAGLRLRNGIKTYEVPWREVKAIRY
                     RDGDPWPFVLVRSEIEQRPLMGIQRSDRDYAERCVDDLRHRLAQAYG"
     gene            complement(5455..6300)
                     /gene="hisG"
                     /locus_tag="PLF56_05255"
     CDS             complement(5455..6300)
                     /gene="hisG"
                     /locus_tag="PLF56_05255"
                     /EC_number="2.4.2.17"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_015011627.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_function: GO:0003879 - ATP phosphoribosyltransferase
                     activity [Evidence IEA];
                     GO_process: GO:0000105 - histidine biosynthetic process
                     [Evidence IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="ATP phosphoribosyltransferase"
                     /protein_id="HOQ53003.1"
                     /translation="MLKIAVPNKGSLSEASTQMLREAGYRQRHDTKDLVLLDESNGVE
                     FYYLRPRDIAVYVGEGTLDLGITGRDLLIDSAAAAVEVLPLGFGGTRFRFAAPGGADW
                     TIADLQGKRIATSFPGLVRSFLDEQGLDARLIRLDGAVESSIRLGVADAIADVVETGS
                     TLKRAGLELFGDSIMTSEAILIQRAAAPAPEGLDRLIRRLTGVLTARSYYMVDYNVAE
                     AKLADACALTPGLDAPTVSPLAKPGWVAVRAMIPRQEAPQIMDRLYDVGADAILVTDI
                     VACRL"
     gene            complement(6330..>6509)
                     /gene="hisE"
                     /locus_tag="PLF56_05260"
     CDS             complement(6330..>6509)
                     /gene="hisE"
                     /locus_tag="PLF56_05260"
                     /EC_number="3.6.1.31"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_002527222.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_function: GO:0004636 - phosphoribosyl-ATP diphosphatase
                     activity [Evidence IEA];
                     GO_process: GO:0000105 - histidine biosynthetic process
                     [Evidence IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="phosphoribosyl-ATP diphosphatase"
                     /protein_id="HOQ53004.1"
                     /translation="DAGVHAIGKKIVEEAAEVWMAAEYQSRDDQALEISQLIYHTQVL
                     MIALGLDLDEVYAHL"
BASE COUNT          940 a         2210 c         2187 g         1072 t
ORIGIN      
        1 gaagaccggc aggtgggtgg cgtcgtactc gtcggcccgg tacaccttgc ccggacagac
       61 cacgtagacc ggcagctcgc gctccagcag cgaccggatc tgcaccggcg aggtgtgggt
      121 gcgcatcacc aggtggttgc tgaccggttc gacgaacagg gtgtcggcct cgccgcggct
      181 ggggtgatcg ggtccgatgt tgagcgcgtc gaagttgagc cattcggcct cgagttcggg
      241 cccctcggcc acctcccagc ccatcgcgac gaactcgtcg cagatcaggt cgatcagccc
      301 cgtgatcggg tggacggcgc cttgcggctg cagctcgatc ggcagcgtca cgtcgacggc
      361 ttcatcgacc agggtgcggg ccaactcggc cgcctcgacc tgctcggtcc gctcagccag
      421 cgcctgctgc acccgggcac gggcggcgcc cagcagccgt ccggcctctt tgcgctcggt
      481 cggcgcgatc gagccgatgg cacggttggc cagtgcgagc ggggacttgt cgccggcgtg
      541 ggcgagtcgt gcgtccttca gctcggccat cgtgtcggcc gcggcgatgg ccgccagtgc
      601 ggccgtcacc gaggcctcga tcgcttctgg acgcagggcg tccgcaccct gaggagggtt
      661 ctgcgctgac attggttctc cgttgtccgg cgccccgtgg acggggcggc tggtgccgcg
      721 ctggcgcggt tgttgcagtt gtggcgcccc tgatctggag cgtgtgcttc agtcctgcga
      781 ggtggacgtg cgttgcgcgc tggcgctcgc gtacaaacac acggcggcgg cggtggccag
      841 gttgaggctt tcggccgccc cgtagatcgg gacggcgacc gtggtgtcgg ccagctgcag
      901 atgctcggtc ggcaggcccc acgactcgtt gcccatcacc caggccgtcg gccgggccag
      961 ccgatccgtg aggtcggtga gctcggtgcc ggcctcaccg tcggtggcga acacctgcag
     1021 gccggcggcc cgacacgcgt cgatcgcctc ggccaggtcg accccggtga ccacgggcag
     1081 gtgaaagatg ctgcccacgc tggcgcggac cgtcttcggg ttgtacacct cgaccgagtc
     1141 ggacgacagg atcaccgcgt cggcgccgaa cgcgtccgca cagcggatca cggtgccggc
     1201 gttgcccggg tcacggatct gcgcgcagac caccaccagc tgcggatcgt ccgcgagcgc
     1261 gtccgacagc ggcaggtcga gcgcccggca gaccgccacg acgccctgcg gcgtgacggt
     1321 gtccgagatg gtggcgatgt tctgctcact gaccgcgtaa cacggcacgt cggcagcccg
     1381 tgccgcgctc agcagttcga cgtggtcgtg cacggcggcc cagcgaaaga acacggtctc
     1441 gacggtgccc ggcaacttga gcgcctcacg gatcgcctgc ggcccttcgg ccaggaaccg
     1501 gcgggtggcc cgtcgttcct tgcggcgcag caacgcgcgg gcggcgcgca gcaccgcctg
     1561 cgagggctct ggaagctcca gacgttccgg gatcatcgac gatcactttc gaagttcagg
     1621 acgggaccgc tccaggggaa cggcccgtca cgagggactg ctcggcggct cgcacgctcg
     1681 gtcggtgggg tgagccgggc ctgcctaccg gccactcgag cagggcggcc ggtgccgact
     1741 gctcggcgcg gcacggcgga tcgtgcgcaa tgccgcaccg ggacggtagc gggcctgcga
     1801 tcaggcgctg acgctcgcgc cggccttggc cacctcgacc agcttgccga acgcgggagc
     1861 gtcgttcacg gcgagttcgg ccagcatctt gcggtcgacc tcgacaccgg cggacttcag
     1921 gccgctgatg aagcggttgt aggtcatgcc ctcttcgcgg gccgcggcgt tgatgcgctg
     1981 gatccacagc gagcggaaat cgcccttgcg gtccttgcgg tgggcgtagt tgtaggtggc
     2041 gctgtggagc agttgctcct tcgccttgcg gtacaggcgc gaccgttggc cgcggtagcc
     2101 ggccgcctgg tcgagaactt cgcggcgctt cttctgtgcg ttcaccgaac gcttgacgcg
     2161 tgccatggtg gtactccttg tgttcagggt ccggaacggg ccggacctcg cgcgcgaggg
     2221 cgcgaatggt ctgggtcagc gggccttgta ggagccgagc agcttgcggg ccttcttggc
     2281 gtcgctcttg gccacgacct tgtcgccgtc gaggcggcga gtgcgcgagc tggccttgtg
     2341 ctcgttcagg tgcatcttgc cggcctggcg gtgcatcacc ttgccggtgc cggtgaggcg
     2401 gaaccgcttc ttggcaccgg agtgcgtctt catcttcggc atcgtgccgc tcgctttctt
     2461 gtttcttctc ggtcttacac gtcgatgtcg gggtcgagat tgtcggccgg accccggcgt
     2521 cgcttggtgg tggattcggt ctggtgcgag gcgcggtgct cggcctcggc ggtgcgctcg
     2581 gcctcggcgg ccgccgcccg ttcggccatg cgcacctgct tctgcgcttc ggcgtcgacg
     2641 cgggcctcgg acttcttgcg cgtcggcgac agcaccatca gcatgttgcg gccgtcctgc
     2701 ttgggcgcgg attcgacgaa gccgtcttcg gccacgtcgt cggccagctt cttcagcagg
     2761 ttgaagccga gttctggacg gctctgctcg cgaccccgga acatgatggt gatcttcacc
     2821 ttgtcgccgg ccttgagaaa gcggaccacg tgacccttct tggtctcgta atcgtggctg
     2881 tcgatcttgg gccgcaactt catttctttg atcacggtgt tggtctggtt gcgtcggctc
     2941 tcacgggcct tctgggcggc ctcgtacttg aacttgccgt agtccatgag cttggcgacc
     3001 ggcgggcggg ccatcggcgc cacctccacc aggtcgagat cggactcgcg ggcgagccgc
     3061 agggcgtcct cgacgcggac gatgccgacc tgttcgccgg caggcccgac caagcgcacc
     3121 tcgtttgcat ggatgcgctc gttgatccgt ggttcgctga tttttcctcc aggtggtcgc
     3181 agcggttggg ttgtggcgtt gtgctgccga ccatgcaaaa aggcctccgc ccaggcggag
     3241 gccccacgaa acgagccggc cgggcgcggt gcgcctggcc tggctggctg accctccgac
     3301 tcgaaggccg gacgggtggg agcggggctc cacttgcagc ggaccagccg gttggccggt
     3361 cgacgaacgt cattctagct tattgcctcg cggaccagcc aaatccgcct catcgttggt
     3421 tcagaccagc catcggttgt gcgtatcgag acccgatcgc cggtctgagc agccaaatcc
     3481 gcctcatcgt tggtccagac cagccatcgg tagagcgtat cgagacccga tcgctggtct
     3541 gagcagccaa atccgcctca tcgttggttc agaccaacca tcggttgagc gtatcgagac
     3601 ccgatcgccg gtctgacctg ccgaaacccg aacaccggtc aagcctgccg aggcccgatc
     3661 actggtcgag cctgtccaga cccgaacgcc ggtcgaggtt gtcgaaacct cttgggagca
     3721 tgtgttcgac acgctcaacc aaccgagagc cagccccacc cgtccggcac ccggacgagc
     3781 cgccgcccgg ccgccaactg gccgatcagg tcaccctcga tgatcagcag gtgcggaccg
     3841 gcgacgtcca ccacgatggc ggaggccccc acctcgacgg ctgtggccgc tacctcgtcc
     3901 aacgtgcagg gcacgggccg agcggaggcg ttgaaggcat gcaaggcgtc caggccgctg
     3961 aaggccagcg caccgctggc gcccgaggcc gacgtcatca acactgccgc cagttcggca
     4021 tgccgatcgg ggacgggacc gtcaccgccg tcgtccccct cggcgacgat gggcatcaac
     4081 agccgtgcgc cgcacaacgc ggcgactgcc gccagatagt cgtccgggcc ggtcgctgcg
     4141 gcggccgcgc gcagcgccgc gcgcacctgc gcatcggcct cgccgcgatc accggcccag
     4201 gcggcgttcg gctgccccaa tgtcttcacc cgacccagcc aaccacaccc cggccacacc
     4261 cgcgcggtgc tgtacccggc tcgccgatgc tggcaggctg cacccatgga tctttccacc
     4321 tgggtgatga tcgtcgtgct ggcctggacg ctcgtcggac tgctgctggt ggccttcatc
     4381 tggacccgcg cgccctggaa ggcagtgctc ggctggctgg gcatcgcact gatcccgctg
     4441 ggcgtctggc tggtcgggtt gtcgcaggcg gcggtggacg ggtggaacac gctcgccacg
     4501 tggtggcaga cgctgatctt caccccgccc gtgttgatcg ggctgagcgt tctgggcctg
     4561 gcggccgcgc tgctggtgct cagccgactc gtgccctcac gtccgcgcac ccgcaggccc
     4621 aagacgccca agacgccgac ctcgacgatt ccgccggccg gcggcgcgcc cacctatcgg
     4681 cagccgccgt cgagcaaccc ggccgagcag accctgatcc tgcccgagaa ccggcccaat
     4741 ccctgatcga gcggacaccc gggtctcacc gccctcgtcc ggctcgcgcc caatggctgc
     4801 gattcagccc tcggacgagt cgttctcgcc gtaggcctgt gccaatcggt gccgcagatc
     4861 gtccacgcaa cgctcggcat annnnnnnnn nnnnnnnnnn nnnnnnnnnn nnnnnnnnnn
     4921 nnnnnnnnnn nnnnnnnnnn nnnnnnnnnn nnnnnnnnnn nnnnnnnnnn nnnnnnnnnn
     4981 ncgccgtagg cctgtgccaa tcggtgccgc agatcgtcca cgcaacgctc ggcatagtcg
     5041 cggtcactgc gctgaatacc catcaacgga cgctgctcga tctcgctgcg caccagcacg
     5101 aacggccagg ggtcgccgtc gcggtaccgg atcgccttga cctcgcgcca gggcacctca
     5161 taggtcttga tgccattgcg cagccgaagc cccgcggcgt ccgcacgcac gtaacagagc
     5221 ccaatgccca gggccagcgc caccatgatc agcagaaaga acaccagcgt gagcacctgg
     5281 atcggggtga acagggcgcg ggtgatgggc ggcgtcatga accagccgag cagcgaggcc
     5341 gccaccaacg aaaccgacag cgacaccccc atgcgcagcg acgccttggg gcggtacgtc
     5401 accggttccg gctgctcgtg cacgctcatc gcgaacccat catcgaccgc gacctcacag
     5461 ccgacaggcg acgatgtcgg tgaccagaat cgcgtccgca cccacgtcgt agagccggtc
     5521 catgatctgg ggtgcctcct ggcgcggaat catcgcccgc accgccaccc agcccggctt
     5581 ggccaagggc gagacggtcg gcgcgtccag gccgggggtg agcgcgcacg cgtcagccaa
     5641 cttcgcctcg gcgacgttgt agtcgaccat gtagtagctg cgggcggtga gcacaccggt
     5701 gagccggcgg atcagccgat ccaggccctc gggcgccggc gcggcggcac gctggatcag
     5761 gatcgcctcc gaggtcatga tcgagtcgcc gaacaactcc aggcccgcgc gcttgagcgt
     5821 gctgccggtc tcgaccacgt cggcgatcgc gtccgcgaca ccgagccgaa tggacgattc
     5881 gacggcgccg tccagccgga tcaggcgggc atccaggccc tgctcgtcca agaaactgcg
     5941 aaccaggccg ggaaacgagg tggcgatgcg cttgccctgc aggtcggcga tcgtccagtc
     6001 ggcaccgccc ggggcggcga accggaatcg ggtgccgccg aagccgagcg gcagcacctc
     6061 gacggccgcg gcggccgagt cgatcagcag gtcgcggccg gtgatgccga ggtcgagggt
     6121 gccctcgccg acgtagacgg cgatgtcccg cgggcgcaga tagtagaact cgacaccgtt
     6181 gctctcatcg agcagcacca ggtccttggt gtcgtggcgc tgccggtagc cggcctcgcg
     6241 cagcatctgg gtggacgcct ccgaaaggga gcccttgttg ggcacggcga tcttcagcag
     6301 ggcaggctcg gacacttcag cgactctact cacaggtgcg cgtagacctc gtcgaggtcc
     6361 aagccgaggg cgatcatcag cacctgcgtg tggtagatca actggctgat ctcgagcgcc
     6421 tggtcatcgc gggactggta ctcggcggcc atccacacct cggcggcctc ttcgacgatc
     6481 ttcttgccga tggcgtgcac gccggcgtc
//
LOCUS       DAOSUG010000116         6458 bp    DNA     linear   ENV 10-JUN-2024
DEFINITION  MAG TPA_asm: Micropruina sp. isolate AMR_MDS_2195
            NODE_5683_length_6458_cov_4.198188, whole genome shotgun sequence.
ACCESSION   DAOSUG010000116 DAOSUG010000000
VERSION     DAOSUG010000116.1
DBLINK      BioProject: PRJNA925477
            BioSample: SAMN32864262
            Sequence Read Archive: SRR4236673
KEYWORDS    WGS; ENV; Metagenome Assembled Genome; MAG; Third Party Data; TPA;
            TPA:assembly.
SOURCE      Micropruina sp. (wastewater metagenome)
  ORGANISM  Micropruina sp.
            Bacteria; Bacillati; Actinomycetota; Actinomycetes;
            Propionibacteriales; Nocardioidaceae; Micropruina.
REFERENCE   1  (bases 1 to 6458)
  AUTHORS   Abdulkadir,N., Saraiva,J.P., Zhang,J., Stolte,S., Gillor,O.,
            Harms,H. and Rocha,U.
  TITLE     Genome-centric analyses of 165 metagenomes show that mobile genetic
            elements are crucial for the transmission of antimicrobial
            resistance genes to pathogens in activated sludge and wastewater
  JOURNAL   Microbiol Spectr 12 (3), e0291823 (2024)
   PUBMED   38289113
REFERENCE   2  (bases 1 to 6458)
  AUTHORS   Abdulkadir,N.
  TITLE     Direct Submission
  JOURNAL   Submitted (24-MAR-2023) Environmental Microbiology, Helmholtz
            Center for Environmental Research -UFZ, Permoserstr 15, Leipzig
            04318, Germany
COMMENT     The annotation was added by the NCBI Prokaryotic Genome Annotation
            Pipeline (PGAP). Information about PGAP can be found here:
            https://www.ncbi.nlm.nih.gov/genome/annotation_prok/
            
            ##Genome-Assembly-Data-START##
            Assembly Method        :: SPAdes v. 3.11.1
            Genome Representation  :: Full
            Expected Final Version :: Yes
            Genome Coverage        :: Not Applicable
            Sequencing Technology  :: Illumina MiSeq
            ##Genome-Assembly-Data-END##
            
            ##Genome-Annotation-Data-START##
            Annotation Provider               :: NCBI
            Annotation Date                   :: 05/04/2023 04:57:10
            Annotation Pipeline               :: NCBI Prokaryotic Genome
                                                 Annotation Pipeline (PGAP)
            Annotation Method                 :: Best-placed reference protein
                                                 set; GeneMarkS-2+
            Annotation Software revision      :: 6.5
            Features Annotated                :: Gene; CDS; rRNA; tRNA; ncRNA
            Genes (total)                     :: 2,776
            CDSs (total)                      :: 2,750
            Genes (coding)                    :: 2,714
            CDSs (with protein)               :: 2,714
            Genes (RNA)                       :: 26
            tRNAs                             :: 24
            ncRNAs                            :: 2
            Pseudo Genes (total)              :: 36
            CDSs (without protein)            :: 36
            Pseudo Genes (ambiguous residues) :: 0 of 36
            Pseudo Genes (frameshifted)       :: 2 of 36
            Pseudo Genes (incomplete)         :: 32 of 36
            Pseudo Genes (internal stop)      :: 2 of 36
            ##Genome-Annotation-Data-END##
FEATURES             Location/Qualifiers
     source          1..6458
                     /organism="Micropruina sp."
                     /mol_type="genomic DNA"
                     /submitter_seqid="NODE_5683_length_6458_cov_4.198188"
                     /isolate="AMR_MDS_2195"
                     /isolation_source="Activated sludge of municipal
                     wastewater treatment plants"
                     /db_xref="taxon:2737536"
                     /environmental_sample
                     /geo_loc_name="Canada"
                     /lat_lon="49.25 N 123.1 W"
                     /collection_date="2015-06-16"
                     /metagenome_source="wastewater metagenome"
                     /note="metagenomic"
     gene            complement(<1..2811)
                     /locus_tag="PLF56_05265"
     CDS             complement(<1..2811)
                     /locus_tag="PLF56_05265"
                     /inference="COORDINATES: protein motif:HMM:NF039608.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="hypothetical protein"
                     /protein_id="HOQ53005.1"
                     /translation="MRAAALALLVIGLSVGVVPNSASATPTPTPSESAAAAKPAEATP
                     TASAKASEAPSAEPTKSAGAEPSESPSPSASASPSADSTPTASASPSVVATGKPSTRG
                     GAVDPNAVPPVTNTTTVISVRVGGNRTGVTGVTAVQGVVLGLYATATSTTALQTCTSD
                     ADGDCNFTVANTGNGGANRNKQYYVKQISAPAGWLLNPALRTGDGDGANSQSTAYQFQ
                     TPELIGGQTISSQTTSNFMLRSGNTTRDASGGIWQVSKVNPTLPRSCGLDVALVLDLS
                     GSVTDVPALRGAADTLVNALQGTPSRMSLFSFSRVSPAINATQNYPALTSVSTAAQAA
                     DFKSRYAGWTAGGGTNWDRGLAVVANAADTPDLTVIITDGSPTFYNDPQEGPGTFTRF
                     RETENGIFSANALRSQGTRVIALGVGSGVSDTATGLNLAAISGPVRYNGTNSAVADYY
                     QSSSYAEAGQALRNLALGNCTGSISVTKQIVPSGNTGENVTGATPAGAGWVFNGSTST
                     AGIGGLPATRTTVADGTGAVNFPLTFPGGTTSAAVTLAETQQSGYTLVTQSTKNAVCT
                     NLLTGAAVTVTNSGATGFSINAPATAAVTCTVYNRAPQPPASIVVNKSWVINGTTYAE
                     GRQPDTIQAQLQLTGPGGAAATNQGWGVTRDGYTVGNTATISETVTLPALCTRGASTV
                     VRDGTTTPVSNLPYAASLSGTTNAFTVTNVVTCQSRLTLAKEVRGGTASPTAWTLQAV
                     APAGALTGPNGTSGSAGATNVLVTPGVTYQLRESGGPPEYAQIDNRTNLESNPLSTGS
                     MTCIEIDANGVQISGFSDGINGGVQVPIGSRVKCTAINQTAELRLAKEVVNDNGGTAV
                     PSDWTLTATPTGTFPAGLPVVTRPGAPIDSAQYVNVRPGVTYNLTESGLAGYQNTRLQ
                     CQIDGPATDATSITLRPLQSGTC"
     gene            3199..4434
                     /gene="xseA"
                     /locus_tag="PLF56_05270"
     CDS             3199..4434
                     /gene="xseA"
                     /locus_tag="PLF56_05270"
                     /EC_number="3.1.11.6"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_008358979.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_component: GO:0009318 - exodeoxyribonuclease VII
                     complex [Evidence IEA];
                     GO_function: GO:0008855 - exodeoxyribonuclease VII
                     activity [Evidence IEA];
                     GO_process: GO:0006308 - DNA catabolic process [Evidence
                     IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="exodeoxyribonuclease VII large subunit"
                     /protein_id="HOQ53006.1"
                     /translation="MPPLTSSPEQPQPLRVVLAGVKSWVERCGSVWVAGQLIELKRRS
                     GAATHFLTLRDALAEVSVTVTASTVVLDAAGPIAEGMQVVALVRPTVWTRNGSLSFEC
                     GDLRASGEGRLLAAIEQRKRMLQAEGLFDRARKRPLPFLPRGIGLITGQGSAAERDVL
                     QVAHRRWPAARFVVRHAVMQGQGCVDDVRDALSVLDRDPSVDVIVVARGGGSLQDLLP
                     FSDESLARAVFACGTPVVSAIGHEPDTPILDLVADVRAATPTDAAKRVVPDVTDERAR
                     VAQATERLRSGLLRMLDREAVHLQSLRSRPVLANPLGAVEVQADQVHQLHERARRAMR
                     ARVREESSWVDHTVNRVRALSPRATLLRGYSILSADDGGTINSAGMVTPGMQVRAMLA
                     DGTLHAQVTDVHMKEKADD"
     gene            4427..4687
                     /locus_tag="PLF56_05275"
     CDS             4427..4687
                     /locus_tag="PLF56_05275"
                     /EC_number="3.1.11.6"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_020385563.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_function: GO:0008855 - exodeoxyribonuclease VII
                     activity [Evidence IEA];
                     GO_process: GO:0006308 - DNA catabolic process [Evidence
                     IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="exodeoxyribonuclease VII small subunit"
                     /protein_id="HOQ53007.1"
                     /translation="MTDPIAGADAPQSGGPVAPPQSYEAARQRLVEVVQKLESGSVPL
                     SAAMDLWHEGESLARQCQQWLDGAKETIEQTLREDSSGRPTA"
     gene            5288..5446
                     /locus_tag="PLF56_05280"
     CDS             5288..5446
                     /locus_tag="PLF56_05280"
                     /inference="COORDINATES: ab initio
                     prediction:GeneMarkS-2+"
                     /note="Derived by automated computational analysis using
                     gene prediction method: GeneMarkS-2+."
                     /codon_start=1
                     /transl_table=11
                     /product="hypothetical protein"
                     /protein_id="HOQ53008.1"
                     /translation="MRHALVRLGVPDLPEIEALMIASPASAIESVLDGPDAHGGAPAY
                     LPIPPTPA"
     gene            5697..6044
                     /locus_tag="PLF56_05285"
     CDS             5697..6044
                     /locus_tag="PLF56_05285"
                     /inference="COORDINATES: ab initio
                     prediction:GeneMarkS-2+"
                     /note="Derived by automated computational analysis using
                     gene prediction method: GeneMarkS-2+."
                     /codon_start=1
                     /transl_table=11
                     /product="hypothetical protein"
                     /protein_id="HOQ53009.1"
                     /translation="MNTFTPFVVTVTITNGSGTAIDLVDMQVLDAPPAPDADPVHDPG
                     RVGPAPPAKVAAGKAATFRVALGVKRSGTLQVHVSLSPSRTRATFRPPTDRTRRHQAG
                     RAIPLGETCSRGV"
     gene            complement(6222..>6458)
                     /locus_tag="PLF56_05290"
     CDS             complement(6222..>6458)
                     /locus_tag="PLF56_05290"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_015582912.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="acetyl-coenzyme A synthetase"
                     /protein_id="HOQ53010.1"
                     /translation="KGQAIHAFVTLRVGAQGSQELAEDLRRHVAAHLSPIAKPDAIDF
                     PDKLPKTRSGKIMRRVLKARALGQDEGDLTTLED"
BASE COUNT          953 a         2117 c         2295 g         1093 t
ORIGIN      
        1 acaggtgccc gattgcaggg gtcgcagggt gatcgaggtg gcgtcggtcg cgggtccgtc
       61 gatctggcac tgcaggcggg tgttctggta acccgccagc ccggattcgg tcaggttgta
      121 cgtgactccg ggcctcacgt tcacgtactg ggcgctgtcg atcggtgctc ccggacgggt
      181 gacgaccggc agcccggccg ggaaggtgcc agtcggagtg gccgtcaggg tccagtcgct
      241 cggcacggct gtgccgccgt tgtcgttgac gacctccttg gccagccgca gctctgcggt
      301 ctggttgatc gcggtgcact tgacccggct gccgatcggc acttgcacgc cgccgttgat
      361 gccgtcggag aagcctgaaa tctgcacgcc gttcgcgtcg atctcgatgc aggtcatcga
      421 gccggtggag agcggattcg actccaggtt ggtgcggttg tcgatctggg cgtactccgg
      481 agggcccccg gactcgcgga gctggtacgt gaccccgggc gtcaccagca cgttcgtggc
      541 accggccgat ccggacgtgc cgttgggccc ggtcagagcc cccgccggcg cgaccgcctg
      601 cagcgtccag gcggtcggcg aggcggttcc accccggacc tccttcgcca gcgtcagccg
      661 gctctggcag gtcaccacgt tggtgaccgt gaaggcgttg gtggtgccac tcagggaggc
      721 ggcgtagggc aggttgctga ccggcgtggt ggtgccgtcg cgcaccacgg tcgacgcacc
      781 gcgcgtacac agggccggga gcgtcacggt ttcagagatg gtggccgtgt ttccgacggt
      841 gtagccgtca cgggtgacgc cccagccctg gttggtggct gcggcaccgc ccggtccggt
      901 caactgcagt tgggcctgga tggtgtccgg ctgccggccc tcggcgtagg tggtgccgtt
      961 gatcacccag ctcttgttca ccacgatcga tgccggcggc tgcggggcgc ggttgtacac
     1021 cgtgcaagtg accgcggcgg tggccggcgc attgatggag aaaccggtcg cgccgctgtt
     1081 ggtcaccgtg actgccgcgc cggtcagcag gttggtacag acagcgttct tcgtgctctg
     1141 ggtgaccagg gtgtagccgc tctgctgcgt ctcggccagg gtgaccgcag ccgaggtggt
     1201 tccgccgggg aaggtcagcg gaaagttcac cgcgccggtg ccgtctgcga cagtggtgcg
     1261 ggtggccggc agaccgccga tgccggccgt gctggtgctg ccgttgaaca cccagcccgc
     1321 cccggccggc gtggctccgg tcacgttctc gccggtgttg cccgacggca cgatctgctt
     1381 ggtgactgag atcgagccgg tgcagttgcc cagcgccagg ttgcgcagcg cctggcccgc
     1441 ctctgcgtag ctcgacgact ggtagtagtc ggcaaccgcg ctattggtgc cgttgtacct
     1501 cacggggccg gagatcgccg ccaggttgag gcccgtcgcg gtgtcgctga cgccgcttcc
     1561 gacgcccagt gcgatcaccc ttgtgccttg gctgcgcagc gcgtttgcgg agaagatgcc
     1621 gttttcggtc tcgcgaaaac gggtgaaggt gcccggacct tcttgcggat cgttgtaaaa
     1681 ggtcgggctg ccgtcggtga tgatcacggt gagatcaggg gtgtctgcgg cgttcgcgac
     1741 cacggccagg ccacggtccc agttggtgcc tccgccggcg gtccagccgg cgtagcgcga
     1801 cttgaagtcc gctgcctgcg cagcggtcga gaccgatgtg agcgcagggt agttctgggt
     1861 ggcgttgatg gccgggctga ccctcgaaaa gctgaacaac gacatccgcg acggggtgcc
     1921 ctgcagggcg ttcaccaagg tgtcggcggc gccacgcaga gccggcacgt ccgtcaccga
     1981 accggagaga tccaggacga gcgctacgtc caatccgcag gacctcggca gcgtggggtt
     2041 gaccttggac acctgccaga ttccgccgga ggcgtcgcgg gtggtgttgc cggagcgcag
     2101 catgaagttg ctggtggtct gcgacgagat ggtctggccg ccgatcagtt cgggggtctg
     2161 gaactggtag gccgtcgact gggagttggc gccgtcgccg tcgccggtgc gcagcgccgg
     2221 gttcagtagc cagccggcgg gcgccgagat ctgcttgacg tagtactgct tgttgcggtt
     2281 ggcgccgccg tttccggtgt tggcgacggt gaagttacag tcgccgtcgg cgtcggaggt
     2341 gcaggtctgc agggcggtag tggaggttgc agtcgcatag agcccgagca cgacgccctg
     2401 cactgcggtc acaccggtga cgccggttcg gttgccaccg acccggacgc tgatcacggt
     2461 ggtggtgttg gtcaccggtg gcaccgcatt cgggtccacc gcacccccgc gcgtcgacgg
     2521 cttgccggtt gccaccacag acggcgatgc cgaggccgtg ggagtggagt ccgcgctcgg
     2581 gctggcgctc gcgctcgggc tgggcgactc ggacggttct gcgccggcgg acttcgtcgg
     2641 ttcggcactc ggggcctcgg acgccttcgc ggaggcggtg ggcgtagcct cggcgggctt
     2701 ggcagctgcg gccgattcgg atggggtggg cgtcggggtc gcagaagcac tgttcggcac
     2761 cacaccgacg ctcagcccga tcaccagcag cgcgagggcc gcagcacgca ttctgaatcc
     2821 gaccgacgca acgcgcatga gaccccttct ccctggcgag ccgccccgca ccgcctgtcg
     2881 agactggagc agactaaccg aagctccggc agccgcgaca cctatcgagc acaaggttga
     2941 gacttgctaa gcaacgcgcg tgaatcacgg ggatcgcatc gatttgttat ggtcgtcggc
     3001 ccctgtctcg aacccaccga tacggccttt tgtcactgcc gctctcgtgc ctcgccgtgt
     3061 ggcggcgcct gggtgagtgg atccgaggtc gcgagggagg ggcagcaggt cgtctcgact
     3121 gaggagaacg cggcaggtgt gtgctcagat ggtctcgagt ggtgacccaa tcgggaggca
     3181 caccactagg gtgacggaat gccccccctg accagcagcc ccgagcagcc gcagccgctg
     3241 cgggtcgtgc tggccggggt caagagctgg gtcgagcggt gcggctcggt ctgggtcgcc
     3301 ggccagttga tcgagctgaa gcgtcgctca ggggccgcca cgcactttct caccctgcgt
     3361 gacgccctgg ccgaggtgtc ggtgacggtc acggcgtcga ccgtcgtgct ggatgccgca
     3421 ggcccgatcg ccgagggcat gcaggtggtg gcgctcgtcc ggcccaccgt gtggacgcgc
     3481 aacggctcgc tcagcttcga gtgcggcgac ctgcgcgcgt ccggcgaagg acggctgttg
     3541 gccgccatcg agcagcgcaa gcgcatgttg caggctgaag gactcttcga tcgggcccgc
     3601 aagcggccgc tgccctttct gccccgaggg atcggcctga tcaccgggca gggcagtgcc
     3661 gccgaacgcg acgtgctgca ggtggcgcac cgccggtggc ccgccgcgcg gttcgttgtg
     3721 cggcatgcgg tgatgcaggg ccaaggctgt gtggacgacg tgcgcgacgc gctcagcgtg
     3781 ctcgaccggg atccgtccgt cgacgtgatc gtggtggcca gaggaggcgg ttcgctgcag
     3841 gatctgctcc cgttctcgga cgagtccctg gctcgggccg tcttcgcctg cggcacgccc
     3901 gtggtcagcg ccatcggtca cgagcctgac accccgatcc tcgatctggt cgccgacgtg
     3961 cgtgccgcca cccccaccga cgccgccaaa cgggtggtgc ccgatgtgac ggacgagcgg
     4021 gcgcgggtgg cgcaggcgac cgaacgactg cgcagcggcc tgttgcgcat gctcgaccgg
     4081 gaggccgtcc acttacagtc acttcgctcc agaccggtgc tggcgaaccc actgggcgcg
     4141 gtcgaggtgc aggccgatca ggtgcaccag ctgcacgagc gggcgcgtcg agccatgcgg
     4201 gccagggtgc gcgaagagtc gtcgtgggtc gatcacaccg tgaaccgcgt gcgcgcgctg
     4261 tcccccaggg ccacactgct tcgcggctac agcattctga gcgccgacga cggtggcacc
     4321 atcaactccg caggcatggt gacgccgggg atgcaggtgc gtgcgatgct cgcggacggc
     4381 accctgcacg cgcaggtcac cgacgtgcac atgaaagaga aggcagatga ctgatccaat
     4441 cgccggtgcc gacgctccgc agtccggcgg gcccgtggcg ccaccgcagt cctacgaagc
     4501 cgcacggcaa cgcctggtcg aggtcgtcca gaagctggag tcaggctcgg tgcccctgtc
     4561 ggcggccatg gatctctggc acgagggcga gtcgctggcc aggcagtgcc agcagtggct
     4621 cgacggcgcc aaagagacca tcgagcagac cctgcgcgag gattcctccg gccgaccgac
     4681 tgcataggtt caaaagatct ttgcgttcgc tgtcggcgta gcgtcaaaac tgtggcttaa
     4741 gtcagtaaag gcgcaagatt ctgctatatt agtgggagca actaacaatt agtacaggag
     4801 gtgctgccga tgagcacgat cgaccgctgg ctggcctccc tcaggctgat gcaggtgcag
     4861 gacatgcgcg tctaccgcga cctgcgctga gcagcccgag cgagttccag aaaagctcca
     4921 ggccctttcg tccgatggac gaaagggcct tcgtggctac gtccgggcgc tacggtcgtc
     4981 accgcaccga ggggcccacg acgattcagg agatgacgaa gtcggtgtcg gttcaggcgc
     5041 tcgcgcgtct actcgcgttg ccggacatgt atgcgggaat gatctcgaac gacggcccga
     5101 cgtttgccgg atcggacgcg atcgtggccg actccgatca cgcggtgctc attcattgca
     5161 cggccggcaa agaccgcacg ggcgttgccg tggcccttct gctggacgcg gtcggcaccg
     5221 atcgagcgtc cctggtggcc gactacgcgg cctcgcacga gcgacagtcc gggtctgggc
     5281 ggacggcatg cggcacgcgc ttgtccgtct gggtgttccg gacctgcccg agatcgaggc
     5341 gctgatgatc gcttcgccgg cctcggccat cgaatccgtg ctcgacggcc cggacgcgca
     5401 cggcggtgcg cccgcctacc tgccgattcc cccaaccccc gcctaactat ctcccgcccg
     5461 cgaagcttcg caggcgctgg atggttaggc gggggttcgg gattcgccgc acggcggtag
     5521 cgtcggctcc gagaagttca tcgaccaggc cggccactac ggcggcttga cgcctgtcct
     5581 cgagcgccgc gaccaccacc ccgtcccgct tcggcaccac ccaccgctgg ccggacggag
     5641 tcaccgtcac ggtgaccaag ccagcgccct gctggcccag cggatgggtt cgcgaggtga
     5701 acaccttcac acccttcgtg gtgacggtga ccatcacgaa cggaagcggt acggccatcg
     5761 acctggtcga catgcaggtg ctggacgctc cgccggcgcc ggacgccgat ccggtgcacg
     5821 accccggcag ggtcggcccc gcaccaccgg cgaaggtggc cgccggcaag gcggcgacgt
     5881 ttcgagtggc cctgggcgtg aagaggtccg gcacgctgca ggtgcacgtg agcctgtccc
     5941 ccagccgcac ccgcgccacc ttccgccccc cgaccgatcg aacacgtcgg caccaggctg
     6001 gtcgagctat tccgctgggc gaaacgtgca gccgcggggt ctgaggttct gccgatgcgt
     6061 cgacaccagt ccccgtggtt gacctcgcgc ggccaggtac tcatcacgca cgggcgaacc
     6121 cgctcaacca gccctacgac cccgcagcga tgctgcagct ctgcggacgg cgtctccaca
     6181 cgctcgaccg gcagtccggt ttcgaccgat gcgccgagaa ctcagtcctc cagcgtggtg
     6241 aggtcgccct cgtcctgacc cagcgcacgc gccttcagca cccggcgcat gatcttgccc
     6301 gagcgggtct tgggcagttt gtcgggaaag tcgatcgcat cgggtttggc gatcggcgac
     6361 aggtgcgctg ccacgtggcg gcgcagatcc tcggccagtt cctggctgcc ctgagcgccg
     6421 acccggagcg tgacgaaggc atggatcgcc tgcccctt
//
LOCUS       DAOSUG010000117         6450 bp    DNA     linear   ENV 10-JUN-2024
DEFINITION  MAG TPA_asm: Micropruina sp. isolate AMR_MDS_2195
            NODE_5689_length_6450_cov_4.006880, whole genome shotgun sequence.
ACCESSION   DAOSUG010000117 DAOSUG010000000
VERSION     DAOSUG010000117.1
DBLINK      BioProject: PRJNA925477
            BioSample: SAMN32864262
            Sequence Read Archive: SRR4236673
KEYWORDS    WGS; ENV; Metagenome Assembled Genome; MAG; Third Party Data; TPA;
            TPA:assembly.
SOURCE      Micropruina sp. (wastewater metagenome)
  ORGANISM  Micropruina sp.
            Bacteria; Bacillati; Actinomycetota; Actinomycetes;
            Propionibacteriales; Nocardioidaceae; Micropruina.
REFERENCE   1  (bases 1 to 6450)
  AUTHORS   Abdulkadir,N., Saraiva,J.P., Zhang,J., Stolte,S., Gillor,O.,
            Harms,H. and Rocha,U.
  TITLE     Genome-centric analyses of 165 metagenomes show that mobile genetic
            elements are crucial for the transmission of antimicrobial
            resistance genes to pathogens in activated sludge and wastewater
  JOURNAL   Microbiol Spectr 12 (3), e0291823 (2024)
   PUBMED   38289113
REFERENCE   2  (bases 1 to 6450)
  AUTHORS   Abdulkadir,N.
  TITLE     Direct Submission
  JOURNAL   Submitted (24-MAR-2023) Environmental Microbiology, Helmholtz
            Center for Environmental Research -UFZ, Permoserstr 15, Leipzig
            04318, Germany
COMMENT     The annotation was added by the NCBI Prokaryotic Genome Annotation
            Pipeline (PGAP). Information about PGAP can be found here:
            https://www.ncbi.nlm.nih.gov/genome/annotation_prok/
            
            ##Genome-Assembly-Data-START##
            Assembly Method        :: SPAdes v. 3.11.1
            Genome Representation  :: Full
            Expected Final Version :: Yes
            Genome Coverage        :: Not Applicable
            Sequencing Technology  :: Illumina MiSeq
            ##Genome-Assembly-Data-END##
            
            ##Genome-Annotation-Data-START##
            Annotation Provider               :: NCBI
            Annotation Date                   :: 05/04/2023 04:57:10
            Annotation Pipeline               :: NCBI Prokaryotic Genome
                                                 Annotation Pipeline (PGAP)
            Annotation Method                 :: Best-placed reference protein
                                                 set; GeneMarkS-2+
            Annotation Software revision      :: 6.5
            Features Annotated                :: Gene; CDS; rRNA; tRNA; ncRNA
            Genes (total)                     :: 2,776
            CDSs (total)                      :: 2,750
            Genes (coding)                    :: 2,714
            CDSs (with protein)               :: 2,714
            Genes (RNA)                       :: 26
            tRNAs                             :: 24
            ncRNAs                            :: 2
            Pseudo Genes (total)              :: 36
            CDSs (without protein)            :: 36
            Pseudo Genes (ambiguous residues) :: 0 of 36
            Pseudo Genes (frameshifted)       :: 2 of 36
            Pseudo Genes (incomplete)         :: 32 of 36
            Pseudo Genes (internal stop)      :: 2 of 36
            ##Genome-Annotation-Data-END##
FEATURES             Location/Qualifiers
     source          1..6450
                     /organism="Micropruina sp."
                     /mol_type="genomic DNA"
                     /submitter_seqid="NODE_5689_length_6450_cov_4.006880"
                     /isolate="AMR_MDS_2195"
                     /isolation_source="Activated sludge of municipal
                     wastewater treatment plants"
                     /db_xref="taxon:2737536"
                     /environmental_sample
                     /geo_loc_name="Canada"
                     /lat_lon="49.25 N 123.1 W"
                     /collection_date="2015-06-16"
                     /metagenome_source="wastewater metagenome"
                     /note="metagenomic"
     gene            complement(<1..2787)
                     /locus_tag="PLF56_05295"
     CDS             complement(<1..2787)
                     /locus_tag="PLF56_05295"
                     /EC_number="3.1.11.5"
                     /inference="COORDINATES: protein motif:HMM:NF016169.2"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="exodeoxyribonuclease V subunit gamma"
                     /protein_id="HOQ53011.1"
                     /translation="MTGLFLHTAADATALAAELAARLGGTRDDPFVLDVVVTPHAHVR
                     RWLTNELAQRLGRPGEGVCAGISFMSTGRLLRHLGDQGQFWHPRQLAWRLLQAIGTAG
                     DEPQLEQLRRHLAGSRSAYPVALRIATLFQRYLLWRPALVRRWEAGENCDETGTELGF
                     DAWQPVLWRLAAETASPLRVTSEFLAGLRHASAALPLPPTLSLVQPEPLSPWWFDVLT
                     ALAEHREVHVSLREPPAVEPNAAASGPAARLTGFARAANRQLYERAIVRPLPPQPAPS
                     GTLGWLQRTLQGEVLTPPRSDGSVQVHGGHGLERQVEILRDAITALLAADPSLEPRHI
                     VVGCPNLAAAAPLVQAAFRLPAGVPGRHVANDFRVQLADRSSAEVNPLVSVLVQVLTL
                     IDSRAGAAEVLDLCAKPAVAERFNLDADSIERLTRLADEAGVRWGLSSAHRERFGLAR
                     VPQNTWAAGLQRMLLGVALGEDQLPMVGTVLPLQGVGDGDLVALGGLSELISRLSRLA
                     TEAAESTTLAGWVGRLQGALDAFTQVDGDQAWQRTDALLRLADLAERGAGDELLELDD
                     LTALVRDEFERGEARSTFGNGSLTVCSLRSLRGVPYRVVCLLGLDDGVFPRPPERDGD
                     NLMLRAPRPGEPDPPAEDRQALADAVQSARQGVVIVHQARSAQTNEQIPPPAALADLL
                     ELLGRAGVTQRQHPLQPFSPSLFGAGAPPASYDPAGLRGAIALSGPRRPVTPDTPFAP
                     APELTELALDDLLALIKNPVHHFLRERCGLSYWSDEPALDEIPVELNGLQRWAVGERL
                     LTLARTGHPLDRALRAEWLRGVVPPGEIGTRLLNGLARQVTPILDSLPVRPDDPVVHH
                     DIALDLGAVRLTGRVATQQDLVLQVGFGKVSPARRLAVWLHLLALSASTSRPWRGLLV
                     GRGDRVALRAPDRAT"
     gene            2928..4985
                     /locus_tag="PLF56_05300"
     CDS             2928..4985
                     /locus_tag="PLF56_05300"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_002546904.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="ATP-dependent DNA helicase UvrD2"
                     /protein_id="HOQ53012.1"
                     /translation="MPETDELLAALDPEQRAVATTFGVPVCVIAGAGTGKTRAITHRI
                     AHGCATGAYDPFAVLAVTFTTRAAGELRGRLRGLGHPRVQARTFHSAALRQAQYFWPG
                     AMGAELPRVSDQRFGLMAEAAGRQLSQRVETGTLRDLLTEISWAKVSNVPPADYAEIA
                     LAEGREVAAVDATGVGRIYARYEKLKIDRGVIDFDDVLLCAVALLSDHDDVRTQVQRT
                     YRHLVVDEYQDVSPLQQKLLELWLGEHGDLCVVGDPAQTIHRFAGARSTYLTRFAVRH
                     PNAEVIRLVRDYRSTDEVVEVANGVLHSRGPAAGVRLVAQGGSGPAPVFTAAESEADE
                     AARVATWLKQLIADGVPASDLAVLYRIHAQSPAYEAALSQAKVPFTLRSSEGFFQRAE
                     VRGAIVALKAQVAAASDDLLGTVRGVLAGVGWTPEPPTGQGQARERWESLAALLNLAS
                     GHLERHPGAGLPHLLDELDERSRAEHVPSGAGVTLSTLHAAKGLEWTGVALVGLQEGT
                     LPFALATSPAQLAEEQRLLYVGVTRARRHLWLSWAPSRTGGGARRKPSRFLDGIRPET
                     EPVRPARPSGKKKQKAKGLDCRECGRPLAPGAELKLGRHTDCPSSYDENTWALLREWR
                     RQEAAAAGLPAFCIFTDATLMAIAEARPGDERQLLSIAGVGRSKADKYAEAVFAILGQ
                     NSA"
     gene            complement(5005..6342)
                     /locus_tag="PLF56_05305"
     CDS             complement(5005..6342)
                     /locus_tag="PLF56_05305"
                     /EC_number="2.7.-.-"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_007077138.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_function: GO:0005524 - ATP binding [Evidence IEA];
                     GO_function: GO:0016301 - kinase activity [Evidence IEA];
                     GO_process: GO:0016310 - phosphorylation [Evidence IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="AarF/ABC1/UbiB kinase family protein"
                     /protein_id="HOQ53013.1"
                     /translation="MTDPATPEDDRGLPTSSFARASRMLQLPLGAAQRQAVGFGRRLL
                     GADAGQVESDLRSAAAEQMFAVLGELKGGAMKFGQALSMFEAFLPEEIAAPYRQHLSK
                     LQDSAPPLPSSRVQAVLRSELGPRWRERFESIDLRPAAAASIGQVHRGVLVGGQPVAI
                     KVQYPGAGRALRSDLRQIERLAGVVAPLAGGVDVVTITRELAERISEEVDYTLEAQHQ
                     AQAAAAFEGDAEFVVPRVHEATARVLISDWLDGRKLTSVVDSPAEVRNRVGLSYVRFL
                     FAGPARAGLLHADPHPGNYLVLPDGRLGVVDFGLVARLPHGLPPAMGHLIRLAITNDA
                     AAMTAGLAAEGFIAKNVDAGELFGYLEPFVEPARVPEFHFDREWMREQFLHVTSSSTR
                     DGVGMKLTIPPLYALIHRVWLGGIAVLAQLDVTAAFRDVLAEFLPGWNDPQPG"
BASE COUNT          995 a         2326 c         2274 g          855 t
ORIGIN      
        1 ggtggcccga tcgggtgcgc gcagggccac ccggtcgccg cgccccacca gcaggcctcg
       61 ccagggccgt gaggtgctgg ccgacagtgc cagcaggtgc aaccagactg cgaggcggcg
      121 ggccggcgat accttgccga agccgacctg cagcaccaga tcctgttggg tggccacccg
      181 cccggtgagc cgtaccgcgc ccaggtcgag ggcgatgtcg tggtgcacca ccgggtcgtc
      241 tggccgaacc ggcaggctgt cgagaatagg cgtcacctgg cgagccagcc cgttcagcag
      301 ccgcgtgccg atctcgccgg gcgggacgac gccgcgcaac cattccgcgc gcagggcccg
      361 gtcgagcggg tgaccggtgc gggccaacgt cagcaatcgc tcgccgaccg cccagcgctg
      421 cagcccgttc aactcgacgg ggatctcatc gagcgccggc tcgtcgctcc agtaactgag
      481 cccgcaccgt tcgcgcagaa agtggtgcac ggggttcttg atgagcgcga gcaggtcgtc
      541 gagggccagt tcggtcagtt cgggggcggg tgcgaacggg gtatcgggcg tcaccggacg
      601 acgcggaccg ctgagcgcga tcgcgccgcg caaccccgcg gggtcatagc tggccggggg
      661 agcgccggca ccgaacaggg aggggctgaa cggttgcagc ggatgctgtc gctgcgtcac
      721 accggcccgg ccgagcaact cgagcaggtc ggccagcgcg gcgggcggag gaatttgctc
      781 gttggtttgc gccgaacggg cctgatgaac gatcaccacg ccctgccggg cggactggac
      841 ggcatcggcc agcgcctgcc gatcctcggc cggagggtcg ggctcgccgg gacggggtgc
      901 ccgcagcatc aggttgtcgc cgtcgcgttc gggtggtcgc gggaacacgc cgtcgtcgag
      961 acccagcaaa cagacgaccc ggtacggcac gccccgcagc gaccgcagcg agcacaccgt
     1021 cagcgacccg ttgccgaagg tggagcgggc ctcgccgcgc tcgaactcgt cgcgcaccag
     1081 ggcggtgagg tcatccagtt cgagcagctc gtcacccgcg ccacgctcgg ccaggtcggc
     1141 caggcgcagc agcgcgtcgg tgcgctgcca ggcctggtcg ccatcgacct gggtgaaggc
     1201 gtccagggca ccctgtagtc ggcccaccca gcccgcaagg gtggtggact cggccgcctc
     1261 ggtggccagc cgcgaaagcc tgctgatcag ttcggacagg ccacccaggg ccaccaggtc
     1321 gccgtcaccg acaccctgca gcggcagcac ggtgccgacc atcggcaact gatcctcgcc
     1381 gagtgccacg ccgagcagca tgcgttgcag acccgccgcc caggtgttct gcggcacgcg
     1441 cgcaaggccg aaccgctcac gatgcgcgga cgagaggccc cagcgcaccc cggcctcgtc
     1501 ggccagccgg gtgagtcgtt cgatggagtc ggcgtcgagg ttgaagcgct cggccacggc
     1561 cggcttggcg cacaggtcga gcacctcggc ggcgccggcc cgcgagtcga tcaacgtgag
     1621 cacctgcacc aacacgctga ccagtgggtt gacctcagcc gagctgcggt cggccaactg
     1681 cacccggaag tcgttggcga cgtgccgccc cggcaccccc gccggcagcc ggaacgcggc
     1741 ctggacgagc ggcgccgcgg cagccagatt cgggcaaccc accacgatgt gacgcggttc
     1801 gaggctcgga tcagcggcca gcagcgcggt gatcgcatcg cgcaggatct cgacctgacg
     1861 ctcgagaccg tggccgccgt gcacctgcac cgacccatcg cttcggggcg gcgtgagcac
     1921 ctcgccctgc aacgttcgct gcagccagcc cagcgtgccg gacggagccg gctgcggcgg
     1981 caacggacgc acgatcgccc gctcgtacag ctgccggttc gctgcgcgcg cgaagccggt
     2041 gaggcgtgcg gcgggaccgc tcgccgccgc attcggttca acggcggggg gctcgcgcag
     2101 cgacacgtgc acctcgcgat gctcggcgag cgcggtcagc acgtcgaacc accagggtga
     2161 cagcggctcg ggctgcacga gcgacaacgt cggcggtaat ggcaacgccg ccgatgcatg
     2221 gcgcagcccg gccaggaact cgctcgtaac ccgcaggggg ctggccgtct cggcggcgag
     2281 ccgccacagc accggctgcc acgcgtcgaa gccgagttcg gtaccggtct cgtcacagtt
     2341 ctcgccggcc tcccagcgcc gcaccagcgc gggtcgccac agcagatacc gctgaaacaa
     2401 cgtggcgatg cgaagcgcca ccgggtaggc gctgcgcgag ccggccagat ggcgacgcag
     2461 ctgctcgagt tgcggctcgt cgcccgccgt cccgatcgct tgcagcaacc gccacgccaa
     2521 ctgtcgcgga tgccagaact ggccctggtc gccgaggtgc cgtagcaggc ggccggtgct
     2581 catgaagctg atgccggcgc acaccccttc gcccggacgg cccaggcgtt gcgccagctc
     2641 gttggtcaac cagcgacgca cgtgcgcgtg cggggtgacc accacgtcga gcacgaacgg
     2701 atcatcacgc gtacccccca gccgcgccgc cagttcggcg gcgagcgcag tggcgtcggc
     2761 agcggtatgc agaaacagtc cggtcatcgt gggcaccact gtaaaggcgg cgactgacag
     2821 ttctgcgcga accatgcccg ctcgcgcgac cggcggatcc agggctcgcc cagcggcctg
     2881 gtcgccgtta cagattgctg tgggtgcggg cctctacagt gtgcagggtg ccggagaccg
     2941 atgagctgct agccgctctc gaccccgaac agcgggccgt cgcgaccacg ttcggcgtac
     3001 ccgtgtgcgt aatagccggc gcaggcaccg gcaagacccg cgccatcacc caccgcatcg
     3061 cccacggctg cgccaccggc gcctacgacc cgttcgcggt gctggccgtc acgttcacca
     3121 cacgggcggc cggcgagctg cgtgggcggc tgcgcggcct cggccacccg cgggtgcagg
     3181 cgcgcacgtt ccattccgcc gccctgcgtc aggcccagta cttctggccc ggggcgatgg
     3241 gcgccgaact gccgcgggtc agcgatcagc gcttcgggct gatggccgag gcggccggac
     3301 gacagttgag ccaacgggtc gaaaccggca ccctgcgcga cctgctgacc gagatcagct
     3361 gggccaaggt cagtaacgtg ccgccggccg actatgccga gatcgcgttg gccgagggtc
     3421 gcgaggtggc cgccgtcgat gccacggggg tgggccgcat ctacgcccgc tacgaaaagc
     3481 tcaagatcga tcgcggggtg atcgacttcg acgacgtgct gctgtgcgca gtggccctgc
     3541 tcagcgacca cgacgacgtc cgcacccagg tgcagcgcac gtaccggcat ctggtggtcg
     3601 atgagtacca ggacgtcagc ccgctgcagc agaagctgct cgagctgtgg ctgggcgagc
     3661 acggcgacct gtgcgtggtg ggcgacccgg cccagaccat tcaccgcttc gccggcgccc
     3721 ggtcgaccta cctgacccgg ttcgccgtcc ggcatccgaa cgccgaggtg atccggctgg
     3781 tgcgcgacta ccgctccacc gacgaagtgg tcgaggtggc caatggggtg ctgcattccc
     3841 ggggtcccgc cgcaggcgtg cgcctggtcg cccagggagg ctccgggccg gcgccggtct
     3901 tcaccgctgc cgaatccgag gccgacgagg cggcacgggt ggcgacgtgg ctgaagcaac
     3961 tgatcgccga cggggtgccc gcctccgacc tcgccgtgct gtaccgcatt cacgcccagt
     4021 cgccggccta cgaggcggca ctgtcgcagg ccaaggtgcc cttcaccctg cgcagttcgg
     4081 agggcttctt ccagcgcgcc gaggtgcggg gcgcgatcgt ggcactcaag gcgcaggtgg
     4141 cggcggcctc cgacgatctg ctcggcaccg tgcgcggcgt gctggccggg gtcggttgga
     4201 cgcccgagcc gcccaccggt caggggcagg cgcgcgagcg ttgggagtcg ctggccgcac
     4261 tgctcaacct ggcgtccggc cacctggagc ggcaccccgg ggccggcctg ccccaccttc
     4321 tcgacgaact ggacgagcgc tccagggccg agcacgtccc gtccggtgcg ggggtgaccc
     4381 tctcgacgct gcatgcggcc aagggtctcg aatggacggg cgtcgccctg gtcgggctgc
     4441 aggagggcac gttgccgttc gccctggcca cctcgcccgc ccagctcgcc gaagagcagc
     4501 gactgctcta cgtgggcgtc acccgcgccc ggcggcacct gtggctgtcg tgggcgccct
     4561 cacgcaccgg cggcggagcc cgccgcaagc cgtcgcggtt cctggacggt atccgtcccg
     4621 aaaccgaacc ggtgcgaccg gcgcgtccga gcggcaagaa gaagcagaag gcgaaagggc
     4681 tggactgccg cgagtgcggg cgtccgctgg cgccgggggc cgaactgaag ctcggccggc
     4741 acaccgactg cccctcgagc tacgacgaga acacctgggc actactgcgc gagtggcgac
     4801 gccaagaggc cgcggccgcc gggctgccgg cgttctgcat cttcaccgac gccaccttga
     4861 tggcgatcgc cgaagcacga ccgggcgatg agcgccagtt gctgagcatc gccggcgtgg
     4921 gtcgcagcaa ggccgacaag tacgccgagg ccgtcttcgc gatcctcggc cagaactcgg
     4981 cctgaccgcc cgctcagcga ccgatcagcc cggctgcggg tcgttccagc cgggtagaaa
     5041 ctcggccagc acgtcgcgga acgctgcggt cacgtccaac tgggccagca ccgcgatgcc
     5101 gcccagccag acccggtgaa tcagggcata caggggcgga atggtcagct tcatacccac
     5161 cccgtcgcgg gtgctgctgc tggtcacgtg cagaaactgt tcgcgcatcc actcgcggtc
     5221 aaagtgaaac tcgggtactc gagccggttc cacgaacggc tccaggtacc cgaacagttc
     5281 gccggcgtcc acattcttgg cgatgaagcc ttcggcggcc agcccggcgg tcatggccgc
     5341 ggcatcgttg gtgatcgcca acctgatcag gtggcccatc gcgggtggca gcccgtgcgg
     5401 cagccgcgcg accagtccga agtccaccac gccgagacgt ccgtcgggca gcaccaggta
     5461 gttgcccggg tgcggatcgg cgtgcagcag gcccgcccgg gccggcccgg cgaacagaaa
     5521 gcgcacatag gacaacccga cccggttgcg cacctcggcc ggcgaatcca ccaccgaggt
     5581 cagcttgcgc ccgtccagcc agtcgctgat cagcacccgg gcggtggcct cgtgcaccct
     5641 cggcacgacg aactcggcat cgccctcgaa cgcggcggcg gcctgggcct gatgctgcgc
     5701 ctcgagggtg tagtcgacct cttcggagat gcgttcggcc agttcgcggg tgatcgtcac
     5761 cacgtcgacc ccgccggcga gcggggcgac cacaccggcc aaccgctcga tctggcgcag
     5821 gtcggaacgc agcgcccggc cggcccccgg gtactgcacc ttgatcgcca ccggctgccc
     5881 acccaccagc accccacggt gcacctggcc gatggacgca gccgcggcgg gccgcaggtc
     5941 gatcgattcg aaacgctccc gccagcgtgg gcctagctcg ctgcgcagca cggcctgcac
     6001 ccgcgaggac ggcaacgggg gagcgctgtc ctgcagcttg gacaggtgct ggcggtacgg
     6061 agccgcgatc tcttcgggca gaaaggcctc gaacatgctc agggcctggc cgaacttcat
     6121 cgcgccgccc ttgagttcgc cgagcacggc gaacatctgc tcggcggccg cgctgcgcag
     6181 gtcgctttcg acctgcccgg catcggcgcc cagcaaccgg cgtccgaacc cgaccgcctg
     6241 ccgctgcgcc gcgcccagcg gcaactgcag catgcgggac gcccgggcga acgacgaggt
     6301 gggtagcccg cggtcgtcct cgggtgtggc tgggtcggtc atggcgccca ttgtgccaac
     6361 ccactccatg ccaccccgcg ggcaccaggc gcgcctgcac gtgaaacgag cgccacaccg
     6421 accggtgtgg cgctcgctgc gtgggttcag
//
LOCUS       DAOSUG010000118         6387 bp    DNA     linear   ENV 10-JUN-2024
DEFINITION  MAG TPA_asm: Micropruina sp. isolate AMR_MDS_2195
            NODE_5745_length_6387_cov_4.007581, whole genome shotgun sequence.
ACCESSION   DAOSUG010000118 DAOSUG010000000
VERSION     DAOSUG010000118.1
DBLINK      BioProject: PRJNA925477
            BioSample: SAMN32864262
            Sequence Read Archive: SRR4236673
KEYWORDS    WGS; ENV; Metagenome Assembled Genome; MAG; Third Party Data; TPA;
            TPA:assembly.
SOURCE      Micropruina sp. (wastewater metagenome)
  ORGANISM  Micropruina sp.
            Bacteria; Bacillati; Actinomycetota; Actinomycetes;
            Propionibacteriales; Nocardioidaceae; Micropruina.
REFERENCE   1  (bases 1 to 6387)
  AUTHORS   Abdulkadir,N., Saraiva,J.P., Zhang,J., Stolte,S., Gillor,O.,
            Harms,H. and Rocha,U.
  TITLE     Genome-centric analyses of 165 metagenomes show that mobile genetic
            elements are crucial for the transmission of antimicrobial
            resistance genes to pathogens in activated sludge and wastewater
  JOURNAL   Microbiol Spectr 12 (3), e0291823 (2024)
   PUBMED   38289113
REFERENCE   2  (bases 1 to 6387)
  AUTHORS   Abdulkadir,N.
  TITLE     Direct Submission
  JOURNAL   Submitted (24-MAR-2023) Environmental Microbiology, Helmholtz
            Center for Environmental Research -UFZ, Permoserstr 15, Leipzig
            04318, Germany
COMMENT     The annotation was added by the NCBI Prokaryotic Genome Annotation
            Pipeline (PGAP). Information about PGAP can be found here:
            https://www.ncbi.nlm.nih.gov/genome/annotation_prok/
            
            ##Genome-Assembly-Data-START##
            Assembly Method        :: SPAdes v. 3.11.1
            Genome Representation  :: Full
            Expected Final Version :: Yes
            Genome Coverage        :: Not Applicable
            Sequencing Technology  :: Illumina MiSeq
            ##Genome-Assembly-Data-END##
            
            ##Genome-Annotation-Data-START##
            Annotation Provider               :: NCBI
            Annotation Date                   :: 05/04/2023 04:57:10
            Annotation Pipeline               :: NCBI Prokaryotic Genome
                                                 Annotation Pipeline (PGAP)
            Annotation Method                 :: Best-placed reference protein
                                                 set; GeneMarkS-2+
            Annotation Software revision      :: 6.5
            Features Annotated                :: Gene; CDS; rRNA; tRNA; ncRNA
            Genes (total)                     :: 2,776
            CDSs (total)                      :: 2,750
            Genes (coding)                    :: 2,714
            CDSs (with protein)               :: 2,714
            Genes (RNA)                       :: 26
            tRNAs                             :: 24
            ncRNAs                            :: 2
            Pseudo Genes (total)              :: 36
            CDSs (without protein)            :: 36
            Pseudo Genes (ambiguous residues) :: 0 of 36
            Pseudo Genes (frameshifted)       :: 2 of 36
            Pseudo Genes (incomplete)         :: 32 of 36
            Pseudo Genes (internal stop)      :: 2 of 36
            ##Genome-Annotation-Data-END##
FEATURES             Location/Qualifiers
     source          1..6387
                     /organism="Micropruina sp."
                     /mol_type="genomic DNA"
                     /submitter_seqid="NODE_5745_length_6387_cov_4.007581"
                     /isolate="AMR_MDS_2195"
                     /isolation_source="Activated sludge of municipal
                     wastewater treatment plants"
                     /db_xref="taxon:2737536"
                     /environmental_sample
                     /geo_loc_name="Canada"
                     /lat_lon="49.25 N 123.1 W"
                     /collection_date="2015-06-16"
                     /metagenome_source="wastewater metagenome"
                     /note="metagenomic"
     gene            complement(<1..603)
                     /locus_tag="PLF56_05310"
     CDS             complement(<1..603)
                     /locus_tag="PLF56_05310"
                     /EC_number="2.4.-.-"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_013161415.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_function: GO:0016758 - hexosyltransferase activity
                     [Evidence IEA];
                     GO_process: GO:0005975 - carbohydrate metabolic process
                     [Evidence IEA];
                     GO_process: GO:0030259 - lipid glycosylation [Evidence
                     IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="glycosyltransferase"
                     /protein_id="HOQ53014.1"
                     /translation="MVSVVLAGGGTAGHTSPLIATAERIAERDPQGTVVAVGTARGLE
                     TEVVPAAGLRLLLIPPVPLPRSLSPELFAVPWRLVASVRQARRLLKAEGADVVVGFGG
                     YVSLPVYLAAKSLGVPVVIHEQNLLPGLANRVAARFASVVAVSFPDTPLARGRYLGLP
                     VRRGIAELDVAARRPSARQSFGLDPERPTLLVSGGSQGAQS"
     gene            complement(611..1876)
                     /gene="ftsW"
                     /locus_tag="PLF56_05315"
     CDS             complement(611..1876)
                     /gene="ftsW"
                     /locus_tag="PLF56_05315"
                     /inference="COORDINATES: protein motif:HMM:TIGR02614.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_component: GO:0016020 - membrane [Evidence IEA];
                     GO_function: GO:0003674 - molecular_function [Evidence
                     IEA];
                     GO_process: GO:0009252 - peptidoglycan biosynthetic
                     process [Evidence IEA];
                     GO_process: GO:0051301 - cell division [Evidence IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="putative lipid II flippase FtsW"
                     /protein_id="HOQ53015.1"
                     /translation="MAILDTPLEQKKKATKAAAPRARTSLIADALAHPRASYYLVLVP
                     TVLLLALGMMMVLSASSVLGSVRFDDPYYFVKRQVFFLMVGGLAAFVLSKSNTRTLRI
                     LGWVLVVVSLGLQILTFTPLGWGEAKGNRNWVQFGTSFFRIQPSELAKLAIIVWGADL
                     LARKQKLLDQPKHLLIPFLPVSALLIGLVVLNRDLGTGMVMGAIVMAMLWHVGASWRM
                     LGSIAAGVVAVVVALIATSINRMDRILGFFNAEHDVMGVNLQPLRGVFALASGGWWGL
                     GLGASRQKWGSLAEAHTDYVLAIVGEELGLIGTLVVLGLFLVLGYAGFRIALRSDILF
                     CRYMSAAITAWLMIQALVNIMVVLRMIPVFGVTLPLLSYGGSGLMANLAAIGMLLACA
                     KLEPQARKLAERKSRKGAPAVTVVESHRA"
     gene            complement(1882..3372)
                     /gene="murD"
                     /locus_tag="PLF56_05320"
     CDS             complement(1882..3372)
                     /gene="murD"
                     /locus_tag="PLF56_05320"
                     /EC_number="6.3.2.9"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_016669711.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_function: GO:0008764 -
                     UDP-N-acetylmuramoylalanine-D-glutamate ligase activity
                     [Evidence IEA];
                     GO_process: GO:0009252 - peptidoglycan biosynthetic
                     process [Evidence IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="UDP-N-acetylmuramoyl-L-alanine--D-glutamate
                     ligase"
                     /protein_id="HOQ53016.1"
                     /translation="MIDWLPDADRLSPWSQATVVVAGLAASGYAAADGLLELGARVIA
                     VDESDAPAVRERAAILTTLDAEVRIGRGATAELPAGVDLVVTSPGWPPRAPLLAQARQ
                     RGVPIWAEVELAWRMMHPDRVVPWLAVTGTNGKTTTTDMLGSMLAAAGLSVAKVGNIG
                     RPIVEAILDEVDYDVFAVELSAFQLHHCYSLALHSAAVMNLQPDHLEWYGDPAEWGGS
                     AADGMTAYGADKARIYHHVTHACVYNVADQATLRMVEEAEVTEGARAVGFTLGTPAVS
                     MLGVVDDLLVDRAFVEQRRDSALELAKLSDVHPYAPHNVENALAAAALARSFGVPATA
                     VRDGLRNLVLGDHRIQTVAEHDGIRFVDDSKATNPHAAAASMRAFDSIVWIAGGQAKG
                     TTFDDLVQTYADKLRAVVLLGIDRQVIADALARHAPQIPVTVIDSTDTGAMAQAVTAA
                     RSFATSGDVVLLAPGCASLDMFSGYDARGLAFSRAVEAITGADQEG"
     gene            complement(3369..4439)
                     /gene="mraY"
                     /locus_tag="PLF56_05325"
     CDS             complement(3369..4439)
                     /gene="mraY"
                     /locus_tag="PLF56_05325"
                     /EC_number="2.7.8.13"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_016667320.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_function: GO:0008963 -
                     phospho-N-acetylmuramoyl-pentapeptide-transferase activity
                     [Evidence IEA];
                     GO_process: GO:0009252 - peptidoglycan biosynthetic
                     process [Evidence IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="phospho-N-acetylmuramoyl-pentapeptide-
                     transferase"
                     /protein_id="HOQ53017.1"
                     /translation="MRSILLGGGLALLFTLLGTPIAIRVLVKNGYGQFIRDDGPTSHH
                     TKRGTPTMGGIVIIGSVILAYALAHLLTWSPPTASGLLVLYLVTGLGAVGFADDWTKI
                     SRARSLGLSGRVKLICQGAVGVSFAILSFQFPDDQGITPASNAISFLRDISWLQLPLW
                     LAVIWVVLIIASWSNAANLADGLDGLATGAAAMVFAAYAVVNIWQNNQSCSTLSTAGP
                     QCYWVRDPRDLALVSLALAGSCFGFLWWNASPAKIFMGDTGSLAIGGGFAALSIFTRT
                     ELLMVIIAGLFVVITMSVVLQVGWFKLTKGKRLFKMAPLHHHFELLGWAQVTVVIRFW
                     IFCGLCVAIGLGIFYGEWTVGQ"
     gene            complement(4436..5845)
                     /gene="murF"
                     /locus_tag="PLF56_05330"
     CDS             complement(4436..5845)
                     /gene="murF"
                     /locus_tag="PLF56_05330"
                     /EC_number="6.3.2.10"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_018384164.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_function: GO:0005524 - ATP binding [Evidence IEA];
                     GO_function: GO:0047480 -
                     UDP-N-acetylmuramoyl-tripeptide-D-alanyl-D-alanine ligase
                     activity [Evidence IEA];
                     GO_process: GO:0009252 - peptidoglycan biosynthetic
                     process [Evidence IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="UDP-N-acetylmuramoyl-tripeptide--D-alanyl-D-
                     alanine ligase"
                     /protein_id="HOQ53018.1"
                     /translation="MRERSIGQVAALAGAAAVSVDGTAGPAVVIDSRLASPGAVFVAL
                     PGERVDGHDFIGAAAAGGAVAALCTRVTDAPLAHLVVADALQGLTDLARGVVAEAKVE
                     GLRCVAVTGSSGKTSTKDLLGQVLAAHAPTVSPQGSFNNEIGVPLTALRVNADTRYLV
                     SEMGARGGGHIAWLCGIVPPDVGLVLNVGHAHLGEFGSVAGIAQAKGEVVEALSPQGW
                     AVLNADDALVSAMAARTSAHLAYFSMTGEPAAGELRVWAENLEADDLQRHSFRLRSAG
                     TVHADQPVGLQGSGRHQVANALAAAAVGLALGLPAAGVAAALSAATAMSGWRMQLLPR
                     ADGVLVINDAYNANPDSMHAALTTVAAMRRPGGRLVAVLGDMLELGEGAPVEHENVGR
                     LAAELGFDVVAIGALAAEIARGVQRASGTVTVVNDPQEAVAVTERDLQPSDLVVVKAS
                     RGLALERVADALYAGGERA"
     gene            complement(5838..>6387)
                     /locus_tag="PLF56_05335"
     CDS             complement(5838..>6387)
                     /locus_tag="PLF56_05335"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_013161420.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_function: GO:0005524 - ATP binding [Evidence IEA];
                     GO_function: GO:0016874 - ligase activity [Evidence IEA];
                     GO_process: GO:0009058 - biosynthetic process [Evidence
                     IEA]"
                     /codon_start=2
                     /transl_table=11
                     /product="cyanophycin synthetase"
                     /protein_id="HOQ53019.1"
                     /translation="VQAAGLDAQRAATGLAQAFVPGRMQRVPLGAAAPRVVVDFAHTP
                     EAVAAALAGLPAPRIVVLGAGGDRDPGKRGPMGAAAAANAEVVIVTDDNPRSEAPESI
                     RAQVLRGARTAGGAEVIDGGDRRSAIALALARATPDAWVAVLGKGHESGQEIAGVVRP
                     FDDVAVVRDAWQGVRGGSADGA"
BASE COUNT         1063 a         2313 c         2180 g          831 t
ORIGIN      
        1 gctctgggcg ccctgggaac cgccggagac cagcaacgtc ggacgctcgg ggtcgagccc
       61 gaacgactgc cgcgcgctcg gacggcgcgc cgcgacgtcg agttcagcga tgccgcggcg
      121 caccggcaga cccaggtagc ggccccgcgc gagcggcgtg tcgggaaacg agaccgccac
      181 cacggacgcg aaccgcgcgg ccacccggtt ggccagcccc ggcagcaggt tctgctcatg
      241 gatcaccacc ggcacgccca ggctcttggc ggccaggtac accggcagcg acacgtaccc
      301 accgaagccg acgaccacgt cggcgccttc ggccttgagc agccgtctgg cctgccgcac
      361 cgaggccacc aagcgccagg gaacggcgaa cagttcgggg ctgagcgagc gcggcagtgg
      421 caccggtggg atcagcagca gccgcagccc ggcagccggc accacctcgg tctcgaggcc
      481 gcgggcggtt ccgaccgcga ccaccgtgcc ttgcgggtcg cgctcggcga tgcgctcggc
      541 ggtggcgatc aacggcgagg tgtggccggc ggtgcctccc ccggccagca cgacggaaac
      601 catggctctc ctaggcgcgg tgcgactcga ccacggtgac cgcgggagcg cccttgcgcg
      661 acttgcgttc ggccagctta cgggcctgtg gctcgagctt ggcgcaggcc agcagcatgc
      721 cgatggccgc caggttggcc atcagacccg accccccgta cgacaacagg ggcagcgtga
      781 caccgaacac cggaatcatg cgcagcacca ccatgatgtt caccagggcc tgaatcatca
      841 gccaggcggt gatcgccgcc gacatgtagc ggcagaacag aatgtcggag cgcagcgcga
      901 tgcgaaaacc ggcgtagccc agcaccagga acagccccag caccaccagg gtgccgatca
      961 ggcccagttc ttcaccgacg atggccagca cgtagtcggt gtgggcctcg gccagcgagc
     1021 cccacttctg cctggacgcc cccagaccca gcccccacca gccgcccgaa gccagcgcga
     1081 acacaccgcg cagcggctgc aggttgacgc ccatcacgtc gtgctcggcg ttgaagaagc
     1141 cgaggattcg atccatacgg ttgatcgagg tggcgatcag cgccaccacg acggcgacca
     1201 caccggcggc gatcgagccc agcatgcgcc aggacgcccc gacgtgccac agcatggcca
     1261 tcacgatggc gcccatcacc atgccggtgc cgaggtcgcg gttgagcacc accaggccga
     1321 tcagcagggc agagaccggc aggaacggga tcagcaggtg cttgggctgg tcgagcagct
     1381 tctgcttgcg ggccagcagg tcggcacccc acacgatgat cgccagcttg gcgagttcgg
     1441 acggctggat gcgaaagaac gaggtgccga actgcaccca gttgcggttg cccttggcct
     1501 cgccccaccc cagtggggtg aaggtcagaa tctgcaggcc gagcgagacc accaccagca
     1561 cccagcccag aatgcggagc gtccgggtgt tgctcttgga cagcacgaag gcggccaggc
     1621 cgccgaccat cagaaagaac acctgccgct tgacgaagta gtacggatcg tcgaaacgga
     1681 cgctgcccag cacgctcgat gccgagagca ccatcatcat gcccagggcc agcagcagca
     1741 ccgttggcac cagcaccagg tagtagctgg cccgcgggtg agcgagtgcg tcggcgatca
     1801 gcgaggtgcg ggcgcggggt gcggcggctt tcgtggcctt cttcttctgc tccagcgggg
     1861 tgtcgagaat cgccactgct gtcagccctc ctggtcggcc ccggtgatcg cctctacagc
     1921 ccgggagaac gccagacctc gcgcgtcgta cccgctgaac atgtccaaac tggcgcagcc
     1981 gggggcgagc aacacgacgt caccggaagt ggcgaacgag cgtgcggccg tgaccgcctg
     2041 agccattgct ccagtgtcgg tggaatcgat caccgtgacg gggatctgcg gcgcgtgtcg
     2101 ggcgagtgca tcggcgatca cctgccgatc gatgccgagc agcaccaccg cccgcagttt
     2161 gtcggcatag gtctgcacca gatcgtcgaa cgtggtgccc ttggcctggc cgccggcgat
     2221 ccacacgatc gagtcgaagg cgcgcatgga cgcggccgcc gcgtgcgggt tggtggcctt
     2281 ggagtcgtcc acgaagcgga tgccgtcgtg ctcggcaacg gtctggatgc ggtgatcgcc
     2341 cagcaccagg ttgcgcagcc cgtcgcgcac ggcggtcgcg ggcaccccga acgaacgggc
     2401 cagcgcggcc gcggccagcg cgttctcgac gttgtgcggc gcgtaggggt gcacgtcgga
     2461 tagtttggcc agttcgaggg ccgagtcgcg gcgttgctcg acgaaggcgc ggtcgaccag
     2521 caggtcgtcc accacgccca gcatcgagac cgcaggcgtg ccgagcgtga agccgaccgc
     2581 ccgggcgcct tcggtgacct cggcctcttc gaccatgcgc agggtggcct ggtcggcgac
     2641 gttgtagacg caggcgtggg tgacgtggtg gtagatccgg gccttgtccg caccgtaggc
     2701 ggtcatgccg tccgcggccg agccgcccca ctcagcgggg tcgccgtacc actccagatg
     2761 atcgggctgc aggttcatca ccgccgccga gtgcagggcc agcgagtagc agtgatgcag
     2821 ctggaaggcg gacagttcga cggcgaacac gtcgtagtcg acctcgtcca gaatggcctc
     2881 gacgatcgga cggccgatgt tgcccacctt ggccacgctc agcccggcgg cggccagcat
     2941 cgagcccagc atgtcggtgg tggtggtctt gccgttggtg ccggtgacgg ccagccaggg
     3001 caccacccgg tcggggtgca tcatgcgcca ggccagttcg acctcggccc agatcggtac
     3061 cccgcgttgg cgcgcctggg ccagcagcgg tgcgcgcggc ggccaacccg gtgaagtcac
     3121 gaccaggtcg acgccggccg gcagctcggc ggtggcgccc ctgccgatgc gcacctcggc
     3181 gtcgagggtg gtcaggatcg cggccctctc gcggacggcg ggagcgtccg actcgtcgac
     3241 cgcgatcacc cgtgccccga gctcgagcag gccgtcggcc gccgcatatc cggacgccgc
     3301 caggccggcg accaccaccg tggcctgcga ccagggtgag agccggtcgg catcaggcaa
     3361 ccagtcgatc attggcccac cgtccattcg ccgtagaaga tgccgagccc gatggcaacg
     3421 cacaggccgc agaagatcca gaaccggatc accacggtga cctgcgccca gccgagcagt
     3481 tcgaagtggt ggtgcagcgg agccatcttg aacagccgct tgcccttggt cagcttgaac
     3541 cagccgacct gcagcaccac cgacatggtg atcaccacga acagcccggc gatgatcacc
     3601 atcagcagtt cggtgcgggt gaagatcgac agcgccgcga aaccgccgcc gatggccagc
     3661 gagccggtgt cgcccatgaa gatcttggcc gggctggcgt tccaccacag gaacccgaag
     3721 caggaaccgg ccagggccag cgagaccagg gccaggtcac gggggtcgcg cacccagtag
     3781 cactgcgggc cggccgtcga cagggtggaa caggactggt tgttctgcca gatgttgacc
     3841 accgcgtagg ccgcgaagac catcgcggcg gcaccggtgg ccagcccgtc caggccgtcg
     3901 gccaggttgg cggcgttcga ccacgaggcg atgatcagca cgacccagat caccgccaac
     3961 cacagcggca gctgtagcca actgatgtcg cgcagaaacg agatggcgtt gctggccggg
     4021 gtgatgccct ggtcgtcggg aaactggaac gacaggatcg cgaacgacac cccgaccgct
     4081 ccctggcaga tcagcttgac ccggccgctc aggccgagcg agcgggcccg cgagatcttg
     4141 gtccagtcgt ccgcgaaacc gaccgcgccg agcccggtga ccaggtacag caccaacagc
     4201 ccggacgccg tcggcggcga ccaggtgagc aggtgggcca gcgcgtaggc caggatcacc
     4261 gaaccgatga tcacgatgcc gcccatggtg ggcgtgccgc gcttggtgtg gtgcgaggtg
     4321 ggcccgtcgt cgcggatgaa ctggccataa ccgttcttga ccagcacccg aatcgcgatc
     4381 ggggtgccca gcagggtgaa taacagcgcc agcccgccgc ccagcagaat gcttctcatg
     4441 cgcgctcgcc cccggcgtac agggcgtcgg ccacccgctc gagcgccagc ccccgcgacg
     4501 ccttcaccac gaccagatcg gacggttgca ggtctcgttc ggtcaccgcc accgcctcct
     4561 gaggatcgtt caccaccgtt actgtgccgg acgcccgctg cacaccgcga gcgatctcgg
     4621 cagccagcgc gccgatcgcc accacgtcga agcccagctc ggcggccagc cgtcccacgt
     4681 tctcatgctc caccggcgca ccctcgccca gttcgagcat gtcgcccaga acggccacca
     4741 gccgtccacc ggggcggcgc atggcggcga ccgtggtcag ggcggcgtgc atggagtcgg
     4801 ggttggcgtt gtaggcgtcg ttgatcacca gcaccccgtc ggcgcgcggc agcagctgca
     4861 tgcgccaccc cgacatcgca gtcgcggcgc tgagcgcggc cgcgacgccg gcggcgggta
     4921 gccccaaagc caggccgacg gcggccgctg ccagcgcgtt ggcgacctga tgccgtccgg
     4981 agccctgcag gccgaccggc tgatcggcat gcaccgtgcc cgcgctgcgc agcctgaacg
     5041 aatgccgctg caggtcgtcg gcctcgaggt tctcggccca gacccgaagc tcgccggcgg
     5101 ccggttcgcc ggtcatcgag aagtacgcga ggtgggcact ggtgcgtgcg gccatggcgc
     5161 tgaccagggc gtcatcggcg ttgagcacgg cccacccctg cgggctgagc gcttcgacca
     5221 cttcgccctt ggcctgcgcg atgcccgcca ccgagccgaa ctcgcccagg tgcgcgtgcc
     5281 cgacgttgag cacgagaccg acatcgggcg gcacgatgcc gcacagccac gcgatgtgac
     5341 caccgccgcg ggcccccatc tcgctgacca ggtagcgcgt gtcggcgttc acccgcagcg
     5401 cggtcagcgg cacgccgatc tcgttgttga aggagccctg cgggctgacc gtcggcgcgt
     5461 gcgcggccaa cacctggccc agcaggtcct tggtgctggt cttgccggac gatccggtca
     5521 cggccacgca acgcaggccc tcgaccttgg cctcggccac cacaccccgg gccaggtcgg
     5581 tcaggccctg cagggcatcg gccaccacca ggtgcgccag tggcgcgtcg gtgacccggg
     5641 tgcacagcgc cgccaccgct cccccggccg cggcggcacc gatgaagtcg tgcccgtcga
     5701 cccgctcgcc gggcagcgcg acgaacaccg cccccggcga ggccagcctc gagtcgatca
     5761 ccaccgcggg cccggcggtg ccgtcgacac tcaccgcggc ggccccggcc agggccgcca
     5821 cctgcccaat gcttcgttca cgcaccgtct gcactccccc cgcggacacc ctgccacgca
     5881 tcgcggacga ccgcgacatc gtcgaacgga cgaaccacgc ccgcgatttc ctggccgctc
     5941 tcgtgcccct tgccgagcac cgccacccag gcgtcggggg tggcccgggc aagggccaac
     6001 gcgatcgccg aacggcgatc accgccgtcg atcacctcgg caccgccggc cgtccgggcc
     6061 ccccgcagca cctgcgcgcg aatcgactcc ggtgcctccg agcggggatt gtcgtcggtg
     6121 acgatcacca cctcggcgtt cgcggcggca gcggccccca tcggcccacg cttgccgggg
     6181 tcgcgatcgc cgccggcgcc cagcaccacg attcggggag ccggcaggcc ggccaacgcg
     6241 gccgccaccg cctcgggcgt gtgggcgaag tcgaccacca cgcggggggc cgcggcgccc
     6301 aacgggacgc gttgcatgcg gcccggcacg aaagcctgcg ccagacccgt cgcagcccgc
     6361 tgggcgtcga gcccggcggc ctgcacc
//
LOCUS       DAOSUG010000119         6385 bp    DNA     linear   ENV 10-JUN-2024
DEFINITION  MAG TPA_asm: Micropruina sp. isolate AMR_MDS_2195
            NODE_5747_length_6385_cov_4.668088, whole genome shotgun sequence.
ACCESSION   DAOSUG010000119 DAOSUG010000000
VERSION     DAOSUG010000119.1
DBLINK      BioProject: PRJNA925477
            BioSample: SAMN32864262
            Sequence Read Archive: SRR4236673
KEYWORDS    WGS; ENV; Metagenome Assembled Genome; MAG; Third Party Data; TPA;
            TPA:assembly.
SOURCE      Micropruina sp. (wastewater metagenome)
  ORGANISM  Micropruina sp.
            Bacteria; Bacillati; Actinomycetota; Actinomycetes;
            Propionibacteriales; Nocardioidaceae; Micropruina.
REFERENCE   1  (bases 1 to 6385)
  AUTHORS   Abdulkadir,N., Saraiva,J.P., Zhang,J., Stolte,S., Gillor,O.,
            Harms,H. and Rocha,U.
  TITLE     Genome-centric analyses of 165 metagenomes show that mobile genetic
            elements are crucial for the transmission of antimicrobial
            resistance genes to pathogens in activated sludge and wastewater
  JOURNAL   Microbiol Spectr 12 (3), e0291823 (2024)
   PUBMED   38289113
REFERENCE   2  (bases 1 to 6385)
  AUTHORS   Abdulkadir,N.
  TITLE     Direct Submission
  JOURNAL   Submitted (24-MAR-2023) Environmental Microbiology, Helmholtz
            Center for Environmental Research -UFZ, Permoserstr 15, Leipzig
            04318, Germany
COMMENT     The annotation was added by the NCBI Prokaryotic Genome Annotation
            Pipeline (PGAP). Information about PGAP can be found here:
            https://www.ncbi.nlm.nih.gov/genome/annotation_prok/
            
            ##Genome-Assembly-Data-START##
            Assembly Method        :: SPAdes v. 3.11.1
            Genome Representation  :: Full
            Expected Final Version :: Yes
            Genome Coverage        :: Not Applicable
            Sequencing Technology  :: Illumina MiSeq
            ##Genome-Assembly-Data-END##
            
            ##Genome-Annotation-Data-START##
            Annotation Provider               :: NCBI
            Annotation Date                   :: 05/04/2023 04:57:10
            Annotation Pipeline               :: NCBI Prokaryotic Genome
                                                 Annotation Pipeline (PGAP)
            Annotation Method                 :: Best-placed reference protein
                                                 set; GeneMarkS-2+
            Annotation Software revision      :: 6.5
            Features Annotated                :: Gene; CDS; rRNA; tRNA; ncRNA
            Genes (total)                     :: 2,776
            CDSs (total)                      :: 2,750
            Genes (coding)                    :: 2,714
            CDSs (with protein)               :: 2,714
            Genes (RNA)                       :: 26
            tRNAs                             :: 24
            ncRNAs                            :: 2
            Pseudo Genes (total)              :: 36
            CDSs (without protein)            :: 36
            Pseudo Genes (ambiguous residues) :: 0 of 36
            Pseudo Genes (frameshifted)       :: 2 of 36
            Pseudo Genes (incomplete)         :: 32 of 36
            Pseudo Genes (internal stop)      :: 2 of 36
            ##Genome-Annotation-Data-END##
FEATURES             Location/Qualifiers
     source          1..6385
                     /organism="Micropruina sp."
                     /mol_type="genomic DNA"
                     /submitter_seqid="NODE_5747_length_6385_cov_4.668088"
                     /isolate="AMR_MDS_2195"
                     /isolation_source="Activated sludge of municipal
                     wastewater treatment plants"
                     /db_xref="taxon:2737536"
                     /environmental_sample
                     /geo_loc_name="Canada"
                     /lat_lon="49.25 N 123.1 W"
                     /collection_date="2015-06-16"
                     /metagenome_source="wastewater metagenome"
                     /note="metagenomic"
     gene            <1..1064
                     /gene="argG"
                     /locus_tag="PLF56_05340"
     CDS             <1..1064
                     /gene="argG"
                     /locus_tag="PLF56_05340"
                     /EC_number="6.3.4.5"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_014847869.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_function: GO:0004055 - argininosuccinate synthase
                     activity [Evidence IEA];
                     GO_process: GO:0042450 - arginine biosynthetic process via
                     ornithine [Evidence IEA]"
                     /codon_start=3
                     /transl_table=11
                     /product="argininosuccinate synthase"
                     /protein_id="HOQ53020.1"
                     /translation="GVDIWGDGSTYKGNDIERFYRYGLLANPRLRIYKPWLDHDFVSE
                     LGGRTEMSQWLAERNLPYRDSKEKAYSTDANIWGATHEAKDLESLDASIEIVEPIMGV
                     RFWDPAVEIATEDVTIAFEQGRPVSINGVTFADAVALVREANAIGGRHGLGMADQIEN
                     RIIEAKSRGIYEAPGMALLHIAYERLLNAIHNEDTLANYYTEGRKLGRLMYEGRWLDP
                     QSLMLRESLQRWVGSAVTGSVTLRLRRGDDYSILSTEGPALSYHPEKLSMERVGDAAF
                     GPVDRIGQLTMRNLDIADSRTRLEQYAHLGLVGGAVSSLVGELEPGEARGITELTATV
                     FPSEIDDATADGAMEFGAD"
     gene            1104..1631
                     /locus_tag="PLF56_05345"
     CDS             1104..1631
                     /locus_tag="PLF56_05345"
                     /inference="COORDINATES: ab initio
                     prediction:GeneMarkS-2+"
                     /note="Derived by automated computational analysis using
                     gene prediction method: GeneMarkS-2+."
                     /codon_start=1
                     /transl_table=11
                     /product="hypothetical protein"
                     /protein_id="HOQ53021.1"
                     /translation="MQSLIDHALDAANDSLAEAFLDSRRVFWVGNSDDPSAIAAECSE
                     AARERVTLADASADAFGTLLALNRTLAGRVEIRLVLYSVGRDALAFVVLPAAEWAQLD
                     DDIGDALAQVVWELNDDLDPFGQLQGTDVIDPYHELLAENPGRYGECLARALADPASA
                     GGEPHSDDADEVMPW"
     gene            1760..2221
                     /locus_tag="PLF56_05350"
     CDS             1760..2221
                     /locus_tag="PLF56_05350"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_002545424.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="Hsp20/alpha crystallin family protein"
                     /protein_id="HOQ53022.1"
                     /translation="MARTFDPFRDLDRWFTDVARTPASAAMAMDLYKAGDNFVARVDL
                     PGVDPGTIDVDIDERTLTIRAERKADATEGDLRWLVRERPVGTFARQLTLGYGVALDR
                     IAAEYNDGVLTLTIPVAEEAKPRKVAVSHTGGATQIEGSVDESGEPEHEQN"
     gene            complement(2295..3005)
                     /locus_tag="PLF56_05355"
     CDS             complement(2295..3005)
                     /locus_tag="PLF56_05355"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_013864268.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_function: GO:0016765 - transferase activity,
                     transferring alkyl or aryl (other than methyl) groups
                     [Evidence IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="isoprenyl transferase"
                     /protein_id="HOQ53023.1"
                     /translation="MPEHVAIVMDGNGRWAKARNLPRTEGHARGEDSLFDVIEGAIEV
                     GLKYLSAYAFSTENWRRSPDEVRWLMGFNRDVIHRRRDQLDDMGVRIRWAGRRPKLWR
                     SVIRELEEAEEQSRDNTTLTLQFCVNYGGRAEIVDAARAIAKAAQAGRLDPDHLTEKT
                     FRRFLDEPQIPDVDLFVRSSGEQRTSNFLLWQSAYAELVFLDRLWPDFDRRDLWRAIE
                     LYADRDRRYGGAVPNQVE"
     gene            3190..4074
                     /locus_tag="PLF56_05360"
     CDS             3190..4074
                     /locus_tag="PLF56_05360"
                     /inference="COORDINATES: protein motif:HMM:NF014072.2"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="LarC family nickel insertion protein"
                     /protein_id="HOQ53024.1"
                     /translation="MHAWLDVTAGVAGDMLMGALVDAGAPLEGIQQAVRAVAGPGVAL
                     RREEVTRGGMRATKVHVDLAVAGTGGRSWPQLRETLLSADIHPATRQTAIRVFERFVA
                     AYGRGHGIAEDEVTLAEVGALDSLSDIVGDCEALRLLGVTSVSASPVAVGAGRIHTSH
                     GDLPVPVPAVAELALGWGISRPAAALDGPDAEGAADWDSPDPDGAVTDTALDVRHLPM
                     ANATVVDGMVTEGPARSVEPGQLGELATPTGMALLRGLAERCEPLPPMTLAALGVGAG
                     GRDVPGRPNVVRVLLGSR"
     gene            4133..4804
                     /locus_tag="PLF56_05365"
     CDS             4133..4804
                     /locus_tag="PLF56_05365"
                     /inference="COORDINATES: protein motif:HMM:NF016300.2"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="SIMPL domain-containing protein"
                     /protein_id="HOQ53025.1"
                     /translation="MEISVQGTHEQTVRPEVARLHARAHAEAGGKDEVLRTATDVVQQ
                     LSGELNRLKEAGAVREVVVRPISTSSWRPVARGRQQPPIYRADASLQADFTDFAALAD
                     LAARFGAVDGLELNWVEWRLTDATRLELEAVCLTRAVEQARERALVMARAAGESSVAF
                     VQLADPGLLTDNPIRREAFEAPPAMGAMMRSAKMADELAGIELQPEDLTVSGTVQARF
                     VTTQG"
     gene            4814..5554
                     /gene="recO"
                     /locus_tag="PLF56_05370"
     CDS             4814..5554
                     /gene="recO"
                     /locus_tag="PLF56_05370"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_002523884.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_function: GO:0003677 - DNA binding [Evidence IEA];
                     GO_process: GO:0006281 - DNA repair [Evidence IEA];
                     GO_process: GO:0006310 - DNA recombination [Evidence IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="DNA repair protein RecO"
                     /protein_id="HOQ53026.1"
                     /translation="MPTYRDEAVVLRTHKLGEADRIITLLSRRNGKIRAVAKGVRRTT
                     SKFGGRLEPFSHIDAQFATGRTLDVVTQAVTLHAYTEPMGNDYGAYTSGQVILETADR
                     LVAEEREPSLQQFLLLVGALNAMTAGTADGPRPATMIMDSYLLRAVAVAGWAPSLSDC
                     ARCGQVGPHEAFNPQAGGMVCRRCRPPASALPSAAVIGYLSALLTGDWPATREVDERA
                     QRTASGLVSAFANWHLERGLRSLIHVDR"
     gene            5584..5886
                     /locus_tag="PLF56_05375"
     CDS             5584..5886
                     /locus_tag="PLF56_05375"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_014846479.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="antibiotic biosynthesis monooxygenase"
                     /protein_id="HOQ53027.1"
                     /translation="MLAVNRFRVADEHAFVPRAAAAVAFLSECDGNLSVDLLRNLDEP
                     DLWCLVSSWRNVGSYRRSFNGYTAKLVLVPLLSEAIDEPGAYDHPEAVGENRPRTR"
     gene            5917..>6385
                     /locus_tag="PLF56_05380"
     CDS             5917..>6385
                     /locus_tag="PLF56_05380"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_002545536.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="glycine--tRNA ligase"
                     /protein_id="HOQ53028.1"
                     /translation="MDSQGVSVASRLDNVISLCKRRGFVYPCGEIYGGTRAAWDYGPY
                     GVELKENIKRQWWKAVVQGRDDVVGIDSSIILPRQVWVASGHVGVFSDPLTECLSCHK
                     RFRADQLIENFEHKKGRAPSGLEEINCPECGTLGRFTEPRDFNMMLKTYLGVIE"
BASE COUNT         1026 a         2169 c         2260 g          930 t
ORIGIN      
        1 acggcgtcga catctggggc gacggctcca cctacaaggg caacgacatc gagcgcttct
       61 accgctacgg cctgctcgcc aacccgcggc tgcgcatcta caagccgtgg ctcgaccacg
      121 acttcgtcag cgaactgggc ggacgcaccg agatgagcca gtggctggcc gaacgcaacc
      181 tgccgtatcg cgattccaaa gagaaggcct actcgaccga cgccaacatc tggggcgcca
      241 cccacgaggc caaagacctc gaatcgctcg atgccagcat cgagatcgtc gaaccgatca
      301 tgggcgtgcg gttctgggat ccggcggtcg agatcgccac cgaagatgtc acgatcgcgt
      361 tcgagcaggg ccgtccggtc agcatcaacg gggtcacgtt cgccgacgcg gtcgccctgg
      421 tgcgcgaggc caacgcgatc ggcggacggc acgggctcgg catggccgac cagatcgaga
      481 accgcatcat cgaggcgaag agccgcggca tctacgaggc cccgggcatg gcgctgctgc
      541 acatcgccta cgagcggctg ctgaacgcga tccacaatga ggacaccctg gccaactact
      601 acaccgaagg ccgcaagctg ggccggctga tgtacgaggg tcgctggctc gacccgcagt
      661 cgttgatgct gcgcgagtcg ctgcaacgct gggtcggctc ggcggtgacc ggctcggtca
      721 cgctgcggtt gcgccgcggc gacgactact cgattctgag caccgagggt ccggcgctgt
      781 cgtaccaccc cgagaagctg tccatggagc gggtcggcga cgccgccttc ggaccggtcg
      841 atcgcatcgg ccagctgacc atgcgaaatc tcgacatcgc cgactcgcgc acccggttgg
      901 agcagtacgc gcacctcggc ctggtcggcg gggcggtctc atccctggtc ggcgaactcg
      961 agcccggcga ggcacgcggc atcaccgagt tgacggccac ggtgtttccc tccgaaatcg
     1021 acgacgcgac cgccgatggc gcgatggagt tcggcgccga ctgaccccgc gtcacgggcc
     1081 gggccgttag gctcatagtc accatgcagt cgctcatcga tcacgccctg gacgccgcca
     1141 acgacagtct ggccgaggcg ttccttgact cgcgccgggt gttctgggtc ggcaacagcg
     1201 acgacccgtc tgccatcgcc gccgagtgca gcgaggcggc gcgggagcgg gtcacgctcg
     1261 ccgacgcttc agccgacgct ttcggcacgc tgctggcgct caacagaaca ctggccggac
     1321 gcgtcgagat tcgcctggtg ttgtactcgg tgggccggga cgcactggcg ttcgtcgtac
     1381 tgccggccgc ggaatgggcg caactcgacg acgacatcgg cgacgcgctg gcgcaggtgg
     1441 tgtgggagct caacgacgac ctcgacccgt tcggacagct gcagggcacc gatgtgatcg
     1501 acccctacca cgagttgctg gccgagaacc ccggccggta cggggagtgt ctcgcccggg
     1561 cactcgccga ccccgcctcg gccggcggcg agccgcactc cgacgatgcc gacgaggtca
     1621 tgccctggtg attcgcgtcc gacaagggtg tcggcacgca ttgagcgcag cacgctcaac
     1681 tctggaatag atcgggcgcg ggctctgttc gaataggtgt atgtcagtgc caaccgaaca
     1741 aaccaaagga gtgatcacca tggcacgtac cttcgatccc ttccgcgacc tggaccgctg
     1801 gttcaccgat gtggcccgca ccccggccag cgccgcgatg gcgatggacc tgtacaaggc
     1861 gggcgacaac ttcgtcgcac gcgtcgacct gcccggagtc gaccccggca ccatcgacgt
     1921 cgacatcgac gagcgcacgc tgaccatccg tgccgagcgc aaggccgacg ccaccgaggg
     1981 cgatctgcgc tggctggtgc gcgagcgtcc ggtcggcacg ttcgcccggc agctcaccct
     2041 gggctacggg gtcgcgctcg atcgcatcgc cgccgagtac aacgacgggg tgctgacgct
     2101 gaccattccg gtggccgaag aggccaagcc gcgcaaggtc gccgtctcgc acaccggagg
     2161 cgccacgcag atcgagggat ccgtggacga atccggcgaa ccggagcacg agcagaactg
     2221 agctcggccc aacggccccg accacagcgg tcggggccgt tgtgcgtctg ccgagcgtgt
     2281 cctccggagg cttctcactc gacctggttg ggcaccgcgc cgccgtagcg acggtcgcga
     2341 tcggcgtaca gctcgatcgc ccgccacagg tcgcgacggt cgaagtcggg ccacagccgg
     2401 tcgagaaaga ccagttcggc atacgccgac tgccacagca gaaaattgga cgtacgctgc
     2461 tcgcccgagg agcgcacgaa caggtccacg tcgggaatct ggggctcgtc cagaaagcgc
     2521 cggaaggtct tctcggtcag gtgatcgggg tcgagccggc cggcctgggc agccttggcg
     2581 atggcgcggg cggcgtccac gatctcggcg cgcccgccgt agttgacgca gaactgcagc
     2641 gtgagcgtgg tgttgtcccg gctctgttcc tcggcctctt cgagttcgcg gatcaccgag
     2701 cgccacagct tgggccggcg tcccgcccag cggatgcgca cgcccatgtc gtccagctgg
     2761 tcacgccggc ggtggatcac gtcgcggttg aagcccatca gccaacgcac ctcgtcgggg
     2821 gaacgccgcc agttctcggt cgagaacgcg taggccgaga ggtacttcag gcccacctcg
     2881 atcgcgccct cgatcacgtc gaacagcgaa tcctcgccgc gcgcgtggcc ctcggtgcgg
     2941 ggcaggttgc gcgccttggc ccagcgtccg ttgccgtcca tcacgatcgc gacgtgctcg
     3001 ggcatcgcct tgcgggggat cgccggcgga cgtgcgccac tggggtgcgg gatcggcgga
     3061 cggggcgtct tcggcaccct tggagcctag tgccgtccgt gcgccggcgg ccaacaggga
     3121 aggatccagc agggaaggat cggcaacacg ggacccggcg gggtcgctga gtgctccatc
     3181 atggcgggta tgcacgcgtg gctggatgtg accgccggag tggccggaga catgctgatg
     3241 ggcgcactgg tggacgccgg ggcgccgctc gagggcatac agcaggcggt gcgggcggtg
     3301 gccggtcccg gggtggcgct gcggcgcgag gaggtcaccc ggggcggcat gcgagccacc
     3361 aaagtgcacg tagacctggc cgtcgcgggc accggcggca ggtcgtggcc ccaactgcgc
     3421 gagacgctgc tgtcggcgga catccatccg gccacccggc agaccgcgat ccgggtgttc
     3481 gaacggttcg tggcggccta cggtcgcggg cacggcatcg ccgaggatga ggtcaccctg
     3541 gccgaagtcg gcgccctgga ctccctgagc gacatcgtcg gagactgtga ggcgctgcgg
     3601 ctgctcggcg tgacgtcggt gtccgcgtcc ccggtcgccg tcggcgccgg acgcatccac
     3661 accagtcacg gcgatcttcc ggtgcccgtg ccggcggtcg ccgagctggc cctgggctgg
     3721 gggatctcca gaccggcggc cgcgctcgac ggtccggacg cggagggcgc ggccgactgg
     3781 gactcgccgg atccggacgg cgccgtgacc gacaccgccc tggacgtgcg gcacctgccg
     3841 atggccaacg ccaccgtggt cgacgggatg gtcaccgagg gaccggccag atcggtggag
     3901 ccggggcagt tgggcgagtt ggccaccccg accgggatgg cgttgctgcg cggcctggcc
     3961 gagcgctgcg agccgctgcc gccgatgacc cttgcggcac tgggtgtcgg tgcgggtggc
     4021 cgggatgtgc cgggccggcc caacgtcgtc cgggtgctgc tcggttcgcg ctgaagtcgc
     4081 gcgaccgtac cgcgccgcga cctatgctcg gcgcgagcga aggagcacat cgatggagat
     4141 cagcgtccaa ggcacgcacg agcagaccgt ccgcccggag gtggcccggc tgcacgcccg
     4201 cgcgcacgcc gaggcgggcg gcaaggacga ggtgctgcgc acggcgaccg acgtggtgca
     4261 gcagctgtcc ggcgagttga accggttgaa ggaggcgggc gccgtgcgcg aggtggtggt
     4321 gcggccgatc agcacctcgt cgtggcgtcc ggtcgcgcgc ggcaggcagc agccgccgat
     4381 ctaccgcgcc gacgcgagcc tgcaggcgga cttcaccgac ttcgccgcgc tggccgacct
     4441 ggccgcacga ttcggcgcgg tcgacgggtt ggaactgaac tgggtggagt ggcggctgac
     4501 cgacgccacc aggctcgagc ttgaagcggt ctgcctgacc agggccgtcg agcaggcccg
     4561 cgagcgggcc ctggtgatgg cgcgcgcggc gggcgagtct tcggtcgcgt tcgtccaact
     4621 ggcagacccg gggctgctga ccgacaaccc gatccggcgg gaggcgttcg aggcgccgcc
     4681 ggcgatgggc gccatgatgc ggtcggccaa gatggccgac gaactggccg gaatcgagct
     4741 gcagcccgag gatctgaccg tgtcgggtac cgtccaggcc cggttcgtca ccacgcaggg
     4801 gtgagaatga agcgtgccca cctatcgtga cgaagcggtc gtgctgcgca cccacaagct
     4861 gggtgaggcc gaccgcatca tcaccctgct gtcgcggcgc aacggcaaga tccgggcggt
     4921 ggcgaaaggg gtacggcgca ccacgtccaa gttcggtgga cggctggagc cgttcagcca
     4981 catcgatgcc cagttcgcca ccggacgcac cctcgacgtg gtcacgcagg ccgtcaccct
     5041 gcacgcctac accgaaccga tgggcaacga ctacggcgcc tacacctcgg gtcaggtgat
     5101 cctggagacg gctgatcggc tggtcgccga agagcgggaa ccgtcgctgc aacagtttct
     5161 gctgctggtg ggagcactca acgcgatgac cgcgggcacc gcggacgggc cccgtccggc
     5221 caccatgatc atggattcgt acctgctgcg cgccgttgcg gtcgccggct gggcacccag
     5281 cctgtcggac tgcgcgcggt gcggccaggt gggtccgcac gaggcgttca acccgcaggc
     5341 cggcggcatg gtgtgccggc gctgccgccc gcccgcgtcc gcactgccgt cggcagcggt
     5401 gatcggctat ctgtcggcgc tgctcaccgg cgactggccg gccacccgcg aggtggacga
     5461 gcgcgcgcag cgcaccgcca gcggcctggt cagcgccttc gcgaactggc acctggagcg
     5521 gggactgcgc tcgctcatcc acgtcgaccg ataatctggc acgtcgaccg ataatctggc
     5581 ccgatgctgg cggtgaacag gttccgggta gcggacgagc acgccttcgt gccccgggcc
     5641 gcggccgccg tggcctttct cagcgaatgc gacggcaacc tgagcgtcga cctgctgcgc
     5701 aacctcgacg aacccgacct gtggtgcctg gtcagcagct ggcgcaacgt cggctcgtac
     5761 cggcgcagct tcaacggcta caccgccaag ctggtgctgg tgccgctgct cagcgaggcg
     5821 atcgacgaac ccggcgccta cgaccacccc gaggcggtgg gcgagaaccg tccgcgaacc
     5881 cggtaacgtg gtccttcgcg tgccagccgg gcacgcatcg actcacaagg agtttcggtg
     5941 gccagtcgcc tcgacaacgt gatcagcctg tgcaagcgca gaggattcgt ctacccgtgc
     6001 ggtgagatct acggcggcac ccgcgccgcc tgggactacg gcccctacgg cgtggagctg
     6061 aaagagaaca tcaagcggca gtggtggaag gccgtggtgc agggccgcga cgacgtggtc
     6121 ggcatcgact cctcgatcat tctgccccgc caggtgtggg tcgcgtccgg tcacgtgggc
     6181 gtcttctccg acccgctgac cgagtgcctg agctgccaca agcgctttcg cgccgaccag
     6241 ctgatcgaga acttcgagca caagaagggc cgggcaccgt ccgggttgga agagatcaac
     6301 tgccccgagt gcggcaccct gggccgcttc accgagcccc gcgacttcaa catgatgctg
     6361 aagacctacc tcggcgtgat cgagg
//
LOCUS       DAOSUG010000120         6319 bp    DNA     linear   ENV 10-JUN-2024
DEFINITION  MAG TPA_asm: Micropruina sp. isolate AMR_MDS_2195
            NODE_5796_length_6319_cov_4.860792, whole genome shotgun sequence.
ACCESSION   DAOSUG010000120 DAOSUG010000000
VERSION     DAOSUG010000120.1
DBLINK      BioProject: PRJNA925477
            BioSample: SAMN32864262
            Sequence Read Archive: SRR4236673
KEYWORDS    WGS; ENV; Metagenome Assembled Genome; MAG; Third Party Data; TPA;
            TPA:assembly.
SOURCE      Micropruina sp. (wastewater metagenome)
  ORGANISM  Micropruina sp.
            Bacteria; Bacillati; Actinomycetota; Actinomycetes;
            Propionibacteriales; Nocardioidaceae; Micropruina.
REFERENCE   1  (bases 1 to 6319)
  AUTHORS   Abdulkadir,N., Saraiva,J.P., Zhang,J., Stolte,S., Gillor,O.,
            Harms,H. and Rocha,U.
  TITLE     Genome-centric analyses of 165 metagenomes show that mobile genetic
            elements are crucial for the transmission of antimicrobial
            resistance genes to pathogens in activated sludge and wastewater
  JOURNAL   Microbiol Spectr 12 (3), e0291823 (2024)
   PUBMED   38289113
REFERENCE   2  (bases 1 to 6319)
  AUTHORS   Abdulkadir,N.
  TITLE     Direct Submission
  JOURNAL   Submitted (24-MAR-2023) Environmental Microbiology, Helmholtz
            Center for Environmental Research -UFZ, Permoserstr 15, Leipzig
            04318, Germany
COMMENT     The annotation was added by the NCBI Prokaryotic Genome Annotation
            Pipeline (PGAP). Information about PGAP can be found here:
            https://www.ncbi.nlm.nih.gov/genome/annotation_prok/
            
            ##Genome-Assembly-Data-START##
            Assembly Method        :: SPAdes v. 3.11.1
            Genome Representation  :: Full
            Expected Final Version :: Yes
            Genome Coverage        :: Not Applicable
            Sequencing Technology  :: Illumina MiSeq
            ##Genome-Assembly-Data-END##
            
            ##Genome-Annotation-Data-START##
            Annotation Provider               :: NCBI
            Annotation Date                   :: 05/04/2023 04:57:10
            Annotation Pipeline               :: NCBI Prokaryotic Genome
                                                 Annotation Pipeline (PGAP)
            Annotation Method                 :: Best-placed reference protein
                                                 set; GeneMarkS-2+
            Annotation Software revision      :: 6.5
            Features Annotated                :: Gene; CDS; rRNA; tRNA; ncRNA
            Genes (total)                     :: 2,776
            CDSs (total)                      :: 2,750
            Genes (coding)                    :: 2,714
            CDSs (with protein)               :: 2,714
            Genes (RNA)                       :: 26
            tRNAs                             :: 24
            ncRNAs                            :: 2
            Pseudo Genes (total)              :: 36
            CDSs (without protein)            :: 36
            Pseudo Genes (ambiguous residues) :: 0 of 36
            Pseudo Genes (frameshifted)       :: 2 of 36
            Pseudo Genes (incomplete)         :: 32 of 36
            Pseudo Genes (internal stop)      :: 2 of 36
            ##Genome-Annotation-Data-END##
FEATURES             Location/Qualifiers
     source          1..6319
                     /organism="Micropruina sp."
                     /mol_type="genomic DNA"
                     /submitter_seqid="NODE_5796_length_6319_cov_4.860792"
                     /isolate="AMR_MDS_2195"
                     /isolation_source="Activated sludge of municipal
                     wastewater treatment plants"
                     /db_xref="taxon:2737536"
                     /environmental_sample
                     /geo_loc_name="Canada"
                     /lat_lon="49.25 N 123.1 W"
                     /collection_date="2015-06-16"
                     /metagenome_source="wastewater metagenome"
                     /note="metagenomic"
     gene            44..1189
                     /gene="recF"
                     /locus_tag="PLF56_05385"
     CDS             44..1189
                     /gene="recF"
                     /locus_tag="PLF56_05385"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_016668699.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_function: GO:0003697 - single-stranded DNA binding
                     [Evidence IEA];
                     GO_function: GO:0005524 - ATP binding [Evidence IEA];
                     GO_process: GO:0006281 - DNA repair [Evidence IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="DNA replication/repair protein RecF"
                     /protein_id="HOQ53029.1"
                     /translation="MFVTHLSLADFRSYPAAEVPLDPGVTTFTGANGQGKTNLIEAVD
                     YLATLGSHRVSSEQPLVRHGSERAIVRARVQAGLDDARQLLLEVEILPGKQNRASLNR
                     APLRRPRELLGALRTVVFSPEDLALVKGDPGERRRFLDELITTRWPRLAGVRADYDRV
                     LRQRVTLLKSLMGRPRSVRADAESTLEVWDESLARFGAELMHARLTTLADLSPHVAQS
                     YSAIAPVNNDARLDYRCAIGADASTTQAEIEARLILSMGERRGDELQRGVCLVGPHRD
                     DLLLSLGELPAKGYASHGESWSFALSLKLGAFALLRSDGVEPVLILDDVFAELDQMRR
                     DRLAGMIDAAEQVLITAAVPDDVPAALGGRQFRVGGGQVSEVSDARA"
     gene            1176..1670
                     /locus_tag="PLF56_05390"
     CDS             1176..1670
                     /locus_tag="PLF56_05390"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_016454978.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="DciA family protein"
                     /protein_id="HOQ53030.1"
                     /translation="MPEHDPTGIELARLIAASAQGGPATPRPAKAVSAPKKPKPAKQR
                     PAGDPQPLGEALDDLIQRSGWSTEVNLHHLLGRWSDLVGPTNAEHSTPEGFANQVLQV
                     RTDSTAWATNLRLLAPSIVARLNDQLGDGTVLRIVVKGPDAPSWKHGLRSVRDGRGPR
                     DTYG"
     gene            1797..3137
                     /locus_tag="PLF56_05395"
     CDS             1797..3137
                     /locus_tag="PLF56_05395"
                     /inference="COORDINATES: ab initio
                     prediction:GeneMarkS-2+"
                     /note="Derived by automated computational analysis using
                     gene prediction method: GeneMarkS-2+."
                     /codon_start=1
                     /transl_table=11
                     /product="hypothetical protein"
                     /protein_id="HOQ53031.1"
                     /translation="MTAVRTMLHYLNQLVTTTGLVWWRCLPRLLITAMLGWLGYRIFA
                     QLAAILVDVSAWWSLLMMSMGFVVRLSAIIIGLRVAGEELQIRESIPLPQDDPRDASV
                     SHLLSRTLLPFLGIYAVFDTVTDAANKIEVDYYVFHGLTFERPVLSQLSPQGAGVGVI
                     IGVIVGLYVARRLVDWWFERTGFRPLGLVAALIEGFFLLIVILSGRQVLISVSDWVDG
                     RNFRLWLDYPGWGVRQLASWLGIDILGVLDRLGELWWNTIWPAISAMVVEPMLWLALA
                     SLVYGTQVLSTADLWRKGRPVTVHTRRSQLQVGDRQRRLALEFQEAFFGDLDDKYLPA
                     FQSLRLVLSVGAPFFAAFILCYGMLTTGEEWLSRAIYHAIGGHPATFWAVAGPVLELP
                     IEMLVQPLRWVLLATSFRACVALFAARAGQPEWSPDSLAPDRSADQTAATEVRS"
     gene            3134..3694
                     /locus_tag="PLF56_05400"
     CDS             3134..3694
                     /locus_tag="PLF56_05400"
                     /inference="COORDINATES: ab initio
                     prediction:GeneMarkS-2+"
                     /note="Derived by automated computational analysis using
                     gene prediction method: GeneMarkS-2+."
                     /codon_start=1
                     /transl_table=11
                     /product="hypothetical protein"
                     /protein_id="HOQ53032.1"
                     /translation="MTRRLSGWLVALVTCVAMVALHAYTGEFPHSSVQQVAIRQTGRL
                     YGSTITVNSWRIGQVLYEDDAFVGRSGVMFLAVNLTLATDSAREPSSRWQVGGTSNGH
                     TLAPREGVRPPQPGFTRTQDVVFELNPDDLAGFTLTVLDRAPIYAFDPELWIDLGITP
                     QQAADELVRNRYATVKASEGTVEVIR"
     gene            3691..4221
                     /locus_tag="PLF56_05405"
     CDS             3691..4221
                     /locus_tag="PLF56_05405"
                     /inference="COORDINATES: ab initio
                     prediction:GeneMarkS-2+"
                     /note="Derived by automated computational analysis using
                     gene prediction method: GeneMarkS-2+."
                     /codon_start=1
                     /transl_table=11
                     /product="hypothetical protein"
                     /protein_id="HOQ53033.1"
                     /translation="MTERVRQWLRAAIVFTLVVAVAGAWLGYINARNTPHYRQTEPGA
                     VAVPDQNSRLQLRLVSLVTTATMVTSRDSAHAPAGALYVVAVVEYVPDPDGSLCWLDL
                     LAVDGRRWATTELTEVSGERELPTSCSSEPVNGTPRAELIYLVPEQAVNSLAGLVSSS
                     TAYRTTDPYWVLTPPG"
     gene            complement(4388..5056)
                     /locus_tag="PLF56_05410"
     CDS             complement(4388..5056)
                     /locus_tag="PLF56_05410"
                     /inference="COORDINATES: ab initio
                     prediction:GeneMarkS-2+"
                     /note="Derived by automated computational analysis using
                     gene prediction method: GeneMarkS-2+."
                     /codon_start=1
                     /transl_table=11
                     /product="hypothetical protein"
                     /protein_id="HOQ53034.1"
                     /translation="MTEPPLPAVQADHEPDGDAHVGPSGGVDDAMGAPSRSIPVLVII
                     AAVVLALAGGLWWAGGFDEAKGRLFRLPAGTVVNMGPMSVAFDRALAREQFGAWTLYV
                     FGRCQNNTDAALDPTKDRLVRNGISAQHPITKELPDMILLYFGPGTTLGHSGVLNPGT
                     PMVPCQLAITFKSFPDTDFVSVGCSEMEWIDSSPTGDGTMVWSAARVGYRFEVPVVIE
                     RDQD"
     gene            5424..>6319
                     /locus_tag="PLF56_05415"
     CDS             5424..>6319
                     /locus_tag="PLF56_05415"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_002518559.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="ATP-binding protein"
                     /protein_id="HOQ53035.1"
                     /translation="MSEPTNGHRTVGSAAHAVDAGSYEASAITVLEGLEAVRKRPGMY
                     IGSTGERGLHHLVQEVVDNSVDEALAGYCDTIIVRLLDEGGVQVIDNGRGIPVAEHPK
                     EKISALTVALTMLHAGGKFGDGGYKVSGGLHGVGVSVVNALSTHLTADVRRDGYHWQQ
                     SFSLGDPDAPLERLEPATDTGTTITFYASPDIFETTTYNYETLATRFREMAFLNKGLT
                     ITLIDERSERRTDEDDEPHSDTFRFDEGLIDFVKFLTGSKETIHPSVIAIDATNDESK
                     LSLEVAMQWNSSFSESVHTFANT"
BASE COUNT          997 a         2100 c         2192 g         1030 t
ORIGIN      
        1 tgcgtcagcc gccgcgcctc gcccggtgtc gtagggtgac ggcgtgttcg tcacccacct
       61 ctcactggcc gattttcggt cgtacccggc ggccgaggtg ccgctggatc ccggggtcac
      121 gacgttcacc ggggccaacg ggcagggcaa gacgaacctg atcgaagcgg tcgactacct
      181 ggccacgctg gggtcgcacc gggtgtcgtc cgagcagccg ctggtgcggc atggcagcga
      241 gcgcgcgatc gtgcgggcac gggtacaggc cggcctggac gatgcgcggc agctgctgct
      301 cgaagtcgag atccttcccg gcaagcagaa ccgggccagc ctcaaccggg caccgttgcg
      361 gcggccgcgc gaactgctcg gcgccctgcg caccgtggtg ttctcgccgg aggatctggc
      421 tctggtcaag ggtgatccgg gcgagcggcg gcgctttctg gacgagctga tcaccacccg
      481 ttggccgcgc ctggcaggcg tccgtgccga ctatgaccgg gtgctgcggc aacgggtgac
      541 gctgctgaag tcgctgatgg gacgcccgcg cagcgtgcgg gccgacgccg aatcgacgct
      601 agaagtgtgg gacgaatccc tggcccggtt cggtgccgag ttgatgcatg cccggctcac
      661 cacgctggcg gatctgagcc cgcacgtggc gcagtcctac agcgcgatcg cgccggtcaa
      721 caacgacgcc cggctggact accgctgcgc gatcggtgcc gacgcgtcca ccacgcaggc
      781 cgagatcgag gcgcggctga ttctgtccat gggggagcgg cggggcgacg aactgcagcg
      841 cggcgtctgc ctggtcggtc cgcaccgcga cgaccttctg ctgagcctcg gcgagctacc
      901 cgccaagggg tacgcgtccc acggcgagtc gtggtcgttc gccctgtcgc tcaagctcgg
      961 tgccttcgcg ctgctgcggt ccgacggggt cgaaccggtg ctgattctcg acgacgtgtt
     1021 cgccgagctg gaccagatgc gccgcgaccg gctggcgggc atgatcgatg cagccgaaca
     1081 ggtgctgatc accgccgctg tgccggacga tgtgccggcc gccctgggcg gacgacagtt
     1141 ccgggtgggc ggcggccagg tgagcgaggt gagcgatgcc cgagcatgac ccgaccggga
     1201 tcgagctggc ccggctgatc gccgccagcg cgcagggcgg gccggccacc cctcgtccgg
     1261 ccaaggctgt gtccgccccg aagaagccga agcccgccaa gcagcggccg gctggcgacc
     1321 cgcaacccct gggcgaggcc ctggacgacc tgatccaacg ctccggctgg agcaccgagg
     1381 tgaacctgca ccacctgctg ggccgctggt ccgacctggt cgggccgacg aacgccgagc
     1441 actccacacc ggagggtttt gccaaccagg tgctccaggt gcgcaccgac tccaccgcct
     1501 gggcgaccaa cctgcggttg ctggcgccgt ccatcgtggc ccggctgaac gaccagctgg
     1561 gcgacggcac cgtgctgcgc atcgtggtga aaggcccgga cgccccgagc tggaagcacg
     1621 gtctgcgcag tgtgcgcgac gggcgagggc cgcgcgacac ctacggctga gctgagcctg
     1681 gaccccgggc tgagggtagt ccggggcacg gcggtgagtg cgacgccggc cgcggggacg
     1741 gtggcgtggg attgcctcgg acggcgtgac caccccggcg gttaggatcg cccacgatga
     1801 cggcggtacg aaccatgctc cactacctga accaactggt caccacgacc gggctggtgt
     1861 ggtggcgctg cctgcccagg ctattgatca ccgccatgct cggctggctc gggtaccgca
     1921 tcttcgccca gcttgccgcc attctggtcg acgtcagtgc gtggtggtcg ttgctgatga
     1981 tgtcgatggg cttcgtggtg cgactgtccg cgatcatcat cggcctgcgg gtcgcgggcg
     2041 aggaactgca gatccgcgaa tcgatcccgc tgccgcagga cgatccgcgc gatgcgtccg
     2101 tttcgcatct gctctcgcgc acgctgctgc catttctggg catctacgcc gtcttcgaca
     2161 cggtcaccga tgccgcaaac aagatcgagg tcgactacta cgtgtttcac gggttgacgt
     2221 tcgaacgtcc ggtgctcagc caactgagtc cgcagggtgc cggggtcggg gtgatcatcg
     2281 gggtgatcgt cggcctgtat gtggcgcgcc ggctggtcga ttggtggttc gaacgcaccg
     2341 gctttcggcc gttgggtctg gtcgcagccc tgatcgaggg cttctttctg ctgatcgtga
     2401 tcctgagcgg acgtcaggta ctgatctcgg tgtccgactg ggtggacggg cgcaacttcc
     2461 ggctctggct cgactacccg ggctgggggg tgcgacagct ggcatcctgg ctcggcatcg
     2521 acatcctggg cgtgctggat cggctcggtg agctgtggtg gaacaccatc tggccggcca
     2581 tcagcgcgat ggtggtggaa ccgatgttgt ggctggccct ggcttcgctg gtgtacggca
     2641 cccaggtgct ttccaccgcc gacctgtggc gcaagggccg tccggtgacc gtgcacacgc
     2701 gacgatcgca gctgcaggtc ggcgatcgcc agcgccggct cgcgctggag ttccaggagg
     2761 cgttcttcgg cgacctggac gacaagtacc tgccggcgtt ccagtccctg cggctggtgt
     2821 tgagtgtcgg cgcacccttc ttcgccgcgt tcatactctg ctacggcatg ctgaccaccg
     2881 gcgaagagtg gctcagccgg gccatttacc acgctattgg tgggcacccg gccacgttct
     2941 gggccgtcgc cggaccggtg ctcgagctgc cgatcgagat gctggtgcag ccgctgcggt
     3001 gggtgctgct ggccacctcg ttccgggcct gcgtcgcatt gttcgcagcc cgcgccggcc
     3061 aacccgagtg gagccccgac tcccttgcgc cggaccgatc agccgaccag accgccgcga
     3121 cggaggtgcg ttcgtgaccc ggcggctgag tggttggctg gtggcgctgg tcacctgcgt
     3181 ggccatggtg gccctgcacg cctacaccgg tgagtttcca cactcgagcg tgcaacaggt
     3241 cgcgatccga cagaccgggc ggctgtacgg atcgacgatc acggtcaact cgtggaggat
     3301 cggacaggtg ctctatgagg acgacgcctt cgtcggacgc agtggtgtga tgtttctggc
     3361 ggtcaacctc accctcgcca ccgattcggc ccgggagccg agcagccgct ggcaggtggg
     3421 tggaacgtcc aacgggcaca ccctggcccc gagggagggc gtgcgcccac cgcagccggg
     3481 gttcacccgc acgcaggacg tggtcttcga gctgaacccg gacgacctgg ccggcttcac
     3541 tttgacggtg ctcgaccgtg ccccgatcta cgccttcgac cccgagctgt ggatcgacct
     3601 cggcatcacg ccgcagcagg ccgccgacga gcttgtgcgg aaccgttacg ccaccgtgaa
     3661 ggcgtccgag ggaacagtcg aggtgatccg gtgaccgagc gggtccgcca gtggctgcgg
     3721 gctgcgatcg tctttacctt ggtcgtggcc gtggcgggcg cctggctggg ctacatcaac
     3781 gcccgcaaca cgccgcacta ccggcagacg gaaccgggag cagtggccgt cccggaccag
     3841 aacagcaggc tgcaactgcg tctggtgtca ctggtcacca ccgccaccat ggtcaccagc
     3901 agggactcgg cccacgcgcc ggccggtgcg ctctacgtcg tggcggtggt cgagtacgtg
     3961 cccgaccccg atgggagtct gtgttggctc gatctgctgg cggttgacgg acggcggtgg
     4021 gccacgactg aactgaccga ggtgtcgggg gagcgtgaac tgcccaccag ttgctccagc
     4081 gagccggtca acggaacgcc acgagcggag ttgatctacc tggtgccgga acaggccgtg
     4141 aactcactgg ccggactggt cagcagcagc accgcctatc gcacgacgga cccctactgg
     4201 gtgctgaccc cgcctggctg atcgggccgc gggcgcggtg aacggcccga ccgggatccg
     4261 agagcgggct gagcagttcg ccgggcaacg caccttccgt tggttgagcg gtcgaaaccc
     4321 tgtcgaacga gtctcgacac gcgcgacagc gtgaatcggt ggatcagggc tcagcgtgga
     4381 tccaggctca gtcctggtcg cgctcaatca ccaccggtac ctcgaagcga tagcccaccc
     4441 gggcggccga ccacaccatg gtgccgtcgc cggtggggct ggagtcgatc cactccatct
     4501 cggagcaacc gaccgagacg aagtcggtgt cggggaacga cttgaaggtg atcgccagtt
     4561 ggcacggcac catcggggtg ccggggttga gtacgccgga gtggcccaag gtggttcccg
     4621 ggccgaagta cagcaagatc atgtccggca actctttggt gatcgggtgc tgggccgaga
     4681 tgccgttgcg caccaaccgg tccttggtcg gatcgagggc ggcgtcggtg ttgttttggc
     4741 agcgcccgaa gacgtacagc gtccaggcac cgaactgttc gcgggccaac gcccggtcga
     4801 aggcgaccga catcgggccc atgttcacca cggtgcccgc gggcagccgg aacaatcgtc
     4861 ccttggcctc gtcgaagccg ccggcccacc acagcccgcc ggctagggcc agcaccaccg
     4921 ctgcgatgat caccagcacc gggatcgagc ggctcggggc acccatcgcg tcgtccacac
     4981 cgccggacgg acccacgtgg gcgtcgccgt cgggctcgtg gtcggcctgg acggccggca
     5041 gcggcggctc ggtcatggcg ggagtttagt tcagggggtg tcgcataccc cgggccggac
     5101 tgccgtgcac cccacgcggt cgctcgctgc gttgcgctgc tcgccccgac gtccagtacc
     5161 ggcctcgtgg gcgtcagcga ctcagcgccg tgggaccgtg cccacatcct ggggtatggg
     5221 gggccgtgga tgggggcaaa gcggctcaga gggccatttg cggcccttct gtccacaatt
     5281 tgtgctcgga cagaccttca gattggggcc aacaggggcg aacagggtag actggaccag
     5341 ttgcagacac ctcggcacgc gggtccccgg atcgtgggcg gtgagtgtct gactgtgacc
     5401 cgaacagcaa ggggtaacca ccagtgagtg aaccgaccaa cggtcaccga acggtcggct
     5461 cggcggcgca cgccgtcgac gccggcagct atgaagccag tgccatcacg gtgctcgagg
     5521 gtctcgaggc cgtccgcaag cgaccgggca tgtacatcgg ctccaccggt gagcgaggtt
     5581 tgcaccacct ggtgcaagag gtggtcgaca actccgtcga tgaggcgctg gccggctact
     5641 gcgacacgat catcgtgcgg ctgctcgacg aaggcggcgt gcaggtgatc gacaacggcc
     5701 gcggcattcc ggtggccgag caccccaaag agaagatttc ggccctgacc gttgcgctca
     5761 ccatgctgca cgccggcggc aagttcggcg acggcggcta caaggtctcc ggcggtctgc
     5821 acggtgtggg cgtgtcggtg gtgaacgcgc tgtccaccca cctgaccgcc gacgtgcgcc
     5881 gcgacggcta ccactggcag cagtcgttct cgctcggcga cccggacgct ccgcttgagc
     5941 gtctcgaacc cgccaccgac accggcacga cgatcacctt ctacgccagc cccgacattt
     6001 tcgagaccac cacctacaac tacgagaccc tggccacccg gttccgcgag atggcgttcc
     6061 tgaacaaggg cctgaccatc acgctgatcg acgaacgctc cgaacggcgc accgacgaag
     6121 acgacgagcc gcacagcgac acgttccggt tcgacgaggg cctgatcgac ttcgtgaagt
     6181 tcctgaccgg cagcaaagag accatccacc cctccgtgat cgcgatcgac gccaccaacg
     6241 acgaatccaa gctcagcctc gaggtcgcga tgcagtggaa cagctcgttc agcgagagcg
     6301 tgcacacctt cgccaacac
//
LOCUS       DAOSUG010000121         6305 bp    DNA     linear   ENV 10-JUN-2024
DEFINITION  MAG TPA_asm: Micropruina sp. isolate AMR_MDS_2195
            NODE_5811_length_6305_cov_4.213120, whole genome shotgun sequence.
ACCESSION   DAOSUG010000121 DAOSUG010000000
VERSION     DAOSUG010000121.1
DBLINK      BioProject: PRJNA925477
            BioSample: SAMN32864262
            Sequence Read Archive: SRR4236673
KEYWORDS    WGS; ENV; Metagenome Assembled Genome; MAG; Third Party Data; TPA;
            TPA:assembly.
SOURCE      Micropruina sp. (wastewater metagenome)
  ORGANISM  Micropruina sp.
            Bacteria; Bacillati; Actinomycetota; Actinomycetes;
            Propionibacteriales; Nocardioidaceae; Micropruina.
REFERENCE   1  (bases 1 to 6305)
  AUTHORS   Abdulkadir,N., Saraiva,J.P., Zhang,J., Stolte,S., Gillor,O.,
            Harms,H. and Rocha,U.
  TITLE     Genome-centric analyses of 165 metagenomes show that mobile genetic
            elements are crucial for the transmission of antimicrobial
            resistance genes to pathogens in activated sludge and wastewater
  JOURNAL   Microbiol Spectr 12 (3), e0291823 (2024)
   PUBMED   38289113
REFERENCE   2  (bases 1 to 6305)
  AUTHORS   Abdulkadir,N.
  TITLE     Direct Submission
  JOURNAL   Submitted (24-MAR-2023) Environmental Microbiology, Helmholtz
            Center for Environmental Research -UFZ, Permoserstr 15, Leipzig
            04318, Germany
COMMENT     The annotation was added by the NCBI Prokaryotic Genome Annotation
            Pipeline (PGAP). Information about PGAP can be found here:
            https://www.ncbi.nlm.nih.gov/genome/annotation_prok/
            
            ##Genome-Assembly-Data-START##
            Assembly Method        :: SPAdes v. 3.11.1
            Genome Representation  :: Full
            Expected Final Version :: Yes
            Genome Coverage        :: Not Applicable
            Sequencing Technology  :: Illumina MiSeq
            ##Genome-Assembly-Data-END##
            
            ##Genome-Annotation-Data-START##
            Annotation Provider               :: NCBI
            Annotation Date                   :: 05/04/2023 04:57:10
            Annotation Pipeline               :: NCBI Prokaryotic Genome
                                                 Annotation Pipeline (PGAP)
            Annotation Method                 :: Best-placed reference protein
                                                 set; GeneMarkS-2+
            Annotation Software revision      :: 6.5
            Features Annotated                :: Gene; CDS; rRNA; tRNA; ncRNA
            Genes (total)                     :: 2,776
            CDSs (total)                      :: 2,750
            Genes (coding)                    :: 2,714
            CDSs (with protein)               :: 2,714
            Genes (RNA)                       :: 26
            tRNAs                             :: 24
            ncRNAs                            :: 2
            Pseudo Genes (total)              :: 36
            CDSs (without protein)            :: 36
            Pseudo Genes (ambiguous residues) :: 0 of 36
            Pseudo Genes (frameshifted)       :: 2 of 36
            Pseudo Genes (incomplete)         :: 32 of 36
            Pseudo Genes (internal stop)      :: 2 of 36
            ##Genome-Annotation-Data-END##
FEATURES             Location/Qualifiers
     source          1..6305
                     /organism="Micropruina sp."
                     /mol_type="genomic DNA"
                     /submitter_seqid="NODE_5811_length_6305_cov_4.213120"
                     /isolate="AMR_MDS_2195"
                     /isolation_source="Activated sludge of municipal
                     wastewater treatment plants"
                     /db_xref="taxon:2737536"
                     /environmental_sample
                     /geo_loc_name="Canada"
                     /lat_lon="49.25 N 123.1 W"
                     /collection_date="2015-06-16"
                     /metagenome_source="wastewater metagenome"
                     /note="metagenomic"
     gene            <1..444
                     /gene="manB"
                     /locus_tag="PLF56_05420"
     CDS             <1..444
                     /gene="manB"
                     /locus_tag="PLF56_05420"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_013864779.1"
                     /note="converts mannose-6-phosphate to
                     mannose-1-phosphate; the resulting product is then
                     converted to GDP-mannose by ManC which is then used in the
                     synthesis of mannose-containing glycoconjugates that are
                     important for mediating entry into host cells; Derived by
                     automated computational analysis using gene prediction
                     method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="phosphomannomutase/phosphoglucomutase"
                     /protein_id="HOQ53036.1"
                     /translation="HTSVKALMAKHHAIFGGEHSAHYYFRDFWGADTGMLAALHVLAQ
                     LGRSSGTMSELVARLPSFAASGELNSTVADVPAVIDRVAAAFEGRGTHDRSDGLTIET
                     DDWWVNVRPSNTEPLLRLNVEARTPELMASLRDEALAVIRGGSDA"
     gene            437..622
                     /locus_tag="PLF56_05425"
     CDS             437..622
                     /locus_tag="PLF56_05425"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_015071074.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="Trm112 family protein"
                     /protein_id="HOQ53037.1"
                     /translation="MPDLTHDVLELLVCPACHSGFTWDYEASELVCTGADCGLAYPLR
                     GGIPILLIDEARRPARG"
     gene            627..1640
                     /locus_tag="PLF56_05430"
     CDS             627..1640
                     /locus_tag="PLF56_05430"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_020576903.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_function: GO:0004347 - glucose-6-phosphate isomerase
                     activity [Evidence IEA];
                     GO_function: GO:0004476 - mannose-6-phosphate isomerase
                     activity [Evidence IEA];
                     GO_process: GO:0005975 - carbohydrate metabolic process
                     [Evidence IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="SIS domain-containing protein"
                     /protein_id="HOQ53038.1"
                     /translation="MFDFDDSRLENAEVLADASPLLMNLASSGARVRREAALAEGQLA
                     ALADSDRPRAVIAYGPEARLLRSVLEPVCPVPFVAWPSLGLPGWVGPLDVVLLIGRRG
                     PESLAVAYEAVRRGARLIAVCPSDSPLAKQAASRSTTLLPVNTGDPLAAAVVALVALH
                     RLGLGPATNPDAIAAAMDLVAENCSARTDVSVNPAKAMALDLAEAAPLVWGGSVLAAR
                     ASRRIVEALRLASGHIGLSADSADLVSLIQQVRPRDPFADPFDEGAAERRPGLVLVDD
                     GLDDEASAEARLRLQRTAEAVDIRVTRLTHTTDSVMERYATLLLTGLFGAAYLQIGLG
                     RVA"
     gene            1766..2212
                     /gene="aroQ"
                     /locus_tag="PLF56_05435"
     CDS             1766..2212
                     /gene="aroQ"
                     /locus_tag="PLF56_05435"
                     /EC_number="4.2.1.10"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_016665933.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_function: GO:0003855 - 3-dehydroquinate dehydratase
                     activity [Evidence IEA];
                     GO_process: GO:0009423 - chorismate biosynthetic process
                     [Evidence IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="type II 3-dehydroquinate dehydratase"
                     /protein_id="HOQ53039.1"
                     /translation="MTSLEPQRVLVLNGPNLGRLGTREPDVYGAVTHAQLAEHLVGTG
                     AGLGLSVEVRQTDSEGDMIGWLHEAADTDVPVVLNPAAWTHYSIAIADAVAQVPIVIE
                     VHISNVHAREQFRRHSVVSPYALGVIAGLGLGGYDLALAHLAAIRD"
     gene            2231..2908
                     /locus_tag="PLF56_05440"
     CDS             2231..2908
                     /locus_tag="PLF56_05440"
                     /inference="COORDINATES: protein motif:HMM:NF019151.2"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="HNH endonuclease family protein"
                     /protein_id="HOQ53040.1"
                     /translation="MLRRRRWGRALAVAATVLLVLGAIIGAVVWVAPHPPEGVQVVPD
                     ASIVTVPGYERGCKAGEGCVFGPAWSDDVAVAGGHNGCDTRNDLLNATLSNRVHRPGT
                     RECVVLAGDFVDPYTGRQVHFTKQNADQVPVDHVFALAAAWNRGAAEWTPQRRRDFAN
                     DPLNLVVTTASANLAKRDRTPATWLPESPAGQCLFVSRFVEVARSYQLPISSSDLRVI
                     DRVQRGC"
     gene            complement(3004..3498)
                     /locus_tag="PLF56_05445"
     CDS             complement(3004..3498)
                     /locus_tag="PLF56_05445"
                     /inference="COORDINATES: protein motif:HMM:NF012521.2"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="histidine phosphatase family protein"
                     /protein_id="HOQ53041.1"
                     /translation="MTRTLIVMRHAKSSWGTSEPDHQRPLNKRGRQDALAAGAILADY
                     PLDAVLSSSATRTRQTWQRAAEGGAQCADVEFTDALYGAWTDAVIDLAGELEDSVSTA
                     LLLGHEPTVSSVVHRLAEPSELTDQAAAGFTTCAMAFLEFDGTWAELAPGRARLVRFE
                     VPRG"
     gene            complement(3502..3759)
                     /locus_tag="PLF56_05450"
     CDS             complement(3502..3759)
                     /locus_tag="PLF56_05450"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_014846041.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="DUF3039 domain-containing protein"
                     /protein_id="HOQ53042.1"
                     /translation="MTELAPGAQTILDERTEQRVDPGDHERFSHYVPKNKLTEAMVMG
                     TPVVALCGKVWVPSRNPDRFPVCPECKEIWASMRPGGDDES"
     gene            4057..5100
                     /locus_tag="PLF56_05455"
     CDS             4057..5100
                     /locus_tag="PLF56_05455"
                     /inference="COORDINATES: protein motif:HMM:NF012640.2"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_function: GO:0046872 - metal ion binding [Evidence
                     IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="anthranilate synthase component I family
                     protein"
                     /protein_id="HOQ53043.1"
                     /translation="MAGVQRYGPDEPLAVVGGRLLTGLREVTADLTALDSAGFWAVVL
                     PFEGPAICARFERVRPAPPWRGRPWLGPPRDAWRSSLDEAGFAAGVRHIRDAIAAGGV
                     YQVNLTRRLSAPLPSRTSIAALGAALAQGNPAPFSAVVHLPAQGVHVASASPELFLSR
                     RGEVVWSSPIKGTAKGVDDFLAKDAAENVMIVDLVRNDLGRVCRPGSVEVPSLLAVEP
                     HPGLFHLVSTVRGRLRARIGWADLITATFPPGSVTGAPKLAALDVIAQLEPVGRGPYC
                     GAIGWVDAGRVRGELNVAIRTFWIGDDHLHLGVGGGITWDSSASGEWAETELKAANLL
                     RVASRSAVHPRGG"
     gene            5200..>6305
                     /locus_tag="PLF56_05460"
     CDS             5200..>6305
                     /locus_tag="PLF56_05460"
                     /inference="COORDINATES: protein motif:HMM:NF019310.2"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_function: GO:0022857 - transmembrane transporter
                     activity [Evidence IEA];
                     GO_process: GO:0055085 - transmembrane transport [Evidence
                     IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="MFS transporter"
                     /protein_id="HOQ53044.1"
                     /translation="MSDTVNRRGLSVGLMLCVMAISFESYAVLTAMPAAARDLGNVGL
                     YAWTFTAFVIAMTFSIVASGQFTDRYGPVQPMMVGFGVFAAGLVIAALSTSMWVLLVA
                     RFTQGLGAGTMNVAVMVLIARAYEESDRAKLMTWFSACWMIPAFVGPPLAGWIVQISS
                     WHFVFWSVLPLMAVGGLLVLRPLRGIELTPEEPHELKKRLMLLAAAVAAGLALIQWAG
                     QSLNLWSALWLVVGLAAIVFGLPPLMPKGIDWTGKGISGVVGARLFSAGAFFGAQSFL
                     TLMLIEERGLDAVFTGLTLTIGSLGWMSGALLQAQSWLKWRRDQMILVGVAAITAGIA
                     LLAVAAYIPQSWIGLVMIAWIIAGFGMGLQNPSTS"
BASE COUNT          836 a         2160 c         2260 g         1049 t
ORIGIN      
        1 cacacctcgg tcaaggcgct gatggcgaag caccacgcga tcttcggcgg cgagcattct
       61 gcccactact actttcgcga cttctggggg gccgacaccg gcatgctggc cgccctgcac
      121 gtgttggccc agctcggtcg aagcagcggc accatgtccg aactggtcgc ccgcttgccg
      181 tcgttcgcgg cctccggcga gctcaactcg accgtcgcgg acgtgcccgc ggtgatcgat
      241 cgggtcgccg ccgcattcga gggccgcggc acccatgacc gctccgacgg gctcaccatc
      301 gaaaccgatg actggtgggt gaacgtccgg ccgtccaaca ccgaacccct gctgcgactc
      361 aacgtcgagg cgcggacgcc cgagctgatg gcgtccctgc gcgacgaggc cctggccgtc
      421 atccgaggag gatccgatgc ctgacctgac ccatgacgtg ctcgaactgc tggtctgccc
      481 ggcctgccac agtggtttca cctgggacta cgaggcgtcc gaactggtct gcaccggcgc
      541 cgactgcggc ctggcgtacc cgctgcgcgg cggcataccg atcctgctga tcgacgaagc
      601 ccgccggccg gcccggggct gagccgatgt tcgacttcga cgactcccgg ctggagaacg
      661 ccgaggtgct ggccgatgcc tcgccgttgc tgatgaacct ggccagttcg ggcgcgcggg
      721 tgcggcgtga ggccgcgctc gccgaggggc agttggcggc gctcgccgat tcggaccgtc
      781 cccgtgcggt gatcgcctac ggccccgagg cgcggctgct gcgctcggtg ctcgaaccgg
      841 tctgcccggt tccgttcgtc gcgtggccct cgctcggcct gcccggctgg gtgggtccgc
      901 tggacgtggt gctgctgatc ggacggcgcg gtcccgagag cctcgcggtg gcctacgagg
      961 cggtacgacg gggcgctcgg ctgatcgccg tctgcccgtc cgactcgcca ctggccaagc
     1021 aagccgcgtc acggtccacc accctgctcc cggtcaacac cggtgacccg ctggccgcgg
     1081 ccgtggttgc gctggtggcc ctgcaccggc tggggctcgg ccccgccacc aatccggacg
     1141 cgatcgccgc cgccatggac ctggtcgccg agaactgctc ggctcgcacc gacgtctcgg
     1201 tcaacccggc caaggccatg gcactcgatc tggccgaggc ggcaccgctg gtctggggcg
     1261 ggtcggtgct ggcggcccgc gccagccggc gcatcgtcga ggcgttgcgg ttggcgtccg
     1321 ggcacatcgg gttgtcggcc gattccgccg acctggtcag cctgatccag caggtgcgtc
     1381 cacgagaccc tttcgccgat ccgttcgacg agggtgcggc cgagcggcgt ccgggcctgg
     1441 tgctggtgga cgacggtctg gacgacgagg cctccgccga ggccaggttg cggctgcagc
     1501 gcaccgccga ggcggtcgat attcgggtca cccggctgac gcacaccacc gattcggtga
     1561 tggagcgcta cgcgaccctg ctgctcaccg gcctgttcgg tgccgcctac ctgcagatcg
     1621 ggctggggcg cgtcgcctag cccggcccac ccaacgcctc cctcaccatc tctggcccgc
     1681 gtagctacgc gggccagaga tggtgagggg ggcgttgaac ccagaggggc ggcgtaggct
     1741 cggtcgggct ggcacaatga ccggcgtgac cagcctcgaa ccgcaacgcg tccttgttct
     1801 gaacgggccc aacctgggtc ggctgggcac ccgtgaaccg gacgtctacg gtgccgtcac
     1861 ccacgcccaa ctcgccgagc atctggtcgg cacgggtgcg ggtctcgggt tgtcggtcga
     1921 ggtgcgacag accgacagcg agggcgacat gatcggctgg ctgcacgagg ccgccgacac
     1981 ggacgttccg gtggtgctca acccggccgc ctggacccac tactcgatcg cgatcgcgga
     2041 cgccgtggcg caggtgccga tcgtgatcga ggtgcacatc agcaacgtgc acgcccgcga
     2101 gcagttccgc aggcactccg tggtgtcgcc gtacgcgctg ggcgtgatcg ctggtctcgg
     2161 cctcggcggg tacgacctgg ccctggcgca tctcgcagcg attcgtgact gagcgccgtc
     2221 ccgataaccc atgctgagac gccggcgatg gggccgggcg ctcgccgtcg ccgccaccgt
     2281 gctgctggtg ctgggtgcga tcatcggcgc cgtggtgtgg gtcgcgcccc acccacccga
     2341 gggcgtgcag gtggtgccgg atgcgagcat cgtcaccgtt cccggctacg agcgcggctg
     2401 caaggccggt gagggttgcg tgttcgggcc ggcctggagc gacgacgtgg ccgtcgccgg
     2461 tggacacaac gggtgcgaca cccgcaatga cctgctgaac gccacgctgt ccaatcgcgt
     2521 ccaccgcccc ggcacccgcg aatgcgtggt gctcgccggc gatttcgtcg acccctacac
     2581 cggccggcag gtgcatttca ccaagcagaa cgccgatcag gtgccggtcg accatgtgtt
     2641 cgcactcgcg gccgcctgga accgcggtgc cgccgagtgg acgccgcagc gccgccgcga
     2701 cttcgccaac gacccgctga acctggtggt caccaccgcg tccgccaacc tggccaaacg
     2761 cgaccggacg cctgccacct ggctgcccga gtcgcccgcc ggtcagtgcc tcttcgtcag
     2821 ccggttcgtc gaggtggcgc gcagctacca gctgccgatc agcagctccg acctgcgcgt
     2881 gatcgaccgg gtgcaaaggg gctgctgacg gcgagcgcgc ggtgtccgcg cgcggacgcg
     2941 tgagccgtgc cgcgtccttc atccgaggtg acggcgcctc ccgtccggga cagggtgcag
     3001 aactcagccg cgcggcacct cgaagcgcac cagccgcgcg cgtccggggg cgagttcggc
     3061 ccaggtgccg tcgaactcca gaaacgccat ggcgcaggtt gtgaaaccgg ccgcggcctg
     3121 atccgtcagc tccgacggct cggccagccg gtgcaccacc gaactgaccg tcggctcgtg
     3181 gcccagcagc aatgcggtgc tcaccgaatc ctccagttcg ccggccaggt cgatcaccgc
     3241 gtcggtccac gcgccgtaca gggcatcggt gaactcgacg tccgcgcact gggcgccgcc
     3301 ctcggcggcc cgctgccagg tctgccgggt gcgggtcgcc gacgatgaga gcaccgcgtc
     3361 cagcgggtag tcggccagaa tggcgccggc cgccagggcg tcctgccgtc cgcgcttgtt
     3421 caacggacgc tgatggtcgg gctcggacgt gccccacgac gacttggcgt gccgcatcac
     3481 gatcaaggtg cgggtcatgg ctcaggactc gtcgtcgccg ccgggccgca tggaggccca
     3541 gatctctttg cattcggggc acaccggaaa cctgtcgggg ttgcggctcg gtacccagac
     3601 cttgccgcac agcgccacca ccggcgtgcc catcaccatg gcttcggtca gcttgttctt
     3661 gggcacgtag tggctgaacc gttcgtgatc gcccgggtcg acccgctgct cggtgcgttc
     3721 gtcgaggatc gtctgtgcgc cgggtgccag ttcggtcatg ccgccgactc tagcctggat
     3781 ccaccgatgg gcgccgtcgc gagcggcgcc ggacgggtgg cctggcaagc cggtccgcgc
     3841 gaaggcatac cgggcgtctg gtgagactgg ccgacgcagc cagggcgccc ggatggggcc
     3901 gcgcagtagg cgcagatggg tggattcagc gggcgtctgg tgagcgtggc cgacgcagcc
     3961 agggtgcccg gatggggctg cgcagtaggc gcagatcggt ggatccaggt ttagtcgtga
     4021 gtcgctcccc agcgcgtgac gtgatgggat ggcgcgatgg cgggggtgca gcgatacggc
     4081 ccggacgagc cgctcgcggt ggtgggtgga cggctgctga ccggtctgcg cgaggtcacc
     4141 gccgacctga ccgcgctcga ctccgccggg ttctgggcgg tggtgctgcc ctttgagggt
     4201 ccggcgatct gtgcccgatt cgagcgggtg cgaccggccc caccgtggcg tggacgtccg
     4261 tggctgggtc ccccgcgcga cgcctggcgc tcgtcgctgg acgaggcggg cttcgccgcc
     4321 ggagtgcgtc acattcgcga cgcgatcgcg gccggcggcg tctatcaggt caacctgacc
     4381 cggcggctca gcgcacccct gccgtcccgc acctcgatcg ccgcattggg tgccgccctg
     4441 gcgcaaggta acccggcccc gttcagtgcc gtcgtgcacc tgcccgcgca gggtgtgcac
     4501 gtggcgtccg cttcgccgga gttgtttctg agccgccgag gcgaggtggt gtggtcgtcg
     4561 ccgatcaagg gcacggcgaa gggcgtcgac gactttctgg ccaaggacgc ggccgagaac
     4621 gtgatgatcg tcgatctggt ccgcaacgat ctggggcggg tgtgccggcc gggctcggtc
     4681 gaggtgccca gcctgttggc cgttgagccg catcccgggc tgtttcacct ggtgtcgacg
     4741 gtgcggggcc ggctccgtgc gcgcatcggt tgggccgacc tgatcacggc cacgtttccg
     4801 cccggctcgg tcaccggcgc cccgaaactg gccgcactgg acgtgatcgc acaactcgag
     4861 ccggtggggc gcgggcccta ctgcggggcg atcggctggg tggacgccgg gcgcgtccgg
     4921 ggggagttga acgtggcgat ccgcacgttc tggatcgggg acgatcacct gcacctgggt
     4981 gtcggcggcg ggatcacctg ggattcgagt gcgtcggggg agtgggccga gaccgaactc
     5041 aaggcggcga acctgctgcg ggtcgcctcg cggtcggcgg tgcaccctcg cggggggtag
     5101 cccggcgcgc cccccagtgc tgcaatcatc gcatcgtaga atcggcgagc accaggcacg
     5161 tggacgcccg gcggcgagcg tcgaagaaga ggtgaatctg tgagtgacac ggtcaatcgc
     5221 cgcgggctca gcgtcggact gatgctgtgc gtgatggcga tctcgttcga gtcctacgcc
     5281 gtgctcaccg caatgcccgc cgccgcccgc gacctgggca acgtcggcct ctacgcctgg
     5341 accttcaccg cgttcgtcat cgcgatgacg ttcagcatcg tggcatccgg gcagttcacc
     5401 gaccggtacg gcccggtgca gccgatgatg gtcggtttcg gcgtgttcgc ggctgggctg
     5461 gtgatcgccg cactctccac gagcatgtgg gtactgctgg tggcccgctt cacccagggc
     5521 ctcggggcgg gcacgatgaa cgtcgctgtg atggtgctga tcgcccgcgc ctacgaagag
     5581 tccgaccgcg ccaaactgat gacctggttc tcggcctgct ggatgattcc ggctttcgtc
     5641 ggcccgccgc tggccggctg gatcgtgcag atcagctcat ggcacttcgt gttctggtcg
     5701 gtgctgccgc tgatggccgt cggcgggctg ctcgtgcttc gtccgctgcg tggcatcgaa
     5761 ctgacacccg aagagccgca cgaactgaag aagcggctga tgctgctggc cgccgccgtc
     5821 gccgcgggtc ttgcgctgat ccagtgggcc gggcagagcc tcaacctgtg gtcggccctg
     5881 tggttggtgg tgggcctggc cgccatcgtg ttcggcctgc cgccgttgat gcccaagggc
     5941 atcgactgga ccggcaaggg catctcgggt gtcgtgggtg cgcggttgtt cagcgccggt
     6001 gccttcttcg gcgcccagtc gttcctcacc ctgatgctga tcgaagagcg cggcctggac
     6061 gccgtgttca ccggtctgac gctgaccatt ggctcgctgg gttggatgag cggcgcgttg
     6121 ctgcaggcgc agagctggct gaaatggcgt cgcgaccaga tgattctggt cggtgtcgcg
     6181 gcgatcactg cgggcatcgc gctgctggcg gtcgccgcgt acattcccca gtcatggatc
     6241 gggctggtga tgatcgcctg gatcatcgcc ggcttcggca tgggcctgca gaaccccagc
     6301 acgtc
//
LOCUS       DAOSUG010000122         6302 bp    DNA     linear   ENV 10-JUN-2024
DEFINITION  MAG TPA_asm: Micropruina sp. isolate AMR_MDS_2195
            NODE_5813_length_6302_cov_5.010245, whole genome shotgun sequence.
ACCESSION   DAOSUG010000122 DAOSUG010000000
VERSION     DAOSUG010000122.1
DBLINK      BioProject: PRJNA925477
            BioSample: SAMN32864262
            Sequence Read Archive: SRR4236673
KEYWORDS    WGS; ENV; Metagenome Assembled Genome; MAG; Third Party Data; TPA;
            TPA:assembly.
SOURCE      Micropruina sp. (wastewater metagenome)
  ORGANISM  Micropruina sp.
            Bacteria; Bacillati; Actinomycetota; Actinomycetes;
            Propionibacteriales; Nocardioidaceae; Micropruina.
REFERENCE   1  (bases 1 to 6302)
  AUTHORS   Abdulkadir,N., Saraiva,J.P., Zhang,J., Stolte,S., Gillor,O.,
            Harms,H. and Rocha,U.
  TITLE     Genome-centric analyses of 165 metagenomes show that mobile genetic
            elements are crucial for the transmission of antimicrobial
            resistance genes to pathogens in activated sludge and wastewater
  JOURNAL   Microbiol Spectr 12 (3), e0291823 (2024)
   PUBMED   38289113
REFERENCE   2  (bases 1 to 6302)
  AUTHORS   Abdulkadir,N.
  TITLE     Direct Submission
  JOURNAL   Submitted (24-MAR-2023) Environmental Microbiology, Helmholtz
            Center for Environmental Research -UFZ, Permoserstr 15, Leipzig
            04318, Germany
COMMENT     The annotation was added by the NCBI Prokaryotic Genome Annotation
            Pipeline (PGAP). Information about PGAP can be found here:
            https://www.ncbi.nlm.nih.gov/genome/annotation_prok/
            
            ##Genome-Assembly-Data-START##
            Assembly Method        :: SPAdes v. 3.11.1
            Genome Representation  :: Full
            Expected Final Version :: Yes
            Genome Coverage        :: Not Applicable
            Sequencing Technology  :: Illumina MiSeq
            ##Genome-Assembly-Data-END##
            
            ##Genome-Annotation-Data-START##
            Annotation Provider               :: NCBI
            Annotation Date                   :: 05/04/2023 04:57:10
            Annotation Pipeline               :: NCBI Prokaryotic Genome
                                                 Annotation Pipeline (PGAP)
            Annotation Method                 :: Best-placed reference protein
                                                 set; GeneMarkS-2+
            Annotation Software revision      :: 6.5
            Features Annotated                :: Gene; CDS; rRNA; tRNA; ncRNA
            Genes (total)                     :: 2,776
            CDSs (total)                      :: 2,750
            Genes (coding)                    :: 2,714
            CDSs (with protein)               :: 2,714
            Genes (RNA)                       :: 26
            tRNAs                             :: 24
            ncRNAs                            :: 2
            Pseudo Genes (total)              :: 36
            CDSs (without protein)            :: 36
            Pseudo Genes (ambiguous residues) :: 0 of 36
            Pseudo Genes (frameshifted)       :: 2 of 36
            Pseudo Genes (incomplete)         :: 32 of 36
            Pseudo Genes (internal stop)      :: 2 of 36
            ##Genome-Annotation-Data-END##
FEATURES             Location/Qualifiers
     source          1..6302
                     /organism="Micropruina sp."
                     /mol_type="genomic DNA"
                     /submitter_seqid="NODE_5813_length_6302_cov_5.010245"
                     /isolate="AMR_MDS_2195"
                     /isolation_source="Activated sludge of municipal
                     wastewater treatment plants"
                     /db_xref="taxon:2737536"
                     /environmental_sample
                     /geo_loc_name="Canada"
                     /lat_lon="49.25 N 123.1 W"
                     /collection_date="2015-06-16"
                     /metagenome_source="wastewater metagenome"
                     /note="metagenomic"
     gene            <1..2765
                     /locus_tag="PLF56_05465"
     CDS             <1..2765
                     /locus_tag="PLF56_05465"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_013160152.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=3
                     /transl_table=11
                     /product="hypothetical protein"
                     /protein_id="HOQ53045.1"
                     /translation="LVANLDFVEGIFGNGGDPYLPENDASLDPGTWTGTTGCVILAPH
                     LIRLTKKELGLPHADAATERQKRDGMCWTSADERYNGGQAFKVCARDARGVIVTVIAD
                     NYFGYCKKEVKTQISYTANLFGNVEEEHAGGALVFPSYNIGGGYTDESATDDFRLEDV
                     LARDPQRFVRQPEGHALDTEHPEHVLVPARPTYSLRTMTVSWNTPSGERSIRLRADRT
                     YFGPNGYRVQLAQSPSDRTHWDLKAIVPTVTSCHKPCTVSGGGKSEISKAITDAFIFG
                     NAYVADFENDMDAVEAILARDHSDRFADPALRGSDTRPILSNSRSIGSVIKLLTPSDD
                     FTPEYNAWLQSIPPHVEELVFVVKRFHRPEWGSDWRSHFTVGIMNGRQGNALRLDGER
                     INVNMLRVGFDSDGSWRLFGLRHDFNPAVKVQTEDDITASIVGPAHLTERPGPVIGLS
                     RKYVQNCENLLFQRPDDAIVRGYDKQTERDMALPDNFLSNFEPLTRFDAREMRDDAVA
                     FSAFTAPMQQLLAGFADQPDDQSPRYVVSSANPRLVGGKPSKNPRYLQQRPDLTNASA
                     TAAADLAMHLQAKLPTHAPLPIPVDVVAAGRRNNPPEGPVPALCSFNPLHYMELPELF
                     MEYISSMTGKSPSTTGAGSEGALTKGPFNAMPAIIDLNAALLSYVLTGYDGWVSCAGY
                     VGPNVRVDHDISMLIPEVFSRMSDAERSATNLIAEGCLERVEDVRHDGQLVRASRLGY
                     RMTQKFARKYFGRIFLHPHVVFTPEMLRPELQDFGIYADSMRVIVETHRRVAQAYFDD
                     GTIELAIPPLRALLEIMAFGATADGHALDSDAVRALFDRDQVLTSDWYAARLDSKQQL
                     ATDRAQAVVDRLEAFAAHHKNAEACERLNVPNRIEAARAELDRFSSDDYRAALVGTLG
                     RQPLEG"
     gene            2890..3822
                     /locus_tag="PLF56_05470"
     CDS             2890..3822
                     /locus_tag="PLF56_05470"
                     /inference="COORDINATES: protein motif:HMM:NF024734.2"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="type IV toxin-antitoxin system AbiEi family
                     antitoxin domain-containing protein"
                     /protein_id="HOQ53046.1"
                     /translation="MKVRVDLPPDMQVLAERQAGVLSRDQLLRAGVSRRSVTRMLSTG
                     ALHPITPGFYRRWSEQTWLSRAWAGLLMGGSTAVLGGTTAGYLHKLVRDEPDTITVYT
                     TSQVVHRQGWQFIRGQRASRGEPARTRYEATVIDLCADRDEDGLAALLADALSSRRTT
                     TERLLTEVRRRTRVRHRKLLCEVLGDVSVGAHSALERRFLLNVERAHGLPCATRQAHA
                     RTKHRSDAWYEEFGLLVELDSKLHHSGGAAFHDMTRDNDHALAGLTTLRFGWGHVTGS
                     SACATARTLAAALSARGWAGPITSCPHCALVHPV"
     gene            3976..4353
                     /locus_tag="PLF56_05475"
     CDS             3976..4353
                     /locus_tag="PLF56_05475"
                     /inference="COORDINATES: ab initio
                     prediction:GeneMarkS-2+"
                     /note="Derived by automated computational analysis using
                     gene prediction method: GeneMarkS-2+."
                     /codon_start=1
                     /transl_table=11
                     /product="hypothetical protein"
                     /protein_id="HOQ53047.1"
                     /translation="MSYQLAFWAYADGRRSNRVADRRTYLKLIKGRRVRDVAPLDTDR
                     VLDELAIVYGTWRRTDTYHFSHPTHGAFDVWIAGGTFVVLTFHNVKDLTVMDPAIQCL
                     DAMGVPLYDPQVDRRFPWVARAI"
     gene            4383..4568
                     /locus_tag="PLF56_05480"
     CDS             4383..4568
                     /locus_tag="PLF56_05480"
                     /inference="COORDINATES: ab initio
                     prediction:GeneMarkS-2+"
                     /note="Derived by automated computational analysis using
                     gene prediction method: GeneMarkS-2+."
                     /codon_start=1
                     /transl_table=11
                     /product="hypothetical protein"
                     /protein_id="HOQ53048.1"
                     /translation="MTSKKVGDQPTTVLIAGDDQAEAARKLVLDRLSPEQLHSLTELL
                     GPAALALGPEGPFARPA"
     gene            4742..5146
                     /locus_tag="PLF56_05485"
     CDS             4742..5146
                     /locus_tag="PLF56_05485"
                     /inference="COORDINATES: protein motif:HMM:NF025442.2"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="DUF4258 domain-containing protein"
                     /protein_id="HOQ53049.1"
                     /translation="MRILNKLIVASLAALTMAFGLSLSAMASPVGESVTSSGALPQTV
                     PAGARCSVSYYTTHARLRMAERDISEWEVRNAIAYSCRHDKVVWQWLEGTYKYYGTYV
                     VVAMNPQGGVVTVWLAGGNGGGGWTQPTSRVI"
     gene            complement(5447..>6302)
                     /locus_tag="PLF56_05490"
     CDS             complement(5447..>6302)
                     /locus_tag="PLF56_05490"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_013160004.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=2
                     /transl_table=11
                     /product="glycerol-3-phosphate dehydrogenase"
                     /protein_id="HOQ53050.1"
                     /translation="AQLDEALDGADAVFIAVASVGVPKVTELALPAISKASVVLCTSK
                     GFWTDPDGRVQLLPDAIRTIAAENGALLPPIVGVGGPCKANEVAQGKPTATIYGSLSE
                     EAALRMAREISTEVYRIEPTTDEPGVEVCAPMKNVYAIALGIADGLEEGTGFPHHNLK
                     SAIFAQAVKEMSIASVALGARPETAYGLAGVGDLEVTGLSGRNKVYGVRIGRGENAIE
                     ALAAMEAAEQTVEGVPAAGLAMTLVQQQDGLLEQLPLLKAVTAILAGAEGPERLVADA
                     ALPKRVNF"
BASE COUNT         1107 a         2153 c         2023 g         1019 t
ORIGIN      
        1 gcctggtcgc caacctcgac ttcgtcgagg gcatcttcgg caacggcggc gacccttacc
       61 tgcccgagaa cgacgcgtcc ctcgacccgg gcacgtggac gggcaccacg ggttgcgtga
      121 tcctggcccc gcacctgatc aggctgacca agaaggaact agggctgccc cacgccgatg
      181 ccgccaccga acggcagaag cgtgacggca tgtgctggac gagcgccgac gagcgctaca
      241 acggcggcca ggcgttcaag gtctgtgccc gcgacgcccg cggtgtgatc gtcaccgtga
      301 tcgccgacaa ctacttcggc tactgcaaga aagaggtgaa aacccagatt agctacaccg
      361 cgaacctgtt cggcaacgtc gaagaagagc acgcgggtgg tgccctggtg ttcccctcct
      421 acaacatcgg cggcggctac accgacgaat ccgccaccga cgacttccgc ctcgaagacg
      481 tgctggcgcg cgatccgcag cggttcgtcc ggcagcccga ggggcacgcg ctcgacaccg
      541 aacaccccga gcatgtgctg gttccggccc gtcccaccta ttcgctgcgc accatgacgg
      601 tgtcgtggaa caccccctcg ggcgagcgat ccatccggct gcgcgccgat cgcacctact
      661 tcggccccaa cggctaccgg gtgcaactgg cgcagtcgcc gtccgatcgc acccactggg
      721 acctgaaggc gatcgtgccg accgtcacgt cctgtcacaa gccgtgcacg gtctcgggcg
      781 gcggcaagtc cgagatcagc aaggcgatca ccgacgcgtt catcttcggc aacgcctacg
      841 tggccgactt cgagaacgac atggacgccg tcgaggcgat cctggcccgc gaccattccg
      901 accggttcgc cgatccggcg ctgcgcggca gcgacacccg gccgatcctg tcgaacagcc
      961 gctcgatcgg cagcgtgatc aagctgctga ccccctccga cgacttcacc cccgagtaca
     1021 acgcatggct gcagagcatt ccgccgcacg tcgaagaact ggtgttcgtg gtgaaacggt
     1081 tccatcgccc cgagtggggt tcggattggc gcagccactt caccgtcggc atcatgaacg
     1141 gccggcaggg taacgcgctg cggctcgacg gtgagcggat caacgtcaac atgctgcggg
     1201 tcggcttcga ttcggacggt tcgtggcgtc tgttcgggct gcggcacgac ttcaaccccg
     1261 cggtgaaggt gcagaccgaa gacgacatca ccgcctcgat cgtcgggccg gcccacctca
     1321 cggagcgtcc gggcccggtg atcggactga gccgcaagta cgtccagaac tgcgagaacc
     1381 tgctgttcca gcgcccggac gacgcgatcg tgcgcggcta cgacaagcag accgagcgcg
     1441 acatggcgct gcccgacaac ttcttgtcga acttcgagcc gctgaccagg ttcgatgccc
     1501 gcgagatgcg cgacgacgcg gtcgcgttca gcgcgttcac ggcgccgatg cagcagttgc
     1561 tggccgggtt cgccgaccag cccgacgacc agagtccccg ctacgtggtg tcgtcggcga
     1621 acccgcggct ggtgggcggc aagccgtcca agaacccgcg ctacctgcag cagcgtcccg
     1681 acctgaccaa cgcctcggcg accgccgcgg ccgacctggc gatgcacctg caggccaagc
     1741 tgcccaccca tgcgccgctg ccgattccgg tcgacgtggt ggccgccgga cgccgcaaca
     1801 atccgccgga gggcccggtg ccggcactgt gctcgttcaa cccgctgcac tacatggagc
     1861 tgcccgagct gttcatggag tacatcagct cgatgaccgg caagtcgccc tcgaccaccg
     1921 gggccggttc ggagggtgcc ctgaccaagg gcccgttcaa cgcgatgccg gcgatcatcg
     1981 acctgaacgc ggccctgctg agctacgtgc tcaccgggta cgacggctgg gtgtcgtgcg
     2041 ccggctacgt cggccccaac gtgcgggtcg atcacgacat ctcgatgttg attcccgagg
     2101 tgttctcgcg catgagcgat gccgagcgct cggccacgaa cctgatcgcc gaagggtgcc
     2161 tggagcgggt cgaggacgtc aggcatgacg gtcagctggt ccgggccagc cggttgggct
     2221 accgcatgac gcagaagttc gcccgcaagt acttcgggcg catctttctg catccgcacg
     2281 tggtgttcac ccccgagatg ctgcggccgg aactgcagga cttcggcatc tacgccgaca
     2341 gcatgcgcgt gatagtcgaa acccaccgcc gggtcgccca ggcctacttc gacgacggca
     2401 ccatcgagtt ggcgattccg ccgctgcgcg ccctgctcga aatcatggcg ttcggcgcca
     2461 cagccgatgg ccacgcgctc gactcggacg cggtgcgggc cctgttcgac cgcgaccagg
     2521 tgctgacctc ggactggtat gcggcccggc tcgattccaa gcagcaactg gccaccgacc
     2581 gggcgcaagc cgtggtcgat cggctcgagg ccttcgcagc acaccacaag aacgccgagg
     2641 cctgcgagcg gctgaacgtg ccgaaccgca tcgaggccgc gcgggccgaa ctcgaccggt
     2701 tcagcagcga cgactaccgg gcggccctgg tcggcaccct gggtcgccag ccgttggagg
     2761 gctgaaggac tggactggca aagacagtgt gggatcgagc accgagtatg gtgctcgatc
     2821 ccgcactgtc ctgcgggcgg gcctcagccg atgtgaacac ttcggccgcg ccgtgcccac
     2881 actgaggtcg tgaaggttcg agtcgacttg ccacccgaca tgcaggtgct cgccgaacgc
     2941 caggccgggg tgctttcgcg cgaccaactg ctgcgcgcgg gagtgtcgcg ccggtccgtc
     3001 acacgcatgc tgtcaaccgg tgctctgcac ccgatcaccc ccggcttcta tcggcggtgg
     3061 agcgagcaga cctggctgag ccgagcctgg gccggcctgc tgatgggggg atcgacggcg
     3121 gtgctggggg gtacaacggc cggatatctg cacaagctcg tgcgcgacga gcccgacacg
     3181 atcacggtgt acaccaccag ccaggtggtg caccggcagg ggtggcagtt catccgcggg
     3241 cagcgcgctt cgcgaggtga accggcgcgc acgcgctacg aggccacggt gatcgacttg
     3301 tgcgccgatc gcgacgagga cggcctggct gcgctgctcg ccgatgcgct gagcagtcgg
     3361 cgcaccacaa ccgaacgact tctgaccgaa gtgcgccgac gcactcgcgt gcgccaccgg
     3421 aaactgctgt gcgaggtact gggcgacgtg tcagtcggag cacattcggc gcttgagcga
     3481 cgcttcctgc tcaacgtgga acgcgcgcac ggcctgccct gcgcgactcg tcaggcgcac
     3541 gcccgcacga agcatcgcag tgacgcctgg tacgaagagt tcggactgct ggtggagctc
     3601 gacagcaagt tgcaccacag cggtggcgca gccttccacg acatgacgcg tgacaatgac
     3661 catgctctcg ccgggctgac caccttgcga ttcggctggg ggcacgtcac cggttcgtcg
     3721 gcctgcgcga ccgcgagaac gctggcggcg gcactgtcgg cgcgcgggtg ggcgggtcct
     3781 atcacgtcat gcccgcactg cgcgctggtt cacccggtct agctcatggg atcaaaacac
     3841 agtgtgaaat cgagaactga gtatggtgct cgatcccgca ctgtcttccg ggcatcgccg
     3901 cgccaacccc aaagatcgcc tgagacgccg cgtcgcgcac cgccccaccg ccgcagggca
     3961 actacatttc ggcctgtgag ctatcaactg gcgttctggg cctatgccga cggacggcgc
     4021 agcaaccggg tggccgaccg gcgcacctac ctcaagttga tcaagggccg ccgggtcaga
     4081 gacgtcgcgc cgctcgacac cgaccgggtg ctggacgaac tcgccatcgt ctatggcacc
     4141 tggcgccgca ccgacaccta ccacttcagc caccccacgc acggcgcctt cgacgtgtgg
     4201 atcgcgggcg gcaccttcgt ggtgctcacc ttccacaacg tgaaggacct gaccgtgatg
     4261 gaccccgcga tccagtgcct ggacgccatg ggcgtgccgc tgtacgaccc tcaggtggac
     4321 cgccgcttcc cctgggtggc ccgcgccatc tgaacgggcc gtcgccaatc ttcgccgcca
     4381 ccttgaccag caagaaggtc ggcgaccagc ccaccacggt gctgatcgcc ggcgacgacc
     4441 aggccgaggc tgcccgcaag ctggtcctgg accggttgag tcccgaacag ctccacagcc
     4501 tgaccgaact gctcggtccg gcggcgctcg ccctcgggcc cgagggaccg ttcgcacgtc
     4561 ccgcgtgagg gcgccggggc ggccgatgaa gacccttcca ctcggctccg atcgcgcgag
     4621 ccaaccgagt gcccttggcc gaaacgatct atcatgacga agtcgagaag gcgtgagtgg
     4681 tgcggcccgg ctacgggggc atagccattg acttcgcggt gaaccgagtt cagaatatgt
     4741 catgcgaatt ctcaacaagt tgatcgttgc aagtcttgcg gccttgacca tggcattcgg
     4801 gttatcgctc tcagcgatgg ccagtcccgt tggtgaatct gtcacttctt cgggagcact
     4861 gcctcaaact gtacctgcag gtgctcgctg cagcgtctcg tactacacca cccacgcccg
     4921 gctgcgcatg gcggaaaggg acatttcgga gtgggaggtg agaaatgcaa tcgcttactc
     4981 ttgtaggcac gacaaggtgg tgtggcagtg gttagaaggc acatacaagt actacggcac
     5041 ctacgtcgtc gtagccatga atcctcaggg cggggtggtc acagtttggc ttgcaggcgg
     5101 aaatggtgga ggcggttgga cgcagccaac ttcacgggtg atctagatgc acttcctgac
     5161 tcgcagtgta caaatccagt tcgatgagac agcgaatgcc gccgtagtga agttcgggcc
     5221 ggattttcct caacggtctg aagtcgtcga cactccaatc gagattgacg gaaacgtagt
     5281 agccatcctc acgagtaata gtgaaggact gatcgtccag ttggagttgc tgagagcaga
     5341 tcgccaactt gggcctatag tcgagtagct acacgattga agaccgcgtc tgttgggtga
     5401 gaggaccgag acggaacacg ccactctcat cacgcgaatt ggacagctag aagttcaccc
     5461 gcttcggcag cgccgcgtcg gcgaccagcc gctccgggcc ctccgcgccg gcgagaatcg
     5521 ccgtcaccgc cttgagcagc ggaagctgct ccagcaggcc gtcctgctgc tgcaccaggg
     5581 tcatcgccag gcccgccgcg ggcacgccct cgacggtctg ctcggccgcc tccatcgcgg
     5641 ccaacgcctc gatggcgttc tcaccgcggc cgatccgcac cccgtaaacc ttgttgcggc
     5701 ccgacagccc ggtgacctcg aggtcgccga cgccggccag tccgtacgcg gtctcaggac
     5761 gggccccgag cgccaccgac gcgatcgaca tctctttgac cgcctgggcg aagatcgccg
     5821 acttcaggtt gtggtgcgga aagccggtgc cctcctccag cccgtcggcg atgccgagcg
     5881 cgatcgcgta cacgttcttc atgggggcgc acacctcgac gcccggctcg tcggtggtcg
     5941 gctcgatgcg atacacctca gtgctgatct cgcgcgccat gcgcagcgcc gcctcttcgc
     6001 tcagcgagcc gtagatggtg gcggtcggct tgccctgcgc gacctcgttg gccttgcacg
     6061 gcccaccgac gccgacgatc ggcggcagca gcgcgccgtt ctcggccgcg atggtgcgga
     6121 tcgcgtccgg cagcagttgc acccggccgt ccgggtcggt ccagaagccc ttcgacgtgc
     6181 acaacaccac cgaggccttc gagatggcgg gcagggccag ctcggtcacc ttcggcacac
     6241 cgaccgaggc caccgcgatg aacacggcgt cggcgccgtc caatgcctcg tccagttgag
     6301 cg
//
LOCUS       DAOSUG010000123         6265 bp    DNA     linear   ENV 10-JUN-2024
DEFINITION  MAG TPA_asm: Micropruina sp. isolate AMR_MDS_2195
            NODE_5852_length_6265_cov_4.866989, whole genome shotgun sequence.
ACCESSION   DAOSUG010000123 DAOSUG010000000
VERSION     DAOSUG010000123.1
DBLINK      BioProject: PRJNA925477
            BioSample: SAMN32864262
            Sequence Read Archive: SRR4236673
KEYWORDS    WGS; ENV; Metagenome Assembled Genome; MAG; Third Party Data; TPA;
            TPA:assembly.
SOURCE      Micropruina sp. (wastewater metagenome)
  ORGANISM  Micropruina sp.
            Bacteria; Bacillati; Actinomycetota; Actinomycetes;
            Propionibacteriales; Nocardioidaceae; Micropruina.
REFERENCE   1  (bases 1 to 6265)
  AUTHORS   Abdulkadir,N., Saraiva,J.P., Zhang,J., Stolte,S., Gillor,O.,
            Harms,H. and Rocha,U.
  TITLE     Genome-centric analyses of 165 metagenomes show that mobile genetic
            elements are crucial for the transmission of antimicrobial
            resistance genes to pathogens in activated sludge and wastewater
  JOURNAL   Microbiol Spectr 12 (3), e0291823 (2024)
   PUBMED   38289113
REFERENCE   2  (bases 1 to 6265)
  AUTHORS   Abdulkadir,N.
  TITLE     Direct Submission
  JOURNAL   Submitted (24-MAR-2023) Environmental Microbiology, Helmholtz
            Center for Environmental Research -UFZ, Permoserstr 15, Leipzig
            04318, Germany
COMMENT     The annotation was added by the NCBI Prokaryotic Genome Annotation
            Pipeline (PGAP). Information about PGAP can be found here:
            https://www.ncbi.nlm.nih.gov/genome/annotation_prok/
            
            ##Genome-Assembly-Data-START##
            Assembly Method        :: SPAdes v. 3.11.1
            Genome Representation  :: Full
            Expected Final Version :: Yes
            Genome Coverage        :: Not Applicable
            Sequencing Technology  :: Illumina MiSeq
            ##Genome-Assembly-Data-END##
            
            ##Genome-Annotation-Data-START##
            Annotation Provider               :: NCBI
            Annotation Date                   :: 05/04/2023 04:57:10
            Annotation Pipeline               :: NCBI Prokaryotic Genome
                                                 Annotation Pipeline (PGAP)
            Annotation Method                 :: Best-placed reference protein
                                                 set; GeneMarkS-2+
            Annotation Software revision      :: 6.5
            Features Annotated                :: Gene; CDS; rRNA; tRNA; ncRNA
            Genes (total)                     :: 2,776
            CDSs (total)                      :: 2,750
            Genes (coding)                    :: 2,714
            CDSs (with protein)               :: 2,714
            Genes (RNA)                       :: 26
            tRNAs                             :: 24
            ncRNAs                            :: 2
            Pseudo Genes (total)              :: 36
            CDSs (without protein)            :: 36
            Pseudo Genes (ambiguous residues) :: 0 of 36
            Pseudo Genes (frameshifted)       :: 2 of 36
            Pseudo Genes (incomplete)         :: 32 of 36
            Pseudo Genes (internal stop)      :: 2 of 36
            ##Genome-Annotation-Data-END##
FEATURES             Location/Qualifiers
     source          1..6265
                     /organism="Micropruina sp."
                     /mol_type="genomic DNA"
                     /submitter_seqid="NODE_5852_length_6265_cov_4.866989"
                     /isolate="AMR_MDS_2195"
                     /isolation_source="Activated sludge of municipal
                     wastewater treatment plants"
                     /db_xref="taxon:2737536"
                     /environmental_sample
                     /geo_loc_name="Canada"
                     /lat_lon="49.25 N 123.1 W"
                     /collection_date="2015-06-16"
                     /metagenome_source="wastewater metagenome"
                     /note="metagenomic"
     gene            <1..587
                     /locus_tag="PLF56_05495"
     CDS             <1..587
                     /locus_tag="PLF56_05495"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_017934049.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_function: GO:0008270 - zinc ion binding [Evidence IEA]"
                     /codon_start=3
                     /transl_table=11
                     /product="zinc finger domain-containing protein"
                     /protein_id="HOQ53051.1"
                     /translation="EGVSSSDSQPGSHHAVHQLRFVDQRMFGGLSLSVGGAELPRELA
                     HIGRDPFDPDFDLAAAVRTVRSKRSGIKRVLLDQTVVSGIGNIYADEALWRARLHYDR
                     AANTLSAKQARDLLTQAQDVMAQALAQGGTSFDSLYVNVNGSSGYFERALAAYGREGL
                     PCLRCGRPIVREAFMNRSSYRCPTCQRRPRRSAR"
     gene            598..1155
                     /locus_tag="PLF56_05500"
     CDS             598..1155
                     /locus_tag="PLF56_05500"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_013161310.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_component: GO:0016020 - membrane [Evidence IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="GtrA family protein"
                     /protein_id="HOQ53052.1"
                     /translation="MSSAGSLYRRYAHVGRQFIKFGLVGGAGVVVNMLVAIVMNKLNG
                     GTINAQNVLFAIPGTPFNFRFTTLVWIVAFMVANLFNFQLNRHWTFKGKRAPWLTEFL
                     PFLAVGSAAALVGIFIKLGFTNPTSPFYLPEPWFHENAGLRSREYWAQLFTILITMPI
                     NFLVNKLWTFRHVRNAHTDSLAGTR"
     gene            1160..4717
                     /gene="smc"
                     /locus_tag="PLF56_05505"
     CDS             1160..4717
                     /gene="smc"
                     /locus_tag="PLF56_05505"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_016670135.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_function: GO:0005524 - ATP binding [Evidence IEA];
                     GO_process: GO:0007059 - chromosome segregation [Evidence
                     IEA];
                     GO_process: GO:0030261 - chromosome condensation [Evidence
                     IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="chromosome segregation protein SMC"
                     /protein_id="HOQ53053.1"
                     /translation="MHLKSLTLRGFKSFASTTSLAFEPGITCVVGPNGSGKSNVVDAL
                     AWVMGEQGAKSLRGGKMEDVIFAGTSARAPLGRAEVTLTIDNTDGALPIDYTEVTISR
                     TMFRSGGSEYKINGSTARLLDVQDLLSDSGIGREMHVIVGQGQLDSILQATPEIRRGF
                     IEEAAGVLKHRKRKEKALRKLEATSVNLNRLSDLLAELRRQLKPLGRQAEVARKAAVI
                     QIELRDAKARLMADDYVQASLALESELAAEADLKARRLVVEAALDAARAAEAEAETAS
                     RESLPRVNAAQDTWYGLAGLRERVSTTISIAGERVRNANAAGEGDRPGRDPELIEREA
                     AEMRKAEAALAIEVAAAEKGLTQATEALRASEQAHEQAERTFTAQQRAIADRREGLAR
                     LTGEVNTLRSRTEAAGSEIDRLTSARDDALNRAAEAKHRFAALETRLSGVSEGESGLD
                     DAYERAAEQVASIEAAITESQRGEQKAQTERASLAARLDGLALGLERKDASAALLAAG
                     ESIGGLLGSVAALVAVQPGYETAVSAALGSAADAVAVAGLDAAVDAFGHLKAADLGRA
                     GLLLGGSPEPGNDTWPELPSEAVWAIDVVTCGAQVRPALVRLLRKVALVPDLPAARTL
                     VAGLPDVTAVTRDGDVLSQWFASGGSSAKPSLIEVQAAIDETQGRLNAAQAEVERARF
                     ALAQHQAELAQAREAAEFALARLHESDAQHAALAEELAALSQTTRSAQAEATRLEASI
                     GAAQATREKNREALSALEQRLELASAQTGVVEADPGERDRLGEVARTARGAEMEARLA
                     LRTIEERARALAGRADSLQRAAQAERQARQRAAARREQLRREAEDARAVGVAAAWLLA
                     RVEASHELAAAERSAAEAARAQAEATLAEARRSGRELAKQFEELVDSVHRDELARAEQ
                     RVRLEALAEKALLELGLDADVLVAEYGPDQLVPVLTGADGAALAPDDEKPEPVAFVRA
                     EQEKRLRAAERNLGVLGKVNPLALEEFDALQERHAFLAEQLDDLRKTRADLADIIAEV
                     DTRVQEVFAEAYADVEVAFEQSFARLFPGGEGRLVLTEPGAWLTTGVDVEARPAGKKV
                     KRLSLLSGGERSLVAVAFLVALFKARPSPFYILDEVEAALDDVNLGRLLQIYEELREN
                     SQLLVITHQKRTMEIADALYGVTMRADGVSAVISQRLRD"
     gene            5014..5316
                     /locus_tag="PLF56_05510"
     CDS             5014..5316
                     /locus_tag="PLF56_05510"
                     /inference="COORDINATES: ab initio
                     prediction:GeneMarkS-2+"
                     /note="Derived by automated computational analysis using
                     gene prediction method: GeneMarkS-2+."
                     /codon_start=1
                     /transl_table=11
                     /product="hypothetical protein"
                     /protein_id="HOQ53054.1"
                     /translation="MIHPPAVRHTGRIPSGRLIVAVALLVMVVILAVAIGIVAIVAMG
                     MQGAGRDKVPELANVMAETARHLNGDAEPPGRLVEFFEEIPDVRRGSSAKSANSPK"
     gene            5381..>6265
                     /locus_tag="PLF56_05515"
     CDS             5381..>6265
                     /locus_tag="PLF56_05515"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_004808599.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_function: GO:0005525 - GTP binding [Evidence IEA];
                     GO_process: GO:0006614 - SRP-dependent cotranslational
                     protein targeting to membrane [Evidence IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="signal recognition particle receptor subunit
                     alpha"
                     /protein_id="HOQ53055.1"
                     /translation="MDLLQLLIIGILGLAVVVAGTVFLARRQAVHGRAKPELPAKPSA
                     EPQPPAVQQGDQPGEPTGDAPGAAAATTLERPEAPANRMARLRRKLAGSTNPLARGLL
                     SILSSDRLDADTWDDFEATLITSDLGVGPTQELTEALRRELAIDGVNDPARAKQTLRT
                     ELLKLVNPHLDRTLKVEGSDGKPGVVLVVGVNGTGKTTTIGKISRVLVAEDKSVVLGA
                     ADTFRAAAADQLQTWGDRVGVPTVRGPEGGDPAAVAYQAVQRGIDDGADVVVVDTAGR
                     LHTKTGLMDELGKIKRVIE"
BASE COUNT          977 a         2167 c         2222 g          899 t
ORIGIN      
        1 ctgagggcgt ctcgtcgtcc gactcacagc cgggatctca tcacgccgta caccaactcc
       61 gcttcgtcga ccaacgcatg tttggcgggt tgtcgctgag cgtgggcggt gccgagttgc
      121 cgcgcgaact cgcccacatc ggccgggacc cgttcgatcc tgacttcgac ctcgccgccg
      181 ccgtccgtac cgtgcgcagc aaacggtcgg gcatcaagcg cgtgctgctc gaccagaccg
      241 tggtgtcggg catcggcaac atctatgccg acgaggccct gtggcgggcc cgcctgcact
      301 acgaccgcgc cgccaacacg ctgagcgcca agcaggcgcg cgacctgctc acccaggccc
      361 aggacgtgat ggcgcaggcc ctcgctcagg gcggcacctc gttcgactcg ctgtacgtca
      421 acgtgaacgg atcgtcgggc tacttcgagc gtgccctcgc cgcgtacgga cgggagggtc
      481 tgccgtgtct gcgctgcgga cgtccgatcg tgcgcgaggc gttcatgaac cgctcgtcgt
      541 atcgctgccc gacgtgccaa cgccgtccgc gtcgctcggc ccgatagggt gcccctcgtg
      601 agttctgcag ggtcgttgta tcgccgctac gcgcacgtgg gtcggcagtt catcaagttc
      661 ggcctcgtcg gcggcgccgg cgtggtggtc aacatgctgg tcgccatcgt gatgaacaag
      721 ctcaacggcg gcaccatcaa cgcccagaac gtgctgttcg cgattcccgg cacgccgttc
      781 aactttcgct tcacgaccct ggtctggatc gtcgccttca tggtggcgaa cctgttcaac
      841 tttcagctca accggcactg gaccttcaaa ggcaagcgcg ccccctggtt gacggagttt
      901 ctgccgtttc tggcggtggg cagcgccgcc gcactggtcg gcatcttcat caagctcggc
      961 ttcacgaacc cgacctcgcc gttctacctg cccgagccct ggttccatga gaacgccggc
     1021 ctgcgctcgc gtgagtactg ggcccagctg ttcaccattc tgatcaccat gccgatcaac
     1081 tttctggtca acaagctgtg gaccttccgc cacgtccgca acgcgcacac cgactcgctg
     1141 gccggcaccc ggtaggccca tgcatctgaa gtccctgacc ctgcgcgggt tcaagtcgtt
     1201 cgcctcgacc acgtccctgg cgttcgagcc gggtatcacc tgcgtggtgg gccccaacgg
     1261 gtccggcaag tccaacgtgg tggacgccct ggcctgggtg atgggcgaac agggcgccaa
     1321 gtcgttgcgt ggcggcaaga tggaggacgt catcttcgcc ggcacgtccg cccgggcacc
     1381 gctgggccgc gccgaggtga cgctgaccat cgacaacacc gacggcgccc tgccgatcga
     1441 ttacaccgag gtgacgatca gccgcacgat gttccgttcc ggcggttcgg agtacaagat
     1501 caacggcagc acggcgcggc tgctcgacgt gcaggatctg ctgtccgatt caggcatcgg
     1561 ccgagagatg cacgtgatcg tcgggcaggg ccagctcgac tcgatccttc aggcaacgcc
     1621 cgagattcgc cgcggcttca tcgaagaggc cgccggggtg ctgaagcacc gcaagcgcaa
     1681 agagaaggcg ctgcgcaagc tcgaagccac cagcgtcaac ctcaaccggc tcagcgacct
     1741 gctggccgaa ctgcgccgcc agctcaagcc gctgggacgt caggccgagg tggcccgcaa
     1801 agcggccgtc attcagatcg agttgcgcga tgcgaaggcc cggctgatgg cggacgacta
     1861 cgtccaggcc agccttgcgc tcgaatcaga actggccgcc gaggccgacc tcaaggcgcg
     1921 ccggctcgtg gtcgaggcag ccctggacgc ggcccgcgcg gccgaggccg aagccgaaac
     1981 ggccagccgc gagtccctgc cccgggtcaa cgcggcgcag gacacctggt acggcctggc
     2041 cgggctgcgc gaacgggtct cgaccacgat cagcatcgcc ggtgagcggg tgcgcaacgc
     2101 caacgccgcc ggcgaggggg atcgtccggg ccgcgacccc gaactgatcg agcgcgaagc
     2161 cgccgagatg cgcaaggccg aagcggcgct ggcgatcgag gtggcggctg ccgaaaaggg
     2221 gttgacgcag gccaccgagg cacttcgggc gtccgagcag gcccatgagc aggccgaacg
     2281 caccttcacc gcccaacagc gcgccatcgc ggatcgtcgc gaagggctgg cccggctgac
     2341 cggcgaggtg aacacgctgc gcagccgcac cgaggcggca gggtccgaga tcgaccggct
     2401 gacctctgcg cgtgacgacg cgctgaatcg ggcggccgag gccaagcacc gcttcgccgc
     2461 cctcgagacc cggctgtcgg gggtcagcga gggcgaatcg ggcctggacg atgcctacga
     2521 gcgggccgcc gagcaggtgg cgagcatcga ggctgcgatc accgagtcgc agcgcggcga
     2581 gcagaaggca cagaccgagc gggcctcgct cgccgcccgg ctcgacggcc tggcgctggg
     2641 gctcgaacgc aaggacgcct cggcggcact gctcgcggcc ggtgaatcga tcggcggatt
     2701 gctggggtcg gtggcagccc tggtcgcggt acaacccggc tacgagaccg cggtgtcggc
     2761 cgcgctcggc tcggccgcgg acgccgtcgc ggtcgcgggt ctcgatgcgg cggtcgacgc
     2821 cttcgggcac ctcaaggccg cagatcttgg acgggccgga ctgctgctcg gcgggtcgcc
     2881 cgagccgggc aacgacacct ggcccgagct gccgtccgag gcggtgtggg cgatcgacgt
     2941 ggtgacctgc ggggcacagg tccggccggc gctggtgcgg ctgctgcgca aggtcgccct
     3001 ggtgcccgac cttcccgcgg cacggacgct ggtggcgggg ctgcccgacg tgaccgcggt
     3061 cacccgcgac ggcgacgtgc tcagccagtg gttcgcctcc ggcggatcgt ccgccaaacc
     3121 gtcgctgatc gaggtacagg cggcgatcga cgagacgcag gggcggctca acgcggccca
     3181 ggccgaggtc gagcgtgcgc ggttcgcgct ggcccagcac caggccgagc tggcgcaggc
     3241 acgcgaggcc gctgagttcg cgctcgcccg gctgcacgaa tccgacgccc agcatgccgc
     3301 cctggccgaa gagctggccg cgctcagcca gaccacccgc tcggcgcagg cggaggcgac
     3361 ccggctggag gcgtccatcg gtgcggcgca ggccacgcgg gaaaagaacc gcgaggcgct
     3421 gtccgccctg gagcaacgcc tcgaactggc gtccgcgcag accggggtgg tcgaggccga
     3481 tccgggtgag cgcgatcgcc tgggcgaggt ggcgaggacg gcccgcggcg ccgagatgga
     3541 ggcccggctg gccctgcgca ccattgagga gcgggcccgc gccctggctg gccgggccga
     3601 ctcgctgcaa cgcgcggccc aggccgagcg ccaggcccgg cagcgcgcgg cggcacggcg
     3661 cgagcaactg cgtcgcgagg ccgaggacgc gcgcgcggtc ggtgtggccg cggcctggct
     3721 gttggcccgg gtggaggcgt cccacgagct ggccgccgcc gagcgcagcg ccgccgaagc
     3781 ggcccgtgcg caggccgaag cgaccctggc tgaggcccgt cgcagcggac gtgagctggc
     3841 caaacagttc gaagagcttg tcgattcggt ccaccgcgac gagttggccc gggccgagca
     3901 gcgagtccgg ctggaggcgc ttgccgagaa ggcgctgctc gaactcggcc tcgacgccga
     3961 cgtgctggtc gccgaatacg gaccggacca gctggttccg gtgctgaccg gtgccgacgg
     4021 cgcggcgctc gcgcccgatg acgagaaacc cgagcccgtc gcgttcgtcc gcgcggagca
     4081 ggagaagcgg ttgcgggcgg ccgaacgcaa cctgggtgtg ctcggcaagg tcaacccgct
     4141 ggcgctggaa gagttcgacg ccctgcagga gcggcacgcg tttctggccg agcagctgga
     4201 cgacctgcgc aagacccggg ccgacctggc cgacatcatc gccgaggtcg acacccgggt
     4261 gcaggaggtc ttcgcggagg cgtatgccga tgtcgaggtg gctttcgaac agtcgttcgc
     4321 gcggctgttc cccgggggcg agggacggct ggtgctcacc gagcccggcg cctggttgac
     4381 caccggcgtg gacgtcgaag cccgtccggc cggcaagaag gtgaagcggc tctcgctgct
     4441 gtcgggcggt gagcgctcat tggtcgcggt ggcgtttctg gtcgcgctgt tcaaggcacg
     4501 accgagtccg ttctacattc tcgacgaggt cgaggcggcg ctggacgacg tcaacctggg
     4561 ccggctgctg cagatctatg aggaactgcg cgagaacagc cagctgctgg tgatcaccca
     4621 ccagaagcgc acgatggaga tcgcggacgc cctctacggc gtcaccatgc gcgccgacgg
     4681 cgtctcggcg gtgatcagcc agcggctgcg cgactgagca cgatggggga ctgttcccgc
     4741 ttcgtgctcc ggccggttgc actctgagtg cggcgacccg cgcagtcgtg ccccaccacc
     4801 atcctcgcca gaagccgcgt cctgttgctg atcaggacac atgcaggccc gcttcgcgcg
     4861 ccggacagtc gcgtcgattc tgaatcgacc cgcaggggtc gtcacgaaga ccggcgtgtc
     4921 gcgggtcggg tgctcgactg gtggccggga tctcagaggg tcgacgcgac acccggatct
     4981 cgatccgaac agcagaccac gccctcgcgc cgcatgatcc atccgccggc cgtgcggcat
     5041 actggacgca tcccgtccgg aaggctgatc gtggctgtcg cactgctcgt catggtggtg
     5101 atcctcgccg ttgccatcgg catcgtcgcc atcgtcgcca tgggtatgca gggggccggg
     5161 cgtgacaagg tgcccgagtt ggccaacgtg atggccgaaa ccgcgcggca cctcaacggc
     5221 gacgccgaac ctccgggccg tctggtcgag ttcttcgaag agattcccga cgtgcggcgc
     5281 gggtcgtccg caaagtcggc caactcgccc aagtagcgcc cgccgtccgc accccgtatg
     5341 gctgcaatgc gcgcggctcg ctcacccgta ggctgaccgg gtggaccttc ttcagctgct
     5401 gatcatcggc attctcggct tggccgtcgt ggtggcgggc accgtctttc tggcgcgccg
     5461 ccaggcagtg cacgggcgcg ccaagcccga actgcccgcg aagccgtcgg ctgaaccgca
     5521 gccgcccgcc gtccagcagg gcgaccaacc tggcgagccg accggcgacg cgccaggggc
     5581 ggccgcggcc accaccctgg agcgtcctga ggcgccggcg aaccgcatgg cgagactgcg
     5641 ccgcaagctg gcgggcagca ccaatccgtt ggcgcgcggg ttgctgtcca tcctgtcgtc
     5701 cgaccggctg gacgccgaca cctgggacga cttcgaggcc acgctgatca cctccgacct
     5761 cggggtcggg ccgacccagg agctcaccga ggcgctacga cgcgagttgg cgatcgacgg
     5821 ggtcaacgac ccggccaggg ccaaacagac tctgcgcacc gagctgctga agctggtcaa
     5881 tccccatctc gaccgcacgc tcaaggtcga aggctcggac ggcaagcccg gcgtggtgct
     5941 cgtggtgggt gtcaacggca ccggcaagac caccacgatc ggcaagatct ctcgcgtgct
     6001 ggtcgccgag gacaagtcgg tggtgctggg cgccgcagac accttccgtg cggcggccgc
     6061 cgaccaactg cagacctggg gcgaccgggt cggcgtgccg accgtacgcg ggcccgaggg
     6121 tggcgacccg gccgcggtgg cctaccaggc ggtccagcgc ggcatcgacg acggtgcgga
     6181 cgtggtcgtg gtcgacaccg ccggccggct gcacaccaag accggcctga tggacgagct
     6241 cggcaagatc aagcgggtga tcgag
//
LOCUS       DAOSUG010000124         6262 bp    DNA     linear   ENV 10-JUN-2024
DEFINITION  MAG TPA_asm: Micropruina sp. isolate AMR_MDS_2195
            NODE_5858_length_6262_cov_4.537780, whole genome shotgun sequence.
ACCESSION   DAOSUG010000124 DAOSUG010000000
VERSION     DAOSUG010000124.1
DBLINK      BioProject: PRJNA925477
            BioSample: SAMN32864262
            Sequence Read Archive: SRR4236673
KEYWORDS    WGS; ENV; Metagenome Assembled Genome; MAG; Third Party Data; TPA;
            TPA:assembly.
SOURCE      Micropruina sp. (wastewater metagenome)
  ORGANISM  Micropruina sp.
            Bacteria; Bacillati; Actinomycetota; Actinomycetes;
            Propionibacteriales; Nocardioidaceae; Micropruina.
REFERENCE   1  (bases 1 to 6262)
  AUTHORS   Abdulkadir,N., Saraiva,J.P., Zhang,J., Stolte,S., Gillor,O.,
            Harms,H. and Rocha,U.
  TITLE     Genome-centric analyses of 165 metagenomes show that mobile genetic
            elements are crucial for the transmission of antimicrobial
            resistance genes to pathogens in activated sludge and wastewater
  JOURNAL   Microbiol Spectr 12 (3), e0291823 (2024)
   PUBMED   38289113
REFERENCE   2  (bases 1 to 6262)
  AUTHORS   Abdulkadir,N.
  TITLE     Direct Submission
  JOURNAL   Submitted (24-MAR-2023) Environmental Microbiology, Helmholtz
            Center for Environmental Research -UFZ, Permoserstr 15, Leipzig
            04318, Germany
COMMENT     The annotation was added by the NCBI Prokaryotic Genome Annotation
            Pipeline (PGAP). Information about PGAP can be found here:
            https://www.ncbi.nlm.nih.gov/genome/annotation_prok/
            
            ##Genome-Assembly-Data-START##
            Assembly Method        :: SPAdes v. 3.11.1
            Genome Representation  :: Full
            Expected Final Version :: Yes
            Genome Coverage        :: Not Applicable
            Sequencing Technology  :: Illumina MiSeq
            ##Genome-Assembly-Data-END##
            
            ##Genome-Annotation-Data-START##
            Annotation Provider               :: NCBI
            Annotation Date                   :: 05/04/2023 04:57:10
            Annotation Pipeline               :: NCBI Prokaryotic Genome
                                                 Annotation Pipeline (PGAP)
            Annotation Method                 :: Best-placed reference protein
                                                 set; GeneMarkS-2+
            Annotation Software revision      :: 6.5
            Features Annotated                :: Gene; CDS; rRNA; tRNA; ncRNA
            Genes (total)                     :: 2,776
            CDSs (total)                      :: 2,750
            Genes (coding)                    :: 2,714
            CDSs (with protein)               :: 2,714
            Genes (RNA)                       :: 26
            tRNAs                             :: 24
            ncRNAs                            :: 2
            Pseudo Genes (total)              :: 36
            CDSs (without protein)            :: 36
            Pseudo Genes (ambiguous residues) :: 0 of 36
            Pseudo Genes (frameshifted)       :: 2 of 36
            Pseudo Genes (incomplete)         :: 32 of 36
            Pseudo Genes (internal stop)      :: 2 of 36
            ##Genome-Annotation-Data-END##
FEATURES             Location/Qualifiers
     source          1..6262
                     /organism="Micropruina sp."
                     /mol_type="genomic DNA"
                     /submitter_seqid="NODE_5858_length_6262_cov_4.537780"
                     /isolate="AMR_MDS_2195"
                     /isolation_source="Activated sludge of municipal
                     wastewater treatment plants"
                     /db_xref="taxon:2737536"
                     /environmental_sample
                     /geo_loc_name="Canada"
                     /lat_lon="49.25 N 123.1 W"
                     /collection_date="2015-06-16"
                     /metagenome_source="wastewater metagenome"
                     /note="metagenomic"
     gene            <1..1276
                     /locus_tag="PLF56_05520"
     CDS             <1..1276
                     /locus_tag="PLF56_05520"
                     /inference="COORDINATES: protein motif:HMM:NF018190.2"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_function: GO:0004553 - hydrolase activity, hydrolyzing
                     O-glycosyl compounds [Evidence IEA];
                     GO_function: GO:0030246 - carbohydrate binding [Evidence
                     IEA];
                     GO_process: GO:0016052 - carbohydrate catabolic process
                     [Evidence IEA]"
                     /codon_start=2
                     /transl_table=11
                     /product="sugar-binding protein"
                     /protein_id="HOQ53056.1"
                     /translation="FEITDEAATVFSSEFYAAIADGYPIDAALAEARVAVFSDNNDVE
                     WGTPVLYLRASDGRIFDVAGGAQQIPPTAATGQAEAAGEPGRVVPIVVPPWPGGGGGG
                     GGSGAGASVAGPSGGGPSGAEVSGAEAPDPLPVEPKKPGRGLRWAILAAVAAVVALAI
                     VLWPRVFPGGSVAMESPSPTGTLNASPTPTLPTTPSPSVSRTTLPPVRDVGTLTAVRV
                     TQPLTIDGKTDDWQWQLVARADQRIAGSSRATGDIYLMWDDQALYLLASVTDATPLPP
                     DAAQPSRVFRGDSVILELGPDKRRLSAADLARPTDAYYMFGLPVGGTPVIAILGPNAK
                     GTSFETSRDPRALDAAITPTNNGYVLEARIPWATTRLNGVGAGAVFAANVQVAERKAD
                     GYANLGMMSTNPQRTADVRAHPAYWQALQLQT"
     gene            1318..2523
                     /locus_tag="PLF56_05525"
     CDS             1318..2523
                     /locus_tag="PLF56_05525"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_011756222.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="DUF480 domain-containing protein"
                     /protein_id="HOQ53057.1"
                     /translation="MALPVLDASDQRILGSLLEKQRTVPGSYPLSLNSLRLACNQTSS
                     RDPITDLDDATIENALRDLKGRGLVRVVWAGKGSRTLKYHQLLDEVLSLQPDERALVT
                     VLLLRGAQSPGELKARTERLHPFDDKDDVEQVLRRMAALPEPLVRELPRRAGQREDRW
                     VHLLGPVAEPEGAAAAPSVDRELVLAQGAGARDQQVVQAYDAVADAYFDTLGDELTEK
                     PFDVWLLERIADLAGSNPIVDVGCGPGHLTAFLADTGAQVSGVDLSPAMIEVAKHEFP
                     DLDFSVGNLFTLLRPRTASGWGAIVAWYSLVHLAGSELAPAVAALARTLAPGGVVAVA
                     LHVGDEVLHVTQLVGQPSDVDAVLHDPHQVLAAFETAGLSAIEWYRRGPVGRETKTER
                     LYVLGFLAE"
     gene            3549..4301
                     /locus_tag="PLF56_05530"
     CDS             3549..4301
                     /locus_tag="PLF56_05530"
                     /inference="COORDINATES: protein motif:HMM:NF025175.2"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="DUF4184 family protein"
                     /protein_id="HOQ53058.1"
                     /translation="MPLTFVHPAAVVPLLRRRGLVPAALVIGAMTPDFEYFLRLDTVS
                     KVTHTLPGLAYGCVPLGLLALWAWQAVIRDPFVAATPTVVRDRVGHLSSSGVPHTVWM
                     WLVAGLSVLIGAATHLLWDSFTHPGYLPAQWLGLTASSPVLGLPWTSVLQRTSDLAGV
                     IVLVVVIARLPLRPVQGSHHPWSFWLVTALTTLALVAGRTLLQPDWLRLSTAVATFIA
                     GGLLGLFAAASCERRRRRSSEPAAITATDAGF"
     gene            complement(4877..5800)
                     /locus_tag="PLF56_05535"
     CDS             complement(4877..5800)
                     /locus_tag="PLF56_05535"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_012918657.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="folate-binding protein"
                     /protein_id="HOQ53059.1"
                     /translation="MNAVRHETGPDAGAVWHYGDPMREQRLLERGEATVDLSHRPVFS
                     ISGSDRLTWLNSIASQKLDDLPVGRWTTAYILDANGRIGHVFGGVDDGETLWATTEVG
                     HDQALVAWLRRMQFAARVDIAERSETHATMISFEGPEIVRRDELDEALGEARAGTWAA
                     DALRIEAGQPRIFVDTDEKTIPNELADPDGTALGPAVHLQKGCYPGQETVARVYNLGR
                     PPRRLTRLHLDGSAEALPQVGSEIVVDGRSVGRVGSSARHHELGPIALALIKRGVSPE
                     LALVVDGIDAAQELIVDPEVGLHVRPGLNRR"
     gene            complement(5797..6258)
                     /locus_tag="PLF56_05540"
     CDS             complement(5797..6258)
                     /locus_tag="PLF56_05540"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_002517150.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="FABP family protein"
                     /protein_id="HOQ53060.1"
                     /translation="MVGLAWLRGRWEGTGYREWPGEDKIRFAQQVDFTDNGGDYLHYL
                     AQAFEVDDDGRPTRPLFMETGFWRPLADATVDVVMCSPEGFAEIWYGKLQPGRLDLVT
                     DAVARTPDATVAYTAGRRLYGTVEGKLMYSFDRATETEPLRPYVWATLQRA"
BASE COUNT          951 a         2127 c         2144 g         1040 t
ORIGIN      
        1 gtttgagatc accgacgagg ccgcgaccgt gttctcgagc gagttctatg ccgcgatcgc
       61 cgacggctac ccgatcgacg ccgccctcgc cgaggcgcgg gtggcggtgt tcagcgacaa
      121 caacgacgtc gagtggggca ccccggtgct ctatctgcgg gccagtgacg gacgcatctt
      181 cgacgtcgcg gggggcgccc agcagatacc accgactgcc gcgacgggac aggctgaggc
      241 cgcgggcgag ccggggcggg tcgtcccgat cgtggtgccg ccgtggccgg ggggcggcgg
      301 tggtgggggc ggcagcggtg ccggagcctc cgttgctggc ccgtccggtg gaggaccgtc
      361 cggagccgag gtgtccggag cagaagcacc ggatccgctc cccgtcgaac cgaagaagcc
      421 gggcagaggt ctgcgttggg cgatcctggc cgcggttgca gccgtggtgg ccttggcgat
      481 cgtgctctgg cccagggtgt ttcccggtgg cagtgtcgcg atggaatcgc cctccccgac
      541 cggcacgctc aacgccagcc cgacgccgac tctgcccacc acgccgagcc cgtcggtcag
      601 ccggaccacc ctgcctccgg ttcgcgacgt gggcacgctc acagcggtcc gggtgacgca
      661 accgctcacg atcgacggca agacagacga ttggcagtgg cagttggtgg cgcgggccga
      721 ccaacggatc gcgggatcgt ccagggcgac cggcgacatc tacctgatgt gggacgacca
      781 ggcgctctac ctgctcgcgt ccgtcaccga cgcgacgccg ctgccgcccg atgccgccca
      841 gcccagccga gtctttcgcg gcgactccgt gattctcgaa ctcggacccg acaagcgccg
      901 actctcggcc gccgacctgg ccaggccgac cgacgcctac tacatgttcg gcctgccggt
      961 cggtggcacg cccgtgatcg cgatactcgg acccaacgcc aagggcacct cattcgagac
     1021 gtcgcgggac ccgcgagccc tggacgcagc aatcaccccg acaaacaacg gttacgtgct
     1081 ggaggccagg atcccgtggg cgaccacccg gctgaacggg gtaggcgccg gtgcggtgtt
     1141 cgcggcgaac gtgcaggtgg cggagcgcaa ggccgacggc tatgccaacc tcggcatgat
     1201 gagcaccaac ccgcaacgga cggcggacgt ccgggcgcat ccggcctact ggcaggcgct
     1261 gcagttgcag acgtgagttt gttcccgccc ggcgttcggt gtggttaggc tgcggccatg
     1321 gcactgcccg ttctggacgc cagcgatcaa cgcatcctcg gcagcctgct cgagaagcag
     1381 cgcaccgttc ccggctcgta tccgctcagc ctcaactccc tgcgactggc gtgcaaccag
     1441 acgtccagcc gcgacccgat caccgacctg gacgatgcca ccatcgagaa cgccctgcgc
     1501 gacctgaagg ggcgcgggct ggtccgggtg gtgtgggccg gcaaggggtc gcgcaccctc
     1561 aagtaccacc aactgctcga cgaggtgctc agcctgcaac cggacgagcg tgcgctggtc
     1621 accgtgttgc tgctgcgggg ggcccagtcg ccgggcgagt tgaaggcccg caccgagcgg
     1681 ctgcatccct tcgacgacaa ggacgacgtc gagcaggtcc tgcggcgcat ggcggcgttg
     1741 cccgaaccgt tggtgcgcga gctgccccgg cgagccggcc agcgcgagga ccgctgggtg
     1801 cacctgctcg ggccggtagc cgagccggaa ggggcggcag cggcgccgtc cgtcgaccgc
     1861 gagctggtgt tggcgcaggg cgccggggcg cgcgatcagc aggtcgtcca ggcctatgac
     1921 gcggtcgccg atgcctattt cgacaccctc ggcgacgagc tgaccgagaa gccgttcgac
     1981 gtgtggctgc tggagcgcat agccgatctt gccggctcga acccgatcgt cgacgtcggc
     2041 tgcggcccgg gtcacctcac cgccttcctc gccgacaccg gcgcccaggt gagcggggtc
     2101 gacctgtcgc cggcgatgat cgaggtggcc aagcacgagt ttcccgacct ggacttctcg
     2161 gtcggcaacc tgttcactct gctgcgtcca cgcacggcct cggggtgggg cgcgatcgtg
     2221 gcgtggtact cgctggtgca cctggccggt tcggagttgg cgccggccgt ggccgcattg
     2281 gctcgcacct tggcgcccgg cggggtggtg gcggtggccc tccacgtcgg tgacgaggtg
     2341 ctgcacgtca cccaactggt cgggcagccg tccgatgtcg acgcggtgct gcatgaccca
     2401 caccaggttt tggcggcttt cgagacggcc ggcctcagcg cgatcgagtg gtatcgccgt
     2461 ggtccggtcg gccgcgagac gaagaccgag cggctgtacg tgttggggtt cctggccgag
     2521 tgatccgagc gggcttcgca ttggtggttg agcctgtcga accacgccgt cggtcgagac
     2581 cccacccgcc ggtcgagtcc ccacccgccg gtcgaaaccc cactgccggt cgagaccaca
     2641 cccgcgggtc gaaaccacac cgcctgtcga aaccacaccg cctgtcgaaa ccgcaccgcg
     2701 ggtcaagacc tcacccaccg gtcgaaaccg caccgcgggt caagtccaca cccgccggtc
     2761 gagctggtcg agaccaggtt ggcgaccggg gtgccctgcg ccgccgtcgc atgcgacgca
     2821 tggctgcgat tctgagatcc cgacaccagt ccactccccg acccccgaca cgccggtcgt
     2881 cgccgcaacc ctggcggaag atctcagaat cctggtgatc ggtgccatga gtctctattt
     2941 cgagcagctc ctgcggctgg acaccgtgtc gaaggtccgc agactgccgg ggttggcgca
     3001 tggctgcgtg ccgctgggct tgctcgcatt gtgggcccga cgagccgtga tacgcgaacc
     3061 gatccttacg gcccgcgttc gccggaatga cggagagagc tggggccttg ggtgctgttg
     3121 ggcgggtgag attccacaag ggcccgaagg ctcaggcatc gttgcttgag ccagtcgaaa
     3181 cccttccccc tcccactccg agatgaacga gccggtcgca ctcccgagcg gaacgaaccg
     3241 gtggctgagg ctgtcgaaac ccccaccccg gtcgagacca cgttggcgac cggggtgccc
     3301 tgcactgccg tcgcatgcga cgcatggctg cgattctgaa atcccgacac cagtccactc
     3361 cccgaccccc gacacgccgg ccatcgccgc aactctggcg ggcgatttca gaaccgtcgg
     3421 cgagagtggc ccgagcagcg gcgttctgcg aggtctggac agggttgacc accattgatt
     3481 gacccggtcg aagcaccttg ccctcttcgt gaggggtctt cttggctggt tggggcacca
     3541 tgggcgaagt gccgttgacg ttcgttcacc cggcggcggt ggtgccgctg ctgcgacgcc
     3601 gtggcctggt gccggccgcc ctggtgatcg gtgccatgac ccccgacttc gagtattttc
     3661 tgcggctgga caccgtgtcg aaggtcacgc acacactgcc ggggttggcg tatggctgcg
     3721 tgccgctggg cttgctcgca ttgtgggctt ggcaagctgt gatccgcgat cccttcgttg
     3781 cggccacacc gactgtcgtc cgcgaccggg tcggccacct gagcagctca ggcgtgcccc
     3841 acaccgtgtg gatgtggctc gtggcgggtc tgtctgtact gatcggcgcg gccacgcacc
     3901 tgctctggga ttcgttcacc catcccggct atctgcctgc gcagtggctc ggcctcaccg
     3961 cgagctcgcc ggtgctcggc ctgccgtgga cctcagtgct gcagcgaacc agtgacctgg
     4021 ccggggtcat cgtgctggtg gtcgtgatcg cgcggctgcc cctacggcca gttcagggca
     4081 gccatcaccc ctggagtttc tggctggtca ccgccctcac caccctcgcg ctcgtggccg
     4141 gtcgcacgct gctgcaaccc gactggctgc ggttgtccac agcggttgcc acgttcatcg
     4201 ccggcggcct gctggggctg ttcgccgctg cgtcgtgcga gcggcggcgc cggcgatcca
     4261 gcgagccggc cgctatcacg gcaactgacg caggattctg aaatcctgcg caagggttgt
     4321 ggcggttacc ggcgtgtcgc gggtcgaggg gcggactggt gtcaagaatt cagaatcgca
     4381 gcgggtacat agtcaggaat cggcctgcgg gggctgctcg tcgctgcgtg atgcgacgag
     4441 atccgtgtcg gtacaccctg gcgagcccac tgctatcagg caactgacgc gggactctga
     4501 aatcctccgc gagggatgtg acggtcaccg gcgtgtcgca ggtcgagtag tcaactggtg
     4561 tcgagatctc agaatcgcag cgggtacctt gtcatgaaac ggcctgctgg ggctgttcgt
     4621 cgatgcgtcg tgcgagcggc ggcgtcggtg atccagcgag ccggtcgcta tcacggcaac
     4681 tgacgcagga ttctgaaatc ctccgcaagg gttgtgacgg tcaccggcgc gtcgcgggtc
     4741 gagtagtcaa ctggtgtcga gatctcagaa tcgcagcggg tacatcgtca acaagcgggg
     4801 tcccacaatt ggaccaccgg tgttcctcat ggcgaatggg cctcgacaag cgcgatcagc
     4861 gccggttgaa gcgagatcac ctgcgattca gtcccgggcg tacgtgcagg ccgacctcgg
     4921 ggtcgacgat caactcttgg gcggcgtcga ttccgtcgac caccagggcc aactccggcg
     4981 acacacctcg cttgatcagt gccaaggcga tgggacccag ctcatgatgc ctggccgacg
     5041 aaccgacccg gccgacgctg cgtccatcca ccacgatctc gctgccgacc tgtgggagcg
     5101 cctcggcaga tccgtccaga tgcagccggg tcagccggcg cggtggccgg cccaggttgt
     5161 agacccgggc gaccgtctcc tgcccggggt agcagccctt ctgcaggtgc accgccgggc
     5221 cgagcgccgt tccgtccgga tcagccaact cgttggggat cgttttctcg tcggtgtcga
     5281 cgaagatgcg cggttggccg gcctcgatgc gcagcgcatc ggccgcccac gtgccggccc
     5341 gcgcctcgcc cagcgcctcg tccaactcgt ctcgacgaac gatttccggg ccctcgaagc
     5401 tgatcatcgt ggcgtgggtt tcgcttcgct cggcgatgtc gacccgtgcc gcgaactgca
     5461 tacgccgtag ccaggccact aaagcctggt catgaccgac ctcggtggtg gcccacaggg
     5521 tttcaccgtc gtccacaccg ccgaagacgt gcccgatacg accgttcgcg tcgagaatgt
     5581 aggcggtggt ccaccgaccg actggcaggt cgtccagctt ctgcgaggcg atgctgttca
     5641 gccaggtcag ccgatccgag ccgctgatgc tgaacaccgg acggtgcgac agatcgaccg
     5701 tcgcctcgcc gcgctcgagc agtcgctgtt cgcgcatcgg gtcgccgtag tgccagacgg
     5761 cgccggcgtc gggaccggtt tcgtgccgca cggcattcat gcgcgctgca atgtcgccca
     5821 cacgtacgga cgcagtggct cggtttcggt ggcgcggtcg aaggagtaca tcagcttgcc
     5881 ctcgacggtg ccgtagaggc gccggccggc cgtataggcg acagtcgcgt cgggggtgcg
     5941 ggcgacggcg tcggtgacca ggtcgaggcg gccgggctgc agcttgccgt accagatctc
     6001 ggcgaagccc tcgggcgagc acatcacgac gtcgacggtg gcgtcggcga gcggacgcca
     6061 gaacccggtc tccatgaaca acggacgggt cgggcgcccg tcatcgtcca cctcgaacgc
     6121 ctgcgccagg tagtgcaggt agtcgccgcc gttgtcggtg aagtcgacct gctgggcgaa
     6181 cctgatcttg tcttcgcccg gccattcgcg gtagccggtg ccctcccagc gaccgcgcag
     6241 ccaggcgagc cccaccaggg cc
//
LOCUS       DAOSUG010000125         6259 bp    DNA     linear   ENV 10-JUN-2024
DEFINITION  MAG TPA_asm: Micropruina sp. isolate AMR_MDS_2195
            NODE_5862_length_6259_cov_3.338008, whole genome shotgun sequence.
ACCESSION   DAOSUG010000125 DAOSUG010000000
VERSION     DAOSUG010000125.1
DBLINK      BioProject: PRJNA925477
            BioSample: SAMN32864262
            Sequence Read Archive: SRR4236673
KEYWORDS    WGS; ENV; Metagenome Assembled Genome; MAG; Third Party Data; TPA;
            TPA:assembly.
SOURCE      Micropruina sp. (wastewater metagenome)
  ORGANISM  Micropruina sp.
            Bacteria; Bacillati; Actinomycetota; Actinomycetes;
            Propionibacteriales; Nocardioidaceae; Micropruina.
REFERENCE   1  (bases 1 to 6259)
  AUTHORS   Abdulkadir,N., Saraiva,J.P., Zhang,J., Stolte,S., Gillor,O.,
            Harms,H. and Rocha,U.
  TITLE     Genome-centric analyses of 165 metagenomes show that mobile genetic
            elements are crucial for the transmission of antimicrobial
            resistance genes to pathogens in activated sludge and wastewater
  JOURNAL   Microbiol Spectr 12 (3), e0291823 (2024)
   PUBMED   38289113
REFERENCE   2  (bases 1 to 6259)
  AUTHORS   Abdulkadir,N.
  TITLE     Direct Submission
  JOURNAL   Submitted (24-MAR-2023) Environmental Microbiology, Helmholtz
            Center for Environmental Research -UFZ, Permoserstr 15, Leipzig
            04318, Germany
COMMENT     The annotation was added by the NCBI Prokaryotic Genome Annotation
            Pipeline (PGAP). Information about PGAP can be found here:
            https://www.ncbi.nlm.nih.gov/genome/annotation_prok/
            
            ##Genome-Assembly-Data-START##
            Assembly Method        :: SPAdes v. 3.11.1
            Genome Representation  :: Full
            Expected Final Version :: Yes
            Genome Coverage        :: Not Applicable
            Sequencing Technology  :: Illumina MiSeq
            ##Genome-Assembly-Data-END##
            
            ##Genome-Annotation-Data-START##
            Annotation Provider               :: NCBI
            Annotation Date                   :: 05/04/2023 04:57:10
            Annotation Pipeline               :: NCBI Prokaryotic Genome
                                                 Annotation Pipeline (PGAP)
            Annotation Method                 :: Best-placed reference protein
                                                 set; GeneMarkS-2+
            Annotation Software revision      :: 6.5
            Features Annotated                :: Gene; CDS; rRNA; tRNA; ncRNA
            Genes (total)                     :: 2,776
            CDSs (total)                      :: 2,750
            Genes (coding)                    :: 2,714
            CDSs (with protein)               :: 2,714
            Genes (RNA)                       :: 26
            tRNAs                             :: 24
            ncRNAs                            :: 2
            Pseudo Genes (total)              :: 36
            CDSs (without protein)            :: 36
            Pseudo Genes (ambiguous residues) :: 0 of 36
            Pseudo Genes (frameshifted)       :: 2 of 36
            Pseudo Genes (incomplete)         :: 32 of 36
            Pseudo Genes (internal stop)      :: 2 of 36
            ##Genome-Annotation-Data-END##
FEATURES             Location/Qualifiers
     source          1..6259
                     /organism="Micropruina sp."
                     /mol_type="genomic DNA"
                     /submitter_seqid="NODE_5862_length_6259_cov_3.338008"
                     /isolate="AMR_MDS_2195"
                     /isolation_source="Activated sludge of municipal
                     wastewater treatment plants"
                     /db_xref="taxon:2737536"
                     /environmental_sample
                     /geo_loc_name="Canada"
                     /lat_lon="49.25 N 123.1 W"
                     /collection_date="2015-06-16"
                     /metagenome_source="wastewater metagenome"
                     /note="metagenomic"
     gene            391..627
                     /locus_tag="PLF56_05545"
     CDS             391..627
                     /locus_tag="PLF56_05545"
                     /inference="COORDINATES: ab initio
                     prediction:GeneMarkS-2+"
                     /note="Derived by automated computational analysis using
                     gene prediction method: GeneMarkS-2+."
                     /codon_start=1
                     /transl_table=11
                     /product="hypothetical protein"
                     /protein_id="HOQ53061.1"
                     /translation="MMYWFGGDVGGWGYVLMLLSMAVFWGLLITGLVLLFRVDGRQQT
                     VTPEQVLAQRFARGELDDAEYRDRLLTLRQGVRS"
     gene            624..914
                     /locus_tag="PLF56_05550"
     CDS             624..914
                     /locus_tag="PLF56_05550"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_011757210.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="DUF2892 domain-containing protein"
                     /protein_id="HOQ53062.1"
                     /translation="MTTPAPRAAHRTASQDRRRWAVNISTAERWARVVAGTAAIVGGA
                     LLLTRSGSVAAIILVLLLIAAGIDLVVTGATGHCPLYQRLGHVPRSLRRTVG"
     gene            911..1162
                     /locus_tag="PLF56_05555"
     CDS             911..1162
                     /locus_tag="PLF56_05555"
                     /inference="COORDINATES: ab initio
                     prediction:GeneMarkS-2+"
                     /note="Derived by automated computational analysis using
                     gene prediction method: GeneMarkS-2+."
                     /codon_start=1
                     /transl_table=11
                     /product="hypothetical protein"
                     /protein_id="HOQ53063.1"
                     /translation="MTVHDHHAHPEHGSNTDRAVTTPTAAHRHGGHGLMMIACCIPML
                     LIAGVLVLTGVLSPGFFLLAVACTAMMALMMTGMHDDRR"
     gene            1535..3580
                     /locus_tag="PLF56_05560"
     CDS             1535..3580
                     /locus_tag="PLF56_05560"
                     /inference="COORDINATES: protein motif:HMM:NF012514.2"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_function: GO:0016787 - hydrolase activity [Evidence
                     IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="NUDIX domain-containing protein"
                     /protein_id="HOQ53064.1"
                     /translation="MSALRAAIEEEPEPLVALTHLLHLCRDDHRVLWERVAEPVSRSR
                     VATVMDTLAEALPEGVTDARLTAPVRRAMAAALRTVDGVAPVVVAHALAQFLDDRYGH
                     TFPDSFRRRSPYQPGVGDPVPLGGTDLRVIMDMRPTSPAWRLANRLDETRRIRLAGEW
                     AVGFRIVFDYSLFEILDGVVTADTVIATCHPNRTLAEFALPQDTRQPVFPVEPADPTR
                     QLGQINRLINAATVAGADIVVLPELCVTEELAVELQDWVRRSDGPQILVAGSFHHADD
                     HGRSAGRRRRNTAVAWVRGHPDPLLHDKHSPADRPVVEDIQPDGWPELRLYVTADGWH
                     LVVAICRDLLNPQAVHAMTEAGANLVLVPAMSETLVPFGGPAAHLVGSDQALVAVANN
                     PGGWRDEPHPLTHRPGRALFGHPGFGQQTRLVHGQNTEPGIALLRVRSGQIQWIPADP
                     LAAAPRPTAPATPSWVTRLIQDVNCEPAGEQPQRPVTLRPAAVLVLLIADPTGPRVLL
                     TQRAPDLSDYPDLLVFPGGAAEPHDNGPTATAIREATEEVGLDPATVSIIGHLPMFAL
                     PDSGFLVTPVLAWSPQPGFIGGANPAEVTHVCTVALHDLGPRIGGRPILDDATSTGTG
                     PDVGTPDRMALGRITATVIDLLLATLTRADQAAVRMRTPSTMIEPPPNAYRGLEASQ"
     gene            complement(3887..4483)
                     /locus_tag="PLF56_05565"
     CDS             complement(3887..4483)
                     /locus_tag="PLF56_05565"
                     /inference="COORDINATES: protein motif:HMM:NF025016.2"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_component: GO:0016020 - membrane [Evidence IEA];
                     GO_function: GO:0009055 - electron transfer activity
                     [Evidence IEA];
                     GO_function: GO:0016491 - oxidoreductase activity
                     [Evidence IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="cytochrome b N-terminal domain-containing
                     protein"
                     /protein_id="HOQ53065.1"
                     /translation="MARRVLVVLRRLCVQGVSGSLSILIRLYGAHVVIIPGIILVLVI
                     LHLLLVKRHKMSPHPALRADASGEQASAAEPTEPFTRHLGRLAAFGVVLTGMVGMLAV
                     LFQPGIGSSPVAGIEVSDPPWMFWWPFTLENWFGLSAILYGEIAFFLILAIVPLVDRN
                     PKRWWRERPVAMILGLLVSLIVVTLTLLTAVTPAGQHL"
     gene            4671..4910
                     /locus_tag="PLF56_05570"
     CDS             4671..4910
                     /locus_tag="PLF56_05570"
                     /inference="COORDINATES: ab initio
                     prediction:GeneMarkS-2+"
                     /note="Derived by automated computational analysis using
                     gene prediction method: GeneMarkS-2+."
                     /codon_start=1
                     /transl_table=11
                     /product="hypothetical protein"
                     /protein_id="HOQ53066.1"
                     /translation="MIGSVSTIVGMLVLSLIGLWVWSMAHLSEETIQTREDHAASARI
                     GVDIPAGGIGSDSDQLGWTALDDLQLRRLLDGPSQ"
     gene            5361..>6259
                     /locus_tag="PLF56_05575"
     CDS             5361..>6259
                     /locus_tag="PLF56_05575"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_020580182.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_function: GO:0005524 - ATP binding [Evidence IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="ATP-binding cassette domain-containing protein"
                     /protein_id="HOQ53067.1"
                     /translation="MDTVVRVEGLVKSFGKFAALRGLDLQVARGEVHGFLGPNGAGKS
                     TTIRVLLGLLRADADRVELLAGDPWRDVVGLHRRLAYVPGDVSLWPGLTGGESIDLLG
                     RLRGGLDERRRARLLERFELDPTKRGRTYSKGNRQKVAIVASVWGLALRLHRGSMIGW
                     TLVFVLLGVVFGYLSTTLGGVFARNADFAAVLGARGGSAAYLTFAFLLTLLTVLGIIA
                     AVYGVQVGMRFYAEELDYRAEPLLAAALARPKLFASHAVIALLGPAAALLLGGAVIGV
                     TSSAAGGSVGAGDLIVQALAEIPA"
BASE COUNT         1042 a         2111 c         2114 g          992 t
ORIGIN      
        1 cgcgaaccac agtgggctgg catcacgcag gccccaccgg atcgccagga agcagccgcc
       61 cagcgcaacg gtgatgccga tcatgcgacc cgtccgtggt cgaccggccg acgcgatggt
      121 ccctggtgca gggacaacag gcacggtcac gctgatgcca acccttccat gatgcgcggg
      181 ggggtccccg gcgaacgccg cagcggatca gtggcaccca ttgcgaccgt acggacgctc
      241 gacactacta cggtaaatag gagcaggagg gctccccggt ctgggacgaa agtccctgcc
      301 gccaccgcga ggcacggcgg atggtgaagg tactccggag gttcccggca ccggccgtca
      361 ccgactcgga gaaggaagcg aggaacggcc atgatgtatt ggttcggtgg ggacgtcggc
      421 ggatggggct acgtcctcat gctgctcagc atggcggtgt tctggggtct gctgatcacc
      481 gggctggtcc tgctgttccg ggtcgacggc cggcagcaga ccgtgacccc cgaacaggtg
      541 ctcgcccagc ggttcgcccg gggggagctg gacgacgccg agtaccgcga ccggctgctg
      601 accctgcgcc aaggggttcg gtcatgacca cgccggcgcc ccgggcagcc caccgcacgg
      661 ccagccagga ccggcgccgc tgggcggtga acatcagcac tgccgaacgg tgggctcggg
      721 tcgttgccgg cacggccgcc atcgtcggcg gcgccctgct gctcacccgg tccggatcgg
      781 tggcggcgat catcctcgtg ctgctgctca tcgcggccgg catcgacctc gtcgtgaccg
      841 gtgcgaccgg tcactgcccg ctgtaccagc ggctcggcca cgtaccgcgg tcgctaagga
      901 ggacagtcgg atgaccgttc acgaccacca cgcacacccc gaacacggga gcaacaccga
      961 ccgcgccgtc accacgccga ccgccgccca tcggcacggc gggcacgggc tgatgatgat
     1021 cgcctgctgc atccccatgc tgctgatcgc cggtgtgctg gtgctcaccg gcgtcctgtc
     1081 tcccggcttc ttcctcctgg cggtcgcctg caccgcgatg atggcgctga tgatgacggg
     1141 catgcacgac gaccgacgct gaacgttcca acgaaacaac tgatagtgaa aggcgtacga
     1201 gactgaagtg cccagtgtgc cgaacgcgcg gaagtgctct ggccggcgcc gccgagcggc
     1261 tccttcgcgt tcgtttaccg cgggtggacc gatgaatggg gggcaccctg accccggtgt
     1321 ggcgggctac ctaccggagc gcgccctcgc cgatgcccga caagcaggcg cgccagcacc
     1381 cactcccggg tactcggccc cggcggctgg tcaaagcctt cggcgggcca tggcccccct
     1441 accccaggcg aacccgctct ccgctccccc tcctggtgag cagggaatca cctgcggacc
     1501 ctgtgtggct cggttatcgt ccccggccga ccggatgagt gcgttgcgcg ccgcgatcga
     1561 agaggagccc gagccgttgg tggcgctcac ccatctgctg catctgtgcc gtgacgacca
     1621 ccgggtgtta tgggagcggg tggccgagcc ggtcagccgc tcccgagtgg cgaccgtcat
     1681 ggacactttg gctgaggcgc tgcctgaggg tgtgaccgac gcgaggctga ccgcgccggt
     1741 tcgccgcgcg atggccgccg ctctgcggac cgtggacggc gtggcaccgg tcgtggtcgc
     1801 gcacgcgctc gcgcagttcc tcgacgaccg ttacggtcac accttcccgg actcgtttcg
     1861 tcgccgcagc ccataccagc ctggggtcgg ggacccggtc ccgctagggg gcacggacct
     1921 gcgtgtgatc atggacatgc ggcccacctc accggcgtgg cggctggcga accggttgga
     1981 tgagacgcgg cggatccggt tggccggtga gtgggcggta ggttttcgga tcgtcttcga
     2041 ctacagcctg ttcgagattc tggacggcgt ggtgacggcg gacacggtca ttgcgacgtg
     2101 ccaccccaac cgcaccttgg ccgagttcgc tctgccgcag gacacccgac agccggtgtt
     2161 tccggtggaa ccggccgatc cgacccgaca gcttggccag atcaaccggc tgatcaacgc
     2221 tgccacagtg gcgggggccg atatcgtggt gctccccgag ttgtgtgtca ccgaggagct
     2281 cgccgtcgaa ttgcaggact gggtgcgccg ctcggatggc ccccaaatcc tggtggcggg
     2341 cagcttccat cacgccgatg accacggccg atcggccggt cgccggcgcc gcaacaccgc
     2401 tgtcgcgtgg gttcgcggcc atccggaccc gctccttcat gacaagcact cgcccgcgga
     2461 ccgcccggtc gtggaagaca tccagcccga cggttggccc gaactgcggc tgtacgtcac
     2521 cgctgacggc tggcacctgg tggtcgccat ctgccgcgac ttgctgaacc cacaggccgt
     2581 gcacgccatg accgaggccg gcgcgaatct ggtgctggtt ccggcgatga gcgagaccct
     2641 cgtgcccttc ggcggtcccg cggcccacct ggtcggctcg gaccaggccc tcgtcgccgt
     2701 ggctaacaac cccggcgggt ggcgcgacga gcctcaccca ctcacccacc ggcccggccg
     2761 tgcactgttc ggccatcctg ggttcggtca acagacccgg ctcgtccatg gccagaacac
     2821 cgagccgggg atcgccctgt tacgggtgcg atccggtcag atccagtgga tccccgccga
     2881 tccgctcgcg gcggcgccac gaccgaccgc gccggcgact ccctcctggg tcacccgact
     2941 gatccaggac gtcaactgcg aaccggctgg tgagcagccg caacgaccag tcaccctgcg
     3001 cccggcggcg gtgttggtcc tgctcatcgc cgacccgacc ggtccccgag tgttgctgac
     3061 ccaacgcgca cccgacctga gtgactatcc cgacctgctg gtgttccccg gcggcgccgc
     3121 cgagccgcac gacaacggcc caacggcgac ggctataagg gaagccaccg aagaagtggg
     3181 gctggacccg gccaccgtgt cgatcatcgg acacctgccg atgttcgccc ttccggacag
     3241 cggcttcctg gtcaccccag tgctggcctg gtccccgcag cccgggttca tcggaggcgc
     3301 caatcccgct gaggtcaccc acgtgtgcac cgtcgcactg cacgacctcg gcccccgtat
     3361 cggtggccgg cccattctgg acgatgcgac cagcacagga acaggcccgg acgtggggac
     3421 acctgaccgt atggccctcg gtcggatcac cgcaaccgtg atcgacctgc tgctagcgac
     3481 tctcacccgc gccgaccagg ccgcggtccg gatgcggaca ccatcaacca tgatcgaacc
     3541 tccaccaaat gcttaccgcg gccttgaagc ctctcaatga ccggcgatac caatatcggt
     3601 gctcaaccgt tcggcttccc tttccctccc accgactctc ggccggctcg ggcggcccgg
     3661 cgcgcccact ggatggcgag cagcacccgg gcggcgaggg ccaggcagac gataaggagc
     3721 acgctggcgc ccgcgaagca gacagccatg agcgggccag gcgccgcagc gaatgaagca
     3781 ttgacggctc ccgccatgag aaccgcgatg cccaccagga cccagaacga gatataggca
     3841 cgaggcggtg ggttgcgggc ccagcggccc ggcgccactt gcgcattcat aggtgttgtc
     3901 ctgctggggt gacggcggtc aggagtgtca aagtgaccac aatcagcgat accaatagac
     3961 ccaggatcat cgcgaccgga cgctcgcgcc accagcgttt cggattacgg tcaacaagag
     4021 gcacgatcgc gaggatgagg aagaatgcaa tctcaccgta aaggatggcg gaaagcccaa
     4081 accagttctc tagggtgaac ggccaccaaa acatccacgg cgggtcggac acctcgatgc
     4141 ccgccacggg ggaggatccg atcccgggtt ggaagagaac cgcgagcatg cccaccatcc
     4201 cggtcaacac caccccgaag gccgcgagcc gacccagatg gcgggtgaac ggttccgtcg
     4261 gctccgccgc tgaggcttgt tcgccggagg catcggcacg cagggccggg tgcggagaca
     4321 tcttgtgccg cttcaccaga agaagatgca gaatcaccag aacaagaatg attcccggga
     4381 tgatcaccac atgggcgcca tagaggcgta tcaagatgga gagagatccg ctgacaccct
     4441 gtacacagag acggcgaagc acaacaagca cgcgccgcgc cacgggacag tccgcgatat
     4501 gactgatggt gagacattca cccaggcacc acttgcgacg ccaacgcgag accctgggac
     4561 gagcgtacaa ccgccggcga ggatctgagc accgagtacc gggctgcaga gtcaagttca
     4621 cgttaagagc gcactcattt gaataacgac ggctaaggag ttgaggcgcg atgatcgggt
     4681 cggtgtcgac gatcgtcggc atgctggtcc tgtcactcat cggtctatgg gtctggtcaa
     4741 tggcccacct atcggaggag accatccaga ctcgcgaaga tcacgcggct tctgcccgca
     4801 tcggggtcga catccccgcc gggggcatcg gctctgattc agaccagctc gggtggaccg
     4861 cgctggacga tctacaactc cgccggctac tcgatggacc ctcacagtga ggttcctcga
     4921 cttgcactcg gtcgcagggg tgtgtgttga ccgttgtcgg aactgccgac caaggagtcg
     4981 ccatggttga cgacggggga tcggtgtttg gtcggcatga ggccggcggg cggcgccgac
     5041 gagcaggttc aggggcgtca accgaggggt catggccagc agcatgccga ccaacaggtc
     5101 ccggtcgttg gtgactgctg agcatcgacc cgagccgtta cagccaccgg gcgtgtcatc
     5161 gccggtgggg cccgcatcca ccgtcgaacg cgactcgcca gtcaccgaat cgaaagggtc
     5221 atctgccaca tcagctatat tgtccagcga atgccgcgaa catatgctcg ccgcaccccg
     5281 cacccccgca tcggacaacg gaccaaataa cgctccatcc tggaaggcag aagaacaaat
     5341 cgggcgtacc ctctaggggt atggatacgg tggtccgggt cgagggcctg gtcaagtcct
     5401 tcggcaaatt cgcggcgctg cggggcctgg accttcaggt cgcccggggc gaggtgcatg
     5461 gcttcctggg gccgaacggg gcgggtaagt cgaccaccat ccgggtgctg ctggggctgc
     5521 tccgggcgga tgcggatcgg gtcgagttgt tggccggtga tccgtggcgg gacgtggtcg
     5581 ggttgcaccg ccgcctggcg tacgtgccgg gggatgtcag cctgtggccg ggtttgaccg
     5641 gtggggagag catcgacctg ctgggtcggt tgcggggcgg attggacgag cggcgccgcg
     5701 cccgcctgtt ggagcggttc gagctggacc cgacgaagcg gggccggacg tattccaagg
     5761 gcaaccggca gaaggtggcg atcgtcgcct cggtgtgggg gttggcgctg cggctgcacc
     5821 gcggctcgat gatcggctgg acgctggtgt tcgtgctgct cggcgtggtt ttcgggtacc
     5881 tgtcgaccac cttgggtggg gtgttcgccc ggaacgccga cttcgctgcg gtgctcggcg
     5941 cccggggtgg gtccgcggcg tacctgacgt tcgcgttcct gctgaccctg ctcaccgtgc
     6001 tcggcatcat cgccgccgtc tacggcgtgc aggtcgggat gcggttctac gccgaggaac
     6061 tcgactaccg ggcggaaccc ctgctcgctg cggcgctggc ccgcccgaag ctgttcgcca
     6121 gccacgcggt gatcgccctg ctcggcccgg cggcggcgtt gctgctgggc ggggcggtga
     6181 tcggcgtgac gtcgtccgcg gccggcggct cggtcggtgc cggtgacctg atcgtgcagg
     6241 cgctggccga gatccccgc
//
LOCUS       DAOSUG010000126         6210 bp    DNA     linear   ENV 10-JUN-2024
DEFINITION  MAG TPA_asm: Micropruina sp. isolate AMR_MDS_2195
            NODE_5910_length_6210_cov_3.778716, whole genome shotgun sequence.
ACCESSION   DAOSUG010000126 DAOSUG010000000
VERSION     DAOSUG010000126.1
DBLINK      BioProject: PRJNA925477
            BioSample: SAMN32864262
            Sequence Read Archive: SRR4236673
KEYWORDS    WGS; ENV; Metagenome Assembled Genome; MAG; Third Party Data; TPA;
            TPA:assembly.
SOURCE      Micropruina sp. (wastewater metagenome)
  ORGANISM  Micropruina sp.
            Bacteria; Bacillati; Actinomycetota; Actinomycetes;
            Propionibacteriales; Nocardioidaceae; Micropruina.
REFERENCE   1  (bases 1 to 6210)
  AUTHORS   Abdulkadir,N., Saraiva,J.P., Zhang,J., Stolte,S., Gillor,O.,
            Harms,H. and Rocha,U.
  TITLE     Genome-centric analyses of 165 metagenomes show that mobile genetic
            elements are crucial for the transmission of antimicrobial
            resistance genes to pathogens in activated sludge and wastewater
  JOURNAL   Microbiol Spectr 12 (3), e0291823 (2024)
   PUBMED   38289113
REFERENCE   2  (bases 1 to 6210)
  AUTHORS   Abdulkadir,N.
  TITLE     Direct Submission
  JOURNAL   Submitted (24-MAR-2023) Environmental Microbiology, Helmholtz
            Center for Environmental Research -UFZ, Permoserstr 15, Leipzig
            04318, Germany
COMMENT     The annotation was added by the NCBI Prokaryotic Genome Annotation
            Pipeline (PGAP). Information about PGAP can be found here:
            https://www.ncbi.nlm.nih.gov/genome/annotation_prok/
            
            ##Genome-Assembly-Data-START##
            Assembly Method        :: SPAdes v. 3.11.1
            Genome Representation  :: Full
            Expected Final Version :: Yes
            Genome Coverage        :: Not Applicable
            Sequencing Technology  :: Illumina MiSeq
            ##Genome-Assembly-Data-END##
            
            ##Genome-Annotation-Data-START##
            Annotation Provider               :: NCBI
            Annotation Date                   :: 05/04/2023 04:57:10
            Annotation Pipeline               :: NCBI Prokaryotic Genome
                                                 Annotation Pipeline (PGAP)
            Annotation Method                 :: Best-placed reference protein
                                                 set; GeneMarkS-2+
            Annotation Software revision      :: 6.5
            Features Annotated                :: Gene; CDS; rRNA; tRNA; ncRNA
            Genes (total)                     :: 2,776
            CDSs (total)                      :: 2,750
            Genes (coding)                    :: 2,714
            CDSs (with protein)               :: 2,714
            Genes (RNA)                       :: 26
            tRNAs                             :: 24
            ncRNAs                            :: 2
            Pseudo Genes (total)              :: 36
            CDSs (without protein)            :: 36
            Pseudo Genes (ambiguous residues) :: 0 of 36
            Pseudo Genes (frameshifted)       :: 2 of 36
            Pseudo Genes (incomplete)         :: 32 of 36
            Pseudo Genes (internal stop)      :: 2 of 36
            ##Genome-Annotation-Data-END##
FEATURES             Location/Qualifiers
     source          1..6210
                     /organism="Micropruina sp."
                     /mol_type="genomic DNA"
                     /submitter_seqid="NODE_5910_length_6210_cov_3.778716"
                     /isolate="AMR_MDS_2195"
                     /isolation_source="Activated sludge of municipal
                     wastewater treatment plants"
                     /db_xref="taxon:2737536"
                     /environmental_sample
                     /geo_loc_name="Canada"
                     /lat_lon="49.25 N 123.1 W"
                     /collection_date="2015-06-16"
                     /metagenome_source="wastewater metagenome"
                     /note="metagenomic"
     gene            160..516
                     /locus_tag="PLF56_05580"
     CDS             160..516
                     /locus_tag="PLF56_05580"
                     /inference="COORDINATES: protein motif:HMM:NF022700.2"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="DUF3054 domain-containing protein"
                     /protein_id="HOQ53068.1"
                     /translation="MKWWTALCFDLVVVLAFATIGRLSHGEAADPGGIVNTAWPFVVA
                     LIGTTGILIGLQRPTELVTNGALVWVGTLVFGMWIRARSGDGVQVSFVIVAGVFLVLC
                     MLGWRLLAARRAHAAR"
     gene            complement(830..2446)
                     /gene="arc"
                     /locus_tag="PLF56_05585"
     CDS             complement(830..2446)
                     /gene="arc"
                     /locus_tag="PLF56_05585"
                     /EC_number="5.6.1.5"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_013863501.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_component: GO:0000502 - proteasome complex [Evidence
                     IEA];
                     GO_function: GO:0016887 - ATP hydrolysis activity
                     [Evidence IEA];
                     GO_process: GO:0010498 - proteasomal protein catabolic
                     process [Evidence IEA];
                     GO_process: GO:0019941 - modification-dependent protein
                     catabolic process [Evidence IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="proteasome ATPase"
                     /protein_id="HOQ53069.1"
                     /translation="MSEGPADAVQRAEAVAQARGRAAELAERRLADARADARSLALQN
                     EKLNATLREARESLLQLRAQLDALANPPQAHGVVIGVPEVGLADVSVGGRVLRVAVGP
                     DVDPGGLRPGAAVLLNEAMMLVANAPEAAQLDVVVVRDVLADGRLLVGGGGDDEHLLT
                     AGPDLTGVQPGQSVAADRRAGIALRVVERSDVNDLLLDEVPDLDYADIGGLGRQIEQI
                     RDAVELPFLHRDLFARYQLPAPKGVLLYGPPGCGKTMIAKAVANSLGDQLGGVGSHFL
                     NIKGPELLNKYVGETERQIRVIFATAREHAADGRPVIVFFDEMDSLFRTRGSGVSSDM
                     ESTVVPQLLSEIDGVESLSNVIIIGATNREDLIDPAILRPGRLDVKIKLDRPDSAAAA
                     EILAKYLTEAIPLSARQSGGDPVAQRRALIDATVEQLYRRSDANRFLEVTYASGDREV
                     LYVSDFVSGAMLANVVGRAKKAAIKDVLAGRPDGLLLEHLLEACADEVAENEDLPNTT
                     NPDDWARISGRKGERITFMRTLRGGRQVSV"
     gene            complement(2443..3354)
                     /locus_tag="PLF56_05590"
                     /pseudo
     CDS             complement(2443..3354)
                     /locus_tag="PLF56_05590"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_002534941.1"
                     /note="incomplete; partial in the middle of a contig;
                     missing N-terminus; Derived by automated computational
                     analysis using gene prediction method: Protein Homology."
                     /pseudo
                     /codon_start=1
                     /transl_table=11
                     /product="tRNA (adenine-N1)-methyltransferase"
     gene            complement(3384..4892)
                     /locus_tag="PLF56_05595"
     CDS             complement(3384..4892)
                     /locus_tag="PLF56_05595"
                     /inference="COORDINATES: protein motif:HMM:NF012706.2"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="ABC transporter substrate-binding protein"
                     /protein_id="HOQ53070.1"
                     /translation="MPPLTLPSFVRSLVALVTACGTMVAASGCIRTPTPLPTTSVVSP
                     SPDRVFTVMTAQKATTTDPAGARNDSDAMVILDLFQRLMLVQPSTQLLKPDLAKECLY
                     TAPTIFECSLPRNVVFSNGHQLTSSDVKFSLQRALRLAPGQLSLLGALRSIETPDDWT
                     VRFRLKYPDNQFGYGLAVPAASVVDEEAYSATKLRPVDQMPMGTGPYSLEVWDNRGLT
                     IDKFANYVGPLGTDMPRIRITWADSPTAEEALTAQQVEIVWRTLSPAAVDRLQARIRQ
                     ALADNERPAFYRWGLTGTVLQRLVWVADSKYRPNATLRAGIALALQDDRTATSILPTI
                     IDSHTDSFAVGGNPKVPSLPKGINLTLRFTNSAPGMADLARQVSDRLHERLGISVRLV
                     TEGEAADLELRDSAPQVNTALGWLQDYLKNPLPGSAGKLAELEERIRSSSKEDVQLPA
                     LSEIQKQAAVDATVVPIAEGDGVLFLGARVDLVGEALGPGWQLALWGARWRN"
     gene            4991..>6210
                     /locus_tag="PLF56_05600"
     CDS             4991..>6210
                     /locus_tag="PLF56_05600"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_013863505.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="homocysteine S-methyltransferase family protein"
                     /protein_id="HOQ53071.1"
                     /translation="MSSDLLTTIPSTPAEIRPDLTEHLRAALATRMHFLDGAMGTMIQ
                     RFGLDESDYRGERFAAWGQDVKGNNDLLSLTQPEIIASIHRSYLEAGADFIETNTFNA
                     QRISMADYGMQQLAYELNLASARLARSACDEFTAADPSRPRYVIGALGPTSKTASISP
                     DVNDPGARNVQYAELVEAYLEQAEGLVDGGADLLMIETIFDTLNAKAAVFALETLFEQ
                     RGRRWPVILSGTITDASGRTLSGQVTEAFYNSIRHARPLAVGLNCALGAAEMRPYIAE
                     LSRVADCFVSCYPNAGLPNAFGEYDQTPAEMAAIVAGFAADGLVNVLGGCCGTTPEHI
                     AAVVAAARESAPRRPAEPVPAMRLAGLEPLTITPDSLFVNVGERTNITGSARFRRLIR
                     EGDYTTALDVARQQV"
BASE COUNT          947 a         2198 c         2088 g          977 t
ORIGIN      
        1 gacccgaccc cgtgtgcccg ggcccgggta tccgtccacc accgcggccg agaccgcgct
       61 ggggtgcagg tcgggccggc ccggcgccag aatgccgtac tcggtctcgg ctcccagcaa
      121 cggcatgtcc cacagcgtag ggcccataca gtggtcggca tgaagtggtg gacggccctg
      181 tgcttcgacc tcgtggtcgt gctcgctttc gcgaccatcg gacgcctgtc gcatggcgag
      241 gcggccgacc ccggcggaat agtgaacact gcatggccgt tcgtggtggc actgatcggc
      301 accaccggca tcctgatcgg cctgcagcgc cccaccgagt tggtgacgaa cggtgcactg
      361 gtatgggtag gaacgctggt gttcggcatg tggattcgcg cacgctccgg tgacggcgtt
      421 caggtgagct tcgtgatcgt ggcgggcgtt ttcctggtcc tgtgcatgct cggctggcgc
      481 ctgctggcgg cccggcgagc gcacgccgct cgctgattgt cgacgagttc aaccgccaac
      541 ccggcgagtt caaccgtcct cccgcgagtt cacccgccat cccgcccaac ttgatcggct
      601 tcccggcgag ttcaaccatc atcccggcga acgccgggat ctcctgcctc gcgcccaccc
      661 gcccaccgac acacagcgtt gaggtcaccg gggcgacagt agacgtcgcc gggcggcagt
      721 cgagcgcgtc agagatcccg gcgttcgccg ggatgacgac tcagcaggag gacggccgag
      781 cggatgaccg tgtagtccgc cggaatgaca gctgtgctcg ccgctaggcc tacacgctca
      841 cctggcgtcc gccgcgcagg gtgcgcatga aggtgatccg ctcccccttg cggcccgaga
      901 tccgggccca gtcgtcgggg ttggtggtgt tcggcaggtc ctcgttctcg gccacctcgt
      961 cggcgcaggc ctccagcaga tgttccagga gcagcccgtc cgggcggccc gccagcacgt
     1021 ccttgatcgc ggccttcttg gcccggccga cgacgttggc cagcatcgcc cccgacacga
     1081 agtcgctgac gtacagcacc tcgcggtccc cggaggcgta ggtgacctcg agaaaccggt
     1141 tggcgtcact gcgccggtac agctgctcga cggtggcgtc gatcagcgcc cggcgctggg
     1201 ccaccgggtc gccgcccgac tgccgggccg agagcgggat ggcctcagtc aggtacttgg
     1261 ccagaatctc ggccgccgcc gcgctgtccg gacggtccag tttgatcttc acgtcgagtc
     1321 tgcccggacg cagaatggcc gggtcgatca gatcctcacg gttggtggcc ccgatgatga
     1381 tcacgttcga caggctctcg acaccgtcga tctcgctcag caactggggc accacggtcg
     1441 attccatgtc ggaactgacg ccgctgccgc gggtgcggaa cagcgaatcc atttcgtcga
     1501 agaacacgat caccggacgt ccgtcggccg cgtgttcgcg ggccgtggcg aagatcaccc
     1561 ggatctgccg ctcggtctcg ccgacatact tgttgagcag ttcgggaccc ttgatgttga
     1621 gaaagtgact gcccacgccg cccagctggt cacccagcga gttggccacc gccttcgcga
     1681 tcatcgtctt gccgcaaccg ggcggtccgt acagcagcac gcccttcggt gcgggcaact
     1741 ggtagcgggc gaacaggtcg cggtgcagaa acggcagctc gacggcgtcg cggatctgct
     1801 cgatctgccg gcccaggccg ccgatgtcgg cgtagtcgag gtcgggcacc tcgtcgagca
     1861 gcaggtcgtt gacgtcgctg cgctccacca cgcgcaacgc gatgccggcc ctacgatcgg
     1921 cggcgacact ctgccccggc tgcacgccag taaggtccgg gcccgcggtg agcaggtgct
     1981 cgtcgtcgcc gccgccgccg accagtagcc gtccgtctgc cagcacgtcg cgcaccacca
     2041 ccacgtccaa ctgggcggcc tcgggtgcgt tcgcgaccag catcatcgcc tcgttgagca
     2101 acaccgccgc gcctggacgc agcccgcccg ggtccacgtc ggggccgacg gccacgcgaa
     2161 gcacccggcc accgaccgac acgtcagcca atcccacctc gggcacgccg atcaccaccc
     2221 cgtgcgcctg tggcgggttc gccagcgcgt ccagttgcgc ccgcagctgc agcaaggact
     2281 cgcgagcctc acgcagcgtc gcgttcagct tctcgttctg cagtgcgagt gagcgggcat
     2341 cggcacgcgc atcggccagc cgtcgctcgg ccagttcggc agcccgtcca cgcgcctgcg
     2401 ccaccgcctc agcacgctgc accgcatcgg ccgggccctc gctcatgacg gggtctcgga
     2461 cgccggcgcc tccgcggcct gatccaccgg cacctcgacg ccgggcggac ggggcccgcg
     2521 atagtccggc ccgtaggccc cgggtgaggg acggcgccgg cgcagtggtg cggtgacccc
     2581 gggggccagc cgacgtgtga agatcaagaa gccggtgtga cccgtggtgg cgtgacccgg
     2641 acggatggcc agtccctcgg cgtgccactg ccgcgacgtc agctcgctgc ccgccggctc
     2701 ggtgaagccg ccatgggcgc ggatcgtgtc catggtgcgg cccagttggg tggtggtggc
     2761 gacgtagcag cacagcatgc cgccggcttc gagcacgcgc gcgaccgcgt cgatgcattc
     2821 ccacggcgcg agcatgtcga gaatcgcgcg atcgatgggt tcgggcccga tgtcagcggc
     2881 caggtcgccg acccggatct gccacgcggg gtgcgggccg ccgaagaacg tctcgacgtt
     2941 gcgctgcgcg gtcgccgcga aatcgggacg ccgctcgtag ctgtacagcg tgcccgtcgg
     3001 gccgatgctg cgcagcagat gcatgctcaa cgcacccgag ccgacccccg cctcgagcac
     3061 ccgggcccct gggaagatgt cggtctgcac cagaatgtgg gcggcgtcct tggggtagat
     3121 gacggccgcg ccgcgcggca tcgacaccgt ccattcctcc agcagcggac gaaaggccac
     3181 ataccccatg ccgccggccg aggtgaccac gttgccgtcg ggcccgccga tcaggtcgtc
     3241 gtggctgatc gcgccccggg tggtgtgaaa ggtgccgccg gtgaccagca gcaccgaatg
     3301 ccgccggccc ttggggtcac tcagcgtgac ccgctcaccc gctcgcaacg gtccggtgtg
     3361 cacaccggtg agccgcgcct cgttcagttc cgccatcgtg ctccccacaa cgccaattgc
     3421 catccagggc cgagcgcctc gccgaccaga tcgacccgtg cacccaggaa cagcaccccg
     3481 tcgccctcgg cgatcggcac caccgtggcg tccacggccg cctgcttctg aatctcactg
     3541 agcgcgggca actgcacgtc ttctttgctg gaggaccgga tgcgctcctc cagttcggcc
     3601 agcttgccgg cgctgccggg caacgggttc ttcagataat cctgcaacca acccagtgcc
     3661 gtgttcacct ggggggcgct gtcgcgcagt tcgagatcgg ccgcttcgcc ttcggtgacc
     3721 aggcggacgc tgatacccag ccgttcgtgc agccggtcac tcacctggcg ggccaggtcg
     3781 gccatgcccg gtgccgaatt ggtaaacctc aacgtgaggt tgatgccctt gggcaggctc
     3841 ggcaccttgg ggttgccgcc caccgcaaag ctgtcggtgt ggctgtcgat gatggtcggc
     3901 aggatcgagg tggcggtgcg atcgtcctgc agggcgagcg cgatgccggc ccgcagggtg
     3961 gcgttggggc ggtacttgct gtcggccacc cacaccaggc gctgcagcac ggtgccggtc
     4021 aggccccagc ggtagaacgc cggacgttcg ttgtccgcca gcgcttgccg gatgcgcgcc
     4081 tgcagccggt ccacggctgc aggactgagc gtgcgccaca cgatctcgac ctgctgcgcg
     4141 gtgagcgcct cttcggcggt cggtgaatcg gcccaggtga tgcggatcct gggcatgtcg
     4201 gtgccgaggg gtcccacgta gttggcgaac ttgtcgatgg tcagcccgcg gttgtcccag
     4261 acctcgagcg agtagggccc ggtgcccatc ggcatctggt cgacggggcg cagcttggtc
     4321 gccgagtacg cctcttcgtc caccacggac gccgccggca ccgccaggcc gtacccgaac
     4381 tggttgtcgg ggtacttcag ccgaaaccgc accgtccaat cgtccggggt ctcgatgctg
     4441 cgcagagcgc ccagcaacga cagctgaccc ggcgccagcc gcagcgcacg ctgcaagctg
     4501 aacttgacgt cagaactggt gagctggtga ccgttggaga acaccacgtt cctgggcagg
     4561 gagcattcaa agatggtcgg ggcggtgtac aggcactcct tggccaggtc gggtttgagc
     4621 agctgggtgc tcggctgcac cagcatcaga cgctggaaca ggtcgaggat caccatcgcg
     4681 tcggagtcgt tgcgcgcccc ggccggatcg gtcgtggtcg ccttctgcgc ggtcatcacc
     4741 gtgaacaccc ggtcgggact ggggctgacc acgctggtgg tgggcaacgg cgtgggggtg
     4801 cgaatgcagc cgctcgccgc gaccattgtg ccgcaggcgg tgaccaacgc gaccagtgac
     4861 cggacgaacg agggcagggt cagcggcggc acctcactag ggtaggggtt ccgtccactg
     4921 agcgcctgat ctcggccgca gtcgcgcctc gcgtacagtg agccagttca tagcgacgaa
     4981 gggagctgcg gtgagttctg acctgctcac aacgatcccg tcgaccccgg ccgagatccg
     5041 tccggacctc accgagcacc tgcgcgccgc actcgccacg cgcatgcact ttctggacgg
     5101 ggccatgggc accatgatcc agcggttcgg gctggacgag tccgactacc gcggtgagcg
     5161 gttcgcagcc tggggccagg acgtcaaggg caacaacgac ctgctctcgc tcacccagcc
     5221 cgagatcatc gcgtcgatcc accgcagcta cctcgaggcc ggcgccgact tcatcgagac
     5281 gaacaccttc aacgcgcaac gcatctcaat ggccgactac ggcatgcagc aactcgccta
     5341 cgagctgaat ctggcctcgg cgcggctggc ccgctcagcc tgcgacgagt tcacggcggc
     5401 cgacccgtcc cggccccgct acgtgatcgg ggcgctgggc ccgaccagca agaccgcgtc
     5461 catctcgccc gacgtcaacg atccgggcgc ccgtaacgtg cagtacgccg agttggtcga
     5521 ggcctacctg gagcaggccg agggcctggt cgacggcggc gccgacctgt tgatgatcga
     5581 aaccatcttc gacacgctca acgccaaggc ggcggtcttc gccctcgaaa ccctgttcga
     5641 gcagcgcgga cgccgctggc cggtgatcct gtccggcacc atcaccgacg cctccggacg
     5701 caccctgtcg ggccaggtca ccgaggcctt ctacaactcg atccggcacg ccaggccgct
     5761 ggcggtgggg ctgaactgcg cgctcggcgc ggccgagatg cgtccctaca tcgcggaact
     5821 gtcgcgggtg gcggactgtt tcgtgagctg ctaccccaac gccggactgc ccaacgcctt
     5881 cggcgagtac gaccagaccc cggccgagat ggcggcgatc gtggcgggtt tcgctgcgga
     5941 cgggctggtg aacgtgttgg gcggctgttg cggcaccacc cccgagcaca tcgcggcggt
     6001 cgtcgccgcg gcgcgcgaat cggcaccccg acgaccggcg gaaccggtgc cggcgatgcg
     6061 cctggccggc ctggagccgc tcaccatcac cccggactcg ctgttcgtca acgtcggcga
     6121 gcgcaccaac atcaccggct cggcccggtt ccgccggctg atccgcgagg gcgactacac
     6181 caccgcgctc gacgtggccc ggcagcaggt
//
LOCUS       DAOSUG010000127         6204 bp    DNA     linear   ENV 10-JUN-2024
DEFINITION  MAG TPA_asm: Micropruina sp. isolate AMR_MDS_2195
            NODE_5916_length_6204_cov_4.855098, whole genome shotgun sequence.
ACCESSION   DAOSUG010000127 DAOSUG010000000
VERSION     DAOSUG010000127.1
DBLINK      BioProject: PRJNA925477
            BioSample: SAMN32864262
            Sequence Read Archive: SRR4236673
KEYWORDS    WGS; ENV; Metagenome Assembled Genome; MAG; Third Party Data; TPA;
            TPA:assembly.
SOURCE      Micropruina sp. (wastewater metagenome)
  ORGANISM  Micropruina sp.
            Bacteria; Bacillati; Actinomycetota; Actinomycetes;
            Propionibacteriales; Nocardioidaceae; Micropruina.
REFERENCE   1  (bases 1 to 6204)
  AUTHORS   Abdulkadir,N., Saraiva,J.P., Zhang,J., Stolte,S., Gillor,O.,
            Harms,H. and Rocha,U.
  TITLE     Genome-centric analyses of 165 metagenomes show that mobile genetic
            elements are crucial for the transmission of antimicrobial
            resistance genes to pathogens in activated sludge and wastewater
  JOURNAL   Microbiol Spectr 12 (3), e0291823 (2024)
   PUBMED   38289113
REFERENCE   2  (bases 1 to 6204)
  AUTHORS   Abdulkadir,N.
  TITLE     Direct Submission
  JOURNAL   Submitted (24-MAR-2023) Environmental Microbiology, Helmholtz
            Center for Environmental Research -UFZ, Permoserstr 15, Leipzig
            04318, Germany
COMMENT     The annotation was added by the NCBI Prokaryotic Genome Annotation
            Pipeline (PGAP). Information about PGAP can be found here:
            https://www.ncbi.nlm.nih.gov/genome/annotation_prok/
            
            ##Genome-Assembly-Data-START##
            Assembly Method        :: SPAdes v. 3.11.1
            Genome Representation  :: Full
            Expected Final Version :: Yes
            Genome Coverage        :: Not Applicable
            Sequencing Technology  :: Illumina MiSeq
            ##Genome-Assembly-Data-END##
            
            ##Genome-Annotation-Data-START##
            Annotation Provider               :: NCBI
            Annotation Date                   :: 05/04/2023 04:57:10
            Annotation Pipeline               :: NCBI Prokaryotic Genome
                                                 Annotation Pipeline (PGAP)
            Annotation Method                 :: Best-placed reference protein
                                                 set; GeneMarkS-2+
            Annotation Software revision      :: 6.5
            Features Annotated                :: Gene; CDS; rRNA; tRNA; ncRNA
            Genes (total)                     :: 2,776
            CDSs (total)                      :: 2,750
            Genes (coding)                    :: 2,714
            CDSs (with protein)               :: 2,714
            Genes (RNA)                       :: 26
            tRNAs                             :: 24
            ncRNAs                            :: 2
            Pseudo Genes (total)              :: 36
            CDSs (without protein)            :: 36
            Pseudo Genes (ambiguous residues) :: 0 of 36
            Pseudo Genes (frameshifted)       :: 2 of 36
            Pseudo Genes (incomplete)         :: 32 of 36
            Pseudo Genes (internal stop)      :: 2 of 36
            ##Genome-Annotation-Data-END##
FEATURES             Location/Qualifiers
     source          1..6204
                     /organism="Micropruina sp."
                     /mol_type="genomic DNA"
                     /submitter_seqid="NODE_5916_length_6204_cov_4.855098"
                     /isolate="AMR_MDS_2195"
                     /isolation_source="Activated sludge of municipal
                     wastewater treatment plants"
                     /db_xref="taxon:2737536"
                     /environmental_sample
                     /geo_loc_name="Canada"
                     /lat_lon="49.25 N 123.1 W"
                     /collection_date="2015-06-16"
                     /metagenome_source="wastewater metagenome"
                     /note="metagenomic"
     gene            <1..2032
                     /locus_tag="PLF56_05605"
     CDS             <1..2032
                     /locus_tag="PLF56_05605"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_020388682.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=2
                     /transl_table=11
                     /product="copper-translocating P-type ATPase"
                     /protein_id="HOQ53072.1"
                     /translation="IPVEKLIAVVEQAGYGASLPDPAKPEVDHAAQLRSRVVISAVLA
                     VPVIALAMVPAWQFAGWQWVSLALTVPIYAWAAWPFHRSALVNARHGATTMDTLISLG
                     TTAAFGWSLYALFFGHAGMIGYTHPFEFMLMRGSGEASIYFEAVAAIVTFLLLGRYFE
                     ARSKQKAGEAVRALLQLGATEATLLRGGVATRVPVDFLKAGDVFVVRPGEKIATDGEV
                     VDGSSAVDVSMVTGESVPVDVAVGDAVIGATLNANGRLVVRATRVGADTQLAHIARLV
                     EQAQTGKANVQRLADRISAVFVPIVIGLAVLTWLGWLLAGAGLAFASSAAVAVLIIAC
                     PCALGLATPTALLVGTGRGAQLGIVIKGPEILERTKSVGVIVLDKTGTVTTGEMQVVS
                     VVGADPSDEAWLRFAGAAEGASEHPIARAIAAHVDSDAVVEAFENVPGQGVRATIEGH
                     NVLVGTPALVAEAAPDASVAAAFAAAQGRGHTAVLVAVDGVLRGMIAVADAVKPTSPE
                     AIAQLKALGLAPVLLTGDSRMVAESVGEQVGIERVIAEVLPSDKLAEVRRLQATGTQV
                     AMVGDGVNDAAALAQADLGIAMGTGTDAAIAASDLTLMRGDLRLAATAIRLSRATLAT
                     IKGNLFWAFAYNVAAIPLAASGYLNPMIAGAAMAFSSVFVVLNSLRLRTFRS"
     gene            complement(2250..3659)
                     /locus_tag="PLF56_05610"
     CDS             complement(2250..3659)
                     /locus_tag="PLF56_05610"
                     /EC_number="2.4.-.-"
                     /inference="COORDINATES: protein motif:HMM:NF024831.2"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="glycosyltransferase"
                     /protein_id="HOQ53073.1"
                     /translation="MVDQAPDLPAPSPDDPGTFEFPRTEEPRSTADASGRPLTIAQFT
                     DNYGPAHSGLLYAVQFLESEVLAAGHRVLLVAPEADGPNPLAGDPHRREIRMPSVPIP
                     GAGIRLSLGKDLEFRLAQLIADPPDIIHVHGLGGVGLLGVWVAQRANRPLVVTWHTDL
                     EAYAEHYWHLGPFLYAAYQMFALRAEPKPWAKLAKLRVKRPRRGGAQLQLLQAAADML
                     TSADVVTTPSDKTGRRVLELAPGAHVRVVPNGLDPLPPRAPIPPAKGPRLLYVGRVSA
                     EKGLDLLLDAFELVQDFEPDAELMIVGDWHEGPPGLRRRLQKAARRGGTRLVGRVPRE
                     ELAAYYSGSDIFVFPSLTDTQALVLHEAALCGMPLVITDPELNLVVDPGVNALVARPN
                     MVSLSQAIMSMLRQLKDPAFASRAASRSRELGAEWTIDRQATEMMSIYGDLIAGRPVP
                     VSEGLAPDLGRPRLGGRLH"
     gene            complement(3985..5004)
                     /locus_tag="PLF56_05615"
     CDS             complement(3985..5004)
                     /locus_tag="PLF56_05615"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_014845845.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="dihydroorotate dehydrogenase-like protein"
                     /protein_id="HOQ53074.1"
                     /translation="MNLGTRYLGLNLRNPVVASAGPLSQTVDGVRALASAGVGAVVMY
                     SLFEEQVRRELERDAALEEAHEESFAEALSYFPAVATPTERGVADTYVDLVRRAAAAV
                     DVPVIASINGASLGGWVEIAQQLADAGASAIELNIYFVPGNLTMTGAEVEQRHLDIVA
                     AVRQAVALPIAVKLSPFFSSPGNMALRLIVAGADGLVLFNRFLQPDVDIETLSVKPSV
                     ALSNRFEGRLPRTWIATLSEHTSASLAATSGVEESADVVRYLLAGADVVMSTSALVRH
                     GAGYAANLIAGLQDWMTRHGYSSVDDFRGLLAVPADADANALQRAGYVAALENARRTY
                     GTLHR"
     gene            complement(5027..>6204)
                     /locus_tag="PLF56_05620"
     CDS             complement(5027..>6204)
                     /locus_tag="PLF56_05620"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_014845844.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_function: GO:0003824 - catalytic activity [Evidence
                     IEA];
                     GO_function: GO:0030976 - thiamine pyrophosphate binding
                     [Evidence IEA]"
                     /codon_start=3
                     /transl_table=11
                     /product="thiamine pyrophosphate-dependent enzyme"
                     /protein_id="HOQ53075.1"
                     /translation="SIYGGNLPTTPWSRNKYGRGPAWSNSLFEDNAEFGLGLRLAADL
                     QTDLARQRLDALRPELSDELVELILIAPQQYESDLSHQRARVEHVKVLLDEMLTTRST
                     PSRVSVLFPLGIAGPEGGEAELTGRRGQPVAAEVEAIPALPPGSPLAEGRLRLVEDLR
                     SVADHLLRRSVWIVGGDGWAYDIGSAGVDHVLASGRDINVLVLDTEVYSNTGGQSSKS
                     TPMGAVAKFAAAGKTTYKKDLAMQAIAYGNVYVARVAMGADPQQTLRAFREAEMYDGP
                     SLILAYSHCIAHGIDMRKGLDQQYRAVNSGHWPLMRFNPVLREADKNPFLLDSPRPRV
                     SLRDYHKNELRFRMLTNTHPEEAERLLQLGQEQVLRRFAEYEQMASRSANMFAASPR"
BASE COUNT          910 a         2118 c         2194 g          982 t
ORIGIN      
        1 gatcccggtc gagaaactga tcgccgtcgt cgagcaggcc gggtatggcg cgtccctgcc
       61 cgacccggcc aaacccgagg tcgaccacgc cgcccagctg cgctcacggg tggtgatcag
      121 cgctgtgctg gcggtgccgg tgatcgcgct ggccatggtg ccggcctggc agttcgcggg
      181 ctggcagtgg gtctcgctcg ccctgaccgt gccgatctac gcgtgggcgg cctggccgtt
      241 ccaccgctcg gcgctggtca acgcccggca cggcgccacc accatggaca ccctgatcag
      301 cctcggcacc accgccgcct tcggctggtc gctgtatgcg ctgttcttcg ggcacgccgg
      361 catgatcggc tacacgcatc cgttcgagtt catgctgatg cggggcagcg gcgaggccag
      421 catctacttc gaagcggtcg ccgccatcgt cacgtttctg ctcctgggac gctatttcga
      481 ggcgcgctcg aagcagaagg cgggcgaggc cgtccgggcg ctgctgcagt tgggcgccac
      541 cgaggcgacg ctgctgcgcg gaggcgtcgc aacccgggtt ccggtcgact ttctcaaggc
      601 cggggacgtg ttcgtggtcc gtccgggcga gaagatcgcc accgacggcg aggtcgtcga
      661 cggcagttcg gccgtggacg tctcgatggt cacgggcgaa tccgtgccgg tggacgtggc
      721 ggtcggcgac gccgtgatcg gggccaccct caacgccaac ggacggctcg tcgttcgggc
      781 aaccagggtc ggtgccgaca cccaactggc gcacatcgcg cggctggtcg agcaggccca
      841 gaccggcaag gcgaacgtgc agcggctcgc ggaccggatc agcgccgtct tcgtgccgat
      901 cgtgatcggg ctggcggtgc tcacctggct cggctggctg ctggcggggg ccgggctggc
      961 gttcgcgtcg tcggcggctg ttgcggtgct gatcatcgcc tgcccgtgcg cactggggct
     1021 ggccacaccg accgcgctgc tggtcggtac cggacggggc gcgcagctcg gcatcgtgat
     1081 caagggtccc gagattctgg agcgcacgaa gtccgtcggc gtcatcgtgc tggacaagac
     1141 cggcaccgtg acgaccggcg agatgcaggt ggtgtcggtg gtgggcgccg acccgtcgga
     1201 tgaggcctgg ctgcggttcg cgggggcggc ggaaggggcg tccgagcatc cgatcgcgcg
     1261 ggcgatcgcg gcgcacgtgg actccgatgc ggtggtggag gccttcgaga acgtgccggg
     1321 gcagggcgtc cgagcgacga tcgaggggca caatgtgctg gtcggcactc cggcgctggt
     1381 cgctgaggcg gctcctgacg cctcggtggc cgcagcgttc gccgcggctc agggcagggg
     1441 ccacacggcc gtcctggtgg ccgtggacgg cgtcctgcgc ggcatgatcg ccgtcgccga
     1501 cgcggtgaaa ccgacctcgc cggaggcaat cgcccaactc aaggctctcg gtctcgcgcc
     1561 cgtgctgctg accggtgaca gccgcatggt ggctgaatcc gtgggcgaac aggtgggcat
     1621 cgagcgggtg atcgccgagg tgctgccctc ggacaagctg gccgaggttc ggcggctgca
     1681 ggccaccggc acgcaggtgg cgatggtcgg cgacggggtc aacgacgcgg ccgcgctggc
     1741 ccaggccgat ctgggcattg cgatgggcac cggcaccgac gcggcgatcg cggcgtccga
     1801 cctcacgctg atgcggggcg atctgcgcct ggcggcaacg gccatcaggc tgtcgcgggc
     1861 caccctggcc accatcaagg gcaacctgtt ctgggccttc gcctacaacg tggcggcgat
     1921 tccactggct gcgtcggggt atctcaaccc gatgatcgcc ggggccgcga tggcgttctc
     1981 gtcggtgttc gtggtgctga acagcctgcg cttgcgaacg ttccgcagct gagcgtgggg
     2041 cgagccgcct gccagcctgt tgacgcggcc tgacccaggc agattctgcg ggcatccgct
     2101 cgctcggccg ggagttcatc gtctgggggt ataccgattt taccgccaga ttcgaagaat
     2161 cgccctctgg aacgtttcat cacgaatctg gcggtagatt tggtatatcg gtacctggtg
     2221 agattctgct acagggccga tgggcgttct cagtgcagcc gcccgccgag ccgggggcga
     2281 ccgagatcgg gggcgaggcc ctcgctgacc ggcaccgggc gtccggcgat caggtcgccg
     2341 tagatgctca tcatctcggt cgcctggcga tcgatcgtcc attcggcacc gagttcacgg
     2401 ctgcgggagg cggcgcgcga agcgaaggcc ggatccttga gttgccgcag catcgacatg
     2461 atggcctggc tcagtgaaac catgttcggc cttgccacta gggcattcac ccccggatcg
     2521 acaaccaggt tcagctccgg atcggtgatc accagcggca taccgcacag tgcggcctcg
     2581 tgcagcacca gcgcctgggt gtcggtcaac gaggggaaga cgaagatgtc cgatccgctg
     2641 tagtaggcgg ccagttcctc gcgcggcacc cgtccgacca gccgcgtacc gccgcgccgg
     2701 gccgccttct gcagccgtcg gcgcaacccg ggcggtccct cgtgccagtc gccgacgatc
     2761 atcagctcgg cgtccggctc gaagtcctga acgagctcga acgcgtccaa cagcaggtcg
     2821 aggcctttct cggccgagac ccgtccgaca tagagcagcc ggggaccctt ggcaggcggg
     2881 atcggtgccc gcggcggcag cgggtcgagc ccgttgggca ccacgcgcac gtgcgcgccg
     2941 ggagcgagct cgagcacccg gcgtccggtc ttgtcggagg gtgtggtgac cacgtcggcg
     3001 ctggtcagca tgtcggcggc ggcctgcagc aactgcaatt gggcgccgcc gcggcgcgga
     3061 cgcttcaccc gcagcttggc cagcttcgcc cagggtttgg gctctgcccg cagcgcgaac
     3121 atctggtagg cggcgtagag gaacgggccg aggtgccagt agtgctcggc gtaggcctcc
     3181 aggtcggtgt gccaggtgac caccagcgga cggttcgcac gctgcgccac ccacacgccc
     3241 agcaggccga cgccgccgag gccgtgcacg tggatgatgt cgggtgggtc ggcgatcagc
     3301 tgggcaaggc gaaactccag gtcctttccg agcgacagcc gaataccggc acccggaatc
     3361 ggcaccgacg gcattcggat ctcgcggcgg tgcgggtcgc cggccagggg gttggggccg
     3421 tccgcctcgg gggcgaccag cagcactcgg tgcccggcgg ccagcacctc ggactccaga
     3481 aactgaaccg cgtacagcag gcccgagtgc gccggaccgt agttgtcggt gaactgcgcg
     3541 atcgtgagcg gacgccccga cgcgtcggcg gtcgaccggg gctcttcggt gcgcggaaac
     3601 tcgaaggtgc cggggtcgtc cggactcggg gcggggaggt ccggtgcctg atcaaccatg
     3661 ggttcaggct aatggctcgc tcaccgctca ccgctcggtg cgaggggcaa tgcgaccgac
     3721 gattctgaaa tcacccgcac ccgtggtgca ggacctcggc gtgtcgggca gcgagcccgc
     3781 ggctggtgtc ctgatctcag agaatcctgc tagggcgtgt ctcctcaccc tgagcgttcc
     3841 gcgaggacgt gcccagtgcg tctgatcaca aggcggaaga cgagtcgata gcgggtcctc
     3901 tcgccgagga tgacaacgca gtgagcaggc gtactgggtg cgccgcagcg cggccagggt
     3961 gaggagaccc gccctaccgc ttcctcagcg gtgcagggtg ccgtaggtgc gccgggcatt
     4021 ctccaatgcc gccacgtagc cggcgcgctg caatgcgttc gcgtcggcgt ccgccggtac
     4081 cgccaacagg ccgcggaagt cgtcgaccga tgagtagccg tgccgggtca tccagtcctg
     4141 cagacccgcg atcaggttgg ccgcgtaccc ggcgccgtgg cgcaccagcg ccgatgtgct
     4201 catcaccacg tcggcgccgg ccagcaggta gcgcaccacg tccgcggact cctccacccc
     4261 tgaggtcgcc gccagcgacg cgctggtgtg ctccgacagg gtggcgatcc aggtgcgcgg
     4321 cagccgtccc tcgaaccggt tcgacaaggc cacggacggc ttgacgctga gcgtctcgat
     4381 gtcgacgtcg ggttgcagaa accggttgaa cagcaccagc ccgtcggcgc cggccacgat
     4441 cagccgcagc gccatgttgc ccggcgagga gaagaacggg ctcagcttca ccgcgatcgg
     4501 cagtgcgacc gcctgccgca ccgcggccac gatgtcgagg tgtcgttgct cgacctcggc
     4561 gcccgtcatg gtcaggttgc ccggtacgaa atagatgttc agctcgatcg cggacgcccc
     4621 cgcgtccgcc agttgctgcg cgatctcgac ccagccgccc aggctggcgc cgttgatgct
     4681 ggcgatcacc ggcacgtcca cggcggcggc ggcccgtcgg accaggtcga cgtaggtgtc
     4741 ggccacgccc cgttccgtcg gcgtcgcgac cgccggaaag tagctcagcg cctcggcgaa
     4801 cgactcttcg tgcgcctctt cgagcgcggc gtcgcgctcc agttcgcggc gcacctgctc
     4861 ctcgaacagg gagtacatca ccaccgcacc cacaccggcc gacgccagcg cgcggacccc
     4921 gtccacggtc tgtgacaggg ggccggccga ggccaccacc gggttgcgca ggttcagccc
     4981 cagatagcgg gtgcccaggt tcatcgttcg ccccttctcg gcggtgtcag cgtggggacg
     5041 cggcgaacat gttcgcgctg cgtgaggcca tctgctcgta ctcggcgaac cggcgcagca
     5101 cctgctcctg acccagttgc agcaggcgtt cggcctcttc ggggtgcgtg ttggtcagca
     5161 tccggaaccg caactcgttc ttgtgatagt cgcgcagcga cacccgcgga cgcggcgagt
     5221 cgagcaggaa cgggttcttg tcggcctcgc gcagcaccgg gttgaaccgc atcagcggcc
     5281 agtgccccga gttgaccgcc cggtactgct ggtcgagccc cttgcgcatg tcgatgccgt
     5341 gcgcgatgca gtggctgtac gccaggatca gggacggtcc gtcgtacatc tcggcctcgc
     5401 ggaacgcccg caacgtctgc tgcgggtcgg cccccatcgc cacccgggcc acgtacacgt
     5461 tgccgtaggc gatcgcctgc atcgccaggt ccttcttgta ggtggtcttg ccggccgccg
     5521 cgaacttggc caccgccccc atcggcgtcg atttggacga ctgcccgccg gtgttgctgt
     5581 acacctcggt gtcgagcacc agcacgttga tgtcgcgccc ggacgccagc acgtggtcga
     5641 cccccgccga cccgatgtca taggcccagc cgtcgccacc gacgatccac accgagcgac
     5701 gcagcaggtg atccgcgacg ctgcgcagat cctcgaccag ccgcaaccgg ccctcggcca
     5761 ggggtgagcc cggcggcagc gccgggatcg cctcgacctc ggcggcgacc ggctggccgc
     5821 gccgtccggt cagctccgcc tcgccacctt cggggccggc gatccccaac gggaacagca
     5881 cgctcacgcg tgacggcgtc gaacgagtgg tcaacatctc gtcgagcagc accttgacgt
     5941 gctcgacccg cgcgcgctga tgcgacaggt ccgactcgta ctgctgcgga gcgatcagga
     6001 tcagctcgac cagctcgtcg ctcaactcgg gacgcagcgc gtccagccgt tgccgggcca
     6061 ggtcggtctg caggtcggcc gccagccgca gccccagccc gaactctgcg ttgtcctcga
     6121 acaacgagtt cgaccaggcc gggccgcgtc cgtacttgtt gcgcgaccag ggcgtggtgg
     6181 gcaggttgcc gccgtagatg gacg
//
LOCUS       DAOSUG010000128         6194 bp    DNA     linear   ENV 10-JUN-2024
DEFINITION  MAG TPA_asm: Micropruina sp. isolate AMR_MDS_2195
            NODE_5923_length_6194_cov_4.523701, whole genome shotgun sequence.
ACCESSION   DAOSUG010000128 DAOSUG010000000
VERSION     DAOSUG010000128.1
DBLINK      BioProject: PRJNA925477
            BioSample: SAMN32864262
            Sequence Read Archive: SRR4236673
KEYWORDS    WGS; ENV; Metagenome Assembled Genome; MAG; Third Party Data; TPA;
            TPA:assembly.
SOURCE      Micropruina sp. (wastewater metagenome)
  ORGANISM  Micropruina sp.
            Bacteria; Bacillati; Actinomycetota; Actinomycetes;
            Propionibacteriales; Nocardioidaceae; Micropruina.
REFERENCE   1  (bases 1 to 6194)
  AUTHORS   Abdulkadir,N., Saraiva,J.P., Zhang,J., Stolte,S., Gillor,O.,
            Harms,H. and Rocha,U.
  TITLE     Genome-centric analyses of 165 metagenomes show that mobile genetic
            elements are crucial for the transmission of antimicrobial
            resistance genes to pathogens in activated sludge and wastewater
  JOURNAL   Microbiol Spectr 12 (3), e0291823 (2024)
   PUBMED   38289113
REFERENCE   2  (bases 1 to 6194)
  AUTHORS   Abdulkadir,N.
  TITLE     Direct Submission
  JOURNAL   Submitted (24-MAR-2023) Environmental Microbiology, Helmholtz
            Center for Environmental Research -UFZ, Permoserstr 15, Leipzig
            04318, Germany
COMMENT     The annotation was added by the NCBI Prokaryotic Genome Annotation
            Pipeline (PGAP). Information about PGAP can be found here:
            https://www.ncbi.nlm.nih.gov/genome/annotation_prok/
            
            ##Genome-Assembly-Data-START##
            Assembly Method        :: SPAdes v. 3.11.1
            Genome Representation  :: Full
            Expected Final Version :: Yes
            Genome Coverage        :: Not Applicable
            Sequencing Technology  :: Illumina MiSeq
            ##Genome-Assembly-Data-END##
            
            ##Genome-Annotation-Data-START##
            Annotation Provider               :: NCBI
            Annotation Date                   :: 05/04/2023 04:57:10
            Annotation Pipeline               :: NCBI Prokaryotic Genome
                                                 Annotation Pipeline (PGAP)
            Annotation Method                 :: Best-placed reference protein
                                                 set; GeneMarkS-2+
            Annotation Software revision      :: 6.5
            Features Annotated                :: Gene; CDS; rRNA; tRNA; ncRNA
            Genes (total)                     :: 2,776
            CDSs (total)                      :: 2,750
            Genes (coding)                    :: 2,714
            CDSs (with protein)               :: 2,714
            Genes (RNA)                       :: 26
            tRNAs                             :: 24
            ncRNAs                            :: 2
            Pseudo Genes (total)              :: 36
            CDSs (without protein)            :: 36
            Pseudo Genes (ambiguous residues) :: 0 of 36
            Pseudo Genes (frameshifted)       :: 2 of 36
            Pseudo Genes (incomplete)         :: 32 of 36
            Pseudo Genes (internal stop)      :: 2 of 36
            ##Genome-Annotation-Data-END##
FEATURES             Location/Qualifiers
     source          1..6194
                     /organism="Micropruina sp."
                     /mol_type="genomic DNA"
                     /submitter_seqid="NODE_5923_length_6194_cov_4.523701"
                     /isolate="AMR_MDS_2195"
                     /isolation_source="Activated sludge of municipal
                     wastewater treatment plants"
                     /db_xref="taxon:2737536"
                     /environmental_sample
                     /geo_loc_name="Canada"
                     /lat_lon="49.25 N 123.1 W"
                     /collection_date="2015-06-16"
                     /metagenome_source="wastewater metagenome"
                     /note="metagenomic"
     gene            complement(<1..184)
                     /locus_tag="PLF56_05625"
     CDS             complement(<1..184)
                     /locus_tag="PLF56_05625"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_020576846.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="biotin--[acetyl-CoA-carboxylase] ligase"
                     /protein_id="HOQ53076.1"
                     /translation="MTLEPLVDAAALTAALVGPGRPVTSVRSVAATGSTNADLAAAAA
                     SGAPAGTVLVADHQCAG"
     gene            444..1241
                     /locus_tag="PLF56_05630"
     CDS             444..1241
                     /locus_tag="PLF56_05630"
                     /inference="COORDINATES: protein motif:HMM:NF017853.2"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="DUF937 domain-containing protein"
                     /protein_id="HOQ53077.1"
                     /translation="MSAADDIIADLPLDQLAQQLGTDPATAEAAVRAALPSLFGGLQA
                     NAQTGEQSSLSLAEALTQHSDPQFLEGGVNLDDVDTDDGHAIVQHIFANSPEQAQVLQ
                     ASSAGAGGNLINKVLPILAPIVMAYLAKRLSVGSSSQQRQSSESASGGMGGLGDLLGP
                     ILGGLFGGTASAQSGAAGGGLGDILGQIFGDGGQQAPAQQAPAQQAPAEKHDSGQWTQ
                     PQFDQGGDSNGPLSMPSVQEDPRDSGSQQQQPQSGGIGDILGGLFGR"
     gene            1238..1840
                     /locus_tag="PLF56_05635"
     CDS             1238..1840
                     /locus_tag="PLF56_05635"
                     /EC_number="3.6.1.-"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_013862246.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="Maf family protein"
                     /protein_id="HOQ53078.1"
                     /translation="MRVVLASASPARLEVLRRAGVSAEAIVSGVDEAAIEVDPPAALA
                     LTLARLKAAAVADDLSGDVPTVVIGCDSLLDFEGQAQGKPAGVAEARQRWQAMRGRSG
                     ALHTGHRVIVIDSDGSRATEAVASTVVHFADLSDGEIDAYVATGEPLLVAGAFTVDGL
                     GGPFVTGIEGDHHNVVGLSLPLLRSMLLELGVAWPSLWEA"
     gene            1867..2772
                     /locus_tag="PLF56_05640"
     CDS             1867..2772
                     /locus_tag="PLF56_05640"
                     /EC_number="2.4.2.45"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_013862236.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_function: GO:0016765 - transferase activity,
                     transferring alkyl or aryl (other than methyl) groups
                     [Evidence IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="decaprenyl-phosphate phosphoribosyltransferase"
                     /protein_id="HOQ53079.1"
                     /translation="MSEHTLVQPTKSRLPAWLRAMRPKQWVKNILVFAAPVAAGALFV
                     PQVFVNTLWAFVAFSLISASIYLINDVRDVDADRQHPKKRFRPIAAGEIAARPATVLA
                     GVTMIASLAIGWWVAPMLGITLAVYWLLQVGYSMFLKHQPILDLAMVAAGFLLRAVAG
                     GVASGIELSQWFLLVASFGSLFMVAGKRYSEMKELGSEAGTRASLERYSLSYLRFVWA
                     ISCSIVIMSYSLWTFEQAREGQLWGVPWTTISIVPFTLALLRYAMDIDAGNAGEPEDV
                     VLNDRVLQGLAVLWLIPLGVGIFLH"
     gene            2914..3612
                     /locus_tag="PLF56_05645"
     CDS             2914..3612
                     /locus_tag="PLF56_05645"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_013162078.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_function: GO:0016491 - oxidoreductase activity
                     [Evidence IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="SDR family oxidoreductase"
                     /protein_id="HOQ53080.1"
                     /translation="MFVMKTALITGGSRGIGRAIAEALGPHHHLLIGGRDPAAVGAVV
                     GALPSAEPFVADLTDESTLATAVAGIDSLDVLVHSAGGSPLGSIAETDPQVWRDVLEV
                     NLIAPANLTRLLLPALRRARGHVVFINSGAGFTARAGWGAYAASKFGLRALADSLRTE
                     EEGVVKVTSIHPGRTATDMQRSIREHEGEPFVPGDFLAPAAVAATVRLALDSPANASI
                     DTLTIRPVGPPRME"
     gene            complement(3795..4727)
                     /locus_tag="PLF56_05650"
     CDS             complement(3795..4727)
                     /locus_tag="PLF56_05650"
                     /inference="COORDINATES: ab initio
                     prediction:GeneMarkS-2+"
                     /note="Derived by automated computational analysis using
                     gene prediction method: GeneMarkS-2+."
                     /codon_start=1
                     /transl_table=11
                     /product="hypothetical protein"
                     /protein_id="HOQ53081.1"
                     /translation="MKVRTPLSEDMIALLDAQCGVLSRDQLLRLGVSDRVIHRLLGGG
                     WLNVLERGIYLRGGSLSWLGRAWAGVLLGGDDAVLGGASAAFLHGLEKTEPSTIEVFT
                     PRQRGPLTGYVFSRAVRQGVGEPPRTTYEATVLDLCADRDEDELAALLADAVSGRRTK
                     PKSLLAEIERRTRVPNRALLCQMLGQVAGGTHSALERRYLVDVETPHGLPTATRQAGP
                     HAAHRGDAWYQQFGVMVELDSKLHHSGGAAFRDLNRDNDHALMGIITLRFGWADVTGI
                     AVCHTAQVVADALTARGWAGPLSPCRRCARVHDI"
     gene            complement(5632..>6194)
                     /locus_tag="PLF56_05655"
     CDS             complement(5632..>6194)
                     /locus_tag="PLF56_05655"
                     /inference="COORDINATES: protein motif:HMM:NF013694.2"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_function: GO:0046873 - metal ion transmembrane
                     transporter activity [Evidence IEA];
                     GO_process: GO:0030001 - metal ion transport [Evidence
                     IEA]"
                     /codon_start=3
                     /transl_table=11
                     /product="magnesium transporter CorA family protein"
                     /protein_id="HOQ53082.1"
                     /translation="VHGLLDWLVDGHFEAVQVMDDKLEDLEDLLFEDAVRTREVQQRT
                     FALRKALVRLRRVVLPMREVVAGVMRHRLSGEWQRSPELDGEFNDLYDHVLRAAEWTE
                     SLRDMVASVFETNLSLQDARLNTIMKKLASWGAIIAVPTAVTGWFGQNLPYWGFSSAW
                     GLWQSVLLIVVGTVGLYWLFRRNDWL"
BASE COUNT         1015 a         2200 c         2021 g          958 t
ORIGIN      
        1 gtccggcaca ttgatggtcg gccaccagca ccgtacccgc gggtgcaccg gacgccgcgg
       61 ccgccgccag gtcggcattg gtcgaaccgg tcgcagccac gctgcggacc gaggtcaccg
      121 gacgacccgg cccgaccagt gcggcggtca gtgcggctgc atcgacaagc ggctcgagag
      181 tcatagcggc agcctaagct aggcctggat ccaccgatct tcgcctactg cgcggcccca
      241 tccgcgccct ggctgcgtcg gccacgctca ccagacgccc ggcgtgccat cgcgcggacc
      301 ggctcgccag gccacccttc tggtgccgct cgcgacggcg ccccatcggt ggatccaggc
      361 taaacaccca ttccctcaac agggccggag cggcagaatg ggcaccgatc tgtcaccgat
      421 caccgacagc aagggaacct gccatgagcg ccgccgacga catcattgcc gacctgccac
      481 tggatcaatt ggcccagcag cttggcaccg acccggcaac cgcagaagcc gccgtgcgag
      541 ccgccctgcc gtcgctgttc ggaggcctgc aggccaacgc gcaaaccggc gagcagagct
      601 cgctcagcct cgccgaggcg ctgacacagc acagcgatcc tcagtttctg gaaggcggcg
      661 tcaacctcga tgacgtcgac accgacgacg gacacgccat cgtgcagcac atcttcgcca
      721 actcacccga acaggcacaa gtactgcagg ccagctccgc gggcgctggt ggcaacctga
      781 tcaataaggt cttgccgatc ctggccccca tcgtgatggc ctacctggcc aagcgcctga
      841 gcgtgggttc gtcgtcgcag cagcgtcagt cgtccgagag tgcctccggt ggtatgggtg
      901 gcctgggtga tctgctcggg ccgattctgg gcggcctctt cggcggcacc gcgtccgcac
      961 agtctggggc cgccggcggg ggtctcggcg acattctggg ccagatcttc ggcgacggtg
     1021 ggcagcaggc gccggcccag caggccccgg cccagcaggc cccggccgaa aagcacgaca
     1081 gcggccagtg gacccagccg cagttcgatc agggcggtga ttcgaacggc ccgctgagca
     1141 tgccgagcgt gcaggaggat ccccgcgatt ccggctcgca gcaacagcag ccgcaatccg
     1201 ggggcatcgg cgacatactg ggcggcttgt tcggccgatg agggtcgttc tcgcctcggc
     1261 cagcccggcc cgcctcgagg tgctgcggcg ggccggggtt agcgccgagg ccatcgtctc
     1321 gggtgtcgac gaggcagcga tcgaggtcga cccgcccgcg gcgctggccc tcacgctcgc
     1381 ccgcctgaag gccgcggcgg tcgctgatga cctcagcggt gacgtgccga cggtcgtgat
     1441 cggctgcgac tcactactgg actttgaggg gcaggcgcag ggaaagccgg ccggggttgc
     1501 tgaggcccgg cagcgctggc aggcgatgcg cgggcgctcg ggcgccctgc acaccggtca
     1561 ccgggtgatc gtgatcgact cggacggttc gcgagccacc gaggcggtgg cgtccaccgt
     1621 ggtccatttc gccgatctca gcgacggtga gatcgacgcc tacgtcgcga ccggcgaacc
     1681 gctgttggtg gccggcgcgt tcaccgtgga cggcctgggc ggccccttcg tcaccgggat
     1741 cgagggcgat caccacaacg tggtcgggct gtccctgcca ttgctgcgca gcatgctgct
     1801 tgagctcggc gtggcctggc cgagcctctg ggaggcgtag ggcccgctgc gatagcctgt
     1861 cgctcgatgt cggaacacac gctggtacag cccaccaagt cccgcctgcc cgcgtggctg
     1921 cgggcgatgc gtcccaagca gtgggtgaag aacattctgg tgtttgcggc gcccgttgcc
     1981 gccggcgccc tgttcgtccc gcaggtgttc gtcaacaccc tctgggcgtt cgtcgcgttc
     2041 tcgctgatca gcgcatccat ctatctgatc aacgacgtcc gcgacgtgga cgcggaccgg
     2101 cagcacccga agaagcggtt ccgtccgatc gcggccggtg aaatcgccgc caggccggcc
     2161 acggtgctgg ccggggtgac catgatcgcc agcctggcga tcggctggtg ggtggcaccg
     2221 atgctcggca tcacgctggc ggtctactgg ctgcttcagg tcggctattc gatgtttctc
     2281 aagcatcagc cgatcctcga cctagcgatg gtcgcggccg gcttcctgct gcgcgcggtg
     2341 gccggcggcg tggcgtccgg catcgagttg tcgcagtggt tcctgctggt ggcctcgttc
     2401 ggctcgctgt tcatggtggc cggcaagcgc tactcggaga tgaaagagct gggctcggag
     2461 gcaggcacac gggcctcact cgagcggtat tcgctcagct acctgcgttt cgtctgggcg
     2521 atctcgtgct cgatcgtgat catgtcgtac agcttgtgga ctttcgagca ggcccgcgag
     2581 gggcaactgt ggggcgtgcc gtggaccacg atctcgatcg tgccgttcac cctggcgctg
     2641 ctgcgctacg ccatggacat cgacgccggc aacgccggcg agcccgaaga cgtggtgctc
     2701 aacgaccgag tgctgcaggg gctggccgtg ctctggctga ttccgcttgg cgtcggcatc
     2761 tttctgcact gaggattcgg cggctgctcg ggactcagcg gttcgttcga ccggcgcccc
     2821 accggacgtg gcccgcccca acccagcgtt gccccgcgtt ccaacggtgg caacgtcagc
     2881 catggcaggc aacgtgggtt cgactcagcc agaatgttcg tcatgaagac cgcactgatc
     2941 accggaggct cgcgcggtat cggccgcgcc atcgccgagg cactcggccc ccaccaccac
     3001 ctgttgatcg gtggtcgtga cccggccgca gtgggggccg tcgtcggagc actgccgagc
     3061 gctgagccgt tcgtcgccga cctcaccgac gagagcacgc tggccacggc ggtggcgggc
     3121 atcgactccc tcgacgtgct ggtgcactcg gccggcggct caccgctggg atccatcgcc
     3181 gagactgacc cgcaggtatg gcgcgacgta ctcgaggtga acctgatcgc acccgcgaac
     3241 ctgacccgtc tgttgcttcc cgcgctgcgc cgggcccgcg gtcacgtggt gttcatcaac
     3301 tccggtgccg gcttcaccgc acgggccggc tggggcgcct acgccgcatc gaagttcggc
     3361 ctgcgggcgc tggccgattc gctgcgaacc gaggaagaag gggtggtgaa ggtcacctcc
     3421 atccatcccg gccgcaccgc gaccgacatg cagcgctcga tccgcgaaca cgagggggaa
     3481 ccgttcgttc cgggcgactt cctggcgccg gccgcagtgg ccgcgaccgt gcgcctggcc
     3541 ctggactccc ccgcgaacgc ctccatcgac accctgacca tccggcccgt tggccctccg
     3601 aggatggagt gaatccgccc accgaactcg gtacacggtt tcactccttg ttcacggggt
     3661 cgcgctcggg ggtgccactg acgtcggtcg atcgttccgg aagcagagcg cacctcgctg
     3721 ggttccccac ggtcgagtcg acgcatggct acttcgtcac cccagcgacg cacgcagacg
     3781 cgcgacccga cgcctcaaat gtcgtgcacc cgtgcgcacc ggcggcacgg cgagagcggt
     3841 cccgcccagc cgcgggcggt caaggcgtcg gcgacgacct gcgcggtgtg gcacacggcg
     3901 ataccggtga catccgccca gccgaagcgc agcgtgatga tgcccatcag tgcgtgatcg
     3961 ttgtcgcgat tcaggtcgcg aaaggcagcg cctccgctgt gatgcagctt gctgtccagt
     4021 tcaaccatca caccgaactg ctgataccag gcgtcacctc ggtgggcagc gtgcggaccg
     4081 gcctgcctgg tcgccgtcgg cagcccgtgg ggcgtctcga catcgacgag atagcgacgc
     4141 tccagcgccg aatgggtgcc gccggcgacc tgccccagca tctggcacag caacgcacgg
     4201 ttgggcaccc gggtgcgccg ctcgatctca gccagcaggc tcttgggttt ggttcgccgc
     4261 ccactcaccg catcggcgag cagtgcggcc aactcgtcct cgtcgcggtc agcgcagaga
     4321 tcgagcacag tggcctcgta cgtcgtccgg ggcggttcgc ccaccccctg ccgaacagcc
     4381 cgcgagaaca cataaccggt cagcggcccg cgctggcgag gcgtgaacac ctcgatggtc
     4441 gaaggttcgg tcttctccag cccgtgcagg aaggccgccg aggcgccacc cagcacggcg
     4501 tcgtcaccac ccagcagcac acccgcccag gcccggccca gccatgacaa cgagcctccg
     4561 cgcaggtaga tcccgcgctc gagaacgttc agccacccgc cgcccagcaa ccggtggatc
     4621 acccggtcgg acaccccgag ccgcagcagc tggtcgcgcg acagcacgcc gcactgggcg
     4681 tcgagcagcg cgatcatgtc ctcgctgagc ggagttcgca ccttcacaca agcagtctga
     4741 gcacctgcgc gcgcaagtgt tcacaatcgt cgatgcgctc gagcctgttc agacgtgatc
     4801 catcgctggt cgagctgtcg agattttccc gagcaggggt ctcgccgagc tcagccaaga
     4861 tgacgaagtg gatccaccta ccaaactcgg tacaccagtt cactgcatcc tgaggggcag
     4921 cggcgtaccg gacggcggac aacatcctga gggcagcggt gcgtaccgga cggcggacaa
     4981 catcctgagg gcagcggtac gtaccggccg gcggacaacg cccttcccgg tccaaccccg
     5041 tgagggcgcc gccgccggtc gcctgcacgc caggggtgtc ggcgctgatc cggcgcatcg
     5101 gtatcggcgg ggccactacg cgtcgaggat ccgaacagca gccacgcgac accgctcacg
     5161 tgtcatccgg cacggcggtg acctaccaga acgagagagg cgaggtgacc gacgccggcg
     5221 aggtctacct cgacccgaac gccaacgcgg gcaccaccgt ggagggtagc cccgccgcta
     5281 cggtgaatga tcgtcgcgca agcgaggcaa cccaacgacc aacccaaccg ctgacgcgga
     5341 ttcgcggaga ctttgcccca ccacccgccg cgaagccttg ccccaccacc tgccgcgaag
     5401 ccttgccgca ccacctgccg cgaagccgcg gccggttgct gatcagggaa cccgggatcg
     5461 atacgcgaac cgttggttga gctttcgaaa cgcccggtcg cacaggtatc cacaagcgcg
     5521 gcccagccat cgatccgggt ctccttgggt cgaggagggc ccatgccggc cgcagtaagc
     5581 cgcaacacaa tgtggtcggc tgaagagtcg gacggccgcc cgacccggcc tctacaacca
     5641 gtcgttgcgg cggaacaacc agtacagccc taccgtgccg accacgatca gcagcacgct
     5701 ctgccacagt ccccacgccg acgagaaacc ccagtacggc aggttctgcc cgaaccagcc
     5761 ggtcaccgcc gtcggcaccg cgatgatcgc gccccatgag gcgagcttct tcatgatggt
     5821 gttcaaccgg gcgtcctgca ggctcaggtt cgtctcgaac accgaggcga ccatgtcgcg
     5881 caacgactcg gtccattccg ccgcccgcaa cacatggtcg tagaggtcgt tgaactcgcc
     5941 gtccaactcc ggcgatcgtt gccattcgcc cgacagccgg tgccgcatca cccccgcgac
     6001 cacctcgcgc ataggcagca ccacgcgccg cagccgcacc agcgccttgc gcagcgcgaa
     6061 ggtgcgctgc tgaacctcgc gggtgcgcac ggcgtcctcg aacagcaggt cctccaggtc
     6121 ttcgagcttg tcgtccatca cctgcacggc ctcgaaatga ccatcgacca gccagtcgag
     6181 cagtccgtgc acga
//
LOCUS       DAOSUG010000129         6156 bp    DNA     linear   ENV 10-JUN-2024
DEFINITION  MAG TPA_asm: Micropruina sp. isolate AMR_MDS_2195
            NODE_5961_length_6156_cov_3.742337, whole genome shotgun sequence.
ACCESSION   DAOSUG010000129 DAOSUG010000000
VERSION     DAOSUG010000129.1
DBLINK      BioProject: PRJNA925477
            BioSample: SAMN32864262
            Sequence Read Archive: SRR4236673
KEYWORDS    WGS; ENV; Metagenome Assembled Genome; MAG; Third Party Data; TPA;
            TPA:assembly.
SOURCE      Micropruina sp. (wastewater metagenome)
  ORGANISM  Micropruina sp.
            Bacteria; Bacillati; Actinomycetota; Actinomycetes;
            Propionibacteriales; Nocardioidaceae; Micropruina.
REFERENCE   1  (bases 1 to 6156)
  AUTHORS   Abdulkadir,N., Saraiva,J.P., Zhang,J., Stolte,S., Gillor,O.,
            Harms,H. and Rocha,U.
  TITLE     Genome-centric analyses of 165 metagenomes show that mobile genetic
            elements are crucial for the transmission of antimicrobial
            resistance genes to pathogens in activated sludge and wastewater
  JOURNAL   Microbiol Spectr 12 (3), e0291823 (2024)
   PUBMED   38289113
REFERENCE   2  (bases 1 to 6156)
  AUTHORS   Abdulkadir,N.
  TITLE     Direct Submission
  JOURNAL   Submitted (24-MAR-2023) Environmental Microbiology, Helmholtz
            Center for Environmental Research -UFZ, Permoserstr 15, Leipzig
            04318, Germany
COMMENT     The annotation was added by the NCBI Prokaryotic Genome Annotation
            Pipeline (PGAP). Information about PGAP can be found here:
            https://www.ncbi.nlm.nih.gov/genome/annotation_prok/
            
            ##Genome-Assembly-Data-START##
            Assembly Method        :: SPAdes v. 3.11.1
            Genome Representation  :: Full
            Expected Final Version :: Yes
            Genome Coverage        :: Not Applicable
            Sequencing Technology  :: Illumina MiSeq
            ##Genome-Assembly-Data-END##
            
            ##Genome-Annotation-Data-START##
            Annotation Provider               :: NCBI
            Annotation Date                   :: 05/04/2023 04:57:10
            Annotation Pipeline               :: NCBI Prokaryotic Genome
                                                 Annotation Pipeline (PGAP)
            Annotation Method                 :: Best-placed reference protein
                                                 set; GeneMarkS-2+
            Annotation Software revision      :: 6.5
            Features Annotated                :: Gene; CDS; rRNA; tRNA; ncRNA
            Genes (total)                     :: 2,776
            CDSs (total)                      :: 2,750
            Genes (coding)                    :: 2,714
            CDSs (with protein)               :: 2,714
            Genes (RNA)                       :: 26
            tRNAs                             :: 24
            ncRNAs                            :: 2
            Pseudo Genes (total)              :: 36
            CDSs (without protein)            :: 36
            Pseudo Genes (ambiguous residues) :: 0 of 36
            Pseudo Genes (frameshifted)       :: 2 of 36
            Pseudo Genes (incomplete)         :: 32 of 36
            Pseudo Genes (internal stop)      :: 2 of 36
            ##Genome-Annotation-Data-END##
FEATURES             Location/Qualifiers
     source          1..6156
                     /organism="Micropruina sp."
                     /mol_type="genomic DNA"
                     /submitter_seqid="NODE_5961_length_6156_cov_3.742337"
                     /isolate="AMR_MDS_2195"
                     /isolation_source="Activated sludge of municipal
                     wastewater treatment plants"
                     /db_xref="taxon:2737536"
                     /environmental_sample
                     /geo_loc_name="Canada"
                     /lat_lon="49.25 N 123.1 W"
                     /collection_date="2015-06-16"
                     /metagenome_source="wastewater metagenome"
                     /note="metagenomic"
     gene            complement(373..1170)
                     /locus_tag="PLF56_05660"
     CDS             complement(373..1170)
                     /locus_tag="PLF56_05660"
                     /inference="COORDINATES: protein motif:HMM:NF019633.2"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_function: GO:0071949 - FAD binding [Evidence IEA];
                     GO_process: GO:0055072 - iron ion homeostasis [Evidence
                     IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="siderophore-interacting protein"
                     /protein_id="HOQ53083.1"
                     /translation="MAMYPTLMPKQPRMFTASVLRTERRSPTFQRVTIASDDLAGFEW
                     RGLDHWFRLFFPLRPDGPLLLPEVKGRQWWTSYLRIPAAERPHCSNYTVAGFRRGETI
                     AELDIDVVLHWHDGGLSGVAAWAVNATADSPVGLLDQGVLFDPPADTDEYVLVADESG
                     LPALRGILRDLPSDAVGTAIIEVPDAGDVHEVAAPEGLQVRWVPRDSSDDEVGHRALQ
                     ELQTCPTRPTAYAFVVGESRLATRGRRTLHRAGLPKNRITFSGFWRA"
     gene            1208..2224
                     /locus_tag="PLF56_05665"
     CDS             1208..2224
                     /locus_tag="PLF56_05665"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_012922736.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_function: GO:0042626 - ATPase-coupled transmembrane
                     transporter activity [Evidence IEA];
                     GO_function: GO:0140359 - ABC-type transporter activity
                     [Evidence IEA];
                     GO_process: GO:0006826 - iron ion transport [Evidence
                     IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="iron ABC transporter permease"
                     /protein_id="HOQ53084.1"
                     /translation="MTPTALATARAVRRKATLGLGLALLAVLVASLALGSNPISPARV
                     VQALLDPGNDPDAVVWGSRIPRTVLGLVIGFCLGVAGVVMQGQTGNPLADPGLFGVSS
                     GASLAVVLGAFVLGHNSMTASLFLAMIGALVASVGVFTIATLSRDLANPVPLAIAGTA
                     VSALLNAVISFLVLTNQSSLEAYRVWVVGSLSGRGLDGLLPALPVAAAGLVLALVNAR
                     SLNSLTLGTELAQGLGETLWQARLVGLAAITLTTAAAVALAGPIGFIGLTAPHLARAL
                     VGGNHYRLLPASGLIGAITLLACDVVGRVIGGQAEVPVGVVLAVLGGVAFVAIVRRTK
                     LASL"
     gene            2221..3276
                     /locus_tag="PLF56_05670"
     CDS             2221..3276
                     /locus_tag="PLF56_05670"
                     /inference="COORDINATES: protein motif:HMM:NF013219.2"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_component: GO:0016020 - membrane [Evidence IEA];
                     GO_function: GO:0022857 - transmembrane transporter
                     activity [Evidence IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="iron chelate uptake ABC transporter family
                     permease subunit"
                     /protein_id="HOQ53085.1"
                     /translation="MSTRQGVDLGLIGLPWRPRAFVVTLACLALLVAAFTFGLVAGSS
                     RMGVGDVWATLLGSGTRSFEFIVFELRLPRVSAAIGVGLCLGLAGALTQSFARNPLAT
                     PDILGVTSGASAGAVVAIVLGGGGYSVGASLLGVGIPVVATIGGLIAAAVVYGLSWRQ
                     GVDSFRLILIGIGVTAILGGLVNYLLVRAQITQATAATQWLVGSLSGVSWASVWPMVV
                     VLAVVVPFALSQSAPLTINQLGDEFSTGLGVAVQRHRLLVIVAAVVLASAAVSAAGPV
                     EFVAFVAPQVARRLARAPRPPLIASALVGAALVLIADVGARVAFGTEIPVGIVTALIG
                     APYLIWLITRPQLKERA"
     gene            3273..4133
                     /locus_tag="PLF56_05675"
     CDS             3273..4133
                     /locus_tag="PLF56_05675"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_014847057.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_function: GO:0005524 - ATP binding [Evidence IEA];
                     GO_function: GO:0016887 - ATP hydrolysis activity
                     [Evidence IEA];
                     GO_function: GO:0042626 - ATPase-coupled transmembrane
                     transporter activity [Evidence IEA];
                     GO_function: GO:0140359 - ABC-type transporter activity
                     [Evidence IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="ABC transporter ATP-binding protein"
                     /protein_id="HOQ53086.1"
                     /translation="MTTTTQPALVAEAATLGYDDHVVFNGIDLQIEAGTVTTLIGANG
                     CGKSTLLKAFGRLLTPSAGSIRLEGTPLHRLSARAVARRLAILPQKPLTPAATTVRDL
                     VMRGRHPHQTLLRPWSPQDAAKVEAALEATGMSELADRDVGSLSGGQLQRAWIALVLA
                     QDAPTILLDEPTTFLDLSHQLEVLRLVRTINARHGSTIVMVLHDLSLAARYSDRLVVL
                     HQGRVLADGEPWQVITPEVLDAAFGLEALVIADPASGRPLVVPLDPSGAHPAERAPRV
                     QRVPGDAQVA"
     gene            4154..5164
                     /locus_tag="PLF56_05680"
     CDS             4154..5164
                     /locus_tag="PLF56_05680"
                     /inference="COORDINATES: protein motif:HMM:NF013648.2"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="ABC transporter substrate-binding protein"
                     /protein_id="HOQ53087.1"
                     /translation="MLPPKNMRTLVLALLSVLALAACSSPSAGTSATSAGSATASSWT
                     YTDDTGNTITLPAKPQRIVAFADQALSLMSYGVKPAGIFARVDVKTDPRFAGADLGDV
                     KILGTSYGEIDLEALAELAPDLIVTAVYPSDRQGTLDPKSPYYGMKDLEQQKKIEAIA
                     KVAAVKVGGEGRAVIESNAKLALALGADQAKVDSDKAAFDAAAGKLTEAAKATDIEVT
                     QMYADADGVYLVKPDDEPQTQLYKSLGVKLTELRPDGNYYWDIYSWENAGKAMSGDVI
                     MLSPEGFQVADLKKQPTFAKDPALVAGQVYPNPYTAMDYSAQAKAMDVLTGYLTTAKK
                     LG"
     gene            5438..>6156
                     /locus_tag="PLF56_05685"
     CDS             5438..>6156
                     /locus_tag="PLF56_05685"
                     /inference="COORDINATES: protein motif:HMM:NF014753.2"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="dihydroxyacetone kinase subunit DhaK"
                     /protein_id="HOQ53088.1"
                     /translation="MKRLLNDGYDAVEELLDGYVKAHGDVVKLADNERVVVRTTLSAE
                     PKVGIIFGGGSGHEPLFLGYVGEGVADAAVIGNVNTSPDPIACYDAVTAVDQGKGCLY
                     LYGNYQGDVLNFDMGAEMASDDGIRVQTVRSTGDVYSAKDKADRRGVAGDLIVLMAAC
                     AVANTGADLATVKAAAEKANDNTYSMGVALSSSTLPVTGKPIFDIGPDDMEVGMGIHG
                     EPGIERKKLEPADAVVDEILG"
BASE COUNT          899 a         2156 c         2118 g          983 t
ORIGIN      
        1 cgtcctctcg gcggagcctt ccgtcctctc ggcggagcct tccgttatcc cggcggagcc
       61 ttccgttatc ccggcggagc cttccgttat cccggcggag cgttccgttc tcccggcgga
      121 gccttccgtc ctcccggcgg cacccttccg tcgtcccggc ggagcgttcc gtcctcccgg
      181 cggagcgttc cgtcctccag gcggagcgtt ccgtcatccc ggcggagcgt tccgtcctcc
      241 cggcggagcg ttccgtcctc ccggcggagc gttccgtcct cccggcgaac gccgggatct
      301 caccacgggc ccaaccgcac ctgagatccc ggggcacgcc cgggatggcg tgggctacac
      361 cgggctaaac cgctacgccc gccagaaacc cgagaaggtg atccggttct tgggcagccc
      421 ggcccggtgc aacgtccgcc tgccccgggt cgccaggcgc gattcgccga ccacgaaggc
      481 gtacgccgtg ggccgggtcg ggcacgtctg caactcctgc agcgcgcggt gacccacctc
      541 gtcgtcgctg ctgtcgcgcg gcacccaccg cacctgcaag ccctcgggag cggccacctc
      601 atgcacatcg ccggcgtccg gcacctcgat gatcgcggtg ccgaccgcgt cgctcggcag
      661 gtcgcgcaga atgcctcgca atgccggcag gccgctctcg tccgccacca gaacgtactc
      721 gtcggtgtcg gccggcgggt cgaacagcac gccctggtcg agcagtccga ccgggctgtc
      781 cgccgtggcg ttcaccgccc aggccgcgac gcccgacagc ccgccgtcgt gccagtgcag
      841 caccacgtcg atgtccagtt cagcgatggt ctcgccacgc cggaaccccg cgacggtgta
      901 gttgctgcag tgtggacgct cggccgcggg aatccgcagg tacgaggtcc accactgacg
      961 acccttcacc tcgggcagca gcaggggacc gtccggtcgc agcggaaaga acaaccggaa
     1021 ccagtgatcc agcccccgcc actcgaaacc ggccaggtcg tccgaggcga tcgtgacgcg
     1081 ctgaaaggtc ggcgatcggc gctcggtgcg cagcacggac gcggtgaaca tgcgcggctg
     1141 cttcggcatc aacgtcgggt acatggccat gcagaagcct tccagaaggt taggttaggc
     1201 ttaccatatg acacccacgg cgctcgcgac cgctcgcgcc gtgcggcgga aggcaacgct
     1261 cggtctgggc ctggcgctgc tggcggtgct ggtggccagc ctcgcattgg gcagcaatcc
     1321 gatctcaccg gcacgggtgg tgcaggcact gctcgatccg ggcaacgacc ccgacgcggt
     1381 ggtctggggc tcacgcatcc ctcgcacggt gctgggcctg gtgatcggat tctgcctcgg
     1441 cgtcgccggg gtggtgatgc agggccagac cggtaacccg ttggccgacc cggggctgtt
     1501 cggggtctca tccggggcca gcctggccgt ggtgctgggc gccttcgtgc tgggtcacaa
     1561 ctcgatgacc gcgtcgctgt tcctggccat gatcggggcg ctggtggcca gcgtcggggt
     1621 attcaccatc gccacgctca gccgcgacct ggccaacccc gtgccactgg ccatcgccgg
     1681 caccgccgtc agcgccctgc tcaacgcggt catctcgttt ctggtgctga ccaatcagag
     1741 ttcgctcgag gcctatcgcg tgtgggtggt cggttcgctg tcgggacggg gcctggacgg
     1801 gctgttgccc gcactgccgg tggccgcggc agggctggtg ctggctttgg tcaacgcacg
     1861 ctcgctgaac agcctgaccc tgggcaccga gttggcgcag ggactgggcg agaccctgtg
     1921 gcaggcccgg ctggtgggcc ttgccgccat cacgctgacc acggccgcgg ccgtcgcgct
     1981 ggccggcccg atcgggttca tcgggctgac cgccccgcac ctcgcgcgtg ccctggtcgg
     2041 cggcaaccac taccggctgc tgcccgcctc ggggctgatc ggggcgatca cactgttggc
     2101 ctgcgacgtg gtcggccggg tgatcggcgg gcaggccgag gtgccggtcg gtgtcgtcct
     2161 ggccgtgctg ggcggggtgg cgttcgtcgc gatcgtccgg cgcacgaagt tggcgtcgct
     2221 gtgagcacgc gccagggcgt cgacctgggg ctgatcggcc tgccctggcg gccgcgggca
     2281 ttcgtcgtga cgctggcctg cctcgcgctc ctcgtggcag cgttcacctt cggactggtc
     2341 gccggctcgt cccggatggg ggtcggcgac gtgtgggcca cactgctcgg ctccggcacc
     2401 cgctccttcg agttcatcgt gttcgaactg cggctgccgc gggtctcggc ggcgatcggg
     2461 gtgggactgt gcctggggct ggccggcgcg ctgacccagt cgttcgcccg caatccgctg
     2521 gccacgcccg acattctggg ggtcacctcg ggcgcgtccg ccggtgcggt ggtggcgatc
     2581 gtgctcggcg gcgggggcta ctcggtcggc gcctcactgc tcggcgtggg catacccgtg
     2641 gtggcaacga tcggcgggtt gatcgcggcc gccgtcgtct acgggctgtc gtggcgccag
     2701 ggggtggaca gctttcggct gatcctgatc ggcatcggcg tcaccgcgat tctgggcggg
     2761 ctggtcaact atctgctggt gcgggcccag atcacgcagg ccaccgccgc cacccaatgg
     2821 ctggtcggca gcctgtcggg ggtctcgtgg gccagcgtgt ggccgatggt ggtggtcttg
     2881 gccgtcgtgg tgccgttcgc gctcagccag tcggcgccgc tgaccatcaa ccagttgggc
     2941 gacgagttct cgaccggact gggcgtggcg gtgcagcggc accggttgct ggtcatcgtg
     3001 gccgccgtgg tgctggcgag cgcggcggtc tcggcggccg ggccggtcga gttcgtggcc
     3061 ttcgtcgccc cgcaggtggc acggcggctg gcccgcgcgc cccgtccgcc gttgatcgcc
     3121 tcagcgctgg tgggcgccgc gctggtattg atcgcggacg tcggcgcccg ggtggcgttc
     3181 ggcaccgaga tcccggtcgg catcgtcacg gccctgatcg gtgcccccta cctgatctgg
     3241 ctgatcaccc gtccccaact caaggagcgt gcatgaccac caccacccag ccggcgctcg
     3301 tcgccgaggc cgccacgctg ggctacgacg atcatgtggt gttcaatggg atcgacctgc
     3361 agatcgaagc cggcacggtg accacgctga tcggggccaa cggttgcggc aagtcgaccc
     3421 tgctcaaggc cttcggacga ctattgaccc ccagcgccgg gtcgatccgg ctggagggca
     3481 cgccgctgca ccgtctgtcg gcccgcgccg tggcccggcg gctggccatc ctgccgcaga
     3541 agccgctcac cccggccgcc accacggtgc gtgatctggt gatgcgtgga cgacaccccc
     3601 accagacctt gctgcggccc tggtcgcccc aggacgcggc caaggttgag gccgcgctgg
     3661 aggccaccgg catgagcgag ctggccgatc gcgacgtcgg ctcgctgtcg gggggccagc
     3721 tgcaacgcgc ttggatcgcg ctggtgctgg cccaggacgc tccgacgatc ctgctcgacg
     3781 agccgaccac ctttctcgac ctgagccacc aactcgaggt gctgcggctg gtgcgcacga
     3841 tcaacgcccg acacggcagc acgatcgtga tggtgctgca cgacctgagc ctggccgcgc
     3901 gctattccga tcggctggtg gtgctgcacc agggacgggt gctggctgat ggcgaaccgt
     3961 ggcaggtgat cacgcccgag gtgctggacg ccgcgttcgg cctcgaggcc ctggtgatcg
     4021 ccgacccggc ctcaggccgt ccgctggtgg tgccgctcga cccgtccggg gcgcacccgg
     4081 ccgagcgcgc gccgcgcgtc cagcgggtgc cgggtgatgc tcaggtggct taggacaggc
     4141 tagccttagt cgcatgcttc cacccaagaa catgcgcacc ctggtgctcg cactgctgtc
     4201 cgtgctggcc ctcgcagcct gcagcagccc gagcgccggc acgtccgcca cgtccgctgg
     4261 ctcggcaacg gccagcagct ggacctacac cgacgacacc ggcaatacga tcacgctgcc
     4321 cgccaagccg cagcgcatcg tcgccttcgc cgatcaggcc ctgtcgctga tgagctacgg
     4381 ggtgaagccg gccggtatct tcgcccgggt cgacgtcaag accgatcccc gcttcgccgg
     4441 ggccgacctc ggcgacgtga agatcctggg caccagctac ggcgagatcg acctggaggc
     4501 cctggctgaa ctggcccccg acctgatcgt caccgccgtg tacccgagcg accgccaagg
     4561 cacgctcgac ccgaaatcgc cgtactacgg catgaaggac ctcgagcagc agaagaagat
     4621 cgaggccatc gcgaaggtgg ccgcggtcaa ggtgggcggc gagggtcggg ccgtcatcga
     4681 gagcaacgcc aagctggcgc tcgcactcgg cgccgatcag gccaaggtcg actccgacaa
     4741 ggccgccttc gacgccgcgg cgggcaagct gaccgaggcg gcgaaggcca ccgacatcga
     4801 ggtcacccag atgtacgccg acgccgacgg tgtgtacctg gtcaagcccg acgacgagcc
     4861 ccagacgcag ctgtacaaga gcctcggcgt caaactcacc gaactgcggc ccgacggcaa
     4921 ctactactgg gacatctaca gctgggagaa cgcaggcaag gcgatgtccg gtgatgtgat
     4981 catgctgtcg cccgaaggtt ttcaggtcgc cgacctgaag aagcagccca ccttcgccaa
     5041 ggaccccgcc ctggtcgccg gccaggtgta ccccaatccg tacacggcca tggactactc
     5101 cgcgcaggcc aaggccatgg acgtgctcac cggctacctg accacggcca agaagctggg
     5161 ctgagacgct gcagccagcg catggtggcc ggcgtcaggt cgtgcacccg tcacagccac
     5221 gctgtggatt tgtgacttgt gctgtgaaaa tctcggtcga tccctcgtgc tccgtgggtc
     5281 cgcctgcgat aatcgaagcg cacgatcctg attgtggcac cgtcgccatc aggtgactgc
     5341 gcaccaggct caggggcccg acgacgcgac ccgcaactgc gtggcaacgt cgcccggcac
     5401 aaggcgcacg cgcgcaccac gacgaaagga ccaacccatg aagcgactgc tcaacgacgg
     5461 ctacgacgcg gttgaagaac tactggacgg ttacgtcaag gcccacggcg acgtggtgaa
     5521 gttggccgac aacgagcggg tcgtggtgcg cacgacgctc agcgccgagc ccaaggtggg
     5581 catcatcttc ggcggcggca gtggtcacga accgctgttc ctcggctacg tcggcgaagg
     5641 cgtggccgat gccgccgtga tcggcaacgt gaacacctca cccgacccga tcgcctgcta
     5701 cgacgcggtc accgcggtcg atcagggcaa gggttgcctc tatctgtacg gcaactacca
     5761 gggcgacgtg ctgaacttcg acatgggtgc cgagatggca tcggacgacg gcatccgggt
     5821 ccagaccgtg cggagcacag gagacgtcta ctccgccaag gacaaggccg atcgccgcgg
     5881 ggtggcgggt gacctgatcg tgctgatggc cgcgtgtgcc gtggccaaca ccggcgccga
     5941 cctcgccacc gttaaggcgg ccgccgagaa ggccaacgac aacacctact cgatgggcgt
     6001 cgccctgtcg tcgtccaccc ttcctgtcac cggcaagccg atcttcgaca tcggccccga
     6061 cgacatggag gtcggcatgg gcattcacgg cgagcccggc atcgagcgca agaagctcga
     6121 gccggccgac gctgtcgtgg acgagattct cggcat
//
LOCUS       DAOSUG010000130         6155 bp    DNA     linear   ENV 10-JUN-2024
DEFINITION  MAG TPA_asm: Micropruina sp. isolate AMR_MDS_2195
            NODE_5963_length_6155_cov_4.054590, whole genome shotgun sequence.
ACCESSION   DAOSUG010000130 DAOSUG010000000
VERSION     DAOSUG010000130.1
DBLINK      BioProject: PRJNA925477
            BioSample: SAMN32864262
            Sequence Read Archive: SRR4236673
KEYWORDS    WGS; ENV; Metagenome Assembled Genome; MAG; Third Party Data; TPA;
            TPA:assembly.
SOURCE      Micropruina sp. (wastewater metagenome)
  ORGANISM  Micropruina sp.
            Bacteria; Bacillati; Actinomycetota; Actinomycetes;
            Propionibacteriales; Nocardioidaceae; Micropruina.
REFERENCE   1  (bases 1 to 6155)
  AUTHORS   Abdulkadir,N., Saraiva,J.P., Zhang,J., Stolte,S., Gillor,O.,
            Harms,H. and Rocha,U.
  TITLE     Genome-centric analyses of 165 metagenomes show that mobile genetic
            elements are crucial for the transmission of antimicrobial
            resistance genes to pathogens in activated sludge and wastewater
  JOURNAL   Microbiol Spectr 12 (3), e0291823 (2024)
   PUBMED   38289113
REFERENCE   2  (bases 1 to 6155)
  AUTHORS   Abdulkadir,N.
  TITLE     Direct Submission
  JOURNAL   Submitted (24-MAR-2023) Environmental Microbiology, Helmholtz
            Center for Environmental Research -UFZ, Permoserstr 15, Leipzig
            04318, Germany
COMMENT     The annotation was added by the NCBI Prokaryotic Genome Annotation
            Pipeline (PGAP). Information about PGAP can be found here:
            https://www.ncbi.nlm.nih.gov/genome/annotation_prok/
            
            ##Genome-Assembly-Data-START##
            Assembly Method        :: SPAdes v. 3.11.1
            Genome Representation  :: Full
            Expected Final Version :: Yes
            Genome Coverage        :: Not Applicable
            Sequencing Technology  :: Illumina MiSeq
            ##Genome-Assembly-Data-END##
            
            ##Genome-Annotation-Data-START##
            Annotation Provider               :: NCBI
            Annotation Date                   :: 05/04/2023 04:57:10
            Annotation Pipeline               :: NCBI Prokaryotic Genome
                                                 Annotation Pipeline (PGAP)
            Annotation Method                 :: Best-placed reference protein
                                                 set; GeneMarkS-2+
            Annotation Software revision      :: 6.5
            Features Annotated                :: Gene; CDS; rRNA; tRNA; ncRNA
            Genes (total)                     :: 2,776
            CDSs (total)                      :: 2,750
            Genes (coding)                    :: 2,714
            CDSs (with protein)               :: 2,714
            Genes (RNA)                       :: 26
            tRNAs                             :: 24
            ncRNAs                            :: 2
            Pseudo Genes (total)              :: 36
            CDSs (without protein)            :: 36
            Pseudo Genes (ambiguous residues) :: 0 of 36
            Pseudo Genes (frameshifted)       :: 2 of 36
            Pseudo Genes (incomplete)         :: 32 of 36
            Pseudo Genes (internal stop)      :: 2 of 36
            ##Genome-Annotation-Data-END##
FEATURES             Location/Qualifiers
     source          1..6155
                     /organism="Micropruina sp."
                     /mol_type="genomic DNA"
                     /submitter_seqid="NODE_5963_length_6155_cov_4.054590"
                     /isolate="AMR_MDS_2195"
                     /isolation_source="Activated sludge of municipal
                     wastewater treatment plants"
                     /db_xref="taxon:2737536"
                     /environmental_sample
                     /geo_loc_name="Canada"
                     /lat_lon="49.25 N 123.1 W"
                     /collection_date="2015-06-16"
                     /metagenome_source="wastewater metagenome"
                     /note="metagenomic"
     gene            <1..2312
                     /locus_tag="PLF56_05690"
     CDS             <1..2312
                     /locus_tag="PLF56_05690"
                     /EC_number="4.1.1.71"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_002520558.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_function: GO:0004591 - oxoglutarate dehydrogenase
                     (succinyl-transferring) activity [Evidence IEA];
                     GO_function: GO:0016746 - acyltransferase activity
                     [Evidence IEA];
                     GO_function: GO:0030976 - thiamine pyrophosphate binding
                     [Evidence IEA];
                     GO_process: GO:0006099 - tricarboxylic acid cycle
                     [Evidence IEA]"
                     /codon_start=3
                     /transl_table=11
                     /product="multifunctional oxoglutarate
                     decarboxylase/oxoglutarate dehydrogenase thiamine
                     pyrophosphate-binding subunit/dihydrolipoyllysine-residue
                     succinyltransferase subunit"
                     /protein_id="HOQ53089.1"
                     /translation="IEYMHIQDNEQRAWIQKRVEVPHAPWPREEHLRILDRLNEAEIF
                     ETFLQTKFVGQKRFSLEGGESTIVLLDELCDAAANAELDEVVIGMPHRGRLNVLANIV
                     GKSYGQIFKEFEGSVDPRHAQGTGDVKYHLGAEGQFTALSGKTIKTSVAANPSHLEAV
                     DPVVEGIARAKQDQLTGAAGYPVLPILLHGDASFAGQGVVYETLQMSQLRPYKTGGTI
                     HVVVNNQVGFTTSPYDSRTSVYCTDVAKAIQSPIFHINGDDPEAVARIAQLAFEYRQR
                     FNKDVVLDVVCYRRRGHNEGDDPSFTQPRMYDLIEVKRSVRKLYTEALIGRGDISTED
                     AEAVLTRFRDGLESVFKKARDEAAEDDSYRKVPYYPPKLGKTQGTAISAETMAKVARA
                     HVTFPDGFTVHPKVLPQLQRRADGIANGPIDWATGELLAFGSLLLEGRPVRLVGQDTR
                     RGTFVQRFAAVVDRDTAEGYVPLNHLSDNQGTFHVYDSLLSEYASMGFEYGYSVAAPD
                     ALVCWEAQFGDFANGAQTIADEFIASGNAKWTQKSGVVLLLPHGYEGQGPDHSSMRIE
                     RWLQLCHEGALAVCQPSSPASYFHLLRTHAYVNWHRPVVIATPKSMLRNKMAVSQPRD
                     FTHGRWEPAQGDPTISDPASVSRILICSGKLRWDLVTARHEAGLDDRVAILSLERLYP
                     LPAKELAAQLAQYPKDADIRYVQDEPENQGAWWFVQQHLPEAVAAFLPGYRLVMRPIS
                     RPAASAPSVGSLKVHRAQQEDLLERAFAD"
     gene            2425..3573
                     /gene="galK"
                     /locus_tag="PLF56_05695"
     CDS             2425..3573
                     /gene="galK"
                     /locus_tag="PLF56_05695"
                     /EC_number="2.7.1.6"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_013863962.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_function: GO:0004335 - galactokinase activity [Evidence
                     IEA];
                     GO_process: GO:0006012 - galactose metabolic process
                     [Evidence IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="galactokinase"
                     /protein_id="HOQ53090.1"
                     /translation="MSLDLAVHEAFVTLTGRDPDSIWRAPGRINLIGEHTDYNDGFVL
                     PFALPQAAYLAAARRDDEVIRITSVDLGEQFEATLGELAPNQDGWHSYLAGVFWVLRE
                     AGYPIGGADLALASDVPIGAGLSSSAAIECVLVEALNDLYDLGIAPMDRAKLARRTEN
                     AYVGAPTGLMDQAASTLCTQGHALFLDCRSLEVQQVPLDLAAHGQAILVIDTLTRHSH
                     ADGEYATRRADCEQASALLGVPALRDVPIEGLDERLATLPDERLVRRARHVVTENQRV
                     LDACAALRADDLARLGELMTASHASMRDDYEITEASVDLAVEVALAAGAAGARMTGGG
                     FGGCVLVLAGQSRLDAIGAAVTAAFVESGRAAPATFVARPAAGAGRLT"
     gene            complement(3488..3925)
                     /locus_tag="PLF56_05700"
     CDS             complement(3488..3925)
                     /locus_tag="PLF56_05700"
                     /inference="COORDINATES: ab initio
                     prediction:GeneMarkS-2+"
                     /note="Derived by automated computational analysis using
                     gene prediction method: GeneMarkS-2+."
                     /codon_start=1
                     /transl_table=11
                     /product="hypothetical protein"
                     /protein_id="HOQ53091.1"
                     /translation="MRTTRPPGLIVAVTAAAMLGVVFVVFAVISAAGGHGAFSLQIGF
                     LLACYGALLIASAVGVWQQRMWARGPLVAFALMAGFGFGEYLLEQPWLWLLVALCLAA
                     VVGAVLPRTTRMLQQVRSAGQPPQPDGRRTWRERLGRTRRTPQ"
     gene            4195..4446
                     /locus_tag="PLF56_05705"
     CDS             4195..4446
                     /locus_tag="PLF56_05705"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_002526714.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_function: GO:0003677 - DNA binding [Evidence IEA];
                     GO_function: GO:0046872 - metal ion binding [Evidence
                     IEA];
                     GO_function: GO:0051536 - iron-sulfur cluster binding
                     [Evidence IEA];
                     GO_function: GO:0051539 - 4 iron, 4 sulfur cluster binding
                     [Evidence IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="WhiB family transcriptional regulator"
                     /protein_id="HOQ53092.1"
                     /translation="MDWRHRAACLTEDPELFFPVGNTGPALMQIQEAKKVCKRCEVRE
                     ACLQWALDAGQDHGVWGGLSEDERRALKRRAARSRVRAS"
     gene            complement(4472..5929)
                     /locus_tag="PLF56_05710"
     CDS             complement(4472..5929)
                     /locus_tag="PLF56_05710"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_002521538.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="sensor histidine kinase"
                     /protein_id="HOQ53093.1"
                     /translation="MLTMAEVIDARTSLDHRDVAWLEHLVAEWHLLADLAFSDLILWV
                     PDVDDNVFWACAQVRPTTGPTALEDDVVGERIHYDDEHQVTEAYMSHEIVLTSGNQLS
                     AGIPVDVHAIPVVRQDRVIGVVEAHTNRMGVRAPGALEDAYLEAAEVLSGMVHRCEYP
                     TGMDKSVPWVSPRVGDGMIRVGSDGIVSYASPNAVSAYRRLGLTADLEGENLAEVTMA
                     LLRNRAPVEMSLPNVLRGHGQQEVEVETAEVQLRMRIIRLNDDVGAVGQLVLCRDTTE
                     LRSRERQLVTKDATIREIHHRVKNNLQTVAALLRLQARRISLPEAKGALSDAMKRVGA
                     IAVVHEILSQAFDTSVKFDDVADRLLRMVVDVAATGDPVQLRREGTFGYVPADVATSL
                     SLVLTELCQNAIEHGLGEHRGVVWVKPVQADGRLVVEVSNEGEPLPPDFELGATNSLG
                     LSIVTTLVDDLGGEFTLTSDESGPGTVARVSIPLG"
BASE COUNT         1015 a         2118 c         2091 g          931 t
ORIGIN      
        1 gcatcgagta catgcacatt caggacaacg agcagcgggc ctggatccag aagcgcgtcg
       61 aggtgccgca cgccccctgg ccgcgcgagg agcacctacg cattctcgac cggctcaacg
      121 aggccgagat cttcgagacg ttcctgcaga ccaagttcgt cggccagaaa cgcttcagcc
      181 tcgagggcgg tgagtccacc atcgtgctgc tggacgagct gtgtgacgcg gccgcgaacg
      241 ccgagctgga cgaggtcgtc atcggcatgc cgcaccgggg ccggctcaac gtgctggcca
      301 acatcgtcgg caagagctac ggccagatct tcaaggagtt cgagggcagc gtcgacccgc
      361 ggcacgcgca gggcaccggc gacgtcaagt accacctggg tgccgagggc cagttcaccg
      421 cgctgtcggg caagacgatc aagacctcgg tggccgccaa ccccagccat ctcgaggcgg
      481 tcgatccggt ggtcgagggc atcgcccggg ccaagcagga ccagctcacc ggggccgcgg
      541 ggtatccggt gctgccgatc ctcctgcacg gcgacgcgtc gttcgcgggg cagggagtgg
      601 tgtacgagac gctgcagatg agccagctgc gtccctacaa gaccggcggc accatccacg
      661 tggtggtcaa caaccaggtc gggttcacca cctcgcccta cgactcgcgc acctcggtgt
      721 actgcaccga cgtggccaag gccattcagt cgccgatctt ccacatcaac ggcgacgacc
      781 ccgaagccgt ggcccggatc gcgcaactcg ccttcgagta ccggcagcgg ttcaacaagg
      841 acgtcgtgct cgacgtggtc tgctaccgcc gccgcggtca caacgagggc gacgacccga
      901 gcttcaccca gccgcgcatg tacgacctga tcgaggtcaa gcgcagcgtc cgcaagctgt
      961 acaccgaggc cctgatcggc cgcggcgaca tcagcaccga agacgccgag gccgtgttga
     1021 ccaggttccg cgacggcctc gagagcgtgt tcaagaaggc ccgcgacgag gccgccgaag
     1081 acgactcgta ccgcaaggtg ccctactacc ccccgaagct gggcaagacc cagggcaccg
     1141 cgatctcggc cgaaacgatg gccaaggtgg cccgcgccca cgtcacgttc ccggacggct
     1201 tcacggtgca ccccaaggtg ctgccgcagc tgcagcgccg cgccgacggc atcgccaacg
     1261 gaccgatcga ctgggccacc ggcgaactgc tcgctttcgg ttcgctgctg ctggagggcc
     1321 gtccggtgcg cctggtcggc caggacaccc gacggggcac tttcgtgcag cggttcgccg
     1381 cggtggtcga ccgtgacacc gccgaggggt acgtgccgct caatcacctg tccgacaacc
     1441 agggcacgtt ccatgtgtac gactccctgc tcagcgagta cgcctcgatg ggcttcgagt
     1501 acggctattc ggtggcggcc ccggacgcgc tggtgtgctg ggaggcccag ttcggtgact
     1561 tcgccaacgg cgcacagacc atcgccgatg agttcatcgc ctcgggcaac gccaaatgga
     1621 cccagaagtc gggcgtcgta ctgctgctgc cgcacgggta tgaggggcag gggcccgacc
     1681 actcgtccat gcgtatcgag cgctggctgc agctgtgtca cgagggcgcg ttggcggttt
     1741 gccagccgtc ctccccggcg agctactttc acctgctgcg cacccacgcc tacgtcaact
     1801 ggcaccgtcc ggtggtgatc gcgacgccca agtcgatgct gcgcaacaag atggcggtct
     1861 cgcagccgcg cgacttcacc catgggcgct gggagcccgc gcagggcgac cccacgatca
     1921 gcgatccggc ctcggtgtcg cgcatcctga tctgctcggg caagctgcgc tgggatctgg
     1981 tcaccgcccg gcacgaagcc ggtctggacg atcgggtggc gatcctgtcc ctggagcggc
     2041 tctacccgct gccggccaag gagctggccg cccagctggc gcaatacccc aaggacgccg
     2101 acattcgcta cgtgcaggac gagcccgaga accagggtgc ctggtggttc gtccagcagc
     2161 acctgcccga ggcggtcgca gcgttcctgc ccggctaccg gctggtgatg cggccgatca
     2221 gccgcccggc ggcctctgcc ccgtccgtcg gctcgctgaa ggtgcacagg gcccaacagg
     2281 aggatctgtt ggagcgcgcg ttcgccgact gacggctgca ccggccgaca tcggcggcac
     2341 cgtgcacggc gccgggggct gcggcgctac gctacgctcg acgaggtcgt gagcggcctc
     2401 cgaagcgatc aaggagatgt gcggatgagc ttggatctgg ctgtgcacga ggcgtttgtc
     2461 acgctgaccg gacgtgaccc ggacagcatc tggcgggcac ccggccggat caacctgatc
     2521 ggtgagcaca ccgactacaa cgacggtttc gtgctgccgt tcgcgctgcc gcaggccgcc
     2581 tacctggccg ccgcgcgccg cgacgacgag gtgatccgga tcacctcggt cgacctcggt
     2641 gaacagttcg aggccaccct gggcgagctg gcgccgaacc aggacggctg gcacagctac
     2701 ctcgccggtg tgttctgggt gctgcgtgag gccggttacc cgatcggtgg ggccgatctg
     2761 gcactggcct cggacgtgcc gatcggagcc gggttgtcat cgtccgccgc catcgagtgc
     2821 gtgctcgtcg aggccctgaa cgacctgtac gacctgggta tcgcgccgat ggatcgggcc
     2881 aagctggctc ggcgcaccga gaacgcctac gtgggtgccc cgaccggcct gatggaccag
     2941 gccgcctcca cgctgtgcac ccagggccac gccctgttcc tggactgccg ctcgctcgaa
     3001 gtgcagcagg tgccgctcga cctggcggcg cacgggcagg cgatcctggt gatcgacacc
     3061 ctgacccggc acagccatgc cgacggcgag tacgccaccc gccgggccga ttgcgaacag
     3121 gcgtcggcgt tgttgggcgt gcctgcgctg cgcgacgtgc cgatcgaagg gctggacgag
     3181 cggttggcca ccttgccgga cgagcggctg gtgcgccgcg cgcgccacgt ggtgaccgag
     3241 aatcagcggg tgctggatgc gtgcgcggcc ctgcgcgcgg acgacctggc ccggctgggc
     3301 gagctgatga ccgcctcaca cgcctcgatg cgggacgact acgagatcac cgaggcgtcg
     3361 gtggatctgg cggtcgaggt ggcgctcgca gcgggtgcgg ccggggcgcg gatgaccggc
     3421 ggaggcttcg gaggctgcgt gctggtgttg gccggccagt cgcggctgga cgcgatcggc
     3481 gccgccgtca ctgcggcgtt cgtcgagtcc ggccgagccg ctcccgccac gttcgtcgcc
     3541 cgtccggctg cgggggctgg ccggctgacc tgacctgctg cagcatgcgg gtggtcctcg
     3601 gcagcaccgc accgacgacg gcggccaggc acagggccac cagcagccac agccagggct
     3661 gttcgagcag gtactccccg aacccgaagc cggccatcag cgcgaaggcg accaacgggc
     3721 cgcgcgccca catgcgctgc tgccacacgc ccaccgccga ggcgatcagc agcgcgccgt
     3781 agcaggccag cagaaagccg atctgcaggc tgaacgcgcc atgcccgccg gcggcgctga
     3841 tcaccgcgaa caccacgaag acgacgccga gcatcgccgc agcggtgacg gcgacgatga
     3901 ggcccggggg gcgagtggtg cgcacgtccc cacggtagcg ggacgggtcg ggacgacagg
     3961 ggttgggcac gcccgtgaac cctccaccga tgcgcgcacc ggtccacgag cggctcacaa
     4021 ccaggtacct cggacgggaa tatgtccaga gatccgttca tttactgaaa ggcaccttgt
     4081 acatagccac taggcttgcc aggctagtct cgtagattcg tgcgctgccc cagttggtgg
     4141 cgcgacgccc agagagggca catgactttt ccacaagaag gagttggaag ccgcatggat
     4201 tggcgccatc gcgctgcctg cctcaccgag gacccggagc tgttctttcc ggtcggcaac
     4261 accggacccg ccctgatgca gattcaggag gccaagaagg tgtgcaagcg ttgtgaggtt
     4321 cgtgaggcat gcctgcagtg ggccctcgac gcgggtcagg atcacggtgt ctggggtggc
     4381 ctgagcgaag acgagcgacg ggcactcaag cgtcgcgccg cccggtcgcg ggtgcgcgcc
     4441 agctagcgaa cgccctcggc cggcagacgg ttcaccccag cggaatcgag acccgggcca
     4501 ccgttcccgg ccccgattcg tccgaggtca gggtgaactc accgcccagg tcgtccacca
     4561 gcgtggtgac gatcgaaagg cccaggctgt tggtcgcgcc caactcgaag tcgggcggca
     4621 ggggttcgcc ctcgttgctg acctcgacca ccaggcgccc atcggcctgg acgggcttca
     4681 cccagaccac gccgcgatgc tcgcccaggc cgtgctcgat cgcgttctgg cacagctcgg
     4741 tgagcaccag cgacaagctg gtcgccacgt cggccggcac gtagccgaag gtgccctcgc
     4801 ggcgcagctg cactggatca cccgtggcgg cgacgtccac caccatgcgc agcaggcggt
     4861 cggccacatc gtcgaacttc accgaggtgt cgaaggcctg ggacaggatc tcgtgcacca
     4921 ccgcgatcgc gccgacccgt ttcatcgcgt cgctgagagc gcccttggcc tccggcagcg
     4981 agatgcgccg ggcctgcaac cgcagcagtg cggcgaccgt ctggaggttg ttctttaccc
     5041 ggtggtggat ctcgcggatc gttgcgtcct tggtgaccag ttggcgctcg cggctgcgca
     5101 gttcggtggt gtcgcggcac agcaccagtt ggcccaccgc gcccacatcg tcgttgagcc
     5161 tgatgatgcg catgcgcagt tgcacctcgg cggtctcgac ctcgacctct tgctggccgt
     5221 ggccgcgcag cacgttcggc agtgacatct cgaccggcgc acggttgcgc agcagtgcca
     5281 tcgtcacctc ggccaggttc tcgccctcga ggtcggcggt gaggcccagc cggcggtagg
     5341 cgctgaccgc gttggggctg gcgtagctga cgatgccgtc gctgcccacc ctgatcatgc
     5401 cgtcaccgac ccgcggcgac acccagggca ccgacttgtc catgccggtc gggtactcgc
     5461 aacggtgcac catgcccgac agcacctcgg ctgcttcgag ataggcgtct tcgagtgctc
     5521 cgggggcacg tacacccatg cggttggtgt gcgcctcgac caccccgatc acgcggtcct
     5581 ggcgcaccac cggaatggcg tgcacatcga ccggaatgcc cgcgctgagc tgattaccgc
     5641 tggtcagcac gatctcgtgc gacatgtagg cctcggtgac ctggtgctcg tcgtcgtagt
     5701 ggattctctc gccgacgacg tcgtcctcga gtgcggtggg gccggtggtc ggacgcacct
     5761 gtgcgcaggc ccagaagacg ttgtcgtcga cgtccggcac ccacaggatc aggtcggaga
     5821 aggccagatc ggccagcaga tgccactcgg cgaccagatg ctccagccac gcgacgtccc
     5881 gatggtcgag gctggtgcgc gcgtcgatca cctcggccat cgtcagcatg cccgccaccc
     5941 tatggcagaa tggacaactg cgccgacggc gccccacaga cacgatgaag gagaccgaca
     6001 tgggcaaggg tggacgcaag cgtcgttccc gcaagaagaa caaggcgaat cacggcaagc
     6061 gtcccaacgc ctgatcgcaa cgccgacagc ccccgcatcc tgtggtgcgg gggctgtggt
     6121 gtgctgccga aaccgcgaac gcccgcctaa gtatc
//
LOCUS       DAOSUG010000131         6152 bp    DNA     linear   ENV 10-JUN-2024
DEFINITION  MAG TPA_asm: Micropruina sp. isolate AMR_MDS_2195
            NODE_5966_length_6152_cov_3.625226, whole genome shotgun sequence.
ACCESSION   DAOSUG010000131 DAOSUG010000000
VERSION     DAOSUG010000131.1
DBLINK      BioProject: PRJNA925477
            BioSample: SAMN32864262
            Sequence Read Archive: SRR4236673
KEYWORDS    WGS; ENV; Metagenome Assembled Genome; MAG; Third Party Data; TPA;
            TPA:assembly.
SOURCE      Micropruina sp. (wastewater metagenome)
  ORGANISM  Micropruina sp.
            Bacteria; Bacillati; Actinomycetota; Actinomycetes;
            Propionibacteriales; Nocardioidaceae; Micropruina.
REFERENCE   1  (bases 1 to 6152)
  AUTHORS   Abdulkadir,N., Saraiva,J.P., Zhang,J., Stolte,S., Gillor,O.,
            Harms,H. and Rocha,U.
  TITLE     Genome-centric analyses of 165 metagenomes show that mobile genetic
            elements are crucial for the transmission of antimicrobial
            resistance genes to pathogens in activated sludge and wastewater
  JOURNAL   Microbiol Spectr 12 (3), e0291823 (2024)
   PUBMED   38289113
REFERENCE   2  (bases 1 to 6152)
  AUTHORS   Abdulkadir,N.
  TITLE     Direct Submission
  JOURNAL   Submitted (24-MAR-2023) Environmental Microbiology, Helmholtz
            Center for Environmental Research -UFZ, Permoserstr 15, Leipzig
            04318, Germany
COMMENT     The annotation was added by the NCBI Prokaryotic Genome Annotation
            Pipeline (PGAP). Information about PGAP can be found here:
            https://www.ncbi.nlm.nih.gov/genome/annotation_prok/
            
            ##Genome-Assembly-Data-START##
            Assembly Method        :: SPAdes v. 3.11.1
            Genome Representation  :: Full
            Expected Final Version :: Yes
            Genome Coverage        :: Not Applicable
            Sequencing Technology  :: Illumina MiSeq
            ##Genome-Assembly-Data-END##
            
            ##Genome-Annotation-Data-START##
            Annotation Provider               :: NCBI
            Annotation Date                   :: 05/04/2023 04:57:10
            Annotation Pipeline               :: NCBI Prokaryotic Genome
                                                 Annotation Pipeline (PGAP)
            Annotation Method                 :: Best-placed reference protein
                                                 set; GeneMarkS-2+
            Annotation Software revision      :: 6.5
            Features Annotated                :: Gene; CDS; rRNA; tRNA; ncRNA
            Genes (total)                     :: 2,776
            CDSs (total)                      :: 2,750
            Genes (coding)                    :: 2,714
            CDSs (with protein)               :: 2,714
            Genes (RNA)                       :: 26
            tRNAs                             :: 24
            ncRNAs                            :: 2
            Pseudo Genes (total)              :: 36
            CDSs (without protein)            :: 36
            Pseudo Genes (ambiguous residues) :: 0 of 36
            Pseudo Genes (frameshifted)       :: 2 of 36
            Pseudo Genes (incomplete)         :: 32 of 36
            Pseudo Genes (internal stop)      :: 2 of 36
            ##Genome-Annotation-Data-END##
FEATURES             Location/Qualifiers
     source          1..6152
                     /organism="Micropruina sp."
                     /mol_type="genomic DNA"
                     /submitter_seqid="NODE_5966_length_6152_cov_3.625226"
                     /isolate="AMR_MDS_2195"
                     /isolation_source="Activated sludge of municipal
                     wastewater treatment plants"
                     /db_xref="taxon:2737536"
                     /environmental_sample
                     /geo_loc_name="Canada"
                     /lat_lon="49.25 N 123.1 W"
                     /collection_date="2015-06-16"
                     /metagenome_source="wastewater metagenome"
                     /note="metagenomic"
     gene            complement(<1..1320)
                     /locus_tag="PLF56_05715"
     CDS             complement(<1..1320)
                     /locus_tag="PLF56_05715"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_013865210.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="DNA recombination protein RmuC"
                     /protein_id="HOQ53094.1"
                     /translation="MLRGKIKGMQTVWVLVAGVLGAAIGGVLVRLVLQARAGSVRSHD
                     EAAFAAARSEVSEARREAADARAEAADARADAAAARAELARNATDAERFRADVADARA
                     EAAQALSEAAEVSAQLAAAQAQRDAALARSVELSADREAMVAQFKVLSSETLERQGKT
                     AEANAEHRLRATELLMKPVRESLEAFNQRLGDVEKERVRMTTELAGQVTAVRDTGERL
                     RSETRALITALRKPHVRGNWGEQQLKRVVEVAGMVEHCDFLTQQTTSTSADQVIRPDL
                     KVLLDGGRFVWVDSKVPLSAFLDAYEADDDAVRDAGLQQFAKNVKTHIDQLSGKSYFK
                     SDYGTPEFVVLFMPSEALAAEALSQLPDLHEYAATRSIILATPTTLIALLRAVAYGWR
                     QAGLAESAAQVSELARELYDRLGTLGGHFDRIGRSLTASVRAYNEAVG"
     gene            1478..1687
                     /locus_tag="PLF56_05720"
     CDS             1478..1687
                     /locus_tag="PLF56_05720"
                     /inference="COORDINATES: ab initio
                     prediction:GeneMarkS-2+"
                     /note="Derived by automated computational analysis using
                     gene prediction method: GeneMarkS-2+."
                     /codon_start=1
                     /transl_table=11
                     /product="hypothetical protein"
                     /protein_id="HOQ53095.1"
                     /translation="MTESAESPHLKPNTYEMAPEDKGEKIGYDAADPKFSDEGYTAEG
                     VLRHRNEQAPWGRTAEGEKGDRPDQ"
     gene            1707..2957
                     /locus_tag="PLF56_05725"
     CDS             1707..2957
                     /locus_tag="PLF56_05725"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_015069785.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_function: GO:0004177 - aminopeptidase activity
                     [Evidence IEA];
                     GO_function: GO:0008270 - zinc ion binding [Evidence IEA];
                     GO_process: GO:0006508 - proteolysis [Evidence IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="M18 family aminopeptidase"
                     /protein_id="HOQ53096.1"
                     /translation="MSRIDGLAGFISASPSSFHAARSAAAALVEAGFTELAESDPVSG
                     EPGGYVMVRDGSVLAWRLPERLDQPCFHLVGAHTDSPGFVLKPRPTSHTLGWQQAGME
                     VYGGPLPNSWLDREFGLAGRLVTASGEQVLVATGPLLRVPQVAPHLDRSVHEKLLLDR
                     QHHLHPIFAVSRPDADILAVVAQTAGLRQEEVWGHDLCAFVTEPPARIGLDGDLFASA
                     RLDNLASVYAGVRALADATDLRRIAVLACFDHEEVGSASATGASGPLLATLLLRIGDA
                     LGWSVQTAAAARARSLLVSADAGHAVHPNYAQLHDPDHRPLLNDGPLIKFNANQRYAT
                     DAVGEAAWRRACAEAGIPTQDFVSNNAVPCGSTIGPLAATRLGIRTVDVGIPLLSMHS
                     ARELCGVRDPGWLAGGLHAFYCAS"
     gene            complement(3099..3479)
                     /locus_tag="PLF56_05730"
     CDS             complement(3099..3479)
                     /locus_tag="PLF56_05730"
                     /inference="COORDINATES: ab initio
                     prediction:GeneMarkS-2+"
                     /note="Derived by automated computational analysis using
                     gene prediction method: GeneMarkS-2+."
                     /codon_start=1
                     /transl_table=11
                     /product="hypothetical protein"
                     /protein_id="HOQ53097.1"
                     /translation="MHQTMGAPDQHHTTSHSLNEWYRGVHNGRFVVLMPADDIVELGL
                     VNGQPVDEFSEWPAEPDRVLRAYRVGPAPPSRAAPRCTFPRPTCWCRATRSIGSAHTS
                     TSKRVTIRVERGAMAVQGGRPVAV"
     gene            complement(3696..3878)
                     /locus_tag="PLF56_05735"
     CDS             complement(3696..3878)
                     /locus_tag="PLF56_05735"
                     /inference="COORDINATES: ab initio
                     prediction:GeneMarkS-2+"
                     /note="Derived by automated computational analysis using
                     gene prediction method: GeneMarkS-2+."
                     /codon_start=1
                     /transl_table=11
                     /product="hypothetical protein"
                     /protein_id="HOQ53098.1"
                     /translation="MTGPLDPLARVFDHPPKQDFTDTELEIKAPMDYAADVEAVEKAM
                     LPMDQHLGRRRRRRSR"
     gene            complement(3875..4738)
                     /gene="fdhD"
                     /locus_tag="PLF56_05740"
     CDS             complement(3875..4738)
                     /gene="fdhD"
                     /locus_tag="PLF56_05740"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_013862855.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_function: GO:0016783 - sulfurtransferase activity
                     [Evidence IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="formate dehydrogenase accessory
                     sulfurtransferase FdhD"
                     /protein_id="HOQ53099.1"
                     /translation="MGRITARRPVVSIALTSGATRRVDVLAVEEPLELRVGGRALAVT
                     MRTPGSDVELAAGFLVSEGVIARGGDFRSAIHCGGPGSGGEVNTYNVLDVTLAAEVAP
                     PEPNRNFYTTSSCGVCGKASIDAIRTVSCYDVANDATCVTAETLTRIPTLLRRHQAVF
                     DKTGGLHAAALFDAASGELLVLREDVGRHNAVDKVIGWAVLADRLPLSGTVLQVSGRA
                     SFELVQKAVMAGIPALAAISAPSSLAVELAESAGLTLVGFLRGESMNVYSRADRVVVM
                     APSELPEEAHA"
     gene            4923..>6152
                     /locus_tag="PLF56_05745"
     CDS             4923..>6152
                     /locus_tag="PLF56_05745"
                     /inference="COORDINATES: protein motif:HMM:NF013791.2"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_component: GO:0016020 - membrane [Evidence IEA];
                     GO_component: GO:0070069 - cytochrome complex [Evidence
                     IEA];
                     GO_function: GO:0009055 - electron transfer activity
                     [Evidence IEA];
                     GO_function: GO:0046872 - metal ion binding [Evidence
                     IEA];
                     GO_process: GO:0019646 - aerobic electron transport chain
                     [Evidence IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="cytochrome ubiquinol oxidase subunit I"
                     /protein_id="HOQ53100.1"
                     /translation="MDFLDPLLLARWQFGLTTLYHFIFVPLTLGMSLSVAIFQTAWVR
                     TGNYKWLSLTRFFGKIFLINFAMGVVTGIVQEFQFGMNWSAYSRFVGDVFGAPLAFEG
                     LMAFFFEATFIGLWIFGWDRLPKKLHLATLWIATFGAWASAYFILAANAFMQNPVGYV
                     MAQDGRRAEMADFGALLTNRVALAAFPHTLLASLMFAAGVIIAVAAWHLARRQHHETM
                     TTALKYGCWLMIGAFLGTALSGDQLSLQMVATQPMKMAAAEALFDTACGADASFSIFT
                     LGTPDGSGEVWSLRVPYLLALLSTHEFAGCVEGINNLNAQYAAQWPQLAAHVDGVFNP
                     IPWITYWSFRWMMGLGLMATAVAVVGLWVTRTKAAKPPAQWMWKVAVWSAPLALLAIL
                     VGWIFTEMGRQPWIVFSL"
BASE COUNT          929 a         2127 c         2074 g         1022 t
ORIGIN      
        1 gccgaccgcc tcgttgtagg cgcgcacgga cgcggtgagc gagcgtccga tgcggtcgaa
       61 atgaccgccc agcgtgccca gccggtcata tagttcgcgg gccaactcgg acacctgcgc
      121 cgccgactcg gccaggcccg cctggcgcca gccgtaggcg acggcgcgca acagggcgat
      181 cagtgtggtc ggcgtggcca aaatgatgct gcgggtggcc gcgtactcgt gcaggtcggg
      241 cagctggctg agagcctcgg cggccaacgc ctcactcggc atgaacagca cgacgaactc
      301 aggcgtgccg tagtcgctct tgaaatacga cttgccggac agctggtcga tgtgagtttt
      361 gacgttcttg gcgaactgct gcaacccggc gtcgcggacg gcgtcgtcgt cggcctcgta
      421 cgcgtcgaga aaggcgctca gcggcacctt ggagtcgacc cagacgaagc gaccaccgtc
      481 gagcaacacc ttcaggtctg gacgaatcac ctgatcggcg gacgtgctgg tggtctgctg
      541 cgtgaggaag tcgcagtgct cgaccatgcc ggccacctcg accacgcgct tgagctgctg
      601 ctcgccccag ttgccccgca cgtgcggctt gcgcagcgcg gtgatcagcg cccgggtctc
      661 ggaccgcagc cgctcgccgg tgtcgcgcac ggcggtgacc tggccggcca gctcggtggt
      721 catgcgcacc cgctctttct cgacgtcacc cagccgctga ttgaatgcct ccagcgactc
      781 gcggaccggc ttcatcagca actcagtggc gcgaagccga tgctcggcgt tcgcctcggc
      841 cgtcttgccc tgccgctcga gcgtttcgct cgacagcacc ttgaactggg cgaccatcgc
      901 ttcgcggtcg gcgctgagct cgactgagcg agccagtgcc gcgtcgcgct gtgcctgcgc
      961 ggccgccaac tgggccgaca cctcggcggc ttcgctcagg gcctgcgccg cttcggcccg
     1021 cgcgtcggcc acgtcggcgc gaaagcgttc agcgtcggtg gcgttgcggg ccagctcggc
     1081 gcgggcagcg gcggcgtcgg cccgggcgtc ggcggcctcg gcccgggcgt cggcagcttc
     1141 gcgcctggcc tcggacacct cggaacgtgc tgccgcgaac gccgcctcat cgtgcgatcg
     1201 gacgctgccc gccctcgcct gcagaaccag gcgcacgagc acaccgccga tcgcggcacc
     1261 cagcacgccc gccaccaaga cccacaccgt ctgcatgcct ttgatcttgc cgcgcagcac
     1321 cgacaaaacc cgtgaccgac tcgggcgtgg gtgcgtttgc cctgtcggcc gcccggcccg
     1381 acagctatgc tgcaagcgtg cggcgcaccg aagcgccgtc gccaatggcg atggcctggc
     1441 gaacccagcc ttcgagcaat gacaaggaga aacactcatg accgagtccg cagaatcgcc
     1501 gcatctgaag ccgaacacct acgaaatggc ccccgaggac aagggcgaaa agatcggtta
     1561 cgacgccgcg gatcccaagt tctccgacga gggctacacc gccgagggcg tgctacggca
     1621 ccgcaacgaa caggcaccct ggggtcgcac cgcagagggc gaaaagggcg atcgccctga
     1681 ccagtagcgg cgcgggcggc acggctatgt cgcgaatcga tgggctggcg ggcttcatct
     1741 ccgccagccc atcgtcattc cacgccgccc ggtcggccgc cgcggcactg gttgaggccg
     1801 gcttcaccga actcgccgag agcgacccgg tcagcggcga gccgggcggc tacgtcatgg
     1861 tgcgcgacgg ctcggtgctg gcctggcgtc ttcccgagcg gctggaccaa ccgtgcttcc
     1921 acctcgtcgg ggcgcacacc gattcgccgg gcttcgtgct caagccgcgg cccacctcgc
     1981 acaccctggg ctggcagcag gccggcatgg aggtatatgg cggcccgttg ccgaactcgt
     2041 ggcttgatcg cgagttcggc ctcgccggac gtctggtcac cgcgtccggc gagcaggtgc
     2101 tggtggcgac cggacccctg ctgcgcgtac cgcaggtggc accccacctc gaccgcagcg
     2161 tgcacgagaa gctgctgctc gaccggcaac accacctgca cccgatcttc gccgtctcgc
     2221 ggccggacgc cgacattctc gccgtcgtcg cgcagaccgc cggactgagg caggaggagg
     2281 tgtggggcca cgacctgtgc gccttcgtga ctgagccgcc cgcccggatc gggctggacg
     2341 gcgacctctt cgcctcagcc cggctcgaca atctggccag cgtgtatgcg ggcgtccgtg
     2401 cgctggcgga cgcgaccgac ctgcgccgga tcgccgtgct ggcctgtttc gatcacgaag
     2461 aggtcggcag cgcgagtgcg accggggcga gcgggccact gttggctacg ctgctgctcc
     2521 gcatcggcga cgccctgggg tggagcgtcc agaccgccgc cgctgcccgg gcccgctcgc
     2581 tgctggtgtc ggccgacgcc gggcatgcgg tccaccccaa ctatgcgcag ctgcacgacc
     2641 ctgatcaccg tccgctgctc aacgacggcc cgctgatcaa gttcaacgcg aaccagcgct
     2701 acgccaccga cgcggtgggg gaggccgcct ggcgccgcgc gtgcgccgag gctgggatac
     2761 cgactcagga cttcgtgtcg aacaacgccg tgccctgcgg atcgaccatc ggccccctgg
     2821 cggccacccg cctgggcatt cgcaccgtcg acgtcggcat accgctgttg agcatgcact
     2881 cggcacgcga actgtgcggc gtgcgcgacc cgggttggct agccgggggc ctgcacgcct
     2941 tctactgcgc gagttgagct gatctgtcgc cggttgagcc tcaaacctcg agagcgggtg
     3001 aggggtgacg gacggtcccc ccatcccttc tgcgtcggtc tcgacaagct cgaccaacgg
     3061 ggtgccggcc cgcctgggac gcccaggcca gcgggcactc agaccgcgac cggacgtccg
     3121 ccctggacgg ccatcgcacc ccgctcgacc cgaatggtga cccgtttcga ggtggacgtg
     3181 tgtgcagacc cgatcgaccg cgtcgcgcgg caccagcacg ttggcctcgg gaaagtacat
     3241 cgcggcgcag cccttgacgg tggggcaggc cccacccggt acgcccgcag cacccggtcg
     3301 ggttcagccg gccattcgct gaactcgtcg acgggctggc cgttcaccag ccccagttcg
     3361 acgatgtcgt cggcaggcat caggaccacg aaccgtccgt tgtgcacgcc gcggtaccac
     3421 tcgttgagcg agtggctcgt ggtgtggtgc tgatcggggg cgcccatcgt ctggtgcatc
     3481 ggatgggtga tccggccctg caggcccagc cagtactgac cattgtccag cctgctgatc
     3541 agcgaatttc tcggccgaga actcgtcctc gacgaccccc ggggtcgcct cccagacgat
     3601 ggtcttgatg ccgttgtccc agaactccac cttggagtga tcggggttgg cccccgcgca
     3661 gcaggggcag tcgaagcccc tgtggtggtt gatcgtcacc gcgagcgtcg ccgtcttcgc
     3721 cgcccgagat gctgatccat cggcagcatc gccttctcga ccgcctcgac gtcggcggcg
     3781 tagtccatcg gcgccttgat ctcgagctcg gtgtcggtga aatcctgctt cgggggatgg
     3841 tcgaagactc tggcgagagg gtcgaggggt ccggtcacgc gtgggcttct tccgggagtt
     3901 cgctcggcgc catgaccacg acacggtcgg cacggctgta gacgttcatg gactcgccgc
     3961 gcagaaaacc gaccagggtg aggccggcgg attccgcgag ctcgacggcc agcgacgacg
     4021 gggccgagat ggcggccagc gccggtatgc cggccatcac cgccttctgc accagttcga
     4081 agctggcccg acccgagacc tgcagcacag tgccggacaa cggcagccga tcggccagca
     4141 cggcccaacc gatcaccttg tcgacggcgt tgtgccggcc gacgtcctcg cgtagtacga
     4201 gcagttcgcc ggacgccgcg tcgaacaggg cggcggcgtg cagtccgccg gtcttgtcga
     4261 agacggcctg gtgccggcgc aacagggtgg gtatccgggt gagcgtctcg gcggtgacgc
     4321 acgtggcatc gttcgccacg tcgtagcagg aaaccgtccg gatggcgtcg atcgaggcct
     4381 tgccgcacac cccgcacgaa ctggtggtgt agaagttgcg gttcggctcg ggcggtgcga
     4441 cctcggcggc cagcgtcacg tcgagcacgt tgtaggtgtt gacctcaccg cccgagcctg
     4501 gaccgccgca gtgaatggcc gaacgaaagt caccgccccg ggcgatcaca ccttctgaca
     4561 ccagaaagcc cgcggccaac tcgacgtccg agcccggggt gcgcatcgtg acggccagag
     4621 ccctgccgcc gacgcgcaac tccaggggtt cttcgacggc cagcacgtcg acccgcctgg
     4681 tggccccgct cgtgagggcg atgctcacga cgggtctgcg cgccgtgatc cgccccatgg
     4741 cgtcatgcta gacccaccgt gaaggcggac cggacgcttc aacttcgctt tggatcaggc
     4801 gcgaaaaggc tgcgatggtg gcgagactgg tgttcgaaga ttggccgtcg gccacgccga
     4861 gtagtctcgt cgtagagcgc tgtgtaggtc gacggagcag cgttcgtgga aggagtcaaa
     4921 gcgtggattt tctcgacccg cttctgctgg ccaggtggca gttcggtctg acgactctct
     4981 accacttcat cttcgtgccg ctgaccctgg gcatgtcgct gtcggtggcg atctttcaga
     5041 ccgcatgggt gcgcaccggc aactacaagt ggctgagcct cacgcgcttc ttcggcaaga
     5101 tcttcctgat caacttcgcg atgggtgtgg tcaccggcat cgtgcaggag tttcagttcg
     5161 gcatgaactg gtcggcctac tcacgcttcg tgggtgacgt cttcggcgcc ccgctggcct
     5221 tcgaggggct gatggcgttc ttcttcgagg cgaccttcat cggcctgtgg atcttcggct
     5281 gggaccggct gcccaagaaa ctgcacctgg cgacgctgtg gatcgccacc ttcggcgcgt
     5341 gggcctcggc ctacttcatc ctggccgcca acgccttcat gcagaacccg gtcgggtacg
     5401 tgatggctca ggacggtcgc cgtgccgaga tggccgactt cggtgcactg ctgaccaacc
     5461 gcgtcgcgct ggcggctttt ccgcacacgc tgctggccag cctgatgttc gcggccggcg
     5521 tgatcatcgc ggtcgcggcg tggcacctcg cgaggcgtca acaccacgaa acgatgacca
     5581 ccgcgctgaa gtacggttgc tggctgatga tcggcgcctt tctgggcacc gcactgtccg
     5641 gtgaccagtt gagcctgcag atggtcgcca cccagccgat gaagatggcc gcggccgagg
     5701 cgctgttcga caccgcctgc ggcgccgacg cgtccttctc gatcttcacc ctcggcaccc
     5761 cggacggctc cggtgaggtg tggtccctgc gggtgccgta cctgctcgcg ctgctctcca
     5821 cccacgagtt cgcggggtgc gtcgagggca tcaacaacct caacgcgcag tacgcggcgc
     5881 agtggccgca gctggcagcc cacgtggacg gcgtgttcaa cccgattccg tggatcacgt
     5941 actggtcgtt ccgctggatg atgggcctcg gcctgatggc caccgccgtc gccgtcgtgg
     6001 gcctgtgggt gacccgcacc aaggccgcga aaccgccggc gcagtggatg tggaaggtgg
     6061 ccgtctggtc ggcgccgctg gcgctgctgg cgattctggt cggctggatc ttcaccgaga
     6121 tgggccggca gccctggatc gtgttcagcc tg
//
LOCUS       DAOSUG010000132         6100 bp    DNA     linear   ENV 10-JUN-2024
DEFINITION  MAG TPA_asm: Micropruina sp. isolate AMR_MDS_2195
            NODE_6016_length_6100_cov_3.916294, whole genome shotgun sequence.
ACCESSION   DAOSUG010000132 DAOSUG010000000
VERSION     DAOSUG010000132.1
DBLINK      BioProject: PRJNA925477
            BioSample: SAMN32864262
            Sequence Read Archive: SRR4236673
KEYWORDS    WGS; ENV; Metagenome Assembled Genome; MAG; Third Party Data; TPA;
            TPA:assembly.
SOURCE      Micropruina sp. (wastewater metagenome)
  ORGANISM  Micropruina sp.
            Bacteria; Bacillati; Actinomycetota; Actinomycetes;
            Propionibacteriales; Nocardioidaceae; Micropruina.
REFERENCE   1  (bases 1 to 6100)
  AUTHORS   Abdulkadir,N., Saraiva,J.P., Zhang,J., Stolte,S., Gillor,O.,
            Harms,H. and Rocha,U.
  TITLE     Genome-centric analyses of 165 metagenomes show that mobile genetic
            elements are crucial for the transmission of antimicrobial
            resistance genes to pathogens in activated sludge and wastewater
  JOURNAL   Microbiol Spectr 12 (3), e0291823 (2024)
   PUBMED   38289113
REFERENCE   2  (bases 1 to 6100)
  AUTHORS   Abdulkadir,N.
  TITLE     Direct Submission
  JOURNAL   Submitted (24-MAR-2023) Environmental Microbiology, Helmholtz
            Center for Environmental Research -UFZ, Permoserstr 15, Leipzig
            04318, Germany
COMMENT     The annotation was added by the NCBI Prokaryotic Genome Annotation
            Pipeline (PGAP). Information about PGAP can be found here:
            https://www.ncbi.nlm.nih.gov/genome/annotation_prok/
            
            ##Genome-Assembly-Data-START##
            Assembly Method        :: SPAdes v. 3.11.1
            Genome Representation  :: Full
            Expected Final Version :: Yes
            Genome Coverage        :: Not Applicable
            Sequencing Technology  :: Illumina MiSeq
            ##Genome-Assembly-Data-END##
            
            ##Genome-Annotation-Data-START##
            Annotation Provider               :: NCBI
            Annotation Date                   :: 05/04/2023 04:57:10
            Annotation Pipeline               :: NCBI Prokaryotic Genome
                                                 Annotation Pipeline (PGAP)
            Annotation Method                 :: Best-placed reference protein
                                                 set; GeneMarkS-2+
            Annotation Software revision      :: 6.5
            Features Annotated                :: Gene; CDS; rRNA; tRNA; ncRNA
            Genes (total)                     :: 2,776
            CDSs (total)                      :: 2,750
            Genes (coding)                    :: 2,714
            CDSs (with protein)               :: 2,714
            Genes (RNA)                       :: 26
            tRNAs                             :: 24
            ncRNAs                            :: 2
            Pseudo Genes (total)              :: 36
            CDSs (without protein)            :: 36
            Pseudo Genes (ambiguous residues) :: 0 of 36
            Pseudo Genes (frameshifted)       :: 2 of 36
            Pseudo Genes (incomplete)         :: 32 of 36
            Pseudo Genes (internal stop)      :: 2 of 36
            ##Genome-Annotation-Data-END##
FEATURES             Location/Qualifiers
     source          1..6100
                     /organism="Micropruina sp."
                     /mol_type="genomic DNA"
                     /submitter_seqid="NODE_6016_length_6100_cov_3.916294"
                     /isolate="AMR_MDS_2195"
                     /isolation_source="Activated sludge of municipal
                     wastewater treatment plants"
                     /db_xref="taxon:2737536"
                     /environmental_sample
                     /geo_loc_name="Canada"
                     /lat_lon="49.25 N 123.1 W"
                     /collection_date="2015-06-16"
                     /metagenome_source="wastewater metagenome"
                     /note="metagenomic"
     gene            complement(<1..206)
                     /locus_tag="PLF56_05750"
     CDS             complement(<1..206)
                     /locus_tag="PLF56_05750"
                     /inference="COORDINATES: ab initio
                     prediction:GeneMarkS-2+"
                     /note="Derived by automated computational analysis using
                     gene prediction method: GeneMarkS-2+."
                     /codon_start=1
                     /transl_table=11
                     /product="hypothetical protein"
                     /protein_id="HOQ53101.1"
                     /translation="MRLADLLPSVTAPPSNAPDAAPDPRASDPAGPTAVHPDAAPDPR
                     TSEPAGPTAAHPDAALDPDALYEA"
     gene            361..1182
                     /locus_tag="PLF56_05755"
     CDS             361..1182
                     /locus_tag="PLF56_05755"
                     /inference="COORDINATES: protein motif:HMM:NF013245.2"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_component: GO:0016020 - membrane [Evidence IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="ABC transporter permease"
                     /protein_id="HOQ53102.1"
                     /translation="MLGDAIRATRQLALINYQNWQRTYAMAQVALTKRYSGSALGVAW
                     AVIRPAIYIGAYWFAVSVGMRGGASAAVGAPYLLWLVPGNLAWFFMSDCLREAGESIR
                     KNSQFVTKMTYPIATLPVSEVLSHFLVHLVMMCLNIILVLVSGYGLTLAAIQLPYFML
                     CALVLSVVVSTLFSALTAISADIGHLIKSALQLLFWVTPVLWTASHMSSPFRYIIMSN
                     PLAYVIQGYRDSLVLNNWVTSYLPYTAYFWGLMGLLTLVSSLVFTKLAPEFADVL"
     gene            1192..2445
                     /locus_tag="PLF56_05760"
     CDS             1192..2445
                     /locus_tag="PLF56_05760"
                     /inference="COORDINATES: protein motif:HMM:NF012235.2"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_function: GO:0005524 - ATP binding [Evidence IEA];
                     GO_function: GO:0016887 - ATP hydrolysis activity
                     [Evidence IEA];
                     GO_function: GO:0042626 - ATPase-coupled transmembrane
                     transporter activity [Evidence IEA];
                     GO_function: GO:0140359 - ABC-type transporter activity
                     [Evidence IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="ABC transporter ATP-binding protein"
                     /protein_id="HOQ53103.1"
                     /translation="MPTNQLTAPTVVMDEFNLDGPAVEVEHLTKVYRTYSSSLRRAIA
                     PFRKGPTGKKFVALNNVSATLQRGEVLAVLGRNGSGKSTLSKIIAGVTTPTSGTLRVN
                     GRISAMLELTSGFDPQLTGVENIYLRALAMGIPREEAEERRPEIIAFADLGDHINQPV
                     RTYSSGMKSRLGFAVTTSIDPDILIIDEALSVGDEIFRLKCIERMGAIRERGSSIVFV
                     SHSLGTVKAFCTKAIWINRGVVQEDGDVGPVTQAYEEFLRKERSSLRDELREASDQDA
                     VLEKSDVVQINEVRLTNASGSRTTTFVHGEDIYLHLGYTVKRPQAQLTFTYTITNSED
                     VEVFASDRRSPMLALDPSPGTHQVRARLIKPPLLGGGFRLTGELWNNASGFFVTHSNG
                     RRFSIEQDDFVGTGIAAIECELTHE"
     gene            2438..5005
                     /locus_tag="PLF56_05765"
     CDS             2438..5005
                     /locus_tag="PLF56_05765"
                     /inference="COORDINATES: protein motif:HMM:NF015644.2"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="UPF0182 family protein"
                     /protein_id="HOQ53104.1"
                     /translation="MSSSIGAVAVADEEQAPARPPGRWRIFWRRMRSFGPVFGIIVGL
                     IAAGVVGIAWLYIWTDHEWYASVGEDPVFWTRYASIWAVWAVFTAINFVALLVASRSA
                     WTAAGAKGRFRAVTGVATFTVAALMAYTMSSNWMVFRLAVAQSPFGITDPQFGLDAGF
                     FVFTLPALELLVGWGFQIVLLAMAVHLTIVLISSRLDYTGRLAADWWEVRRVFWRLLA
                     ALMVVTAANYLLKIWQLSYSTSQTSFAGASYVDVNVQLPGLALMALACLLIAGLLLVT
                     AQRRTFKPVVWAFIGLVVLALLTSTLAPSVVERFWARPNEASLEGRYLARNVAMTRAA
                     YQLDQVATVDHPGDDSVPSGSQQKVRDELATAPVWTTSTVGQAFNQLQTIRPYYQLSA
                     TSTDRYLIDGRLQQVLVAAREISPGNLPSAGSTFVNRRLVYTHGYGMAISSASSTTKE
                     GFPQFLVGDVPAKVSSTVQDPSSLTITQPRVYFGPDQSDWVVVNAGLDEFDYPSGTAN
                     VKNRYAGGDGVSVGGPLNRLAWALRLRSIDLLVSGYVHEDSRIVLNRSVVTRATKIAP
                     WLTYDTPYPALVDGRITWILDGYTTSDHFPYAQPLPNSSPFANGTNYVRNSVKVTVDA
                     ETGATHFYAVGADPVRDAWGRIFPGVISQDAIPAALAAHLRVPTKLFTAQTQVFAKYH
                     VSDPSVFYSQEDAWRIPSDASGKTIGSQYVLLNSTGTGMGLDLLQPYVLPNRDDLVGW
                     LSASSEPASYGDKTVFALPKSRVTLGAAQVSARINQDPAIAQQLTLWNQPGSTVTFGS
                     MLVLPVQGSTVYLQPVFLKAQNSAITELVGVIAVNGSAVTLGSSLPDALGKAFPSKG"
     gene            5100..5879
                     /locus_tag="PLF56_05770"
     CDS             5100..5879
                     /locus_tag="PLF56_05770"
                     /inference="COORDINATES: protein motif:HMM:NF012472.2"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="aldo/keto reductase"
                     /protein_id="HOQ53105.1"
                     /translation="MSSAGVTEARLGELTVRRVGFGAMQLPGPGVFGPPRDHDEAIAV
                     LRRAVELGVNHIDTAQYYGPDVANRLIREALHPYPDELVLVSKVGSVRDASGGWVPAQ
                     RPEQLRSAVDDNLRTLGVERLGAVNLRLMGGHTLPSEQTVPMEDQLAELVALREEGKI
                     AGVGISTADLAQVEQAIEQAGIVCVQNAYSLVDRSDAEVLDLCKRRGIAYVPYFRSGR
                     RSRTRPGSPTSPPSGPWRNDWTSRRRRWAWPGCWPRGTRCC"
     gene            5834..5980
                     /locus_tag="PLF56_05775"
     CDS             5834..5980
                     /locus_tag="PLF56_05775"
                     /inference="COORDINATES: protein motif:HMM:NF012472.2"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="aldo/keto reductase"
                     /protein_id="HOQ53106.1"
                     /translation="MGLAWLLAQGDQVLLIPGTSRVAHLEQNLAAAQLHLSPADLAEL
                     EGNG"
BASE COUNT          962 a         2040 c         2071 g         1027 t
ORIGIN      
        1 aacgcctcat acagggcgtc ggggtcgagg gcggcgtccg ggtgggcggc ggtcgggccg
       61 gcgggttccg aggtcctggg gtcgggggcg gcgtccgggt ggacggcggt cgggccggcg
      121 gggtccgagg ccctggggtc gggggcggcg tccggcgcgt tcgacggagg tgcggtaacg
      181 gacggaagca ggtcggcgag tctcatcgct gcccatcgtc ccatctgcgg cgcgcacagg
      241 agcgtccgca ctctgagcga cggcgtgttc gtcagccgcg gctttgccag cgggggtaca
      301 gtccaatccc gctggccgat gcaacctccc gacgcagcag acggcagtag gaagtgacac
      361 atgctgggcg atgcgattcg cgccaccagg cagctggcac tcatcaacta tcagaactgg
      421 caacgcacct acgcgatggc gcaggtggcg ctgaccaagc ggtacagcgg gtcggccctc
      481 ggtgtggcct gggcggtgat ccgtccggcc atctacatcg gcgcgtactg gttcgccgtc
      541 tcggtcggca tgcgtggtgg cgcgagcgca gcggtcggcg ccccgtatct gctgtggctg
      601 gtgcccggca acctggcgtg gttcttcatg tcggactgcc tgcgcgaagc cggcgaatcg
      661 attcgcaaga acagccagtt cgtcaccaag atgacctacc cgatcgcgac cctgccggtc
      721 tcagaggtgc tgtcgcactt tctggtgcac ctggtgatga tgtgcctcaa catcattctg
      781 gtactggtca gcggctacgg cctgaccctt gccgcgatcc agctgcccta cttcatgctg
      841 tgcgccctgg tgctgagcgt ggtggtctcg accctgttct cggcactgac cgcgatcagc
      901 gccgacatcg gccacctgat caagtcggcg ctgcaactgc tgttctgggt gaccccggtg
      961 ctgtggacgg cgagccacat gagttcgccg tttcgctaca tcatcatgtc caacccgctg
     1021 gcctacgtca ttcagggcta ccgcgactcg ctggtgctga acaactgggt gacgtcgtac
     1081 ctgccctaca ccgcctactt ctgggggttg atgggactgc tcaccctggt gagcagcctg
     1141 gtgttcacca agctcgcccc cgagttcgcc gacgtcttgt aacgaggtct gatgcccacg
     1201 aaccagttga ccgcaccgac cgtggtgatg gacgagttca acctcgacgg cccggccgtc
     1261 gaggtcgagc acctgacgaa ggtctaccgc acctactcca gctcactgcg gcgcgcgatc
     1321 gcaccgtttc gcaagggccc gaccggcaag aagttcgtgg ccctcaacaa cgtgtcggcc
     1381 accctgcaac gcggcgaggt gctggcggtg ctgggccgca acggctccgg caagtcgacg
     1441 ctgtcgaaga tcatcgccgg cgtgaccacg ccgaccagcg gcaccctgcg ggtcaacgga
     1501 cggatctcgg cgatgctcga gctgaccagc ggcttcgacc cgcaactgac cggcgtcgag
     1561 aacatctacc tgcgcgccct ggcgatgggc attccgcgtg aagaggccga ggagcggcgt
     1621 cccgagatca tcgccttcgc cgacctcggc gaccacatca accaaccggt gcgcacctac
     1681 tcctcgggca tgaagtcccg gttgggcttc gcggtcacca ccagcatcga ccccgacatt
     1741 ctgatcatcg acgaggcgct gtcggtgggc gatgagatct tccggctcaa gtgcatcgaa
     1801 cgcatggggg ccatccgcga gcgcggctcg agcatcgtgt tcgtcagcca ctcgctgggc
     1861 acggtgaagg cgttctgcac caaggcgatc tggatcaacc gcggcgtggt gcaggaggac
     1921 ggtgacgtgg gcccggtcac ccaggcctac gaggagttcc tgcgcaagga acgatcgtcg
     1981 ttgcgcgacg agttgcgcga ggcgtccgat caggacgccg tactggagaa gtccgacgtg
     2041 gtgcagatca acgaggtcag gctgaccaac gcctcgggca gccgcaccac gaccttcgtg
     2101 cacggcgagg acatctatct gcacctgggg tacacggtga agcggcccca ggcgcagctc
     2161 accttcacct acacgatcac caacagcgag gacgtcgagg tgttcgcctc cgaccggcgc
     2221 agccccatgc tcgcgctcga ccccagcccc ggcacccacc aggtgcgggc acggctgatc
     2281 aagcccccgt tgctgggtgg cggcttccgg ttgaccggtg agctgtggaa caacgcgtcc
     2341 ggcttcttcg tcacccattc caacggacgc cggttcagca tcgagcagga cgacttcgtc
     2401 ggcaccggca tcgccgcgat cgagtgcgag ctgacccatg agtagttcca tcggggcggt
     2461 ggccgtcgcg gacgaggagc aggcgcctgc gcgcccgccc ggcagatggc gcatcttctg
     2521 gcgccgcatg cgcagcttcg gtccggtgtt cggcatcatc gtcggcctga tcgcggccgg
     2581 cgtggtcggc atcgcctggc tgtacatctg gaccgaccac gagtggtacg cgagtgtggg
     2641 cgaggacccg gtcttctgga cccggtacgc ctcgatctgg gcggtctggg ccgtcttcac
     2701 cgcgatcaac ttcgtggcgc tgctggtcgc cagccgaagc gcgtggacgg ccgccggcgc
     2761 gaagggacgt ttccgtgccg tcaccggggt ggccaccttc accgtcgccg ccctgatggc
     2821 ctacaccatg tcgagcaact ggatggtgtt tcggctggcg gtcgcccagt cgccgttcgg
     2881 catcaccgat ccacagttcg gtctcgacgc cggcttcttc gtgttcaccc tgccggccct
     2941 ggagttgctg gtcgggtggg gctttcagat cgtgctgctc gcgatggcgg tgcatctgac
     3001 gatcgtgctg atcagcagcc ggctcgacta caccggacgc ctcgcggccg actggtggga
     3061 ggtgcgccgg gtgttctggc ggctgctggc ggcgctgatg gtggtgaccg ccgccaacta
     3121 cctgctgaag atctggcagc tcagctactc gacctcgcag accagcttcg cgggtgcgtc
     3181 ctacgttgac gtcaacgttc agttgcccgg cctggcgctg atggcgctgg cgtgcctgct
     3241 gatcgcgggg ctgctgctgg tcaccgcgca acgccgcacc ttcaagccgg tggtgtgggc
     3301 cttcatcggc ctggtggtgc tggccttgct gacctcgacg ctcgccccgt ccgttgtcga
     3361 acgcttctgg gccaggccga acgaggcgtc gctcgagggc cggtatctgg cccgcaacgt
     3421 ggctatgacc agggccgcgt accagctcga tcaggtcgcc accgtggatc atcccggtga
     3481 cgacagcgtg ccgtccggct cgcagcagaa ggtgcgcgac gagctggcca ccgcaccggt
     3541 gtggaccacg tcgacggtcg gtcaggcgtt caaccagctg cagacgatcc gtccgtacta
     3601 tcaactgtcg gcgaccagca ccgaccggta cctgatcgac ggacggttgc agcaggtgct
     3661 ggtggccgcc cgtgagatct cgcccggcaa cctgccgtcg gcgggcagca ccttcgtgaa
     3721 ccggcggctg gtctacaccc acggttacgg catggcgatc agctcggcca gcagcaccac
     3781 caaggagggc tttccgcagt ttctggtcgg tgacgtgccc gcgaaagtca gttcgacggt
     3841 ccaggacccc tcctcgctga ccatcaccca gccgcgggtc tacttcggcc cggaccagtc
     3901 cgactgggtg gtggtgaacg cgggcctcga cgagttcgac tacccgagcg gcacggccaa
     3961 cgtgaagaac cgctacgccg ggggcgacgg ggtgagcgtg ggcggtccgc tcaaccggct
     4021 ggcctgggcg ctgaggctgc gctcgatcga cctgctggtg tcgggctacg tgcacgagga
     4081 cagccgcatt gtgctcaacc gcagcgtggt cacccgagcc acgaagatcg cgccctggct
     4141 gacctacgac accccctatc ccgcgctggt cgacgggcgg atcacctgga ttctcgacgg
     4201 ctacaccacc agcgaccact ttccctacgc gcagccgctg ccgaacagct cgccgttcgc
     4261 caacggcacc aactacgtgc gcaactcggt caaggtgacg gtcgacgccg agaccggcgc
     4321 aacacacttc tatgcggtcg gtgccgaccc ggtcagggac gcctgggggc ggatctttcc
     4381 aggcgtgatc agccaggacg cgatcccggc ggcgttggcg gcgcacctgc gggtaccgac
     4441 gaaactgttc accgcacaga cgcaggtctt cgcgaagtac cacgtcagcg acccgtccgt
     4501 gttctacagc caagaggacg cctggcgcat tccgtccgac gcgtccggca agacgatcgg
     4561 ttcgcagtac gtgctgctga acagcaccgg aaccggcatg ggcctcgacc tgctgcagcc
     4621 ctatgtgctg ccgaaccggg acgatctggt gggctggctc agcgcctcgt ccgaaccggc
     4681 gagttatggc gacaagaccg tgttcgcgct gcccaagagc cgggtgacgc tcggtgcggc
     4741 gcaggtcagc gcgcggatca accaggatcc tgccatcgcc cagcagctga ccctgtggaa
     4801 ccagccgggc agcacggtca cgttcggcag catgctggtg ctgccggtgc agggcagcac
     4861 ggtgtatctg caaccggtgt ttctcaaggc gcagaacagc gcgatcaccg agttggtcgg
     4921 tgtgatcgcg gtcaacggca gcgccgtcac cctggggtcg tcccttccgg acgcgctcgg
     4981 caaggcgttt ccgagcaaag gctgaccagg gtccgggttc aaacaaagac caacaggcgt
     5041 tgagggctga actggtctgg ccttcgaagc catcgtcaac acaggtgagg agtcgtggga
     5101 tgagcagtgc tggggtcacg gaggcgcgtt tgggcgagct gacggtacgg cgggtcggtt
     5161 tcggcgccat gcagttgccc gggccgggcg tgttcggccc gccccgcgat cacgacgagg
     5221 cgatcgccgt gctgcggcgc gcagtcgagt tgggcgtcaa ccacatcgac accgcgcagt
     5281 actacggtcc ggacgtcgcc aaccggctca tccgcgaggc cctgcacccc tacccggacg
     5341 agctggtgct ggtctcgaag gtggggtcgg tgcgggacgc ctccggggga tgggtgccgg
     5401 cgcaacgtcc ggagcagtta cgctcggcgg tcgacgacaa cctgcggacg ctcggggtcg
     5461 aacggctggg cgcggtcaac ctgcgcctga tgggcggaca caccctgccg agcgagcaga
     5521 ccgtgcccat ggaggatcag ctcgccgagc tggtcgccct gcgcgaggag ggcaagatcg
     5581 ccggcgtcgg catctcgacg gccgacctgg cccaggtcga gcaggcgatc gagcaggccg
     5641 gcatcgtctg cgtgcagaac gcgtacagcc tggtcgatcg ctccgacgcc gaggtgctcg
     5701 acctgtgcaa gcggcgaggc atcgcctacg tgccgtactt ccgctcgggt cggcgttccc
     5761 gaacacgccc agggtcaccg accagcccgc cgtccgggcc gtggcgcaac gactggacgt
     5821 cacgccggcg caggtgggcc tggcctggtt gttggcccag ggggaccagg tgctgctgat
     5881 cccgggcacc tcgcgggtgg cccacctgga gcagaacctg gcggcggcgc agttgcacct
     5941 gtccccggct gacctggccg agctggaagg caacggctga gctgccggcg atccttctcg
     6001 cgggatatcg ctggtcctga cccccggtca tccgggacgt cctcgccccc gtcatcccgg
     6061 cgcacgccgg gacctccacc ctgcgccaag atcccagagc
//
LOCUS       DAOSUG010000133         6077 bp    DNA     linear   ENV 10-JUN-2024
DEFINITION  MAG TPA_asm: Micropruina sp. isolate AMR_MDS_2195
            NODE_6044_length_6077_cov_4.312355, whole genome shotgun sequence.
ACCESSION   DAOSUG010000133 DAOSUG010000000
VERSION     DAOSUG010000133.1
DBLINK      BioProject: PRJNA925477
            BioSample: SAMN32864262
            Sequence Read Archive: SRR4236673
KEYWORDS    WGS; ENV; Metagenome Assembled Genome; MAG; Third Party Data; TPA;
            TPA:assembly.
SOURCE      Micropruina sp. (wastewater metagenome)
  ORGANISM  Micropruina sp.
            Bacteria; Bacillati; Actinomycetota; Actinomycetes;
            Propionibacteriales; Nocardioidaceae; Micropruina.
REFERENCE   1  (bases 1 to 6077)
  AUTHORS   Abdulkadir,N., Saraiva,J.P., Zhang,J., Stolte,S., Gillor,O.,
            Harms,H. and Rocha,U.
  TITLE     Genome-centric analyses of 165 metagenomes show that mobile genetic
            elements are crucial for the transmission of antimicrobial
            resistance genes to pathogens in activated sludge and wastewater
  JOURNAL   Microbiol Spectr 12 (3), e0291823 (2024)
   PUBMED   38289113
REFERENCE   2  (bases 1 to 6077)
  AUTHORS   Abdulkadir,N.
  TITLE     Direct Submission
  JOURNAL   Submitted (24-MAR-2023) Environmental Microbiology, Helmholtz
            Center for Environmental Research -UFZ, Permoserstr 15, Leipzig
            04318, Germany
COMMENT     The annotation was added by the NCBI Prokaryotic Genome Annotation
            Pipeline (PGAP). Information about PGAP can be found here:
            https://www.ncbi.nlm.nih.gov/genome/annotation_prok/
            
            ##Genome-Assembly-Data-START##
            Assembly Method        :: SPAdes v. 3.11.1
            Genome Representation  :: Full
            Expected Final Version :: Yes
            Genome Coverage        :: Not Applicable
            Sequencing Technology  :: Illumina MiSeq
            ##Genome-Assembly-Data-END##
            
            ##Genome-Annotation-Data-START##
            Annotation Provider               :: NCBI
            Annotation Date                   :: 05/04/2023 04:57:10
            Annotation Pipeline               :: NCBI Prokaryotic Genome
                                                 Annotation Pipeline (PGAP)
            Annotation Method                 :: Best-placed reference protein
                                                 set; GeneMarkS-2+
            Annotation Software revision      :: 6.5
            Features Annotated                :: Gene; CDS; rRNA; tRNA; ncRNA
            Genes (total)                     :: 2,776
            CDSs (total)                      :: 2,750
            Genes (coding)                    :: 2,714
            CDSs (with protein)               :: 2,714
            Genes (RNA)                       :: 26
            tRNAs                             :: 24
            ncRNAs                            :: 2
            Pseudo Genes (total)              :: 36
            CDSs (without protein)            :: 36
            Pseudo Genes (ambiguous residues) :: 0 of 36
            Pseudo Genes (frameshifted)       :: 2 of 36
            Pseudo Genes (incomplete)         :: 32 of 36
            Pseudo Genes (internal stop)      :: 2 of 36
            ##Genome-Annotation-Data-END##
FEATURES             Location/Qualifiers
     source          1..6077
                     /organism="Micropruina sp."
                     /mol_type="genomic DNA"
                     /submitter_seqid="NODE_6044_length_6077_cov_4.312355"
                     /isolate="AMR_MDS_2195"
                     /isolation_source="Activated sludge of municipal
                     wastewater treatment plants"
                     /db_xref="taxon:2737536"
                     /environmental_sample
                     /geo_loc_name="Canada"
                     /lat_lon="49.25 N 123.1 W"
                     /collection_date="2015-06-16"
                     /metagenome_source="wastewater metagenome"
                     /note="metagenomic"
     gene            <1..605
                     /locus_tag="PLF56_05780"
     CDS             <1..605
                     /locus_tag="PLF56_05780"
                     /inference="COORDINATES: protein motif:HMM:NF024928.2"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=3
                     /transl_table=11
                     /product="ATP-binding domain-containing protein"
                     /protein_id="HOQ53107.1"
                     /translation="DDPQQALRARVVHQGRGIHDAAVRGDAERALHLLERHRLLCAHR
                     HGPHGVTTWARQAEFWLRASVPGFGAEGEWYAGRSVMVTANQPDWGLYNGDTGVVLTT
                     SGRPLVHFAGVAARPLSPELLGPIQSVDALTVHKSQGSQFSVVSLVLPSVDSPLLTRE
                     LLYTAVTRARDQVHLIGTPEALTQAVQRTARRASGLRDRL"
     gene            complement(657..2018)
                     /locus_tag="PLF56_05785"
     CDS             complement(657..2018)
                     /locus_tag="PLF56_05785"
                     /EC_number="3.2.1.86"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_015069493.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_function: GO:0008706 - 6-phospho-beta-glucosidase
                     activity [Evidence IEA];
                     GO_function: GO:0046872 - metal ion binding [Evidence
                     IEA];
                     GO_process: GO:0005975 - carbohydrate metabolic process
                     [Evidence IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="6-phospho-beta-glucosidase"
                     /protein_id="HOQ53108.1"
                     /translation="MKLTILGGGGFRTPFVWQALLRDRGEPRITEVALHDLDGSRLAT
                     MTLILRQFAQHYPDAPALSAHTELEAAVEGADFVFAAVRVGGNEGRCSDERVALDLDV
                     LGQETTGPGGIAYALRTLPVMLRVAEVVQGLAPHAYLLNFTNPAGIITEALQSVLGDR
                     VVGICDTPSGLGRRVAAALDLDPDTVQLDYLGLNHLGWLRRVYAGGVDVLPGLLADDA
                     RLARLEEAEVFGVEWLRELGLIPNEYLYYYYCNRDAVRAIRRASETRGQALLRTQQAF
                     FEAAAGAGSGASELWQRAVREREAGYLAEARDETRTAERPTDPVLDPAQLGYAGVALG
                     VMTAISRNEPRTMILNVRNAGTITALPSDAVVEVPTLVDGNGMHPLALSEQPDLHQLG
                     LMQQVKAVERHAIAAALTGSRREAMLAFALHPLVGSVRTARALVQGYAAADPALAARL
                     TGD"
     gene            complement(2088..2531)
                     /gene="arfB"
                     /locus_tag="PLF56_05790"
     CDS             complement(2088..2531)
                     /gene="arfB"
                     /locus_tag="PLF56_05790"
                     /EC_number="3.1.1.29"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_008363515.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_function: GO:0003747 - translation release factor
                     activity [Evidence IEA];
                     GO_process: GO:0006415 - translational termination
                     [Evidence IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="alternative ribosome rescue aminoacyl-tRNA
                     hydrolase ArfB"
                     /protein_id="HOQ53109.1"
                     /translation="MSELRIPPGPGIPAGLVIPDRELIERYSHASGPGGQGVNTTDSR
                     VQLSFDVAASEVLDQLQRERLLRRLSTRLDGTVLTVVAAERRSQLQNRGIARERLALL
                     LRDALAPPAPPRRASRPTRGSIERRLAGKRRRSELKRQRARPHEA"
     gene            complement(2573..3499)
                     /locus_tag="PLF56_05795"
     CDS             complement(2573..3499)
                     /locus_tag="PLF56_05795"
                     /inference="COORDINATES: ab initio
                     prediction:GeneMarkS-2+"
                     /note="Derived by automated computational analysis using
                     gene prediction method: GeneMarkS-2+."
                     /codon_start=1
                     /transl_table=11
                     /product="hypothetical protein"
                     /protein_id="HOQ53110.1"
                     /translation="MNRFRRIVVGVAAAALVLTGCSAAVEPVPSDWRLNVHQPGKWWT
                     QPLIPRSIVMQRCPPRDGYSSTPDLTSRALPPGTVIEFSYWKSHDHCDIGWSNPVSEV
                     SVTAREFATEQGLRRLCSSAGLPMDEGWHYLGSNTIERQVGYDDNRSTARFTAVAFTD
                     EFQTVVGCAAAYYDNPEANDQEDGWKNFYRTVQLSVGADQPGPASAPACPVQPSDLTL
                     SETGTLQAYDLRGAGAVRGTDGRVLTDATTVVVGLVGDTVTTTHPVVDGVAIVNASVE
                     PKAQITPQEKRPLSVEGTVYAADGRVLATCRG"
     gene            complement(3502..4434)
                     /locus_tag="PLF56_05800"
     CDS             complement(3502..4434)
                     /locus_tag="PLF56_05800"
                     /inference="COORDINATES: protein motif:HMM:NF012472.2"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="aldo/keto reductase"
                     /protein_id="HOQ53111.1"
                     /translation="MKTFFMPGAALTVPAVVAGMMRIEEKPDDQIRALFDAALAAGIN
                     FFDHADIYGTSVHACERRFADVLRLSPAQRGEIILQSKAGIVRDGPYFDFSYEHLIET
                     VEGSLRALKTDYLDVLLLHRPDALVEPDEVARAFDALESAGKVRAFGVSNHTPGQIEL
                     LRRSVRQALVANQVQLSITHAPIVAQGVAMNMAGLQQSISRDLGVLDYCRLNDITVQA
                     WSPFQAGVSAGVFLGSPDFAELNAVIDRLAASYDVPAEAIAVAWITRHPAHTQVVLGT
                     TSPERVTASARGADVTLTRPEWYELFRAAGYRVP"
     gene            4609..5916
                     /gene="ribA"
                     /locus_tag="PLF56_05805"
     CDS             4609..5916
                     /gene="ribA"
                     /locus_tag="PLF56_05805"
                     /EC_number="3.5.4.25"
                     /inference="COORDINATES: protein motif:HMM:NF001591.0"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_function: GO:0003935 - GTP cyclohydrolase II activity
                     [Evidence IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="GTP cyclohydrolase II"
                     /protein_id="HOQ53112.1"
                     /translation="MVERVTESVLPTRHGVFRIVGYRGADGIELVSLSQGIADDSPHS
                     VAPLVRLHSECLTGDALGSRRCDCGEQLDAALALIAAHGEGVLVYVRGHEGRGIGLVE
                     KLRAYRLQDQGFDTVDANLRLGHPADARDYGQAADILRDLGLQRIRLVSSNPAKQQAL
                     ALLGIDVVERVGAFVPVRPENARYLLTKRQRMRHDEPHESDGLQRLIDADALVDAPAP
                     LDPYGWLTGHDTWVVAQSAQSLDGFLATRAGEGAGLSGSSDHRHLHRLRSFSDAVVVG
                     AQTVVNDDPRLTVRLVPGTHPVRVVLDPHGRIPPAAKVLTSPDAPTLWLTGPDAPASP
                     TAEHVQQIRLGPGPWAPDSVLKVLRERGLNRVLIEGGGRTVSEFLAARALDRLYLTTV
                     PVLLGDGVPGVRVPPVEHIADAPRWPSQRFGLGDDVCTELTLR"
BASE COUNT          897 a         2153 c         2090 g          937 t
ORIGIN      
        1 cggacgatcc ccagcaggca ctgcgagccc gggtcgtcca tcagggccgc ggcatccacg
       61 acgccgcggt ccggggcgat gccgagcgag cgctgcacct gctcgagcgg catcggttgc
      121 tgtgtgcgca ccggcacggt ccgcacggcg tcaccacctg ggcccggcag gccgagttct
      181 ggttgcgcgc gtcggtgccc gggttcggcg ccgagggcga gtggtacgcc ggtcgttcgg
      241 tgatggtcac cgcgaaccag cccgactggg gcctctacaa cggcgacacc ggggtggtgc
      301 tcaccacgag cggacgaccg ctggtgcact tcgccggtgt cgctgcccgg ccgctcagcc
      361 ctgagctgct gggcccgatc caatcggtgg acgcgctcac cgtgcacaag tcgcagggca
      421 gccagttctc ggtggtctcg ctggtactgc cgagtgtcga ctccccgctg ctcacccgcg
      481 agctgctgta caccgcggtc acccgggccc gcgaccaggt gcacctgatc ggcacccccg
      541 aggcactcac gcaggccgtg cagcgcaccg cgcgccgggc ctcgggcctg cgcgaccggt
      601 tgtagccgcg atgcgacacg gtgccgccgg ttgccggggc cagcccaggc gcccgctcag
      661 tcgccggtga gcctcgccgc gagcgcggga tccgccgccg catagccctg taccagtgcc
      721 cgggccgtcc gcaccgagcc gaccagtgga tgcagcgcga acgccagcat cgcttcacgc
      781 cgtgaaccgg tgagggccgc tgcgatggca tggcgttcca cggccttcac ctgctgcatc
      841 aggcccaact gatgcaggtc gggttgttcg ctcagggcca acggatgcat gccgttgccg
      901 tccacgagcg tgggcacctc gacaacggca tccgagggta gagcggtgat cgtgccggcg
      961 ttgcgcacgt tgagaatcat cgtgcgcggt tcgttgcggc tgatggcggt catcacgccg
     1021 agggcgacgc cggcgtagcc gagctgggcc gggtcgagca ccgggtcggt gggccgctcg
     1081 gcagtacggg tctcgtccct ggcctcggcc aggtagcccg cctcgcgttc acgcaccgcc
     1141 cgctgccaca gctcggacgc acccgagccg gccccggcgg ccgcctcgaa gaacgcctgc
     1201 tgggtacgca gcaacgcctg cccccgggtt tcggacgccc gccgaatcgc ccggacggcg
     1261 tcgcggttgc agtagtagta atacaggtac tcattcggta tcaggcccag ctcgcgaagc
     1321 cattcgacgc cgaacacctc ggcctcctcg agccgggcca gccgcgcgtc atcggccagc
     1381 agcccgggca gcacgtccac accgccggcg tacacgcgcc gaagccagcc cagatgattc
     1441 aggccgaggt agtcgagttg caccgtgtcg gggtcgaggt cgagggcggc ggccacccgc
     1501 cgtcccagcc cggacggggt gtcgcagatg cccaccaccc gatcgcccag cacggactgc
     1561 agcgcctcgg tgatgatgcc cgccggattg gtgaagttga gcaggtaggc atgcggggcc
     1621 agcccctgca ccacctcggc cacgcgcagc atcaccggca gggtgcgcaa cgcgtaggcg
     1681 atgccgccgg ggccggtcgt ctcctgcccc agcacgtcca ggtcgagggc cacccgctcg
     1741 tcgctgcagc ggccctcgtt gccaccgacc cgcacggccg cgaacacgaa gtccgcgccc
     1801 tcgactgcag cctccagctc ggtgtgcgcg ctgagcgccg gtgcgtcggg gtagtgctgc
     1861 gcgaactgcc gcaggatcag cgtcatggtc gccaggcgcg agccgtccag gtcgtgcaga
     1921 gccacctcgg tgatccgcgg ctcaccccga tcgcgcagca gcgcctgcca cacgaacggg
     1981 gtgcgaaacc cgccgccacc cagaatcgtc aacttcatcg caatcccttc acccgtaccg
     2041 cccaccatca tggtcgacag ccaccacacc gggtggacgg cccaccgtca ggcttcgtgc
     2101 ggacgggccc gctgccgctt caactcgctt cgtcgtcgct tgcccgccag ccggcgctcg
     2161 atcgaacccc tggtcggacg cgaggcccgc cggggcggag ccggtggtgc gagcgcgtcg
     2221 cgcagcagca gcgccaggcg ctcgcgggcg atcccacgat tctgcagctg gctacgcctt
     2281 tcggcggcga ccacggtgag cacggtaccg tccagccggg tgctcaggcg gcgcaacagc
     2341 cgttcgcgct gcagctggtc cagtacctcg gacgcggcca cgtcgaacga caactgcacc
     2401 cgcgagtcgg tggtgttgac gccctgcccg cccgggcccg aggcgtgcga gtagcgctcg
     2461 atcagttcgc gatcggggat caccaacccg gccggtatgc ccggcccggg cgggatccgc
     2521 aactcgctca ccgggccacg gtagacgctg ccatcagcga ggcccgcatg gctcaccccc
     2581 ggcaggtcgc cagcacgcgg ccgtccgcgg cgtagaccgt gccttcgacg ctgagcggtc
     2641 gcttctcctg gggggtgatc tgggccttcg gctcgacgct ggcattgacg atcgcgaccc
     2701 cgtccacgac cgggtgggtg gtcgtcaccg tgtcgcccac caagccgacc acgacggtgg
     2761 ttgcgtcggt gagcacccgg ccgtcggtgc cgcggaccgc tcccgcgccc cgcaagtcgt
     2821 aggcctgcag agtgccggtc tccgagagcg tgaggtcact cggctgcacc gggcaggccg
     2881 gcgccgaggc tggtccgggt tggtcggctc cgacggagag ctgcaccgtt cggtagaagt
     2941 tcttccatcc gtcctcctgg tcgttcgcct cgggattgtc gtagtacgcc gccgcgcacc
     3001 cgaccaccgt ctgaaactcg tcggtgaagg cgacggcggt gaaccgtgcg gtcgagcggt
     3061 tgtcgtcgta cccgacctgc cgctcgatcg tgttgctgcc gaggtagtgc cacccctcat
     3121 ccatcggcag gccggcgctg gagcagaggc ggcgaagccc ttgctcggtg gcgaactccc
     3181 gggccgtcac tgacacctcg ctgaccgggt tcgaccagcc gatgtcgcag tgatcgtgcg
     3241 atttccagta ggagaactcg atgacggtgc ccggcggcag cgcccggctg gtgagatctg
     3301 gcgtggacga atagccgtcg cggggtgggc atcgttgcat cacgatcgat cgcggaatca
     3361 gcggttgggt ccaccatttg cccggctgat gtacgttcaa ccgccagtcg gacggaacgg
     3421 gctcgaccgc agcggagcag ccggtcagca ccagggcagc ggctgcgaca cccacgacga
     3481 ttcgccggaa ccggttcatc ctcaaggcac ccgataaccg gctgcccgga acagttcgta
     3541 ccactccgga cgggtcaacg tgacgtccgc accgcgcgcc gatgccgtca cccgctcagg
     3601 gctcgtggtg cccagcacca cctgcgtgtg ggccggatgc ctggtgatcc aggccaccgc
     3661 aatggcctcg gccggcacgt cgtagctggc ggccaaccgg tctatcactg cgttcagctc
     3721 agcgaagtcc ggcgacccga ggaacacccc cgccgacacg ccggcctgga acggcgacca
     3781 ggcctgcacg gtgatgtcgt tcagccggca gtagtccagc acgcccagat cgcggctgat
     3841 cgactgctgc agcccggcca tgttcatcgc caccccctgc gccacgatcg gtgcatgcgt
     3901 gatcgacagt tgcacctggt tggccaccag tgcctgtcga acggaacgcc tgagcagttc
     3961 gatctggccc ggggtgtggt tcgacacccc gaaggcgcgc accttgccgg ccgactcgag
     4021 ggcgtcgaag gcgcgcgcca cctcgtccgg ctccacgagg gcgtccggac gatgcagcaa
     4081 cagcacgtcc agatagtcgg tcttcagggc ccgcagcgac ccctccaccg tctcgatcag
     4141 atgctcgtag gagaagtcga agtacggtcc gtcccgaacg atgccggcct tgctctgcag
     4201 gatgatctcg ccccgttgcg ccggactcag tcgcagtacg tcggcgaacc gcctctcgca
     4261 cgcgtgcacc gaggtgccgt agatgtcggc gtgatcgaag aagttgatac ccgccgccag
     4321 ggctgcgtcg aacagggccc ggatctggtc gtcaggcttt tcctcgatgc gcatcatgcc
     4381 ggcgaccacc gccggcacgg tcagtgcggc gccaggcatg aaaaaggtct tcatgcgggc
     4441 cctcctcgtc ttgtcggtgg tgctcgcccg cccctatggg tgggttcgcg ggtagtggga
     4501 tgcctcgtac catgtcacga acagctccag tcaccaaggt gaggggccgg aagcgtcccc
     4561 gagtgaaaca ggtccgatat cagcgctgag caaacgacac ccgccctcct ggtcgagcgg
     4621 gtcacggagt cggtgctccc gacccggcac ggagtgtttc gcatcgtcgg ctaccgcggt
     4681 gccgacggta tcgaactggt cagcctgtcc cagggcatcg cggacgatag tccgcactcg
     4741 gtcgcgccat tggtgcggct gcattccgaa tgcctcaccg gcgatgcgct gggctcgcgg
     4801 cggtgcgact gcggcgaaca gttggacgct gccctggccc tgatcgccgc gcacggcgag
     4861 ggggtgctgg tctacgtccg tgggcacgag ggccggggca tcgggctggt cgagaagctg
     4921 cgcgcctacc ggctgcagga tcaggggttc gacaccgtgg acgccaacct gcgcctgggc
     4981 catcccgccg atgcccgcga ctacggccag gcggccgaca ttctgcgcga cctgggcctg
     5041 cagcggatcc ggctggtctc gtccaacccg gccaagcagc aggcgctggc gttgctcggc
     5101 atcgatgtcg tcgaacgggt cggcgccttc gtgccggtac ggcccgagaa tgcccgctat
     5161 ctgctcacca agcggcagcg catgcgccat gacgagccac acgagagcga cggcctgcag
     5221 cggctgatcg atgccgacgc actcgtggac gcccctgcac cgttggatcc ctacggctgg
     5281 ctgaccggtc acgacacctg ggtggtcgcc cagagcgccc agagcctcga cggcttcctg
     5341 gccacgcgtg caggtgaggg cgcgggcctg tccgggtcgt ccgatcaccg gcacctgcat
     5401 cggctgcgct cgttcagtga cgccgtggtg gtgggggcgc agaccgtcgt caacgacgac
     5461 ccgcggctca ccgtgcggct ggtgccgggc acgcaccccg tccgggtggt gctcgacccc
     5521 catggtcgca ttccaccggc cgcgaaggtg ctcacctcac cggacgcgcc gacgctgtgg
     5581 ctgaccggcc cggacgcacc cgcgtcaccg acggccgagc acgtgcagca gattcgcctc
     5641 ggcccgggcc cgtgggctcc tgattcggtg ctgaaggttc tgcgtgagcg tgggctgaac
     5701 cgggtgctga tcgaaggggg tggacgcacc gtgtcggagt ttcttgctgc ccgagccctc
     5761 gatcggctct atctgaccac cgtgccggtg ctgttgggcg acggtgtgcc cggcgtccgg
     5821 gtgccgccgg tcgagcacat cgccgacgcg ccccgctggc ccagtcaacg attcggcctg
     5881 ggcgacgacg tctgtacgga gttgaccctg cgctgaggcg ccgggtggga ttcgaccagc
     5941 ggtaggggcg acaggctcgt ccggcggtgg gtcacgaccg ggcggtagtg ggtttcggac
     6001 aggctcgtcc agcggtaggt cacgaccggg cggtagtggg tttcggacag gctcgtccag
     6061 cggtaggtca cgaccgg
//
LOCUS       DAOSUG010000134         6044 bp    DNA     linear   ENV 10-JUN-2024
DEFINITION  MAG TPA_asm: Micropruina sp. isolate AMR_MDS_2195
            NODE_6081_length_6044_cov_4.335281, whole genome shotgun sequence.
ACCESSION   DAOSUG010000134 DAOSUG010000000
VERSION     DAOSUG010000134.1
DBLINK      BioProject: PRJNA925477
            BioSample: SAMN32864262
            Sequence Read Archive: SRR4236673
KEYWORDS    WGS; ENV; Metagenome Assembled Genome; MAG; Third Party Data; TPA;
            TPA:assembly.
SOURCE      Micropruina sp. (wastewater metagenome)
  ORGANISM  Micropruina sp.
            Bacteria; Bacillati; Actinomycetota; Actinomycetes;
            Propionibacteriales; Nocardioidaceae; Micropruina.
REFERENCE   1  (bases 1 to 6044)
  AUTHORS   Abdulkadir,N., Saraiva,J.P., Zhang,J., Stolte,S., Gillor,O.,
            Harms,H. and Rocha,U.
  TITLE     Genome-centric analyses of 165 metagenomes show that mobile genetic
            elements are crucial for the transmission of antimicrobial
            resistance genes to pathogens in activated sludge and wastewater
  JOURNAL   Microbiol Spectr 12 (3), e0291823 (2024)
   PUBMED   38289113
REFERENCE   2  (bases 1 to 6044)
  AUTHORS   Abdulkadir,N.
  TITLE     Direct Submission
  JOURNAL   Submitted (24-MAR-2023) Environmental Microbiology, Helmholtz
            Center for Environmental Research -UFZ, Permoserstr 15, Leipzig
            04318, Germany
COMMENT     The annotation was added by the NCBI Prokaryotic Genome Annotation
            Pipeline (PGAP). Information about PGAP can be found here:
            https://www.ncbi.nlm.nih.gov/genome/annotation_prok/
            
            ##Genome-Assembly-Data-START##
            Assembly Method        :: SPAdes v. 3.11.1
            Genome Representation  :: Full
            Expected Final Version :: Yes
            Genome Coverage        :: Not Applicable
            Sequencing Technology  :: Illumina MiSeq
            ##Genome-Assembly-Data-END##
            
            ##Genome-Annotation-Data-START##
            Annotation Provider               :: NCBI
            Annotation Date                   :: 05/04/2023 04:57:10
            Annotation Pipeline               :: NCBI Prokaryotic Genome
                                                 Annotation Pipeline (PGAP)
            Annotation Method                 :: Best-placed reference protein
                                                 set; GeneMarkS-2+
            Annotation Software revision      :: 6.5
            Features Annotated                :: Gene; CDS; rRNA; tRNA; ncRNA
            Genes (total)                     :: 2,776
            CDSs (total)                      :: 2,750
            Genes (coding)                    :: 2,714
            CDSs (with protein)               :: 2,714
            Genes (RNA)                       :: 26
            tRNAs                             :: 24
            ncRNAs                            :: 2
            Pseudo Genes (total)              :: 36
            CDSs (without protein)            :: 36
            Pseudo Genes (ambiguous residues) :: 0 of 36
            Pseudo Genes (frameshifted)       :: 2 of 36
            Pseudo Genes (incomplete)         :: 32 of 36
            Pseudo Genes (internal stop)      :: 2 of 36
            ##Genome-Annotation-Data-END##
FEATURES             Location/Qualifiers
     source          1..6044
                     /organism="Micropruina sp."
                     /mol_type="genomic DNA"
                     /submitter_seqid="NODE_6081_length_6044_cov_4.335281"
                     /isolate="AMR_MDS_2195"
                     /isolation_source="Activated sludge of municipal
                     wastewater treatment plants"
                     /db_xref="taxon:2737536"
                     /environmental_sample
                     /geo_loc_name="Canada"
                     /lat_lon="49.25 N 123.1 W"
                     /collection_date="2015-06-16"
                     /metagenome_source="wastewater metagenome"
                     /note="metagenomic"
     gene            624..1775
                     /locus_tag="PLF56_05810"
     CDS             624..1775
                     /locus_tag="PLF56_05810"
                     /inference="COORDINATES: protein motif:HMM:NF025910.2"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="tetratricopeptide repeat protein"
                     /protein_id="HOQ53113.1"
                     /translation="MAPKANEPATPVDVDLSLLSRGVKAELRSLTVEHAEVVAAHLAA
                     AAELIDTDPELAYAHAEAARRRAARLPVVREAAGTTAYAAGEFAAALNEFRALRRMTG
                     TDDFLAVMADCERALGRPAAALKLVKEGLAAQPPIASRVELKLVEAGARDALGQRDEA
                     VRLLRHEIELLGARGPKNARARLRYFFAQLLAEAGSVDEAEKWFASAVRLDPDDETGA
                     AEALAELQGMSIEFDDTADDLYDEPAQAAPVSTAPGSSAQQLDRGEYSGAQAAPATAA
                     DQSAVSEGEHEDSSQSSVDPSFEQPSIGTFDEPEERQELPVDFSVEQPSNPTQGEPEE
                     RSEAPVDSSVEQPPNPTSGEPEDRSEAPVDPSLEQPSNPTSGEAEENDR"
     gene            1772..2764
                     /locus_tag="PLF56_05815"
     CDS             1772..2764
                     /locus_tag="PLF56_05815"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_002548744.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_function: GO:0016787 - hydrolase activity [Evidence
                     IEA];
                     GO_process: GO:0008152 - metabolic process [Evidence IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="HAD-IIA family hydrolase"
                     /protein_id="HOQ53114.1"
                     /translation="MSARIIDDHDVAFFDLDGVIYLGPVAVPGAVEGITRIRETGARV
                     MYVTNNAARSADAVAHHLSALGFEAGPDDLITSAQVAVDVLAERLPAGSLLLVAGTRN
                     LIELAQAAGFRTTASADDRPDAVLQGYDPLIEWPVLDEAAIAVQRGATWYGTNDDLNR
                     PTNRGLVPGLGAQLAVVGSTVRQQPVVFGKPHAPMLDYAVTHTAAQRPIFVGDRIDTD
                     IMGANRAGMPSLLVFTGAHGKRDLLEAGPGDRPSYIGADLRALLEPPRTAAQHGHAWH
                     CGDAVAELLEGEASVRGELSTQAGQFDALWALANLVWATDADALAALALLDQVP"
     gene            2900..3484
                     /locus_tag="PLF56_05820"
     CDS             2900..3484
                     /locus_tag="PLF56_05820"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_008358390.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="biotin transporter BioY"
                     /protein_id="HOQ53115.1"
                     /translation="MPTGTRSSTADLAYIAVFAALIAVFSLVPGVPIGPVPITLQTLG
                     VALAGLVLGPWRGFAATALYLLVGFAGLPVFAGGASGLGVLGKPSIGYLLSFPFAALV
                     AGLLARWLGGRNPRLRFLWLFLAGLGASFLIVHPAGIAGLMLILHIDFAKALAIDLAF
                     WPGDVAKNLVAAAIALAVHKAFPALLAQRVPVAA"
     gene            3502..4194
                     /locus_tag="PLF56_05825"
     CDS             3502..4194
                     /locus_tag="PLF56_05825"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_014846124.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_function: GO:0005524 - ATP binding [Evidence IEA];
                     GO_function: GO:0016887 - ATP hydrolysis activity
                     [Evidence IEA];
                     GO_function: GO:0042626 - ATPase-coupled transmembrane
                     transporter activity [Evidence IEA];
                     GO_function: GO:0140359 - ABC-type transporter activity
                     [Evidence IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="ABC transporter ATP-binding protein"
                     /protein_id="HOQ53116.1"
                     /translation="MIRLSAVSVRVPSLAPPGFKLLLDEVHLDLAEHRIALIGANGSG
                     KSTLLRLLNGLLLPTQGRVLVDGLDTRSDAAAVLRRVGFTFTDPLSQLVLPTPLDDVQ
                     LSLRARVRNRTERQAAARDWLNRYGLAEVAEHSIYDLSGGERQLAALVSVLAVEPSVL
                     VCDEPTTLLDLRNRNLVAGVLDALPHQVILATHDLELAAQAERVVLIAGGRVAADGSP
                     FEVIDTYRRSMA"
     gene            4191..4802
                     /locus_tag="PLF56_05830"
     CDS             4191..4802
                     /locus_tag="PLF56_05830"
                     /inference="COORDINATES: ab initio
                     prediction:GeneMarkS-2+"
                     /note="Derived by automated computational analysis using
                     gene prediction method: GeneMarkS-2+."
                     /codon_start=1
                     /transl_table=11
                     /product="energy-coupling factor transporter transmembrane
                     protein EcfT"
                     /protein_id="HOQ53117.1"
                     /translation="MRSTDALFGNYLPGTSVWHRAGVGWKYLVVLVLTLPALIVQQPW
                     ATTVALVITVLGLLSTGLPARATLALPWGLIGLLAVLALFQVLVGQPTLALVVPGNVL
                     LAVLASRLLITTTRAAVLIDALVAAADRIPFVDGERFGLSIGIMLRSVPFLTGAFYDV
                     RDAARARGLERHWIARLTPVVVRAVGFAQTTGDALAARGLGER"
     gene            4884..5057
                     /locus_tag="PLF56_05835"
     CDS             4884..5057
                     /locus_tag="PLF56_05835"
                     /inference="COORDINATES: ab initio
                     prediction:GeneMarkS-2+"
                     /note="Derived by automated computational analysis using
                     gene prediction method: GeneMarkS-2+."
                     /codon_start=1
                     /transl_table=11
                     /product="hypothetical protein"
                     /protein_id="HOQ53118.1"
                     /translation="MSSGTDEQPPVTGHPGIDEALGSLDLTGPVAEHAAGIQQAHAVL
                     QQVLNPPVQYPRQ"
     gene            5054..5803
                     /locus_tag="PLF56_05840"
     CDS             5054..5803
                     /locus_tag="PLF56_05840"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_015070779.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_function: GO:0008168 - methyltransferase activity
                     [Evidence IEA];
                     GO_process: GO:0032259 - methylation [Evidence IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="TlyA family RNA methyltransferase"
                     /protein_id="HOQ53119.1"
                     /translation="MTARLDAVLVRRGLARSRGQAAEAVRAGLVSVNGRPARKPSEPV
                     GDHDEVRLAEGDHYVSRAAHKLLGALDESGLAVSGRALDAGASTGGFTQVLLERGADL
                     VYAVDVGHDQLVPSVRTDPRVRVHEGLNLRDLTLGHLDGRPVDLVVGDVSFISLRLLV
                     RPLLAVLAPRGAALLLVKPQFEVGRGGLDSRGVVREAGARERAVDDVVRAAAEVGWHP
                     TWRGESRTPGASGNVEFFVLLRRPPTVGEPG"
     gene            5814..>6044
                     /locus_tag="PLF56_05845"
     CDS             5814..>6044
                     /locus_tag="PLF56_05845"
                     /inference="COORDINATES: protein motif:HMM:NF012235.2"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_function: GO:0005524 - ATP binding [Evidence IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="ATP-binding cassette domain-containing protein"
                     /protein_id="HOQ53120.1"
                     /translation="MPNNRYVTVTLHGGRPQAVSAAAGFVSGMTAHGITCLVKAEHLL
                     ERRIGALSGGELQRVLLALALLQQPDLLVLDEP"
BASE COUNT          836 a         2074 c         2214 g          920 t
ORIGIN      
        1 cggttcgtga cgcagcgtcc gcacgggatc agcgcggcca gggtcgctcc taccgtgggg
       61 gcgatagcgg caatcggccc tatcagggtg gtgaggggcc gggaaatcgt ggcactcgag
      121 gcggacaggg ccgtccgtac cggtccgatg gtgagggcga gcgtcgtgga gcaggccgtt
      181 ccgccgcggg tgacgaatcg ggtaaccggc gacagtctgg ccctcgggac gcaatgccga
      241 ggcgcggcgg ccaaagcggc caggatcgac ggccgtacca gcagggtcag cctcgcactt
      301 ccgccggcgc gcgtcgcacc gacggcgatc aggatcggcg gccgtatcag cagggtcagg
      361 ctcgtacgtc cgccggcgcg cgtcgtgccg acggagacca ggatcgacgg tcgtaccagc
      421 aggggcagcc gcgctcgtcc acccaggggt accgtgccga cgcgggtcag gatcgtcgcg
      481 gtccgcgagg cgtcgcccgg ggcgccgggg tcgccggcgg tgccgcagca gtcggtgcag
      541 cctggcgtgg gccggacgat cgccctcgcg ggggaagcgg acgcgaccgg gacgaccgcg
      601 agccccgtga gattccgccc ggcttggccc ccaaggccaa cgagccggca acgcccgtcg
      661 acgtcgacct cagcctgttg tcgcgtgggg tcaaggcgga gctgcgcagc ctcactgtcg
      721 agcacgccga ggtggtcgcg gctcacctcg ccgcggccgc ggagttgatc gacaccgacc
      781 ccgagttggc ctacgcgcac gcggaagcgg cccggcgccg ggcagctcgg ctacctgtgg
      841 tgcgtgaggc ggccggcacc actgcgtatg cggcgggtga gttcgcagca gcgctcaacg
      901 agttccgtgc cctgcgccgg atgaccggaa ccgacgactt tctggccgtg atggccgact
      961 gtgagcgggc tctgggcagg cccgcggcgg cactcaagct ggtcaaggag gggttggcgg
     1021 cccagccgcc gattgccagc cgggtcgagc tcaagctggt cgaagccggg gcccgtgacg
     1081 ccttgggtca acgggacgag gccgtgcgtc tgctgcgcca cgagatcgaa ctgctgggag
     1141 cgcgcgggcc caagaacgcg cgtgccaggc tgcgctactt ctttgcgcaa ctgctcgccg
     1201 aggcgggcag tgtggacgag gccgagaagt ggttcgcctc agcggtcagg ctggatcctg
     1261 acgacgagac cggtgcggcc gaggcgctgg ccgaactaca gggcatgagc atcgagttcg
     1321 acgacaccgc ggacgatctg tacgacgagc cggcgcaggc ggcgccggtg tcgacagcac
     1381 cggggtcgag cgcccagcaa ctggatcgag gcgagtattc aggcgcccag gcagcgcccg
     1441 ccacggcagc ggaccagtca gctgtttccg aaggcgagca cgaggactct tcgcaatcat
     1501 cggtcgaccc gtccttcgag caaccgtcga tcggcacctt cgacgagccg gaggaacggc
     1561 aggagttgcc ggtcgatttc agtgtcgaac aaccgtcgaa ccccacccaa ggggagccgg
     1621 aggaacggtc ggaagcgccg gtcgattcca gtgtcgaaca accgccgaac cccacctccg
     1681 gggagccgga ggaccggtcg gaggcgccgg tcgatccgtc ccttgagcaa ccgtcgaacc
     1741 ccacctccgg tgaggcagag gagaacgacc gatgagcgca cgcatcatcg acgatcacga
     1801 cgtcgccttc ttcgatctgg acggtgtgat ctacctgggg ccggtcgcgg tacccggcgc
     1861 cgtcgagggc atcacccgca tccgcgagac gggcgcgcgg gtgatgtacg tcaccaacaa
     1921 tgcggcgcgc agcgcggacg cggtggcgca ccacctcagc gcgttggggt tcgaggccgg
     1981 cccggacgat ctgatcacca gcgcgcaggt ggccgtcgac gtactggccg aacggcttcc
     2041 ggcgggcagc ctgctgctgg tggccggcac ccgcaacctg atcgagttgg cgcaggccgc
     2101 cgggtttcgc accacggcca gcgcggacga ccgtccggac gcggtgttgc agggctacga
     2161 ccccctgatc gagtggccgg tactggacga ggcggccatc gccgttcagc gcggtgccac
     2221 ctggtacggc accaacgacg acctcaaccg gcccaccaac cgggggctgg tgcccggcct
     2281 cggcgcgcag ttggccgtgg tgggcagcac ggtgcgacag cagccggtgg tattcggcaa
     2341 accgcacgcg ccgatgctcg actacgccgt cacgcacacc gcggcgcagc gtccgatctt
     2401 cgtcggcgac cgcatcgaca ccgacatcat gggcgcgaac cgcgccggta tgccgtccct
     2461 gttggtgttc accggcgcgc acggcaagcg tgacctgctc gaggccggcc ccggcgatcg
     2521 tccgagctac atcggcgctg acctgcgcgc actgctcgaa ccgccgcgga cggcagctca
     2581 gcacgggcac gcctggcact gcggcgacgc ggtggccgag ctgctcgagg gtgaagcgtc
     2641 ggtgcggggc gagctgtcga ctcaggcggg gcagttcgat gccctgtggg ccctggcgaa
     2701 cctggtctgg gccactgacg ccgacgcgct ggcggcgctg gcgttgctcg accaggtgcc
     2761 gtgacgcctt cgccgcctca ccacgccacc gcacgccgcc gctgctgagc atctggtcgt
     2821 gcgccggcaa gcaggggcgg cggcggtatt gtctggctga acgcgccacc ggggtgcgac
     2881 gcacgatcca ggagttaccg tgcccaccgg taccagatcc tctaccgccg accttgccta
     2941 catcgcggtg ttcgctgccc tgatcgccgt cttctcgctc gtgccggggg tgccgatcgg
     3001 tcccgtaccg atcaccctgc agaccctggg cgtcgccctg gccggcctgg tgctcggccc
     3061 ctggcgcggg ttcgccgcca ccgctctcta cctgctggtg ggtttcgccg gcctgcccgt
     3121 gttcgccggc ggagcgtccg gtctgggggt gctgggcaag cccagcatcg gctacctgct
     3181 cagcttcccg ttcgcggcgc tggtggccgg gttgctggct cgctggttgg gtggacgcaa
     3241 cccccggctg cggttcctgt ggctctttct ggccggcctc ggcgcgtcct ttctgatcgt
     3301 tcaccccgcg ggcatcgccg gcctgatgct cattctgcac atcgacttcg ccaaggccct
     3361 cgccatcgac ctggcgttct ggccgggcga cgtggccaag aacctggtgg cggccgccat
     3421 cgcgctggcc gtgcacaagg cgttccccgc cctgttggcg cagcgcgtgc cggtggccgc
     3481 ctgacgtgat ccaaccctga cgtgattcga ctgagcgcgg tctcggttcg ggtaccgagc
     3541 ctcgccccac cgggtttcaa gctgctgctg gacgaggtgc acctcgacct ggccgagcac
     3601 cgcatcgcgc tgatcggggc caacgggtcg ggcaagtcca ccctgctgcg cctactcaac
     3661 gggctgttgc tgccgaccca gggccgcgtg ctggtggatg gcctcgacac ccgcagcgac
     3721 gccgccgcgg tgctccggcg ggtcgggttc acgttcaccg atccgctcag ccaactggtg
     3781 ctgcccacgc cgctggacga cgtgcagctc agcctgcggg cccgcgtccg caaccggacg
     3841 gagcggcagg cggccgcgcg cgactggctg aaccgttacg ggctggccga ggtggccgag
     3901 cacagcatct acgacctctc ggggggtgag cgccaactgg cggcgctggt cagcgtgctc
     3961 gcggtcgagc cgtcggtgct ggtctgcgac gaaccgacca cgctgctcga cctgcgcaac
     4021 cgcaacctgg tcgccggggt gctggacgct ctgccgcacc aggtgattct tgccactcac
     4081 gatcttgaac tggccgcaca ggccgagcgg gtggtgctga tcgccggcgg acgcgtggcc
     4141 gccgacggca gcccgttcga ggtgatcgac acgtatcgac ggtcgatggc atgagaagca
     4201 ccgacgccct gttcggcaac tacctgcccg gcacgtcggt gtggcatcgc gcgggcgtcg
     4261 gctggaagta cctggtggtg ctcgtgctga cgctgccggc gctgatcgtg cagcagccgt
     4321 gggcgacaac tgtggcgctg gtcatcacgg tgctgggcct gctcagcacc ggtctgcccg
     4381 cgcgcgcaac gctggcgctg ccgtggggac tgatcgggct gctggcagtg ctggccctct
     4441 ttcaggttct ggtcggtcaa cccacccttg cgctggtggt gcccggcaac gtgctgctcg
     4501 ccgtgctggc ctcgcggctg ctgatcacca ccacccgggc agcggtgctg atcgacgcgc
     4561 tcgtggcggc cgccgaccgc atcccgttcg tggacggtga gcggttcgga ctcagcatcg
     4621 gcatcatgct gcgcagcgtg ccgtttctga ccggcgcctt ctacgacgtg cgggacgctg
     4681 cccgcgcccg tgggctggag cggcactgga tcgcccggct gacgccggtg gtggtgcgtg
     4741 ccgtcggttt cgcccagacg accggggacg cgctggccgc ccgcggcctg ggcgagcgtt
     4801 gatcacgcgg atgaccaggc gaccgattcg tcgggcgggt ctcgacgggc tcgcccagcg
     4861 tcgtccccgg taagctgacc ggcgtgagca gcggcacgga cgaacaaccg ccggtgaccg
     4921 gtcatcccgg catcgatgag gcgctgggct cgctcgacct gaccgggccg gtcgccgagc
     4981 acgcggcggg cattcaacag gcacacgccg tgctgcagca ggtgctcaac ccgccggtgc
     5041 agtatccgcg acagtgaccg ctcgcctcga cgccgtcctg gtcaggcgtg ggctggcacg
     5101 ctccaggggt caggcggccg aggcggtccg ggccggcctg gtcagcgtca acggacgtcc
     5161 ggcgcgcaag ccgtccgaac cggtcggcga ccacgacgag gtgcggctgg cagagggcga
     5221 ccactacgtc tcgcgagcgg cgcacaagct gctcggcgcg ctggacgagt cggggctggc
     5281 ggtgagcggc cgggcgctgg acgcgggcgc gtccaccggt ggcttcaccc aggtgctgct
     5341 cgaacgcggc gccgacctgg tgtacgcggt cgacgtcggg cacgaccagc tggtgccgag
     5401 cgtgcggacc gacccgcgcg tgcgcgtcca cgagggcctc aacctgcgcg acctcaccct
     5461 gggtcacctg gacggcaggc cggtcgacct ggtcgtcggc gacgtgtcgt tcatctcact
     5521 gcggctgctg gtgcggccgc tgctggcggt gctggcaccg cggggcgcgg cgctgctgtt
     5581 ggtgaagccc cagttcgagg tgggacgcgg ggggctcgac tcccgtggtg tggtgcgcga
     5641 agccggggca cgcgagcgag cggtcgacga tgtggtgcga gccgccgccg aggtggggtg
     5701 gcacccgacc tggcgtggtg agagtcgcac gcccggtgcg tccggcaatg tggagttctt
     5761 cgtgctgctg cgacgcccgc caactgtcgg cgagccgggt tagggtgagt gccgtgccca
     5821 acaatcgcta tgtcaccgtc accctgcacg gcggccgtcc acaggcggtg agtgcggcgg
     5881 cgggtttcgt cagcggcatg acggcccacg gcatcacctg cctggtcaag gccgagcatc
     5941 tcttggagcg gaggatcggt gccctctccg gcggcgagtt gcagcgggtc ctgctggccc
     6001 tggccctgct ccaacagcct gatttgctgg tgctggatga accc
//
LOCUS       DAOSUG010000135         5999 bp    DNA     linear   ENV 10-JUN-2024
DEFINITION  MAG TPA_asm: Micropruina sp. isolate AMR_MDS_2195
            NODE_6144_length_5999_cov_4.412517, whole genome shotgun sequence.
ACCESSION   DAOSUG010000135 DAOSUG010000000
VERSION     DAOSUG010000135.1
DBLINK      BioProject: PRJNA925477
            BioSample: SAMN32864262
            Sequence Read Archive: SRR4236673
KEYWORDS    WGS; ENV; Metagenome Assembled Genome; MAG; Third Party Data; TPA;
            TPA:assembly.
SOURCE      Micropruina sp. (wastewater metagenome)
  ORGANISM  Micropruina sp.
            Bacteria; Bacillati; Actinomycetota; Actinomycetes;
            Propionibacteriales; Nocardioidaceae; Micropruina.
REFERENCE   1  (bases 1 to 5999)
  AUTHORS   Abdulkadir,N., Saraiva,J.P., Zhang,J., Stolte,S., Gillor,O.,
            Harms,H. and Rocha,U.
  TITLE     Genome-centric analyses of 165 metagenomes show that mobile genetic
            elements are crucial for the transmission of antimicrobial
            resistance genes to pathogens in activated sludge and wastewater
  JOURNAL   Microbiol Spectr 12 (3), e0291823 (2024)
   PUBMED   38289113
REFERENCE   2  (bases 1 to 5999)
  AUTHORS   Abdulkadir,N.
  TITLE     Direct Submission
  JOURNAL   Submitted (24-MAR-2023) Environmental Microbiology, Helmholtz
            Center for Environmental Research -UFZ, Permoserstr 15, Leipzig
            04318, Germany
COMMENT     The annotation was added by the NCBI Prokaryotic Genome Annotation
            Pipeline (PGAP). Information about PGAP can be found here:
            https://www.ncbi.nlm.nih.gov/genome/annotation_prok/
            
            ##Genome-Assembly-Data-START##
            Assembly Method        :: SPAdes v. 3.11.1
            Genome Representation  :: Full
            Expected Final Version :: Yes
            Genome Coverage        :: Not Applicable
            Sequencing Technology  :: Illumina MiSeq
            ##Genome-Assembly-Data-END##
            
            ##Genome-Annotation-Data-START##
            Annotation Provider               :: NCBI
            Annotation Date                   :: 05/04/2023 04:57:10
            Annotation Pipeline               :: NCBI Prokaryotic Genome
                                                 Annotation Pipeline (PGAP)
            Annotation Method                 :: Best-placed reference protein
                                                 set; GeneMarkS-2+
            Annotation Software revision      :: 6.5
            Features Annotated                :: Gene; CDS; rRNA; tRNA; ncRNA
            Genes (total)                     :: 2,776
            CDSs (total)                      :: 2,750
            Genes (coding)                    :: 2,714
            CDSs (with protein)               :: 2,714
            Genes (RNA)                       :: 26
            tRNAs                             :: 24
            ncRNAs                            :: 2
            Pseudo Genes (total)              :: 36
            CDSs (without protein)            :: 36
            Pseudo Genes (ambiguous residues) :: 0 of 36
            Pseudo Genes (frameshifted)       :: 2 of 36
            Pseudo Genes (incomplete)         :: 32 of 36
            Pseudo Genes (internal stop)      :: 2 of 36
            ##Genome-Annotation-Data-END##
FEATURES             Location/Qualifiers
     source          1..5999
                     /organism="Micropruina sp."
                     /mol_type="genomic DNA"
                     /submitter_seqid="NODE_6144_length_5999_cov_4.412517"
                     /isolate="AMR_MDS_2195"
                     /isolation_source="Activated sludge of municipal
                     wastewater treatment plants"
                     /db_xref="taxon:2737536"
                     /environmental_sample
                     /geo_loc_name="Canada"
                     /lat_lon="49.25 N 123.1 W"
                     /collection_date="2015-06-16"
                     /metagenome_source="wastewater metagenome"
                     /note="metagenomic"
     gene            complement(172..1065)
                     /locus_tag="PLF56_05850"
     CDS             complement(172..1065)
                     /locus_tag="PLF56_05850"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_013161512.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="VOC family protein"
                     /protein_id="HOQ53121.1"
                     /translation="MQKIVPSIWFDHTAAEAVAFYTEHFPDSRVLHEQRYPSTGLPDF
                     QREFAGEVLAIDFELRGFRFSAINAGPEFEVNQSISFTLPFDEAADPDARTTMQTLWT
                     ALAEGGQVMMPLAEYPFNPYYGWVRDRFGVGWQFILSDPQAGPGPAVLTSLLFGAQAQ
                     NRADEALAFYSSLFPDGRITMVARYPQPSGPAPEGAVMYSTMMVFDQSFTLMDSAVEQ
                     PDTFSPGVSLMLLCADQADIDHYWTALSAVPEAEQCGWCVDRFGVSWQVVPADLNEYL
                     LTPESYGAMLQMKKIELSGLG"
     gene            complement(1148..1870)
                     /locus_tag="PLF56_05855"
     CDS             complement(1148..1870)
                     /locus_tag="PLF56_05855"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_011755401.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="HEAT repeat domain-containing protein"
                     /protein_id="HOQ53122.1"
                     /translation="MGTKRSAYADVLRALPPAEIPAYLDAHSHLPGPRGNLELIDAAS
                     DVLPRELALELAAAAGEFLACCGVATLGRLALDDPDDPRPDELLTHYAGDARWRVREA
                     AAMAGQRIGDADPVRLRRLVERWTASSDPLVVRCGIATVCEPRLLRDPRTATAALAAC
                     GRATQQLVGLPPERRRQADVRTLRQALGYCWSVAIAADPAPGLRAFGALDTTDPDVAW
                     VICENRRKRRLAVLLAAGEPAS"
     gene            complement(1989..3227)
                     /locus_tag="PLF56_05860"
     CDS             complement(1989..3227)
                     /locus_tag="PLF56_05860"
                     /inference="COORDINATES: protein motif:HMM:NF021134.2"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_function: GO:0016758 - hexosyltransferase activity
                     [Evidence IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="glycosyltransferase 87 family protein"
                     /protein_id="HOQ53123.1"
                     /translation="MTGRWGRGSTLAAVAVLGVAVRWRFLDFQSGDYRTFLSRWYAFI
                     STNGHFAALHDDSFSNYNTPYLVLLALTSYLPIPPIVAIKTLSILGDLALAGVAAAIV
                     LRLRPGARWAPVAAFGVVLLLPTVVMNSGVWGQCDSLYAAASLACVLCLLEGRSGAAS
                     AWFGLAFGFKLQAVFLLPALVAVVIINRHRWVSLLAAPAAFVATLVPALLAGRSLVSQ
                     LMVYPLQITDPSGTGGTVGGPGAGRRPPGGGGSGFVLNDGQSFTHNAPTPYAWLPADA
                     SVLWKYAGLALTVGVVTGLGVWLLARGQALSPTQIVLLAATSTLIIPLLLPEMHERYF
                     YLAEVLLVVAGFADGWFWWPAAGMQAASITTYLSYLLNHTTVPLGWAAVVATASGLIA
                     AVLLLRTLRAHPPGSPLFVG"
     gene            complement(3315..4715)
                     /locus_tag="PLF56_05865"
     CDS             complement(3315..4715)
                     /locus_tag="PLF56_05865"
                     /inference="COORDINATES: protein motif:HMM:NF024808.2"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_component: GO:0055052 - ATP-binding cassette (ABC)
                     transporter complex, substrate-binding subunit-containing
                     [Evidence IEA];
                     GO_function: GO:0042626 - ATPase-coupled transmembrane
                     transporter activity [Evidence IEA];
                     GO_function: GO:0140359 - ABC-type transporter activity
                     [Evidence IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="sugar ABC transporter substrate-binding protein"
                     /protein_id="HOQ53124.1"
                     /translation="MFRFTRKAGAALATVAALTLTMTACSGEAAPTTTAGATTGGDAI
                     DTSTASGEVTYWLWDNNQKPAYQACADAFHTANPNVTVKISQYAWDDYWSGLTNGFVA
                     GTAPDVFTNHLSKYPEFVTQGQLQPLGATLEKDGIKTDIYQKGLADLWVGQDGQRYGL
                     PKDFDTVAIFYNKKLIKDAGVQEADLQNLTWNPTDGGTYEKTIAHLTVDANGKRGDEA
                     GFDKTKVKVYGLGLDGGSGGGNGQTQWSMYSGTTGWQFTDKNPWGTHYNYDKPEFQNT
                     MTWMRSLIEKGYMPSVEAVTGQSSADIYGAGKYAMITNGSWMINQMFGYKGVETGLAP
                     TPVGASGKRSSMYNGLADSVWSGSKNKPAAIKWVEYLGSSACQDIVGKAGAVFPAIPS
                     GTELAEAAFKAKGIDVSPFTVQVKDGTTFLFPITDHASQITNIMQPAVDAVFTGKAKA
                     DTLTAANDQVNQLFVG"
     gene            complement(4810..5739)
                     /locus_tag="PLF56_05870"
     CDS             complement(4810..5739)
                     /locus_tag="PLF56_05870"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_016666044.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_process: GO:0008643 - carbohydrate transport [Evidence
                     IEA];
                     GO_process: GO:0055085 - transmembrane transport [Evidence
                     IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="carbohydrate ABC transporter permease"
                     /protein_id="HOQ53125.1"
                     /translation="MSTVAEVALADAEAKQRRRRMSPGRIGAWAVMVVFLILTIFPFL
                     WMIRTAFSTTRSLPSDPNSLLPVDFTWGAFQRVLGLGTAEQAIAEGGSGASVNFWLYL
                     RNSFFYAGAVTIGQILFCSMAAYAFARLRWPGRNLVFGVFLGALLVPPIFTSLPNFIL
                     IKNLGLLNTLPGMILPTFLMTPFAIFFLRQFFLGINREIEEAAAIDGAGPWRIFFRII
                     LPMSTAPITTLAILTFIATWNDYFWPLLVGNTEQTKVLTVALGVFRSQTPAGSPDWAG
                     LMAATLLTALPVLILFFAFARKIIDSIGYSGSK"
     gene            complement(5752..>5999)
                     /locus_tag="PLF56_05875"
     CDS             complement(5752..>5999)
                     /locus_tag="PLF56_05875"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_020387060.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=3
                     /transl_table=11
                     /product="sugar ABC transporter permease"
                     /protein_id="HOQ53126.1"
                     /translation="LAMVLVITVTGSFQVFDTVAVTTRGGPVNVTRVFQYYIYQKAFG
                     EGQFGYGSALSVILFIILGALAVLQLRLLRSGESDLA"
BASE COUNT         1030 a         1956 c         2109 g          904 t
ORIGIN      
        1 ccgaaagcgc gagtcagccg agaccgctca gccgtggacg ctcagccgag gacgcgaagc
       61 cgaggacgct cagctgaggc cgctcagccg aggtctcgct cagctgaggc cgctcagccg
      121 tggacgctca gccgaggcag cccagccgag gcagcccagc cgaggcctcg ctcagccgag
      181 gccgctcagc tcgatcttct tcatctgcag catcgccccg tagctttcgg gggtgagcag
      241 gtactcgttc aggtcggccg gcaccacctg ccagctgacc ccgaaccgat ccacgcacca
      301 gccgcactgc tcggcctcgg gcaccgcgct gagcgccgtc cagtagtggt cgatgtcggc
      361 ctggtcggcg cacaacagca tcagtgacac acccggcgag aaggtgtcgg gctgctcgac
      421 ggccgaatcc atcagggtga acgactggtc gaacaccatc atcgtcgagt acatcaccgc
      481 gccctcgggg gccgggccgg acggctgggg gtagcgcgcg accatcgtga tgcgtccgtc
      541 ggggaacaag gagctgtaga aggcgagcgc ctcgtcggcc cggttctgtg cctgggcccc
      601 gaacagcagc gaggtgagca ccgccggccc cggcccggcc tgcgggtcgg agagaatgaa
      661 ctgccagccc acgccgaagc ggtcacgcac ccacccgtag tacgggttga acgggtactc
      721 ggcgagcggc atcatcacct ggccaccctc ggccagggcg gtccagagcg tctgcatcgt
      781 ggtgcgtgcg tcggggtcgg cggcctcgtc gaagggcagg gtgaacgaga tggactggtt
      841 gacctcgaac tcggggccgg cgttgatcgc agagaaccgg aagccacgca actcgaagtc
      901 gatggccagt acctcaccgg cgaactcgcg ctgaaagtca ggcaggccgg tgctcgggta
      961 gcgctgctcg tgcagcaccc gcgagtcggg gaagtgctcg gtatagaacg ccaccgcctc
     1021 tgcggccgtg tggtcgaacc agatgctggg cacgatcttc tgcatggcag aacctcctcg
     1081 tcgggtgccg catacggtag cggcccctgc cgacggtttc atcgctcggg cgtcgaagcc
     1141 ctgccgatca actcgcgggt tcaccggcgg ccagcagtac ggccagtcgg cgcttgcggc
     1201 ggttctcgca gattacccag gccacgtccg gatctgtggt gtcgagggcg ccgaaggcgc
     1261 gaagccctgg cgcggggtcg gccgcgatgg cgaccgacca gcagtagccg agtgcctgcc
     1321 gcagcgtacg gacgtccgcc tgccggcgcc gctcgggcgg caatccgacc agctgctgcg
     1381 tcgcacgccc gcatgccgcc agtgccgcgg tagccgtccg ggggtcacgg agcaggcggg
     1441 gttcgcacac cgtggcgatc ccgcagcgca ccaccagggg gtcggacgac gccgtccagc
     1501 gttcgaccag cctgcgcagc cggacggggt ccgcgtcgcc gatccgctga ccggccatcg
     1561 ccgccgcctc acgcacgcgc caccgcgcgt caccggcgta gtgggtgagc agctcatcgg
     1621 gtcggggatc gtccgggtcg tcgagggcga gccggcccaa cgtggccacc ccgcaacaag
     1681 ccaggaactc acccgcagcg gcggccagtt cgagcgccag ttcgcggggc agcacatcgg
     1741 acgcggcgtc gatcagttcg aggttgccgc gcgggcccgg caggtggctg tgcgcgtcca
     1801 ggtaggcagg gatctcggcg ggcggcagcg cccgcagcac atccgcgtac gccgagcgtt
     1861 tcgtgcccat gaacccacgg tatcggcgtg cggaccgggg ggacgaccct ccgcccagcg
     1921 cccccctaac catctctggc ctgcgtagct acgcaggcca gagatagtta ggcgggcgtt
     1981 gggccgggtc agccgacgaa cagcggactt ccaggtgggt gcgcgcgcag tgttcgcagc
     2041 agcagcaccg cggcgatcag tcccgaggcc gtcgccacca ccgcggccca acccagaggc
     2101 acggtggtgt ggttgagcag ataactgaga taggtggtga tactggccgc ctgcatgccg
     2161 gcggccggcc accagaacca tccatccgcg aagccggcga ccaccagcaa cacctcggcc
     2221 aggtagaagt accgctcgtg catctcgggc agcagcagcg ggatgatcag ggtagacgtg
     2281 gcggccagca gcacgatctg cgtcggtgag agcgcctgcc cccgggccag caaccacacc
     2341 ccgaggccgg tcaccacgcc caccgtcagg gcgagtcccg cgtacttcca cagcacggac
     2401 gcatccgcgg gcagccaggc gtacggcgtc ggtgcgttgt gggtgaagct ctggccgtca
     2461 ttcagcacga agccgctccc accgccgccc ggtggccggc gcccggcacc cggtccgccc
     2521 accgtgccac cggtgccgga gggatcggtg atctggagcg ggtacaccat gagttggctg
     2581 accaggctgc gccccgccag tagggcgggc accagcgtgg cgacgaaggc ggccggggcg
     2641 gccagcagac tcacccaccg gtggcggttg atgatcacca ccgcgacgag cgcgggcagc
     2701 aggaacaccg cctgcagctt gaagccgaac gcaagcccga accatgcgga cgcggcccca
     2761 gagcgtccct cgagcaggca cagcacgcag gccagtgacg cggcggcgta cagcgagtcg
     2821 cactggcccc acactccact gttcatcacg acggtcggca gcagcaatac cacgccgaag
     2881 gcagcaaccg gtgcccacct cgcacccgga cgaagccgca gcacgatcgc cgcggccacc
     2941 ccggcaagtg ccagatcgcc caggatcgac agggttttga tcgccacgat cggtgggatc
     3001 ggaaggtagc tggtgagggc gagcagcacc agatagggcg tgttgtagtt cgagaacgag
     3061 tcgtcgtgca gggcggcgaa atgtccgttc gtcgagatga aggcgtacca gcgcgacagg
     3121 aacgtccggt agtcgccgga ctggaaatcc aggaaccgcc agcgcacggc gacacccagc
     3181 acggccaccg ccgccagcgt cgacccgcgc ccccatcgtc cggtcacgtc tcctcctccc
     3241 tgcgccgagc gcccccaact atctctggcc cgcgtagcta tgcaggccag agatagttag
     3301 gggggcgtgg agcgtcagcc cacgaacagc tggttcacct ggtcgttggc cgcggtcaga
     3361 gtgtcggcct tggccttgcc ggtgaacacc gcgtccaccg ccggctgcat gatgttggtg
     3421 atctgcgagg cgtggtcggt gatcgggaac aggaaggtcg tgccgtcctt gacctgcacg
     3481 gtgaacggcg agacgtcgat gcccttggcc ttgaacgcgg cctcggcgag ctcggtaccc
     3541 gacgggatcg ccgggaagac cgcgccggcc ttaccgacga tgtcctggca ggcggacgat
     3601 ccgaggtact cgacccactt gatcgcggcc ggcttgttct tcgaacccga ccacaccgag
     3661 tcggccaggc cgttgtacat gctggagcgc ttgccggacg caccgaccgg ggtgggggcc
     3721 aggccggtct cgacaccctt gtagccgaac atctggttga tcatccacga gccgttggtg
     3781 atcatggcgt acttgcccgc gccgtagatg tcggccgagc tctggccggt gacggcctcg
     3841 acgctgggca tgtagccctt ctcgatcagc gagcgcatcc aggtcatggt gttctgaaac
     3901 tcgggcttgt cgtagttgta gtgggtgccc caggggttct tgtcggtgaa ctgccagcct
     3961 gtcgtgccgg agtacatgct ccactgggtc tggccgttgc caccacccga gccgccgtcc
     4021 aggccgaggc cgtacacctt gaccttggtc ttgtcgaagc cggcctcgtc gccgcgcttg
     4081 ccgttggcgt caacggtcag gtgggcgatg gtcttctcgt acgtgccgcc gtcggtcggg
     4141 ttccaggtca ggttctgcag atcggcctct tggacgccgg cgtccttgat cagcttcttg
     4201 ttgtagaaga tcgcgacggt gtcgaagtcc ttgggcaggc catagcgctg gccgtcctgg
     4261 ccgacccaga gatcggcgag gcccttctgg tagatgtcgg tcttgatgcc gtccttctcg
     4321 agggtggcgc cgagcggctg gagctggccc tgggtgacga actcggggta cttcgacagg
     4381 tggttggtga agacgtcggg ggcggtgccg gcgacgaagc cgttggtgag gcccgaccag
     4441 tagtcgtccc aggcgtactg gctgatcttg acggtgacgt tcgggttcgc ggtgtggaac
     4501 gcgtcggcgc aggcctggta ggcgggcttc tggttgttgt cccagagcca gtaggtgacc
     4561 tcgccggagg cggtgctggt gtcgatcgcg tcgccaccgg tggtcgctcc ggcggtcgtg
     4621 gtgggtgctg cctcgccgct gcaggcggtc atggtgaggg tcagggcggc gacggtcgcg
     4681 agggcggcgc cggccttgcg ggtgaagcgg aacatgggcg attcctttcg ggagtcagta
     4741 cgtgggcatg cgtggtcggg cggacgatgc tgcgtccgcg gtcggtggcg gagtggggga
     4801 cggagcgggc tacttcgagc cgctgtaacc gatcgagtcg atgatcttgc gggcgaaagc
     4861 gaagaacagg atgagcaccg gcagcgcggt gagcagggtg gcggccatca ggccggccca
     4921 gtccggcgac ccggccggag tctgcgaacg gaacacgccc agggccacgg tgagcacctt
     4981 ggtctgttcg gtgttgccga ccaacagtgg ccagaagtag tcgttccagg tggcgatgaa
     5041 ggtgaggatc gcgagcgtgg tgatcggcgc ggtgctcatc ggcagaatga tccggaagaa
     5101 gatccgccat ggccctgcgc cgtcgatcgc ggccgcctct tcgatctcgc ggttgatgcc
     5161 gagaaagaac tggcgcagaa agaagatcgc gaacggcgtc atcaggaagg taggcagaat
     5221 catgcccggc agcgtgttca gcagcccgag gttcttgatc aggatgaagt tcggcaggct
     5281 ggtgaagatc ggcggcacca gcagtgcgcc gagaaacacg ccgaagacca ggttgcgtcc
     5341 gggccagcgc agccgggcga aggcataggc cgccatcgag cagaacagga tctgaccgat
     5401 ggtgaccgcc cccgcgtaga agaacgagtt gcgcaggtag agccagaagt tcaccgacgc
     5461 tccggaaccg ccctcggcga tcgcctgctc ggcggtgccg agccccagca cgcgctggaa
     5521 ggcaccccag gtgaagtcga ccggcaacag cgagttcggg tcggacggca gcgagcgggt
     5581 ggtcgagaac gccgtgcgga tcatccacag gaacggaaaa atggtgagga tcaggaacac
     5641 caccatcacg gcccaggcac cgatccggcc ggggctcatc cggcgccggc gctgcttggc
     5701 ctcggcgtcg gcgagagcga cttctgcaac ggttgacatg gccatactcc tttacgccag
     5761 atccgactcg cccgagcgca gcagccgcag ctgcagcacc gccagggcac cgagaatgat
     5821 gaagaggatc accgacaggg cgctgccgta gccgaactga ccctcgccga acgccttttg
     5881 gtagatgtag tactggaaca cccgggtgac gttgacggga ccgccccggg tggtcaccgc
     5941 caccgtgtcg aacacctgga acgagccggt cacggtgatc accagcacca tcgccagca
//
LOCUS       DAOSUG010000136         5999 bp    DNA     linear   ENV 10-JUN-2024
DEFINITION  MAG TPA_asm: Micropruina sp. isolate AMR_MDS_2195
            NODE_6145_length_5999_cov_3.514132, whole genome shotgun sequence.
ACCESSION   DAOSUG010000136 DAOSUG010000000
VERSION     DAOSUG010000136.1
DBLINK      BioProject: PRJNA925477
            BioSample: SAMN32864262
            Sequence Read Archive: SRR4236673
KEYWORDS    WGS; ENV; Metagenome Assembled Genome; MAG; Third Party Data; TPA;
            TPA:assembly.
SOURCE      Micropruina sp. (wastewater metagenome)
  ORGANISM  Micropruina sp.
            Bacteria; Bacillati; Actinomycetota; Actinomycetes;
            Propionibacteriales; Nocardioidaceae; Micropruina.
REFERENCE   1  (bases 1 to 5999)
  AUTHORS   Abdulkadir,N., Saraiva,J.P., Zhang,J., Stolte,S., Gillor,O.,
            Harms,H. and Rocha,U.
  TITLE     Genome-centric analyses of 165 metagenomes show that mobile genetic
            elements are crucial for the transmission of antimicrobial
            resistance genes to pathogens in activated sludge and wastewater
  JOURNAL   Microbiol Spectr 12 (3), e0291823 (2024)
   PUBMED   38289113
REFERENCE   2  (bases 1 to 5999)
  AUTHORS   Abdulkadir,N.
  TITLE     Direct Submission
  JOURNAL   Submitted (24-MAR-2023) Environmental Microbiology, Helmholtz
            Center for Environmental Research -UFZ, Permoserstr 15, Leipzig
            04318, Germany
COMMENT     The annotation was added by the NCBI Prokaryotic Genome Annotation
            Pipeline (PGAP). Information about PGAP can be found here:
            https://www.ncbi.nlm.nih.gov/genome/annotation_prok/
            
            ##Genome-Assembly-Data-START##
            Assembly Method        :: SPAdes v. 3.11.1
            Genome Representation  :: Full
            Expected Final Version :: Yes
            Genome Coverage        :: Not Applicable
            Sequencing Technology  :: Illumina MiSeq
            ##Genome-Assembly-Data-END##
            
            ##Genome-Annotation-Data-START##
            Annotation Provider               :: NCBI
            Annotation Date                   :: 05/04/2023 04:57:10
            Annotation Pipeline               :: NCBI Prokaryotic Genome
                                                 Annotation Pipeline (PGAP)
            Annotation Method                 :: Best-placed reference protein
                                                 set; GeneMarkS-2+
            Annotation Software revision      :: 6.5
            Features Annotated                :: Gene; CDS; rRNA; tRNA; ncRNA
            Genes (total)                     :: 2,776
            CDSs (total)                      :: 2,750
            Genes (coding)                    :: 2,714
            CDSs (with protein)               :: 2,714
            Genes (RNA)                       :: 26
            tRNAs                             :: 24
            ncRNAs                            :: 2
            Pseudo Genes (total)              :: 36
            CDSs (without protein)            :: 36
            Pseudo Genes (ambiguous residues) :: 0 of 36
            Pseudo Genes (frameshifted)       :: 2 of 36
            Pseudo Genes (incomplete)         :: 32 of 36
            Pseudo Genes (internal stop)      :: 2 of 36
            ##Genome-Annotation-Data-END##
FEATURES             Location/Qualifiers
     source          1..5999
                     /organism="Micropruina sp."
                     /mol_type="genomic DNA"
                     /submitter_seqid="NODE_6145_length_5999_cov_3.514132"
                     /isolate="AMR_MDS_2195"
                     /isolation_source="Activated sludge of municipal
                     wastewater treatment plants"
                     /db_xref="taxon:2737536"
                     /environmental_sample
                     /geo_loc_name="Canada"
                     /lat_lon="49.25 N 123.1 W"
                     /collection_date="2015-06-16"
                     /metagenome_source="wastewater metagenome"
                     /note="metagenomic"
     gene            complement(<1..691)
                     /locus_tag="PLF56_05880"
     CDS             complement(<1..691)
                     /locus_tag="PLF56_05880"
                     /inference="COORDINATES: protein motif:HMM:NF014808.2"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_function: GO:0070006 - metalloaminopeptidase activity
                     [Evidence IEA];
                     GO_process: GO:0006508 - proteolysis [Evidence IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="M17 family peptidase N-terminal
                     domain-containing protein"
                     /protein_id="HOQ53127.1"
                     /translation="MSRSPNKLIPDDFTPIPSLQLECDVRAVDQFSSELDAVALLVAA
                     GTDLAERTGLDADALAAAGFDAEPGSSLVIAQQDRPLVLVGLGSDEPTADSLRDAAAA
                     AARASAKKGGRLGIEVPPLGLDALTAGQVITEGARLARYRYTALQRKPKEQPLSVLEV
                     KIAGADADQASQGIDRAAIGVRAASITRDVANTPAGHLTATDFAAVADELGAKFGFSV
                     RAYDKTELIEMG"
     gene            1016..2575
                     /locus_tag="PLF56_05885"
     CDS             1016..2575
                     /locus_tag="PLF56_05885"
                     /inference="COORDINATES: protein motif:HMM:NF015557.2"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_component: GO:0016020 - membrane [Evidence IEA];
                     GO_process: GO:0055085 - transmembrane transport [Evidence
                     IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="SLC13 family permease"
                     /protein_id="HOQ53128.1"
                     /translation="MSPVTIVLVILVGAVVAFLSNRVPLGVVALGVAVTLWASGVLTL
                     QQALAGFGDPTVIFIAGLFVVSDSLDATGVTAWAGRQVINHGGGSRVRLLLLIGGLVA
                     VLTALISVNGSVAALGPVVVVVAIRAGIAPSQLLIPLAFAAHAGSLLLLTGTPVNILV
                     SDAAADAGARPFGFFEFALAGLPLLAGTLLIIVLFGQRLLPHRPPSAMSPDVSEHVRI
                     LQAQYELPTDTQLVGARRGVTEVVIPPRSELIGLRVFPGMSTPSGDLVVLGLQRAGEP
                     LGGSDTTLETGDVMLLGGTWQHLEHHTSGSEVLVVDPPDALRRSIPLGAGAKRSIAVL
                     AAMVLLLATGLVPPAIAAVLAALALVLTGVRTSAEAYRAVNWTTVVLVGGMIPLSTAF
                     ISTGAADLIAEGVLTMVGGSSPYLALLVMCVLTMILGQLISNMATVLIMLPIATVLAA
                     DLHVSVLPFMMALTVAGAAAFLTPVATPANTMVFEPGGYVFSDYWKLGLPILLLFLAV
                     AVFWVPFVWPF"
     gene            2908..5493
                     /locus_tag="PLF56_05890"
     CDS             2908..5493
                     /locus_tag="PLF56_05890"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_011755556.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_function: GO:0005507 - copper ion binding [Evidence
                     IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="multicopper oxidase domain-containing protein"
                     /protein_id="HOQ53129.1"
                     /translation="MRRGRSPWRDWPLIGWLSIAVAVALGHRWLAESQWLLLHLVFLG
                     AVTHSIMVWSMHFAQTLLRQPPPAHQDLRLALLTLGSALVFIGVPTANWWLTLVGGSI
                     VAVTVGWHAWLLLRMVRTALPARFRVTVRYYIAAGCSLALGAGFGIALAWGWTDSWHG
                     RLLVAHALTNVLGWLGLTVTGTLLTLWPTMLRTRMDASAEALTVRALPVLLTGIAVAV
                     GGALIGVTWIGALGLVAYLAGFALWAGALWRPLRAKPITEFAPASVAAALCWLVIGLT
                     WAGWVLVSAGDWVQVREQFIWPAAALAGGFGVQILTGALSYLLPSVLGGGPSVVRAGQ
                     RFFNAAAGFRLVAVNGGLVLWLAPTPSWVKVTGSLLALGAAASFLPILVLGLKASIAA
                     RRAVAKGEQPEPPTERPSPFAATQLIAGLTAVAVAVVVGVGVDPHAAGVAAAPAATQV
                     APTGRTVRVSVEIVAMHYVPSSVSVHPGDTLVVDLVNSDTNTHDLVIGEARTARLGPG
                     EKATLDAGVIGATVQGYCSIVGHKQMGMTFDVNVVGGPATSPTASAAPDGHAGHDSST
                     GTQPLSAVIDPVLAPLSAEKVHRLTLTAQELPLEVAPGVWQRRWTFNGRAPGPTLHGR
                     VGDVFEITLVNGGTIGHSIDFHAGSLAPDRPMRTIQPGESLVYRFTATKAGVWLYHCS
                     TMPMSTHIAAGMFGAVVIEPDGLPTVDRSYLLVQSETYLQTRAPDAVSATEVNADAIA
                     AEQPSYVAFNGVANGYDQVPLEATAGERVRFWVLDAGPNRPSSFHIVGAQFDTVYVEG
                     HYELRRGKDGFGGTDGGSQALGLQAAQGGFVEAVFHEAGRYPMVTHVMSDAERGAHGY
                     VVVRP"
     gene            5559..5861
                     /locus_tag="PLF56_05895"
     CDS             5559..5861
                     /locus_tag="PLF56_05895"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_016669301.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="DUF2249 domain-containing protein"
                     /protein_id="HOQ53130.1"
                     /translation="MNQSVPIETIEIAEASGHTCNCGEHGAAAPVLDVRQIPHAIRHG
                     AVFGALEQLGAGGVLALVAPHNPLPLLNQLAQRWPIDIAYLEDGPAAWQLRITRRA"
BASE COUNT          775 a         2057 c         2122 g         1045 t
ORIGIN      
        1 agcccatttc gatcagttcg gtcttgtcat aggcgcgcac cgagaagccg aacttcgccc
       61 cgagctcgtc ggccacggcc gcgaagtcgg tggcggtgag gtgcccggcc ggggtgttgg
      121 ccacgtcgcg ggtgatggac gcggcccgta ccccgatcgc tgcccggtcg atgccctggc
      181 tcgcctggtc ggcgtcggcc ccggcgatct tgacttccag cacgctcagc ggctgctcct
      241 tgggtttgcg ctgcagtgcg gtgtagcggt agcgggccag ccgagcaccc tcggtgatca
      301 cctggccggc agtgagggcg tccaggccga ggggtggcac ctcgatgccg agccgtccgc
      361 ccttcttggc gctcgcgcgg gccgcggcgg ccgcggcgtc ccgcagggag tcggcggtgg
      421 gctcgtccga acccagcccg acgagcacca gcggacggtc ctgctgggcg atcaccaggc
      481 tgctgcccgg ttccgcatcg aagccggcgg cggccagcgc gtccgcatcg aggccggtcc
      541 gctccgcgag atcggtgccg gctgcgacga gcagggcgac cgcgtccaac tcggaggaga
      601 actggtcgac ggcacgcacg tcgcattcga gttgcaggga aggaatcgga gtgaaatcgt
      661 ccggaatgag tttgttgggg cttcgcgaca tcacaatctc ctggtctacg acgttgcggc
      721 gagttcttcg ccgtcaatag caacatactg cggcaaccac ggccctggcc ggtttgccga
      781 tggaacgctc gaataggtga gcggcggggt ggcgcggcga gatgggccct cgtggggcgg
      841 ctcgcgggcg acgggctggg tgcgatcgtc ggggtccatg caccgcgcgg ccgacgcgat
      901 ggcagactgg ggcagctcct gggccgggga gctccgaggg tgcgtgctca gtagccgcgc
      961 gacagaggcc atgtcgggcg acacgtcagc attgccggcg tactagagtc ccgctgtgag
     1021 ccccgtgacg atcgtcctgg tcatactcgt gggcgccgtt gtcgccttct tgtcgaaccg
     1081 ggtgccgctg ggggtggtcg cactcggcgt ggcggtcacg ctgtgggcca gcggggtgtt
     1141 gaccctgcaa caggcattgg cgggcttcgg cgacccgacg gtgatcttca tcgcgggcct
     1201 gttcgtggtc tcggactcat tggacgccac cggtgtgacg gcctgggccg gccgacaggt
     1261 gatcaaccat gggggcggca gccgagtgcg gctgctactg ctgatcggtg gcctggtggc
     1321 cgtgctgacc gccctgatca gcgtcaacgg gtcggtggct gcgctggggc cggtggtcgt
     1381 cgtggtcgcc attcgagccg ggatcgcgcc cagtcaactg ctcattccac tcgccttcgc
     1441 tgcccacgcc ggctccttgc tgctgctgac cggaaccccg gtcaacattc tggtcagcga
     1501 tgcggcggcc gacgcgggcg cgcgcccgtt cggcttcttc gagttcgcgc tggcgggcct
     1561 gccgctgctg gccgggactc tgctgatcat cgtgctgttc ggtcagcggc tgctgccaca
     1621 ccggccgccg agcgccatgt cgccggacgt cagtgagcac gtccggattc tgcaggcaca
     1681 gtacgaactg cccaccgaca cccagttggt gggagctcgg cgaggggtca ctgaggtcgt
     1741 catcccgccg cgctccgagc tgatcggcct gcgcgtgttc cccggcatga gcacaccctc
     1801 gggcgacctc gtggtgctgg gcctgcagcg ggcgggggag ccgctcggcg gctccgacac
     1861 cacgctcgag accggtgacg tgatgctgct cggcggcacc tggcagcacc tggagcacca
     1921 tacgtccggc tccgaggttc tggtggtgga ccccccggac gcgctgcggc gttccattcc
     1981 gctcggtgcg ggcgccaagc gttcgatcgc ggtgctcgcg gcgatggtgc tgctgctcgc
     2041 caccggcctg gtgccgccgg ccatcgcggc ggtgctggcc gctctggcgc tcgtcctgac
     2101 cggtgtgcgc acgtcagccg aggcctaccg cgccgtcaac tggacgacgg tggtgctggt
     2161 cggcggcatg attccgctct cgaccgcgtt catctccacc ggggccgccg atctgatcgc
     2221 cgagggtgtg ctcaccatgg tcgggggcag ctcgccgtat ctggcgctgc tggtgatgtg
     2281 cgtgctgacc atgattcttg gccagttgat cagcaacatg gccacggtgc tgatcatgtt
     2341 gccgatcgcc acggtgttgg ccgccgacct gcacgtgtcg gtgctgccat tcatgatggc
     2401 cctgaccgtg gcgggggcgg cggcctttct caccccggtg gcgacgccgg ccaacacgat
     2461 ggtgttcgag cccggcggct acgtgttctc tgactactgg aagctcggcc tgccgattct
     2521 gttgctgttc ctggccgtcg cggtgttctg ggtgccgttc gtctggccgt tctgaatccg
     2581 gagctggccc cactcagccc gtgcccgtcc ttccgcttga ctgcgcgtga acggagttga
     2641 gaaccgtccc cgactccgtt caccactccg cccgtgcccg tccttccgct gtgagctgcg
     2701 ggtgaacgca gggggaagtc gtccccgact ccgttcgcga ccgctcggac cattccatgc
     2761 ccctcccgcg acctgcccgt gtcaacgatg ttgtgcagca gcctgtcatt cgtcaccgtc
     2821 gccaggccct gccggtcagc ggtttggcga agcgccggcc agcgcgctat ttttacatca
     2881 tctaatgtaa aaaatgagga ggccgtggtg aggcggggac gttcgccctg gcgggactgg
     2941 ccgctgatcg gctggctgag catcgccgtg gctgtcgctc tcggccaccg gtggctggcc
     3001 gagagccagt ggctgctgct gcacctggtg tttctcgggg ccgtcaccca ttcgatcatg
     3061 gtctggagca tgcacttcgc gcagaccctg ctgcgtcagc caccgccagc gcatcaggac
     3121 ctgcgactgg ccctgctgac gctcggctcg gctttggtgt tcatcggtgt gcccaccgcc
     3181 aactggtggc tcacgctcgt cggcggcagc atcgtcgcgg tcaccgtggg ctggcacgcc
     3241 tggctgctgc tgcgcatggt gcgcaccgcc ctgcccgccc ggtttcgtgt caccgtgcgg
     3301 tactacatcg ccgccggttg cagcctcgcc ctcggggccg gcttcggcat cgccttggcc
     3361 tggggctgga cggacagctg gcacggacgc ctgctggtcg cgcacgccct gacgaatgtg
     3421 ctgggctggc tgggcctcac cgtgaccgga accctgctca ccctgtggcc gaccatgctg
     3481 cgcacccgca tggacgcctc cgccgaggcg ctgaccgtcc gcgcactacc ggtgcttctc
     3541 accggcatcg cggtggcggt gggtggtgct ctgatcggcg tcacctggat cggtgccctc
     3601 ggcctggtcg cctacctggc cgggttcgcg ctgtgggccg gggcgttgtg gcgtccgctt
     3661 cgggccaagc cgatcaccga gttcgccccc gcatcggtcg ccgccgccct gtgctggctg
     3721 gtgatcgggc tgacctgggc gggttgggtg ctggtcagcg ccggcgactg ggtgcaggtg
     3781 cgtgagcagt tcatctggcc cgcagccgcg ctggccggcg ggttcggtgt gcagatcctg
     3841 accggcgcgc tgtcgtacct gctgccgtcc gtgctgggcg gagggccgtc cgtggtgcgc
     3901 gccgggcagc gcttcttcaa cgccgccgcc ggttttcgcc tggtcgccgt caacggtggt
     3961 ctggtgctgt ggctcgcgcc gacaccgagt tgggtgaagg tgaccggctc gctgctggcg
     4021 ctgggcgccg cggcctcgtt tctgccgatt ctggtgctgg ggctgaaggc gtccatcgct
     4081 gcccggcgtg cggtggccaa gggcgagcaa cccgagccgc ccaccgagcg tccctcaccg
     4141 ttcgcggcga cccaactgat cgcgggtctc accgcggtgg ccgtcgcggt cgtggtcggc
     4201 gtgggggtgg atccgcacgc cgccggcgtg gccgccgccc ccgccgccac tcaggtggcc
     4261 ccgaccggac ggacggtgcg cgtgagcgtc gagatcgtcg ccatgcacta cgtgcccagt
     4321 tcggtgagcg tgcatcccgg agacaccctg gtggtcgacc tggtcaactc cgacaccaac
     4381 acccacgacc tggtgatcgg cgaggctcgg acggcccggc tcgggcccgg cgagaaggcc
     4441 accctggacg ccggcgtgat cggcgccacg gtgcagggct actgctcgat cgtcggccac
     4501 aagcagatgg gcatgacctt cgacgtcaac gtggtgggtg gtccggcgac cagcccgacg
     4561 gcgtccgctg cgccggacgg gcatgccggc cacgactcgt ccaccgggac gcagccgctc
     4621 tcggcagtga tcgatccggt gttggccccg ctcagcgccg agaaggtgca cagactcaca
     4681 ctgaccgcgc aggagcttcc gctcgaggtg gcacccgggg tgtggcagcg gcgctggacc
     4741 ttcaacggtc gagcgccggg cccgaccctg cacggccggg tcggcgacgt gttcgagatc
     4801 accctggtca acggcggcac gatcgggcac tcgatcgact ttcacgccgg atcgctcgca
     4861 cccgatcgcc cgatgcgcac gatccagccc ggtgagtcac tggtgtaccg gttcaccgcg
     4921 accaaggccg gggtctggct gtaccattgc tcgacgatgc cgatgtcgac ccacatcgcg
     4981 gccggcatgt tcggtgcggt ggtgatcgaa ccggacgggt tgcccaccgt ggaccgctcc
     5041 tacctgctgg tgcagtcgga gacctacctg cagacccggg cccccgacgc ggtctcggcg
     5101 accgaggtga acgcggacgc gatcgcggcc gagcagccca gctacgtggc gttcaacggt
     5161 gtggcgaacg ggtacgacca ggtgccgctc gaggcgacgg cgggggagcg ggtgcggttc
     5221 tgggtgctgg acgccggccc gaatcgtccg agcagctttc acatcgtcgg tgcgcagttc
     5281 gacacggtct acgtcgaggg tcactacgag ttgcgccgcg gcaaggacgg cttcggcggc
     5341 accgacggag gatcgcaggc cctgggccta caggccgccc agggtggctt cgtcgaggcg
     5401 gtcttccacg aagccggccg ctacccgatg gtcacccacg tgatgagcga cgccgaacga
     5461 ggcgcccacg gctacgtggt ggtgcgccca tgaccgcgac acgccaccgt gcgagcccgc
     5521 atcgcccgtc ccaagaaccg caaccacaag gagaaacaat gaatcagtct gttcccatcg
     5581 aaaccatcga gatcgccgag gcgtccggac acacctgcaa ctgcggcgag catggcgcgg
     5641 ctgccccggt gctggacgtg cggcagattc cgcacgcgat ccggcacggt gcggtcttcg
     5701 gggcgctcga gcaactgggt gccggaggcg tgctggcact cgtggccccg cacaacccac
     5761 tgccgctgct gaaccaactc gcgcagcggt ggccgatcga catcgcctac ctcgaggacg
     5821 gtccggcggc ctggcagctt cggatcacgc gccgggccta agcctggatc caccgatctg
     5881 cgcctactgc gcggccccat ccgggcaccc tggctgcgtc ggccacgctc accagacgcc
     5941 cggcatgcct tcgcgcggac cggcttgcca ggccacccgt ctggtgccgc tcgcgacgg
//
LOCUS       DAOSUG010000137         5968 bp    DNA     linear   ENV 10-JUN-2024
DEFINITION  MAG TPA_asm: Micropruina sp. isolate AMR_MDS_2195
            NODE_6182_length_5968_cov_4.090479, whole genome shotgun sequence.
ACCESSION   DAOSUG010000137 DAOSUG010000000
VERSION     DAOSUG010000137.1
DBLINK      BioProject: PRJNA925477
            BioSample: SAMN32864262
            Sequence Read Archive: SRR4236673
KEYWORDS    WGS; ENV; Metagenome Assembled Genome; MAG; Third Party Data; TPA;
            TPA:assembly.
SOURCE      Micropruina sp. (wastewater metagenome)
  ORGANISM  Micropruina sp.
            Bacteria; Bacillati; Actinomycetota; Actinomycetes;
            Propionibacteriales; Nocardioidaceae; Micropruina.
REFERENCE   1  (bases 1 to 5968)
  AUTHORS   Abdulkadir,N., Saraiva,J.P., Zhang,J., Stolte,S., Gillor,O.,
            Harms,H. and Rocha,U.
  TITLE     Genome-centric analyses of 165 metagenomes show that mobile genetic
            elements are crucial for the transmission of antimicrobial
            resistance genes to pathogens in activated sludge and wastewater
  JOURNAL   Microbiol Spectr 12 (3), e0291823 (2024)
   PUBMED   38289113
REFERENCE   2  (bases 1 to 5968)
  AUTHORS   Abdulkadir,N.
  TITLE     Direct Submission
  JOURNAL   Submitted (24-MAR-2023) Environmental Microbiology, Helmholtz
            Center for Environmental Research -UFZ, Permoserstr 15, Leipzig
            04318, Germany
COMMENT     The annotation was added by the NCBI Prokaryotic Genome Annotation
            Pipeline (PGAP). Information about PGAP can be found here:
            https://www.ncbi.nlm.nih.gov/genome/annotation_prok/
            
            ##Genome-Assembly-Data-START##
            Assembly Method        :: SPAdes v. 3.11.1
            Genome Representation  :: Full
            Expected Final Version :: Yes
            Genome Coverage        :: Not Applicable
            Sequencing Technology  :: Illumina MiSeq
            ##Genome-Assembly-Data-END##
            
            ##Genome-Annotation-Data-START##
            Annotation Provider               :: NCBI
            Annotation Date                   :: 05/04/2023 04:57:10
            Annotation Pipeline               :: NCBI Prokaryotic Genome
                                                 Annotation Pipeline (PGAP)
            Annotation Method                 :: Best-placed reference protein
                                                 set; GeneMarkS-2+
            Annotation Software revision      :: 6.5
            Features Annotated                :: Gene; CDS; rRNA; tRNA; ncRNA
            Genes (total)                     :: 2,776
            CDSs (total)                      :: 2,750
            Genes (coding)                    :: 2,714
            CDSs (with protein)               :: 2,714
            Genes (RNA)                       :: 26
            tRNAs                             :: 24
            ncRNAs                            :: 2
            Pseudo Genes (total)              :: 36
            CDSs (without protein)            :: 36
            Pseudo Genes (ambiguous residues) :: 0 of 36
            Pseudo Genes (frameshifted)       :: 2 of 36
            Pseudo Genes (incomplete)         :: 32 of 36
            Pseudo Genes (internal stop)      :: 2 of 36
            ##Genome-Annotation-Data-END##
FEATURES             Location/Qualifiers
     source          1..5968
                     /organism="Micropruina sp."
                     /mol_type="genomic DNA"
                     /submitter_seqid="NODE_6182_length_5968_cov_4.090479"
                     /isolate="AMR_MDS_2195"
                     /isolation_source="Activated sludge of municipal
                     wastewater treatment plants"
                     /db_xref="taxon:2737536"
                     /environmental_sample
                     /geo_loc_name="Canada"
                     /lat_lon="49.25 N 123.1 W"
                     /collection_date="2015-06-16"
                     /metagenome_source="wastewater metagenome"
                     /note="metagenomic"
     gene            15..1433
                     /locus_tag="PLF56_05900"
     CDS             15..1433
                     /locus_tag="PLF56_05900"
                     /EC_number="2.3.1.-"
                     /inference="COORDINATES: protein motif:HMM:NF012424.2"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_process: GO:0006096 - glycolytic process [Evidence
                     IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="dihydrolipoamide acetyltransferase family
                     protein"
                     /protein_id="HOQ53131.1"
                     /translation="MASLLRMPGVSADADTALLESWSIAAGDTVSRGDVLATVETDKA
                     MVEIEAESDAVVHTLLVDGGSTVAVGDPIAVLAEPGEDPAAAAALAAQLTGSAPAPEP
                     EPEPAVALETIAVAEDMQAPDPGAPAAPAPPVTTPDAPGSVPAPRPTRRPGERIFSSP
                     LARRLAAEYGLELADIDGTGPNNRITKKDVESAYADAAGQVAPPVTGSRQARPMQEAR
                     PTEQARPAQGTRPAQPAQASRPARPAQGSVTHIPHTRLRRAIAGRLQSSKQNAPHFYL
                     TRELDLGPLLALRAQVNEGGEVRVSVNDLLVKAAGLALQQVPEANVSYATDELLQYGS
                     SDVAVAVASDRGLVTPVIRSVESLGLTALSAAIKDAAKRAGEGRLQQKELEGGSLTVS
                     NLGMFDVDEFAAILNPPQSTILAIGSGKPRPWVDASGQLTVATTCRVTASFDHRAIDG
                     ALGAQYLQAFSALIARPIQLLL"
     gene            1485..2486
                     /gene="dhaK"
                     /locus_tag="PLF56_05905"
     CDS             1485..2486
                     /gene="dhaK"
                     /locus_tag="PLF56_05905"
                     /EC_number="2.7.1.121"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_012924206.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_function: GO:0004371 - glycerone kinase activity
                     [Evidence IEA];
                     GO_process: GO:0006071 - glycerol metabolic process
                     [Evidence IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="dihydroxyacetone kinase subunit DhaK"
                     /protein_id="HOQ53132.1"
                     /translation="MKKIINDPSTVLDEMNRGFATAYADIVELHDDPLYVCRKGGAVQ
                     GKVGVISGGGSGHEPLGGGYVGEGMLSAAAPGAFFSSPTPDQILAATQAADGGAGVLH
                     LILNYTGDILNFETGAELAELEEIPVKTVVIDDDVAVENSTYTAGRRGVAGAIVAFKL
                     AGAAADRGDDLENVTRIAAKANDNMATMGLAMTACTVPTVGKPSFELGEDEMELGIGV
                     HGEPGVKRVPMMKADDIVDLLLEKVLDDLKLESGENVLLYINGLGATPLSEQYILYNH
                     AHDVLTAKGINVIRSQIGNFFTSLEMSGASLTVMRLDDELTELYDAPVRTAGWRAGA"
     gene            2562..3821
                     /locus_tag="PLF56_05910"
     CDS             2562..3821
                     /locus_tag="PLF56_05910"
                     /inference="COORDINATES: protein motif:HMM:NF013740.2"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_function: GO:0015562 - efflux transmembrane transporter
                     activity [Evidence IEA];
                     GO_process: GO:1905887 - autoinducer AI-2 transmembrane
                     transport [Evidence IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="AI-2E family transporter"
                     /protein_id="HOQ53133.1"
                     /translation="MSTQLSPEQLRAPEGIRILYAGAAIVACLVGVYFIHDVVAPVFL
                     AITLVLTVRPLVVWLDGHRVPKILGSLIGMLVVFLVLLAILFAVTVAMIQFVQAMPAY
                     ADRFNQIYNSVYQQMIAWGISETTITEWLRSFDYTRLLGYVTGVMSSVQFWATFVGSL
                     FLFVAFVAVDMTDTTEPRSKLAVVKPNLAAALLDFSWRVRRYWIVNTIFGLCVALINM
                     GILAWLQIPLVLTWGILAFVTAYIPNVGFLIGMIPPALMALLARDVQTMLIMVGLYVV
                     VNFIASMVIQPKVTGDAVGLNITTTFISLVFWAIVIGPLGPILAVPLTLFCKSVFFDA
                     YPRTRWVSVFMGGNLKELGGAQPVPPLESPAVQDDPLVEPEPDAHTHAQPASDDDADA
                     EPASEPVAETATGSEPLAVSENKSPPA"
     gene            3930..5840
                     /locus_tag="PLF56_05915"
     CDS             3930..5840
                     /locus_tag="PLF56_05915"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_020576001.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_function: GO:0016627 - oxidoreductase activity, acting
                     on the CH-CH group of donors [Evidence IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="acyl-CoA dehydrogenase family protein"
                     /protein_id="HOQ53134.1"
                     /translation="MSETKAGPTEREARKVAEAARETRWTRPSFGKELFLGRLRLDLV
                     DPWPSNSPEQVETGEAYLARLRPFVEGIDGTAIERDASISDEVFTGLAELGAFGMKVP
                     QQYGGVGLSHLYYCKALTLTGSAHASVGTLLSAHQSIGVPQPVKMFGTDEQKQRYLPR
                     VAGGEVSAFLLTEPDVGSDPARLACKARPVEGGYLLNGVKLWATNGTVATLLVVMARV
                     PASEGRRGGVTAFVVESNSEGITVENRNAFMGLRGIENSVTRFHDVFVPEANVLGGEG
                     RGLKIALTTLNTGRLSLPAMCVASSKWCLNVARGWAAERVQWGRPVGRHEALSTKISF
                     IAATTYALEAMVDLTCLLADDDENDIRIEAALLKLYASEMAWKVADELVQIRGGRGFE
                     TADSQRARGERGIEAEQVLRDLRIMRIFEGSTEIMHLLIAREAVDAHLDVAGDIIDPE
                     ASRDDKLKAVGKAGVFYAGWLPTLVAGKGLVPTSFGEFGPLATHLRFIERTTRKLARS
                     IFYAMARWQGAMERKQAFLGRVVDIGAELFAMTAACGRAKAERATHPQGIQLADLFCA
                     QARVRIDALFTALWSNTDDADDAAAKQVLEGRYAFVESGIKLPSDEGPWVSPVERGPS
                     VRPDVRRVIPRP"
BASE COUNT          949 a         2052 c         2028 g          939 t
ORIGIN      
        1 gaagggatga cctgatggcc tcgcttcttc gcatgcccgg cgtgtccgcc gacgccgaca
       61 ccgccctgct cgagagctgg tcgatcgccg cgggcgacac ggtcagccgc ggcgacgtgc
      121 tggccaccgt cgaaaccgac aaggcgatgg tcgagatcga ggccgaatcg gacgcggtgg
      181 tgcacaccct gctcgttgac ggcggctcga ccgttgccgt gggcgacccg atcgccgtgc
      241 tggccgagcc gggggaggac ccggcggccg ccgccgcgct ggcggcgcaa ctgaccggct
      301 cggctccggc accagaaccc gagccggagc cagcggtggc ccttgaaacc atcgccgtgg
      361 ccgaagacat gcaggcgccc gaccccgggg cgcccgccgc ccccgcgccg ccggtcacga
      421 cgccggacgc gccgggttcg gtccccgctc cgcgcccgac ccgtcgtccg ggtgaacgca
      481 tcttctccag cccgctggcc cgccggttgg ccgccgagta cggcctggag ttggccgaca
      541 tcgacggcac cggaccgaac aaccggatca ccaagaagga cgtggagtcg gcctacgccg
      601 atgcggcggg tcaggttgcg ccacccgtca ccggatctcg acaggcccgc ccgatgcagg
      661 aagcccgccc gacggagcaa gctcgaccgg cgcaggggac gcgaccagcc caaccagcgc
      721 aggcgtctcg accagctcga ccagcgcagg gatccgtgac ccacattccg cacacccggc
      781 tgcgccgggc gatcgccggc cgcctgcaga gttccaagca gaacgcaccg cacttctacc
      841 tcacccgcga actcgacctc ggcccgctgc ttgcgctgcg cgcccaggtc aacgagggcg
      901 gcgaggtgcg ggtgtcggtc aacgacctgc tggtcaaggc cgccggcctg gcgttgcagc
      961 aggtgccgga ggccaacgtc agctatgcca ccgacgagtt gctacagtac ggcagcagcg
     1021 acgtggcggt ggcggtggcc agcgaccgcg gcctggtcac cccggtgatc cgcagcgtcg
     1081 aaagcctcgg cctgaccgca ctctcggccg ccatcaagga cgccgcgaag cgggccggcg
     1141 aaggccggct gcagcagaag gaactcgagg gcggctcgct gacggtgagc aacctcggca
     1201 tgttcgacgt cgacgagttc gccgccattc tgaatccgcc gcagagcacg atcctggcga
     1261 tcggctccgg caagccacgt ccgtgggtgg acgcctccgg gcaactcacg gtcgccacca
     1321 cctgccgggt cacggcgtcc ttcgaccacc gcgccatcga cggcgcgctg ggggcgcagt
     1381 acctgcaggc cttctccgcc ctgatcgccc gccccatcca actactgctg tgatccttca
     1441 cccgttcggc gcaacgctgc gcccatgtcc ttgaggagag gcacatgaag aagatcatca
     1501 acgacccgtc caccgttctc gacgagatga accgcggctt cgccacggcc tacgccgaca
     1561 tcgtcgagtt gcacgacgac ccgttgtacg tgtgccgcaa gggcggtgcc gtgcagggca
     1621 aggtgggcgt gatctcgggc ggcggctcgg gccacgagcc cctcggcggc ggctacgtcg
     1681 gcgagggcat gctgagcgcc gcggcgccgg gtgcgttctt cagctcgccg acccccgacc
     1741 agatcctcgc cgccacccag gcagcggacg gcggcgcggg cgtcctgcac ctgatcctca
     1801 actacaccgg cgacatcctg aacttcgaga ccggcgccga actggccgaa ctcgaagaga
     1861 tcccggtcaa gacggtggtg atcgacgacg acgtcgccgt cgagaactcc acctacaccg
     1921 ccggacgccg cggcgtggcg ggcgccatcg tggcgttcaa gctggccggt gccgccgccg
     1981 atcgcggcga cgacctcgag aacgtcaccc ggatcgcggc caaggccaac gacaacatgg
     2041 ccaccatggg cctggcgatg accgcctgca cggtgccgac cgtcggcaag ccgagcttcg
     2101 aactcggtga ggacgagatg gaactcggca tcggcgtgca cggcgagccg ggcgtcaagc
     2161 gggtgccgat gatgaaggcc gacgacatcg tcgaccttct gctcgagaaa gtgctggacg
     2221 acctgaagct cgagagcggc gagaacgtgc tgctgtacat caacggcctg ggcgccacgc
     2281 cgctgagcga gcagtacatc ctgtacaacc acgcgcacga cgtgctgacc gcgaagggta
     2341 tcaacgtcat ccgtagccag atcggcaact tcttcacctc gctcgagatg tcgggtgcct
     2401 cgctgaccgt gatgcggctg gacgacgagc tcaccgagct gtacgacgcc ccggtgcgca
     2461 cagcgggctg gcgcgcgggc gcctgagcct gggtctggac gggctcacct tacggggtga
     2521 gtccgtccga ttctgtctgc gatcggatct agactccccg cgtgagcacg caactcagcc
     2581 cggagcagct tcgggcaccc gagggcatcc gcattctgta tgcaggtgcg gcgatcgtgg
     2641 cctgcctggt cggggtctac ttcatccacg acgtggtggc accggtcttc ttggcgatca
     2701 cgctcgtgct gaccgtgcgt ccgctggtgg tgtggctcga cggccaccgg gtgcccaaga
     2761 tactgggctc gctgatcggc atgctcgtgg tgttcctggt gctgctggcg attctgttcg
     2821 cggtcaccgt cgcgatgatc cagttcgtgc aggcaatgcc ggcctacgca gatcggttca
     2881 accagatcta caacagcgtg taccagcaga tgatcgcctg gggcatcagc gagaccacga
     2941 tcaccgagtg gttgcggtcc ttcgactaca ccagattgct cggctacgtc acgggcgtga
     3001 tgagttcggt gcagttctgg gccaccttcg tcggctcgct gttcctgttc gtcgctttcg
     3061 tggcggtcga catgaccgac accaccgagc cgcgcagcaa gctcgccgtg gtcaagccca
     3121 acctggcggc cgcgctgctc gacttctcgt ggcgggtgcg tcggtactgg atcgtcaaca
     3181 cgatcttcgg cctgtgcgtg gcgctgatca acatgggcat tctggcctgg ttgcagattc
     3241 ccctggtgct cacctggggc attctcgcct tcgtcaccgc ctacattccc aacgtcggct
     3301 ttctgatcgg catgatcccg cccgccctga tggcactgct ggctcgtgac gtacagacca
     3361 tgctgatcat ggtgggcctg tacgtggtgg tgaacttcat cgcctcaatg gtgattcagc
     3421 ccaaggtgac cggtgacgcg gtggggttga acatcaccac cacgttcatc tcactggtgt
     3481 tctgggcgat cgtcatcggt ccgctcggcc cgattctcgc cgtgccgctc acgctgttct
     3541 gcaagagcgt cttcttcgac gcctacccgc gcacccgctg ggtgtcggtg ttcatgggcg
     3601 gcaacctcaa ggagttgggc ggcgcccaac ccgtgccgcc gctggagtcg ccggccgtgc
     3661 aggacgatcc gttggtcgag cccgagcccg acgcccacac tcacgctcag cccgcgagtg
     3721 acgacgatgc tgatgccgag cccgcgtccg aacccgtcgc tgagacggcc actgggtccg
     3781 agccgctcgc agtgagcgag aacaagtcgc cgccggcctg accgcacgac gccggctccg
     3841 gcgaagtccg ggtcacttcg ttgtgccacg cgtcctgttg tgcagggcct cggtgaacta
     3901 ccgtcgaggc agagcacgag gaggtgcgca tgtctgagac caaggccggc cccaccgaac
     3961 gcgaggcccg caaggtcgcc gaggccgccc gcgagacccg ctggaccagg cccagcttcg
     4021 gcaaggaact ctttctgggc cggctgcggc tcgacctggt cgacccgtgg ccgagcaaca
     4081 gccccgagca ggtcgagacg ggggaggcgt acctggcgcg actgcgtccg ttcgtggagg
     4141 gcatcgacgg cacggcgatc gagcgcgacg cgtccatctc ggacgaggtg ttcaccggcc
     4201 tggccgaact cggcgccttc ggcatgaagg ttccccagca gtacggcggg gtcgggctgt
     4261 cgcacctgta ctactgcaag gcgctcaccc tgaccggttc cgcgcacgcc tcggtcggca
     4321 cgctgctcag tgcacaccag tcgatcgggg tgccgcagcc ggtgaagatg ttcggcaccg
     4381 acgagcagaa acagcggtac ctgccccggg tggccggcgg cgaggtgtcg gcctttctgc
     4441 tcaccgagcc ggacgtcggt agcgacccgg cccggctggc ctgcaaggct cgtccggtcg
     4501 agggcggcta cctgctgaac ggggtcaagc tgtgggccac caacggcacg gtcgcgacgc
     4561 tgctggtggt gatggcacgg gtgcccgcga gcgaaggccg gcgcggcggc gtcaccgcgt
     4621 tcgtggtcga gtcgaactcc gagggcatca cggtcgagaa ccgcaacgcg ttcatggggc
     4681 tgcgcggcat cgagaactcg gtcacccggt ttcacgacgt gttcgtgccc gaggccaacg
     4741 tgctgggcgg tgagggacgg ggcctcaaga tcgccctgac caccctgaac accggacgcc
     4801 tgtcgctgcc ggcgatgtgc gtggcctcgt ccaagtggtg cctgaacgtg gcccgcggct
     4861 gggcggccga acgggtgcag tggggtcgtc cggtcggccg gcacgaggca ctgtcgacga
     4921 agatcagctt catcgccgcc accacctatg cgctggaggc gatggtcgac ctgacctgcc
     4981 tgctggccga cgacgacgag aacgacattc gcatcgaggc ggcactgctc aagctgtacg
     5041 cgtccgagat ggcgtggaag gtggcggacg agctggtgca gattcgcggc ggccgcggct
     5101 tcgagaccgc cgattcgcag cgggcccgcg gcgagcgggg catcgaggcc gagcaggtgc
     5161 tgcgcgacct gcgcatcatg cgcatcttcg agggctccac cgagatcatg cacctgctga
     5221 tcgcccgcga ggccgtcgat gcccacctcg acgtggccgg cgacatcatc gaccccgagg
     5281 cgtcccgcga cgacaagctc aaggcggtgg gcaaggcggg cgtcttctat gcaggctggt
     5341 tgcccaccct ggtggcgggc aagggcctgg tgcccacgtc gttcggcgag ttcggaccgc
     5401 tggccacgca cctgcgcttc atcgagcgca ccacccgcaa gctggcccgc tcgatcttct
     5461 acgcaatggc gcgatggcaa ggtgcgatgg agcgtaagca ggcctttctc ggccgggtgg
     5521 tcgacatcgg tgccgagctt ttcgcgatga ccgcggcctg cggacgggcc aaggccgagc
     5581 gtgcgacgca cccgcagggc atccagttgg ccgacctgtt ctgcgcccag gcccgggtgc
     5641 gcatcgacgc gctgttcacc gcgctgtgga gcaacaccga cgatgcggac gatgccgccg
     5701 ccaagcaggt gctcgagggc cgctacgcgt tcgtggagtc gggcatcaag ctgccctcgg
     5761 acgaggggcc gtgggtgagc ccggtcgagc ggggtccatc ggtcaggccg gatgtgcggc
     5821 gggtgatccc ccggccgtag atagagacca ccccgttcga gctcgtcggt accgccgctt
     5881 gtcgagctcg tcgaaacctg gtgcacccgg ctgtcgagct cgtcgaaacc gggtgcacct
     5941 gctgctccag tccaccggta cccgtccg
//
LOCUS       DAOSUG010000138         5966 bp    DNA     linear   ENV 10-JUN-2024
DEFINITION  MAG TPA_asm: Micropruina sp. isolate AMR_MDS_2195
            NODE_6184_length_5966_cov_3.866013, whole genome shotgun sequence.
ACCESSION   DAOSUG010000138 DAOSUG010000000
VERSION     DAOSUG010000138.1
DBLINK      BioProject: PRJNA925477
            BioSample: SAMN32864262
            Sequence Read Archive: SRR4236673
KEYWORDS    WGS; ENV; Metagenome Assembled Genome; MAG; Third Party Data; TPA;
            TPA:assembly.
SOURCE      Micropruina sp. (wastewater metagenome)
  ORGANISM  Micropruina sp.
            Bacteria; Bacillati; Actinomycetota; Actinomycetes;
            Propionibacteriales; Nocardioidaceae; Micropruina.
REFERENCE   1  (bases 1 to 5966)
  AUTHORS   Abdulkadir,N., Saraiva,J.P., Zhang,J., Stolte,S., Gillor,O.,
            Harms,H. and Rocha,U.
  TITLE     Genome-centric analyses of 165 metagenomes show that mobile genetic
            elements are crucial for the transmission of antimicrobial
            resistance genes to pathogens in activated sludge and wastewater
  JOURNAL   Microbiol Spectr 12 (3), e0291823 (2024)
   PUBMED   38289113
REFERENCE   2  (bases 1 to 5966)
  AUTHORS   Abdulkadir,N.
  TITLE     Direct Submission
  JOURNAL   Submitted (24-MAR-2023) Environmental Microbiology, Helmholtz
            Center for Environmental Research -UFZ, Permoserstr 15, Leipzig
            04318, Germany
COMMENT     The annotation was added by the NCBI Prokaryotic Genome Annotation
            Pipeline (PGAP). Information about PGAP can be found here:
            https://www.ncbi.nlm.nih.gov/genome/annotation_prok/
            
            ##Genome-Assembly-Data-START##
            Assembly Method        :: SPAdes v. 3.11.1
            Genome Representation  :: Full
            Expected Final Version :: Yes
            Genome Coverage        :: Not Applicable
            Sequencing Technology  :: Illumina MiSeq
            ##Genome-Assembly-Data-END##
            
            ##Genome-Annotation-Data-START##
            Annotation Provider               :: NCBI
            Annotation Date                   :: 05/04/2023 04:57:10
            Annotation Pipeline               :: NCBI Prokaryotic Genome
                                                 Annotation Pipeline (PGAP)
            Annotation Method                 :: Best-placed reference protein
                                                 set; GeneMarkS-2+
            Annotation Software revision      :: 6.5
            Features Annotated                :: Gene; CDS; rRNA; tRNA; ncRNA
            Genes (total)                     :: 2,776
            CDSs (total)                      :: 2,750
            Genes (coding)                    :: 2,714
            CDSs (with protein)               :: 2,714
            Genes (RNA)                       :: 26
            tRNAs                             :: 24
            ncRNAs                            :: 2
            Pseudo Genes (total)              :: 36
            CDSs (without protein)            :: 36
            Pseudo Genes (ambiguous residues) :: 0 of 36
            Pseudo Genes (frameshifted)       :: 2 of 36
            Pseudo Genes (incomplete)         :: 32 of 36
            Pseudo Genes (internal stop)      :: 2 of 36
            ##Genome-Annotation-Data-END##
FEATURES             Location/Qualifiers
     source          1..5966
                     /organism="Micropruina sp."
                     /mol_type="genomic DNA"
                     /submitter_seqid="NODE_6184_length_5966_cov_3.866013"
                     /isolate="AMR_MDS_2195"
                     /isolation_source="Activated sludge of municipal
                     wastewater treatment plants"
                     /db_xref="taxon:2737536"
                     /environmental_sample
                     /geo_loc_name="Canada"
                     /lat_lon="49.25 N 123.1 W"
                     /collection_date="2015-06-16"
                     /metagenome_source="wastewater metagenome"
                     /note="metagenomic"
     gene            <1..1088
                     /gene="hflX"
                     /locus_tag="PLF56_05920"
     CDS             <1..1088
                     /gene="hflX"
                     /locus_tag="PLF56_05920"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_012922283.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_function: GO:0005525 - GTP binding [Evidence IEA];
                     GO_process: GO:0008150 - biological_process [Evidence
                     IEA]"
                     /codon_start=3
                     /transl_table=11
                     /product="GTPase HflX"
                     /protein_id="HOQ53135.1"
                     /translation="LREVVIATGADTVICDGELEPAQLRNLEDRVRVKVIDRTALILD
                     IFAQHAKSAEGKAQVELAQLQYLRQRLRGWGGNLSRQAGGRAAGGAGIGGRGPGETKI
                     ETDRRRIRTRISVLRKRLREMDEVRATKRQERQRNEVPSVAIVGYTNAGKSSLLNRLT
                     GAGVLVENALFATLDPTTRRSEASDGRVYTLTDTVGFVRHLPHDLVEAFRSTLEESVL
                     ADLLLHIVDGSDADPQGQIRAVRTVLGEIGAGDVPEQLVINKIDRAEPDALTVLRSQY
                     PDAVFVSARTGAGMDELKQRVEQRLPRPAIELRVLLPYDRGDLLNKIHAGGEILSLEH
                     TGDGTAVVARVNQGLAAELAEYQVVEG"
     gene            1168..1602
                     /locus_tag="PLF56_05925"
     CDS             1168..1602
                     /locus_tag="PLF56_05925"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_013865310.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="glyoxalase"
                     /protein_id="HOQ53136.1"
                     /translation="MTQMFVNLPVTDLERAKAFYTAVGFSINPLFTDHNAACVVVEDG
                     HSYFMLLVREFFQTFTDLPLGDPLVSPTTAVAIYVDSREAVDATVADGLSAGGSEDRP
                     PSDYGFMYQRQLADPDGNIIEFGWMDPAAATQGPEAFAGQQG"
     gene            1607..2248
                     /locus_tag="PLF56_05930"
     CDS             1607..2248
                     /locus_tag="PLF56_05930"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_013865309.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_function: GO:0003677 - DNA binding [Evidence IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="helix-turn-helix domain-containing protein"
                     /protein_id="HOQ53137.1"
                     /translation="MAARDYGQYCGITRALELVGERWALLIVRDLLVGPRRYGELAAG
                     LPRIPTNILAARLKELQEAGIIRRAPRSRIIVYELTPYGRELEPVVLALGAWGFKAMD
                     EPREGQIVTADSMTMALRTAFRPLVAAELPSTVYGARFGEAELLVGVDGPRLAVRPGV
                     GPVDLAFAAGPGIRRVISGELDPQQAIAQGVVDVLTGPPQLLVRFADTFHLAA"
     gene            2398..4332
                     /locus_tag="PLF56_05935"
     CDS             2398..4332
                     /locus_tag="PLF56_05935"
                     /EC_number="3.6.4.12"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_014846426.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_function: GO:0003677 - DNA binding [Evidence IEA];
                     GO_function: GO:0004386 - helicase activity [Evidence
                     IEA];
                     GO_function: GO:0005524 - ATP binding [Evidence IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="ATP-dependent DNA helicase"
                     /protein_id="HOQ53138.1"
                     /translation="MAAAVAETLAGGDHLLVQAGTGTGKSLGYLAPALAHLAQAPDDR
                     IVIATATLALQAQLARKDIPSALAACQQVTGRTVTSAVLKGRTNYACLYRARDGLGLD
                     QESLLGGAELAEALTATATDASSKVGAEVVALREWIETEAKAKRVADRDDAPSHGAQS
                     WAQVSIPVRECLGVQRCPYGQECFVERSRDRARAAQLVVTNHALLAIDAMHGGAALPE
                     HDAVIIDEAHELVSRVTGAASHELSPQLIERVARRCLPWLDDDLGLDFLDSGSALATA
                     LDDAPLDRVEDPDSGLGVALRLVRDVTRRVISALAGDESDNDRKQAAAAAKEVFDIAA
                     QLAAMAPHDVVWVSERERFGRQVNQAPLSVAGLMREGIFAKHPVVLTSATLSIGGNFE
                     AVAASVGLAEPYRTDGESDAEPELPHWRGLDVGSPFDYRRQGILYVAKSLPPPGRDGI
                     GPATLAEVAELLWAAGGRTLGLFASQRAAEAAALHCRRELPDLEILCQGDAQLGELTR
                     RFVATPETSLFGTLSLWQGVDVPGDTCRLVIIDKIPFPRPDEPLMQARQRAVAERGGN
                     GFMAVAATHAALLLAQGSGRLIRRLTDRGVVAVLDPRLATARYGSFLRASMPDFWPTN
                     DREQAIGALRRLGEADPAAS"
     gene            4575..5759
                     /locus_tag="PLF56_05940"
     CDS             4575..5759
                     /locus_tag="PLF56_05940"
                     /inference="COORDINATES: protein motif:HMM:NF014719.2"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_component: GO:0016020 - membrane [Evidence IEA];
                     GO_function: GO:0005436 - sodium:phosphate symporter
                     activity [Evidence IEA];
                     GO_function: GO:0015114 - phosphate ion transmembrane
                     transporter activity [Evidence IEA];
                     GO_process: GO:0035435 - phosphate ion transmembrane
                     transport [Evidence IEA];
                     GO_process: GO:0044341 - sodium-dependent phosphate
                     transport [Evidence IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="Na/Pi symporter"
                     /protein_id="HOQ53139.1"
                     /translation="MGTGRSDVDVVHVPPASGRSSTVLRWIAVVLSLYLLISAVSVIS
                     RGFRSLGAQQAEQLFAFAENPLVGLAVGILATVLIQSSTTTTAITVAAVGSGALDVTQ
                     AIPIILGANVGTTVTCTFVALGFAGNATEFRRALASSTIHDFYNLLALLIFFPLEVLF
                     HPLERMSGVLTDSLYGVTTPDPGDFNVVRMATRPLVNAVTSVTGLAGEGTTSAVLTIV
                     VGVAMIFLAVRWLSHFLKALMVGRTRELLLRAVEGTPAKAIGVGAGVTIVTQSSTVTN
                     SVLVPFAGTGTVTPGQLYPVTVGANLGTTLTALVAALAVNGPDAAVGLQAALVHVLYN
                     LLALLVVFVNPVLRPLPLKLASGLADLVAERRWLLAVYLVAVFVVVPSAIILLGALGK
                     LT"
BASE COUNT          862 a         2074 c         2072 g          958 t
ORIGIN      
        1 agctgcgcga ggtggtgatc gccaccggcg ccgacacggt gatctgcgac ggcgagctcg
       61 agcccgcgca gctgcgcaac ctggaggacc gggtgcgggt caaggtgatc gaccgcaccg
      121 ccctgatcct cgacatcttc gcccaacacg ccaagagtgc cgagggcaag gcgcaggtcg
      181 aactggccca gttgcagtac ctgcggcaac ggttgcgcgg ctggggcggc aacctgtccc
      241 ggcaggccgg cggacgggcg gccggtggcg ccggcatcgg tggccgcggc cccggcgaga
      301 ccaagatcga gaccgaccgg cgtcgcatcc gcacccgcat ctcggtgctg cgcaagcggc
      361 tgcgcgagat ggacgaggtg cgtgccacca agcgccagga gcggcaacgc aacgaggtgc
      421 cgtccgtggc catcgtcggg tacaccaacg ccggcaaatc ctcgctgctc aaccggctga
      481 ccggtgccgg cgtgctggtc gagaacgcgc tgttcgccac cctcgacccg accacgcggc
      541 gctccgaggc gtccgacgga cgggtgtaca cgctgaccga caccgtcggg ttcgtccgcc
      601 acctgccgca cgacctggtc gaggcgttcc gctccaccct ggaggagtcg gtgctggccg
      661 acctgctgct gcacatagtg gacggttccg acgccgaccc gcagggccag atccgggccg
      721 tccgcaccgt gttgggcgag atcggcgcgg gcgacgtgcc cgaacagctg gtgatcaaca
      781 agatcgaccg ggccgaaccc gacgcgctca cggtgctgcg ctcccaatac cccgacgccg
      841 tcttcgtctc ggcccgcacc ggcgcaggca tggacgaact gaagcagcgc gtcgagcagc
      901 ggctgccgcg tccggcgatc gaactgcggg tgctgctgcc ctacgaccgg ggcgacctgc
      961 tgaacaagat ccacgccggc ggcgagatcc tgagcctgga acacaccggt gacggcaccg
     1021 cggtggtggc ccgagtcaat cagggcctgg cggcggaact ggccgagtac caggtggtcg
     1081 agggctgatt ccacaccagc cattgaccgc tcacttgttt tttgcaagta tggcggtcaa
     1141 cgcaccgagc ctggaaagag gcccttgatg acccagatgt tcgtcaacct gccggtgacc
     1201 gacctggagc gcgcgaaggc gttctatacc gccgtcggtt tctccatcaa cccgcttttc
     1261 accgatcaca acgcggcctg cgtcgtggtg gaggacggcc acagctactt catgctgctg
     1321 gttcgcgagt tctttcagac cttcaccgac ctgccgctgg gcgacccgtt ggtgtccccg
     1381 acgacggccg tcgcgatcta cgtcgacagc cgcgaagcag tggacgccac agtggccgac
     1441 gggctctccg ccggcggctc cgaggatcgt ccgccctccg actacggctt catgtaccag
     1501 cgccagctgg ccgaccccga tggcaacatc atcgagttcg gctggatgga tccggccgcc
     1561 gcgacgcagg gccccgaagc gttcgccggc cagcaggggt gagcgcgtgg ccgctcgcga
     1621 ctacggccag tactgcggca tcacccgggc gctcgaactg gtgggggagc gctgggcgct
     1681 gctgatcgtc cgcgacctgc tcgtggggcc gcgccgctac ggggagttgg ccgccggtct
     1741 gccgcggatt ccgaccaaca ttctggcggc gcgactcaag gaactgcagg aggccggcat
     1801 catccgtcga gcgccgcgct cacgcatcat cgtgtacgag ctcaccccgt acggtcgcga
     1861 actcgaaccg gtggtgctgg ccctcggcgc ctggggcttc aaggccatgg acgagccgcg
     1921 cgagggccag atcgtgaccg ccgactcgat gaccatggca ttgcgcaccg ccttccgtcc
     1981 gctggtggcc gctgagctgc cgtccacggt gtacggtgcc cggttcggcg aggcggagct
     2041 gttggtagga gtcgatgggc cgcggctggc ggtgaggccc ggggtaggcc cggtcgacct
     2101 ggcctttgca gcgggcccgg gcatccgcag ggtgatctcc ggcgagctcg acccgcaaca
     2161 ggcgatcgct caaggcgtgg tcgacgtgct gaccggacct ccgcagcttc tcgtccgatt
     2221 cgccgacacc tttcacctgg ccgcctgagc tgagaccttg atcggggctg ggcgcacaaa
     2281 atcggtgtca gaggctcgcg gtaggttgtt tcggtgcccg agaccgatca gaccgaagcg
     2341 attctcgctg ccgcggtcga gggcatcgag ggtgcgcagc gtccgggtca gcacgagatg
     2401 gcggcggcgg tggccgaaac cctcgccggc ggcgaccatc tgttggtgca ggccggcact
     2461 ggcaccggta agtcgctggg gtacctggcg ccggctctcg cgcacctggc gcaggcaccc
     2521 gacgaccgca tcgtgatcgc cacggccacg ttggcgctgc aggcccagtt ggcccgaaaa
     2581 gacattccca gcgctctggc ggcgtgccag caggtcaccg gacgaaccgt cacctccgcc
     2641 gttctcaagg gccgcaccaa ctacgcctgc ctgtaccgcg cgcgcgacgg actcggcctc
     2701 gatcaggagt cgctgctggg cggggccgag ctggccgagg ccctgaccgc gacggccacc
     2761 gacgcctcgt ccaaggtcgg cgccgaggtg gtcgcgctgc gcgaatggat cgaaacggag
     2821 gcaaaggcga aacgcgtggc cgaccgcgat gacgccccca gccacggggc ccaatcatgg
     2881 gcgcaggtgt cgattccggt gcgcgagtgc ctcggggtgc agcgctgccc ctacggccag
     2941 gagtgtttcg tcgaacggtc ccgcgacagg gcgcgcgccg cgcagctggt ggtgaccaat
     3001 cacgcgctgc tggccatcga cgccatgcac ggcggcgccg cgctgcccga gcacgatgcg
     3061 gtgatcatcg acgaggcgca cgaactggtc agccgggtca ccggggcagc gtcacacgaa
     3121 ctcagcccgc aactgatcga gcgggtggcc cgccggtgcc tgccctggtt ggacgacgac
     3181 ctcggcctgg acttcctcga ctcgggctcc gcgctcgcca ccgctctgga cgacgccccg
     3241 ctggaccggg tggaagaccc cgacagcggc ctgggcgtgg ccctgcggct ggtgcgtgac
     3301 gtgacccggc gggtgatcag cgcgctggct ggcgacgagt ccgacaacga ccgcaagcag
     3361 gccgccgccg ccgcgaaaga ggtcttcgac atcgcagccc agttggccgc gatggctccg
     3421 cacgacgtgg tgtgggtctc ggagcgggaa cggttcggac gccaggtgaa ccaggcgccg
     3481 ctgtcagtgg ccgggctgat gcgcgagggc atcttcgcca agcatccggt cgtgctcacc
     3541 tcggccacgc tcagcatcgg cggcaacttc gaggcggtgg cggcctcggt cggtctcgcc
     3601 gagccgtacc gcaccgacgg cgagtccgac gccgaacccg agttgccgca ttggcgcggc
     3661 ctcgacgtgg gttcgccctt cgactaccgc cggcagggca tcctctacgt ggccaagtcg
     3721 ctgccgccac ccggccgcga cggtatcggt ccggccacgc tggccgaggt cgccgaactg
     3781 ctgtgggcgg ccggtggacg cacgctgggc ctgttcgcct cgcaacgcgc cgccgaagcg
     3841 gccgcgctgc actgccgccg cgaactgccc gacctcgaga ttctgtgcca gggcgacgca
     3901 caactcggtg aactcacccg gcggttcgtg gccaccccag agaccagcct cttcggcacc
     3961 ctgtcgttat ggcagggcgt cgatgtgccc ggcgacacct gcaggctggt gatcatcgac
     4021 aagatcccct tcccccgacc ggacgagccg ctgatgcagg cccgccagcg tgccgtcgcc
     4081 gagcggggcg gcaacggctt catggcggtg gcggccaccc acgcggcact gctgctggcc
     4141 cagggttccg gacggttgat ccgccggctc accgaccgtg gcgtcgtcgc ggtgctcgac
     4201 ccccggctgg cgacggcccg ctacggcagc tttctgcgtg cgtccatgcc cgacttctgg
     4261 ccgaccaacg accgcgagca ggcgatcggc gcgctgcgcc gcctcggtga ggccgacccc
     4321 gccgcatcat gaggcctgat cgcgcgctgc cccgggcgct gccgcgtgcc gcacgggggg
     4381 atcccgcagg tgtagtagtt gggctcgcag gtgcgtgagc ggaaatgcgt ccgagattca
     4441 ggcggtgccc gcgccaatgg ttcggcgaga gccgacagtt cgctcgggcg ttgcgtgccg
     4501 ttcaccgcac gtaacacggc tgtgcacagg tgcttcggac gggggggggg ggggggggga
     4561 gtagcgtgct gcccatgggc accggtaggt ccgacgttga cgtggtccac gtcccgccgg
     4621 cgtcaggccg atcgtccacc gtgctgcgct ggatcgcagt ggttctgtcc ctctacctgc
     4681 tcatctcagc tgtcagcgtc atctcgcgcg ggtttcgcag cctcggcgcc cagcaggccg
     4741 agcagctgtt cgccttcgct gagaaccccc tggtcgggct ggccgtcggc atcctggcca
     4801 cggtgttgat ccagtcgtcg accaccacca ccgccatcac ggtcgcagcg gtcgggtccg
     4861 gagccttgga tgtcacccag gccattccga tcattctggg tgccaacgtg ggcaccaccg
     4921 ttacctgcac cttcgtcgcg ctcggcttcg ccggtaacgc gaccgagttt cggcgggcgc
     4981 tggcaagctc cacgatccat gacttctaca acctgctcgc gctgctgatc ttctttccgc
     5041 tcgaggttct gtttcacccc cttgagcgaa tgtcgggggt gttgaccgac agcctctacg
     5101 gcgtcaccac acccgacccg ggtgacttca acgtggtccg catggcgacc cgcccgctgg
     5161 tgaacgccgt cacgtccgtg accggcttgg ccggtgaggg cacgacctcg gcggtgctca
     5221 cgatcgttgt cggtgtcgcg atgatctttc tggccgtgcg gtggttgagc cacttcctga
     5281 aggcgctgat ggtcggccgc acccgtgagc tgctgctgcg cgccgtcgag ggaacaccgg
     5341 cgaaagcgat cggcgttggc gcgggcgtca cgatcgtcac ccagtcgtcc actgtgacga
     5401 actcggtgct cgtgccgttc gcgggcacgg gtacggtcac ccccggccag ctgtacccgg
     5461 tgacggtcgg cgcgaacctt gggaccacgc tgaccgcttt ggtggcggca ctggcagtga
     5521 atgggcccga cgccgcggtc ggcctgcagg cggccttggt gcacgtgctg tacaacctgc
     5581 tcgccctgct ggtcgtgttc gtcaacccgg tgctgcgacc gctgccgctc aagctcgcct
     5641 cggggctggc cgacctggtg gccgagcggc gttggcttct tgccgtgtac ctggtggccg
     5701 tgttcgtggt ggtgccgagt gcgatcattc tgctcggtgc gctggggaag ctgacgtagc
     5761 cccgcctggt taccgctggt cgcgcccgaa ttgccgcgct gccgccgtcg atcactgcct
     5821 cgcgcgcccg gcccggccat tgtgggacgg ccgtgctcgg gtcgagatcc ggatttcgac
     5881 ggactccatg atgaagacgt gaccgtccgc cgccgggttt ctggtccgga gatcgagacc
     5941 cgctctcggt gtgcacccgc cgcgcc
//
LOCUS       DAOSUG010000139         5940 bp    DNA     linear   ENV 10-JUN-2024
DEFINITION  MAG TPA_asm: Micropruina sp. isolate AMR_MDS_2195
            NODE_6211_length_5940_cov_4.159218, whole genome shotgun sequence.
ACCESSION   DAOSUG010000139 DAOSUG010000000
VERSION     DAOSUG010000139.1
DBLINK      BioProject: PRJNA925477
            BioSample: SAMN32864262
            Sequence Read Archive: SRR4236673
KEYWORDS    WGS; ENV; Metagenome Assembled Genome; MAG; Third Party Data; TPA;
            TPA:assembly.
SOURCE      Micropruina sp. (wastewater metagenome)
  ORGANISM  Micropruina sp.
            Bacteria; Bacillati; Actinomycetota; Actinomycetes;
            Propionibacteriales; Nocardioidaceae; Micropruina.
REFERENCE   1  (bases 1 to 5940)
  AUTHORS   Abdulkadir,N., Saraiva,J.P., Zhang,J., Stolte,S., Gillor,O.,
            Harms,H. and Rocha,U.
  TITLE     Genome-centric analyses of 165 metagenomes show that mobile genetic
            elements are crucial for the transmission of antimicrobial
            resistance genes to pathogens in activated sludge and wastewater
  JOURNAL   Microbiol Spectr 12 (3), e0291823 (2024)
   PUBMED   38289113
REFERENCE   2  (bases 1 to 5940)
  AUTHORS   Abdulkadir,N.
  TITLE     Direct Submission
  JOURNAL   Submitted (24-MAR-2023) Environmental Microbiology, Helmholtz
            Center for Environmental Research -UFZ, Permoserstr 15, Leipzig
            04318, Germany
COMMENT     The annotation was added by the NCBI Prokaryotic Genome Annotation
            Pipeline (PGAP). Information about PGAP can be found here:
            https://www.ncbi.nlm.nih.gov/genome/annotation_prok/
            
            ##Genome-Assembly-Data-START##
            Assembly Method        :: SPAdes v. 3.11.1
            Genome Representation  :: Full
            Expected Final Version :: Yes
            Genome Coverage        :: Not Applicable
            Sequencing Technology  :: Illumina MiSeq
            ##Genome-Assembly-Data-END##
            
            ##Genome-Annotation-Data-START##
            Annotation Provider               :: NCBI
            Annotation Date                   :: 05/04/2023 04:57:10
            Annotation Pipeline               :: NCBI Prokaryotic Genome
                                                 Annotation Pipeline (PGAP)
            Annotation Method                 :: Best-placed reference protein
                                                 set; GeneMarkS-2+
            Annotation Software revision      :: 6.5
            Features Annotated                :: Gene; CDS; rRNA; tRNA; ncRNA
            Genes (total)                     :: 2,776
            CDSs (total)                      :: 2,750
            Genes (coding)                    :: 2,714
            CDSs (with protein)               :: 2,714
            Genes (RNA)                       :: 26
            tRNAs                             :: 24
            ncRNAs                            :: 2
            Pseudo Genes (total)              :: 36
            CDSs (without protein)            :: 36
            Pseudo Genes (ambiguous residues) :: 0 of 36
            Pseudo Genes (frameshifted)       :: 2 of 36
            Pseudo Genes (incomplete)         :: 32 of 36
            Pseudo Genes (internal stop)      :: 2 of 36
            ##Genome-Annotation-Data-END##
FEATURES             Location/Qualifiers
     source          1..5940
                     /organism="Micropruina sp."
                     /mol_type="genomic DNA"
                     /submitter_seqid="NODE_6211_length_5940_cov_4.159218"
                     /isolate="AMR_MDS_2195"
                     /isolation_source="Activated sludge of municipal
                     wastewater treatment plants"
                     /db_xref="taxon:2737536"
                     /environmental_sample
                     /geo_loc_name="Canada"
                     /lat_lon="49.25 N 123.1 W"
                     /collection_date="2015-06-16"
                     /metagenome_source="wastewater metagenome"
                     /note="metagenomic"
     gene            296..1717
                     /locus_tag="PLF56_05945"
     CDS             296..1717
                     /locus_tag="PLF56_05945"
                     /EC_number="2.7.7.72"
                     /inference="COORDINATES: protein motif:HMM:TIGR02692.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_function: GO:0003723 - RNA binding [Evidence IEA];
                     GO_function: GO:0016779 - nucleotidyltransferase activity
                     [Evidence IEA];
                     GO_process: GO:0006396 - RNA processing [Evidence IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="CCA tRNA nucleotidyltransferase"
                     /protein_id="HOQ53140.1"
                     /translation="MQELRTGSSAVRRLTSLFADAGHELYLVGGPVRDALLGRRVHDL
                     DFTTSARPDAIEALLRQLSPAVWDIGREFGTIGCAVTETGQDGAPAKLVIEVTTFRSD
                     AYDPGSRKPSVVFGEHLGGDLVRRDFTVNAMALDLASGELLDPFGGASDLVNGVLMTP
                     STPELSFSDDPLRMLRAARFVAQLDLVPAPEVVAAMTAMAERITIVSAERVREELIKT
                     IMAPRPRVGLNLLVATGLAGYVLPELPALRMERDEHNRHKDVYEHTLTVLDQTIDLEE
                     SRLGGPDLIARLAALLHDIGKPRTRRFEPGGKVSFHHHDVVGAKMATKRLQALKFPTE
                     QIKAVATLVELHLRFHGYADADWTDSAVRRYVRDAGDQLERLHVLTRSDCTTRNARKA
                     ARLRTAYEQLEWRIDELAEQEELSAMRPDLDGTQIMAILGIGPGPEVGRAYRFLLEKR
                     IDEGPLGEEAAEAALRAWWASQS"
     gene            1924..2535
                     /locus_tag="PLF56_05950"
     CDS             1924..2535
                     /locus_tag="PLF56_05950"
                     /inference="COORDINATES: ab initio
                     prediction:GeneMarkS-2+"
                     /note="Derived by automated computational analysis using
                     gene prediction method: GeneMarkS-2+."
                     /codon_start=1
                     /transl_table=11
                     /product="hypothetical protein"
                     /protein_id="HOQ53141.1"
                     /translation="MTDASSATTAVRPRRNLRWILIGVLAICLGGLGSAVLYSSVSNA
                     TTVLKVNRTIYRGEVIGAADLGPVSLGTHLGLETVPAERAPELVGKTALTDLPNGSLL
                     VPTAIGEAEVQRGYSRLGLRLTPGQVPVSPLPSGTAVVLVPIAAANDGEQSGASFKAT
                     VSVAPAMLADGNTSIEVTVADADSDQVARLAAAGRLVVVRRAG"
     gene            2535..3305
                     /locus_tag="PLF56_05955"
     CDS             2535..3305
                     /locus_tag="PLF56_05955"
                     /inference="COORDINATES: ab initio
                     prediction:GeneMarkS-2+"
                     /note="Derived by automated computational analysis using
                     gene prediction method: GeneMarkS-2+."
                     /codon_start=1
                     /transl_table=11
                     /product="hypothetical protein"
                     /protein_id="HOQ53142.1"
                     /translation="MAIVVLCSAGHAPGVTTTALGLALAWPRPVLLVDADRTPTQSVL
                     AGYLRGERPGHRGLGGLLQALRERRPFEGAIEAETVDLPPLLSGAQPRFLAGFPHPGV
                     VGLFAGAWPDFVSALAAQPGDVLIDAGRVGPEGLPLPLVREADLVLVVSRTTLVSLAA
                     LRLYLPALKDAADTDRTALALIGSSMPYSRAEVQDQFGTTVAADLPHDPDAAAVLSDG
                     LPAPRRYTGGAYFRGVTLAARSISGRLVAARELIGVRR"
     gene            3302..4774
                     /locus_tag="PLF56_05960"
     CDS             3302..4774
                     /locus_tag="PLF56_05960"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_016454918.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="CpaF/VirB11 family protein"
                     /protein_id="HOQ53143.1"
                     /translation="MTKLTTVPLLQDLPFETTVTADRLPAREPLDADAPDWRRTRPVK
                     PRDAASDEAGALDWSLVVVLRRKASELIAAAIADHHDRHGTALPEVDRRLLGRSLVKQ
                     VVRDHAEDLSSNGQALWPISLEHAYAEGVENAIFGYGRLQPLFEIPGAENIEIHGHDS
                     VMVQYGDGRREPRAQVADSDQELVEAIRFLGESVSPARPFDDAHPTMTLALGDRFRLH
                     AIGFGLSYRPSITIRQHILTDVSLKDLVGTGMMPDEVGRFLHAAVMARKSMVIAGDQG
                     AGKTTLLRALIEAIPGNERFGTLETDYELLTHLRPERRNMLALQAKVGMGEQQDGRRL
                     GEYSVADLIPEALRQNLSRLVIGEVRGTEAGAMFEAMQAGAGTLSTTHSHSAASTMDR
                     LASRVAQGGVLSVDEAYRQVAHHIHLLIYVRLVDDTWRGGRRTRRVAEIRQLTGALEA
                     GRPVTHLVYAAPTATTPAVFHPDPEFAAELAEFDDAWSLP"
     gene            4771..5616
                     /locus_tag="PLF56_05965"
     CDS             4771..5616
                     /locus_tag="PLF56_05965"
                     /inference="COORDINATES: protein motif:HMM:NF012694.2"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="type II secretion system F family protein"
                     /protein_id="HOQ53144.1"
                     /translation="MTALVALLGALVCTGVVLVVAGLRPSVRTQPVPHSSGLARRMRP
                     WLKRHGFRFAVGLIGGIAFAAFTGMGVMVLVVPVAVVGLPILLSAPSNREIDLLQALD
                     RWVRGLAAMLPTGRSITDAMRLSQRSAPELLVGPLALLVARLDDRWTSGEALRAMADE
                     LDSPDADAVLAALALAAERGGTGATATLHALADSIQDRLRAQREIETERAKPRLVVRQ
                     VTVITVAVLALVMVFGRDFFAPYGTPVGQTILLCLLGVYVMSLVVLRRMITPRRRERI
                     LRGAR"
     gene            5613..>5940
                     /locus_tag="PLF56_05970"
     CDS             5613..>5940
                     /locus_tag="PLF56_05970"
                     /inference="COORDINATES: ab initio
                     prediction:GeneMarkS-2+"
                     /note="Derived by automated computational analysis using
                     gene prediction method: GeneMarkS-2+."
                     /codon_start=1
                     /transl_table=11
                     /product="hypothetical protein"
                     /protein_id="HOQ53145.1"
                     /translation="MTGQWVAIASGVLLAAGLWALAGYFVPAHPRLGDALKLMDGRVR
                     APQPAATGADRIGAWARTRLPGGVDDRVARQLQLRAIGIDRFYTYKVIAAVTGLALPS
                     LIGVILL"
BASE COUNT          819 a         2076 c         2112 g          933 t
ORIGIN      
        1 ctctggctgt ctttcgagcc ggtggttcgc aacccggtga actcgacggt gagctggggc
       61 agggtggcct gcgcccgggc gggcggggcg ggcgcgacca gcaccacggt caccgtgacc
      121 aaccaggcca gcgcaagcgc gagcttggcc tgaagggtgc ggatcaccgt cccatcgtag
      181 ggtgccgccc ggtggcgtcc gggacgccgc gagcgtgccc ggccggtgag ccgcgagtgc
      241 gcgcccggta cagtgactcg acgtgcccga actgaccacc gcccagcagg ccgccttgca
      301 ggagttgcgc accggctcgt ccgcggtgcg ccggctgacc agcctgttcg cggacgcggg
      361 gcacgagctc tatctggtcg gcggccccgt gcgcgatgcc ctgctgggcc ggcgggtgca
      421 cgacctcgac ttcaccacct cggcccgtcc ggacgcgatc gaggcgttgc tgcggcagct
      481 gagccccgcg gtgtgggaca tcggacgcga gttcggcacg atcggctgcg ccgtcaccga
      541 gaccggccag gacggcgcgc cggccaagct ggtgatcgag gtgaccacct ttcgctccga
      601 cgcctacgac ccgggttcgc gcaagccgtc ggtggtgttc ggcgagcatc tgggcggcga
      661 cctggtgcgc cgggacttca cggtcaatgc gatggcgctc gatctggcct cgggcgaact
      721 gctcgacccg ttcgggggtg cgtccgacct ggtgaacggg gtgttgatga ccccctcgac
      781 gccggagttg tcgttcagcg acgatccgtt gcgcatgctg cgggcggccc ggttcgtggc
      841 ccaactcgac ctggtgccgg cgcccgaggt ggtggcggcg atgacggcga tggccgaacg
      901 gatcaccatc gtgtcggccg aacgggtgcg cgaagaactg atcaagacga tcatggcccc
      961 ccgcccacgg gtggggctga acctgttggt ggcgaccggc ctggccggct acgtgctgcc
     1021 cgaactgccg gcgttgcgca tggagcgcga cgagcacaac cggcacaagg acgtctacga
     1081 gcacaccctg acggtgctcg accagaccat cgatcttgaa gagtcgcggc tcggcggccc
     1141 cgacctgatc gctcggctcg cggccctgct gcacgacatc ggcaagcccc ggacgcggcg
     1201 gttcgagccg ggcggcaagg tgtcttttca tcatcacgac gtggtcggcg ccaagatggc
     1261 gaccaagcgc ctgcaggcac tgaagtttcc caccgagcag atcaaggcgg tggccacgct
     1321 ggtcgagttg cacctgcgct tccacggcta cgccgacgcc gactggaccg attctgcggt
     1381 gcggcgttac gtgcgcgacg cgggcgacca gctggagcgg ctgcacgtgc tgacccgctc
     1441 ggactgcacc acccgcaacg cgcgcaaggc ggcccggctg cgcacggcct acgagcagtt
     1501 ggagtggcgg atcgacgagc tcgccgaaca ggaggagctg agcgcgatgc gtccagacct
     1561 ggacggcacg cagatcatgg cgatcctggg catcgggccg ggccccgagg tgggccgggc
     1621 gtaccggttc ctgctcgaga agcgcatcga cgaggggccc ctgggagaag aggcggccga
     1681 ggccgcattg cgcgcctggt gggcgtctca gagctgacgt ttcacccagc gcccccctaa
     1741 ctttccaggg cccgcgaagc ttcgcgggcc agagatggtt agggaggcgc tcgggttttc
     1801 cgctgtcaaa tcgccacgcc gtgaattatc ggagttatcc acaaatttct tgagaccacc
     1861 cggaattgtc ggaaaccgca tttactctca acggacttgc agcccaccca gaggtactcc
     1921 gcgatgaccg acgcttcttc cgccaccacc gccgtcaggc cgaggcgcaa cctgcgctgg
     1981 atcctgatcg gcgtgctggc gatctgcctc ggcgggctcg gttctgcggt gctgtactcg
     2041 agcgtctcca acgccaccac ggtgctcaag gtgaatcgca cgatctatcg cggcgaggtg
     2101 atcggtgcgg ccgacctcgg tccggtcagc ctcggcaccc atctgggcct cgagacggtg
     2161 ccggccgagc gggcgcccga gctggtcggc aagacggcgc tgaccgacct gccgaacgga
     2221 tcgctcctgg tgccgaccgc gatcggcgag gccgaggtgc agcgcggcta ttcgcggctc
     2281 ggcctgcggt tgacgccggg ccaggtgccg gtctcaccgt tgccgtcggg caccgcggtg
     2341 gtgctggtgc cgatcgccgc cgccaacgac ggcgaacaat ccggcgcctc gttcaaagcc
     2401 accgtgtcgg tggcgccggc catgctcgcc gacggcaaca cctcgatcga ggtgaccgtc
     2461 gccgatgccg acagtgacca ggtggcccgg cttgcggcgg ccggacgcct ggtcgtggtc
     2521 aggcgggccg gctgatggcg atcgtggtgc tgtgttcggc cggtcacgcc cctggtgtca
     2581 ccaccaccgc gctgggcctg gcgctggcgt ggccccgtcc ggtgctgctg gtggacgccg
     2641 atcgcacccc gacccaatcg gtgctggccg gctacctgcg cggtgagcgt cccgggcacc
     2701 gtgggctggg cggtctgctg caggcgctgc gcgagcgccg tccgttcgag ggggccatcg
     2761 aggccgagac cgttgacctg ccgccactgt tgagcggcgc ccagccccgc ttcctggccg
     2821 gctttccgca tcctggcgtg gtgggcctgt tcgccggggc gtggcccgat ttcgtctctg
     2881 ccctggctgc gcagccgggc gacgtgctga tcgatgccgg acgagtcggc cccgaggggc
     2941 tgccgctgcc gttggttcgt gaggccgacc tggtgctggt ggtcagccgc accacgctgg
     3001 tgtcgctcgc cgcacttcgg ctgtacctgc cggcgctcaa ggacgccgcc gacaccgacc
     3061 gcaccgcgct cgcgctgatc ggcagttcga tgccgtactc gcgggccgag gtgcaggacc
     3121 agttcggcac caccgtcgcc gccgacctgc cgcacgaccc ggacgcggcc gcggtgctca
     3181 gcgacggtct gccggcgccg cgccggtaca ccggcggtgc ctactttcgc ggggtcacgc
     3241 tcgcggctcg ctcgatctcg ggtcgcctgg tcgcggcccg cgaactgatc ggagtgcggc
     3301 gatgaccaag ctgaccaccg tgccgctgct gcaggaccta cccttcgaaa ccaccgtcac
     3361 cgccgaccgg ttgccggccc gcgaacccct cgatgccgat gcgccggact ggcgccgcac
     3421 ccgtccggtc aagccgcggg acgccgcgtc cgacgaggcc ggcgcgctcg actggagcct
     3481 ggtggtggtg ctgcgccgca aggcgtccga actgatcgcc gccgcgatcg ccgaccacca
     3541 cgaccggcac ggcacggccc tgcccgaggt cgaccgccgg ctgctcggcc gctcgctggt
     3601 caagcaggta gtgcgtgacc atgccgaaga cctctcgagc aacggtcagg cactctggcc
     3661 gatctcgctc gagcacgcgt acgccgaggg ggtcgagaac gcgatcttcg gctacggccg
     3721 gctacagccg ctgttcgaga ttccgggcgc cgagaacatc gagatccacg ggcacgactc
     3781 ggtgatggtg cagtacggcg acggacgccg cgagcctcgc gcgcaggtgg ccgacagcga
     3841 tcaggagttg gtcgaggcga tcaggtttct gggcgaatcg gtcagcccgg cccgtccctt
     3901 cgacgacgcg caccccacca tgaccctggc cctgggcgat cggttccggc tgcatgcgat
     3961 cggtttcggc ttgtcgtacc ggccctcgat cacgattcgc caacacattc tcaccgacgt
     4021 ctcgctcaaa gacctggtcg gcaccggcat gatgcccgac gaggtgggcc gctttctgca
     4081 tgccgcggtg atggcgcgca agtcgatggt gatcgctggc gatcagggag ccggcaagac
     4141 cacgctgttg cgcgcactga tcgaggcgat accgggcaat gaacgcttcg gcacgctcga
     4201 gaccgactac gaactgctca cccacctgcg ccccgagcgg cgcaacatgc tggccctgca
     4261 ggcgaaggtg ggcatgggtg agcagcagga cgggcggcgg ctgggcgaat actccgtcgc
     4321 cgacctgatt ccggaggcgc tgcggcagaa cctctcgcga ttggtcatcg gcgaggtgcg
     4381 cggcaccgag gcgggggcga tgttcgaggc catgcaggcc ggcgcgggca ccctgagcac
     4441 gacccactcg cactcggcgg cctcgaccat ggatcggctc gcctcgcggg tggcgcaggg
     4501 tggcgtcctg agtgtcgatg aggcctatcg ccaggtcgcc caccacattc acctgctcat
     4561 ctacgtgcgc ctggtggacg acacctggcg tggcggacgc cgcacccggc gggtggccga
     4621 gatccgtcaa ctcaccggtg cgctcgaagc cggacggccg gtcacccacc tggtgtacgc
     4681 cgcgccgacc gccaccacac cggccgtctt ccaccccgac cccgagttcg cggccgagct
     4741 ggccgagttc gacgacgcct ggagcctgcc gtgacggccc tcgttgcgct cctgggcgcg
     4801 ctggtctgca ccggcgtggt gctggtggtg gcgggcctgc ggccgagcgt ccggacccag
     4861 ccggtgcctc acagttccgg gctggcgcgg cgaatgcgtc cctggctgaa gagacacggc
     4921 ttccggttcg cggtcggttt gatcggcggc atcgcgttcg cggccttcac cggcatgggc
     4981 gtgatggtgc tggtggtgcc ggtcgccgtg gtgggtctgc cgatcctgct gagcgccccg
     5041 tccaaccgcg agatcgacct gttgcaggca ctcgaccgct gggttcgggg cctggcggcc
     5101 atgttgccca ccggacgttc gatcaccgac gcgatgcgac tgtcgcagcg ctccgccccc
     5161 gaactgctgg tcggcccact cgcactgctg gtggcccggc tggacgatcg ctggacctcg
     5221 ggtgaggcgc tgcgagcgat ggccgacgag ctggactcgc ccgacgccga cgcggtgctg
     5281 gccgccctgg cgttggcggc cgagcgcggc ggcaccggcg ccacggccac gctgcatgcc
     5341 ctcgccgatt cgatccagga ccggctccgg gcccagcgcg agatcgagac cgagcgggcc
     5401 aagccgcggc tggtggtgcg ccaggtcacc gtgatcacgg tggcggtgct ggctctggtg
     5461 atggtgttcg gccgagactt cttcgccccc tacggcacgc cggtcggcca aacgattctg
     5521 ctctgcctgc tgggcgtgta cgtgatgtcg ttggtggtgc tgcgccgcat gatcaccccc
     5581 cggcgccggg aacgcattct gcgcggtgca cgatgaccgg ccagtgggtg gcgatcgcgt
     5641 cgggcgtgct gctcgccgcc gggttgtggg cgttggccgg ctacttcgtg cccgcccatc
     5701 cgcgcctggg cgacgcgctc aagctgatgg acggacgcgt ccgcgccccg cagcccgccg
     5761 ccaccggcgc cgaccggatc ggggcctggg cccgcacccg gctacccggc ggtgtggacg
     5821 accgcgtcgc acgacagctg caactgcggg ccatcggaat cgatcgcttc tacacctaca
     5881 aggtgatcgc cgcggtgacc ggcctcgcgc tgcccagcct gatcggtgtg atcctgctgc
//
LOCUS       DAOSUG010000140         5917 bp    DNA     linear   ENV 10-JUN-2024
DEFINITION  MAG TPA_asm: Micropruina sp. isolate AMR_MDS_2195
            NODE_6233_length_5917_cov_4.804674, whole genome shotgun sequence.
ACCESSION   DAOSUG010000140 DAOSUG010000000
VERSION     DAOSUG010000140.1
DBLINK      BioProject: PRJNA925477
            BioSample: SAMN32864262
            Sequence Read Archive: SRR4236673
KEYWORDS    WGS; ENV; Metagenome Assembled Genome; MAG; Third Party Data; TPA;
            TPA:assembly.
SOURCE      Micropruina sp. (wastewater metagenome)
  ORGANISM  Micropruina sp.
            Bacteria; Bacillati; Actinomycetota; Actinomycetes;
            Propionibacteriales; Nocardioidaceae; Micropruina.
REFERENCE   1  (bases 1 to 5917)
  AUTHORS   Abdulkadir,N., Saraiva,J.P., Zhang,J., Stolte,S., Gillor,O.,
            Harms,H. and Rocha,U.
  TITLE     Genome-centric analyses of 165 metagenomes show that mobile genetic
            elements are crucial for the transmission of antimicrobial
            resistance genes to pathogens in activated sludge and wastewater
  JOURNAL   Microbiol Spectr 12 (3), e0291823 (2024)
   PUBMED   38289113
REFERENCE   2  (bases 1 to 5917)
  AUTHORS   Abdulkadir,N.
  TITLE     Direct Submission
  JOURNAL   Submitted (24-MAR-2023) Environmental Microbiology, Helmholtz
            Center for Environmental Research -UFZ, Permoserstr 15, Leipzig
            04318, Germany
COMMENT     The annotation was added by the NCBI Prokaryotic Genome Annotation
            Pipeline (PGAP). Information about PGAP can be found here:
            https://www.ncbi.nlm.nih.gov/genome/annotation_prok/
            
            ##Genome-Assembly-Data-START##
            Assembly Method        :: SPAdes v. 3.11.1
            Genome Representation  :: Full
            Expected Final Version :: Yes
            Genome Coverage        :: Not Applicable
            Sequencing Technology  :: Illumina MiSeq
            ##Genome-Assembly-Data-END##
            
            ##Genome-Annotation-Data-START##
            Annotation Provider               :: NCBI
            Annotation Date                   :: 05/04/2023 04:57:10
            Annotation Pipeline               :: NCBI Prokaryotic Genome
                                                 Annotation Pipeline (PGAP)
            Annotation Method                 :: Best-placed reference protein
                                                 set; GeneMarkS-2+
            Annotation Software revision      :: 6.5
            Features Annotated                :: Gene; CDS; rRNA; tRNA; ncRNA
            Genes (total)                     :: 2,776
            CDSs (total)                      :: 2,750
            Genes (coding)                    :: 2,714
            CDSs (with protein)               :: 2,714
            Genes (RNA)                       :: 26
            tRNAs                             :: 24
            ncRNAs                            :: 2
            Pseudo Genes (total)              :: 36
            CDSs (without protein)            :: 36
            Pseudo Genes (ambiguous residues) :: 0 of 36
            Pseudo Genes (frameshifted)       :: 2 of 36
            Pseudo Genes (incomplete)         :: 32 of 36
            Pseudo Genes (internal stop)      :: 2 of 36
            ##Genome-Annotation-Data-END##
FEATURES             Location/Qualifiers
     source          1..5917
                     /organism="Micropruina sp."
                     /mol_type="genomic DNA"
                     /submitter_seqid="NODE_6233_length_5917_cov_4.804674"
                     /isolate="AMR_MDS_2195"
                     /isolation_source="Activated sludge of municipal
                     wastewater treatment plants"
                     /db_xref="taxon:2737536"
                     /environmental_sample
                     /geo_loc_name="Canada"
                     /lat_lon="49.25 N 123.1 W"
                     /collection_date="2015-06-16"
                     /metagenome_source="wastewater metagenome"
                     /note="metagenomic"
     gene            complement(177..725)
                     /locus_tag="PLF56_05975"
     CDS             complement(177..725)
                     /locus_tag="PLF56_05975"
                     /inference="COORDINATES: protein motif:HMM:NF024698.2"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_function: GO:0008080 - N-acetyltransferase activity
                     [Evidence IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="GNAT family N-acetyltransferase"
                     /protein_id="HOQ53146.1"
                     /translation="MPTLTTGRLTLRDWRADDADFVFDLYSRPEVQRYIGRPPTVMRS
                     RDEADARITRWTGLHHPVHHIWAVERTADHRLLGTLLLKDIPRTGTDGAPSSETEIGW
                     HLHPDAWGHGYAAEAGRRVLEYALAEGIDRVIAVTNPVNRASQGVCRRIGMTPLGLTE
                     DYYDLTCALFEARTPLPPFAPH"
     gene            946..1671
                     /locus_tag="PLF56_05980"
     CDS             946..1671
                     /locus_tag="PLF56_05980"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_020575742.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="DNA alkylation repair protein"
                     /protein_id="HOQ53147.1"
                     /translation="MNPVTHLADAVRTALAQAADPNRAVGQQRYMRSTLPFYGVSVTQ
                     TRSIVRAAVKEHPPSDRASWLAIVRALWDGATHREERYAAIAVLRQRGWRRWLYADDE
                     LLALLRHLIVTGAWWDLVDDLASHVAGDLLRAEPGTMRPLLLGWARADDLWLRRTAIL
                     SQLLSRASTDPDLLAYAIEGSLDDPDFFARKAIGWALREYSKTDERWVRTFVESNVDR
                     LSPLSVREALSRAERPPNHRAPA"
     gene            complement(1724..2410)
                     /locus_tag="PLF56_05985"
     CDS             complement(1724..2410)
                     /locus_tag="PLF56_05985"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_020390658.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_function: GO:0003677 - DNA binding [Evidence IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="helix-turn-helix domain-containing protein"
                     /protein_id="HOQ53148.1"
                     /translation="MTSTREAQRGARRHQSTASGPSARRQATRHRLMDAAVELFAERG
                     VLAASVEEICERAGFTRGAFYSNFESKDELCLGVLRRKCEQHVSAMQAAIDMISEVSA
                     DPDGRPNTRQLIREAVRVFLEAQPKEMPEQIAMMELRLHAVRTPELRAGWLAVHEGIT
                     SAVTDLLDIALRRMGARLAMPNAAVIELLGAVYENTVVMSLLNGERRPTGLADQLTTL
                     LEALVVRDCE"
     gene            2503..5157
                     /locus_tag="PLF56_05990"
     CDS             2503..5157
                     /locus_tag="PLF56_05990"
                     /inference="COORDINATES: protein motif:HMM:NF015155.2"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_component: GO:0016020 - membrane [Evidence IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="MMPL family transporter"
                     /protein_id="HOQ53149.1"
                     /translation="MSSMLYRIGRACYRHGVRVLAVWLVVLGALGGAVALAGGAQFND
                     SFKIPGASSQVALDQLKMTFPSAAAATAQVVVLLPEGQSLNDKDVRPKVEKALDTFKS
                     LPIVDDVVTPYSEHVKGLISDNGRAGLASVQLNVTMTSITDAQKQSLEAQAEKVAAQL
                     PGSQVHLGGEVFSTHIPHISIIEGLGVVVAIVVLLVVLGSFTAAAMPVGTALIGVGIA
                     VEITMIASRVIEVNSTSLMLAVMISLAVGIDYALFIVSRHRDQLSEGLDAEESTARAV
                     ATAGSAVVFAGLTVIIALVGLSISGIPFLGVMGVFSAVGVGVEVLLALTLLPAFLGFA
                     GERVRPKAAKPTKSGRAPSPRFDLRRVWVRLVTAVPLLTVALVIAALGLLALPARDLQ
                     LALPNSGRNPASYGDRITFDVISREFGIGYNGPLVVTGTIVESDDPLKITDGLKKQIE
                     AMPGVQLVPLATPNENADTALIQVIPTTGPDDPATAELVQRLRDQHQHWLDEYGIDTS
                     VTGATAVQIDVSQRLSDALIPFAIFVVGLSLVLLTLVFRSLWVPLKAAVGFLLSVAAA
                     FGATALVFNQGWFKQLVNLGEPGPVISFLPIILMGVLFGLAMDYEVFLVSRMREEFVH
                     GNRDTFVEEGFVHSSRVVVAAALIMFAVFAFFVPSGEGAIKPIAFALAIGVVLDAFVV
                     RMTLVPAVMKLLGTHAWWLPRWLDRHLPMVDIEGETLAHQLKLQSWPAAGGHVIYGRD
                     LACGEAFSGVDVAVKPGEVLVVEGETSARRALLMALAGRLAFTAGDIVVAGRVLPEQS
                     SEIRRLAAVATTGHPSPIHNFNGPVLMVDEADQFTRAERAALAERLRTLTEAGGCAVL
                     AHAPGADLSDVVPAAYRTLHLQGELSHA"
     gene            5150..>5917
                     /locus_tag="PLF56_05995"
     CDS             5150..>5917
                     /locus_tag="PLF56_05995"
                     /inference="COORDINATES: protein motif:HMM:TIGR03061.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="YhgE/Pip family protein"
                     /protein_id="HOQ53150.1"
                     /translation="MPEATRSFSWRTLLALVMIPVLVAGGFLWGTWNSGDRLHQVEAA
                     VVNLDEGVTLNDQFVPLGRQLSADLVDSSKAQNFTWVLADEAHAWPGLASGRYAAVVV
                     IPKNFSKAATSYGNDDPLTAEQATIQVHTSPVAGVADAWLGNVLATQASSTLNATLTK
                     AYLDQIYIGFNDTGKQFQTVADAAGKLADGTDELSDGIVKASDGAGQLSTGMGKLSDG
                     SPALVTGVRQVETGAGALATGLQQMKKQTADLPDQGKE"
BASE COUNT          873 a         1960 c         2056 g         1028 t
ORIGIN      
        1 ggcccggtcg gctctgctgc cgctgcggct catacgcggc ggatgcgccg gcttcgttca
       61 acgcctggtg attcaacgca tggtgacata ccgaatctac cgctcgattc gcaagtttcc
      121 gggattgaat cgttcaaacc agaatcgagc ggtggatttg gtatccgtgc cgttggtcag
      181 tgtggcgcga acggcggcag cggggtccgc gcctcgaaga gcgcgcaggt caggtcgtag
      241 tagtcctcgg tgagtccgag cggggtcatg ccgatgcggc gacacacccc ttgcgaggct
      301 ctgttgacgg gattcgtgac cgcgatcacc cggtcgatgc cttcggcgag cgcgtactcc
      361 agcactcgcc gtcccgcctc ggcggcgtag ccgtgacccc aggcgtcggg gtgcaggtgc
      421 cagccgatct cggtctcgct cgagggcgcc ccgtcggtcc cggtgcgcgg aatgtccttc
      481 agcagcaacg tgcccagcag gcggtggtcg gccgtgcgtt cgacggccca gatgtgatgg
      541 acggggtggt gcaggccagt ccagcgggtg atccgggcgt ccgcctcgtc gcggcttcgc
      601 atcacggtcg gtgggcgacc gatgtagcgc tgcacctccg gccgcgaata caggtcgaac
      661 acgaagtcgg catcatcggc ccgccagtcg cgcagggtca gccggccggt ggtgagggtt
      721 ggcatggctc catgatcact gatcatgggg acgacgacct cagcgtggac ccccgcggcg
      781 gtgtgcctca gtacttggtc tcgacgcgac ccacgagccg agtgacgaag cggcctcggc
      841 tcgtggctgc agtctgcgtc gtctgcttcg tctgcttcgt ctgcttgggc gactcggcta
      901 ccgatgcggc cgggtacggt ccagtcggtg tgctgcaatg gcgccgtgaa ccctgtcacg
      961 cacctggccg acgccgtgcg caccgccctg gcgcaggctg ccgaccccaa ccgcgccgtc
     1021 ggtcagcagc gctacatgcg ctccactctg ccgttctacg gcgtgagcgt gacgcagact
     1081 cgcagcatcg tccgggcggc ggtgaaggag cacccgccgt ccgaccgcgc gagctggctg
     1141 gcgatcgtcc gggcgctgtg ggacggggcc acccaccgtg aggaacgcta tgccgcgatc
     1201 gccgtgctgc ggcagcgcgg gtggcgacgt tggctgtacg ccgacgacga gttgctcgca
     1261 ctattgcgac atctgatcgt gaccggcgcc tggtgggatc tggtggacga cctcgcctca
     1321 catgtggcgg gggatctgct gcgggccgag ccgggcacga tgaggccgct gctgctgggg
     1381 tgggcgcgcg cggacgacct gtggttgcgt cgcacggcga tcctgagcca gctgctctcc
     1441 cgcgccagca cggaccccga cctgctcgcg tacgcgatcg agggcagcct ggacgacccg
     1501 gacttcttcg cccgcaaggc catcggctgg gctttgcgcg agtactcgaa gacggacgag
     1561 cgctgggtgc gcaccttcgt cgagtcaaac gtggaccggt tgtcaccgct gtcagtccgc
     1621 gaggcgctgt cgcgcgccga gcgcccccct aaccatcgtg cgcccgcgta gcttcgcggg
     1681 ccctggaaag ttaggggggc gctcggcgaa gtccggacgt tccctactcg cagtcgcgaa
     1741 ccaccagggc ctcgagcagc gtggtgagtt gatcggccag cccggtgggc cgccgctcgc
     1801 cgttcagcag gctcatcacc acggtgttct catacaccgc gccgagcagc tcgatcactg
     1861 ccgcattggg catcgccagc cgggcaccca tgcgccgcag cgcgatatcg agcagatccg
     1921 tgaccgctga ggtgatgcct tcgtgcaccg ccagccaccc ggcccgcagc tcgggggtgc
     1981 gcacggcgtg cagccgcagc tccatcatgg cgatctgctc gggcatctct ttcggttgtg
     2041 cctcgagaaa cacccgcacg gcctcgcgga tcagttgtcg ggtgttcggc cgcccgtcag
     2101 ggtctgcgct gacttcggag atcatgtcga tggccgcctg catcgcgctg acgtgctgct
     2161 cgcacttgcg gcgaagcacg cccaggcaca gctcgtcctt cgactcgaag ttcgagtaga
     2221 acgccccccg ggtgaatccg gcccgctcac agatctcttc caccgaggcg gccaggacgc
     2281 cccgttcggc gaacagttcg accgcggcgt ccatcaggcg gtggcgggtg gcttggcggc
     2341 gtgcactcgg cccggacgcc gtcgactgat gccgacgagc tccgcgctga gcctctcggg
     2401 tgctggtcat ctcgttgatc ctaccggttt gcatacacac ttgtactgaa tacactactg
     2461 cattggatac agtcgtgtat tgagatcgag agggggcggc cgatgtcgtc gatgctgtat
     2521 cggatcggac gggcctgcta ccggcacggg gtccgcgtgc tggccgtctg gctcgtcgtc
     2581 ctgggcgcac tcggcggagc ggtggccctg gccggcggcg cccagttcaa cgacagcttc
     2641 aagattccgg gtgcctcgtc gcaggtcgca ctcgaccagc tgaagatgac gttcccctcc
     2701 gcggccgccg ccaccgccca ggtggtggtg ctgctgcccg agggtcaatc cctcaacgac
     2761 aaggatgttc gccccaaggt tgaaaaggct cttgacacct tcaagtcgct gccgatcgtc
     2821 gacgacgtgg tcaccccgta cagcgagcac gtcaagggcc tgatcagcga caacggacgc
     2881 gccggcctgg ccagcgtgca gctcaacgtg acgatgacgt ccatcaccga tgcgcagaag
     2941 cagagcctgg aggcccaggc cgagaaggtc gcagcacaac tgcccggctc gcaggtgcat
     3001 ctgggcggcg aggtgttcag cacgcacatt ccgcacatca gcatcatcga gggcctcggt
     3061 gtcgtggtcg ccatcgtggt gctgctggtc gtgctcgggt cgttcaccgc ggcggcaatg
     3121 ccggtcggca ccgcgctgat cggggtgggc atcgcggtcg agatcaccat gatcgcctcg
     3181 cgtgtgatcg aggtgaacag caccagcctg atgctggcgg tgatgatttc gctggcggtg
     3241 ggcatcgact acgcgctgtt catcgtcagc cgtcaccgcg atcagctcag cgaaggcctt
     3301 gatgcggagg agtcgacggc ccgagccgtc gccaccgcgg gttcggcggt cgtcttcgcc
     3361 ggcctgaccg tgatcatcgc cctggtcggc ctgtcgatct ctggcattcc gttcctgggc
     3421 gtgatgggcg tgttctcggc cgtcggcgtc ggcgtcgagg tgctgctggc cctgaccctg
     3481 ctgcccgcct ttctcggctt tgcgggcgaa cgagtgcgcc ccaaggccgc gaaaccgacc
     3541 aaatccggac gcgccccgtc cccccgcttc gacctgcgcc gggtgtgggt gcgcctggtc
     3601 accgccgtgc cgctgctcac cgttgcgctg gtgatcgcgg cgctcggcct gctggcgctg
     3661 ccggcccgcg acctgcagct ggcgctgccc aactccggac gcaacccggc cagctacggc
     3721 gaccggatca cgttcgacgt gatcagccgc gagttcggca tcggctacaa cgggcctctg
     3781 gtggtgaccg gcacgatcgt cgaatcggat gatccgttga agatcaccga cgggctgaag
     3841 aagcagatcg aggccatgcc cggcgtgcag ctggtgccgc tggccacgcc gaacgagaac
     3901 gccgacaccg cgctgatcca ggtgattccc accaccgggc cggacgatcc ggccacggcc
     3961 gagctcgtcc aacggctgcg ggatcaacat cagcactggc tcgacgagta cggcatcgac
     4021 acctcggtga cgggggcgac ggccgtccag atcgacgtca gccagcgact gtcggacgca
     4081 ctgatcccgt tcgcgatctt cgtggtcggg ttgtcgctgg tgctgctgac cctggtgttc
     4141 cgctcgctgt gggtgccgct gaaggccgcg gtcgggtttc tgctgagcgt ggcggcggcc
     4201 ttcggggcca ccgcgctggt gttcaaccag ggctggttca agcaactggt caacctgggc
     4261 gaacccgggc cggtgatcag ctttctgccg atcattctga tgggcgtgct gttcggcctc
     4321 gccatggatt acgaggtgtt cctggtcagc cgcatgcgcg aggagttcgt gcacggcaac
     4381 cgcgacacct tcgtcgagga gggcttcgtg cattcctcgc gggtagtcgt ggccgcggcc
     4441 ctgatcatgt tcgccgtgtt cgccttcttc gtgccctcgg gcgagggtgc gatcaagccg
     4501 atcgcgttcg ccctggccat cggcgtggtg ctggatgcgt tcgtggtgcg catgacgctg
     4561 gtaccggcgg tgatgaagct gctcggcacg catgcctggt ggctgccccg ctggctcgac
     4621 cggcacctgc cgatggtcga catcgagggc gagacgctgg cccaccagct caagctgcag
     4681 agctggccgg cggccggcgg gcacgtgatc tacggtcgcg acctggcctg cggggaggcc
     4741 ttctcgggtg tcgacgtcgc ggtcaagccg ggggaggtgc tggtcgtcga gggcgaaacc
     4801 tcggcccggc gggcgctgct gatggccctg gccggacggc tggcgttcac cgccggcgac
     4861 atcgtggtgg ccggtcgggt gctgcccgag cagagctccg agatccgacg gctggcggcg
     4921 gtggcgacca cggggcaccc gtccccgatt cacaacttca acggtccggt gctgatggtc
     4981 gacgaggccg accagttcac ccgcgccgaa cgtgcggcgc tcgccgagcg actgcgcacg
     5041 ctgaccgagg cgggcggctg tgccgtgttg gcccacgccc ccggcgcgga cctttccgat
     5101 gtggtgcccg ccgcgtaccg caccctgcat cttcaaggag aactcagtca tgcctgaagc
     5161 cacacgttcg ttctcgtggc gcaccctgct cgccctggtg atgattccgg tgctggtcgc
     5221 cggcggcttt ctgtggggca cctggaactc gggggaccgg ctgcaccagg tcgaggccgc
     5281 cgtggtcaac ctcgacgagg gggtcacgtt gaacgaccag ttcgttccgc tcggacggca
     5341 gttgtcggcc gacctggtcg actccagcaa ggcgcagaac ttcacctggg tgctggccga
     5401 tgaggcacac gcctggccgg gtctggcgtc cggacgctac gccgcggtcg tggtgattcc
     5461 gaagaacttc tccaaggcgg ccacgtcgta cggcaacgac gacccactga ccgccgagca
     5521 ggcgacgatt caggtgcaca cctcgccggt ggcaggggtc gcggatgcgt ggctgggcaa
     5581 tgtgctggcc acgcaggcgt ccagcacgct gaacgcgacg ctgaccaagg cctacctcga
     5641 ccagatctac atcggcttca acgacaccgg caagcagttc cagacggttg cggacgccgc
     5701 aggcaagctc gccgacggca cggacgagct cagtgacggc attgtcaagg catcggacgg
     5761 ggccgggcag ctgagcacgg gcatgggcaa gctgtcggac gggtcgcccg cgctggtgac
     5821 tggcgtgcga caggtggaaa ccggggccgg cgcgctggcc acggggctgc agcagatgaa
     5881 gaagcagacc gcggacctgc ccgatcaggg caaggaa
//
LOCUS       DAOSUG010000141         5908 bp    DNA     linear   ENV 10-JUN-2024
DEFINITION  MAG TPA_asm: Micropruina sp. isolate AMR_MDS_2195
            NODE_6240_length_5908_cov_4.612848, whole genome shotgun sequence.
ACCESSION   DAOSUG010000141 DAOSUG010000000
VERSION     DAOSUG010000141.1
DBLINK      BioProject: PRJNA925477
            BioSample: SAMN32864262
            Sequence Read Archive: SRR4236673
KEYWORDS    WGS; ENV; Metagenome Assembled Genome; MAG; Third Party Data; TPA;
            TPA:assembly.
SOURCE      Micropruina sp. (wastewater metagenome)
  ORGANISM  Micropruina sp.
            Bacteria; Bacillati; Actinomycetota; Actinomycetes;
            Propionibacteriales; Nocardioidaceae; Micropruina.
REFERENCE   1  (bases 1 to 5908)
  AUTHORS   Abdulkadir,N., Saraiva,J.P., Zhang,J., Stolte,S., Gillor,O.,
            Harms,H. and Rocha,U.
  TITLE     Genome-centric analyses of 165 metagenomes show that mobile genetic
            elements are crucial for the transmission of antimicrobial
            resistance genes to pathogens in activated sludge and wastewater
  JOURNAL   Microbiol Spectr 12 (3), e0291823 (2024)
   PUBMED   38289113
REFERENCE   2  (bases 1 to 5908)
  AUTHORS   Abdulkadir,N.
  TITLE     Direct Submission
  JOURNAL   Submitted (24-MAR-2023) Environmental Microbiology, Helmholtz
            Center for Environmental Research -UFZ, Permoserstr 15, Leipzig
            04318, Germany
COMMENT     The annotation was added by the NCBI Prokaryotic Genome Annotation
            Pipeline (PGAP). Information about PGAP can be found here:
            https://www.ncbi.nlm.nih.gov/genome/annotation_prok/
            
            ##Genome-Assembly-Data-START##
            Assembly Method        :: SPAdes v. 3.11.1
            Genome Representation  :: Full
            Expected Final Version :: Yes
            Genome Coverage        :: Not Applicable
            Sequencing Technology  :: Illumina MiSeq
            ##Genome-Assembly-Data-END##
            
            ##Genome-Annotation-Data-START##
            Annotation Provider               :: NCBI
            Annotation Date                   :: 05/04/2023 04:57:10
            Annotation Pipeline               :: NCBI Prokaryotic Genome
                                                 Annotation Pipeline (PGAP)
            Annotation Method                 :: Best-placed reference protein
                                                 set; GeneMarkS-2+
            Annotation Software revision      :: 6.5
            Features Annotated                :: Gene; CDS; rRNA; tRNA; ncRNA
            Genes (total)                     :: 2,776
            CDSs (total)                      :: 2,750
            Genes (coding)                    :: 2,714
            CDSs (with protein)               :: 2,714
            Genes (RNA)                       :: 26
            tRNAs                             :: 24
            ncRNAs                            :: 2
            Pseudo Genes (total)              :: 36
            CDSs (without protein)            :: 36
            Pseudo Genes (ambiguous residues) :: 0 of 36
            Pseudo Genes (frameshifted)       :: 2 of 36
            Pseudo Genes (incomplete)         :: 32 of 36
            Pseudo Genes (internal stop)      :: 2 of 36
            ##Genome-Annotation-Data-END##
FEATURES             Location/Qualifiers
     source          1..5908
                     /organism="Micropruina sp."
                     /mol_type="genomic DNA"
                     /submitter_seqid="NODE_6240_length_5908_cov_4.612848"
                     /isolate="AMR_MDS_2195"
                     /isolation_source="Activated sludge of municipal
                     wastewater treatment plants"
                     /db_xref="taxon:2737536"
                     /environmental_sample
                     /geo_loc_name="Canada"
                     /lat_lon="49.25 N 123.1 W"
                     /collection_date="2015-06-16"
                     /metagenome_source="wastewater metagenome"
                     /note="metagenomic"
     gene            85..768
                     /locus_tag="PLF56_06000"
     CDS             85..768
                     /locus_tag="PLF56_06000"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_007077169.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="DUF305 domain-containing protein"
                     /protein_id="HOQ53151.1"
                     /translation="MTTKTRLGVAAIAASLAFVLAACSGGSQSGTATSDTTTARSTGS
                     PASISAAATATFNAADVAFAQMMIVHHQGAIQMADLAPSRAQNQDVLALAARIKAAQA
                     PEIDQMTSWLTAWGAAPSMMSGSTTSGTGGMDHGGMGGATSAAGSSMSMPGMMSGDQM
                     QQLESASGAAFDKMFLELMIVHHQGAIEMAETEIADGSNPEAVALAQKIKSDQTAEIA
                     EIQALLQTL"
     gene            870..2147
                     /locus_tag="PLF56_06005"
     CDS             870..2147
                     /locus_tag="PLF56_06005"
                     /inference="COORDINATES: protein motif:HMM:NF037017.2"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="Ig-like domain-containing protein"
                     /protein_id="HOQ53152.1"
                     /translation="MIASWVTRRPARTLGLVVVGALLLAACSAGEESVVVTQTVTEGA
                     GRPITAAPLSGSSSEPPESSQASRARFSASPRFGSTDVAPTQKVTVTAFNATITQVNA
                     VSDTDGTVLAGAISEDGGTWTLTDRMAYATSYRITGTASTRDGSTVPIDGQLSTVDPG
                     DTMRASFQLAEGGTYGVATPIILTFAGQVTDKAAAEAALSVMTDKGVVEGSWGWLQDE
                     DIQGTGVKQSRVHFRTKDYWPANTKVTVAANLYGIDLGGAWGREDLTRTFTIGRSQIV
                     TADVNSYRLVVTVDGAVTKNYPVSYGRATDNPDLETRSGVHVVQEKYDSFTMCNPKYG
                     YCGFVANWAVRISNNGEFIHENAAVVASLGKENVSHGCVNMSPADAKDYYDSALYGDP
                     VEVTGTTPQLGPSDGDIFDWTYPYDTWKTFSAT"
     gene            2258..3091
                     /locus_tag="PLF56_06010"
     CDS             2258..3091
                     /locus_tag="PLF56_06010"
                     /inference="COORDINATES: ab initio
                     prediction:GeneMarkS-2+"
                     /note="Derived by automated computational analysis using
                     gene prediction method: GeneMarkS-2+."
                     /codon_start=1
                     /transl_table=11
                     /product="hypothetical protein"
                     /protein_id="HOQ53153.1"
                     /translation="MTAATILLLAGCSAPVASTPASPAATAQGASSGLPSPHVHGIAI
                     DPADDTVYLATHDGLFSYGTGTPTRVGPIIDLMGFAAAGPTTFYASGHPGPGTALPNP
                     VGLLTSADAGRTWQPLSRQGESDFHALTAAPFGVIAADQALRITEDGRSWRELLIPQP
                     AIALAAAPDGGTILAATDAGPLRSTTRGDTWAPVDGAPPLQLLAWADADTAVGTTYDG
                     AVHISDDAGRSWSPRARVGPAQAITARAGADGNVRVLIVTRSEILESADGGVTFHPWV
                     P"
     gene            3206..3814
                     /locus_tag="PLF56_06015"
     CDS             3206..3814
                     /locus_tag="PLF56_06015"
                     /inference="COORDINATES: protein motif:HMM:NF012787.2"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_function: GO:0016491 - oxidoreductase activity
                     [Evidence IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="TlpA disulfide reductase family protein"
                     /protein_id="HOQ53154.1"
                     /translation="MLLRPPAGRRTRLRRWIAALGLALPLVLTGCGAGGGNNAFTFVS
                     PGGQNAFSYPSGQRQTVGDLSGPSLMDDGDRIVGMSDYADRVVVLNVWGSWCAPCRAE
                     APDLTSAARDLAPLGVQFIGIDVRDTRAGGRDFHTSFDVPYPSIFDPSMRTLLSLRGF
                     PTSSIPSTIVLDREHRVAQIWLRRITATELTATVRAIATEAA"
     gene            3816..4634
                     /locus_tag="PLF56_06020"
     CDS             3816..4634
                     /locus_tag="PLF56_06020"
                     /inference="COORDINATES: protein motif:HMM:NF014713.2"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="cytochrome c biogenesis CcdA family protein"
                     /protein_id="HOQ53155.1"
                     /translation="MASLATAATLVTAPEQTITDVVVSGPLTLAIMLAFAAGTVSFAS
                     PCCIPLVPGYLSYLAGLVGADTTRAAGEGDLRVRRRAVGATLLFIAGFTAVFLAQTAA
                     VLGVAHALLSDGELLMRVGGTVTIVMGLVMLGFIQPLQREFRIHARPTGPVAGALLLG
                     GTFGLGWVVCIGPTLAGIIALATATDWGGSAWRGMLLVVFYCLGLGLPFLLLSIGLGW
                     AATTVGFLRRHSRTLQVIGGASMVILGVLMLTGLWGQFVAWLQVALSGSTRVWL"
     gene            4694..>5908
                     /locus_tag="PLF56_06025"
     CDS             4694..>5908
                     /locus_tag="PLF56_06025"
                     /inference="COORDINATES: protein motif:HMM:NF016995.2"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_component: GO:0016020 - membrane [Evidence IEA];
                     GO_process: GO:0017004 - cytochrome complex assembly
                     [Evidence IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="cytochrome c biogenesis protein ResB"
                     /protein_id="HOQ53156.1"
                     /translation="MLPRRWRRILAPVRTLWRRLTSMRTALVLLFLLAVAALPGALLP
                     QWNLNRAKTTQYLLDHPVLGPWLDRLGFLAVFSAPWFAAIYLLLFTSLVGCLVPRTLD
                     LLRRLRRPPPRTPRHLSRLPHHAIREVAGPPEVVAIRIGRGLRRWRVRRTVEDTGAVS
                     IAAEKGYLRELGNLLFHLSLLGLLVAMAVGKLVGYEGSVIVDVGNGFCSVGPTVYDDF
                     RPGLLVDGTDLTPFCVNVTGFTADYTNTGQAAAFRADLSYQSGADAGTTLWTQRELRV
                     NDPLRLDGQRLYLLGHGFTPRFRVTYPDGEVRDYAQPFQPMDSAFTSDGAVKITDPPG
                     DGTRETRQLAIVGVFAPTAATTPAGALTSRFPAMTAPAVAIQVYRGNLGMEGGRPQSV
                     FAIDTSQVTRGAL"
BASE COUNT          867 a         2239 c         1893 g          909 t
ORIGIN      
        1 cgggtatagg aggcgggttc accgcgcatc cgcggtgact tccgacaccc cgtccgccac
       61 cttgcacaca aggagtgcct tctcatgacc accaaaacca gactcggcgt cgccgcgatc
      121 gcggcgtccc ttgccttcgt tctcgctgcc tgcagcggcg gtagccaatc cggcacagcg
      181 acatccgaca cgaccactgc tcggtcgacc ggctcgcccg cgtcgatcag cgccgccgcg
      241 accgcgacct tcaacgctgc ggacgttgcg ttcgcccaga tgatgatcgt gcaccaccag
      301 ggagcgatcc agatggccga tctggccccc agccgcgcgc agaatcagga cgttctggcc
      361 ctcgccgccc ggatcaaggc cgcgcaggcc ccggaaatcg accagatgac cagttggctc
      421 accgcttggg gtgccgcccc cagcatgatg tccggctcca cgaccagcgg cacgggcggc
      481 atggaccacg gcgggatggg cggggccacc tcggccgccg ggagttcgat gagcatgccc
      541 gggatgatgt ccggcgacca gatgcagcaa ctggagtcgg cgtccggggc cgccttcgac
      601 aagatgttcc tcgaactcat gatcgtgcac caccagggtg cgatcgagat ggccgagacc
      661 gagatcgcgg acggcagcaa ccccgaggcg gtcgcgctcg cccagaagat caagtccgat
      721 cagaccgcgg agatcgccga aattcaggcg ctgctgcaaa ccctgtagcc catcgggcgc
      781 tttcggtggg acccgcccgg ttcgatccgg gcaggggcgg gtcccaccgt cccggcagcg
      841 ggcactgcga ccggaaagga agctgctcga tgatcgcttc gtgggtgact cgtcgccccg
      901 cgcgcaccct tggtcttgtc gtggtcggtg cactgctgct cgcggcctgc tcggccggcg
      961 aggaatcggt ggtcgtcacc cagacggtga ccgaaggagc gggccgcccg atcaccgccg
     1021 caccgctgag cgggtcctcc tccgaaccac ccgaatccag ccaggcgtcg agggcccggt
     1081 tcagtgcctc ccctcggttc ggcagcaccg acgtcgcacc cacccagaag gtgaccgtga
     1141 ccgcgttcaa cgccaccatc acgcaggtca acgcggtctc cgacaccgac ggcaccgtgc
     1201 tggctggcgc catctccgag gacggtggca cctggacgct gaccgaccgg atggcctacg
     1261 ccaccagcta ccggatcacc ggcaccgcca gcacccgcga cgggtccacc gtgccgatcg
     1321 acgggcagct gagcaccgtc gaccccgggg acaccatgcg ggcctcgttc cagctcgccg
     1381 agggcggcac ctacggggtg gccaccccga tcatcctcac cttcgccggt caggtcaccg
     1441 acaaggccgc ggccgaggct gccctgagcg tcatgaccga caagggtgtg gtcgagggat
     1501 cctggggctg gctgcaggac gaggacatcc aaggcactgg ggtcaagcag tcccgcgtgc
     1561 actttcggac caaggactac tggccggcga acaccaaggt gacggtcgcc gccaacctgt
     1621 acgggatcga cctgggcggg gcctggggcc gtgaggacct gacccgcacg ttcaccatcg
     1681 gccggtccca gatcgtcacc gccgacgtca actcttaccg cctggtcgtc accgtcgacg
     1741 gtgcggtcac caagaactat cccgtctcct acggccgggc gaccgacaac ccggacctgg
     1801 agacccgcag cggcgtccac gtcgtccagg aaaagtacga cagcttcacc atgtgcaacc
     1861 ccaagtacgg gtattgcgga ttcgtcgcca actgggccgt ccggatcagc aacaacggcg
     1921 aattcatcca cgaaaacgcc gccgtcgtcg catccctggg caaggagaac gtctcccacg
     1981 gttgcgtcaa catgagcccc gccgacgcca aggactacta cgacagcgcc ctgtacggcg
     2041 accccgtcga ggtcaccggc accaccccgc agctcggccc gtccgacggc gacatcttcg
     2101 actggactta cccctacgac acctggaaga cgttctccgc cacatgatca cccaccccgc
     2161 cggccgcact gcgcccggaa ccgggcgcaa caaaccggcc ggccccgcgc ccacatcagc
     2221 accaaggcca agcccccggc cgccctcccg tgcgttcgtg accgccgcga cgatcctgct
     2281 gctcgccggc tgcagcgccc ccgtcgccag cacgccagct tccccggccg caaccgccca
     2341 gggagccagc tccggcctgc ccagccccca cgtccacgga atcgccatcg acccggccga
     2401 cgacaccgtc tacctggcca cccacgacgg gctgttcagc tacggcacgg gcacacccac
     2461 ccgggtgggc ccgatcatcg acctgatggg cttcgccgcc gccggcccga ccaccttcta
     2521 cgcctccggg caccccggcc ccggcaccgc actgccgaac ccggtcggct tgttgacctc
     2581 cgcagacgcc ggccgcacct ggcagccact gtcccggcag ggcgagtccg acttccatgc
     2641 cctgaccgcg gcaccgttcg gtgtgatcgc cgccgaccag gccctgcgga tcactgagga
     2701 cggccggtcg tggcgcgagc tgctgatccc ccaacccgcg atcgcgttgg ccgcagcacc
     2761 ggacggtggc acgatcctcg cggccaccga cgccggcccg ctgcggtcga caacccgcgg
     2821 cgacacctgg gcaccggtcg acggcgcgcc gccgctgcaa ctgctcgcct gggcggacgc
     2881 ggacaccgcg gtcggcacca cctacgacgg tgccgttcac atcagcgacg acgccggccg
     2941 cagctggtcc ccgcgggcgc gcgtcggacc cgcgcaggcc atcacggccc gggccggcgc
     3001 ggacggcaac gtgcgggtgc tcattgtcac tcgctccgag atcctcgaat cggcggacgg
     3061 cggcgtcacc ttccacccgt gggtgccgta accggacgcg tggtcgtccg gccggcctcg
     3121 ccctgaccgc gctggccctg tctactatgc ttcatagttg aatgttgccg cgcgcgggtc
     3181 atcggtcatc ggagggaagc gtcgaatgct gcttcgaccc ccagccggtc gccggacccg
     3241 gctgcgccgc tggatcgccg ccctcggttt ggcgttgccc ctggtgttga ccggatgcgg
     3301 cgccggcggc gggaacaacg cgttcacctt cgtctccccc ggtggccaga acgcgttctc
     3361 ctacccgtcc gggcaacggc agaccgtcgg tgacctgtcc gggcccagcc tgatggatga
     3421 cggcgaccgc atcgtgggta tgtccgacta tgccgaccgg gtggtggtgc tcaacgtgtg
     3481 gggctcctgg tgtgcacctt gccgcgccga ggcaccggat ctgacctccg ccgcgcggga
     3541 ccttgccccg ctgggggtgc agttcatcgg catcgacgtg cgggacaccc gtgccggtgg
     3601 tcgggacttc cacacgtcgt tcgacgtgcc ctatccctcc atcttcgacc ccagcatgcg
     3661 caccctgctg tccctgcgcg gctttccgac cagctcgatt ccgtcgacga tcgtgctgga
     3721 ccgcgagcat cgggtggccc agatctggtt gcgtcggatc accgccaccg aactgaccgc
     3781 caccgtcaga gccattgcca ccgaggccgc ctgacatggc atccctcgcc acggccgcca
     3841 ctctggtcac cgcacccgag cagaccatca ccgacgtcgt ggtgtccggg ccgctaaccc
     3901 tggccatcat gctggccttc gccgccggca ccgtgtcctt cgcctccccc tgctgcatcc
     3961 cgctggtgcc cgggtacctg tcgtatctgg ccgggctggt cggcgccgac accacccgcg
     4021 ccgccggcga aggcgacctg cgggtccggc gccgcgccgt gggcgcgacg ctgctgttca
     4081 tcgccgggtt caccgccgtg ttcctcgccc agaccgccgc ggtgctcggc gtcgcccatg
     4141 ccctgctcag cgacggcgag ctgctgatgc gggtcggcgg cacggtgacc atcgtgatgg
     4201 gtctggtcat gctcggcttc atccagccgc tgcaacgcga gttccgcatc cacgcccggc
     4261 ccaccggccc cgtcgccggg gcgctgctgc tcggcggcac cttcggcctg ggctgggtcg
     4321 tgtgcatcgg ccccaccctg gccgggatca tcgccctggc taccgccacc gattggggcg
     4381 gctcggcctg gcgcggcatg ctgctggtcg tcttctactg cctcgggctg gggttgccgt
     4441 tcctgctgct gtcgatcggc ctgggctggg cggccaccac ggtcggcttc ctgcgccggc
     4501 acagtaggac gctgcaggtc atcggcggag cgagcatggt catcctgggc gtgctgatgc
     4561 tcaccgggct gtggggtcag ttcgtggctt ggctgcaggt cgcgctgtcc ggctccaccc
     4621 gggtgtggtt gtgacccgcg ggaccgcgga cgataccgcg caaagacctg ggcggccgat
     4681 cgctcgagca ccgatgctgc cacgtcggtg gcggcgcatc ctggccccgg tgcgcaccct
     4741 gtggcgtcgg ctgaccagca tgcggaccgc actggtgctg ctgttcctgc tcgccgtggc
     4801 ggcgctgccc ggtgccctgc tgccgcagtg gaacctgaat cgggccaaga ccactcagta
     4861 cctgctcgac catcccgttc tcggcccctg gctcgaccgg ttggggttcc tggcggtgtt
     4921 ctccgcgccc tggttcgccg ccatctacct gctgttgttc acctccctcg tcggctgcct
     4981 cgttccccgg acgctggatc ttctccggcg gctgcggcgg ccaccgcccc ggacgccccg
     5041 ccacctgtcc cggctgcctc atcacgccat cagggaggtc gccggcccgc cggaggtggt
     5101 ggcgatccgg atcggccgcg gcctgcgcag gtggcgggtc aggcgaaccg tcgaggacac
     5161 cggcgccgtc agcatcgcgg ccgaaaaggg atacctgcgt gaactgggaa acctgctgtt
     5221 ccacctgtcg ctgctgggcc tgttggtggc gatggccgtc ggcaaactcg tcggctacga
     5281 gggatcggtc atcgtcgacg tcggtaacgg cttctgctcg gtcggcccaa cggtctacga
     5341 cgatttccgg cccggcctgc tggtcgacgg caccgacctg accccgttct gcgtcaacgt
     5401 caccgggttc accgccgact acaccaacac cgggcaggcc gccgcgttcc gcgcagacct
     5461 gagctaccag tccggcgctg atgccggcac caccctgtgg acccaacgtg agctgcgggt
     5521 caacgatccg ctgcgcctgg acgggcagcg actgtacctg ctcggccacg ggttcacccc
     5581 acggttccgg gtcacctacc cggacggcga ggtccgcgac tacgcccaac cgttccaacc
     5641 catggacagc gcgttcacct ctgacggcgc cgtgaagatc accgacccac ccggcgacgg
     5701 cacccgggag acccgccaac tggccatcgt cggagtgttc gcgccgaccg cggccaccac
     5761 ccccgccggg gcactgacct ccaggttccc ggcgatgacc gcgccggccg tggcgatcca
     5821 ggtgtatcgg gggaacctgg gcatggaagg tgggcggccc caatcggtct tcgccatcga
     5881 cacctcgcag gtcacccgcg gcgcgctg
//
LOCUS       DAOSUG010000142         5907 bp    DNA     linear   ENV 10-JUN-2024
DEFINITION  MAG TPA_asm: Micropruina sp. isolate AMR_MDS_2195
            NODE_6244_length_5907_cov_3.591422, whole genome shotgun sequence.
ACCESSION   DAOSUG010000142 DAOSUG010000000
VERSION     DAOSUG010000142.1
DBLINK      BioProject: PRJNA925477
            BioSample: SAMN32864262
            Sequence Read Archive: SRR4236673
KEYWORDS    WGS; ENV; Metagenome Assembled Genome; MAG; Third Party Data; TPA;
            TPA:assembly.
SOURCE      Micropruina sp. (wastewater metagenome)
  ORGANISM  Micropruina sp.
            Bacteria; Bacillati; Actinomycetota; Actinomycetes;
            Propionibacteriales; Nocardioidaceae; Micropruina.
REFERENCE   1  (bases 1 to 5907)
  AUTHORS   Abdulkadir,N., Saraiva,J.P., Zhang,J., Stolte,S., Gillor,O.,
            Harms,H. and Rocha,U.
  TITLE     Genome-centric analyses of 165 metagenomes show that mobile genetic
            elements are crucial for the transmission of antimicrobial
            resistance genes to pathogens in activated sludge and wastewater
  JOURNAL   Microbiol Spectr 12 (3), e0291823 (2024)
   PUBMED   38289113
REFERENCE   2  (bases 1 to 5907)
  AUTHORS   Abdulkadir,N.
  TITLE     Direct Submission
  JOURNAL   Submitted (24-MAR-2023) Environmental Microbiology, Helmholtz
            Center for Environmental Research -UFZ, Permoserstr 15, Leipzig
            04318, Germany
COMMENT     The annotation was added by the NCBI Prokaryotic Genome Annotation
            Pipeline (PGAP). Information about PGAP can be found here:
            https://www.ncbi.nlm.nih.gov/genome/annotation_prok/
            
            ##Genome-Assembly-Data-START##
            Assembly Method        :: SPAdes v. 3.11.1
            Genome Representation  :: Full
            Expected Final Version :: Yes
            Genome Coverage        :: Not Applicable
            Sequencing Technology  :: Illumina MiSeq
            ##Genome-Assembly-Data-END##
            
            ##Genome-Annotation-Data-START##
            Annotation Provider               :: NCBI
            Annotation Date                   :: 05/04/2023 04:57:10
            Annotation Pipeline               :: NCBI Prokaryotic Genome
                                                 Annotation Pipeline (PGAP)
            Annotation Method                 :: Best-placed reference protein
                                                 set; GeneMarkS-2+
            Annotation Software revision      :: 6.5
            Features Annotated                :: Gene; CDS; rRNA; tRNA; ncRNA
            Genes (total)                     :: 2,776
            CDSs (total)                      :: 2,750
            Genes (coding)                    :: 2,714
            CDSs (with protein)               :: 2,714
            Genes (RNA)                       :: 26
            tRNAs                             :: 24
            ncRNAs                            :: 2
            Pseudo Genes (total)              :: 36
            CDSs (without protein)            :: 36
            Pseudo Genes (ambiguous residues) :: 0 of 36
            Pseudo Genes (frameshifted)       :: 2 of 36
            Pseudo Genes (incomplete)         :: 32 of 36
            Pseudo Genes (internal stop)      :: 2 of 36
            ##Genome-Annotation-Data-END##
FEATURES             Location/Qualifiers
     source          1..5907
                     /organism="Micropruina sp."
                     /mol_type="genomic DNA"
                     /submitter_seqid="NODE_6244_length_5907_cov_3.591422"
                     /isolate="AMR_MDS_2195"
                     /isolation_source="Activated sludge of municipal
                     wastewater treatment plants"
                     /db_xref="taxon:2737536"
                     /environmental_sample
                     /geo_loc_name="Canada"
                     /lat_lon="49.25 N 123.1 W"
                     /collection_date="2015-06-16"
                     /metagenome_source="wastewater metagenome"
                     /note="metagenomic"
     gene            174..1226
                     /locus_tag="PLF56_06030"
     CDS             174..1226
                     /locus_tag="PLF56_06030"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_013160185.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_component: GO:0016020 - membrane [Evidence IEA];
                     GO_function: GO:0022857 - transmembrane transporter
                     activity [Evidence IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="iron chelate uptake ABC transporter family
                     permease subunit"
                     /protein_id="HOQ53157.1"
                     /translation="MTSVRPRPDTTPGRTPEAPRAFARGVGLPALLAALAVAVLAGLA
                     VGARTIPPLEVIRILLFEATGQNAMVIRDLRMPRTLIGLVAGVALGVAGGLIQAVTRN
                     PLADPGILGVNAGAAFAMAIGTGVAGVTGGLSTLLVAYAGAFGASLLVYAIGSLGRGG
                     GSPVRLILAGVALGAVLAGITRAIMLTDRQRFAGMIAWESGSLLDRDPSLLSVAGPFV
                     LLGLLLSLALAPSLNAIALGDDLASALGARVLRTRVLAVVAVMLLAGSATALAGPIAF
                     VGLMVPHAVRWIVGPDWRWILGYSVLAAPTLLILADVVGRVLIPPGELPAGIVTALIG
                     APVLIVLVRRRKASEL"
     gene            1223..2293
                     /locus_tag="PLF56_06035"
     CDS             1223..2293
                     /locus_tag="PLF56_06035"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_013160184.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_component: GO:0016020 - membrane [Evidence IEA];
                     GO_function: GO:0022857 - transmembrane transporter
                     activity [Evidence IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="iron chelate uptake ABC transporter family
                     permease subunit"
                     /protein_id="HOQ53158.1"
                     /translation="MSSTAQQVPGVDAAPIDPRARAVAVPVVGGWVARRGVGVAGAAL
                     VSALALAIVALGMGDLPLSPGEVVQALFATDGGFTSTVVRDWRLPRVLGALVFGSALA
                     LSGAVFQSLTRNPLGSPDVIGFATGAYTGALIALTAIGGGAVTASAGALVGGLGTALL
                     VYLLAYSGGMHGFRLIIVGIAVTAMLHAVNLFLLLRAQEEVAMAASIWGAGSLSLLSW
                     TSLVPALIGLLLCSPGLVLVPPLRQLELGDDAASAHGVNVEGARLGLVLFAVALVAIV
                     TAATGPIAFIALAAPQVARRLAGGAGIPLATSALTGAVLLLGADLIAQHALPVDLPVG
                     IVTVVLGGSFLLTLLIQEARGR"
     gene            2290..3093
                     /locus_tag="PLF56_06040"
     CDS             2290..3093
                     /locus_tag="PLF56_06040"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_014847126.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_function: GO:0005524 - ATP binding [Evidence IEA];
                     GO_function: GO:0016887 - ATP hydrolysis activity
                     [Evidence IEA];
                     GO_function: GO:0042626 - ATPase-coupled transmembrane
                     transporter activity [Evidence IEA];
                     GO_function: GO:0140359 - ABC-type transporter activity
                     [Evidence IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="ABC transporter ATP-binding protein"
                     /protein_id="HOQ53159.1"
                     /translation="MRAPVTGSRLRAEGVTLRYDQRVVSRDLSVEIPAGEFTVIIGPN
                     ACGKSTLLRSLSRLLRPAAGQVRLDGRPLASFKAKEFARTVGLLPQSSIAPEGITVVD
                     LVSRGRFPYQGIIRQWTDADEQAVRDAMAATNVADLATREVAELSGGQRQRVWAAMAI
                     AQQTPILLLDEPTTFLDLTHQIEMLDLFARLHRSGTTIVAVLHDLNQAARYATHLIVM
                     KDGAVVAADRPAQVLTEELVHEVFELNCLVIDDPASGTPLIVPTDGEWR"
     gene            complement(3176..4057)
                     /locus_tag="PLF56_06045"
     CDS             complement(3176..4057)
                     /locus_tag="PLF56_06045"
                     /inference="COORDINATES: protein motif:HMM:NF018677.2"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="sucrase ferredoxin"
                     /protein_id="HOQ53160.1"
                     /translation="MNPRCSVASEQRGEPLAGTASTVRHFLLMEDPGPWGPEILRSQR
                     LPGPVREPLLHWQRDYGVRPLLIRRPGRYLAGPRRVFVVNARHGWCQTGLVGSLAEVA
                     DLDLSGIAGGAGVGLAPHPDPVLLVCTHGRHDACCAERGRPLAAALAPRWPDLVWESS
                     HLGGDRFAGNLLILPEAHSYGRLTPERAPQVVADHLAGLVSLDHYRGHCTMPWPEQAA
                     EHALRSRLGLRALDGVRVQRLRRDGTLFSVTLAAAGRTYRVDVRHGARPPELLTCRST
                     QPSGAVAYELIALTRLN"
     gene            complement(4054..5175)
                     /locus_tag="PLF56_06050"
     CDS             complement(4054..5175)
                     /locus_tag="PLF56_06050"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_011757804.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="cupin domain-containing protein"
                     /protein_id="HOQ53161.1"
                     /translation="MNPLRLLSGDPETFINEVWGRRILVHQVEPGLLEGIFGIDQADH
                     LLTETALRTPQIRLAKSGSVLPESRFTKHATIAGQQLTGLIDARKLLDEFADGATIVF
                     QGLQRYWQPLRALVRGLEEQLGHPCQANAYLTPPGSQGFALHSDTHDVFVFQTFGAKQ
                     WEVHDDAGAHQVDMLPGVSMYLPTGTPHAARTQATASLHVTLGINRYVWRDLLKRVVD
                     PLLAAADDPLPAALFDDPDALASALRERLATLADAVRSTDAAAVVARHERSFATGRQP
                     LLGGALLDVLDAPAITDDTLLRRRAGSTCLLRTSGSSLDLLLGDRKVTVPARIEPAVR
                     AVLARGELRPADLHDHLDAAGALVLTRRLVREGLLEVVR"
     gene            complement(5178..5432)
                     /locus_tag="PLF56_06055"
     CDS             complement(5178..5432)
                     /locus_tag="PLF56_06055"
                     /inference="COORDINATES: ab initio
                     prediction:GeneMarkS-2+"
                     /note="Derived by automated computational analysis using
                     gene prediction method: GeneMarkS-2+."
                     /codon_start=1
                     /transl_table=11
                     /product="hypothetical protein"
                     /protein_id="HOQ53162.1"
                     /translation="MSDTPKESLSDEEILTDAVPGSSPVGDADGVDGDGTDGTDGDGT
                     DGTDGDGTDGTDGDGTDGTDGDGTDGTDGDGTDGTDGVNA"
     gene            complement(5540..>5907)
                     /locus_tag="PLF56_06060"
     CDS             complement(5540..>5907)
                     /locus_tag="PLF56_06060"
                     /inference="COORDINATES: protein motif:HMM:NF013866.2"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_function: GO:0016787 - hydrolase activity [Evidence
                     IEA]"
                     /codon_start=3
                     /transl_table=11
                     /product="alpha/beta hydrolase"
                     /protein_id="HOQ53163.1"
                     /translation="VIGGHSLGGAMAADYAARHADQLSGLILLAAYPPEGVDLTRTSF
                     GSLSLLAEHDQVAAAAKVQAGMTQLSPSSRLEVIPGAVHAFFGRYGPQQGDGVPTVRR
                     EQAEEAIIERIVDYLSRIA"
BASE COUNT          765 a         2051 c         2162 g          929 t
ORIGIN      
        1 cgcagcggtg agggtcgcga gcagggcggc ggcgagaacg cgaaaacgca tgggagtcct
       61 tcggtcgagg gggatcagag cgatcggaca gggacgagct gattgtcgga gcacgcggca
      121 cgcgggacct gcctggtgcg acatttaggc aaggctaccc tacgatcgag cccgtgacct
      181 ccgtccgtcc gagacccgac acgacgcccg gtcgcacccc cgaagcccct cgcgcgttcg
      241 cccgtggtgt ggggttgccg gcgttgctgg ccgcgctcgc cgtggcggtc ctggccgggc
      301 tcgcggtcgg cgcgcggacg atccccccgc tcgaggtgat ccgcattctg ctcttcgagg
      361 ccaccgggca gaacgccatg gtgatccgcg atttgcgcat gccgcgcact ctgatcgggt
      421 tggtggccgg ggtggcgctc ggcgtcgcgg gcgggttgat ccaggccgtc acccgcaatc
      481 cactggccga tcccggcatt ctgggcgtga acgcgggcgc cgccttcgcc atggctatcg
      541 gcaccggcgt ggcgggggtc accggcggcc tgagcaccct gctggtcgcc tacgccggcg
      601 cgttcggcgc ctcgctgctg gtctacgcga tcggttcgct cggacgaggg ggcggcagcc
      661 cggtgcggct gattctggcc ggcgttgcgc tgggcgccgt cctggccggc atcacccggg
      721 cgatcatgct gacggatcgg cagcgtttcg cgggcatgat cgcctgggag tcggggtcgt
      781 tgctcgaccg cgacccgagc ctgctcagcg tggccgggcc tttcgtgctg ctcgggctgc
      841 tgctgtcgct ggccctcgcc ccgtcgctga acgcgatcgc gctcggggac gatctcgctt
      901 cggccctggg cgcccgcgtg ctgcgtaccc gcgtgctggc cgtcgttgcg gtgatgctgc
      961 tcgcgggcag cgccaccgct ctggcggggc cgatcgcgtt cgtggggctg atggtgccgc
     1021 atgccgtccg gtggatcgtg gggccggact ggcgctggat tctgggttac tcggtgctgg
     1081 ccgcccccac cctgctgatt ctggccgacg tggtcggccg ggtactgatc ccgcccggtg
     1141 aactacccgc cggcatcgtg acggcactga tcggggcacc ggtgctgatc gtgctcgtgc
     1201 ggcgacgcaa ggcgagcgag ctgtgagttc caccgcacag caggtgccgg gggtggacgc
     1261 tgccccgatc gatccgcgtg cgcgagccgt cgcggtgccc gtggtcggcg gctgggtcgc
     1321 gcgacgcggc gtcggcgtgg caggagcggc cctggtatcg gcgctggcac tcgcgatcgt
     1381 cgcgctgggt atgggtgacc tgccgctctc gccgggcgag gtcgtccagg ccctgttcgc
     1441 caccgacggc ggcttcacca gcaccgtggt gcgcgactgg cggctccccc gggtgctggg
     1501 cgccctggtg ttcggcagcg ccctggcgct ctccggtgcc gtcttccagt cgctgacccg
     1561 caatccactc ggctcaccgg acgtgatcgg attcgccacc ggcgcctaca ccggtgcgct
     1621 gatcgcgctc accgcgatcg gtggcggcgc ggtgaccgcg tcggccggcg cactggtggg
     1681 cggcctgggc acggccctgc tggtgtatct gctcgcctac tccggcggca tgcacggctt
     1741 tcggttgatc atcgtcggca tcgcggtcac cgccatgctg catgcggtca acctgttcct
     1801 gctgctgcgg gcgcaggagg aggtggcgat ggcggcgtcc atctggggtg cgggatcgct
     1861 ctcgctgctg agttggacgt cactcgtacc cgccctgatc ggcctgctgc tctgctcccc
     1921 cggcctggtg ctggtgccgc cgcttcggca gctcgaactc ggggacgacg ccgcctccgc
     1981 ccacggcgtg aacgtcgagg gggcccggct gggactggtg ctgttcgccg tggctttggt
     2041 ggcgatcgtg accgccgcga ccggcccgat cgcgttcatc gcgttggccg cgccccaggt
     2101 ggcccgccgg ctggccggcg gtgccggcat acccctggca acgtccgcgc tgaccggtgc
     2161 cgtgctgctg ctcggcgccg acctgatcgc ccagcacgca ctgccggtcg acctaccggt
     2221 gggcatcgtc accgtcgtgc tcggcggcag tttcctactg accctgctga ttcaggaagc
     2281 gaggggccga tgagagctcc cgtcactggt tctcggctgc gcgccgaggg cgtcaccctg
     2341 cgctacgacc agcgggtggt gagtcgggat ctcagcgtcg agattccggc cggcgagttc
     2401 accgtgatca tcggacccaa cgcgtgcggc aagtccaccc tgctgcgctc gctgtcgcgg
     2461 ctgctgcgtc cggcggcagg gcaggtgcgg ctggacggac gtccgctggc ctcgttcaag
     2521 gccaaggagt tcgcgcgcac ggtggggctg ttgccccagt cgtccatcgc tccggagggc
     2581 atcacggtgg tcgacctggt cagccgcggg cgctttccgt accaggggat catccggcag
     2641 tggacggacg ccgacgagca ggcggtccgc gacgccatgg ccgcgaccaa cgtggccgac
     2701 ctggccaccc gtgaggtcgc cgagctgtcg ggcggccagc ggcagcgagt ctgggcggcg
     2761 atggcgatcg cgcaacagac cccgattctg ctgctggacg agccgacgac ctttctcgac
     2821 ctgacccacc agatcgagat gctcgacctg ttcgcccggc tgcatcgctc gggcaccacc
     2881 atcgtggcgg tgctgcacga cctcaaccag gccgcccgtt atgccaccca cctgatcgtg
     2941 atgaaggacg gcgcggtggt ggcggccgac cgtccggcgc aggtgctgac cgaagagctc
     3001 gtgcatgagg tgttcgaact gaactgcctg gtgatcgacg atccggcctc gggcaccccg
     3061 ctgatcgtgc cgacggacgg cgagtggcgc tgacctccgg ggggttacgc cgaacgcccg
     3121 cctaagtatc ttccgcctgc gaagctacgc gggcggaaga tacttaggcg ggcgttcagt
     3181 tcaggcgcgt gagcgcgatc agttcgtagg ccaccgcccc cgagggctga gtgctgcggc
     3241 aggtgagcag ctccggcgga cgggcgccgt gccgcacgtc gacccggtag gtgcgtccgg
     3301 cggcggccag cgtgaccgag aacagggtgc cgtcacggcg caggcgctgc acccgcaccc
     3361 cgtccaacgc gcgcaggccg agcctgctgc gcagggcgtg ttcggcggcc tgctccggcc
     3421 agggcatggt gcagtgaccg cggtagtgat cgaggctgac caggccggcc aggtgatcgg
     3481 ccaccacctg cggcgcccgc tcgggggtga gccgtccgta gctgtgggcc tccggcagga
     3541 tcagcaggtt gcccgcgaac cggtcgccgc ccaggtgcga gctctcccac accaggtcgg
     3601 gccagcgcgg ggccagggcc gcggccagcg gacgtccgcg ctcggcacag caggcgtcgt
     3661 gtcgtccgtg ggtgcagacc agcaacaccg ggtcggggtg gggtgcgagt ccgaccccgg
     3721 ccccgccggc gatgccggac aggtcgaggt cggccacctc ggccaggctg ccgaccaggc
     3781 cggtctggca ccagccgtgc cgggcgttga ccacgaacac ccggcggggg ccggccaggt
     3841 atcgtccggg ccggcggatg agcagcggac gtaccccgta gtcgcgctgc caatgcagca
     3901 acggctcacg caccgggccc ggcaaccgct ggctgcgcag aatctcgggc ccccacgggc
     3961 cgggatcctc catcagcagg aagtgccgaa cggtggaggc cgtgccggcc aggggttccc
     4021 cccgctgctc gctggccacc gagcagcgcg ggttcaccgg acgacttcca gcagcccttc
     4081 acggaccagg cgccgggtca gcacgagcgc gccggcggcg tccaggtgat cgtgcaggtc
     4141 ggcgggccgc agctcgccgc gggccagcac ggcacgcacg gccggctcga tccgcgccgg
     4201 cacggtcacc ttgcgatcac cgagcagcag gtcgagggac gacccggacg tacgcagcag
     4261 gcaggtgctc cccgcgcgcc gccgcagcag cgtgtcgtcg gtgatcgcgg gcgcatcgag
     4321 cacgtcgagc agggcgccgc cgagcaacgg ctggcgtccg gtcgcgaagc tgcgctcgtg
     4381 cctggccacc acggcagccg catcggtgct gcgcaccgcg tcggccaagg tggcgagccg
     4441 ctcgcgcagg gccgacgcga gcgcgtccgg atcgtcgaac aaagccgccg gcagcgggtc
     4501 gtcggccgca gcgagcagcg ggtccacgac gcgtttgagc aggtcgcgcc acacgtagcg
     4561 gttgatgccc agcgtgacgt gcagggacgc cgtggcctgc gtgcgcgcgg cgtgcggcgt
     4621 gccggtgggc aggtacatcg agacgccggg cagcatgtcg acctgatgcg cgccggcatc
     4681 gtcgtgcacc tcccactgct tggctccgaa ggtctggaaa acgaacacgt cgtgggtgtc
     4741 ggagtgcagc gcaaagccct gcgagccggg cggagtcagg taggcgttgg cctgacaggg
     4801 gtgaccgagt tgctcctcca gcccccgcac gagcgcgcgc aacggctgcc aatagcgctg
     4861 cagcccctgg aacacgatcg tggcgccgtc ggcgaactcg tcgagcagct tgcgggcgtc
     4921 gatcagcccg gtcagctgct ggccggcgat cgtggcgtgc ttggtgaacc gcgattcggg
     4981 cagcaccgag ccgctcttgg ccagccggat ctgtggggtg cgcagcgctg tttcggtgag
     5041 caggtggtcg gcctggtcga tgccgaagat gccttcgagc aggccgggct cgacctgatg
     5101 gacgaggatc cggcgacccc agacctcgtt gatgaaggtc tcggggtcgc cggagagcag
     5161 gcgaagcggg ttcatgctca ggcgttcact ccgtcggtgc catcggtacc gtcaccatcg
     5221 gtaccgtccg tgccgtcacc gtcggtgcca tccgtgccgt ctccgtcggt accgtccgtg
     5281 ccgtcgccgt cggtgccatc ggtaccgtca ccatcggtac cgtccgtgcc gtcaccatcg
     5341 acaccatcgg cgtcgccgac cggactggag ccgggcaccg cgtcggtcag gatctcttcg
     5401 tcggacaggc tttccttggg ggtatcgctc atgtcgctcc ttcgggatcg gtcgccatgg
     5461 cgcggcgaca aagggttgta gggcgaccat agcccggcgt ggcacccgcg accagccccc
     5521 caaaggtgag tggtcaggct cacgcgatcc gcgacaggta gtccacgatc cgctcgatga
     5581 tcgcctcctc cgcctgttcg cgacgcaccg tcggcacccc gtcgccctgc tggggtccgt
     5641 agcgtccgaa gaaggcatgc accgcgcccg gaatcacctc cagcctgctg gacggcgaca
     5701 gttgcgtcat gccggcctgc accttggccg cggcggcgac ctgatcgtgc tcggcgagca
     5761 gggacaggct gccgaagctc gtgcgcgtca ggtcgacccc ctcgggcggg tacgcggcca
     5821 gcagaatcaa tccgctgagc tggtcggcgt ggcgggctgc gtagtcggcc gccatcgcgc
     5881 cgcccagcga atgcccgccg atcacca
//
LOCUS       DAOSUG010000143         5899 bp    DNA     linear   ENV 10-JUN-2024
DEFINITION  MAG TPA_asm: Micropruina sp. isolate AMR_MDS_2195
            NODE_6253_length_5899_cov_4.528576, whole genome shotgun sequence.
ACCESSION   DAOSUG010000143 DAOSUG010000000
VERSION     DAOSUG010000143.1
DBLINK      BioProject: PRJNA925477
            BioSample: SAMN32864262
            Sequence Read Archive: SRR4236673
KEYWORDS    WGS; ENV; Metagenome Assembled Genome; MAG; Third Party Data; TPA;
            TPA:assembly.
SOURCE      Micropruina sp. (wastewater metagenome)
  ORGANISM  Micropruina sp.
            Bacteria; Bacillati; Actinomycetota; Actinomycetes;
            Propionibacteriales; Nocardioidaceae; Micropruina.
REFERENCE   1  (bases 1 to 5899)
  AUTHORS   Abdulkadir,N., Saraiva,J.P., Zhang,J., Stolte,S., Gillor,O.,
            Harms,H. and Rocha,U.
  TITLE     Genome-centric analyses of 165 metagenomes show that mobile genetic
            elements are crucial for the transmission of antimicrobial
            resistance genes to pathogens in activated sludge and wastewater
  JOURNAL   Microbiol Spectr 12 (3), e0291823 (2024)
   PUBMED   38289113
REFERENCE   2  (bases 1 to 5899)
  AUTHORS   Abdulkadir,N.
  TITLE     Direct Submission
  JOURNAL   Submitted (24-MAR-2023) Environmental Microbiology, Helmholtz
            Center for Environmental Research -UFZ, Permoserstr 15, Leipzig
            04318, Germany
COMMENT     The annotation was added by the NCBI Prokaryotic Genome Annotation
            Pipeline (PGAP). Information about PGAP can be found here:
            https://www.ncbi.nlm.nih.gov/genome/annotation_prok/
            
            ##Genome-Assembly-Data-START##
            Assembly Method        :: SPAdes v. 3.11.1
            Genome Representation  :: Full
            Expected Final Version :: Yes
            Genome Coverage        :: Not Applicable
            Sequencing Technology  :: Illumina MiSeq
            ##Genome-Assembly-Data-END##
            
            ##Genome-Annotation-Data-START##
            Annotation Provider               :: NCBI
            Annotation Date                   :: 05/04/2023 04:57:10
            Annotation Pipeline               :: NCBI Prokaryotic Genome
                                                 Annotation Pipeline (PGAP)
            Annotation Method                 :: Best-placed reference protein
                                                 set; GeneMarkS-2+
            Annotation Software revision      :: 6.5
            Features Annotated                :: Gene; CDS; rRNA; tRNA; ncRNA
            Genes (total)                     :: 2,776
            CDSs (total)                      :: 2,750
            Genes (coding)                    :: 2,714
            CDSs (with protein)               :: 2,714
            Genes (RNA)                       :: 26
            tRNAs                             :: 24
            ncRNAs                            :: 2
            Pseudo Genes (total)              :: 36
            CDSs (without protein)            :: 36
            Pseudo Genes (ambiguous residues) :: 0 of 36
            Pseudo Genes (frameshifted)       :: 2 of 36
            Pseudo Genes (incomplete)         :: 32 of 36
            Pseudo Genes (internal stop)      :: 2 of 36
            ##Genome-Annotation-Data-END##
FEATURES             Location/Qualifiers
     source          1..5899
                     /organism="Micropruina sp."
                     /mol_type="genomic DNA"
                     /submitter_seqid="NODE_6253_length_5899_cov_4.528576"
                     /isolate="AMR_MDS_2195"
                     /isolation_source="Activated sludge of municipal
                     wastewater treatment plants"
                     /db_xref="taxon:2737536"
                     /environmental_sample
                     /geo_loc_name="Canada"
                     /lat_lon="49.25 N 123.1 W"
                     /collection_date="2015-06-16"
                     /metagenome_source="wastewater metagenome"
                     /note="metagenomic"
     gene            23..322
                     /locus_tag="PLF56_06065"
     CDS             23..322
                     /locus_tag="PLF56_06065"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_016456775.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_component: GO:0005737 - cytoplasm [Evidence IEA];
                     GO_function: GO:0003677 - DNA binding [Evidence IEA];
                     GO_process: GO:0008150 - biological_process [Evidence
                     IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="YbaB/EbfC family nucleoid-associated protein"
                     /protein_id="HOQ53164.1"
                     /translation="MNALLAQAQAMQSQLARAQVELAAKQVLGSAGGDLVQVTVNGAG
                     ELLAVNIKPEAWDEDDPSTLGDLIVAAVRDANKKVAILAETSMPQLPDLGGLTGQ"
     gene            335..952
                     /gene="recR"
                     /locus_tag="PLF56_06070"
     CDS             335..952
                     /gene="recR"
                     /locus_tag="PLF56_06070"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_020391097.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_process: GO:0006281 - DNA repair [Evidence IEA];
                     GO_process: GO:0006310 - DNA recombination [Evidence IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="recombination mediator RecR"
                     /protein_id="HOQ53165.1"
                     /translation="MFEGPIQDLIDELARLPGIGPKGAQRIAFHILTADKADVERLSD
                     SLRHVAENVRFCERCFNISESETCRICRDPRRDQTAICVVEESKDVMAIERTNEFRGL
                     YHVLGGAISPIDNVGPGDLHIRELVIRLGDGTVTEVILATDPNLEGEATATYLSRLIL
                     PMGVTVSRLASGLPVGGDLEYADEVTLGRAFQGRRTLGDPAGARG"
     gene            945..1844
                     /locus_tag="PLF56_06075"
     CDS             945..1844
                     /locus_tag="PLF56_06075"
                     /EC_number="3.1.3.-"
                     /inference="COORDINATES: protein motif:HMM:NF019887.2"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_function: GO:0016787 - hydrolase activity [Evidence
                     IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="HAD family hydrolase"
                     /protein_id="HOQ53166.1"
                     /translation="MADATACPAEPLAERTYLLRPPERIRLISSDLDGTMLFEHRTIT
                     PRVRSALHACAQAGLPIVPNTGRQHLKLASLLGEAGGPVGLAVTNNGAVGVDLASGEI
                     LFEELIHPDAQLYLGERLTATLPDVRFWALRSGARESVLQHGYRELMNEQEIVHGGPI
                     GDSAPLPEVLAEPAIKLIVRHPLMPPHQLLHEIQRLDLPGFHATTSGAPFVEVQGLGV
                     TKASGIARLTQHLGVEPREVLAIGDELNDIEMIRWAGYGVAMGNALPQVKAAADAVTA
                     PNSADGLALVLETLLAVRELTAS"
     gene            1926..2330
                     /locus_tag="PLF56_06080"
     CDS             1926..2330
                     /locus_tag="PLF56_06080"
                     /inference="COORDINATES: ab initio
                     prediction:GeneMarkS-2+"
                     /note="Derived by automated computational analysis using
                     gene prediction method: GeneMarkS-2+."
                     /codon_start=1
                     /transl_table=11
                     /product="hypothetical protein"
                     /protein_id="HOQ53167.1"
                     /translation="MGPRGGGGGFGFGFLHVIGSLIGMALFLALLALLAGLVFKVAVK
                     KGWVRKPRHHGHGPHGHRPEPGGPSPQHPHEALRILDERLARSEIEIDDYKARRDALT
                     GNSFPPFHQPPQPPVEPPTVTDEGAPHVPPAP"
     gene            complement(2394..2747)
                     /locus_tag="PLF56_06085"
     CDS             complement(2394..2747)
                     /locus_tag="PLF56_06085"
                     /inference="COORDINATES: protein motif:HMM:NF020397.2"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="DUF1801 domain-containing protein"
                     /protein_id="HOQ53168.1"
                     /translation="MADQPTSVDQYIDGFSPEVRSRLIRVRELLLQVMPDAEQTIRYG
                     MPALMLSGRYGLHFAGWKKHIGLYPVPVFDGELEAEIQPLRSGKDSVTLAHAKPLPEH
                     LITRIAQEIVGRRAG"
     gene            2923..3771
                     /locus_tag="PLF56_06090"
     CDS             2923..3771
                     /locus_tag="PLF56_06090"
                     /inference="COORDINATES: ab initio
                     prediction:GeneMarkS-2+"
                     /note="Derived by automated computational analysis using
                     gene prediction method: GeneMarkS-2+."
                     /codon_start=1
                     /transl_table=11
                     /product="hypothetical protein"
                     /protein_id="HOQ53169.1"
                     /translation="MANPDPRAVPPVPDAPSAPLPNGRSTRTHLLSNGVVALLLGLGL
                     ASAVRLLAQWVMLAALGMGDGGLNSVESLPRLVRDNALVAGAGPVVGLLLGLAMQVLQ
                     PFRFRGDFAHLLWIWTACSSLMLAAVPLACAPFGGDLSVVLVALRLPAWVGGVAVAAG
                     VAAVLGVAHQWAIHAVRLCGREVSRLLCFSVYPWLIGTVVQAAFVLTVVYLDRLDIPR
                     TQALVPLLGGAALTAFAPLSLLFTRRTDELEEPLLVGRVPWVAVLALVLLAAGFGAAR
                     AGLGIA"
     gene            4194..>5899
                     /gene="ileS"
                     /locus_tag="PLF56_06095"
     CDS             4194..>5899
                     /gene="ileS"
                     /locus_tag="PLF56_06095"
                     /EC_number="6.1.1.5"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_014847784.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_component: GO:0005737 - cytoplasm [Evidence IEA];
                     GO_function: GO:0004822 - isoleucine-tRNA ligase activity
                     [Evidence IEA];
                     GO_process: GO:0006428 - isoleucyl-tRNA aminoacylation
                     [Evidence IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="isoleucine--tRNA ligase"
                     /protein_id="HOQ53170.1"
                     /translation="MNEYRPVPPQVNLPAMEHQILDLWDARDTFATSLAATQGGPAWT
                     FYEGPPTANGTPGTHHIEARVFKDVFPRFKTMQGFHVDRKAGWDCHGLPVELAVEAEL
                     GFNGKPDIEAYGVEAFNAKCRESVTRHVDEFTGLTRRMGYWVNFDQAYWTMDASYVES
                     VWWALKQIFDAGLLVEDYRVAPYCPRCGTTLSDHELAQGYEDVTDPSVYVRFPLISGP
                     WAGKASLLVWTTTPWTLVSNTAVAVHPDMTYVVATKLGDDPETLVVAEPLVDKVLGEG
                     WQIEATLAGRELEFWTYQRPFELIDFPDVVGGTHYVVLADYVTTEDGSGLVHQAPAFG
                     EEDMAVCRAYGLPMVNPIRPDGTFEPGIEKVGGVFFKTADATLVADLVARGLMYRVLD
                     YEHSYPHCWRCHTVLLYYAQPSWYIRTTQIKDALLRENEATNWYPDTIKHGRYGDWLN
                     NNIDWALSRSRYWGTPLPIWRCPNGHLHCVGSRAELSELSGEDQAALDPHRPFVDEVS
                     FACPNCHETAHRVPEVIDAWFDSGSMPFAQWGYPWAEGSTQTFENAYPADFICEAIDQ
                     TRG"
BASE COUNT          901 a         1980 c         2044 g          974 t
ORIGIN      
        1 gttcggcgag cagggattcg acatgaacgc gctgctggcg caggctcagg ccatgcagtc
       61 gcagctggcc cgcgcccagg tagagctggc cgccaagcag gtgctcggct cggccggcgg
      121 agacctggtg caggtcaccg tcaacggagc cggcgagctg ctggcggtga acatcaagcc
      181 cgaggcgtgg gacgaggacg acccgtccac cctgggtgac ctgatcgtgg ccgccgtacg
      241 cgacgccaac aagaaggtcg cgatcctggc cgagaccagc atgccccagc tgccggatct
      301 cggcggcctc accggtcagt aggccgggtc cacggtgttc gaaggcccga ttcaagacct
      361 gatcgacgag ttggcccggc tgccaggcat cgggccgaag ggtgcccaac gcatcgcctt
      421 tcacattctg accgcagaca aggccgacgt ggagcggctg tccgactcgt tgcggcacgt
      481 cgccgagaac gtgcgcttct gcgagcgttg cttcaacatc tctgaatccg agacctgccg
      541 catctgccgc gacccgcggc gtgaccagac ggcgatctgc gttgtcgaag agtccaaaga
      601 cgtgatggcg atcgaacgca ccaacgagtt ccggggcctc tatcacgtgc tcggcggtgc
      661 gatttcgccg atcgacaacg tcggccccgg tgatctgcac attcgtgagc tggtgatccg
      721 gctgggcgac ggcacggtca ccgaggtgat cctcgccacc gaccccaacc tcgagggcga
      781 ggcgacagcc acgtacctgt ccaggctgat ccttccgatg ggggtcacgg tgtcgcggct
      841 ggcgtcgggg ctgccggtcg gcggcgacct cgaatacgcg gacgaggtga cgctcggccg
      901 agccttccag ggccgccgaa cgctgggcga cccggcgggc gcccgtggct gacgcgacag
      961 cgtgccctgc agaacccctt gccgagcgca cctacctgct tcgtccaccg gaacgcattc
     1021 gcctgatcag ctccgatctc gacggcacca tgctgttcga gcatcgcacc atcaccccgc
     1081 gcgtccgctc ggcgctgcac gcctgcgcgc aggccggcct gccgatcgtc ccgaacaccg
     1141 gacgccagca cctcaagctc gcgtcgttgc tcggcgaggc gggtggaccg gtcgggctgg
     1201 cggtcaccaa caacggcgcg gtcggggtcg atctggcatc gggcgagatc ctgttcgagg
     1261 agctgatcca tccggacgcg cagctgtacc tgggcgagcg gttgaccgcc acgctgccgg
     1321 acgtgcggtt ctgggctctg cgctcggggg ctcgcgagtc tgtgctgcag cacggctacc
     1381 gcgagttgat gaacgagcag gagatcgtgc acggcggtcc gatcggcgac tcggcgccgc
     1441 tgcccgaggt gctggccgag ccagccatca agctgatcgt gcgacacccg ctgatgccgc
     1501 cgcaccaact gctgcacgag attcagcgac tggacctgcc tggctttcac gcgaccacgt
     1561 ccggcgctcc gttcgtcgag gtgcagggcc tcggcgtgac caaggccagc gggatcgctc
     1621 ggctgacgca gcacctgggg gtcgagccgc gcgaggtgct cgccatcggt gacgaactca
     1681 acgacatcga gatgatccga tgggcgggtt acggcgtcgc catgggcaat gcgctgccgc
     1741 aggtgaaggc ggcggcggac gccgtcacgg cccccaactc ggcagacggt ctcgccctgg
     1801 tgctggagac cctgctcgcg gtgcgcgaac tcacagcctc ttgacagttg ccttcgagcg
     1861 atcggcacgc actatggact gcagcaacga cgcatgagag caacgaggca gcagaggagc
     1921 aggtaatggg tcccaggggt ggcggcggag gcttcggctt cggctttctg cacgtgatcg
     1981 gcagtctgat cggaatggcg ctgttcctgg cgctgctggc attgctggcc ggtttggtgt
     2041 tcaaggtggc ggtcaagaag ggctgggtgc gcaagccccg ccaccacggg cacgggccgc
     2101 acgggcatcg tcccgagcct ggtggcccct cgccgcagca tccgcacgag gcgctgcgca
     2161 tcctggacga gcggctggcc cgcagcgaga tcgagatcga tgactacaag gcgcgtcgcg
     2221 atgcactcac cggcaacagc tttccgccgt tccatcagcc cccgcagccg ccggtcgagc
     2281 cgccgacagt gacggacgag ggcgccccgc acgttccgcc ggcgccgtag cacctcgcac
     2341 tgatcgaccg gacgatgccg tcacgatcag cgtggatccg ggcttagccg ggcttagccg
     2401 gcgcgccgtc cgacgatctc ttgggcgatc cgggtgatca ggtgctcggg taggggcttg
     2461 gcgtgcgcga gcgtcacgct gtccttgccc gagcgcaacg gctgaatctc ggcttccagt
     2521 tcgccgtcga acaccggcac ggggtagagg ccgatgtgtt tcttccagcc ggcgaagtgc
     2581 agaccgtatc gtccgctcag catcagggcg ggcatgccgt accggatcgt ctgctcggca
     2641 tcgggcatca cctgaagcag aagctcccgc acccgaatga gccggcttcg cacctcgggt
     2701 gaaaatccat caatgtactg atcgacggac gtcggttgat cggccatggc tcgccctcct
     2761 tcaatccgac cggcctgcac gaggcgatgc tcccacagcg catcatgggt gccaacagac
     2821 tgcgaggcac ggtagatccc gaaagggcgg ctggcgagcc gaaccgtgtc gcggggcggc
     2881 cggcggtcac agcggcttat cgtgtcgtgc tacgttccgg tcatggccaa ccccgacccc
     2941 agggccgtgc cgcccgtgcc ggacgcccct tcggcgcctc tccccaacgg ccgttccacc
     3001 aggacgcacc ttctgagcaa cggtgtggtg gccctgctgc tggggctggg gcttgcctct
     3061 gcggtccggc tgctcgcgca gtgggtgatg ctcgccgccc tgggtatggg cgacggcgga
     3121 ctcaattcgg ttgagtctct gccccggctc gtccgcgaca atgcactggt cgccggcgcc
     3181 ggcccggtgg tgggcctcct gctgggtctg gcgatgcagg ttctgcagcc cttccggttc
     3241 cgcggcgact tcgcccatct gctttggatc tggacggcct gcagctcgct gatgctggcc
     3301 gccgtcccat tggcgtgcgc accgttcggc ggcgacctgt cggtcgtgct ggtcgccctg
     3361 cgcctacctg cgtgggtggg cggggtggcg gtcgcggcag gcgtcgcggc ggtgctgggg
     3421 gtggcccacc agtgggcgat ccacgcggtg cggctctgcg gtcgcgaggt ctcgcggttg
     3481 ctttgcttca gcgtctaccc gtggctgatc ggcacggtgg tgcaggcggc gttcgtgctg
     3541 actgtggtct acctcgaccg gctggacatc cctcgcacgc aggccctggt gccgcttctg
     3601 ggtggtgcgg cgctgaccgc gttcgccccg ctgtcgctgc tgttcacccg ccgcaccgac
     3661 gaactcgaag aaccgctgct ggtcggcagg gtgccgtggg tcgccgtcct ggccctggtg
     3721 ctgctggcgg cggggttcgg cgcggcccgc gccggtctcg gcatcgcctg aagcctggac
     3781 gccgccccgc atgccgtttc atccgggcgc aggtgagcca ggatccgggc tcaggtgagt
     3841 tggagtcgcg gctcgggtga gttggaatgg gggctcgggt gagctgggtt ccgggcgcag
     3901 gtgagccggg atccgcgctc aggtgggtca gaatccgggc acccgtcgat tcaggttgag
     3961 cccgcatccg gggtaagctg ggcgactgtc gcacggcgtt gatggggcca tcaccccgaa
     4021 gctgccggaa gaacggcggg ccccgctggg gtaccgctca ctagaaccgg ccgcggcaag
     4081 cacgagcggg tcgtcccacg gggcggccaa gaagggtggt accgcggggt tcgcctcgtc
     4141 ccttcgtcct gggccgggtc cgtccggcga agcagtcgac gaaggaatga tcgatgaacg
     4201 agtatcgtcc ggtgccgccg caggtgaacc tgcccgcaat ggagcaccag atcctcgacc
     4261 tgtgggacgc gcgcgacacg ttcgccacct cgctggcggc cacccagggc ggccccgcct
     4321 ggaccttcta cgagggccca ccgaccgcca acggcacccc cggcacgcac cacatcgagg
     4381 ctcgggtctt caaggacgtc tttccccggt tcaagaccat gcagggcttt cacgtcgacc
     4441 gcaaggccgg ctgggactgc cacggcctac ccgtcgaact cgcggtcgag gccgaactgg
     4501 gcttcaacgg caagcccgac atcgaggcgt acggcgtcga ggcgttcaac gccaagtgtc
     4561 gcgaatcggt cacccggcac gtcgacgagt tcaccgggct cacccgccgg atgggctact
     4621 gggtgaactt cgaccaggcc tactggacga tggacgcctc ctacgtcgag tcggtgtggt
     4681 gggcgctgaa gcagatcttc gatgccggcc tgctggtcga ggactaccgc gtcgccccgt
     4741 actgcccgcg ctgcggcacc acgctgagcg atcacgaact ggcccagggc tacgaagacg
     4801 tcacggaccc gtccgtgtac gtccgctttc cgctgatttc cgggccgtgg gccggcaagg
     4861 cgtccctgct ggtctggacg acgacgccct ggacgctggt cagcaacacc gccgtcgcgg
     4921 tgcatcccga catgacctac gtggtggcga cgaagctggg cgatgaccct gagacgttgg
     4981 tggtcgccga acccctggtc gacaaggtgc tcggcgaggg ttggcagatc gaggcgacac
     5041 tggccggacg agagctggag ttctggacct accagcgtcc gttcgagctg atcgattttc
     5101 cggacgtcgt gggcggcacc cattatgtgg tgctggccga ctatgtgacc accgaggacg
     5161 gctcgggcct ggtgcatcag gcacccgcgt tcggcgagga ggacatggcg gtctgccgcg
     5221 cctacgggtt gccgatggtg aacccgatcc gtccggacgg cacgttcgag ccgggcatcg
     5281 agaaggtcgg cggggtgttc ttcaagaccg ccgacgccac cctggtcgcc gatctggtgg
     5341 cgcgcggcct gatgtaccgg gtgctcgact acgagcacag ctacccgcac tgctggcgct
     5401 gccacaccgt gctgctgtac tacgcacagc ccagttggta catccgcacc acccagatca
     5461 aggacgcgct gctgcgcgag aacgaagcca ccaactggta ccccgacacg atcaagcacg
     5521 gacgctacgg cgactggctg aacaacaaca tcgactgggc cctgtcgcgt tcgcgctact
     5581 ggggcacccc gctgccgatc tggcgctgcc cgaacggcca cctgcactgc gtcggctcgc
     5641 gcgccgaact ctcggagctg agcggcgagg accaggcggc gctcgacccg caccgtccgt
     5701 tcgtggacga ggtgagtttc gcctgcccga actgccacga gaccgcgcat cgggtgcccg
     5761 aggtgatcga cgcctggttc gactcgggct cgatgccgtt cgcgcagtgg ggctacccgt
     5821 gggcggaggg ttcgacgcag acgttcgaga acgcctatcc ggccgatttc atctgtgagg
     5881 cgatcgacca gacccgcgg
//
LOCUS       DAOSUG010000144         5886 bp    DNA     linear   ENV 10-JUN-2024
DEFINITION  MAG TPA_asm: Micropruina sp. isolate AMR_MDS_2195
            NODE_6268_length_5886_cov_3.857314, whole genome shotgun sequence.
ACCESSION   DAOSUG010000144 DAOSUG010000000
VERSION     DAOSUG010000144.1
DBLINK      BioProject: PRJNA925477
            BioSample: SAMN32864262
            Sequence Read Archive: SRR4236673
KEYWORDS    WGS; ENV; Metagenome Assembled Genome; MAG; Third Party Data; TPA;
            TPA:assembly.
SOURCE      Micropruina sp. (wastewater metagenome)
  ORGANISM  Micropruina sp.
            Bacteria; Bacillati; Actinomycetota; Actinomycetes;
            Propionibacteriales; Nocardioidaceae; Micropruina.
REFERENCE   1  (bases 1 to 5886)
  AUTHORS   Abdulkadir,N., Saraiva,J.P., Zhang,J., Stolte,S., Gillor,O.,
            Harms,H. and Rocha,U.
  TITLE     Genome-centric analyses of 165 metagenomes show that mobile genetic
            elements are crucial for the transmission of antimicrobial
            resistance genes to pathogens in activated sludge and wastewater
  JOURNAL   Microbiol Spectr 12 (3), e0291823 (2024)
   PUBMED   38289113
REFERENCE   2  (bases 1 to 5886)
  AUTHORS   Abdulkadir,N.
  TITLE     Direct Submission
  JOURNAL   Submitted (24-MAR-2023) Environmental Microbiology, Helmholtz
            Center for Environmental Research -UFZ, Permoserstr 15, Leipzig
            04318, Germany
COMMENT     The annotation was added by the NCBI Prokaryotic Genome Annotation
            Pipeline (PGAP). Information about PGAP can be found here:
            https://www.ncbi.nlm.nih.gov/genome/annotation_prok/
            
            ##Genome-Assembly-Data-START##
            Assembly Method        :: SPAdes v. 3.11.1
            Genome Representation  :: Full
            Expected Final Version :: Yes
            Genome Coverage        :: Not Applicable
            Sequencing Technology  :: Illumina MiSeq
            ##Genome-Assembly-Data-END##
            
            ##Genome-Annotation-Data-START##
            Annotation Provider               :: NCBI
            Annotation Date                   :: 05/04/2023 04:57:10
            Annotation Pipeline               :: NCBI Prokaryotic Genome
                                                 Annotation Pipeline (PGAP)
            Annotation Method                 :: Best-placed reference protein
                                                 set; GeneMarkS-2+
            Annotation Software revision      :: 6.5
            Features Annotated                :: Gene; CDS; rRNA; tRNA; ncRNA
            Genes (total)                     :: 2,776
            CDSs (total)                      :: 2,750
            Genes (coding)                    :: 2,714
            CDSs (with protein)               :: 2,714
            Genes (RNA)                       :: 26
            tRNAs                             :: 24
            ncRNAs                            :: 2
            Pseudo Genes (total)              :: 36
            CDSs (without protein)            :: 36
            Pseudo Genes (ambiguous residues) :: 0 of 36
            Pseudo Genes (frameshifted)       :: 2 of 36
            Pseudo Genes (incomplete)         :: 32 of 36
            Pseudo Genes (internal stop)      :: 2 of 36
            ##Genome-Annotation-Data-END##
FEATURES             Location/Qualifiers
     source          1..5886
                     /organism="Micropruina sp."
                     /mol_type="genomic DNA"
                     /submitter_seqid="NODE_6268_length_5886_cov_3.857314"
                     /isolate="AMR_MDS_2195"
                     /isolation_source="Activated sludge of municipal
                     wastewater treatment plants"
                     /db_xref="taxon:2737536"
                     /environmental_sample
                     /geo_loc_name="Canada"
                     /lat_lon="49.25 N 123.1 W"
                     /collection_date="2015-06-16"
                     /metagenome_source="wastewater metagenome"
                     /note="metagenomic"
     gene            complement(<1..774)
                     /locus_tag="PLF56_06100"
     CDS             complement(<1..774)
                     /locus_tag="PLF56_06100"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_013864720.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="DEAD/DEAH box helicase"
                     /protein_id="HOQ53171.1"
                     /translation="MSQSVLTEPTFASLGVPTSLVAALRDQGIETPFPIQSATLPDTL
                     AGRDVLGRGRTGSGKTLAFALPLVARLAGLVSSTGSIAQRTRPGAPRALILAPTRELA
                     NQILATLEPLAKAAGLQAMTIFGGVGQAKQVAQLRRGADIVVATPGRLEDLIGQAHVR
                     LDAVAITVLDEADHMADLGFLPAVTRILTGTPAGGQRLLFSATLDRGVDTLVKRFLSR
                     PLVHSVDSETSPVATLTHHVFLVGDATQKRQIVRELAGGA"
     gene            1295..1498
                     /locus_tag="PLF56_06105"
     CDS             1295..1498
                     /locus_tag="PLF56_06105"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_012181172.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_component: GO:0005829 - cytosol [Evidence IEA];
                     GO_function: GO:0001072 - transcription antitermination
                     factor activity, RNA binding [Evidence IEA];
                     GO_function: GO:0003676 - nucleic acid binding [Evidence
                     IEA];
                     GO_function: GO:0003723 - RNA binding [Evidence IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="cold-shock protein"
                     /protein_id="HOQ53172.1"
                     /translation="MATGTVKWFNSEKGFGFIAPDDGSADVFAHFTAIVGTGRRDLFE
                     GQQVEFETQRGQKGLQATNIRAL"
     gene            complement(1926..3035)
                     /locus_tag="PLF56_06110"
     CDS             complement(1926..3035)
                     /locus_tag="PLF56_06110"
                     /EC_number="1.14.19.-"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_020387274.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_component: GO:0016020 - membrane [Evidence IEA];
                     GO_function: GO:0016717 - oxidoreductase activity, acting
                     on paired donors, with oxidation of a pair of donors
                     resulting in the reduction of molecular oxygen to two
                     molecules of water [Evidence IEA];
                     GO_function: GO:0046872 - metal ion binding [Evidence
                     IEA];
                     GO_process: GO:0006633 - fatty acid biosynthetic process
                     [Evidence IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="acyl-CoA desaturase"
                     /protein_id="HOQ53173.1"
                     /translation="MSATDVPAPPRQRSARGEAVRVEWERRTNSFTELAARVREQGLN
                     RRHRWFYFALLGGLVVALAGAFTGMALLGDSWFQLLIAAALGVIFTQFAFVGHEASHR
                     QVFTSGRANDRLGRVVATFFVGISYAWWMTKHNRHHGNPNQVGRDPDIAPGWVAFTED
                     DAAVATGFRGWLNRRQGWLFFPLLPLLGLDLHQASIGRLFRRGKLEGRAQELSWLALR
                     FSLYLGVVFWLLPIGLAFAFLGVQVAVFGTYMGLSFAPNHIGMPIVPHGAKLDFLDKQ
                     VLTSRNVSGGWWMTMLMGGLNYQIEHHLFPSMPRLHLAKARRLVREHCRRHDVPYAEN
                     NIVQAMTIVVRYINQVGWAARASFSCPAAASMGRF"
     assembly_gap    3298..3397
                     /estimated_length=100
                     /gap_type="within scaffold"
                     /linkage_evidence="paired-ends"
     gene            complement(4026..5354)
                     /locus_tag="PLF56_06115"
     CDS             complement(4026..5354)
                     /locus_tag="PLF56_06115"
                     /inference="COORDINATES: protein motif:HMM:NF012873.2"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="condensation domain-containing protein"
                     /protein_id="HOQ53174.1"
                     /translation="MRLTNVSRMVLPPGRMMSYGVRLSDAMGRPLPVSFDQGRHVGQG
                     QRPGSWMAIAARVPAGTTPAHLQQAWWAVVQRHGTLRTGFGLDGEGRVVLHEFEAVPG
                     EWREHPIADGVDPREVLRRLFDAACAPFEQPSHLLVVMQPDADQADARPMVIVGSDHS
                     HVDMWSFLIVLRDLCTCLDDLHAGRPPGVGLAPVPAFAEHSAVLEARPPAPAEVHERW
                     AGLLAAEDGVMPRFPLPLGNPAFEGDSLVEVRDLLDAGQTARFGALAAQQGVRSIALA
                     LSVIVTATRDVADAPLRVLFPVHSRDEVRWRESVGWFITNSVLECADPDPAAGMSAVK
                     EAVALGSYPLAPIFAPYGGMPTAPGMFALSWLDTRRLPELPVDAQVQYVSAVIHDDGV
                     MVWFIVNDTGLHLRARYPNTAAARAHVVPWLDAVERGLLAMVRTEDRLTV"
     gene            5411..>5886
                     /locus_tag="PLF56_06120"
     CDS             5411..>5886
                     /locus_tag="PLF56_06120"
                     /inference="COORDINATES: protein motif:HMM:NF023568.2"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="alpha/beta fold hydrolase"
                     /protein_id="HOQ53175.1"
                     /translation="MLSVRDALLDYDVTGESGPIVVQLHGLTSCRERDTALGLNLARA
                     LGGHRIVRYDARGHGRSSGTADEASYSWPALAGDLLALLDTLSPDAPVHGVGTSMGSA
                     TLLHAAVRAPKRFASLTLAVPPTAWETRRAKAEAYRANADLVQREGVQAFVDLGGET"
BASE COUNT          983 a         1968 c         1985 g          850 t
ORIGIN      
        1 ggcgcctccg gccagctcgc ggacgatctg ccgcttctga gtggcatcgc cgaccaggaa
       61 cacgtggtgg gtgagggttg cgaccggcga ggtctcggag tcgacgctgt gcaccagcgg
      121 acggctcagg aaccgcttca ccaaggtgtc gacaccacgg tcgagggtcg ccgagaacag
      181 caggcgctgt ccgcccgccg gggtgccggt cagaatgcgg gtgacggcgg ggagaaagcc
      241 gagatcggcc atgtggtcgg cctcgtccag cacggtgatc gcgaccgcgt ccagccggac
      301 gtgggcctga ccgatcaggt cttcgaggcg gccgggtgtg gccaccacga tgtcggcgcc
      361 gcgccgcagc tgggcgacct gcttggcctg gccgactccg ccgaagatgg tcatcgcctg
      421 caggcccgcg gccttggcca gcggttcgag cgtggccaga atctgattgg ccagctcgcg
      481 ggtgggagcc aggatcagcg ctcgcggcgc gcccggacgg gtgcgctgcg cgatcgaccc
      541 ggtggacgac accaggcccg ccagccgcgc caccaggggc agggcgaagg ccagcgtctt
      601 gccggagccg gtgcggccgc ggccgagcac atctcgtccg gccagtgtgt cgggcagggt
      661 ggccgactga atcgggaaag gggtttcgat gccctgatcg cgcagggcgg cgaccaggga
      721 ggtggggacg ccgagcgagg caaaggtggg ctcggtcagg acggattgcg acaaagtact
      781 gctctcttgg acgaggggca ccacgatcct gggtccactc gcgaacctgg gcgatgaagc
      841 gccggcgcgt tgcggacggt gggtgcgatg cggcgcgcaa ggtgcgcacc atgcgtccat
      901 tgtcgcacga gttcacctgg acggcgctat cgcaccatcg gggcatgcct gctagagtga
      961 ttcgtcgcac cattttggtg ccaccgaatg tccagcccga gttggttctg gtgccaggag
     1021 ccaggccacc accggcagta gcgcctgtaa tgacagggtc gagcagccac accactcccc
     1081 cgtccgtccc gcgttccaga ccactcggtc ttcaccgcct caccgaaagc cagccagatc
     1141 agtaccgctg tacgcctcgc tcccgccgcc ccactgggtg gtggcgctca cgcgcgcggt
     1201 catggccggc acacccatgg attcctctcc ggcgccgtcg aagaggtcag tagggcagca
     1261 atcggcagcc caagaaacac gaaggaataa cgacatggcc actggcactg tcaaatggtt
     1321 caactccgag aagggcttcg gcttcatcgc tcccgacgac ggctctgccg acgtctttgc
     1381 gcacttcacg gctatcgtcg gcaccggccg tcgcgacctt ttcgagggtc agcaggtcga
     1441 gttcgaaacc cagcgtggac agaagggcct tcaggccacc aacatccgcg ccctgtgagc
     1501 aacagggtgg cgtgaggcgg ctcgccggct cagtcccccc atcccggacg ggtacaccgt
     1561 ccgctcactt gcgttgatca caacccggcg tgtgagccgt gcatgtcgca cggttcatac
     1621 cccgggttgt gtgctgttcg gcgacgtctg ttcaccgttg agggccgtca cgaggttcga
     1681 agcacagtgg gccgtcgaaa cggccacgag ggtctccagc tcaccagcga tgcttccaag
     1741 gaggcgccga tcaaggtccg ctccatgggt tgaccttgtc gaaaccccgg ctcacaacgg
     1801 tctccacagg tctcaaccac cgtggatcag cgttcttctg accgtagtga acaccatttc
     1861 tatccggctc aacggacgat ccggaacagc ccggcccaat cgcggccgtc cagcggcaga
     1921 agtctctaga accggcccat actggccgct gccgggcagc tgaaactcgc cctcgccgcc
     1981 cagccgacct gattgatgta gcgcaccacg atcgtcatcg cctgcacgat gttgttctcg
     2041 gcgtacggca cgtcgtgccg gcggcagtgc tcgcgcacca ggcgccgggc cttggccaag
     2101 tgcagccgcg gcatgctcgg gaacaggtga tgctcgatct ggtagttcag cccgcccatc
     2161 agcatggtca tccaccagcc gcccgacacg ttgcgcgaag tgagcacctg cttgtcgaga
     2221 aagtccagct tcgcgccatg cggcacgatc ggcatgccga tgtggttggg cgcgaagctc
     2281 agccccatgt acgtgccgaa cacggccacc tgcacgccca gaaacgcgaa cgcgaggccg
     2341 atcggcaaca gccagaacac cacgcccagg tacagcgaga accgcagcgc gagccacgac
     2401 aactcctgtg cgcggccctc cagcttgccg cgacggaaca gccgtccgat ggacgcctgg
     2461 tgcagatcga ggccgagcag cggcagcagc ggaaagaaca accagccctg gcggcggttc
     2521 agccagcccc ggaagccggt ggcgaccgca gcatcgtcct cggtgaaggc gacccaaccg
     2581 ggggcgatgt cggggtcgcg accgacctgg ttggggttgc cgtggtgtcg attgtgtttg
     2641 gtcatccacc aggcgtagct gatgccgacg aagaaggtgg cgacgacccg tcccagccgg
     2701 tcgttggcgc ggccggaggt gaacacctga cggtgggacg cctcgtgccc gacgaaggcg
     2761 aactgggtga agatcacccc cagtgccgcg gcgatcagca gttggaacca cgagtcgccg
     2821 agcagcgcca tgccggtgaa ggccccggcc agcgcgacga ccaacccgcc gagcagcgcg
     2881 aagtagaacc agcggtggcg gcggttgagg ccctgctcgc gcacccgggc agccagttcg
     2941 gtgaagctgt tggtgcgccg ttcccattcg acccggacgg cctcgcctcg ggctgagcgt
     3001 tgacgcgggg gcgccgggac atcggtggcc gacatcaagt ctcttttctg agcgttcatt
     3061 gcaagagacc cgagcggcga cttcagaacg attggcccaa ccctacggga tcgtggtgaa
     3121 cgcaggaatg ctgagaccca gatccaaccc gagcccgcga ccacgagggc gatcggacct
     3181 ggggacgatc ggacctgagc cccggcgccc gggggccgat cgggccttgg ggcgatctga
     3241 cctggggcga tctgacctgg ggacgatcgg acctgagccc ctcggcgcgg ggctggcnnn
     3301 nnnnnnnnnn nnnnnnnnnn nnnnnnnnnn nnnnnnnnnn nnnnnnnnnn nnnnnnnnnn
     3361 nnnnnnnnnn nnnnnnnnnn nnnnnnnnnn nnnnnnntgg ggcgatctga cctggggacg
     3421 atcggacctg agcccctcgg cgcggggctg gcccgggcgc atgccccaga gttcccgctc
     3481 gggattcgtc cggaaccgat ggcgacgacc ggagcgcgcc ggtccggccc ggctgacgcg
     3541 gttatgggag tgctgatcat tgcgcgatcc gggtcgagcc agtcgacatt caccgaggtc
     3601 tcgacaagct cggccggcga tggatgggtt gcctcctcgg ctgttccggc tccatccact
     3661 ccctagcggg cttgggcgaa gaaggtggtt ggccgcttga ccaggccggc ccgggtcggc
     3721 tcgcaatagc gggtgggttt tggtacagca gtcggtgcca aaacccaccc gctccgttgg
     3781 tgggcgaccg agcaccccca ggcaccttgc ccaatccgcc ggttgagacc gtcgaagacc
     3841 cgacccagga gggtctcaac aggctcgacc agcgaatcct cacgcgatcc gccggttgag
     3901 gccgtcgaga acccgaccca ggagggtctc aacaggctcg accagcggat cctcacgcga
     3961 tccgttggtt gagactgtca aaaccccggt ccatgagggt ctggacaagc taaagacgga
     4021 gatggtcaca ccgtcaggcg atcctcggtt cgaaccatcg cgagaaggcc gcgttcgacg
     4081 gcgtccagcc atggcaccac atgcgcgcga gcggcggccg tgttggggta gcgggcccgc
     4141 aggtgcagcc cggtgtcgtt gacgatgaac cacaccatca cgccgtcgtc gtggatcacg
     4201 gccgagacgt actgcacctg cgcgtccacg ggcagttcgg gcagccggcg cgtgtcgagc
     4261 caggacaggg cgaacatgcc cggtgccgtc ggcatgccgc cgtagggggc gaagatcgga
     4321 gcgagcgggt acgagccgag agcgaccgct tccttgaccg cactcatacc cgctgccggg
     4381 tcggggtcgg cgcactcgag caccgagttg gtgatgaacc agccgaccga ctcgcgccag
     4441 cgaacctcgt cacggctgtg caccgggaac aggacgcgca gcggcgcgtc ggccacgtcg
     4501 cgggtggccg tcacgatcac cgacaacgcc aaggcgatgg aacggacgcc ctgctgcgcg
     4561 gccagcgcac cgaaccgggc agtctgcccg gcgtccagca ggtcgcgcac ctcgaccagg
     4621 ctgtcgccct cgaaggccgg gttgcccagg ggcagcggaa accgtggcat cacaccgtct
     4681 tcggcagcca gcaggccggc ccagcgctca tgcacctccg caggcgctgg gggacgagcc
     4741 tccagcaccg cgctgtgctc ggcgaatgcc ggcaccggtg ccagcccgac acccggggga
     4801 cggccggcgt gcaggtcgtc caggcaggtg cacaggtccc gcaacacgat caggaacgac
     4861 cacatgtcga cgtgcgaatg gtccgagccg acgatcacca tcggacgtgc gtcggcctgg
     4921 tcggcgtcgg gctgcatcac gaccagtagg tgcgaaggtt gctcgaacgg tgcgcaggcc
     4981 gcatcgaaca ggcgccggag cacctcgcgc gggtcgacgc cgtccgctat cgggtgctcg
     5041 cgccactccc ccggcaccgc ctcgaactcg tgcagcacca cccggccctc gccgtccagg
     5101 ccgaagccgg tgcgaagggt gccgtgccgt tgcacgacgg cccaccacgc ctgctgcagg
     5161 tgcgcgggcg tggtgccggc gggcacccgg gcggcgatgg ccatccacga tccggggcgt
     5221 tggccctgcc cgacgtgccg accctgatcg aacgaaaccg gcagggggcg acccatggcg
     5281 tcgctgaggc gcaccccata gctcatcatt cggcccggtg gcagcaccat ccgcgagacg
     5341 ttcgtcagcc gcatgccgcc ttaccctaga agccgaaaac gtcgaaccgg ttacggaggt
     5401 cgccttgccc gtgctgagcg ttcgcgatgc gttgctcgac tacgacgtga ccggcgagag
     5461 cgggccgatc gtggtgcaac tgcacggcct gacctcgtgc cgcgagcgtg acaccgcctt
     5521 gggcttgaac ctggcccggg ctctgggtgg gcaccgcatc gtccgctacg acgcgcgcgg
     5581 ccatggtcgc tcctccggca ccgcggacga ggcgtcctac agttggccgg cgttggcggg
     5641 tgacctgctc gccctgctcg acaccctgtc accggacgcc cccgtgcacg gggtcggcac
     5701 ctcgatgggc tcggccacgc tgctgcatgc cgccgtccgg gcgccgaagc ggttcgccag
     5761 cctgaccctg gcggtgccgc ccaccgcgtg ggagacccgc cgcgccaagg cggaggccta
     5821 ccgggcgaac gccgatctgg tgcagcgcga gggcgtccag gcgttcgtcg acctcggcgg
     5881 cgaaac
//
LOCUS       DAOSUG010000145         5873 bp    DNA     linear   ENV 10-JUN-2024
DEFINITION  MAG TPA_asm: Micropruina sp. isolate AMR_MDS_2195
            NODE_6281_length_5873_cov_4.461499, whole genome shotgun sequence.
ACCESSION   DAOSUG010000145 DAOSUG010000000
VERSION     DAOSUG010000145.1
DBLINK      BioProject: PRJNA925477
            BioSample: SAMN32864262
            Sequence Read Archive: SRR4236673
KEYWORDS    WGS; ENV; Metagenome Assembled Genome; MAG; Third Party Data; TPA;
            TPA:assembly.
SOURCE      Micropruina sp. (wastewater metagenome)
  ORGANISM  Micropruina sp.
            Bacteria; Bacillati; Actinomycetota; Actinomycetes;
            Propionibacteriales; Nocardioidaceae; Micropruina.
REFERENCE   1  (bases 1 to 5873)
  AUTHORS   Abdulkadir,N., Saraiva,J.P., Zhang,J., Stolte,S., Gillor,O.,
            Harms,H. and Rocha,U.
  TITLE     Genome-centric analyses of 165 metagenomes show that mobile genetic
            elements are crucial for the transmission of antimicrobial
            resistance genes to pathogens in activated sludge and wastewater
  JOURNAL   Microbiol Spectr 12 (3), e0291823 (2024)
   PUBMED   38289113
REFERENCE   2  (bases 1 to 5873)
  AUTHORS   Abdulkadir,N.
  TITLE     Direct Submission
  JOURNAL   Submitted (24-MAR-2023) Environmental Microbiology, Helmholtz
            Center for Environmental Research -UFZ, Permoserstr 15, Leipzig
            04318, Germany
COMMENT     The annotation was added by the NCBI Prokaryotic Genome Annotation
            Pipeline (PGAP). Information about PGAP can be found here:
            https://www.ncbi.nlm.nih.gov/genome/annotation_prok/
            
            ##Genome-Assembly-Data-START##
            Assembly Method        :: SPAdes v. 3.11.1
            Genome Representation  :: Full
            Expected Final Version :: Yes
            Genome Coverage        :: Not Applicable
            Sequencing Technology  :: Illumina MiSeq
            ##Genome-Assembly-Data-END##
            
            ##Genome-Annotation-Data-START##
            Annotation Provider               :: NCBI
            Annotation Date                   :: 05/04/2023 04:57:10
            Annotation Pipeline               :: NCBI Prokaryotic Genome
                                                 Annotation Pipeline (PGAP)
            Annotation Method                 :: Best-placed reference protein
                                                 set; GeneMarkS-2+
            Annotation Software revision      :: 6.5
            Features Annotated                :: Gene; CDS; rRNA; tRNA; ncRNA
            Genes (total)                     :: 2,776
            CDSs (total)                      :: 2,750
            Genes (coding)                    :: 2,714
            CDSs (with protein)               :: 2,714
            Genes (RNA)                       :: 26
            tRNAs                             :: 24
            ncRNAs                            :: 2
            Pseudo Genes (total)              :: 36
            CDSs (without protein)            :: 36
            Pseudo Genes (ambiguous residues) :: 0 of 36
            Pseudo Genes (frameshifted)       :: 2 of 36
            Pseudo Genes (incomplete)         :: 32 of 36
            Pseudo Genes (internal stop)      :: 2 of 36
            ##Genome-Annotation-Data-END##
FEATURES             Location/Qualifiers
     source          1..5873
                     /organism="Micropruina sp."
                     /mol_type="genomic DNA"
                     /submitter_seqid="NODE_6281_length_5873_cov_4.461499"
                     /isolate="AMR_MDS_2195"
                     /isolation_source="Activated sludge of municipal
                     wastewater treatment plants"
                     /db_xref="taxon:2737536"
                     /environmental_sample
                     /geo_loc_name="Canada"
                     /lat_lon="49.25 N 123.1 W"
                     /collection_date="2015-06-16"
                     /metagenome_source="wastewater metagenome"
                     /note="metagenomic"
     gene            145..1386
                     /locus_tag="PLF56_06125"
     CDS             145..1386
                     /locus_tag="PLF56_06125"
                     /EC_number="1.2.1.41"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_013864465.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_function: GO:0004350 - glutamate-5-semialdehyde
                     dehydrogenase activity [Evidence IEA];
                     GO_function: GO:0016620 - oxidoreductase activity, acting
                     on the aldehyde or oxo group of donors, NAD or NADP as
                     acceptor [Evidence IEA];
                     GO_process: GO:0006561 - proline biosynthetic process
                     [Evidence IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="glutamate-5-semialdehyde dehydrogenase"
                     /protein_id="HOQ53176.1"
                     /translation="MNFTDQAHAAKQAAISLATLNRATKDAALHAMADALAEAEPVVL
                     EANLRDVERARAAGTPDAMIDRLQLTPKRVAGMVAGLRDLAGLPDPVGDVIRGWTNAN
                     GVQVRQVRVPFGVVGIIYEARPNVTADAAGICLKSGNAALLRGSSSAIESNRAVVVAL
                     RAGLQAAGLPVDAVQLVEGGREVTAELMAARGLVDVLIPRGGAGLINAVVQGSTVPVI
                     ETGTGNCHIFVDATADQAMAIDILLNAKVQRPSVCNAAETLLVHRAIAEEFLPKTLEA
                     LAAEGVTVHGTQSVQGYSEAVVPATSDEFDGEYLSLDLAAAVVDSVNAAIDHIRAHTT
                     GHSETIITNDRSSADRFVAQVDAAAVLVNASSRFVDGGEFGFGAEIGISTQKLHARGP
                     MGLAEMTSSKYVLTGDGQVRP"
     gene            1625..1756
                     /locus_tag="PLF56_06130"
     CDS             1625..1756
                     /locus_tag="PLF56_06130"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_002548582.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="hypothetical protein"
                     /protein_id="HOQ53177.1"
                     /translation="MGILLETATHDAYSPYLVGAIVLAVLMIALRVVFGVGKSRPHS"
     gene            1756..2388
                     /gene="nadD"
                     /locus_tag="PLF56_06135"
     CDS             1756..2388
                     /gene="nadD"
                     /locus_tag="PLF56_06135"
                     /EC_number="2.7.7.18"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_002541320.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_function: GO:0016779 - nucleotidyltransferase activity
                     [Evidence IEA];
                     GO_process: GO:0009435 - NAD biosynthetic process
                     [Evidence IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="nicotinate-nucleotide adenylyltransferase"
                     /protein_id="HOQ53178.1"
                     /translation="MTNPGIVWTGDGSRRRRLGVMGGTFDPIHHGHLVAASEVGTKFK
                     LDEVVFVPTGQPWQKADRQVSLAEDRYLMTTIATASNPRFSVSRVDIERPGSTYTLDT
                     LRDLRNHYGADVDLFFITGADALSQILTWHGVLELFELAHFVGVTRPGTTLGEHDIAH
                     LPPDKVTVIEVPALAISSTDCRKRVKERLPIWYLVPDGIVQYIAKRGLYR"
     gene            2421..2822
                     /gene="rsfS"
                     /locus_tag="PLF56_06140"
     CDS             2421..2822
                     /gene="rsfS"
                     /locus_tag="PLF56_06140"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_013864462.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_function: GO:0043022 - ribosome binding [Evidence IEA];
                     GO_process: GO:0017148 - negative regulation of
                     translation [Evidence IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="ribosome silencing factor"
                     /protein_id="HOQ53179.1"
                     /translation="MTATDHAIALTRVAAQAASDKLGSDLVAFDVSEPLAITDVFLIV
                     SASNERQVGALVDAVEEALLRQGAKPARREGHGENRWVLLDFLDLVVHVMHNEERALY
                     TLERLWSDCPRIDLQLDTRMPDGVATLDSPA"
     gene            2824..3438
                     /locus_tag="PLF56_06145"
     CDS             2824..3438
                     /locus_tag="PLF56_06145"
                     /EC_number="3.1.3.-"
                     /inference="COORDINATES: protein motif:HMM:NF012521.2"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="histidine phosphatase family protein"
                     /protein_id="HOQ53180.1"
                     /translation="MTAARVVLWRHGETDWNATGRYQGQADVQLNERGVQQARAAAPM
                     IAALGPGRLVSSDLGRARRTAAELAGLTGLTLEVQPRLQEINVGDWAGLTNEQVFAAH
                     PDFAEALRTGRDARRGGTGETGAEAGARVAEALLDLAESTPDGQTVVAVGHGFAMRVA
                     TVFLLGLDYAHNLTLGGLWNASWTVLQPAGETWRLLSYNNVAQG"
     gene            complement(3458..5869)
                     /locus_tag="PLF56_06150"
     CDS             complement(3458..5869)
                     /locus_tag="PLF56_06150"
                     /inference="COORDINATES: protein motif:HMM:NF013491.2"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_function: GO:0005524 - ATP binding [Evidence IEA];
                     GO_function: GO:0016301 - kinase activity [Evidence IEA];
                     GO_process: GO:0016310 - phosphorylation [Evidence IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="PEP/pyruvate-binding domain-containing protein"
                     /protein_id="HOQ53181.1"
                     /translation="MGEPAVAVRSSATTEDLPDASFAGQQDTFLNVQGADALLAATRR
                     CWASLWGERALAYRAQHAADSAPRIAVVVQRLVVADVAGVMFTADPANGRRDQTVITA
                     AWGLGEAVVGGLVEPDEFVLDRAAPDRDPGAVLESTPEWRVGRRIAAKRVRTVTTAGG
                     SEAQPTPPDLVEAPTLTDAQVRELAALGERVQAHFGRPQDIEWVLADGTFQLVQARPI
                     TALPEPTGDIPTEWPIPRADCLYFRASIVEQLPDPLTPLFADLIRPAVPAGLNALMTE
                     LSPRTAGLDVDFPTISGYAYYAYAKKALNRMWGITPSALRMLSRRNFVISRLRDRALP
                     AYRSAVASWSGQDPARLSATALLTATRELLEAGCRYYTNVQMVIPVAATTELSWTGLY
                     DAVLRGSDEAQASDFLLGFDSTPILAEKSLYRVADWCRGVPGLAEALNGLTAVPDEAP
                     SGVSPSDWTRFRDRFGAHLSEYGHTLYNLDFANPVPADDPAPVLEALKHALSGRAADP
                     VARQRALAERRSRITRELMDRLDPVRRKLARGSLAAAQHWAPIREDALAAMGLAWPTM
                     RRLLAELGRRLVRAGAIAQPDQVYWLTSAEVDAAASALDAGTTTLRDQVALVEQRRMT
                     WRGQALATPPQYLPEAGWVTLMDRFMPAHTGDQSGPVLKGTGGSGGRVTARARVLAGA
                     EDFAAFQPGEILVASITTPAYTPLFALAAGVVTDIGGVLSHGSIVAREYGIPAVLGTG
                     VATRRISTGDVISIDGGTGTVRLDGEPQDSSPAGHAVPAWAWVTGVAAASAGVVALVA
                     RRRRA"
BASE COUNT          912 a         2008 c         2091 g          862 t
ORIGIN      
        1 gtgaagatat accgaatcta ccgccagatt ctcgatgaaa cgatgcaaac ggcatagtcg
       61 agaaatctag cggtagattc ggtatatcaa cgtctgaaga gatatccgaa gcgagtggct
      121 gcaggctggt tgtattctgc ggccgtgaac ttcaccgacc aggcccacgc cgccaagcag
      181 gcggccatct cgcttgccac gctcaaccgg gccaccaagg acgccgccct gcacgcgatg
      241 gccgacgcgt tggccgaggc cgagcccgtc gttctcgagg ccaacctgcg cgacgtcgaa
      301 cgggcccgtg cagccggtac gccggatgcc atgatcgacc ggctgcagct caccccgaag
      361 cgcgtggccg gcatggtcgc cggcctgcgc gacctggccg ggttgcccga cccggtcggt
      421 gacgtgatcc gcggctggac caacgccaac ggtgtgcagg ttcgacaggt gcgggtgccg
      481 ttcggtgtcg tcggcatcat ctacgaggca cgtccgaacg tcaccgccga cgcggccggt
      541 atctgcctga agtcgggtaa tgcggcactg ctgcgcggct cgtcctcggc gatcgaatcg
      601 aatcgtgcgg tcgtcgttgc cctgcgcgcc gggttgcagg cggccggatt gccggtcgat
      661 gcggtccagt tggtcgaggg cggccgcgag gtgacggccg aactgatggc cgcgcggggc
      721 ctggtcgacg tgctgatccc gcgcggcggc gccggtctga tcaacgcggt cgtccagggc
      781 agcacggtgc cggtcatcga gaccggcacc ggcaactgcc acatcttcgt ggacgccacg
      841 gccgaccagg cgatggccat cgacattctg ctgaacgcca aggtgcaacg gcccagcgtc
      901 tgcaacgccg ccgagacgct gctggtgcat cgggcgatcg cagaagagtt cctgccgaag
      961 acgctcgaag cgctggccgc cgagggcgtc accgtgcacg gcacccagtc ggtgcagggc
     1021 tactcagagg ccgtggtgcc cgccacgtcc gacgagttcg acggcgagta tctctcgctc
     1081 gacctggccg ccgccgtggt cgactcggtc aacgcggcca tcgaccacat tcgcgcccac
     1141 accacgggcc actccgagac catcatcacc aacgaccgca gcagcgccga ccggttcgtc
     1201 gcccaggtgg acgcggccgc cgtgctggtc aacgcatcgt cccggttcgt ggacgggggc
     1261 gagttcggtt tcggcgccga gatcggtata tcgacccaga agctgcacgc ccgcgggccg
     1321 atgggcctgg ccgaaatgac ctcgtccaag tacgtgctga ccggggacgg gcaggtgcgt
     1381 ccctagggtg tgtctcctca ccctgggcgt tccgcgagga cgtgcccagt gcgtctatca
     1441 cagggcggaa gacgagtcga tagcgggtcc tctcgcggaa gatgacaacg cagtgagcag
     1501 gcgtactggg tgcgccgcag cgcggcaagg gtgaggagac accccctagc ggacggggga
     1561 acatccacga cggcccgacc catggtcgtg gcactggccg ggagctgccc actatcatcc
     1621 gcgcatgggc attcttcttg agacggccac gcatgacgcg tactcgcctt acctggtggg
     1681 cgccatcgtg ttggccgtgc tgatgatcgc gttgcgggtc gtgttcggcg tcggcaagag
     1741 ccgtccgcat agctgatgac gaatccgggc atcgtctgga ccggtgacgg ctccaggcgg
     1801 cgacgcctcg gggtgatggg tggcacgttc gacccgattc accacggcca cctggtggcg
     1861 gccagtgagg tgggcaccaa gttcaagctg gacgaggtcg tgttcgtgcc gaccggccag
     1921 ccctggcaga aggccgatcg gcaggtgtcg ctggccgagg accgttacct gatgaccacg
     1981 atcgcgacgg cgtccaaccc gcgcttctcg gtcagccggg tcgacatcga gcgtcccggc
     2041 agcacttaca ccctcgacac cctgcgcgac ctgcgtaacc actacggcgc cgatgtggac
     2101 ttgttcttca tcaccggagc ggacgcgctc agccagatcc tcacctggca cggcgtgctg
     2161 gagctgttcg aactggcaca tttcgtgggg gtgacccggc ccggcaccac gctcggtgag
     2221 cacgacatcg cccacctgcc gcccgacaag gtgaccgtga tcgaggtgcc cgcgctggcg
     2281 atctcgtcca cggactgccg caagcgggtc aaggagcggc tgccgatctg gtatctggtg
     2341 ccggacggta tcgtccagta catcgccaag cgcggcctct accgctgagc cgcccgatcc
     2401 accgcaccac tgggagctcg atgactgcaa cagaccacgc tatcgccctc acgcgcgtcg
     2461 ctgcccaagc ggcgtccgac aagctgggaa gcgatctggt ggccttcgat gtgtccgagc
     2521 cgttggcgat caccgacgtg ttcctgatcg tcagcgcgtc caacgagcgg caggtgggcg
     2581 ccttggtcga cgccgtcgaa gaggccctgc tgaggcaggg cgccaagcct gcccggcgcg
     2641 agggccacgg tgagaaccgg tgggtgctgc tcgactttct cgacctggtg gtccacgtca
     2701 tgcacaacga ggagcgggcg ctctacacgc tggagcggct gtggagcgac tgtccgcgca
     2761 tcgacctgca gctcgacacc cgcatgccgg acggggtcgc gacgctcgat tcccccgcct
     2821 gaggtgacag cagcacgggt cgtgctctgg cggcacggcg aaacggactg gaacgccacc
     2881 gggcgctatc aggggcaggc ggacgtccag ctgaacgaac gcggggtaca gcaggccagg
     2941 gcggccgcgc cgatgatcgc cgctttgggt ccgggccgac tggtgtcgtc cgatctcggc
     3001 cgggcgcgac gcaccgccgc cgagttggcg gggctgaccg ggctgaccct cgaggtgcaa
     3061 ccccggctgc aggagatcaa cgtcggcgac tgggcggggc tgaccaacga gcaggtgttc
     3121 gcggcccatc ccgacttcgc cgaagcgttg cgcaccggac gggacgcgcg acgcggcggc
     3181 accggagaaa cgggcgccga ggcgggcgcg agggtggccg aggcgttgct cgacctcgct
     3241 gagagcaccc cggacggaca gaccgtggtg gccgtcgggc acggcttcgc aatgcgggtg
     3301 gccacggtgt ttctgctcgg cctcgactac gcgcacaacc tgaccctggg cgggctgtgg
     3361 aacgcctcgt ggacggtgct gcagcccgcg ggcgagacct ggcggctgct gtcgtacaac
     3421 aacgtggcgc agggctgacg gggtcggcga actcggttca cgcccgccgc cgccgggcca
     3481 ccagggcgac cacacccgcg gacgccgctg caacccccgt cacccaggcc caggccggaa
     3541 cggcgtggcc ggccggtgag gagtcctgcg gttccccgtc caaccggacg gtgcccgtgc
     3601 ccccgtcgat gctgatcacg tcgccggtcg agattcgcct ggttgccacg cccgtgccca
     3661 gcacggccgg aatgccgtac tcgcgggcca cgatcgagcc atggctgagc accccgccga
     3721 tatcggtgac cacgcctgcg gcaagggcga acagtggcgt gtaggcgggc gtggtgatcg
     3781 aggccaccag aatctcgccg ggctggaagg ccgcgaagtc ctcggccccg gcgagcacgc
     3841 gggcgcgcgc cgtgacccgt ccgcccgaac cgccggtgcc cttgagtacc ggtccgctct
     3901 ggtcgccggt gtgggcgggc atgaagcggt ccatcagcgt gacccagccg gcctccggca
     3961 agtactgcgg gggagtcgcc agcgcctgac cgcgccatgt catgcgacgt tgctcgacca
     4021 gcgccacctg atcgcgcagc gtggtggtgc cggcatcgag ggcggacgct gcggcgtcca
     4081 cttcggccga ggtcagccag tacacctggt cgggctgcgc gatcgcgccg gcccggacga
     4141 ggcgccgccc cagttcggcg agcaggcgcc gcatggtcgg ccaggccagc cccatcgcag
     4201 ccagcgcgtc ctcacggatc ggcgcccagt gctgagcggc ggccaggctg ccacgggcca
     4261 gcttgcgacg caccggatcg agccggtcca tcaactcccg ggtgatccgg ctgcgccgct
     4321 cggccagggc gcgctgccgt gcgaccgggt cggcggcccg tccggacagt gcgtgcttga
     4381 gcgcctccag caccggggcc gggtcgtcgg cgggcaccgg gttggcgaag tcgaggttgt
     4441 acagggtgtg cccgtactcg ctcaggtgcg cgccgaaccg gtcgcgaaac cgcgtccagt
     4501 cggacgggct cacgccggac ggtgcctcgt ccggcaccgc cgtcaagccg ttcaacgcct
     4561 ccgcaagccc cggcacgccg cggcaccagt cggcgacgcg gtacagcgac ttctcggcga
     4621 ggatcggggt ggagtcgaac ccgagcagaa aatcggacgc ctgagcctcg tccgaaccgc
     4681 gcagcaccgc gtcgtacagc cccgtccagc tgagttcggt ggtggcggcg accggaatca
     4741 ccatctgcac gttggtgtag taccggcagc cggcctcgag cagctcgcgg gtcgcggtga
     4801 gcagcgcggt ggcgctgagc cgcgcgggat cctgccccga ccacgaggcg acggcgcttc
     4861 ggtaggcagg cagcgcccgg tcgcgcagcc gcgagatcac gaagtttctc cggctcagca
     4921 tgcgcaacgc ggacggtgtg atgccccaca tccgattcag cgccttcttg gcgtaggcgt
     4981 agtaggcgta cccgctgatg gtgggaaaat cgacgtcgag gccggccgtc ctcgggctca
     5041 actcggtcat cagggcgttg agcccggccg ggacggcggg ccggatcagg tcggcgaaca
     5101 ggggagtgag cgggtcgggc agctgctcga cgatgctggc ccgaaagtac aggcagtcgg
     5161 cgcgcgggat cggccactcg gtcggtatgt cgccggtcgg ttcgggtagc gccgtgatcg
     5221 gtcgggcctg caccagctgg aacgtgccgt cggcgagcac ccactcgatg tcttgcggtc
     5281 gtccgaaatg cgcctggacg cgctcaccga gcgcggccag ttcgcgcacc tgggcatcgg
     5341 tcagcgtggg cgcctcgacc aggtcgggtg gggtcggctg cgcttcggac ccgccggcgg
     5401 tcgtgaccgt gcgcacccgc ttggccgcaa tgcgccggcc cacgcgccat tcgggggtgg
     5461 attcgagcac tgcaccgggg tcccgatcag gagccgcacg gtcgagcacg aactcgtcgg
     5521 gttcgaccag gccgccgacc accgcttcgc cgaggcccca ggcggcggtg atcacggtct
     5581 ggtcgcggcg tccgttggcc gggtcggcag tgaacatcac cccggcgacg tcggccacca
     5641 ccagccgctg caccaccacc gcgatgcgag gcgcagaatc ggccgcatgc tgagcccgat
     5701 aggcgagggc ccgctcgccc cacagtgagg cccagcagcg ccgcgtcgcg gcgagcagcg
     5761 cgtccgcgcc ctgcacgttg aggaaggtgt cctgctggcc ggcgaagctc gcatcgggca
     5821 ggtcttcggt ggtcgcggac gagcgcacgg cgacggccgg ctcgcccagg gcc
//
LOCUS       DAOSUG010000146         5839 bp    DNA     linear   ENV 10-JUN-2024
DEFINITION  MAG TPA_asm: Micropruina sp. isolate AMR_MDS_2195
            NODE_6317_length_5839_cov_4.151625, whole genome shotgun sequence.
ACCESSION   DAOSUG010000146 DAOSUG010000000
VERSION     DAOSUG010000146.1
DBLINK      BioProject: PRJNA925477
            BioSample: SAMN32864262
            Sequence Read Archive: SRR4236673
KEYWORDS    WGS; ENV; Metagenome Assembled Genome; MAG; Third Party Data; TPA;
            TPA:assembly.
SOURCE      Micropruina sp. (wastewater metagenome)
  ORGANISM  Micropruina sp.
            Bacteria; Bacillati; Actinomycetota; Actinomycetes;
            Propionibacteriales; Nocardioidaceae; Micropruina.
REFERENCE   1  (bases 1 to 5839)
  AUTHORS   Abdulkadir,N., Saraiva,J.P., Zhang,J., Stolte,S., Gillor,O.,
            Harms,H. and Rocha,U.
  TITLE     Genome-centric analyses of 165 metagenomes show that mobile genetic
            elements are crucial for the transmission of antimicrobial
            resistance genes to pathogens in activated sludge and wastewater
  JOURNAL   Microbiol Spectr 12 (3), e0291823 (2024)
   PUBMED   38289113
REFERENCE   2  (bases 1 to 5839)
  AUTHORS   Abdulkadir,N.
  TITLE     Direct Submission
  JOURNAL   Submitted (24-MAR-2023) Environmental Microbiology, Helmholtz
            Center for Environmental Research -UFZ, Permoserstr 15, Leipzig
            04318, Germany
COMMENT     The annotation was added by the NCBI Prokaryotic Genome Annotation
            Pipeline (PGAP). Information about PGAP can be found here:
            https://www.ncbi.nlm.nih.gov/genome/annotation_prok/
            
            ##Genome-Assembly-Data-START##
            Assembly Method        :: SPAdes v. 3.11.1
            Genome Representation  :: Full
            Expected Final Version :: Yes
            Genome Coverage        :: Not Applicable
            Sequencing Technology  :: Illumina MiSeq
            ##Genome-Assembly-Data-END##
            
            ##Genome-Annotation-Data-START##
            Annotation Provider               :: NCBI
            Annotation Date                   :: 05/04/2023 04:57:10
            Annotation Pipeline               :: NCBI Prokaryotic Genome
                                                 Annotation Pipeline (PGAP)
            Annotation Method                 :: Best-placed reference protein
                                                 set; GeneMarkS-2+
            Annotation Software revision      :: 6.5
            Features Annotated                :: Gene; CDS; rRNA; tRNA; ncRNA
            Genes (total)                     :: 2,776
            CDSs (total)                      :: 2,750
            Genes (coding)                    :: 2,714
            CDSs (with protein)               :: 2,714
            Genes (RNA)                       :: 26
            tRNAs                             :: 24
            ncRNAs                            :: 2
            Pseudo Genes (total)              :: 36
            CDSs (without protein)            :: 36
            Pseudo Genes (ambiguous residues) :: 0 of 36
            Pseudo Genes (frameshifted)       :: 2 of 36
            Pseudo Genes (incomplete)         :: 32 of 36
            Pseudo Genes (internal stop)      :: 2 of 36
            ##Genome-Annotation-Data-END##
FEATURES             Location/Qualifiers
     source          1..5839
                     /organism="Micropruina sp."
                     /mol_type="genomic DNA"
                     /submitter_seqid="NODE_6317_length_5839_cov_4.151625"
                     /isolate="AMR_MDS_2195"
                     /isolation_source="Activated sludge of municipal
                     wastewater treatment plants"
                     /db_xref="taxon:2737536"
                     /environmental_sample
                     /geo_loc_name="Canada"
                     /lat_lon="49.25 N 123.1 W"
                     /collection_date="2015-06-16"
                     /metagenome_source="wastewater metagenome"
                     /note="metagenomic"
     gene            <1..808
                     /locus_tag="PLF56_06155"
     CDS             <1..808
                     /locus_tag="PLF56_06155"
                     /inference="COORDINATES: protein motif:HMM:NF039718.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=2
                     /transl_table=11
                     /product="DUF6297 family protein"
                     /protein_id="HOQ53182.1"
                     /translation="YALDFGLVRDIVVERAAVERGHVKPTSGRGTGLQALFWRDLQRL
                     ARFPRPLVPLAASVVAPYALDALGMTTLNPFISGLILVVVLVPFLSMLRVLTRTGGLA
                     RMFPFRTSQVRTVAMVVPLILALIWQAATIPAFVGITSGGAERSVLDGALVALITGIA
                     GWLGAVRWVTAKKVDFNSPMVATESGAVPPTLILNLFRGLDMVALVTAPVMLGQSPMW
                     SFALAFVAFIFLRGTFNTDEMKAQQEQLNREKEAAKKASGEKIKIQRPTR"
     gene            1234..>5839
                     /locus_tag="PLF56_06160"
     CDS             1234..>5839
                     /locus_tag="PLF56_06160"
                     /inference="COORDINATES: ab initio
                     prediction:GeneMarkS-2+"
                     /note="Derived by automated computational analysis using
                     gene prediction method: GeneMarkS-2+."
                     /codon_start=1
                     /transl_table=11
                     /product="hypothetical protein"
                     /protein_id="HOQ53183.1"
                     /translation="MEDLSQRSGSVRVLVNPDGTSEQEAHAAPVWVKDADGKWVDVDY
                     SLVPRTGGGFAPKASPSSVVIDAGGSGEFARVVLPGGGSTIWSWPEALPVPAVQGATA
                     TYGVADGVDLLVTATGLGVSSRIRINSADAVVPEFTVQVRTVGVELKQTGEGQLFFTD
                     GEDRAGQTSTLTAWDGRLDSFGDPLEVVPVEASLQETAAKGERTDQELTLTTPQVLVD
                     DPEVVYPIVIDPDLTPLVASQDTWVRLGTSTIDTLSYRVLVGRVASHANDNQAFGFMR
                     WPSGQITGRKVLKANIHLFQYRAGTCAARQMNMHPLVGVWDEATTVYANKPEIVTTSG
                     TSSSLTKNVGGDGCAANGFIDGDLTKMVQAWANGPANGGFANYGVRFNVPEASKSDVS
                     YERGFCSINYDPTHTSCNSAARVPYLSVTYNSAPQTAATPAVNASRTFDGKLWTSTAT
                     PTFSTSATDAEASKVTYSFEVRTFEGAATVTSSCTTGQVAAGASASCKPVTALTDGAS
                     YVVRARATDEHGLVGDWSAWRALGVDTTAPSEPTVSCSGYTNDSWQASRVGPTTTCSF
                     TATGVADFEWRRTQAGTVEDQPAVIATGGSGTTAAIPVPEVGVVKVEARARNKSGLAS
                     GWKIFTFGIGTAAVTQPMLDDRSTSTFPVQAVAGGGAISAQVQWRYAPDVEGDTTTGW
                     TAATKLQLKSTGAAWTGSLQTSSPLSQTPLLTWTPSKEPGISVPSTVQLRVVFSYPGN
                     VTMPSPMQRVVLIPHAFGGSYPTQDVGAGTLALFTGEYQLSETDVSVPGTGGNLTVGR
                     THGTLTGDLAGPPGVFGPGWTADFAGEGTGAAGYVVTDNTALDGTLILTSPGGESDTY
                     AHSTGTKGALKTGTYKGVGETALNLDKLVLATGGGTGISHTLTLTELDGTITEFQRNT
                     AGIWSTHRTVEPEDNSTVQFVRDSNGLITWILAPAPTGVTCTAATQNKGCRALKFTYT
                     TVNGAARLTKVQYRAWDPKPGADGKPSAAAAMATVDVAGYGYDAQGRLTEAWEPNASG
                     DAGAGRKTQYEYTTINSKTVVSKVSDPGLVPWRFAYDAVGRLATVKRALDPAVGTGDA
                     TWSVAYDVPLSGPGLPELTVNAVAGWGQLAADAPTGATAVFDPDHPLSGSPGEADWAY
                     GSLSYFTQAGRTTNTAVYGAGQWLIDSTRYDAQGNTTWNLSGEGRALALTEPEPATAA
                     DKYATLTVYNTAGTRVEETYSPTRPVVLNNGGIVVARTLTSTVYDDEADTSLTPGRPS
                     SGVPDGGYQLAVEQTTAVTDRILPKADGNTWDTNKTRYRYDPVVAADPSGWLLKVPTR
                     TLTQDGSGWATTLTRYDSNGRIVETRTPGGTAITNGTANDTYSTKTIYYTADTSASVS
                     ACRSKPEWDGNACIVKTGGDPSTGYPIPAKTTTGYSIFGAVTRLEETGSTWSRATITG
                     FDYQGRETSGSTALTDHTTISGTTSYHATTGAVTATTRGGITENFTHDSWGRVLTSTD
                     GTGNTATTSYDTAGRTKTFNDGKGSYTYSYDGTDNLGQVEH"
BASE COUNT          999 a         1828 c         1969 g         1043 t
ORIGIN      
        1 gtatgccctc gactttggct tggtgcgcga catcgtggtg gaacgtgccg cggtcgaacg
       61 tggccatgtg aagccgacgt ccggccgggg caccgggctg caggcgttgt tctggcgtga
      121 cctgcagcgg ctggcccggt tcccgcgtcc gttggtgccg ctggcggcct cggtggttgc
      181 tccttacgcc ctggacgcgt tgggcatgac cacgctcaac ccgttcattt cggggctgat
      241 cctggtcgtg gtgctggtgc cgttcctgtc gatgctgcgg gtgctcaccc gcaccggggg
      301 actggctcgc atgttcccgt tccgcacctc gcaggtgcgc acggtggcga tggtggtgcc
      361 gctgattctg gccctgatct ggcaggcggc cacgattccg gcgttcgtcg gcatcacgtc
      421 cggaggtgcc gagcgcagcg tgttggacgg cgctctggtg gctctgatca cgggtatcgc
      481 gggatggctc ggggccgtcc ggtgggtgac ggcgaagaag gtcgacttca acagcccgat
      541 ggttgccacc gagtcggggg cggtgccgcc gacactgatt ctgaacttgt tccgcggcct
      601 ggacatggtg gccctggtga cggcgccggt gatgttgggc cagtcgccga tgtggtcgtt
      661 cgcgctggcg ttcgtggcgt tcattttctt gcggggcacc ttcaacactg atgagatgaa
      721 ggcccagcag gaacagttga accgtgagaa ggaggccgcg aagaaggcct cgggggagaa
      781 gatcaagatc cagcggccga cccgttagct gcaatcgcgc ccactcgatt gtgctcaggc
      841 aaggaccgag tgcgcatgag gtggtcacgg tttggtcacg gctgaccgcc ggggttgcgg
      901 aactcatctg tggtcgggga tagtttcgga ttcgcccacc ctgtgctggc taatgacggc
      961 cgccgggttt gttgtgagag gcatttcgtg ttgcgcttgt tcgtgtcttc ggctggtttc
     1021 cggctgcctc gctgggtcgt tgttgtgctg gttgctgtgt tgtgtgtggg gttgtcgttc
     1081 ggcagcgagt tgccgtcgcg ggctgcggct cctgtgttgc cggttgagtc gggtgaggag
     1141 gcggtgaggg ctgctgattc tgatggggat ggggtgccgg atcggcctga tttggtgtcg
     1201 gcgggtgtga cggctcgggt tttgggtgtt gcggtggagg atttgtcgca gcggtcgggg
     1261 tcggtgcggg tgttggtgaa tccggatggg acgtcggagc aggaggcgca tgcggcgccg
     1321 gtgtgggtga aggacgcgga cggcaaatgg gtggatgtgg actacagcct ggtcccgcgc
     1381 acggggggcg ggtttgctcc gaaggcgtcg ccgtcgtcgg tggtgattga tgcgggcggg
     1441 tcgggggagt ttgcccgggt ggtgttgccg ggcggcggtt cgacgatctg gtcgtggcct
     1501 gaggctttgc cggtaccggc ggtgcagggt gccaccgcga cttatggggt ggctgatggt
     1561 gtggatttgt tggtgacggc gacgggtttg ggggtgtcgt cccggattcg gatcaattcg
     1621 gctgatgcgg tggtgccgga gttcacggtg caggtgcgca cggtgggtgt ggagttgaag
     1681 cagaccggtg agggtcagtt gttcttcacc gacggtgagg atcgggccgg gcagacgtcg
     1741 acactcacgg cgtgggatgg gcgtttggac tcttttggtg atccgttgga ggtggtgccg
     1801 gtggaggcca gccttcagga gacggccgcc aagggtgagc gcactgatca ggagttgacg
     1861 ctgacgactc ctcaggtgtt ggttgatgat cctgaggttg tgtatccgat tgttattgat
     1921 cctgatttga ctccgttggt ggcgtcgcag gacacgtggg tgcggttggg cacctcgacg
     1981 attgacacgt tgtcgtatcg ggtgttggtg gggcgggtcg cgtcgcatgc gaatgataat
     2041 caggcgtttg gtttcatgcg gtggccgagt gggcagatca cgggccggaa ggttttgaag
     2101 gccaatattc atttgtttca gtatcgggcg ggtacgtgtg cggcgcggca gatgaacatg
     2161 catccgttgg tgggggtgtg ggatgaggcg accacggtgt atgcgaacaa gcccgagatt
     2221 gtgacgacgt cgggcacgtc gtcgtcgttg acgaagaatg tgggtggtga tgggtgcgca
     2281 gcgaacgggt tcattgatgg tgatttgacg aagatggtgc aggcgtgggc gaacgggccg
     2341 gctaatggtg ggttcgcgaa ctatggtgtg cggttcaatg tgccggaggc gagcaagagc
     2401 gatgtgagtt atgagcgcgg gttctgttcg atcaactatg acccgacgca cacctcgtgc
     2461 aactcggccg cacgggtgcc gtacttgtcg gtcacctaca acagcgcccc acagacggcg
     2521 gcaacgcccg cggtgaatgc gtcgcgcacg ttcgacggca aactgtggac ctcgaccgcc
     2581 acgccgacgt tctccacgtc ggcgacggat gcggaagcct cgaaggtcac ctacagcttc
     2641 gaggtgcgta cctttgaggg tgccgccacg gtcacctcca gttgcaccac cggccaggtc
     2701 gctgccgggg cgagcgccag ctgcaaaccc gtcaccgcat tgaccgatgg tgcaagttat
     2761 gtggtgcgcg cccgggccac cgatgagcac ggcctggtcg gcgactggtc agcatggcgt
     2821 gctttgggtg tagacaccac agccccatcg gagccgaccg tttcctgctc cggctacacg
     2881 aacgactcgt ggcaggccag ccgtgtcggc cccacgacga cctgctcgtt cactgctacc
     2941 ggggtggccg attttgaatg gcgccgcacc caggcgggca ccgtggagga ccagccggcg
     3001 gtgatcgcca ccggcggttc cggcaccacg gccgccatcc cggtgcccga ggtgggtgtg
     3061 gtgaaggtcg aagcccgggc gcggaacaag tccggcttgg cgtcgggttg gaagatcttc
     3121 acgttcggta ttggtaccgc tgcggttacc cagccgatgc tggacgacag atccacctcc
     3181 acgttcccgg ttcaagcggt cgctggcggt ggcgcaatct ccgcccaggt tcagtggcgt
     3241 tacgcccccg atgttgaggg tgacaccacc accggctgga ccgcggccac gaaactgcag
     3301 ctcaagtcga ccggcgcagc gtggaccgga tcgttgcaga ccagttcccc gttgtcgcag
     3361 acacccttgt tgacttggac gccgtcgaag gagcccggta tttcggtgcc gtcaacagtc
     3421 cagttgcggg tcgtgttcag ctatccgggc aacgtcacaa tgccttcgcc gatgcagagg
     3481 gtggtgttga ttccgcacgc tttcggtggt tcctacccga cccaagacgt cggcgcaggc
     3541 accctggcgt tgttcaccgg cgagtaccaa ctgtcggaaa ccgatgtgtc ggtgcccggc
     3601 acgggcggga acctgaccgt gggccgcacc cacggcaccc tcaccgggga tctggccggg
     3661 ccgcccggcg tgttcggacc tggttggacc gccgacttcg cgggcgaagg caccggcgcc
     3721 gctggctacg tggtcaccga caacaccgcc ctcgacggca ccctcatcct cacctcacca
     3781 ggcggcgagt ccgacaccta cgcccactcc accggaacca aaggtgcact caaaaccggc
     3841 acctacaagg gagtcggaga gaccgcgctc aacctggaca aactcgtcct ggccaccggt
     3901 ggtggcaccg ggatcagcca caccctcacc ctgaccgaat tggacggcac catcaccgag
     3961 ttccaacgca acaccgccgg gatctggtca acccaccgca cggtcgaacc cgaggacaac
     4021 tcgacggttc agttcgtccg ggacagcaac gggctgatca cctggattct cgcgcccgct
     4081 ccgaccgggg tgacgtgcac cgcagcgacc cagaacaagg gctgccgggc gttgaagttc
     4141 acctacacca ccgtcaatgg ggcggcgcgg ctcaccaagg tgcagtatcg ggcgtgggat
     4201 cccaaaccgg gcgctgatgg gaaaccgtcc gctgcggctg cgatggccac cgttgatgtg
     4261 gccggttacg gttacgacgc ccagggtcgg ctgaccgaag cgtgggaacc caacgcgtcc
     4321 ggtgatgccg gcgcgggccg caaaacccag tacgaataca ccaccatcaa ctccaagacc
     4381 gtcgtgtcga aggtcagcga cccaggcctg gtgccgtggc gcttcgctta cgacgctgtg
     4441 ggccgactcg ccactgtgaa acgtgccctc gaccctgccg tcggaaccgg agacgccacc
     4501 tggagcgtcg cctacgacgt ccccctctcc gggcccggtt tgcccgagct caccgtcaac
     4561 gccgtagctg gctggggaca actcgcagcc gacgcaccca ccggcgccac ggcagtcttc
     4621 gaccccgacc acccactgtc gggctctccc ggcgaagccg attgggccta cgggagtctg
     4681 tcgtacttca cccaagcagg ccggaccacc aacaccgccg tctatggcgc cggccaatgg
     4741 ctgatcgact ccacccgtta tgacgcacaa ggcaacacca cctggaacct gtccggcgag
     4801 ggccgagccc tcgcactcac cgaacctgag ccggccacgg ccgccgacaa gtacgccacc
     4861 ttgaccgtgt acaacacggc cggcaccagg gttgaggaaa cctacagccc gacgcggccc
     4921 gtcgtgctga acaacggcgg cattgtcgtc gcccggaccc tcaccagcac cgtttacgac
     4981 gacgaagccg acaccagcct gacgccaggg cgccccagca gcggcgtccc cgacggcggc
     5041 taccaactcg ccgtcgagca gacgacggca gtcactgacc gaatcctgcc caaagcagac
     5101 ggcaacacct gggacaccaa caagacccgg taccgctacg accctgtcgt cgccgccgac
     5161 ccgtccggct ggctgttgaa ggtgccaacc aggaccctca cccaggacgg ctcgggatgg
     5221 gccaccaccc tgacccgcta cgacagcaac ggccggatcg tcgagacccg caccccgggc
     5281 ggcaccgcca tcaccaacgg gaccgcgaac gacacctact ccaccaagac catctactac
     5341 accgccgaca cctcagcgtc ggtcagcgcc tgccgatcca aacccgaatg ggacggcaat
     5401 gcctgcatcg tcaagactgg cggcgacccg tccaccggct accccatccc cgccaaaaca
     5461 accaccggct acagcatctt cggagccgtc actcgactcg aagagaccgg gtccacctgg
     5521 agccgtgcca ccatcaccgg attcgactac cagggccggg aaacctccgg cagcaccgcc
     5581 ctcaccgatc acaccacgat cagcggcacc accagctacc acgccaccac cggggcggtc
     5641 accgccacca cccgaggcgg catcaccgag aacttcactc acgacagctg ggggcgcgtc
     5701 ctcaccagca ccgacggcac cggcaacacg gccaccacca gctacgacac agccggacgc
     5761 accaagacct tcaacgacgg caaaggcagc tacacctaca gctacgacgg caccgacaac
     5821 ctcggacaag tcgaacatc
//
LOCUS       DAOSUG010000147         5820 bp    DNA     linear   ENV 10-JUN-2024
DEFINITION  MAG TPA_asm: Micropruina sp. isolate AMR_MDS_2195
            NODE_6334_length_5820_cov_3.675108, whole genome shotgun sequence.
ACCESSION   DAOSUG010000147 DAOSUG010000000
VERSION     DAOSUG010000147.1
DBLINK      BioProject: PRJNA925477
            BioSample: SAMN32864262
            Sequence Read Archive: SRR4236673
KEYWORDS    WGS; ENV; Metagenome Assembled Genome; MAG; Third Party Data; TPA;
            TPA:assembly.
SOURCE      Micropruina sp. (wastewater metagenome)
  ORGANISM  Micropruina sp.
            Bacteria; Bacillati; Actinomycetota; Actinomycetes;
            Propionibacteriales; Nocardioidaceae; Micropruina.
REFERENCE   1  (bases 1 to 5820)
  AUTHORS   Abdulkadir,N., Saraiva,J.P., Zhang,J., Stolte,S., Gillor,O.,
            Harms,H. and Rocha,U.
  TITLE     Genome-centric analyses of 165 metagenomes show that mobile genetic
            elements are crucial for the transmission of antimicrobial
            resistance genes to pathogens in activated sludge and wastewater
  JOURNAL   Microbiol Spectr 12 (3), e0291823 (2024)
   PUBMED   38289113
REFERENCE   2  (bases 1 to 5820)
  AUTHORS   Abdulkadir,N.
  TITLE     Direct Submission
  JOURNAL   Submitted (24-MAR-2023) Environmental Microbiology, Helmholtz
            Center for Environmental Research -UFZ, Permoserstr 15, Leipzig
            04318, Germany
COMMENT     The annotation was added by the NCBI Prokaryotic Genome Annotation
            Pipeline (PGAP). Information about PGAP can be found here:
            https://www.ncbi.nlm.nih.gov/genome/annotation_prok/
            
            ##Genome-Assembly-Data-START##
            Assembly Method        :: SPAdes v. 3.11.1
            Genome Representation  :: Full
            Expected Final Version :: Yes
            Genome Coverage        :: Not Applicable
            Sequencing Technology  :: Illumina MiSeq
            ##Genome-Assembly-Data-END##
            
            ##Genome-Annotation-Data-START##
            Annotation Provider               :: NCBI
            Annotation Date                   :: 05/04/2023 04:57:10
            Annotation Pipeline               :: NCBI Prokaryotic Genome
                                                 Annotation Pipeline (PGAP)
            Annotation Method                 :: Best-placed reference protein
                                                 set; GeneMarkS-2+
            Annotation Software revision      :: 6.5
            Features Annotated                :: Gene; CDS; rRNA; tRNA; ncRNA
            Genes (total)                     :: 2,776
            CDSs (total)                      :: 2,750
            Genes (coding)                    :: 2,714
            CDSs (with protein)               :: 2,714
            Genes (RNA)                       :: 26
            tRNAs                             :: 24
            ncRNAs                            :: 2
            Pseudo Genes (total)              :: 36
            CDSs (without protein)            :: 36
            Pseudo Genes (ambiguous residues) :: 0 of 36
            Pseudo Genes (frameshifted)       :: 2 of 36
            Pseudo Genes (incomplete)         :: 32 of 36
            Pseudo Genes (internal stop)      :: 2 of 36
            ##Genome-Annotation-Data-END##
FEATURES             Location/Qualifiers
     source          1..5820
                     /organism="Micropruina sp."
                     /mol_type="genomic DNA"
                     /submitter_seqid="NODE_6334_length_5820_cov_3.675108"
                     /isolate="AMR_MDS_2195"
                     /isolation_source="Activated sludge of municipal
                     wastewater treatment plants"
                     /db_xref="taxon:2737536"
                     /environmental_sample
                     /geo_loc_name="Canada"
                     /lat_lon="49.25 N 123.1 W"
                     /collection_date="2015-06-16"
                     /metagenome_source="wastewater metagenome"
                     /note="metagenomic"
     gene            complement(<1..354)
                     /locus_tag="PLF56_06165"
     CDS             complement(<1..354)
                     /locus_tag="PLF56_06165"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_013160575.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="hypothetical protein"
                     /protein_id="HOQ53184.1"
                     /translation="MTVRDLASSAWFRVNEDLQTALREDPAAHSKLMVALAYPGVHAV
                     WVHRLCHKLWKSSPALRPLARLLSQVSRTLTQVEIHPGAVIGRRFFIDHGNGVVIGET
                     AEIGDDVLMYHQVTLG"
     gene            complement(652..1581)
                     /gene="cysK"
                     /locus_tag="PLF56_06170"
     CDS             complement(652..1581)
                     /gene="cysK"
                     /locus_tag="PLF56_06170"
                     /EC_number="2.5.1.47"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_011757733.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_function: GO:0004124 - cysteine synthase activity
                     [Evidence IEA];
                     GO_process: GO:0006535 - cysteine biosynthetic process
                     from serine [Evidence IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="cysteine synthase A"
                     /protein_id="HOQ53185.1"
                     /translation="MQIFDDVTELVGRTPLVRLHRIFGDTQATVLAKLEFYNPANSVK
                     DRIGVAMIDAAEASGQLKPGGTIVEATSGNTGIALAMVGAARGYRVVLTMPETMSKER
                     RALLRAFGAELVLTPGPEGMRGAVARAEAIGAEEGAVLVRQFANPANVDIHRKTTAEE
                     IWNDTDGTVDIVVAGIGTGGTITGVGQVLKERKPEVTIVAVEPKESAILSGGQPGPHK
                     IQGIGANFVPEILDRDVIDEILPQNAETAVEFARLAAAQEGLLVGISSGAALASAGEL
                     AAREENAGKVIVVIIPSFGERYLSTILYADLLD"
     gene            1676..3229
                     /gene="guaA"
                     /locus_tag="PLF56_06175"
     CDS             1676..3229
                     /gene="guaA"
                     /locus_tag="PLF56_06175"
                     /EC_number="6.3.5.2"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_002519617.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_function: GO:0003922 - GMP synthase
                     (glutamine-hydrolyzing) activity [Evidence IEA];
                     GO_function: GO:0005524 - ATP binding [Evidence IEA];
                     GO_function: GO:0016462 - pyrophosphatase activity
                     [Evidence IEA];
                     GO_process: GO:0006177 - GMP biosynthetic process
                     [Evidence IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="glutamine-hydrolyzing GMP synthase"
                     /protein_id="HOQ53186.1"
                     /translation="MTHEHELVLVVDFGAQYAQLIARRVREARIYSEIVPHTASVAEI
                     MARKPKAIVLSGGPQSVYAPGAPQVDPALFTTGTPTFGMCYGFQAMASALGGEVRRTG
                     LSEFGRTSVHVGEGGALLDALPPNFRAWMSHGDAVYAPPPGFTCLATSDGSPVAAFED
                     LDRGLAGVQWHPEVGHSEHGQAVLERFLVQIAGCSQDWTPASIVDDSVAAIRAEVGDA
                     QVLCALSGGVDSAVAAALVQKAIGDQLTCVFVDHGLLRKGEAERVERDFVAVTGVDLV
                     VRHETDRFLGALAGVSDPETKRKIIGREFIRTFEDAARALNASKDIEYLVQGTLYPDV
                     VESGGGEGAANIKSHHNVGGLPDDLQFKLIEPLRTLFKDEVRAVGTELGLPDDMVWRH
                     PFPGPGLAIRIIGAVTAERLDLLREVDAIARSELTAAGLDREIWQFPVVLLAEVRSVG
                     VQGDGRTYGHPVVLRPVTSEDAMTADWGRLPYEVLEKISTRITNEVREVNRVVLDITS
                     KPPGTIEWE"
     gene            complement(3755..4600)
                     /locus_tag="PLF56_06180"
                     /pseudo
     CDS             complement(3755..4600)
                     /locus_tag="PLF56_06180"
                     /inference="COORDINATES: protein motif:HMM:NF012546.2"
                     /note="internal stop; Derived by automated computational
                     analysis using gene prediction method: Protein Homology.
                     GO_function: GO:0008236 - serine-type peptidase activity
                     [Evidence IEA];
                     GO_process: GO:0006508 - proteolysis [Evidence IEA]"
                     /pseudo
                     /codon_start=1
                     /transl_table=11
                     /product="prolyl oligopeptidase family serine peptidase"
BASE COUNT          868 a         2031 c         2039 g          882 t
ORIGIN      
        1 acccagggtc acctggtgat acatcaacac gtcgtcgccg atctcggcag tctcgccgat
       61 caccactccg ttgccgtgat cgatgaagaa gcggcgcccg atcaccgctc cggggtggat
      121 ctcgacctgg gtcagcgtgc gcgacacctg tgagagcagc cgggccagcg ggcgcagcgc
      181 cgggctgctt ttccacagtt tgtggcacag ccggtgcacc cacaccgcgt gtacgcccgg
      241 gtacgccagc gccaccatga gtttgctgtg cgcggccggg tcttcgcgca gcgccgtctg
      301 caggtcttcg ttcacccgaa accaggcgga cgaagccagg tctcgcaccg tcatcggcac
      361 cgtccaggca aggtcatcgc accatccaag caggtgccgg gccagccgtc gcacccgtcc
      421 accacgcgcg acggccccgt cgcctggacg gggccgcctc gaagcctgga tccaccgatg
      481 ggcgccgtcg cgagcggcac cagacgggtg gcctggcaag ccggtccgcg cgaaggcata
      541 ccgggcgtct ggtgagtgtg gccgacgccg ccagggcgcc cggacggggc cgcgcagtag
      601 gcgcagatcg gtggatccag gctaagcggt ggccaggcca ccgcgccacg atcagtcgag
      661 caggtcggcg tacaggatcg tcgacaggta gcgttcgccg aacgacggaa tgatgaccac
      721 gatcaccttg ccggcgttct cttcgcgggc cgccaactca ccggcggacg ccagcgcggc
      781 ccccgagctg atgccgacca gcaggccctc ctgggcagcg gccagccggg cgaactcgac
      841 ggccgtctcg gcgttctgcg gcaggatctc gtcgatcacg tcgcggtcga ggatctcggg
      901 cacgaagttc gcgccgatgc cctgaatctt gtgcggaccg ggctggccgc cggacaggat
      961 cgccgactcc ttgggctcga cggccacgat cgtgacctcg ggcttgcgtt ccttgagcac
     1021 ctggccgaca ccggtgatgg tgccaccggt gccgatgccg gccaccacga tgtcgacggt
     1081 gccgtcggtg tcgttccaga tctcttcggc ggtcgtcttg cggtggatgt cgacgttggc
     1141 cgggttggcg aactggcgca ccagcacggc gccctcttcg gcgccgatcg cctcggcccg
     1201 cgccaccgcg ccccgcatgc cctcagggcc gggggtgagc acgagttcgg caccgaacgc
     1261 ccgcagcagc gcccggcgct ccttgctcat cgtctcgggc atggtcagca ccacccggta
     1321 gccgcgcgcc gcgcccacca tggccagcgc gatgccggtg ttgcccgagg tcgcctcgac
     1381 gatcgttccg cccggcttca gttggccgga cgcctcggcg gcgtcgatca tcgcaacgcc
     1441 gatgcggtcc ttgaccgagt tggccgggtt gtagaactcg agcttggcca gcacggtcgc
     1501 ctgggtgtcg ccgaagatgc ggtggaggcg caccagcggg gtgcgtccga ccagctcggt
     1561 gacgtcgtcg aagatctgca tgggttgtcc taaccggtgt tgggcgcggc tgtgcccgcg
     1621 ttcatgaatg actgttcaaa catagtacct attgctttta cctacactgc gtcccatgac
     1681 ccacgagcac gagttggtac tggtcgtcga cttcggtgcc cagtacgcgc agctgatcgc
     1741 gcggcgcgtc cgagaggccc ggatctattc cgagatagtg ccgcacacgg cgtccgttgc
     1801 cgagatcatg gcgcgcaaac ccaaggcgat cgtgctctcg ggcgggccgc agtcggtgta
     1861 cgcgcccggc gctccgcagg tcgacccggc gctgttcacc accggcaccc cgaccttcgg
     1921 catgtgctac gggttccagg cgatggcgtc cgccctgggc ggcgaggtgc gccgcaccgg
     1981 gctgtcggag ttcggccgca cgtccgtcca cgtgggcgag ggtggtgcgc tgctggacgc
     2041 gctgccgccg aacttccggg cctggatgag ccatggcgat gcggtgtacg cgccgccgcc
     2101 ggggttcacc tgcctcgcca cctcggacgg gtcgccggtc gccgcgttcg aggatctcga
     2161 ccgcggcttg gcaggagtgc agtggcaccc cgaggtgggg cactccgaac acggccaggc
     2221 ggtgctggaa aggttcctgg tgcagatcgc cggttgcagc caggactgga ccccggccag
     2281 catcgtcgac gactcggtgg cagcgatccg ggccgaggtg ggcgatgcgc aggtgctgtg
     2341 cgcgctgtcc gggggcgtcg actcggcggt cgccgcggcc ctggtgcaga aggcgatcgg
     2401 cgatcagctc acctgtgtgt tcgtcgatca cgggctgctg cgcaagggcg aggccgagcg
     2461 ggtcgagcgc gacttcgtgg ccgtcaccgg cgtcgacctg gtggtgcgcc acgaaaccga
     2521 ccggttcctg ggcgcgctgg ccggggtgag cgaccccgag accaagcgca agatcatcgg
     2581 acgcgagttc atccgtacct tcgaggacgc cgcccgggcg ctgaatgcgt ccaaggacat
     2641 cgagtacctg gtgcagggca cgctctaccc cgacgtggtc gaatccggcg ggggtgaggg
     2701 cgcggccaac atcaagagcc accacaacgt cggcggcctg cccgacgacc tgcagttcaa
     2761 gctgatcgag ccgctgcgca ccctgttcaa ggacgaggtg cgcgccgtcg gcaccgaact
     2821 gggcttgccg gacgacatgg tgtggcggca cccgttccca ggccccggcc tggcgatccg
     2881 gatcatcggc gcggtcacgg ccgagcggct ggacctgctg cgcgaagtgg acgcgatcgc
     2941 ccggtccgaa ctgaccgctg ccgggctgga ccgcgagatc tggcagtttc cggtggtgct
     3001 gctggctgag gtccgttcgg tcggcgtgca gggcgacggc cgcacgtacg ggcacccggt
     3061 ggtgctgcgt ccggtcacct ccgaggacgc gatgaccgcc gactggggcc ggctgcccta
     3121 cgaggtgctc gagaagatca gtacccggat caccaacgag gtgcgcgagg tcaaccgggt
     3181 ggtgctcgac atcacgtcca agccgccggg gacgatcgag tgggagtgag gcggtaggtc
     3241 gcgccggctg cgcgttttct gcacctgggt atctggctga aagtctgctg ccgcgcggcc
     3301 gggacggtgc ccgagccgcc cacctccgga tcgccctcgc aagctcggcc gatcctccga
     3361 cgcccaccca cctgcctcgg gcaccgtccc ggccgcgctt tcgctcttct gtctgcgtcg
     3421 tctgcattgc ccgtcggttg ttagcctgca cctcaatggc cggcgcgatg cacctggttc
     3481 cggacttggg gtggttcgcg aggcgcgcgg tcgcgggcga tgtcgccggt cgagcgcacc
     3541 gacggtcgag ccctacggcc ggtcgggccg gtcgaacact cggccggtcc agccctacgg
     3601 ccggtccagc cctacggccg tccagcccta cggccggtcg ggccggtcga gccctcccgt
     3661 ccgtcgagcc gaccgccaag gccattgcgc ggggcgtgat ctcgacaggc tcgatctcga
     3721 caggctcgac cgatgacagg ctcgatccac cggctcacac cggcaggtac cgcgcccacc
     3781 aggtcaggat cgcctcgaac cgttgccgcc gatgccaggg ggtgcccgag cgtgacaact
     3841 cgtgggtttc gccggggaac accagcagct cggcggccac accgtcctgt ttcagctgcg
     3901 tatagtaccg cagggcctgt cacaaggggc aacgcaggtc gagctcggaa tggatcacca
     3961 gcgtgggcgt ggtcacccgg tcggtgagca gcatcggcga ctgtgcgtcc atctgctctt
     4021 tcgagccgtt gtactgcgcc ggaaagaacc acccgatgtc ggacgcaccc acgaacgacg
     4081 ccgggtcgag gtagccgcgc tcgacgatcg cccccgcgaa acggtgctcg tgggcgatga
     4141 tccacgcggt cagataaccg ccgtaggagc cccccatgat gccgacccgg gcgccgtcca
     4201 gccccggcac ggtcgcgatc gcgtgatcga gaaacgccag cacatcggcc gaatcgaggt
     4261 tgccgaaatt ggtgcggatc gctcggccgt ggtcgcgtcc gtaccccgcg gaaccgcgtg
     4321 ggttgcacag caccaccgca tacccggcct cggcgtacac ctgcgcctcg tcgaagaatg
     4381 ccgacgtgta ctgcgcgaac ggcccgccgt ggatcagcag cagcgccggg tgcggaccct
     4441 cgccgccggg gctcaccacc cagccgtgca ccgggtggcc gtcgggtgcg gcggcggtga
     4501 gctcctgcgg ctcgcgcacc ctcgccgccg cccgcaacgc ggccgagaag tcggtcagcg
     4561 gacgcaccgt gcccgcctcg agcagcccca cctcgtccat gcccacgctg gtgcggtagg
     4621 tgaccatgat cggtgaatca gcggccggac gggcgcccac cgtgccgcag ccgaaaatca
     4681 gcacgcccgc gtcgctgtcg agcagcaact cggcgcctgc tccggtcacc cggaacacct
     4741 gactggcgcc ccggctctcg gcgaccgcga gcacaccgtc gacggtgggg gtgagcgggc
     4801 cctcgatgag cgactcggca tcggtgagca gcgccggcga cccgtcgtgc agccggtaga
     4861 cgcccgagtg tgccgccacg aagtccatgc ccgactcacc gaggtcggtg ccgacgaacc
     4921 agatgtcgtc accgctgacc agggggtggc tgacgtcgag gttcgcctcg gtcagccgga
     4981 cggtctcgga cccgtccacc gccacccgca ccaggtcgcc gaccagggtt tcgggcccct
     5041 cggtgcgggc cgcggtgacc agcacggcgg tgccgtccgc ggtgaaacag ggctgcgtga
     5101 agtcggtgtc gcccctggtc aaccggtggg ccggcggcac gccagggttc ttcagcgact
     5161 cggggtcgag tcccttcgct gcccgaccct tcggcgtgac ggtcggctcg ccgaacacgt
     5221 ccggaacgtt caccacgaac aggtggctgc gccggtcgcg tacccagccc agcccgttgg
     5281 actggaactg caggctggtg atgtgccgcg ggtcttcgtg accggcatcg acgtcgtcga
     5341 tgctgccgta gcggcccttg tcgggcaccc gggcgatgaa ggccagcgtg cgaccatcgg
     5401 gtgaaaaggc gaagtccgac acgccgaggg gctggtgggt cacgcagagt gcttcgccgc
     5461 cgccggaagc aaccaggtgg acctgcgccc gatcattggc accggcccgc agaaaggcga
     5521 tcagcgagcc gtccggcgaa aagagggggt gcgtgtcgtg ctggccgcgg gtcagccgac
     5581 gggcgcccga accgtccagc ggcacgcgga acaactggcc gacgtagtcg tcggcgtcga
     5641 agtcggcgcg gctggcggcc accacggccc aactgccgtc ggggtgcacc gccggggccg
     5701 aaagggtggt cagcagaggc agttggtccg gacgcatggc cccgacgcta ctacccgcca
     5761 ccgaccaact ccggcgctcg accgctgcgc gaccggtggg ctaggttcgt gcacagccca
//
LOCUS       DAOSUG010000148         5819 bp    DNA     linear   ENV 10-JUN-2024
DEFINITION  MAG TPA_asm: Micropruina sp. isolate AMR_MDS_2195
            NODE_6336_length_5819_cov_4.254164, whole genome shotgun sequence.
ACCESSION   DAOSUG010000148 DAOSUG010000000
VERSION     DAOSUG010000148.1
DBLINK      BioProject: PRJNA925477
            BioSample: SAMN32864262
            Sequence Read Archive: SRR4236673
KEYWORDS    WGS; ENV; Metagenome Assembled Genome; MAG; Third Party Data; TPA;
            TPA:assembly.
SOURCE      Micropruina sp. (wastewater metagenome)
  ORGANISM  Micropruina sp.
            Bacteria; Bacillati; Actinomycetota; Actinomycetes;
            Propionibacteriales; Nocardioidaceae; Micropruina.
REFERENCE   1  (bases 1 to 5819)
  AUTHORS   Abdulkadir,N., Saraiva,J.P., Zhang,J., Stolte,S., Gillor,O.,
            Harms,H. and Rocha,U.
  TITLE     Genome-centric analyses of 165 metagenomes show that mobile genetic
            elements are crucial for the transmission of antimicrobial
            resistance genes to pathogens in activated sludge and wastewater
  JOURNAL   Microbiol Spectr 12 (3), e0291823 (2024)
   PUBMED   38289113
REFERENCE   2  (bases 1 to 5819)
  AUTHORS   Abdulkadir,N.
  TITLE     Direct Submission
  JOURNAL   Submitted (24-MAR-2023) Environmental Microbiology, Helmholtz
            Center for Environmental Research -UFZ, Permoserstr 15, Leipzig
            04318, Germany
COMMENT     The annotation was added by the NCBI Prokaryotic Genome Annotation
            Pipeline (PGAP). Information about PGAP can be found here:
            https://www.ncbi.nlm.nih.gov/genome/annotation_prok/
            
            ##Genome-Assembly-Data-START##
            Assembly Method        :: SPAdes v. 3.11.1
            Genome Representation  :: Full
            Expected Final Version :: Yes
            Genome Coverage        :: Not Applicable
            Sequencing Technology  :: Illumina MiSeq
            ##Genome-Assembly-Data-END##
            
            ##Genome-Annotation-Data-START##
            Annotation Provider               :: NCBI
            Annotation Date                   :: 05/04/2023 04:57:10
            Annotation Pipeline               :: NCBI Prokaryotic Genome
                                                 Annotation Pipeline (PGAP)
            Annotation Method                 :: Best-placed reference protein
                                                 set; GeneMarkS-2+
            Annotation Software revision      :: 6.5
            Features Annotated                :: Gene; CDS; rRNA; tRNA; ncRNA
            Genes (total)                     :: 2,776
            CDSs (total)                      :: 2,750
            Genes (coding)                    :: 2,714
            CDSs (with protein)               :: 2,714
            Genes (RNA)                       :: 26
            tRNAs                             :: 24
            ncRNAs                            :: 2
            Pseudo Genes (total)              :: 36
            CDSs (without protein)            :: 36
            Pseudo Genes (ambiguous residues) :: 0 of 36
            Pseudo Genes (frameshifted)       :: 2 of 36
            Pseudo Genes (incomplete)         :: 32 of 36
            Pseudo Genes (internal stop)      :: 2 of 36
            ##Genome-Annotation-Data-END##
FEATURES             Location/Qualifiers
     source          1..5819
                     /organism="Micropruina sp."
                     /mol_type="genomic DNA"
                     /submitter_seqid="NODE_6336_length_5819_cov_4.254164"
                     /isolate="AMR_MDS_2195"
                     /isolation_source="Activated sludge of municipal
                     wastewater treatment plants"
                     /db_xref="taxon:2737536"
                     /environmental_sample
                     /geo_loc_name="Canada"
                     /lat_lon="49.25 N 123.1 W"
                     /collection_date="2015-06-16"
                     /metagenome_source="wastewater metagenome"
                     /note="metagenomic"
     gene            complement(<1..1238)
                     /locus_tag="PLF56_06185"
     CDS             complement(<1..1238)
                     /locus_tag="PLF56_06185"
                     /inference="COORDINATES: protein motif:HMM:NF013584.2"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_function: GO:0016810 - hydrolase activity, acting on
                     carbon-nitrogen (but not peptide) bonds [Evidence IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="amidase"
                     /protein_id="HOQ53187.1"
                     /translation="MTVVRVEEYCELTAIQIATAVNGRQLSAVEVAEAALAVAADRGP
                     RFGAFTCLAPERALSDARRTDARIASGERPLLAGVPCPIKDLNAVAGLPWEAGSAAMR
                     GNLAEADDPIVGWLGDAGTSMIGKTATPEFGLPCYTEPNGRPPTVTPWDPTRSAGGSS
                     GGASAAVAAGIAPIAHASDGGGSIRIPASACGLVGLKASRGLISAGARPPGPGLGTDG
                     VVSRTVRDTALALDVLAGPRAGDTYWAPVDASGYLAACGRPVERLRVGLLTTPVIAAD
                     AEVHPACLDAAARAALLLVGLGHDVSEAPVAFAAERWNAFRSVWAVGALGIPLPPEAE
                     PALTPLTRWLRAKGRTVSGLEYAGAMAAIQLLTSEVARTWADFDVILSPTLAQPPALV
                     GSLRDDPDPAGDFDAQTRFTP"
     gene            1322..2938
                     /locus_tag="PLF56_06190"
     CDS             1322..2938
                     /locus_tag="PLF56_06190"
                     /inference="COORDINATES: ab initio
                     prediction:GeneMarkS-2+"
                     /note="Derived by automated computational analysis using
                     gene prediction method: GeneMarkS-2+."
                     /codon_start=1
                     /transl_table=11
                     /product="hypothetical protein"
                     /protein_id="HOQ53188.1"
                     /translation="MLAGIRDETPHRTDPLVAPAAAVAPTTGRRRAVTARLSALLLFV
                     GAVAWFGWGVGTPEAWSDEAVTVLTIERPWSAVWSLWNSGDATLTPYYLALKAWSEAA
                     HWLGFAPGVTVARSFSVAAAAAATACLYLLVHRSAGELTALLAGVVFMALPGVSRYAQ
                     EARPYALLMAAAAFSWLLADSLWRAADAAPGQVRPRTRLTYTLSLVVLALTNLFGLLQ
                     WVAQACAALIGRPRWPRLRVPLPQAIAATGLVAVPVAIMATRGSGARVPSEVNAFTVT
                     DMLQVVALHVGAPPLLGLFLLLLTCCGLLGLRTQRLRGFVVRVLIWLVVPALLATVFA
                     LVRSQLFRVRYWMPAALPIAVLAAIGIAEVAGAVAGVWQRQADRSWAARATRVTVAAA
                     LLVALWVAMLPLNQRFRAPDGHDATLGPVIARILELRAEQPGMAVLVASPGQSVVFAT
                     SPAPGVYRQNVYLNTELRPESVYPAVESFARLSERLTDRDRVLVIRMVPTAASATSDE
                     TAAQRLAKLGFRATSDWTELSGWQLREYRR"
     gene            complement(3204..4562)
                     /locus_tag="PLF56_06195"
     CDS             complement(3204..4562)
                     /locus_tag="PLF56_06195"
                     /EC_number="1.6.5.-"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_014847727.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="NAD(P)/FAD-dependent oxidoreductase"
                     /protein_id="HOQ53189.1"
                     /translation="MPRPHVVIIGSGFGGLFSARALAKADVDITLLSKTTAHLFQPLL
                     YQVATGILSSGEIAPATRDILARQKNAKVLLSEVSKINLDSRIVIGEKLSKQTILRYD
                     YLIVAAGAGQSYFGNDQFATFAPGMKSIDDALELRARIFGALEMAELSQDAAERRSLM
                     TFVVVGAGPTGVELAGQIVELCHKTLVDTFRNIDTRQCRVLLLDAAPVVLPPFGEKLS
                     AAAHKELERIGVEVKLNAMVTNVDATGLDLKLKDGSTERVEAICKVWAAGVQASPLGK
                     TLAEQSGIEVDRAGRVPVQPDLTLPGHPAVFVIGDMMALDRLPGVAQVAIQGARYAAK
                     KIVGDLNGTPVTEKFSYKDKGSMAVISRFGAVAKINDKLQFSGFLAWLSWLALHLIYV
                     VGFKNRITTLLHWTITFLGSGRSERVTTEQQLVGRLAVKQLGEAFEPTISGILDPNKI
                     EH"
     gene            complement(4654..5580)
                     /locus_tag="PLF56_06200"
     CDS             complement(4654..5580)
                     /locus_tag="PLF56_06200"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_016456799.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_process: GO:0008299 - isoprenoid biosynthetic process
                     [Evidence IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="polyprenyl synthetase family protein"
                     /protein_id="HOQ53190.1"
                     /translation="MRIIEHRLSDSAFGQTPFVTEAASHIINAGGKRLRPMLVVVGAG
                     LHPGASADEIERAAVVVELTHVASLYHDDVMDEADLRRGAESANQRYDNSIAIMIGDF
                     LFARASSIVATLGVDYVALQADTFARLVQGQIAETRGPQAGDDPVAHYLQVVADKTGS
                     LIATSAVFGGMVCGATASELHALAAFGEELGVVFQLSDDIIDIASDETGKTPGTDLRE
                     GVPTLPTLLVKAGADPSDARLRQLLQGEITDPDDLAEALRLMRTHGALSEARAEVDRR
                     AQLAKSHLDALPSGNARDALIELCDQIVSRTT"
BASE COUNT          823 a         1994 c         2072 g          930 t
ORIGIN      
        1 ggcgtgaacc gggtctgcgc atcgaagtcg ccggccggat cggggtcgtc gcgcaggctg
       61 ccgaccaagg caggtggctg cgccagggtc gggctcagga tcacatcgaa atcggcccag
      121 gttcgagcaa cctcgctggt cagcagctga atagccgcca tggcgcccgc gtactccagg
      181 ccgctgaccg tgcggccctt cgcccgcagc caccgggtca gcggggtcag cgccggctcg
      241 gcctcgggcg gcagcggaat accgagggcg cccacggccc acaccgaccg gaacgcgttc
      301 cagcgctcgg cggcgaaggc gaccggcgcc tcgctcacgt cgtggcccag acctacgagc
      361 agcagggcgg cgcgcgcggc agcgtccagg caggcggggt gcacctcggc gtcggcggcg
      421 atcaccggcg tggtcagcag accgacgcgc agtcgctcca ccggacgacc gcaggccgcc
      481 agatagccgc tcgcatccac gggcgcccag tacgtgtcgc cggcgcgggg gccggccagc
      541 acgtcgaggg cgagcgcggt gtcgcggacc gtccggctca ccacaccgtc ggtgcccagc
      601 cccggcccgg gcggacgggc ccccgccgag atcagtcccc ggctagcctt caggcccacc
      661 aggccgcagg cggatgcggg aatgcggatc gagccgccgc cgtcagaggc gtgggcgatc
      721 ggcgcgatgc ccgccgcgac ggctgcggac gctcccccgc tggagccacc ggcggagcga
      781 gtcgggtccc agggtgtgac ggtcggcgga cgtccgttgg gctcggtgta gcaaggcagg
      841 ccaaactcgg gcgtcgcggt cttgccgatc atggacgtgc ccgcgtcgcc gagccagccg
      901 acgatcgggt cgtcggcctc ggcgaggttg ccacgcatgg cggcgctgcc ggcctcccag
      961 ggcagcccgg ccaccgcgtt cagatccttg atcgggcacg ggaccccagc gagcagcggc
     1021 cgctcgccgg aagcgatgcg tgcgtccgtg cgccgggcgt ccgacagtgc ccgctcgggc
     1081 gccagacacg tgaaggcacc gaagcgcgga ccacggtctg cggccaccgc cagcgccgcc
     1141 tcggcgacct cgacggccga caactgccgc ccgttcaccg ccgtggcgat ctggatcgcc
     1201 gtcagctcgc aatactcctc cacacgcaca accgtcatcc tggcacgccg atcccgagcg
     1261 cgtgccgcag tgccgtcgga cgtggcgcga gtgcgtccgg actcagacgt agactcaacg
     1321 ggtgctcgct ggtattcgcg acgagacccc ccaccgcacc gatccgctgg ttgcgccggc
     1381 cgcggccgtg gcgccgacca ccggccgccg cagagccgtg accgcacgcc tatcggcgct
     1441 gctgctgttc gtcggcgccg ttgcgtggtt cgggtggggc gtgggcaccc ccgaggcctg
     1501 gagcgacgag gccgtgaccg tgctcaccat cgaacgcccg tggtcggcgg tgtggagcct
     1561 gtggaacagc ggggacgcca cgctgacgcc gtactacctc gccctgaagg cctggagcga
     1621 ggcggcccac tggctcggat tcgcaccggg ggtgaccgtg gctcgcagct tctcggtggc
     1681 cgccgccgcg gctgccacgg cgtgcctgta cctgctggtg caccgcagcg cgggcgaact
     1741 caccgcactg ctggccggtg tggtgttcat ggcactgccg ggagtgtcga gatatgccca
     1801 ggaggcccgt ccctacgcgc tgttgatggc ggccgccgcg ttttcgtggt tgctggccga
     1861 ttcgctctgg cgggcggcgg acgcggcccc cggacaggtg cgcccccgga ccaggctcac
     1921 ctacacgctg agcctggtgg tgcttgcgct gaccaacctg ttcggtctgc tgcagtgggt
     1981 ggcccaagcc tgcgccgcgc tgatcggacg gccccgctgg ccgcggctgc gcgtgccgct
     2041 gccgcaggcg atcgccgcaa ccgggctggt cgctgtgccg gtcgcgatca tggcgacccg
     2101 cgggtccggg gcgcgcgttc cgtccgaggt gaacgcgttc accgtgaccg acatgctgca
     2161 ggtggtggcg ctgcacgtcg gcgccccacc cttgctcggg ctctttctgc tgctgctgac
     2221 ctgctgcggg ctgctcggcc tgcggacgca acggctgcgc ggcttcgtgg tgcgggtgct
     2281 gatctggctc gtggttccgg ccctgttggc cacggtgttc gcgctggttc gaagccagct
     2341 gttccgggtg cggtactgga tgccggccgc cctgccgatc gccgttctcg cggccatcgg
     2401 catcgccgaa gtggccggtg cagtggcggg ggtgtggcag cgtcaggccg accgttcgtg
     2461 ggcggcgcgg gcgacccgcg tcaccgtggc cgccgccctg ctggtcgcgc tgtgggtggc
     2521 catgttgccg ctgaaccagc ggttccgggc accggacggg cacgatgcca cgctcgggcc
     2581 cgtcatcgcg cgcattctcg agctacgcgc cgagcaaccc ggcatggcgg tgctcgtcgc
     2641 ctcccccggt cagtcggtcg tcttcgccac ctcgcctgct cccggggtgt atcggcagaa
     2701 cgtgtacctg aatacggagc tgaggcctga gtcggtgtac cccgccgtcg aatcgttcgc
     2761 gcggctgagc gagcgactca ccgacaggga tcgggtgctg gtgattcgca tggtgcccac
     2821 ggccgcctcg gccacctccg acgagacggc tgcgcaacga ctggcgaaac tcgggttccg
     2881 tgcgacctcc gactggacgg agctgtcggg ctggcaactg cgcgagtatc gtcgctgatc
     2941 ccttgaacgg ctggactccc cttcagagac cgggcaggtt gctaggcggg ccgctacaca
     3001 ctcgatcagc ggaccgatct caacacactc gatcaacgca gccgcgcctg gacgtcggga
     3061 tctcgacaca ctggacgccc ggcacctgga tctcgacaac ctcgatcagc gaacctgggc
     3121 ttcgctcgtc ggaacccggc gctcgccacc ccggtcgccg gcgccccggg tgtggccggg
     3181 gcgtcccgga cgcgccgtca gcctcagtgc tcgatcttgt tcgggtcgag aatgccgctg
     3241 atcgtcggtt cgaaggcctc gcccagctgc ttgacggcca ggcgtccgac cagttgctgc
     3301 tcggtggtga cgcgctcgga gcgtccgctg ccgaggaagg tgatggtcca gtgcagcagg
     3361 gtggtgatgc ggttcttgaa gcccaccacg tagatcaggt gcagcgcgag ccacgacagc
     3421 caggcgagga agccgctgaa ctgcagcttg tcgttgatct tcgcgacggc gccgaagcgg
     3481 ctgatcaccg ccatggagcc cttgtccttg tagctgaact tctcggtcac cggcgtgccg
     3541 ttgaggtcgc cgacgatctt ctttgccgca tatcgggcgc cctgaatggc cacctgcgcc
     3601 acgccgggca gccggtcgag cgccatcatg tcgccgatca cgaacaccgc ggggtggccg
     3661 ggcagggtca ggtcgggctg gacgggcacg cggccggccc ggtcgacctc gatgcctgat
     3721 tgctcggcga gagtcttgcc caacggactg gcctgcacac ctgccgccca gaccttgcag
     3781 atcgcctcga cgcgttcggt ggagccgtct ttgagcttca ggtcgagccc ggtggcgtcc
     3841 acattggtca ccatcgcgtt cagcttgacc tcgacaccga tgcgctccaa ctctttgtgc
     3901 gcagcggcgg acagtttctc gccgaacggc ggcagcacca ccggtgccgc gtcgagcagc
     3961 agcacccggc actgccgggt gtcgatgttg cggaacgtgt caacgagcgt cttgtggcac
     4021 agttcgacga tctgaccggc cagctcgaca ccggtggggc cggcgccgac gaccacgaac
     4081 gtcatcagcg agcgacgttc ggcggcgtcc tggctgagct cggccatttc gagcgcaccg
     4141 aagatgcggg cgcgcaactc gagcgcgtcg tcgatgctct tcatgcccgg ggcgaaggtg
     4201 gcgaactggt cgttgccgaa gtacgactgg ccggcgccgg cggccacgat caggtagtcg
     4261 tagcgcagaa tggtctgctt gctgagcttt tcgccgatca cgatgcgcga gtcgaggttg
     4321 atcttgctga cctcgctgag cagcaccttg gcgttcttct gccgggccag aatgtcgcgg
     4381 gtggccggtg cgatctcacc gctggacaga atgcccgtgg ccacctggta cagcagcggt
     4441 tgaaacaggt gagcggtggt cttgctgagc agtgtgatgt cgacgtcggc cttggccagc
     4501 gcacgcgccg agaacagacc gccgaagccg gagccgatga tgacgacgtg aggacggggc
     4561 atgactactc caggggttgt gctgctcggt cggtcgggtt gagtctgccg tccagtgcga
     4621 gggctggcaa gcgtgcaacg ttgcgctgga cgatcaggtc gtgcgtgaga ctatctggtc
     4681 gcacagctcg atcagggcat cgcgggcgtt gccggacggc agcgcgtcca ggtggctctt
     4741 ggccagttgt gcccgccgat cgacttcggc gcgggcctcg ctcagtgcac catgggtgcg
     4801 catcagccgc agcgcctcgg ccaggtcgtc cgggtcggtg atttcaccct ggagcagttg
     4861 tcgcagccgc gcatcggacg ggtcggcgcc cgccttgacc agcagtgtcg gcagcgtggg
     4921 gacgccctcg cgcaggtcgg tgccgggggt cttgccggtc tcatcgctgg cgatgtcgat
     4981 gatgtcgtcg ctgagttgga agaccacgcc gagctcttcg ccgaacgcgg ccagcgcgtg
     5041 cagttcggac gcggtggcgc cgcacaccat gccgccgaac accgcggacg tcgcgatcag
     5101 cgagccggtc ttgtcggcca ccacctgcag gtagtgcgcg actggatcgt cgccggcctg
     5161 cgggccacgc gtctcggcga tctggccctg gacgaggcgg gcgaacgtgt cggcctgcag
     5221 cgcgacgtag tcgacgccga gggtggccac gatcgaggag gcccgggcga acagaaagtc
     5281 gccgatcatg atcgcgatcg agttgtcgta gcgctggttg gcactctcgg cgcctcgtcg
     5341 cagatcggcc tcgtccatca cgtcgtcgtg gtaaaggctg gccacgtggg tcagctcgac
     5401 caccaccgcg gcacgctcga tctcgtcggc cgaggcaccc ggatgcaggc ccgcacccac
     5461 caccacgagc atcggacgca gccgcttgcc gccggcgttg atgatgtgcg aggccgcctc
     5521 ggtgacgaac ggggtctggc cgaacgccga gtcgctgagc cgatgctcga tgatcctcag
     5581 gctgtcagcg acgctctgtg cgaacgtctc gtcgacaccg atctcgaggg tcacacttct
     5641 cctagctcag ggcacccgat gtgggaccga cagaaagcga acgtcaccca gcttagcctg
     5701 gatccaccga tgggcgccgt cgcgagcggc accagacggg tggcctggca agccggtccg
     5761 cgcgaaggca taccgggcgt ctggtgagcg tggccgacgc agccagggtg cccggatgg
//
LOCUS       DAOSUG010000149         5808 bp    DNA     linear   ENV 10-JUN-2024
DEFINITION  MAG TPA_asm: Micropruina sp. isolate AMR_MDS_2195
            NODE_6345_length_5808_cov_4.765514, whole genome shotgun sequence.
ACCESSION   DAOSUG010000149 DAOSUG010000000
VERSION     DAOSUG010000149.1
DBLINK      BioProject: PRJNA925477
            BioSample: SAMN32864262
            Sequence Read Archive: SRR4236673
KEYWORDS    WGS; ENV; Metagenome Assembled Genome; MAG; Third Party Data; TPA;
            TPA:assembly.
SOURCE      Micropruina sp. (wastewater metagenome)
  ORGANISM  Micropruina sp.
            Bacteria; Bacillati; Actinomycetota; Actinomycetes;
            Propionibacteriales; Nocardioidaceae; Micropruina.
REFERENCE   1  (bases 1 to 5808)
  AUTHORS   Abdulkadir,N., Saraiva,J.P., Zhang,J., Stolte,S., Gillor,O.,
            Harms,H. and Rocha,U.
  TITLE     Genome-centric analyses of 165 metagenomes show that mobile genetic
            elements are crucial for the transmission of antimicrobial
            resistance genes to pathogens in activated sludge and wastewater
  JOURNAL   Microbiol Spectr 12 (3), e0291823 (2024)
   PUBMED   38289113
REFERENCE   2  (bases 1 to 5808)
  AUTHORS   Abdulkadir,N.
  TITLE     Direct Submission
  JOURNAL   Submitted (24-MAR-2023) Environmental Microbiology, Helmholtz
            Center for Environmental Research -UFZ, Permoserstr 15, Leipzig
            04318, Germany
COMMENT     The annotation was added by the NCBI Prokaryotic Genome Annotation
            Pipeline (PGAP). Information about PGAP can be found here:
            https://www.ncbi.nlm.nih.gov/genome/annotation_prok/
            
            ##Genome-Assembly-Data-START##
            Assembly Method        :: SPAdes v. 3.11.1
            Genome Representation  :: Full
            Expected Final Version :: Yes
            Genome Coverage        :: Not Applicable
            Sequencing Technology  :: Illumina MiSeq
            ##Genome-Assembly-Data-END##
            
            ##Genome-Annotation-Data-START##
            Annotation Provider               :: NCBI
            Annotation Date                   :: 05/04/2023 04:57:10
            Annotation Pipeline               :: NCBI Prokaryotic Genome
                                                 Annotation Pipeline (PGAP)
            Annotation Method                 :: Best-placed reference protein
                                                 set; GeneMarkS-2+
            Annotation Software revision      :: 6.5
            Features Annotated                :: Gene; CDS; rRNA; tRNA; ncRNA
            Genes (total)                     :: 2,776
            CDSs (total)                      :: 2,750
            Genes (coding)                    :: 2,714
            CDSs (with protein)               :: 2,714
            Genes (RNA)                       :: 26
            tRNAs                             :: 24
            ncRNAs                            :: 2
            Pseudo Genes (total)              :: 36
            CDSs (without protein)            :: 36
            Pseudo Genes (ambiguous residues) :: 0 of 36
            Pseudo Genes (frameshifted)       :: 2 of 36
            Pseudo Genes (incomplete)         :: 32 of 36
            Pseudo Genes (internal stop)      :: 2 of 36
            ##Genome-Annotation-Data-END##
FEATURES             Location/Qualifiers
     source          1..5808
                     /organism="Micropruina sp."
                     /mol_type="genomic DNA"
                     /submitter_seqid="NODE_6345_length_5808_cov_4.765514"
                     /isolate="AMR_MDS_2195"
                     /isolation_source="Activated sludge of municipal
                     wastewater treatment plants"
                     /db_xref="taxon:2737536"
                     /environmental_sample
                     /geo_loc_name="Canada"
                     /lat_lon="49.25 N 123.1 W"
                     /collection_date="2015-06-16"
                     /metagenome_source="wastewater metagenome"
                     /note="metagenomic"
     gene            <1..373
                     /locus_tag="PLF56_06205"
     CDS             <1..373
                     /locus_tag="PLF56_06205"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_020578409.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_function: GO:0005524 - ATP binding [Evidence IEA]"
                     /codon_start=2
                     /transl_table=11
                     /product="ATP-binding cassette domain-containing protein"
                     /protein_id="HOQ53191.1"
                     /translation="VDGSIPAQLRLLLEVVARRLGESDAAVVRTLLAKFGLGPDHVGR
                     SCDSLSLGERTRAALAVLQGRAVNVLVLDEPTNHLDAAAIEQLQHALVAFEGTVLIVT
                     HDRALMDALAPVTIVPVNGRT"
     gene            complement(463..888)
                     /locus_tag="PLF56_06210"
     CDS             complement(463..888)
                     /locus_tag="PLF56_06210"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_002518125.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="organic hydroperoxide resistance protein"
                     /protein_id="HOQ53192.1"
                     /translation="MKILYTAVATATGEGRNGHVRSSDGILEADLATPTELGGTGGKT
                     NPEQLFAAGYSACFLNALKRMAGNAHVPITDAAVTAEVGMGRHPEDTGFVLAVTLVIE
                     VGGVDQQSAEQLAEQAHAVCPYSNATRGNIEVTLDVVTA"
     gene            1022..2488
                     /gene="lysS"
                     /locus_tag="PLF56_06215"
     CDS             1022..2488
                     /gene="lysS"
                     /locus_tag="PLF56_06215"
                     /EC_number="6.1.1.6"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_016668357.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_component: GO:0005737 - cytoplasm [Evidence IEA];
                     GO_function: GO:0004824 - lysine-tRNA ligase activity
                     [Evidence IEA];
                     GO_process: GO:0006430 - lysyl-tRNA aminoacylation
                     [Evidence IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="lysine--tRNA ligase"
                     /protein_id="HOQ53193.1"
                     /translation="MQVRRDKRARLLEAGTDPYPISVGRTHSAAQVHAQWGHLAAGEE
                     TQDVVGVSGRVVFIRNTGKLCFATLQDGFTREDSGTRLQVMLSLAEVGEQRLADWKAD
                     VDLGDFVFVQGRVISSKRGELSVLASQWRMASKALRPLPTLHKDLGEETRVRQRYADL
                     VARPEARDMVRTRATIVRSIRETLHSQCYLEVETPILQLVHGGAAARPFHTHLNAFDI
                     PMTLRIAIELYLKRAIVGGADRVYEMGRIFRNEGIDSSHSAEFTMLEAYQSWGDQFTI
                     AAMTRDLIVNAADALGVESVPSPLGEVHLTGDWQWLSVYPALSDALGEQITPDTPLQR
                     LREIADARSIEYDPAWIDQKLVMELFAEIVEPTLVQPTFVNGYPAIAQPLARNNPDDP
                     RLVEAWDLIIAGVERGTGFSELIDPVIQRDVLVAQSLKAAAGDPEAMQLDEDFLRALE
                     FGAPPMGGMGLGIDRTIMLFTGAGIRETILFPLLKPHG"
     gene            complement(2784..4172)
                     /locus_tag="PLF56_06220"
     CDS             complement(2784..4172)
                     /locus_tag="PLF56_06220"
                     /inference="COORDINATES: ab initio
                     prediction:GeneMarkS-2+"
                     /note="Derived by automated computational analysis using
                     gene prediction method: GeneMarkS-2+."
                     /codon_start=1
                     /transl_table=11
                     /product="hypothetical protein"
                     /protein_id="HOQ53194.1"
                     /translation="MAVVVACVLALGVTSTVLIWASRKGAVMSGFAVLVGMVAGTLAL
                     WALGGGTTLIMIAVPAALLLGLADAVLTARGVHWFWRLIVQGVVIFGVLVLEWRRGDL
                     FASGAVMATVTGVLMYNVINFAIGAGQRSGARRLPVGLWLLGAVYLVPIAVGLPNPGL
                     RTLVLVVIAGVLPLLIWPAPTGFLDRALGPVLAGLGCALAFYAWLGNASPAMVAAPLL
                     VVAVDVVYTLVARVVTAEGRAGLAGERTMASSAGTDGSLAADTSAASSGSGGSAAAFG
                     SGGSAGWVDSGGASAPFDSGDSAAPFGSGRPATPGAAASVAPGVKVNWWRRIDAWASP
                     SDDLVWQRAWAESPLHAGVWLAGATVVVLCFSLLTWWIGVPWVVALAVLVVPALGWLA
                     LQERTFSASRQSLLVALAVVSAVALLAALAAWRLDGRLLVMALPLLGAALVWGAALVT
                     RRTLLLPARAAA"
     gene            complement(4183..>5808)
                     /locus_tag="PLF56_06225"
     CDS             complement(4183..>5808)
                     /locus_tag="PLF56_06225"
                     /inference="COORDINATES: protein motif:HMM:NF024753.2"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="PQQ-binding-like beta-propeller repeat protein"
                     /protein_id="HOQ53195.1"
                     /translation="GVRPNPGQPPGTALTKRAVLALGDIPGYQAPTSLGNGSYGRPLL
                     GADGDITWTVYNQANQPIDNYDPAQQVAWGSGRDYTEIPGVLTFRGNNARSAPSYGTA
                     QITDKKLSVVWTHDIGAVSADGSYFPGAGWTGQPLLVKWPKATKQAMKLPAAQVDDDG
                     FVEVIYPVFDGSVYRLSLADGSVTRPKIEGKWGFKGTGSIDPRGYPLLYAGQGLNENG
                     NQKGPWEYRIFDLIQNKQVSKISGADEVSHRQDPVGWGAFDSSALIDRYSDTLIEPGE
                     NGIVYKAKLNASFDAKAGKVSVKPELTKMTYRSPVSRQYGIESSAAAWKNLMWATDND
                     GLMVCWDARTLEVVWARTTGDNADSTPVLDDSTGHPYLYTGNSVGWRGSQRRDQVTNL
                     RKIDGLTGEVVWQYDLPAYYDFHVKGGLLSTPLLGQGEVSDLVFFNVGKTTAPSEGTM
                     VALDQRTGQVVWSRSLQHYSWSSPVLIHGADGREYGVFGDSAGLLHLFDPTTGRDYTS
                     ISLGGNVEASVSAYDNMIVVASYDQKIFGIKVS"
BASE COUNT          964 a         2035 c         1953 g          856 t
ORIGIN      
        1 cgtcgacggc tccatccccg cgcagctgcg cctgctgctc gaggtggtgg cccgccggct
       61 cggcgagtcg gacgccgccg tcgtgcgcac gctgctggcc aagttcgggc tcgggcccga
      121 ccacgtcggc cggtcctgcg acagcctctc gctgggcgaa cgcacccggg cggcgctggc
      181 ggtgctgcag ggccgggccg tcaacgtgct ggtgctcgac gagcccacca accacctgga
      241 cgccgccgcg atcgagcagt tgcagcacgc gttggtggcc ttcgagggca cggtgctgat
      301 cgtgacccac gaccgggcgt tgatggacgc gctggcgccg gtgacgatcg tgccggtcaa
      361 tggccggaca tagtcggcgc tgaacgaggg cgcaaggacg atgtgagtcc gggtaccgca
      421 gacacggccc aaactcacag catcatttcg gacgtgtcac agtcaggcgg tgaccacgtc
      481 cagggtcacc tcgatgttgc ccctggtggc gttggagtag gggcacaccg cgtgcgcctg
      541 ctcggcgagc tgttcggcgc tctgctggtc gaccccgccg acctcgatca cgagagtgac
      601 cgccaacaca aagccggtgt cttcggggtg acggcccatg cccacctcgg cggtcaccgc
      661 cgcgtccgtg atcggcacgt gtgcattgcc ggccatgcgc ttcagcgcgt tgagaaagca
      721 ggcggagtaa cccgcggcga acagctgttc gggattggtc ttgccgccgg tgccaccgag
      781 ttcggtgggg gtggcgaggt ccgcctccag gatcccgtcc gatgatcgga cgtgcccgtt
      841 gcggccctcc ccggtggcgg tggcgacggc ggtgtacaga atcttcacgg ggctacctcc
      901 agcgtgcggt tactgatgca gcctaggcga ccggtcccgg gccgtggcag gagtgctgca
      961 gcgccgccga tacaatcggc cggtgaccac gccgagcgat gaatcgtccc tgcccgagca
     1021 gctgcaggtg cggcgcgaca agcgtgcccg actgctcgag gcgggcaccg atccgtaccc
     1081 gatcagcgtc ggacgcaccc actcggccgc ccaggtgcac gcccagtggg ggcacctcgc
     1141 cgccggtgag gagacccagg acgtggtcgg cgtcagcggc cgggtggtgt tcattcgcaa
     1201 caccggcaag ctgtgcttcg ccaccctgca ggacgggttc acccgcgagg attccggtac
     1261 ccggctgcag gtgatgctct cgctcgccga ggtgggcgag cagcggctgg ccgactggaa
     1321 ggccgacgtc gacctgggcg acttcgtgtt cgtgcagggt cgggtgatca gcagcaagcg
     1381 cggtgagttg tcggtactgg cgtcccaatg gcgcatggcg tcgaaggcgc tccgtccgct
     1441 gcccaccctg cacaaggatc tgggtgaaga gacccgggtg cggcagcgct acgccgacct
     1501 ggtcgcccgt ccggaggccc gcgacatggt gcgcacccgg gccacgatcg tccgttccat
     1561 cagggagacg ctgcacagcc agtgctacct cgaggtcgaa acgccgattc ttcagctggt
     1621 gcacggcggg gcggcggcgc gtccgttcca cacccacctg aacgcgttcg acattccgat
     1681 gacgctgcgc atcgcgatcg agctgtacct caagcgagcc atcgtgggcg gcgccgaccg
     1741 ggtctacgag atgggccgca tctttcgtaa cgagggcatc gacagctcgc attcggccga
     1801 gttcaccatg ctcgaggcct accagtcgtg gggcgaccag ttcaccatcg ccgcaatgac
     1861 ccgcgacctg atcgtcaacg ccgcggacgc gttgggcgtc gagtcggtgc caagccccct
     1921 cggcgaggtg cacctgaccg gtgactggca gtggctgtcg gtgtatccgg ccttgtcgga
     1981 cgcgctgggc gagcagatca ctcccgacac cccgctgcag cgactgcgag agatcgccga
     2041 cgcgcgaagc atcgagtacg acccggcctg gatcgaccag aagctggtca tggaactgtt
     2101 cgccgagatc gtcgaaccca ccctggtgca acccaccttc gtcaacggct atccggcgat
     2161 cgcgcagccg ctggcgcgca acaatccgga cgatccgcgc ctggtcgagg cctgggacct
     2221 gatcattgca ggcgtcgagc gcggcaccgg cttctccgaa ctgatcgacc ccgtcattca
     2281 gcgggacgtg ctggtggccc agtcgctgaa ggcggccgcc ggcgaccccg aagccatgca
     2341 gttggacgag gacttcctcc gcgccctcga gttcggcgcc ccgccgatgg gcggcatggg
     2401 gctgggcatc gatcgcacga tcatgctgtt caccggggcc ggcattcgcg aaacgatact
     2461 gttcccgttg ctcaagccgc acggctgagg ttctcgcgga cccgcaggga ctcgggcggc
     2521 ctggcaggga tcggtcgcag tcctgcacac gctgatccct ctgctcaagc cgctcggctg
     2581 acttctggtg gcccggcagc aggcggccgg gccaggatcg gcttgctgtc aggcacacgg
     2641 tgatccctcg ctggtcgagc aggtcgaggc ccacctgagg tggtgggcgg ggtctggaca
     2701 accgcatccg cggctcgagg gttgatcaga atcagtgatc gccttggccg acacgaaccc
     2761 tgtcggccga ggcttccgag cgatcaggcg gcggctcgcg cgggcagcag cagcgtgcga
     2821 cgggtcacca acgcggcacc ccacaccagc gccgcgccca gcagcggcag ggccatcacc
     2881 agcagccggc cgtccagtcg ccaggcggcc agcgcggcca gtagcgccac tgccgacacc
     2941 accgccagcg cgaccagcag cgactgccgc gacgccgaga acgtgcgctc ctgcaacgcc
     3001 agccagccca gtgcgggcac caccagcacc gcgagagcga ccacccaggg cacaccgatc
     3061 caccaggtca acaggctgaa gcacagcacc accacggtgg caccggccag ccacacccct
     3121 gcatgcaggg gtgactcggc ccaggcccgc tgccatacca ggtcgtcgga cgggctcgcc
     3181 cacgcgtcga tccgacgcca ccagttgacc ttgacgcccg gcgcgacgga agctgccgcg
     3241 ccgggagtgg cgggccgacc cgagccgaag ggggcggcgg agtcgccgga atcgaaggga
     3301 gcgctggcgc cacccgaatc gacccagccg gcggagccac ccgaaccgaa agctgcggcg
     3361 gagccacccg aaccggagga ggcggcggac gtatccgcag ccagggaacc atcggtacct
     3421 gctgacgagg ccatagtgcg ctcacccgcc agcccggcgc ggccctcggc ggtgaccacc
     3481 cgggcgacca gcgtgtacac cacgtcgacc gccaccacca gcagcggcgc agccaccatg
     3541 gccggcgagg cgttgccgag ccaggcgtag aacgcgaggg cgcaacccag gccggccagc
     3601 accggcccga gcgcccgatc gagaaacccg gtgggagccg gccagatcag cagcggcagc
     3661 acgcctgcga tgacgaccag aaccagcgtg cgcaggcccg ggttcggcaa ccccaccgcg
     3721 atcggcacca ggtagaccgc acccagcagc cacagcccga ccggcaaccg gcgggcgccc
     3781 gatcgttgcc cggcgccgat cgcgaagttg atcacgttgt acatcagcac gccggtcacg
     3841 gtggccatca cggcccccga ggcgaacagg tcgccgcggc gccattcgag caccagcacc
     3901 ccgaagatca ccaccccctg cacgatcaac cgccagaacc agtgcacccc gcgcgccgtg
     3961 agcaccgcgt cggcaagacc cagcagcagg gcggccggca ccgcgatcat gatcagcgtg
     4021 gtgccgccgc ccagcgccca cagcgccaac gtgcccgcca ccatgccgac cagcaccgcg
     4081 aaacccgaca tcaccgcgcc cttgcgggag gcccagatca acaccgtgga cgtgaccccg
     4141 agggccagga cgcaggcgac cacaacagcc atggagctca cctcaactga ccttgatgcc
     4201 gaagatcttc tggtcgtaac tggcgaccac gatcatgttg tcgtacgccg acaccgacgc
     4261 ttcgacgttg ccgccgagcg aaatcgaggt gtagtcgcgt ccggtggtgg gatcgaacag
     4321 gtgcagcaag cccgccgagt cgccgaacac gccgtactcg cgcccgtcgg cgccgtgaat
     4381 gagcaccggc gaactccacg agtagtgctg caggctgcgt gaccacacca cctgcccggt
     4441 gcgctggtcg agggccacca tggtgccctc ggacggtgcc gtggtcttgc ccacgttgaa
     4501 gaagaccaga tcgctcacct cgccctgccc gagcagcggc gtcgacagca gcccgccctt
     4561 gacgtgaaag tcgtagtagg caggcaggtc gtactgccag accacctcgc cggtcaaccc
     4621 gtcgatcttg cggaggttgg tgacctggtc gcgccgctgc gacccgcgcc agccgaccga
     4681 gttgcccgtg tacaggtaag ggtggccggt ggaatcgtcc agcaccggtg tcgagtcggc
     4741 gttgtcgccg gtcgttcgag cccacaccac ctcgagcgtg cgcgcgtccc agcacaccat
     4801 caggccgtca ttgtcggtgg cccacatcag gttcttccac gccgcagccg aactctcgat
     4861 gccgtactga cggctgaccg ggctgcgata ggtcatcttg gtgagctcgg gcttgaccga
     4921 caccttgccc gccttcgcgt cgaaggacgc gttgagcttg gccttgtaca cgatgccgtt
     4981 ctcacccggc tcgatcaggg tgtcggagta ccggtcgatc aaagccgagg agtcgaacgc
     5041 gccccagccg accggatcct gccggtggga gacctcgtcg gcgccgctga tcttgctgac
     5101 ctgcttgttc tggatcaggt cgaagatgcg gtactcccac ggccccttct ggttgccgtt
     5161 ctcgttgagc ccctgccccg cgtacagcag gggatagccc cgcggatcga tcgacccggt
     5221 gcccttgaag ccccatttgc cttcgatctt cggacgggtg acggacccgt cggccaggct
     5281 gagccggtac acgctgccgt cgaagaccgg gtagatcacc tcgacgaagc cgtcgtcgtc
     5341 gacctgcgcg gcgggcagtt tcatggcctg tttggtggcc ttgggccatt tgaccagcag
     5401 tggctgcccc gtccagccgg cgccgggaaa gtacgaaccg tcggcgctga ccgcaccgat
     5461 gtcgtgggtc cacaccaccg acaacttctt gtcggtgatc tgcgccgtgc cgtacgaagg
     5521 ggctgagcgc gcgttgttgc cgcggaaggt cagcactccg gggatctcgg tgtagtcgcg
     5581 acccgagccc caggccacct gttgcgcggg gtcgtagttg tcgatcggct ggttggcctg
     5641 gttgtagacc gtccaggtga tgtcaccatc ggcgcccagc agtggacgcc cgtagctgcc
     5701 gttgcccagc gaggtgggtg cttgatagcc gggaatgtcg ccgagcgcca gtacggcacg
     5761 cttggtcagg gcggtgcccg gcggctgccc cgggttcggg cggacgcc
//
LOCUS       DAOSUG010000150         5805 bp    DNA     linear   ENV 10-JUN-2024
DEFINITION  MAG TPA_asm: Micropruina sp. isolate AMR_MDS_2195
            NODE_6351_length_5805_cov_3.738435, whole genome shotgun sequence.
ACCESSION   DAOSUG010000150 DAOSUG010000000
VERSION     DAOSUG010000150.1
DBLINK      BioProject: PRJNA925477
            BioSample: SAMN32864262
            Sequence Read Archive: SRR4236673
KEYWORDS    WGS; ENV; Metagenome Assembled Genome; MAG; Third Party Data; TPA;
            TPA:assembly.
SOURCE      Micropruina sp. (wastewater metagenome)
  ORGANISM  Micropruina sp.
            Bacteria; Bacillati; Actinomycetota; Actinomycetes;
            Propionibacteriales; Nocardioidaceae; Micropruina.
REFERENCE   1  (bases 1 to 5805)
  AUTHORS   Abdulkadir,N., Saraiva,J.P., Zhang,J., Stolte,S., Gillor,O.,
            Harms,H. and Rocha,U.
  TITLE     Genome-centric analyses of 165 metagenomes show that mobile genetic
            elements are crucial for the transmission of antimicrobial
            resistance genes to pathogens in activated sludge and wastewater
  JOURNAL   Microbiol Spectr 12 (3), e0291823 (2024)
   PUBMED   38289113
REFERENCE   2  (bases 1 to 5805)
  AUTHORS   Abdulkadir,N.
  TITLE     Direct Submission
  JOURNAL   Submitted (24-MAR-2023) Environmental Microbiology, Helmholtz
            Center for Environmental Research -UFZ, Permoserstr 15, Leipzig
            04318, Germany
COMMENT     The annotation was added by the NCBI Prokaryotic Genome Annotation
            Pipeline (PGAP). Information about PGAP can be found here:
            https://www.ncbi.nlm.nih.gov/genome/annotation_prok/
            
            ##Genome-Assembly-Data-START##
            Assembly Method        :: SPAdes v. 3.11.1
            Genome Representation  :: Full
            Expected Final Version :: Yes
            Genome Coverage        :: Not Applicable
            Sequencing Technology  :: Illumina MiSeq
            ##Genome-Assembly-Data-END##
            
            ##Genome-Annotation-Data-START##
            Annotation Provider               :: NCBI
            Annotation Date                   :: 05/04/2023 04:57:10
            Annotation Pipeline               :: NCBI Prokaryotic Genome
                                                 Annotation Pipeline (PGAP)
            Annotation Method                 :: Best-placed reference protein
                                                 set; GeneMarkS-2+
            Annotation Software revision      :: 6.5
            Features Annotated                :: Gene; CDS; rRNA; tRNA; ncRNA
            Genes (total)                     :: 2,776
            CDSs (total)                      :: 2,750
            Genes (coding)                    :: 2,714
            CDSs (with protein)               :: 2,714
            Genes (RNA)                       :: 26
            tRNAs                             :: 24
            ncRNAs                            :: 2
            Pseudo Genes (total)              :: 36
            CDSs (without protein)            :: 36
            Pseudo Genes (ambiguous residues) :: 0 of 36
            Pseudo Genes (frameshifted)       :: 2 of 36
            Pseudo Genes (incomplete)         :: 32 of 36
            Pseudo Genes (internal stop)      :: 2 of 36
            ##Genome-Annotation-Data-END##
FEATURES             Location/Qualifiers
     source          1..5805
                     /organism="Micropruina sp."
                     /mol_type="genomic DNA"
                     /submitter_seqid="NODE_6351_length_5805_cov_3.738435"
                     /isolate="AMR_MDS_2195"
                     /isolation_source="Activated sludge of municipal
                     wastewater treatment plants"
                     /db_xref="taxon:2737536"
                     /environmental_sample
                     /geo_loc_name="Canada"
                     /lat_lon="49.25 N 123.1 W"
                     /collection_date="2015-06-16"
                     /metagenome_source="wastewater metagenome"
                     /note="metagenomic"
     gene            complement(<1..299)
                     /locus_tag="PLF56_06230"
     CDS             complement(<1..299)
                     /locus_tag="PLF56_06230"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_020392867.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="Asp-tRNA(Asn)/Glu-tRNA(Gln) amidotransferase
                     GatCAB subunit B"
                     /protein_id="HOQ53196.1"
                     /translation="MTLMPYDEVLDAFDPAIGLEVHVELNTVSKMFCGCSTEFGAEPN
                     THTCPVCLGLPGSLPVLNGKAVESVIRIGLALNCSIAQWCRFARKNYFYPDMPKN"
     gene            complement(296..1816)
                     /gene="gatA"
                     /locus_tag="PLF56_06235"
     CDS             complement(296..1816)
                     /gene="gatA"
                     /locus_tag="PLF56_06235"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_015070599.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_component: GO:0030956 - glutamyl-tRNA(Gln)
                     amidotransferase complex [Evidence IEA];
                     GO_function: GO:0050567 - glutaminyl-tRNA synthase
                     (glutamine-hydrolyzing) activity [Evidence IEA];
                     GO_process: GO:0006424 - glutamyl-tRNA aminoacylation
                     [Evidence IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="Asp-tRNA(Asn)/Glu-tRNA(Gln) amidotransferase
                     subunit GatA"
                     /protein_id="HOQ53197.1"
                     /translation="MSELLHASAVELGQLIASGAASSREVVQAHLDRIDAVDADVRAF
                     LHVDAERALVAADAVDSKRAAGDPLGPLAGVPIAIKDLFCMTGAPTTAGSRILEGWHP
                     PYNATVTQRVLDADLVVLGKTNLDEFAMGSSTENSGFFTTHNPWDHDRIPGGSGGGSA
                     AALAAFETPLALGTDTGGSIRQPASVTGTVGVKPTYGGTSRYGVVAMASSLDQPGPCA
                     RTVLDSALLHAVIAGWDRMDSTSIDAPVPDVVAAARQADVRGMKLGVVKEFGGEGYAP
                     GVEQRFREAVDALAERGAEIVEVSCPNFRYALGAYYLIMPSEVSSNLARFDAMRYGLR
                     VGDDGAHSAEEVMALTRDAGFGAEVKRRIIIGTYALSSGYYDAYYGSAQKARTLIARD
                     FENAFETVDVLVSPSTPTTAFAIGERTDDPLAMYAADLCTIPSNMAGNAAGSFPCGLA
                     PEDGLPVGFQVMAPVLADDRVYRVGAALERALADRWGGVLTDQLWAREAQTDGGAR"
     gene            complement(1813..2109)
                     /gene="gatC"
                     /locus_tag="PLF56_06240"
     CDS             complement(1813..2109)
                     /gene="gatC"
                     /locus_tag="PLF56_06240"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_013160978.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_component: GO:0030956 - glutamyl-tRNA(Gln)
                     amidotransferase complex [Evidence IEA];
                     GO_function: GO:0050567 - glutaminyl-tRNA synthase
                     (glutamine-hydrolyzing) activity [Evidence IEA];
                     GO_process: GO:0006424 - glutamyl-tRNA aminoacylation
                     [Evidence IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="Asp-tRNA(Asn)/Glu-tRNA(Gln) amidotransferase
                     subunit GatC"
                     /protein_id="HOQ53198.1"
                     /translation="MALTTHEVARLAALARIALSPDELAMLAPQLDVILDAVAQVTEV
                     AGDDVPPMSHALALTNVFRADEVRPSLTAQAVLAGAPSVEDERFRVPRILGDEQ"
     gene            2220..2840
                     /locus_tag="PLF56_06245"
     CDS             2220..2840
                     /locus_tag="PLF56_06245"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_015070601.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="amino acid-binding protein"
                     /protein_id="HOQ53199.1"
                     /translation="MFLLRVALPDRPGSLGAVATAMGTVGADIQAVEIVEKYEGYAVD
                     DFMVEVPHGTLPDALITACTSIGGAEVLWFSHYPVGWGLQADVAVLDDMTENPERAER
                     ILTEAAPAVFRVNWAAQVDRADGTVIARTPMAPELDRLPLDALGSLDEPHSVELPDGW
                     LAGWPELLVAVAPFKQGTALVLARAGGPAFIKSEVARLRHLAALAN"
     gene            complement(2846..3061)
                     /locus_tag="PLF56_06250"
     CDS             complement(2846..3061)
                     /locus_tag="PLF56_06250"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_015070606.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="hypothetical protein"
                     /protein_id="HOQ53200.1"
                     /translation="MEGLNSKLNLNAGAPPRVWPKLGEWGPAQVPSTRQKKRMRWFVA
                     LGFVAAAVVVFLYGWYIFTMMQSAGGG"
     gene            complement(3302..3544)
                     /locus_tag="PLF56_06255"
     CDS             complement(3302..3544)
                     /locus_tag="PLF56_06255"
                     /inference="COORDINATES: ab initio
                     prediction:GeneMarkS-2+"
                     /note="Derived by automated computational analysis using
                     gene prediction method: GeneMarkS-2+.
                     GO_function: GO:0003700 - DNA-binding transcription factor
                     activity [Evidence IEA];
                     GO_process: GO:0006352 - DNA-templated transcription
                     initiation [Evidence IEA];
                     GO_process: GO:0006355 - regulation of DNA-templated
                     transcription [Evidence IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="sigma factor"
                     /protein_id="HOQ53201.1"
                     /translation="MNAGNVLGRVQAEYWGRLPGGLIQSSGRPDLAEDALAEAFAQAA
                     RQWPAEGVPENPGGWLATVAQRRLLDTRRAEPRTWA"
     gene            complement(3541..3897)
                     /locus_tag="PLF56_06260"
     CDS             complement(3541..3897)
                     /locus_tag="PLF56_06260"
                     /inference="COORDINATES: protein motif:HMM:NF015735.2"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="YciI family protein"
                     /protein_id="HOQ53202.1"
                     /translation="MKYLVLLIGDGELRAWDSLSDEEQAAGMKKFEDFDAACAARDGV
                     EILSGEALAGPSSATTLRTRGGTLSVTEGPYAEVIEGLGGYYLIETPDLDTLIELLQV
                     LPAYDMQLQPAVDPYE"
     gene            complement(3983..>5805)
                     /gene="ligA"
                     /locus_tag="PLF56_06265"
     CDS             complement(3983..>5805)
                     /gene="ligA"
                     /locus_tag="PLF56_06265"
                     /EC_number="6.5.1.2"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_013863842.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_function: GO:0003677 - DNA binding [Evidence IEA];
                     GO_function: GO:0003911 - DNA ligase (NAD+) activity
                     [Evidence IEA];
                     GO_process: GO:0006260 - DNA replication [Evidence IEA];
                     GO_process: GO:0006281 - DNA repair [Evidence IEA]"
                     /codon_start=3
                     /transl_table=11
                     /product="NAD-dependent DNA ligase LigA"
                     /protein_id="HOQ53203.1"
                     /translation="KEDFERWAARATRETEVTAWLCELKIDGLALDLVYRDRRLVSAA
                     TRGDGRVGEDVTANVRTIAAIPGRLSDDAPDLVEARGEVFMRPEDFAVLNEGLVAAGK
                     APFANPRNSAAGSLRQKDARVTASRNLQFLCHGLGEVSQVTIDSQSHGYELLKSWGIP
                     VSSHYRVVPTLEQAWGYIDGYAEHRHGLEHEMDGVVIKVDDRQQQQRLGSTSRAPRWA
                     IAFKYPPEEVTTKLLDIRVNTGRTGRVTPYGVMTPVFVSGSTVENATLHNASEVKRKG
                     VLIGDTVVLRKAGDVIPEIVGPVVALRDGSETEFVMPTHCPSCGTLLAPEKQGDADLR
                     CPNTRSCPAQLRERLFHLASRQALDIEVLGWQAADALLSAGALTDEGDLFSLDADTLL
                     GIPMFTRGPRKGEDPSGRYLSANGEKLLANLAEAKTRPFAKFLVALSIRHIGKGVAPD
                     VAAAFPNIDALKAATDEQLAAVEGIGPTLVAAIREWFTVDWHVAIIDKWRAAGAVMAD
                     APAEASDDVPQLLAGLTVVVTGSIPGYTRDGAAEAITSRGGKSSSSVSAKTDFVVVGD
                     NAGSKYDKAVALKRPILNADGFEVLLNEGPEAAAAVAKQS"
BASE COUNT          872 a         2038 c         2010 g          885 t
ORIGIN      
        1 aagttcttcg gcatgtcggg gtagaagtag ttcttgcggg cgaaccggca ccattgcgcg
       61 atcgaacagt tgagcgccag gccgatgcgg atcaccgact cgaccgcctt accgttcaac
      121 accggcagcg agccgggtag gcccaggcag accgggcagg tgtgcgtgtt cggctcggcg
      181 ccgaactcgg tggagcagcc gcagaacatc ttcgagacgg tgttcagctc gacatgcacc
      241 tcgaggccga tcgcagggtc gaaggcgtcg agaacctcgt cgtagggcat cagggtcatc
      301 gggctccccc gtcggtctgg gcttcgcggg cccacaactg gtcggtgagc acgccgcccc
      361 agcggtcggc cagcgcgcgc tcaagcgcgg ccccgacccg gtagacgcga tcgtcggcca
      421 gcaccggcgc catcacctga aagccgacgg gcaggccgtc ttcgggggcc agcccgcacg
      481 gaaacgaccc ggccgcgtta cccgccatgt tggacggaat cgtgcacagg tcggcggcat
      541 acatggccag cggatcgtcg gtgcgctcgc cgatcgcaaa ggcggtggtg ggcgtcgacg
      601 gcgacaccag cacgtccacc gtctcgaagg cgttctcgaa gtcgcgggcg atcagcgtgc
      661 gggccttctg cgccgagccg taataggcgt cgtaataacc cgaactgagc gcgtaggtgc
      721 cgatgatgat gcgccgcttg acctcggcgc cgaagccggc gtcgcgcgtc agcgccatca
      781 cctcttcggc gctgtgcgcg ccgtcgtcgc cgacccgcag gccgtagcgc atggcgtcga
      841 agcgggccag gttcgaactc acctcactgg gcatgatcag gtagtaggcc cccagcgcgt
      901 agcgaaagtt ggggcacgac acctcgacga tctcggcgcc ccgctcggcc agggcgtcca
      961 cggcctcacg gaagcgctgc tcgacccccg gtgcgtagcc ctcgccgccg aactccttca
     1021 ccacgccgag cttcatgccg cgtacgtcgg cctgccgcgc ggcggccacc acgtcgggta
     1081 ccggagcgtc gatcgaggtc gaatccatcc gatcccagcc ggcgatcacc gcatgcagca
     1141 gagcggaatc gagcacggtg cgcgcacagg gcccgggctg gtcgagactg gacgccatcg
     1201 ccaccacccc gtagcgggac gtgccgccgt aggtcggctt cacccccacc gtgccggtca
     1261 cggacgccgg ttgacggatc gagccgccgg tgtcggtgcc cagcgccagg ggggtctcga
     1321 aggcggccag ggcggccgcc gagccgccgc cggaaccgcc ggggatgcgg tcgtggtccc
     1381 aggggttgtg ggtggtgaag aagccggagt tctcggtcga ggagcccatc gcgaactcgt
     1441 cgaggttggt cttgccgagc accaccaggt cggcgtcgag cacccgctgg gtgaccgtcg
     1501 cgttgtacgg cgggtgccag ccctccagga tgcgtgaccc ggccgtggtg ggggcgccgg
     1561 tcatgcagaa cagatccttg atggcgatcg ggacgcccgc cagtgggccc agcgggtcgc
     1621 cggcggcacg cttgctgtcc accgcgtcgg cggcgaccag ggctcgctcg gcgtccacgt
     1681 gcaggaacgc gcgcacgtct gcgtccacgg cgtcgatccg gtccaggtgc gcctggacga
     1741 cctcgcgcga actcgctgca ccgctcgcga tcaactgccc caactcgacg gccgaggcat
     1801 gaaggagttc gctcactgct cgtcccccag aatgcgcgga acgcggaacc gttcgtcctc
     1861 gacggacggc gcgcccgcca gcacggcctg cgcggtgagc gacggacgca cctcgtcggc
     1921 ccggaacacg ttggtcaagg ccaaggcgtg cgacatcggg ggcacgtcgt caccggccac
     1981 ctcggtgacc tgggccaccg cgtccagaat cacgtccagt tgaggggcca gcatcgccag
     2041 ttcgtcgggc gacaaggcga ttcttgccaa tgccgccagg cgtgccacct cgtgcgtggt
     2101 cagggccacc gctgcacctc gctctcgatc cgatcgccca tcctaaggtg tggctgtgga
     2161 tcgtcgctga cgcagcggga ttgcgaacac ctgtaacaaa tctgcgcgag actgtggcga
     2221 tgtttctgct gcgcgtcgcc ctgcccgacc gacccggttc gctgggtgcc gtggccaccg
     2281 cgatgggtac cgtgggcgcc gacatccagg ccgtcgagat cgtcgagaag tacgagggct
     2341 acgcggtcga cgacttcatg gtcgaggtgc cccacggcac cctgcccgat gccttgatca
     2401 ccgcctgcac cagcatcggc ggggccgagg tgttgtggtt ctcgcactat ccggtgggtt
     2461 gggggctgca ggccgacgtg gccgtgctgg acgacatgac cgaaaacccc gagcgggccg
     2521 aacgcattct gaccgaggcc gcgccggcgg tgtttcgggt caactgggcc gcacaggtcg
     2581 accgtgccga cggcacggtg atcgctcgca cgccgatggc gcccgagctg gaccggctgc
     2641 ccttggacgc tctcggctcg ctggacgagc cgcacagcgt cgaactgccc gacggctggt
     2701 tggccggctg gccagaactg ctggtcgcgg tcgcgccctt caagcagggc accgccctgg
     2761 tgctggcccg cgccgggggg ccggcgttca tcaagtcgga ggtggcgcga ctgcgtcacc
     2821 tggcggcgct ggcgaactga ggcgactacc ctccgccggc cgactgcatc atcgtgaaga
     2881 tgtaccagcc gtacagaaac accaccacgg cggcggcgac gaaaccgagc gcgacgaacc
     2941 agcgcatgcg cttcttctgc cgggtggacg gcacctgcgc cggcccccac tcgcccagct
     3001 tgggccacac ccggggcggc gcgcccgcgt tcaggttcag cttgctgttc aggccctcca
     3061 cgaaccgcag cctacccggc tcctgaacgt gcgccccgcc tctgtgggct gaccgaaccc
     3121 cacctcgccg gcgaggatga ccgcctgggt gcggtcctgg cgcccaactt gaccagaatg
     3181 gacgacagct agttgcagct agttgcggac cgtcttggcg gacaagagcc gaccggcgac
     3241 gagcgccaac cgcccgcccc ccaggcgatc gccagcagat agcagaggcc gcgcaggacg
     3301 atcaggccca ggttcgcggc tcggcacgcc gcgtatcgag cagccggcgc tgggcgacgg
     3361 tggcgagcca gccgcccggg ttctccggga cgccctcggc cggccactgc cgggcggcct
     3421 gggcgaaggc ttcggcaagg gcgtcctcgg ccaggtcggg gcgtccgctg gactggatca
     3481 gcccacccgg caaccgcccc cagtattcgg cctgcacccg gccgaggacg ttcccggcgt
     3541 tcactcgtag gggtcgaccg ccggctgcag ctgcatgtcg taggcgggca gcacctgcag
     3601 cagctcgatc agcgtgtcca ggtcgggggt ctcgatcagg tagtagccgc ccagcccctc
     3661 gatcacctcc gcgtagggac cctcggtgac cgacagggtg ccaccccggg tgcgcagcgt
     3721 ggtggcgctg gagggcccgg ccaacgcctc gccggacagg atctccaccc cgtcgcgcgc
     3781 ggcgcaggcg gcgtcgaagt cctcgaactt cttcatgccc gccgcctgct cctcgtcgct
     3841 gagcgaatcc caggcgcgca gttcgccgtc tccgatcaac aacaccaggt acttcatcgt
     3901 gactcctctc gacgacggga ggctcccgtc attgggttgt cgtccgggcc gcgaccgaat
     3961 cgacaaccac ccgcgacatt gctcaggatt gcttcgcgac agccgccgcg gcctccgggc
     4021 cctcgttgag cagcacctcg aagccgtcgg cgttcaggat cggacgtttc agtgcgaccg
     4081 ccttgtcgta cttgctgccg gcgttgtcgc cgacgacgac gaagtcggtc ttggccgaca
     4141 ccgaactcga ggacttgccg ccccggctgg tgatcgcctc ggcggccccg tcgcgggtgt
     4201 agccggggat cgaaccggtg accaccacgg tcagcccggc cagcagttgc ggcacgtcgt
     4261 cgctcgcctc ggcgggtgca tcggccatca cggcgccggc cgcccgccac ttgtcgatga
     4321 tggccacgtg ccagtcgacg gtgaaccatt cacggatcgc cgcgaccagg gtgggcccga
     4381 tgccctccac ggcggccagt tgctcgtcgg tggcggcctt cagcgcgtcg atgttgggga
     4441 aggccgccgc gacgtcgggc gcgacgccct tgccgatgtg ccgaatggac agcgcgacca
     4501 gaaacttcgc gaacggacgg gtcttggcct cggccaggtt ggccagcagc ttttcgccgt
     4561 tggcggacag gtagcgcccg gacggatcct cgcccttgcg cggaccgcgg gtgaacatcg
     4621 ggatccccag cagggtgtcg gcgtcgagcg agaacagatc cccctcgtcg gtcagtgctc
     4681 cggcgctcag taacgcatcg gccgcctgcc agccgagcac ctcgatgtcg agcgcctgcc
     4741 gcgaggccag gtggaacagc cgctcgcgca actgcgccgg gcacgagcgg gtgttcgggc
     4801 agcgcaggtc ggcatcgccc tgcttctccg gtgccagcag ggtgccgcag gaggggcagt
     4861 gggtgggcat cacgaactcg gtttcgctgc cgtcgcgcag cgccaccacc gggccgacga
     4921 tctcgggaat cacgtcgccg gccttgcgca gcaccacggt gtcaccgatc agtaccccct
     4981 tgcgtttcac ctcggacgcg ttgtgcaggg tcgcgttctc gaccgtcgag cccgagacga
     5041 acaccggcgt catcaccccg tacggcgtga cccggccggt gcgtccggtg ttcacgcgga
     5101 tgtcgagcag cttggtggtg acctcctccg gcgggtattt gaacgcgatc gcccagcggg
     5161 gggcccggga ggtgctgccc agccgctgtt gctgctggcg gtcgtcgacc ttgatcacca
     5221 ccccgtccat ctcgtgctcg aggccgtgcc ggtgctcggc gtagccgtcg atgtagcccc
     5281 aggcctgctc gagggtgggc accacccggt agtggctcga caccgggatg ccccacgact
     5341 tgagcaactc gtaaccgtgg gactggctgt cgatggtgac ctgcgatacc tcgcccaggc
     5401 cgtggcagag gaactgcagg ttgcgtgacg cggtgacgcg ggcgtccttc tgccgcagcg
     5461 aaccggccgc cgagttgcgc ggattcgcga agggcgcctt accggcggcg accagcccct
     5521 cgttgagcac ggcgaagtcc tcgggccgca tgaacacctc gccgcgggcc tcgaccaggt
     5581 cgggggcgtc gtcgctcagc cggccgggta tggccgcgat ggtgcggacg ttggcggtga
     5641 cgtcttcacc gacccggccg tcaccgcggg tggcggcgct gaccaggcgc cggtcgcggt
     5701 agacgaggtc gagcgccaga ccgtcgatct tgagttcgca cagccaggcg gtcacctcgg
     5761 tctcgcgagt ggcacgggcc gcccagcgct cgaagtcctc cttcg
//
LOCUS       DAOSUG010000151         5768 bp    DNA     linear   ENV 10-JUN-2024
DEFINITION  MAG TPA_asm: Micropruina sp. isolate AMR_MDS_2195
            NODE_6391_length_5768_cov_3.617889, whole genome shotgun sequence.
ACCESSION   DAOSUG010000151 DAOSUG010000000
VERSION     DAOSUG010000151.1
DBLINK      BioProject: PRJNA925477
            BioSample: SAMN32864262
            Sequence Read Archive: SRR4236673
KEYWORDS    WGS; ENV; Metagenome Assembled Genome; MAG; Third Party Data; TPA;
            TPA:assembly.
SOURCE      Micropruina sp. (wastewater metagenome)
  ORGANISM  Micropruina sp.
            Bacteria; Bacillati; Actinomycetota; Actinomycetes;
            Propionibacteriales; Nocardioidaceae; Micropruina.
REFERENCE   1  (bases 1 to 5768)
  AUTHORS   Abdulkadir,N., Saraiva,J.P., Zhang,J., Stolte,S., Gillor,O.,
            Harms,H. and Rocha,U.
  TITLE     Genome-centric analyses of 165 metagenomes show that mobile genetic
            elements are crucial for the transmission of antimicrobial
            resistance genes to pathogens in activated sludge and wastewater
  JOURNAL   Microbiol Spectr 12 (3), e0291823 (2024)
   PUBMED   38289113
REFERENCE   2  (bases 1 to 5768)
  AUTHORS   Abdulkadir,N.
  TITLE     Direct Submission
  JOURNAL   Submitted (24-MAR-2023) Environmental Microbiology, Helmholtz
            Center for Environmental Research -UFZ, Permoserstr 15, Leipzig
            04318, Germany
COMMENT     The annotation was added by the NCBI Prokaryotic Genome Annotation
            Pipeline (PGAP). Information about PGAP can be found here:
            https://www.ncbi.nlm.nih.gov/genome/annotation_prok/
            
            ##Genome-Assembly-Data-START##
            Assembly Method        :: SPAdes v. 3.11.1
            Genome Representation  :: Full
            Expected Final Version :: Yes
            Genome Coverage        :: Not Applicable
            Sequencing Technology  :: Illumina MiSeq
            ##Genome-Assembly-Data-END##
            
            ##Genome-Annotation-Data-START##
            Annotation Provider               :: NCBI
            Annotation Date                   :: 05/04/2023 04:57:10
            Annotation Pipeline               :: NCBI Prokaryotic Genome
                                                 Annotation Pipeline (PGAP)
            Annotation Method                 :: Best-placed reference protein
                                                 set; GeneMarkS-2+
            Annotation Software revision      :: 6.5
            Features Annotated                :: Gene; CDS; rRNA; tRNA; ncRNA
            Genes (total)                     :: 2,776
            CDSs (total)                      :: 2,750
            Genes (coding)                    :: 2,714
            CDSs (with protein)               :: 2,714
            Genes (RNA)                       :: 26
            tRNAs                             :: 24
            ncRNAs                            :: 2
            Pseudo Genes (total)              :: 36
            CDSs (without protein)            :: 36
            Pseudo Genes (ambiguous residues) :: 0 of 36
            Pseudo Genes (frameshifted)       :: 2 of 36
            Pseudo Genes (incomplete)         :: 32 of 36
            Pseudo Genes (internal stop)      :: 2 of 36
            ##Genome-Annotation-Data-END##
FEATURES             Location/Qualifiers
     source          1..5768
                     /organism="Micropruina sp."
                     /mol_type="genomic DNA"
                     /submitter_seqid="NODE_6391_length_5768_cov_3.617889"
                     /isolate="AMR_MDS_2195"
                     /isolation_source="Activated sludge of municipal
                     wastewater treatment plants"
                     /db_xref="taxon:2737536"
                     /environmental_sample
                     /geo_loc_name="Canada"
                     /lat_lon="49.25 N 123.1 W"
                     /collection_date="2015-06-16"
                     /metagenome_source="wastewater metagenome"
                     /note="metagenomic"
     gene            <1..756
                     /locus_tag="PLF56_06270"
     CDS             <1..756
                     /locus_tag="PLF56_06270"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_014847306.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="PTS sorbitol transporter subunit IIB"
                     /protein_id="HOQ53204.1"
                     /translation="PTVNVMPTGKTGPLAQFINESIYVSAVDVAQVKAADPAQVAAAP
                     AGRPATAADDERPKFTSDKKVSQTLAEQKNKSLLTRIGLGAGRVVAILFQAGRDGIQT
                     MIGTILPFMAFIAMLIGIITASGFGAWFTSFLTPLAGSGWGLLVLGFICSIPFLSPLL
                     APGAVIGQIIGTLIGVEIGKGSIPPSLALPALFAINTQNAADFIPVGLGLAEAEPETV
                     EVGVPAVLYSRFLNGVPRVAVAWLASIGLYSAA"
     gene            792..1160
                     /locus_tag="PLF56_06275"
     CDS             792..1160
                     /locus_tag="PLF56_06275"
                     /inference="COORDINATES: protein motif:HMM:NF015768.2"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="PTS glucitol/sorbitol transporter subunit IIA"
                     /protein_id="HOQ53205.1"
                     /translation="MTEIYRTTVHDAGPMAEAFVAEGMFVTFGSNAPEALREFCYIVD
                     INAMTAALTPGQQFTVDGRAFPITAVGDVAQRNLESLGHITVNIDGDSEPKMAGAIHI
                     RCDGPAPEITIGSVLTIEAA"
     gene            1157..1462
                     /locus_tag="PLF56_06280"
     CDS             1157..1462
                     /locus_tag="PLF56_06280"
                     /inference="COORDINATES: protein motif:HMM:NF012599.2"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_process: GO:0009401 - phosphoenolpyruvate-dependent
                     sugar phosphotransferase system [Evidence IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="HPr family phosphocarrier protein"
                     /protein_id="HOQ53206.1"
                     /translation="MITRDVIITDPDGVHALVAQELATKANSYASSLYLRHNGRTASA
                     GQPVSVLALGIRQGACITVLADGLDEAEALTALAEYLAHSAPTPPTTINTLTMEDPE"
     gene            1459..2805
                     /locus_tag="PLF56_06285"
     CDS             1459..2805
                     /locus_tag="PLF56_06285"
                     /EC_number="1.1.1.261"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_002547715.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_function: GO:0030554 - adenyl nucleotide binding
                     [Evidence IEA];
                     GO_function: GO:0046872 - metal ion binding [Evidence
                     IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="sn-glycerol-1-phosphate dehydrogenase"
                     /protein_id="HOQ53207.1"
                     /translation="MTDDLFTRGLAQAGDTKLVEVGRGVLGRTGTILRDALFADGRPA
                     LVVADERTWAAAGQAVQMALIEAGVRFLEPLIFPGYPVLYAALDTSEIIRERLRETGA
                     LGVAVGSGTINDLVKLAAGDLGQPYAVVGTAASMDGYSGFGAPMSKDGVKVTMPCPAP
                     AVVLFDLDVAAAAPPTMVASGYGDLSAKIPAGADWILADAVGLDPIDPLAWELVQSGV
                     AEALSQPKALAAGDPDAYAGLVSGLVLSGLAMQVYRGTRPASGAEHYFSHLWELDHLG
                     ADWDPPLSHGHKVAIGTLAMSAFYERFLARDLASLDIEAAVAKWAPWDAVEADIRSRF
                     TGALADHAVNETRVKYVNDIGLRHRLHTFVSNWGALKPRIVAQLVPARQLQQMLRDAG
                     APAVPEDIGLTAEAVRATFPKAMYYRSRYTVLDLAREAGWFEELVDDVFAPGGLWS"
     gene            2808..3704
                     /locus_tag="PLF56_06290"
     CDS             2808..3704
                     /locus_tag="PLF56_06290"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_016455211.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_function: GO:0016787 - hydrolase activity [Evidence
                     IEA];
                     GO_process: GO:0008152 - metabolic process [Evidence IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="HAD-IIA family hydrolase"
                     /protein_id="HOQ53208.1"
                     /translation="MTTWSKTAPLRPDFPENARPDRLFDAYVFDLDGTIYLGDHLLPG
                     VRATIELLRKRRTPVRFLSNNPTKDPHQYQAKLEALGLPTPLSDIANTIVTTVRWLTA
                     HHRDKIVFPIAEQPLITALEAAGIRMSEDPAEIDIVIASYDRTFDYRKLQIAFDALWF
                     HDRGAILVQTNPDRYCPFPGGRGEPDCAAIVAAIEACTGVTCSANFGKPDPLMVAEAL
                     EGYDVSPARTMMVGDRLATDIAMGRAAGMNTALVLSGDSTLEEATALTVKQRPHYILT
                     SLDQLIPEDVWDADGGASHIEN"
     gene            4415..5413
                     /locus_tag="PLF56_06295"
     CDS             4415..5413
                     /locus_tag="PLF56_06295"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_000082736.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_function: GO:0004803 - transposase activity [Evidence
                     IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="IS5 family transposase"
                     /protein_id="HOQ53209.1"
                     /translation="MTEFQSSLSDVEYSQRRRTPKREEFLTRMDAVVPWTDWVTLIEP
                     FYYSNKRGRRAVGLETMLRMYLLQVWFHLSDEGVEDQINDSFAMRSFMRLDFGTRQVP
                     DATTLLHFRHLLEKHKLGEAMFAALNAKLEADGLIMRGGSIVDATIIAAPSSTKNATR
                     TRDPEMHQTRKGNQWYFGMKAHAGVDAGTGLVHSVTVTAASVHDLDEIGHLVRVDDQV
                     VYADAGYQGVDKRREIVGDEHLSGVRWQVAARKGALKQMHPVEQAIESRKASVRAKVE
                     HVFLIVKRDFGFTKARYRGLAKNLNHLQIALASANLLMTARAAVIQTRLTGAVTTG"
     gene            complement(5589..>5768)
                     /locus_tag="PLF56_06300"
     CDS             complement(5589..>5768)
                     /locus_tag="PLF56_06300"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_076611738.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_process: GO:0015074 - DNA integration [Evidence IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="integrase core domain-containing protein"
                     /protein_id="HOQ53210.1"
                     /translation="RTSVFHDGPYRSIADVEYATAAWVDWYNNRRLHSSLAMVSPLEF
                     EQSHYAALNPEPQPA"
BASE COUNT         1028 a         2020 c         1786 g          934 t
ORIGIN      
        1 ccgacggtga acgtgatgcc gaccggcaag accgggcccc tggcccagtt catcaacgag
       61 agcatctacg tctccgcggt ggacgtcgcc caggtgaaag cagccgaccc ggcccaggtc
      121 gcggcggcac cggctggcag gccggcaacc gccgcggatg acgagcgccc caagttcact
      181 tccgacaaga aggtttccca gacactcgct gagcagaaga ataagtcatt gctgaccagg
      241 atcgggctgg gtgccggccg ggtcgtcgcc atcctgttcc aggccggccg tgacggcatc
      301 cagaccatga tcggcacgat ccttccgttc atggcgttca tcgcgatgct cattggcatc
      361 atcaccgcgt ccggcttcgg tgcgtggttc acctccttcc tcaccccact cgccgggtca
      421 ggttgggggc tgctcgtcct cgggttcatc tgctcgatcc cgttcctgtc cccgcttctg
      481 gcgccgggcg cggtcatcgg ccagatcatc ggcaccctga tcggtgtgga gatcggcaag
      541 ggcagcatcc cgcccagcct cgcactcccg gccctgttcg cgatcaacac ccagaacgca
      601 gccgacttca tcccggtcgg cctcggcctt gccgaagccg agcccgagac cgtcgaggtc
      661 ggcgtccccg ccgtgctgta ctcccgcttc ctcaacggtg tcccccgggt cgccgttgcg
      721 tggctggcaa gcatcggcct ctacagcgcc gcctgagcag cgctctctga gcaatacgaa
      781 ggagttaggt gatgactgag atctatcgga ccacagtgca cgacgcagga cccatggctg
      841 aggccttcgt agcggagggg atgttcgtca ccttcggctc caacgccccc gaagccctcc
      901 gcgagttctg ctacatcgtc gacatcaacg cgatgacggc agcactcacc ccgggccagc
      961 aattcaccgt cgatggtcgg gccttcccga tcaccgcggt gggcgacgtg gcgcaacgca
     1021 acctcgaatc cctgggtcac atcaccgtca acatcgacgg tgactccgaa cccaagatgg
     1081 ccggagcgat ccacatccgc tgcgacggac cagcccccga gatcacgatc gggagcgtgc
     1141 tcacgatcga ggcggcgtga tcactcgcga cgtcatcatc accgatccgg atggtgtcca
     1201 cgccctcgtg gcgcaggaac tggcaaccaa ggcgaacagc tacgccagta gcctctacct
     1261 gcgccacaac gggcgaacgg cgagtgcggg gcaacccgtg tccgtgctcg ccctcggaat
     1321 ccggcaaggt gcctgcatca ccgtgctcgc agacggcctc gacgaagccg aggctctcac
     1381 cgcgcttgcc gagtatctag ctcactcggc gccaacccca cccaccacga tcaacaccct
     1441 caccatggag gacccagaat gaccgacgac ctcttcacac gtggcctggc ccaggccggc
     1501 gacaccaagc tggtggaagt gggccgagga gtgctcggcc gaactggaac catcctgcgt
     1561 gacgccctgt tcgccgatgg tcgaccagca ctggtggtcg ccgacgaacg cacgtgggca
     1621 gccgccggcc aggcagtcca gatggcactc atcgaagctg gagtgcggtt cctcgagccg
     1681 ctcatcttcc cgggctaccc ggtcctctac gcggcgctcg acaccagcga gatcattcgt
     1741 gaacgactcc gggagaccgg cgcactcggc gtcgccgtcg gctccggcac catcaacgac
     1801 ctcgtcaagc ttgccgccgg agacctcggc cagccctacg ccgtcgtcgg cacggccgcc
     1861 tccatggatg gctactccgg cttcggcgca ccgatgagca aggacggggt gaaggtcacc
     1921 atgccctgcc cggcaccggc ggtcgtcctc ttcgacctcg acgtggccgc ggccgctccg
     1981 cccaccatgg tggcatccgg ctacggcgac ctgtcggcca agatcccggc cggcgcagac
     2041 tggatcctcg ccgacgccgt cgggctggac ccgatagacc cactcgcctg ggaactcgtg
     2101 cagagcgggg tcgccgaggc cctctcccag ccgaaagccc tcgcagccgg cgaccccgac
     2161 gcctacgccg gcctggtctc cggcctggtg ctctccggcc tcgccatgca ggtgtaccgc
     2221 ggcacccgcc ccgcctcggg cgccgagcac tacttcagcc acctgtggga actcgaccac
     2281 ctcggagccg actgggaccc gcccttgtct catggacaca aggtcgccat cggcaccctc
     2341 gcgatgagtg ccttctacga acggttcctc gcccgtgacc tcgcctccct cgacatcgag
     2401 gccgccgtcg ccaagtgggc cccctgggat gccgttgagg cggacatccg cagccggttc
     2461 accggtgcac tcgccgacca cgcggtcaac gagacaaggg tcaagtacgt caacgacatc
     2521 ggactgcggc accgactcca cacgttcgtg tcgaactggg gcgccctcaa acctcggatc
     2581 gtcgcccagc tggtgccggc caggcagttg cagcagatgc tccgcgatgc cggcgcgcct
     2641 gccgtcccgg aggacatcgg cctgaccgct gaggccgtgc gcgccacctt cccgaaagcc
     2701 atgtactacc gatcccgcta caccgtcctc gacctcgccc gagaggccgg ctggttcgag
     2761 gagctggtcg acgatgtctt cgcccccggc ggactgtgga gctgaccatg accacctgga
     2821 gcaagaccgc acccctgagg cccgacttcc cggagaacgc ccggcccgac cggctcttcg
     2881 acgcctacgt cttcgacctc gacggcacca tctacctcgg cgaccacctc ttgccggggg
     2941 tgagggcgac aatcgaactg ctgcgcaagc ggcgtacacc ggtccggttc ctttcgaaca
     3001 accccaccaa ggacccccac cagtaccagg ccaagctcga ggcgctcggc ctcccgaccc
     3061 cgctgagcga catcgcgaat acgatcgtca ccacggtccg ttggctcacc gcccaccacc
     3121 gcgacaagat cgtgttcccg atcgccgaac agccgctgat cacagccctg gaagccgccg
     3181 ggatcaggat gagtgaggac cctgccgaga tcgacatagt gatcgcgtcc tacgaccgta
     3241 cgttcgacta ccgcaagctc cagatcgcct tcgacgccct ctggttccac gaccggggag
     3301 cgatcctcgt gcagaccaac cccgaccgct actgcccctt ccccggcgga cgcggcgagc
     3361 ccgactgcgc cgccatcgtc gccgccatcg aggcctgcac cggcgtcacc tgctcagcca
     3421 acttcggcaa gcccgacccc ctcatggtcg ccgaagccct cgaaggctac gacgtctccc
     3481 ccgcccgcac gatgatggtc ggcgaccggc tcgccacaga catcgccatg ggaagagccg
     3541 cgggcatgaa caccgccctc gtcctctccg gcgacagcac cctggaggaa gccacagccc
     3601 tcaccgtcaa acagcgcccc cactacatcc ttacgagcct cgaccagctc attccagagg
     3661 acgtatggga cgcggacggc ggtgcctccc acatcgagaa ctagcatgcc cgtccgcgat
     3721 caagccggct caggtcttct ttccagcggg gtggggcgga agcggatctg acgttcggcc
     3781 ccagtggctg gtccatccga gcatgaaacc agtggcagca tcctgcagaa cctccgtgcg
     3841 tgcgtcccgg acgcgtcgag gtgggtcggc gtcgaaccac tcgatgactt ccccgcgcgc
     3901 atcctcgagt gacacttccg gcatccagct tcgctgacag ggcggcgtcg tcggaactcc
     3961 tggggttgct gctctgattc gcaccgacga cggtcgccag gaggcgacgg cgggcgatac
     4021 tccgcgccct gggtaaggca gaagccatct acgcggagcc cagcttctcg gtccaggagg
     4081 ctggcgtcca atgttcagtt cgacgttgga gagcgccgtc cagcgctatc cagcagcgtc
     4141 gtcatcgaag tggttggtct tgaccagaat ccatgcccgg tggcaccccg ggctgtggac
     4201 gtcaacggtg tggtggaggc cgggggcctc ggcggattgg gacttgccgg gtgcttggac
     4261 atgacacaaa gaaatcgccg ctctgtgcga ggcgacccgg ccagctggtc tagggaaatg
     4321 ctgattaagg agcgagtctt ggcggtcggg gtgcggctgc tcgatggggt gtcgtggggc
     4381 ctgaatcgta cggtggctag gtaaaatggt ggtcatgacc gagttccagt ccagcctgtc
     4441 tgatgttgag tacagccagc ggcgtcggac cccgaaacgg gaagagttcc tgacccggat
     4501 ggacgccgtg gtgccgtgga cggactgggt cacgctgatc gagccgttct actactccaa
     4561 caagcgggga cgccgtgcgg tgggcttgga gacgatgctg cggatgtacc tgttgcaggt
     4621 gtggttccac ctgtccgatg agggcgtgga agaccagatc aacgactcgt tcgcgatgcg
     4681 gtcgttcatg cggctcgact tcggcacccg gcaggtgccg gacgcgacca ccctgctgca
     4741 tttccggcat ctgttggaga agcacaagtt gggcgaggcc atgttcgcgg cgttgaacgc
     4801 gaagctggag gctgacggtc tgatcatgcg tgggggcagc atcgtggatg ccacgatcat
     4861 cgccgcccca agttcgacca agaacgcgac ccgaacacgc gacccggaga tgcatcagac
     4921 caggaagggc aaccagtggt acttcgggat gaaggcccac gccggggtgg atgccggcac
     4981 cggcttggtc cacagcgtca cggtcaccgc agccagcgtg catgacctgg acgagatcgg
     5041 ccatctggtc cgtgtcgacg atcaggtggt gtacgccgat gccggctacc aaggggtcga
     5101 caagcgtcgc gagatcgtcg gcgatgagca cctgtcagga gtgaggtggc aggtcgcggc
     5161 acgcaagggt gcgttgaagc agatgcaccc tgtcgagcag gcgatcgagt cccgcaaggc
     5221 gtcggtgcgg gccaaagtcg agcacgtgtt cttgatcgtg aagcgggact tcgggttcac
     5281 caaggcccgc taccgcgggc tggccaagaa cctcaaccac ctccagatcg ccctcgcctc
     5341 agcgaaccta ctcatgaccg cccgagccgc cgtgatccaa accagactga ccggcgcggt
     5401 cacgaccggg taaccccggc ggcagcctgc ccaaaacccg ccagtcagcc ccgaaccagg
     5461 ggcacgaaga agaaccagac aggccacgac cctcaccaag cggtcccaag cccgcccagc
     5521 gacgccccaa ccccagtagc ctcaatgatc agcgtttccc ttgaactgcc ccaggttttg
     5581 tgccgctcct atgcgggttg cggctctggg ttcagagctg cgtagtggga ctgttcgaac
     5641 tctagcgggc tgaccattgc gaggctgctg tggaggcgtc ggttgttgta ccagtcgacc
     5701 caggcggcgg tggcgtactc gacgtcggcg atgctgcggt atggcccgtc gtggaacacg
     5761 ctggtgcg
//
LOCUS       DAOSUG010000152         5737 bp    DNA     linear   ENV 10-JUN-2024
DEFINITION  MAG TPA_asm: Micropruina sp. isolate AMR_MDS_2195
            NODE_6420_length_5737_cov_3.780183, whole genome shotgun sequence.
ACCESSION   DAOSUG010000152 DAOSUG010000000
VERSION     DAOSUG010000152.1
DBLINK      BioProject: PRJNA925477
            BioSample: SAMN32864262
            Sequence Read Archive: SRR4236673
KEYWORDS    WGS; ENV; Metagenome Assembled Genome; MAG; Third Party Data; TPA;
            TPA:assembly.
SOURCE      Micropruina sp. (wastewater metagenome)
  ORGANISM  Micropruina sp.
            Bacteria; Bacillati; Actinomycetota; Actinomycetes;
            Propionibacteriales; Nocardioidaceae; Micropruina.
REFERENCE   1  (bases 1 to 5737)
  AUTHORS   Abdulkadir,N., Saraiva,J.P., Zhang,J., Stolte,S., Gillor,O.,
            Harms,H. and Rocha,U.
  TITLE     Genome-centric analyses of 165 metagenomes show that mobile genetic
            elements are crucial for the transmission of antimicrobial
            resistance genes to pathogens in activated sludge and wastewater
  JOURNAL   Microbiol Spectr 12 (3), e0291823 (2024)
   PUBMED   38289113
REFERENCE   2  (bases 1 to 5737)
  AUTHORS   Abdulkadir,N.
  TITLE     Direct Submission
  JOURNAL   Submitted (24-MAR-2023) Environmental Microbiology, Helmholtz
            Center for Environmental Research -UFZ, Permoserstr 15, Leipzig
            04318, Germany
COMMENT     The annotation was added by the NCBI Prokaryotic Genome Annotation
            Pipeline (PGAP). Information about PGAP can be found here:
            https://www.ncbi.nlm.nih.gov/genome/annotation_prok/
            
            ##Genome-Assembly-Data-START##
            Assembly Method        :: SPAdes v. 3.11.1
            Genome Representation  :: Full
            Expected Final Version :: Yes
            Genome Coverage        :: Not Applicable
            Sequencing Technology  :: Illumina MiSeq
            ##Genome-Assembly-Data-END##
            
            ##Genome-Annotation-Data-START##
            Annotation Provider               :: NCBI
            Annotation Date                   :: 05/04/2023 04:57:10
            Annotation Pipeline               :: NCBI Prokaryotic Genome
                                                 Annotation Pipeline (PGAP)
            Annotation Method                 :: Best-placed reference protein
                                                 set; GeneMarkS-2+
            Annotation Software revision      :: 6.5
            Features Annotated                :: Gene; CDS; rRNA; tRNA; ncRNA
            Genes (total)                     :: 2,776
            CDSs (total)                      :: 2,750
            Genes (coding)                    :: 2,714
            CDSs (with protein)               :: 2,714
            Genes (RNA)                       :: 26
            tRNAs                             :: 24
            ncRNAs                            :: 2
            Pseudo Genes (total)              :: 36
            CDSs (without protein)            :: 36
            Pseudo Genes (ambiguous residues) :: 0 of 36
            Pseudo Genes (frameshifted)       :: 2 of 36
            Pseudo Genes (incomplete)         :: 32 of 36
            Pseudo Genes (internal stop)      :: 2 of 36
            ##Genome-Annotation-Data-END##
FEATURES             Location/Qualifiers
     source          1..5737
                     /organism="Micropruina sp."
                     /mol_type="genomic DNA"
                     /submitter_seqid="NODE_6420_length_5737_cov_3.780183"
                     /isolate="AMR_MDS_2195"
                     /isolation_source="Activated sludge of municipal
                     wastewater treatment plants"
                     /db_xref="taxon:2737536"
                     /environmental_sample
                     /geo_loc_name="Canada"
                     /lat_lon="49.25 N 123.1 W"
                     /collection_date="2015-06-16"
                     /metagenome_source="wastewater metagenome"
                     /note="metagenomic"
     gene            complement(<1..504)
                     /locus_tag="PLF56_06305"
     CDS             complement(<1..504)
                     /locus_tag="PLF56_06305"
                     /inference="COORDINATES: protein motif:HMM:NF040015.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="DUF6177 family protein"
                     /protein_id="HOQ53211.1"
                     /translation="MRIVHPLADEWTDEYAVYVSDALQVLLTDPLQSFLQWTLSEGLR
                     PVLVTGEAARLSPFVSLAMNRAGGFWAVRSDQGVYDGLTGFPISGFADLWTPRAAEGQ
                     RPARFLTPSDDPRGLLAYDIFGHQRAVADTQVGRLAQTVHQGLGGAELDVWGLAEPLV
                     EPFGIDPL"
     gene            complement(507..803)
                     /locus_tag="PLF56_06310"
     CDS             complement(507..803)
                     /locus_tag="PLF56_06310"
                     /inference="COORDINATES: ab initio
                     prediction:GeneMarkS-2+"
                     /note="Derived by automated computational analysis using
                     gene prediction method: GeneMarkS-2+."
                     /codon_start=1
                     /transl_table=11
                     /product="hypothetical protein"
                     /protein_id="HOQ53212.1"
                     /translation="MISDTNAYDVGASQQVQDDFDTAAGNLEAAILAHDGDVNNAMAE
                     YQAEDVSEGYQAMEKQWKDAGDEVKQIIKTLRESLSKNDDIAIQTMAKAKSYLP"
     gene            complement(819..1163)
                     /locus_tag="PLF56_06315"
     CDS             complement(819..1163)
                     /locus_tag="PLF56_06315"
                     /inference="COORDINATES: ab initio
                     prediction:GeneMarkS-2+"
                     /note="Derived by automated computational analysis using
                     gene prediction method: GeneMarkS-2+."
                     /codon_start=1
                     /transl_table=11
                     /product="hypothetical protein"
                     /protein_id="HOQ53213.1"
                     /translation="MKFSVGTQTLGTLLNRTTTQSDDLKTLVEKFIAAAEPLQNRMSG
                     PAKDAFDKFKGRSDQVAGTLHNALVGICGSIDGQNKSFIQGAEEGADTHSQSEGSANF
                     AGVDAVARFGPR"
     gene            1327..2133
                     /locus_tag="PLF56_06320"
     CDS             1327..2133
                     /locus_tag="PLF56_06320"
                     /inference="COORDINATES: protein motif:HMM:NF012708.2"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_function: GO:0005515 - protein binding [Evidence IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="FHA domain-containing protein"
                     /protein_id="HOQ53214.1"
                     /translation="MSIAHTSPRPLTRDAAPSPQVVPARARGLLATVLDVALALAPLL
                     VAWPLGHTRLPAWAVWTITLTLVVAVAAALLAELTRTGCTPARRLLDVRTVAAASALP
                     PTLREVVRGRVGTADLRAGRDPLRLVPAGDRPLEPAGWTLWQQSNIVGTGPWLLLLDN
                     GEQVPLDGSTLVGRDPANEPGQSHALVAVPDLTRSISRVHALLEPDADCVWLTDTGAT
                     NGTRAATPSASGGTVIERWLRPGERVALQAGATVHLGDRRLQVTRSNRAA"
     gene            2133..2639
                     /locus_tag="PLF56_06325"
     CDS             2133..2639
                     /locus_tag="PLF56_06325"
                     /inference="COORDINATES: protein motif:HMM:NF037633.2"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="hypothetical protein"
                     /protein_id="HOQ53215.1"
                     /translation="MPASAQVLIETTRTHRNRLGSALAYGELEERRTPNTNRLRVLGS
                     IVVAAVICAVLVGTSFVMNLLSTQKEEQAVAAFRTALAANPLKPGGDWVQDEPTGFLR
                     NKRTGVLVDPQTGYLVDRRTGLSKDPRGRLIDPRIDWYIDPETGYYTDPATGLTIDPR
                     TQQIVRTR"
     gene            2636..3934
                     /locus_tag="PLF56_06330"
     CDS             2636..3934
                     /locus_tag="PLF56_06330"
                     /inference="COORDINATES: protein motif:HMM:NF020396.2"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="EsaB/YukD family protein"
                     /protein_id="HOQ53216.1"
                     /translation="MSDYTRLTVQGTLNRADLVLPSDEPLAGLLPEMLTLLHEPAEQA
                     RPLSLVTVLGEQLVTSLSLAEQNVRQGSIVRLVRVDEAPPPPEVADITQLAGESVAAR
                     ADRWSRTWGVLAAAGAALVAGWLIGGQLPTTGVGTTWLLGAAVALAAVAIGLARRGGG
                     GAAAVVAALAAGLIGESMQALVHDQPWGAGWGAWLGACAALVTVVALFGYRERGLALG
                     AGAGAALVCIWAGLGLSGMTPLNSAGVVALAGALALGVLPGLAMTTSGLAGLDDRVIE
                     GAAVRRAEAEQAVEATHRGLNWTCLTIAAGTAAAGLVLAGSSDPWALGLAGAVATVAA
                     LRTRVLPLAPQRLALFAAAIAIGGGLAATGLHASPTWTLTALVALAAALVVLTGLRLS
                     DNVIARLGRLGNLIEMLAVLAGVPLLLGLLGVFRDLLAAF"
     gene            3934..4746
                     /locus_tag="PLF56_06335"
     CDS             3934..4746
                     /locus_tag="PLF56_06335"
                     /inference="COORDINATES: ab initio
                     prediction:GeneMarkS-2+"
                     /note="Derived by automated computational analysis using
                     gene prediction method: GeneMarkS-2+."
                     /codon_start=1
                     /transl_table=11
                     /product="hypothetical protein"
                     /protein_id="HOQ53217.1"
                     /translation="MPRLRLTWPGAVLRASVPGEPQRLAALDAAIRAPLPLSTGVGVL
                     GAGPDAPTALVAALVAGVLAARRPHRVLAVGASAAERGIAWHAGLGSSARASDDAVSR
                     RGAANSGAEATEGLARTPGDLFALDLGARPERWWQAVAPITRFFDFVVTDWGTPSDPD
                     DARAAGAVLLVVTDARRASLQAGVDLAHRLTTEHAAPLLVVHDTATGSDHGLRAAARV
                     NGARWLPADPALRASVPVPVRRLRYAGTRAVLELTADVVDRAVARNLAEATA"
     gene            4743..>5737
                     /locus_tag="PLF56_06340"
     CDS             4743..>5737
                     /locus_tag="PLF56_06340"
                     /inference="COORDINATES: ab initio
                     prediction:GeneMarkS-2+"
                     /note="Derived by automated computational analysis using
                     gene prediction method: GeneMarkS-2+."
                     /codon_start=1
                     /transl_table=11
                     /product="cell division protein FtsK"
                     /protein_id="HOQ53218.1"
                     /translation="MTVLVHRPTRATQPVPPPPPETLAPPPPLITEAGGFPLQFLLPM
                     VGAMSSVVMMVVMRNGQPLFLLIAGVIFVVAIVAGLGFALSSRGRKAAEQALQRTRYL
                     DYLEKLRRDLQQRSDQVRDAAALLHPAPDALLALVRDPSRLWERRRRDQDFLQVRVGI
                     GAVPWFGLSVPAAESPVQPQDPYLLAEATAVVDRFGTVPAMPVTIDLRTAHEVAVIGD
                     RDTVLPMVRALVLQLAAVHSPDDLQLALACPAERLADWAGFDLLPQVQHAELFDGPVP
                     ARRIAESMGALGQVLGPDLTDRLQQAHTTRRTGAATTPPPRLLVISDGHGQRAAGL"
BASE COUNT          705 a         2072 c         2102 g          858 t
ORIGIN      
        1 gagcgggtcg atgccgaacg gctcgaccaa cggctcggcc aacccccaca cgtccagctc
       61 ggcaccgccc aggccctggt gcacggtctg cgccaaccgg cccacctgcg tgtcggcgac
      121 cgcccgctgg tggccgaaga tgtcgtaggc cagcaggccc cgcggatcat ccgacggggt
      181 cagaaagcgt gccggccgct gcccctcggc ggcgcgcggc gtccacaggt cggcaaagcc
      241 gctgatcgga aagccggtca ggccgtcgta gacaccctgg tcggaccgga ccgcccagaa
      301 gccaccggcc cggttcatcg ccagcgagac gaacgggctg agccgcgccg cttcgccggt
      361 gaccagcacc ggacgcaggc cctcgctgag cgtccactga agaaacgact gcagcgggtc
      421 ggtgagcagc acctgcaaag catccgagac gtagaccgca tactcgtcgg tccactcgtc
      481 ggcaagaggg tgaacgatgc gcatgatcag ggcaggtacg acttggcctt ggccatggtc
      541 tggatcgcga tgtcgtcgtt cttgctcagc gattcgcgca gcgtcttgat gatctgcttc
      601 acctcgtcgc cggcgtcctt ccactgcttc tccatggcct ggtagccctc gctgacgtcc
      661 tcggcctggt actcggccat cgcgttgttg acgtcgccgt cgtgagcgag gatcgcggcc
      721 tcgagattgc ccgcggcagt gtcgaaatcg tcctgcacct gctgcgaggc gccgacgtca
      781 taggcattgg tatcggagat catcggggat cagtcctttc agcgcgggcc gaagcgggcg
      841 acggcgtcga ccccggcgaa gttggccgag ccttcgcttt ggctgtgggt gtcggcgccc
      901 tcttcggcgc cctggatgaa cgacttgttc tgcccgtcga tcgagccaca gatgccgacc
      961 agcgcgttgt gcagcgtgcc ggccacctgg tcgcttctgc ccttgaactt gtcgaaggcg
     1021 tccttggcgg ggccgctcat gcggttctgc agcggctcgg cggcggcgat gaacttttcc
     1081 accagcgtct tcagatcgtc ggattgggtg gtggtgcggt tgagcagggt gcccaaagtc
     1141 tgcgtgccca cgctgaactt catgatcgag tcccatcctg gttgggtcgt ggattgccgg
     1201 tcagccttca cggtaggtgg gtgagggccg ttgcacgatg gggagcactc cccaccgacc
     1261 cggcacaccg taggccgtga acgggtcacg ggttagggtg aatgaaaccc cgatccgaag
     1321 gcaccgatga gcattgccca cacctcgccg cgcccgctga cgcgcgacgc cgcgccgtcc
     1381 ccgcaggtcg tgccggcgcg tgcgcggggg ctgctcgcca ccgttctgga cgtggcgctg
     1441 gcactggctc cgctgcttgt ggcgtggccg ttggggcaca cccggctgcc cgcctgggcg
     1501 gtctggacga tcacgctgac gctggtcgtg gcggtggccg ccgcgctgct ggccgagctg
     1561 acccgcaccg gctgcacccc ggcccgacgg ctgctcgacg tgcgcaccgt ggcggcggcc
     1621 tccgcgctgc cgccgacgct gcgcgaggtc gtccgtggcc gggtcggtac cgccgacctg
     1681 cgcgccggac gggatccgtt gcggctggtg ccggcgggcg atcgtccgct cgaaccggcc
     1741 ggctggacgc tgtggcagca gtcgaacatc gtcggcaccg gcccgtggct gctgctgctc
     1801 gacaatggcg aacaggtgcc gctggacggc tctaccctgg tgggccgcga ccccgccaac
     1861 gagccgggcc agagtcatgc cctggtggcg gtgcccgatc tgacccgcag catctcgcgg
     1921 gtgcacgcac tgctcgagcc cgatgccgac tgcgtgtggc tgaccgacac cggcgccacc
     1981 aacggcaccc gggccgccac cccgtccgcc tcggggggaa ccgtgatcga gcgctggctg
     2041 cgccccggcg aacgggtcgc gctgcaggcc ggagccaccg tgcacctggg cgatcgccgg
     2101 ctgcaggtca cccgctcgaa tcgggcggcc tgatgcccgc gtccgcacag gtgctgatcg
     2161 agaccacccg cacccaccgc aaccggctcg gctcggcgct ggcctacggc gaactcgaag
     2221 agcgccgcac ccccaacacc aacaggttgc gggtgctcgg ctcgatcgtg gtggccgcgg
     2281 tgatctgcgc ggtgctggtc ggcaccagct tcgtgatgaa cctgctgtcc acgcagaagg
     2341 aggaacaggc ggtggcggcg ttccgcacgg cgctggcggc caatccactc aagccgggcg
     2401 gcgactgggt gcaggacgag ccgaccggct ttctgcgcaa caagcgcacc ggcgtgctgg
     2461 tcgacccgca gaccggttat ctggtcgatc ggcgcaccgg gttgtcgaaa gacccgcgcg
     2521 gccggctgat cgatccccgc atcgactggt acatcgaccc tgagaccggc tactacaccg
     2581 atccggcaac cggattgacg atcgacccgc gaacccagca gatcgtgcgt acccggtgag
     2641 cgactacacc cgcctcaccg tgcagggcac cctcaaccgg gccgacctgg tgctgccctc
     2701 ggacgaaccg ctggcggggc tgctgcccga gatgctcacc ctgctgcacg aaccggcgga
     2761 gcaggctcgt ccgctgagcc tggtgacggt gctcggcgag caactggtca cctcgttgag
     2821 cctggccgag cagaacgtgc ggcagggcag catcgtccgg ctggttcggg tcgacgaggc
     2881 accgcccccg cccgaggtgg ccgacatcac gcaactggcc ggcgagtcgg tcgcggcccg
     2941 tgccgaccgg tggagccgga cgtggggggt gctggccgcg gccggcgccg cactggtggc
     3001 cggctggctg atcggcgggc aactgccgac caccggcgtc ggcaccacct ggctgctggg
     3061 tgctgcggtc gcgctggcgg cggtcgcgat cgggctggcc cggcgtggcg gtggcggcgc
     3121 cgccgcggtg gtcgccgccc tggccgccgg cctgatcggc gagtccatgc aggcgctcgt
     3181 gcacgaccag ccctggggcg ccgggtgggg tgcctggctc ggtgcctgcg ccgcgctggt
     3241 gacggtggtg gcgctgttcg gctaccgcga gcggggcctg gcgctgggcg ccggggcggg
     3301 tgcagcactg gtctgcatct gggccggtct cgggctgagt ggcatgacgc cactgaactc
     3361 ggccggcgtg gtggccctgg cgggcgcgct cgcgctcggc gtgctgcccg gcctggcgat
     3421 gaccacctcg ggtctcgccg gcctggacga ccgggtgatc gaaggcgccg cagtccgccg
     3481 ggccgaggcc gagcaggccg tcgaggccac ccaccgcggc ctgaactgga cctgcctcac
     3541 gatcgccgcc ggcaccgcgg cggccggcct ggtgctggcc ggttcgagcg acccctgggc
     3601 gctgggattg gctggcgcgg tggccacggt ggcggccctg cgcacccggg tgctgccgtt
     3661 ggcgccgcag cggctggcgc tgttcgcggc cgccatcgcg atcggcggcg ggctcgcggc
     3721 gaccggcctg cacgcctcgc cgacctggac gctgaccgcc ctggtcgcgc tcgccgccgc
     3781 gctggtggtg ctgaccgggc tgcggctcag cgacaacgtg atcgcccggc tcggccggct
     3841 gggcaacctg atcgagatgc tcgcggtact ggcgggtgtg ccgctgctgc tgggcctgct
     3901 gggcgtgttc cgcgacctgt tggcggcgtt ctgatgccgc ggctacggct gacgtggccg
     3961 ggcgcggtgc tgcgggcctc ggtgccgggc gagccccagc ggctggccgc gctcgatgcg
     4021 gcgatccggg caccgttgcc gttgagcacc ggcgtgggcg tgctcggtgc cggcccggac
     4081 gccccgaccg cgctggtcgc cgcgctggtg gcgggggtgc tggccgcccg gcgtccgcac
     4141 cgggtgttgg cggtcggggc ctcggcggcg gagcggggca tcgcctggca cgccggtctg
     4201 ggttcgtccg cccgggcctc ggacgacgcg gtgagccggc gcggcgccgc caactcgggg
     4261 gccgaggcga ccgagggcct ggcccgtacg ccaggggacc tgttcgcgct cgacctgggt
     4321 gcgcgtcctg agcgctggtg gcaggcggtg gccccgatca cccgcttctt cgacttcgtg
     4381 gtgaccgact ggggcacccc gagcgatccg gacgatgccc gcgccgccgg cgccgtgctg
     4441 ctggtggtga cggacgcccg gcgcgcctca ctgcaggccg gcgtcgacct ggcccatcgg
     4501 ctgaccaccg agcacgccgc tcccctgctg gtggtgcacg acaccgctac cggcagcgat
     4561 cacggcctgc gcgcggcggc ccgggtcaac ggcgcccgct ggctgcccgc cgaccccgcg
     4621 ctgcgtgcat ccgtgccggt gccggtgcgc cgcctgcgct acgccggcac ccgggccgtg
     4681 ctcgaactga ccgccgacgt ggtcgaccgt gcggtcgccc gcaacctcgc ggaggcgacc
     4741 gcatgaccgt gctcgtgcac cgacccaccc gcgccaccca accggtgccg cccccgccgc
     4801 ccgagacgtt ggccccgccg ccaccgctga tcactgaggc gggcggcttt ccgctgcagt
     4861 tcctgctgcc gatggtcggg gcgatgtcgt cggtggtgat gatggtggtg atgcgcaacg
     4921 ggcagccgtt gttcctgctg atcgccgggg tgatcttcgt ggtcgcaatc gtcgccgggc
     4981 tgggcttcgc gctcagttcg cgcggccgca aggccgccga gcaggccctc caacgcaccc
     5041 gctacctcga ctacctggag aagctgcgcc gcgacctgca gcagcgcagc gatcaggtgc
     5101 gcgatgccgc cgccctgctg caccccgcac ccgacgccct gctggcgctg gtccgcgacc
     5161 cgagccggct gtgggagcgg cgccgtcgcg accaggactt tctgcaggtc cgggtgggca
     5221 tcggcgcggt gccgtggttc gggctgagcg tgccggccgc cgaatcgccg gtgcagccgc
     5281 aggaccccta cctgctggcc gaggcgaccg cggtggtcga ccggttcggc accgtgccgg
     5341 cgatgccggt gacgatcgac ctgcgtaccg cccacgaggt ggcggtgatc ggcgatcgcg
     5401 acaccgtcct gcccatggtg cgggccctgg tgctgcaact ggcggccgtc cactcgccgg
     5461 atgatctgca gctcgccctg gcgtgcccgg cggaacggct ggccgactgg gccggattcg
     5521 acctgctgcc ccaggtgcag cacgccgagc tgttcgacgg accggtaccc gcccgccgca
     5581 tcgccgaatc gatgggcgcg ctcggccagg tgctcggccc ggacctgacg gaccggctgc
     5641 agcaggcaca caccacccgg cgcaccggcg ccgcgaccac cccgccgccg cggctgctgg
     5701 tgatcagcga cggccacggc caacgagccg ccggcct
//
LOCUS       DAOSUG010000153         5722 bp    DNA     linear   ENV 10-JUN-2024
DEFINITION  MAG TPA_asm: Micropruina sp. isolate AMR_MDS_2195
            NODE_6434_length_5722_cov_4.114346, whole genome shotgun sequence.
ACCESSION   DAOSUG010000153 DAOSUG010000000
VERSION     DAOSUG010000153.1
DBLINK      BioProject: PRJNA925477
            BioSample: SAMN32864262
            Sequence Read Archive: SRR4236673
KEYWORDS    WGS; ENV; Metagenome Assembled Genome; MAG; Third Party Data; TPA;
            TPA:assembly.
SOURCE      Micropruina sp. (wastewater metagenome)
  ORGANISM  Micropruina sp.
            Bacteria; Bacillati; Actinomycetota; Actinomycetes;
            Propionibacteriales; Nocardioidaceae; Micropruina.
REFERENCE   1  (bases 1 to 5722)
  AUTHORS   Abdulkadir,N., Saraiva,J.P., Zhang,J., Stolte,S., Gillor,O.,
            Harms,H. and Rocha,U.
  TITLE     Genome-centric analyses of 165 metagenomes show that mobile genetic
            elements are crucial for the transmission of antimicrobial
            resistance genes to pathogens in activated sludge and wastewater
  JOURNAL   Microbiol Spectr 12 (3), e0291823 (2024)
   PUBMED   38289113
REFERENCE   2  (bases 1 to 5722)
  AUTHORS   Abdulkadir,N.
  TITLE     Direct Submission
  JOURNAL   Submitted (24-MAR-2023) Environmental Microbiology, Helmholtz
            Center for Environmental Research -UFZ, Permoserstr 15, Leipzig
            04318, Germany
COMMENT     The annotation was added by the NCBI Prokaryotic Genome Annotation
            Pipeline (PGAP). Information about PGAP can be found here:
            https://www.ncbi.nlm.nih.gov/genome/annotation_prok/
            
            ##Genome-Assembly-Data-START##
            Assembly Method        :: SPAdes v. 3.11.1
            Genome Representation  :: Full
            Expected Final Version :: Yes
            Genome Coverage        :: Not Applicable
            Sequencing Technology  :: Illumina MiSeq
            ##Genome-Assembly-Data-END##
            
            ##Genome-Annotation-Data-START##
            Annotation Provider               :: NCBI
            Annotation Date                   :: 05/04/2023 04:57:10
            Annotation Pipeline               :: NCBI Prokaryotic Genome
                                                 Annotation Pipeline (PGAP)
            Annotation Method                 :: Best-placed reference protein
                                                 set; GeneMarkS-2+
            Annotation Software revision      :: 6.5
            Features Annotated                :: Gene; CDS; rRNA; tRNA; ncRNA
            Genes (total)                     :: 2,776
            CDSs (total)                      :: 2,750
            Genes (coding)                    :: 2,714
            CDSs (with protein)               :: 2,714
            Genes (RNA)                       :: 26
            tRNAs                             :: 24
            ncRNAs                            :: 2
            Pseudo Genes (total)              :: 36
            CDSs (without protein)            :: 36
            Pseudo Genes (ambiguous residues) :: 0 of 36
            Pseudo Genes (frameshifted)       :: 2 of 36
            Pseudo Genes (incomplete)         :: 32 of 36
            Pseudo Genes (internal stop)      :: 2 of 36
            ##Genome-Annotation-Data-END##
FEATURES             Location/Qualifiers
     source          1..5722
                     /organism="Micropruina sp."
                     /mol_type="genomic DNA"
                     /submitter_seqid="NODE_6434_length_5722_cov_4.114346"
                     /isolate="AMR_MDS_2195"
                     /isolation_source="Activated sludge of municipal
                     wastewater treatment plants"
                     /db_xref="taxon:2737536"
                     /environmental_sample
                     /geo_loc_name="Canada"
                     /lat_lon="49.25 N 123.1 W"
                     /collection_date="2015-06-16"
                     /metagenome_source="wastewater metagenome"
                     /note="metagenomic"
     gene            complement(<1..315)
                     /locus_tag="PLF56_06345"
     CDS             complement(<1..315)
                     /locus_tag="PLF56_06345"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_011183879.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_component: GO:0016020 - membrane [Evidence IEA];
                     GO_function: GO:0016765 - transferase activity,
                     transferring alkyl or aryl (other than methyl) groups
                     [Evidence IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="UbiA family prenyltransferase"
                     /protein_id="HOQ53219.1"
                     /translation="MHQAVSTVDAEASGSLRAYLALTKPRIIELLLVTTVPAMFLAAG
                     GVPQWQLVIWTLLGGTLAAGSANVFNSVLDADIDSHMRRTRRRPMVQQSVPAGRAYVF
                     GAV"
     gene            438..1529
                     /gene="tal"
                     /locus_tag="PLF56_06350"
     CDS             438..1529
                     /gene="tal"
                     /locus_tag="PLF56_06350"
                     /EC_number="2.2.1.2"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_008357144.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_function: GO:0004801 - transaldolase activity [Evidence
                     IEA];
                     GO_process: GO:0006098 - pentose-phosphate shunt [Evidence
                     IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="transaldolase"
                     /protein_id="HOQ53220.1"
                     /translation="MNDRLKALADAGVSIWLDDLSRARISSGNLAAKIADDSVSGVTT
                     NPTIFAAALTNGADYAEQLRSLGDEIDVDHAIKQLTSTDVRNACDLFAQVYSSSNGYD
                     GRVSIEVAPTLANNTGETIAQASELYDLVDRPNVLIKIPATKPGLPAIKETIAAGISV
                     NVTLIFSEERYREVMDAYLSGLEAADAAGKNLATIHSVASFFVSRVDAEIDKRLAAAG
                     RDDLKSKAAIANALVAYGAFEEVFGSERFAKLKAKGANVQRPLWASTGTKDPKLPDTL
                     YVTNLIADGVVNTMPEKTMDAFGDHGELGEPINGKAAQGRAVLDEVAAAGVDLAEVFD
                     ALEAEGVEKFIVSWHELIASVEKAIASVK"
     gene            1630..3090
                     /gene="zwf"
                     /locus_tag="PLF56_06355"
     CDS             1630..3090
                     /gene="zwf"
                     /locus_tag="PLF56_06355"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_016665798.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_function: GO:0004345 - glucose-6-phosphate
                     dehydrogenase activity [Evidence IEA];
                     GO_process: GO:0006098 - pentose-phosphate shunt [Evidence
                     IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="glucose-6-phosphate dehydrogenase"
                     /protein_id="HOQ53221.1"
                     /translation="MVLFGVTGDLARKKLMPAIYDLANRGLLPPGFGLIGFARRDWEH
                     QDFAKIVHDSVKERARTPFREEVWKQLLEGIRFVPGTFDSDESFETLKTTLTELDEVQ
                     GTGGNHAFYLSIPPKAFDQVISMLKKHGLAKRDQKGSWSRVVIEKPFGHDLASAVELE
                     RRVSSVFHSQSVFRIDHYLGKETVQNLLALRFANELFEPIWNRNYVDHVQITMAEDIG
                     IGGRAGYYDGVGAARDVIQNHLLQLLALTAMEEPTSFEADQLRAEKRKVLAAAKAPQD
                     IAAHTARGQYLAGWAGGKQVMGYLEEEGISPQSQTETYAAIRVDIDNRRWAGVPFYLR
                     SAKRMPRRVTEIALNLRRAPHLPFSQTDTEALGSNALLLRIQPDEGITLRFGAKVPAT
                     LMEIRDVTMDFAYGTSFAESSPEAYERLILDVLLGDPPLFPQHEEVELSWKILDPILD
                     FWADLDTQPEGYPSGSWGPSSGNTMLARDGFVWRRP"
     gene            3106..4035
                     /locus_tag="PLF56_06360"
     CDS             3106..4035
                     /locus_tag="PLF56_06360"
                     /inference="COORDINATES: protein motif:HMM:NF021632.2"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="glucose-6-phosphate dehydrogenase assembly
                     protein OpcA"
                     /protein_id="HOQ53222.1"
                     /translation="MIISIPDTNATGIHMALVQARHSIGVASSMVFTMIVIADLRHYD
                     KALEACRDAGREHPSRIILVTDGQAKADRVDAEIRLGEEVPGEIILLRFHGELANHRD
                     SVVLPLLLPDSPVVAWWPAGAPDKPGQDPLGKLAIRRITDAMGSSHPLAAIERRARNY
                     SPGDTDLTWTRLTPWRALLAASVDAYPSKVLGAKVEGAPDNAGSLLLAAWLEDRLGMP
                     VTRSEAGRGIGITDVTLHTEKGDITITREDGKMATFSAPGLNARRVALRRRDINALIT
                     EELRRLDVDDIFASAMTTLAKGCERRDAEKASR"
     gene            4032..4763
                     /gene="pgl"
                     /locus_tag="PLF56_06365"
     CDS             4032..4763
                     /gene="pgl"
                     /locus_tag="PLF56_06365"
                     /EC_number="3.1.1.31"
                     /inference="COORDINATES: protein motif:HMM:TIGR01198.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_function: GO:0017057 - 6-phosphogluconolactonase
                     activity [Evidence IEA];
                     GO_process: GO:0006098 - pentose-phosphate shunt [Evidence
                     IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="6-phosphogluconolactonase"
                     /protein_id="HOQ53223.1"
                     /translation="MNDQVLRFADSDALVDRAAGMLAAQIAGYQTDPDRIVHLCLTGG
                     RIAQRLYDSFGGRLEAAGADPGRMELWWTDERFTSTDDPARHAGPALAMLAGKFQLDP
                     ARTHPMPAADGVADADAAATTYAKELGDVVFDICLLSIGPEGHVAGIFPGHPSFDTHA
                     QTVIGLTDSPKGEGDRISLTAAALKRSHEVWFLANGDDKAAAVAGALAGSSDTPAGLV
                     RGRHATKWFIDRDAARDLPWFECDW"
     gene            complement(5328..5684)
                     /locus_tag="PLF56_06370"
     CDS             complement(5328..5684)
                     /locus_tag="PLF56_06370"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_007433696.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="RNA polymerase-binding protein RbpA"
                     /protein_id="HOQ53224.1"
                     /translation="MVGGSAIRGSRVGAGPMGEAERGDSAPRLYVSYFCSNRHETRPA
                     FAADAVVPETWDCPRCGLPANLASENPPPPPKIQPYKTHLAYVKERRSDAEAAAILTE
                     ALEGLRARRAAGEVIY"
BASE COUNT         1026 a         1945 c         1872 g          879 t
ORIGIN      
        1 caccgcaccg aagacgtagg cgcggccggc cggcacgctc tgctgcacca tcggacgacg
       61 ccgcgtgcgg cgcatgtgac tgtcgatgtc ggcgtccagc accgagttga acacgttggc
      121 cgacccggcg gccaacgtgc cgcccagcag cgtccagatc accaactgcc actgcggcac
      181 cccgccggcg gcgagaaaca tggcgggaac ggtggtgacc agcagcaact cgatgatgcg
      241 cggcttggtc agcgccagat aggcacgcag ggaccccgaa gcctcggcgt caaccgtcga
      301 aaccgcctgg tgcacgtcca ccctcttccg tcatgggtcg aatcgcgagc agtctagccg
      361 cagacgcccg tgccgcatac gagccccact agagtgactg cgtacgtctg agaatcatcg
      421 cgaggagtgg atcgagaatg aatgatcggt tgaaggcact ggctgacgcc ggggtctcca
      481 tctggctcga cgacctgtcg cgagcccgga tcagcagcgg caacctggcc gcgaagatcg
      541 ccgacgactc ggtgtcgggt gtgaccacga acccgaccat cttcgccgcg gcactgacca
      601 acggcgccga ctatgccgag cagttgaggt cgctgggcga cgagatcgac gtcgaccacg
      661 cgatcaagca actcacctcg accgatgtgc gcaacgcctg cgacctgttc gcgcaggtgt
      721 acagcagttc gaacggctac gacggccggg tctcgatcga ggtcgccccg accctcgcga
      781 acaacaccgg cgagaccatc gcccaggcgt ccgagctgta cgacctggtc gaccgaccga
      841 acgtgctgat caagattccg gccaccaagc ccggcctgcc cgccatcaaa gagaccatcg
      901 cggcaggcat ttcggtcaac gtcaccctga tcttctccga agagcgctac cgcgaggtca
      961 tggacgccta cctctcgggc ctcgaagccg ccgacgccgc gggcaagaac ctggcgacga
     1021 tccactcggt cgcgtcgttc ttcgtcagtc gggtcgacgc cgagatcgac aagcgactcg
     1081 ccgccgccgg tcgcgacgac ctgaagtcga aggcggcgat cgcgaacgcg ctggtcgcct
     1141 acggcgcgtt cgaagaggtg ttcggttccg agcggttcgc caagttgaag gcgaagggtg
     1201 ccaacgtgca gcgtccgctg tgggcttcta ccggcaccaa ggaccccaag ctgcccgaca
     1261 ccctgtacgt caccaacctg atcgcggacg gtgtggtcaa caccatgccc gagaagacca
     1321 tggacgcctt cggcgatcac ggcgagctgg gcgagccgat caacggcaag gcggcgcagg
     1381 gccgggcggt gctcgacgag gtggccgccg caggcgtcga cctggccgag gtcttcgacg
     1441 cgctcgaagc cgagggcgtc gagaagttca tcgtctcgtg gcacgagctc atcgcgtcgg
     1501 ttgagaaggc aattgcctcc gtcaagtgaa gtcggcaacg atagcggggt gacttcccgc
     1561 aagcagttcg tcaacccgct gcgcgatccc caggatcggc ggttgcctcg cgtcgccggg
     1621 ccgtgtgccc tggtgctgtt cggggtcacc ggcgacctgg cccgcaagaa actgatgccg
     1681 gcaatctacg atctggccaa ccgtggcctg ctgcccccgg gtttcgggct gatcggtttc
     1741 gcacggcgtg actgggagca ccaggacttc gccaagatcg tccacgactc ggtgaaagag
     1801 cgggcccgta caccctttcg cgaagaggtc tggaagcagc ttctcgaggg catccggttc
     1861 gtgccgggca ccttcgacag cgacgagtcg ttcgaaacgc tgaagacgac cctgaccgag
     1921 ctggacgagg tgcagggcac gggcggcaac cacgccttct acctgtcgat tcccccgaag
     1981 gcgttcgacc aggtgatctc gatgctgaag aagcacggtc tggccaagcg cgatcagaag
     2041 ggctcctgga gccgggtggt gatcgagaag ccgttcggcc acgacctggc cagcgctgtc
     2101 gagctcgaac gccgggtgag ttcggtgttc cactcccagt cggtgttccg catcgatcac
     2161 tacctcggca aagaaacggt gcagaacctg ctggcgctgc gcttcgcgaa cgagctgttc
     2221 gagccgatct ggaaccgcaa ctacgtcgat cacgtacaga tcaccatggc cgaagacatc
     2281 ggcatcggtg gccgggccgg ctactacgac ggtgtcggcg cggcgcgcga cgtgattcag
     2341 aaccacctgc tgcagctgtt ggcgttgacc gcgatggaag agccgacgtc gttcgaggcc
     2401 gaccagctgc gggccgagaa gcgcaaggtg ctggcggcgg ccaaggcgcc ccaagacatt
     2461 gcggcgcaca ccgcccgggg ccaatacctc gcgggctggg cgggtggcaa acaggtgatg
     2521 ggctatctcg aagaagaagg catcagcccg cagtcgcaga ccgagaccta cgccgcgatc
     2581 cgggtcgaca tcgacaaccg acgctgggcg ggggtgccgt tctacctgcg gtcggccaaa
     2641 cgcatgccgc gccgggtcac cgagatcgcg ctcaacctgc gccgcgcccc ccacctgccg
     2701 ttcagccaga ccgacaccga ggcgctgggc agcaacgctt tgctgctgcg cattcagccc
     2761 gacgagggca tcacgttgcg gttcggcgcc aaggtgccgg ccaccctgat ggagatccgc
     2821 gacgtcacca tggacttcgc ctacggcacc tcgttcgccg aatcgagccc ggaggcgtac
     2881 gagcggttga tcctggacgt gctgctcggc gatccgccgc tgttcccgca gcacgaagag
     2941 gtggagttgt cgtggaagat cctcgacccg atcctcgact tctgggccga cctcgacacc
     3001 cagcccgagg ggtacccctc cggatcgtgg ggtccatcga gcggcaacac catgcttgcc
     3061 cgcgacggct tcgtctggcg caggccctag cgggaggcac ccaccatgat catcagcatt
     3121 cccgacacca atgccaccgg catccacatg gcactggtgc aggcccggca cagcatcggc
     3181 gtggcctcgt ccatggtgtt cacgatgatc gtgatcgccg atctgcggca ctacgacaag
     3241 gcccttgagg cgtgccgcga cgctggccgc gagcacccgt cccgcatcat tctggtcacg
     3301 gacggccagg cgaaggccga tcgggtggac gccgagatcc ggctgggtga agaggttccg
     3361 ggcgagatca tcttgctgcg gttccacggc gagttggcca accaccgcga ctcggtggtg
     3421 ctgccgctat tgctgcccga ctcccccgtc gtggcctggt ggccggccgg cgcacccgac
     3481 aagccgggcc aggacccgct gggcaagctg gcgatccggc ggatcaccga cgccatgggg
     3541 tcgtcccatc cgctggccgc gatcgaacgc cgggcccgca actactcccc cggcgacacc
     3601 gacttgacct ggacccggct caccccgtgg cgggcccttc tggccgcctc cgtcgacgcc
     3661 tacccgtcga aggtgctggg cgccaaggtc gagggtgctc ccgacaatgc aggcagcctg
     3721 ctgctggcgg cctggctgga ggaccgcctc ggcatgccgg tcacccgttc cgaggcaggg
     3781 cgcggcatcg gcatcaccga tgtcacgctg cacaccgaga agggtgacat cacgatcacg
     3841 cgcgaggacg gcaagatggc caccttctcg gcgcccggcc tgaacgcgcg gcgcgtcgcc
     3901 ctgcgccggc gcgacatcaa cgcgctgatc accgaagaac tgcgacgcct cgacgtcgac
     3961 gacatcttcg cctcggcgat gaccaccctg gcgaaaggct gcgaacggcg cgatgccgaa
     4021 aaggcgtcgc ggtgaacgat caggtcctgc ggttcgccga ctcggacgct ctggtcgacc
     4081 gcgccgcggg catgctggcg gcgcagatcg ccggctacca gaccgacccc gaccgcatcg
     4141 tccatctctg tctgacgggc ggacgcatcg cccagcgcct gtatgacagc ttcggcggcc
     4201 ggctcgaagc cgccggagcc gacccgggcc gcatggagtt gtggtggacc gacgagaggt
     4261 tcacctcgac cgacgatccg gcccggcacg cggggcctgc actggccatg ctggccggca
     4321 agtttcagct cgatcccgct cgcacacacc cgatgccggc ggccgacggg gtcgcggacg
     4381 cggacgccgc cgcgaccacg tatgccaagg agctcggcga cgtggtgttc gacatctgcc
     4441 tgctgagcat cggccccgag gggcatgtgg cgggcatctt tccgggacac ccgtccttcg
     4501 atacccatgc ccagaccgtg atcgggctga ccgattcacc caagggcgag ggcgatcgca
     4561 tctcgcttac cgcggcggca ctcaaacgct cgcatgaggt gtggtttctg gccaacggcg
     4621 acgacaaggc tgccgcagtg gccggcgcgc ttgcgggttc gtccgacacg ccggccggcc
     4681 tggttcgcgg acgtcatgcc accaagtggt tcatcgaccg cgacgctgct cgcgacctgc
     4741 cctggttcga gtgcgactgg tagccgatgg gcgacaccag tacgaggcca agagcaacca
     4801 ggttcatcgc ccccgcgctt gaccggagtc ccccgtgcat tcgggacgcc ccctcaggta
     4861 ctgcccatca ccgccgcctc cgcccagtct cgacaacacg caaccatgga ctgatctcga
     4921 acgggctcca ccgacgggct ccctacatct gcagagcctc caaaggctgg ccgctcgctg
     4981 ccaggactgg ccacgataaa tggtcgccgg cctgcagagg agccggaacg aaattcgccc
     5041 cgtgaagata taccgatttt accgccagat tgcagatgga acgattcaaa gcgtcatttg
     5101 gcaaatctgg cggtaaaatc ggtatatcga tgggtggtga aaagttagct ggcgcatgtg
     5161 gttatcgctg gaccgacgag atcccggcgt tcaccgggat gacgggaagg ccttcgccag
     5221 catgaccgga gacgggcgcc ggcatgaccg gacaacgtcc gcccggatga tgggacagcc
     5281 ttcgcgagac cccgttcgcc gggaggacgg tgatgaccga cgaacggtca gtagatgacc
     5341 tcgccggcgg cacggcgggc acgcagtccc tcgagcgcct cggtgaggat cgcggccgcc
     5401 tcggcgtcgc tgcgccgctc cttcacatac gcgaggtgcg tcttgtacgg ctggatcttc
     5461 ggtggcggcg gcgggttctc ggaggcgagg ttggccggca agccgcagcg cgggcagtcc
     5521 caggtttcgg gcaccacagc gtccgcggcg aacgccggac gggtctcatg ccggttcgag
     5581 caaaagtacg agacgtacaa ccggggagcg gaatcgcccc gctcggcttc gcccatcgga
     5641 ccggccccga cccggctgcc ccgaatcgcg ctaccaccaa ccacgtgtgt ttctccttgt
     5701 cagatcgccc agaaatgggc gg
//
LOCUS       DAOSUG010000154         5617 bp    DNA     linear   ENV 10-JUN-2024
DEFINITION  MAG TPA_asm: Micropruina sp. isolate AMR_MDS_2195
            NODE_6544_length_5617_cov_5.381158, whole genome shotgun sequence.
ACCESSION   DAOSUG010000154 DAOSUG010000000
VERSION     DAOSUG010000154.1
DBLINK      BioProject: PRJNA925477
            BioSample: SAMN32864262
            Sequence Read Archive: SRR4236673
KEYWORDS    WGS; ENV; Metagenome Assembled Genome; MAG; Third Party Data; TPA;
            TPA:assembly.
SOURCE      Micropruina sp. (wastewater metagenome)
  ORGANISM  Micropruina sp.
            Bacteria; Bacillati; Actinomycetota; Actinomycetes;
            Propionibacteriales; Nocardioidaceae; Micropruina.
REFERENCE   1  (bases 1 to 5617)
  AUTHORS   Abdulkadir,N., Saraiva,J.P., Zhang,J., Stolte,S., Gillor,O.,
            Harms,H. and Rocha,U.
  TITLE     Genome-centric analyses of 165 metagenomes show that mobile genetic
            elements are crucial for the transmission of antimicrobial
            resistance genes to pathogens in activated sludge and wastewater
  JOURNAL   Microbiol Spectr 12 (3), e0291823 (2024)
   PUBMED   38289113
REFERENCE   2  (bases 1 to 5617)
  AUTHORS   Abdulkadir,N.
  TITLE     Direct Submission
  JOURNAL   Submitted (24-MAR-2023) Environmental Microbiology, Helmholtz
            Center for Environmental Research -UFZ, Permoserstr 15, Leipzig
            04318, Germany
COMMENT     The annotation was added by the NCBI Prokaryotic Genome Annotation
            Pipeline (PGAP). Information about PGAP can be found here:
            https://www.ncbi.nlm.nih.gov/genome/annotation_prok/
            
            ##Genome-Assembly-Data-START##
            Assembly Method        :: SPAdes v. 3.11.1
            Genome Representation  :: Full
            Expected Final Version :: Yes
            Genome Coverage        :: Not Applicable
            Sequencing Technology  :: Illumina MiSeq
            ##Genome-Assembly-Data-END##
            
            ##Genome-Annotation-Data-START##
            Annotation Provider               :: NCBI
            Annotation Date                   :: 05/04/2023 04:57:10
            Annotation Pipeline               :: NCBI Prokaryotic Genome
                                                 Annotation Pipeline (PGAP)
            Annotation Method                 :: Best-placed reference protein
                                                 set; GeneMarkS-2+
            Annotation Software revision      :: 6.5
            Features Annotated                :: Gene; CDS; rRNA; tRNA; ncRNA
            Genes (total)                     :: 2,776
            CDSs (total)                      :: 2,750
            Genes (coding)                    :: 2,714
            CDSs (with protein)               :: 2,714
            Genes (RNA)                       :: 26
            tRNAs                             :: 24
            ncRNAs                            :: 2
            Pseudo Genes (total)              :: 36
            CDSs (without protein)            :: 36
            Pseudo Genes (ambiguous residues) :: 0 of 36
            Pseudo Genes (frameshifted)       :: 2 of 36
            Pseudo Genes (incomplete)         :: 32 of 36
            Pseudo Genes (internal stop)      :: 2 of 36
            ##Genome-Annotation-Data-END##
FEATURES             Location/Qualifiers
     source          1..5617
                     /organism="Micropruina sp."
                     /mol_type="genomic DNA"
                     /submitter_seqid="NODE_6544_length_5617_cov_5.381158"
                     /isolate="AMR_MDS_2195"
                     /isolation_source="Activated sludge of municipal
                     wastewater treatment plants"
                     /db_xref="taxon:2737536"
                     /environmental_sample
                     /geo_loc_name="Canada"
                     /lat_lon="49.25 N 123.1 W"
                     /collection_date="2015-06-16"
                     /metagenome_source="wastewater metagenome"
                     /note="metagenomic"
     gene            <1..2429
                     /locus_tag="PLF56_06375"
     CDS             <1..2429
                     /locus_tag="PLF56_06375"
                     /inference="COORDINATES: protein motif:HMM:NF028442.2"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=3
                     /transl_table=11
                     /product="glycosyl hydrolase"
                     /protein_id="HOQ53225.1"
                     /translation="AALGLETAGFVQGVVAYRCTANCADGATETPVLDAASAVNLTRA
                     VSGDTVTWTAPAGTDRYLLVASWMNGTGQTIDLAATPTPSFMVDHFSSAGASAITSYW
                     ERNVLTPQLRAAFRKSGGSLFFDSLELNHQDKQIRHWTDDFLSQFQKRRGYSLVPYLA
                     AVATSTPQFDFAGGVGGRVRQDYTQTLSELFVAHHIEPLKAWAHRYGLMMRGQAYSSW
                     GPTPLDYTDAATALDIPEQEANNRSTATNPLFGTAGSDTWRQVVSANAMAGRSIVSWE
                     AGTFGREDGLARQSLVAKLNSQFSLGVNKVIYHGWADQSPGTATTWPGYYPFRNYVGD
                     VYGPQTPFSVDDTTINDYVGRVQRVLRRGTLASDVAVFWDRAGDAQFDDTTLADAGYS
                     YGFVNNTLVTKASSRVRNDRVTRLGYRAIVVDALGDGANLDLATAKRLLGWARQGLPI
                     VVVGDPSDQVNGYHPGQDAAMARTIERLLAARSVVTVSSRTKALGALRSLGVNPDASY
                     DSEPLRTLHRTTKDSDYYFLFNSSTERTSTTVTLKGRGVPYRYNAWTGAVTPIAEYSR
                     TRSGVRVPVDLATGQGELIALTRGNADTPRTRCTPSALSTTADELVVGSGSSLLMRDA
                     KAGRYSTRLADGRRVISTIDTVAAPVTPQSWTLNVTSWHAGASPSETAKTALDEIMLT
                     TGSDKTLPSWQKIDGLANSSGTAVYSTKLDVGPAWTGGTGAYLDLGQVLGTAQVSVNG
                     RQLPALDQVGTGRIDLGGYLNAGSNTIEVRIATPVNNAGFGTKDAYGLVGPVTLTPYG
                     QASIQVACRR"
     gene            complement(2522..3688)
                     /gene="rhaI"
                     /locus_tag="PLF56_06380"
     CDS             complement(2522..3688)
                     /gene="rhaI"
                     /locus_tag="PLF56_06380"
                     /EC_number="5.3.1.14"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_020391687.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_function: GO:0008740 - L-rhamnose isomerase activity
                     [Evidence IEA];
                     GO_process: GO:0019299 - rhamnose metabolic process
                     [Evidence IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="L-rhamnose isomerase"
                     /protein_id="HOQ53226.1"
                     /translation="MTIDQATLDTLATLAIEVPSWAYGNSGTRFKVFTLPGGPRDPYE
                     KIDDAAQVNALTGLAPAVSLHIPWDKVDDWDALGAHAADRGVRIGTINSNVFQDDDYR
                     LGSLCNPDPRIRRKAIDHHLECIEIMHATGVQDLKIWLADGTNYPGQDDLRARQDRLA
                     QGLREIYDGLGEKQRLVLEYKFFEPAFYHMDVPDWGTSLTHCLALGEKASVCLDTGHH
                     APGTNIEFIVTQLLRHGRLGSFDFNSRFYADDDLIVGSADPFQLFRILYEVVRGGGLD
                     PASGLVFMLDQCHNIEPKIPGQIRSVLNVQEMLARALLIDRDALSAAQQAGDVLGANQ
                     VYMDAFYTDVRADLRQWRESRGLPADPMAAYAASGYAATIEADRVGGAQASWGA"
     gene            3859..4875
                     /locus_tag="PLF56_06385"
     CDS             3859..4875
                     /locus_tag="PLF56_06385"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_012917936.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_function: GO:0000976 - transcription cis-regulatory
                     region binding [Evidence IEA];
                     GO_function: GO:0003700 - DNA-binding transcription factor
                     activity [Evidence IEA];
                     GO_function: GO:0043565 - sequence-specific DNA binding
                     [Evidence IEA];
                     GO_process: GO:0006351 - DNA-templated transcription
                     [Evidence IEA];
                     GO_process: GO:0006355 - regulation of DNA-templated
                     transcription [Evidence IEA];
                     GO_process: GO:0045892 - negative regulation of
                     DNA-templated transcription [Evidence IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="LacI family DNA-binding transcriptional
                     regulator"
                     /protein_id="HOQ53227.1"
                     /translation="MAASVKDVARHAGLSVGTVSNVLNRPDSVTEATKAKVMAAIDEL
                     GYVRNEAARQLRAGMSHCVGVIVLNSSNPFFNDVAAGAEAWAADAKVSVLVGNSGELV
                     EREKAYLDLFEEQRVRGVLLSPVGQVTKRLQRMRKGGIATVLVDRDIAGDFSSVSVDD
                     RLGGRLAMEHLISTGRRRIAFVGGPLSLGQVADRFAGAAEVAGEHADVFLEFVATDRL
                     TLAHGRVAGERLLARPAGTRPDAVFAANDLLALGLLQAIASTRRLRVPDDIALIGYDD
                     IDFAAGAAVPLSSIAQPRFELGRLAMDLLLKAADDPSRPVERVVLQPFLVARASTQST
                     SSTR"
     gene            4980..>5617
                     /locus_tag="PLF56_06390"
     CDS             4980..>5617
                     /locus_tag="PLF56_06390"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_008363920.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_function: GO:0005524 - ATP binding [Evidence IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="ATP-binding cassette domain-containing protein"
                     /protein_id="HOQ53228.1"
                     /translation="MDPSQQTPVLELKGVSKSFGAVVALRNADLALYAGSIHALVGEN
                     GAGKSTVVKIMAGLYQRDSGTFAMDGEPVHFRNTAEAKAAGIAVIYQEPTLFPDLSVT
                     ENVFMGKELAASHPNIKLVDTVYGNDDDQVSFDKTSGLLSAHPNLKGIISPTTVGIAA
                     AARYLSKSDAKGKVALTGLGTPNQLREYVKDGTVKEFALWNPQDLGTLATYAA"
BASE COUNT          937 a         1957 c         1861 g          862 t
ORIGIN      
        1 gcgcagccct gggcctcgaa actgccggtt tcgtgcaggg cgtcgtcgcc taccgctgca
       61 ccgcgaactg cgcggacggc gccaccgaga cacccgttct ggacgccgcc agcgcggtca
      121 acctcaccag agccgtctcg ggcgacacgg tgacctggac ggcccccgcg ggcaccgacc
      181 gctacctgct ggtcgcctca tggatgaacg ggacggggca gaccatcgac ctggccgcga
      241 cgcccacccc cagcttcatg gtggaccact tcagctcggc cggcgcgtcc gcgatcacct
      301 cctactggga gcgcaacgta ctcacgcccc aactgcgggc ggcgttccgc aagagcggcg
      361 gctcgctgtt cttcgactcg ctggaactga accatcagga caagcagatc cggcactgga
      421 ccgacgactt cctgagccag ttccagaagc gtcgcgggta ctcgctggtg ccctacctgg
      481 ccgcggtcgc gacgtccacc ccgcagttcg acttcgccgg cggtgtcggc gggcgtgtcc
      541 gccaggacta cacccagacg ctttcggagc tgttcgtcgc ccaccacatc gagccgctga
      601 aggcttgggc gcaccgctac ggactgatga tgcgcgggca ggcgtacagc agttggggtc
      661 cgaccccgct ggactacacc gacgccgcga ccgcgctcga cattcccgag caggaggcga
      721 acaaccgctc caccgccacc aacccgctgt tcggcaccgc gggctcggac acttggcggc
      781 aggtggtcag cgccaacgcc atggcgggcc gcagcatcgt gtcgtgggag gccggaacct
      841 tcggtcgtga ggacggcctg gcccggcagt cgctggtcgc caagctcaac agccagttct
      901 cactcggggt caacaaggtg atctaccacg gttgggccga tcagtcgccg ggaacggcga
      961 cgacgtggcc gggctactac ccgttccgca actacgtcgg cgacgtgtac ggcccccaga
     1021 cccccttctc ggtcgacgac accacgatca acgactacgt cggacgcgtg cagcgcgtgc
     1081 tgcgtcgcgg cacgctggcc tcagacgtgg ccgtcttctg ggaccgcgcc ggcgacgctc
     1141 agttcgacga caccacactg gcggacgcgg ggtactccta cggcttcgtc aacaacaccc
     1201 tggtcaccaa ggcgagctcg cgggtgagga acgatcgcgt gacgcgcctg ggttaccggg
     1261 cgatcgtcgt cgacgcactc ggcgacgggg cgaacctcga cctcgcgacc gccaagcggc
     1321 tgctgggctg ggcccggcag ggcctgccga tcgtcgtggt cggcgacccg tccgaccagg
     1381 tgaacggcta ccatcccggc caggacgccg ccatggcgag gacgatcgaa cgactgctgg
     1441 ccgcccggtc ggtggtgacc gtgtccagtc gcacgaaggc actgggtgcg ctgcgctccc
     1501 tcggcgtcaa cccggacgca agctacgact ccgaaccgct gcgcacgctg caccgcacga
     1561 ccaaggacag cgactactac ttcctgttca actccagcac cgagcggacc agcaccacgg
     1621 tcacgttgaa aggcaggggc gtgccctacc gctacaacgc ctggaccggg gcggtcaccc
     1681 cgatcgctga gtactcgcgc acccgcagcg gcgtccgggt gcccgtcgac ctggccaccg
     1741 gccagggcga actgatcgcg ctgaccaggg gtaacgcgga cacgccgcga accaggtgca
     1801 ccccctcggc gctcagcacc accgcggacg aactcgtggt cgggtcgggc tccagtctgc
     1861 tgatgcggga cgcgaaggcc ggccggtact caaccaggct ggccgacgga cgccgggtga
     1921 tcagcaccat cgatacggtg gcggcacctg tcacgccgca gtcctggacg ctgaatgtga
     1981 cctcatggca cgccggcgcg agcccgtccg agaccgcgaa gacagccctg gacgagatca
     2041 tgctcacgac cggctccgac aaaacgttgc cgagttggca gaagatcgac ggcctggcga
     2101 actcctcggg caccgcggtc tactccacga agctcgacgt ggggccggcc tggaccggcg
     2161 ggacgggtgc ctacctcgat ctgggccagg tgctcggcac cgcgcaggtg tcggtgaacg
     2221 gaaggcaact gcccgcgctc gaccaggtcg gaaccggccg gatcgatctc ggcggttacc
     2281 tcaacgccgg cagcaacacc atcgaggtgc ggatcgccac gcccgtgaac aatgcggggt
     2341 tcggcaccaa ggatgcctac ggcctggtcg ggccggtgac gctcaccccg tacggtcagg
     2401 cctcgatcca ggtggcctgc cgacgctgac ctcgccaggc gcggaaatgc cccgctgtca
     2461 cggtccgcgg accgtgacag cggggcatcc gactcggtag gggttcagcc ggcggcaatg
     2521 ctcaggcgcc ccaggacgcc tgcgcaccac cgacgcgatc ggcctcgatc gtggccgcat
     2581 agccggacgc cgcgtaggcg gccatcgggt cggcgggcag gccgcgggac tcgcgccact
     2641 gcctcaggtc ggcgcgcacg tcggtataga aggcgtccat gtagacctgg ttggcaccga
     2701 gcacgtcgcc ggcctgctgg gcggccgaca gtgcgtctcg gtcgatcagc agggcgcggg
     2761 ccagcatctc ctgcacgttc agcaccgagc ggatctgccc gggaatcttc ggctcgatgt
     2821 tgtggcactg gtcgagcatg aagaccaggc cggacgccgg gtcgagaccg ccgccgcgga
     2881 ccacctcgta gagaatccgg aacagctgga acgggtccgc cgagccgacg atcaggtcgt
     2941 cgtcggcgta gaaccggctg ttgaagtcga acgaaccgag ccgtccgtgc cgcagcaact
     3001 gggtgacgat gaactcgatg ttggtgccgg gtgcgtggtg cccggtgtcg aggcagacgc
     3061 tcgccttctc gcccagcgcc aggcagtggg tcagcgaggt gccccagtcg ggcacgtcca
     3121 tgtggtagaa cgccggctcg aagaacttgt actccagcac cagccgctgc ttctcgccca
     3181 gcccgtcgta gatctcgcgc aatccctgcg ccagccggtc ctggcgggcg cgcaggtcgt
     3241 cctggcccgg gtagttggtg ccgtcggcca gccagatctt caggtcctgc acgccggtgg
     3301 cgtgcatgat ctcgatgcac tccaggtggt ggtcgatcgc cttgcggcga atccgggggt
     3361 cggggttgca cagcgagccg agccggtagt cgtcgtcctg gaacacgttg ctgttgatcg
     3421 tgccgatccg cacgccgcgg tcggcggcat gggcgccgag ggcgtcccaa tcgtccacct
     3481 tgtcccaggg aatgtgcagg gagaccgccg gcgccaggcc ggtcagggcg ttcacctggg
     3541 cggcgtcgtc gatcttctcg tacgggtcgc gcgggccgcc gggcagggtg aacaccttga
     3601 accgggtgcc cgagttgccg taggcccacg acggcacctc gatcgcgagg gtcgccagag
     3661 tgtcgagggt tgcttgatcg atggtcattg cggagtcctt tcgccggcgc tttctgcgcg
     3721 agtcgggcgc catcctccga cgcctccgtt agcctcggca tggatgaaac gtttcatcca
     3781 tcaatgtaga cgccgctgtt gggtagggtc aagacacgtg atccgtttca acccacagcg
     3841 cgcacacagg aggtcgtcat ggctgcgagc gtgaaggacg ttgcgcggca cgccggattg
     3901 tctgtgggta ccgtctcgaa cgtgctcaac cgtcccgaca gcgtcacgga ggccacgaag
     3961 gccaaggtga tggccgcgat cgacgaactg ggctacgtgc gcaacgaggc ggcccggcag
     4021 ttacgagccg gcatgagcca ctgcgtcggc gtgatcgtgc tcaactcgtc caacccgttc
     4081 ttcaacgatg tcgccgcggg cgccgaggcc tgggcggccg acgccaaggt gtcggtgctg
     4141 gtcggcaaca gcggcgaact ggtcgaacgc gagaaggcct acctcgacct gttcgaggaa
     4201 cagcgcgtcc gcggcgtact gctctccccc gtcgggcagg tcaccaagcg gctgcagcgc
     4261 atgcgcaagg gcggcatcgc gaccgtgctc gtcgaccgcg acatcgccgg cgacttcagc
     4321 tcggtgtcgg tggacgaccg cctgggcggc cggctggcca tggagcacct gatctcgacc
     4381 ggacgacggc gcatcgcctt cgttggcggg cccttgtcac ttggtcaggt cgcggaccgg
     4441 ttcgccggtg ccgccgaggt cgccggtgag cacgccgacg tgtttctcga gttcgtcgcc
     4501 accgaccggc tcaccctcgc ccacggacgc gtcgcgggcg agcggctgct cgcgcgcccc
     4561 gccggcaccc gtccggacgc ggtgttcgcc gccaacgacc tgctcgcgct cggactgctg
     4621 caggcgatcg ccagcacccg ccgcctgcgc gttccggacg acatcgccct gatcggctac
     4681 gacgacatcg acttcgccgc cggcgccgcc gtgccgctca gttcgatcgc ccagccgcga
     4741 ttcgagctcg gcaggctcgc catggacctg ctgctcaaag ccgcggacga cccttcacgt
     4801 ccggtcgaac gagtggtgct gcaaccgttc ctggtcgcac gcgcatccac ccagagcacg
     4861 tccagcacgc gctgatcgct gggcccgcac cctaccgcag gccgaatggg gctcttgtca
     4921 acccatgagc gttaggctat cgtccattgg aacgtttcaa aacgccaaag gaggcgctcg
     4981 tggacccatc ccagcagacg cccgtcctcg agctcaaagg tgtctccaag agcttcgggg
     5041 cggtggtggc actacgcaac gcggacctcg ctctgtacgc cggttccatt catgcattgg
     5101 tcggcgagaa tggcgccggc aaatccaccg tggtcaagat catggccggg ctgtatcagc
     5161 gcgatagcgg cacgttcgcc atggacggcg agcccgttca ttttcgcaac accgccgaag
     5221 ccaaggccgc aggaatagcg gtgatctatc aggagccgac cctgtttccg gatttgtcgg
     5281 taaccgagaa cgtgttcatg gggaaggagc tggcggcctc gcatcccaac atcaagctgg
     5341 tcgacaccgt ctacggcaac gacgacgacc aggtctcgtt cgacaagacc tccggcctgc
     5401 tgtcggcgca tccgaacctg aagggcatca tcagccccac caccgtcggc atcgcagcag
     5461 cggcccgcta cctgtcgaag tccgacgcca agggcaaggt cgcgctcacc ggcctcggca
     5521 cccccaacca gctgcgcgaa tacgtcaagg acggcacggt gaaggagttc gccctgtgga
     5581 acccgcagga tctcggcacc ctggccacct acgccgc
//
LOCUS       DAOSUG010000155         5515 bp    DNA     linear   ENV 10-JUN-2024
DEFINITION  MAG TPA_asm: Micropruina sp. isolate AMR_MDS_2195
            NODE_6674_length_5515_cov_3.825641, whole genome shotgun sequence.
ACCESSION   DAOSUG010000155 DAOSUG010000000
VERSION     DAOSUG010000155.1
DBLINK      BioProject: PRJNA925477
            BioSample: SAMN32864262
            Sequence Read Archive: SRR4236673
KEYWORDS    WGS; ENV; Metagenome Assembled Genome; MAG; Third Party Data; TPA;
            TPA:assembly.
SOURCE      Micropruina sp. (wastewater metagenome)
  ORGANISM  Micropruina sp.
            Bacteria; Bacillati; Actinomycetota; Actinomycetes;
            Propionibacteriales; Nocardioidaceae; Micropruina.
REFERENCE   1  (bases 1 to 5515)
  AUTHORS   Abdulkadir,N., Saraiva,J.P., Zhang,J., Stolte,S., Gillor,O.,
            Harms,H. and Rocha,U.
  TITLE     Genome-centric analyses of 165 metagenomes show that mobile genetic
            elements are crucial for the transmission of antimicrobial
            resistance genes to pathogens in activated sludge and wastewater
  JOURNAL   Microbiol Spectr 12 (3), e0291823 (2024)
   PUBMED   38289113
REFERENCE   2  (bases 1 to 5515)
  AUTHORS   Abdulkadir,N.
  TITLE     Direct Submission
  JOURNAL   Submitted (24-MAR-2023) Environmental Microbiology, Helmholtz
            Center for Environmental Research -UFZ, Permoserstr 15, Leipzig
            04318, Germany
COMMENT     The annotation was added by the NCBI Prokaryotic Genome Annotation
            Pipeline (PGAP). Information about PGAP can be found here:
            https://www.ncbi.nlm.nih.gov/genome/annotation_prok/
            
            ##Genome-Assembly-Data-START##
            Assembly Method        :: SPAdes v. 3.11.1
            Genome Representation  :: Full
            Expected Final Version :: Yes
            Genome Coverage        :: Not Applicable
            Sequencing Technology  :: Illumina MiSeq
            ##Genome-Assembly-Data-END##
            
            ##Genome-Annotation-Data-START##
            Annotation Provider               :: NCBI
            Annotation Date                   :: 05/04/2023 04:57:10
            Annotation Pipeline               :: NCBI Prokaryotic Genome
                                                 Annotation Pipeline (PGAP)
            Annotation Method                 :: Best-placed reference protein
                                                 set; GeneMarkS-2+
            Annotation Software revision      :: 6.5
            Features Annotated                :: Gene; CDS; rRNA; tRNA; ncRNA
            Genes (total)                     :: 2,776
            CDSs (total)                      :: 2,750
            Genes (coding)                    :: 2,714
            CDSs (with protein)               :: 2,714
            Genes (RNA)                       :: 26
            tRNAs                             :: 24
            ncRNAs                            :: 2
            Pseudo Genes (total)              :: 36
            CDSs (without protein)            :: 36
            Pseudo Genes (ambiguous residues) :: 0 of 36
            Pseudo Genes (frameshifted)       :: 2 of 36
            Pseudo Genes (incomplete)         :: 32 of 36
            Pseudo Genes (internal stop)      :: 2 of 36
            ##Genome-Annotation-Data-END##
FEATURES             Location/Qualifiers
     source          1..5515
                     /organism="Micropruina sp."
                     /mol_type="genomic DNA"
                     /submitter_seqid="NODE_6674_length_5515_cov_3.825641"
                     /isolate="AMR_MDS_2195"
                     /isolation_source="Activated sludge of municipal
                     wastewater treatment plants"
                     /db_xref="taxon:2737536"
                     /environmental_sample
                     /geo_loc_name="Canada"
                     /lat_lon="49.25 N 123.1 W"
                     /collection_date="2015-06-16"
                     /metagenome_source="wastewater metagenome"
                     /note="metagenomic"
     gene            582..654
                     /locus_tag="PLF56_06395"
     tRNA            582..654
                     /locus_tag="PLF56_06395"
                     /product="tRNA-Lys"
                     /inference="COORDINATES: profile:tRNAscan-SE:2.0.12"
                     /note="Derived by automated computational analysis using
                     gene prediction method: tRNAscan-SE."
                     /anticodon=(pos:615..617,aa:Lys,seq:ctt)
     gene            complement(966..1880)
                     /locus_tag="PLF56_06400"
     CDS             complement(966..1880)
                     /locus_tag="PLF56_06400"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_012918928.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_process: GO:0008643 - carbohydrate transport [Evidence
                     IEA];
                     GO_process: GO:0055085 - transmembrane transport [Evidence
                     IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="carbohydrate ABC transporter permease"
                     /protein_id="HOQ53229.1"
                     /translation="MTTAQAQVSQAELAQLNKPRRDGRYWAGAIGGIAGIAFIIIYCV
                     LPFYWMIVSSLRQPADGRSTDFLPNPASIENYVAVFAPQNMFGTALINSLIVSTTTTV
                     LTLLFGILAAYALARLRFAAKGIVLSIIIACSMFPGITLVIPLFKLFSGNYPGFEWMN
                     WMNTYQALIIPTLSFGLPLCVWNLTAFFRQLPVELEQAAMVDGCTPGQAFAKVILPLA
                     APGVFTTAIITFIAVWNDYMIALQFGTRPEMYTSIVAVSKFTGPIPYVTPWGAIMAAG
                     VVITLPLLIGVLIFQRRIVAGLTAGGVK"
     gene            complement(1877..2944)
                     /locus_tag="PLF56_06405"
     CDS             complement(1877..2944)
                     /locus_tag="PLF56_06405"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_020392413.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_function: GO:0042626 - ATPase-coupled transmembrane
                     transporter activity [Evidence IEA];
                     GO_function: GO:0140359 - ABC-type transporter activity
                     [Evidence IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="sugar ABC transporter permease"
                     /protein_id="HOQ53230.1"
                     /translation="MVADSPATTNKKKNPLSDGQGTLAAILISPTMAVILFVVGIPIL
                     MSLRESVFRKFDTVDPSTGMIPEGETFVGLQNFSDIFNNPTIVPGNWGQIDRLLNAFA
                     NTTWFTIVCVLLETVLGVAMALIMAKAFKGQGFVRAAILVPWAIPTVVSAKMWGLIFD
                     ADGVMNEVIGHQVLWLADGPQSQWAIIIADVWKTAPFIGLLTLAGLQTIPAEVYEAAK
                     VDGANAWQQFTRITLPMVKPALVVAVLFRTLDTMRMFDLPAAMIGFGKYYVETVSTLA
                     YVENNNLRYGAAAAYSIILFLYIVFVAFLFVRLLGADVVGDEEVKAIAAQRKRQRAAA
                     KAAGRKPELAKTTVTSGSASS"
     gene            complement(3020..4309)
                     /locus_tag="PLF56_06410"
     CDS             complement(3020..4309)
                     /locus_tag="PLF56_06410"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_012918926.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="ABC transporter substrate-binding protein"
                     /protein_id="HOQ53231.1"
                     /translation="MIAAALSVGALLVTAACSSGTAASPAASGGASSAAATGADWNEF
                     GPIVYAQGKDTSGNLKDKVDEWNKANPNEQVTFRELSDSADEQRAAMIQRGQAKSGEY
                     TVMSVDVVWTAEFAANGWLTELPADKFPTTGYLKPAVDSATYFNKLYAMPSTSDGALL
                     YYRKDLLDKAGLQPPKTWDEMQAACDKVLPGQSGMSCYGGQFQKYEGLTCNFAEAVNS
                     AGGAVLGEDGKPTVNSADANAGLTWMVNGFKTGMIPKEAITWKEEESRTAFQNSKILF
                     LRNWPYVWNLANAKDSKIAGKFAVAPIPGKNGPGVSTLGGHSYGISPFAKNKGTAMKW
                     INWMLTEENQKKWLQTASSAPVLESLYADAELNKQFPYLATLGESIKNAKPRPKAVKY
                     GDVTLAIQDAAYGAIQNQSAPQAALDGLQTKLQALVK"
     gene            complement(4549..4854)
                     /locus_tag="PLF56_06415"
     CDS             complement(4549..4854)
                     /locus_tag="PLF56_06415"
                     /inference="COORDINATES: protein motif:HMM:NF023697.2"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="DUF3618 domain-containing protein"
                     /protein_id="HOQ53232.1"
                     /translation="MADTTTRSKQDIEAEIAAARARLADNVAGLINQVHPKAVVQRGI
                     DDARGFAAAEFANVRSQVMDDRGQVRTERVMLIGGAIAGVVTFLVLVRKIIRGTRRG"
     gene            4943..5431
                     /locus_tag="PLF56_06420"
     CDS             4943..5431
                     /locus_tag="PLF56_06420"
                     /EC_number="1.11.1.24"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_013160694.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_function: GO:0008379 - thioredoxin peroxidase activity
                     [Evidence IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="peroxiredoxin"
                     /protein_id="HOQ53233.1"
                     /translation="MTKLAQGDVAPAFTLPDSDARPVSLSDYARRRVIVYFFPAAMTP
                     GCTIQAVDFSSALTQIAAAGYDVVGISPDSVEKLAKFRSQEDLGFPLLADPDKSVLTA
                     YGAFGRKMLYGKEIEGVIRSTFVVSVDDAGTGRVDVAQYNVKATGHVAKLRRELKIPD
                     LG"
BASE COUNT          954 a         1795 c         1774 g          992 t
ORIGIN      
        1 atcgccagcg ggtcgcgttc gtgcgcaagc agggccttcg tcgctgactc acccccgcca
       61 cccgtgatcc cgacggacgc ctagccatcc gggcgaacgc ctcgcatccc ggcgaacgcc
      121 tcgccgtccc ggcggacccc gcacatcatc ggacgaggtc gccgagccgc gctttgcgac
      181 ggtgggacgc caaccaggct ctccaactgc tccagtggcc tacgccgccg ccgttgacga
      241 actgcccgcg tacctgcccg tcatcccggc gaacgccggg atctccccac gcacccacgg
      301 ccgcccgttc gcccgcactc acgaccatga ccgcccggga gctgtccgaa ccgtttgtgg
      361 tacgcggctc cagtacggcg tgcacgacgt cgaggtgcag tcctgggtgc gggccgaccg
      421 cgccggcttg gacgcccgct cgccgatgcc gtggacggct ggatcgagaa ggcgtcggcc
      481 ctggcgacga ccgcggcgct tggggtgttg agccggagga tgcaggaatc accgtggtgg
      541 atttcacctt gcggcaaggc ttcggtatag tttcttaccg cgcgccgcta gctcaactgg
      601 cagagcagct ggctcttaac tagcgggttc ggggttcgag tccctggcgg cgcacaacgg
      661 aaggccctgc gaaagcaggg ccttttcgct tgcccggtcg agaacacccc gtcggtcgag
      721 cagtgttcag gtcctctcgc gcgacgcctc cctgccggct gagatcccgg cgttcgccgg
      781 gatgacgggg ggtcgccggg acgacggggt cgccgggacg acgctggtcg ccgggacgac
      841 gctggtcgcc gggacgacgc tggtcggtgg gatcccggcg tccgggatgg cgagattccg
      901 acgggctggc atgcaacagg ggcgcccggt gaaccggacg cccctgcgct ctcaaagggt
      961 gcgcgttact tcacgccacc ggcggtcagg ccggcgacga tgcggcgctg gaagatcagc
     1021 acgccgatca gcagcggcag ggtgatcacc acaccggccg ccatgatggc gccccacggc
     1081 gtcacgtagg ggatcgggcc ggtgaacttc gacaccgcga cgatcgaggt gtacatctcc
     1141 ggacgggtac cgaactgcag cgcgatcatg tagtcgttcc acaccgcgat gaaggtgatg
     1201 atcgccgtgg tgaacacgcc cggcgcggca agcggaagga tcaccttcgc gaacgcctgg
     1261 cccggcgtgc agccgtccac catggcagcc tgctcgagct cgaccggcaa ctgccggaag
     1321 aacgcggtca ggttccacac gcacagcggc agaccgaacg acagggtggg gatgatcagc
     1381 gcctggtagg tgttcatcca gttcatccac tcgaaacccg ggtagttgcc gctgaacagc
     1441 ttgaacagcg gaatcaccaa cgtgatgccg ggaaacatcg agcaggcgat gatgatgctc
     1501 agcacgatgc ccttggcggc gaaccggagc ctggccagcg cgtacgccgc cagaatgccg
     1561 aacagcagcg tgagcaccgt ggtggtcgtc gagacgatca gcgagttgat cagagcggtg
     1621 ccgaacatgt tctgcggagc gaagaccgcg acatagttct cgatcgacgc cgggttgggc
     1681 agaaagtccg tgctgcggcc gtccgcgggc tgccgcagcg aggacacgat catccagtag
     1741 aacggcagca cgcagtagat gatgatgaac gcgatgccgg cgatgccgcc gatcgcaccg
     1801 gcccagtagc ggccatcgcg ccggggcttg ttcagttggg ccagttcagc ctggctcacc
     1861 tgtgcctgtg ctgtggtcat gatgatgcac tcccggaggt gacggtggtc ttggccagtt
     1921 cgggcttcct gccggcggcc ttggccgccg ccctctgccg cttgcgctgt gccgcgatcg
     1981 ccttgacctc ttcgtcaccg acgacgtcgg caccaagcag gcggacgaac aggaacgcca
     2041 cgaacacgat gtagaggaac aggatgatcg agtacgccgc agccgcgccg tagcgcaggt
     2101 tgttgttctc gacgtacgcc agtgtcgaca cggtttcgac gtagtacttg ccgaaaccga
     2161 tcatggcggc tggcaggtcg aacatgcgca tcgtgtcgag cgttcggaac agcaccgcca
     2221 ccaccagtgc gggtttgacc atcggcaacg tgatgcgagt gaactgttgc caggcgtttg
     2281 ctccatcgac cttggcggcc tcgtacacct cggccggaat ggtctgcagg ccggccagtg
     2341 tgagcaggcc gatgaacggc gctgtcttcc agacgtcggc gatgatgatc gcccactgag
     2401 actgcggacc gtccgcgagc cacaggacct gatgcccgat gacctcattc atcacaccat
     2461 cggcgtcgaa gatcagcccc cacatcttgg ctgagacgac ggtcggtatc gcccaaggca
     2521 ccagaatggc ggccctaacg aatccttgtc ccttgaacgc cttcgccatg atcagggcca
     2581 tcgcgacgcc caacaccgtc tcgagcagca cgcagacgat cgtgaaccac gtggtgttcg
     2641 caaaggcatt caacaggcgg tcgatctggc cccagtttcc gggaacaatc gtgggattgt
     2701 tgaagatatc actgaagttc tgcagtccga caaacgtctc cccctcggga atcatgcccg
     2761 tgctcggatc aaccgtgtcg aacttgcgga ataccgattc gcgtagcgac atcagaatcg
     2821 ggatgcccac cacgaacagg atgaccgcca tggtcggcga gatcagaatg gcggctaagg
     2881 tgccctgacc gtcgctgagt ggattcttct ttttgtttgt ggttgctggt gaatcagcca
     2941 ccatcgcacc tcctccaggt tgacggctcg aggggtgccg ggcgcgatgc ccggcacccc
     3001 cctaacctga atcaccaggt cacttgacca atgcctgcag cttggtctgc aatccgtcga
     3061 gtgcagcctg cggcgcgctc tggttctgaa tggcgccgta ggcggcgtcc tgaatggcca
     3121 gagtcacgtc accgtacttg acggccttcg ggcgcggctt ggcgttcttg atcgactcac
     3181 cgagcgtggc caggtacggg aactgcttgt tcagttccgc gtcggcgtac agcgactcga
     3241 gcaccggagc gctggaggcc gtctgcagcc acttcttctg gttctcttcg gtgagcatcc
     3301 agttgatcca cttcatcgcg gtgcccttgt tcttggcgaa gggcgagatg ccgtagctgt
     3361 ggccaccgag ggtcgagacg cccggaccgt tcttgccggg gatcggcgcg accgcgaatt
     3421 tgcccgcgat cttgctgtcc ttggcgttgg ccaggttcca cacgtagggc cagttgcgca
     3481 ggaacaggat cttgctgttc tggaacgcgg tgcgcgactc ctcttccttc caggtgatgg
     3541 cttccttcgg gatcatgccc gtcttgaaac cgttcaccat ccaggtcagg ccggcgttgg
     3601 cgtcggccga gttcaccgtg ggcttaccgt cctcaccgag gacggcgccg ccggcggagt
     3661 tgaccgcctc ggcgaagttg caggtcaggc cctcgtactt ctggaactgg ccgccgtagc
     3721 acgacatgcc ggactggccg ggcagcacct tgtcacaggc cgcctgcatc tcgtcccagg
     3781 tcttgggcgg ctgcaggccc gccttgtcga gcaggtcctt gcggtagtac agcagcgcgc
     3841 cgtccgaggt ggagggcatc gcgtacagct tgttgaagta ggtggcggaa tcgacggccg
     3901 gcttgaggta gccggtggtc gggaacttgt cggcgggcag ttcggtcagc cagccgttgg
     3961 cggcaaactc ggcggtccac acgacgtcga cgctcatgac ggtgtactcg cccgacttgg
     4021 cctggccgcg ctggatcatg gcggcgcgtt gctcgtcggc ggaatcggac agttcacgga
     4081 aggtgacctg ctcgttgggg ttggccttgt tccactcgtc gaccttgtct ttcaggttgc
     4141 ccgaggtgtc tttgccctgg gcgtagacga tcgggccgaa ctcgttccag tcggcgccgg
     4201 tggcggccgc gctgctggca ccgccggacg cggccggcga ggctgcagta cccgaggagc
     4261 acgccgcggt caccagcagg gctccgaccg acaaagcagc agcgatcacc cgccgtgtgg
     4321 tgcgtggttg agtcatcaat cctcacttca ttgggaaacg gccccactac cgagaccagg
     4381 gcccctcctg tgggacctgc gagggactgt atccatacca atgcgccagc gctagcgact
     4441 cgccagatcg ttatcaaccg ttgaccttta gagacaccga tagccgctgc gacaaggcaa
     4501 agcaatgcaa caccgatgcc cccaccggtg ttgcgtgatg gtcacagctc agccccgccg
     4561 agttcccctg atgattttgc ggaccaaaac caggaaggtg acgacccctg caatggcgcc
     4621 gccgatgagc atgacccgtt cagtacgcac ctgcccacga tcgtccatca cttgggatct
     4681 tacgttcgcg aactcggcgg ctgcgaaccc gcgggcgtcg tcgatgccgc gctggaccac
     4741 cgccttggga tggacctgat tgatcaggcc ggccacgttg tcggccagcc tggcccgggc
     4801 agcggcgatc tcggcctcga tgtcctgctt gctgcgagtg gtggtgtcgg ccacaggagt
     4861 tccttcgctt cggtgaaagt ccaccctatg ccagctttcg ggcccgcggg caccccccag
     4921 cgtgtgttct agagtgatgg ccatgacgaa gctcgcccag ggagacgttg cccctgcctt
     4981 caccctgccg gactccgacg cacgcccggt ctcgctctcc gactacgccc gacgccgggt
     5041 gatcgtctac ttctttccgg ccgcgatgac gcccggctgc accatccagg cggtcgactt
     5101 ctccagcgcc cttacccaga tcgcggccgc cggctacgac gtggtgggca tctcgcccga
     5161 cagcgtcgag aagttggcca agtttcgaag ccaggaagac ctgggctttc cgctactcgc
     5221 cgaccccgac aagtcggtcc tcaccgccta cggtgccttc ggccgcaaga tgctctacgg
     5281 caaagagatc gagggcgtga ttcgttccac gttcgtggtc agcgtggacg acgccggcac
     5341 cggccgggtg gacgtcgccc aatacaacgt caaagccacc ggccatgtgg caaagctgcg
     5401 tcgcgaactg aagattcccg atctgggctg acgactccgc ccgaatcatg gtcgagcgtg
     5461 tcgagaccgc gtgagcagca gcggcgacac catcgagccg tctggtcaga ctgac
//
LOCUS       DAOSUG010000156         5508 bp    DNA     linear   ENV 10-JUN-2024
DEFINITION  MAG TPA_asm: Micropruina sp. isolate AMR_MDS_2195
            NODE_6683_length_5508_cov_3.804328, whole genome shotgun sequence.
ACCESSION   DAOSUG010000156 DAOSUG010000000
VERSION     DAOSUG010000156.1
DBLINK      BioProject: PRJNA925477
            BioSample: SAMN32864262
            Sequence Read Archive: SRR4236673
KEYWORDS    WGS; ENV; Metagenome Assembled Genome; MAG; Third Party Data; TPA;
            TPA:assembly.
SOURCE      Micropruina sp. (wastewater metagenome)
  ORGANISM  Micropruina sp.
            Bacteria; Bacillati; Actinomycetota; Actinomycetes;
            Propionibacteriales; Nocardioidaceae; Micropruina.
REFERENCE   1  (bases 1 to 5508)
  AUTHORS   Abdulkadir,N., Saraiva,J.P., Zhang,J., Stolte,S., Gillor,O.,
            Harms,H. and Rocha,U.
  TITLE     Genome-centric analyses of 165 metagenomes show that mobile genetic
            elements are crucial for the transmission of antimicrobial
            resistance genes to pathogens in activated sludge and wastewater
  JOURNAL   Microbiol Spectr 12 (3), e0291823 (2024)
   PUBMED   38289113
REFERENCE   2  (bases 1 to 5508)
  AUTHORS   Abdulkadir,N.
  TITLE     Direct Submission
  JOURNAL   Submitted (24-MAR-2023) Environmental Microbiology, Helmholtz
            Center for Environmental Research -UFZ, Permoserstr 15, Leipzig
            04318, Germany
COMMENT     The annotation was added by the NCBI Prokaryotic Genome Annotation
            Pipeline (PGAP). Information about PGAP can be found here:
            https://www.ncbi.nlm.nih.gov/genome/annotation_prok/
            
            ##Genome-Assembly-Data-START##
            Assembly Method        :: SPAdes v. 3.11.1
            Genome Representation  :: Full
            Expected Final Version :: Yes
            Genome Coverage        :: Not Applicable
            Sequencing Technology  :: Illumina MiSeq
            ##Genome-Assembly-Data-END##
            
            ##Genome-Annotation-Data-START##
            Annotation Provider               :: NCBI
            Annotation Date                   :: 05/04/2023 04:57:10
            Annotation Pipeline               :: NCBI Prokaryotic Genome
                                                 Annotation Pipeline (PGAP)
            Annotation Method                 :: Best-placed reference protein
                                                 set; GeneMarkS-2+
            Annotation Software revision      :: 6.5
            Features Annotated                :: Gene; CDS; rRNA; tRNA; ncRNA
            Genes (total)                     :: 2,776
            CDSs (total)                      :: 2,750
            Genes (coding)                    :: 2,714
            CDSs (with protein)               :: 2,714
            Genes (RNA)                       :: 26
            tRNAs                             :: 24
            ncRNAs                            :: 2
            Pseudo Genes (total)              :: 36
            CDSs (without protein)            :: 36
            Pseudo Genes (ambiguous residues) :: 0 of 36
            Pseudo Genes (frameshifted)       :: 2 of 36
            Pseudo Genes (incomplete)         :: 32 of 36
            Pseudo Genes (internal stop)      :: 2 of 36
            ##Genome-Annotation-Data-END##
FEATURES             Location/Qualifiers
     source          1..5508
                     /organism="Micropruina sp."
                     /mol_type="genomic DNA"
                     /submitter_seqid="NODE_6683_length_5508_cov_3.804328"
                     /isolate="AMR_MDS_2195"
                     /isolation_source="Activated sludge of municipal
                     wastewater treatment plants"
                     /db_xref="taxon:2737536"
                     /environmental_sample
                     /geo_loc_name="Canada"
                     /lat_lon="49.25 N 123.1 W"
                     /collection_date="2015-06-16"
                     /metagenome_source="wastewater metagenome"
                     /note="metagenomic"
     gene            <1..561
                     /locus_tag="PLF56_06425"
     CDS             <1..561
                     /locus_tag="PLF56_06425"
                     /inference="COORDINATES: protein motif:HMM:NF012503.2"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_function: GO:0016831 - carboxy-lyase activity [Evidence
                     IEA];
                     GO_function: GO:0030170 - pyridoxal phosphate binding
                     [Evidence IEA];
                     GO_process: GO:0019752 - carboxylic acid metabolic process
                     [Evidence IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="pyridoxal-dependent decarboxylase"
                     /protein_id="HOQ53234.1"
                     /translation="DGLELVDSYSTNPHKWMGITFDCNLFYTADRASLLGALSILPEY
                     LRTAAAETGAVIDYRDWQVPLGRRFRALKLWFTLRTDGVAPAQAMIRDHVAWTQELAG
                     WVRADDRFEIVAPHPLNLLCLALRAGDGPTAGLIERANATGSVLFTRTVLDARTVLRF
                     SIGARRTRHQDVRAAWNLLRDLAGGQ"
     gene            complement(708..1079)
                     /locus_tag="PLF56_06430"
     CDS             complement(708..1079)
                     /locus_tag="PLF56_06430"
                     /EC_number="5.2.1.8"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_017935421.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_function: GO:0003755 - peptidyl-prolyl cis-trans
                     isomerase activity [Evidence IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="FKBP-type peptidyl-prolyl cis-trans isomerase"
                     /protein_id="HOQ53235.1"
                     /translation="MTLEKPEVDFPGDAPDDLQISDIVVGTGEEATAGSTVIVHYVGV
                     AHSSGEEFDASYNRGEPLRFKVGVGQVISGWDKGIVGMKVGGRRQLVIPPHLGYGDRG
                     AGGVIAPGETLIFVVDLLGVR"
     gene            1163..2452
                     /gene="hisD"
                     /locus_tag="PLF56_06435"
                     /pseudo
     CDS             1163..2452
                     /gene="hisD"
                     /locus_tag="PLF56_06435"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_002520526.1"
                     /note="incomplete; partial in the middle of a contig;
                     missing C-terminus; Derived by automated computational
                     analysis using gene prediction method: Protein Homology.
                     GO_function: GO:0004399 - histidinol dehydrogenase
                     activity [Evidence IEA];
                     GO_process: GO:0000105 - histidine biosynthetic process
                     [Evidence IEA]"
                     /pseudo
                     /codon_start=1
                     /transl_table=11
                     /product="histidinol dehydrogenase"
     gene            2815..3909
                     /locus_tag="PLF56_06440"
     CDS             2815..3909
                     /locus_tag="PLF56_06440"
                     /EC_number="2.6.1.9"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_002548972.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_function: GO:0004400 - histidinol-phosphate
                     transaminase activity [Evidence IEA];
                     GO_function: GO:0030170 - pyridoxal phosphate binding
                     [Evidence IEA];
                     GO_process: GO:0000105 - histidine biosynthetic process
                     [Evidence IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="histidinol-phosphate transaminase"
                     /protein_id="HOQ53236.1"
                     /translation="MKPEPTLADLPLRPELVGEEPYGAPQLDVPICLNVNENPFAPSP
                     EIAADMARALTAGATGLNRYPDREATGLRTALAAYLGHELTLDNVWAANGSNEVMTHV
                     LGAFGGPGRTLLAFTPTYSMYPEYARNTHTRYVTAPRHDDFTLTADLVLRAIADTEAD
                     VVIITTPNNPTGTPTPLAVTEAVLENTDAIVVVDEAYQEFSRHPEQTSLTLLPRFGNL
                     IVSRTMSKAFALAGGRLGYLAAAPAIVNACRIVRLPYHLSSQTQLVAETALKHAGFLL
                     GQVDQLRRTRDEMLVRLPRLGVEVIDSDANFCLFGPFADRHDVWQRLLDRGVLVRETG
                     PAGWLRVSVGTPHETELFYQALAEVLEEIR"
     gene            3906..4520
                     /gene="hisB"
                     /locus_tag="PLF56_06445"
     CDS             3906..4520
                     /gene="hisB"
                     /locus_tag="PLF56_06445"
                     /EC_number="4.2.1.19"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_002513559.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_function: GO:0004424 - imidazoleglycerol-phosphate
                     dehydratase activity [Evidence IEA];
                     GO_process: GO:0000105 - histidine biosynthetic process
                     [Evidence IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="imidazoleglycerol-phosphate dehydratase HisB"
                     /protein_id="HOQ53237.1"
                     /translation="MKARTASVERSTSESIVKVSVNLDGSGLATIDTGVGFYDHMLTA
                     LAKHSLIDLDVRTIGDLQIDGHHSIEDTAIVLGQAVREALGDKRGIVRFGDATVPLDE
                     ALAHAVVDVAGRPYAVCSGEPEAQIYALIGGSGVPYAGSMTYHLMESFAINAGICLHL
                     RLLAGRDPHHIVEAQFKAVARALRIAFAIDPRVADAIPSTKGAL"
     gene            4517..5140
                     /gene="hisH"
                     /locus_tag="PLF56_06450"
     CDS             4517..5140
                     /gene="hisH"
                     /locus_tag="PLF56_06450"
                     /EC_number="4.3.2.10"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_013863736.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_function: GO:0000107 - imidazoleglycerol-phosphate
                     synthase activity [Evidence IEA];
                     GO_function: GO:0016884 - carbon-nitrogen ligase activity,
                     with glutamine as amido-N-donor [Evidence IEA];
                     GO_process: GO:0000105 - histidine biosynthetic process
                     [Evidence IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="imidazole glycerol phosphate synthase subunit
                     HisH"
                     /protein_id="HOQ53238.1"
                     /translation="MTAPRVVVLDYGSGNLHSATRALAAAGADVTLTADSRAASAADG
                     LVVPGVGAFASCMDGLRAVGGPQLITDRLARGRPVLGICVGHQVMFGHGVEHGHDAAG
                     VGVLPGVVERLNSDRLPHMGWNTIEPPPGSTLFAGLAGERFYFVHSYAVRTADRLADD
                     AHVTWAEHGGDRFVAAVEVGGLSSTQFHPEKSGRAGAALLSNWLATL"
BASE COUNT          820 a         1962 c         1884 g          842 t
ORIGIN      
        1 gacggcttgg agttggtcga cagctacagc accaacccgc acaagtggat gggcatcacc
       61 ttcgactgca acctgttcta caccgccgac cgggccagcc tgctgggtgc tctcagcatt
      121 ctgccggagt acctgcgcac cgccgccgcc gagaccggtg ccgtgatcga ctaccgcgac
      181 tggcaggtgc cgctgggacg ccgattccgg gcgctcaagt tgtggttcac cctgcgcacg
      241 gacggggtgg cgccggccca ggcgatgatc cgggaccacg tcgcatggac gcaggagttg
      301 gccggctggg tgcgggcgga cgaccggttc gagatcgtgg cgccgcaccc gttgaacctg
      361 ctctgcctgg cgctccgcgc gggtgacggg ccgaccgccg gactgatcga gcgtgccaac
      421 gcgaccggtt cggtgctgtt cacgcgcacg gtgctcgacg ctcgcacggt gcttcgcttc
      481 tcgatcggcg cccgccgcac ccgccaccag gacgtccgcg cagcctggaa cctgctgcgc
      541 gacctggctg ggggtcagta actccgccgg ccgagcggtg tctcgacaag ctcgaccagc
      601 gggtctcgcc aagctcgacc aacgggcccc gacaagctcg accaacgggc cccgacgatc
      661 gctgaccagt gggcctcgac cagccggccc ccgccagcga acgtccctca gcgcacgccc
      721 agcagatcga cgacgaagat cagcgtctcc ccgggcgcga tcacgccgcc cgcgccacga
      781 tcgccatagc ccaggtgggg cggaatgacg agttggcgcc ggccgccgac cttcatgccg
      841 acgattccct tgtcccagcc gctgatcacc tgacccacgc ccaccttgaa cctgagcggc
      901 tcaccgcggt tgtagctggc gtcgaactct tcaccgctgg aatgcgcgac gccgacgtaa
      961 tgcacgatca cggtgctgcc tgccgtggcc tcttcgccgg tgcctacgac gatgtcgctg
     1021 atctgcaggt cgtcgggggc gtcgccggga aaatcgactt cgggcttctc aagagtcatg
     1081 cggcaagcct tacacaggga ggacgcgctt gccagacggg cagggcggtc gcgagccggg
     1141 cggcgtccgt agaatcggga gggtgatccg cacccttgac ctgcgtgggc aggccgtcga
     1201 ctaccgcacc gtcgttccgc gcgccccgat ggacgtcgaa cacgcggtcg agcgggtgcg
     1261 tccgatctgt gacgcggtcg ccagccgtgg ggtggacgcc ctgcgcgagt tctccgagcg
     1321 cttcgatcgg tgtgtgccca cgtcgttcct ggtgcccgcg gacgtcatcg ctgctgccga
     1381 gcgcgacctg accccgcagt tgcgcgccgc cctggtcgag tcgatccgcc gtcggcgagc
     1441 ggtcgccgaa ctcgaagcgc gggcagaaac caggcgcgcc gaacccgcgc cgggagcggt
     1501 ggtcgaggct cgtacgacgc ccgtcgaccg ggtcggcctg tacgtgcccg ggggactggc
     1561 gccgctggcg tcctcggtgc tgatgaacgt ggtgcccgct caggtggcgg gcgtgccgtc
     1621 catcgccgtt gcgtcgcccc cgcaggccga gttcggcggg cagccgcacc cgacgattct
     1681 ggccgtgtgc gcgttgctcg gcgtgaccga ggtgtatgcg gtcggcggag cacaggcgat
     1741 cgcgatgttc gcccacggcg tgccagggct gtgcgagccg gtcaacctgg tcaccggccc
     1801 cggcaacatc tacgtggtgg ccgccaaacg attgctgcgc ggactggtcg gcatcgactc
     1861 cgaggccggc ccgaccgaga tcgcgatcct ggccgacgac accgccgatg cccgctacgt
     1921 cgccgccgac ctgatcagcc aggccgagca cgatccgctg gccggggcgg tgctggtcac
     1981 cgattcgatc gagttggcgc aggccgtccg caccgaactc gaaccgcagg tggcggccac
     2041 ccggcacgcc gagcgcgtcc ggacggcgct ggcggggcaa caatccgcga tcgtgctggt
     2101 gaacgatctc gaccagggca tcgacgtggt caacgcctat gccgccgaac acctcgagat
     2161 tcagacccgc gacgcccgcg cagtcgccga tcgcatccgc aacgccggcg ccatcttcgt
     2221 cggcccgtgg tcgccggtct cgctcggtga ctattcggca ggcagtaccc acgtgttacc
     2281 gaccgccggg tgtgcctgcc acagttccgg gctgaacgtg aacagctttc tcaagcaggt
     2341 gcacgtgatc gactactcac gctcggcgct gatggcggtg gcgaacgggg tcgagacctt
     2401 tgctgccgcc gagaacctgc ctgcccacgg tgcggcggtc acggtgcggc gggacgctgg
     2461 agcggtcacg ggtgggtcgg gcgacgagat cccggcgtcc gccgggatga cggatgcctc
     2521 cgctggtccg ggtgggtcgg gtgacgagat cccggcgtcc gccgggatga cggatgcctc
     2581 cgctggtccg ggtgggtcgg gcgacgagat cccggcgtcc gccgggatga cggattcctc
     2641 cggtggtccg ggtgggttgg gtgacaagat cccggcgtcc gccgggatga cggattcctc
     2701 cggtggtccg ggtgggtcgg gtgaggagat cccggcgtcc gccgggatga cggatgcctc
     2761 ccgtcatccc gggcttgccc cgggatctgg ggccgcacgg tccgaggtcg tccggtgaaa
     2821 cccgaaccga ccctggccga cctgccgctg cgtcctgagt tggtcggcga agagccctac
     2881 ggcgcaccgc aactcgacgt gccgatctgc ctcaacgtca acgagaaccc ctttgcgccg
     2941 agcccagaga tcgccgccga catggcccgc gctctcacgg cgggcgcgac cgggctcaac
     3001 cggtaccccg accgcgaggc caccgggctg cgcaccgctc tggccgccta ccttggccat
     3061 gagttgaccc tcgacaacgt gtgggcggcg aacggctcga acgaggtgat gacccacgtg
     3121 ctcggcgcct tcggcggccc cggacgcacc ctgctcgcct tcacccccac ctattccatg
     3181 taccccgaat acgctcgcaa cacccacacc cgctacgtca ccgcgccgcg gcacgacgac
     3241 ttcacgctga ccgccgacct cgtgctgcgc gcgatcgccg acaccgaggc cgacgtggtg
     3301 atcatcacca cacccaacaa cccgaccggc acgcccaccc cgctcgccgt gaccgaggcc
     3361 gtgctcgaga acaccgacgc catcgtggtg gtcgacgagg cctaccagga gttcagccga
     3421 caccccgaac agacgtcgct gaccctgctg ccccgcttcg gcaacctgat cgtcagccgc
     3481 accatgagca aggccttcgc cctggccggc ggacgactcg gctacctcgc cgccgcaccc
     3541 gccatcgtga acgcctgccg catcgtgcgg ctgccctacc acctgtcgtc ccaaactcaa
     3601 ctggtcgccg agacggcgct gaagcacgcc ggatttctgc tcggccaggt cgatcagctg
     3661 cggcgcaccc gcgacgagat gctggtccgg cttcctcgac tcggggtcga ggtgatcgac
     3721 tccgatgcca acttctgttt gttcggtccc ttcgccgacc ggcatgatgt gtggcagcgc
     3781 ctgctcgatc ggggtgtgct cgtccgcgag accggaccgg cgggttggct acgggtttcg
     3841 gtcggcaccc cgcacgagac cgagttgttc taccaggcac ttgcagaggt tttggaggag
     3901 attcgatgaa ggcacgcacc gcgagcgtcg agcggtcgac cagcgagtcg atcgtgaagg
     3961 tgtcggtgaa cctggacggc agcggcctgg ccaccatcga caccggcgtc gggttctacg
     4021 accacatgct gaccgccctg gccaagcact ccctgatcga cctcgacgtg cgcaccatcg
     4081 gcgacctgca gatcgacgga catcactcca tcgaagacac cgccatcgtg ctgggccaag
     4141 ccgtccgcga ggcactgggc gacaagcgcg gcatcgtccg cttcggcgac gccaccgtgc
     4201 cgctcgacga ggccctggcg cacgccgtgg tggacgtcgc gggccgaccg tacgcggtct
     4261 gctccggcga accggaggcc cagatctacg ccctgatcgg cggctccggc gttccgtacg
     4321 cgggctcgat gacgtaccac ctgatggaat ccttcgcgat caacgccggc atctgcctgc
     4381 acctgcgcct gctggccgga cgcgacccgc atcacatcgt cgaggcccag ttcaaggccg
     4441 tcgcccgggc gctgcggatc gcgttcgcca tcgacccacg ggttgcggat gccattccgt
     4501 ccacgaaggg cgccctgtga ccgctccccg ggtggtggtg ctcgactacg gctcgggcaa
     4561 tctgcactcc gccacccggg ccctggccgc ggccggtgcc gacgtcacct tgacggcgga
     4621 cagccgcgcg gcgtccgcgg ccgacggcct ggtggtgccc ggcgtcgggg cgttcgcctc
     4681 ctgtatggac ggccttcgcg cggtcggcgg cccgcaactg atcaccgacc ggctggcgcg
     4741 gggacgtccc gtgctcggca tttgcgtcgg ccaccaggtg atgttcggcc acggcgtcga
     4801 acacggtcac gacgcggcgg gcgtgggcgt gctgcccggg gtggtcgaac ggttgaacag
     4861 cgaccggctg ccgcacatgg gctggaacac catcgaacca ccaccgggca gcaccctgtt
     4921 cgcgggcctg gcgggggagc ggttctactt cgtccattcc tacgcggtgc gaaccgccga
     4981 ccggctggcg gacgacgcgc acgtcacctg ggccgagcac ggcggcgacc ggttcgtcgc
     5041 ggccgtcgag gtggggggcc tgtcgagcac gcagttccac cccgagaagt cgggccgggc
     5101 cggtgcggcc ctgttgagca actggctcgc caccctctga accatcggat ccaccgctgg
     5161 tcgagggtgt cgggccgaga gggctggggg ttacgacaat cgcggccatc ccaggcgccg
     5221 ctggtcgagc gtggcggacc cgatgggggt ggggttgcga tgattgcaac catcggaagc
     5281 gccgctggtc gagcgtgtcg gacccgatgg gccgaggatg cagcgaccaa gccggcccgg
     5341 tcatgccacg tattgaccgc caccaggcag cgacgaagac tggccgcatg accgatctca
     5401 gcgcagcgct cactgcggcg gccgaggagc aggcattcag tggtgtggtg cgggtcgacg
     5461 acgccggcac cgtcaccgaa ctcgccttcg gcctggccga ccgggcgc
//
LOCUS       DAOSUG010000157         5499 bp    DNA     linear   ENV 10-JUN-2024
DEFINITION  MAG TPA_asm: Micropruina sp. isolate AMR_MDS_2195
            NODE_6695_length_5499_cov_3.946547, whole genome shotgun sequence.
ACCESSION   DAOSUG010000157 DAOSUG010000000
VERSION     DAOSUG010000157.1
DBLINK      BioProject: PRJNA925477
            BioSample: SAMN32864262
            Sequence Read Archive: SRR4236673
KEYWORDS    WGS; ENV; Metagenome Assembled Genome; MAG; Third Party Data; TPA;
            TPA:assembly.
SOURCE      Micropruina sp. (wastewater metagenome)
  ORGANISM  Micropruina sp.
            Bacteria; Bacillati; Actinomycetota; Actinomycetes;
            Propionibacteriales; Nocardioidaceae; Micropruina.
REFERENCE   1  (bases 1 to 5499)
  AUTHORS   Abdulkadir,N., Saraiva,J.P., Zhang,J., Stolte,S., Gillor,O.,
            Harms,H. and Rocha,U.
  TITLE     Genome-centric analyses of 165 metagenomes show that mobile genetic
            elements are crucial for the transmission of antimicrobial
            resistance genes to pathogens in activated sludge and wastewater
  JOURNAL   Microbiol Spectr 12 (3), e0291823 (2024)
   PUBMED   38289113
REFERENCE   2  (bases 1 to 5499)
  AUTHORS   Abdulkadir,N.
  TITLE     Direct Submission
  JOURNAL   Submitted (24-MAR-2023) Environmental Microbiology, Helmholtz
            Center for Environmental Research -UFZ, Permoserstr 15, Leipzig
            04318, Germany
COMMENT     The annotation was added by the NCBI Prokaryotic Genome Annotation
            Pipeline (PGAP). Information about PGAP can be found here:
            https://www.ncbi.nlm.nih.gov/genome/annotation_prok/
            
            ##Genome-Assembly-Data-START##
            Assembly Method        :: SPAdes v. 3.11.1
            Genome Representation  :: Full
            Expected Final Version :: Yes
            Genome Coverage        :: Not Applicable
            Sequencing Technology  :: Illumina MiSeq
            ##Genome-Assembly-Data-END##
            
            ##Genome-Annotation-Data-START##
            Annotation Provider               :: NCBI
            Annotation Date                   :: 05/04/2023 04:57:10
            Annotation Pipeline               :: NCBI Prokaryotic Genome
                                                 Annotation Pipeline (PGAP)
            Annotation Method                 :: Best-placed reference protein
                                                 set; GeneMarkS-2+
            Annotation Software revision      :: 6.5
            Features Annotated                :: Gene; CDS; rRNA; tRNA; ncRNA
            Genes (total)                     :: 2,776
            CDSs (total)                      :: 2,750
            Genes (coding)                    :: 2,714
            CDSs (with protein)               :: 2,714
            Genes (RNA)                       :: 26
            tRNAs                             :: 24
            ncRNAs                            :: 2
            Pseudo Genes (total)              :: 36
            CDSs (without protein)            :: 36
            Pseudo Genes (ambiguous residues) :: 0 of 36
            Pseudo Genes (frameshifted)       :: 2 of 36
            Pseudo Genes (incomplete)         :: 32 of 36
            Pseudo Genes (internal stop)      :: 2 of 36
            ##Genome-Annotation-Data-END##
FEATURES             Location/Qualifiers
     source          1..5499
                     /organism="Micropruina sp."
                     /mol_type="genomic DNA"
                     /submitter_seqid="NODE_6695_length_5499_cov_3.946547"
                     /isolate="AMR_MDS_2195"
                     /isolation_source="Activated sludge of municipal
                     wastewater treatment plants"
                     /db_xref="taxon:2737536"
                     /environmental_sample
                     /geo_loc_name="Canada"
                     /lat_lon="49.25 N 123.1 W"
                     /collection_date="2015-06-16"
                     /metagenome_source="wastewater metagenome"
                     /note="metagenomic"
     gene            <1..>405
                     /locus_tag="PLF56_06455"
     CDS             <1..>405
                     /locus_tag="PLF56_06455"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_008355047.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_function: GO:0022857 - transmembrane transporter
                     activity [Evidence IEA];
                     GO_process: GO:0055085 - transmembrane transport [Evidence
                     IEA]"
                     /codon_start=2
                     /transl_table=11
                     /product="MFS transporter"
                     /protein_id="HOQ53239.1"
                     /translation="FAGQVLLVLIPASDGVAQPSTYALLGVCLLLDAFGAGMLFMLSE
                     SLVALHVDKHERSRVMAIQRTCVMLATAPFGWISGWLSAMDRRLPFVLTAVLLGIGAV
                     VAARFWVTTHSDQEGEPMPIAPVRSSTSTTRA"
     assembly_gap    406..415
                     /estimated_length=10
                     /gap_type="within scaffold"
                     /linkage_evidence="paired-ends"
     gene            526..672
                     /locus_tag="PLF56_06460"
     CDS             526..672
                     /locus_tag="PLF56_06460"
                     /inference="COORDINATES: ab initio
                     prediction:GeneMarkS-2+"
                     /note="Derived by automated computational analysis using
                     gene prediction method: GeneMarkS-2+."
                     /codon_start=1
                     /transl_table=11
                     /product="hypothetical protein"
                     /protein_id="HOQ53240.1"
                     /translation="MTWVLYAVGFLADLAAWAAVSAIPLLVLDGWMGGWRRCCCSSWW
                     SRRP"
     gene            complement(669..2801)
                     /locus_tag="PLF56_06465"
     CDS             complement(669..2801)
                     /locus_tag="PLF56_06465"
                     /inference="COORDINATES: ab initio
                     prediction:GeneMarkS-2+"
                     /note="Derived by automated computational analysis using
                     gene prediction method: GeneMarkS-2+."
                     /codon_start=1
                     /transl_table=11
                     /product="hypothetical protein"
                     /protein_id="HOQ53241.1"
                     /translation="MPRLDDPSIREPQNGIALVNGPGASPGELKTLPLPPGEAPASGQ
                     YVVHDTDLVHTPYLPDVLARGLGFVFPDAGKEFSIVPPFGVEGFTARFEGVLATPDAS
                     SAWPAIDGYRLVLDGGTELNGRVDAHLITISLPHGTALRAKVSSSFTLDDLSLMGPWL
                     QLPEGIRQLPGVAQAAANGWLWPLTPPSALTLVHAVPRPIAAPEPIGFTIYRAASATN
                     VMLLGGVELHGPSTDSLIVDARWDEVLDDLSDPQWKTLPNQRQVAAQTAIEPWEDIAV
                     LAGGHADQTYNDAVVRHVRSHQAVHELGDTKHRLVNYRFRATTRFREYFAPDELAPLP
                     PSDDPALPIDDGRSTVSAEVALHVPNTSLPAPPMVHSVIPLFRWEEQTEPDQPMARRR
                     TRRSGVRVYLERPWFVTGADEQIGVVLAVGARPETTFTSQWGGDPFWAGSNVADREAV
                     QVTDLLGAIGLDDYDSVAGPSGKPQVYPSAEDPNVKVMVIPYRPQYNLARRKWFVDIG
                     FPARFWPFVQLSLVRYQPYSVDGRHISKPVRCDFVQIVPERLASVSRVSEGRVRVLVS
                     GPAGYHDGNAEAVGARAVSMVGLTAANRALAGVAAVGANRVLRAKLQTRDVGATSDLA
                     WTTVTQTDLMVRGYAGSLANLVWTGELDVPEGLPFARPTTPGTHRVRIEEWETFVGDP
                     VDLGNPHSEPTRENRLVYADEFELGSVD"
     gene            complement(2714..>3567)
                     /locus_tag="PLF56_06470"
     CDS             complement(2714..>3567)
                     /locus_tag="PLF56_06470"
                     /inference="COORDINATES: ab initio
                     prediction:GeneMarkS-2+"
                     /note="Derived by automated computational analysis using
                     gene prediction method: GeneMarkS-2+."
                     /codon_start=3
                     /transl_table=11
                     /product="hypothetical protein"
                     /protein_id="HOQ53242.1"
                     /translation="PQGAPGEGVAAEQLVAVQTVDLGALREFRAGALSRLRERPIEAE
                     AAVQTIEPGQGEAGLQLAEVLPRIVGRLQVKRGEMTEQAPSALAEVRSALASGLADDA
                     LAPAATAVLNPAVIASGLALDRDDLAGPIGAAEALAVSAELPFVRWQPVPPPAIVPRR
                     LFTEGESNQVLVIRSWVSQDPSSLVVTSGTPADYAAANAGRDATCERHLVPPKTSQLG
                     PRSTAHSTPRSPRRTPPPATCGCTRRSASRARCSTWMCRGWTIRPSVSRRTASRWSTG
                     RARPRAS"
     assembly_gap    3568..3667
                     /estimated_length=100
                     /gap_type="within scaffold"
                     /linkage_evidence="paired-ends"
     gene            complement(<3668..>5499)
                     /locus_tag="PLF56_06475"
     CDS             complement(<3668..>5499)
                     /locus_tag="PLF56_06475"
                     /inference="COORDINATES: ab initio
                     prediction:GeneMarkS-2+"
                     /note="Derived by automated computational analysis using
                     gene prediction method: GeneMarkS-2+."
                     /codon_start=2
                     /transl_table=11
                     /product="hypothetical protein"
                     /protein_id="HOQ53243.1"
                     /translation="PYSCSADDPFHVSLFISPDIEVDDPRDTLAVTELFQHWTKALAE
                     ARFVLEDQSGELPCEPLLGAIEDGLWEEVFPAETPVNSYTPPEWTQRPWRTFHPGATT
                     GIARMVNLLGTMVNGVDAPTVQQLPWYGTDIFTTLRGGEAGRQRFNPLDLVSRFETSL
                     TEALDEATRERVPLAEFGDPREYFANGSVSDNVLGGATALGPSLVELHRARRFYSHPE
                     EETTYQRRPDPDNPGPPPPDKQIPDFHARVSLVNDHGPLQRKLGLVVDVRSDPDRLAT
                     SEWLLGRILIPNREDATKVVRVATRRVGRALVTRAENESQWINGRLPVGNVDSFDLLD
                     LDPDGAALKTEQYLSSYPRTVAAALQGAPANAAPPALRTTGFTVARKNNGATTAGAMG
                     RQTSVLDGVRNQPPMLPAPDAPLLTTEDVMQGVRVEVHDSTTDLWRSLHQVHVKADVV
                     GGRSLEFDDIAFLQESAPTHAGADKSPIHVHEAVFGWDGWSLSAARPALPVVNEGGVE
                     RVVTDPQPPADPSTPVLIDRTAAPGTLPRLRFGRRYSFRVWGVDLACNSRPHRFGKAA
                     RQGPNGGGQPPEGQQPQGAPGEVQQPQGAPGEVQQPQGAPGEVQ"
BASE COUNT          734 a         1896 c         1941 g          818 t
ORIGIN      
        1 cttcgccggg caggtgctgt tggtgctgat cccggcatcg gacggggtcg cgcagccgtc
       61 cacctacgcg ttgctggggg tgtgcctgct gctggacgcg ttcggcgccg gcatgctctt
      121 catgctgtcg gagtcgctgg tcgcactgca cgtcgacaag cacgaacgct cccgagtgat
      181 ggccatccag cgcacctgcg tgatgctggc gaccgcaccg ttcggctgga tctcgggctg
      241 gctctcggcc atggaccggc ggctgccgtt cgtgctgacc gcggtgctgc tgggcatcgg
      301 tgcggtggtc gcggcacggt tctgggtgac cacgcactcc gatcaggagg gcgagccgat
      361 gcccatcgcc ccggtcaggt cgagcacttc gacgacgcgg gcgtannnnn nnnnnatcgg
      421 atggcgccgg cgtgtcgtcg atgtggggtg acattgcgtc ctctgacgga ggtccgcgaa
      481 cgggaagagg acagacccgt tccgttcatg cgtagcctgt ggcccgtgac gtgggtgctg
      541 tatgccgtcg gctttctcgc cgacctcgcc gcgtgggcgg cggtctcggc gattccattg
      601 ttggtgctgg acggctggat gggtggctgg cggcggtgct gctgttcgtc ctggtggtca
      661 cggcggccct agtccacgct gcccagctcg aactcatcgg cgtagaccag ccggttctcc
      721 ctggtcggtt cgctgtgcgg gttgcccagg tcgacggggt cgccgacgaa ggtctcccac
      781 tcttcgatcc ggacgcggtg cgtacccggc gtggtcggac gggcgaacgg caggccctcg
      841 ggtacgtcca gttcgccggt ccagaccagg ttggccaacg agccggcgta accgcgcacc
      901 atcaggtcgg tctgggtcac cgtcgtccag gcgagatcgc tggtcgcacc cacgtctcgg
      961 gtctgaagct tggcccgcaa cacccggttg gcgcccaccg ctgcgacccc ggccagggcc
     1021 cggttcgccg cggtcagccc gaccatgctg acggcccggg cccccacggc ctcggcattt
     1081 ccgtcgtggt acccggccgg gcctgagacc agcacccgca cccggccttc gctgacgcga
     1141 ctcaccgacg ccagccgctc cggcacgatc tgcacgaagt cgcagcgcac cggcttgctg
     1201 atgtgccggc cgtccacgct gtagggctgg tagcgcacca gcgacagctg cacgaacggc
     1261 cagaaccggg ccgggaagcc gatgtcgacg aaccacttgc gccgcgccag gttgtactgc
     1321 ggacgatacg gaatgaccat caccttcacg ttcggatcct ccgccgacgg gtagacctgc
     1381 ggctttccgg acggtcccgc gaccgagtcg tagtcgtcca gcccgatcgc gcccagcagg
     1441 tcggtgacct ggacggcctc gcggtcggcg acgttgctgc cggcccagaa cgggtcgccg
     1501 ccccactgac tggtgaacgt cgtctcggga cgggccccga ccgccagcac caccccgatc
     1561 tgctcgtcgg cgccggtgac gaaccacggc cgttccaggt acacccgcac gcccgagcgc
     1621 cgggtgcgcc ggcgggccat cggctgatcg ggttcggtct gctcctccca gcggaacagc
     1681 gggatcaccg agtgcaccat cggcggcgcc ggcagcgagg tgttcggcac gtgcagcgcg
     1741 acctcggcgc tgacggtgct gcgcccgtcg tcgatcggca acgccgggtc gtcggacggc
     1801 ggcagcggcg ccagttcgtc cggcgcgaag tactcgcgga accgggtggt ggcccggaac
     1861 cggtagttca ccagccggtg tttggtgtcg ccgagctcgt gcaccgcctg gtgcgagcgc
     1921 acatgtcgca caaccgcgtc attgtaggtc tggtcggcat gcccgccggc cagcaccgcg
     1981 atgtcctccc acggctcgat ggcggtctgg gccgccacct gccgctggtt gggcagcgtc
     2041 ttccactgcg gatcggacag gtcgtcgagc acctcgtccc agcgggcgtc gacgatcagc
     2101 gagtcggtgc tcggcccgtg cagttccacc ccgccgagca gcatcacgtt ggtggcgctc
     2161 gccgcccggt agatggtgaa accgatcggt tccggcgccg cgatcggacg gggcaccgca
     2221 tgcaccaacg tcagcgcgga cggcggggtc agcggccaca gccatccgtt ggcggccgcc
     2281 tgggcgacgc cgggcaattg ccggatgccc tccggcagtt gcagccacgg acccatcagg
     2341 ctcaggtcgt ccagcgtgaa cgacgacgac accttggccc gcagcgccgt gccgtgcggc
     2401 agcgagatcg tgatcaggtg cgcgtcgacc cggccgttca gctcggttcc cccgtcgagc
     2461 accaaccggt agccgtcgat cgccggccac gccgacgagg catcgggcgt agccaagacc
     2521 ccctcgaagc gcgcggtgaa gccctcgacg ccgaacggcg gcacgatcga gaactccttg
     2581 ccggcatcgg gaaagacgaa gcccaggccg cgggcgagca cgtccggcag gtagggggtg
     2641 tgcaccaggt cggtgtcgtg caccacgtac tgacccgagg ccggcgcctc accgggcggc
     2701 aacggcagcg tcttcagctc gcccggggac gcgcccggcc cgttgaccag cgcgatgccg
     2761 ttctgcggct cacggatgga cggatcgtcc agccgcggca catccaggtc gaacagcgtg
     2821 cccgactcgc ggatcgccgc gtgcagccac aggttgcggg cgggggcgtc cggcgaggcg
     2881 atcgcggcgt cgaatgcgcc gtgctcctcg gccccagctg gctggtcttg ggcggcacca
     2941 ggtggcgctc gcaggttgcg tcgcggcccg cgttggcagc ggcgtagtcg gccggcgttc
     3001 ccgaggtcac caccagcgag gacggatcct ggctcaccca cgagcggatc accagcacct
     3061 ggttcgactc gccctcggtg aacaaccggc gcggcacgat cgcgggcggt ggcacgggtt
     3121 gccagcgcac gaacggcaac tcggccgaga ccgcgagcgc ctcggcggcg ccgatcgggc
     3181 cggccaggtc gtcgcgatcc agggcgagtc cggacgcgat cacggccggg ttcagcacgg
     3241 ccgtcgcggc gggcgccagg gcgtcgtcgg ccaggccgct ggccagggcg ctgcgcactt
     3301 cggccagcgc ggacggtgcc tgttcggtca tctcgccgcg cttgacctgc agccgaccga
     3361 cgatccgcgg cagcacctcc gccagttgca ggccggcctc gccctggccg ggctcgatgg
     3421 tctggacggc cgcctcggcc tcgatcggac gctcgcgcag ccggctgagc gcaccggcgc
     3481 ggaactcccg cagggcaccc aggtcgacgg tctgcaccgc aacgagctgt tcggccgcga
     3541 ccccctcgcc cggtgcaccc tggggctnnn nnnnnnnnnn nnnnnnnnnn nnnnnnnnnn
     3601 nnnnnnnnnn nnnnnnnnnn nnnnnnnnnn nnnnnnnnnn nnnnnnnnnn nnnnnnnnnn
     3661 nnnnnnngct ggacctcgcc cggtgcaccc tggggctgct ggacctcgcc cggtgcaccc
     3721 tggggctgct ggacctcgcc cggtgcaccc tgaggttgct ggccctcggg cggttgcccg
     3781 ccaccgttcg gtccctgccg cgcagccttg ccgaaccgat gcggccgcga attgcaggcc
     3841 aggtcgacac cccacacccg gaagctgtac cggcgtccga accgcagccg gggcagggtg
     3901 ccgggcgcgg ccgtccggtc gatcaacacc ggcgtcgacg ggtccgcggg cggctgcggg
     3961 tcggtgacga ctcgttcgac gccgccctcg ttgaccaccg gcagggcggg ccgggccgcg
     4021 gacagactcc agccgtccca gccgaacacc gcctcgtgca cgtggatcgg cgatttgtcg
     4081 gcaccggcat gcgtgggcgc cgactcctgc agaaaggcga tgtcgtcgaa ctcgagcgag
     4141 cgcccgccga ccacgtcggc cttcacgtgc acctgatgca gcgagcgcca gagatcggtc
     4201 gtcgaatcgt gcacttcgac ccggacgccc tgcatcacgt cctcggtggt gagcagcggg
     4261 gcgtccggcg cgggcagcat ggggggctgg ttgcgcaccc cgtccagcac cgaggtctgc
     4321 cgacccatcg cgcccgctgt ggtggcgcca ttgttcttgc gggccacggt gaacccggtg
     4381 gtgcgcagcg ccgggggagc ggcgttggcg ggcgcgccct gcagcgccgc cgcgaccgtg
     4441 cgcgggtagc tcgacaggta ctgctcggtc ttcaacgcgg cgccgtcggg gtcgaggtcg
     4501 agcaggtcga acgagtcgac gttgccgacg ggcagccgtc cgttgatcca ctgcgactcg
     4561 ttctcggccc gggtgaccag cgcacgcccg acccgccggg tggcgacccg gacgaccttc
     4621 gtggcgtcct cgcggttcgg aatcaggatg cggcccagca gccactcgct ggtcgccagc
     4681 cggtcgggat cggagcgcac gtcgaccacc aggcccagct tgcgctgcaa cggtccgtga
     4741 tcgttcacca gcgacacccg ggcgtgaaag tccggaatct gcttgtcggg cggcggtggg
     4801 ccggggttgt cggggtccgg acgccgctgg tacgtcgtct cctcctcggg atgggagtag
     4861 aaccgccgcg cccggtgcag ttcgaccagg gacggaccga gggccgtcgc gccgcccagc
     4921 acgttgtcgg agacggaacc gttcgcgaag tactcccgcg ggtcgccgaa ctcggccagc
     4981 ggcacgcgct cgcgggtcgc ctcgtccagc gcctcggtga ggctggtctc gaaccgcgac
     5041 accaggtcga gcgggttgaa ccgctgccga cccgcctcgc cgccgcgcag ggtggtgaag
     5101 atgtcggtgc cgtaccaggg cagttgctgc acggtggggg cgtccacgcc gttcaccatg
     5161 gtgcccagca ggttgaccat gcgggcgatg ccggtggtgg cgcccgggtg aaaggtacgc
     5221 cagggacgct gcgtccactc cggaggggtg taggagttca ccggggtctc ggccgggaag
     5281 acctcctccc acaggccgtc ctcgatcgcc ccgagcaggg gttcgcaggg cagttcgccg
     5341 ctctggtcct cgagcacgaa cctggcctcg gcaagggctt tcgtccagtg ctggaacagc
     5401 tcggtgaccg cgagggtgtc gcgtggatcg tccacctcga tgtcgggcga gatgaagagg
     5461 gagacgtgga agggatcgtc cgcgctgcac gagtagggc
//
LOCUS       DAOSUG010000158         5497 bp    DNA     linear   ENV 10-JUN-2024
DEFINITION  MAG TPA_asm: Micropruina sp. isolate AMR_MDS_2195
            NODE_6701_length_5497_cov_3.725469, whole genome shotgun sequence.
ACCESSION   DAOSUG010000158 DAOSUG010000000
VERSION     DAOSUG010000158.1
DBLINK      BioProject: PRJNA925477
            BioSample: SAMN32864262
            Sequence Read Archive: SRR4236673
KEYWORDS    WGS; ENV; Metagenome Assembled Genome; MAG; Third Party Data; TPA;
            TPA:assembly.
SOURCE      Micropruina sp. (wastewater metagenome)
  ORGANISM  Micropruina sp.
            Bacteria; Bacillati; Actinomycetota; Actinomycetes;
            Propionibacteriales; Nocardioidaceae; Micropruina.
REFERENCE   1  (bases 1 to 5497)
  AUTHORS   Abdulkadir,N., Saraiva,J.P., Zhang,J., Stolte,S., Gillor,O.,
            Harms,H. and Rocha,U.
  TITLE     Genome-centric analyses of 165 metagenomes show that mobile genetic
            elements are crucial for the transmission of antimicrobial
            resistance genes to pathogens in activated sludge and wastewater
  JOURNAL   Microbiol Spectr 12 (3), e0291823 (2024)
   PUBMED   38289113
REFERENCE   2  (bases 1 to 5497)
  AUTHORS   Abdulkadir,N.
  TITLE     Direct Submission
  JOURNAL   Submitted (24-MAR-2023) Environmental Microbiology, Helmholtz
            Center for Environmental Research -UFZ, Permoserstr 15, Leipzig
            04318, Germany
COMMENT     The annotation was added by the NCBI Prokaryotic Genome Annotation
            Pipeline (PGAP). Information about PGAP can be found here:
            https://www.ncbi.nlm.nih.gov/genome/annotation_prok/
            
            ##Genome-Assembly-Data-START##
            Assembly Method        :: SPAdes v. 3.11.1
            Genome Representation  :: Full
            Expected Final Version :: Yes
            Genome Coverage        :: Not Applicable
            Sequencing Technology  :: Illumina MiSeq
            ##Genome-Assembly-Data-END##
            
            ##Genome-Annotation-Data-START##
            Annotation Provider               :: NCBI
            Annotation Date                   :: 05/04/2023 04:57:10
            Annotation Pipeline               :: NCBI Prokaryotic Genome
                                                 Annotation Pipeline (PGAP)
            Annotation Method                 :: Best-placed reference protein
                                                 set; GeneMarkS-2+
            Annotation Software revision      :: 6.5
            Features Annotated                :: Gene; CDS; rRNA; tRNA; ncRNA
            Genes (total)                     :: 2,776
            CDSs (total)                      :: 2,750
            Genes (coding)                    :: 2,714
            CDSs (with protein)               :: 2,714
            Genes (RNA)                       :: 26
            tRNAs                             :: 24
            ncRNAs                            :: 2
            Pseudo Genes (total)              :: 36
            CDSs (without protein)            :: 36
            Pseudo Genes (ambiguous residues) :: 0 of 36
            Pseudo Genes (frameshifted)       :: 2 of 36
            Pseudo Genes (incomplete)         :: 32 of 36
            Pseudo Genes (internal stop)      :: 2 of 36
            ##Genome-Annotation-Data-END##
FEATURES             Location/Qualifiers
     source          1..5497
                     /organism="Micropruina sp."
                     /mol_type="genomic DNA"
                     /submitter_seqid="NODE_6701_length_5497_cov_3.725469"
                     /isolate="AMR_MDS_2195"
                     /isolation_source="Activated sludge of municipal
                     wastewater treatment plants"
                     /db_xref="taxon:2737536"
                     /environmental_sample
                     /geo_loc_name="Canada"
                     /lat_lon="49.25 N 123.1 W"
                     /collection_date="2015-06-16"
                     /metagenome_source="wastewater metagenome"
                     /note="metagenomic"
     gene            79..2550
                     /locus_tag="PLF56_06480"
     CDS             79..2550
                     /locus_tag="PLF56_06480"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_013160352.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="DUF3516 domain-containing protein"
                     /protein_id="HOQ53244.1"
                     /translation="MYEAFVDRAAADGLALYPHQDEAIIDVLSGDNVIVTTPTGSGKS
                     LIATAAHFACVAGGGRSYYSAPIKALVSEKFFALCAIFGAERVGMVTGDAAVNADAPI
                     ICCTAEILANLALREGRAARVDQVIMDEFHFLADRDRGWAWQVALVELPQAQFVLMSA
                     TLGDMTQIAAGLERSTGRGVSIVGGAVRPVPLVFSWATTPVHETIQHLIDTHQAPVYV
                     VHATQSGAVDRAQSLISSGIVDRTHRSELQAALRGFRFSGGFGKTLSTLLHAGIAVHH
                     AGMLPRYRRLVETLAQRGLLTVICGTDTLGVGINVPIRTVLFASLAKFDGRRSRVLRS
                     REFHQISGRAGRPGFDSVGYVVAQAPDHEVENTRILAKFADDPKKLKSVRRKKPPEGV
                     VNYTEATFTRLIDSSPEPLHARMRITHAMLLNLLQRDEDTGAALVSLVDSCTVDRPTR
                     QRMLRRAAAMARSLLRAGVVVRLPEPTAGGRRYALAVDLQHNFALNQPLSAFALAVMV
                     QLDPDAPTHPLDVVSVIEATLDDPMVILLAQQFKARGETVAELKAEGYDFDERQEILD
                     EVTWPRPLAELLEEEYLRELPQHPWLAETPLRPKSVVRDLFERAMTFGEYVAFYKLQR
                     SEGLLLRYLSDAYRALRQTVPEGLRTDDLEDLIEWLGALTRMVDSSLLDEWESLAGLS
                     IAPLTDEPGEVRPATRTLTSNVRAFRVLVRNAMFRRVELAAADDVDALAALEPGSELG
                     RRYWDEALGEYWDEHDLIGIGPDARGPALLVIADQGRAWQVRQIIDDPDGNHDWSITA
                     TIDLDECDEAGELVVHTLGFARLDA"
     gene            2850..3485
                     /locus_tag="PLF56_06485"
     CDS             2850..3485
                     /locus_tag="PLF56_06485"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_020575348.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_function: GO:0008703 -
                     5-amino-6-(5-phosphoribosylamino)uracil reductase activity
                     [Evidence IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="dihydrofolate reductase family protein"
                     /protein_id="HOQ53245.1"
                     /translation="MSSTRVHNFSISLDGFATGEPQSLEAPFGHAGHRLHDWMFATRF
                     WDASGSAGVDDAFAQRTEDGIGAEIMGAHKFGPPGWQDDPGWTGWWGEEPPFHTPTFV
                     LTHRLRPAIELKGGTTFHFIDATPQAALALAREAAEGRDVRIGGGPTVVRDFLSAGLI
                     DHLHLVQVPIVLGRGVRLWDGLEGLQNDYAVEAVSSPSGVTHLTFTRRVRS"
     gene            complement(3832..4347)
                     /locus_tag="PLF56_06490"
     CDS             complement(3832..4347)
                     /locus_tag="PLF56_06490"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_008356778.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_function: GO:0003723 - RNA binding [Evidence IEA];
                     GO_function: GO:0008173 - RNA methyltransferase activity
                     [Evidence IEA];
                     GO_process: GO:0006396 - RNA processing [Evidence IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="TrmH family RNA methyltransferase"
                     /protein_id="HOQ53246.1"
                     /translation="MSRRVTTPADLRRGRRRTQHGCWGHLIVAPLWVAYDANLGTLQR
                     TCDAVGACLAVPDTPHYRDAINQGNTLGRRGPCLHRVFDKDSWVRSMHSQGWCIVAVE
                     LAEGAIPLPRLQPARRPTIVLLGHEHHGVPHSMVDLADECVEIPMCGVGASLNVAVAG
                     SLVLYRLAGLS"
     gene            complement(4543..4902)
                     /locus_tag="PLF56_06495"
     CDS             complement(4543..4902)
                     /locus_tag="PLF56_06495"
                     /inference="COORDINATES: ab initio
                     prediction:GeneMarkS-2+"
                     /note="Derived by automated computational analysis using
                     gene prediction method: GeneMarkS-2+."
                     /codon_start=1
                     /transl_table=11
                     /product="hypothetical protein"
                     /protein_id="HOQ53247.1"
                     /translation="MPMTAHDFLSVLTSKVPETQHLVTEHLAHNDELLLHLLTADLLR
                     YAIEEFDSGHRDKLGKLLDVIESALRDGTEYVTNAMAVSFVEDTGWWDPQMQPFIDAW
                     PSELRAEVERQRRSRTA"
     gene            5290..>5497
                     /locus_tag="PLF56_06500"
     CDS             5290..>5497
                     /locus_tag="PLF56_06500"
                     /inference="COORDINATES: ab initio
                     prediction:GeneMarkS-2+"
                     /note="Derived by automated computational analysis using
                     gene prediction method: GeneMarkS-2+."
                     /codon_start=1
                     /transl_table=11
                     /product="hypothetical protein"
                     /protein_id="HOQ53248.1"
                     /translation="MDAGVSEWVWLYHSLDSTVAFVAFGGVMSALPVSVVPTAGDVEL
                     ARDALRLVDGALAESTSDDPVTITV"
BASE COUNT          858 a         1815 c         1896 g          928 t
ORIGIN      
        1 gccgcccccg accccaggac ctcggaaccc gccggcccga ccgccgccca cccgaccgcc
       61 gcccttgacc ccgacgccct gtacgaggcg ttcgtcgacc gggccgcggc cgacggcctc
      121 gctctgtacc cgcatcagga cgaggcgatc atcgacgtgc tgtccgggga caacgtgatc
      181 gtcaccacgc cgaccgggtc cggcaagtcg ctgatcgcga ccgccgcgca cttcgcctgc
      241 gttgccggcg gtggccgcag ctactactcc gcaccgatca aggccctggt cagcgagaag
      301 ttcttcgccc tgtgcgcgat cttcggcgcc gagcgggtag gcatggtgac cggcgacgca
      361 gccgtcaacg ccgacgcccc gatcatctgc tgcacggccg agattctggc caatctcgcg
      421 ctgcgtgagg gacgcgcggc ccgcgtcgac caggtgatca tggacgagtt ccactttctc
      481 gccgaccgcg accgcggctg ggcctggcag gtggccctgg tcgagttgcc gcaggcccag
      541 ttcgtgctga tgtcggccac cctcggcgac atgacccaga tcgccgccgg cctcgagcgc
      601 agcaccgggc gaggggtcag catcgtcggg ggtgcggtgc gtccggtgcc gttggtgttc
      661 tcgtgggcga ccacgccggt gcacgagacc attcagcacc tgatcgacac ccaccaggcg
      721 ccggtgtacg tcgtgcacgc cacccagtcc ggcgccgtcg accgggccca gtcgctgatc
      781 tcgtccggca tcgtcgaccg cactcaccgc agcgagttgc aggcggccct gcgcggcttt
      841 cgcttctcgg gcggcttcgg caagaccctg tcgacgctgt tgcacgcagg catcgcggtg
      901 catcacgcgg gcatgctgcc gcgctaccgc aggctggtcg aaaccctcgc gcagcgcggc
      961 ctgctgacgg tgatctgcgg caccgacacc ctcggcgttg gcatcaacgt gccgatccgc
     1021 accgtgctgt tcgcctcgct ggccaagttc gacggacgcc gctcgcgagt gctgcgcagt
     1081 cgagagttcc accagatctc cggacgcgcc gggcgtccgg gcttcgacag cgtcggctac
     1141 gtggtcgccc aggcgcccga ccacgaggtc gagaacacca ggattctggc caagttcgcc
     1201 gacgacccca agaaactgaa gtcggtgcgg cgcaagaagc cgcccgaggg cgtcgtcaac
     1261 tacaccgagg caaccttcac ccggctgatc gactcatcac ccgaaccgtt gcacgcgcgg
     1321 atgcggatca cccacgccat gctgctcaac ttgttgcagc gcgacgagga caccggtgcc
     1381 gccctggtca gcctggtcga ctcgtgcacc gtcgatcgcc ccacccggca gcgcatgctt
     1441 cgccgagcgg ccgcgatggc gcgctcgtta ctgcgagcgg gggtggtggt gcggctgccc
     1501 gagcccaccg cgggtggacg ccggtacgcg ctggccgtgg acctgcagca caacttcgcc
     1561 ctgaaccagc cgctcagcgc gttcgcgctg gctgtgatgg tgcaactcga cccggacgcg
     1621 cccacccacc cgctcgacgt ggtcagcgtg atcgaggcca ccctggacga cccgatggtg
     1681 atcctgctcg cccagcagtt caaggcccgc ggtgaaacgg tggccgagtt gaaggccgag
     1741 ggctacgact tcgacgagcg gcaagagatt ctggacgagg tgacctggcc ccgtccgctc
     1801 gccgaactgc tggaggagga gtatctgcgc gagctgccgc agcacccgtg gctggccgag
     1861 acgccgcttc ggcccaagtc tgtggtgcgc gacctgttcg agcgggccat gaccttcggc
     1921 gagtatgtgg ccttctacaa gctgcaacgt agcgagggcc tgctgctgcg ctacctctcg
     1981 gacgcctacc gggcgctgcg tcagaccgtg ccggagggcc tgcgcaccga tgacctggaa
     2041 gacctgatcg agtggctggg ggcgttgacc cgcatggtcg actcgtccct gctcgacgag
     2101 tgggagtcat tggcggggct ctcgatcgct cctttgaccg acgaaccggg cgaggtgcgt
     2161 ccggccacca gaacgctgac cagcaacgtc cgggcgtttc gggtgctggt gcgcaacgcg
     2221 atgttccgcc gggtcgaact ggcggccgcc gacgacgtgg acgcgctggc ggccctcgaa
     2281 cccggctcgg agctgggtcg ccggtactgg gacgaggcgt tgggggagta ctgggacgag
     2341 cacgacctga tcggcatcgg cccggacgcc cgcggtccgg ccctgctggt gatcgccgac
     2401 cagggccgcg cctggcaggt gcgccagatc atcgacgatc ccgacggcaa ccacgactgg
     2461 tcgatcacgg caacgatcga cctggacgag tgcgacgagg cgggcgaact ggtggtgcac
     2521 accctggggt tcgcgcggct cgatgcctga ctcgaagacc gcaggcgcgt agggcttcag
     2581 cctcggtgtg ttcagcgtgg ccggcgacga gcacgccgcc gaactgatgt ggcaggtgtg
     2641 gaccggggcg aaagccgacc aggggcgcag tccgtctaca tgctcgaagc ggcggtgagt
     2701 aagccgcggg tgttcgagct gctcaactcg tagcgcgagg gtgcaggtgc gccgcgcggg
     2761 gagccgggcg actcctacgc gctgggcacc accgtgtcgg tccggggcgg tacagtcgct
     2821 gactcgccag cgagcccagg ggagggccca tgtcttcgac ccgcgtgcac aacttctcca
     2881 tctcattgga cggcttcgcc acgggtgagc cgcaatcgct ggaggctccg ttcggccatg
     2941 ccgggcatcg gctgcacgac tggatgttcg ccacccggtt ctgggacgcg tcggggtcgg
     3001 ccggggtgga cgatgccttt gcgcagcgca ccgaggacgg catcggggcc gagatcatgg
     3061 gcgcccacaa gttcggcccg ccggggtggc aggacgatcc ggggtggacg ggctggtggg
     3121 gcgaggagcc tccgttccac accccgacct tcgtgctgac gcatcggctg cggcccgcga
     3181 tcgagctgaa gggcggcacc actttccact tcatcgacgc tacgccgcag gccgctctcg
     3241 cactcgctcg cgaggcggcc gaggggcgcg acgtgcggat cggcggcggc ccgaccgtgg
     3301 tgcgggactt tctatcggcc ggcttgatcg accacctgca tctggtgcag gtgccgatcg
     3361 tgctcggccg cggcgtccgg ctttgggacg gactcgaggg actgcagaac gactacgcag
     3421 tcgaggcggt ctcctcgccc agcggcgtca cccacctgac gttcacccgc agggttcgta
     3481 gctgacctgc gacggcaagg tcgccactaa cgctcgcgat gggcagttcc ggcggcctcg
     3541 ccgtccaacg cgcgcagccg caactggctc cctttcgtga ggcgagccgg ccgctggatc
     3601 gacgcgccgc ttcccgctgt cctcccgggc tgtcatcctg gttgtgagat acctccggat
     3661 ttgctgcggt tcgcgtggca cgtgcaagga tcgatggttc cgccggggac tcggcagccg
     3721 atcgagaagc gtggatggga tgggagtcgc cggaccgtag tcatcctgca gcatgaggtt
     3781 gcgaacctca ccgacggggt gcagtcgggt gcgggcaagg cagatgacgg ttcaggacag
     3841 gccggccagt cggtacagca ccaggcttcc ggcgacggcg acgttcagac ttgcgccgac
     3901 gccgcacatg ggtatctcga cgcactcatc agccagatcg accatgctgt gcgggacgcc
     3961 gtggtgttcg tgacccagca gcacgatggt tggccgccgc gccggctgga gccgtggcag
     4021 gggaattgcg ccctcggcga gctcgacggc gactatgcac catccctgac tgtgcatgga
     4081 cctgacccac gagtccttgt cgaatacgcg atgcaagcac ggcccgcgac ggcccagcgt
     4141 gttgccctgg ttgatggcgt cgcgatagtg cggcgtgtcg ggcactgcca agcaggcgcc
     4201 tacggcgtcg cacgtacgtt gcagggtgcc caggttcgca tcgtaggcaa cccacaaggg
     4261 ggcaacgatg agatgtcccc agcatccgtg ctgggttcgg cggcgaccgc ggcgcaggtc
     4321 ggcgggcgtg gtgacgcgcc tgctcatgcg ggcagggcaa gtccgatcgg gcaagaagtg
     4381 aaggagttca tacatggacg ccattcagcg gcggcgccgg tccatcgacg aaggagggtt
     4441 cttagtgcaa tggtgcgcgc gtggcaagtt gccggtcggg gtacatggcg cgcgatcgct
     4501 ttcggctagg cttctgaacg tccggatccg atgaggggat gactacgcag tccgcgatcg
     4561 ccgttgcctc tcgacctcgg cccgaagctc ggatggccac gcgtcgatga agggctgcat
     4621 ttggggatcc caccagccgg tgtcctccac gaacgacacc gccatggcgt tggtgacgta
     4681 ttccgtcccg tcgcggaggg cgctctcgat gacgtccagc agcttgccca gcttgtctcg
     4741 atgtcccgag tcgaactcct cgatggcgta acgcaggaga tccgcagtta gcaggtgaag
     4801 gaggagttcg tcattgtgcg ccaggtgctc agtcacaaga tgctgagtct cgggcacctt
     4861 gctcgtcagg acggaaagga agtcatgggc agtcattggc accgaagaat catcgcgcac
     4921 tacgtccggt attgccgcgc tccgtgtggt tgtcgtagcg caaaacgggc tagtgctcgg
     4981 ctagctctgc agcacgactg ccggcaatcc cacggcctgc cccatgcgat cccaatccag
     5041 gccgtgctga atgatcgatg cacggttgag gctcgacgtc acatgaaagg cacgcacccg
     5101 agcaacgtag ccatggctga cgcttactac caaccatccg catctgccgc acctcggctg
     5161 tgatcttgtc tgccgcggtg tttggagatt ccgtccggtt ctagtgttgg acccagggtg
     5221 cgctgacgct gaggtaggta ccgtgagttc ggtccccagt gtaccgaggc gggccgaatg
     5281 ggaggagggg tggatgccgg cgtttcggag tgggtctggc tctatcattc acttgattcg
     5341 actgttgcgt ttgttgcgtt tggaggagtg atgagtgctt tgcccgtctc ggtggtcccg
     5401 acagccggcg acgtcgagct tgcccgcgac gcgctgcgtc tggttgacgg tgcgctcgcc
     5461 gagagcacgt ctgacgatcc cgtcaccatc accgtgg
//
LOCUS       DAOSUG010000159         5450 bp    DNA     linear   ENV 10-JUN-2024
DEFINITION  MAG TPA_asm: Micropruina sp. isolate AMR_MDS_2195
            NODE_6761_length_5450_cov_4.376830, whole genome shotgun sequence.
ACCESSION   DAOSUG010000159 DAOSUG010000000
VERSION     DAOSUG010000159.1
DBLINK      BioProject: PRJNA925477
            BioSample: SAMN32864262
            Sequence Read Archive: SRR4236673
KEYWORDS    WGS; ENV; Metagenome Assembled Genome; MAG; Third Party Data; TPA;
            TPA:assembly.
SOURCE      Micropruina sp. (wastewater metagenome)
  ORGANISM  Micropruina sp.
            Bacteria; Bacillati; Actinomycetota; Actinomycetes;
            Propionibacteriales; Nocardioidaceae; Micropruina.
REFERENCE   1  (bases 1 to 5450)
  AUTHORS   Abdulkadir,N., Saraiva,J.P., Zhang,J., Stolte,S., Gillor,O.,
            Harms,H. and Rocha,U.
  TITLE     Genome-centric analyses of 165 metagenomes show that mobile genetic
            elements are crucial for the transmission of antimicrobial
            resistance genes to pathogens in activated sludge and wastewater
  JOURNAL   Microbiol Spectr 12 (3), e0291823 (2024)
   PUBMED   38289113
REFERENCE   2  (bases 1 to 5450)
  AUTHORS   Abdulkadir,N.
  TITLE     Direct Submission
  JOURNAL   Submitted (24-MAR-2023) Environmental Microbiology, Helmholtz
            Center for Environmental Research -UFZ, Permoserstr 15, Leipzig
            04318, Germany
COMMENT     The annotation was added by the NCBI Prokaryotic Genome Annotation
            Pipeline (PGAP). Information about PGAP can be found here:
            https://www.ncbi.nlm.nih.gov/genome/annotation_prok/
            
            ##Genome-Assembly-Data-START##
            Assembly Method        :: SPAdes v. 3.11.1
            Genome Representation  :: Full
            Expected Final Version :: Yes
            Genome Coverage        :: Not Applicable
            Sequencing Technology  :: Illumina MiSeq
            ##Genome-Assembly-Data-END##
            
            ##Genome-Annotation-Data-START##
            Annotation Provider               :: NCBI
            Annotation Date                   :: 05/04/2023 04:57:10
            Annotation Pipeline               :: NCBI Prokaryotic Genome
                                                 Annotation Pipeline (PGAP)
            Annotation Method                 :: Best-placed reference protein
                                                 set; GeneMarkS-2+
            Annotation Software revision      :: 6.5
            Features Annotated                :: Gene; CDS; rRNA; tRNA; ncRNA
            Genes (total)                     :: 2,776
            CDSs (total)                      :: 2,750
            Genes (coding)                    :: 2,714
            CDSs (with protein)               :: 2,714
            Genes (RNA)                       :: 26
            tRNAs                             :: 24
            ncRNAs                            :: 2
            Pseudo Genes (total)              :: 36
            CDSs (without protein)            :: 36
            Pseudo Genes (ambiguous residues) :: 0 of 36
            Pseudo Genes (frameshifted)       :: 2 of 36
            Pseudo Genes (incomplete)         :: 32 of 36
            Pseudo Genes (internal stop)      :: 2 of 36
            ##Genome-Annotation-Data-END##
FEATURES             Location/Qualifiers
     source          1..5450
                     /organism="Micropruina sp."
                     /mol_type="genomic DNA"
                     /submitter_seqid="NODE_6761_length_5450_cov_4.376830"
                     /isolate="AMR_MDS_2195"
                     /isolation_source="Activated sludge of municipal
                     wastewater treatment plants"
                     /db_xref="taxon:2737536"
                     /environmental_sample
                     /geo_loc_name="Canada"
                     /lat_lon="49.25 N 123.1 W"
                     /collection_date="2015-06-16"
                     /metagenome_source="wastewater metagenome"
                     /note="metagenomic"
     gene            complement(<1..618)
                     /locus_tag="PLF56_06505"
     CDS             complement(<1..618)
                     /locus_tag="PLF56_06505"
                     /inference="COORDINATES: ab initio
                     prediction:GeneMarkS-2+"
                     /note="Derived by automated computational analysis using
                     gene prediction method: GeneMarkS-2+."
                     /codon_start=1
                     /transl_table=11
                     /product="hypothetical protein"
                     /protein_id="HOQ53249.1"
                     /translation="MFTSDIVIFLLGLIAAIFGGYFGAAIGGNFAFTLTGFMILFSWG
                     IFAVGGSDIGFNYVAFGPVMGPHITFAAGVAGAAYAMHKGLIESGRDASSPLARLGRL
                     DVLLVGAAFGAFGYLFNIGLSFIPWFGSHIDSVALTVFTSNVLARLIWGNGLLSPQNY
                     NKGATSFMKKIAPNDTYFWLRYQEKPGQYLPLGFFAGGMAAAVSIM"
     gene            complement(664..1155)
                     /locus_tag="PLF56_06510"
     CDS             complement(664..1155)
                     /locus_tag="PLF56_06510"
                     /EC_number="5.3.1.6"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_002531537.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_function: GO:0016853 - isomerase activity [Evidence
                     IEA];
                     GO_process: GO:0005975 - carbohydrate metabolic process
                     [Evidence IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="ribose-5-phosphate isomerase"
                     /protein_id="HOQ53250.1"
                     /translation="MGLRVVVAADIAGYDYKEQLKKDLEADDRVDEVIDVGIGSGEDV
                     DYPHVAVAGARLIAEGKADRGLFVCGTGMGVAIAANKVPGIRASTAHDSFSVERLVLS
                     NNAQVLTFGQRVIGLELARRLAKEWLGYTFDPNSSSAPKVAALESYDTGDNSAEAAQT
                     SCD"
     gene            complement(1296..2045)
                     /locus_tag="PLF56_06515"
     CDS             complement(1296..2045)
                     /locus_tag="PLF56_06515"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_015069874.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_function: GO:0003700 - DNA-binding transcription factor
                     activity [Evidence IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="DeoR/GlpR family DNA-binding transcription
                     regulator"
                     /protein_id="HOQ53251.1"
                     /translation="MTIAERVVKNGSAAVDELVELTGVSAMTVYRDIEALEEAGVLLR
                     HRGQVTAVATGLNEIAASIRVNQNAELKQQVARVVAERIRPGSSIMVDDSTTALWVLR
                     LLEVVPITVVTNSMLVARELERRSGVRLFVTGGEYQAWAEAMLGQTTTDVIAGIRADY
                     CLMSASGISDLQCFHPYEDCVQVKRAMMASSAESILLLDHTKLNRRALHTFASMTDFD
                     QVIVDALTPSDLIADLRERGVNVAQAGAVNE"
     gene            2457..3527
                     /locus_tag="PLF56_06520"
     CDS             2457..3527
                     /locus_tag="PLF56_06520"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_002528362.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="glycerol-3-phosphate dehydrogenase"
                     /protein_id="HOQ53252.1"
                     /translation="MATLVVLGSGIMATALATPFTDNGNEVRLVGTHLDREIIESIKT
                     TGVHPNLNLKVPDGVTAYQLEDAAEAFEGVDLVMSGVNSFGVDWAGEQLAGLLRPGMH
                     VIALAKGMQADEEGNLTILPRALMAHVPESVRDQVSISAIAGPSIAGEVAIRRHTSVV
                     FTGDDPEVLAMWKALYETDFYHVWTNTDFVGVEVCAATKNCYAFGAGFMHGTLNALGL
                     TNPQYVNYNYGAALFGQASVEMTQFMDLLGGQRTTVLGLPGIGDCFVTSMGGRNVKVG
                     TLVGEGLTFSQARDRMPGVTLEGAAAIKVIGDAFERLTERGVVRPEQFPLLRHLHEVV
                     AKDRAVDVPWKTFFGADGSGQG"
     gene            complement(4169..5404)
                     /locus_tag="PLF56_06525"
     CDS             complement(4169..5404)
                     /locus_tag="PLF56_06525"
                     /EC_number="1.1.5.3"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_013161183.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="anaerobic glycerol-3-phosphate dehydrogenase
                     subunit C"
                     /protein_id="HOQ53253.1"
                     /translation="MNASDGLEYAATQLARASLDHCVKCTICETHCPVSAVTDKFTGP
                     KYVGPQAERFRHGESVDHSLDYCSSCGACTLNCPQGVKIAELNGQARAVMKADHMPLR
                     DRLITQTTLMGKAMTPVAPLANAALSIKPLRGIVEKVIGIHRDAPMPRAHTQTITGWL
                     KKRTRTAPPPTRGTLVFFHGCAGGYFEVETSKKSIEVLEHLGYEVLVPKQGCCGLAEQ
                     SNGLFDGATRKVLKLCDELRSSGRDLTIISSSGSCTGMLKHEAHLIMGVDDARLADVG
                     TRIRDIMEFLLELHEAGELPTDFKPIELTAVYHAPCQLKSQGMGQPALEILQLIPGVQ
                     AVESEETCCGITGTYGLKKEKYAIAQAVGQGLFDKVKRVNPEFAMCDTETCRWQIRKG
                     SGAAVEHPIFLVHQAYGLS"
BASE COUNT          932 a         1719 c         1848 g          951 t
ORIGIN      
        1 catgatcgac accgccgcgg ccatgccgcc ggcgaagaag cccagcggca ggtactggcc
       61 cggcttttcc tggtaacgca gccagaagta ggtgtcgttc ggggcgatct tcttcatgaa
      121 cgaggtggcg cccttgttgt agttctgcgg cgacagcagc ccgttgcccc agatcagacg
      181 cgcgagcacg ttcgaggtga acacggtgag ggcgaccgag tcgatgtggc tgccgaacca
      241 cggaatgaag ctcagcccga tgttgaacag gtagccgaac gcgccgaaag cggcaccgac
      301 cagcagcacg tcgagacggc cgagacgggc cagcggcgac gaggcgtctc ggcctgattc
      361 gatcaatccc ttgtgcatgg cgtacgccgc gccggcaacg cctgcggcaa aggtgatgtg
      421 cgggcccatc acggggccga aggccacgta gttgaagccg atgtcgctgc caccgaccgc
      481 gaagatgccc cacgagaaca gaatcatgaa gccggtcagg gtgaacgcga agttgccacc
      541 gatggctgca ccgaagtacc cgccgaagat ggccgcgatc agcccgagca ggaagataac
      601 gatgtcactg gtgaacatgt gccgtccttg tcagattgat tgagggaagg tgagtccagc
      661 tgctcagtcg cagctggttt gcgcggcctc cgcactgttg tcgccggtgt cgtacgactc
      721 gagagcggcc accttgggcg ctgaggacga gttcgggtcg aaggtgtacc cgagccactc
      781 cttggccagt cgcctggcca gttcgagccc gatgacccgc tgaccgaagg tgagcacctg
      841 ggcgttgttc gacaacacga gtcgctcgac ggaaaagctg tcgtgtgccg tactggccct
      901 gatgccggga accttgttgg ctgcgatcgc aacgcccatg ccggtgccgc agacgaacag
      961 gccacggtcg gccttgcctt ctgcgatcag ccgagcgccg gccaccgcca cgtgggggta
     1021 gtcgacgtct tcgccggatc cgatgccaac gtcgatgact tcatcgaccc tgtcgtcggc
     1081 ctcgaggtct ttcttcagtt gctctttgta gtcgtatccg gcgatgtccg ccgcgaccac
     1141 cactcgcagg cccattcgta ctcctttgca cgaggtgacg gacccgccga caggggtccg
     1201 ctggcctgaa cctaccagcg agcaaggtgt ttgtaacaga ctttgtgatg ctttcgagcg
     1261 gtctggaact tatcgggccg ttcagtcgcc cacggtcact cgttgacggc gccggcctgg
     1321 gcgacgttca cgccccgctc gcgcaggtcg gcgatcaggt cggacggcgt cagcgcgtcc
     1381 acgatcacct ggtcgaagtc ggtcatcgag gcgaaggtgt gcagcgcccg acggttcagc
     1441 ttggtgtggt cgagcagcag aatcgactcg gccgacgacg ccatcatggc ccgcttgacc
     1501 tgcacacagt cctcgtaggg gtggaagcac tgcaggtcac tgatgccgga tgccgacatc
     1561 aggcagtagt cggcccggat gccggcgatc acgtcggtgg tggtctggcc gagcatggcc
     1621 tcggcccatg cctggtactc acccccggtg acgaacagcc gcacgccgga gcgccgctcg
     1681 agttcgcggg cgaccagcat cgagttggtc accaccgtga tcgggaccac ctcgagcagc
     1741 cgcagtaccc acagtgcggt ggtggagtcg tccaccatga tcgagctgcc aggcctgatg
     1801 cgctcggcga cgacccgggc cacctgctgc ttgagctcgg cgttctgatt gacgcgaatg
     1861 ctggcggcga tctcgttgag cccggtggcc accgcggtga cctggccacg atgccgcagc
     1921 agcacgcccg cctcttcgag ggcctcaatg tcgcggtaca ccgtcatcgc cgagacgccg
     1981 gtcagctcga cgagttcgtc cacggcggcc gagccgttct tgaccactcg ttcggcgatg
     2041 gtcatgcgcc gttcccgtag cgcgccggca gaaccccggc cacggcccct gactgccacc
     2101 gtcgctcctt tgcatacgtc ccgcgtgtgt gggtaacact atcgtgccgc atgtgagaca
     2161 aaagggcacc cgttgctctc atccaatcgg tgttcgctag cgctgagcac ggcgtgaatc
     2221 acggcatttc ttgcgtggat cgtcaaattt cgctcgtacc gagtacgcga gggttgcccg
     2281 acagaccgct agtcacattc tttgagtggt attcaccgcc gaagaggaca atcacagcgg
     2341 atagcggcta cgttgtgatg atccaggctc ggaggtgccg tcggttgcgt ccgggcattc
     2401 ggccggttcg cggccatcgc atacccggca aggcagccgc acgaaggaga gacgtcatgg
     2461 caacgctcgt agtgctcggt tcaggaatca tggcaaccgc cctggcgacg ccgttcacag
     2521 acaacggcaa cgaggtgcga ctggtcggta cgcacctcga tcgcgagatc atcgagtcga
     2581 tcaagaccac gggtgtgcac ccgaatctga acctgaaggt gccggacggg gtgaccgcct
     2641 accaactcga agacgcggcc gaggctttcg agggcgtcga tctggtgatg agcggagtga
     2701 actcgttcgg cgtcgactgg gccggtgagc agctggccgg cctgctgcgt ccgggcatgc
     2761 acgtgatcgc cctggcgaag ggcatgcagg ccgacgagga gggcaacctg acgatcctgc
     2821 cccgcgcgtt gatggcacac gtgcccgaga gcgtgcgcga ccaggtgtcg atttcggcga
     2881 tcgcggggcc gtccatcgcg ggcgaggtgg cgatccgccg gcacaccagc gtggtattca
     2941 ccggcgacga ccccgaggtg ctggccatgt ggaaggcgtt gtacgagacg gatttctacc
     3001 acgtgtggac gaacaccgac ttcgtcgggg tcgaggtgtg cgcggcgacc aagaactgct
     3061 acgcgttcgg cgcaggtttc atgcacggca cgctgaatgc gttgggcctg accaacccgc
     3121 agtacgtgaa ctacaactac ggtgcggcac tgttcggcca ggcgtcggtc gagatgactc
     3181 agttcatgga tctgttgggt ggacagcgca ccaccgtgct ggggctgccc ggcatcggcg
     3241 actgcttcgt cacctcgatg ggcggccgca acgtcaaggt cggcaccctg gtcggcgaag
     3301 ggctcacctt ctcgcaggcg cgtgaccgca tgcccggcgt caccctcgag ggtgcggccg
     3361 cgatcaaggt gatcggcgat gccttcgagc ggctcaccga gcgcggtgtg gtgcggccgg
     3421 agcagttccc gttgctgcgg cacctgcacg aagtggtggc gaaagaccgg gccgtcgacg
     3481 ttccgtggaa gacgttcttc ggcgcagacg ggtccgggca gggctgaggc cctgctaaac
     3541 gccctccgga agggccccgg agggcgttgt tgtccccgct ggatgcccga ggtctcgacc
     3601 ggcggtggcc ggttgggtct tgaatggctc gaccgccgag gacgcgcggc ccggcagaag
     3661 cgaaagtgtg aagcggagtg agggtgcact ccgtcagggt ggtcgggcgt cgggcaactc
     3721 accggcggag cttgcggagt cgctgaggct gatggtgcgg gcggccgggg tgcaccccac
     3781 tccgcggcag caaacgcaaa agaccgcaca acatcaggcg cggggggaga tcccggcgtc
     3841 cgccgggatg acggtgtgac gctgtgcccc cgttcctgtg ccggtggtga cgcggaaggg
     3901 gtctgttcac tatcccgttc atatgacgag tcgcggcagc gtccacgaac agaagttaag
     3961 cggggtgggg ggagtcggcg cagagacagt acgaagcact tcgtcgttcg ggcggagccc
     4021 ccgacgacga caggaccgtc tcaagccgag ctccccccac cccgcgaccg tgagggacag
     4081 agtccggaag ggacctcgac aagctcgacg aacgacaggt tcgaccgacg gaggggtctc
     4141 gacaggctcg accaacgagg tatcagcgtc agctcagccc gtaggcctgg tgcaccagga
     4201 agatcgggtg ctccaccgcg gcgccgctgc ccttgcggat ctgccagcgg cacgtttcgg
     4261 tgtcgcacat ggcgaactcg gggttgacgc gcttcacctt gtcgaacagc ccttgaccga
     4321 cggcctgggc gatcgcgtac ttctccttct tgagcccgta ggtgcccgtg atgccgcaac
     4381 aagtctcctc ggattcgacc gcctgcaccc ccggaatcag ttgcagaatc tcgagcgccg
     4441 gctgacccat gccctggctc ttcaactggc acggcgcgtg gtagaccgcc gtcagttcga
     4501 tcggcttgaa gtcggtcggt agttcgccgg cttcgtgcag ttcgagcaga aactccatga
     4561 tgtcgcgaat gcgggtgccg acgtcggcga gccgggcgtc gtccacaccc atgatcaggt
     4621 gcgcctcgtg cttcagcatg ccggtgcatg atccggacga gctgatgatg gtcaggtcgc
     4681 gcccggacga gcgcagctca tcgcagagct tgagcacctt gcgggtggcg ccgtcgaaca
     4741 gaccgttgga ctgctccgcc aggccgcagc agccctgctt gggcaccagc acctcatacc
     4801 ccaggtgctc cagcacctcg atcgacttct tcgacgtctc cacctcgaag tagccgccgg
     4861 cgcagccgtg gaagaacacc agggtgccgc gggtgggcgg cggcgccgtc cgggtgcgct
     4921 tcttcagcca gccggtgatg gtctgggtgt gggcgcgcgg catcggggcg tcgcgatgga
     4981 tgccgatcac cttctcgacg atcccgcgca gcggtttgat cgacagggcg gcgttggcca
     5041 gcggggcgac cggagtcatc gccttgccca tcaaggtggt ctgggtgatc agccggtcac
     5101 gcagcggcat gtggtcggcc ttcatcaccg cccgtgcctg gccgttcagc tcggcgatct
     5161 tgaccccctg cgggcagttc agcgtgcacg cgccgcacga gctgcagtag tccagcgagt
     5221 gatcgacgga ctcgccgtgc ctgaaccgct cggcctgcgg cccgacgtac ttgggcccgg
     5281 tgaacttgtc cgtcacggcc gagaccgggc agtgcgtctc gcagatggtg cacttcacgc
     5341 agtgatcgag tgaggcccgc gcgagctgag tggcggcgta ctccaagccg tcggaggcgt
     5401 tcatgaggct gctccttgcg tggtttcggt ggcggccgtg gccagaatcg
//
LOCUS       DAOSUG010000160         5431 bp    DNA     linear   ENV 10-JUN-2024
DEFINITION  MAG TPA_asm: Micropruina sp. isolate AMR_MDS_2195
            NODE_6791_length_5431_cov_4.917039, whole genome shotgun sequence.
ACCESSION   DAOSUG010000160 DAOSUG010000000
VERSION     DAOSUG010000160.1
DBLINK      BioProject: PRJNA925477
            BioSample: SAMN32864262
            Sequence Read Archive: SRR4236673
KEYWORDS    WGS; ENV; Metagenome Assembled Genome; MAG; Third Party Data; TPA;
            TPA:assembly.
SOURCE      Micropruina sp. (wastewater metagenome)
  ORGANISM  Micropruina sp.
            Bacteria; Bacillati; Actinomycetota; Actinomycetes;
            Propionibacteriales; Nocardioidaceae; Micropruina.
REFERENCE   1  (bases 1 to 5431)
  AUTHORS   Abdulkadir,N., Saraiva,J.P., Zhang,J., Stolte,S., Gillor,O.,
            Harms,H. and Rocha,U.
  TITLE     Genome-centric analyses of 165 metagenomes show that mobile genetic
            elements are crucial for the transmission of antimicrobial
            resistance genes to pathogens in activated sludge and wastewater
  JOURNAL   Microbiol Spectr 12 (3), e0291823 (2024)
   PUBMED   38289113
REFERENCE   2  (bases 1 to 5431)
  AUTHORS   Abdulkadir,N.
  TITLE     Direct Submission
  JOURNAL   Submitted (24-MAR-2023) Environmental Microbiology, Helmholtz
            Center for Environmental Research -UFZ, Permoserstr 15, Leipzig
            04318, Germany
COMMENT     The annotation was added by the NCBI Prokaryotic Genome Annotation
            Pipeline (PGAP). Information about PGAP can be found here:
            https://www.ncbi.nlm.nih.gov/genome/annotation_prok/
            
            ##Genome-Assembly-Data-START##
            Assembly Method        :: SPAdes v. 3.11.1
            Genome Representation  :: Full
            Expected Final Version :: Yes
            Genome Coverage        :: Not Applicable
            Sequencing Technology  :: Illumina MiSeq
            ##Genome-Assembly-Data-END##
            
            ##Genome-Annotation-Data-START##
            Annotation Provider               :: NCBI
            Annotation Date                   :: 05/04/2023 04:57:10
            Annotation Pipeline               :: NCBI Prokaryotic Genome
                                                 Annotation Pipeline (PGAP)
            Annotation Method                 :: Best-placed reference protein
                                                 set; GeneMarkS-2+
            Annotation Software revision      :: 6.5
            Features Annotated                :: Gene; CDS; rRNA; tRNA; ncRNA
            Genes (total)                     :: 2,776
            CDSs (total)                      :: 2,750
            Genes (coding)                    :: 2,714
            CDSs (with protein)               :: 2,714
            Genes (RNA)                       :: 26
            tRNAs                             :: 24
            ncRNAs                            :: 2
            Pseudo Genes (total)              :: 36
            CDSs (without protein)            :: 36
            Pseudo Genes (ambiguous residues) :: 0 of 36
            Pseudo Genes (frameshifted)       :: 2 of 36
            Pseudo Genes (incomplete)         :: 32 of 36
            Pseudo Genes (internal stop)      :: 2 of 36
            ##Genome-Annotation-Data-END##
FEATURES             Location/Qualifiers
     source          1..5431
                     /organism="Micropruina sp."
                     /mol_type="genomic DNA"
                     /submitter_seqid="NODE_6791_length_5431_cov_4.917039"
                     /isolate="AMR_MDS_2195"
                     /isolation_source="Activated sludge of municipal
                     wastewater treatment plants"
                     /db_xref="taxon:2737536"
                     /environmental_sample
                     /geo_loc_name="Canada"
                     /lat_lon="49.25 N 123.1 W"
                     /collection_date="2015-06-16"
                     /metagenome_source="wastewater metagenome"
                     /note="metagenomic"
     gene            complement(<1..463)
                     /locus_tag="PLF56_06530"
     CDS             complement(<1..463)
                     /locus_tag="PLF56_06530"
                     /inference="COORDINATES: protein motif:HMM:NF012738.2"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_component: GO:0016020 - membrane [Evidence IEA];
                     GO_process: GO:0055085 - transmembrane transport [Evidence
                     IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="ABC transporter permease subunit"
                     /protein_id="HOQ53254.1"
                     /translation="MTWVLANLDLIGSRTLSHLVLAVPAIVLSFVLALPLGWVANRYR
                     WSRATLLTAAGLLYAIPSLPLFVVLPVIIGIGVRDRLNVIIALTLYGIALMVRSAADA
                     LASVDAPVRLSSTALGYSAAGRFWQVELPLAGPVLLAGLRVVSVSTISLVTV"
     gene            complement(466..1296)
                     /locus_tag="PLF56_06535"
     CDS             complement(466..1296)
                     /locus_tag="PLF56_06535"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_002528913.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_function: GO:0005524 - ATP binding [Evidence IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="ATP-binding cassette domain-containing protein"
                     /protein_id="HOQ53255.1"
                     /translation="MIRFEDVSKSYPDGTQAVQGFSLEAPTGQTTVLVGSSGCGKTTL
                     LRMVNRMVDPSSGIVWIDDENVAEVPPVPLRRRIGYVMQNAGLLPHRKVIDNIATVPL
                     LNGVPKDEAHRRASELMELVGLEARMGRRYPDQLSGGQQQRVGVARGLAADPNILLMD
                     EPFGAVDPLVRADLQAELLRIQRELHKTIVFVTHDIAEALALGDQIVILAKGARIAQA
                     GTPQQVVADPADSFVAEFLGLGRGTLDLHLSETPTGTLVLDAHGRPAGRLVEQPPAGG
                     "
     gene            1407..1691
                     /locus_tag="PLF56_06540"
     CDS             1407..1691
                     /locus_tag="PLF56_06540"
                     /EC_number="2.3.1.-"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_007079482.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_function: GO:0008080 - N-acetyltransferase activity
                     [Evidence IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="GNAT family N-acetyltransferase"
                     /protein_id="HOQ53256.1"
                     /translation="MAETVSHNQRRSRFEIVVDDELAGFAHYDLSNGVATFDHTEVKP
                     AFNGRGLGTTLVAGALDQVRQHGIWKVRATCPFVVTFLRRHPEYADLVVP"
     gene            complement(1711..2442)
                     /locus_tag="PLF56_06545"
     CDS             complement(1711..2442)
                     /locus_tag="PLF56_06545"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_011757994.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="glutamine amidotransferase"
                     /protein_id="HOQ53257.1"
                     /translation="MKPFVLLATRDHDKAARDEYESVRVHAGLAPDELVHLRVEAGPL
                     PRLYLEDYSGVFLGGSPFNISDAVKSDLQLRVESDIRNVVDHIVDADFPFLGMCYGIG
                     TVTVHLGGTVDRTYGEGLGAIEVTLTEAASDDPMMAGIPATFQAFVGHKEACNGAPPG
                     VTVLGTGVACPVQMYRHGSNVYVTQFHPELDIDSLAQRMQIYRNAGYFHPDDFDILIE
                     MARTSGVAEHPHRLLRNFVTRYARD"
     gene            complement(2439..3569)
                     /locus_tag="PLF56_06550"
     CDS             complement(2439..3569)
                     /locus_tag="PLF56_06550"
                     /inference="COORDINATES: protein motif:HMM:TIGR02050.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="glutamate--cysteine ligase"
                     /protein_id="HOQ53258.1"
                     /translation="MRVDFVPSSGPTLGVEWELALVDADSLELVPRAQRLLDAVDDPK
                     RGPIRQEYLTNMIELVTGVHHRVDEAMDDLRESLQRVRAIAGDDTLLLGAGVHPRSPA
                     HDQHHFDTPRYHVVAERNAYWGRLMSIFGTHVHVGIDHRDKALPITYGLARFSPYFIA
                     LTASSPYWEGEDTGYASHRTMLFQQMPTNGLPYPLDSWDEFEHYASELEEAGMITVPS
                     EIRWDVRPSTFGTVENRVLDSVPTFAELGAMAALAQCLATYMSRELDDGRGIARLPMW
                     FMKENKWRAARYGLDTDVVTPRVGQKVRPMREGIMDWLNRLETIAAELHCADELGFIA
                     ELLEHGPSYLRQRAAGGPSAALRQLVAETGAERPSWPLPFSP"
     gene            complement(3637..4482)
                     /locus_tag="PLF56_06555"
     CDS             complement(3637..4482)
                     /locus_tag="PLF56_06555"
                     /inference="COORDINATES: ab initio
                     prediction:GeneMarkS-2+"
                     /note="Derived by automated computational analysis using
                     gene prediction method: GeneMarkS-2+."
                     /codon_start=1
                     /transl_table=11
                     /product="hypothetical protein"
                     /protein_id="HOQ53259.1"
                     /translation="MAARGTATAVLTLVVAAAVTGCTDGASGTQQSPTPRASATTAKA
                     QLPPVKGTVATNKKTPLSLREQPTISSKRLLRIPHKTAITMTCRTIGDTVSNGTHASN
                     VWNKVTYKKKTGYVASVFVNADEAALSLCQAGAVKPTASVTSRPPNVEQAIVKVARGQ
                     RGVEGKKKKCNAYGGCMPWSSLFATWVWNKAGNVVPKFSFSGDLFTWGQKHNRAHEGT
                     AGVGPGDLVLSGTGPTSPKTSTRVDVVVEVLANGKLTVIGGDIDNRVVERTIATKGLY
                     GWVEA"
     gene            complement(4578..>5431)
                     /locus_tag="PLF56_06560"
     CDS             complement(4578..>5431)
                     /locus_tag="PLF56_06560"
                     /inference="COORDINATES: ab initio
                     prediction:GeneMarkS-2+"
                     /note="Derived by automated computational analysis using
                     gene prediction method: GeneMarkS-2+."
                     /codon_start=3
                     /transl_table=11
                     /product="hypothetical protein"
                     /protein_id="HOQ53260.1"
                     /translation="PGVPGSSENGDRFGTALAGQGRAIVIGTPNEAVGTRSNAGSVTM
                     LSATDAAPTTFAGVGVTQNSAGVPGTAESGDRFGEAVAYRDHYVLVGVPGETIGSATY
                     TGQVHVLNFNPATRTVKSLRAVNQNTAGIPGANESGDYFGSSVALGINTLDQLTAIVG
                     APGEAIGSVLGAGSVTMFRANRAGGLTWALRQGADGVPGAPEAADHFGWSVSVLGGNP
                     DDGEAMADGVVIGTPHEDVGSIADAGSVTYTRSTDTWYSLLLEDTGSSAVPADAAYGE
                     TLAGVNG"
BASE COUNT          825 a         1870 c         1865 g          871 t
ORIGIN      
        1 tgaccgtgac caggctgatc gtgctcaccg acaccacgcg caggccggcc agcagcaccg
       61 gaccggccag cggcagttcc acctgccaga accggcccgc cgcggaatag cccagcgccg
      121 tggacgacag ccgcaccggc gcgtcgaccg acgccagggc gtccgcggca gagcgcacca
      181 tgagcgcgat cccgtacagg gtgagcgcga tgatcacgtt gagccggtcg cgcaccccga
      241 tgccgatgat caccggcagc accacgaaca ggggcagcga cggaatcgcg tacagcagcc
      301 cggccgcggt cagcagggtg gcccgcgacc accggtagcg gttggcgacc cagcccagcg
      361 gcagtgcgag cacgaacgac agcacaattg ccggcaccgc cagcaccaga tgcgacagcg
      421 tccgcgaccc gatcaggtcg aggttggcca gcacccaggt catgctcagc cccctgccgg
      481 cggctgctcg accaggcggc ccgccggacg tccgtgggca tcgagcacca gcgtgccggt
      541 cggcgtctcg ctcaggtgca ggtcgagggt gccgcggccg aggccgagaa actcggcgac
      601 gaacgaatcg gccggatcgg ccaccacctg ctgcggcgtg cccgcctgcg cgatccgggc
      661 gcccttggcc aggataacga tctggtcgcc cagtgcgagg gcttcggcga tgtcgtgggt
      721 gacgaacacg atcgtcttgt gcagttcgcg ctggatgcgc agcaactcgg cctggagatc
      781 ggcgcgcacc agcggatcga cggccccgaa cggttcgtcc atcagcagaa tgttggggtc
      841 ggcggccagc ccacgcgcca cccccacccg ctgctgctgg ccgcccgaga gctggtcggg
      901 gtagcgccgg cccatccgcg cttcgaggcc gaccagctcc atcagttcgg acgcccgtcg
      961 gtgcgcctcg tccttcggca cgccgttcag cagcggcacc gtggcgatgt tgtcgatcac
     1021 ctttcggtgg ggcagcaggc cggcgttctg catcacgtac ccgatgcgcc ggcgcagcgg
     1081 caccggcgga acctcggcca cgttctcgtc gtcgatccag acgatgccgc tgctcggatc
     1141 gaccattcgg ttgaccatgc gcagcagcgt ggtcttgccg cagccggacg agccgaccag
     1201 aaccgtcgtc tggccggtcg gagcctccag cgagaagccc tgaacggcct gcgttccgtc
     1261 cgggtaggac ttgctgacgt cctcgaagcg gatcatggcc cctactcaac agcatgacgc
     1321 cgacactggt aatacctagc cccgtttgag acgactattg tcgaggcagt tcaccccaac
     1381 gggcgtagac cgaacgaaag gcagcgatgg ccgagaccgt cagtcacaac cagcgcaggt
     1441 cgcgcttcga gatcgtggtc gatgacgagt tggcgggttt cgcgcactac gacctgtcca
     1501 acggtgtggc caccttcgac cacaccgagg tcaagccggc gttcaacggg cgcggcctgg
     1561 gcaccaccct ggtggccggc gccctcgatc aggtgcggca gcacggcatc tggaaggtgc
     1621 gcgccacctg ccccttcgtg gtgacctttc tgaggcggca ccccgaatac gccgacctcg
     1681 tggtgccctg agcccggctc ggatccgagg tcagtcccgg gcgtaccggg tgacgaagtt
     1741 gcgcagcagc cggtgggggt gctcggccac cccggacgtg cgcgccatct cgatcagaat
     1801 gtcgaagtcg tcggggtgga agtacccggc gttgcggtag atctgcatgc gctgcgcgag
     1861 tgagtcgatg tcgagctcgg ggtggaactg ggtgacgtac acgttgctgc cgtgccggta
     1921 catctgcacc gggcaggcga cgcccgtgcc cagcaccgtc acgccgggag gtgcgccgtt
     1981 gcaggcctcc ttgtggccga cgaaggcctg gaacgtggcc ggaatgcccg ccatcatcgg
     2041 atcgtcggac gccgcctcgg tcagtgtcac ctcgatggct ccgagcccct cgccgtaggt
     2101 gcgatcgacc gttccgccca gatgcacggt caccgtaccg atgccgtagc acatgcccag
     2161 aaagggaaaa tcggcatcaa cgatgtggtc caccacgttg cgaatgtcgc tctcgacccg
     2221 cagttgcagg tcggacttca ccgcgtccga gatgttgaac gggctgccgc cgagaaagac
     2281 cccggagtag tcctccaggt acagccgggg cagcggcccg gcctcgacgc gaaggtgcac
     2341 cagttcgtcc ggtgccaagc cggcgtgcac gcgcaccgac tcgtactcgt cgcgggcggc
     2401 cttgtcatgg tcacgggtgg cgagcagcac gaacggtttc acggcgagaa gggtagcggc
     2461 cagctcggcc gctccgcccc ggtctcggcc accagctgcc gcagcgccgc ggacggcccg
     2521 cccgcggccc gttgccgcag gtagctcggc ccgtgctcga gtagttcggc gatgaagccc
     2581 agctcgtcgg cacagtgcag ttcggcggcg atcgtttcca gccggttcag ccagtccatg
     2641 atgccctcgc gcatcggacg caccttctgc cccacccgcg gcgtcaccac atcggtgtcg
     2701 agcccgtagc gggcggcccg ccacttgttc tctttcatga accacatcgg cagccgggcg
     2761 atgccccgtc cgtcatcgag ctcgcgcgac atgtaggtgg ccaggcactg cgccagtgcg
     2821 gccatggcgc ccagttcggc gaaggtgggc accgagtcga gcacccggtt ctcgaccgtg
     2881 ccgaaggtgg acggacgcac atcccagcga atctcgctcg gcaccgtgat catgccggcc
     2941 tcttcgagtt cgctcgcgta gtgctcgaac tcgtcccagc tgtccagcgg atagggcagg
     3001 ccgttggtgg gcatctgttg aaacagcatg gtgcggtgcg aggcgtagcc ggtgtcttcg
     3061 ccctcccagt acggcgacga ggcggtcagg gcgatgaaat agggcgagaa ccgggccagg
     3121 ccgtaggtga tcggcagggc cttgtcgcgg tgatcgatgc cgacgtgcac atgggtgccg
     3181 aagatggaca tcagccggcc ccagtaggcg ttgcgttcgg ccaccacgtg atagcgcggg
     3241 gtgtcgaagt ggtgctggtc gtgggccggt gagcgcgggt gcacgcccgc gcccagcagc
     3301 agggtgtcgt cgccggcgat cgcgcgcacc cgctgcaggc tttcgcgcag gtcatccatc
     3361 gcctcgtcga cccgatgatg cacgccggtg acgagttcga tcatgttggt caggtattcc
     3421 tgccggatcg ggccgcgctt gggatcgtcc acggcgtcca gcaggcgctg agcccgcggc
     3481 accaactcga gcgagtcggc gtccaccagg gcgagctccc actcgacacc gagcgtcggg
     3541 ccgctggagg gcacgaagtc gactcgcatg cagatcccct gactggctgc tcgccgcggc
     3601 aaccgcaagg agtctagaac gcctcgagcg ccggcatcag gcctcgaccc acccgtagag
     3661 gcccttggtg gcgatggtgc gctcgaccac ccggttgtcg atgtcgccgc cgatcaccgt
     3721 cagcttgccg ttcgccagca cctcgaccac gacgtcgacc cgggtgctgg tcttggggct
     3781 ggtcgggccg gtgccgctca gcaccagatc gcccggcccg acaccggcgg tgccctcgtg
     3841 ggcgcggttg tgcttctgtc cccaggtgaa caggtcaccc gagaacgaga acttcggcac
     3901 cacgttgccg gccttgttcc agacccaggt ggcgaacaac gaactccagg gcatgcagcc
     3961 gccgtaggcg ttgcacttct tcttcttgcc ttcgacgccg cgctgacccc gggccacctt
     4021 gacgatcgcc tgctcgacat tcggcggccg gctggtgacc gaggcggtcg gcttgaccgc
     4081 gcccgcctgg cacaggctga gcgccgcctc gtccgcattc acgaacactg acgcgacgta
     4141 gccggtcttt ttcttgtagg tgaccttgtt ccacacgttc gacgcgtggg tgccgttgct
     4201 gaccgtgtcg ccgatggttc tgcacgtcat cgtgatcgcc gtcttgtgcg gtatgcgcag
     4261 cagcctcttg ctgctgatcg tgggctgctc gcgcagcgac aggggggtct tcttgttggt
     4321 ggcgaccgtg cccttgaccg ggggcagttg cgccttggcg gtggtggcgg acgcccgcgg
     4381 tgtggggctc tgctgcgtac cggacgcacc gtcggtgcac ccggtgaccg ccgcggcgac
     4441 caccagcgtc agtaccgcag tcgctgtacc ccgtgccgcc atgcgttcga cggtaacacg
     4501 ccgcccggac ggtgcgcgcg gcggtcgtcg ccgcgggcgc accgcccggt ctcccatggg
     4561 gtgggtggat ctgcggctca accgttgacg ccggccaggg tctcgccgta tgcggcatcg
     4621 gcgggcacgg cggacgaacc ggtgtcttcg agcagcagcg aataccaggt gtcggtgctg
     4681 cgggtgtagg tgaccgaccc ggcatcggcg atgctgccga cgtcttcgtg cggggtaccg
     4741 atcacgacgc cgtccgccat cgcttcgccg tcgtcgggat tgccgcccag cacgctgacc
     4801 gaccagccga agtgatcggc cgcctcgggt gcgccgggca caccgtccgc accctgacgc
     4861 agcgcccagg tgaggccgcc ggcccgattg gcccggaaca tggtgaccga accggcgccc
     4921 agcacgctgc cgatcgcctc gccgggggcc ccgacgatcg cggtcagctg gtcgagggtg
     4981 ttgatgccca gtgcgaccga cgacccgaag tagtcgccgc tctcgttggc gccgggaatg
     5041 ccggcggtgt tctggttgac ggcgcgcagc gacttcaccg tgcgggtggc cgggttgaag
     5101 ttgagcacgt gcacctggcc ggtgtacgtg gccgagccga tggtttcgcc cggcacaccg
     5161 accagcacat agtggtcgcg gtaggcgacc gcctcgccga accggtcacc gctctcggcc
     5221 gtgcctggca ctcctgccga gttctgggtg acgccgacgc cggcgaacgt ggtgggtgcg
     5281 gcgtccgtgg ccgacagcat cgtcacagag ccggcgttgc tgcgggtgcc gacggcctcg
     5341 ttgggcgtac cgatcacgat ggcccggccc tggccggcca gcgcggtgcc gaaccggtcg
     5401 ccgttctctg agctgccggg tacgcccggc g
//
LOCUS       DAOSUG010000161         5426 bp    DNA     linear   ENV 10-JUN-2024
DEFINITION  MAG TPA_asm: Micropruina sp. isolate AMR_MDS_2195
            NODE_6797_length_5426_cov_3.886241, whole genome shotgun sequence.
ACCESSION   DAOSUG010000161 DAOSUG010000000
VERSION     DAOSUG010000161.1
DBLINK      BioProject: PRJNA925477
            BioSample: SAMN32864262
            Sequence Read Archive: SRR4236673
KEYWORDS    WGS; ENV; Metagenome Assembled Genome; MAG; Third Party Data; TPA;
            TPA:assembly.
SOURCE      Micropruina sp. (wastewater metagenome)
  ORGANISM  Micropruina sp.
            Bacteria; Bacillati; Actinomycetota; Actinomycetes;
            Propionibacteriales; Nocardioidaceae; Micropruina.
REFERENCE   1  (bases 1 to 5426)
  AUTHORS   Abdulkadir,N., Saraiva,J.P., Zhang,J., Stolte,S., Gillor,O.,
            Harms,H. and Rocha,U.
  TITLE     Genome-centric analyses of 165 metagenomes show that mobile genetic
            elements are crucial for the transmission of antimicrobial
            resistance genes to pathogens in activated sludge and wastewater
  JOURNAL   Microbiol Spectr 12 (3), e0291823 (2024)
   PUBMED   38289113
REFERENCE   2  (bases 1 to 5426)
  AUTHORS   Abdulkadir,N.
  TITLE     Direct Submission
  JOURNAL   Submitted (24-MAR-2023) Environmental Microbiology, Helmholtz
            Center for Environmental Research -UFZ, Permoserstr 15, Leipzig
            04318, Germany
COMMENT     The annotation was added by the NCBI Prokaryotic Genome Annotation
            Pipeline (PGAP). Information about PGAP can be found here:
            https://www.ncbi.nlm.nih.gov/genome/annotation_prok/
            
            ##Genome-Assembly-Data-START##
            Assembly Method        :: SPAdes v. 3.11.1
            Genome Representation  :: Full
            Expected Final Version :: Yes
            Genome Coverage        :: Not Applicable
            Sequencing Technology  :: Illumina MiSeq
            ##Genome-Assembly-Data-END##
            
            ##Genome-Annotation-Data-START##
            Annotation Provider               :: NCBI
            Annotation Date                   :: 05/04/2023 04:57:10
            Annotation Pipeline               :: NCBI Prokaryotic Genome
                                                 Annotation Pipeline (PGAP)
            Annotation Method                 :: Best-placed reference protein
                                                 set; GeneMarkS-2+
            Annotation Software revision      :: 6.5
            Features Annotated                :: Gene; CDS; rRNA; tRNA; ncRNA
            Genes (total)                     :: 2,776
            CDSs (total)                      :: 2,750
            Genes (coding)                    :: 2,714
            CDSs (with protein)               :: 2,714
            Genes (RNA)                       :: 26
            tRNAs                             :: 24
            ncRNAs                            :: 2
            Pseudo Genes (total)              :: 36
            CDSs (without protein)            :: 36
            Pseudo Genes (ambiguous residues) :: 0 of 36
            Pseudo Genes (frameshifted)       :: 2 of 36
            Pseudo Genes (incomplete)         :: 32 of 36
            Pseudo Genes (internal stop)      :: 2 of 36
            ##Genome-Annotation-Data-END##
FEATURES             Location/Qualifiers
     source          1..5426
                     /organism="Micropruina sp."
                     /mol_type="genomic DNA"
                     /submitter_seqid="NODE_6797_length_5426_cov_3.886241"
                     /isolate="AMR_MDS_2195"
                     /isolation_source="Activated sludge of municipal
                     wastewater treatment plants"
                     /db_xref="taxon:2737536"
                     /environmental_sample
                     /geo_loc_name="Canada"
                     /lat_lon="49.25 N 123.1 W"
                     /collection_date="2015-06-16"
                     /metagenome_source="wastewater metagenome"
                     /note="metagenomic"
     gene            39..749
                     /gene="pyrH"
                     /locus_tag="PLF56_06565"
     CDS             39..749
                     /gene="pyrH"
                     /locus_tag="PLF56_06565"
                     /EC_number="2.7.4.22"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_013862400.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_function: GO:0033862 - UMP kinase activity [Evidence
                     IEA];
                     GO_process: GO:0015949 - nucleobase-containing small
                     molecule interconversion [Evidence IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="UMP kinase"
                     /protein_id="HOQ53261.1"
                     /translation="MTYHRIMLKLSGEMFGGSRGIGVDPDVVANLASQIKAVVDAGVQ
                     VAVVTGGGNFFRGAELSERGMDRDRADYMGMLGTVMNCLALQDFCEKAGVQTRVQSAI
                     AMGQVAEPYIPRRAERHLEKGRVVIFGAGSGMPYFSTDTVAAQRALEIGADVLLMGKQ
                     GTDGVYDSDPKTNPAALRFDELTYDDYLARDLKVADATAISLARDNSLPMIFFDLGVE
                     GNILRVVQGEKIGTTVHA"
     gene            779..1333
                     /gene="frr"
                     /locus_tag="PLF56_06570"
     CDS             779..1333
                     /gene="frr"
                     /locus_tag="PLF56_06570"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_002516897.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_component: GO:0005737 - cytoplasm [Evidence IEA];
                     GO_function: GO:0008135 - translation factor activity, RNA
                     binding [Evidence IEA];
                     GO_process: GO:0006412 - translation [Evidence IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="ribosome recycling factor"
                     /protein_id="HOQ53262.1"
                     /translation="MSDILSDAESKMKGAVEHAREEFAAIRTGRAHPAMFNKLMVDYY
                     GSPTPLQQLATFQVPEARTVLISPFDRASMAGVEKAIRDSDLGVNPANDGNVIRVVMP
                     QLSEERRREYVKLARTKAEEARVSVRNIRRHANDAVHKLVKDKDVGEDDARTAEKSLD
                     GSTKKYVDAIDELLKAKEAELLEV"
     gene            1333..2214
                     /locus_tag="PLF56_06575"
     CDS             1333..2214
                     /locus_tag="PLF56_06575"
                     /EC_number="2.7.7.41"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_013862402.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="phosphatidate cytidylyltransferase"
                     /protein_id="HOQ53263.1"
                     /translation="MAAEQQVPSEPAVRKSRAGRDLPAAIMVGVGLAATVVITLLFWN
                     WGFVLLVTAMLTLGAFELHDALKRIGMNSAVLPIMAGTVAVICGSYAAATQQGTNAVP
                     WPSVLLGFLGATVLTALIWRMPGGATGYVKDAAASLFTIGYIPLLGSFTSLILAAPNG
                     SAKMATILLVVMGTDTGGYAVGARIGKHPMAPQISPKKTWEGMGGSVLLAGAVAIPLT
                     IWWLNASWWIGLLLALCIVAAGTCGDLIESLIKRDVGIKDMSSFLPGHGGAMDRLDSI
                     LVAAPVAWLLLFLLIPN"
     gene            2225..3463
                     /gene="rlmN"
                     /locus_tag="PLF56_06580"
     CDS             2225..3463
                     /gene="rlmN"
                     /locus_tag="PLF56_06580"
                     /EC_number="2.1.1.192"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_008363289.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_function: GO:0008757 - S-adenosylmethionine-dependent
                     methyltransferase activity [Evidence IEA];
                     GO_function: GO:0016433 - rRNA (adenine) methyltransferase
                     activity [Evidence IEA];
                     GO_process: GO:0031167 - rRNA methylation [Evidence IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="23S rRNA (adenine(2503)-C(2))-methyltransferase
                     RlmN"
                     /protein_id="HOQ53264.1"
                     /translation="MTESPTPATDALRPDEISAYAALEDRVRAGASVPLALAPQRRGK
                     PPRHWLDSSTDERQAAVQQLGLPKFRADQISRHVFEGLDLDPSGWTDLPAAARAQVAE
                     SFFPRLLTDVRPLSCDGGTTLKTLWRLHDGSLIESVLMRYGVPGIRSSGSGRNRATLC
                     ISSQAGCGMACPFCATGQGGLQRNLSAAEILAQVLAGARALHDGTIPGGPGRINNIVF
                     MGMGEPMANYKAIMAAIRTMTAPGPHGLGISARGITVSTVGLVPRIAQLAGEGLAVTL
                     AVSLHAPDDELRDELCPINTRWNVRELVDAAHAYFVATKRRVSIEYALIRDVNDQPER
                     AALLADVLRRDGGHGWVHVNLIPLNPTPGSKWTASRRADEKAFVAALERRGVAVTVRD
                     TRGREIDGACGQLAATAAAG"
     gene            3486..5054
                     /locus_tag="PLF56_06585"
     CDS             3486..5054
                     /locus_tag="PLF56_06585"
                     /EC_number="1.2.1.79"
                     /inference="COORDINATES: protein motif:HMM:NF006916.0"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="succinic semialdehyde dehydrogenase"
                     /protein_id="HOQ53265.1"
                     /translation="MRSLVERQHHVLDDAQLADLTSLIFAEAPTEHRLVHTPLTGLPL
                     AEIPLSTRSDVGCAVQQARSALRAWTALSHRRRAAVVLDFHDRLLAQREDVLDLIQLE
                     TGKTRANAFEEVAEVANVARYYARSAAKLLAPRRFRGALPVLGAATERRLPRGVVGII
                     TPWNYPFALSLGDALAALLAGNAVVLRPDPRTCLSVLWGLRLLLAAGLPPGVLQVLTG
                     EGDVIGAAVARGADYVCFTGSTGTGRQVARLAADRLVGCSLELGGKNALYVRADAPLE
                     LAVDVALRSAFAAGGQVCAHTERLVVHDDIADAFVPRFLEAVQGMRLGVALDYGYDMG
                     SLFGPDQLQRVGDHVRDAVRHGARVLTGGRARPDLGPWVYEPTVLDGVTAAMDVRDAE
                     TFGPVVSIYRVRDDEAAIRLINDSDYGLHATIVSADRAAARRLAPRLRTGTVSINESF
                     IASWGATGAAMGARGHSGSGGRHGAEGLLKYTVVQSVAEQRVGSLAVDRTAQRRFAGL
                     FSGGLIVLRRSGLR"
     gene            5102..>5426
                     /locus_tag="PLF56_06590"
     CDS             5102..>5426
                     /locus_tag="PLF56_06590"
                     /inference="COORDINATES: protein motif:HMM:NF021523.2"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="DUF2252 family protein"
                     /protein_id="HOQ53266.1"
                     /translation="MPRHWLVQTRLIGQRVVEYQQALQTVSDPLLGWTEVDGRQYYVR
                     QFRNMKGTVPLDALTATALADYAGIVGHLLAKGHARTSGASMIAGYLGKGEKASKAFS
                     RFARLY"
BASE COUNT          813 a         1891 c         1908 g          814 t
ORIGIN      
        1 cagccccgag cgatcaccac ccccgagcga ggagaacaat gacctaccac cgcatcatgc
       61 tcaagctgtc cggcgagatg ttcggcggca gccgcggcat cggcgtcgac ccggacgtgg
      121 tggccaacct cgccagccag atcaaggcgg tggtcgacgc cggcgtgcag gtcgcggtgg
      181 tgaccggcgg cggcaacttc ttccgcggcg cggagctctc cgagcgcggc atggaccgcg
      241 accgggccga ctacatgggc atgctcggca ccgtgatgaa ctgcctggcc ctgcaggact
      301 tctgcgagaa ggccggggtg cagacccgcg tgcagtcggc gatcgccatg ggccaggtcg
      361 ccgagcccta cattccgcgc cgcgccgagc ggcacctcga aaagggacgg gtggtgatct
      421 tcggcgcggg cagcggcatg ccgtacttca gcaccgacac cgtcgccgcc cagcgggctc
      481 tcgaaatcgg cgcggacgtg ctgttgatgg gcaagcaggg caccgacggc gtctacgact
      541 ccgaccccaa gacgaacccg gcggccctcc gcttcgacga actgacctac gacgactacc
      601 tggcccgtga cctgaaggtg gccgatgcca ccgcgatcag cctggcccgc gacaactctt
      661 tgccgatgat cttcttcgac ctcggtgtcg agggcaacat cctgcgcgtc gtgcagggtg
      721 agaagatcgg taccaccgtc cacgcctgag cagcgaacca ccgaaaggaa ctcggatgat
      781 ctccgacatt ctgtccgacg ccgaaagcaa gatgaagggc gcggtcgagc atgctcgcga
      841 agagttcgcc gcgatccgca ccggccgcgc gcaccccgcc atgttcaaca agctgatggt
      901 cgactactac gggtcgccca ccccgttgca gcagctggcc acctttcagg tgcccgaggc
      961 ccgcaccgtg ctgatcagcc ccttcgatcg agcgtcgatg gcgggcgtcg agaaggcgat
     1021 ccgggactcc gacctgggcg tcaacccggc caacgacggc aacgtgatcc gggttgtgat
     1081 gccccagctg tccgaagagc ggcgccgcga atacgtcaag ctggcgcgca ccaaggccga
     1141 agaggcacgg gtgtcggtgc gcaacattcg ccggcacgcc aacgacgccg tccacaagct
     1201 ggtcaaggac aaggacgtcg gcgaggacga cgcgcgcacg gccgagaaga gcctggacgg
     1261 gtcgaccaag aagtacgtcg acgcgatcga cgagctgctc aaggccaagg aagccgagct
     1321 gctcgaggtc tgatggcagc agagcagcag gtgccctccg aaccggccgt ccgaaagtcg
     1381 cgcgccggac gggatctgcc tgccgcgatc atggtcggtg tcggcctggc cgccaccgtg
     1441 gtgatcaccc tgctgttctg gaactggggg ttcgtgctgc tggtcaccgc catgctcacc
     1501 ctcggcgcgt tcgagttgca cgatgcgctc aagcgcatcg gcatgaactc ggcggtgctt
     1561 cccatcatgg ccggcaccgt cgccgtgatc tgcggctcgt acgcggcggc cacccaacag
     1621 ggcaccaacg ccgtcccctg gccctcggtg ctgctgggtt ttctgggcgc cacggtgctc
     1681 accgcgctga tctggcgcat gcccggtggc gccaccggct acgtcaagga cgccgccgcc
     1741 agcctgttca cgatcggcta cattccgctg ctcggctcgt tcaccagcct gattctggcc
     1801 gccccgaacg gttcggccaa gatggccacg atcctgctgg tcgtgatggg caccgacacc
     1861 ggcggctacg cggtgggcgc ccgcatcggc aagcacccga tggcccccca gatcagcccc
     1921 aagaagacct gggagggcat gggtggttcg gtgctgctgg ccggcgcggt cgcgataccg
     1981 ctgaccatct ggtggctgaa cgcctcgtgg tggatcggtc tgctgctggc cctgtgcatc
     2041 gtggcggccg gcacctgcgg cgacctgatc gagtcgctga tcaaacgtga cgtgggcatc
     2101 aaggacatgt cgtcgttcct gcccggccac ggcggtgcca tggaccggct cgactcgatc
     2161 ctggtcgccg ccccggtggc ctggctgctg ctcttcctgc tgatcccgaa ctgagttgat
     2221 gtcgatgact gagtcaccaa cgccggccac ggacgcgctg cgtcccgacg agatcagcgc
     2281 ctacgcggca ctggaggacc gggtacgcgc aggcgcgagc gtgccgctcg ccctggcgcc
     2341 gcaacgccgg ggcaagccac cccggcactg gctcgacagc agcacggacg agcggcaggc
     2401 ggccgtccag cagctggggc tgccgaagtt ccgcgccgac cagatctcgc ggcacgtgtt
     2461 cgaggggctc gacctcgacc cgtccggctg gacggacctg cccgcggcgg cgcgcgccca
     2521 ggtggccgag agcttctttc cgcggctgct gaccgacgtg cgtccgctca gctgcgacgg
     2581 cggcaccacg ctgaagacct tgtggcggct gcatgacggg tcgctgatcg agtcggtgct
     2641 gatgcgctac ggggtgcccg gcatccgttc gtccggctcg ggacgcaacc gggccacgct
     2701 ctgcatcagc tcgcaggcgg gctgcggcat ggcctgcccg ttctgcgcca ccgggcaggg
     2761 cggcctgcag cgcaacctga gcgccgccga gattctggcg caggtgctgg ccggcgcccg
     2821 cgccctgcac gacggcacga ttcccggtgg cccgggccgg atcaacaaca tcgtgttcat
     2881 gggcatgggc gagccgatgg ccaactacaa ggcaatcatg gcggcgatcc gcaccatgac
     2941 cgcacccggt ccgcacggcc tgggcatcag cgcccggggc atcaccgtct cgacggtcgg
     3001 tctggtgccc cgcatcgccc aactggccgg cgaagggctg gcggtcacac tggcggtcag
     3061 cctgcacgcc cccgatgacg aactgcgcga cgaactgtgc ccgatcaaca cccgctggaa
     3121 cgtgcgtgaa ctcgtggacg cggcccacgc ctacttcgtg gccaccaagc gacgggtgtc
     3181 gatcgagtac gcgctgatcc gcgacgtgaa cgaccagccg gagcgggccg cgctgctggc
     3241 ggacgtgctg cgccgcgacg gcggacacgg ctgggtgcac gtcaacctga ttccgctcaa
     3301 cccgaccccg ggctcgaaat ggacggcctc gcgacgtgcc gacgagaagg cgttcgtcgc
     3361 cgctctggag cgtcgcgggg tcgcggtcac ggtgcgcgac actcgcggcc gcgagatcga
     3421 cggagcctgc gggcagcttg ccgcaacggc tgccgcgggg tgacccggcg cgagtagggt
     3481 gaaccatgcg ttcgttggtc gagaggcagc atcacgttct cgacgacgcc caactggccg
     3541 acctgaccag cctgatcttc gctgaggcac cgaccgagca tcgcctcgtc cacaccccgc
     3601 tgacggggct tccgctcgcc gagatcccgc tgtccactcg ctcggacgtg gggtgcgcgg
     3661 tgcagcaggc acgctcggcg ctgcgggcct ggaccgcgct gagccaccgt cgccgggccg
     3721 ccgtggtgct cgactttcac gaccgcctgc tggcccagcg tgaggacgtc ctcgacctga
     3781 ttcagctcga gaccggcaag acccgtgcca acgcgttcga agaggtcgcc gaggtcgcca
     3841 acgtggcgcg ctactacgcg cggtccgcgg cgaagctgct ggcaccgcgt cgattccggg
     3901 gcgcgttgcc ggtgctgggt gccgccaccg agcggcggct gccgcggggc gtggtcggca
     3961 tcatcacgcc ctggaactac ccgttcgcgt tgtcgctcgg cgacgcgctg gccgcgctgc
     4021 tggccggcaa cgcggtggtg ctgcggcccg atccgcgcac ctgcctgtcg gtgctgtggg
     4081 ggttgcgcct gctgctggcc gcgggcctgc caccgggggt gttgcaggtg ctgaccggcg
     4141 agggcgacgt catcggcgcc gctgtggccc ggggcgccga ctacgtgtgc ttcaccggct
     4201 cgaccggcac cggacgtcag gtggcccggc tggccgccga ccggctggtc ggctgctcgc
     4261 tcgaactcgg cggcaagaac gccctgtacg tgcgagccga cgccccgctc gagctggccg
     4321 tcgacgtggc cctgcgcagt gcgttcgccg ccggcggcca ggtgtgtgcc cacaccgagc
     4381 ggttggtggt gcacgacgac atcgcggacg cgttcgtgcc ccggttcctc gaggccgtgc
     4441 agggcatgcg cctcggggtg gcactggact acggctacga catgggttcg ctgttcggcc
     4501 ccgaccagct gcaacgcgtc ggcgaccacg tgcgtgacgc cgtgcgccac ggcgcccggg
     4561 tgctgaccgg gggacgggcc cgtccagacc tcggcccgtg ggtgtacgag ccgacggtgc
     4621 tcgacggggt gaccgccgcg atggacgtgc gcgacgccga gaccttcggc ccggtggtca
     4681 gcatctaccg ggtgcgcgac gacgaggcgg cgatccggct gatcaacgac tccgactacg
     4741 gtctgcacgc cacgatcgtc agcgccgacc gtgcggcggc acggcggctc gcaccccggc
     4801 tgcgcaccgg cacggtcagc atcaacgagt ccttcatcgc ctcgtggggg gccaccgggg
     4861 cggccatggg tgcgcgcggg cactcgggca gcggcgggcg gcacggtgcc gaggggctgc
     4921 tcaagtacac ggtggtgcag agcgtggccg agcagcgggt cggctcactg gccgtcgacc
     4981 ggaccgcaca acgacgcttc gccggcctgt tcagcggggg tctgatcgtg ctgcggcgtt
     5041 ccggcctgcg ctgatcgggc gctggtcgag tggtgtgtcc ctcaaccggt acgtgcttcg
     5101 ggtgccacgc cactggcttg tccagacccg cttgataggc cagcgggtgg tggagtacca
     5161 gcaggcgttg cagaccgtct cggatcccct gctcggctgg acggaggtcg acggccggca
     5221 gtactacgtg cggcagttcc gcaacatgaa gggcaccgtg ccgctggacg ccctgaccgc
     5281 caccgcgctg gccgactacg ccggcatcgt cgggcacctg ctggccaagg ggcatgcgcg
     5341 cacgtccggg gcgtccatga tcgcggggta cctgggcaag ggcgagaagg ccagcaaggc
     5401 gttctcgcgg tttgcccggc tctacg
//
LOCUS       DAOSUG010000162         5415 bp    DNA     linear   ENV 10-JUN-2024
DEFINITION  MAG TPA_asm: Micropruina sp. isolate AMR_MDS_2195
            NODE_6814_length_5415_cov_4.916045, whole genome shotgun sequence.
ACCESSION   DAOSUG010000162 DAOSUG010000000
VERSION     DAOSUG010000162.1
DBLINK      BioProject: PRJNA925477
            BioSample: SAMN32864262
            Sequence Read Archive: SRR4236673
KEYWORDS    WGS; ENV; Metagenome Assembled Genome; MAG; Third Party Data; TPA;
            TPA:assembly.
SOURCE      Micropruina sp. (wastewater metagenome)
  ORGANISM  Micropruina sp.
            Bacteria; Bacillati; Actinomycetota; Actinomycetes;
            Propionibacteriales; Nocardioidaceae; Micropruina.
REFERENCE   1  (bases 1 to 5415)
  AUTHORS   Abdulkadir,N., Saraiva,J.P., Zhang,J., Stolte,S., Gillor,O.,
            Harms,H. and Rocha,U.
  TITLE     Genome-centric analyses of 165 metagenomes show that mobile genetic
            elements are crucial for the transmission of antimicrobial
            resistance genes to pathogens in activated sludge and wastewater
  JOURNAL   Microbiol Spectr 12 (3), e0291823 (2024)
   PUBMED   38289113
REFERENCE   2  (bases 1 to 5415)
  AUTHORS   Abdulkadir,N.
  TITLE     Direct Submission
  JOURNAL   Submitted (24-MAR-2023) Environmental Microbiology, Helmholtz
            Center for Environmental Research -UFZ, Permoserstr 15, Leipzig
            04318, Germany
COMMENT     The annotation was added by the NCBI Prokaryotic Genome Annotation
            Pipeline (PGAP). Information about PGAP can be found here:
            https://www.ncbi.nlm.nih.gov/genome/annotation_prok/
            
            ##Genome-Assembly-Data-START##
            Assembly Method        :: SPAdes v. 3.11.1
            Genome Representation  :: Full
            Expected Final Version :: Yes
            Genome Coverage        :: Not Applicable
            Sequencing Technology  :: Illumina MiSeq
            ##Genome-Assembly-Data-END##
            
            ##Genome-Annotation-Data-START##
            Annotation Provider               :: NCBI
            Annotation Date                   :: 05/04/2023 04:57:10
            Annotation Pipeline               :: NCBI Prokaryotic Genome
                                                 Annotation Pipeline (PGAP)
            Annotation Method                 :: Best-placed reference protein
                                                 set; GeneMarkS-2+
            Annotation Software revision      :: 6.5
            Features Annotated                :: Gene; CDS; rRNA; tRNA; ncRNA
            Genes (total)                     :: 2,776
            CDSs (total)                      :: 2,750
            Genes (coding)                    :: 2,714
            CDSs (with protein)               :: 2,714
            Genes (RNA)                       :: 26
            tRNAs                             :: 24
            ncRNAs                            :: 2
            Pseudo Genes (total)              :: 36
            CDSs (without protein)            :: 36
            Pseudo Genes (ambiguous residues) :: 0 of 36
            Pseudo Genes (frameshifted)       :: 2 of 36
            Pseudo Genes (incomplete)         :: 32 of 36
            Pseudo Genes (internal stop)      :: 2 of 36
            ##Genome-Annotation-Data-END##
FEATURES             Location/Qualifiers
     source          1..5415
                     /organism="Micropruina sp."
                     /mol_type="genomic DNA"
                     /submitter_seqid="NODE_6814_length_5415_cov_4.916045"
                     /isolate="AMR_MDS_2195"
                     /isolation_source="Activated sludge of municipal
                     wastewater treatment plants"
                     /db_xref="taxon:2737536"
                     /environmental_sample
                     /geo_loc_name="Canada"
                     /lat_lon="49.25 N 123.1 W"
                     /collection_date="2015-06-16"
                     /metagenome_source="wastewater metagenome"
                     /note="metagenomic"
     gene            complement(<1..2193)
                     /locus_tag="PLF56_06595"
     CDS             complement(<1..2193)
                     /locus_tag="PLF56_06595"
                     /inference="COORDINATES: ab initio
                     prediction:GeneMarkS-2+"
                     /note="Derived by automated computational analysis using
                     gene prediction method: GeneMarkS-2+."
                     /codon_start=1
                     /transl_table=11
                     /product="hypothetical protein"
                     /protein_id="HOQ53267.1"
                     /translation="MSMLLRDVIDIPEKAGAEDYVLRLTDSVEESHLARTLDDYVVTT
                     ALAESFDGALSLVADAVRQGVSRGAFLTGSFGSGKSHFMAVLYALLRQVPQARGLDLL
                     GPVVARHDPVLQGKKVLPLAFHFLAAESMEQALFSGYLNQIRALHPGCSLPALHRSDQ
                     ILADADHLRDLHGDVAFFDGLNAGAAAGGDDVWGAFLGASTWTPERYEAARAAAPGSQ
                     LRQELVSALAGTYFTSYTAQAEFVDLDSGLAAISQHAKGLGYDAVVLFLDEVVLWLAF
                     AVRDASWFARESQKLSKLVEGGLGARAIPLVSFVARQLDLRRWFADAGASGAQQEALD
                     QAFRHQEGRFAQLRLGDDNLAYVANRRLLRPKNAEAARVLDDAFRRIDRSPGVWDVLL
                     DGVNSDDQHRGADEAAFRLTYPFSPALVSTLRSLASVMQRERTALKVMQQMLVDRRDT
                     LTVEDVIPVGDAFDYIVTGQSGQALDAQVAALFRSAEKLYADKLRPLLLKPYPVTEKE
                     LAAGAQRPAGLAADERLAKTLLLSAVAPHVPALKGLDAARLASLNHGSIVAMLPGGEV
                     TMVAAKVSTWSKSVPEIKADSASANPTIRVVLSDVDYESVVERARGEDNEGRRRELIK
                     ALVVESLALALDPPDMLGAQRHRFVWRGSGREVYLVFGNVRDANWLTDDHFRAPAGAW
                     KFVIDVPFDDEGHSSVEDVERVDRLIASNLQERTLVWIPRFFSSERMRD"
     gene            complement(2190..>5415)
                     /gene="pglX"
                     /locus_tag="PLF56_06600"
     CDS             complement(2190..>5415)
                     /gene="pglX"
                     /locus_tag="PLF56_06600"
                     /EC_number="2.1.1.72"
                     /inference="COORDINATES: protein motif:HMM:NF033451.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=2
                     /transl_table=11
                     /product="BREX-2 system adenine-specific
                     DNA-methyltransferase PglX"
                     /protein_id="HOQ53268.1"
                     /translation="EWLLQAIEHLRRTPATAALVDARSALWTVSPSGRAATALLAVWR
                     ERTEDGELVRDFADPALDTRFLGDLYQDLSEHAKKTYALLQTPDFVEEFILDRTLEPA
                     LAERPLEGFKLIDPACGSGHFLLGAFARFLGRWQRHAPGLEPRLRVQKALDAVWGVDI
                     NPFAIAIARFRLLLAALHAVGATSLEGAPGFTIHVLAGDSLYFSHDQQYLPALGDDFA
                     YAGEDKGSLLAALNHGQYDAVVANPPYITVKDRKLNAAYRERYSHARGKYALSVPFME
                     LLFSLAKTCQGDRPAGWVGQITSNSFMKREFGVSVIEKFLPGKDLVEIIDSEGAWIPG
                     HNMDGTPTSIIVGRQQAPVSPVIRTVLSKGLRESRPVGVGAGPYWRSIVEHILEPGYD
                     DPWISVVDLGRDFFARHPWTLSGGGALEVIEKIERSSARLSDLASSVGITSFTLEDDV
                     YVRDRESFATRRVPSQYLRPMVLGDGVRDWSLTEYPMTIFPYDDDFRPISVSRSSELM
                     RCMWPWRVNLAKSVMFNGSTKVASGLLWTEFGRLTVPKLRVPRSIVFAEVATHNHFVI
                     DDGGKVFKQTAPVIKLDKSATGETHSRVAGVLNSSVACFWLKQMCKPKGGAADVAWLR
                     TYQFNGTTVKDLPLPRSMPVEPAKVLDRLARELSTISVSLMLDSGIVSAERLRVAEAE
                     QLSIRRRMIAQQEELDWTVYRSYGLIENELVYPGEPPEVSLGERAFEIFLARTVAAGE
                     EETSWFSRHGSTPITNIPTHWPQDYRELVQRRIDAIESVDAIRLLEVPEYKRRWAFEP
                     FATQIEKALRSWLLDRLEDRSLWFSRQGHPTPRSVAQLADELGRDAEFVSVVELWRGK
                     GVRLVDALAVLLEPEAVPYLAAHRLKDPGLRKFVDWQRTWELQRQEDAMRPAEDAPEA
                     EKKAANARADMFAATITVPPKYTSTDFRKPSYWSARGKLDVPKERFIVYPGAGRATDP
                     TALLGWAGWDHAQQSLALATIIAAREADGAPDEVLVPLVAGMAELQPWVQQWHAEVDP
                     AFGQSPAEFTGQELARRARQVGRSLEQLAAWRPPAATRGRRAKA"
BASE COUNT          981 a         1870 c         1676 g          888 t
ORIGIN      
        1 gtcccgcatg cgctcgctcg agaagaaccg cgggatccag accagcgtcc gctcctgcag
       61 gttggacgcg atgagtcggt cgacgcgttc cacgtcttcc acggacgagt ggccctcgtc
      121 gtcgaacggc acgtcgatca cgaacttcca ggcgccggcc ggcgcgcgga agtggtcgtc
      181 ggtcagccag ttcgcgtcgc ggacgtttcc gaagaccaag tagacctcgc gaccggaacc
      241 acgccacacg aaacggtgtc gctgcgcccc caacatgtcg ggcggatcga gggccaacgc
      301 gaggctctcg acgaccagag ccttgatcag ttcgcggcgg cgtccctcgt tgtcctcacc
      361 gcgggcgcgc tcaacgaccg actcgtagtc gacgtccgac agcacgaccc tgatagttgg
      421 attcgcagag gccgagtcgg ccttgatctc cggcactgac ttcgaccacg tgctcacctt
      481 ggccgcgacc atcgtcacct cgcccccggg cagcatggcc acgatcgacc cgtggttcag
      541 cgacgcgagc cgggcggcgt ccagtccctt gagcgcgggg acgtgcggcg ctaccgccga
      601 cagcagcagc gtcttggcca gccgttcgtc cgctgcgagg ccggccggac gctgggcgcc
      661 cgccgccagc tccttctcgg tcaccggata gggcttcagc agcagtggcc ggagcttgtc
      721 ggcgtacagc ttctccgccg accggaagag ggcggccacc tgggcgtcca gcgcctgtcc
      781 gctctgtccg gtgacgatgt agtcgaaggc gtcccccacg gggatcacgt cctcgacggt
      841 cagcgtgtcg cgccggtcga ccagcatttg ttgcatgacc ttgagcgcgg tccgctcccg
      901 ctgcatgacc gacgccagcg accgcagcgt cgacaccagg gcaggcgaga acgggtacgt
      961 cagccggaac gccgcctcgt cggccccccg gtgctggtcg tcggagttga cgccgtcgag
     1021 caggacgtcc cagacgccgg ggctgcggtc gatccgccgg aacgcgtcgt cgagcacgcg
     1081 cgccgcctcg gcgttcttgg gacgcagcag gcgtcggttg gcgacgtagg cgaggttgtc
     1141 gtcaccgagt cggagctgcg cgaaccggcc ctcctggtgc cggaacgcct ggtccagggc
     1201 ttcctgctgc gcgccggacg cccccgcgtc cgcgaaccag cgccgcaggt cgagctgccg
     1261 cgccacgaac gacaccaacg ggatcgcccg cgcccccaat ccgccttcga ccagcttcga
     1321 cagcttctgg ctttcccgag cgaaccagga ggcatcgcgc accgcgaagg ccaaccagag
     1381 cacgacctcg tcgaggaaca gcacgacggc gtcgtaccca agccccttgg catgctggga
     1441 gatcgccgcc agcccggagt cgaggtcgac gaactcggcc tgcgccgtgt acgaggtgaa
     1501 gtacgtgccg gccagcgcac tcaccagttc ctggcgcagc tgcgagcccg gcgctgcggc
     1561 ccgcgccgcc tcgtaacgtt caggagtcca ggtactggcg cccaggaagg cgccccagac
     1621 gtcgtcgccc ccagcggcgg cgcccgcgtt cagcccgtcg aagaacgcca cgtcgccgtg
     1681 gaggtcgcgg aggtgatcgg cgtcggcgag gatctggtcg gaacggtgca gcgccggcag
     1741 tgagcagccg gggtgcaggg cgcggatctg gttcaggtag cccgagaaca gcgcttgctc
     1801 catggactcg gcagccagga agtggaacgc caggggcagt actttcttgc cctgaagcac
     1861 cggatcgtgc cgcgccacga ccgggcccag caggtcaagc ccccgcgcct gtgggacctg
     1921 ccgcagcagc gcatacaaga ccgccatgaa gtggctcttg cccgacccga acgatcccgt
     1981 gaggaaggct ccgcggctca cgccttggcg cacggcgtct gccaccaggc tgagcgcccc
     2041 gtcgaacgac tcggccagcg cggtcgtcac cacgtagtcg tcgagcgtcc gcgcgaggtg
     2101 ggattcctcg acgctgtcgg tgagccgcaa tacatagtcc tcggcgccgg ccttctcggg
     2161 gatgtcgatg acatccctca acagcatgct catgccttcg cccttctccc ccgagtggca
     2221 gccggcggac gccaggccgc caactgctcc aacgatcggc cgacctgcct ggcccgacgt
     2281 gccagctcct gcccggtgaa ctcggccggg ctctgcccga acgcggggtc gacctcggcg
     2341 tgccactgct ggacccatgg ctgcagctcg gccatgcctg cgaccagcgg gaccagcacc
     2401 tcgtcgggcg ccccgtcggc ctcgcgtgcg gcgatgatgg tggccagcgc cagggactgt
     2461 tgcgcgtggt cccagcccgc ccaccccagc agcgccgtcg gatcggtggc ccggcccgcg
     2521 cccgggtaca cgatgaagcg ctccttcggc acgtcaagct tcccgcgcgc cgaccagtac
     2581 gacggcttcc gaaagtccgt ggacgtgtac ttcgggggca cagtgatcgt cgccgcgaac
     2641 atgtcggcgc gcgcgttcgc ggccttcttc tcggcctcgg gcgcgtcctc tgctggacgc
     2701 atggcgtcct cctgtcgctg caactcccac gtgcgctgcc agtcgacgaa cttccgcagc
     2761 ccggggtcct tcaaccggtg cgccgccagg tacgggacgg cctccggctc cagcaagacg
     2821 gccagcgcgt cgaccagtcg aacgcccttg ccccgccaca gctctaccac cgacacgaac
     2881 tcggcgtctc ggcccagctc gtcagccagt tgcgccaccg agcgaggcgt cggatgcccc
     2941 tgccgcgaga accacaacga ccgatcctca agtcggtcga gcagccagct acgcagtgcc
     3001 ttctcgatct gcgtcgcaaa cggctcgaaa gcccaccgcc gtttgtactc cggcacctcc
     3061 agcagccgga tcgcgtccac cgactcgatc gcgtcgatcc gacgctgcac cagctcgcgg
     3121 tagtcctgcg gccagtgtgt ggggatgttc gtgatcggcg tagacccgtg ccgagagaac
     3181 caagaggtct cctcctcacc ggcggccact gtgcgcgcga gaaagatctc gaacgctcgc
     3241 tctcccagac tcacttccgg tggttctcca gggtagacca attcattttc aatcaggcca
     3301 tacgaccggt acaccgtcca gtccagctct tcctgctgcg caatcatgcg ccgtcggatt
     3361 gacaactgct ccgcctccgc gacacggagc cgctcagcag aaactatacc tgaatcaagc
     3421 atcagcgata cagaaatcgt cgacaattcg cgagctagtc tgtccagcac cttcgccggc
     3481 tccaccggca tggacctagg tagcggcaga tctttcactg tagtcccgtt gaattgatac
     3541 gttcgcaacc aagccacatc ggctgcgccg cccttaggct tgcacatttg ctttaaccag
     3601 aagcaagcaa ctgatgagtt gagaacgccc gcgacccgag agtgcgtttc gcccgtggcg
     3661 ctcttgtcca gcttgattac gggagcagtc tgcttgaaca ccttccctcc gtcatcaatg
     3721 acgaagtggt tatgagtggc cacctcagca aacacgatag atctgggcac gcgcaacttc
     3781 ggcacggtca gccgcccgaa ctcggtccac aacaatcccg acgcgacctt cgttgatccg
     3841 ttgaacatga cgctcttcgc aagattcact cgccacggcc acatacagcg catcagctcg
     3901 cttgatcgtg acaccgaaat gggcctgaaa tcatcgtcat acgggaatat cgtcatcggg
     3961 tattcagtca gagaccagtc gcgaacaccg tcccccaaga ccattggtcg gaggtactgc
     4021 gacggcaccc tgcgagtcgc gaatgattcc cgatcacgga catacacatc gtcttcgagc
     4081 gtgaagctcg tgataccaac tgagctagct aggtcggaaa gcctagcgga agaccgctca
     4141 atcttctcga tcacttctag agccccgccc ccggagagcg tccaaggatg ccgcgcaaag
     4201 aaatcacgcc ccaggtcgac cacagaaatc catgggtcat catatcctgg ctccagaatg
     4261 tgctcgacga tggatcgcca gtatggcccg gcaccaacac ctaccgggcg gctttcacgc
     4321 aaccccttac tcagaacggt cctaatcacc ggcgacaccg gcgcctgctg gcgtcccaca
     4381 ataatcgatg tcggagtgcc atccatgtta tgacccggaa tccaagcccc ttccgagtcg
     4441 atgatttcaa cgagatcctt tcctggcaag aacttctcga tgactgaaac cccaaactcc
     4501 ctcttcatga aggaattcga cgtaatctgt ccaacccaac cagcaggcct atcgccctga
     4561 cacgtcttcg cgaggctgaa cagcagctcc atgaacggga ccgatagggc atatttccct
     4621 ctcgcatggc tgtatcgctc ccggtaggcg gcgttgagtt tccggtcctt taccgtgatg
     4681 tacggaggat tagcaacaac cgcatcgtat tgcccgtggt tcagggcagc aaggagactg
     4741 cccttatcct cgcccgcata tgcgaaatcg tcacccaaag ccggcaagta ctgctggtcg
     4801 tggctgaagt acagcgaatc accggcgagc acatggatcg tgaatcctgg cgccccttcg
     4861 agcgaagtcg ctccgacggc gtgcagggca gccagcagca gccgaaaccg cgcgatggcg
     4921 atggcaaagg ggttgatgtc gacgccccag acggcgtcca aggccttctg cacgcggagg
     4981 cgcggctcga ggccgggggc gtgtcgctgc cagcggccga ggaagcgggc gaaggcgccg
     5041 agcaggaagt gccccgaacc gcaggcaggg tcgatcaact tgaagccctc cagcggccgc
     5101 tcggccaagg caggctcgag ggtgcggtcg aggatgaact cctcaacgaa gtcgggggtc
     5161 tgcagcaggg cgtaggtctt cttggcgtgc tcggagaggt cctggtacag gtcgccgagg
     5221 aaccgggtgt cgagggccgg gtcggcgaag tcccgcacga gttcgccatc ttcagttcgc
     5281 tcgcgccaga cggcgagtag ggcggtggcg gcgcgcccgg acggcgagac ggtccacaag
     5341 gccgagcgcg cgtcaacgag cgcggcggtg gcgggcgtcc gacggaggtg ctcgattgct
     5401 tggagaagcc actcg
//
LOCUS       DAOSUG010000163         5400 bp    DNA     linear   ENV 10-JUN-2024
DEFINITION  MAG TPA_asm: Micropruina sp. isolate AMR_MDS_2195
            NODE_6830_length_5400_cov_4.840412, whole genome shotgun sequence.
ACCESSION   DAOSUG010000163 DAOSUG010000000
VERSION     DAOSUG010000163.1
DBLINK      BioProject: PRJNA925477
            BioSample: SAMN32864262
            Sequence Read Archive: SRR4236673
KEYWORDS    WGS; ENV; Metagenome Assembled Genome; MAG; Third Party Data; TPA;
            TPA:assembly.
SOURCE      Micropruina sp. (wastewater metagenome)
  ORGANISM  Micropruina sp.
            Bacteria; Bacillati; Actinomycetota; Actinomycetes;
            Propionibacteriales; Nocardioidaceae; Micropruina.
REFERENCE   1  (bases 1 to 5400)
  AUTHORS   Abdulkadir,N., Saraiva,J.P., Zhang,J., Stolte,S., Gillor,O.,
            Harms,H. and Rocha,U.
  TITLE     Genome-centric analyses of 165 metagenomes show that mobile genetic
            elements are crucial for the transmission of antimicrobial
            resistance genes to pathogens in activated sludge and wastewater
  JOURNAL   Microbiol Spectr 12 (3), e0291823 (2024)
   PUBMED   38289113
REFERENCE   2  (bases 1 to 5400)
  AUTHORS   Abdulkadir,N.
  TITLE     Direct Submission
  JOURNAL   Submitted (24-MAR-2023) Environmental Microbiology, Helmholtz
            Center for Environmental Research -UFZ, Permoserstr 15, Leipzig
            04318, Germany
COMMENT     The annotation was added by the NCBI Prokaryotic Genome Annotation
            Pipeline (PGAP). Information about PGAP can be found here:
            https://www.ncbi.nlm.nih.gov/genome/annotation_prok/
            
            ##Genome-Assembly-Data-START##
            Assembly Method        :: SPAdes v. 3.11.1
            Genome Representation  :: Full
            Expected Final Version :: Yes
            Genome Coverage        :: Not Applicable
            Sequencing Technology  :: Illumina MiSeq
            ##Genome-Assembly-Data-END##
            
            ##Genome-Annotation-Data-START##
            Annotation Provider               :: NCBI
            Annotation Date                   :: 05/04/2023 04:57:10
            Annotation Pipeline               :: NCBI Prokaryotic Genome
                                                 Annotation Pipeline (PGAP)
            Annotation Method                 :: Best-placed reference protein
                                                 set; GeneMarkS-2+
            Annotation Software revision      :: 6.5
            Features Annotated                :: Gene; CDS; rRNA; tRNA; ncRNA
            Genes (total)                     :: 2,776
            CDSs (total)                      :: 2,750
            Genes (coding)                    :: 2,714
            CDSs (with protein)               :: 2,714
            Genes (RNA)                       :: 26
            tRNAs                             :: 24
            ncRNAs                            :: 2
            Pseudo Genes (total)              :: 36
            CDSs (without protein)            :: 36
            Pseudo Genes (ambiguous residues) :: 0 of 36
            Pseudo Genes (frameshifted)       :: 2 of 36
            Pseudo Genes (incomplete)         :: 32 of 36
            Pseudo Genes (internal stop)      :: 2 of 36
            ##Genome-Annotation-Data-END##
FEATURES             Location/Qualifiers
     source          1..5400
                     /organism="Micropruina sp."
                     /mol_type="genomic DNA"
                     /submitter_seqid="NODE_6830_length_5400_cov_4.840412"
                     /isolate="AMR_MDS_2195"
                     /isolation_source="Activated sludge of municipal
                     wastewater treatment plants"
                     /db_xref="taxon:2737536"
                     /environmental_sample
                     /geo_loc_name="Canada"
                     /lat_lon="49.25 N 123.1 W"
                     /collection_date="2015-06-16"
                     /metagenome_source="wastewater metagenome"
                     /note="metagenomic"
     gene            complement(<1..875)
                     /locus_tag="PLF56_06605"
     CDS             complement(<1..875)
                     /locus_tag="PLF56_06605"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_002518000.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_function: GO:0005524 - ATP binding [Evidence IEA];
                     GO_process: GO:0009058 - biosynthetic process [Evidence
                     IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="Mur ligase family protein"
                     /protein_id="HOQ53269.1"
                     /translation="MPSEMLRRRAMPAELRVADRVRLFAALSLGNVAALGSRLANRGS
                     GASIRGQVLTRIAPDAFRLLLRGRRIASVSGTNGKTTTTHLLAAAVRTGLGADAARLV
                     TNTDGANLHYGLASALSQAPSADIAVLETDERVVSDMIAHGRPEVLVLLNFSRDQLDR
                     HHEIKALGRAWRAALQRAGQAGPTVIANANDPLVVWSAGTAPKVVWVNTATLWTADAT
                     LCPACGSVLTSVDGRWDCPGCDLTQPEATWRVDGDAIVLPDGERVTPQLRVPGAFNVS
                     NAACALAAAVEMGVEV"
     gene            complement(880..1680)
                     /locus_tag="PLF56_06610"
     CDS             complement(880..1680)
                     /locus_tag="PLF56_06610"
                     /EC_number="3.1.-.-"
                     /inference="COORDINATES: protein motif:HMM:NF012863.2"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_function: GO:0016788 - hydrolase activity, acting on
                     ester bonds [Evidence IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="SGNH/GDSL hydrolase family protein"
                     /protein_id="HOQ53270.1"
                     /translation="MPQPAHPWRRYVALGDSLTEGLVDPNPDGSPRGWADRFAQHLAD
                     AAGAPVQYANLAIRGRLLRPIIAEQLEPALALKPDLVSLWGGGNDILRPRADPDAITA
                     ELERAVVRLREAGIEVILGTGIDAKDSPLLSLTRPRTAVFNANLWTIAQRHGAHVIDV
                     WGMRSLKHWGMWYDDRLHFNSVGHERVSQAALMAVGLTPRTGWDDPLPQAPAMAPREW
                     LDWQLTWARDHAGPWIGRRIRHTSSGAGRSAKFPQYVEVLPQPADPAE"
     gene            2230..2511
                     /locus_tag="PLF56_06615"
     CDS             2230..2511
                     /locus_tag="PLF56_06615"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_005936145.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="type II toxin-antitoxin system RelE/ParE family
                     toxin"
                     /protein_id="HOQ53271.1"
                     /translation="MIQSFTDETTRRVWELERAPRLGADVQRAAHKRLQMLDVARTIN
                     DLRVPPGNRLEKLVGDRGGQYSIRVNDQYRICFTWSDAGPSDVQIVDYH"
     gene            2526..2825
                     /locus_tag="PLF56_06620"
     CDS             2526..2825
                     /locus_tag="PLF56_06620"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_015069931.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_function: GO:0003677 - DNA binding [Evidence IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="HigA family addiction module antitoxin"
                     /protein_id="HOQ53272.1"
                     /translation="MDAPAHDPVTPGEILATEFLEPLGITQYRLAQATGLSQTHIGQI
                     VHGKRRITPDAGLRIARALGVSDRYWIDLQTNYDTLVAKLAHADELAAVERIVAA"
     gene            3476..3805
                     /locus_tag="PLF56_06625"
     CDS             3476..3805
                     /locus_tag="PLF56_06625"
                     /inference="COORDINATES: protein motif:HMM:TIGR01764.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_function: GO:0003677 - DNA binding [Evidence IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="helix-turn-helix domain-containing protein"
                     /protein_id="HOQ53273.1"
                     /translation="MSSMTLNPAHLSNDDWARVRAFLDSAKKRGEVVDFSTRVELLTP
                     AEVAGRLGMSRSTVLRRIADGDIKATKVGTHHRIPLNEYERYSRELMRRMAEASAVDI
                     EAELFGE"
     gene            4300..4425
                     /locus_tag="PLF56_06630"
     CDS             4300..4425
                     /locus_tag="PLF56_06630"
                     /inference="COORDINATES: ab initio
                     prediction:GeneMarkS-2+"
                     /note="Derived by automated computational analysis using
                     gene prediction method: GeneMarkS-2+."
                     /codon_start=1
                     /transl_table=11
                     /product="hypothetical protein"
                     /protein_id="HOQ53274.1"
                     /translation="MPSELMTTAGRVGPTQPAIVACYVRLPRSETAAPNYYWSLG"
     gene            4658..4987
                     /locus_tag="PLF56_06635"
     CDS             4658..4987
                     /locus_tag="PLF56_06635"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_013864650.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="hypothetical protein"
                     /protein_id="HOQ53275.1"
                     /translation="MRTVETAQTFLTASEVDALVGDYLAGMSVKALAERYGIHRATVF
                     SHLRRRNVPSRRPGLGIDEKAEAVRLARAGVSMRAIGRRMGVDRKAVRAALVESGLIV
                     DEASDDS"
     gene            5064..>5400
                     /locus_tag="PLF56_06640"
     CDS             5064..>5400
                     /locus_tag="PLF56_06640"
                     /inference="COORDINATES: ab initio
                     prediction:GeneMarkS-2+"
                     /note="Derived by automated computational analysis using
                     gene prediction method: GeneMarkS-2+."
                     /codon_start=1
                     /transl_table=11
                     /product="DNA processing protein DprA"
                     /protein_id="HOQ53276.1"
                     /translation="MTSLSDVASDERTARMVLSMLVEPNDPVTGRIMSRLGAVETLWL
                     AERDGAVVGLSPADAQVWRDHLSVPGAKELTARIDQVRQSGVRTLIPGDRDWPTALDD
                     LGEVAPYVLW"
BASE COUNT          899 a         1777 c         1859 g          865 t
ORIGIN      
        1 acctcgacgc ccatctcgac cgccgccgcc agtgcgcagg cggcgttcga cacgttgaac
       61 gcgccgggta cccgcagttg aggggtgacc cgctcgccgt ccggtagcac gatcgcgtca
      121 ccgtcgaccc gccaggtggc ctcaggctgg gtgaggtcac agccggggca gtcccagcgg
      181 ccatcgacgc tggtcagtac cgatccgcag gccgggcaca gggtcgcgtc ggcggtccac
      241 agggtcgcgg tgttcaccca caccaccttg ggggcggtgc cggccgacca caccaccaac
      301 gggtcgttcg cattggcgat cacggtgggg ccggcctgtc cggcacgctg caacgccgca
      361 cgccaggcgc gtccgagcgc cttgatctcg tggtgccggt caagctggtc acggctgaag
      421 ttgagcagca ccagcacctc gggacgtccg tgcgcgatca tgtcggacac cacgcgctcg
      481 tcggtctcga gcaccgcgat gtcggctgag ggtgcctggc tgagcgccga ggcgaggccg
      541 tagtgcaggt tcgcgccgtc ggtgttggtg accagccggg cggcgtcggc gcccaggccg
      601 gtgcggaccg cggccgccag caggtgggtg gtggtggtct tgccgttggt gccagacacg
      661 ctggcgatgc gacgtccgcg cagcagtagc cgaaaggcgt ccggggcgat gcgagtgagc
      721 acctggccac ggatcgacgc acccgaaccc cggttggcca ggcgtgagcc gagcgcggcc
      781 acgttgccca gtgacagcgc tgcgaacagc cggacgcgat cggcgacgcg cagttcggcc
      841 ggcatcgcac ggcggcggag catctcgctt ggcatgcgcc tattctgccg gatccgccgg
      901 ctggggcagc acctcgacgt actgcggaaa cttggccgag cgtccggcac ccgagctggt
      961 gtgacggatg cggcgcccga tccagggtcc ggcatggtca cgcgcccagg tgagctgcca
     1021 gtccagccac tcgcgcggcg ccattgccgg cgcttgcggc agcgggtcgt cccagccggt
     1081 gcggggggtc aggccgaccg ccatcagcgc cgcctgcgac accctctcgt ggccgacgct
     1141 gttgaagtgc agccggtcgt cgtaccacat gccccagtgc ttgaggctgc gcatgcccca
     1201 cacgtcgatc acgtgagcgc cgtgccgctg ggcgatcgtc cagagattgg cgttgaacac
     1261 tgcggtccgg ggtcgggtga gcgacagcaa cggcgaatcc ttggcgtcga tgccggtgcc
     1321 gagaatgact tcgatgccgg cctcacgcaa ccggaccacg gcgcgttcga gctcggcggt
     1381 gatcgcgtcg gggtcggcgc gtggccgaag aatgtcgttg cccccgcccc acaggctgac
     1441 cagatcgggt ttgagggcca gcgccggctc gagttgctcg gcgatgatcg gacgcagcag
     1501 tcgtccacga atggccaggt tggcgtactg gacgggcgcg ccggccgcgt cggccagatg
     1561 ctgggcgaac cggtcggccc agccgcgcgg gctgccgtcg gggttggggt cgaccaggcc
     1621 ttcggtgagg gagtcgccca gcgcgacgta gcggcgccag gggtgtgcgg gctgaggcat
     1681 ctgcatcctg ccgggtacgg gtggtgggtc gcggggtacg cggccagggg ccaacttacc
     1741 gcgcgcgccc gcggcagcgg attgcggtcc ggcctgccgc cctggtctcg tggcccgatt
     1801 ccgggcgatg agccccctcg gttcaggaga gcgctgcgct gcgcagcccc gaattgtgac
     1861 tcctctctgg acgagtcatc ggcatcgatc gagtcaggcc cctggcctgg ctcggcggca
     1921 gttgagaagc agtgacaaaa cgctgccggt tccggtgtgg ttgtggcacg gccacgaagg
     1981 aagcatcgag agatcgtgcg ggaactcgcg ctcgatcggc acgggtatgt caccaccagg
     2041 gatgccgtga tctcgggcct ccccgcgatc gtgcggccca agctcgccgc tcgcggcgga
     2101 ctggagaacg tggcccacgg cccgccgtgc aggtaagcac actctcacct gcccgcaaca
     2161 gggctgcccg gtcgacagca cggccaggtc tcgtttcgcg atactagcgt caggtgatag
     2221 cattcaggag tgatccagtc cttcactgac gagaccactc gccgggtctg ggaactggaa
     2281 cgcgcaccac gcctcggcgc cgacgtgcaa cgggccgccc acaagcggct tcagatgctc
     2341 gatgtcgccc gcaccatcaa cgatctacga gtcccgcccg gcaaccggtt ggagaagctg
     2401 gttggagacc ggggtgggca gtactcgatc cgggtcaacg accagtaccg gatctgcttc
     2461 acctggtccg acgccggccc cagcgacgtg cagatcgtgg actaccactg aaggagaaac
     2521 cgaccatgga cgcaccagca cacgaccccg tcaccccggg tgagattctc gccaccgagt
     2581 tcctggaacc gctcggcatc acccagtacc ggctcgccca ggcaaccggg ctcagccaga
     2641 cccacatcgg ccagatcgtc cacggcaagc gccgcatcac cccggatgcc gggctccgca
     2701 tcgcccgtgc cctcggggtc agcgaccgct actggatcga cctccagacg aactacgaca
     2761 ccctcgtcgc caagctcgcc cacgccgacg agctcgccgc cgtcgaacgg atcgtcgcgg
     2821 cctgagcttc agatcggcca gactggttca gcagcccaga ctcgcggtgg ctgtgatccg
     2881 acgcagatcg tgcaaggggc ccctgttgca cgaatactgc atgaagaaac ccgttccgac
     2941 tatttgaatg gtcggaatgg gtctcgagtg ggatgcctgc gtagtacgtt cgcaaacctt
     3001 accccgcacc taaagaccct ggtcacacgc tggaagcggg gcgtctaccg ggtgtctcac
     3061 cgcgcggaaa ccggcccgac ggaggactcg cggggcaccg tggttcggag gattggacac
     3121 cttcaaacct tcctcaccgc cgctgaggtc gaccgcctca ttggcgacta ccacgacggc
     3181 acgacagtca gcgagctgga agacatgtac ggcgtgcatc gcgccaccgt gtcggcgcac
     3241 ctcacgcggc gccgcgtcgg gcgtcgccct ccggggctcg gagtcgatga agccgcggag
     3301 gccgtgcggc ttcacctggg cggcgtctcg atgcgagcga tcgcgcacag gtgtggatcg
     3361 caaagccgtt cggagggttc tgatcgaagc tggtctgtcc aagtctccaa cacctggcta
     3421 gcagcgtgga cacttagtgg tgcgtaagtg gtcaacgtgg agtaggctgg ggggcatgag
     3481 cagcatgacc ctgaacccgg cgcacctgtc gaatgatgac tgggcgcgcg tgcgcgcgtt
     3541 cctggactcg gcgaagaagc gcggcgaggt cgtcgacttc tccactcggg tggagttgct
     3601 gacccccgcc gaggtcgcgg ggcggctggg gatgtctcgg tccacggtgt tgcgtcggat
     3661 cgccgacggc gacatcaagg ccacgaaggt gggcacccac catcgcatcc cgctgaacga
     3721 gtacgagcga tacagccgcg aactcatgcg ccgcatggcc gaggcgtccg ctgtcgacat
     3781 cgaggccgag ctgttcggtg agtgaagcac cactgctctt cctggacgcc aatgcgctcg
     3841 tttcgccggt gactcgcact ttgctgatcg ccggggcgcg tgcggatggt ctgcggtgga
     3901 cgtggtcgag gcacgtcgag gtcgaagccg atcggcacgc gcgcggccag gcatctcgga
     3961 catcgatcgt gcgcaggagg attctgggca cggagttgtc gccgaccgcg caggacacgg
     4021 atggtctggc tactccgctg gcgcctacga cacggcaccg ctccaggctg acccagacca
     4081 gccgagcacg attttccggg gcatggcctg cattcgctgc gaggcgcgcc ttgatgacgc
     4141 ggacggccga cagcacggac tgtgtccgga gcacctgaga ctctgacttg atctcatgcg
     4201 tcaacaccga cgacttgcgg ttacgcccgg gaagatcgga tgtcatgagt tgaaggtggc
     4261 tcgtggtgcg cctcggcccg cggtcagcga tcccgcctca tgccctctga gctgatgaca
     4321 acggccggac gggtaggccc gacgcagccg gccatcgtgg cctgctatgt gcgcttaccg
     4381 cgctccgaga cggcggcgcc gaactactac tggtcgctcg gctagaccgc tccacgacct
     4441 cgcccatccc tcacagaaaa gtgacgagat tccgacccga accagagcgg ttcctccggt
     4501 cgagggtttg aacctcgcca catcggggtg cctgagtagt acgttcggca actccgcccc
     4561 caagacaaaa accctggtca accgcctaga ccgtggggtc tacgaggtct ctcgacgtcc
     4621 ccaggatacc gctccgaagg acgatcgtgg ccctgttgtg aggactgtcg agacggctca
     4681 aacttttctc accgcctccg aggtggacgc cctcgtcggc gactacctgg ccgggatgag
     4741 cgtcaaggcg ctcgctgaga ggtacggcat ccaccgtgcc accgtgttct cccacctgcg
     4801 tcgccgcaac gtgccgagcc gacggccggg gctggggatc gacgagaagg cagaggccgt
     4861 gcgcctcgcc cgcgcaggtg tctcgatgcg agcgataggt cgccggatgg gcgtggaccg
     4921 taaggccgtg cgggcagcac tggtcgagtc gggactcatc gtggacgaag cgagtgatga
     4981 ctcgtagctg cggttccgag tagctgatcc gtcggtagcg ttcggcgcac ctcgtcggta
     5041 acgagcgccg agaggaaccg atgatgacca gcctgagtga tgtcgcaagt gacgagcgca
     5101 ccgcaaggat ggtgctgtcc atgctcgtgg aacccaatga cccggtgact ggacgcatca
     5161 tgtccaggct gggagcggtc gagacgcttt ggctcgctga gcgcgacggt gcagtggtgg
     5221 ggctgagccc tgcggacgca caggtctggc gcgaccacct cagcgtgcca ggtgcgaagg
     5281 aactgaccgc gcgtatcgac caggtgcggc aatcgggcgt tcgaacgctg atcccaggcg
     5341 atagagactg gcctaccgct ctcgacgatc ttggtgaagt cgccccctac gtgctgtgga
//
LOCUS       DAOSUG010000164         5383 bp    DNA     linear   ENV 10-JUN-2024
DEFINITION  MAG TPA_asm: Micropruina sp. isolate AMR_MDS_2195
            NODE_6853_length_5383_cov_4.140578, whole genome shotgun sequence.
ACCESSION   DAOSUG010000164 DAOSUG010000000
VERSION     DAOSUG010000164.1
DBLINK      BioProject: PRJNA925477
            BioSample: SAMN32864262
            Sequence Read Archive: SRR4236673
KEYWORDS    WGS; ENV; Metagenome Assembled Genome; MAG; Third Party Data; TPA;
            TPA:assembly.
SOURCE      Micropruina sp. (wastewater metagenome)
  ORGANISM  Micropruina sp.
            Bacteria; Bacillati; Actinomycetota; Actinomycetes;
            Propionibacteriales; Nocardioidaceae; Micropruina.
REFERENCE   1  (bases 1 to 5383)
  AUTHORS   Abdulkadir,N., Saraiva,J.P., Zhang,J., Stolte,S., Gillor,O.,
            Harms,H. and Rocha,U.
  TITLE     Genome-centric analyses of 165 metagenomes show that mobile genetic
            elements are crucial for the transmission of antimicrobial
            resistance genes to pathogens in activated sludge and wastewater
  JOURNAL   Microbiol Spectr 12 (3), e0291823 (2024)
   PUBMED   38289113
REFERENCE   2  (bases 1 to 5383)
  AUTHORS   Abdulkadir,N.
  TITLE     Direct Submission
  JOURNAL   Submitted (24-MAR-2023) Environmental Microbiology, Helmholtz
            Center for Environmental Research -UFZ, Permoserstr 15, Leipzig
            04318, Germany
COMMENT     The annotation was added by the NCBI Prokaryotic Genome Annotation
            Pipeline (PGAP). Information about PGAP can be found here:
            https://www.ncbi.nlm.nih.gov/genome/annotation_prok/
            
            ##Genome-Assembly-Data-START##
            Assembly Method        :: SPAdes v. 3.11.1
            Genome Representation  :: Full
            Expected Final Version :: Yes
            Genome Coverage        :: Not Applicable
            Sequencing Technology  :: Illumina MiSeq
            ##Genome-Assembly-Data-END##
            
            ##Genome-Annotation-Data-START##
            Annotation Provider               :: NCBI
            Annotation Date                   :: 05/04/2023 04:57:10
            Annotation Pipeline               :: NCBI Prokaryotic Genome
                                                 Annotation Pipeline (PGAP)
            Annotation Method                 :: Best-placed reference protein
                                                 set; GeneMarkS-2+
            Annotation Software revision      :: 6.5
            Features Annotated                :: Gene; CDS; rRNA; tRNA; ncRNA
            Genes (total)                     :: 2,776
            CDSs (total)                      :: 2,750
            Genes (coding)                    :: 2,714
            CDSs (with protein)               :: 2,714
            Genes (RNA)                       :: 26
            tRNAs                             :: 24
            ncRNAs                            :: 2
            Pseudo Genes (total)              :: 36
            CDSs (without protein)            :: 36
            Pseudo Genes (ambiguous residues) :: 0 of 36
            Pseudo Genes (frameshifted)       :: 2 of 36
            Pseudo Genes (incomplete)         :: 32 of 36
            Pseudo Genes (internal stop)      :: 2 of 36
            ##Genome-Annotation-Data-END##
FEATURES             Location/Qualifiers
     source          1..5383
                     /organism="Micropruina sp."
                     /mol_type="genomic DNA"
                     /submitter_seqid="NODE_6853_length_5383_cov_4.140578"
                     /isolate="AMR_MDS_2195"
                     /isolation_source="Activated sludge of municipal
                     wastewater treatment plants"
                     /db_xref="taxon:2737536"
                     /environmental_sample
                     /geo_loc_name="Canada"
                     /lat_lon="49.25 N 123.1 W"
                     /collection_date="2015-06-16"
                     /metagenome_source="wastewater metagenome"
                     /note="metagenomic"
     gene            <1..900
                     /locus_tag="PLF56_06645"
     CDS             <1..900
                     /locus_tag="PLF56_06645"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_020386282.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_function: GO:0030246 - carbohydrate binding [Evidence
                     IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="carbohydrate-binding protein"
                     /protein_id="HOQ53277.1"
                     /translation="PTVSPSGDPSGSAYRTIEAEAASQVKGGSVGSSSVSGLGNGDWL
                     RFSGLDFGSGAAANQFVAKVASGASGGVSGLVEVRLDSPTSAVIGSFAVANTGGWTSW
                     RSVPANVTGVSGVHDVYVTFTSGQPADFAAIDSLSFARPGGSADPQPTPSASVSPTVA
                     PTATSTAAGGSAYRTIEAEAASDAKGASIGSSSVSGLGNGDWLKFSGLDFGSGAAANQ
                     FVAKVASGASGGVSGLVEVRLDSPTSAVIGSFAVANTGGWTSWRSVPANVTGVSGVHD
                     VYVTFTSGQPADFVAIDSISFGR"
     gene            complement(1255..2265)
                     /locus_tag="PLF56_06650"
     CDS             complement(1255..2265)
                     /locus_tag="PLF56_06650"
                     /EC_number="3.5.4.4"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_011754149.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_function: GO:0019239 - deaminase activity [Evidence
                     IEA];
                     GO_process: GO:0009168 - purine ribonucleoside
                     monophosphate biosynthetic process [Evidence IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="adenosine deaminase"
                     /protein_id="HOQ53278.1"
                     /translation="MRELRSLPKAHLHLHFTGSMSIESLRHVGELAGKPLHDSLVDAE
                     GLLVPFNERGWFRFQRLYDMARHVVNNEAALRFIVAQAARDDAAEGSRRLELQVDPSS
                     YAPFVGGLAPALEIILDEARRASAETGVQVAIIVAASRVRHPLDARTMARLAAHHASE
                     NGVVAFGLSNNERAGTTADWEPAFRIARNAGLPGVPHAGELLGPSHVREVVEHLRPAR
                     LGHGVRSAEDPWLLEGLVAAGIALEVCPSSNVHLGVYADATDVPLRQLVEAGAQVALG
                     ADDPLLFLSRLTDQYELARTQLGFSDAELAQLARSSIRASLASTSDKEVWLAEVDAWL
                     AA"
     gene            complement(2279..2608)
                     /locus_tag="PLF56_06655"
     CDS             complement(2279..2608)
                     /locus_tag="PLF56_06655"
                     /inference="COORDINATES: protein motif:HMM:NF021496.2"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="DUF2218 domain-containing protein"
                     /protein_id="HOQ53279.1"
                     /translation="MTDTSTARVTTGRPGRYAKQLVSHMSRRAQGTYDDAGASGTVTF
                     ADAELTLTAATDALLLSLSADSDHLPRMEDVVGRHLVRFGTRDELVVEWVRSDGTAGS
                     VQRNTDD"
     gene            2756..3766
                     /locus_tag="PLF56_06660"
     CDS             2756..3766
                     /locus_tag="PLF56_06660"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_013863625.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_process: GO:0015891 - siderophore transport [Evidence
                     IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="iron-siderophore ABC transporter
                     substrate-binding protein"
                     /protein_id="HOQ53280.1"
                     /translation="MTHASPVVRRGGRLAAIALFAALLALFAGCGAAASTPSAATTRT
                     VIDVTGVPVQVPANPTRVVPLSEPTLDGVLALGVTPIGTVSGRGQSGVPNYLAEQAAG
                     VPLLGGIGQPNYEAIGAAKPDLILIDGTSVNNNQPMLEALQQIAPVVNTGYAGGEWRT
                     NFRNVAAALGRIEQGERVIADYEAAVAAARVALAPVQAKTFSIVRWQGTAPALILKEL
                     PPGQALTDLGLRRPAAQDTFGRGHSEPVSLENLPGIDADYMFFGTLGGSSVSNPNAGG
                     TADLAGAEQALKSAELVPGFTRLGAYRAQHIILVDGSAWTSTGGPLLMNRLVDDVRKA
                     LG"
     assembly_gap    3814..3913
                     /estimated_length=100
                     /gap_type="within scaffold"
                     /linkage_evidence="paired-ends"
     gene            3936..>5383
                     /locus_tag="PLF56_06665"
     CDS             3936..>5383
                     /locus_tag="PLF56_06665"
                     /inference="COORDINATES: ab initio
                     prediction:GeneMarkS-2+"
                     /note="Derived by automated computational analysis using
                     gene prediction method: GeneMarkS-2+."
                     /codon_start=1
                     /transl_table=11
                     /product="hypothetical protein"
                     /protein_id="HOQ53281.1"
                     /translation="MRTGSSPSTRAIGWVAAVVACTLVSLASGSSVTAYADETPTPTP
                     SAEPSVKATAAPADAATRKADEPAVEKSATPAKTAAAEAGSGKGTATSRAAMTVKAVT
                     SKSASAQSTAQAAARAKKTAAATAAAPRAAAVRDASAAREGIRYDLNATTSYWVPDTW
                     QAGADLVISGVGWQNAAGSAGSVIAVKLDEGGVSTTVPVLHPVTGVEQPNKTIYALVQ
                     ADAAGEWTLRVPYPTAAISTATWATGETHSLRLLSGSLLSGDVIRTAAAGFTVTDAAP
                     EPTTSPSPSSTGSPTTPAPTGSPTTPEPTEAPTAPAPTGSPTTPGPTGSPTTPAPTGS
                     PTTPAPSASPTACVPTASTPSVTVAPAAELGGTLHVTGAGFCHPSDGGSRIAVKLDEG
                     AYSRLDSTLHANRTIWAIVDASPADGTFAVDLPLPDGTTTAPGGSSPAFTAGPHTLRL
                     LTGSIKAGDTSRTLLSAPFTVASVTPSPTPTPP"
BASE COUNT          769 a         1812 c         1875 g          827 t
ORIGIN      
        1 ccgacggtgt cgccgagtgg ggatccgtcc ggttctgcat atcgcacgat cgaggcggag
       61 gctgcttcgc aggtcaaggg tggctcggtc ggttcgtcgt cggtgtcggg tttgggcaat
      121 ggtgactggt tgaggttctc gggtctggat ttcggttcgg gtgcggcggc gaatcagttt
      181 gtggcgaagg tggcttctgg tgcctcgggt ggggtgtcgg gtctggtcga ggtgcggctg
      241 gattcgccga cgtctgcggt gatcggcagt tttgcggtgg cgaacacggg tggttggacg
      301 tcgtggcgtt cggttccggc gaacgtcacc ggggtgagcg gggtgcatga cgtgtatgtc
      361 acgttcactt cgggccagcc cgccgacttc gcggcgatcg attcgctgag cttcgcccgt
      421 ccgggaggca gcgcggatcc gcagccgacg cccagcgcat cggtcagtcc gaccgtggcg
      481 ccgaccgcga cgtccacggc cgcgggaggt tctgcgtatc gcacgatcga ggcggaggcc
      541 gcttcggatg ccaagggtgc ttcgatcggt tcgtcgtcgg tgtcgggttt gggtaatggt
      601 gattggttga agttctcggg tctggatttc ggttcgggtg cggcggcgaa tcagtttgtg
      661 gcgaaggtgg cttctggtgc ctcgggtggg gtgtcgggtc tggtcgaggt gcggctggat
      721 tcgccgacgt ctgcggtgat cggcagtttt gcggtggcga acacgggtgg ttggacgtcg
      781 tggcgttcgg ttccggcgaa cgtcaccggg gtgagcgggg tgcatgacgt gtatgtcacg
      841 ttcacttcgg gccagcccgc cgacttcgtc gcgatcgaca gcatcagctt cggccgctga
      901 cccgtaggtg gggacggttc ccgttcgtgc tcaacgacgc gtagtggggc cggaccggac
      961 gtacgtgcga ctgacaaaca atgtgaagga ttgttatccg acatcgggtg acaatccttc
     1021 acgttgttgt ggagcgccgc acgactgggc ctcgtaggtg ccctcggctg gccaggtggg
     1081 cactcctgca gtgagccaga cgctccgact ggcgcacacc ccggccggcc cgcccgagcg
     1141 gtttcgacag gctcgaccag cggtagggcg ggttctcgac cagcgggggt ccggcccggc
     1201 ggggtggtct cgaccagttc gcccagcgaa cccggcctgc gtcctgcgtc ctgatcaggc
     1261 ggccagccag gcgtcgacct cggcgagcca tacctccttg tcggaggttg atgccaggct
     1321 ggcccggatg ctcgagcggg ccaactgtgc caactcggca tcgctgaaac ccaactgggt
     1381 gcgggccagc tcgtactgat cggtcagccg agagaggaac agcagcgggt cgtcggcgcc
     1441 cagcgcgacc tgggcgcccg cctcgaccag ttggcgcagc ggaacgtccg tggcgtcggc
     1501 atagacgccc aggtgcacgt tcgagctggg acagacctcg agggcgatac ccgcagcgac
     1561 cagcccctcg agcagccatg gatcctcagc cgaccgcacg ccgtgaccca gccgggcagg
     1621 ccgtaggtgc tcgaccacct cacgcacatg cgagggcccc agcaactcgc cggcatgcgg
     1681 cacgccgggt agcccggcgt tgcgggcgat ccgaaaggcc ggctcccagt cggcggtggt
     1741 gccggcccgc tcgttgttgc tcaggccgaa ggccaccacg ccgttctcgc tcgcgtggtg
     1801 ggcggccagc cgggccatgg tgcgcgcgtc cagggggtgt cgcaccctgc ttgcggcgac
     1861 gatgatcgcc acctgcaccc cggtctcggc ggacgcgcgc cgagcctcgt ccagaatgat
     1921 ctcgagagcg ggtgcgagtc cgccgacgaa cggcgcgtag ctggacggat ccacctgcag
     1981 ttcgagccgt cgcgaccctt ccgcggcgtc gtcgcgggct gcttgggcga cgatgaaccg
     2041 cagcgcggct tcgttgttca ccacgtgccg ggccatgtca tacagccgct gaaagcggaa
     2101 ccagccgcgc tcgttgaagg gcaccagcag gccctcggcg tccaccaacg agtcgtgcag
     2161 cggcttgccg gcgagctcgc cgacatgccg cagtgactcg atgctcatcg agccggtgaa
     2221 gtgcagatgc agatgcgcct tcggtaacga cctcagctcg cgcatcgacc gatggtagtc
     2281 agtcgtcggt gttgcgctgc acgctgcccg ccgtgccgtc gctgcgcacc cattcgacga
     2341 ccaattcgtc gcgggtgccg aagcgcacca ggtggcggcc gacgacatct tccatgcgcg
     2401 gcaggtggtc ggagtcggcg ctgaggctca gcagcagggc gtccgttgcg gcggtcagcg
     2461 tgagttcggc atcggcgaac gtgacggtgc cgctcgcccc ggcgtcgtca taggtgccct
     2521 gggccctgcg cgacatgtgc gagacgagtt gcttggcgta acgcccggga cgcccggtgg
     2581 tcacgcgcgc ggtcgaggta tcggtcattc caacattctc cttggttcga gacacaggct
     2641 tcatcgagag cccgatcaac tggacgctgc gtccacacag cactttgact gttaccttag
     2701 gacagcctta ccttgctatc agcagaacat tctccgtcgc caggatgtga ttcgcatgac
     2761 ccacgcgtcg cccgttgttc ggcggggcgg acgactcgcc gccatcgctc tgttcgcggc
     2821 cttgctggcg ctgttcgccg gctgcggcgc ggcggcgtcc acaccctcgg ccgcgaccac
     2881 ccgaaccgtg atcgacgtga ccggtgtgcc ggtccaggtg cccgcgaacc cgacccgggt
     2941 cgtcccgctg agcgaaccga ccctggacgg ggtgctcgcg ctcggcgtca cgccgatcgg
     3001 aacggtatcg ggccgagggc agtcgggagt gccgaactat ctggccgagc aggcggccgg
     3061 cgtgccgctg ctgggtggca tcgggcagcc caactacgag gcgatcggcg ccgccaaacc
     3121 cgacctgatc ctgatcgatg gcacgagcgt caacaacaac cagccgatgc tcgaggcgct
     3181 gcagcagatc gcgccggtgg tcaacaccgg ctatgcgggc ggcgagtggc gaaccaactt
     3241 tcgcaacgtc gccgccgcgc tggggcggat cgagcagggc gagcgggtga tcgccgacta
     3301 cgaggcggca gtcgcggcgg cccgcgttgc cctcgcaccg gtgcaggcca agacgttctc
     3361 gatcgtgcgc tggcagggca ccgcacccgc gctgatcctg aaggaactgc cgcccggcca
     3421 ggcgctgacc gacctggggt tgcgtcgtcc ggctgctcag gacacgttcg gacgcggcca
     3481 ctccgaaccg gtctcgctgg agaatctgcc gggcatcgac gccgactaca tgttcttcgg
     3541 caccctgggc ggatcgagcg tgtccaaccc caacgcgggc ggaaccgccg acctcgccgg
     3601 tgccgagcag gcgctgaaat cggcggagtt ggtgccgggc ttcacccgac tcggcgcgta
     3661 tcgcgcccaa cacatcattc tcgtcgacgg ctcggcctgg acctcaaccg ggggtccgct
     3721 gctgatgaac cggctggtcg atgacgtgcg caaggcgctc ggatgagcag gaggaatcgc
     3781 atgagaaccg gatcgtcgcc gtccacccgg gccnnnnnnn nnnnnnnnnn nnnnnnnnnn
     3841 nnnnnnnnnn nnnnnnnnnn nnnnnnnnnn nnnnnnnnnn nnnnnnnnnn nnnnnnnnnn
     3901 nnnnnnnnnn nnntcggatg agcaggagga atcgcatgag aaccggatcg tcgccgtcca
     3961 cccgggccat cgggtgggtg gcggccgtgg tggcctgcac gctggtgtcg ctcgcttcgg
     4021 ggagttcggt gaccgcctat gcggacgaga cgccgacgcc cacgccctcg gcggagccct
     4081 cggtcaaggc cacggccgcc ccggcggacg cagccaccag gaaggcggac gaacccgccg
     4141 tcgagaagag cgccacgccg gcgaagacgg ctgcagcgga ggccggctcg ggcaagggga
     4201 cggcgacctc cagggcggcg atgacggtca aagcggtgac gtccaagtcg gcgtcggccc
     4261 agtccacggc ccaggccgcg gcccgcgcga agaagaccgc ggccgcgacc gcggcggcgc
     4321 cccgggcggc tgccgtgcgg gatgcctccg ccgcgcgcga gggcatccgc tacgacctga
     4381 acgccaccac ctcgtactgg gtaccggaca cctggcaggc cggcgccgac ctggtgatca
     4441 gcggcgtcgg ctggcagaac gccgccgggt cggcgggctc ggtcatcgcg gtgaagctcg
     4501 atgagggagg ggtgtcgacc acggttccgg tgctgcatcc ggtgaccggg gtggagcagc
     4561 cgaacaagac catctacgcc ctcgtgcagg ccgacgccgc tggcgagtgg acgctgcgcg
     4621 tgccctaccc gaccgcggcc atcagcaccg ccacctgggc cactggcgaa acgcactcgt
     4681 tgcgcctgct gagcggctcg ctgctcagcg gtgacgtgat ccgcaccgct gccgccgggt
     4741 tcacggtcac cgacgccgcg ccggagccga ctacgtcgcc gtcgccgagc tcgaccggct
     4801 cgcccaccac cccggccccg accggctcgc ccacgacccc ggagccgacc gaggcgccca
     4861 ccgcgccggc gccgaccggg tcgcccacca ccccggggcc gaccgggtcg cccaccaccc
     4921 cggccccgac cggctcgccc accaccccgg ccccgagcgc gtcaccgacg gcctgcgtgc
     4981 cgacggcgtc cacaccgtcg gtgacggtgg ctccggccgc cgagctgggc ggcacgctgc
     5041 acgtgaccgg tgccggcttc tgccacccga gcgacggcgg ttcgcggatc gcggtcaaac
     5101 tcgatgaggg cgcctacagc cggctcgact cgacgctgca cgccaaccgg acgatctggg
     5161 cgatcgtgga cgcctcgccc gccgacggca cgttcgcggt ggacctgccc ctgccggacg
     5221 gaaccaccac ggcacccggc ggctcatccc ccgcgttcac cgccggtccg cacaccctgc
     5281 gcctgctgac cggttcgatc aaggccggcg acacctcgcg cacgctgctg tccgcaccgt
     5341 tcaccgtggc ctcggtgacg ccgtccccga cccccacccc ccc
//
LOCUS       DAOSUG010000165         5369 bp    DNA     linear   ENV 10-JUN-2024
DEFINITION  MAG TPA_asm: Micropruina sp. isolate AMR_MDS_2195
            NODE_6868_length_5369_cov_4.488897, whole genome shotgun sequence.
ACCESSION   DAOSUG010000165 DAOSUG010000000
VERSION     DAOSUG010000165.1
DBLINK      BioProject: PRJNA925477
            BioSample: SAMN32864262
            Sequence Read Archive: SRR4236673
KEYWORDS    WGS; ENV; Metagenome Assembled Genome; MAG; Third Party Data; TPA;
            TPA:assembly.
SOURCE      Micropruina sp. (wastewater metagenome)
  ORGANISM  Micropruina sp.
            Bacteria; Bacillati; Actinomycetota; Actinomycetes;
            Propionibacteriales; Nocardioidaceae; Micropruina.
REFERENCE   1  (bases 1 to 5369)
  AUTHORS   Abdulkadir,N., Saraiva,J.P., Zhang,J., Stolte,S., Gillor,O.,
            Harms,H. and Rocha,U.
  TITLE     Genome-centric analyses of 165 metagenomes show that mobile genetic
            elements are crucial for the transmission of antimicrobial
            resistance genes to pathogens in activated sludge and wastewater
  JOURNAL   Microbiol Spectr 12 (3), e0291823 (2024)
   PUBMED   38289113
REFERENCE   2  (bases 1 to 5369)
  AUTHORS   Abdulkadir,N.
  TITLE     Direct Submission
  JOURNAL   Submitted (24-MAR-2023) Environmental Microbiology, Helmholtz
            Center for Environmental Research -UFZ, Permoserstr 15, Leipzig
            04318, Germany
COMMENT     The annotation was added by the NCBI Prokaryotic Genome Annotation
            Pipeline (PGAP). Information about PGAP can be found here:
            https://www.ncbi.nlm.nih.gov/genome/annotation_prok/
            
            ##Genome-Assembly-Data-START##
            Assembly Method        :: SPAdes v. 3.11.1
            Genome Representation  :: Full
            Expected Final Version :: Yes
            Genome Coverage        :: Not Applicable
            Sequencing Technology  :: Illumina MiSeq
            ##Genome-Assembly-Data-END##
            
            ##Genome-Annotation-Data-START##
            Annotation Provider               :: NCBI
            Annotation Date                   :: 05/04/2023 04:57:10
            Annotation Pipeline               :: NCBI Prokaryotic Genome
                                                 Annotation Pipeline (PGAP)
            Annotation Method                 :: Best-placed reference protein
                                                 set; GeneMarkS-2+
            Annotation Software revision      :: 6.5
            Features Annotated                :: Gene; CDS; rRNA; tRNA; ncRNA
            Genes (total)                     :: 2,776
            CDSs (total)                      :: 2,750
            Genes (coding)                    :: 2,714
            CDSs (with protein)               :: 2,714
            Genes (RNA)                       :: 26
            tRNAs                             :: 24
            ncRNAs                            :: 2
            Pseudo Genes (total)              :: 36
            CDSs (without protein)            :: 36
            Pseudo Genes (ambiguous residues) :: 0 of 36
            Pseudo Genes (frameshifted)       :: 2 of 36
            Pseudo Genes (incomplete)         :: 32 of 36
            Pseudo Genes (internal stop)      :: 2 of 36
            ##Genome-Annotation-Data-END##
FEATURES             Location/Qualifiers
     source          1..5369
                     /organism="Micropruina sp."
                     /mol_type="genomic DNA"
                     /submitter_seqid="NODE_6868_length_5369_cov_4.488897"
                     /isolate="AMR_MDS_2195"
                     /isolation_source="Activated sludge of municipal
                     wastewater treatment plants"
                     /db_xref="taxon:2737536"
                     /environmental_sample
                     /geo_loc_name="Canada"
                     /lat_lon="49.25 N 123.1 W"
                     /collection_date="2015-06-16"
                     /metagenome_source="wastewater metagenome"
                     /note="metagenomic"
     gene            <1..316
                     /locus_tag="PLF56_06670"
     CDS             <1..316
                     /locus_tag="PLF56_06670"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_016667533.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_function: GO:0003677 - DNA binding [Evidence IEA];
                     GO_function: GO:0003916 - DNA topoisomerase activity
                     [Evidence IEA];
                     GO_function: GO:0005524 - ATP binding [Evidence IEA];
                     GO_process: GO:0006265 - DNA topological change [Evidence
                     IEA]"
                     /codon_start=2
                     /transl_table=11
                     /product="DNA gyrase C-terminal beta-propeller
                     domain-containing protein"
                     /protein_id="HOQ53282.1"
                     /translation="SGCCARTSTAPGSSSALPGTDVGSVKVTPFAEYPAKGRGTGGVR
                     CQRFLKGEDVLQLAWVGATPVVAAAASGSPVELPAIDPRRDASGTSVGQPIAAVSTRQ
                     HS"
     gene            complement(486..971)
                     /locus_tag="PLF56_06675"
     CDS             complement(486..971)
                     /locus_tag="PLF56_06675"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_008361809.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="carbonic anhydrase"
                     /protein_id="HOQ53283.1"
                     /translation="MFDDLLAANARYSTQAPRGFDGYAHAGVAMVTCMDSRLQPLEMI
                     GLFLGEAKILRTPGGHVTEDALNGCVLGVNLLRVSRILVVQHTRCAMASGTDEDVRRR
                     VAEASGRSADQLTVGADPDQAGRLRRDVTTVREHPLITGRAEVGGFIYDVDSGLLTQV
                     V"
     gene            1423..1641
                     /locus_tag="PLF56_06680"
     CDS             1423..1641
                     /locus_tag="PLF56_06680"
                     /inference="COORDINATES: ab initio
                     prediction:GeneMarkS-2+"
                     /note="Derived by automated computational analysis using
                     gene prediction method: GeneMarkS-2+."
                     /codon_start=1
                     /transl_table=11
                     /product="hypothetical protein"
                     /protein_id="HOQ53284.1"
                     /translation="MTTTEINTTPITATELSSLSMIDRVALHCGLAMISWAHRPQRTR
                     RTATADTMAEVEHRRELIDAQYRVHALR"
     gene            complement(1781..2821)
                     /locus_tag="PLF56_06685"
     CDS             complement(1781..2821)
                     /locus_tag="PLF56_06685"
                     /inference="COORDINATES: protein motif:HMM:NF017072.2"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="hypothetical protein"
                     /protein_id="HOQ53285.1"
                     /translation="MSGVIVLRCEERASERRTPITPQGAAQLQQQGVTVLVESSPVRV
                     FADDEYRAAGVAVVPAGSWATASRDAVVVGIKELPDEPEPLRHRHVYFAHAFKGQRDA
                     AHTLRRFVDGGGELFDLEYLVDDDGRRVVAFGYWAGFVGGSLAALQLAGTLRAPLQPM
                     TLDGLTTALEQAAQKLVGATCVVTGAFGRAGRGATDALILADVGVSRWDRDDTAELDS
                     NALVAHDALVHCVHADQPQRPFLTEADRDRPGRRLRVLADVTADVTSDLNLLPVSDRH
                     TSWPEPVRRIWTDPVPLDAIVIDNLPSLLPLQASADFSRALVPLLPSLFTDGPVWSRA
                     RRRFELAVEGLG"
     gene            complement(2818..4905)
                     /locus_tag="PLF56_06690"
     CDS             complement(2818..4905)
                     /locus_tag="PLF56_06690"
                     /EC_number="5.6.2.2"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_002513676.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_function: GO:0003677 - DNA binding [Evidence IEA];
                     GO_function: GO:0003918 - DNA topoisomerase type II
                     (double strand cut, ATP-hydrolyzing) activity [Evidence
                     IEA];
                     GO_function: GO:0005524 - ATP binding [Evidence IEA];
                     GO_process: GO:0006265 - DNA topological change [Evidence
                     IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="DNA topoisomerase IV subunit B"
                     /protein_id="HOQ53286.1"
                     /translation="MTIKGKPAAEGRDYEARHLLVLEGLEAVRKRPAMYIGSTDTRGL
                     MHCLWEIIDNAVDEALGGFGDTIDVALNADGSIVVKDVARGIPVDIEPRTGLSGVEVV
                     FTKLHAGGKFGAGSYNATGGLHGVGASVVNALSARLDVEVDRNSATWALSFRRGVPGT
                     FDGDGPDAPFTPGAQLSKVGRAARGRTGTRVRYWPDRQIFLPDAELSWTSLVARARQT
                     SFLVPGLRLAVTDARASEPVSEVFQHDGGISEFCDFLATDAPVTEVLRLQGTDSFTET
                     VPMLDADGAMTPSDVERTLDVDIALRWGAGYDTTVQSFVNIIATPKGGTHVQGFERGL
                     VRAFAKALEGTRLLKAGDEVLKDDVIEGMTAVVTVRLAEPQFEGQTKEVLGTPPVTRL
                     VARVVESELVDFLTSNRAAQRPQARAVMEKVVSASRTRVAARAHKENQRRKNALESST
                     LPAKLADCRSTDAERTELFIVEGDSAMGTAKLARNSEFQALLPIRGKILNVQRASVGD
                     MLKNAECASIIQVVGAGSGRTFELEQARYGKVIFMADADSDGAHIRTLLATLFFKYMR
                     PMVEAGRVFTAVPPLHCFELTNPRKGQERYLYTYSEGEYQRKLAELTKRGVGFKEPQR
                     YKGLGEMDADQLKETTMDPRHRTLRRITVDEGESLELAMATFEKLMGNDVAPRKEFIV
                     AGAYALDAERIDA"
BASE COUNT          822 a         1897 c         1780 g          870 t
ORIGIN      
        1 gtcggggtgc tgcgctcgga cctctacggc acccggatcg tccagcgcac tgcccggcac
       61 ggacgtcggc agcgtcaagg tgacaccttt cgccgagtac ccggccaagg gtcgcggcac
      121 cggcggcgtg cgctgccagc gcttcctcaa gggcgaggac gtgttgcagc tggcctgggt
      181 gggcgccacc ccggtggtgg ccgcggcggc gtccggatca ccggtggagt tgcctgccat
      241 cgacccgcgg cgcgacgcgt ccggcacgtc ggtgggccag ccgatcgccg cggtctcgac
      301 ccggcagcac agctgaccgg ctgacccagc ctgtcgacac cgctggtggc gcctgtcgac
      361 acccggcacg ccgagcgcca tgacgttgcg cggtcaaggg ctggttgcgc gggttcgtac
      421 gcgcgcaatg agcgctacgc cgcgcgacgt cagccggcgg cgggtccaga ccggtctaga
      481 ccggtctaga ccacctgggt gagcaggccg ctgtccacgt cgtagatgaa gccacccacc
      541 tccgcgcggc ccgtgatcag aggatgctca cgcacggtgg tcacgtcgcg tcgcagacgg
      601 cccgcctggt cggggtcggc gccgaccgtg agctgatcgg cgctgcgtcc gctggcctcg
      661 gcgacccggc gccggacgtc ttcgtcggta ccggacgcca tggcgcagcg cgtgtgctgg
      721 acgaccagga ttcggctgac ccgcagcagg ttcaccccca gaacgcagcc gttcagcgcg
      781 tcctcggtga catggccgcc gggggtgcgc aggatcttcg cctcaccgag gaacagcccg
      841 atcatctcca ggggctgcag ccgcgagtcc atgcacgtga ccatggccac ccctgcgtgg
      901 gcgtacccgt cgaagccgcg aggggcctgg gtggaatagc gagcgttggc cgccagcaga
      961 tcgtcgaaca tgcgcacacc ctaaccggcc gttgcgcccg cgccgaagcg tccagcacgt
     1021 gacgcgcgcg caggccgcac tcgccaccat gcaggcgcac accgtcaatc gccgcaatgc
     1081 cccacgcagt tgtatttttt ggcggacagg tttatcttct atcgtggccg caaccactga
     1141 aagggggccg actttgatga ctgttcgcat tctgcgcact gctggcgctg acgcgtcgcg
     1201 ggtcggtccg ttgctcgcgc cgcgccgtca cttctgctga ctggccgttg ccgagtcttc
     1261 ggcgcccgcc gcttcctgac aagggtggac gccgcacgct cgtgacctga gccgccggcg
     1321 tccattatcg aaccacgcgt tcgccactgt cacgtgcgat tctcacaatg tcgccacgtg
     1381 atgattcgtc atgtcttctt cacctgcacc aaagggtcca tgatgaccac caccgaaatc
     1441 aacaccacac ctattaccgc gaccgaactg tccagtttga gcatgatcga ccgggtcgca
     1501 ttgcactgtg gactggccat gatcagctgg gcacaccgcc cccagcgcac ccgccgcacc
     1561 gcgaccgccg acaccatggc cgaggtcgag caccggcgtg aactgatcga cgcccagtac
     1621 cgcgtgcatg cgctgcggtg agcggcccgc gcaccgccgg cgagacgctc gccggtgcgc
     1681 gggacctccg cgccgcatca actccgttca gcgcccgtca aaccctgtgc acaccccgtc
     1741 gcgcgcgagc gtcgcgatgc acccgatcag ttagggcgtg tcagcccagg ccctcgaccg
     1801 cgagctcgaa gcgtcgccgg gcccggctcc acaccggccc gtcggtgaac aggctgggca
     1861 gcaacggcac cagggcccgc gagaagtccg cgctggcctg gagcggcaac agcgacggca
     1921 ggttgtcgat cacgatcgca tccagcggca ccgggtcggt ccagatgcgg cgcacgggct
     1981 cgggccagct ggtgtgccgg tcgctgaccg gcagcaggtt cagatcggag gtgacatcgg
     2041 cggtcacgtc ggccagcacc cgcagccggc gaccgggccg atcacgatcg gcttcggtca
     2101 gaaacggacg ctgcggttga tcggcgtgca cgcagtgcac cagcgcgtca tgggccacca
     2161 gcgcgttcga gtcgagttcg gcggtgtcgt cacggtccca gcgggatacg cccacgtcgg
     2221 ccaggatcag cgcgtcggtg gcgccacgcc cggcgcgtcc gaacgcgccg gtgaccacgc
     2281 aggtggcgcc gaccagcttc tgcgcagcct gttccagcgc agtggtgaga ccgtcgagcg
     2341 tcatcggctg cagcggcgcc cgcagggtac cggcgagctg cagtgccgcc aacgagccgc
     2401 cgacgaaccc cgcccagtag ccgaacgcca ccacgcggcg tccatcgtcg tcgaccaagt
     2461 actccaggtc gaaaagttca ccaccgccgt cgacgaagcg tcgcagggtg tgcgcggcgt
     2521 cgcgctggcc cttgaaggcg tgcgcgaagt acacgtgccg gtgccgcagc ggttctggct
     2581 cgtcgggtag ctccttgatg ccgaccacga ccgcgtcacg gctcgcggtg gcccacgaac
     2641 cggccgggac caccgcgacg cccgccgccc gatactcgtc gtcggcgaat acccgtaccg
     2701 gcgacgactc gaccagcacg gtgaccccct gctgctgcag ttgggcagcg ccctgcggag
     2761 tgatcggggt gcgtcgctcg ctggcgcgct cttcgcagcg cagcacgatc acgccgctca
     2821 tgcgtcgatc cgctcggcat cgagcgcgta ggcgccggcc acgatgaact ctttgcgcgg
     2881 cgccacgtcg ttacccatca gcttttcgaa ggtggccatc gccaactcga gcgattcgcc
     2941 ctcgtcgacg gtgatgcggc gcagtgtgcg gtgccggggg tccatggtgg tttccttgag
     3001 ctggtcggcg tccatttcgc cgagaccctt gtagcgttgc ggctctttga aacccacgcc
     3061 gcgcttggtc agttcggcca gctttcgctg atactcgccc tcgctgtagg tgtacaggta
     3121 gcgctcctgc cccttgcgcg gattggtcag ttcgaagcag tgcaacggcg gcaccgcggt
     3181 gaacacccgg ccggcctcga ccatcggccg catgtacttg aagaacaagg tggccagcag
     3241 ggtgcggatg tgggccccgt cggagtcggc gtccgccatg aagatcacct tgccgtaacg
     3301 ggcctgctcg agctcgaacg tgcgtccgga ccccgccccg accacctgaa tgatcgaagc
     3361 gcactcggcg ttcttgagca tgtcgccgac gctggcgcgc tgcacgttga gaatcttgcc
     3421 gcgaatcggc agcaacgcct gaaactccga gttgcgagcc agtttggccg tgcccatcgc
     3481 cgagtcgccc tcgacgatga acagctcggt gcgctcagca tcggtgctgc ggcagtcggc
     3541 cagcttggcc gggagtgtgc tcgactcgag ggcgttcttg cggcgctgat tctctttgtg
     3601 cgcgcgggcc gccactcggg tgcgtgaggc cgacaccacc ttctccatca cggcgcgcgc
     3661 ctgcggacgc tgcgcggccc ggttcgaggt gagaaagtcg accagctcgc tctcgaccac
     3721 ccgggccacc aggcgggtca ccggcggggt gccgagcacc tctttggtct ggccctcgaa
     3781 ctgcggctcg gccaaccgca cggtgaccac cgccgtcatg ccctcaatga cgtcgtcttt
     3841 gagcacctcg tcgcccgcct tgagcagccg ggtgccttcc aacgccttgg cgaaggcgcg
     3901 caccaggccg cgctcgaagc cctgcacgtg ggtgccgccc ttgggcgtgg cgatgatgtt
     3961 gacgaacgac tgcacggtgg tgtcgtaacc ggcgccccac cgcagcgcaa tgtcgacgtc
     4021 gagggtgcgc tccacgtcgc tgggggtcat ggctccgtcg gcgtcgagca tcggcacggt
     4081 ttcggtgaac gagtcggtgc cctgcagtcg cagcacctcg gtgaccgggg cgtcggtggc
     4141 cagaaagtcg cagaactccg agatgccgcc gtcgtgctga aacacctcgc tgaccggctc
     4201 ggacgcccgc gcgtcggtga ccgccaagcg caaccccggc accagaaagc tggtctgccg
     4261 ggcccgcgcc accagcgaag tccacgacag ctcggcgtcg ggcagaaaga tctgccggtc
     4321 gggccagtac cgcacccggg taccggtacg accgcgggcg gcgcggccca ccttggacag
     4381 ctgcgcgccg ggagtgaacg gagcgtccgg gccgtcgccg tcgaacgtgc cgggcacacc
     4441 acgccggaag gacagcgccc aggtggcgct gttgcggtcc acctcgacat cgagccttgc
     4501 cgacagcgcg ttcaccacgg acgcgcccac accgtgcagt ccgccggtgg cgttgtaaga
     4561 accggccccg aacttgccgc cggcatgcag cttggtgaac accacctcga ccccggacag
     4621 gccggtgcgc ggctcgatgt cgaccggaat gccgcgcgcg acgtctttga ccacgatgct
     4681 gccgtcggcg ttcagcgcga cgtcgatggt gtcgccgaag ccgcccagcg cctcgtcgac
     4741 cgcgttgtcg atgatttccc acaggcagtg catcaggccc cgggtatcgg tggagccgat
     4801 gtacatggcg ggccgtttac gcaccgcctc caggccctcg agcacgagca ggtggcgggc
     4861 ctcgtagtcg cggccctcgg ccgcaggctt gcctttgatg gtcacgcgag caggctagcg
     4921 ccacgctctg acaagaacgt ggatctcagc ccggatccac ccgatctcca cccgctgcga
     4981 gaccccgtcc gcgccccctg gcgtggccgg ccattctcaa caggcgaccg ggccatcgca
     5041 tgctcatggt cggcgacctt gacccgccac gcgcggggaa cgaacgctcg gcaccgccgc
     5101 tgtaggttct cgcgtgagcc gtcgtccgct ttgggccagt gacaagagct gcgacaagtt
     5161 gttggccctc ctgggttctc ttcatcttgt cggattccgt acccgttgtc gctggctact
     5221 tgcgtgacaa ctgatgcggg ccacctgcat tcttcccggc cagagcctgc gctcgcaccg
     5281 cctcagaggg ctgaaaccca gctgcgacgg ttcacctagt ggcccgtttc ggaggttgtt
     5341 gagggtttgg ctttcttgag gacgtggtc
//
LOCUS       DAOSUG010000166         5367 bp    DNA     linear   ENV 10-JUN-2024
DEFINITION  MAG TPA_asm: Micropruina sp. isolate AMR_MDS_2195
            NODE_6871_length_5367_cov_4.884789, whole genome shotgun sequence.
ACCESSION   DAOSUG010000166 DAOSUG010000000
VERSION     DAOSUG010000166.1
DBLINK      BioProject: PRJNA925477
            BioSample: SAMN32864262
            Sequence Read Archive: SRR4236673
KEYWORDS    WGS; ENV; Metagenome Assembled Genome; MAG; Third Party Data; TPA;
            TPA:assembly.
SOURCE      Micropruina sp. (wastewater metagenome)
  ORGANISM  Micropruina sp.
            Bacteria; Bacillati; Actinomycetota; Actinomycetes;
            Propionibacteriales; Nocardioidaceae; Micropruina.
REFERENCE   1  (bases 1 to 5367)
  AUTHORS   Abdulkadir,N., Saraiva,J.P., Zhang,J., Stolte,S., Gillor,O.,
            Harms,H. and Rocha,U.
  TITLE     Genome-centric analyses of 165 metagenomes show that mobile genetic
            elements are crucial for the transmission of antimicrobial
            resistance genes to pathogens in activated sludge and wastewater
  JOURNAL   Microbiol Spectr 12 (3), e0291823 (2024)
   PUBMED   38289113
REFERENCE   2  (bases 1 to 5367)
  AUTHORS   Abdulkadir,N.
  TITLE     Direct Submission
  JOURNAL   Submitted (24-MAR-2023) Environmental Microbiology, Helmholtz
            Center for Environmental Research -UFZ, Permoserstr 15, Leipzig
            04318, Germany
COMMENT     The annotation was added by the NCBI Prokaryotic Genome Annotation
            Pipeline (PGAP). Information about PGAP can be found here:
            https://www.ncbi.nlm.nih.gov/genome/annotation_prok/
            
            ##Genome-Assembly-Data-START##
            Assembly Method        :: SPAdes v. 3.11.1
            Genome Representation  :: Full
            Expected Final Version :: Yes
            Genome Coverage        :: Not Applicable
            Sequencing Technology  :: Illumina MiSeq
            ##Genome-Assembly-Data-END##
            
            ##Genome-Annotation-Data-START##
            Annotation Provider               :: NCBI
            Annotation Date                   :: 05/04/2023 04:57:10
            Annotation Pipeline               :: NCBI Prokaryotic Genome
                                                 Annotation Pipeline (PGAP)
            Annotation Method                 :: Best-placed reference protein
                                                 set; GeneMarkS-2+
            Annotation Software revision      :: 6.5
            Features Annotated                :: Gene; CDS; rRNA; tRNA; ncRNA
            Genes (total)                     :: 2,776
            CDSs (total)                      :: 2,750
            Genes (coding)                    :: 2,714
            CDSs (with protein)               :: 2,714
            Genes (RNA)                       :: 26
            tRNAs                             :: 24
            ncRNAs                            :: 2
            Pseudo Genes (total)              :: 36
            CDSs (without protein)            :: 36
            Pseudo Genes (ambiguous residues) :: 0 of 36
            Pseudo Genes (frameshifted)       :: 2 of 36
            Pseudo Genes (incomplete)         :: 32 of 36
            Pseudo Genes (internal stop)      :: 2 of 36
            ##Genome-Annotation-Data-END##
FEATURES             Location/Qualifiers
     source          1..5367
                     /organism="Micropruina sp."
                     /mol_type="genomic DNA"
                     /submitter_seqid="NODE_6871_length_5367_cov_4.884789"
                     /isolate="AMR_MDS_2195"
                     /isolation_source="Activated sludge of municipal
                     wastewater treatment plants"
                     /db_xref="taxon:2737536"
                     /environmental_sample
                     /geo_loc_name="Canada"
                     /lat_lon="49.25 N 123.1 W"
                     /collection_date="2015-06-16"
                     /metagenome_source="wastewater metagenome"
                     /note="metagenomic"
     gene            98..889
                     /locus_tag="PLF56_06695"
     CDS             98..889
                     /locus_tag="PLF56_06695"
                     /EC_number="3.1.21.2"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_017933517.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_function: GO:0003677 - DNA binding [Evidence IEA];
                     GO_function: GO:0008270 - zinc ion binding [Evidence IEA];
                     GO_process: GO:0006281 - DNA repair [Evidence IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="deoxyribonuclease IV"
                     /protein_id="HOQ53287.1"
                     /translation="MIAMGAHVDQTDPIAEATSRGAQLSQFFLGDPQGWKGPKVAYAG
                     GAAALKADAEAAGIALYVHAPYVLNVASTNNRIRIPSRQHLQKQLTAAAEIGAAGVIV
                     HGGHLAKDEDPVVGYTNWFKAVDGLDIAVPLLIENTAGGTNAMARQLESIDRLWAAIG
                     ASANVDMVGFCLDTCHAHAGGNELFGLVDRIKAITGRVDLIHANDSRDSFDSGADRHA
                     NWGTGQCDPDGVADVLRSADAPVVIETPGGVAEHVRDLTWLVERL"
     gene            1661..3724
                     /gene="pta"
                     /locus_tag="PLF56_06700"
     CDS             1661..3724
                     /gene="pta"
                     /locus_tag="PLF56_06700"
                     /EC_number="2.3.1.8"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_014845432.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_function: GO:0016407 - acetyltransferase activity
                     [Evidence IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="phosphate acetyltransferase"
                     /protein_id="HOQ53288.1"
                     /translation="MTRSIYVAAPEGQTGKSVIALGVLDTLLREVESVGVFRPLVRAG
                     EPDDILETLLAQPGVDQSYDDAVGITYDEMRADFDSALETIVGKFGRISPRFDAIVVL
                     GSDYADVTSPTELAFNAEIAANLNSSLLLVVSGFDRDAQQIRAAAESALNEFTAHHVQ
                     TIGVIANRVEPSILNETVAELHGLTDLLVAALPAEKVLVAPTLRAQYEAVGAVHVLGN
                     RDLIERESQDVVVAAMTLPNILSRLVTEATVLVPSDRSDLLPGLILAHHSGSFPQLTG
                     VIMTGGYPIPDAVVRLFDGMHLDLPIGMTDLGTYTTAKKVFNTQGHFTDSPRKVEISR
                     RLFSENVDADALIRAIEVEPSRAVTPLMFGYQLNELARRDRRRIVMPESTDDRILESA
                     SILLQRGVADLTLLGDPVEIKTRASAMGWVLDGVQIVNPTDPALLDRFSNEYAKLRAH
                     KGVTVGQAREKMKDLSYFGTMMVHLGMADGMVSGAINTTANTIRPSLEFIKTKPGVKV
                     VSGSFLMCMPDRVHVYADCAVNPDPSAEQLADIAISSAETALAFGIEPRVAMLSYSTG
                     TSGSGADVDKVRQATDLVRERRPDLALEGPIQFDAAVDPAVAKTKLPESAVAGQATVF
                     IFPDLNTGNNTYKAVQRSSGAVAIGPILQGLKKAVNDLSRGALVDDIVNTVVITAIQA
                     QADAG"
     gene            3768..4952
                     /locus_tag="PLF56_06705"
     CDS             3768..4952
                     /locus_tag="PLF56_06705"
                     /EC_number="2.7.2.1"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_016455167.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_function: GO:0000287 - magnesium ion binding [Evidence
                     IEA];
                     GO_function: GO:0005524 - ATP binding [Evidence IEA];
                     GO_function: GO:0008776 - acetate kinase activity
                     [Evidence IEA];
                     GO_process: GO:0006085 - acetyl-CoA biosynthetic process
                     [Evidence IEA];
                     GO_process: GO:0016310 - phosphorylation [Evidence IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="acetate kinase"
                     /protein_id="HOQ53289.1"
                     /translation="MSTPILLLNAGSSSIKYQVIDADNEDVLASGIIQRIGDESGTID
                     HRVDGQTHHQTRGFPNHGRALTVLVQMFDEFGPKLASVKAVGHRIVHGGSLFNSTTVI
                     NDEVIEHLREVSPLAPLHNPPGIAGIEAAMRSLPDVPHVAIFDTAFFSTLPAEAYTYA
                     IPHELAAEHGIRKYGFHGTSHSFVSRKVAEVLDKPYDSFNQIVCHLGNGASISAIANG
                     VAVEASMGLTPLAGLVMGTRSGDVDPGLHAFLARQVGMSIDEIDNTLNKKSGMLGLSG
                     VTDFRDINDLIAEGDAGAVLALDVYVHRLLSYIGSYIAILGGVEAITFTAGVGENNPT
                     VRAAIATRLAFLGVRLDEAANEARSKEPRAISTPESSIAILVVPTNEELAMARETKAA
                     IS"
     gene            complement(5087..>5367)
                     /locus_tag="PLF56_06710"
     CDS             complement(5087..>5367)
                     /locus_tag="PLF56_06710"
                     /inference="COORDINATES: protein motif:HMM:NF013970.2"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=3
                     /transl_table=11
                     /product="PIN domain-containing protein"
                     /protein_id="HOQ53290.1"
                     /translation="AIQEYLFHGLRVSDRESAIAQSRALVGVLHLHDLTGAVLLRAIQ
                     LVEATPLRGRDAVHAATALEVGFDAIVTLDSDFALVPGLRALHPSEAL"
BASE COUNT          924 a         1922 c         1721 g          800 t
ORIGIN      
        1 gccgggatct ctgcctgcgc cgagatcccg ggacaaagcc gggatgacga gcaccacgct
       61 ggtcgagact gtcggggcac ccggttaggc tcggcccatg atcgccatgg gtgcccacgt
      121 cgaccagacc gacccgattg ccgaggcgac gtcgcgcggg gcacaactca gccagttctt
      181 tctcggcgac ccgcagggct ggaagggccc caaggtcgcc tacgccggcg gcgccgccgc
      241 gctgaaggcc gacgccgaag cggccggtat cgcgctctac gtgcacgcgc cgtacgtgct
      301 caacgtggcc agcaccaaca accgcatccg cattccgtcg cgtcagcatc tgcagaagca
      361 gctcacagcc gcggccgaga tcggggcggc cggcgtcatc gtccacggcg gtcatctcgc
      421 taaggacgaa gatcccgtgg tcggctacac caactggttc aaggccgtgg acggcctcga
      481 catcgccgtg ccgttgttga tcgagaacac cgccggcggc acgaacgcga tggcccggca
      541 gctcgagagc atcgaccggt tgtgggccgc catcggcgcc tcggccaacg tcgacatggt
      601 cggcttctgc ctcgacacct gccacgccca cgccggcggc aatgagctgt tcggtctggt
      661 cgaccgcatc aaggcgatca ccggacgggt cgacctgatc cacgccaacg actcccgcga
      721 ctccttcgac tccggggcgg accggcacgc caactggggc accggtcagt gcgaccccga
      781 cggagtcgcc gacgtgctgc gcagcgcaga tgccccggtg gtgatcgaga ctcccggcgg
      841 cgtcgccgag cacgtccgtg acctgacctg gctggtcgag cgactctgaa tccctccggc
      901 acaagcacgt ccgcgacctg gctggcccac cggctctgag ccgccagtat gagcacggcc
      961 aagcggcctg accgagaagt ggcgacgcca tcgcgcgaga agcgcccatg gcccgtggca
     1021 gccgcgcagg actgcaccac atttggctcg ctcccacgct caccaaggcg gacgcgcccg
     1081 caaatgtggt gcagatttgc gcgcctcgcg cttggaacct cgccgtgccc gaggtcttgc
     1141 gcaacccggc gaccctgaaa cgtggggacg gtgccggcga ccgacccaat ccgaaccacg
     1201 ccgtgcccga agcagccgtg tagaactgca ccacatttgg ctccctccca cgctcaccaa
     1261 ggcggacgcg cccgcaaatg tggtgcagat ttgcgcacca cgcgctcgaa accccgccat
     1321 gcccgaggtc tcgcgcaacc cggcgaccct gaaacgtggg gacggtgccg gcgaccgacc
     1381 caatccgaac cacgccgtgc ccgaagcagc cgtgtagaac tgcaccacat ttggctccct
     1441 cccacgctca ccaaggcgga cgcccccgca actgtggtgc agatttgcat gccaaccggc
     1501 ccgaacctcg ccgcgcccag gggtgtcgca acccgaagac gatgacacct ggacggcgcc
     1561 agcgaccggc gacccgaccg ttgcctcgtc caaccaccca cccctgcccg cgaaccgggc
     1621 acggcagaca gggcccagcg gctggtttag cctgagaacc gtgactcgaa gcatctacgt
     1681 agctgccccc gagggccaaa ccggcaaatc cgtgatcgcg ctcggcgtgc tggacaccct
     1741 gcttcgcgag gtcgagtcgg tcggcgtgtt ccgcccactg gtccgcgccg gcgagcccga
     1801 cgacattctc gagacgctgc tggcgcagcc cggcgtcgac cagtcctacg acgatgcggt
     1861 cggcatcacc tacgacgaga tgcgggccga cttcgattcg gccctggaaa ccatcgtcgg
     1921 caagttcggc cgcatcagtc cccgcttcga cgccatcgtc gtgctcggct cggactacgc
     1981 cgacgtgacc agcccgaccg aactcgcctt caacgctgag atcgcggcca acctgaacag
     2041 ttccctgctg ctcgtggtca gcggcttcga ccgcgatgcc cagcagatcc gggccgccgc
     2101 cgagtccgcg ctcaacgagt tcaccgccca ccacgtgcag accatcgggg tgatcgccaa
     2161 ccgcgtcgag ccctcgatcc tgaatgagac cgtcgccgag ctgcacgggc tgaccgacct
     2221 gctggtcgcc gcgctgccgg ccgagaaggt gctggtggcg cccaccctgc gggcccagta
     2281 cgaagccgtc ggcgcggtgc acgtgctggg caaccgcgac ctgatcgagc gcgagtcgca
     2341 agacgtggtg gtggccgcca tgaccctgcc gaacattctg tcgcggctgg tcaccgaggc
     2401 cacggtgctg gtgccgagcg accgctccga tctgctgccc gggctgattc tggcgcacca
     2461 ctcgggctcg ttcccccagc tcaccggcgt catcatgacc ggcggctacc cgattccgga
     2521 cgcggtggtg cggctgttcg acggcatgca tctcgacctg ccgatcggga tgaccgatct
     2581 cggcacgtac accacggcca agaaggtgtt caacacccag ggtcacttca ccgactcgcc
     2641 ccgcaaggtc gagatctcgc gccggctgtt ctccgagaac gtggacgccg acgcgctgat
     2701 ccgcgccatc gaggtcgagc cgtcccgcgc ggtcacgccg ctgatgttcg gctaccagct
     2761 caacgaactg gcccgccgcg accgccggcg catcgtgatg cccgagtcga ccgacgaccg
     2821 cattctcgag tcggcctcga tcctgctgca acggggcgtt gccgacctga ccctgctggg
     2881 cgacccggtc gagatcaaga cccgggcctc ggccatgggt tgggtgctgg acggggtgca
     2941 gatcgtcaat ccgaccgatc cggccctgct cgaccggttc agcaacgagt acgcgaaact
     3001 gcgcgcccac aagggcgtca ccgtcgggca ggcccgcgag aagatgaagg acctgagcta
     3061 cttcggcacc atgatggtgc acctcggcat ggccgacggc atggtgtcgg gcgcgatcaa
     3121 caccacggcg aacacgatcc ggcccagcct cgagttcatc aagaccaagc cgggcgtcaa
     3181 ggtggtgtcg ggctcgttcc tgatgtgcat gccggaccgg gtgcatgtgt acgccgactg
     3241 cgccgtcaac cccgacccga gcgccgagca gctggccgac atcgcgatca gttcggccga
     3301 aaccgccctc gccttcggca tcgaaccccg ggtggccatg ttgtcgtact cgaccggcac
     3361 ctcgggttcg ggtgcggacg tcgacaaggt tcggcaggcc accgacctgg tgcgcgagcg
     3421 ccgtccggac ctggctttgg aggggccgat ccagttcgac gccgcagtcg acccggcggt
     3481 cgcgaagacc aagctgcccg agtcggcggt ggccggccag gcgacggtat tcatctttcc
     3541 cgacctgaac acgggcaaca acacctacaa ggcggtgcag cggtcgtccg gcgcggtggc
     3601 gatcggcccg atcctgcagg gcctgaagaa ggcggtcaac gacctgtcgc ggggcgccct
     3661 ggtcgacgac atcgtcaaca ccgtggtgat caccgccatc caggcccagg ccgacgccgg
     3721 ctgagcaacg tccaccttca tccaacccac gccctaggag cgcccgcatg agcacgccca
     3781 tcctgctgtt gaacgccgga tcgtcctcga tcaagtacca ggtcatcgac gccgacaacg
     3841 aggacgtgct ggcgtccggc atcatccagc ggatcggtga cgaatccggc accatcgacc
     3901 accgggtcga cggccagacc caccaccaga cccgtggctt tcccaatcac gggcgggcgc
     3961 tgaccgtgct ggtgcagatg ttcgacgagt tcgggcccaa gctggccagc gtcaaggcgg
     4021 tcgggcatcg gatcgtgcac ggcggctcgc tgttcaactc gaccacggtg atcaacgacg
     4081 aggtgatcga gcatctgcgt gaggtgtcgc cgttggcacc cctgcacaac ccgccgggca
     4141 tcgccggcat cgaggcggcc atgcgcagcc tgccggacgt tccgcacgtc gcgatcttcg
     4201 ataccgcctt cttcagcacg cttcccgccg aggcgtacac ctacgcgatt ccccacgaac
     4261 tggccgctga gcacggcatt cgtaagtacg gctttcacgg cacgtcgcac agcttcgtca
     4321 gccgcaaggt cgccgaggtg ctcgacaagc cctacgactc gttcaaccag atcgtctgcc
     4381 acctcggcaa cggggcctcg atctcggcga tcgcgaacgg ggtcgcggtc gaggcgtcca
     4441 tgggtctcac cccgttggcc ggcctggtga tgggcacccg ctccggcgac gtcgaccccg
     4501 gcctgcacgc ctttctggcc cgccaggtgg gcatgagcat cgacgagatc gacaacaccc
     4561 tgaacaagaa gtcgggcatg ctcggcctct cgggggtcac cgactttcgt gacatcaacg
     4621 acctgatcgc cgagggtgac gcgggcgccg ttctggcgct cgacgtgtac gtgcaccggc
     4681 tgctcagcta catcggctcg tacatcgcga ttcttggcgg cgtcgaggcg atcaccttca
     4741 ccgccggggt cggcgagaac aaccccacgg tacgggccgc gatcgccacg cggctcgcct
     4801 tcctgggggt caggctggac gaggccgcga acgaggcccg ctcgaaggag ccgcgcgcga
     4861 tctcgacgcc ggagtcgtcc atcgcgatcc tcgtcgtgcc gacgaacgaa gagctcgcca
     4921 tggcgcgaga gacgaaggct gcgatcagct gacggatctg cggggcacgg ggtgcgggca
     4981 ccagggacgg acaggctgat ccgtcgccgg tcgagcctgt ccacaccctg gtgagcagcg
     5041 gttttgacat cgttcgaccg atggagggcg cgttggtccg cgcttgctac agcgcctcgg
     5101 acggatgcag cgcacgcagc ccgggcacca gcgcgaagtc ggagtcaagg gtgacgatgg
     5161 catcgaagcc cacctcgagg gcggtggccg cgtgcaccgc gtctcgtccg cgcagcggtg
     5221 tggcttcgac gagctggatc gcccgcagca gcacggcgcc ggtcaggtcg tgcaggtgca
     5281 gcacccccac cagtgcgcgg ctctgcgcga tcgccgactc gcgatcgctg acccgaagcc
     5341 catgaaagag gtactcctgg atcgcct
//
LOCUS       DAOSUG010000167         5355 bp    DNA     linear   ENV 10-JUN-2024
DEFINITION  MAG TPA_asm: Micropruina sp. isolate AMR_MDS_2195
            NODE_6887_length_5355_cov_4.799811, whole genome shotgun sequence.
ACCESSION   DAOSUG010000167 DAOSUG010000000
VERSION     DAOSUG010000167.1
DBLINK      BioProject: PRJNA925477
            BioSample: SAMN32864262
            Sequence Read Archive: SRR4236673
KEYWORDS    WGS; ENV; Metagenome Assembled Genome; MAG; Third Party Data; TPA;
            TPA:assembly.
SOURCE      Micropruina sp. (wastewater metagenome)
  ORGANISM  Micropruina sp.
            Bacteria; Bacillati; Actinomycetota; Actinomycetes;
            Propionibacteriales; Nocardioidaceae; Micropruina.
REFERENCE   1  (bases 1 to 5355)
  AUTHORS   Abdulkadir,N., Saraiva,J.P., Zhang,J., Stolte,S., Gillor,O.,
            Harms,H. and Rocha,U.
  TITLE     Genome-centric analyses of 165 metagenomes show that mobile genetic
            elements are crucial for the transmission of antimicrobial
            resistance genes to pathogens in activated sludge and wastewater
  JOURNAL   Microbiol Spectr 12 (3), e0291823 (2024)
   PUBMED   38289113
REFERENCE   2  (bases 1 to 5355)
  AUTHORS   Abdulkadir,N.
  TITLE     Direct Submission
  JOURNAL   Submitted (24-MAR-2023) Environmental Microbiology, Helmholtz
            Center for Environmental Research -UFZ, Permoserstr 15, Leipzig
            04318, Germany
COMMENT     The annotation was added by the NCBI Prokaryotic Genome Annotation
            Pipeline (PGAP). Information about PGAP can be found here:
            https://www.ncbi.nlm.nih.gov/genome/annotation_prok/
            
            ##Genome-Assembly-Data-START##
            Assembly Method        :: SPAdes v. 3.11.1
            Genome Representation  :: Full
            Expected Final Version :: Yes
            Genome Coverage        :: Not Applicable
            Sequencing Technology  :: Illumina MiSeq
            ##Genome-Assembly-Data-END##
            
            ##Genome-Annotation-Data-START##
            Annotation Provider               :: NCBI
            Annotation Date                   :: 05/04/2023 04:57:10
            Annotation Pipeline               :: NCBI Prokaryotic Genome
                                                 Annotation Pipeline (PGAP)
            Annotation Method                 :: Best-placed reference protein
                                                 set; GeneMarkS-2+
            Annotation Software revision      :: 6.5
            Features Annotated                :: Gene; CDS; rRNA; tRNA; ncRNA
            Genes (total)                     :: 2,776
            CDSs (total)                      :: 2,750
            Genes (coding)                    :: 2,714
            CDSs (with protein)               :: 2,714
            Genes (RNA)                       :: 26
            tRNAs                             :: 24
            ncRNAs                            :: 2
            Pseudo Genes (total)              :: 36
            CDSs (without protein)            :: 36
            Pseudo Genes (ambiguous residues) :: 0 of 36
            Pseudo Genes (frameshifted)       :: 2 of 36
            Pseudo Genes (incomplete)         :: 32 of 36
            Pseudo Genes (internal stop)      :: 2 of 36
            ##Genome-Annotation-Data-END##
FEATURES             Location/Qualifiers
     source          1..5355
                     /organism="Micropruina sp."
                     /mol_type="genomic DNA"
                     /submitter_seqid="NODE_6887_length_5355_cov_4.799811"
                     /isolate="AMR_MDS_2195"
                     /isolation_source="Activated sludge of municipal
                     wastewater treatment plants"
                     /db_xref="taxon:2737536"
                     /environmental_sample
                     /geo_loc_name="Canada"
                     /lat_lon="49.25 N 123.1 W"
                     /collection_date="2015-06-16"
                     /metagenome_source="wastewater metagenome"
                     /note="metagenomic"
     gene            258..764
                     /locus_tag="PLF56_06715"
     CDS             258..764
                     /locus_tag="PLF56_06715"
                     /inference="COORDINATES: protein motif:HMM:NF024854.2"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_function: GO:0003700 - DNA-binding transcription factor
                     activity [Evidence IEA];
                     GO_process: GO:0006355 - regulation of DNA-templated
                     transcription [Evidence IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="MarR family winged helix-turn-helix
                     transcriptional regulator"
                     /protein_id="HOQ53291.1"
                     /translation="MSLGEALDVDVGAASRAELAAQLLTLNGQVHDRAMGLVGPMAVP
                     ADLTLQQLRVLDHVAKQPGLSGQELGALLGVSAPTASGLVERLVEKGLVVRVDDRDDR
                     RVRRLQPTEAGLDVMRQMDSVFERCLAVVLEHLSIEDLRLLCRGARAMLSALERVRDD
                     RSEPSSLR"
     gene            complement(940..2058)
                     /locus_tag="PLF56_06720"
     CDS             complement(940..2058)
                     /locus_tag="PLF56_06720"
                     /inference="COORDINATES: protein motif:HMM:NF015651.2"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="lysylphosphatidylglycerol synthase
                     domain-containing protein"
                     /protein_id="HOQ53292.1"
                     /translation="MTEPAHPVPPSGSAEAEPVPSPRELLAEVPRRELKAAWVGRLIT
                     LAVAVLLVIVIGRFIRRVDWPSVGAALAQLSWWQLLLLFVLVLLRQVANGAPLMFNIP
                     GVTLRQAAVSDLAASTMGVIAPPPSESALRIAMFRSWGTPMSLAVAGAVMNSVTFFLI
                     RFSAPAFGFVVVALTGRPLGLRWLDVISLLIAAVLLVGVLLVVRTATWARWVGLQAGR
                     LTRRIRPSVDPEAWAQACASFRDSIAGRFGYAFPRALLANLVMLMLDATILFASLRFV
                     GLTPAQLPAADILIAYLFAFPLTAFPMSGLGVVDAAALASMIEAGGAGVTEPAIAGLI
                     IWRVFTVVVVFLLGLIAVAAWRRTSGRTPSAHDIAAGA"
     gene            complement(2229..2405)
                     /locus_tag="PLF56_06725"
     CDS             complement(2229..2405)
                     /locus_tag="PLF56_06725"
                     /inference="COORDINATES: ab initio
                     prediction:GeneMarkS-2+"
                     /note="Derived by automated computational analysis using
                     gene prediction method: GeneMarkS-2+."
                     /codon_start=1
                     /transl_table=11
                     /product="hypothetical protein"
                     /protein_id="HOQ53293.1"
                     /translation="MSSFRHDARAALVGLAAGLVIARMLTDPAWASPSPSWTHMPHTR
                     AQDVLKAEQHPPEA"
     gene            complement(2516..2914)
                     /locus_tag="PLF56_06730"
     CDS             complement(2516..2914)
                     /locus_tag="PLF56_06730"
                     /inference="COORDINATES: ab initio
                     prediction:GeneMarkS-2+"
                     /note="Derived by automated computational analysis using
                     gene prediction method: GeneMarkS-2+."
                     /codon_start=1
                     /transl_table=11
                     /product="type II toxin-antitoxin system VapC family
                     toxin"
                     /protein_id="HOQ53294.1"
                     /translation="MRIVLDTDVSSLLLKRTLPPELLAQLAPHEVAITFVTVGELRKW
                     AVVRQLGARRRAELDSWVDARPKIPAGADVARTWGEIVGYAEHRGRPRPVNDSWIAAC
                     CLTYDLPLATLNTADFHDYATYEGLQLLAP"
     gene            complement(2921..3154)
                     /locus_tag="PLF56_06735"
     CDS             complement(2921..3154)
                     /locus_tag="PLF56_06735"
                     /inference="COORDINATES: ab initio
                     prediction:GeneMarkS-2+"
                     /note="Derived by automated computational analysis using
                     gene prediction method: GeneMarkS-2+."
                     /codon_start=1
                     /transl_table=11
                     /product="hypothetical protein"
                     /protein_id="HOQ53295.1"
                     /translation="MPHLEHEVDEEVITVSTNPQPIPALSSSDRVDLDELFRDAGPVG
                     DGSDLAAPGVFETPEELDEFLAWLRADRAAGLA"
     gene            complement(3623..4966)
                     /locus_tag="PLF56_06740"
     CDS             complement(3623..4966)
                     /locus_tag="PLF56_06740"
                     /inference="COORDINATES: protein motif:HMM:NF018448.2"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="threonine/serine exporter family protein"
                     /protein_id="HOQ53296.1"
                     /translation="MTEGVHSTPAAIALAEKSDAVCRMGALMLGAGTGSYRVKAAMGR
                     VAAALGIEELEAQVSLNEIVATARAEGTFRTQVVEASPPVVNADRIRALLRVSLRAEP
                     GVRASDLQRQLDRVEQRAALYPFAVVVAGAAVACAAFAFLNNGRWQECVAAGVAAALG
                     KAVQVSVHRRLRLNQLATIALASTIACVSYLLLADLVRTLGGGASPLQPGAFTSAILF
                     LVPGFPLITAALDLARFDFTSGMSRLLYATMITLAAAVGASIAALTFGLTPGELPPPV
                     ASDAVLLVLRVVASFLGILGFAVTFNTPWRIALAASAIGCIANVPRLFALDHGWNALL
                     CATLAACAIGLMAGWASQRIAAPRIILSVPAVLIMIPGASTYRAMVAIINRDPLSTLE
                     QGTASLGVLLGLAAGLVIARMLTDPAWASPSPSWTHMPHTRAQDVLKAEQHPPEA"
     gene            complement(4963..>5355)
                     /locus_tag="PLF56_06745"
     CDS             complement(4963..>5355)
                     /locus_tag="PLF56_06745"
                     /inference="COORDINATES: ab initio
                     prediction:GeneMarkS-2+"
                     /note="Derived by automated computational analysis using
                     gene prediction method: GeneMarkS-2+."
                     /codon_start=1
                     /transl_table=11
                     /product="hypothetical protein"
                     /protein_id="HOQ53297.1"
                     /translation="PAERSWGDQPELILQPIVHVADAPGTVRLLEALGGEVVQGSRSG
                     DWVQVEIGGAQVGLLAHPPAVELGEGVVELAFGSRTPLAEVESRAREQGVAVAAPSGD
                     EGFGRQLRLRSPDGLVVTINEIDAELIE"
BASE COUNT          896 a         1829 c         1862 g          768 t
ORIGIN      
        1 cgctgaccac gttgccgcgc ggcacgtcga tcgaggggat gagttcctgt ttgagtgcgg
       61 tcgtggcata gaggcccata ccgatggtga gcagcgtgag cagcagcacc accgtgcgat
      121 gcgcaaggct gagtttggtc aggcgaagca ctgtttcccc ttcatcggcc cctcacggtt
      181 gggggcaacg ccatggcccg ggccgaccac atcggttagt gtagccctaa ctattaggcc
      241 gaccgaaagt gagtcacatg tcgttggggg aggcactgga cgtcgacgtc ggtgcggcgt
      301 cgcgggccga actggccgcg caactgctga ccctcaacgg gcaggtgcac gaccgtgcga
      361 tgggcctggt ggggccgatg gcggtacccg ccgacctcac ccttcaacaa ctgcgggtgc
      421 tcgatcacgt ggcgaagcaa cccgggctgt ccggccagga actcggcgcg ctgctgggcg
      481 tcagcgcacc gaccgccagc ggactggtcg agcggctggt cgaaaaaggg ctggtcgtgc
      541 gcgtcgacga tcgggacgac cggcgcgtgc gccggctgca accgaccgag gccggcctcg
      601 atgtgatgcg gcaaatggac tcggtgttcg aacgttgcct ggcggtggtg ctggagcacc
      661 tgtcgatcga ggatctgcgc ctgttgtgcc ggggtgccag ggcaatgctg agcgccctgg
      721 agcgcgtccg ggacgatcga tcggagccgt cgtccctgcg atgagcagca tgtgggaacg
      781 gcgttgccat tcctgctgga cggctgtgaa ccgcgttggg gacggtgctc cactgcgttc
      841 accgcatcga ccgggctgtg aatcgagtgg gggacacccc gtccacgtgc ctgatcgctg
      901 gggtggaggg ctcaccccca ccccccgatc cacgcctatt caggctcccg cagcgatgtc
      961 gtgcgcgctc ggcgtccgtc cgctggtacg gcgccacgcg gccaccgcga tcaagccgag
     1021 cagaaacacc accaccaccg tgaacacccg ccagatgatc aggcccgcga tcgccggctc
     1081 ggtcaccccg gcgccacccg cctcgatcat ggacgcgagc gctgccgcgt ccaccacccc
     1141 gagccccgac atggggaacg ccgtcaacgg aaaggcgaac aggtaggcga tcagaatgtc
     1201 cgcggccggc agttgggccg gggtcaaccc gacgaaacgc agcgaggcga acaagatggt
     1261 ggcgtccagc atcagcatca ccagattggc cagcagggca cggggaaacg cgtagccgaa
     1321 gcgtccggcg atgctgtcgc ggaacgaggc gcaggcctgc gcccacgcct ccggatcgac
     1381 gctggggcgg attcgacggg tcaggcgtcc ggcctgcagt cccacccagc gcgcccacgt
     1441 cgcggtgcgc accaccagca acaccccgac cagcaggacg gccgcgatca gcaggctgat
     1501 cacgtcgagc caacgcaggc ccagcggacg tccggtcagt gcgacgacaa cgaacccgaa
     1561 cgccggagcc gagaaccgga tcagaaagaa cgtgacggag ttcatcaccg cgccggcgac
     1621 ggcgagcgac atgggcgtgc cccaggaccg gaacatggcg atccgcaggg cggattccga
     1681 cgggggcggt gcgatcaccc ccatcgtcga ggcggccagg tcgctgaccg ctgcctggcg
     1741 cagggtgaca ccgggaatgt tgaacatcaa cggggcgccg ttcgcgacct gacgcagcag
     1801 caccagaacg aacagcagca gcagttgcca ccatgacagc tgagccagcg cggccccgac
     1861 gctcggccag tccacccgac gaatgaagcg gccgatcacg atcaccagca gcacggccac
     1921 cgccagggtg atcagcctgc ccacccaggc ggccttgagt tcgcgcctcg gcacctcggc
     1981 aagcagctcg cgcggggacg gcaccggttc ggcctctgct gagccggatg gggggacggg
     2041 gtgtgctggc tcagtcatgg tgcggctcat tatgccgccg gcccgaacac ccccctaact
     2101 gtccagcgcc tgcgcaactc cgcggagttg cgcaggcgct ggacagttag gcgggtgttg
     2161 gcgccgctgg cctcgtgcag cccgggactc ggatgactcg actgcggcga ccgccgccca
     2221 tagctcgatc acgcctcggg tggatgttgc tccgccttca ggacgtcctg ggcccgggtg
     2281 tgcggcatgt gcgtccagct ggggctcggc gaggcccagg ccgggtcggt gagcatgcgt
     2341 gcgatcacca ggccggccgc gagtccgacc aacgcagccc tcgcgtcatg cctaaaggat
     2401 gacaccgccg acgaaggtgc cagagatcca gaccggggag ggctccgatg tgcgccgggt
     2461 gtatcgcggt tggatctgcg cgtgtctggg gcatgaatgt ggtcagggtt caccgtcaag
     2521 gggcgaggag ctggagacct tcgtaggtgg cgtagtcgtg gaagtcggcg gtgttgaggg
     2581 tggcgagggg aaggtcgtag gtcaggcagc aggcggcgat ccaggagtcg ttcactggtc
     2641 gcgggcggcc gcggtgctcg gcgtagccga cgatctcacc ccacgtgcga gcgacgtcgg
     2701 cgccggccgg tatcttcggc cgtgcgtcca cccacgagtc cagttctgct cgacgccgcg
     2761 ccccgagctg gcgtacgacg gcccacttgc gcaactcgcc gaccgtgacg aaggtgatgg
     2821 ccacctcgtg cggggccagc tgcgccaaga gttccggcgg cagtgtgcgc ttgagtagca
     2881 gcgacgacac gtcggtgtcg agaacgatcc gcatcaggtg tcaggcgagt ccggcggccc
     2941 ggtccgcgcg tagccaggca agaaactcgt cgagttcctc cggtgtttcg aacacgcccg
     3001 gcgctgcgag atcgctgcca tcgccgaccg gccccgcgtc acggaacagc tcgtccaagt
     3061 cgacacggtc gctgctgctg agagcaggta tcggctgcgg attcgtggac accgtgatca
     3121 cctcctcgtc gacctcgtgc tccaagtgtg gcacgggggc gacattttgc gagcgtccgg
     3181 ggagtgccgt tggggcgcgc gggcccgatg ggctaggcct cggcggtcag gctcggccta
     3241 tcgcgctcag ttccggctac ttcctcgctt cgcgccgcgt cgctctgtgt ttggccacac
     3301 actcgtcggc gtcgaccccg cgaccgacaa gcagtccgtc cagggcgtcg gcggccgcgc
     3361 ggagcgcggc gatctcggga tctcgacacc cagggtgtag cgatctacaa gaggatgtgg
     3421 ggacttctct agcgattcaa tgcagggcgg ccagaactcc gacgccgaca cccggcccga
     3481 acatccccct aactgtccaa cgcctgcgca actccgcgga gttgcgcagg cgctggacag
     3541 ttaggcgggt gttggcgccg ctggcctcgt gcagcccggg actcggatga ctcgactgcg
     3601 gcgaccgccg cccatagctc gatcacgcct cgggtggatg ttgctccgcc ttcaggacgt
     3661 cctgggcccg ggtgtgcggc atgtgcgtcc agctggggct cggcgaggcc caggccgggt
     3721 cggtgagcat gcgtgcgatc accaggccgg ccgcgagtcc gagcaacact ccgaggctcg
     3781 ccgtgccctg ctcgagcgtg ctgagggggt cacggttgat gatcgccacc atggcccggt
     3841 aggtgctggc gccgggaatc atgatcagca ccgcaggcac gctcagaatg atccgcgggg
     3901 cggcgattcg ttgactggcc cacccggcca tcaggccgat cgcgcacgcg gccagggtgg
     3961 cgcagagcag cgcgttccag ccgtgatcca gcgcgaacag ccggggaacg ttggcgatgc
     4021 aaccgatcgc cgacgcggcc agtgcgatcc gccacggcgt gttgaacgtc accgcgaagc
     4081 cgagaatgcc gaggaagctc gccaccacgc gcagcaccag cagcacggcg tccgaagcga
     4141 cgggcggcgg cagttcgcct ggcgtcagtc cgaacgtgag cgccgcgatc gaagcgccga
     4201 cggcagcggc cagcgtgatc atggtggcgt agagcagccg tgacatgccc gaggtgaagt
     4261 cgaaccgggc cagatcaagc gcggccgtga tcagcggaaa gccaggcacc agaaagagga
     4321 tcgccgaggt gaacgccccc ggctgcagcg ggctcgcccc accgcccagc gtgcgcacca
     4381 ggtcggccag cagcaggtag ctgacgcagg cgatcgtcga cgccagcgcg atcgtggcca
     4441 actgattgag ccgcagccgg cgatgcacgc tgacctgcac cgccttgccg agtgcggcgg
     4501 ccaccccggc cgccacgcat tcctgccagc gtccgttgtt gagaaaggcg aacgcggcgc
     4561 acgcgaccgc tgccccggcg accaccaccg cgaacgggta cagcgcggcc cgctgctcga
     4621 cccggtcgag ttggcgctgc agatcggagg cccgcacgcc cggctccgcg cgcaatgaaa
     4681 cgcgcagcag ggcacggatc cggtccgcgt tgaccaccgg cggtgaggcc tcgaccacct
     4741 gggtgcggaa cgtgccctcg gcccgcgcgg tcgcgacgat ctcgttgagg ctgacctgag
     4801 cctccaactc ctcgatgccc agggcggccg ccacccgtcc catcgcggcc ttgacccggt
     4861 acgagccggt gccggcgccc agcatcaggg cgcccatgcg gcagaccgcg tccgacttct
     4921 cggccagcgc gatggcggcc ggcgtggagt ggacgccctc agtcactcga tcagttcagc
     4981 atcgatctcg ttgatggtca ccaccagtcc atcgggcgag cgcagtcgca actgtcgacc
     5041 gaaaccctca tcgccgctcg gcgcggccac ggcgaccccc tgttcacgcg ctcgcgactc
     5101 gacctcggca aggggagtgc gcgacccgaa cgccagttcc accacgccct caccgagttc
     5161 gaccgcgggc gggtgggcga gcaggcccac ctgtgcaccg ccgatctcga cctgcaccca
     5221 gtcgcctgat cggctgccct ggaccacctc gccacccaat gcctccagca gccggacggt
     5281 gccgggcgcg tccgcgacat gcacgatcgg ctgcaggatc agttccggtt ggtcacccca
     5341 gctgcgctcg gcggg
//
LOCUS       DAOSUG010000168         5355 bp    DNA     linear   ENV 10-JUN-2024
DEFINITION  MAG TPA_asm: Micropruina sp. isolate AMR_MDS_2195
            NODE_6888_length_5355_cov_4.729811, whole genome shotgun sequence.
ACCESSION   DAOSUG010000168 DAOSUG010000000
VERSION     DAOSUG010000168.1
DBLINK      BioProject: PRJNA925477
            BioSample: SAMN32864262
            Sequence Read Archive: SRR4236673
KEYWORDS    WGS; ENV; Metagenome Assembled Genome; MAG; Third Party Data; TPA;
            TPA:assembly.
SOURCE      Micropruina sp. (wastewater metagenome)
  ORGANISM  Micropruina sp.
            Bacteria; Bacillati; Actinomycetota; Actinomycetes;
            Propionibacteriales; Nocardioidaceae; Micropruina.
REFERENCE   1  (bases 1 to 5355)
  AUTHORS   Abdulkadir,N., Saraiva,J.P., Zhang,J., Stolte,S., Gillor,O.,
            Harms,H. and Rocha,U.
  TITLE     Genome-centric analyses of 165 metagenomes show that mobile genetic
            elements are crucial for the transmission of antimicrobial
            resistance genes to pathogens in activated sludge and wastewater
  JOURNAL   Microbiol Spectr 12 (3), e0291823 (2024)
   PUBMED   38289113
REFERENCE   2  (bases 1 to 5355)
  AUTHORS   Abdulkadir,N.
  TITLE     Direct Submission
  JOURNAL   Submitted (24-MAR-2023) Environmental Microbiology, Helmholtz
            Center for Environmental Research -UFZ, Permoserstr 15, Leipzig
            04318, Germany
COMMENT     The annotation was added by the NCBI Prokaryotic Genome Annotation
            Pipeline (PGAP). Information about PGAP can be found here:
            https://www.ncbi.nlm.nih.gov/genome/annotation_prok/
            
            ##Genome-Assembly-Data-START##
            Assembly Method        :: SPAdes v. 3.11.1
            Genome Representation  :: Full
            Expected Final Version :: Yes
            Genome Coverage        :: Not Applicable
            Sequencing Technology  :: Illumina MiSeq
            ##Genome-Assembly-Data-END##
            
            ##Genome-Annotation-Data-START##
            Annotation Provider               :: NCBI
            Annotation Date                   :: 05/04/2023 04:57:10
            Annotation Pipeline               :: NCBI Prokaryotic Genome
                                                 Annotation Pipeline (PGAP)
            Annotation Method                 :: Best-placed reference protein
                                                 set; GeneMarkS-2+
            Annotation Software revision      :: 6.5
            Features Annotated                :: Gene; CDS; rRNA; tRNA; ncRNA
            Genes (total)                     :: 2,776
            CDSs (total)                      :: 2,750
            Genes (coding)                    :: 2,714
            CDSs (with protein)               :: 2,714
            Genes (RNA)                       :: 26
            tRNAs                             :: 24
            ncRNAs                            :: 2
            Pseudo Genes (total)              :: 36
            CDSs (without protein)            :: 36
            Pseudo Genes (ambiguous residues) :: 0 of 36
            Pseudo Genes (frameshifted)       :: 2 of 36
            Pseudo Genes (incomplete)         :: 32 of 36
            Pseudo Genes (internal stop)      :: 2 of 36
            ##Genome-Annotation-Data-END##
FEATURES             Location/Qualifiers
     source          1..5355
                     /organism="Micropruina sp."
                     /mol_type="genomic DNA"
                     /submitter_seqid="NODE_6888_length_5355_cov_4.729811"
                     /isolate="AMR_MDS_2195"
                     /isolation_source="Activated sludge of municipal
                     wastewater treatment plants"
                     /db_xref="taxon:2737536"
                     /environmental_sample
                     /geo_loc_name="Canada"
                     /lat_lon="49.25 N 123.1 W"
                     /collection_date="2015-06-16"
                     /metagenome_source="wastewater metagenome"
                     /note="metagenomic"
     gene            215..1183
                     /gene="galE"
                     /locus_tag="PLF56_06750"
     CDS             215..1183
                     /gene="galE"
                     /locus_tag="PLF56_06750"
                     /EC_number="5.1.3.2"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_013160189.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_function: GO:0003978 - UDP-glucose 4-epimerase activity
                     [Evidence IEA];
                     GO_process: GO:0006012 - galactose metabolic process
                     [Evidence IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="UDP-glucose 4-epimerase GalE"
                     /protein_id="HOQ53298.1"
                     /translation="MSTWLVTGGAGYIGAHVVRAFSSVGITPVVVDDLSTGRAAFVPP
                     EVTLVQGDILDVDLLTDTLDRFNCTGVVHVAGFKYAGVSVKRPLHTYTQNVTGMVKVL
                     DAMHRAGVNKLVFSSSAAVYGTPSTELVTEDSPTHPESPYGESKLVGEWLMSDVARAW
                     PEFSSVALRYFNVVGSATDEVYDISPHNLFPIVIEALIEGRTPQVNGTDYATPDGSCV
                     RDYVHVGDLAISHVAAAQALEAGRELLPAYNLGSGEGLSVLQIMDAMRRVTGVDFTPD
                     VGPRRAGDPARIVADGTAAAADLDWHMRHTVDEMVASAWSARRAHG"
     gene            complement(1356..2465)
                     /locus_tag="PLF56_06755"
     CDS             complement(1356..2465)
                     /locus_tag="PLF56_06755"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_013863438.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_function: GO:0004803 - transposase activity [Evidence
                     IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="IS630 family transposase"
                     /protein_id="HOQ53299.1"
                     /translation="MPKAPAAPLMVSAEQRAELAAIARSTTRPHRVVVQARGLLWAAE
                     GVANAEIARRCEVDADAVRRWRTRFATAGVAGVGIIAKGRGRKPSLPAGTVEEVLRLT
                     REETPPDTSTHWSTRSMAARVGIGKDAVAKIWADNNLKPWQVTTFKISNDPCFEEKLV
                     DVVGLYLDPPARAVVFSYDEKTQCQALDRTQPSLPMKPGRAGTMTHDYKRNGTIDLFA
                     AMNLATGQVLTGLNKGHTGADILQFFKQIDASVPRELDVHVVLDNLSAHSTPDIKKWL
                     AHPDRQRWHLHFTPTSSSWLNLIERWFKELTDRRLRRGTFTSVQDLSHAITTWAEHWN
                     ADPKPFIWKATAQDIITKVQRGRDTLHQTNSQTDH"
     gene            complement(2484..3974)
                     /locus_tag="PLF56_06760"
     CDS             complement(2484..3974)
                     /locus_tag="PLF56_06760"
                     /inference="COORDINATES: protein motif:HMM:NF015648.2"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="PH domain-containing protein"
                     /protein_id="HOQ53300.1"
                     /translation="MSIPPGSFSGPVQPQTIGSGEPGPAENRDDRAWPHAGSSNVPVG
                     TESVRALVEKPHPLTPLVRAWVLVLAVAWTIGRDLLGRPEGLRLPPLTWVTGGVGAVL
                     VTLLVAAYLDWRATAFVVDHGELRLERGVFTKSSERIRFDRIQSVDITEPFAARLLGL
                     AEVTIDVGAEGGHRMRYLSRARAAAMRDYLLARAHGVRPAEVTRQSTTGVLDDLAAGD
                     EVLVRVPSQRLVVGALLSHEFLLLTVPVGLTIATVIALDPRGSNAALSNPWLLLGAAL
                     PALGGLWSFVMRRVIGQWHYAFVRSGPGLKITRGLTSLSSQSVPRHRIQSLRIAQPLW
                     WRRLGYYRVDMSVLGNHGLTTDEDQAGRTSILLPIGTRAEVDLVLNTIWPGLNLDGIA
                     IEHSPERARWVQPLAQGWVGWGSDGSVLVTRMGWLTRTQHVVPHARVQSVALQQGPLL
                     RRLRLASVAVHTTQALAVNAALHLDAYTARALLFAESRRASGPSVK"
     gene            complement(3971..4459)
                     /locus_tag="PLF56_06765"
     CDS             complement(3971..4459)
                     /locus_tag="PLF56_06765"
                     /inference="COORDINATES: protein motif:HMM:NF015648.2"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_function: GO:0005515 - protein binding [Evidence IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="PH domain-containing protein"
                     /protein_id="HOQ53301.1"
                     /translation="MDDLFAPPAVGWRRISPAYAMVLRARMLIGTALVIGVVATVLWH
                     AVDPRWAGALIALGVGYAGWQWFRLGRWVRAWGYLERDDDLYLSHGLWSRELIVIPYG
                     RMQAVKVESGPLTRALGLANVELVTASLQSGGRVPGLPQRDAEALRDRLIAIGIGDQD
                     GL"
     gene            complement(4524..5156)
                     /locus_tag="PLF56_06770"
     CDS             complement(4524..5156)
                     /locus_tag="PLF56_06770"
                     /inference="COORDINATES: protein motif:HMM:NF021443.2"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="DUF1707 domain-containing protein"
                     /protein_id="HOQ53302.1"
                     /translation="MSYAEPTDVGPAYGGDLAVNASDKTKVLTLLDAALAEGRITPAE
                     HAERTQAANQAQTFDDLVPLTRDLVVVPGPAATPTWSSAPVGPDTGPEMIVAIFSGAA
                     RKGVWQPRRNLSVLTLFGGAELDLTQAVFTDNVCEINVFCLFGGIEITVPDGTTVRNE
                     CMAVFGGSETKTMPPTPGAPTVIVKGFIGFGGVEVRGLSKKQRKRRDCGS"
     gene            5300..5355
                     /locus_tag="PLF56_06775"
     tRNA            5300..5355
                     /locus_tag="PLF56_06775"
                     /product="tRNA-Gly"
                     /inference="COORDINATES: profile:tRNAscan-SE:2.0.12"
                     /note="Derived by automated computational analysis using
                     gene prediction method: tRNAscan-SE."
                     /anticodon=(pos:5324..5326,aa:Gly,seq:ccc)
BASE COUNT          833 a         1787 c         1890 g          845 t
ORIGIN      
        1 catcctgaca tgaggcaacg acccccacct acgcgggcac cttgacatga ggcatcaggg
       61 tgttggcctg cgtcacggac ggctgcttcg cccggctcct gtgactcagc ggccgttgac
      121 gtctgtggca cgctgcgggc aggaactgcg cagaggttca ggctcaccgg tgttgcgagt
      181 ggcgggcggc cgtggctgcc gacagactgg gggggtgagt acctggttgg tcaccggcgg
      241 cgccggctac atcggcgcgc acgtggtgcg cgcgttcagt tccgtgggca tcacgcccgt
      301 ggtggtcgac gatctgtcga ccggacgggc ggccttcgtg ccgcccgagg tcaccttggt
      361 gcagggcgac attctcgacg tcgacctgct caccgacacc ctcgatcggt tcaactgcac
      421 cggcgtggtg cacgtcgccg gcttcaagta cgcgggcgtg tcggtcaagc ggccgctgca
      481 cacctacacc cagaacgtca cgggcatggt gaaggtgctc gacgccatgc accgggccgg
      541 cgtgaacaag ctggtgttca gctccagcgc ggcggtgtac ggcacgccgt ccaccgaact
      601 ggtcaccgaa gactccccga cccaccccga atcgccctac ggcgagagca agctggtggg
      661 cgagtggctg atgagtgacg tggcacgggc ctggcccgag ttctcgagcg tcgccctgcg
      721 ctacttcaac gtggtcggct cggccaccga cgaggtgtac gacatcagtc cgcacaacct
      781 gtttccgatc gtcatcgagg cgctgatcga gggccggaca ccgcaggtga acggcaccga
      841 ctacgccacg cccgacggct cgtgcgtacg cgactacgtg cacgtgggcg acctcgcgat
      901 ctcgcacgtg gcggccgccc aggcgctcga ggccggtcgc gaactgctgc ccgcctacaa
      961 cctgggcagc ggggaagggc tcagcgtgct gcagatcatg gacgccatgc gccgcgtcac
     1021 cggtgtcgac ttcacgcccg acgtgggtcc gcgtcgtgcg ggtgatcctg cccgaatcgt
     1081 cgcggacggc accgcggcgg ccgccgacct cgactggcat atgcggcaca ccgtcgacga
     1141 gatggtcgcc agcgcgtgga gcgcccgccg cgcacacggc tgaggcgcag cgtcagccca
     1201 gcggcgtcgc tgtggtggcg tcctgagatc ccggggcaag cccgggatga cggtggacgc
     1261 ctttctcgtc atcccggcgg acgccgggat ctcaggtgca ggcagcgcgt tgccctcaag
     1321 cggcgctgtc gaacaatgcg tcgagccgca cgtccctagt ggtccgtctg tgaattggtc
     1381 tggtggaggg tgtcgcgacc tcgttggacc ttggtgatga tgtcttgagc ggtggctttc
     1441 cagatgaagg gtttggggtc ggcgttccag tgttcagccc aggtggtgat ggcgtgggag
     1501 aggtcttgga cgctggtgaa ggtgccgcgt cggaggcgtc tgtcggtgag ttctttgaac
     1561 catcgctcga tcaggttcag ccaggagctg gaggtggggg tgaagtgcag gtgccagcgt
     1621 tgccgatcgg ggtgggcgag ccatttcttg atgtcgggag tggagtgcgc cgacaggttg
     1681 tccaagacga cgtgcacgtc gagttcgcgc ggcacgctgg catcgatctg tttgaagaac
     1741 tgcaggatgt cggctccggt gtggcccttg ttgaggccgg tcaggacctg accggtggcg
     1801 aggttcatgg cggcgaacag gtcgatggtg ccgttgcgtt tgtagtcgtg ggtcatggtg
     1861 ccggcccggc cgggtttcat cggtagcgag ggttgggtgc ggtccagcgc ttggcattgg
     1921 gttttctcgt cgtaggagaa gaccactgct cgtgctggcg ggtccaggta gagcccgacg
     1981 acatcgacca gtttctcctc gaagcaggga tcgttgctga tcttgaaagt ggtcacctgc
     2041 cagggtttga ggttgttgtc ggcccagatc ttcgcgaccg cgtccttacc gatcccgacg
     2101 cgggcggcca tggaccgggt cgaccagtgg gtcgaggtgt ctggtggcgt ttcctcgcgg
     2161 gtcaggcgca gcacctcttc gaccgttccc gcaggcagcg acggtttccg gccacggccc
     2221 ttcgcgatga tcccgacccc cgcgacacca gccgtggcga accgggtccg ccaccgcctc
     2281 accgcgtcgg catcgacctc acaacggcgg gcgatctccg cgttcgcgac accctcggcg
     2341 gcccacagca gcccccgggc ctggaccacg acccgatgcg ggcgggtcgt ggaccgcgct
     2401 atcgcggcca actccgcgcg ttgctcagca gagaccatca agggtgcggc aggcgctttc
     2461 ggcataccat cagaataccc ttgctatttc acagacggac cactagcgcg ccgagactcc
     2521 gcgaacagca gtgcgcgggc ggtgtaagcg tccaggtgta gcgccgcgtt gacggcgagg
     2581 gcctgggtgg tgtgcacggc gaccgaggcc agccgcagcc ggcgcaacaa cggcccctgc
     2641 tgcagcgcca ccgactgaac ccgcgcgtgc ggcacgacgt gttgggtgcg ggtcagccag
     2701 cccatgcggg tgaccagcac cgagccgtcc gaaccccagc cgacccagcc ctgggccagc
     2761 ggctgcaccc agcgagcccg ctcggggctg tgttcgatgg cgatgccgtc gaggttgagg
     2821 ccgggccaga tggtgttcag caccaggtcg acctctgctc gggtaccgat gggcagcaga
     2881 atgctcgtgc gcccggcctg gtcttcgtcg gtggtgagcc cgtggttacc gagcaccgac
     2941 atgtcgacgc ggtaatagcc gagccgacgc caccacagcg gctgggcgat gcgcaacgac
     3001 tgaatgcggt ggcggggcac cgactggctg ctcaggctgg tcaggccgcg ggtgatcttg
     3061 agcccggggc cgctgcgcac gaacgcgtag tgccactggc cgatcacccg ccgcatgacg
     3121 aagctccaca agccgcccag cgccggcaga gcagccccga gcagcagcca cggattgctc
     3181 aatgcggcgt tcgaaccgcg cggatcgagg gcgatcaccg tggcgatcgt gagccccacc
     3241 ggcaccgtca acagtagaaa ctcgtgcgag agcagggcgc ccaccaccag gcgttgcgac
     3301 ggtacgcgca ccagcacctc gtcgccggcg gccagatcgt ccagcacacc ggtggtgctc
     3361 tgccgagtga cctcggccgg ccgtactccg tgggcccggg ccagcaggta gtcgcgcatg
     3421 gcggcggccc gcgcccgtga caggtaccgc atgcgatgac cgccctcggc gcccacgtcg
     3481 atggtcacct cggcgagccc cagcaagcgc gcggcgaacg gttcggtgat gtcgaccgac
     3541 tgaatgcggt cgaacctgat gcgttcggac gacttggtga acacgccccg ctcgagtcgt
     3601 agctcgccgt gatcgaccac gaacgcggtg gcccgccagt cgaggtaggc cgccaccagc
     3661 agcgtcacca gtaccgcgcc gacaccaccg gtcacccagg tcagcggcgg caggcgcagc
     3721 ccctcgggcc gacccagcag gtcgcggccg atcgtccagg ccacggccag caccagcacc
     3781 caggcccgca ccagcggggt gagcgggtgg ggcttctcga ccagcgcgcg gaccgactct
     3841 gtgcccaccg gtacgttgga cgaccccgcg tgcggccagg cccgatcgtc gcggttctcg
     3901 gcagggcccg gctcaccgct gccgattgtc tgcggctgca ccggccctga gaacgagccc
     3961 ggcggaatgc tcacaggccg tcctgatcgc cgatgccgat ggcgatcaac cggtcgcgca
     4021 gcgcttcggc gtcacgttgc ggcagtcccg gcacccggcc acccgattgc aggctggccg
     4081 tgaccagttc gacgttggcc aggccgagcg cgcgggtcaa cgggccgctc tcgaccttca
     4141 ccgcctgcat gcggccgtac ggaatgacga tcagctcacg cgaccagagc ccgtggctaa
     4201 ggtacaggtc gtcgtcacgt tcgaggtagc cccaggcccg cacccaacgc ccgagcctga
     4261 accactgcca ccctgcgtag ccgacgccca gggcgatcag ggcacccgcc cagcgggggt
     4321 cgacggcatg ccacaacacg gtggccacca cgccgatcac aagggcggta ccgatcagca
     4381 tgcgtgcgcg cagcaccatg gcgtaggcgg gcgagatgcg ccgccagccg acagccggcg
     4441 gcgcgaacag gtcgtccatg tcgttcagcg tacgggccga ccccgcacgg ccgagcgggg
     4501 cgggggcgtc catctgggac ggctcagctg ccgcagtcgc ggcgcttgcg ttgcttcttg
     4561 ctgaggccgc gcacctcgac cccgccgaac ccgatgaagc ccttgacgat cacggtgggc
     4621 gcgccgggcg tgggaggcat ggtcttggtc tcggagccac cgaagaccgc catgcactcg
     4681 ttgcgcaccg tggtgccgtc gggcaccgtg atctcgatgc cgccgaacag gcagaacacg
     4741 ttgatctcgc acacgttgtc ggtgaagacg gcctgggtga ggtcgagttc cgccccgccg
     4801 aacagggtca gcacgctgag attgcgccgc ggctgccaca cgcctttgcg agcggcgccg
     4861 ctgaagatgg cgacgatcat ctcggggccg gtgtcggggc cgaccggtgc ggacgaccag
     4921 gtcggggtcg cggccgggcc gggcaccacc acgaggtcac gggtgagcgg caccagatcg
     4981 tcgaaggtct gggcctgatt ggcggcctgc gtgcgctcgg catgctcggc cggggtgatg
     5041 cggccttcgg ccagggcggc gtcgagcagg gtgagcacct tggtcttgtc gctcgcgttc
     5101 acggccaggt cgccgccgta ggcgggcccg acgtctgtcg gttcggcgta actcatggca
     5161 ctgacgctag ccccgtgcgg cgaccatggg cacccgggcg aggagtctcc ggggcacaga
     5221 cgggggacac cctgagccgt tgtgcggcgg ccacgacgcg cccgctagac tgacggcggt
     5281 cagcgcgcct cgcgggcgta gttcaatggc agaacatcag cttcccaagc tgagaatgcg
     5341 ggttcgattc ccgtc
//
LOCUS       DAOSUG010000169         5344 bp    DNA     linear   ENV 10-JUN-2024
DEFINITION  MAG TPA_asm: Micropruina sp. isolate AMR_MDS_2195
            NODE_6904_length_5344_cov_3.008697, whole genome shotgun sequence.
ACCESSION   DAOSUG010000169 DAOSUG010000000
VERSION     DAOSUG010000169.1
DBLINK      BioProject: PRJNA925477
            BioSample: SAMN32864262
            Sequence Read Archive: SRR4236673
KEYWORDS    WGS; ENV; Metagenome Assembled Genome; MAG; Third Party Data; TPA;
            TPA:assembly.
SOURCE      Micropruina sp. (wastewater metagenome)
  ORGANISM  Micropruina sp.
            Bacteria; Bacillati; Actinomycetota; Actinomycetes;
            Propionibacteriales; Nocardioidaceae; Micropruina.
REFERENCE   1  (bases 1 to 5344)
  AUTHORS   Abdulkadir,N., Saraiva,J.P., Zhang,J., Stolte,S., Gillor,O.,
            Harms,H. and Rocha,U.
  TITLE     Genome-centric analyses of 165 metagenomes show that mobile genetic
            elements are crucial for the transmission of antimicrobial
            resistance genes to pathogens in activated sludge and wastewater
  JOURNAL   Microbiol Spectr 12 (3), e0291823 (2024)
   PUBMED   38289113
REFERENCE   2  (bases 1 to 5344)
  AUTHORS   Abdulkadir,N.
  TITLE     Direct Submission
  JOURNAL   Submitted (24-MAR-2023) Environmental Microbiology, Helmholtz
            Center for Environmental Research -UFZ, Permoserstr 15, Leipzig
            04318, Germany
COMMENT     The annotation was added by the NCBI Prokaryotic Genome Annotation
            Pipeline (PGAP). Information about PGAP can be found here:
            https://www.ncbi.nlm.nih.gov/genome/annotation_prok/
            
            ##Genome-Assembly-Data-START##
            Assembly Method        :: SPAdes v. 3.11.1
            Genome Representation  :: Full
            Expected Final Version :: Yes
            Genome Coverage        :: Not Applicable
            Sequencing Technology  :: Illumina MiSeq
            ##Genome-Assembly-Data-END##
            
            ##Genome-Annotation-Data-START##
            Annotation Provider               :: NCBI
            Annotation Date                   :: 05/04/2023 04:57:10
            Annotation Pipeline               :: NCBI Prokaryotic Genome
                                                 Annotation Pipeline (PGAP)
            Annotation Method                 :: Best-placed reference protein
                                                 set; GeneMarkS-2+
            Annotation Software revision      :: 6.5
            Features Annotated                :: Gene; CDS; rRNA; tRNA; ncRNA
            Genes (total)                     :: 2,776
            CDSs (total)                      :: 2,750
            Genes (coding)                    :: 2,714
            CDSs (with protein)               :: 2,714
            Genes (RNA)                       :: 26
            tRNAs                             :: 24
            ncRNAs                            :: 2
            Pseudo Genes (total)              :: 36
            CDSs (without protein)            :: 36
            Pseudo Genes (ambiguous residues) :: 0 of 36
            Pseudo Genes (frameshifted)       :: 2 of 36
            Pseudo Genes (incomplete)         :: 32 of 36
            Pseudo Genes (internal stop)      :: 2 of 36
            ##Genome-Annotation-Data-END##
FEATURES             Location/Qualifiers
     source          1..5344
                     /organism="Micropruina sp."
                     /mol_type="genomic DNA"
                     /submitter_seqid="NODE_6904_length_5344_cov_3.008697"
                     /isolate="AMR_MDS_2195"
                     /isolation_source="Activated sludge of municipal
                     wastewater treatment plants"
                     /db_xref="taxon:2737536"
                     /environmental_sample
                     /geo_loc_name="Canada"
                     /lat_lon="49.25 N 123.1 W"
                     /collection_date="2015-06-16"
                     /metagenome_source="wastewater metagenome"
                     /note="metagenomic"
     gene            140..>689
                     /locus_tag="PLF56_06780"
     CDS             140..>689
                     /locus_tag="PLF56_06780"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_013865566.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_component: GO:0016020 - membrane [Evidence IEA];
                     GO_process: GO:0055085 - transmembrane transport [Evidence
                     IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="ABC transporter permease subunit"
                     /protein_id="HOQ53303.1"
                     /translation="MKQLSPQRRRIFAPLALGVLLLAAWTIAAAVASPVLLPGPARVL
                     VRLWTDLLTGALWPYLAVTVAEALGGCLLGAVVALPLAVTIHRSVWFSDAVQPFLGAT
                     QAIPAIALAPLLVLWVGYGLVPVVLLCALIVFFPILVAAVVGLRMVPRDVVDAARLDG
                     AGSLELLRHVEAPLALPSLLGGF"
     assembly_gap    690..699
                     /estimated_length=10
                     /gap_type="within scaffold"
                     /linkage_evidence="paired-ends"
     gene            <700..906
                     /locus_tag="PLF56_06785"
     CDS             <700..906
                     /locus_tag="PLF56_06785"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_013865566.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="ABC transporter permease"
                     /protein_id="HOQ53304.1"
                     /translation="FALSVTGAVVGEMVMGGSGLGTVLTVQRDAVDTTGMFATIAWLC
                     LIAAAAYSLITGWEHRSAIVEALT"
     gene            999..2051
                     /locus_tag="PLF56_06790"
     CDS             999..2051
                     /locus_tag="PLF56_06790"
                     /inference="COORDINATES: protein motif:HMM:NF020650.2"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="ABC transporter substrate-binding protein"
                     /protein_id="HOQ53305.1"
                     /translation="MPMPDPTSLRLRRRTLLSAALALAAAGCTGPSAGPSGSPSAATT
                     TPTASGVRATIGLSYIPNVQFAPFYVAEADGLFDGVSAEVTLRHHGATEGLFTAFSSG
                     TEDFLIAAGSEAMQARAEGVDVVAVGSYFKAFPAQVLVLDASPIRSLADLEGRSLGVP
                     GRYGESWLAAQVALRTAGLSEADLRIVEVGYTQVAALTTKKVDAVIGFSNNELVQLEA
                     SGAKARSLPVATGTVPLVSASLLTSSAYAKAHPEVVRQVVAAMTKGIERTAADTGRAL
                     DVSAAQIPGWTAAARPNAEAVLNATLALMSLREGVFVPALDPDQWTAMARFLDTAGLI
                     KAQVSAADAFTNEYAR"
     gene            2339..4591
                     /locus_tag="PLF56_06795"
     CDS             2339..4591
                     /locus_tag="PLF56_06795"
                     /inference="COORDINATES: protein motif:HMM:NF014567.2"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="ATP-binding protein"
                     /protein_id="HOQ53306.1"
                     /translation="MSPYRRAGSLGRADPARGATASGLRLWLARAVAMTCLASLLPAL
                     FLSLTQLPHLNLVWLVLVGGGIVAFSLLMPVYAWASGDIRWLCGLYAVVNLIGLATWP
                     LAWPSGVVGGAPWLWVCLGVATVCAAMATTVIVAFGYAAVSALIFGTVRMLPSGGSVS
                     ALTAMQDMLTLVVLPTALLLLIQYFARAVEELDATTAESQRVEADRAIDKALTQRRVI
                     LDGIIHDQVMTTLVAAVQTDHPRGEVADLARSAVTSLAEAAAPTDVGQPLSPHQLARL
                     IEDMVEPVCPQAKVSVASDSDAIAVSPSVASTLGQAAREAALNVTRHAEADQVTVEIA
                     ARLVGDDQRVQVDIRDNGRGFDPEAVDAGRFGIKVSMHERLAFIGGAVDIDSEPGTGT
                     LVRLLWSGRDQYAGETISRRRASQALRSVLRVELFLFLVWLVVGVQVLVGLISVLAIP
                     NPLPILIAQGLAVVATAIALHKAGERNMTTTEAGVVVLCLAVIAHLVHAGVPGAQVPG
                     YLTWHSSVIMVLLITVLVRGQRLAAWCGLAVFIVQVLIWARGNELGASAVLQVIFGPV
                     LWLTLATLVLRGLRSIGEQLVRVRTRSRENTRAMAESFSNLVLREVWLTQLREQIGPL
                     LQNLADPSHQLSQPERDACRILEGRLRDALRAGNLVSQGVSDAIQAARGRGVEVVLVD
                     NRGSKLPENVRLATLRQLEELVATSTGGRIVARTAPEGYSDAVTILKVDAERNLTTID
                     EAGTVTVRKT"
     gene            4588..5250
                     /locus_tag="PLF56_06800"
     CDS             4588..5250
                     /locus_tag="PLF56_06800"
                     /inference="COORDINATES: protein motif:HMM:NF012301.2"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_function: GO:0003677 - DNA binding [Evidence IEA];
                     GO_process: GO:0000160 - phosphorelay signal transduction
                     system [Evidence IEA];
                     GO_process: GO:0006355 - regulation of DNA-templated
                     transcription [Evidence IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="response regulator transcription factor"
                     /protein_id="HOQ53307.1"
                     /translation="MSASSTIRAAIIDDHEAIRLGVGAALQQHTAGHVRVVSSHENVD
                     DFLADEVEADVVMLDLSLGDDTDPKENVERLAEVGYRILVYSIADNMYLVRRALAGGA
                     DGVCRKAEPIAQTIEALEAVANGQMVLSQEILGAIESDTSYVAASLGPRERQVLSLYV
                     AGLEIPEVAQQLYITENSVKEYLKRIRVKYTQVNRPANNKLELFRRAIEDGIIPPVER
                     RG"
BASE COUNT          725 a         1831 c         1881 g          897 t
ORIGIN      
        1 ttgatccggt gaaactccgg aaccgacggt gaaagtccgg atgggaggca gcacgtgcgg
       61 tgggcgtcgt cggcgtccgc cgtcgttggc gcattctcct gcgccatccc cgggtcgtga
      121 agctgacccg gaggagacca tgaagcaact cagcccccag cggcggcgca tcttcgcgcc
      181 gttggctctc ggggtgctgc tgctggccgc ctggacgatc gcggcagccg ttgcgtcgcc
      241 cgtgctgctg cccggtcccg cgcgcgtgct cgtccggctc tggacggacc tgctcacggg
      301 cgcgctgtgg ccctacctgg cggtcaccgt cgccgaggcg ctgggcggct gcctgctcgg
      361 cgccgtggtc gcgcttccct tggccgtcac cattcatcgt tcggtctggt tcagcgacgc
      421 cgtccagccg tttctgggtg ccacccaggc cattccggcc atcgcgctgg ccccgctgct
      481 ggtgttgtgg gtcggttacg ggctggtgcc ggtggtgctg ctgtgcgccc tgatcgtgtt
      541 ctttccgatc ctggtggccg ccgtggtcgg gttgcgcatg gtgccccgcg acgtggtgga
      601 cgccgcccgg ctcgacggcg cgggcagcct ggagttgctg cgccacgtcg aggcaccgct
      661 ggcactgccc tcgctgctcg gtggcttcan nnnnnnnnnt tcgcgctgag cgtcaccggt
      721 gccgtggtgg gcgagatggt gatgggcggt agcgggctcg gcaccgtgct gaccgtgcag
      781 cgcgacgccg tcgacaccac cggcatgttc gcgaccatcg cctggctgtg cctgatcgcc
      841 gccgccgcct actcattgat caccgggtgg gagcatcgct cggccatcgt cgaggccctg
      901 acctgacctg acccgactga cctggcccga accgagctga cctgacctga cctgactgac
      961 ctgacccgac cccagctgac cgacccgagg agcgtccgat gccaatgccc gacccgacca
     1021 gcctgcggct gcgtcgccgc accctgctga gcgccgccct cgccctcgcc gccgccggct
     1081 gcaccggtcc gtccgccgga ccctcaggct cgccgagcgc cgcgaccacc acccccaccg
     1141 cctccggggt gcgcgcgacg atcgggctca gctacattcc caacgtccag ttcgcgccgt
     1201 tctatgtcgc cgaggcggac gggctgttcg acggggtcag tgccgaggtc accctgcgtc
     1261 atcacggcgc caccgaggga ctgttcaccg ccttctcctc cggcaccgag gactttctga
     1321 tcgcggcggg cagcgaagcg atgcaggccc gcgcggaagg cgtcgacgtg gtcgccgtcg
     1381 gctcgtactt caaggccttt ccggcgcagg tgctggtgct ggacgcgtcc ccgatccgtt
     1441 cgctcgccga cctcgagggc cgctcgctgg gcgtgccggg tcgctacggc gagtcgtggc
     1501 tggccgccca ggtggcgttg cgcacggccg ggctgagcga ggccgacctg cggatcgtcg
     1561 aggtcggcta cacccaggtg gcggccctga ccactaagaa ggtggacgcc gtgatcggct
     1621 tcagcaacaa cgaactcgtc cagttggagg cctcgggcgc caaagcccgc tcgctgccgg
     1681 tggccaccgg cacggtgccg ctggtcagcg cgtcgctgct cacctcgagc gcctacgcga
     1741 aggcgcatcc cgaggtcgtg cgccaggtgg tcgccgcgat gaccaagggc atcgagcgca
     1801 ccgccgccga cacggggcgg gcgctggacg tgtcggccgc ccagattccc ggttggaccg
     1861 ctgcggccag gcccaacgcc gaagcggtgc tgaacgccac cctggcgttg atgtcgctga
     1921 gggaaggcgt cttcgttccc gccctcgacc cggatcagtg gacggccatg gcacggtttc
     1981 tcgacacggc cggcttgatc aaggcgcagg tgagcgctgc ggacgccttc accaatgagt
     2041 acgcgcgctg accggtgtgc cgacgcatga gcgtggcggc tatgggtcgg gacgcacacc
     2101 gtccgcgacg caccccgtcc ggagccttgc tgatgcgaac ggctcgcagt gccggtggcg
     2161 tgccggccga cgaaggggtg ggccactacg cccgttttct ggccgctgtc ggtgccggct
     2221 ggcagtatga cttcatgacg ctccaaccgc tcggccgggc gtgcctcgtc gtgaccggac
     2281 cggcggccgc gggcgggtcg tcggccactg cggggcggcc gcaggtccgg gccgcatagt
     2341 gtccccctac aggcgagccg gcagcctggg cagggccgat cccgcccggg gcgccacggc
     2401 gtccggactg cggctatggc tggcccgggc ggtcgccatg acctgcctcg ccagcctgct
     2461 gccggccctg ttcctctcgc tcacccagct gccccacctg aacctggtgt ggttggtgct
     2521 ggtgggcggc ggcatcgttg cgttcagcct gctgatgccc gtgtacgcat gggcgtccgg
     2581 cgacattcgc tggttgtgcg ggctgtacgc cgtggtcaac ctgatcggcc tcgccacctg
     2641 gccgttggcg tggcccagcg gtgttgtcgg cggggcgccc tggctgtggg tgtgtctggg
     2701 tgtggccacg gtgtgtgccg cgatggccac cacggtgatc gtcgcgttcg gctatgcggc
     2761 ggtgtcggcg ttgatcttcg gcaccgtgcg catgctgccc tccggcggct cggtatcggc
     2821 gctcaccgcc atgcaggaca tgctcaccct ggtggtgttg ccgaccgccc tgctgctgct
     2881 gatccagtac ttcgcgcgcg cggtcgaaga gctggacgcc accactgccg agtcccagcg
     2941 tgtcgaggcc gatcgagcga tcgacaaggc cctgacccag cgacgggtga tcctcgacgg
     3001 catcattcac gatcaggtga tgaccaccct ggtggcggcc gtccagaccg atcatccgcg
     3061 tggcgaggtg gccgatctcg cccgctcggc ggtcacgtcc ctggccgagg ccgccgcacc
     3121 gaccgatgtc ggccagccgc tgtcaccgca tcagctggcc aggttgatcg aagacatggt
     3181 cgagccggtg tgtccgcagg ccaaggtgag cgtcgcctcc gacagcgacg cgatcgccgt
     3241 cagcccgtcg gtcgccagca cgctggggca ggcagcgcgc gaggcggcgc tcaacgtcac
     3301 ccggcacgcc gaggccgacc aggtcacagt cgagatcgct gcgcggctgg tgggcgacga
     3361 ccagcgcgtg caggtcgaca tccgcgacaa cggacgcggc ttcgatcccg aagcggtcga
     3421 tgccggccgc ttcggtatca aggtgtcgat gcacgagcgt ctggccttca tcggcggcgc
     3481 cgtcgacatc gactccgaac cgggcaccgg caccctggtc aggctgctct ggtccggacg
     3541 cgatcagtac gcaggcgaga ccatctcgcg ccggcgggcg tcccaggcgt tgcggtcggt
     3601 gctgcgggtc gagctgttcc tgttcctggt gtggctggtg gtgggcgtgc aggtgctggt
     3661 cggcctgatc tcggtgttgg cgatcccgaa tccgctgccg atcctgatcg cgcaggggct
     3721 cgccgtcgtg gccaccgcca tcgcgctgca caaggcgggc gagcgcaaca tgaccaccac
     3781 cgaggccggg gtggtggtgc tgtgcctcgc cgtgatcgca cacctggtgc acgcgggagt
     3841 gccgggggcc caggtgccgg gctacctgac ctggcacagc tcggtgatca tggtgctgct
     3901 gatcaccgtg ctggtgcgcg ggcagcgact ggcggcctgg tgcgggctgg ccgtgttcat
     3961 cgtgcaggtg ctcatctggg cccggggcaa cgaactcgga gcgtccgcgg tactgcaggt
     4021 gatcttcggt ccggtgctct ggctcaccct cgccacgctg gtgctgcgcg gcctgcggtc
     4081 gatcggtgaa cagttggtgc gggtgcgcac ccgctcacgc gagaacaccc gtgccatggc
     4141 cgagagcttc tcgaatctgg tgctgcgcga ggtgtggctg acccagttgc gcgagcagat
     4201 cggcccgttg ctgcagaacc tggccgaccc gtcccatcaa ctcagccagc ccgagcgtga
     4261 cgcctgccgc attctcgagg gccggctgcg cgatgccctg cgggccggca accttgtttc
     4321 acaaggggta tcggacgcca ttcaggcggc ccgcggccgg ggggtcgagg tggtgctggt
     4381 cgacaaccgc ggctcgaagt tgcccgagaa cgtccgattg gccacgttgc gccagctgga
     4441 agagctggtg gcaacctcca ccggtggacg tatcgtggct cgcaccgcac ccgagggcta
     4501 ctcggacgcg gtgacgattc ttaaagtcga cgcggagcgt aacctgacta cgatcgacga
     4561 ggccggcacc gtcacagtga ggaagacatg agcgcatcca gcacgattcg ggcggcgatc
     4621 atcgacgatc acgaagcgat ccggctgggc gtcggcgcgg ccctgcagca gcacaccgcg
     4681 ggccacgtgc gggtggtcag ttcacatgag aacgtggacg actttctggc cgacgaggtc
     4741 gaggccgacg tggtgatgct cgacctgtcg ctcggcgacg ataccgaccc caaagagaac
     4801 gtggagcggc tggccgaggt cggctaccgc attctggtgt actcgatcgc cgacaacatg
     4861 tacctggtgc gccgggcgct ggccggcgga gccgacggtg tgtgccgcaa ggccgagccg
     4921 atcgcgcaga ccatcgaggc gctcgaggcg gtcgccaacg gccagatggt gctgtcgcaa
     4981 gagatactgg gcgcgatcga gtccgacacc agctatgtgg cggcgagcct cggcccgcgc
     5041 gagcggcagg tgctgtcgct gtatgtggcg ggtctcgaga ttcccgaggt ggcgcagcag
     5101 ttgtacatca ccgagaacag cgtcaaggag tatctcaaac gcatccgggt gaagtacacc
     5161 caggtcaacc gcccggcgaa caacaagctg gaactgttcc ggcgagccat cgaagacggc
     5221 atcattccgc cggtggaacg ccgaggctga gtcggtcatg gggcgtccgt cgtgccccca
     5281 cgtccgtcct cccggcgtgc cccacctccg tcatcccggc gtgccccacc tccgtcatcc
     5341 cggc
//
LOCUS       DAOSUG010000170         5343 bp    DNA     linear   ENV 10-JUN-2024
DEFINITION  MAG TPA_asm: Micropruina sp. isolate AMR_MDS_2195
            NODE_6905_length_5343_cov_3.880862, whole genome shotgun sequence.
ACCESSION   DAOSUG010000170 DAOSUG010000000
VERSION     DAOSUG010000170.1
DBLINK      BioProject: PRJNA925477
            BioSample: SAMN32864262
            Sequence Read Archive: SRR4236673
KEYWORDS    WGS; ENV; Metagenome Assembled Genome; MAG; Third Party Data; TPA;
            TPA:assembly.
SOURCE      Micropruina sp. (wastewater metagenome)
  ORGANISM  Micropruina sp.
            Bacteria; Bacillati; Actinomycetota; Actinomycetes;
            Propionibacteriales; Nocardioidaceae; Micropruina.
REFERENCE   1  (bases 1 to 5343)
  AUTHORS   Abdulkadir,N., Saraiva,J.P., Zhang,J., Stolte,S., Gillor,O.,
            Harms,H. and Rocha,U.
  TITLE     Genome-centric analyses of 165 metagenomes show that mobile genetic
            elements are crucial for the transmission of antimicrobial
            resistance genes to pathogens in activated sludge and wastewater
  JOURNAL   Microbiol Spectr 12 (3), e0291823 (2024)
   PUBMED   38289113
REFERENCE   2  (bases 1 to 5343)
  AUTHORS   Abdulkadir,N.
  TITLE     Direct Submission
  JOURNAL   Submitted (24-MAR-2023) Environmental Microbiology, Helmholtz
            Center for Environmental Research -UFZ, Permoserstr 15, Leipzig
            04318, Germany
COMMENT     The annotation was added by the NCBI Prokaryotic Genome Annotation
            Pipeline (PGAP). Information about PGAP can be found here:
            https://www.ncbi.nlm.nih.gov/genome/annotation_prok/
            
            ##Genome-Assembly-Data-START##
            Assembly Method        :: SPAdes v. 3.11.1
            Genome Representation  :: Full
            Expected Final Version :: Yes
            Genome Coverage        :: Not Applicable
            Sequencing Technology  :: Illumina MiSeq
            ##Genome-Assembly-Data-END##
            
            ##Genome-Annotation-Data-START##
            Annotation Provider               :: NCBI
            Annotation Date                   :: 05/04/2023 04:57:10
            Annotation Pipeline               :: NCBI Prokaryotic Genome
                                                 Annotation Pipeline (PGAP)
            Annotation Method                 :: Best-placed reference protein
                                                 set; GeneMarkS-2+
            Annotation Software revision      :: 6.5
            Features Annotated                :: Gene; CDS; rRNA; tRNA; ncRNA
            Genes (total)                     :: 2,776
            CDSs (total)                      :: 2,750
            Genes (coding)                    :: 2,714
            CDSs (with protein)               :: 2,714
            Genes (RNA)                       :: 26
            tRNAs                             :: 24
            ncRNAs                            :: 2
            Pseudo Genes (total)              :: 36
            CDSs (without protein)            :: 36
            Pseudo Genes (ambiguous residues) :: 0 of 36
            Pseudo Genes (frameshifted)       :: 2 of 36
            Pseudo Genes (incomplete)         :: 32 of 36
            Pseudo Genes (internal stop)      :: 2 of 36
            ##Genome-Annotation-Data-END##
FEATURES             Location/Qualifiers
     source          1..5343
                     /organism="Micropruina sp."
                     /mol_type="genomic DNA"
                     /submitter_seqid="NODE_6905_length_5343_cov_3.880862"
                     /isolate="AMR_MDS_2195"
                     /isolation_source="Activated sludge of municipal
                     wastewater treatment plants"
                     /db_xref="taxon:2737536"
                     /environmental_sample
                     /geo_loc_name="Canada"
                     /lat_lon="49.25 N 123.1 W"
                     /collection_date="2015-06-16"
                     /metagenome_source="wastewater metagenome"
                     /note="metagenomic"
     gene            complement(7..168)
                     /locus_tag="PLF56_06805"
     CDS             complement(7..168)
                     /locus_tag="PLF56_06805"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_013160897.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="DUF5679 domain-containing protein"
                     /protein_id="HOQ53308.1"
                     /translation="MADETYSGSFYCVKCKDHRDATGDVTVNAKGTRIAKAKCPVCGT
                     NLTRFLPKA"
     gene            518..1048
                     /locus_tag="PLF56_06810"
     CDS             518..1048
                     /locus_tag="PLF56_06810"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_013864032.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="M48 family metallopeptidase"
                     /protein_id="HOQ53309.1"
                     /translation="MASEAYQVRRSARRRSTMGIMREGGSLVVVVPQHLTRTQERELV
                     PAFVSRYLQNEAARRPPAGDAALTARAQQLFAAHLAEPGQPRPTFSVAWSNRQQQRWG
                     SCTTATGEIRLSERLRVMPDWVADYVLMHELSHLLERTHTARFHALVARYPQCERAQG
                     FLEGWQAALNRPSIEC"
     gene            complement(1065..2426)
                     /locus_tag="PLF56_06815"
     CDS             complement(1065..2426)
                     /locus_tag="PLF56_06815"
                     /inference="COORDINATES: protein motif:HMM:TIGR03624.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="zinc-dependent metalloprotease"
                     /protein_id="HOQ53310.1"
                     /translation="MATDPDLPSDSDNPGEQNPLAQFLAQFGITPGPDGEFDLQALTS
                     RLQSVMAQFQTQMAGFGPADAASGMNWTFTRDIARRVTAAKGADTTPSAAQGAGVRDA
                     AGLADLWLDEEIAFARLSTPAVAWSRADWIEGTFATWQQLSNPIVTSLSAALGSMMAR
                     ETGPGEEFMKPMVRAAASGMFAAQVGQSLGSLATEVLSVSDIGLPLGERPQVALLPTN
                     IELFGADLDNVTPADVLLFLALRETARQRLFAAVGWLGPQLHALVEHYARDITIDTTA
                     LEEAIESQLSSAMSADDLERAGQTLAGKLFEPAVTAEQAEVLERLETLLALVEGWVDE
                     VVGHTMSRWMPNGAALTEVVRRRRAAGGPAEAALKSLVGLELRPRRTRDAANLWAAIR
                     ATRGAAERDAAWAHPDLIPTSADLDDPLGYAEHGHVAPAPDDLDAELAKLLDEEAGGA
                     KDQ"
     gene            2567..3616
                     /locus_tag="PLF56_06820"
     CDS             2567..3616
                     /locus_tag="PLF56_06820"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_015070696.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_function: GO:0005515 - protein binding [Evidence IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="PDZ domain-containing protein"
                     /protein_id="HOQ53311.1"
                     /translation="MTKQTWTAAVSALLFVALAAVVSLVPVPFVSWSAGSTVNLFEPD
                     NGQPRVDIQGIQTYPTTGQLRLTTVSVTRADSQLTLPEALLSYWLSAREVLPREAIYP
                     AGASVDDIRAREAQMMDNSQSAAVAAALRAAGKRVVEYPMVVGVSNGSPADGRLKPGD
                     FILRVDNKAVSSTEAVRAAINTHQVGEAIVFTVLRDRQQLNETITTVASNASATTPVV
                     GINLTIGYSYAPTVTFRISTDIGGPSAGLVFALAVYDAINPDNLIDGRSVAGTGTITG
                     NGEVGAIGGIQEKVAGAEAAGATIFLVPSDNCADVQGVSTAVRLVRVATLTDAIDALT
                     ALKDPARAAGVRGCE"
     gene            3613..4125
                     /locus_tag="PLF56_06825"
     CDS             3613..4125
                     /locus_tag="PLF56_06825"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_016670218.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="PPA1309 family protein"
                     /protein_id="HOQ53312.1"
                     /translation="MSTSDDLVAGLLEIEHHVSGLGWDQPARLFALVPTTELIAAEPQ
                     LAQHLTGGTAPRPGHLSAVEQEGFAGGDDLGEALARISWPATVAGVALSLERLFLPGS
                     ADTDLPPGPTGDEQARRHPAREEIRVVVGALRGGDSYGVARIRSHPDELLSGSDLVPG
                     LAAALARTLD"
     gene            4151..>5343
                     /locus_tag="PLF56_06830"
     CDS             4151..>5343
                     /locus_tag="PLF56_06830"
                     /inference="COORDINATES: protein motif:HMM:NF015644.2"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_component: GO:0016020 - membrane [Evidence IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="UPF0182 family protein"
                     /protein_id="HOQ53313.1"
                     /translation="MSLSASVHRRSPILLTLGIAAVAIIAFAVFTQVWTDKLWFESIG
                     FGTVFTTELLTRLGLFFGVAIVMALLIGGNMWLAIRLRPRGRRAGDSAVLDAYRRLLE
                     SNARIAVLGPALAFGLLAGMSATTEVLPLLAWINAVPFGRTDPVFGLDYSFYVFDFPV
                     WQSLLSFLLTALIFSLIAAALVHFAMGGIRFGRSPGRPQFSQAARVHLSVLTGVLLVL
                     YGLQCLLDRYGMLLSQGTLFTGVHYTDDHARIGAKLVMAVISFLVAGLFFANPILKRG
                     IYPAAALVLMVISGLILTLIYPAIVQSFVVAPNQPDKENPYMQSHINMTRDAFGIADT
                     EVTDYEAVTQVKAGQLKADAEALPGIRLMDPAVIGPTYEQLQQVRGYYTFPDVLDVDR
                     YTIDG"
BASE COUNT          781 a         1853 c         1809 g          900 t
ORIGIN      
        1 gtgggttcag gccttgggca ggaagcgggt caggttcgta ccgcaaacgg ggcacttggc
       61 cttcgcgatc cgggttccct tcgcgttcac cgtcacgtcg ccggtggcgt cgcggtggtc
      121 cttgcacttc acgcagtaga acgaaccgct gtaggtttcg tcggccacgt cattcctcct
      181 attgatgggg tgcgctggag caaccacagc gcgtttgcag gtcgagcata cggtacccac
      241 cgccttgagg gcgcacccct tggggcgcgg cacccgaggc gacgacaccg ctccgaccgc
      301 cgaccgcacg gtctgccgag aaatcgaagc ccggtgccca agaaaacgaa gctcgccggg
      361 aacttttccg cttgccttcc cctgcgaacg gatcgtgccc cagcgagcct cgtcgctcag
      421 acggtagtgg tgccgagcct gcgtggtcaa cacgacgatc gaggctcggg ccgagtctgt
      481 acaggtttgt gtccgggggc ccgtttaggg tgtctgcatg gcatccgagg cttatcaggt
      541 gcgccgcagc gcccggcgcc gcagcaccat gggcatcatg cgtgagggtg gctccctggt
      601 ggtcgtggtg ccccagcacc tgacccgcac gcaagagcgc gagttggtgc ccgccttcgt
      661 ctcgcgctac ctgcagaacg aggcggcgcg acgtccgccg gccggcgatg ccgcgctcac
      721 cgcgcgggca cagcagctgt tcgcggcgca cctggccgaa ccggggcagc ctcgtccaac
      781 gttcagcgtg gcgtggtcga accggcagca gcaacggtgg ggctcctgta ccaccgccac
      841 cggcgagatc cggttgtccg agcggctgcg cgtgatgccc gactgggtgg ccgactacgt
      901 gctgatgcat gaactcagtc acctgctcga acgcacccac acggcacggt tccatgccct
      961 ggtcgcccgc tacccgcagt gcgaacgcgc gcagggcttt ctcgaaggtt ggcaggctgc
     1021 gctgaaccgg cccagcatcg agtgctgacc accggtacta cccgttactg gtccttcgcg
     1081 ccgcccgctt cctcatcgag cagtttggcc agttcggcgt ccaggtcgtc gggtgccggc
     1141 gccacatggc cgtgctcggc gtagccgagg ggatcgtcca ggtcggctga ggtcggaatc
     1201 aggtcggggt gagcccacgc cgcgtcccgt tccgcggcgc cccgagtggc gcggatcgcc
     1261 gcccacaggt tggctgcgtc ccgggttcgt cgcggccgca actccaggcc caccagcgac
     1321 ttcagcgcgg cctcggcggg gccgccggcg gctcgccgcc tgcgcaccac ctcggtcagg
     1381 gcggccccgt tgggcatcca ccggctcatc gtgtggccga ccacctcatc gacccaaccc
     1441 tcgaccaggg cgagcagggt ctcgagccgt tccagcacct cggcctgctc ggcagtgacc
     1501 gccggttcga acagcttgcc cgccagggtc tgccccgcgc gctccaggtc gtcggcgctc
     1561 atcgccgagc tgagctgcga ctcgatcgct tcttcgagtg ccgtggtgtc gatcgtgatg
     1621 tcgcgggcat agtgctcgac cagggcgtgc agctgcggcc ccagccagcc gaccgcagcg
     1681 aagagccgtt gccgggcggt ctcgcgcagg gcgaggaaca gcagcacgtc ggccggggtg
     1741 acgttgtcga gatcggcgcc gaacaactcg atgttggtgg gcagcagcgc cacctgcgga
     1801 cgttccccca acggcaaccc gatgtcgctg acgctcagca cttcggtggc gaggctgccc
     1861 agcgactggc cgacctgggc ggcgaacatg ccggacgccg cggcgcgcac catcggcttc
     1921 atgaactcct cgcccggacc cgtctcgcgc gccatcatcg aacccagcgc cgccgacagg
     1981 ctggtgacga tcgggttgct cagctgttgc caggtcgcga acgtgccctc gatccagtcg
     2041 gcccggctcc acgccaccgc cggggtgctc aaccgggcga aggcgatctc ctcgtccagc
     2101 cacaggtcgg ccaggccggc ggcgtcccgg acgccggcgc cctgtgccgc cgacggcgtg
     2161 gtgtcggcgc ccttggcggc cgtcacccgg cgggcgatgt cgcgggtgaa ggtccagttc
     2221 atgccgctgg ccgcatccgc gggcccgaaa ccggccatct gggtctggaa ctgcgccatc
     2281 accgactgca gtcgcgaggt gagcgcctga aggtcgaact cgccgtccgg acctggtgtg
     2341 atgccgaact gggcgagaaa ctgggccagc gggttctgct cgccagggtt gtcgctgtcg
     2401 ctgggcaggt ccgggtcggt ggccatgagt ggctcctgat tgcgttgtcg gattcgatgc
     2461 cggacgcagc aactcacggc gtccggccgg tcgtcaccat tgttccgcat ctggcaagcg
     2521 cagttcgggg cagagcgaca cgaacccgtc cggtagtgtc aatgaggtga ccaagcagac
     2581 ctggacggcc gcggtctcgg ccctgctctt cgtggcgttg gcggccgtgg tctcgctcgt
     2641 gccggtgccg ttcgtgagtt ggtcggccgg ctccaccgtc aacctattcg aacccgacaa
     2701 cggtcagccg cgggtcgata ttcagggcat ccagacctat cccacgaccg gtcagctccg
     2761 cctgaccacg gtgtcggtga cccgtgccga cagtcagttg acgcttcccg aggcgctgct
     2821 gtcgtactgg ctctcggctc gcgaggtgct gccgcgcgag gcgatctacc cggcgggcgc
     2881 cagcgtggac gacatccgcg cacgcgaggc gcagatgatg gacaactccc agtcggcggc
     2941 ggtcgcggcg gcgctgcgtg cggcgggcaa gagggtcgtc gaatacccga tggtggtggg
     3001 agtgtcgaac ggcagcccgg ctgatggccg gctcaagccc ggcgacttca ttctgcgggt
     3061 cgacaacaag gcggtcagct cgaccgaagc ggtccgggcc gcgatcaaca cccatcaggt
     3121 cggcgaggcc atcgtgttca ccgtcctgcg tgatcgccag caactcaacg aaaccatcac
     3181 caccgtggcg tccaacgcca gtgcgaccac gccggtggtg ggcatcaatc tcaccatcgg
     3241 gtactcctac gcacccacgg tcaccttccg gatcagcacc gacatcggcg gtccgagcgc
     3301 ggggttggtg ttcgccctcg ccgtctacga cgcgatcaac cccgacaacc tgatcgacgg
     3361 acgctccgtg gccggcaccg gcacgatcac cggtaacggc gaggtgggtg cgatcggcgg
     3421 catccaggag aaggtggcgg gtgccgaggc ggccggggcc acgatctttc tggtgcccag
     3481 cgacaactgc gccgacgtgc agggcgtgtc gaccgccgtc cgattggtgc gggtggccac
     3541 cctgaccgac gccatcgacg cgctgaccgc gctgaaggat cctgcccgcg cggccggcgt
     3601 tcgggggtgc gaatgagtac ctcggacgat ctggtggccg gcctgctcga gatcgagcat
     3661 cacgtgtcgg gactgggctg ggaccagccg gcacgactgt tcgccctggt acccaccacc
     3721 gagttgatcg ccgccgagcc gcaactggcg cagcacctga ccggcggcac ggcgcctcgg
     3781 cccgggcacc tgtccgccgt cgagcaggag gggttcgccg gtggcgacga tctgggcgag
     3841 gcgctcgccc ggatcagctg gccggccacg gtggccgggg tcgccttgag cctggagcgc
     3901 ctctttctgc ccggctcggc cgacaccgac ctgccccccg gtccgacggg cgacgagcag
     3961 gcccgccgcc accccgcccg tgaagagatc cgagtggtgg tgggtgcgtt gcgcgggggc
     4021 gattcctacg gtgtggcccg cattcgcagc catccggacg aactgttgtc gggtagtgac
     4081 ctggtgccgg ggctggctgc tgcactagca cgtacattgg actgacaatc cctactgagg
     4141 agcccttcgc gtgagcctgt ccgccagcgt ccaccggcga tccccgatac tgctgaccct
     4201 gggtatcgcg gccgtcgcga tcatcgcctt cgccgtgttc acccaggtgt ggaccgacaa
     4261 gctgtggttc gagtcgatcg gcttcggcac cgtgttcacc accgaactgc tgacccggct
     4321 ggggttgttc ttcggcgtcg cgatcgtcat ggcgctgctg atcggcggca acatgtggct
     4381 ggcgatccgg ctgcgtccac ggggacggcg cgccggtgac agcgcggtac tggacgcata
     4441 ccgccggctg ctcgagtcga acgcacgcat cgcggtgctc ggcccggcgc tggcgttcgg
     4501 gctgctggcc ggcatgtccg ccaccaccga ggtgctgccg ctgctggcct ggatcaacgc
     4561 ggtgccgttc ggccggaccg acccggtgtt cggcctcgac tattccttct acgtcttcga
     4621 ttttccggtc tggcagagcc tgctgtcgtt tctgctcacc gcgctgatct tcagcctgat
     4681 cgccgcagcc ctggtgcact tcgccatggg tggcatccgg ttcggacgta gcccgggccg
     4741 tccgcagttc agccaggccg cgcgggtgca cctgtcggtg ctgaccgggg tgctgctggt
     4801 gctgtacggg ctgcagtgcc tgctcgatcg ctacggcatg ctgctcagcc agggcaccct
     4861 gttcaccggc gtgcactaca ccgacgatca tgcgcgcatc ggcgccaagc tggtgatggc
     4921 cgtgatctcg ttcctggtgg cggggctgtt cttcgccaat ccgatcctca agcgcggcat
     4981 ctacccggcg gcggcgctgg tgctgatggt gatcagcggg ctgatcctca ccctgatcta
     5041 cccggcgatc gtgcagtcgt tcgtggtcgc gcccaaccag cccgacaaag agaaccccta
     5101 catgcagtcg cacatcaaca tgacccgcga cgcattcgga atcgccgaca ccgaggtgac
     5161 cgactacgag gccgtcaccc aggtgaaggc cggccagctc aaggccgacg ccgaggcact
     5221 gcccggcatt cgcctgatgg accccgcggt gatcggcccc acctacgagc agctgcagca
     5281 ggtgcgcggc tactacacct ttcccgacgt gctcgacgtc gaccgctaca ccatcgacgg
     5341 caa
//
LOCUS       DAOSUG010000171         5319 bp    DNA     linear   ENV 10-JUN-2024
DEFINITION  MAG TPA_asm: Micropruina sp. isolate AMR_MDS_2195
            NODE_6930_length_5319_cov_5.013298, whole genome shotgun sequence.
ACCESSION   DAOSUG010000171 DAOSUG010000000
VERSION     DAOSUG010000171.1
DBLINK      BioProject: PRJNA925477
            BioSample: SAMN32864262
            Sequence Read Archive: SRR4236673
KEYWORDS    WGS; ENV; Metagenome Assembled Genome; MAG; Third Party Data; TPA;
            TPA:assembly.
SOURCE      Micropruina sp. (wastewater metagenome)
  ORGANISM  Micropruina sp.
            Bacteria; Bacillati; Actinomycetota; Actinomycetes;
            Propionibacteriales; Nocardioidaceae; Micropruina.
REFERENCE   1  (bases 1 to 5319)
  AUTHORS   Abdulkadir,N., Saraiva,J.P., Zhang,J., Stolte,S., Gillor,O.,
            Harms,H. and Rocha,U.
  TITLE     Genome-centric analyses of 165 metagenomes show that mobile genetic
            elements are crucial for the transmission of antimicrobial
            resistance genes to pathogens in activated sludge and wastewater
  JOURNAL   Microbiol Spectr 12 (3), e0291823 (2024)
   PUBMED   38289113
REFERENCE   2  (bases 1 to 5319)
  AUTHORS   Abdulkadir,N.
  TITLE     Direct Submission
  JOURNAL   Submitted (24-MAR-2023) Environmental Microbiology, Helmholtz
            Center for Environmental Research -UFZ, Permoserstr 15, Leipzig
            04318, Germany
COMMENT     The annotation was added by the NCBI Prokaryotic Genome Annotation
            Pipeline (PGAP). Information about PGAP can be found here:
            https://www.ncbi.nlm.nih.gov/genome/annotation_prok/
            
            ##Genome-Assembly-Data-START##
            Assembly Method        :: SPAdes v. 3.11.1
            Genome Representation  :: Full
            Expected Final Version :: Yes
            Genome Coverage        :: Not Applicable
            Sequencing Technology  :: Illumina MiSeq
            ##Genome-Assembly-Data-END##
            
            ##Genome-Annotation-Data-START##
            Annotation Provider               :: NCBI
            Annotation Date                   :: 05/04/2023 04:57:10
            Annotation Pipeline               :: NCBI Prokaryotic Genome
                                                 Annotation Pipeline (PGAP)
            Annotation Method                 :: Best-placed reference protein
                                                 set; GeneMarkS-2+
            Annotation Software revision      :: 6.5
            Features Annotated                :: Gene; CDS; rRNA; tRNA; ncRNA
            Genes (total)                     :: 2,776
            CDSs (total)                      :: 2,750
            Genes (coding)                    :: 2,714
            CDSs (with protein)               :: 2,714
            Genes (RNA)                       :: 26
            tRNAs                             :: 24
            ncRNAs                            :: 2
            Pseudo Genes (total)              :: 36
            CDSs (without protein)            :: 36
            Pseudo Genes (ambiguous residues) :: 0 of 36
            Pseudo Genes (frameshifted)       :: 2 of 36
            Pseudo Genes (incomplete)         :: 32 of 36
            Pseudo Genes (internal stop)      :: 2 of 36
            ##Genome-Annotation-Data-END##
FEATURES             Location/Qualifiers
     source          1..5319
                     /organism="Micropruina sp."
                     /mol_type="genomic DNA"
                     /submitter_seqid="NODE_6930_length_5319_cov_5.013298"
                     /isolate="AMR_MDS_2195"
                     /isolation_source="Activated sludge of municipal
                     wastewater treatment plants"
                     /db_xref="taxon:2737536"
                     /environmental_sample
                     /geo_loc_name="Canada"
                     /lat_lon="49.25 N 123.1 W"
                     /collection_date="2015-06-16"
                     /metagenome_source="wastewater metagenome"
                     /note="metagenomic"
     gene            <1..764
                     /locus_tag="PLF56_06835"
     CDS             <1..764
                     /locus_tag="PLF56_06835"
                     /inference="COORDINATES: ab initio
                     prediction:GeneMarkS-2+"
                     /note="Derived by automated computational analysis using
                     gene prediction method: GeneMarkS-2+."
                     /codon_start=3
                     /transl_table=11
                     /product="peptidoglycan-binding protein LysM"
                     /protein_id="HOQ53314.1"
                     /translation="SIDEPPAAQPEHPYLQLFGPIELAGAAGPAPARSHRQCVEYCAW
                     LLENPNSTATRMTNELVVAETTRRSNMSRLRSWLGLDRDGRAYLPEAYSGRISLHPEV
                     SSDWQRMQVLTAPGLNRLTAETLVDVLELVRGAPLADAAPGQWRWAEELRTDMSSVAR
                     DAGVLLARWAVERRDLDLARWAAARALVAAPEDELLLVERIHTEHAAGNTGEVERLVR
                     WVTSQARVLGVDLAPETVDTCQRVLEGRTRPRAVR"
     gene            1496..2128
                     /gene="pdxH"
                     /locus_tag="PLF56_06840"
     CDS             1496..2128
                     /gene="pdxH"
                     /locus_tag="PLF56_06840"
                     /EC_number="1.4.3.5"
                     /inference="COORDINATES: protein motif:HMM:NF004231.0"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_function: GO:0004733 - pyridoxamine-phosphate oxidase
                     activity [Evidence IEA];
                     GO_function: GO:0010181 - FMN binding [Evidence IEA];
                     GO_process: GO:0008615 - pyridoxine biosynthetic process
                     [Evidence IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="pyridoxamine 5'-phosphate oxidase"
                     /protein_id="HOQ53315.1"
                     /translation="MDPAGMRLNYDLDSLSEEAASDEPLHQFDAWFAQAAAGDVLEAN
                     AMTLATVGPDGRPSTRIVLLKGYDAAGLVFFTNYESRKGRDLAGQPFAALQFYWPSLQ
                     RVVRVEGPVEQVSAAESDEYFASRPLGSRLGAWASPQSEVIADREVLVEAEAEASAHF
                     GEHPPRPPHWGGYRLAPEVWEFWQGRPSRLHDRLRYRLDDGRWVRERLAP"
     gene            complement(2266..3141)
                     /locus_tag="PLF56_06845"
     CDS             complement(2266..3141)
                     /locus_tag="PLF56_06845"
                     /inference="COORDINATES: protein motif:HMM:NF012791.2"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_function: GO:0032559 - adenyl ribonucleotide binding
                     [Evidence IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="universal stress protein"
                     /protein_id="HOQ53316.1"
                     /translation="MTYLVGYSPHKDDSCALELACQFARSEAGTVHALTVVPRGWESV
                     DGSATDGEFERWAAEEGEASAAEALAQLSRHSDVTSSATWVTGRSVPAAMLQQATELG
                     ASLIVVGSGTDGVPGRIALTSKTDRLLHSSHVPVAIPPRGYSPEPGSRITRVTLAFRD
                     DDATWRLLDEVADICRQIDAELRLLTVAIKQRPMVTSGVRRNEKLVFANLLRQVAAAQ
                     AEAIDHLTAAGVKAATVTAEGASWTDALATVAFDGGDVLVVGSSSTHRLAHVFLGSSA
                     TKILRNSPVPVVVVP"
     gene            complement(3138..4649)
                     /locus_tag="PLF56_06850"
     CDS             complement(3138..4649)
                     /locus_tag="PLF56_06850"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_013864076.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_component: GO:0016020 - membrane [Evidence IEA];
                     GO_function: GO:0022857 - transmembrane transporter
                     activity [Evidence IEA];
                     GO_process: GO:0055085 - transmembrane transport [Evidence
                     IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="amino acid permease"
                     /protein_id="HOQ53317.1"
                     /translation="MALTLREQLLRRKPIVFQQQHHQGHELARTLSTFQLMMFGVGAT
                     VGTGIFFVLGEAVPKAGPAVLISFLIAGIAAGLSALCYAELASAIPVSGSTYSYAYHA
                     LGELVAVVIAGCVLLEYGVSTGAVAVGWSSYFNELLQETIGWHLPDALSVSPIPVPVE
                     DALGVITYPATGGLVNLPAMVLVGLCALLLIRGASESATVNAIMVMIKLGVLVLFTVI
                     GFSAFNADHFANFFGYGIGGISAAAGTIFFSFIGLDAVATAGEEVKNPQKALPRAIIG
                     ALAIVSGIYIAVAAAGLAAKPVEFFKSTQGEAGLALILKEVTGNQLWSTVLAAGAVIS
                     IFSVTLVTLYGQTRILFAVGRDGLIPKKFLEVNPKTMTPTYNTIVVSIIVALIGGFVP
                     AVYLWDTVSIGTLFAFSVVAIGIIVLRQTHPGLERPFKVPGYPVVPALTVLVCLYIMW
                     GLAWITWVIFACWIAVVLAFYFSYGRRHATLNHYVDEEEIAEPGGRKRRDDEQ"
     gene            complement(4889..>5319)
                     /locus_tag="PLF56_06855"
     CDS             complement(4889..>5319)
                     /locus_tag="PLF56_06855"
                     /inference="COORDINATES: protein motif:HMM:NF014822.2"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_function: GO:0003824 - catalytic activity [Evidence
                     IEA];
                     GO_function: GO:0043169 - cation binding [Evidence IEA];
                     GO_process: GO:0005975 - carbohydrate metabolic process
                     [Evidence IEA]"
                     /codon_start=3
                     /transl_table=11
                     /product="alpha amylase C-terminal domain-containing
                     protein"
                     /protein_id="HOQ53318.1"
                     /translation="QIAAAQGRGGNGIVRLYRDLIQLRRDYHSTTKGLKGSGLRIIRT
                     DDEKNLLAFQRWYDHGPRDDVVVIANLDAQAKQKYRIGMPETGLWKLRFNSDATNYSD
                     AFGDFDSFDVTAYEGECDGLGAHAEIDIAPYSLLIYSMDE"
BASE COUNT          852 a         1798 c         1818 g          851 t
ORIGIN      
        1 tcagcatcga cgagccgccg gctgcgcaac ccgagcaccc ctacctgcag ttgttcggac
       61 cgatcgaact ggccggcgcc gccggcccgg cccccgcccg ctcgcaccgc cagtgtgtgg
      121 agtactgcgc ctggctgctc gagaacccca acagcacggc cacccgcatg accaacgaac
      181 tggtggtggc cgagaccacc cggcgatcca acatgtcgcg gttgcgctcg tggctcggct
      241 tggatcgcga cggccgcgcc tacctgccgg aggcctactc cggacgcatc tcgctgcatc
      301 cggaagtctc gtccgactgg cagcgcatgc aggtgctgac cgcgccgggc ctcaatcgtc
      361 tgaccgccga aacgctggtg gacgtgctcg aactggtccg cggcgcaccg ctggccgacg
      421 ccgccccggg ccagtggcgc tgggcggagg agttgcgcac cgacatgtcg tccgttgccc
      481 gtgacgccgg tgtgctgctc gcccggtggg cggtcgaacg ccgtgatctc gatttggcgc
      541 gttgggccgc ggcgcgggca ctggtggcgg cacccgagga cgagttgctg ctggtggagc
      601 gcattcatac cgagcacgca gccggcaaca ccggcgaggt ggagcggctg gtgcgctggg
      661 tgaccagtca ggcgcgagtg ctcggcgtcg acctggcccc tgagactgtt gacacctgtc
      721 agcgcgtgct tgagggacga acccggccac gggccgtccg ctgagtgggt gggcctggtc
      781 cggttgccgt ccggattgag cccggctcga ccgatccccg cctggcgtgc agcctcatcc
      841 gggccgtgcc ggccgccctc acggacgccc ggtgtgtccc cgcgcgaccg tgggttcgcc
      901 ggcttgggtg aaccttgcct cgagtgagtt cccgcacgat cctcggtgag ccggcttggg
      961 ttgatccttc ctcgagtgtg ttcctgcgcg atcgtgggtg agccgacttg ggtggacctt
     1021 tcctctagcg ggtgggtttt ggtacagcag tcggtgctga aaccccgtag cgcctcgggc
     1081 gtgtctcctc accctgggcg ttccgcgagg acgtgcccag tgcgtctgat cacaaggcgg
     1141 aagacgagtc gatagcgggc cctctcgccg aggatgacaa cgcagcgagc gggcgtactg
     1201 ggtgcaccgc agctgcggcc agggtgagga gacgcgccct agtcaccggg gcccccagct
     1261 gaggtgccca tccgggaacc ctggctgcgc cggccgcgct caacaaccgc ccggtatgtg
     1321 ccccacggat tcgcctgccg tcctacccgt cagatcccgc gaggtaccgc ttcggtgagt
     1381 gcctgcacac ggcgaaccgg atcagcgagc gggccatcgg tggatccggg ctgactccgc
     1441 cgtggtgcgc actgaacaga cctccgagat cgagcgggcg tcctaagctc gtcgcatgga
     1501 ccctgccggc atgcgcctca actacgacct cgacagcctc agcgaagagg cggcgtccga
     1561 tgagccgctg caccagttcg atgcgtggtt cgcccaagcc gcggcgggtg acgtgctcga
     1621 ggccaacgcc atgacgctgg ccaccgtggg gccggacgga cgcccgtcca cccgcatcgt
     1681 gctgctcaag ggctacgacg cggccgggct ggtgttcttc accaactacg aatcccgcaa
     1741 gggccgcgac ctggcgggcc agccgttcgc ggcactgcag ttctactggc cgagcctgca
     1801 gcgggtggtg cgggtcgagg gtccggtcga gcaggtcagc gccgccgagt cggacgagta
     1861 tttcgccagc cgtccgctcg gctcacgact gggcgcctgg gcgtcaccgc agagcgaggt
     1921 gatcgccgat cgtgaggtgc tggtcgaagc cgaggccgag gcgtccgcac acttcggcga
     1981 gcacccgccg cgtcccccgc actggggtgg ttaccggctc gcacccgagg tgtgggagtt
     2041 ctggcagggc cgcccgtcgc ggctgcacga ccgcctgcgt tatcgcctgg acgacggccg
     2101 ctgggtgcgc gaacggctcg cgccctgagg ggccggtccg ctgcccgatc cgttgggtga
     2161 gcgggtcaaa acccctagcc gcatggtctc gaccagctcg accagctcga ccagcgggcc
     2221 tttcgctcga ccagcgaagt ctcgacaagc tcgactagcg acggatcagg gcaccaccac
     2281 caccggaacc ggggagttgc gcaggatctt ggttgcgctg gagcccagaa acacgtgcgc
     2341 cagccgatgg gtggaggacg acccgaccac cagcacgtca ccgccgtcga acgcaacggt
     2401 ggccagcgcg tccgtccacg aggcgccttc ggcggtgacg gtggccgcct tgacgccggc
     2461 ggcggtgaga tggtcgatcg cttcggcttg cgccgccgcc acctgtcgca acaggttggc
     2521 gaagaccagt ttctcgttgc gccgcacacc cgaggtgacc atcggacgct gcttgatcgc
     2581 gacggtgagc agccgcaact cggcatcgat ctgcctgcag atgtcggcca cctcgtcgag
     2641 caggcgccag gtggcgtcgt cgtcacggaa tgccagcgtg acccgcgtga ttctcgaccc
     2701 cggttcggga ctgtagccgc gcggcgggat cgccaccggc acgtgcgaac tgtgcagtag
     2761 gcggtccgtc ttcgacgtaa gtgcgatgcg tcccggtacc ccgtcggtgc cggagccgac
     2821 cacgatcagg gacgccccca actccgtcgc ctgctgcagc atggctgcgg gcacggagcg
     2881 tccggtcacc caggtggcgc tcgaggtcac atcggaatgc ctggagagct gcgccaacgc
     2941 ctccgcggcg ctcgcctcgc cttcttcggc ggcccatctc tcgaactcgc cgtcggtcgc
     3001 actgccgtcg acgctctccc agccgcgcgg cacgacggtc agggcatgga cggtgccggc
     3061 ctccgagcgg gcgaactggc aggccagttc gagggcgcaa ctgtcgtcct tgtgcgggct
     3121 gtacccgacc aggtaggtca ttgttcgtcg tcccgtcgct tgcggccgcc cggttcggcg
     3181 atctcttctt catcgacgta gtggttgagg gtggcgtgcc ggcgtccgta gctgaagtag
     3241 aacgccaaca ccacggcgat ccagcaggcg aagatcaccc acgtgatcca ggccagaccc
     3301 cacatgatgt agagacagac cagcacggtg agggctggca ccaccgggta gccgggcacc
     3361 ttgaacggac gctccaggcc cgggtgggtt tgtctcagta cgatgatgcc gatcgccacc
     3421 accgagaagg cgaacagggt gccgatcgag accgtgtccc acagatagac ggccggcacg
     3481 aacccgccga tcaacgcaac gatgatcgac accacgatgg tgttgtaggt gggcgtcatg
     3541 gtcttcgggt tgacctcgag aaacttcttc ggaatgagcc cgtcccgtcc caccgcgaac
     3601 aggatgcggg tctggccgta cagggtgacc agggtgaccg agaagatcga gatcaccgcg
     3661 ccggcggcca acacggtgct ccacaactgg ttgccggtca cctccttcag gatcagcgcc
     3721 agcccggcct cgccctgggt ggatttgaag aactccaccg gcttcgcggc gaggccggct
     3781 gcggcgacgg cgatgtagat gccggagacg atggccagcg ccccgatgat tgcgcgcggc
     3841 aacgccttct gcgggttctt cacctcttca ccggcggtcg ccaccgcgtc caggccgatg
     3901 aaactgaaga agatggtgcc ggccgcggca ctgatgccgc cgatgccgta gccgaagaag
     3961 ttggcgaagt ggtcggcgtt gaaggcgctg aagccgatca cggtgaagag caccagcacg
     4021 cccagcttga tcatgaccat gatcgcgttg acggttgccg actcggacgc gccgcggatc
     4081 agcagcaacg cacacaggcc gaccagcacc atcgcgggca ggttcaccag gccgccggtg
     4141 gccggatagg tgatcacgcc cagtgcgtcc tccacgggca cggggatggg tgacaccgac
     4201 agggcgtccg gcaggtgcca gccgatcgtc tcttgcagca gttcgttgaa atagctgctc
     4261 cagccgaccg ccaccgctcc ggtcgacacc ccgtactcca gcaacacgca gcccgcgatg
     4321 accaccgcca ccagttcgcc gagtgcgtgg taggcgtagc tgtaggtcga cccgctgacc
     4381 ggtatcgcgg acgccagttc ggcatagcac aatgccgaca ggccggccgc gatgccggcg
     4441 atgagaaacg agatcagcac cgcgggcccg gccttgggta ccgcctcgcc gagcacgaag
     4501 aagatgccgg tgccgaccgt ggcaccgacg ccgaacatca tcagttggaa ggtgctcagg
     4561 gttcgggcca gctcatggcc ctgatggtgc tgctgctgaa agacgatcgg cttgcgccgc
     4621 agcaactgct cgcgaagagt caaggccatg atcggctccc gggggatcgg cgaagggtgc
     4681 gcacaagcta gcgcgctgca agggtcggtg ggaacccctg cggcgcaggc cagagccgat
     4741 gcgctgggca ccgttgccac ccgtcgccgg cacaccgggg cgcagtcgtc accccacatc
     4801 gcgcggtcaa gagttcgttg cgcgtggacg tacccgcgca acgagcgcaa caccgcgcaa
     4861 cgtcggttga cggacggggc ggcgtgccct actcgtccat gctgtagatc agcaggctgt
     4921 acggcgcgat gtcgatctcg gcgtgcgctc cgagcccgtc gcactcgccc tcatacgcgg
     4981 tgacgtcgaa cgagtcgaag tcaccgaagg cgtcggagta gttggtggcg tcgctgttga
     5041 accgcagctt ccacagcccg gtctcgggca tgccgatgcg gtacttctgc ttggcctgag
     5101 cgtccaggtt ggcgatcacc accacgtcgt cgcgcgggcc gtggtcgtac cagcgctgga
     5161 acgccagcag gttcttctcg tcgtcggtgc ggatgatgcg cagccccgaa cccttcagtc
     5221 ccttggtggt cgagtggtag tcgcggcgaa gttggatcag gtcgcggtac agccggacga
     5281 tgccgttgcc cccgcgtccc tgggccgccg cgatttggg
//
LOCUS       DAOSUG010000172         5319 bp    DNA     linear   ENV 10-JUN-2024
DEFINITION  MAG TPA_asm: Micropruina sp. isolate AMR_MDS_2195
            NODE_6931_length_5319_cov_3.495441, whole genome shotgun sequence.
ACCESSION   DAOSUG010000172 DAOSUG010000000
VERSION     DAOSUG010000172.1
DBLINK      BioProject: PRJNA925477
            BioSample: SAMN32864262
            Sequence Read Archive: SRR4236673
KEYWORDS    WGS; ENV; Metagenome Assembled Genome; MAG; Third Party Data; TPA;
            TPA:assembly.
SOURCE      Micropruina sp. (wastewater metagenome)
  ORGANISM  Micropruina sp.
            Bacteria; Bacillati; Actinomycetota; Actinomycetes;
            Propionibacteriales; Nocardioidaceae; Micropruina.
REFERENCE   1  (bases 1 to 5319)
  AUTHORS   Abdulkadir,N., Saraiva,J.P., Zhang,J., Stolte,S., Gillor,O.,
            Harms,H. and Rocha,U.
  TITLE     Genome-centric analyses of 165 metagenomes show that mobile genetic
            elements are crucial for the transmission of antimicrobial
            resistance genes to pathogens in activated sludge and wastewater
  JOURNAL   Microbiol Spectr 12 (3), e0291823 (2024)
   PUBMED   38289113
REFERENCE   2  (bases 1 to 5319)
  AUTHORS   Abdulkadir,N.
  TITLE     Direct Submission
  JOURNAL   Submitted (24-MAR-2023) Environmental Microbiology, Helmholtz
            Center for Environmental Research -UFZ, Permoserstr 15, Leipzig
            04318, Germany
COMMENT     The annotation was added by the NCBI Prokaryotic Genome Annotation
            Pipeline (PGAP). Information about PGAP can be found here:
            https://www.ncbi.nlm.nih.gov/genome/annotation_prok/
            
            ##Genome-Assembly-Data-START##
            Assembly Method        :: SPAdes v. 3.11.1
            Genome Representation  :: Full
            Expected Final Version :: Yes
            Genome Coverage        :: Not Applicable
            Sequencing Technology  :: Illumina MiSeq
            ##Genome-Assembly-Data-END##
            
            ##Genome-Annotation-Data-START##
            Annotation Provider               :: NCBI
            Annotation Date                   :: 05/04/2023 04:57:10
            Annotation Pipeline               :: NCBI Prokaryotic Genome
                                                 Annotation Pipeline (PGAP)
            Annotation Method                 :: Best-placed reference protein
                                                 set; GeneMarkS-2+
            Annotation Software revision      :: 6.5
            Features Annotated                :: Gene; CDS; rRNA; tRNA; ncRNA
            Genes (total)                     :: 2,776
            CDSs (total)                      :: 2,750
            Genes (coding)                    :: 2,714
            CDSs (with protein)               :: 2,714
            Genes (RNA)                       :: 26
            tRNAs                             :: 24
            ncRNAs                            :: 2
            Pseudo Genes (total)              :: 36
            CDSs (without protein)            :: 36
            Pseudo Genes (ambiguous residues) :: 0 of 36
            Pseudo Genes (frameshifted)       :: 2 of 36
            Pseudo Genes (incomplete)         :: 32 of 36
            Pseudo Genes (internal stop)      :: 2 of 36
            ##Genome-Annotation-Data-END##
FEATURES             Location/Qualifiers
     source          1..5319
                     /organism="Micropruina sp."
                     /mol_type="genomic DNA"
                     /submitter_seqid="NODE_6931_length_5319_cov_3.495441"
                     /isolate="AMR_MDS_2195"
                     /isolation_source="Activated sludge of municipal
                     wastewater treatment plants"
                     /db_xref="taxon:2737536"
                     /environmental_sample
                     /geo_loc_name="Canada"
                     /lat_lon="49.25 N 123.1 W"
                     /collection_date="2015-06-16"
                     /metagenome_source="wastewater metagenome"
                     /note="metagenomic"
     gene            <1..452
                     /locus_tag="PLF56_06860"
     CDS             <1..452
                     /locus_tag="PLF56_06860"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_011754710.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=3
                     /transl_table=11
                     /product="carboxyl transferase domain-containing protein"
                     /protein_id="HOQ53319.1"
                     /translation="PGVAQEHGGIIRHGAKMLYAYAAATVPKITVVLRKAYGGAYIAM
                     SAKDLGADQVFAWPTAEIAVMGAEGAASVVFKREIDAADDPVTRRAELVEEYRDTFST
                     PFVAASRGLVDDVIDPGDTRRKVAMALELLTAKRQLRPAKKHGLGPT"
     gene            466..732
                     /locus_tag="PLF56_06865"
     CDS             466..732
                     /locus_tag="PLF56_06865"
                     /inference="COORDINATES: ab initio
                     prediction:GeneMarkS-2+"
                     /note="Derived by automated computational analysis using
                     gene prediction method: GeneMarkS-2+."
                     /codon_start=1
                     /transl_table=11
                     /product="hypothetical protein"
                     /protein_id="HOQ53320.1"
                     /translation="MSDQDAELLEMVKALTDKVTAMEVEIKQLRLLNADVPEETMVAI
                     AAAVAAYLGHRAKRRVAHFTTSAIWQSGTRRRQHAHEPLHLRKA"
     gene            741..1100
                     /locus_tag="PLF56_06870"
     CDS             741..1100
                     /locus_tag="PLF56_06870"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_011754712.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="biotin/lipoyl-binding protein"
                     /protein_id="HOQ53321.1"
                     /translation="MKLKVTVNGIAYDIEVEVEEQERPSLGSVIIGGGGSSSAPTTAS
                     VKAASANAVVAPLSGAVARILVAEGDEITEGQVLLVLEAMKMETEITAPHDGTVAAVL
                     VKERDSVAGGEALIELA"
     gene            1129..1332
                     /locus_tag="PLF56_06875"
     CDS             1129..1332
                     /locus_tag="PLF56_06875"
                     /inference="COORDINATES: ab initio
                     prediction:GeneMarkS-2+"
                     /note="Derived by automated computational analysis using
                     gene prediction method: GeneMarkS-2+."
                     /codon_start=1
                     /transl_table=11
                     /product="hypothetical protein"
                     /protein_id="HOQ53322.1"
                     /translation="MPKPQWGWFAVAVIAMVTGVFVVAMGFGDFAAKSGPPGGVVVAV
                     LGLVTIWSFGKAFFNFPDYRNRQ"
     gene            complement(1465..2742)
                     /locus_tag="PLF56_06880"
     CDS             complement(1465..2742)
                     /locus_tag="PLF56_06880"
                     /inference="COORDINATES: ab initio
                     prediction:GeneMarkS-2+"
                     /note="Derived by automated computational analysis using
                     gene prediction method: GeneMarkS-2+."
                     /codon_start=1
                     /transl_table=11
                     /product="hypothetical protein"
                     /protein_id="HOQ53323.1"
                     /translation="MRVLRALALLVVVVSIAAAAHVVHAFTQPLSDTARVAAQVRWLN
                     AARADGAAQQMQELFPEGEFFTHLLTGLAAASTGDIDTVRSALAASGTPAVRDRFGDI
                     DELAGGTFYRGWRLLLWAEQVRLSGADGAALLAEADAVQAALLANPRGVPASYPGGYW
                     PCDAVVAMAAVVRARALGGDPIAEADLAAWRDRVSPLREPSTGLLPHRVSAAGVAEGP
                     RGSSQAIIQAFWPDVDPAGAADQWQRFVATFVAREAGLVGVREHPRGTDAAGDVDSGP
                     LLFGVSASASAVSLAAALRNGDSALALELDRQAEYLGLPLEWAGQRRFVFGLVPVGDA
                     FVAWARSVPVAPASVGSAAPAALWWVHALVLLVPGLLAGVLLVRTRRPVPQRHATGRP
                     ASPAYEIPGQARDDEHDPNPTTPDVRDLGSDPR"
     gene            complement(2739..3482)
                     /locus_tag="PLF56_06885"
     CDS             complement(2739..3482)
                     /locus_tag="PLF56_06885"
                     /EC_number="1.-.-.-"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_002528959.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_function: GO:0016491 - oxidoreductase activity
                     [Evidence IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="SDR family oxidoreductase"
                     /protein_id="HOQ53324.1"
                     /translation="MSSKRAVVTGASSGIGAASARALAAAGYQVICAARRTDRIEALA
                     AEIGGVAVTCDVTRDDAVADLAEAVGDRLDLLVNNAGGALGQNPVAQAEIDAWQRMYD
                     INVLGAGRVTRALLPALIAGEGMVLFVTSTAAEAAYEGGAGYCGVKAAERSLAGSLRL
                     ELFDQPVRVCEISPGMVHTEEFSTTRFGGDQAKADAVYAGVPGPLSAEDVADAICWMA
                     TRPAHVNVDRLVIRPRAQAANHKVYRVTA"
     gene            3593..4345
                     /locus_tag="PLF56_06890"
     CDS             3593..4345
                     /locus_tag="PLF56_06890"
                     /EC_number="5.4.2.11"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_004810615.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_function: GO:0004619 - phosphoglycerate mutase activity
                     [Evidence IEA];
                     GO_process: GO:0006096 - glycolytic process [Evidence
                     IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="phosphoglyceromutase"
                     /protein_id="HOQ53325.1"
                     /translation="MSANLILLRHGESEWNALNLFTGWVDVDINEKGVGEAERGGHLL
                     TEAGLLPDVLHTSVLRRAIHTANLALDACDRHWIPVRRHWRLNERHYGDLQGKNKAET
                     LEEYGEEKFMLWRRSYDTPPPPISDDNPNTQFDDPRYAFLPTEARPKTECLKDVVIRM
                     LPYWYDAIVPDLRAGHTVLVTAHGNSLRALVKHLDAISDAEIAGLNIPTGIPLHYELD
                     DDLQPVTKGGTYLDPAAAAAAIEAVKNQGKKK"
     gene            complement(4832..>5319)
                     /locus_tag="PLF56_06895"
     CDS             complement(4832..>5319)
                     /locus_tag="PLF56_06895"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_002528948.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=3
                     /transl_table=11
                     /product="PhoU domain-containing protein"
                     /protein_id="HOQ53326.1"
                     /translation="NRCFVLLARQAPVAGELRTIVAAMRMVADLARMGDLACHVAKIA
                     RLRYPEKAVPEGLVPNFELMARVAEDMVSTAGRALNDRNAHDAAKLADSDEEMDELRK
                     SQFRILLGSDWPYSVESAVDVALLGRYYERIADHAVIMAGRVIYFVTGTHPAGENWTY
                     A"
BASE COUNT          853 a         1909 c         1785 g          772 t
ORIGIN      
        1 tgcccggcgt ggcgcaggag cacggcggca tcatccggca cggcgccaag atgctgtacg
       61 cctatgcggc ggcgacggtg ccgaagatca ccgtggtgct gcgcaaggcc tatggcggtg
      121 cgtacatcgc gatgtccgcc aaggatctgg gtgccgatca ggtgttcgcc tggccgaccg
      181 ccgagatcgc cgtgatgggt gccgaaggtg cggcatcggt ggtgttcaag cgtgagatcg
      241 acgcggccga cgatccggtc accaggcgcg ccgaactcgt cgaagagtac cgcgacacgt
      301 tctcgacccc gttcgtggcc gcgtcccgtg gcctggtgga cgacgtgatc gacccgggcg
      361 acacccgtcg caaggtggcc atggcgctgg agttgctgac cgccaaacgt cagctgcggc
      421 cggccaagaa gcacggcctc ggcccgacgt gatcggagag atgcgatgag cgaccaagac
      481 gccgaactgc tcgagatggt caaggcgctg accgacaagg tcaccgccat ggaggtcgag
      541 atcaagcagt tgcgcctgct gaacgccgac gtgcccgaag agacgatggt ggccattgcg
      601 gccgccgtcg ccgcctacct cgggcaccgg gccaagcggc gcgtagccca cttcaccaca
      661 tccgccatct ggcagagcgg cacccgccgc cggcagcacg cccacgagcc gctgcacctg
      721 cgcaaagcct gaggaaagcc atgaaactga aggtcactgt caacggcatc gcctacgaca
      781 tcgaagtgga ggtcgaagag caggagcgtc ccagcctcgg ctcggtcatc atcggtggtg
      841 gcggctcgtc gtcggctccc accacggcgt cggtgaaagc cgcgtccgcg aacgcggtcg
      901 tcgcgcccct gtcgggcgcg gtcgcccgca ttctggtcgc cgagggcgac gagatcaccg
      961 aaggccaggt gctgctggtg ctcgaagcca tgaagatgga aaccgagatc accgcgccgc
     1021 acgacggcac ggtggcggcg gtgctggtca aggagcgcga ttcggtcgcg ggcggcgagg
     1081 cactcatcga gctagcctga gtcggaacgt tcgatctcag gaggcacgat gccgaaaccc
     1141 caatgggggt ggttcgcagt ggccgtcatc gccatggtca ccggcgtgtt cgtggtggcc
     1201 atggggttcg gcgacttcgc cgcaaagagt ggccctccgg gcggagtcgt cgtcgccgtt
     1261 ctcggcctcg tcacgatttg gagcttcggc aaggccttct tcaactttcc cgactaccgc
     1321 aaccggcagt agcaggttgg gggtcatact cgtcatcccg ggccacggga tctctcaagc
     1381 cgggggtggt tgggttgggg tcatgctcgt catctcgggt ctgacccggg atctctcacg
     1441 ccgggggtgg ttgggtcatg ctcgtcatct cgggtctgac ccgagatctc tcacatcggg
     1501 agtagttggg ttggggtcat gctcgtcatc ccgggcttga cccgggatct cgtacgccgg
     1561 ggacgccgga cgcccggttg cgtgacgctg cggcactggg cgtctggttc tgaccaggag
     1621 cacccccgcg agtagtccgg gcaccagcag caccagggcg tgcacccacc acaacgcggc
     1681 cggagccgcc gagccgacgg acgccggcgc caccgggacg ctgcgcgccc aggccacgaa
     1741 cgcgtccccg accggcacca gtccgaagac gaaccggcgc tgcccggccc actccagcgg
     1801 cagccccagg tactcggcct ggcggtccaa ctcgagcgcg agagcgctgt cgccgttgcg
     1861 cagcgcggcg gccaggctga ccgccgaggc cgacgccgac accccgaaca gcaacggccc
     1921 ggaatcgacg tcaccggcgg cgtccgtccc acgcgggtgc tcgcggacgc cgaccagtcc
     1981 ggcctcgcgg gccacgaagg tggcgacgaa ccgctgccac tggtcagccg ccccggccgg
     2041 gtcgacgtcg ggccagaagg cctggatgat cgcctgcgag ctgcctcgcg gcccctcggc
     2101 gacgcccgca gccgacaccc ggtgcggcag cagcccggtc gagggctcgc gcaaggggct
     2161 cacccggtcg cgccatgccg ccaggtcggc ctccgcgatc gggtcaccgc ccaacgcccg
     2221 ggcgcgcacc accgccgcca tcgcgaccac ggcgtcgcag ggccagtacc cgcccgggta
     2281 cgacgccggg acgccgcggg ggttcgccag cagggcggcc tgcaccgcat ccgcttcggc
     2341 gagcagtgcg gcgccgtccg cgccgctcag ccgcacctgc tcggcccaca gcagcagccg
     2401 ccaaccgcgg tagaacgtgc cgccggcgag ctcgtcgatg tcaccgaacc ggtcgcgaac
     2461 cgccggcgtc ccgctcgccg ccagggccga gcgcaccgtg tcgatgtcgc cggtcgaggc
     2521 tgctgccaac ccggtcagca ggtgggtgaa gaactcgccc tcggggaaca gttcctgcat
     2581 ctgctgcgcg gctccatcgg cccgggcggc attcagccag cgcacctggg cggccaccct
     2641 cgcggtatcg ctgagcggct gggtgaacgc atgcaccaca tgtgccgcgg cggcgatgct
     2701 gaccaccacc accaacaagg ccagggcacg cagcaccctc atgcagtgac ccggtacacc
     2761 ttgtggttgg cggcctgggc gcgggggcgg atcaccaggc ggtcgacgtt gacgtgggcc
     2821 gggcgggtgg ccatccagca gatcgcgtcg gcgacgtcct cggcactcag cgggccgggc
     2881 accccggcgt agacggcgtc cgccttcgcc tggtcgccgc cgaaccgggt cgtggagaac
     2941 tcctcggtgt gcaccatgcc gggcgagatc tcgcacaccc ggacgggctg gtcgaacagt
     3001 tccagccgca gcgagccggc cagcgaccgt tcggcggcct tcaccccgca gtacccggcg
     3061 ccaccttcat aggcggcctc cgcggcggtc gaggtgacga acagcaccat gccttcgccg
     3121 gcgatcaggg cgggcagcag cgcccgggtg acccgcccgg caccgagcac gttgatgtcg
     3181 tacatccgtt gccaggcgtc gatctcggcc tgcgccaccg ggttctggcc gagcgcaccg
     3241 ccggcgttgt tgaccaacag gtcgagccgg tcgccgacag cctcggccag atcggccact
     3301 gcgtcgtcac gggtgacgtc gcaggtcacc gcgacgccac cgatctcggc cgccagcgcc
     3361 tcgatccggt cggtgcgccg ggcggcgcag atcacctggt agccggccgc agccagcgcc
     3421 ctggcactcg ccgccccgat cccgctgctc gctccggtga cgactgctcg tttgctgctc
     3481 atgcgcagat tgtgccagcc gcgcgggcac gtccggctcg gggagttcgg tctttgaccg
     3541 aatcccatgc agcacctgtc tggtcccccc gctcaccact aggctcggcc ccatgagtgc
     3601 gaacctgatc ctgctccgcc atggcgagag cgagtggaat gccctgaacc tgtttacggg
     3661 ctgggtcgat gtcgacatca acgagaaggg tgtcggcgaa gcggaacgcg gcggccatct
     3721 gctgaccgag gccgggctgc tgccggacgt gctgcacacc tcggtgctgc gtcgcgcgat
     3781 ccacaccgcg aacctggccc tcgacgcctg cgaccggcat tggattccgg ttcgccggca
     3841 ttggcgcctc aacgagcgtc attacggcga tctacagggc aagaacaaag ccgagacgct
     3901 ggaagagtac ggcgaagaga agttcatgct ctggcgccgc agctacgaca cacctccgcc
     3961 ccccatctcg gacgacaacc cgaacaccca gttcgacgat ccgcggtacg cgtttctgcc
     4021 caccgaggca cggcccaaga ccgagtgcct caaagacgtc gtgattcgca tgttgccgta
     4081 ctggtacgac gcgatcgtgc ccgacctgcg cgccggtcac accgtgctgg tgaccgcgca
     4141 cggcaactcg ctgcgcgccc tggtgaagca cctcgacgcc atctcggacg ccgagatcgc
     4201 cggactgaac attcccaccg gcattccgct gcactacgag ttggacgacg acctgcagcc
     4261 ggtcaccaag ggcggcacct acctcgatcc cgccgccgct gccgcggcca tcgaggcggt
     4321 caagaaccag ggcaagaaga agtagctccg cgacctgtca gcgccccgca cgccaggcga
     4381 gtgcggggcg ctgtcatgcc tctcgaacgc tcggtcgcaa cgccgagccc ccggtcgttc
     4441 gcatgcacag tcggccccca ggacggccgg cgacacggct cccagcctcc agatcaaacg
     4501 ccgagcatgc ccggtcgctc gcgagtggcg cctgacggcc gaacgcacgg cttcccgcct
     4561 ccagatcgca tgccgagccg gagggtcgct ctcgaatggc gccctagtcg cagggatatc
     4621 cggccctcca gccgtttcga cgtccatccg ctcgtccgag tcccagaggc cggtcgccca
     4681 gccgctccgc atgtccgagt ctcagaggcg cggtcgcaca gccgtccagc cgctccgcac
     4741 gtccgagccc cagcggcccg gtcgcccagc cgctccggac gtccgagtcc cagcggcgca
     4801 gtcgtctagc cgctcaggcg cccaagctga ctcaggcgta ggtccagttc tcaccagccg
     4861 gatgggtgcc ggtgacgaag tagatcaccc ggccggccat gatcaccgcg tggtcggcga
     4921 tgcgctcgta atagcggccc agcaacgcca cgtcgacggc cgactccacc gagtagggcc
     4981 aatccgagcc gagcagaatg cggaactggc tcttgcgcag ctcgtccatc tcttcgtcgc
     5041 tgtcggccag cttggccgcg tcgtgggcgt tgcggtcgtt gagcgcacgt ccagcggtcg
     5101 agaccatgtc ttcggccacc cgtgccatca actcgaagtt gggcacgagg ccttcgggca
     5161 ccgccttctc ggggtaccgt agccgagcga ttttcgccac gtgacaagcc aggtcgccca
     5221 tgcgtgccag gtcggccacc atgcgcatcg cggccacgat ggtgcgtagt tcgccggcca
     5281 ccggtgcctg gcgggcgagc agcacgaagc agcggttct
//
LOCUS       DAOSUG010000173         5315 bp    DNA     linear   ENV 10-JUN-2024
DEFINITION  MAG TPA_asm: Micropruina sp. isolate AMR_MDS_2195
            NODE_6936_length_5315_cov_4.240114, whole genome shotgun sequence.
ACCESSION   DAOSUG010000173 DAOSUG010000000
VERSION     DAOSUG010000173.1
DBLINK      BioProject: PRJNA925477
            BioSample: SAMN32864262
            Sequence Read Archive: SRR4236673
KEYWORDS    WGS; ENV; Metagenome Assembled Genome; MAG; Third Party Data; TPA;
            TPA:assembly.
SOURCE      Micropruina sp. (wastewater metagenome)
  ORGANISM  Micropruina sp.
            Bacteria; Bacillati; Actinomycetota; Actinomycetes;
            Propionibacteriales; Nocardioidaceae; Micropruina.
REFERENCE   1  (bases 1 to 5315)
  AUTHORS   Abdulkadir,N., Saraiva,J.P., Zhang,J., Stolte,S., Gillor,O.,
            Harms,H. and Rocha,U.
  TITLE     Genome-centric analyses of 165 metagenomes show that mobile genetic
            elements are crucial for the transmission of antimicrobial
            resistance genes to pathogens in activated sludge and wastewater
  JOURNAL   Microbiol Spectr 12 (3), e0291823 (2024)
   PUBMED   38289113
REFERENCE   2  (bases 1 to 5315)
  AUTHORS   Abdulkadir,N.
  TITLE     Direct Submission
  JOURNAL   Submitted (24-MAR-2023) Environmental Microbiology, Helmholtz
            Center for Environmental Research -UFZ, Permoserstr 15, Leipzig
            04318, Germany
COMMENT     The annotation was added by the NCBI Prokaryotic Genome Annotation
            Pipeline (PGAP). Information about PGAP can be found here:
            https://www.ncbi.nlm.nih.gov/genome/annotation_prok/
            
            ##Genome-Assembly-Data-START##
            Assembly Method        :: SPAdes v. 3.11.1
            Genome Representation  :: Full
            Expected Final Version :: Yes
            Genome Coverage        :: Not Applicable
            Sequencing Technology  :: Illumina MiSeq
            ##Genome-Assembly-Data-END##
            
            ##Genome-Annotation-Data-START##
            Annotation Provider               :: NCBI
            Annotation Date                   :: 05/04/2023 04:57:10
            Annotation Pipeline               :: NCBI Prokaryotic Genome
                                                 Annotation Pipeline (PGAP)
            Annotation Method                 :: Best-placed reference protein
                                                 set; GeneMarkS-2+
            Annotation Software revision      :: 6.5
            Features Annotated                :: Gene; CDS; rRNA; tRNA; ncRNA
            Genes (total)                     :: 2,776
            CDSs (total)                      :: 2,750
            Genes (coding)                    :: 2,714
            CDSs (with protein)               :: 2,714
            Genes (RNA)                       :: 26
            tRNAs                             :: 24
            ncRNAs                            :: 2
            Pseudo Genes (total)              :: 36
            CDSs (without protein)            :: 36
            Pseudo Genes (ambiguous residues) :: 0 of 36
            Pseudo Genes (frameshifted)       :: 2 of 36
            Pseudo Genes (incomplete)         :: 32 of 36
            Pseudo Genes (internal stop)      :: 2 of 36
            ##Genome-Annotation-Data-END##
FEATURES             Location/Qualifiers
     source          1..5315
                     /organism="Micropruina sp."
                     /mol_type="genomic DNA"
                     /submitter_seqid="NODE_6936_length_5315_cov_4.240114"
                     /isolate="AMR_MDS_2195"
                     /isolation_source="Activated sludge of municipal
                     wastewater treatment plants"
                     /db_xref="taxon:2737536"
                     /environmental_sample
                     /geo_loc_name="Canada"
                     /lat_lon="49.25 N 123.1 W"
                     /collection_date="2015-06-16"
                     /metagenome_source="wastewater metagenome"
                     /note="metagenomic"
     gene            complement(8..742)
                     /locus_tag="PLF56_06900"
     CDS             complement(8..742)
                     /locus_tag="PLF56_06900"
                     /EC_number="2.1.1.193"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_002527488.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_function: GO:0008168 - methyltransferase activity
                     [Evidence IEA];
                     GO_process: GO:0006364 - rRNA processing [Evidence IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="16S rRNA (uracil(1498)-N(3))-methyltransferase"
                     /protein_id="HOQ53327.1"
                     /translation="MTEAMFLADLDDPRVGDQVAVTGDEGRHAAIVRRIRKGEVVLVS
                     NGIGLAVRGPVLTSDVNGLVVEVAEVLVADEPSGLRFTVVQALAKGDRSELALEMLTE
                     LGVDEIVPWQAARSVVRWAPDRVERALVRWRSIVRESVKQSRRFRVPRVSPPLTTAEL
                     ALRIAEPALTLVLHEGATEWITTVPLPQQGEVMLIVGPEGGLTPDELATFAEAGARTV
                     LIADGVLRTSTAGTVALAQLQALARA"
     gene            complement(739..1914)
                     /gene="dnaJ"
                     /locus_tag="PLF56_06905"
     CDS             complement(739..1914)
                     /gene="dnaJ"
                     /locus_tag="PLF56_06905"
                     /EC_number="1.8.4.-"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_002513806.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_function: GO:0005515 - protein binding [Evidence IEA];
                     GO_process: GO:0006457 - protein folding [Evidence IEA];
                     GO_process: GO:0009408 - response to heat [Evidence IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="molecular chaperone DnaJ"
                     /protein_id="HOQ53328.1"
                     /translation="MTKDYYDVLGVDRDASADQIKKAYRKLAMQYHPDVADSPDAAEK
                     FKQIGEAYEVLHDSNKRAMYDRGVDPMGGGGFSGAGGFGFPGGGAAGFDFTNLVDAMF
                     GGAGARGPRSRVRRGQDALVRLSLTLAEAAFGITKPLRVDTAVVCGTCNGTGAKDGSE
                     PVTCSTCHGQGDITQIQRSFLGDIRTTQPCPNCRGYGSVIPHPCGECSGEGRVRASRT
                     INVKIPPGVKAGNRIHLESQGEVGPGGGPAGDLYVELSVEAHEMFTRNGDDLEVVVRL
                     PMTAAALGTEVVLPTLEAERDDLDRGLASVRLTVPSGTQSGTRIPINGRGVPRLRGSG
                     RGDLGVTLLVQTPSRLDDHQRDLLRQLAEARGENNHGGEMGKQHKGVFGWLKDAFTQ"
     gene            complement(1917..2933)
                     /gene="hrcA"
                     /locus_tag="PLF56_06910"
     CDS             complement(1917..2933)
                     /gene="hrcA"
                     /locus_tag="PLF56_06910"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_002519052.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_component: GO:0005737 - cytoplasm [Evidence IEA];
                     GO_process: GO:0009408 - response to heat [Evidence IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="heat-inducible transcriptional repressor HrcA"
                     /protein_id="HOQ53329.1"
                     /translation="MLDDRKLDILRAIVTDYVSSQEPVGSRALVDRHNLNVSPATVRN
                     DMAVLEEEGYITQPHTSAGRIPTDKGYRLFVDRLGTVKPLSIAERRAIQTFMAGAIDV
                     DDVVRRTVRLLAQITQQVAIVQYPVLSRATVRLVELLSLAPGRGLVILVNSAGRVDQR
                     QLDFGALTDDHLASLRERVNVALVGQPINRVPDLAAALLDELAPELRPPASVVLAALI
                     ELVSADPATRVVVGGVPNLTRFGDQFETTVKPVLEALEEQVVLLRLLGESGGSADVTV
                     RIGSENPYEPLQSTSVVASAYSSGDDVWASMGIVGPTRMDYPGTMASVRAVARYVGRI
                     LAEG"
     gene            3149..3532
                     /locus_tag="PLF56_06915"
     CDS             3149..3532
                     /locus_tag="PLF56_06915"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_008359647.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="DUF1304 domain-containing protein"
                     /protein_id="HOQ53330.1"
                     /translation="MTVLILIIAALAGLLHVFIFTLESFRWEEAGTRRSFGTTAEQAA
                     MTRQLAYNQGFYNLFLAITAFAGIALAFWPPVSTALLVVSLGSMLAAAVVLVSSDRTK
                     LRAAAIQGTLPLIGLVLLAVRALQG"
     gene            3667..4518
                     /locus_tag="PLF56_06920"
     CDS             3667..4518
                     /locus_tag="PLF56_06920"
                     /inference="COORDINATES: protein motif:HMM:NF012955.2"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_function: GO:0016787 - hydrolase activity [Evidence
                     IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="MBL fold metallo-hydrolase"
                     /protein_id="HOQ53331.1"
                     /translation="MDFSLTPFETLGPGAWRAVAQPAAVNLGLVAGSSGALLIDTGSS
                     PAQGAEIRAAAEAVLEVPLVGVVVTHAHFDHYYGLAAFDDLPTYGHESLIDALTAEQV
                     GDEAPRLGFAVNELRPPNRPLALARMIDLGDRHAEIVHFGRGHTAGDLVVIVPDLDLI
                     FTGDLLEQSAPPAMGPDCFLKEWPSALDGILGLVNERTRLMPGHGEPFDRTFAFTQRA
                     EISSVYGQVEYLIGQGIKQEDALAAGEWQFDDATIEAVLPIAYAQLAAEGKVPRPTLR
                     LLTQRNP"
     gene            complement(4625..>5315)
                     /locus_tag="PLF56_06925"
     CDS             complement(4625..>5315)
                     /locus_tag="PLF56_06925"
                     /inference="COORDINATES: protein motif:HMM:NF012673.2"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=2
                     /transl_table=11
                     /product="inositol monophosphatase"
                     /protein_id="HOQ53332.1"
                     /translation="EKGPGDVVTVADRLAEQALTAIFRQHDPGCLVVGEEGVYADPAP
                     LRALPTAEHAWVIDPVDGTRNFTNGSPDFAVMVAEVRAGVTTRSWIWQPVHERMYYAE
                     LGGGVYRNGERLEVAAPSEPLVGALHGRYGRPREGLGDEVQLVRLAGCCGVDYPHVIE
                     GRRAFLGYWSMHPWDHLPGGLMVTEQGGVLATIEGEQYRAGVSGRYLLAAATPALWQR
                     AADVVQQQFRH"
BASE COUNT          819 a         1921 c         1759 g          816 t
ORIGIN      
        1 gaggcggtca ggcacgcgcc agcgcctgca actgcgcaag tgccacggtg ccggccgtgg
       61 acgtgcgcag cacgccgtct gcgatcagca cggtgcgcgc gccggcctcg gcgaaggtgg
      121 ccagctcatc gggggtgagt cccccttcgg gaccgacgat gagcatcacc tcgccctgct
      181 gcggcagcgg caccgtcgtg atccactcgg tcgcaccctc gtgcagcacc agggtcaacg
      241 ccggctcggc gatgcgcaac gccagctcgg cggtggtcag cggcggactg acccgcggca
      301 cccggaagcg gcgcgactgc ttgaccgact cgcggacgat actgcgccag cggaccagtg
      361 cacgctccac ccggtcgggc gcccacctga ccaccgagcg cgcggcctgc cagggcacga
      421 tctcgtccac acccagctcg gtcagcatct cgagcgccag ttcggaacgg tcgcccttgg
      481 ccagcgcctg caccacggtg aaccgcagcc cggacggctc gtcggcgacc agcacctcgg
      541 ccacctcgac caccaggccg ttcacgtcag aggtcagcac gggaccacgc acggccagcc
      601 cgatgccgtt gctgacgagc accacctcgc ccttgcgaat gcgcctgacg atggctgcgt
      661 ggcgaccctc atcgccggtc accgccacct gatccccgac ccgggggtcg tccagatctg
      721 ccaggaacat tgcctcggtc attgtgtgaa cgcgtccttc agccagccga aaacaccctt
      781 gtgctgcttg cccatctcgc cgccgtggtt gttctcgccg cgggcctcgg ccagttgccg
      841 caggaggtcg cgctggtgat cgtccaaacg gctcggtgtc tgcaccagca gcgtaacgcc
      901 cagatcgccc cgtccggagc cccgaagccg cggcaccccg cgtccgttga tcggaatgcg
      961 cgtgcccgac tgggtgccgc tgggcacggt cagccggacg gacgccagcc cgcggtccag
     1021 atcgtcgcgt tcggcctcca gcgtcggcag cacgacctcg gtgcccagcg ccgccgccgt
     1081 catcggcaac cggaccacga cttctaggtc gtcgccgttg cgggtgaaca tctcatgcgc
     1141 ctcgacgctc agttcgacgt acaggtcacc ggccggaccc ccgccgggcc cgacctcgcc
     1201 ctgcgactcg agatggatgc ggttgccggc cttgaccccc ggcggaatct tcacgttgat
     1261 cgtgcgggac gcccgcaccc ggccctcgcc cgagcactca ccgcagggat gcgggatcac
     1321 cgagccgtag ccgcggcagt tggggcacgg ctgggtggtg cggatgtcgc cgaggaacga
     1381 ccgctgaatc tgcgtgatgt cgccctgccc gtggcaggtg gaacaggtga ccggctcgga
     1441 gccgtccttg gcgccggtgc cgttgcaggt gccgcagacc accgcggtgt cgacccgcag
     1501 cggcttggtg atgccgaacg ccgcctcggc cagggtcagc gagagccgca ccagcgcgtc
     1561 ctgaccgcgc cgcacccgcg agcgcgggcc gcgcgcgccc gcgccaccga acatggcgtc
     1621 caccaggttg gtgaagtcga agccggccgc acccccaccg ggaaaaccga accccccggc
     1681 gccgctgaag ccaccaccgc ccatcggatc gacgccgcgg tcgtacatgg cccgcttgtt
     1741 gctgtcgtgc agcacctcgt acgcctcgcc gatctgcttg aacttctcgg cggcatcggg
     1801 gctgtcggcc acgtcggggt ggtactgcat ggccagcttg cggtaggcct tcttgatctg
     1861 gtcggcgctg gcgtcgcggt caacgcccag cacgtcgtag tagtccttgg tcaacgtcat
     1921 ccttctgcca agatgcggcc aacgtaacgg gcgactgccc gcaccgaagc catggtgccc
     1981 gggtaatcca tgcgggtggg tccgacgatg cccatgctcg cccacacgtc atcgccggaa
     2041 ctgtacgcac tggcgacgac cgaggtcgac tgcaacggct cgtaggggtt ttcgctgccg
     2101 atccgaacgg tgacgtcggc ggaccctcca gactcaccca acaaccggag tagtacgact
     2161 tgttcctcca acgcttcgag caccggtttc acggtggtct cgaactgatc gccgaagcgg
     2221 gtcaggttgg gcacgccgcc gaccaccacg cgggtggccg ggtcggccga aacgagttcg
     2281 atcaacgccg ccagtaccac cgaggcgggc ggacgcagct cgggcgcgag ttcgtccagc
     2341 agggcggcag ccaggtcggg gacgcggttg atgggctgcc cgaccagcgc gacgttgacc
     2401 cgttcgcgca gcgaggccag gtggtcgtcg gtgagcgcgc cgaagtcgag ttggcgctgg
     2461 tcgacgcgtc cggccgagtt gaccaggatc accaggccgc ggccgggcgc caggctcagc
     2521 aactcgacca gccggacggt ggcccgcgac agcaccgggt actgcacgat cgccacctgc
     2581 tgggtgatct gcgccagcaa ccggacggtg cgccgcacca cgtcgtcgac gtcgatggcg
     2641 ccggccatga aggtctggat cgcccggcgc tcggcgatcg acagcggttt cacggtgccc
     2701 aaccggtcga cgaagaggcg atagcccttg tcagtgggga ttcggccggc gctggtgtgg
     2761 ggctgggtga tgtagccctc ttcttcgagc accgccatgt cgttgcgcac ggtggccggg
     2821 gagacgttca ggttgtgccg atcgaccagg gcgcgcgagc ccaccggctc ctgcgagctc
     2881 acgtagtcgg tgacgatggc ccgcagaatg tcgagcttgc gatcgtccaa cacgggggcc
     2941 tccttcacgc gtctggcact ctcgatgatc gagtgccagt ctagacgaag cagccgccca
     3001 ggtaggtgag cgtggccggc gcaacccgat cgcacgcccg gctcggtgcc gcgttggaca
     3061 ccccccaagg cacgagaggc gccgcctcgg acaaacggca catgccgcac actacgctgg
     3121 cgcctatctg cgaacctgcg aggttgccgt gaccgtactc atcctgatca tcgccgcact
     3181 ggccgggttg ctgcacgtgt tcatcttcac cctggaatcg ttcaggtggg aggaggccgg
     3241 cacgcgccgc tccttcggca ccacggccga acaggccgcg atgacccggc aattggccta
     3301 caaccagggg ttctacaacc tttttctagc catcaccgcc ttcgccggca tcgcattggc
     3361 cttctggccg ccggtgtcga ccgcgctgct ggtggtgagc ctcggctcga tgctcgcggc
     3421 cgcggtggtg ctcgtttcat ccgaccgcac gaagctgcgg gccgccgcga tccagggcac
     3481 cctgccgctg atcgggttgg tactgctggc ggtacgcgcg ctgcagggat gaatgtgccg
     3541 gccggctgaa cagcgggtgg gttttggcac agcagtcggt gccaaaaccc acccgttgat
     3601 gcagagcccc tgaatctcga tctggagcgg ctgaacgggt actgagccga cgcataggct
     3661 ggcccggtgg acttctccct gacgccgttc gagacactcg ggcccggcgc ctggcgtgcc
     3721 gtcgcccagc ccgccgcggt caacctcggc ctggtcgccg ggtcgtccgg tgccctcctg
     3781 atcgacaccg gctcgtcacc ggcccaggga gccgagatca gggctgcggc cgaggccgtg
     3841 ctcgaggttc cgctggtcgg cgtggtggtc acgcacgcgc acttcgacca ctactacggg
     3901 ctggccgcct tcgatgacct gcccacctac gggcacgaga gcctgatcga cgcactgacg
     3961 gccgagcagg tcggcgacga ggcccccagg ctgggcttcg ctgtgaacga gttgcggccg
     4021 ccgaaccgtc cactggcgct ggcccgcatg atcgacctgg gcgaccggca cgccgagatc
     4081 gtccacttcg gacggggtca caccgccggc gacctggtcg tgatcgtgcc cgacctggac
     4141 ctgatcttca ccggcgacct gctggagcag tccgctcccc cggcgatggg accggactgc
     4201 tttctcaagg aatggccctc ggcgctggac ggcattctcg ggctggtcaa cgaacgcacc
     4261 cggctgatgc cgggccacgg cgagccgttc gaccgcacat tcgccttcac ccagcgggcc
     4321 gagatctcgt cggtgtacgg ccaggtcgag tacctgatcg gtcagggcat caagcaggag
     4381 gacgcattgg ccgcaggcga atggcagttc gacgacgcca ccatcgaggc cgtgctgccg
     4441 atcgcctatg cgcaactggc ggccgagggc aaggtgcccc ggccgacact gcggctgctg
     4501 acccagcgca acccctgatc accccaccga tggtggagcc ggtcgatacc cccgccaccc
     4561 actcacgagg tctcgacacg ttcggccaac gatcggcgat ccttggtcgg cccggcggtg
     4621 ggtttcagtg acgaaactgc tgctgcacca cgtctgcggc gcgctgccac agggccggcg
     4681 tcgcggccgc gagcaggtag cgtccgctca ccccggcgcg gtactgctcg ccctcgatgg
     4741 tcgccagcac gcccccctgt tcggtgacca tcaggccacc cggcaggtgg tcccacgggt
     4801 gcatcgacca gtaaccgaga aaggcccgcc gcccctcgat cacatgcgga tagtcgacgc
     4861 cacaacagcc ggccagccgc accagctgca cctcgtcgcc cagaccctcg cgcgggcgtc
     4921 cgtaccggcc gtgcagcgcc cccaccaacg gctcggacgg cgccgccacc tcgagccgct
     4981 cgccgttgcg gtacacaccg ccgccgagtt cggcgtagta catgcgctca tgcacgggct
     5041 gccagatcca gctgcgggtg gtcaccccgg cgcgcacctc tgccaccatc accgcgaaat
     5101 cagggctgcc gttggtgaag ttgcgggtgc cgtccaccgg atctatcacc caggcatgct
     5161 cggccgtggg tagggcacgc agcggggccg ggtcggcgta gacaccctct tcgccgacca
     5221 ccaggcaccc ggggtcgtgc tgccgaaaga tcgcggtcag cgcctgttcg gccagccggt
     5281 cggcgacggt caccacgtcg cccggaccct tctcg
//
LOCUS       DAOSUG010000174         5310 bp    DNA     linear   ENV 10-JUN-2024
DEFINITION  MAG TPA_asm: Micropruina sp. isolate AMR_MDS_2195
            NODE_6944_length_5310_cov_4.085252, whole genome shotgun sequence.
ACCESSION   DAOSUG010000174 DAOSUG010000000
VERSION     DAOSUG010000174.1
DBLINK      BioProject: PRJNA925477
            BioSample: SAMN32864262
            Sequence Read Archive: SRR4236673
KEYWORDS    WGS; ENV; Metagenome Assembled Genome; MAG; Third Party Data; TPA;
            TPA:assembly.
SOURCE      Micropruina sp. (wastewater metagenome)
  ORGANISM  Micropruina sp.
            Bacteria; Bacillati; Actinomycetota; Actinomycetes;
            Propionibacteriales; Nocardioidaceae; Micropruina.
REFERENCE   1  (bases 1 to 5310)
  AUTHORS   Abdulkadir,N., Saraiva,J.P., Zhang,J., Stolte,S., Gillor,O.,
            Harms,H. and Rocha,U.
  TITLE     Genome-centric analyses of 165 metagenomes show that mobile genetic
            elements are crucial for the transmission of antimicrobial
            resistance genes to pathogens in activated sludge and wastewater
  JOURNAL   Microbiol Spectr 12 (3), e0291823 (2024)
   PUBMED   38289113
REFERENCE   2  (bases 1 to 5310)
  AUTHORS   Abdulkadir,N.
  TITLE     Direct Submission
  JOURNAL   Submitted (24-MAR-2023) Environmental Microbiology, Helmholtz
            Center for Environmental Research -UFZ, Permoserstr 15, Leipzig
            04318, Germany
COMMENT     The annotation was added by the NCBI Prokaryotic Genome Annotation
            Pipeline (PGAP). Information about PGAP can be found here:
            https://www.ncbi.nlm.nih.gov/genome/annotation_prok/
            
            ##Genome-Assembly-Data-START##
            Assembly Method        :: SPAdes v. 3.11.1
            Genome Representation  :: Full
            Expected Final Version :: Yes
            Genome Coverage        :: Not Applicable
            Sequencing Technology  :: Illumina MiSeq
            ##Genome-Assembly-Data-END##
            
            ##Genome-Annotation-Data-START##
            Annotation Provider               :: NCBI
            Annotation Date                   :: 05/04/2023 04:57:10
            Annotation Pipeline               :: NCBI Prokaryotic Genome
                                                 Annotation Pipeline (PGAP)
            Annotation Method                 :: Best-placed reference protein
                                                 set; GeneMarkS-2+
            Annotation Software revision      :: 6.5
            Features Annotated                :: Gene; CDS; rRNA; tRNA; ncRNA
            Genes (total)                     :: 2,776
            CDSs (total)                      :: 2,750
            Genes (coding)                    :: 2,714
            CDSs (with protein)               :: 2,714
            Genes (RNA)                       :: 26
            tRNAs                             :: 24
            ncRNAs                            :: 2
            Pseudo Genes (total)              :: 36
            CDSs (without protein)            :: 36
            Pseudo Genes (ambiguous residues) :: 0 of 36
            Pseudo Genes (frameshifted)       :: 2 of 36
            Pseudo Genes (incomplete)         :: 32 of 36
            Pseudo Genes (internal stop)      :: 2 of 36
            ##Genome-Annotation-Data-END##
FEATURES             Location/Qualifiers
     source          1..5310
                     /organism="Micropruina sp."
                     /mol_type="genomic DNA"
                     /submitter_seqid="NODE_6944_length_5310_cov_4.085252"
                     /isolate="AMR_MDS_2195"
                     /isolation_source="Activated sludge of municipal
                     wastewater treatment plants"
                     /db_xref="taxon:2737536"
                     /environmental_sample
                     /geo_loc_name="Canada"
                     /lat_lon="49.25 N 123.1 W"
                     /collection_date="2015-06-16"
                     /metagenome_source="wastewater metagenome"
                     /note="metagenomic"
     gene            complement(<1..1337)
                     /locus_tag="PLF56_06930"
     CDS             complement(<1..1337)
                     /locus_tag="PLF56_06930"
                     /EC_number="2.4.-.-"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_014845746.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="glycosyltransferase"
                     /protein_id="HOQ53333.1"
                     /translation="MSTPIITNDFAPVDGLVPESRRARTKNPYTPVMIAVSIAATLGI
                     LAYAWFLLNPNNRGDLLPWAMVMIAEVILIFHALMAMWAILAGQKDPRTFAFHDAQRA
                     LYDPAANERQGVTDDPTQWPLHLDGEPVTADLLITVYGEPLDVIRRTAEAAKAVRGAH
                     QTWILDDGKSDAVRDMAAAMQIGYIRRLTNHGAKAGNVNNALTIAKGDYFVILDADFV
                     PRPEFLEETLPFMIDSNVAFVQTPQTYGNMHNIISRGAGYMQTMFYKFIQPGRTQFNA
                     AFCVGTNVLFRRSAVLDIGGMYTQSKSEDVWTSLMLHERGWKSIFIPKTLAVGDAPET
                     IEAYTKQQQRWATGGFEILFTHNPFSPKRKLTLDQRFMYFVTASFYLTGIAPGILLFV
                     PALEVFFDLHPVNLQVGVWTWLLFYAGFYVLQILLAALTLGTFRWEVLLLAANS"
     gene            complement(1540..3216)
                     /locus_tag="PLF56_06935"
     CDS             complement(1540..3216)
                     /locus_tag="PLF56_06935"
                     /inference="COORDINATES: protein motif:HMM:NF021489.2"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_component: GO:0005886 - plasma membrane [Evidence IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="DUF2207 domain-containing protein"
                     /protein_id="HOQ53334.1"
                     /translation="MLLVCCAPGAWAASDDQIDTFDVTITVNADGSLLVRERLVWRFG
                     VNSTRHGIERWLVTRRPFDDQRDEVYDISNPTVTSSSQPSPVVSVQRDLGSGRTRLMR
                     IRVGDSDRTLPGPTAGYELSYTVKGAVRDFADFDEVGWNVTGPRLPKVISASGTLRAP
                     GGVRKVSCSTGSAGTGGRCTSGPVDGGVGSFAATSLAAGSPMTVVATIGDGLVQGAGP
                     TLVESSDLVDQRTNRFALVAGLVGALLVPLLGWWYYRRHGHDLRFDEVPAGELPEPGA
                     SVTQVRDHRVEPPVRKSPPPLALAEAGLLLAGRSSTRQTTATLVGLAVDGAIRLRVGA
                     DPEARLLESRRARDRSSAVLLEELFDGGGTVADLRAPGVLAEAHERILADARSRAEHE
                     QWFVRPPRTRSVGVLVIAALALGYIAYLVIGVAVLYLTPLWASTVITLVVLNRGLQRG
                     QRTALGRALTDQVQGFRTHLATVTAEQLSDEADDDLYSRYLPWAIMFDLTHRWTRVCA
                     QLVAQGRITGAAPTWYVGSQWSLRGFAGPVDSLSAQVRAATTATDLAGSA"
     gene            complement(3267..3740)
                     /gene="smpB"
                     /locus_tag="PLF56_06940"
     CDS             complement(3267..3740)
                     /gene="smpB"
                     /locus_tag="PLF56_06940"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_016670251.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_function: GO:0003723 - RNA binding [Evidence IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="SsrA-binding protein SmpB"
                     /protein_id="HOQ53335.1"
                     /translation="MPREKGRILVAQNRRARHDYHLGERFEAGLVLTGTEVKSLRLGR
                     ASLADAFATVDDGEVWLRNAHIPEYAFGTWTNHSARRNRKLLLHRREIERLDRATTAS
                     GRTIVPLALYFLDGYAKVELAVGTGKKDWDKRQSIAEREANRDAQRALAARNRGE"
     gene            complement(3810..5138)
                     /locus_tag="PLF56_06945"
     CDS             complement(3810..5138)
                     /locus_tag="PLF56_06945"
                     /inference="COORDINATES: protein motif:HMM:NF013700.2"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="peptidoglycan DD-metalloendopeptidase family
                     protein"
                     /protein_id="HOQ53336.1"
                     /translation="MTSPTCTKDLRGTTLRLLRPLIAAVAVSLAVSLGSPARADDLKD
                     RRDDVRKAIVASKQNVSESKAEMSKAVAALAKARSALAKAQRNLSAATDAVSEARSVD
                     EAIGRRLSAARTKLAAAKQAVSKAKAAVDAQLTLMGQAAREAYQQQSDLVGLSVVFDS
                     ADTGDLSQRLQWSTTIFDATSAQMTRLRALQATLVAAQKAQAEIEKQIAADKAEAAAN
                     LATLKVLQSKAALAKNAVATKVLDLGKAQKAARSDLDADQAAYSKLQAQEAKISAEIK
                     RRAEIARKKALAAEAKRRAAAKKAGKTYVPRSVSSKGFIRPVNASPGSPFGLRFHPIL
                     HVWRMHRGTDFGAACGLPIYAANDGRVASAGRQGGFGNYTVIDHGLIGGKYISTGYAH
                     QSRIIVHRGQRVKQGQLIGYVGTTGLSTGCHLHLQVYLNGGVVNPLTYIP"
BASE COUNT          852 a         1786 c         1809 g          863 t
ORIGIN      
        1 gagttcgccg ccagcagcag cacctcccag cggaaggtac ccagggtgag cgcggccagc
       61 aggatctgca gcacgtagaa gccggcgtag aacagcagcc aggtccacac gcccacctgc
      121 aggttcaccg ggtgcaggtc gaagaacacc tcgagggccg gaacgaacag cagaatgccg
      181 ggggcgatgc cggtcaggta gaacgacgcg gtgacgaaat acatgaaccg ctggtccagc
      241 gtcagcttgc gcttggggct gaacgggttg tgcgtgaaca gaatctcgaa cccgccggtg
      301 gcccagcgct gttgctgctt ggtgtaggcc tcgatggtct cgggggcgtc accgacggcc
      361 agcgtcttgg gaatgaagat cgacttccag ccccgctcgt gcagcatcag cgaggtccag
      421 acgtcctcgg acttcgactg ggtgtacatg ccgccgatgt cgagcacggc gctgcgccgg
      481 aacaacacgt tggtgcccac gcagaaggcc gcgttgaact gggtgcggcc gggctggatg
      541 aacttgtaga acatcgtctg catgtagccc gcgccgcggc tgatgatgtt gtgcatgttg
      601 ccgtaggtct gcggcgtctg tacgaaggcg acgttggagt cgatcatgaa cggcagggtc
      661 tcttcgagaa actccggacg gggcacgaag tcggcgtcca ggatcacgaa gtagtcaccc
      721 ttggcgatgg tgagcgcgtt gttgacgttg cccgccttgg cgccatggtt ggtgagccgg
      781 cggatgtagc cgatctgcat cgcagctgcc atgtcgcgta cggcgtcgga cttgccgtcg
      841 tccaggatcc aggtctgatg ggccccgcgc acggccttgg cggcctcggc ggtacgccgg
      901 atcacgtcca gcggctcgcc gtacacggtg atcagcaggt cggccgtcac gggctccccg
      961 tccaggtgca acggccactg ggtggggtcg tcggtcacgc cctgccgctc gttggcggcc
     1021 gggtcgtaca gggcccgctg cgcgtcgtgg aaggcgaagg tgcgggggtc cttctgaccg
     1081 gccaggatcg cccacatcgc catcagggcg tgaaagatca agatcacctc ggcgatcatc
     1141 accatcgccc agggcagcag gtctccccgg ttgttcggat tcaacagaaa ccaggcgtag
     1201 gccaggatgc ccagggtcgc cgcgatggag acggcgatca tgacgggggt gtaggggttc
     1261 ttggttctcg cccgccggga ttcaggcacg aggccatcga ccggggcgaa gtcgttggtg
     1321 atgatgggtg tagacataac tctcccttgg gagcgcatct tcggatttgc tgtgagcctc
     1381 tgccgcggtc tgccgcgggg gcttggttca ccggccggtg aagccggctt cggggagttc
     1441 gggagtcgtt cctcgccacg aagttgatac caggataagc aggtcccgtc ggaaaagcaa
     1501 aatattggtc tcagatacta atatttgtcc gaacgtgcgt caggctgagc cggccaggtc
     1561 tgtcgcggtg gtcgccgcac gcacctgggc gctcaacgaa tcgaccggtc cggcgaaccc
     1621 tcgcagactc cactgcgacc cgacgtacca ggtcggcgcc gcgccggtga tccggccctg
     1681 ggccaccagt tgcgcgcaga cccgggtcca tcggtgggtc aggtcgaaca tgatcgccca
     1741 cggcagatag cgggaataga ggtcgtcgtc ggcctcgtcg ctcaactgct cggcggtgac
     1801 cgtcgcgaga tgcgtgcgga aaccctgcac ctgatcggtc agcgcacgac ccagggcggt
     1861 gcgctgcccc cgttgaagtc cacgattcag cacgaccagc gtgatcaccg tcgaggccca
     1921 cagcggcgtg agatacagca ctgccacacc gatcacgaga tacgcgatgt agcccagcgc
     1981 cagggccgcg atcaccagca cgccgaccga acgggtccgg ggcggtcgta cgaaccactg
     2041 ctcatgctcg gcccgcgacc gggcgtcagc gagaatgcgc tcatgggcct cggcgagcac
     2101 cccgggggcc ctcaggtcgg cgacggtgcc cccgccgtcg aacaattcct ccaacagaac
     2161 cgcggacgag cggtcgcggg cgcggcgcga ttcgagcagc cgcgcttcgg ggtccgcacc
     2221 gacgcgcagc cggatcgccc cgtccaccgc cagcccgacc agggtggcgg tggtctgccg
     2281 tgtcgaggag cgtccggcca gcaagaggcc cgcctccgcc agggcgagcg gtggcggaga
     2341 ctttcggacc ggcggctcca cccgatgatc gcgcacctgc gtgacggacg cgccgggctc
     2401 gggcagctcg cccgccggca cctcgtcgaa ccggaggtcg tggccgtggc ggcggtagta
     2461 ccaccagccc agcagtggca ccaacaatgc ccccaccagc ccggcgacca gcgcgaaccg
     2521 gttggttcgc tgatcgacca ggtcggaact ctcgaccagc gtcgggccgg cgccctggac
     2581 gagtccgtcg ccgatcgtgg cgaccactgt catcgggctg cctgcggcca ggctcgtggc
     2641 ggcgaaactg ccgactccgc catcgacggg tccggacgtg caccggccac cggttcccgc
     2701 tgacccggtc gagcaggaga ctttgcggac acccccgggt gcacgcaacg tcccggatgc
     2761 ggagatcact ttcggaagcc gcggccccgt cacgttccag cccacctcat cgaagtcggc
     2821 gaaatctcgc acagcgccct tcacggtgta gctgagttcg tagccggccg tcggcccggg
     2881 cagcgtgcga tcggagtcac cgacccggat gcgcatcaag cgcgtgcgtc cagaacccag
     2941 atcgcgctgc accgagacca ccggcgacgg ctggctcgag ctggtcaccg tagggttgct
     3001 gatgtcgtag acctcgtcgc gctgatcgtc gaacggtcgc cgggtgacca gccagcgctc
     3061 gattccgtgc ctggtcgagt tcaccccgaa acgccacacc aggcgctccc ggaccagcaa
     3121 cgacccgtcg gcgttgacgg tgatggtgac gtcgaacgtg tcgatctggt cgtcgctcgc
     3181 ggcccatgcg ccgggggcgc agcagacaag cagcacaatg gtcagcgcaa cccagcccgg
     3241 ccaacgccgc ggccggccgc cacgcatcat tcgccccggt ttcgcgccgc caatgcccgc
     3301 tgggcgtcgc gattggcctc acgctcggcg atggactggc gcttgtccca gtccttcttg
     3361 ccggtgccga cggcgagttc aaccttggcg tagccgtcga gaaagtagag cgccaacggc
     3421 acgatcgtgc gaccggacgc agtggtggcc cgatcgagcc gctcgatctc acgccggtgc
     3481 agcagcagct tgcggttgcg gcgggcggag tggttggtcc aggtgccgaa ggcgtactcg
     3541 ggaatgtgtg cgttgcgcag ccacacctcg ccgtcatcga cggtcgcgaa cgcgtcggcc
     3601 agcgacgcgc ggcccaggcg cagcgacttg acctcggtgc ccgtcagcac caggcccgcc
     3661 tcgaaccgct cgcccaggtg gtagtcatga cgcgcccgcc gattctgggc gaccagaatg
     3721 cgccccttct cacgtggcat cggcgtcgcg ctcccccggt ggtggacaga tcggtgttca
     3781 ccttaccccg gcagcgcgac gccgcggatt cacggaatgt acgtcagcgg gttcaccaca
     3841 ccgccgttga ggtacacctg aaggtgcagg tggcagccgg tggacaggcc ggtggtgccc
     3901 acgtagccga tcaactgacc ctgcttcacc cgctggccgc ggtggacgat gatccgcgac
     3961 tggtgcgcgt agccggtcga gatgtacttg ccgccgatca gaccgtgatc gatcacggtg
     4021 tagttgccga agccgccctg ccggccggca ctggccaccc gtccgtcgtt ggccgcatag
     4081 atcggcagtc cgcaggccgc tccgaagtcg gtgccgcggt gcatccgcca cacgtgcaag
     4141 atggggtgga accgcaggcc gaacggcgac ccgggtgagg cgttgaccgg ccggatgaag
     4201 cccttggacg acaccgaccg cggcacgtag gtcttgccgg ccttcttggc ggcggcccgg
     4261 cgcttggcct cggccgccag cgccttcttg cgggcgatct ctgcgcgccg cttgatctcg
     4321 gcggaaatct tcgcctcctg cgcctgcagc ttggaatagg ccgcctggtc ggcgtccagg
     4381 tcggaccggg ccgccttctg cgccttgccg agatcgagca ccttggtggc cacggcgttc
     4441 ttggccagcg cggccttgct ctgcagcacc ttcagcgtcg ccaggttggc ggccgcctcg
     4501 gccttgtcgg ccgcgatctg cttctcgatc tcggcctgcg ccttctgggc cgccacgagg
     4561 gtggcctgca gggcacgcag ccgcgtcatc tgggcgctgg tggcgtcgaa gatcgtggtg
     4621 gaccactgca gccgctgcga aaggtcgccg gtgtcggccg agtcgaacac caccgacagc
     4681 cccaccaggt cgctctgctg ctggtaggcc tcgcgggccg cctggcccat caaggtgagt
     4741 tgcgcatcga cggcggcctt ggccttgctg acggcctgct tggcggcggc cagcttcgtg
     4801 cgggccgcgc tcagccggcg tccgatcgcc tcgtcgaccg accgagcctc gctcaccgca
     4861 tcggtggccg cgctcaggtt gcgctgcgcc ttcgccaacg ctgagcgggc cttggccagg
     4921 gcggcgaccg ccttcgacat ctcggccttg gattccgaaa cgttctgctt cgacgcgacg
     4981 atcgccttgc ggacgtcgtc gcggcgatcc ttcaggtcgt cggcgcgggc cggcgagccg
     5041 agactcaccg cgagcgagac ggcgaccgcg gcgatcagcg gacgcagcag gcgcagcgtc
     5101 gtgccgcgca gatccttcgt gcaggtgggg gaagtcacct ggttcacggt ccggccccaa
     5161 tcagtcggtt gaggatccgg tgtgggctag accctcaagt actttcgtgt ggcgatcagc
     5221 gtgggaacca cagaaagcac gatgcccacg gcgatgaccc cgagcattgc caacccgacg
     5281 tgtgaccagc caatccactc cacactcttg
//
LOCUS       DAOSUG010000175         5309 bp    DNA     linear   ENV 10-JUN-2024
DEFINITION  MAG TPA_asm: Micropruina sp. isolate AMR_MDS_2195
            NODE_6947_length_5309_cov_4.532547, whole genome shotgun sequence.
ACCESSION   DAOSUG010000175 DAOSUG010000000
VERSION     DAOSUG010000175.1
DBLINK      BioProject: PRJNA925477
            BioSample: SAMN32864262
            Sequence Read Archive: SRR4236673
KEYWORDS    WGS; ENV; Metagenome Assembled Genome; MAG; Third Party Data; TPA;
            TPA:assembly.
SOURCE      Micropruina sp. (wastewater metagenome)
  ORGANISM  Micropruina sp.
            Bacteria; Bacillati; Actinomycetota; Actinomycetes;
            Propionibacteriales; Nocardioidaceae; Micropruina.
REFERENCE   1  (bases 1 to 5309)
  AUTHORS   Abdulkadir,N., Saraiva,J.P., Zhang,J., Stolte,S., Gillor,O.,
            Harms,H. and Rocha,U.
  TITLE     Genome-centric analyses of 165 metagenomes show that mobile genetic
            elements are crucial for the transmission of antimicrobial
            resistance genes to pathogens in activated sludge and wastewater
  JOURNAL   Microbiol Spectr 12 (3), e0291823 (2024)
   PUBMED   38289113
REFERENCE   2  (bases 1 to 5309)
  AUTHORS   Abdulkadir,N.
  TITLE     Direct Submission
  JOURNAL   Submitted (24-MAR-2023) Environmental Microbiology, Helmholtz
            Center for Environmental Research -UFZ, Permoserstr 15, Leipzig
            04318, Germany
COMMENT     The annotation was added by the NCBI Prokaryotic Genome Annotation
            Pipeline (PGAP). Information about PGAP can be found here:
            https://www.ncbi.nlm.nih.gov/genome/annotation_prok/
            
            ##Genome-Assembly-Data-START##
            Assembly Method        :: SPAdes v. 3.11.1
            Genome Representation  :: Full
            Expected Final Version :: Yes
            Genome Coverage        :: Not Applicable
            Sequencing Technology  :: Illumina MiSeq
            ##Genome-Assembly-Data-END##
            
            ##Genome-Annotation-Data-START##
            Annotation Provider               :: NCBI
            Annotation Date                   :: 05/04/2023 04:57:10
            Annotation Pipeline               :: NCBI Prokaryotic Genome
                                                 Annotation Pipeline (PGAP)
            Annotation Method                 :: Best-placed reference protein
                                                 set; GeneMarkS-2+
            Annotation Software revision      :: 6.5
            Features Annotated                :: Gene; CDS; rRNA; tRNA; ncRNA
            Genes (total)                     :: 2,776
            CDSs (total)                      :: 2,750
            Genes (coding)                    :: 2,714
            CDSs (with protein)               :: 2,714
            Genes (RNA)                       :: 26
            tRNAs                             :: 24
            ncRNAs                            :: 2
            Pseudo Genes (total)              :: 36
            CDSs (without protein)            :: 36
            Pseudo Genes (ambiguous residues) :: 0 of 36
            Pseudo Genes (frameshifted)       :: 2 of 36
            Pseudo Genes (incomplete)         :: 32 of 36
            Pseudo Genes (internal stop)      :: 2 of 36
            ##Genome-Annotation-Data-END##
FEATURES             Location/Qualifiers
     source          1..5309
                     /organism="Micropruina sp."
                     /mol_type="genomic DNA"
                     /submitter_seqid="NODE_6947_length_5309_cov_4.532547"
                     /isolate="AMR_MDS_2195"
                     /isolation_source="Activated sludge of municipal
                     wastewater treatment plants"
                     /db_xref="taxon:2737536"
                     /environmental_sample
                     /geo_loc_name="Canada"
                     /lat_lon="49.25 N 123.1 W"
                     /collection_date="2015-06-16"
                     /metagenome_source="wastewater metagenome"
                     /note="metagenomic"
     gene            complement(<1..374)
                     /locus_tag="PLF56_06950"
     CDS             complement(<1..374)
                     /locus_tag="PLF56_06950"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_011755383.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="alcohol dehydrogenase catalytic
                     domain-containing protein"
                     /protein_id="HOQ53337.1"
                     /translation="MKALTWQGVEDVRVDSVPDPSIEQPTDAVVRVTSTAICGSDLHL
                     YGVLAPFLTKGDVLGHEFMGIVEQVGDEVGNLHVGDRVVVPFNISCGHCWMCSRGLFA
                     QCETTQNTAQGKGASLFGFSSL"
     gene            complement(371..2587)
                     /locus_tag="PLF56_06955"
     CDS             complement(371..2587)
                     /locus_tag="PLF56_06955"
                     /EC_number="1.11.1.6"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_017933045.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_function: GO:0004096 - catalase activity [Evidence
                     IEA];
                     GO_function: GO:0020037 - heme binding [Evidence IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="catalase"
                     /protein_id="HOQ53338.1"
                     /translation="MSTKPPVPGAPSPQPPAAAEPVQPTGPLPPANDQRGPDPHTPTG
                     APVPLPGTAFAQQGSRLTSAGGTPLRDTDHSLKAGRRGPVLLADQHLREKINHFDHER
                     IPERVVHARGAAAHGTFVANGSAAGICAAAFLQAGVQTPVFVRFSTVLGSRGSADTVR
                     DTRGFATKFYTDEGVYDLVGNNIPVFFIQDGIKFPDIVHAAKPHPSREIPQAQSAHDT
                     FWDFVSLHTEAQAHTIWNMSDRGIPRSLRMMEGFGVHTFRLINAEGETSLVKFHWKPR
                     AGVHGLVWEEAQLLGGLDPDFHRRDLADAIEAGNYPQWDLGVQVMPDTDDETFEGIDL
                     LDPTKFVPEELCPVQIIGTMTLTANPVNYFAETEQVAFQVGNLVPGIDVTNDPLLQVR
                     LFSYLDTQMTRLGGPNFTQLPINRSHAPINDLLRDGMHQGAVHGGVAPYHPNTLDGGC
                     PFVVTEGAFIEAPRIPQTSERVRANHVTFQDHFSQAGLFFRSLSAVERDHVIAAYTFE
                     LSKCYELVIRQRQLQILTLVDADLAAGVAQGLGIPVPPKPDPGPDAVVPEPDGSTSPA
                     LSMVRGSWPVAGRTVGVVIGAGAHDNLSGLLDGLRAHQVLPLVIGPAGSSVNGELPQR
                     SYATATSVELDALVIAAPTPPEPGAQPSLDANAAAGAASDVDPRVAELLKQAWRHAKA
                     IGALSAGQSVLDAVGIPAAPGVVTAEATNLVTELTGLLATHRVWQRFAPVSAEVVR"
     gene            complement(3215..3655)
                     /locus_tag="PLF56_06960"
     CDS             complement(3215..3655)
                     /locus_tag="PLF56_06960"
                     /inference="COORDINATES: protein motif:HMM:NF022072.2"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="SRPBCC family protein"
                     /protein_id="HOQ53339.1"
                     /translation="MARSYYSTVVDAPADRVWSVVRDFNGLSTWWSSNVSESHIENGR
                     TGDSVGAVRSFAFGPDRIREHLLELSDQDRRCVYEFCPPPPFPVTGYVATLVVTPITD
                     GDRSFVEWWAHFDCAADELDHWTRFFAAEVFGPAVGSLKEFLAR"
     gene            complement(3696..4523)
                     /locus_tag="PLF56_06965"
     CDS             complement(3696..4523)
                     /locus_tag="PLF56_06965"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_016455351.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="phosphoenolpyruvate hydrolase family protein"
                     /protein_id="HOQ53340.1"
                     /translation="MSRQEILDRFRAQVAAGTPIVGGGAGTGITAKSAEAGGIDLLVI
                     YNSGRFRMAGRGSLSGLLAYGDANAIVMEMANEVLPVVKHTPVVAGVNGTDPFRVMPY
                     FLKQIQEIGFAGVQNFPTVGLIDGVFRANLEETGMGFGPEIDMIRTAHEMDLLTTPYA
                     FDADQAKDLAAAGADILVPHMGLTTSGTIGAQTALTLEEAAVKVQEIADAAKSVNPDI
                     LCLCHGGPIANPEDAQYILDHTEGIVGFYGASSIERFPTEVGIRKQTEDFKAITFRH"
     gene            complement(4618..>5309)
                     /locus_tag="PLF56_06970"
     CDS             complement(4618..>5309)
                     /locus_tag="PLF56_06970"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_015069171.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=3
                     /transl_table=11
                     /product="Tm-1-like ATP-binding domain-containing protein"
                     /protein_id="HOQ53341.1"
                     /translation="NRTESVHKPVIGMSMFGVTTPACDEARETLEALGYEVLVFHATG
                     TGGMALEKLVESGLLSGVCDLTTTELADDLVGGVLSAGPDRLKVAGATGVPQVVSLGA
                     LDMVNFGPLDTVPTQFAERNLYVHNPAVTLMRTTTAEMQELGRRIARKLAAATGPTEL
                     FIPLRGVSAIDVDGAPFRDAEADDALFTALRDGLADSAVVVHEVDQAINDPGFGKAVA
                     EALDQQITHAR"
BASE COUNT          810 a         1725 c         1899 g          875 t
ORIGIN      
        1 tacaggctgc tgaagccgaa caggctcgca cccttgccct gcgcggtgtt ctgggtggtc
       61 tcgcactggg cgaacagccc ccgcgaacac atccagcagt ggccgcacga gatgttgaac
      121 ggcaccacca cccggtcgcc gacgtgcaga ttgccgacct cgtcgccgac ctgttcgacg
      181 atgcccatga actcgtggcc gagcacgtcg cccttggtca ggaacggtgc gagcacgccg
      241 tagaggtgca ggtccgaccc gcagatcgca gtggacgtga cccgcacgac ggcgtccgtc
      301 ggctgctcga tggacgggtc gggcacgctg tcgacgcgca cgtcctcgac gccctgccag
      361 gtcaacgcct tcatcggacg acctcggccg agaccggcgc gaaccgctgc cacacccgat
      421 gcgtggcgag cagcccggtc agttcggtga ccaggttggt tgcctcggcc gtgacgactc
      481 ccggcgcggc gggaatgccg accgcgtcga ggacgctctg gccggcgctc agtgcaccga
      541 tcgccttggc atgtcgccag gcctgcttga gcaactcggc gaccctgggg tcgacgtcgg
      601 acgcggctcc cgccgccgca ttggcgtcca ggctcggctg ggcgccgggc tcgggcggag
      661 tgggcgcggc gattacgagc gcgtccagtt cgacggacgt ggcggtggcg tagctgcgct
      721 gcggcagctc gccgttgacg ctgctaccgg ccggtccgat gaccagcggc agcacctggt
      781 gggcccgcag cccgtccagc agtccggaca ggttgtcgtg tgcgccggcg ccgatcacca
      841 cgccgacggt gcggccggcc accggccatg atccgcgcac catcgacagt gccggggagg
      901 tgctgccgtc cggctcgggt acgacggcat cggggcccgg gtcgggcttc ggcggcaccg
      961 ggatgccgag gccctgcgcg accccagcgg cgaggtcggc gtcgaccagg gtcagaatct
     1021 gcaattgccg ctgcctgatc accagctcgt agcacttgct caactcgaag gtgtacgcgg
     1081 cgatgacgtg gtcgcgctcg accgcgctca gtgagcggaa gaacaagccg gcctggctga
     1141 agtgatcctg gaacgtcacg tggttggcac gcacccgctc ggacgtctgc ggaatgcgcg
     1201 gcgcctcgat gaacgccccc tcggtgacca cgaacgggca accgccgtcc agggtgttgg
     1261 ggtggtaggg cgccacgccg ccgtgcaccg cgccctggtg catgccgtcg cgcagcaggt
     1321 cgttgatcgg cgcgtggctg cggttgatcg gcagctgggt gaagttgggc ccgccgagcc
     1381 gggtcatctg ggtatcgagg tagctgaaca gccgcacctg cagcagcggg tcattggtga
     1441 catcgatacc cggcaccagg ttgccgacct ggaaggcgac ctgctcggtc tcggcgaagt
     1501 agttgaccgg gttggcggtc agcgtcatgg tgccgatgat ctggaccggg cacagctcct
     1561 cgggcacgaa cttggtcggg tcgagcaggt cgatgccctc gaaggtctcg tcgtcggtgt
     1621 cgggcatcac ctgcacgccc agatcccact gcgggtagtt gcccgcctcg atcgcgtcgg
     1681 ccaggtcgcg gcggtggaag tcggggtcga ggccgcccag caactgcgcc tcctcccaga
     1741 ccaggccgtg cacgccggcc ctcggcttcc agtggaactt gaccaggctg gtctcaccct
     1801 cggcgttgat caaccggaag gtgtgcacgc cgaagccctc catcatgcgc agcgaacgcg
     1861 gaatgccccg gtcggacatg ttccagatgg tgtgggcctg cgcttcggtg tgcagcgaca
     1921 cgaagtccca aaaggtgtcg tgcgccgatt gggcctgcgg gatctcgcgg ctggggtgcg
     1981 gcttggcggc atggacgatg tcaggaaact tgatgccgtc ctgaatgaag aacaccggaa
     2041 tgttgttgcc gaccaggtca tacacgcctt cgtcggtgta gaacttggtc gcgaaaccgc
     2101 gggtgtcgcg cacggtgtcg gccgagccgc gcgaacccag cacggtcgag aaccggacga
     2161 acaccggcgt ctgcacgccg gcctgcagga acgccgcggc gcagatgccc gcggccgagc
     2221 cgttggccac gaacgtgccg tgcgcagccg ccccacgggc gtgcaccacc cgctcgggga
     2281 tgcgttcgtg gtcgaagtgg ttgatcttct cgcgcagatg ctgatcggcc agcagcaccg
     2341 gcccgcgacg tccggccttc aaggagtggt cggtgtcgcg cagcggcgtg ccgccggccg
     2401 aggtcagccg ggacccctgc tgggcgaagg cggtgccggg cagcgggacc ggcgcgccgg
     2461 tcggggtgtg tgggtcggga ccgcgctggt cgttggcggg cggcagcggc ccggtgggct
     2521 ggacgggctc ggctgctgcg ggcggttgcg ggctgggtgc gccggggacc ggaggcttgg
     2581 ttgacatctt ccgagactgc ccctccacag ggaaacccaa cacacactct tgggtgaaag
     2641 gaggcatgcg ggagccggcg ggccgctggc gattgcgtgt cggggtaggt gcttgtggaa
     2701 atctgggctc tctgcggacg ggtggtgtgg ggtctctacc accgcgacca gcgaggcgtg
     2761 acctgagagg ggggcggttc cgaactcagg tcaggtggct ggttgggtct cgacaagccc
     2821 gaccagcgga cggggcccga cgctcgacgg gcgatgggta cgaactcgac ccccggtgag
     2881 ttctcgaccc cctgagttct cgaccccgca gttctcgact actgggtgct cgactactcg
     2941 tgcctgatcg gccgcgaaac tgcaccacat ttggggggcg tctcgcctcg gtgagcgatg
     3001 cagtggacga aatgtggtgc agcttcgcac accagccgag cgctccaccc gggagagcag
     3061 ccgtccagac atcgatcagc ctgccaggtg gcgacgtcca accaggcagg ggcgggtagg
     3121 gcggcggatc gaaacccggt ggtctgtgtt cgtccggggg tcgtccggtc gcacggaacg
     3181 catcagctga gcctggcccc cgcccagcgc cgactcacct cgccaagaac tctttgaggc
     3241 tgccgacggc ggggccgaac acttcggcgg cgaagaacct cgtccagtgg tcgagttcgt
     3301 cggctgcgca gtcgaaatgg gcccaccact cgacgaacga acggtcgccg tcggtgatcg
     3361 gggtcacgac cagcgtggcc acgtagccgg tgaccgggaa cggcggtggt gggcagaact
     3421 cgtagacgca gcggcggtcc tggtcggaca gttcgagcag gtgctcgcgg atgcggtccg
     3481 gcccgaacgc gaacgagcgg accgcgccca cggagtcgcc ggtccgtccg ttctcgatgt
     3541 gactctcgga cacgttcgac gaccaccagg tggagagccc gttgaagtcg cgcacgaccg
     3601 accacacccg gtcggccggc gcatcaacga cggtgctgta gtaggaacgg gccatggttc
     3661 tcctctcaga gtttgcggcg gaaggggaca ggtcgtcagt gccggaacgt gatcgccttg
     3721 aagtcctcgg tctgcttgcg gatgccgacc tcggtgggga accgctcgat cgaggacgcg
     3781 ccgtagaagc cgacgatgcc ctcggtgtga tcgaggatgt actgggcgtc ctcggggttc
     3841 gcgatcggcc cgccgtggca caggcacagg atgtcggggt tgaccgactt ggctgcgtcc
     3901 gcgatctctt gcaccttgac cgcggcctct tcgagggtga gggccgtctg tgccccgatc
     3961 gttccggacg tggtcagccc catgtgaggc accaggatgt cggctccggc cgcagccagg
     4021 tccttcgcct gatcggcgtc gaaggcgtac ggcgtggtca gcaggtccat ctcgtgggcg
     4081 gtgcggatca tgtcgatctc gggaccgaag cccatgccgg tctcttccag gttggcccgg
     4141 aagacgccgt cgatcaggcc gacagtcgga aagttctgca cgccggcgaa gccgatctct
     4201 tggatctgct tcaagaagta gggcatcacc cggaacgggt cggtgccgtt cacgccggcc
     4261 accacggggg tgtgcttgac caccggcagc acctcgttgg ccatctccat cacgatcgcg
     4321 ttcgcgtcgc cgtaggccag caggcccgag agcgacccgc gtccggccat tcggaaccgg
     4381 cccgagttgt agatgaccag caggtcgatg ccgccggcct cggccgactt ggccgtgatg
     4441 ccggtgccgg cgccgccgcc gacgatgggt gtgcccgctg cgacctgtgc gcggaaccgg
     4501 tccagaattt cctgacgaga catggtgggt gacctttcgt tgattgcatg tgatcggacg
     4561 cgcgtcgtgc ccggcttggg cgcggacgtc gtcctggttg aaatcggtgc ccgcgggtca
     4621 gcgggcgtgg gtgatctgct ggtcgagcgc ctcggcgacc gcctttccga agcccgggtc
     4681 gttgatcgcc tgatcgacct cgtgcacgac gaccgcgctg tcggcaagcc cgtcgcgaag
     4741 ggctgtgaac agtgcgtcgt cagcctcggc gtcgcggaac ggcgcaccgt cgacgtcgat
     4801 cgcgctgacc ccgcgcagcg gaatgaacag ctccgtcggc ccggtcgcgg cggccagttt
     4861 gcgggcgatc cggcgcccga gttcctgcat ctctgccgtg gtggttcgca tcagcgtgac
     4921 cgcggggttg tgcacgtaca ggttgcgttc ggcgaactgc gtgggcacgg tgtcgagcgg
     4981 gccgaagttc accatgtcga gcgcgcccag gctgaccact tgtggcaccc ccgtggctcc
     5041 ggcgacctta agccgatcgg gcccggccga cagcacgcca ccgacgaggt cgtcggccaa
     5101 ctctgtggtg gtgaggtcgc acaccccgga gagcaaaccg gattcgacca gcttctccaa
     5161 agccatacct ccggtgccgg tggcgtgaaa gaccagcacc tcgtagccca gcgcctccag
     5221 ggtctcgcgc gcctcgtcgc aggccggcgt ggtcacgccg aacatggaca taccgatgac
     5281 cggtttgtga acgctctcgg tgcggttct
//
LOCUS       DAOSUG010000176         5295 bp    DNA     linear   ENV 10-JUN-2024
DEFINITION  MAG TPA_asm: Micropruina sp. isolate AMR_MDS_2195
            NODE_6966_length_5295_cov_4.232252, whole genome shotgun sequence.
ACCESSION   DAOSUG010000176 DAOSUG010000000
VERSION     DAOSUG010000176.1
DBLINK      BioProject: PRJNA925477
            BioSample: SAMN32864262
            Sequence Read Archive: SRR4236673
KEYWORDS    WGS; ENV; Metagenome Assembled Genome; MAG; Third Party Data; TPA;
            TPA:assembly.
SOURCE      Micropruina sp. (wastewater metagenome)
  ORGANISM  Micropruina sp.
            Bacteria; Bacillati; Actinomycetota; Actinomycetes;
            Propionibacteriales; Nocardioidaceae; Micropruina.
REFERENCE   1  (bases 1 to 5295)
  AUTHORS   Abdulkadir,N., Saraiva,J.P., Zhang,J., Stolte,S., Gillor,O.,
            Harms,H. and Rocha,U.
  TITLE     Genome-centric analyses of 165 metagenomes show that mobile genetic
            elements are crucial for the transmission of antimicrobial
            resistance genes to pathogens in activated sludge and wastewater
  JOURNAL   Microbiol Spectr 12 (3), e0291823 (2024)
   PUBMED   38289113
REFERENCE   2  (bases 1 to 5295)
  AUTHORS   Abdulkadir,N.
  TITLE     Direct Submission
  JOURNAL   Submitted (24-MAR-2023) Environmental Microbiology, Helmholtz
            Center for Environmental Research -UFZ, Permoserstr 15, Leipzig
            04318, Germany
COMMENT     The annotation was added by the NCBI Prokaryotic Genome Annotation
            Pipeline (PGAP). Information about PGAP can be found here:
            https://www.ncbi.nlm.nih.gov/genome/annotation_prok/
            
            ##Genome-Assembly-Data-START##
            Assembly Method        :: SPAdes v. 3.11.1
            Genome Representation  :: Full
            Expected Final Version :: Yes
            Genome Coverage        :: Not Applicable
            Sequencing Technology  :: Illumina MiSeq
            ##Genome-Assembly-Data-END##
            
            ##Genome-Annotation-Data-START##
            Annotation Provider               :: NCBI
            Annotation Date                   :: 05/04/2023 04:57:10
            Annotation Pipeline               :: NCBI Prokaryotic Genome
                                                 Annotation Pipeline (PGAP)
            Annotation Method                 :: Best-placed reference protein
                                                 set; GeneMarkS-2+
            Annotation Software revision      :: 6.5
            Features Annotated                :: Gene; CDS; rRNA; tRNA; ncRNA
            Genes (total)                     :: 2,776
            CDSs (total)                      :: 2,750
            Genes (coding)                    :: 2,714
            CDSs (with protein)               :: 2,714
            Genes (RNA)                       :: 26
            tRNAs                             :: 24
            ncRNAs                            :: 2
            Pseudo Genes (total)              :: 36
            CDSs (without protein)            :: 36
            Pseudo Genes (ambiguous residues) :: 0 of 36
            Pseudo Genes (frameshifted)       :: 2 of 36
            Pseudo Genes (incomplete)         :: 32 of 36
            Pseudo Genes (internal stop)      :: 2 of 36
            ##Genome-Annotation-Data-END##
FEATURES             Location/Qualifiers
     source          1..5295
                     /organism="Micropruina sp."
                     /mol_type="genomic DNA"
                     /submitter_seqid="NODE_6966_length_5295_cov_4.232252"
                     /isolate="AMR_MDS_2195"
                     /isolation_source="Activated sludge of municipal
                     wastewater treatment plants"
                     /db_xref="taxon:2737536"
                     /environmental_sample
                     /geo_loc_name="Canada"
                     /lat_lon="49.25 N 123.1 W"
                     /collection_date="2015-06-16"
                     /metagenome_source="wastewater metagenome"
                     /note="metagenomic"
     gene            complement(183..488)
                     /locus_tag="PLF56_06975"
     CDS             complement(183..488)
                     /locus_tag="PLF56_06975"
                     /inference="COORDINATES: ab initio
                     prediction:GeneMarkS-2+"
                     /note="Derived by automated computational analysis using
                     gene prediction method: GeneMarkS-2+."
                     /codon_start=1
                     /transl_table=11
                     /product="hypothetical protein"
                     /protein_id="HOQ53342.1"
                     /translation="MSGPPPSFQYQVPPPQPGGGSGWLGAGIVAAVLALGFVLASSSR
                     SELGIGILLLGLPLLLVAGLVMVAIPATRRTGIGILITVGAGVLILGGVCVALLAAH"
     gene            complement(485..1243)
                     /locus_tag="PLF56_06980"
     CDS             complement(485..1243)
                     /locus_tag="PLF56_06980"
                     /EC_number="2.4.99.-"
                     /inference="COORDINATES: protein motif:HMM:NF013915.2"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_component: GO:0005886 - plasma membrane [Evidence IEA];
                     GO_function: GO:0008961 -
                     phosphatidylglycerol-prolipoprotein diacylglyceryl
                     transferase activity [Evidence IEA];
                     GO_process: GO:0042158 - lipoprotein biosynthetic process
                     [Evidence IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="prolipoprotein diacylglyceryl transferase"
                     /protein_id="HOQ53343.1"
                     /translation="MVPLPFAGSALVHGLFLGLGLLAGLGVFALELRRRGVHDERLWA
                     VAATALAMGAIGSRVGTWWQQFDPAQNESLLQWWNDGNRSVLAGLVGAWIGVLIGKRI
                     TGYRASTGDLFAPAVALAMAIGRIGCLLTELPGRPTGGDWGVVLTPQQASLLGGPAGV
                     GLQPTFLYEILFQAAAFGWLLRNRDRLAGADDLFVCYVAGYALFRFAVEFTRANEQVW
                     WGLTRPQWFLLLMAPLLVRRLVQVRQRTLIGATA"
     gene            1582..2484
                     /locus_tag="PLF56_06985"
     CDS             1582..2484
                     /locus_tag="PLF56_06985"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_017934401.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="hypothetical protein"
                     /protein_id="HOQ53344.1"
                     /translation="MTSTIDRVLPCFTNAAVVIAAPGAGPGNWAGAPAAIVSDGTYWL
                     AYRVRRPLTSGRGVATIIARSTDGEHFESVAELPREQFSAESFERPALVRLADGRWRL
                     YVSCATPGSKHWWVEAIDADTPEQLASGERRVVLPGSDTVAVKDPVIVRDGDDWHLWL
                     CCHPLDVAGAEDRMTTRYLTSHDGLSWHDHGLALAPDGTGWDARGTRVTAALSLDPPV
                     VLYDGRADAASNWFERTGLARGTGDGVRLEPVGDGPVAWSPESDGALRYVTAVPLPDG
                     RTRFYFEAARADGSHDLMTCVQSS"
     gene            complement(2521..2742)
                     /locus_tag="PLF56_06990"
     CDS             complement(2521..2742)
                     /locus_tag="PLF56_06990"
                     /inference="COORDINATES: ab initio
                     prediction:GeneMarkS-2+"
                     /note="Derived by automated computational analysis using
                     gene prediction method: GeneMarkS-2+."
                     /codon_start=1
                     /transl_table=11
                     /product="hypothetical protein"
                     /protein_id="HOQ53345.1"
                     /translation="MTVAAPLVERVWAALNGRVTPLDGRRVEVQEVETASIQGGSLTR
                     VTGVHARTKLRHLYALLIKRVGAALTKGV"
     gene            complement(2837..3805)
                     /locus_tag="PLF56_06995"
     CDS             complement(2837..3805)
                     /locus_tag="PLF56_06995"
                     /EC_number="2.8.2.-"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_013865696.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_function: GO:0008146 - sulfotransferase activity
                     [Evidence IEA];
                     GO_function: GO:0050656 - 3'-phosphoadenosine
                     5'-phosphosulfate binding [Evidence IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="sulfotransferase"
                     /protein_id="HOQ53346.1"
                     /translation="MSTPRTPQPDALLIGAPKAGTSALHAALALHPGVFTSTPKEPKY
                     YLCADAPPPAYCGPGDAHSQREWIWRYEDYQRLFEPARPDQLRIESTPFYLYLRNARR
                     RIAEELPNVKLIAVIRDPVDRAYSNWMHLWSDGLEPVDDFPTAFHLEQERIAKGWAPF
                     WHYRRQGFYGRQLDDLYRHVDADRILVVRYRELVDDPSGLLDRVSAFLGLEPGVISTI
                     PRENSRPYVAPSATTSVLARMVRAGAAAGAWFPPQVWRRASQPLTGLMQSRGAAARPR
                     LDPAVRAQLVDEFSDDIDLLEQVTGRSFSDWRSNVGAGSFAARTAG"
     gene            complement(4024..>5295)
                     /gene="groL"
                     /locus_tag="PLF56_07000"
     CDS             complement(4024..>5295)
                     /gene="groL"
                     /locus_tag="PLF56_07000"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_002549889.1"
                     /note="60 kDa chaperone family; promotes refolding of
                     misfolded polypeptides especially under stressful
                     conditions; forms two stacked rings of heptamers to form a
                     barrel-shaped 14mer; ends can be capped by GroES;
                     misfolded proteins enter the barrel where they are
                     refolded when GroES binds; Derived by automated
                     computational analysis using gene prediction method:
                     Protein Homology.
                     GO_function: GO:0005515 - protein binding [Evidence IEA];
                     GO_function: GO:0016887 - ATP hydrolysis activity
                     [Evidence IEA];
                     GO_process: GO:0006457 - protein folding [Evidence IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="chaperonin GroEL"
                     /protein_id="HOQ53347.1"
                     /translation="ANPVGLKRGIEAAVEAVSDKLRELAREVDDTDDMANVATISARD
                     AEIGRLIADAFDKVGKDGVITVDESQTFGTELEFTEGMQFDKGYLSPYFVTDPERMEA
                     VLEDPYILINQGKISSMNDLLPLLEKVIGAGGTLFIVAEDVDGEALSTLVVNKIRGTF
                     TSVAVKAPAFGDRRKAMLEDIAILTGGKVVAPEVGLKLDQVGLDVLGRARRIVVTKDN
                     TTIVDGAGSHEEVSGRVAQLHAEIARTDSDWDREKLQERVAKLAGGVCVIKVGAATEV
                     ELKEKKHRIEDAVSATRAAIEEGIVAGGGSALVHAARVLEGDLGKTGDEAAGVRIVAK
                     SVVEPLRWIAENGGEPGYVIVSKVKELEPGNGYNAATGEYVDLLAAGVIDPVKVTRSA
                     LANAASIAALLLTTETLVVEKPEDEEDGHNH"
BASE COUNT          835 a         1815 c         1816 g          829 t
ORIGIN      
        1 cctggtgcgg ttgtgagttg cttcgattgc ggtttggttc ggctgttgaa gtgttcggct
       61 gtgatctgct tcggttgcga tctgctgcgg ttgcgttttg gttcaggagc gacctggtgc
      121 ggttacgact tggttcgggt gcggccgggt tctgatcgtc gcctggttcg ggagcgctgc
      181 gttcagtggg cggccagcag cgcgacgcag acgccgccca ggatgagcac ccccgcgccg
      241 acagtgatca ggatgccgat ccccgtgcgg cgggtggccg ggatcgcaac catcaccaac
      301 ccggcgacca gcagcagcgg caagccgagc aacaagatgc cgatgccgag ttctgacctc
      361 gatgaggatg ccaggacgaa gccgagcgca aggacagccg cgacgatgcc ggcgccgagc
      421 cacccggaac caccgccggg ctgcgggggc gggacctgat actgaaacga tggcggtgga
      481 ccgctcatgc tgtcgctccg atcaatgtgc gttgcctcac ctgcaccagc cgccggacca
      541 gcagcggcgc catcagcagc agaaaccact gtggccgggt caatccccac cacacctgct
      601 cgttggcccg ggtgaactcg acggcgaacc ggaacaatgc gtagccggcg acgtaacaca
      661 cgaacaggtc gtcggccccg gccagccgat cgcggttgcg cagcagccag ccgaaggctg
      721 ccgcctgaaa gaggatttcg tacaggaatg tgggttgaag accgacgccg gcggggccac
      781 cgagcagcga cgcctgttgc ggggtcagca cgacgcccca gtcaccgccg gtgggacgtc
      841 cgggcaactc ggtgagcagg cagccgatcc gtccgatcgc catcgccagc gcgacggcgg
      901 gagcgaagag gtcgccggtc gaagctcggt agccggtgat ccgcttgccg atcagcacgc
      961 cgatccaggc accgaccagg ccggccagca cgctgcgatt accgtcgttc caccactgca
     1021 gtagcgattc gttctgtgcc gggtcgaact gttgccacca ggtgccgacc cgcgaaccga
     1081 tggcacccat cgcaagggcg gtagccgcta ccgcccagag tcgctcgtcg tgcaccccgc
     1141 ggcgccgcaa ctcgagggcg aacaccccca ggccagccaa gaggccaagg cccaggaaaa
     1201 gcccgtgcac cagtgcgctg ccggcgaacg gaaggggcac catgcgcaca cggtagccgg
     1261 atgcggctcg acgcggcggc acacgtacga tctggcggcc ggacaggggt ggcacacgta
     1321 cgatctgccg gccggacagg ggtggcacac gtacgatctg gcggccggaa ggcggcggca
     1381 cagacacggt tctcattcct gcccaacgcg tgcccggcga ggacgaaccc agcggccaag
     1441 gggtgagctg gtgcgccatc tgtgacggcg cccccaacgc gacgcgggtt cgctccgtcc
     1501 cagcggggaa cctccgcccc ggcgcttgat cgaagagcgg aatgccacgg ggctcgccgt
     1561 attcaccgta gtcttccgct catgacttcg acgatcgacc gcgtgctgcc ctgcttcacc
     1621 aacgccgcgg tcgtcatcgc agcccccggt gccgggcccg gcaactgggc gggggcgcct
     1681 gcggccatcg tcagcgacgg cacctactgg ctggcctacc gggtgcgccg tcccctcacc
     1741 tcgggacggg gggtcgccac catcatcgcc cgctccaccg acggcgagca cttcgaatcg
     1801 gtcgcggagt tgccgcggga gcagttcagt gccgaatcgt tcgagcgtcc cgcgctggtt
     1861 cgcctcgccg atggacgctg gcggctctac gtctcgtgcg ccacgcccgg ttcgaagcac
     1921 tggtgggtcg aggcgatcga cgcggacacc cccgagcaac tggcgtccgg tgagcgccgg
     1981 gtggtgctgc cgggcagcga cacggttgcg gtcaaggatc ccgtgatcgt gcgtgacggc
     2041 gacgactggc acctgtggct gtgctgccac cccctggacg tcgccggtgc cgaagaccgc
     2101 atgacgaccc gctacctgac cagccacgac gggctgagct ggcacgacca cgggctcgcc
     2161 ctggcaccgg acggcacggg ctgggacgcc cgcggcaccc gggtgaccgc ggcgctgagt
     2221 ctcgacccgc cggtggtgct ctacgacggc cgggcggacg cggcgtccaa ctggttcgag
     2281 cgcacgggac tggcccgcgg caccggcgac ggagtgcggc tggagccggt cggcgacgga
     2341 cctgttgcct ggtcacccga atcggacggg gccctgcgct acgtcaccgc ggttccgctg
     2401 cccgatgggc gtacccggtt ctatttcgag gccgctcgcg ccgacgggtc ccacgacctg
     2461 atgacctgcg tacagtcgtc ctgaagcctc cactcggcag ccgcccgggc gagcgcgtcg
     2521 tcacactccc ttggtgaggg cggccccgac acgcttgatc agtaacgcgt acaggtgacg
     2581 gagtttggtg cgggcgtgca ctccggtcac ccgggtgaga ctgccgccct gaatcgacgc
     2641 cgtctcgacc tcctgaacct cgacgcggcg tccgtcgagc ggcgtcacac gaccgttgag
     2701 ggccgcccag acacgctcga ccagcggcgc ggctacggtc acccgggcga gactgttccc
     2761 acccctgaga atcgatacgg tttcgaaccc gcccgatgaa cgaccggtcg ggccatcaac
     2821 gaccgggcgg gccgggtcaa ccggccgtcc gggccgcgaa cgagccggcg ccgacgttcg
     2881 agcgccagtc gctgaacgac cgtccggtga cctgctcgag caggtcgata tcgtcgctga
     2941 actcgtcgac caactgtgcc cgcaccgccg ggtcgagcct cggacgggcg gccgcacctc
     3001 gggattgcat cagcccggtc aggggttggc tcgcgcgtcg ccacacctgc ggggggaacc
     3061 acgcgccggc cgccgccccg gcacgcacca tccgcgcaag tacggacgtc gtggcgctgg
     3121 gcgcgacgta cggccgcgag ttctcgcgcg ggatcgttga gatcaccccc ggttcgaggc
     3181 caaggaacgc gctgacccgg tcgagcagcc cggacggatc gtccaccaac tcgcgatagc
     3241 gcaccaccag gatgcggtca gcgtcgacgt ggcggtacag atcgtccaac tgacgtccgt
     3301 agaagccctg ccggcgatag tgccaaaacg gcgcccagcc cttggcgatg cgttcctgtt
     3361 cgaggtggaa ggcggtcgga aagtcgtcga ccggctcgag cccgtccgac cagaggtgca
     3421 tccagttgct gtacgcccgg tcgaccgggt cacggatcac cgcgatcagc ttgacgttcg
     3481 gcaactcttc cgcgatccgg cggcgggcgt tgcgcaggta aaggtagaag ggtgtgctct
     3541 cgatgcgcaa ctgatcgggc cgggcgggtt cgaacagccg ctggtagtcc tcgtagcgcc
     3601 agatccattc gcgctggctg tgtgcgtcac cggggccgca gtaggccggc ggcggagcgt
     3661 ccgcgcacag gtagtacttc ggttccttgg gggtgctggt gaacaccccg gggtggagcg
     3721 ccagggctgc gtgcagggcg ctggtgcctg ctttcggagc accgatcagc agggcgtccg
     3781 gctggggtgt gcggggcgtc gacatgccgg aaatgctagg ggctgagcgg cggcatctcc
     3841 caccggttca ctatgtgtta gcgccgaccg ctcccctaac catcgtgggc ctacgtagtt
     3901 gcgcaggcag aagatggtta ggggggcgct cggcgaaccg cgctgcggcc ccgcacacca
     3961 cgaagccccc cacccggacg ggcagggggc ttcgttcagc ggtcgtacga aaccggtacg
     4021 acctcagtgg ttgtgaccgt cctcttcgtc ctccggcttc tcgaccacca gggtttcggt
     4081 ggtgagcagc agggcggcga tggaggcggc gttcgccagc gcggagcgcg tcaccttgac
     4141 cgggtcgatg acgccggcgg ccagcaggtc gacgtattcg ccggtggcgg cgttgtaccc
     4201 gttgccaggc tccaactctt tcaccttcga cacgatcaca tagcccggct cgccgccgtt
     4261 ctcggcgatc cagcgcagcg gctcgaccac cgacttggcc acgatgcgga cgcccgcggc
     4321 ctcgtcaccg gtcttaccca ggtcaccctc cagcacccgt gcagcatgga cgagagcgct
     4381 gccgccaccg gcgacgatgc cctcttcgat ggccgcccgg gtcgcggaca cggcgtcctc
     4441 gatgcggtgc ttcttctcct tgagctcgac ctcggtcgcg gcaccgacct tgatcacgca
     4501 gacgccaccg gcgagcttgg cgacccgctc ctgcagcttc tcgcggtccc agtcggaatc
     4561 ggtgcgggcg atctcggcat gcagctgagc cacccgtccg gagacctctt cgtgcgaccc
     4621 ggccccgtcc acgatcgtgg tgttgtcctt ggtgaccacg atgcgccggg cacggcccag
     4681 cacgtcgagg ccgacctggt cgagcttcag cccgacctcg ggcgcgacga ccttgccacc
     4741 ggtgagaatg gcgatgtctt cgagcatggc cttgcggcga tcaccgaagg cgggcgcctt
     4801 gacggccacc gaggtgaagg tgccacggat cttgttcacc acgagggtgg agagggcttc
     4861 gccgtccacg tcctcggcca caatgaacag ggtgccaccg gccccgatga ccttctcgag
     4921 caagggcagc aggtcgttca tcgagctgat cttgccctga ttgatcagga tgtacgggtc
     4981 ctcgagcacg gcttccatgc gctcggggtc ggtcacgaag tacggcgaca ggtaaccctt
     5041 gtcgaactgc atgccctcgg tgaactcgag ttcggtgccg aaggtctgcg actcgtcgac
     5101 ggtgatcacg ccgtccttgc cgaccttgtc gaacgcgtcg gcgatcagcc ggccgatctc
     5161 ggcgtcgcgc gccgagatgg tggccacgtt ggccatgtcg tcggtgtcgt cgacctcgcg
     5221 ggccagctca cgcagcttgt cgctgacggc ctcgacggcc gcctcgatgc cacgcttgag
     5281 gccgaccggg ttggc
//
LOCUS       DAOSUG010000177         5211 bp    DNA     linear   ENV 10-JUN-2024
DEFINITION  MAG TPA_asm: Micropruina sp. isolate AMR_MDS_2195
            NODE_7073_length_5211_cov_3.155547, whole genome shotgun sequence.
ACCESSION   DAOSUG010000177 DAOSUG010000000
VERSION     DAOSUG010000177.1
DBLINK      BioProject: PRJNA925477
            BioSample: SAMN32864262
            Sequence Read Archive: SRR4236673
KEYWORDS    WGS; ENV; Metagenome Assembled Genome; MAG; Third Party Data; TPA;
            TPA:assembly.
SOURCE      Micropruina sp. (wastewater metagenome)
  ORGANISM  Micropruina sp.
            Bacteria; Bacillati; Actinomycetota; Actinomycetes;
            Propionibacteriales; Nocardioidaceae; Micropruina.
REFERENCE   1  (bases 1 to 5211)
  AUTHORS   Abdulkadir,N., Saraiva,J.P., Zhang,J., Stolte,S., Gillor,O.,
            Harms,H. and Rocha,U.
  TITLE     Genome-centric analyses of 165 metagenomes show that mobile genetic
            elements are crucial for the transmission of antimicrobial
            resistance genes to pathogens in activated sludge and wastewater
  JOURNAL   Microbiol Spectr 12 (3), e0291823 (2024)
   PUBMED   38289113
REFERENCE   2  (bases 1 to 5211)
  AUTHORS   Abdulkadir,N.
  TITLE     Direct Submission
  JOURNAL   Submitted (24-MAR-2023) Environmental Microbiology, Helmholtz
            Center for Environmental Research -UFZ, Permoserstr 15, Leipzig
            04318, Germany
COMMENT     The annotation was added by the NCBI Prokaryotic Genome Annotation
            Pipeline (PGAP). Information about PGAP can be found here:
            https://www.ncbi.nlm.nih.gov/genome/annotation_prok/
            
            ##Genome-Assembly-Data-START##
            Assembly Method        :: SPAdes v. 3.11.1
            Genome Representation  :: Full
            Expected Final Version :: Yes
            Genome Coverage        :: Not Applicable
            Sequencing Technology  :: Illumina MiSeq
            ##Genome-Assembly-Data-END##
            
            ##Genome-Annotation-Data-START##
            Annotation Provider               :: NCBI
            Annotation Date                   :: 05/04/2023 04:57:10
            Annotation Pipeline               :: NCBI Prokaryotic Genome
                                                 Annotation Pipeline (PGAP)
            Annotation Method                 :: Best-placed reference protein
                                                 set; GeneMarkS-2+
            Annotation Software revision      :: 6.5
            Features Annotated                :: Gene; CDS; rRNA; tRNA; ncRNA
            Genes (total)                     :: 2,776
            CDSs (total)                      :: 2,750
            Genes (coding)                    :: 2,714
            CDSs (with protein)               :: 2,714
            Genes (RNA)                       :: 26
            tRNAs                             :: 24
            ncRNAs                            :: 2
            Pseudo Genes (total)              :: 36
            CDSs (without protein)            :: 36
            Pseudo Genes (ambiguous residues) :: 0 of 36
            Pseudo Genes (frameshifted)       :: 2 of 36
            Pseudo Genes (incomplete)         :: 32 of 36
            Pseudo Genes (internal stop)      :: 2 of 36
            ##Genome-Annotation-Data-END##
FEATURES             Location/Qualifiers
     source          1..5211
                     /organism="Micropruina sp."
                     /mol_type="genomic DNA"
                     /submitter_seqid="NODE_7073_length_5211_cov_3.155547"
                     /isolate="AMR_MDS_2195"
                     /isolation_source="Activated sludge of municipal
                     wastewater treatment plants"
                     /db_xref="taxon:2737536"
                     /environmental_sample
                     /geo_loc_name="Canada"
                     /lat_lon="49.25 N 123.1 W"
                     /collection_date="2015-06-16"
                     /metagenome_source="wastewater metagenome"
                     /note="metagenomic"
     gene            complement(<1..280)
                     /locus_tag="PLF56_07005"
     CDS             complement(<1..280)
                     /locus_tag="PLF56_07005"
                     /inference="COORDINATES: protein motif:HMM:NF012301.2"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="response regulator transcription factor"
                     /protein_id="HOQ53348.1"
                     /translation="MRLLVADDAALIRAGLVGLLERAGHTVVAEAGDAPELLAQCRRL
                     AAGDGLPDLVITDVRMPPSMTDDGLRAAVQLRASYPGLPVLMVSQYIAP"
     gene            complement(430..1680)
                     /locus_tag="PLF56_07010"
     CDS             complement(430..1680)
                     /locus_tag="PLF56_07010"
                     /inference="COORDINATES: protein motif:HMM:NF019350.2"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_component: GO:0016020 - membrane [Evidence IEA];
                     GO_function: GO:0000155 - phosphorelay sensor kinase
                     activity [Evidence IEA];
                     GO_function: GO:0046983 - protein dimerization activity
                     [Evidence IEA];
                     GO_process: GO:0000160 - phosphorelay signal transduction
                     system [Evidence IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="histidine kinase"
                     /protein_id="HOQ53349.1"
                     /translation="MSEGYGHNDESPAGVRTTSPVHDPSAAARARLRPPFNPLAMLIS
                     PLGWGAVAYCASSIIGGLLALGAGILGILVLPLMTWCTATLESHRLRLLGLPFPGPLP
                     SGTMRHPWDPGGLKEGNLAVWGVAVLFAIVDLLPGLMLSLLVIGLWASLMRLVIGGHA
                     FGVDHFLVGTGLLVVLVVGLYVAWALAAVQALIVDHLVRPKVELTEQVEQLTTSRREL
                     VDVFAAERRRIERDLHDGAQQQLVLLSMQLGEADYALAQGRPDEAKLALAAAQQSMEH
                     AMVSLRETVRGVHPQILTDRGLVPAVHELAQRQPIPVTVIVERDGEPPEHVASAAYYL
                     ISEAFTNAAKHAGASSMVVRLDLGDPMVIEVQDDGRGGAQVRPGHGLSGMIERVQAFG
                     GRCSLSSPPGGPTSIRAMIPNPPG"
     gene            1811..2506
                     /locus_tag="PLF56_07015"
     CDS             1811..2506
                     /locus_tag="PLF56_07015"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_014847262.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_function: GO:0005524 - ATP binding [Evidence IEA];
                     GO_function: GO:0016887 - ATP hydrolysis activity
                     [Evidence IEA];
                     GO_function: GO:0042626 - ATPase-coupled transmembrane
                     transporter activity [Evidence IEA];
                     GO_function: GO:0140359 - ABC-type transporter activity
                     [Evidence IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="ABC transporter ATP-binding protein"
                     /protein_id="HOQ53350.1"
                     /translation="MHNNSGATLSAHRISKSFGEVRALDQVSLDIPAGQSVSVMGPSG
                     SGKSTLLHCLAGILRPDEGEVTLGGEPISLASDAARSRLRLQRMGFVFQDGQLLPELS
                     AQENVALPLLLLGRPRVEAFTRAGECLAWLGLAGLEGRRPGQLSGGQAHRVAIARALV
                     GRPSVVMADEPTGALDQATGAEVMRVLTSSVAAAGASLVVVTHDAQVAAWCRRHVEIV
                     DGRISSDLVSRAA"
     gene            2506..3864
                     /locus_tag="PLF56_07020"
     CDS             2506..3864
                     /locus_tag="PLF56_07020"
                     /inference="COORDINATES: ab initio
                     prediction:GeneMarkS-2+"
                     /note="Derived by automated computational analysis using
                     gene prediction method: GeneMarkS-2+."
                     /codon_start=1
                     /transl_table=11
                     /product="hypothetical protein"
                     /protein_id="HOQ53351.1"
                     /translation="MRPLSITPLLLRGRIRAGSGLIDLLGVLAFTISSWLLQLVLGGV
                     NMFVERQHNPPTEFVAALGSSAAQGIAELQVWTLLAACAGVLLVVPVVTLGAAAARMG
                     ALGRDQRLATLRLLGLSAGQAVALSTVETMLAALAGGVLGTVGYLLTLPLWSAVTFQA
                     TPLSAAAMLLPAWAIAATIAGLVVLAGLSSVTGLMRLRISPLGVARREPRPALRLWRF
                     AALPVVIVLWLAVAPALSLTRSFVYSATLIVIVLAMFMGVINLIGPWLLQLLGAMLAR
                     SGHPSVLLAGRRLQADAKGAWRNVSGLAFVGFTGGAMVSLPDFSARSGDPLLRILTDD
                     LRTGTYLTLAIAFVVAAASALLNQGAAVLDRRLELRQLANLGVPAALHDRTRLVEVIA
                     PAVLSAMGSGGLAVLFFAQLPRVGANTSGPWLFAGALVLGVALVWVAGEACRPLVRRA
                     VS"
     gene            complement(3895..4524)
                     /locus_tag="PLF56_07025"
     CDS             complement(3895..4524)
                     /locus_tag="PLF56_07025"
                     /inference="COORDINATES: protein motif:HMM:NF018745.2"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="DUF1345 domain-containing protein"
                     /protein_id="HOQ53352.1"
                     /translation="MNVHPAWVRSGAAAALGLIAAAVLWAPAGAAFAVAVGWIVLTLI
                     YVLWTGLATHRFTPEQTRTHALTEDASSGTVAVILTLAAVASILGVGILLAGARAQGP
                     VPVEAILGVAVVACSWALVHTVYAVHYARLYYLQAGDAIDFNGDQPDYHDFAYLAFTI
                     GMTYQVSDTNLNARVIRRAALHHALLSYLLGAVVLACTINLVVQLASQG"
     gene            complement(4533..>5211)
                     /locus_tag="PLF56_07030"
     CDS             complement(4533..>5211)
                     /locus_tag="PLF56_07030"
                     /inference="COORDINATES: ab initio
                     prediction:GeneMarkS-2+"
                     /note="Derived by automated computational analysis using
                     gene prediction method: GeneMarkS-2+."
                     /codon_start=2
                     /transl_table=11
                     /product="hypothetical protein"
                     /protein_id="HOQ53353.1"
                     /translation="EVNRSSGGALSADATLLTPATQAFSIWSVIYVGLTAYLIWQWLP
                     AHAHSRTARETGWWAAASLLLNGAWLGVTQAGWLWVSVLVIALLTYVLAKLCLTLVGR
                     GPNSLTELLIVYVTFGLYLGWVCVATVANVTAAAVGTGARLGEAAPVLAVVVLLVAGA
                     IGVLLARVMPGQPAIGVAMAWGLAWIAVGRLLVQPQSLIVGVVAALVAAGVLAAHVFG
                     ARVSVRG"
BASE COUNT          760 a         1859 c         1831 g          761 t
ORIGIN      
        1 cgggcgcgat gtactgactg accatcagta ccggcaggcc ggggtagctc gcgcgcagtt
       61 gcacggctgc ccgcagcccg tcatcggtca tggacggggg catgcgcacg tcggtgatca
      121 ccaggtcggg cagcccgtca cccgcggcca gccgccggca ctgggcgagc agctcgggcg
      181 catcgccggc ctcggcgacc accgtgtgtc ccgcccgttc gagcaacccc accagtccgg
      241 cccggatcag ggctgcgtca tcggccacca acaaccgcat ctggttccct cctcagggca
      301 gcacgttagc cctggtttga gccggcgcag gccagcgggg gccggtcacg accagcgcaa
      361 ccatcgagag gcgaatatcg agaggcgacc acccaggggc gggcaccatc cacacgctcg
      421 accgccccat catccgggcg ggttcggaat catcgctcgg atgctggtgg gcccacccgg
      481 tgggctgctc agcgagcacc gaccgccgaa tgcctgcacc cgttcgatca tgccgctgag
      541 gccgtgcccc ggacgtacct gcgcgccgcc gcgcccgtcg tcctgtacct cgatcaccat
      601 cgggtcgccc aggtcgagtc gcaccaccat cgaggacgca ccggcgtgct tggccgcgtt
      661 cgtgaacgcc tcgctgatca ggtagtacgc cgccgacgcc acgtgctcgg gaggctcgcc
      721 gtcccgttcg acgatcaccg tcaccgggat cggctgtcgt tgcgccagct catgcacggc
      781 cggcaccagg ccgcggtcgg tgagaatctg gggatgcacc ccgcgcactg tctcgcgcaa
      841 cgacaccatg gcgtgctcca tcgactgctg ggcggccgcg agggccagct tcgcctcgtc
      901 cggacggccc tgcgcgagcg cgtagtcggc ctcacccaac tgcatgctga gcagcaccag
      961 ctgctgctgc gcaccgtcgt gcaggtcgcg ttcgatgcga cgccgctcgg cggcgaacac
     1021 gtccaccagt tcgcgtcgtg aggttgtcag ttgctccacc tgctcggtca actcaacctt
     1081 cggccggacc agatgatcca cgatgagcgc ctgcacggcg gccaacgccc aggcgacgta
     1141 caggccgacg accagcacca ccagcagtcc ggtgccgacc agaaagtggt cgaccccgaa
     1201 tgcgtgaccg ccgatcacca gccgcatcaa cgacgcccac aggccgatca ccagcaagct
     1261 cagcatcagt ccgggcagca ggtcgacgat cgcgaacagc accgcaacgc cccataccgc
     1321 caggttgccc tctttgaggc cccccggatc ccacggatgc cgcatcgtgc cgctgggcag
     1381 tggacctgga aacggcaggc cgagcaggcg cagccgatgg ctctcgagcg ttgcggtgca
     1441 ccaggtcatc agcggcagca ccaggatgcc gagaatgccg gcgcccagcg ccagcaggcc
     1501 gccgatgatc gagctggcgc agtacgccac cgcaccccag cccagcggac tgatcagcat
     1561 ggccagggga ttgaacggtg gacggagccg tgcccgtgcc gccgcggacg ggtcgtgcac
     1621 cggcgaggtc gtccgcacgc cggccggcga ctcgtcgttg tgcccatagc cctcgctcac
     1681 cgctccacgc tacggggttc cgtacccctg ggcagtggtg ccagcactac cccccggtgg
     1741 tgtgtgcacc accgcagaac accggccagc gcccgtgtgg ccggcacccc gagccggtgg
     1801 aatggcaggc atgcacaaca actccggcgc caccctgagc gcccaccgca tctccaagtc
     1861 tttcggcgaa gtccgggccc tcgaccaggt cagcctcgac atacccgccg gccagtcggt
     1921 gtcggtgatg ggtccgtccg gctcgggcaa gtcgacactg ctgcactgcc tggcgggcat
     1981 tctccgtccg gacgaggggg aggtcaccct gggcggcgag ccgatctcgc tcgcctcgga
     2041 cgccgcccgc agccggttgc ggctgcagcg catgggcttc gtctttcagg acggtcaact
     2101 gctgcccgaa ttgtcggcgc aggagaacgt ggcgctgccg ctgctgctgc tcggacgtcc
     2161 gcgcgtcgag gccttcacgc gggcggggga gtgcctggcc tggttggggc tggccggcct
     2221 ggagggtcgc cggccggggc agctgtcggg tgggcaggcg caccgggtcg cgatcgcacg
     2281 cgccctggtc ggacgcccgt cggtggtgat ggcggacgaa cccacgggcg ccctcgacca
     2341 ggcgaccggc gccgaggtga tgcgcgtgct cacctcgtcg gtggccgcgg cgggtgccag
     2401 cctggtggtg gtcacccacg acgcgcaggt ggccgcctgg tgccggcggc acgtcgaaat
     2461 cgtcgacggc cggatcagca gcgacctggt gagcagggcc gcctgatgcg ccccctgagc
     2521 atcaccccgc tgctgctgcg gggcaggatc cgggccggca gcggtctgat cgacctactc
     2581 ggtgtgctgg ccttcacgat cagtagttgg ctgctgcagc tcgtgctggg tggcgtcaac
     2641 atgttcgtcg agcgtcaaca caatccgccc accgagttcg tcgccgcgct gggttcgagc
     2701 gcggcacagg gcattgccga actgcaggtc tggacgctgc tggccgcctg cgccggcgtg
     2761 ctgctggtgg tgcccgtggt gacgctcggc gccgctgcgg cccggatggg ggcgttgggt
     2821 cgcgatcagc ggctcgccac gctgcggctg ctcgggctca gtgccggcca ggccgtggcg
     2881 ctgagcactg tcgaaaccat gctggcggcc ctggccggcg gcgtgctcgg caccgtcggc
     2941 tacctgctca cgctgccgct gtggtcggcg gtcacgtttc aggcgacacc gttgagcgcc
     3001 gccgcgatgc tgctgcccgc ctgggccatc gcggcgacca tcgccggcct ggtggtgctg
     3061 gccggactga gttcggtgac cgggctgatg cggctgcgca tctcgccgct gggagtggcg
     3121 cggcgcgagc cccgtccggc gttgaggttg tggcgcttcg ccgcattgcc cgtggtgatc
     3181 gtgctgtggt tggcggtggc accggcgctg agcctgaccc gctccttcgt ctactcggcg
     3241 accctgatcg tgatcgtgct ggccatgttc atgggcgtga tcaacctgat cggcccctgg
     3301 ctgttgcaac tgctgggcgc gatgctcgcg cgctccggcc acccgtcggt gctgctggcc
     3361 ggacgccgac tgcaggccga cgccaagggc gcgtggcgca acgtgtcggg cctggccttc
     3421 gtcggcttca ccggcggagc aatggtcagc ctgcccgact tcagtgcgcg ttcgggcgat
     3481 ccgctgttac ggattctcac cgacgatctg cgtaccggaa cgtacctcac cctcgccatc
     3541 gcgttcgtgg tggctgccgc ctccgcgctg ctcaatcagg gggccgcggt gctcgatcgc
     3601 cgactcgaac tgcgccagtt ggccaacctc ggcgtgcccg ccgcgctgca cgaccgcacc
     3661 cggctggtcg aggtgatcgc gcccgccgtc ctgagtgcga tgggctcggg cggcctggcg
     3721 gtgctgttct tcgcccaact gcccagggtg ggcgccaaca cgtccgggcc gtggctgttc
     3781 gccggcgccc tggtgctggg ggtggcgctg gtctgggtcg ccggcgaggc gtgtcgtccg
     3841 ctggtgcgcc gggcggtgag ttgagccggg cggtcgagcc cggccggcgc cggctcagcc
     3901 ctgcgaggcc aactggacga ccaggttgat cgtgcaggcc agcaccaccg caccgagcag
     3961 gtacgacagc agcgcgtggt gcagggcggc ccgacggatc acccgggcgt tcaggttggt
     4021 gtcgctgacc tggtaggtca tgccgatggt gaacgccagg taggcgaaat cgtgatagtc
     4081 gggctgatca ccgttgaagt cgatggcgtc gccggcctgc aggtagtaca accgggcgta
     4141 gtgcactgcg tacacggtgt gcaccagcgc ccacgagcac gccaccaccg cgacgcccag
     4201 aatcgcctcg accggcacgg gtccctgggc tcgggcgccg gccagcagga tgcctacgcc
     4261 gagaatgctg gccacggcgg ccagggtcag gatcactgcg accgtgccgc tgctggcgtc
     4321 ctcggtgagg gcgtgcgtgc gggtctgctc cggggtgaag cggtgggtgg ccaggcccgt
     4381 ccagagcaca tagatgaggg tgagcacgat ccatcccacc gccacggcga aggcggcgcc
     4441 cgcgggcgcc cacaacaccg cggcggcgat cagcccgagc gcggcggctg ctccggagcg
     4501 aacccaggcg gggtggacgt tcacggcgtc agtcaaccac gcaccgacac ccgcgcaccg
     4561 aacacgtgcg cggcgagcac gccggccgcc accagtgcgg cgaccacacc aacgatcagc
     4621 gactggggct gcaccagcag ccgtccgacg gcgatccagg ccaggcccca ggccatggcg
     4681 acgccgatcg cgggctggcc cggcatcacc cgggccaaca gcaccccgat ggcgccggcc
     4741 accagcagca ccaccacggc gagcacgggg gcggcctcgc ccaaccgggc cccggtgccg
     4801 actgcggcgg cggtgacgtt ggcgacggta gccacgcaca cccagcccag gtagagcccg
     4861 aacgtgacgt agacgatcag cagctcggtc aggctgttcg ggccgcggcc gaccagggtc
     4921 aggcagagct tggccagcac ataggtcagc agggcgatca ccagcacgct gacccacagc
     4981 cagccggcct gcgtgacacc gagccaggcg ccgttcagca acagggacgc ggccgcccac
     5041 cagccggtct cgcgcgccgt gcggctgtgc gcgtgggcgg gcagccattg ccagatcagg
     5101 taggcggtca gccccacata gatcaccgac cagatgctga acgcctgcgt cgcgggggtg
     5161 agcagagtgg cgtcggcgct caacgcgccg ccggaactgc ggttcacttc g
//
LOCUS       DAOSUG010000178         5210 bp    DNA     linear   ENV 10-JUN-2024
DEFINITION  MAG TPA_asm: Micropruina sp. isolate AMR_MDS_2195
            NODE_7078_length_5210_cov_3.447915, whole genome shotgun sequence.
ACCESSION   DAOSUG010000178 DAOSUG010000000
VERSION     DAOSUG010000178.1
DBLINK      BioProject: PRJNA925477
            BioSample: SAMN32864262
            Sequence Read Archive: SRR4236673
KEYWORDS    WGS; ENV; Metagenome Assembled Genome; MAG; Third Party Data; TPA;
            TPA:assembly.
SOURCE      Micropruina sp. (wastewater metagenome)
  ORGANISM  Micropruina sp.
            Bacteria; Bacillati; Actinomycetota; Actinomycetes;
            Propionibacteriales; Nocardioidaceae; Micropruina.
REFERENCE   1  (bases 1 to 5210)
  AUTHORS   Abdulkadir,N., Saraiva,J.P., Zhang,J., Stolte,S., Gillor,O.,
            Harms,H. and Rocha,U.
  TITLE     Genome-centric analyses of 165 metagenomes show that mobile genetic
            elements are crucial for the transmission of antimicrobial
            resistance genes to pathogens in activated sludge and wastewater
  JOURNAL   Microbiol Spectr 12 (3), e0291823 (2024)
   PUBMED   38289113
REFERENCE   2  (bases 1 to 5210)
  AUTHORS   Abdulkadir,N.
  TITLE     Direct Submission
  JOURNAL   Submitted (24-MAR-2023) Environmental Microbiology, Helmholtz
            Center for Environmental Research -UFZ, Permoserstr 15, Leipzig
            04318, Germany
COMMENT     The annotation was added by the NCBI Prokaryotic Genome Annotation
            Pipeline (PGAP). Information about PGAP can be found here:
            https://www.ncbi.nlm.nih.gov/genome/annotation_prok/
            
            ##Genome-Assembly-Data-START##
            Assembly Method        :: SPAdes v. 3.11.1
            Genome Representation  :: Full
            Expected Final Version :: Yes
            Genome Coverage        :: Not Applicable
            Sequencing Technology  :: Illumina MiSeq
            ##Genome-Assembly-Data-END##
            
            ##Genome-Annotation-Data-START##
            Annotation Provider               :: NCBI
            Annotation Date                   :: 05/04/2023 04:57:10
            Annotation Pipeline               :: NCBI Prokaryotic Genome
                                                 Annotation Pipeline (PGAP)
            Annotation Method                 :: Best-placed reference protein
                                                 set; GeneMarkS-2+
            Annotation Software revision      :: 6.5
            Features Annotated                :: Gene; CDS; rRNA; tRNA; ncRNA
            Genes (total)                     :: 2,776
            CDSs (total)                      :: 2,750
            Genes (coding)                    :: 2,714
            CDSs (with protein)               :: 2,714
            Genes (RNA)                       :: 26
            tRNAs                             :: 24
            ncRNAs                            :: 2
            Pseudo Genes (total)              :: 36
            CDSs (without protein)            :: 36
            Pseudo Genes (ambiguous residues) :: 0 of 36
            Pseudo Genes (frameshifted)       :: 2 of 36
            Pseudo Genes (incomplete)         :: 32 of 36
            Pseudo Genes (internal stop)      :: 2 of 36
            ##Genome-Annotation-Data-END##
FEATURES             Location/Qualifiers
     source          1..5210
                     /organism="Micropruina sp."
                     /mol_type="genomic DNA"
                     /submitter_seqid="NODE_7078_length_5210_cov_3.447915"
                     /isolate="AMR_MDS_2195"
                     /isolation_source="Activated sludge of municipal
                     wastewater treatment plants"
                     /db_xref="taxon:2737536"
                     /environmental_sample
                     /geo_loc_name="Canada"
                     /lat_lon="49.25 N 123.1 W"
                     /collection_date="2015-06-16"
                     /metagenome_source="wastewater metagenome"
                     /note="metagenomic"
     gene            <1..709
                     /locus_tag="PLF56_07035"
     CDS             <1..709
                     /locus_tag="PLF56_07035"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_002525624.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=2
                     /transl_table=11
                     /product="HD domain-containing protein"
                     /protein_id="HOQ53354.1"
                     /translation="GANVSLTDAIALGHDCGHGPGGHASEDAFDAFIPEGYDHGPWGA
                     DVVLTDLNLCRETLDGIRNHSWSRPAPGTIEGEIVSWADRIAYCAHDLEDAIVAGIVT
                     AADVPPTVVEICGATRREQLRTFVREVIATTARTGSIGMGETVAGALAELRAFNYARI
                     YTRPESLAQSAAVVAVLQALVGHYLDHPELLPGEVHDADPVRATVAYVGGMTDRFAFD
                     TAVHLLGWNPQRLPRQQ"
     gene            complement(777..1499)
                     /locus_tag="PLF56_07040"
     CDS             complement(777..1499)
                     /locus_tag="PLF56_07040"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_002543195.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_function: GO:0042626 - ATPase-coupled transmembrane
                     transporter activity [Evidence IEA];
                     GO_function: GO:0140359 - ABC-type transporter activity
                     [Evidence IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="ABC transporter permease"
                     /protein_id="HOQ53355.1"
                     /translation="MVLLVAMAVTASAVGRLRLERQQLLAAVRAILQLAIISTVIAWA
                     VAQLWSALLLVAAMFAVAVFTTVRRVGAPRCWPWTALAMAAGVVPVLAIIFVTGASPF
                     TPVAVLALAGIVVGNAMTGHTLAGRRCFSELRSNRPSYEAALSIGLERPQAIGLVIED
                     VVPEALIPNLDQVRTVGLVTLPGAFIGVLLGGGSPMQAGAAQVLVLLGIMAGQAITVT
                     VAAAFICAGRLLPRDLAAELRP"
     gene            1577..3439
                     /gene="dnaG"
                     /locus_tag="PLF56_07045"
     CDS             1577..3439
                     /gene="dnaG"
                     /locus_tag="PLF56_07045"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_013161103.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_function: GO:0003896 - DNA primase activity [Evidence
                     IEA];
                     GO_process: GO:0006269 - DNA replication, synthesis of RNA
                     primer [Evidence IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="DNA primase"
                     /protein_id="HOQ53356.1"
                     /translation="MPGLINEEDLQTVRERTRIEDVVGSYVTLRPGGGGALVGLCPFH
                     DEKTPSFRVTPSRGFYYCFGCNAGGDVIGFVQQINNSSFVEAVEFLADRVGVQLRFTE
                     GGGGARVEPGLRSRILEANRLAAEFYAGQLSSPEAMIGRQFLAERGFDREAAEHFGVG
                     FAPQGGRELAKHLRAHKFTDDELIKASLIRDGGWDYFQGRLLWPIRDAGRSVLGFGAR
                     RLSDDDRMPAKYINTRETPVYKKSNVLYGLDLARQHIAKKNQAVIVEGYTDVMAAHLA
                     GVDTAVASCGTAFGDDHARLLSRLIGSSDALKGEVVFTFDGDAAGQAAALKVFSGDQH
                     FISQTYVAIEPSGLDPCDLRIQHGDAAVRELVARREPLYRYVMANTLAGFDLDRVDGR
                     LGALRAAAPLVGSVRDSSLVNGYIHELAKMLGTDVDEVRREVARAGRRKVPTQERPRN
                     EERVDAPEQPALGLPMPNPRDRSLEVERGTLRLIVRAPEVFTGGWNDVQPDDFTHPAY
                     RAIFEAVAAAGQPGADWPQPVVQASADSTAVHVIAALATEPLLRPANPTYAAEYVAKL
                     RLLSVSRAIAALKSKLQRTNPIDDQVNYNRMFAVLLDLEKQRRELAEAAAGPAL"
     gene            3532..4335
                     /locus_tag="PLF56_07050"
     CDS             3532..4335
                     /locus_tag="PLF56_07050"
                     /inference="COORDINATES: protein motif:HMM:TIGR02937.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_function: GO:0003677 - DNA binding [Evidence IEA];
                     GO_function: GO:0003700 - DNA-binding transcription factor
                     activity [Evidence IEA];
                     GO_function: GO:0016987 - sigma factor activity [Evidence
                     IEA];
                     GO_process: GO:0006352 - DNA-templated transcription
                     initiation [Evidence IEA];
                     GO_process: GO:0006355 - regulation of DNA-templated
                     transcription [Evidence IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="sigma-70 family RNA polymerase sigma factor"
                     /protein_id="HOQ53357.1"
                     /translation="MTDTLLEAADEIRLAKTIEAGLLAGELLARADFSFAPAAELLQV
                     EQLGRDAWQQFLLANVRLVQSFAYQASRRCDAGLDELFQEGFLGLAEAVQRWDHAAGY
                     RFSTYAVQWIRRRVLNAALSLDLAGPGSARTAQRARSVRQLAEELTGQLHRVATDVEV
                     AELLGRSSTWVARMRHLQPCAPLEPDLIAVELPMQESPRRLDLQQVLAALPWPEREVV
                     RRRFGLDGDEPLNQRATAEALGRSLSTVRRCEARALRRLRVWLSDDLAA"
     gene            4379..4882
                     /locus_tag="PLF56_07055"
     CDS             4379..4882
                     /locus_tag="PLF56_07055"
                     /inference="COORDINATES: ab initio
                     prediction:GeneMarkS-2+"
                     /note="Derived by automated computational analysis using
                     gene prediction method: GeneMarkS-2+."
                     /codon_start=1
                     /transl_table=11
                     /product="hypothetical protein"
                     /protein_id="HOQ53358.1"
                     /translation="MGLLDRAGLPSAEFRSLGEAVGGRVRVLAWARGDHGPVVGLSQA
                     LALRTVDGWRFVPWHTIDSGGWNAETARLRWRLVDGASDEITLDQPGSLPELFRERVD
                     ASIAVQERFELPRGRAAIIVARRRLDDDHAPLIWSSTQVGGTFDVAQSDQAEAELARL
                     KAEYDIA"
     gene            4921..4996
                     /locus_tag="PLF56_07060"
     tRNA            4921..4996
                     /locus_tag="PLF56_07060"
                     /product="tRNA-Asn"
                     /inference="COORDINATES: profile:tRNAscan-SE:2.0.12"
                     /note="Derived by automated computational analysis using
                     gene prediction method: tRNAscan-SE."
                     /anticodon=(pos:4954..4956,aa:Asn,seq:gtt)
BASE COUNT          798 a         1801 c         1847 g          764 t
ORIGIN      
        1 cggcgcgaac gtcagcctca ccgacgccat cgccctgggt cacgactgcg gtcacgggcc
       61 cggcggacac gcgtccgagg acgccttcga cgccttcatc cccgagggct acgaccacgg
      121 tccgtggggc gccgacgtcg tcctgaccga cctgaacctg tgccgcgaga ccctcgacgg
      181 gatccgcaac cactcgtgga gccgtccggc gccggggacg atcgagggcg agatcgtcag
      241 ctgggccgac cggatcgcct actgcgccca cgacctcgag gacgcgatcg tggccggcat
      301 cgtgaccgct gcggacgtgc cgcccacggt ggtcgaaatc tgcggtgcca cgcgtcgcga
      361 gcagctgcgc accttcgtcc gtgaggtgat cgcgaccacg gcccgcaccg gctcgatcgg
      421 catgggcgaa acggtggcgg gcgccctggc cgagttgcgc gccttcaact acgcacgcat
      481 ctacacccgg cccgagtcgc tggcccaatc agccgcggtg gtggcggtgc tgcaggcgct
      541 ggtgggtcac tacctggacc accccgagct gctgcccggt gaggtccacg acgccgaccc
      601 ggtacgcgcc acggtcgcct atgtgggcgg gatgaccgac cgcttcgcct tcgacaccgc
      661 ggtgcacctg ttgggctgga acccgcagcg gctgccgcgc cagcagtgag tatgacggcc
      721 gggcccccgg ctgcccagcg cggccggcct cctacgggtc ggacgcggtg gcgcactcac
      781 ggacgcagtt cggcggccag atctcgcggc agcagacgac cggcacagat gaaagcggcg
      841 gccacggtca cggtgatcgc ttggccggcc atgatgccga gcagcacgag tacctgcgcg
      901 gcacccgcct gcatcggtga tccgcccccg agcagcacgc cgatgaatgc gcccggcaga
      961 gtcaccaggc caaccgttcg gacctgatcc aggttcggaa tcaacgcctc gggcaccacg
     1021 tcctcgataa ccaggccgat cgcctgcggc cgctccagcc cgatcgacag cgccgcctca
     1081 tagctgggtc ggttgctgcg cagttcgctg aaacagcgtc gtccggccag cgtgtgcccg
     1141 gtcatcgcgt tgccgacgac gatgccggcc aacgccagca ccgcaacggg agtgaacggt
     1201 gatgccccgg tgacgaagat gatcgccagt acgggcacga cgccggcggc catcgcgagc
     1261 gccgtccaag gccaacagcg tggggcacca acccgccgca cagtggtgaa caccgcaacc
     1321 gcgaacatcg ctgcgaccag cagcaaggcc gaccacagct gcgcgaccgc ccaggcgatc
     1381 accgtcgaga tgatcgcgag ctgcaggatc gcccgcaccg cggccaggag ttgctggcgc
     1441 tccagccgca gccggccgac cgccgaggcg gtgacagcca ttgcgaccag taacaccagt
     1501 gcggccgcaa gaccccagcc cacggttacc gtcaccggtt cattatggcc attcgggctt
     1561 gcgtacactg ccggatgtgc ccgggctgat caatgaagaa gatctgcaga ccgtccgcga
     1621 gcgcacccgc atcgaggacg tggtcggctc ctacgtcacg ctgcggcccg gcggcggggg
     1681 agcgttggtg gggctgtgcc cctttcacga cgagaagacg ccctcgttcc gggtgacgcc
     1741 gtcgcgcggc ttctactact gcttcggctg caacgccggc ggcgacgtga tcggcttcgt
     1801 ccagcagatc aacaactcca gcttcgtcga ggcggtcgag tttctggccg accgggtggg
     1861 cgtgcaactg cgcttcaccg agggcggcgg gggtgcccgg gtcgagccgg gcctgcgctc
     1921 gcgcattctg gaggccaacc ggctggccgc cgagttctac gccgggcagt tgagcagccc
     1981 ggaggcgatg atcgggcggc agtttctggc cgagcgcggc ttcgaccgtg aggcggccga
     2041 acatttcggg gtgggcttcg cgccccaggg tggacgcgaa ctggccaagc acctgcgcgc
     2101 ccacaagttc accgacgacg aactgatcaa ggccagcctg atccgtgacg gcggctggga
     2161 ctactttcag ggccgcctgc tgtggccgat ccgcgatgcc ggacgctccg tgctgggctt
     2221 cggcgctcgg cggctctccg acgacgaccg catgccggcc aagtacatca acacccgcga
     2281 gacgccggtc tacaagaagt cgaacgtgct gtacgggctc gacctggccc ggcagcacat
     2341 cgccaagaag aaccaggcgg tgatcgtcga gggctacacc gacgtgatgg cggcgcacct
     2401 ggccggggtc gacaccgcgg tggcgtcctg cggcacggcc ttcggcgacg accacgcccg
     2461 gctgctgtcg cggctgatcg gcagttccga tgcgctcaag ggcgaggtgg tgttcacctt
     2521 cgacggcgac gccgcgggcc aggcggccgc gctgaaggtg ttctccggtg accagcactt
     2581 catctcgcag acctacgtgg cgatcgagcc gtccgggctc gacccctgcg acctgcgcat
     2641 tcagcacggg gacgccgcgg tgcgcgagct ggtcgcccgg cgcgagccgc tgtaccggta
     2701 cgtgatggcc aacacgctgg ccggtttcga cctcgaccgg gtggacggac ggctcggcgc
     2761 gctgcgcgcg gcggctccgc tggtgggcag cgtgcgcgat tcgtccctgg tcaacggcta
     2821 catccacgag ctggccaaga tgctcggcac cgacgtggac gaggtgcgcc gcgaggtagc
     2881 gcgggccggg cggcgcaagg tgccgacgca ggagcgtcca cgcaatgagg agagggtgga
     2941 cgcgcccgag cagccggctt tgggcctgcc gatgccgaac ccgcgggacc gctcgctgga
     3001 ggtcgagcgg ggcacgctgc ggctgatcgt gcgggcgccc gaggtgttca ccggcggctg
     3061 gaacgacgtg cagcccgacg acttcaccca tccggcgtac cgggcgatct tcgaagccgt
     3121 cgccgcagcc ggccagccgg gcgcggactg gccgcagccg gtggtgcagg catcagccga
     3181 ctcgacggcg gtgcacgtga tcgcggcctt ggcgaccgag ccgttgctgc gtccggccaa
     3241 cccgacgtac gcggcggaat atgtggccaa gctgcgactg ctcagcgtgt cgcgcgcgat
     3301 cgccgccctg aagtcgaagc tgcagcgcac caacccgatc gacgaccagg tcaactacaa
     3361 ccgcatgttc gccgtgctgc tcgacctgga gaagcagcgg cgcgagctcg cagaagcggc
     3421 ggccggcccg gcgctgtgag taccggcccg agatggtggt ggcaatctgc tgcctccttt
     3481 ggcattcgcg ggcaacaacc gtgacaacac cggggcgcca ttgtcgtgcc catgaccgac
     3541 accctgctcg aggccgccga cgagatccgg ctggccaaaa cgattgaagc cggcctgctg
     3601 gccggcgaac tgctcgcccg cgccgacttc tcgttcgcgc cggctgccga gttgctgcag
     3661 gtggagcaac tgggccgcga cgcctggcag cagtttctgt tggcgaacgt ccggctggtg
     3721 cagtcgttcg cctatcaggc atcgcgacgc tgcgacgccg gcctggacga gctgttccag
     3781 gagggctttc tcgggctcgc cgaggcggtg cagcggtggg atcacgccgc cggctaccgg
     3841 ttcagcacct acgccgtgca gtggatccgg cgccgcgtcc tgaatgccgc cctgtcgctc
     3901 gacctggccg gccccggctc ggcgcgcacg gcgcaacggg cgcgcagcgt ccggcagttg
     3961 gccgaagagc tgaccgggca gctgcaccgg gtggcgaccg acgtcgaggt ggccgagttg
     4021 ctcggccggt cgtccacctg ggtggcgcgc atgcggcacc tgcagccgtg cgccccgctc
     4081 gagcccgacc tgatcgccgt cgagctgccg atgcaggagt caccccggcg cctcgacctg
     4141 cagcaggtgt tggccgcctt gccctggccc gagcgcgagg tggtacgacg ccgcttcggc
     4201 ctggacggcg acgagcccct caaccagcgc gccaccgccg aggcgctcgg acgctccctc
     4261 agcaccgtgc ggcgctgcga ggcgcgcgcg cttcggcggc tgcgcgtgtg gctgagcgac
     4321 gacctggcgg catagctgcc gtcagcgggc ggggcggcgc ggctggcagt atggccgcat
     4381 gggccttctc gatcgcgccg ggctgccctc ggccgagttc cgcagcctcg gtgaggcggt
     4441 gggcggccgc gtgcgggtgc tcgcctgggc acgcggagac cacggcccgg tggtcggact
     4501 gtcgcaggcc ctggcgctgc gtaccgtgga cggctggcgg ttcgtgccgt ggcacaccat
     4561 cgacagcggg ggctggaacg ccgagacggc acgattgcgc tggcggctgg tcgacggggc
     4621 atccgatgag atcaccctcg accaaccggg ttcgctgcct gagctgtttc gcgagcgggt
     4681 ggacgccagc atcgcggtgc aggagcgatt cgagctgcca cgcggccggg ccgcgatcat
     4741 cgtggcgcga cgccggctgg acgatgacca tgccccgctg atctggtcgt cgacccaggt
     4801 ggggggcacg ttcgatgtcg cgcagagcga ccaggccgag gcggagcttg cccggctcaa
     4861 ggctgaatac gacatcgcgt gagaggtctg gtaacgtgtt ttctcgcgcc gcggcgctga
     4921 tcccctgtag ctcaattggc agagcgtcgg actgttaatc cgtaggttcc tggttcgagt
     4981 ccaggcgggg gagccacctt caaaaggcgt tctgacgagg taaaacacca gtcaggacgc
     5041 cttctgcttt tcggtcgcgg gcaacatgcg ggcaacagtg gcgggccgtc ctcccaaacg
     5101 aagggagcac cccaatgtcc accacctaca cccacgatca cgtgttcgcg gccggggagg
     5161 tcggctgtgt cgtttgccgg tcagcgcatc gtgaggattg cgactgcgag
//
LOCUS       DAOSUG010000179         5206 bp    DNA     linear   ENV 10-JUN-2024
DEFINITION  MAG TPA_asm: Micropruina sp. isolate AMR_MDS_2195
            NODE_7086_length_5206_cov_4.128907, whole genome shotgun sequence.
ACCESSION   DAOSUG010000179 DAOSUG010000000
VERSION     DAOSUG010000179.1
DBLINK      BioProject: PRJNA925477
            BioSample: SAMN32864262
            Sequence Read Archive: SRR4236673
KEYWORDS    WGS; ENV; Metagenome Assembled Genome; MAG; Third Party Data; TPA;
            TPA:assembly.
SOURCE      Micropruina sp. (wastewater metagenome)
  ORGANISM  Micropruina sp.
            Bacteria; Bacillati; Actinomycetota; Actinomycetes;
            Propionibacteriales; Nocardioidaceae; Micropruina.
REFERENCE   1  (bases 1 to 5206)
  AUTHORS   Abdulkadir,N., Saraiva,J.P., Zhang,J., Stolte,S., Gillor,O.,
            Harms,H. and Rocha,U.
  TITLE     Genome-centric analyses of 165 metagenomes show that mobile genetic
            elements are crucial for the transmission of antimicrobial
            resistance genes to pathogens in activated sludge and wastewater
  JOURNAL   Microbiol Spectr 12 (3), e0291823 (2024)
   PUBMED   38289113
REFERENCE   2  (bases 1 to 5206)
  AUTHORS   Abdulkadir,N.
  TITLE     Direct Submission
  JOURNAL   Submitted (24-MAR-2023) Environmental Microbiology, Helmholtz
            Center for Environmental Research -UFZ, Permoserstr 15, Leipzig
            04318, Germany
COMMENT     The annotation was added by the NCBI Prokaryotic Genome Annotation
            Pipeline (PGAP). Information about PGAP can be found here:
            https://www.ncbi.nlm.nih.gov/genome/annotation_prok/
            
            ##Genome-Assembly-Data-START##
            Assembly Method        :: SPAdes v. 3.11.1
            Genome Representation  :: Full
            Expected Final Version :: Yes
            Genome Coverage        :: Not Applicable
            Sequencing Technology  :: Illumina MiSeq
            ##Genome-Assembly-Data-END##
            
            ##Genome-Annotation-Data-START##
            Annotation Provider               :: NCBI
            Annotation Date                   :: 05/04/2023 04:57:10
            Annotation Pipeline               :: NCBI Prokaryotic Genome
                                                 Annotation Pipeline (PGAP)
            Annotation Method                 :: Best-placed reference protein
                                                 set; GeneMarkS-2+
            Annotation Software revision      :: 6.5
            Features Annotated                :: Gene; CDS; rRNA; tRNA; ncRNA
            Genes (total)                     :: 2,776
            CDSs (total)                      :: 2,750
            Genes (coding)                    :: 2,714
            CDSs (with protein)               :: 2,714
            Genes (RNA)                       :: 26
            tRNAs                             :: 24
            ncRNAs                            :: 2
            Pseudo Genes (total)              :: 36
            CDSs (without protein)            :: 36
            Pseudo Genes (ambiguous residues) :: 0 of 36
            Pseudo Genes (frameshifted)       :: 2 of 36
            Pseudo Genes (incomplete)         :: 32 of 36
            Pseudo Genes (internal stop)      :: 2 of 36
            ##Genome-Annotation-Data-END##
FEATURES             Location/Qualifiers
     source          1..5206
                     /organism="Micropruina sp."
                     /mol_type="genomic DNA"
                     /submitter_seqid="NODE_7086_length_5206_cov_4.128907"
                     /isolate="AMR_MDS_2195"
                     /isolation_source="Activated sludge of municipal
                     wastewater treatment plants"
                     /db_xref="taxon:2737536"
                     /environmental_sample
                     /geo_loc_name="Canada"
                     /lat_lon="49.25 N 123.1 W"
                     /collection_date="2015-06-16"
                     /metagenome_source="wastewater metagenome"
                     /note="metagenomic"
     gene            <1..328
                     /locus_tag="PLF56_07065"
     CDS             <1..328
                     /locus_tag="PLF56_07065"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_011756693.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=2
                     /transl_table=11
                     /product="hypothetical protein"
                     /protein_id="HOQ53359.1"
                     /translation="AKSLAAVAKEMGITHHPVQDLLVVGTPEGERLVPFVMALVPVVD
                     VAGGFVEVANLPGLIEPLNADDAAGAEGAAGAEGADDAAGADDADDADDTDDTDDTDD
                     AAGAAN"
     gene            328..1047
                     /gene="trmD"
                     /locus_tag="PLF56_07070"
     CDS             328..1047
                     /gene="trmD"
                     /locus_tag="PLF56_07070"
                     /EC_number="2.1.1.228"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_013862542.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_function: GO:0052906 - tRNA
                     (guanine(37)-N(1))-methyltransferase activity [Evidence
                     IEA];
                     GO_process: GO:0008033 - tRNA processing [Evidence IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="tRNA (guanosine(37)-N1)-methyltransferase TrmD"
                     /protein_id="HOQ53360.1"
                     /translation="MRLDIVTIFPGYFAPLQLSLVGKAIQRELVTVGVHDLRRWTHDR
                     HRTVDDTPYGGGAGMVMKPEPWGEALDELLLDPTPTDAEPLLIVPTPSGERFTQEYAA
                     EWAAEKRLLFACGRYEGIDARVVEHAAERMRVREVSLGDYVLNGGEVAVLAIIEAVVR
                     LLPGVLGNPASLVEESHAPGHDHLLEYPVYTKPETWRGLSVPDVLLSGHHGEVAKWRR
                     AQAEARTRARRPDLLAPPTPA"
     gene            1156..3345
                     /locus_tag="PLF56_07075"
     CDS             1156..3345
                     /locus_tag="PLF56_07075"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_009071801.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="amino acid transporter"
                     /protein_id="HOQ53361.1"
                     /translation="MTRRHPRDTALRRWFLQTQGQPEPEGFYEDEKEVVAHHHTQPWW
                     KVMCLTGVDYFSTLGYQPGIAALAAGALSPIATLVLVAVTLFGALPMYRRVAEESPHG
                     DGSLSMLERLLNYWPSKLLVLSLLGFVATGFVITITLSGADAAAHLVENPLLAQAVHG
                     YNVPITLGLIALLGVVFLIGFSEAIGVAVVLVIAYLGLSAVVIGNGLVEVVHHTDRLA
                     NWWTGLGNTFGSPVLMVAAAMLVFPALALGLSGFETGVVVMPLVKGDPTDTPQNPAGR
                     IRNAKKLLTGAAAIMSVMLIGSATVTTLLIPAGEFWPATTIARTVNSSDLDAGLVTMD
                     MPMDAQERPQIVTTVPLPAGRIGRYTVDVEAGGETVTMTVTETDQGGATLVTMSKPAG
                     GANGRALAYLAHERLGEGFGTVYDASTILILWFAGASAMAGLLNIVPRYLPRYGMAPE
                     WARSTRPLVVVFTLVAAVVTIAFQASVDRQAGAYATGVLALMTSAAFAVFLAELRRRH
                     RWAAVGFAVVCAIFVYTTVITIAERPEGLLIAAIFIATILFFSAMSRVVRSTELRVNH
                     VILEPQAEAIFARWAAAHPGPLRFISNKLDAGDRAEYDAKELDVRAATHLGKKKSAIF
                     LEVEVADASEFAADVRVGGELVGRHAVLRARGVAVPNTLAAIMLHVARMTEQPPHIYF
                     AWSERGPAENVMLFLMGGGGDIAPLTHEILRVAEPDESKRPQVHVGG"
     gene            3697..4521
                     /locus_tag="PLF56_07080"
     CDS             3697..4521
                     /locus_tag="PLF56_07080"
                     /EC_number="3.-.-.-"
                     /inference="COORDINATES: protein motif:HMM:NF013672.2"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_function: GO:0016787 - hydrolase activity [Evidence
                     IEA];
                     GO_function: GO:0046872 - metal ion binding [Evidence
                     IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="polysaccharide deacetylase family protein"
                     /protein_id="HOQ53362.1"
                     /translation="MNRTAPGLLTAFACALAMLAGCSQTQPAAAPSGTAEGPTAAATS
                     SATGAPSTTATAPAGSASPSPSEPAKVDCTKLKCVALTFDDGPGPKTPTLLATLVKEK
                     VPATFFMVGQMVKQHPDTSRQIAQTPGMTVGNHTMTHPNLRLIGIAKATREMTDNTKL
                     IAKVTGVKVRFMRPPYGSHNSATDAVAKKLGQAVVNWSAGALDWQYNTPAKIVDVTLP
                     QIAPGAIVLAHDIHPWTIEAVPTLIKKLRAKGYTLVSLDDILRNPRAGNTYSRGLH"
     gene            4607..>5206
                     /locus_tag="PLF56_07085"
     CDS             4607..>5206
                     /locus_tag="PLF56_07085"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_014846370.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="succinate dehydrogenase cytochrome b subunit"
                     /protein_id="HOQ53363.1"
                     /translation="MATTTLSPRARAVRSSVAMKALMAVTGLIMIGFLLMHMYGNLKV
                     FMGAEAFDHYAHWLKGDILYPLIPSGWFIWIFRAFMLAAIVLHAGSAWHLARKARIAA
                     GPKYVHKKTLQETYAARTMRWGGVILATFLVFHLLQFTAQVIKTGWNSGAGPYDMFVV
                     SFQQWWLVLAYALWMVTVCMHIRHGFWSAFATLGANTSAT"
BASE COUNT          797 a         1816 c         1763 g          830 t
ORIGIN      
        1 ggcgaaatcc ttggcggcgg tcgccaaaga gatgggcatc acccaccacc ccgtgcagga
       61 tctgctggtg gtcggcacgc ctgagggtga gcggctggtg cccttcgtga tggccctggt
      121 gccggtggtc gacgtggcgg gcggcttcgt cgaggtggcc aacctgcccg ggctgatcga
      181 gcctctgaac gcggacgacg cggcgggagc ggaaggcgcg gcgggagcgg aaggcgcgga
      241 cgacgcggcg ggagcggacg acgcggacga cgcggacgac acggacgaca cggacgacac
      301 ggacgacgcg gcgggagcgg ccaactgatg cgcctcgaca tcgtcacgat ctttcccggg
      361 tacttcgcgc cgctgcagtt gtcgctggtc ggcaaggcga ttcagcgcga actggtcact
      421 gtcggggtac atgacctgcg ccgctggacc cacgaccggc accgcaccgt cgacgacacc
      481 ccctacggcg gcggcgccgg catggtgatg aagcccgaac cctgggggga ggcactggac
      541 gagctgctgc tcgacccgac accgaccgac gccgagccgt tgctgatcgt gcccaccccg
      601 tccggcgaac gcttcaccca ggagtacgcc gccgaatggg cggccgagaa gcggctgctc
      661 ttcgcctgcg gacgctatga gggtatcgac gcccgggtgg tcgaacacgc cgccgagcgc
      721 atgcgggtgc gtgaggtcag cctgggtgac tacgtgctga acggcggcga ggtggcggtg
      781 ctggcgatca tcgaagcggt ggtgcggctg ctgccgggcg tgctcggcaa tccggcgtcg
      841 ctggtggagg agtcgcacgc cccgggtcac gaccacctgc tggagtaccc ggtctacacc
      901 aagccagaga cctggcgcgg gctcagcgtg ccggacgtgc tgttgtccgg tcatcacggc
      961 gaggtcgcga agtggcgccg ggcgcaggcc gaggcgcgga cgcgtgcgcg tcgtccggac
     1021 ctgctggccc ccccaacccc cgcctaactt tccagggccc gcgaagcttc gcaggcggac
     1081 gatggttagg cgggggttgg gcgatccccg caccgctctt ccccaggact ctgggccggt
     1141 ctagggttac cgaccgtgac ccggcgacac ccccgcgaca cggcccttcg ccgttggttc
     1201 cttcagacgc agggccagcc ggagcccgag ggcttctacg aggacgagaa agaggtcgtc
     1261 gcccaccacc acacacagcc ctggtggaag gtgatgtgcc tgaccggcgt cgactacttc
     1321 tcgaccctcg gctaccagcc cggcatcgcg gcgctggccg ccggtgccct gtcgccgatc
     1381 gccaccctgg tgctggtggc cgtcaccctg ttcggcgcgc tgccgatgta ccgacgggtc
     1441 gccgaagaga gcccgcacgg cgacggctcg ctgtcgatgc tcgagcgcct gctgaactac
     1501 tggccgtcca agctgttggt gctctcactg ctgggcttcg ttgccaccgg cttcgtgatc
     1561 accatcaccc tctcgggcgc ggacgccgcg gctcacctgg tcgagaaccc cctgctggcg
     1621 caggcggtgc acggctacaa cgtgccgatc acgctggggc tgatcgcact gctcggcgtg
     1681 gtctttctga tcggcttctc cgaggcgatc ggtgtggccg tggtgctggt gatcgcctac
     1741 ctgggtttga gcgcggtggt gatcggcaac ggtctggtcg aggtcgtcca tcacaccgac
     1801 cggctcgcca actggtggac ggggctcggc aacaccttcg gctccccggt gctgatggtc
     1861 gccgcggcca tgctggtgtt tccggcgctg gcgctgggcc tgtccggttt cgagaccggc
     1921 gtggtggtga tgccgctggt caagggcgac cccaccgaca ccccgcagaa tccggccgga
     1981 cgaatccgca acgccaagaa gctgctcacc ggcgcggccg ccatcatgag cgtgatgctg
     2041 atcggctcgg ccaccgtcac caccctgctg attcccgcgg gcgagttctg gccggcgacc
     2101 acgatcgccc gcacggtgaa cagctccgat ctggacgccg ggctggtcac catggacatg
     2161 ccgatggacg cccaggaacg accgcagatc gtcaccaccg tgccactgcc cgccggacgc
     2221 atcggcaggt acaccgtcga cgtcgaggcg ggcggcgaaa ccgtgacgat gaccgtcacc
     2281 gaaaccgatc agggcggcgc caccctggtc acgatgagca agcccgccgg cggcgccaac
     2341 ggacgtgccc tggcctacct ggcccacgaa cggttgggcg aggggttcgg caccgtctac
     2401 gacgcgtcca cgatcctgat cctttggttc gcgggagcat cggcgatggc cgggctgctc
     2461 aacatcgtgc cgcgctacct gccccgctac ggcatggcgc cggagtgggc ccgctcgacg
     2521 cgtccgctgg tggtggtgtt cacgctggtc gccgcggtgg tcaccatcgc ctttcaggcc
     2581 agcgtcgacc ggcaggcggg cgcctacgcc accggcgtgc tggccctgat gacctcggcc
     2641 gccttcgccg tgttcctggc cgagttgcgg cgccggcacc ggtgggcggc ggtcggcttc
     2701 gccgtggtct gcgcgatctt cgtctacacc acggtgatca ccatcgcgga gcgtcccgag
     2761 ggcctgttga tcgcggccat cttcatcgcg acgatcctgt tcttctcggc catgtcgcgc
     2821 gtggtgcgca gtaccgagtt gcgggtcaac cacgtgatcc tcgaaccgca ggccgaggcg
     2881 atcttcgcgc gctgggccgc cgcgcatccg ggcccgttgc ggttcatctc caacaagctg
     2941 gacgccggtg accgcgccga gtacgacgcc aaggagttgg acgtccgggc cgccacccac
     3001 ctgggcaaga agaagtccgc gatcttcctc gaggtcgagg tggcggacgc cagcgagttc
     3061 gccgccgacg tccgagtggg gggcgaactg gtcggacggc acgccgtgct gcgggccagg
     3121 ggagtggccg tgcccaacac gctggccgcg atcatgctgc acgtcgctcg catgaccgag
     3181 cagccaccgc acatctattt cgcctggtcc gagcggggtc cggccgagaa cgtgatgctg
     3241 ttcctgatgg gcggcggggg cgacatcgca ccgctgaccc acgagatcct gcgcgtcgcc
     3301 gagcccgatg agagcaagcg tccccaggtg cacgtgggcg gttgaggaac cgcgccgagc
     3361 cctccgccca ccccagcagc aggcagggtt cggatcggtg ggccgggctg agcccagctc
     3421 accgatctgc gtctactgcg cgccccgttc gggcgccccg gccgcgccgg cgcgtgccag
     3481 ccgccgcgcc acctgcaggc cgcgaggacg aacacggaca gcagcgcgag ccaccgccgc
     3541 ttcctccacc cctgttcggc gtggactccc tgttcggcgt ggactccctg gtcgacctgg
     3601 ttgcgatcgc tctccagacg cccacctgga cgttcctcgc cggccggtca gcgtttcggt
     3661 tcgtgtttcc tccgtgggag gaggttcact ggagcagtga accgcaccgc ccctggcctt
     3721 ctgaccgctt tcgcctgtgc acttgccatg ctcgccggct gctcgcagac ccaacctgcc
     3781 gcggcaccgt ccggtaccgc cgaggggccc acggccgcgg cgacgtccag cgccaccggc
     3841 gcaccctcga ccacggccac cgcgccggcc gggtccgcca gcccgagccc cagcgagccg
     3901 gccaaggtcg attgcaccaa gctcaagtgc gtggccctga ccttcgacga cgggccgggg
     3961 ccgaagaccc cgacgctgct ggccaccctg gtcaaagaga aggtgccggc cacgttcttc
     4021 atggtcgggc agatggtcaa gcagcacccc gatacgtcca ggcagatcgc gcagacgccc
     4081 ggcatgaccg tgggcaacca cacgatgacc catccgaacc tgaggctgat cggcatcgcg
     4141 aaggcgaccc gcgagatgac cgacaatacg aagctgatcg ccaaggtcac cggggtcaag
     4201 gtgcgcttca tgcgtccgcc ctatggctcg cacaacagtg ccaccgacgc ggtggcgaag
     4261 aagctcggcc aggccgtggt caactggagc gcgggggccc tcgactggca gtacaacacg
     4321 cccgcgaaga tcgtggacgt cacgctgccg cagatcgcgc ccggcgcgat cgtgctcgcc
     4381 cacgacattc atccgtggac gatcgaggcg gttccgaccc tgatcaagaa gctgcgcgcc
     4441 aagggctaca cgctggtcag cctggacgac attctgcgca atcctcgtgc cggaaatacg
     4501 tattcacgcg ggctgcactg accaatcgcc gagacctgcc gtctcagcca caagttcttc
     4561 tgcgtttggg ccgacgaggc cctggcaacc ggattaaggt ggctgcgtgg cgacgacaac
     4621 actttccccg cgtgcgcggg ccgtccgctc atccgtggcg atgaaggccc tgatggcggt
     4681 caccggtctg atcatgatcg gcttcctact gatgcacatg tacggcaacc tcaaggtctt
     4741 catgggtgcc gaggcattcg accattacgc ccactggctc aagggcgaca tcctctaccc
     4801 gctgatcccg tccggctggt tcatctggat cttccgggcc ttcatgctgg cggcgatcgt
     4861 gctgcacgcc ggctcggcgt ggcatctggc ccgcaaggcc aggatcgcgg ccggtcccaa
     4921 gtacgtccac aagaagacgc tgcaagagac ctacgccgcc cgcaccatgc gctggggcgg
     4981 cgtcatcctg gcgaccttcc tggtctttca cctgctgcag ttcaccgcgc aggtgatcaa
     5041 gaccggctgg aacagcggcg ccggccccta cgacatgttc gtggtgtcgt tccagcagtg
     5101 gtggctggtg ctggcctacg cgttgtggat ggtgaccgtc tgcatgcaca tccggcacgg
     5161 gttctggagc gccttcgcca ccctcggcgc gaacacctcg gcgacg
//
LOCUS       DAOSUG010000180         5205 bp    DNA     linear   ENV 10-JUN-2024
DEFINITION  MAG TPA_asm: Micropruina sp. isolate AMR_MDS_2195
            NODE_7089_length_5205_cov_5.033204, whole genome shotgun sequence.
ACCESSION   DAOSUG010000180 DAOSUG010000000
VERSION     DAOSUG010000180.1
DBLINK      BioProject: PRJNA925477
            BioSample: SAMN32864262
            Sequence Read Archive: SRR4236673
KEYWORDS    WGS; ENV; Metagenome Assembled Genome; MAG; Third Party Data; TPA;
            TPA:assembly.
SOURCE      Micropruina sp. (wastewater metagenome)
  ORGANISM  Micropruina sp.
            Bacteria; Bacillati; Actinomycetota; Actinomycetes;
            Propionibacteriales; Nocardioidaceae; Micropruina.
REFERENCE   1  (bases 1 to 5205)
  AUTHORS   Abdulkadir,N., Saraiva,J.P., Zhang,J., Stolte,S., Gillor,O.,
            Harms,H. and Rocha,U.
  TITLE     Genome-centric analyses of 165 metagenomes show that mobile genetic
            elements are crucial for the transmission of antimicrobial
            resistance genes to pathogens in activated sludge and wastewater
  JOURNAL   Microbiol Spectr 12 (3), e0291823 (2024)
   PUBMED   38289113
REFERENCE   2  (bases 1 to 5205)
  AUTHORS   Abdulkadir,N.
  TITLE     Direct Submission
  JOURNAL   Submitted (24-MAR-2023) Environmental Microbiology, Helmholtz
            Center for Environmental Research -UFZ, Permoserstr 15, Leipzig
            04318, Germany
COMMENT     The annotation was added by the NCBI Prokaryotic Genome Annotation
            Pipeline (PGAP). Information about PGAP can be found here:
            https://www.ncbi.nlm.nih.gov/genome/annotation_prok/
            
            ##Genome-Assembly-Data-START##
            Assembly Method        :: SPAdes v. 3.11.1
            Genome Representation  :: Full
            Expected Final Version :: Yes
            Genome Coverage        :: Not Applicable
            Sequencing Technology  :: Illumina MiSeq
            ##Genome-Assembly-Data-END##
            
            ##Genome-Annotation-Data-START##
            Annotation Provider               :: NCBI
            Annotation Date                   :: 05/04/2023 04:57:10
            Annotation Pipeline               :: NCBI Prokaryotic Genome
                                                 Annotation Pipeline (PGAP)
            Annotation Method                 :: Best-placed reference protein
                                                 set; GeneMarkS-2+
            Annotation Software revision      :: 6.5
            Features Annotated                :: Gene; CDS; rRNA; tRNA; ncRNA
            Genes (total)                     :: 2,776
            CDSs (total)                      :: 2,750
            Genes (coding)                    :: 2,714
            CDSs (with protein)               :: 2,714
            Genes (RNA)                       :: 26
            tRNAs                             :: 24
            ncRNAs                            :: 2
            Pseudo Genes (total)              :: 36
            CDSs (without protein)            :: 36
            Pseudo Genes (ambiguous residues) :: 0 of 36
            Pseudo Genes (frameshifted)       :: 2 of 36
            Pseudo Genes (incomplete)         :: 32 of 36
            Pseudo Genes (internal stop)      :: 2 of 36
            ##Genome-Annotation-Data-END##
FEATURES             Location/Qualifiers
     source          1..5205
                     /organism="Micropruina sp."
                     /mol_type="genomic DNA"
                     /submitter_seqid="NODE_7089_length_5205_cov_5.033204"
                     /isolate="AMR_MDS_2195"
                     /isolation_source="Activated sludge of municipal
                     wastewater treatment plants"
                     /db_xref="taxon:2737536"
                     /environmental_sample
                     /geo_loc_name="Canada"
                     /lat_lon="49.25 N 123.1 W"
                     /collection_date="2015-06-16"
                     /metagenome_source="wastewater metagenome"
                     /note="metagenomic"
     gene            <1..1609
                     /locus_tag="PLF56_07090"
     CDS             <1..1609
                     /locus_tag="PLF56_07090"
                     /EC_number="6.4.1.1"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_017934692.1"
                     /note="biotin-containing enzyme that catalyzes a two step
                     carboxylation of pyruvate to oxaloacetate; Derived by
                     automated computational analysis using gene prediction
                     method: Protein Homology."
                     /codon_start=2
                     /transl_table=11
                     /product="pyruvate carboxylase"
                     /protein_id="HOQ53364.1"
                     /translation="KFAEATPNIPQQILLRGRNTVGYTPYPLKVTNAFVHEAARTGCD
                     IFRIFDALNNIDQIRPAIEAVRETDHGVAEAAICYTGNLTSPSETLYTLDYYLHFAEQ
                     LVNAGAHILAIKDMAGLLRAPAAAKLVTALRERFDLPVHLHTHDTAGGQLATLLAAIN
                     AGVDAVDAASAAWAGTTSQVSMSALIAATDDTERATGLSLMAATDLEPYFEAVRLLYA
                     PFESGLPGPTGRVYLHEIPGGQLSNLRQQAIALGLGHRFEAIENMYAAANRILGNIVK
                     VTPSSKVVGDLALALVGANADPADFEANPGRYDIPASVIGFLGGELGDPPGGWPEPFR
                     TKALAGRTIKPVETELSAEDEAALAAEPRRTLNRLLFPGPTKDYEDAVDEFSELSVLA
                     TREFLHGLSVHQEHEVEIERGKKLLLGLEAIGQPDERGYRQVVCSINGQMRMVSVRDH
                     AAESHVVTRERADASKPGQVPAPFAGVVTLTVAEGDEVTAGQPVATIEAMKMEAAITA
                     PVAGTVERLAIGHAQAVEGGDLLLVIG"
     gene            1732..2760
                     /locus_tag="PLF56_07095"
     CDS             1732..2760
                     /locus_tag="PLF56_07095"
                     /inference="COORDINATES: protein motif:HMM:TIGR00350.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_process: GO:0070726 - cell wall assembly [Evidence
                     IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="LCP family protein"
                     /protein_id="HOQ53365.1"
                     /translation="MAGSNEGSSSETAPDSAFDDLLHEQASAAKPRRKGLRRGLIVLG
                     CLLVAMIVAAGSLLGWGIGQLNAVKREPGLLPSGRTTLDPSQLVNKPVNFLLLGSDSR
                     GEDRGRSDVMMLVHADPNRGALYLISLPRDLWVPIQGHGTNKINAAYSWGGAPLAVQT
                     VEQLLQIRIDNVALTDFEGFFNLIDDLGGVTVFNENPSANLGFTFPRGDIKLTGASAL
                     AFVRERYALPRGDFDRAERQRLVVKAIVDKLASKGTLTDPGKLVAVINRLSKSVTVDD
                     TLTNDRILTLVKDLQITSGEDIRQLQVPTAGFGTSKAGASYVKVDEAGMKRLSEALNN
                     GTMSTYQP"
     gene            complement(3023..3634)
                     /locus_tag="PLF56_07100"
     CDS             complement(3023..3634)
                     /locus_tag="PLF56_07100"
                     /inference="COORDINATES: protein motif:HMM:NF012655.2"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_function: GO:0003677 - DNA binding [Evidence IEA];
                     GO_function: GO:0003700 - DNA-binding transcription factor
                     activity [Evidence IEA];
                     GO_process: GO:0006355 - regulation of DNA-templated
                     transcription [Evidence IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="TetR/AcrR family transcriptional regulator"
                     /protein_id="HOQ53366.1"
                     /translation="MPRNGAPTRERILEAAERLVIDNGYAATSVDRVIAEAHSSKGSF
                     FHHFASKDDLARALVNRYVAADIAHLDAAVAEVTGATGDPRERVLQFLRVFEDSGDQI
                     MAAQSSCLYVSVLTERELVDRGTSAEIVRAIEAWREGLVALLRDAMPDEGPTDPAALA
                     DHVFVTFEGAFILCRATGDPGHMRSQLRVLRQLVDSLLPQPVD"
     gene            3723..4640
                     /locus_tag="PLF56_07105"
     CDS             3723..4640
                     /locus_tag="PLF56_07105"
                     /inference="COORDINATES: protein motif:HMM:NF014615.2"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="homocysteine S-methyltransferase family protein"
                     /protein_id="HOQ53367.1"
                     /translation="MNRTHLLDRPGGYVTDGGMETDLIFHHGVDLPQFAAFPLLDDPD
                     GRRLLRDYYDGYARIAAHAGAGLLLETPTWRANPDWGARVGYDAAALVRVNAAAVAFV
                     RSIADDWNDRVPEIRLVGMVGPRGDGYQAARHDPEQAAVYHQAQVRAFAEAGADLVNA
                     YTLTGPEEAIGVVLAARRAGIAAGVAFTVETDGRLPDGTALAEAIRVVDAACAPAYYG
                     INCAHPAHIAQGLDHAGPWRERIAMVRANASERSHAELDESVELDDGDPARWAVELRA
                     LHHLLPGLRVLGGCCGTDARHVAAAWHQW"
     gene            4872..>5205
                     /locus_tag="PLF56_07110"
     CDS             4872..>5205
                     /locus_tag="PLF56_07110"
                     /inference="COORDINATES: protein motif:HMM:NF017778.2"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="hypothetical protein"
                     /protein_id="HOQ53368.1"
                     /translation="MTITAPAAASPHLHAPDPALSRLLQRVVHDRDSWQPLIRFGVAE
                     RYWARIHVPEDVDVWLLTWTTAQGTELHDHGTSEAAFSVISGVLSETRVRDGELIVHE
                     RTPGAVVPV"
BASE COUNT          836 a         1751 c         1830 g          788 t
ORIGIN      
        1 gaagttcgcc gaggcgacgc cgaacattcc gcagcagata ctgctgcgcg gccgcaatac
       61 cgtcggctac accccctacc cgctcaaggt gaccaacgcc ttcgtccatg aggcggcgcg
      121 caccggctgc gacatcttcc ggatcttcga cgcgctgaac aacatcgacc agatccggcc
      181 ggcgatcgag gcggtgcgcg agaccgacca cggggtggcc gaggcggcga tctgctacac
      241 cggcaacctg accagcccgt ccgagacgct ctacaccctc gactactacc tgcacttcgc
      301 cgagcagctc gtgaacgccg gggcgcacat tctggcgatc aaggacatgg ccgggctgct
      361 gcgggcaccg gccgcggcga agctggtcac cgcgctgcgc gagcgcttcg acctgcccgt
      421 gcacctgcac acccacgaca ccgcgggcgg ccagctggcc accctgctgg cggcgatcaa
      481 tgccggcgtc gatgccgtgg acgcggcctc ggcggcctgg gcgggcacca cctcccaggt
      541 ctcgatgtcg gcgctgatcg ccgcaacgga cgacaccgag cgggccaccg ggctgtcact
      601 gatggccgcc accgacctgg agccctactt cgaggccgtc cggttgctgt acgcgccgtt
      661 cgagtcgggg ctgcccggcc cgaccggacg ggtctacctg cacgagattc cgggcggtca
      721 gctgagcaac ctgcggcagc aggcgatcgc gctggggctc gggcaccggt tcgaggcgat
      781 cgagaacatg tacgccgcgg cgaaccgcat tctcggcaac atcgtcaagg tcaccccatc
      841 gtccaaggtg gtgggcgatc tggcgctggc attggtcggg gccaacgccg accccgccga
      901 cttcgaggcc aacccgggcc gctacgacat tccggcgtcg gtgatcggct ttctgggcgg
      961 cgagctgggc gatccgcccg gcggctggcc tgaaccgttc cgcaccaagg cgctggccgg
     1021 acgcacgatc aagccggtcg agaccgagct gtcggccgag gacgaggccg ccctggccgc
     1081 cgaaccgcgg cgcacgctga accggctgct gttccccggc ccgaccaagg actacgagga
     1141 cgcggtggac gagttctccg agctctcggt gctcgccacc cgggagtttc tgcacgggct
     1201 gagcgtgcat caggagcacg aggtcgagat cgagcggggc aagaagctgc tgctgggtct
     1261 cgaggcgatc ggccagccgg acgagcgcgg ctaccgccag gtggtctgct cgatcaacgg
     1321 gcagatgcgg atggtctcgg tgcgcgacca cgcggcggag tcgcacgtgg tcacccgcga
     1381 gcgtgcggac gcgtccaagc ccggccaggt gccggcgccc ttcgccggcg tggtcacgct
     1441 cacggtggcc gaaggcgacg aggtgacggc cggccagccg gtggccacca tcgaggcgat
     1501 gaagatggag gccgccatca ccgcaccggt ggccggcacc gtcgagcggc tggcgatcgg
     1561 ccacgcgcag gcggtcgagg gcggtgacct gctgctggtg atcggctgag cgcccaaccc
     1621 ccgcctaacc atcgtgcgcc tgcgaagctt cgcaggcgca cgatggttag gcgggcgttc
     1681 gcagcggcca cgtaagctgg accggttcgc acgcgagagg ggggctcacg agtggccggc
     1741 agtaacgagg ggtcgtccag cgagacggcg ccggacagtg cattcgacga cctactgcac
     1801 gagcaggcct cggccgcgaa gcctcgtcgc aaggggttgc ggcgtgggct gatcgtgctg
     1861 gggtgcctgc tggtcgccat gatcgtggcg gcggggtcgt tgctgggctg gggtatcggg
     1921 cagctcaacg cggtgaagcg tgaacccggc ctgctgccgt ccggacggac cacgctcgac
     1981 ccgagccaac tggtgaacaa gccggtcaac tttctgctgc tcggctcgga ctcgcgcggc
     2041 gaggatcgcg gccgctccga cgtgatgatg ctggtgcacg ccgaccccaa ccgcggcgcg
     2101 ctgtacctga tttcgctgcc gcgcgacctg tgggtaccga ttcaggggca cggcaccaac
     2161 aagatcaacg ccgcctactc atggggcggt gcgccgctgg cggtgcagac cgtcgagcag
     2221 ttgctgcaga tccgcatcga caatgtggcg ctgaccgact tcgaggggtt cttcaacctg
     2281 atcgacgacc tgggtggcgt cacggtgttc aacgagaacc cgtcggccaa cctggggttc
     2341 acttttccgc gcggtgacat caagctgacc ggggcgtccg cgctggcctt cgtgcgcgag
     2401 cgctatgcgc tgccccgcgg tgatttcgac cgggccgagc ggcagcggct ggtggtgaag
     2461 gcgatcgtcg acaagctggc cagcaagggc acgctgaccg atccgggcaa actggtggcg
     2521 gtgatcaacc ggctctcgaa gtcggtgacc gtggacgata ccctcaccaa cgaccgcatt
     2581 ctgacgctgg tgaaggacct gcagatcacc tcgggcgaag acatccgaca actgcaggtt
     2641 ccgaccgccg gtttcggaac gtccaaggcg ggtgcgtcgt acgtgaaggt ggacgaggcg
     2701 ggcatgaagc ggctgtccga ggccctgaac aacggcacga tgagcaccta tcagccctag
     2761 tgaagggttt gttcaacgca cgatcggtcg gttgagcccg tcgaaaccgt tgcacgaggt
     2821 cttcaccagc ggcgtgtccg tcatcgcggg tttgcccctg gatctcggcg cgcgcagagg
     2881 tctcggcgtg tcccgggatg acgagggctc gacgtgtctg ggaagtgctg ataaacgcac
     2941 gatgcgatgg ttgagcctgt cgaagcccgc ctcgcacacg tctcgacagg ctcgtccagc
     3001 gagaggatcg gtggagccgg gctcaatcga caggttgggg cagcagcgag tccacgagtt
     3061 gccgcagcac ccgcagttga ctgcgcatgt gaccggggtc accggtggcc cggcacagaa
     3121 tgaaggcacc ctcgaaggtc acgaagacgt ggtcggccag cgccgccggg tcggtcggac
     3181 cctcgtcggg catggcgtcg cgcagtagcg cgaccaggcc ctcgcgccag gcctcgatgg
     3241 cccggacgat ctcggccgag gtgccgcggt cgaccagttc acgctcggtc agcaccgaca
     3301 cgtacaggca actgctctgg gccgccatga tctgatcgcc ggagtcctcg aagacgcgca
     3361 gaaactgcag cacccgctcg cgcggatcgc cggtcgcgcc ggtgacctcg gccacggcgg
     3421 cgtccagatg agcgatgtcg gccgcgacgt agcggttgac cagggcgcgc gcgaggtcgt
     3481 ccttgctcgc gaagtggtgg aagaacgagc ccttgctcga gtgggcctcg gcgatcaccc
     3541 ggtcaaccga cgtggcggcg tagccgttgt cgatcacgag gcgctcggcg gcttccagga
     3601 tgcgttcccg ggtcggggct ccattgcgtg gcacgaaccc cttgttacca gaccggacgg
     3661 tctgatacaa ctggaccact cagtctagac caagcggtct ggtacgacga aggaggggtc
     3721 gcatgaaccg cacgcacctg ctggatcgcc cgggcggcta tgtcaccgac ggcggcatgg
     3781 agaccgacct gatctttcac cacggcgtgg atctgccgca gttcgccgcc tttccgttgc
     3841 tcgacgatcc ggacggacga cggctgctgc gggactacta cgacggctac gcccggatcg
     3901 cggcgcacgc cggggccgga ctgctgctcg agactcctac ctggcgggcc aatcccgact
     3961 ggggtgcccg ggtcggctac gacgcggcgg cgctggtccg ggtgaacgcc gcggccgtcg
     4021 ccttcgtccg ctcgatcgcg gacgactgga acgaccgagt gcccgagatt cggttggtcg
     4081 gcatggtggg accccggggc gacgggtatc aggcggcgcg gcacgatccc gagcaggccg
     4141 cggtctatca ccaggcgcag gtgcgtgcct tcgccgaggc gggcgccgac ctggtcaacg
     4201 cctacaccct gaccgggccc gaggaggcga tcggagtggt cctggctgcc cgaagggccg
     4261 gaatcgcggc cggtgtggcg ttcaccgtcg aaaccgacgg ccggctaccc gacggcaccg
     4321 ccctcgccga ggccattcgc gtggtcgacg cggcgtgcgc gccggcgtac tacggcatca
     4381 actgcgccca tccggcgcac atcgcccagg ggctggacca cgccggtccc tggcgcgagc
     4441 ggatcgccat ggtgcgggcc aacgcgtccg agcgcagcca tgccgaactc gacgaaagcg
     4501 tcgaactgga cgacggcgac ccggcccgct gggccgtgga gcttcgggcg ctgcaccacc
     4561 tgctgccggg gctgcgggtg ctgggcgggt gttgcggcac cgacgctcgg catgtggcgg
     4621 ccgcttggca tcaatggtga taccaatgcc gccttggcgg acgatgtccg ttgccacccg
     4681 ccgcacgctc gggctagcgt cgggcaacga tccatccagg gcggccgagg ttgccgccgg
     4741 gaacgctgga ggtagtcgat gttctccgac cgaatcgggt gtggcccgtg gtgtcgtact
     4801 gattgctgtt gttgtcgctg accagtaccc accagccctc gccatcacct cgattcacct
     4861 ggagaaacct catgaccatc accgcgcccg cggccgcttc gccgcacctt cacgcacccg
     4921 acccggccct gtcccgactg ctgcaacggg tcgtccacga tcgcgacagc tggcaaccgc
     4981 tgatccggtt cggcgtcgcc gaacgctact gggcccgcat ccacgtgccg gaggacgtgg
     5041 acgtctggct gctcacctgg accactgcgc agggcacgga actgcatgac cacggcacgt
     5101 ccgaggcggc gttcagcgtg atcagcgggg tgctgagcga aacccgcgtg cgcgacggcg
     5161 aactgatcgt gcacgagcgc acgcccggtg cggtggtgcc ggtgc
//
LOCUS       DAOSUG010000181         5172 bp    DNA     linear   ENV 10-JUN-2024
DEFINITION  MAG TPA_asm: Micropruina sp. isolate AMR_MDS_2195
            NODE_7149_length_5172_cov_3.886261, whole genome shotgun sequence.
ACCESSION   DAOSUG010000181 DAOSUG010000000
VERSION     DAOSUG010000181.1
DBLINK      BioProject: PRJNA925477
            BioSample: SAMN32864262
            Sequence Read Archive: SRR4236673
KEYWORDS    WGS; ENV; Metagenome Assembled Genome; MAG; Third Party Data; TPA;
            TPA:assembly.
SOURCE      Micropruina sp. (wastewater metagenome)
  ORGANISM  Micropruina sp.
            Bacteria; Bacillati; Actinomycetota; Actinomycetes;
            Propionibacteriales; Nocardioidaceae; Micropruina.
REFERENCE   1  (bases 1 to 5172)
  AUTHORS   Abdulkadir,N., Saraiva,J.P., Zhang,J., Stolte,S., Gillor,O.,
            Harms,H. and Rocha,U.
  TITLE     Genome-centric analyses of 165 metagenomes show that mobile genetic
            elements are crucial for the transmission of antimicrobial
            resistance genes to pathogens in activated sludge and wastewater
  JOURNAL   Microbiol Spectr 12 (3), e0291823 (2024)
   PUBMED   38289113
REFERENCE   2  (bases 1 to 5172)
  AUTHORS   Abdulkadir,N.
  TITLE     Direct Submission
  JOURNAL   Submitted (24-MAR-2023) Environmental Microbiology, Helmholtz
            Center for Environmental Research -UFZ, Permoserstr 15, Leipzig
            04318, Germany
COMMENT     The annotation was added by the NCBI Prokaryotic Genome Annotation
            Pipeline (PGAP). Information about PGAP can be found here:
            https://www.ncbi.nlm.nih.gov/genome/annotation_prok/
            
            ##Genome-Assembly-Data-START##
            Assembly Method        :: SPAdes v. 3.11.1
            Genome Representation  :: Full
            Expected Final Version :: Yes
            Genome Coverage        :: Not Applicable
            Sequencing Technology  :: Illumina MiSeq
            ##Genome-Assembly-Data-END##
            
            ##Genome-Annotation-Data-START##
            Annotation Provider               :: NCBI
            Annotation Date                   :: 05/04/2023 04:57:10
            Annotation Pipeline               :: NCBI Prokaryotic Genome
                                                 Annotation Pipeline (PGAP)
            Annotation Method                 :: Best-placed reference protein
                                                 set; GeneMarkS-2+
            Annotation Software revision      :: 6.5
            Features Annotated                :: Gene; CDS; rRNA; tRNA; ncRNA
            Genes (total)                     :: 2,776
            CDSs (total)                      :: 2,750
            Genes (coding)                    :: 2,714
            CDSs (with protein)               :: 2,714
            Genes (RNA)                       :: 26
            tRNAs                             :: 24
            ncRNAs                            :: 2
            Pseudo Genes (total)              :: 36
            CDSs (without protein)            :: 36
            Pseudo Genes (ambiguous residues) :: 0 of 36
            Pseudo Genes (frameshifted)       :: 2 of 36
            Pseudo Genes (incomplete)         :: 32 of 36
            Pseudo Genes (internal stop)      :: 2 of 36
            ##Genome-Annotation-Data-END##
FEATURES             Location/Qualifiers
     source          1..5172
                     /organism="Micropruina sp."
                     /mol_type="genomic DNA"
                     /submitter_seqid="NODE_7149_length_5172_cov_3.886261"
                     /isolate="AMR_MDS_2195"
                     /isolation_source="Activated sludge of municipal
                     wastewater treatment plants"
                     /db_xref="taxon:2737536"
                     /environmental_sample
                     /geo_loc_name="Canada"
                     /lat_lon="49.25 N 123.1 W"
                     /collection_date="2015-06-16"
                     /metagenome_source="wastewater metagenome"
                     /note="metagenomic"
     gene            complement(<1..491)
                     /locus_tag="PLF56_07115"
     CDS             complement(<1..491)
                     /locus_tag="PLF56_07115"
                     /inference="COORDINATES: protein motif:HMM:TIGR01643.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="RHS repeat protein"
                     /protein_id="HOQ53369.1"
                     /translation="MTPPHGAPASTTTTDIKGRTTKLTQHLASDSTTTYTYTPASYLA
                     SMTDPKGNKWSYTYDVQGNQLTATDPDKGTSTKTYNALDLPATVKDARNKGVSYTYDA
                     LDRVTATADLAGTTQLTSTAFDPNNGRAVSSTRYIKEPAGTETRAIQATIDKEGLAAP
                     QRKA"
     gene            681..1916
                     /locus_tag="PLF56_07120"
     CDS             681..1916
                     /locus_tag="PLF56_07120"
                     /inference="COORDINATES: protein motif:HMM:TIGR00710.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_function: GO:0022857 - transmembrane transporter
                     activity [Evidence IEA];
                     GO_process: GO:0055085 - transmembrane transport [Evidence
                     IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="multidrug effflux MFS transporter"
                     /protein_id="HOQ53370.1"
                     /translation="MTDERVGPAVATSLVLTLALQSAVPPFATDTYTPAFPHVVAELS
                     TTAGAVGLTLTAFFLGMALGQLVGGPLSDRHGRRRPMLIGGLLCTLGAVGCALAPSIG
                     FLIVFRVLQGFGGGVASVVARAVVVDVAHGDALAKVMSIMMALGGLAPMVAPVVGGAV
                     LTLGGSWRAVFWVLVALGVAMLATAYVFVPESLPPENRRGGELRELSAGLLKVVRLPV
                     FVGYTLTAALSGFTMMAYIANSSYVLQQQKGMAPMPFALFFAATAGTQVLLSLVNARL
                     VGRFRPRTLVGFGLAGSAAAVAILAIAVFALDTPLLLTCAGFMILMAMQAFIFGNASA
                     LAVAQAPEVAGAASGVLGVVQAIAMAVSAPLASLGGATTATPMIWVMLAGVAGAITAY
                     LIVSRSPGYPSRSSLGTPQ"
     gene            complement(2077..3429)
                     /gene="glmM"
                     /locus_tag="PLF56_07125"
     CDS             complement(2077..3429)
                     /gene="glmM"
                     /locus_tag="PLF56_07125"
                     /EC_number="5.4.2.10"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_020388066.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_function: GO:0008966 - phosphoglucosamine mutase
                     activity [Evidence IEA];
                     GO_process: GO:0009103 - lipopolysaccharide biosynthetic
                     process [Evidence IEA];
                     GO_process: GO:0009252 - peptidoglycan biosynthetic
                     process [Evidence IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="phosphoglucosamine mutase"
                     /protein_id="HOQ53371.1"
                     /translation="MARLFGTDGVRGRANAELTAELALDLAVAAAHVLGEAGVFEGRR
                     PFAVVGRDTRASGEFLEAAVVAGLASAGVDVVRLGVIPTPGVAYLTGALDADLGVMLS
                     ASHNPMPDNGIKFFARGGVKLDDVVEDAIEQRLGDEWTRPTGADVGRVRDDIGAVEGY
                     IAHLVASLGADSSLAPLKVVIDTANGAASLTALAAFDAQGAVVIPLHAWPDGLNINEN
                     CGSTHLESLQAAVLEHGADLGIGLDGDADRCLAVDATGRIVDGDQILAILALAMKENH
                     ALHSDTVVATVMSNLGFILAMRAHGIRVDQAKVGDRYVLESMNANGFTLGGEQSGHVI
                     MSEFATTGDGVLTALHLAARMARTGRTLADLASVMTQLPQVMINVPNVNKVRAGLDPV
                     LQQAVTAASRELGDTGRVLLRPSGTEPLVRVMVEAGTREQAQAIAERLVEVVKARLSL
                     "
     gene            complement(3437..3955)
                     /gene="rpsI"
                     /locus_tag="PLF56_07130"
     CDS             complement(3437..3955)
                     /gene="rpsI"
                     /locus_tag="PLF56_07130"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_016666057.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_component: GO:0005840 - ribosome [Evidence IEA];
                     GO_function: GO:0003735 - structural constituent of
                     ribosome [Evidence IEA];
                     GO_process: GO:0006412 - translation [Evidence IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="30S ribosomal protein S9"
                     /protein_id="HOQ53372.1"
                     /translation="MTETVETVETTEDLAPFTEGDREIAYRAEAVPSSAAPGVRPAVI
                     GAADATGRRKEAIARVRLTPGTGQWKVNGRTLEEYFPNKLHQQTVNEPFETAAVAGSY
                     DVIAKIVGGGVTGQAGALRLGIARALNAVDAEASRPALKKAGLLTRDARVKERKKAGL
                     KKARKAPQYSKR"
     gene            complement(3973..4416)
                     /gene="rplM"
                     /locus_tag="PLF56_07135"
     CDS             complement(3973..4416)
                     /gene="rplM"
                     /locus_tag="PLF56_07135"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_003866468.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_component: GO:0000315 - organellar large ribosomal
                     subunit [Evidence IEA];
                     GO_component: GO:0022625 - cytosolic large ribosomal
                     subunit [Evidence IEA];
                     GO_function: GO:0003735 - structural constituent of
                     ribosome [Evidence IEA];
                     GO_process: GO:0006412 - translation [Evidence IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="50S ribosomal protein L13"
                     /protein_id="HOQ53373.1"
                     /translation="MSTYSPKSGEVTRNWHVIDASDVVLGRLAVTAATLLRGKHKAQF
                     APHLDTGDFVIVVNASKIALTGSKRTNKMAYRHSGRPGGLKAVPYGELLATDPRNAVE
                     RAVWGMLPKNKLSRQLIKKLKVYSGPEHPHTAQQPQPYQITQIAQ"
     gene            4635..>5172
                     /locus_tag="PLF56_07140"
     CDS             4635..>5172
                     /locus_tag="PLF56_07140"
                     /EC_number="2.4.-.-"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_017934636.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="glycosyltransferase family 39 protein"
                     /protein_id="HOQ53374.1"
                     /translation="MTTSTIDSPANEARSPGPPAADTPAATTPERGPSTLVRRASLTL
                     TLLAVLALYTWNLAASGYANSFYSAAAQAGAESWKAFFYGSLDAGNSITVDKTPGALW
                     LMALSVRLFGLSSWSILMPQALLGVASAWILYGTVRRSLAGPLGKRLNDAVTSRDAAL
                     EPISATRAHWAGIVAALAF"
BASE COUNT          690 a         1739 c         1813 g          930 t
ORIGIN      
        1 gccttgcgct gcggcgcggc caatccttcc ttgtcgatgg tggcctggat ggcccgggtt
       61 tcggttccgg ctggttcctt gatgtagcgg gtgctgctga ctgcccgccc gttgttgggg
      121 tcgaaagcag tgctggtgag ttgcgtggtg ccggccaggt cagcggtggc ggtgaccctg
      181 tcgagagcgt cgtaggtgta gctgacgccc ttgttgcgag catccttgac cgtggcgggc
      241 aggtcgagcg cattgtaggt cttggttgat gtgcccttgt cagggtcggt cgcggtgagc
      301 tggttgcctt gcacgtcata ggtgtaggac cacttgttgc ccttcgggtc cgtcatggac
      361 gcgagatagc tggcgggtgt gtaggtgtag gtggtggtcg aatccgacgc cagatgctgg
      421 gtcagcttgg tggtgcggcc cttgatgtcg gtcgttgtgg tgctggcggg cgcaccgtgg
      481 ggtggggtca ccttgatcgt gttgccgcca tagacggagc tggtctgcca caggaagtcg
      541 ttgcccgagt acgtctgctg agcaagctga ggttcacccc aaacctatag acctgcgtcg
      601 agggccgggg gcacgctttt gacagccatt ggcgatattc ttgcgaaaat gcacacaagg
      661 acctgttcag gcctcacgtc atgacggatg aacgcgtggg cccggccgtc gcgacctctc
      721 tggtgctgac cctggctctg cagagcgccg taccgccgtt cgccaccgac acctacaccc
      781 cggcgtttcc ccacgtggtg gccgagttgt cgaccacggc cggcgccgtg ggtctcaccc
      841 tgacggcgtt ctttctgggc atggcgctgg ggcagttggt cggcggcccg ctctcggacc
      901 ggcacgggcg ccgccgtccg atgctgatcg gaggcctgct ctgcacgctg ggcgcggtcg
      961 ggtgcgcgct cgctccgtcc atcgggtttc tcatcgtctt tcgggtgctg cagggcttcg
     1021 gtggcggagt cgcgtccgtg gtcgcccgcg cagtggtggt cgacgtggcc cacggcgacg
     1081 ccctggccaa ggtgatgtcg atcatgatgg cgctgggcgg gctggccccc atggtggcgc
     1141 cggtggtggg cggtgccgtg ctgaccctgg gcggcagttg gcgcgcggtg ttctgggtgc
     1201 tggtcgccct cggcgtcgcg atgctggcga ccgcctacgt gttcgtgccc gaatcgctgc
     1261 cacccgagaa ccggcgcgga ggcgaactgc gcgaactcag cgcgggcctg ctgaaggtcg
     1321 tgcggctgcc ggtcttcgtc ggctacaccc tcaccgccgc cctgtcgggc ttcaccatga
     1381 tggcctacat cgcgaactcg tcctatgtgc tgcagcagca gaagggcatg gcgccgatgc
     1441 cgttcgctct gttcttcgct gcgaccgccg gcacgcaggt gctgctgtcg ctggtgaacg
     1501 cccggctggt cggacgtttc cgtccgcgca cgttggtcgg cttcgggctg gcgggatccg
     1561 ccgccgccgt cgcgattctc gcgatcgccg tgttcgccct cgatacgccg ctgctgctga
     1621 cctgtgcggg cttcatgatt ctgatggcga tgcaggcctt catcttcgga aacgccagcg
     1681 cactggcggt cgcgcaggcg ccggaggtgg ccggggcggc gtccggggtg ctcggcgtgg
     1741 tgcaggcgat cgccatggcg gtctcggcac ccctggccag cctcggtggc gcgaccacgg
     1801 cgacgccgat gatctgggtg atgctggccg gcgtcgccgg ggcgatcacg gcctacctga
     1861 tcgtgtcccg ttcgccgggc tacccgagca ggtcaagcct cggcacgccc cagtaggcgg
     1921 cgagctggtc acgccggtgc gccgcgtccc agccggtcag cggtgggatc aggcgtgcgg
     1981 cggccggtgc cgcgtccggt tcttcgatgg ccaggtgggt tgcgccgggt cagcacgtcg
     2041 agcaggcctg gacgccttcg ccggccgatt acggccctac agcgacaacc gcgccttgac
     2101 gacctcgacc agccgctcgg cgatcgcctg ggcctgctca cgggtgccgg cctcgaccat
     2161 cacccggacc agcggttcgg tgccggacgg gcgcagcagc acccgtccgg tgtcgcccaa
     2221 ctcacgcgat gccgcggtga ccgcctgctg cagcaccggg tcgagtccgg cgcgcacctt
     2281 attgacgttg ggcacgttga tcatcacctg cggcaactgg gtcatcaccg aggccaggtc
     2341 ggccagcgtt cgtccggtac gggccatgcg ggcggccaga tgcagcgccg tcagcacgcc
     2401 gtcgccggtg gtggcgaact cgctcatgat cacgtgcccg gactgctcac caccgagcgt
     2461 gaagccgttg gcgttcatgg attcgagcac gtagcggtcg ccgaccttgg cctggtcgac
     2521 ccggatgccg tgggcgcgca tcgccagaat gaagccgagg ttgctcatca cggtggcgac
     2581 caccgtgtcg ctgtgcagcg cgtggttctc tttcatcgcc agcgccagaa tcgcgaggat
     2641 ctgatcgccg tcaacgatcc ggcccgtcgc atcgacggcg aggcaacggt cggcgtcgcc
     2701 gtccagaccg atgcccaggt cggcgccgtg ctcgagtacc gcggcctgca gtgactccag
     2761 gtgtgtcgag ccgcagttct cattgatgtt gaggccgtcc ggccaggcat gcagcggaat
     2821 caccacagca ccctgcgcgt cgaaggcggc cagcgcggtg agcgaggccg caccgttggc
     2881 ggtgtcgatc accaccttga gcggagccag cgacgaatcg gcacccaggc tcgccaccag
     2941 gtgggcgatg tagccctcga ccgccccgat atcgtcacgg acgcgtccga catcggcgcc
     3001 ggtgggccgc gtccattcgt cgccgaggcg ttgctcgatg gcgtcctcga cgacgtcgtc
     3061 gagcttgacc ccgccgcggg cgaagaattt gatgccgttg tcgggcatcg gattgtggct
     3121 cgccgagagc atgacgccca ggtcggcgtc cagagcgccg gtgaggtagg ccacgccggg
     3181 cgtcggaatg acgccgagcc gcaccacgtc gacgccggcc gatgccaacc cggccaccac
     3241 ggccgcttcg agaaactcac cggaggcccg ggtgtcccgt ccgacgaccg cgaacggccg
     3301 acgaccctcg aacacgcccg cctcgcccag cacgtgggcg gcggcgacgg cgagatcgag
     3361 ggccagttcg gcggtcagct cggcgttggc tcggcctcgg acaccatcgg tgccgaacag
     3421 gcgtgccata cgccgatcag cgcttgctgt actgcggcgc cttgcgggcc ttcttgagac
     3481 cggccttctt gcgctccttc acccgggcat cgcgggtgag caggccggcc ttcttcaggg
     3541 ccggacggga ggcctcggca tccactgcgt tgagcgcgcg ggcgatgccg agacgcaggg
     3601 caccggcctg gccggtgacc ccgccgccga cgatcttggc gatcacgtcg tagctgccgg
     3661 cgaccgctgc agtctcgaag ggctcgttga cggtctgctg gtgcagcttg ttcggaaagt
     3721 actcctccag cgtgcggccg ttgaccttcc actggcccgt gccaggggtg aggcggacgc
     3781 gcgcgatggc ctccttgcga cggccggtgg catcggcggc gccgatcacg gcgggccgaa
     3841 cgcccggggc ggcggacgag ggcacggctt cggcgcggta ggcgatctcg cggtcaccct
     3901 cggtgaacgg ggcgaggtcc tcggtggtct cgacggtctc gacggtctcg gtcactgtgc
     3961 ttccttcggc tctcactgag cgatctgggt gatctggtac ggctgcggct gctgcgcggt
     4021 gtgcggatgc tcaggacccg agtacacctt cagcttcttg atcagctggc ggctcagctt
     4081 gttcttgggc agcatgcccc acacggcgcg ctcgacggcg ttgcgcgggt cggtggccag
     4141 cagctcgccg tacggaacgg ccttcaggcc acccggacga ccggagtgcc ggtacgccat
     4201 cttgttggtg cgcttgctgc cggtcagcgc gatcttggac gcgttgacga cgatcacgaa
     4261 gtcaccggtg tcgagatgcg gagcgaactg tgccttgtgc ttgccgcgca gcagggtggc
     4321 ggccgtgacg gcgaggcgcc cgagaacgac atcggaggca tcgatcacat gccagttacg
     4381 ggtgacctca cccgacttgg ggctgtacgt agacacgctc gtggaccact tccagttgat
     4441 aacagtttgt cggcagtgcg ggatctgcca gaacagacct cagcgcaaca gcgattcaga
     4501 ttacctgccg gggctgggtc gggtcaaaac gacggccgga cggggccgcc ccgcacccgg
     4561 ctcgcgccga catcacagcg gctgcacagt gcggtcccag tgcgacgcca gcccgtccgc
     4621 gcatcgtctc ttgggtgacc acctcgacga tcgactcacc cgcgaacgaa gcccgctccc
     4681 ccgggcctcc cgccgccgac accccggcgg ccacgacacc agagcgcgga ccctccaccc
     4741 tggtgcggcg cgcttcgctc acgctcaccc tgctggccgt gctggccctc tacacctgga
     4801 acctcgccgc ctccggctac gcgaactcct tctactccgc ggccgcacag gccggcgccg
     4861 agtcgtggaa ggccttcttc tacggctccc tggacgccgg taactcgatc accgtcgaca
     4921 agaccccggg cgcgttgtgg ctgatggccc tgtcggtgcg cctgttcggg ctcagctcgt
     4981 ggagcatcct gatgccccag gcgctgctcg gcgtcgcctc ggcgtggatc ctgtacggca
     5041 ccgtccgacg ctcgctcgcc ggccccctcg gcaaacgcct gaatgatgcc gtgaccagcc
     5101 gggacgccgc gctcgaaccg atctcggcga cccgtgcgca ctgggccggc atcgtcgcgg
     5161 cgttggcctt cg
//
LOCUS       DAOSUG010000182         5163 bp    DNA     linear   ENV 10-JUN-2024
DEFINITION  MAG TPA_asm: Micropruina sp. isolate AMR_MDS_2195
            NODE_7160_length_5163_cov_4.842013, whole genome shotgun sequence.
ACCESSION   DAOSUG010000182 DAOSUG010000000
VERSION     DAOSUG010000182.1
DBLINK      BioProject: PRJNA925477
            BioSample: SAMN32864262
            Sequence Read Archive: SRR4236673
KEYWORDS    WGS; ENV; Metagenome Assembled Genome; MAG; Third Party Data; TPA;
            TPA:assembly.
SOURCE      Micropruina sp. (wastewater metagenome)
  ORGANISM  Micropruina sp.
            Bacteria; Bacillati; Actinomycetota; Actinomycetes;
            Propionibacteriales; Nocardioidaceae; Micropruina.
REFERENCE   1  (bases 1 to 5163)
  AUTHORS   Abdulkadir,N., Saraiva,J.P., Zhang,J., Stolte,S., Gillor,O.,
            Harms,H. and Rocha,U.
  TITLE     Genome-centric analyses of 165 metagenomes show that mobile genetic
            elements are crucial for the transmission of antimicrobial
            resistance genes to pathogens in activated sludge and wastewater
  JOURNAL   Microbiol Spectr 12 (3), e0291823 (2024)
   PUBMED   38289113
REFERENCE   2  (bases 1 to 5163)
  AUTHORS   Abdulkadir,N.
  TITLE     Direct Submission
  JOURNAL   Submitted (24-MAR-2023) Environmental Microbiology, Helmholtz
            Center for Environmental Research -UFZ, Permoserstr 15, Leipzig
            04318, Germany
COMMENT     The annotation was added by the NCBI Prokaryotic Genome Annotation
            Pipeline (PGAP). Information about PGAP can be found here:
            https://www.ncbi.nlm.nih.gov/genome/annotation_prok/
            
            ##Genome-Assembly-Data-START##
            Assembly Method        :: SPAdes v. 3.11.1
            Genome Representation  :: Full
            Expected Final Version :: Yes
            Genome Coverage        :: Not Applicable
            Sequencing Technology  :: Illumina MiSeq
            ##Genome-Assembly-Data-END##
            
            ##Genome-Annotation-Data-START##
            Annotation Provider               :: NCBI
            Annotation Date                   :: 05/04/2023 04:57:10
            Annotation Pipeline               :: NCBI Prokaryotic Genome
                                                 Annotation Pipeline (PGAP)
            Annotation Method                 :: Best-placed reference protein
                                                 set; GeneMarkS-2+
            Annotation Software revision      :: 6.5
            Features Annotated                :: Gene; CDS; rRNA; tRNA; ncRNA
            Genes (total)                     :: 2,776
            CDSs (total)                      :: 2,750
            Genes (coding)                    :: 2,714
            CDSs (with protein)               :: 2,714
            Genes (RNA)                       :: 26
            tRNAs                             :: 24
            ncRNAs                            :: 2
            Pseudo Genes (total)              :: 36
            CDSs (without protein)            :: 36
            Pseudo Genes (ambiguous residues) :: 0 of 36
            Pseudo Genes (frameshifted)       :: 2 of 36
            Pseudo Genes (incomplete)         :: 32 of 36
            Pseudo Genes (internal stop)      :: 2 of 36
            ##Genome-Annotation-Data-END##
FEATURES             Location/Qualifiers
     source          1..5163
                     /organism="Micropruina sp."
                     /mol_type="genomic DNA"
                     /submitter_seqid="NODE_7160_length_5163_cov_4.842013"
                     /isolate="AMR_MDS_2195"
                     /isolation_source="Activated sludge of municipal
                     wastewater treatment plants"
                     /db_xref="taxon:2737536"
                     /environmental_sample
                     /geo_loc_name="Canada"
                     /lat_lon="49.25 N 123.1 W"
                     /collection_date="2015-06-16"
                     /metagenome_source="wastewater metagenome"
                     /note="metagenomic"
     gene            <1..1378
                     /locus_tag="PLF56_07145"
     CDS             <1..1378
                     /locus_tag="PLF56_07145"
                     /inference="COORDINATES: protein motif:HMM:NF012602.2"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_function: GO:0016491 - oxidoreductase activity
                     [Evidence IEA]"
                     /codon_start=2
                     /transl_table=11
                     /product="molybdopterin-dependent oxidoreductase"
                     /protein_id="HOQ53375.1"
                     /translation="GTNNIDFRSRPHSAEEAAFLANRVAGRALDTDTLSYTALQGAKQ
                     VILVAFEPEEEAAMVFLRLRKAVRKNGLKVVSVAPYASNGSVKLNAELVATVPGTEPE
                     VLAGLSADADTIVLVGERAAAIPGALTAAADFAERTGARLGWIPRRAGDRGAVEAGCL
                     PNLLPGARPAGEVLARVDVQAAWGVDGLPTLPGLDADAQLAAAAREGLALVVAGIEPS
                     DFTNAAEVREGLEAAPFVVSLEQRLSEVTERADVVFPIALIEERPGHFMNWEHRRGRV
                     NTVIEQPNQPMTDLRVLAALAEALGRPLGIRTARQALTELDELGVWDGERQPLARTRG
                     VAAPAPDELALATWRELIDGSRGNDNEPALLATAKAVVARTSPEVADAHGLSDAVTIA
                     GNGGWLTLPLEIVPGMAPDTVWVPTHAPGTPLSELGLVHGDSVTVSSPAAALDRGDGF
                     DRGEGGVA"
     gene            1375..2688
                     /gene="nuoH"
                     /locus_tag="PLF56_07150"
     CDS             1375..2688
                     /gene="nuoH"
                     /locus_tag="PLF56_07150"
                     /EC_number="1.6.5.9"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_013160445.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_component: GO:0016020 - membrane [Evidence IEA];
                     GO_function: GO:0016651 - oxidoreductase activity, acting
                     on NAD(P)H [Evidence IEA];
                     GO_function: GO:0051539 - 4 iron, 4 sulfur cluster binding
                     [Evidence IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="NADH-quinone oxidoreductase subunit NuoH"
                     /protein_id="HOQ53376.1"
                     /translation="MIPLDLSLFGTDPWWLVLLKVVVLFVVLLVWTIFNVWYERRLVG
                     KMQHRLGPIMNGPFGLGQALADGMKLLIKEDFRPKSADRVLFTLAPLIAGTAAFSAWA
                     VIPFGGEVEIFGVRTRLQGTDLPISVLVILAVASVGIYGFVLAGWSSNSPYSLLGALR
                     STAQMISYELSMGLALVAVFLYSKSMSTSEIVAAQGQPLLIFGHDIGLQGWYFALLLP
                     SFVVYVISMVGETNRAPFDLPECESELVSGYLTEYSGFRYATYFLAEYINMATVSAVA
                     TTLFLGGYHAPWPINWLIPAMDQGWWGPLWFVLKVQLLISLFVWLRGTLPRFRYDQFM
                     NLGWKWLIPISLVWVMLIATFRIARATGWLSNPLVIGGTLVVVLALFAIWWLLDREPE
                     PALPDDSGTFDAFAGGYPVPPMPGQKLPELADVVPSKLDAERTDA"
     gene            2688..3248
                     /gene="nuoI"
                     /locus_tag="PLF56_07155"
     CDS             2688..3248
                     /gene="nuoI"
                     /locus_tag="PLF56_07155"
                     /EC_number="1.6.5.9"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_015071302.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_function: GO:0016651 - oxidoreductase activity, acting
                     on NAD(P)H [Evidence IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="NADH-quinone oxidoreductase subunit NuoI"
                     /protein_id="HOQ53377.1"
                     /translation="MASFADQWAGFGVTFRTMFRKTFTQGYPEKGKVKVTAPRFHGRH
                     QLNRWPDGLEKCVGCELCAWACPADAIYVEGADNTEDARFSPGERYGSVYQINYLRCI
                     LCGLCIEACPTRALTMTNEFKLGDTTREKMIYTKDRLLAPLLPGMEAPPHPRRLGDDE
                     RTYFLGLPKTGTPDTRTSDVTAGEGR"
     gene            3245..4030
                     /locus_tag="PLF56_07160"
     CDS             3245..4030
                     /locus_tag="PLF56_07160"
                     /EC_number="1.6.5.9"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_015071301.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_function: GO:0008137 - NADH dehydrogenase (ubiquinone)
                     activity [Evidence IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="NADH-quinone oxidoreductase subunit J"
                     /protein_id="HOQ53378.1"
                     /translation="MNVVFWICAPLMVLGGLGLILFRKAVYSALSLALTMVCLAVLYA
                     SLEAPFLFAIQIIVYTGAVLMLFLFVLMMVGVDTPDSIVETIKGQRILAALGALGLLG
                     LMVFSIGGAITTPSDGMAGATEAAGGNVQGVAQLLFGRYVWVFELTSALLITAAVGAM
                     LLAHAQRTKPKLGQREHAEQRMKAYADRGAHPGSLPNSGVFATSNSIATPALLPDGSV
                     SEESISTTLTERGAQLDSTRLREITAEAFADAQRVRNEEDEEL"
     gene            4027..4323
                     /gene="nuoK"
                     /locus_tag="PLF56_07165"
     CDS             4027..4323
                     /gene="nuoK"
                     /locus_tag="PLF56_07165"
                     /EC_number="1.6.5.9"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_007076572.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_function: GO:0016651 - oxidoreductase activity, acting
                     on NAD(P)H [Evidence IEA];
                     GO_process: GO:0042773 - ATP synthesis coupled electron
                     transport [Evidence IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="NADH-quinone oxidoreductase subunit NuoK"
                     /protein_id="HOQ53379.1"
                     /translation="MIDQYLYLSVILFVIGAIGVMVRRNALVAFMSVELMLNAANLAL
                     VAFAWHHASFDGQVATFFVMVVAAAEVVVGLAIIVSIYKTRRSTSVDNANLLNQ"
     gene            4349..>5163
                     /locus_tag="PLF56_07170"
     CDS             4349..>5163
                     /locus_tag="PLF56_07170"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_013861710.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="proton-conducting transporter membrane subunit"
                     /protein_id="HOQ53380.1"
                     /translation="MMPLEIVDPVAATGLFSYAWLMIALPALGAFVLLAFGRFVEKSG
                     PYLAVATITGSFVIAVALFVQLLGSPVEDRAVGVHLYTWFATGSWQIDVGLLVDQLSI
                     LFALLITGVGGLIHVYSLGYMAHDERKRRFFGYLNLFVAAMLTLVLADNYLVLFVGWE
                     GVGLASYLLIGFWQHKPSAAAAAKKAFVMNRVGDLGMVLAISTMLAQFGSSAFVDVNH
                     GAANLAGPWPMVIGLLLLLGACGKSAQVPLQAWLLDAMEGPTPVSALIHAATMV"
BASE COUNT          718 a         1765 c         1774 g          906 t
ORIGIN      
        1 gggcacgaac aacatcgact tccgctcgcg tccgcacagc gcggaggagg ccgccttcct
       61 ggccaaccgg gtggcgggcc gcgcgctcga taccgacacc ctcagctaca ccgcgctgca
      121 gggcgccaaa caggtgatcc tggtcgcctt cgagcccgaa gaagaggccg cgatggtctt
      181 tctgcggctg cgcaaggcgg tgcgcaagaa cgggctcaag gtggtgagcg tcgcgccgta
      241 cgcgtccaac ggttcggtga agctgaacgc cgagttggtc gcgaccgtgc cgggcaccga
      301 gcctgaggtg ctggccgggc tgagcgccga cgccgacacg atcgtgctgg tgggcgagcg
      361 ggcagcggcg attcccggcg cgctcaccgc cgccgccgac ttcgcagagc gcaccggggc
      421 gcggctgggc tggatcccgc gccgtgccgg tgaccgcggc gccgtcgagg cggggtgcct
      481 gcccaatctg ttgccgggcg cgcgtccggc cggtgaggtg ctggcccggg tcgacgtgca
      541 ggccgcctgg ggtgtggacg gcctgccgac actgcccggc ctggacgccg acgcccagct
      601 cgccgccgcg gcccgtgaag gcctcgcgct ggtggtggcc ggcatcgagc cgtccgactt
      661 caccaacgcg gccgaggtgc gcgaggggtt ggaggccgca ccgttcgtgg tcagcctcga
      721 acagcgcctc tccgaggtga ccgagcgtgc cgacgtggtg ttcccgatcg ccctgatcga
      781 ggagcgtccc gggcatttca tgaactggga gcaccggcgc ggccgcgtca acaccgtgat
      841 cgagcagccc aaccagccga tgaccgacct gcgggtgctg gccgcgctgg ccgaggcgct
      901 gggccgtccg ctgggcatcc gtaccgcccg gcaggccctg accgagctgg acgaactcgg
      961 cgtctgggac ggcgagcggc agccgctggc ccgcacccgc ggcgtcgcgg ccccggcccc
     1021 ggacgaactg gccctggcca cctggcgtga actgatcgac ggctcgcgcg gcaacgacaa
     1081 cgagccggcg ctgctggcca cggccaaggc cgtcgtggca cgcacctcgc ccgaggtggc
     1141 ggacgcgcac gggctgagcg acgcagtgac catcgccggt aacggcggct ggctcaccct
     1201 tccgctcgag atcgtgcccg gcatggcgcc cgacacggtg tgggtgccca cccacgcgcc
     1261 cggtacgccg ctgtcggagt tgggcctggt gcacggcgac agcgtcacgg tcagctcgcc
     1321 ggctgcggcg ctcgaccgag gcgacggctt cgatcgcggt gagggaggcg tggcatgatt
     1381 ccgctcgatc tttcgttgtt cggcaccgac ccgtggtggc tggtgctgct caaggtcgtg
     1441 gtgctgttcg tggtgctgct ggtctggacg atcttcaacg tctggtacga gcggcgcctc
     1501 gtgggcaaga tgcagcatcg gctcggcccc atcatgaacg gcccgttcgg cctcggccag
     1561 gccctggccg acggcatgaa gctgctgatc aaagaggact tccggcccaa gtcggccgac
     1621 cgggtgctgt tcacgctcgc gccgctgatc gccggcaccg ccgccttctc ggcgtgggcg
     1681 gtgattccct tcggcggcga ggtcgagatc ttcggggtac gcacccggct gcagggcacc
     1741 gacctgccga tctcggtgct ggtgatcctg gcggtcgcct cggtgggcat ctacggcttc
     1801 gtgctggccg ggtggtcgtc caactcgccc tattcgctgc tgggtgcgct acgttccacc
     1861 gcgcagatga tcagctacga gctcagcatg ggcctggccc tggtcgcggt gttcctgtac
     1921 agcaaatcga tgtcaacctc cgagatcgtg gccgcccagg ggcagccact gctgatcttc
     1981 ggtcacgaca tcggcctgca gggttggtac ttcgcgttgc tgctgccgtc gttcgtggtc
     2041 tatgtgatct cgatggtggg cgaaaccaac cgggcgccct tcgacctgcc cgagtgcgaa
     2101 tcggagctgg tctcgggcta cctcaccgag tacagcggct ttcgctacgc cacctacttt
     2161 ctcgccgagt acatcaacat ggccaccgtg tcggccgtcg ccaccacgct gttcctgggc
     2221 ggctaccacg cgccgtggcc gatcaactgg ctgatcccgg cgatggacca aggctggtgg
     2281 ggtccgctgt ggttcgtgct caaggtgcag ttgctgatct cgctgttcgt gtggctgcgc
     2341 ggcaccctgc cccggttccg ctacgaccag ttcatgaacc tgggctggaa gtggctgatt
     2401 ccgatctcgc tggtgtgggt gatgctgatc gccaccttcc gcatcgcacg ggccaccggc
     2461 tggttgagca acccgctggt gatcggcggc accctcgtcg tggtgctggc gttgttcgcg
     2521 atctggtggc tgctcgaccg tgaacccgag cccgcgctgc ccgacgacag cggcaccttc
     2581 gacgccttcg ccggcgggta cccggtgccg ccgatgccgg gccagaagct gccggagttg
     2641 gccgatgtgg tgccctcgaa gctcgatgcc gaaaggacgg acgcctgatg gcctcgttcg
     2701 cagatcagtg ggccggtttc ggcgtcacgt tccgcaccat gtttcgcaag acgttcaccc
     2761 agggctaccc cgagaagggc aaggtcaagg tcaccgcacc ccggttccat ggccggcacc
     2821 agctgaaccg ctggcccgac gggctggaga agtgcgtcgg ctgtgagttg tgcgcctggg
     2881 cctgtccggc ggacgccatc tacgtcgagg gtgccgacaa caccgaggac gcccggttct
     2941 ctcccggcga gcgctacggc tcggtgtacc agatcaacta cctgcgctgc attctgtgcg
     3001 ggctgtgcat cgaggcgtgc cccacccggg cgctgaccat gaccaacgag ttcaagctcg
     3061 gcgacaccac ccgcgagaag atgatctaca ccaaggaccg gctgctcgct ccgctgctgc
     3121 ccggcatgga ggcgccgccg catccgcgcc ggctcggcga cgacgagcgc acctactttc
     3181 tcgggctgcc gaagaccggc acccccgaca cccgcacctc ggacgtgacg gcgggtgagg
     3241 gccgatgaac gtcgtgttct ggatctgcgc gccgctgatg gtgctcggtg gcctcgggct
     3301 gatcctgttc cgcaaggcgg tctattccgc gctgtcgctg gccctgacca tggtgtgcct
     3361 ggccgtgctg tacgcctcgc tcgaggctcc gttcctgttc gcgatccaga tcatcgtcta
     3421 caccggcgcg gtgctgatgc tgttcctgtt cgtgctgatg atggtcggcg tcgacacccc
     3481 cgattcgatc gtcgaaacca tcaaggggca gcgcattctc gccgcactgg gtgcgctggg
     3541 gctgctcggc ctgatggtgt tcagcatcgg aggggcgatc accaccccgt ccgacggcat
     3601 ggcgggcgcc accgaagcgg ccggcggcaa cgtgcagggc gtcgcccagt tgctgttcgg
     3661 acgctacgtg tgggtgttcg agctcacctc ggcgctgctg atcaccgccg cggtcggggc
     3721 catgcttctg gcccacgccc agcgcaccaa gccgaagctc ggccagcgcg agcacgccga
     3781 gcagcgcatg aaggcctacg ccgatcgcgg cgcccacccc ggctcgctgc ccaactcggg
     3841 cgtgttcgcc accagcaact cgatcgccac ccccgcactg ctgcccgacg gcagcgtgtc
     3901 cgaggagtcg atcagcacga ccctgaccga acgcggcgcc caactcgaca gcacccggtt
     3961 gcgtgagatc accgcagagg ccttcgccga cgcccagcgc gtccgcaacg aggaggacga
     4021 ggagctgtga tcgatcagta cctgtacctg tcggtgatcc tgttcgtgat cggcgccatc
     4081 ggcgtgatgg tgcggcgcaa cgcgctcgtc gcattcatgt cggtggagct gatgctgaac
     4141 gcggccaacc tcgccctggt ggccttcgcc tggcatcacg ccagcttcga cggccaggtc
     4201 gccaccttct tcgtgatggt ggtggcggcg gccgaggtgg tcgtcggcct ggcgatcatc
     4261 gtgagcatct acaagacccg ccgctcgacc tcggtcgaca acgcgaacct gttgaaccag
     4321 tgaggctgca caagatgggg aaaagggcat gatgccgctc gaaatcgtcg atccggtcgc
     4381 ggccaccggg ttgttcagct acgcctggct gatgatcgct ctgccggcgc tcggtgcgtt
     4441 cgtgctgctc gcgttcggac gcttcgtcga gaagtccggg ccgtacctgg cggtcgccac
     4501 catcaccggc tcgttcgtga tcgcggtggc gctgttcgtg cagctgctcg gctcgccggt
     4561 ggaggaccgt gcggtcgggg tgcacctgta cacctggttc gccaccggca gctggcagat
     4621 cgacgtcggg ctgctggtcg accagctgtc gatcctgttc gcgctgctga tcaccggcgt
     4681 cggcggtctg attcacgtgt actcgctcgg ctacatggcc cacgacgagc gcaaacgccg
     4741 cttcttcggc tacctgaacc tgttcgtcgc ggccatgctc accctggtgc tggccgacaa
     4801 ctacctggtg ctgttcgtcg gctgggaggg cgtcggcctg gcgtcctacc tgctgatcgg
     4861 gttctggcag cacaagccgt ccgccgccgc tgccgccaag aaggcgttcg tgatgaaccg
     4921 ggtcggcgac ctgggcatgg tgttggccat ctcgacgatg ctggcccagt tcggttcgtc
     4981 cgccttcgtc gacgtcaacc acggcgcggc caacctggcc ggcccctggc cgatggtgat
     5041 cggcctgctg ctgctgctgg gcgcctgcgg caagtcggcg caggtgccgc tgcaggcctg
     5101 gctgctggac gccatggagg gccccacgcc ggtctcggcc ctgatccacg cggcgaccat
     5161 ggt
//
LOCUS       DAOSUG010000183         5153 bp    DNA     linear   ENV 10-JUN-2024
DEFINITION  MAG TPA_asm: Micropruina sp. isolate AMR_MDS_2195
            NODE_7173_length_5153_cov_4.565124, whole genome shotgun sequence.
ACCESSION   DAOSUG010000183 DAOSUG010000000
VERSION     DAOSUG010000183.1
DBLINK      BioProject: PRJNA925477
            BioSample: SAMN32864262
            Sequence Read Archive: SRR4236673
KEYWORDS    WGS; ENV; Metagenome Assembled Genome; MAG; Third Party Data; TPA;
            TPA:assembly.
SOURCE      Micropruina sp. (wastewater metagenome)
  ORGANISM  Micropruina sp.
            Bacteria; Bacillati; Actinomycetota; Actinomycetes;
            Propionibacteriales; Nocardioidaceae; Micropruina.
REFERENCE   1  (bases 1 to 5153)
  AUTHORS   Abdulkadir,N., Saraiva,J.P., Zhang,J., Stolte,S., Gillor,O.,
            Harms,H. and Rocha,U.
  TITLE     Genome-centric analyses of 165 metagenomes show that mobile genetic
            elements are crucial for the transmission of antimicrobial
            resistance genes to pathogens in activated sludge and wastewater
  JOURNAL   Microbiol Spectr 12 (3), e0291823 (2024)
   PUBMED   38289113
REFERENCE   2  (bases 1 to 5153)
  AUTHORS   Abdulkadir,N.
  TITLE     Direct Submission
  JOURNAL   Submitted (24-MAR-2023) Environmental Microbiology, Helmholtz
            Center for Environmental Research -UFZ, Permoserstr 15, Leipzig
            04318, Germany
COMMENT     The annotation was added by the NCBI Prokaryotic Genome Annotation
            Pipeline (PGAP). Information about PGAP can be found here:
            https://www.ncbi.nlm.nih.gov/genome/annotation_prok/
            
            ##Genome-Assembly-Data-START##
            Assembly Method        :: SPAdes v. 3.11.1
            Genome Representation  :: Full
            Expected Final Version :: Yes
            Genome Coverage        :: Not Applicable
            Sequencing Technology  :: Illumina MiSeq
            ##Genome-Assembly-Data-END##
            
            ##Genome-Annotation-Data-START##
            Annotation Provider               :: NCBI
            Annotation Date                   :: 05/04/2023 04:57:10
            Annotation Pipeline               :: NCBI Prokaryotic Genome
                                                 Annotation Pipeline (PGAP)
            Annotation Method                 :: Best-placed reference protein
                                                 set; GeneMarkS-2+
            Annotation Software revision      :: 6.5
            Features Annotated                :: Gene; CDS; rRNA; tRNA; ncRNA
            Genes (total)                     :: 2,776
            CDSs (total)                      :: 2,750
            Genes (coding)                    :: 2,714
            CDSs (with protein)               :: 2,714
            Genes (RNA)                       :: 26
            tRNAs                             :: 24
            ncRNAs                            :: 2
            Pseudo Genes (total)              :: 36
            CDSs (without protein)            :: 36
            Pseudo Genes (ambiguous residues) :: 0 of 36
            Pseudo Genes (frameshifted)       :: 2 of 36
            Pseudo Genes (incomplete)         :: 32 of 36
            Pseudo Genes (internal stop)      :: 2 of 36
            ##Genome-Annotation-Data-END##
FEATURES             Location/Qualifiers
     source          1..5153
                     /organism="Micropruina sp."
                     /mol_type="genomic DNA"
                     /submitter_seqid="NODE_7173_length_5153_cov_4.565124"
                     /isolate="AMR_MDS_2195"
                     /isolation_source="Activated sludge of municipal
                     wastewater treatment plants"
                     /db_xref="taxon:2737536"
                     /environmental_sample
                     /geo_loc_name="Canada"
                     /lat_lon="49.25 N 123.1 W"
                     /collection_date="2015-06-16"
                     /metagenome_source="wastewater metagenome"
                     /note="metagenomic"
     gene            <1..533
                     /locus_tag="PLF56_07175"
     CDS             <1..533
                     /locus_tag="PLF56_07175"
                     /inference="COORDINATES: ab initio
                     prediction:GeneMarkS-2+"
                     /note="Derived by automated computational analysis using
                     gene prediction method: GeneMarkS-2+."
                     /codon_start=3
                     /transl_table=11
                     /product="hypothetical protein"
                     /protein_id="HOQ53381.1"
                     /translation="DVEIQYSDTSGTRYNERSVLDLAATKGALTIRVKSLHDIGNTLE
                     KIERLLDRATVLGRDGYIEVASVVETFDEHRLRLEAEEAENDKQRLLWMERYRPASPE
                     TAQLRAVVAARAESAVPPSAGSQTSSTPAPDAPEVESAMVRDHSRRNGRRRNLTAQID
                     VRGMWWLQTTRSTRLR"
     gene            complement(720..796)
                     /locus_tag="PLF56_07180"
     tRNA            complement(720..796)
                     /locus_tag="PLF56_07180"
                     /product="tRNA-Pro"
                     /inference="COORDINATES: profile:tRNAscan-SE:2.0.12"
                     /note="Derived by automated computational analysis using
                     gene prediction method: tRNAscan-SE."
                     /anticodon=(pos:complement(760..762),aa:Pro,seq:cgg)
     gene            complement(840..1745)
                     /locus_tag="PLF56_07185"
     CDS             complement(840..1745)
                     /locus_tag="PLF56_07185"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_015071581.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_function: GO:0016787 - hydrolase activity [Evidence
                     IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="metallophosphoesterase"
                     /protein_id="HOQ53382.1"
                     /translation="MIARALGRVALVGAACVGYGFWEARHFVVRRQSVPILPAGSAPI
                     RLLHVSDIHLMARQRYKTEFLSALAGLEPDLVVTTGDNWCEKQALEPLLRTWGRLRDV
                     PGVFVFGSNDYEAPTFRPPMAYLLRSTEGREGGKKRPLPVDELRAGYSERGWLDVDTT
                     RAQLTVRGVTFEFRGTDDAHLGRDDYALVAGPASPGADVSIGVTHAPYLRVLDAMTAD
                     GVQLMLAGHTHGGQVCVPYYGALVTNCDLDPIRAKGLSTHTADGRTSYLHVSAGMGSS
                     PFATYRFACPPEVTVLTLTAPDANG"
     gene            complement(1747..4128)
                     /locus_tag="PLF56_07190"
     CDS             complement(1747..4128)
                     /locus_tag="PLF56_07190"
                     /inference="COORDINATES: protein motif:HMM:NF013100.2"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="transglycosylase domain-containing protein"
                     /protein_id="HOQ53383.1"
                     /translation="MRLGKRLYSLVMFVVVSVLGGLLIAGLVVPAAGVAAEVGKTTAS
                     AMDALPAELAEQPLAQKTKVLLADGSTLVNFYQENRQYVPLSKIAPIMQQAQIAIEDH
                     RFYQHGAMDLQGTMRALVSTVRGNTQGASTLTQQYVKMSLINAAQAKGDTEGVQKAQE
                     NTVARKIQELRYAIALEKKYTKDQILEFYLNIAYYGDGAYGVEAAALNYFGIHASKLN
                     LQQAAFLAGMVRNPVGTNPRTPGLELVALQRRNDVLDRMASTEVHVITPQQAAAAKAT
                     KLGLSKPTKSLHNCANSQFPFICDYVERTLKASPALGKTVEDRANAIYRGGLTIRTTI
                     DPKVQLKSEKTITNYLDPRDPVDAVIAMIEPGTGKIVSMAQNRTKMGTKKSETYYNYA
                     ANSKLGGGAGYRGGSTFKMFVAAAALQNGLGAYGNIRVQRSMNFQGWRFDACSGSYIQ
                     PSRWNVVGEAGTFNLFSGVQKSVNGYFVQLESAAGLCDAAKTAKALGLQLSNDPYGDE
                     LLKEAYASFTLGSVDVAPLSLVNAYATVAARGIHCDPIIISSIKASDGTTIEPPSANC
                     KRVMDEGIADALSKIFQGPWNPGGTAYPADIPGYQIAGKTGTVDSNRSIWTIGFTRNL
                     AGGAMISYSSDPRFRSYWSGYRGRNYLRGAYMKYSHRVLQGASGREAGRGLLHSALAY
                     GLKQFPKGPGFVDPPYGILAGQKVDVPSCSGSADCKTTLRRAGFGYYVDSITSNSPKG
                     TFLGLSPSTVAVKGTSIRILISSGPAPKKPDKKKDDDKPSSSPKPSPAPTKTR"
     gene            4364..4633
                     /locus_tag="PLF56_07195"
     CDS             4364..4633
                     /locus_tag="PLF56_07195"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_002517165.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_function: GO:0003677 - DNA binding [Evidence IEA];
                     GO_function: GO:0046872 - metal ion binding [Evidence
                     IEA];
                     GO_function: GO:0051536 - iron-sulfur cluster binding
                     [Evidence IEA];
                     GO_function: GO:0051539 - 4 iron, 4 sulfur cluster binding
                     [Evidence IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="WhiB family transcriptional regulator"
                     /protein_id="HOQ53384.1"
                     /translation="MSVMEMDSWPLLAKCRGMQDDLFPDGADQKRARNICAGCQVRAE
                     CLAEALDNRIEWGVWGGMTERERRRLLRQHGDITSWRRLLLDPSR"
     gene            complement(4671..>5153)
                     /locus_tag="PLF56_07200"
     CDS             complement(4671..>5153)
                     /locus_tag="PLF56_07200"
                     /inference="COORDINATES: ab initio
                     prediction:GeneMarkS-2+"
                     /note="Derived by automated computational analysis using
                     gene prediction method: GeneMarkS-2+."
                     /codon_start=1
                     /transl_table=11
                     /product="hypothetical protein"
                     /protein_id="HOQ53385.1"
                     /translation="AALATRALDAILGGRLFGDLRTFLQVFERAVLAFESHAAETRAL
                     LASERAGFLVVAAPRPGPVAEAVELVRRLQADGLPLRGVLVNQVTAVADVSAATPAAA
                     SSVDQDQRIVLDHHERMVARQAVELRAIEPLTATGVPLRRIGRVPGGLTDLRSLAGLV
                     "
BASE COUNT          871 a         1663 c         1735 g          884 t
ORIGIN      
        1 acgacgtgga gatccaatac tccgacacct cgggcacccg ctacaacgaa cgaagcgtcc
       61 tcgacctagc ggcaaccaag ggcgctctca ctatccgagt gaagtcactg cacgacattg
      121 gcaacacact cgagaagatc gaacgtctcc tcgaccgcgc gacggtgctc ggtcgagacg
      181 gttacatcga agtggcgtcc gttgtcgaga cattcgacga acatcgactc cgcctggagg
      241 cagaagaggc tgagaacgat aagcaacgac tcctgtggat ggagcgatat cggcccgcct
      301 ctcccgaaac cgcccagctc cgggcagtcg tggccgcacg cgccgagagc gcggtgccac
      361 cgtccgcagg ttcacaaacg tcctcgaccc cggcacccga cgcacctgag gtcgagtctg
      421 caatggtccg ggaccactcc cgtcgcaacg ggaggcgacg caacctgact gcacagattg
      481 atgtccgggg catgtggtgg ctccagacaa cgagaagcac aaggttgcgg tagtgggctc
      541 cttcaggcgt tcggaccctg gcgtggccgc agccatggag cggacaagag ggattatgcg
      601 gcgtgacagt gtgcgcagac gtccgcacgg ctctgccgtg cttagggtgc gcgggggatg
      661 caagacgccg aaagcctctc caactgggac tattatccct agtcagagcg gctttccgct
      721 ggtcggggtg acaggatttg aacctgcgac ctcgtcgtcc cgaacgacgc gcgctaccaa
      781 gctgcgccac accccgatgg cccgaaggcc gtggagcagt ctatacgaga ccgcaggctc
      841 tagccattcg cgtccggcgc ggtgagcgta agcaccgtca cctcgggcgg gcaggcgaac
      901 cggtaggtgg cgaatggtga ggagcccatg ccggcggaga cgtgcaggta cgaggtgcgt
      961 ccgtccgcag tgtgcgtgga caggcccttg gcgcgaatcg ggtcgaggtc gcagttggtc
     1021 accagcgccc cgtaataggg cacgcacacc tggccaccgt gggtgtggcc ggccagcatc
     1081 aactggacgc cgtcggcggt catcgcgtcc agcacccgca ggtagggggc gtgggtgacg
     1141 ccgatcgaga cgtcggcgcc gggggatgcc gggccggcca ccagcgcgta gtcgtcgcgg
     1201 ccgaggtggg cgtcgtcggt gccgcgaaac tcgaacgtca cgccccggac ggtgagttgt
     1261 gcgcgggtcg tgtcgacgtc cagccagccg cgttcgctgt agccggcccg cagttcgtcg
     1321 accggcagcg gacgcttctt accgccctcg cgtccctcgg tggaacgcag caagtaggcc
     1381 atcggcggac ggaacgtggg ggcctcgtag tcgttggagc cgaagacgaa gacgccgggc
     1441 acgtcacgca gccgtcccca ggtgcgcagc aacggttcga gcgcctgctt ctcacaccag
     1501 ttgtctccgg tggtgaccac cagatcgggc tccagcccgg ccagcgcact cagaaactcg
     1561 gtcttgtagc gttggcgcgc catcagatga atatccgaga catgcagcag ccggatcgga
     1621 gccgaaccgg ccggcagaat cggcacgctc tggcggcgta cgacgaagtg tcgcgcctcc
     1681 cagaagccgt acccgacgca cgcggctccg accaacgcca cccggcccag ggcgcgcgcg
     1741 atcacctcag cgggtcttgg tcggtgccgg gctcggcttg ggcgacgaac tgggcttgtc
     1801 gtcgtccttc ttcttgtccg gcttcttggg tgccgggccg ctcgagatca ggatgcgaat
     1861 gctggtgccc ttcaccgcca ccgtggacgg gctgaggccg aggaacgtgc ccttgggcga
     1921 gttgctggtg atcgagtcga cgtagtaccc gaagcccgcc cggcgcagcg tcgtcttgca
     1981 gtcggcggag cccgagcagc tgggcacgtc gaccttttgg ccggccagga tgccgtaggg
     2041 cgggtcgacg aagccgggac ccttggggaa ctgcttcagc ccgtacgcaa gggccgaatg
     2101 cagcaggccg cggcccgcct cacgtccgga cgccccctgc agaacgcggt gggagtactt
     2161 catgtacgcg ccccgcaggt agttacgccc gcggtagccg ctccagtagc tgcggaaccg
     2221 cggatcgctg gagtagctga tcatggcgcc gccggccagg ttgcgggtga aaccgatcgt
     2281 ccagatggag cggttgctgt cgacggtgcc ggtcttgccg gcgatctggt aaccgggaat
     2341 gtcggccggg taggccgtgc cgcccgggtt ccaggggcct tggaagatct tcgacagcgc
     2401 gtcggcgatg ccctcgtcca tcacgcgctt gcagttcgcg ctgggcggct cgatcgtggt
     2461 gccgtcgctg gccttgatgg acgagatgat gatcgggtcg cagtgaatgc cgcgcgccgc
     2521 gacggtggcg taggcgttca ccagcgacag cggggccacg tcgaccgagc ccagggtgaa
     2581 cgaggcgtag gcctctttga gcaactcgtc gccgtacggg tcgttgctga gttgcaggcc
     2641 gagcgccttg gcggtcttgg ccgcgtcaca caggccggcc gccgactcca gttgcacgaa
     2701 gtagccgttc accgatttct gcacgccgct gaacaggttg aacgtgccgg cctcgccgac
     2761 cacgttccag cgagagggtt gaatgtacga gccggagcag gcatcgaagc gccacccctg
     2821 aaagttcatg ctgcgctgta cccgaatgtt gccgtaggcc cccaagccgt tctgcaacgc
     2881 tgccgccgcc acgaacatct tgaacgtgga gccgccgcgg tacccggcgc cgccgccgag
     2941 cttgctgttc gccgcgtagt tgtaatacgt ctcgctcttc ttggtgccca tcttggtgcg
     3001 gttctgggcc atcgagacga tcttgcccgt gcccggctcg atcatggcga tcacggcgtc
     3061 caccggatcg cggggatcca gatagttggt gatcgtcttc tcggacttca gctgcacctt
     3121 cgggtcgatg gtggtgcgaa tggtgaggcc gcctcggtag atggcgttgg cgcggtcctc
     3181 gaccgtcttg cccagggcgg gtgaggcctt gagggtgcgc tcgacgtagt cgcagatgaa
     3241 ggggaactgg ctgttggcgc agttgtgcag gctcttggtc ggcttcgaca gcccgagctt
     3301 ggtggccttg gccgccgcag cctgctgcgg ggtgatcacg tgcacctcgg tggacgccat
     3361 gcggtcgagc acgtcgttgc ggcgctgcag tgcgacgagc tcgagccccg gggtgcgcgg
     3421 gttggtgccg acggggttgc ggaccatgcc ggccaggaat gccgcctgct gcaggttcag
     3481 tttggaggcg tggatgccga aatagttgag tgccgcggct tcgactccgt aggcgccgtc
     3541 accgtagtag gcgatgttca ggtagaactc gaggatctgg tccttggtgt acttcttctc
     3601 gagtgcaatc gcgtaccgca actcttggat cttgcgcgca acggtgttct cttgcgcctt
     3661 ctggacgccc tcggtgtcgc ccttggcctg cgcagcgttg atcagactca tcttcacgta
     3721 ctgctgggtg agcgtcgagg cgccctgggt gttgccccgc acggtgctga ccagtgcacg
     3781 catggtgccc tgcaggtcca tcgcgccgtg ttggtagaac cggtgatcct cgatggcgat
     3841 ctgggcctgc tgcatgatcg gggcgatctt gctcaagggc acgtactggc gattctcctg
     3901 atagaagttc accagggtcg acccgtcggc cagcagcact ttcgtcttct gggccagcgg
     3961 ctgctcggcc aactcggcgg gcagggcgtc catggcgctg gctgtggtct tgccgacctc
     4021 ggcggccacg ccggcggccg gtacgaccag ccccgctatc aacaaaccac cgagcacgct
     4081 gaccacgacg aacatgacca gcgagtagag cctcttcccc aaacgcatgg gcaccagact
     4141 acgtgacgct tcagaagccc ccggtatcga ctcctgaggc gttcatgaag gttccgtgcc
     4201 aaccgtgacc aacgagagaa tcatcggttc gcgacaccaa atctgatgaa tctccaacca
     4261 tttggtcgaa attggcggtc ctgaactttg caggccgcaa gtcaagcact acgctgagta
     4321 gtagatgctc acccctgagc tcccccgaag caggtggtac gccttgtcag tcatggagat
     4381 ggacagttgg cctctgttgg ccaagtgccg cggtatgcag gacgatctgt tccccgacgg
     4441 tgccgaccag aagcgcgcgc gcaacatctg tgccggttgc caggttcggg ccgagtgtct
     4501 ggctgaagcc ctcgacaatc gcatcgaatg gggagtgtgg ggcggcatga ccgaacgcga
     4561 gcggcgacgc ctgctgcgcc agcacggcga catcacctca tggcgccggc tgctgctcga
     4621 cccctcgcgc tgagacggag tcaagccaac tagggcagcc ctagaccgcc ctagaccaac
     4681 ccagccaacg agcgcaggtc ggtgagcccg ccgggcaccc ggccgatacg ccgcagcggt
     4741 accccggtcg cggtgagggg ctcgatggca cgcagttcca cagcctgcct ggccaccatt
     4801 cgctcgtgat ggtcgagcac gattcgttga tcctgatcga cggacgacgc cgccgccggc
     4861 gtggcggcag acacgtcggc gaccgccgtg acctgattca ccagcacccc gcgcagcggc
     4921 aggccgtccg cctggagccg ccgcaccagt tccaccgcct cggccaccgg ccccggccgt
     4981 ggcgcggcga ccaccaggaa ccccgcccgc tcggacgcca gtagggcgcg agtctcggcg
     5041 gcgtgggatt cgaaggccag caccgcccgc tcgaacacct gcagaaacgt tctcagatcg
     5101 ccgaagagcc gtccgcccag gatcgcatcc agggcccggg tggcgagtgc agc
//
LOCUS       DAOSUG010000184         5150 bp    DNA     linear   ENV 10-JUN-2024
DEFINITION  MAG TPA_asm: Micropruina sp. isolate AMR_MDS_2195
            NODE_7179_length_5150_cov_4.316781, whole genome shotgun sequence.
ACCESSION   DAOSUG010000184 DAOSUG010000000
VERSION     DAOSUG010000184.1
DBLINK      BioProject: PRJNA925477
            BioSample: SAMN32864262
            Sequence Read Archive: SRR4236673
KEYWORDS    WGS; ENV; Metagenome Assembled Genome; MAG; Third Party Data; TPA;
            TPA:assembly.
SOURCE      Micropruina sp. (wastewater metagenome)
  ORGANISM  Micropruina sp.
            Bacteria; Bacillati; Actinomycetota; Actinomycetes;
            Propionibacteriales; Nocardioidaceae; Micropruina.
REFERENCE   1  (bases 1 to 5150)
  AUTHORS   Abdulkadir,N., Saraiva,J.P., Zhang,J., Stolte,S., Gillor,O.,
            Harms,H. and Rocha,U.
  TITLE     Genome-centric analyses of 165 metagenomes show that mobile genetic
            elements are crucial for the transmission of antimicrobial
            resistance genes to pathogens in activated sludge and wastewater
  JOURNAL   Microbiol Spectr 12 (3), e0291823 (2024)
   PUBMED   38289113
REFERENCE   2  (bases 1 to 5150)
  AUTHORS   Abdulkadir,N.
  TITLE     Direct Submission
  JOURNAL   Submitted (24-MAR-2023) Environmental Microbiology, Helmholtz
            Center for Environmental Research -UFZ, Permoserstr 15, Leipzig
            04318, Germany
COMMENT     The annotation was added by the NCBI Prokaryotic Genome Annotation
            Pipeline (PGAP). Information about PGAP can be found here:
            https://www.ncbi.nlm.nih.gov/genome/annotation_prok/
            
            ##Genome-Assembly-Data-START##
            Assembly Method        :: SPAdes v. 3.11.1
            Genome Representation  :: Full
            Expected Final Version :: Yes
            Genome Coverage        :: Not Applicable
            Sequencing Technology  :: Illumina MiSeq
            ##Genome-Assembly-Data-END##
            
            ##Genome-Annotation-Data-START##
            Annotation Provider               :: NCBI
            Annotation Date                   :: 05/04/2023 04:57:10
            Annotation Pipeline               :: NCBI Prokaryotic Genome
                                                 Annotation Pipeline (PGAP)
            Annotation Method                 :: Best-placed reference protein
                                                 set; GeneMarkS-2+
            Annotation Software revision      :: 6.5
            Features Annotated                :: Gene; CDS; rRNA; tRNA; ncRNA
            Genes (total)                     :: 2,776
            CDSs (total)                      :: 2,750
            Genes (coding)                    :: 2,714
            CDSs (with protein)               :: 2,714
            Genes (RNA)                       :: 26
            tRNAs                             :: 24
            ncRNAs                            :: 2
            Pseudo Genes (total)              :: 36
            CDSs (without protein)            :: 36
            Pseudo Genes (ambiguous residues) :: 0 of 36
            Pseudo Genes (frameshifted)       :: 2 of 36
            Pseudo Genes (incomplete)         :: 32 of 36
            Pseudo Genes (internal stop)      :: 2 of 36
            ##Genome-Annotation-Data-END##
FEATURES             Location/Qualifiers
     source          1..5150
                     /organism="Micropruina sp."
                     /mol_type="genomic DNA"
                     /submitter_seqid="NODE_7179_length_5150_cov_4.316781"
                     /isolate="AMR_MDS_2195"
                     /isolation_source="Activated sludge of municipal
                     wastewater treatment plants"
                     /db_xref="taxon:2737536"
                     /environmental_sample
                     /geo_loc_name="Canada"
                     /lat_lon="49.25 N 123.1 W"
                     /collection_date="2015-06-16"
                     /metagenome_source="wastewater metagenome"
                     /note="metagenomic"
     gene            complement(<1..225)
                     /locus_tag="PLF56_07205"
     CDS             complement(<1..225)
                     /locus_tag="PLF56_07205"
                     /inference="COORDINATES: protein motif:HMM:NF019126.2"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="tail fiber protein"
                     /protein_id="HOQ53386.1"
                     /translation="MTDAYIGEVRCFGFNFAPNDWAQCNGQLLPIQQYTALFSLLGTM
                     YGGNGQTTFGLPDLRGRSAIGAGQGPGLGSY"
     gene            468..1115
                     /locus_tag="PLF56_07210"
     CDS             468..1115
                     /locus_tag="PLF56_07210"
                     /inference="COORDINATES: protein motif:HMM:NF012301.2"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_function: GO:0003677 - DNA binding [Evidence IEA];
                     GO_process: GO:0000160 - phosphorelay signal transduction
                     system [Evidence IEA];
                     GO_process: GO:0006355 - regulation of DNA-templated
                     transcription [Evidence IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="response regulator transcription factor"
                     /protein_id="HOQ53387.1"
                     /translation="MKKRIGIIDDHPAVMLGVASILNAQPDVQVVVTALTAAELVATR
                     QPLDGVLLDLRLADRSTPTDNLRTLAALGAPVLVYTSGEQPELIREAARAGVAGMVRK
                     SELPQVMVEAVRAMLRGEVVASADWAAALDVDPEFVRVSLTAREAEVLELYAAGETAE
                     RVAEALFISRETVLDHVRRIRAKYAAADRPAPTKVDLYRRAVEDGHLVPRHDRLG"
     gene            1099..2265
                     /locus_tag="PLF56_07215"
     CDS             1099..2265
                     /locus_tag="PLF56_07215"
                     /inference="COORDINATES: protein motif:HMM:NF024970.2"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="ATP-binding protein"
                     /protein_id="HOQ53388.1"
                     /translation="MTVSADGPLAPFDRGPSEVERSLGWLIAVGGCILLLDQTVFKVG
                     HAQHFAGWWNAAGVLVAGTLILLAIAGRHLPGRVLRVLWAAVPLGYAMLQAGWSLAYA
                     GTDPNAAMPWLWTLEPAALTLLLLVLTPPSAIAVALAIPLLPALSGWLRFGEVPTAIL
                     HETPSQLGNVVYLALFIGLSEQLKRLSASEAEARRQLQRQHRTTARKRQQDALQRLVH
                     DEVLSVLATAMHADGAPSTALCQDAARAAVALERSLESPGGGSVPADAAVDQVVSQLR
                     RIDPGLALHTQRGDGDFPADAVQALTKAAAEALRNSLAHAGPSSTRRVCVTVDRGELV
                     VVVRDDGIGFDPDPACPRLGIAGSIVGRMRDVGGSARVDSKPTRGTEVVLAWPT"
     gene            2253..3275
                     /locus_tag="PLF56_07220"
     CDS             2253..3275
                     /locus_tag="PLF56_07220"
                     /inference="COORDINATES: ab initio
                     prediction:GeneMarkS-2+"
                     /note="Derived by automated computational analysis using
                     gene prediction method: GeneMarkS-2+."
                     /codon_start=1
                     /transl_table=11
                     /product="hypothetical protein"
                     /protein_id="HOQ53389.1"
                     /translation="MADLTSAGTGAGGLGTPEARWSFIFLLCCGVAGIALGGQWAQAP
                     LLWTLAGLAALAASLLLTTPVRHPLPPSRVVWIPPLALLVAAVALHQTEPHNDFAVLS
                     FASYLVTFLIVRGNVLAGGLGSALVIGYALAWVLPQQPDLRMLGEVLGVPLGGVAAAV
                     AWRLVLRAIVRQERAHRGEAAMAADRARWSALAVRRSEAELTTIRTVVVPLLNRVAAG
                     EPIDATLRAELARTEASVRDRIRVPQLQHPQLVATIEALRLRGVGVLVLGEPQLGEPI
                     GEALALRLHAELEPLQTGRVTVRALPDDRPAAISVLIQDEAGSRRLQFSASGVLLSKG
                     ETVLIR"
     gene            complement(3265..3909)
                     /locus_tag="PLF56_07225"
     CDS             complement(3265..3909)
                     /locus_tag="PLF56_07225"
                     /inference="COORDINATES: protein motif:HMM:NF013933.2"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_component: GO:0016020 - membrane [Evidence IEA];
                     GO_process: GO:0006865 - amino acid transport [Evidence
                     IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="LysE family transporter"
                     /protein_id="HOQ53390.1"
                     /translation="MQVSLWWTLVGAGLLISFTPGAGAISTMANSLAGGWARSIWGVL
                     GQQVALLVHILIVALGVGVLVAANHWLFTAIRWLGAAYLVFLGVRYWLDRPTGVGQLD
                     AEVAQDAERPTYLQPGPMFRRGVLVNLTNPKAIVFFLAFTPQFIRLDGDLLTQYLIYA
                     ATLVTIDIIVMWFFFALAAKALRRFMRDPAGQRLVSRIFGSLFILVGLLLAFIE"
     gene            complement(4054..4233)
                     /gene="rplD"
                     /locus_tag="PLF56_07230"
                     /pseudo
     CDS             complement(4054..4233)
                     /gene="rplD"
                     /locus_tag="PLF56_07230"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_004809054.1"
                     /note="L4 is important during the early stages of 50S
                     assembly; it initially binds near the 5' end of the 23S
                     rRNA; incomplete; partial in the middle of a contig;
                     missing N-terminus; Derived by automated computational
                     analysis using gene prediction method: Protein Homology."
                     /pseudo
                     /codon_start=1
                     /transl_table=11
                     /product="50S ribosomal protein L4"
     gene            complement(4468..4821)
                     /gene="rplQ"
                     /locus_tag="PLF56_07235"
                     /pseudo
     CDS             complement(4468..4821)
                     /gene="rplQ"
                     /locus_tag="PLF56_07235"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_004809007.1"
                     /note="incomplete; partial in the middle of a contig;
                     missing C-terminus; Derived by automated computational
                     analysis using gene prediction method: Protein Homology.
                     GO_component: GO:0005762 - mitochondrial large ribosomal
                     subunit [Evidence IEA];
                     GO_component: GO:0022625 - cytosolic large ribosomal
                     subunit [Evidence IEA];
                     GO_function: GO:0003735 - structural constituent of
                     ribosome [Evidence IEA];
                     GO_process: GO:0006412 - translation [Evidence IEA]"
                     /pseudo
                     /codon_start=1
                     /transl_table=11
                     /product="50S ribosomal protein L17"
     gene            complement(4845..>5150)
                     /locus_tag="PLF56_07240"
     CDS             complement(4845..>5150)
                     /locus_tag="PLF56_07240"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_015071206.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_function: GO:0003677 - DNA binding [Evidence IEA];
                     GO_function: GO:0003899 - DNA-directed 5'-3' RNA
                     polymerase activity [Evidence IEA];
                     GO_process: GO:0006351 - DNA-templated transcription
                     [Evidence IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="DNA-directed RNA polymerase subunit alpha
                     C-terminal domain-containing protein"
                     /protein_id="HOQ53391.1"
                     /translation="SPVDEQLAADLALPVEELKLTMRSYNCLKREGIHTVSELVSRSE
                     QDLLDIRNFGSKSIEEVKLRLHEMGLSLKDSSPGFDPLAALSRYNEEDDDADFGDDY"
BASE COUNT          731 a         1782 c         1815 g          822 t
ORIGIN      
        1 gtaggagccg aggccggggc cctggccggc gccgatcgcg gaacgtccgc gcaggtcggg
       61 caggccgaag gtggtctgtc cgttgccgcc gtacatggtg ccgagcagcg agaacagtgc
      121 cgtgtactgc tgaatcggca ggagctggcc gttgcactgc gcccaatcat tgggtgcgaa
      181 gttgaagccg aaacagcgca cttcgccgat gtacgcgtcg gtcatggggg tctccttgcg
      241 tgaggtgggt acccggccct cgtcggccgg gcggcaacag tctgctccgg cagagggcgt
      301 cgcgccagac tcgtgcgcga caggtggacg gcgggcgccc gatcgaaccg ccgacggcga
      361 ccgagcccgg gtggctcggg tcaccctcgc tcgattggtc gccgctaggc tggaggcccc
      421 gagcgcggtg cgtcaccgac ctccgccacg tccggatcaa ggtgaagatg aagaagcgga
      481 tcggcatcat cgatgaccac ccggccgtga tgctcggggt ggcctcgatt ctcaacgcac
      541 agcccgacgt gcaggtcgtc gtcaccgcgc tcaccgcggc cgaactggtc gccacccgac
      601 aacccctgga cggggtgttg ctcgacctgc ggctggccga ccgttcgacc cccaccgaca
      661 acctgcgaac cttggccgcc ctcggcgcgc ccgtgctggt ctacacctcg ggcgagcagc
      721 cggagttgat tcgcgaggcg gcccgcgccg gcgtggccgg catggtgcgc aagtcggagt
      781 tgccgcaggt gatggtcgaa gccgtccgcg ccatgctgcg cggcgaggtg gtcgcctccg
      841 cagactgggc cgcggcactc gatgtcgacc cggagttcgt ccgcgtgagc ctgaccgccc
      901 gtgaggccga ggtgcttgag ctgtacgccg ccggagagac cgccgaacgg gtcgccgagg
      961 cactcttcat ctcgcgtgag acggtgctcg accacgtgcg ccgcattcgg gccaagtacg
     1021 cggcggcgga tcgtccggcc cccaccaagg tcgacctcta ccggcgggcc gtcgaagacg
     1081 gtcacctcgt cccgcgccat gaccgtctcg gctgacgggc cgctcgcccc gttcgatcgc
     1141 gggccgagcg aggtggagcg ctccctgggc tggctgatcg ccgtgggcgg ctgcattctg
     1201 ctgctcgacc agaccgtgtt caaggtgggc cacgcgcagc acttcgccgg ctggtggaac
     1261 gccgccggcg ttctggtggc cggcacgctg atcctgctgg cgatcgccgg acgccacctg
     1321 cccggccgcg tgctgcgcgt gctgtgggcc gccgtgccgc tcggctacgc catgctgcag
     1381 gccggctgga gcctggccta cgccggcacc gacccgaacg cggcgatgcc gtggctgtgg
     1441 accctcgaac cggccgcgct gaccctgctg ctgctcgtgc tgaccccgcc ctccgccatc
     1501 gcggttgcgc tggcgatccc cctgctgcct gccctgtcgg ggtggctgcg gttcggtgag
     1561 gtgccgaccg cgatcctgca cgagacgccc agccagctcg gcaacgtggt gtacctggcg
     1621 ctcttcatcg gcctcagcga gcagctgaag cgactgagcg ccagcgaggc agaggcccgc
     1681 cgtcagttgc agcggcagca tcgaaccacc gcccgcaagc gccaacagga cgccctgcag
     1741 cgtctcgtgc acgacgaggt gctgtcggtg ctggcgacgg cgatgcacgc cgacggcgca
     1801 ccctcgaccg ccctgtgcca ggacgccgcc cgggctgcgg tcgcactgga gcggtcgctg
     1861 gagtcgccgg gcggtggttc ggtgcctgcc gatgccgcgg tggatcaggt ggtgtcacag
     1921 ctgcgccgca tcgaccccgg cctggcgctt cacacccagc gcggcgacgg cgactttccg
     1981 gccgacgcgg tgcaggccct caccaaggcg gccgccgagg cgctgcgcaa cagcctggcg
     2041 cacgcgggcc cgtccagcac ccgcagggta tgcgtcacag tcgaccgggg cgaactcgtc
     2101 gtcgtggttc gcgacgacgg aattggcttc gatccggacc cggcctgccc gcggctcggc
     2161 atcgccggca gcatcgtcgg ccgcatgcgc gacgtcggcg gctcggcgcg ggtcgactcg
     2221 aagcccacgc gcggcaccga ggtggtgttg gcatggccga cctgacctcg gcgggcaccg
     2281 gggcaggtgg gttggggacg ccggaggctc gctggtcctt catcttcctg ctctgctgcg
     2341 gggtcgcggg tattgcgctc ggcggccagt gggcgcaggc acccctgctg tggaccctcg
     2401 cggggctggc cgcactggcc gcctctctgc tgctgaccac gcccgtacgc catccgctgc
     2461 cgccgtcgcg cgtggtctgg atcccaccgc tggcgttgct ggtcgcggcc gtcgccctgc
     2521 accagacgga gcctcacaac gacttcgcgg tgctcagctt cgcgtcgtac ctggtcacgt
     2581 tcctgatcgt ccgcggcaac gtgctggccg gcggcctggg cagcgctctg gtgatcgggt
     2641 acgccctggc ctgggtgctg ccacagcaac ccgatctgcg catgctcggc gaggtgctgg
     2701 gcgttcccct gggcggggtg gccgccgcgg tggcgtggcg gctcgtgctg cgggccatcg
     2761 tccggcagga gcgggcccac cgtggcgagg cggcgatggc ggccgaccgg gcccggtggt
     2821 cggcgctcgc ggtgcggcgc tccgaggccg aactcaccac gatccgcacc gtggtcgtgc
     2881 cgttgctgaa ccgggtcgcg gccggcgaac ccatcgatgc gaccctgcgc gccgaactgg
     2941 cgcgcacgga ggcgtccgtg cgtgaccgca tccgggtgcc ccaactgcag catccgcagt
     3001 tggtcgccac catcgaggcc ctgcggctgc gtggggtcgg tgtgctggtg ctgggcgagc
     3061 cgcaactcgg tgagccgatc ggcgaggcgc tggcacttcg gctgcacgcc gaactcgaac
     3121 cgctccagac cggacgtgtc accgtgcggg ccctgcccga tgaccgtccg gccgcgatca
     3181 gcgtgttgat tcaggacgag gccggctccc ggcgtttgca gttctcggca tcgggcgtgc
     3241 tgttgtcgaa gggcgagacc gtactcattc gatgaaggcg agcagcagtc cgaccagtat
     3301 gaacaggctg ccgaagatgc ggctgaccag gcgctggccg gccggatcgc gcatgaaccg
     3361 acgcaacgcc ttcgcggcga gcgcgaagaa gaaccacatc acgatgatgt cgatcgttac
     3421 cagggtggcg gcatagatca ggtattgcgt cagcaggtca ccgtccagcc gaatgaactg
     3481 cggcgtgaac gccaaaaaga acacgatcgc ctttgggttg gtcaggttca ccagcacgcc
     3541 gcgccgaaac atcggcccgg gctgcaggta cgtgggccgt tcggcgtcct gcgcgacctc
     3601 ggcgtccaac tggccgacgc ccgtcggtcg atccagccag taccgcacgc cgagaaacac
     3661 cagataggcg gcgccgagcc aacggatcgc ggtgaacagc cagtggttcg cggcgaccag
     3721 gaccccgacg ccgagcgcga cgatcaggat gtgcaccagc agcgccacct gttgccccag
     3781 tacaccccag atcgagcgcg cccagcctcc ggccagcgag ttcgccatgg tgctgatggc
     3841 gcccgcgccg ggagtgaacg agatcagcag tccggcaccg accaacgtcc accacaacga
     3901 cacctgcacc ggcccagact aggggtctct tcgagcgctc gcctaagtat ctctggcctg
     3961 cgaagctacg cgggccaggg atagttaggc gggggttcgg acgcactgat gcccggcccc
     4021 ccatgaggga gccgggcatc agaacacgga cgatcaggcc tcgtcggcat ccttcaccgc
     4081 gttgacgaag ccggccttct cggcggcctc ggcggagtcg aaccacacct cggcgacggt
     4141 cgaggcgtac cacggcgact cgggggtgtg gaacttcatc gagtcctcgt tgcccttgat
     4201 gtcgaaaccc tcgggcgggt tctcaccgcg gtaaccaccc tcgggcgcgt caccggccag
     4261 gttggacgcg ccgacgctca cctcgccgat cgcgtcgtcg gcagaaggtg cggacggcgc
     4321 cgaaggtgca gacggcgacg acgggctgga cggagcggac tccgccttct tggccctcct
     4381 cggggcggcc ggcttgtcgg ccttcttggc cggggcggcg gacgaaccct tgcccttggc
     4441 cttgttctta cgcgactcct cgacggactc ggtgatcacc tcgatcaccg ccatcggggc
     4501 gttgtcgccc ttgcgcgggc cgatcttggt gatgcgggtg tagccgccgt cacgatcggc
     4561 cagcttcggc gcgatctcgg tgaacagcac gtgcaccaca cccttgtcgg tgatggtcga
     4621 gagcacctgg cgacggttgt gcaggtcgcc ccgcttggcc ttcgcgatca gccgctcggc
     4681 cagcggctga acccgcttgg ccttggcctc ggtggtggtg atccggccgt gctcgaagag
     4741 ctggctggcc aggttggcca gaatgatccg ctggtgtgcc ggcgagcccc cgaaccgggg
     4801 acccttggtt ggcttgggca tgactcgata ctcctattcg cggatcagta gtcgtcgccg
     4861 aagtcggcgt cgtcgtcttc ttcgttgtaa cggctgaggg cggccagcgg gtcgaaaccg
     4921 ggtgagctgt ccttcagcga caggcccatc tcgtgcagcc gcagcttgac ctcttcgatc
     4981 gacttggagc cgaagttgcg aatgtcgagc aggtcctgct ccgagcgcga gaccagctcc
     5041 gagacggtgt ggatgccctc acgcttgagg cagttgtagc tgcgcatggt cagcttgagc
     5101 tcctccaccg gcagcgccag gtcggcggcc agctgctcgt ccaccggcga
//
LOCUS       DAOSUG010000185         5126 bp    DNA     linear   ENV 10-JUN-2024
DEFINITION  MAG TPA_asm: Micropruina sp. isolate AMR_MDS_2195
            NODE_7210_length_5126_cov_4.866693, whole genome shotgun sequence.
ACCESSION   DAOSUG010000185 DAOSUG010000000
VERSION     DAOSUG010000185.1
DBLINK      BioProject: PRJNA925477
            BioSample: SAMN32864262
            Sequence Read Archive: SRR4236673
KEYWORDS    WGS; ENV; Metagenome Assembled Genome; MAG; Third Party Data; TPA;
            TPA:assembly.
SOURCE      Micropruina sp. (wastewater metagenome)
  ORGANISM  Micropruina sp.
            Bacteria; Bacillati; Actinomycetota; Actinomycetes;
            Propionibacteriales; Nocardioidaceae; Micropruina.
REFERENCE   1  (bases 1 to 5126)
  AUTHORS   Abdulkadir,N., Saraiva,J.P., Zhang,J., Stolte,S., Gillor,O.,
            Harms,H. and Rocha,U.
  TITLE     Genome-centric analyses of 165 metagenomes show that mobile genetic
            elements are crucial for the transmission of antimicrobial
            resistance genes to pathogens in activated sludge and wastewater
  JOURNAL   Microbiol Spectr 12 (3), e0291823 (2024)
   PUBMED   38289113
REFERENCE   2  (bases 1 to 5126)
  AUTHORS   Abdulkadir,N.
  TITLE     Direct Submission
  JOURNAL   Submitted (24-MAR-2023) Environmental Microbiology, Helmholtz
            Center for Environmental Research -UFZ, Permoserstr 15, Leipzig
            04318, Germany
COMMENT     The annotation was added by the NCBI Prokaryotic Genome Annotation
            Pipeline (PGAP). Information about PGAP can be found here:
            https://www.ncbi.nlm.nih.gov/genome/annotation_prok/
            
            ##Genome-Assembly-Data-START##
            Assembly Method        :: SPAdes v. 3.11.1
            Genome Representation  :: Full
            Expected Final Version :: Yes
            Genome Coverage        :: Not Applicable
            Sequencing Technology  :: Illumina MiSeq
            ##Genome-Assembly-Data-END##
            
            ##Genome-Annotation-Data-START##
            Annotation Provider               :: NCBI
            Annotation Date                   :: 05/04/2023 04:57:10
            Annotation Pipeline               :: NCBI Prokaryotic Genome
                                                 Annotation Pipeline (PGAP)
            Annotation Method                 :: Best-placed reference protein
                                                 set; GeneMarkS-2+
            Annotation Software revision      :: 6.5
            Features Annotated                :: Gene; CDS; rRNA; tRNA; ncRNA
            Genes (total)                     :: 2,776
            CDSs (total)                      :: 2,750
            Genes (coding)                    :: 2,714
            CDSs (with protein)               :: 2,714
            Genes (RNA)                       :: 26
            tRNAs                             :: 24
            ncRNAs                            :: 2
            Pseudo Genes (total)              :: 36
            CDSs (without protein)            :: 36
            Pseudo Genes (ambiguous residues) :: 0 of 36
            Pseudo Genes (frameshifted)       :: 2 of 36
            Pseudo Genes (incomplete)         :: 32 of 36
            Pseudo Genes (internal stop)      :: 2 of 36
            ##Genome-Annotation-Data-END##
FEATURES             Location/Qualifiers
     source          1..5126
                     /organism="Micropruina sp."
                     /mol_type="genomic DNA"
                     /submitter_seqid="NODE_7210_length_5126_cov_4.866693"
                     /isolate="AMR_MDS_2195"
                     /isolation_source="Activated sludge of municipal
                     wastewater treatment plants"
                     /db_xref="taxon:2737536"
                     /environmental_sample
                     /geo_loc_name="Canada"
                     /lat_lon="49.25 N 123.1 W"
                     /collection_date="2015-06-16"
                     /metagenome_source="wastewater metagenome"
                     /note="metagenomic"
     gene            172..1236
                     /locus_tag="PLF56_07245"
     CDS             172..1236
                     /locus_tag="PLF56_07245"
                     /inference="COORDINATES: ab initio
                     prediction:GeneMarkS-2+"
                     /note="Derived by automated computational analysis using
                     gene prediction method: GeneMarkS-2+."
                     /codon_start=1
                     /transl_table=11
                     /product="hypothetical protein"
                     /protein_id="HOQ53392.1"
                     /translation="MISQEPEPEQPAANAGASPAAGGEPRPGSDVGAVRRPRRLWIAA
                     AVVAVVAVVGGIAWAVRGQPAGPVPAATPQQAVTGFLQALAEADADRALLYARDQPTD
                     KTLLTREVLDESRKLAPLTIVDVPQVQGGGTVQVPAAVKFGEQSATITFSLTTTEQGW
                     RLGQVTSIIDPGPLPAALDATLNGRELSNPSHLEVFPGVYTFGESLPEISLQGDRVVV
                     AAVGEDIRAGLQPTLTKAGVKAANEIAADAVTACLKKRDPAPEGCPNSVKVSAGQKLD
                     ASTIRWNLVGDPWKNATYTLDVADPTKTRGAAKLTFRFRCTLTQNGEKYMVDQTNSVD
                     VRYLLTVTDPKAAVVWQRIA"
     gene            1268..2089
                     /locus_tag="PLF56_07250"
     CDS             1268..2089
                     /locus_tag="PLF56_07250"
                     /inference="COORDINATES: protein motif:HMM:NF013862.2"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="patatin-like phospholipase family protein"
                     /protein_id="HOQ53393.1"
                     /translation="MLGGGGLTGIAWLSGVLDELPPVLGTADRVVGTSAGAVVAARLL
                     WGGDPSTLGDLVPQLGDARLRPGAMARLLAAQVWPSRRHALQWLGRRAVRPVGQVARL
                     TARSGSPGPTQAALVDLVGRAIGSDAWPPALIVVAVDALAGRPAYFTARSQVPLASAV
                     AASCAMPGVLPAVRIEGRWFLDGGLRSPANADLATGCDRVLVLAPQWRSARALRRPEH
                     QADRLRAHGADVVVLRSANRERQSLSAAALPAAREAGRRQGRAIAAEVGRLWGEG"
     gene            complement(2143..2943)
                     /locus_tag="PLF56_07255"
     CDS             complement(2143..2943)
                     /locus_tag="PLF56_07255"
                     /inference="COORDINATES: protein motif:HMM:NF013718.2"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="phosphatase PAP2 family protein"
                     /protein_id="HOQ53394.1"
                     /translation="MHPLRPSADEKTIGSRDLTRWTSRPGRWLSRAFSALSDRLRNID
                     LAALLLLLVSAVLAVGATMLAGEIYEAVVSGHGVSQLDEPVLQWMVAHRSPGFTAVVA
                     AFSNSGGVLWMTVITGAAVATLCVRWRRWTPLVLMLIAVLPALAMTFFGKRITDRTRP
                     PHWASVPPYETSASFPSGHTLNSIVITGMFCYLLVSHLTSTLARVATIVIGTVYTVSM
                     GLSRVYLGHHWLTDVLAAWCLGIAWLTVVITAHRLWLTKHEDRRLRRA"
     gene            3168..>5126
                     /locus_tag="PLF56_07260"
     CDS             3168..>5126
                     /locus_tag="PLF56_07260"
                     /EC_number="6.4.1.1"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_015068808.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_function: GO:0004736 - pyruvate carboxylase activity
                     [Evidence IEA];
                     GO_function: GO:0005524 - ATP binding [Evidence IEA];
                     GO_function: GO:0009374 - biotin binding [Evidence IEA];
                     GO_function: GO:0046872 - metal ion binding [Evidence
                     IEA];
                     GO_process: GO:0006090 - pyruvate metabolic process
                     [Evidence IEA];
                     GO_process: GO:0006094 - gluconeogenesis [Evidence IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="pyruvate carboxylase"
                     /protein_id="HOQ53395.1"
                     /translation="MFRKILVANRGEIAVRAFRAATELGVGTVAVFPFEDRFAEYRLK
                     ADESYQIGEVGHPVRAYLDVAGIIAAALEAGVDAIYPGYGFLSENPDLAQACEDNGIT
                     FVGPPHTVLELTGDKAAAIAAARAAGLPTLRGSEPSDDPEVLIAAAGEIGFPVFVKAV
                     SGGGGRGMRRVETPEALPAALTQAMREAQAAFGDSRMYLEQAVLGPRHIEVQILADTQ
                     GNVIHLFERDCSVQRRHQKVVELAPAPNLDPALREAMCADAVKFARSIDYCNAGTVEF
                     LLGADGRYVFIEMNPRIQVEHTVTEEVTDVDLVSSQLRIAAGDSLSDLGLSQDSIHVR
                     GQALQCRVTTEDPSNGFRPDTGRLSVYRTPGGAGVRLDGGVVFAGAEVSAHFDSMLVK
                     LTCRGRDLPSAARRAYRALTEFRIRGVSTNIPFLEAVITDPDFLAGRATTSFIEERPQ
                     LMNHRMPADRGTKLLTYLAGVAVNKPNGEARTTLIARDKLPALSRAELSAPAPSGSKQ
                     LLLTEGPERFAQALRERTAVAVTDTTFRDAHQSLLATRVRTRDLLHVAPYIARMLPGL
                     FSMESWGGATYDVTLRFLKEDPWDRLAKFAEAMPNIPQQMLLRGRNTVGYTPYPLAVT
                     RAFVHEAARTGCDIFRIFDALNNIDQIRP"
BASE COUNT          770 a         1780 c         1829 g          747 t
ORIGIN      
        1 ctccacgggt tcggcgaagg tgtaggtggt gcggtgttcg agagctaccc ggatcgtcac
       61 ccctggcaat gtagcggcac ccggcagggc tgagcaccgg ttgaacagca ccgaaacctc
      121 actgaaactt tgcaggcgcg gaccttgggc ctgcaccact accctggttc agtgatttct
      181 caggaacccg aacccgagca gccggcggcg aacgccgggg cgagccccgc tgccggcggc
      241 gaaccgcgtc cgggttcgga cgtgggcgca gtgcgtcggc ctcgccggct gtggatcgcc
      301 gcagcggtgg tcgccgtggt ggccgtggtg ggcggcatcg cgtgggcggt gcgcggccag
      361 ccggccggtc cggtacccgc ggccaccccg cagcaggcgg tgaccggctt cttgcaggcg
      421 ttggccgagg ctgatgccga ccgcgccctg ctgtatgccc gcgaccagcc gaccgacaag
      481 accctgctga cccgtgaggt gctggacgaa tcccgcaagc tggccccgtt gaccatcgtg
      541 gacgtgccgc aggtgcaggg cggcggcacg gtgcaggtgc cggcagcggt gaagttcggc
      601 gagcagagcg cgacgatcac gttctcgctc accaccaccg aacagggctg gcggttgggt
      661 caggtcacct cgatcatcga ccccggaccg ttgccggcgg cgctggatgc caccctgaac
      721 gggcgcgaac tcagcaatcc ttcgcacctc gaggtgtttc cgggcgtcta caccttcggc
      781 gagagcctgc ccgagatcag cctgcagggc gaccgcgtgg tggtcgcagc cgtcggcgag
      841 gacattcgcg cgggcctgca gccgaccctg accaaggccg gggtgaaggc ggccaacgag
      901 atcgcggccg acgccgtcac cgcctgcctg aagaagcgcg acccggcacc ggagggctgc
      961 ccgaacagcg tcaaggtgtc agccgggcag aagctcgacg cctcgaccat tcggtggaac
     1021 ctggtcggcg acccgtggaa gaacgccacc tacaccctgg acgtcgccga tccgaccaag
     1081 acgcggggcg ccgcgaaact caccttccgg ttccgctgca cgctgacgca gaacggtgag
     1141 aagtacatgg tcgaccagac caactcggtc gacgtgcgtt acctgctgac ggtcaccgat
     1201 cccaaggccg cggttgtctg gcagcggatc gcctgaaccg ttcggcggcc ccggcggcag
     1261 ggcggtcgtg ctgggcggcg gtgggctcac cggcatcgcg tggctgagcg gtgtgctcga
     1321 tgagctgccg ccggtgctcg gcacggccga ccgggtggtc ggtacgtctg cgggtgcggt
     1381 ggtggcggca cgactgttgt ggggtggtga cccgtccaca ctcggcgacc tcgttccgca
     1441 gctcggcgat gcgcggctcc gtccgggtgc catggcgaga ctgttggcgg cgcaggtgtg
     1501 gcccagccgc cggcatgcgt tgcagtggct cggacgacgc gcggtgcgtc cggtggggca
     1561 ggtggctcgc ctcacggcga ggtccggctc gcccgggccg acgcaggctg ccctcgtcga
     1621 cctggtgggg cgcgccatcg gttcggacgc ctggccgccg gcgctgatcg tggtggcggt
     1681 cgatgccctg gccggacgtc cggcctactt caccgcgcgc tcgcaggtgc cgctggcatc
     1741 ggcggtcgcg gccagttgcg cgatgccggg cgtgctgccc gccgtccgga tcgagggtcg
     1801 gtggtttctg gacggtggcc tgcgctcgcc ggccaacgcc gacctggcca cgggctgcga
     1861 ccgggtgctg gtgctggcgc cgcagtggcg ctcggcgcgg gctctgcggc gaccggagca
     1921 tcaggccgac cggttgcggg cccacggggc cgacgtggtg gtgctgcggt cggcgaaccg
     1981 ggaacggcag tcactgtcgg cggcggcgct gccggcggcc cgcgaggccg gacggcgcca
     2041 gggtcgggcg atcgcggcgg aggtcggccg gttgtggggt gaaggctgag caggaggtgg
     2101 ggagagggcc gagcagaagg tggggccggt tcccagtcct gctcaggctc ggcgcagcct
     2161 tcggtcctcg tgcttggtca accacagccg gtgcgccgtg atcaccacgg tcagccaggc
     2221 gatgccgagg caccaggcgg cgagcacgtc ggtcaaccag tgatgaccca gatagacccg
     2281 cgacaggccc atcgacacgg tgtacaccgt gccgatcacg atcgtggcga cccgggccag
     2341 cgtgctggtc aggtgcgaga cgagcaggta gcagaacatg ccggtgatca cgatcgagtt
     2401 cagggtgtgc ccggacggga acgacgccga ggtctcgtac ggcggcacgg acgcccagtg
     2461 cggcggacgg gtgcgatcgg tgatccgctt gccgaagaac gtcatggcca gtgcgggcag
     2521 caccgcgatc agcatcagca ccaggggcgt ccaacgtcgc caacgcacgc acagggtggc
     2581 gaccgcggcg ccggtgatga ccgtcatcca cagcacgccg cccgagttgc tgaacgcggc
     2641 caccacagcg gtgaagcccg gggagcggtg ggccaccatc cactgcagca ccggctcgtc
     2701 caactggctg acgccgtgac cggacaccac ggcttcatag atttcaccgg ccagcatggt
     2761 ggcgcccacg gccagcaccg ccgagaccag cagcagcagc agggccgcca ggtcgatgtt
     2821 gcgcagccgg tcggacagcg cgctgaaggc ccggctcagc cagcggccgg gtcggctcgt
     2881 ccaccgggtc aggtcacgtg agccgatggt cttttcatca gcggacggac gcagggggtg
     2941 catcggcaca gtgtgccagg cgagcctgcg gccaggtcgt tggccaaccc gcgcgtcgcc
     3001 tcgtcgcccg cgtcgtcgtc cgcgtcgtcg tcccttcgcc aacggccgga tcccgcaacc
     3061 ggatcgaacg cgttcccaca ccggggggtt tgtgtcaggc tttcggccag acggcggttg
     3121 agcgatgcgt tcgccgggtg cgtccgacag tgtggtgaag gagatccatg ttccgcaaga
     3181 ttctggtcgc caaccgcggc gagatcgccg tgcgtgcctt tcgcgcggcg accgaactgg
     3241 gggtcggcac cgtggccgtc tttccgttcg aggaccgctt cgccgagtac cggctgaagg
     3301 ccgacgaaag ctaccagatc ggcgaggtcg gacacccggt gcgcgcctac ctcgacgtgg
     3361 caggcatcat cgccgccgcg ctcgaagccg gcgtcgacgc catctatccc ggctacggct
     3421 tcctttcgga gaaccccgac ctggcgcagg cctgcgaaga caacggcatc acgttcgtcg
     3481 ggccgccgca caccgtgctc gaactgaccg gcgacaaggc cgccgccatc gccgccgcgc
     3541 gtgccgccgg gctgcccacg ttgcgcggca gcgagccgtc cgacgacccg gaggtgctga
     3601 tcgccgctgc cggtgagatc ggctttccgg tgttcgtcaa ggccgtctcg ggcggtggcg
     3661 gacgaggcat gcgccgggtc gagacccctg aggcgctccc cgcggcgctc acccaggcga
     3721 tgcgcgaggc gcaggcggcc ttcggcgaca gtcggatgta cctggagcag gccgtgctgg
     3781 gcccgcggca catcgaggtg cagattctgg ccgacaccca gggcaacgtg atccacctgt
     3841 tcgaacgcga ctgctcggtg cagcgccggc accagaaggt ggtcgagttg gcgcccgcac
     3901 cgaacctcga cccggcgctg cgcgaagcga tgtgcgccga cgcggtgaag ttcgcccgca
     3961 gcatcgacta ctgcaacgcc ggcacggtcg agtttctgct cggcgccgac ggacgctacg
     4021 tgttcatcga gatgaacccc cgcatccagg tcgagcacac ggtcaccgaa gaggtcaccg
     4081 acgtcgacct ggtcagctcg cagttgcgga tcgccgccgg cgacagcctg tccgacctgg
     4141 ggctgagcca ggactccatt cacgtccgcg gccaggcgct gcagtgccgg gtgaccaccg
     4201 aggatccgtc caacggcttc cggcccgaca ccggacgcct gtcggtctac cgcacgcccg
     4261 gtggtgcggg ggtgcggctg gacggtggcg tggtcttcgc cggcgccgag gtcagcgccc
     4321 acttcgactc gatgctggtc aagctgacct gtcgcggccg cgaccttcct tcggcggccc
     4381 ggcgggccta ccgtgcgctc accgagttcc gcatccgcgg cgtctcgacg aacattccgt
     4441 tcctcgaggc cgtcatcacc gaccccgact ttctggccgg ccgggcgacc acatcgttca
     4501 tcgaggaacg tccgcaactg atgaaccacc ggatgcccgc cgaccggggc acgaagctgc
     4561 tcacctacct ggccggcgtc gcggtgaaca agcccaacgg cgaggcccgc accacgctga
     4621 tcgcccgcga caagctgccc gccctgagcc gggccgaact gagcgcgcca gctccgtccg
     4681 gatcgaagca gctgctgctc accgagggcc cggaacggtt cgcgcaggca ctgcgcgagc
     4741 ggaccgcggt cgccgtgacc gacaccacct tccgggacgc gcaccagagc ctgctggcca
     4801 cccgggtgcg cacccgcgac ctgctgcacg tcgcccccta catcgcgcgg atgctgcccg
     4861 gcctgttctc gatggagtcg tggggcggtg ccacctacga cgtgacgctg cggtttctga
     4921 aggaggaccc ctgggatcgc ctcgccaagt tcgccgaagc catgcccaac attccccagc
     4981 agatgctgct gcgcggacgc aacaccgtcg gctacacccc ctacccgctg gcggtgaccc
     5041 gggccttcgt gcacgaggcg gcgcgcaccg gctgcgacat cttccgcatc ttcgacgcgc
     5101 tgaacaacat cgaccagatc cggccg
//
LOCUS       DAOSUG010000186         5108 bp    DNA     linear   ENV 10-JUN-2024
DEFINITION  MAG TPA_asm: Micropruina sp. isolate AMR_MDS_2195
            NODE_7234_length_5108_cov_4.517910, whole genome shotgun sequence.
ACCESSION   DAOSUG010000186 DAOSUG010000000
VERSION     DAOSUG010000186.1
DBLINK      BioProject: PRJNA925477
            BioSample: SAMN32864262
            Sequence Read Archive: SRR4236673
KEYWORDS    WGS; ENV; Metagenome Assembled Genome; MAG; Third Party Data; TPA;
            TPA:assembly.
SOURCE      Micropruina sp. (wastewater metagenome)
  ORGANISM  Micropruina sp.
            Bacteria; Bacillati; Actinomycetota; Actinomycetes;
            Propionibacteriales; Nocardioidaceae; Micropruina.
REFERENCE   1  (bases 1 to 5108)
  AUTHORS   Abdulkadir,N., Saraiva,J.P., Zhang,J., Stolte,S., Gillor,O.,
            Harms,H. and Rocha,U.
  TITLE     Genome-centric analyses of 165 metagenomes show that mobile genetic
            elements are crucial for the transmission of antimicrobial
            resistance genes to pathogens in activated sludge and wastewater
  JOURNAL   Microbiol Spectr 12 (3), e0291823 (2024)
   PUBMED   38289113
REFERENCE   2  (bases 1 to 5108)
  AUTHORS   Abdulkadir,N.
  TITLE     Direct Submission
  JOURNAL   Submitted (24-MAR-2023) Environmental Microbiology, Helmholtz
            Center for Environmental Research -UFZ, Permoserstr 15, Leipzig
            04318, Germany
COMMENT     The annotation was added by the NCBI Prokaryotic Genome Annotation
            Pipeline (PGAP). Information about PGAP can be found here:
            https://www.ncbi.nlm.nih.gov/genome/annotation_prok/
            
            ##Genome-Assembly-Data-START##
            Assembly Method        :: SPAdes v. 3.11.1
            Genome Representation  :: Full
            Expected Final Version :: Yes
            Genome Coverage        :: Not Applicable
            Sequencing Technology  :: Illumina MiSeq
            ##Genome-Assembly-Data-END##
            
            ##Genome-Annotation-Data-START##
            Annotation Provider               :: NCBI
            Annotation Date                   :: 05/04/2023 04:57:10
            Annotation Pipeline               :: NCBI Prokaryotic Genome
                                                 Annotation Pipeline (PGAP)
            Annotation Method                 :: Best-placed reference protein
                                                 set; GeneMarkS-2+
            Annotation Software revision      :: 6.5
            Features Annotated                :: Gene; CDS; rRNA; tRNA; ncRNA
            Genes (total)                     :: 2,776
            CDSs (total)                      :: 2,750
            Genes (coding)                    :: 2,714
            CDSs (with protein)               :: 2,714
            Genes (RNA)                       :: 26
            tRNAs                             :: 24
            ncRNAs                            :: 2
            Pseudo Genes (total)              :: 36
            CDSs (without protein)            :: 36
            Pseudo Genes (ambiguous residues) :: 0 of 36
            Pseudo Genes (frameshifted)       :: 2 of 36
            Pseudo Genes (incomplete)         :: 32 of 36
            Pseudo Genes (internal stop)      :: 2 of 36
            ##Genome-Annotation-Data-END##
FEATURES             Location/Qualifiers
     source          1..5108
                     /organism="Micropruina sp."
                     /mol_type="genomic DNA"
                     /submitter_seqid="NODE_7234_length_5108_cov_4.517910"
                     /isolate="AMR_MDS_2195"
                     /isolation_source="Activated sludge of municipal
                     wastewater treatment plants"
                     /db_xref="taxon:2737536"
                     /environmental_sample
                     /geo_loc_name="Canada"
                     /lat_lon="49.25 N 123.1 W"
                     /collection_date="2015-06-16"
                     /metagenome_source="wastewater metagenome"
                     /note="metagenomic"
     gene            <1..668
                     /locus_tag="PLF56_07265"
     CDS             <1..668
                     /locus_tag="PLF56_07265"
                     /inference="COORDINATES: protein motif:HMM:NF012711.2"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=3
                     /transl_table=11
                     /product="AMP-binding protein"
                     /protein_id="HOQ53396.1"
                     /translation="ERWETATGGLVVEGYGMTETSPVALGNPFDASRRPGSLGLPFPG
                     TEVRVVSLTDPEQTVPRGEPGELQIRGPQVFLGYWNRPDETAHQLSADGWLRTGDVVQ
                     IDDDGFVTLVDRIKEMIVTGGHKVYPSQVEDWLRTMPGVTDVAVVGVPGGDMGERVVA
                     ALTVTDGHHIDLDAVREWCRPRLAKYAIPRQVVLLKELPRSQVGKVLRRVVREQVLDR
                     RHK"
     gene            complement(696..1322)
                     /locus_tag="PLF56_07270"
     CDS             complement(696..1322)
                     /locus_tag="PLF56_07270"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_013865274.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_function: GO:0003677 - DNA binding [Evidence IEA];
                     GO_function: GO:0003700 - DNA-binding transcription factor
                     activity [Evidence IEA];
                     GO_process: GO:0006355 - regulation of DNA-templated
                     transcription [Evidence IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="TetR/AcrR family transcriptional regulator"
                     /protein_id="HOQ53397.1"
                     /translation="MPRIEAPTVAEHREMIRTRLINAAEQLLRATPSAPLSAGAVTAQ
                     AGIARNSIYRYVDSVEDLRGLVVNRYLPDWLGAVAEAMQHVDRPENRVVTWVRANLEQ
                     AAGTGHGWLMEAARHQAPNASIDETVNQAHAGMRSSLSDAWSELVASHPERLPIAVGF
                     TVGILDAGFRQLDQGRPAELVADLGTTAARALVHGLRSEPDRPLATRD"
     gene            1516..1977
                     /locus_tag="PLF56_07275"
     CDS             1516..1977
                     /locus_tag="PLF56_07275"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_013865275.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="DUF2871 domain-containing protein"
                     /protein_id="HOQ53398.1"
                     /translation="MKNAETVLFRLLVTWTTLGLASGLAYRELTRSVGFSGYTQLSLV
                     HTHTLVLGTVVGMILLVMERLYRLSDDRRFVWFVWVWNIGLGLTAGTLAVKGTLQVLS
                     SPAAGSAALAGIAGLGHITLTAGFVLMLLVLGRRIRLSRPALIEQAGGGLQ"
     gene            1974..3533
                     /locus_tag="PLF56_07280"
     CDS             1974..3533
                     /locus_tag="PLF56_07280"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_013865276.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="MFS transporter"
                     /protein_id="HOQ53399.1"
                     /translation="MTSIPASPAVAEPSAGPRAWKGLWVLASALAMIVLDGTIVAVAL
                     PSIIADLRLDLTDAQWVNSLYSVVFAALLLTAGRLGDRWGRRRLLLIGLTVFVAGNVL
                     AALAVSVTPLLMARAVQGIGGAMVLPSTLSSVNATFRGPDRAAAFGVWGAVMAGAAAL
                     GPLVGGVLTQFASWPLIFWVNVPIGIAVIVGTLRLVDETSADETAGGVDWAGPLLSAA
                     GLGLIVFGLIESSSLGWWTQTEPLRLFGFTWPPTAPISPIPVALAAGAMLLSGFVLVE
                     SRRQRTGRATVLDLRLFRLTTFSLGNGTALLVAVGEFALLFVLPLFLVTSLALSVLAA
                     GAVLASMATGAFLSGAAARHLAARFGATRVVVLGLVLELLGAGATALAVAASASAWTL
                     VATLLPYGLGLGLASAQLTSTTLRNVPSAQSGSASATQSTVRQLGAALGTALGGTALA
                     TALGSGSATTTLADPVTFARASGAALWVAVGILAVATLTAWGLDRASGRPENRQPHQP
                     TQEAPCSFQPS"
     gene            3512..3901
                     /locus_tag="PLF56_07285"
     CDS             3512..3901
                     /locus_tag="PLF56_07285"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_013865277.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="HsmA family protein"
                     /protein_id="HOQ53400.1"
                     /translation="MLVPAIVIITLALVFYTVGVWGERVQGTLKPWHAGMFALGLACD
                     ATGTWLMTQIAAERRGEGIQPGALSAWMAVSGTIAIILMAVHLMWAVVVLVRNREREK
                     LTFHTFSLAVWVVWLASYIIGAASAMV"
     gene            complement(3929..>5108)
                     /locus_tag="PLF56_07290"
     CDS             complement(3929..>5108)
                     /locus_tag="PLF56_07290"
                     /inference="COORDINATES: ab initio
                     prediction:GeneMarkS-2+"
                     /note="Derived by automated computational analysis using
                     gene prediction method: GeneMarkS-2+."
                     /codon_start=2
                     /transl_table=11
                     /product="hypothetical protein"
                     /protein_id="HOQ53401.1"
                     /translation="SIVVNNLDETWSMLTPATVTTAALPGDAPACPTGMTPISWIAYD
                     TGGVLLCRAGTNYAVVYSTHPDWSATRLSFSAGGYQVEFSNQAVLQASLGGALLSLAQ
                     DGTVSAYPATAVWNSEAGVTAFAIPDGLPSCPIGSWPISLSSFKGGWLLVCGVAGDRP
                     TSMVFAHNGEVTDTATVTRQTTGYCGTFGTTTVCGYPVPGVVTEQDAGGSTQQYSATS
                     NYFAGYGQNKAGEGNGAFGLRVPDRTAADQVRYLTQLVQKSMIGRSNLNLAVDQVRGC
                     TNLQGAVDSIGTVVTNRQTLLSALDTAPVDFVPNGDTLLAELRTSIQLSYESDVLWLQ
                     WAESERDTHCAQGVHHPLYTQIGELNKGVATAKYAFVNDWNATIAPTFSAPRFTRAQI
                     "
BASE COUNT          730 a         1735 c         1765 g          878 t
ORIGIN      
        1 ccgagcgctg ggagacggcc accggcggcc tggtcgtcga gggctacggc atgaccgaaa
       61 cctcaccggt cgcgctgggc aacccgttcg atgccagccg ccgtcctggg tccctcggcc
      121 tgccgttccc gggcaccgag gtgcgcgtgg tcagcctgac cgaccccgag cagaccgtcc
      181 cacgcggcga gccgggtgag ctgcagatca ggggcccgca ggtgttcctg gggtactgga
      241 accgtccgga cgagaccgcc caccagctca gcgccgacgg ctggctgcgc accggcgacg
      301 tggtgcagat cgacgacgac gggttcgtca cgctggtcga ccggatcaaa gagatgatcg
      361 tcaccggcgg tcacaaggtc tacccgtcgc aggtcgagga ctggctgcgc accatgcccg
      421 gcgtgaccga cgtggccgtg gtcggcgtac ccggcggcga catgggcgag cgcgtggtgg
      481 cggccctgac ggtgacggac ggccatcaca tcgacctgga cgccgtgcgc gaatggtgcc
      541 ggccgcggct ggctaagtac gcgatcccgc gtcaggtggt actactgaaa gaactaccca
      601 ggtcccaggt cggcaaggtg ctgcgccggg tggtccgcga acaagtgctg gaccggcgcc
      661 acaagtgacg gaagtcggcg ctcagccgct ccgggtcagt cccgcgtggc cagcggtcgg
      721 tccggctcag atcgaaggcc atggaccagc gcccgcgccg cagtggtgcc gagatcggcg
      781 accaactctg caggccgacc ctgatcgagt tggcggaacc ccgcgtccag aatgccgacg
      841 gtgaatccca cggcgatcgg cagccgctcc ggatgggacg ccaccagttc gctccaggcg
      901 tcggagaggc tgctgcgcat gccggcgtgg gcctggttca ccgtctcgtc gatcgacgcg
      961 ttgggcgcct ggtggcgggc cgcctccatc agccagccgt ggccggtgcc cgcagcctgc
     1021 tcaaggttgg cccgcaccca ggtgacgacc cggttctcag ggcggtctac gtgttgcatc
     1081 gcctccgcca cagcgcccag ccagtcaggt aggtagcggt tcacgaccag gccgcgcagg
     1141 tcctccaccg agtcgacgta gcggtagatg ctgttgcgcg cgatgccggc ttgcgcggtg
     1201 acggcgccgg cactcagcgg tgcggacggg gtcgcgcgca gcagctgctc ggcggcgttg
     1261 atcagcctgg tgcggatcat ttctcgatgc tcggcgaccg tgggcgcctc gattcgcggc
     1321 acagctcccc cttcttggct acggcctgcc atggctggct cgaccgcgag ctctctggct
     1381 cgactagctc acccgcaccg gagtgccggc cccaagagcc gccacctccg acagtgaaca
     1441 ccagttttgc ggacacgccg tccctaagct agcctgatgc ttagagacgt attgtcgcaa
     1501 actttggagg atgttgtgaa gaacgcggaa acggtgctgt tccggttgct ggtgacgtgg
     1561 accaccctcg gtctcgccag cggattggcc taccgcgagc tgacccgttc agtcgggttc
     1621 agcggctaca cccagttgtc cctggtgcac acgcacaccc tggtgctggg caccgtggtc
     1681 gggatgattc tgctcgtgat ggagcggctc taccgcttga gcgacgaccg ccgcttcgtc
     1741 tggttcgtct gggtgtggaa catcggcctg gggctgacgg cgggcaccct ggcggtgaag
     1801 ggaacgctgc aggtcctgag ctcgcctgcc gccggctcag cagccctggc cggcatcgcc
     1861 ggactcggtc acatcacgtt gacggccggt ttcgtgttga tgctcctggt gctgggccgt
     1921 cggatcaggc tgagccgccc ggcgctgatc gagcaggcag gaggcgggct gcaatgacat
     1981 cgattcccgc atcacccgcc gtggccgagc cgagcgccgg tccccgcgcc tggaaaggac
     2041 tctgggtgct ggcctccgcc ctggccatga tcgttctgga cggcaccatc gtcgcggttg
     2101 cactgccttc catcatcgcc gaccttcggc ttgacctgac cgatgcccaa tgggtcaaca
     2161 gcctgtactc ggtggtgttc gcggccctgc tgctgacggc tggacgactg ggcgaccgat
     2221 ggggccggcg acgtcttctg ctgatcgggc tgaccgtttt cgtcgcaggc aatgtgctcg
     2281 cagccctggc cgtcagcgtc acacccttgc tgatggcgcg cgccgtgcag ggtatcggcg
     2341 gcgccatggt gctgccgtcc accctctcgt cggtgaacgc gacgttccgt gggccggacc
     2401 gtgcggcagc gttcggagtc tggggcgccg tgatggccgg agcggccgca ctggggccgc
     2461 tggtgggcgg cgtgctcacc cagttcgcca gctggccgct gatcttctgg gtgaacgtgc
     2521 cgatcggcat cgccgtgatc gtcggaaccc tccggctggt ggacgagacg tcggccgacg
     2581 agacggctgg cggcgtggac tgggcgggtc cgctgctcag cgccgccggc ctcggcctga
     2641 tcgtgttcgg tctcatcgag agcagttcac tgggttggtg gacccagacc gagccgctgc
     2701 ggctgttcgg cttcacctgg ccccccaccg cgccgatctc accgattccg gtggcgctgg
     2761 ccgccggcgc catgcttctg tccggcttcg tcctggtcga gtcccgccgg caacgcaccg
     2821 gtcgcgccac agtgctcgac ctgcggctgt tccggctcac caccttcagc ctgggcaacg
     2881 gcaccgcgct gctcgtggcg gtcggcgagt tcgcactgct cttcgtgctg ccgctgttcc
     2941 tggtcacctc gctggccctg agcgtgctgg ccgccggcgc ggttctggcg tccatggcca
     3001 ccggggcgtt cctgtcgggc gccgcagcac ggcacctcgc cgcccggttc ggggccaccc
     3061 gcgtggtggt gctcggcctg gttctcgaac tgctcggcgc cggggccacc gcgctggcgg
     3121 tagccgcctc ggcatcggcg tggacgttgg tcgccacgct gctgccctac ggcctcgggc
     3181 tgggcctggc ctcggcgcaa ctgacctcca ccacgctgcg caacgttccc tcggctcagt
     3241 cgggcagcgc atccgcgacc cagagcaccg tgcgtcagct cggcgcggcc ctgggcacgg
     3301 cgctcggtgg caccgcgctc gcgacggccc tgggcagcgg cagcgcaacg accacgctgg
     3361 ccgacccggt cacgttcgcc cgcgccagcg gcgccgccct gtgggtggcg gtcggcattc
     3421 tggccgtggc caccctcacc gcctggggac tggatcgagc cagcggccgt cccgaaaacc
     3481 gtcagccgca tcaaccaacg caggaggcac catgctcgtt ccagccatcg tgatcatcac
     3541 cttggccctg gtcttctaca ccgtcggtgt gtggggcgaa cgggtccagg gaactctcaa
     3601 gccgtggcac gccggaatgt tcgcactcgg gctggcctgt gacgccaccg gaacctggct
     3661 gatgacgcag atcgccgccg aacgtcgggg cgaaggaata cagcccggcg cgctgagcgc
     3721 ctggatggcg gtgagcggca cgattgcgat cattctgatg gccgtgcacc tgatgtgggc
     3781 cgtggtggtg ctggtgcgca accgtgaacg cgaaaagttg acgttccaca cgttcagcct
     3841 ggccgtgtgg gtggtgtggc tcgccagcta catcatcggt gccgcctcgg ccatggtgtg
     3901 acgagcaggc gggtgattgc ctcccaggct agatctgcgc ccgggtgaag cgtggtgccg
     3961 agaaggtggg cgcgatggtc gcattccagt cgttcacgaa ggcgtacttg gccgtggcca
     4021 cccccttgtt gagctcgccg atctgcgtgt acagcggatg gtggacgccc tgcgcgcagt
     4081 gggtgtcgcg ctccgactct gcccactgca gccacagcac gtccgattcg taagagagct
     4141 ggatcgaggt acgcagctcc gccaggagcg tgtcaccgtt cggtacgaaa tccaccggcg
     4201 cggtgtcgag cgcgctcagc aatgtctgcc ggttggtcac cacggtgccg atcgagtcga
     4261 ctgccccctg caggttggtg cagccgcgta cctgatcgac ggccagattc aggttcgatc
     4321 ggccgatcat cgacttctgc accaactggg tcaggtagcg cacctgatcg gcggcggttc
     4381 ggtcgggcac tcgcaggccg aatgcgccgt tgccctcacc ggccttgttc tggccgtagc
     4441 cagcgaagta gttcgaggtg gcgctgtact gctgcgtcga acctcctgcg tcttgttcgg
     4501 tgaccacgcc gggtaccggg tagccgcaca cggtggtggt gccgaacgtg ccgcagtagc
     4561 ccgtggtctg cctggtcacc gtcgcggtgt cggtgacctc accgttgtgc gcgaagacca
     4621 tcgatgtcgg acgatccccg gccacaccgc agaccagcaa ccacccgccc ttgaagctcg
     4681 acaacgagat cggccaggat ccgatcgggc aggacggcag accgtccgga atggcgaagg
     4741 cagtcacgcc cgcctcgctg ttccacaccg cggtggccgg gtacgcactg accgtgccgt
     4801 cctgcgcgag agagagcagg gcgcccccca gactcgcctg caacaccgcc tgattgctga
     4861 actccacctg ataaccgccg gcactgaacg acagccgggt cgccgaccag tccggatggg
     4921 tcgagtacac caccgcgtag ttcgtgcccg cacggcacag cagtaccccg ccggtgtcgt
     4981 aggcgatcca cgaaatgggc gtcatgccgg tggggcaggc gggcgcatcg ccgggcaggg
     5041 cggcggtggt gacggtggcc ggagtaagca tcgaccaggt ttcgtccagg ttgttcacca
     5101 cgatgctg
//
LOCUS       DAOSUG010000187         5094 bp    DNA     linear   ENV 10-JUN-2024
DEFINITION  MAG TPA_asm: Micropruina sp. isolate AMR_MDS_2195
            NODE_7262_length_5094_cov_4.039690, whole genome shotgun sequence.
ACCESSION   DAOSUG010000187 DAOSUG010000000
VERSION     DAOSUG010000187.1
DBLINK      BioProject: PRJNA925477
            BioSample: SAMN32864262
            Sequence Read Archive: SRR4236673
KEYWORDS    WGS; ENV; Metagenome Assembled Genome; MAG; Third Party Data; TPA;
            TPA:assembly.
SOURCE      Micropruina sp. (wastewater metagenome)
  ORGANISM  Micropruina sp.
            Bacteria; Bacillati; Actinomycetota; Actinomycetes;
            Propionibacteriales; Nocardioidaceae; Micropruina.
REFERENCE   1  (bases 1 to 5094)
  AUTHORS   Abdulkadir,N., Saraiva,J.P., Zhang,J., Stolte,S., Gillor,O.,
            Harms,H. and Rocha,U.
  TITLE     Genome-centric analyses of 165 metagenomes show that mobile genetic
            elements are crucial for the transmission of antimicrobial
            resistance genes to pathogens in activated sludge and wastewater
  JOURNAL   Microbiol Spectr 12 (3), e0291823 (2024)
   PUBMED   38289113
REFERENCE   2  (bases 1 to 5094)
  AUTHORS   Abdulkadir,N.
  TITLE     Direct Submission
  JOURNAL   Submitted (24-MAR-2023) Environmental Microbiology, Helmholtz
            Center for Environmental Research -UFZ, Permoserstr 15, Leipzig
            04318, Germany
COMMENT     The annotation was added by the NCBI Prokaryotic Genome Annotation
            Pipeline (PGAP). Information about PGAP can be found here:
            https://www.ncbi.nlm.nih.gov/genome/annotation_prok/
            
            ##Genome-Assembly-Data-START##
            Assembly Method        :: SPAdes v. 3.11.1
            Genome Representation  :: Full
            Expected Final Version :: Yes
            Genome Coverage        :: Not Applicable
            Sequencing Technology  :: Illumina MiSeq
            ##Genome-Assembly-Data-END##
            
            ##Genome-Annotation-Data-START##
            Annotation Provider               :: NCBI
            Annotation Date                   :: 05/04/2023 04:57:10
            Annotation Pipeline               :: NCBI Prokaryotic Genome
                                                 Annotation Pipeline (PGAP)
            Annotation Method                 :: Best-placed reference protein
                                                 set; GeneMarkS-2+
            Annotation Software revision      :: 6.5
            Features Annotated                :: Gene; CDS; rRNA; tRNA; ncRNA
            Genes (total)                     :: 2,776
            CDSs (total)                      :: 2,750
            Genes (coding)                    :: 2,714
            CDSs (with protein)               :: 2,714
            Genes (RNA)                       :: 26
            tRNAs                             :: 24
            ncRNAs                            :: 2
            Pseudo Genes (total)              :: 36
            CDSs (without protein)            :: 36
            Pseudo Genes (ambiguous residues) :: 0 of 36
            Pseudo Genes (frameshifted)       :: 2 of 36
            Pseudo Genes (incomplete)         :: 32 of 36
            Pseudo Genes (internal stop)      :: 2 of 36
            ##Genome-Annotation-Data-END##
FEATURES             Location/Qualifiers
     source          1..5094
                     /organism="Micropruina sp."
                     /mol_type="genomic DNA"
                     /submitter_seqid="NODE_7262_length_5094_cov_4.039690"
                     /isolate="AMR_MDS_2195"
                     /isolation_source="Activated sludge of municipal
                     wastewater treatment plants"
                     /db_xref="taxon:2737536"
                     /environmental_sample
                     /geo_loc_name="Canada"
                     /lat_lon="49.25 N 123.1 W"
                     /collection_date="2015-06-16"
                     /metagenome_source="wastewater metagenome"
                     /note="metagenomic"
     gene            29..805
                     /gene="hisF"
                     /locus_tag="PLF56_07295"
     CDS             29..805
                     /gene="hisF"
                     /locus_tag="PLF56_07295"
                     /EC_number="4.3.2.10"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_013161227.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_function: GO:0000107 - imidazoleglycerol-phosphate
                     synthase activity [Evidence IEA];
                     GO_process: GO:0000105 - histidine biosynthetic process
                     [Evidence IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="imidazole glycerol phosphate synthase subunit
                     HisF"
                     /protein_id="HOQ53402.1"
                     /translation="MSVAIRVIPCLDVHDGRVVKGVNFLNLRDAGDPVELAAAYGEQG
                     ADELTFLDISASSEGRETTHDMVRRTAETVFIPLCVGGGVRGVDDVDLLLRTGADKVG
                     INTGAIQRPQAIDEITERFGNQVLVLSLDARRESGRTSGFGVTTHGGRRPAGLDAIEW
                     VREAVDRGAGEILLNSMDADGTTDGFDLQMIEAVRRAVEVPVIASGGAGTVQHFVDAA
                     RAGADAVLAASVFHYGTLSITEVKQGLAEAGFPVRLTARS"
     gene            complement(903..1301)
                     /locus_tag="PLF56_07300"
     CDS             complement(903..1301)
                     /locus_tag="PLF56_07300"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_015070760.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="HIT domain-containing protein"
                     /protein_id="HOQ53403.1"
                     /translation="MDCLFCAIVAGDVPSRQVYADQAAVAFLDINPWKRGHTLVIPRR
                     HVNDLLAGDEALSEIVPAINATARLLVERLGADGINLLSNAGEVAGQEVFHLHVHLVP
                     RYAADPGLRALPIRVPGIDLDEVAATLGPS"
     gene            complement(1506..3038)
                     /locus_tag="PLF56_07305"
     CDS             complement(1506..3038)
                     /locus_tag="PLF56_07305"
                     /inference="COORDINATES: protein motif:HMM:TIGR02669.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_component: GO:0005575 - cellular_component [Evidence
                     IEA];
                     GO_function: GO:0003674 - molecular_function [Evidence
                     IEA];
                     GO_process: GO:0008150 - biological_process [Evidence
                     IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="SpoIID/LytB domain-containing protein"
                     /protein_id="HOQ53404.1"
                     /translation="MVRAGFAAVATALALVTAGLSAASPTADAAETAVRPLNGYLVVT
                     GAGWGHGKGMSQYGAYGAADAGLSHEKILSFYYPGTTLKKLAGGTTLRVWITADNDKT
                     LNVVPSAGQRVRDAAGHTYTLPTGSKYRQWRIKRSGSKRVLQYLNSKSAWVTRTTTLT
                     TTRVWSIDNPSRGLVTVRLPGGRNRDYRGKLALRFFGSGARTVNTVTMESYLRGVVPR
                     EMPTSWHAEAVQAQAVAARSYAARFQRSPQGSVYDLCDTAACQVYGGQDDETSGGNAA
                     VAATANRVVTYRGAVALTMFSSSNGGHSADGGLPYLVAKADPYDGRMHNQAWSIFLSA
                     ARIKSAYPSIGTLSSVRVSARDGHGWWGGRADSVVIAGSKSSLTVTGGAFKRTFGLKE
                     RLFGVFAGLKPGTANSDRWQNVEGGTTGSLGAPIANEVAVAGGLAARFSKGDLYWSKA
                     TGSRRLNGRLATAYRSAGGPASALGFPVADGAASGSSVVGVFQHGVIRCPASGDCLVQ
                     PG"
     gene            3726..>5094
                     /gene="ilvB"
                     /locus_tag="PLF56_07310"
     CDS             3726..>5094
                     /gene="ilvB"
                     /locus_tag="PLF56_07310"
                     /EC_number="2.2.1.6"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_020574770.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_component: GO:0005948 - acetolactate synthase complex
                     [Evidence IEA];
                     GO_function: GO:0003984 - acetolactate synthase activity
                     [Evidence IEA];
                     GO_process: GO:0009097 - isoleucine biosynthetic process
                     [Evidence IEA];
                     GO_process: GO:0009099 - valine biosynthetic process
                     [Evidence IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="biosynthetic-type acetolactate synthase large
                     subunit"
                     /protein_id="HOQ53405.1"
                     /translation="MSETMAPPMLTGAQALVESLERVGVEVIFGIPGGAILPAYDPLG
                     QSKLIRHILVRHEQGAGHAAEGYAVATGKVGVCIATSGPGATNLVTAIGDAYMDSVPI
                     VAITGQVNSSSIGTDAFQEADIRGITFPITKHSFLITRAEDIPSAIAAAFHIARTGRP
                     GPVLVDIAKDALATAAPFEWPEVIDLPGYHPVTQPHVKQIREAARLIGEAQRPVLYVG
                     GGVIKAQAADDLAKLVEITGIPVVTTLMARGSFPDSNELNMGMPGMHGTVAAVGALQR
                     ADLLIALGTRFDDRVTGRLDTFAPGAKVIHADIDPAEISKNRLADVPIVGDVKNVIDG
                     LNGVLRGADLPDITAWRRYLIALKNKYRVPTEPTHEGMLSPEFVIKRIGELTGPDAYY
                     AAGVGQHQMWSAQLLPFERPGRWLNSGGAGTMGYCVPAAMGAQVGQPDAVVWGIDGDG
                     CFQMTN"
BASE COUNT          808 a         1768 c         1739 g          779 t
ORIGIN      
        1 agggcgatgc cctcgctacc ctgagcgcat gagcgtcgcg atccgagtga ttccgtgcct
       61 ggacgtccac gacggccggg tggtgaaggg agtcaacttc ctcaacctgc gcgacgcggg
      121 cgacccggtc gaactggctg cggcctacgg tgaacagggt gccgatgaac tgacgttcct
      181 ggatatttcg gcctcgtccg agggccgcga gaccacccac gacatggtgc gccgcaccgc
      241 cgagacggtg ttcatcccgc tgtgcgtggg cgggggagtg cgcggggtcg acgacgtgga
      301 tctgctgctg cgcaccggtg ccgacaaggt gggcattaac accggggcga tccagcgtcc
      361 gcaggcgatc gacgagatca ccgagcgctt cggcaaccag gtgctggtgc tgtcgctgga
      421 cgcgcggcgg gagtcggggc ggacgtccgg attcggggtg accacgcacg gtggacgccg
      481 gccggccggc ctggacgcca tcgagtgggt tcgggaggcc gtcgaccgcg gcgccggcga
      541 gattctgctc aactcgatgg acgccgacgg caccaccgac gggttcgacc tacagatgat
      601 cgaggcggtc cggcgggcgg tcgaggtgcc ggtgatcgcc tcgggtggcg cgggcaccgt
      661 ccagcatttc gtggacgccg cccgtgccgg tgcggacgcc gtgctggccg cgtccgtgtt
      721 ccactacggc acgctcagca tcaccgaggt gaagcagggg ctggccgagg ccggcttccc
      781 ggtccggctg acagcgcggt cgtaggggtg ggccctgacc cctgtgatgg gggccttgcc
      841 ttgatccctg tgatgggggc cgccggcctg attcctgtga tgggggccgc cgaccacgaa
      901 cctcagctcg gccccagggt ggcggccacc tcgtccagat cgatgccggg cacgcggatg
      961 ggcagcgccc gaagcccggg gtcagcggcg taacgcggca ccaggtgcac gtgcaggtga
     1021 aagacctcct ggcccgcgac ctcgcccgcg ttgctgagca ggttgatgcc gtcggcgccc
     1081 agccgctcca ccagcagccg tgcggtggcg ttgatcgcgg ggacgatctc actgagcgcc
     1141 tcgtcgcctg ccaacagatc gttcacgtga cgtcgcggaa tcaccagggt gtgaccccgc
     1201 ttccatgggt tgatgtcgag aaaggccacc gcggcctgat cggcatagac ctggcgcgaa
     1261 ggaacatcac cggcgacgat agcgcagaac aggcaatcca tgacccgtaa tgtagaccgg
     1321 tcaccaaccc ctgcgcggat gatgcgcgac cagaacttac gggacgatgc gcgaccagaa
     1381 ctcacgggta ggcaggcaca gcggttgcca ccatcccagt gcgcgcgatg cgcgacggcc
     1441 ctgcgcgtag ctggccactt tgctgcgtcc cccctccctt caccgtaccg tcgagggccg
     1501 atccatcagc ccggctgcac caggcagtcc ccggaggccg ggcacctgat caccccgtgt
     1561 tggaacaccc ccacgacgga cgatccggac gctgcaccgt ccgcgaccgg aaagcccagc
     1621 gccgacgccg gcccgcccgc gctgcgatag gcagttgcga gccgcccgtt cagccgtcgg
     1681 gagccggtgg ccttcgacca gtagaggtcg cccttgctga accgcgccgc cagacctccg
     1741 gccaccgcaa cctcgttggc gatcggcgcg ccgagggatc cggtggtgcc gccttcgacg
     1801 ttctgccagc ggtcgctgtt ggccgtgccc ggtttgaggc cggcgaacac gccgaagagt
     1861 cgctccttca acccgaaggt gcgcttgaac gcgccgccgg tcaccgtgag gctgctcttc
     1921 gacccggcga tcaccaccga gtcggcgcgt ccgccccacc atccgtggcc gtcgcgcgcg
     1981 ctcacccgca cgctgctcag cgtgccgatc gacgggtagg ccgacttgat ccgtgccgcc
     2041 gagaggaaga tcgaccaggc ctggttgtgc attcgtccgt cgtaggggtc ggccttggcg
     2101 accagatagg gcaggccgcc gtccgcggag tgaccgccgt tgctcgagct gaacatggtc
     2161 agcgctaccg cgccccggta ggtcaccacc cggttcgccg tggccgcgac ggctgcgttg
     2221 ccgcccgagg tctcgtcgtc ctgcccgccg tacacctggc aggcggcggt gtcgcacagg
     2281 tcgtagaccg aaccctgcgg gctgcgctga aagcgggccg cgtacgaacg ggccgcgacg
     2341 gcctgcgcct gcaccgcctc ggcatgccac gaggtgggca tctcgcgtgg aaccacccca
     2401 cgcaggtagc tctccatggt cacggtgttc accgttctcg cgccggaacc gaagaagcgc
     2461 agcgccagct tgcctcggta gtcgcgattc ctgccgccgg gcaggcgcac ggtgaccaga
     2521 ccgcgggacg ggttgtcgat gctccacacc ctggtcgtgg tgagcgtggt ggtacgggtg
     2581 acccaggccg acttgctgtt caggtactgc agcacccgtt tcgaaccgga acgcttgatg
     2641 cgccactggc ggtacttcga accggtcggc agcgtgtagg tatggcctgc cgcgtcgcgc
     2701 acccgctgtc ccgcggacgg caccacgttc agcgtcttgt cgttgtcggc ggtgatccag
     2761 acccgcaggg tggtgccgcc ggccagcttt ttcagcgtcg tgcctgggta gtagaacgag
     2821 agaatctttt cgtgcgacag ccctgcgtcg gccgcgccgt aggcgccgta ctgcgacatg
     2881 cccttgccgt gaccccagcc ggcgccggtc accaccaggt agccgttcag cggacgcacg
     2941 gcagtctccg ccgcgtccgc ggtgggggag gcggcgctca ggccggcggt gaccagcgcc
     3001 agtgcggtcg caaccgcagc aaatccggct cgaaccatgg cgccacctcg ggggagaaga
     3061 gcaattccgc ccatggttgc ccggttctcg accggacaca accgtgttct caaccccggg
     3121 ttgcgagtgc gggcgactcg tggcgcatgg ggggcgcacc tcgccggggg caggtcaccc
     3181 gctgcccgcg accctcgccc cgagccgcgg tcaaggccat tggggacatg ctgaccaatc
     3241 gctggtcgat cgtcgagacc ctcgtagtgg gggttcgaca cgctcggcca acgcggctcg
     3301 ccgcattgat cagcgtcctg tcgcagtgcg agtcgcggct gacagcggct gggtttcagc
     3361 accgactgcg gtaccaaaac ccacccgctc ccgaagagtg aaccgagacc agctctccga
     3421 gcggtgctcg tggaccgcgc ggggcatggc gggcgcccac ttgggcgtgg ccgacgacgc
     3481 ctggctgccg ggcgggcgtc gtcatcggat gccgcgaacc tcgtccacat cctgaaaaca
     3541 ccggtccgaa agttgacacc gacccgtcca cggcgaaaac tgttgccgtg cggaacctca
     3601 acctcgtact tgtgtagcgt gtcagccgac tagtcgaccg acacgcaccc tcgcctgccg
     3661 acgcagcggg ggttttttca tgcacagcct gagccgcaac caccacctgc tgaaagggga
     3721 cggagatgtc cgagaccatg gcaccaccga tgctgaccgg ggcgcaggcc ctcgtcgagt
     3781 ccctggagcg ggtcggcgta gaggtcatct tcggcatccc gggcggcgcg atcctgccgg
     3841 cctatgaccc gctcggccag tccaaactga tccggcacat cctggtgcgg cacgagcagg
     3901 gcgccgggca tgcggccgag gggtacgcgg tcgcgaccgg caaggtcggc gtgtgcatcg
     3961 ccacctccgg cccgggcgcc accaacctgg tcaccgcgat cggcgacgcc tacatggatt
     4021 cggtgccgat cgtcgcgatc accggccagg tcaacagcag ttcgatcggc acggacgcct
     4081 ttcaagaggc cgacatccgg ggcatcacct ttccgatcac caagcacagc tttctgatca
     4141 cacgggcaga agacattccc agcgccatcg ccgccgcctt tcacatcgct cgcaccggac
     4201 gtccgggccc ggtgctggtc gacatcgcca aggacgccct ggcgacggcc gcgccgttcg
     4261 agtggcccga ggtgatcgac ctgcccggct accacccggt cacccagccc cacgtcaagc
     4321 agattcgcga agcggcccgg ctgatcggtg aggcgcagcg tccggtgctg tacgtcggcg
     4381 gcggcgtgat caaggcccag gccgccgacg atctggccaa gctggtcgag atcaccggca
     4441 tccccgtggt caccaccctg atggcgcggg ggtcctttcc cgacagcaac gaactcaaca
     4501 tgggcatgcc gggcatgcac ggcacggtcg ccgcggtcgg cgccctgcag cgcgccgacc
     4561 tgttgatcgc cctcggcacc cgcttcgacg accgggtgac cggcaggctg gacaccttcg
     4621 cacccggcgc gaaggtgatt cacgccgaca tcgacccggc cgagatcagc aagaaccggc
     4681 tggccgacgt gccgatcgtg ggcgatgtga agaacgtcat cgacggcctg aacggggtgc
     4741 tgcgcggcgc cgacctgccc gacatcaccg cctggcggcg ctacctgatc gcgctcaaga
     4801 acaagtaccg ggtgccgacc gaacccaccc acgagggcat gctgtcgccc gagttcgtga
     4861 tcaagcggat cggcgagctg accggtcccg acgcgtacta cgccgccggt gtgggccagc
     4921 accagatgtg gtcggcgcaa ctgctgccct tcgaacgtcc gggccgctgg ctcaactcgg
     4981 gcggcgcggg caccatgggc tactgcgtgc ccgcggcaat gggtgcgcag gtgggtcaac
     5041 cggacgcggt ggtgtggggc atcgacggcg acggctgttt tcagatgacg aacc
//
LOCUS       DAOSUG010000188         5079 bp    DNA     linear   ENV 10-JUN-2024
DEFINITION  MAG TPA_asm: Micropruina sp. isolate AMR_MDS_2195
            NODE_7274_length_5079_cov_4.171576, whole genome shotgun sequence.
ACCESSION   DAOSUG010000188 DAOSUG010000000
VERSION     DAOSUG010000188.1
DBLINK      BioProject: PRJNA925477
            BioSample: SAMN32864262
            Sequence Read Archive: SRR4236673
KEYWORDS    WGS; ENV; Metagenome Assembled Genome; MAG; Third Party Data; TPA;
            TPA:assembly.
SOURCE      Micropruina sp. (wastewater metagenome)
  ORGANISM  Micropruina sp.
            Bacteria; Bacillati; Actinomycetota; Actinomycetes;
            Propionibacteriales; Nocardioidaceae; Micropruina.
REFERENCE   1  (bases 1 to 5079)
  AUTHORS   Abdulkadir,N., Saraiva,J.P., Zhang,J., Stolte,S., Gillor,O.,
            Harms,H. and Rocha,U.
  TITLE     Genome-centric analyses of 165 metagenomes show that mobile genetic
            elements are crucial for the transmission of antimicrobial
            resistance genes to pathogens in activated sludge and wastewater
  JOURNAL   Microbiol Spectr 12 (3), e0291823 (2024)
   PUBMED   38289113
REFERENCE   2  (bases 1 to 5079)
  AUTHORS   Abdulkadir,N.
  TITLE     Direct Submission
  JOURNAL   Submitted (24-MAR-2023) Environmental Microbiology, Helmholtz
            Center for Environmental Research -UFZ, Permoserstr 15, Leipzig
            04318, Germany
COMMENT     The annotation was added by the NCBI Prokaryotic Genome Annotation
            Pipeline (PGAP). Information about PGAP can be found here:
            https://www.ncbi.nlm.nih.gov/genome/annotation_prok/
            
            ##Genome-Assembly-Data-START##
            Assembly Method        :: SPAdes v. 3.11.1
            Genome Representation  :: Full
            Expected Final Version :: Yes
            Genome Coverage        :: Not Applicable
            Sequencing Technology  :: Illumina MiSeq
            ##Genome-Assembly-Data-END##
            
            ##Genome-Annotation-Data-START##
            Annotation Provider               :: NCBI
            Annotation Date                   :: 05/04/2023 04:57:10
            Annotation Pipeline               :: NCBI Prokaryotic Genome
                                                 Annotation Pipeline (PGAP)
            Annotation Method                 :: Best-placed reference protein
                                                 set; GeneMarkS-2+
            Annotation Software revision      :: 6.5
            Features Annotated                :: Gene; CDS; rRNA; tRNA; ncRNA
            Genes (total)                     :: 2,776
            CDSs (total)                      :: 2,750
            Genes (coding)                    :: 2,714
            CDSs (with protein)               :: 2,714
            Genes (RNA)                       :: 26
            tRNAs                             :: 24
            ncRNAs                            :: 2
            Pseudo Genes (total)              :: 36
            CDSs (without protein)            :: 36
            Pseudo Genes (ambiguous residues) :: 0 of 36
            Pseudo Genes (frameshifted)       :: 2 of 36
            Pseudo Genes (incomplete)         :: 32 of 36
            Pseudo Genes (internal stop)      :: 2 of 36
            ##Genome-Annotation-Data-END##
FEATURES             Location/Qualifiers
     source          1..5079
                     /organism="Micropruina sp."
                     /mol_type="genomic DNA"
                     /submitter_seqid="NODE_7274_length_5079_cov_4.171576"
                     /isolate="AMR_MDS_2195"
                     /isolation_source="Activated sludge of municipal
                     wastewater treatment plants"
                     /db_xref="taxon:2737536"
                     /environmental_sample
                     /geo_loc_name="Canada"
                     /lat_lon="49.25 N 123.1 W"
                     /collection_date="2015-06-16"
                     /metagenome_source="wastewater metagenome"
                     /note="metagenomic"
     gene            complement(35..577)
                     /locus_tag="PLF56_07315"
     CDS             complement(35..577)
                     /locus_tag="PLF56_07315"
                     /inference="COORDINATES: ab initio
                     prediction:GeneMarkS-2+"
                     /note="Derived by automated computational analysis using
                     gene prediction method: GeneMarkS-2+."
                     /codon_start=1
                     /transl_table=11
                     /product="hypothetical protein"
                     /protein_id="HOQ53406.1"
                     /translation="MLRKSVVLIVGLFAMIAMSAVAAPAQAASHTWCDPHSSTSGGRC
                     ITVSKPKYHEIYVDTIPIRNKTKKIKVHAYCKWSDTATWGMKAGAEVSASIEGKIFGL
                     AKATAGVTLHTEVSMSASLAREVAGDFVLKPGQTVFCHRTVGYYSFTTVISEWNTTKS
                     WKKTMTSKVPYTMGATFGDR"
     gene            complement(673..1692)
                     /locus_tag="PLF56_07320"
     CDS             complement(673..1692)
                     /locus_tag="PLF56_07320"
                     /inference="COORDINATES: protein motif:HMM:NF013775.2"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="phosphotransferase"
                     /protein_id="HOQ53407.1"
                     /translation="MTKPFGELTARTQVDRLRRLVRSALPMWNLPDARITLLYHGYNT
                     TFRVDAADGRRFALRVNTQPHKTIAHLRGEVTWLAHLSAQTELHVPTPQRTREEALFT
                     VVPSRDHVGGLPMVLFSWLDGRNLGIHWSRTEARAVGVAMAILHQNAAHWTMPAGAAF
                     SPFDDVLTDLPNRLGDHPALDARARSVLTTAYDRAQALQNEAFAADTVIALHADLHGE
                     NLKWHRGRLRVFDFDDAGLGVPALDLAICAYYLRDDAGAEEELKAGYSTVRSLPPVTD
                     EQHEAMVAGRNLLIVSDLIDQTNARLRALRPSFVAASVERLQRWLDTGRWVRRVDEQP
                     TVGQV"
     gene            complement(1927..2961)
                     /gene="meaB"
                     /locus_tag="PLF56_07325"
     CDS             complement(1927..2961)
                     /gene="meaB"
                     /locus_tag="PLF56_07325"
                     /EC_number="3.6.5.-"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_002541495.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_function: GO:0003924 - GTPase activity [Evidence IEA];
                     GO_function: GO:0005525 - GTP binding [Evidence IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="methylmalonyl Co-A mutase-associated GTPase
                     MeaB"
                     /protein_id="HOQ53408.1"
                     /translation="MRRMTPVELAEQVRQGSRPHIARAITLVESSRADHREQAKQLLR
                     LLRPASGNSVRVGITGVPGAGKSTFIDALGVRLIDQGHRIAVLAVDPSSSRTGGSILG
                     DRTRMAALTESENAYVRPSPSGGHLGGVARATRESMIVMEAAGFDVILVETVGVGQSE
                     VAVSGMVDTFLLITLARAGDQLQGIKRGILEMADVITVNKADGDHEGEARVTARELTI
                     AMKLISSDPNARRPPVLTSSSYTGKGLDEVWQAVLDHRAYLQSHGGLEPRRAKQQIEW
                     LWSLVEGEVLDGVRNSPRVRTLRPGIEAEIAANEISALEGAEDLVRAFVSDVPVVWPA
                     PAVEGVDTTG"
     assembly_gap    3212..3311
                     /estimated_length=100
                     /gap_type="within scaffold"
                     /linkage_evidence="paired-ends"
     gene            complement(<3312..3570)
                     /locus_tag="PLF56_07330"
     CDS             complement(<3312..3570)
                     /locus_tag="PLF56_07330"
                     /inference="COORDINATES: ab initio
                     prediction:GeneMarkS-2+"
                     /note="Derived by automated computational analysis using
                     gene prediction method: GeneMarkS-2+."
                     /codon_start=1
                     /transl_table=11
                     /product="hypothetical protein"
                     /protein_id="HOQ53409.1"
                     /translation="MSSVQPSSGGRRVSQPAVVISANVRISQPAVVIPAPPVIPAPPV
                     IPANAGISVGRVGTFGCWRGEGRGRVEVTSAHSSSRHRPSSR"
     gene            complement(3584..>5079)
                     /locus_tag="PLF56_07335"
     CDS             complement(3584..>5079)
                     /locus_tag="PLF56_07335"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_015069853.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_function: GO:0016866 - intramolecular transferase
                     activity [Evidence IEA];
                     GO_function: GO:0031419 - cobalamin binding [Evidence
                     IEA]"
                     /codon_start=3
                     /transl_table=11
                     /product="methylmalonyl-CoA mutase family protein"
                     /protein_id="HOQ53410.1"
                     /translation="MPKFNSISISGYHMQEAGATADIEMAYTLADGVEYIRAGESVGL
                     KVDQFAPRLSFFWAVGMNFFMEVAKFRAARLLWARLVRDFGPKNPKSMSLRTHSQTSG
                     WSLTAQDVFNNVGRTCLEAMAATQGHTQSLHTNALDEAIALPTDFSARIARNTQLFIQ
                     QESGTTRVVDPWAGSAYVERLTYELAAKAWELISEVEAAGGMAKAIEAGIPKMRIEEA
                     AARTQARIDSGRQPLIGVNKYIVEHDEPPEVLKVDNAKVRDEQIAKLQRLRGQRDEAV
                     TKAALEKLSWAAANPDPTDPERNLLHLAIDAARKSASVGEISDALEQVFGRYTAQIRT
                     ISGVYRSETGQTSGVEKARALVEDFEKAEGRRPRILVAKMGQDGHDRGQKVISTAFAD
                     LGFDVDVGPLFQTPAEVARQAVEADVHVVGVSSLAAGHLTLVPALRAELDKLGRDDMM
                     IVVGGVIPAQDFDELRAKGADAIYPPGTIIPEAAVELLQKLRDRLED"
BASE COUNT          767 a         1718 c         1677 g          817 t
ORIGIN      
        1 cgtccgagta tcagcgccga tcagcgaaca gcattcaccg gtcgccgaat gtggcgccca
       61 tcgtgtaggg aaccttgctc gtcatcgtct ttttccacga cttcgtcgtg ttccattcac
      121 tgatcaccgt ggtgaagctg tagtagccga ccgtgcgatg gcagaacacc gtctgacctg
      181 gcttgagcac gaaatcgccc gccacctcgc gagccagcga ggccgacatg ctgacctccg
      241 tgtgtagcgt cacgccggcg gtggccttcg cgagcccgaa gatcttgccc tcgatcgagg
      301 ccgagacctc ggcgccggcc ttcatgcccc aggtggccgt gtccgaccac ttgcagtagg
      361 catgcacctt gatcttcttg gtcttgttgc ggatcggaat ggtgtcgaca tagatctcgt
      421 ggtacttcgg cttgctgacc gtgatgcacc ggccaccgct ggtgctgctg tgcggatcgc
      481 accaggtgtg gctagccgcc tgcgcgggtg cggcaaccgc cgacattgcg atcatggcga
      541 acagccccac gatcagtacc actgactttc gaagcacgac cgattctcct gacgggggat
      601 gacggctgcc aaggtagtcc caggcacagc ggtgcgtcaa cggtacgttc cacaactgtc
      661 gtgctcgtac cgctatacct gccccaccgt tggttgctca tccacccggc gcacccaacg
      721 accggtgtcg agccagcgct gcagacgctc gacgctggcg gccacgaaac tgggcctgag
      781 tgcgcgcagg cgggcgttgg tctggtcgat caggtcgctg acgatcagca ggttgcgtcc
      841 ggcgaccata gcttcgtgct gctcgtcggt gaccggcggc agcgagcgga cggtgctgta
      901 ccccgccttc agttcctcct ccgccccggc gtcgtcgcgc aggtagtagg cgcagatggc
      961 taggtcgagc gccggcacgc cgagtccggc gtcatcgaag tcgaagaccc tgagtcgtcc
     1021 ccggtgccac ttgaggttct cgccgtgcag gtcggcgtgc agggcgatga cggtatcggc
     1081 ggcgaaggcc tcattctgca gggcctgggc gcggtcgtag gccgtcgtca acacggaccg
     1141 cgcacgagcg tccagtgccg ggtgatcccc gagccggttg ggcaggtccg tgagcacgtc
     1201 gtcgaacgga ctgaacgccg cacccgcagg catcgtccag tgagcagcgt tctggtgcag
     1261 gatggccatg gcgactccga cagcgcgggc ctcggtgcgg ctccagtgaa tgccgaggtt
     1321 gcgcccgtcc agccacgaga acagcaccat gggcaggcca ccgacatggt cgcggcttgg
     1381 caccacggtg aacaacgcct cctcccgggt gcgctgcggg gtcggcacgt gcagttcggt
     1441 ctgcgcgctc aggtgggcca gccaggtgac ctcaccccgt aggtgggcga tggtcttgtg
     1501 cggctgggtg ttgacccgta gggcaaagcg ccggccgtcg gcggcatcga cccgaaaggt
     1561 cgtgttgtac ccgtggtaga gcagggtgat ccgggcgtcg ggcaggttcc acatcggtag
     1621 tgcggagcgg accagcctgc gcaacctgtc gacctgcgtc cgtgctgtca gttcaccgaa
     1681 cggcttggtc atgcccggca gcttaagcca ggagccaccg aggtgcgccg tcgcgagcgg
     1741 caccaggcgg gtgggccggc aggacgtacc gcgcaagggc atacccggcg tcggatgagc
     1801 gtggccgacg cagccaggac gcccgagtgg gggtcgcgca gcaggcgcag atcggtggat
     1861 ccgggtggag cgcggagccg ggcgagttct ggggcgtctt ttcaatcggg gcgcggtgcc
     1921 acgccgtcag ccggtggtgt cgaccccctc gacggcgggc gcgggccaga ccaccggcac
     1981 gtccgagacg aaggcccgca ccaggtcctc cgctccttcg agcgccgaga tctcgttggc
     2041 cgcgatctcg gcctcgatgc ccggccgcag ggtgcgcacg cggggagagt tgcggacgcc
     2101 gtcgagcacc tcgccctcga ccaacgacca caaccattcg atctgctgct tcgcccggcg
     2161 gggttccagg ccgccgtgcg actgcaggta ggcgcggtgg tccaggaccg cctgccagac
     2221 ctcgtccaga cccttgccgg tgtatgacga cgaggtgagc accggtggac gccgcgcgtt
     2281 cgggtcggag ctgatcagct tcatcgcgat ggtcagctcg cgagcggtca cccgggcctc
     2341 gccctcgtgg tcgccgtcgg ccttgttgac cgtgatcacg tcggccatct cgaggatgcc
     2401 gcgcttgatg ccctgcagtt gatcgccggc ccgagccagg gtgatcagca gaaacgtgtc
     2461 gaccatgccc gaaaccgcca cctcggactg cccaaccccg accgtctcga ccagaatcac
     2521 gtcgaagccc gctgcctcca tcacgatcat cgactcgcgg gtcgcccggg caacgccacc
     2581 cagatgcccc ccggacggcg acggacgcac gtacgcgttc tcggactcgg tcaaagccgc
     2641 catgcgggtg cggtcgccga ggatcgagcc accggtgcgc gacgaagacg ggtcgacggc
     2701 caacacggcg atgcggtgcc cctggtcgat cagccggacc cccagcgcgt cgatgaaggt
     2761 ggatttaccc gcgcccggca ctccggtgat gccgacccgc accgagttgc cgctggccgg
     2821 ccgcaacaac cgcagcaact gcttggcctg ctcacggtga tcggcacggg acgactcgac
     2881 caaagtgatg gcccgcgcga tgtgtggacg ggaaccctgc cgcacctgct cggccagctc
     2941 caccggggtc atccttcgca tgtgctcacc ctctcaaact cttgcggctc gtggtctcgt
     3001 ccgacccaac gggcggtcgg tacagacgag agttgactcc gagatcccgg cgtccaccgg
     3061 gatgacgaat gcgccgacgt gacctccacc cgacccccgc ccccgccacg acagcgccca
     3121 gaggtgccga cccgaccgac cgagatcccg gcattcgccg ggatgacggg cggtgccggg
     3181 atgacgaatg cgccgacgtg acctcgaccc gnnnnnnnnn nnnnnnnnnn nnnnnnnnnn
     3241 nnnnnnnnnn nnnnnnnnnn nnnnnnnnnn nnnnnnnnnn nnnnnnnnnn nnnnnnnnnn
     3301 nnnnnnnnnn ngccgggatg acgggcggtg ccgggatgac gaatgcgccg acgtgacctc
     3361 gacccgaccg cggccctcgc cgcgccagca cccaaaggtg ccgacccgac cgaccgagat
     3421 cccggcattc gccgggatga cgggcggtgc cgggatgacg ggcggtgccg ggatgacgac
     3481 ggcgggttgc gagatcctga cgttcgccga gatgacgacg gcgggttgcg agacccggcg
     3541 tccgccggat gacggctgca ccgacgacac ctcgaacgtc tgctcagtcc tccaaccgat
     3601 cccgcagctt ctgcagcaac tccaccgcag cctccgggat gatcgtgccg ggcgggtaga
     3661 tcgcgtccgc ccccttggcc cgcagctcgt cgaagtcctg cgccgggatc acgccgccga
     3721 ccacgatcat catgtcgtcg cggccgagct tgtcgagttc ggcccgcagc gccggcacca
     3781 gggtcaggtg acctgcggcc agcgacgaca cgccgaccac gtgcacgtcg gcctccaccg
     3841 cctgccgcgc gacctcggcc ggcgtctgga acagcgggcc gacatcgacg tcgaagccga
     3901 ggtcggcgaa ggccgtcgag atcaccttct ggccgcggtc gtgcccgtcc tggcccatct
     3961 tggccaccag gatgcgcgga cgccgtccct cggccttctc gaagtcctcc accagcgccc
     4021 gcgccttctc gacgccgctg gtctgaccgg tctcgctgcg gtacacgcca ctgatcgtac
     4081 ggatctgtgc ggtgtagcgc ccgaagacct gctcgagggc gtccgagatc tcaccgacgg
     4141 aggccgactt gcgcgcggcg tcgatggcca ggtgcagcag gttgcgttcg ggatcggtcg
     4201 ggtcggggtt ggccgccgcc cagctcagct tctcgagcgc cgccttggtg accgcctcgt
     4261 cgcgctgccc acgcagccgc tgcagcttgg cgatctgctc gtcgcgcacc ttggcgttgt
     4321 cgaccttcag cacctccggt ggctcgtcgt gctcgacgat gtacttgttg acgccgatca
     4381 gcggctgccg tccggagtcg atccgggcct gggtgcgggc cgcggcctct tcgatgcgca
     4441 tcttgggaat gccggcctcg atcgccttgg ccatgccgcc ggccgcctcg acctcgctga
     4501 tcagttccca cgccttcgcg gccagctcgt aggtgagccg ctcgacgtag gccgaacccg
     4561 cccaggggtc gaccacccgc gtggtgcccg actcctgctg aatgaacagc tgggtgttgc
     4621 gggcgatccg ggccgagaag tcggtcggca gcgcgatcgc ctcgtccagg gcgttggtgt
     4681 gcagcgactg ggtgtggccc tgcgtggcgg ccatcgcctc caggcaggtg cgtccgacgt
     4741 tgttgaacac gtcctgcgcg gtgagcgacc agcccgaggt ctgcgaatgg gtgcgcagtg
     4801 acatggactt ggggttcttc ggcccgaaat cgcggacgag gcgcgcccac agcaaccgcg
     4861 cggcccggaa cttcgccacc tccatgaaga agttcatgcc gacggcccag aagaagctca
     4921 gccgcggcgc gaactggtcg accttcaggc ccaccgattc gcctgcgcgg atgtactcca
     4981 cgccgtcggc cagcgtgtag gccatctcga tgtcggccgt cgcgccggcc tcttgcatgt
     5041 ggtagcccga gatcgagatc gagttgaact tgggcatgt
//
LOCUS       DAOSUG010000189         5067 bp    DNA     linear   ENV 10-JUN-2024
DEFINITION  MAG TPA_asm: Micropruina sp. isolate AMR_MDS_2195
            NODE_7301_length_5067_cov_4.373504, whole genome shotgun sequence.
ACCESSION   DAOSUG010000189 DAOSUG010000000
VERSION     DAOSUG010000189.1
DBLINK      BioProject: PRJNA925477
            BioSample: SAMN32864262
            Sequence Read Archive: SRR4236673
KEYWORDS    WGS; ENV; Metagenome Assembled Genome; MAG; Third Party Data; TPA;
            TPA:assembly.
SOURCE      Micropruina sp. (wastewater metagenome)
  ORGANISM  Micropruina sp.
            Bacteria; Bacillati; Actinomycetota; Actinomycetes;
            Propionibacteriales; Nocardioidaceae; Micropruina.
REFERENCE   1  (bases 1 to 5067)
  AUTHORS   Abdulkadir,N., Saraiva,J.P., Zhang,J., Stolte,S., Gillor,O.,
            Harms,H. and Rocha,U.
  TITLE     Genome-centric analyses of 165 metagenomes show that mobile genetic
            elements are crucial for the transmission of antimicrobial
            resistance genes to pathogens in activated sludge and wastewater
  JOURNAL   Microbiol Spectr 12 (3), e0291823 (2024)
   PUBMED   38289113
REFERENCE   2  (bases 1 to 5067)
  AUTHORS   Abdulkadir,N.
  TITLE     Direct Submission
  JOURNAL   Submitted (24-MAR-2023) Environmental Microbiology, Helmholtz
            Center for Environmental Research -UFZ, Permoserstr 15, Leipzig
            04318, Germany
COMMENT     The annotation was added by the NCBI Prokaryotic Genome Annotation
            Pipeline (PGAP). Information about PGAP can be found here:
            https://www.ncbi.nlm.nih.gov/genome/annotation_prok/
            
            ##Genome-Assembly-Data-START##
            Assembly Method        :: SPAdes v. 3.11.1
            Genome Representation  :: Full
            Expected Final Version :: Yes
            Genome Coverage        :: Not Applicable
            Sequencing Technology  :: Illumina MiSeq
            ##Genome-Assembly-Data-END##
            
            ##Genome-Annotation-Data-START##
            Annotation Provider               :: NCBI
            Annotation Date                   :: 05/04/2023 04:57:10
            Annotation Pipeline               :: NCBI Prokaryotic Genome
                                                 Annotation Pipeline (PGAP)
            Annotation Method                 :: Best-placed reference protein
                                                 set; GeneMarkS-2+
            Annotation Software revision      :: 6.5
            Features Annotated                :: Gene; CDS; rRNA; tRNA; ncRNA
            Genes (total)                     :: 2,776
            CDSs (total)                      :: 2,750
            Genes (coding)                    :: 2,714
            CDSs (with protein)               :: 2,714
            Genes (RNA)                       :: 26
            tRNAs                             :: 24
            ncRNAs                            :: 2
            Pseudo Genes (total)              :: 36
            CDSs (without protein)            :: 36
            Pseudo Genes (ambiguous residues) :: 0 of 36
            Pseudo Genes (frameshifted)       :: 2 of 36
            Pseudo Genes (incomplete)         :: 32 of 36
            Pseudo Genes (internal stop)      :: 2 of 36
            ##Genome-Annotation-Data-END##
FEATURES             Location/Qualifiers
     source          1..5067
                     /organism="Micropruina sp."
                     /mol_type="genomic DNA"
                     /submitter_seqid="NODE_7301_length_5067_cov_4.373504"
                     /isolate="AMR_MDS_2195"
                     /isolation_source="Activated sludge of municipal
                     wastewater treatment plants"
                     /db_xref="taxon:2737536"
                     /environmental_sample
                     /geo_loc_name="Canada"
                     /lat_lon="49.25 N 123.1 W"
                     /collection_date="2015-06-16"
                     /metagenome_source="wastewater metagenome"
                     /note="metagenomic"
     gene            complement(<1..350)
                     /locus_tag="PLF56_07340"
     CDS             complement(<1..350)
                     /locus_tag="PLF56_07340"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_012920503.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_function: GO:0005524 - ATP binding [Evidence IEA];
                     GO_process: GO:0009058 - biosynthetic process [Evidence
                     IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="Mur ligase domain-containing protein"
                     /protein_id="HOQ53411.1"
                     /translation="MADQPVRRPPTLAAAPVRLSGLLPGAGELSVTGVSLDSRRVGPG
                     ELYAALPGQHTHGARFAADAAARGAVAVLTDAEGQALVGDLLPVIVVDDPRLALAEVA
                     AVVHGRPAEAMRTFG"
     gene            complement(362..2194)
                     /locus_tag="PLF56_07345"
     CDS             complement(362..2194)
                     /locus_tag="PLF56_07345"
                     /inference="COORDINATES: protein motif:HMM:NF013100.2"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_component: GO:0016020 - membrane [Evidence IEA];
                     GO_function: GO:0008658 - penicillin binding [Evidence
                     IEA];
                     GO_process: GO:0009252 - peptidoglycan biosynthetic
                     process [Evidence IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="penicillin-binding protein 2"
                     /protein_id="HOQ53412.1"
                     /translation="MTLKSSTQRRLKSMGRVRWPLAQPVKRMRVLLLMIAMVFSLCAG
                     RALQVQAFDAKAYATEAADRMQESLVLPAARGDITDRYGAVLAYTEQTVNVIADPKAI
                     RTNGKMDLQPMTARDKEIAAAAPQRIAQLLAKYLGNTPESYLPELTDQKSSWKVIAKR
                     VPANTFEQLQAELKAAGLLGLSRVTAPTRRYPNGTLAANLIGWVNEQGQAAGGLELSL
                     EKLLRGTDGKEVYDSSPNGKIPLGDNVLIPAQNGHNFGLTIDSSLQLMAEQRLAKTLA
                     DTGAQAAAVIVMDPRTGEVLVMANGPSFDSNEPGKGDAANMGNRAAMSVYEPGSVQKI
                     LTFAALIDSGTINPDDKVKFPGFIKQDQFKITDWWNHGTITLRARGIFAKSSNVGTIT
                     LARKMNKEKLRAYYASFGLGQSTNSGIGGEATGYLPAATMPNYTRDGIAFGTSLSVTA
                     LQNATAISSVVNGGVYHAPTVISSMDGQPFTASKQDPTPRRVISESTSEQVRSLMEQQ
                     ALNQTSKSFSINGYRIGTKTGTARLVSADCKCYRGESVVSTVGVAPIDDPQVLVYAVV
                     WNPKRNGASGASVAGPLMHDVLALALPRYAVEPSGAKGVKLPLQ"
     gene            complement(2191..2865)
                     /locus_tag="PLF56_07350"
     CDS             complement(2191..2865)
                     /locus_tag="PLF56_07350"
                     /inference="COORDINATES: ab initio
                     prediction:GeneMarkS-2+"
                     /note="Derived by automated computational analysis using
                     gene prediction method: GeneMarkS-2+."
                     /codon_start=1
                     /transl_table=11
                     /product="hypothetical protein"
                     /protein_id="HOQ53413.1"
                     /translation="MTALEAPRRSPEEKPRRWGLTQVPIPERRMANLPFVLTLAVFLT
                     LGMVGLLLLNTALQEQGFAVQNQQKAADQLGYRVAELESEITEARSSTRLAEEATKLG
                     MVPNPYPVYLSLPAGTVIGDPTRISGYELPDVRYRTPEQLAALTKQAADTEAKRLAEV
                     AARQKAAAEAKKKAAEEKAKAEAAAKKKADETKKAEAAAKKKADEQQKAEAAKKKTGK
                     KNGTDR"
     gene            complement(2862..3827)
                     /gene="rsmH"
                     /locus_tag="PLF56_07355"
     CDS             complement(2862..3827)
                     /gene="rsmH"
                     /locus_tag="PLF56_07355"
                     /EC_number="2.1.1.199"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_012920499.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_function: GO:0016434 - rRNA (cytosine)
                     methyltransferase activity [Evidence IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="16S rRNA (cytosine(1402)-N(4))-methyltransferase
                     RsmH"
                     /protein_id="HOQ53414.1"
                     /translation="MDGQQAVAHVPVMCARIVELLSVPLQAADSRYVDCTLGLGGHAE
                     AILEACPRATLIGIDRDQHALRLAGERLARFGDRVQLYQARYDELPEVLAEAGRPRVQ
                     AILADLGLSSMQIDRRERGFAYSVDAPLDMRMDDTQTLTAADLVNQASAAELSMILRR
                     FGEERFADRIARRVVAERADEPFTTSGRLVRVIEGAIPAAARATGGHPAKRTFQALRI
                     AVNDELVALAGLLPAAIAALAPGGRLAVLAYHSGEDRLVKNALRDAATDRAPRGLPAV
                     PDGLRAQLMLLTRGAERPGPDEVQVNPRAASARLRVGERIVEVAA"
     gene            complement(3977..4405)
                     /gene="mraZ"
                     /locus_tag="PLF56_07360"
     CDS             complement(3977..4405)
                     /gene="mraZ"
                     /locus_tag="PLF56_07360"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_005961682.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_function: GO:0043565 - sequence-specific DNA binding
                     [Evidence IEA];
                     GO_process: GO:0051302 - regulation of cell division
                     [Evidence IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="division/cell wall cluster transcriptional
                     repressor MraZ"
                     /protein_id="HOQ53415.1"
                     /translation="MFLGTHTPKLDDKGRFFLPAKFREELNGGLVITRQPDRCLAIFT
                     PAAYQAAADKMIAGSSSLAKVRSLQRMFASGADSSTPDGQGRITVPEHLRTYAGLQRE
                     IVVIGALDHIEVWDAEAWASYAAAQEEAFAQMDEEVFGPG"
     gene            4532..>5067
                     /locus_tag="PLF56_07365"
     CDS             4532..>5067
                     /locus_tag="PLF56_07365"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_014846931.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="AAA family ATPase"
                     /protein_id="HOQ53416.1"
                     /translation="MERTAASRVDDVQAVAQSIRGAVSTVIEGKPEAIELALVALFSS
                     GHLLIEDVPGVGKTMLAKALARSISGTAQRIQFTPDLLPSDITGVSIFNSQRHDFEFK
                     PGGVFANVVIGDEINRASPKTQSALLEAMEEHQVTVDGTTHPLPDPFLVVATQNPIEM
                     EGTYPLPEAQRDRFMLRIS"
BASE COUNT          699 a         1790 c         1721 g          857 t
ORIGIN      
        1 ccgaacgtgc gcatcgcctc ggccggacgt ccgtgcacca cggccgccac ctcggccagg
       61 gccaggcgcg ggtcgtcgac cacgatcacc ggcagcagat cgcccaccag ggcctgcccc
      121 tcggcgtcgg tgagcaccgc caccgcaccc cgggcggccg cgtccgcagc gaaccgggcg
      181 ccgtgcgtgt gctggccggg cagggccgcg tacagctcgc cggggcccac ccggcgcgaa
      241 tccaggctca cgccggtcac ggacagctcg cccgcaccgg gcagcaaccc gctcaggcga
      301 accggtgcgg cggcgagggt cggtggccgg cgcaccggct ggtcagccat gcctcaccct
      361 attactggag cgggagcttg acgcccttgg caccggacgg ctccaccgcg taccggggca
      421 gcgccagggc gagcacgtcg tgcatcagcg ggccggcgac gctggcgccc gaggcgccgt
      481 tgcgcttggg gttccagacc accgcataga ccagcacctg cgggtcatcg atcggcgcca
      541 ccccgaccgt cgacaccacc gactcgccgc ggtagcactt gcagtcggcg ctgaccaggc
      601 gggccgtacc ggtcttggtg ccgatccggt agccgttgat cgagaacgac ttgctggtct
      661 ggttcaacgc ctgttgctcc atcaacgaac gcacctgctc cgaggtggac tcgctgatca
      721 cccggcgcgg cgtcgggtcc tgcttggacg cggtgaacgg ctgcccgtcc atgctcgaga
      781 tcacggtcgg ggcgtggtac acgccgccgt tgaccacgga cgagatcgcg gtcgcgttct
      841 gcagggcggt caccgagagg ctggtgccga acgcgatgcc gtcgcgggtg tagttcggca
      901 tggtcgccgc cggcaggtag ccggtcgcct cgccgccgat gccactgttg gtgctctggc
      961 ccagcccgaa cgaggcgtag taggcgcgca gcttctcttt gttcatcttg cgggccagcg
     1021 tgatcgtgcc gacgttggac gacttggcga agatgccccg agcccgcagg gtgatcgtgc
     1081 cgtggttcca ccagtcggtg atcttgaact ggtcctgctt gatgaagccg ggaaacttca
     1141 ccttgtcgtc gggattgatg gtgcccgagt cgatcagcgc ggcgaaggtc aggatcttct
     1201 gcaccgagcc cggctcgtag acgctcatcg ccgcccggtt gcccatgttc gccgcgtcgc
     1261 ccttgcccgg ctcgttgctg tcgaacgagg gaccgttggc catcaccagc acctcaccgg
     1321 tgcggggatc catgacgatc acggcggccg cctgggcccc cgtgtcggcc aacgtcttgg
     1381 ccagccgctg ctcggccatc aactgcagag aactgtcgat ggtgaggccg aagttgtgcc
     1441 cgttctgcgc cgggatcagt acgttgtcgc cgagcgggat cttgccgttg ggcgaggagt
     1501 cgtagacctc tttgccgtcg gtgccacgca ggagcttctc gagcgagagt tccaggccgc
     1561 cggcggcctg gccctgctcg ttcacccagc cgatcaggtt ggccgccagg gtgccgttgg
     1621 ggtagcgccg ggtcggtgcg gtcacccgcg agagcccgag caggccggcc gccttcaact
     1681 cggcctgcaa ctgctcgaac gtgttggccg gcacccgctt ggcgatcacc ttccacgagc
     1741 tcttctggtc ggtcagctcc ggcaggtacg actcgggggt gttgcccagg tacttggcca
     1801 gcagttgcgc gatgcgctgc ggcgcggccg ccgcgatctc cttgtcgcgc gccgtcatcg
     1861 gctgtaggtc catcttgccg ttggtgcgga tcgccttcgg gtcggcgatc acgttgacgg
     1921 tctgctcggt gtaggccagc acggcaccgt agcggtcggt gatgtcgccg cgggccgcgg
     1981 gcagcaccag cgactcctgc atgcggtcgg cggcctcggt cgcgtaggcc ttggcgtcga
     2041 aggcctgcac ctgcagggcg cgtccggcgc acaggctgaa gaccatcgcg atcatcagca
     2101 gcagcacccg catgcgcttg accggttgcg caagcggcca acgcacccgg cccatggact
     2161 tcagccggcg ctgggtgctg ctcttgagcg tcatcgatcc gtcccgttct tcttgccggt
     2221 cttcttcttg gccgcctcgg ccttctgctg ctcgtccgcc ttcttcttcg ccgccgcttc
     2281 ggccttcttc gtctcgtcgg ccttcttctt ggcggccgcc tcggccttcg ccttctcttc
     2341 ggcggccttc ttcttcgcct cggcggcagc cttctgccgg gcggcgacct cagccaaccg
     2401 cttggcctcg gtgtcggccg cctgcttggt cagagccgcc agctgctcag gcgtgcggta
     2461 acggacgtcc ggcagctcgt agccgctgat ccgggtcggg tcgccgatca ccgtgccggc
     2521 cggcagcgac aggtagaccg ggtaggggtt gggcaccatg cccagcttgg tggcctcctc
     2581 ggccagccgc gtgctcgagc gggcctcggt gatctcggac tcgagttccg ccacccggta
     2641 gccgagctgg tcggccgcct tctgctggtt ctgcaccgcg aagccctgct cctgcaaggc
     2701 ggtgttgagc agcagcaggc ccaccatgcc gagggtgaga aacaccgcga gcgtgagcac
     2761 gaacggcagg ttcgccattc gccgctccgg gatcggcacc tgggtcagcc cccagcgccg
     2821 aggcttctcc tccgggcttc tgcggggtgc ttcgagtgct gtcatgccgc cacctctacg
     2881 atccgttcac cgacccgcag ccgcgccgag gcggctcgcg ggttgacctg cacttcgtcc
     2941 ggccccggac gctcggcgcc ccgggtcagc agcatcagtt gcgcacgcaa tccgtccggc
     3001 accgcgggca gaccgcgcgg ggcccggtca gtggccgcgt cgcgcagcgc gttcttcacc
     3061 agccggtcct cgccggagtg gtaggccagc accgcgagcc gtccacccgg tgcgagcgcg
     3121 gcgatggcgg cgggcagcag cccggccagg gcgaccaact cgtcgttcac ggcgatccgc
     3181 agcgcctgga aggtccgctt ggccgggtgc ccgcccgtgg cccgggctgc ggccgggatc
     3241 gcaccctcga tcacccgcac caggcgcccg gaggtggtga agggctcatc ggcacgctcc
     3301 gcgaccacgc gacgggcgat ccggtcggcg aagcgttcct cgccgaagcg tcgcaggatc
     3361 atgctcagtt cggccgcgga cgcctggttg accaggtcgg cggcggtcag cgtctgggtg
     3421 tcgtccatgc gcatgtcgag gggggcatcc acgctgtacg cgaaaccgcg ctcgcgacga
     3481 tcaatctgca tcgacgacaa ccccagatcg gcgaggatcg cctgcacccg cggccgtccg
     3541 gcctcggcca gcacctcggg cagttcgtcg tagcgggcct gatacagctg cacccggtca
     3601 ccgaagcgcg cgagacgttc gcccgccagc cgcaaggcgt gctggtcgcg gtcgatgcca
     3661 atcagggtgg ctcgggggca ggcttcgaga atcgcctccg catggccgcc caggccgagg
     3721 gtgcagtcga cgtagcggga gtcagcggcc tgcagcggca cactcagcag ttcgacgatg
     3781 cgcgcacaca tcaccggcac gtgcgccacg gcctgctgcc cgtccatctc gacccttcct
     3841 cgtttcgatg gcccatcgcg tgcgttgtgg tttcaatccg tgagtaccgc ctggcaccgg
     3901 ggaaggtgcg ccagggggcg gagcccctcg gattgaagcc gcaccgcacg cgacggtgtt
     3961 gcctgctcgg tgcgggtcac cccggtccga atacctcttc gtccatctgc gcgaaggcct
     4021 cttcctgcgc cgcggcgtag gaggcccagg cctccgcgtc ccacacctcg atgtggtcca
     4081 gggcaccgat cacgacgatc tcccgctgca ggcccgcata cgtgcgcagg tgctcgggaa
     4141 cggtgatccg gccctgcccg tcgggggtgg atgaatccgc tcccgaggcg aacatgcgct
     4201 gcagcgagcg caccttcgcc aggctggacg atcccgcgat catcttgtcg gcggccgcct
     4261 ggtaggcggc cggtgtgaag atcgccagac agcggtcggg ctgccgggtg atgacaagac
     4321 ccccgttcag ttcctcgcgg aacttggcgg ggaggaagaa gcggcctttg tcatcgagct
     4381 tgggggtgtg cgtgcccagg aacatcgggc ctccccttcc actctgtcct ccactgtgcg
     4441 ccactgtact ccactttgct ccaccgaggc acccctggag ggcactttcg ggcccggtaa
     4501 agtggccacc accgagagcc gagggggaac cgtggagcgc accgcagcga gccgggtgga
     4561 cgatgtgcag gcggtcgccc agtcgatccg cggtgctgtc tcgacggtga tcgagggcaa
     4621 gcccgaggcg atcgagctgg ccctggtggc gctgttctcg tccggtcacc tgctgatcga
     4681 ggacgtgccc ggcgtcggca aaaccatgct cgccaaggcg ctggcccgct cgatctcggg
     4741 caccgcgcag cgcattcagt tcacccccga cctgctgccc agcgacatca ccggggtgtc
     4801 gatcttcaac tcgcagcggc acgacttcga gttcaagccg gggggcgtct tcgccaacgt
     4861 ggtgatcggc gacgagatca accgcgcctc gccgaagacc cagtcggcgc tgctcgaggc
     4921 gatggaggag catcaggtca ccgtcgacgg caccacccac ccgctgcccg atccgttcct
     4981 ggtggtcgcc acccagaatc cgatcgagat ggagggcacc tatcccctgc ccgaggcgca
     5041 gcgggatcgg ttcatgctgc gcatctc
//
LOCUS       DAOSUG010000190         5056 bp    DNA     linear   ENV 10-JUN-2024
DEFINITION  MAG TPA_asm: Micropruina sp. isolate AMR_MDS_2195
            NODE_7316_length_5056_cov_4.382723, whole genome shotgun sequence.
ACCESSION   DAOSUG010000190 DAOSUG010000000
VERSION     DAOSUG010000190.1
DBLINK      BioProject: PRJNA925477
            BioSample: SAMN32864262
            Sequence Read Archive: SRR4236673
KEYWORDS    WGS; ENV; Metagenome Assembled Genome; MAG; Third Party Data; TPA;
            TPA:assembly.
SOURCE      Micropruina sp. (wastewater metagenome)
  ORGANISM  Micropruina sp.
            Bacteria; Bacillati; Actinomycetota; Actinomycetes;
            Propionibacteriales; Nocardioidaceae; Micropruina.
REFERENCE   1  (bases 1 to 5056)
  AUTHORS   Abdulkadir,N., Saraiva,J.P., Zhang,J., Stolte,S., Gillor,O.,
            Harms,H. and Rocha,U.
  TITLE     Genome-centric analyses of 165 metagenomes show that mobile genetic
            elements are crucial for the transmission of antimicrobial
            resistance genes to pathogens in activated sludge and wastewater
  JOURNAL   Microbiol Spectr 12 (3), e0291823 (2024)
   PUBMED   38289113
REFERENCE   2  (bases 1 to 5056)
  AUTHORS   Abdulkadir,N.
  TITLE     Direct Submission
  JOURNAL   Submitted (24-MAR-2023) Environmental Microbiology, Helmholtz
            Center for Environmental Research -UFZ, Permoserstr 15, Leipzig
            04318, Germany
COMMENT     The annotation was added by the NCBI Prokaryotic Genome Annotation
            Pipeline (PGAP). Information about PGAP can be found here:
            https://www.ncbi.nlm.nih.gov/genome/annotation_prok/
            
            ##Genome-Assembly-Data-START##
            Assembly Method        :: SPAdes v. 3.11.1
            Genome Representation  :: Full
            Expected Final Version :: Yes
            Genome Coverage        :: Not Applicable
            Sequencing Technology  :: Illumina MiSeq
            ##Genome-Assembly-Data-END##
            
            ##Genome-Annotation-Data-START##
            Annotation Provider               :: NCBI
            Annotation Date                   :: 05/04/2023 04:57:10
            Annotation Pipeline               :: NCBI Prokaryotic Genome
                                                 Annotation Pipeline (PGAP)
            Annotation Method                 :: Best-placed reference protein
                                                 set; GeneMarkS-2+
            Annotation Software revision      :: 6.5
            Features Annotated                :: Gene; CDS; rRNA; tRNA; ncRNA
            Genes (total)                     :: 2,776
            CDSs (total)                      :: 2,750
            Genes (coding)                    :: 2,714
            CDSs (with protein)               :: 2,714
            Genes (RNA)                       :: 26
            tRNAs                             :: 24
            ncRNAs                            :: 2
            Pseudo Genes (total)              :: 36
            CDSs (without protein)            :: 36
            Pseudo Genes (ambiguous residues) :: 0 of 36
            Pseudo Genes (frameshifted)       :: 2 of 36
            Pseudo Genes (incomplete)         :: 32 of 36
            Pseudo Genes (internal stop)      :: 2 of 36
            ##Genome-Annotation-Data-END##
FEATURES             Location/Qualifiers
     source          1..5056
                     /organism="Micropruina sp."
                     /mol_type="genomic DNA"
                     /submitter_seqid="NODE_7316_length_5056_cov_4.382723"
                     /isolate="AMR_MDS_2195"
                     /isolation_source="Activated sludge of municipal
                     wastewater treatment plants"
                     /db_xref="taxon:2737536"
                     /environmental_sample
                     /geo_loc_name="Canada"
                     /lat_lon="49.25 N 123.1 W"
                     /collection_date="2015-06-16"
                     /metagenome_source="wastewater metagenome"
                     /note="metagenomic"
     gene            complement(<1..942)
                     /locus_tag="PLF56_07370"
     CDS             complement(<1..942)
                     /locus_tag="PLF56_07370"
                     /inference="COORDINATES: protein motif:HMM:NF024180.2"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="CHAT domain-containing protein"
                     /protein_id="HOQ53417.1"
                     /translation="MAALDYLDFDLEVEAGATPDVYKVSVLSSPRGEASGQMTFPFNS
                     DALENVILKLGRTRSGVRAIGSPTQDLAQKFGTTLYDALFAGEVGTCFRRSLDEAGKQ
                     GKGLRIRLRLEDAPQLAEVPWEYLYPSGLRQFLVLSTRTPIVRYLELPQPVAALRVTP
                     PLQVLVVVSSPSNLATLNVEAEVQRIRGALASLERAGQVRVTVIPAPAADGARTHASL
                     MDVRRALRSGGFHVLHFIGHGGFVDATGEGMLAFEDENLLAHMVSGTTLATTISGFDS
                     LRLVLLNACEGARQSPADPLGGVAQSLVAQGVPAVVAM"
     gene            complement(1022..2521)
                     /locus_tag="PLF56_07375"
     CDS             complement(1022..2521)
                     /locus_tag="PLF56_07375"
                     /inference="COORDINATES: ab initio
                     prediction:GeneMarkS-2+"
                     /note="Derived by automated computational analysis using
                     gene prediction method: GeneMarkS-2+."
                     /codon_start=1
                     /transl_table=11
                     /product="hypothetical protein"
                     /protein_id="HOQ53418.1"
                     /translation="MPTLDGTSRQVTLGQGITLRAPGLRGTAEAHNVGPGSDTRAPDV
                     DGSGPALDEALTREGFLRLRVITLDVAPVPQAPGSDIRSPVADVDGLEVNVPDLGDDV
                     AQVLLAVDENGVVSWNFPVDSSDRLAPTTRGAGDSVRFIVPAFATEPGPADTATNRGV
                     FGLIGRKVLELVALPLAELVVPPLAGAAARLWESKSRIARARTFTPANYTDPGVPSLD
                     EAEWRRLAAGRSLWFVHGTFVNTPSAFQALAPDVLAALSDAYGGRVAALDHHTLGLSP
                     IENADLVRELVPEGVQLEVDIVCHSRGGLVSRALAGQGTDAVFDVRRIVHVASANHGT
                     ALATPSNLVPLIDRITTIVNLMPDGPAALVEATLTSVLVVVKIIAKYGLTGIPGLAAM
                     DSAGEFLSTFNHTELPVEQYAVAADYSPSGGWRAMALKNVENVVVDRVFGTVANDLVV
                     PTAGVYEGEDAMAIPENRRLVLPPERGVYHGSFFGQPDVAKQIAGWLTG"
     gene            complement(2521..4707)
                     /locus_tag="PLF56_07380"
     CDS             complement(2521..4707)
                     /locus_tag="PLF56_07380"
                     /inference="COORDINATES: protein motif:HMM:NF024180.2"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="CHAT domain-containing protein"
                     /protein_id="HOQ53419.1"
                     /translation="MDPVLVLDAAMTLAAARSALSLATPRITVVVRRLDPRASGAILW
                     YPLSEHDLQAVVRGDLDPAMPLGVSLDLHEHGASQVRQLTHPDVAGGFWTGLVVDGSR
                     AVGWMSADPHDTRGPSRGIDFGAGIAPPAPRPSAPGLAPVVADQAGPGDGAGAEGAPD
                     AGEASSAEGGNSTGGGASAGAGTGTEGGTGAGVGTDTIGEPIPAVPAYPRVDVPDTVG
                     SGLAFTVVVGLSKQAQPGVQGAVMNIAADHQPLIVQIKAENFTLAGVRFELPFDPADV
                     AAQTVTIPVTAPVVHQNWRGRIEIEYSQDGLLVGTAWRELVVSPQAPADAPPSTSGGE
                     VAVELDHSVPIDLTVSISRGEADGTFLWTFTTPHPVPLPDHQVITHLEDDTAETFALK
                     KVREMARVDGSDAAELKVNGVARAVAAVMPLEFWTVLAPVWRLAKDDDRLPTLLLVSQ
                     ESLVPWELATTDADYIVDRTLVDQAAPQLLGAQLILGRWRPAGPETPSGMRRPSTTPA
                     ETIAVGGIAVVVGEFGPSSGMRPLKMAVEEGQQLTAAYQPSFWVKGTLSDVSSLLKGT
                     YTDRGAPISAQVLHVASHGEVDPDSPINTGVILSDSAVRIDESIVVGSDFTRTAAPLV
                     FLNCCQLATETGGSLVEGGLAAAFLQAGARAFVAPLWSVDDTIAKQTALSFYADAFAE
                     ARPVGEVMRRLRARFTTDYPARNQTTPLAYAYYGHPGLILSRQEPH"
BASE COUNT          763 a         1742 c         1813 g          738 t
ORIGIN      
        1 catcgccacc accgccggga ccccctgggc gaccaggctc tgcgccaccc cgcccagtgg
       61 gtcggcgggc gactgccgcg cgccctcgca cgcgttcagc agcacgagcc gcaacgagtc
      121 gaagccgctg atcgtggtgg ccagcgtggt gcccgacacc atgtgggcca agaggttctc
      181 atcctcgaac gcgagcatgc cctcgccggt ggcgtccacg aagcccccgt gcccgatgaa
      241 atgcagcacg tgaaacccgc ctgatcgcag ggctcggcgc acgtccatca ggctggcgtg
      301 ggtacgcgct ccgtcggcgg ccggagccgg aatcaccgtc acccgcacct ggcccgcccg
      361 ttcgagcgag gccagcgcgc cgcggatgcg ttgcacctcg gcctcgacgt tcagcgtcgc
      421 caggttggac ggcgacgaca ccaccaccag cacctgcagc ggcggggtca cccgcagcgc
      481 ggccaccggc tgcggcaact ccagataccg gacgatcggc gtgcgggtcg acagcaccag
      541 aaactgccgc agccccgacg ggtagaggta ctcccacggc acctcggcca actgtggggc
      601 gtcctcgagc cgcagccgga tccgcagccc ctttccctgc ttgcccgcct cgtcgagact
      661 gcgtcgaaaa caggtgccga cctcgccggc gaacagcgcg tcgtacaggg tggtgccgaa
      721 cttctgcgcc aggtcctggg tgggcgaccc gatcgcccgg acgcccgagc gggtgcggcc
      781 cagcttgagg atcacgttct cgagggcgtc ggagttgaag gggaacgtca tctgcccgga
      841 cgcctcgccg cgcggcgagg aaagcaccga gaccttgtag acgtcaggcg tcgccccggc
      901 ctcgacctcg aggtcgaaat ccagatagtc gagcgcggcc acgaaccccc tccgatcctc
      961 gtgcgacgtc gttcgtgcga cgtccttcgt gcgacgtcct tggtgcgaca gtgtcgtccg
     1021 gctaccccgt cagccagccg gcgatctgct tcgcgacgtc gggctggccg aagaagctgc
     1081 cgtgatacac cccgcgctcc gggggcagca caagccgccg gttctcgggt atcgccatcg
     1141 cgtcctcgcc ctcgtagacg cccgccgtcg gcaccaccag gtcgttggcg acggtgccga
     1201 acacccggtc gaccaccacg ttctcgacgt tcttcaacgc catcgcccgc cagcctcccg
     1261 acgggctgta gtcggccgcc accgcgtact gctcgacggg cagttcggtg tggttgaaag
     1321 tgctcaggaa ctcgccggca ctgtccatgg cggccagccc cggaatgccg gtcagcccgt
     1381 acttggcgat gatcttgacc accaccagca ccgaggtgag cgtcgcctcg accagggcgg
     1441 ccggcccgtc cggcatcagg ttgacgatgg tggtgatccg gtcgatcagc ggcaccagat
     1501 tggacggggt ggcgagtgcg gtgccgtggt tggcgctggc cacatggacg atgcggcgca
     1561 cgtcgaacac cgcgtcggtg ccctgcccgg ccagcgcccg gctgaccagg ccgccccggc
     1621 tgtggcagac gatgtccacc tcgagctgca caccctcggg caccagttcg cgcaccaggt
     1681 ccgcattctc gatcggcgac aatcccaggg tgtgatgatc gagcgccgcg acccgtcccc
     1741 cgtaggcgtc gctgagcgcg gccagcacgt ccggcgccag ggcctggaac gcgctgggcg
     1801 tgttcacgaa ggtgccgtgc acgaaccaca ggctgcggcc cgccgccagt cgccgccatt
     1861 cggcctcgtc caggctcggc acgcccgggt cggtgtagtt ggcgggggtg aaggtacgag
     1921 cccgggcgat tcgcgatttg gactcccaca gccgggcggc tgccccggcc agcggcggca
     1981 ccaccagttc ggccagcggg agggcgacca actcgagcac cttgcgtccg atcaggccga
     2041 agacccctct gttggtggcc gtatcggccg ggcccggttc ggtggcgaac gcgggcacga
     2101 tgaaacgcac ggaatctccc gcgccgcggg tggtcggtgc cagccgatcg gacgaatcga
     2161 ccggaaagtt ccagctgacc acgccgttct cgtccaccgc cagcaacacc tgagcgacgt
     2221 cgtcgcccag gtcaggcacg ttgacctcaa gaccgtcgac gtcggcgacc ggcgagcgga
     2281 tgtcgctgcc cggcgcctgc ggcaccggcg ccacgtccag ggtgatcacc cgcagccgca
     2341 ggaacccctc gcgggtcagg gcctcgtcca gggcgggacc ggaaccgtcg acgtcggggg
     2401 cgcgggtgtc cgacccaggc cccacgttgt gcgcctcggc ggtgccgcgc aaccccggtg
     2461 cgcgcagggt gatgccctgg ccgagagtga cctgcctgct ggttccgtcg agcgtgggca
     2521 tcagtgcggc tcctgcctgc tgagaatgag tccgggatgg ccgtagtagg cgtaagccaa
     2581 cggggtggtc tggttgcggg ccgggtagtc ggtggtgaag cgggcgcgca gccggcgcat
     2641 cacctcaccg accggccggg cctcggcgaa ggcgtcggcg tagaagctca acgcggtctg
     2701 tttggcgatg gtgtcgtcca ccgaccacag gggcgcgacg aaggcgcggg cgccggcctg
     2761 cagaaaggcg gcggccaatc cgccctcgac cagcgagccg ccggtctcgg tggccagttg
     2821 gcagcagttg agaaacacca gcggggccgc ggtgcgggtg aagtccgaac ccaccacgat
     2881 cgactcgtcg atgcgcaccg ccgaatcgga caggatcacc ccggtgttga tcggagaatc
     2941 cgggtcgacc tcaccgtgcg aggcgacgtg cagcacctgg gcgctgatcg gcgcgccacg
     3001 atcggtgtag gtgcccttga gcagcgagga cacgtcactc agcgtgccct tcacccagaa
     3061 ggacggctgg taggcggcgg tcagttgctg gccctcctcg acggccatct tgagcggacg
     3121 catgcctgag ctggggccga actcgccgac cacgacggcg atcccgccga cggcgatggt
     3181 ttcggccggt gtggtgctcg gacggcgcat gcccgagggg gtttcgggac cggccgggcg
     3241 ccaccgtccg aggataagtt gggcgccgag cagttgtggg gcggcctgat cgaccagggt
     3301 gcgatccacg atgtagtcgg catcggtggt cgccagttcc cagggaacca gtgactcctg
     3361 cgacaccaac agcagggtgg gcagccggtc gtcgtccttg gcgaggcgcc agacgggggc
     3421 cagcaccgtc cagaactcga gcggcatcac cgcggccacc gcacgggcga cgccgttgac
     3481 cttgagttcg gccgcgtccg acccgtccac ccgggccatc tcgcgcacct tcttcagggc
     3541 gaaggtctcg gcggtgtcgt cctcgaggtg ggtgatcacc tgatggtcgg gcagcggtac
     3601 ggggtggggg gtggtgaacg tccacagaaa ggttccgtcg gcctcgccgc ggctgatcga
     3661 gacggtcagg tcgatcggca cgctgtggtc gagttcgacg gcgacctcgc ccccggacgt
     3721 ggacggcggc gcgtccgccg gtgcctgcgg gctgaccacg agttcgcgcc aggcggtgcc
     3781 gaccagcagc ccgtcctggc tgtactcgat ctcgatccgg cctcgccagt tctggtgcac
     3841 gacgggggcg gtcacgggga tcgtcaccgt ttgggccgcc acgtctgccg ggtcgaaggg
     3901 caactcgaac cgcacgccgg cgagggtgaa gttctcggcc ttgatctgca cgatcagggg
     3961 ttggtggtcc gcggcgatgt tcatcacggc accttggacg ccgggttggg cctgcttgga
     4021 caggccgact accaccgtga acgccagacc cgagccgacc gtgtcgggca catccacgcg
     4081 gggataggcc gggacggcag ggatcggttc gccaatcgtg tcggtgccca cgcccgcacc
     4141 ggtgcctcct tccgtaccgg tgcccgctcc tgcgctggcg cctcctcccg tactgttgcc
     4201 gccctccgcg ctggaagcct ctcccgcgtc gggggcgccc tccgcgccgg cgccgtcccc
     4261 gggtccggcc tggtcggcca ccaccggtgc cagacccggt gcggatggcc gcggagcggg
     4321 cggtgcgatg ccggcgccga agtcgatgcc gcggctcggg ccgcgggtgt cgtgggggtc
     4381 ggcggacatc caaccgaccg cgcgagagcc gtccacgacc aggccggtcc agaaaccgcc
     4441 ggcgacatcg ggatgggtga gttggcgaac ctgcgacgcg ccgtgttcgt gcaggtcgag
     4501 tgagacgccc agcggcatgg cggggtcgag gtcgccgcgg accaccgcct gcagatcgtg
     4561 ctcgctcagg gggtaccaca ggatcgcacc ggaggcgcgg gggtcgagac ggcgcaccac
     4621 gaccgtgatg cgcggggtgg ctagcgacaa ggcgctgcgc gctgcggcga gcgtcatggc
     4681 ggcatcgagc acgagcacgg gatccatcag cgactcctct cgagccaaga ggggcgggcg
     4741 gctcacggtg atacggcgca acagtgatac gcagcgtaga accgcgcgtc gccgcgcgga
     4801 gaagcgtgcc cgaacagttt gcgccgtaca ggtggcagtt ttgccacctt gcgcgatcga
     4861 cctccgtcgc caaccgggat gtcgtagcgg tgcctgggcg ggcgatgcgt gcggcagctg
     4921 atatgcccgg atccaccgaa ggcgccgtgg cggtcgggac ccggcgggtg ggtgggcaag
     4981 ccggtcgcgc atgacgggcc ggtgagcagt gcgtggcaac ggttggtgag cagtgcgtgg
     5041 caacggttgg tgagca
//
LOCUS       DAOSUG010000191         4998 bp    DNA     linear   ENV 10-JUN-2024
DEFINITION  MAG TPA_asm: Micropruina sp. isolate AMR_MDS_2195
            NODE_7390_length_4998_cov_4.366984, whole genome shotgun sequence.
ACCESSION   DAOSUG010000191 DAOSUG010000000
VERSION     DAOSUG010000191.1
DBLINK      BioProject: PRJNA925477
            BioSample: SAMN32864262
            Sequence Read Archive: SRR4236673
KEYWORDS    WGS; ENV; Metagenome Assembled Genome; MAG; Third Party Data; TPA;
            TPA:assembly.
SOURCE      Micropruina sp. (wastewater metagenome)
  ORGANISM  Micropruina sp.
            Bacteria; Bacillati; Actinomycetota; Actinomycetes;
            Propionibacteriales; Nocardioidaceae; Micropruina.
REFERENCE   1  (bases 1 to 4998)
  AUTHORS   Abdulkadir,N., Saraiva,J.P., Zhang,J., Stolte,S., Gillor,O.,
            Harms,H. and Rocha,U.
  TITLE     Genome-centric analyses of 165 metagenomes show that mobile genetic
            elements are crucial for the transmission of antimicrobial
            resistance genes to pathogens in activated sludge and wastewater
  JOURNAL   Microbiol Spectr 12 (3), e0291823 (2024)
   PUBMED   38289113
REFERENCE   2  (bases 1 to 4998)
  AUTHORS   Abdulkadir,N.
  TITLE     Direct Submission
  JOURNAL   Submitted (24-MAR-2023) Environmental Microbiology, Helmholtz
            Center for Environmental Research -UFZ, Permoserstr 15, Leipzig
            04318, Germany
COMMENT     The annotation was added by the NCBI Prokaryotic Genome Annotation
            Pipeline (PGAP). Information about PGAP can be found here:
            https://www.ncbi.nlm.nih.gov/genome/annotation_prok/
            
            ##Genome-Assembly-Data-START##
            Assembly Method        :: SPAdes v. 3.11.1
            Genome Representation  :: Full
            Expected Final Version :: Yes
            Genome Coverage        :: Not Applicable
            Sequencing Technology  :: Illumina MiSeq
            ##Genome-Assembly-Data-END##
            
            ##Genome-Annotation-Data-START##
            Annotation Provider               :: NCBI
            Annotation Date                   :: 05/04/2023 04:57:10
            Annotation Pipeline               :: NCBI Prokaryotic Genome
                                                 Annotation Pipeline (PGAP)
            Annotation Method                 :: Best-placed reference protein
                                                 set; GeneMarkS-2+
            Annotation Software revision      :: 6.5
            Features Annotated                :: Gene; CDS; rRNA; tRNA; ncRNA
            Genes (total)                     :: 2,776
            CDSs (total)                      :: 2,750
            Genes (coding)                    :: 2,714
            CDSs (with protein)               :: 2,714
            Genes (RNA)                       :: 26
            tRNAs                             :: 24
            ncRNAs                            :: 2
            Pseudo Genes (total)              :: 36
            CDSs (without protein)            :: 36
            Pseudo Genes (ambiguous residues) :: 0 of 36
            Pseudo Genes (frameshifted)       :: 2 of 36
            Pseudo Genes (incomplete)         :: 32 of 36
            Pseudo Genes (internal stop)      :: 2 of 36
            ##Genome-Annotation-Data-END##
FEATURES             Location/Qualifiers
     source          1..4998
                     /organism="Micropruina sp."
                     /mol_type="genomic DNA"
                     /submitter_seqid="NODE_7390_length_4998_cov_4.366984"
                     /isolate="AMR_MDS_2195"
                     /isolation_source="Activated sludge of municipal
                     wastewater treatment plants"
                     /db_xref="taxon:2737536"
                     /environmental_sample
                     /geo_loc_name="Canada"
                     /lat_lon="49.25 N 123.1 W"
                     /collection_date="2015-06-16"
                     /metagenome_source="wastewater metagenome"
                     /note="metagenomic"
     gene            <1..313
                     /locus_tag="PLF56_07385"
     CDS             <1..313
                     /locus_tag="PLF56_07385"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_013160008.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=2
                     /transl_table=11
                     /product="sensor histidine kinase"
                     /protein_id="HOQ53420.1"
                     /translation="TIHVLLEGDRLRISDQGPGIADDELPHVFDRFWRSPSARNAPGS
                     GLGLSIVAQTIKSHGGWVKAGHSAEGGAEFIIRLPGQATPPGEEEDEDESTVLLPKIK
                     S"
     gene            complement(980..1174)
                     /locus_tag="PLF56_07390"
     CDS             complement(980..1174)
                     /locus_tag="PLF56_07390"
                     /inference="COORDINATES: ab initio
                     prediction:GeneMarkS-2+"
                     /note="Derived by automated computational analysis using
                     gene prediction method: GeneMarkS-2+."
                     /codon_start=1
                     /transl_table=11
                     /product="hypothetical protein"
                     /protein_id="HOQ53421.1"
                     /translation="MAETRKYNPATSAVGDAIRAALGEDVRPVQPGEKPQPPDGEPAS
                     PSAPPAVTVKTFTFGVKKAN"
     gene            1286..2689
                     /locus_tag="PLF56_07395"
     CDS             1286..2689
                     /locus_tag="PLF56_07395"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_015071417.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_function: GO:0022857 - transmembrane transporter
                     activity [Evidence IEA];
                     GO_process: GO:0055085 - transmembrane transport [Evidence
                     IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="MFS transporter"
                     /protein_id="HOQ53422.1"
                     /translation="MNRRRFHQLLLNTFLANVTTGFLWFALVFWVFLETRSVLVTSFM
                     GGAYMLLVAVVGVPFGTWVDRTRKRQVMLVAQVFTTVAFALALVLFLSTPQEQLLSLS
                     GWQLYAFVGLSLLGAVVESARNIALSTCVTLMIDESERAQANGLVGMVGGLGFAVTSV
                     FSGLAVGRLGITVTLVIAVALTVVSLVHLLFVQIPEPQIVHVDGAPRAIDFAGAFRAI
                     REVPGLTGLVLFACFNNLLGGVFMALLDPYGLTLVSVEVWGVLWGVLSFGFIIGAGWV
                     SRKGLGAQPLRALLLANVGMWLVAMSFTIRESIWLLAAGTLVYLALIPVAEAAEQTVL
                     QKVVPFAKQGRVFGLAQTIEVGAAPITAFVIGPVAQFWLIPYADSDAGRAQLGWLLGE
                     GQARGIALVFLLAGLGGLALTLFAFTTRSYRWLAARYSEPSADDAGPGVQPDSTVTDE
                     QPLDPAEPTAGGDTRPP"
     gene            complement(2697..3335)
                     /locus_tag="PLF56_07400"
     CDS             complement(2697..3335)
                     /locus_tag="PLF56_07400"
                     /EC_number="2.1.-.-"
                     /inference="COORDINATES: protein motif:HMM:NF024880.2"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_function: GO:0008168 - methyltransferase activity
                     [Evidence IEA];
                     GO_function: GO:1904047 - S-adenosyl-L-methionine binding
                     [Evidence IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="class I SAM-dependent methyltransferase"
                     /protein_id="HOQ53423.1"
                     /translation="MNATIESSGIGDGGSYDGRAAAYDRLVGSQLYNRLAWGADPADY
                     AAFARRAVASASGPVLDVAAGTATATAEAYRASDRTIVLTDRSTDMLRVAAQRIARDA
                     ELRPGIRFVQADAFNLPFQPGGFDTVLCLGFLHLIDDPGGFVDGLRAQARDGGRVFVS
                     SLVAGRRIGDGYLRLLHRLGEVAAPRTADDLADLFDTRVLRHGSMAYLELPC"
     gene            complement(3492..4952)
                     /gene="cycA"
                     /locus_tag="PLF56_07405"
                     /gene_synonym="dagA"
     CDS             complement(3492..4952)
                     /gene="cycA"
                     /locus_tag="PLF56_07405"
                     /gene_synonym="dagA"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_013161883.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_component: GO:0016020 - membrane [Evidence IEA];
                     GO_process: GO:0006865 - amino acid transport [Evidence
                     IEA];
                     GO_process: GO:0055085 - transmembrane transport [Evidence
                     IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="D-serine/D-alanine/glycine transporter"
                     /protein_id="HOQ53424.1"
                     /translation="MTNLTAKPRPSQTPADGQPDESHHLARALGNRHLQMIAIGGAIG
                     TGLFMGSGKTISLAGPSIVLVYAIIGAFLFFVMRAMGELLLSNLNYKSFRDIAEDVLG
                     PWAGFFAGWTYWFCWIVIGVADLTAVTAYVSYWFPDLPRWIAATCLVLLLLALNLVTV
                     KLFGEIEFWFALIKIVAIVALIVVAIVMVAVGFVSPEGHQASVANLWNGGGFFPTGAG
                     GFLAGFQIAFFAFLGIELVGTAAAETKDPARTLPKAINAIPIRVGLFYICALAAIMAV
                     TPWQLINPAVSPFVSMFGLAGFGLAASAINFVVLTSAASSANSGIYSTSRMLFGLALD
                     RKAPRQFGRLSKFKVPANGLIFSCCCLLPGLILLYTTDSIIAAFTLATTVASVLFIFV
                     WGLIVASYLVYRRRNPEKHEASVYKMPAGVVMSWAVFAFFGVMIGILAMEPDTRMALA
                     VTPVWFIALGIAYWMHLRRSRAHAIVEKPEAIKVAE"
BASE COUNT          814 a         1661 c         1714 g          809 t
ORIGIN      
        1 aacgattcac gttctgctcg aaggtgaccg actgcgcatc tcggatcagg gccccggcat
       61 agccgatgac gaactgccgc acgtgttcga ccggttctgg cgctcgccct cggcccgcaa
      121 cgccccgggc tcggggctgg gcctgtcgat cgtcgcgcag acgatcaaat cgcacggcgg
      181 ttgggtcaag gccgggcatt ccgcagaagg cggcgccgag ttcatcattc ggctaccggg
      241 tcaggccaca ccgcccggcg aagaggagga cgaggacgaa tcgacggtgc tgctgccgaa
      301 gatcaagagc tgagcggggc ttgatcggag ccacgatcgg ttggctgggc ttgtcgaagc
      361 ccctaccggc gagcatctcg acacgctcga ccaacgactg atcgggcgtg tccctgatcc
      421 tggatccagc gaacgggtcc gcgtcgaggt tgcgggcaga gctttcggca cggtcgccac
      481 gggcactgcc tgaagtccct gcaccagtgc agcgggtcgc gcctgcgcgc gccgcgaggt
      541 ctgccactgg tgcaggcttt tcaggcggcg gccagacgcg ccggctgttg gtcaggtggg
      601 cgagtcacca aggaacccac tcaatcacca ggtcccgggt aagccccaag gcgcccggat
      661 ggggccgcgc agtgggcgaa gatcggtgga tttgggctta gccggactta gcccctgcgt
      721 cggccacact caccagacgc ccggtatgcc ttcgcgcgga ccgtcttgcc aggccaccgg
      781 tttggtgccg cccgcgacgg cgcccatcgg tggatccagg ctaaggcagg ctaaggcggg
      841 ctaaaggcac accctgtcgg tccatccgaa ggcctccacc tgggtccgaa tttcggcgcc
      901 ggacctcgtg accagtggca cgcatacctg gtgggtacgc gcgggcagcg ctggagaaac
      961 accgctggtg aaagcgccgt cagttggcct tcttcacgcc gaacgtgaac gtcttgacag
     1021 tgaccgcggg cggcgccgat ggcgacgcag gctcaccgtc gggtggctgc ggcttctcgc
     1081 cgggctgaac cggccggacg tcctcgccca gcgcagcacg gatcgcgtcg cccaccgcgc
     1141 tggtcgccgg gttgtacttg cgggtctcgg ccatggccgg attctactgg ttgactgctg
     1201 tggccagacc ggcggttgac tgtccgtatg ctgtgcggtt tatgtcagcg gcgtccggca
     1261 gtcttgccgc aggacaggag gtgcgatgaa tcgccgacgt tttcaccagc tgctgctgaa
     1321 cacgtttctg gcgaacgtga ccaccggctt tctgtggttc gcgctcgtct tctgggtgtt
     1381 tctcgagacc cgttcggtgc tggtcacctc gttcatgggc ggcgcgtaca tgctgctggt
     1441 cgcggtggtc ggggtgccgt tcggcacctg ggtcgaccgc acccgcaagc gacaggtgat
     1501 gctggtcgcc caggttttca ccacggtcgc cttcgcgctg gccctggtgc tgtttctcag
     1561 cacaccccag gagcaactgc tcagcctgag cggctggcaa ctctacgcgt tcgtcgggct
     1621 cagcctgctc ggtgcggtcg tcgaatcggc ccgcaacatc gcgctctcga cctgtgtgac
     1681 gttgatgatc gacgagtccg aacgggccca ggcgaacggg ctggtgggca tggtcggcgg
     1741 tctcggtttc gcagtcacct cggtgttctc gggcctggcc gtcggccggc tgggcatcac
     1801 cgtcaccctg gtcatcgccg tcgcgctcac cgtggtgtcg ctggtgcacc tgctgttcgt
     1861 acagataccc gaaccacaga tcgtccatgt cgacggtgca ccccgagcca tcgactttgc
     1921 gggcgccttc cgcgcgatcc gtgaggtgcc cggcctcacc ggcctggtgc tgttcgcgtg
     1981 cttcaacaac ctgctgggcg gggtgttcat ggctctgctc gacccgtacg gcctcaccct
     2041 ggtctcggtc gaggtctggg gcgtgctgtg gggcgtgctc agtttcggct tcatcatcgg
     2101 cgccgggtgg gtctctcgca aaggcctggg tgcacaaccc ctgcgcgcgc tgctgctggc
     2161 caatgtgggc atgtggctgg tggcgatgag cttcacgatc cgcgagagca tctggctgct
     2221 ggcggccggc accctggtct acctggcact cattccggtc gccgaggccg ccgagcagac
     2281 cgtgttgcag aaggtggtgc cgttcgccaa acagggtcgg gtgttcgggc tggcccaaac
     2341 gatcgaggtg ggcgccgcgc cgatcaccgc cttcgtgatc ggcccggtgg cccagttctg
     2401 gctgatcccc tacgccgact cggacgcggg acgcgcccag ctcggctggt tgctgggcga
     2461 gggtcaggct cggggcatcg ctttggtctt cctgctggcc gggctgggcg gcctggccct
     2521 taccctgttc gcgttcacca cccgttcgta ccgctggctg gcagcgcgct acagcgaacc
     2581 gagcgcggac gatgcgggcc ccggcgtcca gccagacagt acggtcaccg acgagcagcc
     2641 gctcgacccg gccgaaccca ccgcaggcgg cgacacccgc ccaccatgac ccgtggtcag
     2701 cagggcagtt cgaggtaggc cattgaaccg tgccgcaaca cccgggtgtc gaacaggtcg
     2761 gccaggtcgt cggccgtccg cggtgcagcc acctcgccca gccggtgcag cagccgcagg
     2821 tagccgtcac cgatccgacg gccggccacc agcgagctga cgaacacccg tccgccatcc
     2881 cgagcctgcg cccgcaggcc gtcgacgaac ccgcccgggt cgtcgatcag gtgcagaaag
     2941 ccgaggcaga gcactgtgtc gaagccgccg ggctggaacg gcaggttgaa cgcgtccgcc
     3001 tgtacgaacc tgatgccggg acgcagttcc gcatcacgcg cgatccgctg agccgccacc
     3061 cgcagcatgt cggtcgaccg gtcggtcagc actatcgtcc ggtcgctggc tcggtaggcc
     3121 tcggcggttg cggtggccgt accggcggcc acgtccagca ccggtccgga ggcggacgcc
     3181 accgcccgcc gcgcgaaggc ggcgtagtcc gccgggtcag cgccccaggc cagccggttg
     3241 tacaactgcg agccgaccag gcggtcgtag gcggctgcgc ggccgtcgta gctaccgccg
     3301 tcgccgatgc cggacgactc gatggtcgcg ttcatttctc tcctgcctga ccgggtgccg
     3361 ttcgaccgta cgccctctcc cccgaccggc cgggcgcgct gacacagagg ggccccgccg
     3421 acgcgcaaca acagcagggc cggggaccgt ccaggctccc cgggcctgcc cgactcagcg
     3481 ggcgtccgcg ctcactcggc gaccttgatc gcctccggtt tttccacaat cgcgtgggcc
     3541 cggctgcgcc gcaggtgcat ccagtaggcg atacccaggg cgatgaacca aaccggggtg
     3601 acggccagcg ccatgcgggt gtcgggctcc atggccagaa tgccgatcat tacgccgaag
     3661 aacgcgaaca ccgcccagct catcacgaca cccgccggca tcttgtacac cgacgcctca
     3721 tgcttctcgg ggttgcggcg gcggtacacc aggtacgagg ccacgatgag tccccacacg
     3781 aagatgaaca gcaccgaggc caccgtggtc gccagggtga aggcggcgat gatcgagtcg
     3841 gtggtgtaca gcagaatcag gccgggcagc aggcagcagc acgagaagat caggccgttg
     3901 gcgggcacct tgaacttcga caacctgccg aactgacggg gggccttgcg gtccagggcc
     3961 aggccgaaca acatgcgcga ggtggaatag atgcccgagt tggccgacga ggcggccgag
     4021 gtcagcacca cgaagttgat cgccgaagcc gccaggccga agccggccag gccgaacatc
     4081 gacacgaacg ggctgaccgc ggggttgatc agctgccagg gcgtgacggc catgatggca
     4141 gccagtgcgc agatgtagaa caggcccacc cggatcggga tcgcattgat ggccttcggc
     4201 agggtgcggg ccgggtcctt ggtctcggcg gctgcggtgc cgaccagttc gatgccgaga
     4261 aaggcgaaga aggcgatctg gaagccggct agaaagccgc ccgccccggt cggaaagaag
     4321 ccgccgccgt tccacaggtt cgccacggac gcctgatgcc cctcgggcga gacgaagccg
     4381 accgcgacca tcacgatcgc gaccacgatc agggcgacga tcgccacgat cttgatcagc
     4441 gcgaaccaga actcgatctc gccgaacagc ttcacggtga ccaggttcag ggcgagcagc
     4501 agcagcacca ggcaggtggc cgcgatccaa cggggcaggt cggggaacca gtacgacacg
     4561 taggcggtca ccgcggtcag gtcggcgacg ccgatcacga tccagcagaa ccagtacgtc
     4621 cagcccgcga agaagcccgc ccagggcccg agcacgtcct cggcgatgtc gcggaacgac
     4681 ttgtagttga ggttgctgag cagcagctcg cccatggcgc gcatcacgaa gaacagaaag
     4741 gcgccgatga tcgcgtacac caacacgatg gacggtccgg cgagcgagat ggtcttgccg
     4801 gaacccatga acaggccggt gccgatcgcg ccgccgatgg cgatcatctg caggtggcgg
     4861 ttgcccagcg cgcgggcgag atggtgcgac tcgtcaggct ggccgtcggc gggggtctgc
     4921 gacggacgtg gtttggcggt caggttggtc atgaaaactc ccttgtcttc atcaggggga
     4981 aatggcggag tcacggcg
//
LOCUS       DAOSUG010000192         4968 bp    DNA     linear   ENV 10-JUN-2024
DEFINITION  MAG TPA_asm: Micropruina sp. isolate AMR_MDS_2195
            NODE_7434_length_4968_cov_3.843069, whole genome shotgun sequence.
ACCESSION   DAOSUG010000192 DAOSUG010000000
VERSION     DAOSUG010000192.1
DBLINK      BioProject: PRJNA925477
            BioSample: SAMN32864262
            Sequence Read Archive: SRR4236673
KEYWORDS    WGS; ENV; Metagenome Assembled Genome; MAG; Third Party Data; TPA;
            TPA:assembly.
SOURCE      Micropruina sp. (wastewater metagenome)
  ORGANISM  Micropruina sp.
            Bacteria; Bacillati; Actinomycetota; Actinomycetes;
            Propionibacteriales; Nocardioidaceae; Micropruina.
REFERENCE   1  (bases 1 to 4968)
  AUTHORS   Abdulkadir,N., Saraiva,J.P., Zhang,J., Stolte,S., Gillor,O.,
            Harms,H. and Rocha,U.
  TITLE     Genome-centric analyses of 165 metagenomes show that mobile genetic
            elements are crucial for the transmission of antimicrobial
            resistance genes to pathogens in activated sludge and wastewater
  JOURNAL   Microbiol Spectr 12 (3), e0291823 (2024)
   PUBMED   38289113
REFERENCE   2  (bases 1 to 4968)
  AUTHORS   Abdulkadir,N.
  TITLE     Direct Submission
  JOURNAL   Submitted (24-MAR-2023) Environmental Microbiology, Helmholtz
            Center for Environmental Research -UFZ, Permoserstr 15, Leipzig
            04318, Germany
COMMENT     The annotation was added by the NCBI Prokaryotic Genome Annotation
            Pipeline (PGAP). Information about PGAP can be found here:
            https://www.ncbi.nlm.nih.gov/genome/annotation_prok/
            
            ##Genome-Assembly-Data-START##
            Assembly Method        :: SPAdes v. 3.11.1
            Genome Representation  :: Full
            Expected Final Version :: Yes
            Genome Coverage        :: Not Applicable
            Sequencing Technology  :: Illumina MiSeq
            ##Genome-Assembly-Data-END##
            
            ##Genome-Annotation-Data-START##
            Annotation Provider               :: NCBI
            Annotation Date                   :: 05/04/2023 04:57:10
            Annotation Pipeline               :: NCBI Prokaryotic Genome
                                                 Annotation Pipeline (PGAP)
            Annotation Method                 :: Best-placed reference protein
                                                 set; GeneMarkS-2+
            Annotation Software revision      :: 6.5
            Features Annotated                :: Gene; CDS; rRNA; tRNA; ncRNA
            Genes (total)                     :: 2,776
            CDSs (total)                      :: 2,750
            Genes (coding)                    :: 2,714
            CDSs (with protein)               :: 2,714
            Genes (RNA)                       :: 26
            tRNAs                             :: 24
            ncRNAs                            :: 2
            Pseudo Genes (total)              :: 36
            CDSs (without protein)            :: 36
            Pseudo Genes (ambiguous residues) :: 0 of 36
            Pseudo Genes (frameshifted)       :: 2 of 36
            Pseudo Genes (incomplete)         :: 32 of 36
            Pseudo Genes (internal stop)      :: 2 of 36
            ##Genome-Annotation-Data-END##
FEATURES             Location/Qualifiers
     source          1..4968
                     /organism="Micropruina sp."
                     /mol_type="genomic DNA"
                     /submitter_seqid="NODE_7434_length_4968_cov_3.843069"
                     /isolate="AMR_MDS_2195"
                     /isolation_source="Activated sludge of municipal
                     wastewater treatment plants"
                     /db_xref="taxon:2737536"
                     /environmental_sample
                     /geo_loc_name="Canada"
                     /lat_lon="49.25 N 123.1 W"
                     /collection_date="2015-06-16"
                     /metagenome_source="wastewater metagenome"
                     /note="metagenomic"
     gene            471..1034
                     /locus_tag="PLF56_07410"
     CDS             471..1034
                     /locus_tag="PLF56_07410"
                     /inference="COORDINATES: protein motif:HMM:NF015648.2"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="PH domain-containing protein"
                     /protein_id="HOQ53425.1"
                     /translation="MTQNGLPPNDAGGFVTSPEKVYGLVDGVPTLGAPGADRLPSVAL
                     TYWRIHAAISGIVAAFMVSGVLAVAWPRVLVPWGLAMSILILLFTTFEIVAYLPFRYV
                     NYSYTVSEDFVYIAHGKILRRSVLIPATKIINVSSSQGPIMRSLRVTALGITHLLHVD
                     GFGPFPAELAENLKVRIVARSSAQRNP"
     gene            1085..2716
                     /locus_tag="PLF56_07415"
     CDS             1085..2716
                     /locus_tag="PLF56_07415"
                     /inference="COORDINATES: protein motif:HMM:NF015648.2"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="PH domain-containing protein"
                     /protein_id="HOQ53426.1"
                     /translation="MAYYLAAIPRFIAWIGLGLAWTQRTDQPLVPILAATSAVLIEIL
                     VPLYEWLFTWYRIAASGIEQRSGLIKRRTLRISWQAVRSVDSSQSWAERTTGTYTISL
                     KQESELEGHIKVVGATPAVKSAIENSLPTLLGHDVERSAEAYAGDPSLSAQGDSQAPE
                     SVRHPKMPPNSEVPIPTPCEPALGDDEPAVREGAPSGRVLFQARLSHVITGAFCSGTV
                     VLVAPSALLAVLDVVDDSPFGDALVGGLVAMPLPLAITVVVVIGVAAGLGVTVLRFHR
                     LRVIDTSEAVEVTYGWPGRTRRTIPRAALTGMIWTANPLEQVIGWRKLRFLTPESGAS
                     GTRPAALPALPNSVIAQILRTATHTVLRRASDSPALPEGSGSSLTGLSVALAGLGLRI
                     LSLICGVSTGWQLTRMGSPLWIGVAVGALLFWLMGALVRLLSSGFTYEGGLLVHRSSL
                     PGIRVTYQEISAIHGFSRWQLFSNTTPALLVTATSFTGRRLKLRSMRTDLSWLEPAER
                     DSVHASSSNGQAPPSTVSVATGRALETCPPPKEKQ"
     gene            2713..3597
                     /locus_tag="PLF56_07420"
     CDS             2713..3597
                     /locus_tag="PLF56_07420"
                     /inference="COORDINATES: protein motif:HMM:NF012235.2"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_function: GO:0005524 - ATP binding [Evidence IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="ATP-binding cassette domain-containing protein"
                     /protein_id="HOQ53427.1"
                     /translation="MIEVVSLTKRYGERLALDDVTFDVPAGSITAFVGPNGAGKTTLM
                     KIACGLLDATSGLALVDDEPFAKAADPLRTMGICLGPESLPGRMGGMRFLDYVARTQG
                     GTSRTPRDLFQAVGLVDERLPIGRYSTGMRQRLCVALAMLGEPANLILDEPMNGLDLV
                     GVRWLRETLRAEAQRGAAILLSSHILSELTVVADRVVMISAGTVVRQGRLQDVVARQG
                     RRGVYVEGPDNEALSRLIAAAGWHYERRGPGFLVQHDDPMVLARRCVETGVAFRHLSE
                     ASASLEDAFFETATMGVE"
     gene            3597..4385
                     /locus_tag="PLF56_07425"
     CDS             3597..4385
                     /locus_tag="PLF56_07425"
                     /inference="COORDINATES: ab initio
                     prediction:GeneMarkS-2+"
                     /note="Derived by automated computational analysis using
                     gene prediction method: GeneMarkS-2+."
                     /codon_start=1
                     /transl_table=11
                     /product="hypothetical protein"
                     /protein_id="HOQ53428.1"
                     /translation="MATQVREGRQTNTTVARAEAWRLSHSVRLLVAFGVCVAIGVLGV
                     VGTFELVGALSQGEIDVPRGSLAVSSGAFLVAASFGVVTIITMSREIADGTVSTMLRF
                     VPNRLRALIARSLALTAISLAAALVWAFASLAYVGLRGGLAADGASVPASLVGSVVAV
                     VLSVLVVNLIAVTVQNGVLSLLMTGLLFLVLPLIVSPIGVLGRLGLAQVGNVIYNVLP
                     CTFATNLMSPSLDRPEVVVGSLFGLLVWVGVAGYAAYLRFEKIT"
     gene            4764..>4968
                     /locus_tag="PLF56_07430"
     CDS             4764..>4968
                     /locus_tag="PLF56_07430"
                     /inference="COORDINATES: ab initio
                     prediction:GeneMarkS-2+"
                     /note="Derived by automated computational analysis using
                     gene prediction method: GeneMarkS-2+."
                     /codon_start=1
                     /transl_table=11
                     /product="hypothetical protein"
                     /protein_id="HOQ53429.1"
                     /translation="MLDLSQVTGTVTLGAIWAPSANLAFDSGLTTNGQWFGQNITTSG
                     DGEIHHYSFGGRLLCGSEPSPTPS"
BASE COUNT          833 a         1459 c         1606 g         1070 t
ORIGIN      
        1 ggtcgcgcta tctagtctta tttccgcgcg gcaatatcgc cgggtggtga cgagcgaggg
       61 gcgtgaatat tctctccttc gccatagtat ttgaacaatg atcgatattc ggatttgtaa
      121 tccaagatga gaatagtcat gggagctgta ctgcgattca tcgcctgggt attgcgccaa
      181 gcctggcgtt ggagcgtaaa acagatcgac aaggctgtgg cgtggatccg agccaactgg
      241 accacggtag caaagtggat tgctcagggc cttggcttcg agaccatcgt gtggcttctg
      301 tgccactact acggcctact ctgctgagcc caagcgtgac agcgggctgt ttgctctgcg
      361 ggtgttctca gtaaggggta acctggcgga gcccgagatg ccctagccga tagccgatga
      421 ctgcggcttc gactagggca actcgatgag tggctcgtaa ggttgcctta atgacacaga
      481 atggtttgcc accgaatgat gctggaggct tcgtgacttc tcccgagaag gtgtatgggc
      541 tggtggacgg tgttccgact cttggggcgc caggtgccga ccgcctcccc tccgttgcct
      601 tgacgtactg gcgcattcac gccgcgatat cgggaatcgt cgccgccttc atggtgtcgg
      661 gggtgctagc ggttgcgtgg ccccgcgtcc ttgtgccatg gggcttggcg atgtctatcc
      721 tcattcttct gttcactacc ttcgaaattg tggcttactt gccctttcgc tatgtcaatt
      781 actcctatac ggtaagtgag gatttcgtct atattgccca cggcaaaatt cttcgacgtt
      841 ccgttctgat acctgcaacg aaaataatca acgtgagttc ctcccagggt cccatcatga
      901 ggtcgttgcg agtaacggca cttggcatca cccatcttct gcacgtcgac ggctttggtc
      961 cgttccctgc tgagcttgct gaaaacctga aggtgcgcat tgtggcacgc tcctctgcgc
     1021 agcggaaccc atgacgcagc ccgtccacac aggcacgtgg tttcgggcca acgaacgtat
     1081 ggtcttggcc tactacctag ctgcgattcc tcgcttcatc gcatggatag gtctcggcct
     1141 tgcgtggacg cagcgcacgg atcagccgct cgtgcctatt ctggccgcta cctcagcggt
     1201 attgattgag attctggtac cactgtatga gtggctcttc acctggtacc gaattgcggc
     1261 atcaggcatt gagcagcgaa gcggtttgat caagcgccga actctgcgaa tatcctggca
     1321 ggcagtcagg tctgtggaca gctcgcagtc gtgggcagag cgaacaacag gtacctacac
     1381 aatctcgctc aagcaagaat ccgaactcga gggccacatt aaagtcgtcg gtgccactcc
     1441 cgcggtcaag agcgcgatcg aaaactctct cccgactctg ctcggccatg acgtggaaag
     1501 gtctgccgag gcatacgcgg gcgacccatc cttgtccgcg caaggcgatt cacaggcgcc
     1561 cgagagtgtc cgccacccca aaatgccacc gaactcggag gtgccgatcc ccactccttg
     1621 cgaacccgcg ctgggcgacg acgaacctgc tgtacgtgaa ggcgctccaa gcggaagggt
     1681 gctgtttcaa gcacgactga gtcacgtgat cacaggtgcg ttctgcagtg gcaccgtcgt
     1741 tctggtcgca ccttcagcgc ttctggctgt gttggacgtg gtcgacgact ccccgttcgg
     1801 cgacgccctt gtcgggggtc tggtggctat gccactcccg ttggcaatca cagtggttgt
     1861 cgtaatcggc gtggcggcgg gcctaggtgt cactgtgctg cggttccacc gattgcgggt
     1921 tatagatact tccgaggccg tggaggtgac ctacggatgg cctggccgca ccagacggac
     1981 aattccacgc gcggccctga ccggcatgat ctggacggcc aacccccttg aacaagtaat
     2041 cgggtggcgg aaactacgat tcctgacacc cgagagcggc gccagcggca cccgacctgc
     2101 cgcgctcccc gcgcttccca actcggtcat cgcacagatc ctccgaactg caacacacac
     2161 ggtgttgcga agagccagcg actcaccagc tctgcccgag gggtctggca gcagcctcac
     2221 gggcctgtcg gtcgcgttgg ccgggctcgg cctgcgcatc ctgagcctga tttgcggtgt
     2281 cagtactggt tggcagctca ctcgcatggg ctccccgctg tggatagggg tcgcggtcgg
     2341 tgcattactg ttctggctga tgggcgcact ggtgaggttg ctcagtagcg ggttcaccta
     2401 tgaaggcggt ctactcgtgc atcgctccag ccttcccgga atccgggtta cataccaaga
     2461 gatcagcgcg atccatggct tctctcgctg gcaactcttc agcaacacca cgccagcgct
     2521 tttggttacc gccacttcat tcaccgggcg aaggctgaag ttgcgatcga tgaggaccga
     2581 cctgtcatgg ctggagcctg cggagcgcga ttcggttcac gcatcgtcgt ccaacggtca
     2641 ggcaccccca tcaactgtgt ccgtcgccac tggccgagcc ctcgaaactt gtccgccccc
     2701 gaaggagaag cagtgattga agtggtgagc ttgaccaagc gctatggcga acgactcgcg
     2761 cttgatgacg taacgttcga tgttcccgca ggcagcatca ccgcgttcgt cggtcctaat
     2821 ggggccggca agaccacgct gatgaagatc gcgtgcggct tgcttgatgc gacctctggg
     2881 ctggctttgg tcgatgacga accgttcgcc aaagctgccg atcccctgcg aaccatgggc
     2941 atctgcctgg gtcccgagag cctgcccggc cgcatggggg gtatgcgatt cttggattac
     3001 gtagcgcgca cccagggtgg cacttcacga acaccgcgcg acctctttca ggccgtcggc
     3061 ctggttgatg agcgactgcc gatcgggcgc tactcgaccg gcatgcgcca acgcctgtgc
     3121 gtggcgctgg ccatgctggg cgagcccgcc aacttgattc tcgacgagcc gatgaacggc
     3181 ctagacctag tgggggtgcg ctggttgcgg gaaacgttgc gcgcggaggc gcagcgcggt
     3241 gcagctattc tgctgtcgtc gcatattctg tccgaactca cggtcgtcgc cgaccgagtg
     3301 gtgatgatct ctgcgggcac cgtcgttcgc caagggcggc tgcaggacgt ggtcgcgagg
     3361 caaggtcggc ggggggtgta cgtcgaaggt cccgacaacg aggcgttgtc gcggctgatc
     3421 gcggcggcag gctggcacta cgagcgaaga ggacccggtt ttctggtgca gcacgacgat
     3481 ccgatggttt tggctcgacg ctgtgttgag actggggtgg catttcgcca tctgtcggag
     3541 gcgtctgcgt cgctggaaga cgcattcttt gagaccgcga cgatgggagt cgagtgatgg
     3601 caacccaggt gcgcgagggg cggcagacga acaccaccgt ggcgcgagcc gaagcgtggc
     3661 ggttgagtca ctcggtacgg cttctcgtgg catttggggt gtgcgtcgcc atcggggttc
     3721 tcggcgtcgt gggaacattt gaactggtgg gcgctctgag tcaaggtgag atcgatgtgc
     3781 cccgtggctc tctagctgtg tcgagcggtg cctttctcgt ggccgcttcg ttcggcgtgg
     3841 tcaccatcat caccatgtcg cgcgagatcg ccgatggcac ggtctccacc atgctgcgct
     3901 tcgttccgaa ccggctgcgt gcgctgatcg cccgaagcct ggcactcacg gcgatcagcc
     3961 tggctgccgc cctggtgtgg gcattcgcct cgctcgcgta cgtcggtctg cgcggcgggc
     4021 ttgcggccga tggcgcgagc gtgccggcca gccttgtggg cagcgtcgtt gctgttgtgc
     4081 tgagcgtcct ggtcgtgaac ctgatcgccg taacggtgca gaacggcgtg ttgtcgctgc
     4141 tgatgaccgg gctgctgttt ctcgtgctgc cactgatcgt gtcgccgatc ggtgtcctcg
     4201 ggcggctggg cctggcgcag gtcggtaacg tcatttacaa cgtgttgccc tgcacctttg
     4261 cgaccaacct gatgagccca agtctcgatc ggcctgaggt agtcgtcggc agcctgttcg
     4321 ggttgttggt ctgggtcggc gtcgccggct acgccgccta cctgcgcttc gagaagatca
     4381 cctagtccga gaatgggtca cggcccgggt gcggttgagt gccaccacct gggtgggtgc
     4441 cggaaggcgg tttcgattat ccgaaggcgg gttcgagggt gacgcggtgg cggaggtgcc
     4501 tcgcgtgcgt gcacgtggtg cgctcggctc gggcagggtt gcggtgccgg tcgtagtgat
     4561 cggagccaag gtcggcgtat aggtctcggg attgctgagt aggtgccaga agatggccag
     4621 gacggatctg ccggtagtga ccgcggcttt cttcttgccg cgacgatgag cgatgagtcg
     4681 gtagcgggct ccgcctctcg gctttcgact tcgagggctg ggacccgggc acgacggcgg
     4741 gcaaggcgct gtcgcagtac ctgatgctcg atctgtcgca ggtcaccggc acggtcaccc
     4801 tcggtgcgat ctgggcgccg tccgccaatc tggcgttcga ctcggggttg acgaccaacg
     4861 ggcagtggtt cggccagaac atcaccacct cgggcgacgg tgagattcac cactacagct
     4921 tcggtggacg gctgctctgc ggctcggagc cgagtccgac gccgagca
//
LOCUS       DAOSUG010000193         4966 bp    DNA     linear   ENV 10-JUN-2024
DEFINITION  MAG TPA_asm: Micropruina sp. isolate AMR_MDS_2195
            NODE_7441_length_4966_cov_3.655467, whole genome shotgun sequence.
ACCESSION   DAOSUG010000193 DAOSUG010000000
VERSION     DAOSUG010000193.1
DBLINK      BioProject: PRJNA925477
            BioSample: SAMN32864262
            Sequence Read Archive: SRR4236673
KEYWORDS    WGS; ENV; Metagenome Assembled Genome; MAG; Third Party Data; TPA;
            TPA:assembly.
SOURCE      Micropruina sp. (wastewater metagenome)
  ORGANISM  Micropruina sp.
            Bacteria; Bacillati; Actinomycetota; Actinomycetes;
            Propionibacteriales; Nocardioidaceae; Micropruina.
REFERENCE   1  (bases 1 to 4966)
  AUTHORS   Abdulkadir,N., Saraiva,J.P., Zhang,J., Stolte,S., Gillor,O.,
            Harms,H. and Rocha,U.
  TITLE     Genome-centric analyses of 165 metagenomes show that mobile genetic
            elements are crucial for the transmission of antimicrobial
            resistance genes to pathogens in activated sludge and wastewater
  JOURNAL   Microbiol Spectr 12 (3), e0291823 (2024)
   PUBMED   38289113
REFERENCE   2  (bases 1 to 4966)
  AUTHORS   Abdulkadir,N.
  TITLE     Direct Submission
  JOURNAL   Submitted (24-MAR-2023) Environmental Microbiology, Helmholtz
            Center for Environmental Research -UFZ, Permoserstr 15, Leipzig
            04318, Germany
COMMENT     The annotation was added by the NCBI Prokaryotic Genome Annotation
            Pipeline (PGAP). Information about PGAP can be found here:
            https://www.ncbi.nlm.nih.gov/genome/annotation_prok/
            
            ##Genome-Assembly-Data-START##
            Assembly Method        :: SPAdes v. 3.11.1
            Genome Representation  :: Full
            Expected Final Version :: Yes
            Genome Coverage        :: Not Applicable
            Sequencing Technology  :: Illumina MiSeq
            ##Genome-Assembly-Data-END##
            
            ##Genome-Annotation-Data-START##
            Annotation Provider               :: NCBI
            Annotation Date                   :: 05/04/2023 04:57:10
            Annotation Pipeline               :: NCBI Prokaryotic Genome
                                                 Annotation Pipeline (PGAP)
            Annotation Method                 :: Best-placed reference protein
                                                 set; GeneMarkS-2+
            Annotation Software revision      :: 6.5
            Features Annotated                :: Gene; CDS; rRNA; tRNA; ncRNA
            Genes (total)                     :: 2,776
            CDSs (total)                      :: 2,750
            Genes (coding)                    :: 2,714
            CDSs (with protein)               :: 2,714
            Genes (RNA)                       :: 26
            tRNAs                             :: 24
            ncRNAs                            :: 2
            Pseudo Genes (total)              :: 36
            CDSs (without protein)            :: 36
            Pseudo Genes (ambiguous residues) :: 0 of 36
            Pseudo Genes (frameshifted)       :: 2 of 36
            Pseudo Genes (incomplete)         :: 32 of 36
            Pseudo Genes (internal stop)      :: 2 of 36
            ##Genome-Annotation-Data-END##
FEATURES             Location/Qualifiers
     source          1..4966
                     /organism="Micropruina sp."
                     /mol_type="genomic DNA"
                     /submitter_seqid="NODE_7441_length_4966_cov_3.655467"
                     /isolate="AMR_MDS_2195"
                     /isolation_source="Activated sludge of municipal
                     wastewater treatment plants"
                     /db_xref="taxon:2737536"
                     /environmental_sample
                     /geo_loc_name="Canada"
                     /lat_lon="49.25 N 123.1 W"
                     /collection_date="2015-06-16"
                     /metagenome_source="wastewater metagenome"
                     /note="metagenomic"
     gene            <1..484
                     /locus_tag="PLF56_07435"
     CDS             <1..484
                     /locus_tag="PLF56_07435"
                     /inference="COORDINATES: protein motif:HMM:NF015995.2"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_component: GO:0043190 - ATP-binding cassette (ABC)
                     transporter complex [Evidence IEA];
                     GO_function: GO:0022857 - transmembrane transporter
                     activity [Evidence IEA];
                     GO_process: GO:0055085 - transmembrane transport [Evidence
                     IEA]"
                     /codon_start=2
                     /transl_table=11
                     /product="glycine betaine ABC transporter
                     substrate-binding protein"
                     /protein_id="HOQ53430.1"
                     /translation="LAAEAAKGRSLADLAKLNRTIKLCANSEISSPDRSYGPDGLKEN
                     YGITVEVVPVEDGGGPLTVKALKDGKCDMADIYSADPAIARNGLVTLADPKGIVLPQN
                     VVPIVSPRVDEAAAAVIDKVSAALTTDELVAMNLLSVDKQQSSATIAKAWLTAKGLLS
                     "
     gene            complement(747..1244)
                     /locus_tag="PLF56_07440"
     CDS             complement(747..1244)
                     /locus_tag="PLF56_07440"
                     /inference="COORDINATES: protein motif:HMM:NF012755.2"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_function: GO:0003723 - RNA binding [Evidence IEA];
                     GO_function: GO:0004521 - endoribonuclease activity
                     [Evidence IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="ribonuclease domain-containing protein"
                     /protein_id="HOQ53431.1"
                     /translation="MSTARQRTLLAVGVVVVLLALVLGWGLGGGAGGPSAAGPETKPA
                     GASRTPSPTASRTPSRATAGTSGLPVCTSVPRQVNDTVAAVRANGPYLRPRDDGGTFG
                     NRERLLPAKPNGYYREYTVKTPGEGFPGPRRLITGGQASPGSEPTIWFYTADHYESFC
                     ELVLT"
     gene            complement(1241..1684)
                     /locus_tag="PLF56_07445"
     CDS             complement(1241..1684)
                     /locus_tag="PLF56_07445"
                     /inference="COORDINATES: protein motif:HMM:NF013500.2"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="barstar family protein"
                     /protein_id="HOQ53432.1"
                     /translation="MSTWTCLADELSLEGEALLFAADEREPALVQRSQAAGYRVVEVD
                     TAGVTDFRVAQARIAAVLRLPESAGRNLDALADSLGDLARYWPDDARIALFWRHPEKL
                     IERDTAGWFRLADVLQDATQRLWRGGNDPADRLFETVLLVPGFDA"
     gene            complement(1716..2210)
                     /locus_tag="PLF56_07450"
     CDS             complement(1716..2210)
                     /locus_tag="PLF56_07450"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_007077479.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_function: GO:0005524 - ATP binding [Evidence IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="universal stress protein"
                     /protein_id="HOQ53433.1"
                     /translation="MDAPLPIVVGNDGSDFARLALRKALWLAQQLKAEVRVVRAWSIS
                     TAPRPSTWSPGFVPPADDFEAIVKERLAKEVAPIAAEYPDVQVHLETPHGPAGRELLK
                     ASANARLLVVGTRGIGGFKGLLMGSVSNQVVEHAPCDVLVVRKGTDEVAPDRRVQLDR
                     FLDQ"
     gene            2269..3180
                     /locus_tag="PLF56_07455"
     CDS             2269..3180
                     /locus_tag="PLF56_07455"
                     /inference="COORDINATES: protein motif:HMM:NF012235.2"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_function: GO:0005524 - ATP binding [Evidence IEA];
                     GO_function: GO:0016887 - ATP hydrolysis activity
                     [Evidence IEA];
                     GO_function: GO:0140359 - ABC-type transporter activity
                     [Evidence IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="ATP-binding cassette domain-containing protein"
                     /protein_id="HOQ53434.1"
                     /translation="MTEHPIIEVRDFRMRFGDQVVIDDLSFDVHAGETFGLLGSNGCG
                     KTTTIRALLGIYEPTAGSLLVEGRRFDPAQGSRLGYLPEERGLYRKESVLEVMTYFGR
                     LKGLSRDAASGWSKEYLRRVDLADHAKVRLDKLSGGQQQKVQLGVTIMNAPSILILDE
                     PTKGFDPVNRRLLMDLVDEQRAQGSTVVMVTHQMDEVERLCDRILLLKDGRARAYGTV
                     AQVQDSFGGRRMRLTFSGVLPASPSYRVAVSERNYAELDLAEDVEPSAVVRELLDAGV
                     QLRGFETARVSLEEIFLKVYGEREAAA"
     gene            3177..4451
                     /locus_tag="PLF56_07460"
     CDS             3177..4451
                     /locus_tag="PLF56_07460"
                     /inference="COORDINATES: protein motif:HMM:NF024091.2"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="ABC transporter permease"
                     /protein_id="HOQ53435.1"
                     /translation="MSRHNLGTVISFEFRRTVLKPKFWLISLAVPLLIAVVVALITIS
                     GQTTADRAQSQDQEPVTFTYTDASGLIEPAVAKAAGGTEVADGAAARAAVVAGTSQCH
                     IDYPKDPLSQPIGVSGKDIGLFDSERYSAVATAVFQKSIVQRIGNPKLAQLAVNGPDV
                     QLVTFADGAATPGIAGVIVPALFLVLFYLAVLMLGNQMLNVTLEEKENRVTEMILTTM
                     NPTTLIVGKVIALVLIGIVQGIVVAIPASFAFSLLSNAIGAPATSAGTATLGSLPIDP
                     VRTIVGALLFLFSFLMFTGLLVAIGAVMPSAKEAGNTFGVVIISMFVPLYAASLLVSD
                     PHGVVSQVFTFFPLTAPVSALLRNATGGLYWWEASIALTVLALFGWLFLSLGVRLFRT
                     GSISYGSRVNIARALGWGRPSRRDNGGSRGTV"
     gene            4523..>4966
                     /locus_tag="PLF56_07465"
     CDS             4523..>4966
                     /locus_tag="PLF56_07465"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_007077710.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_function: GO:0008483 - transaminase activity [Evidence
                     IEA];
                     GO_function: GO:0030170 - pyridoxal phosphate binding
                     [Evidence IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="aminotransferase class III-fold pyridoxal
                     phosphate-dependent enzyme"
                     /protein_id="HOQ53436.1"
                     /translation="MTDRKGISPAQQAAIEQTETWGAHNYLPLPVVIESGQGAWLTDI
                     DGNRYLDFLSAYSAVNFGHANPVLTRAVAEQLGKVTITSRAVFSATYGPFVQALAQLC
                     GKQMVLPMNTGVEAVESGIKLARKWGYETKGVEERHANIIVMANNF"
BASE COUNT          780 a         1666 c         1731 g          789 t
ORIGIN      
        1 gttggcggcc gaggcggcca agggcaggag cctcgccgac ctggccaagc tgaaccgcac
       61 catcaagctg tgcgccaact ccgagatcag cagccccgac cgcagctacg gcccggacgg
      121 gctgaaagag aactacggca tcaccgtcga ggtcgtgccg gtcgaggacg gcggcggccc
      181 gctcaccgtc aaggcgctca aggacggcaa gtgcgacatg gccgacatct actccgccga
      241 cccggccatc gcccgcaacg gactggtgac gctggccgac cccaagggca tcgtcctgcc
      301 gcagaacgtg gtgccgatcg tctcgccccg ggtggacgaa gcggccgccg cggtgatcga
      361 caaggtgagt gccgcgctga ccaccgacga gctggtggcg atgaacctgc tcagcgtcga
      421 caagcagcag tcgtcggcga ccatcgcgaa ggcctggctg accgccaagg ggctgctcag
      481 ctgaaccctg cgattcgtcg ttgcgcctgg tcgcgtgcct cttggttgcg ttggtcgccg
      541 cggggctgga gcgatcgttc gcgactgtcg gctgcggccg ggccgacttc gtcggcccgg
      601 ccgcaggtgc ttcgtagtgt cgcttcacga tcgctccagc cccgcttcag cgttgcgttt
      661 cgagcgttga ggcccgggtg tgaacgtgcc gccggctcaa cgcaactgcc ttccaggcac
      721 gtttacccgg cctaagctga gtgggcctac gtgagcacca gctcgcagaa cgactcgtag
      781 tggtcggcgg tgtagaacca gatggtcggc tcggagcccg gactggcctg gccgccggtg
      841 atcagccggc gggggccggg gaacccctcg ccgggagtct tgacggtgta ttcgcggtag
      901 tagccgttcg gtttggcggg cagcaaccgc tcacggttgc cgaacgtgcc gccgtcgtcg
      961 cgcggacgaa gatagggccc gttcgcgcgc acggcggcga cggtgtcgtt cacctgccgc
     1021 ggcaccgagg tgcacaccgg aagaccggac gttcctgccg tggcgcgcga gggcgtccgg
     1081 gacgcggtgg gggagggcgt gcgggacgca ccggccggct tcgtttcggg accggccgcg
     1141 ctcggcccac ccgcgccgcc acccagcccc cagccgagca ccagggccag gagcaccacc
     1201 accaccccga ccgcgagcag ggtgcgctgc cgggccgtgc tcatgcgtcg aatcccggta
     1261 ccagcaggac ggtctcgaac agccggtccg ccgggtcgtt gccgccgcgc cagagccgct
     1321 gcgtcgcgtc ctgcagcacg tcggccagcc ggaaccagcc tgcggtgtcc cgctcgatca
     1381 gcttctcggg gtgccgccag aacagggcga tgcgtgcatc gtcgggccag tagcgggcga
     1441 ggtcgcccag gctgtcggcc aacgcgtcca ggttgcgtcc ggcgctctcg ggcagccgca
     1501 ggacggcggc gatccgggcc tgcgccaccc gaaaatcggt cacgcccgcg gtgtcgacct
     1561 cgaccacccg gtagccggcg gcctgcgagc gctgcaccag cgccggctcg cgctcgtcgg
     1621 ccgcgaagag caacgcctca ccctcgaggc tcagttcgtc ggccaggcac gtccaagtgc
     1681 tcatcgggga gacttcaccg gcgcacgaag gcgggtcact ggtcgaggaa acgatccagc
     1741 tgcacccggc gatcgggcgc cacctcatcg gtacccttgc gcaccaccag cacgtcacac
     1801 ggggcgtgct cgacgacctg attcgacacc gaccccatca gcagcccctt gaaacccccg
     1861 atgccgcggg tgccgaccac cagcagccgg gcgttcgcgg acgccttgag caactcgcgt
     1921 ccggccgggc cgtggggagt ctcgaggtgc acctgcacgt ccggatactc ggcggcgatc
     1981 ggtgcgacct ctttggccag ccgctccttg acgatggcct cgaagtcgtc ggccgggggc
     2041 acgaagcccg gcgaccaggt gctcgggcgg ggggccgtgc tgatcgacca ggcgcgcacg
     2101 acccgcacct cggccttgag ctgctgcgcg agccacagcg ccttgcgcag cgccagccgc
     2161 gcgaaatccg aaccgtcgtt gccgaccacg atgggcaagg gtgcgtccat ggggtgactc
     2221 taccgacgcc ggtggcggcg cgcttgttcg cttcgcaagg atgggggcat gaccgagcac
     2281 cccatcatcg aggtccgcga cttccggatg cgcttcggcg accaggtcgt gatcgacgat
     2341 ctgagcttcg acgtgcacgc cggtgaaacg ttcgggctgc tcggctcgaa cggctgcggc
     2401 aagaccacca ccattcgcgc cctgctcggc atctacgaac ccaccgccgg ctcgctgctg
     2461 gtcgagggca ggcgcttcga cccggcgcag ggctcgcggc tgggctacct gcccgaggag
     2521 cgcggcctgt accgcaagga gtcggtgctc gaggtgatga cctatttcgg ccggctcaag
     2581 gggctgagcc gcgacgcggc gtccggttgg tcgaaggaat acctgcgccg ggtcgatctg
     2641 gccgatcacg ccaaggtgcg cctcgacaag ctgtcgggcg gccagcagca gaaggtgcag
     2701 ctgggggtca cgatcatgaa cgcgcccagc attctcattc tggacgagcc gaccaagggc
     2761 ttcgacccgg tcaaccgccg gctgctgatg gatctggtcg acgaacagcg cgcgcagggc
     2821 tcgacggtgg tgatggtgac ccaccagatg gacgaggtcg agcggctgtg cgaccgcatt
     2881 ctgctgctga aggacggccg agccagggcc tacggcaccg tcgcgcaggt gcaggactcg
     2941 ttcggtggac ggcgcatgcg gctcacgttc agcggcgtgc tgcccgcctc gccgagctat
     3001 cgggtcgcgg tcagcgaacg caactacgcc gagctcgacc tggccgagga tgtcgagccg
     3061 agcgccgtcg tccgcgagtt gctcgacgca ggcgtgcagc tgcggggttt cgagaccgcg
     3121 cgggtctcgc tcgaagagat ctttctcaag gtgtacggcg aaagggaggc ggcggcatga
     3181 gccggcacaa cctcggcacg gtgatctcgt tcgagtttcg gcgcaccgtg ctcaagccca
     3241 agttctggct gatctcgctg gcggtgccgt tgttgatcgc cgtcgtggtg gcgttgatca
     3301 cgatcagcgg gcagaccacc gccgaccggg cccagagcca ggaccaggag cccgtcacct
     3361 tcacctacac cgacgcgtcg gggttgatcg agccggccgt cgcgaaggcg gccggcggca
     3421 ccgaggtggc ggacggggcg gcggcgcgcg cggcggtcgt cgcgggcacg tcgcaatgcc
     3481 acatcgacta ccccaaggac ccgctcagtc agccgatcgg ggtgagtggc aaagacatcg
     3541 gcctgttcga ctcggaacgc tactcggcgg tggccaccgc ggtgttccag aagtcgatcg
     3601 tgcagcggat cggcaacccg aagctggcgc agctcgccgt caacggtccg gacgtgcaac
     3661 tggtcacctt cgccgacggg gccgccacgc ccggcatcgc cggggtgatc gtgccggcgc
     3721 tgtttctggt gctgttctac ctggcggtgc tgatgctggg caatcagatg ctgaacgtca
     3781 cgctcgaaga gaaggagaac cgggtcaccg agatgatcct gaccacgatg aaccccacca
     3841 cgctgatcgt cggcaaggtg atcgcgctgg tgctgatcgg catcgtgcag ggcatcgtgg
     3901 tggcgatccc ggccagcttc gcgttcagcc tgctcagcaa cgcgatcggc gcgccagcga
     3961 cgtccgccgg caccgcgacg ctcggctcgc tgccgatcga cccggtgcgc acgatcgtcg
     4021 gggcgctgct gtttctgttc agcttcctga tgttcaccgg gctgctggtg gcgatcgggg
     4081 cggtgatgcc gtcggcgaag gaggcgggca ataccttcgg cgtggtgatc atctcgatgt
     4141 tcgtgcccct gtatgccgcc tcgttgctgg tcagcgaccc gcacggcgtg gtgtcgcagg
     4201 tgttcacctt ctttccgctc accgcgccgg tctcggcact gctgcgcaac gcgaccggtg
     4261 ggctgtactg gtgggaggcc tcgatcgccc tgaccgtgct ggccctgttc ggatggctgt
     4321 tcctgagcct gggggtacgg ctgttccgca ccggatcgat cagctacggc agccgggtca
     4381 acatcgcccg ggcactgggc tgggggcggc cgagtcgacg cgataatggt gggtcgcgcg
     4441 gcaccgtctg agctttttct tgtgtttgtt cgtctttgcc gcaagaatca gcgccttact
     4501 cgaatgaaac cggagaatcg ccatgaccga tcgcaagggc atcagcccgg cacaacaggc
     4561 cgccatcgag cagaccgaga cctggggagc gcacaactac ctgccgctgc cggtggtgat
     4621 cgaatcgggc cagggcgcct ggctgaccga catcgacggc aaccggtacc tcgactttct
     4681 ctcggcctac tcggcggtca acttcggcca cgcgaacccg gtgctgaccc gggccgttgc
     4741 cgagcagttg ggcaaggtga cgatcacctc acgcgcggtg ttctcggcga cctacggccc
     4801 tttcgtgcag gcgttggcgc agttgtgcgg caaacagatg gtgctgccga tgaacaccgg
     4861 cgtcgaggcg gtcgagtcgg gcatcaagct ggcgcgcaag tggggctacg aaaccaaggg
     4921 cgtcgaggag cgccacgcca acatcatcgt gatggccaac aacttt
//
LOCUS       DAOSUG010000194         4958 bp    DNA     linear   ENV 10-JUN-2024
DEFINITION  MAG TPA_asm: Micropruina sp. isolate AMR_MDS_2195
            NODE_7455_length_4958_cov_4.357128, whole genome shotgun sequence.
ACCESSION   DAOSUG010000194 DAOSUG010000000
VERSION     DAOSUG010000194.1
DBLINK      BioProject: PRJNA925477
            BioSample: SAMN32864262
            Sequence Read Archive: SRR4236673
KEYWORDS    WGS; ENV; Metagenome Assembled Genome; MAG; Third Party Data; TPA;
            TPA:assembly.
SOURCE      Micropruina sp. (wastewater metagenome)
  ORGANISM  Micropruina sp.
            Bacteria; Bacillati; Actinomycetota; Actinomycetes;
            Propionibacteriales; Nocardioidaceae; Micropruina.
REFERENCE   1  (bases 1 to 4958)
  AUTHORS   Abdulkadir,N., Saraiva,J.P., Zhang,J., Stolte,S., Gillor,O.,
            Harms,H. and Rocha,U.
  TITLE     Genome-centric analyses of 165 metagenomes show that mobile genetic
            elements are crucial for the transmission of antimicrobial
            resistance genes to pathogens in activated sludge and wastewater
  JOURNAL   Microbiol Spectr 12 (3), e0291823 (2024)
   PUBMED   38289113
REFERENCE   2  (bases 1 to 4958)
  AUTHORS   Abdulkadir,N.
  TITLE     Direct Submission
  JOURNAL   Submitted (24-MAR-2023) Environmental Microbiology, Helmholtz
            Center for Environmental Research -UFZ, Permoserstr 15, Leipzig
            04318, Germany
COMMENT     The annotation was added by the NCBI Prokaryotic Genome Annotation
            Pipeline (PGAP). Information about PGAP can be found here:
            https://www.ncbi.nlm.nih.gov/genome/annotation_prok/
            
            ##Genome-Assembly-Data-START##
            Assembly Method        :: SPAdes v. 3.11.1
            Genome Representation  :: Full
            Expected Final Version :: Yes
            Genome Coverage        :: Not Applicable
            Sequencing Technology  :: Illumina MiSeq
            ##Genome-Assembly-Data-END##
            
            ##Genome-Annotation-Data-START##
            Annotation Provider               :: NCBI
            Annotation Date                   :: 05/04/2023 04:57:10
            Annotation Pipeline               :: NCBI Prokaryotic Genome
                                                 Annotation Pipeline (PGAP)
            Annotation Method                 :: Best-placed reference protein
                                                 set; GeneMarkS-2+
            Annotation Software revision      :: 6.5
            Features Annotated                :: Gene; CDS; rRNA; tRNA; ncRNA
            Genes (total)                     :: 2,776
            CDSs (total)                      :: 2,750
            Genes (coding)                    :: 2,714
            CDSs (with protein)               :: 2,714
            Genes (RNA)                       :: 26
            tRNAs                             :: 24
            ncRNAs                            :: 2
            Pseudo Genes (total)              :: 36
            CDSs (without protein)            :: 36
            Pseudo Genes (ambiguous residues) :: 0 of 36
            Pseudo Genes (frameshifted)       :: 2 of 36
            Pseudo Genes (incomplete)         :: 32 of 36
            Pseudo Genes (internal stop)      :: 2 of 36
            ##Genome-Annotation-Data-END##
FEATURES             Location/Qualifiers
     source          1..4958
                     /organism="Micropruina sp."
                     /mol_type="genomic DNA"
                     /submitter_seqid="NODE_7455_length_4958_cov_4.357128"
                     /isolate="AMR_MDS_2195"
                     /isolation_source="Activated sludge of municipal
                     wastewater treatment plants"
                     /db_xref="taxon:2737536"
                     /environmental_sample
                     /geo_loc_name="Canada"
                     /lat_lon="49.25 N 123.1 W"
                     /collection_date="2015-06-16"
                     /metagenome_source="wastewater metagenome"
                     /note="metagenomic"
     gene            complement(199..2931)
                     /locus_tag="PLF56_07470"
     CDS             complement(199..2931)
                     /locus_tag="PLF56_07470"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_011756099.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_function: GO:0003676 - nucleic acid binding [Evidence
                     IEA];
                     GO_function: GO:0005524 - ATP binding [Evidence IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="DEAD/DEAH box helicase"
                     /protein_id="HOQ53437.1"
                     /translation="MSNAPDSILAAFAAGYGFSLDPFQVEACTHVEAGSGVLVAAPTG
                     SGKTVVGEFAVFLALQRGTKCFYTTPIKALSNQKFHDLVERHGAANVGLLTGDTSING
                     EAPVVVMTTEVLRNMIYANSRTLRDLGFVVMDEVHYLADRFRGAVWEEVIIGLAESVQ
                     VVSLSATVSNAEEFGAWLDEVRGDVRIVVSERRPIPLFQHVMAGRRLYDLFADEAPTA
                     RPQPTGHVDVNPELLRLAKEESRGVRDDSRKLRSRRVRGEIAKRNPRSSLIPRRDATV
                     ERLDAEALLPAIFFIFSRAGCDAAVGQLMHSGLRLTNAAERRELEQIAAAHTEGLSPS
                     DLDVLDYAGFVEAFRNGIAAHHAGLLPAFKEAVEEGFVRGLVKVVFATETLALGINMP
                     ARSVVLERLVKFNGETHAPITPGEYTQLTGRAGRRGIDVEGHAVVLWQSGMDPRSVAG
                     LASTRTYPLRSSFAPTYNMAVNLVGSVGRERARVLLEQSFAQYQSDRGVVGLARAIAR
                     NTEKIEGFLAQSACHRGDFAEYARLRAQISELEGEAARSRRADRRAEAMSVLLELKPG
                     DILKVPSGRHTGWAVVVDPGLAQGDPHPMVMTEERQLKRLSLVDFPTPPVVAGRMRVP
                     KHFDPRQPASRRNLSAAFRSKLAEIDLSAPRYSPTALDGELADRIAALRQQVKAHPCH
                     DCPDRENHARWAEQALRLERDNAGLQRQAERRTNTIAVKFDRICSVLESLGYLGDGGT
                     EVTDAGRMLARIYAELDLVTAECIRAGVFDGLSAPQLAAVLSSLVFEARGGDRRRPPR
                     MPDRASEAAQTAVRGIWRQVGLTERDHRLERGPEPDIGFAESIHAWASGRPLSEVLDE
                     AGLTAGDFVRWARQVVDFAGQVAQAAGPGDLRQTCRDVVERVRRGVVDAAPLED"
     gene            complement(3246..4142)
                     /locus_tag="PLF56_07475"
     CDS             complement(3246..4142)
                     /locus_tag="PLF56_07475"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_014846991.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_component: GO:0005575 - cellular_component [Evidence
                     IEA];
                     GO_function: GO:0001727 - lipid kinase activity [Evidence
                     IEA];
                     GO_process: GO:0008150 - biological_process [Evidence
                     IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="YegS/Rv2252/BmrU family lipid kinase"
                     /protein_id="HOQ53438.1"
                     /translation="MTPIAGPCVLVANPTAGRGRAGRQLPTIVQALRTALPRADLQVI
                     ESRSEAHAEQVCRGAVAQASTLLVMGGDGMAHLGLNACAGTDIPLGVLPAGTGNDLCR
                     GLGVPLRVRDAIGALARGASRRVDLAHITKPDGAERWVGCVVSTGFDSRVALRTAAMR
                     VPMGSLAYAWSALAELRTFAPLRYRLVIDGQRRDLDSMAVFVCNAEFFGGGMRAAPGA
                     DVTDGLLDVTVIHPVSRLVLLQLLPSLYSGKFVRHPAVERFRAAQVVIDGDGLIGSGD
                     GELLGDVPLAVRAVPGALTVLG"
     gene            complement(4139..>4958)
                     /gene="tatC"
                     /locus_tag="PLF56_07480"
     CDS             complement(4139..>4958)
                     /gene="tatC"
                     /locus_tag="PLF56_07480"
                     /inference="COORDINATES: protein motif:HMM:TIGR00945.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_component: GO:0005886 - plasma membrane [Evidence IEA];
                     GO_process: GO:0015628 - protein secretion by the type II
                     secretion system [Evidence IEA]"
                     /codon_start=2
                     /transl_table=11
                     /product="twin-arginine translocase subunit TatC"
                     /protein_id="HOQ53439.1"
                     /translation="ISFAWLKPPPTSPDGVMSLGDHLRELRYRELFSLIFIVLGMIVI
                     AIFYNPVYTFLAQPWLQAKEILSVTNPELSTQMVLSGVTTPLTLAMKICGVGGLILAA
                     PIWLYQIWAYIVPALLAKEKRWALMFVGTALPLFLAGVAVGYWILPQGISVLMSFTPQ
                     SVPITNLIEIDKFLELMLQLMLVFGLGFLMPVVVVGANLVGVLKASALAKARLYVIFG
                     TFVFGAAATPSTDPFSMLALAIPMSVLFLGAEVICHFNDKRKAKRLAAELVATS"
BASE COUNT          856 a         1739 c         1677 g          686 t
ORIGIN      
        1 ggcaccggtc gaagacgcgc tggacgtcgg cgcggcggtc ggcggcgcgg cggtctcgga
       61 cgtggacgtg ggggtgccgg tgccaatggt gcagccggcc agggtgaaca cggcaacgac
      121 caccgctgcg agcagtctca tgagtggctc ctttcggtag ccgcacgcta cctggcacga
      181 gcgaccggac gcacccgctc agtcctccag cggcgcggcg tccaccacac cccggcgcac
      241 ccgctcgacc acgtcccggc acgtctgccg caaatcgccc ggcccggcgg cctgcgccac
      301 ctggccggcg aagtcgacca cctgccgggc ccaccggacg aagtcgcccg ccgtgagccc
      361 ggcctcgtcc agcacctcgc tcagcggacg tccggacgcc caggcgtgga tcgactcggc
      421 gaagccgatg tcgggttcgg ggccgcgctc gaggcgatgg tcgcgttcgg tcagcccgac
      481 ctgccgccag atgccgcgga ccgcggtctg cgcggcttca ctggcccggt cgggcatgcg
      541 gggcggacgg cgccgatcgc cgccgcgggc ctcgaagacc agggacgaca gcacggccgc
      601 cagctgcggc gcgctcaacc cgtcgaacac ccccgcccgg atgcactcgg ccgtgaccag
      661 atcgagctcg gcgtagatgc gggccagcat gcgtccggca tcggtgacct cggtgccgcc
      721 gtcgccgagg taacccagcg attcgagcac cgagcagatg cggtcgaact tcaccgcgat
      781 ggtgttggtg cgccgctcgg cctggcgctg caggcccgcg ttgtcacgct cgagccgcag
      841 cgcctgctcg gcccagcggg cgtggttctc acggtcggga cagtcgtggc acggatgcgc
      901 cttcacctgc tgccgcaggg cggcgatgcg gtcggcgagt tcgccgtcga gcgcggtcgg
      961 cgagtagcgg ggcgccgaca ggtcgatctc ggccagtttc gagcggaagg ccgccgacag
     1021 gttgcgccgc gaggcgggtt ggcgcggatc gaagtgcttg ggcacgcgca tgcgtccggc
     1081 caccacgggc ggggtcggaa agtccaccag agagagccgt ttgagttgac gttcctcggt
     1141 catcaccatg gggtgcggat cgccctgcgc caaccccgga tcgaccacca ccgcccaacc
     1201 ggtgtgccgc ccggacggca ccttgagaat gtcgcccggt ttcagttcga gcagcacact
     1261 catggcttcg gccctgcggt cggcccgacg gctgcgagcg gcctcgccct ccaactcaga
     1321 gatctgagcg cgcagccgcg cgtactcggc gaagtcgccc cggtgacacg cggactgggc
     1381 cagaaagccc tcgatcttct cggtgttgcg ggcgatcgcg cgggccaggc ccaccacgcc
     1441 ccggtcggac tggtactgcg cgaacgactg ctcgagcagc acccgggccc gttcacgtcc
     1501 gacggagccg accagattga ccgccatgtt gtaggtcggc gcgaacgaac tgcgcagcgg
     1561 ataggtgcgg gtcgaggcga ggccggcgac ggagcgggga tccatgccgc tctgccagag
     1621 caccaccgca tggccttcga catcgatgcc gcggcgtccg gcccgtccgg tcaactgggt
     1681 gtactcgccg ggcgtgatgg gtgcatgggt ctcgccgttg aacttgacca gccgctcgag
     1741 caccacgctg cgggccggca tgttgatgcc cagcgccagg gtttcggtgg cgaacaccac
     1801 cttgaccagc ccccggacga agccctcctc gaccgcctcc ttgaacgccg gcagcaaccc
     1861 cgcgtgatgg gcggcgatgc cgttgcggaa cgcctccacg aagccggcgt agtcgagcac
     1921 gtccagatcg gacgggctga gcccctcggt gtgcgcggcg gcgatctgct cgagctcgcg
     1981 gcgttcggcg gcgttggtga gccgcagccc cgaatgcatg agttgaccga cggcggcgtc
     2041 gcagccggcc cggctgaaga tgaagaagat cgccggcagc agcgcttcag cgtccagtcg
     2101 ttcgacggtg gcgtcgcggc gcgggatcag cgacgagcgc gggttgcgct tggcgatctc
     2161 gccccgcacc cgacgtgagc gcagcttgcg cgaatcgtca cggacgccgc gcgactcctc
     2221 cttggccaac cgcaacagtt ccgggttgac gtcgacgtga ccggtgggct gcggacgggc
     2281 ggtcggcgcc tcgtcggcga acaggtcgta caggcggcgt ccggccatca cgtgttggaa
     2341 cagcgggatc ggacgccgct cggacaccac gatccgcacg tcgccgcgca cctcgtcgag
     2401 ccaggcgccg aactcttcgg cgttgctgac ggtggccgac agcgacacca cctggacgct
     2461 ctcggcgagg ccgatgatca cctcttccca cacggccccg cggaagcgat cggcgaggta
     2521 gtgcacctcg tccatcacca cgaagccgag gtcgcgcagg gtgcgcgagt tggcatagat
     2581 catgttgcgc aacacctcgg tggtcatcac caccaccggc gcctcgccgt tgatcgaggt
     2641 gtcgccggtg agcagaccca cgttggcggc gccgtgacgc tcgacgaggt catggaactt
     2701 ctggttgctc agcgccttga tcggcgtggt gtagaagcat ttggtgcccc gttgcagggc
     2761 gagaaagacg gcgaactcgc cgaccacggt cttgcccgaa cccgtgggag cggccaccag
     2821 cacacccgag cccgcctcca cgtgcgtgca ggcctcgacc tgaaaggggt cgaggctaaa
     2881 gccgtagcct gcggcgaagg ccgccaggat gctgtcgggc gcgttgctca tcgctgccca
     2941 acctatcgcg ggcgtccgcg cagcccggga cccggccgac cggcgttgaa cggagtgggg
     3001 gacgcttcgc aacccaatgc acccaacaaa cctgacgcga acggagttga gaacggtgcc
     3061 caacccgatt caccccgcag acgtgagtcg ctgaacggag tgggggacgg ttcctagccg
     3121 attcacccga tgacgggcgt cccgggcgtc aagagaagga cgaggcctaa cggggcctaa
     3181 cggagtgagc agccaactcc gcccagacct caggctcaac gcgccggaga gtggacgtac
     3241 tccactcagc cgagcaccgt gagtgccccc gggacggcgc gcaccgccag cggcacgtcg
     3301 ccgagcaact cgccgtcacc gctgccgatc agcccgtcac cgtcgatgac cacctgggcg
     3361 gcacgaaacc gctcgaccgc cgggtgccgg acgaactttc cggagtacag gctcggcagc
     3421 aactgcagca gcaccaggcg cgacaccggg tggatcacgg tcacgtcgag cagtccgtcg
     3481 gtcacgtccg cgccgggagc ggcccgcatg ccgccgccga agaactcggc gttgcatacg
     3541 aagaccgcca tcgaatcgag gtcacgtcgc tgcccgtcga tcaccagccg ataccgcaac
     3601 ggagcgaacg tgcgcagttc ggccagcgcc gaccaggcat aggccaggga gcccatcggc
     3661 acgcgcatcg ctgccgtgcg caacgccacc cgcgaatcga agccagtgga caccacgcag
     3721 ccgacccagc gctcggcacc gtccggtttc gtgatgtggg ccaggtcgac gcgccggctc
     3781 gcaccccggg cgagggcccc gatcgcgtcg cgcacccgca gcggaacacc cagcccgcgg
     3841 cacaggtcgt tgccggtgcc ggccggcagc actccgagcg gtatgtcggt gccggcgcag
     3901 gcgttgagtc cgaggtgcgc catgccgtcg ccgcccatca ccagcaaggt ggacgcctgc
     3961 gccacggccc cacggcagac ctgttcggca tgggcttcgc tacgcgactc gatgacctgc
     4021 aggtcggccc ggggcagcgc cgtccgcaac gcctgcacga tcgtcggtaa ctgccggccg
     4081 gcgcggccac gtccggcggt ggggttggcc accagcacgc aggggccggc gatcggcgtc
     4141 acgaagtggc cacgagttcg gcggccaacc gcttggcctt gcgcttgtcg ttgaaatggc
     4201 agatcacttc tgcaccgaga aacagcaccg acatcggaat cgccagcgcc aacatcgaga
     4261 acggatcggt ggacggggtt gctgccgcac cgaacacgaa cgtgccgaag atcacgtaca
     4321 accgggcctt ggcaagcgcc gacgccttca acacgccgac caggttggca cccaccacga
     4381 ccaccggcat caagaagccc aacccgaaca ccagcatcaa ctgcagcatc aactcgagaa
     4441 acttgtcgat ctcgatcagg ttggtgatcg gcaccgactg cggagtgaac gacatcagca
     4501 ccgagatgcc ctggggcagg atccaatacc cgaccgcgac ccccgccaga aacagcggca
     4561 gcgccgtgcc gacgaacatc aacgcccaac gcttctcttt cgccaacagc gccggcacga
     4621 tgtacgccca gatctggtac agccagatcg gcgcggccag gatcagcccg ccgaccccgc
     4681 agatcttcat cgccagggtc aatggggtgg tgacgcccga gagcaccatc tgggtgctca
     4741 actcggggtt ggtcacgctc aggatctctt tggcctgcaa ccacggctgc gccaggaagg
     4801 tgtagacagg gttgtagaag atcgcgatca cgatcatgcc cagcacgatg aagatcagcg
     4861 agaacagctc ccggtaccgc agttcgcgca ggtggtcgcc cagggacatc actccgtcgg
     4921 gcgacgtggg aggcggtttg agccaagcga aactgatc
//
LOCUS       DAOSUG010000195         4958 bp    DNA     linear   ENV 10-JUN-2024
DEFINITION  MAG TPA_asm: Micropruina sp. isolate AMR_MDS_2195
            NODE_7456_length_4958_cov_4.028962, whole genome shotgun sequence.
ACCESSION   DAOSUG010000195 DAOSUG010000000
VERSION     DAOSUG010000195.1
DBLINK      BioProject: PRJNA925477
            BioSample: SAMN32864262
            Sequence Read Archive: SRR4236673
KEYWORDS    WGS; ENV; Metagenome Assembled Genome; MAG; Third Party Data; TPA;
            TPA:assembly.
SOURCE      Micropruina sp. (wastewater metagenome)
  ORGANISM  Micropruina sp.
            Bacteria; Bacillati; Actinomycetota; Actinomycetes;
            Propionibacteriales; Nocardioidaceae; Micropruina.
REFERENCE   1  (bases 1 to 4958)
  AUTHORS   Abdulkadir,N., Saraiva,J.P., Zhang,J., Stolte,S., Gillor,O.,
            Harms,H. and Rocha,U.
  TITLE     Genome-centric analyses of 165 metagenomes show that mobile genetic
            elements are crucial for the transmission of antimicrobial
            resistance genes to pathogens in activated sludge and wastewater
  JOURNAL   Microbiol Spectr 12 (3), e0291823 (2024)
   PUBMED   38289113
REFERENCE   2  (bases 1 to 4958)
  AUTHORS   Abdulkadir,N.
  TITLE     Direct Submission
  JOURNAL   Submitted (24-MAR-2023) Environmental Microbiology, Helmholtz
            Center for Environmental Research -UFZ, Permoserstr 15, Leipzig
            04318, Germany
COMMENT     The annotation was added by the NCBI Prokaryotic Genome Annotation
            Pipeline (PGAP). Information about PGAP can be found here:
            https://www.ncbi.nlm.nih.gov/genome/annotation_prok/
            
            ##Genome-Assembly-Data-START##
            Assembly Method        :: SPAdes v. 3.11.1
            Genome Representation  :: Full
            Expected Final Version :: Yes
            Genome Coverage        :: Not Applicable
            Sequencing Technology  :: Illumina MiSeq
            ##Genome-Assembly-Data-END##
            
            ##Genome-Annotation-Data-START##
            Annotation Provider               :: NCBI
            Annotation Date                   :: 05/04/2023 04:57:10
            Annotation Pipeline               :: NCBI Prokaryotic Genome
                                                 Annotation Pipeline (PGAP)
            Annotation Method                 :: Best-placed reference protein
                                                 set; GeneMarkS-2+
            Annotation Software revision      :: 6.5
            Features Annotated                :: Gene; CDS; rRNA; tRNA; ncRNA
            Genes (total)                     :: 2,776
            CDSs (total)                      :: 2,750
            Genes (coding)                    :: 2,714
            CDSs (with protein)               :: 2,714
            Genes (RNA)                       :: 26
            tRNAs                             :: 24
            ncRNAs                            :: 2
            Pseudo Genes (total)              :: 36
            CDSs (without protein)            :: 36
            Pseudo Genes (ambiguous residues) :: 0 of 36
            Pseudo Genes (frameshifted)       :: 2 of 36
            Pseudo Genes (incomplete)         :: 32 of 36
            Pseudo Genes (internal stop)      :: 2 of 36
            ##Genome-Annotation-Data-END##
FEATURES             Location/Qualifiers
     source          1..4958
                     /organism="Micropruina sp."
                     /mol_type="genomic DNA"
                     /submitter_seqid="NODE_7456_length_4958_cov_4.028962"
                     /isolate="AMR_MDS_2195"
                     /isolation_source="Activated sludge of municipal
                     wastewater treatment plants"
                     /db_xref="taxon:2737536"
                     /environmental_sample
                     /geo_loc_name="Canada"
                     /lat_lon="49.25 N 123.1 W"
                     /collection_date="2015-06-16"
                     /metagenome_source="wastewater metagenome"
                     /note="metagenomic"
     gene            complement(426..2342)
                     /locus_tag="PLF56_07485"
     CDS             complement(426..2342)
                     /locus_tag="PLF56_07485"
                     /EC_number="3.4.24.-"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_015071286.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_function: GO:0004222 - metalloendopeptidase activity
                     [Evidence IEA];
                     GO_process: GO:0006508 - proteolysis [Evidence IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="M13-type metalloendopeptidase"
                     /protein_id="HOQ53440.1"
                     /translation="MSTFDLDPAARPQDDLFWHVNGRWLASTPIPDDRPSYGATQVLR
                     EQAERAVRNIVTELSGAEPGTEAAKVEALYASFMQTDEVERRGLSPLQPLLAEIDELD
                     STPDLLDYFGRSILRGTGSPIAIDVDSDPGDPTRYALFAFQSGLGLPDEEYYRDERFT
                     AIREAYQAHVAETLALAGLDPDQARLVLQLETEIAARHHDKVRTRDLRAMYNPTRPDA
                     FAEHTDWRRMLAAAEVPPIEQLINAQPSFVTELSELIDAEWLPSWRAWARWHLLSALS
                     PYLPEAFVQARFGFYGTTLNGIPANRERWKRGITLVERCLGEAVGKLYVERHFSPVAK
                     QRMDELVANLVAAYRHSIGELDWMTEATRTQALDKLSKFTAKIGYPDQWRDYTALVID
                     ADDLIGNVLRSTAFEYAHEWSKLGGPIRKHEWLMTPQTVNAYYHPLRNEIVFPAAILQ
                     PPFFDEHADEAVNYGAIGAVIGHEIGHGFDDQGSTCDGDGKLRDWWTDADRAAFEQRT
                     RALIEQYNELTPEQTPGHPVNGELTIGENIGDLGGLTIAYLAWRLAVGETDPEPIDGL
                     TGPQRLFVSWAKAWEGKTRTEALITQLTTDPHAPDEFRCNQVVRNLPEFYDAFGVGGD
                     DALWLPPEQRVKIW"
     gene            2642..3718
                     /locus_tag="PLF56_07490"
     CDS             2642..3718
                     /locus_tag="PLF56_07490"
                     /inference="COORDINATES: ab initio
                     prediction:GeneMarkS-2+"
                     /note="Derived by automated computational analysis using
                     gene prediction method: GeneMarkS-2+."
                     /codon_start=1
                     /transl_table=11
                     /product="hypothetical protein"
                     /protein_id="HOQ53441.1"
                     /translation="MKARQHSDTRRRTTRLVGITAVVALLAATLTGLLARGAAADPTL
                     PPLSPADLLNRVATAQVDGMSATFTQRSDLGLPALPAGVGGDDDLQSALSLLTGTHTI
                     RVWTAGPERSRVSLVDASAESSVIRNGNQVWTWSSEQQKAGHATIDAKKPTTRPTGAP
                     TTPAEAIDRLLNAIGPSTEVSTSGTGYVAGRAVYQLILAPKDAASLIGNVRLSVDATT
                     FVPLGLRVLDRGGAEAISLVASTVDYGVPAASVFEFTPPAGVEVTELAGKPSDHPTGT
                     AKTRPATKTVGTGWTTVVVASIDGKATDATAKALLDSLPAVSGSWGHGRLLGTNLLNV
                     VITDDGRVAIGSVTEDRLYAALAR"
     gene            3715..4746
                     /locus_tag="PLF56_07495"
     CDS             3715..4746
                     /locus_tag="PLF56_07495"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_020577709.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_function: GO:0005524 - ATP binding [Evidence IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="ATP-binding cassette domain-containing protein"
                     /protein_id="HOQ53442.1"
                     /translation="MSRSPDAGSQAEPPPAGPTLPEVTSDSDRQGSPQTGMAIASSGL
                     SKHFGRQLVVDDLALAVPSGSVYGFIGPNGSGKTTTIRMLLGLVRPDSGSCALLGAPM
                     PGAAGRVLPRVGALVEGPAFHPYLSGTANLRRLDAADPSSDPATAAARIAAALDRVGL
                     GAAAGKRYRAYSLGMRQRLGIAAALLHPRDLLVLDEPTNGLDPQGTREVRHLISELAA
                     DGTTIFVSSHLLAEVEQVCTHVGVMRSGRLLTQGPLDDLRALTAPRFRLLTPDSDAAL
                     GVLRALGAPDAVAEADEVTGTLAGLEPARVVAALVTGGVAVRSFTPVTATLEDAFVQL
                     TGEGFDVSG"
     gene            4757..>4958
                     /locus_tag="PLF56_07500"
     CDS             4757..>4958
                     /locus_tag="PLF56_07500"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_020388886.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="hypothetical protein"
                     /protein_id="HOQ53443.1"
                     /translation="MRRTRPTGRLFGSELRLMFGRRRNRVGLATLLGLVVLVGVALKI
                     SALRHPGSRGGDLITSVAGNGIF"
BASE COUNT          747 a         1734 c         1694 g          783 t
ORIGIN      
        1 tcacccgcgc ctacacccaa acaccccttg acgaaacata ggagagtcgc acgcccggat
       61 ctccacgggt tgccgggatc ccaaggcaag gccgggatgc ccagtgacca acggaacgtg
      121 cagtccgaac cgccgtcatc ccggcgcacg ccgggatctc cacgggttgc cgggatccca
      181 aggcaaggcc gggatgccaa gtgaccaacg gaacgtgcag tcctaacccc agtcatcccg
      241 gcgtactgac agccgggatc tcggcgtacg ccaaaacctc gggacatgcc cggcatgaca
      301 agtgaccaac ggaacgtgca gtcctaaccc tcgtcatccc ggcgcacgcc gggatctcca
      361 cgggttgccg ggatgccggg ccttcgccgc gatcacgggc tgtcgccggc atcacgagcc
      421 ttgtttcacc agatcttgac ccgctgctcg ggcggcaacc agagcgcatc gtcgccgccc
      481 accccgaacg cgtcgtagaa ctccggcaga ttccgcacca cctggttgca gcgaaactcg
      541 tccggcgcgt gcggatcggt ggtcaactgg gtgatcaggg cctcggtgcg ggtcttgccc
      601 tcccacgcct tggcccacga gacgaacaac cgctgcggtc ccgtcagtcc gtcgatcggc
      661 tcggggtcgg tctcacccac agccaggcgc caggccagat aagcgatggt cagtccgccg
      721 aggtcgccga tgttctcacc gatcgtcaac tcgccgttga ccggatggcc gggggtctgc
      781 tcgggcgtca actcgttgta ctgctcgatc agggcccggg tgcgctgctc gaacgcggcg
      841 cggtcggcgt cggtccacca gtcgcgcagc ttgccgtcgc cgtcgcaggt ggacccctga
      901 tcgtcgaagc cgtggccgat ctcgtggccg atcaccgccc cgatcgcacc gtagttgacc
      961 gcttcgtcgg cgtgctcgtc gaagaacggc ggctgcagaa tggcggccgg aaacacgatc
     1021 tcgttgcgca gcgggtggta gtaggcgttg actgtctgcg gcgtcatcag ccattcgtgc
     1081 tttcgaatcg gaccgcccag tttcgaccac tcgtgcgcgt actcgaaggc ggtggaccgc
     1141 agcacgttgc cgatcaggtc gtcggcgtcg atcaccaggg cggtgtagtc gcgccactgg
     1201 tcggggtagc cgatcttcgc ggtgaacttg ctcaacttgt cgagggcctg ggtgcgggtg
     1261 gcctcggtca tccagtcgag ttcgccgatg gagtgccggt aggcggcgac caggttggcc
     1321 acgagttcgt ccatgcgttg tttggcgacg ggtgagaagt gccgctcgac gtacagcttg
     1381 ccgaccgcct cgcccaggca ccgttcgacc agggtgatgc cccgcttcca gcgttcgcgg
     1441 ttggccggaa tgccgttcag cgtggtgccg tagaagccga acctggcctg cacgaacgcc
     1501 tcgggcaggt agggcgacaa ggccgacagc aggtgccagc gcgcccacgc ccgccacgac
     1561 ggcagccact cggcgtcgat cagctcggac aactcggtga cgaacgatgg ctgggcgttg
     1621 atcaactgct cgatcggtgg cacctcggcg gcggccagca tgcgccgcca gtcggtgtgc
     1681 tcggcgaacg cgtcgggacg ggtcgggttg tacatggcgc gcaggtcacg ggtgcgcacc
     1741 ttgtcgtggt gccgcgcggc gatctcggtt tcgagctgaa gcaccaggcg ggcctgatcg
     1801 gggtccagcc ccgccagggc gagggtttcg gccacgtgcg cctggtaggc ctcgcgaatg
     1861 gccgtgaacc tctcgtcgcg gtagtactcc tcatcgggca gcccgaggcc ggactggaag
     1921 gcgaacagtg cgtaccgggt gggatcgccc gggtcggagt cgacgtcgat cgcgatcggt
     1981 gagccggtgc cgcgcagaat gctgcggccg aagtagtcga gcagatcggg agtgctgtcg
     2041 agttcgtcga tctcggccag cagtggctgc agcggcgaca acccgcgccg ttcgacctca
     2101 tcggtctgca tgaaggacgc gtacagcgcc tcgaccttgg cggcctcggt gcccggctct
     2161 gcgcccgaca gctcggtgac gatgttccgc accgcacgct cggcctgctc gcgcagcacc
     2221 tgcgtggccc cgtaggacgg ccggtcgtcg ggaatcggcg tgctggccag ccaccggccg
     2281 ttgacgtgcc agaacaggtc gtcctggggt cgggcggcgg ggtcgaggtc gaaggtgctc
     2341 acggccgcga tcctaaatga cgttgtcgcc cacctggcgg accgccacct tctggtggtt
     2401 actgtggcgc cgatagcgag cctgccacct tttggtggct atggataccc cactggggta
     2461 ttgatagcga ccagaaagtg gcgccggcgg gcgatcgggg tcgatgtgac accaagccgc
     2521 cccgttcaca accagaacct ggtcaatgca atctggcgac tcgtcacgga cgggctcaag
     2581 ccggtcagca gccgccacgg tcacaccaca ccggttggca gcagggggca gaatgtagct
     2641 catgaaggca cggcagcatt ccgacacccg acggcgcacc actcgactcg tgggcatcac
     2701 ggcggtcgtc gcgctcttgg ctgccaccct gaccgggcta ctcgcccgtg gggccgcggc
     2761 cgacccgacg ctgccgccgc tcagccccgc cgacctgctc aaccgggtcg ccacggccca
     2821 ggtcgacggc atgagcgcca ccttcaccca gcgcagcgat ctgggcctgc cagcactacc
     2881 ggccggcgtc ggcggcgacg acgatctgca gtcggcgctc agcctgctga ccggtaccca
     2941 caccattcgg gtctggacgg ccggccccga gcgcagccga gtctcgttgg tcgacgcaag
     3001 cgccgaatcg tccgtgatcc gcaacggaaa tcaggtctgg acctggtcga gcgaacaaca
     3061 gaaggccggc cacgccacca tcgatgcgaa gaagccgacc acccgtccga ccggtgcgcc
     3121 caccactccc gccgaggcca tcgaccggct gctgaacgcg atcgggccga gcaccgaggt
     3181 ctccaccagc ggcaccggct acgtcgccgg gcgtgccgtc taccagctca tcctggcccc
     3241 gaaggatgcg gccagcctga tcggtaatgt gcggctctcg gtggacgcga ccacgttcgt
     3301 tccgctgggc ctgcgggtgc tcgatcgcgg cggtgccgag gcgatcagcc tggtggcgtc
     3361 caccgtcgat tacggcgttc cggctgcgag cgtcttcgag ttcacaccgc ccgcgggcgt
     3421 cgaggtcacc gaactggccg gcaagccgtc cgatcacccc acgggtaccg cgaagacccg
     3481 tccagccacg aagacggtcg gcaccggctg gaccaccgtc gtggtggcct cgatcgacgg
     3541 caaggccacc gacgccacgg ccaaggcgct gctcgattcg ttgcccgccg tgtccggctc
     3601 ctggggccat ggccggctgc tcggcacgaa cctgctcaac gtcgtgatca ccgacgacgg
     3661 acgcgtcgcg atcggctcgg tgaccgagga tcggctgtac gccgccctgg cccggtgagt
     3721 cgatcgcccg acgccgggtc gcaggccgag ccgccacccg ccggaccgac cctgcccgag
     3781 gtgacctccg actccgaccg gcaagggtcg ccgcagaccg gaatggcgat cgcgtcgtcc
     3841 gggctgagca agcacttcgg tcgccaactg gtggtggacg acctggccct ggcggtgccg
     3901 tccggctcgg tctacgggtt catcggtccc aacggctcgg gcaagaccac cacgatccgc
     3961 atgctgctcg gcctggtccg gccggactcg ggctcgtgcg ccctgctcgg cgcgccgatg
     4021 ccgggggcgg ccggcagagt gctcccacgg gtgggagctc tggtcgaggg cccggcgttc
     4081 cacccctacc tgtcgggcac ggccaacctg cgcagactgg acgccgccga cccgtcgagc
     4141 gatccggcca cggccgccgc gcggatcgcc gcggcgctcg accgcgtcgg cctgggtgct
     4201 gcggccggca agcggtatcg cgcctactcc ctgggtatgc ggcagcggtt gggtatcgcg
     4261 gctgcgctgc tgcacccccg cgatctgctc gtgctggacg agcccaccaa cgggctcgac
     4321 ccgcagggca cccgcgaggt gcggcacctg atcagtgaac tcgcggccga cggcaccacc
     4381 atcttcgtgt ccagtcacct gctcgccgag gtcgagcagg tctgcacgca tgtcggtgtg
     4441 atgcggtccg ggcggctgct cacccagggc ccgctggacg atctgcgcgc acttaccgcg
     4501 ccccgcttcc ggctgctcac cccggactcg gacgccgccc tcggcgtgct gagggcgttg
     4561 ggtgcgccgg acgccgtcgc cgaggccgac gaggtcaccg gaaccctggc cggtctggag
     4621 ccggcccgcg tggtcgccgc gctggtgacc ggaggcgtcg ccgtccgatc gttcacgccg
     4681 gtcaccgcga ccctcgagga cgcgttcgtg caactgaccg gggagggctt cgatgtcagc
     4741 ggctgagggg gtgcgagtgc ggcgcacacg gcccaccggg cggctgttcg gctccgagtt
     4801 gcgactgatg ttcggacgcc gtcgcaaccg ggtcgggctg gccacgctgc tcggtctggt
     4861 ggttctggtc ggcgtcgcgc tcaagatctc ggcgctgcgt catcccggct cacgcggcgg
     4921 cgacctgatc acgtccgtgg ccggcaacgg catctttc
//
LOCUS       DAOSUG010000196         4952 bp    DNA     linear   ENV 10-JUN-2024
DEFINITION  MAG TPA_asm: Micropruina sp. isolate AMR_MDS_2195
            NODE_7464_length_4952_cov_4.854401, whole genome shotgun sequence.
ACCESSION   DAOSUG010000196 DAOSUG010000000
VERSION     DAOSUG010000196.1
DBLINK      BioProject: PRJNA925477
            BioSample: SAMN32864262
            Sequence Read Archive: SRR4236673
KEYWORDS    WGS; ENV; Metagenome Assembled Genome; MAG; Third Party Data; TPA;
            TPA:assembly.
SOURCE      Micropruina sp. (wastewater metagenome)
  ORGANISM  Micropruina sp.
            Bacteria; Bacillati; Actinomycetota; Actinomycetes;
            Propionibacteriales; Nocardioidaceae; Micropruina.
REFERENCE   1  (bases 1 to 4952)
  AUTHORS   Abdulkadir,N., Saraiva,J.P., Zhang,J., Stolte,S., Gillor,O.,
            Harms,H. and Rocha,U.
  TITLE     Genome-centric analyses of 165 metagenomes show that mobile genetic
            elements are crucial for the transmission of antimicrobial
            resistance genes to pathogens in activated sludge and wastewater
  JOURNAL   Microbiol Spectr 12 (3), e0291823 (2024)
   PUBMED   38289113
REFERENCE   2  (bases 1 to 4952)
  AUTHORS   Abdulkadir,N.
  TITLE     Direct Submission
  JOURNAL   Submitted (24-MAR-2023) Environmental Microbiology, Helmholtz
            Center for Environmental Research -UFZ, Permoserstr 15, Leipzig
            04318, Germany
COMMENT     The annotation was added by the NCBI Prokaryotic Genome Annotation
            Pipeline (PGAP). Information about PGAP can be found here:
            https://www.ncbi.nlm.nih.gov/genome/annotation_prok/
            
            ##Genome-Assembly-Data-START##
            Assembly Method        :: SPAdes v. 3.11.1
            Genome Representation  :: Full
            Expected Final Version :: Yes
            Genome Coverage        :: Not Applicable
            Sequencing Technology  :: Illumina MiSeq
            ##Genome-Assembly-Data-END##
            
            ##Genome-Annotation-Data-START##
            Annotation Provider               :: NCBI
            Annotation Date                   :: 05/04/2023 04:57:10
            Annotation Pipeline               :: NCBI Prokaryotic Genome
                                                 Annotation Pipeline (PGAP)
            Annotation Method                 :: Best-placed reference protein
                                                 set; GeneMarkS-2+
            Annotation Software revision      :: 6.5
            Features Annotated                :: Gene; CDS; rRNA; tRNA; ncRNA
            Genes (total)                     :: 2,776
            CDSs (total)                      :: 2,750
            Genes (coding)                    :: 2,714
            CDSs (with protein)               :: 2,714
            Genes (RNA)                       :: 26
            tRNAs                             :: 24
            ncRNAs                            :: 2
            Pseudo Genes (total)              :: 36
            CDSs (without protein)            :: 36
            Pseudo Genes (ambiguous residues) :: 0 of 36
            Pseudo Genes (frameshifted)       :: 2 of 36
            Pseudo Genes (incomplete)         :: 32 of 36
            Pseudo Genes (internal stop)      :: 2 of 36
            ##Genome-Annotation-Data-END##
FEATURES             Location/Qualifiers
     source          1..4952
                     /organism="Micropruina sp."
                     /mol_type="genomic DNA"
                     /submitter_seqid="NODE_7464_length_4952_cov_4.854401"
                     /isolate="AMR_MDS_2195"
                     /isolation_source="Activated sludge of municipal
                     wastewater treatment plants"
                     /db_xref="taxon:2737536"
                     /environmental_sample
                     /geo_loc_name="Canada"
                     /lat_lon="49.25 N 123.1 W"
                     /collection_date="2015-06-16"
                     /metagenome_source="wastewater metagenome"
                     /note="metagenomic"
     gene            11..241
                     /locus_tag="PLF56_07505"
     CDS             11..241
                     /locus_tag="PLF56_07505"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_011757418.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="DUF3263 domain-containing protein"
                     /protein_id="HOQ53444.1"
                     /translation="MLEFEKQWWAFSGTKETEIRERFGLSAPRYYQLLNDLIDTPEAL
                     AHDPLLVKRLRRQRATRQRERSAKRLGFSVKV"
     gene            complement(193..699)
                     /locus_tag="PLF56_07510"
     CDS             complement(193..699)
                     /locus_tag="PLF56_07510"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_012918880.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="hypothetical protein"
                     /protein_id="HOQ53445.1"
                     /translation="MPTPHLSRAHTIRRVGNLLNLSSLLGFAIARLGRAGLAQGPEGL
                     TLAEGYRPRFPVAGAFTVGNVVITASSVARLSDHHPDVLRHESAHAWQYFWCGGLPFL
                     PLYALAAGWSWLRTGDPASRNLFERGAGLHRGGYREAPVTNAGLRRLRRAIQTLTEKP
                     NRLAERSR"
     gene            complement(993..3116)
                     /locus_tag="PLF56_07515"
     CDS             complement(993..3116)
                     /locus_tag="PLF56_07515"
                     /inference="COORDINATES: protein motif:HMM:NF021489.2"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="DUF2207 domain-containing protein"
                     /protein_id="HOQ53446.1"
                     /translation="MFATISRWIAYPLGWLLLMAVPLMIDAVRAGTVTATSAENRFTV
                     VAFVGEYDARRSGDRLAIDVTEHITADFPRRYVNHGIERRLRSSYRGESVELANIRVT
                     GTNGKPIAFTRRSESGSDDVVLRIGSANTYVFGRVDYVISYTIGNAMVQAGDRQEIYL
                     DVNGTGWLQRFDRVQATLSVAPDLAGHLLGEEACYQGAAGSTETCAITRSGDSFSTEA
                     LALGPRQNMTIAVGFRPGTVARALPAAVARPGLLGTIGMPVAAAALLALALGVRWFRL
                     RGLLLRGDVPIQYAAPAVQPILAADFLGRPERGAAAQLAQLVLDGHAQLSSNAEPIGT
                     APQPRARLGRRQRRAVRDEVTVRLIDREQIDPMVRDICAALFGRGGSVQLGEVPQAEV
                     ALASRLRQNLLADSDLRRPGRLGALLLGFGYAGLILFGWFVIATGLDSPAWLFLGCGT
                     LAVVLLVAAAHYYPTLGRLTARGRALRDRLAGLHRFVTMAEADRIAWMQNAVDSPRVS
                     ASAVGGTGRDGSSGGRDHVDGAVGKGRRGRGEGESQGVDADADAGRATGGTLIDLYEP
                     LLPYAILFGVEDTWRQALGELYRTAPPAVRPQIDVPALTLSVAAARTSPARYRHVSPY
                     RSFWDSRPAWGEGWASRAGGEIGRAFDSWATSRGDDDGGGWGSGGSSGSNRSSGSWSS
                     SSRSSGSSGGGRSGGGIGGGGGGGW"
     gene            3324..4949
                     /gene="groL"
                     /locus_tag="PLF56_07520"
     CDS             3324..4949
                     /gene="groL"
                     /locus_tag="PLF56_07520"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_012923547.1"
                     /note="60 kDa chaperone family; promotes refolding of
                     misfolded polypeptides especially under stressful
                     conditions; forms two stacked rings of heptamers to form a
                     barrel-shaped 14mer; ends can be capped by GroES;
                     misfolded proteins enter the barrel where they are
                     refolded when GroES binds; Derived by automated
                     computational analysis using gene prediction method:
                     Protein Homology.
                     GO_function: GO:0005515 - protein binding [Evidence IEA];
                     GO_function: GO:0016887 - ATP hydrolysis activity
                     [Evidence IEA];
                     GO_process: GO:0006457 - protein folding [Evidence IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="chaperonin GroEL"
                     /protein_id="HOQ53447.1"
                     /translation="MAKLIEFNVEARRGLERGMNVLADAVKVTLGPKGRNVVLEKKWG
                     APTITNDGVSIAKEIELEDPYEKIGAELVKEVAKKTDDVAGDGTTTATVLAQAMVREG
                     LRNVTAGANPMGLKKGIEKAVAAIVDSLAAQSTDVETKEQIAATASISAADPTVGDII
                     AEAMDKVGKEGVITVEESNTFGLELELTEGMRFDKGYISPYFVTDPERMEAVLDDPYV
                     LIANSKISSLKDLLPVLEKVMQSGKPLLVIAEDTDGEALAGLIVNKIRGTFKSVAVKA
                     PGFGDRRKAMLTDIAILTGGQVISEEVGLKLDAVTLELLGRARSVLVTKDETTIIEGA
                     GDSEQIAGRVSQIRKEIENSDSDYDREKLQERLAKLAGGVAVIKVGAATEVELKERKH
                     RIEDAVRNAKAAVEEGIVPGGGVALLQAAKSVDLSDLVGDEAVGAQIVLGACSAPLKQ
                     IAANAGLEGGVVAEKVGNLPAGQGLNAATGEYVDLLAAGIIDPAKVTRSALQNAASIA
                     ALFLTTEAVIADKPEKAPAMPAGGDGGMGGMDF"
BASE COUNT          832 a         1744 c         1677 g          699 t
ORIGIN      
        1 cgatgcaggc attctcgagt tcgagaagca atggtgggcc ttctccggaa ccaaagagac
       61 cgagattcgg gagcggttcg gcttgtcggc gccgcgttac taccagttgc tcaacgacct
      121 gatcgacacg ccggaggcgc tggctcacga cccgttgctg gtcaagcggc tgcgtcgcca
      181 gcgggccacc cgtcagcgtg agcgttcggc caagcggttg ggcttctcgg tcaaggtctg
      241 aatcgccctc cgcaaccggc gcagaccggc attcgtcacc ggcgcctcgc ggtagccgcc
      301 gcggtgtagc cccgcccccc gctcgaagag gtttctggac gccgggtcgc ccgtgcgtag
      361 ccacgaccag ccggccgcca gcgcgtacag gggcagaaag ggtagcccgc cgcaccagaa
      421 gtactgccac gcgtgcgcgc tctcgtggcg cagcacgtcg gggtggtggt cggacaatct
      481 cgccacgctg gaggcggtga tgacgacgtt gccaacggtg aaggcgcccg cgaccggaaa
      541 ccgcggccgg tagccctcgg cgagggtcaa accctcgggg ccctgcgcaa ggccggcccg
      601 tccgagccgc gcgatcgcga agcccagaag cgacgagagg ttcagcaggt tgcccacccg
      661 tcggatcgtg tgggcgcggc tcaggtgcgg cgtcggcatg ccagcagtgt gacgcgactg
      721 agcggacgcc cgctactcag ctcagggtgt cactgctgcc agcggtggac ggcccggggc
      781 ccttgtgtcc acactgctca cgacagtgtt caccgaccac caccagaggc cggcccgggc
      841 cctcgtgcga gccgtgctca cgacagtgct taacggccac gaccagtgtg cgccacggca
      901 cccgcgccgc ccttcgcgcg atcacgatgg tgcgcagcag ctgtcaccgg ggtccggccg
      961 ggaacccgcc cccgctctgc tcgcgtccgc gctcaccagc cgccgccacc cccaccaccg
     1021 atgccgccgc cggagcgacc accgcccgac gacccggacg agcgcgatga gctcgaccag
     1081 cttccgctcg atcggttcga gccactcgaa ccgccggacc cccacccgcc gccgtcgtcg
     1141 tcgccgcgcg acgtggccca cgaatcgaac gccctgccga tctcaccacc ggcgcgggag
     1201 gcccagccct cgccccaggc gggccgcgaa tcccagaaac tgcggtacgg cgacacgtgt
     1261 cggtaccggg ccggtgaggt acgcgccgca gccacgctca acgtcagcgc gggcacgtcg
     1321 atctgcggcc ggacggccgg cggtgccgtc cggtacaact cgcccagcgc ctgacgccag
     1381 gtgtcttcga cgccgaacag aatcgcgtag ggcagcaacg gctcatacag gtcgatcagg
     1441 gtgccaccgg ttgcgcgccc ggcgtccgcg tccgcgtcca ccccctgact ctcgccttca
     1501 cccctgcccc ggcgtccctt cccgacagcg ccgtcgacat ggtcccttcc accggacgac
     1561 ccgtcccggc ctgtgcctcc gacggcggac gcgctgaccc ggggagagtc caccgcgttc
     1621 tgcatccagg cgatccggtc ggcctcggcc atggtgacga agcggtgcag tcccgcgagt
     1681 cggtcgcgca gggcacgtcc acgggcggtc agccggccca gggtggggta gtagtgggcg
     1741 gcggccacca gcaacaccac cgcgagggtg ccgcagccga ggaagagcca ggctggagag
     1801 tccagaccgg tggcgatcac gaaccagccg aacaggatca accctgcgta tccgaatccc
     1861 agaagcagcg caccgagtcg gcccgggcgg cgcaggtcgc tgtcggccag caggttctga
     1921 cgtagacgcg acgccagcgc cacctcggcc tgcggcacct cgccgagctg gacggagccg
     1981 ccccggccga acagagcggc gcagatgtcg cggaccatgg ggtcgatctg ctcgcgatcg
     2041 atcagtcgca ccgtcacctc gtccctgacg gctcgtcgtt gccggcggcc gagtcgggcg
     2101 cgaggctggg gagcggtgcc gatcggttcg gcgttgctgg agagctgggc gtgaccgtcg
     2161 agcaccagct gcgccagttg ggcggctgcg ccccgctcgg gtcgtccgag aaagtccgcg
     2221 gccaggatcg gttgcaccgc aggagccgcg tactggatcg gcacgtcgcc gcgcagcaac
     2281 aggccacgca gcctgaacca gcgcacgccc agggccaggg cgagcagcgc cgccgcggct
     2341 accggcatgc cgatcgtacc cagcagcccc ggacgcgcca ccgcagccgg cagcgcgcgg
     2401 gcgacggtgc ccggccggaa gccgacggcg atcgtcatgt tctgcctcgg ccccagcgcg
     2461 agcgcctcgg tgctgaagga gtcgccgctg cgggtgatcg cgcaggtctc ggtcgagccg
     2521 gcggcccctt gatagcaggc ctcttcgccg agcaggtgcc cggccagatc gggggccacg
     2581 ctcagcgtcg cctgcacgcg gtcgaagcgc tgcaaccacc ccgtgccgtt gacgtcgagg
     2641 tagatctcct gccggtcgcc cgcctgcacc atcgcgttgc cgatggtgta gctgatcacg
     2701 tagtcgacgc gtccgaacac gtaggtgttg gcgctgccga tccgcagcac gacatcgtcc
     2761 gagcccgact cgctgcgccg ggtgaacgcg atcggcttgc cgttggtgcc ggtcacgcgg
     2821 atgttcgcca actcgacgct ttcgccgcgg tagctcgaac gcagccgccg ctcgatgccg
     2881 tggttcacgt agcggcgcgg aaaatcggcg gtgatgtgct cggtgacgtc gatcgccagc
     2941 cggtcgccgg accggcgcgc gtcgtactcg cccacgaagg ccaccacggt gaagcggttc
     3001 tctgcagagg tggcggtcac ggtgcccgcc cgtaccgcat cgatcatcag cggcacggcc
     3061 atcagcagca gccagccgag cggatacgcg atccagcgcg agatggtggc gaacacccgg
     3121 tcagattagg cggtcggtgg gtccgattcg tttgcactcg ccatggtcga gtgctaaaca
     3181 tggtgttagc actctcacca tgagagtgct acccggtcag ttcgatgagg cccccgggcc
     3241 gtccgtcgcg ggcatcgaga tgaccacccg acctgactac ccgtgggccc cgtcccaccg
     3301 atgcagaagg attgcctgta cccatggcaa aactgatcga attcaacgtt gaggcccgcc
     3361 gcggccttga gcggggcatg aatgtcctcg ctgacgcagt caaggtgacg ctcggcccca
     3421 agggccgcaa cgtcgtgctt gagaagaagt ggggtgcacc caccatcacc aacgacggcg
     3481 tctccatcgc caaagagatc gagctcgaag acccctacga gaagatcggc gccgagctgg
     3541 tcaaagaggt cgccaagaag accgacgacg ttgccggtga cggcaccacc accgccaccg
     3601 ttctggctca ggccatggtg cgcgagggcc tgcgcaacgt gaccgccggc gccaacccga
     3661 tgggcctgaa gaagggcatc gagaaggccg tcgccgccat cgtcgattcg ctggccgcgc
     3721 agtccaccga tgtcgagacc aaagagcaga tcgcggccac cgcgtccatc tcggccgctg
     3781 accccaccgt cggcgacatc atcgccgagg cgatggacaa ggtcggcaaa gagggcgtga
     3841 tcacggtcga ggagtcgaac accttcggtc tcgaactcga gctcaccgag ggcatgcgct
     3901 tcgacaaggg ctacatctcg ccgtacttcg tcaccgatcc cgagcgcatg gaggccgtcc
     3961 tggacgatcc ctacgtgctc atcgccaaca gcaagatcag ctcgttgaag gacctgctgc
     4021 cggtgctcga gaaggtcatg cagagcggca agccgctgct ggtgatcgcc gaagacaccg
     4081 acggcgaggc tctcgcgggc ctgatcgtca acaagatccg cggcaccttc aagtcggtcg
     4141 ccgtcaaggc tcccggcttc ggtgatcgcc gtaaggccat gctcaccgac atcgccattc
     4201 tgaccggcgg ccaggtgatc tccgaagagg tcgggctcaa gctcgacgcc gtgacccttg
     4261 agctgctggg ccgggcgcgc tccgtgctgg tgaccaagga cgagaccacg atcatcgagg
     4321 gcgccggtga ctccgagcag atcgcgggcc gggtctcgca gatccgcaaa gagatcgaga
     4381 actccgactc cgactacgac cgcgagaagc tgcaggagcg gctggccaag ctggccggcg
     4441 gcgttgcggt gatcaaggtc ggcgcggcca ccgaggtcga gctgaaggag cgcaagcacc
     4501 gcatcgagga cgccgttcgc aacgcgaagg cggccgtcga agagggcatc gtccccggcg
     4561 gtggtgtggc gctgttgcag gccgccaagt ccgtcgacct ctccgacctg gtcggtgacg
     4621 aggccgtcgg ggcccagatc gtgctcggcg cgtgctccgc tccgctgaag cagattgcgg
     4681 cgaacgccgg tctcgagggt ggcgtcgtgg ccgagaaggt gggcaacctg cccgccggcc
     4741 agggcctcaa cgcggccacc ggcgagtacg tcgatctgct ggccgccggc atcatcgatc
     4801 cggccaaggt gacccgttcg gcgctgcaga acgcggcctc gatcgcggcc ctgttcctga
     4861 ccaccgaagc ggtcatcgcc gacaagcccg agaaggctcc ggccatgccg gccggcggcg
     4921 acggcggcat gggcggcatg gacttctgag tc
//
LOCUS       DAOSUG010000197         4942 bp    DNA     linear   ENV 10-JUN-2024
DEFINITION  MAG TPA_asm: Micropruina sp. isolate AMR_MDS_2195
            NODE_7483_length_4942_cov_3.687743, whole genome shotgun sequence.
ACCESSION   DAOSUG010000197 DAOSUG010000000
VERSION     DAOSUG010000197.1
DBLINK      BioProject: PRJNA925477
            BioSample: SAMN32864262
            Sequence Read Archive: SRR4236673
KEYWORDS    WGS; ENV; Metagenome Assembled Genome; MAG; Third Party Data; TPA;
            TPA:assembly.
SOURCE      Micropruina sp. (wastewater metagenome)
  ORGANISM  Micropruina sp.
            Bacteria; Bacillati; Actinomycetota; Actinomycetes;
            Propionibacteriales; Nocardioidaceae; Micropruina.
REFERENCE   1  (bases 1 to 4942)
  AUTHORS   Abdulkadir,N., Saraiva,J.P., Zhang,J., Stolte,S., Gillor,O.,
            Harms,H. and Rocha,U.
  TITLE     Genome-centric analyses of 165 metagenomes show that mobile genetic
            elements are crucial for the transmission of antimicrobial
            resistance genes to pathogens in activated sludge and wastewater
  JOURNAL   Microbiol Spectr 12 (3), e0291823 (2024)
   PUBMED   38289113
REFERENCE   2  (bases 1 to 4942)
  AUTHORS   Abdulkadir,N.
  TITLE     Direct Submission
  JOURNAL   Submitted (24-MAR-2023) Environmental Microbiology, Helmholtz
            Center for Environmental Research -UFZ, Permoserstr 15, Leipzig
            04318, Germany
COMMENT     The annotation was added by the NCBI Prokaryotic Genome Annotation
            Pipeline (PGAP). Information about PGAP can be found here:
            https://www.ncbi.nlm.nih.gov/genome/annotation_prok/
            
            ##Genome-Assembly-Data-START##
            Assembly Method        :: SPAdes v. 3.11.1
            Genome Representation  :: Full
            Expected Final Version :: Yes
            Genome Coverage        :: Not Applicable
            Sequencing Technology  :: Illumina MiSeq
            ##Genome-Assembly-Data-END##
            
            ##Genome-Annotation-Data-START##
            Annotation Provider               :: NCBI
            Annotation Date                   :: 05/04/2023 04:57:10
            Annotation Pipeline               :: NCBI Prokaryotic Genome
                                                 Annotation Pipeline (PGAP)
            Annotation Method                 :: Best-placed reference protein
                                                 set; GeneMarkS-2+
            Annotation Software revision      :: 6.5
            Features Annotated                :: Gene; CDS; rRNA; tRNA; ncRNA
            Genes (total)                     :: 2,776
            CDSs (total)                      :: 2,750
            Genes (coding)                    :: 2,714
            CDSs (with protein)               :: 2,714
            Genes (RNA)                       :: 26
            tRNAs                             :: 24
            ncRNAs                            :: 2
            Pseudo Genes (total)              :: 36
            CDSs (without protein)            :: 36
            Pseudo Genes (ambiguous residues) :: 0 of 36
            Pseudo Genes (frameshifted)       :: 2 of 36
            Pseudo Genes (incomplete)         :: 32 of 36
            Pseudo Genes (internal stop)      :: 2 of 36
            ##Genome-Annotation-Data-END##
FEATURES             Location/Qualifiers
     source          1..4942
                     /organism="Micropruina sp."
                     /mol_type="genomic DNA"
                     /submitter_seqid="NODE_7483_length_4942_cov_3.687743"
                     /isolate="AMR_MDS_2195"
                     /isolation_source="Activated sludge of municipal
                     wastewater treatment plants"
                     /db_xref="taxon:2737536"
                     /environmental_sample
                     /geo_loc_name="Canada"
                     /lat_lon="49.25 N 123.1 W"
                     /collection_date="2015-06-16"
                     /metagenome_source="wastewater metagenome"
                     /note="metagenomic"
     gene            <1..1064
                     /gene="lepA"
                     /locus_tag="PLF56_07525"
     CDS             <1..1064
                     /gene="lepA"
                     /locus_tag="PLF56_07525"
                     /EC_number="3.6.5.n1"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_020574580.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_function: GO:0003924 - GTPase activity [Evidence IEA];
                     GO_function: GO:0005525 - GTP binding [Evidence IEA]"
                     /codon_start=3
                     /transl_table=11
                     /product="translation elongation factor 4"
                     /protein_id="HOQ53448.1"
                     /translation="GLGVGEVGYLITGVKDVRQSRVGDTVTLTTRAATENLGGYRQPN
                     PMVYAGLYPIDGADFPELREALDKLQLNDAALTYEPETSGALGFGFRIGFLGLLHMEI
                     VRERLEREFNLDLISTAPNVVYRVVMEDRSEHLVTNPSEYPDGKVAEVWEPVVKATIL
                     TPTDYIGTILELCQARRGTQTGMDYLSEDRVEIRYQLPLAEIVFDFFDALKSRTKGYA
                     SLDYDAAGEQMAKLVKVDILLHGEPVDAFSAIVHTDAAYAYGVAMAKKLKELIPRQQF
                     EVPIQAAIGSRVIARETVRAMRKDVLAKCYGGDISRKRKLLEKQKEGKKRMKMVGRVE
                     VPQEAFVAALSTGETSGKK"
     gene            complement(1140..1922)
                     /locus_tag="PLF56_07530"
     CDS             complement(1140..1922)
                     /locus_tag="PLF56_07530"
                     /inference="COORDINATES: protein motif:HMM:NF012400.2"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_function: GO:0097159 - organic cyclic compound binding
                     [Evidence IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="cytochrome b5-like heme/steroid binding
                     domain-containing protein"
                     /protein_id="HOQ53449.1"
                     /translation="MFDVFGLPLHPLVVHAVVVLLPLSALGLIACVLVNRWRRHFAGL
                     SVLGLVIGAGSAFVAAASGNALAAQVGTPTAHQAIGQVLPWVATATLALAAVWYFMQR
                     GRDGLTPLTRGIGALAAVAAAASVVVTVLVGHSGATAVWGGTSAAAAPSTAGATAPST
                     AGASSTSTGASTATSTAGASASAQSYTLEQVKQHNTQADCWAAIDDGVYNLTDWIGQH
                     PGGREHIIALCGTDATAAFDAQHEGQQRPESQLTRFYIGTLA"
     gene            complement(2093..2695)
                     /locus_tag="PLF56_07535"
     CDS             complement(2093..2695)
                     /locus_tag="PLF56_07535"
                     /EC_number="3.4.21.105"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_014845645.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_component: GO:0016020 - membrane [Evidence IEA];
                     GO_function: GO:0004252 - serine-type endopeptidase
                     activity [Evidence IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="rhomboid family intramembrane serine protease"
                     /protein_id="HOQ53450.1"
                     /translation="MSSHTLSRRDSLVPARTAGIVIGAWTVLLWLIELVDQVTRGALE
                     MLGIQSWNVASLPSVFVAPLIHDDWAHLAANTLPFAALGFLVLLGGWGRALWATVVIV
                     VVSGLFAWLLSPPHTVTIGASGLVFGWLAYLLVRAVFTRNTAQIVIAVAVLVVYGSLL
                     WGVLPTQPGVSWQAHLGGALGGALAARLLHGRDRASTLQG"
     gene            complement(2758..3186)
                     /locus_tag="PLF56_07540"
     CDS             complement(2758..3186)
                     /locus_tag="PLF56_07540"
                     /inference="COORDINATES: protein motif:HMM:NF012400.2"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_function: GO:0097159 - organic cyclic compound binding
                     [Evidence IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="cytochrome b5-like heme/steroid binding
                     domain-containing protein"
                     /protein_id="HOQ53451.1"
                     /translation="MNPHPALVGAALALLTLTACSGGPSATSAAPASSAATSATATAT
                     TSATATGTQSSAAGSSSSATTSYTLADVATHNTQSDCWAAIDGGVYNLTEWINRHPGG
                     RDKIIALCGTDATKAFHGQHDDQARPNTQLADFKVGELAG"
     gene            3289..4188
                     /locus_tag="PLF56_07545"
     CDS             3289..4188
                     /locus_tag="PLF56_07545"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_020579017.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_function: GO:0042626 - ATPase-coupled transmembrane
                     transporter activity [Evidence IEA];
                     GO_function: GO:0140359 - ABC-type transporter activity
                     [Evidence IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="sugar ABC transporter permease"
                     /protein_id="HOQ53452.1"
                     /translation="MPNHRGLRSPGHLRHGRLSTLLLAPSLFGLSAFLIFPILIVIWL
                     SLNKWDLLAPMRFVGLANYADVLTDPTFGNSMWRTLIFVVSVIPLQTALGLFMAVLLT
                     RGLPGSALFRVIYVIPWICAPLALGVVWKWILAPYDGALNNLLGIGVEWLSNPALAMP
                     AVAGVTIWSQVGYTTLFFMAGLAAIPDQVLEAAQLDGADGWQVFWQIKLPLLGPTMFF
                     VLVTGVISAFQAFDQIYALTPNGGPEGTTDVIATRIYLEAFQGGFNLGRASVMALVLF
                     VILVVVTVVQQRFFADRITYDLS"
     gene            4175..>4942
                     /locus_tag="PLF56_07550"
     CDS             4175..>4942
                     /locus_tag="PLF56_07550"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_020579018.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_process: GO:0008643 - carbohydrate transport [Evidence
                     IEA];
                     GO_process: GO:0055085 - transmembrane transport [Evidence
                     IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="carbohydrate ABC transporter permease"
                     /protein_id="HOQ53453.1"
                     /translation="MTSADRAGRTAGATPRTTPSGEAQNQAEPVPVLRSSARTHRRRT
                     PLADALTYLVLLVAAVLTLAPFALSFMTAFKSPQQFASEPALNLPNPITTENFVRLFS
                     GDYSFISPIAVTAQMVAVILVGQLLFSIMAAYAFAKLRFAGRDILFWIYLATLMVPQV
                     VIVIPLYVMMVQTGMRNTFWSLVLPFMFGSPYAVFLLREHFRSIPNDLIDAARIDGAG
                     DWRILWQIVVPLSRGIVSTLAIITVVSHWNNFMWPLII"
BASE COUNT          769 a         1632 c         1711 g          830 t
ORIGIN      
        1 aggggttggg cgtgggtgag gtgggctatc tgatcaccgg ggtgaaggac gtccggcagt
       61 cgcgggtggg cgacaccgtc accctgacca cgcgggcggc caccgagaat ctgggcggtt
      121 accggcagcc gaacccgatg gtctacgccg ggctgtaccc gatcgacggg gccgactttc
      181 ccgaactgcg cgaggcgctg gacaagctgc agctcaacga cgccgcgctg acctatgagc
      241 cggagacgtc cggggcgttg gggttcggct ttcggatcgg ctttctgggc ctgctgcaca
      301 tggagatcgt ccgtgagcgg ctcgagcgcg agttcaacct cgacctgatc tcgaccgcgc
      361 cgaacgtggt gtaccgggtg gtgatggagg atcgcagcga gcatctggtg accaacccgt
      421 cggagtaccc cgacggcaag gtcgccgagg tctgggagcc ggtggtgaag gccaccatcc
      481 tcacccccac cgactacatc ggcacgattc tggagctgtg ccaggcccgc cgcggcaccc
      541 agaccggcat ggactacctc agcgaggacc gggtcgagat ccgctaccaa ctgccgctgg
      601 ccgagatcgt gttcgacttc ttcgacgcgc tgaagtcacg cacgaagggg tacgcctccc
      661 tcgactacga cgcggccggt gaacagatgg cgaagctggt caaggtcgac attctgctgc
      721 acggcgagcc ggtggacgcc ttcagcgcca tcgtgcacac ggatgcggcc tacgcctacg
      781 gggtggcgat ggccaagaag ctcaaggaac tcattccgcg ccagcagttc gaggtgccga
      841 tccaggccgc gatcgggtcg cgggtgatcg cgcgcgagac cgtccgggcg atgcgcaagg
      901 acgtgctggc caagtgctac ggcggcgaca tcagccgaaa gcgcaagctg ctcgagaagc
      961 agaaagaggg caagaagcgc atgaagatgg tcggccgtgt cgaggtgccg caggaggcgt
     1021 tcgtggccgc gctgtcgacg ggtgagacgt ccggtaagaa gtagcgcgag cgcccccctg
     1081 actatcgtgc gcctgcgaag cttcgcgggc gcacgatagt taggtggggg ttcggcggct
     1141 caggcgagcg tgccgatgta gaaccgggtc agttgcgact ctggacgctg ctgcccctcg
     1201 tgctgggcgt cgaaggcggc ggtggcgtcg gtgccgcaga gcgcgatgat gtgctccctg
     1261 ccgccgggat gctggccgat ccagtcggtg aggttgtaga cgccgtcgtc gatcgccgcc
     1321 cagcagtccg cctgggtgtt gtgctgcttg acctgctcga gggtgtagct ctgagcggac
     1381 gcgctcgcgc cggccgtgct ggtggcggtg gacgcgccgg tggacgtgct gctggcgccg
     1441 gcggtggacg gagccgtggc gccggcggtg gacggagccg ctgcggccga ggtgccgccc
     1501 cagaccgccg tcgcgccact gtggccgacc agcacggtga ccaccacgga cgcggccgcg
     1561 gccaccgcgg ccagggcgcc gatgccacgg gtcagcgggg tcagtccatc gcggccacgc
     1621 tgcatgaagt accacactgc agccagggcc agcgtcgcgg tcgccaccca cggcagcacc
     1681 tgaccgatcg cctggtgcgc ggtgggggtg ccgacctggg cagccagcgc gttgccggac
     1741 gcggccgcga cgaacgccga gccggcaccg atcaccaggc ccagcacgct cagcccggcg
     1801 aagtgacggc gccaccggtt caccagcacg caggcgatca gcccgagggc cgacaggggc
     1861 agcagcacca cgaccgcgtg cacgaccagt gggtgaaggg gtagaccgaa gacatcgaac
     1921 atcgcgggct cctcggggtc atgggggcag caagtcgttg acgtgtcaac aatacctggc
     1981 gccggcgcgg taccagagcc gtgaacctgt gaatcacctg tctgtttgcg ctcgcctgcc
     2041 tgcgcggacg gagtgaggtg gcgacaggct caatcgtcaa ccggccggaa cgtcagccct
     2101 gcagcgttga cgcgcggtcg cgtccatgca acaaccgggc ggcgagcgcg ccacccaggg
     2161 cgccgcccag atgcgcctgc cacgacacgc ccggttgggt cggcagcacg ccccacagaa
     2221 ggctgccgta gaccacgagc acggcgaccg cgatcacgat ctgcgccgtg ttgcgggtga
     2281 agacggcccg caccaggagg taggccaacc acccgaagac cagcccgctg gcgccgatcg
     2341 tcaccgtgtg gggtgggctc agtagccagg cgaacaggcc gctgaccacg acgatcacca
     2401 ccgtggccca cagcgcgcgg ccccagccgc cgagcagcac cagaaagccc aacgctgcga
     2461 acggcagggt gttggccgcc agatgcgccc agtcgtcgtg aatcagcggt gccacgaaca
     2521 ccgacggcag cgaggccacg ttccaggact ggatcccgag catctccagg gccccgcggg
     2581 tgacctggtc gacgagctcg atcagccaca gcagcaccgt ccaggcaccg atcacgatgc
     2641 ccgcggtgcg ggcaggtacg agggagtcgc gccgtgacag cgtgtggctg ctcatggcgt
     2701 ccagtctgcc ggggcgggtc aagtgggcag gctcggagca ccgagcacga acccgcctca
     2761 accggccagc tcaccgacct tgaagtcggc cagctgcgtg ttcggtctgg cctggtcgtc
     2821 atgctggccg tggaatgcct tcgtggcgtc tgtgccgcac agcgcgatga tcttgtcgcg
     2881 gccgccggga tgccggttga tccactcggt caggttgtag accccgccgt cgatggccgc
     2941 ccagcagtcg ctctgggtgt tgtgcgtggc gacgtccgcc agcgtgtagc tcgtggtggc
     3001 tgaactggac gaaccggctg cggacgactg cgtgccggtg gcggttgcgc tggtggtggc
     3061 ggtggcggtt gcgctggtgg cggcgctgct ggccggcgct gccgaggtgg cggacggccc
     3121 gccgctgcag gcggtcagcg tgagcagggc cagggcggca ccaacgagtg cggggtgcgg
     3181 gttcatcgcc caagagtagt gcctgacgca tcaaacatcc ttgtccgagg ggcctgttga
     3241 aatctcaaga tctggcggac cggtcccgcg gcaactaggc tccctgccgt gccgaaccac
     3301 cgcgggctgc gttcgccggg ccacctgcgc cacggccggc tctccaccct gctgctagcc
     3361 cccagcctgt tcgggttgtc ggccttcttg atctttccga tcctgatcgt gatctggctg
     3421 tccctgaaca agtgggacct gctcgccccg atgcgcttcg tcggactggc caactacgcc
     3481 gacgtgctca ccgaccccac cttcggcaac tcgatgtggc gcacgttgat cttcgtggtg
     3541 tcggtgattc cgctgcagac tgcgctgggg ctgttcatgg ctgtgctgct gacccgcgga
     3601 ctgcccggct cggcgttgtt ccgggtgatc tacgtcattc cctggatctg tgcgccgctc
     3661 gcgctcgggg tggtctggaa gtggattctg gcgccgtacg acggagcgtt gaacaacctg
     3721 ctcggcatcg gcgtcgagtg gttgtcgaac cccgccctgg cgatgccggc ggtcgccggg
     3781 gtgaccatct ggtcgcaggt cggctacacc acgttgttct tcatggccgg cctggccgcg
     3841 attcccgacc aggtgctcga ggccgcgcaa ctggacgggg ccgacggctg gcaggtgttc
     3901 tggcagatca aactgccgct gctcggcccg acgatgttct tcgtgctggt caccggcgtg
     3961 atctcggcct ttcaggcgtt cgaccagatc tatgccctga cccccaacgg cgggcccgaa
     4021 ggcaccaccg acgtgatcgc cacccgcatc tacctggaag cctttcaggg cggtttcaac
     4081 ctgggccggg cctcggtgat ggcgctggtg ctgttcgtca ttctcgtggt cgtcaccgtc
     4141 gtgcaacagc ggttcttcgc cgacaggatc acctatgacc tcagctgatc gcgcaggccg
     4201 caccgccggc gctacaccgc gcaccacccc gtccggcgag gcgcagaatc aggcggagcc
     4261 cgtgccggtg ctccggtcgt ccgctcgcac ccatcgccgc cgcaccccgc tcgcggacgc
     4321 cctgacctat ctggtgctgc tggtggcggc ggtgctgacc ctggccccct tcgcgctcag
     4381 cttcatgacc gcgttcaagt cgccgcagca gttcgcctcc gaaccggcac tgaacctgcc
     4441 caacccgatc accaccgaaa acttcgtgcg gctgttcagc ggcgactaca gcttcatcag
     4501 tccgatcgcg gtgaccgcac agatggtggc ggtgattctg gtcgggcagt tgctgttctc
     4561 gatcatggcg gcctatgcct tcgccaagct gcgcttcgcc ggccgcgaca ttctgttctg
     4621 gatctacctc gccaccttga tggtgccgca ggtggtgatc gtcattccgc tgtacgtgat
     4681 gatggtgcag accggcatgc gcaacacgtt ctggtcgctg gtgctgccgt tcatgttcgg
     4741 ctcgccgtac gcggtgtttc tgctgcgcga gcactttcgc agcattccca acgacctgat
     4801 cgatgccgcg cgcatcgacg gggccggcga ctggcgcatt ctgtggcaga tcgtggtgcc
     4861 gctgagcagg ggcatcgtct cgacgctggc gatcatcacc gtggtcagtc actggaacaa
     4921 cttcatgtgg ccgttgatca tc
//
LOCUS       DAOSUG010000198         4927 bp    DNA     linear   ENV 10-JUN-2024
DEFINITION  MAG TPA_asm: Micropruina sp. isolate AMR_MDS_2195
            NODE_7507_length_4927_cov_4.399631, whole genome shotgun sequence.
ACCESSION   DAOSUG010000198 DAOSUG010000000
VERSION     DAOSUG010000198.1
DBLINK      BioProject: PRJNA925477
            BioSample: SAMN32864262
            Sequence Read Archive: SRR4236673
KEYWORDS    WGS; ENV; Metagenome Assembled Genome; MAG; Third Party Data; TPA;
            TPA:assembly.
SOURCE      Micropruina sp. (wastewater metagenome)
  ORGANISM  Micropruina sp.
            Bacteria; Bacillati; Actinomycetota; Actinomycetes;
            Propionibacteriales; Nocardioidaceae; Micropruina.
REFERENCE   1  (bases 1 to 4927)
  AUTHORS   Abdulkadir,N., Saraiva,J.P., Zhang,J., Stolte,S., Gillor,O.,
            Harms,H. and Rocha,U.
  TITLE     Genome-centric analyses of 165 metagenomes show that mobile genetic
            elements are crucial for the transmission of antimicrobial
            resistance genes to pathogens in activated sludge and wastewater
  JOURNAL   Microbiol Spectr 12 (3), e0291823 (2024)
   PUBMED   38289113
REFERENCE   2  (bases 1 to 4927)
  AUTHORS   Abdulkadir,N.
  TITLE     Direct Submission
  JOURNAL   Submitted (24-MAR-2023) Environmental Microbiology, Helmholtz
            Center for Environmental Research -UFZ, Permoserstr 15, Leipzig
            04318, Germany
COMMENT     The annotation was added by the NCBI Prokaryotic Genome Annotation
            Pipeline (PGAP). Information about PGAP can be found here:
            https://www.ncbi.nlm.nih.gov/genome/annotation_prok/
            
            ##Genome-Assembly-Data-START##
            Assembly Method        :: SPAdes v. 3.11.1
            Genome Representation  :: Full
            Expected Final Version :: Yes
            Genome Coverage        :: Not Applicable
            Sequencing Technology  :: Illumina MiSeq
            ##Genome-Assembly-Data-END##
            
            ##Genome-Annotation-Data-START##
            Annotation Provider               :: NCBI
            Annotation Date                   :: 05/04/2023 04:57:10
            Annotation Pipeline               :: NCBI Prokaryotic Genome
                                                 Annotation Pipeline (PGAP)
            Annotation Method                 :: Best-placed reference protein
                                                 set; GeneMarkS-2+
            Annotation Software revision      :: 6.5
            Features Annotated                :: Gene; CDS; rRNA; tRNA; ncRNA
            Genes (total)                     :: 2,776
            CDSs (total)                      :: 2,750
            Genes (coding)                    :: 2,714
            CDSs (with protein)               :: 2,714
            Genes (RNA)                       :: 26
            tRNAs                             :: 24
            ncRNAs                            :: 2
            Pseudo Genes (total)              :: 36
            CDSs (without protein)            :: 36
            Pseudo Genes (ambiguous residues) :: 0 of 36
            Pseudo Genes (frameshifted)       :: 2 of 36
            Pseudo Genes (incomplete)         :: 32 of 36
            Pseudo Genes (internal stop)      :: 2 of 36
            ##Genome-Annotation-Data-END##
FEATURES             Location/Qualifiers
     source          1..4927
                     /organism="Micropruina sp."
                     /mol_type="genomic DNA"
                     /submitter_seqid="NODE_7507_length_4927_cov_4.399631"
                     /isolate="AMR_MDS_2195"
                     /isolation_source="Activated sludge of municipal
                     wastewater treatment plants"
                     /db_xref="taxon:2737536"
                     /environmental_sample
                     /geo_loc_name="Canada"
                     /lat_lon="49.25 N 123.1 W"
                     /collection_date="2015-06-16"
                     /metagenome_source="wastewater metagenome"
                     /note="metagenomic"
     gene            complement(<1..128)
                     /locus_tag="PLF56_07555"
     CDS             complement(<1..128)
                     /locus_tag="PLF56_07555"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_014167025.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="Fpg/Nei family DNA glycosylase"
                     /protein_id="HOQ53454.1"
                     /translation="MPEGHVTHRLADGLTAMFGGHAVRVSSPQGRFADEAALLDGGV"
     gene            complement(159..614)
                     /locus_tag="PLF56_07560"
     CDS             complement(159..614)
                     /locus_tag="PLF56_07560"
                     /EC_number="5.3.1.6"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_020391463.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_function: GO:0016853 - isomerase activity [Evidence
                     IEA];
                     GO_process: GO:0005975 - carbohydrate metabolic process
                     [Evidence IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="ribose-5-phosphate isomerase"
                     /protein_id="HOQ53455.1"
                     /translation="MRVHIAADHAAYDLKEFLVKELTAAGYDVVDHGAHSYDSSDDYP
                     VFILPCAEAVAADPDSRGIVLGGSGNGEQIAANKVKGVRAGLAYNTVMARLAREHNDA
                     RIVALGGRMQSLSDALAMVKVFLETPFSNDPRHIRRIDMLTDYEERHAR"
     gene            complement(742..1341)
                     /locus_tag="PLF56_07565"
     CDS             complement(742..1341)
                     /locus_tag="PLF56_07565"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_014845196.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="DsbA family protein"
                     /protein_id="HOQ53456.1"
                     /translation="MTQHVDMWFDSVCPWAWMTSRWLLEVEKVRDVEVQFHVMSLSVL
                     NAGRDLPEQYQDLMAKALPPARVALAVQRDHPDKLTAYYTAIGTRLHPGGEELSDATV
                     AAALAEAGLPAELLDAGHTDANDDELERSHHEGMDPVGDEVGTPVLHINGMALFGPVI
                     SPAPKGEQAGDLFDGFVKLTAYPGFFEAKRTRTVGPIFD"
     gene            1618..4170
                     /gene="pepN"
                     /locus_tag="PLF56_07570"
     CDS             1618..4170
                     /gene="pepN"
                     /locus_tag="PLF56_07570"
                     /EC_number="3.4.11.2"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_002516958.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_function: GO:0004177 - aminopeptidase activity
                     [Evidence IEA];
                     GO_function: GO:0008237 - metallopeptidase activity
                     [Evidence IEA];
                     GO_function: GO:0008270 - zinc ion binding [Evidence IEA];
                     GO_process: GO:0006508 - proteolysis [Evidence IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="aminopeptidase N"
                     /protein_id="HOQ53457.1"
                     /translation="MYPANITRAEAAARARQLHITGYRVTVDLTGSATTFTSTSTIRF
                     TSSAGATFVDLIADSIVEATLDDAPLDTSGYRGERLDLDLAEGEHVLAITAVCRYSNT
                     GEGLHRFVDPADERVYCYTQLEVADARRVYACFEQPDLKASFTFAVIAPAHWTVLSNS
                     PEVTPIVAGDLGRWEFAATPPISTYITALIAGEYHTVRSVLQGRGAEIPLSLSCRISM
                     VPHLDADRLFETTSRGFEVFEQHFGAAYPFSDYAQVFVPEFNAGAMENAGCVTIRDEY
                     LYRSRVTAAAYEGRDNTILHELAHMWFGNLVTMTWWDDLWLNESFAEWASHFAQDEIR
                     KVHGGNDPWATFCNQRKTWAYRQDQLPSTHPIAADMVDLEAVELNFDGITYAKGASAL
                     RQLVAFVGLEPFLAGIRTYFSRHAWGNTQLGDLLEALEESSGRDLSFFAGQWLQTAGV
                     NTLSHQEEVDAKGRFTQFSVVQRAPEQWPTLRRHRIGVGLYDETETGIERVHRVEVDI
                     DGAVTEVEALAGVPAPAVVIVNDGDLSYAKIRLDRGLQVVVQRIQDFADPLTRALLWG
                     ATWDMCRDAQLSAADYVDLVVRGVPVETDLTAVGAVLAQAETAVRYYAPPAGRAALAA
                     RWTSGLAGLLKRVEPGSDHQLALVRALATSTTTPAGAELLSAWLAGDEVPEGLAVDAD
                     LRWRLVTNLARLGGIAEPGIAAELARDNTATGAEKAAGARAARPDAGAKAAAWQLAIE
                     DPSVPNETHTQLCAHFWQLDQNEVLTPYVDRWFAVMEDISAQRNGWGQRSLAIRKNVG
                     ELLFPRPLGDRALVTRINDWMAGTDLNDSVRRMVSERRDDVERALRCQEAAE"
     gene            4237..>4927
                     /locus_tag="PLF56_07575"
     CDS             4237..>4927
                     /locus_tag="PLF56_07575"
                     /inference="COORDINATES: protein motif:HMM:NF019604.2"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_function: GO:0016491 - oxidoreductase activity
                     [Evidence IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="FAD-dependent oxidoreductase"
                     /protein_id="HOQ53458.1"
                     /translation="MSGERVRTIVVVGGVAGGMSCAARARRLDESAEIIVLERGEHVS
                     FANCGLPYYVGGEIADAAALLVQTPQSLRSALNLDVRTGHEVTGVDPARKVVTVRTGS
                     GHHEIRYDALVLSPGAAAIRPAIPGIDSALVHTVRSVEDAVGLRAEVDARPAGRAVVL
                     GAGFIGLEVAEALAAQGMAVSVVELAAHVLPPLEREVAHDVEVELRGLGIAVHTGVPA
                     TAIEAVADHALI"
BASE COUNT          781 a         1715 c         1643 g          788 t
ORIGIN      
        1 acgccaccgt ccagcagcgc ggcctcgtcg gcgaagcgac cctgtgggct gctcacccgc
       61 acggcgtggc caccgaacat ggcggtcagc ccgtccgcga gccggtgggt gacgtgccct
      121 tcaggcatga tcgctcctgg gtcgaaccgg acgcccggtc agcgcgcgtg ccgttcttcg
      181 tagtcggtca gcatgtcgat gcgcctgatg tgccgggggt cgttcgagaa cggcgtctcg
      241 agaaacacct tcaccatcgc cagcgcatca ctgagcgact gcatgcgtcc gccgagggcg
      301 acgatgcggg cgtcgttgtg ctcgcgagcc agccgcgcca tcacggtgtt gtaggccagg
      361 ccggcccgca cgcctttgac tttgttggca gcgatctgct cgccgttgcc cgagccgccc
      421 agcacgatgc cccggctgtc ggggtcggcc gccaccgcct cggcgcacgg caggatgaag
      481 accggatagt catcggacga gtcgtagctg tgcgcgccat ggtccaccac gtcatagccg
      541 gcggcggtca gctccttgac caggaactcc ttcagatcgt aggccgcatg gtcggccgcg
      601 atgtgtacgc gcatggcgtc aacatagccg acccatgcgg gcgctcgtcc ccccgaacgc
      661 tcgcccaact atccagggct tgcgaagctt cgcgggtcct ggatacttgc gcgggcgttc
      721 ggcgtgaaga gcgacagctc ctcagtcgaa gatcgggccc accgtgcgcg tgcgcttggc
      781 ctcgaagaag cccgggtagg cggtcagttt gacgaacccg tcgaacaggt cgccggcctg
      841 ctcgcccttg ggcgccggcg agatcaccgg gccgaacagc gccatgccgt tgatgtgcag
      901 cacgggggtg cccacctcgt cgccgaccgg atccatgccc tcgtgatgcg agcgctcgag
      961 ctcgtcgtcg ttggcatcgg tatggccggc gtccagcagt tctgcgggca acccggcctc
     1021 cgcgagagca gcggccaccg tggcgtccga caactcctcg ccgccgggat gcagccgggt
     1081 gccgatcgcg gtgtagtacg cggtgagctt gtcggggtgg tcgcgctgca cagcaagtgc
     1141 aacccgggcc ggcggcaacg ccttggccat caggtcctgg tactgctcag gcaggtcacg
     1201 gcccgcattc agcaccgaca ggctcatcac gtggaactgc acctccacat cacgaacctt
     1261 ctcgacctcc agcagccagc gcgaggtcat ccatgcccag gggcagaccg agtcgaacca
     1321 catgtccacg tgttgcgtca tgcagcaacc ataggtggca tccaacccgg cgccggcacc
     1381 gttctgccgc agtgccggcc cgtcgcgatc aggcccgtcc ggtcgattcc ccgctactgc
     1441 acgcatcacc acggtccagg ctgatccggc cgcccgtggc ccaacccgga atgcgtgcag
     1501 ccactcacga ttacgcgtgc agcgaatgcc ccaaagggtg aagcggagac gatgacgatg
     1561 acagacggct ccgctactgt gccaagcgcg atccccaccg gtttctggag gaggaacatg
     1621 tatccggcca acatcacccg agccgaagcc gccgcacggg cacgacagct gcacatcacc
     1681 ggctatcggg taaccgtcga cctcaccggc tcggccacca ccttcacctc gacctcgacc
     1741 atccgcttca cgtcgtcggc cggcgccacc ttcgtcgacc tgatcgccga ctcgatcgtc
     1801 gaggccaccc tggacgacgc cccgctcgac acgtccggtt atcgggggga acgtctcgat
     1861 ctcgatctcg ccgagggcga gcacgtgctc gccatcaccg ctgtgtgccg ctacagcaac
     1921 acgggtgagg gtcttcaccg cttcgtcgac ccggccgacg agcgggtgta ctgctacacc
     1981 caactcgagg tcgcggacgc ccggcgcgtc tacgcctgct tcgaacagcc cgatctgaag
     2041 gcatccttca ccttcgccgt gatcgcgccc gcgcactgga cggtgctgtc caactccccc
     2101 gaggtcaccc cgatcgtggc cggtgacctg ggtcgctggg agttcgccgc cacaccgccg
     2161 atctccacct acatcaccgc cctgatcgcc ggcgagtacc acaccgtccg cagcgtgctg
     2221 cagggccgcg gcgccgagat tccgctctcg ctgtcctgcc ggatctcgat ggtgccccat
     2281 ctggacgccg accgactgtt cgaaaccacc tcccggggct tcgaggtttt cgagcagcac
     2341 ttcggggcgg cgtatccgtt cagcgactac gcgcaggtct tcgtgcccga gttcaacgcc
     2401 ggcgcgatgg agaacgccgg ctgcgtgacg atccgcgatg agtacctgta tcggtctcgt
     2461 gtgaccgcgg ctgcctatga gggccgcgac aatacgatcc tgcacgagct ggcacacatg
     2521 tggttcggca acctggtcac catgacttgg tgggacgacc tgtggctcaa cgaatcgttc
     2581 gcggaatggg cctcccactt cgcccaggat gagatccgca aggttcacgg cggcaacgat
     2641 ccctgggcca ccttctgcaa ccagcgcaag acctgggcat accggcagga ccagctgccc
     2701 tccacccatc cgatcgctgc cgacatggtc gacctggagg cggtcgagct caacttcgac
     2761 ggcatcacct acgccaaggg cgcctcggcc ctgcgtcaac tggtcgcgtt cgtcgggctg
     2821 gagccgttcc tggccggcat ccgcacctac ttctcccggc acgcctgggg caacacccag
     2881 ctcggcgacc tgctggaggc gttggaggaa tcgtccggac gggacctgtc cttcttcgcc
     2941 ggccagtggc tgcagaccgc cggggtgaac acgctgagcc accaggaaga ggtcgacgcc
     3001 aagggtcgat tcacccagtt cagcgtcgtt cagagagcac cggagcagtg gccgacgctg
     3061 cgccggcacc gtatcggcgt cgggctctac gacgagaccg agaccggcat cgagcgggtg
     3121 caccgggtcg aggtcgatat cgacggtgca gtgaccgagg tcgaggcgct ggccggcgtc
     3181 cctgccccgg cggtggtgat cgtcaacgac ggcgacctct cctatgccaa aatccggctc
     3241 gatcgcgggc tgcaggtggt ggtgcagcgc atccaggact tcgccgatcc gctcaccagg
     3301 gcgctgctgt ggggtgccac ctgggacatg tgccgcgacg cccagctgag tgcggccgac
     3361 tacgtggacc tggtggtgcg gggcgtcccg gtggaaaccg acctgacggc cgtcggcgcc
     3421 gtgctcgccc aggccgaaac cgctgtgcgc tactacgctc cgcctgccgg acgcgccgcg
     3481 ctggcggctc gctggacgtc cggactggcg ggcctgctga agcgcgtcga gccgggatcg
     3541 gaccaccaac tcgccctcgt ccgcgcgttg gcgacgtcga ccaccacgcc tgccggcgct
     3601 gaactgttgt cggcgtggct ggccggtgac gaagtgccgg aaggactggc ggtcgatgcc
     3661 gacctgcgct ggcggctggt caccaacctg gcccggctgg gcggcatcgc cgagcccggc
     3721 atcgccgccg aactggcccg ggacaacacc gccacgggtg ccgagaaggc ggccggggcc
     3781 agggccgcgc gcccggatgc cggggccaag gcggcggctt ggcagttggc catcgaggat
     3841 ccgtccgtgc cgaacgagac ccacacccaa ctgtgcgccc acttctggca gctcgatcag
     3901 aacgaggtgc tgacacccta cgtcgaccgg tggttcgcgg tgatggaaga catctctgcc
     3961 cagcgcaacg gatggggcca gcgctcgctg gcgatccgca agaacgtggg cgaactgctg
     4021 ttcccgcgtc cgttgggtga ccgtgccctg gtgacacgga tcaacgactg gatggccggg
     4081 acggacctca acgactccgt gcggcgcatg gtgagtgagc gtcgcgacga tgtcgaacgg
     4141 gcgctgcgct gccaggaggc cgccgaatag cctggcgacc ctcccactcc gcgcccagac
     4201 cagcgataca taccccctgg ggtatatggt gttggtgtga gtggagaacg agtaaggacg
     4261 atcgtggtgg tgggcggcgt cgccggtggg atgagttgcg cggcacgggc ccgccggctg
     4321 gatgaatcgg cggagatcat cgtgctggag cgaggcgagc acgtgtcctt cgccaactgc
     4381 ggactgccgt actacgtagg cggcgagatc gccgatgccg ccgcgctgct ggtgcagacc
     4441 ccgcagtcgc tgcgctcggc cctgaacctg gacgtgcgga ccggtcacga ggtgaccgga
     4501 gtcgacccgg cccgcaaggt cgtcacggta cgcaccggtt cgggccacca cgagatccgc
     4561 tacgacgcgc tggtgctctc ccccggggcg gcggcgatcc ggcccgccat ccccggcatc
     4621 gactccgccc tggtgcacac cgtccgcagc gtggaggatg ccgtgggcct gcgggccgag
     4681 gtggacgccc gtcctgccgg acgcgctgtg gtgctgggcg ccgggttcat cggcctggag
     4741 gtcgccgagg cgttggccgc acagggcatg gcggtctcgg tggtcgaact cgccgcacat
     4801 gtgctgccgc cgctggaacg cgaggtcgcg cacgacgtgg aagtcgagtt gcgagggctc
     4861 ggtatcgcgg ttcacaccgg ggttccggcc accgccatcg aagccgttgc cgatcatgcc
     4921 ctgatcc
//
LOCUS       DAOSUG010000199         4923 bp    DNA     linear   ENV 10-JUN-2024
DEFINITION  MAG TPA_asm: Micropruina sp. isolate AMR_MDS_2195
            NODE_7515_length_4923_cov_3.353328, whole genome shotgun sequence.
ACCESSION   DAOSUG010000199 DAOSUG010000000
VERSION     DAOSUG010000199.1
DBLINK      BioProject: PRJNA925477
            BioSample: SAMN32864262
            Sequence Read Archive: SRR4236673
KEYWORDS    WGS; ENV; Metagenome Assembled Genome; MAG; Third Party Data; TPA;
            TPA:assembly.
SOURCE      Micropruina sp. (wastewater metagenome)
  ORGANISM  Micropruina sp.
            Bacteria; Bacillati; Actinomycetota; Actinomycetes;
            Propionibacteriales; Nocardioidaceae; Micropruina.
REFERENCE   1  (bases 1 to 4923)
  AUTHORS   Abdulkadir,N., Saraiva,J.P., Zhang,J., Stolte,S., Gillor,O.,
            Harms,H. and Rocha,U.
  TITLE     Genome-centric analyses of 165 metagenomes show that mobile genetic
            elements are crucial for the transmission of antimicrobial
            resistance genes to pathogens in activated sludge and wastewater
  JOURNAL   Microbiol Spectr 12 (3), e0291823 (2024)
   PUBMED   38289113
REFERENCE   2  (bases 1 to 4923)
  AUTHORS   Abdulkadir,N.
  TITLE     Direct Submission
  JOURNAL   Submitted (24-MAR-2023) Environmental Microbiology, Helmholtz
            Center for Environmental Research -UFZ, Permoserstr 15, Leipzig
            04318, Germany
COMMENT     The annotation was added by the NCBI Prokaryotic Genome Annotation
            Pipeline (PGAP). Information about PGAP can be found here:
            https://www.ncbi.nlm.nih.gov/genome/annotation_prok/
            
            ##Genome-Assembly-Data-START##
            Assembly Method        :: SPAdes v. 3.11.1
            Genome Representation  :: Full
            Expected Final Version :: Yes
            Genome Coverage        :: Not Applicable
            Sequencing Technology  :: Illumina MiSeq
            ##Genome-Assembly-Data-END##
            
            ##Genome-Annotation-Data-START##
            Annotation Provider               :: NCBI
            Annotation Date                   :: 05/04/2023 04:57:10
            Annotation Pipeline               :: NCBI Prokaryotic Genome
                                                 Annotation Pipeline (PGAP)
            Annotation Method                 :: Best-placed reference protein
                                                 set; GeneMarkS-2+
            Annotation Software revision      :: 6.5
            Features Annotated                :: Gene; CDS; rRNA; tRNA; ncRNA
            Genes (total)                     :: 2,776
            CDSs (total)                      :: 2,750
            Genes (coding)                    :: 2,714
            CDSs (with protein)               :: 2,714
            Genes (RNA)                       :: 26
            tRNAs                             :: 24
            ncRNAs                            :: 2
            Pseudo Genes (total)              :: 36
            CDSs (without protein)            :: 36
            Pseudo Genes (ambiguous residues) :: 0 of 36
            Pseudo Genes (frameshifted)       :: 2 of 36
            Pseudo Genes (incomplete)         :: 32 of 36
            Pseudo Genes (internal stop)      :: 2 of 36
            ##Genome-Annotation-Data-END##
FEATURES             Location/Qualifiers
     source          1..4923
                     /organism="Micropruina sp."
                     /mol_type="genomic DNA"
                     /submitter_seqid="NODE_7515_length_4923_cov_3.353328"
                     /isolate="AMR_MDS_2195"
                     /isolation_source="Activated sludge of municipal
                     wastewater treatment plants"
                     /db_xref="taxon:2737536"
                     /environmental_sample
                     /geo_loc_name="Canada"
                     /lat_lon="49.25 N 123.1 W"
                     /collection_date="2015-06-16"
                     /metagenome_source="wastewater metagenome"
                     /note="metagenomic"
     gene            complement(<1..489)
                     /locus_tag="PLF56_07580"
     CDS             complement(<1..489)
                     /locus_tag="PLF56_07580"
                     /inference="COORDINATES: protein motif:HMM:NF016107.2"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="methyltransferase"
                     /protein_id="HOQ53459.1"
                     /translation="MQTAALVLYAAGLLAAFGLRTVWQLRRTGRSGWVGVSRHGGPAA
                     RVGAALFGAALLAGLFGVLAPAVGAVQVVSAGPGPAGVGLALTVAGLVVVLVSQAQMG
                     VSWRIGVDPGERTSLVTGGVFGWTRNPVFTGMVMVQVGVAFMVPSWWSMVAVLLLVAA
                     VQL"
     gene            654..1547
                     /locus_tag="PLF56_07585"
     CDS             654..1547
                     /locus_tag="PLF56_07585"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_020577771.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_function: GO:0008324 - monoatomic cation transmembrane
                     transporter activity [Evidence IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="cation diffusion facilitator family transporter"
                     /protein_id="HOQ53460.1"
                     /translation="MAVTTHRGRILLVLVLTSTVLVVEVIGAIITGSLALLADAGHML
                     TDVAGLVMALITATLMTRPASSTRTWGWRRAEILGATAQAAALFAVGVFVLVEGVRRL
                     FEPPDVASGLMVVFGVVGLVANAVGILILIGNRNANMNMRAAFLEVLNDTLGSVAVIV
                     AAVTIALTGWEQADALASILIALLILPRAFRLLREAVDILLEKTPADLDLDEVRRHIL
                     DLPHVQEVHDLHASQIATGLPVLSAHVIVDDECFTDGHAARLLTDLQDCVATHFPVSI
                     EHSTFQIEPAGHRTTEHTLHP"
     gene            1595..2257
                     /locus_tag="PLF56_07590"
     CDS             1595..2257
                     /locus_tag="PLF56_07590"
                     /inference="COORDINATES: protein motif:HMM:NF016149.2"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="copper resistance protein CopC"
                     /protein_id="HOQ53461.1"
                     /translation="MNRSALSPHLFRRSVTVLGLALLALVGFALPASAHTELTGSDPA
                     DGATVITAPASVTLSFSEGVQDFALSVVVTGPDGTQYAAGEPVADGTSVTTTVNPLGS
                     AGIYTVAYRIVSADDHPVTGQVTFAYAPPPVATTSLAGSAAPAAPASSSLSSSASPVT
                     AAADRSPTSSPVPRSTATAATAASSDTGTPFWMWVLVVVVTALLLVGGWFAWRSRSGN
                     AG"
     gene            complement(2297..3367)
                     /locus_tag="PLF56_07595"
     CDS             complement(2297..3367)
                     /locus_tag="PLF56_07595"
                     /EC_number="1.14.13.-"
                     /inference="COORDINATES: protein motif:HMM:NF019604.2"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_function: GO:0050660 - flavin adenine dinucleotide
                     binding [Evidence IEA];
                     GO_function: GO:0050661 - NADP binding [Evidence IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="NAD(P)/FAD-dependent oxidoreductase"
                     /protein_id="HOQ53462.1"
                     /translation="MSSDEPDVIVVGAGQAGMAVAYEVKRRGLQPILLDSGNRVGDTW
                     RRRWDSLVLFTPGRFDSLPGLPFPTGADDYPGKDAVADYLDRYARQMDLRIRLGTEVT
                     GVAGSFGSYMVSAGGRRHTAPQVVIATGPFHRPVIPSVAGGFDPAVTQLHTAEYRNPA
                     GLPPGPVLVVGAGNSGCQVARELSASRAVHLSTGDRNPVLPQRILGRDFFWWAGLVRF
                     DRITAQSRLGRRLQTRDPVFGDGPRRLARRCRVRLHPRLVGAEGRTARFTDGTTATVN
                     SVVWATGFTVDHSLVTVPGVLDEYGRIRQQGGVTSSRGLYTIGLPWQRTRGSALLGFV
                     GRDAADITDRISRDAAAARAAA"
     gene            complement(3402..4568)
                     /locus_tag="PLF56_07600"
     CDS             complement(3402..4568)
                     /locus_tag="PLF56_07600"
                     /inference="COORDINATES: protein motif:HMM:NF037017.2"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="Ig-like domain-containing protein"
                     /protein_id="HOQ53463.1"
                     /translation="MTQTVTAGAGPVTVSGLPGGSSTGSTSPVAVARFSASPRFGSTD
                     VAPADDVTVTAFNARIDRITAVSQTDGAELAGTLSEDGGTWTLTDRMDYATTYRITGT
                     ATGRDGAAVPVEGTIATVDPGETMRASFQLAEGGTYGVATPIILTFAGQVQDKAAAQQ
                     TFTVITDKGAVEGSWGWLQDEDIQGTGVKQSRVHFRTRDYWPANTTVTVEAKLYGIDL
                     GGGWGREDLTRTFTIGRSQIVQADVNSFRLVVTVDGAVAKNYPVSYGRNTDDPELETR
                     SGVHVVSEKFDSFTMCNPKYGYCGFIANWAVRISNNGEFIHENAAVIDSLGKANVSHG
                     CVNMGPGDAKDYYDSALYGDPVEVVGTSTQLAPSDGDIFDWTYDFDTWKTFSAL"
     gene            complement(4670..>4923)
                     /locus_tag="PLF56_07605"
     CDS             complement(4670..>4923)
                     /locus_tag="PLF56_07605"
                     /EC_number="3.4.23.36"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_011755657.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_component: GO:0016020 - membrane [Evidence IEA];
                     GO_function: GO:0004190 - aspartic-type endopeptidase
                     activity [Evidence IEA];
                     GO_process: GO:0006508 - proteolysis [Evidence IEA]"
                     /codon_start=3
                     /transl_table=11
                     /product="signal peptidase II"
                     /protein_id="HOQ53464.1"
                     /translation="WITVPGSPWFVRAGLAGVLAGASTNLIDRSIDGVVTDYLHTGWF
                     PTFNLPDVFITVGAALVVLGVLRPPQSTFPPVRTAPEDR"
BASE COUNT          752 a         1798 c         1652 g          721 t
ORIGIN      
        1 cagctgcacc gccgccacca gcagcagcac cgccaccatc gaccaccagg acggcaccat
       61 gaacgccacc cccacctgca ccatcaccat cccggtgaac accgggttgc gcgtccaccc
      121 gaacacgccg ccggtcacca gactcgttcg ctcccccgga tccaccccga tccgccacga
      181 cacccccatc tgcgcctgcg acaccagcac caccaccaga ccggccacgg tcagcgccag
      241 ccccaccccg gccggacccg ggcccgccga gaccacttgc accgcaccaa ccgccggagc
      301 gagcaccccg aacagacccg cgagcaacgc cgccccgaac agggccgcgc ccacccgcgc
      361 cgccggccct ccgtgccggg acacccccac ccagcccgat cgtcccgtcc gccgcagctg
      421 ccacaccgtc cgcagcccga acgcggccag cagaccagcc gcatacagca ccaacgccgc
      481 cgtctgcatc aaataccacc ctcctcagta catgattgcc tgaatacaga catcactgta
      541 ctgtctcggg aagtcggaac acatccccat cacccgaaag gcccaggtcg cggcatgcac
      601 agcactcatc acccggatgg cacgtcttga gcgggcacga ccatgaccac gccgtggcgg
      661 tcaccaccca ccggggccgg atcctgctcg tgcttgtcct gaccagcacg gtgctggtcg
      721 tcgaggtgat cggcgcgatc atcaccggca gcctggccct gctggccgac gccgggcaca
      781 tgttgaccga tgtcgccggc ctggtcatgg cgctgatcac cgcgaccctg atgacccgtc
      841 cggccagctc cacccgtacg tgggggtggc ggcgggcgga gatcctcggc gccacggcgc
      901 aggcggccgc cctgttcgcg gtcggggtgt tcgtgctggt cgagggcgtc cgccggctgt
      961 tcgagccgcc ggacgtcgcg tccggcctga tggtcgtgtt cggcgtggtc ggcctggtcg
     1021 ccaacgcggt cggcattctg atcctgatcg gcaaccgcaa cgcgaacatg aacatgcggg
     1081 cggcgttcct cgaggtgctc aacgacaccc tgggttcggt cgcggtgatc gtggccgcgg
     1141 tgaccatcgc gctcaccggt tgggagcagg ccgacgcgct cgcctccatc ctgatcgcgc
     1201 tgctcatcct gccgcgggcg ttccggctgt tgcgtgaggc ggtggacatc ctgctcgaga
     1261 agacgccggc cgacctggac ctggacgagg tccggcggca catcctggac ctgccgcacg
     1321 tgcaggaagt gcacgacctg cacgcctccc agatcgcgac cgggctgccc gtgctcagcg
     1381 cccacgtgat cgtcgacgac gagtgcttca ccgatgggca cgccgcccgc ctgctgaccg
     1441 acctacagga ttgcgtggcc acgcatttcc cggtcagcat cgaacattcg acgttccaga
     1501 tcgaaccggc cgggcaccgc acgaccgagc acaccttgca cccctgaccc cgaccggcga
     1561 ctgacccgtc acggacatgg agaaccgaac ccgaatgaac cgatccgcat tgtccccgca
     1621 cctgtttcgc cgctcggtga ccgttctcgg cctcgccctg ctggcgctgg tcgggttcgc
     1681 gctcccggcc agtgcgcaca ccgaactcac cggcagcgat cccgccgacg gtgccacagt
     1741 gatcactgcg ccggcctcgg tgacgctgtc cttcagcgag ggggtgcagg acttcgccct
     1801 gtccgtggtg gtaaccggcc ccgacggaac ccagtacgcg gccggagagc cggtcgcgga
     1861 cggcaccagc gtgacaacga cggtgaaccc gcttgggtcg gcgggaatct acacggtcgc
     1921 gtaccggatc gtctccgcgg acgaccaccc ggtcaccggc caggtcacct tcgcctacgc
     1981 ccctccgccg gtggccacca cctcactggc gggcagtgcc gcaccagccg ccccggcctc
     2041 gagcagcctg tcgtcgagcg cttcgccggt gacggccgcg gccgaccgca gtccgaccag
     2101 ctcgccggtc cccaggagta ccgcgaccgc ggcgacggcc gcctcgtctg acaccggaac
     2161 tcccttctgg atgtgggttc tcgtcgtggt cgtgaccgcg ctgctgctgg tcggcgggtg
     2221 gttcgcgtgg cgctcccgct cggggaacgc cggctgacag atccgcccgc cgagggccgc
     2281 tgcggagagc atggcgtcag gccgccgccc gggccgccgc ggcgtcccgg gaaatccggt
     2341 cggtgatgtc ggcggcgtcc cggccgacga atccgaggag tgcggaaccg cgggtgcgtt
     2401 gccagggcag gccgatcgtg tagaggccgc ggctggacgt gacgccaccc tgttggcgaa
     2461 tccggccata ttcgtcgagc accccgggaa cagtgacgag cgaatggtcg acggtgaatc
     2521 cggtggccca gaccaccgag ttcaccgtcg ccgttgtgcc gtcggtgaac cgggccgtcc
     2581 gcccttcggc gccgacgagt cggggatgca gccggacccg gcaacggcgg gcgagccgac
     2641 gcggcccgtc tccgaacacc ggatccctgg tttgcagtcg ccgacccagt cgggactgcg
     2701 cggtgatccg gtcgaaccgg accaggccgg cccaccagaa gaagtcccgg cccaggatcc
     2761 gttgcggcag gaccgggttg cggtcgccgg tggacaggtg gacggcgcgg gacgcggaca
     2821 gttcgcgggc aacctggcac cccgaattgc ccgcgccgac caccagcacc ggtccgggcg
     2881 gcaggccggc ggggttgcgg tattccgcgg tgtgcagttg ggtgactgcc gggtcgaagc
     2941 cgcctgcgac ggatgggatc accggccggt ggaacgggcc ggtcgcgatc accacctggg
     3001 gcgcggtgtg cctccggcca ccggcggaga ccatgtaact accgaaagat ccggccacgc
     3061 cggtgacctc ggtgcccaac cggatccgca ggtccatctg ccgggcgtac cggtcgaggt
     3121 agtcggcgac cgcgtccttg cccgggtagt cgtcggcgcc ggtcgggaac ggcagcccgg
     3181 gcaggctgtc gaatcggccg ggggtgaaca gcaccaggga gtcccagcgc cgccgccagg
     3241 tgtcgcccac acggttgccg gagtccagca ggatgggctg caggccgcgt cgtttcacct
     3301 cgtaggccac ggccatgccc gcctggccgg cgccgacgac gatcacatca ggttcgtcgg
     3361 aagacatcga tcctcctgct tgatcgtttc cggtaagcgg gtcacagggc cgagaacgtc
     3421 ttccaggtgt cgaagtcgta ggtccagtcg aagatgtcac cgtcggacgg cgccagttgg
     3481 gtgctggtcc cgacgacctc gaccgggtcc ccgtacaggg cgctgtcgta gtagtccttg
     3541 gcgtcccccg ggcccatgtt cacgcagccg tgcgagacgt tcgccttgcc cagagagtcg
     3601 atgacagcgg cgttctcgtg aatgaactcg ccgttgttgc tgatccggac ggcccagttg
     3661 gcgatgaatc cgcagtaacc gtacttgggg ttgcacatgg tgaagctgtc gaacttctcg
     3721 gagacgacgt ggaccccgct gcgggtctcc agttccggat cgtcggtgtt gcggccgtag
     3781 gaaaccgggt agttcttggc gaccgccccg tcgaccgtca ccaccagccg gaacgagttg
     3841 acgtccgcct gcacgatctg tgaccggccg atggtgaagg tgcgggtcag gtcctcccgg
     3901 ccccacccgc cgcccaggtc gatgccgtac agcttcgcct cgacggtcac cgtcgtgttc
     3961 gccggccagt agtcgcgggt gcggaagtgc acccgggact gcttgacgcc ggtcccctgg
     4021 atgtcctcgt cctgcaacca tccccacgac ccctcgaccg cacccttgtc ggtgatcacg
     4081 gtgaacgtct gctgggcggc cgccttgtcc tgcacctgcc cggcgaaggt caggatgatc
     4141 ggggtggcca cgccgtaggt gccgccctcg gcgagttgga acgacgcacg catcgtctcg
     4201 cccgggtcga cggtggcgat ggtcccttcg accgggaccg cggcaccgtc ccggccggtc
     4261 gccgtcccgg tgatccggta ggtcgtggcg tagtccatcc ggtcggtgag ggtccaggtg
     4321 ccgccgtcct cggacagcgt gccggccagc tccgcaccgt cggtctgcga tacggcggtg
     4381 atccggtcga tccgggcgtt gaacgcggtg acggtgacgt cgtccgcggg tgcgacgtcc
     4441 gtgctgccga accgcggtga cgcgctgaac cgggccaccg cgaccggtga ggtcgacccg
     4501 gtggacgatc cgccgggcag accgctgacg gtcaccggcc ccgcaccggc ggtcaccgtc
     4561 tgggtcacca ccaccgggtc cggctcgcct gccgagcacc ccccgatgag caggaccaag
     4621 acaccggcca gcagggcagc gcaccggcgg cggccgcgcg tcgcgccgat catcggtcct
     4681 ccggtgcggt gcggacgggc gggaacgtcg actgcggcgg ccggagcacc ccgagcacca
     4741 ccagggcggc gccgacggtg atgaacacgt cgggcaggtt gaaggtggga aaccagccgg
     4801 tgtgcagata gtcggtgacg accccgtcga tgctgcggtc gatcaggttg gtgctggcgc
     4861 cggccaggac tccggccagc ccggcccgga cgaaccaggg gctgcccggc acggtgatcc
     4921 agg
//
LOCUS       DAOSUG010000200         4920 bp    DNA     linear   ENV 10-JUN-2024
DEFINITION  MAG TPA_asm: Micropruina sp. isolate AMR_MDS_2195
            NODE_7523_length_4920_cov_3.893936, whole genome shotgun sequence.
ACCESSION   DAOSUG010000200 DAOSUG010000000
VERSION     DAOSUG010000200.1
DBLINK      BioProject: PRJNA925477
            BioSample: SAMN32864262
            Sequence Read Archive: SRR4236673
KEYWORDS    WGS; ENV; Metagenome Assembled Genome; MAG; Third Party Data; TPA;
            TPA:assembly.
SOURCE      Micropruina sp. (wastewater metagenome)
  ORGANISM  Micropruina sp.
            Bacteria; Bacillati; Actinomycetota; Actinomycetes;
            Propionibacteriales; Nocardioidaceae; Micropruina.
REFERENCE   1  (bases 1 to 4920)
  AUTHORS   Abdulkadir,N., Saraiva,J.P., Zhang,J., Stolte,S., Gillor,O.,
            Harms,H. and Rocha,U.
  TITLE     Genome-centric analyses of 165 metagenomes show that mobile genetic
            elements are crucial for the transmission of antimicrobial
            resistance genes to pathogens in activated sludge and wastewater
  JOURNAL   Microbiol Spectr 12 (3), e0291823 (2024)
   PUBMED   38289113
REFERENCE   2  (bases 1 to 4920)
  AUTHORS   Abdulkadir,N.
  TITLE     Direct Submission
  JOURNAL   Submitted (24-MAR-2023) Environmental Microbiology, Helmholtz
            Center for Environmental Research -UFZ, Permoserstr 15, Leipzig
            04318, Germany
COMMENT     The annotation was added by the NCBI Prokaryotic Genome Annotation
            Pipeline (PGAP). Information about PGAP can be found here:
            https://www.ncbi.nlm.nih.gov/genome/annotation_prok/
            
            ##Genome-Assembly-Data-START##
            Assembly Method        :: SPAdes v. 3.11.1
            Genome Representation  :: Full
            Expected Final Version :: Yes
            Genome Coverage        :: Not Applicable
            Sequencing Technology  :: Illumina MiSeq
            ##Genome-Assembly-Data-END##
            
            ##Genome-Annotation-Data-START##
            Annotation Provider               :: NCBI
            Annotation Date                   :: 05/04/2023 04:57:10
            Annotation Pipeline               :: NCBI Prokaryotic Genome
                                                 Annotation Pipeline (PGAP)
            Annotation Method                 :: Best-placed reference protein
                                                 set; GeneMarkS-2+
            Annotation Software revision      :: 6.5
            Features Annotated                :: Gene; CDS; rRNA; tRNA; ncRNA
            Genes (total)                     :: 2,776
            CDSs (total)                      :: 2,750
            Genes (coding)                    :: 2,714
            CDSs (with protein)               :: 2,714
            Genes (RNA)                       :: 26
            tRNAs                             :: 24
            ncRNAs                            :: 2
            Pseudo Genes (total)              :: 36
            CDSs (without protein)            :: 36
            Pseudo Genes (ambiguous residues) :: 0 of 36
            Pseudo Genes (frameshifted)       :: 2 of 36
            Pseudo Genes (incomplete)         :: 32 of 36
            Pseudo Genes (internal stop)      :: 2 of 36
            ##Genome-Annotation-Data-END##
FEATURES             Location/Qualifiers
     source          1..4920
                     /organism="Micropruina sp."
                     /mol_type="genomic DNA"
                     /submitter_seqid="NODE_7523_length_4920_cov_3.893936"
                     /isolate="AMR_MDS_2195"
                     /isolation_source="Activated sludge of municipal
                     wastewater treatment plants"
                     /db_xref="taxon:2737536"
                     /environmental_sample
                     /geo_loc_name="Canada"
                     /lat_lon="49.25 N 123.1 W"
                     /collection_date="2015-06-16"
                     /metagenome_source="wastewater metagenome"
                     /note="metagenomic"
     gene            210..1058
                     /locus_tag="PLF56_07610"
     CDS             210..1058
                     /locus_tag="PLF56_07610"
                     /inference="COORDINATES: protein motif:HMM:NF013245.2"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_component: GO:0016020 - membrane [Evidence IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="ABC transporter permease"
                     /protein_id="HOQ53465.1"
                     /translation="MSLADYATEHGLHRVGARPKLGQYLKQTWDRRDFAIAMARFRIQ
                     AAQETNRLGMVWLVLQPTLNALIYGLIFYFLQTGGNRPADYPAYIVIGVFLFEYFSKS
                     LNNGAKSITGNRGLVQSLSFPRLALPVAVVIENFMQLVPMMGVMAIFLIAMSHFPTWE
                     WLLMVPLIAMYTLFNAGIAMFAARLTVHVRDLTQILPFVNRILFYTSGVLFAVDKIFA
                     SHPWAIRLYDFHPIYQVLQIARGLLMHQGHYELHYWLYFALWSVLVFVGGLLFFWVAE
                     ERYGRD"
     gene            1045..1839
                     /locus_tag="PLF56_07615"
     CDS             1045..1839
                     /locus_tag="PLF56_07615"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_007076964.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_function: GO:0005524 - ATP binding [Evidence IEA];
                     GO_function: GO:0016887 - ATP hydrolysis activity
                     [Evidence IEA];
                     GO_function: GO:0042626 - ATPase-coupled transmembrane
                     transporter activity [Evidence IEA];
                     GO_function: GO:0140359 - ABC-type transporter activity
                     [Evidence IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="ABC transporter ATP-binding protein"
                     /protein_id="HOQ53466.1"
                     /translation="MAETDQPSGRTPHDTASADRQPVVVVDDLHVKYRVFAGGRAAEG
                     NAAASGLLKRTRGIRTVHALKGVTFTAYQNESIGIIGHNGSGKSTLMRAITGLTPPES
                     GAVYASSRPNLLGVGAALLPDLSGENNIILGGLALGFSKREVRDLRDDIVAFAELEQF
                     IDLPMRTYSSGMQARLKFSIATAKQHEILIVDEALSVGDKGFRARSEGRIREIRENAG
                     TVFLVSHSMGSIRDTCERTIWIHRGELRMDGPTDEVVGVYEDSKGR"
     gene            complement(1815..3362)
                     /gene="mptB"
                     /locus_tag="PLF56_07620"
     CDS             complement(1815..3362)
                     /gene="mptB"
                     /locus_tag="PLF56_07620"
                     /inference="COORDINATES: protein motif:HMM:NF038066.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="polyprenol phosphomannose-dependent alpha 1,6
                     mannosyltransferase MptB"
                     /protein_id="HOQ53467.1"
                     /translation="MHHTHHTQPSSRLTPDAPRAAWVGLIGSIAIAAGRLAWLADADD
                     KQATWWTARIRATLGENTAGPLTVLGACLLLLAWMLLRTELGRPRRLAITFVCWVLPL
                     LPLGGIMSSDPRYYADIGWSINHGHDPYSTGLGTTGSPFPVGPTWHGTFAVYPALALR
                     LFGWVVAVSGSDWYWSVVAMRALALAGVALLALSLPVLADQAGVDRRFVLWLGVFNPV
                     TMLHGIGGAHIDMLMVGMTAAALALAVRYRSLIIGALAVGVAAAIKQPALLAAVPVAL
                     LTAPVTQRTWPRTIVRTAASVALAIASLVAVSWLTGFGMGWLRAADTTYSIHTVGVGY
                     LIGEAVRMGATALGLPTSAAFVDVANTVVGALGYLLIVVLYFRYRHRPWQFLASAALT
                     WAIANGAFREWYLIYWLAFVALADLGPWMRRAVSVLVPFAMAYSAFKSYLGWNIMPAT
                     YLCAAIALAANARGWLLEAQDSGQRPMGTDDTATLTDADPEPKAEPAPESEPAEAIAA
                     QRPLESS"
     gene            complement(3422..>4920)
                     /locus_tag="PLF56_07625"
     CDS             complement(3422..>4920)
                     /locus_tag="PLF56_07625"
                     /inference="COORDINATES: protein motif:HMM:NF012271.2"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_function: GO:0005515 - protein binding [Evidence IEA]"
                     /codon_start=3
                     /transl_table=11
                     /product="fibronectin type III domain-containing protein"
                     /protein_id="HOQ53468.1"
                     /translation="LGADVVKLSWNAVSKAVKYKVRYSTSSKMTSATTVTQAESKDIT
                     TAFTNVSGLSANKTYYFQVVGIDSAGKDLNTWGNTSSGAKTFYSYATPTGVAAANIAS
                     TWVELSWQPVSGAPGYKVRYYNATDKARYVWNQVENFTLHGRNDSDNLRKNTKYSISV
                     AVQQPAIGTFGTSAYTPLVTMSPLSKEITLTTSNYAIAAPTNLVASQQQPTSVRMSWD
                     APSGVTDGMKYRVQYSTSSAMTGAKSVDTTATAVTLSKLSENTTYYVRVMLIDAAGAQ
                     ASDRSAFQIGKTRVSRGTIKGTVTGAPGRDVEAMVYGTGGEMVAQADVASNGSYSVSV
                     RPGSYKVLLSYVGTAGYTSMWAASGKSGGVPVMSQGSSFRVSGAGSSLTAPAVSLAKG
                     ASLSGTIKYNGTGLNGVFVTSLSGHTTAREVELVTQTPTSGGGVGFYSLKGLSEGQHW
                     LRFTRSGYKNVSIWIKVSGAVVTQYRVSTQTNPTNLAAGKALNLNMSK"
BASE COUNT          785 a         1605 c         1677 g          853 t
ORIGIN      
        1 aacgccccgg cagcccaggg tggtgcgcgc agtagcgcag ccccctgggc tgtgcgccag
       61 tccgtacgcc gggcgcacgg ctggcgcggg ctggtcgaag cgaacgccgg gcatgcctcg
      121 gcgcgggctg gtcgggcgga acgcatggca tggctagaat cagcgtgttt ggccccaggg
      181 gcacgcgcgc gagaggaacc cgaacgtcta tgagcttggc cgactacgcc acggagcacg
      241 ggctccaccg cgtcggcgca cgtcccaagc tggggcagta cctcaagcag acgtgggacc
      301 ggcgcgactt cgccatcgcg atggcccgct ttcgcatcca ggccgcgcaa gagaccaacc
      361 ggctcggcat ggtgtggctg gtgctgcagc ccacgctgaa cgcgctgatc tacggcctga
      421 tcttctattt tctgcagacc ggcggcaacc gtccggccga ctacccggcc tacatcgtga
      481 tcggcgtctt tctgttcgag tacttcagca agtcgctcaa caacggtgcc aaatcgatca
      541 ccggcaaccg cgggcttgtg cagtcgctgt cgtttccacg gctcgcgctg ccggtggcgg
      601 tggtgatcga gaacttcatg cagttggtgc cgatgatggg tgtgatggcg atctttctga
      661 tcgcgatgag ccactttccc acctgggagt ggctgttgat ggtgccgctg atcgcgatgt
      721 acacgctgtt caacgccggc atcgccatgt tcgcggcacg gttgaccgtg catgtgcgcg
      781 acctcaccca gattctgccg ttcgtcaacc gcatcctgtt ctacacctcg ggcgtgctgt
      841 tcgcggtcga caagatcttc gcctcgcacc cgtgggcgat ccggctctac gactttcacc
      901 cgatctacca ggtgctgcag atcgcccgcg ggctgctgat gcaccagggc cactacgagc
      961 tgcactactg gctgtatttc gccttgtggt ccgtgctggt cttcgtgggc ggcctgctgt
     1021 tcttctgggt ggccgaggag cgctatggcc gagactgacc agccgagcgg ccgcacgccg
     1081 cacgacaccg cgtcggccga tcggcaaccg gtggtggtgg tggacgacct gcacgtcaag
     1141 taccgggtgt tcgccggcgg acgggcggcc gagggcaacg cggcggcgtc cggactgttg
     1201 aagcgaacgc gcggcatccg caccgtccat gcgctcaagg gcgtgacgtt caccgcctac
     1261 cagaacgagt cgatcggcat catcgggcac aacggctcgg gcaagtcgac gctgatgcgg
     1321 gccatcaccg gcctgacgcc acccgagagc ggggcggtct acgcgtcgtc ccggcccaac
     1381 ctgctggggg tcggcgcggc gctgctaccc gacctgtcgg gcgagaacaa catcattctg
     1441 ggcggcctcg cccttggctt cagcaagcgc gaggtgcgcg acctgcgcga cgacatcgtg
     1501 gcgttcgccg aactggagca gttcatcgac ctgccgatgc gcacctactc ctcgggcatg
     1561 caggcgcggt tgaagttctc gatcgccacc gcgaagcagc acgagatcct gatcgttgac
     1621 gaggcgttgt ccgtgggtga caagggcttt cgggcccgaa gcgagggccg catccgcgag
     1681 atccgcgaga acgccggcac ggtgttcctg gtttcgcact cgatggggtc gatccgcgac
     1741 acctgcgagc gcacgatctg gatccaccgc ggcgagttgc gtatggacgg gccgaccgac
     1801 gaggtggtcg gtgtctacga ggactccaag ggtcgctgag cggcgatcgc ttcggcgggt
     1861 tctgattctg gtgcgggttc cgctttcggt tcgggatcgg cgtcagtgag cgttgccgtg
     1921 tcgtccgtgc ccatgggtcg ctggccggag tcctgcgcct ctagtagcca gccgcgcgcg
     1981 ttggccgcca gagcgatcgc cgcgcacagg tacgtggccg gcatgatgtt ccagcccaga
     2041 tacgacttga acgcgctgta ggccatggcg aagggcacca gcacgctgac cgcccgacgc
     2101 atccagggac cgaggtcggc gagggcgacg aaggccagcc aatagatcag gtaccactcc
     2161 cggaacgccc cgttggcgat cgcccaggtc agggccgcgg acgccagaaa ctgccagggc
     2221 cgatgccggt agcgaaagta gagtacgacg atcagcaggt aacccagggc gccgaccacc
     2281 gtattggcaa cgtcgacaaa ggccgccgac gtgggcagtc cgagggcggt cgcgcccatc
     2341 cggacggcct cgccgatcag gtagccgacc ccgacggtgt gaatggagta ggtggtgtcg
     2401 gcggctcgca gccagcccat accgaatccc gtcagccaac tcaccgcgac cagcgaagcg
     2461 attgcgagcg ccaccgaggc cgccgtccga acaatggtgc gcggccaggt gcgctgcgtc
     2521 accggtgcgg tcagcagggc caccggcacg gccgccaaca gggcgggttg cttgattgcg
     2581 gcggcaacgc cgacggccag tgcaccgatg atcaggctgc ggtaccgcac cgccagcgcc
     2641 aacgccgcag cggtcatgcc caccatcaac atgtcgatat gggcacctcc gatgccgtgc
     2701 agcatcgtca ctggattgaa cacaccgagc cacaggacga accggcgatc caccccggcc
     2761 tgatctgcca gcaccggcag gctcagcgcc agcagggcga cgccggccag cgccagcgcc
     2821 cgcatcgcga ccaccgacca gtaccagtcg gatccggaca ccgccaccac ccagccgaac
     2881 agccgcagtg ccagcgccgg atacaccgcg aacgtgccgt gccaggtggg tccgaccgga
     2941 aacgggctgc ccgtggtgcc caggccggtg ctgtaggggt catgcccgtg gttgatcgac
     3001 cagccgatgt cggcgtagta gcgggggtcg gacgacatga tgccgcccag cggcaacagt
     3061 ggcagcaccc agcagacgaa cgtgatcgcc agccggcgcg gccggccgag ttcggtgcgc
     3121 agcagcatcc aggccagcag cagcaggcaa gcccccagca cggtgagcgg gcccgccgtg
     3181 ttctcgccca gagtggcccg aatgcgagca gtccaccagg tggcctgttt gtcgtccgcg
     3241 tcggccagcc aggcaagccg tccggcggcg atggcgatcg agccgatcag ccccacccag
     3301 gccgcccggg gggcgtcagg tgtgaggcga ctcgacggct gtgtgtggtg cgtgtggtgc
     3361 atggtggtga tgagctttgc agagaatctg cgccctgcga ccggacctcg cctctaccgg
     3421 gctacttgct catgttgagg ttgagggctt tgcctgcggc caggttcgtc gggttggttt
     3481 gcgtggacac ccggtactgg gtgacgaccg caccggagac cttgatccag atgctcacgt
     3541 tcttgtagcc cgagcgggtg aacctcagcc agtgctggcc ttcggagagc cccttgaggc
     3601 tgtagaagcc gacaccgcca ccgctggtcg gggtctgggt gaccagttcg acctctcgcg
     3661 cggtggtgtg cccgctcagg ctggtgacga atacgccgtt gaggccggtg ccgttgtact
     3721 tgatcgtgcc cgacagggag gcgcccttcg ccaggctcac cgcgggcgcc gtgaggctcg
     3781 aaccggcgcc ggacacacgg aagctgctgc cctgcgacat caccggtacg ccgccgctct
     3841 tgccgctggc cgcccacatg gaggtgtagc cggcggtgcc cacgtagctg agcagcacct
     3901 tgtagctgcc cgggcgcacc gacaccgaat aactgccgtt cgaggccaca tcggcctggg
     3961 cgaccatctc gccacctgtg ccgtacacca tcgcctcgac gtcacggcca ggcgcgccgg
     4021 tcacggtgcc cttgatggtg cctcggctga cccgggtctt gccgatctgg aaggcggagc
     4081 ggtcgctggc ctgcgcgccc gcggcgtcga tcagcatcac ccgcacgtaa taggtggtgt
     4141 tctcgctgag cttgctgagc gtcaccgccg tcgcggtggt gtcgaccgac ttggccccgg
     4201 tcatggccga actcgtcgag tactgcaccc ggtacttcat gccgtcggtg acgccgctgg
     4261 gggcgtccca gctcatccgg acgctggtcg gctgctgctg gctggcgacc aggttggtgg
     4321 gtgctgcgat cgcatagttg gacgtggtga gcgtgatctc tttggacagg gggctcatgg
     4381 tcaccagcgg cgtgtacgcg gacgtgccga aggtgccgat cgctggctgc tgcactgcga
     4441 cgctgatcga gtacttggtg ttcttgcgca ggttgtcgct gtcgttacgg ccgtgcaggg
     4501 tgaagttctc gacctggttc cacacgtagc gggccttgtc ggtggcgttg tagtaacgca
     4561 ccttgtaacc gggtgcgccc gataccggtt gccagctcaa ctcgacccac gtgcttgcga
     4621 tgttggcggc ggccaccccg gtcggcgtgg catagctgta gaaggtcttg gcgcccgagg
     4681 aggtgttgcc ccacgtgttg aggtctttgc cggcgctgtc gatgccgacg acctgaaagt
     4741 agtaggtctt gttcgccgac aggcccgaca cgttggtgaa cgccgtggtg atgtctttgc
     4801 tctccgcctg ggtcacggtg gtcgcactgg tcatcttgga cgaggtggag tagcgcacct
     4861 tgtacttcac ggccttgctc accgcgttcc acgagagctt caccacatcg gcgccgagcg
//
LOCUS       DAOSUG010000201         4901 bp    DNA     linear   ENV 10-JUN-2024
DEFINITION  MAG TPA_asm: Micropruina sp. isolate AMR_MDS_2195
            NODE_7561_length_4901_cov_3.948411, whole genome shotgun sequence.
ACCESSION   DAOSUG010000201 DAOSUG010000000
VERSION     DAOSUG010000201.1
DBLINK      BioProject: PRJNA925477
            BioSample: SAMN32864262
            Sequence Read Archive: SRR4236673
KEYWORDS    WGS; ENV; Metagenome Assembled Genome; MAG; Third Party Data; TPA;
            TPA:assembly.
SOURCE      Micropruina sp. (wastewater metagenome)
  ORGANISM  Micropruina sp.
            Bacteria; Bacillati; Actinomycetota; Actinomycetes;
            Propionibacteriales; Nocardioidaceae; Micropruina.
REFERENCE   1  (bases 1 to 4901)
  AUTHORS   Abdulkadir,N., Saraiva,J.P., Zhang,J., Stolte,S., Gillor,O.,
            Harms,H. and Rocha,U.
  TITLE     Genome-centric analyses of 165 metagenomes show that mobile genetic
            elements are crucial for the transmission of antimicrobial
            resistance genes to pathogens in activated sludge and wastewater
  JOURNAL   Microbiol Spectr 12 (3), e0291823 (2024)
   PUBMED   38289113
REFERENCE   2  (bases 1 to 4901)
  AUTHORS   Abdulkadir,N.
  TITLE     Direct Submission
  JOURNAL   Submitted (24-MAR-2023) Environmental Microbiology, Helmholtz
            Center for Environmental Research -UFZ, Permoserstr 15, Leipzig
            04318, Germany
COMMENT     The annotation was added by the NCBI Prokaryotic Genome Annotation
            Pipeline (PGAP). Information about PGAP can be found here:
            https://www.ncbi.nlm.nih.gov/genome/annotation_prok/
            
            ##Genome-Assembly-Data-START##
            Assembly Method        :: SPAdes v. 3.11.1
            Genome Representation  :: Full
            Expected Final Version :: Yes
            Genome Coverage        :: Not Applicable
            Sequencing Technology  :: Illumina MiSeq
            ##Genome-Assembly-Data-END##
            
            ##Genome-Annotation-Data-START##
            Annotation Provider               :: NCBI
            Annotation Date                   :: 05/04/2023 04:57:10
            Annotation Pipeline               :: NCBI Prokaryotic Genome
                                                 Annotation Pipeline (PGAP)
            Annotation Method                 :: Best-placed reference protein
                                                 set; GeneMarkS-2+
            Annotation Software revision      :: 6.5
            Features Annotated                :: Gene; CDS; rRNA; tRNA; ncRNA
            Genes (total)                     :: 2,776
            CDSs (total)                      :: 2,750
            Genes (coding)                    :: 2,714
            CDSs (with protein)               :: 2,714
            Genes (RNA)                       :: 26
            tRNAs                             :: 24
            ncRNAs                            :: 2
            Pseudo Genes (total)              :: 36
            CDSs (without protein)            :: 36
            Pseudo Genes (ambiguous residues) :: 0 of 36
            Pseudo Genes (frameshifted)       :: 2 of 36
            Pseudo Genes (incomplete)         :: 32 of 36
            Pseudo Genes (internal stop)      :: 2 of 36
            ##Genome-Annotation-Data-END##
FEATURES             Location/Qualifiers
     source          1..4901
                     /organism="Micropruina sp."
                     /mol_type="genomic DNA"
                     /submitter_seqid="NODE_7561_length_4901_cov_3.948411"
                     /isolate="AMR_MDS_2195"
                     /isolation_source="Activated sludge of municipal
                     wastewater treatment plants"
                     /db_xref="taxon:2737536"
                     /environmental_sample
                     /geo_loc_name="Canada"
                     /lat_lon="49.25 N 123.1 W"
                     /collection_date="2015-06-16"
                     /metagenome_source="wastewater metagenome"
                     /note="metagenomic"
     gene            <1..1170
                     /gene="aspS"
                     /locus_tag="PLF56_07630"
     CDS             <1..1170
                     /gene="aspS"
                     /locus_tag="PLF56_07630"
                     /EC_number="6.1.1.12"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_008356851.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_function: GO:0000166 - nucleotide binding [Evidence
                     IEA];
                     GO_function: GO:0004812 - aminoacyl-tRNA ligase activity
                     [Evidence IEA];
                     GO_function: GO:0005524 - ATP binding [Evidence IEA];
                     GO_function: GO:0016874 - ligase activity [Evidence IEA];
                     GO_process: GO:0006418 - tRNA aminoacylation for protein
                     translation [Evidence IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="aspartate--tRNA ligase"
                     /protein_id="HOQ53469.1"
                     /translation="MVAGMERYYQIARCYRDEDFRADRQPEFTQLDIEMSFVQQDDVI
                     ELGEQLAREIWALIGVELTTPFPRLTYAEAMDAYGSDKPDLRFEVKLTDLTTFFADTP
                     FRVFQAPYVGAVVMPGGASQPRRTFDAWQEWAKQRGARGLAYVTIAENGELGGPVSKN
                     ISETERAGLAAAVGANPGDCVFFAAGARSTSQALLGAARNEIAKRCELIDEGSWSFLW
                     VVDAPLFKPTGEAVAEGDVAVGGGAWTAVHHAFTSPQDLTTFDTDPGNALAWAYDMVC
                     NGNEIGGGSIRIHRRDIQERVFKVMGLTEDEAQEKFGFLLDAFKFGAPPHGGIAFGWD
                     RIVALLSGSESIRDVIAFPKSGGGYDPLTGAPAPISPAQRKEAGVDAKPAAPATA"
     gene            complement(1706..2221)
                     /locus_tag="PLF56_07635"
     CDS             complement(1706..2221)
                     /locus_tag="PLF56_07635"
                     /inference="COORDINATES: protein motif:HMM:NF015513.2"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_function: GO:0003677 - DNA binding [Evidence IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="PadR family transcriptional regulator"
                     /protein_id="HOQ53470.1"
                     /translation="MGPFGGPMGRRGRGRARRGDVRQAVLALLAEQPLNGYQMIQTLA
                     ERTEGGWKPSPGAIYPALAQLEDEGLIEQFDNEGTKAYRLTDAGRTAAAEISTKPWDA
                     FNTENAPAHPEAIKAMWSEFRGLAMAAQELTRNGSKAQQENAGQLLADTKRKLYGLLA
                     DGDVHNGDDLR"
     gene            2567..3919
                     /locus_tag="PLF56_07640"
     CDS             2567..3919
                     /locus_tag="PLF56_07640"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_002526819.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_function: GO:0005524 - ATP binding [Evidence IEA];
                     GO_process: GO:0006260 - DNA replication [Evidence IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="replication-associated recombination protein A"
                     /protein_id="HOQ53471.1"
                     /translation="MEDLFGNDDAPAAPGGSLSTPSADAPLAVRMRPRTVDEIVGQQH
                     LLGAGSPLRRLAGGDAAMSVFLWGPPGVGKTTIAAVVSQSAKRRFVEISAVTAGVKEV
                     RAELETAKRELARGRATVLFVDEVHRFNKAQQDVLLPAVEKRLVTLIAATSENPSFSV
                     IAPLLSRSLLLTLKPLTDADLGVLIDRALHDQRGLAGRIALSDEARADLLRLAGGDAR
                     RALTYLEESAAGASAEGLTEITPEQISRAVDRAAVRYDRDGDQHYDVISAFIKSLRGS
                     DVQAALHYLARMIEAGEDPRFIARRLVILASEDIGMAAPSVLQTCVAAAQAVQLIGMP
                     EARLNLAQAVIACAMAPKSNAVIMAMDAALADVRAGRIGQVPPHLRDAHYAGAKTLGH
                     GKGYKYAHDHPHGVAAQQYLPDELDGTRYYRPTDHGNEAAMMERLATLDELLGRPTNP
                     "
     gene            4008..>4901
                     /locus_tag="PLF56_07645"
     CDS             4008..>4901
                     /locus_tag="PLF56_07645"
                     /inference="COORDINATES: protein motif:HMM:NF018584.2"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="DUF1266 domain-containing protein"
                     /protein_id="HOQ53472.1"
                     /translation="MTYVQNHIGDLTYPGEKYFHPDWAQARLDPRTKLATSWILIVAA
                     LSLAVLLLCAINYGGTPARGRAGLLTFMGIAAVVVVVLGTWGWRRYQATRSSLWRAQV
                     DYNTVVGRTAPTPEQLEALQIGSVWDYRDKAWGHSLAKFPCEREVAHVPQWQRRFAML
                     DPGSLTDHRAALMEWWGIGTATSYRQMVQRLFGGLHSRDLDEAYLQAADVPALIDHLA
                     GRSDLPAAYIDECIHGSLQQRGVRPPARLWGWDLGRISIIARGAYGAGLIDADEAWRD
                     IALASEWVRTIFPTCEDFFRNV"
BASE COUNT          745 a         1694 c         1693 g          769 t
ORIGIN      
        1 atggtggccg gcatggagcg ctactaccag atcgcccgct gctaccgcga cgaggacttc
       61 cgcgccgacc ggcagcccga gttcacccag ctcgacatcg agatgagctt cgtccagcag
      121 gacgacgtga tcgaactcgg tgaacaactg gcgagagaga tctgggcgct gatcggcgtc
      181 gagctcacga ctccgttccc gcggctcacc tacgccgagg cgatggacgc ctacggctcc
      241 gacaagcccg acctgcgctt cgaggtgaag ctcaccgacc tgaccacgtt cttcgccgac
      301 accccgttcc gggtcttcca ggccccctac gtgggtgccg tggtgatgcc cggtggtgcg
      361 tcccagccgc gtcgtacctt cgacgcctgg caggagtggg ccaagcagcg cggtgctcgc
      421 gggctggcct acgtgacgat cgccgaaaac ggcgaactgg gcggtccggt cagcaagaac
      481 atctccgaga ccgagcgcgc ggggcttgcc gcggccgtcg gcgcgaaccc cggtgattgc
      541 gtcttcttcg cggccggtgc caggtcgacg tcccaggcgt tgctgggcgc ggcccgcaac
      601 gagatcgcca agcgctgcga gctgatcgac gagggctcct ggtcgttcct gtgggtggtg
      661 gacgctccgc tgttcaagcc caccggcgag gccgttgccg agggtgacgt ggcggtcggc
      721 ggtggcgcgt ggacggccgt ccaccacgcc ttcacctcgc cgcaggatct gaccaccttc
      781 gacaccgatc ccggcaacgc gctggcctgg gcctacgaca tggtctgcaa cggcaatgag
      841 atcggcggcg gctcgatccg tattcatcgc cgcgacatcc aggagcgggt gttcaaggtg
      901 atggggctga ccgaggacga ggcgcaggag aagttcggct ttctgctgga cgccttcaag
      961 ttcggggcgc cgccgcacgg cggcatcgcc ttcggctggg accggatcgt ggccctgctg
     1021 tcgggttcgg agtcgatccg cgacgtgatc gccttcccga agagcggcgg cggctacgac
     1081 ccgttgaccg gcgccccggc accgatcagc ccggcacaac gtaaggaggc cggggtggac
     1141 gccaagccgg cggcacccgc gactgcctga ccggacggga tcccggcgtg cgccgggatg
     1201 tcgggcctgc gggggattgc tcgcacgccg cgacgacagg ccggcgccga gcagccaatg
     1261 gtgacgagcg cgactgcctg accggacggg atcccggcgt gcgccgggat gacagggcct
     1321 ttgcacacaa cgccgggtgt acgcgaaggc ggcggctccc gtgactgtcc gaccggacgg
     1381 gagccgggat gacaggtcgc ctcaggcgag ggtttcaggc cgctacccaa gagtgctggt
     1441 aggcgggccc ctgggaccga ccgctacgac ctgcagaccc gcgagctgct cgagtggtca
     1501 gtttgggatc cggacgaccg cggacggcag tcatcgggtt cacctgcctc ggctctgagg
     1561 tggccgccga ggcgccgcgc tgagcgttga tcagagcgta tgtctcccgg caccaggtgt
     1621 ccggccgcgc gggtcgaggc gtggggtgcc agtttgcacg aaaggccgcc cgatcactcg
     1681 gacggccctt cggggctggc tgcgctcagc gcagatcgtc gccgttgtgc acgtcgccgt
     1741 cggccagcag gccgtacagc ttgcgcttgg tatcggccag cagttgaccc gcgttctcct
     1801 gctgggcctt gctgccgttg cgggtcagct cctgggcggc cattgccagc ccacggaact
     1861 cgctccacat ggccttgatg gcctcgggat gagccggcgc gttctcggta ttgaaagcat
     1921 cccaaggctt ggtgctgatc tcagcggcag cagtgcgccc cgcgtcggtc agccggtagg
     1981 ccttggtgcc ctcgttgtcg aactgctcga tcagaccctc gtcctcgagc tgcgccagag
     2041 ccgggtagat ggcgccgggg gagggcttcc agccaccctc ggtgcgctcg gccagcgtct
     2101 ggatcatctg atacccgttg agcggctgct cggcgagcag ggccagaacg gcctgccgca
     2161 cgtcgccgcg ccgggcgcgt ccgcgcccac ggcgacccat cggcccgcca aacggaccca
     2221 ttgggccacc gaacgggcct ggcccgccga agccgggacc accgaagcca ccgccgaagc
     2281 cgccgcgtcc gtggccttcg ccgaatgccc cgcggtgggg cctccgaccg tgtcgctgcc
     2341 cgtcattggt gagcgggcca tccgagttga agtcttggtt cctcatggtg ggaactcctt
     2401 tcgtacgtct tacgttgcat caacgatata tcgcgagtgt tcgcaatgca agtcgtgacg
     2461 aaaggtgcgc gccaacggcg gcagctgcgg cgtgtcgcgg ccgtcccggc gcactggcgc
     2521 gcacttttcg tacaacgcgt cagcgcccac ccgtacactc ggcgcggtgg aggatctgtt
     2581 cggcaacgat gacgccccgg ccgcaccggg agggtcgctg agcacaccct ccgcagacgc
     2641 acccctcgcg gtccgcatgc gcccgcgcac ggtggacgag atcgtcggcc agcagcatct
     2701 gctcggtgcc ggttcaccgc tacgcaggct ggccggtggc gatgcggcca tgtcggtgtt
     2761 tctgtggggt ccgcccgggg tcggcaagac gaccatcgcc gccgtcgtct cgcagtcggc
     2821 gaagcgtcgc ttcgtcgaga tctcggccgt caccgccggc gtcaaagagg tgcgggccga
     2881 actcgagacc gccaaacgcg agctggctcg cggacgggcg accgtgctgt tcgtggacga
     2941 ggtgcaccgg ttcaacaagg cccagcagga cgtgctgctg cccgcggtcg agaaacgcct
     3001 ggtcacgctg atcgccgcga cctccgaaaa cccgtcgttc tcggtgatcg cgccgctgct
     3061 gtcccgctcg ctgctgttga ccctcaagcc gctcaccgat gccgatctgg gcgtcttgat
     3121 cgaccgcgcc ctgcacgacc agcgtggcct ggccggacgg atcgcgctca gcgacgaggc
     3181 ccgtgccgat ctgctgcggc tggccggcgg ggacgcccgg cgggccctca cctacctgga
     3241 ggagtcggcc gcgggtgcgt ccgccgaggg gctgacagag atcacccccg agcagatcag
     3301 ccgcgccgtc gaccgagccg ccgtgcgtta cgaccgcgac ggcgatcagc actacgacgt
     3361 gatcagtgcg ttcatcaagt cactgcgcgg ctcggacgtc caggccgcgc tgcactacct
     3421 ggcccgcatg atcgaggcgg gcgaggatcc gcggttcatc gcccgacgcc tggtgatcct
     3481 cgccagcgaa gacatcggaa tggcggcccc ctcggtgctg cagacctgtg tcgcggcagc
     3541 gcaagccgtc cagctgatcg gcatgcccga ggctcggctc aacctcgccc aagccgtcat
     3601 cgcctgcgcg atggcgccca agtcgaacgc ggtgatcatg gccatggacg ccgccctggc
     3661 cgacgtgcgc gccggacgca tcggccaggt gcccccgcat ctgcgcgacg cccactacgc
     3721 cggcgcgaag acgttgggcc acggcaaggg ctacaagtac gcccacgacc acccgcacgg
     3781 ggtggcggcc cagcaatacc tacccgacga actggacggc acccgctact accggcccac
     3841 cgaccacggc aacgaggctg cgatgatgga gcgcctggcg actctggacg agttgctcgg
     3901 gcgcccgacg aacccgtagt gccgtgcggc ccgctccgcg ccgtggcgag tgcagccccc
     3961 gcccggttga atgagttgat cccgacgacc gaaggaaccg aaccggcgtg acctacgtgc
     4021 agaaccacat tggcgacctg acctatccgg gcgagaagta ctttcacccc gactgggcac
     4081 aggctcggct cgatccccgc acgaagctgg cgacgagctg gatcctgatc gttgccgcgc
     4141 tgagcctggc cgtccttctg ctgtgcgcga tcaactatgg cggcacgccc gcgcgcggcc
     4201 gtgccggact gttgacattc atggggatcg cggccgtggt ggtagtggtg ctcggaacgt
     4261 ggggatggcg ccgctaccag gcgacgcgct cgagcctgtg gcgggcccag gtcgactaca
     4321 acaccgtggt cgggcgaacc gcgccgacgc ccgagcaatt ggaggctctg cagatcggat
     4381 ccgtctggga ctaccgcgac aaggcgtggg ggcactcgct ggccaaattt ccctgcgagc
     4441 gcgaggtcgc acacgtgccg cagtggcagc gccgcttcgc catgctcgat ccgggatcgc
     4501 tgaccgatca ccgcgccgcc ctgatggagt ggtggggcat cggcacggcc acgtcgtacc
     4561 ggcagatggt gcagcgactg ttcggtggcc tgcactcgcg cgatctcgac gaggcctacc
     4621 tccaagccgc cgacgtgccg gccctcatcg atcacctggc cggacgctcc gatctgccgg
     4681 cggcctacat cgacgaatgc atccacggct cgttgcagca gcgcggggtg cgtccaccgg
     4741 cgcggctgtg ggggtgggat ctcggccgca tctcgatcat cgccaggggt gcgtacgggg
     4801 cggggctgat cgacgccgac gaggcgtggc gcgacattgc cttggcctcg gagtgggtgc
     4861 gcacgatctt ccccacctgc gaggacttct ttcgcaacgt c
//
LOCUS       DAOSUG010000202         4900 bp    DNA     linear   ENV 10-JUN-2024
DEFINITION  MAG TPA_asm: Micropruina sp. isolate AMR_MDS_2195
            NODE_7565_length_4900_cov_3.861094, whole genome shotgun sequence.
ACCESSION   DAOSUG010000202 DAOSUG010000000
VERSION     DAOSUG010000202.1
DBLINK      BioProject: PRJNA925477
            BioSample: SAMN32864262
            Sequence Read Archive: SRR4236673
KEYWORDS    WGS; ENV; Metagenome Assembled Genome; MAG; Third Party Data; TPA;
            TPA:assembly.
SOURCE      Micropruina sp. (wastewater metagenome)
  ORGANISM  Micropruina sp.
            Bacteria; Bacillati; Actinomycetota; Actinomycetes;
            Propionibacteriales; Nocardioidaceae; Micropruina.
REFERENCE   1  (bases 1 to 4900)
  AUTHORS   Abdulkadir,N., Saraiva,J.P., Zhang,J., Stolte,S., Gillor,O.,
            Harms,H. and Rocha,U.
  TITLE     Genome-centric analyses of 165 metagenomes show that mobile genetic
            elements are crucial for the transmission of antimicrobial
            resistance genes to pathogens in activated sludge and wastewater
  JOURNAL   Microbiol Spectr 12 (3), e0291823 (2024)
   PUBMED   38289113
REFERENCE   2  (bases 1 to 4900)
  AUTHORS   Abdulkadir,N.
  TITLE     Direct Submission
  JOURNAL   Submitted (24-MAR-2023) Environmental Microbiology, Helmholtz
            Center for Environmental Research -UFZ, Permoserstr 15, Leipzig
            04318, Germany
COMMENT     The annotation was added by the NCBI Prokaryotic Genome Annotation
            Pipeline (PGAP). Information about PGAP can be found here:
            https://www.ncbi.nlm.nih.gov/genome/annotation_prok/
            
            ##Genome-Assembly-Data-START##
            Assembly Method        :: SPAdes v. 3.11.1
            Genome Representation  :: Full
            Expected Final Version :: Yes
            Genome Coverage        :: Not Applicable
            Sequencing Technology  :: Illumina MiSeq
            ##Genome-Assembly-Data-END##
            
            ##Genome-Annotation-Data-START##
            Annotation Provider               :: NCBI
            Annotation Date                   :: 05/04/2023 04:57:10
            Annotation Pipeline               :: NCBI Prokaryotic Genome
                                                 Annotation Pipeline (PGAP)
            Annotation Method                 :: Best-placed reference protein
                                                 set; GeneMarkS-2+
            Annotation Software revision      :: 6.5
            Features Annotated                :: Gene; CDS; rRNA; tRNA; ncRNA
            Genes (total)                     :: 2,776
            CDSs (total)                      :: 2,750
            Genes (coding)                    :: 2,714
            CDSs (with protein)               :: 2,714
            Genes (RNA)                       :: 26
            tRNAs                             :: 24
            ncRNAs                            :: 2
            Pseudo Genes (total)              :: 36
            CDSs (without protein)            :: 36
            Pseudo Genes (ambiguous residues) :: 0 of 36
            Pseudo Genes (frameshifted)       :: 2 of 36
            Pseudo Genes (incomplete)         :: 32 of 36
            Pseudo Genes (internal stop)      :: 2 of 36
            ##Genome-Annotation-Data-END##
FEATURES             Location/Qualifiers
     source          1..4900
                     /organism="Micropruina sp."
                     /mol_type="genomic DNA"
                     /submitter_seqid="NODE_7565_length_4900_cov_3.861094"
                     /isolate="AMR_MDS_2195"
                     /isolation_source="Activated sludge of municipal
                     wastewater treatment plants"
                     /db_xref="taxon:2737536"
                     /environmental_sample
                     /geo_loc_name="Canada"
                     /lat_lon="49.25 N 123.1 W"
                     /collection_date="2015-06-16"
                     /metagenome_source="wastewater metagenome"
                     /note="metagenomic"
     gene            complement(157..1254)
                     /locus_tag="PLF56_07650"
     CDS             complement(157..1254)
                     /locus_tag="PLF56_07650"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_008363279.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="Rossmann fold nucleotide-binding protein"
                     /protein_id="HOQ53473.1"
                     /translation="MRIDLESLDEFDAHLGATGSLAGVVVQSIDLTHRSAALRDAHVA
                     GAHFLGCTLRPADASFLVRAGALVFPPVPDLPFDPYRPTLYTADELYRGLDGGYPATL
                     DARIFAWSQQQLRPGELGADLAVTLHDHAISEALAESVAGIDPRRIVGIMGGHAQHRG
                     SRAYRASAQLAHHLADDGVVVLSGGGPGAMEAANLGAAFTGTGDELDTVIDTLAVAVA
                     RAQDLTSWARSAQQVRATQPCTRFSLGIPTWFYGHEPPNLFATGIAKYFTNALREDTL
                     LRLCRGGLVFMPGAAGTVQEVFQAVTPNFYARAGEPTVPLVMVGVQFWTTEVPAWPLL
                     QALADGRGMAASVHLVDTVDEVFAALGRACP"
     gene            1291..1830
                     /locus_tag="PLF56_07655"
     CDS             1291..1830
                     /locus_tag="PLF56_07655"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_015069382.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_function: GO:0032559 - adenyl ribonucleotide binding
                     [Evidence IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="universal stress protein"
                     /protein_id="HOQ53474.1"
                     /translation="MPQQVPQPDPSRMTPFTGGAIVAAVRPGSDPLVALTAADLARVT
                     GGTLYFAFVDEQRFVERELPDGSVRHADLNPDAGDDAWRRREAGMTADLRRLLADAGV
                     TWQFRYLAGRPDRALTHLARAVDAAVIVVGHHAPERGARVREFLDGALALKLAQHQHR
                     PVLAVPLRVVDWKAPMPWG"
     gene            1821..2228
                     /locus_tag="PLF56_07660"
     CDS             1821..2228
                     /locus_tag="PLF56_07660"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_015069383.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="CrcB family protein"
                     /protein_id="HOQ53475.1"
                     /translation="MGLSTARGATTLVVFAGGALGTATRAALEAANPAPQGGWPWATF
                     LINVSGAFVLGLLLELLSRRGPDVGARRLLRLGLGTGVMGGYTTYSTFALETVRLLEG
                     GALLLGLGYALGSVLLGLAAAWAGTLLAIRIAP"
     gene            2225..2590
                     /locus_tag="PLF56_07665"
     CDS             2225..2590
                     /locus_tag="PLF56_07665"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_016667332.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="CrcB family protein"
                     /protein_id="HOQ53476.1"
                     /translation="MIALLVALAGGLGAVARFHSDAFIARVNRLPLPLGTLVINTVGS
                     LLLGLFSGWVVFHSGAPAWAGIAGTGFCGGFTTFSTACVEGWRLVRAGRPVAAVLHAG
                     GGLVAGLLAASLGLWLMSL"
     gene            2741..3901
                     /gene="dxr"
                     /locus_tag="PLF56_07670"
     CDS             2741..3901
                     /gene="dxr"
                     /locus_tag="PLF56_07670"
                     /EC_number="1.1.1.267"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_020387240.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_component: GO:0005737 - cytoplasm [Evidence IEA];
                     GO_function: GO:0030604 - 1-deoxy-D-xylulose-5-phosphate
                     reductoisomerase activity [Evidence IEA];
                     GO_process: GO:0019288 - isopentenyl diphosphate
                     biosynthetic process, methylerythritol 4-phosphate pathway
                     [Evidence IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="1-deoxy-D-xylulose-5-phosphate reductoisomerase"
                     /protein_id="HOQ53477.1"
                     /translation="MRDVVLLGSTGSIGSQTLEVIEAHPEKFRVIGLSAGGSQLDTLI
                     AQAQAHRPDVVAVPTPAAAAALEGRLPADTAIWTGPDASARLAALPCDVVVNAITGAA
                     GLEPTLAALAAGNALALANKESLVIGGELVTAAAAPGQILAVDSEHSAFAQALRAGRS
                     DEVRRLILTASGGPFRGRTRDELTDVTPEQALAHPTWSMGRIITINSATLINKGLELL
                     EAALLYGVDLDAIEVVVHPSSDVHSMVEFHDGSTIAQCSPPDMKLPIALALNWPERLP
                     GASRPFDWSRPIDWHFEPLDSHAFPSVELARRCGRAGGTAPAVYNAANEICVDAFCER
                     RLGFLGIVDTVTAVVDEHLAGAANQAPMSVDSVLDADAWARRRAAELIGVAG"
     gene            3898..>4900
                     /locus_tag="PLF56_07675"
     CDS             3898..>4900
                     /locus_tag="PLF56_07675"
                     /inference="COORDINATES: protein motif:HMM:NF014244.2"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_function: GO:0004222 - metalloendopeptidase activity
                     [Evidence IEA];
                     GO_process: GO:0006508 - proteolysis [Evidence IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="site-2 protease family protein"
                     /protein_id="HOQ53478.1"
                     /translation="MNGLVTVVFAVVFFGCILLSIALHEIGHMLPAKRFGVRVPQYFV
                     GFGPTMWSTVRGETEYGIKWIPLGGFVRLLGMYPPGRTRTGQGNRLTRFADGARAYEW
                     EEITPDDVANERLFYQKKTWQKLVVMAGGPMMNVLIAFVLFWAILGLHGSYQAQPVVA
                     QIQQCIVRENQPADTPCPTPDTPAAKSGIKAGDTVVEFNGHQITDYAQLQGLIRANLD
                     GEARMVVERDGQRVSLTPVHTVISGVQDNLDPSKRIAAGWFGITPTTELVKGGPVETV
                     QQMWTMTTQSVVAITQFPVKVYNVVADLVQGKPRDIYGPLSIVGASRAAGEVVASDAI
                     "
BASE COUNT          681 a         1686 c         1754 g          779 t
ORIGIN      
        1 accagcaggc gttgcagacc gtctcggatc cccgctcggc aggacggagg gaggttccgc
       61 cacgctcaac ggacggactg atcagtcgcc ggtcgggctc gtcgagacct tgtgagtagg
      121 ggtttcagca cgctcgacga tcggacggtg cgttgctcag gggcatgcgc ggccgagggc
      181 cgcgaacacc tcatcgacgg tgtcgaccag gtgcacggac gcagccatac cccggccgtc
      241 ggccagggcc tgcagcagcg gccaggccgg cacctcggtc gtccagaact ggacgccgac
      301 catcaccagg ggcacagtcg gctcgcccgc gcgggcgtag aagttgggcg tgacggcctg
      361 gaacacctcc tgcacggtgc cggccgcgcc gggcatgaac accaggcccc cgcggcacag
      421 ccgtaacagg gtgtcttcgc gcagcgcgtt ggtgaagtac ttggcgatgc cggtggcgaa
      481 caggttgggc ggctcgtggc cgtagaacca ggtcggtatg ccgagcgaga accgggtgca
      541 cggctgcgtg gcgcgcacct gctgcgccga gcgtgcccat gaggtcagat cctgggcccg
      601 ggccacggcc acagcgagcg tgtcgatcac ggtgtcgagc tcatcgccgg tgccggtgaa
      661 tgccgccccg aggttggccg cctccatcgc gcccgggccg ccaccggaca gcaccaccac
      721 accgtcgtcg gccaggtgat gggccagctg cgcgctggcc cggtaggcgc gggagcctcg
      781 gtgctgcgcg tgaccgccca tgatgccgac gatgcggcgc gggtcgatgc ccgccaccga
      841 ctcggcgagc gcttcgctga tcgcgtgatc gtgcagggtg acggccaggt cggcgccgag
      901 ttcaccggga cgcagctgct gctgcgacca ggcgaagatg cgggcatcga gcgtggccgg
      961 gtagccgccg tccaggccgc ggtacagctc gtccgcggtg tagagggtcg ggcggtaggg
     1021 gtcgaacggc aggtcgggca ccggcggaaa caccagggca cccgccctga ccaggaatga
     1081 ggcatccgct ggtcgcaacg tgcagcccag aaagtgggcg ccggccacgt gggcgtcgcg
     1141 gagtgcggcc gagcgatggg tgaggtcgat cgactggacg acgaccccgg ccagcgaccc
     1201 cgtggcgccc aggtgtgcgt cgaactcgtc caggctttcc aggtcgatcc gcacggcatc
     1261 acgctagcgt gggcagccag gaggtccgcg atgccgcagc aggttcccca accggatccg
     1321 tcccggatga ccccgttcac cggcggtgcg atcgtggcgg ccgtgcgccc cggcagcgat
     1381 cccctggtcg cgctgaccgc cgccgatctg gcgcgggtca ccggtggaac cctgtacttc
     1441 gccttcgtcg acgagcagcg cttcgtcgag cgtgaactgc cggacggctc cgtgcgacac
     1501 gccgacctca acccggacgc cggtgacgac gcctggcgcc gccgcgaggc cggcatgacc
     1561 gccgacctgc gcaggttgct ggccgacgcg ggcgtgacct ggcagtttcg ctacctggcc
     1621 ggacgtccgg accgcgccct cacccatctg gccagggccg tggacgcagc ggtgatcgtg
     1681 gtcggccacc acgcacccga gcggggggcc agggttcgcg agttcctgga cggtgccctc
     1741 gccctcaagc tcgcccagca ccagcatcgt ccggtgttgg ccgtgcccct tcgcgtggtc
     1801 gactggaagg cgccgatgcc gtggggctga gcacggcgcg gggcgccacg acgctggtgg
     1861 tgttcgccgg cggcgccctc ggcacagcga ctcgggccgc tctggaggcg gccaatcccg
     1921 cgccccaggg agggtggccg tgggcgacgt tcctgatcaa cgtcagcggg gcgttcgtgc
     1981 tgggtctgct gctcgaactg ctcagccgcc gcggcccgga cgtaggggca cgccggttgc
     2041 tgcgcctggg cctgggcacc ggggtgatgg gcggctacac cacctacagc accttcgccc
     2101 tcgagaccgt ccggttgctc gaagggggag cgctgctgct cgggctcggc tacgcgctgg
     2161 gcagcgtgct gctcgggctg gcggccgcct gggccggcac gctgctcgcg atcaggatcg
     2221 cgccgtgatc gcgctgctgg tcgccctggc gggcgggttg ggggccgtcg cgcggttcca
     2281 ttccgacgca ttcatcgccc gcgtcaaccg gttgccgttg ccgctcggca cgctggtgat
     2341 caacaccgtc gggtcgctgt tgttggggct gttcagcggg tgggtggtct tccattccgg
     2401 cgcgccggcg tgggccggca tcgccggcac cgggttctgc ggcggtttca ccacgttcag
     2461 caccgcctgc gtcgaggggt ggcggctggt gcgtgcggga cgtccggtcg ctgcggtact
     2521 gcacgccggc gggggactgg tggcgggcct gctggcggcg tcgctcgggt tgtggctgat
     2581 gtcgctgtga tcgggttcgc tcatgccgga cgcgctgtgg tccgggctcg ctcgtcccgg
     2641 acgcgctgtg gtcccggggt tccgtgctcg ctgtggtccg ggctccggtg atatcggctc
     2701 ggtcccaagt ttgctgggcg ccgccttgta ccctgtccgg gtgcgtgatg tcgtcctgct
     2761 gggaagcacc ggttcgatcg gttctcagac ccttgaggtg atcgaggcac accctgagaa
     2821 gttccgggtc atcggattgt cggcgggggg ttcccagctc gacaccctga tcgcgcaggc
     2881 gcaggcccac cgtccggacg tggtcgccgt gccgactccc gctgccgccg ccgccctcga
     2941 gggacgcctc cccgccgata ccgcgatctg gaccggcccc gatgcgtccg ctcggctcgc
     3001 cgcactgccc tgcgacgtgg tggtcaacgc gatcaccggc gccgcgggac tcgaacccac
     3061 cctggccgcg ctcgccgccg gcaacgccct ggccctggcc aacaaggagt cgttggtgat
     3121 cggcggcgaa ctggtcaccg ccgcggccgc gccgggccag attctggccg tcgattccga
     3181 gcactcggcc ttcgcccagg cccttcgtgc cggacgctcc gatgaggtgc gcaggctgat
     3241 cctgaccgca tccggcggcc cgttccgcgg ccgcacccgt gacgagttga ccgacgtcac
     3301 tcccgagcag gcgctggccc acccgacctg gtccatgggc cgcatcatca ccatcaactc
     3361 cgccaccctg atcaacaagg gcctcgaact gttggaggct gcgctgctct acggtgtcga
     3421 cctggacgcg atcgaggtgg tcgtgcaccc gagttccgat gtgcattcga tggtggagtt
     3481 tcacgacggc tcgacgatcg cgcaatgttc accgcccgac atgaagctgc cgatcgccct
     3541 ggcgctcaac tggcccgagc ggctgccggg cgcgagccgt ccgttcgact ggtcgcggcc
     3601 gatcgactgg cacttcgaac cgctcgactc gcacgccttc ccctcggtcg agctggccag
     3661 acgctgcgga cgggcgggcg gcaccgcccc ggcggtgtac aacgcggcga acgagatctg
     3721 cgtggacgcc ttctgtgagc gtcggctcgg ctttctgggc atcgtcgaca ccgtcaccgc
     3781 cgtggtcgac gagcacctgg ccggtgccgc gaaccaggct cccatgagcg tcgattcggt
     3841 gctggacgct gacgcctggg cgcgtcggcg cgccgccgaa ctgatcgggg tggccggatg
     3901 aacggcctgg tcacagtcgt cttcgcggtg gtgttcttcg gctgcatcct gctgtcgatc
     3961 gccctgcacg agatcggcca catgctgccc gcgaaacgct tcggggtgcg ggttccgcag
     4021 tacttcgtcg gcttcgggcc gactatgtgg tcgacggtcc ggggtgagac cgagtacggc
     4081 atcaagtgga ttccgctcgg cggcttcgtc cggctgctcg gcatgtatcc gccgggtcgc
     4141 acgcgcaccg gccagggcaa ccggctgacc cggttcgccg acggtgcccg cgcgtacgag
     4201 tgggaagaga tcacccccga cgacgtcgcc aacgagcggt tgttctacca gaagaagacc
     4261 tggcagaagc tggtcgtgat ggccggtggg ccgatgatga acgtgctgat cgcgttcgtt
     4321 ctgttctggg cgatcctggg cctgcacggc agttatcagg cgcagccggt cgtcgcgcag
     4381 atccagcagt gcatcgtgcg cgagaatcag cccgccgaca ccccgtgccc gacgcccgac
     4441 accccagcgg ccaagtcggg catcaaggca ggcgacaccg tcgtcgagtt caacggccac
     4501 cagatcaccg actacgccca actgcagggg ttgatccggg ccaacctcga cggtgaggcc
     4561 cggatggtgg tcgaacgtga cgggcagcgg gtctcgctga cccccgtcca caccgtgatc
     4621 agtggcgtgc aagacaacct cgaccccagc aagcgcatcg cggccggttg gttcggcatc
     4681 accccgacca ccgagttggt caagggcggc ccggtcgaaa ccgtgcagca gatgtggacc
     4741 atgaccaccc agagcgtggt ggccatcacc cagttcccgg tcaaggtcta caacgtggtc
     4801 gccgacctgg tgcagggtaa gccacgcgac atctacggtc cgctgagcat cgtgggcgcc
     4861 agccgggcag ccggcgaggt ggtggccagc gacgcgatca
//
LOCUS       DAOSUG010000203         4888 bp    DNA     linear   ENV 10-JUN-2024
DEFINITION  MAG TPA_asm: Micropruina sp. isolate AMR_MDS_2195
            NODE_7590_length_4888_cov_3.690461, whole genome shotgun sequence.
ACCESSION   DAOSUG010000203 DAOSUG010000000
VERSION     DAOSUG010000203.1
DBLINK      BioProject: PRJNA925477
            BioSample: SAMN32864262
            Sequence Read Archive: SRR4236673
KEYWORDS    WGS; ENV; Metagenome Assembled Genome; MAG; Third Party Data; TPA;
            TPA:assembly.
SOURCE      Micropruina sp. (wastewater metagenome)
  ORGANISM  Micropruina sp.
            Bacteria; Bacillati; Actinomycetota; Actinomycetes;
            Propionibacteriales; Nocardioidaceae; Micropruina.
REFERENCE   1  (bases 1 to 4888)
  AUTHORS   Abdulkadir,N., Saraiva,J.P., Zhang,J., Stolte,S., Gillor,O.,
            Harms,H. and Rocha,U.
  TITLE     Genome-centric analyses of 165 metagenomes show that mobile genetic
            elements are crucial for the transmission of antimicrobial
            resistance genes to pathogens in activated sludge and wastewater
  JOURNAL   Microbiol Spectr 12 (3), e0291823 (2024)
   PUBMED   38289113
REFERENCE   2  (bases 1 to 4888)
  AUTHORS   Abdulkadir,N.
  TITLE     Direct Submission
  JOURNAL   Submitted (24-MAR-2023) Environmental Microbiology, Helmholtz
            Center for Environmental Research -UFZ, Permoserstr 15, Leipzig
            04318, Germany
COMMENT     The annotation was added by the NCBI Prokaryotic Genome Annotation
            Pipeline (PGAP). Information about PGAP can be found here:
            https://www.ncbi.nlm.nih.gov/genome/annotation_prok/
            
            ##Genome-Assembly-Data-START##
            Assembly Method        :: SPAdes v. 3.11.1
            Genome Representation  :: Full
            Expected Final Version :: Yes
            Genome Coverage        :: Not Applicable
            Sequencing Technology  :: Illumina MiSeq
            ##Genome-Assembly-Data-END##
            
            ##Genome-Annotation-Data-START##
            Annotation Provider               :: NCBI
            Annotation Date                   :: 05/04/2023 04:57:10
            Annotation Pipeline               :: NCBI Prokaryotic Genome
                                                 Annotation Pipeline (PGAP)
            Annotation Method                 :: Best-placed reference protein
                                                 set; GeneMarkS-2+
            Annotation Software revision      :: 6.5
            Features Annotated                :: Gene; CDS; rRNA; tRNA; ncRNA
            Genes (total)                     :: 2,776
            CDSs (total)                      :: 2,750
            Genes (coding)                    :: 2,714
            CDSs (with protein)               :: 2,714
            Genes (RNA)                       :: 26
            tRNAs                             :: 24
            ncRNAs                            :: 2
            Pseudo Genes (total)              :: 36
            CDSs (without protein)            :: 36
            Pseudo Genes (ambiguous residues) :: 0 of 36
            Pseudo Genes (frameshifted)       :: 2 of 36
            Pseudo Genes (incomplete)         :: 32 of 36
            Pseudo Genes (internal stop)      :: 2 of 36
            ##Genome-Annotation-Data-END##
FEATURES             Location/Qualifiers
     source          1..4888
                     /organism="Micropruina sp."
                     /mol_type="genomic DNA"
                     /submitter_seqid="NODE_7590_length_4888_cov_3.690461"
                     /isolate="AMR_MDS_2195"
                     /isolation_source="Activated sludge of municipal
                     wastewater treatment plants"
                     /db_xref="taxon:2737536"
                     /environmental_sample
                     /geo_loc_name="Canada"
                     /lat_lon="49.25 N 123.1 W"
                     /collection_date="2015-06-16"
                     /metagenome_source="wastewater metagenome"
                     /note="metagenomic"
     gene            <1..277
                     /locus_tag="PLF56_07680"
     CDS             <1..277
                     /locus_tag="PLF56_07680"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_015070871.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=2
                     /transl_table=11
                     /product="transcription termination/antitermination
                     protein NusA"
                     /protein_id="HOQ53479.1"
                     /translation="GQRVRAVMHELNEEKIDIIDYSDDPAVFVKEALSPAKVSQVIVT
                     DATARAARVIVPDYQLSLAIGREGQNARLAARLTGWRIDIRPDTQAE"
     gene            complement(507..1007)
                     /locus_tag="PLF56_07685"
     CDS             complement(507..1007)
                     /locus_tag="PLF56_07685"
                     /inference="COORDINATES: ab initio
                     prediction:GeneMarkS-2+"
                     /note="Derived by automated computational analysis using
                     gene prediction method: GeneMarkS-2+."
                     /codon_start=1
                     /transl_table=11
                     /product="hypothetical protein"
                     /protein_id="HOQ53480.1"
                     /translation="MNLVRGARLPSLCMCLAIALSGCTVTPLPAPDVPATSPVPTQIH
                     SGLELDRSLRAGQEQGAVTLPQLGRLLAATQTGGVTAIHVQLEHVTGPVVAAFICEGD
                     TAGPKVTLRRGGKTLLRIRTDGCDASNLYSGQSESIGEGGPAELMIAAPEGLRYALVL
                     EEVAES"
     gene            complement(1018..1341)
                     /locus_tag="PLF56_07690"
     CDS             complement(1018..1341)
                     /locus_tag="PLF56_07690"
                     /inference="COORDINATES: ab initio
                     prediction:GeneMarkS-2+"
                     /note="Derived by automated computational analysis using
                     gene prediction method: GeneMarkS-2+."
                     /codon_start=1
                     /transl_table=11
                     /product="hypothetical protein"
                     /protein_id="HOQ53481.1"
                     /translation="MTLSISSGISHTIGGTVTGTVEVEAGVLFAKASASLVTVGGSVT
                     TTSNSGGSWTVTKKVGWLEVGTRSGYSFNWTEFQHKAPGTKVIQGKGTGKAAAKDAAA
                     YYKHS"
     gene            complement(2225..2779)
                     /locus_tag="PLF56_07695"
     CDS             complement(2225..2779)
                     /locus_tag="PLF56_07695"
                     /inference="COORDINATES: ab initio
                     prediction:GeneMarkS-2+"
                     /note="Derived by automated computational analysis using
                     gene prediction method: GeneMarkS-2+."
                     /codon_start=1
                     /transl_table=11
                     /product="Clp protease N-terminal domain-containing
                     protein"
                     /protein_id="HOQ53482.1"
                     /translation="MDSKQKTGLAIGAAVIAAGAVFGVSYALGSSSDGSDTAQSAPTA
                     QSTEGGRPGGGGGMGNMSNLASALATKLGVDEAKVTEAIQSAMSANRPSGAPSDGAQP
                     GGQPTDGGQPPTDGGSGSQPSGRPSMGGDRSGMDATLAKAIAEKLGIDEAKVTKALAE
                     VRAEQQASRSTTTASPQPSASASA"
     gene            3273..3875
                     /locus_tag="PLF56_07700"
     CDS             3273..3875
                     /locus_tag="PLF56_07700"
                     /inference="COORDINATES: ab initio
                     prediction:GeneMarkS-2+"
                     /note="Derived by automated computational analysis using
                     gene prediction method: GeneMarkS-2+."
                     /codon_start=1
                     /transl_table=11
                     /product="hypothetical protein"
                     /protein_id="HOQ53483.1"
                     /translation="MLSNQPTPRRPAFRDPAMLELMGEGSDPIAQLHAAHETARVLLH
                     TGRAGDDPSVTARLMEIVDEIGISTLADLWSSRPPRSLPGALWRLYYLREWMRTDADG
                     VARAYSAGVRFTEPNHVVAGIDPPTPADVRRVADDILRGAFDGDFAVALERAAALCAV
                     TSAGSSEIAGQEALMRATKLQQLSADLTAAAALWRAGRLS"
     gene            4582..4854
                     /locus_tag="PLF56_07705"
     CDS             4582..4854
                     /locus_tag="PLF56_07705"
                     /inference="COORDINATES: protein motif:HMM:NF016204.2"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="YlxR family protein"
                     /protein_id="HOQ53484.1"
                     /translation="MTSPQRMCAGCRERADKATLTRLVWNGADSVMVDDRQRLPGRGV
                     YVHSGCVERALKVRAIARGLKRSLDGVALREALAALSARGADASSD"
BASE COUNT          718 a         1633 c         1681 g          856 t
ORIGIN      
        1 ggggcagcgg gtgcgcgcgg tgatgcacga gctgaacgaa gagaagatcg acatcatcga
       61 ctactccgac gatccggcgg tgttcgtcaa ggaggccctg tcgccggcca aggtgtcgca
      121 ggtgatcgtg acggacgcga ccgcccgggc cgcccgggtg atcgtgcccg actaccagtt
      181 gtcgctggcg atcggacgcg agggccagaa cgcccgtctc gccgcccggc tgaccggttg
      241 gcgcatcgac atccggcccg acacgcaggc ggagtagccg caccggcgcc gatcaactgt
      301 gccgacggcc gcaaccgagt cgcgaaccgc tcgaccggcg gtgccgggtc gctgcacgtg
      361 ggcccgtcgg aggggctcag gggctggatc gccgaaacct cggcgacggc ggctcggccg
      421 gcggggggcc agggggggtc tgcagcagtt gagcgccgac ttgagcgtct gtgcccggtt
      481 atggctcgcc gtaacgctca cgtgagtcag ctctcggcaa cctcctccag cacgagggcg
      541 tagcgcaggc cctccggtgc cgcaatcatc aactctgcag ggccaccttc tccgattgat
      601 tcactctgcc cgctatagag attcgatgcg tcgcacccgt cggtcctgat gcgcaacagg
      661 gtcttgccac ctcggcgaag agtcaccttt gggccggccg tgtcgccttc gcagatgaag
      721 gccgcgacga ccgggccggt gacgtgctcg agttgcacgt gaatcgccgt gacgccgccg
      781 gtttgggtgg cagccagcag tcgccccaac tgcggaagag taaccgctcc ttgttcttgt
      841 ccggctcgca aggatcgatc gagttccagg ccgctatgga tctgggtggg aactggtgag
      901 gtggctggca catcgggagc cggcaaaggc gtgacggtgc aacctgatag ggcgatcgcc
      961 aggcacatgc agagactcgg caggcgcgcg ccacgaacga ggttcatcgg ggcgaagtca
     1021 actgtgcttg tagtaggccg ccgcatcttt ggccgccgcc ttccctgtgc ccttaccttg
     1081 aatcaccttg gtaccaggcg ccttatgctg aaattcggtc cagttgaacg agtacccgct
     1141 cctcgtaccc acttcgagcc agcccacttt cttggtgact gtccacgacc cgccactgtt
     1201 gctcgtggtg gttactgagc cacctacggt cacaaggctt gcgctggcct ttgcgaagag
     1261 cacgccagct tccacctcca ccgtgcccgt gacggtcccg ccaattgtgt gcgatattcc
     1321 gctcgagatg ctcagagtca cgccacggtc accgtgagtg cgtgaccctc ttgaagccat
     1381 gtagatcttc ttcttgttgc agatgctgta ctcacttgcc gtgcgcgtac gtgttgggga
     1441 caacacgttg gcctgcggat ggtggaccca cgggcgaccc ttggcagatc acactttggg
     1501 gcgtggctgc atatcggcct gcatccgcgg ccgcaacggg cgtgcagact atcaatcccg
     1561 caatcgcacc ggcggcggca agggcaagtc tttgttgttt caatcggcca gtgaactctc
     1621 gtcatggggt gtgagttgtc gagagtgtaa cgctcagcag cctctcgagt gcgagcgatt
     1681 ccagtcggcg cgccacgttg gctattgccg gagtccaggt agcgagcggc agtgcaacct
     1741 ctgctgagtg gtggtctcgc caaacgtttt gcatagggcg agactcggtg cctcatgtgc
     1801 ttccgcccag cgtggttccg cttgaccttc gcccacacca tgttcttgac actttagggc
     1861 cttgggcgcc tctggaaggg gggtcggtgt caggaggtcg gtttggggtc gcgacgcgct
     1921 cgaccggcgg tgccggattc gtcacacgca ttcgtccgca cacgacccgg cacccactcg
     1981 ctaggtgggt gccgggtcgc ttcacgtggt gcccgccgga ggggatcagg ggctcgatcg
     2041 ccgaaacctc ggcgatggcg gctcggccgg cggggggtcg aggggtcggg cggacgcgct
     2101 ggcgcccggc cgggttcgcg ttgactaccg gagctgacgg cgagggcgcg tcgctcgggg
     2161 ggacgcgttg gttccaggcg attgcctgag ccggcggggc gtggtgctca gcccggatcc
     2221 cggctcaggc ggacgcgctg gcgctcggtt gcggcgaggc ggtcgtggtg gagcgactgg
     2281 cctgctgttc cgcccttacc tctgcgagtg ccttggtcac cttggcctcg tcgatgccga
     2341 gcttctcagc gatcgccttg gccaaggtgg cgtccatgcc ggagcggtct ccacccatgg
     2401 acggacgtcc gctcggctgg ctgccgctgc cgccatcggt ggggggctga ccgccgtcgg
     2461 tcggctggcc gcccggctgc gctccgtcgc tgggtgcacc gctgggccgg ttggcgctca
     2521 tcgccgactg gatcgcctcg gtgaccttgg cctcgtccac accgagcttc gtggccagcg
     2581 ctgaggcgag attgctcatg ttgcccatgc cgccgccacc gcccggccgt ccgccctcgg
     2641 tgctctgggc ggtgggggca gactgagcgg tgtcggagcc atcactgctg ctgcccagcg
     2701 cgtacgacac gccgaacacg gcgcccgctg cgatcacggc ggcgccgatc gccagcccgg
     2761 tcttctgttt gctgtccatt gtggtgttga ctctcctggt catcagcgtt cgtgtagtga
     2821 tcaccagtga gccggaccgg gctggcagcc cgctgatgca aacctgtgtg gggcctgtga
     2881 gcccccgcgc aaacagggcg tcaggtactc aggggaccac gatggctgcg acgctccgtg
     2941 gagcggctcg cggtcgatgt gcgaaagcgc gttgggcgtg gccggctcag ccaggtcggc
     3001 ccaagtggag agtgcgcagt cgcagtagac ggtgatcggt gggtccgggt tcgcaaagtc
     3061 cggggttggc ccggcaatcg accggcacgt cggcctggtt gcgcgtctga ttgcgagcgg
     3121 gtgggttttg gcacagcagt cggtgccgaa acccacccgc tccgcgcagt gcgtcggatc
     3181 acccggtgcg tccctcgaga ttgtgcccag accggggccg atcgcacagc gacgaacgag
     3241 gccctcggcg aatccggggt taggctgaac cggtgctgag caaccaaccg actccccgcc
     3301 gaccggcgtt tcgcgacccc gcgatgctcg agttgatggg cgagggttcc gacccgatcg
     3361 cccagttgca cgccgcccac gaaacggcgc gggtgctgct gcacaccgga cgcgccggcg
     3421 acgacccgtc cgtcaccgcg cgactgatgg agatcgtgga cgagatcggc atctcgacgc
     3481 tggccgacct gtggtcatcg cggccgccgc gcagcctacc gggcgcactg tggcggctgt
     3541 actacctgcg cgagtggatg cgtaccgacg cggacggggt cgcccgggcc tactcagccg
     3601 gcgtgcggtt caccgagccg aatcacgtcg tggccggcat cgatccgccc acgcccgccg
     3661 acgtgcgacg ggtggccgac gacattctgc gcggcgcctt cgacggggac ttcgccgtcg
     3721 cactggagcg cgcggcggcc ttgtgcgccg tgacgtccgc cggttcgtcg gagatcgccg
     3781 gacaggaggc gctgatgcgc gccacgaaac tccagcaact ctccgccgac ctgaccgccg
     3841 ccgccgcgct ctggcgcgcc ggacgactca gctgagctgc gtccgcccac ccggcttccc
     3901 tcggcccgca ccaacgcact gggtacgggc gcaccgggta cgggcgccgc gcacaccggc
     3961 cgcgcccgca gcagcaccag ttgacgcacg aatcgccgtg cgagagcgct tccggtttgg
     4021 gtcggcgcac gtggcggcct atattcgttc acggcgccgg actgcggtag ccccgggctc
     4081 caacacttgc cgcttcgagc ggccccgcgc cgagaggcgc tcccagtccg gcgccactta
     4141 ccttccgctg acaccgtgcg cgaatgacca cgtgggtcga ctcgagaaag cccggaccga
     4201 tgacgaccac cagcgcacgc gaaaacgact gaacccggcc gtcgggggga atgacggccg
     4261 ggtcgggaag aggaatcagt cgatcttgtg ctgccaggtg accacctccg ctcgctcggt
     4321 gtactgctcg atgtctctac gaaactcccc gagcctgggt gctgtctggt ccgccactgt
     4381 gtgctcgctg tgaatgccga ccgctcatca tggatcaacc gatctgcgcc tactgcgcgg
     4441 cctcgtccgg gcaccctggc tgcgtcggcc acgctgccca cacgtccact atgccttcgc
     4501 gcgagaccgg catgcccggc cacccgtctg gtgccgctcg cgacggcgcc caactgtgga
     4561 tccaggctaa tctgagcggc gatgacttcg ccccaacgca tgtgcgccgg ctgccgcgag
     4621 cgggccgaca aggccacact gacccggctg gtctggaacg gtgcagactc cgtgatggtg
     4681 gacgatcggc aacggctacc cggccgaggc gtctacgtgc actcgggctg cgtcgagcgt
     4741 gcgctgaagg tgcgggccat cgcccgtggc ctgaaacgca gcctggacgg ggtcgcgctc
     4801 cgtgaggcac tggccgccct gtctgctcgt ggggccgacg cgtcatccga ctgagcgcgg
     4861 tcgtgatcag ggcagggtct cgtgatcc
//
LOCUS       DAOSUG010000204         4848 bp    DNA     linear   ENV 10-JUN-2024
DEFINITION  MAG TPA_asm: Micropruina sp. isolate AMR_MDS_2195
            NODE_7653_length_4848_cov_3.677655, whole genome shotgun sequence.
ACCESSION   DAOSUG010000204 DAOSUG010000000
VERSION     DAOSUG010000204.1
DBLINK      BioProject: PRJNA925477
            BioSample: SAMN32864262
            Sequence Read Archive: SRR4236673
KEYWORDS    WGS; ENV; Metagenome Assembled Genome; MAG; Third Party Data; TPA;
            TPA:assembly.
SOURCE      Micropruina sp. (wastewater metagenome)
  ORGANISM  Micropruina sp.
            Bacteria; Bacillati; Actinomycetota; Actinomycetes;
            Propionibacteriales; Nocardioidaceae; Micropruina.
REFERENCE   1  (bases 1 to 4848)
  AUTHORS   Abdulkadir,N., Saraiva,J.P., Zhang,J., Stolte,S., Gillor,O.,
            Harms,H. and Rocha,U.
  TITLE     Genome-centric analyses of 165 metagenomes show that mobile genetic
            elements are crucial for the transmission of antimicrobial
            resistance genes to pathogens in activated sludge and wastewater
  JOURNAL   Microbiol Spectr 12 (3), e0291823 (2024)
   PUBMED   38289113
REFERENCE   2  (bases 1 to 4848)
  AUTHORS   Abdulkadir,N.
  TITLE     Direct Submission
  JOURNAL   Submitted (24-MAR-2023) Environmental Microbiology, Helmholtz
            Center for Environmental Research -UFZ, Permoserstr 15, Leipzig
            04318, Germany
COMMENT     The annotation was added by the NCBI Prokaryotic Genome Annotation
            Pipeline (PGAP). Information about PGAP can be found here:
            https://www.ncbi.nlm.nih.gov/genome/annotation_prok/
            
            ##Genome-Assembly-Data-START##
            Assembly Method        :: SPAdes v. 3.11.1
            Genome Representation  :: Full
            Expected Final Version :: Yes
            Genome Coverage        :: Not Applicable
            Sequencing Technology  :: Illumina MiSeq
            ##Genome-Assembly-Data-END##
            
            ##Genome-Annotation-Data-START##
            Annotation Provider               :: NCBI
            Annotation Date                   :: 05/04/2023 04:57:10
            Annotation Pipeline               :: NCBI Prokaryotic Genome
                                                 Annotation Pipeline (PGAP)
            Annotation Method                 :: Best-placed reference protein
                                                 set; GeneMarkS-2+
            Annotation Software revision      :: 6.5
            Features Annotated                :: Gene; CDS; rRNA; tRNA; ncRNA
            Genes (total)                     :: 2,776
            CDSs (total)                      :: 2,750
            Genes (coding)                    :: 2,714
            CDSs (with protein)               :: 2,714
            Genes (RNA)                       :: 26
            tRNAs                             :: 24
            ncRNAs                            :: 2
            Pseudo Genes (total)              :: 36
            CDSs (without protein)            :: 36
            Pseudo Genes (ambiguous residues) :: 0 of 36
            Pseudo Genes (frameshifted)       :: 2 of 36
            Pseudo Genes (incomplete)         :: 32 of 36
            Pseudo Genes (internal stop)      :: 2 of 36
            ##Genome-Annotation-Data-END##
FEATURES             Location/Qualifiers
     source          1..4848
                     /organism="Micropruina sp."
                     /mol_type="genomic DNA"
                     /submitter_seqid="NODE_7653_length_4848_cov_3.677655"
                     /isolate="AMR_MDS_2195"
                     /isolation_source="Activated sludge of municipal
                     wastewater treatment plants"
                     /db_xref="taxon:2737536"
                     /environmental_sample
                     /geo_loc_name="Canada"
                     /lat_lon="49.25 N 123.1 W"
                     /collection_date="2015-06-16"
                     /metagenome_source="wastewater metagenome"
                     /note="metagenomic"
     gene            complement(<1..918)
                     /locus_tag="PLF56_07710"
     CDS             complement(<1..918)
                     /locus_tag="PLF56_07710"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_002548672.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="ATP phosphoribosyltransferase regulatory
                     subunit"
                     /protein_id="HOQ53485.1"
                     /translation="MSRPKPLSGFPEFLPAARIVEQRVIAALADTFELHGFGSIETRA
                     VEPMAQLARKGEIDKEVYVVRRLHAEGTADADLGLHFDLTVPFARYVLENSGHLSFPF
                     RRYQIQKVWRGERPQEGRYREFLQADIDVVGQDTLAAHHDVEVAVVILEALERLHTEF
                     GVPPVLMRVNNRKLAEGFYRGLGIDDHLAVLQRVDKLDKIGPAAVADLLTGELGLSDQ
                     VAAKCLELATISAADESFVERVRALGVENELLDEGLAQLADLVAAAAAAVPGRLVADL
                     KIARGLDYYTGTVYETEMTGFEHLGSICSG"
     gene            complement(915..1613)
                     /locus_tag="PLF56_07715"
     CDS             complement(915..1613)
                     /locus_tag="PLF56_07715"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_013863714.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_function: GO:0016787 - hydrolase activity [Evidence
                     IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="MBL fold metallo-hydrolase"
                     /protein_id="HOQ53486.1"
                     /translation="MFVASFPSGPWQANCYLAAAAAASPCVIVDPGVGAAPAVRQLLA
                     AHQLTPAGVLLTHGHIDHIASAAELADEWRVPTWIHAADRELLTDPVAGLGPSAGPLL
                     ASVIGDDPLVEPHDLRLFDSGVDVAGLSFEVIHAPGHRPGCVMLRTMLSGNDRADRLV
                     FSGDVLFAGSIGRTDLPGGDHAAMLDTLRGPVLGLTDTSAILPGHGRQTLMAAERATN
                     PYLQADYLGGALAR"
     gene            1685..2926
                     /locus_tag="PLF56_07720"
     CDS             1685..2926
                     /locus_tag="PLF56_07720"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_008599680.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_function: GO:0003676 - nucleic acid binding [Evidence
                     IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="DUF349 domain-containing protein"
                     /protein_id="HOQ53487.1"
                     /translation="MTSSSPADFGRVDPDGTVYVRTSEGERSVGQIPDVPEAEALAFY
                     TRRFEALALEVGLLERRIASGALSPEEARHSINTVTGNVAGANAVGDLDGLHERLKAL
                     APVLAQASEARKAERAKQAEATKATKEAMVAEAEQLAQGNDWRGGVNRFRSLLDEWKA
                     LPRIDRATDDDLWHRFSTARTTYTRRRKTQFAEQAEKRDNARQLKEQIIAEARPLAES
                     TDWGPTSGAFRDLMARWKAAGAAPREVDDALWAEFRGLQDQFFNARTATQSQQDEEFK
                     ANLDAKEALLAKAEEEVVPVTDLAASRSAFRTFVEQFNALGKVPRESIRGLDNRVKAL
                     ENAVRQAEDAEWKRTDPEARARAEETVAMLSSEIAKLTEKITKAETRGDQKAADKAKD
                     SIATYQSWLDQARETLADFSR"
     gene            complement(3261..3452)
                     /locus_tag="PLF56_07725"
     CDS             complement(3261..3452)
                     /locus_tag="PLF56_07725"
                     /inference="COORDINATES: ab initio
                     prediction:GeneMarkS-2+"
                     /note="Derived by automated computational analysis using
                     gene prediction method: GeneMarkS-2+."
                     /codon_start=1
                     /transl_table=11
                     /product="hypothetical protein"
                     /protein_id="HOQ53488.1"
                     /translation="MELTEVAAADFGVRLTFHRLPSGPPRTDTIERVRGYMLECGATT
                     ASEVAAMWQQELDEGVLSQ"
     gene            complement(3605..>4848)
                     /locus_tag="PLF56_07730"
     CDS             complement(3605..>4848)
                     /locus_tag="PLF56_07730"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_013161061.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=3
                     /transl_table=11
                     /product="TGS domain-containing protein"
                     /protein_id="HOQ53489.1"
                     /translation="PDSKPVEFQIRTHEMHRQAEYGVAAHWKYKEGKATGAGNDLAWV
                     RQITEWQKETEDPGEFLDTLRFEINTDEVFVFTPKGDVISLPANSTPVDFAYAVHTEV
                     GHRTIGSRVNQRLVPLEYQLNNGDQVEVLTSKSPNAGPSRDWLNFVASPRARTKIRQY
                     FMRERREESIEQGKEALAKQLRRTGLPVHRLLSAEHVAALARHFRVADVNALYAAIGE
                     GTVSAQNVVERLIAAEGGAEEAAEASVEDHATITTSPRRRPSRRTNDLGVVVQGHSDM
                     WVKLAKCCMPVPGDDIIGFITREDGVSVHRADCTNADNLRGKPERLVEVSWATPSADS
                     SFLVAVHVEGLDRAGLLSDVSRVMTEHHSNILSMSANTSKDRVFTMRLQFETPDPTHL
                     ASLTNALKRIPGVYEANRVKS"
BASE COUNT          811 a         1693 c         1585 g          759 t
ORIGIN      
        1 cccagagcag atcgagccga ggtgctcgaa gcccgtcatc tcggtttcgt agacggtgcc
       61 ggtgtagtag tcgagcccgc gggcgatctt gaggtcggcc accagtcgtc cgggcaccgc
      121 ggcggccgcc gcggccacca ggtcggccaa ctgggccagc ccctcgtcca gcagttcgtt
      181 ctcgacgccc aacgcccgaa cccgctccac gaacgactcg tcggcagccg agatcgtcgc
      241 gagttcgaga catttggccg ccacctggtc gctcaacccg agttcaccgg tgagcaggtc
      301 ggcgaccgcc gccggcccga tcttgtcgag cttgtcgacc cgctgcagca cggccaggtg
      361 atcgtcgatg cccaggccgc ggtagaagcc ctcggccagc ttgcggttgt tcacccgcat
      421 cagcaccggc ggcaccccga actcggtgtg cagccgctcc aacgcctcga gaatcaccac
      481 cgccacctcg acgtcgtggt gcgcggccag cgtgtcctgg cccaccacgt cgatgtcggc
      541 ctgcaggaac tcgcgatacc ggccctcttg cggacgctcg ccacgccaca ccttctgaat
      601 ctggtagcgg cggaacggaa agctgaggtg ccccgagttc tcgagcacgt agcgcgcgaa
      661 cggaaccgtg aggtcgaagt gaaggcccag atcggcatcg gcggtgccct cggcgtgcag
      721 ccggcgcacc acgtagacct ccttgtcgat ctcgcccttg cgcgccagct gtgccatcgg
      781 ctcgaccgca cgcgtctcga tcgagccgaa gccgtgcagc tcgaaggtgt cggccagggc
      841 cgcgatcacc cgctgttcga cgatgcgtgc ggcgggcagg aactcgggaa aaccactcag
      901 cggtttggga cggctcatcg ggcgagtgca ccacctagat agtcggcttg cagatacgga
      961 ttcgtggcgc gttccgcggc catcagggtc tgccgaccgt ggcccggcag aatcgcggac
     1021 gtgtcggtga ggcccagtac ggggccgcgc agggtgtcga gcatcgccgc gtgatcgcca
     1081 ccgggcaggt cggttcgtcc gatcgatccg gcgaacagca cgtcgcccga gaacaccagc
     1141 cggtcggccc ggtcgttgcc gctcagcatc gtgcgcagca tcacgcagcc cggacggtgc
     1201 cccggcgcgt gaatgacctc gaaactcaac ccggcgacgt ccacaccgga atcgaaaagg
     1261 cgcaggtcgt gcggctcgac cagtggatca tcgccgatca ctgaggcgag taacggcccg
     1321 gcggacgggc cgagtcccgc gacgggatcg gtcagcaact cgcgatcggc ggcatggatc
     1381 cacgtcggca cccgccattc gtcggccagt tcggcagcgg aggcgatgtg atcgatgtgc
     1441 ccgtgcgtca gcagcacccc ggccggagtc agctggtgcg cggccagaag ctgtcggacg
     1501 gcgggcgcgg cacccacccc cggatcgacg atgacgcacg gtgatgccgc cgctgcagcg
     1561 gccagataac agttcgcctg ccacggaccg ctgggaaaac tggcgacgaa cacgggcgcc
     1621 agcctatcgg gcgtcggtgt gggcctggtc cacaaagatc ggaccatgag gcaagatggt
     1681 ccccatgact tcatccagcc cggctgattt cggccgcgtt gaccctgacg gcaccgtgta
     1741 cgtccgcacc tctgagggcg aacgcagcgt cggccagatc cccgatgttc cagaagccga
     1801 ggcgctggcc ttctacacca gacgcttcga ggcgctcgcc ctcgaagtcg gtctgctcga
     1861 acggcgcatc gcctcggggg ccctgtcgcc cgaagaggcg cggcacagca tcaacaccgt
     1921 gaccggcaat gtcgccggcg ccaatgcggt cggtgatctg gacgggctcc acgagcgact
     1981 caaggccctg gcaccggtgc tggcgcaggc gtccgaggcg cgcaaggccg agcgggccaa
     2041 gcaggctgag gccaccaagg cgaccaagga ggcaatggtc gctgaggccg agcaactggc
     2101 gcagggcaac gactggcgtg gcggcgtcaa ccgcttccgc tccctgcttg atgagtggaa
     2161 ggcactcccc cgcatcgacc gtgccaccga tgacgacctg tggcaccggt tctcgaccgc
     2221 gcgcaccacc tacacccgcc gccgcaagac gcagttcgcc gagcaggcgg agaagcgcga
     2281 caacgctcgt cagctcaagg agcagatcat cgccgaggca cgtccgctgg ccgagtcgac
     2341 cgactggggc cccacctcgg gcgcgtttcg tgacctgatg gcgcgctgga aggcggccgg
     2401 tgccgctccc cgcgaggtgg acgatgccct ctgggccgag ttccgcggcc tgcaggacca
     2461 gttcttcaac gcccgcaccg cgacccagtc gcagcaggac gaagagttca aggccaacct
     2521 ggacgccaag gaggcgctgc tggccaaggc cgaggaggag gtcgtgccgg tcaccgacct
     2581 ggctgcgtcg cgttcggcgt ttcgtacctt cgtcgagcag ttcaacgcct tgggaaaggt
     2641 gccgcgcgag tccatccgcg gactggacaa ccgggtgaag gcgctcgaga acgccgttcg
     2701 ccaagccgag gacgccgagt ggaagcgcac cgaccccgag gcccgcgccc gtgccgaaga
     2761 aacggtggcg atgctcagca gcgagatcgc caagctgacc gagaagatca ccaaggccga
     2821 gaccagaggc gatcagaagg ccgctgacaa ggccaaggac tcgatcgcga cgtatcagtc
     2881 ctggctcgat caggcccgcg agaccctcgc cgacttctcc cgctaggccg cctgtcgaga
     2941 cccgtaccca caccgtcatc gcagcgaaag ccgggatctc taccgaccgc tgtgatgtcg
     3001 gccacacacc ctcgcgggac cgtcacctgc acagccggat cccggcgttc gactcacagc
     3061 cgggatgacg accactcgac gcccgtgact cccccaccgg ccgagccgaa ccctgaccgg
     3121 gacgccccgc tgctttaggt ctgcatcgag caccgtcgag acccccatcc tcagcgcgtc
     3181 cgccgggatc ccggcgttcg ccggcatgac gaccctgcgc gccggtgact gtcgtcagtc
     3241 gagaagtcgt gactcgtcac ctactgagaa aggacgcctt catcgagttc ttgctgccac
     3301 atcgccgcga cctcggacgc cgttgttgcg ccgcattcga gcatgtaccc ccgcactctt
     3361 tcaatcgtgt ctgtacgcgg cggcccagac ggtaggcggt ggaaggtcaa cctgacgccg
     3421 aaatcggcgg ccgccacctc ggtcagttcc accccgctca cctgggctcc gcggcgaggt
     3481 ctacccaagt cgagagccgc gaagctttcg cgcaactggt cacgaaaccg cagcgcctcc
     3541 ttcttcagcc gcttgcgagc tacacgcgcg atcgggctcg cacctgcgcg gtgcgagagc
     3601 cacctcagct cttgacccgg ttggcctcat acacacccgg aatgcgcttg agcgcgttgg
     3661 tcagggacgc cagatgggtg ggatcgggcg tctcgaactg cagccgcatg gtgaacaccc
     3721 ggtccttgga cgtgttggcc gacatggaca ggatgttgga gtggtgctcg gtcatcaccc
     3781 ggctgacgtc cgacagcagg cccgcccggt ccagtccctc cacgtgcacc gccaccaaga
     3841 agctcgaatc ggcggacggg gtggcccagc tcacctcgac cagtcgttcc ggtttgccgc
     3901 gcaggttgtc ggcgttggtg cagtcggctc ggtgcaccga caccccgtcc tcgcgcgtga
     3961 tgaagccgat gatgtcgtcg ccgggcaccg gcatgcagca cttggccagc ttcacccaca
     4021 tgtcggagtg cccctgcacc accacgccca ggtcgttggt gcgccgggac ggccggcgac
     4081 gcggcgaggt ggtgatcgtc gcgtgatcct cgacgctggc ctcggcggcc tcttcggcgc
     4141 caccctcggc ggcgatcagt cgctccacca cgttctgggc gctgaccgtt ccctcgccga
     4201 tcgcggcgta gagggcgttg acgtccgcga cccgaaagtg ccgggccagg gcggcgacgt
     4261 gctcggcgga cagcagccgg tgcaccggca ggccggtgcg gcgcaactgc ttggccagcg
     4321 cctccttgcc ctgttcgatc gactcttcgc ggcgctcacg catgaagtac tggcggatct
     4381 tggtgcgcgc cctgggactg gccacgaagt tgagccagtc acggctgggc cccgcgttcg
     4441 gcgacttgga cgtcagcacc tcgacctggt cgccgttgtt gagttggtac tccagcggca
     4501 ccaggcgctg gttcacccgg gagccgatcg tgcgatgccc cacctcggtg tgcaccgcgt
     4561 aggcgaagtc gacgggcgtg gagttggccg gcagtgagat cacgtcgccc ttgggggtga
     4621 acacgaacac ctcatcggtg ttgatctcga accgcagcgt gtcgagaaac tcaccggggt
     4681 cttcggtttc cttctgccat tcggtgatct ggcgcaccca ggccaggtca ttgcctgcgc
     4741 cggtggcctt gccctctttg tacttccagt gcgccgccac cccgtattcg gcctgacgat
     4801 gcatctcgtg ggtgcggatc tggaactcca ccggcttgct gtccggcc
//
LOCUS       DAOSUG010000205         4847 bp    DNA     linear   ENV 10-JUN-2024
DEFINITION  MAG TPA_asm: Micropruina sp. isolate AMR_MDS_2195
            NODE_7656_length_4847_cov_3.528172, whole genome shotgun sequence.
ACCESSION   DAOSUG010000205 DAOSUG010000000
VERSION     DAOSUG010000205.1
DBLINK      BioProject: PRJNA925477
            BioSample: SAMN32864262
            Sequence Read Archive: SRR4236673
KEYWORDS    WGS; ENV; Metagenome Assembled Genome; MAG; Third Party Data; TPA;
            TPA:assembly.
SOURCE      Micropruina sp. (wastewater metagenome)
  ORGANISM  Micropruina sp.
            Bacteria; Bacillati; Actinomycetota; Actinomycetes;
            Propionibacteriales; Nocardioidaceae; Micropruina.
REFERENCE   1  (bases 1 to 4847)
  AUTHORS   Abdulkadir,N., Saraiva,J.P., Zhang,J., Stolte,S., Gillor,O.,
            Harms,H. and Rocha,U.
  TITLE     Genome-centric analyses of 165 metagenomes show that mobile genetic
            elements are crucial for the transmission of antimicrobial
            resistance genes to pathogens in activated sludge and wastewater
  JOURNAL   Microbiol Spectr 12 (3), e0291823 (2024)
   PUBMED   38289113
REFERENCE   2  (bases 1 to 4847)
  AUTHORS   Abdulkadir,N.
  TITLE     Direct Submission
  JOURNAL   Submitted (24-MAR-2023) Environmental Microbiology, Helmholtz
            Center for Environmental Research -UFZ, Permoserstr 15, Leipzig
            04318, Germany
COMMENT     The annotation was added by the NCBI Prokaryotic Genome Annotation
            Pipeline (PGAP). Information about PGAP can be found here:
            https://www.ncbi.nlm.nih.gov/genome/annotation_prok/
            
            ##Genome-Assembly-Data-START##
            Assembly Method        :: SPAdes v. 3.11.1
            Genome Representation  :: Full
            Expected Final Version :: Yes
            Genome Coverage        :: Not Applicable
            Sequencing Technology  :: Illumina MiSeq
            ##Genome-Assembly-Data-END##
            
            ##Genome-Annotation-Data-START##
            Annotation Provider               :: NCBI
            Annotation Date                   :: 05/04/2023 04:57:10
            Annotation Pipeline               :: NCBI Prokaryotic Genome
                                                 Annotation Pipeline (PGAP)
            Annotation Method                 :: Best-placed reference protein
                                                 set; GeneMarkS-2+
            Annotation Software revision      :: 6.5
            Features Annotated                :: Gene; CDS; rRNA; tRNA; ncRNA
            Genes (total)                     :: 2,776
            CDSs (total)                      :: 2,750
            Genes (coding)                    :: 2,714
            CDSs (with protein)               :: 2,714
            Genes (RNA)                       :: 26
            tRNAs                             :: 24
            ncRNAs                            :: 2
            Pseudo Genes (total)              :: 36
            CDSs (without protein)            :: 36
            Pseudo Genes (ambiguous residues) :: 0 of 36
            Pseudo Genes (frameshifted)       :: 2 of 36
            Pseudo Genes (incomplete)         :: 32 of 36
            Pseudo Genes (internal stop)      :: 2 of 36
            ##Genome-Annotation-Data-END##
FEATURES             Location/Qualifiers
     source          1..4847
                     /organism="Micropruina sp."
                     /mol_type="genomic DNA"
                     /submitter_seqid="NODE_7656_length_4847_cov_3.528172"
                     /isolate="AMR_MDS_2195"
                     /isolation_source="Activated sludge of municipal
                     wastewater treatment plants"
                     /db_xref="taxon:2737536"
                     /environmental_sample
                     /geo_loc_name="Canada"
                     /lat_lon="49.25 N 123.1 W"
                     /collection_date="2015-06-16"
                     /metagenome_source="wastewater metagenome"
                     /note="metagenomic"
     gene            <1..354
                     /gene="thyA"
                     /locus_tag="PLF56_07735"
     CDS             <1..354
                     /gene="thyA"
                     /locus_tag="PLF56_07735"
                     /EC_number="2.1.1.45"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_017935180.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_function: GO:0004799 - thymidylate synthase activity
                     [Evidence IEA];
                     GO_process: GO:0009265 - 2'-deoxyribonucleotide
                     biosynthetic process [Evidence IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="thymidylate synthase"
                     /protein_id="HOQ53490.1"
                     /translation="AFFQFYVADGRLSCQLYQRSADIFLGVPFNIASYALLTHMVAQV
                     TGLGVGDFVHTLGDAHLYLNHLDQAERQLAREPRALPTLRLNPEVRAIDEFTLADIEV
                     LNYDPHPGIRAPIAV"
     gene            370..1275
                     /locus_tag="PLF56_07740"
     CDS             370..1275
                     /locus_tag="PLF56_07740"
                     /inference="COORDINATES: protein motif:HMM:NF024109.2"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="alpha/beta fold hydrolase"
                     /protein_id="HOQ53491.1"
                     /translation="MTSPVETDFPRATTEAPSRPRSSFPPPIFAPLRRFLTRRRTNPF
                     DRMTVHEFQYGDARVRIYELWTMRPEQAERPDLTTFALVHGLGISSRFFVPLSDALAP
                     YGRVLLFDLPGFHDLPKPRHKMSIVDFADVTKRALDELEVVDPLLVGHSMGAQVVTEM
                     LAEYPGYSSAAMLIGPVVVPDERSLRSVGRRFVQAAVYEPIGNAIAAAGSYLRAGFRW
                     IGDTAPKVVAYPIELRLARSRARVVIVSGEHDAVSPLHWREQLAAEVRGATVLTIPGA
                     AHGVVYNRYAELTDELVALAGIGTA"
     gene            1272..2054
                     /locus_tag="PLF56_07745"
     CDS             1272..2054
                     /locus_tag="PLF56_07745"
                     /inference="COORDINATES: ab initio
                     prediction:GeneMarkS-2+"
                     /note="Derived by automated computational analysis using
                     gene prediction method: GeneMarkS-2+."
                     /codon_start=1
                     /transl_table=11
                     /product="hypothetical protein"
                     /protein_id="HOQ53492.1"
                     /translation="MKRTRAHGSSGRAGGGRAGPSALRVAADVARRTAHGAIDWAFGV
                     GWWVRTQFVRGTPDAFTAPGPDAAHAMPLVLLPGIWERWTFLDPLARALNGYGHPVHL
                     IAALGNNGMPLDASSALVARFLIERDLPEVILVAHSKGGLIGKLVMLDPQVAGRVRGM
                     VALSTPFAGSSLAWPIFKRSPLGIFAPRGRAIVDLAAQREVNARIISLQPTHDQVIPE
                     GSRLEGARNVTLEVAGHFRPLRNPAVHRTIHDLVHELEAQPR"
     gene            2089..2589
                     /locus_tag="PLF56_07750"
     CDS             2089..2589
                     /locus_tag="PLF56_07750"
                     /EC_number="1.5.1.3"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_016456454.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_function: GO:0004146 - dihydrofolate reductase activity
                     [Evidence IEA];
                     GO_process: GO:0046654 - tetrahydrofolate biosynthetic
                     process [Evidence IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="dihydrofolate reductase"
                     /protein_id="HOQ53493.1"
                     /translation="MRIIGIAIVASNGVIGDGQKQPFEIAEDWARFKRVTLGHPMIMG
                     RGTHEAIARWLPGRTTIVVTSDPSRIELPTDGRATGHAVTSVPDALALACSLDDEIYV
                     AGGGSIYRQAWPYLTDLDLTEVQAAAEGTVHFPQVLASEWQQVAREPRDGFALVRYRR
                     LVADQR"
     gene            2589..4004
                     /locus_tag="PLF56_07755"
     CDS             2589..4004
                     /locus_tag="PLF56_07755"
                     /EC_number="2.7.7.7"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_013864531.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_function: GO:0003887 - DNA-directed DNA polymerase
                     activity [Evidence IEA];
                     GO_process: GO:0006260 - DNA replication [Evidence IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="DNA polymerase IV"
                     /protein_id="HOQ53494.1"
                     /translation="MRPQASVLHLDLDAFFAAVEQRDKPSLRGKPVIVGGIGQRGVVS
                     TASYEARVFGVRSAMPMHEARRRCPHAAFLSGRFGAYRAASATVMGLLRELSPLVEPL
                     SLDEAFVDLAVGDRSLAPRDVLAEVATLRASLTERTGGLTASVGVGSSKFIAKVASEL
                     AKPDGVLLVTPGTEVETIAPLPARAIPGIGPVTMQKLSRLGVATVADLQQLSARELVR
                     ELGRSQGDWIGALAFARDDRPVEPDRETKSISVEDTFATDVTDRDALEAVIARDSATV
                     ARRLSAAHLFARTITLKARYPDFTTLSRSRTLDGATDDPAVIALVARALLDQVEVPRG
                     LRLLGVGVAGLTESAQEFLPVEQSCPLVELVESLAVVAGPAEQSGPLVELVETPPAET
                     SPADDRVETTQQPEAQAWNHRQPDGYRPGMDVVHDTHGPGWVWGSGLGRVTVRFETAQ
                     TPPGPVRTFAVDDPALRPASG"
     gene            4172..4253
                     /locus_tag="PLF56_07760"
     tRNA            4172..4253
                     /locus_tag="PLF56_07760"
                     /product="tRNA-Leu"
                     /inference="COORDINATES: profile:tRNAscan-SE:2.0.12"
                     /note="Derived by automated computational analysis using
                     gene prediction method: tRNAscan-SE."
                     /anticodon=(pos:4206..4208,aa:Leu,seq:tag)
     gene            4619..>4847
                     /locus_tag="PLF56_07765"
     CDS             4619..>4847
                     /locus_tag="PLF56_07765"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_011756124.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_component: GO:0110001 - toxin-antitoxin complex
                     [Evidence IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="type II toxin-antitoxin system Phd/YefM family
                     antitoxin"
                     /protein_id="HOQ53495.1"
                     /translation="MTAEPLREVRDHFSDVVDRVDRHHERITVTRNGRPVAVLISPAD
                     LAELEETLDVLSDPDALADIREADAAYASGDV"
BASE COUNT          705 a         1679 c         1659 g          804 t
ORIGIN      
        1 gcgttctttc agttctacgt cgccgacgga cgcctctcgt gccagctcta ccagcgctcg
       61 gccgacatct tcttgggggt tccgttcaac atcgcctcgt acgcattgct gacccacatg
      121 gtcgcgcagg tgaccggttt gggcgtgggt gacttcgtgc acaccctggg ggatgcgcac
      181 ctgtacctca accacctcga ccaggccgag cgccaactgg cacgcgagcc acgtgcgctg
      241 cccacgctgc ggctcaaccc ggaggtgcgc gccatcgatg agttcaccct ggccgacatc
      301 gaggtgctga actacgaccc gcacccgggc atcagggccc cgatcgcggt ttagttcggt
      361 tcagctccca tgaccagtcc cgtcgagacc gacttccctc gcgccaccac cgaggcgccg
      421 agccgtccgc gcagctcgtt tccgcctccg atcttcgcgc cgctgcgtcg ctttctgacc
      481 cgacgccgca ccaacccgtt cgaccgcatg accgtgcacg agtttcagta cggcgacgcc
      541 cgggtgcgca tctacgaact ctggacgatg cgccccgagc aggccgaacg tcccgacctc
      601 accacgttcg cgcttgtgca cggcctgggc atctcgtcgc ggttcttcgt gccgctcagc
      661 gacgcgctcg ccccgtacgg acgggtgctg ctgttcgacc tgcccgggtt tcacgacctg
      721 cccaagccca ggcacaagat gtcgatcgtc gatttcgccg acgtcacgaa gcgagcgctg
      781 gacgagctcg aggtggtcga tccgctgctg gtcgggcact cgatgggcgc gcaggtggtc
      841 accgagatgc ttgccgagta ccccggctac tccagcgcgg cgatgctgat cggcccggtg
      901 gtggtgccgg acgaacgcag cctgcgcagt gtggggcggc ggttcgtcca ggcggccgtc
      961 tatgaaccga tcggcaacgc cattgccgcc gcagggtcgt acctgcgcgc cggttttcgc
     1021 tggatcggcg acaccgcacc caaggtggtc gcctacccga tcgagctgcg cctggcccgc
     1081 tcgcgggcgc gggtggtcat cgtcagcggc gagcacgatg cggtcagccc gttgcattgg
     1141 cgcgagcagt tggcggccga ggtgcgcggc gccaccgtgc tgaccatccc gggcgctgcc
     1201 cacggcgtcg tctacaaccg ctacgccgaa ctcaccgacg agttggtcgc tctggcgggc
     1261 atcggcacgg catgaagcgc acccgggcac acggctcgtc cggacgagcg ggtggtggtc
     1321 gagccggccc gtccgccctg cgcgttgctg cggacgtggc ccggcggacg gcccatggtg
     1381 cgatcgactg ggccttcggg gtcggctggt gggtgcggac gcagttcgtg cgcggaacgc
     1441 cggatgcctt caccgcgccc ggtcccgacg cagcccacgc catgccgctg gtgctgctac
     1501 ccggcatctg ggaacgctgg acgtttcttg atccactggc ccgcgcgctg aacggctacg
     1561 ggcatccggt gcatctgatt gcggctctgg gcaacaacgg gatgccgctg gatgcgtcct
     1621 ccgccctggt cgcccggttt ctgatcgaac gggatctgcc ggaggtgatc ctggtcgcgc
     1681 actccaaggg cgggctgatc ggcaagctgg tgatgctcga cccgcaggtg gccggccggg
     1741 tgcggggcat ggtggcgttg agcacaccgt tcgccgggtc gtccctggcc tggccgatct
     1801 tcaagaggtc gccgctgggc atcttcgcac cccgtgggcg cgcgatcgtc gacctggccg
     1861 cacagcgtga ggtcaacgcc cggatcattt ccctgcaacc cacccacgac caggtgattc
     1921 ccgagggcag ccggctcgag ggtgcccgca acgtaacact cgaggtggcc gggcacttcc
     1981 gtccgctgcg caaccccgcg gtgcatcgca ccattcacga cctggtgcac gaactggagg
     2041 cccagccgcg gtgaagtcgt gtccgacccg gtcggtaggc tcgcctccgt gcggatcatt
     2101 ggcatcgcga tagtcgcctc caacggtgtg atcggcgacg ggcagaagca gccgttcgag
     2161 atcgccgagg actgggcccg gttcaagcgg gtcaccctcg gccacccgat gatcatgggg
     2221 cgcggcacgc acgaggccat cgcacgctgg ctgccgggcc gcaccacgat cgtcgtcacc
     2281 agtgacccga gccgtatcga gttgcccacg gacggtcgcg ccaccggaca cgcggtcacc
     2341 tccgtgcccg atgcactggc gcttgcctgc tccctggacg acgagatcta cgtcgcgggc
     2401 ggcggcagca tctaccgcca ggcctggccc tacctcaccg acctggacct caccgaggtg
     2461 caggccgcgg ccgagggcac ggttcatttt ccgcaggtgc tggccagcga gtggcagcag
     2521 gttgcgcgcg aaccgcgcga cggcttcgcg ttggtccggt accggcgcct ggtcgccgac
     2581 cagcgctgat gcgtccgcag gcctcggttc tgcacctcga cctggacgcc ttcttcgccg
     2641 ccgtcgagca gcgcgacaag ccgtcgctgc gtggcaagcc ggtgatcgtc gggggcatcg
     2701 gccagcgcgg ggtggtgtcg acggcgtcgt acgaggcgcg ggtcttcggg gtgcgctcgg
     2761 ccatgccgat gcatgaggcg cggcgtcgct gcccgcacgc ggcgttcctg tccggacgct
     2821 tcggcgccta ccgggccgcc agcgcgaccg tgatggggtt gctgcgcgaa ctctcgccgc
     2881 tggtcgagcc gctcagcctg gacgaggcct tcgtcgatct ggcggtcggc gaccggtcgc
     2941 tcgcaccgcg tgacgtgctg gccgaggtgg cgaccctgcg ggcttcgttg accgagcgca
     3001 ccgggggcct gaccgcctcg gtgggcgtcg gatcgtccaa gttcatcgcg aaggtggcca
     3061 gcgaactggc caagccggac ggagtgctgc tggtcacccc gggcaccgag gtcgagacca
     3121 tcgcgccgct gccggcccgg gcgattccgg gcatcggccc ggtgaccatg cagaagctgt
     3181 cccggctggg ggtggcgacg gtggccgacc tgcagcaact gtcggcccgc gaactggtgc
     3241 gcgagctggg ccggtcgcag ggggactgga tcggggcgct ggccttcgcg cgcgacgacc
     3301 gtccggtcga gcccgaccgc gagaccaagt cgatctcggt ggaggacacg ttcgccaccg
     3361 acgtcaccga tcgcgacgcg ctggaggcgg tgattgcgcg cgactcggcg accgtggccc
     3421 ggcgactgtc ggcggcgcac ctgttcgccc gcacgatcac gctcaaggcg cgctaccccg
     3481 acttcaccac gctgtcgcgc tcacgcaccc tggacggcgc gaccgacgac ccggcggtga
     3541 tcgccctggt ggcgcgggca ttgctcgacc aggtcgaggt gccccgcggg ttgcggttgc
     3601 tgggtgtggg ggtggccggg ctcaccgagt cggcccagga gttccttccg gtcgagcagt
     3661 cctgtccgct ggtcgagctt gtcgagagcc tggcggtcgt tgcaggtccg gcggagcagt
     3721 ccggtccgtt ggtcgagctt gtcgaaaccc ctccggccga gacctcgccc gccgatgatc
     3781 gcgtggagac cacgcagcag cccgaagcgc aggcctggaa ccaccgccag cccgacggct
     3841 accggccagg catggacgtg gtgcacgaca cccacggccc gggctgggtg tggggcagcg
     3901 ggcttggtcg ggtgaccgtc cggttcgaga ccgcgcagac cccgccgggg cccgtccgca
     3961 ccttcgccgt ggacgacccg gcgctccgtc cggcaagcgg ctgaaacgtc atccgccgac
     4021 gagaaccgcg atcggccggc atcggggtgt gccctccgcg gctcgtggca gcggggatcg
     4081 aagcacgtgg ggcgttcgac ccctgtcgtg cagcatgctc gcccactgcg atcgggcggc
     4141 gctctcgtcc ggctctacac tggcctgtgc cgccggagtg gtgaaattgg cagacacgca
     4201 ggatttaggt tcctgtgctt tcgggcgtga gggttcaagt cccttctccg gcaccgtcaa
     4261 cctcgagccg taccatggtt gtcaataggt cagctaaaca cacaccggaa atgtccctca
     4321 agccccgaaa accgtcgcaa gttacccaca ggcgctgggc taaaccccta gccaaacaga
     4381 tgtccccccc gaaagttctg agacatcccc ttgacggtgc ccggatggca agttcggcgc
     4441 cggtcgagcg acgtcagatg gaccgccgga cacagccggc cacatccgca aaaacgtttc
     4501 acgagcgttg gtgcggcacg gtcaccttga gcaggcaggc tgatctctcg atcccaggga
     4561 ctgcacggtc catggacggg tgcttgactc tgcgtgaatg agcgccacaa ttgtggtcat
     4621 gactgctgag ccgctgcgtg aggtgcgtga ccacttcagt gacgttgtgg accgtgtcga
     4681 tcgccatcac gaacgaatca ccgtcacccg caacggacgg ccggtcgcgg tgctgatcag
     4741 cccagctgac ctggctgagt tggaggagac gctcgatgtg ctgtccgacc ccgacgctct
     4801 ggctgatatt cgtgaagccg atgccgccta cgccagcggc gatgtgg
//
LOCUS       DAOSUG010000206         4784 bp    DNA     linear   ENV 10-JUN-2024
DEFINITION  MAG TPA_asm: Micropruina sp. isolate AMR_MDS_2195
            NODE_7751_length_4784_cov_3.588074, whole genome shotgun sequence.
ACCESSION   DAOSUG010000206 DAOSUG010000000
VERSION     DAOSUG010000206.1
DBLINK      BioProject: PRJNA925477
            BioSample: SAMN32864262
            Sequence Read Archive: SRR4236673
KEYWORDS    WGS; ENV; Metagenome Assembled Genome; MAG; Third Party Data; TPA;
            TPA:assembly.
SOURCE      Micropruina sp. (wastewater metagenome)
  ORGANISM  Micropruina sp.
            Bacteria; Bacillati; Actinomycetota; Actinomycetes;
            Propionibacteriales; Nocardioidaceae; Micropruina.
REFERENCE   1  (bases 1 to 4784)
  AUTHORS   Abdulkadir,N., Saraiva,J.P., Zhang,J., Stolte,S., Gillor,O.,
            Harms,H. and Rocha,U.
  TITLE     Genome-centric analyses of 165 metagenomes show that mobile genetic
            elements are crucial for the transmission of antimicrobial
            resistance genes to pathogens in activated sludge and wastewater
  JOURNAL   Microbiol Spectr 12 (3), e0291823 (2024)
   PUBMED   38289113
REFERENCE   2  (bases 1 to 4784)
  AUTHORS   Abdulkadir,N.
  TITLE     Direct Submission
  JOURNAL   Submitted (24-MAR-2023) Environmental Microbiology, Helmholtz
            Center for Environmental Research -UFZ, Permoserstr 15, Leipzig
            04318, Germany
COMMENT     The annotation was added by the NCBI Prokaryotic Genome Annotation
            Pipeline (PGAP). Information about PGAP can be found here:
            https://www.ncbi.nlm.nih.gov/genome/annotation_prok/
            
            ##Genome-Assembly-Data-START##
            Assembly Method        :: SPAdes v. 3.11.1
            Genome Representation  :: Full
            Expected Final Version :: Yes
            Genome Coverage        :: Not Applicable
            Sequencing Technology  :: Illumina MiSeq
            ##Genome-Assembly-Data-END##
            
            ##Genome-Annotation-Data-START##
            Annotation Provider               :: NCBI
            Annotation Date                   :: 05/04/2023 04:57:10
            Annotation Pipeline               :: NCBI Prokaryotic Genome
                                                 Annotation Pipeline (PGAP)
            Annotation Method                 :: Best-placed reference protein
                                                 set; GeneMarkS-2+
            Annotation Software revision      :: 6.5
            Features Annotated                :: Gene; CDS; rRNA; tRNA; ncRNA
            Genes (total)                     :: 2,776
            CDSs (total)                      :: 2,750
            Genes (coding)                    :: 2,714
            CDSs (with protein)               :: 2,714
            Genes (RNA)                       :: 26
            tRNAs                             :: 24
            ncRNAs                            :: 2
            Pseudo Genes (total)              :: 36
            CDSs (without protein)            :: 36
            Pseudo Genes (ambiguous residues) :: 0 of 36
            Pseudo Genes (frameshifted)       :: 2 of 36
            Pseudo Genes (incomplete)         :: 32 of 36
            Pseudo Genes (internal stop)      :: 2 of 36
            ##Genome-Annotation-Data-END##
FEATURES             Location/Qualifiers
     source          1..4784
                     /organism="Micropruina sp."
                     /mol_type="genomic DNA"
                     /submitter_seqid="NODE_7751_length_4784_cov_3.588074"
                     /isolate="AMR_MDS_2195"
                     /isolation_source="Activated sludge of municipal
                     wastewater treatment plants"
                     /db_xref="taxon:2737536"
                     /environmental_sample
                     /geo_loc_name="Canada"
                     /lat_lon="49.25 N 123.1 W"
                     /collection_date="2015-06-16"
                     /metagenome_source="wastewater metagenome"
                     /note="metagenomic"
     gene            <1..1699
                     /gene="sepH"
                     /locus_tag="PLF56_07770"
     CDS             <1..1699
                     /gene="sepH"
                     /locus_tag="PLF56_07770"
                     /inference="COORDINATES: protein motif:HMM:NF040712.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_process: GO:0051301 - cell division [Evidence IEA]"
                     /codon_start=2
                     /transl_table=11
                     /product="septation protein SepH"
                     /protein_id="HOQ53496.1"
                     /translation="PLGLSPDGRNLIVVTDGGEQVAIPADDRLRAALRNDRARMGQLE
                     IEMESALRPRDIQARIRAGETLESVAQAAGVPSQQIEPFAAPVIAEREHIAGLAQANP
                     VRRAGDAVAHRSLRAVVTDRLLSRGVDVDTVDWDAWRNEDRRWTVRLSYESGSAHRQA
                     DFTYDQTGRFSLAANDDARWLTGEHSSSHGPQPGRRRPQDSDDESTADLNDELAIVRA
                     IQPQFAVTPDIDDELDEPDRPDDLDDENSTPDNEDAFAEGDLAEVNGVYDIIPGDRSN
                     MDVLYDMLSSFDEDSVQIYAGLVRPAPDEAAAPVIDPGAGNVEAIVVETVSVIEVLPQ
                     ESVTDDEDRLPDDEQGGEAVVGVDHTDDPAGAEAHGSEPPRDAGASDDVDDDEFLDDD
                     DDESLFAEVPIVHPVRADVPLLTEPDDDDGGLASPVEAEAITEVIEPAQPHHEPVGSA
                     ETATPPPSEPVGVSSHEGTDDNATPGREPAGSSAHPSDPPQPEQPEQPAEPPRRPTGR
                     RRPVTPAATEPEQPRLVDEAGAEPSRPIRRKRASVPSWDEIMFGAPAPRPDQSTPTD"
     gene            1713..2795
                     /locus_tag="PLF56_07775"
     CDS             1713..2795
                     /locus_tag="PLF56_07775"
                     /inference="COORDINATES: ab initio
                     prediction:GeneMarkS-2+"
                     /note="Derived by automated computational analysis using
                     gene prediction method: GeneMarkS-2+."
                     /codon_start=1
                     /transl_table=11
                     /product="hypothetical protein"
                     /protein_id="HOQ53497.1"
                     /translation="MRHLRVVVTGLLIAGSAVLAACSGGTAATPSAPEPQTAITARVL
                     GQLLDQHDPGSYAIAPSDAPVAGSEPTEFISGLSATAYYRATPGRAARTLTLSYAPAR
                     YAGTFDCAEPTCIRRDGVLVRERSHEVISVREVGFVRVSGSPSNFEGDALDAVIALAR
                     DATIAPTVGQSLAHAAAANPRWRSDALDCGDASPSAPIALPAAAGAVRAVTPQALAAV
                     LASHLAASCAGDDSRSDTITGTVYLGSDTERVSLTIAATPYRCPQLDTCRTVNGLTVA
                     EQHDVAEQTYPAHLVVARQLPDGTHWLIADEASVRAHNGRFPVDLSGLTQVVNDPRVG
                     AGVDAALISAGDDLPLRWRLAPRTSE"
     gene            complement(2814..3458)
                     /locus_tag="PLF56_07780"
     CDS             complement(2814..3458)
                     /locus_tag="PLF56_07780"
                     /EC_number="2.7.1.21"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_014846562.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_function: GO:0004797 - thymidine kinase activity
                     [Evidence IEA];
                     GO_function: GO:0005524 - ATP binding [Evidence IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="thymidine kinase"
                     /protein_id="HOQ53498.1"
                     /translation="MAQLIFFTGPMNCGKSTLALQTDFTEAQAGRSGRLFTRDDRAGS
                     AMISSRIGLARPAVEVGDAFDFWAYVIAELTSARRVDYLICDEAQFYTGAHIDQLARV
                     VDELQLDVYCFGILTDFRTELFPATRRLVELCDRMELLQVRPRCWCGEPATHNARTVN
                     GRMVTEGSQVVVGDVAAEQGQVAYEVLCRRHHRRRMTAAVARITLNPEPLPFDE"
     gene            complement(3462..4577)
                     /locus_tag="PLF56_07785"
     CDS             complement(3462..4577)
                     /locus_tag="PLF56_07785"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_016669981.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="alkaline phosphatase family protein"
                     /protein_id="HOQ53499.1"
                     /translation="MSQPLVPAYGTSTLADLLPSVAAVLGLPGRDNVLALPPMQRFVV
                     LLVDGLGWHLLDGREAIAPYLCGLRAQARPITAGVPSTTATSITSLGTGLAPGQHGVA
                     GYTFRFGGGLLNALVWQEGLSGLDVQPQLTVFERLAKTGVHCSTVTPARFQGSGLTTC
                     ALRGANFLGVSDQAAAERRVELAVQASEAGERSLVYVYDRDLDHAGHKAGVASPAWAD
                     ELARIDRFARQLRAALHESVGLIITGDHGMVDVPLDQRLSIEQHDELTADVQLIGGEG
                     RLRQLYVRPGTEAAVQARWADRLGAEAWVLTRGEASDAGWFGPIAPRLADRFGDVVVA
                     MAGQGALMTQTQPNEYTLVGMHGSLTAAELTVPLLIG"
     gene            complement(4574..>4784)
                     /locus_tag="PLF56_07790"
     CDS             complement(4574..>4784)
                     /locus_tag="PLF56_07790"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_015070337.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=2
                     /transl_table=11
                     /product="DUF5998 family protein"
                     /protein_id="HOQ53500.1"
                     /translation="SVGWGTATRVEIEPAHCGDPDCDADHGYSGTVAADDLTVRMSVA
                     ADGADNVARLVSFATALQVASGGGR"
BASE COUNT          745 a         1750 c         1648 g          641 t
ORIGIN      
        1 tcccctgggg ctgagccccg acggacgcaa cctgatcgtc gtcaccgatg gcggcgagca
       61 ggtggcgatc ccggccgatg accggctgcg ggccgcgctg cgcaacgacc gagcacgcat
      121 gggacaactg gagatcgaga tggaaagtgc actgcggccg cgcgacatcc aggcccggat
      181 cagggccggg gagacgctgg agtcggtggc gcaggccgcc ggggtgccca gtcagcagat
      241 cgagccgttc gcggctccgg tgatcgccga gcgggaacac atcgcgggcc tggcccaggc
      301 caaccccgtc cgccgggccg gtgacgccgt tgcgcaccgc agtctgcgcg ccgtggtcac
      361 cgaccggttg ctgtcacggg gggtcgacgt cgacaccgtc gactgggacg cctggcgcaa
      421 cgaggatcgg cgctggaccg tccggttgtc gtatgaatcg ggttcggcgc accgtcaggc
      481 cgacttcacc tacgaccaga ccggacgctt ctcgctcgcc gccaacgacg acgcgcgctg
      541 gctgaccggc gaacactcga gttcgcacgg tccccagccc ggccgcaggc ggccacagga
      601 ttccgacgac gagagcaccg ccgacctcaa cgacgagttg gcgatcgtgc gtgcgatcca
      661 gccccagttc gccgtcacgc ccgacatcga cgacgagctg gacgagccgg accggcccga
      721 cgacctggac gacgagaaca gcacccccga caacgaggat gccttcgccg agggtgacct
      781 ggccgaggtg aacggcgtct acgacatcat tcccggcgac cgctccaaca tggacgtgct
      841 gtacgacatg ctgtcgagct tcgacgagga ttcggtgcag atctatgcgg gcctggtccg
      901 gccggccccc gacgaggctg cggcgccggt gatcgacccg ggcgccggga acgtcgaggc
      961 gatcgtggtc gagacggtca gcgtgatcga ggtgctgccc caggagtcgg tcaccgacga
     1021 cgaggaccgc ctgccggatg acgagcaggg cggcgaagca gtggtcggcg tcgaccacac
     1081 cgacgatccc gcaggagccg aagcacacgg ctccgaacct ccgcgcgatg ccggagcgtc
     1141 ggacgacgtc gacgacgacg agttcctgga cgacgacgat gacgagtcgc tcttcgccga
     1201 ggtgcccatc gtgcaccccg tccgagccga tgtgcccctg ctcaccgaac cggacgacga
     1261 cgacggcggc ctggcctcgc cggtcgaagc cgaggcgatc accgaggtga tcgagcccgc
     1321 gcagccgcac cacgagccgg tcggcagcgc cgaaacagcc actcccccac cgtcggaacc
     1381 ggtgggggtc tcgagccatg agggtaccga cgacaatgcg actcctgggc gcgaaccggc
     1441 aggatcgtca gcccacccgt ccgacccgcc gcagcccgag caacccgagc aacccgccga
     1501 accgccccgg cgtccgaccg gccggcgacg ccccgtgacg ccggccgcaa cagagccgga
     1561 gcagccccgc ctggtcgacg aggcgggggc cgagccctcg cggccgatcc ggcgcaagcg
     1621 cgcgtcggtg cccagttggg acgagatcat gttcggtgca ccggcgccgc gtcccgatca
     1681 gtccacaccg acggactgag gggggcctgg ctttgcgcca ccttcgcgtc gtcgtcaccg
     1741 gcctgctgat cgcagggtcg gcggtactgg cggcctgctc gggaggtacc gcggccacgc
     1801 cgtcggcccc ggaaccccag accgccatca ctgcacgggt gctgggccag ttgctcgacc
     1861 agcacgaccc cggctcgtac gccatcgcgc ccagcgacgc gcctgtggcc ggatccgaac
     1921 cgaccgagtt catctcaggc ctcagcgcga cggcgtatta ccgggcaact cccggacgcg
     1981 ccgccagaac cctcacgctc agctacgcgc cggcacgcta tgcgggcacg ttcgactgtg
     2041 ccgagcccac ctgcatccgg cgcgacggtg tgctcgtgcg cgagcggtca cacgaggtga
     2101 tctcggtgcg cgaagtgggt ttcgtccggg tgtccggttc gccgtcgaac ttcgaaggtg
     2161 acgccctcga cgccgtgatc gcattggctc gtgatgccac catcgcgccc accgtcgggc
     2221 agtccctggc gcacgcggca gcagccaacc cgcgctggcg cagcgacgcg ctggactgcg
     2281 gcgacgcgtc gccctcggct cccatcgccc tgcctgctgc tgccggcgcg gtgcgggccg
     2341 tcacgcctca ggccctggcc gccgtgctgg ccagtcacct ggccgcctcg tgcgccggtg
     2401 acgacagccg tagcgacacg atcaccggaa ccgtctatct gggttcggac accgagcggg
     2461 tcagcctgac catcgccgcg acgccctacc ggtgccccca actggacacc tgccgcaccg
     2521 tgaacggtct cacggtcgcc gaacagcacg atgtggcaga gcagacctac cccgcccatc
     2581 tggtcgtggc caggcaactg cccgacggca cccactggtt gatcgccgac gaggccagcg
     2641 ttcgtgcgca caacggccgg tttccggtgg acctgagcgg tctcacgcag gtggtgaacg
     2701 atccccgggt gggggccggc gtcgatgcgg cactgatcag tgccggcgac gacctgccgc
     2761 tgcggtggcg gctggcaccg cgcacgtccg agtgagcccc gtcgtgggcc gcgtcactcg
     2821 tcgaacggca gcggctcggg attcagcgtg atccgggcca ccgccgcagt catccggcgc
     2881 cggtggtgcc gtcggcagag cacctcgtag gccacctggc cctgctcggc ggccacgtcg
     2941 cccaccacca cctggctgcc ctcggtgacc atgcgcccgt tcaccgtgcg ggcgttgtgg
     3001 gtggccggtt cgccgcacca gcaccgcgga cgcacctgca acaactccat gcggtcgcac
     3061 aactccacca gtcgccgggt ggcgggaaac agttcggtgc gaaagtcggt gagaatgccg
     3121 aagcagtaca cgtccagttg aagttcgtcc accacgcggg ccaactggtc gatgtgcgcc
     3181 ccggtgtaga actgggcctc gtcgcagatc aggtagtcca cgcggcgcgc cgaggtgagc
     3241 tcggcgatca cgtacgccca aaagtcgaac gcatcaccca cctcgacggc cggccgcgcc
     3301 agcccgatcc gcgacgagat catggccgac ccggcacggt cgtcgcgggt gaacagtcgc
     3361 ccggagcgtc cggcctgcgc ctccgtgaag tcggtctgca gggccagcgt cgacttgccg
     3421 cagttcatcg ggccggtgaa gaagatcagt tgcgccacgg ctcagccgat cagcagcggc
     3481 acggtcagtt cggccgccgt cagcgatccg tgcatgccga ccaatgtgta ctcgttgggc
     3541 tgggtctgcg tcatcagcgc accctgcccc gccatggcca ccaccacgtc gccgaagcgg
     3601 tcggccagcc gcggtgcgat aggcccgaac cagccggcat cggacgcctc gccgcgggtc
     3661 agcacccagg cctcggcccc cagtcgatcg gcccagcggg cctggacggc agcctcggtg
     3721 cccggccgca cgtacagctg gcgcagccgt ccctcgccgc cgatgagctg gacgtcggcg
     3781 gtcaactcgt cgtgctgttc gatgctgagc cgctggtcga gcggcacgtc gaccatgccg
     3841 tgatcaccgg tgatgatcaa ccccaccgac tcgtgcagcg ccgcgcgcag ctgccgggcg
     3901 aaccggtcga tgcgggcgag ttcgtccgcc caggccggtg atgccacccc ggccttgtgg
     3961 ccggcatggt cgaggtcgcg gtcgtacacg tagaccaggc tgcgctcgcc ggcctcggac
     4021 gcttgcaccg ccagttcgac ccggcgctcc gcagcggcct ggtcgctcac gccgagaaag
     4081 ttcgcgccgc gcaacgcgca ggtggtcagg ccgctgccct ggaaccgggc cggtgtcacg
     4141 gtgctgcagt gcaccccggt cttggccagg cgttcgaaga cggtgagctg cggttgcacg
     4201 tccaggccgc tcaggccttc ctgccacacc agcgcattca gcagcccgcc gccgaagcgg
     4261 aaggtgtagc cggccacgcc gtgctgcccc ggcgccagcc ccgtgccgag gctagtgatg
     4321 gacgtcgccg tggtgctcgg cactccggcc gtgatcggac gcgcctgcgc gcgcaggccg
     4381 cacaggtagg gggcgatcgc ctcgcggccg tcgagcaggt gccagcccag gccgtccacc
     4441 agcagcacca cgaaacgctg catcggcggc agcgccagca cgttgtcgcg gccgggcagg
     4501 ccgagcaccg cggcaacgct gggcagcaga tctgcgagcg tcgacgtgcc gtaggccggc
     4561 acgagcggtt ggctcaccgg ccaccgccgc tggccacctg cagggcggtg gcgaacgaca
     4621 ccaggcgggc cacgttgtcg gcgccatcgg cggccacgct catgcggacg gtcaggtcgt
     4681 cggcggccac ggtgcccgaa tagccgtgat cggcgtcgca gtcgggatcg ccgcagtgcg
     4741 cgggctcgat ctcgacccgg gtggcggtgc cccagccgac gctg
//
LOCUS       DAOSUG010000207         4760 bp    DNA     linear   ENV 10-JUN-2024
DEFINITION  MAG TPA_asm: Micropruina sp. isolate AMR_MDS_2195
            NODE_7796_length_4760_cov_3.272901, whole genome shotgun sequence.
ACCESSION   DAOSUG010000207 DAOSUG010000000
VERSION     DAOSUG010000207.1
DBLINK      BioProject: PRJNA925477
            BioSample: SAMN32864262
            Sequence Read Archive: SRR4236673
KEYWORDS    WGS; ENV; Metagenome Assembled Genome; MAG; Third Party Data; TPA;
            TPA:assembly.
SOURCE      Micropruina sp. (wastewater metagenome)
  ORGANISM  Micropruina sp.
            Bacteria; Bacillati; Actinomycetota; Actinomycetes;
            Propionibacteriales; Nocardioidaceae; Micropruina.
REFERENCE   1  (bases 1 to 4760)
  AUTHORS   Abdulkadir,N., Saraiva,J.P., Zhang,J., Stolte,S., Gillor,O.,
            Harms,H. and Rocha,U.
  TITLE     Genome-centric analyses of 165 metagenomes show that mobile genetic
            elements are crucial for the transmission of antimicrobial
            resistance genes to pathogens in activated sludge and wastewater
  JOURNAL   Microbiol Spectr 12 (3), e0291823 (2024)
   PUBMED   38289113
REFERENCE   2  (bases 1 to 4760)
  AUTHORS   Abdulkadir,N.
  TITLE     Direct Submission
  JOURNAL   Submitted (24-MAR-2023) Environmental Microbiology, Helmholtz
            Center for Environmental Research -UFZ, Permoserstr 15, Leipzig
            04318, Germany
COMMENT     The annotation was added by the NCBI Prokaryotic Genome Annotation
            Pipeline (PGAP). Information about PGAP can be found here:
            https://www.ncbi.nlm.nih.gov/genome/annotation_prok/
            
            ##Genome-Assembly-Data-START##
            Assembly Method        :: SPAdes v. 3.11.1
            Genome Representation  :: Full
            Expected Final Version :: Yes
            Genome Coverage        :: Not Applicable
            Sequencing Technology  :: Illumina MiSeq
            ##Genome-Assembly-Data-END##
            
            ##Genome-Annotation-Data-START##
            Annotation Provider               :: NCBI
            Annotation Date                   :: 05/04/2023 04:57:10
            Annotation Pipeline               :: NCBI Prokaryotic Genome
                                                 Annotation Pipeline (PGAP)
            Annotation Method                 :: Best-placed reference protein
                                                 set; GeneMarkS-2+
            Annotation Software revision      :: 6.5
            Features Annotated                :: Gene; CDS; rRNA; tRNA; ncRNA
            Genes (total)                     :: 2,776
            CDSs (total)                      :: 2,750
            Genes (coding)                    :: 2,714
            CDSs (with protein)               :: 2,714
            Genes (RNA)                       :: 26
            tRNAs                             :: 24
            ncRNAs                            :: 2
            Pseudo Genes (total)              :: 36
            CDSs (without protein)            :: 36
            Pseudo Genes (ambiguous residues) :: 0 of 36
            Pseudo Genes (frameshifted)       :: 2 of 36
            Pseudo Genes (incomplete)         :: 32 of 36
            Pseudo Genes (internal stop)      :: 2 of 36
            ##Genome-Annotation-Data-END##
FEATURES             Location/Qualifiers
     source          1..4760
                     /organism="Micropruina sp."
                     /mol_type="genomic DNA"
                     /submitter_seqid="NODE_7796_length_4760_cov_3.272901"
                     /isolate="AMR_MDS_2195"
                     /isolation_source="Activated sludge of municipal
                     wastewater treatment plants"
                     /db_xref="taxon:2737536"
                     /environmental_sample
                     /geo_loc_name="Canada"
                     /lat_lon="49.25 N 123.1 W"
                     /collection_date="2015-06-16"
                     /metagenome_source="wastewater metagenome"
                     /note="metagenomic"
     gene            <1..155
                     /locus_tag="PLF56_07795"
     CDS             <1..155
                     /locus_tag="PLF56_07795"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_002536538.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=3
                     /transl_table=11
                     /product="HAD hydrolase-like protein"
                     /protein_id="HOQ53501.1"
                     /translation="RMDTDVVAGMEAGLHTVLVLTGVTTEADVATYPFRPNQILNGVH
                     ELIEQI"
     gene            683..1738
                     /gene="argC"
                     /locus_tag="PLF56_07800"
     CDS             683..1738
                     /gene="argC"
                     /locus_tag="PLF56_07800"
                     /EC_number="1.2.1.38"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_020577492.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_function: GO:0003942 -
                     N-acetyl-gamma-glutamyl-phosphate reductase activity
                     [Evidence IEA];
                     GO_process: GO:0006592 - ornithine biosynthetic process
                     [Evidence IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="N-acetyl-gamma-glutamyl-phosphate reductase"
                     /protein_id="HOQ53502.1"
                     /translation="MSVRIAVAGCTGYAGGEALRLLLQHPQVSIGNLTAKSSAGSLVG
                     EHQPHLRPLAATRVLDTTVEHLAGHDVIFLALPHGASGAIAAQLAKASPDSLLIDLGA
                     DHRLESAEAWTAYYGGEHPGTWTYGMPELPLANGLRQREPLRTTRRIAVPGCNVTAVT
                     FALAPGIAAGLIEPTDLVAVLANGVSGAGKALKPHLLASEILGSASPYAVGGSHRHIP
                     EIEQNLGKSRGDAIRISFTPTLVPMSRGILATCTARLAEGADPATVRQAWADAYAGEP
                     FVQLLPEGQWPATATTLGANTALVQVTVDQRAGRLVAVCAIDNLVKGTAGAAVQSMNL
                     ALGLPETTGLTSIGVAP"
     gene            1735..2889
                     /gene="argJ"
                     /locus_tag="PLF56_07805"
     CDS             1735..2889
                     /gene="argJ"
                     /locus_tag="PLF56_07805"
                     /EC_number="2.3.1.1"
                     /EC_number="2.3.1.35"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_016670090.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_function: GO:0004358 - glutamate N-acetyltransferase
                     activity [Evidence IEA];
                     GO_process: GO:0006526 - arginine biosynthetic process
                     [Evidence IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="bifunctional glutamate
                     N-acetyltransferase/amino-acid acetyltransferase ArgJ"
                     /protein_id="HOQ53503.1"
                     /translation="MSVTTPKGFTAAGATAGIKASGRPDLAIVVNNGPDAHAAAVFTS
                     NAFQAAPVLWSREAVADGRLAAVVLNSGGANACTGTAGLEDARQMAAWTSEALGTDVT
                     DVAVCSTGLIGVRLPMDKVLAGIELATQDLSEVGGPAAAEAIMTTDTVPKQAEFHSQQ
                     RWSIGGMAKGAGMLAPALATMLVVLTTDAVIAAADLDTALRTATSFTFDRTDSDGCMS
                     TNDTVIVLASGASGVSADPEEFTEALTGVCHSLAMQLLADSEGSAHDIEVTVHGAASE
                     SDALAMARAIARSNLFKCAVFGNDPNWGRVLAAAGTVRARFDPDQVDVSFNDVGVFVG
                     GQLGADRSEVDLSERQVVVEVNLHAGDASATIWTNDLTYDYVKENAEYST"
     gene            2886..3812
                     /gene="argB"
                     /locus_tag="PLF56_07810"
     CDS             2886..3812
                     /gene="argB"
                     /locus_tag="PLF56_07810"
                     /EC_number="2.7.2.8"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_020391013.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_function: GO:0003991 - acetylglutamate kinase activity
                     [Evidence IEA];
                     GO_process: GO:0006592 - ornithine biosynthetic process
                     [Evidence IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="acetylglutamate kinase"
                     /protein_id="HOQ53504.1"
                     /translation="MSPTTRTRATAEGVIGKAETLIEALPWLERYAGSIVVVKYGGNA
                     MVNDELKRAFADDIVFLRRCGLKPVVVHGGGPQISSMLGRLGIASEFKGGLRVTSPEA
                     MDVVRMVLVGQVGRELVGLLNAHGPLAVGLSGEDAGLFTATKRGVTIDGEQVDLGLVG
                     EVTTVRPETVIDLIEGGRIPVIATVAPDEDGHVHNVNADTAAGALAVALGAERFLVLT
                     DVEGLYADWPASEEVIARITTAELAELLPSLEAGMIPKMEACLQAVEGGVRRATIIDG
                     RVPHSLLLEIFTSEGLGTMVVAPDDTATGVLA"
     gene            4025..>4760
                     /locus_tag="PLF56_07815"
     CDS             4025..>4760
                     /locus_tag="PLF56_07815"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_014847020.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_function: GO:0008483 - transaminase activity [Evidence
                     IEA];
                     GO_function: GO:0030170 - pyridoxal phosphate binding
                     [Evidence IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="aminotransferase class III-fold pyridoxal
                     phosphate-dependent enzyme"
                     /protein_id="HOQ53505.1"
                     /translation="MNTFGPPKRVFVRGEGVHLWDADGNRYLDLLAGLAVNSLGHAHP
                     QVIGAISAQLSTLGHVSNFFATPTQIALAERLCGFVTLNDPGREARVFFTNSGTEANE
                     AGFKITRRTGRTRLIAMQGSFHGRSLGALALTSNPAYREPFEPLPGEVTFVPYGDADA
                     LAQALDDTVAAVILEPIQGENGVVEPPSDYLAAVRALTTNAGALLWIDEVQTGIGRCG
                     EWLASAHSGVTGDIVTFAKGLGNGFPV"
BASE COUNT          750 a         1739 c         1536 g          735 t
ORIGIN      
        1 accgcatgga caccgacgtc gtcgcaggca tggaggccgg cctgcacacc gtgctggtgc
       61 tgacgggtgt gaccaccgag gccgacgttg cgacgtaccc cttccgtccc aaccagattc
      121 tgaacggcgt ccacgagttg atcgagcaga tctagggtga actggacgga atccgtccac
      181 cgttggtcga gtgggagacc ctgcctccct catcccgcga accctctccc gtcatctcgg
      241 ccaaggccgg gatgtcctca agcggccctg tcgtcatccc gcgagccctc tcccgtcatc
      301 ccgcgaacac aatccccgtc atcccggcga acgccgggat cttctcaggt gcctgccaat
      361 gacgcgggaa ctccgctggt cgagaccgct ctcccgctgg gttgcgtttg ctcccgcgga
      421 atgagggtca gccacctgcg tcgccggtgt gcattagtgg gtgggttttg ccaccgcagt
      481 cggtgccgaa acccacccac tctttcggat ccggccagca ggccgctgga gaaggcggct
      541 cgcccgatga tccgggtggt tgagggctga atggagacct cgcccggtca tggacgtgtc
      601 ggtggccgcc ttcgttccca acccctccga tcgcctcaca gccggttgga atattcattc
      661 cgaaaagggc atactcattc ccatgtctgt tcgtatcgcc gtggccggct gcacggggta
      721 tgccggcggc gaggccctgc gcctgctgct gcagcatccc caggtctcca tcggcaacct
      781 gaccgcgaaa tcgagtgccg gctcactggt gggcgagcac cagccccacc tgcgaccgct
      841 ggccgctacc cgcgtgctcg acacgaccgt cgagcacctg gccggtcacg atgtgatctt
      901 tctggcgctg ccgcacggcg cctccggtgc gatcgccgcc cagctcgcca aggcctcgcc
      961 ggactcgttg ttgatcgatc tgggcgccga ccaccggctc gaatccgccg aggcatggac
     1021 tgcctattac ggcggcgagc accccggcac ctggacctac ggcatgcccg aactgccgct
     1081 ggcgaacgga ctccgccaac gtgagcccct gcgcaccacg cgtcgaatcg ccgtaccggg
     1141 gtgcaatgtc accgccgtca ccttcgcgct cgcgccgggc atcgccgccg gtttgatcga
     1201 acccaccgac ctggtcgccg tgctggccaa cggggtcagc ggcgccggca aggctctcaa
     1261 gccgcacctg ctggcgtccg agattctggg ctcggcaagc ccgtacgcgg tcggcggcag
     1321 ccaccgacac atccccgaga tcgaacagaa tctcggcaag agcaggggcg acgccatccg
     1381 gatcagcttc acgcccaccc tggtgccgat gtcccgcggc atcctggcca cctgtacggc
     1441 ccggcttgcc gaaggcgccg atccggccac cgtccggcag gcctgggccg atgcctacgc
     1501 cggcgaaccc ttcgtgcagt tgctgcccga ggggcagtgg cccgccaccg ccaccacgct
     1561 gggtgccaac acggctctcg tccaggtcac cgtcgatcag cgcgccggac gcctggtcgc
     1621 cgtctgcgcg atcgacaatc tggtcaaggg caccgcaggc gccgccgtcc agtccatgaa
     1681 cctggccctc ggcctgcccg agacaaccgg cctcacctcg atcggagtcg caccatgagc
     1741 gtcaccaccc ccaagggttt caccgccgcc ggcgccacgg cgggcatcaa ggcgtccggt
     1801 cgccccgacc tggcgatcgt ggtcaacaac ggtccggacg cgcacgctgc cgcggtcttc
     1861 acctccaacg cctttcaggc cgcacccgtg ctgtggtcgc gcgaagccgt cgccgacggt
     1921 cgtctcgctg cagtcgtgct caactccggc ggcgccaatg cctgcacggg tacggccgga
     1981 ctcgaggatg cgcggcagat ggccgcgtgg acgtccgagg cgctcggtac cgacgtcacc
     2041 gacgtggcgg tgtgctcgac cgggctgatc ggcgtccggt tgccgatgga caaggtgctc
     2101 gccggcatcg aactcgccac ccaggacctc tccgaggttg gcggccccgc cgcggcggag
     2161 gccatcatga ccaccgacac cgtccccaag caggccgagt tccactcgca gcagcgctgg
     2221 tcgatcggcg gcatggccaa gggggcgggc atgctggccc cggccctggc caccatgctc
     2281 gtggtgctca ccaccgatgc ggtgatcgcc gccgccgacc tcgacaccgc cctgcgcacg
     2341 gccacgtcct tcaccttcga ccgcaccgac tccgacggct gcatgtcgac caacgacacc
     2401 gtgatcgtgc tggccagcgg ggcgtccggc gtcagtgccg acccggaaga gttcaccgag
     2461 gcgctcaccg gggtgtgtca ttccctggcg atgcaactgc tggccgactc cgagggctcc
     2521 gcacacgaca tcgaggtcac ggtgcacggc gccgcgtccg agtcggacgc gctcgcgatg
     2581 gcccgggcga tcgcgcgcag caacctgttc aagtgtgcgg tgttcggtaa cgaccccaac
     2641 tggggacggg tgctggccgc ggcgggcacc gtccgggccc gcttcgatcc cgatcaggtg
     2701 gacgtgtcct tcaacgacgt cggtgtcttc gtcggcggtc aactgggcgc ggaccgatcc
     2761 gaggtcgacc tctcggaacg ccaggtggtg gtcgaggtca acctgcacgc cggcgacgcc
     2821 tcggccacga tctggaccaa cgatctcacc tacgactacg tcaaagagaa cgcggagtac
     2881 tccacatgag ccccaccacc cgcacccggg ccacggccga gggcgtgatc ggcaaggccg
     2941 agacgctcat cgaggcactg ccctggctcg agcgctacgc cggcagcatc gtggtggtca
     3001 agtacggcgg caacgcgatg gtgaacgacg aactcaagcg cgccttcgcc gatgacatcg
     3061 tgttcctgcg ccgctgcggt ctcaagccgg tcgtggtgca cggcggtggc ccgcagatct
     3121 cgtcgatgct gggtcggctc ggcatcgcct cggagttcaa gggcggcctg cgggtcacct
     3181 cgcccgaagc catggacgtg gtgcgcatgg tgctggtcgg acaggtcgga cgcgagttgg
     3241 tcggcctgct gaacgcgcac ggaccccttg ccgttggcct gtccggcgag gacgccggcc
     3301 tgttcaccgc gaccaagcga ggcgtcacca tcgacggcga gcaggtcgac ctgggcctgg
     3361 ttggtgaggt gaccaccgtg cgtcccgaga cggtgatcga cctgatcgag ggcggacgca
     3421 ttccggtgat cgccaccgtg gcgcccgacg aggacggtca cgtgcacaac gtgaacgccg
     3481 acaccgcagc cggggcgctg gcggtcgccc tgggcgcgga acggtttctg gtgctcaccg
     3541 acgtcgaggg cttgtacgcc gactggccgg ccagcgaaga ggtgatcgca cgcatcacca
     3601 ccgcggagtt ggctgagctg ttgcccagcc tcgaggcggg catgattccc aagatggagg
     3661 cctgcctgca ggctgtcgag ggcggcgtgc ggcgtgccac catcatcgac ggacgggtgc
     3721 cgcacagcct gctgctcgag atcttcacct ccgaggggct gggcaccatg gtggtggccc
     3781 ctgacgacac ggcgacgggg gtgctcgcat gagcagcgcc tcgaacggga gcgcaagcgg
     3841 gcagccgcca ctcgcccagt cttcgtcgag cccggcacag ctgaaccagt cctcaaccgg
     3901 ccagtcctca accggccagt ccccaaccgg acagtcccca ctcggccagt cccaaaccca
     3961 gtcccacctc ggccagtcgc caaccggtca gtcccacctc acacagcgct accagcacgc
     4021 cgtgatgaac accttcgggc cacccaagcg cgtcttcgtc cgcggcgaag gcgtgcacct
     4081 gtgggacgcc gacggcaacc gttacctcga cctgctcgcc gggctggcgg tcaactcgct
     4141 ggggcacgcg cacccgcagg tgatcggtgc catctcggcg cagctctcca ccctcggtca
     4201 cgtgtcgaac ttcttcgcca cacccaccca gatcgcactc gccgagcgat tgtgcggctt
     4261 cgtcaccctc aatgaccccg gtcgcgaggc ccgggtgttc ttcaccaact cgggcaccga
     4321 ggccaacgag gcgggcttca agatcacccg gcgcaccgga cgcacccggc tgatcgccat
     4381 gcagggctcc tttcacggac gcagcctcgg cgccctcgcc ctgaccagca accccgccta
     4441 ccgcgaaccg ttcgagcccc tgcccggcga ggtgacgttc gtgccctacg gcgacgcgga
     4501 cgcgttggcg caggcgctgg acgacaccgt cgccgcggtg atcctggagc cgatccaggg
     4561 cgagaacggc gtcgtcgagc cgccgtcgga ctatctggcg gcagtccgcg cactgaccac
     4621 gaatgccggc gccctgctgt ggatcgacga ggtgcagacc ggtatcggac gctgcggcga
     4681 gtggctggcc agcgcccact ccggcgtcac cggcgacatc gtcaccttcg ccaagggcct
     4741 gggcaacggc ttcccggtcg
//
LOCUS       DAOSUG010000208         4756 bp    DNA     linear   ENV 10-JUN-2024
DEFINITION  MAG TPA_asm: Micropruina sp. isolate AMR_MDS_2195
            NODE_7800_length_4756_cov_5.819400, whole genome shotgun sequence.
ACCESSION   DAOSUG010000208 DAOSUG010000000
VERSION     DAOSUG010000208.1
DBLINK      BioProject: PRJNA925477
            BioSample: SAMN32864262
            Sequence Read Archive: SRR4236673
KEYWORDS    WGS; ENV; Metagenome Assembled Genome; MAG; Third Party Data; TPA;
            TPA:assembly.
SOURCE      Micropruina sp. (wastewater metagenome)
  ORGANISM  Micropruina sp.
            Bacteria; Bacillati; Actinomycetota; Actinomycetes;
            Propionibacteriales; Nocardioidaceae; Micropruina.
REFERENCE   1  (bases 1 to 4756)
  AUTHORS   Abdulkadir,N., Saraiva,J.P., Zhang,J., Stolte,S., Gillor,O.,
            Harms,H. and Rocha,U.
  TITLE     Genome-centric analyses of 165 metagenomes show that mobile genetic
            elements are crucial for the transmission of antimicrobial
            resistance genes to pathogens in activated sludge and wastewater
  JOURNAL   Microbiol Spectr 12 (3), e0291823 (2024)
   PUBMED   38289113
REFERENCE   2  (bases 1 to 4756)
  AUTHORS   Abdulkadir,N.
  TITLE     Direct Submission
  JOURNAL   Submitted (24-MAR-2023) Environmental Microbiology, Helmholtz
            Center for Environmental Research -UFZ, Permoserstr 15, Leipzig
            04318, Germany
COMMENT     The annotation was added by the NCBI Prokaryotic Genome Annotation
            Pipeline (PGAP). Information about PGAP can be found here:
            https://www.ncbi.nlm.nih.gov/genome/annotation_prok/
            
            ##Genome-Assembly-Data-START##
            Assembly Method        :: SPAdes v. 3.11.1
            Genome Representation  :: Full
            Expected Final Version :: Yes
            Genome Coverage        :: Not Applicable
            Sequencing Technology  :: Illumina MiSeq
            ##Genome-Assembly-Data-END##
            
            ##Genome-Annotation-Data-START##
            Annotation Provider               :: NCBI
            Annotation Date                   :: 05/04/2023 04:57:10
            Annotation Pipeline               :: NCBI Prokaryotic Genome
                                                 Annotation Pipeline (PGAP)
            Annotation Method                 :: Best-placed reference protein
                                                 set; GeneMarkS-2+
            Annotation Software revision      :: 6.5
            Features Annotated                :: Gene; CDS; rRNA; tRNA; ncRNA
            Genes (total)                     :: 2,776
            CDSs (total)                      :: 2,750
            Genes (coding)                    :: 2,714
            CDSs (with protein)               :: 2,714
            Genes (RNA)                       :: 26
            tRNAs                             :: 24
            ncRNAs                            :: 2
            Pseudo Genes (total)              :: 36
            CDSs (without protein)            :: 36
            Pseudo Genes (ambiguous residues) :: 0 of 36
            Pseudo Genes (frameshifted)       :: 2 of 36
            Pseudo Genes (incomplete)         :: 32 of 36
            Pseudo Genes (internal stop)      :: 2 of 36
            ##Genome-Annotation-Data-END##
FEATURES             Location/Qualifiers
     source          1..4756
                     /organism="Micropruina sp."
                     /mol_type="genomic DNA"
                     /submitter_seqid="NODE_7800_length_4756_cov_5.819400"
                     /isolate="AMR_MDS_2195"
                     /isolation_source="Activated sludge of municipal
                     wastewater treatment plants"
                     /db_xref="taxon:2737536"
                     /environmental_sample
                     /geo_loc_name="Canada"
                     /lat_lon="49.25 N 123.1 W"
                     /collection_date="2015-06-16"
                     /metagenome_source="wastewater metagenome"
                     /note="metagenomic"
     gene            complement(32..1285)
                     /locus_tag="PLF56_07820"
     CDS             complement(32..1285)
                     /locus_tag="PLF56_07820"
                     /inference="COORDINATES: protein motif:HMM:NF019310.2"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_function: GO:0022857 - transmembrane transporter
                     activity [Evidence IEA];
                     GO_process: GO:0055085 - transmembrane transport [Evidence
                     IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="MFS transporter"
                     /protein_id="HOQ53506.1"
                     /translation="MPPHVPYPVLLRTMGGSFTALGIIGRLPASILPLALLLYAQSQL
                     GSFALAGLATAALSLGGAAGAVFVGHLADTRGHRLIGVGATLVQTLAMAGFLFTCRPD
                     APLAVPLGCAALIGFANPQVGGMARARWSQAATQRADRTPFTSAAMAWEGAVDEVSFV
                     VGPVIATTLAAVAPALPVIIAMALAWIGQVGFALHRTALPPAHEGRLRHEHSPEHIDW
                     TMLVLLGVVTGAVGLLFGSTQTSLAAFFTLRDEQAVTGLVYGTMAVGSATMGLLTNRL
                     PARFSFSWRIVLFGLGSAVVSPLLVFAWDAPSMAVACFLVGVMVGPILVTAYALAERI
                     SPPERLSTLMAVLSTIGVVGVAAGAALAGQLVEVYTPQTAAWLATVGALLACVAGLIV
                     TRRLSAGSRSGPRGGSQREGRPDVA"
     gene            1567..2463
                     /locus_tag="PLF56_07825"
     CDS             1567..2463
                     /locus_tag="PLF56_07825"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_012922508.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="transglutaminase-like domain-containing protein"
                     /protein_id="HOQ53507.1"
                     /translation="MTTDVTNPQSFARHTAYSDPGVHVALVGALDPQLDALCATSRNL
                     VAHYRAELTDLPPERQGEIDSRWVEAILDLDQSRHPADLMSPRPLADRVAGCCRDHSL
                     LLASFLRTHGVPARTVVGFAGYFAPPFHHDHVVTEWYDGVRWVRADPELQAAGFDFDV
                     RDLPTGPGAPFETAAEVWLQYRDGALDADQYGVDPAIGPPLRGRDFIAGYVVFQVAHR
                     YGDELLLWDDWFTAPSDDEVDRLAALLVAADRGGSQSERALHDWYRSDAQLHPGRQIT
                     QHSPYGTPPVHVNLVTRSVLPD"
     gene            complement(2636..3082)
                     /gene="rplI"
                     /locus_tag="PLF56_07830"
     CDS             complement(2636..3082)
                     /gene="rplI"
                     /locus_tag="PLF56_07830"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_016454999.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_component: GO:0000311 - plastid large ribosomal subunit
                     [Evidence IEA];
                     GO_component: GO:0022625 - cytosolic large ribosomal
                     subunit [Evidence IEA];
                     GO_function: GO:0003735 - structural constituent of
                     ribosome [Evidence IEA];
                     GO_process: GO:0006412 - translation [Evidence IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="50S ribosomal protein L9"
                     /protein_id="HOQ53508.1"
                     /translation="MKLILTASVDHLGIPGEIVEVKDGYGRNYLLPRGFAIKWTKGAE
                     KQIDGIKRARDAREIRNVEHAREIRTQLEELTVTLPANAGDNGKLFGAVTAADIVLAV
                     KKAGGPAVDKRAVEIAKPIKTVGTHTVGLKLTSGVVAHINVNVAAA"
     gene            complement(3100..3336)
                     /gene="rpsR"
                     /locus_tag="PLF56_07835"
     CDS             complement(3100..3336)
                     /gene="rpsR"
                     /locus_tag="PLF56_07835"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_006503798.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_component: GO:0000314 - organellar small ribosomal
                     subunit [Evidence IEA];
                     GO_component: GO:0022627 - cytosolic small ribosomal
                     subunit [Evidence IEA];
                     GO_function: GO:0003735 - structural constituent of
                     ribosome [Evidence IEA];
                     GO_process: GO:0006412 - translation [Evidence IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="30S ribosomal protein S18"
                     /protein_id="HOQ53509.1"
                     /translation="MAQQRKPVNKKKIAPAKSVRIGHIDYKDTATLRKFISERGKIRA
                     RRVTGLSVQEQRKVAIAVKNARELALLPYASTAR"
     gene            complement(3420..3959)
                     /locus_tag="PLF56_07840"
     CDS             complement(3420..3959)
                     /locus_tag="PLF56_07840"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_002516468.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="single-stranded DNA-binding protein"
                     /protein_id="HOQ53510.1"
                     /translation="MAGETNITLVGNLTADPELRFTPSGAAVANFTVASTPRTFDRQT
                     SEWKDGDAMFLNCSVWRQAAENVAESLTKGMRVVVTGRLKSRSYETREGERRTVFEVE
                     VDEIGPSLRYASAKVTRNSGGGNYSGGGNSGGYSGGGGNSGGGNAGGNDAWSSGGGGN
                     NRSSGNDPWAQAQSEEPPF"
     gene            complement(4024..4314)
                     /gene="rpsF"
                     /locus_tag="PLF56_07845"
     CDS             complement(4024..4314)
                     /gene="rpsF"
                     /locus_tag="PLF56_07845"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_013866129.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_component: GO:0000314 - organellar small ribosomal
                     subunit [Evidence IEA];
                     GO_component: GO:0022627 - cytosolic small ribosomal
                     subunit [Evidence IEA];
                     GO_function: GO:0003735 - structural constituent of
                     ribosome [Evidence IEA];
                     GO_process: GO:0006412 - translation [Evidence IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="30S ribosomal protein S6"
                     /protein_id="HOQ53511.1"
                     /translation="MRQYEVMVIIDPDVDDRQVNGVLEKPLAGLTKAGGKVDNLDIWG
                     RRRLAYDIRKKSEGIYAVINITAEPADVKELDRQFTLNEQIMRTKVIRPDTR"
BASE COUNT          745 a         1611 c         1617 g          783 t
ORIGIN      
        1 gccgtacctc cggatcgctc accggttcac cctatgcgac gtccggacgc ccctcgcgtt
       61 gcgacccccc gcgcggcccg gatcgggagc cggccgacaa cctgcgcgtc acgatcagcc
      121 ctgccacgca cgccagcaat gcgccgacgg tggcgagcca cgccgcggtc tgtggggtgt
      181 atacctccac gagttgcccc gcgagtgccg ccccggcagc gaccccgacc acgccgatcg
      241 tggacagcac ggccatcagg gtcgacaacc gctcgggcgg cgaaatacgt tcggccaacg
      301 cgtacgccgt gaccagaatc ggcccgacca tgaccccgac caagaagcag gcgaccgcca
      361 tgctgggggc gtcccaggcg aacaccagca ggggcgacac aaccgcgctg cccaacccga
      421 acagcacgat tcgccagctg aacgagaagc gggccggcaa ccgattggtg agcaagccca
      481 tcgtggcgct gccgaccgcc atcgtgccgt agaccaggcc ggtgaccgcc tgctcgtcgc
      541 gcagcgtgaa gaacgcggca aggctggtct gcgtgctgcc gaacagcagc ccgaccgctc
      601 ccgtgaccac gccgagcagc accagcatcg tccagtcgat gtgctcgggc gagtgctcat
      661 ggcgaagccg tccctcgtgc gcaggaggca gcgcagtccg atgcagggcg aagccgacct
      721 gcccgatcca cgccagcgcc atggcgatga tcaccggcag cgcgggcgcg acggcggcca
      781 gggtggtcgc gatcaccgga cccaccacga acgacacctc gtcgaccgct ccctcccagg
      841 ccatggcggc cgaggtgaac ggcgtgcggt ccgcgcgttg cgtggccgcc tgtgaccagc
      901 gggcgcgtgc catgccgccc acctgcgggt tggcgaagcc gatcaaagcc gcacagccga
      961 gcggcacggc caggggcgcg tccggacggc acgtgaacag gaacccggcc atcgccagag
     1021 tctggacgag cgtcgccccg accccgatca gccggtgacc acgggtgtcg gccaggtgac
     1081 cgacgaagac cgcgcccgcc gcgccaccga ggctcagcgc cgcggtggcc aggccggcga
     1141 gcgcgaacga tccgagctgc gactgggcgt agagcagcag ggccagcggc agaatgctgg
     1201 cgggcaggcg tccgatgatg ccgagggccg tgaacgagcc gcccatggtg cgcagcagca
     1261 ccggatacgg cacgtgcggc ggcatggggc tcctttccga tcgctcgaca gcgttctgtg
     1321 cgtgacggtt tatgcggcga gcgtcgcgcg atgtcatcgg ggttaccttg ccacgcccga
     1381 tcccgtcgag ggcccacctc aaccgttcgg tcacggtgga gacgccgacg tcctgatccg
     1441 gctgcgcggc cacgtcgcgg ccacgtccgc gcggtggctc cgtcggccgg cgttgcacgc
     1501 gccccgcaac cgttcacgat ccgctcactt gacagtcaca ccgggccggg cgtcatggtg
     1561 tggggcatga ccaccgatgt cacgaacccc cagtcgttcg ctcggcacac cgcctattcc
     1621 gatccgggtg tgcacgtggc gttggtgggg gcgctcgatc cgcagctgga cgcgttgtgc
     1681 gcaacgagcc gcaatttggt ggcgcattac cgggccgagc tgaccgacct gcccccggag
     1741 aggcagggcg agatcgacag ccgttgggtg gaggcgatcc tcgacctcga ccagtcccgg
     1801 catcccgcag atctgatgag ccctcgtccg ctcgccgatc gtgtcgccgg ttgctgccgc
     1861 gaccattcgc tgctgctggc cagtttcttg cgcacccacg gggtgccggc gcgcacggtg
     1921 gtggggttcg cggggtactt cgcaccgccc tttcaccacg atcacgtggt caccgagtgg
     1981 tacgacggcg tccgttgggt gcgcgccgac cccgagttgc aggccgccgg gttcgacttc
     2041 gacgtgcgcg acctgccgac cgggccgggc gcacccttcg agaccgccgc cgaggtgtgg
     2101 ctgcagtacc gcgacggcgc cctggacgcc gatcagtacg gcgtggaccc ggccatcggc
     2161 cccccgttgc gcgggcggga cttcatcgcc ggctatgtgg tctttcaggt cgcccaccgc
     2221 tatggcgacg agctgttgct gtgggacgac tggttcacgg cgccgtccga tgatgaggtg
     2281 gatcgactgg ccgcactact ggtggcggcc gaccgaggcg gctctcagtc tgaacgcgcc
     2341 ctgcacgact ggtaccgcag cgacgcgcag ctacacccgg gccggcagat cacgcagcac
     2401 tcgccctacg gcactccacc ggtgcacgtg aacctggtca cccggtcggt gctgcccgac
     2461 tgagcacgaa tgccgttgtg gcgggctcag ccgggcgcac cggtccaagc gatggagaaa
     2521 gagccgacgt cgaggtcgaa cccagcccgt gatggtcgcg gccccgcgat cgggcatgac
     2581 gaaggggcgg ggtctgtcga ccccgcccct tcgcttactt ggctgaagaa ccgactcagg
     2641 cggcggcgac gttcacgttg atgtgagcga cgacaccact ggtgagcttg aggcccaccg
     2701 tgtgcgtgcc gacggtcttg atcggcttgg cgatctcgac ggcccgcttg tcgacggcgg
     2761 gcccgccggc cttcttgacg gcgagcacga tgtcggccgc ggtgacggcg ccgaacagct
     2821 tgccgttgtc accggcgttg gccggcagcg tcaccgtgag ctcttcgagc tgggtgcgga
     2881 tctcgcgggc atgctcgacg ttgcgaatct cacgagcgtc gcgcgcccgc ttgatgccgt
     2941 cgatctgctt ctcggcgccc ttggtccact tgatggcgaa accgcggggc aacaggtagt
     3001 tgcggccgta gccgtctttg acctcgacga tctcgcccgg aatgcccagg tggtcgacgg
     3061 aggcggtcag aatgagcttc atgcgttcgt ctacccttct cagcgagcgg tcgaggcgta
     3121 cggcagcagg gccagctcac gcgcgttctt gacggcgatc gcgaccttgc gctgctcttg
     3181 gacggacaga cccgtgaccc gccgagcgcg gatcttgccc cgctcgctga tgaacttgcg
     3241 cagggtggcg gtgtctttgt aatcgatgtg gccgatgcgc acggactttg ccggcgcgat
     3301 cttcttcttg ttcacaggct tgcgctgctg tgccattgtg gtgctccctt tcagtgagcc
     3361 cggccgaggc cggaatgacg tgactgtgtt gcgtgcggac gatcaacgcc cgcgggtgat
     3421 cagaacggcg gctcttcgct ctgggcctgc gcccacggat cgttgccgga cgagcggttg
     3481 ttgccgccac ccccggacga ccaggcgtcg ttaccgccgg cattgccgcc gccggagtta
     3541 cccccgccgc cggaatagcc gcccgagttg ccaccaccgg aatagttgcc gccgccggag
     3601 ttgcgggtga ccttggctga ggcgtaccgc agggacgggc cgatctcgtc gacctcgacc
     3661 tcgaagacgg tgcgacgttc gccctcacgg gtctcgtacg agcgcgactt caaccgacca
     3721 gtcaccacga cccgcatgcc cttggtgagc gattcggcga cgttctcggc cgcctgtcgc
     3781 cacaccgagc agttgaggaa catggcgtcg ccgtctttcc attcgctggt ctgacggtcg
     3841 aacgtgcgcg gcgtggacgc aacggtgaag ttggccaccg ccgcaccgga cggggtgaag
     3901 cgcagctcgg gatcggcggt caggttgccg acgagcgtga tgttggtttc gcctgccatg
     3961 agtctgtctt tcgatcgttg tgatgagaga cccggagtct gtcagccggc gccgacagtt
     4021 tttctagcgg gtgtcggggc ggatgacctt ggtgcgcatg atctgctcgt tgagcgtgaa
     4081 ctgacgatcc agttctttca catcggcagg ctctgcggtg atgttgatga cggcgtagat
     4141 gccttcggac ttcttgcgaa tgtcgtaggc gaggcgacgc cggccccaga tgtccaggtt
     4201 gtcgaccttg ccaccggcct tggtcaggcc tgcgagcggc ttttcaagaa cgccgttcac
     4261 ctgacggtcg tccacgtcgg gatcgatgat caccatgact tcgtattgac gcatacgcga
     4321 actccacctc ctctggtctc gagcggccat gagcatttcc catggcagga gggcgtgtgc
     4381 acggtgccag gaggcaccaa gggtcaactc tagcgtccgg acaccagagc cgacgaatcg
     4441 gagcgttgac tcatcgaaga tacccgcgtg ggatctgatg gctggagttc agccccctcg
     4501 gccgaaccct caccaagcgg gccggcgtcc ggcagacccg gtcatcgaca cgaccctgtg
     4561 atcgttaccg gcagccgagc gctgcggtgc agagtcgcgt ggcgcgtggc gcgtggcgcg
     4621 tggcgcgtgg cgcgtggcgc gcaaacctgc accacattcg agggggcgtc cgcctcggtg
     4681 aacgtcggag ggacgcgaat ctggtgcagt tctgcacggc tccgtccggc caaaggaacc
     4741 tctcgagcga aaacgg
//
LOCUS       DAOSUG010000209         4752 bp    DNA     linear   ENV 10-JUN-2024
DEFINITION  MAG TPA_asm: Micropruina sp. isolate AMR_MDS_2195
            NODE_7806_length_4752_cov_3.938258, whole genome shotgun sequence.
ACCESSION   DAOSUG010000209 DAOSUG010000000
VERSION     DAOSUG010000209.1
DBLINK      BioProject: PRJNA925477
            BioSample: SAMN32864262
            Sequence Read Archive: SRR4236673
KEYWORDS    WGS; ENV; Metagenome Assembled Genome; MAG; Third Party Data; TPA;
            TPA:assembly.
SOURCE      Micropruina sp. (wastewater metagenome)
  ORGANISM  Micropruina sp.
            Bacteria; Bacillati; Actinomycetota; Actinomycetes;
            Propionibacteriales; Nocardioidaceae; Micropruina.
REFERENCE   1  (bases 1 to 4752)
  AUTHORS   Abdulkadir,N., Saraiva,J.P., Zhang,J., Stolte,S., Gillor,O.,
            Harms,H. and Rocha,U.
  TITLE     Genome-centric analyses of 165 metagenomes show that mobile genetic
            elements are crucial for the transmission of antimicrobial
            resistance genes to pathogens in activated sludge and wastewater
  JOURNAL   Microbiol Spectr 12 (3), e0291823 (2024)
   PUBMED   38289113
REFERENCE   2  (bases 1 to 4752)
  AUTHORS   Abdulkadir,N.
  TITLE     Direct Submission
  JOURNAL   Submitted (24-MAR-2023) Environmental Microbiology, Helmholtz
            Center for Environmental Research -UFZ, Permoserstr 15, Leipzig
            04318, Germany
COMMENT     The annotation was added by the NCBI Prokaryotic Genome Annotation
            Pipeline (PGAP). Information about PGAP can be found here:
            https://www.ncbi.nlm.nih.gov/genome/annotation_prok/
            
            ##Genome-Assembly-Data-START##
            Assembly Method        :: SPAdes v. 3.11.1
            Genome Representation  :: Full
            Expected Final Version :: Yes
            Genome Coverage        :: Not Applicable
            Sequencing Technology  :: Illumina MiSeq
            ##Genome-Assembly-Data-END##
            
            ##Genome-Annotation-Data-START##
            Annotation Provider               :: NCBI
            Annotation Date                   :: 05/04/2023 04:57:10
            Annotation Pipeline               :: NCBI Prokaryotic Genome
                                                 Annotation Pipeline (PGAP)
            Annotation Method                 :: Best-placed reference protein
                                                 set; GeneMarkS-2+
            Annotation Software revision      :: 6.5
            Features Annotated                :: Gene; CDS; rRNA; tRNA; ncRNA
            Genes (total)                     :: 2,776
            CDSs (total)                      :: 2,750
            Genes (coding)                    :: 2,714
            CDSs (with protein)               :: 2,714
            Genes (RNA)                       :: 26
            tRNAs                             :: 24
            ncRNAs                            :: 2
            Pseudo Genes (total)              :: 36
            CDSs (without protein)            :: 36
            Pseudo Genes (ambiguous residues) :: 0 of 36
            Pseudo Genes (frameshifted)       :: 2 of 36
            Pseudo Genes (incomplete)         :: 32 of 36
            Pseudo Genes (internal stop)      :: 2 of 36
            ##Genome-Annotation-Data-END##
FEATURES             Location/Qualifiers
     source          1..4752
                     /organism="Micropruina sp."
                     /mol_type="genomic DNA"
                     /submitter_seqid="NODE_7806_length_4752_cov_3.938258"
                     /isolate="AMR_MDS_2195"
                     /isolation_source="Activated sludge of municipal
                     wastewater treatment plants"
                     /db_xref="taxon:2737536"
                     /environmental_sample
                     /geo_loc_name="Canada"
                     /lat_lon="49.25 N 123.1 W"
                     /collection_date="2015-06-16"
                     /metagenome_source="wastewater metagenome"
                     /note="metagenomic"
     gene            complement(<1..642)
                     /locus_tag="PLF56_07850"
     CDS             complement(<1..642)
                     /locus_tag="PLF56_07850"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_013864339.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_function: GO:0022857 - transmembrane transporter
                     activity [Evidence IEA];
                     GO_process: GO:0055085 - transmembrane transport [Evidence
                     IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="MFS transporter"
                     /protein_id="HOQ53512.1"
                     /translation="MKLDRSAVLVAVSVVIGGLAVIFDSTIISLALHTLADTLAVPVS
                     TIQWVSTGYLLALGVAIPVVGWAQACIGGKRLWLIALAAFGLASIACSLAWDAPSLIA
                     FRVAQGLAGGLMMPLMATLAMQQVVGGEGFGRLMAMVSLPAALGPILAPTLGGLILHW
                     LDWRWLFWVNVPFCVAGLVMAARFIPADPVPERRPRLDWAGLLLRWLHVITAIA"
     gene            722..1315
                     /locus_tag="PLF56_07855"
     CDS             722..1315
                     /locus_tag="PLF56_07855"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_013864340.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_function: GO:0003677 - DNA binding [Evidence IEA];
                     GO_process: GO:0006355 - regulation of DNA-templated
                     transcription [Evidence IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="TetR/AcrR family transcriptional regulator"
                     /protein_id="HOQ53513.1"
                     /translation="MDLESTPQRRRGSRLEADILDAAWQLLGTVGFDNLTFEAVAQAA
                     GTSRSVLYRRWPDREALVFAAIDRNFWSARPPLPDTGTLRDDLIELLRLANVARAPLV
                     GVMSALMGSYFSPGGMTFAALRERMLGDRIHSVDLLLQRAVERGEADPTRLTARVRAV
                     PFDLFRHDLLMTLSPLADDDIVAIVDEVFLPLVRPAT"
     gene            1374..2282
                     /locus_tag="PLF56_07860"
     CDS             1374..2282
                     /locus_tag="PLF56_07860"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_008359024.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="SDR family oxidoreductase"
                     /protein_id="HOQ53514.1"
                     /translation="MSDLTAIDNAAAELPSEKDGGFPAQEQQPPGLTSEMTPRPDHGE
                     EGWVGRDRLHGLKALITGGDSGIGRAVAIAFAREGADVALSYLSAEQSDAEDTAGWVE
                     KAGRKAVLLPGDLTDEATARAVVDDAAEQLGGLDVLVNNAGFQWARREGGLADVATDE
                     LDRVFKTNLYAMFWVTQQALKYLGKGSSIINVSSIQAYEPSTALIDYASTKAAINNFT
                     VNLAGELGPRGIRVNAVAPGPIWTPLQPATKPGEAMTGFGGDTPLGRPGQPSELAGAF
                     VFLASPLEASYVSATVLGVTGGKAVF"
     gene            2326..2484
                     /locus_tag="PLF56_07865"
     CDS             2326..2484
                     /locus_tag="PLF56_07865"
                     /inference="COORDINATES: ab initio
                     prediction:GeneMarkS-2+"
                     /note="Derived by automated computational analysis using
                     gene prediction method: GeneMarkS-2+."
                     /codon_start=1
                     /transl_table=11
                     /product="hypothetical protein"
                     /protein_id="HOQ53515.1"
                     /translation="MPKGMVEEGRAADQHVKESEQDEGRSTDRAQEIAARSVNKQRSK
                     EGRTKKSG"
     gene            2599..4275
                     /locus_tag="PLF56_07870"
     CDS             2599..4275
                     /locus_tag="PLF56_07870"
                     /inference="COORDINATES: protein motif:HMM:NF024491.2"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="phospholipase D-like domain-containing protein"
                     /protein_id="HOQ53516.1"
                     /translation="MNTVSFALTAAEFARRGLLGFCVERAVEGGPFIWMQGFKVFRSL
                     VPDPRPTDQVSTYSHPIQALVWDDFTLRPDTTYTYRFHPMRGTPAKPRRNRPIAITIH
                     TEPLHAGVSDVVFNRGVASSQAYMRRFGGRSPDDQPTPQLRQQALDWLSRDLDDVLLE
                     LIGGAGPGDALHGAFYEFNYPPVLDALVAAAVRGVDVKLVVDFKTTASGPREANRAAI
                     AAAGFPAGVIIAREARVSAIAHNKFLVLSHGGAPAAVWTGSTNLTTGGVHGQSNVGHL
                     VRDASASTAYHAYFKLLAGDPGGRSSDTSAARRTKNAAFKAQVAALSPAPADRAGVAA
                     GITPVFSPRPDLGPLHTYAQLIGAGATSLACATFAFGIAQEIQDAAAGTPDGPLRFFL
                     LETRGKATVRLDASRNVYQAWGSELDHPLGQWVAETTTRELGLNRHVAFIHDKFLLCD
                     PLGPDPVVVTGSANFSRASTLENDENMIIIRGDTRVADIYFTEFNRLFFHYYFRSVVQ
                     QVSARAGAPESDAHALDLVENDTWTLKYAPGTLRAKRVQKLAEMADPLDA"
     gene            4363..>4752
                     /locus_tag="PLF56_07875"
     CDS             4363..>4752
                     /locus_tag="PLF56_07875"
                     /inference="COORDINATES: protein motif:HMM:NF026760.2"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="alpha/beta-hydrolase N-terminal
                     domain-containing protein"
                     /protein_id="HOQ53517.1"
                     /translation="MNAVSSVGLVLGLCAFLVSFAPSLLPRRWLEQGLIGGAVAMIGY
                     LVGLLLEETADALGRLIGLDVQVNWRMPGGYLVLTLLGAALVVLGWWWSVREHRETAR
                     LVDMPLQPVWHDIASTGVALGFLAVLVL"
BASE COUNT          779 a         1645 c         1633 g          695 t
ORIGIN      
        1 ggcgatcgcg gtaatgacgt ggagccaccg caacagcagg ccggcccagt cgaggcgcgg
       61 acggcgctcg ggcaccggat cggcgggaat gaaccgggcc gccatcacca ggccggcgac
      121 gcagaacggc acgttcaccc agaacaacca gcgccagtcg agccagtgca ggatcagccc
      181 gccgagggtc ggggccagga tcggcccgag tgccgcgggc aggctgacca tcgccatcag
      241 cctgccgaag ccctcgccac ccaccacctg ttgcatggcc agggtcgcca tcagcggcat
      301 catcaggccg ccggccaggc cttgcgcgac gcggaacgcg atcaggctgg gggcgtccca
      361 ggcgaggctg caggcgatcg aggccaaccc gaaggcggcc aacgcgatga gccagagacg
      421 cttgccgccg atgcacgcct gcgcccagcc gaccaccgga atggccaccc cgagagcgag
      481 cagatagccg gtgctgaccc actggatggt gctcaccggc accgcgaggg tatcggccag
      541 agtgtgcagc gcgaggctga tgatcgtgct gtcgaagatc accgccaggc cgccgatgac
      601 caccgagacg gcaacgagta ccgccgatcg gtcgagcttc accgctggtt ccgccaccat
      661 tccccctaag agacgtggat gtatctaaaa gaacgatacg cgcagaggtt aagatacgca
      721 aatggatctt gagtcgaccc cgcaacgacg tcgcggcagc cgcctcgagg ccgacatcct
      781 tgacgccgcg tggcaactgc tcggcacggt cggcttcgac aacctgacct tcgaggcggt
      841 ggcgcaggcg gccggaacca gccggtctgt gctgtatcgc cgctggcccg atcgcgaggc
      901 gctggtcttc gcagcaatcg atcgcaactt ctggagcgca cgtccgccgc tgccggacac
      961 gggcaccctt cgggacgacc tgatcgagct attgcgcctg gccaacgtcg cgcgcgcccc
     1021 cctggtgggg gtgatgagcg ccctgatggg cagctacttc tcgcccggcg gcatgacctt
     1081 cgccgccttg cgcgagcgca tgctgggcga tcgcatccac agcgtcgacc tactgctgca
     1141 gcgcgcggtc gagcggggcg aggccgaccc gacccgcctg actgctcgcg tgcgggccgt
     1201 gccgttcgac ctgtttcggc acgacctgct gatgaccctg tcccccctcg ccgacgacga
     1261 catcgtcgcg atcgtcgacg aggtgttcct gccgttggtg cgtccggcga cgtgaccgcc
     1321 gcccacccct gggtgggtag agggttgctg gttcgcccgt gcaggctgga gcgatgagtg
     1381 atctcaccgc catcgacaac gccgcggccg aactgccttc cgagaaggac gggggctttc
     1441 ccgcccagga gcagcaacca cccggcctga ccagcgagat gaccccgaga cccgatcacg
     1501 gcgaggaagg ctgggtcggc cgcgaccggt tgcacggtct caaagcgctg atcaccggcg
     1561 gcgactccgg catcggacgg gcggtggcaa tcgccttcgc ccgtgagggt gcagatgtcg
     1621 ccctgagcta cctgtccgcc gagcagtcgg acgccgaaga caccgcgggc tgggtcgaga
     1681 aagccggtcg taaggccgtg ctcctgcctg gcgacctgac cgacgaggcc accgcgcgcg
     1741 ccgtggtcga cgacgcggcc gaacagctgg gcgggctcga cgtcttggtg aacaacgccg
     1801 ggttccagtg ggcacggcgc gagggcggtc tggccgacgt ggccaccgac gagctggatc
     1861 gcgtgttcaa gacgaacctg tacgccatgt tctgggtcac ccagcaggcg ctgaagtacc
     1921 tcggcaaggg gtccagcatc atcaacgtca gctctattca ggcctacgag ccgtccacgg
     1981 ccctgatcga ctacgcctcg accaaggcgg ccatcaacaa cttcaccgtc aatctggccg
     2041 gcgagctcgg cccgcgcggc atcagggtca atgccgtggc gcccggcccg atctggacgc
     2101 cgctgcagcc ggccaccaag cccggcgagg cgatgaccgg cttcggcggt gacacgccgc
     2161 tgggcaggcc ggggcagccg tccgaactgg ccggggcgtt cgtgttcctg gcgagcccgc
     2221 tcgaggcgag ctacgtgtcg gccaccgtgc tgggcgtcac cggcggcaag gcggtgttct
     2281 gagcaggtcg ggcacgcacc cgtccaccag cgaaggaggc gacagatgcc gaagggcatg
     2341 gtcgaagaag gacgagcggc agaccagcac gtcaaagaga gcgagcagga cgaaggtcgt
     2401 tccaccgatc gcgcccaaga gatcgcggct cgcagcgtga acaagcagcg cagtaaagaa
     2461 gggcgcacga agaagtccgg ctgaggtcgc ggtcgggcaa cggcggccag gtatcaattc
     2521 gcgtccgcgg ggctactttg aagcatgcgc taccggtcac gccctgtgcg tggcatcagg
     2581 gcctacgcgg tgtgcggcgt gaacaccgtg tcgttcgccc tgaccgccgc cgagttcgcc
     2641 cgcagagggc tgttggggtt ctgcgtagaa cgtgcggtcg agggtgggcc gttcatctgg
     2701 atgcaggggt tcaaggtgtt ccgctcgctg gtgcccgacc ccaggccgac cgaccaggtg
     2761 agcacctaca gccacccgat tcaggccctg gtctgggacg acttcacgct tcggcccgac
     2821 accacctaca cctaccgctt tcacccgatg cggggaacgc cggccaagcc gaggcgcaac
     2881 cggccgatcg ccatcacgat tcacaccgag ccgctgcacg ccggcgtgag cgacgtggtg
     2941 ttcaaccggg gcgtggcctc cagccaggcc tacatgcgcc ggttcggtgg caggtcgccc
     3001 gacgaccagc ccaccccgca gttgcgccag caggcgctcg actggctcag ccgcgacttg
     3061 gacgacgtgc tgctcgagct gatcggcggc gccggcccgg gtgatgcgct gcacggcgcc
     3121 ttctacgagt tcaactaccc tccggtgctg gacgctctgg tggcggcggc agtccggggg
     3181 gtggacgtga agctggtggt cgacttcaag accaccgcga gcgggccgcg cgaggccaac
     3241 cgggcagcca tcgcggccgc cggctttccg gccggggtga tcatcgcccg cgaggcccgg
     3301 gtctcggcta tcgcgcacaa caagttcctg gtgctcagcc acggcggcgc cccggctgcg
     3361 gtctggaccg gctcaaccaa cctgaccacg ggcggcgtgc acgggcagtc caatgtcggc
     3421 cacctggtgc gcgacgcgtc ggcttcgacc gcctaccacg cctacttcaa gctgctggcc
     3481 ggcgacccgg gcggacggtc gtccgacact tcggcggcca ggcgcacaaa gaacgccgcg
     3541 ttcaaggcac aggtggcagc gctcagcccg gcaccggccg accgcgccgg ggtggcggcc
     3601 ggcatcaccc cggtgttcag ccctcggccc gacctcggcc cgctccacac ctacgcccag
     3661 ctgatcgggg ccggcgccac gtcgttggcc tgcgccacgt tcgcgttcgg catcgcgcag
     3721 gagatccagg acgccgcggc cggcacgccc gacggcccac tgcggttctt cctgctcgaa
     3781 acccgcggca aggccaccgt ccgcctggac gcgtcccgca acgtgtacca ggcgtggggc
     3841 tccgagctcg accacccgct cggccagtgg gtggccgaga ccaccacccg cgagttgggc
     3901 ctgaaccggc acgtggcgtt cattcacgac aagttcctgc tctgcgatcc gctcggcccc
     3961 gatccggtgg tggtcaccgg ctcggcgaac ttctcgcggg cgtccacgct cgagaacgac
     4021 gagaacatga tcatcatccg cggggacacg cgggtggccg acatctactt caccgagttc
     4081 aaccggctgt tttttcacta ctacttccgc tcggtggtgc agcaggtgtc ggcccgggct
     4141 ggggcgcccg agtcggacgc ccacgccctc gacctggtcg agaacgacac ctggacgctc
     4201 aagtacgcac ccggcacgct gcgcgcgaag cgggtgcaga agctggccga gatggccgac
     4261 ccactcgacg cctgaaccgc accgagccaa gcacgggaac gccggcgagc aatgcgatcg
     4321 gcctgggcct aggctgaacc cggtcgaaca aggggcgatg agatgaacgc ggtatcgagt
     4381 gtcggcctgg tgctcggcct gtgcgcgttc ctggtctctt tcgcccccag cctgctgccg
     4441 cggcgctggc tcgaacaggg gctgatcggc ggggcggtgg cgatgatcgg ctacctggtc
     4501 ggccttctgc tcgaagagac cgcggacgcc ctgggccggc tgatcggact cgacgtacag
     4561 gtcaactggc gcatgcccgg cggctacctg gtgctcacgc tgctgggggc cgcgctggtg
     4621 gtgctcggct ggtggtggtc ggtgcgtgag caccgcgaga ccgcccggct ggtcgacatg
     4681 ccgctgcagc cggtgtggca cgacatcgca tccactggtg tggcattggg gttcttggcg
     4741 gtgctcgttc tg
//
LOCUS       DAOSUG010000210         4751 bp    DNA     linear   ENV 10-JUN-2024
DEFINITION  MAG TPA_asm: Micropruina sp. isolate AMR_MDS_2195
            NODE_7807_length_4751_cov_3.264480, whole genome shotgun sequence.
ACCESSION   DAOSUG010000210 DAOSUG010000000
VERSION     DAOSUG010000210.1
DBLINK      BioProject: PRJNA925477
            BioSample: SAMN32864262
            Sequence Read Archive: SRR4236673
KEYWORDS    WGS; ENV; Metagenome Assembled Genome; MAG; Third Party Data; TPA;
            TPA:assembly.
SOURCE      Micropruina sp. (wastewater metagenome)
  ORGANISM  Micropruina sp.
            Bacteria; Bacillati; Actinomycetota; Actinomycetes;
            Propionibacteriales; Nocardioidaceae; Micropruina.
REFERENCE   1  (bases 1 to 4751)
  AUTHORS   Abdulkadir,N., Saraiva,J.P., Zhang,J., Stolte,S., Gillor,O.,
            Harms,H. and Rocha,U.
  TITLE     Genome-centric analyses of 165 metagenomes show that mobile genetic
            elements are crucial for the transmission of antimicrobial
            resistance genes to pathogens in activated sludge and wastewater
  JOURNAL   Microbiol Spectr 12 (3), e0291823 (2024)
   PUBMED   38289113
REFERENCE   2  (bases 1 to 4751)
  AUTHORS   Abdulkadir,N.
  TITLE     Direct Submission
  JOURNAL   Submitted (24-MAR-2023) Environmental Microbiology, Helmholtz
            Center for Environmental Research -UFZ, Permoserstr 15, Leipzig
            04318, Germany
COMMENT     The annotation was added by the NCBI Prokaryotic Genome Annotation
            Pipeline (PGAP). Information about PGAP can be found here:
            https://www.ncbi.nlm.nih.gov/genome/annotation_prok/
            
            ##Genome-Assembly-Data-START##
            Assembly Method        :: SPAdes v. 3.11.1
            Genome Representation  :: Full
            Expected Final Version :: Yes
            Genome Coverage        :: Not Applicable
            Sequencing Technology  :: Illumina MiSeq
            ##Genome-Assembly-Data-END##
            
            ##Genome-Annotation-Data-START##
            Annotation Provider               :: NCBI
            Annotation Date                   :: 05/04/2023 04:57:10
            Annotation Pipeline               :: NCBI Prokaryotic Genome
                                                 Annotation Pipeline (PGAP)
            Annotation Method                 :: Best-placed reference protein
                                                 set; GeneMarkS-2+
            Annotation Software revision      :: 6.5
            Features Annotated                :: Gene; CDS; rRNA; tRNA; ncRNA
            Genes (total)                     :: 2,776
            CDSs (total)                      :: 2,750
            Genes (coding)                    :: 2,714
            CDSs (with protein)               :: 2,714
            Genes (RNA)                       :: 26
            tRNAs                             :: 24
            ncRNAs                            :: 2
            Pseudo Genes (total)              :: 36
            CDSs (without protein)            :: 36
            Pseudo Genes (ambiguous residues) :: 0 of 36
            Pseudo Genes (frameshifted)       :: 2 of 36
            Pseudo Genes (incomplete)         :: 32 of 36
            Pseudo Genes (internal stop)      :: 2 of 36
            ##Genome-Annotation-Data-END##
FEATURES             Location/Qualifiers
     source          1..4751
                     /organism="Micropruina sp."
                     /mol_type="genomic DNA"
                     /submitter_seqid="NODE_7807_length_4751_cov_3.264480"
                     /isolate="AMR_MDS_2195"
                     /isolation_source="Activated sludge of municipal
                     wastewater treatment plants"
                     /db_xref="taxon:2737536"
                     /environmental_sample
                     /geo_loc_name="Canada"
                     /lat_lon="49.25 N 123.1 W"
                     /collection_date="2015-06-16"
                     /metagenome_source="wastewater metagenome"
                     /note="metagenomic"
     gene            complement(157..1908)
                     /locus_tag="PLF56_07880"
     CDS             complement(157..1908)
                     /locus_tag="PLF56_07880"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_017935354.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_component: GO:0016020 - membrane [Evidence IEA];
                     GO_function: GO:0015103 - inorganic anion transmembrane
                     transporter activity [Evidence IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="SulP family inorganic anion transporter"
                     /protein_id="HOQ53518.1"
                     /translation="MDAAAAPRWARYAPGLHVLLHYDRSWLRGDLLAGVTVAAYLIPQ
                     VMAYAEIARLPPIAGLWAILAPLAVYALLGSSRQLSIGPESTTALMTAAGLGALLGAA
                     GGTERWSEVAALMAIAVGVICLVGRLFRLGFLANLLSRPVLVGYLAGIAVLMITSQLG
                     KVTGVVIEGDAPGAQWWSLVTRLGTVHLPTLALAIAVGVALVACRQWLPTWPGPLICM
                     LAAAGSVFLFGLDRLGVRVIGAVPQGLPAPRVPLLDDLSVLSLLPYALGIAVVGYSDN
                     VLTARAFAVKRKEPVDSNQELLALGAANVVNGFTNGFPVSSSGSRTVIGDAVGSRSQL
                     YSLVCLALVIAALLVLGPVLAWFPAAALGALVIFAAIRLIDVAELRRIARFRRSELLL
                     ALATTLGVLWLGVLGGIGLAIGLSVLDLIRRIASPHDGVLGYVPGMAGMHDVDDYPIV
                     VQVPGLVVYRYDSPLFFANAEDFLTRALLAVEQADPRCDWFVLNAEANVEVDLTSVDI
                     LETLRAALAGRGIRFALARVKFELRQQLQAAGFVQQVGEEFIFATLPTAVAAYADWYQ
                     RTRGERPPGVPAQRGQG"
     gene            2034..3707
                     /locus_tag="PLF56_07885"
     CDS             2034..3707
                     /locus_tag="PLF56_07885"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_015071856.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="glycoside hydrolase family 13 protein"
                     /protein_id="HOQ53519.1"
                     /translation="MSVPADHETVISATDALWWRQAVVYQIYPRSFADSNADGIGDFA
                     GIVSRAPYLAALGIDAVWLNPFYPSDLADGGYDVIDHRDVDARIGTLADFDALVEVLH
                     AHGIRLLIDIVPNHTSDHHRWFREALDAPPGSSARARYLFRDGTGTDGAQPPTDWRSL
                     FGGSTWQQVPDGQWYLHTFAVEQPDLNWANDEVRDDFLTTLRFWADRGVDGFRVDAAH
                     MLAKDLSDPLPSQDELDLMPRDGNHPLLDRDELQQIYAEWRAVFNAYDPPRTAVAEAA
                     VHASRVPRYASPQTLGQSFFFDLQLADYDPASFHRIIDECLRVAVTSGSSVTWVLNNH
                     DTIRHTSRYGTPFPGYGPDGEPVRKHGGDWLMAGGDPVLLDAARGSRRARAATMLLLA
                     LPGSAYLYQGEELGLHEVAELPDAVRQDPSFLRNRAVEVGRDGCRVPLPWTRTGPSFG
                     FGAGGAHLPQPLWFGHYAVEAQQDDPSSTLALYRRALSLRRTLQGAESLQWLPTGRDD
                     VLSFSRPGGWRVVSNFGARPCPLPDGEVLLTSNPLSDHLPGETTVWLRQ"
     gene            complement(3757..4245)
                     /gene="mnhG"
                     /locus_tag="PLF56_07890"
     CDS             complement(3757..4245)
                     /gene="mnhG"
                     /locus_tag="PLF56_07890"
                     /inference="COORDINATES: protein motif:HMM:NF009314.0"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_function: GO:0051139 - metal cation:proton antiporter
                     activity [Evidence IEA];
                     GO_process: GO:0098662 - inorganic cation transmembrane
                     transport [Evidence IEA];
                     GO_process: GO:1902600 - proton transmembrane transport
                     [Evidence IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="monovalent cation/H(+) antiporter subunit G"
                     /protein_id="HOQ53520.1"
                     /translation="MNLHEVMALIGAFLLAAGGFFCFGGALGLVRFPDILARMHSATK
                     PQVFGLMCALLGVGFSIPDPKMWMLLAVVMLLQILTAPVAGHLIGRVAYRSGEWEPED
                     LVVDELAADLRDAGFHRNEDFRPERATDRPTSRTLPPDQTGRTLAPDENGRTLPEAGS
                     SE"
     gene            complement(4242..4559)
                     /locus_tag="PLF56_07895"
     CDS             complement(4242..4559)
                     /locus_tag="PLF56_07895"
                     /inference="COORDINATES: protein motif:HMM:NF015993.2"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_component: GO:0016020 - membrane [Evidence IEA];
                     GO_function: GO:0015075 - monoatomic ion transmembrane
                     transporter activity [Evidence IEA];
                     GO_process: GO:0034220 - monoatomic ion transmembrane
                     transport [Evidence IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="monovalent cation/H+ antiporter complex subunit
                     F"
                     /protein_id="HOQ53521.1"
                     /translation="MIALLTQALIWLAMALVTGAAALTLWRLTKGPSTLDRIVSADVL
                     LSIVLISICLTMVISGYTSAMPILLVLSMVAFTGGVSVARMLAPGAPDRQRYRREKQE
                     DEL"
     gene            complement(4556..>4751)
                     /locus_tag="PLF56_07900"
     CDS             complement(4556..>4751)
                     /locus_tag="PLF56_07900"
                     /inference="COORDINATES: protein motif:HMM:NF014012.2"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_component: GO:0016020 - membrane [Evidence IEA];
                     GO_function: GO:0008324 - monoatomic cation transmembrane
                     transporter activity [Evidence IEA];
                     GO_process: GO:0006812 - monoatomic cation transport
                     [Evidence IEA]"
                     /codon_start=2
                     /transl_table=11
                     /product="Na+/H+ antiporter subunit E"
                     /protein_id="HOQ53522.1"
                     /translation="IVPGSIVIDARRSNRVLYLHLVDVVGDQGVEEARAAALDVERRV
                     LEAMAGTSEIAAIRRQEERP"
BASE COUNT          768 a         1761 c         1579 g          643 t
ORIGIN      
        1 tgctgcatgg cgcggccggg cggcgcttca cgcgctggat cggcatcggg ctggtgatcg
       61 tcggcatcgc cctattggtg gacctgttcc tgattccgac ctacacccgc gacgccaact
      121 accgtctcac ggggtttcgg tactagggcg cgtctcctaa ccctggccgc gctgcgccgg
      181 aaccccgggc ggccgctcgc cgcgtgtgcg ctggtaccag tcggcgtacg cggccaccgc
      241 cgtgggcagg gtggcgaaga tgaactcctc acccacctgc tgcacgaagc cggccgcctg
      301 cagctgctga cgcagttcga acttgacccg ggccagcgca aacctgatgc cgcgccccgc
      361 cagcgccgca cgcagcgttt cgaggatgtc cacgctggtc aggtcgacct cgacgttggc
      421 ctcggcattc agcacgaacc agtcgcaccg ggggtcggcc tgctcgaccg cgagcaacgc
      481 ccgggtcaag aagtcctccg cgttggcgaa gaacagcggc gagtcgtacc ggtagaccac
      541 cagaccgggc acctggacga cgatcgggta gtcgtccacg tcgtgcatgc ccgccatacc
      601 cggcacgtag ccgagcacgc cgtcgtgcgg gctggcgatc cggcggatca ggtcgagcac
      661 cgacagtccg atcgccaggc cgataccgcc cagcacgccg agccacagca cgccgagtgt
      721 tgtcgccagg gccagcagca actcgctgcg cctgaaccgg gcgatccggc gaagctcggc
      781 cacgtcgatc agccggatcg ccgcgaagat caccagcgcc ccgagcgcgg cggccggaaa
      841 ccaggccagc accgggccga gcaccagcag tgccgcgatc accagcgcca aacagaccag
      901 tgagtacagc tggctgcggc tgccgaccgc gtcaccgatc accgtgcgac tgcccgacga
      961 actgaccgga aacccgttgg tgaagccgtt gaccacgttg gccgcgccca gcgcgagcag
     1021 ctcctggttg ctgtccaccg gctctttgcg tttgacggcg aaagccctgg ccgtgagcac
     1081 attgtcgctg tagccgacaa cggcgatgcc cagcgcgtac ggcagcaggg acagcaccga
     1141 caggtcgtcc agcagcggga cgcgcggcgc cggcagcccc tgcggcaccg caccgatcac
     1201 ccgcaccccc agccggtcga gaccgaagag gaacacgctg ccggcagcgg cgagcatgca
     1261 gatcagcggg ccgggccagg tcggcagcca ttgtcgacag gccaccaatg ccacgccgac
     1321 cgcgatcgcc agcgccagcg tcggcaggtg gacggtgccg agccgcgtga ccagcgacca
     1381 ccactgcgca cccggcgcgt ccccttcgat gaccacgccg gtgaccttgc cgagctggct
     1441 ggtgatcatc agcaccgcga tgccggccag atacccgacc agcaccgggc gcgacagcag
     1501 gttggccagg aatcccagcc tgaacagccg gccgaccagg cagatcaccc cgacggcgat
     1561 cgccatcagt gccgccacct cggaccagcg ctccgtgccg cccgcggccc ccagcagcgc
     1621 gcccagcccc gccgcggtca tcagcgccgt ggtcgattcg ggcccgatcg acagttgccg
     1681 ggacgagccc agcaaggcat agacggcaag tggcgccagg atcgcccaca acccggcgat
     1741 cggcggcagc cgggcgatct cggcgtaggc catcacctgc gggatgaggt aggccgccac
     1801 ggtgactccg gccagcaggt cgccgcgcaa ccaggaccgg tcgtagtgca gcagcacgtg
     1861 cagacccggt gcgtaccgcg cccaacgcgg cgccgcagca gcgtccatgc cggaacggta
     1921 ctggtcgccc gcgcgcggtg ccacgcccac gccccacccg ccggataagg tgagccccac
     1981 cccgtccgcg acattggtgc cgagacgcgg cgcccccact cgaaaggacc gcgatgtctg
     2041 tgccggccga tcacgagacg gtcatctcgg ccacggacgc cctgtggtgg cggcaggcgg
     2101 tggtgtacca gatctacccg cgcagcttcg ccgacagcaa cgccgacggc atcggcgact
     2161 tcgccggcat cgtctcgcgg gcgccctatc tggccgcgct gggcatcgac gcggtgtggc
     2221 tgaacccgtt ctacccctct gatctggccg acggcggcta cgacgtgatc gaccaccgcg
     2281 acgtcgatgc ccggatcggc accctcgccg acttcgacgc cctggtcgag gtgctgcacg
     2341 cccacggcat ccggctgctg atcgacatcg tgccgaacca cacctccgac catcaccgct
     2401 ggttccgcga ggccctcgac gccccgcccg ggtcgagcgc ccgcgcacgc tacctgttcc
     2461 gcgacggcac cggcaccgac ggtgcccagc cgcccaccga ttggcgctcg ctgttcggcg
     2521 ggtcgacctg gcagcaggtg ccggacggcc agtggtacct gcacaccttc gccgtcgagc
     2581 agccggacct gaactgggcc aacgacgagg tgcgcgatga ctttctgacc accctgcggt
     2641 tctgggccga ccggggtgtc gacggcttcc gggtggacgc cgcccacatg ctcgccaaag
     2701 acctgagcga cccactaccc tcccaggacg aactggacct gatgccgcgc gacggcaacc
     2761 atccgctgct cgaccgcgac gaactgcagc agatctatgc cgagtggcgg gccgtgttca
     2821 acgcctacga cccgccccgt acggcggtcg ccgaggcggc cgtgcatgcc tcgcgggtgc
     2881 cgcgttacgc cagcccgcag acgctgggcc agtcgttctt cttcgatctg caactcgccg
     2941 actacgaccc ggccagtttt caccgcatca tcgacgagtg cctgagggtc gcggtgacgt
     3001 ccggatcgtc ggtcacctgg gtgctgaaca accacgacac gatccggcac accagccgct
     3061 acggcacgcc gttccccggc tacgggcccg acggcgaacc cgtccgcaag cacggcggcg
     3121 actggctgat ggccggcggt gaccccgtgc tgctggacgc ggcgcgcggc tcgcgacggg
     3181 cgcgcgcggc caccatgctg ctgctcgcgc tgcccggctc ggcctacctg taccagggcg
     3241 aggagctcgg cctgcacgag gttgccgagc tgccggacgc cgtccggcag gacccgtcct
     3301 tcttgcgcaa ccgcgccgtc gaggtcggac gcgacggctg ccgcgtgccg ctgccgtgga
     3361 cccgcaccgg gccgtcgttc gggttcggcg ccggcggtgc gcacctgcca cagccgctct
     3421 ggttcgggca ctacgcggtc gaggcgcagc aggacgaccc gtcgtccaca ctggccctgt
     3481 accgccgcgc actgagcctg cggcgcacgc tgcagggtgc cgagtcgctg cagtggctgc
     3541 ccaccggccg cgacgacgtg ctcagcttca gccgcccggg cgggtggcgg gtggtgagca
     3601 acttcggtgc gcggccgtgc ccgctgcccg acggcgaggt gctgctcacc tccaaccccc
     3661 tgagcgacca tctgccgggg gaaacgaccg tgtggctgag gcagtagccg aatccgttcc
     3721 cgacagtcgc agcgcactgc ggtcgtggct gcggtactac tcgctcgagc cggcttccgg
     3781 cagggtgcgg ccgttctcgt cgggcgccag ggtgcggccg gtctggtcgg gcggcagggt
     3841 gcggctggtc ggacggtccg tcgcccgctc ggggcgaaag tcctcgttgc gatgaaagcc
     3901 ggcgtcgcgc aggtccgcgg cgagttcgtc cacgaccagg tcttccggct cccactcacc
     3961 ggagcggtac gcgacccgtc cgatcagatg accggcgacc ggcgcggtca gaatctgcag
     4021 cagcatcacc actgccagca gcatccacat cttggggtcg ggaatcgaga agcccacccc
     4081 cagcagggcg cacatcaggc cgaacacctg cggcttggtc gccgagtgca tgcgggccag
     4141 aatgtcggga aagcgcacca ggccgagcgc cccaccgaag cagaagaagc ccccagcggc
     4201 aagcagaaat gcgccgatca gtgccatcac ctcgtggagg ttcatagctc atcctcctgc
     4261 ttctcgcggc ggtaacgctg ccgatccggc gccccgggcg ccagcatgcg ggccacgctg
     4321 acgccgccgg tgaaggccac catcgacagc accagcagaa tcggcatggc gctggtgtag
     4381 ccgctgatca ccatggtcag gcagatgctg atcagcacga tcgacaacag cacgtcggcg
     4441 ctgacgatgc gatcgagcgt tgagggacct ttcgtgagcc gccacagggt cagcgcggcg
     4501 gccccggtca ccagtgccat tgccagccag atcagggcct gggtgagcag ggcgatcatg
     4561 gccgctcctc ctgccgtcga atggcggcga tctcggacgt gccggccatg gcctccagca
     4621 cgcggcgctc cacgtcgagc gccgccgcgc gggcctcttc gaccccctgg tcgccgacca
     4681 cgtcgaccag gtgcaggtac agcacccggt tggaccgccg ggcgtcgatc acgatcgagc
     4741 cgggcacgat c
//
LOCUS       DAOSUG010000211         4741 bp    DNA     linear   ENV 10-JUN-2024
DEFINITION  MAG TPA_asm: Micropruina sp. isolate AMR_MDS_2195
            NODE_7820_length_4741_cov_4.215109, whole genome shotgun sequence.
ACCESSION   DAOSUG010000211 DAOSUG010000000
VERSION     DAOSUG010000211.1
DBLINK      BioProject: PRJNA925477
            BioSample: SAMN32864262
            Sequence Read Archive: SRR4236673
KEYWORDS    WGS; ENV; Metagenome Assembled Genome; MAG; Third Party Data; TPA;
            TPA:assembly.
SOURCE      Micropruina sp. (wastewater metagenome)
  ORGANISM  Micropruina sp.
            Bacteria; Bacillati; Actinomycetota; Actinomycetes;
            Propionibacteriales; Nocardioidaceae; Micropruina.
REFERENCE   1  (bases 1 to 4741)
  AUTHORS   Abdulkadir,N., Saraiva,J.P., Zhang,J., Stolte,S., Gillor,O.,
            Harms,H. and Rocha,U.
  TITLE     Genome-centric analyses of 165 metagenomes show that mobile genetic
            elements are crucial for the transmission of antimicrobial
            resistance genes to pathogens in activated sludge and wastewater
  JOURNAL   Microbiol Spectr 12 (3), e0291823 (2024)
   PUBMED   38289113
REFERENCE   2  (bases 1 to 4741)
  AUTHORS   Abdulkadir,N.
  TITLE     Direct Submission
  JOURNAL   Submitted (24-MAR-2023) Environmental Microbiology, Helmholtz
            Center for Environmental Research -UFZ, Permoserstr 15, Leipzig
            04318, Germany
COMMENT     The annotation was added by the NCBI Prokaryotic Genome Annotation
            Pipeline (PGAP). Information about PGAP can be found here:
            https://www.ncbi.nlm.nih.gov/genome/annotation_prok/
            
            ##Genome-Assembly-Data-START##
            Assembly Method        :: SPAdes v. 3.11.1
            Genome Representation  :: Full
            Expected Final Version :: Yes
            Genome Coverage        :: Not Applicable
            Sequencing Technology  :: Illumina MiSeq
            ##Genome-Assembly-Data-END##
            
            ##Genome-Annotation-Data-START##
            Annotation Provider               :: NCBI
            Annotation Date                   :: 05/04/2023 04:57:10
            Annotation Pipeline               :: NCBI Prokaryotic Genome
                                                 Annotation Pipeline (PGAP)
            Annotation Method                 :: Best-placed reference protein
                                                 set; GeneMarkS-2+
            Annotation Software revision      :: 6.5
            Features Annotated                :: Gene; CDS; rRNA; tRNA; ncRNA
            Genes (total)                     :: 2,776
            CDSs (total)                      :: 2,750
            Genes (coding)                    :: 2,714
            CDSs (with protein)               :: 2,714
            Genes (RNA)                       :: 26
            tRNAs                             :: 24
            ncRNAs                            :: 2
            Pseudo Genes (total)              :: 36
            CDSs (without protein)            :: 36
            Pseudo Genes (ambiguous residues) :: 0 of 36
            Pseudo Genes (frameshifted)       :: 2 of 36
            Pseudo Genes (incomplete)         :: 32 of 36
            Pseudo Genes (internal stop)      :: 2 of 36
            ##Genome-Annotation-Data-END##
FEATURES             Location/Qualifiers
     source          1..4741
                     /organism="Micropruina sp."
                     /mol_type="genomic DNA"
                     /submitter_seqid="NODE_7820_length_4741_cov_4.215109"
                     /isolate="AMR_MDS_2195"
                     /isolation_source="Activated sludge of municipal
                     wastewater treatment plants"
                     /db_xref="taxon:2737536"
                     /environmental_sample
                     /geo_loc_name="Canada"
                     /lat_lon="49.25 N 123.1 W"
                     /collection_date="2015-06-16"
                     /metagenome_source="wastewater metagenome"
                     /note="metagenomic"
     gene            complement(<1..1690)
                     /locus_tag="PLF56_07905"
     CDS             complement(<1..1690)
                     /locus_tag="PLF56_07905"
                     /inference="COORDINATES: ab initio
                     prediction:GeneMarkS-2+"
                     /note="Derived by automated computational analysis using
                     gene prediction method: GeneMarkS-2+."
                     /codon_start=1
                     /transl_table=11
                     /product="hypothetical protein"
                     /protein_id="HOQ53523.1"
                     /translation="MTAALNLAPLTAGALLADPRLAGELAPSNHPLALLPVRLETRYA
                     GGELLVRIYPDQVHVDAHDPRLSAAEQQAGQDFWRTQWRTGDNVARQQRAWKMLADRY
                     GAGRAGWVVRATRPSNPGERPGTEVGDDVAPRPAPAFPTLEVSDDRRTPVARLLPDRW
                     TVTAYAGGQVLAVATGAPIAADPAVGPDLAAPLVGDADDHQVAAFDQGMNWLVDFAAA
                     EEIGMAVRLPVTTPVDLLLATGVGRQDGAAGADRLGALLSAQRYSSGLGFLPPDTPTN
                     NSEAAGSGWSSTAAGAWSPSAAAPPPDSAGLAAAQALGLADPELFGSLPGAGDTGALL
                     TRAMARALWPATWGYWLTQFVGVDAATNDWGREFAGLFVRPEGPLPTLRVGRQPYGLL
                     PVTSLGRFAGDDRTTRLARVLGGLRDGAWRPAVDLAPRIGRSDVASDFVDVLRLEARS
                     DDLRLRRSMGARFADNLQRFLFRDLTDGGFWTAARDRSLPLARAAGLGLLPGALTLHE
                     PSDHPVTLPLVSAGDDVSFIGALLAADPDQLAGSTETPESVLAVLLRHGLLREHA"
     gene            complement(1687..4533)
                     /locus_tag="PLF56_07910"
     CDS             complement(1687..4533)
                     /locus_tag="PLF56_07910"
                     /inference="COORDINATES: ab initio
                     prediction:GeneMarkS-2+"
                     /note="Derived by automated computational analysis using
                     gene prediction method: GeneMarkS-2+."
                     /codon_start=1
                     /transl_table=11
                     /product="hypothetical protein"
                     /protein_id="HOQ53524.1"
                     /translation="MTEPSLLFLPWVRQGGSLALPPDDRATHPPSHVATTVGVSVNDG
                     PGASVPVQLLGPGDVTGVAPQQVIRSDPAPNAQNFESNYLALVEFDDPGLPWLFTPAS
                     AAGGTLRPWLCLVVVRQQPGVQLLPAGRGALPVLQIGAPARPDVELPDLADSWAWAHA
                     QVALDGAAGVAAVTAALPGDPARNLSRLVCGRLLAEQTTYLACVVPTFEAGRRAGLGD
                     DPGDAQGPAWTPAEGMGTVELPVYHHWRFATGPTGDFQSLAMAIRGRPVEDGFGLRPV
                     DLSTAGLGLGGTDDAQELLGGALLALDAPTKRWSDEALPARFATALRAVLNAADAASV
                     AEPLLAPPRYGSAYRSPAALSSGPGAWYEQLNLDPAARVAAALGTLVVQRQQETLVGA
                     AWDQSVDLSAGTRLVGLAGLGFAMAKSLHRRHIAPLSPQTGLFVLAPLRAALMSAPAT
                     TAGQSFGAQWAKAQLPGSTLGSAVRRATRVRGPAIRTAERDGTMPPLLDWIGSMVPGA
                     VLARGFEAPSGPLTFDAVGPTLPAPGPVSWAGLTAAAVTGAAQHPGFTIAATPAPTGP
                     RPFPLPGEVATGGLDGRVLGRTLRTADDPAGGSGRALRRPDDGGGPVPIRRRPGDGNT
                     PLTPPTPPTPVDPPHHPPRRDSAAAALFRAVAAKQLAGFAPARPDPGLLLGSVALESL
                     MTEAVALTAPTRTFAAAIGTLFSAPPAPATSESPLPDLRFTPYFDTPMAHSLADLGQH
                     WLLPGLAGVPADTALALRTNGAFVEAFMVGLNHELGRELLWREFPTRLTATFFDRFWD
                     AAVAPSEPPDIPQLDGWGERALGAPTVRPERFVLLLRSELMRRFPDAVVSATRPGANP
                     PETLLPVFRGSLDPDVTFFGFTIALADADEYTISIAEQPGAPRFGFEVGEAPDGVSHA
                     PAPDASAAQLAGRLRQLPARITIPITVLMREPQS"
     gene            complement(4530..>4741)
                     /locus_tag="PLF56_07915"
     CDS             complement(4530..>4741)
                     /locus_tag="PLF56_07915"
                     /inference="COORDINATES: ab initio
                     prediction:GeneMarkS-2+"
                     /note="Derived by automated computational analysis using
                     gene prediction method: GeneMarkS-2+."
                     /codon_start=3
                     /transl_table=11
                     /product="hypothetical protein"
                     /protein_id="HOQ53525.1"
                     /translation="EIVVGDDGVPVVQQQTQPAPRDQVLAGLRISASARALTRTSAAE
                     RFSGVARADAPRLATLGLARTAGVS"
BASE COUNT          615 a         1745 c         1865 g          516 t
ORIGIN      
        1 cggcatgctc gcgcagcagg ccgtgccgaa gcagcacggc cagcaccgac tcgggtgttt
       61 cggtggatcc ggccagctgg tcggggtcgg cggcgagcag cgcgccgatg aacgacacgt
      121 cgtcgccggc cgacaccagc ggcagcgtca ccggatggtc ggacggctcg tgcaacgtca
      181 gcgcgcccgg cagcagcccg agcccggcgg cccgggccag cggcagtgac cggtcgcggg
      241 cggcggtcca gaacccgccg tcggtgaggt cacggaacag gaagcgctgc aggttgtcgg
      301 cgaaccgggc acccatgctg cgccgcagcc gcaggtcgtc cgagcgtgcc tcgagccgca
      361 gcacgtcgac gaagtcggac gccacgtcgc tgcgaccgat ccgcggggcc aggtcgacgg
      421 ccggacgcca tgcgccgtcc cgcagcccgc ccagcacccg ggccagccgg gtggtgcggt
      481 cgtcgccggc gaagcggccg agcgaggtga ccggcagcag gccgtagggc tgccggccca
      541 cccgcagcgt cggcagtgga ccctcgggcc gcacgaacag cccggcgaac tcgcggcccc
      601 agtcgttggt cgccgcgtcg acgccgacga actgggtgag ccaatagccc caggtggccg
      661 gccacagcgc ccgggccatc gcccgggtga gcagcgcgcc ggtgtcgccg gcgcccggca
      721 ggctgccgaa cagttccggg tcggcgaggc cgagggcctg cgcggccgcc agaccggccg
      781 aatcgggcgg tggggcggcg gccgacggcg accaagcccc ggcggcggtg ctcgaccagc
      841 cggaacccgc cgcctcggag ttgttcgtcg gggtgtccgg cggcaggaaa cccagcccgg
      901 acgagtaccg ctgcgcgctc agcagcgccc cgagccggtc ggcgccggcc gcgccgtcct
      961 ggcggccgac gccggtggcc agcagcaggt cgaccggcgt ggtcaccggc agccggacgg
     1021 ccatgccgat ctcttcggcg gcggcgaagt cgaccagcca gttcatgccc tggtcgaacg
     1081 cggccacctg gtgatcgtcg gcgtcgccga ccaggggtgc ggccaggtcg gggccgaccg
     1141 cgggatcggc ggcgatcggc gctccggtgg ccaccgccag cacctgcccg ccggcgtagg
     1201 cggtcaccgt ccagcggtcc ggcagcaacc gggcgacggg ggtgcggcgg tcgtccgaga
     1261 cctcgagcgt ggggaaggcc ggcgcgggtc gcggggccac gtcgtcgccg acctcggtgc
     1321 ccggtcgctc accgggattg ctcggacggg tggcccgcac cacccagccg gcccggccgg
     1381 cgccgtaccg gtcggcgagc atcttccagg cccgttgctg cctggccacg ttgtcgccgg
     1441 tgcgccactg ggtgcgccag aagtcctgcc cggcctgctg ctcggccgcc gacagccgcg
     1501 gatcgtgcgc gtcgacgtgc acctggtcgg ggtagatgcg caccagcagc tcgccgcccg
     1561 catagcgggt ctccagccgc accggcagca gtgccagcgg gtggttcgag ggggccagtt
     1621 cgccggccag tcgcggatcg gccagtaggg cgcccgcggt cagcggggcg aggttcagcg
     1681 cagcggtcat gattgcggct cccgcatcag caccgtgatc ggaatggtga tccgggccgg
     1741 caactgacgc agccggccgg ccagttgggc ggcggacgcg tccggggccg gggcgtggct
     1801 gaccccgtcc ggcgcctcgc ccacctcgaa gccgaaccgt ggcgcgccgg gctgctcggc
     1861 gatgctgatc gtgtactcgt ccgcgtcggc cagggcaatg gtgaagccga agaacgtcac
     1921 gtcggggtcg agcgacccgc ggaacaccgg cagcagggtc tccggcgggt tcgcgcccgg
     1981 cctggtggcg ctcaccacgg cgtccgggaa gcgccgcatc agttcgctgc gcagcagcag
     2041 cacgaaccgc tccggacgca ccgtcggcgc acccagtgcg cgctcgcccc acccgtccag
     2101 ttgcggaatg tccggcggtt cggacggcgc caccgcggcg tcccagaacc ggtcgaagaa
     2161 ggtggcggtc agccgggtgg ggaactcgcg ccacagcagt tcgcggccga gctcgtggtt
     2221 gagccccacc atgaaggcct cgacgaaggc tccgttggtg cgcagcgcca gcgcagtgtc
     2281 ggccggtacg ccggccagtc cgggcagcag ccagtgctgg ccgagatcgg ccagcgagtg
     2341 ggccatcggg gtgtcgaagt agggcgtgaa gcgcaggtcg ggcagcggag actcggacgt
     2401 ggccggggcc ggcggcgcgc tgaacagggt gccgatggcg gccgcgaagg tgcgggtcgg
     2461 tgcggtcagc gccaccgctt cggtcatcaa cgattccagc gcgacgctgc cgagcagcag
     2521 cccggggtcg ggccgggccg gcgcgaaccc ggccagctgc ttggcggcca ccgcgcggaa
     2581 cagcgcagcc gcggccgagt cgcggcgcgg cggatggtgc ggcgggtcga ccggcgtcgg
     2641 cggggtgggc ggggtgagcg gggtgttgcc gtcgccgggc cggcggcgaa tcggcaccgg
     2701 cccgccgccg tcgtcggggc ggcgcaacgc ccgtccggac ccgcccgccg gatcgtcggc
     2761 agtgcgcaag gtccggccca gcactcggcc gtcgaggccg ccggtggcca cctctcccgg
     2821 cagcgggaag ggccgcggac cggtgggtgc cggggtggcc gcgatggtga aaccggggtg
     2881 ctgggccgcc ccggtgaccg cagcggcggt gaggcccgcc cacgacaccg gacccggcgc
     2941 gggcagcgtc ggcccgaccg cgtcgaaggt gagcggaccg ctgggcgcct cgaacccgcg
     3001 ggccagcacg gcaccgggca ccatgctgcc gatccagtcc agcagcgggg gcatggtgcc
     3061 gtcgcgctcg gcggtgcgga tcgcggggcc gcgcacccgg gtcgcccgcc ggacggccga
     3121 accgagcgtc gatccgggca gctgcgcctt cgcccactgg gcgccgaagc tctgcccggc
     3181 ggtggtcgcc ggcgccgaca tcagggccgc tcgcagcggg gccagcacga acagtccggt
     3241 ctgcggcgac agcggcgcga tgtggcggcg gtgcaggctc ttggccatcg cgaaccccag
     3301 tccggccagg ccgaccagcc gggtgccggc gctcaggtcg accgactggt cccacgcggc
     3361 gccgaccagg gtctcctgct ggcgctgcac gaccagcgtg cccagcgcgg cagcgacccg
     3421 ggccgccgga tcgaggttga gctgctcgta ccacgccccc ggcccggaac tcagcgcggc
     3481 gggtgagcgg tacgccgagc cgtaccgggg cggggccagc agcggttcgg cgaccgaagc
     3541 ggcgtccgcc gcgttcagca ccgcccgcag tgcggtggcg aaccgggccg gcagggcctc
     3601 gtccgaccag cgcttcgtcg gggcgtccag ggcgagcagg gcgccgccga gcagctcctg
     3661 ggcgtcgtcg gtgccgccca gcccgagccc cgcggtggac aagtcgaccg gacgcagccc
     3721 gaacccgtcc tcgaccggac gtccgcggat cgccatcgcc agcgactgga agtcgccggt
     3781 cggcccggtg gcgaagcgcc agtggtggta gaccggtaac tcgaccgtgc ccatgccctc
     3841 ggccggcgtc caggccgggc cctgcgcgtc gccggggtca tcgcccaggc cggcgcgccg
     3901 gccggcctcg aaggtcggca ccacgcacgc caggtaggtg gtctgctcgg cgagcagccg
     3961 gccgcacacc agccgggaca ggttgcgggc cgggtcgccg ggcagcgctg cggtgacggc
     4021 ggccaccccg gcggcgccgt ccagggcgac ctgggcgtgc gcccaggccc acgagtcggc
     4081 caggtcgggc aactccacgt ccggacgggc cggcgcaccg atctgcagca ccggcagcgc
     4141 gccccggccg gccggcagca gctggacgcc cggctgctgc cgcaccacca ccaggcacag
     4201 ccagggccgc agcgtgccgc cggcggccga ggcgggggtg aacagccacg gcaggcccgg
     4261 gtcgtcgaac tcgaccaggg ccaggtagtt cgactcgaag ttctgcgcgt tgggcgcggg
     4321 gtcggagcgg atcacctgct gcggcgccac cccggtgacg tcgccggggc cgagcagctg
     4381 caccggcacc gacgcgcccg gaccgtcgtt gacgctcacc ccgacggtgg tggccacgtg
     4441 gctgggcggg tgcgtggccc ggtcgtccgg cggcagcgcg agcgaaccac cctggcgcac
     4501 ccagggcagg aacagcaggc tgggttcggt catgacaccc ccgccgtccg ggccagcccg
     4561 agggtggcca gccggggagc gtcggcgcga gccacgccgc tgaaccgttc ggccgcggac
     4621 gtgcgggtca gcgcccgggc ggacgcgctg atccgcagcc cggccagcac ctggtcgcgc
     4681 ggtgccggct gggtctgctg ttgcaccacc ggcacgccgt cgtcgccgac cacgatctcg
     4741 g
//
LOCUS       DAOSUG010000212         4736 bp    DNA     linear   ENV 10-JUN-2024
DEFINITION  MAG TPA_asm: Micropruina sp. isolate AMR_MDS_2195
            NODE_7831_length_4736_cov_4.135868, whole genome shotgun sequence.
ACCESSION   DAOSUG010000212 DAOSUG010000000
VERSION     DAOSUG010000212.1
DBLINK      BioProject: PRJNA925477
            BioSample: SAMN32864262
            Sequence Read Archive: SRR4236673
KEYWORDS    WGS; ENV; Metagenome Assembled Genome; MAG; Third Party Data; TPA;
            TPA:assembly.
SOURCE      Micropruina sp. (wastewater metagenome)
  ORGANISM  Micropruina sp.
            Bacteria; Bacillati; Actinomycetota; Actinomycetes;
            Propionibacteriales; Nocardioidaceae; Micropruina.
REFERENCE   1  (bases 1 to 4736)
  AUTHORS   Abdulkadir,N., Saraiva,J.P., Zhang,J., Stolte,S., Gillor,O.,
            Harms,H. and Rocha,U.
  TITLE     Genome-centric analyses of 165 metagenomes show that mobile genetic
            elements are crucial for the transmission of antimicrobial
            resistance genes to pathogens in activated sludge and wastewater
  JOURNAL   Microbiol Spectr 12 (3), e0291823 (2024)
   PUBMED   38289113
REFERENCE   2  (bases 1 to 4736)
  AUTHORS   Abdulkadir,N.
  TITLE     Direct Submission
  JOURNAL   Submitted (24-MAR-2023) Environmental Microbiology, Helmholtz
            Center for Environmental Research -UFZ, Permoserstr 15, Leipzig
            04318, Germany
COMMENT     The annotation was added by the NCBI Prokaryotic Genome Annotation
            Pipeline (PGAP). Information about PGAP can be found here:
            https://www.ncbi.nlm.nih.gov/genome/annotation_prok/
            
            ##Genome-Assembly-Data-START##
            Assembly Method        :: SPAdes v. 3.11.1
            Genome Representation  :: Full
            Expected Final Version :: Yes
            Genome Coverage        :: Not Applicable
            Sequencing Technology  :: Illumina MiSeq
            ##Genome-Assembly-Data-END##
            
            ##Genome-Annotation-Data-START##
            Annotation Provider               :: NCBI
            Annotation Date                   :: 05/04/2023 04:57:10
            Annotation Pipeline               :: NCBI Prokaryotic Genome
                                                 Annotation Pipeline (PGAP)
            Annotation Method                 :: Best-placed reference protein
                                                 set; GeneMarkS-2+
            Annotation Software revision      :: 6.5
            Features Annotated                :: Gene; CDS; rRNA; tRNA; ncRNA
            Genes (total)                     :: 2,776
            CDSs (total)                      :: 2,750
            Genes (coding)                    :: 2,714
            CDSs (with protein)               :: 2,714
            Genes (RNA)                       :: 26
            tRNAs                             :: 24
            ncRNAs                            :: 2
            Pseudo Genes (total)              :: 36
            CDSs (without protein)            :: 36
            Pseudo Genes (ambiguous residues) :: 0 of 36
            Pseudo Genes (frameshifted)       :: 2 of 36
            Pseudo Genes (incomplete)         :: 32 of 36
            Pseudo Genes (internal stop)      :: 2 of 36
            ##Genome-Annotation-Data-END##
FEATURES             Location/Qualifiers
     source          1..4736
                     /organism="Micropruina sp."
                     /mol_type="genomic DNA"
                     /submitter_seqid="NODE_7831_length_4736_cov_4.135868"
                     /isolate="AMR_MDS_2195"
                     /isolation_source="Activated sludge of municipal
                     wastewater treatment plants"
                     /db_xref="taxon:2737536"
                     /environmental_sample
                     /geo_loc_name="Canada"
                     /lat_lon="49.25 N 123.1 W"
                     /collection_date="2015-06-16"
                     /metagenome_source="wastewater metagenome"
                     /note="metagenomic"
     gene            complement(<1..780)
                     /locus_tag="PLF56_07920"
     CDS             complement(<1..780)
                     /locus_tag="PLF56_07920"
                     /inference="COORDINATES: ab initio
                     prediction:GeneMarkS-2+"
                     /note="Derived by automated computational analysis using
                     gene prediction method: GeneMarkS-2+."
                     /codon_start=1
                     /transl_table=11
                     /product="conjugal transfer protein"
                     /protein_id="HOQ53526.1"
                     /translation="MIDAAYTAWQRDLASGLASILVAETHETVTTLNVRARADRIIDG
                     TVRPDREIPLHDDTAVSEGDLVITRHNDRRLRNGSSWVRNGNRWTVARVRSDGSITVR
                     PTGRRFGGAIVLPAVYVAEHLDLGYAITAHRAQGVTTDTAHVVATATTTRENFYVAMT
                     RGREGNYAYVAIDRPDDAHDVPHPSDDPDATARSVLYGVIQHVGAELSAHETIVAEQE
                     HRGGITQLAAEYETIAQAAQHDRWATLLAASGLTGEQVDAVL"
     gene            complement(777..1067)
                     /locus_tag="PLF56_07925"
     CDS             complement(777..1067)
                     /locus_tag="PLF56_07925"
                     /inference="COORDINATES: protein motif:HMM:NF024991.2"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="AAA family ATPase"
                     /protein_id="HOQ53527.1"
                     /translation="MAGSGTASLDRITDLAERAGAKVLLVGDWGQLQSVDAGGAFSTL
                     VGAWDDAPELLDIHRFTHAWEKTTSLQLRHGRSRAIDTLSTTAGSPGARRRR"
     gene            complement(1315..1893)
                     /locus_tag="PLF56_07930"
     CDS             complement(1315..1893)
                     /locus_tag="PLF56_07930"
                     /inference="COORDINATES: ab initio
                     prediction:GeneMarkS-2+"
                     /note="Derived by automated computational analysis using
                     gene prediction method: GeneMarkS-2+."
                     /codon_start=1
                     /transl_table=11
                     /product="DUF4190 domain-containing protein"
                     /protein_id="HOQ53528.1"
                     /translation="MSNPHNSPWAQGDQADQPRPQPTSGLELPPDDHPSSNYSNPVLD
                     RPGPVQQVGRPDSDGSVAGGWSEPGGWSEPQTFQALQPADERSAPVQFGQRLPYGYAA
                     APQPEHPNATLVLVLGIVGLFTNFMLLPFVSPIAWYLGAKARREMARHPGVYSDSGKL
                     TAGVTLGVIGTVFALLGVAFIVLLILLAIGIS"
     gene            complement(1952..2800)
                     /locus_tag="PLF56_07935"
     CDS             complement(1952..2800)
                     /locus_tag="PLF56_07935"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_017933195.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="A/G-specific adenine glycosylase"
                     /protein_id="HOQ53529.1"
                     /translation="MVASIVAWYGEHARDLPWRRPDTSPWAVLVSEFMLQQTPVRRVL
                     QPWQQWVSRWPTPAALAAEPVGEALRAWGRLGYPRRAQRLHAAATAITSEHEGRVPSD
                     PAVLRTLPGVGDYTAAAVASFAFGARQVVLDVNVRRVLARLEGGTEAPAAAPTQAERR
                     RAEAWLPDAGAARWSVAAMELGAQVCRAGDPLCSACPVQNDCRWLAAGRPPGPPRRRQ
                     LYEGTDRAARGHLLAHLRALPTDAAATEPRLLEGWPDRAQAVRALQSLVDDGLVRRDG
                     DDGYRL"
     gene            2931..3593
                     /locus_tag="PLF56_07940"
     CDS             2931..3593
                     /locus_tag="PLF56_07940"
                     /inference="COORDINATES: ab initio
                     prediction:GeneMarkS-2+"
                     /note="Derived by automated computational analysis using
                     gene prediction method: GeneMarkS-2+."
                     /codon_start=1
                     /transl_table=11
                     /product="hypothetical protein"
                     /protein_id="HOQ53530.1"
                     /translation="MSEVLTGKGPQPPSVYWIRRGLVALAAVIIIGLLVWAIVPKNAP
                     STATPAANSPTPAPSALDTTTSAGASPTASPSDGTTSPSPTTSAAAAAACDPIGVQLS
                     VEGFKSVKADAKQVFSVTVENNTALPCLMQITKDTFRLRVASGKDMIWSTAHCDTWLP
                     EVKQQTLKAGAAVEFKINWSLHRSAEGCKQTKDVLGAGTYVATAMYRDTATGKFPFTI
                     TK"
     gene            complement(3841..>4736)
                     /gene="disA"
                     /locus_tag="PLF56_07945"
     CDS             complement(3841..>4736)
                     /gene="disA"
                     /locus_tag="PLF56_07945"
                     /EC_number="2.7.7.85"
                     /inference="COORDINATES: protein motif:HMM:NF010009.0"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=3
                     /transl_table=11
                     /product="DNA integrity scanning diadenylate cyclase DisA"
                     /protein_id="HOQ53531.1"
                     /translation="FAIDVTFTGTALRELAKMDGAIILDDALDRILFAGVHLMPDAAV
                     ATSETGTRHRTADRVARQTGVPTVTVSASMSTISLFVDERRWVVQRPEQLLARANQAL
                     ATLVRFSDRLGEATARLSAAEVQDAATVRDIALVAQRLEMVRRLRLEVTGYAVELGTD
                     GRLVRLQLQELVSGLDELSTHLQRDYTDDTTTPQLARLAGLEADDLASPGLIAQACGL
                     GDDLSRRMQPQGHRQLAQIGRLAPPVADSLVAHFGTLQGLFGATTADLLGVDGVDYSI
                     ARTVREGLIRLAESAYTERLD"
BASE COUNT          715 a         1618 c         1688 g          715 t
ORIGIN      
        1 gagcacggca tcgacctgct cgccggtcag ccccgaggcg gcaaggaggg tggcccagcg
       61 gtcgtgttgg gcggcttgcg cgatcgtctc gtactcggcc gcgagctgcg tgatcccacc
      121 ccggtgctcc tgctcagcga cgatggtctc gtgcgctgac agctcggcgc caacgtgttg
      181 gatcacgccg tagagcactg accgggcggt ggcgtccggg tcgtcggagg ggtgtggtac
      241 gtcgtgggcg tcgtcgggcc ggtcaatggc gacgtaggcg tagttcccct cccggccccg
      301 cgtcatcgcg acgtagaagt tctccctggt cgtggtcgcc gtcgcaacaa catgggcggt
      361 atcggtggtg acgccctgag ccctatgcgc cgtgatcgcg tatcccaagt ccaaatgctc
      421 ggctacatac accgccggga gcacgatggc gccgccgaat cgtcgcccgg tcgggcgcac
      481 ggtgatcgac ccgtcggagc ggacgcgggc gacggtccag cggttgccgt tgcggaccca
      541 tgaactgccg ttgcggagcc tgcggtcgtt gtggcgggtg atgaccagat caccttcgga
      601 gacggcggtg tcgtcatgca gcggaatttc gcgatctggt cgaacggtgc cgtcgatgat
      661 ccgatccgcc cgcgcccgca cgttgagggt ggtcacggtc tcgtgggttt cggccaccag
      721 gatcgacgcc aacccagaag cgaggtcgcg ctgccaggcg gtgtaggcgg catcgatcat
      781 cgcctccgtc tcgcccccgg tgatccggcc gtggtcgata aggtgtcgat ggcccgggag
      841 cggccgtggc gcagctgcaa cgaggtggtc ttctcccagg cgtgggtgaa gcggtgaata
      901 tcgagcagct ccggggcgtc atcccaggca ccgacgagcg ttgagaacgc acccccggcg
      961 tcaacggact ggagttgccc ccagtcgccg accagcagca ccttcgcacc ggcccgctca
     1021 gccagatcgg tgatccggtc cagggacgca gtgcccgagc ccgccaaccc tcatcggcgc
     1081 ccttctggga atcgctgttg cgaggattcg gcgcatgaac ttgaggacgc ggagttgccg
     1141 cccggacgtc cgagctcagg agccttgcac cggcaacggg gggccggccc tagctgccct
     1201 agctggggtg aggacttcgc tttccgtcgg tcacttgtca ccccgggttt gcctcggcat
     1261 ctgggcgcgc gcagggatcc cggcgtgcgc cgggatgacg ggggttggga cgctctagct
     1321 gatgcctatg gcgagcagga tcagcagcac gatgaaggcg acgcccagca gcgcgaacac
     1381 ggtaccgatg acgccgaggg tgacacctgc ggtcagcttg ccgctgtcgc tgtacacgcc
     1441 cgggtgcctc gccatttctc ggcgggcctt ggcgccgagg taccaggcga tcgggctgac
     1501 gaacggcagc agcatgaagt tggtgaacaa gccgacgatg ccgagaacca gcaccagcgt
     1561 cgcgttgggg tgctcaggct gcggcgctgc tgcgtagccg tagggcagcc gttggccgaa
     1621 ctgaaccggt gccgatcgct cgtccgcggg ctgtagcgcc tggaaggtct gcggctcgga
     1681 ccagccaccc ggctcggacc agccgccggc cacggacccg tcggagtccg gccggccgac
     1741 ctgctggacg gggccaggcc ggtcgagcac cgggttgctg tagttgctgg acgggtggtc
     1801 gtcgggcggc agttccaggc ccgacgtggg ctgcgggcgc ggctggtcgg cctggtcacc
     1861 ctgtgcccac ggcgagttgt gcggattgct cacgacgtcc cccttctgac catcccagaa
     1921 taggtggccg gtggctcgca ccccggccgg gtcagagtcg gtacccgtcg tcgccgtccc
     1981 tgcgcaccag cccgtcgtcc accagggact gcaacgcgcg gacggcctgc gcacggtcgg
     2041 gccagccctc gagcagccgg ggttcggttg ctgccgcgtc cgtgggaagg gcgcgcaggt
     2101 gcgccagcag atgcccgcgg gcggcccgat cggtgccctc gtacaactgc cgacgcctgg
     2161 gcgggccggg cgggcgtccg gcggcgagcc agcggcagtc gttctgcacg gggcaggcgg
     2221 agcacagcgg gtcaccggcc cggcacacct gagccccgag ttccatcgcg gccaccgacc
     2281 agcgggcggc accggcatcc ggcagccacg cctccgcccg gcgtcgttcg gcctgggtgg
     2341 gtgcggcagc gggagcctct gtgccgcctt ccagccgggc caggacgcgg cgcacattga
     2401 cgtcgagcac cacctgacgt gcgccgaagg cgaaactggc cacggcagcg gcggtgtagt
     2461 caccgacgcc gggcagagtg cgcaacaccg cagggtcgga cggcaccctg ccctcgtgtt
     2521 cggacgtgat cgcggtggcc gcggcatgca ggcgctgagc ccggcgcggg tagcccagcc
     2581 gtccccacgc ccggagggcc tcgccgaccg gttccgcggc cagtgcggcc ggtgtcggcc
     2641 accggctcac ccactgctgc cagggctgca ggacgcgccg gaccggtgtc tgctgcagca
     2701 tgaactcgct gaccagcacc gcccacggcg aggtgtccgg acgccgccac ggcaggtcac
     2761 gcgcgtgctc gccgtaccag gcgacgatgc ttgccaccag ccggctgcgc tccaccgcac
     2821 cactgtagaa cgtgccgctc agcaggcccg gtgtgggttg cgtcaagccg ctcgggggcc
     2881 ttgcggcgtg gtgtaacgcg cgcgggcgct gcactgccta cccttgcggc atgagcgaag
     2941 tgctgacagg caagggtccg caaccgcctt cggtttactg gattcgccgc ggcctggtcg
     3001 ccctggccgc cgtgatcatc atcggcctgc tggtgtgggc catcgtgccg aagaacgcac
     3061 cgtccacggc caccccggcg gcgaacagcc ccaccccggc acccagcgca ctcgacacca
     3121 cgacgtcagc cggggcgtcg ccgaccgcct cgccgagcga tggcaccacg agccccagcc
     3181 ccaccacctc ggccgcggcg gccgctgcct gcgacccgat cggggtgcaa ctgagcgtcg
     3241 agggcttcaa gagcgtcaag gccgacgcca agcaggtctt cagcgtgact gtcgagaaca
     3301 acaccgcact gccgtgcctg atgcagatca ccaaggacac cttccggttg cgggtcgctt
     3361 ccggcaaaga catgatctgg agcaccgcgc actgcgacac gtggctgccc gaggtcaagc
     3421 agcaaacact caaggccggc gcagcggtcg agttcaagat caactggtca ctgcaccgct
     3481 cggcggaggg atgcaagcag accaaggacg tgctgggcgc cggcacctac gtcgccaccg
     3541 ccatgtaccg cgacaccgcc accggcaagt ttcctttcac gatcaccaag taggcgcagt
     3601 atcgcgggtg gagcttgtcg agattctcgt gaggggcgcc gtatcgctgg tcgcgtttgc
     3661 tgagacctcg tgatcaggga tatcaacagg gtcgaccaac ggatcgctgc gtgagtactc
     3721 gctcagcgct ggtcgagcag gcgggatcct cgtggctcgc gcagtatcgc tggtctgtcg
     3781 agacctcgtg agcccccggg caagctcgac cagccacccg tgtcggaatg caccctgcgc
     3841 tcagtccagc cgctccgtgt atgcggactc ggccagccgg atcagtccct cgcgcacggt
     3901 gcgggcgatc gagtagtcca cgccgtccac gcccaacagg tcggccgtgg tggcgccgaa
     3961 cagcccctgc agcgtgccga agtgggccac cagcgagtcg gcgaccggtg gcgcgagccg
     4021 tccgatctgc gccagctgcc ggtgcccctg gggctgcata cgtcgggaca ggtcatcgcc
     4081 caggccgcag gcctgggcga tcaggccggg cgaggcaaga tcgtcggcct caagaccggc
     4141 cagcctggcg agttggggtg tggtggtgtc gtcggtgtag tcgcgctgca ggtgagtgga
     4201 cagctcgtcc aggcccgaga cgagctcttg caactgcaac cgcaccaggc ggccgtcggt
     4261 gcccagttcg acggcgtagc cggtgacctc gagccgaagc cggcgcacca tttcgagccg
     4321 ctgcgcgacc agggcgatgt cgcgcaccgt tgcggcgtcc tgcacctcgg ctgccgacag
     4381 ccgcgcggtg gcctcaccca gccggtcgga gaagcgcacc agggtggcga gcgcctggtt
     4441 ggcccgggcc agcagctgct caggcctctg caccacccag cgacgttcgt cgacgaacag
     4501 cgagatggtc gacatggacg ccgacacggt gaccgtcggt acgcccgtct gacgggccac
     4561 ccggtcggcg gtgcggtgcc gggtgccggt ctcggaggtg gcgacggcgg cgtcgggcat
     4621 caggtgcacg cccgcgaaca ggatgcgatc gagggcgtcg tcgagaatga tcgcgccgtc
     4681 catcttggcg agttcgcgca gggcggtgcc ggtgaaggtc acgtcgatgg cgaagc
//
LOCUS       DAOSUG010000213         4727 bp    DNA     linear   ENV 10-JUN-2024
DEFINITION  MAG TPA_asm: Micropruina sp. isolate AMR_MDS_2195
            NODE_7847_length_4727_cov_3.814854, whole genome shotgun sequence.
ACCESSION   DAOSUG010000213 DAOSUG010000000
VERSION     DAOSUG010000213.1
DBLINK      BioProject: PRJNA925477
            BioSample: SAMN32864262
            Sequence Read Archive: SRR4236673
KEYWORDS    WGS; ENV; Metagenome Assembled Genome; MAG; Third Party Data; TPA;
            TPA:assembly.
SOURCE      Micropruina sp. (wastewater metagenome)
  ORGANISM  Micropruina sp.
            Bacteria; Bacillati; Actinomycetota; Actinomycetes;
            Propionibacteriales; Nocardioidaceae; Micropruina.
REFERENCE   1  (bases 1 to 4727)
  AUTHORS   Abdulkadir,N., Saraiva,J.P., Zhang,J., Stolte,S., Gillor,O.,
            Harms,H. and Rocha,U.
  TITLE     Genome-centric analyses of 165 metagenomes show that mobile genetic
            elements are crucial for the transmission of antimicrobial
            resistance genes to pathogens in activated sludge and wastewater
  JOURNAL   Microbiol Spectr 12 (3), e0291823 (2024)
   PUBMED   38289113
REFERENCE   2  (bases 1 to 4727)
  AUTHORS   Abdulkadir,N.
  TITLE     Direct Submission
  JOURNAL   Submitted (24-MAR-2023) Environmental Microbiology, Helmholtz
            Center for Environmental Research -UFZ, Permoserstr 15, Leipzig
            04318, Germany
COMMENT     The annotation was added by the NCBI Prokaryotic Genome Annotation
            Pipeline (PGAP). Information about PGAP can be found here:
            https://www.ncbi.nlm.nih.gov/genome/annotation_prok/
            
            ##Genome-Assembly-Data-START##
            Assembly Method        :: SPAdes v. 3.11.1
            Genome Representation  :: Full
            Expected Final Version :: Yes
            Genome Coverage        :: Not Applicable
            Sequencing Technology  :: Illumina MiSeq
            ##Genome-Assembly-Data-END##
            
            ##Genome-Annotation-Data-START##
            Annotation Provider               :: NCBI
            Annotation Date                   :: 05/04/2023 04:57:10
            Annotation Pipeline               :: NCBI Prokaryotic Genome
                                                 Annotation Pipeline (PGAP)
            Annotation Method                 :: Best-placed reference protein
                                                 set; GeneMarkS-2+
            Annotation Software revision      :: 6.5
            Features Annotated                :: Gene; CDS; rRNA; tRNA; ncRNA
            Genes (total)                     :: 2,776
            CDSs (total)                      :: 2,750
            Genes (coding)                    :: 2,714
            CDSs (with protein)               :: 2,714
            Genes (RNA)                       :: 26
            tRNAs                             :: 24
            ncRNAs                            :: 2
            Pseudo Genes (total)              :: 36
            CDSs (without protein)            :: 36
            Pseudo Genes (ambiguous residues) :: 0 of 36
            Pseudo Genes (frameshifted)       :: 2 of 36
            Pseudo Genes (incomplete)         :: 32 of 36
            Pseudo Genes (internal stop)      :: 2 of 36
            ##Genome-Annotation-Data-END##
FEATURES             Location/Qualifiers
     source          1..4727
                     /organism="Micropruina sp."
                     /mol_type="genomic DNA"
                     /submitter_seqid="NODE_7847_length_4727_cov_3.814854"
                     /isolate="AMR_MDS_2195"
                     /isolation_source="Activated sludge of municipal
                     wastewater treatment plants"
                     /db_xref="taxon:2737536"
                     /environmental_sample
                     /geo_loc_name="Canada"
                     /lat_lon="49.25 N 123.1 W"
                     /collection_date="2015-06-16"
                     /metagenome_source="wastewater metagenome"
                     /note="metagenomic"
     gene            <1..711
                     /locus_tag="PLF56_07950"
     CDS             <1..711
                     /locus_tag="PLF56_07950"
                     /inference="COORDINATES: protein motif:HMM:NF022007.2"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="helix-hairpin-helix domain-containing protein"
                     /protein_id="HOQ53532.1"
                     /translation="VVAPSETGDDDDLTDDIPAVRASGSQRPGWLPTMGPAVRQFVRE
                     HLVVIGIIVLSGCLWGGYSLFQARTTAVAAPTTPPSVQVSVATPSASATPVLLVHVLG
                     EVRRPGVVTLPGGSRVQDALKAAGGLTDKAVPGDLNLAAPVSDGTQLVIGRKGSQVRA
                     STAASGTSTAAADPTLDLNTATLEQLDTLPGVGPVTAAKILAWREAHQRFRSVAELQE
                     VDGIGPKSYAQIAPRVRV"
     gene            698..3061
                     /locus_tag="PLF56_07955"
     CDS             698..3061
                     /locus_tag="PLF56_07955"
                     /inference="COORDINATES: protein motif:HMM:NF015714.2"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="ComEC/Rec2 family competence protein"
                     /protein_id="HOQ53533.1"
                     /translation="MSGSEQARSDRITAGRADGSSAPDLRLVPAALAAWAGMWVATAA
                     QPAWLTLGTLLGCATGLLGRRRRSWRLAAVALVLVGCMATGWARWWWQQHDPLVALAA
                     QQAVATADLVVVGTPRMGQREGVRPPWWLSAARVVSLDSRGERMAGGAVVQLSASGEQ
                     VAAWAAVVPGTTVRAVVRLGMAGPGEQPEVQVRAREPPVVVAAPGPLDAAVEQVRAGL
                     RASVAHVSHEPRALVPALVVGDTSAMPEGLVERFRVTGLTHLTAVSGANLTLLLAFLG
                     TLARACGVLGWWLRGLLTLGVVGFVVLCHAEPSVVRAAAMGVVGLVALGAGGRGGQAV
                     RALCVAVMVIVFVEPTMARSAGFALSVLASGGIVAWSRPWTDLLAMRMPRLLAEGLAT
                     PLAAQLATQPLVTAISGQVSIVGLVANLAAGPLVGPATVLGFLAAASALPLLPLAMVF
                     GTLAGWCAQGLCWIAGLGGLFPGAAVSWPAGPIALLLVLAACVGLVLVMPRLLRNPWL
                     ASGCALLLVAAMLRPPAPPGWPPHDWAVVFCDVGQGDATLIRSAPAEVVIVDAGPDPA
                     KLATCLRDLGVARVPLAVLTHFHADHVTGIEVALQNHHTDRVLVSAANSPWSGRQLVE
                     RAASGVGLTQVTPGSVVQAGAVRLEVLAVRTAVDVTSSEEGESAGENDGSLVMRFTVP
                     GLRLLLAGDVQEDGQRNAVSSGADLSADVLLVPHHGSGHQLPEFLASTRATIAVFSVG
                     LDNDYGHPAVRTQRAVEQLGMRVLRTDQQGSIALAVTESGLVVTTQR"
     gene            complement(3384..4547)
                     /locus_tag="PLF56_07960"
     CDS             complement(3384..4547)
                     /locus_tag="PLF56_07960"
                     /inference="COORDINATES: protein motif:HMM:NF012382.2"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_function: GO:0030170 - pyridoxal phosphate binding
                     [Evidence IEA];
                     GO_process: GO:0009058 - biosynthetic process [Evidence
                     IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="aminotransferase class I/II-fold pyridoxal
                     phosphate-dependent enzyme"
                     /protein_id="HOQ53534.1"
                     /translation="MSILTAPAITAAEAVRRIEAVSRRVQQPASSGDLVSLAVGEPDF
                     TTPAPIVEAAAAALRDGWTHYAPLKGDPQLRFALADQVGTLLGRPANPDHIQVTHGGS
                     AGLASAILGLIDPGDVVVLPDPTYSLYADLIAMAGGRAVHVPLADDLHWDLDALTSAL
                     TGARMFVFCNPSNPTGIVHTRAELEALGDALDGTDTLVLTDEAYADLVFTSAPFVSAL
                     EVPSLAERTIYCQTFSKSYAMTGWRIGYLWGPPAVIEAAGRVHASFMGSPNTFVQRAG
                     LVALSDCADEVAGMREAYRRRQRLMYDELRVVTGLQVQPPEGAFYLFPRYDAPIGSIE
                     LVGYLRSHGVAVRPGAEFGKHGEHHLRLSCAASDEHIITGVHRLADALTQITG"
BASE COUNT          619 a         1443 c         1843 g          822 t
ORIGIN      
        1 gttgttgcac cgtccgagac gggcgacgat gacgacctca ccgacgacat ccccgcggtc
       61 cgggcgagcg ggtcgcaacg gcccggctgg ttgccgacga tgggccccgc ggtgcgtcag
      121 ttcgtgcgtg agcacctggt ggtgatcggc atcatcgtgc tgagcggctg tctgtggggc
      181 gggtactcat tgttccaggc acgcaccacg gcggtggccg ctcccaccac ccccccgagc
      241 gtgcaggtta gcgtggccac ccccagcgcc tccgcgacgc cggtgctgct ggtccatgtt
      301 ctgggcgagg tgaggcgtcc gggtgtggtc acccttcccg gcggttcacg ggtgcaagac
      361 gcgttgaagg ccgccggcgg cctcaccgac aaggccgtgc cgggtgatct caacctcgcc
      421 gccccggtca gtgacggcac ccaactggtg atcggacgca agggcagcca ggtgcgtgcc
      481 tccacggcgg cctccggtac ctccaccgca gccgctgatc cgactctcga cctgaacacc
      541 gccacactcg agcaactcga cacgctgccc ggagtcgggc ccgtcacagc ggccaagatc
      601 ctggcctggc gcgaggcgca tcaacgattc cgcagcgtcg ccgaactgca agaggtcgac
      661 ggcatcgggc ccaagagcta cgcccagatc gccccacgtg tccgggtctg agcaggcgcg
      721 cagcgaccgg atcacagccg ggcgcgcaga cggctcgagc gcacccgacc tgcgtctggt
      781 cccggccgcg ctggcggcct gggccggtat gtgggtggcc accgctgctc agcccgcatg
      841 gttgacgctg ggcacactgc tcggctgtgc cacggggctc ctgggtcgtc ggcgccgctc
      901 atggcgactt gcggcggtcg ccctggtgct ggtgggctgc atggcgaccg ggtgggcccg
      961 atggtggtgg caacagcacg atccgctggt cgccctcgct gcacagcagg cggtggccac
     1021 cgcagacctg gtcgtcgtag gcacgccgcg aatgggccaa cgcgagggcg tccgtccgcc
     1081 gtggtggctg agcgcggcca gggtggtgtc gctcgactcg cgcggtgaac gaatggccgg
     1141 cggtgcggtg gtgcagctga gcgcgagcgg cgaacaggtg gcggcctggg ccgcggtggt
     1201 gcctggcacc acggtgcggg ccgtggtgcg gttgggtatg gcggggcctg gcgaacagcc
     1261 cgaggtgcag gtacgcgcca gagaaccgcc ggtggtggtt gctgcgcccg gcccgctcga
     1321 cgcagcagtg gagcaggtgc gagccgggct gcgggcaagc gtcgcgcacg tgtcgcacga
     1381 gccgcgcgcc ttggtgcccg cgctggtcgt tggtgacacc tccgccatgc ccgaggggtt
     1441 ggttgagagg ttccgggtca cgggattgac ccacctgacg gcagtgtcgg gcgcaaacct
     1501 gacgctgttg ttggccttcc tgggcacgct ggcccgggcg tgtggtgtgt tgggttggtg
     1561 gctgcgtggg ttgttgacgc ttggggtggt gggcttcgtg gtgttgtgcc atgccgagcc
     1621 gagcgtggtg cgggccgcgg ccatgggcgt ggtgggtctg gttgcgctgg gtgctggtgg
     1681 gcgtggtggc caggcggtgc gggcgttgtg tgtggcggtg atggtgatcg tgttcgtcga
     1741 gccgacgatg gctcgctcgg caggtttcgc gctgtccgtt ctggccagtg gcggcatcgt
     1801 tgcgtggtct cgtccatgga cggatctgtt ggccatgcgc atgccacgac tgctggcgga
     1861 gggcctggcg acaccgttgg ccgcgcagct ggccacgcag ccgctggtga cggccatctc
     1921 ggggcaggtc agcatcgtcg gcttggtcgc gaatcttgcc gccggcccgt tggtgggtcc
     1981 ggccacggtg ttgggctttc tggcggccgc ctcggcactg cccctgctgc cgcttgccat
     2041 ggtgttcggc acgttggcgg gttggtgtgc gcaggggttg tgttggatcg ccgggctggg
     2101 cggactgttt ccgggtgcgg cggtcagctg gccggccggg ccgattgcgc tgctgttggt
     2161 cctggcggcc tgtgtcggcc tggtcctggt gatgccgagg ctgctgcgca acccgtggct
     2221 ggcgagcggc tgtgcgttgc tgctggtggc ggccatgctt cgtccgccgg caccgcccgg
     2281 ctggccgccc cacgattggg ccgtggtgtt ctgcgatgtc gggcagggcg atgcgacgtt
     2341 gatccggtcg gctcctgctg aggtggtgat cgtggatgca ggccccgatc cggccaagtt
     2401 ggccacctgc ctgcgcgacc tgggcgtcgc gcgcgtgccg ttggcggtgc tgacgcactt
     2461 tcatgccgac cacgtcaccg gcattgaggt cgccttgcag aaccaccaca ccgaccgggt
     2521 gctcgtctcg gccgcgaaca gcccttggtc ggggcggcag ctggtcgaac gggcagcgtc
     2581 cggggtgggg ctgacgcagg tgacgccggg cagtgtggtg caggccggtg cggtgcgact
     2641 ggaggtactc gccgtccgca ccgccgtcga cgtcacgtcc tccgaagaag gcgaatccgc
     2701 gggcgagaac gacgggtcgt tggtgatgcg gttcaccgtg ccgggtctgc gtctgctgtt
     2761 ggccggtgac gtgcaggagg atggccagcg caacgccgtc agctcgggcg cagacctcag
     2821 cgctgacgtg ctgctggtgc cgcaccatgg ctcggggcat cagttgcccg agttcctggc
     2881 gtccaccagg gccacgatcg cggtgttcag tgtgggactc gacaacgact acggtcaccc
     2941 cgcggtgcgc acgcagcggg cggtcgagca actcggcatg cgggtactgc gtaccgatca
     3001 gcagggttcg atcgcactgg ccgtcaccga gtcaggcctc gttgtcacca cccagcggtg
     3061 agcgaacgac aaggtgtcga aatggggctc cggcccgggt gtggtactgc gactggtgtg
     3121 gccggagggg ggagcgcgac tctccatggt gctggcccgg gcgatgccgg cgatgcgcgt
     3181 cgcgcggcga actggtcagc aatcaacgca aagggcagtg gtgagaaccc cccacgatgc
     3241 atcggacggt tgtgcatgac tcggcacatg atgagccgcg gacaagcggt ctgtggcccg
     3301 cgccggtgcg agtggcctac tggcttgccc ggccccgcgc ggacgtgcgt gcgactgggt
     3361 gggcatcgtg gggtttcgcg ggatcacccg gtgatttgcg tcagtgcatc ggcaagccgg
     3421 tgcactccgg tgatgatgtg ttcgtcactg gcggcgcaag acaggcgcag gtggtgttca
     3481 ccatgttttc cgaactctgc tcctggccga acggcaacgc catggctgcg cagatagccg
     3541 accagttcga tcgagccgat cggggcgtcg tagcgaggaa agagatagaa ggcgccttcg
     3601 ggcggttgca cctgcaggcc ggtgaccacg cgcagttcgt cgtacatcaa tcgctgccga
     3661 cgccggtacg cctcgcgcat accggccacc tcgtccgcgc agtcgctgag ggcgaccagg
     3721 cctgcccgct gtacgaaggt gttgggcgag cccatgaagc tggcgtggac gcgtccggct
     3781 gcttcgatca ccgccggcgg accccacagg tagccgatgc gccagccggt catcgcgtag
     3841 ctcttggaga aagtctggca gtagatggtg cgctcagcca ggctgggcac ctcgagcgct
     3901 gacacgaatg gtgcgctggt gaagaccagg tcggcgtagg cctcgtcggt gagtaccagg
     3961 gtgtcggtgc cgtccagagc gtcgccgagg gcttcgagtt cggcgcgggt gtgcacgatg
     4021 ccggtggggt tggacgggtt gcagaacacg aacatgcgtg ccccggtcag cgccgaagtg
     4081 agtgcatcga ggtcccagtg cagatcgtcg gcgagtggca cgtgcacggc gcgtccgccg
     4141 gccatggcga tcaggtcggc gtacagcgag taggtggggt cgggtaaaac gacgacgtcg
     4201 ccgggatcga tcaggccgag gatcgctgag gcgagacctg cggagccgcc gtgggtgacc
     4261 tggatgtggt cggggttggc gggccgtccg agcagggtgc cgacctggtc ggcgagtgca
     4321 aaccgtagct gcgggtcgcc cttgagtggc gcgtagtggg tccagccgtc gcggagcgcg
     4381 gcggctgctg cctcgacgat gggtgcgggg gtggtgaagt cgggttcgcc gaccgcgagc
     4441 gagacgaggt cgccggagga ggcgggctgc tgcacgcggc gcgaaaccgc ttcgatgcgc
     4501 cgtacagcct cggcggcggt gatagcgggc gcggtcagga tgctcatggt ggtgcctttc
     4561 gggctgggtt ggccgggtgg atcgttggag tgtggtcagt tgtctgcgag gggggcgccg
     4621 atgctgcggc tgagggcgac gtcagagctg gtgtcccgct gccgggtggg tgcgacgacg
     4681 atctcctggg cgcaggctcg cggcggcagg cagagggcca tcaggat
//
LOCUS       DAOSUG010000214         4709 bp    DNA     linear   ENV 10-JUN-2024
DEFINITION  MAG TPA_asm: Micropruina sp. isolate AMR_MDS_2195
            NODE_7873_length_4709_cov_4.106360, whole genome shotgun sequence.
ACCESSION   DAOSUG010000214 DAOSUG010000000
VERSION     DAOSUG010000214.1
DBLINK      BioProject: PRJNA925477
            BioSample: SAMN32864262
            Sequence Read Archive: SRR4236673
KEYWORDS    WGS; ENV; Metagenome Assembled Genome; MAG; Third Party Data; TPA;
            TPA:assembly.
SOURCE      Micropruina sp. (wastewater metagenome)
  ORGANISM  Micropruina sp.
            Bacteria; Bacillati; Actinomycetota; Actinomycetes;
            Propionibacteriales; Nocardioidaceae; Micropruina.
REFERENCE   1  (bases 1 to 4709)
  AUTHORS   Abdulkadir,N., Saraiva,J.P., Zhang,J., Stolte,S., Gillor,O.,
            Harms,H. and Rocha,U.
  TITLE     Genome-centric analyses of 165 metagenomes show that mobile genetic
            elements are crucial for the transmission of antimicrobial
            resistance genes to pathogens in activated sludge and wastewater
  JOURNAL   Microbiol Spectr 12 (3), e0291823 (2024)
   PUBMED   38289113
REFERENCE   2  (bases 1 to 4709)
  AUTHORS   Abdulkadir,N.
  TITLE     Direct Submission
  JOURNAL   Submitted (24-MAR-2023) Environmental Microbiology, Helmholtz
            Center for Environmental Research -UFZ, Permoserstr 15, Leipzig
            04318, Germany
COMMENT     The annotation was added by the NCBI Prokaryotic Genome Annotation
            Pipeline (PGAP). Information about PGAP can be found here:
            https://www.ncbi.nlm.nih.gov/genome/annotation_prok/
            
            ##Genome-Assembly-Data-START##
            Assembly Method        :: SPAdes v. 3.11.1
            Genome Representation  :: Full
            Expected Final Version :: Yes
            Genome Coverage        :: Not Applicable
            Sequencing Technology  :: Illumina MiSeq
            ##Genome-Assembly-Data-END##
            
            ##Genome-Annotation-Data-START##
            Annotation Provider               :: NCBI
            Annotation Date                   :: 05/04/2023 04:57:10
            Annotation Pipeline               :: NCBI Prokaryotic Genome
                                                 Annotation Pipeline (PGAP)
            Annotation Method                 :: Best-placed reference protein
                                                 set; GeneMarkS-2+
            Annotation Software revision      :: 6.5
            Features Annotated                :: Gene; CDS; rRNA; tRNA; ncRNA
            Genes (total)                     :: 2,776
            CDSs (total)                      :: 2,750
            Genes (coding)                    :: 2,714
            CDSs (with protein)               :: 2,714
            Genes (RNA)                       :: 26
            tRNAs                             :: 24
            ncRNAs                            :: 2
            Pseudo Genes (total)              :: 36
            CDSs (without protein)            :: 36
            Pseudo Genes (ambiguous residues) :: 0 of 36
            Pseudo Genes (frameshifted)       :: 2 of 36
            Pseudo Genes (incomplete)         :: 32 of 36
            Pseudo Genes (internal stop)      :: 2 of 36
            ##Genome-Annotation-Data-END##
FEATURES             Location/Qualifiers
     source          1..4709
                     /organism="Micropruina sp."
                     /mol_type="genomic DNA"
                     /submitter_seqid="NODE_7873_length_4709_cov_4.106360"
                     /isolate="AMR_MDS_2195"
                     /isolation_source="Activated sludge of municipal
                     wastewater treatment plants"
                     /db_xref="taxon:2737536"
                     /environmental_sample
                     /geo_loc_name="Canada"
                     /lat_lon="49.25 N 123.1 W"
                     /collection_date="2015-06-16"
                     /metagenome_source="wastewater metagenome"
                     /note="metagenomic"
     gene            complement(<1..476)
                     /locus_tag="PLF56_07965"
     CDS             complement(<1..476)
                     /locus_tag="PLF56_07965"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_011754424.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="magnesium chelatase"
                     /protein_id="HOQ53535.1"
                     /translation="MTDRPTISTLRELRASGHEQKHLRDEIRDNLLAALAEGYTPWPG
                     LHGFERTVLPQLERALIAGHDVVLLGERGQGKTRLLRTLEGLLDEWTPVIAGSELGEH
                     PYGPITAESKRRAAELGDELPVAWRHRSERYAEKLATPDTSVADLIGDVDPIRVAEG"
     gene            803..1423
                     /locus_tag="PLF56_07970"
     CDS             803..1423
                     /locus_tag="PLF56_07970"
                     /inference="COORDINATES: protein motif:HMM:NF012521.2"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="histidine phosphatase family protein"
                     /protein_id="HOQ53536.1"
                     /translation="MPSPKTLRVLLIRHGQTPTTGQILPGRAPGLHLSEHGRTQAEAV
                     AQRLTGVNLAALYTSPLERARETAAPTEAATGKTATVEPALLECDFGDWTGEQLSSLA
                     KLKQWRLVQTAPSTFRFPGGESFVELQTRMIALLERLRIAHPGGIVACFSHADPIKAL
                     LTHAMGTHLDHFQRVDVGTGSISVIDWPGDGAPIVRTVNSTHGPLP"
     gene            1420..2166
                     /locus_tag="PLF56_07975"
     CDS             1420..2166
                     /locus_tag="PLF56_07975"
                     /inference="COORDINATES: protein motif:HMM:TIGR03843.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="SCO1664 family protein"
                     /protein_id="HOQ53537.1"
                     /translation="MSVPDDVPRSPEGPTEPAVREVMLESGELAMVGRVVDSSNATYV
                     VEVTQGDDYAWAIYKPLAGERPLWDFEPGLYKRERAAYLLSEHLGWGLVPPTVIRADA
                     PLGAGSLQWFIDFDPAAHFFTLMRHHPDSHDALRRMAVFDVVSNNTDRKGGHVLRDAE
                     GGVWGIDHGLCFAAEFKLRTVIWDFAGEQVPDDLLDDLAPLADEMPECIAELLTEREV
                     RALRRRVQRLLGSRMMPLDTTGHAYPWPLM"
     gene            complement(2448..2690)
                     /locus_tag="PLF56_07980"
     CDS             complement(2448..2690)
                     /locus_tag="PLF56_07980"
                     /inference="COORDINATES: protein motif:HMM:NF014636.2"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="MoaD/ThiS family protein"
                     /protein_id="HOQ53538.1"
                     /translation="MRIRYFAGAAEAAGVSEETVEADFEVTVAELVEQLGAERERLAK
                     VLAVSTVLIDGQAARERTEYVGRHAQVDVLPPFAGG"
     gene            complement(2693..3730)
                     /gene="moaA"
                     /locus_tag="PLF56_07985"
     CDS             complement(2693..3730)
                     /gene="moaA"
                     /locus_tag="PLF56_07985"
                     /EC_number="4.1.99.22"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_016456386.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_function: GO:0051539 - 4 iron, 4 sulfur cluster binding
                     [Evidence IEA];
                     GO_process: GO:0006777 - Mo-molybdopterin cofactor
                     biosynthetic process [Evidence IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="GTP 3',8-cyclase MoaA"
                     /protein_id="HOQ53539.1"
                     /translation="MTVDVGVPLRLADTHGRVATDLRVSLTDRCNLRCTYCMPADGLQ
                     WFPTDEVLSDAEILRVLDVAVRQLGITKIRFTGGEPLLRRSLEQIVAGAKQLRTRTGG
                     SPELSLTTNGIGLDKRVQALAAAGLDRVNISLDSLDRERYAALARRDRLPAVLAGLAA
                     SEAAGLLPVKVNAVIMRGHNEADVVPLAQFCLERGYQLRYIEHMPLGPQHEWDRTTMV
                     TQAEILALLEHRYTLTPAAEPRGAAPAELWQVDADDTQPGGLLGVIPSVTAPFCGACD
                     RTRITADGQLRTCLFSRTETDLRTPLRAGASDRELAELWTGAQARKAPSHGIGEPGFV
                     QPTRTMSAIGG"
     gene            3824..4531
                     /locus_tag="PLF56_07990"
     CDS             3824..4531
                     /locus_tag="PLF56_07990"
                     /inference="COORDINATES: protein motif:HMM:NF024248.2"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="helix-turn-helix domain-containing protein"
                     /protein_id="HOQ53540.1"
                     /translation="MSTPAPDTTRLGATRRRVLAHVQGEADPVRIESIATALRLHANT
                     VRFHLDALQQAGLVRRSIERQGQQGRPHALFSAAPGLSFYPDLAHHGLVLALLARVQA
                     DDAPDRPIAEEIGRGWGESMLEPGQRADAEGLLAVVNELGLTSRLSQQPDGLQLHIVR
                     CPFREFTTTGDPTVCHIHLGLIRGYLAAGGSDLTVTDLQPWVTPELCVAHLSTRSPGN
                     VAAHPTPESVPSSATPT"
BASE COUNT          727 a         1641 c         1630 g          711 t
ORIGIN      
        1 ccctcggcca ctcggatcgg gtcgacgtca ccgatcaggt cggccacgga cgtgtcgggc
       61 gtcgccagtt tttcggcgta ccgctcggag cggtgccgcc aggccacggg aagctcgtcg
      121 cccagctcgg cggcccggcg tttgctctcg gcggtgatcg ggccataggg gtgctcgccg
      181 agctcggagc cggcgatcac cggcgtccac tcgtccagca ggccctccag cgtgcgcagc
      241 agccgggtct tgccctggcc gcgctcaccc agcagcacca cgtcgtggcc ggcgatcagg
      301 gcacgttcga gttgcggcag caccgtccgc tcgaagccgt gcaggccggg ccagggggtg
      361 tagccctcgg ccagggcggc cagcaggttg tcgcggatct cgtcgcgcag gtgcttctgc
      421 tcgtgcccgc tggcacgcag ttcgcgcagg gtgctgatgg tggggcggtc ggtcacgacc
      481 tcacgctaac ccggtaggca agcattgccc ttcatctcat cgggcgtagc gccacgggtc
      541 gcacatgggt ggaacgcatg ggtggagcgc atgggtggag cgcatgggtg gagcgctggg
      601 ccggcacagg tcgccatccg agtcgggatg gaactggacg gcgccgatca tgggttcccc
      661 ggaatctgcc gccctcgtcg cgtcgaggac gatcaccacg tggacgtcgc gccacggtcc
      721 gtccggctgc cccatgagcg cggcccggat tgcaccccgt ccggtgaacg tggccttggc
      781 gacgccgggg tacgctcgac ccgtgccgtc ccccaagacg ctgcgggtgc tgctgattcg
      841 ccacgggcag acccccacca ccggccagat cctgcccggc cgcgcgcccg gactgcacct
      901 ctccgagcac ggacgcaccc aggccgaggc ggtcgcgcag cgattgaccg gggtgaacct
      961 ggcggccctc tacacctcgc cgctcgagcg ggcccgtgag accgccgcgc cgacggaggc
     1021 ggcgaccgga aagaccgcaa cagtggagcc ggcgctgctg gagtgcgact tcggtgactg
     1081 gacgggcgaa caactcagtt cgctcgccaa gctgaagcaa tggcgcctcg tgcagacggc
     1141 gccgtccacg ttccggtttc ccggcggcga gagcttcgtc gaactgcaga cccgcatgat
     1201 cgccctgctg gaacgactgc ggatcgccca tcccggcggc atcgtggcct gtttttcgca
     1261 cgccgacccg atcaaagcgc tgctcacgca cgcgatgggc acgcacctcg atcatttcca
     1321 gcgggtcgat gtgggcacgg gatccatctc ggtgatcgac tggcctggcg acggggcgcc
     1381 gatcgtccgc acggtgaact cgacccacgg acccctgccg tgagcgtgcc ggacgacgtg
     1441 ccccgcagcc cggagggtcc gaccgaaccg gccgtgcgcg aggtcatgct cgagagcggt
     1501 gagcttgcca tggtcggacg ggtcgtcgac agcagcaacg ccacctacgt cgtcgaggtg
     1561 acccagggcg acgactacgc gtgggcgatc tacaagccgc tggccggcga acgtccgctg
     1621 tgggacttcg aacccggcct ctacaagcgc gaacgcgccg cctacctgct gagcgagcac
     1681 ctcggctggg ggttggtgcc accgaccgtg attcgcgcgg acgcaccgct gggcgccggt
     1741 tcgctgcagt ggttcatcga cttcgacccg gccgcccact tcttcacact gatgcgacac
     1801 caccccgaca gccacgacgc actgcggcgc atggcggttt tcgacgtggt gagcaacaac
     1861 accgaccgta agggcgggca cgtgctgcgc gacgccgagg gcggggtgtg gggcatcgat
     1921 cacgggttgt gcttcgcggc cgagttcaag ttgcgcacgg tgatctggga tttcgccggc
     1981 gaacaggtgc cggacgacct gctggacgac ctggccccgc tcgccgacga aatgcccgag
     2041 tgcatcgccg agttgctcac cgagcgcgag gtacgagcgc tgcgacgacg cgtccagcgg
     2101 ctgctgggct cgcgcatgat gccactggac accaccgggc acgcctaccc ctggcccctg
     2161 atgtaacact gcccacggca ccgagcccga cgccgaccct gaccccgaca ccggcaccga
     2221 caccgacacc ggcaccgaca ccggcaccga caccgacgtt gcgcgctctc gggctcattg
     2281 cgcgggttcg gacgcgcgca atgagcccgg aaccgcgcaa cgtcggtgga cggacgtcgg
     2341 tggacggacg tcggttgacg gacatcggcg gacggacatg cgccgcccgt catcccgccg
     2401 ggcacgtcct ggctcggaac agcattgtca gcacacctcg ccggcgccta tccgccggcg
     2461 aaggggggca gcacgtccac ctgggcgtgc cggccgacgt actcggtgcg ttcgcgcgcc
     2521 gcctgcccgt cgatcaacac agtggagacg gccagcacct tcgccagccg ctcgcgctcg
     2581 gcgcccaact gctcgacgag ttcggccacg gtcacctcga aatcggcctc gacggtctct
     2641 tcggagaccc cggcggcctc ggccgcaccg gcgaagtagc ggattctcat gatcaacctc
     2701 cgatggcgct catggtgcgg gtgggctgca cgaagcccgg ttcgccgatg ccgtgggacg
     2761 gtgccttgcg ggcctgcgcg ccggtccaca gctcggccag ttcacgatcg gacgcgcccg
     2821 cacgcagcgg ggtgcgcaga tcggtctcgg tgcgtgagaa caggcaggtg cgaagttggc
     2881 cgtccgcagt gatgcgggtg cggtcgcagg cgccgcagaa cggcgccgtg accgaaggga
     2941 tcacgccgag caatccgccg ggctgggtgt cgtcggcgtc gacctgccag agttcggcgg
     3001 gtgccgcgcc ccgcggttcg gcggcgggcg tcaacgtgta ccgatgctcg agcagcgcaa
     3061 gaatttcggc ctgggtgacc atcgtcgtgc ggtcccactc gtgctggggg cccagcggca
     3121 tgtgttcgat gtagcgcaac tggtacccgc gctcgaggca gaactgcgcg agcggcacca
     3181 cgtccgcctc gttgtggccg cgcatgatca ccgcattgac cttgaccggc agcagtccgg
     3241 ccgcctcgga cgccgccagg ccggccagca ccgccggcag ccggtcacgg cgagccaacg
     3301 cggcgtagcg ttcgcgatcg agcgaatcga gcgagatgtt gacccgatcg agaccggccg
     3361 cggctagggc ctgcacccgc ttgtcgagcc cgatgccgtt ggtggtcagc gacagttcgg
     3421 ggctgccgcc ggtgcgggtg cgcagctgtt tggccccggc gacgatctgt tcgagcgaac
     3481 gccgcagcag cggttcgccg ccggtgaagc ggatcttggt gatgcccaac tgccggacgg
     3541 cgacgtcgag aacccgcagt atctcggcgt cgctcagcac ctcatcagtg ggaaaccact
     3601 gcagcccgtc tgcaggcatg caataggtgc aacgcaggtt gcagcggtcg gtgagcgaca
     3661 cgcgcaggtc ggtggcgacc cgtccgtgcg tatcggccag ccgcagcggc acgcccacat
     3721 cgaccgtcat gtccgacagg ttacgcgacg ccgccgacag ccgagagccg gtggttggcc
     3781 ttaagcgctg cgtgggcaca ggccaactgc catcattgag cgggtgagca cgcctgctcc
     3841 tgacaccact cgcctgggcg cgaccaggcg acgggtgctt gctcacgtgc agggcgaggc
     3901 cgatccggtg cggatcgaga gcatcgcgac cgccctgcgc ctgcatgcga acacggtgcg
     3961 ttttcatctg gacgcccttc agcaggccgg cctggtgcga cggtccattg agcgtcaagg
     4021 tcagcaggga cggccccatg cgctgttcag cgcagcgcca ggcctgtcgt tctatccgga
     4081 cctggcccat cacggcctgg tgctcgcgtt gctggcccgg gtgcaggccg acgacgcccc
     4141 cgatcgtccg attgccgaag agatcggacg ggggtggggc gagtcgatgc tggaaccggg
     4201 ccaacgtgcg gacgccgagg gcctgctcgc cgtcgtcaac gagctgggcc tgacctcacg
     4261 gctgtcgcag caaccggacg ggttgcaact gcacatcgtc cgctgcccgt tccgcgagtt
     4321 caccaccacc ggcgacccga cggtctgtca cattcacctc ggcctgatcc gcggctatct
     4381 tgccgccggc ggttcggacc tcacggtgac agacctgcag ccctgggtga cgcctgagct
     4441 gtgcgtggcg catctcagca cccgctcacc ggggaacgtc gcggcgcatc ccacaccgga
     4501 atcggtaccc tcttcggcga ctccgacgta gcggcacctg cgaatcggct acggacagga
     4561 gcctgctgaa caaggctgtt cggagcgagg acgtgcccgg cgggatgagc tgagccgggt
     4621 gcgccgcagc cgacatgact gttcagcagg cttatagggc gtgtcgcata accctgaccg
     4681 cgctgcggcg cacccagtac gcccgctcg
//
LOCUS       DAOSUG010000215         4707 bp    DNA     linear   ENV 10-JUN-2024
DEFINITION  MAG TPA_asm: Micropruina sp. isolate AMR_MDS_2195
            NODE_7877_length_4707_cov_3.928203, whole genome shotgun sequence.
ACCESSION   DAOSUG010000215 DAOSUG010000000
VERSION     DAOSUG010000215.1
DBLINK      BioProject: PRJNA925477
            BioSample: SAMN32864262
            Sequence Read Archive: SRR4236673
KEYWORDS    WGS; ENV; Metagenome Assembled Genome; MAG; Third Party Data; TPA;
            TPA:assembly.
SOURCE      Micropruina sp. (wastewater metagenome)
  ORGANISM  Micropruina sp.
            Bacteria; Bacillati; Actinomycetota; Actinomycetes;
            Propionibacteriales; Nocardioidaceae; Micropruina.
REFERENCE   1  (bases 1 to 4707)
  AUTHORS   Abdulkadir,N., Saraiva,J.P., Zhang,J., Stolte,S., Gillor,O.,
            Harms,H. and Rocha,U.
  TITLE     Genome-centric analyses of 165 metagenomes show that mobile genetic
            elements are crucial for the transmission of antimicrobial
            resistance genes to pathogens in activated sludge and wastewater
  JOURNAL   Microbiol Spectr 12 (3), e0291823 (2024)
   PUBMED   38289113
REFERENCE   2  (bases 1 to 4707)
  AUTHORS   Abdulkadir,N.
  TITLE     Direct Submission
  JOURNAL   Submitted (24-MAR-2023) Environmental Microbiology, Helmholtz
            Center for Environmental Research -UFZ, Permoserstr 15, Leipzig
            04318, Germany
COMMENT     The annotation was added by the NCBI Prokaryotic Genome Annotation
            Pipeline (PGAP). Information about PGAP can be found here:
            https://www.ncbi.nlm.nih.gov/genome/annotation_prok/
            
            ##Genome-Assembly-Data-START##
            Assembly Method        :: SPAdes v. 3.11.1
            Genome Representation  :: Full
            Expected Final Version :: Yes
            Genome Coverage        :: Not Applicable
            Sequencing Technology  :: Illumina MiSeq
            ##Genome-Assembly-Data-END##
            
            ##Genome-Annotation-Data-START##
            Annotation Provider               :: NCBI
            Annotation Date                   :: 05/04/2023 04:57:10
            Annotation Pipeline               :: NCBI Prokaryotic Genome
                                                 Annotation Pipeline (PGAP)
            Annotation Method                 :: Best-placed reference protein
                                                 set; GeneMarkS-2+
            Annotation Software revision      :: 6.5
            Features Annotated                :: Gene; CDS; rRNA; tRNA; ncRNA
            Genes (total)                     :: 2,776
            CDSs (total)                      :: 2,750
            Genes (coding)                    :: 2,714
            CDSs (with protein)               :: 2,714
            Genes (RNA)                       :: 26
            tRNAs                             :: 24
            ncRNAs                            :: 2
            Pseudo Genes (total)              :: 36
            CDSs (without protein)            :: 36
            Pseudo Genes (ambiguous residues) :: 0 of 36
            Pseudo Genes (frameshifted)       :: 2 of 36
            Pseudo Genes (incomplete)         :: 32 of 36
            Pseudo Genes (internal stop)      :: 2 of 36
            ##Genome-Annotation-Data-END##
FEATURES             Location/Qualifiers
     source          1..4707
                     /organism="Micropruina sp."
                     /mol_type="genomic DNA"
                     /submitter_seqid="NODE_7877_length_4707_cov_3.928203"
                     /isolate="AMR_MDS_2195"
                     /isolation_source="Activated sludge of municipal
                     wastewater treatment plants"
                     /db_xref="taxon:2737536"
                     /environmental_sample
                     /geo_loc_name="Canada"
                     /lat_lon="49.25 N 123.1 W"
                     /collection_date="2015-06-16"
                     /metagenome_source="wastewater metagenome"
                     /note="metagenomic"
     gene            complement(339..947)
                     /locus_tag="PLF56_07995"
     CDS             complement(339..947)
                     /locus_tag="PLF56_07995"
                     /inference="COORDINATES: protein motif:HMM:TIGR03083.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="TIGR03086 family metal-binding protein"
                     /protein_id="HOQ53541.1"
                     /translation="MTNTQNLPDTGPLFLTAFDQLTEVVYRVAPGDLGLPTPCVGWTV
                     RDLLSHLVAVEDRVAHIAQGGQAFEVPSQVDGIADDAWVAAWDDRSPRVRAAVTDDAD
                     PGRIVDHPAGRLPWAVAVGIYSSELATHTWDLARALGVDAELDQQVAAAVLGPIQDAL
                     PAQPRGEEVGIPFGPVVPVADDASSYEQLVGWLGRDPEWAAA"
     gene            1010..2005
                     /locus_tag="PLF56_08000"
     CDS             1010..2005
                     /locus_tag="PLF56_08000"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_012919856.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="YafY family protein"
                     /protein_id="HOQ53542.1"
                     /translation="MRADRLLQIMALLRRHGRMPARRLAEILEVSERTILRDMEALSS
                     AGVPVYAERGRGGGCALLPGYRTEVSGLSPAEAQALFAWTSSSGASELGLSGELASAL
                     TKLSATVPAPALEQAEALASVVMVDRRRWFDDADEVPVLPLLRDAATRRRRVVLTYAA
                     ARSGLSDDTRPPSRRTVDPYGLVENAGRWYLLAGRDGEQRTYRVSRIVAAEVLDEPAQ
                     VPPGFNLAEAWAEQRAALEHLSESCAITLALDPAIVEVFVTHARFQLAAGTQIVHTGI
                     DGDWPVVELTFRAWRAAIAIVLAYGGQARLLSPDWLRARALTLARAAVEGLSSAP"
     assembly_gap    2119..2218
                     /estimated_length=100
                     /gap_type="within scaffold"
                     /linkage_evidence="paired-ends"
     gene            complement(2495..3634)
                     /locus_tag="PLF56_08005"
     CDS             complement(2495..3634)
                     /locus_tag="PLF56_08005"
                     /EC_number="2.3.1.-"
                     /inference="COORDINATES: protein motif:HMM:NF012514.2"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_function: GO:0008080 - N-acetyltransferase activity
                     [Evidence IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="GNAT family N-acetyltransferase"
                     /protein_id="HOQ53543.1"
                     /translation="MGGVEPVIRPATEVDRASINAVEQAADRSFTGLITLPDAAPRDV
                     EFDLPGFVLVAESAGRVVGFAQVLDFDGRFHLEQLSVHPDRQRQGLGARLVAAAEREV
                     VVRGGSVVTLRTFADVPFNAPFYRRHGYVESELPVALQGLVEAERRLGLLAAGTRLTM
                     AKQVAPDVVAVPAVSVLPLRDGPSGLEVFVQFRATTMDFAAGAVVFPGGRLDPADRAV
                     CVPTTHLTAWAATGLPAPGLLAAAAIREVAEECGVLLTPDDLVPWDDWVTPPGGRRRF
                     DVAFYLTAARAGQRWRNTTTEALRAQWRGPQQLLDAAAAGRLRLMPPTRALLAELATL
                     TDVATALALRPTIVPVLHDDESRGRRALRLIREGNGSSPRSSADR"
     gene            complement(3640..3933)
                     /locus_tag="PLF56_08010"
     CDS             complement(3640..3933)
                     /locus_tag="PLF56_08010"
                     /EC_number="5.4.99.5"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_013862120.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_process: GO:0046417 - chorismate metabolic process
                     [Evidence IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="chorismate mutase"
                     /protein_id="HOQ53544.1"
                     /translation="MTAPDPELARLRGSIDNLDSALVCLLAERFKITQRVGELKAELG
                     LPPADPGREAQQIERLRRLAHDAHLDPEFAEKFLTFIVSEVVRHHEQIARNAG"
     gene            4051..4527
                     /locus_tag="PLF56_08015"
     CDS             4051..4527
                     /locus_tag="PLF56_08015"
                     /inference="COORDINATES: protein motif:HMM:NF017355.2"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="toxin-antitoxin system HicB family antitoxin"
                     /protein_id="HOQ53545.1"
                     /translation="MELQPYLNRIAADLDRVTALADQPTRETSRRLAAALEPGLRLAM
                     TELLADAAAELSARLGGTVIAVHMDGQQPDLQITEAQPAEPVAPPAPTQVPDETDDGT
                     ARITVRLPDGLKRRAEALAGQAQQSLNTWIVQAVRRATEPTKPTNTHIGRRLTGWA"
BASE COUNT          713 a         1646 c         1573 g          675 t
ORIGIN      
        1 tctcgccggg gatgacaacg cagcgagcgg gcgtactggg tgcgccgcag cgcggtcagg
       61 gttatgcgac acgccctagt catcggccgg tgtcgttggt cggaatcaag cccccgccga
      121 ccgcgcttgt cgcgatcgac ttccgctgat cgagcgtgcc gagatacaca cacccccacc
      181 gaccagtgac gcgccgatca acccgcggtc cgttggttga gcgggtcgaa acccctagcc
      241 gtagcggtgc gaccagctcg accagcgaac gtccgccccc gaaaaacgga agtcgagatc
      301 atcgtgaggg tctcaagctc gaccagcgaa cgtcccgctc aggctgccgc ccactcaggg
      361 tcgcgcccga gccagccgac cagctgttcg tagctggacg cgtcgtcggc caccggcacc
      421 accgggccga acggaatgcc gacctcttcg ccgcgcggtt gcgccggcag tgcatcctga
      481 atcggcccga gtaccgctgc ggccacctgc tggtcgagtt cggcatcgac cccgagggcg
      541 cgggccaggt cccaggtgtg ggtcgcgagc tcgctgctgt agatgccgac cgccacggcc
      601 cagggaagcc gtccggccgg gtggtcgacg atgcggcccg gatcggcatc gtcggtcacc
      661 gccgcgcgca cccgcggtga ccggtcgtcc caggctgcta cccaggcgtc gtcggcgatg
      721 ccgtccacct gcgagggcac ctcgaacgcc tggccgccct gggcgatgtg cgccacgcgg
      781 tcttcgacgg cgaccagatg gctgagcaga tcgcgcacgg tccagccgac gcagggcgtg
      841 ggcagcccca ggtcgccagg agcgacgcgg tacacgacct cggtgagctg atcgaaggcc
      901 gtgagaaaca gtgggccggt gtccggcagg ttctgagtgt tggtcatgac agcagcttgt
      961 cggcataagc tgacatcttc tgtcatgttt ttctggaaag atcacgtcca tgcgcgccga
     1021 tcgtttgctg cagatcatgg cgctgctgcg acgccacgga cgcatgccgg ctcgtcggct
     1081 ggctgagatt ctcgaggtga gcgagcgcac gatcctgcgc gacatggagg ccctgtcgag
     1141 cgcgggcgta ccggtctacg ccgagcgtgg acggggcggt ggctgtgccc tgctgcccgg
     1201 gtatcgcacc gaggtgagcg gcctgtcacc cgccgaggcg caggcgctgt tcgcctggac
     1261 gtcgagctcg ggcgcgtccg agttggggct cagcggcgaa ctcgcttcgg ccctgaccaa
     1321 gctgtcggcc accgtgcccg caccggcgtt ggagcaggcg gaggccctgg cctcggtggt
     1381 gatggtcgac cgcagacgct ggttcgacga cgcggacgag gtacccgtgc tgcccctgct
     1441 gcgcgatgca gccacccgcc ggcgacgggt cgtactgacc tacgcggccg cccggtccgg
     1501 gttgtccgac gacacccgac cacccagccg gcgaacggtc gacccctacg ggctggtcga
     1561 gaacgccggc cgctggtacc tgttggccgg gcgcgacggc gagcaacgca cctaccgcgt
     1621 ctcacgcatc gtcgccgccg aggtgctgga cgagcccgcg caggtgccgc ccggcttcaa
     1681 cctggccgag gcctgggccg agcagcgcgc ggcgctggaa cacctctccg agtcttgcgc
     1741 gatcacactg gcactcgacc cggcgatcgt ggaggtgttc gtcacccacg cacggttcca
     1801 gttggctgcc ggcacgcaga tcgtccacac gggcattgac ggcgactggc cggtggtgga
     1861 gttgacgttc cgggcctggc gggcggcgat tgcaatcgtg ttggcgtatg gagggcaggc
     1921 acggctgctg tcgcccgatt ggctgcgcgc gcgtgccttg acgctggccc gggcggcggt
     1981 cgaggggctc tcctcagccc cgtgagcccc caccggccgg tcgacccccc gtccacctgg
     2041 gcccggtgaa cgtgagcacg tgcttgagga ccgttccttc tgcttgcaca cccgctgcca
     2101 ccgcgatccc tcgctgggnn nnnnnnnnnn nnnnnnnnnn nnnnnnnnnn nnnnnnnnnn
     2161 nnnnnnnnnn nnnnnnnnnn nnnnnnnnnn nnnnnnnnnn nnnnnnnnnn nnnnnnnntt
     2221 gaggaccgtt ccttctgctt gcacacccgc tgccaccgcg atccctcgct gggatggcga
     2281 tctgaagacc ctccaccagt acgcgggggg cacgctcacc cagcgtcgag gcacgtcact
     2341 cgtgatccct cgctggggtg acgccctgaa aaccctgcac cagtgcgcgg ggtgcgcgct
     2401 cagccagcgt cgaggctcgt cactggtgca ggcttttcag ggcatggctc ctggtcccta
     2461 ctgctgagag gacttgggag cgcacccatc ccggttaccg gtccgcagat gagcggggtg
     2521 aggaaccgtt cccctctcgg atcagccgta gtgcgcggcg tccgcgggat tcgtcgtcat
     2581 gcagcaccgg cacgatcgtg gggcgcagcg ccagcgccgt ggcgacgtcg gtcagggtgg
     2641 cgagctcggc cagcagggcg cgggtgggtg gcatcagccg caggcgtccg gccgcggcgg
     2701 cgtcgagcaa ttgctgcgga ccacgccact gcgcgcgcag cgcctcggtg gtggtgttgc
     2761 gccaccgctg gcccgcgcgc gccgccgtca ggtagaacgc gacgtcgaaa cggcggcgtc
     2821 cgccgggtgg ggtgacccaa tcgtcccagg gcaccaggtc gtcgggggtc agcagcacgc
     2881 cgcattcctc ggccacctcg cggatcgccg ccgccgccag caggccgggc gccggtaacc
     2941 cggtggcggc ccaggcggtc aggtgcgtgg tgggtacaca cacggcccga tcggctgggt
     3001 cgagccgtcc gcccggaaac accacagctc ccgcggcgaa gtccatggtg gttgcgcgga
     3061 actgcacgaa cacctccagc ccgctgggac cgtcgcgcaa cggcagcacg ctcaccgccg
     3121 gcaccgccac cacgtcgggc gccacttgct tggccatggt cagccgggtg ccggcagcca
     3181 gcagccccaa gcggcgctcg gcctcgacca gaccctgcaa ggcaaccggc agttcggact
     3241 cgacgtaccc gtggcgacgg tagaagggtg cgttgaaggg cacgtctgcg aaggtgcgca
     3301 gcgtcaccac cgaaccgcca cgcaccacga cctctcgctc ggccgcggcg accagccggg
     3361 cgccgagccc ttggcgctgc cggtcagggt gcacgctgag ctgctcgagg tgaaaacgtc
     3421 cgtcgaagtc cagtacctgc gcgaaaccga ccacccggcc cgcggactca gcgaccagca
     3481 cgaaaccggg caggtcgaac tcgacgtcgc gcggggccgc gtccggcagg gtgatcaggc
     3541 cggtgaacga cctgtccgcc gcctgctcga ccgcgttgat ggacgcccgg tccacctcgg
     3601 tcgccgggcg gatcaccggt tcaactccgc ccatgtcgct cagcctgcgt tccgggcgat
     3661 ctgctcgtgg tgccgcacga cctcagagac gatgaaggtg aggaacttct cggcgaactc
     3721 ggggtcgagg tgagcatcat gcgcgagtcg tcgcagccgc tcgatctgct gtgcctctcg
     3781 gcccggatcg gcaggcggca ggccgagttc ggccttcaac tcccccaccc gctgggtgat
     3841 cttgaaccgt tcggcgagta ggcacaccag cgccgaatcg aggttgtcga tgctgccacg
     3901 cagccgggcc agttcggggt cgggtgcggt catggcggca tcctaaccgg gtcgcgtccc
     3961 gtcgcagccc acgttcaatg agggccgaga cgccatcatg acgtccgact gacattgaca
     4021 tgacgtctat atggtgtcag tcttgatgtc atggagctac agccctacct gaaccgcatc
     4081 gccgccgacc tcgatcgggt caccgcactg gccgatcagc cgacccgcga gaccagccgc
     4141 cggctggcgg ctgcgctcga gccgggcctc cggctcgcca tgaccgaact gctggccgac
     4201 gccgctgccg agctgtccgc ccggctcggc ggcacggtga tcgccgtgca catggacggc
     4261 cagcagcccg acctgcaaat caccgaggcg caaccggccg agccggttgc accgcccgcc
     4321 cccacgcagg tgccggacga gaccgacgac ggcaccgccc gcatcacagt gcggctaccg
     4381 gacggtctca agcgacgcgc cgaagcgctg gccggccagg cccaacagtc gctcaacacc
     4441 tggatcgtgc aggccgtccg ccgggccact gagcccacga aaccgaccaa cacccacatc
     4501 ggccgccggc tcaccggctg ggcctgagag gaagaccatc atgaatacca ccgcatcgac
     4561 cacccacgag tggcgcttcg ccacggacga acgcccccac ctgcacctgc gcagccaccg
     4621 cggcgacgta cgactgagcc acgacgccgg tccgggcgaa gtgctggtgc ggctgacctc
     4681 gaacgccgcc ttcgacccgc agcaggt
//
LOCUS       DAOSUG010000216         4697 bp    DNA     linear   ENV 10-JUN-2024
DEFINITION  MAG TPA_asm: Micropruina sp. isolate AMR_MDS_2195
            NODE_7889_length_4697_cov_4.774020, whole genome shotgun sequence.
ACCESSION   DAOSUG010000216 DAOSUG010000000
VERSION     DAOSUG010000216.1
DBLINK      BioProject: PRJNA925477
            BioSample: SAMN32864262
            Sequence Read Archive: SRR4236673
KEYWORDS    WGS; ENV; Metagenome Assembled Genome; MAG; Third Party Data; TPA;
            TPA:assembly.
SOURCE      Micropruina sp. (wastewater metagenome)
  ORGANISM  Micropruina sp.
            Bacteria; Bacillati; Actinomycetota; Actinomycetes;
            Propionibacteriales; Nocardioidaceae; Micropruina.
REFERENCE   1  (bases 1 to 4697)
  AUTHORS   Abdulkadir,N., Saraiva,J.P., Zhang,J., Stolte,S., Gillor,O.,
            Harms,H. and Rocha,U.
  TITLE     Genome-centric analyses of 165 metagenomes show that mobile genetic
            elements are crucial for the transmission of antimicrobial
            resistance genes to pathogens in activated sludge and wastewater
  JOURNAL   Microbiol Spectr 12 (3), e0291823 (2024)
   PUBMED   38289113
REFERENCE   2  (bases 1 to 4697)
  AUTHORS   Abdulkadir,N.
  TITLE     Direct Submission
  JOURNAL   Submitted (24-MAR-2023) Environmental Microbiology, Helmholtz
            Center for Environmental Research -UFZ, Permoserstr 15, Leipzig
            04318, Germany
COMMENT     The annotation was added by the NCBI Prokaryotic Genome Annotation
            Pipeline (PGAP). Information about PGAP can be found here:
            https://www.ncbi.nlm.nih.gov/genome/annotation_prok/
            
            ##Genome-Assembly-Data-START##
            Assembly Method        :: SPAdes v. 3.11.1
            Genome Representation  :: Full
            Expected Final Version :: Yes
            Genome Coverage        :: Not Applicable
            Sequencing Technology  :: Illumina MiSeq
            ##Genome-Assembly-Data-END##
            
            ##Genome-Annotation-Data-START##
            Annotation Provider               :: NCBI
            Annotation Date                   :: 05/04/2023 04:57:10
            Annotation Pipeline               :: NCBI Prokaryotic Genome
                                                 Annotation Pipeline (PGAP)
            Annotation Method                 :: Best-placed reference protein
                                                 set; GeneMarkS-2+
            Annotation Software revision      :: 6.5
            Features Annotated                :: Gene; CDS; rRNA; tRNA; ncRNA
            Genes (total)                     :: 2,776
            CDSs (total)                      :: 2,750
            Genes (coding)                    :: 2,714
            CDSs (with protein)               :: 2,714
            Genes (RNA)                       :: 26
            tRNAs                             :: 24
            ncRNAs                            :: 2
            Pseudo Genes (total)              :: 36
            CDSs (without protein)            :: 36
            Pseudo Genes (ambiguous residues) :: 0 of 36
            Pseudo Genes (frameshifted)       :: 2 of 36
            Pseudo Genes (incomplete)         :: 32 of 36
            Pseudo Genes (internal stop)      :: 2 of 36
            ##Genome-Annotation-Data-END##
FEATURES             Location/Qualifiers
     source          1..4697
                     /organism="Micropruina sp."
                     /mol_type="genomic DNA"
                     /submitter_seqid="NODE_7889_length_4697_cov_4.774020"
                     /isolate="AMR_MDS_2195"
                     /isolation_source="Activated sludge of municipal
                     wastewater treatment plants"
                     /db_xref="taxon:2737536"
                     /environmental_sample
                     /geo_loc_name="Canada"
                     /lat_lon="49.25 N 123.1 W"
                     /collection_date="2015-06-16"
                     /metagenome_source="wastewater metagenome"
                     /note="metagenomic"
     gene            <1..322
                     /locus_tag="PLF56_08020"
     CDS             <1..322
                     /locus_tag="PLF56_08020"
                     /inference="COORDINATES: protein motif:HMM:NF024771.2"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=2
                     /transl_table=11
                     /product="enolase C-terminal domain-like protein"
                     /protein_id="HOQ53546.1"
                     /translation="KIFALAEVNGVQAAPHGPSDVSPISLAASIHLGVATHNFAIQEY
                     MGYPDLVHEAFPHAWSVQDGHLQPGDEPGLGVSLNDELHSPDDYEPAYLPVARRRDGT
                     LTDW"
     gene            329..1801
                     /locus_tag="PLF56_08025"
     CDS             329..1801
                     /locus_tag="PLF56_08025"
                     /EC_number="1.1.1.-"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_016670317.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_function: GO:0016616 - oxidoreductase activity, acting
                     on the CH-OH group of donors, NAD or NADP as acceptor
                     [Evidence IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="mannitol dehydrogenase family protein"
                     /protein_id="HOQ53547.1"
                     /translation="MTQALSRSSYTGSVLGSSAPPNPGMLHLGIGNFHRAHAAVYTAK
                     AMAAQGGDWGIVAAANRSRRVVDSLVAQDYLYSILELSSAGERVDVMDVHREALVAAE
                     QTAELMARIASPEIKIVTLTISENGYCRNAVTGDLDIDGALVSADLANPAVPRTSIGQ
                     LAGALIRRYQGDAGPITILSCDNMVSAGHNAHKMVSQYLQATATPEGFHEWFAASVTF
                     PNAMVDRIVPGTTDATRARVRELLGVVDAIPVPAERFTMWVIEDNFAAGRPAWEEAGA
                     IFSDEVEAYELVKLRLLNGSHSLIAYLGGLSGSETIPASFGHDFVRECVTELLNVEYL
                     PSIELPTGFDPQAYIAQLFDRWSNHALGDATARVGSDGSLKLLQRVPDPALRMLDLGQ
                     VPQQLALMVAGWIACVAPPTGFDPGPIAAAMIEPAKDKLAAVTSGAGSVRAHVEAILR
                     GGFFPDALAAHDEFVGRVAELLQIITTHGVRDAAKAALDA"
     gene            1837..2859
                     /locus_tag="PLF56_08030"
     CDS             1837..2859
                     /locus_tag="PLF56_08030"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_016668743.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="L-idonate 5-dehydrogenase"
                     /protein_id="HOQ53548.1"
                     /translation="MKTLKIHGAESIAIEDAPVPEPGEGEVRVRMQYAGICGSDLHYY
                     FHGRNGAFVVNEPFTPGHEMAGVIDLDPSGRWAPGTPVTIAPATYGQPQPGIEDRKYL
                     WPGGSYFGSASTTPHTQGGMSEYRTVKDFMIRELPDGLSLRDASLAEPLAVALHAIRI
                     AGGVEGQKVLVTGSGPIGLCVVAASSALGAAEVTATDMLDGPLQRAAAVGATATLKAG
                     TDEVPAFAFDVVFECSGVAPAVNQALAAVRRAGTVVQVGMLPADKIAVNLEPLVSREV
                     RYLGTFRFDDEIDQAIELLAEHPEIAKVITHVIDADHIVEAFETARNSQESGKVVVSL
                     WLDKEA"
     gene            3246..>4697
                     /locus_tag="PLF56_08035"
     CDS             3246..>4697
                     /locus_tag="PLF56_08035"
                     /inference="COORDINATES: protein motif:HMM:NF012312.2"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_function: GO:0022857 - transmembrane transporter
                     activity [Evidence IEA];
                     GO_process: GO:0055085 - transmembrane transport [Evidence
                     IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="MFS transporter"
                     /protein_id="HOQ53549.1"
                     /translation="MTAANAAGTPSGNVAQRSTKDLAKAAVSGWLGTALEFMDFQLYS
                     LAAALVFKDIFFPSDAPGMAIILAMATYATGYLSRPLGAWYFGRLGDRVGRTKVLFYT
                     IALMGIATTAIGLLPTYHQVGILAPILLVFLRLVQGFGAGAEIAGSSVMLTEYAPRRK
                     RGLVGSLVALGTNSGTLGASAIWGVLVATMSTQTVIEWGWRIPFIASSLIMLFAVWLR
                     RNLKESPVFEGRADVVDGEALSREELDKAAAHDNVLAEALHERKGRAFFEGFFLRFGQ
                     AGNSGMVQTYLISYITAVLLLAPTIGTGVVITSSLVGFATVPLIGWASDFVGRKVMYI
                     TMSSIALVLAVPMIWMIATKNVSLVFAGYIILHQTSVLALASLENNTMSELFGARARL
                     TQLALAKELAAIVATGIGPVAAAALVVATGSWWPIAIMIGFFSICSLGAAIVMPETAG
                     RDLTVLNDARKGEAIIGPFARRERAKLGLPAPEE"
BASE COUNT          750 a         1707 c         1490 g          750 t
ORIGIN      
        1 caagatcttc gccctggccg aggtgaacgg cgtccaggcg gcaccccacg ggccgtccga
       61 cgtctcgccg atctcgctgg cggcgtccat tcatctgggg gtggcgaccc acaacttcgc
      121 catccaggaa tacatgggct accccgattt ggttcacgag gcgttcccgc acgcctggag
      181 cgtccaggac ggccacctgc agcccggcga cgaacccggg ctcggcgtga gcctgaacga
      241 cgaactgcac agcccggacg actacgaacc cgcatatttg ccggtcgcgc gacggcgtga
      301 cggaaccctc acagattggt gatcgacgat gacccaggcc ctgtcccgct ccagctacac
      361 cggctccgtg ctcgggtcga gcgcgccacc caatccgggc atgctgcacc tcggcatcgg
      421 caacttccac cgcgcccacg ccgcggtcta caccgccaag gcgatggccg cgcagggcgg
      481 tgactggggc atcgtcgcgg ccgccaaccg gtcgcgccgg gtggtcgact cgctggtcgc
      541 ccaggactac ctgtactcga ttctcgaact gagcagcgcc ggtgagcggg tcgacgtgat
      601 ggacgtccat cgcgaagcac tggtcgccgc cgagcagacc gcggagctga tggcccggat
      661 cgccagcccc gagatcaaga tcgtcaccct gaccatcagc gagaacggct actgccgcaa
      721 cgcggtcacc ggcgatctgg acatcgacgg cgcactggtc agcgccgacc tggcgaaccc
      781 ggccgtacca cgcaccagca tcggccagtt ggcgggtgcc ctgatccgcc gctaccaggg
      841 cgacgcaggg ccgatcacga tcctgtcctg cgacaacatg gtgtccgccg ggcacaacgc
      901 gcacaagatg gtcagccagt acctgcaggc caccgccaca cccgaaggct tccacgagtg
      961 gttcgccgcg tcggtgacat tcccgaacgc gatggtcgac cgcatcgtgc ccggcaccac
     1021 cgacgccacc cgggcccggg tgcgcgagct gctcggcgtg gtcgacgcga ttccggtgcc
     1081 ggccgagcgg ttcaccatgt gggtgatcga ggacaacttc gccgccggcc gtccggcctg
     1141 ggaggaggcg ggtgcgatct tctccgacga ggtcgaggcc tacgaactgg tcaagctgcg
     1201 gctgctgaac ggctcgcact cgctgatcgc ctacctgggc ggcctgtccg gctccgagac
     1261 gatcccggcc agcttcggcc acgacttcgt ccgtgaatgc gtcaccgaac tgctgaacgt
     1321 cgagtacctg cccagcatcg agctgcccac cggcttcgac ccgcaggcct acatcgcgca
     1381 gctgttcgac cgctggagca atcacgcgct gggcgacgcc accgcccggg tcggctcgga
     1441 cggctcgctc aagctgctgc agcgggtacc cgacccggcc ctgcgcatgc tcgacctcgg
     1501 ccaggtgccc cagcagctcg cgctgatggt ggcgggctgg atcgcctgcg tcgcgccgcc
     1561 caccggattc gatcccggac cgatcgcggc cgccatgatc gaacccgcca aggacaagct
     1621 ggccgcggtc acgtccggtg ccggctccgt ccgcgcgcat gtggaggcca tcctgcgggg
     1681 cggcttcttc cccgacgcgc tggccgccca cgacgagttc gtcggccggg tcgccgagct
     1741 gctgcagatc atcaccaccc acggcgtacg cgacgcggcg aaggccgccc tcgacgcctg
     1801 acccaccgcc caatccgaat cccacaagga gtgtcaatga agaccctgaa gatccacggc
     1861 gccgagagta tcgccatcga ggacgcaccc gtgcccgagc ccggcgaggg cgaggtgcgc
     1921 gtgcgcatgc agtacgccgg tatctgcggc tccgacctgc actactactt ccacggccgc
     1981 aacggcgcct tcgtggtcaa cgaaccgttc acccccggcc acgagatggc gggagtgatc
     2041 gacctcgacc cgtccggacg atgggcgccc ggcactccgg tcaccatcgc gccggccacc
     2101 tacgggcagc cccagcccgg tatcgaggac cgcaagtacc tgtggccggg cggcagttac
     2161 ttcggcagcg cgtccaccac gccgcacacc cagggtggca tgagcgagta ccgcacggtg
     2221 aaggacttca tgatccgcga actgccggac ggattgtcac tgcgcgacgc gtcactggcc
     2281 gagccgctgg cggtcgcact gcatgcgatc cggatcgccg ggggtgttga gggccaaaag
     2341 gtgctggtca ccggctcggg cccgatcggc ctgtgcgtgg tcgcggcatc gtccgcgctg
     2401 ggagctgcgg aagtgaccgc caccgacatg ctggacggcc cgttgcagcg ggccgccgcg
     2461 gtcggtgcga ccgcgaccct gaaggcgggc acggacgagg taccggcttt cgccttcgat
     2521 gtggtgttcg agtgctcggg tgtggcaccc gcggtcaatc aggccctggc cgcggtgcgt
     2581 cgcgcgggca ccgtggtgca ggtgggcatg ctgccggccg acaagatcgc ggtgaacctg
     2641 gagccgctag tgtcgcgcga agtgcgctat ctgggcacct tccggttcga cgacgagatc
     2701 gaccaggcca tcgagttgct cgctgagcat cctgagatcg ccaaggtgat cacgcatgtg
     2761 atcgatgccg accacatcgt cgaggcgttc gagactgccc gcaacagcca ggagtccggc
     2821 aaggtcgtgg tctccctctg gctggacaag gaggcctgac cctggccggc acctgagggt
     2881 gccgccttgg ccagccctcc gtccgggctc caatcccggc tcgggatctt cggcccaggc
     2941 gggtacgtcc aggctccttt accggccgga ggggcccagg ctcttcggtc caggctcgtc
     3001 ggcccagtca catggttcca cggttgccca gcccggtgcc ggtgcgggtg cccaccagtg
     3061 aaccatcgcc acgaggcccg gagccggttc agcgtgcccg cacgcactct gagtcacccg
     3121 acaccacccc caaccaagac cccaccccct gcaacaacgc cgcccccagc aacatccgca
     3181 ccaccgctac accagcaccc ccaccgccgc agcacaccac cacccatcac ccacaaggag
     3241 cattgatgac agcagccaac gctgccggca ccccgtccgg caacgtcgcc cagcgctcga
     3301 ccaaagacct tgccaaggcg gccgtttccg gctggcttgg cacggccctc gaattcatgg
     3361 acttccagct ctactcgctc gccgcggcgc tggtgttcaa ggacatcttc ttccccagcg
     3421 acgcccccgg catggccatc atcttggcca tggccaccta cgccaccggc tacctgagcc
     3481 gtcccctggg cgcctggtac ttcggccggc tcggtgaccg cgtcggccgc accaaggtgc
     3541 tcttctacac gatcgcactg atgggcatcg cgaccaccgc catcggcctg ctgcccacct
     3601 accaccaggt cggcattctt gccccgatcc tgctcgtgtt cctgcgactg gttcagggct
     3661 tcggcgcggg cgccgagatc gccggctctt cggtgatgct cactgagtac gccccccgcc
     3721 gcaagcgtgg tctggtcggc tcgctggtgg cactgggcac caactccggc accctcggcg
     3781 cttcggccat ctggggcgtc ctggtcgcca ccatgtcgac tcagacggtg atcgagtggg
     3841 gttggcgcat ccccttcatc gcgtcctcgc tgatcatgct gttcgccgtg tggctgcgcc
     3901 gcaacctcaa ggagtcgccg gtcttcgaag gacgcgcgga cgtcgtcgac ggcgaagccc
     3961 tctcgcgcga ggaactcgac aaggctgcgg cacacgacaa tgtgctggcc gaggccttgc
     4021 acgaacgcaa gggccgcgcc ttcttcgagg ggttcttcct gcgcttcggc caggccggca
     4081 actcaggaat ggttcagacc tacctgatca gctacatcac cgccgtactg ctgctcgcgc
     4141 cgaccatcgg caccggcgtg gtgatcacct cgtccctggt cggtttcgcc accgtgcccc
     4201 tgatcggttg ggcgagcgac ttcgttggcc gcaaggtgat gtacatcacg atgtcatcca
     4261 tcgcgctggt gctggccgtg ccgatgatct ggatgatcgc caccaagaac gtgagcctcg
     4321 tattcgccgg ctacatcatc cttcaccaga cctcggtact ggctctggct tcgcttgaga
     4381 acaacacgat gtcagagctc ttcggcgccc gggcacggct gacccagctg gccctggcca
     4441 aggagttggc ggccatcgtg gccaccggta tcggtccggt cgcggccgcc gctctggtcg
     4501 tcgccaccgg ttcatggtgg ccgatcgcga tcatgatcgg cttcttctcg atctgctcgc
     4561 tgggcgcggc gatcgtcatg cccgagacgg cgggccgcga cctgaccgtg ctgaacgatg
     4621 ctcgcaaggg cgaggccatc atcggcccgt tcgccaggcg tgagcgcgcc aagctggggc
     4681 tgcccgcacc ggaggaa
//
LOCUS       DAOSUG010000217         4654 bp    DNA     linear   ENV 10-JUN-2024
DEFINITION  MAG TPA_asm: Micropruina sp. isolate AMR_MDS_2195
            NODE_7968_length_4654_cov_3.795173, whole genome shotgun sequence.
ACCESSION   DAOSUG010000217 DAOSUG010000000
VERSION     DAOSUG010000217.1
DBLINK      BioProject: PRJNA925477
            BioSample: SAMN32864262
            Sequence Read Archive: SRR4236673
KEYWORDS    WGS; ENV; Metagenome Assembled Genome; MAG; Third Party Data; TPA;
            TPA:assembly.
SOURCE      Micropruina sp. (wastewater metagenome)
  ORGANISM  Micropruina sp.
            Bacteria; Bacillati; Actinomycetota; Actinomycetes;
            Propionibacteriales; Nocardioidaceae; Micropruina.
REFERENCE   1  (bases 1 to 4654)
  AUTHORS   Abdulkadir,N., Saraiva,J.P., Zhang,J., Stolte,S., Gillor,O.,
            Harms,H. and Rocha,U.
  TITLE     Genome-centric analyses of 165 metagenomes show that mobile genetic
            elements are crucial for the transmission of antimicrobial
            resistance genes to pathogens in activated sludge and wastewater
  JOURNAL   Microbiol Spectr 12 (3), e0291823 (2024)
   PUBMED   38289113
REFERENCE   2  (bases 1 to 4654)
  AUTHORS   Abdulkadir,N.
  TITLE     Direct Submission
  JOURNAL   Submitted (24-MAR-2023) Environmental Microbiology, Helmholtz
            Center for Environmental Research -UFZ, Permoserstr 15, Leipzig
            04318, Germany
COMMENT     The annotation was added by the NCBI Prokaryotic Genome Annotation
            Pipeline (PGAP). Information about PGAP can be found here:
            https://www.ncbi.nlm.nih.gov/genome/annotation_prok/
            
            ##Genome-Assembly-Data-START##
            Assembly Method        :: SPAdes v. 3.11.1
            Genome Representation  :: Full
            Expected Final Version :: Yes
            Genome Coverage        :: Not Applicable
            Sequencing Technology  :: Illumina MiSeq
            ##Genome-Assembly-Data-END##
            
            ##Genome-Annotation-Data-START##
            Annotation Provider               :: NCBI
            Annotation Date                   :: 05/04/2023 04:57:10
            Annotation Pipeline               :: NCBI Prokaryotic Genome
                                                 Annotation Pipeline (PGAP)
            Annotation Method                 :: Best-placed reference protein
                                                 set; GeneMarkS-2+
            Annotation Software revision      :: 6.5
            Features Annotated                :: Gene; CDS; rRNA; tRNA; ncRNA
            Genes (total)                     :: 2,776
            CDSs (total)                      :: 2,750
            Genes (coding)                    :: 2,714
            CDSs (with protein)               :: 2,714
            Genes (RNA)                       :: 26
            tRNAs                             :: 24
            ncRNAs                            :: 2
            Pseudo Genes (total)              :: 36
            CDSs (without protein)            :: 36
            Pseudo Genes (ambiguous residues) :: 0 of 36
            Pseudo Genes (frameshifted)       :: 2 of 36
            Pseudo Genes (incomplete)         :: 32 of 36
            Pseudo Genes (internal stop)      :: 2 of 36
            ##Genome-Annotation-Data-END##
FEATURES             Location/Qualifiers
     source          1..4654
                     /organism="Micropruina sp."
                     /mol_type="genomic DNA"
                     /submitter_seqid="NODE_7968_length_4654_cov_3.795173"
                     /isolate="AMR_MDS_2195"
                     /isolation_source="Activated sludge of municipal
                     wastewater treatment plants"
                     /db_xref="taxon:2737536"
                     /environmental_sample
                     /geo_loc_name="Canada"
                     /lat_lon="49.25 N 123.1 W"
                     /collection_date="2015-06-16"
                     /metagenome_source="wastewater metagenome"
                     /note="metagenomic"
     gene            <1..394
                     /locus_tag="PLF56_08040"
     CDS             <1..394
                     /locus_tag="PLF56_08040"
                     /inference="COORDINATES: protein motif:HMM:NF036831.2"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_function: GO:0005524 - ATP binding [Evidence IEA];
                     GO_function: GO:0016887 - ATP hydrolysis activity
                     [Evidence IEA]"
                     /codon_start=2
                     /transl_table=11
                     /product="MoxR family ATPase"
                     /protein_id="HOQ53550.1"
                     /translation="LDHHGGGNPLADLQPVTDVATVAALADSIRTIFVHGAIKDYLVS
                     VLEATRTSPEVRLGASPRAGLQLLRAARARAMLSGRHYVIPDDIQALAGPALAHRMLL
                     SPAAQLARRNVSDVVASAVASVPLPDRD"
     gene            402..1601
                     /locus_tag="PLF56_08045"
     CDS             402..1601
                     /locus_tag="PLF56_08045"
                     /inference="COORDINATES: protein motif:HMM:NF013998.2"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="DUF58 domain-containing protein"
                     /protein_id="HOQ53551.1"
                     /translation="MIRLTTRGFGLLSTGVVLLIAASATGVRAVAWPGGLLIGLVLAA
                     GGLTWFSARRPTLRRRLLPDRLAAGSPMRVQLDLQRDSIGAGAWSVVEETVPPELTGA
                     PALAVPSGWGGLRSVHEYQLGTTVRGRYRVGPGIWVTTDPLGLASSRRGLGGTSLLTV
                     TPAVHRLGTLGTAAGAGLTGESAQRSSSLLGADDALIREYRPRDELRRIHWPSTARMG
                     SLMVRREEHAWEPSALILLDNRSGAHSGGGSGSSFEWAVSAAASIGVHLLDAGFDVEL
                     TDASGRTLAPEVGASAREVLLDHLTDVHLSNAYELSGALMQGIGGRGNLLIAVLGRLS
                     TADAMALTNARTDGRLCRALILHAPDGPGTDPAELLVADGWQVVRDAASRSVADACAG
                     FGWSARG"
     gene            1598..3769
                     /locus_tag="PLF56_08050"
     CDS             1598..3769
                     /locus_tag="PLF56_08050"
                     /inference="COORDINATES: protein motif:HMM:NF023418.2"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="DUF3488 and transglutaminase-like
                     domain-containing protein"
                     /protein_id="HOQ53552.1"
                     /translation="MRATDRSGVAVMVAMALALFALRPVTSDGRLFVDAVAMIAVVGV
                     GGILSRRLLPTERGARLVQTLAAAAALIGLALVEGVSPNPFAVPELLAGAMRWTVESS
                     APMGPQLGVRMVAVAGVTLLALFADQLTVSLNQPAWSLLPLGLLYLVPALALPALVSF
                     WPVLGLGCGYLLVLLADGVNRSRVLRFQAANVTARGRPRFGGGMLLGGMISLLCALLV
                     AGLAGALTPGLDPSRGAPFTGSGPVQMGDPSLDLRRNLQQPVDRRVITYTTSRDTGSY
                     LRMTSLPGFDETGFHLNAIDLYGGSLPSPEGAAAGRPRFTVDVTVGEFNAEWLPLPYA
                     PASFEASGEWRHDPVSLSVLASGTQQKRATNGLHYTALAMDVTPTAAELAKASAGRPR
                     DAAVTAALPAAFPEQIVQLARQVTAKGTTDGEKAVLLQNWLRGPAFTYSTEPAPGSGY
                     TALTEFLLDDRTGYCEQFAASMAVMARALGIPARVAIGFLPGRKVGDHWEVNIRDMHA
                     WPELYFAGLGWVSFEPTPGVATAPAYTGAAVEQPSASPSASSTPTRSTASSEPSTQPS
                     EAPVEQQSEQAEPVDLSWLGWVGAGLGVVALAAVPSLVRGARRRRRLDVGSVPRQAVG
                     AAWDEVRDTVWDAGREWPHGSPRQIGSEVGRGLPDEAAAAMGRVAVLVERARYADSLG
                     DTTGLHEDVRLVREAVAAGARTARWQRWLPRSLWRGLWCRG"
     gene            complement(3885..4256)
                     /locus_tag="PLF56_08055"
     CDS             complement(3885..4256)
                     /locus_tag="PLF56_08055"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_011756512.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="DUF3040 domain-containing protein"
                     /protein_id="HOQ53553.1"
                     /translation="MALSEDEQRLLDQMEAALAAEDPKLANALRGTTTRRLHRRRAAI
                     AGAGFLIGVACLVGGMQVSPALSIVGFVLMLIASVIAVTSWRHVGDVSSGQSATRSPQ
                     GEKAFMDKMEERWRRRQDEGQ"
     gene            complement(4358..>4654)
                     /locus_tag="PLF56_08060"
     CDS             complement(4358..>4654)
                     /locus_tag="PLF56_08060"
                     /inference="COORDINATES: protein motif:HMM:NF023227.2"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="DNA polymerase IV"
                     /protein_id="HOQ53554.1"
                     /translation="VSKTLALNSPTDSTDELHAQAVTLYRKFGLDRPRIRRVGIRVEG
                     LVNADQAYCQPTLDAPERGWREAELATDAIRRKFGPHCVQRAVLTRNGYASTRA"
BASE COUNT          600 a         1599 c         1744 g          711 t
ORIGIN      
        1 gctcgatcat cacggcggcg gcaacccgct ggccgacctg cagccggtga ccgacgtggc
       61 caccgtcgcg gcgctggccg actcgatccg cacgatcttc gtgcacggtg cgatcaagga
      121 ctatctggtc tcggtgctgg aggccacccg caccagccct gaggtccggc tgggggccag
      181 cccccgcgcc ggcctgcaac tgctacgggc cgcccgcgcg cgggcgatgc tcagcggtcg
      241 ccactacgtc atccccgacg acatccaggc gctcgccggc cccgccctcg cgcatcgcat
      301 gctgctcagc ccggcggcgc agctggccag gcgcaacgtg agcgacgtgg tggcctcggc
      361 ggtggcctcc gttcccctcc ccgaccgcga ctgagacggc cgtgattcgg ctcaccacca
      421 ggggattcgg tctgctgagc accggcgtgg tgctgctgat cgccgcctcg gccacgggcg
      481 tccgggcggt cgcgtggccg ggtggactgt tgatcggtct ggtgctcgcc gcgggcgggc
      541 tgacctggtt ctcggcgcga cggcccacgc tgcggcgccg gctgctgccc gaccggctgg
      601 ccgcaggatc accgatgcgg gtgcaactcg acctgcagcg cgacagcatc ggcgcgggcg
      661 cgtggagcgt ggtggaggaa accgtgcccc ccgagctgac cggggcaccg gccctggccg
      721 tgccgtcggg ctggggcgga ttgcgcagcg tccacgagta tcaactgggc acgacggtcc
      781 ggggccgtta ccgggtcggc cccggcatct gggtgaccac ggatccgctg gggctggcca
      841 gcagccgacg cgggctgggc ggcaccagcc tgctcaccgt cactcctgcg gtgcatcgcc
      901 tcggtacgct cggcacagcg gccggcgcgg ggttgaccgg tgagtcggcc cagcgcagca
      961 gcagcctgct cggggccgac gatgcactga tccgcgagta ccggccacgc gacgagttgc
     1021 gccgcatcca ctggccctcc accgcccgca tgggcagcct gatggtgcgc cgcgaagagc
     1081 acgcgtggga gccgagtgcg ctgatcctgc tcgacaatcg atcgggcgcg cacagcggcg
     1141 gcggctcggg ttcaagcttc gaatgggcgg tctcggcggc ggcgtcgatc ggggtgcacc
     1201 tgctcgacgc gggctttgac gtcgaactga ccgacgcctc cggacgcacc ctggcgcccg
     1261 aggtcggcgc ctcggcccgc gaggtgctgc tggatcacct caccgacgtg cacctcagca
     1321 acgcctacga gctctcgggc gcgctgatgc agggcatcgg tgggcggggc aacctgctga
     1381 tcgccgtgct cggccggctc agcaccgcgg acgccatggc actgaccaat gcccgcaccg
     1441 acggacggct gtgccgcgcg ctgattctgc atgcaccgga cgggccgggc accgacccgg
     1501 ccgagttgct ggtggccgac ggctggcaag tggtccgcga cgcagcctcg cgcagcgtgg
     1561 cggatgcgtg cgcgggtttc gggtggagcg cccgaggatg agagcaacag atcgctccgg
     1621 cgtggcggtg atggtcgcga tggccctggc gctgttcgcg ctgcggccgg tgacctcgga
     1681 cggcaggctc ttcgtggacg ccgtggcgat gatcgccgtg gtcggcgtcg gcggcatcct
     1741 gtctcgccgg ctgctgccca ccgagcgcgg cgctcggctc gtccagaccc tggcggcggc
     1801 ggccgccctg atcggactgg cactggtcga gggcgtctca cccaatccgt tcgccgtgcc
     1861 cgagctgttg gcgggggcga tgcgctggac cgtcgagtcg tccgccccga tggggcctca
     1921 actcggggtg cgtatggtgg ccgtcgctgg ggtcaccctg ctcgcgttgt tcgccgacca
     1981 gctgaccgtg tcgctgaacc agccggcctg gtcgctgctg ccgctgggcc tgctgtacct
     2041 ggtgcctgcg ctggcactgc cggcgctggt gtcgttctgg ccggtgctcg ggctggggtg
     2101 cggctatctg ctggtgctgc tcgccgatgg ggtgaaccgc agccgggtgc tgcggtttca
     2161 ggccgcgaac gtgacggcgc gcgggcgccc acggttcggt ggcgggatgc tgctcggcgg
     2221 catgatcagc ctgctgtgtg cgttgctggt ggccgggctg gccggggcgc tcaccccggg
     2281 cctcgacccg agccgcggcg cgccgttcac cgggtcgggg ccggtgcaga tgggtgatcc
     2341 ctcgctcgac cttcggcgca acctgcagca gccggtggat cgccgggtga tcacctacac
     2401 caccagccgc gacaccggtt cgtacctgcg catgacctcg ctgccgggct tcgacgagac
     2461 cggctttcac ctcaacgcca tcgacctgta cggcggttcg ctcccctcgc ccgaaggtgc
     2521 ggcggcgggg cgtccgcgat tcaccgtcga cgtcaccgtc ggcgagttca acgccgaatg
     2581 gctgccgctg ccgtatgccc cggcgtcgtt cgaggcgtcc ggcgagtggc gccatgatcc
     2641 ggtgtcgctg agcgtgctgg cctcgggcac gcagcagaag cgcgccacca acggcctgca
     2701 ctacacggcc ctcgccatgg acgtgacccc gaccgcggcc gagctggcca aggcgagcgc
     2761 cggacgtccg cgcgacgcgg ccgtgaccgc cgccctgccg gcggcctttc cggagcagat
     2821 cgtgcagctg gcccgtcagg tcaccgccaa ggggaccacg gacggcgaga aggcggtgtt
     2881 gttgcagaac tggttgcgcg gcccggcctt cacctacagc accgagcctg cacccggctc
     2941 gggctacacc gccctcaccg agtttctttt ggacgaccgc accggctact gcgaacagtt
     3001 cgccgcctcg atggcggtga tggcgcgggc gctcggcata ccggcccggg tcgcgatcgg
     3061 ctttctgccc ggacgcaagg tcggtgacca ctgggaggtg aacattcgcg acatgcacgc
     3121 gtggcccgag ctgtacttcg ccgggctcgg ctgggtgagc ttcgagccga cgcccggtgt
     3181 cgccacggcg ccggcctaca ccggcgcggc cgtcgagcag ccctcggcgt ccccgtcggc
     3241 cagctcgaca ccgacccgca gcaccgcgag cagcgagccg agcacgcagc cgtccgaggc
     3301 cccggtcgag cagcagagcg aacaggccga gcccgtggac ctgtcctggc tcggctgggt
     3361 gggcgccggg ctgggagtcg tggcgctggc ggcggtgccg agcctggtgc gcggcgcccg
     3421 gcgacgccgc cggctggacg tgggcagcgt gccgcggcag gcggtgggtg cagcctggga
     3481 cgaggtgcgc gacaccgtat gggacgccgg tcgcgagtgg ccgcacggct caccccggca
     3541 gatcggcagc gaggtgggac gagggctgcc cgatgaggcg gcggcggcga tgggccgggt
     3601 ggcggtgctg gtggagcgcg cgcgctacgc cgacagcctc ggcgacacca caggcctgca
     3661 tgaggacgtg cggctggtcc gcgaggcggt ggccgctggg gcgcggaccg cgcgatggca
     3721 aagatggctg ccacggtcgc tgtggcgagg gttgtggtgt cgcggctgag ggtgctctgg
     3781 tcgacgcacg attcgccggc cgaggttgtc ggataccccc ggtggggaga ttctcgaccg
     3841 gcccggccgg cggttggtga gctgcaggag ggcccgggtg ggccttactg accttcgtcc
     3901 tggcgtcggc gccagcgctc ttccatcttg tccatgaacg ccttctcacc ttgcggcgag
     3961 cgggtggcgg actgaccgga cgagacgtcg ccgacgtggc gccatgaggt gacggcgatc
     4021 acgctggcga tcagcatgag cacgaagccg acgatgctga gggccgggct cacctgcatg
     4081 ccgccgacca ggcaggcgac accgatcaga aagccggcac cggcgatcgc ggcgcgccgt
     4141 cggtgaagcc gacgggtggt ggtgccccgt agcgcgttgg ccagtttggg atcttcggcc
     4201 gccagggctg cctccatctg gtcgaggagt cgctgttcgt cctctgaaag tgccatgtca
     4261 acctcccttc ggttcggggg cgcaggtcga gcgcggctca agtctaggcg ggcggatcgc
     4321 caaacggaac accagtgcgg gtggatgaac gtgaggttca ggcccgggtg gacgcatacc
     4381 cgttacgggt cagcaccgca cgctgcacac agtgcgggcc gaacttgcgc cgaatggcgt
     4441 ccgtggccag ttcggcctca cgccagcccc gttcgggagc gtccaaagtg ggctggcagt
     4501 aggcctgatc ggcgttcacc aggccctcga ctcgaatgcc gacccggcga atacggggac
     4561 ggtcgaggcc gaactttcga tacagcgtca cggcctgcgc atggagttcg tcggtggagt
     4621 cggtggggct gttcagggcg agggttttcg acac
//
LOCUS       DAOSUG010000218         4638 bp    DNA     linear   ENV 10-JUN-2024
DEFINITION  MAG TPA_asm: Micropruina sp. isolate AMR_MDS_2195
            NODE_8003_length_4638_cov_4.274711, whole genome shotgun sequence.
ACCESSION   DAOSUG010000218 DAOSUG010000000
VERSION     DAOSUG010000218.1
DBLINK      BioProject: PRJNA925477
            BioSample: SAMN32864262
            Sequence Read Archive: SRR4236673
KEYWORDS    WGS; ENV; Metagenome Assembled Genome; MAG; Third Party Data; TPA;
            TPA:assembly.
SOURCE      Micropruina sp. (wastewater metagenome)
  ORGANISM  Micropruina sp.
            Bacteria; Bacillati; Actinomycetota; Actinomycetes;
            Propionibacteriales; Nocardioidaceae; Micropruina.
REFERENCE   1  (bases 1 to 4638)
  AUTHORS   Abdulkadir,N., Saraiva,J.P., Zhang,J., Stolte,S., Gillor,O.,
            Harms,H. and Rocha,U.
  TITLE     Genome-centric analyses of 165 metagenomes show that mobile genetic
            elements are crucial for the transmission of antimicrobial
            resistance genes to pathogens in activated sludge and wastewater
  JOURNAL   Microbiol Spectr 12 (3), e0291823 (2024)
   PUBMED   38289113
REFERENCE   2  (bases 1 to 4638)
  AUTHORS   Abdulkadir,N.
  TITLE     Direct Submission
  JOURNAL   Submitted (24-MAR-2023) Environmental Microbiology, Helmholtz
            Center for Environmental Research -UFZ, Permoserstr 15, Leipzig
            04318, Germany
COMMENT     The annotation was added by the NCBI Prokaryotic Genome Annotation
            Pipeline (PGAP). Information about PGAP can be found here:
            https://www.ncbi.nlm.nih.gov/genome/annotation_prok/
            
            ##Genome-Assembly-Data-START##
            Assembly Method        :: SPAdes v. 3.11.1
            Genome Representation  :: Full
            Expected Final Version :: Yes
            Genome Coverage        :: Not Applicable
            Sequencing Technology  :: Illumina MiSeq
            ##Genome-Assembly-Data-END##
            
            ##Genome-Annotation-Data-START##
            Annotation Provider               :: NCBI
            Annotation Date                   :: 05/04/2023 04:57:10
            Annotation Pipeline               :: NCBI Prokaryotic Genome
                                                 Annotation Pipeline (PGAP)
            Annotation Method                 :: Best-placed reference protein
                                                 set; GeneMarkS-2+
            Annotation Software revision      :: 6.5
            Features Annotated                :: Gene; CDS; rRNA; tRNA; ncRNA
            Genes (total)                     :: 2,776
            CDSs (total)                      :: 2,750
            Genes (coding)                    :: 2,714
            CDSs (with protein)               :: 2,714
            Genes (RNA)                       :: 26
            tRNAs                             :: 24
            ncRNAs                            :: 2
            Pseudo Genes (total)              :: 36
            CDSs (without protein)            :: 36
            Pseudo Genes (ambiguous residues) :: 0 of 36
            Pseudo Genes (frameshifted)       :: 2 of 36
            Pseudo Genes (incomplete)         :: 32 of 36
            Pseudo Genes (internal stop)      :: 2 of 36
            ##Genome-Annotation-Data-END##
FEATURES             Location/Qualifiers
     source          1..4638
                     /organism="Micropruina sp."
                     /mol_type="genomic DNA"
                     /submitter_seqid="NODE_8003_length_4638_cov_4.274711"
                     /isolate="AMR_MDS_2195"
                     /isolation_source="Activated sludge of municipal
                     wastewater treatment plants"
                     /db_xref="taxon:2737536"
                     /environmental_sample
                     /geo_loc_name="Canada"
                     /lat_lon="49.25 N 123.1 W"
                     /collection_date="2015-06-16"
                     /metagenome_source="wastewater metagenome"
                     /note="metagenomic"
     gene            <1..1001
                     /locus_tag="PLF56_08065"
     CDS             <1..1001
                     /locus_tag="PLF56_08065"
                     /inference="COORDINATES: protein motif:HMM:NF040070.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=3
                     /transl_table=11
                     /product="DUF6350 family protein"
                     /protein_id="HOQ53555.1"
                     /translation="LCLVLARSLVALVLRAAPAVALEGRGALRAWGLSSAGYAFVACV
                     IALASGAAPRVGWALLGGALVGGLGAGWALRRRLLALRPLPRWLVGVPQAILGGVATL
                     TAISAAVLLVALIVGGERVGQLENALAPDGVGSWLLVIAQLLFLPNLLAWTASWVLGA
                     GFSVGVGSFVSPMLTSVGLLPAIPVFGAVPQPGGGNAWAYAWLLSGLLTGAVAGVVSS
                     ARPAVGGTLVRALARGAGSGLGTALAVVLIGVLSRGDLGSGRLVGLGPVLLNLIWLAP
                     LPMVVGGTLAALVHWFARARHLPAERKPSGADDTTELFDLPTEPLEQPTVPLGRRG"
     gene            1172..1786
                     /gene="purN"
                     /locus_tag="PLF56_08070"
     CDS             1172..1786
                     /gene="purN"
                     /locus_tag="PLF56_08070"
                     /EC_number="2.1.2.2"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_017934641.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_function: GO:0004644 - phosphoribosylglycinamide
                     formyltransferase activity [Evidence IEA];
                     GO_process: GO:0009152 - purine ribonucleotide
                     biosynthetic process [Evidence IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="phosphoribosylglycinamide formyltransferase"
                     /protein_id="HOQ53556.1"
                     /translation="MAYRIAVLVSGTGSLLQALLDAEACGELQAQVVVVGADTAGAQG
                     LGRAEKAGVATFVHPYRKGDDRLAWDEALTGLVAAHEVDLVVLAGFMKLVGPAFLARF
                     GGRTINTHPALLPSFPGMHGPRDALVHGVKITGTTVFEVDAGVDTGRILAQQAVPVLP
                     GDTVESLHERIKVAERALLVETVNELADEHLTAASHPTASQGDS"
     gene            1783..3330
                     /gene="purH"
                     /locus_tag="PLF56_08075"
     CDS             1783..3330
                     /gene="purH"
                     /locus_tag="PLF56_08075"
                     /EC_number="2.1.2.3"
                     /EC_number="3.5.4.10"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_014847099.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_function: GO:0003824 - catalytic activity [Evidence
                     IEA];
                     GO_function: GO:0003937 - IMP cyclohydrolase activity
                     [Evidence IEA];
                     GO_function: GO:0004643 -
                     phosphoribosylaminoimidazolecarboxamide formyltransferase
                     activity [Evidence IEA];
                     GO_process: GO:0006164 - purine nucleotide biosynthetic
                     process [Evidence IEA];
                     GO_process: GO:0006189 - 'de novo' IMP biosynthetic
                     process [Evidence IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="bifunctional
                     phosphoribosylaminoimidazolecarboxamide
                     formyltransferase/IMP cyclohydrolase"
                     /protein_id="HOQ53557.1"
                     /translation="MTDRLPLRRALLSVYDKTGLVELGRALADAGVELVSTGSTARTL
                     TDAGIAVIPVEQVTGFAECLDGRVKTLHPHIHAGLLADLRLESHRDQLAELGIEPFQL
                     VVSNLYPFSATVASGASPDECVEQIDIGGPSMVRAAAKNHPSVAIVTSPEQYPCVVDA
                     LAAGGFTLGERQGLATVAFRHTAAYDIAVAGWMGEQFHAADFPAWIAGTWHKVGALRY
                     GENSHQAAALYRYPNGPAGITAAEQLHGKEMSYNNYTDGDAAYRAAYDFTEPAVAIIK
                     HANPCGIAVGADVAHAHRLAHACDPVSAFGGVIATNRPVSVAMAEQVAEVFTEVLIAP
                     DYEPGALEILSRKKNVRLLKAAPYEHRERQLQPISGGALLQQPDRIDAPGDDPVNWTL
                     AAGQPADEATLADLAFAWRACRAVKSNAILLAAGGASVGVGMGQVNRVDSCRLAVERA
                     GERAAGSVAASDAFFPFADGPGILIAAGVRAIVQPGGSMRDAETIEAAQAAGVTMYFT
                     GTRHFFH"
     gene            3361..3663
                     /locus_tag="PLF56_08080"
     CDS             3361..3663
                     /locus_tag="PLF56_08080"
                     /inference="COORDINATES: protein motif:HMM:NF022668.2"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="DUF3017 domain-containing protein"
                     /protein_id="HOQ53558.1"
                     /translation="MTAPAERPPKSFAQQVWGQWPLASVLLGAFAGLAIAATGHWRTG
                     TTLIGLSITMGGLFRLLPQRRVGLLAVRNRPVDTLVLLIIGVGITVLSWAVPALRP"
     gene            complement(4074..>4638)
                     /locus_tag="PLF56_08085"
     CDS             complement(4074..>4638)
                     /locus_tag="PLF56_08085"
                     /inference="COORDINATES: ab initio
                     prediction:GeneMarkS-2+"
                     /note="Derived by automated computational analysis using
                     gene prediction method: GeneMarkS-2+."
                     /codon_start=2
                     /transl_table=11
                     /product="hypothetical protein"
                     /protein_id="HOQ53559.1"
                     /translation="LAVFVLLVLMLPFAQSAAADRIWPIQRANPGLSEEVVRSLPEIV
                     KNSNVKVAPDFVAGTPIAYPNGELVPGQSAKTVAAALACTEQVYSAASGYWGPVAISC
                     TSIFGSPGYEVVYKWQATTGDVATKGVVRVRGFSSGTTEYWKSNGQNIGYETRFITVT
                     WGNVLANLKAQAYKVSGGFAFTAHFWH"
BASE COUNT          644 a         1587 c         1646 g          761 t
ORIGIN      
        1 ccctgtgcct ggtgctggct cgctcgctgg tcgctctggt gctgcgcgcc gcccccgccg
       61 tcgcactcga gggacggggc gcgctgcgcg cgtggggcct gtcgagcgcc ggctacgcct
      121 tcgtggcctg cgtcatcgcg ttggcctcgg gtgcggcacc cagggtcggc tgggcgttgc
      181 tcggtggtgc actggtgggt ggcctcggcg cgggatgggc cctgcgccgc cggttgttgg
      241 cgctgcgccc gctgccacgg tggctggtcg gcgtgccgca ggcgattctc ggcggcgtgg
      301 ccacactgac cgcgatctcg gccgcggtgc tgctggtggc cctgatcgtc ggcggtgaac
      361 gggtggggca gctcgagaac gccctggcac cggacggtgt cggctcgtgg ttgctggtca
      421 tcgcccaact gctcttcctg cccaatctgc tggcctggac ggccagctgg gtgctcggcg
      481 ccgggttcag cgtaggggtc gggtcgttcg tctcgccgat gctgaccagc gtcggcctgc
      541 tgcccgccat tccggtcttc ggcgccgtgc cgcaaccggg cggcggcaac gcgtgggcct
      601 atgcctggtt gctcagcgga ctgctcaccg gcgcggtcgc aggcgtggtg agctcggcgc
      661 gtccggcggt gggtggcaca ctcgtgcggg ccctggcccg cggggcgggc tcggggctgg
      721 gcacggcact cgcggtggta ctcatcggtg tgctgtcccg gggcgatctg ggatccggac
      781 ggctggtggg cctcggcccg gtgctgctga acctgatctg gctggcgccg ctgccgatgg
      841 tggtgggcgg cacgctcgcg gcgctcgtcc actggttcgc cagggcacga cacctgcccg
      901 cggagcggaa gccgtcgggc gcggatgaca ccaccgagtt gttcgacctg ccgaccgaac
      961 ccctcgagca gccaacggtt ccgctgggcc ggcgcggctg agcacacgcg catccgctcc
     1021 gcagccaacc gaacccggcg acgtccagag cgtcggtggg cacgagcgct ggctctgggc
     1081 ggggccgtcc gatcagccga gccaaggcga ggcgctgtcc gagcctcacg tagcggacga
     1141 gggccggtgg ccggggcact aggctgccgc catggcctac cgcatcgccg tgctcgtctc
     1201 gggcaccggc agcctgctgc aggccctgct ggacgccgag gcctgtggtg agttgcaggc
     1261 ccaggtggtc gtcgtgggcg ccgacacggc cggcgcgcag ggcctgggcc gggccgaaaa
     1321 ggccggggtc gccaccttcg tgcatcctta ccgcaagggc gacgatcggc tcgcctggga
     1381 cgaggccctg accggcctgg tcgcggcgca tgaggtcgac ctggtcgtgc tggccggctt
     1441 catgaagctg gtcgggcccg catttctggc ccgcttcggt ggccgcacga tcaacaccca
     1501 tcccgcgctg ctgcccagct ttcccggcat gcacggccca cgcgacgcgc tggttcatgg
     1561 ggtcaagatc accggtacca cggtcttcga ggtggatgcc ggggtcgata ccggacgcat
     1621 cctggcccag caggccgttc cggtgctgcc cggcgacacc gtcgaaagcc tgcacgagcg
     1681 catcaaggtg gccgagcggg cgctgctcgt cgagaccgtc aacgaactgg ccgacgagca
     1741 cctgacggcc gcctcacacc caaccgcctc ccagggggat tcatgaccga ccggctacca
     1801 cttcgacgcg ccctgctgtc ggtgtacgac aagaccgggc tggtcgaact cggccgcgcc
     1861 ctcgccgacg ccggggtcga actggtctcg accgggtcga cggcgcgcac gttgaccgac
     1921 gccggcattg cggtcatccc ggtcgagcag gtcaccggct tcgccgaatg cctggacggg
     1981 cgggtcaaga cgctgcaccc gcacattcac gccggcctgc tcgctgacct tcggctcgaa
     2041 tcgcaccgcg accaactcgc cgagctgggc atcgagccgt tccagctcgt ggtcagcaac
     2101 ctctacccct tcagcgcgac cgtcgcctcg ggcgcttccc ccgacgagtg cgtcgagcag
     2161 atcgacatcg gcggcccgag catggtgcgg gcggccgcga agaaccaccc gtccgtcgcc
     2221 atcgtcacct cacctgagca gtacccctgc gtggtggacg ccctggccgc cggcggtttc
     2281 accctgggcg agcggcaggg gctggcgacg gtcgcgttcc ggcacaccgc cgcctacgac
     2341 atcgcggtgg ccgggtggat gggcgaacag ttccacgctg ccgactttcc cgcctggatc
     2401 gccggcacct ggcacaaggt cggcgcgctg cgctacggcg agaactcgca ccaggcggcg
     2461 gcgttgtacc gctaccccaa cggtccggcc ggcatcaccg ccgccgagca actgcacggc
     2521 aaagagatga gctacaacaa ctacaccgac ggcgacgcgg cctaccgggc cgcctacgac
     2581 ttcaccgagc cggcggtggc gatcatcaag cacgccaatc cgtgcggcat cgcagtgggc
     2641 gccgacgtcg cgcacgcgca tcggctggcg catgcctgcg acccggtcag cgccttcggt
     2701 ggcgtgatcg ccaccaaccg tccggtgtcg gtggcgatgg ccgagcaggt ggccgaggtg
     2761 ttcaccgagg tgctgatcgc acctgactac gagcccggcg cgctcgagat tctgtctcgc
     2821 aagaagaacg tccggctgct caaggccgcg ccctacgagc atcgggagcg tcagctgcag
     2881 ccgatctcgg gcggcgccct gctgcagcaa cccgaccgca tcgatgcccc cggcgacgac
     2941 ccggtcaact ggacgctcgc ggccgggcag cccgcggacg aggccacgct ggccgacctg
     3001 gcgttcgcct ggcgggcctg ccgggcggtg aagagcaacg ccatcctgct ggccgccggc
     3061 ggagcgtccg tcggagtggg catgggccag gtcaaccggg tcgactcgtg ccgcctcgcc
     3121 gtcgaacggg cgggcgagcg ggccgccggt tcggtggccg cctccgacgc cttcttcccc
     3181 ttcgcggacg gccccggcat cctgatcgcc gcgggggtgc gggcgatcgt ccagcccggc
     3241 gggtcgatgc gcgacgcgga gacgatcgag gcggcgcagg ccgccggggt gaccatgtac
     3301 ttcaccggca cccggcactt cttccactga ggcagcacgg cacgtgacac catcgacccc
     3361 atgaccgcac ccgccgagcg tccgcccaag agcttcgcgc agcaggtgtg ggggcagtgg
     3421 ccgttggcca gcgtgctgct cggcgccttc gccgggttgg cgatcgccgc caccggtcat
     3481 tggcgcaccg gcaccacact gatcggcctc tcgatcacga tgggcggcct gttccgcctg
     3541 ttgccgcaac gacgggtcgg gctgctggcg gtgcgcaatc gtccggtcga caccctcgtg
     3601 ctgctgatca tcggcgtcgg catcacggtg ctgtcgtggg cggttcctgc cctgcgcccc
     3661 tgatcctggt cgcgaggcgg ggtcaacggg acccgctgat cgaagcacga ttcgttgact
     3721 tatctggagg tggctccacg cgcttgatga ggcgtctata cggccaagcg gaacagtgtt
     3781 cggtcattcg cagtcttgtc agtagctcga cgtggtcggt gcgtggggct ggatgggcta
     3841 ttgaggtgcc gtccggtgcg ggtggaggga gcgcttcggc gtgagtctgc cggccaggag
     3901 actttgtcgg gccgtggggc gtggaggtgt gttggagggg ttggactccg aattgtttga
     3961 gtccgaagtc cagccttctt cgtgcaatga gcagcgcggc agctgcccgc aatctcatgg
     4021 gatgcctcag ccatgtcgca actagcgagc taaccggcta gacccacgca aatctagtgc
     4081 cagaagtggg ctgtgaaggc gaacccgcct gagaccttgt aggcctgagc tttcaggttg
     4141 gccagcacat tgccccatgt gaccgtgatg aaccgggtct cgtagccgat gttctggccg
     4201 tttgacttcc agtactcggt cgtgcctgaa gagaagccgc gcacccgcac gacgcccttg
     4261 gtcgcgacat cgccggtggt ggcctgccac ttgtatacga cctcgtagcc ggggctgccg
     4321 aagatgctgg tgcagctgat cgcaacggga ccccagtagc cggaagcggc cgagtagacc
     4381 tgttcagtgc aggccagtgc ggcggcgaca gtcttggcgc tctgcccggg aacgagttct
     4441 ccgttggggt aagcaatcgg cgttccagcg acgaagtcgg gcgcaacctt cacattgctg
     4501 ttcttcacga tctccggcag cgagcggacg acttcctccg agagtcccgg gttggcgcgt
     4561 tggattggcc agatcctatc ggccgcggcg gactgcgcaa acggcagcat cagcactaag
     4621 agcacaaata ccgcaagc
//
LOCUS       DAOSUG010000219         4621 bp    DNA     linear   ENV 10-JUN-2024
DEFINITION  MAG TPA_asm: Micropruina sp. isolate AMR_MDS_2195
            NODE_8039_length_4621_cov_4.352168, whole genome shotgun sequence.
ACCESSION   DAOSUG010000219 DAOSUG010000000
VERSION     DAOSUG010000219.1
DBLINK      BioProject: PRJNA925477
            BioSample: SAMN32864262
            Sequence Read Archive: SRR4236673
KEYWORDS    WGS; ENV; Metagenome Assembled Genome; MAG; Third Party Data; TPA;
            TPA:assembly.
SOURCE      Micropruina sp. (wastewater metagenome)
  ORGANISM  Micropruina sp.
            Bacteria; Bacillati; Actinomycetota; Actinomycetes;
            Propionibacteriales; Nocardioidaceae; Micropruina.
REFERENCE   1  (bases 1 to 4621)
  AUTHORS   Abdulkadir,N., Saraiva,J.P., Zhang,J., Stolte,S., Gillor,O.,
            Harms,H. and Rocha,U.
  TITLE     Genome-centric analyses of 165 metagenomes show that mobile genetic
            elements are crucial for the transmission of antimicrobial
            resistance genes to pathogens in activated sludge and wastewater
  JOURNAL   Microbiol Spectr 12 (3), e0291823 (2024)
   PUBMED   38289113
REFERENCE   2  (bases 1 to 4621)
  AUTHORS   Abdulkadir,N.
  TITLE     Direct Submission
  JOURNAL   Submitted (24-MAR-2023) Environmental Microbiology, Helmholtz
            Center for Environmental Research -UFZ, Permoserstr 15, Leipzig
            04318, Germany
COMMENT     The annotation was added by the NCBI Prokaryotic Genome Annotation
            Pipeline (PGAP). Information about PGAP can be found here:
            https://www.ncbi.nlm.nih.gov/genome/annotation_prok/
            
            ##Genome-Assembly-Data-START##
            Assembly Method        :: SPAdes v. 3.11.1
            Genome Representation  :: Full
            Expected Final Version :: Yes
            Genome Coverage        :: Not Applicable
            Sequencing Technology  :: Illumina MiSeq
            ##Genome-Assembly-Data-END##
            
            ##Genome-Annotation-Data-START##
            Annotation Provider               :: NCBI
            Annotation Date                   :: 05/04/2023 04:57:10
            Annotation Pipeline               :: NCBI Prokaryotic Genome
                                                 Annotation Pipeline (PGAP)
            Annotation Method                 :: Best-placed reference protein
                                                 set; GeneMarkS-2+
            Annotation Software revision      :: 6.5
            Features Annotated                :: Gene; CDS; rRNA; tRNA; ncRNA
            Genes (total)                     :: 2,776
            CDSs (total)                      :: 2,750
            Genes (coding)                    :: 2,714
            CDSs (with protein)               :: 2,714
            Genes (RNA)                       :: 26
            tRNAs                             :: 24
            ncRNAs                            :: 2
            Pseudo Genes (total)              :: 36
            CDSs (without protein)            :: 36
            Pseudo Genes (ambiguous residues) :: 0 of 36
            Pseudo Genes (frameshifted)       :: 2 of 36
            Pseudo Genes (incomplete)         :: 32 of 36
            Pseudo Genes (internal stop)      :: 2 of 36
            ##Genome-Annotation-Data-END##
FEATURES             Location/Qualifiers
     source          1..4621
                     /organism="Micropruina sp."
                     /mol_type="genomic DNA"
                     /submitter_seqid="NODE_8039_length_4621_cov_4.352168"
                     /isolate="AMR_MDS_2195"
                     /isolation_source="Activated sludge of municipal
                     wastewater treatment plants"
                     /db_xref="taxon:2737536"
                     /environmental_sample
                     /geo_loc_name="Canada"
                     /lat_lon="49.25 N 123.1 W"
                     /collection_date="2015-06-16"
                     /metagenome_source="wastewater metagenome"
                     /note="metagenomic"
     gene            complement(<1..495)
                     /locus_tag="PLF56_08090"
     CDS             complement(<1..495)
                     /locus_tag="PLF56_08090"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_011756737.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_function: GO:0003677 - DNA binding [Evidence IEA];
                     GO_function: GO:0008168 - methyltransferase activity
                     [Evidence IEA];
                     GO_function: GO:0008270 - zinc ion binding [Evidence IEA];
                     GO_process: GO:0006281 - DNA repair [Evidence IEA];
                     GO_process: GO:0006355 - regulation of DNA-templated
                     transcription [Evidence IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="Ada metal-binding domain-containing protein"
                     /protein_id="HOQ53560.1"
                     /translation="MIPSREVCLRAVASRDARFDGEFFTAVRTTRIYCRPSCPALTPK
                     PQNTAFYPSAAAAQEAGFRACRRCRPDTVPGSAAWNLRADTAARAMRLIGDGLVDREG
                     VPGVAGRLGYSMRQLQRLLADELGASPLALARAQRAHTARILLETTTMPLGEVAYAAG
                     FGSVR"
     gene            complement(752..1426)
                     /locus_tag="PLF56_08095"
     CDS             complement(752..1426)
                     /locus_tag="PLF56_08095"
                     /inference="COORDINATES: protein motif:HMM:NF024701.2"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="TetR/AcrR family transcriptional regulator"
                     /protein_id="HOQ53561.1"
                     /translation="MDEASPARPYHHGNLRSALVEAGYALAVDEGPQALTLRGATRRA
                     GVSPTAAYRHFADLDELRTAVGFRALQAMAVAIEQHQHKVTALAGLPRRRELLEAVGT
                     GYIAFALDHPNAFRVGLFGLLGMQHAELPAGAGQTGRTPFQLLTDALTGLAAVGALER
                     DSVSSAAIHCWSSVHGFASLAILGPLRSQPRAVQDDMARRLVRDVVDGVLGAHAVRNR
                     LGAWRV"
     gene            1540..2037
                     /locus_tag="PLF56_08100"
     CDS             1540..2037
                     /locus_tag="PLF56_08100"
                     /inference="COORDINATES: protein motif:HMM:NF016840.2"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="DinB family protein"
                     /protein_id="HOQ53562.1"
                     /translation="MTKPDERDLLLGRLRGQRRHVLDQLEGLSDSALRTPVLPSGWTP
                     LGLVRHLTLSDERYWFEVVMAGDALDFWPAGDNADWRVGPVEKAAAVIEAYRTAASAS
                     DAVIEAFTLDAPPRSPEPWWADAGLSFPDLRAVLVHVLVETATHAGHLDAARELIDGR
                     QYLVL"
     gene            2078..2503
                     /locus_tag="PLF56_08105"
     CDS             2078..2503
                     /locus_tag="PLF56_08105"
                     /inference="COORDINATES: protein motif:HMM:NF018663.2"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_function: GO:0046872 - metal ion binding [Evidence
                     IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="VOC family protein"
                     /protein_id="HOQ53563.1"
                     /translation="MATLSPYLNFAGNGREAMSYYHSIFGGDLVLLGPAEFGVTDMPE
                     DSLMHGALTGDGWSILASDAMPGAEQTWGGTRVYCAFFGDDLATQTAWFDALAADGSV
                     GMPLEKQVWGDIYGLVKDKYGIEWMFNVSAPEGWSQQQG"
     gene            complement(2621..3088)
                     /locus_tag="PLF56_08110"
     CDS             complement(2621..3088)
                     /locus_tag="PLF56_08110"
                     /inference="COORDINATES: protein motif:HMM:NF020022.2"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_function: GO:0005524 - ATP binding [Evidence IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="ATP-binding protein"
                     /protein_id="HOQ53564.1"
                     /translation="MALVILLCGPAGAGKTTHARQLAAGGAVRLSMDEAVWDDGWHQD
                     EPPAERLQHLYADLKHDARTAIANGQDVVVDLSSADRGVRDEWRAFADDVSADLRLVV
                     LTAPFEVLWQRVKKRNDQKQANAVRFTESRLRAYVDGFDWPTDDEHPTVVTTG"
     gene            complement(3446..>4621)
                     /gene="ligD"
                     /locus_tag="PLF56_08115"
     CDS             complement(3446..>4621)
                     /gene="ligD"
                     /locus_tag="PLF56_08115"
                     /inference="COORDINATES: protein motif:HMM:TIGR02779.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_function: GO:0003910 - DNA ligase (ATP) activity
                     [Evidence IEA];
                     GO_function: GO:0005524 - ATP binding [Evidence IEA];
                     GO_process: GO:0006281 - DNA repair [Evidence IEA];
                     GO_process: GO:0006310 - DNA recombination [Evidence IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="non-homologous end-joining DNA ligase"
                     /protein_id="HOQ53565.1"
                     /translation="PRGEYGGGSVRIWDAGTFELEKWRDGKEVIATLTGRPGGGLGGV
                     PRRFALLHTKLGGDPKNWLIHLMAPRDGADEQTRPEFEVPSFAPMLATAGTPADVRDG
                     DWVFEVKWDGYRAIAAVVGGTVTLTSRRGLDLTRDFPELAELGRLLDGQQAVLDGEAV
                     LLDAQGRSSFERLQNRSQYPGEAYYMVFDLLYLDGRSLIREPYTARRQKLAELLGEGT
                     RRVQLGTQLGTDADEALAISAELGLEGVVAKRPDSVYQPGRRAHTWVKIKHVLTQEVV
                     IIGWTPGESARARTIGALLMAVPNDAGLTYVGRVGSGFTEKDLVDTRQVLGEIEVPAA
                     PVSDVPRADAKGAHWVEPILVGEVGYGVWTNAGRLRMPVWRGWRPDKTPEDISLPQD"
BASE COUNT          707 a         1644 c         1559 g          711 t
ORIGIN      
        1 acggacggag ccgaaccccg cggcgtaggc gacctcaccc agcggcatcg tggtcgtttc
       61 aagcagaatc cgggccgtgt gcgcccgctg cgcgcgggcc agcgccaacg gggaggcacc
      121 cagttcgtct gccagcagac gctgcagttg ccgcatcgaa tacccgagac ggccggcgac
      181 ccccggcacc ccctcccgat cgaccagccc gtcgccgatc agccgcatgg cgcgagcggc
      241 ggtgtcggcc cgcaggttcc aggcggccga accgggcacg gtgtcgggac ggcacctccg
      301 gcaggcgcga aaaccggcct cctgcgcggc agcggcgctg gggtagaagg ccgtgttctg
      361 cggcttcggc gtgagcgccg gacagctcgg ccggcagtag atgcgggtgg tgcgcacggc
      421 ggtgaagaac tcgccgtcaa agcgcgcgtc gcggctggcg acggctcgca ggcacacctc
      481 gcggctgggg atcatgcgtt ccattctgga cgcccgaggg gctgaaacct agcaagaatg
      541 cgacatggca gttgcttgtg cagcgacagt tcgctgcgag ggcaccccgc tgggatccgc
      601 gtgcgccgtg cccgtatcgg ggcccggcag tctgccatcg gctctcgcga cgcgacatgc
      661 ccccagactg gcccacgccg ggtcacagcc gggtgccaac cggaggcact gggcgttcag
      721 cagcggagcc cactaccctc caccgccgat gtcagacccg ccaagcgccc agccgattgc
      781 gcaccgcgtg cgcacccagc accccgtcca ccacgtcgcg gacgagtcga cgcgccatat
      841 cgtcctgtac cgcgcgaggc tggctgcgga gcggcccgag gatcgccagc gaggcgaagc
      901 cgtgcacgct cgaccagcaa tgaatggctg cggacgacac ggagtcgcgc tcgagtgccc
      961 cgaccgcggc cagcccggtc agggcgtcgg tgagcagttg gaaaggcgtg cggccggtct
     1021 ggccggcacc cgcgggcagt tcggcgtgct gcatgccgag caggccgaaa agcccgaccc
     1081 ggaaggcgtt ggggtggtcg agggcgaagg cgatgtagcc ggtgccgacg gcctccagca
     1141 actccctgcg cctgggcaga ccggcgagcg cggtgacttt gtgctgatgc tgttcgatgg
     1201 cgacggccat tgcctgcagg gcacggaacc cgaccgcggt gcgcaactcg tccaggtcgg
     1261 cgaagtgccg gtacgcggcc gtcgggctga cccccgcccg ccgggtggcg ccacgcagcg
     1321 tgagggcctg cggcccctcg tcgacggcca gggcgtaccc cgcctccacc agtgccgagc
     1381 gcaggttgcc gtgatggtac ggacgcgcgg ggcttgcctc gtccatggca gcactctact
     1441 ctgatcccac gcaatgttca cattgtcaac ttgagggagt gtcgtgttcc tggcgtcggt
     1501 tgaaccgaat ctgtcggcgg ctggtgccaa ggtgcgtgca tgacgaaacc ggacgaacgt
     1561 gacctgctgc tcggccgcct gcgcggccag cgacggcacg ttctcgacca actcgaaggg
     1621 ctgagcgact ccgcgctgcg cactccggtt ctgccgtccg ggtggacgcc gctcggactc
     1681 gtgcgtcatc tcaccctctc ggacgagcgg tactggttcg aggtggtgat ggccggcgat
     1741 gcgctcgact tctggccggc cggtgacaat gccgactggc gggtcggccc cgtcgaaaag
     1801 gcggcggcgg tgatcgaggc ctatcggacg gccgccagcg cgtccgatgc ggtgatcgaa
     1861 gccttcacgc tcgatgcgcc gccccgcagc cccgagccgt ggtgggccga cgccgggctc
     1921 agctttcccg acctgcgggc cgtgctcgtg cacgtgctgg tcgagaccgc cacccacgcg
     1981 gggcacctcg atgcggcccg tgaactcatc gacggcaggc agtacctcgt gctgtaacca
     2041 gttccccatc catcatccga ccgacaagga cgaatccatg gcaacactca gtccctacct
     2101 caacttcgcg ggcaatggcc gtgaggcgat gagctattac cacagcatct tcggcggtga
     2161 cctcgtgctg ctggggccgg ccgagttcgg cgtcacggac atgcctgaag acagcctgat
     2221 gcacggcgcg ctgaccggcg acggctggtc catcctggct tcggacgcca tgcctggggc
     2281 tgagcagacc tggggcggca cccgcgtgta ctgcgccttc ttcggcgacg acctcgccac
     2341 tcaaaccgca tggttcgatg ccctggcggc agacggctcc gtcggcatgc cgctcgagaa
     2401 gcaggtgtgg ggcgacatct acggcctcgt caaagacaag tacggcatcg agtggatgtt
     2461 caacgtctcg gcacccgagg gctggtcgca gcagcagggc tgacggatcg gcaccgacta
     2521 ctggtcgcag cagcagaact gacgtctgaa ggccgctcgt tgtcttcgcc acctcgtggc
     2581 gcagccgggc ccagccgggc tttgccagcc ttagccgggc ttagccggtc gtgaccaccg
     2641 tcgggtgctc gtcatccgtc ggccagtcga agccgtccac gtaggcgcgc agtcgcgact
     2701 cggtgaaccg cacggcgttg gcctgtttct ggtcgttgcg cttcttcacc cgctgccaca
     2761 gcacctcgaa cggtgcggtc agcacgacca gccgcaggtc cgccgacacg tcgtcggcga
     2821 acgcacgcca ctcgtcacgc acgccgcgat cggccgacga gaggtcgacc accacgtcct
     2881 gtccgttggc gatcgcggtg cgcgcgtcgt gcttgaggtc ggcatacaag tgctgcaggc
     2941 gctcggcggg cggctcgtcc tggtgccagc cgtcgtccca gacagcctcg tccattgaga
     3001 gccgcaccgc cccacctgca gccagttgac gggcgtgggt ggtcttgccc gcgccggcgg
     3061 gcccgcacaa caggatcaca agagccatgc gccgagagta gttgtcggtg cgggctgctt
     3121 gatcggccgg cgtccagagc gcgtgcgcga ccccgtcgat cccgaaagag gagccatggc
     3181 attctcactc cccacgcaac cggggaggcg gcggtgctgt gcagcttcgt tcccgcacag
     3241 ttcgcccagg tgcgcagcag tgggctcggc ctcagcgacg agcagttgcc gcctacgagt
     3301 tcaacgcgct cggtgacggc gaagaatggc acgactggag ttgactctca cccgccctgc
     3361 aaacagtgtg tgagttcgga ccttgagtac aaggcccaga cccacactgt cttttcggtc
     3421 gcttttcagg tcgtcccccc ggccgctagt cctgcggcaa ggaaatgtcc tccggcgtct
     3481 tgtcggggcg ccagccccgc cagaccggca tgcgcagccg gcccgcgttc gtccagacgc
     3541 cgtagccgac ctcgccgacc aggatcggct ccacccagtg ggcccccttg gcatccgccc
     3601 ggggcacgtc gctcaccggc gccgccggca cctcgatctc gcccaggacc tgccgggtgt
     3661 cgaccagatc cttctcggtg aagcccgagc cgacccgacc gacataggtg agaccggcat
     3721 cgttgggcac cgccatcagc agtgcgccga tggtgcgggc ccgggcactc tcgcccggcg
     3781 tccacccgat gatcaccacc tcctgggtga gcacatgctt gatcttgacc caggtgtgcg
     3841 cgcggcgtcc gggctggtag acgctgtcgg ggcgcttggc cacgacaccc tccagcccca
     3901 gctcggcgct gatcgccagc gcctcgtcgg catcggtgcc cagctgcgta cccaattgga
     3961 cccgccgggt gccctccccc agcaactcgg ccagcttctg ccgccgtgcc gtgtacggct
     4021 cgcggatcag cgagcggccg tcgagataca gcaggtcgaa caccatgtag taggcctcgc
     4081 cggggtactg gctgcgattc tgcaatcgct cgaagctgga gcggccctgc gcatcgagca
     4141 gcacggcctc gccgtcgagc accgcctgct ggccgtccag cagccggccc agctcggcaa
     4201 gctcgggaaa gtcgcgggtc aggtcgaggc cgcggcggct ggtcaacgtc accgtgcccc
     4261 ccaccacggc ggcgatggcg cggtagccgt cccacttcac ctcgaacacc cagtcgccat
     4321 cgcgtacgtc cgcgggcgtg ccggcggtgg ccagcatcgg agcgaatgag ggcacctcga
     4381 actcgggcct ggtctgctca tcagccccgt cccgtggcgc catcaggtgg atcagccagt
     4441 tcttcgggtc gccgcccagc ttggtgtgca gcagagcgaa ccggcgcggc accccaccca
     4501 ggccgccgcc gggccgtccg gtgagggtgg cgatcacctc tttgccgtcg cgccacttct
     4561 ccaactcgaa ggtgccggcg tcccagatgc gcacgctgcc gccgccgtac tcgccgcgcg
     4621 g
//
LOCUS       DAOSUG010000220         4611 bp    DNA     linear   ENV 10-JUN-2024
DEFINITION  MAG TPA_asm: Micropruina sp. isolate AMR_MDS_2195
            NODE_8056_length_4611_cov_4.144644, whole genome shotgun sequence.
ACCESSION   DAOSUG010000220 DAOSUG010000000
VERSION     DAOSUG010000220.1
DBLINK      BioProject: PRJNA925477
            BioSample: SAMN32864262
            Sequence Read Archive: SRR4236673
KEYWORDS    WGS; ENV; Metagenome Assembled Genome; MAG; Third Party Data; TPA;
            TPA:assembly.
SOURCE      Micropruina sp. (wastewater metagenome)
  ORGANISM  Micropruina sp.
            Bacteria; Bacillati; Actinomycetota; Actinomycetes;
            Propionibacteriales; Nocardioidaceae; Micropruina.
REFERENCE   1  (bases 1 to 4611)
  AUTHORS   Abdulkadir,N., Saraiva,J.P., Zhang,J., Stolte,S., Gillor,O.,
            Harms,H. and Rocha,U.
  TITLE     Genome-centric analyses of 165 metagenomes show that mobile genetic
            elements are crucial for the transmission of antimicrobial
            resistance genes to pathogens in activated sludge and wastewater
  JOURNAL   Microbiol Spectr 12 (3), e0291823 (2024)
   PUBMED   38289113
REFERENCE   2  (bases 1 to 4611)
  AUTHORS   Abdulkadir,N.
  TITLE     Direct Submission
  JOURNAL   Submitted (24-MAR-2023) Environmental Microbiology, Helmholtz
            Center for Environmental Research -UFZ, Permoserstr 15, Leipzig
            04318, Germany
COMMENT     The annotation was added by the NCBI Prokaryotic Genome Annotation
            Pipeline (PGAP). Information about PGAP can be found here:
            https://www.ncbi.nlm.nih.gov/genome/annotation_prok/
            
            ##Genome-Assembly-Data-START##
            Assembly Method        :: SPAdes v. 3.11.1
            Genome Representation  :: Full
            Expected Final Version :: Yes
            Genome Coverage        :: Not Applicable
            Sequencing Technology  :: Illumina MiSeq
            ##Genome-Assembly-Data-END##
            
            ##Genome-Annotation-Data-START##
            Annotation Provider               :: NCBI
            Annotation Date                   :: 05/04/2023 04:57:10
            Annotation Pipeline               :: NCBI Prokaryotic Genome
                                                 Annotation Pipeline (PGAP)
            Annotation Method                 :: Best-placed reference protein
                                                 set; GeneMarkS-2+
            Annotation Software revision      :: 6.5
            Features Annotated                :: Gene; CDS; rRNA; tRNA; ncRNA
            Genes (total)                     :: 2,776
            CDSs (total)                      :: 2,750
            Genes (coding)                    :: 2,714
            CDSs (with protein)               :: 2,714
            Genes (RNA)                       :: 26
            tRNAs                             :: 24
            ncRNAs                            :: 2
            Pseudo Genes (total)              :: 36
            CDSs (without protein)            :: 36
            Pseudo Genes (ambiguous residues) :: 0 of 36
            Pseudo Genes (frameshifted)       :: 2 of 36
            Pseudo Genes (incomplete)         :: 32 of 36
            Pseudo Genes (internal stop)      :: 2 of 36
            ##Genome-Annotation-Data-END##
FEATURES             Location/Qualifiers
     source          1..4611
                     /organism="Micropruina sp."
                     /mol_type="genomic DNA"
                     /submitter_seqid="NODE_8056_length_4611_cov_4.144644"
                     /isolate="AMR_MDS_2195"
                     /isolation_source="Activated sludge of municipal
                     wastewater treatment plants"
                     /db_xref="taxon:2737536"
                     /environmental_sample
                     /geo_loc_name="Canada"
                     /lat_lon="49.25 N 123.1 W"
                     /collection_date="2015-06-16"
                     /metagenome_source="wastewater metagenome"
                     /note="metagenomic"
     gene            <1..1272
                     /locus_tag="PLF56_08120"
     CDS             <1..1272
                     /locus_tag="PLF56_08120"
                     /inference="COORDINATES: protein motif:HMM:NF019368.2"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_function: GO:0004559 - alpha-mannosidase activity
                     [Evidence IEA];
                     GO_process: GO:0006013 - mannose metabolic process
                     [Evidence IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="glycoside hydrolase family 38 C-terminal
                     domain-containing protein"
                     /protein_id="HOQ53566.1"
                     /translation="EPAETGEPLGEPAEASGGEPPEVDEPLVDPAEASGGDRPAPTLL
                     VNASPVAQQGVAAGAVAAGAPAARSATAVDDGFVLASEALSVRIDGSGHVVGAVDLTS
                     GRDAIAEGERGNLLQLHQDFPNLWDAWDVDAAYRQMRVDLDAADEVLLDSEVVVVRRS
                     FGASSVEQRLYLAPDGRSLLIDNTVDWHEREKFLKLAFPIDVFTDTAVAETQFGFHAR
                     TTHDNTSWENARFETHAQRWFLVQEPGFGVAFANDSSYGFDVTRRRRGSTLVQDVRFS
                     MLRAPRFPDPRTDLGVQRMRFAIAVGADEARSTELGWALNVPARAAGGRALEALVSSS
                     DPRVLISAVKLADDRSGDLVVRVYESTGGRAASEIRVALPVSAVSAADLLERPGEPLG
                     HCPTDDGATIPLTLRPFQVQTLRITCGERST"
     gene            1847..2677
                     /locus_tag="PLF56_08125"
     CDS             1847..2677
                     /locus_tag="PLF56_08125"
                     /inference="COORDINATES: protein motif:HMM:NF024107.2"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="alpha/beta hydrolase"
                     /protein_id="HOQ53567.1"
                     /translation="MSRARLAVAGALVVLLQVVAAFGTAGPARADSGLSVSSRTVTVS
                     SQGRSFSALLVQPTGSRPGAYPVVTFGHGFAQSASRYGSTLNALAARGYIVIAPNSQT
                     GLLPSHSAFAKDLGAAIAWGHATQPNSHPTLDAVLGHSMGGGAAVLAASANPSIDTVA
                     TLAAANTNPSAATAARSLSVPALFVVGSSDSVVKPSNTRAIYDAAPSPATWVSITGGY
                     HCGFLDSSSFFGLGCDNGSISRSQQLALSNTLLGDWLDATLKSGPAFNRPVGTTGEQK
                     "
     gene            2770..2845
                     /locus_tag="PLF56_08130"
     tRNA            2770..2845
                     /locus_tag="PLF56_08130"
                     /product="tRNA-Lys"
                     /inference="COORDINATES: profile:tRNAscan-SE:2.0.12"
                     /note="Derived by automated computational analysis using
                     gene prediction method: tRNAscan-SE."
                     /anticodon=(pos:2803..2805,aa:Lys,seq:ttt)
     gene            complement(2971..3288)
                     /locus_tag="PLF56_08135"
     CDS             complement(2971..3288)
                     /locus_tag="PLF56_08135"
                     /EC_number="3.1.-.-"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_014846112.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_function: GO:0003677 - DNA binding [Evidence IEA];
                     GO_function: GO:0003723 - RNA binding [Evidence IEA];
                     GO_function: GO:0004521 - endoribonuclease activity
                     [Evidence IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="type II toxin-antitoxin system PemK/MazF family
                     toxin"
                     /protein_id="HOQ53568.1"
                     /translation="MRGDIYRLRTSRTAEGHEQRGPRYGVVVQSDDLLLSTILVAPTS
                     TSALPTDFRPRITMDGTTTYVLVEQTAAVNAETRFGEFAGRLGPSELADLDRALTLVL
                     GLY"
     gene            complement(3278..3511)
                     /locus_tag="PLF56_08140"
     CDS             complement(3278..3511)
                     /locus_tag="PLF56_08140"
                     /inference="COORDINATES: protein motif:HMM:NF013561.2"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_process: GO:0006355 - regulation of DNA-templated
                     transcription [Evidence IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="ribbon-helix-helix protein, CopG family"
                     /protein_id="HOQ53569.1"
                     /translation="MSQIVIRVDEETTAALAHLVELTGQNRSEVVRDAIRAAEREAVL
                     ARVRQQAKQVREDPADRAEMLGLADEMEQLRAW"
     gene            4063..>4611
                     /locus_tag="PLF56_08145"
     CDS             4063..>4611
                     /locus_tag="PLF56_08145"
                     /inference="COORDINATES: protein motif:HMM:NF024734.2"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="type IV toxin-antitoxin system AbiEi family
                     antitoxin domain-containing protein"
                     /protein_id="HOQ53570.1"
                     /translation="MERREAVDVVASLAEGQWGMFTTAQAEELGVSRVDVARLVSGQL
                     ARRLRHGVHIMAGAPSSPIEEIRAEWLATEPSRSAGARRDDPEPVIVSDESAAAVHGV
                     GDLSPGGVHLTSARRLQSRQRWVKLHRRQISEREYSWIDGLPVTTPRRTLEDLANSGR
                     WEPDQLRALANDAIGRGLIPPAD"
BASE COUNT          728 a         1556 c         1545 g          782 t
ORIGIN      
        1 gaacccgctg aaaccggcga accgttgggc gagcctgccg aggcctcagg gggcgagcct
       61 cccgaggttg acgaaccgtt ggtcgatcct gccgaggcct caggtggcga tcgccctgca
      121 ccgacgctgc tggtcaatgc gtcgcccgtg gcgcaacagg gcgtcgccgc aggagccgtg
      181 gcggcaggcg ctccggctgc ccgctcggcg acggcggtcg acgacgggtt cgtgctggcc
      241 agcgaggcgc tgagtgtgag aatcgacggg tccggacacg tggtcggcgc ggtcgacctg
      301 acttcgggac gcgacgccat cgcggaaggc gagcgcggca acctcttgca actacatcag
      361 gatttcccca acctctggga cgcctgggac gtcgatgccg cctaccgaca gatgcgggtc
      421 gacctggacg ccgccgacga ggtgctgctc gacagcgagg tggtcgtggt gcgccgctcg
      481 ttcggggcgt cgtccgtcga gcagcggctg tacctggcgc cggacggacg ctcgctactg
      541 atcgacaaca ccgtcgactg gcacgagcgg gagaagttcc tcaagctggc ctttccgatc
      601 gacgtgttca ccgacaccgc cgtggccgag acccagttcg gcttccacgc ccgcaccacc
      661 cacgacaaca ccagttggga gaacgcccgc ttcgagaccc acgcccagcg ctggttcctg
      721 gtgcaggagc cggggttcgg cgtggcgttc gccaacgact cgtcctacgg cttcgacgtg
      781 acccgtaggc gccgcgggag caccctggtg caggacgtcc ggttctcgat gttgcgggcc
      841 ccccggtttc ccgacccgcg gaccgacctc ggggtgcagc gcatgcgctt cgcgatcgcc
      901 gtcggagccg atgaagcgcg ctcaaccgaa ctgggctggg cgctcaacgt gccggcacgc
      961 gcggctggcg gacgggcact ggaggcattg gtcagctcga gcgatccgcg ggtgctgatc
     1021 agcgcggtca aactggcgga tgatcgctcg ggcgacctgg tcgtccgggt gtacgagtcg
     1081 acggggggcc gggcggcgtc cgagatccgg gtcgcacttc ccgtgtccgc agtaagcgcc
     1141 gcggacctgc tcgaacgccc cggcgagccc cttggccact gccccaccga cgacggcgcg
     1201 acgattccgc tgactcttcg accgttccag gtgcagaccc tgagaatcac ctgcggcgag
     1261 cgttccacgt gatccagaga tcgctagccg ctgatcaccc ctggggtcgc gctcgtcgag
     1321 ccccctcgtg ggcagtggtt tcgacacgct caacaacgga tggcgccttg atcagcgctc
     1381 cccactgggg cgctgcgaaa gggtcgaaac tgaaatccct gcaccagtga gccgggtcca
     1441 cgctcgttca ggcgggagtc tgctcactgg tgctgggatt tcaggcgccg cggtctgcag
     1501 agcgctgacc tggcgcttgt cgagttcgtc gagacgcctc ggtggacaga ggttccgtca
     1561 accgcagcca acgatcgtgc gttgatcagc actcccccac gagggcgctg cgagagggcc
     1621 cgaactgaaa tgcctgcacc agtgaaccgg gtcgccgctc gttcaggcgg gactctggcc
     1681 actggcgctg ggatttcagg cgccgcggtc tgcgcatcca gtcccgtcag gtcggcttgc
     1741 ctgtgaacga gctgtcgaga tccatcacga ctgccccgtg aagctactgg gctcggtcag
     1801 ccctccggcc ctttcagccg cccgactccg cgcttatgct ctcgccatgt ctcgcgctcg
     1861 tctcgccgtc gccggcgcgc tggtcgtgct tcttcaggtt gtcgcggcat tcggcactgc
     1921 cggccccgcc cgcgccgatt cgggcctcag cgtcagcagt cgcacggtga ccgtcagtag
     1981 ccagggacgc agcttcagcg ccctgctcgt gcagccgacg ggctcacgcc ccggcgccta
     2041 cccggtggtg acgttcggac acggcttcgc gcagtcggcc agccggtacg gctccacgct
     2101 caacgccttg gccgctcgcg gctacatcgt gatcgcgccg aacagccaga ccggtctgct
     2161 gcccagccac tcggccttcg cgaaagacct gggcgcggcc atcgcctggg ggcacgccac
     2221 ccagcccaac tcgcatccca ccctggacgc cgttctgggc cactcgatgg gcggcggcgc
     2281 ggccgtactc gccgcgtctg cgaaccccag catcgacacc gtcgcaacgc tggctgcggc
     2341 caacaccaac ccgtcagcgg cgaccgccgc ccgctcgctt tcggtgcctg cgctgttcgt
     2401 ggtgggttca tccgattcgg tggtgaagcc gtccaacacg cgggcgatct acgatgccgc
     2461 tccgtccccg gccacctggg tgtccatcac cggcggctac cactgcgggt tcctggactc
     2521 gtcatcgttc ttcggactgg gttgcgacaa cggatccatc tcacgctcgc aacagctcgc
     2581 actgtcgaac accctgctcg gcgattggct ggacgccacc ctgaagtcgg ggccggcctt
     2641 caaccgtccg gtcggcacca cgggcgagca gaagtagcgc ccttccggca cgatcgcgag
     2701 cctgggacag cgacccggct gaggattgcg gtcgcccaca ggcggtagag tgagcaagcg
     2761 cgttcgcgcg ggcctatagc tcaattggca gagcagcgga cttttaatcc gcgggttctg
     2821 ggttcgattc ccagtgggcc caccagggat tgcatcggtg cgaccagctg acggccggca
     2881 ccggctacga ctacgtcacc cgaaacacag cgacgatgtg gccacggccg ggattctccc
     2941 gtcaagaggg cgatcaccgt caggcggtgg tcagtacaga ccgaggacca gggtgagggc
     3001 tcggtcgaga tcagccagtt cggacgggcc gagccggccg gcgaactcgc cgaaccgggt
     3061 ctctgcgttc acggccgccg tctgctcgac cagcacgtac gtcgttgtgc cgtccatggt
     3121 gatccggggc cggaaatcgg tcgggagtgc gctggtcgag gtcggtgcga ccaggatcgt
     3181 ggagagcagc aggtcgtcgg actggacgac cacgccgtag cggggccccc gctgttcgtg
     3241 gccttcggcg gttcgactgg tgcgcagccg gtagatgtca ccacgcacgc agctgctcca
     3301 tctcgtcggc cagtcccagc atttcggcgc gatcggcggg atcctcacgc acctgcttcg
     3361 cctgttgccg cacccgcgcc aggacggctt cccgctcggc agcgcggatc gcatcccgta
     3421 ctacctcgga gcgattctgg ccggtcagct cgacgaggtg cgccaaggcc gccgttgtct
     3481 cctcgtctac cctgatcacg atctgactca tgaatgtata ctactgcctg cgatgcgggg
     3541 cgcaatgcaa acttcctcga cgcaggcgct cgcgatcgcc ttctggctga cagcgagcac
     3601 ctcggcccgt ggctcattct cggccgcgcc ctgcggcagg gtcaccgcgt cgtgcagctg
     3661 gatgccgtac ccattcgagg ctcgccaacg gtgacggtct gtgcgcttgc cgcgccgtcc
     3721 ggcagaacga tctgctcgag cggctgtccg cacgagcgga aggatctggt cagcgagggt
     3781 tacgtacaca tcttgccggt tgctccgtgt gtgattgcag ccaagtggtc ggccatcggt
     3841 gaaacggagg atgacctccg ctattcgaga acggacgtgt tcgagccgac tgggtcgacg
     3901 tcactgtgga catccgggca gtcgacgagg cagcagccgc agacgcgggt gccgtcggag
     3961 aggaccaggt cggcgtcgtt ggtgaaggaa cagccgtcct tgtgacacgt gaacgtgcgt
     4021 gatcgatgct gcaatagtta ccaggactgt aacatttgca gtatggaaag gcgagaggcg
     4081 gtcgacgtgg ttgcttcatt ggcggagggc cagtggggca tgttcacaac cgcgcaagca
     4141 gaggagctgg gcgtctcccg cgtggacgtg gcgcgcttgg tgagcggtca gcttgcgcgg
     4201 cgattgcgtc acggtgtgca catcatggcc ggggcgccgt ccagtcccat tgaggagatt
     4261 cgggccgagt ggctcgccac cgaacccagc cggtcggcgg gggcacgaag ggacgaccct
     4321 gaaccagtga tcgtctccga cgaaagcgcg gcggccgtcc acggcgtcgg cgacttatcg
     4381 cctggaggcg tgcacctcac ctcggctcga cgactccaat ctcgacagcg ctgggtgaag
     4441 ctgcatcgcc gtcagatcag cgaacgtgaa tacagctgga tagacggact cccggtcacc
     4501 acgcctcggc gcactcttga agacctcgcg aactccggta gatgggaacc ggaccagttg
     4561 cgggccctcg cgaacgacgc gatcggtcgt ggcctgattc ctcccgcgga c
//
LOCUS       DAOSUG010000221         4605 bp    DNA     linear   ENV 10-JUN-2024
DEFINITION  MAG TPA_asm: Micropruina sp. isolate AMR_MDS_2195
            NODE_8062_length_4605_cov_3.401099, whole genome shotgun sequence.
ACCESSION   DAOSUG010000221 DAOSUG010000000
VERSION     DAOSUG010000221.1
DBLINK      BioProject: PRJNA925477
            BioSample: SAMN32864262
            Sequence Read Archive: SRR4236673
KEYWORDS    WGS; ENV; Metagenome Assembled Genome; MAG; Third Party Data; TPA;
            TPA:assembly.
SOURCE      Micropruina sp. (wastewater metagenome)
  ORGANISM  Micropruina sp.
            Bacteria; Bacillati; Actinomycetota; Actinomycetes;
            Propionibacteriales; Nocardioidaceae; Micropruina.
REFERENCE   1  (bases 1 to 4605)
  AUTHORS   Abdulkadir,N., Saraiva,J.P., Zhang,J., Stolte,S., Gillor,O.,
            Harms,H. and Rocha,U.
  TITLE     Genome-centric analyses of 165 metagenomes show that mobile genetic
            elements are crucial for the transmission of antimicrobial
            resistance genes to pathogens in activated sludge and wastewater
  JOURNAL   Microbiol Spectr 12 (3), e0291823 (2024)
   PUBMED   38289113
REFERENCE   2  (bases 1 to 4605)
  AUTHORS   Abdulkadir,N.
  TITLE     Direct Submission
  JOURNAL   Submitted (24-MAR-2023) Environmental Microbiology, Helmholtz
            Center for Environmental Research -UFZ, Permoserstr 15, Leipzig
            04318, Germany
COMMENT     The annotation was added by the NCBI Prokaryotic Genome Annotation
            Pipeline (PGAP). Information about PGAP can be found here:
            https://www.ncbi.nlm.nih.gov/genome/annotation_prok/
            
            ##Genome-Assembly-Data-START##
            Assembly Method        :: SPAdes v. 3.11.1
            Genome Representation  :: Full
            Expected Final Version :: Yes
            Genome Coverage        :: Not Applicable
            Sequencing Technology  :: Illumina MiSeq
            ##Genome-Assembly-Data-END##
            
            ##Genome-Annotation-Data-START##
            Annotation Provider               :: NCBI
            Annotation Date                   :: 05/04/2023 04:57:10
            Annotation Pipeline               :: NCBI Prokaryotic Genome
                                                 Annotation Pipeline (PGAP)
            Annotation Method                 :: Best-placed reference protein
                                                 set; GeneMarkS-2+
            Annotation Software revision      :: 6.5
            Features Annotated                :: Gene; CDS; rRNA; tRNA; ncRNA
            Genes (total)                     :: 2,776
            CDSs (total)                      :: 2,750
            Genes (coding)                    :: 2,714
            CDSs (with protein)               :: 2,714
            Genes (RNA)                       :: 26
            tRNAs                             :: 24
            ncRNAs                            :: 2
            Pseudo Genes (total)              :: 36
            CDSs (without protein)            :: 36
            Pseudo Genes (ambiguous residues) :: 0 of 36
            Pseudo Genes (frameshifted)       :: 2 of 36
            Pseudo Genes (incomplete)         :: 32 of 36
            Pseudo Genes (internal stop)      :: 2 of 36
            ##Genome-Annotation-Data-END##
FEATURES             Location/Qualifiers
     source          1..4605
                     /organism="Micropruina sp."
                     /mol_type="genomic DNA"
                     /submitter_seqid="NODE_8062_length_4605_cov_3.401099"
                     /isolate="AMR_MDS_2195"
                     /isolation_source="Activated sludge of municipal
                     wastewater treatment plants"
                     /db_xref="taxon:2737536"
                     /environmental_sample
                     /geo_loc_name="Canada"
                     /lat_lon="49.25 N 123.1 W"
                     /collection_date="2015-06-16"
                     /metagenome_source="wastewater metagenome"
                     /note="metagenomic"
     gene            <1..320
                     /locus_tag="PLF56_08150"
     CDS             <1..320
                     /locus_tag="PLF56_08150"
                     /EC_number="1.4.4.2"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_016669947.1"
                     /note="acts in conjunction with GvcH to form
                     H-protein-S-aminomethyldihydrolipoyllysine from glycine;
                     Derived by automated computational analysis using gene
                     prediction method: Protein Homology."
                     /codon_start=3
                     /transl_table=11
                     /product="glycine dehydrogenase
                     (aminomethyl-transferring)"
                     /protein_id="HOQ53571.1"
                     /translation="SKAELDRFCDAMIAIRGEADEVAAGRWPADDNPLHNAPHTQFRV
                     AADEWTHPYPRTLAAYPGGVRLGSIGTGATDKYWPASARIDGVYGDRNLVCSCPPVES
                     YAD"
     gene            750..1841
                     /locus_tag="PLF56_08155"
     CDS             750..1841
                     /locus_tag="PLF56_08155"
                     /inference="COORDINATES: protein motif:HMM:NF015587.2"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="YhjD/YihY/BrkB family envelope integrity
                     protein"
                     /protein_id="HOQ53572.1"
                     /translation="MPDWLAQWVEKLKAIPWVAHLLRMQERFTERLGNQFAAAVTYFS
                     LMSLVPILLFAFAMLGMVLTVFRPDLLDQVSQLIETQFQNNPLGTQIVAVMERALAGW
                     ATLGLVALAIFAWSGATWMANLKSAIRAQMRPDFSVQEKKGNIVVETLANLGTLLLLL
                     VSILIMFSLAATATSLSQVVLGWLQAPDTWVSRLVVYVAPIPVSIAVGFVLFAFLYKV
                     SYQQRVSRRVWLLGALSGSVGLAVLQYSATLIWGMFSGNAAASIFGPVIIMMLFFNLF
                     ATLILMIAAWMATAELQPVYRSPLAQDVGDAPAELGDPVPMVHEAVAQKAMKVGMGTG
                     YLLGAATGVGLGAIIAGVLGWFARLVRRP"
     gene            1874..2998
                     /locus_tag="PLF56_08160"
     CDS             1874..2998
                     /locus_tag="PLF56_08160"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_017933341.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_function: GO:0030170 - pyridoxal phosphate binding
                     [Evidence IEA];
                     GO_process: GO:0009058 - biosynthetic process [Evidence
                     IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="aminotransferase class I/II-fold pyridoxal
                     phosphate-dependent enzyme"
                     /protein_id="HOQ53573.1"
                     /translation="MEPFHVMEVLKAVAERRRTHDDVVLLCVGQPSTPAPAAVRAAAE
                     QALHADVLGYTEAAGTRAFREAVATHYRDRYDLAVPADEVIVTTGSSGGFLLTLLAAF
                     DPDDLVAITRPGYPAYRNDIVATGCRLLELACGPATRYQPTVSMLESLPERPAGLVIA
                     SPANPTGTIIDGAELAAISAWCAANGVLLISDEIYHGIGFGRTTASARQYGRESVVLG
                     SLSKYQSMTGWRVGWLLLPEALRRPVELLQGNLAICAPALSQAAGVAAFGAEARAELD
                     AHVRRYAANRDLLLARLPELGITNVAPPDGAFYAWCDIGHLTDDSQRWCAEALAATGV
                     ALTPGIDFDPVEGHRFMRLSFCGTGAELTEGINRLVDWLG"
     gene            complement(3067..3561)
                     /gene="ybaK"
                     /locus_tag="PLF56_08165"
     CDS             complement(3067..3561)
                     /gene="ybaK"
                     /locus_tag="PLF56_08165"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_012920268.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_function: GO:0002161 - aminoacyl-tRNA editing activity
                     [Evidence IEA];
                     GO_process: GO:0043039 - tRNA aminoacylation [Evidence
                     IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="Cys-tRNA(Pro) deacylase"
                     /protein_id="HOQ53574.1"
                     /translation="MSGKHSGGTPATEALSAAEVTFTLHPYTHHDDSHDFGAEAAREL
                     GVDELRIFKTLVVDIGVGRPPLAVGIVPVAGQLDLRAFAAALDAKKAVMAKPEEAART
                     SGYVVGGISPIGQKTPLPTVLDETAQLFDTIFVSAGKRGLQVELSPTDLLAITGARWG
                     DIAR"
     gene            3720..>4605
                     /locus_tag="PLF56_08170"
     CDS             3720..>4605
                     /locus_tag="PLF56_08170"
                     /inference="COORDINATES: protein motif:HMM:NF012503.2"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_function: GO:0016831 - carboxy-lyase activity [Evidence
                     IEA];
                     GO_function: GO:0030170 - pyridoxal phosphate binding
                     [Evidence IEA];
                     GO_process: GO:0019752 - carboxylic acid metabolic process
                     [Evidence IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="pyridoxal-dependent decarboxylase"
                     /protein_id="HOQ53575.1"
                     /translation="MIEWIIDYHDRLDSLPVQSRVEPGDIAALLPEHPGDPDGFDALV
                     ADLDKVVVPGLTNWQHPGFFAWFPANTSYPAILAELVSAGLGVQGMSWATSPAVTEIE
                     TRMMDWMIDLLGLPDTFRSTSEGGGGVIQGSASEATLTSILAARHRATQGAVNVDSDT
                     TKLVAYATSQAHSSIEKGLRIAGIGTANLRVVPHDENFAMRPDALAELIEADRANGLV
                     PFWVCTTLGTTNSLAIDPLRAVAEVARARDLWLHVDAAMAGIATLCPEFRWLNDGLEL
                     VDSYSTNPHKWMGVTFDCN"
BASE COUNT          675 a         1548 c         1625 g          757 t
ORIGIN      
        1 agtcgaaggc cgagctcgac cggttctgcg acgccatgat cgcgatccgg ggcgaggcgg
       61 acgaggtggc ggcgggtcgg tggcccgccg acgacaatcc tctgcacaac gccccccaca
      121 cccagttcag ggtggctgcc gacgagtgga cgcacccgta cccgcgcacc ctggcggcct
      181 acccgggcgg cgtccggctg ggcagcatcg gcaccggcgc caccgacaag tactggccgg
      241 ccagcgcccg catcgacggt gtgtacggcg accgcaacct ggtctgctcg tgcccgccgg
      301 tggagagtta cgccgactga acgggacccg ggtgtgagat cccggcgttc gccgggatga
      361 cggatggggt cgggatgacg caagggaccg ggtccgagat cccggcgccg ggatgagtga
      421 aagggccggg atgacgcaag ggaccggggt gtgagatccc ggcgttcgcc gggatgacgg
      481 atggggtcgg gatgacgcaa gggaccgggt ccgagatccc ggcgttcgcc gggatgacgg
      541 aaggggccgg gatgacggaa ggggccggga tgacggatgg ggccgggacg acggagcgga
      601 ccggggtgtg agatcccggc gttcgccggg atgacgaaac tggcggggat gacggatggg
      661 gccggaggtc gacccgcccg caatggtgta cggcgctccg atgtcgtccg gctaggctga
      721 acgcgcccga tgaccgaagg gacgaaccga tgcctgactg gctcgcgcag tgggtggaga
      781 agctcaaggc gataccgtgg gtggcgcacc tgctgcgcat gcaggagcgg ttcaccgaac
      841 gcttgggcaa ccagttcgcc gcggccgtca cctacttctc gttgatgtcc ctggtgccga
      901 ttctgctgtt cgcgttcgcg atgctcggca tggtgctgac ggtgtttcgg cccgatctgc
      961 tcgatcaggt cagccagttg atcgagaccc agtttcagaa caacccgttg ggtacccaga
     1021 tcgtcgccgt gatggaacga gccctggcgg gctgggccac cctgggcctg gtcgccctgg
     1081 cgatcttcgc ctggtccggg gcaacctgga tggcgaacct gaagagcgcg atccgggccc
     1141 agatgcggcc ggacttcagc gtgcaggaga agaagggcaa catcgtcgtc gagacgctgg
     1201 ccaatctcgg cacgctgctt ctgctgctgg tcagcatcct gatcatgttc tcgctggcgg
     1261 ccaccgccac ctcgctgtcg caggtggtgc tcggctggct ccaggctccc gacacctggg
     1321 tgagccggct ggtggtgtac gtggcaccca ttccggtatc gatcgcggtc ggattcgtgc
     1381 tgttcgcctt cctgtacaag gtcagctacc agcagcgcgt ctcgcgccgg gtctggctgc
     1441 tgggcgcgct gtcgggctcg gtcgggctgg ccgtgctgca gtactccgcc accctcatct
     1501 ggggcatgtt ctcgggcaac gccgcggcat ccatcttcgg gccggtcatc atcatgatgc
     1561 tgttcttcaa cctgttcgcc accctgatcc tgatgatcgc cgcctggatg gccaccgctg
     1621 agttgcagcc ggtctaccgc tcaccgctgg cccaggacgt cggcgacgcg cctgccgagt
     1681 tgggcgaccc cgtgcccatg gtgcacgagg ccgtggcgca gaaggcgatg aaggtgggca
     1741 tgggtacggg ctatctgctc ggcgcggcga ccggcgtcgg gctgggcgcg atcatcgccg
     1801 gcgtgctggg ttggttcgcg cggctcgtcc gtcgaccgtg acgccgcggg gcgcgtctcg
     1861 acgcggcttc ggtgtggagc cctttcacgt gatggaggtg ctcaaggcgg tagcggagcg
     1921 tcgccgcacc cacgatgacg tggtgctgct ctgcgtcggg cagccgtcca caccggcgcc
     1981 ggcggcggtg cgtgcggcgg ccgagcaggc cctgcatgcc gacgtgctcg gctacaccga
     2041 ggccgccggt acccgcgcct ttcgcgaagc ggtcgccacg cactaccgcg accgctacga
     2101 cctcgccgtc ccagcggacg aggtgatcgt caccacggga tcctccggcg ggtttctgct
     2161 gacgctgctg gcggccttcg acccggacga tctggtggcg atcacccgtc cgggctaccc
     2221 cgcgtaccgc aacgacatcg tcgccaccgg gtgtcgcctg cttgagctcg cgtgtggccc
     2281 cgcaacccgt tatcagccca ctgtgtcgat gctggagtcg ctgcccgagc ggcccgccgg
     2341 cctggtgatc gccagcccgg ccaaccccac cggcaccatc atcgacggtg ccgaactcgc
     2401 ggccatcagc gcctggtgtg ccgccaatgg cgtgctgttg atcagcgacg agatctacca
     2461 cggcatcggc ttcggtcgca ccaccgcctc cgcccggcag tacgggcgcg agtcggtggt
     2521 gctgggctcg ttgagcaagt accagtcgat gaccggctgg cgggtcggtt ggttgctgct
     2581 gccggaggcg ctgcggcgtc cggtcgaact gctgcagggc aatctcgcga tctgcgcccc
     2641 ggcactgtcg caggcggccg gtgtcgcggc tttcggggcc gaggcccgag ccgagctgga
     2701 cgcccacgtg cgccgctacg ccgccaaccg cgacctgctg ctggcgcggc tgccggagct
     2761 gggcatcacc aacgtcgcgc cacccgatgg cgccttctac gcgtggtgcg acatcggcca
     2821 cctgaccgac gactcgcagc gctggtgcgc cgaggcgctc gccgccacgg gggtcgcgct
     2881 gaccccgggc atcgacttcg atccggtcga ggggcaccga ttcatgcggc tgtcgttctg
     2941 cggtaccggc gccgagctga ccgagggcat caatcgcctg gtggactggc tcggctgagc
     3001 ggatcgcctc gacccgtggg tcgcccgggc cccccactgg gggtcagcct cgctttcgga
     3061 agaacgtcac cgcgcgatgt cgccccaccg tgcgccagtg atcgcgagca ggtcggtggg
     3121 ggagagttcc acctgtagcc cgcgcttgcc ggcggagacg aagatcgtgt cgaacagctg
     3181 cgcggtctcg tccagcaccg tgggcagtgg agtcttctga ccgatcggag agataccgcc
     3241 gacgacgtag ccggaggtcc gcgccgcctc ctcgggcttg gccatcaccg ccttcttggc
     3301 atcgagcgcg gcggcgaagg ccctcaggtc gagttgccca gccaccggca cgatgccgac
     3361 ggcgagcggg ggacgtccga caccgatgtc gaccaccagc gtcttgaaga tgcgcagttc
     3421 gtccaccccc aactcgcggg cggcctcggc gccgaagtcg tgactgtcgt cgtggtgggt
     3481 gtagggatgc agggtgaatg tgacctcggc ggcgctcaac gcctcggtcg caggcgtgcc
     3541 cccggaatgc ttgccgctca ccggttcaac ctagtcgtgg ttcgtcgcca ccggccgcga
     3601 tggacggcgc ggcgcggccc tggcacccgt ccggtgcccg tcactacggc ggccaccggt
     3661 cgatccggga ttagggtgag gtcgtggatg cagcggagtt cagagagcac ggtcaccggt
     3721 tgatcgaatg gatcatcgac taccacgatc ggctcgactc gttgccggtg cagtcgcggg
     3781 tcgagccggg cgacatcgca gcgctgttgc ccgagcatcc cggcgatccg gacggcttcg
     3841 acgccctggt cgccgacctc gacaaggtgg tggttcccgg cctgaccaac tggcagcatc
     3901 cgggcttttt cgcctggttt ccggccaaca ccagctatcc ggcgattctg gccgaactcg
     3961 tctcggcggg gctgggcgtg cagggcatga gctgggccac cagcccagcc gtcaccgaga
     4021 tcgagacccg catgatggac tggatgatcg acctgctggg tctgcccgac acgttccgca
     4081 gcacgagcga gggcggcggc ggggtgatcc agggctccgc gagcgaggcc accctgacct
     4141 cgattctcgc ggcccgccac cgcgccaccc agggcgccgt caacgtcgac tccgacacga
     4201 ccaaactcgt cgcctacgcc acttcgcaag cgcattcgtc catcgagaag gggttgcgca
     4261 tcgccggcat cggcactgcg aacctgcggg tggtgccaca cgacgagaac ttcgccatgc
     4321 gccccgacgc gctcgcggaa ctgatcgagg ccgaccgcgc caacggactg gtgccgttct
     4381 gggtgtgcac gaccctgggc accaccaact cgctggcgat cgaccccttg cgcgcggtgg
     4441 ccgaggttgc ccgggcacgc gacctgtggc tgcatgtcga tgccgcgatg gcaggcatcg
     4501 ccaccttgtg ccccgagttc cgctggctga acgacggctt ggagttggtc gacagctaca
     4561 gcaccaaccc gcacaagtgg atgggcgtca ccttcgactg caacc
//
LOCUS       DAOSUG010000222         4571 bp    DNA     linear   ENV 10-JUN-2024
DEFINITION  MAG TPA_asm: Micropruina sp. isolate AMR_MDS_2195
            NODE_8126_length_4571_cov_2.894154, whole genome shotgun sequence.
ACCESSION   DAOSUG010000222 DAOSUG010000000
VERSION     DAOSUG010000222.1
DBLINK      BioProject: PRJNA925477
            BioSample: SAMN32864262
            Sequence Read Archive: SRR4236673
KEYWORDS    WGS; ENV; Metagenome Assembled Genome; MAG; Third Party Data; TPA;
            TPA:assembly.
SOURCE      Micropruina sp. (wastewater metagenome)
  ORGANISM  Micropruina sp.
            Bacteria; Bacillati; Actinomycetota; Actinomycetes;
            Propionibacteriales; Nocardioidaceae; Micropruina.
REFERENCE   1  (bases 1 to 4571)
  AUTHORS   Abdulkadir,N., Saraiva,J.P., Zhang,J., Stolte,S., Gillor,O.,
            Harms,H. and Rocha,U.
  TITLE     Genome-centric analyses of 165 metagenomes show that mobile genetic
            elements are crucial for the transmission of antimicrobial
            resistance genes to pathogens in activated sludge and wastewater
  JOURNAL   Microbiol Spectr 12 (3), e0291823 (2024)
   PUBMED   38289113
REFERENCE   2  (bases 1 to 4571)
  AUTHORS   Abdulkadir,N.
  TITLE     Direct Submission
  JOURNAL   Submitted (24-MAR-2023) Environmental Microbiology, Helmholtz
            Center for Environmental Research -UFZ, Permoserstr 15, Leipzig
            04318, Germany
COMMENT     The annotation was added by the NCBI Prokaryotic Genome Annotation
            Pipeline (PGAP). Information about PGAP can be found here:
            https://www.ncbi.nlm.nih.gov/genome/annotation_prok/
            
            ##Genome-Assembly-Data-START##
            Assembly Method        :: SPAdes v. 3.11.1
            Genome Representation  :: Full
            Expected Final Version :: Yes
            Genome Coverage        :: Not Applicable
            Sequencing Technology  :: Illumina MiSeq
            ##Genome-Assembly-Data-END##
            
            ##Genome-Annotation-Data-START##
            Annotation Provider               :: NCBI
            Annotation Date                   :: 05/04/2023 04:57:10
            Annotation Pipeline               :: NCBI Prokaryotic Genome
                                                 Annotation Pipeline (PGAP)
            Annotation Method                 :: Best-placed reference protein
                                                 set; GeneMarkS-2+
            Annotation Software revision      :: 6.5
            Features Annotated                :: Gene; CDS; rRNA; tRNA; ncRNA
            Genes (total)                     :: 2,776
            CDSs (total)                      :: 2,750
            Genes (coding)                    :: 2,714
            CDSs (with protein)               :: 2,714
            Genes (RNA)                       :: 26
            tRNAs                             :: 24
            ncRNAs                            :: 2
            Pseudo Genes (total)              :: 36
            CDSs (without protein)            :: 36
            Pseudo Genes (ambiguous residues) :: 0 of 36
            Pseudo Genes (frameshifted)       :: 2 of 36
            Pseudo Genes (incomplete)         :: 32 of 36
            Pseudo Genes (internal stop)      :: 2 of 36
            ##Genome-Annotation-Data-END##
FEATURES             Location/Qualifiers
     source          1..4571
                     /organism="Micropruina sp."
                     /mol_type="genomic DNA"
                     /submitter_seqid="NODE_8126_length_4571_cov_2.894154"
                     /isolate="AMR_MDS_2195"
                     /isolation_source="Activated sludge of municipal
                     wastewater treatment plants"
                     /db_xref="taxon:2737536"
                     /environmental_sample
                     /geo_loc_name="Canada"
                     /lat_lon="49.25 N 123.1 W"
                     /collection_date="2015-06-16"
                     /metagenome_source="wastewater metagenome"
                     /note="metagenomic"
     gene            <1..1349
                     /locus_tag="PLF56_08175"
     CDS             <1..1349
                     /locus_tag="PLF56_08175"
                     /inference="COORDINATES: protein motif:HMM:NF013079.2"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=3
                     /transl_table=11
                     /product="leucyl aminopeptidase family protein"
                     /protein_id="HOQ53576.1"
                     /translation="ADAAALKAHGFEAAAGTAIRLWSGSGAATVAGGVGAEPTLASVR
                     DAAAAIARTADAEPSLAIELPSLAGLDDAAVAEAVVEGAVLARYSYDALKAEPTRVPI
                     TTLTLVIAEGVDEASARTGAERGRVLAAATALTRDLANTPHNHLTATMLADFAQRFGA
                     ERGLDVEVFGPDEARELRLGGLLAINAGSVEPLRMIKLTYRPDGEPSRPVGLVGKGIT
                     YDAGGISLKPSDGVHAMMKNDMTGAAAILAAMGTLRELGCTNEVTGWLMCTNNMPSGS
                     ATALGDVLTMRNGLSVEVIDSDAEGRLVMADALVLAAESEPDAIIDIATLTGSAGRAL
                     GPDMGAVLGNSQATVDAVIAAGGVADEPVWQLPLHRPYLRMLDSTTADICNCAAVGLP
                     DAILASLFLEKFVADVPWAHIDIAGTAQADAERSVRVPGGSGFGARLLAHLLTRDW"
     gene            1412..3214
                     /locus_tag="PLF56_08180"
     CDS             1412..3214
                     /locus_tag="PLF56_08180"
                     /inference="COORDINATES: protein motif:HMM:NF018448.2"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="threonine/serine exporter family protein"
                     /protein_id="HOQ53577.1"
                     /translation="MTQHRVRRAVAASATAVALLVAPVIGWSTVVAQAVPAETGTAQP
                     YATDEATAAPSEADPEPTGTDDPPATTAPPATTAPPTPAPPSAPTPTTPTTAAPTPTA
                     APTESVPTATTTPSPGAPATMPAVPPAYTQAHGSPASWVIAVLALAVGGVAGFVLRRR
                     TLRAVRAAPAPIPAHTTSLPPLGSLQAPRGIFVEDAADKLHALESTGQAMIDAGYSVA
                     AVRQALEEVAEASGYPDTEVVVFPTALFVSARGLGEVRTGAVTSGASRLNLAQVDALD
                     DVVGEARYGRLDAAALIARIAAVRTLPPPYSPGRRVLAYGAASAGIAVLLGSSLLGTG
                     LAAALGLLIGWALTRTENVPQQYQALVTVTCAFVVGVVVLLVVRQGLDPGVLPSLIAP
                     LVTFLPGALLTTGVVELSTGHMMAGAGRLAAGAMQLVLLAAGVTAAATLVGVPSLELV
                     GADQPLGPLAPWLAVAVFGIGIVIHSNGRPGAIGWVLLVLYVAYGAQVLADIAVGGVL
                     SAFIGALAMTPVAVLVAQQPSGPAAFVSFLPAFWLLVPGALGLVGVTNLLDGNSAGLG
                     ALLTTMSTMVAIALGILAGSALGDRLAPTRRALI"
     gene            3241..4386
                     /locus_tag="PLF56_08185"
     CDS             3241..4386
                     /locus_tag="PLF56_08185"
                     /inference="COORDINATES: protein motif:HMM:NF012318.2"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="trypsin-like peptidase domain-containing
                     protein"
                     /protein_id="HOQ53578.1"
                     /translation="MNHRTSGLTALVLGLAIALSGCTGQTPTPTAPTTSLAATPVTPP
                     TVKPADPLQVKDLAAFSGVAKLEAGSNCTGTLIDTGVPSGPAYLLTNGHCVGDIARSA
                     QATTTNLDWFGTATFLAADGNQSDALTVDVTQLAYSTMRDTDTGIVRLKPSLAELKAL
                     GIRAVPIVAAQPAAGTKVVNVGVPVQDMMPDQWILRRGECTLGAQHTVIEAAWLWFGV
                     WSNDCPGIIQGSSGSPLFTLGADGSPTAIAAMINTTTGGATAANGGACSINHPCEVNH
                     GTASMVENTSYAQSVARLGRCFDASGAFDLGDSCPLPTATVWAELGGGAFRGGSLPNS
                     SAQTRGEPGRGSGGQGADGVGAARRRHRVPTRGDLPGRTQQGPAEGR"
BASE COUNT          627 a         1673 c         1604 g          667 t
ORIGIN      
        1 ccgccgacgc cgcagcgctc aaggcgcacg gcttcgaggc cgccgccggc accgcgatcc
       61 ggctgtggtc gggcagcggc gcagccacgg tggcgggcgg cgtcggcgcg gagcccactc
      121 tggcctccgt gcgcgatgcg gccgctgcga tcgcgcggac ggcggatgcc gagccctccc
      181 tggcgatcga actgccgagc ctcgcggggc tggacgatgc cgcggtcgcc gaagcggtgg
      241 tcgagggcgc ggtcctggcc cgctacagct acgacgcact gaaggccgag cccactcggg
      301 tgccgatcac cacactcacc ctggtgatcg ccgagggagt ggacgaggcc tcagcccgga
      361 ccggcgccga acgtggccgt gtgctggccg ccgcgaccgc gctgacccgc gacctggcca
      421 acaccccgca caaccatctc accgccacca tgctcgccga cttcgcacag cggttcggtg
      481 ccgaacgtgg cctcgacgtc gaggtgttcg gcccggacga ggcgcgtgag ttgcggctgg
      541 gcggcctgct ggcgatcaat gccggctccg tcgaaccgct gcgcatgatc aagctgacct
      601 accggcccga cggcgagccg agccgccccg tcgggctggt cggcaagggc atcacctatg
      661 acgccggcgg catcagcctc aagccgtccg acggggtgca cgccatgatg aagaacgaca
      721 tgacgggtgc ggcggcgatc ctggccgcca tgggcaccct gcgcgaactc ggctgcacca
      781 acgaggtgac cggctggttg atgtgcacca acaacatgcc gtccggctcg gcgaccgcgc
      841 tcggcgatgt gctcaccatg cgcaacgggt tgagcgtcga ggtgatcgac tccgatgccg
      901 agggccggct ggtgatggcg gacgccctgg tgctggccgc agagagcgaa ccggacgcga
      961 tcatcgacat cgccaccctg accggttcgg ccgggcgcgc gctgggcccc gacatgggtg
     1021 ccgtgctggg caacagccag gccaccgtcg atgcggtgat cgccgccggc ggcgtggcgg
     1081 acgagccggt gtggcaactg ccgctgcatc ggccctacct gcgcatgctg gactcgacca
     1141 ccgccgacat ctgcaactgc gccgcggtcg gactgcccga tgcgattctg gcctcgctgt
     1201 tcttggagaa gttcgtcgcc gacgtaccgt gggcccacat cgacatcgcg ggcaccgcgc
     1261 aggccgatgc cgaacgctcc gtgcgggtgc ccggcggtag cgggttcgga gcccggctgt
     1321 tggcgcacct gctgacccgg gactggtgac ccccgcggaa cctggacgtg gcggtttcgc
     1381 cagaagtccc gccagggagg acgatgggcg catgacccag catcgtgtac ggcgtgccgt
     1441 cgcggcctcc gccactgcgg tggcgctgtt ggtcgcgccg gtgatcggct ggtccaccgt
     1501 ggtcgcgcag gcggtgccgg ccgagaccgg gacggcgcag ccgtacgcga cggacgaagc
     1561 gaccgcggcc ccgtcggaag ccgatccgga gccgaccggc accgacgacc cgcccgcgac
     1621 cacggctccc ccggcgacca ccgcgccgcc cacgcctgcg ccgccgtccg caccgacgcc
     1681 gacgaccccc accaccgccg cgccgacacc gacggctgcg ccgaccgagt cggtccccac
     1741 ggccaccacc acccccagtc cgggggcacc ggcgacgatg cccgccgttc caccggcgta
     1801 cacccaggcg cacggatcac ccgcctcgtg ggtgatcgcg gtccttgccc tggcggtggg
     1861 cggggtcgcc ggcttcgtgc tgcgccgccg caccctgcgt gcggtgcgcg ctgcccccgc
     1921 gccgatcccg gcgcacacca cgtcgttgcc cccgctcggt agccttcagg ccccgcgcgg
     1981 catcttcgtc gaggacgcgg ccgacaagct ccacgcgctg gaatccaccg ggcaggcgat
     2041 gatcgatgcg ggctattcgg tcgcggccgt ccggcaggcg ttggaagagg tcgccgaggc
     2101 cagcggatat cccgacaccg aggtggtggt ctttccgacc gccctgttcg tctcggcgcg
     2161 cggactgggc gaggtgcgca ccggtgccgt caccagcgga gccagccggc tcaacctggc
     2221 ccaggtggac gccctggacg acgtggtcgg cgaagcccgg tacggccggc tcgacgcggc
     2281 cgcactgatc gcccggatcg ctgccgtacg caccctgccg ccgccctact cgcccggacg
     2341 ccgggtgctc gcctacggcg cagcctcggc cggcatcgcg gtactgctcg gctcgtccct
     2401 gctcggcacc gggctggccg cggcgctcgg cctactgatc ggctgggcgc tgacccgcac
     2461 cgagaacgtg ccgcagcagt atcaggcgct ggtcacggtc acctgtgcgt tcgtggtcgg
     2521 ggtggtggtg ctgctggtgg tccggcaggg cttggacccc ggggtgctgc cctcactgat
     2581 cgcccccctg gtcacttttc tgcccggcgc cctgctcacc accggtgtgg tggagttgtc
     2641 gaccggccac atgatggccg gtgccgggcg cctcgccgcc ggcgccatgc aactcgtgct
     2701 gctggcggcg ggcgtgacgg ccgccgccac cctggtcggg gtgcccagcc tcgagttggt
     2761 cggcgcggac cagccgctcg gcccgctggc cccgtggctg gctgttgccg ttttcggcat
     2821 cggcatcgtg atccactcca acggccggcc cggggcgatc ggctgggtgc tgctggtgct
     2881 gtacgtcgcc tacggtgccc aggtgctggc cgacatcgcg gtgggcggtg tgctgtccgc
     2941 gttcatcggc gcactggcca tgacgccggt ggcggtactg gtggcccagc agccgtccgg
     3001 gccggcggcc ttcgtcagct tcttaccggc gttctggctg ctggtccccg gcgcgctcgg
     3061 actggtcggt gtcaccaatc tgttggacgg caactcggcc ggtttgggcg cactgctcac
     3121 caccatgtcg accatggtgg cgatcgctct gggcattctg gccggttcgg cactgggtga
     3181 ccggctcgcg ccgacccgcc gggctttgat ctaggcgacc acgccattga aggagcagct
     3241 atgaaccacc ggaccagcgg cctcacggcc ctggtgctag gcctcgccat cgcgctgagc
     3301 ggttgcaccg gccagacgcc gacgcccacc gcacccacca ccagcctcgc cgcgacgccc
     3361 gtcacgccac ccaccgtgaa gccggcggac ccattgcagg tcaaggatct ggcggccttc
     3421 agcggcgtcg ccaagctcga ggccgggtcg aactgcaccg gcaccctgat cgacaccggc
     3481 gtgccatccg gccccgcgta cctgctgacc aacggtcact gcgtcggcga tatcgctcgt
     3541 tcggcgcagg cgacgaccac gaacctggat tggttcggca ccgccacttt cctcgccgcg
     3601 gacggcaacc agtccgatgc gctcaccgtg gacgtcaccc agttggccta ctcgacgatg
     3661 cgtgacactg acaccggcat cgtccggctg aagccctcgc tggccgagct gaaggcgctg
     3721 ggcatccggg ccgttccgat cgtggccgcc caacccgccg ccggcacgaa ggtggtcaac
     3781 gtcggggtac cggtgcagga catgatgccc gaccagtgga tcctgcgccg cggcgaatgc
     3841 acgctgggcg cgcaacacac cgtgatcgaa gcggcgtggc tgtggttcgg ggtgtggagc
     3901 aacgactgcc ccggcatcat tcagggctcc agcggctcgc cgctgttcac cctaggtgcg
     3961 gacggatcgc ccaccgcgat cgccgccatg atcaacacca ccaccggcgg agcgaccgcg
     4021 gccaacggcg gggcctgttc gatcaatcac ccctgcgagg tcaatcacgg cacggcgtcc
     4081 atggtcgaga acaccagcta tgcccagagc gtggcgcggc tcggacgatg cttcgacgcg
     4141 tccggcgcat tcgatctcgg cgattcgtgc cccttgccca ccgcgaccgt ctgggcggag
     4201 ttgggcggag gcgcgttccg cggcggcagc ctgccgaact cgtcggcgca aacccgaggt
     4261 gagcctggtc gggggtcagg cgggcagggt gcagacggcg ttggtgccgc tcggcgacgg
     4321 caccgcgtgc cgacgcgcgg agacctacca gggcgcaccc agcaaggccc tgccgaaggc
     4381 cggtgaggaa tgggaacagg tcggcgtgac cgtagcggtc gtcctgccga aggtcgaggg
     4441 tcgtcacctg ctctgcgcgg tcaacgacgg cgacctggcc ggcgcggcat cggtgctttt
     4501 cgacgtggac atcacacccc cacgattccc ggcttcggcc gacgtcgaag acatcggcga
     4561 cggtgccgtg g
//
LOCUS       DAOSUG010000223         4547 bp    DNA     linear   ENV 10-JUN-2024
DEFINITION  MAG TPA_asm: Micropruina sp. isolate AMR_MDS_2195
            NODE_8175_length_4547_cov_4.054764, whole genome shotgun sequence.
ACCESSION   DAOSUG010000223 DAOSUG010000000
VERSION     DAOSUG010000223.1
DBLINK      BioProject: PRJNA925477
            BioSample: SAMN32864262
            Sequence Read Archive: SRR4236673
KEYWORDS    WGS; ENV; Metagenome Assembled Genome; MAG; Third Party Data; TPA;
            TPA:assembly.
SOURCE      Micropruina sp. (wastewater metagenome)
  ORGANISM  Micropruina sp.
            Bacteria; Bacillati; Actinomycetota; Actinomycetes;
            Propionibacteriales; Nocardioidaceae; Micropruina.
REFERENCE   1  (bases 1 to 4547)
  AUTHORS   Abdulkadir,N., Saraiva,J.P., Zhang,J., Stolte,S., Gillor,O.,
            Harms,H. and Rocha,U.
  TITLE     Genome-centric analyses of 165 metagenomes show that mobile genetic
            elements are crucial for the transmission of antimicrobial
            resistance genes to pathogens in activated sludge and wastewater
  JOURNAL   Microbiol Spectr 12 (3), e0291823 (2024)
   PUBMED   38289113
REFERENCE   2  (bases 1 to 4547)
  AUTHORS   Abdulkadir,N.
  TITLE     Direct Submission
  JOURNAL   Submitted (24-MAR-2023) Environmental Microbiology, Helmholtz
            Center for Environmental Research -UFZ, Permoserstr 15, Leipzig
            04318, Germany
COMMENT     The annotation was added by the NCBI Prokaryotic Genome Annotation
            Pipeline (PGAP). Information about PGAP can be found here:
            https://www.ncbi.nlm.nih.gov/genome/annotation_prok/
            
            ##Genome-Assembly-Data-START##
            Assembly Method        :: SPAdes v. 3.11.1
            Genome Representation  :: Full
            Expected Final Version :: Yes
            Genome Coverage        :: Not Applicable
            Sequencing Technology  :: Illumina MiSeq
            ##Genome-Assembly-Data-END##
            
            ##Genome-Annotation-Data-START##
            Annotation Provider               :: NCBI
            Annotation Date                   :: 05/04/2023 04:57:10
            Annotation Pipeline               :: NCBI Prokaryotic Genome
                                                 Annotation Pipeline (PGAP)
            Annotation Method                 :: Best-placed reference protein
                                                 set; GeneMarkS-2+
            Annotation Software revision      :: 6.5
            Features Annotated                :: Gene; CDS; rRNA; tRNA; ncRNA
            Genes (total)                     :: 2,776
            CDSs (total)                      :: 2,750
            Genes (coding)                    :: 2,714
            CDSs (with protein)               :: 2,714
            Genes (RNA)                       :: 26
            tRNAs                             :: 24
            ncRNAs                            :: 2
            Pseudo Genes (total)              :: 36
            CDSs (without protein)            :: 36
            Pseudo Genes (ambiguous residues) :: 0 of 36
            Pseudo Genes (frameshifted)       :: 2 of 36
            Pseudo Genes (incomplete)         :: 32 of 36
            Pseudo Genes (internal stop)      :: 2 of 36
            ##Genome-Annotation-Data-END##
FEATURES             Location/Qualifiers
     source          1..4547
                     /organism="Micropruina sp."
                     /mol_type="genomic DNA"
                     /submitter_seqid="NODE_8175_length_4547_cov_4.054764"
                     /isolate="AMR_MDS_2195"
                     /isolation_source="Activated sludge of municipal
                     wastewater treatment plants"
                     /db_xref="taxon:2737536"
                     /environmental_sample
                     /geo_loc_name="Canada"
                     /lat_lon="49.25 N 123.1 W"
                     /collection_date="2015-06-16"
                     /metagenome_source="wastewater metagenome"
                     /note="metagenomic"
     gene            <1..1608
                     /locus_tag="PLF56_08190"
     CDS             <1..1608
                     /locus_tag="PLF56_08190"
                     /EC_number="2.4.-.-"
                     /inference="COORDINATES: protein motif:HMM:NF013100.2"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_function: GO:0016757 - glycosyltransferase activity
                     [Evidence IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="transglycosylase domain-containing protein"
                     /protein_id="HOQ53579.1"
                     /translation="GYLNTVYFGRGAYGIQAAAQAYFGKPAAKLTDAQAIALTSIVNN
                     PGNLDPAKGDKQAADLLERYQYTINGMVEMGTMTEAQKAEIYYQLPKFPKVERDSQYG
                     GPKGFLLNMVQDELKAKGFSDEQIAGGGLTIRTTFDVKAQEAAVEAAQKVTAQTAAAG
                     NKKASNLHAGLASVDVATGGVLALYGGPDFVKNSRNWATTPRPTGSTFKPYALGAALA
                     QGFTLNDTLNGSQFVPPGAARPVRNAGDRNYGTVTLQQATTHSINTAFVDLETQMKDG
                     PESTLAFAQAAGLPKTAGWDPTVVVPLGVPEVSPLGQATGFATFARGGELIETHVVAE
                     VKNASGATLFKADTSAEQTIEADVANDVAYALTHVVQDGTASRAGGMGFPIAGKTGTY
                     YLRDAAGGAKTSAAWFVGMSKQIATAVMFVAGDEGTDNLNAYSSGFYGAGPPLSTWMA
                     YMSVAQSGLKDVSFDPPTQRTSTQRPTAPPRTQQPTTSRPTQTATTKPTETATSEPTT
                     QPTGEPTDEPTEKPTEKPTAKPTATQT"
     gene            1605..2960
                     /locus_tag="PLF56_08195"
     CDS             1605..2960
                     /locus_tag="PLF56_08195"
                     /inference="COORDINATES: protein motif:HMM:NF021134.2"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_function: GO:0016758 - hexosyltransferase activity
                     [Evidence IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="glycosyltransferase 87 family protein"
                     /protein_id="HOQ53580.1"
                     /translation="MTARTAFGSGVGRRAERAGVWFNPQPWVLLAGTCSFLVLMLRQL
                     PCALGAPVYKAMCYSDILPLFYARGLSEGKVPFITAEVEYPVLTGFLMDFARRITMAL
                     GGQVGADLPDSVVTHAANLFVGVNAVLLFAFFMLVLWAHLRLSRPWDAMMIAVSPAVI
                     TTGLINWDFMVVALTALGVLAWSRKRPVWAGVWLGLGVAAKLYPLLLFVPLAVLCFRS
                     GRLRQFWLALAGGVASWVAVNLPIYLLSPSGWLYFWTFNVDRGADWGSLWYALSLAGV
                     QVDDVSRAQSVCMVLGTAAICALLLLAPRRPRFAQGVLLLLVLFLVVNKVYSPQYVLW
                     LLPFLVLARPRWRDWAVFGVSETLYFMAIWGQLDGTLSTGAGGSGPYMAAVFLRIGVQ
                     LWIAALVIRDIVRPEFDPVRNPRWNSRHALSEPLLDDPDGGVLDRAPDASWMRVVPWL
                     R"
     gene            2948..3514
                     /locus_tag="PLF56_08200"
                     /pseudo
     CDS             2948..3514
                     /locus_tag="PLF56_08200"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_008598656.1"
                     /note="incomplete; partial in the middle of a contig;
                     missing C-terminus; Derived by automated computational
                     analysis using gene prediction method: Protein Homology.
                     GO_function: GO:0000009 - alpha-1,6-mannosyltransferase
                     activity [Evidence IEA];
                     GO_function: GO:0004376 - glycolipid mannosyltransferase
                     activity [Evidence IEA];
                     GO_process: GO:0006506 - GPI anchor biosynthetic process
                     [Evidence IEA]"
                     /pseudo
                     /codon_start=1
                     /transl_table=11
                     /product="mannosyltransferase family protein"
BASE COUNT          604 a         1525 c         1648 g          770 t
ORIGIN      
        1 ggctacctca acaccgtcta cttcggacgc ggcgcctacg gcatccaggc cgccgcgcag
       61 gcctacttcg gcaagcccgc cgcgaagctg accgacgccc aggccatcgc gctcacgtcg
      121 atcgtgaaca acccgggcaa cctcgacccg gccaagggcg acaaacaggc cgccgacctg
      181 ctggagcgct accagtacac gatcaacggc atggtcgaga tgggcaccat gaccgaggcc
      241 cagaaggccg agatctacta ccagctgccc aagtttccca aggtcgagcg cgacagccag
      301 tacggcggcc ccaagggatt cctgctcaac atggtgcagg acgagctgaa ggccaagggc
      361 ttcagcgacg agcagatcgc cggcggtggg ctgaccatcc gcaccacctt cgacgtcaag
      421 gcgcaggagg cggcggtcga ggcggcgcag aaggtgaccg cccagaccgc ggcggccggt
      481 aacaagaagg cctcgaacct gcacgctggg ctggcctccg tcgatgtcgc caccggcggc
      541 gtgctggccc tctacggggg ccccgacttc gtcaagaaca gccgcaactg ggcgaccacc
      601 ccccgtccga ccggctcgac gttcaagccc tacgcgctgg gcgccgcgct cgctcagggg
      661 ttcaccctca acgacacgct caacgggtcg cagttcgttc cgccgggcgc cgccaggccc
      721 gtgcgcaacg ccggcgaccg caactacggc acggtcacgc tgcagcaggc caccacccat
      781 tcgatcaaca ccgccttcgt cgatctcgag acccagatga aggacggtcc cgagtcgacc
      841 ctcgcgttcg cccaggcggc gggcctgccc aagacggccg gctgggaccc caccgtggtg
      901 gtgccgctgg gcgtgcccga ggtcagtccg ctcggccagg ccaccgggtt cgccacgttc
      961 gcgcgcggcg gcgagctgat cgagacccac gtggtcgccg aggtcaagaa cgcctccggt
     1021 gccacgctgt tcaaggccga cacctcggcc gagcagacca tcgaggccga cgtggccaac
     1081 gacgtcgcct atgcgctcac ccacgtggtg caggacggca ccgcctcacg ggccggcggc
     1141 atgggctttc cgatcgccgg caagaccggc acctactacc tgcgtgacgc ggccggtggc
     1201 gccaagacca gcgccgcctg gttcgtcggc atgagcaagc agatcgcgac cgccgtcatg
     1261 ttcgtggccg gcgatgaggg cacggacaac ctgaacgcct actcgtccgg cttttacggc
     1321 gccgggccgc cgctgagcac ctggatggcc tacatgtcgg tggcgcagag cggcttgaag
     1381 gacgtttcgt tcgatccgcc cacgcagcgc acctcgaccc agcgcccgac ggccccgccg
     1441 cgcacgcagc agcccaccac gtccaggccg acccagaccg ccaccaccaa gcccaccgaa
     1501 accgcgacgt ccgagccgac cacccagccc accggcgaac caacggacga gccgaccgag
     1561 aagcccacgg agaagccgac agccaagccg acggcgaccc agacgtgacc gcgcgcacgg
     1621 ccttcggctc aggtgtgggc cgccgggccg aacgcgccgg ggtgtggttc aacccgcaac
     1681 cctgggtgtt gttggccggc acgtgttcgt ttctggtgct gatgctgcgg caactgccct
     1741 gcgcgctcgg tgccccggtg tacaaggcga tgtgctacag cgacattctg ccgttgttct
     1801 atgcgcgcgg gctgtccgag ggcaaggtgc cgttcatcac cgctgaggtc gagtacccgg
     1861 tgctgaccgg ctttctgatg gacttcgccc gacgcatcac gatggccctg ggtgggcagg
     1921 tcggggccga cctgcccgac tcggtggtca cccatgccgc caacctgttc gtcggcgtga
     1981 acgcagtgct gctgttcgcc ttcttcatgc tcgtgctgtg ggcacatctg cgcttgtcac
     2041 ggccctggga cgcgatgatg atcgcggtct cgccggcggt gatcaccacc ggcctgatca
     2101 actgggactt catggtggtg gcgctcaccg cactgggggt gctggcgtgg tcgcgcaagc
     2161 gtccggtgtg ggccggggtg tggctggggc tcggggtggc ggccaagctc tacccgctgc
     2221 tgttgttcgt gccgctggcg gtgttgtgct ttcgttcggg caggctgcgg cagttctggc
     2281 tggcgctggc cggcggcgtg gccagttggg tggcggtgaa cctgccgatc tatctgctca
     2341 gcccgtcggg gtggctctac ttctggacct tcaacgtcga ccggggcgcc gattggggct
     2401 cgttgtggta cgcgctcagc ctggccgggg tgcaggtcga cgacgtgtcg cgggcgcaga
     2461 gcgtatgcat ggtgctcggc acggcggcga tctgtgcgct gctgctgctg gcgccacgcc
     2521 ggccccgctt cgcccagggt gtgctgctgc tgctggtgct gtttctggtg gtcaacaagg
     2581 tgtactcgcc gcagtacgtg ctgtggctgc tgccgtttct ggtgctggcc cggccgcgct
     2641 ggcgcgactg ggcggtgttc ggcgtctcgg agaccctgta cttcatggcc atctggggcc
     2701 agctggacgg caccctgtcc accggcgcgg gcggctcggg gccctatatg gcggcggtct
     2761 tcctccgcat cggggtgcag ttgtggatcg ccgcgctggt gatccgcgac atcgtccggc
     2821 ccgagttcga ccccgtccgc aacccccggt ggaacagccg gcatgcgctg agcgagccgc
     2881 tgctcgacga tccggacgga ggcgtgctcg accgggcacc ggacgcgtcc tggatgcggg
     2941 tcgtgccatg gctgcgctga cgttcgcccc gccgtcccga ccgctggtgg gggtgcgcga
     3001 cggcagccgg ctggtggtgc agacctggct gatcacgcga ctgctgctgg tggcggtggc
     3061 gctgtgggtg ggcaggacga cgggacgctc ggcgtccgac atgctcgcca actgggacgt
     3121 gcagcactac ttcgagatcg ccaagcacgg ctatgccgcc gacaacgaca tcgcgttctt
     3181 tcccggctgg ccgctggtgt tgcgggcgtt cgccagcctc ggtgtgccga tgctctgggc
     3241 cggggtgggc ctggcgatga tctgctcggc gttcgcggcg gcggcgctgt accggctggg
     3301 cgggccgatc gcggcggtgg tgtggctgct ggcccccacc acggtattca ccatggtgcc
     3361 ctacaccgag gcgccgttct gcgcggcggc cttctgggcg tgggaacgcg cccgagccgg
     3421 acgatggggt gccgccgccg gcctggccgc gatcgcctgc acgctgcggg tctcagggct
     3481 gtttctgctc ggcgccctgg tgatcctggc gttgacgtcg cgccgctcgg gtggatggcc
     3541 gggcgtgcat cagggcggtc agggcgctca aggttggacg agcgcgtccg gccagcccgg
     3601 tgtggttgct ggctcgcgtg cgagggcagg ggcaccctcc ggtcggagtt ggctgtcgtg
     3661 gtggccttgg cccgggcgac gcgtggacgc ggctgcgtcc ggtcaggacg gcgaggggcg
     3721 gggctggttc gcgtcgcgag gagtcgaccc gggcggcgtc ggccggggcg aggtctcccc
     3781 gcgaggaatc gcggggggtg gtttctcggg gggtggtttc tcgtggggtg gtgccgctca
     3841 gtctggtttt ccagcggacc gacgcacgtc cggcgggcag gggaacggtt cgtccaccga
     3901 cggggcagga ccgctggcgc gggtcgcggc gtccctgagg ttgtggtgga cgggtgatcg
     3961 ggcgcgagct ctcgcatggt tgagcattcc ggcggcgacg ctgttcgcct acatgttcta
     4021 cctgtaccgg ctgaccggcg actggaacgc ctggtaccgc gcgcagtcga ccggctggcc
     4081 gcgcggcttc acctggccgt gggtgtcgct gcaacacacc ctcgaagcgg ccgcgtcgaa
     4141 ggcgtacccc gcatttccgg aatggtcgtg ggtgttccgg gccgagttgg cggcgatggc
     4201 gatcggagtg atcgtgaccc tgatctgcct gcgcaagcac cgttgggccg aggcggcctg
     4261 ggtgggtgtt caggtgctcg ccttctcgtg ctcgtactgg tatctgagcg tgacccgtgc
     4321 ggtcctgctg tggtttccgc tgttcgtgct gctcggcgag cgactctcgg gcggctggac
     4381 cctgacgccc ggccggcgcc ggctgcgcat ggtcgtgacg gccatcgcaa tcgctgcggc
     4441 cgggtacgtg ctgtgctggt ggggctggct gttcttcacc ggcaggtggg caagctagcg
     4501 cacgcttgag cgcctcgtgc ggcttcccag gtcatggtgg atcggag
//
LOCUS       DAOSUG010000224         4537 bp    DNA     linear   ENV 10-JUN-2024
DEFINITION  MAG TPA_asm: Micropruina sp. isolate AMR_MDS_2195
            NODE_8199_length_4537_cov_4.003570, whole genome shotgun sequence.
ACCESSION   DAOSUG010000224 DAOSUG010000000
VERSION     DAOSUG010000224.1
DBLINK      BioProject: PRJNA925477
            BioSample: SAMN32864262
            Sequence Read Archive: SRR4236673
KEYWORDS    WGS; ENV; Metagenome Assembled Genome; MAG; Third Party Data; TPA;
            TPA:assembly.
SOURCE      Micropruina sp. (wastewater metagenome)
  ORGANISM  Micropruina sp.
            Bacteria; Bacillati; Actinomycetota; Actinomycetes;
            Propionibacteriales; Nocardioidaceae; Micropruina.
REFERENCE   1  (bases 1 to 4537)
  AUTHORS   Abdulkadir,N., Saraiva,J.P., Zhang,J., Stolte,S., Gillor,O.,
            Harms,H. and Rocha,U.
  TITLE     Genome-centric analyses of 165 metagenomes show that mobile genetic
            elements are crucial for the transmission of antimicrobial
            resistance genes to pathogens in activated sludge and wastewater
  JOURNAL   Microbiol Spectr 12 (3), e0291823 (2024)
   PUBMED   38289113
REFERENCE   2  (bases 1 to 4537)
  AUTHORS   Abdulkadir,N.
  TITLE     Direct Submission
  JOURNAL   Submitted (24-MAR-2023) Environmental Microbiology, Helmholtz
            Center for Environmental Research -UFZ, Permoserstr 15, Leipzig
            04318, Germany
COMMENT     The annotation was added by the NCBI Prokaryotic Genome Annotation
            Pipeline (PGAP). Information about PGAP can be found here:
            https://www.ncbi.nlm.nih.gov/genome/annotation_prok/
            
            ##Genome-Assembly-Data-START##
            Assembly Method        :: SPAdes v. 3.11.1
            Genome Representation  :: Full
            Expected Final Version :: Yes
            Genome Coverage        :: Not Applicable
            Sequencing Technology  :: Illumina MiSeq
            ##Genome-Assembly-Data-END##
            
            ##Genome-Annotation-Data-START##
            Annotation Provider               :: NCBI
            Annotation Date                   :: 05/04/2023 04:57:10
            Annotation Pipeline               :: NCBI Prokaryotic Genome
                                                 Annotation Pipeline (PGAP)
            Annotation Method                 :: Best-placed reference protein
                                                 set; GeneMarkS-2+
            Annotation Software revision      :: 6.5
            Features Annotated                :: Gene; CDS; rRNA; tRNA; ncRNA
            Genes (total)                     :: 2,776
            CDSs (total)                      :: 2,750
            Genes (coding)                    :: 2,714
            CDSs (with protein)               :: 2,714
            Genes (RNA)                       :: 26
            tRNAs                             :: 24
            ncRNAs                            :: 2
            Pseudo Genes (total)              :: 36
            CDSs (without protein)            :: 36
            Pseudo Genes (ambiguous residues) :: 0 of 36
            Pseudo Genes (frameshifted)       :: 2 of 36
            Pseudo Genes (incomplete)         :: 32 of 36
            Pseudo Genes (internal stop)      :: 2 of 36
            ##Genome-Annotation-Data-END##
FEATURES             Location/Qualifiers
     source          1..4537
                     /organism="Micropruina sp."
                     /mol_type="genomic DNA"
                     /submitter_seqid="NODE_8199_length_4537_cov_4.003570"
                     /isolate="AMR_MDS_2195"
                     /isolation_source="Activated sludge of municipal
                     wastewater treatment plants"
                     /db_xref="taxon:2737536"
                     /environmental_sample
                     /geo_loc_name="Canada"
                     /lat_lon="49.25 N 123.1 W"
                     /collection_date="2015-06-16"
                     /metagenome_source="wastewater metagenome"
                     /note="metagenomic"
     gene            <1..848
                     /locus_tag="PLF56_08205"
     CDS             <1..848
                     /locus_tag="PLF56_08205"
                     /inference="COORDINATES: protein motif:HMM:NF014893.2"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_function: GO:0016491 - oxidoreductase activity
                     [Evidence IEA];
                     GO_function: GO:0016651 - oxidoreductase activity, acting
                     on NAD(P)H [Evidence IEA]"
                     /codon_start=3
                     /transl_table=11
                     /product="Gfo/Idh/MocA family oxidoreductase"
                     /protein_id="HOQ53581.1"
                     /translation="SRSSQRAESFATEHRIPRWHASYDSLVNDPAVDAIYIATPHPQH
                     TDIALAAIDAGKSILVEKAFTATVEDTERIVTAARQAKVFAMEAMWTRFNPAIAHLRE
                     AIASGELGEIRGCQGDLTAYREYDPSDRLFDPAKGGGAVLDLGVYVVSFAQHFLGTPE
                     VVRAAGGRYASGVEGEFAVLFGYPDGRSATLAGAFTTYGPGRIMLTGTKGWVDVHPRF
                     HRSASVTIWRGKQPETLEFESGYRFEIEHVGECLAQGLTESPIMPLGDTIAVQAILAE
                     VLSQI"
     gene            complement(898..1710)
                     /locus_tag="PLF56_08210"
     CDS             complement(898..1710)
                     /locus_tag="PLF56_08210"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_013864154.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_function: GO:0008168 - methyltransferase activity
                     [Evidence IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="methyltransferase domain-containing protein"
                     /protein_id="HOQ53582.1"
                     /translation="MTIPLPQSERRIEDVQGHWLLARLGKRVLRPGGLELTNQLLAHV
                     RLQGAEVVELAPGIGRTAALIVQAGPAGYVGVDADPSAAALVAEVVGDAGRTITADAA
                     QTGLPEASADVVIGEAMLTMQTERGKKAIIDEAVRVLRPGGRYAVHELGLQPDDLDPA
                     IGDDIRKALARSIKANARPLTAAEWTALLTDSGLEVEWVGTAPMALLQLRRNLADEGL
                     RRVVRIVWNVLRDPGARKRVLAMRRTFTQYADHLTGIALVARKPRDPGTSLV"
     gene            complement(1741..2052)
                     /locus_tag="PLF56_08215"
     CDS             complement(1741..2052)
                     /locus_tag="PLF56_08215"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_003795534.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="cupin domain-containing protein"
                     /protein_id="HOQ53583.1"
                     /translation="MTDLSDLASLVQVQPQSTVSRTVLKAEGTNVVLFAFDTDEELRE
                     HTAAIPVLLQTLEGAFEITADGRTVTLRPGDLIHLRTRLPHAVKALEPSKLALFLLTN
                     D"
     gene            2122..2817
                     /locus_tag="PLF56_08220"
     CDS             2122..2817
                     /locus_tag="PLF56_08220"
                     /inference="COORDINATES: protein motif:HMM:NF013209.2"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_function: GO:0003677 - DNA binding [Evidence IEA];
                     GO_process: GO:0006355 - regulation of DNA-templated
                     transcription [Evidence IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="helix-turn-helix domain-containing protein"
                     /protein_id="HOQ53584.1"
                     /translation="MHNAPPLGPRPARTDRPSEAALRVLRVLASGGEPLTSAELTAEL
                     GGHPNTVRPHLDRLVAEGFVDIVALPAVGRGRPARAYRATIAGRQVAGQDHSTLAGAA
                     LLGAITDELRGTAEPAFAARRLGRSWGRRLGAGSRRQAGQPDLAAGHEVGPIVTALSA
                     LAGQGFTPQPEPDGLHLLTCPMLAEAREAPEVVCSLHQGMLDALSDAPLRLVPFAGPG
                     YCLVSPVTDDDRS"
     regulatory      2851..2956
                     /regulatory_class="riboswitch"
                     /inference="COORDINATES: nucleotide
                     motif:Rfam:14.4:RF00059"
                     /inference="COORDINATES: profile:INFERNAL:1.1.4"
                     /note="TPP riboswitch; Derived by automated computational
                     analysis using gene prediction method: cmsearch."
                     /bound_moiety="thiamine pyrophosphate"
                     /db_xref="RFAM:RF00059"
     gene            2993..4102
                     /locus_tag="PLF56_08225"
     CDS             2993..4102
                     /locus_tag="PLF56_08225"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_015071490.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="thiamine ABC transporter substrate-binding
                     protein"
                     /protein_id="HOQ53585.1"
                     /translation="MSTRNHRRRALRALAALAGLALFAGCAGGSGATGPADGTPSTGA
                     STTAPTESKKLTVVTHDSFALSDELKKQFADETGYTVTYVAPGDGGALVNQLILTKDS
                     PLGDVVYGIDNTFAGRAVKAGVLTPYTPADLPASVADLKADDSGSLTAVDFGDVCVNA
                     DKNWFKAKNLPLPVTLDDLAKPEYKNLLVVENPASSSPGLAWLAATVGAKGDPGYLDY
                     WTALKNNGVRVVKGWTEAYSTEFSGSSGKGSRPLVVSYSTSPAFEVPKGSDQSNTTAL
                     LQTCFRQVEYAGVIAGAQNQVGAQKFIDFMLSKKVQADIPGEMYMYPADTSVALPKEW
                     VAHAPLSSKPFTVPADVIAASRDTWIRDWTAKVIG"
BASE COUNT          689 a         1527 c         1592 g          729 t
ORIGIN      
        1 gctcgcgttc gtcgcagcgc gcggaatcgt tcgccaccga gcacaggatt ccccgctggc
       61 atgcgtcgta tgactcgttg gtcaacgacc ctgcggtcga cgcgatctat atcgccacgc
      121 cgcacccgca gcacaccgac atcgcgctgg ctgcgatcga tgcgggcaag tcgattctgg
      181 tcgagaaggc gttcacggcc acggtcgaag acactgagcg catcgtgacc gccgcacggc
      241 aggcgaaggt tttcgccatg gaggcgatgt ggacgcggtt caaccccgcc atcgcccatc
      301 tgcgcgaggc gatcgcatcg ggcgagctcg gcgagatccg cgggtgccag ggcgacctga
      361 ccgcgtatcg cgagtacgac ccgtccgatc gcctcttcga tccggccaag ggcggcgggg
      421 cggtgctcga cctgggcgtg tacgtggtgt cgttcgcgca gcactttctc ggcactcccg
      481 aggtggtgcg tgcggccggt gggcgctatg ccagcggagt ggagggcgag ttcgcggtac
      541 tgttcggcta cccggacggc cgttccgcca cgctcgccgg cgccttcacc acctatggcc
      601 ccggacgcat catgctcacc ggcaccaagg gctgggtgga cgtgcacccg cggttccacc
      661 gcagcgcctc ggtgacgatc tggcgcggca agcagcccga gacgctcgag ttcgaatcgg
      721 gctaccggtt cgagatcgaa cacgtcggcg agtgcctcgc ccaagggctg acggagagcc
      781 cgatcatgcc actgggcgac acgatcgccg tgcaggcgat cctggccgag gtgctgtccc
      841 agatctaggt ggacagcacg cactagggga aacgctgatc aatgcccggc gtactcgtca
      901 taccaggctt gtgccgggat cccggggttt gcgggccacc aaggcgatgc cggtcaggtg
      961 gtcggcgtac tgcgtgaacg tgcggcgcat ggcgagcacc cgcttgcggg cgcccgggtc
     1021 gcgcaacaca ttccagacga ttcgaaccac ccggcgaaga ccctcgtcgg ccaggttgcg
     1081 gcgcaactgc agcagcgcca tcggcgcggt gcccacccat tcgacctcca ggccggagtc
     1141 ggtcagcagc gccgtccatt cggcggcggt caggggccgg gcgttggcct tgatcgagcg
     1201 ggccagcgcc ttgcggatgt cgtctccgat ggctgggtcc aggtcgtcag gctggagccc
     1261 gagttcatgg acggcgtagc gtccgcccgg cctcagtact cgcacggcct cgtcgatgat
     1321 cgccttcttg ccgcgctcgg tctgcatggt cagcatggcc tcgccgatca ccacgtccgc
     1381 ggaggcctcg ggcaggccgg tctgtgcggc atcggcggtg atcgtgcgtc cggcatcgcc
     1441 gaccacctcg gcgaccaggg ccgcggcgga cgggtcggcg tccacgccga cgtagccggc
     1501 cggtccggcc tgcacgatga gcgcggccgt ccgtccgatg ccgggggcca gctcgaccac
     1561 ctctgcaccc tgcagccgga cgtgcgccag cagctgattg gtcagttcca gtccgcccgg
     1621 gcggagcacg cgtttgccca ggcgggccag cagccagtga ccctggacgt cttcgatccg
     1681 gcgttcggac tggggtaggg gaatggtcat cgtcgctctc gattcaggtg ggaggggcgc
     1741 tcagtcgttg gtcagcagaa acagcgcgag cttggacggc tccagcgcct tgaccgcatg
     1801 aggcaagcgg gtccgcaggt ggatcaggtc gcccggacgc agggtgaccg tgcgtccgtc
     1861 cgcggtgatc tcgaaggcgc cttcgagggt ctgcagcagc accgggatcg cggcggtgtg
     1921 ctcgcgcaat tcttcgtcgg tgtcgaacgc gaacaacacc acgttggtgc cttcggcctt
     1981 cagtaccgtg cgggacacgg ttgattgcgg ttgcacctgc accagcgaag ccaggtcgga
     2041 caggtcggtc atctgcgagt cgctcacgac tactcctatt taatctatgt tgatatgtac
     2101 aaaatacagt aggctgtccg catgcacaac gcccccccgc tgggacctcg gccggcgcgc
     2161 accgaccgtc cgtccgaggc ggccttgcgc gtactgcgtg tgctggcctc cggcggcgag
     2221 ccgctcacca gcgcggaact gaccgccgag ttgggcgggc accccaacac cgtccggccg
     2281 cacctggacc ggttggtggc cgagggcttc gtcgacatcg tggccctgcc cgccgtcggt
     2341 cgtggacggc cggcgcgcgc ctaccgtgcc accatcgccg gacgccaggt ggccggccag
     2401 gaccactcga cgctggccgg cgccgccctg ctgggtgcaa tcaccgacga gttgcgcggt
     2461 accgccgaac ccgcgttcgc cgcccgccgg ctcggacgat cctggggccg ccggctgggt
     2521 gcgggatcgc ggcggcaagc ggggcagccg gacctggcgg cggggcacga ggtggggccg
     2581 atcgtgaccg cgctgagtgc cctcgccggc cagggattca ccccccaacc cgagccggac
     2641 ggactgcacc tgctgacctg cccgatgctg gccgaggctc gcgaagcgcc ggaggtggtc
     2701 tgcagtctgc atcagggcat gctcgatgcg ctgtcggacg ctccgctgcg gctggtgccc
     2761 ttcgccgggc cggggtattg cctggtgagt ccggtcacgg acgacgaccg cagctgagct
     2821 gaggccccgg ggcgtccgac gggtagagtg ggtcctcgac aggggagcgc caccacggcg
     2881 ctgagagtgc ggcaggccgc agaccctcga acctgacccg gttagcaccg gcgtagggag
     2941 tcgagtcatc tcctcaccac agggtgagcc ctccgataag gaggaaccga acatgtccac
     3001 acgcaaccat cgacgccgag ccctgagggc tttggccgcc ctggcgggtc tcgcactctt
     3061 cgcgggttgt gccggcggtt ccggtgccac cgggccggcc gacggcacgc cgtccacggg
     3121 tgcatccacc acggcgccca cggagtcgaa gaagctgacc gtggtcaccc atgactcgtt
     3181 cgcgctcagc gacgagctca agaaacagtt cgcggacgag accgggtaca ccgtcaccta
     3241 cgtggcgccc ggcgacggtg gcgccctggt gaaccagctg atcctgacca aggattcgcc
     3301 gctgggtgac gtggtgtacg gcatcgacaa cacgttcgcc gggcgggcgg tcaaggcggg
     3361 ggtgctgacc ccctacacgc cggccgacct acccgcctcg gtggccgacc tcaaggcgga
     3421 cgactccggc tcgctcacgg cggtcgactt cggcgacgtg tgcgtgaacg ccgacaagaa
     3481 ctggttcaag gccaagaacc tgccgctgcc ggtgaccctg gacgacctcg ccaagcccga
     3541 gtacaagaac ctgctggtgg tcgagaaccc ggcatcgtcg tcgcccgggc tggcctggct
     3601 ggcggccact gtgggcgcca agggcgaccc cggctacctc gattactgga ccgcgctgaa
     3661 gaacaacggc gtccgcgtgg tcaagggctg gacggaggcc tactccaccg agttctccgg
     3721 gtcgtcgggc aaggggtcgc ggccgctggt ggtgtcgtac agcacgtccc cggcgttcga
     3781 ggtgcccaag ggctccgacc agtcgaacac caccgcgttg ctgcagacct gctttcggca
     3841 ggtggagtac gccggggtga tcgccggcgc gcagaaccag gtcggtgcgc agaagttcat
     3901 cgacttcatg ctgagtaaga aggtgcaggc cgacattccg ggtgagatgt acatgtaccc
     3961 ggccgacacc tcggtcgcgt tgcccaagga atgggtggcg cacgcacccc tgtcgagcaa
     4021 gccgttcacg gtgccggccg atgtaatcgc ggcttcgcgt gacacctgga tccgcgactg
     4081 gaccgcgaag gtgatcgggt aagcgatgag accctcggtc gagcgtggac gagcggacgt
     4141 gcgtccgtcg ctcgagcgcg ggtcggtcgc gggttcgtcg gtcgagggtt cggcggtcga
     4201 accttcgccg cccgcgtctt cgtcacccaa gtgttcggcg gtcgaacctt cgtgggtcga
     4261 gcttgtcgag accgcgtcgt cggggtccac gtcgccccgg tcttcgtcgg tcgagcctgt
     4321 cgagacggcg gtgtctggaa caccgtcggt cggaacacga cgctcctcga gcacgaccac
     4381 ggggtctggt caagctcgac cagcgcatcg ggctcgaccg agggatgggg ttcgaccagc
     4441 ggatggggct cgaccggggg aggggcctcg accagctcgc atcccactcg ctgccgtcct
     4501 gttctgggcc ctggccgtgc tgctcccggt cggcttt
//
LOCUS       DAOSUG010000225         4528 bp    DNA     linear   ENV 10-JUN-2024
DEFINITION  MAG TPA_asm: Micropruina sp. isolate AMR_MDS_2195
            NODE_8216_length_4528_cov_4.071093, whole genome shotgun sequence.
ACCESSION   DAOSUG010000225 DAOSUG010000000
VERSION     DAOSUG010000225.1
DBLINK      BioProject: PRJNA925477
            BioSample: SAMN32864262
            Sequence Read Archive: SRR4236673
KEYWORDS    WGS; ENV; Metagenome Assembled Genome; MAG; Third Party Data; TPA;
            TPA:assembly.
SOURCE      Micropruina sp. (wastewater metagenome)
  ORGANISM  Micropruina sp.
            Bacteria; Bacillati; Actinomycetota; Actinomycetes;
            Propionibacteriales; Nocardioidaceae; Micropruina.
REFERENCE   1  (bases 1 to 4528)
  AUTHORS   Abdulkadir,N., Saraiva,J.P., Zhang,J., Stolte,S., Gillor,O.,
            Harms,H. and Rocha,U.
  TITLE     Genome-centric analyses of 165 metagenomes show that mobile genetic
            elements are crucial for the transmission of antimicrobial
            resistance genes to pathogens in activated sludge and wastewater
  JOURNAL   Microbiol Spectr 12 (3), e0291823 (2024)
   PUBMED   38289113
REFERENCE   2  (bases 1 to 4528)
  AUTHORS   Abdulkadir,N.
  TITLE     Direct Submission
  JOURNAL   Submitted (24-MAR-2023) Environmental Microbiology, Helmholtz
            Center for Environmental Research -UFZ, Permoserstr 15, Leipzig
            04318, Germany
COMMENT     The annotation was added by the NCBI Prokaryotic Genome Annotation
            Pipeline (PGAP). Information about PGAP can be found here:
            https://www.ncbi.nlm.nih.gov/genome/annotation_prok/
            
            ##Genome-Assembly-Data-START##
            Assembly Method        :: SPAdes v. 3.11.1
            Genome Representation  :: Full
            Expected Final Version :: Yes
            Genome Coverage        :: Not Applicable
            Sequencing Technology  :: Illumina MiSeq
            ##Genome-Assembly-Data-END##
            
            ##Genome-Annotation-Data-START##
            Annotation Provider               :: NCBI
            Annotation Date                   :: 05/04/2023 04:57:10
            Annotation Pipeline               :: NCBI Prokaryotic Genome
                                                 Annotation Pipeline (PGAP)
            Annotation Method                 :: Best-placed reference protein
                                                 set; GeneMarkS-2+
            Annotation Software revision      :: 6.5
            Features Annotated                :: Gene; CDS; rRNA; tRNA; ncRNA
            Genes (total)                     :: 2,776
            CDSs (total)                      :: 2,750
            Genes (coding)                    :: 2,714
            CDSs (with protein)               :: 2,714
            Genes (RNA)                       :: 26
            tRNAs                             :: 24
            ncRNAs                            :: 2
            Pseudo Genes (total)              :: 36
            CDSs (without protein)            :: 36
            Pseudo Genes (ambiguous residues) :: 0 of 36
            Pseudo Genes (frameshifted)       :: 2 of 36
            Pseudo Genes (incomplete)         :: 32 of 36
            Pseudo Genes (internal stop)      :: 2 of 36
            ##Genome-Annotation-Data-END##
FEATURES             Location/Qualifiers
     source          1..4528
                     /organism="Micropruina sp."
                     /mol_type="genomic DNA"
                     /submitter_seqid="NODE_8216_length_4528_cov_4.071093"
                     /isolate="AMR_MDS_2195"
                     /isolation_source="Activated sludge of municipal
                     wastewater treatment plants"
                     /db_xref="taxon:2737536"
                     /environmental_sample
                     /geo_loc_name="Canada"
                     /lat_lon="49.25 N 123.1 W"
                     /collection_date="2015-06-16"
                     /metagenome_source="wastewater metagenome"
                     /note="metagenomic"
     gene            complement(<1..191)
                     /locus_tag="PLF56_08230"
     CDS             complement(<1..191)
                     /locus_tag="PLF56_08230"
                     /inference="COORDINATES: ab initio
                     prediction:GeneMarkS-2+"
                     /note="Derived by automated computational analysis using
                     gene prediction method: GeneMarkS-2+."
                     /codon_start=1
                     /transl_table=11
                     /product="hypothetical protein"
                     /protein_id="HOQ53586.1"
                     /translation="MMPPALRLVPSNPMNRVLMAVLCFEAICCGLAIPGMIQVSSVSL
                     SLAFTTGGAALLLCIAAAGT"
     gene            complement(205..1539)
                     /locus_tag="PLF56_08235"
     CDS             complement(205..1539)
                     /locus_tag="PLF56_08235"
                     /EC_number="6.3.2.-"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_011756903.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_function: GO:0004326 - tetrahydrofolylpolyglutamate
                     synthase activity [Evidence IEA];
                     GO_function: GO:0005524 - ATP binding [Evidence IEA];
                     GO_function: GO:0008841 - dihydrofolate synthase activity
                     [Evidence IEA];
                     GO_function: GO:0046872 - metal ion binding [Evidence
                     IEA];
                     GO_process: GO:0006730 - one-carbon metabolic process
                     [Evidence IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="folylpolyglutamate synthase/dihydrofolate
                     synthase family protein"
                     /protein_id="HOQ53587.1"
                     /translation="MPQTHQQIVQQLIARWPEHRIAPGTARVEALCELLGSPQRGYPV
                     IQIAGTNGKGSTAIMIDALLRALGLRTGRYTSPHLIELTERISIDGEPISDESFDALV
                     ADTAPLVDLVDERAIDGVPMTFFEVMTALAYEAFAQAPVDVAVVEVGLGGVTDATNVM
                     DAAVAVICPIDFDHTHLLGTSLTEIAREKAGIIKAGSQVVLAAQQPEAAAVLVERCAE
                     AGAKAYLEGIDFGVLERTPGVGGQVLRLNTAGGAADDVVLPLFGAHMAHNAALAVAAV
                     EAFLGGKPLPADVIAEGLGAVVAPGRLEIVRRSPTVVLDTCHNPHGARATMDALTETF
                     AFTPLIGVVAVMADKDVDGLLRVFADEMSTIVCTTAAGTSRALPAQDLAERASGIVGD
                     ERVRIAASMADAIEMAVALADDAGPSAGVLIAGSVIAAGEARALLVRDRGSR"
     gene            1627..2157
                     /locus_tag="PLF56_08240"
     CDS             1627..2157
                     /locus_tag="PLF56_08240"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_004808759.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_component: GO:0016020 - membrane [Evidence IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="DoxX family protein"
                     /protein_id="HOQ53588.1"
                     /translation="MSSSVASRPWVRYVTFAARQILGIVLIVAGAVKLPWPEASVLAV
                     RAYQLLPFDLTRPVGYALPVIEVSIGLLLVLGLFTRLAAWLGALTMVAFIIGISWVWA
                     HGISIDCGCFGGGGESANGIAQYPWDIARDVGLMACGVWVGLFPRSPLALDNWLFGPS
                     DPPAPADDDEYEESRA"
     gene            2154..2933
                     /locus_tag="PLF56_08245"
     CDS             2154..2933
                     /locus_tag="PLF56_08245"
                     /inference="COORDINATES: protein motif:HMM:NF024853.2"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="thioredoxin domain-containing protein"
                     /protein_id="HOQ53589.1"
                     /translation="MTQSRREALRRQQEAQQRAKRLQRVIIVGAVVLAVVVAGVFATI
                     FFSQLNKGTGTPAAGGTSGVPVNATADSSGIVVNPGKFKDGVPKVEVFLDYQWPVCKQ
                     FEAKFGGTLDEMATKGEIQLIYRTMTFLDSNLRNDASTRAGVAAACADNVGAYSAYHN
                     AIFAGPPTTEGAGYTTEQLRVTFAATAGITGDKLATFQKCYDNRATGTFVQGTNEAAA
                     KAGVNSTPTIMVNGTKLDNNTLFQETTEAFKQTILTTKANG"
     gene            2926..3669
                     /locus_tag="PLF56_08250"
     CDS             2926..3669
                     /locus_tag="PLF56_08250"
                     /inference="COORDINATES: protein motif:HMM:NF024853.2"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="thioredoxin domain-containing protein"
                     /protein_id="HOQ53590.1"
                     /translation="MANSSSRERLRAAQQAETKRRRARVIAAIGVGVGALVAIAVMIW
                     ASMGGAAPSATTRPPNATASNDGIVVNPGKAKPNAPVVGLYFDYQCSHCVSFEEKYGL
                     TLNALSNNGDVSLVNHTRVFLDRGDATGLSHRAAIAAACADTAGVYKDYHQSIFSSAY
                     QGAYTDDLFRVTIPQQLGLSGDTLTRFQSCYDDRSMAGFVQGVDDAAAKAGVNSTPTL
                     MINGKTQNLTTLPEDVTKLKAFIEAAAKA"
     gene            3796..>4528
                     /locus_tag="PLF56_08255"
     CDS             3796..>4528
                     /locus_tag="PLF56_08255"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_014172534.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_function: GO:0000166 - nucleotide binding [Evidence
                     IEA];
                     GO_function: GO:0004812 - aminoacyl-tRNA ligase activity
                     [Evidence IEA];
                     GO_function: GO:0005524 - ATP binding [Evidence IEA];
                     GO_process: GO:0006418 - tRNA aminoacylation for protein
                     translation [Evidence IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="class I tRNA ligase family protein"
                     /protein_id="HOQ53591.1"
                     /translation="MSIASSSAASAPTPQGAVVTVPERPALEGLEAKWSQRWDEQQTY
                     AFERPAGREQVFSIDTPPPTVSGSLHVGHVFSYTHTDLVARYQRMQGKHVFYPMGWDD
                     NGLPTERRVQNFYGVRCDPSIAYDPDFTPPAKPDPKRQVSISRKNFIELCHELTAVDE
                     QAFERLWRTLGLSVDWRNHLYATISAESMRISQRAFLRNCARGEAYLAEAPTVWDVTF
                     QTAVAQAEQEARDYPGHYHRVAFHRP"
BASE COUNT          749 a         1625 c         1478 g          676 t
ORIGIN      
        1 gtgcccgccg ccgcgatgca gagcagcagc gccgcgccgc cggtggtgaa ggcgagcgac
       61 aacgacacgc tcgagacctg gatcatgccg gggatcgcca gcccgcagca gatcgcctcg
      121 aagcagagca cggccatcag cacccggttc atcgggttgc tcggcaccag tcgcagcgcc
      181 ggcggcatca tgctcggccc ggcctcatcg cgacccccgg tcacgcacca gtagcgcgcg
      241 ggcctcgccg gctgcgatca ccgaaccggc gatcagcacc ccggcgctcg ggcccgcgtc
      301 gtcggccagc gccacggcca tctcgatcgc atcggccatg gaggcggcga tccgcacccg
      361 ctcgtcgccc acgatgccgg acgcccgctc ggccaggtcc tgcgccggca gggcccgcga
      421 cgtgcccgcc gcggtggtgc agacgatcgt gctcatctcg tcggcgaaca cgcgcagcag
      481 gccgtccacg tccttgtcgg ccatcactgc taccacgccg atcagtgggg tgaacgcgaa
      541 ggtctcggtg agcgcgtcca tggtggcgcg tgccccgtgc gggttgtggc aggtgtcgag
      601 caccaccgtc ggcgaccgtc ggacgatctc gagccggccc ggcgcgacca ccgcgcccaa
      661 cccctcggcg atgacgtcgg cgggcagtgg cttgccgccc aggaacgcct cgaccgcggc
      721 taccgccagg gcggcgttgt gcgccatgtg cgcgccaaac aacggcagca ccacgtcgtc
      781 cgccgctccc cccgcggtgt tgagacgcag cacctgccca ccgacgccgg gagtgcgctc
      841 caacacgccg aaatcaatgc cctcgaggta ggccttggca ccggcttcgg cgcaacgttc
      901 gaccaggacg gccgcggcct cgggttgctg cgcggccagc accacctgcg agccggcctt
      961 gatgatgccc gccttctcgc gggcgatctc ggtcagcgag gtgcccagca ggtgggtgtg
     1021 gtcgaagtcg atcggacaga tcacggccac ggcggcgtcc atcacgttgg tggcgtcggt
     1081 gaccccgccc agcccgacct cgaccaccgc gacgtcgacc ggtgcctgtg cgaacgcctc
     1141 gtaggccagc gccgtcatca cctcgaaaaa ggtcatcggc accccgtcga tcgcgcgttc
     1201 gtccaccagg tcgaccaggg gcgcagtgtc ggcgaccagc gcatcgaagc tctcatcact
     1261 gatcggctcg ccgtcgatgc tgatgcgttc ggtcagctcg atcaggtgcg ggctggtgta
     1321 gcgaccggtg cgcagcccca gcgcgcgcag caacgcgtcg atcatgatgg cggtgctgcc
     1381 cttgccgttg gtgcccgcga tctgaatcac cgggtagccg cgctgcggcg aaccgagcaa
     1441 ctcgcacaac gcctcgaccc gcgcggtgcc gggcgcgatc cggtgctcgg gccagcgcgc
     1501 gatgagttgc tggacgatct gctgatgtgt ctgcggcatg gtccggtcag cctaccggtg
     1561 cggctagggc gcgtctccag acggcgacga caaagttgac gcgtcaaccg cagctacact
     1621 gctgccatgt ccagcagcgt tgccagccgt ccctgggttc gctacgtcac cttcgccgca
     1681 cggcagattc tgggcatcgt cctgatcgtc gccggggcgg taaagctgcc gtggcccgag
     1741 gcgtccgtgc tggctgtgcg cgcctaccag ttgctgccct tcgacctcac ccgtccggtc
     1801 ggctacgcgc tgccggtgat cgaggtgagc atcggcctgc tgctggtgct tggcctgttc
     1861 acccggctgg ccgcctggct gggcgcactc accatggtcg cgttcatcat cggcatctca
     1921 tgggtgtggg cgcacggcat ctcgatcgac tgcggctgct tcgggggtgg cggcgagagc
     1981 gccaacggca tcgcccagta cccttgggac atcgcacgcg acgtcgggct gatggcctgc
     2041 ggtgtctggg tcgggctgtt tccacgctcg ccgctggcac tcgacaactg gcttttcggc
     2101 ccgagcgacc cgccggcacc ggccgacgac gacgaatatg aggagtcccg agcatgaccc
     2161 agagccgacg tgaggcgctc cgtcgccagc aagaggcgca gcagcgcgcc aaacgcctgc
     2221 agcgggtgat catcgtgggg gccgtcgtgc tggccgtggt ggtggcgggc gtgttcgcca
     2281 ccattttctt cagccagctc aacaagggca cgggcacacc ggcggcaggc ggcacgtccg
     2341 gggtgccggt caatgccacc gccgacagca gcggcatcgt ggtgaacccg ggcaagttca
     2401 aggacggcgt gcccaaggtc gaggtctttc tcgactacca gtggccggtc tgcaaacagt
     2461 tcgaggccaa gttcgggggc accctcgacg agatggccac caagggcgag atccagctga
     2521 tctaccgcac catgaccttc ctcgacagca acctgcgcaa cgacgcctcg accagagccg
     2581 gcgtggcggc cgcgtgtgcc gacaacgtcg gtgcgtattc cgcctatcac aacgcgatct
     2641 tcgccggccc gccgaccacc gagggcgccg ggtacaccac cgagcagttg cgggtcacct
     2701 tcgccgccac cgcgggcatc accggtgaca agctggccac cttccagaag tgctacgaca
     2761 accgcgccac cggcaccttc gtgcagggca ccaatgaggc cgcggccaag gcgggggtca
     2821 actcgacgcc gaccatcatg gtcaacggca ccaagctcga caacaacacc ctgttccaag
     2881 agaccaccga agccttcaag cagaccatcc tgaccaccaa ggccaatggc taacagttcg
     2941 agtcgcgagc ggctgcgcgc cgcccaacag gcagagacca agcgtcgacg ggctcgcgtc
     3001 atcgccgcga tcggcgtcgg cgtcggggcc ctggtggcca tcgcggtgat gatctgggca
     3061 tccatgggcg gcgcagcccc gagtgccacc actcggcccc ccaacgcgac ggcgagcaat
     3121 gacggcatcg tggtgaaccc cggcaaggcc aaaccgaacg cgccggtagt cgggctctac
     3181 ttcgactacc agtgcagcca ctgcgtcagc ttcgaagaga agtacggcct caccctcaac
     3241 gcgttgtcga acaacggcga cgtctcgctg gtcaaccaca ccagggtctt tctcgaccga
     3301 ggcgatgcca ccggtttgtc gcaccgcgcg gccatcgcgg ccgcctgcgc cgataccgcc
     3361 ggggtctata aggactacca ccagagcatc ttcagctcgg cctaccaggg cgcctacacc
     3421 gacgatctgt tccgggtcac gattccgcag cagctggggc tcagcggcga tacgctgacc
     3481 cgctttcagt cctgctacga cgatcgctcg atggccggct tcgtccaggg cgtggacgac
     3541 gcggcggcca aggccggcgt caacagcact cccacgctga tgatcaacgg caagacccag
     3601 aacctgacca cgcttcccga ggacgtgacc aagctcaagg cattcatcga ggccgcggcc
     3661 aaggcctgag cccggatcca ccgatccggg ctgagccccg ggcgatgggc agccggccga
     3721 tgggcagccg gcgacggcgc cgtcccgctc ccgccgattg gtgggggcgc gacccgcttt
     3781 cgtaagatgc agcgcatgag cattgcatcg agttcggccg cctccgcccc caccccgcag
     3841 ggcgcggtcg tcacggtgcc cgagcgtccg gccctggagg ggctggaggc caaatggtcg
     3901 cagcgctggg acgagcagca gacctacgcc ttcgagcgtc cggccggccg cgagcaggtg
     3961 ttcagcatcg acactccccc gccgaccgtc tcgggttcgc tgcacgtcgg gcacgtgttc
     4021 agctacaccc acaccgacct ggtcgcgcgc taccagcgca tgcagggcaa gcacgtgttc
     4081 tacccgatgg gctgggacga caacggcctg ccgaccgagc ggcgggtgca gaacttctac
     4141 ggggtgcgct gcgacccctc catcgcctat gaccccgact tcaccccgcc cgcgaaaccc
     4201 gaccccaagc gtcaggtgtc gatcagccgc aagaacttca tcgagttgtg ccatgagctc
     4261 accgcggtgg acgagcaggc gttcgagaga ctgtggcgca cgctgggcct gagcgtggac
     4321 tggcggaatc acctctacgc caccatctcg gccgaatcca tgcgcatctc gcagcgggca
     4381 ttcctgcgca actgcgcccg tggcgaggcc tatctggccg aagcgccgac ggtgtgggac
     4441 gtgaccttcc agacggcggt ggcgcaggcc gagcaggagg cccgcgacta ccccggtcac
     4501 taccatcggg tcgcctttca tcgccccg
//
LOCUS       DAOSUG010000226         4511 bp    DNA     linear   ENV 10-JUN-2024
DEFINITION  MAG TPA_asm: Micropruina sp. isolate AMR_MDS_2195
            NODE_8259_length_4511_cov_3.681777, whole genome shotgun sequence.
ACCESSION   DAOSUG010000226 DAOSUG010000000
VERSION     DAOSUG010000226.1
DBLINK      BioProject: PRJNA925477
            BioSample: SAMN32864262
            Sequence Read Archive: SRR4236673
KEYWORDS    WGS; ENV; Metagenome Assembled Genome; MAG; Third Party Data; TPA;
            TPA:assembly.
SOURCE      Micropruina sp. (wastewater metagenome)
  ORGANISM  Micropruina sp.
            Bacteria; Bacillati; Actinomycetota; Actinomycetes;
            Propionibacteriales; Nocardioidaceae; Micropruina.
REFERENCE   1  (bases 1 to 4511)
  AUTHORS   Abdulkadir,N., Saraiva,J.P., Zhang,J., Stolte,S., Gillor,O.,
            Harms,H. and Rocha,U.
  TITLE     Genome-centric analyses of 165 metagenomes show that mobile genetic
            elements are crucial for the transmission of antimicrobial
            resistance genes to pathogens in activated sludge and wastewater
  JOURNAL   Microbiol Spectr 12 (3), e0291823 (2024)
   PUBMED   38289113
REFERENCE   2  (bases 1 to 4511)
  AUTHORS   Abdulkadir,N.
  TITLE     Direct Submission
  JOURNAL   Submitted (24-MAR-2023) Environmental Microbiology, Helmholtz
            Center for Environmental Research -UFZ, Permoserstr 15, Leipzig
            04318, Germany
COMMENT     The annotation was added by the NCBI Prokaryotic Genome Annotation
            Pipeline (PGAP). Information about PGAP can be found here:
            https://www.ncbi.nlm.nih.gov/genome/annotation_prok/
            
            ##Genome-Assembly-Data-START##
            Assembly Method        :: SPAdes v. 3.11.1
            Genome Representation  :: Full
            Expected Final Version :: Yes
            Genome Coverage        :: Not Applicable
            Sequencing Technology  :: Illumina MiSeq
            ##Genome-Assembly-Data-END##
            
            ##Genome-Annotation-Data-START##
            Annotation Provider               :: NCBI
            Annotation Date                   :: 05/04/2023 04:57:10
            Annotation Pipeline               :: NCBI Prokaryotic Genome
                                                 Annotation Pipeline (PGAP)
            Annotation Method                 :: Best-placed reference protein
                                                 set; GeneMarkS-2+
            Annotation Software revision      :: 6.5
            Features Annotated                :: Gene; CDS; rRNA; tRNA; ncRNA
            Genes (total)                     :: 2,776
            CDSs (total)                      :: 2,750
            Genes (coding)                    :: 2,714
            CDSs (with protein)               :: 2,714
            Genes (RNA)                       :: 26
            tRNAs                             :: 24
            ncRNAs                            :: 2
            Pseudo Genes (total)              :: 36
            CDSs (without protein)            :: 36
            Pseudo Genes (ambiguous residues) :: 0 of 36
            Pseudo Genes (frameshifted)       :: 2 of 36
            Pseudo Genes (incomplete)         :: 32 of 36
            Pseudo Genes (internal stop)      :: 2 of 36
            ##Genome-Annotation-Data-END##
FEATURES             Location/Qualifiers
     source          1..4511
                     /organism="Micropruina sp."
                     /mol_type="genomic DNA"
                     /submitter_seqid="NODE_8259_length_4511_cov_3.681777"
                     /isolate="AMR_MDS_2195"
                     /isolation_source="Activated sludge of municipal
                     wastewater treatment plants"
                     /db_xref="taxon:2737536"
                     /environmental_sample
                     /geo_loc_name="Canada"
                     /lat_lon="49.25 N 123.1 W"
                     /collection_date="2015-06-16"
                     /metagenome_source="wastewater metagenome"
                     /note="metagenomic"
     gene            complement(<1..1329)
                     /locus_tag="PLF56_08260"
     CDS             complement(<1..1329)
                     /locus_tag="PLF56_08260"
                     /EC_number="4.1.1.31"
                     /inference="COORDINATES: protein motif:HMM:NF012532.2"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_function: GO:0008964 - phosphoenolpyruvate carboxylase
                     activity [Evidence IEA];
                     GO_process: GO:0006099 - tricarboxylic acid cycle
                     [Evidence IEA];
                     GO_process: GO:0015977 - carbon fixation [Evidence IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="phosphoenolpyruvate carboxylase"
                     /protein_id="HOQ53592.1"
                     /translation="MTDPAAPSGLLDEAGDAQLRRDIRRLGDLLGDTLARQESPGLVD
                     QIEDIRRLSREALAGDRKAQQALTKRISTADLPTAVHLIRAFRTYFHLANIAEQVARI
                     RTLQARQEGTGWLAQAVEKVADELGAPALTTALAKLQIRPVFTAHPTEASRRTTLTQL
                     RQIGDALLATPDVLSPRAERARDRQLTTVIETLWQTDELRLDQPTVEDEARNVMFYAA
                     SLMAGTVPELLDDLADEAALQGAYLPITAAPLALGNWIGGDRDGNPNVTAATTLEVLY
                     RQHLVGIAVLLTALDAVIAEVSTSSRLRKVSPELQQSLESDLADLPELDEHVRRIHVE
                     EPYRLKLHCVRQKLLNTRRRLTEQWPHRPGHDYRGSTALLADLELVRASLIANQAELL
                     ADGHLATLIRTVSAFGLGVATMDVREHAEKHHQALGQLFDRTGELERPYAE"
     gene            complement(1459..2106)
                     /locus_tag="PLF56_08265"
     CDS             complement(1459..2106)
                     /locus_tag="PLF56_08265"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_020388037.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_function: GO:0003677 - DNA binding [Evidence IEA];
                     GO_process: GO:0000160 - phosphorelay signal transduction
                     system [Evidence IEA];
                     GO_process: GO:0006355 - regulation of DNA-templated
                     transcription [Evidence IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="response regulator transcription factor"
                     /protein_id="HOQ53593.1"
                     /translation="MITVAIVEDDPLIRAGIAALVGAQPDLSVAGQAADGDEAVALVT
                     ATAPDVLLMDVRMPRLGGIDATRAVCAGELAPPVLILTTFDGDPDVLQALRAGASGYL
                     LKRNPDDLPAAIRRVHEGEVWLDPAVAGHVLAALAALPQPGVPSSRVEVLTAREREVL
                     ALLAEGLSNAELAQRLFVGEGTIKTHMSRILFKTGCRDRAQATALAFTSGLVRVG"
     gene            complement(2117..3256)
                     /locus_tag="PLF56_08270"
     CDS             complement(2117..3256)
                     /locus_tag="PLF56_08270"
                     /inference="COORDINATES: protein motif:HMM:NF019350.2"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_component: GO:0016020 - membrane [Evidence IEA];
                     GO_function: GO:0000155 - phosphorelay sensor kinase
                     activity [Evidence IEA];
                     GO_function: GO:0046983 - protein dimerization activity
                     [Evidence IEA];
                     GO_process: GO:0000160 - phosphorelay signal transduction
                     system [Evidence IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="histidine kinase"
                     /protein_id="HOQ53594.1"
                     /translation="MAVHVPQELMNANGRNLVVVGGAIAVDLLFWDGEVRLAGGGELP
                     MWLPVVTTVVAHACLLDRRTRPVAVFVVQAAFAMVSVVVPLWQPVAGLLVATFAIAAN
                     TGSRVARWGWLAAVPLIAHAVTQSLWFNSKWVGLLQAGTLYLVAGAACWVAGRRVRAR
                     GGQLRAWHAEQERLHTEATLDERLALARELHDGVANTITAVLVQAAAARASSCGDPDA
                     LHRIEATARRAMHEIQTTLRLMPHAAGRPSGPSLDDLPNLLDLGRAAGLDVVHTSSGT
                     PRNLDPAASAAAYRAVQEGITNTLKYAPAGTRCVVALEWRPDELVIGIVDHPRSGRAC
                     ADLPATNGRGLAGLADRLAGLGGAVESGAHESGFRLEARLPVGVR"
     gene            complement(3229..3693)
                     /locus_tag="PLF56_08275"
     CDS             complement(3229..3693)
                     /locus_tag="PLF56_08275"
                     /inference="COORDINATES: ab initio
                     prediction:GeneMarkS-2+"
                     /note="Derived by automated computational analysis using
                     gene prediction method: GeneMarkS-2+."
                     /codon_start=1
                     /transl_table=11
                     /product="hypothetical protein"
                     /protein_id="HOQ53595.1"
                     /translation="MAKQTKKFQLMIKDLEAQVGPVITGHPHPQATGPVFIAVFGTRP
                     DASHAEVLGSGNLIKNDLVLGDVATVTALRAGARPTMVGIAWVDATGNHTQARSVANF
                     GHRDGEASSSQDMWAMELSSPSTAPDPSIPPGASQASGWCFLFPWLSMCHKN"
     gene            complement(4296..>4511)
                     /locus_tag="PLF56_08280"
     CDS             complement(4296..>4511)
                     /locus_tag="PLF56_08280"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_013865523.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_function: GO:0008184 - glycogen phosphorylase activity
                     [Evidence IEA];
                     GO_process: GO:0005975 - carbohydrate metabolic process
                     [Evidence IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="glycogen/starch/alpha-glucan phosphorylase"
                     /protein_id="HOQ53596.1"
                     /translation="QPASEEDSTSAMALADFGGYIEAQERVDAYYADTEAWTRSAILN
                     VSRSGFFSSDRSMKDYIKRIWHTNPVL"
BASE COUNT          633 a         1556 c         1648 g          674 t
ORIGIN      
        1 ctcggcgtag ggccgctcga gttcgccggt gcggtcgaac aactgcccaa gggcctggtg
       61 gtgcttctcg gcgtgctcgc gcacgtccat ggtggccacg ccgagcccga aggcgctcac
      121 cgtgcggatc agggtggcga ggtgcccatc ggccagcagt tcggcctggt tggcgatcag
      181 ggacgcacgc accaactcga ggtcggccag tagtgcggta ctgccccgat aatcgtggcc
      241 cggccggtgc ggccactgct cggtcaaccg gcgccgcgtg ttgagcagct tctgtcgcac
      301 gcagtgcagc ttgagtcggt acggctcttc gacgtggatg cggcggacgt gctcgtccag
      361 ctcgggcagg tcggccaggt cgctctcgag gctctgctgc aactcggggc tgaccttgcg
      421 caaccgactc gacgtcgaca cttcggcgat caccgcatcg agcgcggtga gcagcacggc
      481 gatgccgacc aggtgctgcc ggtacagcac ttcgagcgtg gtggcggcgg tcacgttggg
      541 gttgccgtcg cggtcgccgc cgatccagtt gccgagggcc agcggggcgg ccgtgatggg
      601 caggtaggca ccctgaaggg ccgcctcgtc ggcgagatcg tccagcagct cgggcacggt
      661 gcccgccatc aggctggccg cgtagaacat cacgttgcgc gcctcgtcct cgacggtggg
      721 ctggtcgagc cgcaactcgt cggtctgcca cagcgtctcg atcaccgtgg tgagctgccg
      781 gtcgcgcgcc cgctcggctc gcgggctcag cacgtcgggg gtggcgagca gcgcgtcgcc
      841 gatctggcgc agttgggtca gggtggtgcg gcggctcgcc tcggtggggt gtgcggtgaa
      901 caccggacgg atctgcagct tcgccaacgc cgtggtcagg gccggggcgc cgagctcgtc
      961 cgccaccttc tcaacggcct gcgccaacca gccggtgccc tcttgacggg cctgcagggt
     1021 gcggatgcgt gccacctgct cggcgatgtt ggccaggtgg aagtaggtgc gaaaggcccg
     1081 gatcaggtgg acggcggtgg gcaggtcggc ggtgctgatg cgcttggtca gcgcctgttg
     1141 cgccttgcgg tcaccggcca gagcctcgcg cgagagccgg cgaatgtctt cgatctggtc
     1201 gaccaggccg ggcgactcct ggcgggccag ggtgtcgccc agtaggtcgc cgagccggcg
     1261 gatgtcgcgt cggagttggg cgtcgccggc ttcgtcgaga aggccggacg gggctgcggg
     1321 atcggtcacg ggcgggaccc tcctggtgcg ctcgacagcg gaggacggca ctgtccggag
     1381 tcagcagggt agtggtgcgc ggaacctccg gcgccggtcg tctcggcacg cgagtgccgg
     1441 cgaagcgtcc tggccggctc agccgacgcg caccagcccc gaggtgaacg cgagcgcggt
     1501 agcctgcgcg cggtcgcggc agccggtctt gaacaggatc cgcgacatgt gggtcttgat
     1561 cgtgccctcg ccgacgaaca ggcgctgcgc caactcggcg ttgctcagac cctccgcgag
     1621 cagcgccagc acctcacgtt cacgggcggt cagcacctcg acccgactgc tcggcacccc
     1681 gggctgcggc aatgccgcga gcgccgccag cacgtggccg gcgaccgccg ggtcgagcca
     1741 cacctcaccc tcgtgcaccc ggcggatcgc ggccggcagg tcgtccgggt tgcgcttcag
     1801 cagatagccg gacgccccgg cgcgcaacgc ctgcagcacg tcggggtcgc cgtcgaacgt
     1861 ggtcaggatc agcaccggcg gcgccaactc gccggcgcag acggcgcggg tggcgtcgat
     1921 gccgccgagc cggggcatgc gcacgtccat cagcagcacg tcgggggcgg tcgcggtgac
     1981 cagggcgacg gcctcgtcgc cgtccgcggc ctggccagcc acggacaggt ccggttgtgc
     2041 accgaccaga gcggcgatgc ccgcccggat cagcgggtcg tcctcgacga tcgcaacggt
     2101 gatcatcgcg gtgccctcac cgaacgccca ccggcagccg cgcctccagc cggaatcccg
     2161 actcgtgcgc accagactcg acggccccgc ccaggcccgc gagccggtcg gcgaggccgg
     2221 ccaggccccg tccgttggtg gcgggcaggt cggcgcacgc ccgtccggac cgggggtgat
     2281 cgacgatgcc gatcaccagt tcgtccggac gccactcgag ggcgaccacg caacgggtgc
     2341 cggcgggtgc gtacttgagc gtgttggtga tgccctcctg cacggcccgg taggccgccg
     2401 cggacgccgc cggatcgagg ttgcgcgggg taccggacga ggtgtgcacg acgtccaggc
     2461 ccgcggcccg gcccaggtcg agcaggttcg gcaggtcgtc cagcgagggt ccggacggac
     2521 gcccggcggc gtgcggcatc agccgcagcg tggtctggat ctcgtgcatg gcccgccggg
     2581 cggtcgcttc gatgcggtgc agcgcgtcgg gatcgccgca cgaggacgcc cgcgccgccg
     2641 cggcctgtac caggacggcg gtgatggtgt tggccacgcc gtcgtgcaat tcgcgggcca
     2701 gcgcgaggcg ttcgtcgagg gtggcctcgg tgtgcagccg ctcctgttcg gcgtgccagg
     2761 cgcgcagttg gccgccgcgg gctcgcaccc gccgtccggc gacccagcag gcggcaccgg
     2821 cgaccaggta cagcgtgcct gcctgcaaga gcccgaccca tttgctgttg aaccagagcg
     2881 actgcgtcac cgcgtgcgcg atcaatggca ccgcagccag ccaaccccag cgcgccaccc
     2941 gtgaaccggt gttcgcggcg atcgcgaagg tggccaccag cagcccggct accggttgcc
     3001 acagcggcac caccacgctg accatcgcga acgcggcctg cacgacgaac acggcgaccg
     3061 gacgcgtccg gcgatcgagc aggcaggcat gcgcgacgac ggtggtcacc accggcagcc
     3121 acatcggcag ttcgccaccc ccggccagcc gcacctcgcc gtcccagaac agcaggtcga
     3181 cggcgatcgc gccgccgacc accacgaggt tgcggccgtt ggcgttcatc agttcttgtg
     3241 gcacatggac agccacggaa acaggaagca ccagccgctg gcctgtgacg ctcccggggg
     3301 aatggacgga tccggcgccg tgctgggcga gctcaactcc atcgcccaca tgtcctggct
     3361 ggagctggcc tcgccgtcgc ggtgaccgaa gttcgccacg ctgcgcgcct gggtgtggtt
     3421 gcccgtggcg tccacccagg cgatgcccac catggtcgga cgggcaccgg cgcgcagcgc
     3481 tgtcacggtg gctacgtcgc cgagcacgag gtcgttcttg atcaggttgc cgctgccgag
     3541 cacctcggcg tgggaggcgt cgggtcgggt accgaacacc gcgatgaaca ccgggccggt
     3601 ggcctggggg tgcgggtggc cggtgatcac gggaccgacc tgcgcctcca gatccttgat
     3661 catcagttgg aacttcttgg tctgcttggc catgggggct ccttcgccgg cgagtgcccc
     3721 tcatgagtac gcctcgcgcc gcggtgatac cgcgctgccg aacggggcgc ccacatgtcg
     3781 tgacccgtcg tcccggcgca ggacgggatc ctggcccgtc gtcccggcgt cggccgggat
     3841 ctcctccgta cggcgagatc ccggggcaag cccgggatga cgaataggcc gggatgacga
     3901 ccccgttatc ccggcgcggg ccgggatctt gtctatacgg cgagatcccg gggcaagccc
     3961 gggatgacga ataggccgag atcatgcacc tcgtcatccc ggcgaatgcc gggatcctga
     4021 cccccgtcat cccggccatt gccgggatct cctccgtgcg gcgagatccc ggggcaagcc
     4081 cgggatgacg aataggccgg gatcctgacc ctcgtcatcc cggcgcaggc cgggatcttg
     4141 tctgcacgtc gagatcccgg ggcaagcccg ggatgacgag tgaggccggg atcctgatcc
     4201 ccgtcatccc ggcgaatgcc gggatctcgt ctgtgtgttg agatcccggc gcaggcccgg
     4261 gatgacgagt gcgccgcggt cgagagcccc gtggctcaga gcaccgggtt ggtgtgccag
     4321 atccgcttga tgtagtcctt catcgaccgg tcggagctga agaagccgct gcgggacacg
     4381 ttcaggatcg ccgagcgcgt ccaggcctcg gtgtcggcgt agtaggcgtc cactcgctcc
     4441 tgcgcctcga tgtacccgcc gaagtcggcc agcgccatgg cgctggtgct gtcctcttcg
     4501 gaggccggct g
//
LOCUS       DAOSUG010000227         4488 bp    DNA     linear   ENV 10-JUN-2024
DEFINITION  MAG TPA_asm: Micropruina sp. isolate AMR_MDS_2195
            NODE_8319_length_4488_cov_3.919016, whole genome shotgun sequence.
ACCESSION   DAOSUG010000227 DAOSUG010000000
VERSION     DAOSUG010000227.1
DBLINK      BioProject: PRJNA925477
            BioSample: SAMN32864262
            Sequence Read Archive: SRR4236673
KEYWORDS    WGS; ENV; Metagenome Assembled Genome; MAG; Third Party Data; TPA;
            TPA:assembly.
SOURCE      Micropruina sp. (wastewater metagenome)
  ORGANISM  Micropruina sp.
            Bacteria; Bacillati; Actinomycetota; Actinomycetes;
            Propionibacteriales; Nocardioidaceae; Micropruina.
REFERENCE   1  (bases 1 to 4488)
  AUTHORS   Abdulkadir,N., Saraiva,J.P., Zhang,J., Stolte,S., Gillor,O.,
            Harms,H. and Rocha,U.
  TITLE     Genome-centric analyses of 165 metagenomes show that mobile genetic
            elements are crucial for the transmission of antimicrobial
            resistance genes to pathogens in activated sludge and wastewater
  JOURNAL   Microbiol Spectr 12 (3), e0291823 (2024)
   PUBMED   38289113
REFERENCE   2  (bases 1 to 4488)
  AUTHORS   Abdulkadir,N.
  TITLE     Direct Submission
  JOURNAL   Submitted (24-MAR-2023) Environmental Microbiology, Helmholtz
            Center for Environmental Research -UFZ, Permoserstr 15, Leipzig
            04318, Germany
COMMENT     The annotation was added by the NCBI Prokaryotic Genome Annotation
            Pipeline (PGAP). Information about PGAP can be found here:
            https://www.ncbi.nlm.nih.gov/genome/annotation_prok/
            
            ##Genome-Assembly-Data-START##
            Assembly Method        :: SPAdes v. 3.11.1
            Genome Representation  :: Full
            Expected Final Version :: Yes
            Genome Coverage        :: Not Applicable
            Sequencing Technology  :: Illumina MiSeq
            ##Genome-Assembly-Data-END##
            
            ##Genome-Annotation-Data-START##
            Annotation Provider               :: NCBI
            Annotation Date                   :: 05/04/2023 04:57:10
            Annotation Pipeline               :: NCBI Prokaryotic Genome
                                                 Annotation Pipeline (PGAP)
            Annotation Method                 :: Best-placed reference protein
                                                 set; GeneMarkS-2+
            Annotation Software revision      :: 6.5
            Features Annotated                :: Gene; CDS; rRNA; tRNA; ncRNA
            Genes (total)                     :: 2,776
            CDSs (total)                      :: 2,750
            Genes (coding)                    :: 2,714
            CDSs (with protein)               :: 2,714
            Genes (RNA)                       :: 26
            tRNAs                             :: 24
            ncRNAs                            :: 2
            Pseudo Genes (total)              :: 36
            CDSs (without protein)            :: 36
            Pseudo Genes (ambiguous residues) :: 0 of 36
            Pseudo Genes (frameshifted)       :: 2 of 36
            Pseudo Genes (incomplete)         :: 32 of 36
            Pseudo Genes (internal stop)      :: 2 of 36
            ##Genome-Annotation-Data-END##
FEATURES             Location/Qualifiers
     source          1..4488
                     /organism="Micropruina sp."
                     /mol_type="genomic DNA"
                     /submitter_seqid="NODE_8319_length_4488_cov_3.919016"
                     /isolate="AMR_MDS_2195"
                     /isolation_source="Activated sludge of municipal
                     wastewater treatment plants"
                     /db_xref="taxon:2737536"
                     /environmental_sample
                     /geo_loc_name="Canada"
                     /lat_lon="49.25 N 123.1 W"
                     /collection_date="2015-06-16"
                     /metagenome_source="wastewater metagenome"
                     /note="metagenomic"
     gene            <1..1048
                     /locus_tag="PLF56_08285"
     CDS             <1..1048
                     /locus_tag="PLF56_08285"
                     /inference="COORDINATES: protein motif:HMM:NF012892.2"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_component: GO:0016020 - membrane [Evidence IEA];
                     GO_function: GO:0005215 - transporter activity [Evidence
                     IEA];
                     GO_function: GO:0005524 - ATP binding [Evidence IEA];
                     GO_function: GO:0016887 - ATP hydrolysis activity
                     [Evidence IEA]"
                     /codon_start=2
                     /transl_table=11
                     /product="HAD-IC family P-type ATPase"
                     /protein_id="HOQ53597.1"
                     /translation="TAGIKVTMITGDHAGTATAISREMGIFDESTEAAVTGAELEAAS
                     DSELRSIVNRTETFARTSPEHKLRLVKALQANGEVVAMTGDGVNDAPALKRADVGVAM
                     GIKGTEATKEAADIVLADDNFATIEHAVEEGRRINDNLNKSVLFLLPTNGAQSLVVLV
                     AVVFGLTMPLAPVQILWVNMITAVTLSLALAYEPAEADIMRRPPRLPGTPLVGVRYLP
                     RLALASALIAAATLATFVIASRLGAPLPVAQTWAVNTLALGQAAYLFNSRFLRESSMR
                     AEAMGGNPVAWAVVGVMLVLQIAFVYLPVLNTWFGSAAVDALGWLVPLGFAVLIFVLM
                     EGVKALVRKGEMTR"
     gene            1229..2290
                     /locus_tag="PLF56_08290"
     CDS             1229..2290
                     /locus_tag="PLF56_08290"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_020391889.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_function: GO:0000976 - transcription cis-regulatory
                     region binding [Evidence IEA];
                     GO_function: GO:0003700 - DNA-binding transcription factor
                     activity [Evidence IEA];
                     GO_function: GO:0043565 - sequence-specific DNA binding
                     [Evidence IEA];
                     GO_process: GO:0006351 - DNA-templated transcription
                     [Evidence IEA];
                     GO_process: GO:0006355 - regulation of DNA-templated
                     transcription [Evidence IEA];
                     GO_process: GO:0045892 - negative regulation of
                     DNA-templated transcription [Evidence IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="LacI family DNA-binding transcriptional
                     regulator"
                     /protein_id="HOQ53598.1"
                     /translation="MGSHAPVTMKTLAQRLGISVTTVSNAYSKPDRLSEVLRQQIFAQ
                     AKELGYCGPSAAGRALRSGKSGICGFLFGAHLSAAFSDPYTVMFLAGLGEALERYDTS
                     VLLLRSDGGDDDEALQRAPVDAVVSGSPASRHPGLQRLAGRGVRVVSTMQSDEPEWVG
                     IDDRLGGRLVGRLLARLGHRRVTVLVPGSLSPAGDPIQVELASGGVLPGDFPGLDCGF
                     AEKRIAGLRETLPDASIRVVSAGDNVKESGRHAGGYVLDDQRRPSAVVAVSDVLALGV
                     WDAALQRGLTPGRDVSIAGFDDLPDAAFVGLTSVHQPIAEKGRLAGRLAMDPDYPQRQ
                     VLLPLELKVRASTGPAPVG"
     gene            2356..2565
                     /locus_tag="PLF56_08295"
     CDS             2356..2565
                     /locus_tag="PLF56_08295"
                     /inference="COORDINATES: ab initio
                     prediction:GeneMarkS-2+"
                     /note="Derived by automated computational analysis using
                     gene prediction method: GeneMarkS-2+."
                     /codon_start=1
                     /transl_table=11
                     /product="hypothetical protein"
                     /protein_id="HOQ53599.1"
                     /translation="MTSTHFVTMIQSAEHGASLVAPIHDRRFSPRHEHTRALGARWLR
                     AHAALSLRWAQRIDPQASEQHLAAA"
     gene            complement(2896..>4488)
                     /locus_tag="PLF56_08300"
     CDS             complement(2896..>4488)
                     /locus_tag="PLF56_08300"
                     /inference="COORDINATES: ab initio
                     prediction:GeneMarkS-2+"
                     /note="Derived by automated computational analysis using
                     gene prediction method: GeneMarkS-2+."
                     /codon_start=1
                     /transl_table=11
                     /product="thioredoxin domain-containing protein"
                     /protein_id="HOQ53600.1"
                     /translation="RRDEVLTSADSIVAHLSDEFGTLPASGDLDLAQLHEKVSADFDP
                     VHGGFGSAPKFPAPGLIDALLVTGEPASLDLAQRSLEAMLRGGIHDQVGGGFHRYSVD
                     AGWVVPHFEKMLYDNALLLGSYTRAWCRTADHDHGLRLLFERAVRGIVRCLADDLQLD
                     EGGFAASLDADSADIGGTVHEGIFYVWSPELLADALGQPDADWAGEVFHVTAAGTFEH
                     GLSTLQLRGTPDWSRLDRVIERLGEVRSTRFVPARDDKLVAAWNGFAIDSLVNAAMVF
                     GEPDWLELAQRAAEATWGLWVDGALHRVGIDGVAGDAPGMAEDHGALALGLARLAGAT
                     GDAVWLDRAITLLEAALPAFDAGEGGFYDAPVTDGLRPFARARDTSDNPTPSGTACLV
                     AALRLVALLADRPDFAERADAAARTLHGMVEAAPRFAGWALADALIEDQARSRLTPAV
                     AVIVTDEASGSIGELARAAWRMAPAGTAIVVGPAGTQGFAHHFDDRTAQDGPTAYVCR
                     GTVCFAPASAPKQLRAALWQRP"
BASE COUNT          698 a         1591 c         1544 g          655 t
ORIGIN      
        1 caccgctggc atcaaggtga ccatgatcac cggcgaccac gcgggcaccg caaccgcgat
       61 cagtcgcgag atgggcatct tcgacgagtc gaccgaggcg gccgtcaccg gcgccgaact
      121 cgaggccgcc tcggacagtg aactgcgcag catcgtcaac cgcaccgaga ccttcgcccg
      181 caccagcccc gagcacaagc tgcggctggt caaggcgctg caggccaacg gtgaagtcgt
      241 ggccatgacg ggcgacgggg tcaacgacgc ccctgcgctc aagcgtgcgg acgtgggggt
      301 ggccatgggc atcaagggca ccgaggccac caaggaggcg gccgacatcg tgctcgccga
      361 cgacaacttc gccaccatcg agcacgcggt cgaagagggc cgccgcatca acgacaacct
      421 gaacaagtcg gtgctgtttc tgctgccgac caacggagcc cagtcgctgg tggtgctggt
      481 ggcggtggtg ttcgggctga ccatgcccct cgccccggtg cagattctgt gggtcaacat
      541 gatcacggcg gtcaccttgt cgttggccct ggcctacgaa cccgccgaag ccgacatcat
      601 gcggcgcccg ccgcggctgc ccggcacgcc gttggtcggc gtacggtacc tgccgcggct
      661 ggcgctggcc tcggcgctga tcgccgccgc gacgctggcc acgttcgtga tcgcgtccag
      721 gctcggtgcg ccgctgccgg tggcgcagac ctgggcggtc aacaccctgg cgcttggcca
      781 ggcggcctac ctgttcaact cgcggttcct gcgcgaatcg agcatgcggg cggaggcaat
      841 gggcggcaac ccggtggcct gggcggtggt cggcgtcatg ctggtgctgc agatcgcctt
      901 cgtctacctg ccggtgctga acacctggtt cggttcggcg gccgtggatg ctctggggtg
      961 gctcgtgccg ctcggcttcg cggtgctcat cttcgtgctg atggagggcg tcaaggcgct
     1021 ggtgcgcaag ggcgaaatga cccgctgagc ctgatcgctg gtcgggcctg tcgaaaccct
     1081 cgcgagccgg gtctcgactc ggtcaaccaa ccggatcagt gcgttgatca ccgttcactt
     1141 actgacaccc attgccgggt tgatcgataa agtgtaacgt tcactttatc gatcaacgcc
     1201 tcgtcgacgt acccggaagg agccagtcat gggtagccac gccccggtca ccatgaagac
     1261 cctggcccag cggctgggta tctcggtgac caccgtgtcg aacgcctaca gcaagccgga
     1321 tcggctgtcg gaggtgttgc ggcagcagat cttcgcccag gcgaaagagt tgggttactg
     1381 cggtccgagc gccgcgggcc gcgctctgcg ctcgggcaag agcggcatct gcggcttttt
     1441 gttcggcgcg cacctgtcgg ccgcgttctc ggacccgtac acggtgatgt ttctggccgg
     1501 cctcggcgaa gcactggaac ggtacgacac ctcggtcctg ctgctgcggt ccgatggtgg
     1561 cgacgacgac gaggcactgc aacgggcacc cgtcgacgcg gtggtcagcg gctccccggc
     1621 gagccgccac ccgggcctgc agcggctggc gggccgcggg gtccgggtgg tcagcaccat
     1681 gcagtccgac gagcccgaat gggtcggcat cgacgaccgg ttgggcggac ggctggtggg
     1741 tcggctactg gctcgcctgg gtcaccgcag ggtcaccgtg ctcgttccgg gctcgctgtc
     1801 gccggcgggt gaccccatcc aggtcgaact ggcctcgggc ggagtgctgc cgggtgactt
     1861 tccgggcctg gactgtggct tcgccgagaa acggatcgcc ggactgcgcg agacacttcc
     1921 cgacgccagc atccgggtgg tgagtgcggg cgacaacgtc aaagagtccg gacgccacgc
     1981 aggggggtac gtgctcgacg accaacgccg tccgtccgcg gtcgttgcgg tcagcgacgt
     2041 actggccctg ggggtgtggg acgcggcgct gcagcggggg ttgacgcccg gtcgggacgt
     2101 ctcgatcgcc ggcttcgacg atctgccaga tgcggcgttc gtgggactca cctcggtgca
     2161 ccagccgatc gccgagaagg gacgcctcgc cggccgcctc gccatggacc ccgactaccc
     2221 acaacggcag gttctgctgc ccctcgaact gaaggtgcgc gccagcaccg gccccgcacc
     2281 tgtcggctga cggtctgaac cacaggccgc ccgaaccgtt acgacccgct caccaagcgc
     2341 tgaaagacac caaccatgac ctccacccat ttcgtcacga tgatccagtc cgccgagcac
     2401 ggcgcctccc tggtcgcccc gatccacgac cgccgcttca gcccccgaca cgagcacacc
     2461 cgcgcgctgg gcgctcgctg gctgcgggcg cacgcggcac tgtcccttcg atgggcccag
     2521 cggatcgacc cgcaggcgtc cgagcagcac ctggccgccg cctgagtcca agcgagccgg
     2581 catgccagtc cgcacggtgt cggcaaccgc cggtcgagcc aagcgtgggc agcggcttgg
     2641 ccatgctcaa ccggcgaatc gtgcgtcgat cagctcaact gccccagccc gccggacccg
     2701 caccacggca cgcctgaaaa ccctgcacca gtgcgaccgt ccccgcctca gcacgctggg
     2761 aggcttgcca ctggtgcagg gattgcaggc gaaggaccga gtacgtgccc cacagcacta
     2821 ggccatgatc acgaacgacg gtcatgatcg ccccggtctg ggtggatgga tcacgccagt
     2881 gcgcgagccg tcgggtcagg gccgctgcca cagggctgcg cgtaactgct tcggcgcgct
     2941 cgcgggagcg aaacacacgg tgcctctgca cacgtaggcg gtcggcccgt cctgggcagt
     3001 ccggtcgtcg aagtggtggg cgaaaccctg cgtgcccgcg ggcccgacca cgatggcggt
     3061 accggcgggc gccatccgcc aggctgcccg cgcgagttca ccgatcgatc cggacgcctc
     3121 gtccgtcacg atcaccgcga ccgccggggt gagccggctg cgcgcctgat cctcgatcag
     3181 cgcatcggcc agcgcccagc cggcgaaccg cggcgccgcc tcgaccatgc cgtgcagggt
     3241 gcgcgcggcg gcgtccgccc gttcggcgaa gtcgggccgg tcggccagca acgccaccag
     3301 ccgcagcgcc gccaccaggc acgcggtgcc ggacggcgtc gggttgtcgg aggtgtcgcg
     3361 cgccctcgcg aacggacgca ggccgtcggt caccggcgcg tcgtagaagc cgccctcgcc
     3421 ggcatcgaag gccggcagcg cggcctcgag cagcgtgatg gcccgatcga gccagaccgc
     3481 atcgccggtg gcgccggcca accgggccag ccccaacgcc agcgctccgt gatcctcggc
     3541 catgccgggc gcatcgcccg ccacgccgtc gatgccgacc cggtgcagcg cgccgtccac
     3601 ccacagcccc caggtcgcct cggcggcccg ctgcgccagc tccagccagt ccggctcgcc
     3661 gaacaccatc gcggcgttca ccagagaatc gatggcgaag ccgttccacg cggcgaccag
     3721 cttgtcgtcc cgagcgggga cgaaccgggt cgagcgcacc tcgcccaacc gctcgatcac
     3781 tcggtccagc cgcgaccagt ccggcgttcc acgcaactgc aacgtggaga ggccgtgctc
     3841 gaaggtgccc gcggcggtga cgtggaacac ctcaccggcc cagtcggcgt ccggctgacc
     3901 cagggcgtcg gcgagcagtt cgggcgacca gacgtagaag atgccttcgt gcacggtgcc
     3961 gccgatgtcg gccgagtcgg cgtccaggct ggctgcgaag ccgccctcgt ccagctgcag
     4021 gtcatcggcc agacaccgga cgatgccgcg cacggcccgc tcgaacagca gccgcaggcc
     4081 gtggtcgtga tcggcggtgc ggcaccaggc gcgggtgtag ctgccgagca gcagggcgtt
     4141 gtcgtagagc atcttctcga agtgcggcac cacccagccg gcgtcgacgc tgtagcggtg
     4201 gaagccaccg cccacctgat cgtggatgcc gccgcgcaac atcgcctcca ggctgcgctg
     4261 ggcgagatcg agcgaggccg gctcaccggt gaccagcagg gcgtcgatca gtccgggcgc
     4321 cgggaatttg ggtgcgctgc cgaagccgcc gtgcaccggg tcgaaatcgg ccgagacctt
     4381 ctcgtggagc tgcgccagat cgagatcacc ggacgccggc aaggtgccga actcgtcgct
     4441 gaggtgggcg acaatcgagt ccgcactggt cagcacctcg tcgcgccg
//
LOCUS       DAOSUG010000228         4475 bp    DNA     linear   ENV 10-JUN-2024
DEFINITION  MAG TPA_asm: Micropruina sp. isolate AMR_MDS_2195
            NODE_8342_length_4475_cov_3.604299, whole genome shotgun sequence.
ACCESSION   DAOSUG010000228 DAOSUG010000000
VERSION     DAOSUG010000228.1
DBLINK      BioProject: PRJNA925477
            BioSample: SAMN32864262
            Sequence Read Archive: SRR4236673
KEYWORDS    WGS; ENV; Metagenome Assembled Genome; MAG; Third Party Data; TPA;
            TPA:assembly.
SOURCE      Micropruina sp. (wastewater metagenome)
  ORGANISM  Micropruina sp.
            Bacteria; Bacillati; Actinomycetota; Actinomycetes;
            Propionibacteriales; Nocardioidaceae; Micropruina.
REFERENCE   1  (bases 1 to 4475)
  AUTHORS   Abdulkadir,N., Saraiva,J.P., Zhang,J., Stolte,S., Gillor,O.,
            Harms,H. and Rocha,U.
  TITLE     Genome-centric analyses of 165 metagenomes show that mobile genetic
            elements are crucial for the transmission of antimicrobial
            resistance genes to pathogens in activated sludge and wastewater
  JOURNAL   Microbiol Spectr 12 (3), e0291823 (2024)
   PUBMED   38289113
REFERENCE   2  (bases 1 to 4475)
  AUTHORS   Abdulkadir,N.
  TITLE     Direct Submission
  JOURNAL   Submitted (24-MAR-2023) Environmental Microbiology, Helmholtz
            Center for Environmental Research -UFZ, Permoserstr 15, Leipzig
            04318, Germany
COMMENT     The annotation was added by the NCBI Prokaryotic Genome Annotation
            Pipeline (PGAP). Information about PGAP can be found here:
            https://www.ncbi.nlm.nih.gov/genome/annotation_prok/
            
            ##Genome-Assembly-Data-START##
            Assembly Method        :: SPAdes v. 3.11.1
            Genome Representation  :: Full
            Expected Final Version :: Yes
            Genome Coverage        :: Not Applicable
            Sequencing Technology  :: Illumina MiSeq
            ##Genome-Assembly-Data-END##
            
            ##Genome-Annotation-Data-START##
            Annotation Provider               :: NCBI
            Annotation Date                   :: 05/04/2023 04:57:10
            Annotation Pipeline               :: NCBI Prokaryotic Genome
                                                 Annotation Pipeline (PGAP)
            Annotation Method                 :: Best-placed reference protein
                                                 set; GeneMarkS-2+
            Annotation Software revision      :: 6.5
            Features Annotated                :: Gene; CDS; rRNA; tRNA; ncRNA
            Genes (total)                     :: 2,776
            CDSs (total)                      :: 2,750
            Genes (coding)                    :: 2,714
            CDSs (with protein)               :: 2,714
            Genes (RNA)                       :: 26
            tRNAs                             :: 24
            ncRNAs                            :: 2
            Pseudo Genes (total)              :: 36
            CDSs (without protein)            :: 36
            Pseudo Genes (ambiguous residues) :: 0 of 36
            Pseudo Genes (frameshifted)       :: 2 of 36
            Pseudo Genes (incomplete)         :: 32 of 36
            Pseudo Genes (internal stop)      :: 2 of 36
            ##Genome-Annotation-Data-END##
FEATURES             Location/Qualifiers
     source          1..4475
                     /organism="Micropruina sp."
                     /mol_type="genomic DNA"
                     /submitter_seqid="NODE_8342_length_4475_cov_3.604299"
                     /isolate="AMR_MDS_2195"
                     /isolation_source="Activated sludge of municipal
                     wastewater treatment plants"
                     /db_xref="taxon:2737536"
                     /environmental_sample
                     /geo_loc_name="Canada"
                     /lat_lon="49.25 N 123.1 W"
                     /collection_date="2015-06-16"
                     /metagenome_source="wastewater metagenome"
                     /note="metagenomic"
     gene            complement(85..297)
                     /locus_tag="PLF56_08305"
     CDS             complement(85..297)
                     /locus_tag="PLF56_08305"
                     /inference="COORDINATES: ab initio
                     prediction:GeneMarkS-2+"
                     /note="Derived by automated computational analysis using
                     gene prediction method: GeneMarkS-2+."
                     /codon_start=1
                     /transl_table=11
                     /product="hypothetical protein"
                     /protein_id="HOQ53601.1"
                     /translation="MVAAFVDDPAKGLDSDASFSKGDALSAMVLDYEGGIVTGRRPVS
                     DLAELRDRWRKGGGDQMRSELEEQLR"
     gene            complement(457..1614)
                     /locus_tag="PLF56_08310"
     CDS             complement(457..1614)
                     /locus_tag="PLF56_08310"
                     /inference="COORDINATES: protein motif:HMM:NF024799.2"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="substrate-binding domain-containing protein"
                     /protein_id="HOQ53602.1"
                     /translation="MSRLLSKPLAVGCALTVGAALALAGCQRGSDAASGPTSAAATAA
                     GACTDVIASSKAAVDKASAADAKWDGPTTGPKAASGKTIVYVAQSMQNPGVAGVETGL
                     KDAAAAIGWTLKTIDGQGTPAGIQNAFNQALTLKPDGIIIGGFDPNSTADQVKQANAA
                     NIPLVGWQSLATPGPSDSPKLFSNVTTKVEDVAKISADYVIAKSNGTAGVVIFTDSSI
                     PFAEGKSQMIQKQLQTCSSVNVLKYSNIPLADVASRMPTETSALLSSLGDKWTYSVAI
                     NDVYFENATAAFRQAGKTNETAPQNVGAGDGDAAALTRIRTGDFQAATVPSPLRSEGW
                     QIVDELNRAFAGEKASGYVPQIHVSTKENATADNFWDPVGYQDAYKAIWGK"
     gene            complement(1647..2813)
                     /locus_tag="PLF56_08315"
     CDS             complement(1647..2813)
                     /locus_tag="PLF56_08315"
                     /inference="COORDINATES: protein motif:HMM:NF014684.2"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_function: GO:0042626 - ATPase-coupled transmembrane
                     transporter activity [Evidence IEA];
                     GO_function: GO:0140359 - ABC-type transporter activity
                     [Evidence IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="ABC transporter permease"
                     /protein_id="HOQ53603.1"
                     /translation="MTTATAAPTRPARRRRGGGSFVGTYGLLVLAVLLFAVFAILMPG
                     TFLTQNNLNAILGANSIPAILALGAMIPIATGKFDVSIGYALGMAHVLMMVMVVNNIN
                     WMLAAVIVLVLLTCVGIVNGLLVELGKIDSFVATLGTGSVLYAITGAVTNGSRVVPTG
                     EGLPAGFTDLYDSKIAGIPIAAWYVTVIAVVLWIVLERLPLGRYFYVLGSNQRAADLI
                     GLPTRRYSVYAFAACSFLVGVAGILLASQQHVGNPSVGSEYMLPAFVAALLGSTVIRP
                     GRPNAAGTIVAVVTLAIGLAGIGQLGANFWVNPLFNGVTLLIAVGLAGWSARRKIMAG
                     IAANKAVDSGAPASPGGTGAHAAEAELVDTVGPSTSASAVIDPSADPSGPSSGH"
     gene            complement(2829..4325)
                     /locus_tag="PLF56_08320"
     CDS             complement(2829..4325)
                     /locus_tag="PLF56_08320"
                     /EC_number="7.5.2.-"
                     /inference="COORDINATES: protein motif:HMM:NF012235.2"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_function: GO:0005524 - ATP binding [Evidence IEA];
                     GO_function: GO:0016887 - ATP hydrolysis activity
                     [Evidence IEA];
                     GO_function: GO:0042626 - ATPase-coupled transmembrane
                     transporter activity [Evidence IEA];
                     GO_function: GO:0140359 - ABC-type transporter activity
                     [Evidence IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="sugar ABC transporter ATP-binding protein"
                     /protein_id="HOQ53604.1"
                     /translation="MADVPLIEITGLVKQFGATQALKGVDFTLRSHSIHALLGQNGAG
                     KSTLIKVLSGLYPPTAGEVRVRGHQLGSPEAIRSMAFIHQDLGLVESMSVAENVALGT
                     SYSRRAGLISWREVRRLAVVALEHVQLDLDPDVLVADLTRAERSLVAIARALSADAPI
                     LVLDEPTASLPASDSQRLFDVLKNLREQGRGMIYVSHRLDEVFAISDTVTVFRDGRLV
                     HTGDIKAKTPQDLVVDIVGSKPKSYQSRFDAAQRPVVLSADRVLAGPAGPVSFEVHQG
                     EVVGLVGLTGAGHLQLGRAIAGAFPTHGGRFSLGGEPYAPATPAVAVDRGVGFVTSNR
                     MEEGMAPEMSVRENFLANLRPRGKGALSWNSPRRDAELGEQLTETFGVLPNNPEAPIA
                     TLSGGNQQKVMVGRWLSADRRLIVLEEPTAGVDVGAKADIYRLLEDALHAGLAVVLVS
                     TDFEEVASVAHRALVFVQGRMTEELAGDQLTVGNLTAASSGANLLATH"
BASE COUNT          729 a         1465 c         1571 g          710 t
ORIGIN      
        1 ggcgcaacac cactctgccc aaagatgcgc cgtcacgaaa gcaaaccgga cgacacgtcc
       61 acacatgtgc aggcaagcgc aggttcagcg caattgttct tcgagttcag agcgcatctg
      121 gtcgccgcca cccttgcgcc agcgatcgcg cagctcggcc aggtcagaca ccggacggcg
      181 accggtgacg atgccgccct cgtagtcaag caccatggcc gacagggcgt cacccttcga
      241 gaacgacgcg tccgagtcga ggcccttggc ggggtcgtcg acgaacgcgg ccaccatctg
      301 ctcggcgtac ttctggcagg tggtggcgtc tggcggcggc gcgccgaggc tcttcgcgct
      361 gtcaccccgg ctagggcggt ggagcctccc ccctaggggt gcgaaacgcc gcccgcaccg
      421 aagtgcgggc ggcgttggtt cagcgcaggg acggcctcac ttgccccaga tcgccttgta
      481 ggcgtcctgg tagccgaccg gatcccagaa gttgtcggcg gtggcgttct ccttggtcga
      541 cacgtggatc tgcggcacgt aaccggaggc cttttcgccg gcgaacgcgc ggttcagctc
      601 gtccacgatc tgccagccct cggagcgcag cggcgagggg acggtggcgg cctggaagtc
      661 gccggtgcgg atgcgggtca acgcggcggc gtcgccgtca ccggcaccca cgttctgcgg
      721 cgcggtctcg ttggtcttgc ccgcctgacg gaaggccgcg gtggcgttct cgaagtagac
      781 gtcgttgatc gcgaccgagt aggtccactt gtcacccagg ctgctcagca gggcgctggt
      841 ttcggtgggc atccggctgg ccacatcggc cagcgggatg ttgctgtact tgagcacgtt
      901 caccgacgag caggtctgca gctgcttctg gatcatctgg ctcttgccct cggcgaacgg
      961 aatggacgag tcggtgaaga tcaccacgcc ggccgtgccg ttggacttcg caatgacgta
     1021 gtcggcgctg atcttggcga cgtcctccac cttggtggtg acgttgctga acagcttggg
     1081 gctgtcactg gggccggggg tggccagcga ctgccagccg accagcggaa tgttggccgc
     1141 attggcctgc ttcacctgat cggcggtgct gttgggatcg aagccgccga tgatgatgcc
     1201 gtccggcttc agggtgagag cctggttgaa ggcgttctgg atgccggccg gggtgccctg
     1261 gccgtcgatc gtcttcagcg tccagccgat ggcggcggct gcgtccttca ggccggtctc
     1321 caccccggcc acgccgggat tctgcatcga ctgggcgacg tagacgatgg tcttgccgga
     1381 ggccgccttg gggccggtgg tcgggccgtc ccacttggcg tcggctgcgc tggccttgtc
     1441 gacggccgcc ttggaggagg cgatcacgtc ggtgcaggcg cccgcggctg tggccgccgc
     1501 actggtgggg ccggaggccg catcggagcc acgctggcaa cccgcgaggg cgagcgccgc
     1561 accgacggtg agcgcacagc caaccgcgag cggcttgctg agaagtctgg acatggtgag
     1621 cctttcgttg ttggtcgagc gagtttctag tggccggacg agggaccgga cggatcggct
     1681 gacgggtcga tcaccgcgct ggcggacgtg ctggggccga cggtgtcgac cagttcggcc
     1741 tcggccgcgt gcgccccggt gccaccgggt gaggccggag caccgctgtc gacggccttg
     1801 ttggcggcga taccggccat gatcttgcgc cgtgcactcc acccggccag gccgaccgcg
     1861 atcagcagcg tgacgccgtt gaacagcggg ttgacccaga agttggcgcc caactggccg
     1921 atgccggcca gaccgatcgc gagggtcacc acggcgacga tggtgccggc ggcgttgggc
     1981 cggccgggcc tgatcaccgt ggatccgagc agtgcggcga cgaaggcggg cagcatgtac
     2041 tccgaaccga ccgacgggtt gccgacgtgc tgttgcgagg cgagcagaat gccggccaca
     2101 cccaccagga acgaacaggc ggcgaaggcg tagaccgaat accgcctggt cggcaggccg
     2161 atcaggtcgg ccgcccgctg gttggagccg agaacgtaga agtagcgtcc cagcggcagg
     2221 cgctccagca cgatccacag caccaccgcg atcaccgtca cgtaccaggc cgcgatcggg
     2281 atgcccgcga tcttggagtc gtacaggtcg gtgaagccgg ccggcaggcc ttcgcccgtc
     2341 ggcaccaccc gggatccgtt cgtgacggcg ccggtgatcg cgtacagcac cgagccggta
     2401 cccagcgtgg ccacgaacga gtcgatcttg ccgagttcga ccagcaggcc gttgacgatg
     2461 ccgacgcagg tcagcaacac cagcacgatc accgctgcca gcatccagtt gatgttgttg
     2521 accaccatca ccatcatcag cacgtgcgcc atgcccaggg cgtagccgat ggacacgtcg
     2581 aacttgccgg tggcgatcgg gatcatggcg ccgagcgcca ggatcgccgg gatcgagttg
     2641 gcccccagaa tggcgttcag gttgttctgg gtgaggaacg tgcccggcat cagaatggcg
     2701 aacacggcga acaacagcac ggccaggacc agcaggccgt aggtgccgac gaaggaccct
     2761 ccgccccggc ggcggcgggc cggacgggtc ggggcggctg tggctgtggt cattgcgacg
     2821 actcctgatc agtgggtggc gagtaggttg gcgccggaac tggcggcggt gaggttgccg
     2881 acggtgagct gatcgccggc cagttcttcg gtcatccggc cctgcacgaa gaccagtgcg
     2941 cggtgggcga cgctggccac ctcctcgaag tcggtgctga ccagcacgac cgcgaggccg
     3001 gcgtgcagag cgtcctcgag aagccggtag atgtcggcct tggcgccgac gtcgacgccg
     3061 gctgtgggtt cctcgagcac gatcagccga cgatccgccg acagccagcg gcccaccatc
     3121 accttctgtt ggttgccgcc ggagagggtg gcgatcggcg cctcgggatt gttgggcagc
     3181 acgccgaagg tctcggtcaa ctgctcgccg agctcggcgt cccggcgcgg gctgttccag
     3241 gacagcgccc ctttgccgcg cgggcgcagg ttggcgagaa agttctcccg gacgctcatc
     3301 tccggcgcca tgccctcttc catccggttg gaggtcacga aaccgacccc gcggtcgacg
     3361 gccaccgcgg gcgtggccgg tgcatacggc tcaccaccca ggctgaaccg gccaccgtgg
     3421 gtggggaagg cgccggcgat cgcccggccc agttgcaggt gccccgctcc ggtgaggccg
     3481 acgaggccga cgacctcacc ctggtgcacc tcgaagctga ccggaccggc cggcccggcc
     3541 agcacccggt ccgcactcag caccacggga cgttgcgcgg cgtcgaaccg cgactggtac
     3601 gacttcggct tgctgccgac gatgtcgacc accaggtcct ggggcgtctt ggccttgatg
     3661 tcaccggtgt gcaccagccg accatcgcga aacaccgtca cggtgtcgct gatcgcgaag
     3721 acctcgtcga gccggtgcga gacgtagatc atcccgcggc cctgctcgcg caggttcttc
     3781 agcacgtcga acaggcgctg cgaatcggac gccggcagcg aggcggtcgg ctcgtccagc
     3841 accaggatcg gggcgtccgc cgacagggcg cgggcgatgg cgaccagtga gcgttcggcg
     3901 cgggtcaggt cggcgacgag cacgtcggga tcgagatcga gctgcacgtg ttcgagcgca
     3961 acgaccgcga gccggcgcac ctcacgccag ctgatcaggc cggcccgccg tgagtagctg
     4021 gtgcccagcg ccacgttctc ggcgacactc atcgactcca ccaggcccag gtcctgatgg
     4081 atgaaggcca tcgaccggat cgcctcgggt gagccgagtt gatggccacg cacccgcacc
     4141 tcgccggcag tcggcggata caggccggac agcaccttga tcagggtgga cttgccggca
     4201 ccgttctggc cgagcagggc atggatgctg tgcgaccgca gcgtgaaatc gacgcccttg
     4261 agtgcttggg tggcgccgaa ctgtttgacg aggcccgtga tctcgatcag gggaacatcg
     4321 gccatgaggt cctcaactct gagtgaaagc caacatactg gactgcaacc aagattctgg
     4381 actagagaac gacgcgatgc aacccttcag atcacattgc tcacgatcgg ctcagaacga
     4441 gcggccggcc aaggggttgc ggaacacccc ggcca
//
LOCUS       DAOSUG010000229         4450 bp    DNA     linear   ENV 10-JUN-2024
DEFINITION  MAG TPA_asm: Micropruina sp. isolate AMR_MDS_2195
            NODE_8401_length_4450_cov_3.420478, whole genome shotgun sequence.
ACCESSION   DAOSUG010000229 DAOSUG010000000
VERSION     DAOSUG010000229.1
DBLINK      BioProject: PRJNA925477
            BioSample: SAMN32864262
            Sequence Read Archive: SRR4236673
KEYWORDS    WGS; ENV; Metagenome Assembled Genome; MAG; Third Party Data; TPA;
            TPA:assembly.
SOURCE      Micropruina sp. (wastewater metagenome)
  ORGANISM  Micropruina sp.
            Bacteria; Bacillati; Actinomycetota; Actinomycetes;
            Propionibacteriales; Nocardioidaceae; Micropruina.
REFERENCE   1  (bases 1 to 4450)
  AUTHORS   Abdulkadir,N., Saraiva,J.P., Zhang,J., Stolte,S., Gillor,O.,
            Harms,H. and Rocha,U.
  TITLE     Genome-centric analyses of 165 metagenomes show that mobile genetic
            elements are crucial for the transmission of antimicrobial
            resistance genes to pathogens in activated sludge and wastewater
  JOURNAL   Microbiol Spectr 12 (3), e0291823 (2024)
   PUBMED   38289113
REFERENCE   2  (bases 1 to 4450)
  AUTHORS   Abdulkadir,N.
  TITLE     Direct Submission
  JOURNAL   Submitted (24-MAR-2023) Environmental Microbiology, Helmholtz
            Center for Environmental Research -UFZ, Permoserstr 15, Leipzig
            04318, Germany
COMMENT     The annotation was added by the NCBI Prokaryotic Genome Annotation
            Pipeline (PGAP). Information about PGAP can be found here:
            https://www.ncbi.nlm.nih.gov/genome/annotation_prok/
            
            ##Genome-Assembly-Data-START##
            Assembly Method        :: SPAdes v. 3.11.1
            Genome Representation  :: Full
            Expected Final Version :: Yes
            Genome Coverage        :: Not Applicable
            Sequencing Technology  :: Illumina MiSeq
            ##Genome-Assembly-Data-END##
            
            ##Genome-Annotation-Data-START##
            Annotation Provider               :: NCBI
            Annotation Date                   :: 05/04/2023 04:57:10
            Annotation Pipeline               :: NCBI Prokaryotic Genome
                                                 Annotation Pipeline (PGAP)
            Annotation Method                 :: Best-placed reference protein
                                                 set; GeneMarkS-2+
            Annotation Software revision      :: 6.5
            Features Annotated                :: Gene; CDS; rRNA; tRNA; ncRNA
            Genes (total)                     :: 2,776
            CDSs (total)                      :: 2,750
            Genes (coding)                    :: 2,714
            CDSs (with protein)               :: 2,714
            Genes (RNA)                       :: 26
            tRNAs                             :: 24
            ncRNAs                            :: 2
            Pseudo Genes (total)              :: 36
            CDSs (without protein)            :: 36
            Pseudo Genes (ambiguous residues) :: 0 of 36
            Pseudo Genes (frameshifted)       :: 2 of 36
            Pseudo Genes (incomplete)         :: 32 of 36
            Pseudo Genes (internal stop)      :: 2 of 36
            ##Genome-Annotation-Data-END##
FEATURES             Location/Qualifiers
     source          1..4450
                     /organism="Micropruina sp."
                     /mol_type="genomic DNA"
                     /submitter_seqid="NODE_8401_length_4450_cov_3.420478"
                     /isolate="AMR_MDS_2195"
                     /isolation_source="Activated sludge of municipal
                     wastewater treatment plants"
                     /db_xref="taxon:2737536"
                     /environmental_sample
                     /geo_loc_name="Canada"
                     /lat_lon="49.25 N 123.1 W"
                     /collection_date="2015-06-16"
                     /metagenome_source="wastewater metagenome"
                     /note="metagenomic"
     gene            222..1229
                     /locus_tag="PLF56_08325"
     CDS             222..1229
                     /locus_tag="PLF56_08325"
                     /inference="COORDINATES: protein motif:HMM:NF012742.2"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_function: GO:0000976 - transcription cis-regulatory
                     region binding [Evidence IEA];
                     GO_function: GO:0003700 - DNA-binding transcription factor
                     activity [Evidence IEA];
                     GO_function: GO:0043565 - sequence-specific DNA binding
                     [Evidence IEA];
                     GO_process: GO:0006351 - DNA-templated transcription
                     [Evidence IEA];
                     GO_process: GO:0006355 - regulation of DNA-templated
                     transcription [Evidence IEA];
                     GO_process: GO:0045892 - negative regulation of
                     DNA-templated transcription [Evidence IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="LacI family DNA-binding transcriptional
                     regulator"
                     /protein_id="HOQ53605.1"
                     /translation="MRPTLTEVAGLAGVSVSTASRAFSDPDRLNDETLARILGAAAQL
                     DYRGRARRGGDVASEPTTIGVIVPDIINPIFAEFVRAAQSHGWYRQQTMMLADTDSNP
                     QREREILAAIKHRVDGLVVVSPRQNVHDLLRLADGMPLVLVNRESDDCHTIITDATDG
                     LRQAIGYLARIGHRRFAYVQGPELSWTNQRRLATAIDTAEELRLQLEVLGWSAENIDG
                     GEAAAASVAASGATAALVHNDPMAIGVMRGLRGLGLNVPRDVSVVGVDNTMLTQLTSP
                     MLASVDIPMARAGVLAMDILFDAPDQPRHEVLTTQFMIGESTAPVRGWRPPQHDEKEI
                     R"
     gene            1226..2233
                     /locus_tag="PLF56_08330"
     CDS             1226..2233
                     /locus_tag="PLF56_08330"
                     /inference="COORDINATES: protein motif:HMM:NF014841.2"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_function: GO:0051287 - NAD binding [Evidence IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="NAD(P)-dependent oxidoreductase"
                     /protein_id="HOQ53606.1"
                     /translation="MIRVVVTDANLARVGDELGRLLAGHAELVWALDQDEQMAALAEA
                     DVLVGPAFSVAMAEAAPALRLVQVGGAGTDQIDPAAIRRHHELFGGRGARLQVANTFH
                     HEAAMAEYAIWAAVGLRRELARADHALRLGTWRSPVYDPALPLPPGLAGARVVLLGFG
                     HVGQAAWNAFRALGASGIAITGSGSVNAAEHGLTWAAASDRLVEACAESDVLLVSVPL
                     SERTRGVVGDEVLEALGSAGIVINLARGPVVDAEALYRRLSDGQLGGAALDVWYRYPS
                     APGEVCAPSEENFAALPNVLLTPHISGVALQTFLGRAGDIAHNVRALGEERPLINTVT
                     L"
     gene            2385..3758
                     /locus_tag="PLF56_08335"
     CDS             2385..3758
                     /locus_tag="PLF56_08335"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_002527146.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_function: GO:0022857 - transmembrane transporter
                     activity [Evidence IEA];
                     GO_process: GO:0055085 - transmembrane transport [Evidence
                     IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="MFS transporter"
                     /protein_id="HOQ53607.1"
                     /translation="MSQSTTHPHVADPTADVPDSEIRRAAAAGLIGTALELYDFVIYA
                     TASALVFGTIFFPNMSPTAALIGSFSAYAVGFLARPVGGLFFARLGDKLGRKFILVAT
                     LFLMGISTLAIGLLPTYSQVGILAPILLLVCRMAQGFGAGAEQAGGATLLTETARIGQ
                     RGRYASLVMVGAAPGTALGAGAWALIQLLPAEQVLSWGWRAVFLSSIVVTITAYILRR
                     TLKETPVFTEIKAEHSAAQAPVREVLTKGKLPVWLVTFMTIGVSVQSYTYQVFMASYL
                     KTQIGLSGGTVAEILLVGALAGGVSALVMGRLSDRFGRRPVYLTILGLLILLPVPTFF
                     ALNTGSVAWAYAAMIVGFVLAGQGAVAVSMSYFPELFGSRYRYAGVTLGREFAAVLGG
                     GFAPLIAVALLNVTGSWVPIAVYMMVAMGISFIAALRCPETLNRDLTVPENATDPGAA
                     PAPGYVR"
     gene            complement(3954..>4450)
                     /locus_tag="PLF56_08340"
     CDS             complement(3954..>4450)
                     /locus_tag="PLF56_08340"
                     /EC_number="4.1.1.32"
                     /inference="COORDINATES: protein motif:HMM:NF013019.2"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_function: GO:0004611 - phosphoenolpyruvate
                     carboxykinase activity [Evidence IEA];
                     GO_process: GO:0006094 - gluconeogenesis [Evidence IEA]"
                     /codon_start=3
                     /transl_table=11
                     /product="phosphoenolpyruvate carboxykinase
                     domain-containing protein"
                     /protein_id="HOQ53608.1"
                     /translation="RTAAAEGTVGELRRDPFAMLPFCGYNMADYWAHWLKVGEGLGEH
                     APRVFQVNWFRKDSDGRFLWPGFGENSRAIEWALRRINGEVDAVDAINGRQPRREDLN
                     VEGIEITGEQLDELFHLDPEAWGKEADLTEEYFAQFGDKLPAALSDELDALRGRIAAA
                     TASQ"
BASE COUNT          651 a         1537 c         1499 g          763 t
ORIGIN      
        1 ttagttgtga cggcggccgc gcgagccagg tggactgccg gacgaatccc gcccggaggt
       61 tctgcccgga tccaccgacc ggcgttgctg tgcgactcgc tgcggacgcc ctggcgttgc
      121 cgggcgcgca tcgcagtccg ggcgcccctg tggcaaaacg ttgcaaagtt gtcgcaagat
      181 gcgcaatact tgccgggtcg acgaggacag gaggagtcgc gatgcgtcca acgctcaccg
      241 aggtggccgg actggccggt gtgtcagtca gcaccgcatc ccgtgccttc agcgaccccg
      301 accggctcaa cgacgaaacc ctggccagga ttctgggggc tgcggcgcaa ctcgactatc
      361 gcggacgagc caggcgaggt ggcgacgtgg ccagcgagcc gaccacgatc ggcgtcatcg
      421 tgcccgacat catcaacccg atcttcgccg agttcgtccg ggccgcgcag agccacggct
      481 ggtaccgcca gcagaccatg atgctggccg acaccgacag caatcctcag cgcgagcgcg
      541 agattctcgc cgccatcaag cacagggtgg acgggctggt ggtggtctcg ccacgacaga
      601 acgtccacga cctgttgcgg ctggccgacg gcatgccgct ggtgctggtc aaccgcgaga
      661 gcgacgactg ccacaccatc atcaccgacg ccaccgacgg cctgcgccaa gcgatcggct
      721 acctggctcg gatcgggcat cgccgcttcg cctacgtgca gggccccgaa ctgtcgtgga
      781 cgaaccagcg ccggctggcc accgctatcg acacggccga agaactccgc ctgcagctgg
      841 aggtgctcgg ctggtcggcc gagaacatcg acggcggcga ggcggcggcg gccagcgtgg
      901 cggcgtccgg ggcgacggcg gcgctggtcc acaacgatcc gatggcgatc ggcgtgatgc
      961 gcgggctgcg cggcctcggg ctgaacgtgc ctcgcgacgt cagcgtggtc ggcgtcgaca
     1021 acaccatgct cacccagctc acctcgccga tgctggccag cgtcgacatt ccgatggccc
     1081 gcgccggcgt gctggccatg gacattctct tcgatgcacc cgatcagccg cggcacgagg
     1141 tgctgaccac ccagttcatg atcggcgaga gcaccgcccc cgtgcggggc tggcgtccgc
     1201 cgcaacacga tgagaaggag atccgatgat ccgcgttgtt gtcaccgatg ccaacctggc
     1261 ccgggtgggc gacgaactgg gccggctgtt ggcggggcac gccgagctgg tctgggctct
     1321 cgaccaggac gagcagatgg cggccctggc cgaggcggac gtgctggtcg gccccgcttt
     1381 cagcgtcgcc atggcggagg ccgcgcccgc gcttcggctg gtgcaggtcg gcggcgccgg
     1441 caccgatcag atcgatccgg cggcgatccg gcggcaccac gaactgttcg gtggccgggg
     1501 cgcccgcctg caagtggcca acacctttca ccacgaggcc gccatggccg agtatgcgat
     1561 ctgggccgcc gtcggccttc gtcgagagct ggcccgtgcc gaccacgcgc tgcggctggg
     1621 cacgtggcgc tcaccggtct acgacccggc actcccgctg ccgccgggcc tggcgggggc
     1681 acgcgtggtg ctgctcggct tcgggcatgt gggccaagcc gcctggaacg ccttccgtgc
     1741 attgggtgcg tccggtattg cgatcaccgg cagtggcagc gtgaatgccg ccgagcacgg
     1801 cctgacctgg gcggcagcgt ccgaccgcct ggtcgaggcc tgcgctgaat cggacgtgct
     1861 cctggtgtcc gtccccttgt cggaacgcac tcggggggtc gtgggtgacg aggtgctgga
     1921 ggcgctgggc agtgccggca tcgtgatcaa cctggcgcgc ggcccggtgg tcgacgccga
     1981 ggcgctgtac cggcgactca gcgacggaca actgggtggt gccgccctcg atgtctggta
     2041 ccgctacccc agcgctcccg gcgaggtttg tgccccgtcc gaagagaact tcgccgcact
     2101 gcccaacgtg ctactcaccc cccacatttc cggtgtcgcg ctccagacct ttctgggccg
     2161 cgccggtgac attgcccaca atgtgcgtgc actcggggag gaaaggcccc tgatcaacac
     2221 ggtcacgctc tgacgtcggg tccctttccg cccgcctgac caccacccgt ccgcctctcc
     2281 ccagaccggg ggaggcccgc accgcagtgc ccgcacaccc ccttcgtccg accctgaacc
     2341 gattgattcc acccgaatcg tcccaacctc aaagaggagg catcatgtcc cagtcaacga
     2401 cccacccgca cgtggccgac ccgaccgccg acgttcccga ttccgagatc cggcgggccg
     2461 ccgcggccgg cctgatcggc acggccctgg agctctacga cttcgtcatc tacgccaccg
     2521 ccagcgcgct ggtgttcggc accatcttct ttcccaacat gtcacccacc gcggccctga
     2581 tcggttcgtt cagcgcctac gccgtcggct tcctggcccg gccggtcggt ggtctgttct
     2641 tcgcacggct cggtgacaag ctcggacgca agttcattct ggttgccacg ctgttcctga
     2701 tgggcatctc gacactggcc atcggcctgc tgcccaccta ttcacaggtt ggcattttgg
     2761 cgccgatcct gctgctggtg tgccggatgg cgcagggttt cggtgccggg gccgagcagg
     2821 ccggcggtgc gaccctgctg accgagacgg ccaggatcgg ccagcgcggt cgctacgcgt
     2881 cgctggtcat ggtgggcgca gcgcccggta ccgcgctcgg cgccggtgcc tgggcgttga
     2941 tccagttgtt gcccgccgag caggtgctca gctggggctg gcgggcggtg ttcctgtcgt
     3001 cgatcgtggt caccatcacc gcctacattc tgcgccgcac cctgaaggag accccggtct
     3061 tcaccgagat caaggccgag cacagtgccg cccaggcacc ggtgcgcgag gtgctgacca
     3121 agggcaagct gccggtgtgg ttggtcacgt tcatgaccat cggcgtcagc gtgcagtcat
     3181 acacctatca ggtcttcatg gcgtcgtacc tgaagaccca gatcggactc agcggtggaa
     3241 cggtggccga gattctgctg gtcggcgcgc tggccggcgg tgtgagcgcg ttggtgatgg
     3301 gtcggctcag tgaccggttc ggacgccgtc cggtctacct cacgattctg ggcctgctga
     3361 ttctgttgcc cgtgccgacg ttcttcgcac tcaacaccgg atcggtggcg tgggcctatg
     3421 ccgccatgat cgtcggcttc gtgctggccg gccagggcgc agtagctgtc tcgatgagct
     3481 actttcccga gctgttcggc agccgctacc gctacgccgg cgtcacgctg ggccgcgagt
     3541 tcgccgccgt gcttggtggc ggtttcgctc ccctgatcgc ggtcgcactg ctgaacgtca
     3601 ccggcagttg ggtgccgatc gcggtctaca tgatggtggc catgggcatt tcgttcattg
     3661 ccgccctgcg atgccccgag acgctcaacc gtgacttgac cgttccggag aatgccaccg
     3721 atccgggcgc ggcccccgct ccgggttacg tgcgctgaac gagagctgcg accccggacg
     3781 ctcccggtgt gccggtccac cgctgaggtt gaccggcaca cgggcgtccg gacgagagcg
     3841 tggcccgccc gtgctcagaa aacgttgatc aatcgctggt cgagcgggtc gtggcccaat
     3901 gcgagcaggc gtctcgaccc gctccaccag cgaatcacgc gttggatcgg cgcttactgc
     3961 gacgcggttg cggcggcgat tctgcctcgc aacgcgtcga gttcgtcgct cagcgcggcg
     4021 ggcagcttgt caccgaactg ggcgaagtat tcctcggtca ggtcggcctc tttaccccag
     4081 gcctcagggt cgaggtggaa gagttcgtcc aactgctcgc cggtgatctc gatgccctcg
     4141 acgttgagat cctcgcggcg gggctggcgg ccgttgatgg cgtccacggc gtccacttcg
     4201 ccgttgatgc ggcgcagcgc ccactcgatg gcgcgggagt tctcgccgaa accgggccac
     4261 aggaatcgtc cgtcggaatc cttgcggaac cagttcacct ggaaaacccg cggcgcgtgc
     4321 tcaccgaggc cctcgccgac cttcagccag tgcgcccagt agtcggccat gttgtagccg
     4381 cagaacggca gcatggcgaa cgggtcgcgg cgcagctctc cgaccgtgcc ctcggcggcg
     4441 gcggtgcgct
//
LOCUS       DAOSUG010000230         4436 bp    DNA     linear   ENV 10-JUN-2024
DEFINITION  MAG TPA_asm: Micropruina sp. isolate AMR_MDS_2195
            NODE_8425_length_4436_cov_5.547135, whole genome shotgun sequence.
ACCESSION   DAOSUG010000230 DAOSUG010000000
VERSION     DAOSUG010000230.1
DBLINK      BioProject: PRJNA925477
            BioSample: SAMN32864262
            Sequence Read Archive: SRR4236673
KEYWORDS    WGS; ENV; Metagenome Assembled Genome; MAG; Third Party Data; TPA;
            TPA:assembly.
SOURCE      Micropruina sp. (wastewater metagenome)
  ORGANISM  Micropruina sp.
            Bacteria; Bacillati; Actinomycetota; Actinomycetes;
            Propionibacteriales; Nocardioidaceae; Micropruina.
REFERENCE   1  (bases 1 to 4436)
  AUTHORS   Abdulkadir,N., Saraiva,J.P., Zhang,J., Stolte,S., Gillor,O.,
            Harms,H. and Rocha,U.
  TITLE     Genome-centric analyses of 165 metagenomes show that mobile genetic
            elements are crucial for the transmission of antimicrobial
            resistance genes to pathogens in activated sludge and wastewater
  JOURNAL   Microbiol Spectr 12 (3), e0291823 (2024)
   PUBMED   38289113
REFERENCE   2  (bases 1 to 4436)
  AUTHORS   Abdulkadir,N.
  TITLE     Direct Submission
  JOURNAL   Submitted (24-MAR-2023) Environmental Microbiology, Helmholtz
            Center for Environmental Research -UFZ, Permoserstr 15, Leipzig
            04318, Germany
COMMENT     The annotation was added by the NCBI Prokaryotic Genome Annotation
            Pipeline (PGAP). Information about PGAP can be found here:
            https://www.ncbi.nlm.nih.gov/genome/annotation_prok/
            
            ##Genome-Assembly-Data-START##
            Assembly Method        :: SPAdes v. 3.11.1
            Genome Representation  :: Full
            Expected Final Version :: Yes
            Genome Coverage        :: Not Applicable
            Sequencing Technology  :: Illumina MiSeq
            ##Genome-Assembly-Data-END##
            
            ##Genome-Annotation-Data-START##
            Annotation Provider               :: NCBI
            Annotation Date                   :: 05/04/2023 04:57:10
            Annotation Pipeline               :: NCBI Prokaryotic Genome
                                                 Annotation Pipeline (PGAP)
            Annotation Method                 :: Best-placed reference protein
                                                 set; GeneMarkS-2+
            Annotation Software revision      :: 6.5
            Features Annotated                :: Gene; CDS; rRNA; tRNA; ncRNA
            Genes (total)                     :: 2,776
            CDSs (total)                      :: 2,750
            Genes (coding)                    :: 2,714
            CDSs (with protein)               :: 2,714
            Genes (RNA)                       :: 26
            tRNAs                             :: 24
            ncRNAs                            :: 2
            Pseudo Genes (total)              :: 36
            CDSs (without protein)            :: 36
            Pseudo Genes (ambiguous residues) :: 0 of 36
            Pseudo Genes (frameshifted)       :: 2 of 36
            Pseudo Genes (incomplete)         :: 32 of 36
            Pseudo Genes (internal stop)      :: 2 of 36
            ##Genome-Annotation-Data-END##
FEATURES             Location/Qualifiers
     source          1..4436
                     /organism="Micropruina sp."
                     /mol_type="genomic DNA"
                     /submitter_seqid="NODE_8425_length_4436_cov_5.547135"
                     /isolate="AMR_MDS_2195"
                     /isolation_source="Activated sludge of municipal
                     wastewater treatment plants"
                     /db_xref="taxon:2737536"
                     /environmental_sample
                     /geo_loc_name="Canada"
                     /lat_lon="49.25 N 123.1 W"
                     /collection_date="2015-06-16"
                     /metagenome_source="wastewater metagenome"
                     /note="metagenomic"
     gene            <1..292
                     /locus_tag="PLF56_08345"
     CDS             <1..292
                     /locus_tag="PLF56_08345"
                     /EC_number="1.10.3.-"
                     /inference="COORDINATES: protein motif:HMM:NF014386.2"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_component: GO:0016020 - membrane [Evidence IEA]"
                     /codon_start=2
                     /transl_table=11
                     /product="cytochrome d ubiquinol oxidase subunit II"
                     /protein_id="HOQ53609.1"
                     /translation="ADGWAFVGTAVSIVVVAIGIFLTMYPDLGFDNSAAERPLNIVTA
                     SATPYTLTIMTWAAAVFVPIVLGYQAWSYWVFRRRLSTANIPDEAKESEPVG"
     gene            455..1525
                     /locus_tag="PLF56_08350"
     CDS             455..1525
                     /locus_tag="PLF56_08350"
                     /inference="COORDINATES: protein motif:HMM:NF016143.2"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="neutral zinc metallopeptidase"
                     /protein_id="HOQ53610.1"
                     /translation="MSLGPTDGSQQRPLPTTPPAASDPAALGPIEQPAPQQSHTQQPA
                     GSPPPHVQQAPAWQQSYVPQPAAWQPPAPAWRAAPPKPRGPSPVVFIVVLLLAVGVTL
                     AGLQLLPIPTASPTGETRPGSSASPTPSASTDAPTTGQHPDLTENKIYPMSVSGSCPS
                     VHTPSSMAAYKKQVGELLTCLAGIFDPLIERAGAVPIAVGHVFYGKQVDTPCGGEADA
                     YAFYCESDRTIYLSEQVYEDSQYARLSVADTVIHEYGHHVQEMAGIFEAADKLDESQS
                     VIVRREELQVFCWTYYVFASVPTFELTATDRAFIADLWSQTSDPDGHGTVTAQKYWGA
                     RGLSGRNLGACNTWSVPKSKVR"
     gene            1572..1817
                     /locus_tag="PLF56_08355"
     CDS             1572..1817
                     /locus_tag="PLF56_08355"
                     /inference="COORDINATES: protein motif:HMM:NF040429.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="DUF6457 domain-containing protein"
                     /protein_id="HOQ53611.1"
                     /translation="MPDKEDLSVHDPWVDAVCAALQVPREVVDIDAVLGLAGRVAHRT
                     ARPMAPVSTFLLGYALGCGGGTLDDVRARILAVRTDG"
     gene            2680..4317
                     /locus_tag="PLF56_08360"
     CDS             2680..4317
                     /locus_tag="PLF56_08360"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_016668952.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="NAD(P)-binding protein"
                     /protein_id="HOQ53612.1"
                     /translation="MTEPLRLDMTHLPDLAATASRVGPVRTRMPVYVDLLPPCNNACP
                     AGENIQEWLRLVKANSDEAAWRELTRNNPFPAIHGRVCYHPCETACNRIELDGSVSIH
                     SVERYLGDLAIERGWTFRRTRPATGRRVLVVGAGPSGLSAAYQLAMLGHDVEVRDASA
                     QPGGMMRYGIPKYRLPRDVLDAEVARIVDLGVRIVTDHRVTDLLAEQADGGFDAVFVA
                     IGAHLSKRVDIPTRDASRIVDAVSFLRDVASGERPAIGRRVAVYGGGNTAMDAARVAR
                     RLGAEESIVVYRRTQEQMPAHPSEREEALAEGVQMNWLRTISSLDSHDMTVEIMELDD
                     EGRAVGTGRFETLQADTVILALGQETDSDFLKAIPGMRFDRDVLQVDRTTLMTDVPGI
                     FAGGDAVPSERTVTVGVGHGKRAARMIDAWLDNRPPVTAPKHEIAGFDTLNLWYFGDN
                     RRSSQPELNPANRVTDFDEIHGGLDTGQAHVEASRCLSCGNCFECDGCLGACPEDAIV
                     KLGKGFRYRFDYEKCTGCATCYEQCPVHAIEMIPEHS"
BASE COUNT          730 a         1542 c         1476 g          688 t
ORIGIN      
        1 ggcggacggc tgggccttcg tcggcacggc cgtctcgatc gtcgtggtcg ccatcgggat
       61 cttcttgacc atgtaccccg acctgggctt cgacaactcg gcagcggagc gtccgctgaa
      121 catcgtgacc gcctcggcca cgccctacac cctcaccatc atgacttggg cagcggcggt
      181 gttcgtaccg atcgtgctcg gctaccaggc ctggtcgtac tgggtgttcc ggcgccggct
      241 gagcaccgcc aacattccgg acgaggcgaa ggagtccgag ccggtcgggt aagcggggcc
      301 acccctgggc tcaaccctga ttcacgccgc cccggttgac gccgggcacc ctgatggcgg
      361 acgagtcccg gccgccgggg tgctccggcc gatcgcagcc actccgatcg ccgcctgacc
      421 accgcgattg accgcgtgcc atatcgttga ggccgtgagc ctcgggccga cggacggcag
      481 ccagcagcgg ccgttgccga cgacgccgcc ggcggcgtcg gacccggccg ctctcgggcc
      541 catcgaacag ccggcgccgc agcagtccca cacgcagcag ccggcagggt cgccgccacc
      601 ccacgtccag caggcgcccg cctggcagca gtcctacgtt ccacagccgg cagcctggca
      661 acctccggct cctgcgtggc gggcggcacc gccgaagcct cgtggcccca gcccggtcgt
      721 cttcatcgtc gtgctgctgc tcgcggtcgg cgtgaccctg gcgggcctgc aactgcttcc
      781 gatccccacg gcgtcgccga cgggcgaaac ccggcccggc tcgtccgcct cgcccacccc
      841 gtcggcctcc accgacgctc cgacgactgg gcagcatccc gacctgaccg agaacaagat
      901 ctacccgatg agcgtcagtg gcagctgccc ctcggtgcac acaccatcgt cgatggcggc
      961 gtacaagaag caggtcggcg agttgctcac ctgcctggcc ggcatcttcg acccgttgat
     1021 cgagcgggcc ggcgccgtgc cgatcgcggt tggccacgtc ttctacggca aacaggtcga
     1081 cactccctgc ggcggcgagg ccgacgcgta cgccttctac tgcgagagcg atcgcacgat
     1141 ctacctctcc gaacaggtct acgaggattc gcagtacgcc cgtttgtcgg tggccgacac
     1201 ggtgatccac gagtacggcc atcacgtgca agaaatggcc ggcatcttcg aggcggccga
     1261 caagctcgac gagagccagt cggtgatcgt gcgacgcgaa gaactgcagg tgttctgctg
     1321 gacctattac gtgttcgcct cggttccgac cttcgagttg accgcgaccg atcgagcctt
     1381 catcgccgac ctctggtcac agaccagcga cccggacggc cacggcaccg tcacggcaca
     1441 gaaatactgg ggcgcccgcg ggctgagcgg caggaacctg ggtgcctgca acacctggag
     1501 cgtgccgaag agcaaggtgc gatgaggcat cggccggcca tttatcggtc ggccgtagga
     1561 gaggagaagc ggtgcccgac aaggaagatc tgagcgtgca cgatccctgg gtcgatgccg
     1621 tgtgtgccgc cctgcaggtg ccccgcgagg tcgtcgacat cgacgccgtg ctcggccttg
     1681 ccggacgcgt cgcgcaccgc accgcccgtc cgatggcccc ggtcagcacc tttctgctcg
     1741 gctacgccct cggctgcggc ggtggcacgt tggacgacgt gcgcgccagg attctggcgg
     1801 tgcgcaccga cggctgagcc ggttgtggga acccattgat cgagcctgtc gattgatcga
     1861 gcctgtcgac atcggggcac gtgggaggtc tcccggcgca tccctccacc gtggatcgag
     1921 attgtcgaga tcccggcagg caggaggtcg cggcgggccg gtctcgaagg gccagtcggg
     1981 gtgatcggac ttgctggcgg tgtcgagatc ctccgatggc ggggaggcgt cgagtgtcag
     2041 gttgcaacac gagagccgcg agggttccga ccacccggtt tcaggttgca acaagagagc
     2101 cgcgaggctt cccgccaccc ggtgtgagct ttcgacgtca cggcgccgcc ctcacggtgc
     2161 ttgctattcg atcgggccag acctcgatac caaatccacc actcgataac gctttgaacg
     2221 attcaatggc cccgattcac gaatcgagcg gtggatttgg tatttcgtca aacgatgaac
     2281 tgctgtcaac cccgcggggc tcgaccagcg agtaacgctc gacccggtac caaacgcagg
     2341 tgcgaagcgg agtgggggtg caccccgtca gggtgtggtc gggcgtaggc aaacttctcg
     2401 gcggagcctg cggagccgag aaggcttgcc ggggcgggac ggggtgcatc cccactccgc
     2461 ggcaacagac gcaacgaaca gagcgcgggc atgagggctc gacacgctcg accaacggag
     2521 tgcaagggcg cgggaagatc ccggcgttcg ccgggaacgg atgtctcgac cagcggtacg
     2581 gggtacgtgg ctcgaccacg gctttcccgg atcacctgtg caggcagcgt cccgcagcta
     2641 cattcagaag agttctgcac tcgtcgtcga gaggacgcca tgaccgaacc gctgcgtctg
     2701 gacatgaccc accttccgga cctggcggcc accgcgtccc gggtcgggcc cgtgcgcacc
     2761 cgcatgccgg tttacgtcga cctgctgccg ccctgcaaca acgcctgccc cgccggcgag
     2821 aacattcagg agtggctgcg cctggtcaag gccaactccg acgaggccgc ctggcgcgag
     2881 ttgacccgca acaatccctt ccccgccatt cacggacggg tctgctacca cccctgcgaa
     2941 accgcctgca accgcatcga actcgacggc tcggtcagca tccattccgt cgagcgatac
     3001 ctgggcgacc tggccatcga gcggggctgg acgttccgcc gcacccgtcc cgccaccgga
     3061 cgccgggtgc tggtggtggg tgccggaccg tccgggctgt cggcggccta ccagctcgcc
     3121 atgctcggcc acgacgtcga ggtgcgggac gcctccgcac agcccggcgg catgatgcgc
     3181 tacggcattc ccaagtaccg gctgccccgc gacgtgctcg acgccgaggt ggcgcgcatc
     3241 gtcgacctcg gggtacgcat cgtcaccgac caccgggtca cggacctgct cgccgagcag
     3301 gccgacggcg gcttcgacgc ggtgttcgtg gcgatcggag cgcacctgtc caagcgggtc
     3361 gacattccga cccgcgatgc gtcacggatc gtcgacgcgg tcagttttct gcgcgacgtg
     3421 gccagcggcg aacgtcccgc gatcggacgc cgggtcgccg tctacggcgg cggcaacacc
     3481 gcgatggacg ccgcccgcgt cgcccgccgg ctgggcgccg aggagtcgat cgtggtctac
     3541 cggcgcaccc aggagcagat gcccgcgcac ccgtccgagc gcgaggaggc gctggccgag
     3601 ggcgtgcaga tgaactggct gcgcaccatc tcgtccctgg attcccacga catgaccgtc
     3661 gagatcatgg agttggacga cgagggccgc gcggtgggca ccggccggtt cgagacgctg
     3721 caggccgata cggtgatcct ggccctcggc caggagaccg attcggactt tctcaaggcg
     3781 atccccggca tgcggttcga ccgcgacgtg ctgcaggtcg accgcaccac cttgatgacc
     3841 gacgtgcccg gcatcttcgc cggcggcgac gcggtgccgt ccgagcgcac cgtgacggtg
     3901 ggcgtcggtc acggcaaacg ggcggcgcgc atgatcgacg cgtggctgga caaccggccg
     3961 ccggtgaccg cacccaagca cgagatcgcc ggcttcgaca cgctcaatct gtggtacttc
     4021 ggcgacaacc gccgcagcag ccagcccgag ctgaacccgg ccaaccgggt caccgacttc
     4081 gatgagatcc acggcggtct cgacaccggg caggcccacg tcgaggcgtc gcggtgcttg
     4141 tcgtgcggca actgcttcga gtgcgacggg tgcctcggcg cgtgtcccga ggacgccatc
     4201 gtcaagctcg gtaagggctt tcgctaccgc ttcgactacg agaaatgcac cggctgtgcg
     4261 acctgctacg agcagtgccc cgtacacgcc atcgagatga ttccggagca ctcatgagag
     4321 ccatcatcga cggcaacgag gccgcggcct cggttgccta ccgattgaac gagctgtgca
     4381 gcatctaccc gatcacgccg agttcgacga tggccgagct ggcggacgag tggtcg
//
LOCUS       DAOSUG010000231         4426 bp    DNA     linear   ENV 10-JUN-2024
DEFINITION  MAG TPA_asm: Micropruina sp. isolate AMR_MDS_2195
            NODE_8442_length_4426_cov_3.499199, whole genome shotgun sequence.
ACCESSION   DAOSUG010000231 DAOSUG010000000
VERSION     DAOSUG010000231.1
DBLINK      BioProject: PRJNA925477
            BioSample: SAMN32864262
            Sequence Read Archive: SRR4236673
KEYWORDS    WGS; ENV; Metagenome Assembled Genome; MAG; Third Party Data; TPA;
            TPA:assembly.
SOURCE      Micropruina sp. (wastewater metagenome)
  ORGANISM  Micropruina sp.
            Bacteria; Bacillati; Actinomycetota; Actinomycetes;
            Propionibacteriales; Nocardioidaceae; Micropruina.
REFERENCE   1  (bases 1 to 4426)
  AUTHORS   Abdulkadir,N., Saraiva,J.P., Zhang,J., Stolte,S., Gillor,O.,
            Harms,H. and Rocha,U.
  TITLE     Genome-centric analyses of 165 metagenomes show that mobile genetic
            elements are crucial for the transmission of antimicrobial
            resistance genes to pathogens in activated sludge and wastewater
  JOURNAL   Microbiol Spectr 12 (3), e0291823 (2024)
   PUBMED   38289113
REFERENCE   2  (bases 1 to 4426)
  AUTHORS   Abdulkadir,N.
  TITLE     Direct Submission
  JOURNAL   Submitted (24-MAR-2023) Environmental Microbiology, Helmholtz
            Center for Environmental Research -UFZ, Permoserstr 15, Leipzig
            04318, Germany
COMMENT     The annotation was added by the NCBI Prokaryotic Genome Annotation
            Pipeline (PGAP). Information about PGAP can be found here:
            https://www.ncbi.nlm.nih.gov/genome/annotation_prok/
            
            ##Genome-Assembly-Data-START##
            Assembly Method        :: SPAdes v. 3.11.1
            Genome Representation  :: Full
            Expected Final Version :: Yes
            Genome Coverage        :: Not Applicable
            Sequencing Technology  :: Illumina MiSeq
            ##Genome-Assembly-Data-END##
            
            ##Genome-Annotation-Data-START##
            Annotation Provider               :: NCBI
            Annotation Date                   :: 05/04/2023 04:57:10
            Annotation Pipeline               :: NCBI Prokaryotic Genome
                                                 Annotation Pipeline (PGAP)
            Annotation Method                 :: Best-placed reference protein
                                                 set; GeneMarkS-2+
            Annotation Software revision      :: 6.5
            Features Annotated                :: Gene; CDS; rRNA; tRNA; ncRNA
            Genes (total)                     :: 2,776
            CDSs (total)                      :: 2,750
            Genes (coding)                    :: 2,714
            CDSs (with protein)               :: 2,714
            Genes (RNA)                       :: 26
            tRNAs                             :: 24
            ncRNAs                            :: 2
            Pseudo Genes (total)              :: 36
            CDSs (without protein)            :: 36
            Pseudo Genes (ambiguous residues) :: 0 of 36
            Pseudo Genes (frameshifted)       :: 2 of 36
            Pseudo Genes (incomplete)         :: 32 of 36
            Pseudo Genes (internal stop)      :: 2 of 36
            ##Genome-Annotation-Data-END##
FEATURES             Location/Qualifiers
     source          1..4426
                     /organism="Micropruina sp."
                     /mol_type="genomic DNA"
                     /submitter_seqid="NODE_8442_length_4426_cov_3.499199"
                     /isolate="AMR_MDS_2195"
                     /isolation_source="Activated sludge of municipal
                     wastewater treatment plants"
                     /db_xref="taxon:2737536"
                     /environmental_sample
                     /geo_loc_name="Canada"
                     /lat_lon="49.25 N 123.1 W"
                     /collection_date="2015-06-16"
                     /metagenome_source="wastewater metagenome"
                     /note="metagenomic"
     gene            complement(<1..512)
                     /locus_tag="PLF56_08365"
     CDS             complement(<1..512)
                     /locus_tag="PLF56_08365"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_008355047.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_function: GO:0022857 - transmembrane transporter
                     activity [Evidence IEA];
                     GO_process: GO:0055085 - transmembrane transport [Evidence
                     IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="MFS transporter"
                     /protein_id="HOQ53613.1"
                     /translation="MPRVRNPLVVSLLGLRGNGRACVYTEPMWGLSMALVLPYASVFM
                     LALGVRDQQIGLLATISMLSQVVFGLLSGVITDKLGRRMTTAAFDIVAWVIPCLIWAF
                     AQNFWWFLVASVINGAWQVTQNSWDCLLVEDVPRAELTKVYSLVKVAADCSALFAPIA
                     AVLVAQFGLVP"
     gene            complement(638..1021)
                     /locus_tag="PLF56_08370"
     CDS             complement(638..1021)
                     /locus_tag="PLF56_08370"
                     /inference="COORDINATES: protein motif:HMM:NF020397.2"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="DUF1801 domain-containing protein"
                     /protein_id="HOQ53614.1"
                     /translation="MTTTDPRVDDYLATMPAWQADFARRVRVLLHEADPELEETIKRT
                     RQPYFVLQGNVAAFLGTKDHLNVFIYDPIAPDPTGLINQGRGNATARSIQLFEGDELD
                     EAALLALFRAVIANNRAGGWRRLAR"
     gene            complement(1284..>2131)
                     /locus_tag="PLF56_08375"
     CDS             complement(1284..>2131)
                     /locus_tag="PLF56_08375"
                     /inference="COORDINATES: protein motif:HMM:NF013070.2"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_process: GO:0006355 - regulation of DNA-templated
                     transcription [Evidence IEA]"
                     /codon_start=3
                     /transl_table=11
                     /product="PRD domain-containing protein"
                     /protein_id="HOQ53615.1"
                     /translation="AMQVLKVFNNNVVLSLDELGPEVVITGRGVGFQARPGDPIDPAL
                     IVRRFVPADNPDSIAQVLADIPPERLALVETLFAQAMRELGSLMPPLAVVAVADHVHQ
                     AIERVARGEVMEYPMRAEVAHLHPVELEVAEALLAKLNRELDAPLPQGEAIALAMHLF
                     HAVTGSPSMEQTFVQSKLIRQIFDTVQQAYGDEFDPDSIDAARFATHLRYFFARAQAG
                     RQLDGGIPDVAVLVASGKGRAQQVALRIKSLLELRLQHPVSEDEVTYLTMHVARMEQG
                     MRTRV"
     assembly_gap    2132..2231
                     /estimated_length=100
                     /gap_type="within scaffold"
                     /linkage_evidence="paired-ends"
     gene            complement(<2232..3595)
                     /locus_tag="PLF56_08380"
     CDS             complement(<2232..3595)
                     /locus_tag="PLF56_08380"
                     /EC_number="3.2.1.-"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_016290021.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_function: GO:0016798 - hydrolase activity, acting on
                     glycosyl bonds [Evidence IEA];
                     GO_process: GO:0005975 - carbohydrate metabolic process
                     [Evidence IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="glycoside hydrolase family 1 protein"
                     /protein_id="HOQ53616.1"
                     /translation="MTTTFPDGFLWGGATAANQLEGAYDADGKGLSIQDVMPRGIVAP
                     PTDEPTPDNLKHEGIDHYHRYAEDIALFAEMGFKVYRFSIAWSRIFPNGDELTPNEEG
                     LAFYDRLLDELEKYGIEPLVTISHYETPLHLAKTYDGWVDRRLIGFYERYARVLFERF
                     GDRVKYWLTFNEINSVLHAPLMSGGIWTPKAELSEADLYQAIHHELVASASATKIAHE
                     INPAIKVGCMVIAMPTYPLTPNPADVLAVMNADHENLAFSDVHVRGEYPGYFLRTLRD
                     AGIVLDITDEDRETLKNTVDFVSFSYYMSVCQAAGGGEVGEGNILGGIRNPTLPASEW
                     GWQIDPVGLRIVLNQFWDRWHKPLFIVENGLGAKDVLVEVDGVQTVEDDYRIAYLNDH
                     LVQVREAIADGVNLLGYTWWGPIDVVSASTAQLSKRYGFIYVDRNDDGSGTLNRYRKK
                     SFGW"
     gene            complement(3624..>4426)
                     /locus_tag="PLF56_08385"
     CDS             complement(3624..>4426)
                     /locus_tag="PLF56_08385"
                     /inference="COORDINATES: protein motif:HMM:NF012578.2"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_process: GO:0009401 - phosphoenolpyruvate-dependent
                     sugar phosphotransferase system [Evidence IEA]"
                     /codon_start=3
                     /transl_table=11
                     /product="glucose PTS transporter subunit IIA"
                     /protein_id="HOQ53617.1"
                     /translation="KLAGPSALSGWLAGITEPVIYGVNLPRKLPFYFGIAGGAVGGIF
                     AGIDGGRTTAFVFPSLIGIPAYLATPNLALLFIGVAIAVALAFFLTLFWGVKDDQVAG
                     EPATAAKVGAPAGTEVLVLAPMDGDLIALADVPDPVFSSGAMGTGVGVVPTSGTVYAP
                     VSGTVIVTMDSGHAYGIRTADGVEVLVHVGIDTVNLKGEGFTPKVHQGESVTAGDVLA
                     EVDLDAVKAAGYSTTTVVLITNSAAQSEVLPVDPSIVSHADTALVVTH"
BASE COUNT          722 a         1429 c         1457 g          718 t
ORIGIN      
        1 ggcaccagtc cgaactgcgc caccagcacc gccgcgatcg gtgcgaacag ggccgagcag
       61 tcggcggcca ccttgaccag cgagtacacc ttggtgagtt cggcgcgggg aacgtcctcg
      121 accagcaggc agtcccagga gttctgggtc acctgccagg cgccattgat caccgaggcc
      181 accaggaacc accagaagtt ctgcgcgaag gcccagatca ggcacgggat cacccaggcg
      241 acgatgtcga acgctgcggt ggtcatccga cggccgagct tgtcggtgat caccccgctg
      301 agtaggccga acaccacctg ggacagcatc gagatggtcg ccaacagccc gatctgctgg
      361 tcgcgcaccc cgagggccag catgaacacc gaggcgtagg gcagcaccag cgccatcgag
      421 aggccccaca tcggctcggt gtagacgcag gcccgtccgt tgccgcgaag tccgagcagg
      481 ctgaccacga gagggttgcg aacgcggggc acctgaccag tcaatcaccc ggatcgaacc
      541 cgcgctgggt catggtgtgc agcgcccacc cgccgtcccg gtgtcgccgc gttcccggct
      601 gcaactgcgc ggcacgaaca ctgccgatgg accgcggcta ccgtgccagc cggcgccagc
      661 cgccggcgcg attgttggcg atcaccgcac ggaacagggc cagcagggca gcctcgtcca
      721 gctcgtcccc ctcgaagagc tgaatggagc gtgcggtcgc gttgcctcgc ccctggttga
      781 tcagcccggt cggatcgggt gcgatcgggt cgtagatgaa gacgttgaga tggtccttgg
      841 tgccgaggaa cgcggccacg ttgccctgca gcacgaagta gggctggcgc gtgcgtttga
      901 tcgtctcttc gagttccggg tcggcctcgt gcagcagcac ccggacgcgg cgggcgaagt
      961 cggcctgcca cgccggcatc gtggcgagat agtcgtccac tcgtgggtcg gtggtggtca
     1021 tccgtcctcc gctcccggcg ccgcacctgc gcgcgttcag ggtcgagtct gaccaagtga
     1081 gcgagcgcgt tccacccttt gccccaatga gatcccggct gggcgccggg atgacgggtt
     1141 ggcctgctcc aatgagatcc cggcgtgcgc cgggatgacg ggttggcctg ctccaatgag
     1201 atccctgcgt gcgccgcgac aacgggttgg cggtggtctc gaccgttcgg taccggcact
     1261 cgtgaccgac acccgagcga cggtcacacc cgggtgcgca tgccctgttc catgcgggcg
     1321 acgtgcatgg tgaggtaggt gacctcgtcc tcgctgaccg gatgctgcaa acgcaactcc
     1381 aggagcgact tgatgcggag cgccacctgt tgtgcccgcc ccttgccgga cgcgacgagc
     1441 accgccacgt cgggtatgcc tccgtccagt tgcctgccgg cctgggcgcg ggcgaagaag
     1501 tagcgcaggt gggtggcgaa gcgggccgcg tcgatcgagt cggggtcgaa ctcgtcgccg
     1561 taggcctgct gcaccgtgtc gaagatctgc cggatcagct tcgactgcac gaaggtctgc
     1621 tccatgctcg gactgccggt gaccgcgtgg aacaggtgca tggcgagggc gatcgcctcg
     1681 ccctgcggca gcggcgcgtc cagctcgcgg ttcagcttgg ccagcaacgc ctcggccacc
     1741 tcgagctcga ccgggtgcag atgggccacc tcggcccgca tcgggtactc catcacttca
     1801 ccgcgggcca cccgttcaat cgcctggtgg acgtggtcgg ccaccgccac cacagccagc
     1861 ggcggcatca gcgagccgag ctcgcgcatc gcctgcgcga acagggtctc gaccagcgcc
     1921 agccgctccg gtggaatgtc ggccagtacc tgcgcgatcg agtcgggatt gtcggccggg
     1981 acgaaccgtc gcacgatcag cgccgggtcg atggggtcac cggggcgggc ctggaagccc
     2041 acgccgcgcc cggtgatcac cacctccgga ccgagttcgt cgagcgagag cacgacgttg
     2101 ttgttgaaca ccttcagcac ctgcatcgca cnnnnnnnnn nnnnnnnnnn nnnnnnnnnn
     2161 nnnnnnnnnn nnnnnnnnnn nnnnnnnnnn nnnnnnnnnn nnnnnnnnnn nnnnnnnnnn
     2221 nnnnnnnnnn ntaccagccg aacgacttct tgcggtacct gttcagggtg cccgacccgt
     2281 cgtcgttgcg gtcgacgtag atgaacccgt accgcttcga cagctgcgcg gtggacgcgc
     2341 tgaccacgtc gatcgggccc caccaggtgt agccgagcag gttcactccg tcggcgatcg
     2401 cctcgcgcac ctgcaccagg tgatcgttga ggtaggcgat ccggtagtcg tcttcgacgg
     2461 tctgcacgcc gtcgacctcg accagcacgt ccttggcgcc cagcccgttc tcgacgatga
     2521 acagcggctt gtgccagcgg tcccagaact ggttcagcac gatgcgcagg ccgaccggat
     2581 cgatctgcca accccattcg gacgccggca gggtcgggtt gcggatgccg cccagaatat
     2641 tgccctcgcc gacctcgcca ccgcccgcgg cctggcagac gctcatgtag tagctgaacg
     2701 acacgaagtc gacggtgttc ttcagcgtct cgcggtcttc gtcggtgatg tcgagcacga
     2761 tgcccgcatc gcgaagcgtg cgcaggaagt acccggggta ttcgccgcgc acgtgcacgt
     2821 cgctgaacgc gaggttctcg tggtcggcat tcatcaccgc gagcacgtcg gccggattcg
     2881 gcgtcagcgg ataggtcggc atggcgatca ccatgcagcc caccttgatc gccgggttga
     2941 tctcgtgcgc gatcttcgtg gcggacgccg acgcgaccaa ctcgtggtgg atcgcctggt
     3001 acaggtcggc ctcgctgagc tcggccttcg gcgtccagat gccgccggac atcagcggcg
     3061 catgcagcac cgagttgatc tcattgaagg tcagccagta cttcacccgg tcaccgaacc
     3121 gctcgaacag cacccgtgcg taacgctcgt agaagccgat cagccggcga tcgacccagc
     3181 cgtcgtaggt cttggccagg tgcagcggcg tctcgtagtg gctgatcgtg accagcggtt
     3241 cgatgccgta cttctccaac tcgtccagca gccgatcgta gaaggccagg ccctcctcgt
     3301 tcggcgtcag ttcgtcgccg ttgggaaaga tccggctcca ggcgatcgag aagcggtaga
     3361 ccttgaagcc catctcggcg aacagcgcga tgtcttcggc gtagcggtga tagtggtcga
     3421 tgccttcgtg cttgaggttg tcgggggtcg gttcgtccgt gggcggggcg acgatgccgc
     3481 gcggcatcac gtcctggatc gaaaggccct tgccatcggc gtcgtaagcg ccttcgagtt
     3541 gattggcggc ggtggcgcca ccccacagaa agccgtctgg aaatgtggtg gtcatgtgag
     3601 tcattctcct gtctcggtgg tgttcagtgg gtgacgacga gcgctgtgtc ggcgtggctg
     3661 acgatggacg ggtcgaccgg cagcacctcg ctttgggcgg cactgttggt gatcaagacc
     3721 accgtggtgg tcgagtagcc ggcggccttg accgcgtcga gatcgacctc ggcgagcacg
     3781 tcgccggcgg tgaccgactc accctgatgc accttggggg tgaagccctc ccccttcagg
     3841 ttcaccgtgt cgatgccgac gtgcacgagc acctcgacgc cgtccgcggt gcgaatgccg
     3901 taggcgtgac ccgaatccat ggtgacgatc acggtgccgc tgaccggcgc atagacggtt
     3961 ccggacgtcg ggacgacccc cacgccggtg cccatcgcac cactggagaa caccggatcg
     4021 ggcacgtccg ccagggcgat caggtcgccg tccatcggtg cgagaaccag cacctccgta
     4081 ccggcaggag cgcccacctt cgctgcggtg gcgggttcgc cggccacctg gtcgtccttg
     4141 acgccccaga acagggtgag gaagaaggcc agcgccacgg cgatcgcgac accgatgaac
     4201 agcagcgcca ggttcggagt ggcgaggtag gcgggaatgc cgatcagcga gggaaagacg
     4261 aacgcggtgg ttcgaccacc gtcgatgccg gcgaagatgc caccgaccgc accgccggcg
     4321 atgccgaagt agaacggcag tttgcgaggc aggttgacgc cgtagatgac gggctcggtg
     4381 atgccggcca accagcccga cagcgcggac ggtccggcca gcttct
//
LOCUS       DAOSUG010000232         4423 bp    DNA     linear   ENV 10-JUN-2024
DEFINITION  MAG TPA_asm: Micropruina sp. isolate AMR_MDS_2195
            NODE_8452_length_4423_cov_3.852564, whole genome shotgun sequence.
ACCESSION   DAOSUG010000232 DAOSUG010000000
VERSION     DAOSUG010000232.1
DBLINK      BioProject: PRJNA925477
            BioSample: SAMN32864262
            Sequence Read Archive: SRR4236673
KEYWORDS    WGS; ENV; Metagenome Assembled Genome; MAG; Third Party Data; TPA;
            TPA:assembly.
SOURCE      Micropruina sp. (wastewater metagenome)
  ORGANISM  Micropruina sp.
            Bacteria; Bacillati; Actinomycetota; Actinomycetes;
            Propionibacteriales; Nocardioidaceae; Micropruina.
REFERENCE   1  (bases 1 to 4423)
  AUTHORS   Abdulkadir,N., Saraiva,J.P., Zhang,J., Stolte,S., Gillor,O.,
            Harms,H. and Rocha,U.
  TITLE     Genome-centric analyses of 165 metagenomes show that mobile genetic
            elements are crucial for the transmission of antimicrobial
            resistance genes to pathogens in activated sludge and wastewater
  JOURNAL   Microbiol Spectr 12 (3), e0291823 (2024)
   PUBMED   38289113
REFERENCE   2  (bases 1 to 4423)
  AUTHORS   Abdulkadir,N.
  TITLE     Direct Submission
  JOURNAL   Submitted (24-MAR-2023) Environmental Microbiology, Helmholtz
            Center for Environmental Research -UFZ, Permoserstr 15, Leipzig
            04318, Germany
COMMENT     The annotation was added by the NCBI Prokaryotic Genome Annotation
            Pipeline (PGAP). Information about PGAP can be found here:
            https://www.ncbi.nlm.nih.gov/genome/annotation_prok/
            
            ##Genome-Assembly-Data-START##
            Assembly Method        :: SPAdes v. 3.11.1
            Genome Representation  :: Full
            Expected Final Version :: Yes
            Genome Coverage        :: Not Applicable
            Sequencing Technology  :: Illumina MiSeq
            ##Genome-Assembly-Data-END##
            
            ##Genome-Annotation-Data-START##
            Annotation Provider               :: NCBI
            Annotation Date                   :: 05/04/2023 04:57:10
            Annotation Pipeline               :: NCBI Prokaryotic Genome
                                                 Annotation Pipeline (PGAP)
            Annotation Method                 :: Best-placed reference protein
                                                 set; GeneMarkS-2+
            Annotation Software revision      :: 6.5
            Features Annotated                :: Gene; CDS; rRNA; tRNA; ncRNA
            Genes (total)                     :: 2,776
            CDSs (total)                      :: 2,750
            Genes (coding)                    :: 2,714
            CDSs (with protein)               :: 2,714
            Genes (RNA)                       :: 26
            tRNAs                             :: 24
            ncRNAs                            :: 2
            Pseudo Genes (total)              :: 36
            CDSs (without protein)            :: 36
            Pseudo Genes (ambiguous residues) :: 0 of 36
            Pseudo Genes (frameshifted)       :: 2 of 36
            Pseudo Genes (incomplete)         :: 32 of 36
            Pseudo Genes (internal stop)      :: 2 of 36
            ##Genome-Annotation-Data-END##
FEATURES             Location/Qualifiers
     source          1..4423
                     /organism="Micropruina sp."
                     /mol_type="genomic DNA"
                     /submitter_seqid="NODE_8452_length_4423_cov_3.852564"
                     /isolate="AMR_MDS_2195"
                     /isolation_source="Activated sludge of municipal
                     wastewater treatment plants"
                     /db_xref="taxon:2737536"
                     /environmental_sample
                     /geo_loc_name="Canada"
                     /lat_lon="49.25 N 123.1 W"
                     /collection_date="2015-06-16"
                     /metagenome_source="wastewater metagenome"
                     /note="metagenomic"
     gene            complement(<1..244)
                     /locus_tag="PLF56_08390"
     CDS             complement(<1..244)
                     /locus_tag="PLF56_08390"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_017934883.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_function: GO:0005524 - ATP binding [Evidence IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="UvrD-helicase domain-containing protein"
                     /protein_id="HOQ53618.1"
                     /translation="MSTLTAPEQLSELLGLPFSDQQLAAITAPLQPGVIIAGAGSGKT
                     TVMAARVVWLVGSGQVAPEQVLGLTFTRKAAAELSAR"
     gene            complement(241..3441)
                     /locus_tag="PLF56_08395"
     CDS             complement(241..3441)
                     /locus_tag="PLF56_08395"
                     /EC_number="3.6.4.-"
                     /inference="COORDINATES: protein motif:HMM:NF012789.2"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_function: GO:0003677 - DNA binding [Evidence IEA];
                     GO_function: GO:0004386 - helicase activity [Evidence
                     IEA];
                     GO_function: GO:0004519 - endonuclease activity [Evidence
                     IEA];
                     GO_function: GO:0005524 - ATP binding [Evidence IEA];
                     GO_function: GO:0016887 - ATP hydrolysis activity
                     [Evidence IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="ATP-dependent DNA helicase"
                     /protein_id="HOQ53619.1"
                     /translation="MDEVADMPDAWGLDAWQRAAVHEHRHSPALVIGGPGSGKTTVVA
                     AAAALELGTRAGDGPQPLVLTFSRRSASRLRNRVARAMNRTVSPPWVTTTHAFCLWLL
                     RLAAEPGQPEPRLLTAPEQEFRVRELLTGRGAGVWPPEVQQAIGTRAFARQARAAIAR
                     VRQLGLDPEDVVAFAAAAGRPEWVTTGHFFAEYLDVLDAEGSLDYAELVHRARIALTD
                     PQLVQSVRARISRVIVDEYGELDPSQIGLLRALVPPGGPILAVGDPDQVVFGFRGAHP
                     RALAQFSSTFPLADGSPAPVLVLPANHRSGAVVAVAARRVARRLPLPVLGEQVVAALR
                     PADEPETVVPEVSADEVAVMVCASESQQAQLIAEQLRRAHLDEGYAHGDLAVLVRSGR
                     RQLAPIARALVAAGIPVEVAGDEIPLLAEPAVRPLLLALEVVLRGGELDADEAKRLLT
                     SPLARVDAVGLRTLGRLLLETEREATGSAMVGRSPGQLIAEVLSEPERLADLPETAEV
                     GALRALSGLLQAARGTLAVPGNGASEVLWRLWSGTDWGERLRRASRDGGEAGRRADRD
                     LDAVIALFAAAAATEQPAGSGGVRGFLAEVAAQEIPADTTREAEVRGRGVRLLTAHRA
                     KGLQWPVVVVAGVQEGLWPAPGHGAAILEPAELVSDGLSGHSDPRQALTDERRLFHVA
                     CSRAARRLVVTAVQGSDGEANQPSRFLAELGVPIVEYRPSERPLTLAALVADLRRRAT
                     SADCSAALREAAALRLARLAALTDARGRPLVAQAHPSLWWGLAEPTGAARPAALPVRL
                     SPSALTGLLTCPRQHYLTRQVHADPPRNANASLGSVIHVLAEHARNDDLSIDELAGRL
                     DEVWAELPFDAAWLSDAERIKAESALTRFVNWQAANEGAEVVGVEVPFDVTVEVDGTP
                     VRLVGSVDRLERAADGRLRVVDFKTNKRPPTAAEAASHEQLGVYQLAIEAGGFDSVAG
                     AGARSAGGCLVYLRLPESADLDDYPRQFHQAALSERPSLGEADAGHPSWVHERVSRAV
                     TLVREGRYPATPGQGCQWCPFAASCPAKPSGRQVVA"
     gene            3635..3838
                     /locus_tag="PLF56_08400"
     CDS             3635..3838
                     /locus_tag="PLF56_08400"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_010850467.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="cold shock domain-containing protein"
                     /protein_id="HOQ53620.1"
                     /translation="MAEGTVKWFSAEKGYGFIEVDGGTQDVFVHQTAIHSDGFRTLSE
                     GQRVEFEITSGAKGPQADSVRGL"
     gene            4008..>4423
                     /locus_tag="PLF56_08405"
     CDS             4008..>4423
                     /locus_tag="PLF56_08405"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_016669201.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="toxic anion resistance protein"
                     /protein_id="HOQ53621.1"
                     /translation="MSSTTPAAGAAAAMPLQPPTTLTLTVPEAPSAVVATQAPAMAPQ
                     VEAAQVPVLDAKVDEFLGALDSAEAKSPTFTAQADAVRSMGDADIRKAAETSNRLLNA
                     PVRALKEGGLSDGSKVGQTLVQLRRTVEDLDPGKARG"
BASE COUNT          656 a         1683 c         1527 g          557 t
ORIGIN      
        1 cccgggcgga cagctcggcc gcggccttgc gggtgaaggt caggcccagc acctgctcgg
       61 gggctacctg gccgctgccg accagccaca ccacccgggc cgccatcacg gtggtcttgc
      121 ccgaaccggc accggcgatg atcacaccgg gctgcaacgg agcggtgatc gccgcgagtt
      181 gctgatctga gaacggcagc ccgagcagtt cgctcagctg ttcgggtgcg gtgagcgtgc
      241 tcacgcgacc acctgccgtc cggacggctt cgcggggcag ctcgcagcga acgggcacca
      301 ctggcaaccc tgccccggcg tggccgggta ccggccctca cgcaccaggg tgaccgcgcg
      361 ggacactcgc tcgtgcaccc agctgggatg cccggcgtcc gcctcaccca gactcggacg
      421 ttcgctcagc gccgcctggt ggaactgacg cggatagtcg tcgagatcgg cgctctcggg
      481 caggcgcagg tagaccaggc agccaccggc gctgcgcgcc ccggcacccg ccacggagtc
      541 gaacccaccg gcctcgatgg ccagctgata caccccgagt tgctcgtgcg aggcggcctc
      601 ggccgcggtg ggcggacgct tgttggtctt gaagtcgacc acccgcagcc ggccgtcggc
      661 ggcccgctcc agccggtcga cgctacccac caggcgcacc ggggtgccgt ccacctcgac
      721 ggtgacgtcg aacggcacct cgacgcccac cacctcggca ccctcgttgg cggcctgcca
      781 gttgacgaag cgggtcagcg ccgattcggc cttgatgcgc tcggcgtccg agagccacgc
      841 cgcatcgaac ggcagctcgg cccagacctc gtcgagccgg ccggccagct cgtcgatgct
      901 caggtcgtcg ttgcgtgcgt gttcggccag cacgtggatc accgagccca ggctggcgtt
      961 cgcgttgcgc ggcgggtcgg cgtgcacctg ccgggtgagg tagtgctgcc ggggacacgt
     1021 gagcaacccc gtcaaggcgg acgggctgag ccgcacgggc aacgccgccg gacgcgcggc
     1081 gccggtcggt tcggccaggc cccaccacaa cgagggatgc gcctgcgcca ccaacggacg
     1141 cccgcgggcg tcggtgagcg ccgccagccg ggccagccgc agcgccgccg cctcacgcaa
     1201 ggccgcggag cagtcggcgc tggtggcgcg tcggcgcaga tcggccacca gcgcagccag
     1261 ggtgagcgga cgttcgctgg gccggtactc gacgatcggc acccccagct cggccaggaa
     1321 gcgcgagggc tggttggcct cgccgtcgga gccctggacg gcggtcacca ccaggcggcg
     1381 agcggcccgg ctgcacgcca cgtggaacag cctgcgctca tcggtcagcg cctgccgcgg
     1441 gtcggagtgc ccgctcaacc cgtcgctgac caactcggcc ggctcgagaa tggctgcgcc
     1501 gtgcccgggt gccggccaca acccctcttg gacgcccgcc acgacgacca ccggccactg
     1561 caggcccttg gcccggtgcg cggtcaacag tcggacgccc cggccccgca cctcggcctc
     1621 acgggtggtg tcggccggaa tctcctgcgc cgccacttcg gccagaaagc cccggacgcc
     1681 gcccgacccc gccggctgtt cggtcgcagc ggccgccgcg aacagcgcga tcaccgcgtc
     1741 gaggtcacgg tcggcccgcc ggcccgcctc gccgccgtcg cggctggcgc gccgcagccg
     1801 ctcgccccag tcggtgccgg accacagccg ccacaacacc tcggacgccc cgttgccggg
     1861 cacggccagg gtgccccgag cggcttggag cagccccgac aacgcccgca gcgcccccac
     1921 ctcggcagtc tcgggcaggt cggccagccg ttcgggttcg ctcaacacct cagcgatcag
     1981 ctgccccggc gagcggccga ccatggccga accggtggcc tcgcgctcgg tctcgagcag
     2041 cagccggccc agggtgcgca acccgaccgc gtcgacccgg gccaatggtg aggtgagcag
     2101 ccgtttggcc tcgtcggcgt cgagctcgcc gccgcgcagc accacctcga gggccagcag
     2161 cagtggacgc accgccggtt cggccagcag cggaatctcg tcgccggcca cctcgaccgg
     2221 aatgcccgcc gccaccagcg cccgggcgat cggggcgagc tggcgccgtc cggagcgcac
     2281 cagcacggcc aggtcgccgt gggcatagcc ctcgtcgagg tgggcgcggc gcagctgttc
     2341 ggcgatcagc tgcgcctgct gcgactccga cgcacacacc atgaccgcga cctcatcggc
     2401 gctcacctcg ggcaccaccg tctcgggctc gtccgccggt cgcaacgcgg cgacgacctg
     2461 ttcgccgagc accggtagcg gcagccggcg cgccacccgc cgcgcggcca ccgcgacgac
     2521 ggcacccgag cgatggttgg cgggcagcac cagcaccggc gccggtgacc cgtcggccag
     2581 ggggaacgtg gacgagaact gggccagtgc ccgcggatgt gcgccacgga acccgaacac
     2641 cacctgatcg ggatcgccca ccgcaaggat cggaccgccg ggcggcacca acgcccgcag
     2701 cagcccgatc tgcgaggggt cgagctcgcc gtactcgtcg acgatcaccc gcgagatgcg
     2761 cgcacgcacc gactgcacca gctgcgggtc ggtcagcgcg atgcgggccc ggtggacgag
     2821 ttcggcgtag tcaagactgc cttcggcatc gagcacatcg aggtactcgg cgaagaaatg
     2881 cccggtggtc acccactcgg gccgcccggc cgccgccgcg aacgcgacca cgtcttcggg
     2941 atcgagcccc agttgccgga ccctggcgat ggccgcccgc gcctgtcggg cgaaggccct
     3001 ggtgccgatg gcctgctgaa cctctggcgg ccagacgccc gccccccgtc cggtgagcag
     3061 ctcgcgcacc cggaactcct gctcgggcgc ggtcagcagc cgtggctcgg gctgccccgg
     3121 ctcggccgcc aggcgcaaca accacaagca gaaggcgtgc gtggtggtga cccacggcgg
     3181 gctgaccgtg cggttcattg cacgagcgac ccgattgcgc aaccggctgg ccgagcgtcg
     3241 agagaaggtg agcaccagtg gttgcggccc gtcgcccgcc cgcgtgccga gttcgagcgc
     3301 cgccgccgcg gccaccaccg tggtcttgcc cgagccgggc ccgccgatca ccagggccgg
     3361 cgaatgccgg tgctcatgca cggcggcgcg ctgccacgca tcgagccccc acgcatcagg
     3421 catgtcagcg acctcgtcca tgcccacatg caatcatccg gcaccgacat ttcccgcttg
     3481 ccgacacgcg caacgcgttg tccgcgtgag tgacatgggt cgtgagcatg tgacagggtg
     3541 actgaaccgc tgcgatgagg tgcacgctcg ggcggcggtc caccagcacg agtcaccgcc
     3601 cctggtgagg gagtgcagcc agaaggagaa ccgcatggcc gagggcaccg tgaagtggtt
     3661 cagtgccgaa aagggatacg gcttcatcga ggtcgacggt ggcacccaag acgtcttcgt
     3721 ccatcaaacg gcgatccatt ccgacggttt ccgcaccctt tccgagggtc agcgggtcga
     3781 gttcgagatc acgtccggcg ccaagggccc gcaggccgac agcgtcaggg ggctgtgagc
     3841 ccgcgcggtt ccatggccga gccgacccca gggggcgctc cactccgggc gtggcccgtc
     3901 atcagcgggt gaccttccag ggttcttccc cacccgtggc tcatgcctgc cgcccacccg
     3961 accgctagat tgggcggcat accctgttca cccatcgaga ggtagtcatg agctcgacca
     4021 ctcccgccgc cggtgccgcc gcggccatgc cgctgcagcc tccgaccacc ctcaccctga
     4081 ccgtgcccga ggctccctcg gccgtggtgg ccacgcaggc cccggccatg gcaccgcagg
     4141 tcgaggccgc gcaggtgccg gtgctcgacg ccaaggtgga cgagtttctg ggggctctcg
     4201 attcggccga ggccaagtcg cccacgttca ccgcgcaggc cgacgccgtg cgcagcatgg
     4261 gcgacgccga catccgcaag gccgccgaga cctccaaccg cctgctgaac gcgccggtcc
     4321 gcgccctgaa ggagggtggc ctgtccgacg gatccaaggt cggccagacc ctggtgcagt
     4381 tgcgccgcac cgttgaggat ctcgatccgg gcaaggcgcg cgg
//
LOCUS       DAOSUG010000233         4409 bp    DNA     linear   ENV 10-JUN-2024
DEFINITION  MAG TPA_asm: Micropruina sp. isolate AMR_MDS_2195
            NODE_8489_length_4409_cov_4.211300, whole genome shotgun sequence.
ACCESSION   DAOSUG010000233 DAOSUG010000000
VERSION     DAOSUG010000233.1
DBLINK      BioProject: PRJNA925477
            BioSample: SAMN32864262
            Sequence Read Archive: SRR4236673
KEYWORDS    WGS; ENV; Metagenome Assembled Genome; MAG; Third Party Data; TPA;
            TPA:assembly.
SOURCE      Micropruina sp. (wastewater metagenome)
  ORGANISM  Micropruina sp.
            Bacteria; Bacillati; Actinomycetota; Actinomycetes;
            Propionibacteriales; Nocardioidaceae; Micropruina.
REFERENCE   1  (bases 1 to 4409)
  AUTHORS   Abdulkadir,N., Saraiva,J.P., Zhang,J., Stolte,S., Gillor,O.,
            Harms,H. and Rocha,U.
  TITLE     Genome-centric analyses of 165 metagenomes show that mobile genetic
            elements are crucial for the transmission of antimicrobial
            resistance genes to pathogens in activated sludge and wastewater
  JOURNAL   Microbiol Spectr 12 (3), e0291823 (2024)
   PUBMED   38289113
REFERENCE   2  (bases 1 to 4409)
  AUTHORS   Abdulkadir,N.
  TITLE     Direct Submission
  JOURNAL   Submitted (24-MAR-2023) Environmental Microbiology, Helmholtz
            Center for Environmental Research -UFZ, Permoserstr 15, Leipzig
            04318, Germany
COMMENT     The annotation was added by the NCBI Prokaryotic Genome Annotation
            Pipeline (PGAP). Information about PGAP can be found here:
            https://www.ncbi.nlm.nih.gov/genome/annotation_prok/
            
            ##Genome-Assembly-Data-START##
            Assembly Method        :: SPAdes v. 3.11.1
            Genome Representation  :: Full
            Expected Final Version :: Yes
            Genome Coverage        :: Not Applicable
            Sequencing Technology  :: Illumina MiSeq
            ##Genome-Assembly-Data-END##
            
            ##Genome-Annotation-Data-START##
            Annotation Provider               :: NCBI
            Annotation Date                   :: 05/04/2023 04:57:10
            Annotation Pipeline               :: NCBI Prokaryotic Genome
                                                 Annotation Pipeline (PGAP)
            Annotation Method                 :: Best-placed reference protein
                                                 set; GeneMarkS-2+
            Annotation Software revision      :: 6.5
            Features Annotated                :: Gene; CDS; rRNA; tRNA; ncRNA
            Genes (total)                     :: 2,776
            CDSs (total)                      :: 2,750
            Genes (coding)                    :: 2,714
            CDSs (with protein)               :: 2,714
            Genes (RNA)                       :: 26
            tRNAs                             :: 24
            ncRNAs                            :: 2
            Pseudo Genes (total)              :: 36
            CDSs (without protein)            :: 36
            Pseudo Genes (ambiguous residues) :: 0 of 36
            Pseudo Genes (frameshifted)       :: 2 of 36
            Pseudo Genes (incomplete)         :: 32 of 36
            Pseudo Genes (internal stop)      :: 2 of 36
            ##Genome-Annotation-Data-END##
FEATURES             Location/Qualifiers
     source          1..4409
                     /organism="Micropruina sp."
                     /mol_type="genomic DNA"
                     /submitter_seqid="NODE_8489_length_4409_cov_4.211300"
                     /isolate="AMR_MDS_2195"
                     /isolation_source="Activated sludge of municipal
                     wastewater treatment plants"
                     /db_xref="taxon:2737536"
                     /environmental_sample
                     /geo_loc_name="Canada"
                     /lat_lon="49.25 N 123.1 W"
                     /collection_date="2015-06-16"
                     /metagenome_source="wastewater metagenome"
                     /note="metagenomic"
     gene            <1..1146
                     /locus_tag="PLF56_08410"
     CDS             <1..1146
                     /locus_tag="PLF56_08410"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_013861547.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="DUF3372 domain-containing protein"
                     /protein_id="HOQ53622.1"
                     /translation="GVDGSQVTIYGEGWNFGEVAHDARFVQARQGNLAGTGIGTFNDR
                     LRDAVRGGGPFDEDPRGQGFGTGLFTASNDAWVNGDGYAQLDRLLADTDLIQLGLIGN
                     LRDFWLPSNSRRAFVRGDELDYNGQPAGYAAEPDEVVNYVDAHDNETLFDALTLKLRP
                     DTSMADRVRMNTLCLALATLGQAPVMWHAGTDILRSKSLDRNSYNSGDWFNFLDWTMT
                     DNGFGAGLPPEGDNGPKWRYFVPFLADSRFKPCPDDIAQAHAQALDLLRLRAATRLFR
                     MASGHAIRTKITFPVSNTHHQVPGVIAMRIDDRVGEPVQADWASLVAVFNATALPVRL
                     QVPGLTSRHGLHPVQAAGCDEVVKSARVEGQWAAVPARSYAVFVEPN"
     gene            1477..2163
                     /locus_tag="PLF56_08415"
     CDS             1477..2163
                     /locus_tag="PLF56_08415"
                     /inference="COORDINATES: protein motif:HMM:NF024701.2"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_function: GO:0003677 - DNA binding [Evidence IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="TetR family transcriptional regulator"
                     /protein_id="HOQ53623.1"
                     /translation="MATRSERRDNTESAIIDAGLQLLAEGGAEALTIRGLARKLVLVP
                     SALYRYVRNRDDLLNLLLTHARADLIEAVQAAHDAVPHHDLSGRWRAFARSLRSWSLA
                     HRHEWLLIQRTALTGTAPATENTYALHVLLLRLGADAEAAGMAPALLPRGTSAGMPGL
                     PVMLAMAGVHVSEQTVLAGLAAWHLLDGSIYAEVLSLAGRELIDADTYFDAMVAVTTR
                     MLVGQQGEGL"
     gene            complement(2220..2963)
                     /locus_tag="PLF56_08420"
     CDS             complement(2220..2963)
                     /locus_tag="PLF56_08420"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_011753909.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_component: GO:0016020 - membrane [Evidence IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="YitT family protein"
                     /protein_id="HOQ53624.1"
                     /translation="MTAAGGTGTNTGPTTPDANPFVKNARPGSPAPTAPRPTTPAPIT
                     PAPRLTAARHTLVEDILGFCTGTVMASFGLYLLHASATVTGGTAGLSLLLSYALPVPF
                     GVLFLLVNLPFLVAAVSRKGWTFTLRTLLAIGAVTVLTSLHAAMLPHLVLPAVYSVVL
                     GNMIAGVGLLVLFRHRASLGGFNIVGLLLQERRGIPAGYVQMVLDLMVVLGSLAVLPW
                     PSVAFSALGAVILNLVIAFNHRPDRYVGW"
     gene            complement(2960..4042)
                     /gene="hisC"
                     /locus_tag="PLF56_08425"
     CDS             complement(2960..4042)
                     /gene="hisC"
                     /locus_tag="PLF56_08425"
                     /EC_number="2.6.1.9"
                     /inference="COORDINATES: protein motif:HMM:NF002878.0"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_function: GO:0004400 - histidinol-phosphate
                     transaminase activity [Evidence IEA];
                     GO_process: GO:0000105 - histidine biosynthetic process
                     [Evidence IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="histidinol-phosphate transaminase"
                     /protein_id="HOQ53625.1"
                     /translation="MTTAIRTPHIRSAVTSLPPYVAGRRATSNATASLASNENHFPPL
                     PSVLDEIAWASGRINRYPDSAAEALRERIAAHVGVRSDEVAAGPGSVGVLQQIIASVC
                     DAGDEVVFAWRSFEAYPLLVTLSGAKPVPVPLTRHETHDLDAMAAAITPRTRLVLLCT
                     PNNPTGVTIAGADLDRLLARVPGDVLVAIDEAYLEYVTTDPLDALARYRRYPNVCLLR
                     TFSKAYGLAGLRVGYAIASPTVAGALRRTGLPFAVSSLAQAAAIASLDASRELAERVA
                     AVATERDRVTCAMRRLGLAVPGSQANFVWLRTGEGEREAVLRAFDAADILVRGYAGDG
                     VRITIADAASNDRVLQALAELQRAAA"
     gene            4143..>4409
                     /locus_tag="PLF56_08430"
     CDS             4143..>4409
                     /locus_tag="PLF56_08430"
                     /inference="COORDINATES: protein motif:HMM:NF024212.2"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="winged helix-turn-helix transcriptional
                     regulator"
                     /protein_id="HOQ53626.1"
                     /translation="MADVDPLDARILLALDDEPDASVLQLARTLAISRNTVHARLQRM
                     LREGALLGQSQRIAPASLGYPVVGFVSLSVSQARGEGTAAAMAEV"
BASE COUNT          720 a         1477 c         1548 g          664 t
ORIGIN      
        1 ggggtggacg gatcgcaggt cacgatctac ggcgagggct ggaacttcgg cgaggtggcc
       61 cacgacgcgc ggttcgtcca agccaggcag ggcaacctgg ccggaaccgg tatcggcacg
      121 ttcaacgacc ggctgcgcga tgccgtccgg ggcgggggac cgttcgacga ggatccgcgc
      181 ggccagggct tcggcaccgg gctgttcacg gcatcgaacg acgcgtgggt gaacggggac
      241 ggctacgcgc aactcgatcg gctactggcc gacaccgacc tgatccaact cggcctgatc
      301 ggcaacctgc gcgacttctg gctgccctcc aacagcaggc gggcgttcgt ccggggcgac
      361 gaactggact acaacggcca gccggcgggt tacgcggccg agccggacga ggtggtcaac
      421 tacgtggacg cgcacgacaa cgagaccctg ttcgacgcgc tcaccctgaa gctgcggccc
      481 gacacgtcga tggccgaccg ggtgcgcatg aacacgctgt gcctggccct ggcgaccctg
      541 gggcaggccc cggtgatgtg gcacgccggc accgacattc tgcgcagcaa gagcctggat
      601 cgaaactcgt acaactccgg cgactggttc aacttcctcg actggacgat gaccgacaac
      661 ggcttcggcg ccggcttgcc gcccgagggc gacaacgggc cgaagtggcg ctacttcgtg
      721 ccctttctgg ccgattcgcg gttcaagccc tgcccggacg acatcgccca ggctcacgcg
      781 caggcgctcg acctgcttcg gttgcgcgct gccacccggt tgttccgcat ggcctcgggt
      841 cacgcgatcc gcaccaagat cacgtttccg gtcagcaaca cccaccacca ggtgcccggc
      901 gtgatcgcga tgcgcatcga cgatcgggtg ggtgagccgg tgcaggcgga ctgggcgtca
      961 ctggtcgcgg tgttcaacgc gaccgcgctg ccggtgcggc ttcaggtacc ggggctgacc
     1021 agccggcatg gcctgcaccc ggtacaggcc gccggctgcg acgaggtggt caagagtgcc
     1081 cgcgtcgagg ggcagtgggc ggccgtgccg gcccgcagct acgcggtctt cgtcgagccg
     1141 aactgagtct cttctcgacc ggacgcggga gaccagctga gaggtttctc gcgctgaact
     1201 gcgctgacgc aatgactgga tcggacggtc gcggccggtg ggccaggtgg gcggaggccc
     1261 gggttgcgtc ggtaaggccc gcaacccggg ccgggccacc aggtcgtcgt tttgaagaat
     1321 cacccgctca cggcataccc cccggatgcc gaggtccccc cggatcgtct gtgaacgacg
     1381 ttctctgaaa gcgcttcaat attgacaccc gccatgccag aatgcaaccg tccgggctgg
     1441 gcgaccaccc cttgaccaga tgaaatgacg tgagccatgg ccacccgctc cgagcggcgc
     1501 gacaacaccg agtcggcgat catcgacgcc ggcctgcagt tgctggccga gggcggcgcc
     1561 gaggcgctga cgatccgcgg tttggcccgc aagctcgttc tggtgccatc ggctctctac
     1621 cgctacgtgc gcaaccgcga cgatctgctc aatctgctgc tgacccacgc ccgtgcggat
     1681 ctgatcgagg ccgtgcaggc cgctcatgat gcggttcctc accacgacct atccggccgc
     1741 tggcgcgctt tcgcccgcag cctgcgctca tggtcgctcg cgcatcggca cgagtggttg
     1801 ctgattcagc gcaccgcgct gacgggcact gcgccggcca ccgagaacac ctacgccctc
     1861 cacgtgctgt tgcttcgcct cggtgccgac gccgaggcgg ccggcatggc gcccgcgcta
     1921 ctgcccagag gtaccagcgc aggtatgccg ggtctgcctg tcatgctcgc gatggccggt
     1981 gtgcacgtgt cggagcagac cgttctggcc ggacttgccg cctggcatct gctggacggc
     2041 tcgatctacg ccgaagtgct gagcttggcc ggccgggagt tgatcgatgc agacacgtac
     2101 ttcgacgcga tggtggcggt gacgacccgc atgctggtcg gccaacaagg tgaaggattg
     2161 tgattcgata tcggatcaca atccttcacc ttgttgacgg gttcatgggc ggcccggggt
     2221 caccacccca catagcggtc cggacggtgg ttgaaagcga tcaccaggtt gagtatcacc
     2281 gcgcccaggg ctgagaaggc cacgctgggc cagggcagca cggcgagcga gcccagcacc
     2341 accatcaggt cgagcaccat ctgcacgtag ccggccggaa tgccgcgccg ttcctgtagg
     2401 agcagtccga cgatgttgaa accgcccaga ctggcccgat gccgaaacag caccagcagc
     2461 ccgactccgg cgatcatgtt gcccagcacc acggaataga cggcgggcag caccaggtgc
     2521 ggcagcatgg cggcgtgcag cgaggtcagt accgtcacgg cgccgatcgc gagcagcgtc
     2581 cgtagggtga aagtccaccc cttgcgagaa accgccgcga ccaggaaggg caggttgacc
     2641 agcagaaaca gtaccccgaa cggcaccggc agggcgtaac tgagcagcag cgacagcccg
     2701 gctgtgccgc cggtgaccgt ggcgctggcg tgcagcagat acagcccgaa cgaggccatc
     2761 acggtgccgg tgcagaagcc caggatgtcc tccaccaggg tgtgccgcgc cgccgtgagc
     2821 cggggcgcgg gggtaatggg cgcgggcgtg gtgggccggg gagcggtggg cgcgggcgaa
     2881 ccaggccggg cattcttgac gaacgggttc gcgtcgggag tcgtcggccc cgtgttggtg
     2941 ccggtgccgc ccgccgccgt catgccgccg cccgctgcag ctcggccagc gcctgcagca
     3001 cccggtcgtt gctggcggcg tccgcgatgg tgatccgcac cccgtcgccg gcgtagccgc
     3061 gcaccagtat gtcggccgcg tcgaaagccc gcagcaccgc ctcgcgttcg ccctcgccgg
     3121 tgcgcagcca gacgaagttg gcctgcgagc cgggtaccgc cagccccaac cgtcgcatcg
     3181 cacacgtcac ccggtccctt tccgtggcga ccgccgccac ccgctcggcc agctcgcggg
     3241 aggcgtccag ggaggcgatc gccgcggcct gggcgaggct gctcaccgcg aacggcagcc
     3301 cggtgcggcg cagcgcaccg gcgacggtgg gggacgcgat ggcgtagccg acccgcagcc
     3361 cggccagccc gtaggccttc gagaaggtgc gcagcaggca cacgttgggg taccgccggt
     3421 agcgggcgag tgcgtccagg gggtcggtgg tcacgtactc gaggtacgcc tcatcgatcg
     3481 ccaccagcac gtcgccgggc acccgggcca gtaagcggtc gagatcagcg cccgcgatgg
     3541 tgaccccggt ggggttgttc ggcgtgcaca gcagcaccag ccgggtgcgg ggtgtgatcg
     3601 cggcggccat ggcgtccagg tcatgtgtct catgcctggt caggggtacc gggacgggtt
     3661 tcgccccgga cagcgtgacc aacagcgggt acgcctcgaa cgagcgccac gcgaacacca
     3721 cctcgtcccc ggcgtcgcac accgaggcga tgatctgctg cagcacgccg acgctgccgg
     3781 ggccggcggc gacctcgtcc gagcgcacgc cgacgtgcgc ggcgatccgt tcacgcaggg
     3841 cctcggcggc gctgtcgggg taccggttga ttcgtccgga cgcccacgcg atctcgtcca
     3901 gcaccgacgg tagtggcgga aagtggttct cgttcgaggc cagcgacgcg gttgcgttcg
     3961 aggtggcgcg gcgccccgcc acgtagggcg gcaatgaggt gacggccgaa cgaatgtgcg
     4021 gggtgcgaat cgctgtggtc atgaggggat gatgcgacgg ggcgaatggt atgcacaacc
     4081 gaccggctct gtgaagtgca gaatgtacgt gaatgggcta gttccatgac aatatgcact
     4141 gcatggctga cgtcgaccca cttgatgccc gtattctgct ggcgctcgac gacgagccgg
     4201 acgcttcggt gctgcaactc gcccgcaccc tggccatctc gcgcaacacc gtgcacgcaa
     4261 ggctgcagcg aatgctgcgc gagggggcgc tgctgggaca gagtcaacgc atcgcaccgg
     4321 ccagtctggg ctacccagtg gtcgggttcg tgtcgctgtc ggtgagccag gctcggggcg
     4381 agggcaccgc agcggccatg gcagaggtg
//
LOCUS       DAOSUG010000234         4397 bp    DNA     linear   ENV 10-JUN-2024
DEFINITION  MAG TPA_asm: Micropruina sp. isolate AMR_MDS_2195
            NODE_8523_length_4397_cov_4.518655, whole genome shotgun sequence.
ACCESSION   DAOSUG010000234 DAOSUG010000000
VERSION     DAOSUG010000234.1
DBLINK      BioProject: PRJNA925477
            BioSample: SAMN32864262
            Sequence Read Archive: SRR4236673
KEYWORDS    WGS; ENV; Metagenome Assembled Genome; MAG; Third Party Data; TPA;
            TPA:assembly.
SOURCE      Micropruina sp. (wastewater metagenome)
  ORGANISM  Micropruina sp.
            Bacteria; Bacillati; Actinomycetota; Actinomycetes;
            Propionibacteriales; Nocardioidaceae; Micropruina.
REFERENCE   1  (bases 1 to 4397)
  AUTHORS   Abdulkadir,N., Saraiva,J.P., Zhang,J., Stolte,S., Gillor,O.,
            Harms,H. and Rocha,U.
  TITLE     Genome-centric analyses of 165 metagenomes show that mobile genetic
            elements are crucial for the transmission of antimicrobial
            resistance genes to pathogens in activated sludge and wastewater
  JOURNAL   Microbiol Spectr 12 (3), e0291823 (2024)
   PUBMED   38289113
REFERENCE   2  (bases 1 to 4397)
  AUTHORS   Abdulkadir,N.
  TITLE     Direct Submission
  JOURNAL   Submitted (24-MAR-2023) Environmental Microbiology, Helmholtz
            Center for Environmental Research -UFZ, Permoserstr 15, Leipzig
            04318, Germany
COMMENT     The annotation was added by the NCBI Prokaryotic Genome Annotation
            Pipeline (PGAP). Information about PGAP can be found here:
            https://www.ncbi.nlm.nih.gov/genome/annotation_prok/
            
            ##Genome-Assembly-Data-START##
            Assembly Method        :: SPAdes v. 3.11.1
            Genome Representation  :: Full
            Expected Final Version :: Yes
            Genome Coverage        :: Not Applicable
            Sequencing Technology  :: Illumina MiSeq
            ##Genome-Assembly-Data-END##
            
            ##Genome-Annotation-Data-START##
            Annotation Provider               :: NCBI
            Annotation Date                   :: 05/04/2023 04:57:10
            Annotation Pipeline               :: NCBI Prokaryotic Genome
                                                 Annotation Pipeline (PGAP)
            Annotation Method                 :: Best-placed reference protein
                                                 set; GeneMarkS-2+
            Annotation Software revision      :: 6.5
            Features Annotated                :: Gene; CDS; rRNA; tRNA; ncRNA
            Genes (total)                     :: 2,776
            CDSs (total)                      :: 2,750
            Genes (coding)                    :: 2,714
            CDSs (with protein)               :: 2,714
            Genes (RNA)                       :: 26
            tRNAs                             :: 24
            ncRNAs                            :: 2
            Pseudo Genes (total)              :: 36
            CDSs (without protein)            :: 36
            Pseudo Genes (ambiguous residues) :: 0 of 36
            Pseudo Genes (frameshifted)       :: 2 of 36
            Pseudo Genes (incomplete)         :: 32 of 36
            Pseudo Genes (internal stop)      :: 2 of 36
            ##Genome-Annotation-Data-END##
FEATURES             Location/Qualifiers
     source          1..4397
                     /organism="Micropruina sp."
                     /mol_type="genomic DNA"
                     /submitter_seqid="NODE_8523_length_4397_cov_4.518655"
                     /isolate="AMR_MDS_2195"
                     /isolation_source="Activated sludge of municipal
                     wastewater treatment plants"
                     /db_xref="taxon:2737536"
                     /environmental_sample
                     /geo_loc_name="Canada"
                     /lat_lon="49.25 N 123.1 W"
                     /collection_date="2015-06-16"
                     /metagenome_source="wastewater metagenome"
                     /note="metagenomic"
     gene            complement(<1..328)
                     /locus_tag="PLF56_08435"
     CDS             complement(<1..328)
                     /locus_tag="PLF56_08435"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_008357359.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="lipoyl synthase"
                     /protein_id="HOQ53627.1"
                     /translation="MTLADDAVSPAPSSPPAGRKLLRLEVRNSQTPIERKPPWIRTTA
                     RMGPEYRGLMKLVQDEDLHTVCQEAGCPNIYECWEDREATFLIGGDSCTRRCDFCQIE
                     SRKPSGY"
     gene            complement(523..1245)
                     /gene="lipB"
                     /locus_tag="PLF56_08440"
     CDS             complement(523..1245)
                     /gene="lipB"
                     /locus_tag="PLF56_08440"
                     /EC_number="2.3.1.181"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_002527721.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_function: GO:0033819 - lipoyl(octanoyl) transferase
                     activity [Evidence IEA];
                     GO_process: GO:0009249 - protein lipoylation [Evidence
                     IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="lipoyl(octanoyl) transferase LipB"
                     /protein_id="HOQ53628.1"
                     /translation="MSAREVADDRPVQFQYLGLGEHRLSPYLPTWDYQRTIHARVASG
                     ELPPQVLFVEHEPVYTAGRRTEPHERPFDGTPVVDVDRGGKITWHGPGQLVGYPILFL
                     PRGIGVVDYVRRVEEALIRVLSDYGIHTGRVDGRIGVWLSAGATRPERKIAAIGIRVS
                     KQTTMHGFALNVTSQLAGFDNIVPCGIPDAGVTSMAAELDGPPPSLVEVAGALEPHLG
                     ELLALREYERAPEFASTSALTN"
     gene            complement(1791..2657)
                     /locus_tag="PLF56_08445"
     CDS             complement(1791..2657)
                     /locus_tag="PLF56_08445"
                     /inference="COORDINATES: ab initio
                     prediction:GeneMarkS-2+"
                     /note="Derived by automated computational analysis using
                     gene prediction method: GeneMarkS-2+."
                     /codon_start=1
                     /transl_table=11
                     /product="hypothetical protein"
                     /protein_id="HOQ53629.1"
                     /translation="MLPAELKELHVFSVQDARRVGMSDADLRAAVTRGVIVRLKRGWY
                     TAQPLPWPADRHRLLTGIEHQARPHTIPSHYSAAAMLGLPVFRPDWSTVHLMRTDDGA
                     PQNRDGLVIHKRVTPFDRSSVALAVAQTALLCPVSGLMAFDAALHDGLVTVGELQDVA
                     EAVTGWTGRANLAVVRRLGDGRRESPLESHTALVYDRWGLTVQPQFAVPGTTYTTDGR
                     IAGTNVLIECDGDAKYADQQVVIAEKTRENDMRALGWSMIRVTSEMLERPRLLYARTQ
                     TALKGAATASAA"
     gene            complement(3375..>4397)
                     /gene="sucB"
                     /locus_tag="PLF56_08450"
     CDS             complement(3375..>4397)
                     /gene="sucB"
                     /locus_tag="PLF56_08450"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_014847094.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_function: GO:0016746 - acyltransferase activity
                     [Evidence IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="2-oxoglutarate dehydrogenase, E2 component,
                     dihydrolipoamide succinyltransferase"
                     /protein_id="HOQ53630.1"
                     /translation="PAPATPAPAAPAASSDAGEVGYVTPLVRKLAAQHGVQLAGLSGT
                     GVGGRIRKQDVLAAAEAAKKAAEAPASAPSAEAPAAPAATAPAAKPSAPTSAPEGSKR
                     GTTEKISRMRSVIAKRMVESLQISAQLTGTVEVDLTNISKLRARAKDDFKKREGASLS
                     YLPFICKAVCEALKQFDKVNATMDVEAGTITYHNAEHLGIAVDTERGLLVPVIRDAGD
                     LSLAGLAKKIGEVAARSRGNKISPDELSGGTFTITNYGSAGTLFDTPIINQPQAAILG
                     TGALVKRPMVVSDPVLGDVIAVRDMMYLSLSYDHRLVDGADAARFLSVVKARLEAGDF
                     GGELGL"
BASE COUNT          623 a         1399 c         1614 g          761 t
ORIGIN      
        1 cgtagccgga cggcttgcgc gactcgatct ggcagaagtc gcagcgccgg gtgcacgagt
       61 cgccaccgat caggaaggtg gcctcgcggt cctcccagca ttcgtagatg ttggggcagc
      121 cggcttcctg gcacaccgtg tgcaggtctt cgtcctgcac cagcttcatc aggccgcggt
      181 attcagggcc catgcgggcg gtggtgcgga tccacggcgg cttgcgctcg atcggggtct
      241 ggctgttgcg cacctcgagt cgcaacagct tgcggccagc cggtgggctg ctgggcgcgg
      301 ggctgacggc gtcgtctgca agggtcaccg gctcatccta tccccgtcgg ccggtcgagc
      361 ctgcgggtga gcctgcgccg tgagcgaacc gggggcgggg ctgccggtgg cgaacgggag
      421 gcggggctac cgatgacgaa ccggggccgc catggggggt ttgccggcga acgatgtcgt
      481 ggccgcacgc gacggcggac ggcgtgtggg tgcgtccgat ggtcagttgg tgagggctga
      541 ggttgaggcg aactcgggcg cccgttcgta ctcacgcagc gcgagcagtt cacccaggtg
      601 cggttcgagc gcgccggcga cctcgaccag ggacggaggg ggaccgtcca gttcggctgc
      661 catcgaggtg acgccggcgt cggggattcc gcagggcacg atgttgtcga agccggccaa
      721 ctggctggtc acgttgagcg cgaacccgtg catcgtggtc tgcttgctga cccgaatgcc
      781 gatcgccgcg atcttgcgtt cgggccgagt ggcgccggcc gagagccaca cgccgatgcg
      841 tccgtccacc cgtccggtgt gaataccgta gtcggacagc actctgatca gggcctcctc
      901 gaccctgcgc acatagtcga ccacgccgat gccgcggggc agaaagagga tcgggtaacc
      961 gacgagttgg cccgggccgt gccaggtgat cttgccgccg cggtccacgt cgaccacggg
     1021 ggtgccgtcg aagggacgct cgtgcggctc ggtgcggcgt ccagcggtgt agacgggttc
     1081 gtgttcgacg aagagcacct gcggcggcag ttcgccggag gcgacccggg cgtggatggt
     1141 gcgctgatag tcccaggtgg gcaggtaggg gctgagccgg tgctcgccca ggcccaggta
     1201 ctgaaactgg acgggccggt cgtcggccac ctctcgcgcg gacacgtccg gcagtctatg
     1261 ccggacgagg cggacgggaa tgcctggtgc aggcctggcg cggtggcccg cccggcttcg
     1321 ggggcttacg cgatctcgag atcgctgcac actttcgggc aggccggacg atttcgtgtt
     1381 gcagcccgaa agtgtgtagc gcagccgaaa gcatgcaggg atctcgagat cgttgggacg
     1441 ggtcgtggcg ggtggtgggg gtggtggagg gctggacggt ccgcacgcgc gttgcgggtg
     1501 gtgggggctg actcgtcctg tcgcagggtg tgggtggagc cgaacgtcct gacgggaggg
     1561 cgcccgcgcg cggggtcaag cggccgatgc ggtggcggtt ggacggtcct gccgcgggtt
     1621 gcgggtggtg ggggctgact cgtcctgtcg cagggtgtgg gtggtgacga acgtcccgac
     1681 tggagggcgc ccgcgcacgg ggttaagcgg ccgatgcggt ggcggttgga cggtccgcag
     1741 gcaggtgcgg gtggtgagga acgtcctgac gggagggcgc ccgcgcgggg tcaagcggcc
     1801 gatgcggtgg cggcaccttt gagggcggtt tgggtgcgtg catagagcag ccgtggccgt
     1861 tccagcatct cgctggtgac tctgatcatc gaccagccca gtgctcgcat gtcgttctcg
     1921 cgggtcttct cggcgatgac cacctgctgg tcggcatact tcgcgtcgcc gtcgcattcg
     1981 atcaacacgt tcgtgccggc gatccgtccg tcggtggtgt aggtcgtgcc cgggacggcg
     2041 aactgtggct gcacggtcag tccccaccgg tcgtacacga gtgccgtgtg tgactccagg
     2101 ggtgactcgc gccgaccgtc accgagccgc cgtacgactg cgaggttggc gcggccggtc
     2161 catccggtga ccgcttcggc gacgtcctgt agttcgccga ccgtgacgag gccgtcgtgc
     2221 agcgcggcgt cgaaggccat caaacccgag accggacaca acagggcggt ctgcgccacg
     2281 gccagcgcga cgctcgacct gtcgaagggg gtcactcgct tgtggatgac gaggccatca
     2341 cggttctggg gtgcaccgtc gtcggtgcgc atcaggtgaa ccgtggacca gtcaggccgg
     2401 aagacgggca gaccaagcat tgccgcggcc gagtagtggc tcggaatggt gtgcgggcgg
     2461 gcctgatgct cgatgccggt gagtagccgg tgtcggtcgg ccggccaggg tagtggctgt
     2521 gcggtgtacc accccctctt gagtcggacg atcacccctc gcgtgacggc cgcacgcaga
     2581 tcggcgtcgg acatgccgac gcgtcgagcg tcctgcacgg agaagacgtg tagctctttc
     2641 agctctgccg gtagcacgcc agtatcatga cccctccagc cgggcgggag gcgtgccacc
     2701 gggcaatctg tggataactc gcggactgat gggcatcgac gatctcgaga tcgccacagg
     2761 gtttcggctt gccgcttcga tttcgtgttg tagggcggca tcgtcgggtt tgggcgaaat
     2821 cgtgcagcga tctcgagatc gtcggcgttc ccccggccgt ccaagagttc ccgtcacctc
     2881 caccgggcac ggcttaccgc tcgatggcct attgggggtg gcatcgtcgg tttgggtgca
     2941 atcgtccagc gatctcgaga tcgccacagg gtttcggctt gccgcttcga tttcgtgttg
     3001 tagggcgaca acgtcgggtt tgggcgaaac cgtccagcga tctcgagatc gtcggcgtcc
     3061 cctcgacctc ccgcgcgatc cgccaccggt cgtcggtgcc gtccgagccc tccgccggct
     3121 ccacctcctg ccgggcgaca gggcatcgca taatcctgac cgcgctgcgg cgcacgcagt
     3181 acgcccgctc gctggcgttg tcgtcctcgg cgccaggacc cgcgatcgac ttgtcttccg
     3241 ccctgcgaat cggacgcact gggcacgtcc gcgcggaacg cccagggtta tgcgacaccc
     3301 ccggtacaga ccagaaaccc cgccactctt cgccccattg ggtgaaaagt ggcggggtct
     3361 ggtccagccg aagctcagag gcccaactcg ccgccgaagt cgccggcctc caggcgtgcc
     3421 ttgaccaccg agaggaaccg ggccgcgtcg gcaccgtcca ccaggcggtg gtcgtagctg
     3481 agcgagagat acatcatgtc gcgcacggcg atcacgtcac ccagcaccgg gtcggacacc
     3541 accatcggac gcttcaccag cgccccggtg cccagaatgg ccgcctgcgg ctggttgatg
     3601 atcggggtgt cgaacagggt gccggccgag ccgtagttgg tgatcgtgaa ggtgccgcca
     3661 ctcagctcgt ccggcgagat cttgttgccg cgcgagcgtg ccgccacctc gccgatcttc
     3721 ttggccaggc cggccagact gaggtcgccc gcgtcacgga tcaccggcac cagcaggccg
     3781 cgctcggtgt cgaccgcgat gccgaggtgc tcggcgttgt ggtaggtgat ggtgccggcc
     3841 tcgacgtcca tcgtggcgtt caccttgtcg aactgcttca gcgcctcaca cacggccttg
     3901 cagatgaacg gcagatacga aagggatgca ccctcgcgct tcttgaagtc gtccttggcc
     3961 cgggcccgca gcttgctgat gttggtcagg tccacctcga ccgtgccggt caactgggcc
     4021 gagatctgca acgactcgac catgcgcttg gcgatcaccg agcgcatccg gctgatcttc
     4081 tcggtcgtgc cccgcttcga cccctctggt gcggacgtcg gagcggacgg cttcgccgcg
     4141 ggtgcggttg ccgccggtgc ggccggggcc tcggcggacg gagcggacgc gggtgcctcg
     4201 gcagccttct tggccgcctc ggccgcggcg agcacgtcct gcttgcggat gcgcccgccg
     4261 acaccggtgc cactcaggcc tgcgagttgc acgccgtgtt gcgcggcgag cttgcggacc
     4321 agcggcgtca cgtagcccac ctcacccgca tcggacgatg ccgcgggtgc ggccggagcc
     4381 ggagtcgccg gagccgg
//
LOCUS       DAOSUG010000235         4393 bp    DNA     linear   ENV 10-JUN-2024
DEFINITION  MAG TPA_asm: Micropruina sp. isolate AMR_MDS_2195
            NODE_8530_length_4393_cov_4.198018, whole genome shotgun sequence.
ACCESSION   DAOSUG010000235 DAOSUG010000000
VERSION     DAOSUG010000235.1
DBLINK      BioProject: PRJNA925477
            BioSample: SAMN32864262
            Sequence Read Archive: SRR4236673
KEYWORDS    WGS; ENV; Metagenome Assembled Genome; MAG; Third Party Data; TPA;
            TPA:assembly.
SOURCE      Micropruina sp. (wastewater metagenome)
  ORGANISM  Micropruina sp.
            Bacteria; Bacillati; Actinomycetota; Actinomycetes;
            Propionibacteriales; Nocardioidaceae; Micropruina.
REFERENCE   1  (bases 1 to 4393)
  AUTHORS   Abdulkadir,N., Saraiva,J.P., Zhang,J., Stolte,S., Gillor,O.,
            Harms,H. and Rocha,U.
  TITLE     Genome-centric analyses of 165 metagenomes show that mobile genetic
            elements are crucial for the transmission of antimicrobial
            resistance genes to pathogens in activated sludge and wastewater
  JOURNAL   Microbiol Spectr 12 (3), e0291823 (2024)
   PUBMED   38289113
REFERENCE   2  (bases 1 to 4393)
  AUTHORS   Abdulkadir,N.
  TITLE     Direct Submission
  JOURNAL   Submitted (24-MAR-2023) Environmental Microbiology, Helmholtz
            Center for Environmental Research -UFZ, Permoserstr 15, Leipzig
            04318, Germany
COMMENT     The annotation was added by the NCBI Prokaryotic Genome Annotation
            Pipeline (PGAP). Information about PGAP can be found here:
            https://www.ncbi.nlm.nih.gov/genome/annotation_prok/
            
            ##Genome-Assembly-Data-START##
            Assembly Method        :: SPAdes v. 3.11.1
            Genome Representation  :: Full
            Expected Final Version :: Yes
            Genome Coverage        :: Not Applicable
            Sequencing Technology  :: Illumina MiSeq
            ##Genome-Assembly-Data-END##
            
            ##Genome-Annotation-Data-START##
            Annotation Provider               :: NCBI
            Annotation Date                   :: 05/04/2023 04:57:10
            Annotation Pipeline               :: NCBI Prokaryotic Genome
                                                 Annotation Pipeline (PGAP)
            Annotation Method                 :: Best-placed reference protein
                                                 set; GeneMarkS-2+
            Annotation Software revision      :: 6.5
            Features Annotated                :: Gene; CDS; rRNA; tRNA; ncRNA
            Genes (total)                     :: 2,776
            CDSs (total)                      :: 2,750
            Genes (coding)                    :: 2,714
            CDSs (with protein)               :: 2,714
            Genes (RNA)                       :: 26
            tRNAs                             :: 24
            ncRNAs                            :: 2
            Pseudo Genes (total)              :: 36
            CDSs (without protein)            :: 36
            Pseudo Genes (ambiguous residues) :: 0 of 36
            Pseudo Genes (frameshifted)       :: 2 of 36
            Pseudo Genes (incomplete)         :: 32 of 36
            Pseudo Genes (internal stop)      :: 2 of 36
            ##Genome-Annotation-Data-END##
FEATURES             Location/Qualifiers
     source          1..4393
                     /organism="Micropruina sp."
                     /mol_type="genomic DNA"
                     /submitter_seqid="NODE_8530_length_4393_cov_4.198018"
                     /isolate="AMR_MDS_2195"
                     /isolation_source="Activated sludge of municipal
                     wastewater treatment plants"
                     /db_xref="taxon:2737536"
                     /environmental_sample
                     /geo_loc_name="Canada"
                     /lat_lon="49.25 N 123.1 W"
                     /collection_date="2015-06-16"
                     /metagenome_source="wastewater metagenome"
                     /note="metagenomic"
     gene            complement(108..614)
                     /locus_tag="PLF56_08455"
     CDS             complement(108..614)
                     /locus_tag="PLF56_08455"
                     /EC_number="2.3.1.-"
                     /inference="COORDINATES: protein motif:HMM:NF025054.2"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_function: GO:0008080 - N-acetyltransferase activity
                     [Evidence IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="GNAT family N-acetyltransferase"
                     /protein_id="HOQ53631.1"
                     /translation="MLMQRALPLPEPVGPADVTVRPQSIDDVERLGALYLVSYPQGVA
                     AVDLEDAVEEMEGVFEGEYGTPVESASLVVETSDGALVGCVQTVTSPPWEGIPAVPFV
                     IELFVHPDHRRRGLGSLLLLSAARACHEQGWSSLALNVQEETAGEATHLYRGLGFSEI
                     TPVAVGEG"
     gene            902..1654
                     /locus_tag="PLF56_08460"
     CDS             902..1654
                     /locus_tag="PLF56_08460"
                     /inference="COORDINATES: protein motif:HMM:NF012730.2"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="ion channel"
                     /protein_id="HOQ53632.1"
                     /translation="MERLNRWENVMEWPLMATAVVFLLAYALPIAAPGAPTWFAATCE
                     VIVWIAWAVFAVDYLCRFAIAPAKWPFVRANLLDLAVVALPLLRPLRLIRLLALLSIL
                     NRAGTRNLRGRVVAYTAGATVLALVVGALAITDAERGEPGANIATLSDGFWWAVTTMT
                     TVGYGDRYPVTATGRFVAVALMVSGIAVLGIVTATLASWLVQRVAEVTETEEAATRAQ
                     VQQLSEEVTTLRIALERMERTLRGFLVEPPAT"
     gene            complement(2023..2107)
                     /locus_tag="PLF56_08465"
     tRNA            complement(2023..2107)
                     /locus_tag="PLF56_08465"
                     /product="tRNA-Tyr"
                     /inference="COORDINATES: profile:tRNAscan-SE:2.0.12"
                     /note="Derived by automated computational analysis using
                     gene prediction method: tRNAscan-SE."
                     /anticodon=(pos:complement(2071..2073),aa:Tyr,seq:gta)
     gene            2189..2680
                     /locus_tag="PLF56_08470"
     CDS             2189..2680
                     /locus_tag="PLF56_08470"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_012923767.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="YajQ family cyclic di-GMP-binding protein"
                     /protein_id="HOQ53633.1"
                     /translation="MAGDSSFDVVSKFDQQELENAVNMTAKEVRNRYDFKGTGAAIKL
                     AGESIELEAEGEERVKAVLDVLQTMLVRRKIDLKSLKPGEPRLSGKIWKLTATMQQGI
                     SGDDARKIAKLIRDEGPKGVRTQVQGDELRVFSKKRDDLQAVQQLIKGADYDFAVQFA
                     NYR"
     gene            2913..3596
                     /locus_tag="PLF56_08475"
     CDS             2913..3596
                     /locus_tag="PLF56_08475"
                     /inference="COORDINATES: protein motif:HMM:NF021425.2"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="DUF2127 domain-containing protein"
                     /protein_id="HOQ53634.1"
                     /translation="MRRADPAVARVIGEPSARVELNELPQAVRGAHGRKFALLRLLAV
                     ERLLKGLSLITAAIVAYHVASRRASLLSWLEELLISAAPLGQQLGLHLTTAAPVRLIE
                     STLGGDGEPLRLAGLLLLAYGALQLVEGIGLWGGWRWAEYLAAIATALFVPLEIYEIA
                     EHPTVLKVAALVVNLLAVGYLVYKGRLFGTRGGYRAYLAEVRDHTEMADVLRALGRPT
                     TELSSRTIV"
     gene            3645..>4393
                     /locus_tag="PLF56_08480"
     CDS             3645..>4393
                     /locus_tag="PLF56_08480"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_013861020.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="exodeoxyribonuclease III"
                     /protein_id="HOQ53635.1"
                     /translation="MTTLRIATMNVNGIRAAERRGFGSWLQRREPHVVALQELRCPSG
                     EVPLQAWPDHRLTYHPGLLAGRNGVGVLTRWAPSAVRLGFGNRVFDSEGRYLELDLEP
                     PDAPPLTIGSLYLPKGGNPADGPAFAAKQQRKLRFMASFGQYLTGARRAARRLGREFL
                     VMGDFNIAHTPDDLANPSANRRYPGFMPDEREWFGRLLGPRTLVDVVRALHPEVKGPY
                     SWWRWGPQAWQRGTGWRIDYQLATPTLAATAR"
BASE COUNT          703 a         1461 c         1509 g          720 t
ORIGIN      
        1 accgcagacc ttctccttac catggagacg ctctgccgac tgagctatag gggcctggac
       61 cgcgtgaaac actacaggag ggatcgggtc ggcctcaaat cggctggtca gccctcgcca
      121 acggcgaccg gtgtgatctc actgaaaccc agaccacggt acaggtgcgt ggcttcgccc
      181 gcggtctcct cctgcacgtt gagcgccagg ctcgaccagc cctgctcgtg acacgcacgg
      241 gcggcgctca acagcagcag cgagcccagg ccgcggcgcc gatgatcggg atgcacgaac
      301 aactcgatca cgaacggcac cgccggaatg ccctcccagg gcgggctcgt gaccgtttgg
      361 acgcagccga ccagtgcccc gtccgacgtc tcgacgacga gcgaggcgga ttcgaccggc
      421 gtgccgtact cgccctcgaa cacgccttcc atctcttcga ccgcgtcttc gagatcgacc
      481 gcggcgaccc cctgggggta tgagaccagg tacagggcac ccagccgctc gacgtcgtcg
      541 atcgactgcg gacggacggt gacgtcggct ggccctaccg gctctggcaa gggaagcgct
      601 cgctgcatga gcatgccgcc agtctaggtg aaacgccccc ggagagccgt gagggaatcg
      661 ctgaaggcgg ctgtgtctac gtgtcgattc cgtgcgtgga cggcgggcgt caccccccga
      721 ccggccaaag ctgcggcact ggccggacga ccagccgagg cgctgcggtc ggcccgcaac
      781 ttcggcgcca cgggccctcg gacgcacggc tctgcggttc acttcttgcg gcactccgcg
      841 ccacttcggg accttggacg atcctcgcgc ggtccagttg gcgcaacgct agcgtgcagc
      901 catggagcgc ctgaatcgct gggagaacgt catggagtgg cccctgatgg ccacggccgt
      961 cgtgtttctg ctcgcatacg ccctgcccat cgccgcacca ggcgcaccaa cctggtttgc
     1021 ggcgacgtgt gaggtcatcg tgtggatcgc ctgggcagta ttcgcagtgg actacctctg
     1081 tcggtttgct atcgcgcctg cgaagtggcc cttcgtcagg gcgaatctgc tcgacctcgc
     1141 cgtggtcgcg ctaccccttc tgcgaccctt gcgcctcatc cgcctcctgg cactgctgtc
     1201 aatcctcaac cgtgccggga cgcgcaacct gcggggccgg gtggtcgcgt acacggcggg
     1261 cgccacggtg ttggcccttg tcgtcggtgc gctggcgatc accgatgctg agcgaggtga
     1321 accgggtgcg aacatcgcga cgctgagcga cggattctgg tgggcggtta ccacgatgac
     1381 gacagtgggg tacggagacc gctacccggt gaccgcgacc ggccgattcg tggccgtcgc
     1441 cctaatggtc tccggtatcg ccgtactcgg aatcgtcacc gccacgctcg catcgtggct
     1501 ggtgcaacgg gtcgcggagg tcaccgagac ggaagaagcg gccacgcgag cacaggtcca
     1561 gcaactctcg gaggaggtca ctacgctccg gatcgcgttg gaacgcatgg agcggactct
     1621 gcgcggattt ctggttgagc cacctgccac gtgatcgccg ccgaacatgc atgaaggacg
     1681 gtggtttccg gcgccggcct cgactgaagc tcgatgcgcg acgccatgga acgagcgggg
     1741 cgaacagttt gtcccagacc ccttcggcca cctggggggc ggtgaggcca ggcctcgata
     1801 ccgtggagtc gacggccagg tgcagtgcgc ccgatatggc ccgggctgtc tcagccctca
     1861 gcaaatcgca gacgtcgaga gtctcccccc gtggcggctg actagcccgt tcacagccgc
     1921 gacggtgcgg ttcgcagcac ttgggcgaat cacggccatg tgcagccgtc gttcccgaat
     1981 gctaaaaccc ccagtgaaca tcgagttcac tgggggtttt actggtggca ggtgtaggat
     2041 tcgaacctac gtaggcgatg ccgacggttt tacagaccgc tccctttggc cgctcgggca
     2101 acctgccgtg gttgggccga ggaccaagat agcaacattg ggccggggcg gaccaatccg
     2161 cgacacatga aggtgggtca cacacacgat ggctggggat agctcgttcg acgtggtcag
     2221 caagttcgac cagcaagaac tcgagaacgc ggtcaacatg accgcgaaag aggtgcgcaa
     2281 ccgctacgac ttcaagggca ccggggccgc gatcaagctg gccggcgagt cgatcgaact
     2341 cgaggccgag ggcgaagagc gggtgaaggc ggtgctggac gtgctgcaga ccatgctggt
     2401 gcgccgcaag atcgacctca agtcgctcaa acccggtgag ccacggttgt cgggcaagat
     2461 ctggaagctg accgccacca tgcagcaggg catctcgggc gacgacgccc gcaagatcgc
     2521 caagctgatc cgcgacgagg ggcccaaagg cgtccgtacc caggtgcagg gcgacgagct
     2581 gcgggtgttc agcaagaagc gtgacgacct gcaggccgtc cagcagctga tcaagggcgc
     2641 cgactacgac tttgcggttc agttcgccaa ctaccggtag cggaacgcac gcaatgcagc
     2701 ctggtgcgga tggcgcccag gcgtccgtcg gacagctgag tcgtcgcgat tcgctctggg
     2761 tgctccggcg ctgcggccgg cagggtcacg tgctcggttg ggtggccgac ccggccgtcg
     2821 catagcatct ggagcggccc gccgtcgatc tggacgggga cggcgccgac gacacttccg
     2881 ccctggtccg ctgcctgcgc tgtggcacgt gggtgcgccg cgccgatccc gcggtcgccc
     2941 gggtgatcgg cgagccgtcg gcgcgggttg agctgaacga gctgccgcag gccgttcggg
     3001 gtgcgcatgg acgcaagttc gccctgctgc ggctgttggc cgtggaacga ctgctcaagg
     3061 gcctgtcgct gatcaccgcc gcgatcgtgg cctaccatgt cgcgagccgg cgggcgtccc
     3121 tgctttcgtg gttggaggag ctgctgatct cggccgcgcc actgggccag cagttgggac
     3181 tgcatctgac cacggccgca cccgtccgct tgatcgagag cactctcggc ggggatggcg
     3241 aaccgctgag gttggctggt ctgctgctgc tcgcctacgg tgcgctgcaa ctcgtcgagg
     3301 gcatcggcct gtggggtggt tggcgctggg ccgagtacct ggccgcgatc gcgaccgcac
     3361 tcttcgttcc gctggagatc tacgagatcg ccgagcaccc cacggtgctc aaggtcgccg
     3421 cactggtggt gaatctgctg gcggtgggct acctggtcta caagggccgg ctgttcggca
     3481 cccgaggcgg ctaccgggcc tatctggccg aggtgcgcga ccacaccgag atggcggacg
     3541 tgctgcgtgc actcgggcgt ccgaccacgg aactgagcag ccgcaccatc gtgtgaaccc
     3601 accacccggg cgagcggcgc gacccagtcg gctagcttgg cgccgtgacc accctgcgga
     3661 tcgcgaccat gaacgtgaac ggcatccgcg ccgccgagcg ccggggcttc ggttcgtggt
     3721 tgcagcgacg cgagccgcac gtcgtcgccc tgcaggaact gcggtgtccg tccggcgagg
     3781 tgccgctgca ggcctggccc gatcaccggt tgacctacca ccccgggctg ctggccggac
     3841 gcaacggggt cggagtgctg acccgttggg cgccgtccgc ggtgcggttg ggtttcggca
     3901 accgtgtgtt cgactccgag ggtcgctatc tggagctcga cctcgaaccc ccggacgccc
     3961 cgccgctcac catcggctcg ctctacctgc ccaagggcgg caatcccgcc gacggcccgg
     4021 ccttcgcggc caagcagcag cgcaagctgc ggttcatggc gtcgttcggc cagtacctga
     4081 ccggtgcccg gcgcgccgcc cgccggctcg ggcgcgagtt tctggtgatg ggcgacttca
     4141 acatcgcgca caccccggac gacctggcga acccgagcgc caatcgtcgc taccccgggt
     4201 tcatgcccga cgagcgagag tggttcgggc ggctgctcgg cccgcgcacc ctggtggacg
     4261 tggtccgcgc cctgcacccg gaagtcaagg gtccgtactc gtggtggcgg tgggggccac
     4321 aggcgtggca gcgcggtacc ggttggcgga tcgactacca gttggcgaca cccacgctcg
     4381 cggcgacggc ccg
//
LOCUS       DAOSUG010000236         4357 bp    DNA     linear   ENV 10-JUN-2024
DEFINITION  MAG TPA_asm: Micropruina sp. isolate AMR_MDS_2195
            NODE_8602_length_4357_cov_3.892143, whole genome shotgun sequence.
ACCESSION   DAOSUG010000236 DAOSUG010000000
VERSION     DAOSUG010000236.1
DBLINK      BioProject: PRJNA925477
            BioSample: SAMN32864262
            Sequence Read Archive: SRR4236673
KEYWORDS    WGS; ENV; Metagenome Assembled Genome; MAG; Third Party Data; TPA;
            TPA:assembly.
SOURCE      Micropruina sp. (wastewater metagenome)
  ORGANISM  Micropruina sp.
            Bacteria; Bacillati; Actinomycetota; Actinomycetes;
            Propionibacteriales; Nocardioidaceae; Micropruina.
REFERENCE   1  (bases 1 to 4357)
  AUTHORS   Abdulkadir,N., Saraiva,J.P., Zhang,J., Stolte,S., Gillor,O.,
            Harms,H. and Rocha,U.
  TITLE     Genome-centric analyses of 165 metagenomes show that mobile genetic
            elements are crucial for the transmission of antimicrobial
            resistance genes to pathogens in activated sludge and wastewater
  JOURNAL   Microbiol Spectr 12 (3), e0291823 (2024)
   PUBMED   38289113
REFERENCE   2  (bases 1 to 4357)
  AUTHORS   Abdulkadir,N.
  TITLE     Direct Submission
  JOURNAL   Submitted (24-MAR-2023) Environmental Microbiology, Helmholtz
            Center for Environmental Research -UFZ, Permoserstr 15, Leipzig
            04318, Germany
COMMENT     The annotation was added by the NCBI Prokaryotic Genome Annotation
            Pipeline (PGAP). Information about PGAP can be found here:
            https://www.ncbi.nlm.nih.gov/genome/annotation_prok/
            
            ##Genome-Assembly-Data-START##
            Assembly Method        :: SPAdes v. 3.11.1
            Genome Representation  :: Full
            Expected Final Version :: Yes
            Genome Coverage        :: Not Applicable
            Sequencing Technology  :: Illumina MiSeq
            ##Genome-Assembly-Data-END##
            
            ##Genome-Annotation-Data-START##
            Annotation Provider               :: NCBI
            Annotation Date                   :: 05/04/2023 04:57:10
            Annotation Pipeline               :: NCBI Prokaryotic Genome
                                                 Annotation Pipeline (PGAP)
            Annotation Method                 :: Best-placed reference protein
                                                 set; GeneMarkS-2+
            Annotation Software revision      :: 6.5
            Features Annotated                :: Gene; CDS; rRNA; tRNA; ncRNA
            Genes (total)                     :: 2,776
            CDSs (total)                      :: 2,750
            Genes (coding)                    :: 2,714
            CDSs (with protein)               :: 2,714
            Genes (RNA)                       :: 26
            tRNAs                             :: 24
            ncRNAs                            :: 2
            Pseudo Genes (total)              :: 36
            CDSs (without protein)            :: 36
            Pseudo Genes (ambiguous residues) :: 0 of 36
            Pseudo Genes (frameshifted)       :: 2 of 36
            Pseudo Genes (incomplete)         :: 32 of 36
            Pseudo Genes (internal stop)      :: 2 of 36
            ##Genome-Annotation-Data-END##
FEATURES             Location/Qualifiers
     source          1..4357
                     /organism="Micropruina sp."
                     /mol_type="genomic DNA"
                     /submitter_seqid="NODE_8602_length_4357_cov_3.892143"
                     /isolate="AMR_MDS_2195"
                     /isolation_source="Activated sludge of municipal
                     wastewater treatment plants"
                     /db_xref="taxon:2737536"
                     /environmental_sample
                     /geo_loc_name="Canada"
                     /lat_lon="49.25 N 123.1 W"
                     /collection_date="2015-06-16"
                     /metagenome_source="wastewater metagenome"
                     /note="metagenomic"
     gene            complement(154..579)
                     /locus_tag="PLF56_08485"
     CDS             complement(154..579)
                     /locus_tag="PLF56_08485"
                     /inference="COORDINATES: protein motif:HMM:NF019428.2"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="TadE/TadG family type IV pilus assembly protein"
                     /protein_id="HOQ53636.1"
                     /translation="MRRDGERGMMVAVETAIILPILLGMVGLLVVLATGALAQMTVNS
                     AASQAARAASLERDTGTATASARQVAASTLTESGTTCRTSNVAVNAAGLRAPLGTPAV
                     VSVTVTCTVDLQVGLPGFPSTRTLTATKSSPVDTYRSRR"
     gene            complement(585..1409)
                     /locus_tag="PLF56_08490"
     CDS             complement(585..1409)
                     /locus_tag="PLF56_08490"
                     /inference="COORDINATES: ab initio
                     prediction:GeneMarkS-2+"
                     /note="Derived by automated computational analysis using
                     gene prediction method: GeneMarkS-2+."
                     /codon_start=1
                     /transl_table=11
                     /product="hypothetical protein"
                     /protein_id="HOQ53637.1"
                     /translation="MNEDTIVVSVNVPGSSGGGGRGQGSAGGGGSAPSGCFTGGGAEL
                     PCTQWGLAWRPDLQCYARVKPYQPPLTDPVWEGNTTGTIIECRTGTEGGWSNTWDLWA
                     AAAQLAAPPDPAELARRAVERMQLEAIRMGTFPEQAERAPRDLGYVGWNAWMWVSNPT
                     PSTWGPITKSVSEAGYTVTATGQVDKVVWDMGDGTTITCGQGKPWRSIWVHNEPSPDC
                     GHMYERDGEYTITATSHWVVNWSGIGQSGTITTQLSDTATLRIAEVQVVNVVTPES"
     gene            complement(1502..2092)
                     /locus_tag="PLF56_08495"
     CDS             complement(1502..2092)
                     /locus_tag="PLF56_08495"
                     /inference="COORDINATES: ab initio
                     prediction:GeneMarkS-2+"
                     /note="Derived by automated computational analysis using
                     gene prediction method: GeneMarkS-2+."
                     /codon_start=1
                     /transl_table=11
                     /product="hypothetical protein"
                     /protein_id="HOQ53638.1"
                     /translation="MMITRLALAALAALFLLGACTPEPAETQSPTASTPAATPSITPS
                     PSPSPTASPSPTSTLSPEQEAAQAAAVEYFRALNAVRSDLEADFQQVADITTGTHTSA
                     ESQVIGEYRSKGIVQVGERTYTYKGVGPVVEAGGVKSVEVRVCSDSTSSDMVDAEGNS
                     VLDPARLRFVDFRLDVVQVGNSWRINGGQSEAVESC"
     gene            complement(2106..2495)
                     /locus_tag="PLF56_08500"
     CDS             complement(2106..2495)
                     /locus_tag="PLF56_08500"
                     /inference="COORDINATES: protein motif:HMM:NF019428.2"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="TadE/TadG family type IV pilus assembly protein"
                     /protein_id="HOQ53639.1"
                     /translation="MCRPTSPPDQDQRGLSSSVLATILFPLVISALWLTMQWAMLTWA
                     QATAHAAAQDGARAAAALNATGTHGQATATRAADNGSIDNLHVTAQRAARLTTVTITG
                     TALQVIPGFPVTIQVTATSPTQRLTPS"
     gene            complement(2470..2646)
                     /locus_tag="PLF56_08505"
     CDS             complement(2470..2646)
                     /locus_tag="PLF56_08505"
                     /inference="COORDINATES: ab initio
                     prediction:GeneMarkS-2+"
                     /note="Derived by automated computational analysis using
                     gene prediction method: GeneMarkS-2+."
                     /codon_start=1
                     /transl_table=11
                     /product="hypothetical protein"
                     /protein_id="HOQ53640.1"
                     /translation="MLALYSYLTATLHTRVRVRDERGLSQSTENAILLAGAVGIAVLI
                     ISAITAYVQANLPS"
     gene            complement(2666..3535)
                     /locus_tag="PLF56_08510"
     CDS             complement(2666..3535)
                     /locus_tag="PLF56_08510"
                     /inference="COORDINATES: protein motif:HMM:NF012694.2"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="type II secretion system F family protein"
                     /protein_id="HOQ53641.1"
                     /translation="MLSGATITAGLVMLVAGLVPAPTHLKDAIGRLQPRITLDTLSMG
                     EKADSEVRLGRWAERHLPAIIWGTAPEKDLALLGRTKATFYGSKLISGILGLLVVPVI
                     SVATMLLGLPIPIAVPVLGSLGFAAIMWFLPNNELRDQAAKAREEFSYALVAFVEMVA
                     LERLSGATVPEALLRAADTGDSWVFQRLATVLRRTQYTGQNPWDALADMGHHYDLPDL
                     VDLADIMRLAGADGTRIYSSLRARGAAMRNATLNQQISRANADGERIAIPVTLLVLVL
                     AITLVVPAVLRMI"
     gene            complement(3553..>4357)
                     /locus_tag="PLF56_08515"
     CDS             complement(3553..>4357)
                     /locus_tag="PLF56_08515"
                     /inference="COORDINATES: protein motif:HMM:NF012694.2"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=2
                     /transl_table=11
                     /product="type II secretion system F family protein"
                     /protein_id="HOQ53642.1"
                     /translation="PVTPPKAPRIRRRRRLSKVRGVALAGLAAGALIAVVTGWLVALV
                     IAPAVSVMVYRMVTGRATNRIERLNALADFARALGGVLTVGRGLEQAILHAARSAPAA
                     LAPEIGRLAARVRANVATATALRLFADELDDATGDLFAATLILAAERRGPGVASAMQG
                     LAESIDEDVKARRQVETEQQKARTAARMITIISALLLGGLFLAGSYVEPYRTPLGQVL
                     LTVFMSMYLAALVWMGRISAPKPLPRFIGVTGQHATANTAVAGKQGGVG"
BASE COUNT          644 a         1332 c         1660 g          721 t
ORIGIN      
        1 cggcgtggcc ggcgaaatcg atcccgatgc cgatggtcac gatgaacgcc accatcgcga
       61 ccgacaccca caccgacacc attccccgct ccccacgctt cttcccatgc ccggaagaac
      121 cccgaccggg gcgggtcagc gcgtgacggg ggttcatcgt cggctccggt aggtgtcgac
      181 cggtgacgat ttcgtggcgg tgagggtgcg ggtggagggg aagccgggca ggccgacctg
      241 gaggtcgacg gtgcaggtca cggtcaccga caccacagcc ggcgtcccga ggggggcgcg
      301 gaggccggcg gcgttcacgg cgacgttgga ggtgcggcag gtggtgccgg actcggtcaa
      361 ggtggaggcg gccacctggc gtgcggaggc ggtggcggtg ccggtgtcgc gttcgaggga
      421 ggcggcgcgg gcggcctggg acgcagcgga gttcaccgtc atctgcgcca acgctccggt
      481 ggccagcacg accagcagac cgaccatgcc gagcaggatg ggcaggatga tggcggtttc
      541 gacggccacc atcatccccc gctcaccatc acgcctcatc gtcgtcagct ttcgggggtg
      601 acgacgttga cgacctggac ttcggcgatc cgcagggtag ccgtgtcgga cagctgcgtg
      661 gtgatggtgc cgctctgtcc gatgccggac cagttgacca cccagtggga ggtggcggtg
      721 atcgtgtact cgccatccct ctcgtacatg tggccgcagt ccggggatgg ttcgttgtgt
      781 acccagatcg agcgccaggg cttgccttgg ccgcaggtga tggtggtgcc gtcgcccatg
      841 tcccacacga ccttgtcgac ctgtccggtg gcggtgacgg tgtagccggc ctcagacacg
      901 gacttggtga tcggccccca cgtcgacggg gtcgggttgg agacccacat ccacgcattc
      961 cagccgacgt agccgaggtc tctgggtgcg cgttcggctt gttcggggaa ggtgcccatc
     1021 cggatcgctt cgagttgcat ccgctccacc gcccgcctgg cgagctccgc gggatccggc
     1081 ggagccgcca actgcgccgc agccgcccac aagtcccagg tgttgctcca cccaccctca
     1141 gtgccggtcc ggcactcgat gatcgtcccg gtcgtgttgc cctcccagac tgggtcggtc
     1201 agtggcggct ggtagggctt gacgcgggcg tagcactgca ggtcgggccg ccaggccagg
     1261 ccccactggg tgcagggcaa ctccgcaccg ccaccggtga agcagcccga cggagccgaa
     1321 ccaccaccgc cagcactccc ctgcccgcgg ccaccacccc ccgacgagcc gggcacattc
     1381 accgacacca cgatcgtgtc ttcgttcaag aaggcgccgc cggagccttc tgcagaggct
     1441 ggccgaactg atgccccgac gaggagggtt ccgaggacga ccaataccgg taggactcgt
     1501 ctcagcatga ttcgaccgcc tcactctgtc ctccgttgat ccgccagctg ttgccgacct
     1561 ggactacgtc gagcctgaag tcgacgaagc gcaggcgggc agggtcgagg accgagttgc
     1621 cctcagcgtc gaccatgtcg ctgctggtgc tgtcgctaca cacgcggact tccacggact
     1681 tgacgcctcc ggcctccacc accggaccca cccccttgta ggtgtacgtg cgctcgccga
     1741 cttggacgat ccccttactg cggtactcac cgatgacctg cgactctgcc gaagtgtgcg
     1801 tccctgttgt gatgtcggcc acctgctgaa agtcagcctc caggtcgctc cggaccgcgt
     1861 tgagtgcgcg gaagtactcc accgctgccg cttgggcggc ttcttgttcg ggtgacaggg
     1921 tgctcgtggg cgacggtgag gcggtggggg acgggctcgg tgacggcgtg atcgagggtg
     1981 tcgcggccgg tgtgctggcc gtcggtgact gcgtctccgc cggctctggg gtgcaggcac
     2041 cgagcaggaa cagtgcggcc agggccgcga gtgcgagtcg tgtgatcatc atctcttcgt
     2101 ctccgtcagg atggggtgag tcgttgggtg ggtgaggtgg ccgtgacctg gatggtgacg
     2161 gggaagccgg ggatgacttg tagggcggtg ccggtgatgg tgactgtggt gagtcgggcg
     2221 gctcgttggg cggtgacgtg gaggttgtcg atggatccgt tgtcggcggc tcgggtggct
     2281 gtggcctgac cgtgtgttcc ggtggcgttg agggctgctg ctgctctggc tccgtcttgt
     2341 gctgctgcat gggcggttgc ttgtgcccag gtgagcatgg cccactgcat cgtcagccag
     2401 agggcgctga tgacgagcgg gaagaggatg gtggccagga cggaggagct gaggccccgt
     2461 tggtcctggt caggagggga ggttggcctg cacatatgcg gtgatcgcgg agatgatcag
     2521 gacggcgatc ccgacggcgc cggccaggag gatcgcgttc tcggttgact gggacagacc
     2581 ccgctcatcc cgcacccgga cacgcgtgtg gagggtggcg gtgaggtagc tgtagagggc
     2641 aagcatcgaa gtggttcctt tcgggttaga tcatgcggag gactgcgggg acgacgaggg
     2701 tgatcgccag gacgaggacg agcagggtga cggggatggc gatgcgttcc ccgtcggcgt
     2761 tggcgcggct gatttgttgg ttgagggtgg cgttgcgcat ggcggcgccg cgggcgcgta
     2821 gtgaggagta gatgcgggtg ccgtcggcgc cggccaggcg catgatgtcg gccaggtcga
     2881 ccagatcggg caggtcgtag tggtggccca tgtccgccag cgcgtcccac gggttctggc
     2941 cggtgtattg ggtgcggcgc agcacggtgg ccaggcgttg gaacacccag gagtcgccgg
     3001 tgtcggcggc gcggagcagg gcttcgggga cggtggcgcc ggagagccgc tcgagggcca
     3061 ccatttccac gaacgcgacc agggcgtagg agaattcttc gcgggctttg gcggcttggt
     3121 cgcggagttc gttgttggga aggaaccaca tgatggccgc gaacccgagc gagccgagga
     3181 cgggcaccgc gatcgggatg gggagaccga gcaacatggt cgccacgctg atcacgggca
     3241 cgacgaggag gccgaggatg ccggagatca gtttggagcc gtagaacgtg gccttggtgc
     3301 gtcccaggag ggcgaggtcc ttctccggtg cggtgcccca gatgatcgcg gggaggtggc
     3361 gctcggccca gcggccgagg cgaacctccg aatctgcctt ctcccccatg ctcagggtgt
     3421 cgagggtgat gcggggttgg aggcggccga tggcgtcctt cagatgggtc ggggccggca
     3481 ccaggccggc gacgagcatg accaggccgg cggtgatggt ggcgcccgag agcatggcca
     3541 gctgcagcgc ggtcatccca cacccccctg cttcccggcg acagcggtgt tggctgtggc
     3601 gtgctgtccg gtgacgccga tgaaccgcgg caggggcttg ggggcggaga tgcggcccat
     3661 ccacaccaag gcggccaggt acatggacat gaacacggtc agcaggacct ggcccagcgg
     3721 ggtgcggtag ggctcgacgt agctgccggc caggaacagg cccccgagga ggagcgcgga
     3781 gatgatggtg atcattctgg cggcggtgcg ggccttctgc tgctcggttt cgacctggcg
     3841 gcgggccttg acgtcctcgt cgatggattc ggccaggccc tgcatggcgg aggccacgcc
     3901 ggggccgcgg cgttcggcgg cgaggatcag ggtggcggcg aacaggtccc ctgtcgcatc
     3961 atccaactcg tcggcaaaca acctgagcgc ggtggcggtg gcgacgttgg cgcggacccg
     4021 cgcagccaat ctgccgattt cgggggccag cgcggcgggg gcggagcggg cggcgtgcag
     4081 gatcgcctgc tccaggccgc ggccgacggt caggacgccg ccgagggcgc gggcgaagtc
     4141 cgccagcgcg ttgagccgct cgatgcggtt cgtcgccctg cccgtgacca tcctgtagac
     4201 catcaccgac acggccggtg cgatgaccag ggcgacgagc cacccggtca ccacggcgat
     4261 cagggcgccg gcggccagcc cggcgagggc aacgccacgg accttcgaca gccggcggcg
     4321 ccggcggatg cggggcgcct tcgggggggt gacgggg
//
LOCUS       DAOSUG010000237         4354 bp    DNA     linear   ENV 10-JUN-2024
DEFINITION  MAG TPA_asm: Micropruina sp. isolate AMR_MDS_2195
            NODE_8605_length_4354_cov_5.340079, whole genome shotgun sequence.
ACCESSION   DAOSUG010000237 DAOSUG010000000
VERSION     DAOSUG010000237.1
DBLINK      BioProject: PRJNA925477
            BioSample: SAMN32864262
            Sequence Read Archive: SRR4236673
KEYWORDS    WGS; ENV; Metagenome Assembled Genome; MAG; Third Party Data; TPA;
            TPA:assembly.
SOURCE      Micropruina sp. (wastewater metagenome)
  ORGANISM  Micropruina sp.
            Bacteria; Bacillati; Actinomycetota; Actinomycetes;
            Propionibacteriales; Nocardioidaceae; Micropruina.
REFERENCE   1  (bases 1 to 4354)
  AUTHORS   Abdulkadir,N., Saraiva,J.P., Zhang,J., Stolte,S., Gillor,O.,
            Harms,H. and Rocha,U.
  TITLE     Genome-centric analyses of 165 metagenomes show that mobile genetic
            elements are crucial for the transmission of antimicrobial
            resistance genes to pathogens in activated sludge and wastewater
  JOURNAL   Microbiol Spectr 12 (3), e0291823 (2024)
   PUBMED   38289113
REFERENCE   2  (bases 1 to 4354)
  AUTHORS   Abdulkadir,N.
  TITLE     Direct Submission
  JOURNAL   Submitted (24-MAR-2023) Environmental Microbiology, Helmholtz
            Center for Environmental Research -UFZ, Permoserstr 15, Leipzig
            04318, Germany
COMMENT     The annotation was added by the NCBI Prokaryotic Genome Annotation
            Pipeline (PGAP). Information about PGAP can be found here:
            https://www.ncbi.nlm.nih.gov/genome/annotation_prok/
            
            ##Genome-Assembly-Data-START##
            Assembly Method        :: SPAdes v. 3.11.1
            Genome Representation  :: Full
            Expected Final Version :: Yes
            Genome Coverage        :: Not Applicable
            Sequencing Technology  :: Illumina MiSeq
            ##Genome-Assembly-Data-END##
            
            ##Genome-Annotation-Data-START##
            Annotation Provider               :: NCBI
            Annotation Date                   :: 05/04/2023 04:57:10
            Annotation Pipeline               :: NCBI Prokaryotic Genome
                                                 Annotation Pipeline (PGAP)
            Annotation Method                 :: Best-placed reference protein
                                                 set; GeneMarkS-2+
            Annotation Software revision      :: 6.5
            Features Annotated                :: Gene; CDS; rRNA; tRNA; ncRNA
            Genes (total)                     :: 2,776
            CDSs (total)                      :: 2,750
            Genes (coding)                    :: 2,714
            CDSs (with protein)               :: 2,714
            Genes (RNA)                       :: 26
            tRNAs                             :: 24
            ncRNAs                            :: 2
            Pseudo Genes (total)              :: 36
            CDSs (without protein)            :: 36
            Pseudo Genes (ambiguous residues) :: 0 of 36
            Pseudo Genes (frameshifted)       :: 2 of 36
            Pseudo Genes (incomplete)         :: 32 of 36
            Pseudo Genes (internal stop)      :: 2 of 36
            ##Genome-Annotation-Data-END##
FEATURES             Location/Qualifiers
     source          1..4354
                     /organism="Micropruina sp."
                     /mol_type="genomic DNA"
                     /submitter_seqid="NODE_8605_length_4354_cov_5.340079"
                     /isolate="AMR_MDS_2195"
                     /isolation_source="Activated sludge of municipal
                     wastewater treatment plants"
                     /db_xref="taxon:2737536"
                     /environmental_sample
                     /geo_loc_name="Canada"
                     /lat_lon="49.25 N 123.1 W"
                     /collection_date="2015-06-16"
                     /metagenome_source="wastewater metagenome"
                     /note="metagenomic"
     gene            complement(<1..756)
                     /gene="cydB"
                     /locus_tag="PLF56_08520"
     CDS             complement(<1..756)
                     /gene="cydB"
                     /locus_tag="PLF56_08520"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_011754415.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_function: GO:0016682 - oxidoreductase activity, acting
                     on diphenols and related substances as donors, oxygen as
                     acceptor [Evidence IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="cytochrome d ubiquinol oxidase subunit II"
                     /protein_id="HOQ53643.1"
                     /translation="MNVEPTTLQLLWFCLIAVLWIGFLVLEGFDYGVAMLIPVLGRTD
                     KERRVLVNTIGPVWDGNEVWLLTAGGATFAAFPGWYATLFSGLYIPLFLILIALIVRG
                     LAFEYRAKRPEQSWKNRWDWGAAIGSFVVSLVLGVGFSNFVKGMPVQVAEGRQAVHVF
                     TGGFWSLFSPFALLGGVVLVVLFLFHGAIFLALKTRGDIHERAKAFALKVGLVAIGGG
                     GVWLLWSNLAYGTGLPGWILMLVAAAGLVFAWFM"
     gene            complement(773..2347)
                     /locus_tag="PLF56_08525"
     CDS             complement(773..2347)
                     /locus_tag="PLF56_08525"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_013863351.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_component: GO:0016020 - membrane [Evidence IEA];
                     GO_component: GO:0070069 - cytochrome complex [Evidence
                     IEA];
                     GO_function: GO:0009055 - electron transfer activity
                     [Evidence IEA];
                     GO_function: GO:0046872 - metal ion binding [Evidence
                     IEA];
                     GO_process: GO:0019646 - aerobic electron transport chain
                     [Evidence IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="cytochrome ubiquinol oxidase subunit I"
                     /protein_id="HOQ53644.1"
                     /translation="MDQVTLARWQFGITTVYHWFFVPVTLALSFIVAIFQTLWYRTRD
                     DKYLRLTKFYGKLFLINFAMGVVTGIVQEFQFGMNWSEYSRFVGDIFGAPLAFEALLA
                     FFLESTFLGLWIFGWDRLPKRLHLMCIWLAAIGTNLSAIFILAANSFMQNPQGATYNP
                     LTDRAEMTDFGAVLTNPVLLWSLPHTLMASWLTAAALVAAVSGWHLSKLNRAGVSDDA
                     AGAPSKDADDVSGDTDYADASGVTTTDNSVAAHRFAATFAAIAMIVAGVGLLITGDEA
                     GKIMTQVQPMKMAAAEALWESAGGDGSGAPFSVFTIGTLDGSRPIVQVEIPNLLSFLA
                     TGTWDGRVQGINELKAAYAEGQLVDPSNKLQQAYADHMRAWGVETHTPIIPVTYWSFR
                     LMIGLGMAAIAVALLMLWYLRKGGLPPRAPWWTLLMVLMPLGPLFANSFGWIFTEMGR
                     QPWIVSGVLPTEAGISPGVTVLELWISLIGYTLVYGALAVVEVGLLLKYIRLGLPQVG
                     PVEQIQDEHDVLSFAY"
     gene            2403..2717
                     /locus_tag="PLF56_08530"
     CDS             2403..2717
                     /locus_tag="PLF56_08530"
                     /inference="COORDINATES: protein motif:HMM:NF015898.2"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_function: GO:0003677 - DNA binding [Evidence IEA];
                     GO_process: GO:0045892 - negative regulation of
                     DNA-templated transcription [Evidence IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="BlaI/MecI/CopY family transcriptional regulator"
                     /protein_id="HOQ53645.1"
                     /translation="MPVFGDLEQAVMEVLWASPAAMSVRDVHAALSRDRELAYTTVMT
                     VMDRLAKKSQLKRELDGRAWQYRPTYSWAQLASNEIIALLETGPVEQRLGVLDDVRSR
                     VD"
     gene            3059..4186
                     /gene="uxuA"
                     /locus_tag="PLF56_08535"
     CDS             3059..4186
                     /gene="uxuA"
                     /locus_tag="PLF56_08535"
                     /EC_number="4.2.1.8"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_017936445.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_function: GO:0008927 - mannonate dehydratase activity
                     [Evidence IEA];
                     GO_process: GO:0006064 - glucuronate catabolic process
                     [Evidence IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="mannonate dehydratase"
                     /protein_id="HOQ53646.1"
                     /translation="MKMGFRWYGEGNDTVSLDDIRQIPGVETVVWSLHHKQAGDVWHE
                     AEIAAELAKIAAISDAAAARGVTKRLDGDVVESVNVHESIKLGRTVFGLSRDEAIANY
                     ITTIERLGRAGVKVICYNFMPVFDWLRTDLWHPLPDGSTALYFEKAVVDQLTPEKLIA
                     DMEANSGGLTLPGWEPERLAGFSELNAAYEGVTHEDMYRTYQYFLDAVIPTCEQYDVK
                     LGVHPDDPPFDLFGWPRVVSTMEDLGRVLSLNESPYNGLTLCLGSFSANATQAPRAVE
                     AVQTYLPRIHFSHVRNVKHYPNGDFSEVAHRACEGDVDTVGVMRAYAEADFTGYIRPD
                     HGRHLWTENTTNKPRPGYGLYDRALGIQYLFGVWDAVRSGR"
BASE COUNT          801 a         1396 c         1484 g          673 t
ORIGIN      
        1 catgaaccag gcgaacacca gcccggcggc cgccaccagc atcaggatcc agcccggcag
       61 cccggtgccg taggccaggt tcgaccacag cagccacacg ccgccaccac cgatggcgac
      121 caggccgacc ttgagggcga aggccttggc ccgctcgtga atgtcgccgc gggtcttcag
      181 cgccagaaag atcgcgccgt gaaacaggaa cagcaccacg agcaccacgc cgccgagcag
      241 ggcgaacggg ctgaacagcg accagaagcc gccggtgaac acgtggacgg cctggcgtcc
      301 ttcggcgacc tgcaccggca tgcccttgac gaagttcgag aagccgacgc ccagcaccag
      361 cgacaccacg aacgagccga tcgcggcgcc ccagtcccag cggttcttcc acgactgttc
      421 gggccgcttg gcccggtact cgaaggccag gccacgcacg atcagcgcga tcagaatcag
      481 gaacagcggt atgtacagcc ccgagaacaa cgtggcgtac cagcccggaa aggcggcgaa
      541 cgtggcaccg cccgcggtca gcagccagac ctcgttgccg tcccagaccg ggccgatggt
      601 gttgaccagg acgcgccgtt ccttgtccgt cctgcccagt acgggaatca acatggccac
      661 gccgtagtcg aaaccctcga gcaccagaaa gccgatccac aacacggcga tcaggcagaa
      721 ccacagcagc tgaagggtgg tgggttcaac gttcataccg atcatcaccc tctcagtagg
      781 cgaaggacag cacgtcgtgc tcgtcctgga tctgttcgac ggggccgacc tgcggcaacc
      841 cgagccggat gtatttgagc agcagcccga cctcgaccac ggccagggca ccgtagacca
      901 gggtgtagcc gatcaggctg atccacagtt cgagaacggt cacaccgggc gagattccgg
      961 cttcggtggg cagcacgccc gacacgatcc acggttggcg gcccatctcg gtgaagatcc
     1021 agccgaacga gttggcgaac agcgggccga gcggcatcag caccatcagc aacgtccacc
     1081 acggggcgcg gggcggtaac ccgcccttgc gcaggtacca cagcatcagc agggcgaccg
     1141 cgatggcggc catgccgagg ccgatcatca gccggaagct ccagtaggtg accggaatga
     1201 tcggcgtgtg ggtttcgacc ccccaggcgc gcatgtggtc ggcgtaggcc tgctgcagct
     1261 tgttggacgg gtccaccaac tgaccctcgg cataggccgc cttcagttcg ttgatgccct
     1321 gcacgcgtcc gtcccaagtg ccggtggcca ggaacgacag caggttcggt atctcgacct
     1381 gcacaatcgg gcgcgaaccg tccagggtgc cgatggtgaa gaccgagaac ggggcgccgc
     1441 tgccgtcgcc gccggcgctc tcccacagcg cctccgcggc ggccatcttc atcggctgca
     1501 cctgcgtcat gatcttgccg gcctcatcgc cggtgatcag caacccgacg ccggcgacga
     1561 tcatggcgat cgcggcgaag gtggcggcga agcggtgtgc ggccacgctg ttgtcggtcg
     1621 tggtgacacc ggacgcatcc gcgtagtcgg tgtcgccgga cacatcgtcg gcgtccttgg
     1681 acggtgcccc ggccgcgtcg tccgaaacgc cggcgcggtt cagcttcgac aggtgccagc
     1741 cggagacggc agccaccagg gcggcggcgg tcagccagga cgccatcagc gtgtgcggca
     1801 gcgaccacag cagcaccggg ttggtcagca cggcgccgaa gtcggtcatc tcggcgcgat
     1861 cggtcagggg gttgtaggtg gcaccctgcg ggttctgcat gaacgagttg gccgcaagga
     1921 tgaagatggc gctcaggttg gtgccgatcg ctgcgagcca gatgcacatc aggtgcagac
     1981 gcttgggcag ccgatcccag ccgaagatcc acaggccgag aaacgtcgac tccagaaaga
     2041 acgcgagcag ggcctcgaag gccaggggag cgccgaagat gtcgccgacg aatcgtgagt
     2101 attcgctcca gttcatgccg aactggaact cctgcacgat gccggtgacg acacccatgg
     2161 cgaagttgat gagaaagagc ttgccgtaga acttcgtcaa ccgaaggtac ttgtcatcgc
     2221 gcgtgcggta ccagagcgtc tggaagatcg cgacgatgaa cgagagggcg agggtgaccg
     2281 gaacgaagaa ccagtgataa acggtggtga tgccgaactg ccaccgtgcg agtgtgacct
     2341 gatccatggc ggcaatgtac taccggctgt cgtagatggg caatgaggct taggctggcc
     2401 ccatgcccgt gttcggcgac ttggaacaag cagtcatgga ggtgttgtgg gcgagtcccg
     2461 cggcgatgtc cgtgcgcgac gttcacgctg ccctcagccg cgatcgcgag ctcgcctaca
     2521 ccacggtgat gaccgtgatg gaccggctgg ccaagaagag ccagctgaaa cgtgagttgg
     2581 acggccgggc gtggcagtac cgtccgacct attcgtgggc gcagctggcg agcaacgaga
     2641 tcatcgcgct gctcgagacc ggtcctgtcg agcagcggct aggagtgctg gacgacgtcc
     2701 gttcccgggt cgactgagga accaccgatc aatcgccgct cgaccctgtc gagaccctcg
     2761 tgagcgggcg ttgagacagc gcaacaacgg atccctgcgt tgatcagcgc ctccccaacg
     2821 gactgtccgg tgggttctcc aggtgcgtgc cgatcaatcg ggatcgtcgc atgaccgtca
     2881 agcctcggct cgcgtggtct gcgcacgtgc gaccagcggt ggctcagagt ctcgcctggt
     2941 ccggcctctt ccgcgaccat gggcggtctg gcgctccgca cccaataggc tgcttgcgca
     3001 ccacgtgcga cggcgcgcga tcggggcggt ttcgtccgag cagtcaggag gttcgaccat
     3061 gaagatgggc tttcgttggt acggcgaggg caacgacacc gtgtcgttgg acgacatccg
     3121 gcagattccc ggcgtcgaga ccgtggtgtg gagcctgcac cacaagcagg ccggcgacgt
     3181 gtggcacgag gccgagatcg ccgccgagct ggcgaagatc gccgcgatct cggacgcggc
     3241 cgccgcccgc ggggtgacca agcggctgga cggcgacgtg gtcgagtcgg tcaacgtgca
     3301 cgagtcgatc aagctcggac gcaccgtgtt cgggctgagt cgcgacgagg cgatcgccaa
     3361 ctacatcacc acgatcgaac gcctcggccg ggccggggtg aaggtgatct gctacaactt
     3421 catgccggtg ttcgactggc tgcgcaccga tctgtggcat ccgctgccgg acggttcgac
     3481 agcgctgtac ttcgagaagg ccgtggtcga ccagttgacg cccgagaagc tgatcgccga
     3541 catggaagcc aactccggcg ggctcaccct gcccggctgg gagccggagc ggctggccgg
     3601 tttcagcgaa ctcaacgccg cctacgaggg cgtcacccac gaagacatgt accgcaccta
     3661 ccagtacttc ctggacgcgg tgatccccac ctgcgagcag tacgacgtga agttgggggt
     3721 gcacccggac gatccgccgt tcgacctgtt cggttggccg cgggtggtgt cgacgatgga
     3781 ggatctgggt cgggtgttgt cgctgaacga gagcccgtac aacgggttga cgctgtgcct
     3841 ggggtcgttc tcggccaacg ccacgcaggc gccccgggcg gtcgaggcgg tgcagacgta
     3901 cctgccgcgc atccacttct cgcacgtccg caacgtcaag cactacccga acggtgactt
     3961 cagcgaggtg gcgcaccggg cctgcgaggg cgacgtcgac accgtcgggg tgatgcgggc
     4021 gtacgccgag gcggacttca ccggctacat acgtcccgat cacggccgcc acctgtggac
     4081 ggagaacacc accaacaaac cccgtccggg ctacgggttg tacgaccgcg cgctgggcat
     4141 tcagtacctg ttcggggtct gggacgcggt gcgctccggc cgctgaccca gcccgattct
     4201 gctgtgggtg gagcccgctc gtcgtcccgg gggatgtggg tggagcccgc tggtcgtccc
     4261 gggggatgtg ggtggagccc gctggtcgtc ccggcggatg gtgggtgggg cccgctggtc
     4321 gtcccggcgg atggtgggtg gggcccgctg gtcg
//
LOCUS       DAOSUG010000238         4340 bp    DNA     linear   ENV 10-JUN-2024
DEFINITION  MAG TPA_asm: Micropruina sp. isolate AMR_MDS_2195
            NODE_8634_length_4340_cov_4.318086, whole genome shotgun sequence.
ACCESSION   DAOSUG010000238 DAOSUG010000000
VERSION     DAOSUG010000238.1
DBLINK      BioProject: PRJNA925477
            BioSample: SAMN32864262
            Sequence Read Archive: SRR4236673
KEYWORDS    WGS; ENV; Metagenome Assembled Genome; MAG; Third Party Data; TPA;
            TPA:assembly.
SOURCE      Micropruina sp. (wastewater metagenome)
  ORGANISM  Micropruina sp.
            Bacteria; Bacillati; Actinomycetota; Actinomycetes;
            Propionibacteriales; Nocardioidaceae; Micropruina.
REFERENCE   1  (bases 1 to 4340)
  AUTHORS   Abdulkadir,N., Saraiva,J.P., Zhang,J., Stolte,S., Gillor,O.,
            Harms,H. and Rocha,U.
  TITLE     Genome-centric analyses of 165 metagenomes show that mobile genetic
            elements are crucial for the transmission of antimicrobial
            resistance genes to pathogens in activated sludge and wastewater
  JOURNAL   Microbiol Spectr 12 (3), e0291823 (2024)
   PUBMED   38289113
REFERENCE   2  (bases 1 to 4340)
  AUTHORS   Abdulkadir,N.
  TITLE     Direct Submission
  JOURNAL   Submitted (24-MAR-2023) Environmental Microbiology, Helmholtz
            Center for Environmental Research -UFZ, Permoserstr 15, Leipzig
            04318, Germany
COMMENT     The annotation was added by the NCBI Prokaryotic Genome Annotation
            Pipeline (PGAP). Information about PGAP can be found here:
            https://www.ncbi.nlm.nih.gov/genome/annotation_prok/
            
            ##Genome-Assembly-Data-START##
            Assembly Method        :: SPAdes v. 3.11.1
            Genome Representation  :: Full
            Expected Final Version :: Yes
            Genome Coverage        :: Not Applicable
            Sequencing Technology  :: Illumina MiSeq
            ##Genome-Assembly-Data-END##
            
            ##Genome-Annotation-Data-START##
            Annotation Provider               :: NCBI
            Annotation Date                   :: 05/04/2023 04:57:10
            Annotation Pipeline               :: NCBI Prokaryotic Genome
                                                 Annotation Pipeline (PGAP)
            Annotation Method                 :: Best-placed reference protein
                                                 set; GeneMarkS-2+
            Annotation Software revision      :: 6.5
            Features Annotated                :: Gene; CDS; rRNA; tRNA; ncRNA
            Genes (total)                     :: 2,776
            CDSs (total)                      :: 2,750
            Genes (coding)                    :: 2,714
            CDSs (with protein)               :: 2,714
            Genes (RNA)                       :: 26
            tRNAs                             :: 24
            ncRNAs                            :: 2
            Pseudo Genes (total)              :: 36
            CDSs (without protein)            :: 36
            Pseudo Genes (ambiguous residues) :: 0 of 36
            Pseudo Genes (frameshifted)       :: 2 of 36
            Pseudo Genes (incomplete)         :: 32 of 36
            Pseudo Genes (internal stop)      :: 2 of 36
            ##Genome-Annotation-Data-END##
FEATURES             Location/Qualifiers
     source          1..4340
                     /organism="Micropruina sp."
                     /mol_type="genomic DNA"
                     /submitter_seqid="NODE_8634_length_4340_cov_4.318086"
                     /isolate="AMR_MDS_2195"
                     /isolation_source="Activated sludge of municipal
                     wastewater treatment plants"
                     /db_xref="taxon:2737536"
                     /environmental_sample
                     /geo_loc_name="Canada"
                     /lat_lon="49.25 N 123.1 W"
                     /collection_date="2015-06-16"
                     /metagenome_source="wastewater metagenome"
                     /note="metagenomic"
     gene            complement(2..87)
                     /locus_tag="PLF56_08540"
     tRNA            complement(2..87)
                     /locus_tag="PLF56_08540"
                     /product="tRNA-Leu"
                     /inference="COORDINATES: profile:tRNAscan-SE:2.0.12"
                     /note="Derived by automated computational analysis using
                     gene prediction method: tRNAscan-SE."
                     /anticodon=(pos:complement(51..53),aa:Leu,seq:gag)
     gene            492..1823
                     /locus_tag="PLF56_08545"
     CDS             492..1823
                     /locus_tag="PLF56_08545"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_012921427.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="M20/M25/M40 family metallo-hydrolase"
                     /protein_id="HOQ53647.1"
                     /translation="MSAQSNPQASQPDTEVVQLCSELIRIDTSNYGPEGSAGERLAAE
                     YVAEKLAEVGIESEMFEPAPTRTNLVAHWQPEGCDPSLPPLLIHGHTDVVPAQASDWQ
                     VDPFAGEIKDGCVWGRGAVDMKDFDAMVLSVVRDRQRTGRAPRRPIRLVFTADEEAGS
                     GVGAHWLVENRPDLIADCTEAIGEVGGFSLTVRDDLRLYLVQVAEKGLAWLNLIADGT
                     AGHGSMRNHDNAVTALAGAVARIGAYEWPHRIHPAQQAFLDAVEDALGVQINADSVEE
                     SLARLGTVARMVGATMSNTANPTMLQAGYKVNVIPGRATAGLDGRFIPGYEQEFFDTI
                     TELLGDKVRYEEVSRQDAVETEFSGALVEAMQTCLSAEDPQAKAVPFMMSGGTDGKAW
                     QKLGVRCFGFAPLQLPTDLDFVAMFHGVDERVPTDSLQFGCRVLDRFLDQA"
     gene            2019..2204
                     /locus_tag="PLF56_08550"
     CDS             2019..2204
                     /locus_tag="PLF56_08550"
                     /inference="COORDINATES: ab initio
                     prediction:GeneMarkS-2+"
                     /note="Derived by automated computational analysis using
                     gene prediction method: GeneMarkS-2+."
                     /codon_start=1
                     /transl_table=11
                     /product="hypothetical protein"
                     /protein_id="HOQ53648.1"
                     /translation="MTVGGSFPGTGAFMSYTTDQLRKDNALGEYELSPEEEQITIIQA
                     PSAPASAHDESVDEPKQ"
     gene            2275..3234
                     /locus_tag="PLF56_08555"
     CDS             2275..3234
                     /locus_tag="PLF56_08555"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_015070791.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="aldo/keto reductase"
                     /protein_id="HOQ53649.1"
                     /translation="MKRRAVGTSGLQVSRLGLGTLTWGRDVQARDARALLLRFVEAGG
                     TLVDTAAAYGNGDAERIIGRLIHTDLTRDDLVIATKGGFGVREGKRVVDTSRTALLAD
                     LAGSLRRLHTDHVDLWQLHAWGPAPLDESLAAVDHAVTAGMVRYAGVCNFVGWQTAQA
                     ATWQAAFPGRAPIVSTQVEYSLLARRAEVEVIPAVRAFGMGLFPWSPLGRGVLAGRYR
                     SGIPRDSRGGQAHFSWFVEPYLEARSRAVVEAVATAADGLDLTCAQVALLWARDAPGV
                     TAPLIGPRTSEQLGQLLQTEELALPQEIVSALDDVSGGLNAGR"
     gene            complement(3543..>4340)
                     /locus_tag="PLF56_08560"
     CDS             complement(3543..>4340)
                     /locus_tag="PLF56_08560"
                     /EC_number="3.6.1.27"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_017935359.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_component: GO:0016020 - membrane [Evidence IEA];
                     GO_function: GO:0050380 - undecaprenyl-diphosphatase
                     activity [Evidence IEA];
                     GO_process: GO:0016311 - dephosphorylation [Evidence IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="undecaprenyl-diphosphate phosphatase"
                     /protein_id="HOQ53650.1"
                     /translation="EGITEFLPISSTGHLTVAEKLMGVQIDDPSVTAYTAIIQVGAMV
                     ASIIYFWADIVRFATGWFKGLRDSSARGADYRMGWAIIAGFIPTAVIALLAESLITGV
                     LRSLWAVVIGLIGWSVVMFIGDRVGKQNRGEETITVRDGIILGTLQSLALVPGVSRSG
                     ATITGGLLLGLDRVAATRMSFFLGIPTLVASGLYQAVKEAKHIADGVGWASTAIGTVV
                     SGVVAYASIAWLLRYVSKNNFTVFIIYRVALALVIIALLVTQVISAT"
BASE COUNT          725 a         1454 c         1471 g          690 t
ORIGIN      
        1 gtgtccgaga gaggacttga acctccacgc ccgataaagg gcactagcac ctcaagctag
       61 cgcgtctacc tattccgcca cccggacaag ggtgcacccg gaccgaccgg gcaaccacgt
      121 cagcatatcg gccctcccca tgccgctcaa ctcgacctcc gagcccacgc ctgacagccc
      181 aacttcgcga tcaagacacg tgaggcacgc agtcagattt cgcgatcacc ttgacatgag
      241 gcaaggcgct cgcgcgctcg tgcacgtccg ccgaccagag tgccgaaccc ggccaggcac
      301 ggtccaacct tgtacaaacc ctcgacatcg gttggtgtga ccatctattc tgggtgaagc
      361 ggatcacccc ctgacgttct gatccgagcc tgcgccgtcc ggacatcccc ccgtccggac
      421 ggcgcttcgc tgcccgggtg gtgcttggac tcgccggcct gcggcccgct caggtgcggc
      481 aggatggggg catgagcgcg cagagcaatc cacaggccag ccaacccgac accgaggtcg
      541 tgcagctgtg tagtgagctg atccgcatcg acacctccaa ctacggcccc gagggttccg
      601 cgggggagcg cctcgccgcc gagtacgtgg ctgagaagct ggctgaggtg ggtatcgagt
      661 ctgaaatgtt cgagccggcg cccacgcgaa ccaacctggt cgcgcactgg cagcccgagg
      721 gctgcgaccc ctcgctgccg ccgttgttga tccacggtca caccgacgtg gtgccggcgc
      781 aggcgtccga ctggcaggtg gatcccttcg ccggtgagat caaggacggc tgcgtctggg
      841 gtcgcggggc ggtggacatg aaggatttcg acgccatggt gttgtccgtg gtccgcgacc
      901 gccaacgcac cggacgggcg ccgcgacgtc cgattcgatt ggtcttcacc gcggacgaag
      961 aggccggcag cggcgtgggc gcgcactggc tggtcgagaa ccgccccgac ctcatcgcgg
     1021 actgcaccga ggccatcgga gaggtcggcg gattctcgct cacggtgcgt gatgacctgc
     1081 ggctgtacct ggtgcaggtc gccgaaaagg ggctcgcctg gctcaacctg atcgcggatg
     1141 gcaccgccgg gcacgggtcg atgcgcaacc atgacaacgc ggtgaccgcc ctggcgggcg
     1201 ctgtcgcacg gatcggcgcc tacgagtggc cgcaccggat ccatcccgcc cagcaggcat
     1261 ttctcgacgc cgtcgaggac gccctggggg tgcagatcaa cgccgattcg gtcgaggagt
     1321 cgctcgcccg gctcggcacg gtggcgcgca tggtgggcgc caccatgtcg aacacggcga
     1381 accccaccat gctccaggcg ggttacaagg tgaacgtgat tccaggccgg gccacggccg
     1441 ggctggacgg ccgattcatt ccgggctacg agcaggagtt cttcgacacc atcactgaac
     1501 tgctcggtga caaggtgcgt tatgaagagg tcagccgtca ggacgcggtc gagaccgagt
     1561 tctctggcgc cctggtcgag gcgatgcaga cctgcctgag cgccgaggat ccgcaggcca
     1621 aagcggtccc gttcatgatg agcggcggca ccgacggtaa ggcctggcag aagctgggcg
     1681 tgcgttgctt cggcttcgca ccgctgcagt tgccgaccga cctcgatttc gtggccatgt
     1741 tccatggcgt ggacgagcgg gtgcccacgg actccttgca gttcggttgc cgggtgctcg
     1801 accgttttct cgaccaggcc tgacccgagg gacggactct ggtctggacg gcgactgccg
     1861 tccagagtcg gcagcacccc gcacggctgg atgcggcgcc gaaacacccc tccggctacc
     1921 gagggtccgt gcgctccgaa acacccctcc ggctaccgag ggtccgtgcg ccccgcagcg
     1981 ctacgctgat ccgaccacct ccatcagaag ggcgtccgat gaccgtcggc ggcagctttc
     2041 ccggtaccgg ggccttcatg tcgtacacga ccgatcagtt gcgcaaagac aatgcgctcg
     2101 gcgaatacga actgtccccc gaagaggaac agatcacgat catccaggct ccttccgctc
     2161 cggcgtccgc ccacgacgag tcggtcgacg aaccgaagca gtaggcgccg attccggcgg
     2221 ctccggcggc tgggcgccgt ccggtgttgt cggtggtccg tttagggtgt cgttgtgaaa
     2281 cgacgcgccg tgggcaccag tggtttgcag gtgtcgcggc tcggtctggg cacgctcacc
     2341 tggggtcggg acgtgcaggc tcgcgacgcg cgcgccctgc tgctccgctt cgtcgaggcc
     2401 ggcggcacgc tggtcgacac cgccgccgcc tacggcaacg gcgacgccga gcgcatcatc
     2461 ggccggctga tccacaccga cctgacccgc gacgacctgg tgatcgccac caagggtggt
     2521 ttcggggtgc gcgagggcaa gcgggtggtc gacacgtcga gaaccgcgtt gctcgccgac
     2581 ctggccgggt cgcttcgccg gctgcacacc gatcacgtcg acctgtggca gttgcacgct
     2641 tggggccccg cgccccttga cgaatcgctg gccgcggtcg atcacgccgt caccgccggc
     2701 atggtgcgtt acgcgggggt gtgcaacttc gtcggctggc agaccgcgca ggccgccacc
     2761 tggcaggctg cctttcccgg acgcgcgccc atcgtcagca cgcaggtgga gtactcgttg
     2821 ctggccaggc gagcggaggt ggaggtgatc cccgccgtcc gggcgttcgg aatgggtctg
     2881 tttccctggt cgccgctggg ccggggtgtg ctcgccggac ggtaccgctc aggtattcca
     2941 cgcgactcgc gcggtggcca ggcgcatttc tcgtggttcg tcgaaccgta cctggaggct
     3001 cgctcgcgcg ccgtggtcga agcggttgcc acggcggcgg acggtctcga cctgacgtgc
     3061 gcacaggttg cactgctctg ggcccgggac gctccgggtg tgacggcccc gctgatcggg
     3121 ccgcggacgt ccgaacagct ggggcagttg ttgcagaccg aggaactcgc cctgccacag
     3181 gagatcgtca gtgccctgga cgacgtcagc ggcgggttga acgcagggcg ctgaaccgtt
     3241 ggccgacggt gtcgaggcgg gtcggccggc cgcgggtctg gatttcgacg gcctccatcg
     3301 gcggcgacct gggattcggc ctccagcgcc gttcagtcct tcagccggcg tccagtaacg
     3361 gcactccggg cggatggccg gagtgggaca accctcacaa catcgtgccc agcccaatca
     3421 gctcctgatc gcccgacgag gaggaccagc agatcaggcg gatggccgga gtgggacaac
     3481 cctcgcaaca tcgtgccgag ccccaatcag ttcctgatcg cccgaccatg aggaccccca
     3541 gatcaggtgg cgctgatcac ctgggtgacc agcagcgcga tgatcaccag tgcgagggcg
     3601 acgcggtaga tgatgaaaac ggtgaagttg ttcttggaca cgtagcgcag cagccacgcg
     3661 atggacgcgt aggcgaccac acccgagacc accgtgccga tggcggtgga ggcccagccc
     3721 accccgtccg caatgtgttt ggcctctttg accgcctggt agaggcccga ggcgaccagc
     3781 gtcggaatgc cgaggaagaa gctcatccgg gtggcggcga cccggtcgag gccgagcagc
     3841 aggccgccgg tgatggtcgc gcccgagcgg gacacgccgg gcaccagcgc cagtgattgc
     3901 agcgtgccga gaatgatgcc gtcgcgcacg gtgatggttt cttcgccgcg gttctgcttg
     3961 ccgacccggt caccgatgaa catcaccacc gaccaaccga tcaggccgat cactacggcc
     4021 cacaggctgc gcagcacgcc cgtgatcagc gactccgcca acagtgcgat caccgcggtg
     4081 ggaatgaagc cggcgatgat cgcccagccc atgcggtagt cggccccgcg ggcggacgaa
     4141 tcgcggagcc ccttgaacca gccggtcgcg aaccggacga tgtcggccca gaagtagatg
     4201 atggacgcca ccatggcacc gacctgaatg atggccgtgt acgcggtgac gctggggtcg
     4261 tcgatctgca cccccatcag cttctcggcg acggtcaggt gacctgtgga cgagatgggc
     4321 agaaactccg tgatgccctc
//
LOCUS       DAOSUG010000239         4336 bp    DNA     linear   ENV 10-JUN-2024
DEFINITION  MAG TPA_asm: Micropruina sp. isolate AMR_MDS_2195
            NODE_8641_length_4336_cov_3.604765, whole genome shotgun sequence.
ACCESSION   DAOSUG010000239 DAOSUG010000000
VERSION     DAOSUG010000239.1
DBLINK      BioProject: PRJNA925477
            BioSample: SAMN32864262
            Sequence Read Archive: SRR4236673
KEYWORDS    WGS; ENV; Metagenome Assembled Genome; MAG; Third Party Data; TPA;
            TPA:assembly.
SOURCE      Micropruina sp. (wastewater metagenome)
  ORGANISM  Micropruina sp.
            Bacteria; Bacillati; Actinomycetota; Actinomycetes;
            Propionibacteriales; Nocardioidaceae; Micropruina.
REFERENCE   1  (bases 1 to 4336)
  AUTHORS   Abdulkadir,N., Saraiva,J.P., Zhang,J., Stolte,S., Gillor,O.,
            Harms,H. and Rocha,U.
  TITLE     Genome-centric analyses of 165 metagenomes show that mobile genetic
            elements are crucial for the transmission of antimicrobial
            resistance genes to pathogens in activated sludge and wastewater
  JOURNAL   Microbiol Spectr 12 (3), e0291823 (2024)
   PUBMED   38289113
REFERENCE   2  (bases 1 to 4336)
  AUTHORS   Abdulkadir,N.
  TITLE     Direct Submission
  JOURNAL   Submitted (24-MAR-2023) Environmental Microbiology, Helmholtz
            Center for Environmental Research -UFZ, Permoserstr 15, Leipzig
            04318, Germany
COMMENT     The annotation was added by the NCBI Prokaryotic Genome Annotation
            Pipeline (PGAP). Information about PGAP can be found here:
            https://www.ncbi.nlm.nih.gov/genome/annotation_prok/
            
            ##Genome-Assembly-Data-START##
            Assembly Method        :: SPAdes v. 3.11.1
            Genome Representation  :: Full
            Expected Final Version :: Yes
            Genome Coverage        :: Not Applicable
            Sequencing Technology  :: Illumina MiSeq
            ##Genome-Assembly-Data-END##
            
            ##Genome-Annotation-Data-START##
            Annotation Provider               :: NCBI
            Annotation Date                   :: 05/04/2023 04:57:10
            Annotation Pipeline               :: NCBI Prokaryotic Genome
                                                 Annotation Pipeline (PGAP)
            Annotation Method                 :: Best-placed reference protein
                                                 set; GeneMarkS-2+
            Annotation Software revision      :: 6.5
            Features Annotated                :: Gene; CDS; rRNA; tRNA; ncRNA
            Genes (total)                     :: 2,776
            CDSs (total)                      :: 2,750
            Genes (coding)                    :: 2,714
            CDSs (with protein)               :: 2,714
            Genes (RNA)                       :: 26
            tRNAs                             :: 24
            ncRNAs                            :: 2
            Pseudo Genes (total)              :: 36
            CDSs (without protein)            :: 36
            Pseudo Genes (ambiguous residues) :: 0 of 36
            Pseudo Genes (frameshifted)       :: 2 of 36
            Pseudo Genes (incomplete)         :: 32 of 36
            Pseudo Genes (internal stop)      :: 2 of 36
            ##Genome-Annotation-Data-END##
FEATURES             Location/Qualifiers
     source          1..4336
                     /organism="Micropruina sp."
                     /mol_type="genomic DNA"
                     /submitter_seqid="NODE_8641_length_4336_cov_3.604765"
                     /isolate="AMR_MDS_2195"
                     /isolation_source="Activated sludge of municipal
                     wastewater treatment plants"
                     /db_xref="taxon:2737536"
                     /environmental_sample
                     /geo_loc_name="Canada"
                     /lat_lon="49.25 N 123.1 W"
                     /collection_date="2015-06-16"
                     /metagenome_source="wastewater metagenome"
                     /note="metagenomic"
     gene            <1..514
                     /locus_tag="PLF56_08565"
     CDS             <1..514
                     /locus_tag="PLF56_08565"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_007077384.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=2
                     /transl_table=11
                     /product="tRNA 2-thiouridine(34) synthase MnmA"
                     /protein_id="HOQ53651.1"
                     /translation="ARKPDSHDICFIPSGDTAGFLSRHLGSAEGEFVDERGEVVGAHA
                     GSHRFTVGQRRGLHLGVPADDGRPRYVLGIDPVRNTVTVGPHEAMAVSRIEGIRPSWT
                     EGEVLGPWRGYVQVRAHGAPLPALIEARSDSVAVELDDAAHGIAPGQAVVCYDGDRVV
                     GSATIATTAR"
     gene            514..1497
                     /locus_tag="PLF56_08570"
     CDS             514..1497
                     /locus_tag="PLF56_08570"
                     /inference="COORDINATES: protein motif:HMM:NF013847.2"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="hypothetical protein"
                     /protein_id="HOQ53652.1"
                     /translation="MVGITGVGSLPGTDFAAAVRRVFDTLGEAEHGIPHLPELPARGP
                     WAGMTGRSCALLVDLPVSLDADNWRLSDTAGIDARRARATLRDDLDRLQEDAAEWDGT
                     LKVQVTGPWTLAATVLRPLGGLVLADRSARRDLAQSLAQGTSDFLIEVATRLPRAGLV
                     LQVDEPGLPAVLGGRVASEGGFFRHRSVDRPEAVELLRELSALTESTVLHCCATGLDP
                     NLVRQAGFRGFSLDQGQVHDWDALAEFVDAGHRLWLGCLPTATERDWAPDELVNRVLR
                     VLRPLELGPALADTLVLSPACGLAGWSAVGASGAYRSLVRAAGLVNDALRD"
     gene            complement(1538..1735)
                     /locus_tag="PLF56_08575"
     CDS             complement(1538..1735)
                     /locus_tag="PLF56_08575"
                     /inference="COORDINATES: ab initio
                     prediction:GeneMarkS-2+"
                     /note="Derived by automated computational analysis using
                     gene prediction method: GeneMarkS-2+."
                     /codon_start=1
                     /transl_table=11
                     /product="hypothetical protein"
                     /protein_id="HOQ53653.1"
                     /translation="MTPLHVGYIVAWGGMALAVVVAIAVPVRRMLTNKRVIWSVWPLL
                     GIARVLLAFALGGGIADGVHS"
     gene            1999..3480
                     /locus_tag="PLF56_08580"
     CDS             1999..3480
                     /locus_tag="PLF56_08580"
                     /inference="COORDINATES: protein motif:HMM:NF012234.2"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_function: GO:0005524 - ATP binding [Evidence IEA];
                     GO_function: GO:0016887 - ATP hydrolysis activity
                     [Evidence IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="ATP-binding protein"
                     /protein_id="HOQ53654.1"
                     /translation="MQSWLNLSFDPQPENSEANGADLPALDPVIADALARQLTAVLAM
                     ASRARPSGRSATVAWIEEHLGVSLDEATIVTAEWPTWRHASVQRAVEAFLRENAEWIG
                     LAVPHPEHQGLADLLQQEAASGRRTLARPQLVEVATGPDTMTDALKLGLCRAHSEDGT
                     PIVVCLRLSQEQGNPQAQVDLVLNDPGVGSAVLQRLRELADEHDVLRGQVVRFGASEH
                     YGNHLVSFLPRPAVALEDVVLPDGVLESVTRHLSGIGRRAARLTELGVHLKRGVLLYG
                     PPGTGKTHTARYLVGQALSATVIVLTGQGLSMIEPAAALARRLAPSIVVVEDVDLIAQ
                     DRDYSPTGNPLLFSLLDAMDGVAADADVTFLLTTNRTAELETALVQRPGRVDLAVEIP
                     RPDAAGRERLFGLYARGARLTADLTEAVARTEGVTASAIKELMRRAVLSAVEQGGSTV
                     DGALLGECLTAFFADEARLTRALVGGATTGPAGPVASAALSGE"
     gene            3540..3995
                     /locus_tag="PLF56_08585"
     CDS             3540..3995
                     /locus_tag="PLF56_08585"
                     /inference="COORDINATES: ab initio
                     prediction:GeneMarkS-2+"
                     /note="Derived by automated computational analysis using
                     gene prediction method: GeneMarkS-2+."
                     /codon_start=1
                     /transl_table=11
                     /product="hypothetical protein"
                     /protein_id="HOQ53655.1"
                     /translation="MIIWRGWGALPILIAAAAAGLGALMSMSVPGLRGLFIGLALIGG
                     AVGNWYLGQWLNLEKPRGEFDQWQAGRRSEVAAMVQGGHYSHVPDPQRPGQFADPWAV
                     GEYVLNQESIRVRDGLTNRHSLFFVPMQYFAYLLGIAGIFTIIAFLIPR"
     gene            4052..>4336
                     /locus_tag="PLF56_08590"
     CDS             4052..>4336
                     /locus_tag="PLF56_08590"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_013863842.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="NAD-dependent DNA ligase LigA"
                     /protein_id="HOQ53656.1"
                     /translation="MTSTDDYRTRHAQLAQQIEDHRFRYYVTDAPTISDGDFDALMRE
                     LEAIETEHPELRTPDSPTQKVGGAPSATFAAVTHLQPLMSLDNVFSKEDFE"
BASE COUNT          642 a         1463 c         1568 g          663 t
ORIGIN      
        1 ggccaggaag cccgattcgc acgacatctg tttcattccc tccggcgaca ccgccggctt
       61 tctctcgcgc catctgggct ctgcagaggg cgagttcgtg gacgagcgtg gagaggtggt
      121 cggtgcacat gcgggcagtc accggttcac ggtgggccaa cgccgcggac tacacctggg
      181 tgtgccggcc gacgacggac gtccgcgcta cgtgctcggc atcgacccgg tgcgcaacac
      241 cgtgacggtc ggcccgcacg aggctatggc ggtcagccgg atcgagggca tccggccgtc
      301 ctggaccgag ggcgaagtgc tgggcccgtg gcgtggctac gtgcaggtgc gcgcgcacgg
      361 agcgccgctg ccggccctga tcgaggcccg ctccgattcg gtcgccgtcg agctggacga
      421 cgccgcgcac ggcatagccc caggccaggc tgtggtctgc tacgacggcg atcgggtcgt
      481 cggctcggcc accatcgcca cgacggcacg ctgatggtcg gcatcaccgg ggtcgggtcg
      541 cttcccggta ccgacttcgc cgccgcagtg aggcgggtgt tcgacaccct gggcgaggcc
      601 gagcacggca ttccgcacct gcccgagttg cccgcccgcg ggccgtgggc cgggatgacc
      661 ggacgctcct gcgccctgct ggtcgacctg ccggtgagcc tggacgccga caactggcgg
      721 ctgtcggaca ccgccggcat cgacgcccgc cgagcccggg cgacgctgcg cgacgacctg
      781 gaccggttgc aggaagacgc cgccgagtgg gacggcacgc tgaaggtgca ggtgaccggg
      841 ccatggacac tggccgcaac ggtgctgcgt ccgttgggcg ggttggtgtt agccgatcgc
      901 agcgcccgcc gcgacctggc gcagtcgttg gcacagggca cgtccgactt cttgatcgag
      961 gtggcgacgc gccttccgcg ggcaggattg gtgctgcagg tggacgagcc gggcctgcct
     1021 gcggtgctgg gcggaagagt ggcgagcgag ggtgggtttt ttcggcaccg cagcgtcgat
     1081 cgtccagaag cagtcgagtt gctgcgtgag ttgtcggcgc tgaccgagtc gacggtgctg
     1141 cactgttgcg cgactgggct cgatccgaac ctggtgcggc aggcgggctt ccgcggcttc
     1201 tcgctcgatc agggtcaggt gcatgactgg gacgcgctcg ccgagttcgt cgatgccggc
     1261 catcgactgt ggttgggctg cctgccgacg gccaccgaac gggactgggc gccggacgag
     1321 ctggtcaaca gggtgctgcg cgtgctgcgt ccgctggaac tggggccggc gttggcagac
     1381 accctggtgc tgagcccggc ctgcgggttg gcgggctggt cggccgtggg cgcgtccggc
     1441 gcataccgca gcctggtgcg ggccgctggc ctggtgaacg acgcgttgcg cgactgagtc
     1501 ctatccgggc tgagcccggg cgttcgtccg cggccggcta cgagtggacg ccgtcggcga
     1561 tcccaccgcc gagggcgaac gccagcagca cccgggcgat gccgagcaac ggccacaccg
     1621 accagatcac ccgcttgttc gtcagcatcc gtcgcaccgg cacggcgatc gccaccacga
     1681 ccgcgagcgc catgccgccc caggccacga tgtagccgac gtgcagtggc gtcatgttgc
     1741 atgattcgag ggcccgcatg agtcggtggc catcacgaac atgccggtga acggcagcgc
     1801 gaccaacaac acgaccacca gcaccagata cagcagcccg gccagggatc cagtcgagga
     1861 tcctggccgc cgatccggtg gcgggcgggc tcgactgagg cggtggcggc accgggacgg
     1921 tcatggcgac accgtagcga tacccggtgg caccggctca accaccacat gtgcgacccg
     1981 gcgccctaca ttggggccat gcagagttgg ctcaaccttt ctttcgaccc gcagcccgag
     2041 aactccgagg cgaatggggc tgacctcccg gcactggatc ccgtcatcgc ggacgccctg
     2101 gccaggcagt tgaccgcggt gctggcaatg gcgtcgcgcg cgcgtccgag cggccgatcg
     2161 gccaccgtgg cctggatcga ggagcatctg ggcgtgtcgc tggacgaggc gaccatcgtc
     2221 accgcggagt ggcccacgtg gcggcacgcc tcggtgcagc gggccgtcga ggccttcctc
     2281 cgcgagaacg ccgaatggat cgggctggcc gtgccgcacc ccgaacacca gggtttggcc
     2341 gacctgctgc agcaggaggc ggcatccgga cgtcgcaccc tggcgcgtcc gcagctggtg
     2401 gaggtcgcga ccgggccgga cacgatgacc gacgcgctca aactcggtct atgccgggcg
     2461 cacagcgagg acggcacgcc gatcgtggta tgcctgcggc tgtcccagga gcagggcaat
     2521 ccgcaggcgc aggtcgacct ggtgctcaac gatcccggcg tcggctcggc cgtgctgcag
     2581 cggctgcgcg aactggccga cgagcacgat gtgctgcgcg gccaggtggt gcgcttcggc
     2641 gcaagcgagc actacggcaa ccatctggtc agcttcctac cccgacctgc ggtggccctc
     2701 gaagacgtgg tgttgcccga cggggtgctc gagtcggtga ccaggcacct gagcggcatc
     2761 ggacgacgcg cggcccggct gaccgaactc ggggtgcacc tgaagcgtgg cgtgttgctg
     2821 tacggaccgc caggcaccgg caagacccac accgcgcggt acctggtcgg tcaggctctg
     2881 tcggcgaccg tgatcgtgct gaccggccag gggctgagca tgatcgaacc ggcggcggcg
     2941 ctggcccgcc ggctggcccc gtccatcgtc gtggtcgaag acgtggacct gatcgcgcag
     3001 gatcgcgact acagcccgac cggcaacccg ctgctgttct cgctgctgga cgcgatggac
     3061 ggggtcgccg ccgacgccga cgtcaccttt ctgctcacca ccaaccgcac ggccgaactg
     3121 gaaaccgcac tcgtgcagcg tccgggacgg gtggacctgg ccgtcgagat ccctcgtccg
     3181 gacgccgcgg gccgcgagcg gctgttcggc ttgtacgccc gcggtgcgcg gttgaccgcg
     3241 gacctcaccg aggcggtggc acgcaccgag ggcgtcaccg cgtccgcgat caaggagttg
     3301 atgcgccggg ccgtgctcag cgcggtcgag cagggcggtt cgacggtcga cggcgctctg
     3361 ctcggcgagt gcctgacggc gttcttcgcc gacgaggcgc gtctcacccg cgccctggtc
     3421 gggggtgcga cgacgggtcc cgccggacca gtggcgtccg ctgcgctgag cggtgagtaa
     3481 gggaggccga ggcggacggt gccgagggtc ggcccacgcg acgccgatag tctcggccca
     3541 tgatcatctg gcgtggctgg ggtgcgctgc ccatcctcat cgcggcggcg gccgccggat
     3601 tgggtgcgct gatgtcgatg tccgttccgg gcttgcgggg gctgttcatc ggcctagcgt
     3661 tgatcggcgg cgcagtcggc aactggtact tgggtcagtg gctcaacctt gaaaagcctc
     3721 ggggcgagtt cgaccagtgg caggccgggc ggcgatccga ggtcgccgcg atggtgcagg
     3781 gcgggcacta ctcacatgtg cccgatcccc agcgtccggg ccagttcgcc gacccctggg
     3841 cggtcggtga gtacgtgctg aaccaagaga gcatccgggt gcgcgacggg ttgaccaacc
     3901 ggcactcgtt gttcttcgtg ccgatgcagt acttcgccta tttgttgggc atcgccggca
     3961 tcttcaccat catcgccttc ctgattccgc gctgaacccg ccgcgcgcgg gtgtgcccgg
     4021 aatgtcggag ccgatcagta ggctcaccgg agtgacgagc acagacgact accgcaccag
     4081 gcacgcgcag ctggcccagc agatcgagga ccaccggttc cgctactacg tcaccgacgc
     4141 gcccacgatc agcgacggcg acttcgacgc cctgatgcgc gaactcgagg ccatcgagac
     4201 cgaacacccc gagctgcgta cccccgattc gccgacgcag aaggtgggcg gggcaccgtc
     4261 cgccacgttc gctgccgtca cccacctgca gccgctgatg agcctcgaca acgtgttctc
     4321 gaaggaggac ttcgag
//
LOCUS       DAOSUG010000240         4309 bp    DNA     linear   ENV 10-JUN-2024
DEFINITION  MAG TPA_asm: Micropruina sp. isolate AMR_MDS_2195
            NODE_8687_length_4309_cov_4.764222, whole genome shotgun sequence.
ACCESSION   DAOSUG010000240 DAOSUG010000000
VERSION     DAOSUG010000240.1
DBLINK      BioProject: PRJNA925477
            BioSample: SAMN32864262
            Sequence Read Archive: SRR4236673
KEYWORDS    WGS; ENV; Metagenome Assembled Genome; MAG; Third Party Data; TPA;
            TPA:assembly.
SOURCE      Micropruina sp. (wastewater metagenome)
  ORGANISM  Micropruina sp.
            Bacteria; Bacillati; Actinomycetota; Actinomycetes;
            Propionibacteriales; Nocardioidaceae; Micropruina.
REFERENCE   1  (bases 1 to 4309)
  AUTHORS   Abdulkadir,N., Saraiva,J.P., Zhang,J., Stolte,S., Gillor,O.,
            Harms,H. and Rocha,U.
  TITLE     Genome-centric analyses of 165 metagenomes show that mobile genetic
            elements are crucial for the transmission of antimicrobial
            resistance genes to pathogens in activated sludge and wastewater
  JOURNAL   Microbiol Spectr 12 (3), e0291823 (2024)
   PUBMED   38289113
REFERENCE   2  (bases 1 to 4309)
  AUTHORS   Abdulkadir,N.
  TITLE     Direct Submission
  JOURNAL   Submitted (24-MAR-2023) Environmental Microbiology, Helmholtz
            Center for Environmental Research -UFZ, Permoserstr 15, Leipzig
            04318, Germany
COMMENT     The annotation was added by the NCBI Prokaryotic Genome Annotation
            Pipeline (PGAP). Information about PGAP can be found here:
            https://www.ncbi.nlm.nih.gov/genome/annotation_prok/
            
            ##Genome-Assembly-Data-START##
            Assembly Method        :: SPAdes v. 3.11.1
            Genome Representation  :: Full
            Expected Final Version :: Yes
            Genome Coverage        :: Not Applicable
            Sequencing Technology  :: Illumina MiSeq
            ##Genome-Assembly-Data-END##
            
            ##Genome-Annotation-Data-START##
            Annotation Provider               :: NCBI
            Annotation Date                   :: 05/04/2023 04:57:10
            Annotation Pipeline               :: NCBI Prokaryotic Genome
                                                 Annotation Pipeline (PGAP)
            Annotation Method                 :: Best-placed reference protein
                                                 set; GeneMarkS-2+
            Annotation Software revision      :: 6.5
            Features Annotated                :: Gene; CDS; rRNA; tRNA; ncRNA
            Genes (total)                     :: 2,776
            CDSs (total)                      :: 2,750
            Genes (coding)                    :: 2,714
            CDSs (with protein)               :: 2,714
            Genes (RNA)                       :: 26
            tRNAs                             :: 24
            ncRNAs                            :: 2
            Pseudo Genes (total)              :: 36
            CDSs (without protein)            :: 36
            Pseudo Genes (ambiguous residues) :: 0 of 36
            Pseudo Genes (frameshifted)       :: 2 of 36
            Pseudo Genes (incomplete)         :: 32 of 36
            Pseudo Genes (internal stop)      :: 2 of 36
            ##Genome-Annotation-Data-END##
FEATURES             Location/Qualifiers
     source          1..4309
                     /organism="Micropruina sp."
                     /mol_type="genomic DNA"
                     /submitter_seqid="NODE_8687_length_4309_cov_4.764222"
                     /isolate="AMR_MDS_2195"
                     /isolation_source="Activated sludge of municipal
                     wastewater treatment plants"
                     /db_xref="taxon:2737536"
                     /environmental_sample
                     /geo_loc_name="Canada"
                     /lat_lon="49.25 N 123.1 W"
                     /collection_date="2015-06-16"
                     /metagenome_source="wastewater metagenome"
                     /note="metagenomic"
     gene            complement(80..1084)
                     /gene="gap"
                     /locus_tag="PLF56_08595"
     CDS             complement(80..1084)
                     /gene="gap"
                     /locus_tag="PLF56_08595"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_012920878.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_process: GO:0006094 - gluconeogenesis [Evidence IEA];
                     GO_process: GO:0006096 - glycolytic process [Evidence
                     IEA];
                     GO_process: GO:0019682 - glyceraldehyde-3-phosphate
                     metabolic process [Evidence IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="type I glyceraldehyde-3-phosphate dehydrogenase"
                     /protein_id="HOQ53657.1"
                     /translation="MTVKVGINGFGRIGRNFFRALTASGADIEVVGVNDLTDNKTLAH
                     LLKYDSILHRFPGEISYDDESITVNGHKIMAYEERDPSKLPWGEIGADVVIESTGFFT
                     DAAKAQAHIDAGAKKVIISAPAKNEDITIVIGVNDELYDPAAHHIISNASCTTNCLAP
                     LAKALNDGLGIVKGLMTTIHAYTQDQNLQDAPHKDLRRARAAALNIVPTTTGAAKAVA
                     LVLPELKGKLDGYALRVPVPTGSVTDLTFEASRETSIEEVNAIVKAAAEGPLKGILEY
                     NDDPIVSKDIETYPASSIFDAPLTKVIGNQVKVVSWYDNEWGYSNRLVDLAVLVGSKL
                     "
     gene            complement(1254..2240)
                     /gene="whiA"
                     /locus_tag="PLF56_08600"
     CDS             complement(1254..2240)
                     /gene="whiA"
                     /locus_tag="PLF56_08600"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_013862313.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_function: GO:0003677 - DNA binding [Evidence IEA];
                     GO_process: GO:0043937 - regulation of sporulation
                     [Evidence IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="DNA-binding protein WhiA"
                     /protein_id="HOQ53658.1"
                     /translation="MAMTQQVKAELATVEVTKPACRRAEVAATLRFAGGLHLAGGRIV
                     VEAELDTGGAARRLRTAIADLFGFTSEFVVVTSSGLRRGTRYIVRLIRDGESLARQTG
                     LVDNRGRPVRGLPSGIVSGGLDVATAAWRGAFLAHGSLTEPGRSMALEITCPGSEAAL
                     ALVGAARRLGIAAKAREVRGVDRVVIRDGDAIAAMLTRLGAHESLLAWEERRIRREVR
                     ASANRLANFDDANLRRSARAAVAAGARVERALEILGDDIPEHLKVAGLLRLQNKQASL
                     EELGQLHQPPLTKDAVAGRIRRLLATADKRAAELGIASTEAGLSADMIDDRD"
     gene            complement(2299..3252)
                     /gene="yvcK"
                     /locus_tag="PLF56_08605"
     CDS             complement(2299..3252)
                     /gene="yvcK"
                     /locus_tag="PLF56_08605"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_007077856.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="uridine diphosphate-N-acetylglucosamine-binding
                     protein YvcK"
                     /protein_id="HOQ53659.1"
                     /translation="MKSRRKRPAVVAFGGGHGLYAALSALRYLTTRLTAVVTVADDGG
                     SSGRLRDEFDILPPGDLRMALAALCGDDEVGRTWATVLQSRFGGSGPLAGHAIGNLLI
                     AGLWQQLPDPVAGLDMVGELLRTQGRVLPMAAVPLTIEADVFGADPNHPDELSVLRGQ
                     ATVAKTQGEVLSVRLVPDRPAARPEVVASVQAADHLVLGPGSWFTSVIPHLLVPELAD
                     AIVTSKAQRILTMNLVSDDETHGFQASRHLEMLADHCPRLRLNVVLVDPSFATDDPPL
                     ERYARSLGARLVVENVRMRDGSPRHDPHRLAAVYAELLNRC"
     gene            complement(3249..4121)
                     /gene="rapZ"
                     /locus_tag="PLF56_08610"
     CDS             complement(3249..4121)
                     /gene="rapZ"
                     /locus_tag="PLF56_08610"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_016667355.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_function: GO:0005524 - ATP binding [Evidence IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="RNase adapter RapZ"
                     /protein_id="HOQ53660.1"
                     /translation="MSTSTTQALRFVIVTGMSGAGRRTACHALEDLGWYVVDNLPPQL
                     LPDLAQIAREKQFHRVAVGLDVRTRSAFQQIPAAFDALGRAGVVPQILFLDAADEAIV
                     RRQESVRRPLPLQGEGRLLDGAERERRLLASLRASADLVIDTSGLNVHQLSDRVTHVF
                     GSHDDPELSFTLLSFGFKNGVPIDADMVLDVRFLPNPHWVPDLRPQTGLSQAVSNYVL
                     GRPEATAFLQAATELLTIVKPGYIREGKRLATVGIGCTGGKHRSTAMAIELARRLREN
                     GIATQVLHRDLGKE"
     gene            complement(4129..>4309)
                     /locus_tag="PLF56_08615"
     CDS             complement(4129..>4309)
                     /locus_tag="PLF56_08615"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_013862310.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=2
                     /transl_table=11
                     /product="helix-hairpin-helix domain-containing protein"
                     /protein_id="HOQ53661.1"
                     /translation="KTLLTHFGSMKKLRAASVDELAQVPGIGPRLAAAILEAMAEPVA
                     ETVNLTTGEVLDGAP"
BASE COUNT          689 a         1440 c         1486 g          694 t
ORIGIN      
        1 gcgagaccgc agtgacacgg ggttcgacag gctcaaccaa cgacggaaac gctggtcaac
       61 gcctccttgg tcggaacgat cagagcttgg agccgaccag cacggccagg tccacgaggc
      121 ggttggagta gccccactcg ttgtcgtacc aggagacgac cttgacctgg ttgccgatca
      181 ccttggtgag cggtgcgtcg aagatgctcg aggccgggta ggtctcgatg tccttgctga
      241 cgatcgggtc gtcgttgtac tcaaggatgc ccttgagcgg accctcggcg gcggccttca
      301 cgatggcgtt gacctcttcg atggaggtct cgcgcgaggc ctcgaaggtg aggtcggtga
      361 ccgagccggt cgggaccggg acgcgcagcg cgtagccatc gagcttgccc ttgagttcgg
      421 gcagcaccag cgccacggcc ttggccgcac cggtggtggt cgggacgatg ttgagggcgg
      481 cggcgcgggc gcgacgcagg tccttgtgcg gagcgtcctg caggttctgg tcctgggtgt
      541 aggcgtgaat ggtggtcatc aggcccttga cgatgcccag gccgtcattc agcgccttgg
      601 ccaggggggc caggcagttg gtggtgcagg aagcgttgct gatgatgtgg tgtgcggccg
      661 ggtcgtagag ctcgtcgttg acgccgatca cgatggtgat gtcttcgttc ttggccggag
      721 ccgagatgat caccttcttg gcgccggcat cgatgtgtgc ctgcgccttg gcggcatcgg
      781 tgaagaagcc ggtggactcg atcaccacgt cggcgccgat ctcgccccag ggcagcttgc
      841 tcgggtcgcg ctcttcgtac gccatgatct tgtgcccgtt gaccgtgatc gactcgtcgt
      901 cgtagctgat ctcgcccggg aagcggtgca ggatcgagtc gtacttgagc aggtgcgcga
      961 gcgtcttgtt gtcggtcagg tcattgacgc caacgacctc gatgtcggca ccggacgcag
     1021 tcagcgcgcg gaagaagttg cgaccgatcc ggccgaagcc gttgatacca accttcaccg
     1081 tcatgtgatg gttccccttc attgagtttt tgcccgcgct gaggcgtagg caagggtgga
     1141 catgactcac tctatagaca aagcgggggg cgtgaaccct ctatcgaaag ccggttccgc
     1201 gaactggtca attggcgctg aacacaggtg atagcgtgtt gagtcggcca ccttcagtcg
     1261 cgatcgtcga tcatgtcggc cgagaggccg gcctcggtcg acgcaatgcc cagttctgct
     1321 gcccgcttgt ctgcggtggc cagcagccgt cggatccggc ccgccaccgc gtctttggtc
     1381 agcggtggct gatgcagttg ccccaactct tccagtgagg cctgcttgtt ctgcagccgc
     1441 agcagccccg ccaccttgag atgctccggt atgtcgtcgc ccagaatctc gagcgcacgt
     1501 tccacccggg ccccggcggc gacggccgca cgtgccgagc ggcgcaggtt ggcgtcgtcg
     1561 aagttcgcca ggcggttggc cgaggcccgc acctcgcggc ggatgcggcg ctcctcccag
     1621 gccagcagcg actcgtgagc gcccagccgg gtgagcatgg cggcgatcgc gtcgccgtcg
     1681 cggatcacca ctcggtcgac gccgcgcacc tcgcgcgctt tcgcggcgat gccgagccgc
     1741 cgggcggcac cgaccagcgc cagtgcggcc tccgagccgg ggcaggtgat ctcgagcgcc
     1801 atcgaccggc ccggctcggt cagcgagccg tgggccagga acgccccccg ccaggccgcg
     1861 gtcgccacgt ccagtccccc gctcacgatg ccggacggaa gcccgcgcac cgggcgtcca
     1921 cggttgtcca ccaggcccgt ctggcgggcc agcgactccc cgtcgcggat cagccggacg
     1981 atatagcggg tgccccggcg caggccggac gaggtcacga caacgaactc cgaggtgaac
     2041 ccaaacaggt cggcgatcgc cgtgcgcagc cggcgggcgg caccgccggt gtcgagttcg
     2101 gcctcgacca cgatgcgtcc gccggccagg tgcaggccgc ccgcgaagcg cagcgtggcc
     2161 gccacctcgg cgcgccgaca ggccggcttg gtcacctcaa cggtggccaa ctctgccttg
     2221 acctgctggg tcatcgccat ggatacatcc ttacctcgag ggtgtgcttt cgtggccgaa
     2281 caccgaacct agtcgctgtc aacagcggtt caacagttcg gcgtagacgg ccgccaggcg
     2341 atgggggtca tgtcggggcg agccgtcgcg catgcgcaca ttctctacca ccaggcgggc
     2401 tcccagcgaa cgcgcgtaac gctccagcgg cggatcgtcc gtcgcgaagc tcggatcgac
     2461 cagcaccaca ttgagccgta accgcgggca gtggtcggcc agcatctcga gatggcggga
     2521 cgcctgaaag ccgtgggtct cgtcgtcgga caccaggttc atggtcagga tccgctgcgc
     2581 cttcgaggtc acgatcgcgt cggccaactc gggtaccagc agatgaggga tgaccgaggt
     2641 gaaccacgag ccgggaccca acaccagatg gtcggcagcc tgcacgctcg ccaccacctc
     2701 gggacgtgcc gccggacgat caggcacgag ccggacgctg agcacctcgc cctgggtctt
     2761 cgccaccgtt gcctgcccgc gcagcaccga aagctcgtcg ggatggttcg ggtcagcgcc
     2821 gaacacgtcc gcctcaatgg tcagcggcac cgcggccatc ggtagcaccc ggccctgcgt
     2881 gcgcagcagc tcacccacca tgtcgagccc ggccaccgga tcgggcagtt gctgccacag
     2941 gccggcgatc agcaggttgc cgatcgcatg gccggccaac gggcccgacc cgccgaagcg
     3001 cgactgcaac accgtcgccc aggtccggcc gacctcgtcg tcaccgcaca gggcggccag
     3061 cgccatgcgg agatcgccgg gcggcagaat gtcgaactcg tcgcgaagcc gtccggacga
     3121 gccgccgtcg tcggccaccg tgaccacggc ggtcaaccgg gtggtcaggt agcgcagcgc
     3181 actgagcgct gcgtacagcc cgtgaccgcc gccgaacgcg accacggccg gacgtttgcg
     3241 cctcgacttc actccttgcc cagatcccgg tgcagcacct gggtggcgat gccgttctcg
     3301 cgcaaccgcc gagccaactc gatcgccatc gcggtgctgc ggtgcttgcc gccggtgcag
     3361 ccgatgccga cggtggccaa ccgcttgccc tcgcggatgt agccgggctt gacgatggtg
     3421 agcagttcgg tcgcggcctg cagaaaggcg gtcgcctcgg gacgtccgag cacgtagttc
     3481 gagacggcct gcgacaggcc ggtctggggt cgcagatcgg gcacccagtg cgggttgggc
     3541 agaaagcgca cgtcgagcac catgtcggcg tcgatcggca cgccgttctt gaacccgaac
     3601 gacagcagcg tgaacgagag ctcggggtcg tcgtgcgagc cgaacacgtg cgtcacccga
     3661 tcggacaact ggtgcacgtt gagtccggag gtgtcgatca ccagatcggc cgaggcgcgc
     3721 agcgacgcca gcagccggcg ttcgcgttcg gcgccgtcca gcaggcggcc ctcaccctgc
     3781 agcggcaggg gtcggcgcac cgactcctgc cggcgcacga tggcctcgtc ggcggcgtcc
     3841 agaaacagga tctgcggcac cacaccggcc cgtccgaggg cgtcgaaggc cgcgggaatc
     3901 tgctggaacg ccgagcgggt gcgcacgtca aggcccacgg ccacccgatg gaactgcttc
     3961 tcgcgcgcga tctgcgccag atcgggcagc agttgcggcg gcaggttgtc gaccacgtac
     4021 cagcccaggt cttcaagcgc atggcaggcg gtgcgccggc ccgcgccgga catgccggtg
     4081 acgatcacga aacgcagagc ctgggtggtg ctggtgctca tgaagtcatt atggtgcgcc
     4141 gtcgagcacc tcgccggtgg tcaggttgac cgtctcggcg acgggctcgg ccatggcctc
     4201 gagaatcgcc gcggccaagc ggggcccgat gccgggaacc tgcgccagtt cgtccactga
     4261 ggcggcgcgc agcttcttca tcgagccgaa gtgtgtgagc aacgtcttg
//
LOCUS       DAOSUG010000241         4294 bp    DNA     linear   ENV 10-JUN-2024
DEFINITION  MAG TPA_asm: Micropruina sp. isolate AMR_MDS_2195
            NODE_8736_length_4294_cov_4.163010, whole genome shotgun sequence.
ACCESSION   DAOSUG010000241 DAOSUG010000000
VERSION     DAOSUG010000241.1
DBLINK      BioProject: PRJNA925477
            BioSample: SAMN32864262
            Sequence Read Archive: SRR4236673
KEYWORDS    WGS; ENV; Metagenome Assembled Genome; MAG; Third Party Data; TPA;
            TPA:assembly.
SOURCE      Micropruina sp. (wastewater metagenome)
  ORGANISM  Micropruina sp.
            Bacteria; Bacillati; Actinomycetota; Actinomycetes;
            Propionibacteriales; Nocardioidaceae; Micropruina.
REFERENCE   1  (bases 1 to 4294)
  AUTHORS   Abdulkadir,N., Saraiva,J.P., Zhang,J., Stolte,S., Gillor,O.,
            Harms,H. and Rocha,U.
  TITLE     Genome-centric analyses of 165 metagenomes show that mobile genetic
            elements are crucial for the transmission of antimicrobial
            resistance genes to pathogens in activated sludge and wastewater
  JOURNAL   Microbiol Spectr 12 (3), e0291823 (2024)
   PUBMED   38289113
REFERENCE   2  (bases 1 to 4294)
  AUTHORS   Abdulkadir,N.
  TITLE     Direct Submission
  JOURNAL   Submitted (24-MAR-2023) Environmental Microbiology, Helmholtz
            Center for Environmental Research -UFZ, Permoserstr 15, Leipzig
            04318, Germany
COMMENT     The annotation was added by the NCBI Prokaryotic Genome Annotation
            Pipeline (PGAP). Information about PGAP can be found here:
            https://www.ncbi.nlm.nih.gov/genome/annotation_prok/
            
            ##Genome-Assembly-Data-START##
            Assembly Method        :: SPAdes v. 3.11.1
            Genome Representation  :: Full
            Expected Final Version :: Yes
            Genome Coverage        :: Not Applicable
            Sequencing Technology  :: Illumina MiSeq
            ##Genome-Assembly-Data-END##
            
            ##Genome-Annotation-Data-START##
            Annotation Provider               :: NCBI
            Annotation Date                   :: 05/04/2023 04:57:10
            Annotation Pipeline               :: NCBI Prokaryotic Genome
                                                 Annotation Pipeline (PGAP)
            Annotation Method                 :: Best-placed reference protein
                                                 set; GeneMarkS-2+
            Annotation Software revision      :: 6.5
            Features Annotated                :: Gene; CDS; rRNA; tRNA; ncRNA
            Genes (total)                     :: 2,776
            CDSs (total)                      :: 2,750
            Genes (coding)                    :: 2,714
            CDSs (with protein)               :: 2,714
            Genes (RNA)                       :: 26
            tRNAs                             :: 24
            ncRNAs                            :: 2
            Pseudo Genes (total)              :: 36
            CDSs (without protein)            :: 36
            Pseudo Genes (ambiguous residues) :: 0 of 36
            Pseudo Genes (frameshifted)       :: 2 of 36
            Pseudo Genes (incomplete)         :: 32 of 36
            Pseudo Genes (internal stop)      :: 2 of 36
            ##Genome-Annotation-Data-END##
FEATURES             Location/Qualifiers
     source          1..4294
                     /organism="Micropruina sp."
                     /mol_type="genomic DNA"
                     /submitter_seqid="NODE_8736_length_4294_cov_4.163010"
                     /isolate="AMR_MDS_2195"
                     /isolation_source="Activated sludge of municipal
                     wastewater treatment plants"
                     /db_xref="taxon:2737536"
                     /environmental_sample
                     /geo_loc_name="Canada"
                     /lat_lon="49.25 N 123.1 W"
                     /collection_date="2015-06-16"
                     /metagenome_source="wastewater metagenome"
                     /note="metagenomic"
     gene            complement(<1..458)
                     /locus_tag="PLF56_08620"
     CDS             complement(<1..458)
                     /locus_tag="PLF56_08620"
                     /inference="COORDINATES: protein motif:HMM:NF012312.2"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_component: GO:0016020 - membrane [Evidence IEA];
                     GO_function: GO:0022857 - transmembrane transporter
                     activity [Evidence IEA];
                     GO_process: GO:0055085 - transmembrane transport [Evidence
                     IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="MFS transporter"
                     /protein_id="HOQ53662.1"
                     /translation="MTASPQQAATASYTKQVTDKEVRRATVAGAVGSFVEWYDYGIYG
                     LLATYLALNLMGGAAAGALFLTNVGFLVSFVARPFGSVICGYLGDRMGRKTLLAVLLI
                     LIAGATTAIGLIPSYTVIGIAAPILLVSLRVLQGFSAGGEVAGAMSFVGE"
     gene            698..1333
                     /locus_tag="PLF56_08625"
     CDS             698..1333
                     /locus_tag="PLF56_08625"
                     /inference="COORDINATES: protein motif:HMM:NF015682.2"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="methyltransferase"
                     /protein_id="HOQ53663.1"
                     /translation="MSGIIRSSEWQRPDPQLVRQFAEFPVAVIGDSMERLDIIDGGIS
                     PIWTGARCVGSALPIYTTAGDNLAIIEALALIREGDVVCVNAGGYPGRALMGDNLAQR
                     FDVFGAVGAVVDGYVRDRDVIAELGFPVFARGLTPAGPWKNGPGRIGEPIALGGVVVN
                     PGDIVAADADGIAVIRPGLAEAILANCRAMAQIEAEKDAEVLELRAARMAR"
     gene            1404..2030
                     /locus_tag="PLF56_08630"
     CDS             1404..2030
                     /locus_tag="PLF56_08630"
                     /inference="COORDINATES: protein motif:HMM:NF012655.2"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_function: GO:0003677 - DNA binding [Evidence IEA];
                     GO_function: GO:0003700 - DNA-binding transcription factor
                     activity [Evidence IEA];
                     GO_process: GO:0006355 - regulation of DNA-templated
                     transcription [Evidence IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="TetR/AcrR family transcriptional regulator"
                     /protein_id="HOQ53664.1"
                     /translation="MPKAIRSDTARNRRQIIKAAADLVAAEGPNVRMSDVAERAEVSL
                     ATAYRHFSSVDEALVQYRMDAGLKLYAHSVSSEARGLELLKDVSDYWITLVLRHGRVM
                     VTTRSREGFLKRLRSGARYLTVAADALDEPIRQAAAELGLPDPGDEGMFLWNMMFDPR
                     EIFDMVETLGLTQEQVGQRLVGAFCGALQGWIHASSSRSQPEATTQAS"
     gene            2282..3271
                     /gene="holA"
                     /locus_tag="PLF56_08635"
     CDS             2282..3271
                     /gene="holA"
                     /locus_tag="PLF56_08635"
                     /EC_number="2.7.7.7"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_013864326.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_component: GO:0009360 - DNA polymerase III complex
                     [Evidence IEA];
                     GO_function: GO:0003887 - DNA-directed DNA polymerase
                     activity [Evidence IEA];
                     GO_process: GO:0006260 - DNA replication [Evidence IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="DNA polymerase III subunit delta"
                     /protein_id="HOQ53665.1"
                     /translation="MTAPFATATLVQGPESLLAERAVQSLVAQALERRADATVSRLSA
                     GGLDRGTLAECTAGSLFSPAAVAVIEQLGDLPSELADDLVALAQQPGDDLALIMVHEG
                     GAKGRALLERLRRADVTLVEASTVKTWELPQFVMAEVRKQQGRIDRGTAEALVSAVGS
                     DLRSVAAAVTQLLADTEHATITAADVQRYFAGRAEVSGFAIADHCLDGNREAALSSLR
                     WALETGVAPVLLTSAVASGLRNLGKYLGARDDRLRDIELAKRIGVPPWKLKDLSRQSR
                     GWTSAGVAQAIRHTATADASVKGASGDAQYALEVLVLTVIESRGRPPQGTPGR"
     gene            complement(3682..3942)
                     /gene="rpsT"
                     /locus_tag="PLF56_08640"
     CDS             complement(3682..3942)
                     /gene="rpsT"
                     /locus_tag="PLF56_08640"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_012920011.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_component: GO:0000312 - plastid small ribosomal subunit
                     [Evidence IEA];
                     GO_component: GO:0022627 - cytosolic small ribosomal
                     subunit [Evidence IEA];
                     GO_function: GO:0003735 - structural constituent of
                     ribosome [Evidence IEA];
                     GO_process: GO:0006412 - translation [Evidence IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="30S ribosomal protein S20"
                     /protein_id="HOQ53666.1"
                     /translation="MANIKSQMKRIKTNEKARQRNKAVKSALRTHVRRFREAADEGNA
                     EKAQTLASTACRALDKAASKGVIHSNQAANRKSAIAAKAASL"
BASE COUNT          755 a         1359 c         1462 g          718 t
ORIGIN      
        1 tactcaccga cgaatgacat ggcacccgcc acctcacccc ctgccgagaa gccctgcagg
       61 acccgcaacg acacgagcag gatgggggcg gcgatgccga tcacggtata gctggggatc
      121 aggccgatgg cggtggtggc gcctgcgatc aggatcagca gcaccgccag cagggtcttg
      181 cggcccatgc ggtcgcccag gtaaccacag atcacactgc cgaaggggcg cgcgacgaag
      241 ctgaccagga agcccacgtt ggtgaggaac agggcgccgg ctgccgcccc gcccatcagg
      301 ttcagcgcga ggtaggtggc caataggccg tagatgccgt agtcgtacca ctcgacgaag
      361 gaacctacgg cgcccgccac ggtggcacgt cggacttcct tgtcggtgac ctgcttggtg
      421 tagctggcgg tggccgcctg ctgtggtgat gcagtcatga tgtcctcgct gggttcggga
      481 gttggagact cactttcggc ggctttcgtc ggcgcctcga tatgagaatg catcttactt
      541 cagcagttcg tcaagagggt ttcgcactct gtaacgcgct agtcacatgc agatccatag
      601 cgatcgtcat gcatctgacg ctcttagcac cttgcgtctg caggaggctt gagccaaact
      661 tgaagtgaca aatgattctc aaatgagagg cagttcgatg agcggcatca tccgaagcag
      721 tgagtggcag cggcccgatc cgcagttggt caggcagttc gcagagttcc cggtggctgt
      781 gatcggagac tccatggagc gtctggacat catcgacggc ggcatttccc caatctggac
      841 cggcgcccgt tgcgtcggct cggcgttgcc catctacacc accgccggcg acaaccttgc
      901 aatcatcgag gcgctcgcgc tgatccggga aggcgatgtc gtgtgcgtca acgccggtgg
      961 gtacccggga agggcgctga tgggcgacaa ccttgcacag cgattcgacg tcttcggtgc
     1021 cgtcggggcg gtagtcgacg gctacgtccg cgaccgcgat gtaattgcag agttgggctt
     1081 cccggtcttc gcccgcggcc tgacgccggc cggaccatgg aagaacggcc ccggccggat
     1141 cggcgaaccg atcgcactgg gtggcgtcgt ggtgaacccc ggagacattg tggcggccga
     1201 cgccgacggc atcgctgtga ttcggccagg gctggccgaa gcgatcctcg cgaactgccg
     1261 tgcaatggcg cagatcgaag cggagaagga cgccgaagtg ctcgagttgc gagccgcacg
     1321 catggcccgg tgagcgcgca tcactcacag acgctatcct cgggccagtc cctgggatgg
     1381 tcggcagccc agaaggagca acgatgccaa aggcgatacg cagcgacact gcgcgaaacc
     1441 ggcgacagat catcaaagcc gccgccgatc tggtggcggc cgaaggtccc aatgtgcgca
     1501 tgagcgatgt cgcagaacgc gccgaagtct cactcgccac ggcctatcgc cacttcagct
     1561 cggtggacga agcacttgtg caataccgca tggacgcagg tctgaaactc tacgcacaca
     1621 gcgtctcctc agaagcgcgc ggactcgagt tgctcaaaga cgtcagtgac tactggatca
     1681 ctctcgtgct gcgtcacggt cgtgtcatgg tcacgacccg gtcacgcgag ggattcctca
     1741 agcggctccg atcaggagcc cgctacctca ccgtggctgc cgacgctctc gatgaaccaa
     1801 tccgtcaagc ggccgccgaa ctcggcctgc ccgaccccgg cgatgagggc atgttcctgt
     1861 ggaacatgat gttcgaccca cgcgaaatct tcgacatggt cgagacgctg ggactcaccc
     1921 aggagcaagt cgggcagcga ttggtcgggg ccttctgcgg cgcacttcaa ggctggatcc
     1981 acgccagcag cagccgctcc cagccagagg caacgacaca agcaagctag gataggagcg
     2041 gctgcgaaac gctgaacggt cgctggtcac gctcgtccag acccgtgcga ttgaggggct
     2101 tcgacaagct caatcaacgg attgctcgtc gatcggcgtc tcccgatatc gtgcgaggcc
     2161 ttgcccacac catggctggt tgcccggcct ctgtcgacct ggcgtgtgac gactctgacc
     2221 tgagccgcgt gcgaggcgtc cagcgtcact gcgtgtgggt gccgactggc atgctgaggg
     2281 tgtgaccgca ccgttcgcca ccgccacgct cgtccagggc ccggaatcgc tgctggccga
     2341 acgtgcggtc cagtcgctgg tggcgcaggc ccttgagcgg cgtgcggacg ccaccgtgtc
     2401 gcgactgagc gccggtggac tcgatcgggg cacgttggcc gagtgcacgg ccggatcctt
     2461 gttctcgccg gcggcggtcg cagtcatcga gcaactgggc gacctgccgt ccgaactggc
     2521 cgacgacctg gtggcactcg cgcagcagcc gggtgacgat ctggcgttga tcatggtgca
     2581 tgagggcggt gcgaagggcc gggcgttgct cgaacggctg cgcagggccg acgtcacgct
     2641 ggtcgaggcg tccaccgtga agacctggga gttgccgcag ttcgtgatgg ccgaggtgcg
     2701 caagcagcag ggccgcatcg accgcggcac ggcagaggca ttggtgtcgg cggtgggcag
     2761 cgacctgcgc agcgtcgccg ccgcggtcac ccaattgctc gccgacaccg agcatgccac
     2821 catcaccgcc gcggacgtac agcgctactt cgccggacgt gccgaggtca gcggtttcgc
     2881 catcgccgac cactgcctcg acggcaacag ggaggcggcg ctgagcagcc tgcgctgggc
     2941 gttggaaacc ggggtggcgc ccgtactgct gaccagtgcg gtggcatcgg gcctgcgcaa
     3001 cctgggtaag tacctcggcg cgcgcgatga tcggcttcgc gacatcgagt tggccaagcg
     3061 gatcggcgtg ccgccttgga agctgaaaga cctcagccgc cagtcgcgag gctggacgag
     3121 tgccggcgtt gcgcaggcca tccggcacac cgccacggcc gacgcctcag tcaagggcgc
     3181 ctcaggtgat gcccaatacg ccctcgaagt gctcgtgctg accgtcatcg agtcccgcgg
     3241 cagaccgcca caggggacgc ccggtagatg acgtccagta gctagcgcca ggggagcgga
     3301 gcgatcgctg aggggtgcga gcacttggtt gagtccgccc ggacgggcga aacccgccct
     3361 gcggtggggg gagtaccgcg ggcgggttcc gcgctcgtgg ggggcctcag cgcaccgtgc
     3421 gctcggccaa cgggtacggc ggggtggcgg cgcgcgccgt ccgatgctgg cacagccaac
     3481 catacgtcgc gagtcgtcgg caacttgggg gaggagggcc cccgatggct ggcacgagcg
     3541 gccatcgaac agtggcgccg aggttgatca gtgcgcgcac gctgatagct gtccagggtg
     3601 ccggcgtcga gctgagcacg cgaaaacgcc gctcgaccag gtcgagcggc gcagcgtcgg
     3661 atcagctgcc gacgcgttcg ctcagagcga ggcggccttg gcggcgattg ccgacttgcg
     3721 gttggcggcc tggttgctgt ggatgacgcc cttggacgcg gccttgtcga gtgcgcggca
     3781 cgcggtgctg gccagggttt gcgccttctc ggcgttgccc tcgtctgcgg cctcgcggaa
     3841 ccggcgcacg tgggtacgca gcgccgactt cacggccttg ttgcgctgac gagccttctc
     3901 gttcgtcttg atgcgcttca tctgggactt gatgttcgcc acagggcttg cctcttcggt
     3961 acttgatact gggtgcgcgt ccagacgcgg acgcgaccac caaccctacc aaagctgcca
     4021 gcagggcacc aaaacacgcg acctcggacc agaaccacga gttctggctg tcgcagcggg
     4081 gagataacgc cggtagggga cccgccgcca ggtggtcact gaccgcaccc gcgcggcaca
     4141 acgaccgagt tggaccacca cttcaactgt tctgaacagg tcgctgttgt gagattgtgt
     4201 cggcatcgtc gtctgggacg ctcaattcgt ccggtgaccc aagggtggcg gtgcagccga
     4261 ctttcatgct ggacccgaag cggaaggccc ccaa
//
LOCUS       DAOSUG010000242         4290 bp    DNA     linear   ENV 10-JUN-2024
DEFINITION  MAG TPA_asm: Micropruina sp. isolate AMR_MDS_2195
            NODE_8748_length_4290_cov_3.693979, whole genome shotgun sequence.
ACCESSION   DAOSUG010000242 DAOSUG010000000
VERSION     DAOSUG010000242.1
DBLINK      BioProject: PRJNA925477
            BioSample: SAMN32864262
            Sequence Read Archive: SRR4236673
KEYWORDS    WGS; ENV; Metagenome Assembled Genome; MAG; Third Party Data; TPA;
            TPA:assembly.
SOURCE      Micropruina sp. (wastewater metagenome)
  ORGANISM  Micropruina sp.
            Bacteria; Bacillati; Actinomycetota; Actinomycetes;
            Propionibacteriales; Nocardioidaceae; Micropruina.
REFERENCE   1  (bases 1 to 4290)
  AUTHORS   Abdulkadir,N., Saraiva,J.P., Zhang,J., Stolte,S., Gillor,O.,
            Harms,H. and Rocha,U.
  TITLE     Genome-centric analyses of 165 metagenomes show that mobile genetic
            elements are crucial for the transmission of antimicrobial
            resistance genes to pathogens in activated sludge and wastewater
  JOURNAL   Microbiol Spectr 12 (3), e0291823 (2024)
   PUBMED   38289113
REFERENCE   2  (bases 1 to 4290)
  AUTHORS   Abdulkadir,N.
  TITLE     Direct Submission
  JOURNAL   Submitted (24-MAR-2023) Environmental Microbiology, Helmholtz
            Center for Environmental Research -UFZ, Permoserstr 15, Leipzig
            04318, Germany
COMMENT     The annotation was added by the NCBI Prokaryotic Genome Annotation
            Pipeline (PGAP). Information about PGAP can be found here:
            https://www.ncbi.nlm.nih.gov/genome/annotation_prok/
            
            ##Genome-Assembly-Data-START##
            Assembly Method        :: SPAdes v. 3.11.1
            Genome Representation  :: Full
            Expected Final Version :: Yes
            Genome Coverage        :: Not Applicable
            Sequencing Technology  :: Illumina MiSeq
            ##Genome-Assembly-Data-END##
            
            ##Genome-Annotation-Data-START##
            Annotation Provider               :: NCBI
            Annotation Date                   :: 05/04/2023 04:57:10
            Annotation Pipeline               :: NCBI Prokaryotic Genome
                                                 Annotation Pipeline (PGAP)
            Annotation Method                 :: Best-placed reference protein
                                                 set; GeneMarkS-2+
            Annotation Software revision      :: 6.5
            Features Annotated                :: Gene; CDS; rRNA; tRNA; ncRNA
            Genes (total)                     :: 2,776
            CDSs (total)                      :: 2,750
            Genes (coding)                    :: 2,714
            CDSs (with protein)               :: 2,714
            Genes (RNA)                       :: 26
            tRNAs                             :: 24
            ncRNAs                            :: 2
            Pseudo Genes (total)              :: 36
            CDSs (without protein)            :: 36
            Pseudo Genes (ambiguous residues) :: 0 of 36
            Pseudo Genes (frameshifted)       :: 2 of 36
            Pseudo Genes (incomplete)         :: 32 of 36
            Pseudo Genes (internal stop)      :: 2 of 36
            ##Genome-Annotation-Data-END##
FEATURES             Location/Qualifiers
     source          1..4290
                     /organism="Micropruina sp."
                     /mol_type="genomic DNA"
                     /submitter_seqid="NODE_8748_length_4290_cov_3.693979"
                     /isolate="AMR_MDS_2195"
                     /isolation_source="Activated sludge of municipal
                     wastewater treatment plants"
                     /db_xref="taxon:2737536"
                     /environmental_sample
                     /geo_loc_name="Canada"
                     /lat_lon="49.25 N 123.1 W"
                     /collection_date="2015-06-16"
                     /metagenome_source="wastewater metagenome"
                     /note="metagenomic"
     gene            complement(668..1288)
                     /locus_tag="PLF56_08645"
     CDS             complement(668..1288)
                     /locus_tag="PLF56_08645"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_013864635.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="DUF305 domain-containing protein"
                     /protein_id="HOQ53667.1"
                     /translation="MRLTLPAAAVATVIGLAGCSPNDTANMPGTASAPTSTSPSTTGA
                     TPSATPAAGPHNDADVMFAAMMIPHHQQAVEMADLVLATEGINIEVTGLASQIKAAQA
                     PEIAQMSGWLAGWGENRSPSMPMDHDMGGGMMSRADMEALDRATGDEAARLFLAGMIE
                     HHNGAIAMAKQEATNGQNPDAKQLAQDIIDAQQAEIVTMNRLLDQL"
     gene            complement(1436..2167)
                     /locus_tag="PLF56_08650"
     CDS             complement(1436..2167)
                     /locus_tag="PLF56_08650"
                     /inference="COORDINATES: ab initio
                     prediction:GeneMarkS-2+"
                     /note="Derived by automated computational analysis using
                     gene prediction method: GeneMarkS-2+."
                     /codon_start=1
                     /transl_table=11
                     /product="hypothetical protein"
                     /protein_id="HOQ53668.1"
                     /translation="MKLAGPPDDKEVDVEEWREVAALSTTFLWDSWSDPRIVPCCYAI
                     RIDIWDRALSSNHWGGGHAWPGLLPGVVACGGDIPVNAGYGLDMVRIGVAVGARQRLW
                     LLLGVVAMALSLLAMHQLSSNHTAAGHPASPSSTDATLLMSAVHAESHEDAVGGDHAH
                     LTPSTGVGHPVSEVGGCLGCADHSEMGLTCLAALILLMSGWVLTRPGAGPGVWVRRLL
                     HLRLAQRNSWLPQPRSLAQLSVSRT"
     gene            2488..>4290
                     /locus_tag="PLF56_08655"
     CDS             2488..>4290
                     /locus_tag="PLF56_08655"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_008359882.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_function: GO:0042625 - ATPase-coupled ion transmembrane
                     transporter activity [Evidence IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="heavy metal translocating P-type ATPase"
                     /protein_id="HOQ53669.1"
                     /translation="MNKHENGHTSTPSRVKDPVCGMDVDPTTSEHHLVHDGTTYYFCS
                     GQCRNKFDLAPDAYLSPATGHPTGHGHGGNAATPPPGTEWTCPMHPEILRPGPGSCPI
                     CGMALEPVMVTADSGPSPELADMTRRFWVGVALSIPVVILGMGGDLVPAIHDAISPRA
                     SAWVQLVLATPVVLWAGWPFFVRGWTSVRTLKLNMFTLIAMGTGVAWLFSVVATLAPD
                     LFPEAFRMDGAVDVYFEAASVITTLVLLGQVLELRAREQTSGAIRALLDLTPDTAHRL
                     KSDGTEEEVTLDQVTVGDRFRVRPGEKVPVDGDVEEGRSTLDESLVTGESMPATKKVG
                     DSVIGGTINQTGSLIVRADKVGRDTMLARIVQMVAEAQRSRAPIQRVADRVAAWFVPA
                     VIAIAIVAFLVWATAGPDPRLAHALVVAVSVLIIACPCALGLATPMSIMVGVGRGAGL
                     GVLIKNAEALERMEKVDTLVVDKTGTLTEGKPSVTRVVTTTGFEEHEILQLVAAVEQA
                     SEHPLGLAIVNAAHAAGLSIPNVTDCDAPVGKGVVGTVEDQRIRVGSATFLTGEGIDP
                     SALTAQADELRADGATVIFVSSGDQVAAIIAIADP"
BASE COUNT          718 a         1390 c         1441 g          741 t
ORIGIN      
        1 tgttcccgtc taggcagtgc gtgaccggcc aggatggctt cgtacgcgtc gcgtgcgtct
       61 tcgtcgacga ttggctgctg tgatgagtat cggccgtcgg tccggtcacg cttgggaccg
      121 gacacccagt agtggtcgcc gctctcgatg tcggaaaagt tcgcgtcggc attggcgaac
      181 tttgtgcccg tgacccgccg cagccgcttc ccatgaaacg tcagagttcg ccatgacctg
      241 ctaaacgaac acgcgaaatc caggcaggcc cgcggtcaac gtcttgaccc gtcttcaact
      301 gcacgtacat gatgcgctcg gccacggcgg catcgtaatc aatcgcgtcg gtgctcgacc
      361 gggctgaggg gtgagagtcc cgtggagtgg atgccaaccc gacatgcggc tgcggtcctg
      421 gacgttgacg taggtgtagt ggcggatgta cccggaccgg caggcgttga tgcaaacgag
      481 cagcagagcg cgggtgatgc ttgcgtcgta gccggcctgt cgagacagga ctcgcgccgt
      541 ggtggcgcaa atgtcggcgc agtccaggtc ggcgctgatg cagctacgga cttgggtgac
      601 catcggcgag gtcggtggtc tccaggcgcc accggcccgg aaggggacgg tggccgctgg
      661 aaacggatca gagctggtcg agtagccggt tcatggtgac gatctcggcc tgctgagcgt
      721 cgatgatgtc ttgggcgagc tgcttggcgt ccgggttctg gccgttggtg gcctcttgct
      781 tcgccatggc gatggcgcca ttgtggtgct cgatcatccc cgcgaggaac aggcgggcgg
      841 cctcgtcacc ggtggccctg tcgagggcct ccatgtcggc ccggctcatc atgcctccgc
      901 ccatgtcgtg gtccatcggc atcgacgggc tccggttctc gccccagccg gcgagccagc
      961 cgctcatctg ggcgatctcg ggagcctggg cggccttgat ctgggaggca agacctgtga
     1021 cctcgatgtt gatgccttct gtggccaaga cgaggtcggc catttcgacg gcctgctgat
     1081 ggtgcgggat catcatcgcc gcgaacatga cgtccgcgtc gttgtgcgga ccggcggccg
     1141 gggtggccga cggcgtcgcg cccgtcgtcg atggcgacgt cgaggtcggg gcgctggcgg
     1201 tgccgggcat gttcgccgtg tcgttgggcg agcagccggc gaggccgatc accgtggcaa
     1261 ccgcggccgc gggcagggtc aggcgcaaaa aattgaatga gtttacaggc atggcaggca
     1321 tatccttctg ggtagctact gaggaatagg acggaacacg acgggacggt gcagccctct
     1381 cagcgcagcc gaagcacaac tggcgtttcg actggcgatg ggtcgcaccg atcggtcagg
     1441 tcctgctgac ggacagctgg gccagactcc gcggctgagg tagccaagaa tttcgttgag
     1501 ctagtctcag gtgtagaagc cgccgcaccc acaccccggg cccggccccc ggccgggtca
     1561 acacccaccc gctcatcagc aggatcagcg ccgccaggca cgtcagcccc atctcagaat
     1621 gatcggcgca gccgaggcat ccgccgacct cggagactgg atggccgacc ccggtcgagg
     1681 gggtcagatg tgcgtggtcc ccgccaaccg cgtcctcgtg actttcggcg tgcaccgcgg
     1741 acatcaggag cgtggcgtcg gtggaactcg ggctagccgg atggccggcg gcggtgtggt
     1801 tgctcgacag ctgatgcatg gcgagcaggc tcaacgccat agccacgacc ccgagcagca
     1861 gccagagccg ctgtcgggct ccgacggcca ccccaattcg caccatgtcc aaaccgtacc
     1921 cagcattgac cggaatatcg ccaccgcacg cgaccacgcc tggcaacagg cccggccacg
     1981 cgtgaccgcc gccccagtgg ttgctggaca atgcgcggtc ccagatgtcg atcctgatgg
     2041 cgtagcagca gggcacgatg cgggggtcac tccacgagtc ccacaggaac gtggtgctga
     2101 gcgcggcgac ctcgcgccac tcctcgacgt cgacctcctt gtcgtcgggt ggcccggcga
     2161 gcttcatgcg aacgaataga cagtgcttgc tacacagcca ccggcggatc aacaggtgtg
     2221 ctgttcgtgt tcgggcgaca cgcccgcggt aaacccgcga gcggcggatc ccaaggtgcg
     2281 gccacagtgt tcctgatgga ccccgaagcc ctgcagctgg cgagcatctt gacaggacat
     2341 cccttgccag accccggttg gacctttcgc ggcgggcttc agaacgagtt ggccgacagg
     2401 attcccccgg gccaccgctg ccgctgcccg caaaccaacc cttctaggta agcgataccc
     2461 cctaggggta tagtggtgaa cgtggacatg aacaagcacg agaacggaca cacctcgacg
     2521 ccgtcgcggg tgaaggaccc cgtctgcggc atggatgttg acccgacgac gagcgagcac
     2581 catctggtgc acgacggaac cacctactac ttctgctctg ggcaatgccg gaacaagttc
     2641 gacctcgccc ctgatgccta cctgtccccg gcaaccggtc acccgactgg acacggccac
     2701 ggcggcaacg ccgcgacgcc gccacccgga acggagtgga cctgtccgat gcatccggag
     2761 atcctgcgac ccggtccggg ttcgtgcccg atctgcggga tggctctcga gccggtcatg
     2821 gtgacagccg acagcgggcc aagcccggag ttggccgaca tgacccgccg tttctgggtc
     2881 ggggtagctt tgtccatccc tgtggtgatt ctggggatgg gcggagacct cgtcccagca
     2941 attcacgacg ccatctcccc gcgggcctcg gcctgggtcc aacttgttct ggcaaccccc
     3001 gtggtcttgt gggccggctg gccgttcttc gtgcgcgggt ggacctcggt ccgcacattg
     3061 aagctcaaca tgttcaccct gatcgcgatg ggcaccggtg tggcctggct gttcagcgtg
     3121 gtcgccaccc tggcgccaga cctcttccct gaggcgttcc ggatggacgg tgccgtcgat
     3181 gtctacttcg aagcagcatc cgtcatcacc accctcgtcc tcctcgggca ggtcctggaa
     3241 ctgcgagccc gtgaacagac atccggagcg atccgcgcct tgctcgacct caccccagac
     3301 acggcacacc ggctcaagtc ggacggaacc gaggaggagg tgactctcga tcaggtcacc
     3361 gtcggtgatc gcttccgggt tcgacccggc gagaaagttc ctgttgacgg ggacgtcgag
     3421 gaaggccgat cgacccttga cgagtcgttg gtgaccggtg agtcgatgcc agccaccaag
     3481 aaggtcggcg acagcgtcat cggcggcacc atcaaccaga ccgggtcgct gatcgtgcgt
     3541 gccgacaagg tcgggcggga caccatgctg gccaggatcg tgcagatggt tgccgaagcg
     3601 caacgctcgc gggcaccgat ccagcgggtg gcggatcggg tggctgcgtg gttcgtgcct
     3661 gccgtgatcg ccatcgcgat cgtcgcgttc ctggtgtggg caacggcggg tcccgacccg
     3721 cggctggccc acgcgctcgt ggtggccgta tccgtgctga tcatcgcgtg cccttgcgcc
     3781 ctgggtttgg ccacgccaat gtcgatcatg gttggggtgg ggcgaggcgc cgggctcgga
     3841 gtcctgatca agaacgcgga ggcgctcgaa cggatggaga aggtcgacac gctcgtcgtc
     3901 gataagaccg gcaccctgac cgagggcaag ccgtctgtga cacgcgtggt caccaccacc
     3961 gggttcgagg agcacgagat cctgcagttg gtggccgctg tcgagcaggc ctccgagcac
     4021 cctctcggtc tggcgatcgt gaacgcagcc cacgctgccg gcctgtccat cccgaacgtc
     4081 accgactgcg acgctccggt cggcaagggc gtcgtgggca ccgtcgagga ccagcgcatc
     4141 cgcgtcggca gcgccacgtt cctgaccggt gaagggatcg acccgagcgc actgacggca
     4201 caggcggacg agctgcgcgc cgacggtgcc accgtcattt tcgtcagctc cggtgaccaa
     4261 gtggccgcga tcatcgccat cgccgacccc
//
LOCUS       DAOSUG010000243         4284 bp    DNA     linear   ENV 10-JUN-2024
DEFINITION  MAG TPA_asm: Micropruina sp. isolate AMR_MDS_2195
            NODE_8757_length_4284_cov_4.068338, whole genome shotgun sequence.
ACCESSION   DAOSUG010000243 DAOSUG010000000
VERSION     DAOSUG010000243.1
DBLINK      BioProject: PRJNA925477
            BioSample: SAMN32864262
            Sequence Read Archive: SRR4236673
KEYWORDS    WGS; ENV; Metagenome Assembled Genome; MAG; Third Party Data; TPA;
            TPA:assembly.
SOURCE      Micropruina sp. (wastewater metagenome)
  ORGANISM  Micropruina sp.
            Bacteria; Bacillati; Actinomycetota; Actinomycetes;
            Propionibacteriales; Nocardioidaceae; Micropruina.
REFERENCE   1  (bases 1 to 4284)
  AUTHORS   Abdulkadir,N., Saraiva,J.P., Zhang,J., Stolte,S., Gillor,O.,
            Harms,H. and Rocha,U.
  TITLE     Genome-centric analyses of 165 metagenomes show that mobile genetic
            elements are crucial for the transmission of antimicrobial
            resistance genes to pathogens in activated sludge and wastewater
  JOURNAL   Microbiol Spectr 12 (3), e0291823 (2024)
   PUBMED   38289113
REFERENCE   2  (bases 1 to 4284)
  AUTHORS   Abdulkadir,N.
  TITLE     Direct Submission
  JOURNAL   Submitted (24-MAR-2023) Environmental Microbiology, Helmholtz
            Center for Environmental Research -UFZ, Permoserstr 15, Leipzig
            04318, Germany
COMMENT     The annotation was added by the NCBI Prokaryotic Genome Annotation
            Pipeline (PGAP). Information about PGAP can be found here:
            https://www.ncbi.nlm.nih.gov/genome/annotation_prok/
            
            ##Genome-Assembly-Data-START##
            Assembly Method        :: SPAdes v. 3.11.1
            Genome Representation  :: Full
            Expected Final Version :: Yes
            Genome Coverage        :: Not Applicable
            Sequencing Technology  :: Illumina MiSeq
            ##Genome-Assembly-Data-END##
            
            ##Genome-Annotation-Data-START##
            Annotation Provider               :: NCBI
            Annotation Date                   :: 05/04/2023 04:57:10
            Annotation Pipeline               :: NCBI Prokaryotic Genome
                                                 Annotation Pipeline (PGAP)
            Annotation Method                 :: Best-placed reference protein
                                                 set; GeneMarkS-2+
            Annotation Software revision      :: 6.5
            Features Annotated                :: Gene; CDS; rRNA; tRNA; ncRNA
            Genes (total)                     :: 2,776
            CDSs (total)                      :: 2,750
            Genes (coding)                    :: 2,714
            CDSs (with protein)               :: 2,714
            Genes (RNA)                       :: 26
            tRNAs                             :: 24
            ncRNAs                            :: 2
            Pseudo Genes (total)              :: 36
            CDSs (without protein)            :: 36
            Pseudo Genes (ambiguous residues) :: 0 of 36
            Pseudo Genes (frameshifted)       :: 2 of 36
            Pseudo Genes (incomplete)         :: 32 of 36
            Pseudo Genes (internal stop)      :: 2 of 36
            ##Genome-Annotation-Data-END##
FEATURES             Location/Qualifiers
     source          1..4284
                     /organism="Micropruina sp."
                     /mol_type="genomic DNA"
                     /submitter_seqid="NODE_8757_length_4284_cov_4.068338"
                     /isolate="AMR_MDS_2195"
                     /isolation_source="Activated sludge of municipal
                     wastewater treatment plants"
                     /db_xref="taxon:2737536"
                     /environmental_sample
                     /geo_loc_name="Canada"
                     /lat_lon="49.25 N 123.1 W"
                     /collection_date="2015-06-16"
                     /metagenome_source="wastewater metagenome"
                     /note="metagenomic"
     gene            <1..558
                     /locus_tag="PLF56_08660"
     CDS             <1..558
                     /locus_tag="PLF56_08660"
                     /inference="COORDINATES: protein motif:HMM:NF012798.2"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_function: GO:0003677 - DNA binding [Evidence IEA];
                     GO_process: GO:0006310 - DNA recombination [Evidence IEA];
                     GO_process: GO:0015074 - DNA integration [Evidence IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="tyrosine-type recombinase/integrase"
                     /protein_id="HOQ53670.1"
                     /translation="RRDHALLLTAVQTGLRLSELTGLTIQDVTAIMPGASVHTTGKGR
                     KERVTPLTRQTAAVLHEWLPQTGPSGPVFPTRRGTRLSADAVQRLVTKHAVTAALVCP
                     SIASKHVTPHTLRHTAAMTLLAAGVDTSVIALWLGHASATTTHIYLHADMTIKLRALA
                     RIPASGTKPGRYRPADDLIAFLEQL"
     gene            697..1623
                     /locus_tag="PLF56_08665"
                     /pseudo
     CDS             697..1623
                     /locus_tag="PLF56_08665"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_011726646.1"
                     /note="incomplete; partial in the middle of a contig;
                     missing N-terminus; Derived by automated computational
                     analysis using gene prediction method: Protein Homology.
                     GO_function: GO:0003677 - DNA binding [Evidence IEA];
                     GO_function: GO:0004803 - transposase activity [Evidence
                     IEA];
                     GO_process: GO:0006313 - transposition, DNA-mediated
                     [Evidence IEA]"
                     /pseudo
                     /codon_start=1
                     /transl_table=11
                     /product="transposase"
     gene            2112..2576
                     /locus_tag="PLF56_08670"
     CDS             2112..2576
                     /locus_tag="PLF56_08670"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_014845775.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="SRPBCC domain-containing protein"
                     /protein_id="HOQ53671.1"
                     /translation="MSANVIVVVEQEFAHPRPRVFRAWTAPEEMSQWRGSPGWHVERE
                     SVTSDLRTGGKHHHVKVRDDDPSVRVTTDAVFTEYFDPDVFVARQRITGDSGIDPNES
                     LELRVEFTAMGGDSTLVRIVQGPYDPAVAQYHAAGWEKELARLGTYLDGGQA"
     gene            2573..3970
                     /locus_tag="PLF56_08675"
     CDS             2573..3970
                     /locus_tag="PLF56_08675"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_013861091.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_function: GO:0022857 - transmembrane transporter
                     activity [Evidence IEA];
                     GO_process: GO:0055085 - transmembrane transport [Evidence
                     IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="MFS transporter"
                     /protein_id="HOQ53672.1"
                     /translation="MNTAGAPTTSAVVRTKPIMTMPQILLMNLGFFGIQYSFGMQQTA
                     INPIYTFLGASHDQLPLLNLAGPVTGLLIQPIIGALSDRTWVEKGFFRGRRRPFFLIG
                     AIGCAICLALFPFVWAVWMAVLLLWLLDASNNTAMEPYRAFIADRLPPAQLGKGFLAQ
                     SFFTGLGITLANLSLMFFQGVFGLGQSVGIPLWVYPAFLLGSVCSIATVLISVLSTPE
                     LEPTEKELAELRASKGGPGHFIAEIKDAIVDMPVEMRKLALVYLFQWYAMNIYWQYVS
                     LSVSQSLYGTTDARSEGYQLAVANTGVINGAYNIVTFCVAFGLVALARRLGAKWVHAI
                     ALWCATVGLLLFPQMPTLPLKVVAIVGLGIAWASIMGVPYIMIVRMVPSTRYGVYMGI
                     VNMMIVIPMLIQTLTFGAIYKNLLGDNPNNAIMAAGILLGIAGLVMLWVKEPPIVRDI
                     EPVGSGDETVTTKGA"
     gene            3981..>4284
                     /locus_tag="PLF56_08680"
     CDS             3981..>4284
                     /locus_tag="PLF56_08680"
                     /inference="COORDINATES: ab initio
                     prediction:GeneMarkS-2+"
                     /note="Derived by automated computational analysis using
                     gene prediction method: GeneMarkS-2+."
                     /codon_start=1
                     /transl_table=11
                     /product="universal stress protein"
                     /protein_id="HOQ53673.1"
                     /translation="MTPYGCIVVGTDGSALSDPTVARASFLAAAEEADLVIVCAYSGM
                     TMREGAKATATLGDTRTSVVLGREAASDALATATSTASGLGAQVKASLLVEGEAASA"
BASE COUNT          732 a         1507 c         1358 g          687 t
ORIGIN      
        1 cgccgtgacc acgctctgct ccttaccgcc gtgcagaccg gtcttcggct gtccgagttg
       61 accgggctca ccatccagga cgtcaccgcc atcatgcctg gcgcatccgt gcacaccacg
      121 ggcaagggcc ggaaggagcg cgtcaccccc ttgacccggc agactgcggc cgtcctgcat
      181 gaatggctgc cacaaaccgg gcccagcgga ccggttttcc cgacccgccg cggaacccgg
      241 ctatctgccg atgcagtcca acggctcgtc accaaacacg cggtgactgc ggcccttgtc
      301 tgcccatcga tcgcgtccaa gcatgtgaca ccgcacacgc tgcggcacac cgcagcgatg
      361 acgctgctcg cggccggtgt cgacacgtcg gtgatcgcct tgtggctcgg tcacgcaagc
      421 gcaacgacca cgcacatcta cctgcacgcg gacatgacca tcaagctgag agccctcgcc
      481 cgaatccccg cgtccggcac taagcccgga cgttaccgtc ccgcggacga cctcatcgcc
      541 ttcctcgaac aactgtgacg cccccaccgc gagggttatg ccgcgtcagc gacccccacc
      601 cggaccgctg accagcacaa acaccgctcc gcccacccgg acgtcggcat aagcgagacg
      661 tcggcataat tggagttatg ccgacgtcgg cataacagca aggaacgccg cctggagccg
      721 cagatcacga tcggcctgtt gaccgacgcg cgcgggttcc cgctgatggt tgaagcgttt
      781 gagggcaatc gcgccgagac caccacgatc atcccgtcga tccaggcctt ccaggccgcc
      841 catcagctcc ccgaggtgac cgtggtcgcc gacgcgggca tgctgtcgga tgggaacctg
      901 cgcgccctgt ccggggcggg gctgcggttc atcgtggggc agaagatccc cgacattccc
      961 tacgtcgtgc gccactggat ggacacccac cccggtgagc agccgcctga cgggctgatc
     1021 ctgacccagc cgtggtcgcg gggtccggcc ggccagcagg tgaaggagac gatctactac
     1081 cagtaccggg ctgatcgggc ccggcgcacg ctgcgcggca tcggtgagca ggtcggcaag
     1141 gccgaacgcg cggtcgcggg gaaggcgccg gtgaagcgga accggttcat cgccctcacc
     1201 ggcgcgcaga agtcggtgaa ccgggaactg gaggccaagg cccgcaccct ggcgggctgg
     1261 aagggctaca tcacgaactt ggccaacccg accccggagt acgtgatcgg ggcctaccac
     1321 cagctgtggc aggtcgagaa gtccttccgg atgtccaagt cggacctgaa ggcccggccg
     1381 gtctaccacc acctgaagga ctcgatcgac gcccacctca ccatcgtgtt cgccgccctc
     1441 gccgtcgccc gctggctcga gagcaccacc ggcgtcagca tcaaggccct cgtcaagacg
     1501 ctgcgtcgcc accgcaccat cgacatccaa gccggcgaca ccatcgtcac cgccgagaac
     1561 gccctacccg acgacgcaca agcctggctc gacgcgatcc accaagcgcg cgggcgacac
     1621 taaatgagcc aagtcgggtt gtgacgatcg tgaacggcca gtacgaccag atgcggctgg
     1681 acgccgtggc cgaggcgagc cagatgcacc tccaaacgtg attcgcccct gctggagggg
     1741 gcgcagattc actttcgagg agcgtttgcg gggcgcatcc cgacgccgcc agccaagccg
     1801 aacacggtgc cgggggagag gagcccgcgg ctgggggcgg gctcgaacgc gaagtccagc
     1861 ggctcacggc gcagatcgcg gcccagcccg acggcgtccc gaatgaggac gagggaggcg
     1921 accacgagga cgctgccgat gatgagcgac tctcgacgcc ctggtgagaa ttgtcgttcc
     1981 tgaccgttgc acagactccc gatggggtgc catgatgcgc aacgggatac cttggtgccg
     2041 gcagccaacg cgggttgcgc aaccgcctcg ggtggccccc gagcgccgag tcaaccccca
     2101 ctggaggtcg catgtcagca aatgtcatcg tcgtggtcga gcaggagttc gcccacccgc
     2161 ggccccgcgt gtttcgcgcc tggaccgcac ccgaggagat gtcccagtgg cggggcagcc
     2221 ctgggtggca cgtcgagcgg gagagcgtca cgtcggacct gcggaccggc ggcaagcacc
     2281 atcacgtgaa ggtgcgcgac gacgacccga gcgtccgcgt gaccacggac gccgtcttca
     2341 ccgagtactt cgacccggac gtgttcgtcg cgcgtcagcg catcacgggc gactccggca
     2401 tcgacccgaa cgagtccctc gaactgcgcg tcgagttcac cgccatgggc ggcgacagca
     2461 cgctcgtgcg catcgtccag gggccgtacg acccggccgt ggcgcagtat cacgccgccg
     2521 gctgggagaa ggaattggcc cgcttgggca cctacctgga tggaggacag gcatgaatac
     2581 cgcaggagcg ccgacgacgt cggcggtcgt ccgcacgaag cccatcatga ccatgccgca
     2641 gatcctcctg atgaacctcg ggttcttcgg catccagtac agcttcggca tgcagcagac
     2701 ggccatcaat ccgatctaca cgttcctggg cgcgtcacac gaccaattgc cgctgctcaa
     2761 cctggccggt ccggtcacgg gcctgctcat ccaacccatc atcggggccc tctcagaccg
     2821 aacatgggtg gagaagggct tcttccgcgg gcgccgacgc cccttcttcc tcatcggtgc
     2881 catcggctgc gcgatctgcc tggcgctgtt cccgttcgtg tgggcggtct ggatggcggt
     2941 tctcctgctg tggctcctgg acgcctccaa caacaccgcg atggagccct accgcgcgtt
     3001 catcgccgac cggctccccc cggcccaact cggcaagggc ttcctcgccc agtcgttctt
     3061 caccggcctg gggatcacgc tggccaactt gagcctgatg ttcttccagg gcgtcttcgg
     3121 cctgggtcag tccgtcggca tcccgctgtg ggtctacccg gcgttcctcc tcggctcggt
     3181 ctgctcgatc gccacggtgc tgatctcggt gctgtcaacg cccgagctcg agccgacgga
     3241 gaaagaactc gccgagctcc gcgccagcaa gggtggcccc ggccacttca tcgccgagat
     3301 caaggacgcc atcgtcgaca tgccggtcga gatgcggaag ctggcgttgg tgtacctctt
     3361 ccagtggtac gccatgaaca tctattggca atacgtgtcg ctgtcggtgt cccagtcgct
     3421 gtacggcacg acggacgccc gctccgaggg ctaccagctc gccgtcgcga acacaggcgt
     3481 catcaacggt gcctacaaca tcgtgacctt ctgtgtcgcg ttcgggctgg ttgccctggc
     3541 ccgccgcctg ggcgcgaagt gggtgcacgc catcgccctg tggtgcgcca cggtgggtct
     3601 gctcttgttc ccgcaaatgc cgaccctgcc gctcaaggtc gtcgcgatcg tgggcctggg
     3661 catcgcgtgg gcgtccatca tgggcgtgcc ttacatcatg atcgtgcgca tggttccctc
     3721 gacccgctac ggcgtctaca tgggcatcgt caacatgatg atcgtcatcc cgatgctcat
     3781 ccagacgctc acgttcggcg cgatctacaa gaacctgctg ggggacaacc ccaacaacgc
     3841 gatcatggcc gccggcatcc tgctgggcat cgccggcttg gtcatgctct gggtcaagga
     3901 gcccccgatc gtgcgcgaca tcgagccggt cggctctggc gacgagaccg tcaccacgaa
     3961 gggagcctga gatggcacgc atgactccgt acggatgcat cgtcgtcggc accgatggct
     4021 ctgccttgtc cgacccgacc gtcgcgaggg cctcgttctt ggccgctgcc gaggaggccg
     4081 acctcgtcat cgtctgcgcc tattcgggca tgacgatgcg cgaaggtgcg aaggcgacgg
     4141 ccaccctcgg tgacacccgg acctccgttg tgttgggacg cgaggcggcc agcgacgcgc
     4201 tggcgaccgc gacatccacg gcgtccggcc tgggcgcgca ggtcaaggct tcgctgctgg
     4261 tggagggcga ggctgcctcc gccc
//
LOCUS       DAOSUG010000244         4281 bp    DNA     linear   ENV 10-JUN-2024
DEFINITION  MAG TPA_asm: Micropruina sp. isolate AMR_MDS_2195
            NODE_8769_length_4281_cov_3.962849, whole genome shotgun sequence.
ACCESSION   DAOSUG010000244 DAOSUG010000000
VERSION     DAOSUG010000244.1
DBLINK      BioProject: PRJNA925477
            BioSample: SAMN32864262
            Sequence Read Archive: SRR4236673
KEYWORDS    WGS; ENV; Metagenome Assembled Genome; MAG; Third Party Data; TPA;
            TPA:assembly.
SOURCE      Micropruina sp. (wastewater metagenome)
  ORGANISM  Micropruina sp.
            Bacteria; Bacillati; Actinomycetota; Actinomycetes;
            Propionibacteriales; Nocardioidaceae; Micropruina.
REFERENCE   1  (bases 1 to 4281)
  AUTHORS   Abdulkadir,N., Saraiva,J.P., Zhang,J., Stolte,S., Gillor,O.,
            Harms,H. and Rocha,U.
  TITLE     Genome-centric analyses of 165 metagenomes show that mobile genetic
            elements are crucial for the transmission of antimicrobial
            resistance genes to pathogens in activated sludge and wastewater
  JOURNAL   Microbiol Spectr 12 (3), e0291823 (2024)
   PUBMED   38289113
REFERENCE   2  (bases 1 to 4281)
  AUTHORS   Abdulkadir,N.
  TITLE     Direct Submission
  JOURNAL   Submitted (24-MAR-2023) Environmental Microbiology, Helmholtz
            Center for Environmental Research -UFZ, Permoserstr 15, Leipzig
            04318, Germany
COMMENT     The annotation was added by the NCBI Prokaryotic Genome Annotation
            Pipeline (PGAP). Information about PGAP can be found here:
            https://www.ncbi.nlm.nih.gov/genome/annotation_prok/
            
            ##Genome-Assembly-Data-START##
            Assembly Method        :: SPAdes v. 3.11.1
            Genome Representation  :: Full
            Expected Final Version :: Yes
            Genome Coverage        :: Not Applicable
            Sequencing Technology  :: Illumina MiSeq
            ##Genome-Assembly-Data-END##
            
            ##Genome-Annotation-Data-START##
            Annotation Provider               :: NCBI
            Annotation Date                   :: 05/04/2023 04:57:10
            Annotation Pipeline               :: NCBI Prokaryotic Genome
                                                 Annotation Pipeline (PGAP)
            Annotation Method                 :: Best-placed reference protein
                                                 set; GeneMarkS-2+
            Annotation Software revision      :: 6.5
            Features Annotated                :: Gene; CDS; rRNA; tRNA; ncRNA
            Genes (total)                     :: 2,776
            CDSs (total)                      :: 2,750
            Genes (coding)                    :: 2,714
            CDSs (with protein)               :: 2,714
            Genes (RNA)                       :: 26
            tRNAs                             :: 24
            ncRNAs                            :: 2
            Pseudo Genes (total)              :: 36
            CDSs (without protein)            :: 36
            Pseudo Genes (ambiguous residues) :: 0 of 36
            Pseudo Genes (frameshifted)       :: 2 of 36
            Pseudo Genes (incomplete)         :: 32 of 36
            Pseudo Genes (internal stop)      :: 2 of 36
            ##Genome-Annotation-Data-END##
FEATURES             Location/Qualifiers
     source          1..4281
                     /organism="Micropruina sp."
                     /mol_type="genomic DNA"
                     /submitter_seqid="NODE_8769_length_4281_cov_3.962849"
                     /isolate="AMR_MDS_2195"
                     /isolation_source="Activated sludge of municipal
                     wastewater treatment plants"
                     /db_xref="taxon:2737536"
                     /environmental_sample
                     /geo_loc_name="Canada"
                     /lat_lon="49.25 N 123.1 W"
                     /collection_date="2015-06-16"
                     /metagenome_source="wastewater metagenome"
                     /note="metagenomic"
     gene            <1..366
                     /locus_tag="PLF56_08685"
     CDS             <1..366
                     /locus_tag="PLF56_08685"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_016669731.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="radical SAM/SPASM domain-containing protein"
                     /protein_id="HOQ53674.1"
                     /translation="ALTRQGMDRKQIEQHPISRSFGIRDGNGIVFVSHQGDVTPSGFL
                     PLVVGNVKQSRLVELYQSEPTMLALRDPDQFKGRCGACEFHDWCGGSRARAFAWTGDP
                     LESDPLCPYQPREQVVGAN"
     gene            638..1405
                     /locus_tag="PLF56_08690"
     CDS             638..1405
                     /locus_tag="PLF56_08690"
                     /EC_number="3.5.2.9"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_020385463.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_function: GO:0005524 - ATP binding [Evidence IEA];
                     GO_function: GO:0016810 - hydrolase activity, acting on
                     carbon-nitrogen (but not peptide) bonds [Evidence IEA];
                     GO_function: GO:0017168 - 5-oxoprolinase (ATP-hydrolyzing)
                     activity [Evidence IEA];
                     GO_process: GO:0005975 - carbohydrate metabolic process
                     [Evidence IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="5-oxoprolinase subunit PxpA"
                     /protein_id="HOQ53675.1"
                     /translation="MIRIDLNSDVGESFSRWTLGDDAALLAIVSSANIACGFHAGDPR
                     GIRSTLELAATNGVTVGAHVGYRDLAGFGRRDLDVAPDELTAETIYQIGALQALAASV
                     GTRVSYVKPHGALYNRIVHDERQAAAVVQAIRMLDPSLVLLGLPGAVVLDRAAEAGLA
                     TATEIFADRAYTAEGRLVPRRDPGAVLHDPHAIADRIVQAITTGRITAIDGSQVPING
                     ESICVHGDTPGAVAVARAVRSRLVEAGVTIGSFVPDS"
     gene            1406..3217
                     /locus_tag="PLF56_08695"
     CDS             1406..3217
                     /locus_tag="PLF56_08695"
                     /inference="COORDINATES: protein motif:HMM:TIGR00724.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="5-oxoprolinase/urea amidolyase family protein"
                     /protein_id="HOQ53676.1"
                     /translation="MVGHPDRRILPAGDRALLVELSGLRAALGFFDAVSEAGLWGVLE
                     AVPAARTVLVRFDPLLTEAAELTDRLATLTISPSRVARGREVTIAVRYDGDDLAEVAG
                     LLGVSERTVIEAHTSTPYRVAFGGFAPGFAYLSGGHPLLDVPRRQSPRTRIPAGAVAL
                     AGAFSAVYPRESPGGWQLIGHTDAALWNLDRRPPALLQPGDRVRFVEISSDEPSPVEH
                     VGTTSPVDHVGTAPLVKPVQTTTSEPPLVEPVETSPVELVQATPPVEPVQSTPPDAAS
                     PVESVQATPPDAASQVEPVETTQAVELARTTPPSEPAPPSVIILSPGAQALLQDEGRP
                     GLAGLGVPSSGALDRFALHQANRLVGNPSDAAVIEFAWGGAVSASGDLVVAVSGERAE
                     VEVVHLDHVARHDWPAPVLLHDGDTLRIVPRAGARSYLAVRGGFAVPPVLGSRSTDVL
                     SGLGPTPLATGARLLVGSPAGLPAVGDPEPWPERLDGAEVRLPVLLGPRDDWFTGEAI
                     GRLFGQSWTVSPHSNRVGLRLLGEHSLTRRTPDELPSEGLVAGAIQVPLSGQPVVFLA
                     DHPVTGGYPVIAVLRHSALDLAGQLAPGQRVRFVLDQ"
     gene            3382..3942
                     /locus_tag="PLF56_08700"
     CDS             3382..3942
                     /locus_tag="PLF56_08700"
                     /inference="COORDINATES: protein motif:HMM:NF025052.2"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="AAA family ATPase"
                     /protein_id="HOQ53677.1"
                     /translation="MATLILLNGPPRVGKSTLAARYADLHPGTLCLDIDSLHYLIGGW
                     RDIGAQVHDLLRPLALAMTHTHLAGGNDVIVPQYVADPDAVAAFELLAHRTGATFCEV
                     VLMSTRDEAIERFADRPDDTEWAAHNRRLVADLGGPEFLGAMHDRLADHLGRRPRSIV
                     IESRRDAIDDTFADLLRAVGDQPSPS"
     gene            complement(3976..>4281)
                     /locus_tag="PLF56_08705"
     CDS             complement(3976..>4281)
                     /locus_tag="PLF56_08705"
                     /inference="COORDINATES: ab initio
                     prediction:GeneMarkS-2+"
                     /note="Derived by automated computational analysis using
                     gene prediction method: GeneMarkS-2+."
                     /codon_start=1
                     /transl_table=11
                     /product="hypothetical protein"
                     /protein_id="HOQ53678.1"
                     /translation="GSLPVFGGALRLLSFLAVASIASGHERAATLGIRVGGSRGRCAK
                     SQLFGTRFAGPPRPHFANSKPSNAQACPSIGRGWYRRLTVASLVWIIGLPFLDSRSG"
BASE COUNT          585 a         1555 c         1465 g          676 t
ORIGIN      
        1 gccctgaccc ggcagggcat ggaccgcaag cagatcgagc aacacccgat ctcacgctcc
       61 ttcggcatcc gcgacggcaa cggcatcgtg ttcgtcagcc accagggcga cgtcactccg
      121 tccggctttc tgccgttggt ggtcggcaac gtcaagcagt cccgcctggt cgagttgtac
      181 cagagcgagc ccacgatgct cgccctgcgc gaccccgacc agttcaaggg acgctgcggc
      241 gcctgcgagt tccacgactg gtgcggcggc tcccgggccc gcgccttcgc ctggacgggc
      301 gacccgctcg agtccgatcc gctgtgcccc tatcagcctc gcgagcaggt ggtcggcgcc
      361 aactgaaccg cctcacgaga gggcaaccgg gcagccggtg aggcactgca cccacgttgc
      421 ctgcggtacc tgacgttgcc gacatgacgt tgccgccgtt gcagcgcggg caaagtcacc
      481 ttgcgcaggc aacgtcactg cgcgggtccc catgtgcggg catcgtcact ggacgggcgc
      541 cgacactgga cgggcgccga cactgggcga ccgacgccgc cgcgccgcta gtcgctgccc
      601 gcccgagcgt tctatggtcg aagcgaggag gtgcgccgtg atccggatcg acctgaacag
      661 cgatgtgggc gagagtttct cgcgctggac gctcggtgac gacgctgcgc tgctggccat
      721 cgtcagcagc gcgaacatcg cctgcggctt tcacgccggc gacccgcggg gcatccgttc
      781 caccctcgaa ctcgccgcga cgaacggggt caccgtgggg gcccatgtcg ggtaccgcga
      841 cctggcgggg ttcggccgtc gcgacctcga tgtggctccg gacgagttga ccgccgagac
      901 gatctatcag atcggcgcgc tgcaggcgtt ggccgccagc gtgggtaccc gagtcagcta
      961 cgtcaagccg cacggcgcgc tgtacaaccg catcgtccat gacgagcggc aagcggcggc
     1021 tgtggtccag gcgatcagga tgcttgatcc gtccctggtg ttgctgggcc tgccgggcgc
     1081 ggtggtgctc gatcgcgccg ccgaggcggg gctggcgacc gccaccgaga tcttcgccga
     1141 ccgcgcctac accgccgagg gccggctggt gccacgccgc gaccccggcg cggtgctgca
     1201 cgatccccac gcgatcgccg accgcatcgt gcaagcgatc acgaccggac ggatcaccgc
     1261 catcgacggt agtcaggtcc cgatcaacgg cgaatcgatc tgtgtgcacg gcgacactcc
     1321 gggcgcggtt gccgtggccc gtgccgttcg cagccggctg gtcgaggccg gggtgaccat
     1381 cggttccttc gtgccggact cctgaatggt cggccacccc gatcgccgca ttctgccggc
     1441 cggcgaccgg gccctgctcg tcgaactgtc cggcctgcgc gcggcgctgg gcttcttcga
     1501 cgccgtgagc gaggcaggcc tgtggggcgt tctcgaagcc gtgcccgcgg cccggactgt
     1561 gctcgtccgt ttcgaccccc tgctgaccga ggctgccgag ctgaccgacc ggctcgcgac
     1621 gctgacgatc tcccccagcc gcgtggccag ggggcgtgag gtgacgatcg ccgtccgcta
     1681 tgacggcgac gacctggccg aggtggccgg cctgctcggg gtgagcgagc gcaccgtgat
     1741 cgaggcccac acgtccacgc cctaccgggt ggcgttcggc ggcttcgccc cgggtttcgc
     1801 ctacctaagt ggcggccacc ctctgttgga cgtaccgcgc cggcagtcgc cgcgcacccg
     1861 cattccggct ggtgcagtgg cgttggcggg tgcgttctcc gcggtctacc cacgcgaaag
     1921 ccctggtggt tggcagttga tcggccacac cgacgccgcg ctgtggaacc tcgaccgccg
     1981 accgcccgcc cttcttcagc ccggcgaccg ggtcagattc gtcgagatct cgtcggacga
     2041 accttcgccg gttgagcatg tcgggaccac ctcgccggtc gaccatgtcg ggaccgcccc
     2101 gctggtcaag cctgtccaga ccaccacgtc cgaacccccg ctggtcgagc ccgtcgaaac
     2161 ctccccggtc gagcttgtcc aggccacccc gcccgtcgag cctgtccagt ccaccccacc
     2221 ggacgcggct tcgccagtcg agtctgtcca ggccacccca ccggacgcgg cttcgcaggt
     2281 cgagcctgtc gagaccaccc aggcggtcga gcttgcccga accaccccac cgtccgagcc
     2341 cgctcccccg tccgtcatca tcctgagccc cggcgcgcag gccctgctgc aggacgaggg
     2401 acgcccgggg ctcgccggct tgggcgtgcc ctcctcgggg gccctcgacc gattcgccct
     2461 gcatcaggcc aaccggctgg tcggcaaccc gtccgatgct gcggtgatcg agttcgcctg
     2521 gggcggcgcg gtgagcgcct ccggggacct ggtggtcgcg gtcagtggcg agcgcgccga
     2581 ggttgaggtc gtccatctcg accacgtggc gcggcacgac tggcccgctc cggtgctgct
     2641 gcacgacggc gacacgctgc gcatcgttcc gcgggccggt gcgcgcagct atcttgcggt
     2701 ccgcgggggc tttgcggtgc caccggtgct gggcagtcgg tcaacggacg tgttgtcggg
     2761 gctcggtccc actccgctgg cgacgggcgc gcgactgctc gtcggttcgc cggcggggct
     2821 gccggccgtc ggcgatcccg agccgtggcc cgagcggctg gacggtgctg aagtccggct
     2881 gcccgtgctg ctcggtccgc gcgacgactg gttcaccggc gaggcgatcg ggcggctctt
     2941 cggccagtcg tggacggtca gcccacactc gaaccgggtt gggctgcggc tgcttggcga
     3001 gcattccctc acccgcagga cccctgatga gttgcctagt gaaggcctgg tcgcgggagc
     3061 gatccaggtg cccttgagcg ggcagccggt ggttttcctg gccgatcatc cggtgaccgg
     3121 cggctacccg gtgatcgcgg tgctgcggca ctcggcgctc gacctggccg gccagttggc
     3181 gcccggtcag cgggtgcgct tcgtgctcga ccagtgacgg cgttgggcgc gcggcggtcg
     3241 gggtctcgac acgcttgacc agcgagggcg ccacgcccag ctcgtgaccg ttgattcagc
     3301 gtgtcgaaat ctgcggtctc gtcacgctgc cctggcgtcc gccgccgaac ggaggaccta
     3361 tacgcgtgct agcgtccgtc gatggccacc ctgatcctgc tgaacgggcc gcccagggtc
     3421 ggcaagtcca cgctggcggc ccgctacgcc gacctgcacc caggcaccct gtgcctcgac
     3481 atcgattcgc tgcactacct gatcggcggc tggcgtgaca tcggcgccca ggtgcatgac
     3541 ctgctcaggc cgctggccct cgccatgacg cacactcatc tggccggcgg aaacgacgtc
     3601 atcgtgccgc agtacgtggc cgacccggac gccgttgccg ccttcgaact gctcgcccac
     3661 cgcaccggcg cgaccttctg cgaggtcgtc ctgatgtcta cgcgtgacga ggcgatcgag
     3721 cggttcgccg accgtccgga cgacaccgag tgggcggccc acaaccgccg cctggtggcc
     3781 gacctgggcg gcccggagtt tctgggggcc atgcatgacc ggctcgccga ccacctggga
     3841 cggcgtccgc gctcgatcgt gatcgagagc cggcgcgacg ccattgacga caccttcgcc
     3901 gacctgctgc gcgccgtcgg cgaccagcca tcgccatcgt gactgcgccg acttgacccg
     3961 tgccggcaca gctccctatc ctgaacggct gtccagaaaa ggaagtccga tgatccacac
     4021 cagcgaagcc accgtgagcc ggcgatacca gccgcgcccg atgctggggc atgcctgcgc
     4081 gttcgacggc ttcgagtttg cgaagtgggg tcgaggcggg cccgcgaacc tcgtgccgaa
     4141 gagctgactc ttcgcgcagc gaccacgcga gccgcccact cgaatgccga gtgtggcggc
     4201 tctttcatgc ccgctggcga tcgacgccac ggccaggaag ctcaggaggc gaagcgcccc
     4261 gccgaagacg gggaggctgc c
//
LOCUS       DAOSUG010000245         4252 bp    DNA     linear   ENV 10-JUN-2024
DEFINITION  MAG TPA_asm: Micropruina sp. isolate AMR_MDS_2195
            NODE_8822_length_4252_cov_3.944722, whole genome shotgun sequence.
ACCESSION   DAOSUG010000245 DAOSUG010000000
VERSION     DAOSUG010000245.1
DBLINK      BioProject: PRJNA925477
            BioSample: SAMN32864262
            Sequence Read Archive: SRR4236673
KEYWORDS    WGS; ENV; Metagenome Assembled Genome; MAG; Third Party Data; TPA;
            TPA:assembly.
SOURCE      Micropruina sp. (wastewater metagenome)
  ORGANISM  Micropruina sp.
            Bacteria; Bacillati; Actinomycetota; Actinomycetes;
            Propionibacteriales; Nocardioidaceae; Micropruina.
REFERENCE   1  (bases 1 to 4252)
  AUTHORS   Abdulkadir,N., Saraiva,J.P., Zhang,J., Stolte,S., Gillor,O.,
            Harms,H. and Rocha,U.
  TITLE     Genome-centric analyses of 165 metagenomes show that mobile genetic
            elements are crucial for the transmission of antimicrobial
            resistance genes to pathogens in activated sludge and wastewater
  JOURNAL   Microbiol Spectr 12 (3), e0291823 (2024)
   PUBMED   38289113
REFERENCE   2  (bases 1 to 4252)
  AUTHORS   Abdulkadir,N.
  TITLE     Direct Submission
  JOURNAL   Submitted (24-MAR-2023) Environmental Microbiology, Helmholtz
            Center for Environmental Research -UFZ, Permoserstr 15, Leipzig
            04318, Germany
COMMENT     The annotation was added by the NCBI Prokaryotic Genome Annotation
            Pipeline (PGAP). Information about PGAP can be found here:
            https://www.ncbi.nlm.nih.gov/genome/annotation_prok/
            
            ##Genome-Assembly-Data-START##
            Assembly Method        :: SPAdes v. 3.11.1
            Genome Representation  :: Full
            Expected Final Version :: Yes
            Genome Coverage        :: Not Applicable
            Sequencing Technology  :: Illumina MiSeq
            ##Genome-Assembly-Data-END##
            
            ##Genome-Annotation-Data-START##
            Annotation Provider               :: NCBI
            Annotation Date                   :: 05/04/2023 04:57:10
            Annotation Pipeline               :: NCBI Prokaryotic Genome
                                                 Annotation Pipeline (PGAP)
            Annotation Method                 :: Best-placed reference protein
                                                 set; GeneMarkS-2+
            Annotation Software revision      :: 6.5
            Features Annotated                :: Gene; CDS; rRNA; tRNA; ncRNA
            Genes (total)                     :: 2,776
            CDSs (total)                      :: 2,750
            Genes (coding)                    :: 2,714
            CDSs (with protein)               :: 2,714
            Genes (RNA)                       :: 26
            tRNAs                             :: 24
            ncRNAs                            :: 2
            Pseudo Genes (total)              :: 36
            CDSs (without protein)            :: 36
            Pseudo Genes (ambiguous residues) :: 0 of 36
            Pseudo Genes (frameshifted)       :: 2 of 36
            Pseudo Genes (incomplete)         :: 32 of 36
            Pseudo Genes (internal stop)      :: 2 of 36
            ##Genome-Annotation-Data-END##
FEATURES             Location/Qualifiers
     source          1..4252
                     /organism="Micropruina sp."
                     /mol_type="genomic DNA"
                     /submitter_seqid="NODE_8822_length_4252_cov_3.944722"
                     /isolate="AMR_MDS_2195"
                     /isolation_source="Activated sludge of municipal
                     wastewater treatment plants"
                     /db_xref="taxon:2737536"
                     /environmental_sample
                     /geo_loc_name="Canada"
                     /lat_lon="49.25 N 123.1 W"
                     /collection_date="2015-06-16"
                     /metagenome_source="wastewater metagenome"
                     /note="metagenomic"
     gene            <1..1370
                     /locus_tag="PLF56_08710"
     CDS             <1..1370
                     /locus_tag="PLF56_08710"
                     /inference="COORDINATES: protein motif:HMM:NF024910.2"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_component: GO:0016020 - membrane [Evidence IEA];
                     GO_function: GO:0022857 - transmembrane transporter
                     activity [Evidence IEA];
                     GO_process: GO:0055085 - transmembrane transport [Evidence
                     IEA]"
                     /codon_start=3
                     /transl_table=11
                     /product="amino acid permease"
                     /protein_id="HOQ53679.1"
                     /translation="LWQLTAIGVGGIIGVGIFTLAGVVAHGDANNPAAGPAVVISFLI
                     AGLASAAAALAYAEFAGMIPKAGSAYTYGYVGLGEIAGWFIGWDLLLEYTVIVAVVAI
                     GISGYTTAFLNGVGLELPAGLAHAWSPDTPDGAVNVPAIAVCLIVTWILSRGAKSFGR
                     FELVAVGLKILVIVAIIGVGIFHVQPSNFTPFMPNGFGPVLAGSATVFFAVFGYDAMS
                     TAAEEAVDGKKHMPKAILLSLAIAMVLYVMATLVLTGMQSYADLNPTSAFASAFDSVG
                     LPAFATVISAFAVVSILTVMLTFLLGVTRVWFSMSRDGLLPGWFAKVDTKRGVPQRVT
                     WIAGLASTVLAGVLPIRLVADLANIGILAAFVVVCAAVIVMRRTRPDAHRTFRIPFMP
                     VVPLVGLVASAMLMLQLHWQTWLRFAIWLIIGMIIYWFYGRKHSLLNPDSPRHREAAA
                     TPAAD"
     gene            1440..3058
                     /gene="aldA"
                     /locus_tag="PLF56_08715"
                     /pseudo
     CDS             1440..3058
                     /gene="aldA"
                     /locus_tag="PLF56_08715"
                     /inference="COORDINATES: protein motif:HMM:NF012398.2"
                     /note="frameshifted; Derived by automated computational
                     analysis using gene prediction method: Protein Homology.
                     GO_function: GO:0016620 - oxidoreductase activity, acting
                     on the aldehyde or oxo group of donors, NAD or NADP as
                     acceptor [Evidence IEA]"
                     /pseudo
                     /codon_start=1
                     /transl_table=11
                     /product="aldehyde dehydrogenase"
     gene            complement(3156..3545)
                     /locus_tag="PLF56_08720"
     CDS             complement(3156..3545)
                     /locus_tag="PLF56_08720"
                     /inference="COORDINATES: ab initio
                     prediction:GeneMarkS-2+"
                     /note="Derived by automated computational analysis using
                     gene prediction method: GeneMarkS-2+."
                     /codon_start=1
                     /transl_table=11
                     /product="GNAT family N-acetyltransferase"
                     /protein_id="HOQ53680.1"
                     /translation="MRRALRSERERDARTYVTVDTSTGRVAGYYSLSAFSVVRSSAPG
                     ALSRNAPDPVPAVLLGRLAVDQQFHGARLGTSVLHDAVHNAITAAERIGLRALIVDAL
                     DEGAAAFYVRHGFKPFPGQALRLFHRL"
     gene            complement(3629..3892)
                     /locus_tag="PLF56_08725"
     CDS             complement(3629..3892)
                     /locus_tag="PLF56_08725"
                     /inference="COORDINATES: protein motif:HMM:NF020265.2"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="DUF1778 domain-containing protein"
                     /protein_id="HOQ53681.1"
                     /translation="MAEKSARLDLRLTEPQKQLIEQAAELSGSTLAGFAISELVDAAS
                     NVVARSRSMVLDAQEWEMFVAALDSPDDQAWTELKAMAPVWER"
BASE COUNT          693 a         1456 c         1387 g          716 t
ORIGIN      
        1 ggctgtggca gctcaccgcc atcggcgtcg gcggcatcat cggcgtcggc atcttcaccc
       61 tggccggcgt ggtcgcgcac ggggatgcca acaaccccgc ggccggcccg gccgtggtga
      121 tctcgttcct gatcgcaggc ctcgcctcgg ccgctgcggc actcgcctac gccgagttcg
      181 cgggcatgat tcccaaggcc ggctcggcct acacctacgg ctatgtcggg ctcggcgaga
      241 tcgccggctg gttcatcggc tgggatctac tgctcgagta caccgtgatc gtcgccgtgg
      301 tggcgatcgg catctcgggc tacaccacgg cctttctgaa cggggtcggc ctcgagttgc
      361 ccgccggcct ggcccatgcc tggtcgcccg acaccccgga cggtgcggtc aacgtgcccg
      421 ccattgcggt ctgcctgatc gtgacctgga ttctcagccg cggcgccaag agcttcggac
      481 gcttcgaact ggtcgccgtc ggactgaaga tcctggtcat cgtcgcgatc atcggggtcg
      541 gcatctttca cgtgcagccg agcaacttca cgccgttcat gccgaacggt ttcgggccgg
      601 tactcgccgg gtcggccacg gtgttcttcg ccgtattcgg ctacgacgcg atgagcaccg
      661 ccgccgaaga ggccgtggac ggcaagaagc acatgccgaa ggcgattctg ttgtcgctgg
      721 ccatcgccat ggtgctgtac gtgatggcca ccctggtgct gaccggcatg cagtcctacg
      781 ccgacctcaa ccccaccagc gccttcgcgt ctgctttcga ttcggtcggg ctgccggcgt
      841 tcgcgaccgt catttcggcc ttcgccgtgg tgtcgatcct caccgtgatg ctgaccttcc
      901 tgctcggtgt cacccgggtc tggttctcga tgagccggga cgggctgctg cccggctggt
      961 tcgccaaggt ggacaccaag cgcggggtgc cgcagcgggt cacctggatc gcgggcctcg
     1021 cctcgaccgt gctcgccggg gtgttgccga tccgcctggt ggccgacctg gccaacatcg
     1081 gcatcctggc tgccttcgtg gtggtctgtg cggccgtgat cgtgatgcgc cgtacccgtc
     1141 cggacgcgca tcgcacgttc cgcattccgt tcatgcccgt cgtacccctg gtcggcctgg
     1201 tcgcctcggc gatgttgatg ctgcagctgc actggcagac ctggttgcgc ttcgcgatct
     1261 ggttgatcat cggcatgatc atctactggt tctacggccg caagcactcc ctgctcaacc
     1321 ccgacagccc ccggcaccgc gaggcggcgg cgacccctgc cgccgactga gcgtcccccg
     1381 cacgcctgcc gcggtggggg cgcttcggct gccttcgctc cgttggggcg cgctcaacaa
     1441 tgtggggccc gcgtaggtgc gcgagcccca cctggtccgg cgagcccgat cgcacgcctt
     1501 cggccgctgc cgacggtggg ctaatcgggg ctaatcgggt tgtcaggttt cgcctccggc
     1561 ggcctggcgc ttgtgctacg acggtggcag tacaacgacg tactgactca tcagagaggc
     1621 atcaccacgt gcagacgtat cagcagttca tcaacggctc gctcgtcgac tcccactcaa
     1681 ccgattggct cgaggtcgag aacccctaca ccaacgagat catcgcccgt gcacccaagg
     1741 gcgacgccga ggacgccaat cgcgcggtcg aggcggccaa ggccgcgcag ccggcgtggg
     1801 cgaagtgggc gccggtggct cgcgcggcct acctcaagca gttcgccgag ttgatccggg
     1861 cgaaccgggt ggaactcgcc accaccctgg ccaaggaaca ggccaagatc atgccgctgg
     1921 cccaggtcga gatcgacgtc accgccgagt acttcgacta ctacgccggc tgggcccgca
     1981 tctacgaggg tgagatcatc aactccgacg atccccgcga gaacatcctg ctgttccaga
     2041 aaccgatcgg cgtggtggcc ggcatctgcc cgtggaactt ccccttcttc gtgatggccc
     2101 gcaaggtggc ccccgcgttg ctgaccggct gctcgatcgt gctgaagccc tccagcaaca
     2161 ccccggccac gaccttcgag ttcgcgaagc tggtcgccgg gctcgacctg cccgcgggcg
     2221 tgctgaactt cgtctccggc ggtggcgcca ccctcggcga cgcgctggtg aagcatcccg
     2281 acgtcgacat ggtctcgctc accggttcgg tggaggcagg cgtccagatc atgcacaacg
     2341 cggccgacaa gatcatgaag acctcactcg aactgggcgg caaggccccc gcgatcgtct
     2401 ttccggacgc cgacatcgac ctcgcggtca agggcgtcac cgactcccgg ctgatctact
     2461 cgggacaggt gtgcaactgc tgcgagcggg tctacgtgca cgccgacgtc tacgacgagt
     2521 tcgtcgccaa gctgaaggtc gctttcgagg agtccgccgt gggtgatccc ttcgcggacc
     2581 cgacaccggc ctacagctcc cagatcgaca aggcgcaact ggagaagatc gacggcatgg
     2641 tgcagcgcgc ggtggccgcg ggcgccgagg tgctcaccgg cggcaagccg acctgggcac
     2701 cggctacttc tactccccca ccctgatcac cggggcggcg caggactccg agatcgtgca
     2761 gaaggaggtc ttcgggccgg tgctgccgat cgtgaattgg gacgactacg accaggtcat
     2821 cgccgacgcc aacgacatcg agtacgggct gacgtcctcg atcttcacca ccaacgtgaa
     2881 cacggtgatg caggccatca aggacctcaa cttcggcgag acctacgtca accgcgagca
     2941 cttcgagggc atccagggct tccacgccgg ctggcgcaag tccgggatcg gcggcgcgga
     3001 cggcaagcac ggcctgcagg agtacttgca gtcgcaggtg gtgtacgtcc gctactgagg
     3061 cactgcagac aacagtgagg cgggcccgtg gccgcgcgtc ccgatgatct cggctcgctc
     3121 gaccacggtc tcgccgcctc cacctggacg gatcgctaca ggcggtggaa gagccgcagg
     3181 gcctgaccgg ggaacggctt gaacccatgg cggacgtaga acgctgccgc accctcgtcg
     3241 agggcgtcga caatgagggc gcgcagcccg atgcgctcgg ccgccgtgat ggcgttgtgg
     3301 acggcgtcat gcagcacaga ggtgcccagc cgggcgccgt ggaactgctg gtcgaccgcg
     3361 agccggccca gcagcaccgc cgggacaggg tcgggcgcgt ttcgggacag cgcgcccggc
     3421 gcgctcgacc tcaccaccga gaacgccgag aggctgtagt agccggcgac ccgcccggtg
     3481 gacgtgtcga cggtgacgta ggtgcgggcg tcgcgttccc gctcggaacg caacgcccgc
     3541 cgcaccagcc acccgttcag ttctgcgcgg ccacagtcga agccggcaat gtcatcgtct
     3601 gggcgcagcg gtcgcggtgg ggttaagctc accgttccca caccggggcc atggccttca
     3661 actcggtcca cgcctggtcg tcaggcgagt ccagggccgc gacgaacatc tcccattcct
     3721 gggcgtcgag caccatcgat cgggagcggg cgacaacgtt cgaggccgcg tccacaagtt
     3781 cggagatcgc gaacccggcc agcgtgctgc cactgagctc ggcagcctgc tcgatcagtt
     3841 gcttctgcgg ttcggtgagg cgcaagtcga gtcgcgccga cttctcagcc atggccccaa
     3901 ttgtacggta gaacaccgca cgggcaagat gtgcgatcgc gatagtgcca cctacccgcc
     3961 agtatcccac gaacgacggg gcacaacccc ggcgacagac acgacggctt ggccaggcaa
     4021 ctcttctgag gggggtcccc agggaccagg gtgcgctcgc ggacagccgc gcaaagacca
     4081 ggacaatgcc gtgctggcgg actcaggaca gcgcccaatc gatgtccgaa acccgctaat
     4141 gggccgacct gagaggaacc ttacgtgcac gtggagtgca cgagaggctg gctacggttc
     4201 cgtaaccagc ctctgtcaag gtaatagacc cgtcgggctg acaggatttg aa
//
LOCUS       DAOSUG010000246         4249 bp    DNA     linear   ENV 10-JUN-2024
DEFINITION  MAG TPA_asm: Micropruina sp. isolate AMR_MDS_2195
            NODE_8825_length_4249_cov_4.444921, whole genome shotgun sequence.
ACCESSION   DAOSUG010000246 DAOSUG010000000
VERSION     DAOSUG010000246.1
DBLINK      BioProject: PRJNA925477
            BioSample: SAMN32864262
            Sequence Read Archive: SRR4236673
KEYWORDS    WGS; ENV; Metagenome Assembled Genome; MAG; Third Party Data; TPA;
            TPA:assembly.
SOURCE      Micropruina sp. (wastewater metagenome)
  ORGANISM  Micropruina sp.
            Bacteria; Bacillati; Actinomycetota; Actinomycetes;
            Propionibacteriales; Nocardioidaceae; Micropruina.
REFERENCE   1  (bases 1 to 4249)
  AUTHORS   Abdulkadir,N., Saraiva,J.P., Zhang,J., Stolte,S., Gillor,O.,
            Harms,H. and Rocha,U.
  TITLE     Genome-centric analyses of 165 metagenomes show that mobile genetic
            elements are crucial for the transmission of antimicrobial
            resistance genes to pathogens in activated sludge and wastewater
  JOURNAL   Microbiol Spectr 12 (3), e0291823 (2024)
   PUBMED   38289113
REFERENCE   2  (bases 1 to 4249)
  AUTHORS   Abdulkadir,N.
  TITLE     Direct Submission
  JOURNAL   Submitted (24-MAR-2023) Environmental Microbiology, Helmholtz
            Center for Environmental Research -UFZ, Permoserstr 15, Leipzig
            04318, Germany
COMMENT     The annotation was added by the NCBI Prokaryotic Genome Annotation
            Pipeline (PGAP). Information about PGAP can be found here:
            https://www.ncbi.nlm.nih.gov/genome/annotation_prok/
            
            ##Genome-Assembly-Data-START##
            Assembly Method        :: SPAdes v. 3.11.1
            Genome Representation  :: Full
            Expected Final Version :: Yes
            Genome Coverage        :: Not Applicable
            Sequencing Technology  :: Illumina MiSeq
            ##Genome-Assembly-Data-END##
            
            ##Genome-Annotation-Data-START##
            Annotation Provider               :: NCBI
            Annotation Date                   :: 05/04/2023 04:57:10
            Annotation Pipeline               :: NCBI Prokaryotic Genome
                                                 Annotation Pipeline (PGAP)
            Annotation Method                 :: Best-placed reference protein
                                                 set; GeneMarkS-2+
            Annotation Software revision      :: 6.5
            Features Annotated                :: Gene; CDS; rRNA; tRNA; ncRNA
            Genes (total)                     :: 2,776
            CDSs (total)                      :: 2,750
            Genes (coding)                    :: 2,714
            CDSs (with protein)               :: 2,714
            Genes (RNA)                       :: 26
            tRNAs                             :: 24
            ncRNAs                            :: 2
            Pseudo Genes (total)              :: 36
            CDSs (without protein)            :: 36
            Pseudo Genes (ambiguous residues) :: 0 of 36
            Pseudo Genes (frameshifted)       :: 2 of 36
            Pseudo Genes (incomplete)         :: 32 of 36
            Pseudo Genes (internal stop)      :: 2 of 36
            ##Genome-Annotation-Data-END##
FEATURES             Location/Qualifiers
     source          1..4249
                     /organism="Micropruina sp."
                     /mol_type="genomic DNA"
                     /submitter_seqid="NODE_8825_length_4249_cov_4.444921"
                     /isolate="AMR_MDS_2195"
                     /isolation_source="Activated sludge of municipal
                     wastewater treatment plants"
                     /db_xref="taxon:2737536"
                     /environmental_sample
                     /geo_loc_name="Canada"
                     /lat_lon="49.25 N 123.1 W"
                     /collection_date="2015-06-16"
                     /metagenome_source="wastewater metagenome"
                     /note="metagenomic"
     gene            complement(<1..898)
                     /locus_tag="PLF56_08730"
     CDS             complement(<1..898)
                     /locus_tag="PLF56_08730"
                     /inference="COORDINATES: protein motif:HMM:NF013700.2"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="peptidoglycan DD-metalloendopeptidase family
                     protein"
                     /protein_id="HOQ53682.1"
                     /translation="MATPPRHERPEPASTGFRLWRERIRASRPRRGASVDPVPLDRPA
                     QTAGALHDGGRRWADPEPEAAPRRVNAGPAPGHGRGKRAARAPIARSSWVLNGAAIAI
                     AVMLAPVLTASSATTAALNASSSVAGTPEPSISWVPTDPRFSPAPGQQPLYDRSAQRD
                     AVDADDTDDTLAAPPPRASVTPNGSQFVSPVPGAITGGFGMRFHPILHYWRMHNGVDM
                     HAACGTPVVAAYRGTVVQAGRNGGYGNLVVIDLGVYQGKPTLTKYAHLSRIGVTVGQK
                     VETGQGIALSGTTGLSTGCHLHF"
     gene            complement(996..2477)
                     /locus_tag="PLF56_08735"
     CDS             complement(996..2477)
                     /locus_tag="PLF56_08735"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_013863077.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_function: GO:0004357 - glutamate-cysteine ligase
                     activity [Evidence IEA];
                     GO_process: GO:0042398 - cellular modified amino acid
                     biosynthetic process [Evidence IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="glutamate-cysteine ligase family protein"
                     /protein_id="HOQ53683.1"
                     /translation="MGRDIAARTYTREQRQRYREKVQRCLDVFEQMLSAHSFEFDEPL
                     VGMEIELNLVDADQRPAMDNADVLAAIADPEYQTELGRFNIEFNVPPGRLSGAALADQ
                     EQALRASMNAAEQRSSEVGTHIVMTGILPTVTMETFQGAWMSDNVRYAAINDAVLAAR
                     GEDISIDIEGTTGETLTCYIDSVAPEAACTSMQLHLQVRPEEFANHWNAAQVLAGPQL
                     ALAANSPFLMGSRLWAETRIVLFAQATDTRPIEYANQGVRPRVWFGERWITSIFDLFE
                     ENARWFPALLPELDDEDPQQVLDAGGAPQLSEMRLHNGTIYRWNRPIYDVAGGQPHLR
                     LENRVLPAGPSVVDTLANAAFYYGTLRMLAFEDRPVWSRMAFSTAHANFLEGARQGVD
                     ADLFWPGVGELPASELILRHLLPLAHEGLDAWGVDPAVRDRLLGVVEGRCKTGMNGAE
                     WQANAVQRFEAAGMSRPEALRRMVAAYAECMHANRPVHTWELP"
     gene            2639..3433
                     /locus_tag="PLF56_08740"
     CDS             2639..3433
                     /locus_tag="PLF56_08740"
                     /inference="COORDINATES: protein motif:HMM:NF013757.2"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_function: GO:0003677 - DNA binding [Evidence IEA];
                     GO_function: GO:0003700 - DNA-binding transcription factor
                     activity [Evidence IEA];
                     GO_process: GO:0006355 - regulation of DNA-templated
                     transcription [Evidence IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="IclR family transcriptional regulator"
                     /protein_id="HOQ53684.1"
                     /translation="MSPVRGGTDVTPPGAAPAVSRGIAVLDALAGSENGSMTLSDLSR
                     ALGVPKSSTSAICTALELGGMVRRDDLGYSLGRKLVELGGAYLARLDQVSEFHRACLE
                     STVFRAETLRLSTAAGIDCLCLARYEGHPAIRLTTNIGDRLPASSCAQGKALLARLDD
                     SEIERLYHGIDELPRLTSRSRRSVPRLLKDIRLARARGYAIDEEESADSVMGLAVVVP
                     TRGVRAPILAISVTKHKSDWNEPTRERLVDELKRVATMLGNPMATG"
     gene            3783..>4249
                     /locus_tag="PLF56_08745"
     CDS             3783..>4249
                     /locus_tag="PLF56_08745"
                     /inference="COORDINATES: protein motif:HMM:NF012312.2"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_component: GO:0016020 - membrane [Evidence IEA];
                     GO_function: GO:0022857 - transmembrane transporter
                     activity [Evidence IEA];
                     GO_process: GO:0055085 - transmembrane transport [Evidence
                     IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="MFS transporter"
                     /protein_id="HOQ53685.1"
                     /translation="MSESHAIHGTSNEHEVVDPVQLRRATLASSVGSALEYYDFYIYG
                     LASALIFGELFFKPLGAAGALIAAFGTYAVGFAARPVGGLVFGHLGDKLGRKLILIIT
                     IALMGGSSMLIGLLPTYDQVGMWGAILLILLRIAQGLGAGAEQAGATVLISEFA"
BASE COUNT          665 a         1452 c         1416 g          716 t
ORIGIN      
        1 cgaagtgcag atggcacccg gtcgacagcc cggtggtgcc cgacagtgcg atgccctggc
       61 cggtctcgac cttctgtccg acggtcaccc cgatgcgcga caggtgcgcg tacttggtga
      121 gtgtcggctt gccctggtag acgcccaggt cgatcaccac caggttgccg tagccgccgt
      181 tgcggccggc ctgcacgact gtgccccggt aggccgcaac caccggcgtg ccgcacgccg
      241 cgtgcatgtc aacgccgttg tgcatgcgcc agtagtgcag gatcgggtgg aaccgcatgc
      301 cgaaaccgcc cgtgatcgca cccggcacgg gcgacacgaa ctggctgccg ttcggggtga
      361 ccgaggcgcg gggcggcggt gccgccaacg tgtcgtcggt gtcatcggca tcgacggcgt
      421 cacgctgcgc cgagcggtcg tacagcggct gctgaccggg tgcgggcgag aaccgcgggt
      481 cggtgggcac ccagctgatg ctcggctcgg gggtgccggc caccgacgat gaggcattga
      541 gcgccgcggt ggtggccgac gaggccgtca gcaccggagc cagcatcacc gcgatagcga
      601 tcgcggcacc gttgagcacc cacgaactgc gcgcaatggg ggcgcgggcc gcccgcttgc
      661 cgcggccgtg tcctggtgcc gggccggcgt tcactcgtcg cggcgcagcc tcgggctcag
      721 gatcggccca ccgccgtcca ccatcgtgca gggcaccggc ggtctgtgcg ggccggtcga
      781 ggggtacggg gtcaacgctc gcaccgcggc gcggccggga cgcacggatg cgctcgcgcc
      841 agagccggaa cccggtggac gccggttctg gccgctcgtg ccgcggtggt gtcgccatcg
      901 gcatccttac ctcacgtgtg aactcgcgaa tggtagcaag catgagcccg gataccaccg
      961 gtgcgatacc atttctgagg ctttctcatt cagtattagg gcagctccca ggtatggacc
     1021 ggcctattcg cgtgcatgca ttccgcgtag gcggccacca ttcgccgcag cgcctcgggc
     1081 cgagacattc cagcggcctc gaaacgctgc acggcattgg cctgccactc ggcaccgttc
     1141 atgccggtct tgcaccggcc ctccaccaca ccgagcagcc ggtcacgcac ggcggggtcg
     1201 acaccccacg cgtccaggcc ctcgtgggcc agcggcagca ggtggcgcag gatcagctcg
     1261 ctggcgggca gctcacccac tccgggccag aacaggtcgg catcgacgcc ctgccgggca
     1321 ccctccagaa agttcgcgtg ggcggtcgag aacgccatcc ggctccagac cggacgatcc
     1381 tcgaaggcga gcatgcgcag cgtcccgtag tagaaagcgg cgttggcaag ggtgtccacg
     1441 acgctcggtc cggcaggcag cacgcggttc tcgaggcgca ggtgcggctg cccgccggcg
     1501 acgtcgtaga tcggacggtt ccagcggtag atagtgccgt tgtgcagccg catctcgctg
     1561 agttgcggcg cgcccccggc gtccagcacc tgttgcggat cctcgtcgtc cagttcgggc
     1621 aacagggccg ggaaccagcg ggcgttctct tcgaacaggt cgaagatcga ggtgatccac
     1681 cgctcgccga accacacccg cggccgcacg ccctggttgg cgtactcgat cggacgggtg
     1741 tcggtcgctt gcgcgaacaa cacgatgcgg gtttcggccc acaagcgact acccatcagg
     1801 aaaggtgaat tcgcggccag ggccaattgc ggcccggcca gcacctgcgc cgcattccaa
     1861 tggttggcga attcttctgg gcgcacctgc agatgcagtt gcatcgacgt gcacgcggcc
     1921 tcgggtgcga ccgaatcgat gtagcaggtc agggtttcgc ccgtggtgcc ctcgatatcg
     1981 atcgagatgt cttcaccacg cgcggccagg accgcatcgt tgatcgccgc gtaccgaacg
     2041 ttgtcgctca tccaggcgcc ctggaaggtc tccatcgtca cggtaggcag aatgccggtc
     2101 atgacgatgt gggtgcccac ctcggacgag cgttgctcgg ccgcgttcat cgaggctcgc
     2161 agggcctgct cctgatcggc cagtgccgcg cccgagaggc gtccgggcgg cacgttgaac
     2221 tcgatgttga agcggcccaa ctcggtttga tactcgggat cggcgatggc ggcgagcacg
     2281 tcggcgttgt ccatggccgg gcgctggtcg gcgtcgacca ggttgagctc gatctccatg
     2341 ccgaccagcg gctcgtcgaa ctcgaagctg tgcgcggaga gcatctgctc gaagacgtcc
     2401 aagcagcgct gcaccttctc ccggtaacgc tggcgctgct cgcgggtgta cgttcgcgcg
     2461 gcgatgtcac ggcccatggc ggggagtatg gcgcacggcg ccagtggatg cacgagtacg
     2521 gggcctcgaa cgtgccgaca tcgtcatggc gcggctctgg ctctggtgcg aagccgctgc
     2581 gggcgctatc tttgttcgat attatgaacg ctgttcttta gagcgaatgg agcgggcatt
     2641 gtcgcctgta cgcggaggaa ccgacgtcac gccacccggc gcggcaccgg cggtcagccg
     2701 gggcatcgcc gtgctggacg ccctggccgg aagcgagaac gggtcgatga cgctgtccga
     2761 cctcagccgc gccctcgggg tgcccaaatc gtccaccagc gccatttgta ccgcactcga
     2821 actcgggggc atggtgcgcc gcgacgacct cggctattcc ctcggacgca agctggtcga
     2881 actcgggggt gcatatctcg cgcggctcga ccaggtcagc gagttccatc gcgcctgcct
     2941 cgagtcgacc gtctttcgcg ccgagaccct gcgcctctca acggcggccg gcatcgactg
     3001 cctgtgcctg gcccgctacg aggggcaccc cgcgatccgg ctcaccacca acatcggcga
     3061 tcggctgccg gcctcgtcat gtgcgcaggg caaggcgctg ctcgcgcggc tggacgactc
     3121 cgagatcgaa cggctctatc acggcatcga cgaactgccg cgactgacca gccggtcacg
     3181 gcgcagcgtg cccaggctgc tgaaggacat ccggctggcc cgggccaggg ggtacgcgat
     3241 cgacgaggag gaatccgccg acagcgtgat gggcctggcc gtggtggtgc cgacccgcgg
     3301 cgtgcgggcc ccgatcctcg cgatcagcgt gaccaagcac aagtcggatt ggaacgagcc
     3361 gacccgcgaa cggctggtcg acgagctgaa acgcgtggcg acgatgctcg gcaacccgat
     3421 ggcaaccgga taggacgtcg ctcgcagttc ccgccgtccc ggtctccgtc gtgctgctgt
     3481 ccgggcccca tgttgtgccg ccggtgcgtt tcgctccacc accctcgcgt ccacacgccc
     3541 gagatccaca cagtgctcat gtagagctgt gaaacggacg atcccccacc tttcgcgtgc
     3601 accacccctg tgatacacgc cgccgcacag gcagtcacgt cgcagacaca ccccggcccc
     3661 cgagcgtccg ccttgccgcc aaaggtcagt tggtcgcaga gtctcggttg ttcggtctat
     3721 tgaccatcgt tcgatctatg gtgcatactg atcgcagcct cacagagaac aaaggagtct
     3781 cgatgtctga gtcacacgcc atacacggca ccagcaatga gcacgaggtc gtcgaccccg
     3841 tccaacttcg ccgcgccacc ctcgcctcgt cggtgggctc ggccctggag tactacgact
     3901 tctatatcta cggtctcgcc agcgccttga tcttcggcga gctcttcttc aagccgctcg
     3961 gtgccgccgg tgcgctgatc gcggccttcg gcacctacgc ggtcggcttc gccgctcgtc
     4021 ccgtcggcgg cctggttttc ggccacctcg gcgacaagct cggccgcaag ctgatcctga
     4081 tcatcacgat cgcgctgatg ggtggctcgt cgatgctgat cggcctgctg cccacctatg
     4141 accaggtcgg catgtggggc gccattctgc tgatccttct gcgcatcgcg cagggcctgg
     4201 gcgccggtgc cgaacaggcc ggtgccaccg tactgatctc ggagttcgc
//
LOCUS       DAOSUG010000247         4200 bp    DNA     linear   ENV 10-JUN-2024
DEFINITION  MAG TPA_asm: Micropruina sp. isolate AMR_MDS_2195
            NODE_8934_length_4200_cov_4.137033, whole genome shotgun sequence.
ACCESSION   DAOSUG010000247 DAOSUG010000000
VERSION     DAOSUG010000247.1
DBLINK      BioProject: PRJNA925477
            BioSample: SAMN32864262
            Sequence Read Archive: SRR4236673
KEYWORDS    WGS; ENV; Metagenome Assembled Genome; MAG; Third Party Data; TPA;
            TPA:assembly.
SOURCE      Micropruina sp. (wastewater metagenome)
  ORGANISM  Micropruina sp.
            Bacteria; Bacillati; Actinomycetota; Actinomycetes;
            Propionibacteriales; Nocardioidaceae; Micropruina.
REFERENCE   1  (bases 1 to 4200)
  AUTHORS   Abdulkadir,N., Saraiva,J.P., Zhang,J., Stolte,S., Gillor,O.,
            Harms,H. and Rocha,U.
  TITLE     Genome-centric analyses of 165 metagenomes show that mobile genetic
            elements are crucial for the transmission of antimicrobial
            resistance genes to pathogens in activated sludge and wastewater
  JOURNAL   Microbiol Spectr 12 (3), e0291823 (2024)
   PUBMED   38289113
REFERENCE   2  (bases 1 to 4200)
  AUTHORS   Abdulkadir,N.
  TITLE     Direct Submission
  JOURNAL   Submitted (24-MAR-2023) Environmental Microbiology, Helmholtz
            Center for Environmental Research -UFZ, Permoserstr 15, Leipzig
            04318, Germany
COMMENT     The annotation was added by the NCBI Prokaryotic Genome Annotation
            Pipeline (PGAP). Information about PGAP can be found here:
            https://www.ncbi.nlm.nih.gov/genome/annotation_prok/
            
            ##Genome-Assembly-Data-START##
            Assembly Method        :: SPAdes v. 3.11.1
            Genome Representation  :: Full
            Expected Final Version :: Yes
            Genome Coverage        :: Not Applicable
            Sequencing Technology  :: Illumina MiSeq
            ##Genome-Assembly-Data-END##
            
            ##Genome-Annotation-Data-START##
            Annotation Provider               :: NCBI
            Annotation Date                   :: 05/04/2023 04:57:10
            Annotation Pipeline               :: NCBI Prokaryotic Genome
                                                 Annotation Pipeline (PGAP)
            Annotation Method                 :: Best-placed reference protein
                                                 set; GeneMarkS-2+
            Annotation Software revision      :: 6.5
            Features Annotated                :: Gene; CDS; rRNA; tRNA; ncRNA
            Genes (total)                     :: 2,776
            CDSs (total)                      :: 2,750
            Genes (coding)                    :: 2,714
            CDSs (with protein)               :: 2,714
            Genes (RNA)                       :: 26
            tRNAs                             :: 24
            ncRNAs                            :: 2
            Pseudo Genes (total)              :: 36
            CDSs (without protein)            :: 36
            Pseudo Genes (ambiguous residues) :: 0 of 36
            Pseudo Genes (frameshifted)       :: 2 of 36
            Pseudo Genes (incomplete)         :: 32 of 36
            Pseudo Genes (internal stop)      :: 2 of 36
            ##Genome-Annotation-Data-END##
FEATURES             Location/Qualifiers
     source          1..4200
                     /organism="Micropruina sp."
                     /mol_type="genomic DNA"
                     /submitter_seqid="NODE_8934_length_4200_cov_4.137033"
                     /isolate="AMR_MDS_2195"
                     /isolation_source="Activated sludge of municipal
                     wastewater treatment plants"
                     /db_xref="taxon:2737536"
                     /environmental_sample
                     /geo_loc_name="Canada"
                     /lat_lon="49.25 N 123.1 W"
                     /collection_date="2015-06-16"
                     /metagenome_source="wastewater metagenome"
                     /note="metagenomic"
     gene            complement(248..649)
                     /locus_tag="PLF56_08750"
     CDS             complement(248..649)
                     /locus_tag="PLF56_08750"
                     /inference="COORDINATES: ab initio
                     prediction:GeneMarkS-2+"
                     /note="Derived by automated computational analysis using
                     gene prediction method: GeneMarkS-2+."
                     /codon_start=1
                     /transl_table=11
                     /product="hypothetical protein"
                     /protein_id="HOQ53686.1"
                     /translation="MSSQPTIPQYIPRDDEDLTPAPENVEEDNEGGLVIDDTPVIDAT
                     QVDPAAIHDTSAPAADSDQGSSTVADPDQGSPTVAHSGQGSPTVADPDQGSTVPDEGA
                     AASRSDVPVEPIPPVSDPKDDPVVAAAENKQ"
     gene            complement(745..1944)
                     /locus_tag="PLF56_08755"
     CDS             complement(745..1944)
                     /locus_tag="PLF56_08755"
                     /inference="COORDINATES: protein motif:HMM:NF012607.2"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_function: GO:0051287 - NAD binding [Evidence IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="NAD(P)-dependent oxidoreductase"
                     /protein_id="HOQ53687.1"
                     /translation="MRVLVADSFESSGLKALRAKECEVVHEPGLKDNALVARVADLAP
                     DVLIVRSTKVPEAVLAAAPLKLVVRAGAGYNTIDVAAASRRGIYVSNCPGKNSIAVAE
                     LAFALIAALDRRIADNVADLRAGVWNKSEYGKAKGLYGRTLGLIGLGGIGSAMVPRAQ
                     AFGMPVVAWSRSLTDERAAELGITRLDSITDVARAADMVSVHLALNDDTRGLLGAEFF
                     AKLREGAYFINTSRGEVIDEASLADAVRDKGVRVGLDVFANEPGGGSGEFHPAIVDLP
                     GVYGTHHIGASTDQAQRAIADEAVRIVTVFAETGRVPNVVNLAEATPATSTLVIRHLD
                     RPGVLAAALDAISDAGLNVQEMENVVFSGGEAAVARINLEGTVSPETMAAIGAHESVL
                     DVQLIAL"
     gene            2152..3105
                     /locus_tag="PLF56_08760"
     CDS             2152..3105
                     /locus_tag="PLF56_08760"
                     /inference="COORDINATES: ab initio
                     prediction:GeneMarkS-2+"
                     /note="Derived by automated computational analysis using
                     gene prediction method: GeneMarkS-2+."
                     /codon_start=1
                     /transl_table=11
                     /product="hypothetical protein"
                     /protein_id="HOQ53688.1"
                     /translation="MDKKAIDATSRRLRELLLDKQYADRWRPYVGRRQAHALHQSSIC
                     HVIADYLVDNQLQPDDDDLARKLKDRVHRALAGQSLSLGTLRWFIEAFEMTPHDTADL
                     LALAGPKAPNCTVIGGSVVGRGTLPQRHHHTVSLHEWHRVGPDGLPESHRTVHVIRAN
                     DRLERYPYIFDTDAASVRVVQGGRAGQPYALDNGLYAVDIVLTRPLSPGETASMEYET
                     TFWYRTPPPPEFRRASSGRIDNIDLRVQFHHLRLPVQVWWSEWPALDAAPALNEPLRL
                     NADKSVHRYLDSVENTVVGFRWEIAQHRRRPNAVVAGRLFP"
     gene            complement(3366..3764)
                     /locus_tag="PLF56_08765"
     CDS             complement(3366..3764)
                     /locus_tag="PLF56_08765"
                     /inference="COORDINATES: ab initio
                     prediction:GeneMarkS-2+"
                     /note="Derived by automated computational analysis using
                     gene prediction method: GeneMarkS-2+."
                     /codon_start=1
                     /transl_table=11
                     /product="hypothetical protein"
                     /protein_id="HOQ53689.1"
                     /translation="MLFAPQTPLETLLVQAYGRPTDARAYTAFVRELMSAVVGVLGRR
                     EPHGFQPLVLSPAGTAGVCVFTHPARYDTFAAELMLPSADWQVRSERVRPLFEWAAVN
                     RLYVLLNPGSDPAKEFPAVEVERLLRGQWL"
     gene            complement(3767..4198)
                     /locus_tag="PLF56_08770"
     CDS             complement(3767..4198)
                     /locus_tag="PLF56_08770"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_014846011.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="NUDIX domain-containing protein"
                     /protein_id="HOQ53690.1"
                     /translation="MLWLSGATAVIVRPGPHGDEVLLVKRSDDGNWSPIAGIVDPGED
                     PHMTAVREAAEEACVEVEVQRLAWLNVTDPVSYPNGDVTQYIDHTFRCRWVAGEPRPG
                     DGEATDARFFSVDGLPDMPQHYLQRIEVALQDRPDTVLGKL"
BASE COUNT          678 a         1447 c         1393 g          682 t
ORIGIN      
        1 cacgacagtt accacgtcgc gacgctcgac aatgacgcgg agttggtctt cgacgagtcg
       61 gtgacgttca tccgccgggt ggccggcgag ggctgagtac gtccgccgac tccgacgcca
      121 ccgggcgtgg tcgcagcccg aacgagtacg ggttcgacag ccaggctcga acgcgcccag
      181 gagctgtgga cggttccgtg agcaggagct ggggacggtt cgtgcccagc tcctactcag
      241 cggctcgtta ctgcttgttc tcggcggcgg cgacgaccgg gtcgtccttg gggtcgctga
      301 ccggcgggat cggctcgacc ggcacgtccg atcgggacgc cgcggccccc tcgtcaggga
      361 ccgtggagcc ttggtcaggg tctgcgaccg tgggcgagcc ttggccagag tgtgcgaccg
      421 tgggcgagcc ttggtcaggg tctgcgaccg tggacgagcc ttggtcggag tccgcggcag
      481 gggccgaggt gtcatggatc gcagcggggt cgacctgcgt ggcgtcgatg acgggggtgt
      541 cgtcgatcac caggccgccc tcgttgtcct cctcgacgtt ctcgggtgcc ggggtcagat
      601 cctcatcgtc gcgaggaatg tactgcggaa tggtcggttg gctgctcatc ggtgcctcct
      661 tgcagtgaga gtcccgattc ttgccccgcg tccaccccgt ggcaatcccc tttgtgggtg
      721 gactcgtgcg cgacccggca ccactcacaa cgcgatcagc tgcacgtcca gcacactctc
      781 gtgggcgccg attgctgcca tcgtttctgg cgacaccgtg ccctcgagat tgatccgggc
      841 cacggcagcc tcgccgccgg agaagaccac gttctccatc tcttgcacgt tgagcccggc
      901 gtcgctgatc gcgtcgaggg ccgccgcgag cacgcccggc cggtcgaggt gtcggatcac
      961 cagggtggac gtggccggcg tggcctcggc caggttcacc acgttcggca cccgtccggt
     1021 ttcggcgaag accgtgacga tgcgcaccgc ctcgtcggcg atcgcgcgtt gcgcctgatc
     1081 ggtggacgcg ccgatgtgat gggtgccgta caccccgggc aggtcgacga tggccgggtg
     1141 gaactcgccg ctgccgccgc cgggttcgtt ggcgaagacg tccaggccga cccggacgcc
     1201 cttgtcgcgc accgcgtcgg cgagcgaagc ctcatcgatc acctcaccgc gggaggtgtt
     1261 gatgaagtag gcaccctcgc gcagtttggc gaagaactcg gcacccagca gcccccgggt
     1321 gtcatcgttg agggccaggt gcacgctgac catgtctgcg gcgcgggcca cgtccgtgat
     1381 ggagtcgagc cgcgtgatgc cgagttcggc ggcgcgctcg tcggtcaggc tgcgactcca
     1441 ggccaccacc ggcatgccga atgcctgcgc ccgcggcacc atggccgacc cgatgccgcc
     1501 caggccgatc aggcccaggg tgcgtccgta caggcccttc gccttgccgt actcgctctt
     1561 gttccacacc ccggcgcgca ggtcggcgac gttgtccgcg atccgacggt cgagcgcggc
     1621 gatcagcgcg aacgccaact cggccaccgc gatggagttc ttgccgggac agttcgacac
     1681 gtagatgccg cgcctggacg ccgccgccac gtcgatggtg ttgtagccgg ccccggcccg
     1741 caccacgagc ttcagcggcg cggccgccag caccgcctcc ggcaccttgg tgctgcgcac
     1801 gatcaacaca tcaggggcca ggtcggcgac ccgggcgacc agtgcgttat ccttcaaacc
     1861 cggctcgtgc acgacctcgc attccttggc ccgcagggcc ttcagacccg aggattcaaa
     1921 actgtcggcc accagtacgc gcatgcagtc aacttactgg tgtcgcgcct tcgacgatgc
     1981 tcctcgtgac gaggtaccgg ttgagcgagc cgaggcccca gctcgccggg gtctcgacgg
     2041 gcgcgaccag ctggttcagc tcgggtgagg cccacctggc gtgacggaag tggcacgggt
     2101 gtgcttttgc atcatttagg atgccaatag gacacccccg aagaaggacc gttggacaag
     2161 aaggcaattg acgccacgtc ccgcagattg cgcgagttgt tgctcgacaa gcaatatgcg
     2221 gatcgttggc gaccctatgt cgggcgccgg caggcgcacg ctctgcatca gagcagcatc
     2281 tgccacgtga tcgccgacta tctggtcgat aaccagcttc agcccgatga cgacgacctg
     2341 gctcgcaagc tgaaggaccg ggtgcaccgc gctctcgccg gccagtcgct cagcctcggc
     2401 accctgcgct ggttcatcga agccttcgag atgacaccgc acgacaccgc ggacctgttg
     2461 gcgctcgccg ggccgaaggc ccccaactgc acggtgatcg gcggttctgt ggtcggccgg
     2521 ggaaccctgc cgcagcggca ccatcacacc gtgtccctgc acgaatggca ccgggtggga
     2581 ccagacggcc taccggaatc gcaccgcacc gtgcatgtga ttcgggccaa cgaccgcctg
     2641 gagcgctacc cctacatctt cgacaccgac gcggccagcg tgcgcgtcgt ccagggtgga
     2701 cgggccggcc aaccctacgc cctcgacaac ggcctctacg ccgtcgacat cgtgctcacc
     2761 cggccgctct cgccgggcga aaccgcgtcc atggaatacg aaaccacctt ctggtaccgc
     2821 accccgccgc cgccggagtt ccggcgggcc agcagcggcc ggatcgacaa catcgacctg
     2881 cgggtgcagt ttcatcacct gcggctgccc gtgcaggtgt ggtggagcga gtggccggcc
     2941 ctggacgctg cgccggcgtt gaacgagccg ctcaggctca atgccgacaa gagcgtgcac
     3001 cgctacctgg actcggtgga gaacacggtg gtgggtttcc gctgggagat agcgcagcat
     3061 cgacgccgcc cgaacgcggt ggtcgccggg cgcctgtttc cctgaccggg cgttgagtgc
     3121 cggacgaagg agaacctggg cctggggctc agccggtgat cagactcgcc agtaacatca
     3181 gggccgcaac ggtggtggca accgccaggg cgacgacgcg aaccagcgag tgaccggggg
     3241 tgggtagcac gctcatgccc tcattaacgg cccgcggcgc cacaacgttg cgctcgacac
     3301 ggtcatttct caccagttgg cccgatttca cccttaaggt tgctcgcgcc gatgcttgag
     3361 acgattcaca gccattgccc ccgcaacaag cgctcgactt caacagcagg aaactctttt
     3421 gcgggatcac tgccaggatt gagcaacacg taaagccggt tcaccgccgc ccactcgaac
     3481 aacggccgca cccgctcaga gcgcacctgc cagtcggcgg acggcagcat gagctcggcg
     3541 gcgaaggtgt cgtatcgcgc cggatgggtg aaaacgcaca ccccggcggt gccggccggc
     3601 gacagcacca gaggctgaaa accgtgcggc tcgcgtcgtc cgagcacccc caccaccgcg
     3661 ctcatcaact cacgcacgaa ggcggtgtag gcgcgggcgt cggtcgggcg cccataggcc
     3721 tggaccagca acgtctccag cggagtctgt ggcgcgaaca gcatgattac agtttgccga
     3781 gcacggtgtc gggccggtcc tgcagcgcga cctcgatgcg ttgcaaatag tgctgcggca
     3841 tgtcgggcag cccgtcgacg gagaagaatc gcgcgtcggt cgcctccccg tcacccggac
     3901 gcggctcgcc cgcgacccag cggcagcgaa acgtgtggtc gatgtactgc gtgacgtccc
     3961 cgttggggta gctgaccggg tcggtgacgt tcagccaggc cagccgctgc acctccacct
     4021 cgacgcaggc ctcttcggcc gcctcgcgca cggcggtcat gtgcgggtcc tcgcccggat
     4081 cgacgatgcc ggcgatcggc gaccagttgc cgtcgtccga ccgcttcacc agcagcacct
     4141 cgtcaccgtg cggccccggc cgcacgatca cggctgtcgc tcccgacagc cagagcatct
//
LOCUS       DAOSUG010000248         4178 bp    DNA     linear   ENV 10-JUN-2024
DEFINITION  MAG TPA_asm: Micropruina sp. isolate AMR_MDS_2195
            NODE_8979_length_4178_cov_4.966287, whole genome shotgun sequence.
ACCESSION   DAOSUG010000248 DAOSUG010000000
VERSION     DAOSUG010000248.1
DBLINK      BioProject: PRJNA925477
            BioSample: SAMN32864262
            Sequence Read Archive: SRR4236673
KEYWORDS    WGS; ENV; Metagenome Assembled Genome; MAG; Third Party Data; TPA;
            TPA:assembly.
SOURCE      Micropruina sp. (wastewater metagenome)
  ORGANISM  Micropruina sp.
            Bacteria; Bacillati; Actinomycetota; Actinomycetes;
            Propionibacteriales; Nocardioidaceae; Micropruina.
REFERENCE   1  (bases 1 to 4178)
  AUTHORS   Abdulkadir,N., Saraiva,J.P., Zhang,J., Stolte,S., Gillor,O.,
            Harms,H. and Rocha,U.
  TITLE     Genome-centric analyses of 165 metagenomes show that mobile genetic
            elements are crucial for the transmission of antimicrobial
            resistance genes to pathogens in activated sludge and wastewater
  JOURNAL   Microbiol Spectr 12 (3), e0291823 (2024)
   PUBMED   38289113
REFERENCE   2  (bases 1 to 4178)
  AUTHORS   Abdulkadir,N.
  TITLE     Direct Submission
  JOURNAL   Submitted (24-MAR-2023) Environmental Microbiology, Helmholtz
            Center for Environmental Research -UFZ, Permoserstr 15, Leipzig
            04318, Germany
COMMENT     The annotation was added by the NCBI Prokaryotic Genome Annotation
            Pipeline (PGAP). Information about PGAP can be found here:
            https://www.ncbi.nlm.nih.gov/genome/annotation_prok/
            
            ##Genome-Assembly-Data-START##
            Assembly Method        :: SPAdes v. 3.11.1
            Genome Representation  :: Full
            Expected Final Version :: Yes
            Genome Coverage        :: Not Applicable
            Sequencing Technology  :: Illumina MiSeq
            ##Genome-Assembly-Data-END##
            
            ##Genome-Annotation-Data-START##
            Annotation Provider               :: NCBI
            Annotation Date                   :: 05/04/2023 04:57:10
            Annotation Pipeline               :: NCBI Prokaryotic Genome
                                                 Annotation Pipeline (PGAP)
            Annotation Method                 :: Best-placed reference protein
                                                 set; GeneMarkS-2+
            Annotation Software revision      :: 6.5
            Features Annotated                :: Gene; CDS; rRNA; tRNA; ncRNA
            Genes (total)                     :: 2,776
            CDSs (total)                      :: 2,750
            Genes (coding)                    :: 2,714
            CDSs (with protein)               :: 2,714
            Genes (RNA)                       :: 26
            tRNAs                             :: 24
            ncRNAs                            :: 2
            Pseudo Genes (total)              :: 36
            CDSs (without protein)            :: 36
            Pseudo Genes (ambiguous residues) :: 0 of 36
            Pseudo Genes (frameshifted)       :: 2 of 36
            Pseudo Genes (incomplete)         :: 32 of 36
            Pseudo Genes (internal stop)      :: 2 of 36
            ##Genome-Annotation-Data-END##
FEATURES             Location/Qualifiers
     source          1..4178
                     /organism="Micropruina sp."
                     /mol_type="genomic DNA"
                     /submitter_seqid="NODE_8979_length_4178_cov_4.966287"
                     /isolate="AMR_MDS_2195"
                     /isolation_source="Activated sludge of municipal
                     wastewater treatment plants"
                     /db_xref="taxon:2737536"
                     /environmental_sample
                     /geo_loc_name="Canada"
                     /lat_lon="49.25 N 123.1 W"
                     /collection_date="2015-06-16"
                     /metagenome_source="wastewater metagenome"
                     /note="metagenomic"
     gene            complement(197..520)
                     /locus_tag="PLF56_08775"
     CDS             complement(197..520)
                     /locus_tag="PLF56_08775"
                     /inference="COORDINATES: protein motif:HMM:NF023257.2"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="DUF3349 domain-containing protein"
                     /protein_id="HOQ53691.1"
                     /translation="MLSRIERIVNWLKGGYPQGIPEADYVPLLAVLRRRLSPDEIDEL
                     AQRIVRDGLIPAERVDLGAEFLRVTDELPSEAELQRISEKLNASGVDVSYQPPAWVRP
                     DPGNA"
     gene            complement(530..859)
                     /locus_tag="PLF56_08780"
     CDS             complement(530..859)
                     /locus_tag="PLF56_08780"
                     /inference="COORDINATES: protein motif:HMM:NF023257.2"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="DUF3349 domain-containing protein"
                     /protein_id="HOQ53692.1"
                     /translation="MSDHKSPFERILHWLRAGYPEGVPQHDYVALLGVLQRSLTTDEV
                     EQLALRLREERGSSTSDEQIRDLIRQTALEEPSEADVRRVASRLAQGGWPLEHPATAT
                     AATSQEG"
     gene            complement(874..1164)
                     /locus_tag="PLF56_08785"
     CDS             complement(874..1164)
                     /locus_tag="PLF56_08785"
                     /inference="COORDINATES: ab initio
                     prediction:GeneMarkS-2+"
                     /note="Derived by automated computational analysis using
                     gene prediction method: GeneMarkS-2+."
                     /codon_start=1
                     /transl_table=11
                     /product="hypothetical protein"
                     /protein_id="HOQ53693.1"
                     /translation="MFQLFGNAIGQVVAVSVLVGAGLPALFAFGIRALAAGEGGSAEV
                     SGVAGKPAMKVLAWVVFALVLAVIAVGLAIIVSSGFGYKVSFDHIFPTFIKK"
     gene            complement(1178..2410)
                     /locus_tag="PLF56_08790"
     CDS             complement(1178..2410)
                     /locus_tag="PLF56_08790"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_013863866.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_component: GO:0016020 - membrane [Evidence IEA];
                     GO_process: GO:0006817 - phosphate ion transport [Evidence
                     IEA];
                     GO_process: GO:0055085 - transmembrane transport [Evidence
                     IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="inorganic phosphate transporter"
                     /protein_id="HOQ53694.1"
                     /translation="MNLELLLLILVIGTAIAFDFTNGFHDTGNAMATSIATGALKPKV
                     AVGLSAILNLVGAFLSVEVALTVTNAVVKLQNSSGAPNPELLVNNGHELLMIVFCGLI
                     GGILWNLVTWLLGLPSSSSHALFGGLIGATAAALGLGGVNWMGDGTKLDGVLGKVILP
                     AFASPVVAALVATIGTWLVFKITNGVAERYAETGFRWGQIGTASLVSLAHGTNDAQKT
                     MGVITLALIAYGVQTDTTSVPLWVKFACAFAIAAGTYTGGWRIIRTLGKGLVEISSPQ
                     GMAAEASSAAVILTSSHLGFALSTTHVATGSILGSGLGKKGAAVRWGVAMRMVLAWFI
                     TLPAAGIVGALCFWLGNGIGGYAGPLLVFAILLGLAGWMIMHSRKTAVDANNVNDEWH
                     PDATPGASVAPETAAIKA"
     gene            complement(2865..3893)
                     /gene="ilvC"
                     /locus_tag="PLF56_08795"
     CDS             complement(2865..3893)
                     /gene="ilvC"
                     /locus_tag="PLF56_08795"
                     /EC_number="1.1.1.86"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_002526599.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="ketol-acid reductoisomerase"
                     /protein_id="HOQ53695.1"
                     /translation="MAEMFYDDDADLSIIQNRVVAVIGYGSQGHAHALSLRDSGVDVR
                     VGLRPGSKSAAKAEAEGLRVLSVADAAAEADVVMILAPDQFQRTIYAEDIAPNLQEGD
                     ALFFAHGFNIRFGYITAPAGVDVCMVAPKGPGHLVRREYSEGRGVPVIVAVEVDASGQ
                     AWPLALSYAKAIGGLRAGGIKTTFTEETETDLFGEQAVLCGGVSKLVQTGFEVLTEAG
                     YQPEVAYFECLHELKLIVDLMYEGGIAKQRWSVSDTAEFGDYVSGPRVIDAAVKKNMQ
                     DVLADVQSGAFAKRFIDDQDAGAPEFAKLRAEGEAHPIEATGRELRGLMAWVKSHDDD
                     YVEGTAAR"
     gene            complement(3908..>4178)
                     /gene="ilvN"
                     /locus_tag="PLF56_08800"
     CDS             complement(3908..>4178)
                     /gene="ilvN"
                     /locus_tag="PLF56_08800"
                     /EC_number="2.2.1.6"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_016455672.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_component: GO:0005948 - acetolactate synthase complex
                     [Evidence IEA];
                     GO_function: GO:0003984 - acetolactate synthase activity
                     [Evidence IEA];
                     GO_process: GO:0009097 - isoleucine biosynthetic process
                     [Evidence IEA];
                     GO_process: GO:0009099 - valine biosynthetic process
                     [Evidence IEA]"
                     /codon_start=2
                     /transl_table=11
                     /product="acetolactate synthase small subunit"
                     /protein_id="HOQ53696.1"
                     /translation="IKLKADPSTRSQIIEIVQLFRGKTVDVQNDSLTIEATGSPDKLE
                     ALLEMLRPYGVRELVQSGLVALGRGSKSLTDRAKTDRVRTPVRNN"
BASE COUNT          700 a         1412 c         1381 g          685 t
ORIGIN      
        1 gagcgggcgt actgggtgcg ccgcagcgcg gtcagggtta tgcgacacgc cctaggtgca
       61 caccggtcgt ccggcgaacg tggctcacgt cggcgcccac tctgagccgg tccgcccggc
      121 ccgcccagcc cccgcccaac tatctcgggc ctgcgaagat tcgcaggccc gagatagtta
      181 ggcgggggct cggggctcag gcgttgccgg ggtcggggcg gacccaggcc ggcggctgat
      241 agctgacatc gacacccgag gcgttcagct tctcgctgat gcgttgcagt tctgcctcgc
      301 tgggcaactc gtcggtgacg cgcagaaact ccgccccgag atcgacccgc tcggccggta
      361 tcaggccgtc acggacgatg cgctgcgcga gttcgtcgat ctcgtccggg ctcagcctgc
      421 gtcggagcac cgccagtagc gggacgtagt ccgcctcggg gatgccctgc gggtagcccc
      481 ccttgagcca gttgacgatg cgctcgatcc gcgacagcac tgctcggcct caaccctcct
      541 ggctcgtcgc cgcagtggcg gttgccgggt gctccagcgg ccagccgccc tgggccaatc
      601 ggctcgcgac gcgccgaacg tcggcctcgg agggttcctc cagggccgtt tgcctgatca
      661 gatccctgat ctgttcgtcg ctggtggacg aaccacgctc ttcacgaagc cgcaacgcca
      721 gctgctccac ctcgtcggtg gtcagcgaac gctgcagaac gcccagcaga gccacgtaat
      781 cgtgctgcgg gacgccttcg gggtagccgg cgcgcagcca gtgcagaatc cgctcgaacg
      841 ggctcttgtg gtctgacatc gtgtgcgccg ctctcacttc ttgatgaacg tcgggaaaat
      901 gtggtcgaac gataccttat agccgaaccc cgacgacacg atgatggcga gtccgacggc
      961 gatcacggcc agcacgagtg cgaacacgac ccaggccaga accttcatgg cgggcttgcc
     1021 tgccacaccg ctgacttcgg ccgagccgcc ctcgcctgcg gcgagcgcgc gaataccgaa
     1081 ggcgaacagg gcgggcaggc cggcgccgac gagaacagag acggccacga cctggccgat
     1141 cgcatttccg aaaagctgaa acatgtcgtt gccttcctca ggccttgatg gcggcggtct
     1201 cgggggcgac ggaggcgccc ggcgtggcgt cgggatgcca ttcgtcgttg acgttgttgg
     1261 catccaccgc cgtcttgcgt gagtgcatga tcatccagcc ggccaggccg agcaggatcg
     1321 cgaacaccaa aagtggcccg gcgtacccgc cgatgccgtt gccgagccag aagcacaggg
     1381 cgcccacgat gccggcggca ggcagggtga tgaaccacgc cagcaccatg cgcatcgcca
     1441 caccccaacg cacggccgcg cccttcttgc ccaggccgga gcccagaatc gagccggtcg
     1501 cgacgtgggt ggtcgacagt gcgaaaccca gatggctcga ggtcaggatc acggcggcgg
     1561 acgatgcctc ggcagccatg ccctgcggcg aactgatctc gaccaggccc ttgccgaggg
     1621 tacggatgat gcgccagccg ccggtgtagg tgccggccgc gatggcgaag gcacaggcga
     1681 acttgaccca caacggcacg ctggtggtgt cggtctgcac gccgtacgcg atcagggcga
     1741 gtgtgatcac gcccatcgtc ttctgggcgt cgttggtgcc gtgggccagc gagaccagcg
     1801 aggcggtccc gatctggccc cagcgaaagc cggtctcggc ataacgctcg gccaccccgt
     1861 tggtgatctt gaacaccagc caggtgccga tcgtggcgac cagggccgcg accaccggcg
     1921 aagcgaacgc cggcaggatc accttgccca gcaccccgtc cagcttggtg ccgtcaccca
     1981 tccagttgac cccgcccagg ccgagggccg cggcggtggc gccgatcagg ccgccgaaga
     2041 gggcgtggga actgctggac ggcagcccga gcagccaggt gacgaggttc cacagaatgc
     2101 caccgatgag tccacaaaag acgatcatca gcagttcgtg gccgttgttg acgagcagtt
     2161 cggggttggg tgcaccgctg gagttctgca gtttcaccac ggcgttggtg acggtgagcg
     2221 ccacctcgac cgacaggaag gcaccgacca ggttgaggat cgcggacagc cccaccgcaa
     2281 ccttgggttt gagcgcgccg gtggcgatcg aggtcgccat cgcgttgccc gtgtcgtgaa
     2341 agccattggt gaaatcgaag gcaatggctg tgccgatcac gaggatcagc agcaacagtt
     2401 ctaggttcac cctcgaaggg tgggctttcg gagtgaacag atggtccgca caaggtgaac
     2461 agacggtgac cgccatgcag cagtcagccg aatctttggg cgcctctcag gatcgcacca
     2521 cagctcggtc aagcgaagct gagtaggtac gggtggcggc ttctgatccg agcccagaac
     2581 ggtacgagta cggagatcac gtcgaagatc agctgagtgt gggactgttc ctcacgtggt
     2641 tgacggccgc cccctcccaa cccaaccgcc tccctgacac gtggctgcct gacacgtagc
     2701 tgcctacgcg caggcagtca ctcgtcaccg aggcagtcac gtcgaactgg cagatcgcgc
     2761 gctgagtcca acaccgtgct tgaaaaggcg caacgggcgc ccgtccctta tggggacgga
     2821 cgcccgtttc tcgtgcgtcc cggggtggcg ggacgcaggc tcactcagcg ggcggcagtg
     2881 ccctcgacgt agtcgtcgtc gtggctcttc acccaggcca tcaggccgcg cagttcgcgt
     2941 ccggtggcct cgatcgggtg cgcttcgccc tcggcgcgca gcttggcgaa ctcgggcgcg
     3001 ccggcgtcct ggtcgtcgat gaaccgcttc gcgaacgccc cggactggac gtcggccagc
     3061 acgtcctgca tgttcttctt caccgcggcg tcgatcaccc gcggacccga cacgtagtcg
     3121 ccgaactcgg cggtgtccga gaccgaccag cgctgcttgg cgatgccgcc ctcgtacatc
     3181 aggtcgacga tcagcttcag ttcgtgcagg cactcgaagt aggccacctc tggctggtag
     3241 ccggcctcgg tcagcacctc gaagccggtc tgcaccagct tggagacacc accgcacagc
     3301 accgcctgct cgccgaacag gtcggtctcg gtctcttcgg tgaaggtggt cttgatgccg
     3361 ccggctcgca gtccgccgat cgccttggcg tagctgagcg ccagcggcca ggcctggccg
     3421 gaggcgtcca cctcgacggc gacgatcacc ggcacgccgc gaccctcgga gtactcccgg
     3481 cgcaccaggt gcccgggccc cttgggggcg accatgcaga cgtccacgcc ggcgggagcg
     3541 gtgatgtagc cgaagcggat gttgaagccg tgggcgaaga acagcgcgtc gccctcttgc
     3601 aggttgggcg cgatgtcttc ggcgtagatg gtgcgctgga actggtcggg cgccaggatc
     3661 atgaccacgt cggcctcggc ggcggcgtcc gccaccgaca gcacccgcag cccctcggcc
     3721 tcggccttgg ccgccgactt cgagcccgga cgcagcccca cccgcacgtc gacgccggag
     3781 tcgcgcagcg acaatgcgtg cgcgtggccc tgcgagccgt agccgatcac cgcgaccacc
     3841 cggttctgaa tgatcgaaag gtccgcgtcg tcgtcgtaga acatctctgc cataacttct
     3901 tccttaccta gttgtttcgc acaggtgtgc ggactctgtc tgtcttggcc cggtcggtca
     3961 gcgacttgct gccccggccc agcgccacca gcccggactg gacgagctcg cgcacgccgt
     4021 agggtcgcag catctcgagc agtgcctcca gcttgtcggg cgatccggtg gcctcgatgg
     4081 tcaacgagtc gttctgcacg tccacggtct tgccccggaa caactggacg atctcgatga
     4141 tctggctgcg cgtgctgggg tcggccttca gcttgatc
//
LOCUS       DAOSUG010000249         4174 bp    DNA     linear   ENV 10-JUN-2024
DEFINITION  MAG TPA_asm: Micropruina sp. isolate AMR_MDS_2195
            NODE_8990_length_4174_cov_4.632435, whole genome shotgun sequence.
ACCESSION   DAOSUG010000249 DAOSUG010000000
VERSION     DAOSUG010000249.1
DBLINK      BioProject: PRJNA925477
            BioSample: SAMN32864262
            Sequence Read Archive: SRR4236673
KEYWORDS    WGS; ENV; Metagenome Assembled Genome; MAG; Third Party Data; TPA;
            TPA:assembly.
SOURCE      Micropruina sp. (wastewater metagenome)
  ORGANISM  Micropruina sp.
            Bacteria; Bacillati; Actinomycetota; Actinomycetes;
            Propionibacteriales; Nocardioidaceae; Micropruina.
REFERENCE   1  (bases 1 to 4174)
  AUTHORS   Abdulkadir,N., Saraiva,J.P., Zhang,J., Stolte,S., Gillor,O.,
            Harms,H. and Rocha,U.
  TITLE     Genome-centric analyses of 165 metagenomes show that mobile genetic
            elements are crucial for the transmission of antimicrobial
            resistance genes to pathogens in activated sludge and wastewater
  JOURNAL   Microbiol Spectr 12 (3), e0291823 (2024)
   PUBMED   38289113
REFERENCE   2  (bases 1 to 4174)
  AUTHORS   Abdulkadir,N.
  TITLE     Direct Submission
  JOURNAL   Submitted (24-MAR-2023) Environmental Microbiology, Helmholtz
            Center for Environmental Research -UFZ, Permoserstr 15, Leipzig
            04318, Germany
COMMENT     The annotation was added by the NCBI Prokaryotic Genome Annotation
            Pipeline (PGAP). Information about PGAP can be found here:
            https://www.ncbi.nlm.nih.gov/genome/annotation_prok/
            
            ##Genome-Assembly-Data-START##
            Assembly Method        :: SPAdes v. 3.11.1
            Genome Representation  :: Full
            Expected Final Version :: Yes
            Genome Coverage        :: Not Applicable
            Sequencing Technology  :: Illumina MiSeq
            ##Genome-Assembly-Data-END##
            
            ##Genome-Annotation-Data-START##
            Annotation Provider               :: NCBI
            Annotation Date                   :: 05/04/2023 04:57:10
            Annotation Pipeline               :: NCBI Prokaryotic Genome
                                                 Annotation Pipeline (PGAP)
            Annotation Method                 :: Best-placed reference protein
                                                 set; GeneMarkS-2+
            Annotation Software revision      :: 6.5
            Features Annotated                :: Gene; CDS; rRNA; tRNA; ncRNA
            Genes (total)                     :: 2,776
            CDSs (total)                      :: 2,750
            Genes (coding)                    :: 2,714
            CDSs (with protein)               :: 2,714
            Genes (RNA)                       :: 26
            tRNAs                             :: 24
            ncRNAs                            :: 2
            Pseudo Genes (total)              :: 36
            CDSs (without protein)            :: 36
            Pseudo Genes (ambiguous residues) :: 0 of 36
            Pseudo Genes (frameshifted)       :: 2 of 36
            Pseudo Genes (incomplete)         :: 32 of 36
            Pseudo Genes (internal stop)      :: 2 of 36
            ##Genome-Annotation-Data-END##
FEATURES             Location/Qualifiers
     source          1..4174
                     /organism="Micropruina sp."
                     /mol_type="genomic DNA"
                     /submitter_seqid="NODE_8990_length_4174_cov_4.632435"
                     /isolate="AMR_MDS_2195"
                     /isolation_source="Activated sludge of municipal
                     wastewater treatment plants"
                     /db_xref="taxon:2737536"
                     /environmental_sample
                     /geo_loc_name="Canada"
                     /lat_lon="49.25 N 123.1 W"
                     /collection_date="2015-06-16"
                     /metagenome_source="wastewater metagenome"
                     /note="metagenomic"
     gene            complement(<1..448)
                     /locus_tag="PLF56_08805"
     CDS             complement(<1..448)
                     /locus_tag="PLF56_08805"
                     /inference="COORDINATES: ab initio
                     prediction:GeneMarkS-2+"
                     /note="Derived by automated computational analysis using
                     gene prediction method: GeneMarkS-2+."
                     /codon_start=1
                     /transl_table=11
                     /product="hypothetical protein"
                     /protein_id="HOQ53697.1"
                     /translation="MTLNDDEFRIWVERQPRGVGQLGFRGAMLKDGPQVAKTVTMSEY
                     GDTSTREVKKRELRFRTAARRTDGNGFAFDEPTRTWACEGDEVDRLLAFLQVEVGATG
                     RYRLVDSTSPQASLIDLLSSGGVDVDALVEAIIAGGNVGELVRALSR"
     gene            complement(939..1259)
                     /locus_tag="PLF56_08810"
     CDS             complement(939..1259)
                     /locus_tag="PLF56_08810"
                     /inference="COORDINATES: ab initio
                     prediction:GeneMarkS-2+"
                     /note="Derived by automated computational analysis using
                     gene prediction method: GeneMarkS-2+."
                     /codon_start=1
                     /transl_table=11
                     /product="hypothetical protein"
                     /protein_id="HOQ53698.1"
                     /translation="MYNAGRRYKTERDRRAACLQSLVNDAAEHGHGVLVLEQDDSLLS
                     WDTQHLIEFTRAAGCRDSLRYEHRRAASEHLLAIPDAIAWCWAKGGDWRRRIEPAVAD
                     VQQV"
     gene            complement(1568..2059)
                     /locus_tag="PLF56_08815"
     CDS             complement(1568..2059)
                     /locus_tag="PLF56_08815"
                     /inference="COORDINATES: ab initio
                     prediction:GeneMarkS-2+"
                     /note="Derived by automated computational analysis using
                     gene prediction method: GeneMarkS-2+."
                     /codon_start=1
                     /transl_table=11
                     /product="hypothetical protein"
                     /protein_id="HOQ53699.1"
                     /translation="MAWLEGVRQEMVDLEGFETFVKGVSTRSRKPTVTLQRRGVMSFN
                     PAAYEALGSPEGVELLFNAARKMIAVRPVKPEEVSHAVLFRGVGGKSASKNGAMLLAA
                     ISFCQTFEIDTSESVRRDVEVTDGVLFVDLNDPGVVIRGNRSAKKDGIDDAGEPQDDG
                     SDH"
     gene            complement(2239..3297)
                     /locus_tag="PLF56_08820"
     CDS             complement(2239..3297)
                     /locus_tag="PLF56_08820"
                     /inference="COORDINATES: ab initio
                     prediction:GeneMarkS-2+"
                     /note="Derived by automated computational analysis using
                     gene prediction method: GeneMarkS-2+."
                     /codon_start=1
                     /transl_table=11
                     /product="hypothetical protein"
                     /protein_id="HOQ53700.1"
                     /translation="MPTEPGQMSALSSSTARSYAMIWELFADWCDVAARRPLPADPAT
                     VLAFLADCPAAPATQRRRVIAIDHHHAAAGHPAPGDDPRVREAVGRPPIEPAPIVSEI
                     RSRVDAALRVLPSRGWTGGLFGQRDRCLLVLSQLARIPYQQLARLDAGDITITGGVAT
                     IAVPGRTWTVEAVDDPVLCGPCAAARWLTTHRIIVTKIATRAVADHLDAVESVTGDSA
                     HVCLKPMPVPRREADSPLLASPNQWGQTPFPLHPLSRLAVSQQARDLLDGIITVHREL
                     PVNPVEPAAAETVQPVAASGPGYGPEQHRAALGRRRADIAELAGVATELDEVDRRAVE
                     LNRRITELLDMAIRRDQE"
     gene            complement(3302..>4174)
                     /locus_tag="PLF56_08825"
     CDS             complement(3302..>4174)
                     /locus_tag="PLF56_08825"
                     /inference="COORDINATES: ab initio
                     prediction:GeneMarkS-2+"
                     /note="Derived by automated computational analysis using
                     gene prediction method: GeneMarkS-2+."
                     /codon_start=1
                     /transl_table=11
                     /product="hypothetical protein"
                     /protein_id="HOQ53701.1"
                     /translation="DPPGRSELVRRALSGIRRIRATPPSRRAPLLLTDIRGLVDWLAL
                     VAGGWPAGVAARRDACMLLMGFAGAFRRSELVGLSLADVTLHRTDGLHIRLRTSKTDQ
                     EAHGRTVAVPYGRDPATCAPCAWVRWRELLHAADTAPPGEQRRPVMRVLRRQAATDTP
                     VDGGEADVGDDPGSRLHVCRITRLPEPADQSRPLLRTVHRTGRIGQGPVTGHAIREII
                     RRRADEAGYTPAQIQRLGGHSLRAGFVTEAFRQGADAHAIMRQTGHRSHAVLETYARE
                     HAPLVGNAVTKLGL"
BASE COUNT          634 a         1452 c         1428 g          660 t
ORIGIN      
        1 accgcgagag ggcccgcacc agctcgccca cgttgccgcc agcgataatc gcttcgacca
       61 gcgcgtcgac gtcgacgccg ccggaggaca gcagatcgat gagcgacgcc tgcgggctcg
      121 tgctgtcgac aaggcgatag cggccggtcg cgcccacctc gacctgtagg aacgccagga
      181 ggcggtccac ctcgtcgcct tcgcacgccc acgtcctggt tggctcgtcg aaggcaaatc
      241 cgttgccgtc agttcgacgc gccgcagtgc ggaagcgcag ctcgcgcttc ttgacctcgc
      301 gggtgctcgt atctccgtac tcagacatgg tcacggtctt cgccacctgc ggcccgtcct
      361 tgagcatcgc gccgcggaag ccgagttgcc cgacccctcg aggttgccgc tccacccaga
      421 tgcggaactc atcgtcgttc agtgtcacgt ctcctcgatc tcaaaccttc acagcggaag
      481 tcggaatgtg atggggcgcc ccccgcagga aggtgatgta gccaccctcg cccacgtcca
      541 cctagtgccc gggtcccttc ggctggtttc gggcgatgtc tgagcggcct gcgggaggag
      601 ttcggccgcc gaacaggatt ccaacgcctt ccgcgcgtct gacgttgtta ctcgaccgac
      661 tgcacaccac agccgatcgc agcccgctcc tgccggccat ccacgcgggc cgctctgggt
      721 cacgccgaca atccggggcg gacccagcac ggtgagacac gatcgccggt tcaccgctgc
      781 tgcgctagtc atgccgacgg cgccgtccca gcgagggatg gcccgaggtg cccaaagatg
      841 gcacaatgag aagtgcccgt tgcgctgagc ttcgggaagt gagcccgaga cccggccgga
      901 cgaccgtggc gctcgggctt cgcgcatttt ccaccccgtc agacctgctg gacgtcggcg
      961 acggcgggct cgatgcgtcg ccgccagtcg ccgccctttg cccagcacca ggcgatcgcg
     1021 tcgggaatgg cgagaaggtg ttcgctggcc gcgcgccggt gctcgtaccg cagcgagtct
     1081 cggcagccgg cggctcgagt gaactcgatg aggtgctgag tgtcccagct gagtagcgag
     1141 tcgtcctgct cgaggacgag gacgccgtga ccgtgttcgg cagcgtcatt gacgagggac
     1201 tgaaggcagg cagctcggcg atcgcgttcg gtcttgtacc tccgaccggc gttgtacacc
     1261 gtcgcccgaa gtggactgga cgtaatggcc gacgcgatgg accggcgacg agggtcgctc
     1321 tccgagttca tgtgtagtcg gcgctgaccg ggaaggacca gttcgcgaag gactgctcga
     1381 acggcatcca ggtcgcctgc gatcacgacg ccggcgacaa ggagatagtc gcgccgcttc
     1441 gtctcgtcga cgaagacatg agcgccgctc gctgggcgag caatcacacg agaccgggtc
     1501 acgactcctg ctaccgcctc atccttccgt gtgagcgaac gggccggccg ctaggcgcga
     1561 accgacatca gtgatcgctt ccatcatcct ggggttcgcc ggcatcgtcg atgccatcct
     1621 tcttggcgct gcggttgccg cggatcacca ctccggggtc gttgagatcg acaaacagca
     1681 caccgtcggt gacctctacg tcgcgcctca ccgactccga ggtgtcaatc tcaaacgtct
     1741 gacagaacga gatcgccgcc agaagcatcg caccgttctt cgatgcactc ttgcctccaa
     1801 caccacggaa tagcacggcg tgactgacct cttccggctt gactggacgc accgctatca
     1861 tcttccgcgc tgcattgaag agcagttcaa caccctctgg gcttcctagt gcttcatatg
     1921 cggctgggtt gaaggacatg acacctcgcc gctggagcgt gaccgtgggc ttgcgagacc
     1981 gtgtggaaac gcctttgacg aaggtttcaa atccttctag gtcgaccatc tcttgccgca
     2041 ccccttctag ccaggccaca cccgcgtgag tacaagtgac tgctgtaaag gttaccagcc
     2101 agtccgccaa tcgcgtctat cgtccgacct gcgccgctgc tgacttagat cggggcgtgg
     2161 actcaacgac tcgccgcctc gtgattctct ccatcacgag cttcggtcga tggatctgcc
     2221 gcacccggaa cccatcagtc actcttgatc cctgcggatc gccatgtcca acagctcggt
     2281 gatccgccgg ttcagctcga cggctcgccg gtcgacctcg tcgagttcgg tggccacccc
     2341 ggctagctcg gcaatgtcag cccgccggcg gcccagcgcg gctcggtgct gctcgggacc
     2401 atagcccggg ccggaagctg caaccggctg caccgtctcg gcggccgccg gctctaccgg
     2461 gttcacgggg agctcgcggt ggacggtgat gattccgtcg agcaggtccc gcgcctgttg
     2521 ggatacggcc agccgggaca gcgggtggag cgggaacggt gtctgtcccc actgattggg
     2581 cgaggccagc aacggactgt ccgcctcccg ccggggcact ggcatcggct tgaggcaaac
     2641 atgggccgag tcgccggtga ctgattcgac ggcgtccagg tggtcggcga ccgcccgggt
     2701 ggcgatcttg gtgacgatga tccggtgggt ggtcaaccag cgagccgcag cgcagggccc
     2761 gcacagcacg ggatcgtcca cggcctcgac ggtccatgtc cggccgggga cggcgatggt
     2821 ggcgacgccg ccggtgatgg tgatgtcgcc ggcgtccagc cgggccagct gctggtaggg
     2881 gatgcgggcg agttgggaca gcacgagcag gcaccggtcg cgttggccga acaggccgcc
     2941 ggtccagcca cggctgggca agacccgcag cgccgcgtcg acccgggacc ggatctccga
     3001 cacgatcggc gccggttcga tgggtggccg cccgaccgcc tcccggaccc gcgggtcgtc
     3061 gcccggcgcc gggtgcccgg cggcagcgtg gtggtggtcg atggcgatga cccgccgccg
     3121 ctgcgtggcc ggggcggccg ggcagtcggc caggaacgcc aacacggtcg ccggatcggc
     3181 gggcagtggc cggcgcgcgg cgacgtcgca ccagtcggcg aacagctccc agatcatggc
     3241 gtagctgcgc gccgtcgacg acgacagcgc cgacatctga cccggttcgg tgggcatcgc
     3301 cctacaaccc cagcttggtg acggcgttgc cgaccagcgg ggcgtgctcc cgggcgtagg
     3361 tctccaggac agcgtgggag cggtgcccgg tctgccgcat gatcgcgtgc gcgtcggcgc
     3421 cctgccggaa cgcctcggtg acgaacccgg cccgcagcga gtgcccgccc aggcgttgaa
     3481 tctgcgccgg ggtgtagccg gcctcgtcgg cgcggcggcg gatgatctcc cggatcgcgt
     3541 gcccggtgac cgggccctga ccgatgcggc cggtgcggtg cactgtccgc agcagcggcc
     3601 gggactggtc ggcgggttcc ggcagccggg tgatgcggca gacgtgcaac cggctccccg
     3661 gatcgtcgcc gacgtcggcc tctcccccgt cgacgggggt gtccgtcgcg gcctgccgac
     3721 gcagcacccg catcaccggc cgccgctgtt ccccgggtgg ggcggtgtcg gcggcgtgca
     3781 gcagctcccg ccaccgcacc caggcgcacg gcgcgcaggt ggccgggtcc cgcccgtacg
     3841 gcaccgccac tgtccggccg tgcgcctcct ggtcggtctt ggacgtgcgg agccggatgt
     3901 gcaggccgtc ggtgcggtgc agagtcacgt cggccagcga cagtccgacc agttcggagc
     3961 ggcggaacgc gccggcgaac cccatcagca gcatgcaggc gtcgcggcgg gcggccaccc
     4021 cggccggcca cccgccggcg accagggcca gccagtcgac caggccgcgg atgtcggtca
     4081 gcagcaacgg cgcccgccgc gacggcgggg tggcccggat ccggcggatc ccggacagcg
     4141 cccgccggac cagttccgac cggcccggcg ggtc
//
LOCUS       DAOSUG010000250         4174 bp    DNA     linear   ENV 10-JUN-2024
DEFINITION  MAG TPA_asm: Micropruina sp. isolate AMR_MDS_2195
            NODE_8991_length_4174_cov_3.101724, whole genome shotgun sequence.
ACCESSION   DAOSUG010000250 DAOSUG010000000
VERSION     DAOSUG010000250.1
DBLINK      BioProject: PRJNA925477
            BioSample: SAMN32864262
            Sequence Read Archive: SRR4236673
KEYWORDS    WGS; ENV; Metagenome Assembled Genome; MAG; Third Party Data; TPA;
            TPA:assembly.
SOURCE      Micropruina sp. (wastewater metagenome)
  ORGANISM  Micropruina sp.
            Bacteria; Bacillati; Actinomycetota; Actinomycetes;
            Propionibacteriales; Nocardioidaceae; Micropruina.
REFERENCE   1  (bases 1 to 4174)
  AUTHORS   Abdulkadir,N., Saraiva,J.P., Zhang,J., Stolte,S., Gillor,O.,
            Harms,H. and Rocha,U.
  TITLE     Genome-centric analyses of 165 metagenomes show that mobile genetic
            elements are crucial for the transmission of antimicrobial
            resistance genes to pathogens in activated sludge and wastewater
  JOURNAL   Microbiol Spectr 12 (3), e0291823 (2024)
   PUBMED   38289113
REFERENCE   2  (bases 1 to 4174)
  AUTHORS   Abdulkadir,N.
  TITLE     Direct Submission
  JOURNAL   Submitted (24-MAR-2023) Environmental Microbiology, Helmholtz
            Center for Environmental Research -UFZ, Permoserstr 15, Leipzig
            04318, Germany
COMMENT     The annotation was added by the NCBI Prokaryotic Genome Annotation
            Pipeline (PGAP). Information about PGAP can be found here:
            https://www.ncbi.nlm.nih.gov/genome/annotation_prok/
            
            ##Genome-Assembly-Data-START##
            Assembly Method        :: SPAdes v. 3.11.1
            Genome Representation  :: Full
            Expected Final Version :: Yes
            Genome Coverage        :: Not Applicable
            Sequencing Technology  :: Illumina MiSeq
            ##Genome-Assembly-Data-END##
            
            ##Genome-Annotation-Data-START##
            Annotation Provider               :: NCBI
            Annotation Date                   :: 05/04/2023 04:57:10
            Annotation Pipeline               :: NCBI Prokaryotic Genome
                                                 Annotation Pipeline (PGAP)
            Annotation Method                 :: Best-placed reference protein
                                                 set; GeneMarkS-2+
            Annotation Software revision      :: 6.5
            Features Annotated                :: Gene; CDS; rRNA; tRNA; ncRNA
            Genes (total)                     :: 2,776
            CDSs (total)                      :: 2,750
            Genes (coding)                    :: 2,714
            CDSs (with protein)               :: 2,714
            Genes (RNA)                       :: 26
            tRNAs                             :: 24
            ncRNAs                            :: 2
            Pseudo Genes (total)              :: 36
            CDSs (without protein)            :: 36
            Pseudo Genes (ambiguous residues) :: 0 of 36
            Pseudo Genes (frameshifted)       :: 2 of 36
            Pseudo Genes (incomplete)         :: 32 of 36
            Pseudo Genes (internal stop)      :: 2 of 36
            ##Genome-Annotation-Data-END##
FEATURES             Location/Qualifiers
     source          1..4174
                     /organism="Micropruina sp."
                     /mol_type="genomic DNA"
                     /submitter_seqid="NODE_8991_length_4174_cov_3.101724"
                     /isolate="AMR_MDS_2195"
                     /isolation_source="Activated sludge of municipal
                     wastewater treatment plants"
                     /db_xref="taxon:2737536"
                     /environmental_sample
                     /geo_loc_name="Canada"
                     /lat_lon="49.25 N 123.1 W"
                     /collection_date="2015-06-16"
                     /metagenome_source="wastewater metagenome"
                     /note="metagenomic"
     gene            complement(<1..233)
                     /locus_tag="PLF56_08830"
     CDS             complement(<1..233)
                     /locus_tag="PLF56_08830"
                     /inference="COORDINATES: ab initio
                     prediction:GeneMarkS-2+"
                     /note="Derived by automated computational analysis using
                     gene prediction method: GeneMarkS-2+."
                     /codon_start=1
                     /transl_table=11
                     /product="hypothetical protein"
                     /protein_id="HOQ53702.1"
                     /translation="MEVTAAPGVSPFAGAAPLLPAPTDVAAPPAGAAVALEVSGCGPG
                     PAGPLEEPAAGDAPTPAGTPPGRWLGDGVAWLGA"
     gene            545..2068
                     /locus_tag="PLF56_08835"
     CDS             545..2068
                     /locus_tag="PLF56_08835"
                     /inference="COORDINATES: ab initio
                     prediction:GeneMarkS-2+"
                     /note="Derived by automated computational analysis using
                     gene prediction method: GeneMarkS-2+."
                     /codon_start=1
                     /transl_table=11
                     /product="hypothetical protein"
                     /protein_id="HOQ53703.1"
                     /translation="MARSSSGGGPRQFVYPPRPTAGIVGRFGIVDLAFFAAALALLWI
                     GFRLIPVSVWWLVGLGAVALLLAAFPFPRIGGRPLTAYLHPVAAAVGDRLTRRGVFRG
                     AVFAPASLAHRMDLPGDLVNFRVVAVPTPDGRSTVGLMVNDREKVACAALLTFGSAML
                     LEGSAEQGMRLDDWEAVMETFCAQGAGVSRWQLLARTAPDVVNVASRHAREHATVRTG
                     PAWQATQELIAQAAPAAQRHEVYLIVAFDLRALTDEIASLSEGSSRDEAIGVVVLERL
                     LDLERGITEARIASRGWLRPGQYAALIHTQFDPESLPLHDVLAGQDHDLDPRLAGPSA
                     TERAWKSFRHDSAVSRTVWVHELPKRPVATNWLAPVLQQSGLRRTLSLVAEPLGEVKA
                     QASAERQALRAEGSAAQRHKLGRPTTARLRKEAQAALDQDEAQADGDGVFRYHLFLTV
                     TAPDEKTLTRQMLSARRRLIRARCQSIVLYGEQDQAFFAGALPLARGLSPMRGVAGV"
     gene            2065..3738
                     /locus_tag="PLF56_08840"
     CDS             2065..3738
                     /locus_tag="PLF56_08840"
                     /inference="COORDINATES: ab initio
                     prediction:GeneMarkS-2+"
                     /note="Derived by automated computational analysis using
                     gene prediction method: GeneMarkS-2+."
                     /codon_start=1
                     /transl_table=11
                     /product="hypothetical protein"
                     /protein_id="HOQ53704.1"
                     /translation="MTTQRTSTGPTTERTSTGYAAGAGRPARRTRGAAFGALSSAVPG
                     VSVPGEVARRLAQRDAAAAAAAVEQRAERERRHLQRLTHGTGLDRRGAKGLGAGRLWG
                     RMRLQPHTAPSRMLKGIYPWIMDPGLGVPGAYVGQDLFSQASFLFDPFELYAAGVISS
                     PNMALVGEIGAGKSALMKSLILRLLVFGISFSWVQVKPEYEDLCAALGISPLRIGPGQ
                     LVRLNPLAEVRRHPDQNDIEHRAGNRSRRIALLEGLLQVALRRGMRPVERSVIGWALD
                     AATGADHNAATTLAPVSLPSLLGELVVPQRWEHRAVELGLDAADLADQAQDVRLALAE
                     LVSGSLSGMFDSTEPRNTAFDFRAVGTVVDLNRVRHNEMLTVLSMVCAQSSMEAELMH
                     PDAPRRLVGYDEAWMAMRYLPLLRRFQEQWKLSRLYGIANWIAFHRFTDLDAIGDAGS
                     ETRNLATGLLEDTGIKIAYRQGEAALPVTGALMSLSDVQEDLLRFLKQGVGLWKIGER
                     TAVVKHILSSVERPLVNTDSRMKVIAGIDDISDAEWDALLEQHATGDAA"
     gene            3735..>4174
                     /locus_tag="PLF56_08845"
     CDS             3735..>4174
                     /locus_tag="PLF56_08845"
                     /inference="COORDINATES: ab initio
                     prediction:GeneMarkS-2+"
                     /note="Derived by automated computational analysis using
                     gene prediction method: GeneMarkS-2+."
                     /codon_start=1
                     /transl_table=11
                     /product="hypothetical protein"
                     /protein_id="HOQ53705.1"
                     /translation="MIHPRSRSLRPGITGEAHAGQPARADSRSVTAGASTGSWVLLAG
                     GTVLAVAMLTAPPLAGHLAYLVTGRGWPDAGSPAAAAVGMLVSPGDPTRGWPDVQGRP
                     AVALVWALTAVLLTALVAVSAAAVLLRRRTHRPRPAGVATAAQE"
BASE COUNT          565 a         1479 c         1570 g          560 t
ORIGIN      
        1 gcgccgagcc aggccacgcc atcacccagc cagcggccag gaggcgtccc ggccggcgtc
       61 ggcgcatcac cggccgccgg ctcctcgagc ggaccggccg gtcccgggcc gcagccggag
      121 acctcgagtg caacggcggc accagcaggg ggagcagcga cgtcggtcgg tgccggcagc
      181 agcggcgccg ccccggcgaa cggcgacaca cccggcgcgg ccgtcacctc caccgccgtc
      241 agcggtgccg ccgcagcggc ggcgccgcag ccgcccccac cggcagcgtc accggcacca
      301 gcggccgcac cggtaccggc accatcggcc gccgcctcga caccgcaggc gagattcccg
      361 gccgcgccga gtcttccgcc gccggaggcc gggccaccga gagctgagcc gccacgaccc
      421 ccggaaagag ggcggacttc ttcgacaccg ccgccccggc cccggccggc ctgaccgaac
      481 cagtgaaccg agccggtgac cgatcccggc gtgaccgaac caccagagca gaaaggcgca
      541 agagatggcc aggagcagca gcggcggtgg gccccgtcag ttcgtgtacc cgccgcgacc
      601 gacggccggc atcgtgggcc ggttcggcat cgtcgacctg gccttcttcg ccgcggcgct
      661 ggccctgcta tggatcgggt tccggctgat cccggtgtcg gtgtggtggc tggtcggact
      721 cggcgcggtg gcgctgctgc tggccgcgtt cccgttcccg cggatcggcg gccgcccgct
      781 gacggcgtac ctgcatccgg tggccgccgc ggtgggggac cggctcaccc ggcgcggcgt
      841 gttccgaggg gccgtgttcg cccccgcctc gctggcccac cggatggatc tgcccggcga
      901 cctggtcaac ttccgcgtcg tggcggtgcc cacgccagac ggccggtcga cggtcgggtt
      961 gatggtcaac gaccgggaga aggtggcgtg cgcggcgctg ctcacgttcg gctccgcgat
     1021 gctgctggag ggcagcgccg agcagggcat gcggctggac gactgggaag cggtgatgga
     1081 aaccttctgc gcccagggcg cgggagtgtc ccggtggcag ttgctggccc gcaccgcccc
     1141 ggacgtggtg aacgtggcca gccggcacgc ccgggagcac gccacggtcc gcacggggcc
     1201 ggcgtggcag gcgacccagg aactgatcgc gcaggcggcg ccggcggcgc agcgccacga
     1261 ggtgtatctg atcgtcgcgt tcgacctgcg cgcgctgacc gacgagatcg cctcgctgtc
     1321 cgagggttcc agccgggacg aggcaatcgg tgtggtcgtg ttggagcggc tgctggacct
     1381 ggaacggggc atcaccgagg cccgcatcgc cagccgcggc tggctgcggc ccggccagta
     1441 cgcggcgctg atccacaccc agttcgaccc cgagtcgctg ccgctgcatg acgtgctcgc
     1501 cgggcaggac cacgacctgg acccccggct ggccggcccg tcggcgacag agcgggcgtg
     1561 gaaatcgttc cggcatgact cggcggtgtc ccgcacggtg tgggtgcacg agctgccgaa
     1621 gcggccggtc gccaccaact ggttggcgcc ggtgctgcag cagtccgggc tgcgccgcac
     1681 cctgtcgctg gtcgccgagc cgctgggtga ggtcaaggcg caggcgtccg cggagcggca
     1741 ggcgctgcgc gccgaaggct ccgccgcgca acggcacaag ctgggccggc cgaccacggc
     1801 ccggctgcgc aaggaggccc aggcggcgct ggaccaggac gaggcgcagg ccgacgggga
     1861 cggcgtgttc cggtaccacc tgttcctgac ggtcaccgcg ccggacgaga aaacactgac
     1921 ccgtcagatg ctctcggccc ggcgccggct catccgggcc cgctgccaat cgatcgtgct
     1981 gtacggggaa caggaccagg ccttcttcgc cggagcgctg ccgctggccc gcggcctgtc
     2041 cccgatgcga ggagtggccg gcgtatgacc acccaacgaa caagcaccgg cccaaccacc
     2101 gaacgcacga gcaccggata tgcggccggc gctggtcgcc ccgcccgccg gacgcggggc
     2161 gccgccttcg gcgcattgtc cagcgcggtg cccggggtgt cggtgcccgg agaggtcgcc
     2221 cgccgactgg cgcagcggga cgccgccgcc gcggccgccg cggtggagca gcgggccgag
     2281 cgggagcggc ggcacctgca acggctgacc cacggcaccg ggctggaccg ccgcggcgcg
     2341 aaaggcctgg gtgcgggcag gttgtggggg cggatgcggc tgcaaccgca caccgcgccg
     2401 tcccggatgc tcaagggcat ctacccgtgg atcatggatc ccgggttggg ggtgcccggc
     2461 gcctacgtcg ggcaggacct gttcagccag gccagtttcc tgttcgaccc gttcgagttg
     2521 tatgcggcgg gggtgatctc ctcgccgaac atggcgctgg tcggagagat cggcgccggc
     2581 aagtccgcgc tgatgaaatc gctgatcctg cggctgctgg tgttcggaat ctcgttctcc
     2641 tgggtgcagg tcaaacccga gtacgaggac ctgtgcgccg ccctgggcat ctcgccgctg
     2701 cggatcggcc ccgggcagct ggttcggctc aacccgttgg ccgaggtgcg tcggcacccg
     2761 gaccagaacg acatcgagca ccgggccggc aaccgatctc gtcgcatcgc cctgctggaa
     2821 gggctgctgc aggtcgcgct gcgccgcggg atgcggccgg tggaacggtc ggtgatcggg
     2881 tgggcgttgg acgccgccac cggcgccgac cacaacgccg cgacgacgct ggcgcctgtg
     2941 tcgctgccgt ccctgctcgg cgagctggtc gtgccgcaac ggtgggagca tcgcgccgtc
     3001 gagctggggt tggacgcggc ggacctagcc gatcaggcgc aggacgtgcg gctggcgctg
     3061 gccgagctgg tgtcggggtc gctgtcgggg atgttcgact cgaccgagcc gcgcaacacc
     3121 gcgttcgact tcagggcagt gggcaccgtg gtcgatctga accgggtgcg gcacaacgag
     3181 atgctcaccg tgctgtccat ggtgtgcgcg cagtcgtcga tggaagccga gctcatgcac
     3241 ccggacgcac cgcgtcggct ggtcggctac gacgaggcgt ggatggcgat gcggtacctg
     3301 ccgctgctgc gccgattcca ggagcagtgg aagctgtccc ggctctacgg catcgcgaac
     3361 tggatcgcct ttcaccggtt caccgacctg gacgccatcg gcgacgccgg cagcgagacc
     3421 cgcaacctgg ccaccggcct gctggaggac accggtatca agatcgccta ccggcagggt
     3481 gaggcggcgc tgccggtgac cggtgcgttg atgagcctgt cggacgtgca ggaggacctg
     3541 ctgcggttcc ttaagcaggg cgtgggcctg tggaagatcg gcgagcggac cgcggtggtc
     3601 aagcacatct tgtcctcggt ggagaggccg ctggtcaaca ccgactcccg gatgaaggtc
     3661 atcgccggca tcgatgacat ctccgacgcc gaatgggacg ccctgctcga gcagcacgcc
     3721 accggagacg cggcgtgatc caccctcgct cccgttccct acgacctggg atcaccggcg
     3781 aggcgcacgc ggggcagccg gcgcgtgcgg acagccggtc ggtcaccgcc ggcgcctcca
     3841 ccggcagttg ggtgctgctg gcaggcggca ccgtcctggc cgtggcgatg cttacggcgc
     3901 cgccgctggc cggtcatctg gcctatctgg tcaccggccg tggatggccg gacgccggat
     3961 ccccggcggc ggccgccgtg ggcatgctgg tctcgcccgg tgacccgacc cggggctggc
     4021 ccgacgtcca gggccggccg gcggtggcgc tggtgtgggc gctgaccgcg gtcctgctca
     4081 ccgcgctggt ggccgtgagc gccgcggctg tgctgctgcg gcggcggacg caccggcccc
     4141 gccccgcggg ggtggccacc gcggcccagg aaag
//
LOCUS       DAOSUG010000251         4173 bp    DNA     linear   ENV 10-JUN-2024
DEFINITION  MAG TPA_asm: Micropruina sp. isolate AMR_MDS_2195
            NODE_8993_length_4173_cov_4.135988, whole genome shotgun sequence.
ACCESSION   DAOSUG010000251 DAOSUG010000000
VERSION     DAOSUG010000251.1
DBLINK      BioProject: PRJNA925477
            BioSample: SAMN32864262
            Sequence Read Archive: SRR4236673
KEYWORDS    WGS; ENV; Metagenome Assembled Genome; MAG; Third Party Data; TPA;
            TPA:assembly.
SOURCE      Micropruina sp. (wastewater metagenome)
  ORGANISM  Micropruina sp.
            Bacteria; Bacillati; Actinomycetota; Actinomycetes;
            Propionibacteriales; Nocardioidaceae; Micropruina.
REFERENCE   1  (bases 1 to 4173)
  AUTHORS   Abdulkadir,N., Saraiva,J.P., Zhang,J., Stolte,S., Gillor,O.,
            Harms,H. and Rocha,U.
  TITLE     Genome-centric analyses of 165 metagenomes show that mobile genetic
            elements are crucial for the transmission of antimicrobial
            resistance genes to pathogens in activated sludge and wastewater
  JOURNAL   Microbiol Spectr 12 (3), e0291823 (2024)
   PUBMED   38289113
REFERENCE   2  (bases 1 to 4173)
  AUTHORS   Abdulkadir,N.
  TITLE     Direct Submission
  JOURNAL   Submitted (24-MAR-2023) Environmental Microbiology, Helmholtz
            Center for Environmental Research -UFZ, Permoserstr 15, Leipzig
            04318, Germany
COMMENT     The annotation was added by the NCBI Prokaryotic Genome Annotation
            Pipeline (PGAP). Information about PGAP can be found here:
            https://www.ncbi.nlm.nih.gov/genome/annotation_prok/
            
            ##Genome-Assembly-Data-START##
            Assembly Method        :: SPAdes v. 3.11.1
            Genome Representation  :: Full
            Expected Final Version :: Yes
            Genome Coverage        :: Not Applicable
            Sequencing Technology  :: Illumina MiSeq
            ##Genome-Assembly-Data-END##
            
            ##Genome-Annotation-Data-START##
            Annotation Provider               :: NCBI
            Annotation Date                   :: 05/04/2023 04:57:10
            Annotation Pipeline               :: NCBI Prokaryotic Genome
                                                 Annotation Pipeline (PGAP)
            Annotation Method                 :: Best-placed reference protein
                                                 set; GeneMarkS-2+
            Annotation Software revision      :: 6.5
            Features Annotated                :: Gene; CDS; rRNA; tRNA; ncRNA
            Genes (total)                     :: 2,776
            CDSs (total)                      :: 2,750
            Genes (coding)                    :: 2,714
            CDSs (with protein)               :: 2,714
            Genes (RNA)                       :: 26
            tRNAs                             :: 24
            ncRNAs                            :: 2
            Pseudo Genes (total)              :: 36
            CDSs (without protein)            :: 36
            Pseudo Genes (ambiguous residues) :: 0 of 36
            Pseudo Genes (frameshifted)       :: 2 of 36
            Pseudo Genes (incomplete)         :: 32 of 36
            Pseudo Genes (internal stop)      :: 2 of 36
            ##Genome-Annotation-Data-END##
FEATURES             Location/Qualifiers
     source          1..4173
                     /organism="Micropruina sp."
                     /mol_type="genomic DNA"
                     /submitter_seqid="NODE_8993_length_4173_cov_4.135988"
                     /isolate="AMR_MDS_2195"
                     /isolation_source="Activated sludge of municipal
                     wastewater treatment plants"
                     /db_xref="taxon:2737536"
                     /environmental_sample
                     /geo_loc_name="Canada"
                     /lat_lon="49.25 N 123.1 W"
                     /collection_date="2015-06-16"
                     /metagenome_source="wastewater metagenome"
                     /note="metagenomic"
     gene            <1..271
                     /locus_tag="PLF56_08850"
     CDS             <1..271
                     /locus_tag="PLF56_08850"
                     /inference="COORDINATES: protein motif:HMM:NF014634.2"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_function: GO:0008887 - glycerate kinase activity
                     [Evidence IEA];
                     GO_process: GO:0031388 - organic acid phosphorylation
                     [Evidence IEA]"
                     /codon_start=2
                     /transl_table=11
                     /product="glycerate kinase"
                     /protein_id="HOQ53706.1"
                     /translation="LVLTGEGSMDAQTLSGKTPAGVAEVAARFGVPVIAFAGRLGDDA
                     EVLVGNGFAAVVPIATGPCDLASALADGALNLQRAVATAMRLYQL"
     gene            682..1341
                     /locus_tag="PLF56_08855"
     CDS             682..1341
                     /locus_tag="PLF56_08855"
                     /inference="COORDINATES: protein motif:HMM:NF024771.2"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="mandelate racemase/muconate lactonizing enzyme
                     family protein"
                     /protein_id="HOQ53707.1"
                     /translation="MLENATASREAGIGGIKIKVGHPDRNVDMTRLTAMREHLGDDFP
                     LMVDANQQWDRPTAMRMCRAMEPFNLIWIEEPLDAYDYLGHAELARAIDTPIATGEML
                     ASIAEHVKLIENGSCDIIQPDAPRVGGITEFLRLTTLAAQAGLQLAPHFAMEIHCHLA
                     ATYPIEPWVEHFDWLDPLFNERMQIADGRMIVSDRPGLGVTLSDQLRAWTVDSCVIKA
                     G"
     gene            1605..3281
                     /locus_tag="PLF56_08860"
     CDS             1605..3281
                     /locus_tag="PLF56_08860"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_013864473.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_component: GO:0055052 - ATP-binding cassette (ABC)
                     transporter complex, substrate-binding subunit-containing
                     [Evidence IEA];
                     GO_function: GO:0042626 - ATPase-coupled transmembrane
                     transporter activity [Evidence IEA];
                     GO_function: GO:0140359 - ABC-type transporter activity
                     [Evidence IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="ABC transporter substrate-binding protein"
                     /protein_id="HOQ53708.1"
                     /translation="MRKQISLRRGVAATAAVALLAGFGGCVQSQRATTSPGATTGGDT
                     KDTFVFAASSDPVMLDPAMASDGETFRVARQQFEGLLGTKPGTTELEPLLATAWKASD
                     DNSSFTFTLRDGVKFSDGTDFNAEAVCANFDRWYNWTGINQSENITYYYGKLMRGFKT
                     GANSGDALYKNCEAKSPTEVTINLTSPFVHFPDAMTLPAFSMQSPTALKKYNADNTSG
                     SEDDPRFSEYATAHPTGTGPFVFESWDRGQQVKLKRNDNYWGDKAKVSEVIIRTISDP
                     KARTQELQAGNIDGYDLVAPADQSTLKNAGFQILNRPAFNILYLGMNQKVKALSDIRV
                     RQAIAYAIDKDALIKQVMPEGTTAAINFMPDNVTGWNGNVEKYAYNPDKAKQLLKEAG
                     QENLTLDFNYPTGVSRPYMPSPEDIYTSLKAQLEAVGITVKPVANKWSPDYLDLIQGG
                     SSHGIHLLGWTGDYNDPDNFVGVFFGTKANEWGFDNPELFKALSEARAMGDAAAQKAK
                     YEEINKMIADFVPGVPLTHAPPSLAFAKGVQGYVPSPVQDEVWNVVTITK"
     gene            3368..>4173
                     /locus_tag="PLF56_08865"
     CDS             3368..>4173
                     /locus_tag="PLF56_08865"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_013864472.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_process: GO:0055085 - transmembrane transport [Evidence
                     IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="ABC transporter permease"
                     /protein_id="HOQ53709.1"
                     /translation="MLRFAVRRILLLIPVLFGLSVLLFLWLRALPGDPARALLGEKAT
                     PEAIARINEQYGFNKPILQQYLTYVGQLARGDFGSSPRTGEPVVETFLLRFPATIELA
                     VAALLFAVVVGIPLGYLAAKRAGGLIDTLTVSASLAGVVIPVFVLAYLLKVVFAVGLP
                     GWEWLAVLPSSGRQTVTIDATHVTNFYVLDGLLTGEFDAAWDAILHLILPAIALGSIP
                     LAIIVRMTRASVLDVLHEDYVRTAKAKGLDPGLISRRHVLRNALLPVTTT"
BASE COUNT          762 a         1439 c         1306 g          666 t
ORIGIN      
        1 cctggtgctc accggggagg gctcgatgga cgcccagacc ctgtcgggca agacccccgc
       61 cggggtcgcc gaggtcgccg cacgcttcgg cgtgcccgtg atcgccttcg ccggacgcct
      121 cggtgacgac gcggaggtgc tggtcggcaa cggtttcgcg gcggtggtgc cgatcgccac
      181 gggtccgtgc gacctggcct ccgctctcgc ggacggtgcg ctgaacctgc aacgcgccgt
      241 ggcgaccgcg atgcggctct accaactctg agccggcatc ccggcgcata gcgtcatgcc
      301 ggcggatccg tcatcccggc gaacgccggg atctcggttg gcgcgctgcg gacgttgcgg
      361 gtggaccgcg agacgcagct atgaggcgcc gcagcggctg acgcaacgtg aggacctgag
      421 cagagatccc ggctttcgcc gggatgacag accaagaaca acaaggagaa acacatgtcc
      481 gacagcattc gcagcatcgc gctcagttcc gtggtgctgc ctttgaagac cgagatcagc
      541 gacgccaaag tgttcaccgg cggcagaagc cgatgactca ggttgacttc ctgttcgccg
      601 agatcgcgac cgcgcacggc catagcgggg tgggcttctc ataccacctc aggcggattt
      661 ctcaacaccc cgctggaaca gatgctcgag aacgccaccg ccagccgtga ggcgggcatc
      721 ggcggtatca agatcaaggt cggtcacccg gatcgcaacg tcgacatgac ccggctcacc
      781 gccatgcgtg agcacctggg tgacgacttt ccgttgatgg tggacgcaaa ccagcagtgg
      841 gaccgtccga ccgccatgcg tatgtgccga gccatggagc cgttcaacct gatctggatc
      901 gaagagccgc tggacgccta cgactacctc ggtcacgccg aactggcccg cgccatcgac
      961 accccgatcg ccaccggtga aatgctcgcc tcgatcgccg agcacgtgaa gctgatcgag
     1021 aacggctcct gcgacatcat tcagcccgac gccccgcgcg taggcggcat caccgagttc
     1081 ctgcggctca ccactctggc cgcccaggcc gggctgcagt tggcgccgca cttcgccatg
     1141 gagatccact gtcacctggc tgccacctac ccgatcgaac cttgggtcga gcacttcgac
     1201 tggctcgacc cgctgttcaa cgaacgcatg cagatcgcgg acggacgcat gatcgtgtcc
     1261 gatcgccctg gactgggagt gaccctgtcc gaccagcttc gggcctggac ggtggacagt
     1321 tgcgtgatca aggcaggctg agggcgagca agatcagctg cgcaccggag tccaaccccg
     1381 cctaactttc cagcgcctgc gcaactccgc ggagttgcgc aggcgctgga aagttagggg
     1441 ggcgctcgcg ggctcgtcgg cacccgaccc gacacccgga ggtggcagtt acaacacgaa
     1501 tgcataacag ctcggcaatt cggtggccgg tgagtagcca ccccggtagc ctccccagac
     1561 aagctacggc ggcatcaagc cgtgcatcct gatgggagaa caagttgaga aagcagattt
     1621 ccctgcgccg cggggtcgcc gcgaccgctg cggttgcgct gctggccggc ttcggcggct
     1681 gcgtgcagag ccagcgtgcg accaccagcc ccggtgcgac caccggtggc gacaccaagg
     1741 acacgttcgt gttcgcggca tcgtccgacc cggtgatgct cgacccggcg atggccagtg
     1801 acggcgagac cttccgggtt gcccgtcagc agttcgaggg cctgctcggc accaagcccg
     1861 gcaccaccga gctcgagccc ctgttggcca ccgcctggaa ggcctcggac gacaactcgt
     1921 cgttcacgtt cacgctgcgc gacggggtca agttctctga cggcaccgac ttcaacgccg
     1981 aggccgtctg tgccaacttc gaccgttggt acaactggac cggcatcaac cagtccgaga
     2041 acatcaccta ctactacggc aagctgatgc gcggtttcaa gaccggcgcc aacagcggtg
     2101 acgcgctgta caagaactgt gaggccaaga gccccaccga ggtgacgatc aacctgacca
     2161 gccccttcgt gcactttccg gacgccatga cgctgccggc cttctcgatg cagtcgccga
     2221 ccgcgttgaa gaagtacaac gccgacaaca ccagcggcag cgaggacgac ccccgcttct
     2281 ctgagtacgc gaccgcgcac ccgaccggca ccggcccgtt cgtgttcgag tcgtgggatc
     2341 gtggccagca ggtcaagctc aagcgcaacg acaactactg gggcgacaag gccaaggtct
     2401 ccgaggtgat cattcgcacc atctccgacc ccaaggcccg cacccaggaa ctgcaggccg
     2461 gcaacatcga cggctacgac ctggtggctc cggccgacca gtccacgctg aagaacgcgg
     2521 gcttccagat tctgaaccgt ccggcgttca acattctgta cctgggcatg aaccagaagg
     2581 tgaaggccct gtcggacatc cgggtgcgcc aggccatcgc ctacgcgatc gacaaggacg
     2641 ccctgatcaa gcaggtgatg cccgagggca ccacggcagc gatcaacttc atgcccgaca
     2701 acgtgaccgg ctggaacggc aacgtcgaga agtacgccta caaccccgac aaggccaagc
     2761 agctgttgaa ggaggccggg caagagaacc tgaccctcga cttcaactac ccgaccggcg
     2821 tctcgcggcc ctacatgcct tcgcccgaag acatctacac ctcgctgaag gcgcagctcg
     2881 aagcggtcgg catcacggtg aagccggtgg ccaacaagtg gagccccgac tacctcgacc
     2941 tgattcaggg cggcagcagc cacggcatcc acctgctggg ctggaccggt gactacaacg
     3001 atcccgacaa cttcgtcggc gtgttcttcg gcaccaaggc caacgagtgg ggcttcgaca
     3061 accccgaact gttcaaggcg ctcagcgagg cgcgcgccat gggcgatgcg gccgctcaga
     3121 aggccaagta cgaagagatc aacaagatga tcgccgactt cgtgcccggt gttccgctca
     3181 cccacgcccc gccgtccctg gccttcgcca agggcgtcca gggttacgtg ccctcgccag
     3241 tgcaggacga ggtgtggaac gtcgtgacga tcaccaagtg atcgcctcac cgatcgcttg
     3301 atctgcgggc cgggtcggta ccaccattgc gtgggcccga cccggccccc gacaccaaag
     3361 gcaccgcatg ctcaggttcg ccgtccgccg cattctgctg ctgatcccgg tgctgttcgg
     3421 gctgagcgtg ctgctgttcc tgtggcttcg ggcgctgccg ggcgacccgg cccgggcact
     3481 gctcggcgag aaggcgacgc ccgaggcgat cgccaggatc aacgagcagt acggcttcaa
     3541 caagccgatc ctgcagcagt acctcaccta cgtcggccag ttggcccgcg gcgacttcgg
     3601 cagcagcccc cgcacgggtg aaccggtggt cgagaccttt ctgctgcgct ttccggccac
     3661 catcgaactc gccgtcgcgg ccctgctgtt cgcggtcgtg gtcggcattc cgctggggta
     3721 cctggccgcc aagcgggccg ggggcctgat cgacacgctc accgtgtcgg cctcgctggc
     3781 cggtgtggtg attccggtct tcgtgctcgc ctacctgctc aaggtggtgt tcgcggtcgg
     3841 gctgcccggc tgggagtggc tcgcggtgct gccgtcgtcc ggtagacaga cggtcaccat
     3901 cgacgccacc cacgtgacga acttctacgt gctggacggc ctgctcaccg gcgagttcga
     3961 cgccgcgtgg gacgccatcc tgcacctgat tctgcccgcc atcgcgctgg gcagcattcc
     4021 gctggccatc atcgtgcgca tgacccgggc ctcggtgctc gacgtgctgc acgaggacta
     4081 cgtgcgcacc gccaaggcca aggggctcga ccccggcctg atcagccgca ggcacgtgct
     4141 gcgcaacgcg ctgctgccgg tcaccaccac cat
//
LOCUS       DAOSUG010000252         4157 bp    DNA     linear   ENV 10-JUN-2024
DEFINITION  MAG TPA_asm: Micropruina sp. isolate AMR_MDS_2195
            NODE_9020_length_4157_cov_3.814481, whole genome shotgun sequence.
ACCESSION   DAOSUG010000252 DAOSUG010000000
VERSION     DAOSUG010000252.1
DBLINK      BioProject: PRJNA925477
            BioSample: SAMN32864262
            Sequence Read Archive: SRR4236673
KEYWORDS    WGS; ENV; Metagenome Assembled Genome; MAG; Third Party Data; TPA;
            TPA:assembly.
SOURCE      Micropruina sp. (wastewater metagenome)
  ORGANISM  Micropruina sp.
            Bacteria; Bacillati; Actinomycetota; Actinomycetes;
            Propionibacteriales; Nocardioidaceae; Micropruina.
REFERENCE   1  (bases 1 to 4157)
  AUTHORS   Abdulkadir,N., Saraiva,J.P., Zhang,J., Stolte,S., Gillor,O.,
            Harms,H. and Rocha,U.
  TITLE     Genome-centric analyses of 165 metagenomes show that mobile genetic
            elements are crucial for the transmission of antimicrobial
            resistance genes to pathogens in activated sludge and wastewater
  JOURNAL   Microbiol Spectr 12 (3), e0291823 (2024)
   PUBMED   38289113
REFERENCE   2  (bases 1 to 4157)
  AUTHORS   Abdulkadir,N.
  TITLE     Direct Submission
  JOURNAL   Submitted (24-MAR-2023) Environmental Microbiology, Helmholtz
            Center for Environmental Research -UFZ, Permoserstr 15, Leipzig
            04318, Germany
COMMENT     The annotation was added by the NCBI Prokaryotic Genome Annotation
            Pipeline (PGAP). Information about PGAP can be found here:
            https://www.ncbi.nlm.nih.gov/genome/annotation_prok/
            
            ##Genome-Assembly-Data-START##
            Assembly Method        :: SPAdes v. 3.11.1
            Genome Representation  :: Full
            Expected Final Version :: Yes
            Genome Coverage        :: Not Applicable
            Sequencing Technology  :: Illumina MiSeq
            ##Genome-Assembly-Data-END##
            
            ##Genome-Annotation-Data-START##
            Annotation Provider               :: NCBI
            Annotation Date                   :: 05/04/2023 04:57:10
            Annotation Pipeline               :: NCBI Prokaryotic Genome
                                                 Annotation Pipeline (PGAP)
            Annotation Method                 :: Best-placed reference protein
                                                 set; GeneMarkS-2+
            Annotation Software revision      :: 6.5
            Features Annotated                :: Gene; CDS; rRNA; tRNA; ncRNA
            Genes (total)                     :: 2,776
            CDSs (total)                      :: 2,750
            Genes (coding)                    :: 2,714
            CDSs (with protein)               :: 2,714
            Genes (RNA)                       :: 26
            tRNAs                             :: 24
            ncRNAs                            :: 2
            Pseudo Genes (total)              :: 36
            CDSs (without protein)            :: 36
            Pseudo Genes (ambiguous residues) :: 0 of 36
            Pseudo Genes (frameshifted)       :: 2 of 36
            Pseudo Genes (incomplete)         :: 32 of 36
            Pseudo Genes (internal stop)      :: 2 of 36
            ##Genome-Annotation-Data-END##
FEATURES             Location/Qualifiers
     source          1..4157
                     /organism="Micropruina sp."
                     /mol_type="genomic DNA"
                     /submitter_seqid="NODE_9020_length_4157_cov_3.814481"
                     /isolate="AMR_MDS_2195"
                     /isolation_source="Activated sludge of municipal
                     wastewater treatment plants"
                     /db_xref="taxon:2737536"
                     /environmental_sample
                     /geo_loc_name="Canada"
                     /lat_lon="49.25 N 123.1 W"
                     /collection_date="2015-06-16"
                     /metagenome_source="wastewater metagenome"
                     /note="metagenomic"
     gene            complement(<1..1110)
                     /locus_tag="PLF56_08870"
     CDS             complement(<1..1110)
                     /locus_tag="PLF56_08870"
                     /inference="COORDINATES: protein motif:HMM:NF019305.2"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_function: GO:0030170 - pyridoxal phosphate binding
                     [Evidence IEA];
                     GO_process: GO:0009058 - biosynthetic process [Evidence
                     IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="aminotransferase class I/II-fold pyridoxal
                     phosphate-dependent enzyme"
                     /protein_id="HOQ53710.1"
                     /translation="MVEIDPLLDAALPAGLAGLYIGGGFPEVHAVELSRNAALRTAIG
                     DAVAAGLPTVAECAGQLYLADHLDGHPMVGTLPASARMTPRLSLGYRAAVAPADTLLA
                     AAGQAVSGHEFHRTTTTPSAGVAPAWTWADTPAGFSLDPAGSGRPTVHASYLHVHWAG
                     HPQCAQRFADAAARFAAAQSSDEFVAAAGPAPARPALGDGIDLHHHGDHDIAPDLVDL
                     AVNVLLPGPPDWLAATIDGTTASLGSYPDAGAARAALAAAHGVPERMVLPTAGGAEAF
                     ALVARTLTARHPLVVHPQFTEPEAALRAAGHAVQRWLLPVHAGRDVPALTGLPAWADA
                     LFVGNPTNPTGWLHTREALLGAGEGRLLVVDEAFMD"
     gene            complement(1114..1953)
                     /locus_tag="PLF56_08875"
     CDS             complement(1114..1953)
                     /locus_tag="PLF56_08875"
                     /inference="COORDINATES: protein motif:HMM:NF002204.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_function: GO:0042242 - cobyrinic acid a,c-diamide
                     synthase activity [Evidence IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="cobyrinate a,c-diamide synthase"
                     /protein_id="HOQ53711.1"
                     /translation="MVSIPRVVIAAANSSAGKTTIATGLMAALRSSGRVVAGFKVGPD
                     FIDPGYHTLATGRPGRNLDVVLTSEQLVPRLFLHGVTRPTPADVAIVEGVMGLFDGQL
                     GRDGHGSTAHVARLLDAPVVLVVDAAGSSRTAAAAALGLRHFDPRVRIAGVVVNRVAS
                     ERHAAELAAVFERAGLPVLGTVPRSAAIAAPSRHLGLVPAAERAESAATIKALATHIA
                     AHVDLDALLDLAATAGPLQATAWDPAHVVRPVADRPVIGLLAGRAFTFRYPETAELLA
                     AAG"
     gene            complement(1947..2561)
                     /gene="cobO"
                     /locus_tag="PLF56_08880"
     CDS             complement(1947..2561)
                     /gene="cobO"
                     /locus_tag="PLF56_08880"
                     /EC_number="2.5.1.17"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_017935462.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_function: GO:0008817 - corrinoid adenosyltransferase
                     activity [Evidence IEA];
                     GO_process: GO:0009236 - cobalamin biosynthetic process
                     [Evidence IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="cob(I)yrinic acid a,c-diamide
                     adenosyltransferase"
                     /protein_id="HOQ53712.1"
                     /translation="MPEGQPADVPADGISTRERRLRPALIVNTGEGKGKTTAAMGLAL
                     RAWHQGWSIGVFQFIKSGKWVSGERAALEALGAEHERTGAGGEVAWFATGWGRHWVRG
                     AGTDPDRADAAREAWAEVADRLATQRHRLYVLDELTYPLTWGWLDIDDVVRALRDRPG
                     VQHVVVTGRNAPAELLEIATTVTEMTKRKHPFDLGQKGQAGIEW"
     gene            2625..3212
                     /gene="cbiT"
                     /locus_tag="PLF56_08885"
     CDS             2625..3212
                     /gene="cbiT"
                     /locus_tag="PLF56_08885"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_015069640.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_function: GO:0046025 - precorrin-6Y
                     C5,15-methyltransferase (decarboxylating) activity
                     [Evidence IEA];
                     GO_process: GO:0046140 - corrin biosynthetic process
                     [Evidence IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="precorrin-6Y C5,15-methyltransferase
                     (decarboxylating) subunit CbiT"
                     /protein_id="HOQ53713.1"
                     /translation="MNDELGIDGRLTAGLPDEAFVTDGLLTKRVLRAFALAMLAPRAG
                     ELLWDLGAGTGSIAVEWCRLHPANRAVAVERNAERAARILINAENLGAAGRIDVRTAA
                     VADVLAELPAPDAVFVGGGVRAEILDEGWDALPSGGRLVVHSVTADSDAVLLALHQRV
                     GGELSRIAVEVAEPIGRFTGFRPLRTVTCWAATKG"
     gene            3486..>4157
                     /locus_tag="PLF56_08890"
     CDS             3486..>4157
                     /locus_tag="PLF56_08890"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_015069636.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_component: GO:0016020 - membrane [Evidence IEA];
                     GO_component: GO:0043190 - ATP-binding cassette (ABC)
                     transporter complex [Evidence IEA];
                     GO_process: GO:0000041 - transition metal ion transport
                     [Evidence IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="energy-coupling factor ABC transporter permease"
                     /protein_id="HOQ53714.1"
                     /translation="MHIAEGFLPVVHCVAWYAVSAPFVIHGARKVIEEAKEHPESRLL
                     LAAAGAFTFVLSSIKLPSVTGSSSHPTGTGLGAVLFRPPVMAFMATVVLIFQALLLAH
                     GGITTLGANVFSMGIAGPWIGFWCWQLSNKLGWHRNVGMFLALAFADLSTYVVTSVQL
                     GLAFPDPVSGVVGSVAKFMGVFAISQVPLAIAEGILGVLVFRVLIDVAKPELERLGVI
                     KPAAPA"
BASE COUNT          539 a         1476 c         1538 g          604 t
ORIGIN      
        1 gtccatgaac gcctcgtcga ccaccagcag ccgtccttca ccggccccga gcagggcctc
       61 gcgggtgtgc agccagccgg tcgggttggt tgggttgccg acgaacagcg cgtcggccca
      121 ggcgggcagg ccggtgagcg cgggcacgtc gcgtccggca tgcaccggca gcagccagcg
      181 ctgcacggcg tggcccgcgg cgcgcagggc cgcctcgggt tcggtgaact gcgggtgcac
      241 gaccagcggg tgccgggcgg tcagggtgcg ggcgaccagc gcgaacgcct cggcgccgcc
      301 ggcggtgggc agcaccatcc gctccggcac cccgtgcgcg gccgcgaggg cggcgcgggc
      361 ggcgccggcg tcgggatagc tgcccagcga cgcggtggtc ccgtcgatgg tggcggccag
      421 ccagtccggc gggccgggca gcagcacgtt gacggccagg tcgaccaggt cgggggcgat
      481 gtcgtggtcg ccgtggtggt gcaggtcgat gccgtcgccg agtgccggac gcgccggcgc
      541 cgggccggcc gcagcgacga actcgtccga ggactgtgcg gccgcgaacc gggcggcggc
      601 gtcagcgaac cgctgggcgc actgcgggtg cccggcccag tgcacgtgca ggtaggacgc
      661 gtgaacggtg ggacgtccgc tgcctgccgg gtcgagcgag aagcccgcgg gggtatccgc
      721 ccaggtccag gccggggcga cgcccgcgga tggggtggtc gtggtgcggt ggaactcgtg
      781 gccgctgacc gcctggccgg ccgcagcgag cagggtgtcg gcgggtgcca cggcggcgcg
      841 gtagccgagg ctgagccgcg gcgtcatccg ggccgaggcc ggcagcgtcc cgaccatcgg
      901 atggccgtcc aggtggtcgg ccaggtacag ctggccggcg cattcggcga ccgtcggcag
      961 cccggccgcg accgcgtccc cgatggccgt gcgcagcgcc gcgttgcggc tgagttcgac
     1021 ggcgtgcacc tcgggaaagc cgccgccgat gtacagcccg gccaggccgg cgggcagggc
     1081 cgcgtccagc agcgggtcga tttcgaccac ctgtcagcct gccgcggcga gcagttcggc
     1141 cgtttcgggg tagcgaaagg tgaaggcccg tccggcgagc aggccgatca ccggacggtc
     1201 ggcgaccgga cgcaccacgt gcgccggatc ccaggccgtc gcctgcagcg ggccggccgt
     1261 ggcggccagg tcgagcaggg cgtccaggtc gacgtgagcg gcgatgtggg tggcgagtgc
     1321 cttgatggtg gccgccgatt cggcccgctc ggcggccggt accagcccca ggtgccgcga
     1381 cggcgcggcg atcgcggcgc tgcgcggcac ggtgcccagc accggcaatc cggcgcgctc
     1441 gaacaccgcg gcgagttcgg cggcgtgtcg ttcgctcgcc acccggttca ccacgacccc
     1501 ggcgatccgc acccgcggat cgaagtgccg cagtccgagg gcggccgccg cagcggtgcg
     1561 cgacgacccg gcggcgtcca cgaccaacac gaccggggcg tcgagcagcc gggcgacgtg
     1621 cgcggtggag ccgtggccgt cgcggcccag ttggccgtcg aacaggccca tcacgccctc
     1681 gacgatggcg acgtcggccg gcgtgggccg cgtgacgccg tgcaggaaca gtcgcggcac
     1741 cagctgctcg ctggtcagca ccacgtccag gttgcgtccg ggacggcccg tcgccaatgt
     1801 gtgataaccc ggatcgatga aatccgggcc gaccttgaag ccggccacca cccgtccgga
     1861 cgaacgcagc gcggccatca gcccggtcgc gatggtggtc ttgccggccg aggagttggc
     1921 ggcggcgatg accacccgtg gaatgcttac cattcgatgc ccgcctgccc cttctggccg
     1981 agatcgaacg ggtgcttgcg cttggtcatc tcggtgacgg tggtggcgat ctcgagcaac
     2041 tcggcagggg cgttccggcc ggtcaccacc acgtgctgaa cccctggtcg atcccgcaat
     2101 gcacgcacca cgtcgtcgat gtcgagccac ccccaggtca gcggataggt cagttcgtcc
     2161 agcacgtaca gccggtgccg ctgcgtcgcg agccgatcgg cgacctcggc ccaggcctcg
     2221 cgcgcggcgt ccgctcggtc gggatcggtg cccgccccgc gcacccagtg gcggccccaa
     2281 ccggtggcga accaggccac ctcgccgccg gcgccggtgc gctcgtgctc ggcgccgagc
     2341 gcttcgagcg cggcccgctc gccgctgacc cacttgcccg acttgatgaa ttggaacacc
     2401 ccgatcgacc agccctggtg ccaggcccgc agcgccaggc ccatcgcggc ggtggtcttg
     2461 cccttgccct cgccggtgtt cacgatcagc gccggacgca gccgccgctc gcgggtgctg
     2521 attccgtcgg ccggcacgtc ggccggttgg ccctcaggca tgtcgcgctt gcgtccgggt
     2581 gctcacggcc cccgattgtg ccgcatctag ctactctgct ggccatgaac gacgaactcg
     2641 gcatcgacgg ccggctcacc gcaggcttgc ccgatgaggc tttcgtcacc gacggcctgc
     2701 tgaccaagcg ggtgctgcgc gccttcgccc tggcgatgct ggcgccgcgc gccggcgagc
     2761 tgttgtggga cctcggtgcg ggcaccggct cgatcgccgt cgagtggtgc cggctgcacc
     2821 cggccaaccg ggcggtcgcc gtcgagcgga acgccgagcg ggccgcgcgg atcctgatca
     2881 acgccgagaa cctcggtgcg gccggacgga tcgacgtccg caccgcggcg gtagccgacg
     2941 tgctggccga gctgcccgcg ccggacgcgg tcttcgtcgg cggcggcgtc cgggccgaga
     3001 tcctggacga gggctgggac gcgctgccgt ccggcgggcg actggtggtg cacagcgtga
     3061 ccgccgactc cgacgccgtg ctgctggccc tgcaccagcg ggtgggtggc gagctgagcc
     3121 ggatcgcggt cgaggtggcc gaaccgatcg gacgcttcac cggcttccgt ccgctgcgca
     3181 ccgtcacctg ctgggccgcc accaagggct gagtcggcac cgacgccggg gtgctggtcg
     3241 agctgacgac acctgcgagg gggcttcgac tcgaccagcg actgaccaac cgaccgcgac
     3301 ccgacggttt tgccgattcg cccgcgccga tgccggtgcg gtgacaggat cgaggtgccg
     3361 tccacgacac ggaacccggt gcgaatccgg ggcggtcggg ccactgtgat cccagcgatg
     3421 ggtagccaga tacgtgtgtg gacgacggtt ccttgggcca cgtcacctag aaagggaact
     3481 cgtcgatgca tatcgctgag ggattcctac ccgttgttca ctgcgtcgcc tggtacgcag
     3541 tctcggcccc gttcgtcatt cacggggcgc gcaaggtcat cgaagaggcg aaggagcacc
     3601 ccgaatcgcg gctgctgctc gccgccgccg gtgccttcac cttcgtgctg tcctcgatca
     3661 agctgccctc ggtcaccggc agctcgtcgc acccgaccgg caccggcctg ggcgcggtgc
     3721 tgttccgacc accggtgatg gccttcatgg ccaccgtcgt gctgatcttt caggcgctgc
     3781 tgctggcgca cggcggcatc accaccctgg gcgccaacgt gttcagcatg ggcatcgccg
     3841 gcccgtggat cggtttctgg tgctggcagt tgtcgaacaa gctgggctgg caccgcaacg
     3901 tcggcatgtt cctcgccctg gccttcgccg acctgtccac ctacgtcgtc accagcgtcc
     3961 aactgggcct ggccttcccc gacccggtct ccggtgtggt cggctcggtg gccaagttca
     4021 tgggcgtgtt cgccatctcg caggttccgc tggcgatcgc cgagggcatc cttggggtgc
     4081 tggtcttccg cgtgctgatc gacgtcgcga agcccgaact cgagcgtctc ggtgtgatca
     4141 agccggcggc gccggca
//
LOCUS       DAOSUG010000253         4149 bp    DNA     linear   ENV 10-JUN-2024
DEFINITION  MAG TPA_asm: Micropruina sp. isolate AMR_MDS_2195
            NODE_9041_length_4149_cov_4.298241, whole genome shotgun sequence.
ACCESSION   DAOSUG010000253 DAOSUG010000000
VERSION     DAOSUG010000253.1
DBLINK      BioProject: PRJNA925477
            BioSample: SAMN32864262
            Sequence Read Archive: SRR4236673
KEYWORDS    WGS; ENV; Metagenome Assembled Genome; MAG; Third Party Data; TPA;
            TPA:assembly.
SOURCE      Micropruina sp. (wastewater metagenome)
  ORGANISM  Micropruina sp.
            Bacteria; Bacillati; Actinomycetota; Actinomycetes;
            Propionibacteriales; Nocardioidaceae; Micropruina.
REFERENCE   1  (bases 1 to 4149)
  AUTHORS   Abdulkadir,N., Saraiva,J.P., Zhang,J., Stolte,S., Gillor,O.,
            Harms,H. and Rocha,U.
  TITLE     Genome-centric analyses of 165 metagenomes show that mobile genetic
            elements are crucial for the transmission of antimicrobial
            resistance genes to pathogens in activated sludge and wastewater
  JOURNAL   Microbiol Spectr 12 (3), e0291823 (2024)
   PUBMED   38289113
REFERENCE   2  (bases 1 to 4149)
  AUTHORS   Abdulkadir,N.
  TITLE     Direct Submission
  JOURNAL   Submitted (24-MAR-2023) Environmental Microbiology, Helmholtz
            Center for Environmental Research -UFZ, Permoserstr 15, Leipzig
            04318, Germany
COMMENT     The annotation was added by the NCBI Prokaryotic Genome Annotation
            Pipeline (PGAP). Information about PGAP can be found here:
            https://www.ncbi.nlm.nih.gov/genome/annotation_prok/
            
            ##Genome-Assembly-Data-START##
            Assembly Method        :: SPAdes v. 3.11.1
            Genome Representation  :: Full
            Expected Final Version :: Yes
            Genome Coverage        :: Not Applicable
            Sequencing Technology  :: Illumina MiSeq
            ##Genome-Assembly-Data-END##
            
            ##Genome-Annotation-Data-START##
            Annotation Provider               :: NCBI
            Annotation Date                   :: 05/04/2023 04:57:10
            Annotation Pipeline               :: NCBI Prokaryotic Genome
                                                 Annotation Pipeline (PGAP)
            Annotation Method                 :: Best-placed reference protein
                                                 set; GeneMarkS-2+
            Annotation Software revision      :: 6.5
            Features Annotated                :: Gene; CDS; rRNA; tRNA; ncRNA
            Genes (total)                     :: 2,776
            CDSs (total)                      :: 2,750
            Genes (coding)                    :: 2,714
            CDSs (with protein)               :: 2,714
            Genes (RNA)                       :: 26
            tRNAs                             :: 24
            ncRNAs                            :: 2
            Pseudo Genes (total)              :: 36
            CDSs (without protein)            :: 36
            Pseudo Genes (ambiguous residues) :: 0 of 36
            Pseudo Genes (frameshifted)       :: 2 of 36
            Pseudo Genes (incomplete)         :: 32 of 36
            Pseudo Genes (internal stop)      :: 2 of 36
            ##Genome-Annotation-Data-END##
FEATURES             Location/Qualifiers
     source          1..4149
                     /organism="Micropruina sp."
                     /mol_type="genomic DNA"
                     /submitter_seqid="NODE_9041_length_4149_cov_4.298241"
                     /isolate="AMR_MDS_2195"
                     /isolation_source="Activated sludge of municipal
                     wastewater treatment plants"
                     /db_xref="taxon:2737536"
                     /environmental_sample
                     /geo_loc_name="Canada"
                     /lat_lon="49.25 N 123.1 W"
                     /collection_date="2015-06-16"
                     /metagenome_source="wastewater metagenome"
                     /note="metagenomic"
     gene            <1..567
                     /locus_tag="PLF56_08895"
     CDS             <1..567
                     /locus_tag="PLF56_08895"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_020384784.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_function: GO:0005524 - ATP binding [Evidence IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="ATP-binding cassette domain-containing protein"
                     /protein_id="HOQ53715.1"
                     /translation="GVVTQEAHLFHDTIRMNLLYAKPSATDAELEAALRAAQVWRLVE
                     SLPEGLDTVVGDRGHRLSGGEKQRFAIARLLLKGPGLVVLDEATAHLDSESEAAVQRA
                     LDAALANRTSLVIAHRLSTVRGADHILVVAGGRIVEEGTHVDLLSRGGLYADLYRTQF
                     AEQADSRTAARSLLNGAVPSEDVPGGMS"
     gene            complement(673..2004)
                     /locus_tag="PLF56_08900"
     CDS             complement(673..2004)
                     /locus_tag="PLF56_08900"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_013160387.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_component: GO:0005886 - plasma membrane [Evidence IEA];
                     GO_function: GO:0015295 - solute:proton symporter activity
                     [Evidence IEA];
                     GO_process: GO:0015718 - monocarboxylic acid transport
                     [Evidence IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="gluconate:H+ symporter"
                     /protein_id="HOQ53716.1"
                     /translation="MSAQLIIAALAGIATIIVLIVWLKVHPFLALMGGSAVMALSAGV
                     PYADTFKSFLSGLGSTLSGVGVLIILGSMIGTLLVSSGGADTIVDTILDGTPVQRLPW
                     AMALIAFIVGIPLFFEVGIVILIPVVMFAARRAKLPVVLLGIPALAGLSALHGLVPPH
                     PGPLIAISALNANLGLTLGLGLLIAVPCVILAGPLLAKPMAKWVPHPAREDFLGKPPA
                     EGAKRPGFGASLTVILLPVALMLAATLVETTGIDKSPVGHVVAFIGTPLIALLITTVV
                     AMFVFGYALGKSLAEVGEQVGASFAPIAGILFIVGAGGGFKQTLVDSKIAEMLANALV
                     QAALPVLFSAWLVSVAIRLATGSATVATITASGIMAPLATGLSPSHVALMVLAIGAGS
                     VFLSHVNDAGFWMVKEYFGLSVGETFKTWSLMETVLSVIGLAGVMLLSLVL"
     gene            2994..>4149
                     /locus_tag="PLF56_08905"
     CDS             2994..>4149
                     /locus_tag="PLF56_08905"
                     /inference="COORDINATES: protein motif:HMM:NF014741.2"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="DUF222 domain-containing protein"
                     /protein_id="HOQ53717.1"
                     /translation="MEQYSDLTDSQLVAVIDAALDALTTRGPDLSNDRAQLAVFAAAI
                     RTQGRLQTFIHTRAAQLDTDEVAQRAHGTSLTTWAITTLTHTHREAATLVKAGEQLGR
                     FPTITNAAQAGAVSPAQADAITQVLTDLPDDLPASTLVQGEQLMLDFAATFNSTELRR
                     LAHHLLECLAPEVADQKEADRLERNHRHAQRTRHLTFTPDHHGSVFLKGSLPSPDAAT
                     LIALIDSYVTAQRGIDRLDPRTPTPTPGMRRADALMALVHAHSQAETAPKRAGDRPRA
                     TITLDYATLEATAKQLGLLQGRLGPTNEPIPGSVLRRLLCDADILPIILGSDGIPLDV
                     GRTQRLVTPTIRAALDQRDQGCVFPGCDKPPTNCHAHHIQPWWNGGTTALH"
BASE COUNT          793 a         1496 c         1278 g          582 t
ORIGIN      
        1 ggcgtggtca cgcaagaggc gcacctgttt cacgacacca tccgcatgaa cctgctgtac
       61 gccaagccgt ccgccaccga cgccgaactc gaggcggcgc tgcgcgcggc gcaggtgtgg
      121 cggctggtcg agtcgctgcc cgagggcctc gacacggtgg tcggcgatcg cggacaccgg
      181 ctctcggggg gcgagaagca gcggttcgcg atcgcccggc tgctgctcaa gggtccgggg
      241 ctggtggtgc tggacgaggc gaccgcccac ctcgacagcg agagcgaggc cgccgtccag
      301 cgcgcgttgg acgccgcact ggccaaccgc acgtccctgg tgatcgccca ccggctttcg
      361 accgtgcgcg gcgcagacca cattctcgtg gtcgcgggcg gacgcatcgt cgaggagggc
      421 acgcatgtgg acctgctcag tcgtggtgga ctgtacgccg acctgtaccg cacccagttc
      481 gcagagcagg ccgacagccg taccgccgct aggagtttgc tgaacggtgc tgttccgagc
      541 gaggacgtgc ccggcgggat gagctgagcc ccccagatct ggaggtcgta ttggacatac
      601 gggcccagat atggggggat cagatcgccc gccgggtgcg ccgcagccgg gacatgattg
      661 ttcagcaggc tcctagagaa cgaggctgag cagcatcacg cccgccagcc cgatcaccga
      721 cagcaccgtc tccatcagcg accaggtctt gaacgtttcg ccgacgctca gcccgaagta
      781 ctccttgacc atccagaaac cggcgtcgtt gacgtggctg agaaacaccg agcccgcacc
      841 gatcgccagc accatgaggg cgacatgtga gggcgagagt ccggtggcca gcggggccat
      901 gatgcccgag gcggtgatgg tggccaccgt ggccgaaccc gtggccagcc ggatcgcaac
      961 cgacaccagc caggctgaga acagcaccgg cagcgcggcc tgcaccagtg cgttggccag
     1021 catctcggcg atcttcgagt cgaccagggt ctgcttgaaa ccaccgccgg cgcccacgat
     1081 gaagagaatt cccgcgatcg gcgcgaacga ggcgcccacc tgctcgccga cttcggcgag
     1141 gctcttgccg agcgcgtagc cgaacacgaa catcgcgacc acggtggtga tcagcagggc
     1201 gatcagcggc gtaccgatga aggcgaccac gtggccgacg ggtgacttgt cgatgccggt
     1261 cgtctcgacc agggtggcgg ccagcatcaa cgcgaccggc agcagaatga cggtcagcga
     1321 cgccccgaat cccggacgct tcgcaccctc ggcgggcggc ttgccgagaa agtcctcgcg
     1381 ggccgggtgc ggcacccatt tcgccatcgg cttggcgagc agcgggcccg ccaggatcac
     1441 gcagggaact gcgatgagca gaccgagccc gagggtgagg ccgaggttcg cattgagtgc
     1501 cgagatggcg atcagggggc cggggtgcgg cggcaccagc ccgtgcaggg cggacagccc
     1561 ggccaaagca gggatgccga gcagcaccac cggtagcttc gcgcggcggg cagcgaacat
     1621 cacgaccggg atcagaatga cgatgccgac ttcgaagaac agcggaatgc cgacgatgaa
     1681 cgcgatcagt gccatggccc atggcagccg ctgcacgggc gtcccgtcga gaatggtgtc
     1741 gacgatggtg tcggccccac ccgacgagac cagtaacgtg ccgatcatcg atccgagaat
     1801 gatcaggacg cccactcccg acagggtcga gcccagcccg gagagaaaac tcttgaaggt
     1861 gtccgcgtag ggcacgcccg ccgacagcgc catgacggcc gagccgccca tcagcgcaag
     1921 gaacgggtgc accttgagcc agacgatcaa gacgatgatg gtggcgatgc cggcgagtgc
     1981 ggcgatgatg agctgagcgg acatgggcgg tgacttcctt gtcgtggccg ggggcggcca
     2041 agggtggtgc gcctcagtct gtcggcttgt cagcgcaaaa gcgatgggtg cgcgaccatt
     2101 cgaaacgcgg acgctgccct cgggtcagcc accgcaccga ttgcggctgc gtcggtcgtg
     2161 gccggtctct cttgtcgccc cggttggtgc gctgggttgc cgcggagtgg ggtgcacccc
     2221 ggccgcccgc accatcagcc tcaccggctc cgcagtgagt tgtccgacat ccgaccactc
     2281 ccggacgggt gccctcactc cgcttcaatc cggctttggg tgcctggcat tctcaaagtt
     2341 cgagcttgtc gagacccccg ccacatcgag gagcctgcgg aacacgaata tcgcgaccac
     2401 ggtggtgatc agcagggcga tcagcggcgt accgatgaag ggcgaccacg tggccgacgg
     2461 gtgacttgtc gatgccggtc gtctcgacca gcgtggcggc cagcatcaac gcgagcggca
     2521 gcagaatgac ggtcagcgac gccccgaatc ccggacgctt cgcaccctcg gcgggcggct
     2581 tgccgagtct cggctgcggc gcacccggcg ggtgatctga gccctccgta tctgggcccg
     2641 tatgtcctat acgacctcca gatctggggg gctcagctca tcccgccggg cacgtcctcg
     2701 ctcggaacag cattgttcag cagactccga gccgaccgat gccgcgctgc cggcagccac
     2761 caatcaccgc ggacgcgttc tggatccaaa tgtggccggt caaccgcgta gttaccgatg
     2821 ccttggcgcc ggcatcgcgg gaggtatccg ctgtcgccca gcatgacgtc cgaacccgtt
     2881 cgattgccaa ccaccaagac ctgcgtggat aacgcaaata tctatctaca tccttgccga
     2941 gaaggtgagc gttgttcgaa aaagtgtcag aggcggtctg tagcgtgaat gtcgtggagc
     3001 agtacagcga ccttaccgac agccaactgg tggccgtgat cgatgctgcc ctggatgcgc
     3061 tgaccacccg ggggccggac ctgtccaacg atcgtgccca gttggcggtg ttcgcggccg
     3121 cgatccgcac ccagggacga ctgcaaacct tcatccacac ccgcgccgca caactcgata
     3181 ccgacgaggt cgcccaacgc gcccatggca cctccctgac cacctgggcg atcaccaccc
     3241 tgacccacac ccaccgcgaa gcagccacac tcgtcaaagc cggtgagcaa ctcggccggt
     3301 tccccaccat caccaacgcc gcacaagccg gtgccgtgtc accggcccag gccgacgcga
     3361 tcacccaggt actcaccgac ctacccgacg acctacccgc ctccacactg gtgcaaggcg
     3421 aacagctgat gctcgacttc gccgccacct tcaactccac cgaactacgc cgactcgccc
     3481 accacctact cgaatgcctc gcccccgaag tcgccgacca gaaagaagcc gaccggctcg
     3541 aacgcaacca ccggcacgcc caacgcaccc ggcacctgac cttcaccccc gaccaccacg
     3601 gatcggtgtt cctcaaaggc agcctgccca gccccgacgc cgccaccctc atcgccctga
     3661 tcgactccta cgtcaccgcg caacgcggca tcgaccgact cgacccacgc acacccaccc
     3721 ccacccccgg catgcgacgc gctgatgccc tgatggcact cgtccacgcc cacagccaag
     3781 ccgaaactgc cccgaaacgg gccggtgacc ggccccgcgc caccatcacc ctcgactacg
     3841 ccacactcga agccaccgcc aaacaactcg gactactcca aggccggctc ggccccacca
     3901 acgaacccat ccccggcagc gtgctacgcc gcctgctctg cgacgccgac atcctgccca
     3961 tcatcctcgg cagcgacggc atacccctcg acgtcggccg aacccaacga ctcgtcaccc
     4021 ccaccatccg cgccgccctc gaccaacgcg accaaggctg cgtcttcccc ggctgcgaca
     4081 aaccacccac caactgccac gcccaccaca tccaaccctg gtggaacggc ggcaccaccg
     4141 ccctacaca
//
LOCUS       DAOSUG010000254         4141 bp    DNA     linear   ENV 10-JUN-2024
DEFINITION  MAG TPA_asm: Micropruina sp. isolate AMR_MDS_2195
            NODE_9057_length_4141_cov_3.799804, whole genome shotgun sequence.
ACCESSION   DAOSUG010000254 DAOSUG010000000
VERSION     DAOSUG010000254.1
DBLINK      BioProject: PRJNA925477
            BioSample: SAMN32864262
            Sequence Read Archive: SRR4236673
KEYWORDS    WGS; ENV; Metagenome Assembled Genome; MAG; Third Party Data; TPA;
            TPA:assembly.
SOURCE      Micropruina sp. (wastewater metagenome)
  ORGANISM  Micropruina sp.
            Bacteria; Bacillati; Actinomycetota; Actinomycetes;
            Propionibacteriales; Nocardioidaceae; Micropruina.
REFERENCE   1  (bases 1 to 4141)
  AUTHORS   Abdulkadir,N., Saraiva,J.P., Zhang,J., Stolte,S., Gillor,O.,
            Harms,H. and Rocha,U.
  TITLE     Genome-centric analyses of 165 metagenomes show that mobile genetic
            elements are crucial for the transmission of antimicrobial
            resistance genes to pathogens in activated sludge and wastewater
  JOURNAL   Microbiol Spectr 12 (3), e0291823 (2024)
   PUBMED   38289113
REFERENCE   2  (bases 1 to 4141)
  AUTHORS   Abdulkadir,N.
  TITLE     Direct Submission
  JOURNAL   Submitted (24-MAR-2023) Environmental Microbiology, Helmholtz
            Center for Environmental Research -UFZ, Permoserstr 15, Leipzig
            04318, Germany
COMMENT     The annotation was added by the NCBI Prokaryotic Genome Annotation
            Pipeline (PGAP). Information about PGAP can be found here:
            https://www.ncbi.nlm.nih.gov/genome/annotation_prok/
            
            ##Genome-Assembly-Data-START##
            Assembly Method        :: SPAdes v. 3.11.1
            Genome Representation  :: Full
            Expected Final Version :: Yes
            Genome Coverage        :: Not Applicable
            Sequencing Technology  :: Illumina MiSeq
            ##Genome-Assembly-Data-END##
            
            ##Genome-Annotation-Data-START##
            Annotation Provider               :: NCBI
            Annotation Date                   :: 05/04/2023 04:57:10
            Annotation Pipeline               :: NCBI Prokaryotic Genome
                                                 Annotation Pipeline (PGAP)
            Annotation Method                 :: Best-placed reference protein
                                                 set; GeneMarkS-2+
            Annotation Software revision      :: 6.5
            Features Annotated                :: Gene; CDS; rRNA; tRNA; ncRNA
            Genes (total)                     :: 2,776
            CDSs (total)                      :: 2,750
            Genes (coding)                    :: 2,714
            CDSs (with protein)               :: 2,714
            Genes (RNA)                       :: 26
            tRNAs                             :: 24
            ncRNAs                            :: 2
            Pseudo Genes (total)              :: 36
            CDSs (without protein)            :: 36
            Pseudo Genes (ambiguous residues) :: 0 of 36
            Pseudo Genes (frameshifted)       :: 2 of 36
            Pseudo Genes (incomplete)         :: 32 of 36
            Pseudo Genes (internal stop)      :: 2 of 36
            ##Genome-Annotation-Data-END##
FEATURES             Location/Qualifiers
     source          1..4141
                     /organism="Micropruina sp."
                     /mol_type="genomic DNA"
                     /submitter_seqid="NODE_9057_length_4141_cov_3.799804"
                     /isolate="AMR_MDS_2195"
                     /isolation_source="Activated sludge of municipal
                     wastewater treatment plants"
                     /db_xref="taxon:2737536"
                     /environmental_sample
                     /geo_loc_name="Canada"
                     /lat_lon="49.25 N 123.1 W"
                     /collection_date="2015-06-16"
                     /metagenome_source="wastewater metagenome"
                     /note="metagenomic"
     gene            <1..375
                     /locus_tag="PLF56_08910"
     CDS             <1..375
                     /locus_tag="PLF56_08910"
                     /inference="COORDINATES: protein motif:HMM:NF013786.2"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_function: GO:0000287 - magnesium ion binding [Evidence
                     IEA];
                     GO_function: GO:0008897 - holo-[acyl-carrier-protein]
                     synthase activity [Evidence IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="4'-phosphopantetheinyl transferase superfamily
                     protein"
                     /protein_id="HOQ53718.1"
                     /translation="ERADAAGFDGFAAVALHPAEPVPHDATEATRLWVRKEAVLKALG
                     SGLRLDPRELRLAGCLDSTGANRSSEKPRVLAWPDPRPPSIVELCDLDAPAGYLAALA
                     VIGGRGAAYTQAAIRLDTSSGV"
     gene            complement(288..1583)
                     /locus_tag="PLF56_08915"
     CDS             complement(288..1583)
                     /locus_tag="PLF56_08915"
                     /EC_number="3.4.11.-"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_017932807.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_function: GO:0004177 - aminopeptidase activity
                     [Evidence IEA];
                     GO_function: GO:0008237 - metallopeptidase activity
                     [Evidence IEA];
                     GO_function: GO:0008270 - zinc ion binding [Evidence IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="M1 family metallopeptidase"
                     /protein_id="HOQ53719.1"
                     /translation="MPAADPYVPGHGNRRFSVRHYALDLDYKIATNHLGARAALEIEA
                     LEPLDRVVLDLAGLNVSRVRVNGRAPARFTHSDRKLRIVLSAPAASGERLSVEIDYAG
                     KPKPMPSVFGPVGWEELTDGVLVAAQPCGAPSWFPCNDRPDDKATYRFAITVAEGYRV
                     VANGVLAGREKRAGRITWRFLQSQPMAPYLAVLHIGRYVSTPAGEAEIVHPRGLGVGA
                     GSAFARQADMLTVFSELFGPYPFDRYSAVITADALEIPLEAQTLSTFGINHARPGWQN
                     ERLVAHELAHQWFGNSLTAASWADIWLHEGFACYSEWLWSEASGGRSADSWAQEHHRR
                     LTGLPQDLVLGDPGREAMFDDWVYKRGALTLHALRGLLGEAFFTMLRDWTAAHRWGVV
                     STADFTTHAQRYTPLDVSSLIAAWVYAAPLPPMTANAAR"
     gene            complement(1583..>4141)
                     /locus_tag="PLF56_08920"
     CDS             complement(1583..>4141)
                     /locus_tag="PLF56_08920"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_007077667.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="phosphopantetheine-binding protein"
                     /protein_id="HOQ53720.1"
                     /translation="VAVEPGFDAHESLALLRERLPAPLVPRLAVVPELPTRTSGKIDR
                     DALPWPLPESDAGASELQVSGTRAMVRDHWEAVLSTRVTALEADFFDLGGGSLTAAQM
                     VSLLRRTHPEVTVADIYDHPTLAALADHLDEMATPTSQLNANVLPVPPKTQRGQLLAL
                     LPLRTIAGLRWAIWTAAACNLAHAAGVSWLPTLSWWWVLFGAALMILTPGRMLVTALG
                     VRLILAGIGPGSYPRGGKVHLRLWLAERFADEMGSESIAGAVLLRWYATLLGATVEPH
                     VDLHSAPPVTGLLRLGEGCSIEPEVDLAGHWLDGGQLIIGEIRVGAGARVGARSTLCP
                     GADIGPEAEVAPGSSVFGAVGAGEYWSGSPAVKVRERARGPWPQRPNNQPAWLIGYAA
                     MGVLIAGLPLLATLAGFAVLWPALRASTSLGDALWAALPWLVPATLVGYGVLTLLVWV
                     LVRLLALGLKTGPHPVQSRNSWQAWSTIRVLDEARTWLFPLYASFATPGWLRALGARI
                     GTNVEASTVLMIPSLVTVADGAFLADDTLIGGYELGGGWLRIDEVRIGKHAFVGNSGM
                     AAPGRKVPKGGLVAVLSAAPARRRAKRGTSWIGSPPTKLRRSAGSADESRTYAPATRL
                     KVARALVEVCRLFAPLTLVTLTTLCVAALVGLGQVSWWLAGVASGVVLGVGGLLAAAS
                     ASVAKWVLLGRLRASDHPLWSSLVWRNELADMFVELLAAPWFAHPTMGTPALNAWLRT
                     MGAKVGQGVWCETYWLPESDLIDLRAGVSVNRGCVVQTHLFHDRVLSMDTVTLRAGAT
                     LGPNSVILPAATIGRHATIGPVSLVMRGESVPDKTRWIGNPIAPWPATEPTTET"
BASE COUNT          652 a         1506 c         1455 g          528 t
ORIGIN      
        1 gaacgcgcgg acgccgccgg cttcgacggg ttcgccgccg tcgcgctgca ccccgccgag
       61 ccggtcccgc atgacgccac ggaagcgacg cggctctggg tacgcaagga ggcggtactc
      121 aaggcactgg gcagcgggct acggctcgac ccgcgcgaac tccgcctggc gggctgcctg
      181 gactcgacag gcgcgaacag gtctagcgag aagccccgcg tgctcgcgtg gcccgacccg
      241 cgcccgccgt ccatcgtcga gctgtgcgat ctggacgccc cggcgggtta cctcgccgcg
      301 ttggccgtca tcggcggcag gggagcggcg tacacccagg cggcgatcag gctcgacacg
      361 tcgagcgggg tgtagcgctg cgcgtgcgtg gtgaagtccg cggtgctcac cacaccccag
      421 cggtgcgcgg ccgtccagtc gcgcagcatc gtgaagaacg cctcgccgag cagcccacgc
      481 agggcatgca gggtgagcgc cccgcgcttg tacacccagt cgtcgaacat cgcctcgcgg
      541 cccggatcgc ccagcaccag atcctgcggc agcccagtca gccgacgatg gtgctcctgc
      601 gcccacgagt cggcactgcg gccacccgat gcctcggacc acagccattc gctgtagcag
      661 gcgaatccct cgtgcagcca gatgtcggcc cagctggccg cggtgaggct gttgccgaac
      721 cactggtggg ccagttcgtg ggcgaccagc cgctcgttct gccagccggg ccgggcatgg
      781 ttgatgccga acgtcgacag ggtctgagcc tccagcggaa tctccagggc atccgcggtg
      841 atcaccgccg agtaccggtc gaacgggtag ggcccgaaga gctcactgaa cacggtcagc
      901 atgtcggcct gtcgtgcgaa ggccgagccc gcacccaccc cgaggccgcg cggatgcacg
      961 atctcggcct cgccggccgg cgtgctgacg tagcgtccga tgtgcagcac ggccaggtac
     1021 ggcgccatcg gctgcgattg cagaaagcgc caagtgatgc gtccggcccg cttctcacgg
     1081 ccggcgagca ccccgttggc gaccacccgg tagccctcgg ccacggtgat cgcgaagcgg
     1141 taggtggcct tgtcgtccgg ccggtcgttg cacggaaacc aggacggcgc cccgcacggc
     1201 tgggccgcga ccagcacccc gtcggtcagt tcctcccagc ccaccggccc gaacacgctc
     1261 ggcatcggct tgggtttgcc cgcatagtcg atctcgacac tgaggcgttc accggacgcg
     1321 gcgggcgcgc tgagcacgat ccgcagcttg cggtcgctgt gggtgaaccg ggccggggcg
     1381 cgtccgttca ccctgacccg cgacacgttc aaaccggcca ggtcgagcac cacccggtcg
     1441 agcggctcga gggcctcgat ctcgagcgcc gcccgggcgc ccaggtggtt ggtggcgatc
     1501 ttgtagtcga ggtcgagcgc ataatggcgc acgctgaacc gacggttccc gtggccggga
     1561 acgtacgggt cggcagcagg catcaggtct ccgtggtcgg ctcggtcgcc ggccacgggg
     1621 cgatcgggtt accgatccat cgggtcttgt cagggacgga ttcaccgcgc atgaccagcg
     1681 ataccggccc gatcgtagcg tgtctaccga tggtggcggc gggcagaatg acgctgttcg
     1741 ggccgagcgt ggcgcccgcg cgcagggtga ccgtgtccat gctgagcacc cgatcgtgaa
     1801 acaggtgggt ctgcaccacg cagccccggt tcacgctcac cccggcgcgc aggtcgatca
     1861 ggtcggattc gggcagccag tacgtttcgc accacacccc ctggccgacc ttggcgccca
     1921 tcgtgcgcag ccacgcgttc agggcggggg tgcccatggt ggggtgcgcg aaccagggcg
     1981 cggccagcag ctcgacgaac atgtcggcga gctcgttgcg ccacaccaac gaactccaca
     2041 acggatgatc cgaggcgcgc agccggccca gcagcaccca tttcgccacg gaggcggacg
     2101 ctgccgcgag cagcccgccg acgcccagca ccacgcccga tgcgaccccg gccagccacc
     2161 agctcacctg ccccaggccg accaacgccg ccacgcacag ggtggtcagg gtgaccagcg
     2221 tgagcggagc gaacagccgg cagacctcga ccagagcgcg ggcaaccttc aaccgggtcg
     2281 ccggggcgta ggtgcgggat tcgtccgcgc tgccggcgct gcgccgaagc ttggtcggcg
     2341 gcgatccgat ccaggacgtg ccgcgcttgg cccggcgccg cgccggggcc gcggacaaca
     2401 ccgcgaccag gccgcccttg ggcaccttgc gcccgggcgc cgccatgccg gagttgccga
     2461 cgaaggcgtg cttgccgatc cgcacctcgt cgatgcgcag ccagccgccg ccgagttcgt
     2521 aaccgccgat cagggtgtcg tcggccagaa acgcgccgtc cgccacggtg accaggctcg
     2581 ggatcatcaa gaccgtggac gcctcgacgt tcgtgccgat ccgggcaccc agcgcacgca
     2641 gccagccggg tgtggcgaag ctggcataga gcgggaacaa ccaggtgcgc gcctcgtcca
     2701 gcacccggat cgtcgaccat gcctgccacg agttgcgcga ctggacgggg tgcggccccg
     2761 ttttcaggcc gagcgccagc aggcgcacca gcacccagac cagcagcgtg agcaccccgt
     2821 agccgaccag ggtggcgggc accagccagg gcagtgcggc ccacagggcg tccccgagcg
     2881 aggtactggc gcgcagggcc ggccacagca ccgcgaaccc ggccagcgtg gccagcaacg
     2941 gcagcccggc gatcagcaca cccatcgccg cgtagccgat caaccacgcc ggctggttgt
     3001 tcggacgctg cggccacggc ccgcgcgcgc gctcacgcac cttgaccgcc ggtgagcccg
     3061 accagtactc gccggcgccg accgcaccga agacggacga accgggtgcg acttcggcct
     3121 ccggcccgat gtcggcgccc gggcacagcg tggagcgggc accgacgcgg gcgcccgcgc
     3181 cgacccggat ctcgccgatg atcaactggc cgccgtccag ccagtgaccg gccaggtcga
     3241 cctcgggctc gatcgaacag ccctcgccca gccgcagcag ccccgtcacc ggcggcgcgc
     3301 tgtgcaggtc gacatgcggt tcgacggtgg cgccgagcaa ggtggcgtac cagcgcagca
     3361 acaccgcgcc cgcgatcgat tcgctgccca tctcgtcggc gaaccgctcg gccagccaca
     3421 accgcaggtg caccttgccg ccgcgcgggt agctgccggg accgatcccg gccaggatca
     3481 gccgcacgcc cagtgcggtg accagcatgc gtccgggggt gaggatcatc agggctgcgc
     3541 cgaacagcac ccaccaccac gacagggtcg gcagccaact caccccggcg gcgtgggcca
     3601 ggttgcaggc ggcggccgtc cagatcgccc agcgcaggcc cgcgatcgtg cgcagcggca
     3661 gcagggccag cagttgaccg cgctgggtct tcggcggcac gggtaatacg ttcgcgttca
     3721 gttgcgaggt gggggtcgcc atctcgtcca ggtgatcggc cagggcggcc agcgtggggt
     3781 ggtcgtagat gtcggcgacc gtcacctcgg ggtgggtgcg acgcaacagc gacaccatct
     3841 gggccgcggt cagcgaaccg ccacccaggt cgaagaagtc ggcctccagc gcagtgacac
     3901 gggtgctgag caccgcctcc cagtggtcgc gcaccatggc ccgggtgccg ctcacctgca
     3961 gctcggacgc ccccgcgtcc gattccggca ggggccaggg cagcgcgtcg cggtcgatct
     4021 tgcccgaggt gcgggtgggc agctcgggca ccacggcgag ccgcggcacc aggggagcgg
     4081 gcagtcgttc gcgcagcagg gccagcgatt cgtgggcgtc gaaaccgggc tcgacggcga
     4141 c
//
LOCUS       DAOSUG010000255         4123 bp    DNA     linear   ENV 10-JUN-2024
DEFINITION  MAG TPA_asm: Micropruina sp. isolate AMR_MDS_2195
            NODE_9105_length_4123_cov_4.436578, whole genome shotgun sequence.
ACCESSION   DAOSUG010000255 DAOSUG010000000
VERSION     DAOSUG010000255.1
DBLINK      BioProject: PRJNA925477
            BioSample: SAMN32864262
            Sequence Read Archive: SRR4236673
KEYWORDS    WGS; ENV; Metagenome Assembled Genome; MAG; Third Party Data; TPA;
            TPA:assembly.
SOURCE      Micropruina sp. (wastewater metagenome)
  ORGANISM  Micropruina sp.
            Bacteria; Bacillati; Actinomycetota; Actinomycetes;
            Propionibacteriales; Nocardioidaceae; Micropruina.
REFERENCE   1  (bases 1 to 4123)
  AUTHORS   Abdulkadir,N., Saraiva,J.P., Zhang,J., Stolte,S., Gillor,O.,
            Harms,H. and Rocha,U.
  TITLE     Genome-centric analyses of 165 metagenomes show that mobile genetic
            elements are crucial for the transmission of antimicrobial
            resistance genes to pathogens in activated sludge and wastewater
  JOURNAL   Microbiol Spectr 12 (3), e0291823 (2024)
   PUBMED   38289113
REFERENCE   2  (bases 1 to 4123)
  AUTHORS   Abdulkadir,N.
  TITLE     Direct Submission
  JOURNAL   Submitted (24-MAR-2023) Environmental Microbiology, Helmholtz
            Center for Environmental Research -UFZ, Permoserstr 15, Leipzig
            04318, Germany
COMMENT     The annotation was added by the NCBI Prokaryotic Genome Annotation
            Pipeline (PGAP). Information about PGAP can be found here:
            https://www.ncbi.nlm.nih.gov/genome/annotation_prok/
            
            ##Genome-Assembly-Data-START##
            Assembly Method        :: SPAdes v. 3.11.1
            Genome Representation  :: Full
            Expected Final Version :: Yes
            Genome Coverage        :: Not Applicable
            Sequencing Technology  :: Illumina MiSeq
            ##Genome-Assembly-Data-END##
            
            ##Genome-Annotation-Data-START##
            Annotation Provider               :: NCBI
            Annotation Date                   :: 05/04/2023 04:57:10
            Annotation Pipeline               :: NCBI Prokaryotic Genome
                                                 Annotation Pipeline (PGAP)
            Annotation Method                 :: Best-placed reference protein
                                                 set; GeneMarkS-2+
            Annotation Software revision      :: 6.5
            Features Annotated                :: Gene; CDS; rRNA; tRNA; ncRNA
            Genes (total)                     :: 2,776
            CDSs (total)                      :: 2,750
            Genes (coding)                    :: 2,714
            CDSs (with protein)               :: 2,714
            Genes (RNA)                       :: 26
            tRNAs                             :: 24
            ncRNAs                            :: 2
            Pseudo Genes (total)              :: 36
            CDSs (without protein)            :: 36
            Pseudo Genes (ambiguous residues) :: 0 of 36
            Pseudo Genes (frameshifted)       :: 2 of 36
            Pseudo Genes (incomplete)         :: 32 of 36
            Pseudo Genes (internal stop)      :: 2 of 36
            ##Genome-Annotation-Data-END##
FEATURES             Location/Qualifiers
     source          1..4123
                     /organism="Micropruina sp."
                     /mol_type="genomic DNA"
                     /submitter_seqid="NODE_9105_length_4123_cov_4.436578"
                     /isolate="AMR_MDS_2195"
                     /isolation_source="Activated sludge of municipal
                     wastewater treatment plants"
                     /db_xref="taxon:2737536"
                     /environmental_sample
                     /geo_loc_name="Canada"
                     /lat_lon="49.25 N 123.1 W"
                     /collection_date="2015-06-16"
                     /metagenome_source="wastewater metagenome"
                     /note="metagenomic"
     gene            complement(<1..492)
                     /locus_tag="PLF56_08925"
     CDS             complement(<1..492)
                     /locus_tag="PLF56_08925"
                     /inference="COORDINATES: protein motif:HMM:NF012965.2"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_function: GO:0004488 - methylenetetrahydrofolate
                     dehydrogenase (NADP+) activity [Evidence IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="tetrahydrofolate dehydrogenase/cyclohydrolase
                     catalytic domain-containing protein"
                     /protein_id="HOQ53721.1"
                     /translation="MSAVMMPGQPVADAVFADLQPRIAALRAAGHNPGLGTILVGGDA
                     ASAGYIRMKQQRASDLGFGGRHVQLPGDASQADLVAVIRDFNTDDEVDAVLIQHPIPA
                     HLDYEAALAELDPDKDADGAHPTNMGRLALGMKGPLPCTPAGIEALLQFYDIAVAGRD
                     VVIL"
     gene            complement(691..2385)
                     /locus_tag="PLF56_08930"
     CDS             complement(691..2385)
                     /locus_tag="PLF56_08930"
                     /inference="COORDINATES: protein motif:HMM:NF016202.2"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="VanW family protein"
                     /protein_id="HOQ53722.1"
                     /translation="MSARNLALWITGGAVALTAGTYVAGYAMAGDNTPRNASVSGVPI
                     GGLSAEAATTKLQAELAPRLTRPLTVQAGSEQVQTTAKKLGLGVDYAASVSNAGVGRS
                     WHPAHIISVLTGGGEQPAVVTRDAAVLDAALAGLAEQADRPPVNAGVSVTKGAIERAE
                     SEPGVSVQQAQTGDALADGFLTRTTIAAVTSSTEPEVTTAEADQVVTQVGKPALAAPI
                     TVRTGSSGSFELTPAMIGAALTFEPADGTLKPVLNPEKLTTAAAKTIKGLGLKQPKNA
                     DITIANGKPKIIASVDGIGLKPDDLAKAALGVLESPKPRNVTVEATVQKAHFTTADAE
                     KLGVKQVTGSFTTYYPGSAYRVNNIGKAARLINGTFLKPGETFSMNAALGQRTAAAGW
                     MAGGGIEGGKIKTLLGGGISQATTTTFNAIFFAGLEDVYHKPHSLYFSRYPVGREATL
                     DWVSVDMKFRNDSPYGVVLQAGTTGKVGQQGSVTVKVWSTKRYTIKTTTPVRSNFRAP
                     GPTQYDESPGCVAQSAMSGFDVSYKRLFYADGKLVKSEPFFWRYNTLTPVVCGKKP"
     gene            complement(2410..>4123)
                     /locus_tag="PLF56_08935"
     CDS             complement(2410..>4123)
                     /locus_tag="PLF56_08935"
                     /inference="COORDINATES: protein motif:HMM:NF013506.2"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=2
                     /transl_table=11
                     /product="S49 family peptidase"
                     /protein_id="HOQ53723.1"
                     /translation="LFRTSAHGDRVILELDLDFSVLSSAPANPFEALKLINAPTMRAL
                     RDGLHAAAADEAVVGLIVHIGTCPVSASQASELADSIAGFGEHKPVWAYTESFGEFGT
                     ALLPYVLAAHADQVWLQPSGELGLGGVRAQITLLRGTLDKVGLEPQFSQRKEYKSAAD
                     TYAAREITEANHEMVQQIANSLTHWAVDLIAQRRSRTPEQVWASVDAGILTPPQAVEH
                     GLVDQIGYRDQVYAAARAAWGGEQAQLRFVHRYARVSAAKELVQAKLKPAVAVVEIRG
                     GIVAGRPPRQGGQPVAASEVVCQHLRAAAADESVKAVVLRVESPGGSYIASDVIRREV
                     LRLRESGRPVVACMGDVAGSGGYFVAMPANEIVAQPTTLTGSIGVLAGKFVTTELKNK
                     IGLVMADVISGPWSSFLSPNQPFTEQQWAALDQRLDDIYADFTGKAAIDRHLALEVLE
                     PVARGRVWTGVDARERGLIDHLGGMNLAIDRACALASVKRSDVAVRPVSMLGFLKQFK
                     PAESSESVATQVTMSRRLSVDGVIEAFAGMLGVQAHGALAMPFQVALHAGLGSPVVYD
                     GSDA"
BASE COUNT          597 a         1439 c         1415 g          672 t
ORIGIN      
        1 cagaatcacc acgtcgcgac ccgcgaccgc aatgtcgtag aactgcagca gcgcctcgat
       61 gccggccggc gtgcacggca gggggccttt catgccaagg gcgagccggc ccatgttggt
      121 cgggtgggca ccgtccgcgt ccttgtcggg atcgagctcg gccagcgccg cctcgtagtc
      181 gaggtgggcc ggaatcgggt gctggatcag taccgcgtcc acctcgtcat cggtgttgaa
      241 gtcgcggatc acggcgacca ggtcggcctg cgaggcgtcc ccgggcagtt ggacgtgccg
      301 cccgccgaaa cccaggtcgc tcgcccgctg ctgcttcatc cgaatgtagc cggcggacgc
      361 ggcatcgccg cccaccagaa tcgtgccgag ccccgggttg tgccccgcgg cccgaagcgc
      421 cgcgatccgg ggctggaggt cggcgaaaac ggcatcggca accggttggc ccggcatcat
      481 cactgcgctc acgtcgcctg agcgtacgcg ctcggcccgg cgtgccgggg cgatcttcgc
      541 ctacgagcgc caccatgaag ggttggccgc tgagcggacg gctgatctct cgctggtcga
      601 gctacgaccc cagcacctac ctgatcgaga gcgtcaagcc cgcgtgaacc gggtttcgac
      661 ccgttcgacc aacggaccga aagcggcgcc tcacggcttc ttgccgcaga ccaccggggt
      721 gagggtgttg taccgccaga agaacggctc ggacttgacc agcttgccgt cggcgtagaa
      781 caaccgcttg tagctcacgt cgaagccgct catcgcgctc tgcgccacac agccgggcga
      841 ctcgtcgtac tgcgtcgggc cgggtgcacg gaagttgctg cgcaccggcg tggtggtctt
      901 gatcgtgtag cgcttggtcg accacacctt caccgtcacc gaaccctgct ggccgacctt
      961 gccggtggtc ccggcctgta ataccacgcc gtagggcgag tcgttgcgaa acttcatgtc
     1021 gacgctgacc cagtcgaggg tcgcctcgcg tccgaccggg tagcggctga agtacaacga
     1081 atgcggcttg tggtagacgt cttcgaggcc ggcgaagaag atcgcattga acgtggtggt
     1141 ggtcgcctgg ctgatgcccc caccgagcag ggtcttgatc ttgccgccct cgatgccgcc
     1201 gcccgccatc cagccggccg cagccgtgcg ttggcccagc gcggcgttca tgctgaacgt
     1261 ctcccccggc ttgaggaagg tgccgttgat cagccgggcg gccttgccga tgttgttcac
     1321 ccggtaggcg gagccggggt aatacgtggt gaacgagccg gtcacctgct tgacgccgag
     1381 cttctcggca tcggcggtgg tgaagtgggc cttctgcacc gtcgcctcga cggtcacgtt
     1441 gcgcggcttg ggcgattcga gcacgccgag cgcagctttg gccaggtcgt cgggcttcag
     1501 gccgataccg tcgaccgacg caatgatctt cggcttgccg ttcgcgatcg tgatgtcggc
     1561 gttcttgggt tgcttcaggc ccaacccctt gatcgtcttg gcggcggcgg tggtcagctt
     1621 ctcggggttc agcaccggct tgagggtgcc gtcggcgggc tcgaacgtaa gcgcagcgcc
     1681 gatcatggcc ggggtcagct cgaacgaacc ggacgacccg gtgcgcaccg tgatcggtgc
     1741 ggcgagcgcc ggcttgccga cctgggtcac gacctggtcg gcctcggcgg tggtgacctc
     1801 gggttcggtg ctgctggtca cggccgcaat ggtggtgcgg gtcagaaagc cgtccgcgag
     1861 cgcgtcgccg gtctgtgcct gctgcacgct cacgccgggc tcgctctcgg cgcgttcgat
     1921 cgcacccttc gtcacgctca ccccggcgtt caccggcgga cggtcggcct gttcggccag
     1981 gccggccagc gcggcgtcca gcacggcggc gtcgcgggtg accaccgccg gctgctcacc
     2041 gcccccggtg agcacgctga tgatgtgcgc gggatgccag ctgcgtccga ccccggcgtt
     2101 gctgacgctg gccgcgtagt cgacgccaag gccgagcttc ttcgcggtgg tttgcacctg
     2161 ctcgctgccc gcctggacgg tgagcggccg ggtcagccgg ggcgccagct cggcctgcag
     2221 cttcgtggtc gccgcctcgg cactcaaccc tccgatcggc acccccgaca ccgatgcgtt
     2281 gcgcggcgtg ttgtcgccgg ccattgcgta gccggccacg taggtacccg cggtcagcgc
     2341 cacggcaccc ccggtgatcc acagggcaag gtttcgggcg ctcacaggtg tctacctcga
     2401 tcgtcggggt caggcatctg acccatcgta aacgaccggg ctgcccagcc ctgcgtgcag
     2461 ggcgacctgg aacggcatcg ccagggcgcc gtgcgcctgc acgcccagca tgccggcgaa
     2521 ggcctcgatc acgccgtcca cgctgagccg gcgcgacatg gtcacctgcg tggccaccga
     2581 ttcgctcgac tcggccggct tgaactgctt caggaaaccg agcatcgaca ccggacgcac
     2641 cgccacgtcc gagcgcttga cggacgccaa cgcgcacgcc cggtcgatgg ccaggttcat
     2701 gccgccgagg tggtcgatca ggcctcgctc gcgggcgtcc acccccgtcc agacccgtcc
     2761 gcgggcgacc ggctcgagta cctcgagggc caggtgacga tcgatggcgg ccttgccggt
     2821 gaagtcggcg tagatgtcgt cgaggcgctg gtcgagcgcc gcccactgct gctcggtgaa
     2881 gggctggttg ggcgacagga acgacgacca ggggcccgag atcacatcgg ccatcaccag
     2941 accgatcttg ttcttcagct cggtggtcac gaacttgccc gccagcacgc cgatcgagcc
     3001 ggtcagcgtg gtcggctgcg cgacgatctc gttggccggc atggccacga agtacccgcc
     3061 cgagccggcc acgtcaccca tgcacgccac caccggacgt ccggactcac gcagcctgag
     3121 cacctcacgc cggatcacgt cggaggcgat gtacgagccg cccggcgact cgacccgcag
     3181 cacgacggcc ttgaccgact cgtccgcggc cgccgcccgc aggtgctgac agaccacctc
     3241 ggacgccgcc accggctgcc caccctggcg gggcgggcgt ccggccacga ttccgccccg
     3301 gatctcgacc actgcgacgg ccggcttgag cttggcctgc acgagctcct tggcggccga
     3361 gacccgcgcg tagcggtgca cgaagcgcag ttgcgcctgc tcgccgcccc aggccgcacg
     3421 ggccgcagcg tagacctggt cgcggtagcc gatctggtcc accagaccgt gctccacggc
     3481 ctgcggcggg gtcagaatgc cggcatcgac gctggcccag acctgctccg gcgtgcgcga
     3541 gcgacgttgc gcgatgaggt cgaccgccca atgcgtcagt gagttggcga tctgctgcac
     3601 catctcgtgg ttcgcttcgg tgatctcgcg ggccgcgtag gtgtcggcgg cgctcttgta
     3661 ttccttgcgc tgcgagaact gcggttcgag accgaccttg tcgagggtgc cgcgcaacag
     3721 ggtgatctgc gcccggacgc cccccagccc cagctcgccg gacggctgca gccacacctg
     3781 gtcggcgtgc gcggcgagca cgtagggcag caacgccgtg ccgaactcgc cgaacgactc
     3841 ggtgtaggcc cagaccggct tgtgctcacc gaaccccgcg atcgagtcgg ccagctcgct
     3901 ggcctgtgag gcgctgaccg ggcaggtacc gatgtgcacg atcaggccca cgaccgcttc
     3961 gtcggcggcg gcggcgtgca ggccgtcgcg cagtgcccgc atggtgggcg cattgatcag
     4021 cttcaacgcc tcgaacgggt tggccggggc gctgctgagc accgagaagt cgaggtcgag
     4081 ttcgaggatc acccgatcgc cgtgcgcgga cgtgcggaac agg
//
LOCUS       DAOSUG010000256         4113 bp    DNA     linear   ENV 10-JUN-2024
DEFINITION  MAG TPA_asm: Micropruina sp. isolate AMR_MDS_2195
            NODE_9130_length_4113_cov_3.482257, whole genome shotgun sequence.
ACCESSION   DAOSUG010000256 DAOSUG010000000
VERSION     DAOSUG010000256.1
DBLINK      BioProject: PRJNA925477
            BioSample: SAMN32864262
            Sequence Read Archive: SRR4236673
KEYWORDS    WGS; ENV; Metagenome Assembled Genome; MAG; Third Party Data; TPA;
            TPA:assembly.
SOURCE      Micropruina sp. (wastewater metagenome)
  ORGANISM  Micropruina sp.
            Bacteria; Bacillati; Actinomycetota; Actinomycetes;
            Propionibacteriales; Nocardioidaceae; Micropruina.
REFERENCE   1  (bases 1 to 4113)
  AUTHORS   Abdulkadir,N., Saraiva,J.P., Zhang,J., Stolte,S., Gillor,O.,
            Harms,H. and Rocha,U.
  TITLE     Genome-centric analyses of 165 metagenomes show that mobile genetic
            elements are crucial for the transmission of antimicrobial
            resistance genes to pathogens in activated sludge and wastewater
  JOURNAL   Microbiol Spectr 12 (3), e0291823 (2024)
   PUBMED   38289113
REFERENCE   2  (bases 1 to 4113)
  AUTHORS   Abdulkadir,N.
  TITLE     Direct Submission
  JOURNAL   Submitted (24-MAR-2023) Environmental Microbiology, Helmholtz
            Center for Environmental Research -UFZ, Permoserstr 15, Leipzig
            04318, Germany
COMMENT     The annotation was added by the NCBI Prokaryotic Genome Annotation
            Pipeline (PGAP). Information about PGAP can be found here:
            https://www.ncbi.nlm.nih.gov/genome/annotation_prok/
            
            ##Genome-Assembly-Data-START##
            Assembly Method        :: SPAdes v. 3.11.1
            Genome Representation  :: Full
            Expected Final Version :: Yes
            Genome Coverage        :: Not Applicable
            Sequencing Technology  :: Illumina MiSeq
            ##Genome-Assembly-Data-END##
            
            ##Genome-Annotation-Data-START##
            Annotation Provider               :: NCBI
            Annotation Date                   :: 05/04/2023 04:57:10
            Annotation Pipeline               :: NCBI Prokaryotic Genome
                                                 Annotation Pipeline (PGAP)
            Annotation Method                 :: Best-placed reference protein
                                                 set; GeneMarkS-2+
            Annotation Software revision      :: 6.5
            Features Annotated                :: Gene; CDS; rRNA; tRNA; ncRNA
            Genes (total)                     :: 2,776
            CDSs (total)                      :: 2,750
            Genes (coding)                    :: 2,714
            CDSs (with protein)               :: 2,714
            Genes (RNA)                       :: 26
            tRNAs                             :: 24
            ncRNAs                            :: 2
            Pseudo Genes (total)              :: 36
            CDSs (without protein)            :: 36
            Pseudo Genes (ambiguous residues) :: 0 of 36
            Pseudo Genes (frameshifted)       :: 2 of 36
            Pseudo Genes (incomplete)         :: 32 of 36
            Pseudo Genes (internal stop)      :: 2 of 36
            ##Genome-Annotation-Data-END##
FEATURES             Location/Qualifiers
     source          1..4113
                     /organism="Micropruina sp."
                     /mol_type="genomic DNA"
                     /submitter_seqid="NODE_9130_length_4113_cov_3.482257"
                     /isolate="AMR_MDS_2195"
                     /isolation_source="Activated sludge of municipal
                     wastewater treatment plants"
                     /db_xref="taxon:2737536"
                     /environmental_sample
                     /geo_loc_name="Canada"
                     /lat_lon="49.25 N 123.1 W"
                     /collection_date="2015-06-16"
                     /metagenome_source="wastewater metagenome"
                     /note="metagenomic"
     gene            complement(<1..532)
                     /locus_tag="PLF56_08940"
     CDS             complement(<1..532)
                     /locus_tag="PLF56_08940"
                     /inference="COORDINATES: protein motif:HMM:NF027798.2"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="beta-1,3-glucanase family protein"
                     /protein_id="HOQ53724.1"
                     /translation="MPPTITALPARRAHRTSALHSHARRLVTLLLALAVAVVTLWGNT
                     PGTATAAAGLSVTIRNTTGSSQPVYVYVLGTRLTDHVSGYVTAGGAFRAWPAVSGSAT
                     RAAPDVAIRGPRNGAATTVRLPHGISGRLYYSVGAKLDLRLVRDGTGRTGLVQPAPWV
                     AGDASASTLFDWVEFTY"
     gene            1110..3137
                     /gene="thrS"
                     /locus_tag="PLF56_08945"
                     /pseudo
     CDS             1110..3137
                     /gene="thrS"
                     /locus_tag="PLF56_08945"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_002545954.1"
                     /note="incomplete; partial in the middle of a contig;
                     missing C-terminus; Derived by automated computational
                     analysis using gene prediction method: Protein Homology.
                     GO_component: GO:0005737 - cytoplasm [Evidence IEA];
                     GO_function: GO:0004829 - threonine-tRNA ligase activity
                     [Evidence IEA];
                     GO_process: GO:0006435 - threonyl-tRNA aminoacylation
                     [Evidence IEA]"
                     /pseudo
                     /codon_start=1
                     /transl_table=11
                     /product="threonine--tRNA ligase"
     gene            3194..3760
                     /locus_tag="PLF56_08950"
     CDS             3194..3760
                     /locus_tag="PLF56_08950"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_015070367.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="HIT domain-containing protein"
                     /protein_id="HOQ53725.1"
                     /translation="MAEHPEGVIVEAAGERPGVPDAFQRLWTPHRMVYIDGENKPSDA
                     TQGQCPFCREPSRPHDESLIVHRGEAAFVVMNLYPYSPGHLLVCPYRHVSDYTELTPS
                     EVTEVAGLTQRAMTVIRSVSHPAGFNLGINQGDVAGAGIAAHLHQHVVPRWQGDMNFL
                     PVIAQTKAVPQLLGDTRDLLAHAWTASR"
     gene            3928..>4113
                     /locus_tag="PLF56_08955"
     CDS             3928..>4113
                     /locus_tag="PLF56_08955"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_014846587.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_component: GO:0016020 - membrane [Evidence IEA];
                     GO_function: GO:0016780 - phosphotransferase activity, for
                     other substituted phosphate groups [Evidence IEA];
                     GO_process: GO:0008654 - phospholipid biosynthetic process
                     [Evidence IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="CDP-alcohol phosphatidyltransferase family
                     protein"
                     /protein_id="HOQ53726.1"
                     /translation="MSSGEPYDTERILTLPNVLSFARLLGVPLFLWLLLARQYDLLAV
                     VALVFLGATDWVDGYLAR"
BASE COUNT          671 a         1398 c         1413 g          631 t
ORIGIN      
        1 ggtaggtgaa ctcgacccag tcgaacagcg tggacgcgct cgcgtcgcca gccacccagg
       61 gcgccggctg caccaggccg gtgcgacccg tgccgtcccg gacgagccgc aggtcgagtt
      121 tcgcgccgac ggagtagtag agccgtccgg agatgccgtg cggcagtcgc accgtcgtag
      181 cggcaccgtt gcgggggccg cggatggcga cgtccggtgc ggcccgggtc gccgatcccg
      241 acaccgccgg ccacgccctg aaggcgcccc ccgcggtcac gtagccggac acgtgatcgg
      301 tgaggcgagt acccaacacg tacacgtaca ccggctggga cgagccggtc gtgttgcgta
      361 tggtgacgct cagtccggcg gccgcggtcg cggtgccagg ggtgttaccc cacagcgtga
      421 cgacggcgac ggccagtgcc aacagcaggg tgaccaagcg gcgcgcgtga ctgtgcaggg
      481 cagaggtgcg gtgggcccgg cgggcaggta gtgcggtgat ggtggggggc atggtgtgct
      541 cctttcgggg tgtgcaacac cggtgccatg aaccatgcat agtcaaggtg acgatgtggt
      601 gacacgccca gtttcgcgag aacgccttga ctttttttga cgtaagcgac aatatgaccg
      661 cgcgttcgac ctacacgcga gcggcgactc gtcccgtacc ccgcatcgtc aggccgacgc
      721 cgcgcgatcg gcgcggcagg gattgatgcg atcgccgtcc caagtcaccc atgcagggga
      781 caagacgggc tggcagagcc actgcggccc gtctagagtt tcttgcgggg gacaacccat
      841 cctggcgggg ggatctgcag ggtgaggttg ccaaagggga aacggccgta ggcccgacat
      901 cctgacatga gcgaggccgg gttccgaccg gggggatcgg aatccggcct cgttttgtct
      961 gcccagaggt gtccggtgag gcgtatccgc tccaacggcc tgctcgaacg tgttgatcgt
     1021 cagtcccgcg cagcgccgcg cagcggaagg gttgatcggt ttcacctgcc gctgaccgcg
     1081 cccacacgct cagcaccgat tgcggcacaa tggacgctgc gcccggctcc gggcgcccga
     1141 ttcccggtcc gaaaggaagt tctgtcgtgt ctgtctccat caccgtggtg cgccccgatt
     1201 cgcgcgaaac ccgggtggtc gagactgcga ccaccggcct cgacctgttc ggggccgacc
     1261 gcaccgtggt cgccatgcgg gtgggcggcg agctgcgtga cctgtccggc gaactggccg
     1321 cggacgacgt ggtcgagccg gtcggcatcg acagccccga cgggctgaac attctgcggc
     1381 actccaccgg gcacgtcacc gcccaggcgg tgcaggccct gtaccccgac accaagctcg
     1441 gcatcggccc gttcatcgcc gacggctact actacgactt cgacgcggcg cgtccgttca
     1501 cccccgacga cgtggcggcg atcgagaagc agatggggca gatcgtccgt gagcggcaga
     1561 agttcgtccg ccgggtggtc agcgacgccg aagcgcgcgc cgaactggcg tccgagccgt
     1621 acaagctcga actgatcggc atcaagggca acaccgaggg caccgagggt gccggcgtcg
     1681 aggtgggcgg cggagaactg accatctacg acaacgtgcg acgcgacggc agcgtggcgt
     1741 ggaaggacct ctgccgcggc ccgcacgtgc cgcacaccgg gtacctcaat gcgtactcgc
     1801 tgatgcgcac gtccgcggcc tactggcgcg gcgaccagcg caacgcgcaa ctgcagcgca
     1861 tctacggcac ctcctggccc agccgcgacg acctgaaggc gtacaagttt cggctggacg
     1921 aggccgcgaa gcgcgaccat cgcaagctgg gcgccgagct cgacctgttc agcttcccgg
     1981 acgagatcgg ctccggcctg cccgtgttcc accccaaggg cgcgatcgtg cgcatggaga
     2041 tggaggacta cagccggcgc cggcacgtcg aggagggcta tgagttcgcc tacagcccac
     2101 acctgaccaa ggcgcagttg ttcgagacct ccggccacct cgactggtac gccgacggca
     2161 tgtacccgcc catgcacctc gacgaggagc gtgacgccga cggcaacgtc cggcgccagg
     2221 ggcaggacta ctacatgaag ccgatgaact gcccgatgca ctgcctgatc taccaatcgc
     2281 gcggacggtc ctaccgcgaa ctgccgctgc ggctgttcga gttcggcacc gtgtaccgct
     2341 acgagaagtc gggggtgatc cacgggctga cccgggcccg cggcttcacc caggacgacg
     2401 cgcacatcta tgccaccaag gagcagctgg gcgaggagtt ggagcggctg ctcacctttg
     2461 tgctgaagct gctcgccgac tacggcctgg aggacttcta cctggagctc tcgacccgca
     2521 accccgacaa gtcgatcggc gacgagcaga cctggcaggt ggccaccgac accctgcgcg
     2581 aggtggccga gcgctccggt ctggagttgg tgcccgatcc gggcggcgca gccttctacg
     2641 gcccgaagat ctcggtgcag gcccgcgacg cgatcggccg cacctggcag atgtcgacca
     2701 ttcagctcga cttcttcgag cccgagcggt tcgagctcga atacaccgcg cccgatggca
     2761 gcaggcagcg tccggtgatg atccaccggg ccctgttcgg atcgatcgaa cggttcttcg
     2821 gcgtgctcac cgagcactac gcgggcgcgt tcccggcctg gctggcccct gtccaggtga
     2881 tcggcctacc ggtcgcggac gagttcaacg agtacctcgc gggggtgctg gatcaactgc
     2941 gcgcgcaggg cgtccgggtc gaactggacg cctcggacga ccggttcggc aagaagatcc
     3001 gcaacgcgtc caagagcaag gcgccgttcg tgctgatcgc cggtggcgac gaccgtgacg
     3061 cgggagcggt gagcttccgc taccgggacg ggtcgcagcg aaacggcgtg ccgatcgcgc
     3121 aggccgtcca ggtgatcgtc gagcacatcg cgagccgatc caacgccgac ccgaccgccg
     3181 agggctggtc cgcatggccg agcaccccga gggcgtcatc gtcgaggcgg ccggcgagcg
     3241 ccccggggtg ccggacgcct ttcaacggct ctggacgccg caccgcatgg tctacatcga
     3301 cggcgagaac aagccgtccg atgcgaccca ggggcagtgc ccgttctgcc gggagccgtc
     3361 gcggccgcac gatgagagtc tgatcgtgca ccgcggtgag gcggccttcg tggtgatgaa
     3421 cctctacccc tattcacccg ggcacctgtt ggtgtgcccc taccggcacg tgtcggacta
     3481 caccgagctc accccgtccg aggtgaccga ggtggccggc ctcacccaac gtgccatgac
     3541 ggtgatccgt tcggtcagcc acccggcggg gttcaatctg ggcatcaacc agggcgacgt
     3601 ggcgggcgcc ggcatcgccg cccacctgca ccagcacgtg gtgccgcgct ggcagggcga
     3661 catgaacttt ctgccggtga tcgcccagac caaggcggtg ccgcaactgc tcggtgacac
     3721 ccgcgacctg ctggcgcacg cgtggaccgc ctcacgttga acagaacctg ggcgacgggt
     3781 cctttcgtac tcacggtcgt ccagccggcc cgatgcgtcg tctcgcgccc tcgtcgagcg
     3841 tggctcgatc gggcgcgagc ggcaggtcga tgccggtcga cgtcgtccgc gtccggctcg
     3901 gtgggggagg gccactaggc tgaccccgtg tcatccggcg agccctacga caccgagcgg
     3961 attctcaccc tccccaatgt gctgagcttc gcccggctgc tcggcgtgcc cctgttcctg
     4021 tggttgctgc tcgcccgcca gtacgacctg ctcgccgtgg tggcactggt ctttctcggt
     4081 gccaccgact gggtggacgg atacctggcc cgc
//
LOCUS       DAOSUG010000257         4108 bp    DNA     linear   ENV 10-JUN-2024
DEFINITION  MAG TPA_asm: Micropruina sp. isolate AMR_MDS_2195
            NODE_9141_length_4108_cov_4.137923, whole genome shotgun sequence.
ACCESSION   DAOSUG010000257 DAOSUG010000000
VERSION     DAOSUG010000257.1
DBLINK      BioProject: PRJNA925477
            BioSample: SAMN32864262
            Sequence Read Archive: SRR4236673
KEYWORDS    WGS; ENV; Metagenome Assembled Genome; MAG; Third Party Data; TPA;
            TPA:assembly.
SOURCE      Micropruina sp. (wastewater metagenome)
  ORGANISM  Micropruina sp.
            Bacteria; Bacillati; Actinomycetota; Actinomycetes;
            Propionibacteriales; Nocardioidaceae; Micropruina.
REFERENCE   1  (bases 1 to 4108)
  AUTHORS   Abdulkadir,N., Saraiva,J.P., Zhang,J., Stolte,S., Gillor,O.,
            Harms,H. and Rocha,U.
  TITLE     Genome-centric analyses of 165 metagenomes show that mobile genetic
            elements are crucial for the transmission of antimicrobial
            resistance genes to pathogens in activated sludge and wastewater
  JOURNAL   Microbiol Spectr 12 (3), e0291823 (2024)
   PUBMED   38289113
REFERENCE   2  (bases 1 to 4108)
  AUTHORS   Abdulkadir,N.
  TITLE     Direct Submission
  JOURNAL   Submitted (24-MAR-2023) Environmental Microbiology, Helmholtz
            Center for Environmental Research -UFZ, Permoserstr 15, Leipzig
            04318, Germany
COMMENT     The annotation was added by the NCBI Prokaryotic Genome Annotation
            Pipeline (PGAP). Information about PGAP can be found here:
            https://www.ncbi.nlm.nih.gov/genome/annotation_prok/
            
            ##Genome-Assembly-Data-START##
            Assembly Method        :: SPAdes v. 3.11.1
            Genome Representation  :: Full
            Expected Final Version :: Yes
            Genome Coverage        :: Not Applicable
            Sequencing Technology  :: Illumina MiSeq
            ##Genome-Assembly-Data-END##
            
            ##Genome-Annotation-Data-START##
            Annotation Provider               :: NCBI
            Annotation Date                   :: 05/04/2023 04:57:10
            Annotation Pipeline               :: NCBI Prokaryotic Genome
                                                 Annotation Pipeline (PGAP)
            Annotation Method                 :: Best-placed reference protein
                                                 set; GeneMarkS-2+
            Annotation Software revision      :: 6.5
            Features Annotated                :: Gene; CDS; rRNA; tRNA; ncRNA
            Genes (total)                     :: 2,776
            CDSs (total)                      :: 2,750
            Genes (coding)                    :: 2,714
            CDSs (with protein)               :: 2,714
            Genes (RNA)                       :: 26
            tRNAs                             :: 24
            ncRNAs                            :: 2
            Pseudo Genes (total)              :: 36
            CDSs (without protein)            :: 36
            Pseudo Genes (ambiguous residues) :: 0 of 36
            Pseudo Genes (frameshifted)       :: 2 of 36
            Pseudo Genes (incomplete)         :: 32 of 36
            Pseudo Genes (internal stop)      :: 2 of 36
            ##Genome-Annotation-Data-END##
FEATURES             Location/Qualifiers
     source          1..4108
                     /organism="Micropruina sp."
                     /mol_type="genomic DNA"
                     /submitter_seqid="NODE_9141_length_4108_cov_4.137923"
                     /isolate="AMR_MDS_2195"
                     /isolation_source="Activated sludge of municipal
                     wastewater treatment plants"
                     /db_xref="taxon:2737536"
                     /environmental_sample
                     /geo_loc_name="Canada"
                     /lat_lon="49.25 N 123.1 W"
                     /collection_date="2015-06-16"
                     /metagenome_source="wastewater metagenome"
                     /note="metagenomic"
     gene            complement(<1..186)
                     /locus_tag="PLF56_08960"
     CDS             complement(<1..186)
                     /locus_tag="PLF56_08960"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_020574454.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="NAD-dependent succinate-semialdehyde
                     dehydrogenase"
                     /protein_id="HOQ53727.1"
                     /translation="MSSNREAELLAGVPDGLFIGGEWRAASGGGTLAVRDPSDGSVIR
                     QIADGTVDDGLAALDAAV"
     gene            complement(353..703)
                     /locus_tag="PLF56_08965"
     CDS             complement(353..703)
                     /locus_tag="PLF56_08965"
                     /inference="COORDINATES: ab initio
                     prediction:GeneMarkS-2+"
                     /note="Derived by automated computational analysis using
                     gene prediction method: GeneMarkS-2+."
                     /codon_start=1
                     /transl_table=11
                     /product="hypothetical protein"
                     /protein_id="HOQ53728.1"
                     /translation="MFLGDVIGQPVLADGQRVGYLSDVRLFVPDHTAGQQVGTPEVYG
                     VVICPRRTGSFLGFERRGVRAPWLLASWYRWRARGSFLVLWADVAAWGDGGVDLRPDA
                     PRWSPEVGGGSAPP"
     gene            complement(690..1076)
                     /locus_tag="PLF56_08970"
     CDS             complement(690..1076)
                     /locus_tag="PLF56_08970"
                     /inference="COORDINATES: ab initio
                     prediction:GeneMarkS-2+"
                     /note="Derived by automated computational analysis using
                     gene prediction method: GeneMarkS-2+."
                     /codon_start=1
                     /transl_table=11
                     /product="hypothetical protein"
                     /protein_id="HOQ53729.1"
                     /translation="MTLPRPPRPGGGRLIDARLSLLDRQVLDASGQPVGVVDDLEVSE
                     LPEPTGDGRVRVLNLVHGPIVLERIVGGRRPKAHFERVPWSTVAKLGSAIELAVDADE
                     VPVGWLEDWLARNVIARIPGGRHVPR"
     gene            complement(1073..2299)
                     /locus_tag="PLF56_08975"
     CDS             complement(1073..2299)
                     /locus_tag="PLF56_08975"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_011755333.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_component: GO:0016020 - membrane [Evidence IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="divalent metal cation transporter"
                     /protein_id="HOQ53730.1"
                     /translation="MKRLFAVALGILTAIGGFLDIGDIVTNAVVGSRFGMSLAWVVLV
                     GVAGICVFAHMAGKVAAVSGRATFEVIRERLGPRMALANLGASFLINLLTLTAEVGGI
                     ALALQLASSVHYLLWVPVAALAVWLVIWRVGFEKMENVTGLVGLLLIGFAVAFFLLGP
                     DWGELGAQVLQRLPGNEASATYWFYAVALFGAALTPYEVFFFSSGAVEEGWTVKDLGQ
                     SRLNVLVGFPLGGLLSLAIAGCAALVLLPRGIEVTSLGQVLLPVAEAGGKLLLVAVIL
                     GVVAATFGAALETTLSAGYTVAQFFGWTWGKFRRPAQAARFHVTMMICMIIGLGVLAT
                     GVDPVMVTEISVVFSAAALPLTYLPILIVSNDPDYMGEHVNGRALNVAGQVYFVLILV
                     ASLAAIPLLIVTGVGQ"
     gene            complement(2310..2717)
                     /locus_tag="PLF56_08980"
     CDS             complement(2310..2717)
                     /locus_tag="PLF56_08980"
                     /inference="COORDINATES: ab initio
                     prediction:GeneMarkS-2+"
                     /note="Derived by automated computational analysis using
                     gene prediction method: GeneMarkS-2+."
                     /codon_start=1
                     /transl_table=11
                     /product="hypothetical protein"
                     /protein_id="HOQ53731.1"
                     /translation="MRLGWVALVASVLLTGCSGGPATDPAALAVSESHSAVAGLALAV
                     RLRLENRSTDAYTQVLLDTTRDAVADAQRELVVAADADPARRAPVAALVTRAASELSA
                     LAQAGAAELDADDLSRLEALERQLRDLATQLGR"
     gene            2974..>4108
                     /locus_tag="PLF56_08985"
     CDS             2974..>4108
                     /locus_tag="PLF56_08985"
                     /inference="COORDINATES: protein motif:HMM:NF015746.2"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_function: GO:0015558 - secondary active
                     p-aminobenzoyl-glutamate transmembrane transporter
                     activity [Evidence IEA];
                     GO_process: GO:1902604 - p-aminobenzoyl-glutamate
                     transmembrane transport [Evidence IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="AbgT family transporter"
                     /protein_id="HOQ53732.1"
                     /translation="MSDTEKTQKKKESGGTFLDTVERLGNKVPNPTIMFLYLIAVIAV
                     LSAILSWAGVSVTDEVVTPVPKDEFSQINEHLGGTWSIYDSTTGEPAEVPDFIVSEQT
                     FPVRNLISVEGARHFFSSFVDNFAGFGVVAVVLISMAGVGVAEHAGMMGALIRKVVKI
                     APPRWLTFILVFVGVLSSVATDAGYLILVPLAAAALMSVGRHPLAGLAAAFAGVGAVF
                     AVNLLITPSDSMLTEITNEVLAKANMPALEVTQNYYFAVVSSFVMAIVVTLVTINITQ
                     KRLGVYNQADLVMGDQADEVVDEVAEAKGLKYAGWFLLGYVLLIALLTAPPGAPLRDP
                     LTGNIIGTTPFMASLVFIISLAFLVCGMGYGLGAGTLKGGNEAV"
BASE COUNT          700 a         1490 c         1318 g          600 t
ORIGIN      
        1 gacggcagcg tccagggcgg ccagcccgtc gtcgacggta ccgtccgcga tctgccggat
       61 caccgaccca tcggacggat cccgcaccgc gagcgtgccg ccaccggagg ccgcccgcca
      121 ctcacccccg atgaacaggc catcgggaac accggccagc agttcggctt cacggttgct
      181 actcatcgga cgtccttgtc atgaccagtc tgcgaggcga tacccctcca caatgctggc
      241 cgcgttcgtg gcggtcaagg ccgcacgggc ctcgggcatg ggcgacctgg tggtcgcgcc
      301 gagcgccccc ctgactatct ctggcctgcg aaggtccgca ggccctggaa agttagggag
      361 gcgctgaccc cccacctacc tcgggactcc accgcggggc gtccggacgc aggtcgacgc
      421 ccccgtcgcc ccaggccgcc acgtcggccc acagaaccaa gaaggacccg cgcgcccgcc
      481 agcggtacca ggaggccagc agccagggcg cccggacgcc gcggcgctcg aaccccagaa
      541 acgacccggt gcgccgagga cagatcacca ccccgtacac ctcgggtgtg ccgacctgct
      601 ggccggcggt gtggtccggc acgaacaacc ggacgtcgct gaggtacccg acccgctgcc
      661 cgtccgcgag cacgggctgt ccgatcacgt caccgaggaa catgacgacc ccccggaatg
      721 cgagcgatca cgttgcgggc cagccagtcc tccagccaac cgaccggcac ctcgtcggcg
      781 tccacggcca gttcgatcgc cgagccgagc ttcgccaccg tcgaccacgg cacccgttcg
      841 aagtgcgcct tcggacggcg cccgccgacg attcgctcca gcacgatggg gccgtgcacc
      901 aggttgagca cccggacccg gccatcaccg gtcggctcgg gcagctcgct gacctcgagg
      961 tcgtccacca ccccgacggg ttggccggag gcgtccagca cctgccggtc gagcaggctg
     1021 agccgcgcat cgatgagacg tccgcctccg ggacgcggcg gtctcggcaa cgtcattggc
     1081 ccacccccgt gacgatcagc agcggaatcg cggccagcga tgcgaccaga atcagcacga
     1141 agtacacctg gccggccacg ttcagcgccc gtccgttgac gtgctcgccc atgtagtccg
     1201 gatcgttgct gacgatcagg atcggcaggt aggtgagcgg cagcgccgcc gccgagaaca
     1261 ccaccgagat ctcggtcacc atcaccgggt cgacgccggt ggccagcacc cccaggccga
     1321 tgatcatgca gatcatcatc gtgacgtgaa agcgggccgc ctgggctggc cggcggaact
     1381 tgccccacgt ccagccgaag aactgggcca ccgtgtagcc cgcagacagg gtggtttcga
     1441 gggcagcgcc gaacgtggcg gccaccacac cgaggatcac cgctaccagc agcagcttgc
     1501 cccccgcctc ggcgaccggc agcagcacct ggcccagcga ggtcacctcg atgccccgcg
     1561 gcaacagcac caacgccgcg caacctgcga tcgccagcga cagcagtccg ccgagcggga
     1621 atccgaccag cacgttcagc cgcgactggc cgagatcctt caccgtccag ccttcttcga
     1681 ccgccccgga gctgaagaag aacacctcat acggggtcag tgcggcgccg aacagcgcca
     1741 cggcatagaa ccagtaggtc gcgctcgcct cgttgccggg caaccgctgc agcacctggg
     1801 cgcccagttc gccccagtcg ggcccgagca gaaagaacgc aacggcgaag ccgatcagca
     1861 acaggccgac caggccggtg acgttctcca tcttctcgaa gccgacccgc cagatcacca
     1921 gccacaccgc gagcgccgcg accggcaccc acagcaggta gtgcacgctg ctggccagtt
     1981 gcagggccaa cgcgatgccg cccacttcgg cggtcagggt gagcaggttg atcagaaagg
     2041 acgcccccag gttggccagg gccatccgcg gtccgagccg ttcgcggatc acctcgaagg
     2101 tggcccgtcc gctgaccgcc gcgaccttgc cggccatgtg ggcgaacacg cagatgccgg
     2161 ccacgccgac cagcaccacc cacgccagcg acatgccgaa ccgcgacccg accaccgcgt
     2221 tggtgacgat gtcgccgatg tcgaggaagc cgccgatggc ggtgagaatg cccagggcga
     2281 ccgcgaagag tcgtttcatc ggcgcgtccc tatcggccca gctgcgtggc gagatcgcgc
     2341 agttgccgtt cgagagcctc caagcgcgac aggtcgtccg cgtccagctc ggcggcaccc
     2401 gcctgcgcca gcgcgctcag ttcggacgcc gcccgcgtca cgagcgcggc gaccggagcc
     2461 ctgcgggcgg ggtccgcgtc cgctgcgacc accaactcgc gctgggcgtc cgcgaccgca
     2521 tcgcgggtgg tgtcgagcag cacctgggtg taggcgtccg tgctgcgatt ctcaagccgc
     2581 agccgcaccg cgagtgcgag gccggccacc gcggagtgcg actcgctcac ggcgagagcg
     2641 gcgggatcgg tggccggacc gcccgagcag ccggtcagca gcacgctggc caccagcgcc
     2701 acccagccga gtctcatgcg cacctcccag cgctaacgat ggccaccctc gacgttgaca
     2761 cgcctcaccc ctgggggcgt acagaccgcg ccttttcgcg gattcctccc ctagttccag
     2821 gggtcgctcg gccgctcggg ccgtcccatt tcgcggtctt tcgagaaacc cgtgagaatg
     2881 cctccccgca gccggccaat ccgctggata ctacgggcaa caccccggcg cggagtgggt
     2941 acacgctggc tgtttccccg agcgaggcgc accatgtctg atacagagaa aacacagaag
     3001 aagaaggaat ccggcggcac gttcctcgac acggtcgaac gtcttggtaa caaggtgccc
     3061 aatcccacga tcatgttcct ctacctgatc gcggtgatcg cagtactgtc agcgatcctg
     3121 tcgtgggcgg gggtgagcgt caccgacgag gtggtcacgc cggttcccaa agacgagttc
     3181 agccagatca acgagcatct cggcggcacc tggtcgatct acgactcgac caccggcgag
     3241 ccggccgagg tgcccgactt catcgtgtcc gagcagacct ttccggtgcg caacctgatc
     3301 tcggtcgagg gcgcgcggca cttcttctcg tccttcgtcg acaacttcgc cggtttcggc
     3361 gtggtggcgg tggtgctgat ctcgatggcc ggtgtcggcg tcgccgagca tgccggcatg
     3421 atgggcgctc tgatccgcaa ggtggtgaag atcgccccac cccgctggtt gaccttcatt
     3481 ctggtcttcg tcggcgtgct gtcgtcggtg gccaccgatg ccggctacct gattctggtg
     3541 ccgctcgccg cggccgcctt gatgtctgtc ggacgccacc cgctggccgg tctcgcggcg
     3601 gccttcgccg gggtcggtgc agtgttcgcc gtgaacctgc tgatcacccc gtccgacagc
     3661 atgctcaccg agatcaccaa cgaggtgctg gccaaggcca acatgccggc gctcgaagtg
     3721 acgcagaact actacttcgc ggtggtctcg tcgttcgtca tggcgatcgt ggtgacgttg
     3781 gtcacgatca acatcaccca gaagcggctg ggcgtctaca accaggccga cctggtgatg
     3841 ggcgaccagg cagacgaggt cgtcgacgag gtggccgagg ccaagggact gaagtacgcc
     3901 ggctggttcc tgctcggcta cgtgctgctg atcgccctgc tgaccgctcc ccccggagcg
     3961 ccgctgcgcg acccgctgac cggcaacatc atcggcacca cgccgttcat ggcgagcctg
     4021 gtgttcatca tctcgctggc gtttctggtc tgcggcatgg gctacggcct gggcgcgggc
     4081 acgctcaagg gcggcaatga ggccgttg
//
LOCUS       DAOSUG010000258         4105 bp    DNA     linear   ENV 10-JUN-2024
DEFINITION  MAG TPA_asm: Micropruina sp. isolate AMR_MDS_2195
            NODE_9145_length_4105_cov_3.386173, whole genome shotgun sequence.
ACCESSION   DAOSUG010000258 DAOSUG010000000
VERSION     DAOSUG010000258.1
DBLINK      BioProject: PRJNA925477
            BioSample: SAMN32864262
            Sequence Read Archive: SRR4236673
KEYWORDS    WGS; ENV; Metagenome Assembled Genome; MAG; Third Party Data; TPA;
            TPA:assembly.
SOURCE      Micropruina sp. (wastewater metagenome)
  ORGANISM  Micropruina sp.
            Bacteria; Bacillati; Actinomycetota; Actinomycetes;
            Propionibacteriales; Nocardioidaceae; Micropruina.
REFERENCE   1  (bases 1 to 4105)
  AUTHORS   Abdulkadir,N., Saraiva,J.P., Zhang,J., Stolte,S., Gillor,O.,
            Harms,H. and Rocha,U.
  TITLE     Genome-centric analyses of 165 metagenomes show that mobile genetic
            elements are crucial for the transmission of antimicrobial
            resistance genes to pathogens in activated sludge and wastewater
  JOURNAL   Microbiol Spectr 12 (3), e0291823 (2024)
   PUBMED   38289113
REFERENCE   2  (bases 1 to 4105)
  AUTHORS   Abdulkadir,N.
  TITLE     Direct Submission
  JOURNAL   Submitted (24-MAR-2023) Environmental Microbiology, Helmholtz
            Center for Environmental Research -UFZ, Permoserstr 15, Leipzig
            04318, Germany
COMMENT     The annotation was added by the NCBI Prokaryotic Genome Annotation
            Pipeline (PGAP). Information about PGAP can be found here:
            https://www.ncbi.nlm.nih.gov/genome/annotation_prok/
            
            ##Genome-Assembly-Data-START##
            Assembly Method        :: SPAdes v. 3.11.1
            Genome Representation  :: Full
            Expected Final Version :: Yes
            Genome Coverage        :: Not Applicable
            Sequencing Technology  :: Illumina MiSeq
            ##Genome-Assembly-Data-END##
            
            ##Genome-Annotation-Data-START##
            Annotation Provider               :: NCBI
            Annotation Date                   :: 05/04/2023 04:57:10
            Annotation Pipeline               :: NCBI Prokaryotic Genome
                                                 Annotation Pipeline (PGAP)
            Annotation Method                 :: Best-placed reference protein
                                                 set; GeneMarkS-2+
            Annotation Software revision      :: 6.5
            Features Annotated                :: Gene; CDS; rRNA; tRNA; ncRNA
            Genes (total)                     :: 2,776
            CDSs (total)                      :: 2,750
            Genes (coding)                    :: 2,714
            CDSs (with protein)               :: 2,714
            Genes (RNA)                       :: 26
            tRNAs                             :: 24
            ncRNAs                            :: 2
            Pseudo Genes (total)              :: 36
            CDSs (without protein)            :: 36
            Pseudo Genes (ambiguous residues) :: 0 of 36
            Pseudo Genes (frameshifted)       :: 2 of 36
            Pseudo Genes (incomplete)         :: 32 of 36
            Pseudo Genes (internal stop)      :: 2 of 36
            ##Genome-Annotation-Data-END##
FEATURES             Location/Qualifiers
     source          1..4105
                     /organism="Micropruina sp."
                     /mol_type="genomic DNA"
                     /submitter_seqid="NODE_9145_length_4105_cov_3.386173"
                     /isolate="AMR_MDS_2195"
                     /isolation_source="Activated sludge of municipal
                     wastewater treatment plants"
                     /db_xref="taxon:2737536"
                     /environmental_sample
                     /geo_loc_name="Canada"
                     /lat_lon="49.25 N 123.1 W"
                     /collection_date="2015-06-16"
                     /metagenome_source="wastewater metagenome"
                     /note="metagenomic"
     gene            complement(<1..1310)
                     /gene="crtI"
                     /locus_tag="PLF56_08990"
     CDS             complement(<1..1310)
                     /gene="crtI"
                     /locus_tag="PLF56_08990"
                     /EC_number="1.-.-.-"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_013862672.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_function: GO:0016166 - phytoene dehydrogenase activity
                     [Evidence IEA];
                     GO_process: GO:0016117 - carotenoid biosynthetic process
                     [Evidence IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="phytoene desaturase family protein"
                     /protein_id="HOQ53733.1"
                     /translation="MSRVVVVGAGLSGLSAACHLVADGHDVTVLERDATPGGRNGVFR
                     SEGFTFDTGPTVLTMPGLLERPLHRIGGSLDPLRLRRLDPGYRAFFADGSRIDVRASV
                     AAMTRELAEQVSPKDAAAFENFVGWLEELYDVELPNFIERNFDSPLDLLTRPRAAARL
                     VRLGGFGRLGPAIRRRFDDDRLVRLFTFQAMYAGLAPEDALALYAVSTYMDSVSGVWF
                     PADGMHAVPTTMAGALEAAGARLHYRTPAERILTDARGRVAGVQAGGQKLVADAVVLT
                     ADLPAAYRTLLPDLRPPRAVRTGSYSPSAVVWHLGVRGTVPGHVSHHNIHFADAWGEA
                     FGQLIKRKELMSDPSRLVTIPTLDAPGMAPAGHSVLYVLEPVPNLTADLDWAVEGPRL
                     RERLLGFLQAGGYPTDIVTESLVTPADWTAQGMAAGTPFSLAHS"
     gene            complement(1307..2452)
                     /locus_tag="PLF56_08995"
     CDS             complement(1307..2452)
                     /locus_tag="PLF56_08995"
                     /inference="COORDINATES: protein motif:HMM:NF012568.2"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="polyprenyl synthetase family protein"
                     /protein_id="HOQ53734.1"
                     /translation="MTASPPHQAMTTSPLELVPASQPEVAERHTVNAVLPLVEATLDE
                     LSRQWEVLASAVVPRPGVLHTDSILSLVRGFAATTGKLLRPRMAHWGWVAAGGLDHGR
                     GHDDLIRIGAALELLHCFALVHDDVMDASDTRRGNPSVHEVARAEHRELGGLGDPQRY
                     AENIAILVSDLLHSEADLLVGGLPEPLRAAWRTMAIELMMGQGRDLVGAANGRRDLQH
                     AQEVARAKSGAYTVWRPLQLGALAAGAGPDLLAVLHEYGHHAGQAFALRDDILGVIGD
                     PARTGKPVGDDLLAGKPTVLLALAEQRFGPTWRLELRRIGRDAVTAGEVARLCTELEH
                     SGVIREVEQLITTSVLAAERALSSPLVDRRAGEGLRRLVGQLAWRDS"
     gene            complement(2449..2991)
                     /gene="idi"
                     /locus_tag="PLF56_09000"
     CDS             complement(2449..2991)
                     /gene="idi"
                     /locus_tag="PLF56_09000"
                     /EC_number="5.3.3.2"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_020390560.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_function: GO:0004452 - isopentenyl-diphosphate
                     delta-isomerase activity [Evidence IEA];
                     GO_process: GO:0008299 - isoprenoid biosynthetic process
                     [Evidence IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="isopentenyl-diphosphate Delta-isomerase"
                     /protein_id="HOQ53735.1"
                     /translation="MADQVVLLDDAGRPSGAADRVSVHTGDTPYHLAFSSYLFDTAGR
                     VLLTRRALGKATWPGVWTNSCCGHPRPGESPRDAVVRRVGDELGQVPTDLRLALASFS
                     YRAVDAGGTVENELCPVWVGRVDPAGLAPDPTEVMDVAWVAWPTLVRTVAEAPQLLSP
                     WAVLQVPLLASVLAAEEGAA"
     gene            complement(2984..3799)
                     /locus_tag="PLF56_09005"
     CDS             complement(2984..3799)
                     /locus_tag="PLF56_09005"
                     /inference="COORDINATES: protein motif:HMM:NF015060.2"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="tryptophan-rich sensory protein"
                     /protein_id="HOQ53736.1"
                     /translation="MTSDPILRASLVTASAVVMVIGTLAGFGLLGTRVEESSGGALSS
                     QATLVAPANTAFSIWSLIYLGLIAYVVWQWWARGNARAARISWLAALSMLLNAAWLGV
                     TQVGLLWLSVVVILTLAVVLGLLVQRLGQSPPASRVEAVVVDGTFGVYLGWVSVATVA
                     NITAVLVDAGVDPGGIWSEALAVLVLAVAALLGAVYAFRLDGRWAIAAAMTWAVGWIA
                     AGRLGGPLYSTATAVAAIAAGIAILAATAHARSRRTARPADIAPLATTSAGRG"
BASE COUNT          630 a         1502 c         1443 g          530 t
ORIGIN      
        1 aagctgtggg cgaggctgaa cggcgtcccg gcagccattc cctgggcggt ccagtcggcc
       61 ggggtgacca gcgactcggt gacgatgtcg gtcgggtagc cgccggcctg cagaaagccc
      121 agcagccgct cccgcagcct cgggccctcg accgcccagt ccaggtcggc ggtcaggttg
      181 ggcaccggtt ccagcacgta cagcacgctg tgcccggccg gcgccatccc aggcgcgtcg
      241 agagtcggaa tggtgaccag ccgggacggg tcgctcatca gttccttgcg cttgatcagc
      301 tgcccgaagg cctctcccca ggcgtcggcg aagtggatgt tgtggtgcga gacgtgcccg
      361 gggaccgtgc cgcgcacccc caggtgccac accaccgccg agggcgagta gctccccgtc
      421 cgcaccgccc ggggtggccg caggtcgggc agcaaggtgc ggtaggccgc gggcaggtcg
      481 gcggtcagca ccaccgcgtc cgcgaccagc ttctgcccac cggcctggac gccggccacc
      541 cggccccgcg cgtctgtgag gatccgctcg gccggggtgc ggtagtgcag ccgggcgccg
      601 gcggcttcga gcgcgccggc catggtggtg ggcacggcgt gcatcccgtc ggccgggaac
      661 cagaccccgc tgaccgaatc catgtaggtg ctcaccgcgt acagtgccag cgcgtcctcg
      721 ggggcgagtc cggcgtacat cgcctggaag gtgaacagcc ggaccagccg gtcgtcgtcg
      781 aacctgcgcc gaatcgccgg tccgagacga ccgaagccgc ccagccgcac cagccgcgcg
      841 gccgcgcggg gccgggtcag caggtcgagc gggctgtcga agttgcgctc gatgaagttc
      901 ggcagttcga cgtcgtagag ctcctccagc cagccgacga aattctcgaa cgcggccgcg
      961 tccttcggcg agacctgttc ggcgagctcg cgggtcatcg ccgccaccga ggcccggacg
     1021 tcgatccggg agccgtcggc gaagaaggct cggtagccgg ggtccagccg acgcagtctc
     1081 agcggatcga ggctgccccc gatccggtgg agcggacgtt ccagcaggcc gggcatggtc
     1141 agcacggtcg gcccggtgtc gaaggtgaac ccttcggacc ggaagacacc gttgcggccg
     1201 ccgggggtgg cgtcccgctc gagcaccgtg acgtcgtggc cgtcggcaac caggtggcac
     1261 gctgcgctca gcccggacaa gccggcgccg accaccacca cccggctcat gagtcccgcc
     1321 aggccagctg cccgaccagc cggcggagcc cctcgccggc acgccggtcg accagcggtg
     1381 acgacagcgc gcgttcggcg gccagcaccg aggtggtgat cagctgctcg acctcgcgga
     1441 tcacgccgga gtgctccagt tcggtgcaga gccgggcgac ctccccggca gtgaccgcgt
     1501 cccgtccgat ccggcgcagt tccagccgcc aggtcggccc gaaccgctgc tcggcaagcg
     1561 cgagcaggac ggtgggcttg cccgcgagca gatcgtcgcc gaccggtttg ccggtgcggg
     1621 ccgggtcgcc gatcaccccg agaatgtcgt cgcgcagcgc gaaggcctgt ccggcgtgat
     1681 gcccgtactc gtgcagcacg gcgagcagat ccgggccggc accggcggcc agcgcgccga
     1741 gttgcagcgg acgccacacc gtgtaggcac cggacttggc cctggccacc tcctgggcgt
     1801 gctgcagatc gcggcggccg ttggcggccc cgaccaggtc gcgaccctgg cccatcatca
     1861 gttcgatcgc catcgtgcgc caggccgccc gcaacggctc cggcaggccg ccgaccagca
     1921 ggtcggcctc gctgtgcagc aggtcgctga ccaggatggc gatgttctcg gcgtagcgtt
     1981 gcggatcgcc gagcccgccg agttcgcggt gctcggcgcg cgccacctcg tgcaccgacg
     2041 ggttgccccg gcgggtgtcg gaggcgtcca tcacgtcgtc atggaccagc gcgaagcagt
     2101 gcagcagttc cagcgcggcg ccgatccgga tcaggtcgtc gtgcccgcgg ccgtggtcca
     2161 ggccgccggc ggcgacccag ccccagtgcg ccatccgggg acgcagcagc ttgccggtgg
     2221 tggctgcgaa gccgcgcacc agggagagga tcgagtcggt gtgcagcact cccggccggg
     2281 ggacgacagc gctcgccaac acctcccact gccggctgag ttcgtccaag gtggcctcga
     2341 ccagcggcag gaccgcgttc accgtgtggc gctcggccac ctccggctgg gatgccggta
     2401 cgagctccag cggactggtg gtcatcgcct ggtggggcgg ggatgcggtc acgctgctcc
     2461 ctcctcggct gccaggacgg aggccagcag cggcacctgc agcaccgccc acgggctgag
     2521 cagctgcggg gcttcggcga cggtccggac cagggtcggc cacgccaccc atgctacgtc
     2581 catcacctcg gtgggatcgg gtgccagccc ggccgggtcc actcggccca cccagaccgg
     2641 gcagagctcg ttctccaccg tcccgccggc gtccaccgcg cggtagctga acgacgccag
     2701 cgcgagccga aggtcggtcg ggacctgacc gagttcgtcg ccgacccgcc ggaccaccgc
     2761 gtcgcgcggc gactcgccgg ggcgcggatg cccgcagcag ctgttggtcc acaccccggg
     2821 ccaggtcgcc ttgccgagcg cccgccgggt gagcagcacc cgacccgctg tgtcgaacag
     2881 atagctggag aacgccaggt ggtacggggt gtcgccggtg tggacgctga ctcgatccgc
     2941 cgccccgctg ggccggccgg cgtcgtcgag cagcaccacc tggtcagcca cggcccgcac
     3001 tcgtggtcgc caagggcgcg atatccgccg ggcgggcggt gcgacggctc ctcgcatggg
     3061 cggtcgcggc caggatcgcg attccggcgg cgatcgccgc cacggcggtc gcggtggagt
     3121 acagcgggcc gcccaggcgt cccgcggcga tccagccgac cgcccaggtc atggcagccg
     3181 cgatcgccca gcgtccgtcc agccggaacg cgtagaccgc acccagcaac gccgccaccg
     3241 ccaggacgag gaccgcgagc gcttcggacc agattccgcc ggggtcgacc ccggcgtcga
     3301 ccagcaccgc ggtgatgttc gccacagttg cgacactcac ccagccgagg tagacaccga
     3361 aggttccgtc caccaccacc gcctcgaccc gggaggcggg cgggctctgc ccgagcctct
     3421 gcaccagcag accgagaacc acggcgaggg tgaggatcac caccacgctc agccagagca
     3481 agccgacctg ggtcacgccg agccaggccg cgttcagcag catcgacagc gccgccaacc
     3541 agctgatccg cgcggcccgg gcgttgccgc gggcccacca ctgccagacc acataggcga
     3601 tcaggccgag gtagatcagc gaccagatcg agaacgccgt gttggcaggc gccaccaagg
     3661 tcgcctggga ggacaacgcg ccgccggagg actcctccac ccgggtgccc agcaggccga
     3721 agccggccag cgtcccgatc accatcacca cagcggaggc ggtgaccaga ctggctcgga
     3781 ggatcggatc cgatgtcaaa ggttgctcct gtctagatac ttgaactctg gacaacaaga
     3841 gtgccctacc gcgcaacctt agacaagctg gatgcgcaac attgcacatt ctttgctcac
     3901 actgtcgctc ttgcgcgtcc cgagccatag actctgcggc gagatggtca cgatcagtca
     3961 ggcggcacaa ctcaccggcg tcgccgaaca cacgctgagg gcctgggagc gccgctacgg
     4021 ggccttccag ccctcccgga cgcgcggcgg ctaccgggtc tacggcgacg acgcgctgca
     4081 ccggattcgc accatgcggg aactg
//
LOCUS       DAOSUG010000259         4099 bp    DNA     linear   ENV 10-JUN-2024
DEFINITION  MAG TPA_asm: Micropruina sp. isolate AMR_MDS_2195
            NODE_9157_length_4099_cov_4.124876, whole genome shotgun sequence.
ACCESSION   DAOSUG010000259 DAOSUG010000000
VERSION     DAOSUG010000259.1
DBLINK      BioProject: PRJNA925477
            BioSample: SAMN32864262
            Sequence Read Archive: SRR4236673
KEYWORDS    WGS; ENV; Metagenome Assembled Genome; MAG; Third Party Data; TPA;
            TPA:assembly.
SOURCE      Micropruina sp. (wastewater metagenome)
  ORGANISM  Micropruina sp.
            Bacteria; Bacillati; Actinomycetota; Actinomycetes;
            Propionibacteriales; Nocardioidaceae; Micropruina.
REFERENCE   1  (bases 1 to 4099)
  AUTHORS   Abdulkadir,N., Saraiva,J.P., Zhang,J., Stolte,S., Gillor,O.,
            Harms,H. and Rocha,U.
  TITLE     Genome-centric analyses of 165 metagenomes show that mobile genetic
            elements are crucial for the transmission of antimicrobial
            resistance genes to pathogens in activated sludge and wastewater
  JOURNAL   Microbiol Spectr 12 (3), e0291823 (2024)
   PUBMED   38289113
REFERENCE   2  (bases 1 to 4099)
  AUTHORS   Abdulkadir,N.
  TITLE     Direct Submission
  JOURNAL   Submitted (24-MAR-2023) Environmental Microbiology, Helmholtz
            Center for Environmental Research -UFZ, Permoserstr 15, Leipzig
            04318, Germany
COMMENT     The annotation was added by the NCBI Prokaryotic Genome Annotation
            Pipeline (PGAP). Information about PGAP can be found here:
            https://www.ncbi.nlm.nih.gov/genome/annotation_prok/
            
            ##Genome-Assembly-Data-START##
            Assembly Method        :: SPAdes v. 3.11.1
            Genome Representation  :: Full
            Expected Final Version :: Yes
            Genome Coverage        :: Not Applicable
            Sequencing Technology  :: Illumina MiSeq
            ##Genome-Assembly-Data-END##
            
            ##Genome-Annotation-Data-START##
            Annotation Provider               :: NCBI
            Annotation Date                   :: 05/04/2023 04:57:10
            Annotation Pipeline               :: NCBI Prokaryotic Genome
                                                 Annotation Pipeline (PGAP)
            Annotation Method                 :: Best-placed reference protein
                                                 set; GeneMarkS-2+
            Annotation Software revision      :: 6.5
            Features Annotated                :: Gene; CDS; rRNA; tRNA; ncRNA
            Genes (total)                     :: 2,776
            CDSs (total)                      :: 2,750
            Genes (coding)                    :: 2,714
            CDSs (with protein)               :: 2,714
            Genes (RNA)                       :: 26
            tRNAs                             :: 24
            ncRNAs                            :: 2
            Pseudo Genes (total)              :: 36
            CDSs (without protein)            :: 36
            Pseudo Genes (ambiguous residues) :: 0 of 36
            Pseudo Genes (frameshifted)       :: 2 of 36
            Pseudo Genes (incomplete)         :: 32 of 36
            Pseudo Genes (internal stop)      :: 2 of 36
            ##Genome-Annotation-Data-END##
FEATURES             Location/Qualifiers
     source          1..4099
                     /organism="Micropruina sp."
                     /mol_type="genomic DNA"
                     /submitter_seqid="NODE_9157_length_4099_cov_4.124876"
                     /isolate="AMR_MDS_2195"
                     /isolation_source="Activated sludge of municipal
                     wastewater treatment plants"
                     /db_xref="taxon:2737536"
                     /environmental_sample
                     /geo_loc_name="Canada"
                     /lat_lon="49.25 N 123.1 W"
                     /collection_date="2015-06-16"
                     /metagenome_source="wastewater metagenome"
                     /note="metagenomic"
     gene            <1..365
                     /locus_tag="PLF56_09010"
     CDS             <1..365
                     /locus_tag="PLF56_09010"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_013862012.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=3
                     /transl_table=11
                     /product="30S ribosomal protein S5"
                     /protein_id="HOQ53737.1"
                     /translation="AIAKGVEEAKKAFFRVPRIQGTIPHPVQGEKAAGVVMLRPASPG
                     TGVIAGGSARAVLECAGVHDVLAKSLGSDNAINVVHATVAALKSLEEPEQVAKRRGLP
                     VEDVAPAALLRAREEAKH"
     gene            365..547
                     /gene="rpmD"
                     /locus_tag="PLF56_09015"
     CDS             365..547
                     /gene="rpmD"
                     /locus_tag="PLF56_09015"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_002514851.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_component: GO:0000315 - organellar large ribosomal
                     subunit [Evidence IEA];
                     GO_component: GO:0022625 - cytosolic large ribosomal
                     subunit [Evidence IEA];
                     GO_function: GO:0003735 - structural constituent of
                     ribosome [Evidence IEA];
                     GO_process: GO:0006412 - translation [Evidence IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="50S ribosomal protein L30"
                     /protein_id="HOQ53738.1"
                     /translation="MSTIKVTQVKSGVGRKQNQRETLRSLGLKRIGDSVVIEDRPELV
                     GMAKSIPHLVTVEEVD"
     gene            549..992
                     /gene="rplO"
                     /locus_tag="PLF56_09020"
     CDS             549..992
                     /gene="rplO"
                     /locus_tag="PLF56_09020"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_020388091.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_component: GO:0000315 - organellar large ribosomal
                     subunit [Evidence IEA];
                     GO_component: GO:0022625 - cytosolic large ribosomal
                     subunit [Evidence IEA];
                     GO_function: GO:0003735 - structural constituent of
                     ribosome [Evidence IEA];
                     GO_process: GO:0006412 - translation [Evidence IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="50S ribosomal protein L15"
                     /protein_id="HOQ53739.1"
                     /translation="MTLKLHHLRPAPGAKTEKTRVGRGEGGKRGKTAGRGTKGSGARK
                     NMPENFEGGQMPLHMRLPKLRGFKNPFRVEYQVVNVAKLGELFPEGGDVGVDELVAKG
                     AVRDGNLVKVLGNGEVSVALKVSAHAFSASAKTKIEAAGGTATQL"
     gene            1344..2081
                     /locus_tag="PLF56_09025"
     CDS             1344..2081
                     /locus_tag="PLF56_09025"
                     /inference="COORDINATES: protein motif:HMM:NF019349.2"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="FCD domain-containing protein"
                     /protein_id="HOQ53740.1"
                     /translation="MASEHLHHAALDQIGQEIVDGTAPVGSVLTLAGLEQRFVVSRTV
                     AREIMRILESLGMVHSRRRVGITIRPRSSWNVLDPLVIRWRLRGTEHDRVVLDVNELR
                     ASLQPMAAELAARNATAEQRERLIELADEIVRTSRQPEKADARAEAELDFNTLLLRAT
                     ANELFAGFSGMVRLAQRMDGTSHVEPGLVWAQRYRRIAAAVAAGDGETARVASRAQVA
                     QGLAAMTARVGPRPAIAEQPTADDWSV"
     gene            2186..3496
                     /gene="secY"
                     /locus_tag="PLF56_09030"
     CDS             2186..3496
                     /gene="secY"
                     /locus_tag="PLF56_09030"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_020388090.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_component: GO:0005886 - plasma membrane [Evidence IEA];
                     GO_function: GO:0015450 - protein-transporting ATPase
                     activity [Evidence IEA];
                     GO_process: GO:0043952 - protein transport by the Sec
                     complex [Evidence IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="preprotein translocase subunit SecY"
                     /protein_id="HOQ53741.1"
                     /translation="MLSAFWNALRTPDLRRKIFFTLAILAIFRLGSVIPTPNVNMVNV
                     NACRLEATTGNNAGLYSMINLFSGGALLQLAIFALGIMPYITASIILQLLTVVIPRLE
                     TLKKEGASGTAKITQYTRYLTLVLAVLNATAFVTLAVNGQLFQGCSRDLVYDKGIFAI
                     VVMVLTMTAGTGLIMWMGELITERGIGNGMSVMIFTQIAAQFPASLWAIKESREGAAG
                     WLAFATVMAVGLLVMLGVIFMEQAQRRIPVQYAKRMVGRRLFGGTTTYIPMKVNQAGV
                     IPVIFASSMLYLPVLYAQFQPDNPVSSWIQTHLVRGTGWWYNSIFFVLIIAFTYFYVA
                     ITFNPEEVAGNMRKYGGFIPGIRAGKPTEEYLAYVLSRLTAPGSLYLAVISLIPAIAF
                     IFFNAQNNFPFGGTSILIVVGVGLDTVKQIESQLQQRNYEGFLR"
     gene            3517..4092
                     /locus_tag="PLF56_09035"
     CDS             3517..4092
                     /locus_tag="PLF56_09035"
                     /EC_number="2.7.4.3"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_013862016.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_function: GO:0005524 - ATP binding [Evidence IEA];
                     GO_function: GO:0019205 - nucleobase-containing compound
                     kinase activity [Evidence IEA];
                     GO_process: GO:0006139 - nucleobase-containing compound
                     metabolic process [Evidence IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="adenylate kinase"
                     /protein_id="HOQ53742.1"
                     /translation="MRLLIMGPPGAGKGTQAAIIGEHYQIPAISTGNIFRTAITERTP
                     LGERIKRIISEGGYVPDKLSVQVVVKRLEQPDAEKGWLLDGFPRTVGQVKALDAELAE
                     HGFLLDAVVSLTADEEAIVQRMLKRAEIEGRPDDNEETIRTRLEVYHEQTDPLLDIYR
                     ERGLLIEVDGMGEVSEVSQRVLAALDERVSS"
BASE COUNT          701 a         1347 c         1366 g          685 t
ORIGIN      
        1 cggcgatcgc caagggcgtc gaagaggcca agaaggcgtt cttccgggtg ccccgcatcc
       61 agggcacgat tccgcatccc gtccagggcg agaaggcggc cggcgtggtc atgctgcgtc
      121 cggcttcacc gggtaccggc gtgatcgccg gcggctcggc ccgtgcggtg ctcgaatgcg
      181 ccggcgtgca cgatgtgctc gccaagtcgc tgggctccga caacgcgatc aacgtggtgc
      241 acgccacggt ggccgcgctg aagagcctcg aagagcctga gcaggtcgcg aagcgtcgcg
      301 ggcttcccgt cgaggacgtg gccccggccg cgttgctgcg cgcccgtgag gaggcgaagc
      361 actgatgagc acgatcaagg tgacccaggt caagtccggc gtgggtcgca agcagaacca
      421 gcgtgagacc ctacgttcgc tcggtctcaa gcgcatcggt gactcggtgg tgatcgaaga
      481 ccgtcccgag ctcgtcggaa tggccaagtc gattccgcac ctggtcaccg tcgaggaggt
      541 tgactgacat gacgttgaag ttgcatcatt tgcgacccgc accgggcgcg aagaccgaaa
      601 agacccgggt cggccgtggt gagggtggca agcgcggcaa gaccgccggc cgcggcacca
      661 agggctccgg agcgcgcaag aacatgcccg agaacttcga gggcgggcag atgccgctgc
      721 atatgcggct gcccaagctg cgcggcttca agaacccgtt ccgggtcgaa taccaggtgg
      781 tgaacgtcgc caagctgggc gagctgttcc ctgagggtgg cgacgtcggc gtcgacgagc
      841 tggtcgccaa gggcgcggtg cgtgacggca acctggtcaa ggtgctgggc aacggcgagg
      901 tgtcggtggc cctgaaggtg agcgcgcacg cgttctccgc ctcggccaag accaagatcg
      961 aggctgcggg cggcaccgcc acgcagctct gatccgagag ttcactcgca cggggcggga
     1021 cgacccaccg gtcgtcccgc ccagtgctct gttcccgccg agtgctccgc tccggtacgg
     1081 gtgccctgtt cgcgccgggt gccctgcccc ggcacgggtg ctctgctccg gcacgggtgc
     1141 cccctatcgg cccggcttca tcggacgagg atcacgggcc gtgcggccgc cttccccgtg
     1201 gagctcgacg gctactctat gaaaaagctg atcaatcgcc ggttgagcgt gtcgagacgg
     1261 atgcgaccag gggtttcgac aatctcaccc aatggagtgc gtgtgtaatc agcgcatccc
     1321 cgagccggac gttaggacac cccttggcct ccgaacacct gcaccacgcc gccctcgatc
     1381 agatcgggca agagatcgtc gacggcaccg cgccggtcgg ttcggtgttg accctggccg
     1441 ggctcgagca gcgcttcgtg gtgtcacgca ccgtcgcccg cgagatcatg cgaatcctgg
     1501 aatccctcgg catggtgcac tcacgtcgcc gggtgggcat cacgatccgg ccacggtcca
     1561 gctggaacgt gctcgatccg ctcgtgatcc gctggcggct gcgcggcacc gagcacgacc
     1621 gggtggtgct cgacgtgaac gaactgcggg cctccctgca gccgatggct gccgaactgg
     1681 cggcgcgcaa cgccaccgcc gagcaacgcg aacggttgat cgaactggcg gacgagatcg
     1741 tccgcaccag tcgccagccc gagaaggcgg acgctcgcgc cgaggccgag ctcgacttca
     1801 acaccctgct gctgcgggcc accgccaacg agttgttcgc cggcttctcg ggcatggtgc
     1861 ggctggccca acgcatggac gggacgtcgc acgtcgaacc cgggctggtc tgggcgcagc
     1921 gctaccggcg gatcgcggcg gccgtcgccg caggtgacgg cgagacggcg cgagtcgcgt
     1981 cccgggccca ggtggcgcaa gggctggccg cgatgacggc ccgggtcggg ccgcgtccgg
     2041 caatcgccga acaacccacc gcggacgact ggtccgtgta gtggcgtggg gcccaggcca
     2101 cgagccggcg tccagaactt ggtagagttt cccgggttgt gtgcggtctc gcgcgcggcc
     2161 accatatgtg aggagtactg gtcggatgct gtcggccttc tggaacgcgc tgagaacacc
     2221 tgacctgcgc cgcaagattt tcttcaccct cgcgatcctg gcgatcttca ggctcggctc
     2281 ggtgatcccc acgcccaacg tgaacatggt caacgtgaac gcctgtcggc tcgaggcgac
     2341 caccggcaac aacgccgggc tgtactcgat gatcaacctg ttctcgggcg gggcgctgct
     2401 gcaactggcc atcttcgcgc tgggcatcat gccctacatc accgcgagca tcattctgca
     2461 gctgctcacg gtcgtcatcc cgaggctgga gaccctcaag aaggagggcg cctccggcac
     2521 cgcgaagatc acccagtaca cccgctacct gaccctggtg ctggcggtgc tgaacgccac
     2581 cgcgttcgtc accctggcgg tgaacggcca gctgttccag ggctgctcgc gtgacctggt
     2641 ctacgacaag ggcatctttg cgatcgtggt gatggtgctc acgatgaccg cgggcaccgg
     2701 cctgatcatg tggatgggcg agctgatcac cgagcgcggt atcggcaacg gcatgtcggt
     2761 gatgatcttc acccagatcg ccgcgcagtt cccggcctcg ctgtgggcga tcaaagagag
     2821 ccgtgagggg gcggccggct ggctcgcgtt cgcgaccgtg atggctgtcg gcctgctggt
     2881 gatgctgggc gtgatcttca tggagcaggc gcagcgacgc atcccggtgc agtacgccaa
     2941 acgcatggtc ggtcggcggc tgttcggcgg caccaccacc tacattccga tgaaggtcaa
     3001 ccaggccggc gtcattccgg tgatcttcgc cagctcgatg ctgtacctgc ccgtgctgta
     3061 cgcccagttc cagcccgaca acccggtgtc gtcgtggatt cagacccacc tggtgcgcgg
     3121 caccggctgg tggtacaact ccatcttctt cgtgctgatc atcgccttca cctacttcta
     3181 cgtggcgatc accttcaacc ccgaagaggt cgccggcaac atgaggaaat acggcggctt
     3241 cattccgggc atccgcgccg gtaagccgac cgaggagtac ctggcctacg tgctgtcgcg
     3301 tctgaccgct cccggctcgc tgtacctggc cgtcatttcg ctgattcccg cgatcgcgtt
     3361 catatttttc aatgcacaga acaacttccc cttcggtggc actagcatcc tgatcgtggt
     3421 cggagttggc cttgacacgg tcaaacagat cgagagccag cttcagcaac gcaactacga
     3481 aggattcctg cggtaactca cgggcgaggg gctcgaatgc gactgttgat catgggcccg
     3541 cccggcgcgg gtaaaggaac gcaagccgcc atcatcggcg agcactacca gattcccgcg
     3601 atctccaccg gcaacatctt ccgcacggcg atcaccgaac gcacgccctt gggcgagcgc
     3661 atcaagcgca tcatctccga gggcgggtac gtgcccgaca agctgagcgt gcaagtggtg
     3721 gtcaagcggc tcgagcagcc cgacgccgag aagggctggc tgctcgacgg cttcccccgc
     3781 accgtgggtc aggtcaaggc gctcgacgcc gaactcgccg agcacggctt cctgctcgac
     3841 gcggtggtgt cgctgaccgc cgacgaagag gcgatcgtgc agcgcatgct caagcgggcc
     3901 gagatcgaag gccgtcccga cgacaacgaa gagaccattc gcacccggct cgaggtttac
     3961 cacgagcaga ccgatccgct gctcgacatc taccgcgagc gcggtctact gatcgaggtg
     4021 gacggcatgg gtgaggtctc cgaggtctcg cagcgggtgc tggccgctct cgacgagcga
     4081 gtcagcagct gaccgttgg
//
LOCUS       DAOSUG010000260         4090 bp    DNA     linear   ENV 10-JUN-2024
DEFINITION  MAG TPA_asm: Micropruina sp. isolate AMR_MDS_2195
            NODE_9177_length_4090_cov_3.938786, whole genome shotgun sequence.
ACCESSION   DAOSUG010000260 DAOSUG010000000
VERSION     DAOSUG010000260.1
DBLINK      BioProject: PRJNA925477
            BioSample: SAMN32864262
            Sequence Read Archive: SRR4236673
KEYWORDS    WGS; ENV; Metagenome Assembled Genome; MAG; Third Party Data; TPA;
            TPA:assembly.
SOURCE      Micropruina sp. (wastewater metagenome)
  ORGANISM  Micropruina sp.
            Bacteria; Bacillati; Actinomycetota; Actinomycetes;
            Propionibacteriales; Nocardioidaceae; Micropruina.
REFERENCE   1  (bases 1 to 4090)
  AUTHORS   Abdulkadir,N., Saraiva,J.P., Zhang,J., Stolte,S., Gillor,O.,
            Harms,H. and Rocha,U.
  TITLE     Genome-centric analyses of 165 metagenomes show that mobile genetic
            elements are crucial for the transmission of antimicrobial
            resistance genes to pathogens in activated sludge and wastewater
  JOURNAL   Microbiol Spectr 12 (3), e0291823 (2024)
   PUBMED   38289113
REFERENCE   2  (bases 1 to 4090)
  AUTHORS   Abdulkadir,N.
  TITLE     Direct Submission
  JOURNAL   Submitted (24-MAR-2023) Environmental Microbiology, Helmholtz
            Center for Environmental Research -UFZ, Permoserstr 15, Leipzig
            04318, Germany
COMMENT     The annotation was added by the NCBI Prokaryotic Genome Annotation
            Pipeline (PGAP). Information about PGAP can be found here:
            https://www.ncbi.nlm.nih.gov/genome/annotation_prok/
            
            ##Genome-Assembly-Data-START##
            Assembly Method        :: SPAdes v. 3.11.1
            Genome Representation  :: Full
            Expected Final Version :: Yes
            Genome Coverage        :: Not Applicable
            Sequencing Technology  :: Illumina MiSeq
            ##Genome-Assembly-Data-END##
            
            ##Genome-Annotation-Data-START##
            Annotation Provider               :: NCBI
            Annotation Date                   :: 05/04/2023 04:57:10
            Annotation Pipeline               :: NCBI Prokaryotic Genome
                                                 Annotation Pipeline (PGAP)
            Annotation Method                 :: Best-placed reference protein
                                                 set; GeneMarkS-2+
            Annotation Software revision      :: 6.5
            Features Annotated                :: Gene; CDS; rRNA; tRNA; ncRNA
            Genes (total)                     :: 2,776
            CDSs (total)                      :: 2,750
            Genes (coding)                    :: 2,714
            CDSs (with protein)               :: 2,714
            Genes (RNA)                       :: 26
            tRNAs                             :: 24
            ncRNAs                            :: 2
            Pseudo Genes (total)              :: 36
            CDSs (without protein)            :: 36
            Pseudo Genes (ambiguous residues) :: 0 of 36
            Pseudo Genes (frameshifted)       :: 2 of 36
            Pseudo Genes (incomplete)         :: 32 of 36
            Pseudo Genes (internal stop)      :: 2 of 36
            ##Genome-Annotation-Data-END##
FEATURES             Location/Qualifiers
     source          1..4090
                     /organism="Micropruina sp."
                     /mol_type="genomic DNA"
                     /submitter_seqid="NODE_9177_length_4090_cov_3.938786"
                     /isolate="AMR_MDS_2195"
                     /isolation_source="Activated sludge of municipal
                     wastewater treatment plants"
                     /db_xref="taxon:2737536"
                     /environmental_sample
                     /geo_loc_name="Canada"
                     /lat_lon="49.25 N 123.1 W"
                     /collection_date="2015-06-16"
                     /metagenome_source="wastewater metagenome"
                     /note="metagenomic"
     gene            complement(207..1229)
                     /locus_tag="PLF56_09040"
     CDS             complement(207..1229)
                     /locus_tag="PLF56_09040"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_011753692.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_function: GO:0000976 - transcription cis-regulatory
                     region binding [Evidence IEA];
                     GO_function: GO:0003700 - DNA-binding transcription factor
                     activity [Evidence IEA];
                     GO_function: GO:0043565 - sequence-specific DNA binding
                     [Evidence IEA];
                     GO_process: GO:0006351 - DNA-templated transcription
                     [Evidence IEA];
                     GO_process: GO:0006355 - regulation of DNA-templated
                     transcription [Evidence IEA];
                     GO_process: GO:0045892 - negative regulation of
                     DNA-templated transcription [Evidence IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="LacI family DNA-binding transcriptional
                     regulator"
                     /protein_id="HOQ53743.1"
                     /translation="MPRPTLQSIADTVGVSRMTVSNAFSRPDQLSSELRDRILAVAAE
                     VGYHGPDPAARSLATGTSGTIGVLWTQSLRLALSDPVTAGFLGALADELSADGLALTL
                     LPPNVPAHDVAMDAAVAYSSPPDSEAMQWLRGRGVPMVGVDMPWPDGPQVAIDDRGGA
                     RAAAELLIELGHRRVLVLAQAGELQNEWPLDERVTGWLEGLGPVTPVVGRVQAFGDNQ
                     AVIVDLLRGVAPTAVLCVTDLVAAQAVAAASELGLDVPGQLSVIGFDDHPVAAGLGLT
                     TVRQNVVAKGRAAAHLVRQLLARAGEGGAGTDADPSRPADVLLPVELVVRQSTGPAPA
                     ETARTS"
     gene            1306..2958
                     /locus_tag="PLF56_09045"
     CDS             1306..2958
                     /locus_tag="PLF56_09045"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_017933189.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="glycoside hydrolase family 13 protein"
                     /protein_id="HOQ53744.1"
                     /translation="MDGRNAGNDWWRHAVVYQIYPRSFADANGDGVGDVAGIRSKLPY
                     LAALGIDAIWISPWYPSPMKDAGYDVSDYRDIEPVFGTLAEADAMVAEAHDLGIKVII
                     DIVPNHTSDQHVLFQQALAAGRGSAERERFIFRDGRGENGDLPPNDWQSTFGGPAWTR
                     VADGQWYLHLFTPEQADVNWRNADIKAEFEQTLRFWFDRGIDGFRIDVAHGLEKDWAL
                     PDLNGLEFPLEEGAENEHPHWDQPSVHDIFREWRVIADEYTPSRAYCGEIWVGRAHRL
                     AAYLRPDELHTAFNFDFLMAPFLPSTMRAVIDATIASHAAVGAPPTWVLGNHDVARPV
                     SRYAREQVGLEPMARNLVHFEHLPADFDLGRRRARAAALLMFGLPGSSYVYEGEELGL
                     PEVEDIPAEALQDPVWTMSGNTDKGRDGCRVPLPWTSAGYDYGFSPEGGAEPWLPQPK
                     EWAALAADVQDGTPGSTLELYRAALKLRRGLSQLGDGVLEWLDAPEGVLAYRRPGEGG
                     GFQSWTNFGPDPVALPAGELLLASGDLVNGQLPADTTAWLRV"
     gene            3283..3597
                     /locus_tag="PLF56_09050"
     CDS             3283..3597
                     /locus_tag="PLF56_09050"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_019048788.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="SelT/SelW/SelH family protein"
                     /protein_id="HOQ53745.1"
                     /translation="MTPRHDSPIVTIRYCTGCRWLLRAQWYQGELLQTFTDVLGGVLL
                     VPSSGAMFRIEVGEVTVWNRKTDGGFPEITALKRLVRDLVAPDMSLGHSDDPTPPSDT
                     FS"
     gene            complement(3803..>4090)
                     /locus_tag="PLF56_09055"
     CDS             complement(3803..>4090)
                     /locus_tag="PLF56_09055"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_008356290.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="cation acetate symporter"
                     /protein_id="HOQ53746.1"
                     /translation="RFNTRGSVWSIYGGLISAVGLIAFSPVVSGLKSSMFPGVNFAWF
                     PLTNPALISVPLGFFLGWLGTVTSREFDAAKQAEMEVRSMTGAGAEKAVTH"
BASE COUNT          666 a         1419 c         1404 g          601 t
ORIGIN      
        1 gccgattggc ggcgatcagc ggcgtactcg tcatgccggg catgccccgg ggtctcggcg
       61 cacgcagaga tcccggcatg cgccgggatg acggtggtca cgacattgat ggaagcgctg
      121 atcaacgcgc ggcgtactcc tcatccctgc attgcccagg atctcgacgc acgtagagat
      181 cgcgacgtgg gccgggatga caagcgtcac gacgtcctag cggtctcagc gggtgccggg
      241 ccggtgctct gccgcaccac caactcgacc ggcaacagca cgtcggccgg ccgcgacggg
      301 tcggcgtcgg tgccggcgcc gccctcgccg gcacgggcca acagttgccg caccaggtga
      361 gcggccgcgc gacccttcgc aacgacattc tggcgcaccg tggtcaggcc caggccggcc
      421 gcgacggggt gatcgtcgaa cccgatcacc gagagctgcc cgggtacgtc cagccccagc
      481 tcggacgccg ccgccaccgc ctgagccgcc accagatcgg tcacgcacaa cacggcggtg
      541 ggcgccaccc cccgcagcag gtcgacgatg acggcctgat tgtcaccgaa ggcctgcacc
      601 cggcccacca cgggtgtcac cggccccagc ccctccagcc agccggtgac ccgttcgtcc
      661 agcggccact cgttctgcag ctcgccggcc tgggcgagca ccagcacccg gcgatgcccc
      721 agctcgatca gaagttcagc cgccgcccga gccccgcccc ggtcgtcgat cgcgacctgg
      781 gggccgtcgg gccagggcat gtcgacgccc accatgggca ccccccgtcc gcgcaaccac
      841 tgcatggcct ccgaatcggg gggtgacgag taggcgacgg cagcgtccat ggccacgtcg
      901 tgggcgggca cgttcggcgg cagcagggtc agcgcgagac cgtccgcgct cagttcatcg
      961 gccagcgccc ccaggaatcc ggcggtcacc gggtcggaca gcgccagccg cagcgactgc
     1021 gtccacaaca cgccgatcgt gccggatgtg ccggtggcca acgaccgcgc tgccggatcg
     1081 ggcccgtgat agcccacctc ggcggcgacg gcaagaattc gatcgcgcaa ctcggacgac
     1141 aactgatccg gacgcgagaa tgcgttcgac accgtcatgc ggctgacccc gacggtgtcg
     1201 gcgatcgact gcagcgttgg ccgcggcatg cgccctcctc gacttgtgga cagtgcctcc
     1261 aggcactatc tttaccggta cagactatac cggtaaagga gttccatgga cgggcgcaac
     1321 gcaggcaacg actggtggcg gcacgcggtg gtgtaccaga tctatccgcg cagcttcgcc
     1381 gacgccaacg gcgacggcgt gggcgacgtc gcaggcattc gcagcaagtt gccctacctg
     1441 gcggcgctcg gcatcgacgc gatctggatc agcccctggt acccgtcccc gatgaaggac
     1501 gccggctacg acgtcagcga ctaccgcgac atcgagccgg tgttcggcac cttggccgag
     1561 gcggacgcga tggtggccga ggcccacgac ctgggcatca aggtgatcat cgacatcgtc
     1621 ccgaaccaca ccagcgacca gcacgtgctg tttcagcagg cactcgccgc cggccgtggc
     1681 tcggccgaac gtgagcggtt catcttccgt gacgggcgcg gcgagaacgg cgacctgccc
     1741 cccaacgact ggcagagcac cttcggcggc ccggcctgga cccgggtcgc ggacggacag
     1801 tggtacctgc acctgttcac ccccgagcag gccgacgtca actggcgcaa cgccgacatc
     1861 aaggccgagt tcgagcagac cctgcggttc tggttcgacc gcggtatcga cggcttccgc
     1921 atcgacgtgg cacacgggct cgagaaggat tgggcgctgc ccgacctcaa cggtctggag
     1981 ttcccgctcg aagaaggcgc cgagaacgag cacccgcact gggaccagcc gagcgtgcac
     2041 gacatcttcc gcgagtggcg agtgatcgcc gacgagtaca ccccgtcccg ggcgtattgc
     2101 ggtgagatct gggtcggccg tgcgcatcgc ctggcggcct accttcgtcc ggacgagttg
     2161 cacaccgcct tcaacttcga cttcttgatg gctccgttcc tgcccagcac gatgcgcgcg
     2221 gtgatcgatg ccacgatcgc gtcgcatgcc gccgtcggcg ccccgcccac ctgggtgctc
     2281 ggcaaccacg acgtcgcccg tccggtgtcg cgctacgcgc gcgagcaggt cggtctggag
     2341 ccgatggcgc gcaacctggt gcactttgag cacctgccgg ccgatttcga tctcggacgc
     2401 cgccgcgccc gcgccgcagc gctgctgatg ttcgggctgc ccggcagttc gtatgtgtac
     2461 gagggcgaag agctgggcct gcccgaggtc gaggacattc cggccgaggc gctgcaggac
     2521 ccggtctgga ccatgtcggg gaacaccgac aagggccggg acggctgccg cgtgccgctg
     2581 ccctggacct cagccggcta tgactacggg ttctcgcccg agggtggcgc cgagccgtgg
     2641 ctgccgcagc cgaaggagtg ggccgcactg gccgccgacg tgcaggacgg cacgccaggc
     2701 tcgacgctcg agctgtaccg ggccgcgctg aagctgcgcc gggggttgag ccaactgggc
     2761 gacggggtgc tcgaatggct ggacgcgccg gaaggcgtgc ttgcctaccg gcgcccgggt
     2821 gagggcggcg ggttccagag ctggacgaac ttcggccccg acccggtggc gttgccggcc
     2881 ggtgagctgc tgttggcctc gggcgacctg gtcaatggtc aactgccggc cgacaccacg
     2941 gcctggctgc gcgtttgatt gtgagcgggt gggttttggc acagcagtcg gtgccaaaac
     3001 ccacccactc tttccatggc actggaccgg ccgggcgaaa caaagctcga atcggctgta
     3061 tcagcgttgg ggcggcgtgt acctagtggt tgaagtcgct gatcccgagg gtgctggggg
     3121 gacgaatcgg tggtgcgaca agggggaacg cacggtgcgc gacgcaccgg cgcaaccggt
     3181 ccgttcgggg ggaggggccg gtagccggtg tgtcgcgcac gtgcgccgcc ggtggcggcc
     3241 ggctcgcccg cacgaccgcc accacctgac acaatcgatg gcgtgacccc acgccatgac
     3301 tctccgatcg tcaccattcg ctactgcacg ggttgccgct ggctgctgcg ggcacagtgg
     3361 taccaaggcg agttgctgca gaccttcacc gacgttctcg gcggggtgct gctggtgccc
     3421 tccagcggtg cgatgttccg catcgaggtg ggcgaggtga cggtctggaa ccgcaagacc
     3481 gacggcggct ttcccgagat cacggcactg aagcgcctgg tgcgtgacct ggtcgcgccc
     3541 gacatgtcgc tcggccattc cgacgatccg acaccgccca gcgacacctt ctcctgaacc
     3601 tgcatccacg tatgggtgcc ttcgcgagcg gcaccagacg ggtggactgg caagccgtcc
     3661 gcgcgagggc atagcgggtc tgccttgcgt agccggcgac gccagggggc ccgaatggcg
     3721 ccgtgcgccc ccctaaccat cactggcccg cgaagctacg cgggccagag atagttaggg
     3781 gggcggttgc agtaggcgca gatcagtgcg tgacggcctt ctcggcgccc gcgccggtca
     3841 tcgagcgaac ctccatctcg gcctgcttgg cggcgtcgaa ctcgcggctg gtgaccgtgc
     3901 cgagccagcc aaggaaaaag ccgagcggta ccgagatcag cgccgggttg gtgagcggga
     3961 accacgcgaa gttcaccccg gggaacatcg atgacttcag ccccgacacc actggcgaga
     4021 aggcgatcag cccgaccgcc gagatcaggc cgccgtagat cgaccacacc gagccgcggg
     4081 tgttgaaccg
//
LOCUS       DAOSUG010000261         4085 bp    DNA     linear   ENV 10-JUN-2024
DEFINITION  MAG TPA_asm: Micropruina sp. isolate AMR_MDS_2195
            NODE_9187_length_4085_cov_3.908685, whole genome shotgun sequence.
ACCESSION   DAOSUG010000261 DAOSUG010000000
VERSION     DAOSUG010000261.1
DBLINK      BioProject: PRJNA925477
            BioSample: SAMN32864262
            Sequence Read Archive: SRR4236673
KEYWORDS    WGS; ENV; Metagenome Assembled Genome; MAG; Third Party Data; TPA;
            TPA:assembly.
SOURCE      Micropruina sp. (wastewater metagenome)
  ORGANISM  Micropruina sp.
            Bacteria; Bacillati; Actinomycetota; Actinomycetes;
            Propionibacteriales; Nocardioidaceae; Micropruina.
REFERENCE   1  (bases 1 to 4085)
  AUTHORS   Abdulkadir,N., Saraiva,J.P., Zhang,J., Stolte,S., Gillor,O.,
            Harms,H. and Rocha,U.
  TITLE     Genome-centric analyses of 165 metagenomes show that mobile genetic
            elements are crucial for the transmission of antimicrobial
            resistance genes to pathogens in activated sludge and wastewater
  JOURNAL   Microbiol Spectr 12 (3), e0291823 (2024)
   PUBMED   38289113
REFERENCE   2  (bases 1 to 4085)
  AUTHORS   Abdulkadir,N.
  TITLE     Direct Submission
  JOURNAL   Submitted (24-MAR-2023) Environmental Microbiology, Helmholtz
            Center for Environmental Research -UFZ, Permoserstr 15, Leipzig
            04318, Germany
COMMENT     The annotation was added by the NCBI Prokaryotic Genome Annotation
            Pipeline (PGAP). Information about PGAP can be found here:
            https://www.ncbi.nlm.nih.gov/genome/annotation_prok/
            
            ##Genome-Assembly-Data-START##
            Assembly Method        :: SPAdes v. 3.11.1
            Genome Representation  :: Full
            Expected Final Version :: Yes
            Genome Coverage        :: Not Applicable
            Sequencing Technology  :: Illumina MiSeq
            ##Genome-Assembly-Data-END##
            
            ##Genome-Annotation-Data-START##
            Annotation Provider               :: NCBI
            Annotation Date                   :: 05/04/2023 04:57:10
            Annotation Pipeline               :: NCBI Prokaryotic Genome
                                                 Annotation Pipeline (PGAP)
            Annotation Method                 :: Best-placed reference protein
                                                 set; GeneMarkS-2+
            Annotation Software revision      :: 6.5
            Features Annotated                :: Gene; CDS; rRNA; tRNA; ncRNA
            Genes (total)                     :: 2,776
            CDSs (total)                      :: 2,750
            Genes (coding)                    :: 2,714
            CDSs (with protein)               :: 2,714
            Genes (RNA)                       :: 26
            tRNAs                             :: 24
            ncRNAs                            :: 2
            Pseudo Genes (total)              :: 36
            CDSs (without protein)            :: 36
            Pseudo Genes (ambiguous residues) :: 0 of 36
            Pseudo Genes (frameshifted)       :: 2 of 36
            Pseudo Genes (incomplete)         :: 32 of 36
            Pseudo Genes (internal stop)      :: 2 of 36
            ##Genome-Annotation-Data-END##
FEATURES             Location/Qualifiers
     source          1..4085
                     /organism="Micropruina sp."
                     /mol_type="genomic DNA"
                     /submitter_seqid="NODE_9187_length_4085_cov_3.908685"
                     /isolate="AMR_MDS_2195"
                     /isolation_source="Activated sludge of municipal
                     wastewater treatment plants"
                     /db_xref="taxon:2737536"
                     /environmental_sample
                     /geo_loc_name="Canada"
                     /lat_lon="49.25 N 123.1 W"
                     /collection_date="2015-06-16"
                     /metagenome_source="wastewater metagenome"
                     /note="metagenomic"
     gene            <1..516
                     /locus_tag="PLF56_09060"
     CDS             <1..516
                     /locus_tag="PLF56_09060"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_011756641.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_function: GO:0008483 - transaminase activity [Evidence
                     IEA];
                     GO_function: GO:0030170 - pyridoxal phosphate binding
                     [Evidence IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="aminotransferase class III-fold pyridoxal
                     phosphate-dependent enzyme"
                     /protein_id="HOQ53747.1"
                     /translation="REGVVPDLITTAKGIAGGLPLAAVTGRAELMDAVHPGGLGGTYG
                     GNPVACAAALGAIEEMETHDLPGRARQIERIFRTRLSELADDHPSIGEIRGRGAMMAV
                     ELVEPGTKRPDAGLTARVARACHARGLVVLTAGSFGNVFRFLPPLAISDDLLHEGLDI
                     IGEAFAEASAG"
     gene            complement(535..984)
                     /locus_tag="PLF56_09065"
     CDS             complement(535..984)
                     /locus_tag="PLF56_09065"
                     /inference="COORDINATES: ab initio
                     prediction:GeneMarkS-2+"
                     /note="Derived by automated computational analysis using
                     gene prediction method: GeneMarkS-2+."
                     /codon_start=1
                     /transl_table=11
                     /product="hypothetical protein"
                     /protein_id="HOQ53748.1"
                     /translation="MFNLFSVFYSLLGLLALVITGAVVTGRLRQGARTLMWSGIVMLA
                     LTHVSGLVTTAMIQARSPVFAYAALNFASVALTVVGTVLLILAIGRAAREGQPSEPSA
                     PGAPQYPIQGGGYQPQPGNQPYSGDQPYGRPQPYDQPGTWGDQQRPR"
     gene            complement(1058..2521)
                     /gene="miaB"
                     /locus_tag="PLF56_09070"
     CDS             complement(1058..2521)
                     /gene="miaB"
                     /locus_tag="PLF56_09070"
                     /EC_number="2.8.4.3"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_013161195.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_function: GO:0016782 - transferase activity,
                     transferring sulphur-containing groups [Evidence IEA];
                     GO_function: GO:0051539 - 4 iron, 4 sulfur cluster binding
                     [Evidence IEA];
                     GO_function: GO:1904047 - S-adenosyl-L-methionine binding
                     [Evidence IEA];
                     GO_process: GO:0006400 - tRNA modification [Evidence IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="tRNA (N6-isopentenyl
                     adenosine(37)-C2)-methylthiotransferase MiaB"
                     /protein_id="HOQ53749.1"
                     /translation="MTIDQQTRTYQVVTYGCQMNNHDSERIAGLLEDAGYAPHSDGQQ
                     ADLVVFNTCAVRENADNRLYGNLGYLAPVKAAHPGMQIAVGGCMAQKDRETITKRAPW
                     VDVVFGTHNIGKLLVLLERARIEHEAQVEIAEALEAFPSTLPTRRESPYSAWVSVSVG
                     CNNTCTFCIVPQLRGKETDRRPGDVLAEIRALVDDGVQEITLLGQNVNTYGVEFGDRF
                     AFGKLLRACGTIEGLERVRFTSPHPAAFTDDVIEAMAETPNVMPSLHMPLQSGSDRVL
                     KAMRRSYRSEKYLGIIERVRAAMPHAAITTDIIVGFPGETEADFAETLRVAQASRFAA
                     AFTFQYSIRPGTPAATMPDQVPKQVVQERYERLIDLVGDLAWSENKKLLGTRVEVLFA
                     AGEGRKDAATARVSGRSRDNRLVHVGLPDGAERPRPGDIGLAEISYAAPHHLVADGGV
                     TGLRRTRGGDAWDARHTPQKRLVGLGLPTLTAARSLS"
     gene            complement(2602..3423)
                     /locus_tag="PLF56_09075"
     CDS             complement(2602..3423)
                     /locus_tag="PLF56_09075"
                     /inference="COORDINATES: protein motif:HMM:NF024242.2"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_function: GO:0043565 - sequence-specific DNA binding
                     [Evidence IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="AraC family transcriptional regulator"
                     /protein_id="HOQ53750.1"
                     /translation="MTTSPPRRIDPVDRAHLTGVSRPSPPIFRYQPSSRLAQIVGRYW
                     IPVWSLSEPSTQSTLQHPVCLLVISNTYARFYGVVRGRSSVTLEGHGWAVGAMLRPAS
                     GRLLLGASVSDLVDSHVDLRALTRLDGAGLAEQITAAMATDPSDEAGHLAAITAYERA
                     LSPLLPLDDPGRLINAVIDWLTDHPDVTRVTDVAEAFGLTERTLQRLVLDRVGLSPKW
                     LIQRRRLHDAITRLKSGAGSLADLAAELGYADQAHFTTDFRTVTGMTPGQYLADQ"
     gene            3474..4028
                     /locus_tag="PLF56_09080"
     CDS             3474..4028
                     /locus_tag="PLF56_09080"
                     /inference="COORDINATES: protein motif:HMM:TIGR03083.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="TIGR03086 family metal-binding protein"
                     /protein_id="HOQ53751.1"
                     /translation="MNHTISIFRDRADRFSTILAGVNSRWDAPTPCTDWTAEDIVRHV
                     IATERDFLARHDLGSPDATADLEPAQAWDQHRRSTLDILSRDGVADRAFDGHFGPTTI
                     GDTLANFYGWDLAVHGWDIARATGQDGSITADEADNLARIAATWGEALHSEGVCGPAR
                     PVADDAGPVERLLAMVGRDPDWTD"
BASE COUNT          621 a         1402 c         1438 g          624 t
ORIGIN      
        1 cgcgagggcg tcgtgcccga cctgatcacc acggccaagg gcatcgccgg cgggttgccg
       61 ctggccgcag tcaccggccg cgccgaactg atggacgccg tgcaccccgg cggcctcggc
      121 ggcacctacg gcggtaaccc ggtcgcctgc gccgcggccc tgggcgccat cgaagagatg
      181 gagacccacg acctacccgg gcgcgcccgg cagatcgagc gcatctttcg cacccggttg
      241 agcgagctcg ccgacgacca cccctcgatc ggcgagatcc ggggacgcgg cgcgatgatg
      301 gcggtcgaac tggtcgaacc gggcaccaag cgacccgatg ccggcctcac cgcgcgggtg
      361 gcgagggcat gtcacgcgcg gggtttggtg gtgctcaccg ccggctcgtt cggcaacgtc
      421 tttcgcttcc tgccgccgct ggcgatcagc gacgacctgc tgcacgaggg cctggacatc
      481 atcggcgagg ccttcgccga ggcgtccgcg ggctgaggcg ccctcgttcc gcactcagcg
      541 tggacgctgc tggtcgcccc aggtgcccgg ctggtcgtag ggttgcggcc ggccgtaggg
      601 ctgatcaccc gaatagggct gattgcccgg ttggggctgg tacccaccgc cctggatcgg
      661 gtactgcggc gcaccgggcg cactgggttc ggacggctga ccctcgcggg cggctcgtcc
      721 gatcgccaga atcaacagaa ccgtaccgac cactgtgagt gcgacgctcg cgaaattgag
      781 cgccgcgtag gcgaacaccg ggcttcgagc ctgaatcatg gcggtggtga cgagaccgga
      841 cacatgggtg agcgccagca tcacgatgcc ggaccacatc agggtgcggg cgccctgccg
      901 gaggcgtccg gtgaccaccg cgccggtgat cacgagagcc agcagcccga gcaggctgta
      961 gaagacactg aacaggttga acatcggact cctccgtcgg tgtgccgcga tcgtacccag
     1021 cggcggtgtc gccctggcga tccctggtcg ccggcgttca gctgagggaa cgtgccgcgg
     1081 tcagggtcgg caggcccagc ccgaccagcc gcttctgggg ggtgtgccgg gcgtcccagg
     1141 cgtcaccgcc gcgggtgcgc cgcaggccgg tcactccccc gtccgcgacc aggtggtgcg
     1201 gcgcggcgta gcttatctcg gccaggccga tgtcgcccgg acgcggccgc tcagccccgt
     1261 ccggtagccc cacgtgcacc aggcggttgt cgcgcgaccg tcccgaaacg cgggcggtgg
     1321 cggcgtcctt gcggccctcg ccggccgcga acagcacctc gacccgggtg cccagaagct
     1381 tcttgttctc gctccaggcc aggtcgccga ccaggtcgat cagccgctcg tagcgctctt
     1441 ggacgacctg cttgggcacc tgatcgggca tcgtcgcggc gggggtgccg ggccggatgg
     1501 agtactgaaa ggtgaaggcg gcggcgaacc gggacgcctg cgcgacccgc aacgtctcgg
     1561 cgaaatcggc ctcggtctca cccggaaaac cgacgatgat gtcggtggtg atcgcggcgt
     1621 gcggcatggc ggcgcggacc cgctcgatga tgcccaggta cttctcggag cggtacgagc
     1681 ggcgcatggc cttgagcacc cggtcggagc cggactgcag cggcatgtgc aggctgggca
     1741 tcacgttggg cgtctcggcc atcgcttcga tcacgtcgtc ggtgaaggcc gccgggtgcg
     1801 gcgaggtgaa ccggacgcgc tccaggccct cgatcgtgcc gcaggcccgc agcagcttgc
     1861 cgaaggcgaa ccggtcaccg aactcgacgc cgtaggtgtt gacgttctga ccgagcaggg
     1921 tgatctcttg cacgccgtcg tccaccaggg cccggatttc ggccaacacg tcacccggac
     1981 ggcggtcggt ctctttgccg cgcagttgcg ggacgatgca gaacgtgcag gtgttgttgc
     2041 agccgacgct gaccgacacc caggccgagt agggcgactc gcggcgggtg ggcagggtgg
     2101 aggggaacgc ctccagcgcc tccgcgatct cgacctgcgc ctcgtgctcg atgcgggcgc
     2161 gctcgagcag caccagcaac ttgccgatgt tgtgcgtgcc gaacaccaca tcgacccagg
     2221 gggcgcgctt ggtgatggtc tcccggtcct tttgcgccat gcacccgccc acggcgatct
     2281 gcatgcccgg gtgcgcagcc ttgaccggcg ccaggtagcc gaggttgccg tagaggcgat
     2341 tgtcggcgtt ctcgcgcacc gcgcaggtgt tgaacaccac caggtcggcc tgctggccgt
     2401 cgctgtgcgg ggcgtacccg gcgtcttcga gcagcccggc gatccgttcg gagtcatgat
     2461 tgttcatctg acagccgtac gtgaccacct gataggtgcg ggtttgttgg tcgatcgtca
     2521 tctcggggcg caagcttacc gggtgtgccg ccacccacct acggccgtac gaggcacccg
     2581 tcggccggca gtgtgacctg ctcactgatc ggccaggtac tggccgggcg tcataccggt
     2641 caccgtgcga aagtcggtgg tgaagtgcgc ctggtcggcg taccccaact cggcggccag
     2701 atcggccagc gaacccgcgc cgctcttgag gcgggtgatc gcgtcgtgca gccggcgccg
     2761 ctggatcaac cacttggggc tcagcccgac ccggtccagg accaggcgct gcaaggtgcg
     2821 ttcggtcagg ccgaaggcct cggcgacatc ggtgacccgg gtgacgtccg ggtgatcggt
     2881 gagccagtcg atgaccgcat tgatcagccg tccgggatcg tccagtggaa gcagcggtga
     2941 cagcgcccgc tcgtacgcgg tgatggccgc caggtgaccg gcctcgtcgg acgggtcggt
     3001 ggccatggcc gccgtgatct gctcggcaag cccggcaccg tccagcctgg tcagtgcgcg
     3061 gaggtccacg tggctgtcga ccaggtcgct caccgacgct cccagcagca gtcgtccgga
     3121 cgccggacgc agcatcgccc cgaccgccca gccgtggccc tccaaggtca ccgatgagcg
     3181 gccacgcacc acaccgtaga accgggcgta ggtgttgctg atcaccagca ggcacaccgg
     3241 atgctgcaac gtgctctggg tggagggttc gctcagcgac cagaccggga tccagtatcg
     3301 cccgacgatc tgggccagcc gcgacgaggg ctggtagcgg aagatcggcg gcgagggacg
     3361 cgagactccc gtcaggtgcg ccctgtcgac cggatcaatg cggcgcggcg gcgacgtggt
     3421 cattgtcgga ttatcacaag ccgccggcgc acacgcctct ttaggctcgc ggcatgaacc
     3481 acaccatcag catcttccgc gaccgcgcgg accgcttctc taccatcctg gcaggtgtca
     3541 acagccgctg ggatgccccg acgccctgca cggactggac cgccgaagac atcgtccggc
     3601 acgtgatcgc gaccgaacgc gacttcttgg cgcgccacga tctgggctca ccggacgcca
     3661 ccgctgacct cgagccggcg caggcctggg accagcaccg acgcagcacc cttgacatcc
     3721 tgagtcggga cggcgtcgcc gatcgcgcct tcgacggcca cttcggcccg acgaccatcg
     3781 gtgacacgct ggccaacttc tacggctggg atcttgccgt gcacggctgg gacatcgcgc
     3841 gggccaccgg ccaggacggc agcatcaccg cggacgaagc ggacaatctc gcgcggatcg
     3901 ccgcgacctg gggcgaggcg ctgcacagcg agggggtgtg cgggccggcc cgtccggtgg
     3961 ccgacgacgc cggacctgtc gagcgtctgc tggcgatggt tggccgcgac cctgactgga
     4021 ccgactgatc gcgggcggag cgcgaatcgt ctcccgactg aatcgtgcac ccgtctgaat
     4081 cgtgc
//
LOCUS       DAOSUG010000262         4074 bp    DNA     linear   ENV 10-JUN-2024
DEFINITION  MAG TPA_asm: Micropruina sp. isolate AMR_MDS_2195
            NODE_9219_length_4074_cov_4.499129, whole genome shotgun sequence.
ACCESSION   DAOSUG010000262 DAOSUG010000000
VERSION     DAOSUG010000262.1
DBLINK      BioProject: PRJNA925477
            BioSample: SAMN32864262
            Sequence Read Archive: SRR4236673
KEYWORDS    WGS; ENV; Metagenome Assembled Genome; MAG; Third Party Data; TPA;
            TPA:assembly.
SOURCE      Micropruina sp. (wastewater metagenome)
  ORGANISM  Micropruina sp.
            Bacteria; Bacillati; Actinomycetota; Actinomycetes;
            Propionibacteriales; Nocardioidaceae; Micropruina.
REFERENCE   1  (bases 1 to 4074)
  AUTHORS   Abdulkadir,N., Saraiva,J.P., Zhang,J., Stolte,S., Gillor,O.,
            Harms,H. and Rocha,U.
  TITLE     Genome-centric analyses of 165 metagenomes show that mobile genetic
            elements are crucial for the transmission of antimicrobial
            resistance genes to pathogens in activated sludge and wastewater
  JOURNAL   Microbiol Spectr 12 (3), e0291823 (2024)
   PUBMED   38289113
REFERENCE   2  (bases 1 to 4074)
  AUTHORS   Abdulkadir,N.
  TITLE     Direct Submission
  JOURNAL   Submitted (24-MAR-2023) Environmental Microbiology, Helmholtz
            Center for Environmental Research -UFZ, Permoserstr 15, Leipzig
            04318, Germany
COMMENT     The annotation was added by the NCBI Prokaryotic Genome Annotation
            Pipeline (PGAP). Information about PGAP can be found here:
            https://www.ncbi.nlm.nih.gov/genome/annotation_prok/
            
            ##Genome-Assembly-Data-START##
            Assembly Method        :: SPAdes v. 3.11.1
            Genome Representation  :: Full
            Expected Final Version :: Yes
            Genome Coverage        :: Not Applicable
            Sequencing Technology  :: Illumina MiSeq
            ##Genome-Assembly-Data-END##
            
            ##Genome-Annotation-Data-START##
            Annotation Provider               :: NCBI
            Annotation Date                   :: 05/04/2023 04:57:10
            Annotation Pipeline               :: NCBI Prokaryotic Genome
                                                 Annotation Pipeline (PGAP)
            Annotation Method                 :: Best-placed reference protein
                                                 set; GeneMarkS-2+
            Annotation Software revision      :: 6.5
            Features Annotated                :: Gene; CDS; rRNA; tRNA; ncRNA
            Genes (total)                     :: 2,776
            CDSs (total)                      :: 2,750
            Genes (coding)                    :: 2,714
            CDSs (with protein)               :: 2,714
            Genes (RNA)                       :: 26
            tRNAs                             :: 24
            ncRNAs                            :: 2
            Pseudo Genes (total)              :: 36
            CDSs (without protein)            :: 36
            Pseudo Genes (ambiguous residues) :: 0 of 36
            Pseudo Genes (frameshifted)       :: 2 of 36
            Pseudo Genes (incomplete)         :: 32 of 36
            Pseudo Genes (internal stop)      :: 2 of 36
            ##Genome-Annotation-Data-END##
FEATURES             Location/Qualifiers
     source          1..4074
                     /organism="Micropruina sp."
                     /mol_type="genomic DNA"
                     /submitter_seqid="NODE_9219_length_4074_cov_4.499129"
                     /isolate="AMR_MDS_2195"
                     /isolation_source="Activated sludge of municipal
                     wastewater treatment plants"
                     /db_xref="taxon:2737536"
                     /environmental_sample
                     /geo_loc_name="Canada"
                     /lat_lon="49.25 N 123.1 W"
                     /collection_date="2015-06-16"
                     /metagenome_source="wastewater metagenome"
                     /note="metagenomic"
     gene            complement(325..1353)
                     /locus_tag="PLF56_09085"
     CDS             complement(325..1353)
                     /locus_tag="PLF56_09085"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_008359140.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="NAD(P)-dependent alcohol dehydrogenase"
                     /protein_id="HOQ53752.1"
                     /translation="MKAVRVHAYHSDPTIDDIPEPTLQGPLDVIVKIGGAGVCRTDLH
                     IINGDWQAAQNPDLPYVIGHENAGWVHAIGDGVTNVAVGDTVILHPQPSCGLCLACRA
                     GRDMQCSNAFFPGLSNNDGGMAEYLRTTARACVKLNPDTDPADVAALADAGITAYHAV
                     RKAASGLFPGTTAVVQGAGGLGHIGIQALKAITSARVIVVDRNPAALELAKQIGADEA
                     VVADGSHVEAVKDLTEGKGADVVFDFVAEQGAELDGWAMTAPAGYQYIIGYGGQFVAP
                     TLDFVAGEKSVIGNIVGTYNDLAELMVLAQAGKVTLHTQKYALTDALKALADLDAGNV
                     RGRAILVP"
     gene            1525..2463
                     /locus_tag="PLF56_09090"
     CDS             1525..2463
                     /locus_tag="PLF56_09090"
                     /inference="COORDINATES: ab initio
                     prediction:GeneMarkS-2+"
                     /note="Derived by automated computational analysis using
                     gene prediction method: GeneMarkS-2+."
                     /codon_start=1
                     /transl_table=11
                     /product="hypothetical protein"
                     /protein_id="HOQ53753.1"
                     /translation="MIVPRSWVLLMLLFGTGALAGCSVDGRIDVVSPTAVNLDFTVRG
                     EPSASCDGGIEGLSVTPGTDPGGIVTCHYAGTVNPSALGGAIVPVTTVGEYIAVVVNP
                     FGTSGQGTGSETINTVDVTLVLPGDVVEHNSGVAQGRELRLTDPNLLQVPGGFRVVAL
                     NHAGPSYWLWWTGGGLVLGAVAGWAASWLLRRRLRHARGVAQDPGSGATDSALQEGRD
                     QDHPGDQDDPSDQDDPGDQDDPGDQTGWRDAAEPAGATGMVAGEPAAPRLRTESTEQP
                     SDEPPGFSRPGPAHQSALARDAPARDPDPYLWAPED"
     gene            3038..3337
                     /locus_tag="PLF56_09095"
     CDS             3038..3337
                     /locus_tag="PLF56_09095"
                     /inference="COORDINATES: ab initio
                     prediction:GeneMarkS-2+"
                     /note="Derived by automated computational analysis using
                     gene prediction method: GeneMarkS-2+."
                     /codon_start=1
                     /transl_table=11
                     /product="hypothetical protein"
                     /protein_id="HOQ53754.1"
                     /translation="MQGTVPAEIQRQWRRHRSQRRHLQAVYNPSGHAGGVSASDRWTA
                     VTDIAVEVMFAGQVLESTGIAEGSPVRFTDVAQINQPLGLRAVGLDHPRGRGGPC"
     gene            3933..>4074
                     /gene="obgE"
                     /locus_tag="PLF56_09100"
                     /gene_synonym="cgtA"
                     /gene_synonym="obg"
                     /gene_synonym="yhbZ"
     CDS             3933..>4074
                     /gene="obgE"
                     /locus_tag="PLF56_09100"
                     /gene_synonym="cgtA"
                     /gene_synonym="obg"
                     /gene_synonym="yhbZ"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_002531006.1"
                     /note="ObgE; essential GTPase; exhibits high exchange rate
                     for GTP/GDP; associates with 50S ribosomal subunit;
                     involved in regulation of chromosomal replication; Derived
                     by automated computational analysis using gene prediction
                     method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="GTPase ObgE"
                     /protein_id="HOQ53755.1"
                     /translation="MAIPSFVDAATLTVAGGNGGHGCASIHREKFKPLGGPDGGNGGN
                     GGS"
BASE COUNT          592 a         1387 c         1423 g          672 t
ORIGIN      
        1 gaccccgtca tccggctgtg accccgtcat cccggctgtg accccgtcat cccggctgtg
       61 accccgtcat cccggctgtg acccccgtca tcccggctgt gaccccgtca tcccggcgaa
      121 cgccgggatc tccgccacag cgtgatcgtg acctgacagg acagggcagc ccgtcatccc
      181 ggcgcaggcc gggatctctg tgtgcgccga gatcccgggg caagcccggg atgacgagag
      241 ggcctgggat gacgagtggg cctgggatga cacgttggcc cggatgacaa gtcgccaacg
      301 gttcgcgcct tgatcagcgc ttcctcaggg caccaggatc gcccgcccgc ggacgttgcc
      361 cgcgtcgagg tcggccaggg ctttcagggc gtcggtcagc gcgtacttct gggtgtgcag
      421 ggtcaccttg ccggcctggg ccagtaccat cagctcggcc aggtcgttgt aggtacccac
      481 gatgttgccg atgacgctct tctcgccggc cacgaagtcc agcgtcggcg cgacgaactg
      541 gccgccgtag ccgatgatgt actggtagcc cgccggggcg gtcatcgccc aaccgtccag
      601 ctcggcaccc tgttcggcga cgaagtcgaa caccacgtcg gcgcccttgc cctcggtcag
      661 gtccttgacc gcctcgacgt ggctgccgtc cgcaaccacc gcctcgtccg caccgatctg
      721 cttggccagc tcgagcgcgg ccgggttgcg gtcgacgacg atcaccctgg cgctggtgat
      781 cgccttgagc gcctggatgc cgatgtggcc aagaccgccg gcgccctgca ccaccgcggt
      841 ggtgcccggg aacagtccgg acgccgcctt gcgcacggcg tgataggcgg tgatgcccgc
      901 atcggcgagc gccgcgacgt cggccggatc ggtatcaggg ttgagcttga cgcacgcgcg
      961 ggcggtggtg cgcaggtatt cggccatgcc gccgtcgttg ttgctcaggc ccgggaagaa
     1021 ggcgttggag cactgcatgt cacggccggc ccggcaggcc aggcacaacc cgcaactggg
     1081 ctggggatgc aggatcacgg tgtcgcccac cgccacattg gtgacaccgt caccgatggc
     1141 gtgcacccag cccgcgttct cgtgaccgat cacgtagggc aggtcgggat tctgggcggc
     1201 ctgccagtcg ccgttgatga tgtgcaggtc ggtgcggcag accccggcgc cgccgatctt
     1261 gacgatcacg tcaagcgggc cctgcagggt cggttcggga atgtcgtcga tcgtgggatc
     1321 ggaatggtag gcgtgtacgc gcacggcttt catgtgaact cctcgtcgtt gaaaagtggc
     1381 cggaaatgcc gacctgacac ccctgctgtc tacccgcgtc gcggtcgggt gtccacccct
     1441 gattctcatc cgccgacaag tcgtcccacg acctgagcat gcggcagggc ccggctccga
     1501 cagccgagcg ggtagcctgc gcgaatgatc gtccctcgtt cgtgggtgct gctgatgctg
     1561 ctgttcggca ctggtgccct ggcgggttgc agcgtcgatg gacgcatcga cgtggtttcg
     1621 ccgaccgcgg tcaacctgga cttcacggtg cgcggggagc ccagtgcctc ctgcgacggc
     1681 ggcatcgagg ggctgagcgt cacccccggc accgatcccg gtggcattgt gacctgccac
     1741 tacgcgggca cggtgaatcc gtcggcgttg gggggcgcca tcgttccggt caccacggtg
     1801 ggcgagtaca tcgcggtggt cgtcaaccct tttggcacct ccggccaggg tacgggcagc
     1861 gagactatca atactgtcga cgtgaccctg gtgctgcccg gcgatgtcgt ggagcacaac
     1921 agtggcgtgg cacaggggcg tgaactgcgg ctcaccgatc cgaacctgct gcaggtgccc
     1981 ggcgggtttc gggtcgtcgc gctcaaccat gcgggtccgt cctactggtt gtggtggacg
     2041 ggcggaggcc tggttctggg tgcggtcgcc ggctgggccg cctcgtggct gcttcgacga
     2101 cgactgcgac atgcgcgggg cgtggctcag gaccccggct cgggtgcgac cgactcggcg
     2161 ctgcaggaag ggcgcgacca ggaccatccg ggcgaccagg acgatccgag cgaccaggac
     2221 gatccgggcg accaggacga tccgggcgac cagactggct ggcgcgatgc cgccgagccg
     2281 gcgggtgcga cgggcatggt tgccggcgag ccggccgccc cccgccttcg caccgagtcg
     2341 accgaacagc cttccgacga gccgcccggc ttctcccggc ccggcccggc gcaccaatca
     2401 gcgctcgccc gcgacgcccc cgcccgcgac ccggatccgt acctctgggc acccgaggac
     2461 tgaggctccg atctgcgacc gtggcccgat ctcggccata gcaccgtgtg gtatccagaa
     2521 ccgatgcggc aactggtgcg gcgactgatc gccatggtgg gtgtggtggg cgccatggcc
     2581 tgacctgcga ggtggtgccc gcccaccggg cgaacccgga cggcacgctg agttgagcgg
     2641 caacgcaact ggcgtcacga agatttcact gtgcgcagat ataccgattt taccgctaga
     2701 ttcccccaga ggcggtttgc aacgtttcat ccgaaaactg gcggtagatt cggtatatcg
     2761 gctggtgatg atttcagccg atcgtcggtt cgctctgggc aagcaggctc tcgatctcgg
     2821 cgacgatgcc gtcggggtcg ccctgtagtc gcggcccggt gttgcgacgc acccgaaacc
     2881 cggcagccat caggcggttc tggcgtgcac ggtcggcgtg ccggcgttgc agtagtcgat
     2941 gctgcgggcc cgtcacccgc gtgctcgagg gtgcggcgcg tcgaggtcgc ccctgggccg
     3001 gccgaacacc acggtcggca ctcgaagcct ctgcctcgtg cagggcacgg tgccggccga
     3061 aatccagcgt cagtggcgtc ggcatcgctc acaacggaga cacctccagg ccgtctacaa
     3121 cccgtcaggt cacgccggtg gggtctcggc ctcggatcga tggaccgctg tcaccgacat
     3181 cgcggtcgag gtcatgtttg cgggtcaggt gcttgagagc accggcatcg ccgagggcag
     3241 cccggtccgg ttcaccgatg tggcgcagat caaccaaccg ctcggcttgc gggcggtcgg
     3301 gctcgaccat ccccggggcc gcggcgggcc ctgctgatac ccgccatcgc gttcgtggtg
     3361 ggtgcactcg gcgtctcagc gctgcgggag gagccgggcg ccggcacctg cgtgagggtt
     3421 cgggctttga acgcttcttc ggcgaagcgc cgggcacgta ctggcgattc ggagtcggac
     3481 caggagcacg cgcgattcgg ggttccccgg gagccgggaa gcctgttacc gcccgaattg
     3541 cgtgaccagg ctcgtggcgg gcaggggtgg cggctgggag cgaccgggca gcgacccacc
     3601 cgccgagggc gggggaacgt ccaccgatcc gcgacccgcg catgccacgt gggccccgcc
     3661 ggacgaaccc gactgacggg gcggtcgccc ctcccgtcag gtgacggtgc cggtgaccca
     3721 tcacccggac acgtgccata gcggcggcgc cgtgcggcga tcctcgcggt gcagcaacca
     3781 tacggaggac ccccgtcacg catcgcgctc tccccgccgg tgacgcaccg cgcacgtggc
     3841 cacggtcgat cgtgtgtgca cgccactttg ccgttgtggg acgatgaacc ccggcgcgtc
     3901 cgccgatttt cctcctcgcg agagtgtgac cgatggccat tccctccttc gtcgatgccg
     3961 caaccctgac ggttgccggt ggcaacggcg ggcacggctg tgcgtccatt caccgcgaga
     4021 agttcaagcc cctcggcggc cccgacggcg gcaacggcgg caacggcggt tcgg
//
LOCUS       DAOSUG010000263         4073 bp    DNA     linear   ENV 10-JUN-2024
DEFINITION  MAG TPA_asm: Micropruina sp. isolate AMR_MDS_2195
            NODE_9220_length_4073_cov_4.861623, whole genome shotgun sequence.
ACCESSION   DAOSUG010000263 DAOSUG010000000
VERSION     DAOSUG010000263.1
DBLINK      BioProject: PRJNA925477
            BioSample: SAMN32864262
            Sequence Read Archive: SRR4236673
KEYWORDS    WGS; ENV; Metagenome Assembled Genome; MAG; Third Party Data; TPA;
            TPA:assembly.
SOURCE      Micropruina sp. (wastewater metagenome)
  ORGANISM  Micropruina sp.
            Bacteria; Bacillati; Actinomycetota; Actinomycetes;
            Propionibacteriales; Nocardioidaceae; Micropruina.
REFERENCE   1  (bases 1 to 4073)
  AUTHORS   Abdulkadir,N., Saraiva,J.P., Zhang,J., Stolte,S., Gillor,O.,
            Harms,H. and Rocha,U.
  TITLE     Genome-centric analyses of 165 metagenomes show that mobile genetic
            elements are crucial for the transmission of antimicrobial
            resistance genes to pathogens in activated sludge and wastewater
  JOURNAL   Microbiol Spectr 12 (3), e0291823 (2024)
   PUBMED   38289113
REFERENCE   2  (bases 1 to 4073)
  AUTHORS   Abdulkadir,N.
  TITLE     Direct Submission
  JOURNAL   Submitted (24-MAR-2023) Environmental Microbiology, Helmholtz
            Center for Environmental Research -UFZ, Permoserstr 15, Leipzig
            04318, Germany
COMMENT     The annotation was added by the NCBI Prokaryotic Genome Annotation
            Pipeline (PGAP). Information about PGAP can be found here:
            https://www.ncbi.nlm.nih.gov/genome/annotation_prok/
            
            ##Genome-Assembly-Data-START##
            Assembly Method        :: SPAdes v. 3.11.1
            Genome Representation  :: Full
            Expected Final Version :: Yes
            Genome Coverage        :: Not Applicable
            Sequencing Technology  :: Illumina MiSeq
            ##Genome-Assembly-Data-END##
            
            ##Genome-Annotation-Data-START##
            Annotation Provider               :: NCBI
            Annotation Date                   :: 05/04/2023 04:57:10
            Annotation Pipeline               :: NCBI Prokaryotic Genome
                                                 Annotation Pipeline (PGAP)
            Annotation Method                 :: Best-placed reference protein
                                                 set; GeneMarkS-2+
            Annotation Software revision      :: 6.5
            Features Annotated                :: Gene; CDS; rRNA; tRNA; ncRNA
            Genes (total)                     :: 2,776
            CDSs (total)                      :: 2,750
            Genes (coding)                    :: 2,714
            CDSs (with protein)               :: 2,714
            Genes (RNA)                       :: 26
            tRNAs                             :: 24
            ncRNAs                            :: 2
            Pseudo Genes (total)              :: 36
            CDSs (without protein)            :: 36
            Pseudo Genes (ambiguous residues) :: 0 of 36
            Pseudo Genes (frameshifted)       :: 2 of 36
            Pseudo Genes (incomplete)         :: 32 of 36
            Pseudo Genes (internal stop)      :: 2 of 36
            ##Genome-Annotation-Data-END##
FEATURES             Location/Qualifiers
     source          1..4073
                     /organism="Micropruina sp."
                     /mol_type="genomic DNA"
                     /submitter_seqid="NODE_9220_length_4073_cov_4.861623"
                     /isolate="AMR_MDS_2195"
                     /isolation_source="Activated sludge of municipal
                     wastewater treatment plants"
                     /db_xref="taxon:2737536"
                     /environmental_sample
                     /geo_loc_name="Canada"
                     /lat_lon="49.25 N 123.1 W"
                     /collection_date="2015-06-16"
                     /metagenome_source="wastewater metagenome"
                     /note="metagenomic"
     gene            <1..981
                     /locus_tag="PLF56_09105"
     CDS             <1..981
                     /locus_tag="PLF56_09105"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_015069708.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="FAD-binding protein"
                     /protein_id="HOQ53756.1"
                     /translation="RPGSRRRDRQALRTGVRTTAAGLERRIKAERGVFLGGGGFDHNR
                     AWREQFQGVDGRASSGSKGNVGSAIEVAQRVGAAVDLMDDAWWGGSIPPATPEHSAGF
                     LVSERSMPFSIIVDPAGRRFANESESYVDLGHHMLEHATTVPGTCWMIADARHARRYL
                     RSYAMDPRAVKAMTEAGIMVKAPTLAELASRIGVDPAGLRATVETFNGYARSGIDQEF
                     GRGNSAYDRYYGDPLVRPNPNLGPLEKGPFTAVQVVPGDLGTKGGVVTDEYARALRED
                     GSVIEGLYAGGNNSASVMGRTYPGPGSTLGPAAVFGYLAAKHAVGVPVTA"
     gene            1400..2941
                     /gene="hutH"
                     /locus_tag="PLF56_09110"
     CDS             1400..2941
                     /gene="hutH"
                     /locus_tag="PLF56_09110"
                     /EC_number="4.3.1.3"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_011757824.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_function: GO:0004397 - histidine ammonia-lyase activity
                     [Evidence IEA];
                     GO_process: GO:0006548 - histidine catabolic process
                     [Evidence IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="histidine ammonia-lyase"
                     /protein_id="HOQ53757.1"
                     /translation="MQVTNVAVGMGPLSREQVIAVARHDARVTIAPDALAALAATRAR
                     IEELAVADRPVYGVSTGFGALATRHIPLERRADLQRSLIRSHAAGTGPEVEREVVRAM
                     MLLRLSTLATGRTGVRPIVAETYAALLNAGITPIVHEYGSLGCSGDLAPLAHCALAVM
                     GEGFVRDGTGERLPSADALREAGVAPVTFAEKEGLALINGTDGMLGQLVLAAHDLKRL
                     LTTADIAAAMSVEALLGTPRVFAPDLQALRPQPGQAAGARVIAAMLDGSAIAASHAEQ
                     DCPIVQDAYSLRCAPQVSGAARDTLAHCCWVAENELASAIDNPVVTLDGRVESNGNFH
                     GAPVAYALDFLAIPVADVASMSERRTDRLLDVKRSHGLPPFLADDPGVDSGHMILQYS
                     QAGIVSELKRLAAPASVDSIPSSAMQEDHVSMGWAAARKLRRALDGLTRVLAGELLSA
                     ARALDLREQRPAPGTAAVRDRIRAVVEGPGPDRFIAPELEAVVELVRGGAVVAAAEGV
                     VGVLE"
     gene            complement(3033..3806)
                     /locus_tag="PLF56_09115"
     CDS             complement(3033..3806)
                     /locus_tag="PLF56_09115"
                     /inference="COORDINATES: protein motif:HMM:NF013757.2"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_function: GO:0003677 - DNA binding [Evidence IEA];
                     GO_function: GO:0003700 - DNA-binding transcription factor
                     activity [Evidence IEA];
                     GO_process: GO:0006355 - regulation of DNA-templated
                     transcription [Evidence IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="IclR family transcriptional regulator"
                     /protein_id="HOQ53758.1"
                     /translation="MPSESSTSPAVGRALDMLTYLAQRTGTVPASTIARDLDLPRSSV
                     YHILTVLTERGFTVYVPEARGYALGAAAYQLSSAFTLHDQLERLARPVLRTVAAAAGI
                     TVHLGILRGASTMYLLKQLPPTHSELEPPLVTAVGVLLPAHLTANGRAILSQLPIEQL
                     RALYSKPGDFVSRTGRGPRSLGELQTALALERAQGWSEEVELIAAGLRSAGAPIFDLH
                     GQPVAALSCTWRARVMLREPSELIGQLISGAGEITRRLQ"
BASE COUNT          584 a         1377 c         1479 g          633 t
ORIGIN      
        1 cggccaggat ctcgtcgtcg agatcggcaa gccctgcgca ccggcgtgcg taccaccgca
       61 gccggcctgg agcggcggat caaggccgaa cgcggcgtct ttctcggcgg cggtggcttc
      121 gaccacaacc gggcgtggcg cgagcagttc cagggcgttg acggacgggc gtcgtccggc
      181 tccaagggca atgtgggctc ggcgatcgag gtggcgcagc gggtcggtgc cgcggtcgac
      241 ctgatggacg acgcctggtg gggcggctcg attccgcccg cgacgcctga gcacagcgcg
      301 ggattcctgg tcagcgagcg gtcgatgccg ttctcgatca tcgtcgatcc ggcgggccgg
      361 cggttcgcca acgaatccga gtcctacgtc gacctgggtc accacatgct cgaacacgcc
      421 acgactgtgc cgggcacctg ctggatgatc gcggacgctc ggcacgcccg ccgttacctg
      481 cgttcgtacg cgatggatcc gcgggccgtc aaggcgatga ccgaggccgg catcatggtg
      541 aaggcgccca cgctggccga actcgcgtcc aggatcggcg tggacccggc cgggttgcgg
      601 gccaccgtcg agacgttcaa cggctatgcc cgctcgggta tcgaccagga gttcggacgg
      661 ggcaactcgg cctacgaccg gtactacggc gacccactgg tgcggccgaa cccgaacctt
      721 ggcccactcg agaagggacc gttcacggcc gtgcaggtgg tgccgggcga tctgggcacc
      781 aagggtggtg tcgtgaccga cgagtatgcc cgggcgctac gtgaggatgg ctcggtgatc
      841 gagggtctct acgccggcgg caacaactcg gcgtcggtga tgggccgcac atatccgggt
      901 ccgggctcga cgctcgggcc ggccgcggtg ttcgggtacc tggccgcaaa gcatgccgtg
      961 ggggtgcccg tcacggcgta gacccggatc caccgatccg tgcctactgc gtagctacat
     1021 ccgggcggcc ctggctgcgt cggccacgct cgacagacgt ccggtacccc cgatccgacc
     1081 gcgtgacaag gccatcggtc gggtgccgtt cgcgacggcg ctcagcggtg gatcccagac
     1141 gtccgccaag gaacaggtcg agctggccgg gtccttcgcg ggccggggtg tcgacgcaac
     1201 cgacggattg cgcattcttc agcgcactcc ctcagcgttc gcggggtcga cgtcctgacg
     1261 gtcgcacgaa ctgccagggt tgctgacccc gcgacggcgt ggtcggcctc cgacgactct
     1321 gttcggatcc gagacatcag tggtgccggc ctggattccg ccccgccgac gcgccacggt
     1381 gtgggcgaga ggagtgcgca tgcaggtcac gaatgtcgcc gtggggatgg gtccgctgag
     1441 tcgggagcag gtgattgctg tcgcccgcca cgatgctcgg gtcaccatcg ccccggacgc
     1501 gttggcggcc ctggccgcca ctcgggcccg gatcgaggaa ctcgcggtcg cggaccgtcc
     1561 ggtctatggg gtctcgacgg ggttcggtgc actggccacg cggcacattc cgttggaacg
     1621 ccgcgccgac ctgcagcgca gcctgatccg ctcgcacgcc gccggcaccg ggcccgaggt
     1681 cgagcgcgag gtcgtccggg cgatgatgct gttgcgcctg tcgaccctgg ccaccggacg
     1741 aaccggcgtg cgtccgatcg tcgccgagac ctacgccgcg ctgctgaacg ccggtatcac
     1801 gccgatcgtg cacgagtacg ggtcgctggg ttgttcgggt gacctggcac ctttggcgca
     1861 ctgtgcgctg gcggtgatgg gggaggggtt cgtccgcgac gggaccggtg agcggcttcc
     1921 gtcggccgac gcactgcgtg aagccggcgt cgccccggtc accttcgccg agaaggaggg
     1981 tctggcgctg atcaacggca ccgacggcat gctcgggcaa ctcgtgttgg ctgcgcacga
     2041 cctgaagcgt ctgctgacca ccgccgacat cgccgccgcg atgagcgtcg aggcgctgct
     2101 gggcacccct cgggtgttcg cgcccgacct gcaggcgctg cgtccgcaac ccggccaggc
     2161 cgcgggcgca cgggtgatcg cggcgatgct ggacgggtcc gcgatcgcgg ccagccatgc
     2221 cgagcaggac tgcccgatcg tgcaggacgc ctattcgttg cgctgcgccc cgcaggtcag
     2281 cggcgccgcc agggacacgc tggcgcattg ctgttgggtg gccgagaacg agttggcctc
     2341 ggcgatcgac aaccccgtgg tcaccctcga cgggcgggtc gaatcgaacg gcaactttca
     2401 cggcgcgccc gtggcctacg cgctcgattt tctcgccatt ccggtcgccg acgtggcatc
     2461 catgagcgag cggcgcaccg atcgcctgct cgacgtgaag cgcagccatg ggctgccgcc
     2521 gttcctggcc gacgatcctg gtgtggattc gggccacatg atcctgcagt acagccaggc
     2581 cggcatcgtg tcggagctga agcgcctggc cgcaccggcc tcggtcgatt cgatcccgag
     2641 ttcggcgatg caggaggatc acgtgtcgat gggctgggcg gcggcgcgca aacttcgccg
     2701 tgccctggac ggactgaccc gcgtgctggc gggtgagttg ctgagcgccg cccgggcgct
     2761 cgacctgcgc gagcaacgtc cggcaccggg aacggcagcg gtgcgcgacc ggatccgtgc
     2821 ggtggtcgag ggccccggcc ccgaccgctt catcgcccca gaactggagg cggtggtcga
     2881 actggtccgc gggggcgctg tggtggcggc cgccgagggc gtggtgggcg tgttggagta
     2941 ggccgggccg ggaagctcgt gacagcgggt gggttttggc acagcagtcg gtgccgaaac
     3001 ccacccgctc ggctctggcg tggccggccc gcctattgca ggcgtcgggt gatctcaccg
     3061 gcgcccgaga tcagctggcc gatcagctcg gacggctcgc gcagcatcac ccgcgcccgc
     3121 caggtgcaac tcagggccgc caccggctgg ccgtgcagat cgaagatcgg cgcgcccgcc
     3181 gagcgcagtc cggcggcgat gagttcgacc tcctccgacc agccctgggc gcgctcgagc
     3241 gccagcgcgg tctgcagttc gccgagggat cgagggccgc ggccggtgcg cgacacgaag
     3301 tcgccaggct tgctgtacag cgcccgcaac tgctcgatcg gcagctggga caggatcgcc
     3361 cgtccgttcg cggtcagatg ggccggcagc agcacgccca cggcggtcac caggggtggt
     3421 tcgagttcac tgtgcgtggg gggcagttgc ttcagcaggt acatcgtgct cgcgccgcgc
     3481 agaatgccca ggtgcaccgt gatgccggcc gccgcggcca ccgtgcgcag caccggacgg
     3541 gccagccgct ccagttggtc gtgcagggtg aatgcggacg acagctggta ggccgccgct
     3601 cccagcgcat aacccctggc ctcgggcaca tagacggtga atccgcgctc ggtcagcacg
     3661 gtgaggatgt ggtagaccga cgagcgcggc aggtcgaggt cgcgagcgat cgtcgaggcg
     3721 ggcacggttc cggtgcgttg cgccaggtag gtcagcatgt cgagcgcccg gccgaccgcc
     3781 ggagaggtgc tcgattctga gggcattcat gctccgtctc gctcgatcgg cctgcgtctg
     3841 agtcctgacc tcgccggggc tttgttcatg tctcggatcc cagattgtag cgacccgacg
     3901 catgaaccgc cgccaccacc gtccgatcct gaggttgcag agagccgagc agaggagcat
     3961 ccatgaccat caccgccgat tcgggcctca gtaccgcgac tacgtccggc ccccggcccg
     4021 tccgcgcccc ccgcggcctc gacctgcaca ccaagggttg gccgcaggag gcc
//
LOCUS       DAOSUG010000264         4072 bp    DNA     linear   ENV 10-JUN-2024
DEFINITION  MAG TPA_asm: Micropruina sp. isolate AMR_MDS_2195
            NODE_9222_length_4072_cov_4.069206, whole genome shotgun sequence.
ACCESSION   DAOSUG010000264 DAOSUG010000000
VERSION     DAOSUG010000264.1
DBLINK      BioProject: PRJNA925477
            BioSample: SAMN32864262
            Sequence Read Archive: SRR4236673
KEYWORDS    WGS; ENV; Metagenome Assembled Genome; MAG; Third Party Data; TPA;
            TPA:assembly.
SOURCE      Micropruina sp. (wastewater metagenome)
  ORGANISM  Micropruina sp.
            Bacteria; Bacillati; Actinomycetota; Actinomycetes;
            Propionibacteriales; Nocardioidaceae; Micropruina.
REFERENCE   1  (bases 1 to 4072)
  AUTHORS   Abdulkadir,N., Saraiva,J.P., Zhang,J., Stolte,S., Gillor,O.,
            Harms,H. and Rocha,U.
  TITLE     Genome-centric analyses of 165 metagenomes show that mobile genetic
            elements are crucial for the transmission of antimicrobial
            resistance genes to pathogens in activated sludge and wastewater
  JOURNAL   Microbiol Spectr 12 (3), e0291823 (2024)
   PUBMED   38289113
REFERENCE   2  (bases 1 to 4072)
  AUTHORS   Abdulkadir,N.
  TITLE     Direct Submission
  JOURNAL   Submitted (24-MAR-2023) Environmental Microbiology, Helmholtz
            Center for Environmental Research -UFZ, Permoserstr 15, Leipzig
            04318, Germany
COMMENT     The annotation was added by the NCBI Prokaryotic Genome Annotation
            Pipeline (PGAP). Information about PGAP can be found here:
            https://www.ncbi.nlm.nih.gov/genome/annotation_prok/
            
            ##Genome-Assembly-Data-START##
            Assembly Method        :: SPAdes v. 3.11.1
            Genome Representation  :: Full
            Expected Final Version :: Yes
            Genome Coverage        :: Not Applicable
            Sequencing Technology  :: Illumina MiSeq
            ##Genome-Assembly-Data-END##
            
            ##Genome-Annotation-Data-START##
            Annotation Provider               :: NCBI
            Annotation Date                   :: 05/04/2023 04:57:10
            Annotation Pipeline               :: NCBI Prokaryotic Genome
                                                 Annotation Pipeline (PGAP)
            Annotation Method                 :: Best-placed reference protein
                                                 set; GeneMarkS-2+
            Annotation Software revision      :: 6.5
            Features Annotated                :: Gene; CDS; rRNA; tRNA; ncRNA
            Genes (total)                     :: 2,776
            CDSs (total)                      :: 2,750
            Genes (coding)                    :: 2,714
            CDSs (with protein)               :: 2,714
            Genes (RNA)                       :: 26
            tRNAs                             :: 24
            ncRNAs                            :: 2
            Pseudo Genes (total)              :: 36
            CDSs (without protein)            :: 36
            Pseudo Genes (ambiguous residues) :: 0 of 36
            Pseudo Genes (frameshifted)       :: 2 of 36
            Pseudo Genes (incomplete)         :: 32 of 36
            Pseudo Genes (internal stop)      :: 2 of 36
            ##Genome-Annotation-Data-END##
FEATURES             Location/Qualifiers
     source          1..4072
                     /organism="Micropruina sp."
                     /mol_type="genomic DNA"
                     /submitter_seqid="NODE_9222_length_4072_cov_4.069206"
                     /isolate="AMR_MDS_2195"
                     /isolation_source="Activated sludge of municipal
                     wastewater treatment plants"
                     /db_xref="taxon:2737536"
                     /environmental_sample
                     /geo_loc_name="Canada"
                     /lat_lon="49.25 N 123.1 W"
                     /collection_date="2015-06-16"
                     /metagenome_source="wastewater metagenome"
                     /note="metagenomic"
     gene            237..2339
                     /locus_tag="PLF56_09120"
     CDS             237..2339
                     /locus_tag="PLF56_09120"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_013865533.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_function: GO:0003676 - nucleic acid binding [Evidence
                     IEA];
                     GO_function: GO:0005524 - ATP binding [Evidence IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="DEAD/DEAH box helicase"
                     /protein_id="HOQ53759.1"
                     /translation="MDHATLTAADETVVSQVIEALAGPGARLRDDQRTAVAALVEPGS
                     RVLVVQATGWGKSAVYWCATALHRAAGHGPTLVVSPLLSLMRDQVAAAAKAGLRAATI
                     NSSNVRDWSAIEAELADDRLDVVLVSPERLANPGFGARVLRQMGSRLGLLVIDEAHAV
                     SDWGHDFRPDYRRVSDVLQQLNPDTPVLATTATANERVTDDVAAQLGEATLVLRGPLA
                     RASLELAVVDRLSPIQRYAWVVDHLPRLPGSGIVYVLTVADAERLAQAVQVVHGDAVP
                     VAAYTGQLDAEVRHRLEDDLQANRIKALIATSALGMGYDKPDLGFVVHVGSPPSPVSY
                     YQQVGRAGRAIDHASVVLLPSPADEGVWDYFATATIPVEPEVRRLLAALEQADGPASV
                     PQLEAETGLRRTKVDLYLKQLAVAGVTERTPDGWLATGQPWVFDAAHYEGVLAVRRRE
                     AAIMRAYTRGERCLMQLLQESLDDATAAPCGRCSVCRNTLPAPLEPEPRTDTVRAVAT
                     GLRGSAQQLDPRKMWPGGEFGARGRIPQGLLADVGRVLVHADAPEWADTLGAAAGGDE
                     AALGEVADGCVGVLSRWRADWPARPQMAVALETGGYPGLAEHVASHLARVGRLELGRL
                     AVAGDAPPPDREVPSDREAAYWRGAIEVPDAAAVAGRVVLLVVDATRSQWAVAVAAAK
                     LREAGAAMVLPLVIHRQV"
     gene            complement(2520..3206)
                     /locus_tag="PLF56_09125"
     CDS             complement(2520..3206)
                     /locus_tag="PLF56_09125"
                     /EC_number="2.-.-.-"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_011756694.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="GNAT family protein"
                     /protein_id="HOQ53760.1"
                     /translation="MSAPISLATAYPVLGLRVTAGPIELSGMDDDTLIVLANLAFEGV
                     HAPDAMPFLFPWTLAPADRFHRGFLQYHWGARAGFGPERWALELAVRHEGELVGIQGV
                     TTEEFLLTRTGETGSWLSARHQGRGIGTMMRQAICAFLFDHLDFAEITSAAFVDNAAS
                     NAVSRKVGYRPNGTVRKLRGRTAADPEDGRVAALENRYLLTPDDLVRGPELSVEGVAP
                     VRRLIGLDAG"
     gene            3309..3854
                     /locus_tag="PLF56_09130"
     CDS             3309..3854
                     /locus_tag="PLF56_09130"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_020573776.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_function: GO:0002161 - aminoacyl-tRNA editing activity
                     [Evidence IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="YbaK/EbsC family protein"
                     /protein_id="HOQ53761.1"
                     /translation="MTDPTIFGTLDAQPASARPDLLAPPVAAAIHLIPDALVAEIDPA
                     YADTEQLCAHYGVPMDASANAVIVRGTRAGVEKHACCMTLAHRRIDVNNVVRRRLDAR
                     KASFAPMDYAVEATGMEYGGINPVGLPADWPIWVDGAVAERDWVCIGSGVRHSKLFIP
                     VEGLLGLPGAERVDDLARDVG"
BASE COUNT          551 a         1412 c         1502 g          607 t
ORIGIN      
        1 cttgtgtcgc cgacactggt gtccgagacc tggtggacga agagcagcgt gtcactctgg
       61 gcgggtgagc gccatcgagc cttcgcttcg atcggcgagc tgcacgaccc gccacgtggc
      121 cgaggtgagc accgcctgag cgccaggttg ttctgcaggc ggggttatcc acagattgct
      181 gcggacggag ccgctgggtc cgttcgctgt cggaggtgcc ccctaggttg gaccgcatgg
      241 accacgccac gctcactgct gccgacgaga ccgttgtcag ccaggtcatc gaagccctgg
      301 ccgggccggg tgcgcggttg cgcgacgacc agcgcaccgc ggtcgccgcg ctggtcgaac
      361 ccggctcgcg ggtgttggtg gtgcaggcca ccgggtgggg taaatcggcc gtctactggt
      421 gtgcgacggc gctgcatcgg gctgcggggc acggccccac gctggtggtg agtccactgt
      481 tgtcgctgat gcgcgaccag gtcgcggccg ctgcgaaggc cgggcttcgg gcggccacga
      541 tcaactcctc caacgtgcgc gactggtcgg cgatcgaggc cgagctggcc gacgatcggc
      601 tcgacgtggt gctggtcagt cccgagcggt tggccaaccc gggtttcggg gcgcgggtgc
      661 tgcgtcagat gggctcgcgg ctggggctgt tggtgatcga cgaggcgcac gcggtcagcg
      721 actgggggca cgactttcgt cccgactatc ggcgggtctc cgacgtgctg cagcaactca
      781 accccgacac cccggtgctg gccaccaccg ccaccgccaa cgaacgggtc accgacgacg
      841 tcgcggccca gctgggcgag gccacgctgg tgctgcgcgg gccgttggcc cgggcgtcgc
      901 tcgaactggc ggtggtcgat cggctcagcc cgatccagcg ctacgcgtgg gtggtcgatc
      961 acctgccgcg gctgcccgga tcgggcatcg tgtacgtgct cacggtcgcc gacgccgagc
     1021 gcctggcgca ggcggtgcag gtcgtccacg gcgacgcggt gccggtggcc gcgtacaccg
     1081 gtcagctcga cgccgaggtt cggcatcgac tcgaagacga cctgcaggcc aatcgaatca
     1141 aggcgttgat cgccacctcg gccctgggca tgggctacga caagcccgac ctcggctttg
     1201 tggtgcacgt cggttcaccg ccctcaccgg tgtcgtacta ccagcaggtg ggtcgcgccg
     1261 ggcgggccat cgaccacgcc tcggtggtcc tactgccgtc cccagccgac gagggggtct
     1321 gggactactt cgccaccgcc acgattccgg tcgagcccga ggtgcggcgg ttgctggctg
     1381 cgcttgaaca ggcggacggg cccgcgtccg tgccgcagct ggaggccgag accggcctgc
     1441 gccgcaccaa ggtcgatctg tatctgaagc aactcgcggt ggcgggcgtc accgagcgaa
     1501 cccccgacgg ctggctggcg accggtcaac cctgggtctt cgatgccgcg cactacgagg
     1561 gggtgctggc cgtgcgacgc cgcgaggccg cgatcatgcg ggcctacacc cgtggcgaac
     1621 ggtgcctgat gcagctgctg caagagtcgc tggacgatgc cactgccgca ccctgcgggc
     1681 gctgctcggt gtgccgcaac acgctgccgg ccccgctgga gccggagccg cgcaccgaca
     1741 ccgtccgggc ggtggccacc gggctgcgcg gcagcgcgca gcaactcgac ccgcgcaaga
     1801 tgtggccggg tggcgagttc ggcgcgcgcg gacgcattcc gcagggcctg ctcgccgacg
     1861 tgggccgggt gctggtgcac gcagacgcgc ccgaatgggc cgacacgctg ggcgcggcag
     1921 cgggcggcga cgaggcggcg ctgggcgagg tggccgacgg ctgcgtcggt gtgctgtcgc
     1981 ggtggcgggc ggactggccg gcccgtcccc agatggcggt ggcactcgag actggcggct
     2041 accccggtct ggccgagcac gtggcgtccc acctggccag ggtcggccgg ctggagcttg
     2101 gccggttggc cgtcgcaggt gatgcgccgc ctcccgaccg tgaggtgccc agcgatcgcg
     2161 aggcggccta ctggcgcggg gcgatcgagg tgccggacgc tgccgcggtg gccgggcgcg
     2221 tcgtcctgct ggtggtggac gccacccgca gccagtgggc ggtcgcggtg gcggcggcga
     2281 agctccgcga agcgggtgcg gccatggtgc tgccgctggt gatccatcgg caggtgtgag
     2341 gtcgcgagat cccggcactc aggtcacgtg tggcgagact tcgtcggtcg agcttgtcgg
     2401 aacccatcgc gaggtcccgg cgtacaccgg gctgaccgtg cttccacacg tcatccccgg
     2461 cttgcctcgg gatctccagc cacccgaacc gcgggcccgc cggtcgaggg tgtcgaggcc
     2521 tacccggcgt ccaggccgat cagccggcgc accggtgcga ccccctcgac gctcagctcg
     2581 ggtccccgga cgaggtcgtc cggggtcagc aggtagcggt tctcgagggc cgccacccgg
     2641 ccgtcctcgg ggtcggcggc ggtgcgtccg cggagtttgc ggacggtgcc gttgggccgg
     2701 taacccacct tgcggctgac cgcgttggac gcggcgttgt cgacaaatgc ggcggaggtg
     2761 atttctgcga agtccaggtg gtcgaacagg aatgcgcaga tggcctgccg catcatcgtg
     2821 ccgatgccgc ggccctgatg ccgggcgctg agccacgagc cggtctcacc ggtgcgggtg
     2881 agcagaaact cctccgtggt gacgccttgg atgccgacca gttcgccctc gtgccggacg
     2941 gccagttcga gcgcccaccg ctccggcccg aagccggccc gcgcacccca gtggtactgc
     3001 agaaacccgc ggtggaagcg gtccgccggc gcgagcgtcc agggaaagag gaacggcatg
     3061 gcgtccggcg cgtgcacgcc ctcgaaggcc aggttggcca gcacgatcag ggtgtcgtcg
     3121 tccatgcccg acaactcgat cgggccggcg gtgacccgca ggcccagcac ggggtaggcc
     3181 gtggcgagcg agatcggcgc actcatgtcg cgacggtact actgactgct gaacagccgt
     3241 ctcgataagc ggtctcggca ggctcgacca gcggagcccg cccgtcgcgc tgcggcagga
     3301 tggacgtcat gaccgatccc accatcttcg gcaccctgga cgcgcaaccg gcgagcgccc
     3361 gtccggacct cttggcgccc ccggtggcgg cggcgatcca cctgattccg gacgcgctgg
     3421 tcgccgagat cgatccggcc tacgccgaca ccgagcagtt gtgcgcccac tacggcgtgc
     3481 cgatggatgc ctccgcgaac gccgtgatcg tgcgcggcac ccgcgccgga gtcgagaagc
     3541 acgcctgctg catgaccctg gctcaccggc ggatcgacgt gaacaacgtg gtgcggcgcc
     3601 ggctggacgc ccgcaaggcg tcgttcgcac cgatggacta cgcggtcgag gcgaccggca
     3661 tggaatacgg cggcatcaat ccagtcgggc tgccggcgga ctggccgatc tgggtggacg
     3721 gcgccgtggc cgagcgcgac tgggtgtgca tcggctcggg ggtgcggcac tccaagctgt
     3781 tcatccccgt cgagggcctg ctgggcctac ccggcgccga acgggtggac gacctcgccc
     3841 gcgacgtcgg ctgaccgctg accccggcgt ccctccccat ccgccggacc gtcgcgctcc
     3901 tccccaccgc tcccctgact atcttccgct cgccaacctt ggcgagcgga cgatagttag
     3961 gcgagccaat gatgccctgg cgcgagtggc ccgccgcgac ccgccgggcg ttcagacctt
     4021 cgccgccgct gcccggacca ggcccgcgaa ggcgtccacg tcggcctcgg tg
//
LOCUS       DAOSUG010000265         4071 bp    DNA     linear   ENV 10-JUN-2024
DEFINITION  MAG TPA_asm: Micropruina sp. isolate AMR_MDS_2195
            NODE_9227_length_4071_cov_4.153137, whole genome shotgun sequence.
ACCESSION   DAOSUG010000265 DAOSUG010000000
VERSION     DAOSUG010000265.1
DBLINK      BioProject: PRJNA925477
            BioSample: SAMN32864262
            Sequence Read Archive: SRR4236673
KEYWORDS    WGS; ENV; Metagenome Assembled Genome; MAG; Third Party Data; TPA;
            TPA:assembly.
SOURCE      Micropruina sp. (wastewater metagenome)
  ORGANISM  Micropruina sp.
            Bacteria; Bacillati; Actinomycetota; Actinomycetes;
            Propionibacteriales; Nocardioidaceae; Micropruina.
REFERENCE   1  (bases 1 to 4071)
  AUTHORS   Abdulkadir,N., Saraiva,J.P., Zhang,J., Stolte,S., Gillor,O.,
            Harms,H. and Rocha,U.
  TITLE     Genome-centric analyses of 165 metagenomes show that mobile genetic
            elements are crucial for the transmission of antimicrobial
            resistance genes to pathogens in activated sludge and wastewater
  JOURNAL   Microbiol Spectr 12 (3), e0291823 (2024)
   PUBMED   38289113
REFERENCE   2  (bases 1 to 4071)
  AUTHORS   Abdulkadir,N.
  TITLE     Direct Submission
  JOURNAL   Submitted (24-MAR-2023) Environmental Microbiology, Helmholtz
            Center for Environmental Research -UFZ, Permoserstr 15, Leipzig
            04318, Germany
COMMENT     The annotation was added by the NCBI Prokaryotic Genome Annotation
            Pipeline (PGAP). Information about PGAP can be found here:
            https://www.ncbi.nlm.nih.gov/genome/annotation_prok/
            
            ##Genome-Assembly-Data-START##
            Assembly Method        :: SPAdes v. 3.11.1
            Genome Representation  :: Full
            Expected Final Version :: Yes
            Genome Coverage        :: Not Applicable
            Sequencing Technology  :: Illumina MiSeq
            ##Genome-Assembly-Data-END##
            
            ##Genome-Annotation-Data-START##
            Annotation Provider               :: NCBI
            Annotation Date                   :: 05/04/2023 04:57:10
            Annotation Pipeline               :: NCBI Prokaryotic Genome
                                                 Annotation Pipeline (PGAP)
            Annotation Method                 :: Best-placed reference protein
                                                 set; GeneMarkS-2+
            Annotation Software revision      :: 6.5
            Features Annotated                :: Gene; CDS; rRNA; tRNA; ncRNA
            Genes (total)                     :: 2,776
            CDSs (total)                      :: 2,750
            Genes (coding)                    :: 2,714
            CDSs (with protein)               :: 2,714
            Genes (RNA)                       :: 26
            tRNAs                             :: 24
            ncRNAs                            :: 2
            Pseudo Genes (total)              :: 36
            CDSs (without protein)            :: 36
            Pseudo Genes (ambiguous residues) :: 0 of 36
            Pseudo Genes (frameshifted)       :: 2 of 36
            Pseudo Genes (incomplete)         :: 32 of 36
            Pseudo Genes (internal stop)      :: 2 of 36
            ##Genome-Annotation-Data-END##
FEATURES             Location/Qualifiers
     source          1..4071
                     /organism="Micropruina sp."
                     /mol_type="genomic DNA"
                     /submitter_seqid="NODE_9227_length_4071_cov_4.153137"
                     /isolate="AMR_MDS_2195"
                     /isolation_source="Activated sludge of municipal
                     wastewater treatment plants"
                     /db_xref="taxon:2737536"
                     /environmental_sample
                     /geo_loc_name="Canada"
                     /lat_lon="49.25 N 123.1 W"
                     /collection_date="2015-06-16"
                     /metagenome_source="wastewater metagenome"
                     /note="metagenomic"
     gene            <1..1489
                     /locus_tag="PLF56_09135"
     CDS             <1..1489
                     /locus_tag="PLF56_09135"
                     /inference="COORDINATES: ab initio
                     prediction:GeneMarkS-2+"
                     /note="Derived by automated computational analysis using
                     gene prediction method: GeneMarkS-2+.
                     GO_function: GO:0016491 - oxidoreductase activity
                     [Evidence IEA]"
                     /codon_start=2
                     /transl_table=11
                     /product="NAD(P)/FAD-dependent oxidoreductase"
                     /protein_id="HOQ53762.1"
                     /translation="QPDVGGAVRSAEVIEPGFVTDLFSAFYPLAAASSVIRGLELERH
                     GLRWVQAPTVLAHQLDDGRAAVLHRDPAATAAALDEWAPGDGQAWLDMFAHWQLIRDD
                     LLGALFTPFPPVRSGLGLLRRLKVSGALRLARLAATPVRRLAEERFAGDGGRLLLSGN
                     AMHADIPVDAAGSGLFGWLLCMLGQDVGFPVPQGGSGMLAAALRSRAESFGAQVHTGV
                     TVEAVLVQGSRATGVRLADGSVVRAERAVLADVTAPSLYSHLLADVPLPSQLRRDLRD
                     FHWDPATLKVNWALRAPIPWLAEAARGAGTVHLGVDSDGFVDFGADLSVGRLPRRPFA
                     LFGQMAVADPTRAPEGAETAWAYTHLPRRLASDPEAIATQVRRLQSAIERAAPGFGDL
                     VIGRHVQSPSDLQGSNASLSLGALNSGTAAIHQELIFRPTVGLGRPETPIPGLFLASA
                     SAHPGGGVHGACGWNAARCALAADGRLAGVRNALVRTAWERVLQG"
     gene            complement(1578..2369)
                     /locus_tag="PLF56_09140"
     CDS             complement(1578..2369)
                     /locus_tag="PLF56_09140"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_016670258.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_function: GO:0030170 - pyridoxal phosphate binding
                     [Evidence IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="YggS family pyridoxal phosphate-dependent
                     enzyme"
                     /protein_id="HOQ53763.1"
                     /translation="MSFAAEPPQSPPATYPAAIAVEDFARTLATVQARIAAAAERAGR
                     SPAQVRLLPVSKTVGEDRLRNAVAAGCHQLGENKVQEARRKYENLRDLNVAWSVIGHL
                     QTNKARDVAAFAAEFQALDSLRLAEALDRRLQAAGRALDVFVQVNTSAEDSKYGLPPD
                     QLTAFLKALTSFGALRVRGLMTLALFSSDLDRVRACFVRLRTLRDQARDTDPDSIGPG
                     ELSMGMSGDFEVAIEEGATCVRVGQAIFGSRQPRYDDQHYWPGGQ"
     gene            complement(2586..3593)
                     /locus_tag="PLF56_09145"
     CDS             complement(2586..3593)
                     /locus_tag="PLF56_09145"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_011754202.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="DNA topoisomerase IB"
                     /protein_id="HOQ53764.1"
                     /translation="MPRLRRVSPTSTGWTRRPHGKGFRYLDERGNALPAADVERIKAL
                     VIPPAWRDVWICPAPNGHLQATGVDAAGRRQYLYHPHWRVLRDKLKFDRVATAALCLP
                     DARAQVSADLARDGMPLERAAATAVRLLDLGYFRIGNDAYADANGSFGLTTLERRHVR
                     RHGRGLAFQFVGKSGIEHRVLIENEPVIEALQVMRTRRGGDRLLAYRQGNEWVDLASG
                     TVNAYLSELFGGDFTAKDFRTWHATVIFAATLAAAPPAATKTARAKVERAAVVEVASY
                     LGNTPTVAKNSYIDPRVTDLYESGATIADVAGRRYRSPASRQKALEEAVLHLLGVQPP
                     T"
     gene            complement(3630..>4071)
                     /locus_tag="PLF56_09150"
     CDS             complement(3630..>4071)
                     /locus_tag="PLF56_09150"
                     /inference="COORDINATES: protein motif:HMM:NF039409.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=2
                     /transl_table=11
                     /product="DUF6328 family protein"
                     /protein_id="HOQ53765.1"
                     /translation="RNWGELLQELRVTQTGIQILTGFLLILPFQARFAELAPVLIGVI
                     LAAVAFSALATALVLTPVTAHRILFRRHEKDVLVNIGGVMAMAGLSCLAITVGLVATL
                     VVGMIAGVGAGILAGVCALLVFALLWLVTPLMLVRRRHGAHPYE"
BASE COUNT          552 a         1410 c         1527 g          582 t
ORIGIN      
        1 ccagcccgac gtgggcggcg cggtgcgcag cgccgaggtg atcgagcccg gcttcgtcac
       61 cgacctgttc agcgcgttct acccgctggc cgcggccagc tcggtgattc gcgggctcga
      121 gttggaacgc cacggcctgc ggtgggtgca ggcgcccacc gtgctggccc atcaactgga
      181 cgacggacgg gccgcggtgc tgcaccgtga cccggccgcg accgccgccg cactggacga
      241 atgggcgccc ggtgacgggc aggcctggct ggacatgttc gcgcactggc agctgattcg
      301 cgacgacctg ctcggagccc tgttcacgcc gttcccgccg gtgcgctccg gcctcgggct
      361 gctgcgccgg ctgaaggtgt cgggcgccct gcggctggcc cggctggccg cgacgcccgt
      421 ccggcgcctg gctgaggagc gcttcgccgg tgacggtggc cggctgctgc tcagcggtaa
      481 cgccatgcat gccgacattc cggtggacgc cgccggcagc ggcctgttcg gctggctgct
      541 gtgcatgctt gggcaagacg tcggctttcc cgtgccacaa gggggttcgg gcatgctggc
      601 cgcggccctg cggtcgcggg ccgaatcgtt cggcgcgcag gtgcacacgg gcgtgacggt
      661 cgaggcggtg ctggtgcagg ggtcgcgcgc caccggcgtc cggctcgcgg acggctcggt
      721 ggtcagggct gagcgggcgg tgctggccga tgtgaccgcg ccgtcgctgt actcgcacct
      781 gttggccgac gtgccgctgc cgtcgcagtt gcgtcgcgac ctgcgcgact ttcactggga
      841 ccccgccacg ctcaaggtga actgggcgct gcgggcaccg atcccgtggc tggccgaggc
      901 ggcgcgcggt gccggcacgg tgcacctggg ggtcgattcg gacggcttcg tcgacttcgg
      961 cgccgacctg agcgtcggac ggctgccgcg ccgcccgttc gcgctgttcg gccagatggc
     1021 ggtcgccgac cccacccggg cacccgaagg cgccgagacg gcctgggcct acacgcacct
     1081 accgcggcga ctcgcgagcg accccgaggc gatcgccacg caggtgcggc ggctgcagtc
     1141 cgcgatcgag cgggccgcgc cgggcttcgg cgacctggtg atcggccggc acgtgcagtc
     1201 gccgtccgac ctgcagggca gcaacgccag cctgtcgctc ggggcgctga actcgggcac
     1261 ggcggcgatc catcaggagc tgatctttcg cccgacggtg ggcctggggc gccccgaaac
     1321 cccgatcccc gggctgttcc tggccagtgc gtccgcccac ccgggtggcg gggtgcacgg
     1381 ggcctgcggc tggaacgccg cccgctgtgc cctggcggcc gatggacgcc tcgcgggcgt
     1441 gcgcaacgcg ctggtccgca ccgcctggga gcgggtgctg cagggctgat cggtcggcgg
     1501 gatgcccgga tccgggttga ggtgacggct ccgcccggac cggatgccgg gccgggtggg
     1561 tcgacgcgga cctgggccta ctgaccgccg ggccagtagt gctgatcgtc gtagcggggc
     1621 tgtcggctgc cgaagatcgc ctggccgacc cgcacgcacg tcgcgccctc ttcgatggcg
     1681 acctcgaagt cacccgacat gcccatcgac agctcacccg gcccgatcga gtcgggatcg
     1741 gtgtcgcggg cctggtcgcg cagcgtccgc agccgcacga agcaggcccg gacccggtcg
     1801 aggtcgctgc tgaacagcgc caacgtcatc aggccgcgga cgcgcagcgc gccgaaactg
     1861 gtcagcgcct tcagaaaggc ggttagctgg tcggggggca gtccgtactt cgaatcctcg
     1921 gccgaggtgt tgacctgcac gaagacgtcc agagcgcgcc cggcggcctg cagccggcgg
     1981 tccagggctt cggccagccg caggctgtcg agggcctgga actcggcggc gaaggctgcg
     2041 acgtcgcggg ccttgttggt ctgcaggtgc ccgatcaccg accacgcgac gttcaggtcg
     2101 cgcaggttct cgtacttgcg cctggcctcc tgcaccttgt tctcgccgag ttggtgacaa
     2161 cccgcggcga cggcgttgcg cagccggtcc tcgccgaccg tcttgctgac cggcagcagg
     2221 cgaacctggg cgggggaccg tccggcccgt tcggctgcgg cggcgatgcg ggcctgcacg
     2281 gtggccaggg tgcgggcgaa gtcctcgacg gcgatggctg cggggtacgt ggcgggtggt
     2341 gactgcggcg gttcggcggc gaaggacatg agtccacgct agcgggcgcc accgacgggt
     2401 ggggctgcgc gtgtcgccgg cgagccacga cccgggatgc cggtgagact gcggcgcgtc
     2461 atcgcgtggt gtagggctca ttgcgcacgg acgaacccgc gcaacgagac aacccgcgca
     2521 acgagcgctt gaccgcgcaa cgacagcccg ccgggcgcac gtgaccgtgg cgactgtgcc
     2581 tacagtcacg tcggcggctg gacgcccagc aggtgcagca ccgcctcctc gagcgccttc
     2641 tggcgcgagg cgggggagcg gtaccgtcgt ccggcgacgt cggcgatggt ggcgccggac
     2701 tcgtacaggt cggtcacgcg cgggtcgatg taggagttct tcgccacggt cggggtgttg
     2761 ccgaggtagg acgccacctc gaccaccgcc gcgcgctcga ccttggcccg ggcggtcttg
     2821 gtggcggcgg ggggtgctgc agcgagggtg gcggcgaaga tcaccgtggc gtgccaggtg
     2881 cgaaaatcct tggcggtgaa gtcgccgccg aacagttcgg acaggtaggc gttgacggtg
     2941 ccggaggcca ggtcgaccca ctcgttgccc tggcggtagg ccagcagccg atcgccaccg
     3001 cgccgggtcc gcatcacctg cagggcctcg atcaccggtt cgttctcgat cagcacgcgg
     3061 tgctcgatgc ccgacttgcc gacgaactgg aaagcgagcc cgcgaccgtg ccgccgcaca
     3121 tgccgccgct ccagggtggt cagcccgaat gacccgttgg cgtcggcgta ggcgtcattg
     3181 ccgatccgga agtaacccag atcgagcagc cggacggcgg tggccgcggc ccgctcgagc
     3241 ggcatgccgt cccgggcgag atcggcgctg acctgcgcgc gggcgtccgg caggcacaag
     3301 gctgcggtgg cgacccggtc gaacttgagc ttgtcgcgca gcacccgcca gtgcgggtgg
     3361 tacaggtact ggcggcgtcc ggcagcgtcc acgccggtgg cctgcagatg gccgttcggc
     3421 gccgggcaga tccagacgtc gcgccaggcc ggcgggatca ccagggcctt gatgcgctcg
     3481 acgtccgctg cgggtagggc gttgccgcgc tcgtccaggt aacgaaagcc cttgccgtgc
     3541 ggacgacgcg tccagccggt cgaggtgggt gagacccggc gcaggcgcgg catggcggtg
     3601 acgctactcc cgcgccggct ggtgccgcct cattcgtacg ggtgggcgcc gtgccgccgc
     3661 cgtacgagca tcaggggcgt gaccagccac agcagggcga agaccagcag tgcgcagacg
     3721 ccggcgagga tgcccgcgcc aacgccggcg atcatgccca ccaccagggt ggccaccagc
     3781 ccgactgtga tcgccaggca cgacagcccc gccatcgcca tcaccccgcc gatgttcacc
     3841 agcacgtcct tctcatgcct gcggaacaga atgcggtgag ccgtcaccgg cgtcagcacc
     3901 agcgcggtcg ccaacgccga gaacgcgacg gctgccagaa tgacgccgat gagcaccgga
     3961 gcaagctcgg cgaaacgggc ctgaaacggc aggatcagca ggaagccggt cagaatctga
     4021 atgccggtct gcgtcacccg cagctcctgc agcaactcgc cccagttgcg g
//
LOCUS       DAOSUG010000266         4065 bp    DNA     linear   ENV 10-JUN-2024
DEFINITION  MAG TPA_asm: Micropruina sp. isolate AMR_MDS_2195
            NODE_9247_length_4065_cov_3.871820, whole genome shotgun sequence.
ACCESSION   DAOSUG010000266 DAOSUG010000000
VERSION     DAOSUG010000266.1
DBLINK      BioProject: PRJNA925477
            BioSample: SAMN32864262
            Sequence Read Archive: SRR4236673
KEYWORDS    WGS; ENV; Metagenome Assembled Genome; MAG; Third Party Data; TPA;
            TPA:assembly.
SOURCE      Micropruina sp. (wastewater metagenome)
  ORGANISM  Micropruina sp.
            Bacteria; Bacillati; Actinomycetota; Actinomycetes;
            Propionibacteriales; Nocardioidaceae; Micropruina.
REFERENCE   1  (bases 1 to 4065)
  AUTHORS   Abdulkadir,N., Saraiva,J.P., Zhang,J., Stolte,S., Gillor,O.,
            Harms,H. and Rocha,U.
  TITLE     Genome-centric analyses of 165 metagenomes show that mobile genetic
            elements are crucial for the transmission of antimicrobial
            resistance genes to pathogens in activated sludge and wastewater
  JOURNAL   Microbiol Spectr 12 (3), e0291823 (2024)
   PUBMED   38289113
REFERENCE   2  (bases 1 to 4065)
  AUTHORS   Abdulkadir,N.
  TITLE     Direct Submission
  JOURNAL   Submitted (24-MAR-2023) Environmental Microbiology, Helmholtz
            Center for Environmental Research -UFZ, Permoserstr 15, Leipzig
            04318, Germany
COMMENT     The annotation was added by the NCBI Prokaryotic Genome Annotation
            Pipeline (PGAP). Information about PGAP can be found here:
            https://www.ncbi.nlm.nih.gov/genome/annotation_prok/
            
            ##Genome-Assembly-Data-START##
            Assembly Method        :: SPAdes v. 3.11.1
            Genome Representation  :: Full
            Expected Final Version :: Yes
            Genome Coverage        :: Not Applicable
            Sequencing Technology  :: Illumina MiSeq
            ##Genome-Assembly-Data-END##
            
            ##Genome-Annotation-Data-START##
            Annotation Provider               :: NCBI
            Annotation Date                   :: 05/04/2023 04:57:10
            Annotation Pipeline               :: NCBI Prokaryotic Genome
                                                 Annotation Pipeline (PGAP)
            Annotation Method                 :: Best-placed reference protein
                                                 set; GeneMarkS-2+
            Annotation Software revision      :: 6.5
            Features Annotated                :: Gene; CDS; rRNA; tRNA; ncRNA
            Genes (total)                     :: 2,776
            CDSs (total)                      :: 2,750
            Genes (coding)                    :: 2,714
            CDSs (with protein)               :: 2,714
            Genes (RNA)                       :: 26
            tRNAs                             :: 24
            ncRNAs                            :: 2
            Pseudo Genes (total)              :: 36
            CDSs (without protein)            :: 36
            Pseudo Genes (ambiguous residues) :: 0 of 36
            Pseudo Genes (frameshifted)       :: 2 of 36
            Pseudo Genes (incomplete)         :: 32 of 36
            Pseudo Genes (internal stop)      :: 2 of 36
            ##Genome-Annotation-Data-END##
FEATURES             Location/Qualifiers
     source          1..4065
                     /organism="Micropruina sp."
                     /mol_type="genomic DNA"
                     /submitter_seqid="NODE_9247_length_4065_cov_3.871820"
                     /isolate="AMR_MDS_2195"
                     /isolation_source="Activated sludge of municipal
                     wastewater treatment plants"
                     /db_xref="taxon:2737536"
                     /environmental_sample
                     /geo_loc_name="Canada"
                     /lat_lon="49.25 N 123.1 W"
                     /collection_date="2015-06-16"
                     /metagenome_source="wastewater metagenome"
                     /note="metagenomic"
     gene            complement(<1..308)
                     /locus_tag="PLF56_09155"
     CDS             complement(<1..308)
                     /locus_tag="PLF56_09155"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_016666970.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="PTS sorbitol transporter subunit IIB"
                     /protein_id="HOQ53766.1"
                     /translation="MSEPTTTYQTVTIRKGRGGWGAGLTLTPTDEKKVVLSVTSGGIH
                     PVAARIAELSGGEAVDGFTNHVADDRVLCAVINCGGTARIGVYPRKRIPTVDVYPGEP
                     G"
     gene            complement(305..892)
                     /locus_tag="PLF56_09160"
     CDS             complement(305..892)
                     /locus_tag="PLF56_09160"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_016668161.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="PTS glucitol/sorbitol transporter subunit IIC"
                     /protein_id="HOQ53767.1"
                     /translation="MDVITWIAENFIGLFNAAAKSFTGLVTGILPLLIVLLTAMYAIT
                     TWIGEERVTRAVQWAGKYQVTRYTIMPIIAVIMLTNPMCYSFGRFLPERQKPAFYDSA
                     VSFVHPVTAFFPHANAGEIFVWTGVSAGVLAINPGAYARLAALYFLVGIVVILIRGVV
                     TEYITKLLIKRGGHEARFAEFDAAYAAGRFQGGHE"
     gene            complement(1073..1561)
                     /locus_tag="PLF56_09165"
     CDS             complement(1073..1561)
                     /locus_tag="PLF56_09165"
                     /inference="COORDINATES: protein motif:HMM:NF012579.2"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_component: GO:1902495 - transmembrane transporter
                     complex [Evidence IEA];
                     GO_function: GO:0016301 - kinase activity [Evidence IEA];
                     GO_process: GO:0008643 - carbohydrate transport [Evidence
                     IEA];
                     GO_process: GO:0009401 - phosphoenolpyruvate-dependent
                     sugar phosphotransferase system [Evidence IEA];
                     GO_process: GO:0016310 - phosphorylation [Evidence IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="PTS sugar transporter subunit IIA"
                     /protein_id="HOQ53768.1"
                     /translation="MSGNPTVTGATALRLLDGCALHHATGDRDQVLTALAQRVVESGH
                     ALPSLAAAVLAREQAYPTGLPMPVPAAIPHTDAQHVLHPGLAVASLAEPVEFGLMGGS
                     PNESLAVRLVVLLCVTDPAVQVGGLQQVLARLGDAPAVQALADHDDPTTFDAAVQAWL
                     AP"
     gene            complement(1569..2984)
                     /locus_tag="PLF56_09170"
     CDS             complement(1569..2984)
                     /locus_tag="PLF56_09170"
                     /inference="COORDINATES: protein motif:HMM:NF015568.2"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_component: GO:0016020 - membrane [Evidence IEA];
                     GO_process: GO:0009401 - phosphoenolpyruvate-dependent
                     sugar phosphotransferase system [Evidence IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="PTS transporter subunit IIC"
                     /protein_id="HOQ53769.1"
                     /translation="MEQVLAAISSHITTLGPSVVLPILITLFGLGLGLSFSRAFRAGI
                     TIGIGFIGINLVIGLLGEQVAPAAQAMATNFGVKLSVVDVGWPSSAAIAFGSTIGAVI
                     IPVGLVVNIVLLLTGLTRTLDIDLWNYWHSALIGALVAAITGNFWLGLMCAGIHLAVI
                     LSMADITAPVAQKYFKLPDISFPHGTSAPYGILAMPLNWLFDRIPGFNKLDATPEAIQ
                     KRFGIFGETLMLGLILGLLIGVLAFPFRDPAKDVAAILTLAISLAAAMFLLPRMVAIL
                     MEGLLPISEAARKFVQKRFPGRQFYIGLDSAVAVGQPSVIATSLILVPVTLLLAVALA
                     PLGNQVLPLVDLATIPFIVAVMVAVFRGNIVRSVLGGAIMIGFGLFIATWIAPAFTQV
                     AVSSGFKLGEGQTVISSLVDGANPMTGLFMGAAALGTPGVLVLAVLCVAFVVGTKVLL
                     DRRARSQLAAQTDAADDATVG"
     gene            complement(3067..3342)
                     /locus_tag="PLF56_09175"
     CDS             complement(3067..3342)
                     /locus_tag="PLF56_09175"
                     /inference="COORDINATES: protein motif:HMM:NF014368.2"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="PTS galactitol transporter subunit IIB"
                     /protein_id="HOQ53770.1"
                     /translation="MKRVLIICGTGIATSTVVASKVRDHLQEVGLQASVDQGKVMDLL
                     RGDIEADLIVATTDVPDTVTVPVVRAIALLTGFGEDEVYRQIDSHLS"
     gene            complement(3361..>4065)
                     /locus_tag="PLF56_09180"
     CDS             complement(3361..>4065)
                     /locus_tag="PLF56_09180"
                     /inference="COORDINATES: protein motif:HMM:NF012424.2"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_function: GO:0016746 - acyltransferase activity
                     [Evidence IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="2-oxo acid dehydrogenase subunit E2"
                     /protein_id="HOQ53771.1"
                     /translation="GGQTSTSSPTQGELLGDAKSRRIRVATARVLEATALIPQFTVWR
                     SLDLTRLTAARTASLKGVSWNTILLRAYALMLREYPCLNGSWAGDGVRTNPHVGVALA
                     IDTPAGLLAPVLRDPQNLGIRALNQAIKDLAATVKSGKADPSTMGGGTGTVSNLGSFG
                     IQRFNALLTPPQATALSLGSVEVQPAFDADGAIRARTVCEAGLTVDHRVADGADAARA
                     FATMQEILEDPFLLLG"
BASE COUNT          708 a         1381 c         1378 g          598 t
ORIGIN      
        1 ccgggctcac cggggtagac gtccacggtc gggattcgct tgcgcgggta gaccccgatg
       61 cgcgcggtcc cgccgcagtt gatcacggcg cacagcaccc gatcgtccgc cacgtggttg
      121 gtgaatccgt ccacggcctc gccaccggac agttcggcga tcctcgccgc caccggatgg
      181 atgccgccgc tggtcaccga gagcaccacc ttcttctcat cggtgggggt gagggtgaga
      241 cctgcgcccc agccgccgcg gcccttgcgg atcgtcacgg tctggtaggt cgtggtcggc
      301 tcgctcattc gtggcctccc tggaagcgac ccgccgcgta ggcggcgtcg aactccgcga
      361 agcgggcctc atgaccgccg cgcttgatga gcagcttcgt gatgtactcc gtgaccacgc
      421 cgcggatcag gatgaccacg atgccgacca ggaagtacaa cgccgccagc cgggcgtagg
      481 caccggggtt gatcgccagc acaccggccg agacgccggt ccacacgaag atctcgcccg
      541 cattggcgtg cggaaagaac gccgtgaccg ggtgcacgaa cgacaccgcc gagtcgtaga
      601 acgccggctt ctgccgctcg ggcaggaacc gtccgaacga gtagcacatc gggttggtca
      661 gcatgatcac cgcgatgatg ggcatgatcg tgtagcgggt gacctggtac ttgccggccc
      721 attgcacagc gcgggtgacc cgctcttcac cgatccacgt ggtgatcgca tacatcgcgg
      781 tcagcagcac gatcagcagc ggcagaatgc cggtgaccag cccggtgaag ctcttcgccg
      841 ctgcattgaa cagaccgatg aagttctcag ctatccaggt gatgacatcc atgggatccc
      901 ctcccttcct atgagtccat gcacctaagt gcggggcccg gggtcgatcc ccggagcccg
      961 gttgtgcgac ggctgggcct gatgcccccg tcggtcttcg ctgcgcggac tcgtccgcga
     1021 agcaaatccg tgtgtgcgcc cacgggcgcc gtccggtgca gggtggcggc gctcacggtg
     1081 ccagccatgc ctgcaccgcc gcatcgaagg tggtgggatc gtcgtgatcg gccagcgcct
     1141 ggacggccgg cgcgtcgccg agccgggcga gcacctgctg cagcccgccc acctggacgg
     1201 cgggatcggt cacgcacagc agcaccacca gtcgcaccgc aaggctctca ttcggcgagc
     1261 cgcccatcag accgaactcg accggttcgg ccaggctggc caccgccagc cccgggtgca
     1321 acacatgctg ggcgtcggtg tgcggaatcg ccgccggcac cggcatcggc aaccccgtcg
     1381 ggtaggcctg ctcgcgggcc agcaccgcgg cagccaggga cggcagcgcg tgaccggact
     1441 cgaccacccg ctgcgccagc gcggtgagca cctgatcgcg gtcaccggtg gcgtggtgca
     1501 gcgcgcagcc gtccagcagc cgcagtgcgg tcgcaccggt cacggtcggg ttgcctgaca
     1561 tctcgcgctc accccaccgt cgcatcgtcc gcggcatcgg tttgtgcggc cagttggctg
     1621 cgggccctgc gatccagcag caccttggtg ccgaccacga aggccacgca cagcacggcc
     1681 agcaccagca ccccgggcgt gccaagcgcc gcggcgccca tgaacagccc ggtcatcggg
     1741 ttggcgccgt ccaccagtga cgagatcacc gtctgaccct cgcccagctt gaagcccgag
     1801 ctgaccgcga cctgcgtgaa cgccggggcg atccaggtgg cgatgaagag gccgaagccg
     1861 atcatgatcg ccccaccgag caccgaacgg acgatgttgc cgcggaacac cgcgaccatc
     1921 acggccacga tgaacggaat ggtggccagg tcgaccagcg gcagcacctg gttgcccaac
     1981 ggcgccagcg ccaccgccag cagcaacgtg accggcacca ggatcagcga ggtggcaatc
     2041 accgagggct gcccgaccgc gaccgcggag tccagaccga tgtagaactg acggcccggg
     2101 aaccgctttt gaacgaactt tcgcgctgcc tctgagatgg gcagcagacc ctccatcagg
     2161 atcgccacca tgcgcggcaa caggaacatc gccgccgcca gcgagatggc cagggtcagg
     2221 atcgcagcga cgtctttggc cggatcacgg aacgggaagg ccagcacgcc gatcagcaga
     2281 ccgaggatca ggcccagcat cagcgtctca ccgaagatgc cgaaccgctt ctggatcgcc
     2341 tcgggcgtcg cgtccagctt gttgaagccg ggaatgcggt cgaagagcca gttcagcggc
     2401 atggccagaa tgccgtaggg cgccgaggtg ccgtgcggaa acgagatgtc gggcagcttg
     2461 aagtacttct gcgccaccgg tgcggtgatg tcggccatgg agaggatcac ggccaggtga
     2521 atgccggcgc acatcaggcc cagccagaag ttgccggtga tggccgccac cagggccccg
     2581 atcagggccg agtgccagta gttccacagg tcgatgtcga gcgtgcgggt caggccggtc
     2641 agcagcagca cgatgttgac caccaggccg accggaatga tcaccgcccc gatcgtcgaa
     2701 ccgaaggcga tcgctgccga cgagggccag cccacgtcga ccaccgacag cttgacgccg
     2761 aagttggtcg ccatcgcctg ggcggccggc gcgacctgct cgccgagcag cccgatcacc
     2821 aggttgatgc cgatgaagcc gatgccgatg gtgatgccgg cccggaaagc gcgactgaac
     2881 gacaggccca ggccgaggcc gaacagggtg atcaggatcg gtagcaccac gctcggaccg
     2941 agcgtggtga tgtgggacga aatggcggcg agtacttgct ccatcattgc tctcctttgc
     3001 gcggacgggg cctggctgca gacccgctgg gacgttgcat gacgcggtcg gaccgcaccg
     3061 atcgcttcag ctgaggtggg agtcgatctg gcggtagacc tcgtcctcac cgaaaccggt
     3121 gagcagcgcg atggcacgca cgaccggcac cgtgacggtg tcgggcacat cggtggtcgc
     3181 gacgatcagg tcggcctcga tgtcaccgcg cagcaggtcc atcaccttgc cctggtcgac
     3241 ggacgcctgg aggcccacct cctgcaggtg atcgcgtacc ttcgaggcca ccaccgtcga
     3301 ggtggcgata ccggtaccgc agatgatcag tactcgcttc attgcgaact ccttcagtgc
     3361 ctagccgagc agtaggaacg ggtcttccag aatctcttgc atggtggcga acgcgcgagc
     3421 ggcgtccgcc ccgtcggcca cccgatggtc gacggtcaac ccggcctcgc agaccgtccg
     3481 ggcccggatc gccccgtcgg catcgaaggc cggctgcacc tcgaccgagc cgagggacag
     3541 cgcggtcgcc tgcggcgggg tcagcagggc gttgaaccgc tggatgccga acgagccgag
     3601 gttcgacaca gtgccggtgc caccgcccat cgtgctggga tcggccttgc ccgacttgac
     3661 cgtggcggcg aggtccttga tcgcctggtt gagggcccgg atacccaggt tctgcggatc
     3721 acgcagcacc ggtgccagca ggccggccgg ggtgtcgatc gccagcgcga ccccgacgtg
     3781 ggggttggtg cggacgccgt cgccggccca actgccgttg agacaggggt actcccgcag
     3841 catcaacgca taggcccgca gcaggatggt gttccagctg acgcccttga gtgaggcggt
     3901 tcgggcggca gtcagtcgcg tcaggtcgag ggatcgccac acggtgaact gcgggatcag
     3961 ggccgtggcc tccaacaccc gcgcggtggc cacccggatg cgccgcgact tggcgtcgcc
     4021 gagcagttcg ccctgggtgg gcgaactggt cgaggtctgg ccgcc
//
LOCUS       DAOSUG010000267         4053 bp    DNA     linear   ENV 10-JUN-2024
DEFINITION  MAG TPA_asm: Micropruina sp. isolate AMR_MDS_2195
            NODE_9273_length_4053_cov_4.128314, whole genome shotgun sequence.
ACCESSION   DAOSUG010000267 DAOSUG010000000
VERSION     DAOSUG010000267.1
DBLINK      BioProject: PRJNA925477
            BioSample: SAMN32864262
            Sequence Read Archive: SRR4236673
KEYWORDS    WGS; ENV; Metagenome Assembled Genome; MAG; Third Party Data; TPA;
            TPA:assembly.
SOURCE      Micropruina sp. (wastewater metagenome)
  ORGANISM  Micropruina sp.
            Bacteria; Bacillati; Actinomycetota; Actinomycetes;
            Propionibacteriales; Nocardioidaceae; Micropruina.
REFERENCE   1  (bases 1 to 4053)
  AUTHORS   Abdulkadir,N., Saraiva,J.P., Zhang,J., Stolte,S., Gillor,O.,
            Harms,H. and Rocha,U.
  TITLE     Genome-centric analyses of 165 metagenomes show that mobile genetic
            elements are crucial for the transmission of antimicrobial
            resistance genes to pathogens in activated sludge and wastewater
  JOURNAL   Microbiol Spectr 12 (3), e0291823 (2024)
   PUBMED   38289113
REFERENCE   2  (bases 1 to 4053)
  AUTHORS   Abdulkadir,N.
  TITLE     Direct Submission
  JOURNAL   Submitted (24-MAR-2023) Environmental Microbiology, Helmholtz
            Center for Environmental Research -UFZ, Permoserstr 15, Leipzig
            04318, Germany
COMMENT     The annotation was added by the NCBI Prokaryotic Genome Annotation
            Pipeline (PGAP). Information about PGAP can be found here:
            https://www.ncbi.nlm.nih.gov/genome/annotation_prok/
            
            ##Genome-Assembly-Data-START##
            Assembly Method        :: SPAdes v. 3.11.1
            Genome Representation  :: Full
            Expected Final Version :: Yes
            Genome Coverage        :: Not Applicable
            Sequencing Technology  :: Illumina MiSeq
            ##Genome-Assembly-Data-END##
            
            ##Genome-Annotation-Data-START##
            Annotation Provider               :: NCBI
            Annotation Date                   :: 05/04/2023 04:57:10
            Annotation Pipeline               :: NCBI Prokaryotic Genome
                                                 Annotation Pipeline (PGAP)
            Annotation Method                 :: Best-placed reference protein
                                                 set; GeneMarkS-2+
            Annotation Software revision      :: 6.5
            Features Annotated                :: Gene; CDS; rRNA; tRNA; ncRNA
            Genes (total)                     :: 2,776
            CDSs (total)                      :: 2,750
            Genes (coding)                    :: 2,714
            CDSs (with protein)               :: 2,714
            Genes (RNA)                       :: 26
            tRNAs                             :: 24
            ncRNAs                            :: 2
            Pseudo Genes (total)              :: 36
            CDSs (without protein)            :: 36
            Pseudo Genes (ambiguous residues) :: 0 of 36
            Pseudo Genes (frameshifted)       :: 2 of 36
            Pseudo Genes (incomplete)         :: 32 of 36
            Pseudo Genes (internal stop)      :: 2 of 36
            ##Genome-Annotation-Data-END##
FEATURES             Location/Qualifiers
     source          1..4053
                     /organism="Micropruina sp."
                     /mol_type="genomic DNA"
                     /submitter_seqid="NODE_9273_length_4053_cov_4.128314"
                     /isolate="AMR_MDS_2195"
                     /isolation_source="Activated sludge of municipal
                     wastewater treatment plants"
                     /db_xref="taxon:2737536"
                     /environmental_sample
                     /geo_loc_name="Canada"
                     /lat_lon="49.25 N 123.1 W"
                     /collection_date="2015-06-16"
                     /metagenome_source="wastewater metagenome"
                     /note="metagenomic"
     gene            <1..976
                     /gene="tgt"
                     /locus_tag="PLF56_09185"
     CDS             <1..976
                     /gene="tgt"
                     /locus_tag="PLF56_09185"
                     /EC_number="2.4.2.29"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_016670324.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_component: GO:0005737 - cytoplasm [Evidence IEA];
                     GO_function: GO:0008479 - queuine tRNA-ribosyltransferase
                     activity [Evidence IEA];
                     GO_process: GO:0006400 - tRNA modification [Evidence IEA]"
                     /codon_start=2
                     /transl_table=11
                     /product="tRNA guanosine(34) transglycosylase Tgt"
                     /protein_id="HOQ53772.1"
                     /translation="GAFMNWPGPTFTDSGGFQVMSLGAGFKKVLAMDTVGLADDDVIA
                     EGKTRLAHVDDDGVTFTSHLNGDRHRFTPEVSMRIQHELGADIMFAFDELTTLMNTRA
                     YQEQSVARTQAWAVRCLAAHAELTRSRADKPYQALFGVVQGAQYEDLRRRAARDLAAL
                     EVGGASFDGFGIGGALEKENLGTIVGWVTDELPDDKPRHLLGISEPDDFFAAIAAGAD
                     TFDCVMPSRVARNAALYTRDGRFNVNTAAHRRAFEPIEADCDCYTCAHYTRAYLHHLF
                     KAKEMLASTLATIHNERFTVRLVDEIRASIEGGEFDAFREESLGRFYD"
     assembly_gap    1053..1152
                     /estimated_length=100
                     /gap_type="within scaffold"
                     /linkage_evidence="paired-ends"
     gene            complement(1412..2149)
                     /locus_tag="PLF56_09190"
     CDS             complement(1412..2149)
                     /locus_tag="PLF56_09190"
                     /inference="COORDINATES: protein motif:HMM:NF037170.2"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="VOC family protein"
                     /protein_id="HOQ53773.1"
                     /translation="MDATRPFWLSAFIDLPGARFGVTTAFWAAVTGYALSPLRGRDRE
                     FGTLVPGDGDDYLRVQRLGDEVPRLHLDVHVPDPDAAADRAVALGADAVADQGFGYRV
                     LRSPGGLVFCLVRHPNGTRPPAVTWPSGHRSRVDQVALDLPRNVHPVESAFWSALLDE
                     PVRALSGDEFTAVDRRPNLPLRLLLHRLDEPTGPTRAHLDLATDDVAAEVARHVARGA
                     AHVRDHDGWTVLRDPSGLPYCVTGRDP"
     gene            2201..2446
                     /locus_tag="PLF56_09195"
     CDS             2201..2446
                     /locus_tag="PLF56_09195"
                     /inference="COORDINATES: ab initio
                     prediction:GeneMarkS-2+"
                     /note="Derived by automated computational analysis using
                     gene prediction method: GeneMarkS-2+."
                     /codon_start=1
                     /transl_table=11
                     /product="hypothetical protein"
                     /protein_id="HOQ53774.1"
                     /translation="MALSREDMIEILAEADPVGLIATGAPRDEYAAEADAILSLRGVP
                     KLTEITGVFAVSFSEPGACTRETARWIAEEMVRRAAG"
     gene            2655..3920
                     /locus_tag="PLF56_09200"
     CDS             2655..3920
                     /locus_tag="PLF56_09200"
                     /inference="COORDINATES: protein motif:HMM:NF019310.2"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_function: GO:0022857 - transmembrane transporter
                     activity [Evidence IEA];
                     GO_process: GO:0055085 - transmembrane transport [Evidence
                     IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="MFS transporter"
                     /protein_id="HOQ53775.1"
                     /translation="MAAVTNRSIGAPAAYYLFEGGAALLNALGFTLMLVLQVQVVGLS
                     PFELVLMGTVLELTLVLFEIPTGVVADVYSRRRSVLIGAAVIGVSLLVQGLWPIFWPT
                     LLAQALWGIGYTFISGAVQAWITDEVGEQAVQPVFTRGVQVALGATIVGTLLAGLLGQ
                     FDLRLPMVFAGVGYLLLAALLFVVMPETAFQPAPRADRQNWRHLTQTLRISIGLARRR
                     PLIRGFAWVGLFVGLSSEAVDRLWTARLLESFALPPLAGTQGTALWFSLFALVGTVVS
                     LVASLIANRLAARRLKQLHPGGLLAALVVLQVVGVIGFALVGSVLVALVCLWLRDAAR
                     ALAEPVQAAWLNRSIESRARATTLSLIGQVDALGQVAGGPPLGALATRTSVPVGLVAS
                     AVVLAPAAVILARLGRDAASEEPVTTAEG"
BASE COUNT          539 a         1309 c         1475 g          630 t
ORIGIN      
        1 gggcgccttc atgaactggc cggggccgac cttcaccgac tcaggcgggt ttcaggtgat
       61 gagcctcggc gccgggttca agaaggtgct ggccatggac accgtcggcc tcgccgacga
      121 cgacgtgatc gccgagggca agacccggct cgcacatgtg gacgacgacg gagtcacctt
      181 caccagccac ctcaacggcg accggcaccg gttcaccccc gaggtgagca tgcgcattca
      241 gcacgagctc ggcgccgaca tcatgttcgc cttcgacgag ttgaccaccc tgatgaacac
      301 ccgcgcctac caggagcagt cggtggcgcg cacccaggcg tgggccgtcc gctgcctggc
      361 cgcgcacgcc gaactgacca gaagccgcgc cgacaagcca taccaggcac tgttcggcgt
      421 cgtccagggc gcgcagtacg aagacctgcg ccggcgggct gcgcgcgacc tggccgccct
      481 cgaggtgggc ggcgccagct tcgacgggtt cggcatcggc ggcgcgctcg agaaggagaa
      541 cctcggcacc atcgtgggct gggtgaccga cgaactgccg gacgacaagc cccgacacct
      601 gctcggcatc tcggagccgg acgacttctt cgccgcgatc gccgccggtg ccgacacttt
      661 cgactgcgtg atgccctcgc gggtggcccg caacgccgcc ctgtacaccc gcgacggacg
      721 gttcaacgtg aacaccgctg cgcaccggcg ggcgttcgag ccgatcgagg ccgactgcga
      781 ctgctacacg tgtgcgcact acacccgggc ctacctgcat cacctgttca aggccaagga
      841 gatgctggcc tcgaccctgg ccacgatcca caacgagcgg ttcacggtgc gactggtcga
      901 tgagatccgg gcgtcgatcg agggtgggga gttcgacgcc ttccgcgagg agtctctcgg
      961 ccggttctac gactgaagcg agtcgagctg aggctgcact ggcgccggag gcccatgtgt
     1021 cctgggtggg cgtgcgaaac tgcaccagat ttnnnnnnnn nnnnnnnnnn nnnnnnnnnn
     1081 nnnnnnnnnn nnnnnnnnnn nnnnnnnnnn nnnnnnnnnn nnnnnnnnnn nnnnnnnnnn
     1141 nnnnnnnnnn nngccacctg agtgcgctcg tccggcgtcg tggctgcgaa atgtggtgca
     1201 gtttcgcccg gacgtgcggt cggcgggcta cgccgagggg gggtgcctgc cctgtcgtcg
     1261 gacggccgca cgtcctgggt gggcgtgcga aactgcacca gatttgccac ctgagtgcgc
     1321 tcgtccggcg tcgtggctgc gaaatgtggt gcagtttcgc gtgtacgtgc cgtcggcggc
     1381 cagtccggtg ggcgagtgcc tgcccctgcc cctacggatc cctaccggtg acgcagtagg
     1441 gcagcccgga cgggtcgcgc agcaccgtcc agccgtcgtg gtcgcggacg tgcgccgcgc
     1501 cccgcgcgac gtgccgcgcc acctcggccg cgacgtcgtc cgtggccagg tcgaggtggg
     1561 cgcgggtggg gcccgtcggc tcgtccaggc ggtgcagcag caggcgcagc ggcaggttgg
     1621 ggcggcgatc gacggcggtg aactcatcgc cgctgagcgc acggaccggc tcgtcgagca
     1681 gggccgacca gaacgcactc tcgacggggt ggacgttgcg gggcaggtcg agggccacct
     1741 ggtcgacgcg gctgcggtgc ccggacggcc aggtgaccgc tggcggacgg gtgccgttgg
     1801 ggtgccgcac caggcagaag accaggccac cgggggagcg cagtacccgg tagccgaagc
     1861 cctgatccgc caccgcatcc gcgccgagtg cgacagcgcg atcggcggcg gcgtccggat
     1921 cgggcacgtg cacgtccagg tgaagccgtg gaacttcatc gccgaggcgt tgcacccgca
     1981 ggtagtcgtc gccgtcgccg ggcaccaagg tgccgaactc gcggtcgcgg ccacgcaacg
     2041 gcgagagtgc atacccggtg accgcggccc agaaggcggt cgtgaccccg aaccgggcgc
     2101 ccggcaggtc gatgaatgcg ctgagccaga agggtcgggt ggcgtccatg cgtcgaaccg
     2161 tagtgcggcg agccgtccgg ggctcttcta agctcagccc atggcgctct cacgcgagga
     2221 catgatcgag attctggccg aggcggaccc ggtcgggctg atcgcgaccg gtgcgccccg
     2281 cgacgagtac gcggccgagg cggacgcgat cctgtcgctg cggggcgtgc cgaagctgac
     2341 cgagatcacc ggggtgttcg cggtcagctt cagcgagccc ggcgcctgca cccgggaaac
     2401 cgcgcggtgg atcgccgagg aaatggtgcg gcgagcggcg ggctgagcct ggatccaccg
     2461 atctgcgcct gctgcgcggc cccgtccggg ggccctgggg ttgccggatg gtgcgcggcc
     2521 acgtccgggg ggccctgggg taccggatag gcaggcagat gccgccgacc tggcattccg
     2581 agttggcgcg ctgcgggtct gcgtgaccgg gcagactgtc gtccgtcccg tggtcgggac
     2641 cgagaggacg tccgatggca gcagtgacca accggtccat cggcgcgccc gcggcctact
     2701 acctgttcga gggcggtgcc gcactgctga acgcgctcgg cttcaccctg atgctggtcc
     2761 tgcaggtgca ggtggtcggc ctgtcgccct tcgaactggt gctgatgggg acggtgctcg
     2821 aactcaccct ggtgctgttc gagattccga ccggcgtggt cgccgacgtg tactcccggc
     2881 gtcggtcggt gctgatcggt gcggcggtga tcggcgtcag cctgctggtg caggggctgt
     2941 ggccgatctt ctggccgacc ctgctggctc aggcgctgtg gggcatcggc tacacgttca
     3001 tctcgggagc agtgcaggcg tggatcaccg acgaggtggg tgagcaggcc gtgcagccgg
     3061 tgttcacccg cggcgtgcaa gtggcactgg gggccacgat cgtcggcacg ctgctcgccg
     3121 gtctgctcgg gcagttcgac ctccggttgc cgatggtctt cgccggcgtg ggctacctgc
     3181 tgctcgccgc cctgctcttt gtggtgatgc cagagactgc cttccagccc gcgccgaggg
     3241 cggatcggca gaactggcgg cacctcacgc agaccctgcg aatcagcatt gggctggccc
     3301 gacgacgtcc gctgatcagg ggtttcgcct gggtcgggct gttcgtcgga ttgtcgtccg
     3361 aggcggtcga ccggttgtgg acggcccgcc ttctggagtc gttcgcgctg ccgccgttgg
     3421 cgggcaccca gggcacggcg ctgtggttca gcctgttcgc actggtcggc acggtggtgt
     3481 cgctggtggc cagcctgatc gccaaccggc tggccgcccg ccggctcaag caactgcacc
     3541 cgggggggct actggccgcc ctggtcgtgc ttcaggtagt gggtgtgatc gggttcgccc
     3601 tggtgggctc ggtgttggtg gcgctggtct gcctgtggct ccgggacgct gcgcgtgccc
     3661 tggccgaacc cgtccaggcc gcgtggttga acaggtccat cgagtcgcgg gcccgggcca
     3721 ccacgctgtc gctgatcgga caggtggacg ccctggggca ggtcgccggc ggaccgcccc
     3781 tgggagcgct ggccacgcgc acgtccgtgc cggtcggtct ggtggcctcc gcggtggtgc
     3841 tggctcccgc ggcggtgatc ctggcccggt tgggccgcga tgcggcgtcc gaggaaccgg
     3901 ttaccactgc cgaggggtga aggagtcggg tggcgtggtt ggggacggtt ctcaataccc
     3961 gccggcggcc gaatggcggg ggttgggaac cgtccgcaat acccgccagc gaactcaggg
     4021 cgtggtctgg tcgcccggac ggccgccacc acc
//
LOCUS       DAOSUG010000268         4034 bp    DNA     linear   ENV 10-JUN-2024
DEFINITION  MAG TPA_asm: Micropruina sp. isolate AMR_MDS_2195
            NODE_9331_length_4034_cov_3.995225, whole genome shotgun sequence.
ACCESSION   DAOSUG010000268 DAOSUG010000000
VERSION     DAOSUG010000268.1
DBLINK      BioProject: PRJNA925477
            BioSample: SAMN32864262
            Sequence Read Archive: SRR4236673
KEYWORDS    WGS; ENV; Metagenome Assembled Genome; MAG; Third Party Data; TPA;
            TPA:assembly.
SOURCE      Micropruina sp. (wastewater metagenome)
  ORGANISM  Micropruina sp.
            Bacteria; Bacillati; Actinomycetota; Actinomycetes;
            Propionibacteriales; Nocardioidaceae; Micropruina.
REFERENCE   1  (bases 1 to 4034)
  AUTHORS   Abdulkadir,N., Saraiva,J.P., Zhang,J., Stolte,S., Gillor,O.,
            Harms,H. and Rocha,U.
  TITLE     Genome-centric analyses of 165 metagenomes show that mobile genetic
            elements are crucial for the transmission of antimicrobial
            resistance genes to pathogens in activated sludge and wastewater
  JOURNAL   Microbiol Spectr 12 (3), e0291823 (2024)
   PUBMED   38289113
REFERENCE   2  (bases 1 to 4034)
  AUTHORS   Abdulkadir,N.
  TITLE     Direct Submission
  JOURNAL   Submitted (24-MAR-2023) Environmental Microbiology, Helmholtz
            Center for Environmental Research -UFZ, Permoserstr 15, Leipzig
            04318, Germany
COMMENT     The annotation was added by the NCBI Prokaryotic Genome Annotation
            Pipeline (PGAP). Information about PGAP can be found here:
            https://www.ncbi.nlm.nih.gov/genome/annotation_prok/
            
            ##Genome-Assembly-Data-START##
            Assembly Method        :: SPAdes v. 3.11.1
            Genome Representation  :: Full
            Expected Final Version :: Yes
            Genome Coverage        :: Not Applicable
            Sequencing Technology  :: Illumina MiSeq
            ##Genome-Assembly-Data-END##
            
            ##Genome-Annotation-Data-START##
            Annotation Provider               :: NCBI
            Annotation Date                   :: 05/04/2023 04:57:10
            Annotation Pipeline               :: NCBI Prokaryotic Genome
                                                 Annotation Pipeline (PGAP)
            Annotation Method                 :: Best-placed reference protein
                                                 set; GeneMarkS-2+
            Annotation Software revision      :: 6.5
            Features Annotated                :: Gene; CDS; rRNA; tRNA; ncRNA
            Genes (total)                     :: 2,776
            CDSs (total)                      :: 2,750
            Genes (coding)                    :: 2,714
            CDSs (with protein)               :: 2,714
            Genes (RNA)                       :: 26
            tRNAs                             :: 24
            ncRNAs                            :: 2
            Pseudo Genes (total)              :: 36
            CDSs (without protein)            :: 36
            Pseudo Genes (ambiguous residues) :: 0 of 36
            Pseudo Genes (frameshifted)       :: 2 of 36
            Pseudo Genes (incomplete)         :: 32 of 36
            Pseudo Genes (internal stop)      :: 2 of 36
            ##Genome-Annotation-Data-END##
FEATURES             Location/Qualifiers
     source          1..4034
                     /organism="Micropruina sp."
                     /mol_type="genomic DNA"
                     /submitter_seqid="NODE_9331_length_4034_cov_3.995225"
                     /isolate="AMR_MDS_2195"
                     /isolation_source="Activated sludge of municipal
                     wastewater treatment plants"
                     /db_xref="taxon:2737536"
                     /environmental_sample
                     /geo_loc_name="Canada"
                     /lat_lon="49.25 N 123.1 W"
                     /collection_date="2015-06-16"
                     /metagenome_source="wastewater metagenome"
                     /note="metagenomic"
     gene            complement(<1..673)
                     /locus_tag="PLF56_09205"
     CDS             complement(<1..673)
                     /locus_tag="PLF56_09205"
                     /inference="COORDINATES: protein motif:HMM:NF015755.2"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="LCP family protein"
                     /protein_id="HOQ53776.1"
                     /translation="MRTRSDRLVAVATLGAFVVVAGVLGLVSLYLGAVTQAMSGVQRT
                     ASLPEYEGRPAEQRTDGNGAMRYVVLVTDENGALASAYLAQLTAARDALHLIGLPANL
                     LVRDPHGKDATLAARYQEGPDAAVRAIEGLVNVRVDHLVQVELEGFTRIIDVLGGVTV
                     DNRNQMVAEGWHFAAGEVRLTGAEAQIYLSSSTHSMTRLERTEAVFVEIVRGMASGDA
                     LTNPAK"
     gene            813..2060
                     /locus_tag="PLF56_09210"
     CDS             813..2060
                     /locus_tag="PLF56_09210"
                     /inference="COORDINATES: protein motif:HMM:NF019350.2"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_function: GO:0000155 - phosphorelay sensor kinase
                     activity [Evidence IEA];
                     GO_function: GO:0005524 - ATP binding [Evidence IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="sensor histidine kinase"
                     /protein_id="HOQ53777.1"
                     /translation="MNARELWFPPGATRHDWVLDGMLALAMFLLTVLPYASYSAWNST
                     PRMLLMFTLTLMVAPLVLRRHSPLLMLAGCTVAGIAQVIVVNTFTATLVVLPIVAYSV
                     ARWVPGVMARSVVVIGAFASVIGPLRWATAAEGVSISARGIVLFVLASFVCLGLIVTP
                     YAIGRRVRESSAFHADRVAAAEERYHLLLAEREQQTRLAESRARATIARELHDIVAHS
                     LSVMIVQAEGGRALAAKRPQAAVSALDTIADTGREALNEMRRIVGVLRSEPERKGAEW
                     EPAPSLADIPDLVHRASDRATLVVHGEPPRVSPALELTLYRVVQEALTNFLKHAGPTA
                     TAVVTIDYTPIEIMVEVVDNGPGPTQVTTTPGHGLRGMAERVSSMDGRLEAHPLALGG
                     FVVRAVLPVHHRQTSTSDRGMQR"
     gene            2057..2716
                     /locus_tag="PLF56_09215"
     CDS             2057..2716
                     /locus_tag="PLF56_09215"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_016454908.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_function: GO:0003677 - DNA binding [Evidence IEA];
                     GO_process: GO:0000160 - phosphorelay signal transduction
                     system [Evidence IEA];
                     GO_process: GO:0006355 - regulation of DNA-templated
                     transcription [Evidence IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="response regulator transcription factor"
                     /protein_id="HOQ53778.1"
                     /translation="MSQSIRVFLADDQALVRSGFRMLIESEDDLVVVGEASDGAAAVR
                     ALMAQPAEVVLMDIRMPVMDGVEATRQIVQAGLGTRVLVLTTFDLDEYVFAALKAGAS
                     GFLLKDARPADLLNAIRTVAAGEAVMAPSATRRLLDHVVPTLPTSPEADHRLDVLTDR
                     EREVLIEIAAGATNSEIATRLYMAEGTVKTHIGRLLAKLHIRDRVGLVLLAYETGLVR
                     R"
     gene            2767..>4034
                     /gene="thrC"
                     /locus_tag="PLF56_09220"
     CDS             2767..>4034
                     /gene="thrC"
                     /locus_tag="PLF56_09220"
                     /EC_number="4.2.3.1"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_016456346.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_function: GO:0004795 - threonine synthase activity
                     [Evidence IEA];
                     GO_process: GO:0009088 - threonine biosynthetic process
                     [Evidence IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="threonine synthase"
                     /protein_id="HOQ53779.1"
                     /translation="MRYLSTRSSGGPGLPFCDILLSGLAPDGGLYLPEHYPQLSAQTL
                     ADWDVLLHTQGYAAVAFEVLSLFVDDIPADDLRAICARAYAPEVFGAVVVPVTELSDD
                     LWLAHLSEGPTAAFKDMAMQLLGQLFEYELARRGEVLTILGATSGDTGSAAEYAMRGR
                     AGITVFMLSPKGRMSPFQQAQMFGLDDPRIVNLAVDGVFDDCQDLVKAVLGDAAFKAE
                     HSIGAVNSINWGRVVAQVIYYVVSYLRVTHRLRATDRPVAAVSFAVPTGNFGNICAAH
                     VARQMGLPIDTLILATNENNVLDEFFRTGVYRVRSGAAETSSPSMDISKASNFERFVF
                     DLLGRDGARTGELFGRGLGETGVIDLSVSPEFATMRARYGFVSGTSTHADRLAAIRSV
                     EATDGVVVDPHTADAIKVAHDLRAGIQGPI"
BASE COUNT          586 a         1424 c         1389 g          635 t
ORIGIN      
        1 ccttggccgg gttggtcagc gcatcgccgc tggccatgcc ccggacgatc tcgacgaaca
       61 ccgcctcggt gcgctcgagc cgggtcatcg aatgcgtgga cgaactgagg tagatctgcg
      121 cctcggcacc ggtcagccgc acctcaccgg cggcgaagtg ccagccctcc gccaccatct
      181 ggttgcggtt gtcgacagtg accccgccca gcacgtcgat gatgcgagtg aacccctcca
      241 actcgacctg caccaggtga tcgacgcgca cattcaccag gccctcgatc gcgcgaaccg
      301 cggcgtcggg cccctcttgg tagcgtgccg ccaacgtcgc atccttacca tgcggatcgc
      361 gcaccagcag gttcgccggc agaccgatca ggtgcaacgc gtcccgtgcg gcggtcagtt
      421 gggccaggta ggcactggcc agggcaccgt tctcgtcggt gaccagcacc acgtaccgca
      481 tggccccatt gccgtcggtg cgctgctcgg cgggacgtcc ctcgtactcg ggcagcgagg
      541 cggtgcgctg cacccccgac atggcctgcg tgacggcccc gagatagagc gacaccaggc
      601 ccagcacccc ggcaacgacg acgaacgcgc cgagggtggc gacggccacc aggcggtcgc
      661 tacgggtacg catgcggcca aggctagatt cccgaggcaa ccgctcgcgg caaccggctc
      721 gcgggccgtc cggtaccccc cggacgatgc ggcggcctgc gttgggttga ggcctacgct
      781 agtggcgtgt cccccacacc actaggcacg gtatgaacgc gcgcgagttg tggtttccgc
      841 ccggtgccac caggcacgac tgggtgctcg acggcatgct cgccctggcc atgtttctgc
      901 tgaccgtgct gccgtacgcg agctactcgg cctggaactc gaccccgcgc atgctgctga
      961 tgttcaccct gaccctgatg gttgcccccc tggtgctgcg ccggcattcg ccactgctga
     1021 tgctggccgg gtgcacggtg gccggcatcg cccaggtgat cgtggtcaac accttcacag
     1081 ccaccctggt ggtgctgccg atcgtggcct actcggtcgc ccgctgggtg cccggcgtga
     1141 tggcccgctc ggtggtcgtg atcggcgcgt tcgcctcggt gatcgggccg ctgcgctggg
     1201 cgaccgccgc cgagggcgtc agcatcagcg cccgcggcat cgtgctgttc gtgctggcct
     1261 ccttcgtgtg cctgggcctg atcgtcaccc cgtacgcgat cggacggcgg gtgcgcgagt
     1321 cgtccgcctt ccacgccgac cgggtggcgg ccgccgagga gcgctaccac ctgctgctgg
     1381 ccgagcgtga acagcagacc cggctggccg agtcgcgggc ccgggccacc atcgcccgcg
     1441 agctgcacga catcgtggcg cactcgctgt cggtgatgat cgtgcaggcc gagggcggac
     1501 gggcactggc cgcgaaacgt ccgcaggcgg cggtgagcgc gctcgacacg atcgccgaca
     1561 ccggccgcga ggcgctcaac gagatgcgcc gcatcgtcgg ggtgctgcgc agcgaacccg
     1621 aacgcaaggg cgccgaatgg gagccggcac cgtccctggc cgacattccc gacctggtgc
     1681 accgggcgtc cgatcgcgcc acgctggtgg tgcacggcga gccgccgcgg gtgtctcctg
     1741 cgctggagct gaccctgtac cgggtcgtcc aagaggcgct gacgaacttt ctcaagcacg
     1801 ccggcccgac cgcgaccgcc gtcgtcacca tcgactacac cccgatcgag atcatggtcg
     1861 aggtggtcga caacggcccc ggacccaccc aggtcaccac cacgcccggc cacggcctgc
     1921 gcggcatggc cgagcgggtc tcgtccatgg acggacggct cgaggcccac ccgctggcgc
     1981 tgggcgggtt cgtggtgcgt gccgtgctgc ccgtccatca ccgccagacc agcacaagcg
     2041 acaggggaat gcagcgatga gccaatcgat ccgggtgttt ctggccgacg accaggcgtt
     2101 ggtccgcagc ggattccgca tgctgatcga gtccgaggac gacctggtgg tggtcggtga
     2161 ggcatcggac ggcgccgcgg ccgtccgggc gctgatggcg caaccggctg aggtggttct
     2221 gatggacatt cgtatgccgg tgatggacgg ggtcgaggcc acccggcaga tcgtgcaggc
     2281 ggggctgggc accagggtgc tggtgctgac caccttcgac ctcgacgagt acgtgttcgc
     2341 ggcgctcaag gcgggggcgt cggggtttct gctcaaggac gcgcggccgg ccgacctgtt
     2401 gaacgcgatc cgcacggtcg cggccggcga ggcggtgatg gcgccgtccg cgaccaggcg
     2461 gctgctcgac cacgtggtgc cgaccctgcc cacctcgccc gaggccgatc accggctcga
     2521 cgtgctgacc gatcgcgagc gcgaggtgct gatcgagatc gcggcggggg ccaccaactc
     2581 cgagatcgcg acccggctgt acatggccga gggcacggtg aagacccaca tcggacggct
     2641 gctggccaag ttgcacattc gcgaccgggt cgggctggtg ctgctggcct acgagaccgg
     2701 gctcgtgcgc cgctgacgtc ctttccacgt gaaaggggcc gcggacgggt tcggtaggct
     2761 cggcgggtgc gttacctgtc gacccgcagc tccgggggcc ccggcctgcc gttctgcgac
     2821 attctgctca gcggtctggc cccggacggc ggcctctacc tgcccgagca ctacccgcag
     2881 ctgagcgcac agaccctcgc cgattgggac gtcctgttgc acacgcaggg ttacgccgcc
     2941 gtcgcgttcg aggtgctcag cctcttcgtg gacgacatcc cggccgatga cctacgcgcc
     3001 atctgcgccc gcgcctacgc ccccgaggtg ttcggcgccg tggtggtgcc cgtcaccgaa
     3061 ctctcggacg acctgtggct ggcccacctg tccgagggtc cgacggctgc gttcaaggac
     3121 atggccatgc agctgctcgg ccagctgttc gagtacgagt tggcccggcg cggcgaggtg
     3181 ctcaccattc tcggcgccac gtccggcgac accggctcgg ccgccgagta cgccatgcgc
     3241 ggacgcgccg gcatcacggt cttcatgctc tcgcccaagg ggcggatgag cccctttcag
     3301 caggcgcaga tgttcggcct ggacgatccg cgcatcgtca atctcgccgt cgacggggtc
     3361 ttcgacgact gccaggacct ggtcaaggcc gtgcttggcg atgcggcctt caaggccgag
     3421 cacagcatcg gcgcggtcaa ctcgatcaac tggggccggg tggttgccca ggtgatctac
     3481 tacgtggtca gctacctgcg ggtcacgcat cggctgcgag ccaccgaccg tccggtcgcc
     3541 gcggtgtcgt tcgccgtacc gaccggcaac ttcggcaaca tctgcgctgc gcacgtggcc
     3601 cggcagatgg ggctgcccat cgacaccctg atcctggcca ccaacgagaa caacgtgctc
     3661 gacgagttct ttcgcaccgg cgtctaccgg gtgcgctcgg gggcggccga gacgtccagc
     3721 ccgtcgatgg acatcagcaa ggccagcaac ttcgaacggt tcgtgttcga cctgctgggc
     3781 cgcgacggcg cccgcaccgg tgagctgttc ggccgcgggc tcggcgagac cggggtgatc
     3841 gacctctcgg tcagccccga gttcgccacc atgcgcgcgc gctacgggtt cgtgtcgggc
     3901 acctcgaccc atgccgaccg gctcgccgcc attcgcagcg tcgaggccac ggacggggtg
     3961 gtggtcgacc cgcacaccgc cgacgcgatc aaggtggcgc acgacctgcg ggcaggaatc
     4021 cagggaccga tcat
//
LOCUS       DAOSUG010000269         4018 bp    DNA     linear   ENV 10-JUN-2024
DEFINITION  MAG TPA_asm: Micropruina sp. isolate AMR_MDS_2195
            NODE_9367_length_4018_cov_4.131971, whole genome shotgun sequence.
ACCESSION   DAOSUG010000269 DAOSUG010000000
VERSION     DAOSUG010000269.1
DBLINK      BioProject: PRJNA925477
            BioSample: SAMN32864262
            Sequence Read Archive: SRR4236673
KEYWORDS    WGS; ENV; Metagenome Assembled Genome; MAG; Third Party Data; TPA;
            TPA:assembly.
SOURCE      Micropruina sp. (wastewater metagenome)
  ORGANISM  Micropruina sp.
            Bacteria; Bacillati; Actinomycetota; Actinomycetes;
            Propionibacteriales; Nocardioidaceae; Micropruina.
REFERENCE   1  (bases 1 to 4018)
  AUTHORS   Abdulkadir,N., Saraiva,J.P., Zhang,J., Stolte,S., Gillor,O.,
            Harms,H. and Rocha,U.
  TITLE     Genome-centric analyses of 165 metagenomes show that mobile genetic
            elements are crucial for the transmission of antimicrobial
            resistance genes to pathogens in activated sludge and wastewater
  JOURNAL   Microbiol Spectr 12 (3), e0291823 (2024)
   PUBMED   38289113
REFERENCE   2  (bases 1 to 4018)
  AUTHORS   Abdulkadir,N.
  TITLE     Direct Submission
  JOURNAL   Submitted (24-MAR-2023) Environmental Microbiology, Helmholtz
            Center for Environmental Research -UFZ, Permoserstr 15, Leipzig
            04318, Germany
COMMENT     The annotation was added by the NCBI Prokaryotic Genome Annotation
            Pipeline (PGAP). Information about PGAP can be found here:
            https://www.ncbi.nlm.nih.gov/genome/annotation_prok/
            
            ##Genome-Assembly-Data-START##
            Assembly Method        :: SPAdes v. 3.11.1
            Genome Representation  :: Full
            Expected Final Version :: Yes
            Genome Coverage        :: Not Applicable
            Sequencing Technology  :: Illumina MiSeq
            ##Genome-Assembly-Data-END##
            
            ##Genome-Annotation-Data-START##
            Annotation Provider               :: NCBI
            Annotation Date                   :: 05/04/2023 04:57:10
            Annotation Pipeline               :: NCBI Prokaryotic Genome
                                                 Annotation Pipeline (PGAP)
            Annotation Method                 :: Best-placed reference protein
                                                 set; GeneMarkS-2+
            Annotation Software revision      :: 6.5
            Features Annotated                :: Gene; CDS; rRNA; tRNA; ncRNA
            Genes (total)                     :: 2,776
            CDSs (total)                      :: 2,750
            Genes (coding)                    :: 2,714
            CDSs (with protein)               :: 2,714
            Genes (RNA)                       :: 26
            tRNAs                             :: 24
            ncRNAs                            :: 2
            Pseudo Genes (total)              :: 36
            CDSs (without protein)            :: 36
            Pseudo Genes (ambiguous residues) :: 0 of 36
            Pseudo Genes (frameshifted)       :: 2 of 36
            Pseudo Genes (incomplete)         :: 32 of 36
            Pseudo Genes (internal stop)      :: 2 of 36
            ##Genome-Annotation-Data-END##
FEATURES             Location/Qualifiers
     source          1..4018
                     /organism="Micropruina sp."
                     /mol_type="genomic DNA"
                     /submitter_seqid="NODE_9367_length_4018_cov_4.131971"
                     /isolate="AMR_MDS_2195"
                     /isolation_source="Activated sludge of municipal
                     wastewater treatment plants"
                     /db_xref="taxon:2737536"
                     /environmental_sample
                     /geo_loc_name="Canada"
                     /lat_lon="49.25 N 123.1 W"
                     /collection_date="2015-06-16"
                     /metagenome_source="wastewater metagenome"
                     /note="metagenomic"
     gene            122..982
                     /locus_tag="PLF56_09225"
     CDS             122..982
                     /locus_tag="PLF56_09225"
                     /EC_number="1.6.5.2"
                     /inference="COORDINATES: protein motif:HMM:NF017206.2"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_function: GO:0016655 - oxidoreductase activity, acting
                     on NAD(P)H, quinone or similar compound as acceptor
                     [Evidence IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="SDR family oxidoreductase"
                     /protein_id="HOQ53780.1"
                     /translation="MSAVFVTGATGTVGSPVVDQLLQRGEKVVAAVRDKSHAASIPPG
                     AQPRLFDFAAAPAELRGSLDGCDRLFLMRPPPIADVQRYLFPVIDVARQAGVRQVVFL
                     SLQGVQANRGTPHHAVEAYLKRTGAPYTFLRPNFFMQNLSTTYAQRICDTGEIFVPAG
                     RARTAFIDARDIGRVAAAVLTSPRHLRKAYTLSGEQSLGYAAVARIMTEVLGRPIRYL
                     RPSEQQYLAALAADGWPPDYLAVQKMIYRVVRLNVSAMPNRTVRRLTGAPATRFDQFV
                     RDHAGVWSPA"
     gene            complement(987..1481)
                     /locus_tag="PLF56_09230"
     CDS             complement(987..1481)
                     /locus_tag="PLF56_09230"
                     /inference="COORDINATES: ab initio
                     prediction:GeneMarkS-2+"
                     /note="Derived by automated computational analysis using
                     gene prediction method: GeneMarkS-2+."
                     /codon_start=1
                     /transl_table=11
                     /product="hypothetical protein"
                     /protein_id="HOQ53781.1"
                     /translation="MEQYLWLLKSKELDLIRELEPERLAELDEDGLLALHKRVRRARK
                     KHTTNYRRGAAGKVAEAGGRGAARPASDKARGRAYVFEEALAIVSDELGRRAHQEAEA
                     LKDERLAKAQAGRSTGPDSQGATGATASKARTRTHSKTTGGVKRDASSRAQGKARQAK
                     KDAR"
     gene            1546..3006
                     /gene="cls"
                     /locus_tag="PLF56_09235"
     CDS             1546..3006
                     /gene="cls"
                     /locus_tag="PLF56_09235"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_017934305.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_function: GO:0008808 - cardiolipin synthase activity
                     [Evidence IEA];
                     GO_process: GO:0032049 - cardiolipin biosynthetic process
                     [Evidence IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="cardiolipin synthase"
                     /protein_id="HOQ53782.1"
                     /translation="MSDFFTDWKWWLLFAVDLVIRVAAIIIVPRNRRPTAATAWLLAI
                     YFIPLVGVALFLLIGNPRLPPARLRKQEQINDFIRQATAEIEFGSPRTDAPTWFDSLV
                     TLNRNLGAMPLSGDNTARLYPDYEGSIEAMAQAVREATAHVHVEFYILQVDAATEVFF
                     RALDEAVARGVTVRVLLDHWANRGKPHYKATTARLDAMGAQWHLMLPMRPFRGEYLRP
                     DLRNHRKLLVVDGRVAFTGSQNLTDSTYNLPANIRRGLHWVDLMVRLEGPIVASVNAV
                     FLGDWYSETGEVLTDEIDLFEVAAGPGHLDCQVVPSGPGFEFQNNLKLFMGLLYAARE
                     RAIIVSPYFVPDEALLVAIATSAQRGVQVELFVSEEGDQAMVYHAQRSYYEALLRAGV
                     TIWLYRKPFILHSKSMSIDDEVAVIGSSNMDMRSFGLNLEISVLVRGAGFVQDLREVE
                     AQYRAASRELTLDEWLQQPLRSTVLDNVARLTSALQ"
     gene            complement(3033..>4018)
                     /locus_tag="PLF56_09240"
     CDS             complement(3033..>4018)
                     /locus_tag="PLF56_09240"
                     /inference="COORDINATES: protein motif:HMM:NF012296.2"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_function: GO:0005506 - iron ion binding [Evidence IEA];
                     GO_function: GO:0016705 - oxidoreductase activity, acting
                     on paired donors, with incorporation or reduction of
                     molecular oxygen [Evidence IEA];
                     GO_function: GO:0020037 - heme binding [Evidence IEA]"
                     /codon_start=3
                     /transl_table=11
                     /product="cytochrome P450"
                     /protein_id="HOQ53783.1"
                     /translation="SHVSRFLQVPNGLDGEPHREARLLLDPFFSAERMAALAPDLERI
                     ADALVAAVPEGRPVDAVTELGSVYAVRAQSVWLGWPADIEPELLAWMDDNHAASRSGE
                     LGRTAEVAHRFDGIIRRLLMPRRAQPAGPAADVTAELMRLRHLDGTPWSEEQIVSVLR
                     NWTGGDLGSLALCAGVTLHWLATHPEAADALREADDDPFDRAVDEILRIDDPFVSNRR
                     AATRPTHLGGQPIGAGDKLVVNWTAANRDPAVFGDPDAYDPDGNAAANLVYGAGPHIC
                     PGRPLATLELRVLLRAVLAGHRVRLDPDSEPEREQPPVGGFRHVRVLLEPR"
BASE COUNT          565 a         1399 c         1398 g          656 t
ORIGIN      
        1 cccaggctga gcagcaggac caatgcgacc atccgccccc gcaacaccgc gaacccgagc
       61 gcgtgggtgc gccatggctc atccttgacg tggtggcagt ggccaagaaa gcggggcaga
      121 gatgagcgcg gtgttcgtga ccggagccac cggcacggtc gggtcgccgg tggtcgacca
      181 gttgctgcaa cgcggcgaga aggtggtggc cgccgtccgt gacaagtccc atgcggcatc
      241 cataccgccg ggcgcgcaac cgcgactgtt cgacttcgct gcggcgcctg cggagttgcg
      301 cgggtccctc gacggctgtg accggctgtt cctgatgcgg ccgccgccga tcgccgacgt
      361 gcagcgctac ctgtttccgg tcatcgacgt ggcgcggcag gcgggcgtcc ggcaggtggt
      421 gttcctgtcg ttgcagggcg tgcaggccaa tcggggcaca ccgcaccacg cggtcgaggc
      481 gtacctgaaa cggaccggcg ccccgtacac gtttttgcgc ccgaacttct tcatgcagaa
      541 cctgtccacc acctacgccc aacgcatctg cgacaccggt gagatcttcg tgccggccgg
      601 acgggcgcga accgccttca tcgacgcccg cgacatcgga cgggtcgccg ccgccgtgct
      661 cacctcgccc cggcacctgc gcaaggcgta cacgctgtcg ggtgagcaga gcctcggcta
      721 tgccgccgtc gcccggatca tgaccgaggt gctgggcagg ccgatccgct acctgcggcc
      781 ctcggaacag cagtacctgg ccgccctggc cgccgacggc tggccgccgg actacctggc
      841 cgtgcagaag atgatctacc gggtggtgcg actgaacgtg tcggcgatgc ccaaccgcac
      901 cgtgcgccgg ctcaccggtg caccggccac ccggttcgac cagttcgtgc gcgaccacgc
      961 cggggtctgg tcgcccgcct gacccgctag cgggcgtcct tcttggcctg ccgggccttg
     1021 ccctgcgcgc ggctggacgc gtcgcgcttg acaccgccgg tggtcttgct gtgggtgcgg
     1081 gtgcgggcct tggacgctgt cgcgcccgtc gcgccctggc tgtccgggcc ggtcgagcgg
     1141 ccggcctgcg ccttggcgag tcgttcgtcc ttgagggcct cggcctcttg gtgcgcgcgg
     1201 cgtccgagct cgtcgctgac gatggccagc gcctcttcga acacgtacgc acggccccgg
     1261 gctttgtcgg aggccggcct ggccgcaccg cgtccgccgg cctcggccac cttgcccgca
     1321 gccccgcgcc ggtagttggt ggtgtgcttc ttgcgcgccc ggcgcacccg cttgtgcagg
     1381 gcgagcaggc cgtcctcgtc gagctcggcc agccgctcgg gctcgagttc gcggatcagg
     1441 tcgagttctt tgctcttgag taaccacaag tactgttcca tggcgccata ctgccagccg
     1501 gatcgctaca ttgggcgcga tcatcgtcca gctgtggggt gcgtggtgtc tgacttcttc
     1561 accgactgga agtggtggct gctgttcgcc gtcgacctgg tcattcgcgt cgccgccatc
     1621 atcatcgttc cgcgcaatcg ccggcccacg gcggccaccg cctggttgct ggcgatctac
     1681 ttcattccgc tggtcggggt ggcgctgttc ctgctgatcg gcaacccgcg actgccgccg
     1741 gcccggttgc gtaagcagga gcagatcaac gacttcatcc ggcaggcgac cgccgagatc
     1801 gagttcgggt cgccgcgcac cgatgcgccc acgtggttcg actcgttggt caccctcaac
     1861 cgcaatctcg gggccatgcc gctcagtggc gacaacaccg cccggctgta ccccgactac
     1921 gagggcagca tcgaggcaat ggcacaggcc gtccgcgagg ccaccgcaca cgtgcacgtc
     1981 gagttctaca tactgcaggt cgacgccgcc accgaggtgt tcttccgggc gctggacgag
     2041 gctgtcgcgc gcggggtcac cgtgcgggtg ctgctcgacc attgggccaa ccgcggcaag
     2101 ccccactaca aagcgaccac cgcccggctg gacgcgatgg gtgcccagtg gcatctgatg
     2161 ctgccgatgc gcccgttccg cggcgagtac ctgcgacccg accttcgcaa ccaccgcaag
     2221 ctgctggtgg tcgatggacg ggtggcgttc accgggtcgc agaacctgac cgactcgacc
     2281 tacaacctgc ccgcgaacat tcgccgcggg ctgcactggg tcgacctgat ggtgcgactc
     2341 gagggcccga tcgtggccag cgtcaacgcg gtctttctgg gcgactggta cagcgagacc
     2401 ggcgaggtgc tgaccgatga gatcgacctg ttcgaggtcg cggccggccc gggacacctg
     2461 gactgccaag tcgtaccgtc cgggcccggg ttcgagtttc agaacaacct gaagctgttc
     2521 atgggcctgc tgtacgcggc gcgcgagcgg gccatcatcg tgtcgcccta cttcgtgccg
     2581 gatgaggccc tgctggtggc catcgccacg tcagcgcagc gcggggtgca ggtcgaactg
     2641 ttcgtctccg aagagggcga ccaggccatg gtctaccacg cccagcgcag ctactacgag
     2701 gcgctgctgc gggccggggt gacgatctgg ctgtaccgga agccgttcat tctgcattcc
     2761 aagtcgatgt cgatcgatga cgaggtggcg gtgatcggat ccagcaacat ggacatgcgt
     2821 tcgttcggcc tcaacctcga aatctcggtg ctggtgcgcg gcgccggctt cgtgcaggat
     2881 ctgcgcgagg tcgaggccca gtaccgggcg gcctcgcgag agctgacgct ggacgagtgg
     2941 ttgcagcagc cgctgcgctc gaccgtgctc gacaacgtgg cccggctcac ctcggcgctg
     3001 cagtaggggg caggacgccg aacagcgatg gatcagcgcg gctcgagcag cacccgcacg
     3061 tgccggaacc cgcccaccgg cggctgctca cgttcgggct cgctgtcggg atcgagccgc
     3121 acacggtgcc cggccaacac ggctcgcagc agcacccgca gttcgagggt ggcgagcgga
     3181 cgtccggggc agatgtgcgg cccggcgccg tagaccaggt tggctgcggc gttgccgtcg
     3241 ggatcgtagg cgtcggggtc gccgaacacc gccgggtcgc ggttggccgc ggtccagttg
     3301 accaccagct tgtcgccggc cccgatcggt tgtccgccca ggtgggtggg acgggtggcg
     3361 gcccgccggt tggagacgaa cgggtcgtcg atgcgcagga tttcgtccac ggcccggtcg
     3421 aacgggtcgt cgtcggcctc gcgcagggcg tccgcagcct cgggatgggt ggccagccag
     3481 tgcagggtga ccccggcgca cagggccagg gaacccaggt cgccgccggt ccagttgcgc
     3541 agcaccgaca cgatctgctc ctccgaccag ggcgtgccgt ccaggtgccg caatcgcatc
     3601 agttcggcgg tcacatcggc ggcgggcccg gccggctgcg cgcggcgcgg catcaacagc
     3661 cgccggatga tgccgtcgaa acggtgtgcc acctcggcgg ttctgcccag ttcgcccgac
     3721 cggctggcgg cgtggttgtc gtccatccag gccagcagtt ccggctcgat gtcggccggc
     3781 cagccgagcc agaccgattg cgcccgcacg gcatagaccg atcccagctc ggtgaccgcg
     3841 tccaccggcc ggccctcggg cacggcggcc accagtgcgt ccgcgatccg ctccaggtcg
     3901 ggggccagcg ctgccatgcg ttcggccgag aagaacgggt cgagcagcag ccgggcctcg
     3961 cggtgcggct cgccgtccag gccgttgggc acctgcagga accgcgacac gtggctcg
//
LOCUS       DAOSUG010000270         4011 bp    DNA     linear   ENV 10-JUN-2024
DEFINITION  MAG TPA_asm: Micropruina sp. isolate AMR_MDS_2195
            NODE_9386_length_4011_cov_4.141052, whole genome shotgun sequence.
ACCESSION   DAOSUG010000270 DAOSUG010000000
VERSION     DAOSUG010000270.1
DBLINK      BioProject: PRJNA925477
            BioSample: SAMN32864262
            Sequence Read Archive: SRR4236673
KEYWORDS    WGS; ENV; Metagenome Assembled Genome; MAG; Third Party Data; TPA;
            TPA:assembly.
SOURCE      Micropruina sp. (wastewater metagenome)
  ORGANISM  Micropruina sp.
            Bacteria; Bacillati; Actinomycetota; Actinomycetes;
            Propionibacteriales; Nocardioidaceae; Micropruina.
REFERENCE   1  (bases 1 to 4011)
  AUTHORS   Abdulkadir,N., Saraiva,J.P., Zhang,J., Stolte,S., Gillor,O.,
            Harms,H. and Rocha,U.
  TITLE     Genome-centric analyses of 165 metagenomes show that mobile genetic
            elements are crucial for the transmission of antimicrobial
            resistance genes to pathogens in activated sludge and wastewater
  JOURNAL   Microbiol Spectr 12 (3), e0291823 (2024)
   PUBMED   38289113
REFERENCE   2  (bases 1 to 4011)
  AUTHORS   Abdulkadir,N.
  TITLE     Direct Submission
  JOURNAL   Submitted (24-MAR-2023) Environmental Microbiology, Helmholtz
            Center for Environmental Research -UFZ, Permoserstr 15, Leipzig
            04318, Germany
COMMENT     The annotation was added by the NCBI Prokaryotic Genome Annotation
            Pipeline (PGAP). Information about PGAP can be found here:
            https://www.ncbi.nlm.nih.gov/genome/annotation_prok/
            
            ##Genome-Assembly-Data-START##
            Assembly Method        :: SPAdes v. 3.11.1
            Genome Representation  :: Full
            Expected Final Version :: Yes
            Genome Coverage        :: Not Applicable
            Sequencing Technology  :: Illumina MiSeq
            ##Genome-Assembly-Data-END##
            
            ##Genome-Annotation-Data-START##
            Annotation Provider               :: NCBI
            Annotation Date                   :: 05/04/2023 04:57:10
            Annotation Pipeline               :: NCBI Prokaryotic Genome
                                                 Annotation Pipeline (PGAP)
            Annotation Method                 :: Best-placed reference protein
                                                 set; GeneMarkS-2+
            Annotation Software revision      :: 6.5
            Features Annotated                :: Gene; CDS; rRNA; tRNA; ncRNA
            Genes (total)                     :: 2,776
            CDSs (total)                      :: 2,750
            Genes (coding)                    :: 2,714
            CDSs (with protein)               :: 2,714
            Genes (RNA)                       :: 26
            tRNAs                             :: 24
            ncRNAs                            :: 2
            Pseudo Genes (total)              :: 36
            CDSs (without protein)            :: 36
            Pseudo Genes (ambiguous residues) :: 0 of 36
            Pseudo Genes (frameshifted)       :: 2 of 36
            Pseudo Genes (incomplete)         :: 32 of 36
            Pseudo Genes (internal stop)      :: 2 of 36
            ##Genome-Annotation-Data-END##
FEATURES             Location/Qualifiers
     source          1..4011
                     /organism="Micropruina sp."
                     /mol_type="genomic DNA"
                     /submitter_seqid="NODE_9386_length_4011_cov_4.141052"
                     /isolate="AMR_MDS_2195"
                     /isolation_source="Activated sludge of municipal
                     wastewater treatment plants"
                     /db_xref="taxon:2737536"
                     /environmental_sample
                     /geo_loc_name="Canada"
                     /lat_lon="49.25 N 123.1 W"
                     /collection_date="2015-06-16"
                     /metagenome_source="wastewater metagenome"
                     /note="metagenomic"
     gene            complement(<1..474)
                     /locus_tag="PLF56_09245"
     CDS             complement(<1..474)
                     /locus_tag="PLF56_09245"
                     /inference="COORDINATES: ab initio
                     prediction:GeneMarkS-2+"
                     /note="Derived by automated computational analysis using
                     gene prediction method: GeneMarkS-2+."
                     /codon_start=1
                     /transl_table=11
                     /product="hypothetical protein"
                     /protein_id="HOQ53784.1"
                     /translation="MSQNQFPGQNPQPGGQPYPQGGQPQLQPGGQPYPQGGQPNLQPG
                     GQPYPQGGQPYPQPAGQPFQQGGQPYPQGGQPHPQSQYPPAGGQYGQVPLGAQAGPQA
                     GFQPPPPKPKGNGMIIGVVVAALVAVGLAGWLLMILFGGGNEPAPTPSPTVTSTSA"
     gene            complement(471..1637)
                     /locus_tag="PLF56_09250"
     CDS             complement(471..1637)
                     /locus_tag="PLF56_09250"
                     /inference="COORDINATES: protein motif:HMM:NF024760.2"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="zinc ribbon domain-containing protein"
                     /protein_id="HOQ53785.1"
                     /translation="MQCTRCHADVPEAAHYCQVCGLDTHNDDRARRTSFAAKPNEPVV
                     SLRLVSTIMPRGSAVRPMTYQFALGIALLATVLAAATGALPIAVLIAAFAMPIVYIVY
                     LYDVNLWEDEPVLVTGLAFVLTAALGGFWTWLWLSWRGPLTPGSGFDEAAPLPSITGL
                     LLAAVLAPLVGEVIRQVGPVILASRPEFDDLMDGLTFGVISGVGFATADTLVKHWALI
                     TGGFAGSADAGTWISLLVLEGFVKPLVMGTATGIACAEFSGLGEGCDGFTPRYYRAFA
                     EAVGANVLYAGGVYLLGFLGNQVLALVLSILWGLAILSVLVLRIRTALHRGLMEAALE
                     STARARGVGPGLEFCPSCEMPLMPGAEFCSNCGISVRAAAKVPHSKASGEEGRA"
     gene            complement(1763..2503)
                     /locus_tag="PLF56_09255"
     CDS             complement(1763..2503)
                     /locus_tag="PLF56_09255"
                     /inference="COORDINATES: ab initio
                     prediction:GeneMarkS-2+"
                     /note="Derived by automated computational analysis using
                     gene prediction method: GeneMarkS-2+."
                     /codon_start=1
                     /transl_table=11
                     /product="hypothetical protein"
                     /protein_id="HOQ53786.1"
                     /translation="MTGTHPPNYENYPPPTQDQRPPVGESTREPEGWTVVPAPTAPPL
                     SPGRTSGPSRRGVLGLIGVLGVGLVGVVAYADLATGVGGLATGESSSWTDPAPEEDPE
                     AEEFDLDGLTVTAPRGWEAVSSTAHRLVLRNGANTVTFLHYEAGQDASAMDEARQQLR
                     RYAADVRSAKETSADTSTNEDGVEKAEVVLTGKVDDAAVDVVSSAAIDPADDYAAAAV
                     VSVLAHKGATGIAGEVKRMRQELFDQLG"
     gene            complement(2507..3172)
                     /gene="coaE"
                     /locus_tag="PLF56_09260"
     CDS             complement(2507..3172)
                     /gene="coaE"
                     /locus_tag="PLF56_09260"
                     /EC_number="2.7.1.24"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_013864945.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_function: GO:0004140 - dephospho-CoA kinase activity
                     [Evidence IEA];
                     GO_function: GO:0005524 - ATP binding [Evidence IEA];
                     GO_process: GO:0015937 - coenzyme A biosynthetic process
                     [Evidence IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="dephospho-CoA kinase"
                     /protein_id="HOQ53787.1"
                     /translation="MSKAHEQAPQVLTVGLTGGIASGKSAAADRLTELGAVIIDADLL
                     AREVVAPGTPGLAAIEERFGPSILTTGPDGLAHLDRAALGALVFSDEQARRDLEAIVH
                     PAVRARAAELTRAAPSDAVVVQVIPLLVETGQQGAFDRVVVVDIDPERQLSRLMSRNQ
                     LNPRQARARIAAQAGRAERLAAADHVLDNNGTPEQLHAQVDALWGRLRTASAGPAPSG
                     GTV"
     gene            complement(3236..>4011)
                     /locus_tag="PLF56_09265"
     CDS             complement(3236..>4011)
                     /locus_tag="PLF56_09265"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_016456583.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_function: GO:0003676 - nucleic acid binding [Evidence
                     IEA]"
                     /codon_start=3
                     /transl_table=11
                     /product="S1 RNA-binding domain-containing protein"
                     /protein_id="HOQ53788.1"
                     /translation="GVDGLVHVSELSWKHIDHPSEVVAVGDEVTVEVLDVDMDRERVS
                     LSLKATQEDPWQTFARTHQIGQIVPGKVTKLVPFGAFVRVEEGIEGLVHVSELAERHV
                     EIPEQVVSVNDEVMVKIIDIDLERRRISLSLKQANEGVDMHADDFDPALYGMSASYDD
                     QGNYIYPEGFDPETQEWKPGYEEQQAAWERQYAEAQARWEAHKQQVKDAEVAGAEAAV
                     AEVSTAAYSAGGVDDAAPEGSLASDEALQALRDRLTGNR"
BASE COUNT          604 a         1439 c         1364 g          604 t
ORIGIN      
        1 cgccgaggtg gaggtcaccg tcggggacgg ggtgggcgcg ggttcgttgc cgccgccgaa
       61 caggatcatc aatagccagc cggccaggcc gaccgcgacg agggccgcga ccaccacgcc
      121 gatgatcatg ccgttgccct tgggcttggg cggcggtggc tgaaagcccg cctgcgggcc
      181 cgcctgggcg cctagcggca cctgtccata ctggccaccg gccggcgggt actgggactg
      241 cgggtggggt tgcccgccct gcgggtacgg ctgaccgccc tgttggaagg gctggccggc
      301 cggctgcggg tagggctggc cgccctgcgg gtacggctga ccgcccggct gcaggttcgg
      361 ctggccgccc tgcggatacg gctgaccgcc cggctgcagc tgcggctggc cgccctgcgg
      421 atacggctga ccgcccggct gcgggttctg acccgggaac tggttctggc tcatgcccgt
      481 ccctcctcac cggacgcctt ggagtgcggc accttggccg ccgcacgcac gctgatcccg
      541 cagttggagc agaactccgc accgggcatc agcggcatct cgcaggaggg acagaactcg
      601 aggccggggc cgaccccccg ggctcgtgcc gtggactcca acgccgcctc catcaggccg
      661 cggtgcaggg cggtgcgaat gcgaagcacc agcaccgaca ggatcgccag cccccacaga
      721 atggacagca ccagcgcgag cacctggttg ccgagaaagc cgagcaggta gaccccgccg
      781 gcatacaaca cgttcgcgcc gaccgcctcg gcgaatgcgc ggtagtaccg cggggtgaag
      841 ccgtcgcagc cctcccccag acccgagaac tctgcgcacg cgatgccggt ggcggtaccc
      901 atcaccagcg gcttgacgaa accctcgagc accagcagcg agatccaggt gccggcgtcc
      961 gccgagccgg cgaatccccc ggtgatcagc gcccagtgct tcaccagcgt gtcggccgtg
     1021 gcgaacccga cccccgagat gacgccgaag gtcagcccgt ccatcaggtc gtcgaactcg
     1081 ggccgcgagg cgagaatgac cgggcccacc tgccggatca cctcgccgac caacggtgcc
     1141 agcacggccg ccagcaacag gcccgtgatg gacggcagcg gcgccgcctc gtcgaaaccc
     1201 gaaccgggcg tcagtgggcc gcgccaactc agccacagcc acgtccagaa acctcctaga
     1261 gcagccgtca gcacgaaggc caggccggtg accagtactg gttcgtcttc ccacaggttc
     1321 acgtcgtaga ggtagacgat gtagacgatc ggcatggcga aggcagcgat cagcaccgct
     1381 atcggcagcg cgccggtcgc ggcagcgagc accgttgcca gcagtgcgat gccgagcgcg
     1441 aactgatagg tcatcggacg cacggccgaa ccgcgcggca tgatcgtcga gaccagccgc
     1501 agcgacacca cgggctcgtt cggcttcgcc gcgaacgacg ttctgcgggc gcggtcgtcg
     1561 ttgtgcgtat caagcccgca cacctgacag tagtgcgccg cctcgggtac gtccgcgtgg
     1621 caacgggtgc actgcacagt ggtttccttc cggtcggttc ggcgacccct ggggccgggt
     1681 tgggccagtc tcgcaagcca ggggatcact cgagccgttc tgatacgtcg acccggcggt
     1741 tgcgacctcg gctgccggcc ggtcaaccca actggtcgaa caactcctgg cgcatccgct
     1801 tcacctcgcc ggcgatcccc gtggcgcctt tgtgggcgag caccgagacc accgcggcgg
     1861 cggcgtagtc atcggccggg tcgatcgccg cggatgagac gacgtccacg gccgcgtcgt
     1921 cgaccttgcc ggtcagcacc acctcggcct tctccacccc gtcctcattc gtggacgtgt
     1981 cggcgctggt ttccttggcc gagcggacgt ccgccgcgta acgacggagc tgctgacggg
     2041 cctcgtccat ggcactggcg tcctgcccgg cttcgtagtg cagaaaggtg acggtgttgg
     2101 cgccgttgcg caacaccagc cgatgcgccg tggacgacac cgcctcccaa ccacgcggtg
     2161 cagtcacggt gagaccgtcc agatcgaact cctcggcctc agggtcttcc tcaggcgcag
     2221 gatcggtcca ggacgaggat tcgccggtgg ccaggccgcc cacgccggtg gcgagatcgg
     2281 cgtacgcgac cacacccacc aggccgaccc ccagcacgcc gatcaaaccc agcacgccgc
     2341 ggcggctcgg gcctgacgta cgaccggggc ttaacggtgg ggccgtcggc gccggcacga
     2401 ccgtccaacc ctccggctcc cttgtcgatt caccgacggg cggccgctgg tcctgcgtcg
     2461 gcggcggata gttctcgtag ttcggagggt gggtgccggt catgggtcac actgtgccac
     2521 cggagggtgc cggcccggcg gaggccgtgc gcagtcgccc ccacagggcg tccacctgtg
     2581 cgtgcagttg ctcgggcgtg ccgttgttgt cgagcacgtg atcggccgcg gcgagccgct
     2641 ccgcacggcc ggcctgcgcc gcgatccggg cccgggcctg cctggggttc agctgattgc
     2701 gactcatcaa ccggctgagc tgccgctcgg gatcgatgtc gaccaccacc acgcgatcga
     2761 aggcaccctg ctgcccggtc tcgaccagca gcggaatcac ctgcaccacc accgcatcgg
     2821 acggcgccgc ccgagtcagt tcggccgccc tggcccggac ggcggggtgc acgatcgcct
     2881 cgaggtcacg ccgggcctgc tcgtcggaga agaccagcgc gcccagggcg gcacgatcga
     2941 ggtgggcgag tccgtccgga ccggtggtga gaattgatgg cccgaatcgc tcctcgatgg
     3001 cggccagccc cggggttccc ggcgcgacca cctcgcgcgc cagcaggtcg gcgtcgatga
     3061 tcacagcgcc cagttcggtc agccgatcgg ccgcagcgct cttgccggac gcgatgcccc
     3121 cggtgagtcc gacggtcaac acctgcggtg cctgctcatg cgccttgctc atgggtctga
     3181 ttgtgacgcg aaacggcccc caccgcgtgg tgggggccgt ttcgtgttca gcggttcagc
     3241 ggttgccggt gagccggtcg cgcaacgcct gcagcgcctc gtccgaggcg agcgagccct
     3301 cgggggccgc gtcgtcgacg ccgcccgcgg agtacgcggc ggtgctgacc tcggccacgg
     3361 cggcctcagc cccagcgacc tcggcgtcct tgacctgctg cttgtgcgcc tcccagcggg
     3421 cctgggcctc ggcgtactgc cgctcccacg ccgcctgctg ctcttcgtag ccgggcttcc
     3481 actcctgggt ctcgggatcg aagccctcgg ggtagatgta gttgccctgg tcgtcgtagg
     3541 acgcgctcat gccgtacagc gccgggtcga agtcgtcggc gtgcatgtcg acaccctcgt
     3601 tggcctgctt gagcgacagc gagatgcggc gacgctcgag atcgatgtcg atgatcttga
     3661 ccatgacctc gtcgttgacg ctgaccacct gctcggggat ctcgacgtga cgctcggcca
     3721 gctcggacac gtgcaccagg ccctcgatgc cctcttccac ccggacgaag gcgccgaagg
     3781 gcaccagctt ggtgaccttg cccggcacga tctgaccgat ctggtgggta cgggcgaagg
     3841 tctgccacgg atcttcctgc gtggccttca gcgacagcga gacgcgctcg cgatccatgt
     3901 cgacatcgag cacctcgacg gtgacctcgt cgccgaccgc gaccacctcg gacggatggt
     3961 cgatgtgctt ccacgacagc tcggagacgt gcaccaggcc gtccacgccg c
//
LOCUS       DAOSUG010000271         3997 bp    DNA     linear   ENV 10-JUN-2024
DEFINITION  MAG TPA_asm: Micropruina sp. isolate AMR_MDS_2195
            NODE_9419_length_3997_cov_3.850837, whole genome shotgun sequence.
ACCESSION   DAOSUG010000271 DAOSUG010000000
VERSION     DAOSUG010000271.1
DBLINK      BioProject: PRJNA925477
            BioSample: SAMN32864262
            Sequence Read Archive: SRR4236673
KEYWORDS    WGS; ENV; Metagenome Assembled Genome; MAG; Third Party Data; TPA;
            TPA:assembly.
SOURCE      Micropruina sp. (wastewater metagenome)
  ORGANISM  Micropruina sp.
            Bacteria; Bacillati; Actinomycetota; Actinomycetes;
            Propionibacteriales; Nocardioidaceae; Micropruina.
REFERENCE   1  (bases 1 to 3997)
  AUTHORS   Abdulkadir,N., Saraiva,J.P., Zhang,J., Stolte,S., Gillor,O.,
            Harms,H. and Rocha,U.
  TITLE     Genome-centric analyses of 165 metagenomes show that mobile genetic
            elements are crucial for the transmission of antimicrobial
            resistance genes to pathogens in activated sludge and wastewater
  JOURNAL   Microbiol Spectr 12 (3), e0291823 (2024)
   PUBMED   38289113
REFERENCE   2  (bases 1 to 3997)
  AUTHORS   Abdulkadir,N.
  TITLE     Direct Submission
  JOURNAL   Submitted (24-MAR-2023) Environmental Microbiology, Helmholtz
            Center for Environmental Research -UFZ, Permoserstr 15, Leipzig
            04318, Germany
COMMENT     The annotation was added by the NCBI Prokaryotic Genome Annotation
            Pipeline (PGAP). Information about PGAP can be found here:
            https://www.ncbi.nlm.nih.gov/genome/annotation_prok/
            
            ##Genome-Assembly-Data-START##
            Assembly Method        :: SPAdes v. 3.11.1
            Genome Representation  :: Full
            Expected Final Version :: Yes
            Genome Coverage        :: Not Applicable
            Sequencing Technology  :: Illumina MiSeq
            ##Genome-Assembly-Data-END##
            
            ##Genome-Annotation-Data-START##
            Annotation Provider               :: NCBI
            Annotation Date                   :: 05/04/2023 04:57:10
            Annotation Pipeline               :: NCBI Prokaryotic Genome
                                                 Annotation Pipeline (PGAP)
            Annotation Method                 :: Best-placed reference protein
                                                 set; GeneMarkS-2+
            Annotation Software revision      :: 6.5
            Features Annotated                :: Gene; CDS; rRNA; tRNA; ncRNA
            Genes (total)                     :: 2,776
            CDSs (total)                      :: 2,750
            Genes (coding)                    :: 2,714
            CDSs (with protein)               :: 2,714
            Genes (RNA)                       :: 26
            tRNAs                             :: 24
            ncRNAs                            :: 2
            Pseudo Genes (total)              :: 36
            CDSs (without protein)            :: 36
            Pseudo Genes (ambiguous residues) :: 0 of 36
            Pseudo Genes (frameshifted)       :: 2 of 36
            Pseudo Genes (incomplete)         :: 32 of 36
            Pseudo Genes (internal stop)      :: 2 of 36
            ##Genome-Annotation-Data-END##
FEATURES             Location/Qualifiers
     source          1..3997
                     /organism="Micropruina sp."
                     /mol_type="genomic DNA"
                     /submitter_seqid="NODE_9419_length_3997_cov_3.850837"
                     /isolate="AMR_MDS_2195"
                     /isolation_source="Activated sludge of municipal
                     wastewater treatment plants"
                     /db_xref="taxon:2737536"
                     /environmental_sample
                     /geo_loc_name="Canada"
                     /lat_lon="49.25 N 123.1 W"
                     /collection_date="2015-06-16"
                     /metagenome_source="wastewater metagenome"
                     /note="metagenomic"
     gene            131..823
                     /locus_tag="PLF56_09270"
     CDS             131..823
                     /locus_tag="PLF56_09270"
                     /inference="COORDINATES: protein motif:HMM:NF014881.2"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="hypothetical protein"
                     /protein_id="HOQ53789.1"
                     /translation="MPSRGRPGPDPATPLTGRSPASEIVVPDLESSVRWHQHDFPHPL
                     ARWHTHPEVEVHLIRRSTGLAFVGDYIGPFGPGHFVLVGSQLPHNWLSDLEPDEMVAG
                     RDVVILGRGFTLGRPLSMLLSLKRPGGNAAVTVVHTGVPNWADYTRRADIVIAAAGVP
                     GILRPEHITAGRVVVGGGVRYEGRRLLPDVDEACNEVAGWITPRVGGVGPTTIAMLFR
                     NCVEAAEGRAAR"
     gene            978..1346
                     /locus_tag="PLF56_09275"
     CDS             978..1346
                     /locus_tag="PLF56_09275"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_013863476.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_function: GO:0003677 - DNA binding [Evidence IEA];
                     GO_function: GO:0003700 - DNA-binding transcription factor
                     activity [Evidence IEA];
                     GO_process: GO:0006355 - regulation of DNA-templated
                     transcription [Evidence IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="metalloregulator ArsR/SmtB family transcription
                     factor"
                     /protein_id="HOQ53790.1"
                     /translation="MSLTRSQRPLYEVKAGLFKGLAHPFRIRILELLSAAAEVSVTDL
                     QNETGLEASHLSQHLAVLRRHRLVDSERRASHVYYRLADAKVADLLTVARELLLSVLA
                     TDREMLAEAIALPAIASANR"
     gene            1343..3028
                     /locus_tag="PLF56_09280"
     CDS             1343..3028
                     /locus_tag="PLF56_09280"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_013863475.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_component: GO:0016020 - membrane [Evidence IEA];
                     GO_function: GO:0015103 - inorganic anion transmembrane
                     transporter activity [Evidence IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="SulP family inorganic anion transporter"
                     /protein_id="HOQ53791.1"
                     /translation="MSLSGAWTSGVRYVRALLPGADDAATVAANWRGDLLAGLTVGIV
                     ALPLALGFGVSAGLSAEAGLVTAIVAGLIAAVFGGSHVQVSGPTGAMVVVLAPIVAGQ
                     GVAAVTAVTLLAGVIVLVAGLLKLGRAVSFIPWPVIEGFTLGIAVIIFAQQIPALTGA
                     HAGSAAHSSNAVVAAVQALAGADAGYLLWSLGAVLLVAACMLLAPRLHAALPGSLIAI
                     VVVAVLAVITGAPLAVIGALPHALPAPTWPVLDAQVIQHLGGAALTVAALAAIESLLS
                     ARVAASLADTGAFDPDRELVGQGLASIGSSLFGGMPATGAIARTAVNVRSGGRSRLAA
                     IVHALVLLGVVSFAAGPVGRIPLAALSGVLMITAIRMIDRDTVSSIMRSTRADAGGFV
                     LTAVVTVSFDLIMAVLIGVVVAGVFAIRNLSKASRVQREAIRGEPQIGDDQIAIIRFD
                     GPLIFAAADRVFDEVQNLDGVEVVILRMSQLELVDATGARILNDIVTTLERRGVTVLI
                     KGVRPGHHELFRSVGVLDALRHADHLFDTLPPAIEHARSHVRRERIERWARAH"
     gene            complement(3094..3582)
                     /locus_tag="PLF56_09285"
     CDS             complement(3094..3582)
                     /locus_tag="PLF56_09285"
                     /inference="COORDINATES: ab initio
                     prediction:GeneMarkS-2+"
                     /note="Derived by automated computational analysis using
                     gene prediction method: GeneMarkS-2+."
                     /codon_start=1
                     /transl_table=11
                     /product="hypothetical protein"
                     /protein_id="HOQ53792.1"
                     /translation="MHADRLLIPPLLVGERVTLRLTDPARDLIGFVTALEPLTIEDRH
                     GRLHPIAPGTVQAARRVGVSLGRDPVRTPRALLDELASRAGLAGDPELHRISDLLAGR
                     AAPAEVFTERGEWSRGADRARVEGEWLSTNVEDPELLAALCWWASRQNARSVQVRVRL
                     EG"
     gene            3654..3974
                     /locus_tag="PLF56_09290"
     CDS             3654..3974
                     /locus_tag="PLF56_09290"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_015069892.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="ferredoxin family protein"
                     /protein_id="HOQ53793.1"
                     /translation="MTYIITQPCVDVKDLACVEECPVDCIYEGNRMLYIQPDECVDCG
                     ACEPVCPVEAIYYEDDVPADQKVYYDVNVNFFDTVGSPGGAARQGPIDADHPYVAALP
                     PQGE"
BASE COUNT          562 a         1380 c         1398 g          657 t
ORIGIN      
        1 ccctggatct atagcgtcag aaccgcagaa ctggtactaa attgtcggca gagagtctgt
       61 cggagaccat ctggtcgatc agctgcccag tgatcggcct attgccgggc cgacgtgcga
      121 ggaggtacgg gtgccgtcac ggggacgacc gggtcccgat ccggccacgc cgttgaccgg
      181 tcgttccccg gccagcgaaa tcgtggtgcc cgatctggag agcagcgtcc gatggcacca
      241 gcacgatttc ccgcacccgt tggcgcggtg gcacacccat cccgaggtgg aagtgcacct
      301 gatccgtcgg agtaccggac tcgccttcgt gggcgattac atcggaccgt tcggtcccgg
      361 gcatttcgtg ctggtcgggt cacaattgcc gcacaattgg ttgtccgatc tcgagcccga
      421 cgaaatggtc gcgggtcgcg acgtggtgat tctgggccgc ggcttcacac tgggccggcc
      481 gctgtcgatg ctgctgtcgc tgaagcgacc gggcggcaac gcggcggtca ccgtggtgca
      541 caccggagtg ccgaactggg ccgactacac ccggcgcgcg gacatcgtga tcgcggcggc
      601 gggggtgccc ggcatcctgc ggcccgagca catcaccgct ggtcgggtcg tggtcggcgg
      661 cggcgtccgg tatgagggtc ggcggctgct gcccgacgtg gacgaggcct gcaacgaggt
      721 ggccggctgg atcacccccc gggtcggagg agtgggtccg accaccatcg ccatgctgtt
      781 ccgcaactgc gtcgaggcgg ccgaggggcg ggcggctcgc tgagcgaggt gggtgccgca
      841 acagatcacg gttcccagac cggtcgcgtc gtcggtcgcg catgatgaga tccggaccgg
      901 gccgtggaat ctcgaacgct ggaccaacgt gcacggcttg gcaggggact ttggcttgac
      961 gatatgatga gttcatcatg tcactcacac gctcgcagcg tccgctctac gaagtgaagg
     1021 cgggcctgtt caagggcctc gcgcacccct ttcgaatccg catcctcgaa ctgctcagtg
     1081 ccgccgctga agtgagcgtg accgacctgc agaacgagac cgggctggag gcctcgcatc
     1141 tgtcccagca cttggcggtg ttgcgccgcc accggctggt cgattccgag cgccgggcca
     1201 gtcacgtcta ctaccggttg gccgacgcga aggtggccga cctgctcacc gtcgcacgcg
     1261 aactgctgct cagcgtgctg gcgacggacc gcgagatgct ggccgaggcg atcgcgctgc
     1321 cggccatcgc aagcgcgaac cgatgagtct cagcggtgcc tggacgagcg gcgtccgcta
     1381 cgtccgtgcc ctgctacccg gagcggacga cgcggccacg gtcgcggcca actggcgcgg
     1441 cgatctgctg gccggcctca ccgtcggcat cgtggcactg ccgctcgcgc tcggcttcgg
     1501 agtcagcgcg gggctgagcg ccgaggccgg gctggtcacg gcaatcgtcg ccggcctgat
     1561 cgcggccgtg ttcgggggct cgcacgtgca ggtgtccgga cccaccggtg cgatggtggt
     1621 ggtgctggcc ccgatcgtcg ccggccaggg ggtcgcagcg gtcaccgcgg tcacgctgct
     1681 cgccggggtg atcgtgctcg tggccggact gctcaaactc ggccgggcgg tctcgttcat
     1741 tccgtggccg gtgatcgagg gcttcacgct gggcatcgcg gtgatcatct tcgcccaaca
     1801 gatcccggcg ttgaccggtg cgcacgcggg cagcgccgcg cacagctcga acgcggtggt
     1861 ggcggccgtc caggcgctgg ccggtgcgga cgccggctat ctgctctggt cgctcggcgc
     1921 cgtgctgctc gtcgccgcct gcatgctgct ggcgccccgg ctgcacgccg cactgcccgg
     1981 ctcgctgatc gcgatcgtgg tggtcgcggt actcgccgtg atcaccggcg cgccgctggc
     2041 cgtgatcggc gccctgcccc acgcgctgcc cgcgccgacg tggccggtgc tggacgcgca
     2101 ggtgatccag cacctcgggg gtgccgccct gaccgtggcg gcgctggccg cgatcgagtc
     2161 cttgttgtcg gcccgggtcg ccgcgtccct ggccgacacc ggggccttcg atcccgaccg
     2221 cgaactcgtc ggccagggcc tcgcgtcgat cggatcgtcg ctcttcggcg gcatgccggc
     2281 caccggcgcg atcgcccgga cggcggtcaa cgtccggtcc ggcggacgca gcaggctggc
     2341 ggccatcgtt catgcgctgg tgctgctggg cgtggtgtcc ttcgccgccg gaccggtcgg
     2401 acgcatcccg ctggctgccc tctcgggcgt gctgatgatc accgcgatcc gcatgatcga
     2461 ccgcgacacc gtctcgtcga tcatgcgctc cacccgggcg gacgccggcg gcttcgtgct
     2521 gacggccgtt gtcacggtct cgttcgacct gatcatggcg gtgctgatcg gggtggtcgt
     2581 cgccggcgtg tttgcgatcc gcaacctgtc gaaggccagt cgggtgcagc gcgaggcgat
     2641 ccgcggcgag ccgcagatcg gcgacgacca gatcgcgatc atccgcttcg acggcccgtt
     2701 gatcttcgcc gccgccgacc gggtcttcga cgaggtgcag aacctggacg gcgtcgaagt
     2761 ggtcatcctg cgcatgtcac agctcgagct cgtcgatgcc acgggggccc gcattctcaa
     2821 cgacatcgtc accacgctgg aacgacgcgg cgtcaccgta ctgatcaagg gcgtccggcc
     2881 cggccatcac gagctgttcc gcagcgtcgg ggtgctggac gcgctgcggc acgccgatca
     2941 cctcttcgat accctgccgc cggccatcga gcacgcccgc agccatgtgc gccgcgaacg
     3001 catcgagcgc tgggcccgag cccactagct cccactagct cagcgggtgc tgcggtctcg
     3061 gcctcgacca gctcgaccag cgggtcgcgc cgatcacccc tccagccgga cgcgcacctg
     3121 cacgctgcgg gcgttctgcc ggctcgccca ccagcacagc gccgcgagca actcgggatc
     3181 ctcgacgttg gtgctcagcc attcgccctc gacccgggcc cggtcggctc cccgcgacca
     3241 ctcgccccgc tccgtgaaca cctccgcggg cgccgcgcgt ccggccagca ggtcactgat
     3301 ccggtgcagt tccgggtcgc cggcgagccc ggcccgggac gcgagttcgt ccagcaaagc
     3361 gcggggggtg cgaaccgggt cgcggccgag cgacacgccc accctgcggg cggcctggac
     3421 ggtacccggt gcgatcgggt gcagccgacc gtggcggtct tcgatggtca acggttccag
     3481 cgcggtgacg aagccgatca ggtcccgggc ggggtcggtc agtcgcagtg tgacccgttc
     3541 gccgaccagc agcggcggta tcagcaagcg gtcggcatgc atgggctccc gttccggcaa
     3601 ggtgtgcgca gggtagagta ccgatgttcg agcagcgatc gaggagagtc gaagtgacgt
     3661 acatcatcac gcagccgtgt gtcgacgtga aagacctggc atgcgttgag gagtgtcccg
     3721 tggactgcat ctacgagggc aaccgcatgc tgtacatcca gcccgacgag tgcgtcgact
     3781 gcggtgcctg cgaacccgtc tgccccgtcg aggccatcta ctacgaagac gacgtacctg
     3841 ctgaccagaa ggtctactac gacgtcaacg tgaacttctt cgacaccgtc ggttcgcccg
     3901 gcggcgcggc ccgccagggt ccgatcgacg ccgaccaccc gtacgtggcc gcactgccgc
     3961 ctcagggcga gtgagccatc agggcgagtg agccttc
//
LOCUS       DAOSUG010000272         3994 bp    DNA     linear   ENV 10-JUN-2024
DEFINITION  MAG TPA_asm: Micropruina sp. isolate AMR_MDS_2195
            NODE_9431_length_3994_cov_3.996700, whole genome shotgun sequence.
ACCESSION   DAOSUG010000272 DAOSUG010000000
VERSION     DAOSUG010000272.1
DBLINK      BioProject: PRJNA925477
            BioSample: SAMN32864262
            Sequence Read Archive: SRR4236673
KEYWORDS    WGS; ENV; Metagenome Assembled Genome; MAG; Third Party Data; TPA;
            TPA:assembly.
SOURCE      Micropruina sp. (wastewater metagenome)
  ORGANISM  Micropruina sp.
            Bacteria; Bacillati; Actinomycetota; Actinomycetes;
            Propionibacteriales; Nocardioidaceae; Micropruina.
REFERENCE   1  (bases 1 to 3994)
  AUTHORS   Abdulkadir,N., Saraiva,J.P., Zhang,J., Stolte,S., Gillor,O.,
            Harms,H. and Rocha,U.
  TITLE     Genome-centric analyses of 165 metagenomes show that mobile genetic
            elements are crucial for the transmission of antimicrobial
            resistance genes to pathogens in activated sludge and wastewater
  JOURNAL   Microbiol Spectr 12 (3), e0291823 (2024)
   PUBMED   38289113
REFERENCE   2  (bases 1 to 3994)
  AUTHORS   Abdulkadir,N.
  TITLE     Direct Submission
  JOURNAL   Submitted (24-MAR-2023) Environmental Microbiology, Helmholtz
            Center for Environmental Research -UFZ, Permoserstr 15, Leipzig
            04318, Germany
COMMENT     The annotation was added by the NCBI Prokaryotic Genome Annotation
            Pipeline (PGAP). Information about PGAP can be found here:
            https://www.ncbi.nlm.nih.gov/genome/annotation_prok/
            
            ##Genome-Assembly-Data-START##
            Assembly Method        :: SPAdes v. 3.11.1
            Genome Representation  :: Full
            Expected Final Version :: Yes
            Genome Coverage        :: Not Applicable
            Sequencing Technology  :: Illumina MiSeq
            ##Genome-Assembly-Data-END##
            
            ##Genome-Annotation-Data-START##
            Annotation Provider               :: NCBI
            Annotation Date                   :: 05/04/2023 04:57:10
            Annotation Pipeline               :: NCBI Prokaryotic Genome
                                                 Annotation Pipeline (PGAP)
            Annotation Method                 :: Best-placed reference protein
                                                 set; GeneMarkS-2+
            Annotation Software revision      :: 6.5
            Features Annotated                :: Gene; CDS; rRNA; tRNA; ncRNA
            Genes (total)                     :: 2,776
            CDSs (total)                      :: 2,750
            Genes (coding)                    :: 2,714
            CDSs (with protein)               :: 2,714
            Genes (RNA)                       :: 26
            tRNAs                             :: 24
            ncRNAs                            :: 2
            Pseudo Genes (total)              :: 36
            CDSs (without protein)            :: 36
            Pseudo Genes (ambiguous residues) :: 0 of 36
            Pseudo Genes (frameshifted)       :: 2 of 36
            Pseudo Genes (incomplete)         :: 32 of 36
            Pseudo Genes (internal stop)      :: 2 of 36
            ##Genome-Annotation-Data-END##
FEATURES             Location/Qualifiers
     source          1..3994
                     /organism="Micropruina sp."
                     /mol_type="genomic DNA"
                     /submitter_seqid="NODE_9431_length_3994_cov_3.996700"
                     /isolate="AMR_MDS_2195"
                     /isolation_source="Activated sludge of municipal
                     wastewater treatment plants"
                     /db_xref="taxon:2737536"
                     /environmental_sample
                     /geo_loc_name="Canada"
                     /lat_lon="49.25 N 123.1 W"
                     /collection_date="2015-06-16"
                     /metagenome_source="wastewater metagenome"
                     /note="metagenomic"
     gene            complement(89..721)
                     /gene="pduL"
                     /locus_tag="PLF56_09295"
     CDS             complement(89..721)
                     /gene="pduL"
                     /locus_tag="PLF56_09295"
                     /EC_number="2.3.1.222"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_014847897.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="phosphate propanoyltransferase"
                     /protein_id="HOQ53794.1"
                     /translation="MPGTDSRQLVEEITAMVLTRLRFRIVLGVSNRHVHLSKEHLQTL
                     FGLDELTRFRQVRQPGDFAAEQFVAVHGPRTTFPKVRIMGPCRPKTQVELSRTDCIAL
                     GIDAPLTQSGHLDHAGPIDIEGPLGRIHLEHGAMVAARHIHMGPSHADQWGLKEQDRV
                     KVAFDGERGGVLDNLIIRLKDEWIPEIHLDTDEANALGLSDNDFGTMVRE"
     gene            complement(725..1003)
                     /locus_tag="PLF56_09300"
     CDS             complement(725..1003)
                     /locus_tag="PLF56_09300"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_013160816.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="BMC domain-containing protein"
                     /protein_id="HOQ53795.1"
                     /translation="MSEALGMVETKGYVGAVEAADAMVKSANVSLLGYQKVGAGLVTV
                     LVRGDVGAVKAAVDAGSAAAAAVGEEVVSKHVIPRPHSDVEQLLPKKP"
     gene            complement(1046..1588)
                     /locus_tag="PLF56_09305"
     CDS             complement(1046..1588)
                     /locus_tag="PLF56_09305"
                     /inference="COORDINATES: protein motif:HMM:NF013129.2"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="BMC domain-containing protein"
                     /protein_id="HOQ53796.1"
                     /translation="MSNLSLGTIEVLGLPAAVEAADVALKTANVQLIGYETTDGMGMV
                     AVKIEGQVSAVQSAVAAARAAAERITKVFAVSVIARPSDQLSPVVLTELTVGLEKPVE
                     PVGTHSAAPVDTASDETPSAPQAPGPDASAKPADVPADQADVPVEPADAPSGPPAVAS
                     STAATPTPARGRGRKAPAAP"
     gene            complement(1614..2033)
                     /locus_tag="PLF56_09310"
     CDS             complement(1614..2033)
                     /locus_tag="PLF56_09310"
                     /inference="COORDINATES: protein motif:HMM:NF014356.2"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="glycerol dehydratase reactivase beta/small
                     subunit family protein"
                     /protein_id="HOQ53797.1"
                     /translation="MTDERPTIRLRANDRLTDELFAEVLHGIEEEGVPVEVTRHAELN
                     PLVLAHQASLESRLGIGIGVSLDYVVITTEKLPEGRPYLAASLHRDPRIDRAAGANAA
                     RLVKRMPLHDLFAVQSSPNAGRDPSPSIHAGGSSATT"
     gene            complement(2030..3847)
                     /locus_tag="PLF56_09315"
     CDS             complement(2030..3847)
                     /locus_tag="PLF56_09315"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_013160813.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="diol dehydratase reactivase subunit alpha"
                     /protein_id="HOQ53798.1"
                     /translation="MALIAGVDIGNATTEVALAEVTPGAAPVFVASAIVSTTGVKGTA
                     QNTKGVFHALTSAALAAGRKVSDLDLIRINEAAPVIGDVAMETISETIITESTMIGHN
                     PTTPGGLGIGVGTTVLLAELAQAASGTPFIVVADRSYTFEDVARLINEAAARVSVTGV
                     ILQRDDGVLVHNRLRHKVPIVDEVSLIEKVPVGMLAAIEVADVGRIIETLANPYGIAT
                     LFGLDADETASVVPMARSLVGNRSAVVIKTPKGDVTERRIPAGHLSFIGDRTVEVDVE
                     RGADEIMRAAGSVGPIVNVKGQPGTNIGGMMEKVRVVMGRLTERDPSSIEVTDLLAVD
                     TNVPQKVTGGVAGEFSLEAAVGIAAMVKADRLQMQRIAHDMADQFGITVEVGGVEADM
                     AVRGALTTPGSSVPIAILDMGAGSTDASVLRADGKARSVHLAGAGNMVTLLIQSELGL
                     ESSEDAENVKRHPLARVETLFSIRHEDGTVQFFDEPLPPYTFARTVVLHPQEGMIPLP
                     LRQPLEVIRQVRIRAKERVFVTNALRALRLVSPTANVRDIAHVVLVGGSALDFEIPQL
                     VTRALGEYRVVAGRANIRGTEGPRNAVATGLVLAWEGAR"
BASE COUNT          543 a         1398 c         1388 g          665 t
ORIGIN      
        1 tcgaccacgc cggtgcgcac caggtgcgcg aaacggcgga tcgacgcctc gggggcgtcc
       61 agggatcgga tcggggtcat ggtggcagtc actcccgcac catcgttccg aagtcattgt
      121 cgctgaggcc gagcgcgttg gcctcgtcgg tgtcgaggtg gatctcggga atccactcgt
      181 ccttgagccg gatgatcagg ttgtcgagca cgccgccgcg ctcgccgtcg aaggccacct
      241 tgacccggtc ctgttccttg agtccccact ggtcggcgtg gctcgggccc atgtgaatgt
      301 gccgggccgc gaccatggcg ccgtgctcga ggtggatgcg tcccagcggc ccctcgatgt
      361 cgatcgggcc ggcatggtcg aggtgccccg actgggtcag cggcgcgtcg atgcccaggg
      421 cgatgcagtc ggtgcggctc agttcgacct gggtcttggg ccggcagggg cccatgatgc
      481 gcaccttggg aaacgtggtg cgcggcccgt gcacggcgac gaactgctcg gcggcgaagt
      541 caccgggctg gcgcacctgc cggaatcggg tcagctcgtc gagcccgaac agggtctgca
      601 gatgctcctt gctcaggtgc acgtgccggt tggacacccc gagcacgatc cgaaaccgca
      661 ggcgggtgag caccatcgca gtgatctcct cgacgagttg acgcgaatcc gtaccgggca
      721 tggctcaggg cttcttgggc agcaactgtt cgacgtcgct gtgcggccgg gggatcacgt
      781 gcttgctgac gacctcttcg ccgaccgccg cggccgcggc agagccggcg tccacggccg
      841 ccttcaccgc gccgacgtcg ccgcgcacca gcacggtgac caggcctgcg ccgaccttct
      901 ggtagccgag cagcgacacg ttcgccgact tgaccatggc gtcggccgct tcgacggcac
      961 cgacgtatcc cttcgtctcg accatgccga gcgcttcact catgttcttc tccttctgtg
     1021 gtggtggttg ggtgcggtgg ctggctcacg gtgccgcggg cgccttgcgg ccccgtccgc
     1081 gcgccggcgt aggggtcgcg gcggtggatg acgctacggc agggggcccg gacggcgcat
     1141 cggccggctc gaccgggacg tccgcctgat cggcagggac gtccgccggc ttcgccgatg
     1201 cgtccggtcc gggggcttgg ggtgcggacg gggtctcgtc cgacgcggtg tcgacgggcg
     1261 ctgcggagtg agtcccgacg ggttcgacgg gcttctccaa cccgacggtc agttcggtca
     1321 gcaccacagg tgacagctgg tcgctgggcc gggcgatcac cgagaccgcg aataccttgg
     1381 tgatcctctc ggccgcagcc cgggccgccg cgacggccga ctggacggcc gagacctggc
     1441 cctcgatctt gaccgccacc atgcccatgc cgtcggtggt ctcgtagccg atcagctgca
     1501 cgttggcggt cttgagcgcg acgtccgcgg cctcgacggc ggcgggcagg ccgagcacct
     1561 cgatggtgcc caggctcagg ttcgacatgg ttggctcctt cgggattctt cgctcaggtg
     1621 gtggcggacg atccgccggc gtgaatggac ggggacgggt cgcgacccgc gttgggcgat
     1681 gactgcaccg cgaacaggtc gtgcaacggc atgcgtttca ccaggcgcgc cgcgttggcc
     1741 ccggccgccc ggtcgatccg ggggtcgcgg tgcagtgacg cggccaggta cggacggcct
     1801 tccggcagct tttcggtggt gatcaccacg tagtcgaggc tgacgccgat gccgatgccg
     1861 agccgcgact ccaggctcgc ctggtgcgcc agcaccagcg ggttgagttc cgcgtgccgg
     1921 gtcacctcga ccggcacacc ctcctcctcg atgccgtgca gcacctcggc gaacagctcg
     1981 tcggtgagcc ggtcgttggc ccgcagcctg atggtgggtc gctcgtccgt catcgcgccc
     2041 cctcccacgc cagcaccagc ccggtcgcca ccgcgttgcg cgggccctcg gtgccacgga
     2101 tgttggcgcg tccggccacc acccggtact cccccagcgc cctggtcacc agttggggaa
     2161 tctcgaagtc gagcgcggac ccgccgacca gcaccacgtg ggcgatgtcg cggacgttcg
     2221 cggtcggact gaccaggcgc agcgcccgca gcgcgttggt gacgaagacc cgctccttgg
     2281 cccggatccg cacctgccgg atcacctcga gcggctgccg tagcggcagc gggatcatgc
     2341 cctcctgcgg gtgcagcacc acggtgcggg cgaaggtgta ggggggcagg ggctcgtcga
     2401 agaactgcac cgtgccgtcc tcgtgccgaa tgctgaacag ggtctcgacc cgggccagcg
     2461 gatgccgctt gacgttctcg gcgtcctcgg agctctccag ccccagctcg gactggatca
     2521 gcagcgtgac catgttgcct gcgccggcca gatgcaccga acgtgccttg ccgtcggctc
     2581 gcagcacgga ggcgtccgtc gatccggcgc ccatgtcgag aatcgcgatc ggcaccgacg
     2641 agccgggcgt ggtgagcgcg ccgcgcaccg ccatgtcggc ctcgaccccg ccgacctcga
     2701 cggtgatgcc gaactggtcg gccatgtcgt gggcgatgcg ctgcatctgc agccggtcgg
     2761 ccttcaccat agcggcgatg ccgaccgcgg cctcgagcga gaactcgccg gcgacgccgc
     2821 cggtgacctt ctgcggcacg ttggtgtcga cggccagcag gtcggtcacc tcgatgctgg
     2881 acgggtcgcg ctcggtcagc cgacccatca ccacccggac cttctccatc atgccgccga
     2941 tgttggtgcc gggctggccc ttgacgttga cgatcgggcc gaccgaaccg gccgcccgca
     3001 tgatctcgtc ggcaccccgc tcgacgtcga cctcgaccgt ccggtcaccg atgaaactga
     3061 ggtgccccgc aggaatgcgc cgctcggtga cgtcgccctt cggggtcttg atcaccaccg
     3121 ccgaccggtt gccgaccagc gagcgcgcca tcggcaccac cgaggcggtc tcgtcggcgt
     3181 ccagcccgaa cagggtggcg atgccgtacg ggttggccag ggtctcgatg atccggccga
     3241 cgtcggccac ctcgatggcg gccagcatgc cgaccggcac cttctcgatc aggctgacct
     3301 cgtcgacgat cggcaccttg tgccgcagcc ggttgtgcac cagcacgccg tcgtcgcgct
     3361 gcaggatcac gccggtcacg ctgacccggg cggccgcctc gttgatcagc cgggcgacgt
     3421 cctcaaaggt gtaggaccgg tcggcgacca cgatgaacgg ggtgccggag gcggcctggg
     3481 cgagttcggc gagcagcacc gtggtgccga cgccgatgcc cagaccgccc ggagtggtcg
     3541 ggttgtgccc gatcatggtc gactcggtga tgatggtctc cgagatcgtc tccatcgcca
     3601 catcgccgat caccggcgcc gcctcgttga tgcgaatcag atcgaggtcg ctcaccttcc
     3661 gtccggccgc cagggcggcc gaggtgagcg cgtgaaagac gcccttggtg ttctgggcag
     3721 tgcccttgac gccggtcgtc gacacgatcg cggacgccac gaacaccggg gccgcaccgg
     3781 gagtcacctc cgcgagtgcg acctcggtgg tcgcgttgcc gatatcgacg ccggctatca
     3841 gagccatgtg ggcgctctct ccttcgatgt gatcgtgcat tgtggacggg cctactccgt
     3901 caccccgggc gggcccggtt ccttcatccc gggcttgccc gcgtccttca acccgggcct
     3961 atccgtcatc ccgggcctat ccgtcatccc gggc
//
LOCUS       DAOSUG010000273         3993 bp    DNA     linear   ENV 10-JUN-2024
DEFINITION  MAG TPA_asm: Micropruina sp. isolate AMR_MDS_2195
            NODE_9434_length_3993_cov_3.810818, whole genome shotgun sequence.
ACCESSION   DAOSUG010000273 DAOSUG010000000
VERSION     DAOSUG010000273.1
DBLINK      BioProject: PRJNA925477
            BioSample: SAMN32864262
            Sequence Read Archive: SRR4236673
KEYWORDS    WGS; ENV; Metagenome Assembled Genome; MAG; Third Party Data; TPA;
            TPA:assembly.
SOURCE      Micropruina sp. (wastewater metagenome)
  ORGANISM  Micropruina sp.
            Bacteria; Bacillati; Actinomycetota; Actinomycetes;
            Propionibacteriales; Nocardioidaceae; Micropruina.
REFERENCE   1  (bases 1 to 3993)
  AUTHORS   Abdulkadir,N., Saraiva,J.P., Zhang,J., Stolte,S., Gillor,O.,
            Harms,H. and Rocha,U.
  TITLE     Genome-centric analyses of 165 metagenomes show that mobile genetic
            elements are crucial for the transmission of antimicrobial
            resistance genes to pathogens in activated sludge and wastewater
  JOURNAL   Microbiol Spectr 12 (3), e0291823 (2024)
   PUBMED   38289113
REFERENCE   2  (bases 1 to 3993)
  AUTHORS   Abdulkadir,N.
  TITLE     Direct Submission
  JOURNAL   Submitted (24-MAR-2023) Environmental Microbiology, Helmholtz
            Center for Environmental Research -UFZ, Permoserstr 15, Leipzig
            04318, Germany
COMMENT     The annotation was added by the NCBI Prokaryotic Genome Annotation
            Pipeline (PGAP). Information about PGAP can be found here:
            https://www.ncbi.nlm.nih.gov/genome/annotation_prok/
            
            ##Genome-Assembly-Data-START##
            Assembly Method        :: SPAdes v. 3.11.1
            Genome Representation  :: Full
            Expected Final Version :: Yes
            Genome Coverage        :: Not Applicable
            Sequencing Technology  :: Illumina MiSeq
            ##Genome-Assembly-Data-END##
            
            ##Genome-Annotation-Data-START##
            Annotation Provider               :: NCBI
            Annotation Date                   :: 05/04/2023 04:57:10
            Annotation Pipeline               :: NCBI Prokaryotic Genome
                                                 Annotation Pipeline (PGAP)
            Annotation Method                 :: Best-placed reference protein
                                                 set; GeneMarkS-2+
            Annotation Software revision      :: 6.5
            Features Annotated                :: Gene; CDS; rRNA; tRNA; ncRNA
            Genes (total)                     :: 2,776
            CDSs (total)                      :: 2,750
            Genes (coding)                    :: 2,714
            CDSs (with protein)               :: 2,714
            Genes (RNA)                       :: 26
            tRNAs                             :: 24
            ncRNAs                            :: 2
            Pseudo Genes (total)              :: 36
            CDSs (without protein)            :: 36
            Pseudo Genes (ambiguous residues) :: 0 of 36
            Pseudo Genes (frameshifted)       :: 2 of 36
            Pseudo Genes (incomplete)         :: 32 of 36
            Pseudo Genes (internal stop)      :: 2 of 36
            ##Genome-Annotation-Data-END##
FEATURES             Location/Qualifiers
     source          1..3993
                     /organism="Micropruina sp."
                     /mol_type="genomic DNA"
                     /submitter_seqid="NODE_9434_length_3993_cov_3.810818"
                     /isolate="AMR_MDS_2195"
                     /isolation_source="Activated sludge of municipal
                     wastewater treatment plants"
                     /db_xref="taxon:2737536"
                     /environmental_sample
                     /geo_loc_name="Canada"
                     /lat_lon="49.25 N 123.1 W"
                     /collection_date="2015-06-16"
                     /metagenome_source="wastewater metagenome"
                     /note="metagenomic"
     gene            complement(<1..471)
                     /locus_tag="PLF56_09320"
     CDS             complement(<1..471)
                     /locus_tag="PLF56_09320"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_016667380.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_function: GO:0003677 - DNA binding [Evidence IEA];
                     GO_process: GO:0000160 - phosphorelay signal transduction
                     system [Evidence IEA];
                     GO_process: GO:0006355 - regulation of DNA-templated
                     transcription [Evidence IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="response regulator transcription factor"
                     /protein_id="HOQ53799.1"
                     /translation="MGSDKQPSVLVVDDEPSLVRVVEGYLTKEGFSVRTAGDGETALA
                     LARESEPDVVVLDLGLPGIDGIEVCRQLRTFSSCYVLMLTARADEVDLLIGLAVGADD
                     YITKPFSARALVARVKTLLRRPRTPAGATAQEAPQARVFGDLTVDVPAREARVAG"
     gene            complement(474..1040)
                     /locus_tag="PLF56_09325"
     CDS             complement(474..1040)
                     /locus_tag="PLF56_09325"
                     /inference="COORDINATES: ab initio
                     prediction:GeneMarkS-2+"
                     /note="Derived by automated computational analysis using
                     gene prediction method: GeneMarkS-2+."
                     /codon_start=1
                     /transl_table=11
                     /product="hypothetical protein"
                     /protein_id="HOQ53800.1"
                     /translation="MMGANGRGWVGPSGGMMGAGGGSMMSGSMMGGGVWLAGDGVRVD
                     TITQARARATQAAAVSGLIPGEVMQFTNNFYVELRDASGEATTEVLVDPATGAVSTEY
                     GPAMMWNTGSRTAAISADEAAAIADRWLQANAAGEQAAGMAVAYPGYYTLDTASGDTT
                     VGMLSVNTTTGAVWYHTWHGRFVAEEDA"
     gene            complement(1205..1465)
                     /locus_tag="PLF56_09330"
     CDS             complement(1205..1465)
                     /locus_tag="PLF56_09330"
                     /inference="COORDINATES: ab initio
                     prediction:GeneMarkS-2+"
                     /note="Derived by automated computational analysis using
                     gene prediction method: GeneMarkS-2+."
                     /codon_start=1
                     /transl_table=11
                     /product="hypothetical protein"
                     /protein_id="HOQ53801.1"
                     /translation="MMGYGGFGGMGWGGMLGMVLLWAVLIGLVVWAVTRLLPTRPYGG
                     GMQPGPETPEEILDRRFARGEIDVETYQVQRAALASARGERR"
     gene            complement(1689..2555)
                     /locus_tag="PLF56_09335"
     CDS             complement(1689..2555)
                     /locus_tag="PLF56_09335"
                     /inference="COORDINATES: protein motif:HMM:NF021778.2"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="beta-propeller fold lactonase family protein"
                     /protein_id="HOQ53802.1"
                     /translation="MSPDGGTVWAVSGHANAVVALDAATYELLGTAPTGVSPAHVVVS
                     PDGEQVLVTNSGDDTLSVYDAESLELRATVDLSAGPHGLRPTPDGAAVVVANTTAGTV
                     DVVDTDTYEVTATIPVGDSPPQVAVAPDGRYAYVSLVGSSSVVKVDLVEQKVTGSVSV
                     PSPPVQLYLTADGTRLLSADQGTEGAPGSTVSVIDSASMTIEDTIAAGAGPHGVVVEP
                     TGQRAWVTNLYDDTVSVIDLSTNQTVSTASVGDKPNGISYSPQSPTPGPATTSVTVPD
                     YATGAEGGHAEH"
     gene            3100..3432
                     /locus_tag="PLF56_09340"
     CDS             3100..3432
                     /locus_tag="PLF56_09340"
                     /inference="COORDINATES: ab initio
                     prediction:GeneMarkS-2+"
                     /note="Derived by automated computational analysis using
                     gene prediction method: GeneMarkS-2+."
                     /codon_start=1
                     /transl_table=11
                     /product="hypothetical protein"
                     /protein_id="HOQ53803.1"
                     /translation="MATRRRRQLVTRDDVPLPPYEWSHDRSGAKSAWGVNLPISERHD
                     LQLMAQRGTAGRWTWTLTIYRTGSTDQVTRIARGVQPTLTAAKPAAEAAARTWLADQL
                     EHPDKSHD"
     gene            3494..>3993
                     /locus_tag="PLF56_09345"
     CDS             3494..>3993
                     /locus_tag="PLF56_09345"
                     /inference="COORDINATES: protein motif:HMM:NF014898.2"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_function: GO:0003677 - DNA binding [Evidence IEA];
                     GO_process: GO:0015074 - DNA integration [Evidence IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="site-specific integrase"
                     /protein_id="HOQ53804.1"
                     /translation="MLLPEDLSLDAAEAAFASASRASSTLRGYRSDWTEFVTWCRDNH
                     HTALPATDIAISRYISALATAGAKVGTISRRLSTIRFAHKVRNHPDPTQTARVIAVWE
                     GIRRQLGAPPDQARPLMPPLLLDVVDACPEMTKWKTRDDEPSVSGIRYRTLLLVGFVS
                     ALRASEL"
BASE COUNT          667 a         1469 c         1246 g          611 t
ORIGIN      
        1 gcccgcgacg cgcgcttcgc gcgccggaac gtccactgtc aggtccccga agacgcgtgc
       61 ctgcggggct tcctgtgccg tggcgccggc tggggtgcgt ggtcgccgta gcagcgtctt
      121 gacccgcgcg accagtgcgc gggcggagaa tggtttggtg atgtagtcgt cggctcccac
      181 tgcgagcccg atgagcaggt ccacctcatc ggcgcgcgca gtcagcatca ggacgtagca
      241 gctgctgaag gtccgcagct gccggcatac ctcgatgccg tcgatgccgg gcagccccag
      301 gtcgagcaca accacgtcgg gctctgattc ccgcgcgagt gcgagcgccg tctcgccgtc
      361 gccggcggtg cgcacggaga acccctcctt ggtcaggtag ccctccacca ccctgaccag
      421 gctgggttcg tcgtcaacca ccaggacgct cggctgcttg tcagacccca cgctcaagcg
      481 tcctcctccg cgacgaaccg accgtgccac gtgtggtacc agactgctcc ggtggtggtg
      541 ttgaccgaca gcatgcccac cgtcgtatcg cctgaggcgg tgtcgagggt gtagtagccc
      601 ggatacgcca cggccatgcc cgccgcctgc tcgccggcgg cgttggcctg cagccatcgg
      661 tcggcgatcg cggcggcctc atcagcagaa atggccgcgg tccggctgcc ggtgttccac
      721 atcatggccg gcccgtactc ggtggacacc gcgccggttg ccgggtccac gagcacctcg
      781 gtggtggcct caccggaggc gtccctcaac tcgacgtaga agttgttggt gaactgcatc
      841 acctcgcccg gtatgagccc actgaccgcc gcagcctgcg tcgcgcgagc ccttgcctgg
      901 gtgatggtgt cgacccggac gccgtcgccg gccagccaca ccccgccgcc catcatggag
      961 ccgctcatca tggagccgcc cccggcgccc atcattccgc ccgacggtcc gacccacccg
     1021 cgaccgttgg ctcccatcat cggaccatgc ccgtcccagt tcggcgcgac accggcagcg
     1081 ccagggtaag ggcgagcacc tccccaagcg gccaagacca ccgaggctat gaacagcacg
     1141 ctcgcgacca ccaacaccag ccacgccagc cagcgtgggc cggcctgagt caggccccgc
     1201 ccagtcatcg gcgttcaccc cgggccgagg ccagcgcagc ccgctgcacc tgatacgtct
     1261 cgacgtcaat ctcgccgcgg gcgaaccgac gatccaggat ctcctccggc gtctccggcc
     1321 cgggctgcat gccgccaccg taggggcgcg ttggcaagag cctggtcacc gcccacacca
     1381 ccagaccgat cagcaccgcc cacagcagca ccatgccgag catgccgccc caccccatcc
     1441 cgccaaaacc gccgtaaccc atcatgtcta tcctccagga ccgagtgggt ggtcccgtga
     1501 ccacccgatg caacaagctt gaccgtcacg tgtaaggatc ctcgcgcctg cggagtaaag
     1561 attccctcta catccatcca tgcatcggtt ccgtcacccc caatgtcgga tgtggaactg
     1621 cgggccgaga agcggctgcc atttcgagga ccgccccgtc gatcaggatg gagggtcgcc
     1681 cagccgggtc agtgctccgc gtggccgccc tcggctccgg tggcgtagtc ggggacggtg
     1741 acgctggtag tggcgggccc gggcgtaggc gattgagggc tgtagctgat gccgtttggc
     1801 ttgtcgccca cagacgcagt cgagacggtc tggttggtcg acaggtcgat cacggagacc
     1861 gtgtcgtcat agaggttggt cacccaggcc cgctgcccgg tcggttcgac aaccaccccg
     1921 tgcgggccag cccccgcggc gatggtgtcc tcgatggtca tggaggccga gtcgatcacg
     1981 gacacggtac tgccaggggc gccctcggtt ccctggtcgg ccgacagcaa gcgggtcccg
     2041 tccgcggtca ggtacagctg gaccggcggc gacggaaccg acaccgatcc cgtgaccttc
     2101 tgctccacca ggtcgacctt caccacagaa ctggacccga ccaggctcac gtaggcgtac
     2161 cgcccgtccg gtgcgacagc gacctgcgga ggggagtccc cgaccgggat cgtcgcggtg
     2221 acttcatagg tgtcagtgtc gaccacgtcg acagtgcccg ctgtggtgtt ggccacgacc
     2281 accgccgcac cgtccggggt gggacgcagg ccgtgcggcc cggcactgag gtcaacggtg
     2341 gcccggagct ccagcgactc ggcgtcgtag acgctcagcg tgtcatcccc cgagttggtg
     2401 acaaggacct gctcaccatc aggactaacg accacatgcg cagggctcac accggtcgga
     2461 gcggtcccca acagctcata cgtcgcggca tccagcgcca ccacagcgtt ggcgtgacca
     2521 ctgacagccc agaccgtgcc accatcaggg ctcacctgaa cattgtgagg actctcgatc
     2581 ccgctcaccg tggtcaccac gacaccggtg tccgcgtcga tggccgacaa ggaatcaccg
     2641 tcctcgttgg caacccacac cgtgccccgg atcgccatcg gctcctgagg tccggtgcct
     2701 ccctcaccgg cagcgccctc gtcgctctgc gtagcggcgg cactgctcaa cggcgcagcc
     2761 gaaccggcag ccggtgcatc ctgcccacag gcagtcagcg tcaaggcggc catcacaaag
     2821 acggtgagga acaaggggcg gcgcagggaa acgagcacgt gatttctcct aataacatgt
     2881 agaaatgtag gaccactatg acgcctcgtt cgtctgcctt cgatgctcat ccggtcaagg
     2941 ttccgtcaag gacacatcgc ctacccgggg aagtcccgcg aggcattggt tctcgctggg
     3001 cggcggcaag ccgctctacc tctgacgcac gtagatccac ccgcgtatta catcggatta
     3061 tccagagaat ccgatgtaat aaccgggtag gctcccggca tggccacacg acgccgacgg
     3121 caactcgtca cccgcgacga cgtgccgctg cccccgtacg agtggagcca cgaccgctcc
     3181 ggggcgaagt cggcgtgggg tgtgaacctg ccgatctctg agcgccacga tctgcagctg
     3241 atggcccagc gcggcaccgc cggccgctgg acctggacgc tcaccatcta ccgcaccggc
     3301 agcaccgacc aggtgacccg catcgcccgc ggcgtccagc ccaccctgac agccgccaaa
     3361 ccggccgccg aggccgccgc ccgcacctgg ctcgccgacc aactcgagca ccccgacaag
     3421 tcgcatgact gaaagccagc caggggccct gcacagtgcg gctggtggtc cggtcgccgc
     3481 tgatcatcag gcggtgctgc tgccggagga cctgtcgttg gatgcggccg aggccgcgtt
     3541 cgcctcggcg tcgcgggcct cgtcgacgct gcgcgggtac cgctccgact ggaccgagtt
     3601 cgtcacctgg tgccgcgaca accaccacac cgcgctgccc gccaccgaca tcgcgatctc
     3661 ccgctacatc tcggcgctgg ccaccgccgg ggcgaaggtc ggcacgatca gccggcgtct
     3721 gtcgacgatc cggttcgccc acaaggtccg taaccatccc gaccccaccc agaccgcacg
     3781 cgtgatcgcc gtctgggaag ggatccgccg gcagctcggg gctcccccgg accaggcccg
     3841 ccccctgatg cccccgctcc tcctcgacgt ggtcgatgcc tgcccggaga tgacgaaatg
     3901 gaagacccgc gacgatgaac cgtcagtgag cgggatccgg taccggacac tcctgctggt
     3961 cgggttcgtg tccgcgctgc gcgcctccga act
//
LOCUS       DAOSUG010000274         3985 bp    DNA     linear   ENV 10-JUN-2024
DEFINITION  MAG TPA_asm: Micropruina sp. isolate AMR_MDS_2195
            NODE_9446_length_3985_cov_3.994402, whole genome shotgun sequence.
ACCESSION   DAOSUG010000274 DAOSUG010000000
VERSION     DAOSUG010000274.1
DBLINK      BioProject: PRJNA925477
            BioSample: SAMN32864262
            Sequence Read Archive: SRR4236673
KEYWORDS    WGS; ENV; Metagenome Assembled Genome; MAG; Third Party Data; TPA;
            TPA:assembly.
SOURCE      Micropruina sp. (wastewater metagenome)
  ORGANISM  Micropruina sp.
            Bacteria; Bacillati; Actinomycetota; Actinomycetes;
            Propionibacteriales; Nocardioidaceae; Micropruina.
REFERENCE   1  (bases 1 to 3985)
  AUTHORS   Abdulkadir,N., Saraiva,J.P., Zhang,J., Stolte,S., Gillor,O.,
            Harms,H. and Rocha,U.
  TITLE     Genome-centric analyses of 165 metagenomes show that mobile genetic
            elements are crucial for the transmission of antimicrobial
            resistance genes to pathogens in activated sludge and wastewater
  JOURNAL   Microbiol Spectr 12 (3), e0291823 (2024)
   PUBMED   38289113
REFERENCE   2  (bases 1 to 3985)
  AUTHORS   Abdulkadir,N.
  TITLE     Direct Submission
  JOURNAL   Submitted (24-MAR-2023) Environmental Microbiology, Helmholtz
            Center for Environmental Research -UFZ, Permoserstr 15, Leipzig
            04318, Germany
COMMENT     The annotation was added by the NCBI Prokaryotic Genome Annotation
            Pipeline (PGAP). Information about PGAP can be found here:
            https://www.ncbi.nlm.nih.gov/genome/annotation_prok/
            
            ##Genome-Assembly-Data-START##
            Assembly Method        :: SPAdes v. 3.11.1
            Genome Representation  :: Full
            Expected Final Version :: Yes
            Genome Coverage        :: Not Applicable
            Sequencing Technology  :: Illumina MiSeq
            ##Genome-Assembly-Data-END##
            
            ##Genome-Annotation-Data-START##
            Annotation Provider               :: NCBI
            Annotation Date                   :: 05/04/2023 04:57:10
            Annotation Pipeline               :: NCBI Prokaryotic Genome
                                                 Annotation Pipeline (PGAP)
            Annotation Method                 :: Best-placed reference protein
                                                 set; GeneMarkS-2+
            Annotation Software revision      :: 6.5
            Features Annotated                :: Gene; CDS; rRNA; tRNA; ncRNA
            Genes (total)                     :: 2,776
            CDSs (total)                      :: 2,750
            Genes (coding)                    :: 2,714
            CDSs (with protein)               :: 2,714
            Genes (RNA)                       :: 26
            tRNAs                             :: 24
            ncRNAs                            :: 2
            Pseudo Genes (total)              :: 36
            CDSs (without protein)            :: 36
            Pseudo Genes (ambiguous residues) :: 0 of 36
            Pseudo Genes (frameshifted)       :: 2 of 36
            Pseudo Genes (incomplete)         :: 32 of 36
            Pseudo Genes (internal stop)      :: 2 of 36
            ##Genome-Annotation-Data-END##
FEATURES             Location/Qualifiers
     source          1..3985
                     /organism="Micropruina sp."
                     /mol_type="genomic DNA"
                     /submitter_seqid="NODE_9446_length_3985_cov_3.994402"
                     /isolate="AMR_MDS_2195"
                     /isolation_source="Activated sludge of municipal
                     wastewater treatment plants"
                     /db_xref="taxon:2737536"
                     /environmental_sample
                     /geo_loc_name="Canada"
                     /lat_lon="49.25 N 123.1 W"
                     /collection_date="2015-06-16"
                     /metagenome_source="wastewater metagenome"
                     /note="metagenomic"
     gene            <1..309
                     /locus_tag="PLF56_09350"
     CDS             <1..309
                     /locus_tag="PLF56_09350"
                     /inference="COORDINATES: protein motif:HMM:NF024854.2"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_function: GO:0003700 - DNA-binding transcription factor
                     activity [Evidence IEA];
                     GO_process: GO:0006355 - regulation of DNA-templated
                     transcription [Evidence IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="MarR family winged helix-turn-helix
                     transcriptional regulator"
                     /protein_id="HOQ53805.1"
                     /translation="RVGRLRDWIGWESSRLAHQLRRMERRGLVQRRPATDDARGTLVS
                     ITDEGRARIVAAAPGHVATVRANFIELMSPTERTVLAEVFERVARCINPDRGGLTSIG
                     "
     gene            370..1971
                     /locus_tag="PLF56_09355"
     CDS             370..1971
                     /locus_tag="PLF56_09355"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_002550870.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_function: GO:0005524 - ATP binding [Evidence IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="ABC-F family ATP-binding cassette
                     domain-containing protein"
                     /protein_id="HOQ53806.1"
                     /translation="MLVARDVEVRAGARLLLSPVSFQVSPGDKVGLVGRNGAGKTTLT
                     KILAGEALPAGGQITRQGQIGYLPQDPRTGDLDTLAKDRILSARGLDSVLRRLTRYSV
                     EMATETGEAQEQAMASYSRAEAELASAGGYAAESEAARIAANLGLPDRVLGQPLRTLS
                     GGQRRRVELARILFSGADNLLLDEPTNHLDADSIVWLRDFLKSYPGGVILISHDVGLL
                     DAIVTKVFHLDANRTELDQYAMDWKRYLLQRETDEKRRKRERQNAQRKAEQLLAQADK
                     MRAKATKATAAQNMAKRAERLLASVEGERAADRVAKIRFPTPAPCGKVPLRASDLSKS
                     YGSLEVFTDVTLAIDKGSRVVILGLNGAGKTTLLRILAGIETPDTGEVQPGHGLRLGY
                     YAQEHETLDVSRSVLENMMTAAPDDMTPTDARKILGSFLFTGDDVDKPARVLSGGEKT
                     RLALAMLVVSAANVLLLDEPTNNLDPASRAEVLGAIHSFTGAIVLVTHDEGAVEALEP
                     DRVLLLPDGDEDLWTPEYADLIALA"
     gene            complement(2182..3384)
                     /locus_tag="PLF56_09360"
     CDS             complement(2182..3384)
                     /locus_tag="PLF56_09360"
                     /inference="COORDINATES: protein motif:HMM:TIGR01891.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_function: GO:0004180 - carboxypeptidase activity
                     [Evidence IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="amidohydrolase"
                     /protein_id="HOQ53807.1"
                     /translation="MTSGWPNPRALALLDNLLAGIDDVLPQARALRRAIHADPYVGGL
                     EQPTADLLSAALNRRTTPLAGTGFWTRLGPDGPSVAVRSELDALPIVEHTDVEWAATN
                     GCMHACGHDVHMAAVWSLLKAAERIDLPIGLLGLFQPREECQPSGAPEVLASGILEQQ
                     DVRSVIGAHVQPRVPAGLVSSGIGGVNAAADAFDIVVRGYGGHGAYPYVTIDPVPILA
                     AIASGLYQLVGRLVDPTHAALISIGRLEAGAAHNIIPDSAKLQGIIRTMHESDRQMLH
                     AEVRRFAEHTAAARGATAEVSLLRGDPVLDNDHDLVVALDPVLRAAELPVAEEPFRSL
                     GADDFSHFGTRVPIVMMFVGTGEEADTRHDIGLHSARFLPGEHTIRTVAASLAAGYVA
                     GAHQAGAL"
     gene            complement(3429..>3985)
                     /locus_tag="PLF56_09365"
     CDS             complement(3429..>3985)
                     /locus_tag="PLF56_09365"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_002521376.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=3
                     /transl_table=11
                     /product="methyltransferase domain-containing protein"
                     /protein_id="HOQ53808.1"
                     /translation="ARLKQREVGAGTGHYLAELVEHRPPARGLALDVSRAAAKRAARA
                     HHRVASVVADVWRGLPVRSGAIDLVLCVFAPRNLAEFARVLRPDGRLLVVTPTVEHLA
                     GLRARYGLLGIDEDKHERLLAAASGRFELQHTQNHRLQTTISAAQVDDLIGMGPNAFH
                     HPPRADRAEQVQVSVQLSVLRPAA"
BASE COUNT          635 a         1326 c         1446 g          578 t
ORIGIN      
        1 cgggtgggca ggttgcggga ctggatcggc tgggagagca gccggctggc gcaccagttg
       61 cgccgcatgg aacggcgcgg cctggtgcag cgtcgtccgg ccaccgacga tgcgcgcggc
      121 acgttggtgt cgatcaccga cgaagggcgt gcgcggatcg tcgctgccgc ccccggtcac
      181 gtggccacgg tgcgcgccaa cttcatcgag ttgatgagcc cgaccgaacg taccgtgctc
      241 gccgaggtgt tcgaacgcgt ggcgaggtgc atcaaccccg atcgtggcgg tctgaccagc
      301 atcggctgaa ccgggcactg aagcgagcgc gttctgagcc tgccgctcgc ccgggtagag
      361 tggaccctcg tgctggttgc gagagatgtt gaggttcggg cgggtgcccg gttgctgctg
      421 tcgccggtct cgttccaggt ttcacccggt gacaaggtcg gattggtcgg acgtaacggg
      481 gccggcaaga ccacgctgac caagatcctg gccggtgagg cgttaccggc gggcggtcag
      541 atcacccgtc aagggcagat cggctacctg ccacaggacc cacgcaccgg cgacctcgac
      601 acgctcgcca aggaccgcat cctctcggcc cgcggactcg actccgtgct gcggcgactg
      661 acccgctact cggtcgagat ggccaccgaa accggtgagg cccaggaaca ggcgatggct
      721 tcatactcgc gggccgaggc cgagttggca tcggcaggcg ggtacgcggc cgaatccgag
      781 gcggcgcgga tcgccgccaa cctcggcctg ccggaccggg tgctcggcca gccgctgcgc
      841 accctctcgg gcggtcagcg gcgacgagtg gagttggccc gcattctgtt ctcgggggcc
      901 gacaacctgc tgctggacga gcccaccaac cacctggacg ccgactcgat cgtctggctg
      961 cgtgactttc tgaagtcgta cccgggcggg gtgatcctga tcagccacga cgtgggtttg
     1021 ttggacgcga tcgtcaccaa ggtctttcac ctggacgcca accgtaccga actcgaccag
     1081 tacgcgatgg actggaaacg ctacctgctg caacgtgaga ccgacgagaa gcgccgcaaa
     1141 cgcgaacgcc agaacgcgca gcgtaaggcc gagcagctgc tcgcgcaggc cgacaagatg
     1201 cgggccaagg ccaccaaggc cacggccgcg cagaacatgg cgaaacgggc cgagcggctg
     1261 ctggcctcgg tcgagggcga gcgggccgcc gatcgcgtgg cgaagatccg gttccccacc
     1321 ccggctccgt gcggcaaggt gccgctgcgg gcgtccgacc tgtcgaagag ctacggctcg
     1381 ctcgaggtgt tcaccgacgt cacgctggcc atcgacaagg gctcgcgggt ggtgatcctc
     1441 gggctgaacg gtgccggcaa gaccacgttg ctgcgcattc tggccggcat cgagaccccc
     1501 gacaccggtg aggtgcagcc cggacacggg ttgcgactgg gctattacgc gcaggagcac
     1561 gagaccctgg acgtgtcgcg cagcgtgctc gagaacatga tgaccgctgc ccccgacgac
     1621 atgacaccga ccgatgcgcg caagatcctg gggtcgttcc tgttcaccgg cgacgatgtg
     1681 gacaagccgg ccagggttct ctcgggtggc gagaaaaccc ggctggcatt ggcgatgctc
     1741 gtggtcagcg cggccaacgt gctgctgctg gacgagccga ccaacaacct cgacccggcg
     1801 tcacgggccg aggtgctggg ggcgattcat tcgttcacgg gcgcgatcgt gctggtcacc
     1861 cacgacgaag gcgcggtcga ggcgctggaa ccagatcggg tgctgctgct gccggacggt
     1921 gacgaagacc tctggacgcc cgagtatgcg gacctgatcg cactggctta gcccgaatcc
     1981 accgatggcc gccgtagcga gcggcaccag acgggtggcc tggcaagccg gaccgcgcga
     2041 gggcatgccg ggcgtctgtg gagcgcggcc ggtgcagtca gggcgcccgg atggggccgc
     2101 gcagtaggcg gagatcggtg gattggggct taggacgtgc tgatcgatac gcaaccaacg
     2161 gatcgcgcag ggatccacgc cctagagcgc tccggcctga tgggcgccgg ccacgtagcc
     2221 ggcggccagg ctggccgcca ccgtacgaat ggtgtgctcg ccgggtagga accgtgccga
     2281 atgcaggccg atgtcgtgcc gggtgtcggc ctcttcgccg gtgccgacga acatcatcac
     2341 gatcggcaca cgggtgccga agtggctgaa gtcgtcggcg ccgagcgagc ggaacggctc
     2401 ttcggccacc ggcaactcgg cggcacgcag cacgggatcg agtgcgacca ccagatcgtg
     2461 gtcgttgtcg agcaccgggt cgccgcgcag caggctgacc tcggcggtcg ctccccgggc
     2521 cgcggcggtg tgctcggcga atcggcgcac ctcggcgtgc aacatctgcc ggtccgactc
     2581 gtgcatggtg cggatgatgc cctgcagctt cgccgagtcg ggaatgatgt tgtgggcggc
     2641 accggcttcg agccgtccga tgctgatcag ggcggcgtgg gtggggtcga cgagtcgtcc
     2701 gaccagctgg tacagcccgg acgcgatcgc ggccaggatc ggcaccggat cgatcgtcac
     2761 gtacgggtag gccccgtggc cgccgtagcc gcgcaccacg atgtcgaagg cgtcggcggc
     2821 tgcgttgacg ccgccgatgc cgctgctgac caggcccgcg ggcacccggg gctgcacgtg
     2881 cgcgccgatc accgagcgga cgtcctgctg ttcgagaatg ccgctggcca gcacctcggg
     2941 ggcgcccgag ggctgacact cctcgcgtgg ctgaaagagg ccgagcaggc cgatcggcag
     3001 gtcgatccgt tcggctgcct tgagcaagga ccacaccgcc gccatgtgca cgtcatgccc
     3061 acaggcgtgc atgcacccgt tcgtcgcggc ccactcgacg tcggtgtgct cgacgatcgg
     3121 cagtgcgtcc agctcgctgc gcaccgcgac ggaggggccg tcggggccga ggcgcgtcca
     3181 gaaaccggtg ccggccagcg gggtggtgcg acgattcagc gccgcgctga gcaggtcggc
     3241 ggtgggctgc tccagcccac ccacgtacgg gtcggcgtga atggcccgac gcagcgcccg
     3301 agcctgcggc aacacgtcat cgatgccggc cagcaggttg tcgagaagtg cgagcgcgcg
     3361 cgggttcggc cagccagaag tcacaaccgg gcagtgtagt gcggggtggc gccgcccctg
     3421 acgcgcgttc atgccgccgg acgcaacacg ctgagctgca cggacacctg cacctgctcg
     3481 gcccggtcgg cccggggcgg atggtggaag gcgttgggac ccatgccgat caggtcgtcg
     3541 acctgggcgg ccgagatcgt ggtctgcagc cggtggttct gcgtgtgctg cagctcgaac
     3601 cgtccggacg ccgccgcgag tagccgctcg tgcttgtcct cgtcgattcc gagcaggccg
     3661 taacgagcgc gcagtccggc cagatgctcc acggtcggcg tgaccaccag caaccgtccg
     3721 tcggggcgca gcacccgcgc gaactcggcc aggttgcggg gggcgaacac gcacagcacc
     3781 aggtcgatcg ccccgctgcg caccgggagt ccgcgccaga cgtccgcgac cacggaggcg
     3841 acccggtgat gggcgcgggc cgcacgcttt gccgccgccc gcgaaacgtc cagggcaagg
     3901 ccccgggccg gcggacgatg ctcgaccagt tcggccaggt agtggcccgt gcccgcgccc
     3961 acctcacgct gcttgaggcg ggcgc
//
LOCUS       DAOSUG010000275         3961 bp    DNA     linear   ENV 10-JUN-2024
DEFINITION  MAG TPA_asm: Micropruina sp. isolate AMR_MDS_2195
            NODE_9512_length_3961_cov_3.546083, whole genome shotgun sequence.
ACCESSION   DAOSUG010000275 DAOSUG010000000
VERSION     DAOSUG010000275.1
DBLINK      BioProject: PRJNA925477
            BioSample: SAMN32864262
            Sequence Read Archive: SRR4236673
KEYWORDS    WGS; ENV; Metagenome Assembled Genome; MAG; Third Party Data; TPA;
            TPA:assembly.
SOURCE      Micropruina sp. (wastewater metagenome)
  ORGANISM  Micropruina sp.
            Bacteria; Bacillati; Actinomycetota; Actinomycetes;
            Propionibacteriales; Nocardioidaceae; Micropruina.
REFERENCE   1  (bases 1 to 3961)
  AUTHORS   Abdulkadir,N., Saraiva,J.P., Zhang,J., Stolte,S., Gillor,O.,
            Harms,H. and Rocha,U.
  TITLE     Genome-centric analyses of 165 metagenomes show that mobile genetic
            elements are crucial for the transmission of antimicrobial
            resistance genes to pathogens in activated sludge and wastewater
  JOURNAL   Microbiol Spectr 12 (3), e0291823 (2024)
   PUBMED   38289113
REFERENCE   2  (bases 1 to 3961)
  AUTHORS   Abdulkadir,N.
  TITLE     Direct Submission
  JOURNAL   Submitted (24-MAR-2023) Environmental Microbiology, Helmholtz
            Center for Environmental Research -UFZ, Permoserstr 15, Leipzig
            04318, Germany
COMMENT     The annotation was added by the NCBI Prokaryotic Genome Annotation
            Pipeline (PGAP). Information about PGAP can be found here:
            https://www.ncbi.nlm.nih.gov/genome/annotation_prok/
            
            ##Genome-Assembly-Data-START##
            Assembly Method        :: SPAdes v. 3.11.1
            Genome Representation  :: Full
            Expected Final Version :: Yes
            Genome Coverage        :: Not Applicable
            Sequencing Technology  :: Illumina MiSeq
            ##Genome-Assembly-Data-END##
            
            ##Genome-Annotation-Data-START##
            Annotation Provider               :: NCBI
            Annotation Date                   :: 05/04/2023 04:57:10
            Annotation Pipeline               :: NCBI Prokaryotic Genome
                                                 Annotation Pipeline (PGAP)
            Annotation Method                 :: Best-placed reference protein
                                                 set; GeneMarkS-2+
            Annotation Software revision      :: 6.5
            Features Annotated                :: Gene; CDS; rRNA; tRNA; ncRNA
            Genes (total)                     :: 2,776
            CDSs (total)                      :: 2,750
            Genes (coding)                    :: 2,714
            CDSs (with protein)               :: 2,714
            Genes (RNA)                       :: 26
            tRNAs                             :: 24
            ncRNAs                            :: 2
            Pseudo Genes (total)              :: 36
            CDSs (without protein)            :: 36
            Pseudo Genes (ambiguous residues) :: 0 of 36
            Pseudo Genes (frameshifted)       :: 2 of 36
            Pseudo Genes (incomplete)         :: 32 of 36
            Pseudo Genes (internal stop)      :: 2 of 36
            ##Genome-Annotation-Data-END##
FEATURES             Location/Qualifiers
     source          1..3961
                     /organism="Micropruina sp."
                     /mol_type="genomic DNA"
                     /submitter_seqid="NODE_9512_length_3961_cov_3.546083"
                     /isolate="AMR_MDS_2195"
                     /isolation_source="Activated sludge of municipal
                     wastewater treatment plants"
                     /db_xref="taxon:2737536"
                     /environmental_sample
                     /geo_loc_name="Canada"
                     /lat_lon="49.25 N 123.1 W"
                     /collection_date="2015-06-16"
                     /metagenome_source="wastewater metagenome"
                     /note="metagenomic"
     gene            <1..1134
                     /locus_tag="PLF56_09370"
     CDS             <1..1134
                     /locus_tag="PLF56_09370"
                     /inference="COORDINATES: ab initio
                     prediction:GeneMarkS-2+"
                     /note="binding of PriA to forked DNA starts the assembly
                     of the primosome, also possesses 3'-5' helicase activity;
                     Derived by automated computational analysis using gene
                     prediction method: GeneMarkS-2+."
                     /codon_start=1
                     /transl_table=11
                     /product="primosome assembly protein PriA"
                     /protein_id="HOQ53809.1"
                     /translation="APYPHARVVAALRSARQHCGLLFVGYARTAETESLVDRGWLVSL
                     QAPASARREVCPVVRVASATEHAAERDPAAHSRLPHEVFSAIRAGLSGGPVLVQVPRA
                     GYLAALACGRCRAIARCPACGLPLTGEQVTQGTGVVCRVCGLHPEWRCPECDSTRLRA
                     PRVGVARTAEELGRAFPHTRVVQSSGEKRVAEVGIEPALVLATPGAEPVASEGYAAAV
                     LLDADLLLARADLRATEEALRRWLAAVALVRGAGDDGTVVLVGDPGARAVQALVRLDP
                     IGFAERELHDRAEAGFAPATKLVGLEGTHEAVIEATRVLQADTGAGVLGPVPAAEGEF
                     RALLRAPLDRGQALVAAAKAVQAQRTAHKDDGSLRVSVDPAVV"
     gene            1308..2228
                     /gene="fmt"
                     /locus_tag="PLF56_09375"
     CDS             1308..2228
                     /gene="fmt"
                     /locus_tag="PLF56_09375"
                     /EC_number="2.1.2.9"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_013863510.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_component: GO:0005737 - cytoplasm [Evidence IEA];
                     GO_function: GO:0004479 - methionyl-tRNA formyltransferase
                     activity [Evidence IEA];
                     GO_process: GO:0006431 - methionyl-tRNA aminoacylation
                     [Evidence IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="methionyl-tRNA formyltransferase"
                     /protein_id="HOQ53810.1"
                     /translation="MRVVFAGTPEVALPTLNALAASRHELVAVISRPDAPQGRSKRLV
                     PSPVATWATQHDIETLKPAKAGEPDFVRRLSELRPDCCPVVAYGALIPAPVLAIPRHG
                     WVNVHFSLLPAWRGAAPVQRAILAGDTETGISIFDLVPALDAGPVYLREPSPLGDAET
                     AGGVLARLADRGADLLVDVLDAIENGTAVASPQPDERITLAPKLTAADARIDWTGPAI
                     SIDRQIRACNPNPVAWTTHRDERVKVWLARSHPGTVLAPGMLRVDKRSATVGTGRGVL
                     ELLTVQPAGKRAMDAAAWARGLGADPGALE"
     gene            2497..3831
                     /locus_tag="PLF56_09380"
     CDS             2497..3831
                     /locus_tag="PLF56_09380"
                     /EC_number="2.1.1.-"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_016669874.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_function: GO:0003723 - RNA binding [Evidence IEA];
                     GO_function: GO:0008168 - methyltransferase activity
                     [Evidence IEA];
                     GO_function: GO:1904047 - S-adenosyl-L-methionine binding
                     [Evidence IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="RsmB/NOP family class I SAM-dependent RNA
                     methyltransferase"
                     /protein_id="HOQ53811.1"
                     /translation="MSANRGRRGTPADPARLAAFRTMRAITADDAYANLALAEHTTGL
                     SPRDAAFATELVAGTCRSLGLYDAIVTSASGRELKTLQPAVVDLLRLGTHQLLELRVP
                     SHAAISATVDLARAAVSDRVTGVVNAILRKVAALDADGWAARLRTERPDADALIARHP
                     QWIIDALADVLPAEELDDALAADNVAPTPHLVVRPGLAEIAELGGEPLRWSPFGARAA
                     GNPADVPAVAQGRAGVQDEGSQLVAWALARIDAPDGPWLDLCAGPGGKAALLAGLARD
                     QGERLLAAELQPHRAALVARALAAYPQPRPLVIAADGTRPAWPPASFARVMLDAPCSG
                     LGALRRRPESRWRRQPSDLDVLVPLQRALLRNAIAAAVPGGVIAYVTCSPHQRETVQV
                     VEAADGVEVIDASSVLPEVPDAARGPYLQVWPHRHGTDAMFLALLRKTPSTQ"
     gene            3842..>3961
                     /locus_tag="PLF56_09385"
     CDS             3842..>3961
                     /locus_tag="PLF56_09385"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_002545254.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="ribulose-phosphate 3-epimerase"
                     /protein_id="HOQ53812.1"
                     /translation="MTMRITPSILNADLADLSGEIGRIPSADGVHIDVMDNRFV"
BASE COUNT          524 a         1407 c         1458 g          572 t
ORIGIN      
        1 gccccgtacc cgcatgcccg ggtggtggcg gcgctgcggt cggctcggca gcattgcggg
       61 ctacttttcg tcgggtatgc ccgtaccgcc gagaccgagt cgctggtcga tcgaggctgg
      121 ttggtgtcgt tgcaggcccc agcctcagcg cgccgtgagg tgtgcccggt ggtgcgggtc
      181 gcgtcggcga cggagcatgc ggccgaacgc gaccccgcgg cgcattccag attgccccac
      241 gaggtgttca gcgcgatcag ggccggcctg tccggcgggc cggtgctggt gcaggttccg
      301 cgggcggggt acctcgccgc attggcgtgt ggccggtgcc gcgcgatcgc tcggtgtccc
      361 gcgtgcgggc tcccgttgac cggcgaacag gtgactcagg gcactggtgt ggtgtgccgt
      421 gtctgcgggc tgcatcccga atggcgctgc cccgagtgtg acagtacgcg gcttcgcgcc
      481 ccccgggtcg gcgttgcgcg cactgccgaa gaactcggac gggcctttcc gcacacccgc
      541 gtggtgcaat cgagcggcga gaagcgggtg gccgaggtgg gtatcgagcc cgcattggtg
      601 ctggccacgc ctggtgccga gccggtggcg agcgagggct atgcggcagc ggtgctgctc
      661 gatgccgacc tgctgctggc ccgggccgac ctgcgcgcga ccgaagaggc gctgcggcgc
      721 tggttggcgg cggtcgcgct ggttcgcggc gcgggcgatg atggcacggt ggtgctggtc
      781 ggcgatccgg gtgcgagggc cgtccaggcg ctggtgcggc tcgaccccat cggcttcgcc
      841 gaacgtgaac tccacgaccg ggccgaggcc gggttcgcac ccgcaaccaa gctggtcggc
      901 ctcgaaggca cgcacgaggc ggtgatcgaa gcgacccgag tgctgcaggc cgacacgggc
      961 gccggggtgt tgggtccggt gccggcggcc gagggtgagt tccgcgccct gctccgtgca
     1021 cccctcgacc gtggccaggc cctggtggcc gcggctaagg cggtgcaagc tcagcgcacc
     1081 gcccacaaag acgacgggtc gctgcgggtg agtgtcgacc cggcggtggt ctgagagcgc
     1141 tcaggcgttc ggctcacgcg tgcgcccggc cgccgaacgc ccaccgaact atctcccgcc
     1201 tgcgaagctt cgcaggccag agatggttag gcgggcgttg gacgcggctg tccgatggcc
     1261 ctcttctgct cacggtggcc ttcgtgacgg ttcgctaggc tgacgcggtg cgcgtcgtct
     1321 tcgccggaac ccccgaggtt gccctgccca ccctgaacgc gctggccgcc tcacgacacg
     1381 aactggtggc tgtgatcagc cgtccggacg caccgcaggg acgcagcaag cggctcgtcc
     1441 cgagcccggt ggcaacctgg gccacccagc atgacatcga gaccctgaag cccgccaaag
     1501 cgggtgagcc cgacttcgtc cggcggctga gcgaactgcg gcccgattgc tgtccggtcg
     1561 tcgcctacgg ggcactcatc cccgcgcccg tgctggcgat tccgcgccac ggctgggtga
     1621 acgtgcattt ttcgctgctg cccgcctggc gaggcgcggc tccggtgcag cgggcgattc
     1681 tggccggcga caccgagacc ggcatcagca tcttcgacct ggtgcccgca ttggacgccg
     1741 ggccggtcta cctgcgcgag ccctcgccgc tgggcgacgc cgagaccgca ggcggagtgc
     1801 tggcgcggct ggccgatcgc ggcgccgacc tcctggtgga cgtgctggac gcgatcgaga
     1861 acggtaccgc ggtggcatcg ccgcagccgg acgaacggat cacgctcgcc cccaagctga
     1921 ccgccgccga cgcgcgcatc gactggacgg ggcccgcgat ctccattgac cgccagatcc
     1981 gggcctgcaa ccccaacccg gtcgcctgga ccacgcaccg cgacgagcgg gtcaaggtat
     2041 ggctggcacg gtcccacccg ggcaccgtgc tggccccggg catgttgcgg gtggacaagc
     2101 gcagcgcgac cgtcggcacc gggcgaggcg tcctggaact gctcacggtg cagccggccg
     2161 gaaagagagc catggatgcg gccgcctggg cgcgcggcct gggcgccgac cccggggcac
     2221 tcgagtgagt gggcgtcggg gcgtgtcggc gcaggtaggg gtctcgatca cctcgaccag
     2281 cgggaatcgt tcgaccagcg ggaatcgttc gaccagcgcg gtctctacca gcgcgaccag
     2341 cgggagccgt tcgaccagcg ggcggtctcg acaggctcga ccagcgagga tggctcgacc
     2401 cgcgatggcc gttcgagcgg cggaggggcc cggtcaggcg cgaccagcgg gggcgcaccg
     2461 aacagcggaa acgccccggc cggcgggggg ctctgcatga gcgccaaccg tgggcgcagg
     2521 gggacgccgg ccgacccggc ccgcttggcg gctttccgca ccatgcgcgc gatcaccgct
     2581 gacgacgcct acgccaacct tgcgctcgcc gaacacacca cgggcctgtc gcccagggac
     2641 gctgccttcg ccacggagct ggtcgccggc acctgccggt cgctcggcct gtacgacgcg
     2701 atcgtcacgt ctgcctcggg ccgtgagctc aagaccctcc aaccggcagt cgtcgacctc
     2761 ttgcgcctcg gcacccacca gttgctggag ttgcgggtgc cgagccacgc ggcgatctcg
     2821 gccaccgtcg acctggcccg tgccgcggtg tccgaccggg tcaccggcgt ggtcaatgcg
     2881 atcctccgca aggtggccgc cctggacgcg gacggctggg ccgctcgact gcgcaccgag
     2941 cgtccggacg ccgacgcgct gatcgccagg cacccgcagt ggatcatcga cgccctcgcc
     3001 gacgtgctac ccgctgagga actggacgac gccctggccg ccgacaacgt cgctccgacg
     3061 ccgcatctgg tggtccgccc ggggctggcc gaaattgccg aactcggcgg tgaacccctg
     3121 cgctggtcgc cgttcggcgc ccgggccgcg ggcaaccccg ccgatgtgcc ggccgtcgcg
     3181 caggggcgcg ccggggttca ggacgagggc tcgcagctgg tggcctgggc gctggcccgc
     3241 atcgacgcac cggacggtcc gtggctcgac ctctgcgccg gtcccggcgg caaggccgcg
     3301 ctgctcgccg gactggcccg tgaccagggc gaacgcttgc tggccgcgga actgcaaccc
     3361 caccgtgccg cgctcgtggc aagggcgttg gcggcctacc cgcaaccacg tccgctcgtg
     3421 atcgccgccg acggcaccag gcccgcctgg ccgccggcga gcttcgcccg ggtgatgctg
     3481 gacgccccct gcagcggcct gggtgcgttg cgacggcgtc cggagtcgcg atggcggcgg
     3541 cagccgtccg acctggacgt tctggtgccc ctgcaacggg cgctgctgcg caacgcgatc
     3601 gcggcggcgg tccctggtgg cgtgatcgcc tacgtgacct gctcgccgca tcagcgcgag
     3661 accgtccagg tcgtagaggc ggccgacggt gtcgaggtga tcgacgcctc gtcggtgctg
     3721 cccgaggtgc cggacgccgc tcgcggcccc tacctgcagg tgtggccgca ccggcacggc
     3781 accgacgcga tgttcctggc gctgctacgc aagacgcctt cgacccaata gggtcgatcg
     3841 catgacgatg cggatcacac cgagcattct gaacgccgac ctggccgatc tcagtggcga
     3901 gatcggccgc attcccagcg cggacggcgt gcacatcgac gtgatggaca accggttcgt
     3961 g
//
LOCUS       DAOSUG010000276         3957 bp    DNA     linear   ENV 10-JUN-2024
DEFINITION  MAG TPA_asm: Micropruina sp. isolate AMR_MDS_2195
            NODE_9518_length_3957_cov_4.481292, whole genome shotgun sequence.
ACCESSION   DAOSUG010000276 DAOSUG010000000
VERSION     DAOSUG010000276.1
DBLINK      BioProject: PRJNA925477
            BioSample: SAMN32864262
            Sequence Read Archive: SRR4236673
KEYWORDS    WGS; ENV; Metagenome Assembled Genome; MAG; Third Party Data; TPA;
            TPA:assembly.
SOURCE      Micropruina sp. (wastewater metagenome)
  ORGANISM  Micropruina sp.
            Bacteria; Bacillati; Actinomycetota; Actinomycetes;
            Propionibacteriales; Nocardioidaceae; Micropruina.
REFERENCE   1  (bases 1 to 3957)
  AUTHORS   Abdulkadir,N., Saraiva,J.P., Zhang,J., Stolte,S., Gillor,O.,
            Harms,H. and Rocha,U.
  TITLE     Genome-centric analyses of 165 metagenomes show that mobile genetic
            elements are crucial for the transmission of antimicrobial
            resistance genes to pathogens in activated sludge and wastewater
  JOURNAL   Microbiol Spectr 12 (3), e0291823 (2024)
   PUBMED   38289113
REFERENCE   2  (bases 1 to 3957)
  AUTHORS   Abdulkadir,N.
  TITLE     Direct Submission
  JOURNAL   Submitted (24-MAR-2023) Environmental Microbiology, Helmholtz
            Center for Environmental Research -UFZ, Permoserstr 15, Leipzig
            04318, Germany
COMMENT     The annotation was added by the NCBI Prokaryotic Genome Annotation
            Pipeline (PGAP). Information about PGAP can be found here:
            https://www.ncbi.nlm.nih.gov/genome/annotation_prok/
            
            ##Genome-Assembly-Data-START##
            Assembly Method        :: SPAdes v. 3.11.1
            Genome Representation  :: Full
            Expected Final Version :: Yes
            Genome Coverage        :: Not Applicable
            Sequencing Technology  :: Illumina MiSeq
            ##Genome-Assembly-Data-END##
            
            ##Genome-Annotation-Data-START##
            Annotation Provider               :: NCBI
            Annotation Date                   :: 05/04/2023 04:57:10
            Annotation Pipeline               :: NCBI Prokaryotic Genome
                                                 Annotation Pipeline (PGAP)
            Annotation Method                 :: Best-placed reference protein
                                                 set; GeneMarkS-2+
            Annotation Software revision      :: 6.5
            Features Annotated                :: Gene; CDS; rRNA; tRNA; ncRNA
            Genes (total)                     :: 2,776
            CDSs (total)                      :: 2,750
            Genes (coding)                    :: 2,714
            CDSs (with protein)               :: 2,714
            Genes (RNA)                       :: 26
            tRNAs                             :: 24
            ncRNAs                            :: 2
            Pseudo Genes (total)              :: 36
            CDSs (without protein)            :: 36
            Pseudo Genes (ambiguous residues) :: 0 of 36
            Pseudo Genes (frameshifted)       :: 2 of 36
            Pseudo Genes (incomplete)         :: 32 of 36
            Pseudo Genes (internal stop)      :: 2 of 36
            ##Genome-Annotation-Data-END##
FEATURES             Location/Qualifiers
     source          1..3957
                     /organism="Micropruina sp."
                     /mol_type="genomic DNA"
                     /submitter_seqid="NODE_9518_length_3957_cov_4.481292"
                     /isolate="AMR_MDS_2195"
                     /isolation_source="Activated sludge of municipal
                     wastewater treatment plants"
                     /db_xref="taxon:2737536"
                     /environmental_sample
                     /geo_loc_name="Canada"
                     /lat_lon="49.25 N 123.1 W"
                     /collection_date="2015-06-16"
                     /metagenome_source="wastewater metagenome"
                     /note="metagenomic"
     gene            217..1290
                     /gene="ychF"
                     /locus_tag="PLF56_09390"
     CDS             217..1290
                     /gene="ychF"
                     /locus_tag="PLF56_09390"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_013865208.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_function: GO:0005525 - GTP binding [Evidence IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="redox-regulated ATPase YchF"
                     /protein_id="HOQ53813.1"
                     /translation="MALTIGIVGLPNAGKSTLFNALTRNEVLAANYPFATIEPNVGVV
                     GVPDERLPKLAKLFKSAQIIPATVSFVDIAGIVKGASQGEGMGNAFLANIREADAICQ
                     VTRVFRDPDVTHVDGAVNPGSDIDTIGTELILADLQTIERALPRLEKEARLHKDRKVV
                     LEAVQQAQQVLDSGRGVQAAGLDLEPLKELFLLTAKPYIYVFNCDSDELTDEPLLAGM
                     RELVAPAEAIFLDAKIESELVEMEPDEAREFLEEMGIDEPGLDTLARVGYETLGLQSY
                     LTAGPKESRAWTIRKGATAPEAAGVIHTDFQKGFIKAEIVSYDDLVRLGGMQQAKAAG
                     KVRLEGKDYVMADGDVVEFRFST"
     gene            1363..1587
                     /locus_tag="PLF56_09395"
     CDS             1363..1587
                     /locus_tag="PLF56_09395"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_012923800.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_process: GO:0006355 - regulation of DNA-templated
                     transcription [Evidence IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="ribbon-helix-helix protein, CopG family"
                     /protein_id="HOQ53814.1"
                     /translation="MTNVLIRGLSEAAVERIDAEAAALGLSRNEFLRRRLEKEAVAPL
                     AATITADDWRRSAAAFGDLTDPDVMDAAWR"
     gene            1578..1997
                     /locus_tag="PLF56_09400"
     CDS             1578..1997
                     /locus_tag="PLF56_09400"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_013865206.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="PIN domain nuclease"
                     /protein_id="HOQ53815.1"
                     /translation="MAVTSWLVDKSAYVRMQSGQAAQLQEWNGRIERGLMHLSAVTRL
                     ELGFSARSGAEGRDAFGLPPLSLMPIEHLTPAIEDRAWELQMMLADRGQHRAPSIPDL
                     LIAATAEKTGLTVLAIDKDFDLIAEITGQPVQTLAVR"
     gene            2025..3254
                     /locus_tag="PLF56_09405"
     CDS             2025..3254
                     /locus_tag="PLF56_09405"
                     /inference="COORDINATES: protein motif:HMM:NF039738.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="DUF6357 family protein"
                     /protein_id="HOQ53816.1"
                     /translation="MSEVIFARDDQWLPRVVREEGGLRAAVVGGADALHGPRVFSFPI
                     EEAHVAALRVDLARHLILDSALRPLCDDAGTRGTLDEHAAVALLDPILLASPDDVDAF
                     LRHTRWDRSALVARGANRALLDRGRVMAALGAADGRADWSRAQQDTADRDRSRRGVTL
                     SALDAAVLRFTGQYLHAATVPRRDPAAVDAELLPQVQQVIATAEQACAGIGIARDPRR
                     GPRGTDKADWRRLESIVQQALGAAHPRLGADAVGSVSFLICSEAAGRALLGPYDTDDS
                     AAPAPRRTLTFTDDKQVVSTWQPGESPSAAEAFWAFVAERVDGRNEVFSLEDEAAGDG
                     VQLHFFADSIARVTTVGPAADGQPEYRVEYALVDDLAHYRRMVRAYVTGGFAALADLG
                     HWYGDAAEFEQARKKGR"
     gene            complement(3316..3570)
                     /locus_tag="PLF56_09410"
     CDS             complement(3316..3570)
                     /locus_tag="PLF56_09410"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_013861544.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="type II toxin-antitoxin system VapB family
                     antitoxin"
                     /protein_id="HOQ53817.1"
                     /translation="MALNIKDPATDALARHLASATGESITEALRVAIAERLARVRRRA
                     ALGARSGELQRFIDRGRARPVLDARPADAILSYGDDGLPR"
     gene            complement(3698..>3957)
                     /locus_tag="PLF56_09415"
     CDS             complement(3698..>3957)
                     /locus_tag="PLF56_09415"
                     /inference="COORDINATES: protein motif:HMM:NF018745.2"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=3
                     /transl_table=11
                     /product="DUF1345 domain-containing protein"
                     /protein_id="HOQ53818.1"
                     /translation="IQWGFAQWYFAHYYSDVRPGMEFPGTEFPDLVDFAYFSFTIGTT
                     FATTDVMIVTRRARWRVVTHSVLTFFFNSAILVLALSSISS"
BASE COUNT          657 a         1314 c         1418 g          568 t
ORIGIN      
        1 cgaacccgtc atcccggcga acgccggaat cttccctgcg cactggtgca ccgttccgcc
       61 ggcccgcacg cagagatccc ggggcgagcc cgggatgacg gacacaccat tcgcacgagg
      121 tgcccccagg gcgagcccgg tatgacggga cgcacattcg cgcaacggcg ggacgccgat
      181 tcgtccaccc ccgatgacgc cccgtagcct gtcggggtgg ctttgacaat cggcatcgtc
      241 ggactgccca acgcgggcaa gtccaccctc ttcaacgcgc tcacccgcaa tgaggtgctg
      301 gcggcgaact atccgttcgc gaccatcgaa ccgaacgtcg gcgtggtcgg cgtgccggac
      361 gagcggctgc cgaaactcgc caaactgttc aagtctgcgc agatcattcc ggccacagtg
      421 agcttcgtcg acatcgccgg catcgtgaag ggtgccagcc agggcgaggg catgggcaac
      481 gcgttccttg ccaacattcg tgaggcggac gccatctgcc aggtcacccg ggtgttccgt
      541 gaccccgacg tcacccacgt cgacggggcg gtcaacccgg gcagcgacat cgacacgatc
      601 ggcaccgaac tgatcctggc cgacctgcag acgatcgagc gggcactccc ccggctcgag
      661 aaggaggccc ggctgcacaa ggaccgcaag gtggtgctcg aggccgtcca gcaggcgcag
      721 caggtgctcg actccgggcg cggcgtgcag gccgccggcc tcgacctgga gccgctcaag
      781 gaactgttcc tgctcaccgc gaagccctac atctacgtgt tcaactgcga ctccgacgaa
      841 ctcaccgacg agccactgct ggccggcatg cgcgaactgg tcgcccccgc cgaggcaatc
      901 ttcctcgacg ccaagatcga gtccgaactg gtcgaaatgg aaccggacga ggcccgggag
      961 ttcctcgagg agatgggcat cgacgagccg gggctggaca ccctggcccg ggtcggctac
     1021 gaaaccctcg gcctgcagag ctacctgacc gccggcccga aggagtcgcg agcctggacg
     1081 atccgcaagg gcgcgaccgc ccccgaggcg gccggggtga tccacaccga cttccagaag
     1141 gggttcatca aggccgagat cgtgtcgtac gacgacctcg tccgactggg cggcatgcag
     1201 caggccaagg cggccggcaa ggtgcgcctg gagggcaagg actacgtgat ggcggacggt
     1261 gacgtggtgg agttcagatt cagcacctga tccctgaagg cgcaactgca tcaggtcatg
     1321 cgggatgcga tgatatcgtg atatcagatt ggaggtgccg ctatgaccaa cgtgctgatt
     1381 cgtgggctca gcgaagccgc ggtcgaacgt atcgatgccg aggcggctgc gctggggctg
     1441 tcgcgcaacg agttcctgcg gcgccgtctc gagaaggaag cggtggcacc gcttgccgcc
     1501 acgatcaccg ctgacgactg gaggcggtcg gcggcggcct tcggtgacct taccgaccct
     1561 gacgtgatgg acgccgcgtg gcggtgacga gctggctggt cgacaagtcg gcctacgtgc
     1621 ggatgcaatc ggggcaggcg gcacagctgc aggagtggaa cggccggatc gagcgcgggc
     1681 tgatgcatct gtcggccgtg acgcggctcg agttggggtt ctccgcccgc tcgggcgcgg
     1741 agggtcgcga tgcgttcgga ctgccgccgc tgtccctcat gccgatcgaa catctgaccc
     1801 cggcgatcga ggatcgcgcc tgggagctgc agatgatgct ggctgatcgg gggcagcatc
     1861 gcgcaccgtc catcccggac ctactgatcg cagcgactgc ggagaagacc gggttgacgg
     1921 tgctcgccat cgacaaggac ttcgacctga tcgccgagat caccggccag ccggtgcaga
     1981 ctctggcggt gcggtgacca ccgtcgagcc cagccgggtg tccgatgagt gaggtgatct
     2041 tcgcccgcga cgaccagtgg ctgccgcggg tggtgcggga ggagggcggg ttgcgggccg
     2101 cggtggtggg cggcgccgat gccctgcacg gcccccgggt cttcagcttt ccgatcgagg
     2161 aagcgcacgt tgcggcgctg cgcgtcgacc tggcgcgaca cctgatcctc gacagcgcgc
     2221 tccggccgtt gtgcgatgac gccggcacca ggggcacgct ggacgagcac gcggccgtcg
     2281 cgctgctcga cccgatcctg ctggcgagcc cggacgacgt ggacgcgttc ctgcggcaca
     2341 ccaggtggga ccgcagcgcg ctggttgccc gcggcgccaa ccgggcgttg ctcgaccggg
     2401 gccgggtgat ggccgccctc ggcgccgccg acgggcgcgc tgactggagc cgcgcccagc
     2461 aggacacggc cgatcgcgac cgaagcaggc gcggcgtgac gctgtcagca ctcgacgcgg
     2521 cggtactccg cttcaccggc cagtacctgc atgctgcgac ggtgccgcgg cgcgatccgg
     2581 cagcggtcga tgccgagttg ctccctcagg tgcagcaggt gatcgccacg gccgagcagg
     2641 cctgcgcggg catcggcatc gcccgcgacc cccgccgcgg accgcgtgga acggacaagg
     2701 ccgactggcg ccggctggag tcgattgtgc agcaggcgtt gggtgccgcc cacccgcgac
     2761 tcggggccga tgcggtgggc agcgtcagct tcctgatctg cagcgaggcg gccggccgcg
     2821 ccctgctcgg cccctacgac accgacgact ccgctgcacc ggcaccgcga cgcacgctga
     2881 ccttcaccga cgacaagcag gtcgttagca cctggcagcc gggcgagagc ccgtccgccg
     2941 ccgaggcgtt ctgggcgttc gtcgccgaac gggtcgacgg ccgcaacgag gtgttcagcc
     3001 tcgaggacga ggcggccggc gacggcgtcc agttgcactt cttcgcggac tcgatcgccc
     3061 gggtcaccac ggtggggccg gctgccgatg ggcaaccgga gtaccgggtc gagtacgcac
     3121 tggtcgacga tctcgcccac taccgtcgga tggtgcgcgc ctacgtgacc ggaggcttcg
     3181 cggcactggc ggacctcggg cactggtacg gcgacgccgc ggagttcgag caggcgcgca
     3241 agaagggccg gtagcgccag gggtgacgcg ctccgcagcg tcgccggctg cgcgctcagg
     3301 cggaggtgtc cacgatcacc gcggcaggcc atcgtcgcca taggagagaa tcgcgtcggc
     3361 cgggcgggca tcgagaaccg gacgagcgcg gcctcggtcg atgaaccgct gcagttcgcc
     3421 gctgcgggca cccagtgcgg cacgtcgccg cacgcgggcc agccgctcgg cgatggcgac
     3481 gcgcaacgcc tcggtgatgc tctcgccggt ggcgctggcc agatggcgcg ccagggcgtc
     3541 ggtcgccggg tccttgatgt tcagtgccac gcgtccacac ttctgggaag aaaaagtgcc
     3601 cgacgaagaa gattcacgct gtggtaagcc gagagccgag ggggatggtg gtgccgggtg
     3661 aggtccttcc ccggacggcg ggagcgcgca gtggggttca gctgctgatg ctgctcaacg
     3721 ccagcaccag gatcgcgctg ttgaagaaga acgtcagcac cgagtgcgtc accacccgcc
     3781 agcgggcccg ccgggtgacg atcatcacgt ccgtggtggc gaaggtggta ccgatggtga
     3841 aggagaagta ggcgaagtcg accaggtcgg gaaactcggt gcccggaaac tccatgccgg
     3901 gccggacgtc gctgtagtag tgggcgaagt accactgggc gaacccccac tggatca
//
LOCUS       DAOSUG010000277         3957 bp    DNA     linear   ENV 10-JUN-2024
DEFINITION  MAG TPA_asm: Micropruina sp. isolate AMR_MDS_2195
            NODE_9520_length_3957_cov_3.151205, whole genome shotgun sequence.
ACCESSION   DAOSUG010000277 DAOSUG010000000
VERSION     DAOSUG010000277.1
DBLINK      BioProject: PRJNA925477
            BioSample: SAMN32864262
            Sequence Read Archive: SRR4236673
KEYWORDS    WGS; ENV; Metagenome Assembled Genome; MAG; Third Party Data; TPA;
            TPA:assembly.
SOURCE      Micropruina sp. (wastewater metagenome)
  ORGANISM  Micropruina sp.
            Bacteria; Bacillati; Actinomycetota; Actinomycetes;
            Propionibacteriales; Nocardioidaceae; Micropruina.
REFERENCE   1  (bases 1 to 3957)
  AUTHORS   Abdulkadir,N., Saraiva,J.P., Zhang,J., Stolte,S., Gillor,O.,
            Harms,H. and Rocha,U.
  TITLE     Genome-centric analyses of 165 metagenomes show that mobile genetic
            elements are crucial for the transmission of antimicrobial
            resistance genes to pathogens in activated sludge and wastewater
  JOURNAL   Microbiol Spectr 12 (3), e0291823 (2024)
   PUBMED   38289113
REFERENCE   2  (bases 1 to 3957)
  AUTHORS   Abdulkadir,N.
  TITLE     Direct Submission
  JOURNAL   Submitted (24-MAR-2023) Environmental Microbiology, Helmholtz
            Center for Environmental Research -UFZ, Permoserstr 15, Leipzig
            04318, Germany
COMMENT     The annotation was added by the NCBI Prokaryotic Genome Annotation
            Pipeline (PGAP). Information about PGAP can be found here:
            https://www.ncbi.nlm.nih.gov/genome/annotation_prok/
            
            ##Genome-Assembly-Data-START##
            Assembly Method        :: SPAdes v. 3.11.1
            Genome Representation  :: Full
            Expected Final Version :: Yes
            Genome Coverage        :: Not Applicable
            Sequencing Technology  :: Illumina MiSeq
            ##Genome-Assembly-Data-END##
            
            ##Genome-Annotation-Data-START##
            Annotation Provider               :: NCBI
            Annotation Date                   :: 05/04/2023 04:57:10
            Annotation Pipeline               :: NCBI Prokaryotic Genome
                                                 Annotation Pipeline (PGAP)
            Annotation Method                 :: Best-placed reference protein
                                                 set; GeneMarkS-2+
            Annotation Software revision      :: 6.5
            Features Annotated                :: Gene; CDS; rRNA; tRNA; ncRNA
            Genes (total)                     :: 2,776
            CDSs (total)                      :: 2,750
            Genes (coding)                    :: 2,714
            CDSs (with protein)               :: 2,714
            Genes (RNA)                       :: 26
            tRNAs                             :: 24
            ncRNAs                            :: 2
            Pseudo Genes (total)              :: 36
            CDSs (without protein)            :: 36
            Pseudo Genes (ambiguous residues) :: 0 of 36
            Pseudo Genes (frameshifted)       :: 2 of 36
            Pseudo Genes (incomplete)         :: 32 of 36
            Pseudo Genes (internal stop)      :: 2 of 36
            ##Genome-Annotation-Data-END##
FEATURES             Location/Qualifiers
     source          1..3957
                     /organism="Micropruina sp."
                     /mol_type="genomic DNA"
                     /submitter_seqid="NODE_9520_length_3957_cov_3.151205"
                     /isolate="AMR_MDS_2195"
                     /isolation_source="Activated sludge of municipal
                     wastewater treatment plants"
                     /db_xref="taxon:2737536"
                     /environmental_sample
                     /geo_loc_name="Canada"
                     /lat_lon="49.25 N 123.1 W"
                     /collection_date="2015-06-16"
                     /metagenome_source="wastewater metagenome"
                     /note="metagenomic"
     gene            401..1999
                     /gene="ffh"
                     /locus_tag="PLF56_09420"
     CDS             401..1999
                     /gene="ffh"
                     /locus_tag="PLF56_09420"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_002532496.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_component: GO:0048501 - signal recognition particle,
                     plasma membrane targeting [Evidence IEA];
                     GO_function: GO:0003924 - GTPase activity [Evidence IEA];
                     GO_function: GO:0005048 - signal sequence binding
                     [Evidence IEA];
                     GO_process: GO:0009306 - protein secretion [Evidence IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="signal recognition particle protein"
                     /protein_id="HOQ53819.1"
                     /translation="MFDTLQDRLSEVFKNLRGKSRLSEADIDATAREIRLALLEADVN
                     LGVVKEFIANLKSRALGAEVHKALNPSQQIIKLVNEELVAILGGEARPLRFAKRPPTV
                     IMLAGLQGSGKTTLAGKLGLWLKGQGHSPLLVAADLQRPNAVNQLQVVGERAGVHVYA
                     PEPGNGVGDPVQVARQSILHAMSKLYDVVVVDTAGRLGIDSELMQQAADIRAAVNPTE
                     VLFVVDAMIGQDAVNTASAFADGVGFDGVVLTKLDGDARGGAALSIAKVIGKPIMFAS
                     NGEGLKDFDAFHPDRMASRILGMGDVLTLIEQAEKTFDAEQTAKAAEKLLGGKGEFGL
                     DDFLQQMQAVRRMGPLSKILGMMPGMGQMKDQINNIDEREIDRLEAIIKSMTPAERAN
                     PKMLDGSRRTRIAKGSGTTVTDVNSLVNRFFEARKMMQSLGGQMGGPGMPGLPGGRRQ
                     PARQATKPKGRGKGVSGNPAKRAAGPEALPAAQLQPEWNPADAFGLGKAGQPSEAELA
                     KTLGDFQLPPDLQKMFNQKNQGPR"
     gene            2424..3620
                     /locus_tag="PLF56_09425"
     CDS             2424..3620
                     /locus_tag="PLF56_09425"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_015070824.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_function: GO:0016787 - hydrolase activity [Evidence
                     IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="amidohydrolase family protein"
                     /protein_id="HOQ53820.1"
                     /translation="MTHRHDLPPGLGPLTSLTHTHDGHTHTHVTSELPDGAPQRLHLR
                     GVILPEGETRDLWLHDGVISFEPVADAITVAGEGFIMPGLVDAHCHVGLESHGAVSEE
                     RAEEHALTDREVGALLLRDAGSPLDTRFLQDRDDLPKIIRAGRHIARPKRYSRNFAEE
                     VEPDQLVDEVVTQAERGDGWVKIVGDWIDRDAGDLTPLWPTDVAKEAIEAAHEHGARV
                     TAHCFDERSVAELVDAGIDGIEHGTGLEDDTIAAMAENGTSLVPTMVNLENFPMFAAA
                     GEAKFPRYAAHIMALYERRFETMGKAVDAGVPVYAGTDAGGVHGHGLIAGEIELLAKV
                     GGNDFALGAASWNARAWLGHPGIENGAPADLVVFADDPRVTISVVREPVLIILNGRVV
                     GGSAAH"
BASE COUNT          664 a         1323 c         1386 g          584 t
ORIGIN      
        1 ctccggctgg gcatcccagc gtcgcggacc gtcgggcggc atcggttggg acatggaggg
       61 cctccttcgc gtccggtcag tgtagagagg cctgggccga gtgactgctg ggggaagcgc
      121 tgatcatcgt cgcttaggga aacgctgatc aaggcccctc catcgtcatc tcggacttga
      181 tccgggatct ctcccgcctg aggtcccggg tcggagcccg ggatgacgcg aatgaccagc
      241 gcttccttag atgggcgtgt ggttcacaaa gggggtttcg acaagctcaa ccaacggtgg
      301 ggcgcatcga ctatcgaccg gatgagcaag acgggggcga atgtcgtcca cgatcgagcc
      361 ggtaggatgt ccgggatatc cgactgcgtg aggaagtccg ttgttcgaca ccctgcaaga
      421 ccgcctttcc gaggtcttca agaacctgcg cggcaagagc cggctgtccg aggccgacat
      481 cgacgccacc gcgcgcgaga tccggctggc gctgctcgag gccgacgtca acctgggcgt
      541 cgtcaaggag ttcatcgcca acctcaagtc gcgcgcgctg ggtgccgagg tgcacaaggc
      601 gctcaatccg agccagcaga tcatcaagct ggtcaacgag gaactggtcg cgatcctcgg
      661 tggcgaggcc cgcccgctgc gcttcgccaa gcgcccgccg acggtgatca tgctcgccgg
      721 tctgcagggc tccggtaaga cgaccctggc cggcaagctc ggcctgtggc tcaaggggca
      781 gggccattcg ccgctgctgg tggccgccga cctgcagcgg cccaacgccg tcaaccagtt
      841 gcaggtggtc ggcgagcgtg ccggcgtgca cgtgtacgcg cccgagccgg gcaacggcgt
      901 cggcgacccg gtgcaggtgg cgcggcagtc gattctgcac gccatgagca agctgtacga
      961 cgtggtcgtg gtcgacaccg ccggccggct gggcatcgac tccgaactca tgcagcaggc
     1021 cgccgacatt cgcgccgcgg tcaaccccac cgaggtgctg ttcgtggtcg acgcgatgat
     1081 cggccaggac gcggtgaaca ccgcgagtgc gttcgccgac ggcgtcgggt tcgacggcgt
     1141 ggtgctcacc aagctcgacg gcgacgcccg cggtggtgcc gcgctgtcga tcgccaaggt
     1201 gatcggcaag ccgatcatgt tcgcctccaa cggcgagggt ttgaaggact tcgacgcctt
     1261 ccaccccgac cggatggcca gccgcatcct gggcatgggc gatgtgctca ccctgatcga
     1321 acaggccgag aagaccttcg atgccgagca gaccgccaag gcggccgaga agctgctcgg
     1381 cggcaagggc gagttcggcc tggacgactt cctgcagcag atgcaggccg tccgacgcat
     1441 ggggccgctg tcgaagatcc tcggcatgat gcccggcatg ggtcagatga aggaccagat
     1501 caacaacatc gacgagcgcg agatcgaccg gctggaggcg atcatcaaat cgatgacccc
     1561 cgccgagcgg gccaacccca agatgctgga cgggtcgcgt cgcacccgca tcgccaaggg
     1621 ctcgggcacc acggtcaccg acgtgaacag cctggtcaac aggttcttcg aggcccgcaa
     1681 gatgatgcag tcgctgggcg gccagatggg tgggccgggc atgccggggt tgcccggtgg
     1741 tcgccgtcag ccggcacggc aggcgacgaa gccgaagggc cgcggcaagg gcgtctcggg
     1801 caatccggcc aagcgtgcag ccgggcccga ggcactaccc gccgcccagc tgcagcccga
     1861 gtggaaccct gcggacgcct tcggcctggg caaggcgggg cagccctccg aggccgagtt
     1921 ggcgaagacc ctcggcgatt ttcagctgcc gcccgacctg cagaagatgt tcaaccagaa
     1981 gaaccagggt ccgcgctagc aactcgctgg tcgagcttgt ccagaccctg ccggtgacgt
     2041 cgcccgcgtt gggtgacgtt gcccgcgttg caacgcgggc aacgtcacgg gcaacgtcag
     2101 ctatcgaggg caacgtcgcc aaggccaggc tggtcgggcc gttcaccagc cgccgctggt
     2161 cgagccgttc accagccggc gccggtcggg cttgtcgaga tctgcaccgc gcgagcgtgt
     2221 cgagaccacc tcatgggtcg agcttgccgt gacggtccga gcgccgactg ccgaaaagtg
     2281 cgcgccagcg aagccctcgg gtctcgacac gccgtacccg agcgcactgg cgcgcacctt
     2341 tcgtcgattc tcgttcggct atgccatgga acttcggggg aaccccacgc cccaaccttc
     2401 cggccggtgc catgatcgaa cccatgacgc accgccatga cctgccgccc ggcctgggac
     2461 cactgacatc gctcacccac acccacgacg ggcacacgca cacccacgtc acgtccgaac
     2521 tgccggacgg agcgccgcag cgcctgcacc tgcgcggcgt gattctgccc gagggtgaga
     2581 cgcgcgacct gtggctgcac gatggcgtga tcagcttcga gcccgtggcc gatgccatca
     2641 cggtcgccgg cgaggggttc atcatgcccg ggctggtgga cgcgcactgt cacgtcgggc
     2701 tcgaatcgca cggggcggtc agcgaggagc gggccgagga gcacgcgctg accgatcgcg
     2761 aggtgggcgc actgctgctg cgcgacgccg gatcgccgct cgacacccgc tttctgcagg
     2821 accgcgacga cctgccgaag atcatccgcg cgggccggca tatcgcccgt ccgaagcggt
     2881 actcgcgcaa cttcgccgaa gaggtcgagc ccgatcagct ggtcgacgag gtggtcaccc
     2941 aggccgagcg gggcgacggc tgggtcaaga tcgtcggcga ctggatcgac cgcgacgccg
     3001 gtgacctgac cccgctgtgg cccacggacg tggccaagga ggcgatcgaa gccgctcacg
     3061 aacacggtgc aagggtgacc gcgcactgct tcgacgagcg ctcggtggcc gaactcgtcg
     3121 acgccggcat cgacggcatc gagcacggca ccggactgga ggacgacacc atcgccgcga
     3181 tggccgagaa cggcacctcg ctggtgccga ccatggtgaa tctcgagaac ttcccgatgt
     3241 tcgcagcggc gggcgaggcc aagtttcccc gctacgcggc gcacatcatg gcgctgtacg
     3301 agcggcgctt cgagaccatg ggcaaggccg tggacgccgg cgttccggtt tacgcgggca
     3361 cggatgccgg gggagtgcac ggccacggcc tgatcgccgg cgagatcgaa ctgctggcca
     3421 aggtcggcgg caacgacttc gcgctcggcg cggcgtcgtg gaacgcgcgt gcctggctcg
     3481 gtcatcccgg catcgagaac ggcgccccgg ccgacctcgt ggtgttcgcc gacgacccgc
     3541 gggtgacgat ctcggtggtc cgcgagccgg tgctgatcat tctgaacgga cgggtggtgg
     3601 gcggctccgc ggcgcactga accgttcgcc ggaccacctg actgcagact cggcgtccgg
     3661 gcatgcgttg cgcacccgat cacgcgatgc cgctctcgtc gggtggcccg gacaccctgg
     3721 cgaacgtcca gcgtgcccat ttgggctgca actaggcaca actgggcagg gtctattggg
     3781 ccaggtccag gcgcagtcag gctggtgcgc cggcgagcag tcccgacggc gtccgaggtg
     3841 cgaccacgat gggcgtccgg gcgggtagcg tctggccgtg gatccgcacc ggctgagtgg
     3901 acgcgacgcg cgacggctgg ctgtgcgcgc ccaactgctg gacgcgcggc gtccggt
//
LOCUS       DAOSUG010000278         3951 bp    DNA     linear   ENV 10-JUN-2024
DEFINITION  MAG TPA_asm: Micropruina sp. isolate AMR_MDS_2195
            NODE_9536_length_3951_cov_5.353439, whole genome shotgun sequence.
ACCESSION   DAOSUG010000278 DAOSUG010000000
VERSION     DAOSUG010000278.1
DBLINK      BioProject: PRJNA925477
            BioSample: SAMN32864262
            Sequence Read Archive: SRR4236673
KEYWORDS    WGS; ENV; Metagenome Assembled Genome; MAG; Third Party Data; TPA;
            TPA:assembly.
SOURCE      Micropruina sp. (wastewater metagenome)
  ORGANISM  Micropruina sp.
            Bacteria; Bacillati; Actinomycetota; Actinomycetes;
            Propionibacteriales; Nocardioidaceae; Micropruina.
REFERENCE   1  (bases 1 to 3951)
  AUTHORS   Abdulkadir,N., Saraiva,J.P., Zhang,J., Stolte,S., Gillor,O.,
            Harms,H. and Rocha,U.
  TITLE     Genome-centric analyses of 165 metagenomes show that mobile genetic
            elements are crucial for the transmission of antimicrobial
            resistance genes to pathogens in activated sludge and wastewater
  JOURNAL   Microbiol Spectr 12 (3), e0291823 (2024)
   PUBMED   38289113
REFERENCE   2  (bases 1 to 3951)
  AUTHORS   Abdulkadir,N.
  TITLE     Direct Submission
  JOURNAL   Submitted (24-MAR-2023) Environmental Microbiology, Helmholtz
            Center for Environmental Research -UFZ, Permoserstr 15, Leipzig
            04318, Germany
COMMENT     The annotation was added by the NCBI Prokaryotic Genome Annotation
            Pipeline (PGAP). Information about PGAP can be found here:
            https://www.ncbi.nlm.nih.gov/genome/annotation_prok/
            
            ##Genome-Assembly-Data-START##
            Assembly Method        :: SPAdes v. 3.11.1
            Genome Representation  :: Full
            Expected Final Version :: Yes
            Genome Coverage        :: Not Applicable
            Sequencing Technology  :: Illumina MiSeq
            ##Genome-Assembly-Data-END##
            
            ##Genome-Annotation-Data-START##
            Annotation Provider               :: NCBI
            Annotation Date                   :: 05/04/2023 04:57:10
            Annotation Pipeline               :: NCBI Prokaryotic Genome
                                                 Annotation Pipeline (PGAP)
            Annotation Method                 :: Best-placed reference protein
                                                 set; GeneMarkS-2+
            Annotation Software revision      :: 6.5
            Features Annotated                :: Gene; CDS; rRNA; tRNA; ncRNA
            Genes (total)                     :: 2,776
            CDSs (total)                      :: 2,750
            Genes (coding)                    :: 2,714
            CDSs (with protein)               :: 2,714
            Genes (RNA)                       :: 26
            tRNAs                             :: 24
            ncRNAs                            :: 2
            Pseudo Genes (total)              :: 36
            CDSs (without protein)            :: 36
            Pseudo Genes (ambiguous residues) :: 0 of 36
            Pseudo Genes (frameshifted)       :: 2 of 36
            Pseudo Genes (incomplete)         :: 32 of 36
            Pseudo Genes (internal stop)      :: 2 of 36
            ##Genome-Annotation-Data-END##
FEATURES             Location/Qualifiers
     source          1..3951
                     /organism="Micropruina sp."
                     /mol_type="genomic DNA"
                     /submitter_seqid="NODE_9536_length_3951_cov_5.353439"
                     /isolate="AMR_MDS_2195"
                     /isolation_source="Activated sludge of municipal
                     wastewater treatment plants"
                     /db_xref="taxon:2737536"
                     /environmental_sample
                     /geo_loc_name="Canada"
                     /lat_lon="49.25 N 123.1 W"
                     /collection_date="2015-06-16"
                     /metagenome_source="wastewater metagenome"
                     /note="metagenomic"
     gene            complement(<1..585)
                     /locus_tag="PLF56_09430"
     CDS             complement(<1..585)
                     /locus_tag="PLF56_09430"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_013160163.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="beta-galactosidase"
                     /protein_id="HOQ53821.1"
                     /translation="MIVPRYYEDLAVLHENTLPARSYYIPSSTPLDPGPEARDSSDRL
                     QLLNGEWSFRYFESIHELRDHFYERDFPAGGFDRVPVPSTWQHQGYDHHQYTNIRYPF
                     PLDPPFVPQDNPCGAYVHEFEYTPSENAPSSHLTFEGVDSCFYVWLNGRYLGYSQISH
                     ATSEFDVTDLLQDGSNHLGVLVFKWCDGTYLEDQD"
     gene            complement(761..2083)
                     /locus_tag="PLF56_09435"
     CDS             complement(761..2083)
                     /locus_tag="PLF56_09435"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_076612250.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="ISL3-like element ISPfr3 family transposase"
                     /protein_id="HOQ53822.1"
                     /translation="MHDATVGGRADAFASPDLTAFCRLDELGLVVTGQRLEPDRAVLA
                     CQVVEPDQWCRRCGCEGRPRDTVVRRLAHEPLGWRPTTLEVVVRRYRCSGCGYVWRQD
                     TTAAAEPRAKLSRRALRWALEGIVVQHLTVARVAEGLGVAWDTANDAVLAEGKRVLID
                     EEHRFEGVKVVGVDEHVWRHTRRGDRYVTVIIDLTPVRDGTGPARLLDMVEGRSKQAF
                     KTWLADRPQEWRDGVEVVAMDGFTGFKTAAVEELPDVVTVLDPFHVTRLAGEALDECR
                     RRVQQAICGHRGRKGDPLYAARRTLSTGADLLNDKQKDRLDTLFADDAHVEVEVTWSV
                     YQRMIAAYRHENRRHGRELMARLIDSISTGVPKALVEITKLGRTLKKRAADVLAYFDR
                     PSTSNGPTEAINGRLEHLRGSALGFRNLTNYIARSLLETGGFRPRLHP"
     gene            2250..2864
                     /locus_tag="PLF56_09440"
     CDS             2250..2864
                     /locus_tag="PLF56_09440"
                     /inference="COORDINATES: ab initio
                     prediction:GeneMarkS-2+"
                     /note="Derived by automated computational analysis using
                     gene prediction method: GeneMarkS-2+."
                     /codon_start=1
                     /transl_table=11
                     /product="hypothetical protein"
                     /protein_id="HOQ53823.1"
                     /translation="MANHMSLLAQQAAQTPASVWPVLGGDFNTGQDDPLAARLLESMT
                     VVAGSGIDLIWVGKHETYKSSEPTTVPCGQLSDHDPTAITLEILNPPPPNLPLPPPSA
                     ELVVRFAPVPLVVPSGREGSVQVTVTALDAKTGEPKAGIVRVDPGSVGGTVTSSPTGH
                     RVSVPFRKTVRVDPVTKEHETTFVIARVTVSVAGYRDKAVDWDL"
     gene            3085..>3951
                     /locus_tag="PLF56_09445"
     CDS             3085..>3951
                     /locus_tag="PLF56_09445"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_011689697.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_function: GO:0004803 - transposase activity [Evidence
                     IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="IS256 family transposase"
                     /protein_id="HOQ53824.1"
                     /translation="MTDVANPRKAAPGSVAAQLIEQARAEGVSLVGPGSPLAALTKQV
                     LETALEAELDEHLGYEHGDRDTKAGAEQPNERNGTRVKTVHTELGPVSIEVPRDRHGS
                     FEPLVVRKRQRRLSGVDAMVLSLSAKGLTSGEICAFFADTYGQAMSKDTISRITDKVV
                     EEMTEWANRPLDSVYPVVFIDAIHVKVRDGQVTNKAFYVAIGVTVDGQRDILGIWAGA
                     GGEGAKHWLNVLTELKNRGVVDVLMLVCDGLKGLPEAVNTVWEHTTVQTCVIHLLRNT
                     FRYAARQHWDQIA"
BASE COUNT          699 a         1250 c         1332 g          670 t
ORIGIN      
        1 gtcctggtcc tctaggtagg tgccgtcgca ccacttgaag accagcacgc ccaggtggtt
       61 gctgccgtcc tggagcaggt cggtcacgtc gaactcgctg gtggcgtggg agatctggct
      121 gtagccgagg tagcggccgt tcagccacac gtagaagcag gagtcgacgc cttcgaaggt
      181 gagatgagag ctcggggcgt tttcactggg cgtgtactcg aactcgtgga cgtacgcgcc
      241 gcacggattg tcctgcggga cgaacggcgg atcgagcggg aaggggtagc ggatgttcgt
      301 gtactggtgg tggtcgtagc cctggtgctg ccacgtgctc ggcaccggca cacgatcgaa
      361 accgcccgcg gggaagtcgc gctcgtagaa gtggtcccga agctcgtgga tgctctcgaa
      421 gtaccggaac gaccactcac cgttcagcag ctgcagacga tcggaggagt cacgagcctc
      481 cggaccgggg tcgagcggcg tggaactcgg gatgtagtag gaacgtgccg ggagcgtgtt
      541 ctcgtgcaga acggccaagt cctcgtagta cctgggaacg atcatcgatg gatctcctct
      601 cggcccgctg tttttcacat gtgagtgggt gatgagccag ggtggtcggc aggctgcgcc
      661 acgatcggag ccgggcgagc gtgcccccac tcttcgcgca cctcgccgcg tccaagaccg
      721 cgccttcctt gcaggacgtt accggcaagg ctcatccgaa tcaagggtgt agtcggggtc
      781 tgaacccgcc ggtctcgagc agggatctgg cgatgtagtt ggtcaggttg cggaacccca
      841 gcgccgagcc gcgcaggtgc tcgagcctgc cgttgatcgc ctcggtgggc ccgttggagg
      901 tgctgggccg gtcgaagtag gccaggacgt cggcggcgcg cttcttcagc gtcctgccga
      961 gcttggtgat ctcgaccagg gccttgggga cgccggtgct gatcgagtcg atgagccggg
     1021 ccatcagctc gcggccgtgg cgccggttct cgtggcggta ggcggcgatc atgcgctggt
     1081 agacgctcca ggtgacctcg acctcgacgt gggcgtcgtc ggcgaacagg gtgtccagtc
     1141 ggtccttctg cttgtcgttg agcaggtcgg cgccggtgga cagggtccgg cgggcggcat
     1201 agagcgggtc gcccttgcgg ccgcggtgcc cacagatggc ctgctgaact cgccgtcggc
     1261 actcatcgag cgcctcgcca gcgaggcgcg tgacgtggaa gggatctagc acggtcacca
     1321 cgtcgggcag ttcctcgacg gcggcggtct tgaacccggt gaagccgtcc atcgcgacca
     1381 cctccacgcc gtcgcgccac tcctgcgggc ggtcggccag ccaggtcttg aacgcctgct
     1441 tggagcgtcc ttcgaccatg tccagcagcc gtgccgggcc ggtgccatct cgcactgggg
     1501 tgaggtcgat gatcacggtg acgtacctgt cgccgcgacg ggtgtgcctc cacacgtgct
     1561 catcgacccc gacgaccttc accccctcga agcggtgctc ctcgtcgatc agcacccgct
     1621 tgccctcggc cagaacggcg tcgttggcgg tgtcccaggc caccccgagc ccctcggcga
     1681 cccgagccac ggtcaggtgc tggaccacga tcccttccag cgcccaccgc agagcgcgcc
     1741 gagacaactt ggcccggggc tcggccgcgg cggtggtgtc ctggcgccac acatacccgc
     1801 agccgctgca gcggtaacgg cgcacgacga cctccagcgt cgtgggccgc cagcccagtg
     1861 gttcgtgggc aagccgtcgg accacggtgt cacgtggtct cccttcgcag ccgcagcgcc
     1921 ggcaccactg gtcgggctcg accacctggc aggccaatac ggcacgatcc ggctcgagtc
     1981 gctgcccggt gacaacgagg ccgagttcgt cgaggcggca gaaggcagtc aggtcggggc
     2041 tggcgaacgc atcagcgcgc ccgccgacgg tagcgtcgtg cacgtcgagg tctttcgggc
     2101 tgagtgtgta ggagctctca tcctcgggag acctcgacct ctatccggcc accgacgcgc
     2161 cggcccgccg cgcgacgtgc gctacaccct catctgggaa gagccgggta aggtccaccc
     2221 acgtcactca tcatgttcac agtgaataca tggccaacca catgagtctg ctcgctcagc
     2281 aggcggccca gaccccagca tcggtgtggc ctgtcctggg cggtgacttc aacacggggc
     2341 aggacgaccc tctggccgct cgattgcttg agtcgatgac cgtcgttgcc ggcagcggga
     2401 tcgacctgat ctgggtcggc aagcatgaga cgtacaagtc gagcgaaccc acaacggttc
     2461 catgcgggca gctatctgac cacgatccca cggctatcac cctggagata cttaacccac
     2521 ccccgccaaa cctgccactc ccgccaccct cggctgagct cgtggttcgc tttgcaccag
     2581 ttcccctggt cgttccttca ggcagggaag gctcggtgca ggtgactgta acggcgttag
     2641 acgccaagac tggggagccc aaggccggca ttgtccgagt cgaccccgga tctgtcggag
     2701 gaacggtgac atcatctccg accgggcaca gggtatccgt cccatttcga aagaccgttc
     2761 gggtcgaccc ggtgacaaaa gaacacgaaa ccacgttcgt gattgcccgc gtgaccgtct
     2821 cggtcgctgg ttaccgcgac aaggcggtcg actgggatct gtgaacctct tgaatcgaga
     2881 acaacccgaa tctggcgcac cagcgagtat gccagcccga caagataatg actaagcgac
     2941 accaacacga caagataatg agttatctag aggcgtgtgg ctaaaacggc tcttcacggg
     3001 actgtcagat aaacggtggg cgggtctggc ccgacacgcc gggtgaatgc ctggtgagga
     3061 aggcaggttc tcgtgaccga cacgatgacc gatgtggcaa atccgaggaa ggcggcgccg
     3121 ggttcggtag ctgcgcagct gatcgagcag gcccgggctg agggggtgtc gctggtcggg
     3181 cccggcagcc cgttggcggc gctgaccaag caggtcctgg aaaccgccct ggaggccgag
     3241 ctcgacgaac atctgggcta cgagcacggc gaccgggaca ccaaggccgg cgccgagcag
     3301 cccaacgagc gcaacggaac gagggtgaag acggttcaca ccgagctggg cccggtcagc
     3361 atcgaggtgc ccagggaccg gcacggctcg ttcgaacccc ttgtggtccg taagcggcag
     3421 cgccggctgt ccggggttga tgcgatggtg ttgtcgttgt cggcgaaagg actcacctct
     3481 ggtgagatct gtgcgttctt cgccgacacc tacggccaag cgatgagcaa ggacaccatc
     3541 tcccggatca ccgacaaagt ggtcgaggag atgaccgaat gggccaaccg gccactggac
     3601 tcggtgtacc cggtggtgtt catcgacgcg atccacgtca aggtccgcga cggacaggtc
     3661 accaacaagg cgttctacgt ggccatcggc gtcaccgtcg atgggcagcg agacattctg
     3721 gggatctggg ccggggccgg cggtgaaggc gccaaacact ggctgaacgt gctcaccgaa
     3781 ctgaagaacc gcggcgtcgt cgacgtgctc atgctcgtgt gtgacgggct gaagggtctg
     3841 ccggaggcgg tgaacaccgt gtgggagcac accaccgtcc aaacgtgtgt gatccacctg
     3901 ctgcgcaaca cgttccgcta cgccgcccgg cagcactggg accagatcgc c
//
LOCUS       DAOSUG010000279         3930 bp    DNA     linear   ENV 10-JUN-2024
DEFINITION  MAG TPA_asm: Micropruina sp. isolate AMR_MDS_2195
            NODE_9586_length_3930_cov_3.942194, whole genome shotgun sequence.
ACCESSION   DAOSUG010000279 DAOSUG010000000
VERSION     DAOSUG010000279.1
DBLINK      BioProject: PRJNA925477
            BioSample: SAMN32864262
            Sequence Read Archive: SRR4236673
KEYWORDS    WGS; ENV; Metagenome Assembled Genome; MAG; Third Party Data; TPA;
            TPA:assembly.
SOURCE      Micropruina sp. (wastewater metagenome)
  ORGANISM  Micropruina sp.
            Bacteria; Bacillati; Actinomycetota; Actinomycetes;
            Propionibacteriales; Nocardioidaceae; Micropruina.
REFERENCE   1  (bases 1 to 3930)
  AUTHORS   Abdulkadir,N., Saraiva,J.P., Zhang,J., Stolte,S., Gillor,O.,
            Harms,H. and Rocha,U.
  TITLE     Genome-centric analyses of 165 metagenomes show that mobile genetic
            elements are crucial for the transmission of antimicrobial
            resistance genes to pathogens in activated sludge and wastewater
  JOURNAL   Microbiol Spectr 12 (3), e0291823 (2024)
   PUBMED   38289113
REFERENCE   2  (bases 1 to 3930)
  AUTHORS   Abdulkadir,N.
  TITLE     Direct Submission
  JOURNAL   Submitted (24-MAR-2023) Environmental Microbiology, Helmholtz
            Center for Environmental Research -UFZ, Permoserstr 15, Leipzig
            04318, Germany
COMMENT     The annotation was added by the NCBI Prokaryotic Genome Annotation
            Pipeline (PGAP). Information about PGAP can be found here:
            https://www.ncbi.nlm.nih.gov/genome/annotation_prok/
            
            ##Genome-Assembly-Data-START##
            Assembly Method        :: SPAdes v. 3.11.1
            Genome Representation  :: Full
            Expected Final Version :: Yes
            Genome Coverage        :: Not Applicable
            Sequencing Technology  :: Illumina MiSeq
            ##Genome-Assembly-Data-END##
            
            ##Genome-Annotation-Data-START##
            Annotation Provider               :: NCBI
            Annotation Date                   :: 05/04/2023 04:57:10
            Annotation Pipeline               :: NCBI Prokaryotic Genome
                                                 Annotation Pipeline (PGAP)
            Annotation Method                 :: Best-placed reference protein
                                                 set; GeneMarkS-2+
            Annotation Software revision      :: 6.5
            Features Annotated                :: Gene; CDS; rRNA; tRNA; ncRNA
            Genes (total)                     :: 2,776
            CDSs (total)                      :: 2,750
            Genes (coding)                    :: 2,714
            CDSs (with protein)               :: 2,714
            Genes (RNA)                       :: 26
            tRNAs                             :: 24
            ncRNAs                            :: 2
            Pseudo Genes (total)              :: 36
            CDSs (without protein)            :: 36
            Pseudo Genes (ambiguous residues) :: 0 of 36
            Pseudo Genes (frameshifted)       :: 2 of 36
            Pseudo Genes (incomplete)         :: 32 of 36
            Pseudo Genes (internal stop)      :: 2 of 36
            ##Genome-Annotation-Data-END##
FEATURES             Location/Qualifiers
     source          1..3930
                     /organism="Micropruina sp."
                     /mol_type="genomic DNA"
                     /submitter_seqid="NODE_9586_length_3930_cov_3.942194"
                     /isolate="AMR_MDS_2195"
                     /isolation_source="Activated sludge of municipal
                     wastewater treatment plants"
                     /db_xref="taxon:2737536"
                     /environmental_sample
                     /geo_loc_name="Canada"
                     /lat_lon="49.25 N 123.1 W"
                     /collection_date="2015-06-16"
                     /metagenome_source="wastewater metagenome"
                     /note="metagenomic"
     gene            <1..579
                     /locus_tag="PLF56_09450"
     CDS             <1..579
                     /locus_tag="PLF56_09450"
                     /EC_number="3.1.3.-"
                     /inference="COORDINATES: protein motif:HMM:TIGR01549.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_function: GO:0016787 - hydrolase activity [Evidence
                     IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="HAD family hydrolase"
                     /protein_id="HOQ53825.1"
                     /translation="SGGQAFETQRALERRAMVDTLAALAVSVPSDQVADLLEPLFRYW
                     RTARPLPDALAFLRHWRLCPVVIVSNIDRADLEHLLPSLPAVAGLVTSQDARGYKPAP
                     SVFRHALALGGYAPQRVVHVGDSWESDVEGALLAGITPVWLNRTGAVAPASEVARIAS
                     FDELASCLNRLPDPDFTRPGAVAADPGEGVHG"
     gene            589..1575
                     /locus_tag="PLF56_09455"
     CDS             589..1575
                     /locus_tag="PLF56_09455"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_013861026.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="zinc-binding alcohol dehydrogenase"
                     /protein_id="HOQ53826.1"
                     /translation="MDATAYWTVAPGRGELRREQLATPGRDEVLVRTLASGISRGTEL
                     LVHRHGVPASVRERMRCPFQAGDLPGAVKYGYLNVGRVEAGPAHLLGERVFCLFPHQD
                     RFVVPVSAVTVVPVQVPTERAVLAGTVETAVNALWDGPPRIGDRVAVVGGGMVGLSVA
                     TLLRRFLLGRLQLVDVDPAKAHLARRLGVQHVQPDEAAADCDLVFHASASQAGLATAL
                     RLLGDEGELIELSWYGDTEPQVPLGADFHVRRLTIRASQVGRVSPTRAARRGPSDRLA
                     IALEQLRDPAFDALLAPAVAFDQLPQAMRELAAGRGTTLCQVINYPEEPSHV"
     gene            1568..1945
                     /locus_tag="PLF56_09460"
     CDS             1568..1945
                     /locus_tag="PLF56_09460"
                     /EC_number="4.1.2.50"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_017935920.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_function: GO:0046872 - metal ion binding [Evidence
                     IEA];
                     GO_function: GO:0070497 -
                     6-carboxy-5,6,7,8-tetrahydropterin synthase activity
                     [Evidence IEA];
                     GO_process: GO:0008616 - queuosine biosynthetic process
                     [Evidence IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="6-carboxytetrahydropterin synthase"
                     /protein_id="HOQ53827.1"
                     /translation="MYELSVRDHVMIAHSLPDPFFGPAAGLHGATFVVEVTWTRPDLD
                     EHGVVIDIGEASSRLAEVLGDLNYRNLDEHPAFAGRFSTTEAVAGHISERLLATMDLT
                     GFTALAVTLREHPDAWATCRVEL"
     gene            1961..2998
                     /locus_tag="PLF56_09465"
     CDS             1961..2998
                     /locus_tag="PLF56_09465"
                     /EC_number="2.4.-.-"
                     /inference="COORDINATES: protein motif:HMM:NF012744.2"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_function: GO:0016757 - glycosyltransferase activity
                     [Evidence IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="glycosyltransferase family 4 protein"
                     /protein_id="HOQ53828.1"
                     /translation="MPAPVFVMPRATAPSGGHAYNAAVLAHWPGEPPRVVELDGPWPR
                     GDSDSLRLLDQALGGSTLALVDGLVGAAHPEILGAAAVKDCRIVLLIHLPLADEGGLS
                     EADRAELGALERRSVRSAWRVVATSRTAARQLTHVTGRTDVIAVPPGVSPAPAAQAHQ
                     PPGIVQVGSLGPRKNQLASLQALTACRDLAFTATFAGPVADEAYAARVRAGLAAAVAT
                     WTGPLQGADLEGLYAGADLLLNPARAETWGMVITEALARGVPAIVGAGTGAVEALSSG
                     GAGGLPGAVVDLDDPDALARTVRRWLTDPALRQQWRDNALQARAGLRDWATSAAELAG
                     VLDPTNARSHE"
     gene            2995..3804
                     /locus_tag="PLF56_09470"
     CDS             2995..3804
                     /locus_tag="PLF56_09470"
                     /inference="COORDINATES: ab initio
                     prediction:GeneMarkS-2+"
                     /note="Derived by automated computational analysis using
                     gene prediction method: GeneMarkS-2+."
                     /codon_start=1
                     /transl_table=11
                     /product="hypothetical protein"
                     /protein_id="HOQ53829.1"
                     /translation="MTDSAGLRPERPIAADWLALRRSADTAARDRAAWLVRAATDGMT
                     GTLTVFDIGAGTGANQAYLTPRLGLPSRWVLLDHDADLLHAPGHGEATRVLGGIDELR
                     GLVAATSGSRLITCSALLDLLNTDDLDELASVMAQHRVRGLFSLTVDGSLAIEPSHRS
                     DTVIATAFNAHQARQGRPGPGAGGYLAQRCAQAGLVVRQAQTPWLLGAGAEALIERLL
                     TERGDAAVEQQPELAADAQQWLGDRRASLAVGSLRLQVGHVDLVVLPAEGR"
BASE COUNT          485 a         1380 c         1491 g          574 t
ORIGIN      
        1 agtggcgggc aggccttcga gacccagcgc gcgttggaac ggcgggcgat ggtcgacacc
       61 ctggctgcgc tggcggtgtc ggtgccctcc gatcaggtgg ccgacctgct cgagccgctg
      121 ttccggtact ggcgcaccgc tcgtccactg cccgatgcgc tcgcgtttct gcggcactgg
      181 cgcctgtgcc cggtcgtcat cgtgtcgaac atcgatcgcg cggacctgga gcacctgctg
      241 ccgagcctgc cggccgtggc cgggctggtg acgagccagg acgcgcgcgg gtacaagccc
      301 gccccgtctg tgtttcgcca cgccctggcc ctgggtggct atgcgccaca gcgcgtcgtc
      361 cacgtcggcg actcgtggga gtccgatgtc gaaggagcgc tgctggccgg catcaccccg
      421 gtctggctca atcgcaccgg tgccgtcgcg ccggcgtccg aggtggcgcg catcgcgtcg
      481 ttcgacgagt tggcgtcctg cctgaaccgg ctgccggacc cggatttcac ccgtccgggt
      541 gcggttgcgg cagacccggg cgagggcgtc cacgggtagc gtggcggaat ggatgcgacg
      601 gcgtactgga ccgttgcgcc gggtcgcggc gaactgcgcc gcgagcagct tgccaccccc
      661 ggccgcgacg aggtgctggt gcgtacgctc gcctcgggca tcagtcgcgg caccgaactg
      721 ctggtgcacc ggcacggggt gccagcctcg gttcgggagc ggatgcgatg cccgtttcag
      781 gccggtgacc tgcccggtgc ggtgaagtac ggctacctca acgtcggacg ggtcgaggcc
      841 ggtccggcgc acctgctcgg cgagcgggtc ttctgcctct tcccgcacca ggatcgcttc
      901 gtcgtaccgg tgagcgccgt cacggtggtg cccgtgcagg tgcccaccga acgcgccgtg
      961 ctggccggca ccgtcgaaac cgcggtcaac gccctgtggg acggcccgcc ccggatcggt
     1021 gaccgggtgg ccgtggtcgg cggcggcatg gtcgggctca gcgtggccac gctgctgcgc
     1081 cggtttctac tcggccggct tcaactggtc gacgtcgacc cggccaaggc gcacctggcg
     1141 cgcaggctcg gggtgcagca cgtccagccg gacgaggccg ccgccgactg cgacctggtg
     1201 ttccacgcct cggcgagcca ggccgggctg gccacggccc tgcgcctgct gggcgacgag
     1261 ggcgagttga tcgagctgtc ctggtacggc gacaccgagc cgcaggtccc gctcggcgcc
     1321 gactttcacg tgcgccggct gaccatccgg gccagccagg tgggccgggt cagcccgacg
     1381 cgggccgcgc ggcgcggacc gtccgaccgg ctggcgatcg cgctggaaca gctgcgcgac
     1441 ccagccttcg acgcactgct ggcgccggcc gttgccttcg accagttgcc gcaggcgatg
     1501 cgcgagttgg ccgccggccg cggcaccacg ctctgtcagg tgatcaacta tccggaggaa
     1561 ccgagccatg tatgagttga gcgtccgtga ccacgtgatg atcgcgcaca gcctgcccga
     1621 ccccttcttc ggccccgcgg ccggcctgca tggcgccacg ttcgtggtcg aggtgacctg
     1681 gacccggccc gacctggacg aacacggcgt ggtgatcgac atcggcgagg cctcgtcccg
     1741 gctggccgag gtgctgggcg acctgaacta ccgcaacctg gacgagcacc cggccttcgc
     1801 cggacggttc agcaccaccg aggcggtggc cgggcacatc agcgaacggc tgctggccac
     1861 gatggacctg accggtttca ccgccctggc cgtcacgctg cgggagcacc cggatgcctg
     1921 ggccacctgt cgggtcgagc tctagtggta cgggtctagg gtgcccgcgc ccgtcttcgt
     1981 gatgccgcgg gccaccgcgc cctcgggcgg ccacgcgtac aacgccgcgg tgctcgcgca
     2041 ctggccgggc gagccgccca gggtcgtcga gttggacggg ccgtggccgc gcggcgacag
     2101 cgactcgctt cggctgttgg accaggccct gggtggcagc acgctggcgc tggtggacgg
     2161 cctggtcggg gccgcccacc cggagattct cggggccgcc gccgtgaagg actgcaggat
     2221 cgtcctgctg atccacctgc cgctggcgga cgagggtggg ctgagcgagg ccgaccgggc
     2281 cgaactcggc gcgctggagc gccgcagcgt ccgctcggcc tggcgggtgg tcgcgaccag
     2341 tcgtacggcc gcccggcaac tgacacacgt gaccggccga accgacgtga tcgccgtccc
     2401 gccgggagtc agcccggcgc cagcggccca ggcacatcag ccgcccggca tcgtgcaggt
     2461 gggctccctc ggtccccgca agaaccagct cgcctcgctg caggcgctga ccgcatgccg
     2521 cgacctcgcc ttcaccgcga cgttcgcggg cccggtcgcc gacgaggcgt acgcggcccg
     2581 ggtccgggcg gggttggcgg cggcagtcgc cacctggacg ggaccgctgc agggtgccga
     2641 cctggagggg ctctacgcgg gtgccgacct gttgctgaac ccggcccggg ccgaaacctg
     2701 gggcatggtg atcaccgagg cgctcgcccg cggcgtcccg gcgatcgtcg gggcgggcac
     2761 cggagcggtg gaggcgctga gcagcggggg agccggcggg ctgccgggtg cggtggtcga
     2821 tctcgacgac ccggacgcgc tggcccgcac ggtgcgacgc tggctcaccg atccggcgct
     2881 gcggcagcag tggcgcgaca acgcgctgca ggcgcgggcc gggctgcgcg actgggcgac
     2941 gtcggccgcg gagctggccg gcgtgctcga ccctacgaac gcaaggagcc acgagtgacc
     3001 gattctgctg ggctgcgccc ggaacgtccg atcgccgcgg attggctggc actgcgccgc
     3061 agcgccgata cggcggcgcg cgaccgggcg gcatggttgg tgcgtgccgc gaccgacggc
     3121 atgacgggca cgctgacggt gttcgacatc ggtgccggca ccggcgccaa ccaggcctac
     3181 ctcaccccgc ggctcgggct gccgtcacgc tgggtgctgc tcgaccacga tgccgacctg
     3241 ctgcacgcgc cgggtcatgg tgaggcgacg cgggtgctgg gtggcatcga cgagctgcgc
     3301 gggctggtcg ccgcgacgtc cgggtcgcgg ctgatcacct gctcggccct gctcgacctg
     3361 ctgaacacgg acgacctgga cgaactggcg tccgtgatgg cacagcatcg ggtgcgcgga
     3421 ctgttcagcc tcaccgtcga cggctcgctc gcgatcgagc cctcgcatcg ttcggacacg
     3481 gtgatcgcca cggcgttcaa cgcgcatcag gcgcgtcagg gtcgtccggg gcccggcgcg
     3541 ggcggttatc tggcgcagcg ctgcgcgcag gccgggctgg tggtgcggca ggcacagacg
     3601 ccgtggctgc tgggcgccgg agccgaggcg ctgatcgagc gactgctcac cgaacgtggg
     3661 gacgctgcgg tggagcagca gcccgagctt gccgccgacg cgcagcagtg gcttggggat
     3721 cggcgggcca gcctggcggt ggggtcgctg cgcctgcagg tcggccatgt cgatctcgtg
     3781 gtcctgccgg ccgaggggcg ctgacggcgc tgacggccca ccgctggtcg cgcttggaga
     3841 gacctttgtg gtcgagtgtg ctctgttggg tcgggggcgg aggggggctc cactcgagcc
     3901 aaccagcctc ggtcgcaggc tccctcggcg
//
LOCUS       DAOSUG010000280         3929 bp    DNA     linear   ENV 10-JUN-2024
DEFINITION  MAG TPA_asm: Micropruina sp. isolate AMR_MDS_2195
            NODE_9589_length_3929_cov_4.006195, whole genome shotgun sequence.
ACCESSION   DAOSUG010000280 DAOSUG010000000
VERSION     DAOSUG010000280.1
DBLINK      BioProject: PRJNA925477
            BioSample: SAMN32864262
            Sequence Read Archive: SRR4236673
KEYWORDS    WGS; ENV; Metagenome Assembled Genome; MAG; Third Party Data; TPA;
            TPA:assembly.
SOURCE      Micropruina sp. (wastewater metagenome)
  ORGANISM  Micropruina sp.
            Bacteria; Bacillati; Actinomycetota; Actinomycetes;
            Propionibacteriales; Nocardioidaceae; Micropruina.
REFERENCE   1  (bases 1 to 3929)
  AUTHORS   Abdulkadir,N., Saraiva,J.P., Zhang,J., Stolte,S., Gillor,O.,
            Harms,H. and Rocha,U.
  TITLE     Genome-centric analyses of 165 metagenomes show that mobile genetic
            elements are crucial for the transmission of antimicrobial
            resistance genes to pathogens in activated sludge and wastewater
  JOURNAL   Microbiol Spectr 12 (3), e0291823 (2024)
   PUBMED   38289113
REFERENCE   2  (bases 1 to 3929)
  AUTHORS   Abdulkadir,N.
  TITLE     Direct Submission
  JOURNAL   Submitted (24-MAR-2023) Environmental Microbiology, Helmholtz
            Center for Environmental Research -UFZ, Permoserstr 15, Leipzig
            04318, Germany
COMMENT     The annotation was added by the NCBI Prokaryotic Genome Annotation
            Pipeline (PGAP). Information about PGAP can be found here:
            https://www.ncbi.nlm.nih.gov/genome/annotation_prok/
            
            ##Genome-Assembly-Data-START##
            Assembly Method        :: SPAdes v. 3.11.1
            Genome Representation  :: Full
            Expected Final Version :: Yes
            Genome Coverage        :: Not Applicable
            Sequencing Technology  :: Illumina MiSeq
            ##Genome-Assembly-Data-END##
            
            ##Genome-Annotation-Data-START##
            Annotation Provider               :: NCBI
            Annotation Date                   :: 05/04/2023 04:57:10
            Annotation Pipeline               :: NCBI Prokaryotic Genome
                                                 Annotation Pipeline (PGAP)
            Annotation Method                 :: Best-placed reference protein
                                                 set; GeneMarkS-2+
            Annotation Software revision      :: 6.5
            Features Annotated                :: Gene; CDS; rRNA; tRNA; ncRNA
            Genes (total)                     :: 2,776
            CDSs (total)                      :: 2,750
            Genes (coding)                    :: 2,714
            CDSs (with protein)               :: 2,714
            Genes (RNA)                       :: 26
            tRNAs                             :: 24
            ncRNAs                            :: 2
            Pseudo Genes (total)              :: 36
            CDSs (without protein)            :: 36
            Pseudo Genes (ambiguous residues) :: 0 of 36
            Pseudo Genes (frameshifted)       :: 2 of 36
            Pseudo Genes (incomplete)         :: 32 of 36
            Pseudo Genes (internal stop)      :: 2 of 36
            ##Genome-Annotation-Data-END##
FEATURES             Location/Qualifiers
     source          1..3929
                     /organism="Micropruina sp."
                     /mol_type="genomic DNA"
                     /submitter_seqid="NODE_9589_length_3929_cov_4.006195"
                     /isolate="AMR_MDS_2195"
                     /isolation_source="Activated sludge of municipal
                     wastewater treatment plants"
                     /db_xref="taxon:2737536"
                     /environmental_sample
                     /geo_loc_name="Canada"
                     /lat_lon="49.25 N 123.1 W"
                     /collection_date="2015-06-16"
                     /metagenome_source="wastewater metagenome"
                     /note="metagenomic"
     gene            complement(<1..441)
                     /locus_tag="PLF56_09475"
     CDS             complement(<1..441)
                     /locus_tag="PLF56_09475"
                     /inference="COORDINATES: protein motif:HMM:NF013694.2"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_component: GO:0016020 - membrane [Evidence IEA];
                     GO_process: GO:0055085 - transmembrane transport [Evidence
                     IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="CorA family divalent cation transporter"
                     /protein_id="HOQ53830.1"
                     /translation="MTQLTSRVWRDGELVASDIPLSELDGYLADAGALTWLDLCDPEG
                     EVLSQLAEELRLDRHAVEDSIAPRERAKLSRHATHLFLTAYSASLGVDSQGLPELQVG
                     RISAFVMPRGLITVRLMRGAVIEPDELVARWTDAGLLAQGVSALV"
     gene            complement(449..1351)
                     /locus_tag="PLF56_09480"
     CDS             complement(449..1351)
                     /locus_tag="PLF56_09480"
                     /inference="COORDINATES: protein motif:HMM:NF015431.2"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="LysR substrate-binding domain-containing
                     protein"
                     /protein_id="HOQ53831.1"
                     /translation="METRRLATFVRIVDVGSLTRAADVLHIAQPALSQQINALEAELG
                     QRLLTRSKQGVEPTEAGAALYRHAQVILKQLDDAVAEVGLLGREVAGQVYVGLAPYST
                     ANLIALPLVRAVRERYPHVMLRVSDNFGLVLSEAMMTGRLHLAILYDSGPIKGLAFER
                     LISEELVLVSCPDKPPVADEVAVETLAEVPLLLPSPLHTIRKVVDRACETAGVQPLLV
                     GELESVGLMGQAVAAGLGATLLPRSIADKLAADSGLTTTRLTPGLDVHLSIGRPSSLP
                     LSRAAECVRDLMRHVVAATLLGRA"
     gene            1464..2243
                     /locus_tag="PLF56_09485"
     CDS             1464..2243
                     /locus_tag="PLF56_09485"
                     /EC_number="3.5.2.9"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_013862873.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_function: GO:0005524 - ATP binding [Evidence IEA];
                     GO_function: GO:0016810 - hydrolase activity, acting on
                     carbon-nitrogen (but not peptide) bonds [Evidence IEA];
                     GO_function: GO:0017168 - 5-oxoprolinase (ATP-hydrolyzing)
                     activity [Evidence IEA];
                     GO_process: GO:0005975 - carbohydrate metabolic process
                     [Evidence IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="5-oxoprolinase subunit PxpA"
                     /protein_id="HOQ53832.1"
                     /translation="MQSTIDLVADLGEGFGDWTMGDDPALLEIVSSANVACGYHAGDP
                     RIMDATARACAERGIQIGAHPSFPDLVGFGRRDMGLSAEQVRTDTMYQIGALSAFAAA
                     HGTRVRHVAPHGRLGNLVAVRADYAKAVCDAACAVDPDLIVVCQDGELADEARRRGLR
                     TAIVGIVDRAYRPDGTLVPRSEPGAVIHDLDVLVARAIRIATESTLEAVDGSVLSVHA
                     DSLLLHGDNPGAVATARAIRAGLTRAGVTVAPMADVLASRG"
     gene            2281..2955
                     /locus_tag="PLF56_09490"
     CDS             2281..2955
                     /locus_tag="PLF56_09490"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_013862872.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="carboxyltransferase domain-containing protein"
                     /protein_id="HOQ53833.1"
                     /translation="MLDCGDSALRVVVEGPAASSAWRIVHGLAALLRQAAPQGLLGTL
                     PTYDSLLVEFDCARTDHATLRAAIGQLADRIDGVPARTPRTFDVPVVYGGEHGPDLDA
                     VAAHLDLSPDEVIARHTAEPLLIRCFGSPAGAPMMDGPAFGRPVPRLASPRPHVPAGA
                     VAVAGHQAVVSACPAPGGWHVLGTTPLRMVVPDREPLAPYRPGDSLRFHAIDASDFEA
                     CAGVLS"
     gene            2952..>3929
                     /locus_tag="PLF56_09495"
     CDS             2952..>3929
                     /locus_tag="PLF56_09495"
                     /inference="COORDINATES: protein motif:HMM:NF014663.2"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="biotin-dependent carboxyltransferase family
                     protein"
                     /protein_id="HOQ53834.1"
                     /translation="MNGRVTITRPGISAVTDLGRFAGGAVGQMTGGALDQYSARVANA
                     LVGADQGAPLVELVAADFAARTSANLLIAVTGAPADVTVNGIPMPQWEPFWWPAGTEL
                     AIGSIRLGMRVYVAVHGSLDAPLLLGSCAPDSVLGFSGHLHRGDTIDVEPGPVRIYHS
                     PLGLPVMRAAVSQPGFSDHWRIPVTDGPDRAQFGAGAERLHAGRFVIGTNSNHVGLRL
                     SPEPGASVPVRERTDEVLSRGVPIGAVEVPAGSELLVLHRGRGVTAGYPVLAVVTRVG
                     LSLLGQARPGQSVEFVRVTLEEAIAAARAEQAAIDGLTWRVARLVSAHGA"
BASE COUNT          527 a         1445 c         1367 g          590 t
ORIGIN      
        1 cacgagcgca ctgaccccct gggcgagcag gcccgcatcc gtccagcggg cgacgagttc
       61 gtccggctcg atcacggccc cgcgcatcag ccgcacggtg atcagccccc gcggcatcac
      121 gaacgccgag atgcggccca cctgcaactc gggcaggccc tgcgagtcga cgccgagact
      181 cgccgagtag gcggtcagga acaggtgggt ggcgtgccgg ctgagcttgg ctcgttcgcg
      241 cggcgcgatc gagtcctcga ccgcgtgccg gtcgagccgc agctcctcgg cgagctggct
      301 caacacctcg ccctccgggt cgcacaggtc gagccaggtc agtgcgccgg cgtcggccag
      361 atacccgtcc agttcgctca gcggaatatc ggacgccacc aactcgccat cgcgccagac
      421 ccggctggtc agctgcgtca tcggcggttc acgcccggcc cagcagggtc gccgccacga
      481 cgtgtcgcat caggtcgcgg acgcactcgg ccgcgcgcga cagcggcaag ctggacggtc
      541 tgccgatcga caggtgcaca tcgaggccgg gcgtgaggcg ggtcgtggtc agccccgagt
      601 cggcggcaag cttgtccgcg atggagcgcg gcagcagggt ggcacccaat ccggcggcga
      661 ccgcctgccc catcagcccc accgactcca actcacccac cagcaacggt tggacgccag
      721 ccgtctcgca cgcccggtcg accaccttgc gaatcgtgtg cagggggctg ggcagcagca
      781 gcggcacctc ggcgagcgtc tcgaccgcca cctcgtccgc cacgggtggc ttgtcggggc
      841 acgacaccag caccagctcc tcactgatca gccgttcgaa cgccagcccc ttgatcggtc
      901 ccgagtcgta caggatcgcc agatgcagcc ggccggtcat catggcctcg ctgagcacca
      961 ggccgaaatt gtcgctgacc cgcagcatca cgtgcgggta ccgctcgcgc accgcccgca
     1021 ccagcggcag cgcgatcagg ttggccgtgc tgtagggggc cagtccgacg tacacctgac
     1081 cggcgacctc gcgtccgagc aggcccacct cggccacggc gtcgtccaac tgcttgagaa
     1141 tcacctgcgc gtgccggtag agcgcggccc cggcttcggt cggctcgacg ccctgcttgc
     1201 tgcgggtcag cagccgctgg ccgagttcgg cttccagcgc gttgatctgc tggctgagtg
     1261 cgggctgcgc gatgtgcagc acgtcggcgg cgcgggtcag gctgcccacg tcgacgattc
     1321 ggacgaaggt cgccaagcgt ctggtctcca tggcacctcc ggtgcagcca tcgtgcggcg
     1381 ctatcacatc agcgaaatca tgtcttacca caatgcgtcg gatattcctt acggtcagtt
     1441 cattcagtca cgaaggagca gcgatgcagt caacgatcga tctggtggcc gacctcggtg
     1501 agggtttcgg cgactggacg atgggcgacg atccggcgct gctcgagatc gtcagctcgg
     1561 ccaacgtggc ctgcggctat cacgccggcg atccccgcat catggacgcc accgcaaggg
     1621 cctgcgccga acgcggcatt cagatcggcg cccatcccag ctttcccgac ctggtcgggt
     1681 tcgggcggcg cgacatgggg ctgagcgccg agcaggtacg caccgacacg atgtaccaga
     1741 tcggcgcact ctcggccttc gccgccgcgc acggcacccg ggtgcgccac gtcgccccgc
     1801 acggacgcct gggtaacctg gtggccgtgc gcgccgacta tgcgaaggcc gtgtgcgacg
     1861 cggcctgcgc cgtcgacccc gacctgatcg tggtctgcca ggacggcgag ctggccgatg
     1921 aggcccgccg gcgcggcctg cgcacggcga tcgtcggcat cgtcgatcgc gcgtaccgtc
     1981 cggacggcac gttggtgccg cgctccgagc cgggcgcggt gatccacgac ctggacgtgc
     2041 tggtggcccg ggcgatccgg atcgccaccg aatcgacgct cgaggccgtg gacgggtcgg
     2101 tgctcagcgt gcacgccgac tcgctgctgc tgcacggcga caatcccggc gccgtggcca
     2161 ctgcccgggc gatccgggcc ggactcaccc gagcgggcgt caccgtggcc ccgatggccg
     2221 acgtgctcgc gagccggggc tgacatgacg aacctgcggt cgggcggttg cgccgttgac
     2281 atgctcgatt gtggggattc ggcgctgcgg gtcgtcgtgg agggccccgc cgcgtcgtcc
     2341 gcgtggcgga tcgttcacgg gctggccgcc ctgctgcgcc aggcagcacc gcagggcctg
     2401 ctgggcaccc tgcccaccta cgattcgctg ctggtcgagt tcgactgcgc ccgcaccgac
     2461 cacgccacgc tgcgggccgc gatcggtcag ctcgccgacc ggatcgacgg cgtgccggcc
     2521 cgcacgccac gcaccttcga cgttccggtc gtctacggcg gcgagcacgg gccggacctg
     2581 gacgcggtcg ccgcgcacct cgacctgagc cccgacgagg tgatcgcccg gcacaccgcc
     2641 gagccgctgc tgatccgttg tttcggttcg cccgccggcg ccccgatgat ggacggcccg
     2701 gcattcggac gtccggtgcc ccggctcgcc agcccccgtc cgcacgtgcc cgccggcgcg
     2761 gtcgcggtgg ccggccatca ggcggtcgtc tcggcctgtc cggcaccggg aggctggcac
     2821 gtgctgggca ccacgccgct gcgcatggtg gtgcccgatc gcgaaccact cgccccctac
     2881 cggccgggcg actcgctgcg gttccatgcc atcgacgcgt ccgatttcga ggcctgtgcg
     2941 ggggtgctct cgtgaacggt cgcgtcacca tcacccgccc cggcatcagt gccgtgaccg
     3001 atctgggccg cttcgccggc ggcgcggtgg gtcagatgac cgggggggcc ctcgaccagt
     3061 acagcgcccg cgttgccaac gctctggtcg gcgccgatca gggggccccg ctggtcgagc
     3121 tggtggcggc cgacttcgcc gctcgcacca gcgccaacct gttgatcgcc gtcaccggcg
     3181 ccccggccga tgtcaccgtc aacggcattc cgatgccgca gtgggaaccc ttctggtggc
     3241 cggccggcac cgagctcgcc atcggttcga tccggctcgg catgcgcgtc tacgtggctg
     3301 tgcacggcag cctggacgct ccgctgctgc tcggcagctg tgctcccgac agcgtgctgg
     3361 gtttctcggg gcacctgcat cgcggcgaca cgatcgacgt cgaaccgggg ccggtgcgga
     3421 tctatcattc gcccctcggc cttccggtga tgcgcgccgc ggtgtcgcag cccgggttca
     3481 gcgatcattg gcgcattccg gtcaccgacg gtcccgaccg ggcccagttc ggcgccggcg
     3541 ccgagcggct gcacgccggc cgtttcgtga tcggcaccaa cagcaaccac gtcgggctgc
     3601 ggctctcacc cgaaccgggg gcgtccgtgc cggtgcgtga acgcaccgac gaggtgctca
     3661 gccggggcgt cccgatcggc gcggtggagg tgccggccgg cagcgagctg ctggtgctgc
     3721 accgcggtcg tggggtcacc gcgggctacc cggtgctcgc cgtggtgacc cgcgtcggct
     3781 tgtccctgct cggccaggcc cgacccggcc agagcgtcga gttcgtccgg gtcacgctcg
     3841 aggaggcgat cgcggccgca cgcgccgagc aggccgccat cgacggcctc acctggcggg
     3901 tcgcccggtt ggtgtcggcg cacggggcc
//
LOCUS       DAOSUG010000281         3928 bp    DNA     linear   ENV 10-JUN-2024
DEFINITION  MAG TPA_asm: Micropruina sp. isolate AMR_MDS_2195
            NODE_9595_length_3928_cov_4.310096, whole genome shotgun sequence.
ACCESSION   DAOSUG010000281 DAOSUG010000000
VERSION     DAOSUG010000281.1
DBLINK      BioProject: PRJNA925477
            BioSample: SAMN32864262
            Sequence Read Archive: SRR4236673
KEYWORDS    WGS; ENV; Metagenome Assembled Genome; MAG; Third Party Data; TPA;
            TPA:assembly.
SOURCE      Micropruina sp. (wastewater metagenome)
  ORGANISM  Micropruina sp.
            Bacteria; Bacillati; Actinomycetota; Actinomycetes;
            Propionibacteriales; Nocardioidaceae; Micropruina.
REFERENCE   1  (bases 1 to 3928)
  AUTHORS   Abdulkadir,N., Saraiva,J.P., Zhang,J., Stolte,S., Gillor,O.,
            Harms,H. and Rocha,U.
  TITLE     Genome-centric analyses of 165 metagenomes show that mobile genetic
            elements are crucial for the transmission of antimicrobial
            resistance genes to pathogens in activated sludge and wastewater
  JOURNAL   Microbiol Spectr 12 (3), e0291823 (2024)
   PUBMED   38289113
REFERENCE   2  (bases 1 to 3928)
  AUTHORS   Abdulkadir,N.
  TITLE     Direct Submission
  JOURNAL   Submitted (24-MAR-2023) Environmental Microbiology, Helmholtz
            Center for Environmental Research -UFZ, Permoserstr 15, Leipzig
            04318, Germany
COMMENT     The annotation was added by the NCBI Prokaryotic Genome Annotation
            Pipeline (PGAP). Information about PGAP can be found here:
            https://www.ncbi.nlm.nih.gov/genome/annotation_prok/
            
            ##Genome-Assembly-Data-START##
            Assembly Method        :: SPAdes v. 3.11.1
            Genome Representation  :: Full
            Expected Final Version :: Yes
            Genome Coverage        :: Not Applicable
            Sequencing Technology  :: Illumina MiSeq
            ##Genome-Assembly-Data-END##
            
            ##Genome-Annotation-Data-START##
            Annotation Provider               :: NCBI
            Annotation Date                   :: 05/04/2023 04:57:10
            Annotation Pipeline               :: NCBI Prokaryotic Genome
                                                 Annotation Pipeline (PGAP)
            Annotation Method                 :: Best-placed reference protein
                                                 set; GeneMarkS-2+
            Annotation Software revision      :: 6.5
            Features Annotated                :: Gene; CDS; rRNA; tRNA; ncRNA
            Genes (total)                     :: 2,776
            CDSs (total)                      :: 2,750
            Genes (coding)                    :: 2,714
            CDSs (with protein)               :: 2,714
            Genes (RNA)                       :: 26
            tRNAs                             :: 24
            ncRNAs                            :: 2
            Pseudo Genes (total)              :: 36
            CDSs (without protein)            :: 36
            Pseudo Genes (ambiguous residues) :: 0 of 36
            Pseudo Genes (frameshifted)       :: 2 of 36
            Pseudo Genes (incomplete)         :: 32 of 36
            Pseudo Genes (internal stop)      :: 2 of 36
            ##Genome-Annotation-Data-END##
FEATURES             Location/Qualifiers
     source          1..3928
                     /organism="Micropruina sp."
                     /mol_type="genomic DNA"
                     /submitter_seqid="NODE_9595_length_3928_cov_4.310096"
                     /isolate="AMR_MDS_2195"
                     /isolation_source="Activated sludge of municipal
                     wastewater treatment plants"
                     /db_xref="taxon:2737536"
                     /environmental_sample
                     /geo_loc_name="Canada"
                     /lat_lon="49.25 N 123.1 W"
                     /collection_date="2015-06-16"
                     /metagenome_source="wastewater metagenome"
                     /note="metagenomic"
     gene            <1..481
                     /locus_tag="PLF56_09500"
     CDS             <1..481
                     /locus_tag="PLF56_09500"
                     /inference="COORDINATES: ab initio
                     prediction:GeneMarkS-2+"
                     /note="Derived by automated computational analysis using
                     gene prediction method: GeneMarkS-2+."
                     /codon_start=2
                     /transl_table=11
                     /product="hypothetical protein"
                     /protein_id="HOQ53835.1"
                     /translation="VSRLRDGAPESAAAPALSAAGFVIDRAIEPLRAPVLVRLDSPAN
                     IAAGDTVWGLGDHLRHRLRAALPGCTLLTVQTPDELTAALAAADGRPLVVQGRDMARV
                     PFLSECATTVRRHRPDALLVELGWPDPATSTPPDLVTFGSGAGQMSCLIDLLERGTR"
     gene            478..1470
                     /locus_tag="PLF56_09505"
     CDS             478..1470
                     /locus_tag="PLF56_09505"
                     /inference="COORDINATES: protein motif:HMM:NF012692.2"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="ROK family protein"
                     /protein_id="HOQ53836.1"
                     /translation="MSPGTEQPTTLGIDIGGTKTQGIVLDGQARVIADRRRRTATGPA
                     GVLATALAVARDCLQLAGVRQEHLGSVGVGIPGRVDHRTGVVSTAVNLGIGRLELGSA
                     LSAELGVPVQVDNDVKATALGAADYLRAGDADLTYLNLGTGVASATLIGGRLVRGDDN
                     LAGEIGHVPIDPGAGRCACGQRGCIEVLAGGAQIAGRLAALGSGLTLRTLVSAAEAGD
                     PAADDEVRRIAEAIATAVQLLVLAHGSTRVALGGGVVHTAPGLVEQSRRALDRRAEHS
                     PFLRSLELSARITLVPAAHPLAAIGAALVGRAGLARAHWERPARMGAAPRERQP"
     gene            1467..2156
                     /locus_tag="PLF56_09510"
     CDS             1467..2156
                     /locus_tag="PLF56_09510"
                     /inference="COORDINATES: ab initio
                     prediction:GeneMarkS-2+"
                     /note="Derived by automated computational analysis using
                     gene prediction method: GeneMarkS-2+."
                     /codon_start=1
                     /transl_table=11
                     /product="hypothetical protein"
                     /protein_id="HOQ53837.1"
                     /translation="MTQGNTPQYPQQHDPYRPGYGQPGQPDQSEWGQPGTQAGYGAGG
                     QQPAGYPQQSYPQQSYPQQGYSQDGYQQAYPQQSYPQQGYQAPGYPAAYPQTMPYGQA
                     GPYAQAEQPRSPLLGMISLGVVVICTVIFGWVMFRMGALLAPVLVTNAGAATQQQMTQ
                     ELLDRLGATGLLLLNVSTYGGFAAWITGIVATATKRGRSYGIWAIIIGALAPFIGFGL
                     MLAALMPYINA"
     gene            complement(2168..3328)
                     /gene="dusB"
                     /locus_tag="PLF56_09515"
     CDS             complement(2168..3328)
                     /gene="dusB"
                     /locus_tag="PLF56_09515"
                     /EC_number="1.3.1.-"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_013864245.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_function: GO:0003824 - catalytic activity [Evidence
                     IEA];
                     GO_function: GO:0016491 - oxidoreductase activity
                     [Evidence IEA];
                     GO_function: GO:0017150 - tRNA dihydrouridine synthase
                     activity [Evidence IEA];
                     GO_function: GO:0050660 - flavin adenine dinucleotide
                     binding [Evidence IEA];
                     GO_process: GO:0008033 - tRNA processing [Evidence IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="tRNA dihydrouridine synthase DusB"
                     /protein_id="HOQ53838.1"
                     /translation="MRSLQTETAPQTETLRLGPITVDPPVVLAPMAGITNAAYRQLCR
                     EQGAGLYVCEMITSRAVVERIPKTFAMLRFDPGETVRSVQLYGVDPAVMAKATTILCD
                     EFGVSHVDLNFGCPVPKVTRRGGGGVLPWKRDRLAAILTATVRAAEPYGVPVTMKTRL
                     GIDEQHETFLDAGRIAREAGCAAIALHARTVKQAYGGEADWSRIAELVASVDLPVLGN
                     GDIWEAADALAMMRRTGCAGVVIGRGCLGRPWLFRDLAAAFAGREAPPLPNLGEVAAM
                     VYRHAELLAGLDSETHGLRDLRRHMAWYFKGFAVGGDVRRGLATVESLAQLRGLLATL
                     DADVPYPSSELGSPRGRQGAPRGKVVLPYGWLDSDCLGEADIADAESDASGG"
     gene            complement(3365..>3928)
                     /locus_tag="PLF56_09520"
     CDS             complement(3365..>3928)
                     /locus_tag="PLF56_09520"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_012924333.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_function: GO:0003677 - DNA binding [Evidence IEA];
                     GO_process: GO:0000160 - phosphorelay signal transduction
                     system [Evidence IEA];
                     GO_process: GO:0006355 - regulation of DNA-templated
                     transcription [Evidence IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="winged helix-turn-helix domain-containing
                     protein"
                     /protein_id="HOQ53839.1"
                     /translation="AAAAHPPELVLVDLGLPDLDGVEVIAGLRGWSRVPIIVLSARDT
                     QQQKVRALDAGADDYVTKPFGMDELLARVRAALRRADVPEPSELPVIGTDSFTLDLAA
                     KRALRHGAEVKLTPTEWHLVEALVRRPGTLVPGAQLLAEVWGPGYENQGNYLRVYFAQ
                     LRRKLEDAPGEPRHFITEPGLGYRFDP"
BASE COUNT          554 a         1500 c         1357 g          517 t
ORIGIN      
        1 cgtgtcaagg ctgcgcgacg gtgcgccgga atccgccgca gcgcccgcgc tcagcgccgc
       61 gggcttcgtg atcgaccgtg cgatcgaacc cctgcgggca ccggtgctgg tgcggctcga
      121 ttcccccgcc aacatcgccg ccggcgacac cgtgtggggc ctgggcgatc acctgcggca
      181 ccggttgcgc gccgccctgc ccggctgcac cctcctcacc gtccagaccc cggacgaact
      241 gaccgccgcc ctcgccgcgg ccgacggacg tcccctggtc gtgcagggcc gcgacatggc
      301 ccgcgtaccc ttcctctccg agtgcgcgac gaccgtgcgc aggcaccgtc cggacgcgct
      361 gctggtcgag ctcggctggc ccgaccccgc caccagcacc ccgcccgacc tggtcacctt
      421 cggcagcggc gccggccaga tgtcatgcct catcgacctg ctcgagcggg gcacccgatg
      481 agccccggca ccgagcaacc gaccaccctc ggcatcgaca tcggcggcac caagacccag
      541 ggcatcgtgc tcgacgggca ggcccgggtg atcgccgatc gccgtcggcg caccgcgacc
      601 ggcccggccg gggtgctcgc gacggccctg gccgtggccc gcgactgcct gcaactggcc
      661 ggcgtgcgcc aggaacacct gggctcggtc ggggtgggca ttccgggccg ggtcgatcac
      721 cgcaccggcg tggtcagcac cgccgtcaac ctgggcatcg gacggctcga gctcggctcc
      781 gcgctcagcg ccgaactcgg ggtgcccgtg caggtcgaca acgacgtgaa ggccaccgcg
      841 ctcggcgccg ccgactacct gcgggccggc gatgccgacc tgacctacct gaatctcggc
      901 accggcgtcg cctcggccac cctgatcggc ggacgcctgg tgcgcggcga cgacaacctc
      961 gccggcgaga tcgggcacgt gccgatcgac cccggcgccg gacgctgcgc ctgcggccag
     1021 cgcggctgca tcgaggtgct ggccggcggc gcacagatcg cgggccggct ggcggctctc
     1081 ggctcgggcc tcaccctgcg cacgttggtg agcgccgccg aggccggcga cccggccgcc
     1141 gacgacgagg tgcgacgcat cgccgaggcg atcgcgacgg ccgtccaact gctggtgctc
     1201 gcgcacggct cgacccgggt ggcgctgggg ggtggggtgg tgcacaccgc acccggactg
     1261 gtcgaacaga gccggcgggc gctcgaccgt cgcgccgagc actcgccctt cctgcgctcg
     1321 ctcgagttgt ccgcccggat caccctggtg ccggccgcac atccgctggc agcgatcgga
     1381 gccgctctgg tcggacgggc gggcctggcc cgggcgcact gggaacgccc tgcaagaatg
     1441 ggggcagcac cgcgcgaaag gcagccatga cccagggcaa caccccgcag tacccccagc
     1501 agcacgatcc gtaccggccg ggctacggcc agcccgggca gcccgaccag tcagaatggg
     1561 gccaaccggg cacccaggct ggctacggcg caggcggcca gcagccggcc ggctacccgc
     1621 agcaaagtta tccccagcag agttacccgc agcagggcta ttcgcaggac ggctaccagc
     1681 aggcctatcc gcagcagagt tacccccagc aggggtacca ggcgcccggc tacccggcgg
     1741 cctacccgca aacgatgccc tatggccagg cggggcccta cgcccaggcc gagcagccgc
     1801 gctcgccgct gctcggcatg atctcgctgg gcgtcgtggt gatctgcacg gtcatcttcg
     1861 gctgggtgat gttccgcatg ggtgcgttgc tggcgccggt gctggtgacc aacgcgggcg
     1921 ccgccacgca gcagcagatg acgcaggagc tgctcgatcg actcggtgcc acgggcctgc
     1981 tgctgctcaa tgtgtcgacc tacggcggct tcgcggcctg gatcaccggc atcgtcgcca
     2041 ccgccaccaa gcgtggccgc tcgtacggca tctgggccat catcatcggt gcgctcgcgc
     2101 cgttcatcgg cttcggcctg atgctggccg ccctgatgcc ctacatcaac gcctgagcag
     2161 gctcagctca gccgcccgac gcgtccgact cggcatcggc gatgtcggcc tcgcccaggc
     2221 agtccgaatc gagccagccg tagggcagca ccaccttgcc gcgcggtgcg ccctgccgtc
     2281 cgcgcggcga gcccagctcg gacgacgggt acggcacgtc cgcgtcgagg gtggccagca
     2341 ggccccgcag ttgtgccagg gactcgacgg tggccaggcc gcggcgcacg tcaccgccca
     2401 ccgcaaagcc cttgaaatac caggccatgt gcctgcgcag gtcgcgcagg ccgtgggtct
     2461 cgctgtccag accggccagg agttcggcgt gccggtagac catggccgcc acttcgccca
     2521 ggttcggcag cgggggcgcc tcccggcccg cgaacgcggc ggccaggtcg cggaacaacc
     2581 agggccggcc tagacagccg cgtccgatca ccacgccggc gcagccggtg cgccgcatca
     2641 tcgccagcgc gtccgcggcc tcccagatgt cgccgttgcc gagcaccggc aggtcgaccg
     2701 aggccaccag ttcggcgatc cgcgaccagt cggcctcgcc accgtaggcc tgtttgacgg
     2761 tgcgggcgtg cagggcgatc gccgcgcagc ccgcctcgcg cgcgatccgt ccggcgtcga
     2821 gaaaggtctc gtgctgctcg tcgatgccga ggcgggtctt catggtgacc ggcaccccgt
     2881 agggttcggc ggcccgcacc gttgccgtca ggatcgcggc gagccggtcg cgcttccacg
     2941 gcagcactcc cccaccgccg cgccgggtca ccttgggcac cgggcagccg aagttgaggt
     3001 cgacgtgcga caccccgaac tcgtcgcaca ggatcgtggt ggccttcgcc atcacggcgg
     3061 ggtcgacgcc gtacaactgc accgagcgga cggtctcgcc cgggtcgaag cgcagcatcg
     3121 cgaaggtctt ggggatgcgt tcgaccaccg cccggctggt gatcatctcg cacacgtaca
     3181 gcccggcgcc ctgctcgcga cacagctgcc ggtaggccgc gttggtgatg ccggccatgg
     3241 gggccagcac caccggcggg tcgaccgtga tcgggcccag gcgcagggtc tcggtctgcg
     3301 gggcggtctc ggtctgcagc gaacgcatcg gcccagccta accgtgccgg caccccccct
     3361 cctctcacgg gtcgaagcgg taccccagac ccggctcggt gatgaaatgt cgcggctcgc
     3421 cgggggcgtc ctcgagcttg cgccgcagct gggcgaagta gacccgcagg tagttgccct
     3481 ggttctcata gccggggccc cacacctcgg ccagcagttg cgcacccggc accaacgtgc
     3541 ccggacgccg caccagggcc tccaccaggt gccactcggt cggggtcagc ttcacctcgg
     3601 caccgtgccg cagggcccgc ttggcggcca ggtcgagggt gaacgagtcg gtgccgatca
     3661 ccggcagctc ggacggctcg ggcacgtccg cacgacgcaa cgcggcccgc acccgggcca
     3721 gcagctcgtc catgccgaac ggcttggtga cgtagtcgtc ggccccggcg tcgagtgcgc
     3781 gcaccttctg ctgctgggtg tcgcgggccg acagcacgat gatcggcacc cgcgaccacc
     3841 cgcgcagccc ggcgatcacc tcgaccccgt ccaggtcggg caggcccaga tcgaccagca
     3901 ccagctctgg cgggtgcgcc gcagcagc
//
LOCUS       DAOSUG010000282         3909 bp    DNA     linear   ENV 10-JUN-2024
DEFINITION  MAG TPA_asm: Micropruina sp. isolate AMR_MDS_2195
            NODE_9633_length_3909_cov_4.228594, whole genome shotgun sequence.
ACCESSION   DAOSUG010000282 DAOSUG010000000
VERSION     DAOSUG010000282.1
DBLINK      BioProject: PRJNA925477
            BioSample: SAMN32864262
            Sequence Read Archive: SRR4236673
KEYWORDS    WGS; ENV; Metagenome Assembled Genome; MAG; Third Party Data; TPA;
            TPA:assembly.
SOURCE      Micropruina sp. (wastewater metagenome)
  ORGANISM  Micropruina sp.
            Bacteria; Bacillati; Actinomycetota; Actinomycetes;
            Propionibacteriales; Nocardioidaceae; Micropruina.
REFERENCE   1  (bases 1 to 3909)
  AUTHORS   Abdulkadir,N., Saraiva,J.P., Zhang,J., Stolte,S., Gillor,O.,
            Harms,H. and Rocha,U.
  TITLE     Genome-centric analyses of 165 metagenomes show that mobile genetic
            elements are crucial for the transmission of antimicrobial
            resistance genes to pathogens in activated sludge and wastewater
  JOURNAL   Microbiol Spectr 12 (3), e0291823 (2024)
   PUBMED   38289113
REFERENCE   2  (bases 1 to 3909)
  AUTHORS   Abdulkadir,N.
  TITLE     Direct Submission
  JOURNAL   Submitted (24-MAR-2023) Environmental Microbiology, Helmholtz
            Center for Environmental Research -UFZ, Permoserstr 15, Leipzig
            04318, Germany
COMMENT     The annotation was added by the NCBI Prokaryotic Genome Annotation
            Pipeline (PGAP). Information about PGAP can be found here:
            https://www.ncbi.nlm.nih.gov/genome/annotation_prok/
            
            ##Genome-Assembly-Data-START##
            Assembly Method        :: SPAdes v. 3.11.1
            Genome Representation  :: Full
            Expected Final Version :: Yes
            Genome Coverage        :: Not Applicable
            Sequencing Technology  :: Illumina MiSeq
            ##Genome-Assembly-Data-END##
            
            ##Genome-Annotation-Data-START##
            Annotation Provider               :: NCBI
            Annotation Date                   :: 05/04/2023 04:57:10
            Annotation Pipeline               :: NCBI Prokaryotic Genome
                                                 Annotation Pipeline (PGAP)
            Annotation Method                 :: Best-placed reference protein
                                                 set; GeneMarkS-2+
            Annotation Software revision      :: 6.5
            Features Annotated                :: Gene; CDS; rRNA; tRNA; ncRNA
            Genes (total)                     :: 2,776
            CDSs (total)                      :: 2,750
            Genes (coding)                    :: 2,714
            CDSs (with protein)               :: 2,714
            Genes (RNA)                       :: 26
            tRNAs                             :: 24
            ncRNAs                            :: 2
            Pseudo Genes (total)              :: 36
            CDSs (without protein)            :: 36
            Pseudo Genes (ambiguous residues) :: 0 of 36
            Pseudo Genes (frameshifted)       :: 2 of 36
            Pseudo Genes (incomplete)         :: 32 of 36
            Pseudo Genes (internal stop)      :: 2 of 36
            ##Genome-Annotation-Data-END##
FEATURES             Location/Qualifiers
     source          1..3909
                     /organism="Micropruina sp."
                     /mol_type="genomic DNA"
                     /submitter_seqid="NODE_9633_length_3909_cov_4.228594"
                     /isolate="AMR_MDS_2195"
                     /isolation_source="Activated sludge of municipal
                     wastewater treatment plants"
                     /db_xref="taxon:2737536"
                     /environmental_sample
                     /geo_loc_name="Canada"
                     /lat_lon="49.25 N 123.1 W"
                     /collection_date="2015-06-16"
                     /metagenome_source="wastewater metagenome"
                     /note="metagenomic"
     gene            <1..1331
                     /locus_tag="PLF56_09525"
     CDS             <1..1331
                     /locus_tag="PLF56_09525"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_015069386.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_function: GO:0004803 - transposase activity [Evidence
                     IEA]"
                     /codon_start=3
                     /transl_table=11
                     /product="IS1380 family transposase"
                     /protein_id="HOQ53840.1"
                     /translation="LAVSGLASALSAGLEPWRKPQARHDPAKVLTDLALTLALGGDCL
                     ADAAVLRSEPDLFGPVASEATVSRTITALAAEAGRVLPAIAKARRAVRGRVWDLAGEE
                     SPLHAISETDPLVIDLDATIVISHSEKDGAAPTFKRTFGHHPLLAFLDHGAGGSGELL
                     AALLRPGNAGSNTAADHIEVTRQALAAVPGINASRPGRKVMIRTDGGGASKKFLTFLT
                     TRGLAYSIGWTLPWVEMNELYEIVTTAGGWSPALAEAGKVNEKADVADLTTLLKAAGH
                     LKGWPAGMRVIVRRERPHPGARVAQDRLDDADGYRLTAFATNARQGQLQVLELRHRQR
                     ARCEDRIRCAKDTGLRNLPLKTLAQNEIWLQVVALAGDLLAWLGLLGLKNETSRRWEP
                     KRLRLRLFSAPAVIARHARQVLLHVKETYAWASIVLAAHQRLRALAAAPT"
     gene            1719..>3909
                     /locus_tag="PLF56_09530"
     CDS             1719..>3909
                     /locus_tag="PLF56_09530"
                     /inference="COORDINATES: protein motif:HMM:NF013508.2"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="SpaA isopeptide-forming pilin-related protein"
                     /protein_id="HOQ53841.1"
                     /translation="MTATGDDGLVLSSTPGAQIKGSASGFTLTVTDANLSNVSFDSYQ
                     ISGLPDGWTLKAGGSTLKPVSPSPMIYEVPRSQTTLTAVAPANFVGTLQGVTIARVSD
                     ANNLVTDFDGGSFDYSGTAKPQLDSSVTAYTYKDPTTHAASYCNAVGQYAPCDGSYTI
                     WPTANLNGPDLPHLNNRWADLRSVKNALPDSGQTASALVCVNPTFGDYESDPAKANTY
                     QVSDADAVANGKFLIVNGSQSMPVPNDLIYTTLTDLAPNTTYTFSGYVANLSYDTRDT
                     KPVQSAFYLRSSPNDVGELVGSSQPMPKQAGCENSQTSWTKMTSSFTTTAGGEAIIGV
                     RNFGEGGHGNDLGIDQLSLTPMATVSTQLTVEPGSVSWAKVDAEGKPLDGSEWSLALE
                     GAGSLTVIDNGSNDADPGIGAILVNDLSAGSYTLTEVKAPVGHNLGDQAHTLVIDAEH
                     QHVSFGDIKNTRTPGSLSWSKTDAEDTATLLAGSEWTLVGPDGTSATIVDNGLNDEDD
                     VTGKLKVSDLAWGEYTLTETKAPEGYLLAAKPVTVTVDAQHTSINLDSVKNTKAVPGM
                     HVKKTSDPASGSRVNPGQEITYTVTVENTGNVDLTPAKLNDDLSDVLDNASYVENSAT
                     AKIGDADATTPTVEGTKLTWADRLNVGQSATITYKVKVNDNVTAKDKLVNVVTGEADV
                     PPGITPPTPNCVPDTAKENEDCTTNHTPNDPGMHVKKTSDPASGSRVN"
BASE COUNT          767 a         1344 c         1160 g          638 t
ORIGIN      
        1 cgttggcggt gagtggtcta gcgtctgcgc tctcggcggg gttggagccg tggcgtaagc
       61 cgcaagctcg gcatgacccg gccaaggtct tgaccgatct cgccctcacg ctcgccctgg
      121 ggggtgactg cctcgccgat gccgcggtgc tgcgcagtga gccggacctg ttcggtccgg
      181 tggcttcgga agcgacggtc tcgcgcacga tcaccgccct ggccgctgag gcgggtcggg
      241 tcctgcccgc gatcgcgaag gctcgtaggg cggtccgggg tcgggtctgg gatctcgcgg
      301 gtgaggagtc cccgctccac gcgatcagcg agactgaccc gttggtgatc gatctggatg
      361 ccacgatcgt gatcagtcac agcgagaaag acggcgccgc accgacgttc aagcgcacgt
      421 tcgggcatca cccgctgctg gcgttcctgg atcacggcgc cggtggcagt ggtgagctcc
      481 tcgccgccct gttgcggccc gggaacgccg ggtcgaacac ggcagcggat cacatcgagg
      541 tcacccggca ggcattggcc gccgtgccgg ggatcaacgc ctcccggccc ggacgcaaag
      601 tgatgatccg gaccgacggt ggtggggcga gcaagaagtt cctgacgttc ttgaccacca
      661 ggggcctggc ctactcgatc gggtggacgt tgccatgggt ggagatgaac gagctctacg
      721 agatcgtcac gaccgcgggt ggctggagcc cggccctggc cgaggcgggc aaggtcaacg
      781 agaaggccga cgtcgccgac ctcaccaccc tcctcaaagc tgcggggcac ctcaagggct
      841 ggccggccgg gatgcgcgtg atcgtgcgac gcgaacgacc ccaccccggt gcccgggtcg
      901 cgcaggaccg gctcgatgac gctgacggct accgcctgac cgcgtttgct accaacgccc
      961 gccaaggcca gctccaggtc ctcgagctcc gccaccgtca gcgggcccgc tgtgaggacc
     1021 ggatccgctg cgcgaaagac accgggctgc gcaacctccc gttgaaaacc ctggcgcaga
     1081 acgagatctg gctccaggtc gtcgctctcg ccggcgacct cctggcctgg ctcggcctcc
     1141 tcgggctgaa gaacgaaacc agccggcggt gggagccgaa acggttacgg ctacgcctgt
     1201 tcagcgcccc cgccgtgatc gcccgtcacg cccgacaagt gctcttgcac gtcaaagaga
     1261 cctacgcctg ggccagcatc gtgctcgccg cgcatcaacg cctacgggcc ctggccgccg
     1321 caccgacctg acgaaccatc gctcctgacc acccacgacc cgaggacccc acccggctgg
     1381 aaccggcacc caccgcagcg atacgcggcg caacgtcaca cccacaggac agaatcacga
     1441 ccagatgccg gcaccgacgc cgaccaactc atcgacctgg cccgatgaaa gatcgaggcc
     1501 agcttttgca gggccttaaa tctacactag tgtcaagaga tatgtccatc cggacattca
     1561 aaaatcgcat cctggggaga gaagtgacca cgagaagatc ggccaatgcc gcgcgccgcg
     1621 tgttggcaag tttcggtgta gcagctatcg ctgccatcgc acccttgaca gctgtgtcag
     1681 cgccggcttc tgccgcacca attctggccc cagctgccgt gacagccacc ggcgatgatg
     1741 gcctagtgct ttcgtcgacg cccggtgcgc agattaaggg ttctgccagc ggcttcacct
     1801 tgacggtcac tgacgccaac ctgagtaacg tctccttcga cagctaccaa atctctggcc
     1861 tccctgacgg gtggacgctc aaggctggcg ggagcaccct gaagccggtg tccccgagtc
     1921 cgatgattta cgaagtgccg cgctcccaga ccaccctgac ggccgtcgcc ccggcgaatt
     1981 tcgtcggtac gcttcagggc gtcacgattg cgcgtgtctc cgatgcgaac aacttggtga
     2041 ctgattttga cggtggttcc ttcgactact cgggtacggc caagccccag ctcgatagct
     2101 cggtcaccgc ctacacatac aaggacccca cgacgcacgc cgctagttac tgcaacgccg
     2161 ttggacagta cgccccttgt gatggctcct acaccatctg gcccactgcc aatctcaacg
     2221 gccccgatct gccgcatttg aacaaccgtt gggccgacct gcgcagtgtg aagaacgctc
     2281 tgcccgattc tggacagact gcttcggccc tggtgtgtgt caacccgact ttcggtgact
     2341 acgagtcaga ccccgccaag gcgaatacct atcaggtgag cgatgcggac gccgtggcga
     2401 acggcaagtt cctaatcgtg aacggttcgc agagcatgcc ggtcccgaac gatctgatct
     2461 acaccactct caccgacctg gcgccgaaca cgacctacac cttcagtggc tacgtggcca
     2521 acctctccta cgacactcgc gacactaagc ctgtgcagtc tgccttctac ttgaggtcct
     2581 cccccaacga tgtcggtgag ctagttggct cgtcgcagcc gatgcccaag caagctgggt
     2641 gcgagaactc acaaacctcg tggacgaaaa tgacttcctc cttcaccacc accgcaggcg
     2701 gcgaagcgat catcggcgtt cgcaacttcg gtgaaggcgg acacggcaac gacctgggca
     2761 tcgaccagct ctcactgacg cccatggcca ccgtctctac ccagctgacg gttgagcctg
     2821 gctctgtttc ttgggccaag gttgatgcag aggggaagcc cctggatggt tcggagtgga
     2881 gccttgccct cgagggtgct ggctccctga ccgtgatcga caacggttcc aacgatgcag
     2941 atccgggaat cggggcaatc ctcgtcaacg acctttccgc cggcagctac accctcacgg
     3001 aggtcaaggc ccccgtcggc cacaatctcg gcgaccaggc ccacactctt gtcatcgacg
     3061 cagagcacca gcatgtgagc ttcggagata tcaagaacac gcgtactccg ggtagcttgt
     3121 cgtggtcgaa gaccgatgcg gaagatacgg ccacgttgct tgctggctct gagtggacgc
     3181 tggtgggtcc cgatgggacc tccgccacga tcgtggacaa cggcctgaac gatgaggacg
     3241 acgtcaccgg caagctcaag gtcagcgacc tggcctgggg tgagtacacc ctcaccgaga
     3301 ccaaagcccc cgagggctac ttgctcgcgg ccaagccggt cacggtgacg gtcgatgcgc
     3361 agcacaccag catcaacctg gactccgtga agaatacgaa ggccgtgccg gggatgcatg
     3421 tgaagaagac ctccgacccg gcctccggtt cccgtgtgaa ccccggccag gagatcacct
     3481 acaccgtcac cgttgagaac accggcaacg tcgacctgac ccccgccaag ctcaacgacg
     3541 acctctccga cgtcctggac aacgccagct acgtcgagaa ctccgccacc gccaagatcg
     3601 gcgatgccga cgccaccacc ccgactgtgg agggaaccaa gctcacctgg gctgaccgac
     3661 tcaacgtcgg ccagagcgcg accatcacct acaaggtcaa ggtcaacgac aacgtcaccg
     3721 ccaaggacaa gctcgtcaac gtcgtcaccg gcgaagcaga cgtgcccccg ggcatcactc
     3781 ccccgacccc gaactgtgtc cccgacacag ccaaggaaaa cgaggactgc accaccaacc
     3841 acacccccaa tgacccgggg atgcatgtga agaagacctc cgacccggcc tccggttccc
     3901 gtgtgaacc
//
LOCUS       DAOSUG010000283         3906 bp    DNA     linear   ENV 10-JUN-2024
DEFINITION  MAG TPA_asm: Micropruina sp. isolate AMR_MDS_2195
            NODE_9643_length_3906_cov_4.307193, whole genome shotgun sequence.
ACCESSION   DAOSUG010000283 DAOSUG010000000
VERSION     DAOSUG010000283.1
DBLINK      BioProject: PRJNA925477
            BioSample: SAMN32864262
            Sequence Read Archive: SRR4236673
KEYWORDS    WGS; ENV; Metagenome Assembled Genome; MAG; Third Party Data; TPA;
            TPA:assembly.
SOURCE      Micropruina sp. (wastewater metagenome)
  ORGANISM  Micropruina sp.
            Bacteria; Bacillati; Actinomycetota; Actinomycetes;
            Propionibacteriales; Nocardioidaceae; Micropruina.
REFERENCE   1  (bases 1 to 3906)
  AUTHORS   Abdulkadir,N., Saraiva,J.P., Zhang,J., Stolte,S., Gillor,O.,
            Harms,H. and Rocha,U.
  TITLE     Genome-centric analyses of 165 metagenomes show that mobile genetic
            elements are crucial for the transmission of antimicrobial
            resistance genes to pathogens in activated sludge and wastewater
  JOURNAL   Microbiol Spectr 12 (3), e0291823 (2024)
   PUBMED   38289113
REFERENCE   2  (bases 1 to 3906)
  AUTHORS   Abdulkadir,N.
  TITLE     Direct Submission
  JOURNAL   Submitted (24-MAR-2023) Environmental Microbiology, Helmholtz
            Center for Environmental Research -UFZ, Permoserstr 15, Leipzig
            04318, Germany
COMMENT     The annotation was added by the NCBI Prokaryotic Genome Annotation
            Pipeline (PGAP). Information about PGAP can be found here:
            https://www.ncbi.nlm.nih.gov/genome/annotation_prok/
            
            ##Genome-Assembly-Data-START##
            Assembly Method        :: SPAdes v. 3.11.1
            Genome Representation  :: Full
            Expected Final Version :: Yes
            Genome Coverage        :: Not Applicable
            Sequencing Technology  :: Illumina MiSeq
            ##Genome-Assembly-Data-END##
            
            ##Genome-Annotation-Data-START##
            Annotation Provider               :: NCBI
            Annotation Date                   :: 05/04/2023 04:57:10
            Annotation Pipeline               :: NCBI Prokaryotic Genome
                                                 Annotation Pipeline (PGAP)
            Annotation Method                 :: Best-placed reference protein
                                                 set; GeneMarkS-2+
            Annotation Software revision      :: 6.5
            Features Annotated                :: Gene; CDS; rRNA; tRNA; ncRNA
            Genes (total)                     :: 2,776
            CDSs (total)                      :: 2,750
            Genes (coding)                    :: 2,714
            CDSs (with protein)               :: 2,714
            Genes (RNA)                       :: 26
            tRNAs                             :: 24
            ncRNAs                            :: 2
            Pseudo Genes (total)              :: 36
            CDSs (without protein)            :: 36
            Pseudo Genes (ambiguous residues) :: 0 of 36
            Pseudo Genes (frameshifted)       :: 2 of 36
            Pseudo Genes (incomplete)         :: 32 of 36
            Pseudo Genes (internal stop)      :: 2 of 36
            ##Genome-Annotation-Data-END##
FEATURES             Location/Qualifiers
     source          1..3906
                     /organism="Micropruina sp."
                     /mol_type="genomic DNA"
                     /submitter_seqid="NODE_9643_length_3906_cov_4.307193"
                     /isolate="AMR_MDS_2195"
                     /isolation_source="Activated sludge of municipal
                     wastewater treatment plants"
                     /db_xref="taxon:2737536"
                     /environmental_sample
                     /geo_loc_name="Canada"
                     /lat_lon="49.25 N 123.1 W"
                     /collection_date="2015-06-16"
                     /metagenome_source="wastewater metagenome"
                     /note="metagenomic"
     gene            <1..1176
                     /locus_tag="PLF56_09535"
     CDS             <1..1176
                     /locus_tag="PLF56_09535"
                     /inference="COORDINATES: protein motif:HMM:NF014177.2"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_function: GO:0009007 - site-specific
                     DNA-methyltransferase (adenine-specific) activity
                     [Evidence IEA];
                     GO_process: GO:0032775 - DNA methylation on adenine
                     [Evidence IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="DNA adenine methylase"
                     /protein_id="HOQ53842.1"
                     /translation="CARTNPRNERLTARRPEEPPARPRHPAPGTRHPASRVPEVIKYL
                     GSKRALVGVLGTLAEASGARTTVDLFTGTTRVAQEFKRRGLYVTAVDLASYSEILARC
                     YIGTDAAAVDLVELDALLAELDALPGVRGYITDTFCEQARFFQPHNGQRIDAIRARLA
                     GLVDHALYPVLLTALIEAADRVDSTTGQHMAYLKSWSKRSFNELRLRRPVLLDGPGEA
                     RRGAAQQVVTQLPPTDLMYLDPPYNQHRYFAYYHIWETLVRWDAPDHYGIARKRVDAR
                     AEQNQSPFNSRRTMAAAMADLIGNARASVLLVSYNDEAWVTPEAMIGWLREAGHQRVE
                     LLAFDSRRYIGAQIGIYNPQGERVGRVTHTRNTEYVFVAGEADRVEAAVRAAGPFIV"
     gene            1438..2208
                     /gene="cysE"
                     /locus_tag="PLF56_09540"
     CDS             1438..2208
                     /gene="cysE"
                     /locus_tag="PLF56_09540"
                     /EC_number="2.3.1.30"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_016454956.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_function: GO:0009001 - serine O-acetyltransferase
                     activity [Evidence IEA];
                     GO_process: GO:0006535 - cysteine biosynthetic process
                     from serine [Evidence IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="serine O-acetyltransferase"
                     /protein_id="HOQ53843.1"
                     /translation="MPHDEIWRRIRSEASDAAEAEPALASYLHTVVLAHDQLEDALSY
                     LLASKLGSSTITSLSLRSLIDEALGGDPGVGVAIREDLRAVLSRDPACLGYSIPLLHF
                     KGFHALQSYRVAHYFWLVGRKPLALYLQSRISEVFAVDIHPGARIGKGILFDHATSIV
                     IGETAVIEDDFSMLHEVTLGGTGKVGGDRHPKIRRGVMIGAGAKVLGNIEVGEGAKIG
                     AGSVVLDDVAPFTTVAGVPARPVSYPAHLFPEGLPHEI"
     gene            2347..3309
                     /locus_tag="PLF56_09545"
     CDS             2347..3309
                     /locus_tag="PLF56_09545"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_013863961.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="zinc-binding dehydrogenase"
                     /protein_id="HOQ53844.1"
                     /translation="MRAVYVETAQPDDPLAALKIGERPEPVPSDGWVVVEVEAAALNR
                     HDLWSLAGVGLPASRMPMILGTDAAGAVDGRPVLVHAVIATPGWVGDETLDPKRSLLS
                     ELHQGAMAQRVSVPRANLVDRPEWLSADEAACLPTAWLTAYRMLTHDSGLTAGDTVLV
                     QGASGGVASAAVALGRALEMRVWVTGRSEAKRAAALELGADAAFEPGTRLPERVDAVI
                     ETVGEATWAHSLRSLRPGGTIVCAGATSGFNPPAELNRVFFQQLRVIGSTMGTIGELQ
                     ALLQLLERTGIRPVIDRVLTLDDAADGFRAMAEGTLQGKIVIRP"
     gene            3671..>3906
                     /locus_tag="PLF56_09550"
     CDS             3671..>3906
                     /locus_tag="PLF56_09550"
                     /inference="COORDINATES: ab initio
                     prediction:GeneMarkS-2+"
                     /note="Derived by automated computational analysis using
                     gene prediction method: GeneMarkS-2+."
                     /codon_start=1
                     /transl_table=11
                     /product="hypothetical protein"
                     /protein_id="HOQ53845.1"
                     /translation="MPFAESDVALGVVNAELVVVRIKAERVDRLGREEYAFARVALRR
                     AGSPALGEVNDGVGNIGVAERDPHSGPSRLRTSLA"
BASE COUNT          610 a         1368 c         1310 g          618 t
ORIGIN      
        1 tgcgcgcgta cgaacccgcg caacgaacgc ttgaccgcgc gacgtccgga agagccaccc
       61 gcacgtcccc ggcacccggc acccggcacc cggcacccgg ccagtagggt gccggaggtg
      121 atcaagtacc tcggctccaa gcgcgcgctg gtcggggtgc tgggcacgct cgccgaggcg
      181 tccggagccc gaaccaccgt ggacctgttc accggcacca cccgggtggc gcaggagttc
      241 aagcgtcgtg gcctgtacgt gaccgccgtc gatctcgcca gctacagcga gatcctggcc
      301 cgttgctaca tcggcaccga cgccgccgcg gtcgacctcg tcgaactgga cgcgctgctg
      361 gccgagttgg acgccctgcc gggcgtgcgc ggctacatca ccgatacctt ctgcgagcag
      421 gccaggttct ttcagcctca caacggccaa cgcatcgacg cgatccgcgc gcggctggcc
      481 ggcctggtcg atcacgccct ctacccggtg ctgctcaccg cgttgatcga ggcggccgac
      541 cgcgtcgatt cgaccaccgg ccagcacatg gcgtacctga agtcgtggtc gaagcgctcg
      601 ttcaacgagc tgcggctgcg ccgtccggtg ctgctggacg gccccggcga ggcacggcgc
      661 ggggcggccc agcaggtggt cacacagttg cctccgaccg acctgatgta cctcgacccg
      721 ccctacaacc agcaccgcta cttcgcttac taccacatct gggagacgct ggtgcggtgg
      781 gacgcccccg atcactacgg catcgcacgc aagcgggtgg acgcccgcgc cgagcagaat
      841 cagagcccgt tcaactcgcg ccgcaccatg gccgccgcga tggccgacct gatcggcaac
      901 gccagggcgt cggtgctgct ggtgtcgtac aacgacgagg cctgggtcac ccccgaagcg
      961 atgatcggct ggctgcgcga ggccgggcat cagcgcgtcg aactgttggc cttcgactcg
     1021 cgccgctaca taggcgcgca gatcggcatc tacaacccgc agggcgagag ggtcggccgg
     1081 gtcacgcaca cgcgcaacac cgagtacgtc ttcgtggccg gcgaggccga tcgggtcgag
     1141 gccgcggtgc gggccgccgg gccgttcatc gtctgacccg gcggccgcgg tccacacgtc
     1201 gtccgaaaag gggactatgt cggactatgc cggctctttc ctgcgttgag cccagtagcg
     1261 aaccagccgg ctctcgcgca cgactcactg ctagattggc cgccatgcac accgtcgcca
     1321 cgaccagccg ccgcatcggc gcgctgctgt gcatgtgttg ttgtcgcctc ggctgagacc
     1381 tctcgccgtc caccccagca ggcccacaac atctcttcca cgcgtgaaag gctcgccatg
     1441 ccgcacgatg aaatctggcg ccgcatccgt tccgaagcct cggacgccgc cgaggccgaa
     1501 cccgccctgg ccagctatct acacacggtc gtgctcgccc acgaccaact cgaggacgcg
     1561 ctgagctacc tgctggcgtc caagctgggc agttcgacca tcacctcgct gagcctgcgg
     1621 tcgctgatcg acgaggcgct cggcggcgac ccgggcgtcg gggtcgccat tcgcgaagac
     1681 ctgcgtgcgg tgctcagccg cgacccggcc tgcctcgggt actcgattcc gttgctgcac
     1741 ttcaagggct tccacgcgct gcagtcgtac cgggtggccc actacttctg gctggtcgga
     1801 cgcaagccgc tcgcgctgta cctgcagagc cgcatctcgg aggtgttcgc ggtcgacatt
     1861 caccccggcg cacgcatcgg caagggcatt ctgttcgacc acgccacctc gatcgtgatc
     1921 ggcgagaccg cggtgatcga ggacgacttc tcgatgctgc acgaggtcac cctgggcggc
     1981 accggcaagg tcggcggcga ccggcacccc aagatccgtc gcggcgtgat gatcggcgcc
     2041 ggcgccaagg tgctcggcaa catcgaggtc ggcgaggggg cgaagatcgg cgccggttcg
     2101 gtcgtgctcg acgacgtggc accgttcacc acggtggcgg gggtgccggc tcggccggtc
     2161 agctacccgg cccacctgtt tcccgagggc ctgccgcacg agatctgacc gaaccccgcg
     2221 ggcgtccatc gtcacggcgc aagctcgcag gggcagatcg agccactgat cactcggcgg
     2281 cagcgacgac catccgggat cgcgggcggg ccagggagcg tccacccagg ggcgtaacct
     2341 gcgaacatgc gtgccgtcta tgtcgaaacc gcccagcccg acgatcccct cgcggcactg
     2401 aagatcggcg agcggcccga gccggtgccg tccgatggct gggtggtcgt cgaggtcgag
     2461 gcggcggcgc tgaaccggca cgacctgtgg tcgctggccg gggtcggcct gcccgccagt
     2521 cgcatgccga tgatcctcgg caccgatgcc gcgggtgcgg tcgacggacg tccggtgctc
     2581 gtccatgcgg tgatcgcaac gccgggttgg gtgggcgacg agaccctcga tccgaagcgt
     2641 tcgctgttgt cggagctgca tcagggtgcc atggctcagc gggtcagtgt gccgcgggcc
     2701 aacctggtcg accggcccga gtggctgtcg gccgacgagg cagcctgcct gcccacggcg
     2761 tggctgaccg cctaccgcat gctcacccac gactcggggc tgacggccgg cgacacggtg
     2821 ctggtgcagg gcgcctccgg tggggtggcg tcggcggcgg tcgccctggg acgggcgttg
     2881 gaaatgcgcg tgtgggtgac cggacgctcc gaggccaagc gcgcagccgc gcttgagctg
     2941 ggcgccgatg cggccttcga gccggggacc cggctgcccg aacgggtgga cgccgtgatc
     3001 gagaccgtcg gtgaggccac ctgggcgcat tcgctgcgct cgctgcgtcc gggcggcacg
     3061 atcgtctgtg ccggagccac cagcggtttc aacccgccgg ccgaattgaa ccgggtgttc
     3121 tttcagcagc tgcgggtgat cggctcgacg atgggcacga tcggtgaact acaggcgctg
     3181 ctgcagttgc tcgagcgcac cggcattcgc ccggtcatcg atcgggtact caccctggac
     3241 gatgccgccg acggttttcg cgccatggcc gagggcaccc tgcagggcaa gatcgtcatc
     3301 cggccctgag accgcaccgg ttcagggcct ggcgacacga tcgatcatca acgcgacccc
     3361 gcggtttgcc cgcgcgacca gcagttccac ctcgggatgg tcgatctgcc acaccggaac
     3421 agctcgtctg cgaacccttg gcccacgagc tcgactccag tgaagtcaag caccatctgg
     3481 cgaaactgct ccagcccctg cgcgaaccgc tttgcctgcg atcggccgag aaactcagaa
     3541 ccatgctgga cgagttgcac gcgcgggatg gtgcgcacga agtcaccgtc ctcggagaag
     3601 gctcgaatac tccgaccagg tccctcgtcg tgtccaaatc gagcgtgaac gtcaccacgg
     3661 tgcccaccgc atgcccttcg ccgagagcga tgtcgccctc ggcgttgtca acgctgaatt
     3721 ggtcgttgtt cgcatcaagg ctgaacgtgt cgaccgcctt ggacgtgaag aatatgcctt
     3781 cgcccgtgtg gcgttgcggc gcgctggttc gccggccctt ggtgaggtca acgacggcgt
     3841 cggcaacatc ggggtagccg aacgtgatcc tcactcgggg cctagcaggc ttcgtacgtc
     3901 gctcgc
//
LOCUS       DAOSUG010000284         3901 bp    DNA     linear   ENV 10-JUN-2024
DEFINITION  MAG TPA_asm: Micropruina sp. isolate AMR_MDS_2195
            NODE_9652_length_3901_cov_3.205148, whole genome shotgun sequence.
ACCESSION   DAOSUG010000284 DAOSUG010000000
VERSION     DAOSUG010000284.1
DBLINK      BioProject: PRJNA925477
            BioSample: SAMN32864262
            Sequence Read Archive: SRR4236673
KEYWORDS    WGS; ENV; Metagenome Assembled Genome; MAG; Third Party Data; TPA;
            TPA:assembly.
SOURCE      Micropruina sp. (wastewater metagenome)
  ORGANISM  Micropruina sp.
            Bacteria; Bacillati; Actinomycetota; Actinomycetes;
            Propionibacteriales; Nocardioidaceae; Micropruina.
REFERENCE   1  (bases 1 to 3901)
  AUTHORS   Abdulkadir,N., Saraiva,J.P., Zhang,J., Stolte,S., Gillor,O.,
            Harms,H. and Rocha,U.
  TITLE     Genome-centric analyses of 165 metagenomes show that mobile genetic
            elements are crucial for the transmission of antimicrobial
            resistance genes to pathogens in activated sludge and wastewater
  JOURNAL   Microbiol Spectr 12 (3), e0291823 (2024)
   PUBMED   38289113
REFERENCE   2  (bases 1 to 3901)
  AUTHORS   Abdulkadir,N.
  TITLE     Direct Submission
  JOURNAL   Submitted (24-MAR-2023) Environmental Microbiology, Helmholtz
            Center for Environmental Research -UFZ, Permoserstr 15, Leipzig
            04318, Germany
COMMENT     The annotation was added by the NCBI Prokaryotic Genome Annotation
            Pipeline (PGAP). Information about PGAP can be found here:
            https://www.ncbi.nlm.nih.gov/genome/annotation_prok/
            
            ##Genome-Assembly-Data-START##
            Assembly Method        :: SPAdes v. 3.11.1
            Genome Representation  :: Full
            Expected Final Version :: Yes
            Genome Coverage        :: Not Applicable
            Sequencing Technology  :: Illumina MiSeq
            ##Genome-Assembly-Data-END##
            
            ##Genome-Annotation-Data-START##
            Annotation Provider               :: NCBI
            Annotation Date                   :: 05/04/2023 04:57:10
            Annotation Pipeline               :: NCBI Prokaryotic Genome
                                                 Annotation Pipeline (PGAP)
            Annotation Method                 :: Best-placed reference protein
                                                 set; GeneMarkS-2+
            Annotation Software revision      :: 6.5
            Features Annotated                :: Gene; CDS; rRNA; tRNA; ncRNA
            Genes (total)                     :: 2,776
            CDSs (total)                      :: 2,750
            Genes (coding)                    :: 2,714
            CDSs (with protein)               :: 2,714
            Genes (RNA)                       :: 26
            tRNAs                             :: 24
            ncRNAs                            :: 2
            Pseudo Genes (total)              :: 36
            CDSs (without protein)            :: 36
            Pseudo Genes (ambiguous residues) :: 0 of 36
            Pseudo Genes (frameshifted)       :: 2 of 36
            Pseudo Genes (incomplete)         :: 32 of 36
            Pseudo Genes (internal stop)      :: 2 of 36
            ##Genome-Annotation-Data-END##
FEATURES             Location/Qualifiers
     source          1..3901
                     /organism="Micropruina sp."
                     /mol_type="genomic DNA"
                     /submitter_seqid="NODE_9652_length_3901_cov_3.205148"
                     /isolate="AMR_MDS_2195"
                     /isolation_source="Activated sludge of municipal
                     wastewater treatment plants"
                     /db_xref="taxon:2737536"
                     /environmental_sample
                     /geo_loc_name="Canada"
                     /lat_lon="49.25 N 123.1 W"
                     /collection_date="2015-06-16"
                     /metagenome_source="wastewater metagenome"
                     /note="metagenomic"
     gene            <1..1975
                     /locus_tag="PLF56_09555"
     CDS             <1..1975
                     /locus_tag="PLF56_09555"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_013864083.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=2
                     /transl_table=11
                     /product="tetratricopeptide repeat protein"
                     /protein_id="HOQ53846.1"
                     /translation="NALHRAYTRIGAGITTVPPAPLADPNKQLITDPVVPEEKRFCPN
                     CGEPIGRAIDGKPGRIAGYCPKCQTAYNFSPSIKAGELVAGQYEVAGALAYGGMGWIY
                     LARDKNVSDRWVVLKGLLNTGDPDTLRATLTEQQFLATVEHPLIVEIYNFVSHENNAY
                     IVMEYVGGRSLKQLLKQRMEANGGSYDPLPVDQALAFLIEILPAFSYLHELGLLYCDF
                     KPDNVIQVGDSLKLIDLGGVRRASDDESPIYGTVGYQAPEVALAGATVASDIFTIGRT
                     LMVLCAEVPEYQGDYEFTVPPAEQLPAFAQYDSLYRLLLKCCARDPGDRFDSAEDLRL
                     QMLGVLREVVSARGGGTATTSASSPLFEAPTVSSDRFDWRQLPQLRPDPSDPMAGWLD
                     SLSLSDPVERLGDLQRPPTVTTGVLLARCLAALESANQRVLDATVHTMLSDDPWEWRA
                     VWMAGLGALLRGDNATALSSFNAVYGQLPGELAPKLALAVACELAGQSEVAESLYRIC
                     AATDANYVTPAGFGLARLRAARGDLTGALGALELVPQTSRGYLESQKLTVEHLIALGK
                     DPAHLGEALKVLQHARLDEHSQLGYESAIYERALSLAGRGSVQLGSVTFTEGSLRDRL
                     ETGYRRLAQLSADPNERAELVDRANTIRVWSLL"
     gene            1972..3309
                     /locus_tag="PLF56_09560"
     CDS             1972..3309
                     /locus_tag="PLF56_09560"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_013864084.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="protein phosphatase 2C domain-containing
                     protein"
                     /protein_id="HOQ53847.1"
                     /translation="MNDDVRPTHPSSSPGTPAPPPAAVTPEAPAPGWPLWATDQRAGE
                     LAAEQQLAAESLAAAQEFSTLPLAAPCPQCGRPVRPDERYCENCGADVHPAVGAPAAS
                     AAPSEATRKLDRPQAARPTCLECGGQVDSDGYCQTCGTKAPSPRDHFEASPADWVGGV
                     CDRGLVHSRNEDAMALWAGTGPERTAVLVVCDGVTTSQDSDVASLAAAERARDVLAAS
                     QPSGLGTPDSQDAALAEALVRSAAEANEAVLATTAADSTNAASCTFAAAVVVDDRIYW
                     ASLGDSRVYFLGADSVVQLSTDDSVAEELIRAGSTRHAAENGPQAHAITRWLGRDSDD
                     IVPRTGVHPVRAEGWLVVCSDGLWNYASEPTTLGARLADAAAEGGTPTQIAGRMVRWA
                     NGQGGKDNVTVALARVQPLSAANLNEAPRATSTDSSGETVAAAQPESNGEEKN"
     gene            3309..>3901
                     /locus_tag="PLF56_09565"
     CDS             3309..>3901
                     /locus_tag="PLF56_09565"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_012923534.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="vWA domain-containing protein"
                     /protein_id="HOQ53848.1"
                     /translation="MALFTSAVYQNEFLPDGGTDVNAIVTIACSGAGAAGQTGSGDAG
                     EIIIVDTSGSMGERNMAAAKQAAMVALDHILDGTYFAVVAGNHKAYLAYPMVQSGAGM
                     VRMDARTRAEARRAISYFRADGGTAMGNWLNLARALFDSIGNLAQKHAILLTDGENHN
                     ETPQQLDAAIRNSIGQFQVDCRGVGVDWKVPEIRSIA"
BASE COUNT          596 a         1404 c         1348 g          553 t
ORIGIN      
        1 gaacgcgctg caccgcgcgt acacccgcat cggcgccggc atcacgacgg tgccgccggc
       61 cccgctcgcc gacccgaaca agcagctgat caccgacccg gtcgtgccgg aggagaagcg
      121 gttctgcccg aattgcggcg agccgatcgg acgcgcgatc gacggcaagc ccggacgcat
      181 cgccgggtac tgcccgaagt gccagacggc ctacaacttc tcgccctcga tcaaggccgg
      241 cgagctggtg gccgggcagt acgaggtggc gggcgcgctc gcgtacggcg gcatgggctg
      301 gatctacctg gcccgcgaca agaacgtctc ggaccgttgg gtggtgctca agggcctgct
      361 caacaccggc gatcccgaca cccttcgggc caccctgacc gagcagcagt tcctcgcgac
      421 cgtcgagcac cccctgatcg tcgagatcta caacttcgtc agccacgaga acaacgccta
      481 catcgtgatg gagtacgtcg gtggacgctc gctgaagcag ctgttgaagc agcgtatgga
      541 agccaacggc ggcagctacg acccgctccc ggtcgatcag gcgctggcgt ttctgatcga
      601 gatcctgcct gcgttcagct acctgcacga gctcggcctg ctgtactgcg atttcaagcc
      661 cgacaacgtg attcaggtcg gcgattcgct caagttgatc gacctgggcg gggtccgcag
      721 ggccagtgac gacgagtcgc cgatctacgg cacggtcggc taccaggccc ccgaggtggc
      781 cctcgccggc gccaccgtcg cctccgacat cttcaccatc ggacgcaccc tgatggtgct
      841 gtgtgccgag gtgcccgagt atcagggcga ctacgagttc accgttccgc cggccgagca
      901 attgccggcc ttcgctcagt acgactccct gtaccggctg ctgctcaagt gctgtgcccg
      961 tgaccccgga gaccgcttcg attcggccga ggatcttcgg ctgcagatgc tgggcgtgct
     1021 gcgcgaggtc gtctcggcgc gcggcggcgg caccgcgacc acctcggcgt cgtcgccgct
     1081 gttcgaggcg cctaccgtgt ccagtgaccg gttcgactgg cggcaactgc cccaactgcg
     1141 ccccgacccg tccgacccga tggccggctg gctcgactcg ctgagcctga gtgacccggt
     1201 ggagcggctc ggcgacctgc agcggccacc cacggtgacc accggggtgc tgctggcccg
     1261 ttgcctggcc gcgctcgaga gcgccaacca gcgggtgctc gacgcgaccg tgcacaccat
     1321 gctgagcgac gacccctggg agtggcgcgc ggtctggatg gccggcctgg gcgccctgct
     1381 gcgcggcgac aacgcgaccg cgctgtcctc gttcaacgcg gtgtacgggc aactgccggg
     1441 cgagttggcg cccaagctgg cgctcgcggt ggcctgtgag ctggccggcc agtccgaggt
     1501 cgccgagagc ctctaccgca tctgtgccgc caccgacgcc aactacgtga cgccggccgg
     1561 cttcggcctg gcccgccttc gcgcggcgcg cggcgacctg accggagcgc tcggggcgct
     1621 ggagctggtt ccgcagacct cgcggggcta cctggaatcg caaaagctga ccgtggagca
     1681 cctgatcgcc ctgggcaagg atccggccca cctcggcgag gcgctcaagg tgctgcagca
     1741 cgcccggctc gacgagcaca gccaactcgg ctacgagtcc gcgatctacg aacgggccct
     1801 gtcgctggcc ggccgcggct cggtccagtt gggttcggtc acgttcaccg aaggcagcct
     1861 gcgcgaccgg ctcgagacgg ggtaccggcg attggcccag ctgagcgccg acccgaacga
     1921 acgtgccgag ctggtcgacc gcgccaacac cattcgagtc tggagcctgc tgtgaacgac
     1981 gacgtccggc ccacgcaccc ctcctcctcg cccggcaccc ctgcgccgcc tccggctgcg
     2041 gtgacgcccg aggctcccgc gcccggctgg ccactgtggg ccaccgacca gcgggccggc
     2101 gaactggccg ccgagcagca actggcggcc gagtcgctgg ccgcggcgca ggagttctcc
     2161 accctgccgc tggcggcccc gtgcccacag tgcgggcgtc cggtgcgtcc ggacgagcgg
     2221 tactgcgaga actgcggcgc cgatgtgcac cccgccgtcg gggcgcccgc cgcgtccgct
     2281 gcgccctcgg aggcgacccg caagctcgac cggccgcagg ccgcccgtcc gacctgcctg
     2341 gaatgtggcg ggcaggtgga ctcggacggc tattgccaga cctgcggcac caaggccccc
     2401 tcgccacgcg accacttcga ggcctccccc gccgattggg tgggcggcgt ctgcgaccgg
     2461 ggtctcgtgc acagccgcaa cgaggacgcg atggcactgt gggccgggac cgggccggag
     2521 cgcaccgccg tcctggtggt gtgcgacggc gtgaccacct cgcaggactc cgacgtggcc
     2581 tcgctggctg cggccgagcg ggcccgcgac gtgctggcgg cttcgcagcc gtccgggctg
     2641 ggcacccccg acagccagga cgcggcgctc gccgaggccc tggtgcgctc ggcggcagag
     2701 gcgaacgagg cggtgctcgc cacgaccgcg gccgacagca ccaacgccgc ctcgtgcacc
     2761 ttcgccgcgg ccgtggtggt cgacgaccgc atttactggg cgtcgctcgg cgattcgcgg
     2821 gtctactttc tgggtgcgga ttccgtggtg cagttgagca ctgacgactc ggtcgccgaa
     2881 gaactgatca gggcaggatc gacacgccac gcggccgaga acggccccca ggcgcacgcg
     2941 atcacccgtt ggctgggccg cgactccgac gacatcgtgc cccgcacggg cgtccacccg
     3001 gtccgggccg agggctggct ggtggtctgc tcggacggcc tgtggaacta cgcgtccgag
     3061 ccgaccaccc tcggcgccag actcgccgac gcggctgccg agggcggcac gcccacccag
     3121 atcgcgggcc ggatggtgcg ctgggccaat ggccagggcg gcaaggacaa cgtgacggtc
     3181 gcactggcca gggtgcagcc gctgagtgcg gctaacctga acgaggcgcc gcgggcgacg
     3241 tcgacggatt cgtccggcga gacggtcgcg gcggcgcagc ctgaatcgaa cggagaagag
     3301 aagaactgat ggccctgttc acctctgcgg tgtaccagaa cgagtttctg cccgacggcg
     3361 gtaccgacgt caacgcgatc gtcacgatcg cgtgcagcgg ggcgggggcg gccggccaga
     3421 ccggcagcgg cgacgccggc gagatcatca tcgtcgacac ctccggctcg atgggtgaac
     3481 gcaacatggc ggccgccaag caggcggcca tggtggccct cgaccacatt ctcgacggca
     3541 cctacttcgc cgtggtggcc ggcaaccaca aggcctacct ggcctacccg atggtgcagt
     3601 ccggggccgg catggtgcgc atggatgcgc gcacccgcgc cgaggctcgg cgggcgatca
     3661 gctacttccg cgccgacggc ggcaccgcga tgggcaactg gctgaacctg gcccgggccc
     3721 tgttcgactc gatcggcaac ctggcgcaga agcacgccat tctgctcacc gacggcgaaa
     3781 accacaacga gacgccgcag caactcgacg ccgccattcg caactcgatc ggtcagtttc
     3841 aggtcgactg ccgaggcgtc ggggtcgatt ggaaggtgcc cgagattcgc agcatcgccc
     3901 a
//
LOCUS       DAOSUG010000285         3884 bp    DNA     linear   ENV 10-JUN-2024
DEFINITION  MAG TPA_asm: Micropruina sp. isolate AMR_MDS_2195
            NODE_9718_length_3884_cov_4.064508, whole genome shotgun sequence.
ACCESSION   DAOSUG010000285 DAOSUG010000000
VERSION     DAOSUG010000285.1
DBLINK      BioProject: PRJNA925477
            BioSample: SAMN32864262
            Sequence Read Archive: SRR4236673
KEYWORDS    WGS; ENV; Metagenome Assembled Genome; MAG; Third Party Data; TPA;
            TPA:assembly.
SOURCE      Micropruina sp. (wastewater metagenome)
  ORGANISM  Micropruina sp.
            Bacteria; Bacillati; Actinomycetota; Actinomycetes;
            Propionibacteriales; Nocardioidaceae; Micropruina.
REFERENCE   1  (bases 1 to 3884)
  AUTHORS   Abdulkadir,N., Saraiva,J.P., Zhang,J., Stolte,S., Gillor,O.,
            Harms,H. and Rocha,U.
  TITLE     Genome-centric analyses of 165 metagenomes show that mobile genetic
            elements are crucial for the transmission of antimicrobial
            resistance genes to pathogens in activated sludge and wastewater
  JOURNAL   Microbiol Spectr 12 (3), e0291823 (2024)
   PUBMED   38289113
REFERENCE   2  (bases 1 to 3884)
  AUTHORS   Abdulkadir,N.
  TITLE     Direct Submission
  JOURNAL   Submitted (24-MAR-2023) Environmental Microbiology, Helmholtz
            Center for Environmental Research -UFZ, Permoserstr 15, Leipzig
            04318, Germany
COMMENT     The annotation was added by the NCBI Prokaryotic Genome Annotation
            Pipeline (PGAP). Information about PGAP can be found here:
            https://www.ncbi.nlm.nih.gov/genome/annotation_prok/
            
            ##Genome-Assembly-Data-START##
            Assembly Method        :: SPAdes v. 3.11.1
            Genome Representation  :: Full
            Expected Final Version :: Yes
            Genome Coverage        :: Not Applicable
            Sequencing Technology  :: Illumina MiSeq
            ##Genome-Assembly-Data-END##
            
            ##Genome-Annotation-Data-START##
            Annotation Provider               :: NCBI
            Annotation Date                   :: 05/04/2023 04:57:10
            Annotation Pipeline               :: NCBI Prokaryotic Genome
                                                 Annotation Pipeline (PGAP)
            Annotation Method                 :: Best-placed reference protein
                                                 set; GeneMarkS-2+
            Annotation Software revision      :: 6.5
            Features Annotated                :: Gene; CDS; rRNA; tRNA; ncRNA
            Genes (total)                     :: 2,776
            CDSs (total)                      :: 2,750
            Genes (coding)                    :: 2,714
            CDSs (with protein)               :: 2,714
            Genes (RNA)                       :: 26
            tRNAs                             :: 24
            ncRNAs                            :: 2
            Pseudo Genes (total)              :: 36
            CDSs (without protein)            :: 36
            Pseudo Genes (ambiguous residues) :: 0 of 36
            Pseudo Genes (frameshifted)       :: 2 of 36
            Pseudo Genes (incomplete)         :: 32 of 36
            Pseudo Genes (internal stop)      :: 2 of 36
            ##Genome-Annotation-Data-END##
FEATURES             Location/Qualifiers
     source          1..3884
                     /organism="Micropruina sp."
                     /mol_type="genomic DNA"
                     /submitter_seqid="NODE_9718_length_3884_cov_4.064508"
                     /isolate="AMR_MDS_2195"
                     /isolation_source="Activated sludge of municipal
                     wastewater treatment plants"
                     /db_xref="taxon:2737536"
                     /environmental_sample
                     /geo_loc_name="Canada"
                     /lat_lon="49.25 N 123.1 W"
                     /collection_date="2015-06-16"
                     /metagenome_source="wastewater metagenome"
                     /note="metagenomic"
     gene            complement(<1..923)
                     /gene="pyk"
                     /locus_tag="PLF56_09570"
     CDS             complement(<1..923)
                     /gene="pyk"
                     /locus_tag="PLF56_09570"
                     /EC_number="2.7.1.40"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_014846912.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_component: GO:0005737 - cytoplasm [Evidence IEA];
                     GO_function: GO:0004743 - pyruvate kinase activity
                     [Evidence IEA];
                     GO_process: GO:0006096 - glycolytic process [Evidence
                     IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="pyruvate kinase"
                     /protein_id="HOQ53849.1"
                     /translation="MRRAKIVATLGPSTDTAEQIRGLIDAGVNVVRINMSHGTHPEHL
                     RRLKRVRDASNEAGRPIGVFADLQGPKIRLTLFENDFAILKAGQRFTITTDDVLGTAE
                     RAGTTLKTLTDDVRPGDKVLIDDGKIHLQALEVTDTEVITEVVVGGRVSNNKGINLPG
                     VAVSVPALSEKDEHDLRWALRHGIDMVALSFVRSASDYDRVREIMGDEDRHVPVIAKI
                     EKPQAVENLQEIIDTFDAIMVARGDLGVELPPEQVPSVQKRIIESARKWAKPVIVATQ
                     MLESMIGAPQPTRAETSDVANAILDGADAVML"
     gene            complement(983..1900)
                     /locus_tag="PLF56_09575"
     CDS             complement(983..1900)
                     /locus_tag="PLF56_09575"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_002527638.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_function: GO:0009982 - pseudouridine synthase activity
                     [Evidence IEA];
                     GO_process: GO:0001522 - pseudouridine synthesis [Evidence
                     IEA];
                     GO_process: GO:0009451 - RNA modification [Evidence IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="RluA family pseudouridine synthase"
                     /protein_id="HOQ53850.1"
                     /translation="MSVHLVPDGLAGERVDAAAARMTGLSRSRIDDLMAQGNVQLDGR
                     TVSKSTRVSGGELLEVTLPATPSGVPVVPRMVEGIRIVHDDADIVVVDKPAGVAAHPS
                     LGWDGPSVTEHLAAAGFRISTSGAAERQGVVQRLDVGTSGLMVVAKSERAYTLLKRAF
                     RDRTVDKVYHALVQGFPDPHVGTIDAPIGRHPGHDWKMAIIDGGRHSVTHYDTLEAFR
                     GATLLEVHLETGRTHQIRVHAAAIGHPCVGDPLYGGDPVLGARLGLQRQWLHAVELGF
                     EHPGSGELVRFASPYPDDLAHALAVLRSV"
     gene            complement(1897..2466)
                     /locus_tag="PLF56_09580"
     CDS             complement(1897..2466)
                     /locus_tag="PLF56_09580"
                     /EC_number="3.4.23.36"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_002513915.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_component: GO:0016020 - membrane [Evidence IEA];
                     GO_function: GO:0004190 - aspartic-type endopeptidase
                     activity [Evidence IEA];
                     GO_process: GO:0006508 - proteolysis [Evidence IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="signal peptidase II"
                     /protein_id="HOQ53851.1"
                     /translation="MPDLQAAGGTAIAGTTRRFALGRYALGALAIGVLGFLADLLTKQ
                     WALANLSPSAPVQVIPGQLYLYLIRNPGAAFSMGESFTVVLTCLAIAALCFLLFWMVP
                     RVGHPGWMVATGLLLAGVGGNLNDRLTREPGAFHGHVIDFLQVPWFAVFNVADMWITF
                     AAIAVIWLTMITSVNLAGQRVARSDRTAS"
     gene            complement(2430..2864)
                     /locus_tag="PLF56_09585"
     CDS             complement(2430..2864)
                     /locus_tag="PLF56_09585"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_015070022.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_function: GO:0008270 - zinc ion binding [Evidence IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="TraR/DksA C4-type zinc finger protein"
                     /protein_id="HOQ53852.1"
                     /translation="MPTARNAERVDTPSAAIPVAEGEEPWTADELAEVREELTGEVAR
                     LERAISIAEAGLKDLFSDGSEGAGRDPADVGSSNFERDQEMSLAQNAKEMLEQAQLAL
                     RLFDSGEYGTCESCGQPIGKDRLQVFPRATLCLTCKQREERR"
     gene            complement(2942..>3884)
                     /locus_tag="PLF56_09590"
     CDS             complement(2942..>3884)
                     /locus_tag="PLF56_09590"
                     /inference="COORDINATES: ab initio
                     prediction:GeneMarkS-2+"
                     /note="Derived by automated computational analysis using
                     gene prediction method: GeneMarkS-2+."
                     /codon_start=2
                     /transl_table=11
                     /product="hypothetical protein"
                     /protein_id="HOQ53853.1"
                     /translation="GEGGQAAPAAEDSGKVDTLQRQLNESHDEVQRLRSELDARTQEL
                     NGVRSELQGAQSALADGDQSGTVEKLQSELDQARSELEQARGELGGRDERIAGLEGEL
                     NGVRGELDTVTTAASERTGKVEHIVVTAAPDAAPAVTKLLQMATEQAERLVGEAEADA
                     QKLRGDAQADADRVTSDAGSTADATVEEASGKAHETLTAARSRAEEIEAQARSAADAL
                     SAETRARAEALKSEISERRAELFTSLESERDDLRGKVDHLRSFEQRYRDSLTTQLKAH
                     LDSLAGNAQPDDVPAVLDEPATQSATPRLDALLNDQN"
BASE COUNT          548 a         1333 c         1332 g          671 t
ORIGIN      
        1 agcatcaccg cgtcggcgcc gtccagaatg gcgttggcga catcggaggt ctcggcccgg
       61 gtcggctgcg gggctccgat catcgactcg agcatctggg tggccacgat caccggcttg
      121 gcccacttgc gggcgctctc gatgatgcgc ttctgcaccg acggcacctg ctcgggcggc
      181 agttcgacgc ccaggtcgcc gcgcgccacc atgatcgcgt cgaaagtgtc gatgatctct
      241 tgcaggttct cgaccgcctg cggcttctcg atcttggcga tcaccggtac atgccggtct
      301 tcatcgccca tgatctcgcg cacccggtcg tagtcggacg cactgcgcac gaacgacagc
      361 gccaccatgt cgatgccgtg ccgaagcgcc caccgcaggt cgtgctcgtc cttctcgctc
      421 agcgccggta ccgagactgc gacgccgggc aggttgatgc ccttgttgtt cgacacccgg
      481 ccgccgacca cgacctcggt gatcacctcg gtgtcggtga cctcgagcgc ctgcaggtga
      541 atcttgccgt cgtcgatcag caccttgtcg cccggccgga cgtcgtcggt gagggtcttc
      601 agggtggtgc cggcacgctc ggcggtgccc agcacgtcgt cggtggtgat ggtgaacctc
      661 tggccggcct tgaggatggc gaagtcgttc tcgaacagcg tcagccgaat cttgggccct
      721 tgcaggtcgg cgaacacccc gatcggacgg cccgcctcgt tggacgcatc gcgtacccgc
      781 ttgagccgcc gtagatgttc cggatgggtg ccgtgggaca tgttgatgcg caccacattc
      841 acgccggcgt cgatcaaccc cctgatctgt tccgccgtat cggttgaggg gccgagagtt
      901 gccacgatct ttgctctacg cacggaaccg aggttagcgg acgcagacga cgatgcgggg
      961 gcgatcgggt tacgcccagg attcagacgc tgcgcaacac cgcgagcgca tgtgccaagt
     1021 catcggggta cggcgaggcg aaccgcacga gctcgcccga acccgggtgc tcgaacccca
     1081 actcgaccgc atgcaaccat tgccgctgca ggccgagccg ggcgcccagc accgggtcgc
     1141 cgccgtacaa cgggtcaccc acgcaggggt ggccgatcgc ggcggcgtgc acgcggatct
     1201 gatgggtgcg tccggtttcg aggtgcacct cgagcagcgt cgcgccgcgg aaggcctcca
     1261 gggtgtcgta gtgggtcacg ctgtgccggc cgccgtcgat gatcgccatc ttccagtcat
     1321 gcccggggtg ccggccgatg ggggcgtcga tggtgccgac gtgagggtcg ggaaagccct
     1381 gcaccagcgc gtggtagacc ttgtcgacgg tgcggtcgcg gaacgcgcgc ttgagcaggg
     1441 tgtaggcccg ctcgctcttg gccaccacca tcagcccgga cgtgcccacg tcgagccgct
     1501 gcacgacgcc ctggcgttcg gccgcacccg aggtcgagat ccgaaagccc gcagcggcca
     1561 ggtgttcggt gaccgacggt ccgtcccagc ccagggacgg atgtgccgcc accccggcgg
     1621 gcttgtcgac caccacgatg tcggcatcgt catggacgat gcggatgccc tcgaccatcc
     1681 ggggcacgac cggcacaccg ctcggcgtgg ccggcagggt gacttcgagc agttctcctc
     1741 cgctgacccg ggtggacttg ctgaccgtgc gtccgtcgag ttgcacgttg ccctgggcca
     1801 tcaggtcgtc gatgcggctt cggctcaggc cggtcatgcg ggcggctgca gcgtcgaccc
     1861 gctcgccggc caggccgtcc ggcaccagat gaacgctcac gacgccgtgc ggtcggagcg
     1921 tgcgacccgc tggccggcca ggttcaccga ggtgatcatg gtcagccaga tcacggcgat
     1981 ggcggcgaag gtgatccaca tgtcggccac gttgaacacc gcgaaccacg gcacctgcag
     2041 aaagtcgatg acatggccgt ggaaggcgcc cggctcgcgg gtgagccggt cgttcaggtt
     2101 gccccccacg ccggccagca gcagcccggt cgcgaccatc cagcccgggt ggccgacccg
     2161 cggaaccatc cagaacaaca gaaagcagag ggctgcgatg gccaggcagg tgagcaccac
     2221 agtgaagctc tcgcccatgc tgaaggcggc cccggggttg cggatcagat acagatacaa
     2281 ctgcccgggg atcacctgca ccggcgcgct cgggctgagg tttgccagcg cccactgctt
     2341 cgtgagcagg tcggcgagga agccgagcac gccgatcgcc agcgccccca gcgcgtagcg
     2401 gcccagcgca aagcggcggg tggtgccggc tatcgccgtt cctcccgctg cttgcaggtc
     2461 aggcacaggg tcgccctcgg aaacacctgc agccggtcct tgccgatcgg ctgaccgcac
     2521 gattcgcacg ttccgtactc ccccgagtcg aacagccgca gcgccaactg ggcctgttcg
     2581 agcatctctt tcgcgttctg cgccagggac atctcctggt cacgttcgaa gttggacgag
     2641 cccacgtcgg ccgggtcgcg tccggcaccc tcgctgccgt cggagaacag gtctttcagg
     2701 cccgcttcgg cgatcgagat ggcgcgctcg aggcgggcca cctcaccggt gagttcctca
     2761 cggacctcag ccagctcgtc agccgtccag ggctcttcgc cctcggcgac cgggatggcc
     2821 gcggacgggg tgtccacccg ttccgcattg cgagccgttg gcatctggat tgtgctcctt
     2881 cagtcgtgtt cgagccgaga gagtaccacc cggaccggca ctgccgaagt aggacgcccc
     2941 gtcagttctg atcgttgagc agtgcgtcca gccgcggcgt tgccgactgc gtggccggct
     3001 cgtcgagcac cgcagggacg tcgtcgggct gggcgttgcc ggcgagcgaa tccaggtggg
     3061 ccttgagctg ggtggtcagg ctgtcgcggt agcgctgctc gaacgaacgc aggtggtcga
     3121 ccttgccgcg caggtcgtcg cgttcgcttt cgaggctggt gaacaactcg gcacggcgct
     3181 cgctgatctc gctcttcagc gcttcggccc tggcccgggt ctcggcactg agcgcgtcgg
     3241 cggcggaccg ggcctgggcc tcgatctctt cggcccgtga acgggccgcg gtgagggtct
     3301 cgtgcgcctt gccggaggcc tcttcgacgg tggcgtcggc cgtgctgccg gcgtcgctcg
     3361 tgacccggtc tgcgtcggcc tgggcatcgc cgcgcagctt ctgggcgtcg gcctcggcct
     3421 cgccgaccag gcgctcggcc tgctcggtgg ccatctgcag cagcttggtg accgcgggag
     3481 ccgcgtcggg ggctgcggtg accacgatgt gctccacctt gccggtgcgt tcgctcgcgg
     3541 ccgtggtgac cgtgtcgagc tcgccgcgta cgccgttcag ttcgccttcc aggccggcga
     3601 tgcgctcgtc gcgtccgccc aactcaccgc gcgcctgctc gagttcggag cgggcctgat
     3661 cgagttcgct ctgcaacttt tcgacggtac cgctctggtc gccgtcggcg agggcggact
     3721 gcgcgccctg cagctcgctg cggacgccgt tcagttcctg agtgcgtgcg tcgagttcgg
     3781 agcgcaggcg ctgaacctcg tcatgtgact cgttgagctg ccgttgcagg gtgtcgacct
     3841 tgcccgaatc ctcggcggcg ggagccgcct ggccgccctc gcca
//
LOCUS       DAOSUG010000286         3882 bp    DNA     linear   ENV 10-JUN-2024
DEFINITION  MAG TPA_asm: Micropruina sp. isolate AMR_MDS_2195
            NODE_9722_length_3882_cov_4.155213, whole genome shotgun sequence.
ACCESSION   DAOSUG010000286 DAOSUG010000000
VERSION     DAOSUG010000286.1
DBLINK      BioProject: PRJNA925477
            BioSample: SAMN32864262
            Sequence Read Archive: SRR4236673
KEYWORDS    WGS; ENV; Metagenome Assembled Genome; MAG; Third Party Data; TPA;
            TPA:assembly.
SOURCE      Micropruina sp. (wastewater metagenome)
  ORGANISM  Micropruina sp.
            Bacteria; Bacillati; Actinomycetota; Actinomycetes;
            Propionibacteriales; Nocardioidaceae; Micropruina.
REFERENCE   1  (bases 1 to 3882)
  AUTHORS   Abdulkadir,N., Saraiva,J.P., Zhang,J., Stolte,S., Gillor,O.,
            Harms,H. and Rocha,U.
  TITLE     Genome-centric analyses of 165 metagenomes show that mobile genetic
            elements are crucial for the transmission of antimicrobial
            resistance genes to pathogens in activated sludge and wastewater
  JOURNAL   Microbiol Spectr 12 (3), e0291823 (2024)
   PUBMED   38289113
REFERENCE   2  (bases 1 to 3882)
  AUTHORS   Abdulkadir,N.
  TITLE     Direct Submission
  JOURNAL   Submitted (24-MAR-2023) Environmental Microbiology, Helmholtz
            Center for Environmental Research -UFZ, Permoserstr 15, Leipzig
            04318, Germany
COMMENT     The annotation was added by the NCBI Prokaryotic Genome Annotation
            Pipeline (PGAP). Information about PGAP can be found here:
            https://www.ncbi.nlm.nih.gov/genome/annotation_prok/
            
            ##Genome-Assembly-Data-START##
            Assembly Method        :: SPAdes v. 3.11.1
            Genome Representation  :: Full
            Expected Final Version :: Yes
            Genome Coverage        :: Not Applicable
            Sequencing Technology  :: Illumina MiSeq
            ##Genome-Assembly-Data-END##
            
            ##Genome-Annotation-Data-START##
            Annotation Provider               :: NCBI
            Annotation Date                   :: 05/04/2023 04:57:10
            Annotation Pipeline               :: NCBI Prokaryotic Genome
                                                 Annotation Pipeline (PGAP)
            Annotation Method                 :: Best-placed reference protein
                                                 set; GeneMarkS-2+
            Annotation Software revision      :: 6.5
            Features Annotated                :: Gene; CDS; rRNA; tRNA; ncRNA
            Genes (total)                     :: 2,776
            CDSs (total)                      :: 2,750
            Genes (coding)                    :: 2,714
            CDSs (with protein)               :: 2,714
            Genes (RNA)                       :: 26
            tRNAs                             :: 24
            ncRNAs                            :: 2
            Pseudo Genes (total)              :: 36
            CDSs (without protein)            :: 36
            Pseudo Genes (ambiguous residues) :: 0 of 36
            Pseudo Genes (frameshifted)       :: 2 of 36
            Pseudo Genes (incomplete)         :: 32 of 36
            Pseudo Genes (internal stop)      :: 2 of 36
            ##Genome-Annotation-Data-END##
FEATURES             Location/Qualifiers
     source          1..3882
                     /organism="Micropruina sp."
                     /mol_type="genomic DNA"
                     /submitter_seqid="NODE_9722_length_3882_cov_4.155213"
                     /isolate="AMR_MDS_2195"
                     /isolation_source="Activated sludge of municipal
                     wastewater treatment plants"
                     /db_xref="taxon:2737536"
                     /environmental_sample
                     /geo_loc_name="Canada"
                     /lat_lon="49.25 N 123.1 W"
                     /collection_date="2015-06-16"
                     /metagenome_source="wastewater metagenome"
                     /note="metagenomic"
     gene            complement(<1..1119)
                     /locus_tag="PLF56_09595"
     CDS             complement(<1..1119)
                     /locus_tag="PLF56_09595"
                     /inference="COORDINATES: ab initio
                     prediction:GeneMarkS-2+"
                     /note="Derived by automated computational analysis using
                     gene prediction method: GeneMarkS-2+."
                     /codon_start=1
                     /transl_table=11
                     /product="hypothetical protein"
                     /protein_id="HOQ53854.1"
                     /translation="MWEQIGELFGDLAAAGGQIVVDAWVAICMAVWGAGLWVMRAILQ
                     LGDWFLTPNLNPDGSSSALWSVYGLTLWLGLSLALMLLIVQLVGTVLRRDALSLGGAI
                     FGAVKFLLVCALWYTYGAALVTAVEGLNTALRIQLLGTADLADWEPTGLDFAEGVMTG
                     TTATVLMLLGLVLWLAALAHLVVMLGRAVSLIVIAATAPIAAAGLMYKPFESWFWKAF
                     RWFHAAAIAPLLMTLMLGLGVQLATGVSLGLEDSAQEAVALALPSVVMIAVAALSPVA
                     LFRLLAFVDPGTTSGASFRAGLAGTTGASSAAGHAHTAVSRSGGSGGGSGGGGGAGES
                     ATASRAAAAQSSSLSPGAAGAGGGGAGGAAGGAAGGSAG"
     gene            complement(1121..1891)
                     /locus_tag="PLF56_09600"
     CDS             complement(1121..1891)
                     /locus_tag="PLF56_09600"
                     /inference="COORDINATES: ab initio
                     prediction:GeneMarkS-2+"
                     /note="Derived by automated computational analysis using
                     gene prediction method: GeneMarkS-2+."
                     /codon_start=1
                     /transl_table=11
                     /product="hypothetical protein"
                     /protein_id="HOQ53855.1"
                     /translation="MRPDRAHLTLLAAVTSVALLLLSGLGFAVYFAVADDPTTPTTAR
                     VVDSLPAGGVTVRDEIAAAPMLPVSAAAATGGTPALGTLPTIDIPVVDQVGAEGIPTG
                     YPQTPEGAVGQLGEILVSVLGAMDLTHAQRVQQSWFQDPAGSAASWPVLGLIQSFLTA
                     GRMPAGLEPGASLTVVPAAGLVKGSDGDGWHVVCVLVEITYTFRDQARLAYGHCERMT
                     WQEDRWVIGAGAHPVPGPSTWPGTELAVKAGWLQWRDA"
     gene            complement(1894..2226)
                     /locus_tag="PLF56_09605"
     CDS             complement(1894..2226)
                     /locus_tag="PLF56_09605"
                     /inference="COORDINATES: ab initio
                     prediction:GeneMarkS-2+"
                     /note="Derived by automated computational analysis using
                     gene prediction method: GeneMarkS-2+."
                     /codon_start=1
                     /transl_table=11
                     /product="hypothetical protein"
                     /protein_id="HOQ53856.1"
                     /translation="MIVQFVAEAMSAGVVVPAEGRGMVCPVAPAGAQVLVDEFLGWFS
                     WALVWVAFPAGILAAVAAVILGKVFSMPHVSKGGLIGVFVVLGAALLYLVIPGILSGF
                     LGDGCVTR"
     gene            complement(2223..2960)
                     /locus_tag="PLF56_09610"
     CDS             complement(2223..2960)
                     /locus_tag="PLF56_09610"
                     /inference="COORDINATES: ab initio
                     prediction:GeneMarkS-2+"
                     /note="Derived by automated computational analysis using
                     gene prediction method: GeneMarkS-2+."
                     /codon_start=1
                     /transl_table=11
                     /product="hypothetical protein"
                     /protein_id="HOQ53857.1"
                     /translation="MTTATFSLAELRAAYTALHAGAFTTDPTPQPETATAAVAELPAA
                     AADAVVSVVGVSGGVGTTTVALAIAEALGAGRLVELTAPHTSGLAAASSAELGTQHGW
                     SIGTRDGLRLERRTDPDAAILPDTAAGVTVLDLGAWTEPLPAVSVLVVVAACSVPSVR
                     RLNARLEVLHGLRVVPVITGVPGRALPKPVGSVCGARLRAAAADGRLLLVPECASLRV
                     GGVAADPLPRRLRSAVDVIKSCVEEIS"
     gene            complement(2957..3127)
                     /locus_tag="PLF56_09615"
     CDS             complement(2957..3127)
                     /locus_tag="PLF56_09615"
                     /inference="COORDINATES: ab initio
                     prediction:GeneMarkS-2+"
                     /note="Derived by automated computational analysis using
                     gene prediction method: GeneMarkS-2+."
                     /codon_start=1
                     /transl_table=11
                     /product="hypothetical protein"
                     /protein_id="HOQ53858.1"
                     /translation="MSDDETQQLIAAAKGEAEACDQLVFWTQRLQHALDARRQLLDVP
                     PEELKAALEAQR"
     gene            3354..3827
                     /locus_tag="PLF56_09620"
     CDS             3354..3827
                     /locus_tag="PLF56_09620"
                     /inference="COORDINATES: ab initio
                     prediction:GeneMarkS-2+"
                     /note="Derived by automated computational analysis using
                     gene prediction method: GeneMarkS-2+."
                     /codon_start=1
                     /transl_table=11
                     /product="hypothetical protein"
                     /protein_id="HOQ53859.1"
                     /translation="MRLFIDESYGTDDYYVAGVLVTEAQYRNLVDGLEEVRQHARQKF
                     GVGPEVEFHGHQIMQAQGEWTCMSSQLHEAVALYRNVLRAIVGSKATVLLEGVDVRRL
                     NARYRYPDSPYEVTLRHLLERADSWCASWGVTCQVTADLIDRQQDFWSVPEKMET"
BASE COUNT          701 a         1407 c         1272 g          502 t
ORIGIN      
        1 accagcggag ccgcccgcgg ctcccccggc ggctccaccg gctccgccgc cgccggcgcc
       61 cgctgcgccg gggctgaggc tggagctctg cgccgccgcg gcacggctcg ccgtggccga
      121 ctcccccgca ccaccaccac caccagaacc gccgccactg ccgccgctgc gggagactgc
      181 ggtgtgggcg tggccggcgg ccgaggaggc gccggtggtg ccggcgaggc ccgcacggaa
      241 cgaggcgccg ctggtggtgc ccggatcaac gaacgcaagc agccggaaca gggccaccgg
      301 ggacaacgct gcgacggcga tcatcacaac actcggcaac gcgagggcga cggcttcctg
      361 ggcggagtcc tccaatccga gggacacgcc ggtggcgagc tgcaccccca gcccgagcat
      421 gagcgtcatc agcagcgggg cgatggcggc ggcgtggaac cagcgaaacg ccttccagaa
      481 ccaggattcg aacggcttgt acatcaggcc ggctgcggcg atgggggcgg tggcggcgat
      541 cacgatcagg gacaccgccc ggccaagcat caccaccaga tgcgccaacg cggccagcca
      601 caacaccaac cccagcaaca tgagcacggt ggcggtggtg ccggtcatca ccccttcggc
      661 aaaatccaga ccggtaggct cccagtcggc aagatccgct gtgcccagca gctggatccg
      721 cagcgccgtg ttgagtccct ccacagccgt gaccagggcg gcgccgtagg tgtaccagag
      781 ggcgcagacc agcagaaact tcaccgcacc gaagatggcg ccgccaaggg agagggcgtc
      841 tcggcggagg acggtgccga ccagctgcac gatcagcagc atcaacgcca acgagagacc
      901 gagccagagg gtgaggccgt agaccgacca cagcgccgac gaggacccgt cggggttgag
      961 gttgggggtg aggaaccagt cccccagctg caggatcgca cgcatcaccc acaacccggc
     1021 cccccacacc gccatacaga tcgccaccca cgcgtccacc acaatctgac ccccggccgc
     1081 ggccagatcg ccgaacaact ccccgatctg ctcccacatc tcaggcgtcc cgccattgca
     1141 gccagccagc tttgacggcg agttcggtgc cgggccacgt cgatggcccc ggcaccggat
     1201 gcgcccccgc cccgatgacc cagcggtctt cttgccaggt catgcgttcg caatgcccgt
     1261 aggccaggcg ggcctggtcg cggaacgtgt aggtgatctc caccaacaca cacaccacat
     1321 gccaaccgtc gccgtccgag cccttcacca agccggcagc cgggaccacg gtcagcgatg
     1381 cccccggctc caaaccagcc ggcattctcc cggcggtcag gaacgactgg atcagcccca
     1441 gcaccggcca ggacgcggcc gatccggcgg ggtcttggaa ccaggactgc tgcacgcgct
     1501 gcgcatgggt caggtccatg gcgccaagga cggagacgag gatctccccc aactgcccca
     1561 ccgcgccctc tggcgtctgc gggtagccgg tcgggatccc ctccgccccg acctgatcca
     1621 ccacagggat gtcgatcgtc ggcagggtgc cgagggccgg ggtgccgccc gtggcagctg
     1681 ccgcagacac cggcagcatc ggggcggccg cgatctcgtc acgcaccgtg acgccgccgg
     1741 ccggtagcga gtcgacgacc ctcgccgtcg tgggggtggt ggggtcgtcg gcgacggcga
     1801 agtagacggc gaaccccaga ccggagagca gcagcaacgc caccgacgtg acggcggcca
     1861 gcagggtcag gtgcgcgcga tcgggcctca tggttagcgg gtgacgcagc cgtcgccgag
     1921 gaaccccgac aagatgcccg ggatcacgag gtagagcagt gcggcgccga ggacgacgaa
     1981 caccccgatc aggccgccct tcgacacgtg cggcatcgag aacaccttgc ccaggatcac
     2041 cgccgccacc gcggccagga ttcccgccgg gaacgccacc cacaccagcg cccacgagaa
     2101 ccagcccagg aactcatcga ccaggacctg cgcccccgcc ggcgcgaccg ggcacaccat
     2161 cccccgcccc tccgccggca ccaccacacc cgcagacatg gcctcggcta cgaactgaac
     2221 gatcatgaga tttcctccac acacgacttg atgacatcga ccgcagaccg cagccgacga
     2281 ggaagcggat cggcagcaac cccaccgacc cgaagagacg cacattccgg caccagcagc
     2341 agccgcccgt cggccgctgc ggcacgcagc ctcgccccgc acaccgaacc gaccggcttg
     2401 ggcagtgccc gacccgggac cccggtgatc accggcacga cccgcaggcc gtggaggacc
     2461 tcaaggcggg cgttgagacg ccgcacgctc ggcaccgaac acgccgccac caccaccaac
     2521 acagacaccg ccggcagagg ctcggtccaa gcgccgaggt ccaggaccgt gacccctgcg
     2581 gcggtgtcgg ggaggatcgc agcgtcgggg tcggtgcgac gctccagccg cagcccgtcg
     2641 cgggtcccga tgctccagcc gtgctgggtg ccgagttcag ccgacgacgc ggccgccaag
     2701 ccagaggtgt gcggcgcggt gagctcgacc aggcggccgg cgccgagtgc ttcggcgatc
     2761 gccaacgcca ccgtggtcgt ccccacccca cccgagacac cgaccacact caccaccgca
     2821 tccgcagccg cagctggcag ttccgccact gctgcggtgg ccgtttcggg ttggggggtc
     2881 gggtcggtgg tgaaggctcc ggcgtggagg gcggtgtagg cggcgcgcag ctcagccagc
     2941 gagaacgtgg cggtggtcat cgctgcgcct ccagggcggc cttcagttcc tccgggggaa
     3001 cgtccagcag ctggcggcgg gcatccagcg cgtgctgcag gcgctgggtc cagaacacca
     3061 actggtcgca ggcctccgcc tcacccttcg cagcagcaat cagctgctgt gtctcgtcat
     3121 ccgacatctc cgtggccttt cgctgtcctc gtgctgtcct gccccctatg aacccggacc
     3181 catcccgggc agcgcgtgaa gagatttcga ggtgctgtgt gccaggtcga gtccgctcga
     3241 ggggtggtct ctgatcaccg ctcgggcatg cgctccgggg ccactacccg acaaatctgg
     3301 ccttagaggt gcggggtgag agactcgtgg gcgtggccgc gcgagacgtt cagatgagac
     3361 tgttcattga cgagtcgtac ggcaccgatg actactacgt ggcaggtgtt ctggtcaccg
     3421 aggcccagta ccggaacctg gttgacggcc tggaggaggt tcgtcagcac gcccgtcaga
     3481 agttcggggt ggggccagag gtcgagttcc acggccatca gatcatgcag gcccagggtg
     3541 agtggacgtg catgtcgtca cagctccatg aggcagtcgc cctctaccgc aacgtactcc
     3601 gggcgatcgt cggctcgaaa gccaccgtcc tgctcgaagg agtcgacgtg cgtcgcctca
     3661 acgcccgcta ccgctacccc gactccccct acgaagtcac cctgcggcac ctgctggaac
     3721 gagcggacag ctggtgcgcc agctggggtg tcacctgcca ggtcaccgcc gacctgatcg
     3781 accgccaaca ggacttctgg agtgttcccg aaaaaatgga gacgtagttt atgccgcgag
     3841 ttcgtggatg tgtctgaatc ctagctcgta ttcgtgtggt gt
//
LOCUS       DAOSUG010000287         3876 bp    DNA     linear   ENV 10-JUN-2024
DEFINITION  MAG TPA_asm: Micropruina sp. isolate AMR_MDS_2195
            NODE_9746_length_3876_cov_4.065166, whole genome shotgun sequence.
ACCESSION   DAOSUG010000287 DAOSUG010000000
VERSION     DAOSUG010000287.1
DBLINK      BioProject: PRJNA925477
            BioSample: SAMN32864262
            Sequence Read Archive: SRR4236673
KEYWORDS    WGS; ENV; Metagenome Assembled Genome; MAG; Third Party Data; TPA;
            TPA:assembly.
SOURCE      Micropruina sp. (wastewater metagenome)
  ORGANISM  Micropruina sp.
            Bacteria; Bacillati; Actinomycetota; Actinomycetes;
            Propionibacteriales; Nocardioidaceae; Micropruina.
REFERENCE   1  (bases 1 to 3876)
  AUTHORS   Abdulkadir,N., Saraiva,J.P., Zhang,J., Stolte,S., Gillor,O.,
            Harms,H. and Rocha,U.
  TITLE     Genome-centric analyses of 165 metagenomes show that mobile genetic
            elements are crucial for the transmission of antimicrobial
            resistance genes to pathogens in activated sludge and wastewater
  JOURNAL   Microbiol Spectr 12 (3), e0291823 (2024)
   PUBMED   38289113
REFERENCE   2  (bases 1 to 3876)
  AUTHORS   Abdulkadir,N.
  TITLE     Direct Submission
  JOURNAL   Submitted (24-MAR-2023) Environmental Microbiology, Helmholtz
            Center for Environmental Research -UFZ, Permoserstr 15, Leipzig
            04318, Germany
COMMENT     The annotation was added by the NCBI Prokaryotic Genome Annotation
            Pipeline (PGAP). Information about PGAP can be found here:
            https://www.ncbi.nlm.nih.gov/genome/annotation_prok/
            
            ##Genome-Assembly-Data-START##
            Assembly Method        :: SPAdes v. 3.11.1
            Genome Representation  :: Full
            Expected Final Version :: Yes
            Genome Coverage        :: Not Applicable
            Sequencing Technology  :: Illumina MiSeq
            ##Genome-Assembly-Data-END##
            
            ##Genome-Annotation-Data-START##
            Annotation Provider               :: NCBI
            Annotation Date                   :: 05/04/2023 04:57:10
            Annotation Pipeline               :: NCBI Prokaryotic Genome
                                                 Annotation Pipeline (PGAP)
            Annotation Method                 :: Best-placed reference protein
                                                 set; GeneMarkS-2+
            Annotation Software revision      :: 6.5
            Features Annotated                :: Gene; CDS; rRNA; tRNA; ncRNA
            Genes (total)                     :: 2,776
            CDSs (total)                      :: 2,750
            Genes (coding)                    :: 2,714
            CDSs (with protein)               :: 2,714
            Genes (RNA)                       :: 26
            tRNAs                             :: 24
            ncRNAs                            :: 2
            Pseudo Genes (total)              :: 36
            CDSs (without protein)            :: 36
            Pseudo Genes (ambiguous residues) :: 0 of 36
            Pseudo Genes (frameshifted)       :: 2 of 36
            Pseudo Genes (incomplete)         :: 32 of 36
            Pseudo Genes (internal stop)      :: 2 of 36
            ##Genome-Annotation-Data-END##
FEATURES             Location/Qualifiers
     source          1..3876
                     /organism="Micropruina sp."
                     /mol_type="genomic DNA"
                     /submitter_seqid="NODE_9746_length_3876_cov_4.065166"
                     /isolate="AMR_MDS_2195"
                     /isolation_source="Activated sludge of municipal
                     wastewater treatment plants"
                     /db_xref="taxon:2737536"
                     /environmental_sample
                     /geo_loc_name="Canada"
                     /lat_lon="49.25 N 123.1 W"
                     /collection_date="2015-06-16"
                     /metagenome_source="wastewater metagenome"
                     /note="metagenomic"
     gene            complement(<1..587)
                     /locus_tag="PLF56_09625"
     CDS             complement(<1..587)
                     /locus_tag="PLF56_09625"
                     /inference="COORDINATES: protein motif:HMM:NF012334.2"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="SDR family NAD(P)-dependent oxidoreductase"
                     /protein_id="HOQ53860.1"
                     /translation="MSVVAVFGGRSEIGLEIARRLAEGNTVVLASRAGGDRPEVPGAE
                     RVLHVDFDADDVAAHAATVAAIESEVGPIDIAVLTFGILGDQARAETDPGEVVRIIHT
                     DFTAQASLLTVLAGLMRPRGTGTIVAFSSVAGQRVRRANYVYGSAKAGLDGFASGMAD
                     ALHGTGVDLIIARPGFVIGRMTAGMSPAPFSSTPE"
     gene            complement(601..2082)
                     /gene="gltX"
                     /locus_tag="PLF56_09630"
     CDS             complement(601..2082)
                     /gene="gltX"
                     /locus_tag="PLF56_09630"
                     /EC_number="6.1.1.17"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_014847264.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_component: GO:0009332 - glutamate-tRNA ligase complex
                     [Evidence IEA];
                     GO_function: GO:0004818 - glutamate-tRNA ligase activity
                     [Evidence IEA];
                     GO_process: GO:0006424 - glutamyl-tRNA aminoacylation
                     [Evidence IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="glutamate--tRNA ligase"
                     /protein_id="HOQ53861.1"
                     /translation="MTSSAPARLRVAPSPTGDPHVGTAYMSLFNKAYAEATGGAFVLR
                     IEDTDRVRYVAESEQQIFDTLRWLGFDWNEGPDVGGPYAPYRQSERLDTYRPFVEQLL
                     QSGHAYHCWCSTERLAQLRAQQQASKQSVTGYDRLCYGKTRDERAQLPGFSETPVVRM
                     LIPDDAPLTFTDVIRGEVSAPRPDDQVILKADGFPTYHLAVVVDDHLMGITHVVRGEE
                     WISSTPKHILLYQFLGLTPPAFAHMPLLRNPDKSKISKRKNPAARLMWFREQGYLPEA
                     LRNFLQLLAYPPVGESEVATFEQFVDAFDWAKVNTVGPIFDLKKLDWLNGEYIRAMDP
                     DDLADRIVEHLRFSGAWAEPDPAKVELLRRATPLIQERLGVLSEALGKLDFLFTADAD
                     LVIADDARAQLGADAPSVLAAAIEVLQTSEPFDTAGIEAGLRAALVDGLGLKPRFAFG
                     PVRVAITGSRVSPPLFESIELLGRESSVARVRALQKSLRGESS"
     gene            complement(2248..2526)
                     /locus_tag="PLF56_09635"
     CDS             complement(2248..2526)
                     /locus_tag="PLF56_09635"
                     /inference="COORDINATES: ab initio
                     prediction:GeneMarkS-2+"
                     /note="Derived by automated computational analysis using
                     gene prediction method: GeneMarkS-2+."
                     /codon_start=1
                     /transl_table=11
                     /product="hypothetical protein"
                     /protein_id="HOQ53862.1"
                     /translation="MSALLNARRSDASVSEGAYSLADLRTMILSDRTAAVAVRTVVSI
                     LIAVAAAIVIAGVATNFLIGTVGGGLSLLVFSATLLALNPDAAPSARG"
     gene            2725..>3876
                     /gene="glnS"
                     /locus_tag="PLF56_09640"
     CDS             2725..>3876
                     /gene="glnS"
                     /locus_tag="PLF56_09640"
                     /EC_number="6.1.1.18"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_016454992.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_component: GO:0005737 - cytoplasm [Evidence IEA];
                     GO_function: GO:0004819 - glutamine-tRNA ligase activity
                     [Evidence IEA];
                     GO_process: GO:0006425 - glutaminyl-tRNA aminoacylation
                     [Evidence IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="glutamine--tRNA ligase"
                     /protein_id="HOQ53863.1"
                     /translation="MTDAPAANDFIRDIVRRDNEQGTYGGRVQTRFPPEPNGYLHIGH
                     AKAIVVDFGIARDFGGVCNLRLDDTNPDTEDTEYVDSIVSDIAWLGYAPASVLYASDY
                     FDQLYAWAELLVQAGKAYVDDSDPETISASRGGFGKPGIDSAYRNRSVEENLDLLRRM
                     RAGEFADGARVLRAKIDMQHENMQMRDPVMYRIRHAHHHRTGDAWCIYPTYDWAHGQS
                     DAIEGVTHSICTLEFDTHRPLYDWYLEQLPLPSDQPKQIEFARLEFTHTVTSKRRLKK
                     LVDEGLVDGWDDPRMPTLRALRRRGYPAAAIRAFCRDIGTTRTNSRQAIEALESYVRR
                     DLNAVAQRRMAVLKPLKLVLTNWPEGQVESFEVANNPENPADGTRTVAFS"
BASE COUNT          668 a         1342 c         1293 g          573 t
ORIGIN      
        1 tgctcggggg tggacgagaa cggtgccggg ctcatgcccg cggtcatccg tccgatcacg
       61 aaaccgggac gcgcgatgat caggtcgacg ccggtgccgt gcagggcatc cgccatgccg
      121 gacgcgaaac cgtccagccc ggccttggcg gagccgtaga cgtagttggc ccggcgcacc
      181 cgctgcccgg caacggacga gaacgcgacg atcgtgccgg tgccgcgcgg cctcatcagg
      241 ccggccagca cggtcagcag ggacgcctgc gcggtgaagt cggtgtggat gatgcggacc
      301 acctcgccgg ggtcggtctc ggccctggcc tggtcgccca ggatgccgaa ggtcagcacg
      361 gcgatgtcga tcggacccac ctcgctctcg atcgctgcca ccgtggcggc gtgggcggcc
      421 acgtcgtcgg cgtcgaagtc gacgtgcagc acacgctcgg cacccggaac ctcaggacgg
      481 tcgccgccgg cccgggacgc cagcaccacc gtgttgccct cggccagtcg gcgcgcgatc
      541 tcgaggccga tctcggagcg gccgccgaag acggcaacca cgctcatggt gcggacccgc
      601 tcacgacgac tcgcccctca aggacttctg cagcgcccgc acccgtgcca cgctcgactc
      661 gcggccgagc aactcgatgg actcgaacag gggcggcgag acccgtgacc ccgtgatggc
      721 cacccgcacc gggccgaacg cgaagcgcgg tttgagcccc aacccgtcga ccagcgcggc
      781 ccgaagaccc gcttcgatac ccgcggtgtc gaacggctcg gacgtctgca gaacctcgat
      841 cgcggcggcc agcacggacg gcgcgtcggc gcccagctgg gcgcgcgcat cgtccgcgat
      901 caccaggtcg gcatcggcgg tgaacagaaa gtccagcttg cccagcgcct ccgacagcac
      961 gccgagccgc tcctggatca gcggcgtggc gcgccgcagc agttcgacct tcgccgggtc
     1021 tggctcggcc catgcgccgc tgaaccgcag gtgctcgacg atgcggtcgg cgagatcgtc
     1081 cggatccatc gcgcggatgt actcgccgtt cagccagtcc aacttcttca gatcgaagat
     1141 cggcccgacc gtgttcacct tggcccagtc gaaggcgtcg acgaactgct cgaaggtggc
     1201 cacctcggac tcaccgaccg gcggataggc cagcagctgc agaaagttgc gcaacgcctc
     1261 gggcaggtag ccctgctcgc ggaaccacat cagtcgcgcc gccgggttct tgcgcttgct
     1321 gatcttcgac ttgtcggggt tgcgcagcag tggcatgtgg gcgaaggccg gcggggtcag
     1381 gcccaggaac tggtacagca gaatgtgctt gggcgtggag ctgatccact cctcgccgcg
     1441 caccacatgg gtgatgccca tcaggtgatc gtccacgacc acggcgagat ggtaggtcgg
     1501 aaagccgtcc gccttgagaa tcacctgatc gtccggacgg ggggcgctca cctcgccacg
     1561 gatcacgtcg gtgaacgtga gcggtgcgtc gtccgggatc agcatgcgca ccaccggggt
     1621 ctcgctgaaa ccgggcagtt gtgcgcgctc gtcgcgggtc ttgccgtagc agagccggtc
     1681 gtagccggtc accgactgct tggacgcctg ctgctgcgcc cgcaactgcg ccaggcgttc
     1741 ggtggagcac cagcagtggt aggcgtgacc ggactgcagc aactgctcga cgaagggacg
     1801 gtaggtgtcc agccgctccg actgccggta gggggcgtac gggccaccca cgtcagggcc
     1861 ttcgttccag tcgaagccca gccagcgcag ggtgtcgaag atctgctgtt ccgactcggc
     1921 cacatagcgg acgcggtcgg tgtcttcgat gcgcagcacg aaggcaccgc cggtcgcttc
     1981 ggcgtaggcc ttgttgaaaa gcgacatgta ggcggtgccg acatgtgggt cgccggttgg
     2041 tgagggcgcg acccgaagtc gcgcgggagc tgaactggtc atcgcttgca gcctaccgaa
     2101 gggctgctgg aggcgtttcc cagccgggat cggcaacggt tctcaactcg gttgagaagc
     2161 gtccacagca cttgtccgag ggcggttctc cactcggttc ggggacccct ccgccgagca
     2221 acgagacgca cggaccgtag ctggccctca gccgcgggcc gagggggcgg cgtccgggtt
     2281 cagtgcgagc agagtggcgg agaagacgag caggctcaga cccccaccaa cggtgccgat
     2341 caagaagttg gtggcgacgc ccgcaatcac gatggcggcg gccactgcga tcaggatgct
     2401 cacaacggtg cggacggcca cggctgcggt gcggtcggac agaatcatgg tgcgaaggtc
     2461 ggccaggctg tacgccccct cggacacgga ggcgtcggac cggcgggcgt tgagcagtgc
     2521 ggacatgaga accgggttct ctctttctta tgtggagacg gcagtcccga acgggacagt
     2581 ccctatatta gtctgcttga ctaatataag caaaggcggc tccctcctgc ttccccccag
     2641 gccaccccac cgcgaccgct gtgaacggtc tcggcacacc ggcggcgctg agcgtcacac
     2701 accggcgttg gtaggtttgc cgtcatgacc gacgcgcccg ccgccaacga cttcatccgc
     2761 gacatcgtcc ggcgcgacaa cgagcagggc acctacggcg gacgcgtcca gacccgcttt
     2821 cctcccgagc ccaacgggta cctgcacatc ggccatgcca aggcgatcgt ggtcgatttc
     2881 ggcatcgcgc gcgacttcgg gggcgtgtgc aacctgcggc tggacgacac caatcccgac
     2941 accgaagaca ccgagtacgt cgactcgatc gtcagcgaca tcgcgtggct gggctacgcg
     3001 ccggcgtccg tgctgtacgc ctccgactac ttcgaccagc tctacgcgtg ggccgaactg
     3061 ctggtgcagg cgggcaaggc ctacgtcgac gactccgacc ccgagaccat ctcggcttct
     3121 cgcggcggct tcggcaagcc cggcatcgac agcgcctacc gcaaccgaag cgtcgaagag
     3181 aacctcgacc tgctgcgccg catgcgggcc ggcgagttcg ccgacggtgc ccgcgtgctg
     3241 cgggccaaga tcgacatgca gcacgagaac atgcagatgc gcgacccggt gatgtaccgc
     3301 atccggcacg cccaccatca ccgcaccggc gacgcctggt gcatctaccc gacctacgac
     3361 tgggcgcacg gccaatccga tgcgatcgag ggcgtcaccc actcgatctg cacgctcgag
     3421 ttcgacaccc accggccgct ctacgactgg tacctggagc agttgccgct gccgtccgac
     3481 caacccaagc agattgagtt cgcgcggctc gagttcaccc acacggtgac cagcaagcga
     3541 cggctcaaga agctcgtgga cgagggcctg gtcgacggct gggacgaccc ccgcatgccc
     3601 accctgcggg ccctacgccg gcgtgggtac ccggcggcgg cgatccgggc cttctgccgg
     3661 gacatcggca ccacccgcac caacagccgc caggcgatcg aggcgctgga gagctacgtc
     3721 cggcgcgacc tgaacgccgt cgcccagcgc cgcatggccg tgctgaaacc gctcaagctg
     3781 gtgctgacca actggcccga aggccaggtc gagtccttcg aggtggccaa caaccccgag
     3841 aaccctgcgg acggcacccg caccgtcgcc ttctcg
//
LOCUS       DAOSUG010000288         3867 bp    DNA     linear   ENV 10-JUN-2024
DEFINITION  MAG TPA_asm: Micropruina sp. isolate AMR_MDS_2195
            NODE_9781_length_3867_cov_3.958290, whole genome shotgun sequence.
ACCESSION   DAOSUG010000288 DAOSUG010000000
VERSION     DAOSUG010000288.1
DBLINK      BioProject: PRJNA925477
            BioSample: SAMN32864262
            Sequence Read Archive: SRR4236673
KEYWORDS    WGS; ENV; Metagenome Assembled Genome; MAG; Third Party Data; TPA;
            TPA:assembly.
SOURCE      Micropruina sp. (wastewater metagenome)
  ORGANISM  Micropruina sp.
            Bacteria; Bacillati; Actinomycetota; Actinomycetes;
            Propionibacteriales; Nocardioidaceae; Micropruina.
REFERENCE   1  (bases 1 to 3867)
  AUTHORS   Abdulkadir,N., Saraiva,J.P., Zhang,J., Stolte,S., Gillor,O.,
            Harms,H. and Rocha,U.
  TITLE     Genome-centric analyses of 165 metagenomes show that mobile genetic
            elements are crucial for the transmission of antimicrobial
            resistance genes to pathogens in activated sludge and wastewater
  JOURNAL   Microbiol Spectr 12 (3), e0291823 (2024)
   PUBMED   38289113
REFERENCE   2  (bases 1 to 3867)
  AUTHORS   Abdulkadir,N.
  TITLE     Direct Submission
  JOURNAL   Submitted (24-MAR-2023) Environmental Microbiology, Helmholtz
            Center for Environmental Research -UFZ, Permoserstr 15, Leipzig
            04318, Germany
COMMENT     The annotation was added by the NCBI Prokaryotic Genome Annotation
            Pipeline (PGAP). Information about PGAP can be found here:
            https://www.ncbi.nlm.nih.gov/genome/annotation_prok/
            
            ##Genome-Assembly-Data-START##
            Assembly Method        :: SPAdes v. 3.11.1
            Genome Representation  :: Full
            Expected Final Version :: Yes
            Genome Coverage        :: Not Applicable
            Sequencing Technology  :: Illumina MiSeq
            ##Genome-Assembly-Data-END##
            
            ##Genome-Annotation-Data-START##
            Annotation Provider               :: NCBI
            Annotation Date                   :: 05/04/2023 04:57:10
            Annotation Pipeline               :: NCBI Prokaryotic Genome
                                                 Annotation Pipeline (PGAP)
            Annotation Method                 :: Best-placed reference protein
                                                 set; GeneMarkS-2+
            Annotation Software revision      :: 6.5
            Features Annotated                :: Gene; CDS; rRNA; tRNA; ncRNA
            Genes (total)                     :: 2,776
            CDSs (total)                      :: 2,750
            Genes (coding)                    :: 2,714
            CDSs (with protein)               :: 2,714
            Genes (RNA)                       :: 26
            tRNAs                             :: 24
            ncRNAs                            :: 2
            Pseudo Genes (total)              :: 36
            CDSs (without protein)            :: 36
            Pseudo Genes (ambiguous residues) :: 0 of 36
            Pseudo Genes (frameshifted)       :: 2 of 36
            Pseudo Genes (incomplete)         :: 32 of 36
            Pseudo Genes (internal stop)      :: 2 of 36
            ##Genome-Annotation-Data-END##
FEATURES             Location/Qualifiers
     source          1..3867
                     /organism="Micropruina sp."
                     /mol_type="genomic DNA"
                     /submitter_seqid="NODE_9781_length_3867_cov_3.958290"
                     /isolate="AMR_MDS_2195"
                     /isolation_source="Activated sludge of municipal
                     wastewater treatment plants"
                     /db_xref="taxon:2737536"
                     /environmental_sample
                     /geo_loc_name="Canada"
                     /lat_lon="49.25 N 123.1 W"
                     /collection_date="2015-06-16"
                     /metagenome_source="wastewater metagenome"
                     /note="metagenomic"
     gene            complement(<1..1170)
                     /locus_tag="PLF56_09645"
     CDS             complement(<1..1170)
                     /locus_tag="PLF56_09645"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_013865137.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="CBS domain-containing protein"
                     /protein_id="HOQ53864.1"
                     /translation="MSSTASIFVSRIKGLPMLDASGDQVGKVRDVVLQNRPGNRAPRV
                     KGLVVELFALRRIFFPMERVHSIDVNQVIISGVVNTRRFEPRELESLAIDDLFDRRVF
                     RRGHNEPFIIYDLAIKQVRQRDWELSEVALRDVARRRPFAKPHVRIVPWNEVPDFTLI
                     PGRGADQVLAELQEMKPADVARELHDMDPQRRAEVVKAMDDELLAEALEELPEDEQVA
                     VIQSLDTERAADVLEEMDPDDAADLIAELAPEVAENILGRMEPEEAEDVRSLLTYDAS
                     TAGGLMTPEPVILAPDATVADALAMMRNEELTPALACLAFVCRTPLDTPTGRFLGAVH
                     IQRLLREPPSTLAASLVDKGIRPLPPAANIALVSRYFATYDLVCAPVVDVEGRLLG"
     gene            complement(1260..1673)
                     /locus_tag="PLF56_09650"
     CDS             complement(1260..1673)
                     /locus_tag="PLF56_09650"
                     /inference="COORDINATES: protein motif:HMM:NF002377.0"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_process: GO:0015031 - protein transport [Evidence IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="sec-independent translocase"
                     /protein_id="HOQ53865.1"
                     /translation="MDFNAGEVVLLLVLAIVLFGPEKLPELARKTARLLHHFRRIAND
                     AQGRLRQELGPEFADLELSDLNPKAFVAKHLGPDAANDLTDVATELKGARPSIDTALH
                     ATGTRTADDDDREGDQVEVPAAATQRDTPFDPEST"
     gene            complement(1755..2636)
                     /gene="dapA"
                     /locus_tag="PLF56_09655"
     CDS             complement(1755..2636)
                     /gene="dapA"
                     /locus_tag="PLF56_09655"
                     /EC_number="4.3.3.7"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_015069906.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_function: GO:0008840 - 4-hydroxy-tetrahydrodipicolinate
                     synthase activity [Evidence IEA];
                     GO_process: GO:0009089 - lysine biosynthetic process via
                     diaminopimelate [Evidence IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="4-hydroxy-tetrahydrodipicolinate synthase"
                     /protein_id="HOQ53866.1"
                     /translation="MSQPVFGRLLTAMVTPFDADGAIDLTQARKLAAHLVDVQRNDSL
                     VINGTTGESPTTTDAEKAELLAAVVAEVGDRAHVVAGVGTNDTRHTIELAHQAEQAGA
                     DGLLVVTPYYSRPPQDALAAHFTAVADATGLPVMLYDIPHRSGVPIEAATLIELAGHP
                     RIVAVKDAKGQVVSSATVIAATDLAYYAGDDGFTLPLLSVGGVGVVGTSTHFTGARAK
                     AMIDTFLAGDLAGAIAQNAALLPVFTGVFATQGCILVKAGLAARGFGSAVLRQPMLPA
                     SDAQASAFVALLDAAGL"
     gene            2769..3338
                     /locus_tag="PLF56_09660"
     CDS             2769..3338
                     /locus_tag="PLF56_09660"
                     /EC_number="2.1.1.-"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_015011426.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="class I SAM-dependent methyltransferase"
                     /protein_id="HOQ53867.1"
                     /translation="MRLARAASRQLALGAASQGVTSALTFLASLLHARAVVEIGTGAG
                     VSGLALIRGMAPDGILTSIDLEHEHQVAARQIFAAEGVSTRRVRLIAGAALTVLPKLS
                     DGAYDLVVVDGDPLEFVEYVAQAQRLLRSGGVLVVHHALWKGQVADESNDDDETLIIR
                     ESLQAVQEMDDFTATLLPVGDGLLVAVKA"
     gene            complement(3508..>3867)
                     /locus_tag="PLF56_09665"
     CDS             complement(3508..>3867)
                     /locus_tag="PLF56_09665"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_014846240.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="glucose-1-phosphate adenylyltransferase"
                     /protein_id="HOQ53868.1"
                     /translation="KWTMFTDQVQAPGAKFTLRGNAEDSIVSAGCIISGGDVDRSVLS
                     PNVVVDKWAKVEEAVIYPGCRIGRNAVVRRAILDKNVHVPDGVEIGVDPEHDRARGFY
                     HEDGITVVPKNFPVPAP"
BASE COUNT          608 a         1316 c         1329 g          614 t
ORIGIN      
        1 accgagcagg cgcccctcca cgtcgaccac gggcgcgcag accaggtcgt aggtggcgaa
       61 gtaacgcgag accagggcga tgttggccgc cggcggcagg ggtcgaatcc ccttgtcgac
      121 cagcgaagca gccagcgtgg agggcggctc gcgcaacagt cgctgaatgt gcaccgcgcc
      181 caggaaccgt ccggtcgggg tgtcgagcgg ggtgcggcag acgaaggcca gacacgccag
      241 cgcgggcgtg agctcttcgt tgcgcatcat cgccagcgcg tccgccacgg tcgcgtccgg
      301 ggccaggatc accggctcgg gcgtcatcag gccaccggcg gtcgacgcat cgtaggtgag
      361 cagcgagcgg acgtcctcgg cctcttcggg ctccattcgc cccagaatgt tctcggccac
      421 ctcgggggca agttcggcga tcaggtcggc ggcgtcgtcg gggtccatct cctcgagcac
      481 gtcggcggcg cgctcggtgt cgagcgactg gatcacggcg acctgttcgt cttcgggcag
      541 ctcttcgagg gcctcggcca gcagctcgtc gtccatggcc ttgaccacct cggcgcggcg
      601 ctgcggatcc atgtcgtgca gctcgcgagc gacgtccgcg ggcttcatct cctgcagttc
      661 ggcgagcacc tgatccgctc cccgtccggg aatcagggtg aagtcgggca cctcgttcca
      721 cggcacgatc ctcacgtgcg gcttggcgaa cggacgccgc cgagccacgt cacgcagcgc
      781 cacctcggac agttcccagt cacgctgccg cacctgtttg atcgccaggt cgtagatgat
      841 gaacggctcg ttgtggccgc ggcggaacac tctgcggtcg aacaggtcgt cgatggccag
      901 gctctccaat tcgcgcggct cgaagcggcg ggtgttgacc acgcccgaga tgatcacctg
      961 gttgacgtcg atggaatgca cccgctccat cggaaagaag atgcggcgca acgcgaaaag
     1021 ctcgaccacc aggcccttca cccgcggtgc acgattgccg ggacggttct gcagcacgac
     1081 atcgcgcacc ttgccgacct ggtcgccgct tgcgtccagc atcggcaggc ccttgatgcg
     1141 cgagacgaag atcgacgcgg tggagctcac aaccggaacg gtagcgcgtc gctgcccggg
     1201 ccgcgcggta ggaacggctg gcgatccgat cgccggccac cagtgctagg gcgcctctcc
     1261 tacgtcgact cgggatcgaa cggtgtgtcg cgctgcgtgg cggccgccgg cacctcgacc
     1321 tggtcaccct ctcgatcgtc gtcgtcggcg gttcgggtgc ctgtcgcgtg cagcgcggtg
     1381 tcgatgctgg ggcgggcgcc cttcaactcc gtggccacgt cggtcaggtc gttggcggca
     1441 tcggggccca ggtgtttggc gacgaaggcc ttcgggttca ggtccgacag ttcgaggtcg
     1501 gcgaactccg ggccgagctc ctgccgcagc ctgccctgcg cgtcgttggc gatgcgtcga
     1561 aagtgatgca gcaggcgggc ggtcttgcgg gccagctcgg gcagcttctc gggcccgaac
     1621 aaaacgatgg ccaacacgag caggagcacc acctcgcccg cgttgaaatc cacggctcac
     1681 agaatagtgc gccgccgcga gtggttcgcc gcacgggccc cgaccagcgc gaccaacgct
     1741 gggacggcgt ttcgctacag cccggcggcg tccaacagag cgacgaaggc ggacgcctgc
     1801 gcgtccgagg cgggcagcat cggttgccgc agcaccgccg agccgaagcc gcgcgcggcc
     1861 aggccggctt tcacgaggat gcaaccctgc gtggcgaaca cgcccgtgaa cacgggcagc
     1921 agggccgcat tctgagcgat ggccccggcg aggtcgccgg ccaggaacgt atcgatcatc
     1981 gccttggcac gggcgccggt gaagtgcgtg gaagtaccca ccacccccac cccgcccacc
     2041 gacagcagcg gcaacgtgaa gccgtcgtca ccggcgtagt aggccaggtc ggtggccgcg
     2101 atcacggtgg cggacgacac cacctggccc ttggcgtcct tcaccgcgac gatgcgcgga
     2161 tggccggcca gctcgatcag cgttgcggcc tcgatcggca cacccgaacg gtgcggaatg
     2221 tcgtacagca tgaccggcag cccagtggcg tccgcaacgg cggtgaagtg ggcggccagt
     2281 gcgtcctgcg gcgggcgcga atagtagggg gtgaccacga gcaggccatc tgcgccggcc
     2341 tgctcggcct ggtgtgccag ttcgatggtg tggcgggtgt cgttggtgcc cacgccggcc
     2401 acgacgtggg cccgatcacc cacctcggcc acgacggcgg ccagcagttc ggccttctcc
     2461 gcatcagtgg tggtgggcga ctcgcccgtg gtgccgttga tcaccaacga atcgttgcgc
     2521 tggacgtcca ccaggtgggc ggccagcttg cgggcctgcg tcagatcgat cgctccgtca
     2581 gcgtcgaaag gtgtgaccat cgcggtcagc agacggccga acacaggctg ggacatggcg
     2641 gcagtgtatc gggccgcttt cggtagggtt ggactgtgac tcctgcatcg cgtacggccg
     2701 ccaaggcgac caccacccct tcgccctcgt gggcgttctc cgagtcgttc gtcaccgacg
     2761 acgacaccat gcggctggct cgcgccgcca gccgtcagtt ggcgttgggc gccgcaagcc
     2821 agggagtcac ctcggcgctc accttccttg cctcgctgct gcacgccagg gcggtggtcg
     2881 agatcggcac cggcgcgggc gtgtcgggcc tggcgctgat tcgcggtatg gcgcctgacg
     2941 gcattttgac cagcatcgac ctcgaacacg aacaccaggt ggccgcccgg cagatcttcg
     3001 ccgccgaagg cgtgtccacc cgacgggtgc ggctgatcgc gggcgcggca ctgaccgtgc
     3061 tgcccaagct gagcgacggc gcctacgacc tcgtggtggt ggacggtgac ccgctcgagt
     3121 tcgtcgaata cgtcgcgcag gcccagcggc tgctgcgcag cggcggcgtg ctggtggtgc
     3181 atcacgccct gtggaagggc caggtcgcgg acgagtccaa cgacgacgac gagacgctga
     3241 tcatccgtga gtcgctgcag gccgtgcaag agatggacga cttcaccgcg accctgctgc
     3301 cggtgggcga cggactgctg gtggccgtca aggcctgagc gctccccggc gcagcgagtg
     3361 tgctaccgct gatcgagctt gccgagaccc gcgaaaccca gggtcctctc gctgacgaga
     3421 cctagggtcc tctcgctgaa cgagcttgtc gaaacctccc actagaggtt tcgacacgct
     3481 caaccaacag atcgcgcctc gtccgaccta cggagccgga accggaaagt tcttcggcac
     3541 caccgtgatg ccgtcctcgt ggtagaagcc gcgcgcccgg tcgtgttcgg ggtcgacgcc
     3601 gatctcgacg ccgtcgggca cgtgcacgtt cttgtcgaga atcgcccggc gcaccacggc
     3661 gttgcgtccg atccggcacc ccggatagat gacggcctct tcgacctttg cccacttgtc
     3721 gaccaccacg ttgggcgaga gcaccgagcg gtccacgtca ccgcccgaga tgatgcagcc
     3781 ggcgctgacg atcgaatcct ccgcgttgcc gcgcagggtg aacttggcgc ccggcgcctg
     3841 cacctggtcg gtgaacatcg tccactt
//
LOCUS       DAOSUG010000289         3864 bp    DNA     linear   ENV 10-JUN-2024
DEFINITION  MAG TPA_asm: Micropruina sp. isolate AMR_MDS_2195
            NODE_9788_length_3864_cov_4.223418, whole genome shotgun sequence.
ACCESSION   DAOSUG010000289 DAOSUG010000000
VERSION     DAOSUG010000289.1
DBLINK      BioProject: PRJNA925477
            BioSample: SAMN32864262
            Sequence Read Archive: SRR4236673
KEYWORDS    WGS; ENV; Metagenome Assembled Genome; MAG; Third Party Data; TPA;
            TPA:assembly.
SOURCE      Micropruina sp. (wastewater metagenome)
  ORGANISM  Micropruina sp.
            Bacteria; Bacillati; Actinomycetota; Actinomycetes;
            Propionibacteriales; Nocardioidaceae; Micropruina.
REFERENCE   1  (bases 1 to 3864)
  AUTHORS   Abdulkadir,N., Saraiva,J.P., Zhang,J., Stolte,S., Gillor,O.,
            Harms,H. and Rocha,U.
  TITLE     Genome-centric analyses of 165 metagenomes show that mobile genetic
            elements are crucial for the transmission of antimicrobial
            resistance genes to pathogens in activated sludge and wastewater
  JOURNAL   Microbiol Spectr 12 (3), e0291823 (2024)
   PUBMED   38289113
REFERENCE   2  (bases 1 to 3864)
  AUTHORS   Abdulkadir,N.
  TITLE     Direct Submission
  JOURNAL   Submitted (24-MAR-2023) Environmental Microbiology, Helmholtz
            Center for Environmental Research -UFZ, Permoserstr 15, Leipzig
            04318, Germany
COMMENT     The annotation was added by the NCBI Prokaryotic Genome Annotation
            Pipeline (PGAP). Information about PGAP can be found here:
            https://www.ncbi.nlm.nih.gov/genome/annotation_prok/
            
            ##Genome-Assembly-Data-START##
            Assembly Method        :: SPAdes v. 3.11.1
            Genome Representation  :: Full
            Expected Final Version :: Yes
            Genome Coverage        :: Not Applicable
            Sequencing Technology  :: Illumina MiSeq
            ##Genome-Assembly-Data-END##
            
            ##Genome-Annotation-Data-START##
            Annotation Provider               :: NCBI
            Annotation Date                   :: 05/04/2023 04:57:10
            Annotation Pipeline               :: NCBI Prokaryotic Genome
                                                 Annotation Pipeline (PGAP)
            Annotation Method                 :: Best-placed reference protein
                                                 set; GeneMarkS-2+
            Annotation Software revision      :: 6.5
            Features Annotated                :: Gene; CDS; rRNA; tRNA; ncRNA
            Genes (total)                     :: 2,776
            CDSs (total)                      :: 2,750
            Genes (coding)                    :: 2,714
            CDSs (with protein)               :: 2,714
            Genes (RNA)                       :: 26
            tRNAs                             :: 24
            ncRNAs                            :: 2
            Pseudo Genes (total)              :: 36
            CDSs (without protein)            :: 36
            Pseudo Genes (ambiguous residues) :: 0 of 36
            Pseudo Genes (frameshifted)       :: 2 of 36
            Pseudo Genes (incomplete)         :: 32 of 36
            Pseudo Genes (internal stop)      :: 2 of 36
            ##Genome-Annotation-Data-END##
FEATURES             Location/Qualifiers
     source          1..3864
                     /organism="Micropruina sp."
                     /mol_type="genomic DNA"
                     /submitter_seqid="NODE_9788_length_3864_cov_4.223418"
                     /isolate="AMR_MDS_2195"
                     /isolation_source="Activated sludge of municipal
                     wastewater treatment plants"
                     /db_xref="taxon:2737536"
                     /environmental_sample
                     /geo_loc_name="Canada"
                     /lat_lon="49.25 N 123.1 W"
                     /collection_date="2015-06-16"
                     /metagenome_source="wastewater metagenome"
                     /note="metagenomic"
     gene            103..297
                     /locus_tag="PLF56_09670"
     CDS             103..297
                     /locus_tag="PLF56_09670"
                     /inference="COORDINATES: ab initio
                     prediction:GeneMarkS-2+"
                     /note="Derived by automated computational analysis using
                     gene prediction method: GeneMarkS-2+."
                     /codon_start=1
                     /transl_table=11
                     /product="hypothetical protein"
                     /protein_id="HOQ53869.1"
                     /translation="MFDNLAAQSKCVQGEGYEVPPTPSWQSFLEQARAKSIDWDPTSR
                     VPEQLRSSARKACIDQDEWW"
     gene            300..1331
                     /locus_tag="PLF56_09675"
     CDS             300..1331
                     /locus_tag="PLF56_09675"
                     /inference="COORDINATES: protein motif:HMM:NF013625.2"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="biotin/lipoyl-binding protein"
                     /protein_id="HOQ53870.1"
                     /translation="MRRAVLWGLAVLVCGGLIGWAVAVATRPPQQVAAEASYVLVAAQ
                     DGEVGQSVSTVVSATWPVRSVAANQADGTITRVLVGRGDRVQAGDVLYRVDERPVVVL
                     EGSVPTFRSMQTGVVGADVRQLSNFLVGKKFLGAKTSTYSNAVQQAVRAWQRSLGVKQ
                     TGQVERGDVVFVKSLPMRVALDSKFAVGAQVSVGAPSIQAFAGAPVFVAQYSDFQAKQ
                     VAEGMPVVVQWGSQAWTGSLGALKVAADGAVTASVDGEDGSALCGTQCSKVPLSGTAQ
                     LKGTTEVQKSVRGVVVPLAALITDADGSTAVIAADGQRLPVTVLARARGMAVVQGVQV
                     GTSVRVPGQ"
     gene            1328..1966
                     /locus_tag="PLF56_09680"
     CDS             1328..1966
                     /locus_tag="PLF56_09680"
                     /inference="COORDINATES: protein motif:HMM:NF012235.2"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_function: GO:0005524 - ATP binding [Evidence IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="ATP-binding cassette domain-containing protein"
                     /protein_id="HOQ53871.1"
                     /translation="MISFGCERLAFGYENLPSILDGVSLTAQAGELVAITGASGRGKS
                     TLLYLLGLMLSPRAGAVLLDGKAVSGLRDAQRSALRAQLLGFVFQDAALDQTRTVMDN
                     ITEVCLYGDLDRNKAQQRAQRLIDQLGVQVPAHRRPGQISGGQAQRIALCRALVHDPA
                     VLLADEPTGNLDDSSAEGVIDAMRRHAETGRAVIVVTHDPRLEQACDRSVPL"
     gene            1963..3063
                     /locus_tag="PLF56_09685"
     CDS             1963..3063
                     /locus_tag="PLF56_09685"
                     /inference="COORDINATES: ab initio
                     prediction:GeneMarkS-2+"
                     /note="Derived by automated computational analysis using
                     gene prediction method: GeneMarkS-2+.
                     GO_function: GO:0042626 - ATPase-coupled transmembrane
                     transporter activity [Evidence IEA];
                     GO_function: GO:0140359 - ABC-type transporter activity
                     [Evidence IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="ABC transporter permease"
                     /protein_id="HOQ53872.1"
                     /translation="MTNTPGWWRKVLREALAMVLSQRVASLLTLVLVGGMSATVLLTA
                     GRTAAAERAILSSIDDAGSRAVVVRAEPNAGLDMSVVGRLSRIEQVEWVGAFGAARDA
                     TNGNTGGDVSAAVRTLRATNLATLGIDHPAPGECYASPKALNLLGMPDTTGYLVSDGT
                     PVCGVSGRLITPAFLAGFEPLVVAPLAPDSDDHVAVVVVLARHPEQVAAVTAATTALI
                     APLDPGAVSVTASPDIARLRALVQGQLGGFGRSLTLTLMAVLAALVGALLFGLVMIRR
                     KDFGRRRALGATRSLIVTLVSASTLMISAVASLLGGGVALLTLSIGGEPQPGWEFVAA
                     TLILTTAAATLGAVPPSIVASRRDPLTELRVP"
     gene            complement(3088..3270)
                     /locus_tag="PLF56_09690"
     CDS             complement(3088..3270)
                     /locus_tag="PLF56_09690"
                     /inference="COORDINATES: ab initio
                     prediction:GeneMarkS-2+"
                     /note="Derived by automated computational analysis using
                     gene prediction method: GeneMarkS-2+."
                     /codon_start=1
                     /transl_table=11
                     /product="hypothetical protein"
                     /protein_id="HOQ53873.1"
                     /translation="MTCSEERMRRGLAELAGGVKPRVDSSDLAAEIVRSVRRRRAAIG
                     LLVAVAGVWLVVSFVI"
BASE COUNT          657 a         1277 c         1271 g          659 t
ORIGIN      
        1 cccatcagtg gtgatagctc tgccgggcag gttgcctgga gtgcgtgtcg ggatgagctt
       61 cccgcgcgtc ctgagcctac gagcgatgct gatttcaaag tcatgttcga caatttggcg
      121 gcgcagagta aatgtgtgca gggcgagggt tacgaggtgc cgcccacccc gtcgtggcag
      181 tccttcctgg aacaggcccg ggcgaaaagt atcgattggg atccgacgtc ccgggtgccg
      241 gaacagttgc gttcgtcggc gcgtaaggcg tgcatcgatc aggacgagtg gtggtgagcg
      301 tgcgccgggc tgtcctgtgg ggcctggctg tgttggtatg tggagggctg atcggttggg
      361 cggtcgcggt ggcgactcgt ccgccgcagc aggtggccgc tgaagccagt tatgtgttgg
      421 tggctgcaca ggacggcgaa gtcggccagt cggtttcaac agttgtttcg gcgacctggc
      481 cggttcgcag cgtggcagcc aatcaggctg acggcacgat cacccgagtg ttggtgggcc
      541 gcggtgatcg cgtgcaggcc ggggacgtgc tctaccgggt ggacgagcgt ccggtggtgg
      601 ttcttgaagg gtcggtgcca acgttccggt cgatgcaaac gggtgtcgtc ggcgccgatg
      661 tgcgtcagtt gagcaatttc ttggtgggca agaagttcct cggcgcgaag acgtcgacct
      721 actcgaatgc ggtgcaacag gccgtccgcg cctggcaacg gtcgttgggg gtcaagcaga
      781 ctggtcaggt tgaacgaggc gatgtggtgt tcgtgaaatc attgccgatg cgggtcgcgt
      841 tggattccaa gtttgcggtc ggtgcgcagg tgtcggtggg cgctccgtcg atccaggcct
      901 tcgccggcgc gccggttttc gttgctcagt acagcgattt tcaggccaag caggtggccg
      961 agggtatgcc ggtggttgtg cagtgggggt cgcaggcctg gaccgggtct ctgggtgcgt
     1021 tgaaggtggc cgctgacggt gcggtgacgg cgtccgttga tggcgaggac ggttccgctc
     1081 tgtgcggcac gcagtgcagc aaggtgccct tgagcggcac agcccagctg aagggcacca
     1141 ccgaggtgca gaaatccgta cggggtgtcg tcgtcccgtt ggctgcattg atcaccgacg
     1201 cggacggatc gacggctgtg atcgccgcgg acggtcagcg actgcccgtg accgtgctcg
     1261 cgcgtgcccg tggcatggcc gtggtccaag gggtgcaggt ggggacttcc gtgcgggtac
     1321 cgggtcaatg atctcgttcg gttgcgagcg gctcgcgttc ggctacgaga acctgcccag
     1381 catccttgat ggtgtctcac tcaccgcgca ggccggcgag ttggtggcca tcaccggagc
     1441 ttcaggacgc ggcaagtcga cacttctgta tctgctgggt ctgatgctgt cgccgcgggc
     1501 cggtgcggta ctgctcgacg gcaaggctgt gtcgggcctt cgagacgctc agcgctcggc
     1561 gctgcgcgcg cagttgctcg ggttcgtgtt tcaggacgcc gcacttgacc agacccgcac
     1621 ggtgatggac aacatcaccg aggtctgtct gtacggcgac cttgaccgga acaaggccca
     1681 acagagggcg caacggttga tcgaccagct tggcgtccag gtaccggcac accgccgacc
     1741 cgggcaaatc tccggcggcc aagcgcaacg catcgctctg tgtcgcgccc tggtgcacga
     1801 cccggcagtg ctacttgccg acgaaccgac cgggaatctc gacgacagca gcgccgaggg
     1861 cgtcatcgat gcgatgcgcc ggcacgctga aaccggccgg gccgtcatcg tggtcaccca
     1921 cgatccccga cttgagcaag cctgcgaccg gtcggtgccc ctatgaccaa cacacccggc
     1981 tggtggcgca aggtgcttcg ggaagccctg gcgatggtgc tgtcccagcg ggtggcgtcg
     2041 ctactcactc tcgtcctcgt cggcggcatg agcgccaccg tgctgctgac cgccggccga
     2101 accgctgccg ccgagcgggc catcctgtcc agcatcgacg acgccggatc acgcgccgtc
     2161 gtggtgcggg ccgaaccgaa cgccggactc gacatgagcg tggtcggccg actcagccgc
     2221 atcgagcagg tcgagtgggt cggagcgttc ggcgcggcac gcgacgccac caacggcaac
     2281 accggcggcg acgtctcggc cgccgtcaga acactacgcg caaccaacct tgccaccctg
     2341 ggcatcgatc accccgcccc aggcgagtgc tacgccagcc ccaaagccct caacctcctc
     2401 ggcatgcccg acaccaccgg ctacctcgtc agcgacggaa cccccgtctg cggcgtcagc
     2461 ggacgcctca tcacaccggc cttcctcgcc ggcttcgaac cgttggtcgt ggccccgctc
     2521 gccccggatt ccgacgacca cgtggctgtt gtggtcgtcc tggccaggca ccccgaacag
     2581 gtagcagctg tgacagcagc caccacagcc ctcatcgcac ccctcgaccc aggcgctgtc
     2641 agcgtcaccg cctcacccga catcgccaga ctccgcgcac tggtccaagg acaactcgga
     2701 ggattcggac gcagcctcac cctcaccctg atggccgtgc tggccgcgct cgtcggggct
     2761 ctcctgttcg ggctggtcat gatccggcgc aaggacttcg gacgccgacg tgccctgggc
     2821 gccacccgaa gcctcatcgt caccctcgtc agcgcgagca ctctgatgat ctctgccgtg
     2881 gccagcctgc tcggcggcgg cgtcgcccta ctgaccctga gcatcggcgg agaaccccaa
     2941 cccggctggg agttcgtcgc cgccaccctg atcctgacca cagccgcagc cacactagga
     3001 gccgtgccac ccagcatcgt cgcatcccga cgagacccac tcaccgaact acgagtgccc
     3061 tgattcgcac ccgaagtgag tgccgcacta gatgacgaaa ctcacgacca gccacacccc
     3121 cgccaccgcc accaacaggc cgatcgctgc ccgacgccgg cgcaccgatc gcacgatctc
     3181 agcagcaaga tccgagctgt ccacccgggg cttgacaccc ccggccagtt cagccaaccc
     3241 ccggcgcatg cgctcctccg aacaggtcat cgcactacca cccctccctt gatcgaactc
     3301 agtcatcaac cccatacgcc acccgcaccc aataggttgc ttgattcggt ctcgagcgcc
     3361 cgaccaacaa aaggaatcga caagatagtg agaatccgca accgatgagc aaactgtcgt
     3421 cgccctagtc agcccaagtt gcgaacgaca gaacgtgcga gaacctacag ctgcttcctt
     3481 cttcgcgttc gacgtcgagg ggcgctgctc accttgctta ggggctcgtg caaggacgcg
     3541 gactctgccg ggtcgtgggt tcatccgcac gagcggaagc cggtcgccag ggtaggttcg
     3601 attccgccgg catggccggc gtcggcacgg caacggccgc gatcgtcgcc gcaaatcgag
     3661 agtggaaggg gtgacgcgac ctgtcaacgg agcgtcccac ggggcagggc gacgactggc
     3721 agtcggacac cggaaccgac agcggctggt tcttcagcga gaccagcgac gatgacgcgg
     3781 gggtgaccta ccgcggcgag cgagacgcat cgtcaggcaa ctcatcacca acggcctcgc
     3841 cgagcagcga ctcgcatgtc ggcc
//
LOCUS       DAOSUG010000290         3849 bp    DNA     linear   ENV 10-JUN-2024
DEFINITION  MAG TPA_asm: Micropruina sp. isolate AMR_MDS_2195
            NODE_9830_length_3849_cov_5.287559, whole genome shotgun sequence.
ACCESSION   DAOSUG010000290 DAOSUG010000000
VERSION     DAOSUG010000290.1
DBLINK      BioProject: PRJNA925477
            BioSample: SAMN32864262
            Sequence Read Archive: SRR4236673
KEYWORDS    WGS; ENV; Metagenome Assembled Genome; MAG; Third Party Data; TPA;
            TPA:assembly.
SOURCE      Micropruina sp. (wastewater metagenome)
  ORGANISM  Micropruina sp.
            Bacteria; Bacillati; Actinomycetota; Actinomycetes;
            Propionibacteriales; Nocardioidaceae; Micropruina.
REFERENCE   1  (bases 1 to 3849)
  AUTHORS   Abdulkadir,N., Saraiva,J.P., Zhang,J., Stolte,S., Gillor,O.,
            Harms,H. and Rocha,U.
  TITLE     Genome-centric analyses of 165 metagenomes show that mobile genetic
            elements are crucial for the transmission of antimicrobial
            resistance genes to pathogens in activated sludge and wastewater
  JOURNAL   Microbiol Spectr 12 (3), e0291823 (2024)
   PUBMED   38289113
REFERENCE   2  (bases 1 to 3849)
  AUTHORS   Abdulkadir,N.
  TITLE     Direct Submission
  JOURNAL   Submitted (24-MAR-2023) Environmental Microbiology, Helmholtz
            Center for Environmental Research -UFZ, Permoserstr 15, Leipzig
            04318, Germany
COMMENT     The annotation was added by the NCBI Prokaryotic Genome Annotation
            Pipeline (PGAP). Information about PGAP can be found here:
            https://www.ncbi.nlm.nih.gov/genome/annotation_prok/
            
            ##Genome-Assembly-Data-START##
            Assembly Method        :: SPAdes v. 3.11.1
            Genome Representation  :: Full
            Expected Final Version :: Yes
            Genome Coverage        :: Not Applicable
            Sequencing Technology  :: Illumina MiSeq
            ##Genome-Assembly-Data-END##
            
            ##Genome-Annotation-Data-START##
            Annotation Provider               :: NCBI
            Annotation Date                   :: 05/04/2023 04:57:10
            Annotation Pipeline               :: NCBI Prokaryotic Genome
                                                 Annotation Pipeline (PGAP)
            Annotation Method                 :: Best-placed reference protein
                                                 set; GeneMarkS-2+
            Annotation Software revision      :: 6.5
            Features Annotated                :: Gene; CDS; rRNA; tRNA; ncRNA
            Genes (total)                     :: 2,776
            CDSs (total)                      :: 2,750
            Genes (coding)                    :: 2,714
            CDSs (with protein)               :: 2,714
            Genes (RNA)                       :: 26
            tRNAs                             :: 24
            ncRNAs                            :: 2
            Pseudo Genes (total)              :: 36
            CDSs (without protein)            :: 36
            Pseudo Genes (ambiguous residues) :: 0 of 36
            Pseudo Genes (frameshifted)       :: 2 of 36
            Pseudo Genes (incomplete)         :: 32 of 36
            Pseudo Genes (internal stop)      :: 2 of 36
            ##Genome-Annotation-Data-END##
FEATURES             Location/Qualifiers
     source          1..3849
                     /organism="Micropruina sp."
                     /mol_type="genomic DNA"
                     /submitter_seqid="NODE_9830_length_3849_cov_5.287559"
                     /isolate="AMR_MDS_2195"
                     /isolation_source="Activated sludge of municipal
                     wastewater treatment plants"
                     /db_xref="taxon:2737536"
                     /environmental_sample
                     /geo_loc_name="Canada"
                     /lat_lon="49.25 N 123.1 W"
                     /collection_date="2015-06-16"
                     /metagenome_source="wastewater metagenome"
                     /note="metagenomic"
     gene            complement(<1..613)
                     /locus_tag="PLF56_09695"
     CDS             complement(<1..613)
                     /locus_tag="PLF56_09695"
                     /inference="COORDINATES: ab initio
                     prediction:GeneMarkS-2+"
                     /note="Derived by automated computational analysis using
                     gene prediction method: GeneMarkS-2+."
                     /codon_start=1
                     /transl_table=11
                     /product="hypothetical protein"
                     /protein_id="HOQ53874.1"
                     /translation="MSRAKRFWVALGAVLVVCAVVAAILAGRPSREPSDTSVDAAPPA
                     SGSAGLGDPYYPDAGGGGYDVQHYDVRVRADGPRSEVRGTTTVTAIATQDLDRLHLDL
                     LLSARSATVNGHPATIEQHGDDLSVLAPRADPATPAIRGGSTFTVTIDYAGLPGKVQR
                     GREPAYYQSGDEFVIAGEPLGASLWYPANDHPRDAATMTFTVSV"
     gene            complement(723..1328)
                     /locus_tag="PLF56_09700"
     CDS             complement(723..1328)
                     /locus_tag="PLF56_09700"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_011756042.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_function: GO:0016491 - oxidoreductase activity
                     [Evidence IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="NAD(P)H-dependent oxidoreductase"
                     /protein_id="HOQ53875.1"
                     /translation="MTDTVVVLTAGVGTPSSSQLLGEQLAKATQRVLAAAGKPVELVS
                     IELRTLASDLAHQLTTHVPSPALTQVYGAVGRAVGVIVVTPVFNASYSGLFKLFFDLM
                     DEGVMAARPVLLAATGGTARHSLVIDTAMLPLFHYLKAAIAPVGVFAATGDWGDQAGQ
                     LKARIESAARAFGQLVAERPGVTVEDEFAVGIDFENLLHGA"
     gene            complement(1325..2488)
                     /locus_tag="PLF56_09705"
     CDS             complement(1325..2488)
                     /locus_tag="PLF56_09705"
                     /EC_number="1.-.-.-"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_017935296.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_function: GO:0010181 - FMN binding [Evidence IEA];
                     GO_function: GO:0016491 - oxidoreductase activity
                     [Evidence IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="LLM class flavin-dependent oxidoreductase"
                     /protein_id="HOQ53876.1"
                     /translation="MQFGIFTVGDVTTDPTTGVTPTEHERIKAAVAIATKAEEVGLDV
                     FAHGEHHNPPFHPSSPTTLLGYVAAKTERILLSTATTLITTNDPVKIAEDYSVLQHLA
                     DGRVDLMLGRGNTGPVYPWFGRDIRDGISLAVENYALLRRLWREPVVDWEGKFRTPLQ
                     GFTLAPQPLDGVPPFVWHGSIRSPQIAEQAAFYGDGFFHNHIFWPQTHTRQMVELYRR
                     RWEHYGHGPANTAIVGLGGQFFMRRNSQDAVREFRPYFDVAPVYGHGPSLEEFMAQTP
                     LTVGSPQQVLERTLGFREVVGDYQRQLFLIDHAGLPLKTVLEQLDLFGEILPELRKGF
                     AKDRPAEVPDAPTHASLVAAAEAERTVSTALPTAVDDVTGRSPYQAVEAGAKL"
     gene            2831..3610
                     /locus_tag="PLF56_09710"
     CDS             2831..3610
                     /locus_tag="PLF56_09710"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_012920397.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="Sir2 family NAD-dependent protein deacetylase"
                     /protein_id="HOQ53877.1"
                     /translation="MDDPANARALLRQAGRVAVLTGAGISTASGIGDFRGPQGRWTLD
                     PQAERVSTLSWYLNDPDVRANAWRFRAESPVYNAQPNAAHRALVRLEQGARLTGLITQ
                     NTDGLHQLAGSNPELVIELHGNSRTWRCESCRATGPMIDMVRLVRAGALDPRCPYDGG
                     IVRATTILFEEPLDPAVIDEAVASVDDADALLVIGTSLGVHPAAGLVPYAHRRGVQIV
                     IVNAEPTDYDGLAAFVLRDPIVGIVPVLLGSDDEQISTIAE"
BASE COUNT          595 a         1347 c         1328 g          579 t
ORIGIN      
        1 gcacgctgac ggtgaacgtc atcgtggcgg cgtcgcgtgg gtgatcgttg gccgggtacc
       61 acagcgaggc gccgagcggc tcacccgcga tcacgaactc gtcgccgctt tggtagtagg
      121 cgggctcgcg gccgcgctgc accttgccgg gcaggcccgc atagtcgatg gtcaccgtga
      181 acgtggagcc gccgcggata gccggggtgg cagggtcggc gcgtggggcc agcacgctca
      241 ggtcatcgcc atgctgctcg atggtggccg ggtgcccgtt cacggtggcc gatcgcgcac
      301 tcagcagtag gtcgaggtgg aggcggtcaa ggtcctgcgt cgcgatcgcc gtcaccgtgg
      361 tcgtgccgcg cacctcactg cgcggcccgt ccgcgcgcac ccgcacgtcg tagtgctgga
      421 cgtcgtaccc gccaccacct gcgtccgggt agtaggggtc acccaatccg gcgctgccgc
      481 tggccggggg cgccgcgtcc accgaagtgt cggacggctc gcggctcgga cggccggcca
      541 gtatcgccgc caccacagcg cacaccacca gaacagcgcc cagcgcgacc cagaaccgtt
      601 tcgctcgcga cacccccata gactgccagg cccggccaag acccgccgag cgcctcccta
      661 accatctctg gcccgcgaag gttcgcgggc cagagatggt taggggggcg ctcggcgggt
      721 gttcaggccc cgtgcagcag gttctcgaag tcgatgccga cggcgaactc gtcctcgacg
      781 gtcacccccg gacgctcggc caccagctgc ccgaaggccc gggccgccga ctcgatccgt
      841 gccttcagct gccccgcctg atcaccccag tcacccgtgg ccgcgaacac cccgaccggg
      901 gcgatggcgg ccttcaggta atggaacagc ggcagcatcg cggtgtcgat caccagggaa
      961 tgccgcgcgg taccaccggt cgcggccagc agtaccggcc gcgcggccat cacgccctcg
     1021 tccatcaggt cgaagaacag cttgaacagg cccgagtacg acgcgttgaa caccggcgtc
     1081 acgacgatca ccccgaccgc ccggccgacc gcgccgtaca cctgcgtgag cgccggcgag
     1141 ggtacgtgcg tggtcagctg atgagccaga tcgctcgcca gcgtgcgcag ctcgatcgac
     1201 accagctcga ccggtttgcc ggccgcggcc agcacccgct gggtcgcctt ggccaactgc
     1261 tccccgagca actggctgga cgagggtgtg cccaccccgg ccgtgagcac caccacggtg
     1321 tcggtcataa cttggcaccc gcctcgacgg cctggtaggg actgcgtccg gtcacgtcgt
     1381 cgacggcggt cggcaacgcg gtcgagaccg tgcgctccgc ttcggccgct gcgaccagcg
     1441 aggcgtgcgt cggggcgtcc ggcacctcgg ccggacggtc cttcgcgaac cccttgcgca
     1501 gctcgggcag aatctcgccg aacaggtcga gttgctccag cacggtcttc agcggcagcc
     1561 ccgcatggtc gatcaggaac agctggcgct ggtagtcgcc gaccacctcg cggaagccga
     1621 gcgtgcgctc gagcacctgc tgcggcgatc cgacggtcag cggcgtctgc gccatgaact
     1681 cctccaggct cggcccgtgc ccgtacaccg gcgccacatc gaagtacgga cggaactcgc
     1741 gcacggcgtc ctggctgttg cggcgcatga agaactgccc gcccagcccg acgatcgcgg
     1801 tgttggccgg tccgtggccg tagtgctccc agcgccggcg gtacagctcg accatctgac
     1861 gggtgtgggt ctgcggccag aagatgtggt tgtgaaagaa gccgtcgccg tagaacgcgg
     1921 cctgctcggc gatctgcggg ctgcggatcg agccgtgcca gacgaacggc ggcaccccgt
     1981 ccagcggctg cggggcgagc gtgaagccct gcaggggggt gcggaacttg ccctcccagt
     2041 cgaccacggg ctcgcgccag agccggcgca gcagggcgta gttctcgacc gccaggctga
     2101 tgccgtcgcg aatgtcgcga ccgaaccagg gatacaccgg gccggtgttg ccgcggccga
     2161 gcatcaggtc gacccgtccg tcggccaggt gctgcagcac ggagtagtcc tcggcgatct
     2221 tgaccgggtc gttggtggtg atcagcgtgg tggccgtcga cagcagaatg cgttcggtct
     2281 tggccgcgac gtagccgagc agcgtggtgg ggctggacgg gtggaacggc gggttgtggt
     2341 gctcaccgtg cgcgaacacg tccaggccga cctcttcggc cttcgtggcg atggccacgg
     2401 cggccttgat gcgctcgtgc tcggtgggcg tgacaccggt ggtggggtcg gtggtgacgt
     2461 cgccgacggt gaagatgccg aactgcatga ctgctcccaa gtagttgtag tttcaactat
     2521 acccgtcaac gtacgcgtgg tcgagggtat tccgctgcac cggccgagca ccgcgcggtc
     2581 gagctggagg aggagacgcc tgccggtcga gaccccccac cgcccggaga accgtccccc
     2641 tctccgttca ggaccctcca gggctcgacg tcgcgcgctc gagcgttcgt tgcgcgcgga
     2701 cgaacccgcg caatgagccc tggaccgcgc gacgtcgtgg tagctgggtc tcgacacgct
     2761 cgaccaacgg aagtgggcct cgaccgacga aactctcgac ccggcgtcgg ccccttgcca
     2821 cactggcctg atggacgatc ccgcgaacgc ccgcgccctg ctccggcagg ccggccgggt
     2881 ggccgtgctc accggagccg gcatctcgac cgcctccggt atcggtgact ttcgcggtcc
     2941 gcaggggcga tggacgctcg acccgcaggc cgagcgggtc tcgacgctct cgtggtacct
     3001 caacgacccg gacgtccggg ccaacgcgtg gcgattccgg gccgagtccc ccgtgtacaa
     3061 cgcgcagccg aatgctgccc accgcgccct ggtgcggctc gagcagggcg cccggctgac
     3121 cgggctgatc acccagaaca ccgacggcct gcaccagctg gccggctcga accccgagtt
     3181 ggtgatcgag ctgcacggca acagccgcac ctggcgctgc gagagttgcc gggcgaccgg
     3241 gccgatgatc gacatggtcc gcctggtccg cgcgggcgcg ctcgatccgc gctgccccta
     3301 cgacggcggc atcgtccggg cgaccacgat cctgttcgag gaaccgctcg acccggcggt
     3361 catcgatgag gccgtcgcct cggtcgacga cgccgacgcg ctactggtga tcggcacctc
     3421 actgggcgtt cacccggcgg ccggcctggt gccgtacgca caccggcgcg gcgtccagat
     3481 cgtgatcgtg aacgccgaac cgaccgacta cgacggcctg gccgcgttcg tgctgcgcga
     3541 cccgatcgtg ggcatcgtcc ccgtcctact cgggtcagat gacgaacaga tatcgacaat
     3601 cgctgaatga gacctccggc gaagctctgg cgctgcggac gcattgccgc ttagatgcgt
     3661 tcacaacggg agcgggggaa gttccggtac gcaaggttgc agcatggtca caatccgtca
     3721 cggaagggcc atacggcttc gtttgtgcgc aggcgcccgc catagttgcc gcatcatcgc
     3781 gacggcgggt cgcgactgac aaggaggaaa tgtgcgtaac aagaagctgc tgggtggcgc
     3841 tgccgccct
//
LOCUS       DAOSUG010000291         3837 bp    DNA     linear   ENV 10-JUN-2024
DEFINITION  MAG TPA_asm: Micropruina sp. isolate AMR_MDS_2195
            NODE_9862_length_3837_cov_4.064516, whole genome shotgun sequence.
ACCESSION   DAOSUG010000291 DAOSUG010000000
VERSION     DAOSUG010000291.1
DBLINK      BioProject: PRJNA925477
            BioSample: SAMN32864262
            Sequence Read Archive: SRR4236673
KEYWORDS    WGS; ENV; Metagenome Assembled Genome; MAG; Third Party Data; TPA;
            TPA:assembly.
SOURCE      Micropruina sp. (wastewater metagenome)
  ORGANISM  Micropruina sp.
            Bacteria; Bacillati; Actinomycetota; Actinomycetes;
            Propionibacteriales; Nocardioidaceae; Micropruina.
REFERENCE   1  (bases 1 to 3837)
  AUTHORS   Abdulkadir,N., Saraiva,J.P., Zhang,J., Stolte,S., Gillor,O.,
            Harms,H. and Rocha,U.
  TITLE     Genome-centric analyses of 165 metagenomes show that mobile genetic
            elements are crucial for the transmission of antimicrobial
            resistance genes to pathogens in activated sludge and wastewater
  JOURNAL   Microbiol Spectr 12 (3), e0291823 (2024)
   PUBMED   38289113
REFERENCE   2  (bases 1 to 3837)
  AUTHORS   Abdulkadir,N.
  TITLE     Direct Submission
  JOURNAL   Submitted (24-MAR-2023) Environmental Microbiology, Helmholtz
            Center for Environmental Research -UFZ, Permoserstr 15, Leipzig
            04318, Germany
COMMENT     The annotation was added by the NCBI Prokaryotic Genome Annotation
            Pipeline (PGAP). Information about PGAP can be found here:
            https://www.ncbi.nlm.nih.gov/genome/annotation_prok/
            
            ##Genome-Assembly-Data-START##
            Assembly Method        :: SPAdes v. 3.11.1
            Genome Representation  :: Full
            Expected Final Version :: Yes
            Genome Coverage        :: Not Applicable
            Sequencing Technology  :: Illumina MiSeq
            ##Genome-Assembly-Data-END##
            
            ##Genome-Annotation-Data-START##
            Annotation Provider               :: NCBI
            Annotation Date                   :: 05/04/2023 04:57:10
            Annotation Pipeline               :: NCBI Prokaryotic Genome
                                                 Annotation Pipeline (PGAP)
            Annotation Method                 :: Best-placed reference protein
                                                 set; GeneMarkS-2+
            Annotation Software revision      :: 6.5
            Features Annotated                :: Gene; CDS; rRNA; tRNA; ncRNA
            Genes (total)                     :: 2,776
            CDSs (total)                      :: 2,750
            Genes (coding)                    :: 2,714
            CDSs (with protein)               :: 2,714
            Genes (RNA)                       :: 26
            tRNAs                             :: 24
            ncRNAs                            :: 2
            Pseudo Genes (total)              :: 36
            CDSs (without protein)            :: 36
            Pseudo Genes (ambiguous residues) :: 0 of 36
            Pseudo Genes (frameshifted)       :: 2 of 36
            Pseudo Genes (incomplete)         :: 32 of 36
            Pseudo Genes (internal stop)      :: 2 of 36
            ##Genome-Annotation-Data-END##
FEATURES             Location/Qualifiers
     source          1..3837
                     /organism="Micropruina sp."
                     /mol_type="genomic DNA"
                     /submitter_seqid="NODE_9862_length_3837_cov_4.064516"
                     /isolate="AMR_MDS_2195"
                     /isolation_source="Activated sludge of municipal
                     wastewater treatment plants"
                     /db_xref="taxon:2737536"
                     /environmental_sample
                     /geo_loc_name="Canada"
                     /lat_lon="49.25 N 123.1 W"
                     /collection_date="2015-06-16"
                     /metagenome_source="wastewater metagenome"
                     /note="metagenomic"
     gene            171..599
                     /locus_tag="PLF56_09715"
     CDS             171..599
                     /locus_tag="PLF56_09715"
                     /inference="COORDINATES: protein motif:HMM:TIGR03847.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="DUF3090 family protein"
                     /protein_id="HOQ53878.1"
                     /translation="MPTIVHEFAWPDRLVVGTVGRPGDREFYLQARTKARIVTIALEK
                     QQSAQLAEKIDEVLDELRSTEGNPFRVPDSMPVELVDNDPLDQPIDTQFRTGAMRLGW
                     DPMTAQIVIEAYAMTDADYDEDGPQPIKIEPPSHCWCTCR"
     gene            602..724
                     /locus_tag="PLF56_09720"
     CDS             602..724
                     /locus_tag="PLF56_09720"
                     /inference="COORDINATES: protein motif:HMM:NF022734.2"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="DUF3090 family protein"
                     /protein_id="HOQ53879.1"
                     /translation="MARAFAIRTLEVVGAGRPSCSLCGQPMNPEGHLCPQPGAL"
     gene            873..1421
                     /locus_tag="PLF56_09725"
     CDS             873..1421
                     /locus_tag="PLF56_09725"
                     /inference="COORDINATES: ab initio
                     prediction:GeneMarkS-2+"
                     /note="Derived by automated computational analysis using
                     gene prediction method: GeneMarkS-2+."
                     /codon_start=1
                     /transl_table=11
                     /product="hypothetical protein"
                     /protein_id="HOQ53880.1"
                     /translation="MRLFDKIRMTVDVLKSVRRPTVPLAAARARYNPMVHTLADALTA
                     EFGVTWTVAFEAELVPFGRRGDYTTQRLISTDRLDAFPEWSARYADVVQSVAQVTGFG
                     TQGYDPKESQQRSEPAPSERQTYVLDDDVGVVLEPIDIPDSGPSRITRDSLGGIIVLE
                     IDSDGTLIFIAAAVPADNHETH"
     gene            1491..1886
                     /locus_tag="PLF56_09730"
     CDS             1491..1886
                     /locus_tag="PLF56_09730"
                     /inference="COORDINATES: ab initio
                     prediction:GeneMarkS-2+"
                     /note="Derived by automated computational analysis using
                     gene prediction method: GeneMarkS-2+."
                     /codon_start=1
                     /transl_table=11
                     /product="hypothetical protein"
                     /protein_id="HOQ53881.1"
                     /translation="MTAEQPKPRVLIIGLDPHRVPGPWDPEPVAEAIRDGFTEFAKHG
                     VEAQACLFGLDGSDNPEDVIGTVLQSQPWECVVIGGGVRTDDNLLDLFERIINLVRRL
                     APGAAIAFNASPTETYAAASRWIDVGDDR"
     gene            complement(2025..2459)
                     /locus_tag="PLF56_09735"
     CDS             complement(2025..2459)
                     /locus_tag="PLF56_09735"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_008356778.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_function: GO:0003723 - RNA binding [Evidence IEA];
                     GO_function: GO:0008173 - RNA methyltransferase activity
                     [Evidence IEA];
                     GO_process: GO:0006396 - RNA processing [Evidence IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="TrmH family RNA methyltransferase"
                     /protein_id="HOQ53882.1"
                     /translation="MAPLWVAYGANLGTLQRTCDAVGACLAVPDTPHYREALSKGNTL
                     GHRRPCLHRVTAKEPWVRSMQEQGWRVVAVELAEGSVPLPRLQPARLPTIVLLGHEHH
                     GVPDDMVDLADECVEIPMCGVGASLNVAVAGSLVLYRLAGLS"
BASE COUNT          706 a         1253 c         1234 g          644 t
ORIGIN      
        1 accgcagcgc gcgcgactcg atcttccgcg ttcgttgcgg ctggtcggtt gcgggctgaa
       61 cgtcgttcaa gatgctccac aagtttccca ggccggtctt cctcaagcct gcgagatcgc
      121 ccgaatcggc acccaggcac gggacgggcc aaggttccta gactgtcccc atgcccacca
      181 tcgtccacga gtttgcctgg ccggatcgac tcgtggtcgg gaccgtcgga cgaccgggtg
      241 accgcgagtt ctacctgcag gcccgcacca aagcccggat cgtgaccatc gcccttgaga
      301 agcagcagtc agcgcagctc gcagagaaga tcgacgaagt actcgacgag ctgaggtcca
      361 ccgagggcaa ccccttccgg gttccggact cgatgcccgt cgaactcgtt gacaacgacc
      421 cacttgacca gccgatagac acccagttcc gcaccggcgc catgcgcctc ggctgggatc
      481 cgatgaccgc gcagatcgtc atcgaggcct acgccatgac cgacgctgac tacgacgagg
      541 acggcccaca gcccatcaaa atcgagccac cgagtcactg ctggtgcaca tgccggtagg
      601 catggcccgt gcattcgcaa tacgcaccct cgaggtggtc ggcgccggac gtcccagctg
      661 ctcactgtgc ggccagccca tgaacccgga gggacacctc tgcccgcaac ccggcgcact
      721 ctgacgaccg accccgactc tcagcacgcc gccggcgggt ctgacgccat gggaggccaa
      781 cacccggccc caagcgccag cttctgccgc aaccggcttc cggtgcgagc cacctccaac
      841 ctggctcctc caccgctggg atactgcaag ttgtgaggct gttcgacaag attcggatga
      901 ctgtcgatgt gctgaagtcg gtgcgtcgtc ccaccgtccc tctcgcggcg gcgcgggcca
      961 ggtacaaccc catggttcac acgcttgccg acgcattgac cgcggagttc ggcgtgacct
     1021 ggacggtggc gttcgaggcg gagttggttc cgttcggtcg gcgtggggac tacacaacgc
     1081 agaggctgat ctcaacagac cggttggacg cgttcccgga gtggtcagca cgctacgccg
     1141 acgtcgtgca gtcggtcgcc caggtcacgg gcttcggaac gcagggttac gacccgaagg
     1201 agtcgcaaca acgctccgag ccggccccat ctgagcggca gacctatgtg ctcgacgacg
     1261 acgtgggggt cgtcctcgag ccgatcgaca tacccgattc aggcccgtcg agaatcaccc
     1321 gggactcctt ggggggcatc atcgttctgg agatcgactc tgacggaacc ctcatcttca
     1381 ttgccgcagc agtccccgcc gacaatcacg aaacgcactg atctgcccgg gtagcgacgt
     1441 ttgcgagccc gactacccat gggaccggat cggtcgatta ggttgaacgc gtgaccgctg
     1501 agcagcctaa acctcgggtt ctcatcatcg ggctggatcc gcatcgcgtg ccaggcccct
     1561 gggaccctga gccggtggcc gaggccatca gagacggctt caccgagttc gccaagcacg
     1621 gcgtcgaagc acaggcctgc ctgttcggcc tcgacggcag cgacaatcca gaagacgtca
     1681 tagggacagt tctgcagtca caaccatggg aatgcgtggt cattggcggg ggtgtgcgca
     1741 cagacgacaa cctgctggac ctcttcgagc gaatcatcaa cctcgtgcgt cggctggccc
     1801 cgggcgctgc catcgcgttc aacgccagcc ccacagagac ctacgcagcc gcctcccgct
     1861 ggatcgacgt gggcgatgat cgctgaggcc gcggaggccc tcgggtttcg gtgactgcgc
     1921 agggccgccc gcgttgccaa ggggcctcag agttgcaggt gcagccgcag cgcgtcgtgg
     1981 agttgcttga tcaggtgggc gggaaggcgt ccgatcaccg tctgtcacga caggccggcc
     2041 agccggtaca gcaccagact cccggccacg gcaacgttga ggctggcgcc gacgccgcac
     2101 atgggaatct cgacgcactc gtcggccagg tcgaccatgt cgtccggaac cccgtggtgt
     2161 tcgtggccca acaacacgat cgtgggcagc cgcgcaggct ggaggcgagg cagaggcacg
     2221 gacccctcgg cgagttcgac ggccaccacc cgccacccct gttcctgcat cgatctgacc
     2281 cacggctcct tggccgtcac acgatgcagg cagggtctgc gatgcccgag cgtgttgccc
     2341 ttgctcaagg cctcgcgata gtgcggagtg tcaggcacgg ccaggcaggc tccgaccgca
     2401 tcgcaggtgc gttgcagcgt gcccaggttg gctccgtagg caacccacag gggcgccacg
     2461 atgaggtgcc cccagcaacc atgctgagta cggcggcgcc cacgacggag gtcggcgggc
     2521 gacgtgacgc ggcggctcat cgagccagaa gggatccgat cgggcggaaa acgatgctgt
     2581 gcatgtgtgg acgccattca gcggcggcgc cggtccatcg acggaggaag tgttgagtct
     2641 atccgaccca gtccttgccc gccgagtcgt atgagttcgc tcgatgctcc gcacctgatc
     2701 gggtcgaatg gggacctgtt tacgcgcaac cggcccaaaa cagagaggtc cagccgaacc
     2761 acgttcgacc agacctcaca gccttgcagc cccgacggga ttcgaacccg cgctaccgcc
     2821 ttgagaggga gcgcacaggc cagtcggcgc agttgggaag ccctgttcag gcttgtcagg
     2881 acacatttca gacgctggtg ccgttgggaa gccatggcgc cgcaagaagc tgatgcggga
     2941 tatctgcggg atgggcacag catccttagg gtcaggcgat gcccgatctc gcacctcccc
     3001 agacaccgaa actcctcggg gtcgggcgat gcgtccagcc gaaggtagtg tcgtgcgccg
     3061 aagccgccca ggacatgtgc gaccgacgga tccagcgaga cctccacaag cactgcggcg
     3121 gggctgacgc ccctcagtgc tcggggtcgc cggacacttc acaacggacc cagactgccc
     3181 acgagcacca gaaggcgcgg ctcgactcgc tgttcgccaa ggaccggcac gtcggcgtcg
     3241 aggtcacctg gccgctgcca agccaagatc atctaaccgc gaacccttgg cagagcgtgg
     3301 ccggtctccc gccgccattc ttgaagattg agcccgctgt ccagtgactc attacgagtg
     3361 tgttcagagg cactgatggg caatggatgc tagaagcggg cagcgtttga ggaggagcac
     3421 ctgccctagt cggggtgaca ttgcggctcg catggccgtc gacgcgccgc accatcaatc
     3481 ggcgcccaac ggagccaact gtgttatgga tgtgttatgc agccggcggc atccagagtt
     3541 ggtttgcgac cggtaaaggc ttgcctcgtc gcggcgcgac ccgcaatctc agactgggcc
     3601 caggacaccg ggagcaccag caccaaggag cccgaacgat tagtggcgtc cggactccgt
     3661 cgccggaaga tgtcaacctt gttgggtcag ttgatgcttg ggtttgttga gtttgggtcg
     3721 gctggtggct tgttgatcca ggcggcggcg ggtagggcgt gcgggacggg gtggtggcgg
     3781 actggtctga ttggctctca acggacagct gattttgatg gttcacgctg cttctga
//
LOCUS       DAOSUG010000292         3814 bp    DNA     linear   ENV 10-JUN-2024
DEFINITION  MAG TPA_asm: Micropruina sp. isolate AMR_MDS_2195
            NODE_9927_length_3814_cov_3.668263, whole genome shotgun sequence.
ACCESSION   DAOSUG010000292 DAOSUG010000000
VERSION     DAOSUG010000292.1
DBLINK      BioProject: PRJNA925477
            BioSample: SAMN32864262
            Sequence Read Archive: SRR4236673
KEYWORDS    WGS; ENV; Metagenome Assembled Genome; MAG; Third Party Data; TPA;
            TPA:assembly.
SOURCE      Micropruina sp. (wastewater metagenome)
  ORGANISM  Micropruina sp.
            Bacteria; Bacillati; Actinomycetota; Actinomycetes;
            Propionibacteriales; Nocardioidaceae; Micropruina.
REFERENCE   1  (bases 1 to 3814)
  AUTHORS   Abdulkadir,N., Saraiva,J.P., Zhang,J., Stolte,S., Gillor,O.,
            Harms,H. and Rocha,U.
  TITLE     Genome-centric analyses of 165 metagenomes show that mobile genetic
            elements are crucial for the transmission of antimicrobial
            resistance genes to pathogens in activated sludge and wastewater
  JOURNAL   Microbiol Spectr 12 (3), e0291823 (2024)
   PUBMED   38289113
REFERENCE   2  (bases 1 to 3814)
  AUTHORS   Abdulkadir,N.
  TITLE     Direct Submission
  JOURNAL   Submitted (24-MAR-2023) Environmental Microbiology, Helmholtz
            Center for Environmental Research -UFZ, Permoserstr 15, Leipzig
            04318, Germany
COMMENT     The annotation was added by the NCBI Prokaryotic Genome Annotation
            Pipeline (PGAP). Information about PGAP can be found here:
            https://www.ncbi.nlm.nih.gov/genome/annotation_prok/
            
            ##Genome-Assembly-Data-START##
            Assembly Method        :: SPAdes v. 3.11.1
            Genome Representation  :: Full
            Expected Final Version :: Yes
            Genome Coverage        :: Not Applicable
            Sequencing Technology  :: Illumina MiSeq
            ##Genome-Assembly-Data-END##
            
            ##Genome-Annotation-Data-START##
            Annotation Provider               :: NCBI
            Annotation Date                   :: 05/04/2023 04:57:10
            Annotation Pipeline               :: NCBI Prokaryotic Genome
                                                 Annotation Pipeline (PGAP)
            Annotation Method                 :: Best-placed reference protein
                                                 set; GeneMarkS-2+
            Annotation Software revision      :: 6.5
            Features Annotated                :: Gene; CDS; rRNA; tRNA; ncRNA
            Genes (total)                     :: 2,776
            CDSs (total)                      :: 2,750
            Genes (coding)                    :: 2,714
            CDSs (with protein)               :: 2,714
            Genes (RNA)                       :: 26
            tRNAs                             :: 24
            ncRNAs                            :: 2
            Pseudo Genes (total)              :: 36
            CDSs (without protein)            :: 36
            Pseudo Genes (ambiguous residues) :: 0 of 36
            Pseudo Genes (frameshifted)       :: 2 of 36
            Pseudo Genes (incomplete)         :: 32 of 36
            Pseudo Genes (internal stop)      :: 2 of 36
            ##Genome-Annotation-Data-END##
FEATURES             Location/Qualifiers
     source          1..3814
                     /organism="Micropruina sp."
                     /mol_type="genomic DNA"
                     /submitter_seqid="NODE_9927_length_3814_cov_3.668263"
                     /isolate="AMR_MDS_2195"
                     /isolation_source="Activated sludge of municipal
                     wastewater treatment plants"
                     /db_xref="taxon:2737536"
                     /environmental_sample
                     /geo_loc_name="Canada"
                     /lat_lon="49.25 N 123.1 W"
                     /collection_date="2015-06-16"
                     /metagenome_source="wastewater metagenome"
                     /note="metagenomic"
     gene            complement(1..208)
                     /locus_tag="PLF56_09740"
                     /pseudo
     CDS             complement(1..208)
                     /locus_tag="PLF56_09740"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_016665868.1"
                     /note="incomplete; partial in the middle of a contig;
                     missing N-terminus and C-terminus; Derived by automated
                     computational analysis using gene prediction method:
                     Protein Homology."
                     /pseudo
                     /codon_start=1
                     /transl_table=11
                     /product="serine/arginine repetitive matrix protein 2"
     gene            complement(253..2703)
                     /locus_tag="PLF56_09745"
     CDS             complement(253..2703)
                     /locus_tag="PLF56_09745"
                     /inference="COORDINATES: protein motif:HMM:NF012798.2"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_function: GO:0003677 - DNA binding [Evidence IEA];
                     GO_process: GO:0006310 - DNA recombination [Evidence IEA];
                     GO_process: GO:0015074 - DNA integration [Evidence IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="site-specific integrase"
                     /protein_id="HOQ53883.1"
                     /translation="MADHVDAVAFLPRGARPSKKLVGAHDFITPSLPTPAIARPTVWE
                     ASQQSVEEVLAVVEALEAGEAAHPKELARRLRSVEVLLGWLQRFGGESWQQRWLASGV
                     EVAGKAWPDLLAQSLTSELNLATLRAHATGGAGRLILADVIRPGYQWLYQASSWNLYA
                     RYEQVRDPEGFARMTARCVADPRIVSTDQRAALIQLCRILMHNGGLLADIGLADCVEA
                     YRAQTGYSARLHSHWYKLLCEEGILPVGAPPTIHAASRRGQLSIEEMVDGYDVACRPV
                     RNLFVDYLHQRSPGLDYGTIRQLASKLVLLFWRDLELHHPGIDSLHLSDDMARGWKQR
                     LQHVRYGNHRLGEERQDRYTILMSVRAFYSDLSHWALEEPGRWAAWAAPSPVDSRDVA
                     GMTKMTKHRQSRMHQRIRELAPLLPQLVDAAHTRRRQAAALLTAATSTPPGDTFTADS
                     LTLRRTALATDPTTGGTGRPGVVYATAADGSGPRRNLTLEADQAFWAWACVEVFRHTG
                     VRIEEMLEITHRSFVSYTLPTTGEVIPMLQITPSKTDKERLLVISPELAAAFAEIIHH
                     VRGSSPHLPLVSRYDGSERVHSPALPFLFQRRWGVRDIALNHGYVKGLLGGLVAAAGI
                     TNPDGSPAQFTPHDFRRIFATEAVSQGLPVHIAAKILGHDSLTTTQTYVAVYDTDVID
                     HHRAFIARRRATRPAQEYREPTDSEWDEFLGHWVARKLELGTCGRAYGTGCQHEHACI
                     RCPMLRPDPAQQPRLEAVIASLTERIAEATEHGWLGDVTGLTTSLDAANRKLVQMRRT
                     ATNLGLPARLGSSSQGAR"
     gene            complement(2696..>3814)
                     /locus_tag="PLF56_09750"
     CDS             complement(2696..>3814)
                     /locus_tag="PLF56_09750"
                     /inference="COORDINATES: protein motif:HMM:NF012798.2"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_function: GO:0003677 - DNA binding [Evidence IEA];
                     GO_process: GO:0006310 - DNA recombination [Evidence IEA];
                     GO_process: GO:0015074 - DNA integration [Evidence IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="tyrosine-type recombinase/integrase"
                     /protein_id="HOQ53884.1"
                     /translation="VVGAVATTDEVPGTALLDASGRPVVEVAEFFRGMLASGASESSL
                     RSYGLALLRWWRFLAAVEVPWDRAGRVEAQDFVLWMRMVGPAGRPGGYAPATINHGLA
                     VVKAFYADRLAAGRGPVVNPVPEAAHRDGRRVQAHHNPMQPFAPGPRAPLRQKVPERV
                     PRSIPDGLFDALFTAVRSDRDRALLAFWVSTGARAAELLGATLDRVDPGEQRIGVVRK
                     GTRRLQWLPASADAFVWWRLYEARVTRPAGERALWLTNRSPVRPLTYPAARRMLQRAN
                     AALGTVWTLHDLRHTAAQRMIDDPHLSLTDVQWVLGHAHLTTTQLYLRSRPDEVIAKV
                     LEHHRARAERPPAPRVPPGAPGYPADVLEALLGGGRRG"
BASE COUNT          590 a         1278 c         1359 g          587 t
ORIGIN      
        1 caccggtgtc ccggatgacc tgcaccgggt ccttgccgtc cacgttcgcc gcccacccgg
       61 cgacataggg gatcgtgtag tcgctggtgt ccatgccatg cgcagcggcg atcatcagcg
      121 cgacggactc ggcctcgacc tcaccgatcc cgcgatgctg gcgcgcttcc tgctggttcg
      181 gcccgtgcag acggacatgg cccagctctt ttccgcacaa ttttctggac gtgctggaca
      241 gggttgggtt catcatcttg ccccttgcga ggaggacccg aggcgggcgg gcaggccgag
      301 gttggtggca gtgcggcgca tctggacgag cttccggttg gcggcgtcaa ggctggtggt
      361 gagcccggtg acgtcgccga gccagccgtg ttcggtggcc tcagcgatac gttcggtgag
      421 gctggcgatg accgcttcga gccggggctg ttgcgcgggg tcgggtcgca gcatggggca
      481 gcggatgcag gcgtgttcgt gttggcagcc ggtgccgtag gcgcgtccgc aggtgccgag
      541 ttcgagcttg cgggcgaccc agtggccgag gaactcgtcc cattcgctgt cggtgggttc
      601 gcggtattcc tgggcgggcc gggtcgcgcg gcggcgggcg atgaacgcgc ggtggtggtc
      661 gatgacgtcg gtgtcgtaga cagcgacgta ggtctgagtg gtggtcagcg agtcatggcc
      721 cagaatcttg gcggcgatgt ggaccggcag gccctgggac acggcttcgg tggcgaagat
      781 gcggcggaag tcgtgcgggg tgaactgagc gggactgccg tcggggttgg tgataccggc
      841 tgcggcgacg agtccgccga ggagtccctt gacgtagccg tggttgaggg cgatgtcacg
      901 gactccccat cgtcgctgga acaagaacgg cagggcaggg ctgtggacac gttcggagcc
      961 gtcgtagcgg ctgaccagag gtaggtgggg gctgctgccg cggacgtggt ggatgatctc
     1021 ggcgaacgcc gcggcgagtt cggggctgat caccaggagt cgttccttgt cggtcttgga
     1081 cggcgtgatc tgcagcatcg ggatgacttc gccagtggtc ggtagcgtgt aggacacgaa
     1141 agatcggtgg gtgatctcga gcatttcctc gatccggacg ccggtgtggc ggaacacctc
     1201 gacgcaggcc cacgcccaga acgcttggtc ggcttccagg gtgaggttgc gccggggccc
     1261 gctgccatcg gcggcggtgg cgtagaccac gccgggtcgg ccggtgccgc ctgtggtggg
     1321 gtcggtagcc aaagcggtac ggcgcagggt gaggctgtcg gcggtgaagg tgtcaccggg
     1381 tggggtgctg gtcgcggcgg tgagcaacgc cgctgcctga cggcgacggg tgtgggcggc
     1441 gtcgaccagt tggggcagca ggggtgccag ttcgcggatc cgctggtgca tccgcgactg
     1501 gcggtgcttg gtcatcttgg tcatcccggc cacgtcgcgg gagtcgaccg gggacggcgc
     1561 cgcccacgca gcccaccgac ccggctcctc cagagcccag tggctgaggt cggagtagaa
     1621 ggcacggacg gacatcagga tggtgtaccg atcctggcgt tcctcgccga gcctgtggtt
     1681 gccgtagcgg acgtgctgga gtcgttgttt ccaacctcgg gccatgtcat cggacaggtg
     1741 gagggagtcg atgccggggt ggtggagttc caggtcccgc cagaacaaca gcaccagttt
     1801 cgaggcgagt tggcggatcg tgccgtagtc cagacccggg gatcgctggt gcaggtagtc
     1861 gacgaacagg ttccggaccg ggcggcacgc gacgtcatac ccgtcgacca tctcctcgat
     1921 gctgagctgc ccacgacggc tggccgcatg gatggtcggg ggcgcaccca cgggcaggat
     1981 gccctcctca cacaacagct tgtaccagtg ggaatggagt ctggcggagt agccggtttg
     2041 ggcacggtag gcctcaacgc agtcagccag cccaatgtcg gccagaaggc cgccgttgtg
     2101 catcaggatc cgacacaact ggatcaacgc ggcacgctgg tcggtgctga cgatccgcgg
     2161 gtcagccacg cagcgggcgg tcatcctggc gaacccctcg gggtcgcgga cctgctcgta
     2221 gcgggcgtac agattccacg acgacgcctg gtacagccac tggtagccgg gccggatcac
     2281 atcagccagg atcaaccgcc cagcaccgcc ggtcgcgtga gcgcgcaatg tcgctaggtt
     2341 cagctccgac gtgagtgatt gtgcgagcaa gtcgggccag gccttcccgg cgacctcaac
     2401 ccctgaggcc agccagcgtt gctgccacga ctcaccaccg aaccgctgca gccagcccaa
     2461 cagcacctcg acgctgcgaa gccgccgcgc caactccttg gggtgggctg cttcaccagc
     2521 ctctaacgcc tcgactaccg cgaggacctc ctcgacgctc tgctgggacg cctcccacac
     2581 cgtcggccgg gcgatcgcgg gggtcggcaa cgacggggtg atgaagtcgt gggcgccgac
     2641 caacttcttc gacggcctcg cgccgcgggg caggaacgcg accgcgtcca catggtcagc
     2701 cacgacgccc accacccagc aacgcctcca gcacgtccgc cgggtaaccg ggggcgcccg
     2761 gcggtacccg cggcgctggt ggccgttccg cgcgggcgcg gtggtgctcg agcaccttcg
     2821 cgatcacctc atcgggccgg gaccgcagat acagctgggt cgtggtcagg tgggcgtggc
     2881 ccagcaccca ctggacgtcg gtcagtgaca ggtgcgggtc gtcgatcatc cgctgcgcgg
     2941 cggtgtgacg caggtcatgc aacgtccaca cggtgcccaa cgcggcgttc gcgcgttgca
     3001 gcatccgccg ggccgccgga tacgtcaacg gacgcaccgg ggaccggttc gtcagccaca
     3061 acgcccgttc acccgccgga cgggtcaccc gggcctcgta cagccgccac cacacaaacg
     3121 cgtccgccga cgccggcagc cactgcagcc ggcgtgtccc cttccggacc accccgatcc
     3181 gctgctcacc cggatcgacc cggtccaagg tggcgcccag cagctccgcc gcccgggcgc
     3241 cggtcgacac ccagaacgcc aacaaggccc ggtcccgatc cgaacgcacc gccgtgaaca
     3301 aggcgtcgaa caggccatcg gggatgctcc gcggcacccg ctcgggcacc ttctgacgca
     3361 acggcgcccg tgggcccggc gcgaacggct gcatcgggtt gtgatgcgcc tgcacccgac
     3421 gaccgtcgcg atgcgcagcc tccgggaccg ggttcaccac agggccccgg ccggcggcca
     3481 gccggtcggc gtagaacgct ttcaccaccg ccagcccgtg gttgatcgtc gccggcgcgt
     3541 acccacccgg ccgtcccgcc gggccgacca tccgcatcca caacacgaag tcctgcgcct
     3601 cgacccgacc ggcccggtcc cacgggacct cgaccgcagc caggaaccgc caccagcgca
     3661 gcaacgccaa cccgtacgag cgcagcgagc tttcactcgc cccggaggcc agcatgccgc
     3721 ggaagaactc ggcgacctcc accaccggcc ggccggacgc gtccagcagg gccgtgcccg
     3781 gcacctcatc ggtcgtcgcc accgccccga cgac
//
LOCUS       DAOSUG010000293         3800 bp    DNA     linear   ENV 10-JUN-2024
DEFINITION  MAG TPA_asm: Micropruina sp. isolate AMR_MDS_2195
            NODE_9970_length_3800_cov_3.845394, whole genome shotgun sequence.
ACCESSION   DAOSUG010000293 DAOSUG010000000
VERSION     DAOSUG010000293.1
DBLINK      BioProject: PRJNA925477
            BioSample: SAMN32864262
            Sequence Read Archive: SRR4236673
KEYWORDS    WGS; ENV; Metagenome Assembled Genome; MAG; Third Party Data; TPA;
            TPA:assembly.
SOURCE      Micropruina sp. (wastewater metagenome)
  ORGANISM  Micropruina sp.
            Bacteria; Bacillati; Actinomycetota; Actinomycetes;
            Propionibacteriales; Nocardioidaceae; Micropruina.
REFERENCE   1  (bases 1 to 3800)
  AUTHORS   Abdulkadir,N., Saraiva,J.P., Zhang,J., Stolte,S., Gillor,O.,
            Harms,H. and Rocha,U.
  TITLE     Genome-centric analyses of 165 metagenomes show that mobile genetic
            elements are crucial for the transmission of antimicrobial
            resistance genes to pathogens in activated sludge and wastewater
  JOURNAL   Microbiol Spectr 12 (3), e0291823 (2024)
   PUBMED   38289113
REFERENCE   2  (bases 1 to 3800)
  AUTHORS   Abdulkadir,N.
  TITLE     Direct Submission
  JOURNAL   Submitted (24-MAR-2023) Environmental Microbiology, Helmholtz
            Center for Environmental Research -UFZ, Permoserstr 15, Leipzig
            04318, Germany
COMMENT     The annotation was added by the NCBI Prokaryotic Genome Annotation
            Pipeline (PGAP). Information about PGAP can be found here:
            https://www.ncbi.nlm.nih.gov/genome/annotation_prok/
            
            ##Genome-Assembly-Data-START##
            Assembly Method        :: SPAdes v. 3.11.1
            Genome Representation  :: Full
            Expected Final Version :: Yes
            Genome Coverage        :: Not Applicable
            Sequencing Technology  :: Illumina MiSeq
            ##Genome-Assembly-Data-END##
            
            ##Genome-Annotation-Data-START##
            Annotation Provider               :: NCBI
            Annotation Date                   :: 05/04/2023 04:57:10
            Annotation Pipeline               :: NCBI Prokaryotic Genome
                                                 Annotation Pipeline (PGAP)
            Annotation Method                 :: Best-placed reference protein
                                                 set; GeneMarkS-2+
            Annotation Software revision      :: 6.5
            Features Annotated                :: Gene; CDS; rRNA; tRNA; ncRNA
            Genes (total)                     :: 2,776
            CDSs (total)                      :: 2,750
            Genes (coding)                    :: 2,714
            CDSs (with protein)               :: 2,714
            Genes (RNA)                       :: 26
            tRNAs                             :: 24
            ncRNAs                            :: 2
            Pseudo Genes (total)              :: 36
            CDSs (without protein)            :: 36
            Pseudo Genes (ambiguous residues) :: 0 of 36
            Pseudo Genes (frameshifted)       :: 2 of 36
            Pseudo Genes (incomplete)         :: 32 of 36
            Pseudo Genes (internal stop)      :: 2 of 36
            ##Genome-Annotation-Data-END##
FEATURES             Location/Qualifiers
     source          1..3800
                     /organism="Micropruina sp."
                     /mol_type="genomic DNA"
                     /submitter_seqid="NODE_9970_length_3800_cov_3.845394"
                     /isolate="AMR_MDS_2195"
                     /isolation_source="Activated sludge of municipal
                     wastewater treatment plants"
                     /db_xref="taxon:2737536"
                     /environmental_sample
                     /geo_loc_name="Canada"
                     /lat_lon="49.25 N 123.1 W"
                     /collection_date="2015-06-16"
                     /metagenome_source="wastewater metagenome"
                     /note="metagenomic"
     gene            <1..1252
                     /locus_tag="PLF56_09755"
     CDS             <1..1252
                     /locus_tag="PLF56_09755"
                     /inference="COORDINATES: protein motif:HMM:NF014803.2"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=2
                     /transl_table=11
                     /product="Y4yA family PLP-dependent enzyme"
                     /protein_id="HOQ53885.1"
                     /translation="PRPLGRNADELVAAGRSAGVPIGLYFARKANKALSLVDEVVRRG
                     HGVDVASEPELRQVLQRGVAGDRIILTAAIKPERLLRLAVRAGVTISLDSPAEASVVA
                     GLAASIGMSARVAPRLAPEPELGLPPTRFGARAVHWRQLPPAGSSLEVRGVHFHLHGY
                     AAADRARMLAAALDLIKAVRERGHRPDFVDIGGGVPMSYLDDAAQWDAWWRAYRRSLA
                     AGDETLTWKNRELRSAYPFHQAPVRGEWLRDLLAAPLRTGSVADALRSAGLRLHLEPG
                     RALLDGCGLTLARVAFVKQRSDGVDLVGLEMNRTQCRSTSDDFLVDPILAGAASSGPP
                     LDAFLVGAYCIEDELILLRRLHFPQGVMPGDVIGLVNTGGYLMHILESASHQIPLARV
                     VVRTPDGFEPDPIDAWAEPGPGRG"
     gene            1412..3592
                     /gene="glgX"
                     /locus_tag="PLF56_09760"
     CDS             1412..3592
                     /gene="glgX"
                     /locus_tag="PLF56_09760"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_017933056.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_function: GO:0004133 - glycogen debranching enzyme
                     activity [Evidence IEA];
                     GO_process: GO:0005980 - glycogen catabolic process
                     [Evidence IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="glycogen debranching protein GlgX"
                     /protein_id="HOQ53886.1"
                     /translation="MHVWPGSPYPLGATFDGTGTNFALYSNVAERVELCLFDADRAET
                     RVELTEVDAKVWHCYLPQVEAGQRYGYRVYGPYDPARGLRCNPAKLLLDPYAKATEGT
                     IDWGQSLFSYTFGDEHSVNDDDSAPHMMLGVVTNPFFDWTGDRTLNVPYNESLIYEAH
                     VKGLTQLHPDVPEAMRGTYAGLIHPAVIAHFKRLGVTAVELMPVHQFVHDSVLVERGL
                     RNYWGYNTIGFFAPNAEYASSREAGTQVQEFKAMVRGLHAAGIEVILDVVYNHTAEGN
                     HLGPTLSMKGIDNPSYYRLVDETPHYYMDYTGTGNSLNVGNPHSLQLIMDSLRYWVTE
                     MHVDGFRFDLASALAREFYDVNRLSAFFELVQQDPVVSQVKLIAEPWDVGPGGYQVGN
                     FPPQWTEWNGKFRDAVRDFWLGQPNLGEFAARCAGSADLYEQSGRRPFASINFVTAHD
                     GFTLSDLVSYNEKHNEPNGENNNDGESHNRSWNHGVEGPTKDPAVLSARARSQRNLLA
                     TLLLSQGVPMLAHGDEAGRSQQGLNNGYAQDSPLTWFDWSAIDEPLLEFTATLAKLRA
                     DHPTFRRKRFFTGHRTDDGLDDIVWLHVSGRPMTDADWSSGAALGMYLNGSAIAGVNA
                     IGQPITDDDFLLYSNGGSEPVDAVLPGPEFGAAWDVVIDTAADDRLREALQSGSTVRI
                     EARSTLVLTEHVEAPTRPNLSAAASVAAITGQTADEPAPGTSPG"
     gene            3633..>3800
                     /locus_tag="PLF56_09765"
     CDS             3633..>3800
                     /locus_tag="PLF56_09765"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_008361628.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_function: GO:0016491 - oxidoreductase activity
                     [Evidence IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="FAD-dependent oxidoreductase"
                     /protein_id="HOQ53887.1"
                     /translation="MSNIASRCDAVVIGSGPNGLVAANALAQAGWHVVVIEARDRVGG
                     RTWTNEIDGVRL"
BASE COUNT          591 a         1306 c         1316 g          587 t
ORIGIN      
        1 tccccggccg ctgggtcgca acgccgacga actggtcgcg gccggacgta gcgccggggt
       61 gccgatcggg ctgtacttcg cgcgcaaggc caacaaggcg ctgagcctgg tcgacgaggt
      121 ggtgcggcgg gggcacgggg tggacgtggc cagcgagccc gaactgcggc aggttctgca
      181 gcgtggcgtc gccggggacc gcatcatcct gaccgctgcc atcaaacccg agcggctgtt
      241 gcggctggcc gtccgggccg gggtgacgat ttcgctggat tcaccggcgg aggcgtccgt
      301 ggttgccggg ctggccgcgt cgatcggcat gagcgcccgg gtcgctccgc ggctggcgcc
      361 cgagcccgaa ctgggactgc cgccgacccg gttcggtgct cgtgccgtgc actggaggca
      421 gctgccgccg gccggatcga gcctggaggt gaggggtgtg cactttcacc tgcacggcta
      481 cgccgccgcc gaccgtgccc gcatgctggc tgccgcgctc gacctgatca aggcggtgcg
      541 cgagcggggg caccgtccgg acttcgtcga catcggcggc ggggtgccga tgagctatct
      601 ggacgacgcc gcccaatggg acgcctggtg gcgcgcatac cgtcgcagcc tggccgccgg
      661 cgacgagacg ctcacctgga agaaccgcga actacgctcg gcatacccgt tccaccaggc
      721 gccggtgcgc ggtgagtggc tgcgcgacct gctggctgcg ccgttgcgga cgggcagcgt
      781 cgcggacgcg ctgcggtccg ccggcctgcg actgcacctg gagcccggac gggccctgct
      841 cgacggctgc ggcctgaccc tggcccgggt ggcgttcgtc aagcaacgca gcgacggggt
      901 ggacctggtc gggctcgaga tgaaccgcac ccagtgccgc tccacctctg acgatttcct
      961 ggtcgatccg attctggccg gcgccgcgtc gtccgggccg ccgctggacg cgttcctggt
     1021 cggcgcctac tgcatcgagg acgagctgat cctgctgcgc cgcctgcact ttccgcaggg
     1081 agtcatgccc ggtgacgtga tcgggctggt caacaccggc ggctacctga tgcacattct
     1141 ggagagcgcc tcgcaccaga ttccgctggc ccgggtggtc gtgcgcacgc cggacgggtt
     1201 cgagccggac ccgatcgatg cctgggctga gcccggaccc ggacggggct gagtcggcgg
     1261 gtcacgagcg gggcgagagc aggtgtccgg tgacccgtga cccgtggccc gtggaccgcc
     1321 tcccggggcc tgcagcgcac gatcaggact gtcaggtacg gctggtaggt tgctcgcgtt
     1381 cgtccgatca ccgttgaccc cgaggagatt cgtgcacgtc tggcccggtt cgccctatcc
     1441 gctcggtgcc accttcgacg gcaccggtac caacttcgcc ctgtacagca acgtggccga
     1501 gcgggtcgag ttgtgcctgt tcgacgccga ccgggccgaa acccgggtcg agctcaccga
     1561 ggtcgacgcc aaggtctggc actgctacct gccgcaggtc gaggcggggc agcgctacgg
     1621 ctaccgggtg tacgggccct acgacccggc ccgcgggttg cgctgcaacc ctgccaagct
     1681 gcttctcgac ccgtacgcca aggccaccga gggcaccatc gactggggcc agtcgttgtt
     1741 cagttacacc ttcggcgacg agcacagcgt caacgacgac gactcggcgc cgcacatgat
     1801 gctcggcgtg gtgaccaacc cgttcttcga ctggaccggc gatcgcaccc tcaacgtgcc
     1861 ctacaacgag agcctgatct acgaggccca cgtgaagggc ctgacgcaac tacaccccga
     1921 cgtgcccgag gcgatgcgcg gcacctacgc gggcctgatc catccggcgg tgatcgccca
     1981 cttcaagcgg ctgggggtca ccgccgtcga gttgatgccg gtgcaccagt tcgtacacga
     2041 ctcggtgctg gtcgaacgcg gcctgcgcaa ctactggggc tacaacacga tcggcttctt
     2101 cgccccgaac gccgagtacg cctcctcgcg cgaggcaggc acccaggtgc aggagttcaa
     2161 ggcgatggtg cgcgggctgc atgcggccgg catcgaggtg attctcgacg tggtctacaa
     2221 ccacaccgcc gagggcaacc acctggggcc gacgctcagc atgaagggca tcgacaaccc
     2281 gtcctattac cggctggtcg acgagacgcc gcattactac atggactaca ccggcaccgg
     2341 caactcgctc aacgtcggca acccccactc gctgcagctg atcatggatt cgttgcgcta
     2401 ctgggtgacc gagatgcacg tggacgggtt ccgcttcgac ctggcctcgg cgctggcgcg
     2461 cgagttctac gacgtcaacc ggctgtcggc cttcttcgag ctcgtgcagc aggacccggt
     2521 ggtcagccag gtcaagctga tcgccgagcc gtgggacgtc gggcccggcg gctatcaggt
     2581 gggcaacttc cccccgcaat ggacggagtg gaacggcaag ttccgcgacg ccgtgcgcga
     2641 cttctggctg gggcagccca acctgggcga gttcgctgcg cgctgcgcgg gcagcgccga
     2701 cctgtacgag cagtccgggc gtcgtccgtt cgccagcatc aacttcgtca ccgcccacga
     2761 cgggttcacc ctcagcgacc tcgtgtcgta caacgagaag cacaatgagc ccaacggcga
     2821 gaacaacaac gacggcgaat cgcacaaccg ctcgtggaac cacggcgtcg aagggccgac
     2881 caaagacccg gccgtactgt cggcgcgcgc ccgctcgcag cgcaacctgc tggccaccct
     2941 gctgctcagc cagggcgtgc cgatgctcgc gcacggcgac gaggcgggcc gcagccagca
     3001 aggcctcaac aacgggtacg cgcaggattc gccgctcacc tggttcgact ggtcggccat
     3061 cgacgagccg ctgctcgagt tcaccgccac cctggcgaag ttgcgggccg atcatccgac
     3121 gttccggcgc aagcggttct tcaccggtca ccgcaccgac gacgggctgg acgacatcgt
     3181 ctggctgcac gtcagcggac gtccgatgac cgatgccgac tggtcgagcg gtgccgccct
     3241 cggcatgtac ctgaacggat cggccatcgc cggtgtgaac gccatcggcc aacccatcac
     3301 cgacgacgac tttctgctgt attccaacgg cggctccgag ccggtggacg cggtgctgcc
     3361 cggccccgag ttcggcgccg cgtgggacgt ggtgatcgac accgcggccg atgatcggct
     3421 tcgcgaagct ctgcagtcgg ggtcgacggt gcgcatcgag gcacgcagca cgctggtgct
     3481 gaccgagcat gtcgaggcgc cgacccgtcc gaacctgtcg gcggcggctt cggttgcggc
     3541 gatcacgggg cagaccgccg acgaaccggc ccccggcacc tcacccggct gagtgggtgt
     3601 gcaattgcgg cgaacagggt aggaaggcag gcatgagcaa catcgcgtcc cgctgtgatg
     3661 ccgtggtgat cggttcgggc ccgaacggat tggtggctgc gaacgcgttg gcgcaggccg
     3721 gttggcacgt ggtggtgatc gaggcccgcg accgcgtcgg cggccgcacg tggaccaacg
     3781 agatcgacgg cgtccgcctc
//
LOCUS       DAOSUG010000294         3788 bp    DNA     linear   ENV 10-JUN-2024
DEFINITION  MAG TPA_asm: Micropruina sp. isolate AMR_MDS_2195
            NODE_10011_length_3788_cov_5.405840, whole genome shotgun sequence.
ACCESSION   DAOSUG010000294 DAOSUG010000000
VERSION     DAOSUG010000294.1
DBLINK      BioProject: PRJNA925477
            BioSample: SAMN32864262
            Sequence Read Archive: SRR4236673
KEYWORDS    WGS; ENV; Metagenome Assembled Genome; MAG; Third Party Data; TPA;
            TPA:assembly.
SOURCE      Micropruina sp. (wastewater metagenome)
  ORGANISM  Micropruina sp.
            Bacteria; Bacillati; Actinomycetota; Actinomycetes;
            Propionibacteriales; Nocardioidaceae; Micropruina.
REFERENCE   1  (bases 1 to 3788)
  AUTHORS   Abdulkadir,N., Saraiva,J.P., Zhang,J., Stolte,S., Gillor,O.,
            Harms,H. and Rocha,U.
  TITLE     Genome-centric analyses of 165 metagenomes show that mobile genetic
            elements are crucial for the transmission of antimicrobial
            resistance genes to pathogens in activated sludge and wastewater
  JOURNAL   Microbiol Spectr 12 (3), e0291823 (2024)
   PUBMED   38289113
REFERENCE   2  (bases 1 to 3788)
  AUTHORS   Abdulkadir,N.
  TITLE     Direct Submission
  JOURNAL   Submitted (24-MAR-2023) Environmental Microbiology, Helmholtz
            Center for Environmental Research -UFZ, Permoserstr 15, Leipzig
            04318, Germany
COMMENT     The annotation was added by the NCBI Prokaryotic Genome Annotation
            Pipeline (PGAP). Information about PGAP can be found here:
            https://www.ncbi.nlm.nih.gov/genome/annotation_prok/
            
            ##Genome-Assembly-Data-START##
            Assembly Method        :: SPAdes v. 3.11.1
            Genome Representation  :: Full
            Expected Final Version :: Yes
            Genome Coverage        :: Not Applicable
            Sequencing Technology  :: Illumina MiSeq
            ##Genome-Assembly-Data-END##
            
            ##Genome-Annotation-Data-START##
            Annotation Provider               :: NCBI
            Annotation Date                   :: 05/04/2023 04:57:10
            Annotation Pipeline               :: NCBI Prokaryotic Genome
                                                 Annotation Pipeline (PGAP)
            Annotation Method                 :: Best-placed reference protein
                                                 set; GeneMarkS-2+
            Annotation Software revision      :: 6.5
            Features Annotated                :: Gene; CDS; rRNA; tRNA; ncRNA
            Genes (total)                     :: 2,776
            CDSs (total)                      :: 2,750
            Genes (coding)                    :: 2,714
            CDSs (with protein)               :: 2,714
            Genes (RNA)                       :: 26
            tRNAs                             :: 24
            ncRNAs                            :: 2
            Pseudo Genes (total)              :: 36
            CDSs (without protein)            :: 36
            Pseudo Genes (ambiguous residues) :: 0 of 36
            Pseudo Genes (frameshifted)       :: 2 of 36
            Pseudo Genes (incomplete)         :: 32 of 36
            Pseudo Genes (internal stop)      :: 2 of 36
            ##Genome-Annotation-Data-END##
FEATURES             Location/Qualifiers
     source          1..3788
                     /organism="Micropruina sp."
                     /mol_type="genomic DNA"
                     /submitter_seqid="NODE_10011_length_3788_cov_5.405840"
                     /isolate="AMR_MDS_2195"
                     /isolation_source="Activated sludge of municipal
                     wastewater treatment plants"
                     /db_xref="taxon:2737536"
                     /environmental_sample
                     /geo_loc_name="Canada"
                     /lat_lon="49.25 N 123.1 W"
                     /collection_date="2015-06-16"
                     /metagenome_source="wastewater metagenome"
                     /note="metagenomic"
     gene            complement(<1..212)
                     /locus_tag="PLF56_09770"
     CDS             complement(<1..212)
                     /locus_tag="PLF56_09770"
                     /inference="COORDINATES: ab initio
                     prediction:GeneMarkS-2+"
                     /note="Derived by automated computational analysis using
                     gene prediction method: GeneMarkS-2+."
                     /codon_start=1
                     /transl_table=11
                     /product="hypothetical protein"
                     /protein_id="HOQ53888.1"
                     /translation="MRVVIAGSGSPLGDATASALRASGHEAIEIGPAECDLRDFAAVQ
                     ALAEQLGAVDGVIHLVGGWRGGGGLAG"
     gene            complement(212..1588)
                     /locus_tag="PLF56_09775"
     CDS             complement(212..1588)
                     /locus_tag="PLF56_09775"
                     /inference="COORDINATES: protein motif:HMM:NF039440.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="DUF6421 family protein"
                     /protein_id="HOQ53889.1"
                     /translation="MTTVAEIEVSQLAQSPAWLRVKAAASALQPLQAANGSIENPETH
                     DRARQLVGDLVGGIAELAPQFPHDADYLDAVRHDFARWAEDGFGVPDFYDSLVLFQPQ
                     QHRVDGLTHLVVFPMYTQNGSRNRLVEAVLVQVIWPDFVAELEASDYSNKLFVPIRFL
                     DFTAGYDTNAAVLFPETVAMRQIPTFTWGAIFADREAARFRRVLRAAVDVTKLDLPAE
                     AAELLDDQQLAEETFVMWDLIHDRTHMRGDLPFDPFMIKQRMPFYLYSLEELRCDLTA
                     FRESVAIDRRIAALAEPTDDQLELARHARLVQYCVIFDRIFRFAITGTRVRNYDGLGG
                     QLLFAWLHQHHVLHWTDTQLAFEWSQVPDCVNELGAAIEHLYWRSIDRPKVAHWLAAY
                     ELVSGTVEPHPASVWKTGDLPLNGLPRELTDLIMDDEFPLSMFYEAFEKKMRDVIAST
                     SGITGTSV"
     gene            1882..2415
                     /locus_tag="PLF56_09780"
     CDS             1882..2415
                     /locus_tag="PLF56_09780"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_013862090.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="peptidase"
                     /protein_id="HOQ53890.1"
                     /translation="MQSEDDRSVEVSPFQAQGVLSGFLVSGRWPANTVEWARFLLLAV
                     RVASVPGMLPTSTVFRVREERPESPHPGAVGLVLAEGPVVGQNAVLPGQFAAPPPPGL
                     IVLHPPSEIRPSLPEQDEVAAGCVLLPGIPHLGLEHRAAWVEADRRGNIARMVSRAGI
                     DPNEDADTAVLAMFLAA"
     gene            complement(2496..3479)
                     /locus_tag="PLF56_09785"
     CDS             complement(2496..3479)
                     /locus_tag="PLF56_09785"
                     /EC_number="5.1.3.-"
                     /inference="COORDINATES: protein motif:HMM:NF013431.2"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_function: GO:0016857 - racemase and epimerase activity,
                     acting on carbohydrates and derivatives [Evidence IEA];
                     GO_function: GO:0030246 - carbohydrate binding [Evidence
                     IEA];
                     GO_process: GO:0005975 - carbohydrate metabolic process
                     [Evidence IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="aldose epimerase family protein"
                     /protein_id="HOQ53891.1"
                     /translation="MTEHPSTHPFGSIDGRAVERVRLQSGGLTVDLITLGAAVHRVIV
                     NGRDVVLGHPDADGYADSQFCLGFTIGRFANRIGAGRFTLDGAEHQLATNEGDNTLHG
                     GPDGFHHRLWTIDDLTADSVRFGLVSPAGDQGFPGELTASVRYTIAPDELRQDYLATT
                     TAPTPVNFTNHSYFNLAGEASGTTDRQTVWVPARWVVEVDDELIPTGRLVPAGTHDLR
                     TPRAIAEAAPLDTCFVVGGEPGTLRAHATMASDDLALDVFSDQPGIQLYTADAMDGVP
                     GLNGPYGARAGLALETQNLPDAPNHPDFPNSILRPGDEFRSATIWRFRTLP"
     gene            complement(3487..>3788)
                     /locus_tag="PLF56_09790"
     CDS             complement(3487..>3788)
                     /locus_tag="PLF56_09790"
                     /inference="COORDINATES: ab initio
                     prediction:GeneMarkS-2+"
                     /note="Derived by automated computational analysis using
                     gene prediction method: GeneMarkS-2+."
                     /codon_start=3
                     /transl_table=11
                     /product="hypothetical protein"
                     /protein_id="HOQ53892.1"
                     /translation="WDELFFICTVFALYRRHFRLWQANLLQAMVFVSFLWELGYQSWG
                     PLLTIPFALVQGAIFQLTRNLAYVITVHLLFDVVVFLVLIHGHNPTWPHIFLTVP"
BASE COUNT          614 a         1213 c         1371 g          590 t
ORIGIN      
        1 ccggccagcc cgccgccccc gcgccatccg ccgaccaggt ggatcacccc gtccacggcg
       61 ccgagctgtt cggccagggc ctggacggcc gcgaagtccc gcaggtcgca ctctgccggc
      121 ccgatctcga tcgcctcgtg cccggacgcg cgcagtgcgg acgccgtcgc gtcgcccagc
      181 gggctgccgg aacctgcgat gaccacccgc atcagacgga ggttccggtg atgcccgagg
      241 tggacgcgat cacatcgcgc atcttcttct cgaacgcctc gtagaacatg ctgagcggaa
      301 actcgtcgtc catgatcagg tccgtcagct cgcgggggag tccgttgagc ggcaggtcgc
      361 cggtcttcca caccgaggcg ggatgcggct cgacggtgcc ggagaccagc tcgtaggcgg
      421 ccagccagtg ggcgaccttg ggccggtcga tcgaacgcca gtacaggtgc tcgatggcgg
      481 cgccgagttc attgacgcag tcgggcacct ggctccactc gaaggcgagc tgggtgtcgg
      541 tccagtgcag cacgtgatgc tggtgcagcc aggcgaacag cagctggccg cccaggccgt
      601 cgtagttgcg tacccgggtg ccggtgatcg cgaaccggaa gatgcggtcg aagatcacgc
      661 agtactgcac caggcgggcg tgccgggcga gttcgagttg gtcgtcggtc ggctcggcga
      721 gcgcggcgat ccgtcgatcg atggcgacgg attcgcgaaa ggcggtcagg tcgcagcgca
      781 actcttcgag cgagtagagg tagaacggca tccgctgctt gatcatgaac gggtcgaacg
      841 gcaggtcgcc gcgcatgtgg gtgcggtcgt ggatcagatc ccacatgacg aacgtctctt
      901 cggccagttg ctggtcgtcg agcagttcgg cggcctcggc gggcaggtcg agcttggtga
      961 cgtccacggc ggcgcgcagc acccggcgga accgggccgc ctcgcggtcg gcgaagatcg
     1021 caccccaggt gaacgtgggg atctgccgca tggcgacggt ttcggggaag agcacggccg
     1081 cgttggtgtc gtagcccgcg gtgaagtcga ggaaccggat cggcacgaac agcttgttgc
     1141 tgtagtcgga cgcctcgagt tcggccacga agtcgggcca gatgacctgc acgagcacgg
     1201 cctcgaccag gcggttacgc gagccgttct gggtgtacat gggaaagacg accaggtggg
     1261 tcaggccgtc cacgcggtgc tgctgcggct ggaacagcac cagcgagtcg tagaagtcgg
     1321 gcacgccgaa gccgtcctcg gcccagcggg cgaagtcgtg ccgcacggcg tccaagtagt
     1381 cggcgtcgtg gggaaactgg ggtgccagtt cggcgatgcc gccgaccagg tcgccgacga
     1441 gctggcgggc gcggtcgtgg gtctcaggat tctcgatgga accgttggcc gcctgcaacg
     1501 gttgcagcgc cgaggcggcg gccttgactc ggagccacgc ggggctctgg gcgagctgcg
     1561 acacttcaat ctctgcgacg gtggtcatgg cagattctca catgatggac gccatttgcg
     1621 ccagcatcgc cgccacacct ggatatttgc ctgtgtaatc aacgcgaaat cgagctgaaa
     1681 caaacaattc ttttgtgagc gtggctctgg gcgggctgcc tcgaccggta tcaccagagt
     1741 atcggacggg ctcctgcacc cgtccgcaca ccggcgcgag agccgtcagg cgggcagctt
     1801 ggcactcggg gggtcttgtg tgccaacact gttgtgattc accatggcgc aactccgaca
     1861 cgcgagcgca ggaggctgat catgcagagc gaagacgatc gttcggtcga ggtgtcgccc
     1921 tttcaggccc agggcgtgct cagcggcttt ctggtgtcgg gccggtggcc ggccaacacc
     1981 gtggagtggg cgcgcttcct gctgctcgcc gtccgggtgg ccagcgtgcc gggcatgctg
     2041 cccaccagca cggtgttccg ggtgcgcgaa gagcgacccg agtcgcccca cccgggggcg
     2101 gtcggcctgg tgctcgccga ggggccggtg gtcggccaga acgcggtgct gcccggccag
     2161 ttcgccgccc cgccaccgcc ggggctgatc gtgctgcatc cgccgagcga gatccgtccc
     2221 tcactgcccg agcaggacga ggtcgcggcc ggctgtgtgc tgctgcccgg cattccgcac
     2281 ctgggcctgg agcaccgggc cgcctgggtc gaggcggacc gccggggcaa catcgcccgc
     2341 atggtcagca gggccggcat cgaccccaac gaggacgccg acaccgccgt gctggcgatg
     2401 ttcctggccg cctgaggggt tctcggggcc gccgcccggc gtgatcgagc ggctatcaca
     2461 cgtgcctgct cgacgaagat tcgaccgagg ccgcctcagg gcagggtgcg aaaccgccag
     2521 atggtggcgc ttcgaaactc gtccccgggc cgcaggatcg agttcggaaa gtcggggtgg
     2581 ttgggggcgt ccggcaggtt ctgggtctcg agcgccagcc cggcacgggc accgtacggg
     2641 ccgttcagcc cgggcacccc gtccatcgcg tcggcggtgt acagctggat tccgggctgg
     2701 tcgctgaaca cgtccagggc caggtcgtcg ctcgccatgg tggcgtgcgc gcgcagggtg
     2761 cccggctcgc caccgaccac gaaacaagtg tcgagcggtg ccgcctcggc gatcgcgcgg
     2821 ggcgtccgca ggtcgtgggt gcccgccggc accagccgtc cggtcgggat cagctcgtcg
     2881 tcgacctcga ccacccagcg ggcaggcacc cacaccgtct gccgatcggt ggtgccggag
     2941 gcctcaccgg ccaggttgaa gtagctgtgg ttggtgaagt tgaccggcgt gggtgccgta
     3001 gtggtggcga ggtagtcctg gcgcagttcg tccggggcga tcgtgtagcg cacggacgcg
     3061 gtgagttcgc ccggaaagcc ctgatcgccc gcggggctga ccaggccgaa gcgcaccgag
     3121 tcggcggtga ggtcgtcgat cgtccagagc cggtggtgaa agccgtcggg gccgccgtgc
     3181 agggtgttgt cgccctcgtt ggtcgccagc tggtgctcgg ccccgtccaa agtgaagcgg
     3241 ccggcgccga tgcggttggc gaagcgtccg atcgtgaaac cgaggcagaa ctgcgaatcg
     3301 gcgtagccgt ccgcgtccgg gtggcccagc acgacgtcgc ggccgttcac gatcacccgg
     3361 tgcacggcgg cgcccagcgt gatcaggtcg acggtcaggc cgcccgactg cagccggacg
     3421 cgctcgacgg cgcggccgtc gatgctgccg aacggatggg tggacgggtg ctcggtcatc
     3481 ggactcctac ggaacggtca agaaaatgtg cggccacgtc ggattgtgcc cgtgaatcag
     3541 caccagaaag accacaacgt cgaaaagtag gtgcacggtg atcacgtagg ccagattcct
     3601 ggtcagctga aagatcgcgc cctgcaccag cgcgaacgga atggtgagca gcggacccca
     3661 cgattgatag cccaactccc acagaaacga cacgaacacc atcgcctgca gcaggttggc
     3721 ctgccagagc cgaaagtgcc gccggtacag ggcgaagacg gtgcagatga agaacagctc
     3781 gtcccaga
//
LOCUS       DAOSUG010000295         3779 bp    DNA     linear   ENV 10-JUN-2024
DEFINITION  MAG TPA_asm: Micropruina sp. isolate AMR_MDS_2195
            NODE_10041_length_3779_cov_5.175886, whole genome shotgun sequence.
ACCESSION   DAOSUG010000295 DAOSUG010000000
VERSION     DAOSUG010000295.1
DBLINK      BioProject: PRJNA925477
            BioSample: SAMN32864262
            Sequence Read Archive: SRR4236673
KEYWORDS    WGS; ENV; Metagenome Assembled Genome; MAG; Third Party Data; TPA;
            TPA:assembly.
SOURCE      Micropruina sp. (wastewater metagenome)
  ORGANISM  Micropruina sp.
            Bacteria; Bacillati; Actinomycetota; Actinomycetes;
            Propionibacteriales; Nocardioidaceae; Micropruina.
REFERENCE   1  (bases 1 to 3779)
  AUTHORS   Abdulkadir,N., Saraiva,J.P., Zhang,J., Stolte,S., Gillor,O.,
            Harms,H. and Rocha,U.
  TITLE     Genome-centric analyses of 165 metagenomes show that mobile genetic
            elements are crucial for the transmission of antimicrobial
            resistance genes to pathogens in activated sludge and wastewater
  JOURNAL   Microbiol Spectr 12 (3), e0291823 (2024)
   PUBMED   38289113
REFERENCE   2  (bases 1 to 3779)
  AUTHORS   Abdulkadir,N.
  TITLE     Direct Submission
  JOURNAL   Submitted (24-MAR-2023) Environmental Microbiology, Helmholtz
            Center for Environmental Research -UFZ, Permoserstr 15, Leipzig
            04318, Germany
COMMENT     The annotation was added by the NCBI Prokaryotic Genome Annotation
            Pipeline (PGAP). Information about PGAP can be found here:
            https://www.ncbi.nlm.nih.gov/genome/annotation_prok/
            
            ##Genome-Assembly-Data-START##
            Assembly Method        :: SPAdes v. 3.11.1
            Genome Representation  :: Full
            Expected Final Version :: Yes
            Genome Coverage        :: Not Applicable
            Sequencing Technology  :: Illumina MiSeq
            ##Genome-Assembly-Data-END##
            
            ##Genome-Annotation-Data-START##
            Annotation Provider               :: NCBI
            Annotation Date                   :: 05/04/2023 04:57:10
            Annotation Pipeline               :: NCBI Prokaryotic Genome
                                                 Annotation Pipeline (PGAP)
            Annotation Method                 :: Best-placed reference protein
                                                 set; GeneMarkS-2+
            Annotation Software revision      :: 6.5
            Features Annotated                :: Gene; CDS; rRNA; tRNA; ncRNA
            Genes (total)                     :: 2,776
            CDSs (total)                      :: 2,750
            Genes (coding)                    :: 2,714
            CDSs (with protein)               :: 2,714
            Genes (RNA)                       :: 26
            tRNAs                             :: 24
            ncRNAs                            :: 2
            Pseudo Genes (total)              :: 36
            CDSs (without protein)            :: 36
            Pseudo Genes (ambiguous residues) :: 0 of 36
            Pseudo Genes (frameshifted)       :: 2 of 36
            Pseudo Genes (incomplete)         :: 32 of 36
            Pseudo Genes (internal stop)      :: 2 of 36
            ##Genome-Annotation-Data-END##
FEATURES             Location/Qualifiers
     source          1..3779
                     /organism="Micropruina sp."
                     /mol_type="genomic DNA"
                     /submitter_seqid="NODE_10041_length_3779_cov_5.175886"
                     /isolate="AMR_MDS_2195"
                     /isolation_source="Activated sludge of municipal
                     wastewater treatment plants"
                     /db_xref="taxon:2737536"
                     /environmental_sample
                     /geo_loc_name="Canada"
                     /lat_lon="49.25 N 123.1 W"
                     /collection_date="2015-06-16"
                     /metagenome_source="wastewater metagenome"
                     /note="metagenomic"
     gene            373..1014
                     /gene="pdxH"
                     /locus_tag="PLF56_09795"
     CDS             373..1014
                     /gene="pdxH"
                     /locus_tag="PLF56_09795"
                     /EC_number="1.4.3.5"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_016668102.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_function: GO:0004733 - pyridoxamine-phosphate oxidase
                     activity [Evidence IEA];
                     GO_function: GO:0010181 - FMN binding [Evidence IEA];
                     GO_process: GO:0008615 - pyridoxine biosynthetic process
                     [Evidence IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="pyridoxamine 5'-phosphate oxidase"
                     /protein_id="HOQ53893.1"
                     /translation="MTSQSILGLRKSYEQGELDEEAATNKPLEQFAAWFADARRIKGL
                     EPNAMTLATASADGRPSARVVLLKEFDASGLVWYTNYDSRKGAELDANPVASLLFFWA
                     PLERQVRVEGRVEKVTPEESDAYYAVRPLDSRIGAWASPQSQVIANRGVLVANAAKAA
                     AEHGLHPLRPDHWGGYRLVPEVWEFWQGRTSRLHDRLRYRLEGGDWVRERLAP"
     gene            1055..2398
                     /locus_tag="PLF56_09800"
     CDS             1055..2398
                     /locus_tag="PLF56_09800"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_020389825.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_function: GO:0008324 - monoatomic cation transmembrane
                     transporter activity [Evidence IEA];
                     GO_process: GO:0006812 - monoatomic cation transport
                     [Evidence IEA];
                     GO_process: GO:0055085 - transmembrane transport [Evidence
                     IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="potassium transporter TrkG"
                     /protein_id="HOQ53894.1"
                     /translation="MLGFARWLSHPGRIAPVAYLFGWVVGTLVLMSPAATESGESSGF
                     WVAAFTAMSALCITGLAVVDTPSHWSTFGELTIMTLIQVGGLGIMTLTSLIIFSVTRR
                     ASQAQLHIAQSETRARTRRGLRSIPARILMLSLAFELVFAVVLTLRFRSYLPDWGAAA
                     YHGMFHAVSAFNNAGFALYPDNLVRFNADPFVMVPICLAIVLGGLGFPVYLEVVERVR
                     GHLPPFWSVHLRLTLYGTVILLVLGFATFAAFEWTNPATLGGQGPMGKFLGALGGSVF
                     PRTAGFNSVDYALVTDETLAVDFGLMMIGGGSGGTAGGLKVTTVAVLLLAVITEVRGH
                     EKTVLGSRKISSAVSRQALAVVVLGTAAVFVGILAISAVSDAPLREDTFEAISAFGTV
                     GLSMNLTPTLPPAAWGVLMGLMYLGRVGPVSVAAALAMTARHRHYELPREDPLVG"
     gene            2391..3065
                     /locus_tag="PLF56_09805"
     CDS             2391..3065
                     /locus_tag="PLF56_09805"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_007078289.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_process: GO:0006813 - potassium ion transport [Evidence
                     IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="TrkA family potassium uptake protein"
                     /protein_id="HOQ53895.1"
                     /translation="MDKLFRPRPADGMVLVLGLGRFGIACARRLQELGMDVLGVDADL
                     DLVNRYADTLRHVVRMDSTDADALRQLGLENVGIAIVSMAHLESSIITLLTLKEEGVP
                     EVWAKASSRKHGELLKRIGADHIVYPERSAGERVAHTVAGHMIDYFEFEDGFAMARTL
                     APSFAWGRSLAESAIRTRHDVTVVGLKRPGEDFIYAVPETVVLEGDQLIVSGSKRDVE
                     RFARIS"
     gene            complement(3132..>3779)
                     /locus_tag="PLF56_09810"
     CDS             complement(3132..>3779)
                     /locus_tag="PLF56_09810"
                     /inference="COORDINATES: protein motif:HMM:NF033580.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_function: GO:0004803 - transposase activity [Evidence
                     IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="IS5 family transposase"
                     /protein_id="HOQ53896.1"
                     /translation="IGRSRGGLTTKNHLVSDGRGRVLAFILTPGQSADTSMLIQTMEQ
                     VRVPTSGRARTRPDRLLADKGYPSKANRTWLRRHGIATTIPERDDQIAHRRKKPGRPI
                     DFGEAQRERYKGRNVVERCFNKLKQWRGLAMRSDKYARNYHAGICLAATLDWLRTGFS
                     NTPYDRARNRGCRHPHAARILAQGWTRILWRCWHDHQPYNPDLHGRLNTLQQEAA"
BASE COUNT          534 a         1241 c         1300 g          704 t
ORIGIN      
        1 cggaaggtgt cgatctgcgt ttctccgctc acgcggtaga tgttcgtgaa gactggacgg
       61 cgttcagggc cctgtacgac ttggcctgca cctcggacga gcggctgacg gctctgcgtg
      121 aggcaggtta tggcttccag atcgtgacgt cgacggaaca cacccctgaa gaggtcgcgg
      181 agtacaggca atggttctcg gagatcgacc taggcgactc ggaggctcag gccgagtact
      241 tgtatcacac ggttctgctc gaagttattg acatagacgc tgttgaggtg actgaggcag
      301 atctggttga gttcaccgga gagttcccat ccatcaagaa ctggcgtcga gcaacacagt
      361 cgctgtggat ggttgacttc tcagagtatc ctcggactcc gcaagagcta cgagcagggc
      421 gaactggacg aggaagccgc caccaacaag ccgctggagc agttcgcggc ctggttcgcc
      481 gacgcacgcc gcatcaaggg gctcgagccg aacgcgatga ccctggccac ggcgtcggcc
      541 gacgggcggc cgtcggcccg ggtggtgctg ctgaaggagt tcgatgcgtc cgggctggtc
      601 tggtacacga actacgactc gcgcaagggc gccgaattgg acgccaaccc cgtcgcctcc
      661 ctgctgttct tctgggcgcc gctggagcgc caggtgcgcg tggaggggcg cgtggagaag
      721 gtcacgcccg aggagtcgga cgcctactac gcggtgcgcc cgctcgacag ccgcatcggc
      781 gcgtgggcct cgccccagtc gcaggtgatc gccaaccggg gcgtgctggt cgccaacgcg
      841 gccaaggctg cggcagagca cgggctgcac ccgctgcgtc cggaccactg gggtggctac
      901 cgcctcgtgc ccgaggtctg ggagttctgg cagggccgca cctcgcgcct gcacgaccgg
      961 ctgcgctacc gcctcgaggg cggcgactgg gtgcgggagc ggctggcgcc gtagcgccca
     1021 ccggacccac ggtgggggga gtagtgttct ccgcgtgctc ggctttgcgc ggtggctgtc
     1081 ccaccccgga cgcatcgccc cggtcgcgta cctgttcggg tgggtggtcg ggacgctcgt
     1141 gctgatgtct cccgccgcca ccgagtccgg cgagtccagc gggttctggg tggctgcgtt
     1201 cacggccatg tcggcgctgt gcatcaccgg tctggcggtg gtcgacaccc cgagccactg
     1261 gagcaccttc ggtgagctca ccatcatgac cctcatccag gtcggtgggc tggggatcat
     1321 gaccctgacc agcctgatca tcttctcggt cacccgacgg gcttcgcagg cccaactgca
     1381 catcgcccag tcggagacgc gggcgcgcac cagacggggg ctgcggtcga tcccagcccg
     1441 gatcctgatg ctgtcactgg cgttcgagct cgtgttcgcg gtcgtgctga cgctccggtt
     1501 ccggtcctac ctcccggact ggggagcggc ggcctaccac ggcatgttcc acgcggtgtc
     1561 tgcgttcaac aacgccggat tcgcgctcta ccccgacaat ctggtgcggt tcaacgccga
     1621 ccccttcgtc atggtgccga tctgcttggc catcgtgctg ggtggcctcg gcttcccggt
     1681 ctacctcgag gtcgtcgagc gcgtgcgcgg gcacctgccc cccttctggt cggtgcacct
     1741 gcgcctgacc ctgtacggca cggtgatcct cctcgtgctc gggttcgcga ccttcgccgc
     1801 tttcgagtgg accaacccgg ccaccctcgg cggtcaaggt ccgatgggga agttcctggg
     1861 tgcgctgggt gggtcggtgt tcccgcgcac ggctgggttc aactcggtcg actatgcctt
     1921 ggtcaccgac gagaccctcg cggtcgactt cggcctgatg atgatcggtg ggggttccgg
     1981 cggcacggcg ggcggcctga aggtcaccac ggtcgccgtg ctcctgctcg ccgtcatcac
     2041 cgaggtgcgc ggccatgaga agacggtcct cggcagccgc aagatctcct ctgctgtgtc
     2101 ccgtcaagcg ctggcagtcg tcgtgctggg cacagcggcg gtgttcgtcg gcatcctcgc
     2161 gatctccgcg gtcagcgacg cgcccctgcg cgaggacacg ttcgaggcga tctcggcgtt
     2221 cggcaccgtc ggcctgtcca tgaacctgac ccccaccctg ccccccgcgg cgtggggcgt
     2281 cctgatggga ctgatgtact tgggtcgcgt cggaccggtg tccgtcgcgg ccgccctggc
     2341 gatgaccgcc cggcaccgcc actacgaatt gccgagggag gacccccttg ttggataagt
     2401 tgttccggcc ccgccccgcc gacggcatgg tgctcgtgct cggcctgggg cgtttcggca
     2461 tcgcctgcgc gcgccgcctc caggaactcg gcatggacgt gctcggcgtg gacgccgacc
     2521 tcgacctggt caaccgctac gccgacacgc tgcggcacgt cgtccggatg gactccaccg
     2581 acgcggacgc cctgcgccag cttggcctgg agaacgtcgg gatcgcgatc gtgtcgatgg
     2641 cgcacctgga gtcgtccatc atcacgctct tgaccctgaa ggaggagggt gtccccgagg
     2701 tctgggccaa ggcgtcctcc cgcaagcacg gggagctcct gaagcggatc ggcgccgacc
     2761 acatcgtcta ccccgagagg tcggcggggg agcgcgtggc acacaccgtc gccggccaca
     2821 tgatcgacta cttcgagttc gaggacggct tcgccatggc ccggacgctg gcgccttcct
     2881 tcgcgtgggg ccgttccctg gccgagtcgg cgatccgcac tcgccacgac gtcacggtcg
     2941 tcggcctcaa gcggcccggg gaggacttca tctacgccgt gcccgagacc gtcgtccttg
     3001 agggcgacca attgatcgtg tccggcagca agcgcgacgt cgaacggttc gcccgcatca
     3061 gttgacgcaa ccccgaatgg gaatctggga acgtctgccc caagacggtg cactaccacg
     3121 agagccctat gtcaagcagc ctcttgttgg agggtgttga gccggccgtg taggtcgggg
     3181 ttgtagggct ggtggtcgtg ccagcagcgc cacaggatcc tggtccagcc ttgggccagg
     3241 atccttgctg cgtgtggatg tcggcagccg cggttgcgtg ctcggtcgta gggcgtgttg
     3301 ctaaaccctg ttcgtagcca gtcgagtgtg gcggctaggc agatgccggc gtggtagttg
     3361 cgggcgtact tgtcagatct catcgccagg ccgcgccact gcttgagctt gttgaagcag
     3421 cgctcgacga cgttgcggcc cttgtagcgt tcgcgttgtg cctcgccgaa gtcgatcggt
     3481 ctgccgggct tcttacgccg gtgggcgatt tggtcgtccc gctcgggaat cgtggtggcg
     3541 atgccgtgcc ggcgtagcca ggtgcggttc gctttggagg ggtagccctt gtcggccagc
     3601 agtcgatccg gtcgggtccg cgcacggccg ctggtgggca cccgaacctg ctccatcgtc
     3661 tggatcaaca tgctcgtgtc ggcgctctgg ccgggcgtga ggatgaacgc cagcactcgc
     3721 ccccgaccgt cgctgaccag gtgattcttg gtcgtcaggc ccccacgaga gcgtccgat
//
LOCUS       DAOSUG010000296         3775 bp    DNA     linear   ENV 10-JUN-2024
DEFINITION  MAG TPA_asm: Micropruina sp. isolate AMR_MDS_2195
            NODE_10066_length_3775_cov_3.429032, whole genome shotgun sequence.
ACCESSION   DAOSUG010000296 DAOSUG010000000
VERSION     DAOSUG010000296.1
DBLINK      BioProject: PRJNA925477
            BioSample: SAMN32864262
            Sequence Read Archive: SRR4236673
KEYWORDS    WGS; ENV; Metagenome Assembled Genome; MAG; Third Party Data; TPA;
            TPA:assembly.
SOURCE      Micropruina sp. (wastewater metagenome)
  ORGANISM  Micropruina sp.
            Bacteria; Bacillati; Actinomycetota; Actinomycetes;
            Propionibacteriales; Nocardioidaceae; Micropruina.
REFERENCE   1  (bases 1 to 3775)
  AUTHORS   Abdulkadir,N., Saraiva,J.P., Zhang,J., Stolte,S., Gillor,O.,
            Harms,H. and Rocha,U.
  TITLE     Genome-centric analyses of 165 metagenomes show that mobile genetic
            elements are crucial for the transmission of antimicrobial
            resistance genes to pathogens in activated sludge and wastewater
  JOURNAL   Microbiol Spectr 12 (3), e0291823 (2024)
   PUBMED   38289113
REFERENCE   2  (bases 1 to 3775)
  AUTHORS   Abdulkadir,N.
  TITLE     Direct Submission
  JOURNAL   Submitted (24-MAR-2023) Environmental Microbiology, Helmholtz
            Center for Environmental Research -UFZ, Permoserstr 15, Leipzig
            04318, Germany
COMMENT     The annotation was added by the NCBI Prokaryotic Genome Annotation
            Pipeline (PGAP). Information about PGAP can be found here:
            https://www.ncbi.nlm.nih.gov/genome/annotation_prok/
            
            ##Genome-Assembly-Data-START##
            Assembly Method        :: SPAdes v. 3.11.1
            Genome Representation  :: Full
            Expected Final Version :: Yes
            Genome Coverage        :: Not Applicable
            Sequencing Technology  :: Illumina MiSeq
            ##Genome-Assembly-Data-END##
            
            ##Genome-Annotation-Data-START##
            Annotation Provider               :: NCBI
            Annotation Date                   :: 05/04/2023 04:57:10
            Annotation Pipeline               :: NCBI Prokaryotic Genome
                                                 Annotation Pipeline (PGAP)
            Annotation Method                 :: Best-placed reference protein
                                                 set; GeneMarkS-2+
            Annotation Software revision      :: 6.5
            Features Annotated                :: Gene; CDS; rRNA; tRNA; ncRNA
            Genes (total)                     :: 2,776
            CDSs (total)                      :: 2,750
            Genes (coding)                    :: 2,714
            CDSs (with protein)               :: 2,714
            Genes (RNA)                       :: 26
            tRNAs                             :: 24
            ncRNAs                            :: 2
            Pseudo Genes (total)              :: 36
            CDSs (without protein)            :: 36
            Pseudo Genes (ambiguous residues) :: 0 of 36
            Pseudo Genes (frameshifted)       :: 2 of 36
            Pseudo Genes (incomplete)         :: 32 of 36
            Pseudo Genes (internal stop)      :: 2 of 36
            ##Genome-Annotation-Data-END##
FEATURES             Location/Qualifiers
     source          1..3775
                     /organism="Micropruina sp."
                     /mol_type="genomic DNA"
                     /submitter_seqid="NODE_10066_length_3775_cov_3.429032"
                     /isolate="AMR_MDS_2195"
                     /isolation_source="Activated sludge of municipal
                     wastewater treatment plants"
                     /db_xref="taxon:2737536"
                     /environmental_sample
                     /geo_loc_name="Canada"
                     /lat_lon="49.25 N 123.1 W"
                     /collection_date="2015-06-16"
                     /metagenome_source="wastewater metagenome"
                     /note="metagenomic"
     gene            294..623
                     /locus_tag="PLF56_09815"
     CDS             294..623
                     /locus_tag="PLF56_09815"
                     /inference="COORDINATES: protein motif:HMM:NF016854.2"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="TfoX/Sxy family protein"
                     /protein_id="HOQ53897.1"
                     /translation="MAFDEILAGQVRTLLVDESDVTEKRMFGGLAFLVAGHMAVAVSG
                     GGGLMVRTAPSEAQAPYFAHAEPMVMNGRELAGWVLMPAEPLTDEATLAAVVGHARAF
                     VRTLPAK"
     gene            644..2044
                     /gene="fumC"
                     /locus_tag="PLF56_09820"
     CDS             644..2044
                     /gene="fumC"
                     /locus_tag="PLF56_09820"
                     /EC_number="4.2.1.2"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_002515745.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_function: GO:0004333 - fumarate hydratase activity
                     [Evidence IEA];
                     GO_process: GO:0006099 - tricarboxylic acid cycle
                     [Evidence IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="class II fumarate hydratase"
                     /protein_id="HOQ53898.1"
                     /translation="MMSTRIESDSMGTIEVQSDRYWGAQTQRSLGNFDIGRDTFVWGR
                     PMIRALGVLKKAAAQANADLAQLPPDVAELIVQAADEVIAGKLDDHFPLVVFQTGSGT
                     QSNMNSNEVISNRGIELAGGELGSKKPVHPNDHVNRGQSSNDTFPTAMHIAVVCELNE
                     RLYGPVTELRDVLAAKAAEFDDVIMVGRTHLQDATPIRLGQVIGSWVAQIDFALEGVR
                     AADARARGLAIGGTAVGTGLNAHPDFGALTAQKISDETGLEFHQAGNLFAALSAHDSL
                     VEVSGSLRVLAGALMKIANDVRWYASGPRNGIGELLIPENEPGSSIMPGKVNPTQCEA
                     LTMVATRVFGNDVTVGFAGSQGNFQLNVYKPVMAHAVLESTRLLADGCRSFTEHCAVG
                     IEPNRAKIDANLSSNLMVVTALNRHIGYDKAAAMAKKAHKQGITLREAALASGDLTGE
                     EFDAWVVPADMTHPSA"
     gene            complement(2063..3457)
                     /locus_tag="PLF56_09825"
     CDS             complement(2063..3457)
                     /locus_tag="PLF56_09825"
                     /inference="COORDINATES: ab initio
                     prediction:GeneMarkS-2+"
                     /note="Derived by automated computational analysis using
                     gene prediction method: GeneMarkS-2+."
                     /codon_start=1
                     /transl_table=11
                     /product="hypothetical protein"
                     /protein_id="HOQ53899.1"
                     /translation="MYESLIDSPRDAIGRLRRSMQLRRQADIDEMQALSELAAEHSWS
                     TEDELDVTRERATRLGADGSPLVGEFLPLEVAAVKGISTTAATWLIRDVINLQSRHPH
                     LWGWVCRGEVQPYRAFQLVQLAAKYELTEEQAHDLDTALAPKLGTLGWARLMRLARGL
                     IALVATDAVRAAADRARAERFCRIAPSDEPVVSDVWARLDTTDAQQLDATLSALAKAM
                     AGLGDTDDLDVRRARALGILATPQRATALLAGDDDERYLPRCRVYLHLSHDMLPELVA
                     AADAPEPDACAASHTSGPTACAHSRPRSSVARSEIMGPIVKAQLAQLFATHRITVTPV
                     VHAGEEPAVDSYEIPQRIAEAVRLRDVCEVFPYSSRVARRLDLDHTAAYVPDVPQQTR
                     PDNLGPLTRRVHRAKTARQWQLRQPASGVFWWRSPRGQTYRVTPQGTTDLHHWSVGEC
                     ALRWRLDRRPPPTQ"
BASE COUNT          637 a         1180 c         1292 g          666 t
ORIGIN      
        1 ctggcagctt cccgatgtcg tactccgact ctgcacaact ccggatcggt gaattacagg
       61 cgctcaatcg ttgatacgtc acgtggtacg caattgtgcg catgtccaca cagatcacgg
      121 ttgctgcaac cagggatagg gtctgcgtga cccagggggg ttgtgcgcga cgggggaaag
      181 cgcaggtggg agggttggcc gcgagggtgt acgggaggat agcccggtgc gcactcgcgg
      241 ccttccgcac tcgtgggcct cagtgccatt ccctgcccaa ctaggctgca gctgtggcat
      301 tcgatgaaat cctggccggc caggtccgca ccctgttggt cgacgaatcg gacgtcaccg
      361 agaagcgaat gttcggcggg ttggccttcc tggtggcggg tcacatggct gtcgcggtgt
      421 cgggtggtgg gggactgatg gtgcgcaccg caccgtccga agctcaggcc ccgtactttg
      481 cccatgccga gccgatggtg atgaatggcc gcgagttggc gggctgggta ctgatgccgg
      541 ccgagccgct caccgatgag gcgacgctcg ctgcggtggt cggccatgcg cgggcgttcg
      601 ttcgaaccct gcccgcgaag taggtttcgg caatattctc ggcatcatga gcacgcgcat
      661 cgaatccgac agcatgggca ccatcgaggt gcagtccgac cgctactggg gggctcagac
      721 acagcgcagc ctcggcaact tcgacatcgg gcgcgacacg ttcgtgtggg ggcgtccgat
      781 gatccgggcc ctgggggtgc tcaagaaggc ggccgcgcag gcgaacgccg acctcgccca
      841 gttgccgccc gacgtggctg agctcatcgt gcaggcggcc gacgaagtga tcgccggcaa
      901 gcttgatgac cactttccgt tggtggtgtt tcagaccggg tcgggcaccc agtcgaacat
      961 gaactccaac gaggtgatca gcaatcgcgg catcgagttg gccgggggtg agttgggcag
     1021 caagaagcct gtgcacccca acgaccacgt gaaccgcggg cagtcgtcca acgacacctt
     1081 tcccaccgcg atgcatatcg cggtggtgtg cgagttgaac gagcggctgt acggtcccgt
     1141 cactgagttg cgcgatgtgc tggccgcgaa ggccgccgag ttcgacgacg tgatcatggt
     1201 cggccgcacc cacctgcagg acgccactcc gatccggctc ggtcaggtga tcggcagctg
     1261 ggtcgcgcag atcgatttcg ccctggaggg tgtgcgcgcg gccgatgcgc gcgcccgcgg
     1321 tctggcgatc ggcggcacgg ccgtgggcac cggcctcaat gcacaccccg acttcggtgc
     1381 gttgaccgcg cagaagatct cggacgagac cgggcttgag ttccatcagg ccggcaacct
     1441 gttcgccgcc ttgtccgccc acgattcatt ggttgaggtg agcggctcgc tgcgggtgct
     1501 cgccggcgcc ctgatgaaga tcgcgaacga cgtgcgttgg tacgcctccg ggccccgcaa
     1561 cggcatcggt gagttgttga ttcccgagaa cgagccaggt tcgtccatca tgccgggcaa
     1621 ggtgaacccc acccagtgtg aagcgctgac catggtggcg acccgagtgt tcggcaacga
     1681 tgtcaccgtc ggtttcgccg gtagtcaggg caacttccag ctcaatgtct acaagccggt
     1741 gatggcgcat gcggtgctcg agtcgactcg gctgctggct gacggctgcc gctcgttcac
     1801 cgagcactgc gcggtgggca ttgagccgaa ccgggccaag atcgacgcga acctgagcag
     1861 caacctgatg gtggtcacag cgctcaaccg gcatatcggc tacgacaagg cagccgccat
     1921 ggcgaagaag gcgcacaagc agggcatcac tcttcgtgaa gcagcccttg cctccggcga
     1981 cctgaccggc gaggagttcg acgcctgggt ggttccggcc gacatgacgc atccgtcggc
     2041 ctgatcagac ccgatcgtcc gatcattggg tgggcggtgg gcgtcgatcg aggcgccacc
     2101 gcagcgcgca ttcgcccacg gaccaatggt gcagatcagt ggtgccctgc ggggtgaccc
     2161 ggtacgtctg cccgcgcggt gagcgccacc agaacacacc gctcgcaggc tgacgaagtt
     2221 gccattggcg tgcggtcttg gctcggtgga ctcggcgggt gagtggcccc aggttgtcgg
     2281 ggcgggtctg ctgcgggacg tccggaacgt acgcggcggt atggtccagg tcgagccggc
     2341 gggccactcg actgctgtag ggaaagacct cgcacacatc gcgtagccgc actgcctcgg
     2401 cgatgcgctg cgggatctcg tagctgtcga cggcaggctc ttcgccggcg tgtaccacgg
     2461 gtgtcaccgt gatgcggtgg gtggcgaaca gctgggcaag ctgtgctttg acgatcgggc
     2521 ccatgatctc ggagcgggcg acactcgagc gggggcgcga gtgtgcgcag gcagtgggtc
     2581 cactggtatg cgaggcagcg caggcgtcag gttcaggtgc atctgcggcg gccaccagct
     2641 caggaagcat gtcgtgggac aggtgaaggt acacccggca gcgtggcaga taacgctcat
     2701 cgtcgtcgcc ggcaagcagc gcggtggcac gctgcggggt ggcaagtatg ccgagagccc
     2761 tggcccgccg cacgtcgagg tcgtcggtat cgccgaggcc ggccatggct ttggccagag
     2821 cgctcaaggt ggcgtcgagt tgctgggcgt cggtggtgtc gagccgagcc cacacgtcac
     2881 tcacgacggg ttcatcggac ggtgcgatgc gacagaaccg ctcagctcgc gctcgatcgg
     2941 cggcggctct gacggcatcg gtggcgacaa gagcgataag tccacgggcc agacgcatca
     3001 gccgagccca gcccaaggtg cccagcttcg gcgccaacgc ggtgtcgaga tcgtgagcct
     3061 gctcttcagt gagttcgtac ttcgcggcca gttggacaag ctggaacgct cggtagggct
     3121 gcacctcgcc gcggcacacc cagccccaca gatgcgggtg ccggctctgc aggttgatca
     3181 catcgcgaat gagccacgtg gctgcagtgg tggagatgcc tttcaccgca gccacctcca
     3241 gcggcagaaa ctcgccgacc agtggactgc catcggcgcc gaggcgggtc gcgcgttcgc
     3301 gggtgacgtc gagctcgtct tcggtgctcc aggaatgctc ggcggctaac tctgacagtg
     3361 cctgcatctc gtcgatgtcg gcttgacgac gtagctgcat cgagcgccgc aggcgcccaa
     3421 tggcatcacg cggagagtcg atgagcgatt cgtacatgtg tattattata ctcacacacg
     3481 cacgcctcca caagaccccg agcagactca tcctgattgc cggcggtgac gtttgtgata
     3541 cctctgacga agccatggat ctgggcctac tccggcatcc agttctgaat ctggcaggct
     3601 gagccggtgc ccgacgagac ctccagccag cccgtcgcgc catcgcctcc ggtgagtccc
     3661 gtcaccctgg tcaccccgac gcccctgacc ctgcgcgctc gatcgttctc ggcagcccgt
     3721 ccggccgtca tgggtatcgt gaactgcacg cccgactcgt tctatgccgc cgcac
//
LOCUS       DAOSUG010000297         3773 bp    DNA     linear   ENV 10-JUN-2024
DEFINITION  MAG TPA_asm: Micropruina sp. isolate AMR_MDS_2195
            NODE_10072_length_3773_cov_4.272996, whole genome shotgun sequence.
ACCESSION   DAOSUG010000297 DAOSUG010000000
VERSION     DAOSUG010000297.1
DBLINK      BioProject: PRJNA925477
            BioSample: SAMN32864262
            Sequence Read Archive: SRR4236673
KEYWORDS    WGS; ENV; Metagenome Assembled Genome; MAG; Third Party Data; TPA;
            TPA:assembly.
SOURCE      Micropruina sp. (wastewater metagenome)
  ORGANISM  Micropruina sp.
            Bacteria; Bacillati; Actinomycetota; Actinomycetes;
            Propionibacteriales; Nocardioidaceae; Micropruina.
REFERENCE   1  (bases 1 to 3773)
  AUTHORS   Abdulkadir,N., Saraiva,J.P., Zhang,J., Stolte,S., Gillor,O.,
            Harms,H. and Rocha,U.
  TITLE     Genome-centric analyses of 165 metagenomes show that mobile genetic
            elements are crucial for the transmission of antimicrobial
            resistance genes to pathogens in activated sludge and wastewater
  JOURNAL   Microbiol Spectr 12 (3), e0291823 (2024)
   PUBMED   38289113
REFERENCE   2  (bases 1 to 3773)
  AUTHORS   Abdulkadir,N.
  TITLE     Direct Submission
  JOURNAL   Submitted (24-MAR-2023) Environmental Microbiology, Helmholtz
            Center for Environmental Research -UFZ, Permoserstr 15, Leipzig
            04318, Germany
COMMENT     The annotation was added by the NCBI Prokaryotic Genome Annotation
            Pipeline (PGAP). Information about PGAP can be found here:
            https://www.ncbi.nlm.nih.gov/genome/annotation_prok/
            
            ##Genome-Assembly-Data-START##
            Assembly Method        :: SPAdes v. 3.11.1
            Genome Representation  :: Full
            Expected Final Version :: Yes
            Genome Coverage        :: Not Applicable
            Sequencing Technology  :: Illumina MiSeq
            ##Genome-Assembly-Data-END##
            
            ##Genome-Annotation-Data-START##
            Annotation Provider               :: NCBI
            Annotation Date                   :: 05/04/2023 04:57:10
            Annotation Pipeline               :: NCBI Prokaryotic Genome
                                                 Annotation Pipeline (PGAP)
            Annotation Method                 :: Best-placed reference protein
                                                 set; GeneMarkS-2+
            Annotation Software revision      :: 6.5
            Features Annotated                :: Gene; CDS; rRNA; tRNA; ncRNA
            Genes (total)                     :: 2,776
            CDSs (total)                      :: 2,750
            Genes (coding)                    :: 2,714
            CDSs (with protein)               :: 2,714
            Genes (RNA)                       :: 26
            tRNAs                             :: 24
            ncRNAs                            :: 2
            Pseudo Genes (total)              :: 36
            CDSs (without protein)            :: 36
            Pseudo Genes (ambiguous residues) :: 0 of 36
            Pseudo Genes (frameshifted)       :: 2 of 36
            Pseudo Genes (incomplete)         :: 32 of 36
            Pseudo Genes (internal stop)      :: 2 of 36
            ##Genome-Annotation-Data-END##
FEATURES             Location/Qualifiers
     source          1..3773
                     /organism="Micropruina sp."
                     /mol_type="genomic DNA"
                     /submitter_seqid="NODE_10072_length_3773_cov_4.272996"
                     /isolate="AMR_MDS_2195"
                     /isolation_source="Activated sludge of municipal
                     wastewater treatment plants"
                     /db_xref="taxon:2737536"
                     /environmental_sample
                     /geo_loc_name="Canada"
                     /lat_lon="49.25 N 123.1 W"
                     /collection_date="2015-06-16"
                     /metagenome_source="wastewater metagenome"
                     /note="metagenomic"
     gene            <1..246
                     /locus_tag="PLF56_09830"
     CDS             <1..246
                     /locus_tag="PLF56_09830"
                     /inference="COORDINATES: ab initio
                     prediction:GeneMarkS-2+"
                     /note="Derived by automated computational analysis using
                     gene prediction method: GeneMarkS-2+."
                     /codon_start=1
                     /transl_table=11
                     /product="hypothetical protein"
                     /protein_id="HOQ53900.1"
                     /translation="VGSVRATSSAVRSCSSLPAGFLLATGFTPAERAGRWRLDLGATA
                     RVPFEGVMDRLERLVQSVRPVAPPEPAGRQVIGAWRS"
     gene            complement(408..731)
                     /gene="trxA"
                     /locus_tag="PLF56_09835"
     CDS             complement(408..731)
                     /gene="trxA"
                     /locus_tag="PLF56_09835"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_013866150.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_component: GO:0005737 - cytoplasm [Evidence IEA];
                     GO_function: GO:0015036 - disulfide oxidoreductase
                     activity [Evidence IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="thioredoxin"
                     /protein_id="HOQ53901.1"
                     /translation="MAEVSAVTDDTFESLVVRSPKPVLVDYWADWCAPCRQISPIIDE
                     LAGEFGDRMGFYKLDTNTNPRIPTEQGVLGLPTIQIFSGGEVVKSFTGGKTKAALIRA
                     IEEFI"
     gene            complement(775..1752)
                     /gene="trxB"
                     /locus_tag="PLF56_09840"
     CDS             complement(775..1752)
                     /gene="trxB"
                     /locus_tag="PLF56_09840"
                     /EC_number="1.8.1.9"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_013866149.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_function: GO:0004791 - thioredoxin-disulfide reductase
                     activity [Evidence IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="thioredoxin-disulfide reductase"
                     /protein_id="HOQ53902.1"
                     /translation="MSEVREVIIIGSGPSGYTAAIYAARADLRPLVFEGALESGGALM
                     NTTDVENFPGFPEGIMGPDLMGNLRSQAERFGAEIVTDDIVEVDLTGVIKSVTDSEGG
                     VHRAKAVILAMGSGYRRLGVPGEDELSGRGVSWCATCDGFFFRDKDIAVVGGGDSALE
                     EATFLSRFASSVTVVHRRDELRASKIMIARAELDPKISFAWNCRVVSLNGNPSLTSVT
                     LADGISGEQRELEVQGCFIAIGHDPRSALVQGQVDTDAAGYVLVQPGTTHTNLPGVFA
                     AGDLVDHTYRQAITAAGTGCSAALDAERYLAELTDLAELAELSELSANV"
     gene            complement(1846..2910)
                     /locus_tag="PLF56_09845"
     CDS             complement(1846..2910)
                     /locus_tag="PLF56_09845"
                     /inference="COORDINATES: protein motif:HMM:NF019350.2"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_component: GO:0016020 - membrane [Evidence IEA];
                     GO_function: GO:0000155 - phosphorelay sensor kinase
                     activity [Evidence IEA];
                     GO_function: GO:0046983 - protein dimerization activity
                     [Evidence IEA];
                     GO_process: GO:0000160 - phosphorelay signal transduction
                     system [Evidence IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="histidine kinase"
                     /protein_id="HOQ53903.1"
                     /translation="MSHLPIEAILAALLLCTALVTSSEESGWAKAFNVVACVAGGLAR
                     TRPVFAAGLAALALVLLLATGTVPGIGLLGVHLVVLSWAAQGRRGTVPLALALLMLSL
                     PPVALWPENGRHTLTSVVFFTVLMAGTVLLGVTWRVLELRLIAAERARAEAVRDVRRS
                     IAQDLHDTVAQTDTLIAMRADGALEEPDVPPQVEAALREIAELARDSIQDLRAMLHVL
                     RAETDRPAWQVTDLGEFLDAQRDRLQRSGFPVDLQYQPVPASLRPALRQAVAKFLVEG
                     VNNIVRHATPGGRCLVALTHDAEQVQVTMVNDAEPGRAINRDGLGLMGARERIEALGG
                     QVAISTADGRWSLRAALPIA"
     gene            complement(3002..3166)
                     /locus_tag="PLF56_09850"
     CDS             complement(3002..3166)
                     /locus_tag="PLF56_09850"
                     /inference="COORDINATES: ab initio
                     prediction:GeneMarkS-2+"
                     /note="Derived by automated computational analysis using
                     gene prediction method: GeneMarkS-2+."
                     /codon_start=1
                     /transl_table=11
                     /product="hypothetical protein"
                     /protein_id="HOQ53904.1"
                     /translation="MRTFVIAIAFGLALAIGLTAGVPLGRPSVPAASTGSSTHVIPWL
                     CRIFGLCCER"
     gene            3407..3649
                     /locus_tag="PLF56_09855"
     CDS             3407..3649
                     /locus_tag="PLF56_09855"
                     /inference="COORDINATES: ab initio
                     prediction:GeneMarkS-2+"
                     /note="Derived by automated computational analysis using
                     gene prediction method: GeneMarkS-2+."
                     /codon_start=1
                     /transl_table=11
                     /product="hypothetical protein"
                     /protein_id="HOQ53905.1"
                     /translation="MLETYVRTRLAVEDFVRRQADVATRSEYRWVWLVLIGILVIAAA
                     VAWVYCRNAGYRGFSGHIEAVKGPFGIKIGIKLGCY"
BASE COUNT          598 a         1233 c         1343 g          599 t
ORIGIN      
        1 gtgggctctg ttcgagcgac gtcgtcagcg gttcggagct gttcgagcct gcccgcgggc
       61 tttctgctgg ccaccgggtt cacgcccgct gagcgtgccg gacggtggcg cctcgacctg
      121 ggcgccaccg cacgcgtgcc cttcgaaggc gtgatggatc gcttggaacg cctggttcag
      181 tcggttcggc cggtggcccc gcccgaaccg gcgggtcgtc aggtcatcgg cgcctggcgc
      241 agctgagcaa tgccgagtgc agtggttgat acgggctgac taggtgtttc gatgagcgca
      301 ccaccagttc agcgggggcg ttcggcgggc tcaacccacg agtctcggcg tggatctcgc
      361 aagagctcgg tcagccacgg gtcatgaccc ggccgaccag cgctgcgtca gatgaactcc
      421 tcgatggcgc gaatcagcgc ggccttggtc ttgccgccgg tgaacgactt gaccacttcg
      481 ccgccggaga agatctggat cgtcggtagg cccagcacgc cctgctcggt aggaattcgc
      541 gggttggtgt tggtgtcgag cttgtagaag cccatgcggt cgccgaactc gccggccaac
      601 tcgtcgatga tcggcgagat ctggcggcag ggggcgcacc agtcggccca gtagtcgacc
      661 agcacgggct tgggcgaccg caccaccagc gattcgaacg tgtcgtcggt gacagcgctg
      721 acttcggcca tcgggggatc ctctcggttg gtttcggggt gttgcgggac gaagtcagac
      781 gttagcgctg agctcggaca gttcggccaa ctcggcgagg tcggtcagtt cggccaggta
      841 gcgctcggcg tccagggccg ctgagcaacc cgtaccggcg gcggtgatcg cctgacggta
      901 ggtgtggtcg accaggtcac cggcggcgaa gacgcccggc aggttggtat gcgtggtgcc
      961 cggctgcacc agtacgtagc cggcggcatc ggtgtcgacc tggccctgaa ccagggccga
     1021 gcggggatcg tggccgatcg cgatgaagca gccctgcacc tcgagttcgc gctgctcgcc
     1081 gctgatgccg tcggccagcg tgaccgaggt gagcgacggg ttgccgttca gcgacaccac
     1141 ccggcagttc caggcgaagc tgatcttggg gtcgagttcg gctcgcgcga tcatgatctt
     1201 ggacgcgcgc agctcgtcgc ggcgatgcac cacggtgacc gaactggcga accgggacag
     1261 gaacgtggcc tcctcgagcg ccgagtcgcc gccgcccacc accgcgatgt ccttatcgcg
     1321 gaagaagaag ccgtcgcagg tcgcgcacca gctgaccccg cggccgctga gttcgtcctc
     1381 gccgggcacg ccgaggcgac gatagcccga tcccatggcc aggatcacgg ccttggcccg
     1441 gtgcacgccg ccctcggagt cggtcaccga cttgatcacg ccggtgaggt cgacctcgac
     1501 gatatcgtcg gtgacgatct cggcgccgaa ccgttcggcc tggctgcgta ggttgcccat
     1561 caggtcgggg cccatgatgc cctcgggaaa gccgggaaag ttctccacat cggtggtgtt
     1621 catcagggca ccgcccgact cgagggcccc ctcgaacacc agcggacgaa ggtcggcacg
     1681 cgctgcgtag atggcggcgg tgtagccgga cggaccggac ccgatgatga tgacttcgcg
     1741 aacctcagac atgcagttcc ttgcgtgtgg tggacgaccc acagcctaac cggcggtggc
     1801 atctacccga gaaacgagga ccgcgacccg gctattcccg ccctgttacg cgatcggtag
     1861 ggcggcccga aggctccagc gaccatccgc agtcgagatc gccacctgac ctcccagcgc
     1921 ctcgatgcgt tcgcgggcgc ccatcaggcc cagcccgtcg cggttgatcg cccgacccgg
     1981 ttcggcgtcg ttgaccatgg tgacctgcac ctgctcggcg tcgtgggtga gggcgaccag
     2041 gcagcgtccg ccgggggtgg cgtgccggac gatgttgttg accccctcga ccaggaactt
     2101 ggcgaccgcc tggcgcagcg ccggacggag cgaggcgggc accggttggt actgcaggtc
     2161 gaccggaaag ccgctgcgct gcagccggtc gcgctgggcg tccagaaact cgccgagatc
     2221 ggtgacctgc caggcaggac gatcggtctc ggcccgcagc acgtgcagca tggcgcgcag
     2281 gtcctgaatg gaatcgcggg ccagttcggc aatctcgcgc agcgcggctt cgacctgcgg
     2341 cggcacgtcc ggctcctcca gcgccccatc agcccgcatg gcgatcaggg tgtcggtctg
     2401 ggccaccgtg tcgtgcaggt cctgcgcgat gctgcgccgc acgtctcgga cggcctcggc
     2461 ccgggcccgt tcggctgcga tcaggcgaag ctccagtacc cgccaggtca cacccagcag
     2521 cacggtgccg gccatcagca ccgtgaagaa gacgacgctg gtcagcgtgt gccgcccgtt
     2581 ctcgggccac agggccaccg gcggcaggct gagcatcagc agggccagcg ccaacggcac
     2641 cgtgccccga cggccctggg cagcccacga gagcaccacc agatgcacgc ccagcaggcc
     2701 gatgccgggt acggttccgg ttgccagcag cagtaccagg gccaacgccg ccaacccggc
     2761 ggcgaagacg ggacgggtgc gggcgaggcc gccggccacg caggcgacga cgttaaaggc
     2821 tttcgcccag ccggactcct cggagctggt gaccagggcg gtgcacagca gcagtgcagc
     2881 caggatcgcc tcgatcggaa ggtgactcaa caggcggttc agtcgcgctg ccgatggcgt
     2941 cgtgggtgcg gggggtgctg ccacagggcc aattgtgtca gcacttcggg ttggcgtccg
     3001 ctcaacgctc gcaacagagc ccgaagatcc ggcacagcca gggaatgacg tgggtcgaac
     3061 tgccggtgct ggccgccggc acgctcggcc ggccgagcgg cacgccggcg gtcagcccga
     3121 tggccagggc gagtccgaac gcgatcgcga tcacgaatgt gcgcatgagg tgctcctttg
     3181 ctcccgaggt ggtactgcgc tcacaagcta ggcagcggac gcgagccagg gcgggggccc
     3241 ccgtcggccg acacctttcg gagacgttcg gtgtctccca aagttgcatg ttgtccgccg
     3301 ggcattgtcg agcggccggt gtccggcctg aactggtcac ggtcgttggt gggcaacggc
     3361 cgagcggcgc cgaggcgacg atcgcccggc gcggagagga gtcatcatgt tggagaccta
     3421 cgtccggacg cggcttgccg tggaggactt cgtccgtcgg caagcggacg tggcgacccg
     3481 cagcgagtac cgctgggtgt ggctggtgct gatcggcatc ctggtgatcg ctgcggccgt
     3541 cgcctgggtc tactgccgca acgccggcta ccgcgggttc agcggtcaca tcgaggcggt
     3601 caagggtccg ttcggaatca agatcggcat caagctcggc tgctactgag cctcacgact
     3661 agtgctgaag gaggcgtggt ggatattccg ctcatggtgc gtggcctgac caagacctat
     3721 ggcacccgga ccggcatcgc cggggcgagc ttcgaggtga ggcccggcga gat
//
LOCUS       DAOSUG010000298         3772 bp    DNA     linear   ENV 10-JUN-2024
DEFINITION  MAG TPA_asm: Micropruina sp. isolate AMR_MDS_2195
            NODE_10075_length_3772_cov_3.167877, whole genome shotgun sequence.
ACCESSION   DAOSUG010000298 DAOSUG010000000
VERSION     DAOSUG010000298.1
DBLINK      BioProject: PRJNA925477
            BioSample: SAMN32864262
            Sequence Read Archive: SRR4236673
KEYWORDS    WGS; ENV; Metagenome Assembled Genome; MAG; Third Party Data; TPA;
            TPA:assembly.
SOURCE      Micropruina sp. (wastewater metagenome)
  ORGANISM  Micropruina sp.
            Bacteria; Bacillati; Actinomycetota; Actinomycetes;
            Propionibacteriales; Nocardioidaceae; Micropruina.
REFERENCE   1  (bases 1 to 3772)
  AUTHORS   Abdulkadir,N., Saraiva,J.P., Zhang,J., Stolte,S., Gillor,O.,
            Harms,H. and Rocha,U.
  TITLE     Genome-centric analyses of 165 metagenomes show that mobile genetic
            elements are crucial for the transmission of antimicrobial
            resistance genes to pathogens in activated sludge and wastewater
  JOURNAL   Microbiol Spectr 12 (3), e0291823 (2024)
   PUBMED   38289113
REFERENCE   2  (bases 1 to 3772)
  AUTHORS   Abdulkadir,N.
  TITLE     Direct Submission
  JOURNAL   Submitted (24-MAR-2023) Environmental Microbiology, Helmholtz
            Center for Environmental Research -UFZ, Permoserstr 15, Leipzig
            04318, Germany
COMMENT     The annotation was added by the NCBI Prokaryotic Genome Annotation
            Pipeline (PGAP). Information about PGAP can be found here:
            https://www.ncbi.nlm.nih.gov/genome/annotation_prok/
            
            ##Genome-Assembly-Data-START##
            Assembly Method        :: SPAdes v. 3.11.1
            Genome Representation  :: Full
            Expected Final Version :: Yes
            Genome Coverage        :: Not Applicable
            Sequencing Technology  :: Illumina MiSeq
            ##Genome-Assembly-Data-END##
            
            ##Genome-Annotation-Data-START##
            Annotation Provider               :: NCBI
            Annotation Date                   :: 05/04/2023 04:57:10
            Annotation Pipeline               :: NCBI Prokaryotic Genome
                                                 Annotation Pipeline (PGAP)
            Annotation Method                 :: Best-placed reference protein
                                                 set; GeneMarkS-2+
            Annotation Software revision      :: 6.5
            Features Annotated                :: Gene; CDS; rRNA; tRNA; ncRNA
            Genes (total)                     :: 2,776
            CDSs (total)                      :: 2,750
            Genes (coding)                    :: 2,714
            CDSs (with protein)               :: 2,714
            Genes (RNA)                       :: 26
            tRNAs                             :: 24
            ncRNAs                            :: 2
            Pseudo Genes (total)              :: 36
            CDSs (without protein)            :: 36
            Pseudo Genes (ambiguous residues) :: 0 of 36
            Pseudo Genes (frameshifted)       :: 2 of 36
            Pseudo Genes (incomplete)         :: 32 of 36
            Pseudo Genes (internal stop)      :: 2 of 36
            ##Genome-Annotation-Data-END##
FEATURES             Location/Qualifiers
     source          1..3772
                     /organism="Micropruina sp."
                     /mol_type="genomic DNA"
                     /submitter_seqid="NODE_10075_length_3772_cov_3.167877"
                     /isolate="AMR_MDS_2195"
                     /isolation_source="Activated sludge of municipal
                     wastewater treatment plants"
                     /db_xref="taxon:2737536"
                     /environmental_sample
                     /geo_loc_name="Canada"
                     /lat_lon="49.25 N 123.1 W"
                     /collection_date="2015-06-16"
                     /metagenome_source="wastewater metagenome"
                     /note="metagenomic"
     gene            <1..903
                     /locus_tag="PLF56_09860"
     CDS             <1..903
                     /locus_tag="PLF56_09860"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_011758041.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_function: GO:0022857 - transmembrane transporter
                     activity [Evidence IEA];
                     GO_process: GO:0055085 - transmembrane transport [Evidence
                     IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="MFS transporter"
                     /protein_id="HOQ53906.1"
                     /translation="NQPTRTAVLPALLPLDQLPSASSLNMTVMSAGAIAGPLVGGALI
                     PVFGFPLLYLIDTVCLLATLWAVTSLPKLPPLTAHRGTPGLRSVLDGLRYLLGHPILL
                     TSFLVDGIAMIFGMPRALFPQVAHQSFGGPSDGGLAFALLFAAMPAGAVLGGVVSGWV
                     SRIDRQGLAVVWAVAAWGAAIVVTGAAIWFAPSAPTAMLAVAVAALAFGGAADMASAA
                     FRQSMLLGAADDDVRGRLQGVFIVVVAGGPRIADVLHGWAADGIGTAATTAGGGVLVI
                     VGVAITAVALPAFVRYRLSEHPVR"
     gene            1036..2106
                     /locus_tag="PLF56_09865"
     CDS             1036..2106
                     /locus_tag="PLF56_09865"
                     /inference="COORDINATES: protein motif:HMM:NF024910.2"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_component: GO:0016020 - membrane [Evidence IEA];
                     GO_function: GO:0022857 - transmembrane transporter
                     activity [Evidence IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="APC family permease"
                     /protein_id="HOQ53907.1"
                     /translation="MTSTQLQDTLLPKRLALPVYCSDPISSNAYATQEILLVLALGGA
                     SMVLFTPWVAVAVVSLLALVTLSYRQTCHAYPDGGGAYAVSKANLGGNASLVAASALM
                     VDYVMTVAVSIASGVENLVSAIPALRPYSVMLCLVFIVLLTVMNLRGVRESGTLFAIP
                     TYGFLVSVFVMLGAGFFNVATGHAPVAESAGFGFVPTDPTGVALIILLLRAFASGCTS
                     LTGVEAVSNGVPTFRKPKAHNASLTLLAMGGLSIAMMIGISVLSTVSHIHIADSLGDL
                     TGVPAGYEQRTVLAQLSAAVFGNNTLGFFAVQGFTTAIRCWRPTPRSTAFRSSPRSSP
                     PTCTCPSSCGAGGTGWCSATAS"
     gene            3047..3538
                     /locus_tag="PLF56_09870"
     CDS             3047..3538
                     /locus_tag="PLF56_09870"
                     /inference="COORDINATES: protein motif:HMM:NF024884.2"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="DUF4118 domain-containing protein"
                     /protein_id="HOQ53908.1"
                     /translation="MALIGPLALTVLLLPLRDRLGVDTVLLLFVLVSVTASAVGGVLP
                     ALVASALGCGLANFFFTRPYGSLTVASTDELIDLLIFLAVAVVVGVVTEWGGRARARS
                     EGAKVRAEWLAGLDARSEEPDSVEVALGEARALFAMNRAALVDATGHELAAWVPAGPG
                     TRR"
     gene            3535..>3772
                     /locus_tag="PLF56_09875"
     CDS             3535..>3772
                     /locus_tag="PLF56_09875"
                     /inference="COORDINATES: ab initio
                     prediction:GeneMarkS-2+"
                     /note="Derived by automated computational analysis using
                     gene prediction method: GeneMarkS-2+."
                     /codon_start=1
                     /transl_table=11
                     /product="hypothetical protein"
                     /protein_id="HOQ53909.1"
                     /translation="MNADAGEGLRLALSGPARVGADRSLLRDLAATTARLWRARELTE
                     RARRAEELARIDELRSDLLAAVGHDLRNPLAALRA"
BASE COUNT          459 a         1310 c         1344 g          659 t
ORIGIN      
        1 aaccagccca cccgcaccgc cgtgctgccg gcgctgctgc cgctggatca gctgccgtcg
       61 gccagttcgc tgaacatgac cgtgatgagc gcgggcgcca tcgccgggcc gctggtcggc
      121 ggggcgctga ttccggtctt cggctttccc ctgctctacc tgatcgacac cgtctgcctg
      181 ctcgccacct tgtgggcggt gaccagcctg ccgaaactgc ctccgctcac ggcgcaccgc
      241 ggcacgcccg gcctgcgctc ggtgctggac ggcctgcgct acctgctcgg ccacccgatt
      301 ctactgacgt cgttcctggt ggacggcatc gcgatgatct tcggcatgcc gcgcgcactg
      361 ttcccccagg tggcgcacca gagcttcggc ggtccgtccg acggcggcct ggccttcgcg
      421 ctgctcttcg ccgccatgcc ggccggtgcc gtgctgggcg gagtggtgtc ggggtgggtg
      481 tcgcgcatcg accggcaggg cctggccgtg gtgtgggcgg tggccgcgtg gggtgcggcg
      541 atcgtcgtca ccggagccgc gatctggttc gcgccgtccg caccgacggc gatgctcgcg
      601 gtcgcggtcg ccgcgctggc cttcggtggt gccgccgaca tggcctcggc ggccttccgg
      661 cagtcgatgc tgctgggcgc tgccgacgac gacgtgcgcg gacggctgca gggcgtgttc
      721 atcgtggtcg tggcgggtgg accacggatc gcggacgtgc tgcacggctg ggccgcggac
      781 ggcatcggca cggctgccac caccgcgggc ggcggcgtgc tggtgatcgt cggcgtggcc
      841 atcaccgccg tcgccttgcc ggccttcgtc cgctaccggc tgtctgagca cccggtgcgg
      901 taagggaggc gtcaagaacc ggcggcgcgg cgtcaaggac gcatcaagac gcgagcgcgc
      961 accccgcgac cggagtgtgc tgggcacgtg accgtctcga tgcgtctgcc caagcgcctg
     1021 ctcgtcggta atccgatgac ctccacccaa ctgcaggaca cgctgctgcc gaagcggctc
     1081 gccctgcccg tgtactgctc cgacccgatc agctcgaacg cgtacgccac gcaggagatc
     1141 ctgctggtgc tcgctctcgg cggggcgtcc atggtgctgt tcacgccgtg ggtggcggtg
     1201 gcggtggtgt cgctgctggc gctggtcacg ctgagctacc ggcagacctg ccacgcctac
     1261 cccgacggtg gcggcgccta cgcggtcagc aaggccaacc tgggcggcaa tgcgtcgctg
     1321 gtcgccgcgt cggccctgat ggtggactac gtgatgacgg tcgcggtgtc gatcgcgtcc
     1381 ggggtcgaga acctggtgtc ggcgattccc gccctgcggc cctactcggt gatgctgtgc
     1441 ctggtgttca tcgtgctgct gaccgtgatg aacctgcgtg gggtgcgcga gtcgggcacc
     1501 ctgttcgcca ttccgaccta cggttttctg gtcagtgtgt tcgtgatgct cggggccggg
     1561 ttcttcaacg tggcgaccgg gcacgccccc gtcgccgagt cggccggttt cggcttcgtg
     1621 ccgaccgacc cgaccggtgt cgcactgatc atcctgctgc tgcgcgcctt cgcctccggc
     1681 tgtacctcgc tgaccggcgt cgaagccgtc agcaacgggg tgccgacctt tcgcaagccc
     1741 aaggcgcaca acgcgtcgct gaccctgctg gcgatgggcg ggctgagcat cgcgatgatg
     1801 atcggcatct cggtgctgag caccgtctcg cacattcaca tcgccgactc actcggcgac
     1861 ctgaccggcg tgcccgccgg ctacgagcag cggacggtgc tggcccagct gtcggcggcg
     1921 gtgttcggca acaacaccct cggcttcttc gctgtgcagg gcttcaccac ggcgatccgg
     1981 tgctggcggc caacaccgcg ttcaacggct ttccgatcct cgcctcgatc ctcgccgccg
     2041 acctgtacat gcccaagcag ctgcggcgcc ggggggaccg gctggtgttc agcaacggca
     2101 tcctgatcct ggcggcagtg gccggtggcc tgatcgtggt cttcgacgga tcggtgaccc
     2161 ggctgatcca gctgtacatc ctgggagtgt tcgtgtcgtt cacgctcagc cagctcggca
     2221 tggtggtgca ttggcgccgg gacgccgccg cccacggctg gagcacccgc aagctcggtg
     2281 cgctcagcat caacgccgtc ggcggtgtgg ccaccgcact ggtgctggtg atcgtgctga
     2341 tcaccaagtt cacccacggc gcctacctgg tggtgatcgc cattccggtg ctgttcttcg
     2401 tgatgaagcg gatccgccgc cactacgagc gggtgtcgaa acagcttcgt cccaaggccg
     2461 ccggcatcac gctgcccagc cgcacccacg ccgtcgtgct gatcagccaa ctcaacgagc
     2521 cggcgctgaa ggcgctcgcc ttcgctcgcg cgatccgtcc cgacacgatc accgcgctgc
     2581 gcgtcgacac cgaccccgaa cgaacctcga agctggtcga ggagtggacc ggccgcgaga
     2641 tcaaggtgcc gctgaccatc gtcagttctg tataccgcga tctcaccgca cccgtgctgg
     2701 actacctggc cgggttgcgc gtcggcccgc gcgacgtggt gcaggtgttc gtgcccgaat
     2761 acgtggtggg gcactggtgg gagcaggtgc tgcacaacca gagtgcgctg cggttgaagg
     2821 cccggctgct gtacatgccc ggcgtgatgg tgaccagcgt gccgtaccag ttgaagtcgg
     2881 ccgagcccta cattctggag cgtcctggcc tggaagccga tcacgaccgg cgcggtctgg
     2941 caagctcgcg cttgcgagtg agcggccgga ggatcgggtg ggtgattcgg tgccgcccgc
     3001 gcccccggcc gcactgccgc ggcccaggcg gctcgccggg catgcggtgg cactgatcgg
     3061 tccgctggcg ctcaccgtgt tgctgctgcc gctgcgcgac cggctgggtg tcgacacggt
     3121 gctgctgctg ttcgtgctgg tgtcggtgac cgcctcggcg gtcggcgggg tgttgcctgc
     3181 gctggtggcc tcggcgctgg ggtgcgggct ggcgaacttc ttcttcaccc gcccctacgg
     3241 cagcctcacc gtggccagca ccgacgaact catcgacctg ctgatctttc tggccgtcgc
     3301 ggtggtggtc ggcgtggtca ccgagtgggg tggacgcgcc cgggcccgat cagagggtgc
     3361 caaggtgcgc gccgagtggc tggccggcct cgacgcccgc agcgaagagc ccgactccgt
     3421 cgaggtggcc ctgggcgagg cccgcgccct gttcgcgatg aaccgggcgg ccctggtgga
     3481 cgccaccggg cacgagttgg ccgcgtgggt gccggccggg ccggggacgc gacgttgaac
     3541 gccgatgccg gcgagggcct gcggttggcc ctgagcgggc cggcgcgggt cggtgccgac
     3601 cggtccctgc tgcgtgacct ggcggccacc accgcccggc tgtggcgcgc tcgcgagctg
     3661 accgagcggg cccgcagagc cgaggaactc gcccggatcg atgagctgcg ctccgatctg
     3721 ctggccgccg tcggccacga cctgcgcaac ccgctggccg cgctgcgggc cg
//
LOCUS       DAOSUG010000299         3760 bp    DNA     linear   ENV 10-JUN-2024
DEFINITION  MAG TPA_asm: Micropruina sp. isolate AMR_MDS_2195
            NODE_10120_length_3760_cov_4.430229, whole genome shotgun sequence.
ACCESSION   DAOSUG010000299 DAOSUG010000000
VERSION     DAOSUG010000299.1
DBLINK      BioProject: PRJNA925477
            BioSample: SAMN32864262
            Sequence Read Archive: SRR4236673
KEYWORDS    WGS; ENV; Metagenome Assembled Genome; MAG; Third Party Data; TPA;
            TPA:assembly.
SOURCE      Micropruina sp. (wastewater metagenome)
  ORGANISM  Micropruina sp.
            Bacteria; Bacillati; Actinomycetota; Actinomycetes;
            Propionibacteriales; Nocardioidaceae; Micropruina.
REFERENCE   1  (bases 1 to 3760)
  AUTHORS   Abdulkadir,N., Saraiva,J.P., Zhang,J., Stolte,S., Gillor,O.,
            Harms,H. and Rocha,U.
  TITLE     Genome-centric analyses of 165 metagenomes show that mobile genetic
            elements are crucial for the transmission of antimicrobial
            resistance genes to pathogens in activated sludge and wastewater
  JOURNAL   Microbiol Spectr 12 (3), e0291823 (2024)
   PUBMED   38289113
REFERENCE   2  (bases 1 to 3760)
  AUTHORS   Abdulkadir,N.
  TITLE     Direct Submission
  JOURNAL   Submitted (24-MAR-2023) Environmental Microbiology, Helmholtz
            Center for Environmental Research -UFZ, Permoserstr 15, Leipzig
            04318, Germany
COMMENT     The annotation was added by the NCBI Prokaryotic Genome Annotation
            Pipeline (PGAP). Information about PGAP can be found here:
            https://www.ncbi.nlm.nih.gov/genome/annotation_prok/
            
            ##Genome-Assembly-Data-START##
            Assembly Method        :: SPAdes v. 3.11.1
            Genome Representation  :: Full
            Expected Final Version :: Yes
            Genome Coverage        :: Not Applicable
            Sequencing Technology  :: Illumina MiSeq
            ##Genome-Assembly-Data-END##
            
            ##Genome-Annotation-Data-START##
            Annotation Provider               :: NCBI
            Annotation Date                   :: 05/04/2023 04:57:10
            Annotation Pipeline               :: NCBI Prokaryotic Genome
                                                 Annotation Pipeline (PGAP)
            Annotation Method                 :: Best-placed reference protein
                                                 set; GeneMarkS-2+
            Annotation Software revision      :: 6.5
            Features Annotated                :: Gene; CDS; rRNA; tRNA; ncRNA
            Genes (total)                     :: 2,776
            CDSs (total)                      :: 2,750
            Genes (coding)                    :: 2,714
            CDSs (with protein)               :: 2,714
            Genes (RNA)                       :: 26
            tRNAs                             :: 24
            ncRNAs                            :: 2
            Pseudo Genes (total)              :: 36
            CDSs (without protein)            :: 36
            Pseudo Genes (ambiguous residues) :: 0 of 36
            Pseudo Genes (frameshifted)       :: 2 of 36
            Pseudo Genes (incomplete)         :: 32 of 36
            Pseudo Genes (internal stop)      :: 2 of 36
            ##Genome-Annotation-Data-END##
FEATURES             Location/Qualifiers
     source          1..3760
                     /organism="Micropruina sp."
                     /mol_type="genomic DNA"
                     /submitter_seqid="NODE_10120_length_3760_cov_4.430229"
                     /isolate="AMR_MDS_2195"
                     /isolation_source="Activated sludge of municipal
                     wastewater treatment plants"
                     /db_xref="taxon:2737536"
                     /environmental_sample
                     /geo_loc_name="Canada"
                     /lat_lon="49.25 N 123.1 W"
                     /collection_date="2015-06-16"
                     /metagenome_source="wastewater metagenome"
                     /note="metagenomic"
     gene            <1..1313
                     /locus_tag="PLF56_09880"
     CDS             <1..1313
                     /locus_tag="PLF56_09880"
                     /EC_number="6.3.4.2"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_017936229.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_function: GO:0003883 - CTP synthase activity [Evidence
                     IEA];
                     GO_process: GO:0006221 - pyrimidine nucleotide
                     biosynthetic process [Evidence IEA];
                     GO_process: GO:0006241 - CTP biosynthetic process
                     [Evidence IEA]"
                     /codon_start=3
                     /transl_table=11
                     /product="CTP synthase"
                     /protein_id="HOQ53910.1"
                     /translation="VIPHITNEIKDEMLSMAGRGIDVVIHEIGGTVGDIESLPFLEAA
                     RQVRRDVGRENVFFLHVSLVPYLGPSGELKTKPTQHSVAALRQVGITPDAIVCRSNRE
                     IPDGVKRKISLMCDVDEEAVVSCPDAPSIYDIPKVLHDEGLDAYVVRRLGVSFRDVNW
                     TKWNDLLQRVHHPKEDVTIGLVGKYIDLPDAYLSVSEALRAGGFANHAKVHIRWIASD
                     SCETPSGAAEQLGDLDGIVVPGGFGIRGVEGKLGALRYARENQLPTLGLCLGLQCMVI
                     EVARDLCGLDDAASTEFEPDTNAPVIATMAEQVAHVSGEGQLGATMRLGSYPADLTKG
                     SLVAKLYGTTHVAERHRHRYEVNNAYRDELEAAGLRVVGTSPDSKLVEFVELDPAQHP
                     FFVATQAHPELKSRPTKPHPLFVGLVGAALHRKLSARLPVEEDR"
     gene            1310..1999
                     /locus_tag="PLF56_09885"
     CDS             1310..1999
                     /locus_tag="PLF56_09885"
                     /EC_number="3.6.-.-"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_013161249.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_function: GO:0046872 - metal ion binding [Evidence
                     IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="NUDIX hydrolase"
                     /protein_id="HOQ53911.1"
                     /translation="MTSPLRVLGHGEGVDVPANWPLVAHRVLGEGAVSDYVDDIVDTP
                     AGERMHRQYLLHPGAVAVIALDEHDRVVVVRQYRHPVGFQLIEPPAGLLDADGESWLA
                     AAQRELAEEAMLAADDWRLLVDMFTSPGCNSESIRFFLARGLHAVPRPDGFVVEHEEL
                     DMELCLVPLTDLLEAIYAGRVQSPTIVAGVLALEAARLGGRLDDLRPADSPWPAREVR
                     HHLLDEIAKLA"
     gene            2007..2948
                     /gene="xerD"
                     /locus_tag="PLF56_09890"
     CDS             2007..2948
                     /gene="xerD"
                     /locus_tag="PLF56_09890"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_015070774.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_function: GO:0009009 - site-specific recombinase
                     activity [Evidence IEA];
                     GO_process: GO:0006310 - DNA recombination [Evidence IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="site-specific tyrosine recombinase XerD"
                     /protein_id="HOQ53912.1"
                     /translation="MPPQAAVQRVIGSFLDHLTVERGASGNTVAAYRRDLGRYQEFLD
                     ECGIAELTGVAEADVAAFAVTLREGRQGRPTLAPSSVARALSAVKSLHRFAVDDGLAA
                     DNPATGVSPPQAGRRLPKALSIDQVQALLDAPDVSTPGGLRDEALLELLYGTGARITE
                     LVSLDVDDVTHLTHDADAGLRLFGKGRKERVVPVGSYARAALDAWLVRGRPAMLEHAR
                     ASTPALFVNTLGRRLSRQSAWAMLRDCADRAGITAEIGPHTMRHSFATHLLDGGADVR
                     VVQELLGHASVTTTQIYTLVTVEHLREVYLTAHPRAR"
BASE COUNT          602 a         1281 c         1292 g          585 t
ORIGIN      
        1 aggtgattcc gcacatcacc aacgagatca aggacgagat gctgtcgatg gccggccggg
       61 gcatcgacgt ggtgatccac gagatcggcg gcaccgtcgg cgacatcgag tcgctgccct
      121 ttcttgaggc cgcccgccag gtgcgccgcg acgtcggacg cgagaacgta ttctttctgc
      181 acgtgtcgct ggtgccctac ctgggaccgt ccggcgagtt gaagaccaag ccgacccagc
      241 actcggtggc cgccctgcgc caggtcggca tcacccccga tgcgattgtc tgccgctcca
      301 accgtgagat tccggacggg gtcaagcgca agatctccct catgtgtgac gtggacgagg
      361 aagccgtggt gagttgcccc gacgcgccgt ccatctacga cattccgaag gtgttgcacg
      421 acgagggcct cgacgcctac gtggtgcggc ggctcggggt cagcttccgc gacgtgaact
      481 ggacgaagtg gaacgacctg ctgcagcgcg tccatcaccc caaggaggac gtcacgatcg
      541 gcctggtcgg caagtacatc gacctgccgg acgcctactt gagcgtatcc gaggcactgc
      601 gggcgggtgg tttcgccaac cacgccaagg tgcacattcg ctggatcgcc tccgactcat
      661 gcgagacccc gtccggggcg gccgaacaac tcggcgacct ggacggcatc gtggtgccgg
      721 gcggattcgg cattcgcggc gtcgagggca agctcggcgc gctcaggtac gcccgcgaga
      781 accagttgcc cacgctcggg ctgtgcctgg gcctgcagtg catggtgatc gaggtggccc
      841 gcgacctgtg cgggctggac gacgcggcgt ccacagagtt cgagccggac accaacgcgc
      901 cggtgatcgc gaccatggcc gaacaggtcg cgcacgtgtc gggggagggt cagttgggcg
      961 caacgatgcg gctcggctcg tacccggccg acctgaccaa gggttccctg gtcgcgaagc
     1021 tctacggcac cacgcacgtg gccgagcggc accggcaccg ctacgaggtc aacaacgcct
     1081 accgggacga actggaggcc gcgggactgc gggtggtcgg cacgtccccc gattcgaagc
     1141 tcgtcgagtt cgtcgaactc gacccggccc agcatccgtt cttcgtggcc acccaggccc
     1201 acccggaact gaagtcgcgg ccgaccaaac cccacccact gttcgtcggc ctggtcggcg
     1261 ccgcgctgca tcgcaagttg tcggcccggc tgccggttga ggaggaccga tgacctcccc
     1321 gctgcgcgtc cttgggcacg gcgagggcgt ggacgtgccg gcgaactggc cgttggtcgc
     1381 gcaccgggtg ctgggggagg gcgcagtcag cgactatgtg gacgacatcg tcgacactcc
     1441 ggccggtgaa cggatgcacc ggcagtacct gctgcacccc ggcgccgtcg cggtgatcgc
     1501 gctggacgag cacgaccgcg tggtggtggt gcggcagtac cggcacccgg tcggctttca
     1561 gctgatcgaa ccgccggcgg ggttgctcga tgctgacggt gaatcatggc tggcggccgc
     1621 gcagcgcgaa ctggccgaag aggcgatgct ggccgcggac gactggcggc tcctggtcga
     1681 catgttcacc tcgccggggt gcaactccga gtcgatccgg ttcttcctgg cccgggggct
     1741 gcacgccgtc ccccgtccgg acgggttcgt ggtcgagcac gaagaactcg acatggaact
     1801 gtgcctggtg ccattgaccg acctgctgga ggccatctac gccggccggg tgcagagccc
     1861 caccatcgtc gccggtgtgc tcgccctcga agcggcccgc ctcggtgggc ggctggacga
     1921 cctgcgcccc gccgacagcc catggccggc ccgcgaggtg cgtcaccacc tgctggacga
     1981 gatcgcgaag ctggcctgag cccgccatgc ccccacaggc ggccgtgcaa cgggtgatcg
     2041 ggtcgtttct cgatcacctg acggtggagc ggggggcgtc gggcaacacc gtcgccgcgt
     2101 accggcgcga tctgggccgc taccaggagt ttctggacga gtgcggaatc gcggaactga
     2161 ccggtgtggc cgaagcggac gtggcggcgt tcgccgtcac gctgcgcgag ggtcgccagg
     2221 gccggcccac actggcgccg agcagcgtcg cccgggccct gagcgcggtg aagtcgctgc
     2281 accgtttcgc cgtggatgac gggctggccg ccgacaaccc agcgacgggg gtgtcgcctc
     2341 cgcaggccgg gcgcaggctg cccaaggcgc tgtcgatcga tcaggtgcag gccctgctgg
     2401 acgctcccga cgtctctacc ccgggcggcc tgcgcgacga ggcgctgctg gagttgctgt
     2461 acggcaccgg cgcccggatc accgaactgg tctcgctcga cgtggacgac gtgacgcatc
     2521 tgacccacga tgcggatgcc ggcctgcgac tgttcggcaa gggccgcaag gaacgcgtgg
     2581 tgccggtcgg ctcgtacgcg cgcgccgccc tggacgcctg gctggtgcgg ggacgtccgg
     2641 ccatgctcga acacgcccgc gcctcgacgc cggcactctt cgtcaacacg ctgggtaggc
     2701 ggttgagccg gcagtccgcg tgggccatgc ttcgcgattg cgccgatcgg gccggcatca
     2761 ccgccgagat cggcccgcac accatgcggc actcgttcgc cacccacctg ctggacgggg
     2821 gagcggacgt ccgggtggtg caggaactgc tcggacacgc gtccgtcacc accacgcaga
     2881 tctacaccct ggtcaccgtc gagcacctgc gcgaggtgta tctgaccgcc catccccggg
     2941 cccgctgacg gcgaagcccg cacacgcctc ggcgtcctac gtgccgtcgt tggcaatcgg
     3001 gtgaggggcg aatcgaccag tcctgtccca cacctagagg gcacctcggt gatgcgggcg
     3061 acggacgctg gcgaagccgt gctgcgagtg cgccacctgg gcgaccgagc ggctgcgggc
     3121 cgacgctcac ctcggcagca acgtagcgat gccctcacac aggtcggtag gcaactggac
     3181 acgcttcacg actggcgtcc ataggcgaac gtcgcggccc tgctccctgc gacggttccg
     3241 accgacgacc cggacgcgat ggtcggcagc acgctgctgc ggtgccgctc gaacgggcga
     3301 tccgactcgt cgacgtggcc tgtgcacgcc gcccggacgc gccggcctca atcctgcgag
     3361 ccgcggacga cagcgggccg acaactacct gctgccgtgc tcgacgaccc cggccacagc
     3421 cgtgctgcac ggtgaacgcg ccagcctcac gtccggccgc acacgacatg aacatcagtc
     3481 atatttgcga ctgtggaaat cgtcccgcgt tcgagatgcc gcgcaaacgg gaagtggctt
     3541 ggggaatgcc ctgcgacaca atggttatct tcgagattcg cgcagtttgt ccttgacagc
     3601 atgagcgagc acgcggcagc atggcaccac ccccaggagg tgagacatca atccctcatc
     3661 ttctccgttc atccggttcc gtgctcaaac gcggaataga gaggagagtg ttagggccat
     3721 gcatgctgat gcactgcgtt cgtgccatat ataacccggt
//
LOCUS       DAOSUG010000300         3736 bp    DNA     linear   ENV 10-JUN-2024
DEFINITION  MAG TPA_asm: Micropruina sp. isolate AMR_MDS_2195
            NODE_10190_length_3736_cov_4.883456, whole genome shotgun sequence.
ACCESSION   DAOSUG010000300 DAOSUG010000000
VERSION     DAOSUG010000300.1
DBLINK      BioProject: PRJNA925477
            BioSample: SAMN32864262
            Sequence Read Archive: SRR4236673
KEYWORDS    WGS; ENV; Metagenome Assembled Genome; MAG; Third Party Data; TPA;
            TPA:assembly.
SOURCE      Micropruina sp. (wastewater metagenome)
  ORGANISM  Micropruina sp.
            Bacteria; Bacillati; Actinomycetota; Actinomycetes;
            Propionibacteriales; Nocardioidaceae; Micropruina.
REFERENCE   1  (bases 1 to 3736)
  AUTHORS   Abdulkadir,N., Saraiva,J.P., Zhang,J., Stolte,S., Gillor,O.,
            Harms,H. and Rocha,U.
  TITLE     Genome-centric analyses of 165 metagenomes show that mobile genetic
            elements are crucial for the transmission of antimicrobial
            resistance genes to pathogens in activated sludge and wastewater
  JOURNAL   Microbiol Spectr 12 (3), e0291823 (2024)
   PUBMED   38289113
REFERENCE   2  (bases 1 to 3736)
  AUTHORS   Abdulkadir,N.
  TITLE     Direct Submission
  JOURNAL   Submitted (24-MAR-2023) Environmental Microbiology, Helmholtz
            Center for Environmental Research -UFZ, Permoserstr 15, Leipzig
            04318, Germany
COMMENT     The annotation was added by the NCBI Prokaryotic Genome Annotation
            Pipeline (PGAP). Information about PGAP can be found here:
            https://www.ncbi.nlm.nih.gov/genome/annotation_prok/
            
            ##Genome-Assembly-Data-START##
            Assembly Method        :: SPAdes v. 3.11.1
            Genome Representation  :: Full
            Expected Final Version :: Yes
            Genome Coverage        :: Not Applicable
            Sequencing Technology  :: Illumina MiSeq
            ##Genome-Assembly-Data-END##
            
            ##Genome-Annotation-Data-START##
            Annotation Provider               :: NCBI
            Annotation Date                   :: 05/04/2023 04:57:10
            Annotation Pipeline               :: NCBI Prokaryotic Genome
                                                 Annotation Pipeline (PGAP)
            Annotation Method                 :: Best-placed reference protein
                                                 set; GeneMarkS-2+
            Annotation Software revision      :: 6.5
            Features Annotated                :: Gene; CDS; rRNA; tRNA; ncRNA
            Genes (total)                     :: 2,776
            CDSs (total)                      :: 2,750
            Genes (coding)                    :: 2,714
            CDSs (with protein)               :: 2,714
            Genes (RNA)                       :: 26
            tRNAs                             :: 24
            ncRNAs                            :: 2
            Pseudo Genes (total)              :: 36
            CDSs (without protein)            :: 36
            Pseudo Genes (ambiguous residues) :: 0 of 36
            Pseudo Genes (frameshifted)       :: 2 of 36
            Pseudo Genes (incomplete)         :: 32 of 36
            Pseudo Genes (internal stop)      :: 2 of 36
            ##Genome-Annotation-Data-END##
FEATURES             Location/Qualifiers
     source          1..3736
                     /organism="Micropruina sp."
                     /mol_type="genomic DNA"
                     /submitter_seqid="NODE_10190_length_3736_cov_4.883456"
                     /isolate="AMR_MDS_2195"
                     /isolation_source="Activated sludge of municipal
                     wastewater treatment plants"
                     /db_xref="taxon:2737536"
                     /environmental_sample
                     /geo_loc_name="Canada"
                     /lat_lon="49.25 N 123.1 W"
                     /collection_date="2015-06-16"
                     /metagenome_source="wastewater metagenome"
                     /note="metagenomic"
     gene            complement(<1..630)
                     /locus_tag="PLF56_09895"
     CDS             complement(<1..630)
                     /locus_tag="PLF56_09895"
                     /inference="COORDINATES: protein motif:HMM:NF012742.2"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_function: GO:0000976 - transcription cis-regulatory
                     region binding [Evidence IEA];
                     GO_function: GO:0003700 - DNA-binding transcription factor
                     activity [Evidence IEA];
                     GO_function: GO:0043565 - sequence-specific DNA binding
                     [Evidence IEA];
                     GO_process: GO:0006351 - DNA-templated transcription
                     [Evidence IEA];
                     GO_process: GO:0006355 - regulation of DNA-templated
                     transcription [Evidence IEA];
                     GO_process: GO:0045892 - negative regulation of
                     DNA-templated transcription [Evidence IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="LacI family DNA-binding transcriptional
                     regulator"
                     /protein_id="HOQ53913.1"
                     /translation="MVRGGAARGRDATRDDVARLAGVSSAVVSYVVNDGPRPVAAATK
                     ARVLDAIDKLGYRPNTAARSLITGRSDLIGLVVPDVENQYFATLAKAVEKAAAARGVR
                     LVLGQAQPDYLPELVESLAGHQVDGIITATPPPADLIARTGRLTFPLVKLSLDMSMDA
                     VPSLSPDFYGGTQSAVRHLAEVHGHRRIALVTGGSPMDVRERAWVDALHG"
     gene            complement(655..1632)
                     /locus_tag="PLF56_09900"
     CDS             complement(655..1632)
                     /locus_tag="PLF56_09900"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_017933033.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="pirin family protein"
                     /protein_id="HOQ53914.1"
                     /translation="MSNTETRAEVHECVTDAAHDPREIQRIEPKSVQLGEDALKVRRT
                     LPSLRRSFVGAWCFADHYGPEHGICMDVPPHPHTGLQTVSWLFEGEIEHRDSGGVHAM
                     VRPGELNLMTSGYGIAHSEVSTPERELLHGVQLWVALPEHDRDLVREFQHHAPDPVEL
                     DGVHARVLTGSLAGSTSPVHTQTPLLGAEVILDPGVTWEVAVEAGFEHGVLVDTGHVD
                     FDGVRLDRSVLGVRDAGLDHLRLTNPTDAPARVMLLGGEPFDEGIVMWWNFIGRSHDD
                     IVRMREEWNDAPDDRFGRVAGYRGRTPRLAAPPLPAELRLKRRFRRGRQ"
     gene            complement(1894..2745)
                     /locus_tag="PLF56_09905"
     CDS             complement(1894..2745)
                     /locus_tag="PLF56_09905"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_014847485.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="MetQ/NlpA family ABC transporter
                     substrate-binding protein"
                     /protein_id="HOQ53915.1"
                     /translation="MGTFRKLLAVAAASALTLALGACSSAAPATTATPAGATTSSAAL
                     TTVTVGASPVPHAKILEFVKQNLAPAAGLDLKIVEFDDYVQPNEALASKELDANYFQH
                     LPYLEEQIKERGYAFSHGTGIHIEPYAAFSKKYKSLAELPQGATIAITNDASNQIRGL
                     RLLEGAGLLTGITDSSNVLNLTKAQNPKGFVFVENQPEVIVQQLDDPKIDLAFVNGNF
                     ILTAGLSTKDALAVEGVKDNPYANLLVWRTDNTNPGVAKLEELLHSQQVKDFITKTWP
                     SGDVLPG"
     gene            complement(2904..3587)
                     /locus_tag="PLF56_09910"
     CDS             complement(2904..3587)
                     /locus_tag="PLF56_09910"
                     /EC_number="7.4.2.11"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_014847486.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_function: GO:0033232 - ABC-type D-methionine
                     transporter activity [Evidence IEA];
                     GO_function: GO:0042626 - ATPase-coupled transmembrane
                     transporter activity [Evidence IEA];
                     GO_process: GO:0048473 - D-methionine transport [Evidence
                     IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="methionine ABC transporter permease"
                     /protein_id="HOQ53916.1"
                     /translation="MIDFWQSLITNPAITTGLWPAFLETLQMVGIAGLATVLFGLPLG
                     VVLFVTQPGGLAANRPVNVVLGGVLVNITRSIPYAILMVTLIPFTRWLVGTSIGPIAA
                     SVSLTIACVPFFARLVEASLREVHPGKVDAAHAMGSTRFQTVYKVLIPEALPALVASI
                     TTTVVALIGYSAMAGLIGGGGLGRLAYNYGYQRFQPEVMVVVLIVLVLLVQLVQVAGD
                     LIVRRVDHR"
BASE COUNT          562 a         1256 c         1336 g          582 t
ORIGIN      
        1 gccgtgcagc gcatcgaccc aggcccgttc gcgcacgtcc atcggtgagc cgccggtgac
       61 cagcgcgata cggcggtgcc cgtgcacctc ggccaggtgc cgcaccgccg actgcgtgcc
      121 gccgtagaag tcgggggaga ggctgggcac cgcgtccatc gacatgtcga ggctgagctt
      181 gaccagggga aacgtcagtc gtccggtgcg ggcgatcagg tcggccggcg gtggggtggc
      241 ggtgatgatg ccgtccacct gatggccggc cagcgactcg accaactcgg gcaggtagtc
      301 gggttgcgcc tgccccaaca ccagccggac gccgcgtgcg gccgccgcct tctcgaccgc
      361 cttggccagg gtcgcgaagt actggttctc gacgtcgggc acgaccaggc cgatcaggtc
      421 ggagcgtccg gtgatcagcg agcgggccgc cgtgttgggc cggtagccca gcttgtcgat
      481 cgcgtccagc acccgggcct tggtggccgc cgcgaccgga cggggcccgt cgttgaccac
      541 gtagctgacc acggcactgc tcactccggc cagccgggcg acgtcgtcgc gggtggcgtc
      601 gcgacccctg gccgcaccac ctcgcaccat cacaccccct cgcctgcggt cactctactg
      661 gcggccgcgg cgaaacctgc gcttgagccg cagctcggcc ggcaacgggg gtgcggccag
      721 gcgtggggtg cggccgcggt aaccggcgac ccggccgaag cggtcgtcgg gggcatcgtt
      781 ccactcctcg cgcatgcgga cgatgtcgtc gtggctacgt ccgatgaagt tccaccacat
      841 cacgatgccc tcgtcgaagg gttcaccccc gagcagcatc acccgggccg gcgcgtcggt
      901 cgggttggtc agccgcagat ggtcgaggcc cgcgtcgcgc acgcccaaca cgctgcggtc
      961 gaggcgcacg ccgtcgaagt cgacgtgacc ggtgtcgacc agcacaccat gctcgaagcc
     1021 ggcctcgacc gcgacctccc acgtgacgcc cgggtcgaga atcacctcgg cgcccagcaa
     1081 cggggtctgg gtgtgcacgg gcgaggtgct gccggccagc gagccggtca ggacccgggc
     1141 gtgaaccccg tccagttcga ccgggtcggg ggcatggtgt tgaaactcgc gcaccaggtc
     1201 gcggtcgtgc tcgggcaacg ccacccacag ctggacgccg tgcagcagct cgcgctcagg
     1261 ggtgctgacc tcggagtgcg cgatgccgta gccggaggtc atcaggttca gctcgcccgg
     1321 ccgcaccatg gcgtgcaccc cgcccgagtc ccggtgctcg atctcgccct cgaacagcca
     1381 gctgacggtc tgcagcccgg tgtgcgggtg cgggggaacg tccatgcaga tgccgtgttc
     1441 gggcccgtag tgatcggcga agcaccaggc gcccacgaac gaacggcgta gtgaaggcag
     1501 ggtccgtcgc accttgaggg cgtcctcgcc cagctggacg ctcttgggtt cgatccgctg
     1561 gatctcgcgc gggtcgtggg cggcatcggt gacgcactcg tgcacctcgg cccgggtctc
     1621 ggtgttgctc atgaggcacc tcctggtcgc gtgccggggc ggccagccta tgcgcggatc
     1681 cggtttgcgt cgcggcgtcc ggcgcggatc cgggctacgt cgggctgtgc cggagcgtcc
     1741 aggatcggcc cgacctgctg cgaacgacga gagacccggg acgacctgcg tcccgggtcc
     1801 cgcattgcgc tcggcccaag tggggagcgg cccactcagg agttcccccc tgatcgcggc
     1861 tcacccctga tcagggtgca gcccgcacgc tactcagccg ggcagcacgt cgcccgaggg
     1921 ccaggtcttg gtgatgaagt ccttgacctg ctgggaatgc agcagctcct cgagcttggc
     1981 cacgcccggg ttggtgttgt ccgtgcgcca gaccagcagg ttggcgtacg ggttgtcctt
     2041 gacgccctcg accgccagcg cgtccttggt cgacaggccc gcggtcagaa tgaagttgcc
     2101 gttgacgaac gcgagatcga tcttcgggtc gtccaactgc tgcacgatca cctcgggctg
     2161 gttctcgacg aacacgaacc ccttcgggtt ctgcgccttg gtcaggttca gcacgttgga
     2221 gctgtcagtg atgccggtca gcagcccggc gccctcgagc agccgcagcc cgcggatctg
     2281 attggacgcg tcgttggtga tcgcgatggt cgcaccctgg ggcagctcgg ccagcgactt
     2341 gtacttcttc gagaacgcgg cgtacggctc gatgtgaatg ccggtgccgt gcgagaacgc
     2401 gtagccccgc tccttgatct gttcctcgag gtagggcagg tgctggaagt agttggcgtc
     2461 cagttccttg ctggccaggg cctcgttcgg ctgcacgtag tcgtcgaact cgacgatctt
     2521 gaggtcgaga ccggcggccg gcgccaggtt ctgcttgacg aactcaagga tcttggcgtg
     2581 cggcaccggg ctggcgccga ccgtcaccgt ggtgagtgcg gccgaggacg tggtcgcgcc
     2641 ggccggcgtc gctgtcgtgg ccggggctgc gctgctgcag gcgcccagcg cgagggtcag
     2701 ggcggacgcc gccgcaacgg cgagcagctt tctgaaggtt cccatgattc tccttgtgga
     2761 tcgccggtcg tgggtgctga ctggctggtg tgaccgggcc cggatcgggc gcggaagagt
     2821 ggggtcggac ggaatgaagt gcgcgaaccc gagcgatccg tcctgctgtg aactcggacg
     2881 cggtggacgc gaggtgggcg ggttcatcgg tggtccaccc ggcgcacgat gaggtcgccg
     2941 gccacctgga ccagctgcac cagcagcacc agcacgatca gcaccacgac catcacctcg
     3001 ggctgaaaac gctggtagcc gtagttgtac gcgagcctgc ccaggccgcc gccgccgatc
     3061 aggccggcca tcgccgagta cccgatcagg gccacgacgg tggtggtgat gctggccacc
     3121 agcgccggca gggcctcggg aatgagcact ttgtacacgg tctggaagcg ggtcgagccc
     3181 atggcgtggg cggcgtccac cttgcccgga tgcacctcgc gcagcgaggc ctcgaccagc
     3241 ctggcgaaga acggcacaca ggcgatggtc agcgacactg acgcggcgat cggaccgatc
     3301 gaggtgccga ccagccatcg ggtgaacgga atcagcgtca ccatcaggat cgcgtaggga
     3361 atggagcggg tgatgttcac cagcaccccg cccagcacca cgttgaccgg acggttggcg
     3421 gccaggccgc ccggttgggt gacgaacagc accacaccga gcggcaaccc gaacagcacc
     3481 gtcgcaaggc cggcgatgcc gaccatctgc agggtttcga gaaaggcggg ccacaacccg
     3541 gtggtgatcg ccgggttggt gatcaggctc tgccagaagt cgatcatcgc gccacctcgt
     3601 cgccggttcg ggccgccgag gcgccgagcc tgcgcaggcc ggccaccacc tcgtccgggc
     3661 ggaggggcgg tgggaccagt gcccgatgcc gcgcgaaggt gacgccgccg agggtttcga
     3721 cggtgccggc gaccac
//
LOCUS       DAOSUG010000301         3736 bp    DNA     linear   ENV 10-JUN-2024
DEFINITION  MAG TPA_asm: Micropruina sp. isolate AMR_MDS_2195
            NODE_10191_length_3736_cov_4.029612, whole genome shotgun sequence.
ACCESSION   DAOSUG010000301 DAOSUG010000000
VERSION     DAOSUG010000301.1
DBLINK      BioProject: PRJNA925477
            BioSample: SAMN32864262
            Sequence Read Archive: SRR4236673
KEYWORDS    WGS; ENV; Metagenome Assembled Genome; MAG; Third Party Data; TPA;
            TPA:assembly.
SOURCE      Micropruina sp. (wastewater metagenome)
  ORGANISM  Micropruina sp.
            Bacteria; Bacillati; Actinomycetota; Actinomycetes;
            Propionibacteriales; Nocardioidaceae; Micropruina.
REFERENCE   1  (bases 1 to 3736)
  AUTHORS   Abdulkadir,N., Saraiva,J.P., Zhang,J., Stolte,S., Gillor,O.,
            Harms,H. and Rocha,U.
  TITLE     Genome-centric analyses of 165 metagenomes show that mobile genetic
            elements are crucial for the transmission of antimicrobial
            resistance genes to pathogens in activated sludge and wastewater
  JOURNAL   Microbiol Spectr 12 (3), e0291823 (2024)
   PUBMED   38289113
REFERENCE   2  (bases 1 to 3736)
  AUTHORS   Abdulkadir,N.
  TITLE     Direct Submission
  JOURNAL   Submitted (24-MAR-2023) Environmental Microbiology, Helmholtz
            Center for Environmental Research -UFZ, Permoserstr 15, Leipzig
            04318, Germany
COMMENT     The annotation was added by the NCBI Prokaryotic Genome Annotation
            Pipeline (PGAP). Information about PGAP can be found here:
            https://www.ncbi.nlm.nih.gov/genome/annotation_prok/
            
            ##Genome-Assembly-Data-START##
            Assembly Method        :: SPAdes v. 3.11.1
            Genome Representation  :: Full
            Expected Final Version :: Yes
            Genome Coverage        :: Not Applicable
            Sequencing Technology  :: Illumina MiSeq
            ##Genome-Assembly-Data-END##
            
            ##Genome-Annotation-Data-START##
            Annotation Provider               :: NCBI
            Annotation Date                   :: 05/04/2023 04:57:10
            Annotation Pipeline               :: NCBI Prokaryotic Genome
                                                 Annotation Pipeline (PGAP)
            Annotation Method                 :: Best-placed reference protein
                                                 set; GeneMarkS-2+
            Annotation Software revision      :: 6.5
            Features Annotated                :: Gene; CDS; rRNA; tRNA; ncRNA
            Genes (total)                     :: 2,776
            CDSs (total)                      :: 2,750
            Genes (coding)                    :: 2,714
            CDSs (with protein)               :: 2,714
            Genes (RNA)                       :: 26
            tRNAs                             :: 24
            ncRNAs                            :: 2
            Pseudo Genes (total)              :: 36
            CDSs (without protein)            :: 36
            Pseudo Genes (ambiguous residues) :: 0 of 36
            Pseudo Genes (frameshifted)       :: 2 of 36
            Pseudo Genes (incomplete)         :: 32 of 36
            Pseudo Genes (internal stop)      :: 2 of 36
            ##Genome-Annotation-Data-END##
FEATURES             Location/Qualifiers
     source          1..3736
                     /organism="Micropruina sp."
                     /mol_type="genomic DNA"
                     /submitter_seqid="NODE_10191_length_3736_cov_4.029612"
                     /isolate="AMR_MDS_2195"
                     /isolation_source="Activated sludge of municipal
                     wastewater treatment plants"
                     /db_xref="taxon:2737536"
                     /environmental_sample
                     /geo_loc_name="Canada"
                     /lat_lon="49.25 N 123.1 W"
                     /collection_date="2015-06-16"
                     /metagenome_source="wastewater metagenome"
                     /note="metagenomic"
     gene            <1..617
                     /locus_tag="PLF56_09915"
     CDS             <1..617
                     /locus_tag="PLF56_09915"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_008360717.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_function: GO:0003824 - catalytic activity [Evidence
                     IEA];
                     GO_function: GO:0030976 - thiamine pyrophosphate binding
                     [Evidence IEA]"
                     /codon_start=3
                     /transl_table=11
                     /product="thiamine pyrophosphate-dependent enzyme"
                     /protein_id="HOQ53917.1"
                     /translation="DDDAIFIPDVGTPVIWAARYLRMNGKRSLLGSFVHGSMANALPQ
                     ATGAAVAQPGRQVVALSGDGGVTMLLGELLTLTQNHLPVTIVVFNNGSLNFVELEMKA
                     TGFVNYATDLENPDLAAVASAVGLKGIRVTRSDQLQDALREALAHDGPALVDVVTERQ
                     ELTIPPSVSVEQATGFALYAMRTLLSGRGDELLDLTRTNLRQVF"
     gene            717..1028
                     /locus_tag="PLF56_09920"
     CDS             717..1028
                     /locus_tag="PLF56_09920"
                     /inference="COORDINATES: protein motif:HMM:NF024240.2"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="FAD-dependent oxidoreductase"
                     /protein_id="HOQ53918.1"
                     /translation="MAYDHDLLVVGGGPGGHRAAIAAAELGRRVAIVDRKQLIGGVCI
                     NTGTIAFTTRGRAVPDRYAGRLAAVPFMGEEPGDITALQPIGISGIPEISTYASSRRT
                     P"
     gene            complement(1894..2931)
                     /locus_tag="PLF56_09925"
     CDS             complement(1894..2931)
                     /locus_tag="PLF56_09925"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_013865041.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="glutathione S-transferase C-terminal
                     domain-containing protein"
                     /protein_id="HOQ53919.1"
                     /translation="MTDGQAEHGKGGVYSSAGKEFVRDTRYINDRIVADPQAPDEWPV
                     EPGRYRLIAARACPWANRAIIVRRLLGLEDAFSLGLPGPTHDSDSWTFDLDPGGVDPV
                     LRIHKLKDAYLNRFPDYPRGITVPAMVEVATGEVVTNDFAQITEDFATQWTRYQRDGA
                     PDLWPAAHSDEQHEVMRRIYTEVNNGVYRCGFAGSQESYDAAFERLWTALDWLESRLS
                     TQRYLIGDTITEADVRLFTTLVRFDPVYHGHFKCNRSKLAELPALWGYARDLYQTPGF
                     GDTVDFVQIKQHYYIVHRDLNPLGIVPDGPDLSNWLTEHGRERLGGRPFGDGTPPGPV
                     RRGEEVPNPLA"
     gene            complement(2971..>3736)
                     /locus_tag="PLF56_09930"
     CDS             complement(2971..>3736)
                     /locus_tag="PLF56_09930"
                     /inference="COORDINATES: protein motif:HMM:NF024732.2"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=2
                     /transl_table=11
                     /product="acetyl-CoA hydrolase/transferase C-terminal
                     domain-containing protein"
                     /protein_id="HOQ53920.1"
                     /translation="DGTAPMIELAPSVIGPTQVAIAEHVAALIPDGSTLQIGIGGIPD
                     AVVKQLMTKNDLGLHTEMLGDGTLSLIEAGVITNTRKNLHPGKLVATFALGSARLYEF
                     MDHHPHLEMHPVDYTNAPASASRNDNLHSINGTLAVDFFGQCASESLGRRPFSGPGGQ
                     VDFVRAANASNGGTSIIVLPATAKGDTISRIVAGHGPGTPITTGKNDVDHVVTEFGVA
                     RLRGRSVRDRTRALIEIAHPAFRDELTQQAKDLRLW"
BASE COUNT          587 a         1243 c         1286 g          620 t
ORIGIN      
        1 ccgacgacga tgcgatcttc attcccgacg tgggcacccc ggtgatctgg gccgcgcgct
       61 acctgcggat gaacggcaag cggagcctgc tgggatcgtt cgtgcacgga tccatggcca
      121 acgcactgcc gcaggcgacc ggtgcggcgg tcgcacaacc cggacgccag gtggtcgccc
      181 tgtcgggcga cggcggcgtg acgatgctgc tcggagaact gctgaccctg acgcagaatc
      241 acctgccggt caccatcgtg gtgttcaaca acggctcgct caacttcgtg gagctggaga
      301 tgaaggccac gggtttcgtc aactacgcca ctgacctgga gaaccccgac ctggcggcgg
      361 tggcctccgc cgtggggttg aagggcatcc gggtcacccg ctccgatcag ttgcaggacg
      421 cgctgcgcga agcactggcc cacgatggac cggccctggt cgacgtggta accgagcggc
      481 aggaactgac cattccgccg tccgtcagcg tggaacaggc cacgggcttc gcgctctacg
      541 cgatgcgcac cctgctgtcc ggacgcggtg acgaactgct cgacctgacc cgcacgaacc
      601 ttcgccaagt gttctgagcg gcctgtcgcg gcgtgcgggc ggttcctgcg ccgagacgca
      661 ttgccgtcga gtgttgtcgg ggcgcataag cggcggccgc acggacggag gccgccatgg
      721 cgtacgacca cgacctgctc gtggtgggcg ggggcccggg cggtcaccgc gccgccatcg
      781 ccgcggcgga actcggccgc cgggtggcga tcgtcgaccg caagcagctg atcggcgggg
      841 tgtgcatcaa caccggcact attgcgttca cgacacgagg ccgtgctgta cctgaccggt
      901 atgcgggccg gctggccgcg gtgcccttca tgggagaaga gccgggcgac atcaccgcgc
      961 tgcagccgat cggcatctcc ggcattcccg agatcagcac ctatgcgtca tcccggcgaa
     1021 cgccgtgatc tcagccccga agacgaggtc ttgtagcctc tcgagcttgt cggaaccgat
     1081 ccttggtaga tttcgccagc atcggtcacc atcaatcggc cgccctcgaa agatggagtg
     1141 aatccgcctc ccgaagtcgg tacaccagtt cactccttgt tcaggagcat gcgcgccggg
     1201 tcgggagcac cgcccgcccc gctccactgt cgctcgtgga cgaagccacc atccgccgaa
     1261 cgccgggatc tcaaccccgg agacgaggtc ttgtagcctc tggagcctgt cgggagcgat
     1321 tgttgctaga tttcgccgcc gtcggtcacc atcaatcggc cgccctcgaa agatggagtg
     1381 aatctgccta ccgaagtcgg tacaccagtt cactccttgt tcgggagcct gcgcgccggg
     1441 tcggtagcac cttccgcccc gctccactgt cgttcgtgga caatgccacc atccaccatt
     1501 ggtcatccag gcgttcgccg ggatcccaca cactgctggt cacggttgtc gggacctgcc
     1561 gtgggccgag atcccggcgt tcgccgggct gacggggacg gccatgagtg gagatccggc
     1621 gttcgccggg atgacgggac gggcgaacat gacgggacgg ccggaccccc cgcttgctcc
     1681 gggctgacgg ggacggccat gagtggagat ccggcgttcg cccgggatgt cgggacgggc
     1741 ggacatgacg ggacggccgg accccccgct tgctccggga tgacggggac ggccatgagt
     1801 ggagatcccg gctgtgagtt cgccggatgt cggggcgggc ggtcatctcg ggacggccag
     1861 acccccggct tgctccagga tgactcccga ggctcaagcc agcgggttcg gcacctcttc
     1921 cccccgacgg acggggcccg ggggagtccc gtcaccgaac gggcggccac cgagcctctc
     1981 gcggccgtgc tccgtcagcc agttcgagag gtcaggcccg tcgggcacga tgcccagcgg
     2041 gttcaggtca cggtgaacga tgtagtagtg ctgcttgatc tgcacgaagt ccaccgtgtc
     2101 gccgaagcca ggggtctggt acaggtcgcg ggcgtagccc cacagtgccg gtagctccgc
     2161 gagcttcgac cggttgcact tgaagtgccc gtggtacacc gggtcgaagc gcaccagcgt
     2221 ggtgaacagc cgcacgtccg cctcggtgat cgtgtcgccg atcagatacc gctgcgtgct
     2281 caagcgcgac tcgagccagt ccagggccgt ccacaaccgc tcgaaggccg cgtcatagct
     2341 ctcctggcta ccggcgaagc cgcatcggta cacgccgttg ttcacctcgg tgtagatgcg
     2401 acgcatcacc tcgtgctgct cgtcggagtg cgccgccggc cacagatcgg gggccccgtc
     2461 gcgctgatag cgcgtccact gggtggcgaa gtcctcggtg atctgcgcga agtcgttggt
     2521 gaccacctcg ccggtggcca cctcgaccat cgccggaacg gtgatgccgc gcgggtagtc
     2581 gggaaagcga ttcaggtagg cgtccttgag cttgtggatg cgcagtaccg gatccacacc
     2641 gcccgggtcg aggtcgaacg tccacgagtc ggaatcgtgg gtggggccgg gcaggccgag
     2701 cgaaaaggcg tcctccagcc cgagcagccg ccgcacgatg atcgcccggt tcgcccacgg
     2761 gcaggcccgc gcggcgatca gccgatagcg cccgggctcg acgggccact cgtccggcgc
     2821 ctgcggatcg gccacgatcc ggtcgttgat gtagcgggtg tcacgcacga actccttgcc
     2881 cgcgctggag tacaccccac ccttgccgtg ttctgcctgg ccatcggtca tgcccccacc
     2941 gtaggcggcc cgatcgggac agggccagac tcaccagagg cgcagatcct tcgcctgctg
     3001 ggtcagctca tcgcggaagg ccgggtgtgc gatctcgatc agcgcacgcg tacggtcgcg
     3061 gacggagcgt ccacgcagcc gggccacgcc gaactccgtc accacgtggt cgacgtcgtt
     3121 cttgccggtg gtgatcgggg tgccggggcc gtgaccggcc acgatgcggc tgatcgtgtc
     3181 gcccttggcg gtggcgggca gcacgatgat cgacgtgccg ccgtttgacg cgttcgcggc
     3241 acggacgaag tccacctggc cacccggccc cgagaacgga cggcgtccca atgattcgga
     3301 cgcgcactgg ccgaagaagt ccaccgccag ggtgccgttg atggagtgca ggttgtcgtt
     3361 gcggctggcc gaggcggggg cgttggtgta gtcgaccggg tgcatctcga ggtgcgggtg
     3421 gtggtccatg aactcgtaca accgcgccga gccgagcgcg aaggtggcga ccaacttgcc
     3481 cgggtgcagg ttcttgcggg tgttggtgat cacccccgcc tcgatcagcg acagcgtgcc
     3541 gtcgcccagc atctcggtgt gcagacccag gtcgttcttg gtcatcagtt gcttgaccac
     3601 cgcgtccgga atgccgccga tgccgatctg cagggtgctg ccgtccggaa tcagagcggc
     3661 gacgtgctcg gcgatcgcga cctgggtggg gccgatcacg gacggcgcca actcgatcat
     3721 gggtgccgtg ccgtcg
//
LOCUS       DAOSUG010000302         3733 bp    DNA     linear   ENV 10-JUN-2024
DEFINITION  MAG TPA_asm: Micropruina sp. isolate AMR_MDS_2195
            NODE_10204_length_3733_cov_3.936922, whole genome shotgun sequence.
ACCESSION   DAOSUG010000302 DAOSUG010000000
VERSION     DAOSUG010000302.1
DBLINK      BioProject: PRJNA925477
            BioSample: SAMN32864262
            Sequence Read Archive: SRR4236673
KEYWORDS    WGS; ENV; Metagenome Assembled Genome; MAG; Third Party Data; TPA;
            TPA:assembly.
SOURCE      Micropruina sp. (wastewater metagenome)
  ORGANISM  Micropruina sp.
            Bacteria; Bacillati; Actinomycetota; Actinomycetes;
            Propionibacteriales; Nocardioidaceae; Micropruina.
REFERENCE   1  (bases 1 to 3733)
  AUTHORS   Abdulkadir,N., Saraiva,J.P., Zhang,J., Stolte,S., Gillor,O.,
            Harms,H. and Rocha,U.
  TITLE     Genome-centric analyses of 165 metagenomes show that mobile genetic
            elements are crucial for the transmission of antimicrobial
            resistance genes to pathogens in activated sludge and wastewater
  JOURNAL   Microbiol Spectr 12 (3), e0291823 (2024)
   PUBMED   38289113
REFERENCE   2  (bases 1 to 3733)
  AUTHORS   Abdulkadir,N.
  TITLE     Direct Submission
  JOURNAL   Submitted (24-MAR-2023) Environmental Microbiology, Helmholtz
            Center for Environmental Research -UFZ, Permoserstr 15, Leipzig
            04318, Germany
COMMENT     The annotation was added by the NCBI Prokaryotic Genome Annotation
            Pipeline (PGAP). Information about PGAP can be found here:
            https://www.ncbi.nlm.nih.gov/genome/annotation_prok/
            
            ##Genome-Assembly-Data-START##
            Assembly Method        :: SPAdes v. 3.11.1
            Genome Representation  :: Full
            Expected Final Version :: Yes
            Genome Coverage        :: Not Applicable
            Sequencing Technology  :: Illumina MiSeq
            ##Genome-Assembly-Data-END##
            
            ##Genome-Annotation-Data-START##
            Annotation Provider               :: NCBI
            Annotation Date                   :: 05/04/2023 04:57:10
            Annotation Pipeline               :: NCBI Prokaryotic Genome
                                                 Annotation Pipeline (PGAP)
            Annotation Method                 :: Best-placed reference protein
                                                 set; GeneMarkS-2+
            Annotation Software revision      :: 6.5
            Features Annotated                :: Gene; CDS; rRNA; tRNA; ncRNA
            Genes (total)                     :: 2,776
            CDSs (total)                      :: 2,750
            Genes (coding)                    :: 2,714
            CDSs (with protein)               :: 2,714
            Genes (RNA)                       :: 26
            tRNAs                             :: 24
            ncRNAs                            :: 2
            Pseudo Genes (total)              :: 36
            CDSs (without protein)            :: 36
            Pseudo Genes (ambiguous residues) :: 0 of 36
            Pseudo Genes (frameshifted)       :: 2 of 36
            Pseudo Genes (incomplete)         :: 32 of 36
            Pseudo Genes (internal stop)      :: 2 of 36
            ##Genome-Annotation-Data-END##
FEATURES             Location/Qualifiers
     source          1..3733
                     /organism="Micropruina sp."
                     /mol_type="genomic DNA"
                     /submitter_seqid="NODE_10204_length_3733_cov_3.936922"
                     /isolate="AMR_MDS_2195"
                     /isolation_source="Activated sludge of municipal
                     wastewater treatment plants"
                     /db_xref="taxon:2737536"
                     /environmental_sample
                     /geo_loc_name="Canada"
                     /lat_lon="49.25 N 123.1 W"
                     /collection_date="2015-06-16"
                     /metagenome_source="wastewater metagenome"
                     /note="metagenomic"
     gene            <1..424
                     /locus_tag="PLF56_09935"
     CDS             <1..424
                     /locus_tag="PLF56_09935"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_016666973.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=2
                     /transl_table=11
                     /product="oxidoreductase"
                     /protein_id="HOQ53921.1"
                     /translation="LDYLKLGKGPYYALYRPYHLASIEANLSIGEAIIDGRSTFHPIG
                     WTSEVTAVAKSDLTAGTRLEGIGGHHVHGFTVPAADASAANAVPIGLIAGCTLVRDVA
                     AGTTVSYADVELDESRPIVAMRRLQDALLAKGSLPVAS"
     gene            511..1479
                     /locus_tag="PLF56_09940"
     CDS             511..1479
                     /locus_tag="PLF56_09940"
                     /inference="COORDINATES: protein motif:HMM:NF016114.2"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_function: GO:0030246 - carbohydrate binding [Evidence
                     IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="sugar-binding domain-containing protein"
                     /protein_id="HOQ53922.1"
                     /translation="MATTRSIDTLIRAARLYYEEGMSQGEVARTLGISGATVSRVLAA
                     ARDQGIVEVRIHDPRSPVQRVHEYEQELVQMFGLSEVRVGTSAGEASSFRLVGRLAAD
                     LFGERLSDMRQVGLSWGTTLEAFVAEVPQRVVPSLAVYPMAGGNPGLDKASAGGTLVQ
                     ALARRCGVEPGRLIAPAVVESPRTKEAIMSESGIKSALALAAQVDHAFVGIGTFGVRS
                     SVTIVEDMLLTEAELGTFLAQKPAGDISGRFFDDNGIELGPPTSERVIGLTLDELRRV
                     PCVVGMAAGHEKARGVLAGLRTGVLDVLVVDLPLAQAVLQRGGHPG"
     gene            1929..2726
                     /gene="ispD"
                     /locus_tag="PLF56_09945"
     CDS             1929..2726
                     /gene="ispD"
                     /locus_tag="PLF56_09945"
                     /EC_number="2.7.7.60"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_002523049.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_function: GO:0050518 - 2-C-methyl-D-erythritol
                     4-phosphate cytidylyltransferase activity [Evidence IEA];
                     GO_process: GO:0019288 - isopentenyl diphosphate
                     biosynthetic process, methylerythritol 4-phosphate pathway
                     [Evidence IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="2-C-methyl-D-erythritol 4-phosphate
                     cytidylyltransferase"
                     /protein_id="HOQ53923.1"
                     /translation="MPEPVPAVIVAAGRGVRFGGPIPKPVATLAGKAVVAMSVEAMAA
                     GGCTHAVVVIPRNGEHHFQPALAASPIPVTLVEGDETRQSSVRRGIKAILQQPELRGA
                     RVVLVHDAVRPLVPATVVERVIRTVSEGAVAVAPAIPVADSIRAEDEHGRLTIVDRNR
                     LRAIQTPQGFDLQVLNASHDYCALQGIECTDDLSVCEAAGHSVSVVKGAMTALKITSA
                     TDLDLAHAFLKLRKGFGRHSFRRVLRHRPFAWVVKPAEAAPVIRSEQ"
     gene            2723..3199
                     /gene="ispF"
                     /locus_tag="PLF56_09950"
     CDS             2723..3199
                     /gene="ispF"
                     /locus_tag="PLF56_09950"
                     /EC_number="4.6.1.12"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_020578975.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_function: GO:0008685 - 2-C-methyl-D-erythritol
                     2,4-cyclodiphosphate synthase activity [Evidence IEA];
                     GO_process: GO:0019288 - isopentenyl diphosphate
                     biosynthetic process, methylerythritol 4-phosphate pathway
                     [Evidence IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="2-C-methyl-D-erythritol 2,4-cyclodiphosphate
                     synthase"
                     /protein_id="HOQ53924.1"
                     /translation="MNLPRIGIGSDVHALAEGVPMWVAGLHFPDEMRGLAGHSDGDVA
                     CHAICDALLSAAGLGDLGGTVGTDRPEWAGASGVRLLTEIAGLVRGEGFEIGNVAVQV
                     IGNRPRLLPRRAESEALLSRTLGCPVSVSGKTTDGLGMTGRGEGVAAIATALIVRV"
     gene            3196..>3733
                     /locus_tag="PLF56_09955"
     CDS             3196..>3733
                     /locus_tag="PLF56_09955"
                     /inference="COORDINATES: ab initio
                     prediction:GeneMarkS-2+"
                     /note="Derived by automated computational analysis using
                     gene prediction method: GeneMarkS-2+."
                     /codon_start=1
                     /transl_table=11
                     /product="hypothetical protein"
                     /protein_id="HOQ53925.1"
                     /translation="MTDAVALCAHGEASWHALAYRSLGAVWREEAAVARSFGPVPHPY
                     LLGAVTLRPDGEVADDVPGIVCDSYARLRLPGRESEAAGRWMIRAPSGPPSRTAVPGL
                     RIRAAVNDDDVAWFEYLAFLSAGGELPSRSGELHPPGSQRLPGLTLLIAEIDGEGVGT
                     ALSVVTPRVNNIGAVAVMP"
BASE COUNT          525 a         1245 c         1357 g          606 t
ORIGIN      
        1 actcgactac ctcaagttgg gcaagggtcc ctactacgcc ctgtaccggc cgtaccacct
       61 ggcctcgatc gaggcgaacc tgtcgatcgg tgaggcgatc atcgacggac gctccacgtt
      121 ccacccgatc ggctggacct cagaggtcac cgccgtggcc aagtcggatc tgacggcggg
      181 caccaggctg gagggcatcg gcggccacca cgtgcacggc ttcaccgtgc cggcggccga
      241 tgcgagcgcc gcgaacgcgg tgccgatcgg cctgatcgcc ggctgcacgc tggtgcgcga
      301 cgtggccgcg ggtacgaccg tgagctacgc cgacgtcgag ctggacgagt cccgtccgat
      361 cgtcgccatg cggcgcctgc aggacgccct gctcgccaag ggatcgctcc ccgtcgcaag
      421 ctgatccgac ccatcccgcc cgggcacctg gagggtgccg gacggtgggt cgaccatccg
      481 ctacctgcgc atcgtccggg aagggacggc atggccacca ctagatcgat cgacactctg
      541 atcagggccg ctcgcctcta ctacgaagag ggcatgtcgc agggcgaggt ggcgcgcacc
      601 ctcggcattt cgggcgccac cgtgtcacgg gtgctcgccg cggcccgcga ccagggcatc
      661 gtcgaggtgc gcatccatga cccacgctca ccggttcaac gggtgcatga gtacgagcag
      721 gaactcgtgc agatgttcgg gctgagcgag gtgcgcgtcg gtacgtcggc gggtgaagcc
      781 agttcctttc ggctggtcgg acggttggcc gcggacctgt tcggcgaacg cctgagcgac
      841 atgaggcagg tgggtctgag ctggggcacc acgctcgagg cgttcgtggc cgaggtgccg
      901 cagcgggtgg tgccctcgct cgccgtgtac ccgatggccg gtggcaaccc gggcctcgac
      961 aaggcttcgg ccggcggcac cctggtgcag gcgctggctc gccgctgcgg tgtcgaaccg
     1021 ggccggctga tcgcgccggc ggtggtcgaa tcaccgcgca ccaaagaggc catcatgtcc
     1081 gagtcaggca tcaaaagcgc cctcgcgctg gccgcgcagg tcgatcatgc cttcgtcggc
     1141 atcggtacct tcggggtgcg ttcgtcggtc acgatcgtcg aggacatgct gctcaccgag
     1201 gccgaactgg gcaccttctt ggcccagaag cctgccggtg acatcagcgg acgcttcttc
     1261 gacgacaacg gcatcgaact ggggccgccg acctcagagc gggtgatcgg gctcaccttg
     1321 gacgagttgc gccgggtgcc ctgcgtggtg ggcatggccg cgggccacga gaaggcccgc
     1381 ggcgtgctcg ccggactgcg caccggggta ctggacgtgc tggtggtcga tctgccgctc
     1441 gcccaggcgg tgttgcaacg cggcggccac ccgggctgag gtatcccccc ggtcgcgcgt
     1501 gtcgaggtcg gtgcgacgcg tgcttcggtc cccgacccga ttcagccgcc cgggcgatct
     1561 cgagatcgcc gctcgatggc gggatgcctg cctggtttcg tgttgcaacc cgaaactgga
     1621 cggcgttccc gtcatcaggc agcgatctcg agatcgccac aggcctgacg atgacctcga
     1681 caccgcgacc gacctcattg tcggttcctg atcctgcgca cggcgcgcgc atctggggcg
     1741 agagaccgcg gccggtccag cactcgccgg gcgagctcgt ccagactcga atctcgccgc
     1801 tgcgtcaact accgctggtc gagcgggttc agcccgccgg cgccgaccaa gggtcgctcg
     1861 ccaccgccgt cgagcgcagg ccaccattta tccgatcgga ggaggtctgc agtcctatac
     1921 tgccggttgt gcctgagccc gtccccgccg tcatcgttgc cgccggacgt ggcgtccgct
     1981 tcggtggacc gatcccgaag ccggtggcga ccctggccgg caaggcagtg gtggcgatgt
     2041 cggtcgaggc gatggccgcc gggggctgca cgcacgcggt cgtggtcata ccgcgcaacg
     2101 gtgagcatca ctttcagccc gcgttggccg cgtcgccgat cccggtgacc ctggtcgagg
     2161 gggacgagac gcggcagtcg tccgtacgtc ggggcatcaa ggcgatcctg cagcagccgg
     2221 agctccgcgg ggcccgggtg gtgctggtgc acgacgcggt gcgtccgctg gtgcccgcaa
     2281 cggtggtcga gcgggtgatc cgcactgtca gtgagggcgc cgttgcggtg gcgccggcga
     2341 tcccggtcgc ggactcgatc cgcgccgaag acgaacacgg ccggctgacc atcgtcgatc
     2401 gcaaccggtt gcgggccatc cagactccgc agggcttcga cctgcaggtg ctgaacgcca
     2461 gtcacgacta ttgcgcactt cagggcatcg aatgcaccga cgacctgtcc gtgtgcgagg
     2521 ccgcggggca ctcggtgtcg gtcgtcaagg gtgcgatgac ggccctgaag atcacctctg
     2581 ccaccgatct cgatttggcc cacgccttct tgaagctgcg caaggggttc ggccgacact
     2641 cctttcgccg ggttcttcgg catcgtcctt ttgcctgggt ggtgaagccc gccgaggcgg
     2701 cgccggtgat ccggagcgag cagtgaacct gccgcgcatc ggcatcggca gcgatgtgca
     2761 cgcgctcgcc gaaggggtgc cgatgtgggt ggccggactg cactttccgg acgagatgcg
     2821 cgggctggcc ggtcactcgg acggcgatgt ggcctgccat gcgatctgcg acgcgctgtt
     2881 gtcggcggcc ggtctgggcg atctgggcgg cacggtcggc accgatcgcc ccgaatgggc
     2941 cggggcgtcg ggcgtgcggc tgctgaccga gatcgccggg ttggtgcgtg gggagggctt
     3001 cgaaatcggc aacgtcgccg tccaggtgat cggcaaccgg ccccggctac tgccgcggcg
     3061 agccgaatcc gaggcgttgc tcagccggac gctcggctgc ccggtctcgg tgtcgggcaa
     3121 gaccacggac gggttgggca tgaccggccg cggcgagggt gttgcggcga tcgcgaccgc
     3181 cctgatcgtc agggtatgac cgacgccgtc gcgctctgcg cgcacggcga ggcgtcctgg
     3241 catgcgctgg cgtaccggtc gctgggtgcg gtgtggcgcg aggaggcggc ggtggcccgc
     3301 tcgttcgggc cggtgccgca tccctatctg ctcggagcgg tcacccttcg ccctgacggc
     3361 gaggtcgccg acgacgtgcc cggcatcgtg tgcgacagct acgcccggct gaggctgccc
     3421 ggacgtgagt ccgaggccgc cgggcgctgg atgatccgcg caccctccgg tccacccagc
     3481 cgaacggcag taccggggct gcggattcgt gcggcggtga atgacgacga cgtggcctgg
     3541 ttcgagtacc tggcctttct gtcggccggg ggtgagctgc cgagccgatc gggcgagctg
     3601 cacccgcccg gaagccagcg gctaccggga ttgacgttgc tgatcgccga gatcgacggc
     3661 gaaggggtcg gcaccgcgct ctcggtggtg acgccccggg taaacaacat cggcgcggtg
     3721 gcggtgatgc ccg
//
LOCUS       DAOSUG010000303         3727 bp    DNA     linear   ENV 10-JUN-2024
DEFINITION  MAG TPA_asm: Micropruina sp. isolate AMR_MDS_2195
            NODE_10215_length_3727_cov_4.892429, whole genome shotgun sequence.
ACCESSION   DAOSUG010000303 DAOSUG010000000
VERSION     DAOSUG010000303.1
DBLINK      BioProject: PRJNA925477
            BioSample: SAMN32864262
            Sequence Read Archive: SRR4236673
KEYWORDS    WGS; ENV; Metagenome Assembled Genome; MAG; Third Party Data; TPA;
            TPA:assembly.
SOURCE      Micropruina sp. (wastewater metagenome)
  ORGANISM  Micropruina sp.
            Bacteria; Bacillati; Actinomycetota; Actinomycetes;
            Propionibacteriales; Nocardioidaceae; Micropruina.
REFERENCE   1  (bases 1 to 3727)
  AUTHORS   Abdulkadir,N., Saraiva,J.P., Zhang,J., Stolte,S., Gillor,O.,
            Harms,H. and Rocha,U.
  TITLE     Genome-centric analyses of 165 metagenomes show that mobile genetic
            elements are crucial for the transmission of antimicrobial
            resistance genes to pathogens in activated sludge and wastewater
  JOURNAL   Microbiol Spectr 12 (3), e0291823 (2024)
   PUBMED   38289113
REFERENCE   2  (bases 1 to 3727)
  AUTHORS   Abdulkadir,N.
  TITLE     Direct Submission
  JOURNAL   Submitted (24-MAR-2023) Environmental Microbiology, Helmholtz
            Center for Environmental Research -UFZ, Permoserstr 15, Leipzig
            04318, Germany
COMMENT     The annotation was added by the NCBI Prokaryotic Genome Annotation
            Pipeline (PGAP). Information about PGAP can be found here:
            https://www.ncbi.nlm.nih.gov/genome/annotation_prok/
            
            ##Genome-Assembly-Data-START##
            Assembly Method        :: SPAdes v. 3.11.1
            Genome Representation  :: Full
            Expected Final Version :: Yes
            Genome Coverage        :: Not Applicable
            Sequencing Technology  :: Illumina MiSeq
            ##Genome-Assembly-Data-END##
            
            ##Genome-Annotation-Data-START##
            Annotation Provider               :: NCBI
            Annotation Date                   :: 05/04/2023 04:57:10
            Annotation Pipeline               :: NCBI Prokaryotic Genome
                                                 Annotation Pipeline (PGAP)
            Annotation Method                 :: Best-placed reference protein
                                                 set; GeneMarkS-2+
            Annotation Software revision      :: 6.5
            Features Annotated                :: Gene; CDS; rRNA; tRNA; ncRNA
            Genes (total)                     :: 2,776
            CDSs (total)                      :: 2,750
            Genes (coding)                    :: 2,714
            CDSs (with protein)               :: 2,714
            Genes (RNA)                       :: 26
            tRNAs                             :: 24
            ncRNAs                            :: 2
            Pseudo Genes (total)              :: 36
            CDSs (without protein)            :: 36
            Pseudo Genes (ambiguous residues) :: 0 of 36
            Pseudo Genes (frameshifted)       :: 2 of 36
            Pseudo Genes (incomplete)         :: 32 of 36
            Pseudo Genes (internal stop)      :: 2 of 36
            ##Genome-Annotation-Data-END##
FEATURES             Location/Qualifiers
     source          1..3727
                     /organism="Micropruina sp."
                     /mol_type="genomic DNA"
                     /submitter_seqid="NODE_10215_length_3727_cov_4.892429"
                     /isolate="AMR_MDS_2195"
                     /isolation_source="Activated sludge of municipal
                     wastewater treatment plants"
                     /db_xref="taxon:2737536"
                     /environmental_sample
                     /geo_loc_name="Canada"
                     /lat_lon="49.25 N 123.1 W"
                     /collection_date="2015-06-16"
                     /metagenome_source="wastewater metagenome"
                     /note="metagenomic"
     gene            complement(<1..642)
                     /locus_tag="PLF56_09960"
     CDS             complement(<1..642)
                     /locus_tag="PLF56_09960"
                     /inference="COORDINATES: ab initio
                     prediction:GeneMarkS-2+"
                     /note="Derived by automated computational analysis using
                     gene prediction method: GeneMarkS-2+."
                     /codon_start=1
                     /transl_table=11
                     /product="hypothetical protein"
                     /protein_id="HOQ53926.1"
                     /translation="MSDPTLPADPQHLDAGSVRATVVRLDARIRARFPARNLTRLPGQ
                     LIEVIDHLTERQDVWRTRRRWLIRACRVGIGLVVLGLLYALVLIAVQSSASSEPHGWD
                     WLSVFESLVNDAVFAGIAIYFLWALPERLQRHGDLATLQRLRSVAHVVDMHQLTKDPE
                     RLRSDFAVTSESVALGMSAIELANYLDYCSELLSMVGKTAALFGEHTDDRTTLA"
     gene            751..2115
                     /locus_tag="PLF56_09965"
     CDS             751..2115
                     /locus_tag="PLF56_09965"
                     /EC_number="1.1.1.-"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_016455825.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_function: GO:0016616 - oxidoreductase activity, acting
                     on the CH-OH group of donors, NAD or NADP as acceptor
                     [Evidence IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="mannitol dehydrogenase family protein"
                     /protein_id="HOQ53927.1"
                     /translation="MAARLSRTKDGRPTPPVRIVHLGLGNFFRAHQAWYTDRAPDADQ
                     WGIAAFSGRSIGIAAELQRQDNLYTLVTVGPRGNDYEVIGSLAATHTGTDIAALLGYF
                     TDPEISIVTMTITEAGYVRNERGDLDTDNPDVRSDIEVIKAGNLTAVRTAPGKLVAGL
                     LARRDASAGPITLICCDNLPDNGAMLERILGELTKQSVPDLADFLGEQVGFITTMVDR
                     ITPRATESTRQAVQDELGIDDPAAIATEPFSEWVLAGDFKSPRPAWHAVGAEFVDDVV
                     PHELRKLWLLNGAHSLMAYAATIAGHQTVSDAIADPVVRGWVDEWWDVAQRQLPLEPQ
                     IVSEYRAALLERFGNPRMRDQLSRIAADGSQKIPIRIVPALVADRKAGNSPLGAERAV
                     AAWILHLRGRGAPFNDAKADKIADLRTGTLEDSVRKTCEFLEIENRDSSASILALAQQ
                     LDAL"
     gene            2115..3641
                     /locus_tag="PLF56_09970"
     CDS             2115..3641
                     /locus_tag="PLF56_09970"
                     /EC_number="2.7.1.12"
                     /inference="COORDINATES: protein motif:HMM:NF012589.2"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_function: GO:0005524 - ATP binding [Evidence IEA];
                     GO_function: GO:0046316 - gluconokinase activity [Evidence
                     IEA];
                     GO_process: GO:0016310 - phosphorylation [Evidence IEA];
                     GO_process: GO:0046177 - D-gluconate catabolic process
                     [Evidence IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="gluconokinase"
                     /protein_id="HOQ53928.1"
                     /translation="MTAAASTPRVVIGLDVGTTAAKVSAFALGGGWRTSVSHEYPLQQ
                     PQPGWEVQDPMLVWRALHEGLADVATECGAAEIVGIGVSSAMHGLIGLDEELCPITPL
                     LTWADSRAADQAMRLRDTELGRRLHRTSGTPIHSMSHLTKLLWYGENEPETLRAARWW
                     VGLKDFLLYQLTGELVTEVSNASGTGLLDLATRTWNPEALALVGVGAERLPPVRPTTD
                     ALPLRDDLADALGLPRGLPVVLGAGDGPLGNLGVGALEPGVVGLSLGTSGAIRMITDR
                     PYVDESGKLFCYHLAQDSWVIGGAISNGGIVVRWAGDVFGVDFADEPGGNPDGLLLDL
                     ARDVPLGSDGLVMLPFLMAERAPLWDPTIPGAFLGVRRSHTRAHFVRAAVEGVALMLS
                     TIVDELDRVAPIEQIRATGGVFRSDVWREVLMGALGRPLVITDGAEGTALGAAALALL
                     GLGEVDSLRDGLVQLGQDPAAGLDAVVAEPADVAAYREVRAGIPALLERYQQVSDLFR
                     "
BASE COUNT          582 a         1259 c         1314 g          572 t
ORIGIN      
        1 ggccagggtg gtgcggtcgt cggtgtgttc gccgaacagg gccgcggtct tgccgaccat
       61 cgacaacaac tccgagcaat agtcgagata gttcgccagt tcgatcgcgc tcatgcccag
      121 cgccacgctc tcactggtga cggcgaagtc gctgcgcagt cgctcgggat ccttggtgag
      181 ctggtgcatg tcgacgacat gggccaccga ccgcagccgt tgcagcgtgg ccaggtcgcc
      241 gtgccgttgc agccgctcgg gcagcgccca cagaaagtag atcgcgatgc ccgcgaatac
      301 cgcgtcgttg accagcgact cgaacacgct cagccagtcc cagccgtgcg gctcgctcga
      361 tgccgacgac tgcaccgcga tcagcaccaa cgcatacagc agacccagca cgaccagccc
      421 gatgccgacc cggcaggcgc ggatcagcca gcgccgccgc gtgcgccaga cgtcctgccg
      481 ctcggtcagg tggtcgatca cttcgatcag ctgaccgggc agccgggtca ggttgcgggc
      541 cggaaaccgg gcccggatgc gggcatccag ccggacgacc gtggcgcgca ccgacccggc
      601 gtccaggtgt tgcgggtcgg ctggcagggt cgggtcggac acgccgcaca ccctactggc
      661 ccgtggtcgc gggcaaccga tggcaacccg tccgccgcgc gctggtcatg ctctaggctc
      721 gacagcgccc ctacgcgaaa ggcaggcgac gtggccgctc gactcagccg aaccaaggac
      781 ggacggccca cgccaccggt gcgaatcgtc catctgggcc tcggcaactt cttccgagcg
      841 catcaggcct ggtacaccga ccgggccccc gacgccgacc agtggggcat tgccgcgttc
      901 tcgggacgtt ccatcggcat cgccgcggaa ctgcagcgcc aggacaacct ctacaccctg
      961 gtgaccgtcg ggcctcgtgg caacgactac gaagtgatag gttcgctggc cgccacgcac
     1021 accggcaccg acatcgccgc gctgctgggc tatttcaccg atcccgagat cagcatcgtg
     1081 acgatgacga tcaccgaggc cggctacgtg cgcaacgaac gcggcgacct cgacaccgac
     1141 aatcccgacg tgcgctccga catcgaggtg atcaaggccg gcaacctgac ggcggtgcgt
     1201 accgcaccgg gcaaactggt cgccgggctg ctggcgcggc gcgacgcgag cgccggaccg
     1261 atcaccctga tctgttgcga caacctgccc gacaacggcg ccatgcttga gcgcatcctg
     1321 ggcgaactga ccaagcagtc cgtacccgac ctggccgact ttctcggcga gcaggtcggc
     1381 ttcatcacca ccatggtcga ccgcatcacc cccagggcga ccgaatcgac gcggcaggcg
     1441 gtgcaggacg aactgggcat cgacgacccc gcggcgatcg ccaccgagcc gttcagcgaa
     1501 tgggtgctgg ccggcgactt caagagcccg cgacccgcgt ggcacgcggt cggggccgag
     1561 ttcgtcgacg acgtcgtgcc ccacgagttg cgcaaactgt ggttgctcaa cggtgcccac
     1621 tcgttgatgg cgtacgcggc gaccatcgcc ggtcaccaaa ccgtgtcgga cgccatcgcc
     1681 gaccccgtag tgcggggttg ggtggacgaa tggtgggacg tcgcgcagcg tcaactgccg
     1741 ctggagccgc agattgtgag tgagtaccgg gccgcgctgc tggagcggtt cggcaacccc
     1801 cggatgcggg atcagctcag ccggatcgcg gccgacgggt cgcagaagat cccgatccgg
     1861 atcgtcccgg ccctggtcgc cgaccgcaag gccggcaaca gtccgctggg ggccgagcgg
     1921 gcggtggcgg cctggattct gcatctgcgg gggcgtggcg cgccgttcaa tgacgccaag
     1981 gccgacaaga tcgcagacct gcgaaccgga accctggagg attcggtacg caagacctgc
     2041 gagtttcttg agatcgagaa ccgggactcg tcggccagca tcctcgccct ggcccagcag
     2101 ttggacgctc tgtgatgacc gcagcagcat caacaccccg cgtggtgatc ggcctcgacg
     2161 tgggcaccac ggccgccaag gtgtcggcct tcgcgctagg tggcgggtgg cgcaccagcg
     2221 tttcgcacga gtatccgctg cagcagccgc aacccggctg ggaggtgcag gacccgatgc
     2281 tggtctggcg cgccctgcac gaggggctcg ccgatgttgc gaccgagtgc ggtgcggccg
     2341 agatcgtcgg catcggggtg tcgagcgcca tgcacggcct gatcgggctg gacgaggagc
     2401 tctgtccgat caccccgttg ctcacctggg cggactcgcg cgcggccgac caggcgatgc
     2461 ggttgcgcga tacggaactc ggacggcgtc ttcaccgcac ctccggcacg ccgattcact
     2521 cgatgtcgca cctgaccaag ctgctctggt acggcgagaa cgagcccgag acgctgcggg
     2581 cggcacgctg gtgggtcggg ttgaaggact tcctgctgta ccaactgacc ggcgagttgg
     2641 tgacagaggt gtccaacgcg tccggcaccg ggctgctcga cctggccacc cgcacctgga
     2701 atccggaggc gctggcactg gtcggggtgg gcgctgagcg actgcccccc gtccggccga
     2761 ccacggatgc gctgccccta cgcgacgacc tcgccgatgc gctcggcctg ccgcgcggac
     2821 tgccggtggt gttgggtgct ggtgacgggc cgttgggcaa tctgggcgtg ggcgccttgg
     2881 agcccggggt ggttgggctc tcgctcggca cgtccggagc gatccgcatg atcaccgacc
     2941 ggccctacgt ggacgagtcc ggcaagctgt tctgctacca cctggctcag gattcatggg
     3001 tgatcggcgg agcgatcagc aacggcggca tcgtggtgcg ctgggccggt gacgtgttcg
     3061 gcgtcgactt cgccgacgaa ccgggcggca accccgacgg actcctgctc gacctggccc
     3121 gcgacgtgcc gttggggtcg gacgggctgg tcatgctgcc gttcctgatg gccgagcggg
     3181 caccgctgtg ggatcccacg attccgggcg cgttcctcgg cgttcggcgt agtcataccc
     3241 gcgcgcattt cgtccgcgcg gcggtcgagg gggtggcgct gatgctctcg acgatcgtgg
     3301 acgaactcga tcgggtggcg ccgatcgagc agatccgggc caccggagga gtgttccgct
     3361 ccgacgtgtg gcgcgaggtg ctgatggggg cgctgggtag accgctggtg atcaccgacg
     3421 gcgccgaggg caccgcattg ggcgcggcgg cgctggccct gttggggctg ggtgaggtgg
     3481 actcgctgcg cgacgggctc gtgcagttgg gccaggaccc ggccgcaggg ttggacgcgg
     3541 tggtggccga accggccgac gtggcggcct accgcgaggt gcgagcaggc attccggcgc
     3601 tgctcgagcg ctaccagcag gtgagcgacc tgttccggtg accggtgccg aggtgccact
     3661 gatgagcctg gttccaccga tctgcgccta ctgcgcggcc ccatccgggc accctggctg
     3721 cgtcggc
//
LOCUS       DAOSUG010000304         3716 bp    DNA     linear   ENV 10-JUN-2024
DEFINITION  MAG TPA_asm: Micropruina sp. isolate AMR_MDS_2195
            NODE_10256_length_3716_cov_5.256487, whole genome shotgun sequence.
ACCESSION   DAOSUG010000304 DAOSUG010000000
VERSION     DAOSUG010000304.1
DBLINK      BioProject: PRJNA925477
            BioSample: SAMN32864262
            Sequence Read Archive: SRR4236673
KEYWORDS    WGS; ENV; Metagenome Assembled Genome; MAG; Third Party Data; TPA;
            TPA:assembly.
SOURCE      Micropruina sp. (wastewater metagenome)
  ORGANISM  Micropruina sp.
            Bacteria; Bacillati; Actinomycetota; Actinomycetes;
            Propionibacteriales; Nocardioidaceae; Micropruina.
REFERENCE   1  (bases 1 to 3716)
  AUTHORS   Abdulkadir,N., Saraiva,J.P., Zhang,J., Stolte,S., Gillor,O.,
            Harms,H. and Rocha,U.
  TITLE     Genome-centric analyses of 165 metagenomes show that mobile genetic
            elements are crucial for the transmission of antimicrobial
            resistance genes to pathogens in activated sludge and wastewater
  JOURNAL   Microbiol Spectr 12 (3), e0291823 (2024)
   PUBMED   38289113
REFERENCE   2  (bases 1 to 3716)
  AUTHORS   Abdulkadir,N.
  TITLE     Direct Submission
  JOURNAL   Submitted (24-MAR-2023) Environmental Microbiology, Helmholtz
            Center for Environmental Research -UFZ, Permoserstr 15, Leipzig
            04318, Germany
COMMENT     The annotation was added by the NCBI Prokaryotic Genome Annotation
            Pipeline (PGAP). Information about PGAP can be found here:
            https://www.ncbi.nlm.nih.gov/genome/annotation_prok/
            
            ##Genome-Assembly-Data-START##
            Assembly Method        :: SPAdes v. 3.11.1
            Genome Representation  :: Full
            Expected Final Version :: Yes
            Genome Coverage        :: Not Applicable
            Sequencing Technology  :: Illumina MiSeq
            ##Genome-Assembly-Data-END##
            
            ##Genome-Annotation-Data-START##
            Annotation Provider               :: NCBI
            Annotation Date                   :: 05/04/2023 04:57:10
            Annotation Pipeline               :: NCBI Prokaryotic Genome
                                                 Annotation Pipeline (PGAP)
            Annotation Method                 :: Best-placed reference protein
                                                 set; GeneMarkS-2+
            Annotation Software revision      :: 6.5
            Features Annotated                :: Gene; CDS; rRNA; tRNA; ncRNA
            Genes (total)                     :: 2,776
            CDSs (total)                      :: 2,750
            Genes (coding)                    :: 2,714
            CDSs (with protein)               :: 2,714
            Genes (RNA)                       :: 26
            tRNAs                             :: 24
            ncRNAs                            :: 2
            Pseudo Genes (total)              :: 36
            CDSs (without protein)            :: 36
            Pseudo Genes (ambiguous residues) :: 0 of 36
            Pseudo Genes (frameshifted)       :: 2 of 36
            Pseudo Genes (incomplete)         :: 32 of 36
            Pseudo Genes (internal stop)      :: 2 of 36
            ##Genome-Annotation-Data-END##
FEATURES             Location/Qualifiers
     source          1..3716
                     /organism="Micropruina sp."
                     /mol_type="genomic DNA"
                     /submitter_seqid="NODE_10256_length_3716_cov_5.256487"
                     /isolate="AMR_MDS_2195"
                     /isolation_source="Activated sludge of municipal
                     wastewater treatment plants"
                     /db_xref="taxon:2737536"
                     /environmental_sample
                     /geo_loc_name="Canada"
                     /lat_lon="49.25 N 123.1 W"
                     /collection_date="2015-06-16"
                     /metagenome_source="wastewater metagenome"
                     /note="metagenomic"
     gene            163..606
                     /locus_tag="PLF56_09975"
     CDS             163..606
                     /locus_tag="PLF56_09975"
                     /inference="COORDINATES: ab initio
                     prediction:GeneMarkS-2+"
                     /note="Derived by automated computational analysis using
                     gene prediction method: GeneMarkS-2+."
                     /codon_start=1
                     /transl_table=11
                     /product="hypothetical protein"
                     /protein_id="HOQ53929.1"
                     /translation="MRRLGLLAAMLLLSACAASTPASTPTSSDSPRSPTGRFQTTSPL
                     PTSSSPSGVPTTVPDARLAAIRGALTARGVTTDQLRVVSAESVTFNDGSLGCPTPGVQ
                     YTQAQVEGMRVVVEADARQFDFRFGRTDTPTLCENPGPRSTSSNR"
     gene            complement(989..1294)
                     /locus_tag="PLF56_09980"
     CDS             complement(989..1294)
                     /locus_tag="PLF56_09980"
                     /inference="COORDINATES: protein motif:HMM:NF021585.2"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="DUF2316 family protein"
                     /protein_id="HOQ53930.1"
                     /translation="MSLNDAERRRTADELQQALQHCGLSADVVATDLGFSRERLTGVL
                     NLNGEQNPVDVWQLRDYLQQAALDQGLTPTRFSVLTESARSAARQWFQLRAAPRHEF"
     gene            complement(1439..>3716)
                     /locus_tag="PLF56_09985"
     CDS             complement(1439..>3716)
                     /locus_tag="PLF56_09985"
                     /inference="COORDINATES: protein motif:HMM:NF012311.2"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_function: GO:0008236 - serine-type peptidase activity
                     [Evidence IEA];
                     GO_process: GO:0006508 - proteolysis [Evidence IEA]"
                     /codon_start=2
                     /transl_table=11
                     /product="S8 family serine peptidase"
                     /protein_id="HOQ53931.1"
                     /translation="AIDWAVKNKMQVINMSLGSTYGTADDSDSIAAQNATAAGVVVVA
                     SAGNSGPNPYLTGSPAAGRGVISVAAVDSTKSFPGATLSFDGKTLNAINANGLSPLPA
                     GPFTIVDTKSLGCSAAAFTAAGITPGGNQIAIVNRGTCARAAKAIFGQQAGAAAVIMV
                     NNATDYPPYEGPITSNPDDGTPYTVTIPFLGVRSTDGPALVAAAGKPLTIAAKDLDNP
                     AFKAYASFSSGGPRNGDSGLKPSVSAPGVSISSVAVGTGTGAEVLSGTSMASPHVAGV
                     AALARQAHKKWNAQQISAALVTTADPAKVAGYRLTLGGGLVDTKQVVKTSVFAYGDSY
                     KTKAGTVTEGTLSYGFSEPSGTYQGVKKLTLVNKGSKKATFLLSSKKTAQSRPATVSF
                     SKRVVTVKANSSATVKVTLKVKGSSVGSSIADVDDQFNFYEVSGNIKIATDGKGTLRV
                     PYLLVPRAQAKVAATQKTVKSTTVVTTEKPSEGATAAPAATTSPATTGTTSPAAAGTQ
                     ATTDGASSKTPATRFADVKLTNAKGALTAAADFYTWGLYDKKDVKKGTGFDLRAAGVQ
                     SFATDDDDQFMVFAVNNWTRWSNAATNEFDVSIDVNHDDQVDYVVFSADSGLVRSGDP
                     DGVAEVFLYDVAKDATYATGFLAQAPTDSSTILLPVYASQLGISSTTGAFDYSVASSS
                     SIDSSLSDEFYNKASYDPYHPAISNGHGAEVKVNKSQTVRLTVDTAQVNTQKPLGTMV
                     VVLDNKAGEKEALLLNKP"
BASE COUNT          524 a         1259 c         1251 g          682 t
ORIGIN      
        1 taaccctggc cgcgctgcgg cgcacccagt acgcccgctc gctgcgttgt catcctcggc
       61 gagaggaccc actatcgact cgtcttccgc cttgcgatca gacgcactgg gcacgtcctc
      121 gcggaacgcc cacggttatg cgacacgccc tagactgatc gcatgcgccg cctcggactg
      181 cttgccgcca tgctgctgct gagcgcctgc gccgcctcga cgccggccag cacgcccacg
      241 tcgtccgaca gtccgcggtc gcccaccggc aggtttcaga ccacctcgcc gctacccacc
      301 agctcgtccc cctcaggggt gccgaccacc gttccggacg ccaggctggc cgcgatccgc
      361 ggggctctga ccgcccgtgg cgtcaccact gatcaactgc gcgtggtcag cgccgagagt
      421 gtgaccttca acgacggctc gctgggctgc cccacgcccg gtgtgcagta cacccaggcc
      481 caggtcgagg gcatgcgggt cgttgtcgaa gccgatgcac gtcagttcga cttccggttc
      541 ggacgtaccg acaccccgac cctgtgcgag aaccccggac cccgctcgac ttcgtccaac
      601 cgctgatcac gaggtccgcc ccgaccggcg tgatcggacg cgacgacacc cctgcccgcg
      661 atgatcagcg gttgggaccc gccgtgggga acactcgccc aatggatcgg gcggggatca
      721 gcccttcctt ctacgtgtca tccggtgctt gccccgggat ctcggcgcag gtggagtttc
      781 cggcgtgcgc cgggatgacg ggggtcacgt tgtgtgcggg ggtcacgttg cgtacgtgtc
      841 atccggtgct tgccccggga tctcggcgca ggtggagttc ccggcgtgcg ccgggatgac
      901 ggggtcaagg gcccgcgggc gtgcgccggg atgactatgg gcacagcatt gatcagatct
      961 tgcttgggtg actccgaggt cggactgctc agaactcgtg ccgcggtgca gcgcgaagct
     1021 ggaaccactg ccgcgcagca ctgcgggccg attcggtgag cacgctgaac cgtgtgggcg
     1081 tcaggccctg atccagcgct gcctgctgca ggtagtcgcg caactgccac acgtccaccg
     1141 ggttctgctc cccgttcagg ttcagcaccc cggtcagccg ctcgcgcgaa aagcccaggt
     1201 cggtggcgac cacgtccgcg ctgagaccgc aatgctgcag agcctgctgc aactcatcgg
     1261 ccgtgcgtcg ccgctcagca tcgttcagtg acatcgttcc ccctctggac tccccggctg
     1321 tcaccacgct actcacgccc ctcgacccgg acgcgctcgg gtcgagccgg tggggatcag
     1381 gggccccgaa cgcgccgacg cccccgggcg aacccggggg cgtcggtcgt tgagcaggtc
     1441 agggcttgtt gagcagcaat gcctctttct cgcccgcctt gttgtcgagc acgacgacca
     1501 tcgtgcccag cggcttctgg gtgttgacct gagccgtgtc caccgtcagg cggacggtct
     1561 ggctcttgtt caccttgacc tcagcaccgt gtccgttgct gatcgccggg tggtacgggt
     1621 cgtagctcgc cttgttgtag aactcgtccg acagcgacga gtcgatcgac gaggagctgg
     1681 ccaccgagta gtcgaacgca cccgtggtcg agctgatgcc caactggctg gcgtagaccg
     1741 gcagcagaat ggtgctgctg tcggtcggtg cctgggccag gaagccggtc gcgtacgtgg
     1801 cgtccttggc cacgtcgtag aggaacacct cggcgacccc gtccgggtct ccggaccgca
     1861 ccaggccgga gtcggcgctg aacacgacgt aatccacctg gtcgtcgtgg ttcacgtcga
     1921 tgctcacgtc gaactcgttc gtggccgcgt tcgaccagcg cgtccagttg ttcaccgcga
     1981 acaccatgaa ctgatcgtca tcgtcagtgg cgaacgactg cacaccggct gcccgcaggt
     2041 cgaaaccggt gcccttcttg acgtccttct tgtcgtacag gccccaggtg tagaagtccg
     2101 ccgcggcggt cagcgcaccc ttggcgttgg tcagcttcac atcggcgaag cgggtggcgg
     2161 gcgtcttgga cgacgcgccg tcggtcgtgg cctgggtgcc ggctgcggcc ggcgacgtgg
     2221 ttccggttgt ggccggtgac gtcgtggcgg ccggtgccgc ggtggcgccc tcgctgggct
     2281 tctccgtggt caccaccgtc gtggacttga ccgtcttctg ggtcgccgcg accttcgcct
     2341 gggcgcgggg caccagcagg tacggcaccc gcagtgtgcc cttgccgtca gtggcgatct
     2401 tgatgttgcc cgacacctca taaaagttga actgatcgtc cacgtcggcg atcgagctgc
     2461 cgacggacga gcccttgacc ttgagcgtga ccttgaccgt tgcgctgctg ttggccttga
     2521 ccgtcacgac cctcttgctg aagctcaccg ttgcgggacg gctctgggcc gtcttcttgc
     2581 tgctgagcag gaaggtcgcc ttcttgctgc ccttgttgac tagcgtcagc ttcttgaccc
     2641 cctggtaggt gccactcggc tcgctgaagc cgtagctcag ggtgccctca gtcaccgtgc
     2701 cggccttggt cttgtagctg tcgccgtagg cgaagacgga ggtcttgacc acctgcttgg
     2761 tgtccaccag gccgccgccc agggtcagcc ggtagcccgc caccttggcc gggtcggccg
     2821 tggtgaccag cgccgcgctg atctgctgcg cattccactt cttgtgcgcc tggcgagcca
     2881 gcgccgccac acccgcgaca tgcggcgagg ccatcgaggt acccgagagc acctcggcgc
     2941 cggttccggt accgaccgcg accgaactga tgctcacgcc cggtgccgaa acggacggct
     3001 tgaggccgct gtcgccgttg cgggggccgc ccgaactgaa cgaggcgtac gccttgaacg
     3061 ccgggttgtc caggtccttg gcggctatgg tcaacggctt gccggccgcg gccaccaggg
     3121 ccggaccgtc ggtgctgcgc acgccgagga acggaatggt caccgtgtag ggggtgccgt
     3181 catcggggtt gctggtgatt ggaccctcgt acggcgggta gtcagtggca ttgttcacca
     3241 tgatcaccgc ggcagcgccg gcctgctggc cgaagatcgc cttggccgcc cgggcacagg
     3301 tgccccggtt gacgatggcg atctgattgc cgccgggggt gatgccggcc gcggtgaagg
     3361 ccgcggcgct gcaccccagc gatttggtgt cgacgatcgt gaacggaccg gcgggaagcg
     3421 gggacagccc gttggcgttg atcgcgttca gggtcttgcc gtcgaaactc agcgttgcgc
     3481 cgggaaagct cttggtcgag tcgaccgcgg cgacgctgat gacgccgcgg cccgcagcgg
     3541 gcgacccggt caggtagggg ttggggccgc tgttgccggc cgacgcgacg acgacgacgc
     3601 cggccgcggt ggcgttctgc gcggcgatcg aatcggagtc gtcagcggtg ccgtaggtcg
     3661 atcccagcga catgttgatg acctgcatct tgttcttgac ggcccagtcg atggcc
//
LOCUS       DAOSUG010000305         3701 bp    DNA     linear   ENV 10-JUN-2024
DEFINITION  MAG TPA_asm: Micropruina sp. isolate AMR_MDS_2195
            NODE_10301_length_3701_cov_4.390016, whole genome shotgun sequence.
ACCESSION   DAOSUG010000305 DAOSUG010000000
VERSION     DAOSUG010000305.1
DBLINK      BioProject: PRJNA925477
            BioSample: SAMN32864262
            Sequence Read Archive: SRR4236673
KEYWORDS    WGS; ENV; Metagenome Assembled Genome; MAG; Third Party Data; TPA;
            TPA:assembly.
SOURCE      Micropruina sp. (wastewater metagenome)
  ORGANISM  Micropruina sp.
            Bacteria; Bacillati; Actinomycetota; Actinomycetes;
            Propionibacteriales; Nocardioidaceae; Micropruina.
REFERENCE   1  (bases 1 to 3701)
  AUTHORS   Abdulkadir,N., Saraiva,J.P., Zhang,J., Stolte,S., Gillor,O.,
            Harms,H. and Rocha,U.
  TITLE     Genome-centric analyses of 165 metagenomes show that mobile genetic
            elements are crucial for the transmission of antimicrobial
            resistance genes to pathogens in activated sludge and wastewater
  JOURNAL   Microbiol Spectr 12 (3), e0291823 (2024)
   PUBMED   38289113
REFERENCE   2  (bases 1 to 3701)
  AUTHORS   Abdulkadir,N.
  TITLE     Direct Submission
  JOURNAL   Submitted (24-MAR-2023) Environmental Microbiology, Helmholtz
            Center for Environmental Research -UFZ, Permoserstr 15, Leipzig
            04318, Germany
COMMENT     The annotation was added by the NCBI Prokaryotic Genome Annotation
            Pipeline (PGAP). Information about PGAP can be found here:
            https://www.ncbi.nlm.nih.gov/genome/annotation_prok/
            
            ##Genome-Assembly-Data-START##
            Assembly Method        :: SPAdes v. 3.11.1
            Genome Representation  :: Full
            Expected Final Version :: Yes
            Genome Coverage        :: Not Applicable
            Sequencing Technology  :: Illumina MiSeq
            ##Genome-Assembly-Data-END##
            
            ##Genome-Annotation-Data-START##
            Annotation Provider               :: NCBI
            Annotation Date                   :: 05/04/2023 04:57:10
            Annotation Pipeline               :: NCBI Prokaryotic Genome
                                                 Annotation Pipeline (PGAP)
            Annotation Method                 :: Best-placed reference protein
                                                 set; GeneMarkS-2+
            Annotation Software revision      :: 6.5
            Features Annotated                :: Gene; CDS; rRNA; tRNA; ncRNA
            Genes (total)                     :: 2,776
            CDSs (total)                      :: 2,750
            Genes (coding)                    :: 2,714
            CDSs (with protein)               :: 2,714
            Genes (RNA)                       :: 26
            tRNAs                             :: 24
            ncRNAs                            :: 2
            Pseudo Genes (total)              :: 36
            CDSs (without protein)            :: 36
            Pseudo Genes (ambiguous residues) :: 0 of 36
            Pseudo Genes (frameshifted)       :: 2 of 36
            Pseudo Genes (incomplete)         :: 32 of 36
            Pseudo Genes (internal stop)      :: 2 of 36
            ##Genome-Annotation-Data-END##
FEATURES             Location/Qualifiers
     source          1..3701
                     /organism="Micropruina sp."
                     /mol_type="genomic DNA"
                     /submitter_seqid="NODE_10301_length_3701_cov_4.390016"
                     /isolate="AMR_MDS_2195"
                     /isolation_source="Activated sludge of municipal
                     wastewater treatment plants"
                     /db_xref="taxon:2737536"
                     /environmental_sample
                     /geo_loc_name="Canada"
                     /lat_lon="49.25 N 123.1 W"
                     /collection_date="2015-06-16"
                     /metagenome_source="wastewater metagenome"
                     /note="metagenomic"
     gene            complement(28..972)
                     /locus_tag="PLF56_09990"
     CDS             complement(28..972)
                     /locus_tag="PLF56_09990"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_014846481.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_function: GO:0042626 - ATPase-coupled transmembrane
                     transporter activity [Evidence IEA];
                     GO_function: GO:0140359 - ABC-type transporter activity
                     [Evidence IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="sugar ABC transporter permease"
                     /protein_id="HOQ53932.1"
                     /translation="MSTTTSVPKAPTARRRRAAASRPPGSTKELWTALVFILPALIGF
                     VVFYLIPTIRGFYFSMTQYNLLSNNPRWVGFANYQQIFRDPLFYNALSVTIEYVLINI
                     TFQTILAVLIAAMMQRLTQSTLLRGAMLLPWLISNVIAALVWFWLLDYQIGIVNQFMS
                     TVGLNRTAFFADEFWAIPTIAFVNVWRHVGYTALLVFAGMQTIPKYVYEAASIDGSTE
                     WTSFWRITMPLIRPVLAMVLVITVTGSFQVFDTVAVTTRGGPVNVTRVFQYYIYQKAF
                     GEGQFGYGSALSVILFLILGVLAALQLRLLRSGESDLA"
     gene            1171..2244
                     /locus_tag="PLF56_09995"
     CDS             1171..2244
                     /locus_tag="PLF56_09995"
                     /inference="COORDINATES: protein motif:HMM:NF012742.2"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_function: GO:0000976 - transcription cis-regulatory
                     region binding [Evidence IEA];
                     GO_function: GO:0003700 - DNA-binding transcription factor
                     activity [Evidence IEA];
                     GO_function: GO:0043565 - sequence-specific DNA binding
                     [Evidence IEA];
                     GO_process: GO:0006351 - DNA-templated transcription
                     [Evidence IEA];
                     GO_process: GO:0006355 - regulation of DNA-templated
                     transcription [Evidence IEA];
                     GO_process: GO:0045892 - negative regulation of
                     DNA-templated transcription [Evidence IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="LacI family DNA-binding transcriptional
                     regulator"
                     /protein_id="HOQ53933.1"
                     /translation="MTRSNSRTHGAAVRDARPVTRADVARLAGVSSAVVSYVVNDGPR
                     PVAPATAQRVREAMELLGYRPNANARALRTGTTDTIGLVLGDSRNPFFTEYTFELVKG
                     AAARGYHVLISDAQQYGQGDPAALVDALVARQVDGLLFAAPHRGWVRGLSAASDVPFV
                     LVDAPGALPGVCSVGSAARAGAAEVTAHLLEHGRRHIALLIGEEGFGDPDPRDAGWLS
                     AHLEAGVRPGPQMRTTFTREGGHLGTLRLLQGNEPVDAIFATNDLQAIGALRALREQG
                     CSVPGDIALVSYDGTVEAEFAWPPLTVARQNVQAMASAALDLLAEPQPSAGRHIEIAT
                     QLILRASCGCPATTAAQAPEHES"
     gene            2249..>3701
                     /locus_tag="PLF56_10000"
     CDS             2249..>3701
                     /locus_tag="PLF56_10000"
                     /EC_number="3.2.1.22"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_013864233.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="alpha-galactosidase"
                     /protein_id="HOQ53934.1"
                     /translation="MPDHLDTRILLRAEGVALLLDLSAGQLPAVLHWGADLGELSADE
                     AAVIAESTVHPIVPNVVDEPVRVALLPEHRTGWVGRPGLSGSRSGAAWSPLWRTTRLR
                     IDGEPWAGDEPLISLGAAELEVSAADAEAGLDLTLAIELTPAGLVRARATLTNRGESD
                     YQLAGLVLALPVPPVTRELLDFGGRWGRERSPQRRAFTVGTHLREGRKGRTGPDAATV
                     LHAGVPGFGFAAGEVWGVHVAWSGNHTHYAERLCTGEQVLGGGELLLPGEITLATEES
                     YQGPWLYGSYGEGLDAVARRFHRQLRARPQHPSVERPVTINVWEAVYFDHTLQPLLEL
                     AEKAAAVGVERYVLDDGWFGARRNDRAGLGDWTVSKDVWPHGLHPLVNRVRELGMQFG
                     LWFEPEMINPDSDVARAHPEWIMSTGGRLPVPSRTQQVINLGIPACYEYVRDAILAIV
                     AEYHIDYIKWDHNRDLIDAGTAPSGRAGVHEQTL"
BASE COUNT          600 a         1256 c         1282 g          563 t
ORIGIN      
        1 cgatggttgt cattgccgta gctcctttta ggccaggtcg gattcgccgg agcgcagcag
       61 ccgcaactgc aacgcggcca gtacgccgag aatgaggaag aggatcaccg agagggcgct
      121 gccgtagccg aactgccctt caccgaaagc cttttggtag atgtagtact gaaacacccg
      181 ggtgacgttg acggggccgc cgcgagtggt caccgccacg gtgtcgaaca cctggaacga
      241 gccggtcacg gtgatcacca gcaccatcgc cagcactgga cggataagcg gcatggtgat
      301 gcgccagaac gacgtccatt cggtgctgcc gtcgatcgag gcggcctcgt acacatattt
      361 cggaatggtc tgcatgccgg cgaacaccag gagggcggtg tagccgacgt gccgccagac
      421 gttgacgaac gcgatggtcg ggatcgccca gaactcgtcg gcgaagaacg ccgtgcggtt
      481 caggccgacc gtgctcatga actggttgac gatgccgatc tggtagtcga gcagccagaa
      541 ccagaccagc gcggcgatca cgttcgagat cagccacggc agcagcatcg cgccgcgcag
      601 cagcgtcgac tgggtcagcc gctgcatcat cgccgcgatc aacaccgcca gaatcgtctg
      661 gaaggtgatg ttgatcagga cgtactcgat cgtcaccgac aacgcgttgt agaacagcgg
      721 atcgcgaaag atctgctggt agttggcgaa gccgacccag cgcgggttgt tgctgagcag
      781 gttgtactgc gtcatcgaga agtagaagcc gcggatggtc gggatcaggt agaacaccac
      841 gaacccgatc agcgccggca ggatgaacac cagcgccgtc cacaactcct tggtgctgcc
      901 cggtggacgg ctggccgccg ctcgccgccg gcgtgctgtc ggcgccttcg gcacggatgt
      961 tgtcgtactc accaactctc ccttcattgg gatcgaggac gccccggtgg cggcctctgg
     1021 tgatctgatg gcaggacgct acacgagtag atctttatgc gcaacaggtc gggccagatc
     1081 atttttcgag gctgaccgac cagtcagagg gggtgcgtat cccacgcaat aggtgtagat
     1141 tccagatcct gccatcgacg aggagaggcg atgacgcggt ccaactcacg cacccacggc
     1201 gccgccgtgc gcgacgcccg cccggtcacc cgtgccgacg tggcacgtct ggccggcgtc
     1261 agttctgccg tggtcagcta tgtcgtcaac gacggccccc gtccggtcgc gccggccacc
     1321 gcgcagcgcg tccgcgaggc gatggaactg ctcggctacc ggccaaacgc caatgcgcgg
     1381 gcgctgcgca ccggcaccac cgacaccatc ggcctggtgc tgggcgacag ccgcaacccg
     1441 ttcttcaccg agtacacctt cgagttggtg aagggcgccg cggcgcgtgg ctatcacgtg
     1501 ctgatcagcg atgcgcagca atacggccag ggcgaccccg ccgcgctggt ggacgccctg
     1561 gtcgcacgcc aggtcgacgg gttgctgttc gcggctccgc accgcgggtg ggtgcggggg
     1621 ctgtcggcgg cctcggacgt accgttcgtg ctggtcgatg cgccaggagc cctgcccggc
     1681 gtctgctcgg tcggctcggc cgcgcgggcc ggcgccgccg aggtgaccgc ccacctgctc
     1741 gagcacggcc gtcgtcacat cgctctgctg atcggcgagg agggcttcgg cgatccggat
     1801 ccgcgcgatg cgggctggct gagcgcgcac ctcgaagccg gtgtgcggcc cggcccacag
     1861 atgcgcacca ccttcacgcg tgagggcggc cacctcggca cccttcggct actgcagggg
     1921 aacgagccgg tcgacgccat cttcgccacc aacgacctgc aggcgatcgg cgcactgcgg
     1981 gcgttgcgcg agcaggggtg ttccgtaccc ggcgacatcg ccctggtcag ctatgacggc
     2041 accgtcgagg ccgagttcgc ctggccaccg ctgaccgtgg cgcggcagaa cgtgcaggcg
     2101 atggcgagcg ccgcgctcga cctgctcgcc gagccgcagc cgagtgccgg ccgacatatc
     2161 gagatcgcga cccaattgat cctccgggcg tcctgtggtt gcccggccac caccgccgcg
     2221 caggcgccag aacacgaaag ctgaatccat gccggaccat ctcgacaccc gaatcctgct
     2281 gcgcgccgaa ggcgtggccc tgctgctgga cctcagtgcc gggcagttgc cggcggtgct
     2341 gcactggggg gccgatctcg gcgagttgag cgccgacgag gccgcggtga tcgccgagtc
     2401 caccgtgcac ccgatcgtgc ccaacgtcgt ggacgagccc gtccgggttg cgttgttgcc
     2461 cgagcatcga accgggtggg tggggcgtcc cgggctcagc ggctcgcgca gcggcgccgc
     2521 ctggtcgcca ctgtggcgta ccacccgcct gcgcatcgac ggcgagccgt gggcgggcga
     2581 cgagcctctg atcagccttg gggccgccga gctcgaggtc tcggcggccg acgctgaggc
     2641 cggtctcgac ctcaccctcg ccatcgaact gactcccgcg gggctcgtcc gggctcgggc
     2701 gacgctcacc aaccgcggcg agtccgacta tcagctcgcc ggcctggtgc tggccctgcc
     2761 ggtgccgccg gtgacccgcg agctgctcga cttcggcggt cggtggggcc gcgagcgctc
     2821 gccgcagcgc cgcgccttca ccgtcggcac gcacctgcgc gaggggcgca agggccgcac
     2881 cggccccgac gcggcgaccg tactgcacgc gggcgtgccc ggcttcggct tcgcggccgg
     2941 cgaggtctgg ggagtgcacg tcgcctggag cggaaaccac acccactacg ccgaacgcct
     3001 ctgcaccggc gagcaggtgc tgggcggcgg cgagttgctg ctgccgggcg agatcaccct
     3061 ggcaacggaa gagagttatc agggtccgtg gctctacggc tcgtacggcg agggcctgga
     3121 cgcggtcgcc cgccgcttcc accgccagct ccgggcccgc ccgcagcacc cgtcggtgga
     3181 gcgtccggtg accatcaacg tgtgggaggc ggtctacttc gaccacaccc tgcagccgct
     3241 gctcgaactc gcagagaagg cggcggcagt aggggtcgag cgctacgtgc tggacgacgg
     3301 gtggttcggc gcccgccgaa acgaccgcgc gggtctgggc gactggaccg tgtcgaaaga
     3361 cgtgtggccg cacggcctgc acccgctggt gaaccgggtg cgcgagttgg gcatgcagtt
     3421 cgggctgtgg ttcgagcccg agatgatcaa ccccgactcg gacgtggccc gcgcgcatcc
     3481 cgagtggatc atgagcaccg gcgggcggct gccggtgccg tcacgcaccc agcaggtgat
     3541 caacctgggc attcccgcgt gctatgaata cgtgcgcgac gcgatcctcg ccatcgtggc
     3601 cgaataccac atcgactaca tcaagtggga ccacaaccgc gacctgatcg atgccggcac
     3661 cgcgccgtcc ggacgggcgg gggtgcacga gcagacgctc g
//
LOCUS       DAOSUG010000306         3698 bp    DNA     linear   ENV 10-JUN-2024
DEFINITION  MAG TPA_asm: Micropruina sp. isolate AMR_MDS_2195
            NODE_10314_length_3698_cov_3.488883, whole genome shotgun sequence.
ACCESSION   DAOSUG010000306 DAOSUG010000000
VERSION     DAOSUG010000306.1
DBLINK      BioProject: PRJNA925477
            BioSample: SAMN32864262
            Sequence Read Archive: SRR4236673
KEYWORDS    WGS; ENV; Metagenome Assembled Genome; MAG; Third Party Data; TPA;
            TPA:assembly.
SOURCE      Micropruina sp. (wastewater metagenome)
  ORGANISM  Micropruina sp.
            Bacteria; Bacillati; Actinomycetota; Actinomycetes;
            Propionibacteriales; Nocardioidaceae; Micropruina.
REFERENCE   1  (bases 1 to 3698)
  AUTHORS   Abdulkadir,N., Saraiva,J.P., Zhang,J., Stolte,S., Gillor,O.,
            Harms,H. and Rocha,U.
  TITLE     Genome-centric analyses of 165 metagenomes show that mobile genetic
            elements are crucial for the transmission of antimicrobial
            resistance genes to pathogens in activated sludge and wastewater
  JOURNAL   Microbiol Spectr 12 (3), e0291823 (2024)
   PUBMED   38289113
REFERENCE   2  (bases 1 to 3698)
  AUTHORS   Abdulkadir,N.
  TITLE     Direct Submission
  JOURNAL   Submitted (24-MAR-2023) Environmental Microbiology, Helmholtz
            Center for Environmental Research -UFZ, Permoserstr 15, Leipzig
            04318, Germany
COMMENT     The annotation was added by the NCBI Prokaryotic Genome Annotation
            Pipeline (PGAP). Information about PGAP can be found here:
            https://www.ncbi.nlm.nih.gov/genome/annotation_prok/
            
            ##Genome-Assembly-Data-START##
            Assembly Method        :: SPAdes v. 3.11.1
            Genome Representation  :: Full
            Expected Final Version :: Yes
            Genome Coverage        :: Not Applicable
            Sequencing Technology  :: Illumina MiSeq
            ##Genome-Assembly-Data-END##
            
            ##Genome-Annotation-Data-START##
            Annotation Provider               :: NCBI
            Annotation Date                   :: 05/04/2023 04:57:10
            Annotation Pipeline               :: NCBI Prokaryotic Genome
                                                 Annotation Pipeline (PGAP)
            Annotation Method                 :: Best-placed reference protein
                                                 set; GeneMarkS-2+
            Annotation Software revision      :: 6.5
            Features Annotated                :: Gene; CDS; rRNA; tRNA; ncRNA
            Genes (total)                     :: 2,776
            CDSs (total)                      :: 2,750
            Genes (coding)                    :: 2,714
            CDSs (with protein)               :: 2,714
            Genes (RNA)                       :: 26
            tRNAs                             :: 24
            ncRNAs                            :: 2
            Pseudo Genes (total)              :: 36
            CDSs (without protein)            :: 36
            Pseudo Genes (ambiguous residues) :: 0 of 36
            Pseudo Genes (frameshifted)       :: 2 of 36
            Pseudo Genes (incomplete)         :: 32 of 36
            Pseudo Genes (internal stop)      :: 2 of 36
            ##Genome-Annotation-Data-END##
FEATURES             Location/Qualifiers
     source          1..3698
                     /organism="Micropruina sp."
                     /mol_type="genomic DNA"
                     /submitter_seqid="NODE_10314_length_3698_cov_3.488883"
                     /isolate="AMR_MDS_2195"
                     /isolation_source="Activated sludge of municipal
                     wastewater treatment plants"
                     /db_xref="taxon:2737536"
                     /environmental_sample
                     /geo_loc_name="Canada"
                     /lat_lon="49.25 N 123.1 W"
                     /collection_date="2015-06-16"
                     /metagenome_source="wastewater metagenome"
                     /note="metagenomic"
     gene            complement(226..900)
                     /locus_tag="PLF56_10005"
                     /pseudo
     CDS             complement(226..900)
                     /locus_tag="PLF56_10005"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_002543226.1"
                     /note="incomplete; partial in the middle of a contig;
                     missing C-terminus; Derived by automated computational
                     analysis using gene prediction method: Protein Homology.
                     GO_function: GO:0003723 - RNA binding [Evidence IEA];
                     GO_function: GO:0004826 - phenylalanine-tRNA ligase
                     activity [Evidence IEA]"
                     /pseudo
                     /codon_start=1
                     /transl_table=11
                     /product="phenylalanine--tRNA ligase beta subunit-related
                     protein"
     gene            complement(979..1054)
                     /locus_tag="PLF56_10010"
     tRNA            complement(979..1054)
                     /locus_tag="PLF56_10010"
                     /product="tRNA-Glu"
                     /inference="COORDINATES: profile:tRNAscan-SE:2.0.12"
                     /note="Derived by automated computational analysis using
                     gene prediction method: tRNAscan-SE."
                     /anticodon=(pos:complement(1018..1020),aa:Glu,seq:ctc)
     gene            complement(1124..1195)
                     /locus_tag="PLF56_10015"
     tRNA            complement(1124..1195)
                     /locus_tag="PLF56_10015"
                     /product="tRNA-Gln"
                     /inference="COORDINATES: profile:tRNAscan-SE:2.0.12"
                     /note="Derived by automated computational analysis using
                     gene prediction method: tRNAscan-SE."
                     /anticodon=(pos:complement(1160..1162),aa:Gln,seq:ctg)
     gene            complement(1264..2247)
                     /locus_tag="PLF56_10020"
     CDS             complement(1264..2247)
                     /locus_tag="PLF56_10020"
                     /EC_number="3.4.-.-"
                     /inference="COORDINATES: protein motif:HMM:NF014566.2"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_component: GO:0016020 - membrane [Evidence IEA];
                     GO_function: GO:0004222 - metalloendopeptidase activity
                     [Evidence IEA];
                     GO_process: GO:0071586 - CAAX-box protein processing
                     [Evidence IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="CPBP family intramembrane metalloprotease"
                     /protein_id="HOQ53935.1"
                     /translation="MANQPAVTWVDRIATEPTPPGVRYDEILLGAEDRPKLRSSIGIV
                     TALLLFFVFAAAVAQLLNWVGWLVLRPDGTYTDWLRACAAFANPWGMLSGHLALATLI
                     PISMAVVLVVHRRHPDWLASVRPWFRWRYLVLSMVLALVVFNLFLFLQHLGQPFPTLQ
                     PQPNYWIFLVVILLTSPLQAAAEEFFFRGYLLQSFHTLAPRSPWFGVVCAAALFALFH
                     GTQNIPLFLDRFLFGVMVGVLVVKTGGLEAAIGAHVINNVLAFSYAGLYSTIAQVKAT
                     QTIGWVDLGWDLAGFGTYTVLAILIANRLKLATTTPGTRAPESVDPWALRT"
     gene            complement(2249..3052)
                     /locus_tag="PLF56_10025"
     CDS             complement(2249..3052)
                     /locus_tag="PLF56_10025"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_016455679.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_function: GO:0003824 - catalytic activity [Evidence
                     IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="fumarylacetoacetate hydrolase family protein"
                     /protein_id="HOQ53936.1"
                     /translation="MRIARFVADGGDPQYGIVELADDGGGHPDTVAAITGDPLAGPVN
                     YTGERVPLTEVRLVAPVIPRSKVVAIGKNYAAHAAEFNSSVPQEPLTFLKPNTSVIGP
                     GEPIVYPPETENLHFEGELAVVIGRICRRVPRERAAEVIFGYTVANDVTCRDLQARDK
                     QWTRSKSYDTFCPLGPWIVTHLSLDEVGELSIGTVLNGEQQQASRTSLMVRGVAELIE
                     FVSGFTTLLPGDVILTGTPEGVGPMQPGDEVSVTIESIGTLTNPVVAEG"
     gene            3473..>3698
                     /locus_tag="PLF56_10030"
     CDS             3473..>3698
                     /locus_tag="PLF56_10030"
                     /inference="COORDINATES: ab initio
                     prediction:GeneMarkS-2+"
                     /note="Derived by automated computational analysis using
                     gene prediction method: GeneMarkS-2+."
                     /codon_start=1
                     /transl_table=11
                     /product="isoamylase early set domain-containing protein"
                     /protein_id="HOQ53937.1"
                     /translation="MLKLTGSTKSTTRKITFALPADHPAGKVSVVGNFNNWTPGTDVL
                     KKRSNGTMSATVTVPADYIVAFRYLGENDAW"
BASE COUNT          677 a         1245 c         1211 g          565 t
ORIGIN      
        1 atgaagttga gcgacatcga ctcaaggctt ggcgggcgtc ttttgtttcc gcgttcaccg
       61 tcggcgaaac atgcaccacg tcgaacgcct tctccgagtc cacgaccgcc atctttggga
      121 gccccccgaa cgactcgcag gcgacactgc agctcagctg ggcagccgaa cgttgccggc
      181 gccaaggacg cggacggcga ccgacagttc cggttcgaga ccgcgcaacc ggtcgatcaa
      241 ctcgtccccg gcggcggtca gggcttcgtc ggtcatcggg gagagggcgt ccagaatgaa
      301 cagggcgctg gttgttgcgg gcgtgagttg cgttcggcga ccttggcgcc agttccagcg
      361 gcggcaggtc acaccgccgt catcgcacca gatcacctcg ccagggtccg gacgttcgac
      421 agccaccgcc ccgttggcca tggtgtcgaa cggttccgag cccgtggccc gcccgagtcg
      481 cggagggccg acgtaggcgc tcaggtcttc gccgccgatc ggcacctgat gcagcacact
      541 gatggcgttg tagatgtcgg tgaacggatt gaccctgggc agtcccgact ctgcgcggcg
      601 cagcagcgcc tcgaggctgt tgcgggtgcg ctgtggcttg gcgccgaact ggcggtacgc
      661 gtctcgccac gccgcgacgt ggggcaactg ttcgaccggg ttctcggcaa gcaactcgcg
      721 agccaccgtc tcggcaccaa gcaacagctc gtccgcagcc gcgtcgatgg gtccgccgcc
      781 ggtgctggcc gaacgcggca gcccagacac cccgatcagc agggcgcgat agtcaggacg
      841 cagggcgaag accgcctggt cgacactcgc cgaggccaga aaggcttcga gatcggtcat
      901 gagcgggccc tctcgtcagt gtcaaaacgg gccggaacga cgtcagccca gtcaatgttc
      961 gtctgactgg gctgaaggtg gcagccccga cgggattcga acccgcgcta ccgccttgag
     1021 agggcggcgt gctaggccgc tacacaacgg ggccctagct gtcgctttcg cgacccagcg
     1081 aacactacca aactgcttcc agcggcggcg aatctcgctg cagctggggt actaggactc
     1141 gaacctagac tgacggaacc agaatccggc gggctgccaa ttaccccata ccccactgcc
     1201 ggccctgtcg gaccagcgaa gagctacgtt accgaatcgg cgccgccggg gccaaatcgc
     1261 gcttcaggtg cgcaacgccc aggggtcgac actctcggga gcgcgggtac ccggggtcgt
     1321 ggtggccagt ttcaaccggt tggcgatcag gatcgccagc accgtgtagg tgccgaaccc
     1381 cgccagatcc caccccaggt ccacccatcc gatcgtctgg gtcgccttga cctgcgcgat
     1441 ggtcgagtag aggccggcgt agctgaacgc cagaacgttg ttgatcacgt gtgcgccgat
     1501 cgcggcctcg agtccgcccg tcttgaccac cagcacaccc accatcaccc cgaacagaaa
     1561 gcgatcgaga aacagcggaa tgttctgggt gccgtgaaac aacgcgaaca gggcggcggc
     1621 gcagaccacc ccgaaccacg ggctgcgcgg cgcgagcgtg tggaacgact gcaggaggta
     1681 gccgcgaaag aagaactctt ccgccgccgc ctgcagcggc gaggtgagca ggatcaccac
     1741 caggaagatc cagtagttcg gctgcggctg cagggtcggg aacggctgcc cgaggtgctg
     1801 cagaaacagg aacaggttga acaccaccaa cgccagcacc atgctgagca ccaggtagcg
     1861 ccagcggaac catggccgca ccgacgccag ccaatccgga tgccgtcgat gcacgaccaa
     1921 caccaccgcc atgctgatcg gtatcagggt ggccagggcc agatgtcccg acagcatgcc
     1981 ccacgggttc gcgaatgcgg cacacgcccg cagccaatcg gtataggtgc cgtccggccg
     2041 cagcaccagc cacccgaccc agttcagcag ctgcgcgacc gccgcggcga acacgaagaa
     2101 cagcagcaat gccgtgacga tgccgatgga cgaacgcagc ttgggccggt cctccgcgcc
     2161 gagcaggatc tcgtcgtagc gcacaccggg gggagtcggc tccgtcgcga tccggtccac
     2221 ccaggtgacg gcgggctgat tcgccacctc agccctccgc gaccacgggg ttggtcaggg
     2281 tgccgatcga ctcgatggtg accgacacct cgtcgcccgg ctgcatcggc ccgacgccct
     2341 cgggcgttcc ggtgagaatg acgtcgccgg gcaacagcgt ggtgaagccg ctgacgaact
     2401 cgatcagctc tgccaccccg cgcaccatca gcgaggtgcg ggaggcctgc tgctgttccc
     2461 cgttcaggac ggtgccgatg ctcagctcgc ccacctcgtc caagctcagg tgggtgacga
     2521 tccacgggcc gagcgggcag aacgtgtcgt agctcttgga gcgcgtccac tgcttgtcac
     2581 gtgcctgcag gtcgcggcag gtgacgtcgt tggcgaccgt gtagccgaag atcacctcgg
     2641 ccgcccgctc gcgcggcacc cggcggcaga tccgtccgat gaccacggcg agctcgccct
     2701 cgaagtgcag gttctcggtc tcgggcgggt acacgatcgg ttcacccgga ccgatcaccg
     2761 aggtgttcgg tttgaggaag gtgagtggct cctgcggcac ggaggagttg aactcggcgg
     2821 cgtgggcggc gtagttcttg ccgatcgcca ccaccttgct gcgcggaatc accggggcga
     2881 ccaggcgcac ctcggtgagc ggcacccgct caccggtgta gttgaccggg ccggccagcg
     2941 ggtcgccggt gatggccgcg accgtgtcgg ggtgcccgcc cccgtcgtcc gcgagttcga
     3001 cgatgccgta ctgagggtcg ccaccatcgg cgacgaaacg ggcgatgcgc atgccagcag
     3061 cctaaccggt gccgtatggc tggcacgctc cacccgccgt tgccacgctc ggcgccatgg
     3121 tcgccaccgg tgagcggtcg gacttcggct cgttgccccg tccggtggtg gacaagcact
     3181 cggttggtga gcctgtggac agctgtgccc atgagggagg cgacacgccc gtgctgatgg
     3241 ggtcccggcc agcgatggat cggcttcaac acgatagatg agcgtgcggc ggccggagca
     3301 aagcgaccgc aatcgggtaa ccatagcggc gcccgagcgc gctgcacggc tcgacacgct
     3361 cgaaatagtc acatccacta atagctggca acatggcatg aggcagactt cctcccgctt
     3421 cgtcggtgcc gtgctgaccc aaacctgatg tgaaaggctc aaagaagttc ccatgctgaa
     3481 actcaccgga tccaccaagt ccaccacccg caagatcacc ttcgcgctgc ccgccgatca
     3541 tccggccggc aaggtgtccg tggtgggcaa cttcaacaac tggaccccgg gaacggacgt
     3601 gctgaagaag cgctccaacg gcaccatgag cgccaccgtc actgtgcccg ccgactacat
     3661 cgtggcgttc cgctacctgg gtgagaacga cgcctggt
//
LOCUS       DAOSUG010000307         3692 bp    DNA     linear   ENV 10-JUN-2024
DEFINITION  MAG TPA_asm: Micropruina sp. isolate AMR_MDS_2195
            NODE_10342_length_3692_cov_4.300247, whole genome shotgun sequence.
ACCESSION   DAOSUG010000307 DAOSUG010000000
VERSION     DAOSUG010000307.1
DBLINK      BioProject: PRJNA925477
            BioSample: SAMN32864262
            Sequence Read Archive: SRR4236673
KEYWORDS    WGS; ENV; Metagenome Assembled Genome; MAG; Third Party Data; TPA;
            TPA:assembly.
SOURCE      Micropruina sp. (wastewater metagenome)
  ORGANISM  Micropruina sp.
            Bacteria; Bacillati; Actinomycetota; Actinomycetes;
            Propionibacteriales; Nocardioidaceae; Micropruina.
REFERENCE   1  (bases 1 to 3692)
  AUTHORS   Abdulkadir,N., Saraiva,J.P., Zhang,J., Stolte,S., Gillor,O.,
            Harms,H. and Rocha,U.
  TITLE     Genome-centric analyses of 165 metagenomes show that mobile genetic
            elements are crucial for the transmission of antimicrobial
            resistance genes to pathogens in activated sludge and wastewater
  JOURNAL   Microbiol Spectr 12 (3), e0291823 (2024)
   PUBMED   38289113
REFERENCE   2  (bases 1 to 3692)
  AUTHORS   Abdulkadir,N.
  TITLE     Direct Submission
  JOURNAL   Submitted (24-MAR-2023) Environmental Microbiology, Helmholtz
            Center for Environmental Research -UFZ, Permoserstr 15, Leipzig
            04318, Germany
COMMENT     The annotation was added by the NCBI Prokaryotic Genome Annotation
            Pipeline (PGAP). Information about PGAP can be found here:
            https://www.ncbi.nlm.nih.gov/genome/annotation_prok/
            
            ##Genome-Assembly-Data-START##
            Assembly Method        :: SPAdes v. 3.11.1
            Genome Representation  :: Full
            Expected Final Version :: Yes
            Genome Coverage        :: Not Applicable
            Sequencing Technology  :: Illumina MiSeq
            ##Genome-Assembly-Data-END##
            
            ##Genome-Annotation-Data-START##
            Annotation Provider               :: NCBI
            Annotation Date                   :: 05/04/2023 04:57:10
            Annotation Pipeline               :: NCBI Prokaryotic Genome
                                                 Annotation Pipeline (PGAP)
            Annotation Method                 :: Best-placed reference protein
                                                 set; GeneMarkS-2+
            Annotation Software revision      :: 6.5
            Features Annotated                :: Gene; CDS; rRNA; tRNA; ncRNA
            Genes (total)                     :: 2,776
            CDSs (total)                      :: 2,750
            Genes (coding)                    :: 2,714
            CDSs (with protein)               :: 2,714
            Genes (RNA)                       :: 26
            tRNAs                             :: 24
            ncRNAs                            :: 2
            Pseudo Genes (total)              :: 36
            CDSs (without protein)            :: 36
            Pseudo Genes (ambiguous residues) :: 0 of 36
            Pseudo Genes (frameshifted)       :: 2 of 36
            Pseudo Genes (incomplete)         :: 32 of 36
            Pseudo Genes (internal stop)      :: 2 of 36
            ##Genome-Annotation-Data-END##
FEATURES             Location/Qualifiers
     source          1..3692
                     /organism="Micropruina sp."
                     /mol_type="genomic DNA"
                     /submitter_seqid="NODE_10342_length_3692_cov_4.300247"
                     /isolate="AMR_MDS_2195"
                     /isolation_source="Activated sludge of municipal
                     wastewater treatment plants"
                     /db_xref="taxon:2737536"
                     /environmental_sample
                     /geo_loc_name="Canada"
                     /lat_lon="49.25 N 123.1 W"
                     /collection_date="2015-06-16"
                     /metagenome_source="wastewater metagenome"
                     /note="metagenomic"
     gene            <1..723
                     /locus_tag="PLF56_10035"
     CDS             <1..723
                     /locus_tag="PLF56_10035"
                     /EC_number="3.4.24.-"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_006213368.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_function: GO:0004222 - metalloendopeptidase activity
                     [Evidence IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="M23 family metallopeptidase"
                     /protein_id="HOQ53938.1"
                     /translation="GQTVATAYAHMWEHGIHVQAGDTVTAGQHIGDIGSSGNSTGPHL
                     HFEVSLGGTDGEHTDPAAWLNSHDVADLPEPETGTPGQECDPDTGGAPGGAPEPFEGD
                     PNQTVDDPTSGGQITARLLHLYTQTIAAFPDTSWVCYSPRPGTVSEHPLGRACDGTFG
                     NAIGSQPTPAQRELGWEVTNWMKDHAEALGVEYLIWDGRIWSISRDAEGWRPYDGGGM
                     HDPGDVTGGHYDHLHVTVSQNP"
     gene            1000..1266
                     /locus_tag="PLF56_10040"
     CDS             1000..1266
                     /locus_tag="PLF56_10040"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_013601305.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="DUF6112 family protein"
                     /protein_id="HOQ53939.1"
                     /translation="MNVFPDFDGLGGIGDLRAVIGALLTFVLIVAVLMLIVSAIVWAI
                     AAANGNHVMAGKGRTGVLVALGAAVLAGGGVAWMNWLIALGDQL"
     gene            1496..1768
                     /locus_tag="PLF56_10045"
     CDS             1496..1768
                     /locus_tag="PLF56_10045"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_008357457.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="DUF6112 family protein"
                     /protein_id="HOQ53940.1"
                     /translation="MDINIDPNSDGLPGIAQLRTIVGAVMTVGLILSVLALIISAIVW
                     GFGANSSNPHLAGRGKIGVLVSCGAAVICGASVTLINFFWNVGQQV"
     gene            1810..3228
                     /locus_tag="PLF56_10050"
     CDS             1810..3228
                     /locus_tag="PLF56_10050"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_013601307.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="conjugal transfer protein TrbL"
                     /protein_id="HOQ53941.1"
                     /translation="MGVCDIPLVSTVCDTAGEAAASLVSAPFDWLAGAMGAAAGWLFE
                     AVWSVFDTTTLVDVTSDEYVAVYNILFGIAIFVMLIFFCLQLITGLIRRDPTALSRAA
                     LGLAKSVLGSFVVITLTALLLEIVDQLCVGIIQAAGETTESMGDKIALLAAGLVGINI
                     AAPGVGAIITIFLAGLAIAAAAIVWLSLLVRKALLLVAIVFAPLAFSGASWDASRGWI
                     SKWTMFVIALICSKLVLVVMFLVAITQVSAPIDGDLASVADPIAGIVLMAMAAFAPYL
                     TYKFIAFVGFDMYHAIGSEQDAKNALNRPVPTPSKPQGGAEPKKVLDGDNDGGGPGGG
                     GKKPPEPKNPAPASSGTAGAGGKTAAGSSAGAGAGGGAGAGAGARAGAAAGPAAAAVV
                     AAKVAKDAATAGPKAGKALGNQGDSAADSAGQTDTTPPPAAQAPPPSTPAPKTPSAGP
                     SAPGSRPPKQPPPPAPKPTGKE"
     gene            3234..>3692
                     /locus_tag="PLF56_10055"
     CDS             3234..>3692
                     /locus_tag="PLF56_10055"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_017883883.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="PrgI family protein"
                     /protein_id="HOQ53942.1"
                     /translation="MSAKQNEPAASSGELVPVKFSRLTRRGILLGLSLSQLITLAIGI
                     LSIVGALYAGGGLLLAYTAPVWVIAACLTWIPVAGRPAVEWLPVAVWWLWRTTGGQLL
                     YRRRVVKPRPVGTLALPGDQARLREYTDPETEAGMIHDPTAGTLTVVCEVT"
BASE COUNT          550 a         1396 c         1188 g          558 t
ORIGIN      
        1 ggtcagacgg tcgcgaccgc gtatgcgcac atgtgggagc acggcatcca cgtccaagcc
       61 ggcgacaccg tgaccgccgg gcagcacatc ggcgacatcg gctcctccgg caactcgacc
      121 ggcccgcacc tgcatttcga ggtcagcctc ggcggcaccg atggcgagca caccgacccc
      181 gccgcctggc tcaacagcca tgacgtagcc gacctgcccg aacccgagac cggcacaccg
      241 ggccaggagt gcgaccccga caccggcggt gcgcctggtg gggcgccgga gccgttcgag
      301 ggcgacccga accagacggt cgacgacccc accagcggcg ggcagatcac cgcccgcctg
      361 ctgcacctgt acacccagac catcgccgcg ttccccgaca cgagctgggt ctgctactca
      421 ccccggcccg gcaccgtctc cgaacacccc ctcggccggg cctgcgacgg caccttcggc
      481 aacgccatcg gctcccagcc caccccggca cagcgcgagc tcgggtggga ggtcaccaac
      541 tggatgaagg accacgccga agctctgggc gtggagtacc tgatctggga cggccgcatc
      601 tggtccatat cccgcgacgc cgaaggctgg cgcccctacg acggcggcgg gatgcatgac
      661 cccggcgacg tcaccggcgg ccactacgac cacctgcacg tcaccgtgtc tcagaatccg
      721 tgattgttcg cttacctctg atgcacgaat cttgcgtcag aggtaagcga cgggtcggcc
      781 accgcccggg tcaccgcggc ccgctgagac ggcgcgggcg atccgagcga gtgccctggt
      841 cgcggcgttc cgcaggacca ggcgggcggt cccggcgcag ttgggccagc gagtcgcacc
      901 taccggcatg agggctattc cggcagttcg atcatcggac accaccagca tcgcgtctac
      961 ccgctgcacc cgcgaagggc acgccagtgg tgatgcggca tgaacgtgtt ccccgacttc
     1021 gacgggctcg gcggcatcgg cgacctgcgg gccgtgatcg gcgcgctgct gacgttcgtg
     1081 ctgatcgtcg cggtgctgat gctgatcgtc tccgcgatcg tgtgggccat cgccgccgcg
     1141 aacggcaacc acgtcatggc cggcaagggc cgcaccggcg tcctcgtcgc cctcggcgcc
     1201 gccgtgctgg ccggcggcgg agtggcatgg atgaactggc ttatcgccct cggcgaccaa
     1261 ctctgacacc cgccctgcgc aaccatcgcc accgatgccg tctcaccgtg ccccgggtgc
     1321 gacatccgtc tgtcgcgggc ctctcggcgg gcgggcgcgg tgcgtggacg tgtgcgcgca
     1381 cctgatgcgt gaaccccgtc cgtgttcccc gccttcgcgg cgggtccgca tccgtcagga
     1441 gcccatcgtg ttcgacctcg ccaccgccct tctcgccgca cccgtcgtgg tgccgatgga
     1501 catcaacatc gaccccaaca gtgacggcct gcccggcatc gcgcagttgc gcaccatcgt
     1561 cggcgccgtc atgacggtcg gcctcatcct ctccgtcctc gccctcatca tcagcgcgat
     1621 cgtgtggggc ttcggcgcca acagctccaa cccccacctc gcgggcagag gcaagatcgg
     1681 cgtcctcgtg agctgcggcg ccgccgtcat ctgcggcgcc tcggtgacgc tcatcaactt
     1741 cttctggaac gtcggccagc aggtctgacc cacccaccaa acgtctcaac ttctgaggag
     1801 tgatctgcgg tgggtgtatg cgatatcccc ctggtctcga ccgtctgcga caccgccggc
     1861 gaagccgcag ccagcctggt ctccgccccg ttcgactggc tcgccggcgc gatgggcgcc
     1921 gccgccggct ggctcttcga ggccgtctgg tcggtgttcg acaccaccac cctcgtggat
     1981 gtcaccagcg atgagtacgt ggcggtctac aacatcctgt tcggcatcgc gatcttcgtg
     2041 atgctgatct tcttctgcct ccaactcatc accggactca tcagacgcga ccccaccgca
     2101 ctgtcgagag cggcccttgg gctcgccaag agcgtgctcg ggtcgttcgt ggtcatcacc
     2161 ctgaccgccc tgctgctgga gatcgtggac cagctctgcg tcgggatcat ccaggccgcc
     2221 ggggagacca ccgagagcat gggcgacaag atcgccctgc tcgccgccgg cctggtcgga
     2281 atcaacatcg ccgcaccagg agtcggcgcg atcatcacca tcttcctcgc cggcctcgcc
     2341 atcgccgctg ccgcgatcgt ctggctctca ctactggtgc gcaaggcgct gctgctggtg
     2401 gcgatcgtgt tcgcccccct cgccttctcc ggggcctcgt gggacgccag ccgggggtgg
     2461 atcagcaagt ggacgatgtt cgtcatcgcc ctgatctgct ccaagctcgt cctggtcgtg
     2521 atgttcctcg tcgccatcac ccaggtcagc gcgccgatcg atggggacct ggcctcggtg
     2581 gccgacccga tcgcggggat cgtgctgatg gcgatggccg cgttcgcgcc ctacctgacg
     2641 tataagttca tcgcgttcgt cgggttcgac atgtaccacg ccatcggctc cgaacaggac
     2701 gcgaagaacg ccctcaaccg acccgtcccc accccctcga agccgcaggg cggcgcggag
     2761 ccgaagaagg tcctcgacgg tgacaacgac ggcggcggtc ctggaggtgg cgggaagaag
     2821 ccgccggagc cgaagaaccc cgcacccgcc tccagcggca cggctggcgc aggcggcaag
     2881 accgccgccg gcagctccgc aggagccggt gccggtggcg gggccggagc gggcgctggg
     2941 gctagggcgg gtgcggcggc tggcccagcc gccgccgcgg tggtggccgc gaaagtcgcc
     3001 aaagacgccg ccaccgccgg tcccaaggcc ggcaaggctc tcggcaatca gggtgacagc
     3061 gccgccgact ccgcgggcca gaccgacacc accccgccac cggccgcgca ggcacccccg
     3121 ccctcgactc cggctcccaa gacgccatcg gcaggcccct cggcgccggg ttcgcgtccg
     3181 ccgaagcagc caccgccccc ggcgccgaag ccgaccggca aggagtagcc gccatgtccg
     3241 cgaagcagaa cgagcccgcc gcgtcgtcgg gcgagctggt gccggtgaag ttctcccgcc
     3301 tcacccgccg cggcatcctc cttggactct cgctctcgca gctcatcacc ctcgccatcg
     3361 gcatcctctc gattgtcggt gccctctacg ccgggggcgg cctcctgctc gcctacaccg
     3421 cccccgtctg ggtgatcgcc gcctgcctga cgtggatacc cgtagcgggg cgtccggcgg
     3481 tggaatggct gccggtcgcg gtctggtggc tctggcgcac caccggcgga cagctcctct
     3541 accggcgccg agtcgtcaag ccccgcccgg tcgggacgct ggcactgccc ggcgaccagg
     3601 cgcggctgcg ggagtacacc gaccccgaaa ccgaggctgg gatgatccac gaccccaccg
     3661 ccggcacgct gaccgtggtc tgcgaggtca cc
//
LOCUS       DAOSUG010000308         3687 bp    DNA     linear   ENV 10-JUN-2024
DEFINITION  MAG TPA_asm: Micropruina sp. isolate AMR_MDS_2195
            NODE_10352_length_3687_cov_4.448789, whole genome shotgun sequence.
ACCESSION   DAOSUG010000308 DAOSUG010000000
VERSION     DAOSUG010000308.1
DBLINK      BioProject: PRJNA925477
            BioSample: SAMN32864262
            Sequence Read Archive: SRR4236673
KEYWORDS    WGS; ENV; Metagenome Assembled Genome; MAG; Third Party Data; TPA;
            TPA:assembly.
SOURCE      Micropruina sp. (wastewater metagenome)
  ORGANISM  Micropruina sp.
            Bacteria; Bacillati; Actinomycetota; Actinomycetes;
            Propionibacteriales; Nocardioidaceae; Micropruina.
REFERENCE   1  (bases 1 to 3687)
  AUTHORS   Abdulkadir,N., Saraiva,J.P., Zhang,J., Stolte,S., Gillor,O.,
            Harms,H. and Rocha,U.
  TITLE     Genome-centric analyses of 165 metagenomes show that mobile genetic
            elements are crucial for the transmission of antimicrobial
            resistance genes to pathogens in activated sludge and wastewater
  JOURNAL   Microbiol Spectr 12 (3), e0291823 (2024)
   PUBMED   38289113
REFERENCE   2  (bases 1 to 3687)
  AUTHORS   Abdulkadir,N.
  TITLE     Direct Submission
  JOURNAL   Submitted (24-MAR-2023) Environmental Microbiology, Helmholtz
            Center for Environmental Research -UFZ, Permoserstr 15, Leipzig
            04318, Germany
COMMENT     The annotation was added by the NCBI Prokaryotic Genome Annotation
            Pipeline (PGAP). Information about PGAP can be found here:
            https://www.ncbi.nlm.nih.gov/genome/annotation_prok/
            
            ##Genome-Assembly-Data-START##
            Assembly Method        :: SPAdes v. 3.11.1
            Genome Representation  :: Full
            Expected Final Version :: Yes
            Genome Coverage        :: Not Applicable
            Sequencing Technology  :: Illumina MiSeq
            ##Genome-Assembly-Data-END##
            
            ##Genome-Annotation-Data-START##
            Annotation Provider               :: NCBI
            Annotation Date                   :: 05/04/2023 04:57:10
            Annotation Pipeline               :: NCBI Prokaryotic Genome
                                                 Annotation Pipeline (PGAP)
            Annotation Method                 :: Best-placed reference protein
                                                 set; GeneMarkS-2+
            Annotation Software revision      :: 6.5
            Features Annotated                :: Gene; CDS; rRNA; tRNA; ncRNA
            Genes (total)                     :: 2,776
            CDSs (total)                      :: 2,750
            Genes (coding)                    :: 2,714
            CDSs (with protein)               :: 2,714
            Genes (RNA)                       :: 26
            tRNAs                             :: 24
            ncRNAs                            :: 2
            Pseudo Genes (total)              :: 36
            CDSs (without protein)            :: 36
            Pseudo Genes (ambiguous residues) :: 0 of 36
            Pseudo Genes (frameshifted)       :: 2 of 36
            Pseudo Genes (incomplete)         :: 32 of 36
            Pseudo Genes (internal stop)      :: 2 of 36
            ##Genome-Annotation-Data-END##
FEATURES             Location/Qualifiers
     source          1..3687
                     /organism="Micropruina sp."
                     /mol_type="genomic DNA"
                     /submitter_seqid="NODE_10352_length_3687_cov_4.448789"
                     /isolate="AMR_MDS_2195"
                     /isolation_source="Activated sludge of municipal
                     wastewater treatment plants"
                     /db_xref="taxon:2737536"
                     /environmental_sample
                     /geo_loc_name="Canada"
                     /lat_lon="49.25 N 123.1 W"
                     /collection_date="2015-06-16"
                     /metagenome_source="wastewater metagenome"
                     /note="metagenomic"
     gene            complement(<1..1031)
                     /locus_tag="PLF56_10060"
     CDS             complement(<1..1031)
                     /locus_tag="PLF56_10060"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_013865663.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="AAA family ATPase"
                     /protein_id="HOQ53943.1"
                     /translation="MARTAVSHRCSECGWTSARWVGRCNQCQAWGSMSPHAPAASANT
                     GRSVPTSVARPIGDVPLHEAASTSTGVGELDRVLGGGIVAGAVVLLAGEPGVGKSTLL
                     LDVAARWAAGGRRTLYVSAEESAAQVRLRADRTGALHDQLFLAAETDLGAVLAHIEQL
                     DPSLLVLDSVQAVTVTGADGVTGGVAQVREVTNALVRVAKQRRLPVLLVGHVTKDGTV
                     AGPRLLEHLVDVVLSFEGDRHSGLRLVRASKNRFGPADEVGCFEMTERGIHEVADPSH
                     LFTSARAEPVPGTALTVTQEGRRPLLSEVQALVAPSAAQTPRRVSSGLDSGRVSVILA
                     VLERKARVRV"
     gene            complement(1478..2116)
                     /locus_tag="PLF56_10065"
     CDS             complement(1478..2116)
                     /locus_tag="PLF56_10065"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_002544240.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="VOC family protein"
                     /protein_id="HOQ53944.1"
                     /translation="MHVDHLTFAAGPDGLKAEVERLGDLLGAKFRDGGFHPRFGTRNN
                     ILPLADDRYLEVVEVLEHPAAEKAPFGQAVRARSEMGGGWLGWVVSVPDLAPYEVRLG
                     RESVRGSRHFPDGRLLEWQQIGVKGLMSDPQLPYFIRWTSDESVLPRALPGGDLQLTE
                     LEIAGNEERVEDWIGAEVDGVFDGVNIRFTSPNGQPGIDAATFNTPHGLVRI"
     gene            2184..3002
                     /locus_tag="PLF56_10070"
     CDS             2184..3002
                     /locus_tag="PLF56_10070"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_016669159.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_function: GO:0016853 - isomerase activity [Evidence
                     IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="sugar phosphate isomerase/epimerase"
                     /protein_id="HOQ53945.1"
                     /translation="MAGVQGSSSGVKVALSTSSVYPETTAAGFEVAGLLGYDGVELMV
                     GIDPLAADVDAVQRLRDYHQIPVLAVHAPCLLITQRVWGTDPWAKLERAALAATQLGA
                     DVVVVHPPFRWQRSYASGFVQGVRELVQKTGVTIAVENMFPWRAPGGGDFKAYKPSWD
                     PSDLDYDHLTLDLSHAATARQNSLELVRRWGDRLAHVHLTDGNGSAKDEHLLPGQGNQ
                     QADEVLAELARRGYAGHVVLEVNTNDLDRAGREAALAQALAFARTHLAAASEPR"
     gene            2999..>3687
                     /locus_tag="PLF56_10075"
     CDS             2999..>3687
                     /locus_tag="PLF56_10075"
                     /EC_number="3.4.-.-"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_014845559.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_component: GO:0016020 - membrane [Evidence IEA];
                     GO_function: GO:0004222 - metalloendopeptidase activity
                     [Evidence IEA];
                     GO_process: GO:0071586 - CAAX-box protein processing
                     [Evidence IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="CPBP family intramembrane metalloprotease"
                     /protein_id="HOQ53946.1"
                     /translation="MSIVPDAAQPALRRPGLEVLLVLGVSLGQSAVYSILSILEKLSR
                     PEPLGQQTTTINTSVTPDRPWLDLSYQLAAIVFPLVPALLALYLLRLTNDRERIGFDL
                     TQSWRDLGRGLGLAAAIGIPGLALYFGARAVGLNTNVQPANLAENWWTVPVLVGLAVM
                     NGVLEEVVIVGFWFTRMAQQGWSIAAVLIGSAVIRGAYHLYQGFGGFVGNLVMGLVFG
                     ALYLRWKRVGPL"
BASE COUNT          531 a         1232 c         1336 g          588 t
ORIGIN      
        1 acgcgcaccc gggccttgcg ttcgagaacc gccaggatca ccgagactcg gccgctgtcg
       61 agcccggaac tgacccgcct gggcgtctgc gcagcggacg gcgcgaccag ggcctgcacc
      121 tcgctgagca gcggacgcct gccctcttgg gtgacggtga gtgcggttcc cggcaccggc
      181 tcggcgcggg ccgaggtgaa caggtggctg gggtctgcca cctcgtggat gccgcgctcg
      241 gtcatttcga agcagcccac ctcgtcggcc gggccgaatc ggttcttgct ggcccggacg
      301 agccgtaggc cgctgtgccg gtcgccctcg aacgacagca cgacgtcgac caggtgctcc
      361 agcagccgtg ggccggccac cgttccgtct ttggtgacgt ggcccaccag tagcaccggc
      421 agcctgcgct gtttcgccac tcgcaccaat gcgttggtca cctcgcgaac ctgcgccacc
      481 ccacccgtga cgccgtcggc cccggtgacc gtgaccgcct gcaccgagtc gagcaccagc
      541 aggctggggt cgagctgttc gatgtgcgcc agcaccgcac ccaggtcggt ttcggctgcc
      601 aggaagagct ggtcgtgcag tgcgccggtg cggtcggccc gcagccgcac ctgcgccgcc
      661 gactcttcgg ccgacacata cagggtgcgc cggccaccgg ccgcccagcg tgccgcgacg
      721 tcgagcagca gggtggactt gccgactcct ggttcgccgg cgagcagcac cacagcgccg
      781 gccacgatgc cgccgccgag tacccggtcg agttcgccga cgccggtcga ggtggacgcg
      841 gcctcgtgca gcggcacgtc accgatcgga cgcgccacgc tggtgggtac cgagcgcccc
      901 gtgttcgccg acgcggcggg tgcgtggggc gacatggagc cccacgcctg gcattggttg
      961 cagcgcccca cccagcgggc gctggtccag ccgcactcgg aacagcggtg actgacagcg
     1021 gttctggcca tgcaccgaag gctaggcgtc gcctccgaca cttttcggga cgcggctccg
     1081 cgtgagcgtg ctcggtcttc cagaggggct gccggcagcc aatcgcgggg tgctcgtcgt
     1141 ctcgggcttg ctccgggatg tcggcgcggg cagagatccc ggcgttcgcc gggatgacgg
     1201 ggtcgccacg gtgagggaag ggctgatcaa ggcgcggggg tactcgtcgt ctcgggcttg
     1261 ctccgggatg tcggcgcggg cagagatccc ggccgtgccg ggatgacggg ggtggcgacg
     1321 tgtgcggagg cgctgatcag tgtgcggggt actcgtcgtc ccggggttgt gccgggatgt
     1381 cggcgcaggc agatcccggc gtgcgccggg atgacggggg tggcgacgtg taaggaagcg
     1441 ctgatcagtg cgccgggatg acgggggtca cgacgcccta gatgcgtacc agtccgtgcg
     1501 gggtgttgaa ggtggcggcg tcgatgccgg gctgaccgtt gggtgaggtg aagcgaatgt
     1561 tgacgccgtc gaagactccg tcgacctcgg cgccgatcca gtcctcgacc cgctcctcgt
     1621 tgccggcgat ctcgagctcg gtcagttgca ggtcaccacc aggcagggcc cgcggtagta
     1681 ccgactcgtc gctggtccac cgaatgaagt agggcagctg cgggtcggac atcaggccct
     1741 tgacgccgat ctgctgccat tcgagcagcc gaccgtccgg aaagtgccgt gacccacgga
     1801 cggactcgcg gcccagcctg acctcgtagg gggccaggtc gggcaccgag accacccacc
     1861 cgagccagcc gccgcccatt tcggatcggg cccggaccgc ctgcccgaag ggcgcctttt
     1921 cggcggcggg gtgctcgagc acctcaacca cctcgagata gcggtcatcg gccagcggaa
     1981 gaatgttgtt gcgggtgccg aaacggggat ggaatccacc gtcgcgaaac ttggcgccga
     2041 gcagatcgcc caggcgctcc acctctgcct tcaggccgtc agggcccgcc gcgaacgtca
     2101 gatggtcgac atgcatgacc ccattctgtg ggcaggtccg cccggaagcg aaatcgaggg
     2161 gtgaggcggc acagcggcct atcatggcgg gcgtgcaagg gtcctccagc ggcgtgaaag
     2221 tggcgctgtc cacctcgtcg gtttatcccg aaaccacggc cgcgggtttc gaggtggccg
     2281 gcctgctggg ttacgacggc gtggagttga tggtcggcat cgacccactc gcggcagatg
     2341 tcgatgcggt gcaacggctg cgcgactatc accagattcc ggtgctggcc gtgcacgcgc
     2401 cgtgcctgct gatcacgcag cgggtctggg gcaccgatcc gtgggccaag ctcgaacgcg
     2461 ccgccctggc cgccacccag ctgggcgccg acgtggtcgt ggtgcacccc ccgttccgct
     2521 ggcagcgcag ctatgcctcc ggattcgtgc agggggtgcg cgaactggtc cagaagacgg
     2581 gtgtgacgat cgcggtcgag aacatgtttc cctggcgggc acccggtggg ggcgacttca
     2641 aggcgtacaa gccgagctgg gatccgtccg acctcgacta cgaccacctg accttggacc
     2701 tgtcccatgc cgccaccgca cggcaaaact cgctggagct ggtccggcgc tggggcgacc
     2761 ggttggcgca cgtccatctg accgacggca acggctcggc caaggacgag cacctgctgc
     2821 ccgggcaggg taatcagcag gccgacgagg tgctcgccga gttggcccgc cgaggctatg
     2881 ccggtcacgt ggtgctcgag gtgaacacca acgatctcga ccgagccggc cgggaggcgg
     2941 cgctggcgca ggcgctggcg ttcgcgcgga cgcacttggc cgcggccagc gaaccgcggt
     3001 gagcatcgtc ccggacgccg cgcagcccgc gttgcgacgg cccggcctgg aggtgctgct
     3061 ggtgctgggc gtatcgctcg gccagtcggc ggtctactcc atcctgtcga tcctcgaaaa
     3121 gctcagccgt cccgaaccac tggggcagca gaccaccacg atcaacacct cggtgacgcc
     3181 cgatcgtccg tggctcgacc tcagctatca gctggccgcg atcgtgtttc cgctcgtccc
     3241 ggccctgttg gcgctgtacc tgctgcggct gaccaacgat cgtgaacgta tcgggttcga
     3301 cctcacccag tcctggcgcg acctgggtcg cgggctcggg ctggccgccg cgatcggcat
     3361 tccggggctg gcgctgtact tcggggcgcg cgcggtgggg ctgaacacca acgtgcagcc
     3421 ggccaacctg gccgagaact ggtggacggt gccggtgctg gtcgggctgg cggtgatgaa
     3481 cggcgtgctc gaagaggtgg tgatcgtcgg gttctggttc acccgcatgg cgcagcaggg
     3541 ctggtcgatc gccgcggtgc tgatcggcag cgcggtgatc cggggcgcct accacctcta
     3601 tcagggcttc ggcgggttcg tcggcaacct ggtgatgggc ctggtgttcg gcgcgctgta
     3661 cctgcgctgg aagcgggtcg ggccgct
//
LOCUS       DAOSUG010000309         3681 bp    DNA     linear   ENV 10-JUN-2024
DEFINITION  MAG TPA_asm: Micropruina sp. isolate AMR_MDS_2195
            NODE_10369_length_3681_cov_4.172918, whole genome shotgun sequence.
ACCESSION   DAOSUG010000309 DAOSUG010000000
VERSION     DAOSUG010000309.1
DBLINK      BioProject: PRJNA925477
            BioSample: SAMN32864262
            Sequence Read Archive: SRR4236673
KEYWORDS    WGS; ENV; Metagenome Assembled Genome; MAG; Third Party Data; TPA;
            TPA:assembly.
SOURCE      Micropruina sp. (wastewater metagenome)
  ORGANISM  Micropruina sp.
            Bacteria; Bacillati; Actinomycetota; Actinomycetes;
            Propionibacteriales; Nocardioidaceae; Micropruina.
REFERENCE   1  (bases 1 to 3681)
  AUTHORS   Abdulkadir,N., Saraiva,J.P., Zhang,J., Stolte,S., Gillor,O.,
            Harms,H. and Rocha,U.
  TITLE     Genome-centric analyses of 165 metagenomes show that mobile genetic
            elements are crucial for the transmission of antimicrobial
            resistance genes to pathogens in activated sludge and wastewater
  JOURNAL   Microbiol Spectr 12 (3), e0291823 (2024)
   PUBMED   38289113
REFERENCE   2  (bases 1 to 3681)
  AUTHORS   Abdulkadir,N.
  TITLE     Direct Submission
  JOURNAL   Submitted (24-MAR-2023) Environmental Microbiology, Helmholtz
            Center for Environmental Research -UFZ, Permoserstr 15, Leipzig
            04318, Germany
COMMENT     The annotation was added by the NCBI Prokaryotic Genome Annotation
            Pipeline (PGAP). Information about PGAP can be found here:
            https://www.ncbi.nlm.nih.gov/genome/annotation_prok/
            
            ##Genome-Assembly-Data-START##
            Assembly Method        :: SPAdes v. 3.11.1
            Genome Representation  :: Full
            Expected Final Version :: Yes
            Genome Coverage        :: Not Applicable
            Sequencing Technology  :: Illumina MiSeq
            ##Genome-Assembly-Data-END##
            
            ##Genome-Annotation-Data-START##
            Annotation Provider               :: NCBI
            Annotation Date                   :: 05/04/2023 04:57:10
            Annotation Pipeline               :: NCBI Prokaryotic Genome
                                                 Annotation Pipeline (PGAP)
            Annotation Method                 :: Best-placed reference protein
                                                 set; GeneMarkS-2+
            Annotation Software revision      :: 6.5
            Features Annotated                :: Gene; CDS; rRNA; tRNA; ncRNA
            Genes (total)                     :: 2,776
            CDSs (total)                      :: 2,750
            Genes (coding)                    :: 2,714
            CDSs (with protein)               :: 2,714
            Genes (RNA)                       :: 26
            tRNAs                             :: 24
            ncRNAs                            :: 2
            Pseudo Genes (total)              :: 36
            CDSs (without protein)            :: 36
            Pseudo Genes (ambiguous residues) :: 0 of 36
            Pseudo Genes (frameshifted)       :: 2 of 36
            Pseudo Genes (incomplete)         :: 32 of 36
            Pseudo Genes (internal stop)      :: 2 of 36
            ##Genome-Annotation-Data-END##
FEATURES             Location/Qualifiers
     source          1..3681
                     /organism="Micropruina sp."
                     /mol_type="genomic DNA"
                     /submitter_seqid="NODE_10369_length_3681_cov_4.172918"
                     /isolate="AMR_MDS_2195"
                     /isolation_source="Activated sludge of municipal
                     wastewater treatment plants"
                     /db_xref="taxon:2737536"
                     /environmental_sample
                     /geo_loc_name="Canada"
                     /lat_lon="49.25 N 123.1 W"
                     /collection_date="2015-06-16"
                     /metagenome_source="wastewater metagenome"
                     /note="metagenomic"
     gene            <1..240
                     /locus_tag="PLF56_10080"
     CDS             <1..240
                     /locus_tag="PLF56_10080"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_011756618.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="D-beta-D-heptose 1-phosphate
                     adenosyltransferase"
                     /protein_id="HOQ53947.1"
                     /translation="GVTVHHGLSTPDPDEAAVKRAMVRSARRKVVLADSSKVGATLLI
                     SFAALNEIDVLVTDSGLSEADRQQLVNAGLEVVVA"
     gene            237..1196
                     /locus_tag="PLF56_10085"
     CDS             237..1196
                     /locus_tag="PLF56_10085"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_007078324.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="1-phosphofructokinase family hexose kinase"
                     /protein_id="HOQ53948.1"
                     /translation="MIITLTANPSLDRTATLEAPLARGQVQRVTGVTVEPGGKGVNVA
                     RAIHYAGHPVRAVLPSPDSDPILAALRQLGVDYRTVEVAEGVRMNLTLTEADGTTTKL
                     NEPGPLLDDELVEKLAALVVAEATGAEWVVLSGSLPPGVPADFYATLVRRLRPLGVKV
                     AVDTSDEPLLALVDNFPEAAPDLLKPNSDELAQLTGADAEQLEAAAKAGDPSAAVAAS
                     RSLVERGVAAVMGTLGASGAVLVTSEGAWHASPPPIVPRSTVGAGDSSVAGYVLADCG
                     GEAAPDRLRTAVAYGSAAASMAGSALPRPEHLNTAAVVITDLG"
     gene            1226..3277
                     /locus_tag="PLF56_10090"
     CDS             1226..3277
                     /locus_tag="PLF56_10090"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_007077351.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_component: GO:0016020 - membrane [Evidence IEA];
                     GO_function: GO:0022877 -
                     protein-N(PI)-phosphohistidine-fructose phosphotransferase
                     system transporter activity [Evidence IEA];
                     GO_process: GO:0009401 - phosphoenolpyruvate-dependent
                     sugar phosphotransferase system [Evidence IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="fructose-specific PTS transporter subunit EIIC"
                     /protein_id="HOQ53949.1"
                     /translation="MSQDLITADLVVLDVDLGPTKHDVIHNLAELVAKAGRAEASGLE
                     ADALAREEKAPTGLGGGIAIPHCRSGAVTTNSLAFARLKPPVDFGAFDGPADIAFLIA
                     ASEGSDDAHLALLAELARNLMRSEFTDGLRNATTPDEIVTLVNNAVKAEEKTDAAVAA
                     ATPAAAEGKKVMVAVTACPTGIAHTFMAADGLEQAAKAAGVELHVEPQGSGKVTPIDP
                     AIIARASAAIFATDVGVRDKERFAGLPVIESGVKRAINEPAKMIAEAITASTDPNARK
                     VAGDASSAAASAGGSQLSVGKRIQQALMTGVSYMIPFVAAGGLLIALGFAMAGFDVAL
                     APGFHGARDCAQLLWADPAACATAQAAGADGDTIGALIQAAGWNAGFLNYLGALLFTI
                     GGAAFGFLLPALSGYIAYGLAGRPGIAPGFVGGVIAGALSAGFLGALVTGLLAGVIAL
                     WMSNFNVPRWLAGLMPVVIIPLVVTAVVGLIMYLFLGKPLAAITTGLTEGLNGMAASG
                     AGALLGVVLGLMMCFDLGGPVNKAAYLFATAGLAAQTTASFQIMAAVMAAGMVPPLAL
                     ALATTVRKQLFTEVELENGRAAWLLGASFISEGAIPFAAADPLRVIPSMMAGGAVTGA
                     LSMALGVGSRAPHGGLLVVPLMSNAFGFLIALIAGVLVAAAAVIAMKQFWPAKQVAAI
                     A"
     gene            3551..>3681
                     /locus_tag="PLF56_10095"
     CDS             3551..>3681
                     /locus_tag="PLF56_10095"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_015069560.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="CarD family transcriptional regulator"
                     /protein_id="HOQ53950.1"
                     /translation="MTFEVGETVVYPNHGAAVIEDIETRTIKGEDKLYLVLRIVGQN"
BASE COUNT          557 a         1320 c         1197 g          607 t
ORIGIN      
        1 ggcgtcaccg tgcaccacgg tctctccacc cccgatccgg acgaggccgc ggtcaaacgc
       61 gcgatggttc gctccgcgcg tcgcaaggtc gttctggccg attcgtccaa agtgggtgcc
      121 acgctgttga tcagcttcgc cgcactgaat gagatcgatg ttctggtcac cgactcgggc
      181 ttgtccgagg cggatcgtca acagcttgtc aatgcaggat tggaggtagt cgtcgcatga
      241 tcatcaccct caccgcgaat ccgagtctcg accgcacggc cacattggag gccccgctgg
      301 cgcgaggcca ggtgcagcga gtcaccgggg tcaccgtcga gccgggcggc aagggcgtca
      361 acgtcgctcg cgccattcac tacgccggcc acccggtgcg cgcggtgctg ccgtcgccgg
      421 actccgatcc gatcctcgcc gcgctgcgcc aactgggcgt cgactaccgc accgtcgagg
      481 tggccgaggg cgtccgaatg aacctgaccc tgaccgaagc cgacggcacg accaccaagc
      541 tcaacgagcc gggccctctg ctcgacgacg agctggtcga gaaactggcc gcgctggtgg
      601 tggccgaggc caccggagcc gaatgggtgg tgctgtccgg ctcgctgccg ccgggcgtcc
      661 cggccgactt ctacgcaact ctggtgcgcc ggctgcgtcc gctgggcgtg aaagtggccg
      721 tcgacacctc ggacgagccg ctgctcgcgc tggtcgacaa ctttcctgag gcagcccccg
      781 acctgctcaa acccaactcg gacgaactcg cccagctcac cggggccgac gccgaacagc
      841 tcgaggcggc cgcgaaggcc ggcgatccga gcgcggccgt cgcggcgtcc cgcagcctgg
      901 tcgaacgcgg cgtcgccgcc gtgatgggca cgctgggcgc atccggtgcg gtgctggtca
      961 cgtccgaagg cgcttggcac gccagcccgc cgcccatcgt tccgcgcagc accgtcggcg
     1021 cgggcgactc atcggtggcc ggctacgtgc tggccgactg cgggggagaa gccgcccccg
     1081 accgtcttcg aaccgcagtg gcgtacggct ccgccgccgc atccatggcc ggcagtgcgc
     1141 tgccccggcc cgagcacctc aataccgccg ccgtcgtcat caccgacctc ggctgagact
     1201 gcatcaacca cgaaaggatt cacccatgtc gcaggacctg atcacagctg atctggttgt
     1261 gctcgatgtc gatcttgggc cgaccaagca cgacgtgatc cacaacctgg ccgaactggt
     1321 ggccaaggcc ggccgcgccg aagcgtccgg tctcgaagcg gacgccctcg cccgcgaaga
     1381 gaaggccccc accggtctcg gtggcggaat cgccatcccg cattgccgct ccggcgcggt
     1441 gaccaccaac tcgctggcgt tcgcccggct caagccgccg gtcgacttcg gagccttcga
     1501 cggccccgcc gacatcgcct tcctgatcgc ggcctccgag ggttcggacg atgcccacct
     1561 ggctctgctg gccgaactgg cccgcaacct gatgcgctcc gagttcaccg atgggctgcg
     1621 caacgcgacc acgcccgacg agatcgtcac cctggtgaac aacgccgtca aggccgaaga
     1681 gaagaccgac gccgccgttg ctgccgccac cccggctgcc gccgagggca agaaggtcat
     1741 ggtcgcggtc accgcctgcc cgaccggcat cgcgcacacc ttcatggcgg cggacggcct
     1801 cgaacaggcc gcgaaggctg ccggcgtcga actgcacgtg gagccgcagg gctccggcaa
     1861 ggtcaccccg atcgatcccg cgatcatcgc ccgcgcctcg gcggcgatct tcgccaccga
     1921 cgtcggcgtg cgcgacaagg agcggttcgc cggcctgccg gtgatcgaat ccggcgtcaa
     1981 gcgagccatc aacgagcccg ccaagatgat cgccgaggcc atcaccgcct cgaccgatcc
     2041 caacgcccgc aaggtggccg gcgacgcgtc gtccgccgcc gcgtccgccg gtggtagcca
     2101 gctcagcgtc ggcaagcgca tccagcaggc actgatgacc ggcgtgtcct acatgatccc
     2161 cttcgtggcc gccggcggcc tgctgatcgc cctcggcttc gcgatggccg gcttcgacgt
     2221 ggcactcgcg cccggattcc acggggcccg tgactgcgct cagctgctct gggccgaccc
     2281 ggccgcctgt gccaccgcgc aggccgccgg cgccgacggc gacaccatcg gtgcgctgat
     2341 ccaggccgcc gggtggaacg ccggcttcct caactacctc ggcgcactgc tgttcaccat
     2401 cggcggggcg gccttcgggt tcctgttgcc cgcactgtcg ggctacatcg cctacggtct
     2461 cgccggccgt ccgggcatcg cccccgggtt cgtcggtggc gtcatcgcgg gtgcgctgag
     2521 cgccggcttc ctcggtgctc tggtcacggg cctgctggca ggcgtgatcg cgttgtggat
     2581 gtcgaacttc aacgtgccgc ggtggctggc cggcctgatg ccggtggtga tcattccact
     2641 tgtggtcacc gcagtcgtcg gcctgatcat gtacctgttc ctcggcaagc cgctcgcggc
     2701 aatcaccacg ggtctgaccg agggcctcaa cggcatggcg gccagtggcg cgggcgcgct
     2761 gctcggcgtc gtgctcggcc tgatgatgtg cttcgacctc ggtggtccgg tgaacaaggc
     2821 ggcctacctg ttcgccaccg ccggactcgc cgcgcagacc accgcatcgt tccagatcat
     2881 ggccgctgtg atggccgccg gcatggtgcc gcccctggcg ctggccctgg ccaccaccgt
     2941 ccgcaagcag ctgttcaccg aggtcgagct cgagaacggc cgcgcagcct ggctgctcgg
     3001 tgcgtccttc atctccgagg gtgccattcc gttcgctgcg gctgatccgc tgcgggtgat
     3061 tccctcgatg atggccggtg gcgcggtgac gggtgccctg tcgatggccc tcggcgtcgg
     3121 ttcgcgggcc ccgcacggcg gcctgctggt cgtgccgctg atgagcaacg cgttcgggtt
     3181 cctgatcgca ctgatcgccg gcgtactggt cgcagccgcc gcagtgatcg ccatgaagca
     3241 gttctggccc gccaagcagg tcgcggccat cgcctgaccc gtacgcaacc gagcccgccg
     3301 gacgtcatcg tccggcgggc tcgttgtgcg ttcgcgcgcg gtgaacaccc gtctatccca
     3361 catcatcgag caggcacatt tggtgttgtc agagccatct ttacggttcg cccgacacga
     3421 tcgggcaatg cctccggcat acctgtcgaa cctttgtcaa ccccctaaag tgcctctggc
     3481 taggacgaac gtttcgatta gggagccctt acgtgctacc atgggtgact gggaaagggg
     3541 tctgcacaag atgacttttg aagtcggtga gacggtggtc tacccaaatc atggggcggc
     3601 cgtcatcgaa gacatagaaa cccgtacgat caaaggcgag gacaagctct atctcgttct
     3661 gcgcattgtc ggccagaacg a
//
LOCUS       DAOSUG010000310         3660 bp    DNA     linear   ENV 10-JUN-2024
DEFINITION  MAG TPA_asm: Micropruina sp. isolate AMR_MDS_2195
            NODE_10437_length_3660_cov_3.405270, whole genome shotgun sequence.
ACCESSION   DAOSUG010000310 DAOSUG010000000
VERSION     DAOSUG010000310.1
DBLINK      BioProject: PRJNA925477
            BioSample: SAMN32864262
            Sequence Read Archive: SRR4236673
KEYWORDS    WGS; ENV; Metagenome Assembled Genome; MAG; Third Party Data; TPA;
            TPA:assembly.
SOURCE      Micropruina sp. (wastewater metagenome)
  ORGANISM  Micropruina sp.
            Bacteria; Bacillati; Actinomycetota; Actinomycetes;
            Propionibacteriales; Nocardioidaceae; Micropruina.
REFERENCE   1  (bases 1 to 3660)
  AUTHORS   Abdulkadir,N., Saraiva,J.P., Zhang,J., Stolte,S., Gillor,O.,
            Harms,H. and Rocha,U.
  TITLE     Genome-centric analyses of 165 metagenomes show that mobile genetic
            elements are crucial for the transmission of antimicrobial
            resistance genes to pathogens in activated sludge and wastewater
  JOURNAL   Microbiol Spectr 12 (3), e0291823 (2024)
   PUBMED   38289113
REFERENCE   2  (bases 1 to 3660)
  AUTHORS   Abdulkadir,N.
  TITLE     Direct Submission
  JOURNAL   Submitted (24-MAR-2023) Environmental Microbiology, Helmholtz
            Center for Environmental Research -UFZ, Permoserstr 15, Leipzig
            04318, Germany
COMMENT     The annotation was added by the NCBI Prokaryotic Genome Annotation
            Pipeline (PGAP). Information about PGAP can be found here:
            https://www.ncbi.nlm.nih.gov/genome/annotation_prok/
            
            ##Genome-Assembly-Data-START##
            Assembly Method        :: SPAdes v. 3.11.1
            Genome Representation  :: Full
            Expected Final Version :: Yes
            Genome Coverage        :: Not Applicable
            Sequencing Technology  :: Illumina MiSeq
            ##Genome-Assembly-Data-END##
            
            ##Genome-Annotation-Data-START##
            Annotation Provider               :: NCBI
            Annotation Date                   :: 05/04/2023 04:57:10
            Annotation Pipeline               :: NCBI Prokaryotic Genome
                                                 Annotation Pipeline (PGAP)
            Annotation Method                 :: Best-placed reference protein
                                                 set; GeneMarkS-2+
            Annotation Software revision      :: 6.5
            Features Annotated                :: Gene; CDS; rRNA; tRNA; ncRNA
            Genes (total)                     :: 2,776
            CDSs (total)                      :: 2,750
            Genes (coding)                    :: 2,714
            CDSs (with protein)               :: 2,714
            Genes (RNA)                       :: 26
            tRNAs                             :: 24
            ncRNAs                            :: 2
            Pseudo Genes (total)              :: 36
            CDSs (without protein)            :: 36
            Pseudo Genes (ambiguous residues) :: 0 of 36
            Pseudo Genes (frameshifted)       :: 2 of 36
            Pseudo Genes (incomplete)         :: 32 of 36
            Pseudo Genes (internal stop)      :: 2 of 36
            ##Genome-Annotation-Data-END##
FEATURES             Location/Qualifiers
     source          1..3660
                     /organism="Micropruina sp."
                     /mol_type="genomic DNA"
                     /submitter_seqid="NODE_10437_length_3660_cov_3.405270"
                     /isolate="AMR_MDS_2195"
                     /isolation_source="Activated sludge of municipal
                     wastewater treatment plants"
                     /db_xref="taxon:2737536"
                     /environmental_sample
                     /geo_loc_name="Canada"
                     /lat_lon="49.25 N 123.1 W"
                     /collection_date="2015-06-16"
                     /metagenome_source="wastewater metagenome"
                     /note="metagenomic"
     gene            209..1222
                     /locus_tag="PLF56_10100"
     CDS             209..1222
                     /locus_tag="PLF56_10100"
                     /inference="COORDINATES: protein motif:HMM:TIGR01730.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_function: GO:0005215 - transporter activity [Evidence
                     IEA];
                     GO_process: GO:0006810 - transport [Evidence IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="efflux RND transporter periplasmic adaptor
                     subunit"
                     /protein_id="HOQ53951.1"
                     /translation="MIESNDPTDAELKACGEARAQVTLATQNLQTTVQKLLTPSGSKP
                     SGGSTAGGSSGSSGSSGSSGSSKTSSGSSSSSSTATVSAAQVASAKAKLLQAQQELQA
                     AQDDLDNAELIAPISGTVGTVDLTKGGSSSSGSVTIVGTGNAQVSIELPLATRTLVKA
                     GQKVTVASAGSTKILSGTITSISAVETSGTAGDTPTYATVVTVSDPDGLLASGGRASV
                     TIPVASATNAVRVPTSAVTPTGTGTATVSVLESGSETAKSVTVKTGAVGGGWVQITEG
                     ITAGDKVVLADNTAELPTNTSNRRTTSTSTARASASASAAAQPGGASTAQATAQPTAT
                     ATR"
     gene            complement(1311..2216)
                     /gene="hutG"
                     /locus_tag="PLF56_10105"
     CDS             complement(1311..2216)
                     /gene="hutG"
                     /locus_tag="PLF56_10105"
                     /EC_number="3.5.3.8"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_019047576.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_function: GO:0050415 - formimidoylglutamase activity
                     [Evidence IEA];
                     GO_process: GO:0019556 - histidine catabolic process to
                     glutamate and formamide [Evidence IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="formimidoylglutamase"
                     /protein_id="HOQ53952.1"
                     /translation="MIDRAPVPWTGRDDGPGAEHTRWHQMVNGSGPDAPVVIVGFASD
                     EGVRRNQGRVGAAEGPAAIRRMLANLADPGLPVSDLGDVVVADGDLEAGQQRLGVVVG
                     SVLDRSATPVVLGGGHAVAYGSGSGWLDRPETWGVLNLDAHLDLRRAERATSGTPFLQ
                     LAGDLATAGRPFHYSVIGLSRPSNTRALLDEAERLDVSILYDVDTEVSSALAATTALL
                     ERVERVHLTLDLDVLPASVAPGVSAPAAFGVPPAVILRVLRVVAASGKLGLFEVAELN
                     PRFDIDARTARIAARCVDEVVRHLV"
     gene            complement(2213..3391)
                     /gene="hutI"
                     /locus_tag="PLF56_10110"
     CDS             complement(2213..3391)
                     /gene="hutI"
                     /locus_tag="PLF56_10110"
                     /EC_number="3.5.2.7"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_016666387.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_function: GO:0050480 - imidazolonepropionase activity
                     [Evidence IEA];
                     GO_process: GO:0006548 - histidine catabolic process
                     [Evidence IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="imidazolonepropionase"
                     /protein_id="HOQ53953.1"
                     /translation="MSVLIDNISSLVTNDAVHGGVCGVIPDAAVVLDGDRVAWVGPRS
                     ALDGAGADHRLDAGGRAVVPGFVESHSHLVFAGDRAEEFTARMVGRRYEAGGIRSTMA
                     ATRAASDEELDANAARLSAEYLAQGTTTIEIKSGYGQSVHDERRSLEVADRHTWFGTL
                     LAAHVTPPEFAGRTDDYVTMVIDEMIPACAPLATWIDVFCEQGAFDHYQARAVLTAGK
                     AAGLGVKLHANQLTHGPGVKLAVELGATSVDHVCHLDFTDIEALASSDTVATLLPAAD
                     FSTRNPYPDARRLWDAGVTVALGADCNPGTSYTTSMPFVIALAVRELKLTPDEALWAA
                     TAGGARALASDDRGVLRPGAVADLAVLDAPSHVHLAYRPGVPLVAAVVKNGVLASGRL
                     "
     gene            complement(3396..>3660)
                     /locus_tag="PLF56_10115"
     CDS             complement(3396..>3660)
                     /locus_tag="PLF56_10115"
                     /EC_number="4.2.1.49"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_020580340.1"
                     /note="catalyzes the formation of
                     4-imidazolone-5-propanoate from urocanate during histidine
                     metabolism; Derived by automated computational analysis
                     using gene prediction method: Protein Homology."
                     /codon_start=2
                     /transl_table=11
                     /product="urocanate hydratase"
                     /protein_id="HOQ53954.1"
                     /translation="PLLNALVNTASGASWVSIHHGGGVGMGRSIHAGQVCVADGTALA
                     AQKLERVLTNDPGMGVIRHVDAGYEHAAEVARERGVRVPMLEG"
BASE COUNT          581 a         1359 c         1233 g          487 t
ORIGIN      
        1 tgagccgacc gccaccccca gcccgtccac ctcggccacg ccgaccgaga ccgcgacgcc
       61 ctcagagtcc gcgacaccca ccgcgaccgc gactcccacc gaaacggcca cgccgactca
      121 gaccgcaact gcgacggaaa cggcgacacc cacagaggcg gccagtgcca cggcctcagc
      181 ggcggccacg gttaaagacc aggtggacgt gatcgagtcg aacgacccga ccgacgccga
      241 gttgaaggcc tgcggcgagg cgcgtgcgca ggtcacgctg gcgacccaga acctgcagac
      301 gaccgtgcag aagctgctca cgccgagcgg ctcgaagccg tccggtggct cgacagcggg
      361 cggttcgtcg ggttcgagcg gatcgtcggg ttcgagcgga tcgtccaaga cctcgtccgg
      421 ttcgtcgtcg agcagttcga cggcgacggt cagcgcagcg caggtggcct cggccaaggc
      481 gaagctgcta caggcgcagc aggagttgca ggctgcgcag gacgacctcg acaacgccga
      541 gttgatcgct ccgatctcgg gcacggtcgg caccgtcgac ctgaccaagg gcggctcgtc
      601 cagctcgggg tcggtgacca tcgtgggcac cggcaacgcg caggtgagca tcgagttgcc
      661 gctggccacc cgcacgttgg tcaaggccgg ccagaaggtg acggtcgcat ccgccggatc
      721 gaccaagatc ctgagcggca cgatcacctc gatcagcgcc gtcgaaacca gtggcaccgc
      781 aggtgacacc ccgacctacg ccaccgttgt cacggtgtcc gatccggacg ggttgctcgc
      841 ctcgggcggc cgggcgtcgg tgacgattcc ggtggcctct gccaccaacg cggtgcgcgt
      901 gcccacctcg gccgtcaccc cgaccggcac cggcacggcc acggtttcgg tgctcgaatc
      961 cggctccgag acggcgaagt ccgtcacggt gaagaccggc gcggtcggcg gcggctgggt
     1021 gcagatcacc gagggcatca cggccggcga caaggtggtg ctggccgaca acactgccga
     1081 actgcccacc aacaccagca accggcgaac cacctccacc agcacggcgc gagcctcggc
     1141 cagcgcgtcg gcagcggccc aaccgggcgg tgcgtcaacc gcccaggcga ccgctcaacc
     1201 gacggcgaca gccacccgct aggagtttgg ggaactcagc acggttcggg gacgattctc
     1261 agcacggctg ggaatcgtcc ccgaaccacg tccggaccgt cgtcgattcg tcagacgagg
     1321 tgccggacga cctcgtccac acagcgggcg gcgatgcggg ccgtgcgagc gtcgatgtcg
     1381 aagcgcgggt tgagttcggc aacctcgaac aaccccaact tgccggacgc cgcgacaacc
     1441 cgcagcacgc gcaggatcac cgccggcggc actccgaacg cggctggtgc gctgaccccg
     1501 ggggcgaccg aggcgggcag cacgtccaga tcgagggtga ggtgcacccg ctcgacccgc
     1561 tccagcagtg cggttgtcgc ggccagtgcg ctgctcacct cggtgtcgac gtcgtacaga
     1621 atcgatacgt ccagccgttc ggcctcatcg agcagcgcgc gggtgttgct cggtcggctc
     1681 agcccgatca ccgagtaatg gaacggacgc ccggcggtcg cgagatcgcc ggccaactgc
     1741 agaaacggcg tgccggacgt cgcccgctcg gcccgccgca ggtcgaggtg cgcgtccaga
     1801 ttgagcaccc cccacgtctc gggacgatcg agccaccccg aaccgctgcc gtacgccacc
     1861 gcgtgcccgc cgcccagcac caccggcgtg gcgctccgat cgagcaccga cccgacgacc
     1921 actcccagcc gctgctggcc cgcctccagg tcaccgtccg cgaccacgac gtcacccaga
     1981 tcgctcaccg gcaggcccgg atcggccagg ttggccagca tgcggcgtat cgcggccggc
     2041 ccctcggcgg ccccgactcg cccctgatta cgccggacgc cctcgtcgct ggcgaatccg
     2101 acgatcacca ccggggcatc cggaccagag ccgttgacca tctggtgcca acgggtgtgc
     2161 tcggcgcccg gcccgtcgtc ccgccccgtc cacggcaccg gcgcccggtc gatcacagcc
     2221 gcccgctcgc cagcacgccg ttcttgacga cggcggccac cagcggaacc cccggccggt
     2281 aggccagatg cacgtggctg ggggcgtcca gcacggccag gtcggccacc gcaccgggcc
     2341 gcagcacgcc gcggtcgtcg ctggccagcg ccctggctcc cccggcggtg gcggcccaga
     2401 gcgcctcgtc gggcgtcagc ttcaactcgc gaactgccag cgcgatcacg aacggcatcg
     2461 acgtggtgta ggacgtgccc gggttgcagt cggcgcccag cgcgacggtg acgccggcgt
     2521 cccagagccg gcgggcgtcc gggtaggggt tgcgggtgct gaagtcggcc gcgggcagca
     2581 gcgtcgccac cgtgtcggac gacgccagcg cctcgatgtc ggtgaagtcg aggtgacaga
     2641 catgatcgac cgaggtggcc ccgagttcga cggccagctt gaccccaggg ccgtgggtca
     2701 actggttggc gtgcagcttc acccctagtc cggcggcctt tcctgcggtg agaacggccc
     2761 gcgcctgata gtggtcgaag gcgccctgct cacagaagac gtcgatccag gtggcgagcg
     2821 gcgcgcacgc cgggatcatt tcgtcgatca ccatcgtcac gtagtcgtcg gtgcgtccgg
     2881 cgaactcggg cggcgtcacg tgcgcggcca gcagggtgcc gaaccaggtg tgccggtcgg
     2941 ccacctcgag gctgcgccgc tcgtcatgca cgctctggcc gtaccccgat ttgatctcga
     3001 tcgtggtggt gccctgggcg aggtactccg cactcaaccg ggccgcgttg gcgtccagtt
     3061 cctcatcgga cgcggcccgg gtcgcggcca tcgtgctgcg gatgccgccc gcctcgtagc
     3121 gccggcccac catgcgagcg gtgaactcct cggcccgatc gccggcgaac accaaatggc
     3181 tgtggctttc gacaaatccc gggacgacgg cccgtccgcc ggcgtccagc cggtggtcgg
     3241 cgccggcccc gtccagtgcg gagcgcggtc cgacccacgc gacgcggtcg ccgtccagca
     3301 ccaccgccgc gtccgggatc accccgcaca caccgccgtg gactgcgtcg ttggtgacca
     3361 gcgaggagat gttgtcgatc agcacgctca tcgattcagc cttccagcat cggcacccgg
     3421 acgccacgtt cgcgcgccac ctcggcggcg tgctcgtagc cggcgtccac gtgccggatc
     3481 acgcccatgc cgggatcgtt ggtcagcacc cgctccagct tctgcgcggc cagcgcggtg
     3541 ccgtcggcga cacacacctg gccggcgtgg atcgagcggc ccatgcccac gccgccgcca
     3601 tggtggatgc tcacccatga cgccccggac gccgtgttga ccagtgcgtt cagcagcggc
//
LOCUS       DAOSUG010000311         3657 bp    DNA     linear   ENV 10-JUN-2024
DEFINITION  MAG TPA_asm: Micropruina sp. isolate AMR_MDS_2195
            NODE_10448_length_3657_cov_4.436424, whole genome shotgun sequence.
ACCESSION   DAOSUG010000311 DAOSUG010000000
VERSION     DAOSUG010000311.1
DBLINK      BioProject: PRJNA925477
            BioSample: SAMN32864262
            Sequence Read Archive: SRR4236673
KEYWORDS    WGS; ENV; Metagenome Assembled Genome; MAG; Third Party Data; TPA;
            TPA:assembly.
SOURCE      Micropruina sp. (wastewater metagenome)
  ORGANISM  Micropruina sp.
            Bacteria; Bacillati; Actinomycetota; Actinomycetes;
            Propionibacteriales; Nocardioidaceae; Micropruina.
REFERENCE   1  (bases 1 to 3657)
  AUTHORS   Abdulkadir,N., Saraiva,J.P., Zhang,J., Stolte,S., Gillor,O.,
            Harms,H. and Rocha,U.
  TITLE     Genome-centric analyses of 165 metagenomes show that mobile genetic
            elements are crucial for the transmission of antimicrobial
            resistance genes to pathogens in activated sludge and wastewater
  JOURNAL   Microbiol Spectr 12 (3), e0291823 (2024)
   PUBMED   38289113
REFERENCE   2  (bases 1 to 3657)
  AUTHORS   Abdulkadir,N.
  TITLE     Direct Submission
  JOURNAL   Submitted (24-MAR-2023) Environmental Microbiology, Helmholtz
            Center for Environmental Research -UFZ, Permoserstr 15, Leipzig
            04318, Germany
COMMENT     The annotation was added by the NCBI Prokaryotic Genome Annotation
            Pipeline (PGAP). Information about PGAP can be found here:
            https://www.ncbi.nlm.nih.gov/genome/annotation_prok/
            
            ##Genome-Assembly-Data-START##
            Assembly Method        :: SPAdes v. 3.11.1
            Genome Representation  :: Full
            Expected Final Version :: Yes
            Genome Coverage        :: Not Applicable
            Sequencing Technology  :: Illumina MiSeq
            ##Genome-Assembly-Data-END##
            
            ##Genome-Annotation-Data-START##
            Annotation Provider               :: NCBI
            Annotation Date                   :: 05/04/2023 04:57:10
            Annotation Pipeline               :: NCBI Prokaryotic Genome
                                                 Annotation Pipeline (PGAP)
            Annotation Method                 :: Best-placed reference protein
                                                 set; GeneMarkS-2+
            Annotation Software revision      :: 6.5
            Features Annotated                :: Gene; CDS; rRNA; tRNA; ncRNA
            Genes (total)                     :: 2,776
            CDSs (total)                      :: 2,750
            Genes (coding)                    :: 2,714
            CDSs (with protein)               :: 2,714
            Genes (RNA)                       :: 26
            tRNAs                             :: 24
            ncRNAs                            :: 2
            Pseudo Genes (total)              :: 36
            CDSs (without protein)            :: 36
            Pseudo Genes (ambiguous residues) :: 0 of 36
            Pseudo Genes (frameshifted)       :: 2 of 36
            Pseudo Genes (incomplete)         :: 32 of 36
            Pseudo Genes (internal stop)      :: 2 of 36
            ##Genome-Annotation-Data-END##
FEATURES             Location/Qualifiers
     source          1..3657
                     /organism="Micropruina sp."
                     /mol_type="genomic DNA"
                     /submitter_seqid="NODE_10448_length_3657_cov_4.436424"
                     /isolate="AMR_MDS_2195"
                     /isolation_source="Activated sludge of municipal
                     wastewater treatment plants"
                     /db_xref="taxon:2737536"
                     /environmental_sample
                     /geo_loc_name="Canada"
                     /lat_lon="49.25 N 123.1 W"
                     /collection_date="2015-06-16"
                     /metagenome_source="wastewater metagenome"
                     /note="metagenomic"
     gene            complement(<1..412)
                     /locus_tag="PLF56_10120"
     CDS             complement(<1..412)
                     /locus_tag="PLF56_10120"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_009881988.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="hypothetical protein"
                     /protein_id="HOQ53955.1"
                     /translation="MNEHTPHPEHLDDPDELNELNELDELDELDEDNLVPDDDPLVAE
                     PPHPINWNLLTAADAEVEWFELNRWVRWLRTTYGLPASVIPPFWHHHPELVWELSALH
                     LHWLCAYDPDMSGSAPLGWHRDFADARQRLREWVA"
     gene            complement(427..906)
                     /locus_tag="PLF56_10125"
     CDS             complement(427..906)
                     /locus_tag="PLF56_10125"
                     /inference="COORDINATES: ab initio
                     prediction:GeneMarkS-2+"
                     /note="Derived by automated computational analysis using
                     gene prediction method: GeneMarkS-2+."
                     /codon_start=1
                     /transl_table=11
                     /product="hypothetical protein"
                     /protein_id="HOQ53956.1"
                     /translation="MSINSRPSIRGFIAKDPRELETQDGALYVKVRFGQERKHREEDG
                     SFTDLGKTYDTFVAYDDNVQRIMDNYVPGDRFIAQGYVRKFPKADTGMEGEVFVVTGI
                     GHDTAYTRYDVDRSARRTGPEQTAPEHTTPARTAERDAAAFNPPDRRPASTAPAISM"
     gene            complement(985..2775)
                     /locus_tag="PLF56_10130"
     CDS             complement(985..2775)
                     /locus_tag="PLF56_10130"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_016665860.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="TraM recognition domain-containing protein"
                     /protein_id="HOQ53957.1"
                     /translation="MSSPQGRQTGSFGDELTNAALIALVGMFGVALVLRAAGSVAAFL
                     TGTAQPAAGPASGLGVLFNPGDPAVALDAAAGLNPVVYWIVTAVLLAGLSVGGGWVWV
                     RLRRHTRKTETDPRRLAGTATGHEVSQTASGKALLRRASTLRPSLEAPAPADVGYLLG
                     TSLGKQVWASVEDSILLLGPPRSGKGLHVVINAILDAPGAVVTTSTRPDNLTATLRAR
                     QRDGRPVGVFDPQHLAEGVPAGLRWSPVRGCEDPLTAMVRATGLASATGLSSGGVESG
                     GFWEGKTRTALQALLHAAALDGRPPAELFRWTLDPSIAAEAVAVLTNHPNAATGWADS
                     LGAMIDSDPKTRDSIWQGVSLALSALADPRVLDAVTPRAGEDFDPEAFLTQRGTLYLL
                     ATGAGAGASAALVAAFVEDLVEAARRLAARSPGARLDPPLLLALDEIGNLSPLPSLPT
                     LMAEGGGTGITCLAVLQSLAQAREKWGDNAAGAIWDASIVKIILGGASNSRDLQDLSS
                     LIGERDEYTDSVTLGDHGTRSNQRSIRRVPILPPDRIRTLPFGTGITLLRSAPPIVTD
                     LRAWPTRPDAAQLGKDRSAIEALLRRPAAT"
     gene            complement(2772..3332)
                     /locus_tag="PLF56_10135"
     CDS             complement(2772..3332)
                     /locus_tag="PLF56_10135"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_006594217.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="hypothetical protein"
                     /protein_id="HOQ53958.1"
                     /translation="MSGRNPLHHTSSQQPDETPVVLPHVVIIVTENSTLDVTVDGTPF
                     PPPAAGSEWTRGTFGLLLDAVTKDRTIAVRIEVREVDGTVFTDLIHTRRRPASEADST
                     AAPTRRGRRARQQQPPVLVEVTGDGFVPGEDIAVAVIVAHTDATGTGAARALLDQRQL
                     DALAESPHEVVLYGRISGTTVVRRVP"
     gene            complement(3329..>3657)
                     /locus_tag="PLF56_10140"
     CDS             complement(3329..>3657)
                     /locus_tag="PLF56_10140"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_008359853.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=3
                     /transl_table=11
                     /product="ATP-binding protein"
                     /protein_id="HOQ53959.1"
                     /translation="YGIANMLIFHKLTDLDNVGDQGSAMRSLANSLLANAETRIVYRQ
                     ESDQLGPTAKALGLTGTEQKLLPTLGVGQGLWKIKSRSFISQHQLHPVELDLFRTDAR
                     YLGGQS"
BASE COUNT          486 a         1129 c         1444 g          598 t
ORIGIN      
        1 cggcgaccca ttcacgcagc cgctggcggg cgtcggcgaa gtcgcggtgc cagccgagcg
       61 gggcgctgcc gctcatgtcg gggtcgtagg cgcacagcca gtgcaggtgc agtgcggaca
      121 gttcccagac cagctcgggg tggtggtgcc agaacggtgg gatcacgctg gcgggcagtc
      181 cgtaggtggt gcgcagccat cggacccacc ggttcagctc gaaccattcg acctcggcgt
      241 cggcggcggt gagcaggttc cagttgatcg ggtgcggagg ctcggcaacc agcgggtcgt
      301 cgtccgggac gaggttgtct tcgtccagct cgtccaactc gtccaactcg ttcaactcgt
      361 tcagttcgtc ggggtcgtcc aggtgttcgg ggtggggagt gtgttcgttc atcggtctgc
      421 cgcccctcac atgctgatcg ctggcgcggt gctggctggc cggcggtcgg gcgggttgaa
      481 cgccgctgcg tcccgctcgg cggtgcgtgc cggtgtggtg tgctcggggg cggtctgttc
      541 ggggccggtg cggcgggcgc tgcggtccac gtcgtagcgg gtgtaggcgg tgtcgtgacc
      601 gatgccggtg acgacgaaga cctccccttc catgccggtg tcggccttgg ggaacttgcg
      661 gacatagccc tgggcgatga accggtcgcc ggggacgtag ttgtccatga ttcgctgcac
      721 gttgtcgtcg taggcgacga aggtgtcgta ggtcttgccg aggtcggtga acgagccgtc
      781 ttcctcgcgg tgcttgcgtt cctgcccgaa ccgcaccttc acgtacaggg cgccgtcctg
      841 ggtctccagc tcgcggggat ctttggcgat gaacccccgg atcgagggtc gcgagttgat
      901 cgacatgctg tgctcctgcc cgtgtcaggc cgcgtcaacg gtgcgcggct acaccaggca
      961 ggtgcacgac cggctccccg tgcgtcaggt cgccgccggg cgccgcagca gtgcctcgat
     1021 ggccgagcgg tccttgccga gctgggcggc gtcggggcgg gtgggccagg cgcgcaggtc
     1081 ggtcacgatc ggcggcgcac tacggaggag ggtgatgccg gtgccgaagg gcagggtgcg
     1141 gatgcggtcg ggcggcagga tcggcactct cctgatggag cgttggttcg atctcgtgcc
     1201 gtgatcgccc agggtcacgg agtcggtgta ctcgtcgcgt tcgccgatga gggaggacag
     1261 gtcttggagg tctcgggagt tggaggcgcc gccgaggatg atcttgacga tgctcgcgtc
     1321 ccagatggcg ccggcggcgt tgtcgcccca cttctcccgt gcctgtgcca gtgactgcaa
     1381 gacggcgagg caggtgatac cggtgccgcc gccctcggcc atcagtgtcg gcagcgaggg
     1441 caacggtgag aggttgccga tctcatccag cgcgagcagc agtggtgggt cgagccgggc
     1501 gccgggtgag cgggcggcga ggcggcgggc ggcttccacg aggtcttcca cgaacgccgc
     1561 caccagggcc gcggacgctc cggcccccgc accggtcgcg agcagataca gcgtgccgcg
     1621 ttgggtgagg aaggcttcgg ggtcgaagtc ctcacccgct cgtggggtga cggcgtccag
     1681 gacgcgcggg tcagccaggg cggagagggc gagggagacg ccttgccaga tggagtctcg
     1741 ggtcttgggg tcggagtcga tcatcgcccc gagcgagtcc gcccatccgg tcgctgcgtt
     1801 cgggtggttg gtgaggacgg cgacggcttc ggcggcgatg gaggggtcca gggtccaccg
     1861 gaacagttcg gccggtggcc ggccgtccag ggcggcggcg tgcagcagcg cttggagggc
     1921 ggtgcgtgtc ttgccttccc agaacccgcc ggactccaca ccgccactgc tgagcccggt
     1981 ggctgaggcg aggcccgtgg cgcggaccat cgccgtcagc gggtcctcgc agccgcggac
     2041 cggggaccat cgcagtccgg cggggacgcc ttcggcgagg tgctggggat cgaacacccc
     2101 caccggtcgg ccgtcgcgct ggcgggcgcg gagggtggcg gtgaggttgt ccggtcgcgt
     2161 cgaggtggtg acgacggcac cgggcgcgtc caagatggcg ttgatcacca cgtggagccc
     2221 tttgccggag cggggtgggc cgagcagcag gatcgagtcc tcgaccgatg cccagacctg
     2281 tttgccgagg ctggtgccaa gcaggtagcc cacatccgcc ggtgcgggag cctccaggga
     2341 ggggcggagt gtgctggcgc ggcgcagcag tgccttcccg gaggcggtct ggctgacttc
     2401 gtgcccggtg gcggtgccgg cgaggcggcg ggggtcggtc tcggtcttgc gggtgtggcg
     2461 gcgcagccgc acccacaccc acccgccacc gaccgagagg ccggcgagca ggacggcggt
     2521 gacgatccag tagacgaccg ggttcagccc ggcggcggcg tcgagagcga cggcggggtc
     2581 gccggggttg aacagcactc cgagcccgga cgccggtccc gcggcgggtt gggcggtgcc
     2641 ggtgaggaac gccgccacgc tgccggcggc gcgcaggacg agcgcgacgc cgaacatccc
     2701 gacgagggcg atgagggcgg cgttggtcaa ctcgtccccg aacgagccgg tctgacgacc
     2761 ctgcgggctg ctcacggtac ccgcctgacc acagtggtgc cggagatgcg cccatacagc
     2821 accacctcgt gcgggctctc ggccaaggcg tcgagctggc gttggtcgag gagagcgcgg
     2881 gcggcgccgg tgccggtggc gtcagtgtgg gcgacgatga cggcgacggc gatgtcctcg
     2941 ccgggcacga acccgtcccc ggtgacctca accagcaccg gcggctgctg ctggcgggca
     3001 cgccggccac ggcgcgtcgg tgccgcggtg ctgtctgctt cggaggcggg gcgtcggcgg
     3061 gtgtggatga ggtcggtgaa cacggtcccg tcgacctcac ggacctcgat ccgcaccgcg
     3121 atggtgcggt ccttggtcac cgcgtccagc agcagcccga aggtgccacg tgtccactcc
     3181 gaacccgcgg cagggggcgg gaacggcgtg ccgtcgacgg tgacgtccag ggtgctgttc
     3241 tcggtgacga tgatgacgac gtggggcagc accaccggtg tctcgtcggg ctgctgggac
     3301 gaggtgtggt gcagcgggtt gcggccactc atgactgacc gcccaggtag cgggcatcgg
     3361 tccggaacag gtccagctcg acgggatgga gttggtgttg cgaaatgaaa ctcctggact
     3421 tgatcttcca cagcccctgt ccgaccccga gggtggggag gagcttctgc tcggtaccgg
     3481 tcagcccgag ggccttcgcg gtcgggccga gctggtcgga ctcttgccgg tagacgatcc
     3541 gggtctcggc gttcgcgagc aggctgttgg cgagggagcg catggccgag ccttggtcgc
     3601 ccacgttgtc gaggtcggtg agcttgtgga agatcagcat gttcgcgatc ccgtaat
//
LOCUS       DAOSUG010000312         3631 bp    DNA     linear   ENV 10-JUN-2024
DEFINITION  MAG TPA_asm: Micropruina sp. isolate AMR_MDS_2195
            NODE_10525_length_3631_cov_4.504474, whole genome shotgun sequence.
ACCESSION   DAOSUG010000312 DAOSUG010000000
VERSION     DAOSUG010000312.1
DBLINK      BioProject: PRJNA925477
            BioSample: SAMN32864262
            Sequence Read Archive: SRR4236673
KEYWORDS    WGS; ENV; Metagenome Assembled Genome; MAG; Third Party Data; TPA;
            TPA:assembly.
SOURCE      Micropruina sp. (wastewater metagenome)
  ORGANISM  Micropruina sp.
            Bacteria; Bacillati; Actinomycetota; Actinomycetes;
            Propionibacteriales; Nocardioidaceae; Micropruina.
REFERENCE   1  (bases 1 to 3631)
  AUTHORS   Abdulkadir,N., Saraiva,J.P., Zhang,J., Stolte,S., Gillor,O.,
            Harms,H. and Rocha,U.
  TITLE     Genome-centric analyses of 165 metagenomes show that mobile genetic
            elements are crucial for the transmission of antimicrobial
            resistance genes to pathogens in activated sludge and wastewater
  JOURNAL   Microbiol Spectr 12 (3), e0291823 (2024)
   PUBMED   38289113
REFERENCE   2  (bases 1 to 3631)
  AUTHORS   Abdulkadir,N.
  TITLE     Direct Submission
  JOURNAL   Submitted (24-MAR-2023) Environmental Microbiology, Helmholtz
            Center for Environmental Research -UFZ, Permoserstr 15, Leipzig
            04318, Germany
COMMENT     The annotation was added by the NCBI Prokaryotic Genome Annotation
            Pipeline (PGAP). Information about PGAP can be found here:
            https://www.ncbi.nlm.nih.gov/genome/annotation_prok/
            
            ##Genome-Assembly-Data-START##
            Assembly Method        :: SPAdes v. 3.11.1
            Genome Representation  :: Full
            Expected Final Version :: Yes
            Genome Coverage        :: Not Applicable
            Sequencing Technology  :: Illumina MiSeq
            ##Genome-Assembly-Data-END##
            
            ##Genome-Annotation-Data-START##
            Annotation Provider               :: NCBI
            Annotation Date                   :: 05/04/2023 04:57:10
            Annotation Pipeline               :: NCBI Prokaryotic Genome
                                                 Annotation Pipeline (PGAP)
            Annotation Method                 :: Best-placed reference protein
                                                 set; GeneMarkS-2+
            Annotation Software revision      :: 6.5
            Features Annotated                :: Gene; CDS; rRNA; tRNA; ncRNA
            Genes (total)                     :: 2,776
            CDSs (total)                      :: 2,750
            Genes (coding)                    :: 2,714
            CDSs (with protein)               :: 2,714
            Genes (RNA)                       :: 26
            tRNAs                             :: 24
            ncRNAs                            :: 2
            Pseudo Genes (total)              :: 36
            CDSs (without protein)            :: 36
            Pseudo Genes (ambiguous residues) :: 0 of 36
            Pseudo Genes (frameshifted)       :: 2 of 36
            Pseudo Genes (incomplete)         :: 32 of 36
            Pseudo Genes (internal stop)      :: 2 of 36
            ##Genome-Annotation-Data-END##
FEATURES             Location/Qualifiers
     source          1..3631
                     /organism="Micropruina sp."
                     /mol_type="genomic DNA"
                     /submitter_seqid="NODE_10525_length_3631_cov_4.504474"
                     /isolate="AMR_MDS_2195"
                     /isolation_source="Activated sludge of municipal
                     wastewater treatment plants"
                     /db_xref="taxon:2737536"
                     /environmental_sample
                     /geo_loc_name="Canada"
                     /lat_lon="49.25 N 123.1 W"
                     /collection_date="2015-06-16"
                     /metagenome_source="wastewater metagenome"
                     /note="metagenomic"
     gene            <1..604
                     /locus_tag="PLF56_10145"
     CDS             <1..604
                     /locus_tag="PLF56_10145"
                     /inference="COORDINATES: protein motif:HMM:NF019310.2"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_function: GO:0022857 - transmembrane transporter
                     activity [Evidence IEA];
                     GO_process: GO:0055085 - transmembrane transport [Evidence
                     IEA]"
                     /codon_start=2
                     /transl_table=11
                     /product="MFS transporter"
                     /protein_id="HOQ53960.1"
                     /translation="AQGVSPITAGTLLTVTTVFSVVAAPLIGQFVAYRPFHRSTLVLG
                     TLSFQFAAWTSVLLWPGPAPLWLLVVLVAALGISGPVSVVSFDYARTSNPHDQLGSAT
                     GVINQAGFVATVITVLVIGLILDLLTPAGQPYGREAFGWAMASQAGLWLLGTVQVIRY
                     RGRGRREFRERDAQAYERFRHGDLSVDWNLGVYPSSALDD"
     gene            828..2867
                     /locus_tag="PLF56_10150"
     CDS             828..2867
                     /locus_tag="PLF56_10150"
                     /inference="COORDINATES: ab initio
                     prediction:GeneMarkS-2+"
                     /note="Derived by automated computational analysis using
                     gene prediction method: GeneMarkS-2+."
                     /codon_start=1
                     /transl_table=11
                     /product="hypothetical protein"
                     /protein_id="HOQ53961.1"
                     /translation="MRTLIRPLIAAVTTLLMLLAPLTQATAATNPAPPRSLQTPGIDA
                     HLGYKPQKTCTPSAKPGTTALLKALLKTWGGKSWGISRFCSSGGTSEHKEGRALDWHM
                     DSRKAKDRAKVNEMIRWITANNGEVAYRLGVMYIIWNQKIWSIYYQELGWRKMASRGS
                     WTANHKDHVHTSLSWDGAMAQTSWWTGTVLTVPKLGPCGKASNGSCLPMIGRSSSKTW
                     TKVAVGPFSPYPSTVPNLGGSARVGLTLKAVAGTWMPTGSTIAYQWLRDGKAIAGATG
                     PDYVVAAADLGTALSVRVSATLGGTTVTKTSDSTTDTVKGVLPTPRPSVTGAYAYAST
                     LTGAPGAGFPDGTTFAYQWQRDGKNISGATAPTYTLTAPDVGADVRLRLKASKAGYTT
                     ATTYSVAAEVKPLQFEAAPNPTIEGILRVGGVLTAVPGAWTPSATFGYRWYRNGTQIS
                     GQTKATYTLTSKDLGKAINVRVRGSLAGYAAVNRYSPRTAAVQAGLTVATPTISDTTP
                     RVGQVLSAAPGTWKPAGVAFTYRWYRNGAFITGATSRTYTVTPADHGRTLQVRVEGSK
                     DGYPNASKRSAQSAKVGNGQFKPGTVTVQGTPEVGQTLTADPGTWGSKLNPTLVLTPS
                     FGYQWYAAGKAIKGATARTFAPSSAHRGTKVHVVVTATLSRYTKATAGSAAVSIG"
     gene            complement(3078..>3631)
                     /locus_tag="PLF56_10155"
     CDS             complement(3078..>3631)
                     /locus_tag="PLF56_10155"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_020577433.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_function: GO:0016772 - transferase activity,
                     transferring phosphorus-containing groups [Evidence IEA];
                     GO_process: GO:0016310 - phosphorylation [Evidence IEA]"
                     /codon_start=3
                     /transl_table=11
                     /product="PEP-utilizing enzyme"
                     /protein_id="HOQ53962.1"
                     /translation="ASLAGDLGAFPLFEQRRDTLRRYRALPALPAAIVGRFEPFVWAA
                     DPHRRTDVFVAGAEMATAAPSDEVVTGFAGAIGRVEGSVRRIDLFEDADQLRPGEILV
                     TQLTNIGWTPLFPRAAGIVTDLGAPLSHAAIVARELGVPAVVGCGDATARLHTGDRVL
                     LDGAAGTVRLLARAMEATADPGV"
BASE COUNT          592 a         1326 c         1209 g          504 t
ORIGIN      
        1 ggcccaaggc gtctcgccga tcaccgccgg cactttgctg accgtcacca cggtgttctc
       61 ggtggtggcc gctccgctga tcggccagtt cgtcgcctac cgtccgttcc accgctccac
      121 actcgtgctg ggcacgttga gctttcagtt cgccgcctgg acgtcggtgc tgctgtggcc
      181 cggtccggcg ccgctgtggc tgctggtcgt tctggtggcc gcgctgggca tcagcgggcc
      241 ggtgtcggtg gtcagcttcg actacgcccg gacgtccaac ccgcacgacc agttgggcag
      301 cgccaccggg gtgatcaacc aggccggttt cgtggccacg gtgatcaccg tgctggtgat
      361 cgggctgatc ctcgatctgc tgacccctgc cggccagccg tacggacgtg aggcgttcgg
      421 ctgggcgatg gcgtcgcagg cggggctgtg gctgctcggc accgtgcagg tgattcgtta
      481 ccgcggacga ggccggcgcg agttccgcga gcgcgacgcc caggcctatg aaaggttccg
      541 gcacggcgac ctgagcgtcg actggaacct gggcgtgtac ccctcgtccg cgctggacga
      601 ctgacgccgc aaccctgtgg gccactacgc cggctcgtcc aagacgacga gcccgtacgc
      661 cgtcgccgcc cccctccaca cagcccggtc gccggtcaga acggtgccga tcagcccatg
      721 ggatcctgag aaggctcccg cggaacctga gacagggtgc cgtcccctcg ttctcgactt
      781 accctgctgg cacgatcccg acgccgcgac cgtctgcgag gcagctcatg cgcaccctga
      841 tccgaccgct catcgccgcc gtcaccacgc tgctgatgct gctcgcaccc ctcacccagg
      901 cgacggccgc gacgaacccg gcaccgccgc gctcgctgca aacgccgggc atcgacgccc
      961 acctcggcta caagccgcag aagacctgca ccccgtccgc caaaccgggc accaccgccc
     1021 tgctgaaggc gctgctgaag acctggggcg gcaaatcctg gggcatctcg cggttctgca
     1081 gcagcggcgg aacgtccgaa cacaaggagg gacgggccct cgactggcac atggactcac
     1141 gcaaggccaa ggaccgcgcc aaggtcaacg agatgatcag gtggatcacc gccaacaacg
     1201 gcgaggtggc ataccggctc ggcgtgatgt acatcatctg gaatcagaag atctggtcga
     1261 tctactacca ggaactcggg tggcgaaaga tggccagccg cggctcgtgg acggccaatc
     1321 acaaggacca cgtgcacacc tcgctgtcgt gggacggggc catggcgcag acgtcctggt
     1381 ggaccggcac ggtgctcacc gtgccgaaac ttgggccgtg cggcaaggca tccaacggtt
     1441 cgtgcctgcc gatgatcggg cgctcgtcgt cgaagacctg gaccaaggtg gccgtcggtc
     1501 cgttcagccc ctacccctcg acggtgccca acctgggcgg cagcgcgcgc gtcggattga
     1561 ccctcaaagc ggtcgccgga acgtggatgc cgaccggctc gacgatcgcc taccaatggc
     1621 tgcgggacgg caaggcgatc gcgggcgcga ccggcccgga ctatgtggtg gccgccgccg
     1681 acctgggcac ggcgttgtcg gtgcgggtca gcgccacgct ggggggcacg acggtgacca
     1741 agacctccga ttcgaccacc gacacggtca agggcgtgct gcccacaccg cggccctcgg
     1801 tgacgggcgc gtacgcatac gcgtccaccc tgaccggcgc accaggagcc ggcttcccgg
     1861 acggcacgac gttcgcctac caatggcagc gcgacggcaa gaacatctcg ggcgccacag
     1921 cgcccaccta tacgctcacg gcgccggacg tcggcgcgga cgtccggctg cggctcaagg
     1981 cgtcgaaggc gggctacacc acggccacca cgtactcggt cgccgctgag gtgaagccgc
     2041 tgcagttcga ggcggcaccg aatcccacga tcgagggcat tctgcgggtc ggtggcgtgc
     2101 tgaccgcggt gccgggtgcg tggaccccgt ccgcgacctt cgggtatcgc tggtaccgca
     2161 acggcacgca gatctcgggc cagaccaagg ccacgtacac cctcacgtcc aaggatctgg
     2221 gcaaggcgat caacgtgcgg gtgcgggggt cactggccgg ctacgcggcg gtgaaccgct
     2281 actctccgcg caccgcggcc gtgcaggccg gactcacggt ggccacaccc accatctcgg
     2341 acaccacccc gcgcgtgggt caggtgctga gcgcggcgcc gggcacctgg aagccggcgg
     2401 gggtggcctt cacctaccgc tggtaccgca acggcgcctt catcaccggc gcgaccagcc
     2461 gtacctacac ggtcacgccg gccgaccacg gccgcaccct gcaggtgcgt gtcgaaggat
     2521 cgaaggacgg ctacccgaac gcgtccaagc gctcggcgca gagcgccaag gtgggcaacg
     2581 ggcagttcaa gccgggcacg gtgacggtgc agggcacgcc cgaggtcggc cagaccctga
     2641 ccgcagaccc cggcacctgg ggttcgaagc tgaaccccac actcgtactg accccatcgt
     2701 tcggctacca gtggtacgcg gccggcaagg cgatcaaggg cgcaacggcg cggacgttcg
     2761 cgccgtcgtc cgcgcaccgg ggcacgaagg tgcacgtggt cgtgacggcg acgctgagcc
     2821 ggtacacgaa ggcgacggcc ggctcagcgg cggtgagcat cggctgatca ggtcggtggt
     2881 ttgatcgaac ccgcggtgac gcccgcctcc accaccgtcg ccccggcgca cgccgggatc
     2941 tcccaccaca gacccactga ccaatgaatc agtgcccccg cccaccgtga tccccccacc
     3001 accgtcaccc cggcgcacgc ccggatctcc caccacagac ccactgacgc tcgtcgagat
     3061 ccccgccgat cgagttctca cacacccggg tccgctgtcg cttccatcgc ccgcgccagc
     3121 aatcggacgg tgcccgcagc cccgtcgagc agcacccggt cgccggtgtg cagccgcgcg
     3181 gtggcgtccc cgcagccgac cacggccggc acgccgagct cgcgagccac gatcgcggcg
     3241 tggctgaggg gtgcccccag gtcggtgacg atgccggcgg cccgcggaaa cagcggcgtc
     3301 cacccgatgt tggtcaactg ggtgaccagg atctcgccgg ggcgcaactg gtcggcgtcc
     3361 tcgaacaggt cgatccggcg gacgctgccc tccacccgtc cgatggcgcc cgcaaagccg
     3421 gtcaccactt cgtcggacgg tgccgctgtc gccatctcgg cgccggccac gaagacatca
     3481 gtgcgccggt ggggatcggc ggcccatacg aacggctcga accgccccac gatcgccgcc
     3541 ggcagtgcgg gcagcgcgcg gtagcggcgc agcgtatcgc gtcgctgctc gaacagcggg
     3601 aacgctccga ggtctccggc caagctcgcc a
//
LOCUS       DAOSUG010000313         3628 bp    DNA     linear   ENV 10-JUN-2024
DEFINITION  MAG TPA_asm: Micropruina sp. isolate AMR_MDS_2195
            NODE_10538_length_3628_cov_3.824797, whole genome shotgun sequence.
ACCESSION   DAOSUG010000313 DAOSUG010000000
VERSION     DAOSUG010000313.1
DBLINK      BioProject: PRJNA925477
            BioSample: SAMN32864262
            Sequence Read Archive: SRR4236673
KEYWORDS    WGS; ENV; Metagenome Assembled Genome; MAG; Third Party Data; TPA;
            TPA:assembly.
SOURCE      Micropruina sp. (wastewater metagenome)
  ORGANISM  Micropruina sp.
            Bacteria; Bacillati; Actinomycetota; Actinomycetes;
            Propionibacteriales; Nocardioidaceae; Micropruina.
REFERENCE   1  (bases 1 to 3628)
  AUTHORS   Abdulkadir,N., Saraiva,J.P., Zhang,J., Stolte,S., Gillor,O.,
            Harms,H. and Rocha,U.
  TITLE     Genome-centric analyses of 165 metagenomes show that mobile genetic
            elements are crucial for the transmission of antimicrobial
            resistance genes to pathogens in activated sludge and wastewater
  JOURNAL   Microbiol Spectr 12 (3), e0291823 (2024)
   PUBMED   38289113
REFERENCE   2  (bases 1 to 3628)
  AUTHORS   Abdulkadir,N.
  TITLE     Direct Submission
  JOURNAL   Submitted (24-MAR-2023) Environmental Microbiology, Helmholtz
            Center for Environmental Research -UFZ, Permoserstr 15, Leipzig
            04318, Germany
COMMENT     The annotation was added by the NCBI Prokaryotic Genome Annotation
            Pipeline (PGAP). Information about PGAP can be found here:
            https://www.ncbi.nlm.nih.gov/genome/annotation_prok/
            
            ##Genome-Assembly-Data-START##
            Assembly Method        :: SPAdes v. 3.11.1
            Genome Representation  :: Full
            Expected Final Version :: Yes
            Genome Coverage        :: Not Applicable
            Sequencing Technology  :: Illumina MiSeq
            ##Genome-Assembly-Data-END##
            
            ##Genome-Annotation-Data-START##
            Annotation Provider               :: NCBI
            Annotation Date                   :: 05/04/2023 04:57:10
            Annotation Pipeline               :: NCBI Prokaryotic Genome
                                                 Annotation Pipeline (PGAP)
            Annotation Method                 :: Best-placed reference protein
                                                 set; GeneMarkS-2+
            Annotation Software revision      :: 6.5
            Features Annotated                :: Gene; CDS; rRNA; tRNA; ncRNA
            Genes (total)                     :: 2,776
            CDSs (total)                      :: 2,750
            Genes (coding)                    :: 2,714
            CDSs (with protein)               :: 2,714
            Genes (RNA)                       :: 26
            tRNAs                             :: 24
            ncRNAs                            :: 2
            Pseudo Genes (total)              :: 36
            CDSs (without protein)            :: 36
            Pseudo Genes (ambiguous residues) :: 0 of 36
            Pseudo Genes (frameshifted)       :: 2 of 36
            Pseudo Genes (incomplete)         :: 32 of 36
            Pseudo Genes (internal stop)      :: 2 of 36
            ##Genome-Annotation-Data-END##
FEATURES             Location/Qualifiers
     source          1..3628
                     /organism="Micropruina sp."
                     /mol_type="genomic DNA"
                     /submitter_seqid="NODE_10538_length_3628_cov_3.824797"
                     /isolate="AMR_MDS_2195"
                     /isolation_source="Activated sludge of municipal
                     wastewater treatment plants"
                     /db_xref="taxon:2737536"
                     /environmental_sample
                     /geo_loc_name="Canada"
                     /lat_lon="49.25 N 123.1 W"
                     /collection_date="2015-06-16"
                     /metagenome_source="wastewater metagenome"
                     /note="metagenomic"
     gene            complement(<1..3151)
                     /gene="gltB"
                     /locus_tag="PLF56_10160"
     CDS             complement(<1..3151)
                     /gene="gltB"
                     /locus_tag="PLF56_10160"
                     /EC_number="1.4.1.13"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_008362954.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_function: GO:0003824 - catalytic activity [Evidence
                     IEA];
                     GO_function: GO:0015930 - glutamate synthase activity
                     [Evidence IEA];
                     GO_function: GO:0016491 - oxidoreductase activity
                     [Evidence IEA];
                     GO_process: GO:0006537 - glutamate biosynthetic process
                     [Evidence IEA];
                     GO_process: GO:0006807 - nitrogen compound metabolic
                     process [Evidence IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="glutamate synthase large subunit"
                     /protein_id="HOQ53963.1"
                     /translation="MPTRPAQGLYDPSFEHDACGVAFVAQLSGEASHDIVAKGLTALE
                     NLDHRGATGADAAAGDGAGMLLQVPDAFFRAVADFELPEAGSYAMGMAFLPQSPVKRA
                     QAKRTIEYIAVEEGLDVLGWRAVPTYDGTLSPVSREVMPVFEQLFLSSPNGEQGIALD
                     RLAYPLRQRARNESGVYFASLSARTTVYKGMLTTQQLAEVFPDLTDERIASAMALVHS
                     RFSTNTFPAWELSHPYRLIAHNGEINTVKGNRNWMRAREALLATDLIPGDLERLFPIC
                     TPGGSDSASFDEVLELLHLGGRSLPHAVMMMIPEPWENNAEMDPARKAFYSFHSSLME
                     PWDGPAAVCFTDGTLIGATLDRNGLRPGRYWVSDEGLVVFASEAGVIDIPQSKVIAKG
                     RLQPGRMLLVDMAEHRLIDDDEIKSSLAAEHPYEQWLEEGRVDLNDLPDRQHVVHSAA
                     SVLRRQQVFGYTHEELKIILAPMANSGAEPIGSMGTDTPLAVLSERPRLLFDYFAQLF
                     AQVTNPPLDAIREELVTSLSSTFGPEGNLLEPGPDSCKHIMIGYPVLDSDQLAKLVRI
                     NRDGSMPAYDTHVVRGLYPVEEGAEGLKAKLDELCAEVTASIDRGCRTIILSDRHSNA
                     DLAPIPSLLLTAAVHQHLVRQKRRTEVGLIVEAGDVREVHHVALLVGYGAAAVNPYLA
                     FESAEDLARREWLVKVEPEKAVANVRKALGKGVLKVMSKMGVSTIASYTGAQIFEALG
                     LSTQFVDDYFPGTTSRVEGIGLNEIARTIAARHAMAYPPDRITLPHRTLNVGGEYQWR
                     REGEPHLFDPETVFRLQHSTRTKRYDIFKAYTDRVNDQSKRLMTLRGLLEFSSDRKPV
                     PIDQVEPVSEIVKRFSTGAMSYGSISKEAHETLAVAMNRLGGKSNTGEGGEDADRLHD
                     PVRRSAIKQVASGRFGVTSDYLTNATDIQIKMAQGAKPGEGGQLPGQKVYPWVAKTRH
                     STPGVGLISPPPHHDIYSIEDLKQLIHDLKCANPVARVHVKLVAEVGVGTVAAGVSKA
                     KADVVLISGHDGGTGAAPLT"
BASE COUNT          540 a         1193 c         1267 g          628 t
ORIGIN      
        1 acgtcagcgg cgccgcgccg gtgcccccgt cgtggcccga gatcagcacc acgtccgcct
       61 tggccttcga cacgcccgcg gcgacggttc caacaccgac ctcggccacc agcttgacgt
      121 gcacccgggc gaccgggttc gcgcatttga ggtcgtggat cagctgcttg aggtcttcga
      181 tcgagtagat gtcgtggtgc gggggcgggc tgatcagccc cacgcccggc gtggaatgcc
      241 gggtcttggc cacccaggga tacaccttct ggccgggcag ctggccgccc tcgccgggct
      301 tggcgccctg cgccatcttg atctggatgt cggtggcgtt ggtcaggtag tccgaggtga
      361 ccccgaaccg tccggacgcc acctgtttga tcgccgagcg ccggacgggg tcgtgcaggc
      421 ggtccgcgtc ctcgccgccc tcgccggtgt tcgacttgcc gcccagccgg ttcatggcaa
      481 cggccagcgt ctcgtgcgcc tctttcgaga ttgagccgta gctcatcgcg ccggtggaga
      541 accgcttgac gatctcgctg accggctcga cctggtcgat cggaacgggc ttgcggtcgg
      601 aactgaactc gagcaggccg cgcagcgtca tcagccgctt cgactggtcg ttgacccgat
      661 cggtgtacgc cttgaagatg tcgtagcgct tggtgcgggt cgaatgctgc agccgaaaca
      721 ccgtctcggg atcgaacagg tgcggctcgc cctcgcggcg ccactggtac tcgccgccca
      781 cgttcagcgt gcggtgcggc agggtgatcc ggtccggcgg gtaggccatg gcgtgccggg
      841 cggcgatggt gcgggcgatc tcgttcaggc cgatgccctc gacccggctc gtggtgccgg
      901 gaaagtagtc gtcgacgaac tgggtgctca ggccgagcgc ctcgaagatc tgggcaccgg
      961 tgtacgacgc gatcgtcgag acgcccatct tcgacatcac cttcagcacg cccttaccga
     1021 gcgccttgcg cacgttggcg accgccttct cgggttcgac cttgaccagc cactcgcgcc
     1081 gggccagatc ctcggccgac tcgaaggcca ggtacgggtt gaccgcggcc gcaccgtagc
     1141 cgaccagcag cgccacgtga tgcacctcgc gcacgtcgcc ggcctcgacg atcagcccca
     1201 cctcggtgcg gcgcttctgc cgcaccaggt gctggtgcac cgccgcggtg agcagcaggg
     1261 acggaatcgg tgccaggtcg gcgttgctgt gccggtcgga caggatgatc gtgcgacaac
     1321 cccggtcgat ggacgccgtg acctcggcgc acaactcgtc cagtttggcc ttcagtcctt
     1381 cggcgccctc ttcgaccggg tacaggccgc gcaccacgtg ggtgtcgtag gcgggcatcg
     1441 agccgtcgcg gttgatccgc accagcttgg ccaactggtc cgaatcgagc accggataac
     1501 cgatcatgat gtgcttgcac gagtcggggc ccggttcgag caggttgccc tcgggcccga
     1561 aggtcgagct gagcgaggtg accagctctt cgcgaatggc gtccagcggc gggttggtga
     1621 cctgggcgaa cagttgcgcg aagtagtcga acagcagccg cggacgctcg ctgagcacgg
     1681 ccagcggcgt gtcggtgccc atcgacccga tcggctcggc gccgctgttg gccatcgggg
     1741 caaggatgat cttgagttct tcgtgggtgt agccgaacac ctgctggcgg cgcagcaccg
     1801 aggcggcact gtgcaccacg tgctgacgat cgggcaggtc gttgaggtcg acccggccct
     1861 cttcgagcca ctgctcgtag gggtgctcgg cggccagtga cgacttgatc tcgtcgtcgt
     1921 cgatcagccg gtgctcggcc atgtcgacca gcagcatgcg gcccggctgc agccggccct
     1981 tggcgatcac cttgctctgc ggaatgtcga tcacgcccgc ctcggacgcg aacaccacca
     2041 ggccctcgtc gctgacccag tagcggcccg gacgcagccc gttgcggtcg agggtggctc
     2101 cgatcagggt gccgtcggtg aagcagaccg ccgcgggacc gtcccagggc tccatcagcg
     2161 aggagtggaa cgagtagaac gccttgcggg cgggatccat ctcggcgttg ttctcccagg
     2221 gctccgggat catcatcatc accgcgtgtg gcagggagcg tccgcccagg tgcagcagtt
     2281 cgagcacctc gtcgaagctg gccgagtcgg atccgcccgg ggtgcagatc gggaacaggc
     2341 gttcgaggtc gccggggatc aggtcggtgg cgagcagcgc ctcccgggcg cgcatccagt
     2401 tgcggttgcc cttgaccgtg ttgatctcgc cgttgtgggc gatcagccgg taggggtgcg
     2461 agagctccca ggctggaaag gtgttggtgc tgaaccgtga gtgcaccagg gccatggcgg
     2521 acgcgatgcg ctcgtcggtc aggtcgggaa agacctcggc caactgttgc gtggtgagca
     2581 tgcccttgta gacggtggtg cgggccgaca gcgaggcgaa gtagacgccg gactcgttgc
     2641 gggcgcgctg ccgcagaggg taggcgagtc ggtcgagcgc gatgccctgc tcaccgttcg
     2701 gcgagctcag gaacagctgc tcgaacaccg gcatcacctc gcgcgaaacg gggctcagag
     2761 tgccgtcgta ggtgggcacc gcacgccagc ccagcacgtc caggccctct tcgacggcga
     2821 tgtactcgat ggtgcgcttg gcctgggcac gcttgaccgg cgactgcggc agaaatgcca
     2881 tgcccatcgc gtagctgccg gcttcgggca gttcgaagtc ggcgacggcg cggaagaagg
     2941 cgtccggaac ctgcagcagc atgcccgctc cgtcaccggc cgcggcgtcg gcgccggtgg
     3001 caccgcgatg atcgaggttc tcaagcgcgg tcaggccctt tgccacgatg tcatggctgg
     3061 cttcaccgct gagctgtgcc acgaacgcga caccgcaggc gtcgtgctcg aagctcgggt
     3121 cgtacaaacc ctgcgcgggc ctggtaggca tggacgtcaa agccatgggc gtctctcctc
     3181 atcttccggc gtgggttgcg ggccggaggg tgccgatgcg ctcgccatcc ggcgccaacg
     3241 ctgcacgcgg tgggaagcat acgacacccg tcattgcggg caggttacga cggtttgcat
     3301 ggtggacgag cggtggccgt cacggtgcag ggcccaggag cctggagccc gtggtcgagc
     3361 ctgtcgagcc gctgctttcg tcatccctaa gactcacagc cgggatcttc cttgcgcgcc
     3421 cgccaatgac gcgggagagc ctgttggtcg agcctgttgc accgctgctt tcgccatccc
     3481 ggcgaacgcc gggatctctg ctgcacgcct accaacgacg cgcgagagcc tgtcgagact
     3541 cctgggttgc gtttggttgc cgcggagtgg gggtgcaccc cgtcccgccc cggcaagcct
     3601 tctcggctcc gcaggctccg ccgagaag
//
LOCUS       DAOSUG010000314         3619 bp    DNA     linear   ENV 10-JUN-2024
DEFINITION  MAG TPA_asm: Micropruina sp. isolate AMR_MDS_2195
            NODE_10563_length_3619_cov_3.462682, whole genome shotgun sequence.
ACCESSION   DAOSUG010000314 DAOSUG010000000
VERSION     DAOSUG010000314.1
DBLINK      BioProject: PRJNA925477
            BioSample: SAMN32864262
            Sequence Read Archive: SRR4236673
KEYWORDS    WGS; ENV; Metagenome Assembled Genome; MAG; Third Party Data; TPA;
            TPA:assembly.
SOURCE      Micropruina sp. (wastewater metagenome)
  ORGANISM  Micropruina sp.
            Bacteria; Bacillati; Actinomycetota; Actinomycetes;
            Propionibacteriales; Nocardioidaceae; Micropruina.
REFERENCE   1  (bases 1 to 3619)
  AUTHORS   Abdulkadir,N., Saraiva,J.P., Zhang,J., Stolte,S., Gillor,O.,
            Harms,H. and Rocha,U.
  TITLE     Genome-centric analyses of 165 metagenomes show that mobile genetic
            elements are crucial for the transmission of antimicrobial
            resistance genes to pathogens in activated sludge and wastewater
  JOURNAL   Microbiol Spectr 12 (3), e0291823 (2024)
   PUBMED   38289113
REFERENCE   2  (bases 1 to 3619)
  AUTHORS   Abdulkadir,N.
  TITLE     Direct Submission
  JOURNAL   Submitted (24-MAR-2023) Environmental Microbiology, Helmholtz
            Center for Environmental Research -UFZ, Permoserstr 15, Leipzig
            04318, Germany
COMMENT     The annotation was added by the NCBI Prokaryotic Genome Annotation
            Pipeline (PGAP). Information about PGAP can be found here:
            https://www.ncbi.nlm.nih.gov/genome/annotation_prok/
            
            ##Genome-Assembly-Data-START##
            Assembly Method        :: SPAdes v. 3.11.1
            Genome Representation  :: Full
            Expected Final Version :: Yes
            Genome Coverage        :: Not Applicable
            Sequencing Technology  :: Illumina MiSeq
            ##Genome-Assembly-Data-END##
            
            ##Genome-Annotation-Data-START##
            Annotation Provider               :: NCBI
            Annotation Date                   :: 05/04/2023 04:57:10
            Annotation Pipeline               :: NCBI Prokaryotic Genome
                                                 Annotation Pipeline (PGAP)
            Annotation Method                 :: Best-placed reference protein
                                                 set; GeneMarkS-2+
            Annotation Software revision      :: 6.5
            Features Annotated                :: Gene; CDS; rRNA; tRNA; ncRNA
            Genes (total)                     :: 2,776
            CDSs (total)                      :: 2,750
            Genes (coding)                    :: 2,714
            CDSs (with protein)               :: 2,714
            Genes (RNA)                       :: 26
            tRNAs                             :: 24
            ncRNAs                            :: 2
            Pseudo Genes (total)              :: 36
            CDSs (without protein)            :: 36
            Pseudo Genes (ambiguous residues) :: 0 of 36
            Pseudo Genes (frameshifted)       :: 2 of 36
            Pseudo Genes (incomplete)         :: 32 of 36
            Pseudo Genes (internal stop)      :: 2 of 36
            ##Genome-Annotation-Data-END##
FEATURES             Location/Qualifiers
     source          1..3619
                     /organism="Micropruina sp."
                     /mol_type="genomic DNA"
                     /submitter_seqid="NODE_10563_length_3619_cov_3.462682"
                     /isolate="AMR_MDS_2195"
                     /isolation_source="Activated sludge of municipal
                     wastewater treatment plants"
                     /db_xref="taxon:2737536"
                     /environmental_sample
                     /geo_loc_name="Canada"
                     /lat_lon="49.25 N 123.1 W"
                     /collection_date="2015-06-16"
                     /metagenome_source="wastewater metagenome"
                     /note="metagenomic"
     gene            <1..566
                     /locus_tag="PLF56_10165"
     CDS             <1..566
                     /locus_tag="PLF56_10165"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_008063311.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_component: GO:0016020 - membrane [Evidence IEA];
                     GO_function: GO:0005215 - transporter activity [Evidence
                     IEA];
                     GO_function: GO:0005524 - ATP binding [Evidence IEA];
                     GO_function: GO:0016887 - ATP hydrolysis activity
                     [Evidence IEA]"
                     /codon_start=3
                     /transl_table=11
                     /product="HAD-IC family P-type ATPase"
                     /protein_id="HOQ53964.1"
                     /translation="AIADPIKETTPAAVEALRAEGIEVVMLTGDNRVTAEAVARRLGI
                     DRVEAEVLPDHKADVVKQLREDGRVVAMAGDGVNDAPALAAADVGLAMGSGTDVAIES
                     AGITLLKGDLTGIVRARKLSQATMRNIRQNLVFAFMYNVAGIPIAAGVLYPAVGLLLS
                     PMIAAAAMALSSVSVIGNALRLRAQRL"
     gene            737..1078
                     /locus_tag="PLF56_10170"
     CDS             737..1078
                     /locus_tag="PLF56_10170"
                     /inference="COORDINATES: ab initio
                     prediction:GeneMarkS-2+"
                     /note="Derived by automated computational analysis using
                     gene prediction method: GeneMarkS-2+."
                     /codon_start=1
                     /transl_table=11
                     /product="hypothetical protein"
                     /protein_id="HOQ53965.1"
                     /translation="MSLICVSVDLAACSSDTAQGKTAPPSASSAPALDHVHGLGVNPA
                     DDTLYIASHSGVYQVTQDGAVERVADRYQDTMGFAVVGPGNFLASGHPDLIESNDAAK
                     TWTALSLIFHV"
     gene            complement(1517..2743)
                     /locus_tag="PLF56_10175"
     CDS             complement(1517..2743)
                     /locus_tag="PLF56_10175"
                     /inference="COORDINATES: protein motif:HMM:NF033542.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_function: GO:0004803 - transposase activity [Evidence
                     IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="IS110 family transposase"
                     /protein_id="HOQ53966.1"
                     /translation="MLFVGDDWAEDHHDVEIVDESGRTLARRRFPEGLVGITRLHGLI
                     AEFVPEPSGGEVLTEVVVGIETERGPWAAALVAAGYRVFAINPLSSARYRERHSTSGA
                     KSDAGDAHILAEIVRLDRAHHRQVAGDSALVEAVKLTARTHQSLIWDRTRQVLRLRSA
                     LRESFPAALKAYGELDAADTLQLLRLAPDPDQAARLTKRQVLAALARAKRRNPEVVAD
                     QIMAVLRADELRQATPVQAAFAAIVTAQVEVIATLNDQIDTLGEVVADHFGRHPDAEL
                     ITSLPGLGVILAARVLGEFGDDPSRYVDAKARKAYAGTAPITRASGIKKVVMARYARN
                     KRLGDALQQWAFCSMRGSAGAKAYYKELRGRKIGHQAALRQLANRWVGILHGCLKTGQ
                     PYNETLAWNHHTTAAA"
     gene            3128..>3619
                     /locus_tag="PLF56_10180"
     CDS             3128..>3619
                     /locus_tag="PLF56_10180"
                     /inference="COORDINATES: ab initio
                     prediction:GeneMarkS-2+"
                     /note="Derived by automated computational analysis using
                     gene prediction method: GeneMarkS-2+."
                     /codon_start=1
                     /transl_table=11
                     /product="hypothetical protein"
                     /protein_id="HOQ53967.1"
                     /translation="MGEPSKYEQVAWDEIQAFRHSWWRTTGQRLNDKAAELVSKAARD
                     AGEFVERHPKLQAGFNRASKVAKRAAGTVSKGVPYEAKEWFGKAAHSAGVTVRDISRI
                     GLTPKRIIAKHQKKGNQVETLLDIRSLDLELVDKVRGGNIVLNFLYPLTAGGSGAGSA
                     LVIS"
BASE COUNT          662 a         1233 c         1141 g          583 t
ORIGIN      
        1 tcgccatcgc cgaccccatc aaggagacca ccccggcggc cgtcgaggcg ctgcgcgccg
       61 aaggcatcga ggtcgtcatg ctcaccggcg acaaccgggt caccgccgaa gcggtggcgc
      121 gtcgccttgg catcgaccgc gtcgaggccg aggtcctgcc cgaccacaag gcagatgtcg
      181 tcaaacaact ccgtgaggac ggtcgcgtcg tcgccatggc cggcgacggc gtcaacgacg
      241 ccccagccct ggctgccgcc gacgtgggtc tggccatggg atcgggcact gacgtggcca
      301 tcgagagcgc ggggatcacg ctgctgaagg gcgacctgac cgggatcgtg cgagcacgaa
      361 agctctccca ggcaacgatg cggaacatcc ggcagaacct ggtcttcgcc ttcatgtaca
      421 acgtcgccgg catccccatc gcggcagggg ttttgtaccc cgccgtcggg ctgctgctct
      481 caccgatgat cgcagccgcg gcgatggcgc tgtcatcagt cagcgtcatc ggcaacgcac
      541 tgcgcctgcg cgcccagcgc ctataatcgg cactggcgca ccgtgcacga ccaaccactg
      601 caatccttcc ggggccgcgg cccaacgacg tcgccatctg ccgatgacca gaggcctggc
      661 ccgcatccgt ggccgacatc ggcacacccc agatgagcat cggagtcacc gtgttccaaa
      721 gcatgacctc gctgccgtgt cattgatctg cgtcagcgta gatctcgcgg catgctcctc
      781 agatacggct cagggcaaga ccgcaccacc gtcagcatcc tcggcgccgg cactggacca
      841 cgttcacggg ctcggcgtta atcctgccga cgacaccttg tacatcgcct cccactccgg
      901 ggtctaccag gtcacgcagg acggagccgt cgaacgtgtc gcggatcggt accaagacac
      961 gatggggttt gccgtcgtcg ggccgggaaa cttcctggcc agcgggcacc ccgacctgat
     1021 cgagtccaac gacgcagcca agacgtggac cgccctctcg ctcattttcc atgtctgacc
     1081 gtgatcgttg gaccccgtcc cggctgccag tggcaaaccc tgaacgggtg gacgtaccgg
     1141 atagcgtccc acaagtagga acgataaacg acgtgcgaag acggcactgg tcctgggttg
     1201 aagcacgcca agtggtgctg tcgagcacgg cgaattggtc cccggactac ggcgtccttc
     1261 gctgccagcc ccgtcgaaga cgtcgaggcg cccaacgccc tcgaaaccag catcctagcc
     1321 accctcgacc caccccctca acctcgacaa gcggccgaaa cgagatgaga acccgttcca
     1381 gcgccctcgc cgccaacaca accgcaagtg agcgcgccca acgaatgact cgcgccattt
     1441 ggcgctctgc acttgccggt ccagccgaca ggctaccctc gcgcaccgtg cggcagacat
     1501 cccaggtttt tcgatgtcaa gcagcggcgg tggtgtggtg gttccaggcg agggtttcgt
     1561 tgtagggctg gccggtcttg aggcagccat ggaggatgcc gacccagcgg ttggcgagtt
     1621 ggcgcagcgc ggcttggtgg ccgatcttcc ggccccggag ttccttgtag taggccttgg
     1681 cgccggcgga gccgcgcatc gagcagaacg cccattgctg cagggcgtca ccgagtcgtt
     1741 tgttgcgggc gtagcgggcc atcacgacct tcttgattcc tgaggccctg gtgatcgggg
     1801 cggtgccggc gtaggccttg cgggccttcg cgtcgacgta gcggctgggg tcgtcgccga
     1861 actcgccgag gacccgggcg gcgaggatca caccgaggcc gggcaggctg gtgatcagct
     1921 cagcgtccgg gtgacgacca aaatggtcgg ccaccacctc accgagagtg tcgatctggt
     1981 cattcagggt ggcgatgacc tcgacctggg ccgtgacgat cgcagcaaac gcggcctgca
     2041 cgggagttgc ctgccgcaac tcgtcggccc gcaggacggc catgatctgg tcggcgacca
     2101 cctcagggtt gcgccgcttc gccctggcca gcgcggccag gacttggcgt ttggtgagcc
     2161 gggcggcctg gtccggatca ggtgccagcc gcagcagctg caacgtgtcg gccgcgtcca
     2221 gctcgccata cgccttcaac gcggccggaa aggactcccg caacgccgaa cgaagacgca
     2281 acacctgcct ggttcggtcc caaatcaacg actggtgggt ccgggccgtc agcttcaccg
     2341 cctccaccaa cgcgctgtca ccagccacct gccggtgatg tgcccggtcg agccggacga
     2401 tctcggccag gatgtgcgca tcgccggcat ccgacttcgc ccccgacgtc gaatgccgtt
     2461 cccgatatcg cgccgacgac agcgggttga tcgcaaacac ccgatagccg gcggccacca
     2521 gggctgcggc ccacgggcca cgttcggtct cgatccccac caccacctca gtgagcacct
     2581 cgccccccga cggctcgggc acgaactccg cgatcaaccc gtgcagccgg gtaatcccga
     2641 ccagcccttc agggaatctc cgtctggcca gtgtccggcc ggactcatcg acgatctcga
     2701 cgtcgtggtg gtcctcggcc cagtcatcac caacgaacaa catgcccgcc tcctggttgc
     2761 tcgatccagc agtcagcagc gcggaggaga cgactcggcg atctaatggc ccaagtgctc
     2821 acccacaacc agccgggggc acgtcatccc agcagcgatc cccgcctcct cacgaccagc
     2881 aggcgcacag tctgccgcca gacctaccca aacggggtca gaaaagagat gagtgctgac
     2941 ccgccagccg ctaccagaac caagcctccc agagacccgg cagaaaccca ttagaatcgg
     3001 acgttacgag tgagctggct gaggcgccga ccggtggcgt gcacgaccca gacgcccggt
     3061 ttgccctcgc cagccactgt cgtcatgtct caggaacatg tcggtttgcg gccgtatagt
     3121 ccggcgcatg ggggaacctt cgaagtacga gcaggtcgcc tgggacgaga tccaggcttt
     3181 tcggcacagc tggtggagga ctactggcca gcgcctcaat gacaaggcgg ctgagcttgt
     3241 gagcaaggcg gctcgcgatg ctggcgagtt cgttgagagg catccgaagc tgcaggcggg
     3301 ttttaaccgt gccagcaagg tcgccaagcg tgcggccggc acggtcagca aaggggttcc
     3361 gtacgaggcg aaggagtggt tcgggaaggc cgcccattct gcgggtgtga ctgtccggga
     3421 catttcgcga atcgggctga ctccgaagcg gataatcgcg aagcaccaga agaagggcaa
     3481 ccaggtggag accctgctgg acatccgcag ccttgacctg gaactggtgg acaaggttcg
     3541 cggcggcaac atcgtgctca attttctcta ccccttgact gccggcggtt cgggggccgg
     3601 ctcggcgttg gtcatctca
//
LOCUS       DAOSUG010000315         3618 bp    DNA     linear   ENV 10-JUN-2024
DEFINITION  MAG TPA_asm: Micropruina sp. isolate AMR_MDS_2195
            NODE_10566_length_3618_cov_3.672748, whole genome shotgun sequence.
ACCESSION   DAOSUG010000315 DAOSUG010000000
VERSION     DAOSUG010000315.1
DBLINK      BioProject: PRJNA925477
            BioSample: SAMN32864262
            Sequence Read Archive: SRR4236673
KEYWORDS    WGS; ENV; Metagenome Assembled Genome; MAG; Third Party Data; TPA;
            TPA:assembly.
SOURCE      Micropruina sp. (wastewater metagenome)
  ORGANISM  Micropruina sp.
            Bacteria; Bacillati; Actinomycetota; Actinomycetes;
            Propionibacteriales; Nocardioidaceae; Micropruina.
REFERENCE   1  (bases 1 to 3618)
  AUTHORS   Abdulkadir,N., Saraiva,J.P., Zhang,J., Stolte,S., Gillor,O.,
            Harms,H. and Rocha,U.
  TITLE     Genome-centric analyses of 165 metagenomes show that mobile genetic
            elements are crucial for the transmission of antimicrobial
            resistance genes to pathogens in activated sludge and wastewater
  JOURNAL   Microbiol Spectr 12 (3), e0291823 (2024)
   PUBMED   38289113
REFERENCE   2  (bases 1 to 3618)
  AUTHORS   Abdulkadir,N.
  TITLE     Direct Submission
  JOURNAL   Submitted (24-MAR-2023) Environmental Microbiology, Helmholtz
            Center for Environmental Research -UFZ, Permoserstr 15, Leipzig
            04318, Germany
COMMENT     The annotation was added by the NCBI Prokaryotic Genome Annotation
            Pipeline (PGAP). Information about PGAP can be found here:
            https://www.ncbi.nlm.nih.gov/genome/annotation_prok/
            
            ##Genome-Assembly-Data-START##
            Assembly Method        :: SPAdes v. 3.11.1
            Genome Representation  :: Full
            Expected Final Version :: Yes
            Genome Coverage        :: Not Applicable
            Sequencing Technology  :: Illumina MiSeq
            ##Genome-Assembly-Data-END##
            
            ##Genome-Annotation-Data-START##
            Annotation Provider               :: NCBI
            Annotation Date                   :: 05/04/2023 04:57:10
            Annotation Pipeline               :: NCBI Prokaryotic Genome
                                                 Annotation Pipeline (PGAP)
            Annotation Method                 :: Best-placed reference protein
                                                 set; GeneMarkS-2+
            Annotation Software revision      :: 6.5
            Features Annotated                :: Gene; CDS; rRNA; tRNA; ncRNA
            Genes (total)                     :: 2,776
            CDSs (total)                      :: 2,750
            Genes (coding)                    :: 2,714
            CDSs (with protein)               :: 2,714
            Genes (RNA)                       :: 26
            tRNAs                             :: 24
            ncRNAs                            :: 2
            Pseudo Genes (total)              :: 36
            CDSs (without protein)            :: 36
            Pseudo Genes (ambiguous residues) :: 0 of 36
            Pseudo Genes (frameshifted)       :: 2 of 36
            Pseudo Genes (incomplete)         :: 32 of 36
            Pseudo Genes (internal stop)      :: 2 of 36
            ##Genome-Annotation-Data-END##
FEATURES             Location/Qualifiers
     source          1..3618
                     /organism="Micropruina sp."
                     /mol_type="genomic DNA"
                     /submitter_seqid="NODE_10566_length_3618_cov_3.672748"
                     /isolate="AMR_MDS_2195"
                     /isolation_source="Activated sludge of municipal
                     wastewater treatment plants"
                     /db_xref="taxon:2737536"
                     /environmental_sample
                     /geo_loc_name="Canada"
                     /lat_lon="49.25 N 123.1 W"
                     /collection_date="2015-06-16"
                     /metagenome_source="wastewater metagenome"
                     /note="metagenomic"
     gene            complement(<1..321)
                     /locus_tag="PLF56_10185"
     CDS             complement(<1..321)
                     /locus_tag="PLF56_10185"
                     /inference="COORDINATES: ab initio
                     prediction:GeneMarkS-2+"
                     /note="Derived by automated computational analysis using
                     gene prediction method: GeneMarkS-2+."
                     /codon_start=1
                     /transl_table=11
                     /product="hypothetical protein"
                     /protein_id="HOQ53968.1"
                     /translation="MIAVVVVGALLGAVLFALLLRLAPPTAAPLVQLAHFDAGHDAPA
                     TAPLGVGPVVSAIGGTSAPPGGAGRGNLLTRVQSSSGWWLSRQLTRRGIGYTSLRQDL
                     ALTGR"
     gene            complement(318..1283)
                     /locus_tag="PLF56_10190"
     CDS             complement(318..1283)
                     /locus_tag="PLF56_10190"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_007077373.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="type II secretion system F family protein"
                     /protein_id="HOQ53969.1"
                     /translation="MTGTVVLTVLLGIGLGVGLLLVVAGLRGTRVDPTRPPSRAARAV
                     AALRSPAVSARILAAAVVGVAVLALTRWPVAAAGLAALVLLWPRLFGGGAAEARQIAS
                     LEALVVWTESLRDTIAAHASLEHAIPASAVNAPDLIRAPLVRLVGQIRARVPLDRALL
                     NLAAELNDPSADLVIAALILNVRRRGDRLGEVLTGLASSAREELEMRRKISAGRVQLR
                     RGMQMVVAVTLGIAVFLVVFSRAYIAPYSTVAGQIALAVVVCIFGAAFAWMRKLSAQP
                     PVPPFLTRPGAQPDPFEAQLLTTLVDDPTPSTTMSTGRLRRPVIR"
     gene            complement(1280..2638)
                     /locus_tag="PLF56_10195"
     CDS             complement(1280..2638)
                     /locus_tag="PLF56_10195"
                     /inference="COORDINATES: protein motif:HMM:NF012652.2"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="ATPase, T2SS/T4P/T4SS family"
                     /protein_id="HOQ53970.1"
                     /translation="MSEMPVEYELVKALRVQVADAMTRVKQDRERRGDPELSEPDERQ
                     LALSLITSAVQAYQAEIVKAGGELPADTGYDLRLIMAVDAAIYAAGELQELLDDDQVE
                     NIDINGCDEVWITYADRGKVRGAPIAATDEDLIGIVQNLAAYASINARPFTRANPELD
                     LRLPDGSRLSAVMGAGERPAVSIRRNRYPQMFLAQLIELGTIDEQLAVFLQAAVRARM
                     NLIIAGATDAGKTTLLRACLNCIPAEERLITVERSLELGLRRQGLHADVVEWEEVLPD
                     AEGHGGISIQQLVRRTRRHNPSRVCVGEVLGPEVVEMLSAMSQGNNGSLSTLHARSAA
                     DVFAKLAQYAEQYERIENRVAHALIAGAVDFVVFIGKNRRLGGRRTVTEVLELAGAPD
                     GRVAFTPIFAPSPVDRRAVRIDEFGISEARLEELADAGYDDTAASFTFLPELSGPVGA
                     LA"
     gene            complement(2635..3531)
                     /locus_tag="PLF56_10200"
     CDS             complement(2635..3531)
                     /locus_tag="PLF56_10200"
                     /inference="COORDINATES: ab initio
                     prediction:GeneMarkS-2+"
                     /note="Derived by automated computational analysis using
                     gene prediction method: GeneMarkS-2+."
                     /codon_start=1
                     /transl_table=11
                     /product="hypothetical protein"
                     /protein_id="HOQ53971.1"
                     /translation="MIVSVCSDKGAPGVTTLATALGLVWPGERAVVEADTAGSDLSFR
                     LRQNVSEFAPPHLAPDPSIAGLATAARLGLTAAGPLPFAQDTTLGVPVVPGVLSAERF
                     RALRSLWPGLAPALARWRGTVLADLGRLHQGAPTVPVAQASTAVLLVTRADLEGLFHV
                     RDRVAELAGMVGPGEGDRVRVGVVVTAPVKQRRPAVEQVKQVLASIGSPAPVLGCFVH
                     DPAAAQSLWAGVVTRRLAGSELVRSARSVAESVLATWPSLAATATGSIGSDSSDGSGG
                     PSEQQTTDSTGVAAQQGQVVGR"
BASE COUNT          556 a         1427 c         1204 g          431 t
ORIGIN      
        1 ccggccggtc aacgccaggt cctgccgcaa gctggtgtaa ccgatcccgc ggcgggtcaa
       61 ctgccgcgac agccaccacc cggacgagct ctgcacccgg gtcagcaggt tgccgcggcc
      121 ggccccaccc ggcggcgccg aagtgcctcc gatcgccgag accaccgggc ctacccccag
      181 cggggcggtg gccggggcgt cgtggccggc gtcgaaatgg gccagctgca ccagcggcgc
      241 cgcggtgggc ggcgcgagcc gcagcagcaa ggcgaacagc acagcgccga gcagggcgcc
      301 cacgaccacg accgcgatca ccggatcacc ggccgccgca gccgcccggt gctcatggtg
      361 gtgctcgggg tggggtcgtc gaccagggtg gtcaacagct gcgcctcgaa cgggtcgggt
      421 tgcgcaccgg ggcgggtcag gaacggcggc accggcggct gcgccgagag cttgcgcatc
      481 caggcgaacg cggcgccgaa gatgcagacc acgacggcca acgcgatctg cccggcgacc
      541 gtgctgtacg gggcgatgta ggcgcggctg aacaccacca ggaacaccgc gatgcccagc
      601 gtcaccgcga caaccatctg catgccgcgg cgcagctgca cgcggccggc ggaaatcttg
      661 cgccgcatct ccagctcctc ccgggccgag gacgccaacc cggtcagcac ctcgccgagg
      721 cggtccccgc ggcgccgcac gttcaggatc agcgcggcga tgaccaggtc ggcgctcggg
      781 tcgttgagct cggcggccaa attcaatagc gcccggtcca acggcacccg agcccggatc
      841 tggccgacca ggcgaaccag cggagcccgg atcaggtccg gggcgttcac cgcggacgcg
      901 ggaatggcgt gctccaggct ggcgtgggcg gcgatggtgt cgcgcagcga ctcggtccac
      961 accaccagcg cctcgaggga ggcgatctgc cgggcctcgg cggcgccgcc gccgaacaac
     1021 cgcggccaca gcaacaccag cgcggccagc ccggcggcgg cgaccggcca ccgggtcaac
     1081 gccagcaccg cgacgcccac caccgcggcg gccaggatcc gcgcggacac cgccggtgaa
     1141 cgcagcgccg ccaccgcgcg ggcggcccgg gacggcggcc gggtcgggtc cacccgtgtt
     1201 ccgcgcagcc ccgcgaccac caacagcaga cccacgccca ggccgatgcc caacagcacc
     1261 gtcagcacca ccgtgccggt cacgccagcg cccccaccgg ccccgacagt tcaggcagga
     1321 aggtgaaaga ggcggccgtg tcgtcgtagc cggcgtcggc caactcctcc aggcgcgcct
     1381 cgctgatccc gaactcgtca atacgcaccg cccggcggtc gaccggggac ggggcgaaga
     1441 tcggggtgaa ggcgacccga ccgtccgggg cgccggccaa ctcgagcacc tcggtgacgg
     1501 tgcggcgacc gcccagccga cggttcttgc cgatgaagac cacgaagtcg accgcgccgg
     1561 cgatcagggc gtgcgcgacc cggttctcga tccgctcgta ctgctcggcg tactgggcga
     1621 gcttggcgaa cacgtccgcc gccgacctgg catggagagt ggaaagggaa ccattgttcc
     1681 cctggctcat ggccgaaagc atctcgacaa cttcgggtcc cagcacctct ccgacgcaga
     1741 cgcgggacgg gttgtgtcgc cgggtccggc ggaccaattg ctggatactg atgccgccgt
     1801 ggccctcggc gtccgggagg acctcctccc actccacgac gtcggcgtgc aggccctgcc
     1861 ggcgaagccc caactccagg gaccgttcga ccgtgatgag ccgctcctcg gccggaatgc
     1921 aattgagaca cgctctcaat agggtggttt ttccggcgtc ggtggcgccg gcgatgatca
     1981 ggttcatccg ggcccgcacc gcagcctgca ggaagacggc cagctgctcg tcgatcgtgc
     2041 ccaactcaat cagttgggcc aggaacatct gcggataccg gttccggcgg atcgacaccg
     2101 cgggccgctc accggcgccc atcacggccg acagccggga cccgtccggc agccgcaggt
     2161 ccagctccgg gttcgcccgg gtgaacgggc gggcgttgat cgacgcgtac gcggcgaggt
     2221 tctgcacgat cccgatcagg tcctcgtcgg tcgcggcgat cggcgcaccg cggaccttgc
     2281 cccggtccgc gtaggtgatc cacacctcgt cgcagccgtt gatgtcgatg ttctcgacct
     2341 ggtcgtcgtc cagcagctcc tgcagctcgc cggcggcgta gatcgccgcg tccaccgcca
     2401 tgatcagccg caggtcgtaa ccggtgtccg ccggcagctc cccgcccgcc ttgacgatct
     2461 ccgcctggta cgcctgcacc gcgctggtga tcaggctcaa cgccaactgc cgctcgtccg
     2521 gctcggacag ctcagggtcg ccccggcgtt cccggtcctg cttgacgcgg gtcatcgcgt
     2581 ccgcgacctg cacccgcagc gccttcacca gctcgtactc caccggcatc tcactcatcg
     2641 gcccaccacc tgcccctgtt gcgcggccac cccggtggag tccgtcgttt gctgctcact
     2701 cggcccgccc gagccgtccg atgagtccga tccgatcgat ccggtggcgg ttgcggccag
     2761 cgacggccag gtggccagca ccgactcggc caccgaccgg gccgaccgca ccaactcgct
     2821 gcccgccagc cggcgggtca ctaccccggc ccacagcgac tgagccgccg ccgggtcgtg
     2881 cacgaaacag cccagcaccg gcgccggcga gccgatcgac gccagcacct gtttcacctg
     2941 ttccaccgcg ggccgccgct gcttgaccgg ggcggtcacc accacgccga cccgcacccg
     3001 gtcgccctcg ccggggccga ccatcccggc cagctccgcg acccggtccc ggacgtggaa
     3061 cagtccctcc aggtccgcgc gggtcaccag cagcaccgcg gtcgaggcct gcgccaccgg
     3121 caccgtcggg gcgccctgat gcagccggcc caggtccgcc agcaccgttc cccgccaccg
     3181 ggccagggcc ggcgccaacc ccggccatag ggaccgcagc gcccggaacc gttccgccga
     3241 gagcacaccg ggcaccaccg gcacccccag cgtggtgtcc tgcgcgaacg gcaacggccc
     3301 ggccgccgtc agccccagcc gcgccgcggt ggccagaccc gcgatggagg gatccggcgc
     3361 cagatgcggc ggcgcgaact cggacacgtt ctggcgcagc cggaacgaca gatcactgcc
     3421 ggccgtgtcg gcctccacca ccgcccgctc gcccggccac accaagccca gcgccgtcgc
     3481 cagcgtcgtc accccgggag cacccttgtc cgagcacacc gacacgatca cggcgtcggc
     3541 accttcacca gcgccgccga tcccgacgcg ctcgccgccg ccaccgccgg cccgtccgcc
     3601 gccggcacca gcagcgtc
//
LOCUS       DAOSUG010000316         3616 bp    DNA     linear   ENV 10-JUN-2024
DEFINITION  MAG TPA_asm: Micropruina sp. isolate AMR_MDS_2195
            NODE_10572_length_3616_cov_5.745577, whole genome shotgun sequence.
ACCESSION   DAOSUG010000316 DAOSUG010000000
VERSION     DAOSUG010000316.1
DBLINK      BioProject: PRJNA925477
            BioSample: SAMN32864262
            Sequence Read Archive: SRR4236673
KEYWORDS    WGS; ENV; Metagenome Assembled Genome; MAG; Third Party Data; TPA;
            TPA:assembly.
SOURCE      Micropruina sp. (wastewater metagenome)
  ORGANISM  Micropruina sp.
            Bacteria; Bacillati; Actinomycetota; Actinomycetes;
            Propionibacteriales; Nocardioidaceae; Micropruina.
REFERENCE   1  (bases 1 to 3616)
  AUTHORS   Abdulkadir,N., Saraiva,J.P., Zhang,J., Stolte,S., Gillor,O.,
            Harms,H. and Rocha,U.
  TITLE     Genome-centric analyses of 165 metagenomes show that mobile genetic
            elements are crucial for the transmission of antimicrobial
            resistance genes to pathogens in activated sludge and wastewater
  JOURNAL   Microbiol Spectr 12 (3), e0291823 (2024)
   PUBMED   38289113
REFERENCE   2  (bases 1 to 3616)
  AUTHORS   Abdulkadir,N.
  TITLE     Direct Submission
  JOURNAL   Submitted (24-MAR-2023) Environmental Microbiology, Helmholtz
            Center for Environmental Research -UFZ, Permoserstr 15, Leipzig
            04318, Germany
COMMENT     The annotation was added by the NCBI Prokaryotic Genome Annotation
            Pipeline (PGAP). Information about PGAP can be found here:
            https://www.ncbi.nlm.nih.gov/genome/annotation_prok/
            
            ##Genome-Assembly-Data-START##
            Assembly Method        :: SPAdes v. 3.11.1
            Genome Representation  :: Full
            Expected Final Version :: Yes
            Genome Coverage        :: Not Applicable
            Sequencing Technology  :: Illumina MiSeq
            ##Genome-Assembly-Data-END##
            
            ##Genome-Annotation-Data-START##
            Annotation Provider               :: NCBI
            Annotation Date                   :: 05/04/2023 04:57:10
            Annotation Pipeline               :: NCBI Prokaryotic Genome
                                                 Annotation Pipeline (PGAP)
            Annotation Method                 :: Best-placed reference protein
                                                 set; GeneMarkS-2+
            Annotation Software revision      :: 6.5
            Features Annotated                :: Gene; CDS; rRNA; tRNA; ncRNA
            Genes (total)                     :: 2,776
            CDSs (total)                      :: 2,750
            Genes (coding)                    :: 2,714
            CDSs (with protein)               :: 2,714
            Genes (RNA)                       :: 26
            tRNAs                             :: 24
            ncRNAs                            :: 2
            Pseudo Genes (total)              :: 36
            CDSs (without protein)            :: 36
            Pseudo Genes (ambiguous residues) :: 0 of 36
            Pseudo Genes (frameshifted)       :: 2 of 36
            Pseudo Genes (incomplete)         :: 32 of 36
            Pseudo Genes (internal stop)      :: 2 of 36
            ##Genome-Annotation-Data-END##
FEATURES             Location/Qualifiers
     source          1..3616
                     /organism="Micropruina sp."
                     /mol_type="genomic DNA"
                     /submitter_seqid="NODE_10572_length_3616_cov_5.745577"
                     /isolate="AMR_MDS_2195"
                     /isolation_source="Activated sludge of municipal
                     wastewater treatment plants"
                     /db_xref="taxon:2737536"
                     /environmental_sample
                     /geo_loc_name="Canada"
                     /lat_lon="49.25 N 123.1 W"
                     /collection_date="2015-06-16"
                     /metagenome_source="wastewater metagenome"
                     /note="metagenomic"
     gene            74..301
                     /locus_tag="PLF56_10205"
     CDS             74..301
                     /locus_tag="PLF56_10205"
                     /inference="COORDINATES: protein motif:HMM:NF015956.2"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="ferrous iron transport protein A"
                     /protein_id="HOQ53972.1"
                     /translation="MTALTLAEAPLATTLTLSDVPQGQARHLSRLGLRPGAALQVIRR
                     STGGGRIVSVAGARVALGRGLLAGMQVEVGR"
     gene            298..2382
                     /gene="feoB"
                     /locus_tag="PLF56_10210"
     CDS             298..2382
                     /gene="feoB"
                     /locus_tag="PLF56_10210"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_015071061.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_function: GO:0015093 - ferrous iron transmembrane
                     transporter activity [Evidence IEA];
                     GO_process: GO:0006826 - iron ion transport [Evidence
                     IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="ferrous iron transport protein B"
                     /protein_id="HOQ53973.1"
                     /translation="MSSEPNRRAGATERHGTDLYADGRVDPADGNTLAAIDGDGARAV
                     GTSVRSATGTARRTTPVTTCHCAVANTGAGAGAPVVALIGAPNSGKSTLFNALTGSRV
                     TVGNWPGTTVEVSRGVWSQRTLLDLPGAYSLDPLSPDEELTRDLLLTDPDDRPDLVIV
                     VADAANLARGLYLVSQVRENRQRVIVALTMLDVAHRRGIEIDTDALSQALGCPVVELD
                     PRRREGLGALNTAVAASLDAPEPRRRPHDCCADELEKADERFAWVAAAVEAGALDPGE
                     ERVSFSDRLDRVATAPVLGPLLFLGVMWLVFQVTTTVAAPIQDFFDQLFSGPLTDGAL
                     AGFAALGLTGSWVEGLVVNGLIAGVGMVLTFVPLMALMFVLLALLEDSGYLARAAVVT
                     DRLMRSIGLPGKAFLPLVVGFGCNVPAISATRILGDGRQRLLTALLVPFTSCTARLTV
                     FVLVSTVFFGPWAGTVVFGMYVSSILLIVGTGLLLRRTLWRAMGTEPLVIDLPPYQLP
                     TLRLTAAVTWTRLQGFLRTASGIIVATVCAVWLLQSIPAHGVGGFGQVDVEDSVYGVA
                     AQAITPVFEPAGFAQWQTTSALVVGFVAKEAVISSWAQTYALDEPEGQDPGSLGDRIL
                     VDFAQSSGGHPIPAALAFMIFLMAYTPCVATLAAQVREIGWRWTGFGIALQLAIAWGL
                     AVVVFQVGSLWW"
     gene            2373..2597
                     /locus_tag="PLF56_10215"
     CDS             2373..2597
                     /locus_tag="PLF56_10215"
                     /inference="COORDINATES: ab initio
                     prediction:GeneMarkS-2+"
                     /note="Derived by automated computational analysis using
                     gene prediction method: GeneMarkS-2+."
                     /codon_start=1
                     /transl_table=11
                     /product="hypothetical protein"
                     /protein_id="HOQ53974.1"
                     /translation="MVVAGPLTTVLGALSDGAGSLGEVSRRTGLRLDVVRAATDHLVR
                     LGRVQQCPLSIGCAAGGCGGCPKLSDGRCG"
     gene            2719..3291
                     /locus_tag="PLF56_10220"
     CDS             2719..3291
                     /locus_tag="PLF56_10220"
                     /inference="COORDINATES: protein motif:HMM:NF015674.2"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="DUF308 domain-containing protein"
                     /protein_id="HOQ53975.1"
                     /translation="MSNTFLTQDFGEVDKRISNGIRSALGVGGLVSLIVGIVILVWPG
                     KSAMVVAGIIAVWALIAGLVNIAIGIFSRALGTWPRIGYLVLGVLFLVAAVITFGNLG
                     AAAVSLGVLLGIIVGITWLIEGIVALTLLGDASSKTWTILYAVLSIIAGVIVLFSPLW
                     GAALLWLLLGVSLVILGIAQLVRAFRFGSR"
BASE COUNT          466 a         1222 c         1276 g          652 t
ORIGIN      
        1 tgccacgtag cactgtggtc aggacaggct gtgcttcgtt ccagctttct cgaccgagga
       61 ttggcgaccc gacatgactg ccctcacgct ggcggaggct ccgctggcga ccaccctcac
      121 actgtccgac gtgccgcagg gccaggcccg gcatctgagt cggctggggc ttcgtccggg
      181 ggcggcgttg caggtgatcc gtcgcagcac cggtggcgga cgcatcgtct cggtcgccgg
      241 cgcccgggtg gcgttgggcc ggggactgct ggccggcatg caggtcgagg tggggcggtg
      301 agcagcgagc ccaatcgccg cgccggcgcg accgagcgcc acggcaccga cctgtatgcc
      361 gacggccgcg tcgacccggc cgacggcaac accttggccg ccatcgacgg cgatggcgcg
      421 agggccgtgg gcacgtccgt gagaagcgcc accggcaccg cccggcgcac cacgccggtc
      481 accacctgtc attgcgcggt ggccaacacg ggtgcgggcg cgggtgcgcc ggtggtggcg
      541 ctgatcggcg ccccgaactc gggcaagtcg accctgttca acgcgctcac cggttcgcgc
      601 gtcacggtcg gcaactggcc cggcaccacc gtcgaggtgt cgcgcggcgt ctggtcgcaa
      661 cgcaccctgc tcgacctgcc cggcgcctac tcgctcgacc cgctctcacc cgatgaggag
      721 ctgacccgcg atctgctgct gaccgatccc gacgaccgtc cggatctggt gatcgtggtg
      781 gctgatgccg ccaacctggc ccgtggcctg tacctggtca gccaggtgcg agagaaccgc
      841 cagcgggtga tcgtcgccct caccatgctc gacgtcgccc accggcgcgg catcgagatc
      901 gacaccgacg cgctcagcca ggcgctcggc tgccccgtgg ttgagctgga tccccggcgc
      961 cgcgagggcc tcggcgccct gaacacggcc gtggcggcgt cgctcgatgc gcccgaaccc
     1021 cgccgccgcc cgcacgactg ctgcgccgac gaactagaga aggcggacga acggttcgcc
     1081 tgggtcgctg cggccgtcga ggccggcgca ctcgaccccg gcgaggagcg cgtctcgttc
     1141 tcggatcggc tcgaccgcgt ggcgaccgcg ccggtgctcg gcccgctgct gtttctgggt
     1201 gtgatgtggc tggtgttcca ggtcaccacc acggtggccg cgccgatcca ggacttcttc
     1261 gaccagttgt tctcgggccc tctcaccgac ggtgccctgg ccggcttcgc tgccctcggc
     1321 ctgaccggct cgtgggtcga ggggctggtg gtcaacggcc tgatcgccgg cgtcggcatg
     1381 gtgctcacct tcgtgccgct gatggcgctg atgttcgtgc tgctggcact gctggaggac
     1441 tcgggctacc tggcccgcgc cgccgtggtg accgaccgcc tgatgcgttc gatcggcctg
     1501 ccgggcaagg cctttctgcc cctggtggtg ggtttcggct gcaacgtgcc ggcgatcagc
     1561 gcaacccgca ttttgggcga tggacggcag cggctgctca ccgcgctgct ggtgccgttc
     1621 acctcgtgca ccgcccggct gacggtgttc gtgttggtgt caaccgtgtt cttcggcccg
     1681 tgggccggca cggtggtgtt cggcatgtac gtgtcgtcca ttctgctgat agtcggcacc
     1741 gggctgctgc tgcgacgcac cctgtggcgc gccatgggca ccgagccgct ggtgatcgac
     1801 ctgccgccct accaactgcc gacccttcga ctgaccgccg cggtgacgtg gacccggctg
     1861 cagggctttc tgcgcaccgc gtccggcatc atcgtggcga ccgtgtgtgc ggtgtggctg
     1921 ctgcagtcca ttccggcgca cggggtgggt ggtttcggcc aggtcgatgt cgaggattcg
     1981 gtgtacggcg tcgccgcgca ggcgatcacc ccggtcttcg agccggcagg tttcgcgcag
     2041 tggcagacca cgtccgcgct ggtggtcggg ttcgtcgcca aagaggcggt gatcagctcg
     2101 tgggcccaga cctatgccct ggacgagccc gaaggccagg atcccggctc gctgggcgat
     2161 cgcattctgg tcgacttcgc gcagagttcg ggtggtcacc cgataccggc ggcgttggcg
     2221 ttcatgatct ttctgatggc ctacaccccg tgcgtggcca cgctggccgc acaggtgcga
     2281 gagatcggct ggcgctggac cggcttcggt atcgcactgc agctcgcgat cgcctggggt
     2341 ctcgcggtgg tcgtgtttca ggtggggagc ctgtggtggt agccggaccg ctcaccaccg
     2401 tcctgggcgc actgagtgac ggcgccggct cgctcggcga ggtgagccgc cgcaccggtc
     2461 tgcgcctcga cgtggtacgc gccgccaccg accacctggt gcgcctgggg cgggtgcagc
     2521 agtgtccgct gagcatcggc tgcgcggcag gtggctgcgg tggctgtccc aagctgagcg
     2581 acggacggtg cggctagcct gcgttcgcac gtgatgcgag ctcacatcgg cacggctccc
     2641 acgattgcac tcagcggtgc gagaaactgt gaatgcggca aggcccgcca gagccaatat
     2701 gttccaagga gtttcaccat gagtaataca ttcctgacgc aggacttcgg cgaagtggac
     2761 aagagaatca gcaacggtat tcgttcggca ctgggggtcg gcggcctggt gtcgttgatc
     2821 gtcggcatcg tgattctggt ctggcccggc aagtcggcca tggtggtggc cggcatcatc
     2881 gcggtctggg ccctgatcgc gggtctggtg aacatcgcca tcggcatctt cagccgcgcc
     2941 ctgggcacct ggccccgcat cgggtacttg gtgctcggcg tgctgttcct ggttgctgcg
     3001 gtgatcacct tcggcaacct cggcgccgcc gcggtcagtc tcggcgttct gctcggcatc
     3061 atcgtcggca tcacctggct catcgaaggc attgtcgcgc tcacgctgtt gggtgacgcc
     3121 tcatcgaaga cctggacgat cctgtatgcg gtgctgagca tcatcgccgg cgtcatcgtg
     3181 ctgttctccc cgctgtgggg cgccgccctg ctgtggctgc tgctcggcgt gtcgctggtc
     3241 atcttgggca tcgcgcaact ggtgcgcgcg ttccggttcg gtagccggta gccggtagcc
     3301 ggtagccggt agccggtagc cgccccgcga gggcggcacg caatacaact ctcgaccgtc
     3361 atcccggcgc acgccgggat ctctgcttac gccgggtctg cgcgtgccga gatcccgggg
     3421 caggcccggg atgacggcga agagggtggc cgaaacccct agccgcaccg gtcacgataa
     3481 gcgtggcgac cttccgccat cccggcgcat gccgggatct ctgcttacgc cgggtttgcg
     3541 cgtgccgaga tcccggggcg agcccgggat gacggcgaag agggtggccg aaacccctag
     3601 ccgcaccggt cacgat
//
LOCUS       DAOSUG010000317         3614 bp    DNA     linear   ENV 10-JUN-2024
DEFINITION  MAG TPA_asm: Micropruina sp. isolate AMR_MDS_2195
            NODE_10577_length_3614_cov_3.719865, whole genome shotgun sequence.
ACCESSION   DAOSUG010000317 DAOSUG010000000
VERSION     DAOSUG010000317.1
DBLINK      BioProject: PRJNA925477
            BioSample: SAMN32864262
            Sequence Read Archive: SRR4236673
KEYWORDS    WGS; ENV; Metagenome Assembled Genome; MAG; Third Party Data; TPA;
            TPA:assembly.
SOURCE      Micropruina sp. (wastewater metagenome)
  ORGANISM  Micropruina sp.
            Bacteria; Bacillati; Actinomycetota; Actinomycetes;
            Propionibacteriales; Nocardioidaceae; Micropruina.
REFERENCE   1  (bases 1 to 3614)
  AUTHORS   Abdulkadir,N., Saraiva,J.P., Zhang,J., Stolte,S., Gillor,O.,
            Harms,H. and Rocha,U.
  TITLE     Genome-centric analyses of 165 metagenomes show that mobile genetic
            elements are crucial for the transmission of antimicrobial
            resistance genes to pathogens in activated sludge and wastewater
  JOURNAL   Microbiol Spectr 12 (3), e0291823 (2024)
   PUBMED   38289113
REFERENCE   2  (bases 1 to 3614)
  AUTHORS   Abdulkadir,N.
  TITLE     Direct Submission
  JOURNAL   Submitted (24-MAR-2023) Environmental Microbiology, Helmholtz
            Center for Environmental Research -UFZ, Permoserstr 15, Leipzig
            04318, Germany
COMMENT     The annotation was added by the NCBI Prokaryotic Genome Annotation
            Pipeline (PGAP). Information about PGAP can be found here:
            https://www.ncbi.nlm.nih.gov/genome/annotation_prok/
            
            ##Genome-Assembly-Data-START##
            Assembly Method        :: SPAdes v. 3.11.1
            Genome Representation  :: Full
            Expected Final Version :: Yes
            Genome Coverage        :: Not Applicable
            Sequencing Technology  :: Illumina MiSeq
            ##Genome-Assembly-Data-END##
            
            ##Genome-Annotation-Data-START##
            Annotation Provider               :: NCBI
            Annotation Date                   :: 05/04/2023 04:57:10
            Annotation Pipeline               :: NCBI Prokaryotic Genome
                                                 Annotation Pipeline (PGAP)
            Annotation Method                 :: Best-placed reference protein
                                                 set; GeneMarkS-2+
            Annotation Software revision      :: 6.5
            Features Annotated                :: Gene; CDS; rRNA; tRNA; ncRNA
            Genes (total)                     :: 2,776
            CDSs (total)                      :: 2,750
            Genes (coding)                    :: 2,714
            CDSs (with protein)               :: 2,714
            Genes (RNA)                       :: 26
            tRNAs                             :: 24
            ncRNAs                            :: 2
            Pseudo Genes (total)              :: 36
            CDSs (without protein)            :: 36
            Pseudo Genes (ambiguous residues) :: 0 of 36
            Pseudo Genes (frameshifted)       :: 2 of 36
            Pseudo Genes (incomplete)         :: 32 of 36
            Pseudo Genes (internal stop)      :: 2 of 36
            ##Genome-Annotation-Data-END##
FEATURES             Location/Qualifiers
     source          1..3614
                     /organism="Micropruina sp."
                     /mol_type="genomic DNA"
                     /submitter_seqid="NODE_10577_length_3614_cov_3.719865"
                     /isolate="AMR_MDS_2195"
                     /isolation_source="Activated sludge of municipal
                     wastewater treatment plants"
                     /db_xref="taxon:2737536"
                     /environmental_sample
                     /geo_loc_name="Canada"
                     /lat_lon="49.25 N 123.1 W"
                     /collection_date="2015-06-16"
                     /metagenome_source="wastewater metagenome"
                     /note="metagenomic"
     gene            complement(<1..1913)
                     /gene="purL"
                     /locus_tag="PLF56_10225"
     CDS             complement(<1..1913)
                     /gene="purL"
                     /locus_tag="PLF56_10225"
                     /EC_number="6.3.5.3"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_013861586.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_function: GO:0004642 -
                     phosphoribosylformylglycinamidine synthase activity
                     [Evidence IEA];
                     GO_process: GO:0006189 - 'de novo' IMP biosynthetic
                     process [Evidence IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="phosphoribosylformylglycinamidine synthase
                     subunit PurL"
                     /protein_id="HOQ53976.1"
                     /translation="MVDTVAHAAETPDLEQPWAALGLRENEYHRIREILGRRPTSCEL
                     AMYSVMWSEHCSYKSSKVHLKKFGVLGRETPRGKLLAGIGEQAGVVDIGGDWAVTFKI
                     ESHNHPSYVEPYQGAATGVGGIVRDILAMGARPIGVMDPLRFGPLDAPDTARVLPGIV
                     AGVGGYGNCLGLPNIGGEVVFHPTYYGNPLVNALCVGVLKHDDLHLAFARGTGNKVIL
                     YGAATGGDGIGGASILASETFDADGPAKRPSVQVGDPFMEKLLIECTLELFAENLISG
                     IQDLGAAGLSCATSELASAGDGGMRVNLNTVPLRDSTLAPEEILMSESQERMMAVVEP
                     HNVERFLEICAKWDVLATVVGEVTDGDRLIIEWNGETVVDVDPVTVAHEGPTYERPYA
                     RPDWIDALNADTAESLPRAGTDEELADQVLRLAGSPNLCDKSWVTDQYDRYVRGNSVL
                     AQPEDGGMLRIDEETGLGIALATDCNGRFAYLNPYLGAQLALCEAYRNVAVTGAEPVA
                     ITDCLNYGSPEDPDVMWQFSEAVIALVDGCTALGTPVTGGNVSFYNQTDGANILPTPV
                     VGVLGVIDDVADRTPLGFRHAGDAVFLLGRTGENLSGSTWADVIHDGHLGGMPPIPDF
                     EHEQRVARVLVAAS"
     gene            2235..3155
                     /locus_tag="PLF56_10230"
     CDS             2235..3155
                     /locus_tag="PLF56_10230"
                     /inference="COORDINATES: protein motif:HMM:NF013986.2"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="BadF/BadG/BcrA/BcrD ATPase family protein"
                     /protein_id="HOQ53977.1"
                     /translation="MTDSSHGLLALDFGQTSLSWRVVADAGQQSGHAKGVDTSRPIEP
                     QIVDAARAILTLTGAAPSMVACGSSGLDRPLAQAALDGLQGTSVTDVALAHDSTTSYL
                     GALGDEPGVMITSGTGVVTLAVGPDDVARVDGWGWIMGDAGSAFWIGRNALEAAMRGY
                     DGRRAETALTEVVAADFDDLELAYLELQADPNRVARVASYAAKVDEVAVHDPVARNIL
                     DKAAAHLAEAVVAGAHRVGLGRGTAPPVCALGQTFGSKRLLEQFVSYLTLDWPNFALR
                     TPLGDGLAGAAMLPTVEGALAARVSRASGS"
BASE COUNT          544 a         1201 c         1277 g          592 t
ORIGIN      
        1 gaggccgcga ccagcacccg ggccacgcgc tgctcgtgct cgaagtcggg gatcggcggc
       61 atgccgccca ggtgaccgtc gtggatcacg tccgcccacg tggaccccga gagattctcg
      121 ccggtccgac cgagcaggaa caccgcgtcg ccggcatgcc tgaagcccaa cggggtgcga
      181 tcggccacgt cgtcgatgac gccgagcacc cccaccaccg gcgtgggcag aatgttggcg
      241 ccgtcggtct ggttgtagaa gctcacgttg ccgccggtga cgggggtgcc gagcgccgtg
      301 cagccgtcca ccagggcgat cacggcctcg gagaactgcc acatcacgtc gggatcctcg
      361 gggctgccgt agttgaggca gtcggtgatc gccaccggtt cagcacccgt gacggccacg
      421 ttgcgatatg cctcgcacaa ggccagttgc gcgcccaggt aggggttgag gtaggcgaag
      481 cgcccgttgc agtcggtggc cagggcgatg cccaggccgg tttcttcgtc gatgcgcaac
      541 atgccgccgt cctcgggctg ggcgagcacc gagttgcccc gcacgtagcg gtcgtactgg
      601 tcggtcaccc acgacttgtc gcacaggttc ggcgagccgg ccagtcgcag cacctggtca
      661 gccaactcct cgtcggtgcc ggccctcggc agcgactcgg cggtgtcggc gttcagtgcg
      721 tcgatccagt ccggacgggc gtagggccgc tcgtaggtcg ggccctcgtg ggcaacggtg
      781 acggggtcga cgtcgacgac ggtctcgccg ttccactcga tgatcagccg gtcgccgtcg
      841 gtgacctcgc cgacgacggt cgcgagcacg tcccacttgg cgcagatctc gaggaaccgc
      901 tcgacgttgt gcggctcgac gaccgccatc atgcgttcct gcgactcgct catcagaatc
      961 tcttcggggg ccagcgtcga gtcgcgcaac ggcaccgtgt tgaggttgac gcgcatgccg
     1021 ccgtcgccgg cgctggccag ttcggacgtg gcgcacgaga gccccgccgc accaaggtcc
     1081 tgaatgcccg agatcaggtt ctcggcgaac agctccaaag tgcattcgat caggagtttc
     1141 tccatgaacg gatcgccgac ctgcacgctg ggacgcttcg ccggcccgtc cgcgtcgaag
     1201 gtttcggagg ccagaatgga cgccccgccg atgccgtcgc cgccggtggc cgcgccgtag
     1261 aggatcacct tgttgccggt tccgcgggcg aaggccaggt gcaggtcgtc gtgcttgagc
     1321 acaccgacgc acagggcgtt gaccaggggg tttccgtagt aggtggggtg gaagaccact
     1381 tcgccgccga tgttcggcag tccgaggcag ttgccgtaac cgccgacgcc ggcgacgatg
     1441 ccgggcagca cccgcgcggt gtcgggggcg tccaggggcc cgaagcgcag cggatccatc
     1501 acgccgatcg gccgtgcacc catggcgaga atgtcgcgga cgatgccgcc cacaccggtc
     1561 gcggcgccct ggtacggctc cacgtagctc gggtggttgt gtgactcgat cttgaacgtg
     1621 accgcccagt cgccgccgat gtcgaccacc ccggcctgct cgccgatgcc ggccagcagc
     1681 ttgccgcgcg gcgtctcacg gcccagcacc ccgaacttct tcaggtgcac cttggacgac
     1741 ttgtacgagc agtgctccga ccacatcacc gagtacatgg ccagttcgca cgaggtgggc
     1801 cggcgcccga gaatctcgcg gatcctgtgg tactcgttct cgcgcagacc gagtgcggcc
     1861 cagggctgct cgagatcggg ggtttcggcg gcatgtgcaa cggtgtcgac cacgcccaca
     1921 aggtaccgtc tcgcggcggg gtttcggcga tcgtcccgct cggcgcgagg caggcgtact
     1981 cgcggtgatc cgtgggctgt ccacgttgaa acgcggttga gcgtgtcgag aaccagttga
     2041 gcgtgggcga gatctcgacg cgctcgacca acgatcgagc gcgtcggact gggaacggtc
     2101 tcgacccgct cgtccagcga gagcggtcgc ccggcgaccc ggtcgccgcc cgcgtcggac
     2161 ctgctcgagt gccgcggaac ggtgagtcat tcgggcgacg catcactaga atgctgccaa
     2221 aggcggtgat tcccatgacc gactcttcac acggcctact ggctctcgat ttcggccaaa
     2281 cctctctcag ttggcgcgtg gtcgccgacg ctgggcagca gagtggccac gcaaagggtg
     2341 tcgacacgag tcgtccgatc gaaccccaga tcgtggacgc ggcgcgcgcc attctcactc
     2401 tgaccggtgc ggccccgtcc atggtggcgt gtggatcgag cggactggac cgtccactgg
     2461 cgcaggcggc actggacggg ctgcagggaa cgtccgtcac cgacgtggcg ctggcgcacg
     2521 actcgacgac ctcgtacctc ggcgctctgg gtgacgagcc gggcgtgatg atcacctccg
     2581 gaaccggtgt ggtcacactc gccgtcggcc ccgacgacgt ggcacgggtg gacggctggg
     2641 gctggatcat gggcgatgcc ggctcggcgt tctggatcgg acgcaacgcg ctggaggccg
     2701 cgatgcgggg ctatgacggg cgccgcgcgg agaccgcgtt gaccgaggtg gtggccgcgg
     2761 acttcgacga cctcgagctg gcgtacctgg agttgcaggc ggatccgaac cgggtcgcgc
     2821 gggtggcgtc gtatgcggcg aaggtcgatg aggtggcagt ccacgacccg gtggcacgca
     2881 acattctcga caaggccgcc gcgcacctgg cggaggccgt cgtcgcgggc gcgcaccggg
     2941 tcggcctggg ccgcggcacg gccccgccgg tgtgcgcgct cggccagacg ttcggctcga
     3001 agcggttgct ggagcaattc gtctcatacc tgaccctgga ttggcccaac tttgcgctca
     3061 ggacgccgct gggcgacggg ctggcgggtg cggccatgct gcccacagtg gagggtgcgc
     3121 tggcggcgcg ggtctcacga gcctcgggtt cgtgagctga gggctggtgc gggtgcgtcc
     3181 gtgagctgag gccggcgccg tccaactcgc tcaacggaag gcctccggtg tcgcacccgt
     3241 tgatcgggca ggtcacgcgc ctgttgatca ggtaggtcaa ccgcccgttg accgggtagg
     3301 tcactcgccc cttgaccggg caggttggca cggccgttga tcgggcaggt cacgcgcctg
     3361 ttgatcaggt aggtcaaccg cccattgacc gggcaggtca cccgccgatt gatcaggcag
     3421 gtcacccgcc cattgatcag gacggtcacc cgccgattga tcaggcaggt cgcccgccga
     3481 ttgaccaggt gggtcacccg cccattgacc gggtaggtca cccgcccatt gacggggtag
     3541 gtcccccgcc cattgaccgg gtaggtcacc cgcccattga tcaggtaggg cactcgccgt
     3601 gccgggtggg cctc
//
LOCUS       DAOSUG010000318         3605 bp    DNA     linear   ENV 10-JUN-2024
DEFINITION  MAG TPA_asm: Micropruina sp. isolate AMR_MDS_2195
            NODE_10606_length_3605_cov_3.546479, whole genome shotgun sequence.
ACCESSION   DAOSUG010000318 DAOSUG010000000
VERSION     DAOSUG010000318.1
DBLINK      BioProject: PRJNA925477
            BioSample: SAMN32864262
            Sequence Read Archive: SRR4236673
KEYWORDS    WGS; ENV; Metagenome Assembled Genome; MAG; Third Party Data; TPA;
            TPA:assembly.
SOURCE      Micropruina sp. (wastewater metagenome)
  ORGANISM  Micropruina sp.
            Bacteria; Bacillati; Actinomycetota; Actinomycetes;
            Propionibacteriales; Nocardioidaceae; Micropruina.
REFERENCE   1  (bases 1 to 3605)
  AUTHORS   Abdulkadir,N., Saraiva,J.P., Zhang,J., Stolte,S., Gillor,O.,
            Harms,H. and Rocha,U.
  TITLE     Genome-centric analyses of 165 metagenomes show that mobile genetic
            elements are crucial for the transmission of antimicrobial
            resistance genes to pathogens in activated sludge and wastewater
  JOURNAL   Microbiol Spectr 12 (3), e0291823 (2024)
   PUBMED   38289113
REFERENCE   2  (bases 1 to 3605)
  AUTHORS   Abdulkadir,N.
  TITLE     Direct Submission
  JOURNAL   Submitted (24-MAR-2023) Environmental Microbiology, Helmholtz
            Center for Environmental Research -UFZ, Permoserstr 15, Leipzig
            04318, Germany
COMMENT     The annotation was added by the NCBI Prokaryotic Genome Annotation
            Pipeline (PGAP). Information about PGAP can be found here:
            https://www.ncbi.nlm.nih.gov/genome/annotation_prok/
            
            ##Genome-Assembly-Data-START##
            Assembly Method        :: SPAdes v. 3.11.1
            Genome Representation  :: Full
            Expected Final Version :: Yes
            Genome Coverage        :: Not Applicable
            Sequencing Technology  :: Illumina MiSeq
            ##Genome-Assembly-Data-END##
            
            ##Genome-Annotation-Data-START##
            Annotation Provider               :: NCBI
            Annotation Date                   :: 05/04/2023 04:57:10
            Annotation Pipeline               :: NCBI Prokaryotic Genome
                                                 Annotation Pipeline (PGAP)
            Annotation Method                 :: Best-placed reference protein
                                                 set; GeneMarkS-2+
            Annotation Software revision      :: 6.5
            Features Annotated                :: Gene; CDS; rRNA; tRNA; ncRNA
            Genes (total)                     :: 2,776
            CDSs (total)                      :: 2,750
            Genes (coding)                    :: 2,714
            CDSs (with protein)               :: 2,714
            Genes (RNA)                       :: 26
            tRNAs                             :: 24
            ncRNAs                            :: 2
            Pseudo Genes (total)              :: 36
            CDSs (without protein)            :: 36
            Pseudo Genes (ambiguous residues) :: 0 of 36
            Pseudo Genes (frameshifted)       :: 2 of 36
            Pseudo Genes (incomplete)         :: 32 of 36
            Pseudo Genes (internal stop)      :: 2 of 36
            ##Genome-Annotation-Data-END##
FEATURES             Location/Qualifiers
     source          1..3605
                     /organism="Micropruina sp."
                     /mol_type="genomic DNA"
                     /submitter_seqid="NODE_10606_length_3605_cov_3.546479"
                     /isolate="AMR_MDS_2195"
                     /isolation_source="Activated sludge of municipal
                     wastewater treatment plants"
                     /db_xref="taxon:2737536"
                     /environmental_sample
                     /geo_loc_name="Canada"
                     /lat_lon="49.25 N 123.1 W"
                     /collection_date="2015-06-16"
                     /metagenome_source="wastewater metagenome"
                     /note="metagenomic"
     gene            <1..1320
                     /locus_tag="PLF56_10235"
     CDS             <1..1320
                     /locus_tag="PLF56_10235"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_013864394.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_function: GO:0022857 - transmembrane transporter
                     activity [Evidence IEA];
                     GO_process: GO:0055085 - transmembrane transport [Evidence
                     IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="nitrate/nitrite transporter"
                     /protein_id="HOQ53978.1"
                     /translation="HWIDDWRPEDLEFWNTTGAKIARRNLIYSVASEHIGFSVWTLWS
                     VLVLFLGPDYGFDVSQKFLLTAVPSLLGALVRVPYTFAVAVFGGRNWTVISTLLLLIP
                     TVAIAIVLQPGVSFTTLLWVSALAGVGGGNFASSMTNINAFFPARLKGWALGVNAGGG
                     NLGVAVAQLIGLAVLALTGTQYPRLMVAIYIPLVLLVATLAALKMDNLGVVSNAKGAM
                     REVITHRESWVLALLYIGTFGSFIGFSFAFGQVLLTQFPKEFPNPAAAAALTFLGPLL
                     GSLIRPVGGKLADRFGGSVVTFWNFLAMGLGTALVLLAAQLHSLALFVTGFIMLFVFS
                     GLGNGSTYKMIPAIFTAKAQIAVGAGQPIEHEMAQSLRRSGALIGLAGAIGALGGFGV
                     NLAFRQSFLTMGNANGAYLVFIGFYVVCMVVTWQCFMRARAGRLEGV"
     gene            1747..3030
                     /locus_tag="PLF56_10240"
     CDS             1747..3030
                     /locus_tag="PLF56_10240"
                     /EC_number="4.2.1.75"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_007077517.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_function: GO:0004852 - uroporphyrinogen-III synthase
                     activity [Evidence IEA];
                     GO_process: GO:0006780 - uroporphyrinogen III biosynthetic
                     process [Evidence IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="uroporphyrinogen-III synthase"
                     /protein_id="HOQ53979.1"
                     /translation="MLGPILEGARVLVTAQRRADDLAAALIRRGAQVDIASTLGVETH
                     IDEAGLLERTRQLLYRPPAIVVVTTAIGLRGWLETAETSGLGAPFVELLRASRVIARG
                     PKALGALQGIGVTPDWVAESETSREILDLLSAEGVEGQRIAVQLHGAGDDGLVSGLVR
                     AGADVVPLTVYRWCSPHDPEAVLESVDLARAGAYDAVTFTSAPGAAAWIAAVESRGAL
                     DDIRRLSASDLLLAAVGPITAGPLRAAGLAPEYPDRGRMGALVRLVITRLGVDQPAIA
                     TPAGELRVRAGRATLDHTRFDLSAGTLAVLRELAREPGMVVSRERLLAVLPGDSLDPH
                     AAEVAVARLRSGLGNPGVVKTVYRRGYQLVVTPASSTVTAASPTVTPASSTVTPASPR
                     VTPASPTVTPASPTVTPAPVAAASRVVPPHAALEA"
     gene            3027..>3605
                     /locus_tag="PLF56_10245"
     CDS             3027..>3605
                     /locus_tag="PLF56_10245"
                     /inference="COORDINATES: protein motif:HMM:NF014016.2"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_function: GO:0016829 - lyase activity [Evidence IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="CbiX/SirB N-terminal domain-containing protein"
                     /protein_id="HOQ53980.1"
                     /translation="MMHPRPTTLVACSHGTRSAEASALVTALVDAVTDRLPGTPVVEM
                     YVDVQQPELESALPRLEGEAVVVPLFLSGGYHLHHDIHRAAAVHPRAMVSAPLGPDPV
                     LTDLLMQRLVAAGWRAGDAVVLAASASSDPRAVQDVRRAATLLSERVGAEVGIGVVGG
                     AGVQIGEAVAAARRPGRRVAVATYLLMPGFLHG"
BASE COUNT          443 a         1276 c         1284 g          602 t
ORIGIN      
        1 cattggatcg acgactggcg tcccgaagac ctcgagttct ggaacaccac cggggcgaaa
       61 atcgcccggc gcaacctgat ctactcggtc gccagcgagc acatcggctt ctcggtgtgg
      121 acgctgtggt cggtgctggt gctgttcctc ggccctgact acggcttcga cgtcagccag
      181 aagtttctgc tgaccgccgt gccgtccctg ctcggcgccc tggtgcgggt gccgtatacg
      241 ttcgcggtgg ccgtgttcgg cggccgcaac tggacggtga tctcgaccct gctgctgctg
      301 atcccgaccg tcgccatcgc catcgtgctg cagccggggg tgtcgttcac caccctgctg
      361 tgggtgagcg ccctggccgg ggtcggcggg ggcaacttcg cctcgtccat gacgaacatc
      421 aacgccttct tcccggcccg gctgaagggc tgggcgctgg gcgtcaacgc cggcggcggc
      481 aacctggggg tcgcggtggc gcagctcatc ggtctggccg tgctggcgct gaccggcacc
      541 cagtacccgc ggctgatggt cgccatctac attccgctgg tgctgctggt cgccacgctc
      601 gcggcgctca agatggacaa cctcggcgtg gtcagcaacg ccaagggcgc gatgcgcgaa
      661 gtcatcacgc atcgcgagag ctgggtgctg gcactgctct acatcggcac gttcggttcg
      721 ttcatcggct tctcgttcgc cttcggccag gtactgctca cccagtttcc caaggagttc
      781 cccaacccgg ccgccgcggc cgcgctgacc tttctcggcc cgctgctggg ctcgctgatc
      841 cggccggtcg ggggcaagct ggccgaccgc ttcggcggtt cggtggtgac cttctggaac
      901 ttcctggcga tgggtctcgg caccgccctg gtgctgctgg cggctcagct gcactcgctg
      961 gcgttgttcg tgaccggctt catcatgctg ttcgtgttct cgggtctcgg caacggctcc
     1021 acctacaaga tgatcccggc gatcttcacg gccaaggcac agatcgctgt cggcgcgggg
     1081 cagcccatcg agcacgagat ggcccagtcg ctgcggcgct cgggtgcgct gatcggtctc
     1141 gccggtgcga tcggcgccct gggcggattc ggggtcaacc tggccttccg gcagtcgttc
     1201 ctcaccatgg gcaacgccaa cggcgcgtac ctggtgttca tcggcttcta cgtggtctgc
     1261 atggtggtca cctggcagtg cttcatgcgg gctcgcgccg gccggctgga gggggtctga
     1321 ttcagcccgg atccaccgat cttcgcccac tgctcggccc catcggtgga tccgggctca
     1381 ggtgcggccg ggcgcgcggc ctcatcggtg gatccgggct caggtgcggc cgggcgcgcg
     1441 gcctcatgcc gcgtcgcccg gtcgttccgg tggcccgccc cgccagcggc catcaggtgc
     1501 gccgctgccc ggggtcacga cgaggtcctg gggccgacga gcccgcggcc agctttcgca
     1561 caccgcggag cgccggtggt gagagtcttg tgccccgtcc gttacacgac cggagcattc
     1621 ccgacatcgg cctctcacat aatcggacga tgcaccccca ccgaaccagc acgcgttgcc
     1681 cggcagtcac accgccgccg ccgcgcgtcc ggcgacggcg agtgcggcac gaagggggtg
     1741 gggttcatgc tcggtccgat tcttgaaggc gcgcgggtgc tggtcactgc gcaacgccgg
     1801 gcggacgatc tggccgccgc gctgatcagg cgcggagccc aggtcgacat cgcctcgacc
     1861 ctcggcgtgg agacgcatat cgacgaggcc gggttgctgg agcgcacccg ccagctgctc
     1921 taccgcccgc ccgcgatcgt cgtggtgacc accgcgatcg gactgcgggg atggctggag
     1981 accgccgaga cctccgggct gggagcaccg ttcgtcgaac tgttgcgcgc ctcccgggtg
     2041 atcgcacgcg gccccaaggc actcggcgcc ctgcaaggaa tcggagtgac gcccgactgg
     2101 gtggccgaat cggagacgtc ccgagagatc ctcgacctgc tgagcgccga gggtgtcgaa
     2161 gggcaacgga tcgccgtgca gctgcacggt gccggtgatg acggactggt gtccggcctc
     2221 gtccgggcgg gggcggacgt ggtgccgctc accgtgtacc gctggtgttc cccgcacgat
     2281 ccggaggccg tcctggagtc ggtggacctg gcccgcgccg gggcctacga cgcggtcacc
     2341 ttcacctcgg caccaggtgc cgccgcctgg atcgccgccg tcgagagccg gggtgccctg
     2401 gacgacatcc gccggctgag cgcctcggat ctactgctgg ccgcggttgg tccgatcacc
     2461 gcggggccgc tcagggcggc gggactggca cccgaatacc ccgaccgggg tcggatggga
     2521 gccctggtca ggctggtgat cacccggctg ggcgtcgacc agccggccat cgccaccccc
     2581 gccggcgaac tgcgggtgcg ggcgggtcgg gcgacgctgg accacacccg gttcgacctg
     2641 tcggccggca ccctcgccgt gctgcgcgag ctggcccgcg agcccggcat ggtggtgagc
     2701 cgtgagcggc tgctcgccgt gctgcccggc gactccctcg atccgcacgc ggccgaggtc
     2761 gcggtcgcgc ggctgcggtc cggcctcggc aatcccggcg tggtcaagac cgtgtaccgc
     2821 aggggctatc aactggtggt cacgccggca tcgtcgaccg tcacggcggc atcgccgact
     2881 gtcacgccgg catcgtcgac tgtcacgcca gcatcgccga gggtcacccc ggcatcgccg
     2941 actgtcacgc cggcatcgcc gactgttacc ccggcaccgg tcgcggccgc ctcgcgggtt
     3001 gtcccgcccc atgctgccct ggaggcatga tgcacccccg tccgaccacc ctggtcgcct
     3061 gcagccacgg cacccgctcg gccgaggcga gtgccctggt caccgccctc gtcgacgcgg
     3121 tcacggaccg gctgccgggt acgcccgtgg tcgagatgta cgtggacgtg caacagcccg
     3181 agttggagtc ggcgctgccc cggctggaag gtgaggcggt ggtggtgccg ctgttcctgt
     3241 cggggggcta ccacctgcat cacgacatcc atcgcgcggc cgccgttcac ccgcgcgcga
     3301 tggtcagcgc cccgctcggc cccgatcccg tgctcacgga cctgctgatg cagcggctgg
     3361 tggccgccgg gtggcgggcc ggcgacgcgg tggtgttggc ggcgtcggct tcgtccgacc
     3421 cgcgcgcggt gcaggacgtg cgtcgagcgg cgaccctgtt gtccgagcgc gtcggcgccg
     3481 aggtcggcat cggcgtggtc ggcggcgcgg gcgtacagat cggcgaggcg gtggcggcag
     3541 cgcgccggcc cggacgccgg gtcgcagtgg cgacctacct gttgatgccc ggcttcttgc
     3601 acggc
//
LOCUS       DAOSUG010000319         3587 bp    DNA     linear   ENV 10-JUN-2024
DEFINITION  MAG TPA_asm: Micropruina sp. isolate AMR_MDS_2195
            NODE_10664_length_3587_cov_4.585221, whole genome shotgun sequence.
ACCESSION   DAOSUG010000319 DAOSUG010000000
VERSION     DAOSUG010000319.1
DBLINK      BioProject: PRJNA925477
            BioSample: SAMN32864262
            Sequence Read Archive: SRR4236673
KEYWORDS    WGS; ENV; Metagenome Assembled Genome; MAG; Third Party Data; TPA;
            TPA:assembly.
SOURCE      Micropruina sp. (wastewater metagenome)
  ORGANISM  Micropruina sp.
            Bacteria; Bacillati; Actinomycetota; Actinomycetes;
            Propionibacteriales; Nocardioidaceae; Micropruina.
REFERENCE   1  (bases 1 to 3587)
  AUTHORS   Abdulkadir,N., Saraiva,J.P., Zhang,J., Stolte,S., Gillor,O.,
            Harms,H. and Rocha,U.
  TITLE     Genome-centric analyses of 165 metagenomes show that mobile genetic
            elements are crucial for the transmission of antimicrobial
            resistance genes to pathogens in activated sludge and wastewater
  JOURNAL   Microbiol Spectr 12 (3), e0291823 (2024)
   PUBMED   38289113
REFERENCE   2  (bases 1 to 3587)
  AUTHORS   Abdulkadir,N.
  TITLE     Direct Submission
  JOURNAL   Submitted (24-MAR-2023) Environmental Microbiology, Helmholtz
            Center for Environmental Research -UFZ, Permoserstr 15, Leipzig
            04318, Germany
COMMENT     The annotation was added by the NCBI Prokaryotic Genome Annotation
            Pipeline (PGAP). Information about PGAP can be found here:
            https://www.ncbi.nlm.nih.gov/genome/annotation_prok/
            
            ##Genome-Assembly-Data-START##
            Assembly Method        :: SPAdes v. 3.11.1
            Genome Representation  :: Full
            Expected Final Version :: Yes
            Genome Coverage        :: Not Applicable
            Sequencing Technology  :: Illumina MiSeq
            ##Genome-Assembly-Data-END##
            
            ##Genome-Annotation-Data-START##
            Annotation Provider               :: NCBI
            Annotation Date                   :: 05/04/2023 04:57:10
            Annotation Pipeline               :: NCBI Prokaryotic Genome
                                                 Annotation Pipeline (PGAP)
            Annotation Method                 :: Best-placed reference protein
                                                 set; GeneMarkS-2+
            Annotation Software revision      :: 6.5
            Features Annotated                :: Gene; CDS; rRNA; tRNA; ncRNA
            Genes (total)                     :: 2,776
            CDSs (total)                      :: 2,750
            Genes (coding)                    :: 2,714
            CDSs (with protein)               :: 2,714
            Genes (RNA)                       :: 26
            tRNAs                             :: 24
            ncRNAs                            :: 2
            Pseudo Genes (total)              :: 36
            CDSs (without protein)            :: 36
            Pseudo Genes (ambiguous residues) :: 0 of 36
            Pseudo Genes (frameshifted)       :: 2 of 36
            Pseudo Genes (incomplete)         :: 32 of 36
            Pseudo Genes (internal stop)      :: 2 of 36
            ##Genome-Annotation-Data-END##
FEATURES             Location/Qualifiers
     source          1..3587
                     /organism="Micropruina sp."
                     /mol_type="genomic DNA"
                     /submitter_seqid="NODE_10664_length_3587_cov_4.585221"
                     /isolate="AMR_MDS_2195"
                     /isolation_source="Activated sludge of municipal
                     wastewater treatment plants"
                     /db_xref="taxon:2737536"
                     /environmental_sample
                     /geo_loc_name="Canada"
                     /lat_lon="49.25 N 123.1 W"
                     /collection_date="2015-06-16"
                     /metagenome_source="wastewater metagenome"
                     /note="metagenomic"
     gene            complement(<1..221)
                     /locus_tag="PLF56_10250"
     CDS             complement(<1..221)
                     /locus_tag="PLF56_10250"
                     /inference="COORDINATES: ab initio
                     prediction:GeneMarkS-2+"
                     /note="Derived by automated computational analysis using
                     gene prediction method: GeneMarkS-2+."
                     /codon_start=1
                     /transl_table=11
                     /product="hypothetical protein"
                     /protein_id="HOQ53981.1"
                     /translation="MSRQSWIGPVTLAGWGVQVVPLKPEHAPGLAAAAADGELWRLRI
                     TSVPAPGDEPAYIQTALTTDDRVAFAVLDG"
     gene            complement(218..1489)
                     /locus_tag="PLF56_10255"
     CDS             complement(218..1489)
                     /locus_tag="PLF56_10255"
                     /EC_number="2.3.3.16"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_020580257.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_function: GO:0046912 - acyltransferase activity, acyl
                     groups converted into alkyl on transfer [Evidence IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="citrate synthase"
                     /protein_id="HOQ53982.1"
                     /translation="MAESLRISAGEVSADLPITEGAVRATKLSAIPTDRGGLSSYDPG
                     FVNTAACRSAITYIDGEQGILEHRGYPIEELAEKSSFLEVAYLLSHSELPNQAQLDEW
                     THQINRHRLVHENVYDIIKSFRYDAHPMAKLMASVVTQMTFYPSSRRIADPEARALNS
                     FRLLGKMPTLAAATFRHSIGRHPVYPSDELSYVENFLAMLFQSQQRVWRADPRLVRAL
                     EVLFILHADHEQNCSTTAVRTVASSGNDVYTSAAAGIGALFGPLHGGANEAVLKMLRE
                     IGTLDRVPDFIEGVKNGKGRLMGFGHRVYKNYDPRAKVIKQAVDDVFEVRGMNPLLDI
                     AVELEKIALNDEYFVSRKLYPNVDFYSGLIYEALNFPTEMFTVLFAVPRTAGWVAHWT
                     EQLTDPDQRIIRPKQIYTGYERRPYVPIEER"
     gene            1601..1987
                     /locus_tag="PLF56_10260"
     CDS             1601..1987
                     /locus_tag="PLF56_10260"
                     /inference="COORDINATES: protein motif:HMM:NF014019.2"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="YbjQ family protein"
                     /protein_id="HOQ53983.1"
                     /translation="MLIVTTNDIAGYRIEAVFGEVMGLTVRSANIGANFVASFRAIGG
                     GEVNEYTQLVYESRQQVMQRMWDQAQQRGANAIIGCRFDTGEIAAQFSEVCCYGTAVA
                     VVPIAEGEPGATPQSIADAQARGTMR"
     gene            1987..>3587
                     /locus_tag="PLF56_10265"
     CDS             1987..>3587
                     /locus_tag="PLF56_10265"
                     /inference="COORDINATES: protein motif:HMM:NF037018.2"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_function: GO:0003824 - catalytic activity [Evidence
                     IEA];
                     GO_process: GO:0005975 - carbohydrate metabolic process
                     [Evidence IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="alpha-amylase family glycosyl hydrolase"
                     /protein_id="HOQ53984.1"
                     /translation="MRTLTGAPDLTLAPGHWVTRDLVAWPVDAVPYGIDPHGLDWRLH
                     WSAEGGIDPDAREPVSWNTGELTLDPDGLPEAVLQRRPQLRGCLALRVAPDAAAHAVQ
                     ALRGQVLLSVHQPSGKLVTAGSLQIPGVLDDLYAAAADARLGVTWREGSPTLRLWAPT
                     ARTVRLMLWCPLDDLHAPGRPFEATREPDGCWSVGGTPEWRGARYRWEVTVFAPSGRR
                     MVTNQVTDPYSVSLTVNSTHSVIVDLDDAALRPVQWCGAPQPRLAHPVDQVSYELHVR
                     DFSIHDKSVPETVRGSFLAFAHDSLGTRHLRRLAQAGLNTVQLLPVFDNATVEESRDR
                     QRGPAKADLRAQPPDSPNQQRLIRLTVVGNPYNWGYDPWHYQAVEGSFCSSLASAEGS
                     GRVHEFRTMIGALHAMGLRVVLDQVFNHTASFGQNTKSVLDRTVPGYYHRLDPLGFTE
                     HSTCCPNVATEHAMAQKLMVDSVVLWARDYKVDGFRFDLMGHHTRDNMLAVRAALDAL
                     TPETDGVDGRQVTIYGEGWNFGEVA"
BASE COUNT          582 a         1201 c         1216 g          588 t
ORIGIN      
        1 ccgtccagca ccgcgaacgc cacccggtcg tcggtggtca gcgccgtctg aatgtaggcc
       61 ggttcgtcgc ccggggcggg cacgctggtg atgcgcagcc tccacagttc gccatcggct
      121 gcggccgcgg ccaggcccgg ggcgtgttcg ggcttcagcg gcaccacctg caccccccac
      181 ccggccaggg tgacgggacc gatccaggac tgtcggctca acgctcctcg atcggcacgt
      241 acggccgtcg ctcgtagccg gtgtagatct gcttgggacg aatgatgcgc tgatcggggt
      301 cggtgagttg ctcggtccag tgcgcgaccc agcccgcggt gcgcggcacc gcgaacaaca
      361 cggtgaacat ttcggtggga aagttcaacg cctcataaat caggcccgaa tagaaatcga
      421 cgttggggta gagcttgcgt gagacgaagt attcgtcgtt gagcgcgatc ttttcgagtt
      481 cgacggcgat gtcgagcagc ggattcatgc cgcgcacctc gaacacgtca tcgactgcct
      541 gcttgatcac cttggcccgc ggatcgtagt tcttgtacac gcggtggccg aagcccatca
      601 accggccctt gccgttcttg acgccctcga tgaaatcggg cacccgatcg agcgtgccga
      661 tctcgcgcag catcttcagc accgcctcgt tggcgccgcc gtgcagcgga ccgaacagcg
      721 ccccgatacc tgccgccgcc gaggtgtaca cgtcgttgcc ggacgaggca acggtgcgca
      781 ccgccgtggt cgaacagttc tgctcatgat cggcatgcag aatgaacagc acctcgagcg
      841 cccggacgag ccgtggatcg gcccgccaca cccgctgctg ggattggaac agcatggcca
      901 ggaagttctc gacataactg agttcgtcac tggggtagac cgggtgccgg ccgatcgagt
      961 ggcggaacgt ggccgccgcc agcgtcggca tcttgcccag cagccggaac gagttcagcg
     1021 cacgcgcctc ggggtcggcg atgcgacgag acgacgggta gaacgtcatc tgcgtcacca
     1081 cggaagccat cagcttggcc atcgggtgcg cgtcatagcg aaacgacttg atgatgtcgt
     1141 acacgttctc gtgcaccagc cggtgccggt tgatctggtg ggtccattcg tccagctgcg
     1201 cctgattggg caactcggaa tggctcaaca gatacgccac ttcgagaaag gacgacttct
     1261 cggcaagctc ttcgatcggg tagccccgat gttcgagaat gccctgctcg ccgtcgatgt
     1321 aggtgatggc gctacgacac gcggcggtgt tcacgaaccc cgggtcatac gacgacaacc
     1381 cgccgcggtc ggtcggaatg gcagagagct tggtcgcgcg gacggccccc tcggtgatcg
     1441 gcaggtctgc ggatacctcg cctgcactga ttcgcaacga ttcggccatg cctcacccta
     1501 ttcagcccat tcggcggccc gccggagaag ccgcaggctg agaccggcga cacccccgcg
     1561 ggcccgctgc tctacgctgc cgcgccctta cgatggggcc atgctgatcg tcaccaccaa
     1621 cgacattgcc gggtaccgca tcgaagccgt gttcggcgaa gtgatgggcc tcaccgttcg
     1681 gtccgcgaat atcggggcga acttcgtggc cagctttcgg gcgatcggcg gcggcgaggt
     1741 caacgaatac acccagttgg tctacgagtc gcggcagcag gtgatgcagc gcatgtggga
     1801 ccaggcccag cagcgaggtg ccaacgcgat catcggctgc cggttcgaca ccggcgagat
     1861 cgccgcgcag ttctctgagg tgtgctgtta cggcactgcc gtcgcggtgg ttccgatcgc
     1921 ggaaggcgag cccggcgcca ccccccagtc gatcgccgac gcccaggcgc ggggcaccat
     1981 gcgctgatgc gcaccctgac cggtgcgccg gatctcaccc tcgcacccgg ccactgggtc
     2041 acccgcgacc tggtggcgtg gccggtggac gccgtaccgt acggcatcga cccgcacggg
     2101 ctggactggc gcctgcattg gtcggccgag ggcggcatcg acccggatgc ccgtgaaccg
     2161 gtgagctgga acaccggtga gctgactctc gatccggacg gactgcccga ggcggtgctg
     2221 caacgtcgcc cgcagttgcg tggctgcctg gccctacggg tggccccgga cgccgcggcg
     2281 cacgccgtgc aggcgttgcg gggccaggtg ctgctgagcg tgcaccagcc gtccggcaag
     2341 ctggtcaccg caggctccct gcagattccc ggcgtgctgg acgacctgta cgcagcggcc
     2401 gccgacgccc ggctcggcgt gacctggcgt gagggttcgc cgaccctgcg gctgtgggcg
     2461 ccgaccgcgc gcaccgtgcg gctgatgctg tggtgcccct tggacgacct gcacgcgccg
     2521 gggcgtccgt tcgaggcgac ccgcgaaccg gacggctgct ggtcggtggg gggcacgccg
     2581 gagtggcgcg gcgcccgcta ccggtgggag gtgacggtgt tcgccccgtc cggacggcgc
     2641 atggtgacca accaggtgac cgatccctat tcggtctcgc tgacggtgaa ctcgacccac
     2701 tcggtgatcg tcgacctcga cgatgccgcg ctgcggcccg tccagtggtg cggagccccg
     2761 cagccccggc tggcgcaccc ggtcgaccag gtcagttacg aacttcatgt gcgggacttc
     2821 tcgatccacg acaagtctgt tcccgagacc gtccggggca gctttctcgc gttcgcccac
     2881 gacagtctgg gcacccggca cctgcgccgg ctggcccagg cggggttgaa cacggtccag
     2941 ttgttgccgg tgttcgacaa cgcaacggtt gaagagtcgc gcgatcgtca acgggggccg
     3001 gccaaggccg atctgcgggc gcagccaccc gacagtccca accagcagcg gctgatccgg
     3061 ctgaccgtgg tcggcaaccc gtacaactgg ggctacgacc cgtggcacta ccaggcggtc
     3121 gagggatcgt tctgcagcag cctcgccagc gccgaggggt ccggccgggt gcacgagttc
     3181 cgcaccatga tcggggccct gcacgcgatg gggctgcggg tggtgctcga ccaggtgttc
     3241 aaccacaccg cgagcttcgg gcagaacacc aagtcggtgc tcgatcgcac ggtgccgggc
     3301 tactaccacc ggctcgaccc gctgggtttc accgagcact ccacctgctg ccccaacgtg
     3361 gcgaccgaac atgcgatggc gcagaaactc atggtcgact cggtggtgtt gtgggcccgc
     3421 gactacaagg tcgacgggtt ccggttcgac ctgatggggc accacacgcg cgacaacatg
     3481 ctggccgtgc gcgccgcact cgatgcgctg acccccgaga ccgatggggt ggacgggcgg
     3541 caggtcacga tctacggcga gggctggaac ttcggcgagg tggccca
//
LOCUS       DAOSUG010000320         3584 bp    DNA     linear   ENV 10-JUN-2024
DEFINITION  MAG TPA_asm: Micropruina sp. isolate AMR_MDS_2195
            NODE_10673_length_3584_cov_4.373477, whole genome shotgun sequence.
ACCESSION   DAOSUG010000320 DAOSUG010000000
VERSION     DAOSUG010000320.1
DBLINK      BioProject: PRJNA925477
            BioSample: SAMN32864262
            Sequence Read Archive: SRR4236673
KEYWORDS    WGS; ENV; Metagenome Assembled Genome; MAG; Third Party Data; TPA;
            TPA:assembly.
SOURCE      Micropruina sp. (wastewater metagenome)
  ORGANISM  Micropruina sp.
            Bacteria; Bacillati; Actinomycetota; Actinomycetes;
            Propionibacteriales; Nocardioidaceae; Micropruina.
REFERENCE   1  (bases 1 to 3584)
  AUTHORS   Abdulkadir,N., Saraiva,J.P., Zhang,J., Stolte,S., Gillor,O.,
            Harms,H. and Rocha,U.
  TITLE     Genome-centric analyses of 165 metagenomes show that mobile genetic
            elements are crucial for the transmission of antimicrobial
            resistance genes to pathogens in activated sludge and wastewater
  JOURNAL   Microbiol Spectr 12 (3), e0291823 (2024)
   PUBMED   38289113
REFERENCE   2  (bases 1 to 3584)
  AUTHORS   Abdulkadir,N.
  TITLE     Direct Submission
  JOURNAL   Submitted (24-MAR-2023) Environmental Microbiology, Helmholtz
            Center for Environmental Research -UFZ, Permoserstr 15, Leipzig
            04318, Germany
COMMENT     The annotation was added by the NCBI Prokaryotic Genome Annotation
            Pipeline (PGAP). Information about PGAP can be found here:
            https://www.ncbi.nlm.nih.gov/genome/annotation_prok/
            
            ##Genome-Assembly-Data-START##
            Assembly Method        :: SPAdes v. 3.11.1
            Genome Representation  :: Full
            Expected Final Version :: Yes
            Genome Coverage        :: Not Applicable
            Sequencing Technology  :: Illumina MiSeq
            ##Genome-Assembly-Data-END##
            
            ##Genome-Annotation-Data-START##
            Annotation Provider               :: NCBI
            Annotation Date                   :: 05/04/2023 04:57:10
            Annotation Pipeline               :: NCBI Prokaryotic Genome
                                                 Annotation Pipeline (PGAP)
            Annotation Method                 :: Best-placed reference protein
                                                 set; GeneMarkS-2+
            Annotation Software revision      :: 6.5
            Features Annotated                :: Gene; CDS; rRNA; tRNA; ncRNA
            Genes (total)                     :: 2,776
            CDSs (total)                      :: 2,750
            Genes (coding)                    :: 2,714
            CDSs (with protein)               :: 2,714
            Genes (RNA)                       :: 26
            tRNAs                             :: 24
            ncRNAs                            :: 2
            Pseudo Genes (total)              :: 36
            CDSs (without protein)            :: 36
            Pseudo Genes (ambiguous residues) :: 0 of 36
            Pseudo Genes (frameshifted)       :: 2 of 36
            Pseudo Genes (incomplete)         :: 32 of 36
            Pseudo Genes (internal stop)      :: 2 of 36
            ##Genome-Annotation-Data-END##
FEATURES             Location/Qualifiers
     source          1..3584
                     /organism="Micropruina sp."
                     /mol_type="genomic DNA"
                     /submitter_seqid="NODE_10673_length_3584_cov_4.373477"
                     /isolate="AMR_MDS_2195"
                     /isolation_source="Activated sludge of municipal
                     wastewater treatment plants"
                     /db_xref="taxon:2737536"
                     /environmental_sample
                     /geo_loc_name="Canada"
                     /lat_lon="49.25 N 123.1 W"
                     /collection_date="2015-06-16"
                     /metagenome_source="wastewater metagenome"
                     /note="metagenomic"
     gene            complement(151..945)
                     /locus_tag="PLF56_10270"
     CDS             complement(151..945)
                     /locus_tag="PLF56_10270"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_013861747.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="AAC(3) family N-acetyltransferase"
                     /protein_id="HOQ53985.1"
                     /translation="MRPTTPADPITAERLVRDLHALGLQPGATVMVHTSLRSLGWVVG
                     GCQAVLEALSTAAGADGTLIMPTQSWQLCDPALLNEAPEKWWPTIRENLPLYDPATTP
                     SQTMGAVAELFRTTPRALRSAHPHRSIAAAGPQAETITASHPLEAPAGEESPLRALVD
                     LDGQVLLLGVTAAKLTLLHLAEHRASYPGKTTIPNGVAMMVDGRRQWVTWNELDVHDD
                     DFVDVVEAFAADTGLVSTGLVGEATAQLMPARPLVEFATNWFSTHR"
     gene            complement(950..1945)
                     /locus_tag="PLF56_10275"
     CDS             complement(950..1945)
                     /locus_tag="PLF56_10275"
                     /inference="COORDINATES: protein motif:HMM:NF013567.2"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_function: GO:0016491 - oxidoreductase activity
                     [Evidence IEA];
                     GO_function: GO:0016651 - oxidoreductase activity, acting
                     on NAD(P)H [Evidence IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="Gfo/Idh/MocA family oxidoreductase"
                     /protein_id="HOQ53986.1"
                     /translation="MDQVRWGIIGVGDVTEVKSGPGFQQADGSDLVAVMRRNGELARD
                     YAQRHGVARAHDDADALIADPEVDAVYIATPPDSHSDYAIRVAQAGKPVYIEKPMGRT
                     GAECRRVLDVAERAGVPVFVAFYRRTMPRFELARELIATELGEVGAVLVRCQQRAHPL
                     IDGEVPWRLNPAVAGGGLFVDLGSHTLDWLDHVFGPIRSVSGHAGRPLAGRGTVETVV
                     SASFAFDGVVGSGLWDFDASEPLDLIEVVGTRGTLRMSSFTAEPLELIRGAERTLIEA
                     PYPVTVQLPMIQQITDVLLGRAEVAVSTGATALRTAEVVDTVLADYRAEHDLSLG"
     gene            2296..3294
                     /gene="dhaK"
                     /locus_tag="PLF56_10280"
     CDS             2296..3294
                     /gene="dhaK"
                     /locus_tag="PLF56_10280"
                     /EC_number="2.7.1.121"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_013864710.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_function: GO:0004371 - glycerone kinase activity
                     [Evidence IEA];
                     GO_process: GO:0006071 - glycerol metabolic process
                     [Evidence IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="dihydroxyacetone kinase subunit DhaK"
                     /protein_id="HOQ53987.1"
                     /translation="MKKLINDPSDVVPETLKGFAAANAELITVHFDPDYVVRADAPKQ
                     GKVGLVSGGGSGHEPLHAGFVGLGMLDAAVPGAVFTSPTPDPILEATKAVDGGAGVLH
                     IVKNYTGDVLNFETAAELADLEGINVKAVVVNDDVAVEDSTWTAGRRGVAGTVLVEKI
                     AGAAAERGDDLDAVTAVAEKVIGQVRSMGVALTACTVPHAGKPSFDLGEDEIEMGVGI
                     HGEPGRHRVPMASANAITDQLVDAILDDLKLRGGQVLLFVNGMGGTPESELYIVYGHA
                     RERLEAAGLEVTRSLVGNYITSLEMQGCSITVLELDDELTQLWDAPVNTVALRRGI"
     gene            3295..>3584
                     /locus_tag="PLF56_10285"
     CDS             3295..>3584
                     /locus_tag="PLF56_10285"
                     /inference="COORDINATES: protein motif:HMM:NF014754.2"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_function: GO:0004371 - glycerone kinase activity
                     [Evidence IEA];
                     GO_process: GO:0006071 - glycerol metabolic process
                     [Evidence IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="DAK2 domain-containing protein"
                     /protein_id="HOQ53988.1"
                     /translation="MTNLDVEWAKDWMRRASANLTEHRMELIDLDRAIGDGDHGENMA
                     RGFTFVVERIDANPPEDVAGVFKLVAQTLMAKVGGASGPLYGTAFMRAAKAA"
BASE COUNT          585 a         1189 c         1264 g          546 t
ORIGIN      
        1 caaggagaac gccaccgccg acaacttctg ggatccggtc ggctaccagg acgcgtacaa
       61 ggcgatctgg ggcaagtgac cccgtccgaa ccagcgtgag ccaaggtccg tcccgcaatc
      121 ggggcggacc ttggccggtt ccgcgggcga tcagcggtgg gtgctgaacc agttcgtcgc
      181 aaactcgacc agcggacggg cgggcatcag ctgcgccgtc gcctcgccca cgaggccggt
      241 gctcaccagg ccggtgtcgg cggcgaaggc ctcgacgacg tcgacgaagt cgtcatcgtg
      301 aacgtcgagc tcgttccagg tgacccactg tcggcgcccg tcgaccatca tggcgacccc
      361 gttgggaatg gtggtcttgc ccgggtagga cgctcggtgc tcggcgaggt gaagaagcgt
      421 gagcttcgca gccgtcacgc cgagcagtag aacctggccg tcgaggtcga cgagcgcgcg
      481 cagcggcgac tcctccccgg ctggggcttc gagggggtgg gaggcggtga tcgtctcggc
      541 ctgggggccg gcggcagcga tggaccggtg cgggtgtgca ctgcggagtg ctcgcggtgt
      601 ggtccggaac agttccgcga cggcgcccat ggtctggctg ggtgtggtgg ctgggtcgta
      661 gagaggcaag ttctcgcgga tggtcggcca ccacttctca ggagcctcgt tgagcagggc
      721 cgggtcgcag agctgccacg actgggtcgg catgatgagg gtgccgtccg ctccggctgc
      781 agtgctgagg gcttcgagaa ctgcctggca cccgccgacg acccatccga gtgagcgtag
      841 ggacgtgtga accatgacgg tcgcgccggg ctgcaggccc agcgcgtgca ggtccctgac
      901 gaggcgttcc gccgtgatcg ggtcggctgg ggtcgtcggt ctcatgacat cagccgagcg
      961 agaggtcgtg ctcggcccgg tagtcggcca gcacggtgtc gaccacctcg gcggtgcgca
     1021 gggcggtggc tccggtgctg acggcgactt cggcgcgtcc gagcagcacg tcggtgatct
     1081 gctggatcat cggcagttgc acggtgaccg ggtagggcgc ctcgatcagg gtgcgttcgg
     1141 cgccgcggat cagctcgagt ggctctgcgg tgaacgacga catgcgcagg gtgcccctgg
     1201 tgccgaccac ctcgatcagg tcgagtggct cggaggcgtc gaagtcccac agacccgaac
     1261 ccaccacccc gtcgaaggcg aatgacgccg acaccaccgt ctcgacggtg ccgcggccgg
     1321 ccaggggacg tccggcatgc ccgctgacgc tgcggatcgg gccgaagacg tgatcgagcc
     1381 agtcgagggt gtgcgagccg agatcgacga agagcccccc gccggcgacg gcggggttca
     1441 accgccacgg cacctcgcca tcgatcagcg ggtgggcgcg ctgctggcac cggaccagca
     1501 ccgcgccgac ctcgccgagt tcggtggcga tcagttcgcg ggccagctcg aagcgcggca
     1561 tcgtccggcg atagaacgcg acgaacaccg ggacgcctgc ccgctcggcc acatcgagga
     1621 cgcgccggca ctcggcaccg gtgcgtccca tcggcttctc gatgtagacc ggcttgcccg
     1681 cctgcgcgac acgaatggcg tagtcgctgt gcgaatcggg cggcgtggcg atgtagacgg
     1741 cgtcgacctc ggggtcggcg atcagggcgt ccgcatcgtc gtgggccctt gccacaccgt
     1801 gccgctgcgc gtagtcgcgc gccagttcgc cgttgcgccg catgacggcc accaggtccg
     1861 aaccgtcggc ctgctgaaag ccaggcccgc tcttgacctc ggtgacgtca cccacgccga
     1921 tgattcccca tcgcacctga tccatgccga ccagacaacc acatgcacga cggaaccccg
     1981 gcaccgctcg cgaaccggat cagggggaac cggacggtgg ccgagacacc atcagcgagc
     2041 tcttccctcc ctcgacggcc ccgtcgtccg cgatcccccc ggatcgttga cgtcagccgc
     2101 gtcgcgaagg ccggcgggcg ttccgcagcc tcggcccgat ccgggaaacg tccgatagtt
     2161 gactgaacat gtgtagacaa gttgaatggg gccacttccc tagcgaggta actttcggca
     2221 gagtggactc gcgccgatcg gtgcgtccgc ggccgggggt gtccgcggtc ttgagccaca
     2281 ggagtgtgtg tttcgatgaa gaaactcatc aacgatccgt ccgatgtcgt acccgaaacc
     2341 ctgaaggggt tcgccgccgc caacgccgaa ctgatcaccg tccatttcga tcccgactac
     2401 gtggtgcgcg ccgacgcgcc caagcagggc aaggtcggcc tggtctcggg cggcggctcg
     2461 ggccacgagc cgctgcacgc cggcttcgtc ggcctcggca tgctggacgc ggccgtcccg
     2521 ggcgcggtct tcacctcgcc gacgcccgac ccgatcctcg aggccaccaa ggccgtcgac
     2581 ggcggcgccg gggtgctgca catcgtgaag aactacaccg gcgacgtgct caacttcgag
     2641 accgccgccg aactggccga cctggagggc atcaacgtca aggccgtcgt ggtcaatgac
     2701 gacgtggccg tcgaggattc cacctggacc gccggacgcc gcggcgtggc cggcaccgtg
     2761 ctggtggaga agatcgccgg cgccgccgcc gagcgtggcg acgacctgga cgcggtgacc
     2821 gccgtcgccg agaaggtgat cggacaggtg cgttcgatgg gcgtggcgct caccgcctgc
     2881 accgtgccgc acgccggcaa gccgtccttc gacctcggtg aggacgagat cgagatgggc
     2941 gtcggcattc acggtgagcc ggggcggcat cgggtgccga tggcgagcgc caacgcgatc
     3001 accgaccaac tcgtcgacgc gatcctcgac gacctgaagt tgcgcggtgg tcaggtgctg
     3061 ctcttcgtca acggcatggg cggcacgccc gaatccgagc tgtacatcgt ctacgggcat
     3121 gcccgcgagc gtttggaggc ggcggggctc gaggtgaccc ggtcgttggt gggcaactac
     3181 atcacctcgc tcgaaatgca gggttgcagc atcaccgtcc tcgaactcga tgacgaactc
     3241 acacaactgt gggacgctcc ggtcaacact gtggcgctca gaaggggcat ctgaatgacg
     3301 aatctcgacg tcgagtgggc caaggactgg atgcgccgag cctccgccaa cctcaccgaa
     3361 caccgcatgg aactgatcga tctcgaccgt gcgatcggcg acggtgacca tggcgagaac
     3421 atggcgcgcg gcttcacgtt cgtggtcgaa cgcatcgacg cgaacccgcc cgaggacgtg
     3481 gccggggtgt tcaagctggt cgcccagacc ctgatggcca aggtcggcgg cgcgtccggc
     3541 ccgctgtacg gcaccgcgtt catgcgggcc gccaaggccg ccga
//
LOCUS       DAOSUG010000321         3581 bp    DNA     linear   ENV 10-JUN-2024
DEFINITION  MAG TPA_asm: Micropruina sp. isolate AMR_MDS_2195
            NODE_10686_length_3581_cov_4.368406, whole genome shotgun sequence.
ACCESSION   DAOSUG010000321 DAOSUG010000000
VERSION     DAOSUG010000321.1
DBLINK      BioProject: PRJNA925477
            BioSample: SAMN32864262
            Sequence Read Archive: SRR4236673
KEYWORDS    WGS; ENV; Metagenome Assembled Genome; MAG; Third Party Data; TPA;
            TPA:assembly.
SOURCE      Micropruina sp. (wastewater metagenome)
  ORGANISM  Micropruina sp.
            Bacteria; Bacillati; Actinomycetota; Actinomycetes;
            Propionibacteriales; Nocardioidaceae; Micropruina.
REFERENCE   1  (bases 1 to 3581)
  AUTHORS   Abdulkadir,N., Saraiva,J.P., Zhang,J., Stolte,S., Gillor,O.,
            Harms,H. and Rocha,U.
  TITLE     Genome-centric analyses of 165 metagenomes show that mobile genetic
            elements are crucial for the transmission of antimicrobial
            resistance genes to pathogens in activated sludge and wastewater
  JOURNAL   Microbiol Spectr 12 (3), e0291823 (2024)
   PUBMED   38289113
REFERENCE   2  (bases 1 to 3581)
  AUTHORS   Abdulkadir,N.
  TITLE     Direct Submission
  JOURNAL   Submitted (24-MAR-2023) Environmental Microbiology, Helmholtz
            Center for Environmental Research -UFZ, Permoserstr 15, Leipzig
            04318, Germany
COMMENT     The annotation was added by the NCBI Prokaryotic Genome Annotation
            Pipeline (PGAP). Information about PGAP can be found here:
            https://www.ncbi.nlm.nih.gov/genome/annotation_prok/
            
            ##Genome-Assembly-Data-START##
            Assembly Method        :: SPAdes v. 3.11.1
            Genome Representation  :: Full
            Expected Final Version :: Yes
            Genome Coverage        :: Not Applicable
            Sequencing Technology  :: Illumina MiSeq
            ##Genome-Assembly-Data-END##
            
            ##Genome-Annotation-Data-START##
            Annotation Provider               :: NCBI
            Annotation Date                   :: 05/04/2023 04:57:10
            Annotation Pipeline               :: NCBI Prokaryotic Genome
                                                 Annotation Pipeline (PGAP)
            Annotation Method                 :: Best-placed reference protein
                                                 set; GeneMarkS-2+
            Annotation Software revision      :: 6.5
            Features Annotated                :: Gene; CDS; rRNA; tRNA; ncRNA
            Genes (total)                     :: 2,776
            CDSs (total)                      :: 2,750
            Genes (coding)                    :: 2,714
            CDSs (with protein)               :: 2,714
            Genes (RNA)                       :: 26
            tRNAs                             :: 24
            ncRNAs                            :: 2
            Pseudo Genes (total)              :: 36
            CDSs (without protein)            :: 36
            Pseudo Genes (ambiguous residues) :: 0 of 36
            Pseudo Genes (frameshifted)       :: 2 of 36
            Pseudo Genes (incomplete)         :: 32 of 36
            Pseudo Genes (internal stop)      :: 2 of 36
            ##Genome-Annotation-Data-END##
FEATURES             Location/Qualifiers
     source          1..3581
                     /organism="Micropruina sp."
                     /mol_type="genomic DNA"
                     /submitter_seqid="NODE_10686_length_3581_cov_4.368406"
                     /isolate="AMR_MDS_2195"
                     /isolation_source="Activated sludge of municipal
                     wastewater treatment plants"
                     /db_xref="taxon:2737536"
                     /environmental_sample
                     /geo_loc_name="Canada"
                     /lat_lon="49.25 N 123.1 W"
                     /collection_date="2015-06-16"
                     /metagenome_source="wastewater metagenome"
                     /note="metagenomic"
     gene            complement(<1..737)
                     /locus_tag="PLF56_10290"
     CDS             complement(<1..737)
                     /locus_tag="PLF56_10290"
                     /inference="COORDINATES: ab initio
                     prediction:GeneMarkS-2+"
                     /note="Derived by automated computational analysis using
                     gene prediction method: GeneMarkS-2+."
                     /codon_start=1
                     /transl_table=11
                     /product="hypothetical protein"
                     /protein_id="HOQ53989.1"
                     /translation="MTRDLDDLIRQSLAEHAPAESADPTLGETVVRKGRRVRRTRQAA
                     VAAVALVAVAAGAIGVRTVVTSQPTIATPAPLGPGTTSGPTTSGPTTSGPAPSASASV
                     SPSSTPSATGSSTAHAPIVKDITGMGAENDFGGPTSSFASPTGNLWCNISASGAGCQG
                     QKFNAGAQATVDCEGSRVLGDAVVIEGTGKATWQCTTEIFAFPFLDKNKAFTTWWDPA
                     IGDKRSVRTDSGNVLATLRYGNTLTAG"
     gene            complement(727..1245)
                     /locus_tag="PLF56_10295"
     CDS             complement(727..1245)
                     /locus_tag="PLF56_10295"
                     /inference="COORDINATES: protein motif:HMM:TIGR02983.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_function: GO:0003677 - DNA binding [Evidence IEA];
                     GO_function: GO:0003700 - DNA-binding transcription factor
                     activity [Evidence IEA];
                     GO_function: GO:0016987 - sigma factor activity [Evidence
                     IEA];
                     GO_process: GO:0006352 - DNA-templated transcription
                     initiation [Evidence IEA];
                     GO_process: GO:0006355 - regulation of DNA-templated
                     transcription [Evidence IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="SigE family RNA polymerase sigma factor"
                     /protein_id="HOQ53990.1"
                     /translation="MAITPTQQVLGQDRGFARFVAGEQVALRRYAPALTGNGPDADDL
                     LQATLVKLYLVWDRLSDRDHLGGYARTTMTRTHVSLWRSWGRREVAVAAVPETSAPPT
                     SDAVERDLIWRGLSRLGRRQRAIVVLRYYEDLDLNTIAKELGISVGTVKSQLSRALGN
                     LRTCLEGANRDS"
     gene            1423..2628
                     /locus_tag="PLF56_10300"
     CDS             1423..2628
                     /locus_tag="PLF56_10300"
                     /EC_number="3.5.3.6"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_020390594.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_component: GO:0005737 - cytoplasm [Evidence IEA];
                     GO_function: GO:0016990 - arginine deiminase activity
                     [Evidence IEA];
                     GO_process: GO:0019546 - arginine deiminase pathway
                     [Evidence IEA];
                     GO_process: GO:0019547 - arginine catabolic process to
                     ornithine [Evidence IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="arginine deiminase"
                     /protein_id="HOQ53991.1"
                     /translation="MTELPAAGVRSEVGRLRTVMLHRPGNELRRLTPRNNDALLFDGL
                     PWVDRAQAEHDAFADVLRSRGVEVLYLSELLIATLADPDARQVAIAHTMTDLRLGDTL
                     RRYLSDLLGDQGPDELAEILMAGLRNDEVASDTGSLVAALHAPDDFLIDPLPNLLFTR
                     DSSVWVGDRATVTALAMPARRRETQLTELIYQFHPRFAATERIHGWDKELLEGGDVLA
                     LAEGVLAVGVGERTRPAGAERLARTAFDEGLAHTVLAVPIDQDRATMHLDTICTMVDA
                     DAVVMYPNVAHRLTAVTIKPDEDGDLVIEPPEPFVVAAARAMGIDQLRLIDTGLDPVT
                     AEREQWDDGNNTLAIEPRVAIAYERNVETNDRLAAAGIEVIPIAGSELGSGRGGPRCM
                     SCPISRDAL"
     gene            complement(2962..>3581)
                     /locus_tag="PLF56_10305"
     CDS             complement(2962..>3581)
                     /locus_tag="PLF56_10305"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_014848006.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_function: GO:0003824 - catalytic activity [Evidence
                     IEA]"
                     /codon_start=3
                     /transl_table=11
                     /product="aminotransferase class IV"
                     /protein_id="HOQ53992.1"
                     /translation="REYTFCVIASPVGPYYPDPVTLWATPNYTRAAAGGTGAAKCGGN
                     YAASLAAADEAAEHGCQQVIWLDGAEHRWVEECGTMNIMFVTAEGDLVTPALTGTILD
                     GVTRDSILTLAPEHGLRPVQRQVGIDEVFDGITSGAISEVFACGTAAVVTPVVGFAAP
                     GVNGTDRRFTVGDGTPGALSLAIRAHLLDIQYGRVPDAHGWLRRV"
BASE COUNT          536 a         1255 c         1230 g          560 t
ORIGIN      
        1 tcgccggcgg tgagcgtgtt gccgtaccgc agggtggcca gcacattgcc actgtcggtt
       61 cgcaccgatc gcttgtcacc gatcgcgggg tcccaccagg tggtgaaggc tttgttcttg
      121 tcgaggaagg gaaaggcgaa gatctcggtg gtgcactgcc aggtggcctt gccggtcccc
      181 tcgatcacca cggcatcgcc gagtacccgg ctgccctcgc agtcgaccgt ggcctgcgcg
      241 ccggcgttga acttctggcc ctggcagccg gcgccgctgg ccgagatgtt gcaccacagg
      301 ttgccggtgg gtgaggcgaa cgacgacgtc ggacccccga agtcgttttc tgcgcccatc
      361 ccggtgatgt ccttgacgat cggggcgtga gcggtcgacg agccggtggc cgaaggcgtc
      421 gacgatggac tgaccgaggc gctggccgac ggggctggcc cggacgtcgt cggcccggac
      481 gtcgtcggcc cggacgtcgt ccccggcccg agcggagccg gagtcgcgat cgtgggctgc
      541 gacgtcacga ccgtgcgcac gccgatggct ccggccgcca ctgcgaccag cgcgacggcg
      601 gccaccgccg cctgccgggt gcggcgcacc cgtcggccct tgcgcaccac ggtctcgccc
      661 agggtcgggt ccgccgattc tgcgggagcg tgctccgcga gggattgccg aatgagatcg
      721 tccaggtcac gagtcacgat ttgctccttc caagcaggta cgcaggttgc cgagggcacg
      781 ggacagctgg ctcttcaccg tgccgaccga gatgccgagt tccttggcga tggtgttgag
      841 gtcgagatcc tcgtagtagc gcagtacgac gatcgcccgt tgccgccggc cgagcctgga
      901 caggcccctc cagatcaggt cgcgctcgac cgcgtccgac gtcggtgggg ccgaggtctc
      961 aggcaccgcc gccaccgcga cctcgcggcg tccccacgat cgccacagcg acacatgggt
     1021 acgcgtcatg gtggttcgcg cgtaaccgcc gaggtggtcc cggtcgctca accggtccca
     1081 gaccagatac agcttcacca gggtggcctg cagcagatcg tcggcgtccg ggccgttgcc
     1141 ggtgagggca ggggcgtagc gacgcagggc gacctgctcg ccggccacaa agcgcgcaaa
     1201 tcccctgtcc tggcccagca cctgctgggt cggtgttatt gccatcgcca tgcctcacac
     1261 acgttcggcc gcgcccgaaa ggttgcaacc accctgccac gatctctgcc gagcaggcgc
     1321 gccccggtgt gacagcagtg acccgggccc cggtgtcgtg tcgagggcga cccactgcac
     1381 cctgccgacc agcagcggtt cttcgacact atggtgtcgc ccatgacaga acttcccgcc
     1441 gctggcgtcc gctccgaggt cggccggctg cgcacggtga tgctgcaccg tcccggcaat
     1501 gagcttcgcc ggctcacccc ccgcaacaac gatgcgctgc tgttcgacgg cctgccgtgg
     1561 gtggatcgtg cccaagcgga gcatgacgcc ttcgcggacg tgctgcgctc ccgcggcgtc
     1621 gaggtgctct acctcagcga actgctgatc gccacgctcg ccgaccccga cgccaggcag
     1681 gtcgcgatcg cgcacacgat gaccgacctg aggctcggtg acacgctgcg gcgctatctg
     1741 agcgacctgc tgggcgatca gggtccggac gaactggccg agattctgat ggccggcctg
     1801 cgcaacgacg aggtggcgag cgacaccggc agcctggtgg cggcgttgca cgcacccgac
     1861 gactttctga tcgacccgtt gcccaacctg ctgttcaccc gggattcgtc ggtgtgggtg
     1921 ggtgaccggg cgaccgtgac cgcgctggcg atgccggccc ggcggcgcga gacccagttg
     1981 accgaactga tctaccagtt ccatccacgc ttcgccgcga ccgagcgcat ccacggctgg
     2041 gacaaggaac tactcgaggg cggcgacgtg ctcgcgctgg ccgagggcgt gctggcggtc
     2101 ggggtcgggg aacgcacccg tccggccggc gccgagcggt tggcccgtac cgcgttcgac
     2161 gaagggctgg cgcacacggt gctggccgtg ccgatcgacc aggaccgcgc caccatgcac
     2221 ctggacacca tctgcaccat ggtcgacgcc gacgcggtgg tgatgtatcc caacgtcgcg
     2281 caccggctca ccgccgttac catcaagccc gacgaggacg gcgacctggt gatcgaacca
     2341 ccggagccgt tcgtcgtcgc ggccgcgcgc gccatgggca tcgatcagtt gcggctgatc
     2401 gacaccgggc tcgacccggt caccgccgag cgcgagcaat gggacgacgg caataacacc
     2461 ctggccatcg agccgcgggt ggcgatcgcc tatgagcgca acgtcgagac aaacgaccgg
     2521 ctcgccgccg ccggcatcga ggtgatcccg atcgccggct ccgagctggg gtccggacgg
     2581 ggcggtccgc gctgcatgtc gtgcccgatc agtcgcgacg cgctctgagc ctggatccac
     2641 cgatctgcgc ctactgcgcg gccccatccg ggcaccctgg ctgcgtcggc caggtctcac
     2701 cagacacccg gtctgccttc gcgcggaccg gcttgccagg ccacccgtct ggtgccgctc
     2761 gtgacgacgc ccatcggtgg ccaggctgag ccgccacctt ttggcggtta tcagggcagg
     2821 tggctgtcac ctggccggcc gccagcgtga gtcgccactt tctcgtggct acggataccc
     2881 cgccggggta tctattgcaa ccagaaagtg gcggctcgag gacggctgag gtgataaccg
     2941 ccgaaaagtg gcggttgagg ctcagacccg acgcagccag ccgtgggcgt ccggcacccg
     3001 cccgtactgg atgtcgagca ggtgcgcccg aatggccagg ctgagcgcgc cgggggtacc
     3061 gtcaccgacg gtgaaccgac gatcggtgcc gttgactccc ggggccgcga agcccaccac
     3121 cggcgtgacc acggcggccg tgccgcaggc gaacacctca ctgatcgctc cggaggtgat
     3181 gccgtcgaag acctcgtcga tgcccacctg ccgctgcacc ggacgcagcc cgtgctcggg
     3241 cgcgagggtc aggatcgagt cgcgggtgac gccgtccaga atcgtgccgg tcagcgcggg
     3301 ggtcaccaga tcgccctcgg ctgtgacgaa catgatgttc atcgttccgc actcctccac
     3361 ccaacggtgt tcggcgccgt ccagccagat cacctgctga cagccgtgtt cggcggcctc
     3421 gtcggccgcg gccaggctcg ctgcgtagtt gccgccgcat ttggccgctc cggttccgcc
     3481 ggccgccgcg cgggtgtaat tgggcgtggc ccagagggtc accgggtcgg ggtagtacgg
     3541 ccccaccggt gaggcgatca cgcagaacgt gtactcacgc g
//
LOCUS       DAOSUG010000322         3574 bp    DNA     linear   ENV 10-JUN-2024
DEFINITION  MAG TPA_asm: Micropruina sp. isolate AMR_MDS_2195
            NODE_10714_length_3574_cov_5.000568, whole genome shotgun sequence.
ACCESSION   DAOSUG010000322 DAOSUG010000000
VERSION     DAOSUG010000322.1
DBLINK      BioProject: PRJNA925477
            BioSample: SAMN32864262
            Sequence Read Archive: SRR4236673
KEYWORDS    WGS; ENV; Metagenome Assembled Genome; MAG; Third Party Data; TPA;
            TPA:assembly.
SOURCE      Micropruina sp. (wastewater metagenome)
  ORGANISM  Micropruina sp.
            Bacteria; Bacillati; Actinomycetota; Actinomycetes;
            Propionibacteriales; Nocardioidaceae; Micropruina.
REFERENCE   1  (bases 1 to 3574)
  AUTHORS   Abdulkadir,N., Saraiva,J.P., Zhang,J., Stolte,S., Gillor,O.,
            Harms,H. and Rocha,U.
  TITLE     Genome-centric analyses of 165 metagenomes show that mobile genetic
            elements are crucial for the transmission of antimicrobial
            resistance genes to pathogens in activated sludge and wastewater
  JOURNAL   Microbiol Spectr 12 (3), e0291823 (2024)
   PUBMED   38289113
REFERENCE   2  (bases 1 to 3574)
  AUTHORS   Abdulkadir,N.
  TITLE     Direct Submission
  JOURNAL   Submitted (24-MAR-2023) Environmental Microbiology, Helmholtz
            Center for Environmental Research -UFZ, Permoserstr 15, Leipzig
            04318, Germany
COMMENT     The annotation was added by the NCBI Prokaryotic Genome Annotation
            Pipeline (PGAP). Information about PGAP can be found here:
            https://www.ncbi.nlm.nih.gov/genome/annotation_prok/
            
            ##Genome-Assembly-Data-START##
            Assembly Method        :: SPAdes v. 3.11.1
            Genome Representation  :: Full
            Expected Final Version :: Yes
            Genome Coverage        :: Not Applicable
            Sequencing Technology  :: Illumina MiSeq
            ##Genome-Assembly-Data-END##
            
            ##Genome-Annotation-Data-START##
            Annotation Provider               :: NCBI
            Annotation Date                   :: 05/04/2023 04:57:10
            Annotation Pipeline               :: NCBI Prokaryotic Genome
                                                 Annotation Pipeline (PGAP)
            Annotation Method                 :: Best-placed reference protein
                                                 set; GeneMarkS-2+
            Annotation Software revision      :: 6.5
            Features Annotated                :: Gene; CDS; rRNA; tRNA; ncRNA
            Genes (total)                     :: 2,776
            CDSs (total)                      :: 2,750
            Genes (coding)                    :: 2,714
            CDSs (with protein)               :: 2,714
            Genes (RNA)                       :: 26
            tRNAs                             :: 24
            ncRNAs                            :: 2
            Pseudo Genes (total)              :: 36
            CDSs (without protein)            :: 36
            Pseudo Genes (ambiguous residues) :: 0 of 36
            Pseudo Genes (frameshifted)       :: 2 of 36
            Pseudo Genes (incomplete)         :: 32 of 36
            Pseudo Genes (internal stop)      :: 2 of 36
            ##Genome-Annotation-Data-END##
FEATURES             Location/Qualifiers
     source          1..3574
                     /organism="Micropruina sp."
                     /mol_type="genomic DNA"
                     /submitter_seqid="NODE_10714_length_3574_cov_5.000568"
                     /isolate="AMR_MDS_2195"
                     /isolation_source="Activated sludge of municipal
                     wastewater treatment plants"
                     /db_xref="taxon:2737536"
                     /environmental_sample
                     /geo_loc_name="Canada"
                     /lat_lon="49.25 N 123.1 W"
                     /collection_date="2015-06-16"
                     /metagenome_source="wastewater metagenome"
                     /note="metagenomic"
     gene            382..771
                     /locus_tag="PLF56_10310"
     CDS             382..771
                     /locus_tag="PLF56_10310"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_004810036.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_component: GO:0016020 - membrane [Evidence IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="DUF3817 domain-containing protein"
                     /protein_id="HOQ53993.1"
                     /translation="MITAAEVPAVRAALLRYRIMAWVVGVLLVVLVCVGVPLKYLGHN
                     DAVVTWTGVPHGWLYMILLITAYDLGRRVNWPWLRLILIALAGTVPFLSFVAERYATK
                     DVHRRLAEVTSDGYPQPSDAELERPAG"
     gene            complement(1044..1745)
                     /locus_tag="PLF56_10315"
     CDS             complement(1044..1745)
                     /locus_tag="PLF56_10315"
                     /inference="COORDINATES: protein motif:HMM:NF014193.2"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="SURF1 family protein"
                     /protein_id="HOQ53994.1"
                     /translation="MSLRFRQALIVALGIVAAAVMTWLGFWQLQVFTDQGNASVQARA
                     EQPAVPLIDQVGADGTVGDIYGKPVIANGHYLAGRQQRVLDEQGVVRVLSAFQLADGR
                     VLPVVRGVIAQGVDAPPAPAGHLTQTGLFLASEAGADHALARGYDLGSVRLAQLAQLW
                     PEPLIPGFITLDSAEAARQGLQPAAVNLPTGEGSWRNSGYALQWWVFALFALGMTIKI
                     AQGMGKRERTRQSSQ"
     gene            complement(1747..2706)
                     /locus_tag="PLF56_10320"
     CDS             complement(1747..2706)
                     /locus_tag="PLF56_10320"
                     /EC_number="6.3.2.4"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_011758114.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_function: GO:0005524 - ATP binding [Evidence IEA];
                     GO_function: GO:0008716 - D-alanine-D-alanine ligase
                     activity [Evidence IEA];
                     GO_function: GO:0046872 - metal ion binding [Evidence
                     IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="D-alanine--D-alanine ligase"
                     /protein_id="HOQ53995.1"
                     /translation="MSSDIGSIVVLAGGLSHERDVSLRSGRRVVQALRDAGQVVIEAD
                     VTGGLADLLADTDDPVVFPVLHGGDGEDGSLRQVLDLFAVPYVGSTGPASQRAFDKSI
                     CTPLLDVRAPAQVALPNSVFRELGARALVQAVAERLGLPVMVKPSRSGSALGCTRVDR
                     VEALPGALMAAYAYGEMAVIEQFIEGVEVAVTVIDTGEGPRALPAVEIRPRSGVYDYT
                     ARYTAGETRFLCPAEISDADARACADLALSVHGKLGLRDLSRTDIIIGAQGPTFIETN
                     VSPGMTETSLVPLSVEAAGLNFGELCVALVRQAHRRGASAHGR"
     gene            complement(2717..>3574)
                     /locus_tag="PLF56_10325"
     CDS             complement(2717..>3574)
                     /locus_tag="PLF56_10325"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_015583556.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_function: GO:0030170 - pyridoxal phosphate binding
                     [Evidence IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="PLP-dependent aminotransferase family protein"
                     /protein_id="HOQ53996.1"
                     /translation="IVQVAMDEFGIVPQALREAVAACRAAGKRVKFLYTIPNFQNPTG
                     VTQTLPRRRELIALAHELDLLIVEDNPYGLLTIEGEPLPALRSMDDERVIYLGSFSKT
                     FAPGFRIGWALAPHAVREKLTLAQESATLAPPVFSQYAIATYLHEFDWRAQIVAYRDM
                     YRGRRDAMLRGLVDNMPAGCTWTRPTGGFFVWLTMPDGLDSQAMLPRAVSARVAYVPG
                     TAFYADGLGSRNLRLSYCFPSEERILEGTRRLGEVLHDELDLLHTFGIGSAEHLYRDI
                     TAGPAPDIS"
BASE COUNT          596 a         1189 c         1218 g          571 t
ORIGIN      
        1 tggcgatcaa ccgagctagg aaagagccga ccaacgatcc gttggtgagc gggtcgaaaa
       61 ccaggagcgc tcaggtcgcg agccggtcga ggccaggttt ggacgcgctc gcacaggttg
      121 gacgagcccg ggcttggacg cgctcgtaca gtttgttcga ggccaggttt ggacgcgctc
      181 gcacaggttg gacgagccca ggtttggaca cactcgcaca ggtgttgggc gaggcgaggt
      241 atgtgatcga gagccgcgac acccaccaag attaccgata aatagtcttg acgctttatc
      301 gatatttctg gtcaggctga gcgtcctcgc ctccctggga atggcgaatc acggcctgcg
      361 tggaccggta gcctcgatcg ggtgatcacc gctgccgaag tgcccgccgt ccgcgccgcc
      421 ctgttgcgct atcgaatcat ggcctgggtg gtcggggtac tgctggtcgt gctggtctgc
      481 gtcggcgtgc cgctgaagta cctgggtcac aacgacgccg tggtgacgtg gacgggtgtg
      541 ccgcacggtt ggctgtacat gattctgctg atcaccgcct acgacctggg ccgtcgggtg
      601 aactggccct ggctacgcct gatactgatc gcactggcag gaaccgtccc gttcctttcg
      661 tttgtcgccg agcgctacgc gaccaaagac gtgcatcgca ggctggctga ggtgaccagt
      721 gatggctacc cgcagccgag cgacgccgaa ctcgagcggc ccgcgggctg atcgccccat
      781 catgggctga gcccgcatcc ggtcggcgtc agtgagcggg caggcgcagc cagtacgtcc
      841 ggacgggcgt gtaccaggct gtcgtcggtg gatcagcgac ggacgcactg cgcaggtgcg
      901 cgatccgctg gtcgagctcg cagcgagccc ctgttgccag gcgatctcga catcgccgct
      961 cgatggcggg atacctcgcc gatttcgtgt tgcaacccga aagcggacgg ccttcccgcc
     1021 accgcacagc gatctcgaca tcgctactgg cttgattgac gggttcgctc gcgcttgccc
     1081 atgccttggg cgatcttgat cgtcatgccg agtgcgaaca aggcgaacac ccaccattgc
     1141 agggcatacc cactgttgcg ccacgagccc tcaccggtgg ggaggttcac ggcggccggt
     1201 tgaaggccct gccgtgccgc ctccgccgag tcgagcgtga tgaatcccgg gatcaagggt
     1261 tcgggccaca gctgcgcgag ctgcgccaga cgcaccgagc ccaggtcata cccccttgcc
     1321 agggcatggt ccgcgccggc ctcggaggcc aggaacagtc cggtctgggt gaggtgcccg
     1381 gccggtgcgg gcggagcgtc cacgccttgt gcaatgacgc cgcggaccac cggcagcacc
     1441 cgtccgtcgg ccaactggaa ggccgagagc acgcgtacca cgccctgctc gtcgagtacc
     1501 cgctgctgcc gaccggccag gtagtgaccg ttcgcgatca ccggcttgcc gtagatgtcg
     1561 ccgaccgtgc cgtcggcgcc cacctggtcg atcagcggta cggcgggttg ctcggcccgc
     1621 gcctgaaccg aggcgttgcc ctggtcggtg aacacctgca gctgccagaa ccccagccac
     1681 gtcatcaccg cggcggcgac gatgcccagc gcgacgatca gtgcctgtcg aaaacgaagt
     1741 gacatctcag cgtccgtgcg ccgacgcacc ccgacgatgc gcctgacgca ccagggccac
     1801 gcacagctcg ccgaagttca gcccggccgc ctcgaccgac agcggtacga gagaggtttc
     1861 ggtcatgccg ggggagacgt tggtttcgat gaaggtgggc ccctgggcgc cgatgatgat
     1921 gtcggttcga gacaggtcgc gcagccccag cttcccgtgc acgctgagcg cgagatcggc
     1981 acaggctcgg gcgtcggcgt ccgagatctc ggcggggcag agaaagcgcg tctcgccggc
     2041 ggtgtagcgg gcggtgtagt cgtacacccc tgagcgtgga cggatctcga ccgcaggcaa
     2101 cgcccgcggc ccctcgccgg tgtcgatcac cgtgaccgcc acctcgacgc cctcgatgaa
     2161 ctgttcgatc acggccatct cgccgtaggc gtaggcagcc atgagcgcac cggggagcgc
     2221 ctcgacccga tcgaccctgg tgcacccgag cgccgatccg ctgcggctgg gcttgaccat
     2281 caccggtagt ccgagccgtt cggcgacggc ctgcaccaga gcccgtgcgc ccagctcgcg
     2341 aaacaccgag ttgggcaacg ccacctgagc gggcgcgcgc acgtccagca ggggggtgca
     2401 gatcgacttg tcgaaggctc gttgcgaggc gggtccggtg ctgcccacat aggggaccgc
     2461 gaacagatcc agtacctggc gcagcgaccc gtcttcgccg tcgccgccgt gcagcaccgg
     2521 aaacaccacc gggtcgtcgg tgtcggcgag caggtcggcg agtcccccag tgacgtcggc
     2581 ctcgatcacc acctgtcccg cgtcgcgcag ggcctggacc acccgccgtc cggagcgcaa
     2641 cgacacgtcg cgctcgtggg acaggccgcc ggccagaacg acgatgctgc cgatgtcgct
     2701 gctcatgggt gatccttcac gagatgtcgg gggccgggcc ggcagtgatg tcgcggtaca
     2761 ggtgctcagc cgagccgatg ccgaacgtgt gcagcaggtc gagttcgtcg tgcagcacct
     2821 cgcccaggcg ccgggtgcct tcgagtatgc gctcctcact gggaaagcag tacgacagcc
     2881 tcaggttgcg gctgcccaac ccgtcggcgt agaacgcggt acccggcacg tacgcgaccc
     2941 gggccgagac ggcacgcggc agcatcgcct gcgagtccag gccgtccggc atggtcagcc
     3001 agacgaagaa gccgccggtg ggacgggtcc acgtgcagcc ggccggcatg ttgtcgacca
     3061 gaccgcgcag catcgcgtcg cgtcgccccc ggtacatatc gcggtaggcg acgatctggg
     3121 cgcgccagtc gaactcgtgc aggtaggtgg cgatcgcgta ctggctgaac accgggggag
     3181 ccagggtcgc cgactcctgc gccagggtca gtttctcgcg gacggcgtgc ggcgccagtg
     3241 cccagccgat ccgaaacccg ggcgcgaagg tcttcgagaa cgaaccgaga tagatcaccc
     3301 gctcgtcgtc catcgagcgc agcgccggca gcggctcgcc ctcgatggtg agcagtccgt
     3361 acgggttgtc ctcgacgatc agcaggtcga gctcgtgtgc cagtgcaatc agttcgcggc
     3421 ggcgcggaag ggtctgggtc acgcccgtcg ggttttgaaa gttgggaatc gtgtacagga
     3481 acttgacccg cttgccagcg gcacgacacg ctgcgaccgc ctcacgcaag gcctgcggca
     3541 cgatgccgaa ctcgtccatc gccacctgga cgat
//
LOCUS       DAOSUG010000323         3561 bp    DNA     linear   ENV 10-JUN-2024
DEFINITION  MAG TPA_asm: Micropruina sp. isolate AMR_MDS_2195
            NODE_10752_length_3561_cov_4.260696, whole genome shotgun sequence.
ACCESSION   DAOSUG010000323 DAOSUG010000000
VERSION     DAOSUG010000323.1
DBLINK      BioProject: PRJNA925477
            BioSample: SAMN32864262
            Sequence Read Archive: SRR4236673
KEYWORDS    WGS; ENV; Metagenome Assembled Genome; MAG; Third Party Data; TPA;
            TPA:assembly.
SOURCE      Micropruina sp. (wastewater metagenome)
  ORGANISM  Micropruina sp.
            Bacteria; Bacillati; Actinomycetota; Actinomycetes;
            Propionibacteriales; Nocardioidaceae; Micropruina.
REFERENCE   1  (bases 1 to 3561)
  AUTHORS   Abdulkadir,N., Saraiva,J.P., Zhang,J., Stolte,S., Gillor,O.,
            Harms,H. and Rocha,U.
  TITLE     Genome-centric analyses of 165 metagenomes show that mobile genetic
            elements are crucial for the transmission of antimicrobial
            resistance genes to pathogens in activated sludge and wastewater
  JOURNAL   Microbiol Spectr 12 (3), e0291823 (2024)
   PUBMED   38289113
REFERENCE   2  (bases 1 to 3561)
  AUTHORS   Abdulkadir,N.
  TITLE     Direct Submission
  JOURNAL   Submitted (24-MAR-2023) Environmental Microbiology, Helmholtz
            Center for Environmental Research -UFZ, Permoserstr 15, Leipzig
            04318, Germany
COMMENT     The annotation was added by the NCBI Prokaryotic Genome Annotation
            Pipeline (PGAP). Information about PGAP can be found here:
            https://www.ncbi.nlm.nih.gov/genome/annotation_prok/
            
            ##Genome-Assembly-Data-START##
            Assembly Method        :: SPAdes v. 3.11.1
            Genome Representation  :: Full
            Expected Final Version :: Yes
            Genome Coverage        :: Not Applicable
            Sequencing Technology  :: Illumina MiSeq
            ##Genome-Assembly-Data-END##
            
            ##Genome-Annotation-Data-START##
            Annotation Provider               :: NCBI
            Annotation Date                   :: 05/04/2023 04:57:10
            Annotation Pipeline               :: NCBI Prokaryotic Genome
                                                 Annotation Pipeline (PGAP)
            Annotation Method                 :: Best-placed reference protein
                                                 set; GeneMarkS-2+
            Annotation Software revision      :: 6.5
            Features Annotated                :: Gene; CDS; rRNA; tRNA; ncRNA
            Genes (total)                     :: 2,776
            CDSs (total)                      :: 2,750
            Genes (coding)                    :: 2,714
            CDSs (with protein)               :: 2,714
            Genes (RNA)                       :: 26
            tRNAs                             :: 24
            ncRNAs                            :: 2
            Pseudo Genes (total)              :: 36
            CDSs (without protein)            :: 36
            Pseudo Genes (ambiguous residues) :: 0 of 36
            Pseudo Genes (frameshifted)       :: 2 of 36
            Pseudo Genes (incomplete)         :: 32 of 36
            Pseudo Genes (internal stop)      :: 2 of 36
            ##Genome-Annotation-Data-END##
FEATURES             Location/Qualifiers
     source          1..3561
                     /organism="Micropruina sp."
                     /mol_type="genomic DNA"
                     /submitter_seqid="NODE_10752_length_3561_cov_4.260696"
                     /isolate="AMR_MDS_2195"
                     /isolation_source="Activated sludge of municipal
                     wastewater treatment plants"
                     /db_xref="taxon:2737536"
                     /environmental_sample
                     /geo_loc_name="Canada"
                     /lat_lon="49.25 N 123.1 W"
                     /collection_date="2015-06-16"
                     /metagenome_source="wastewater metagenome"
                     /note="metagenomic"
     gene            100..381
                     /locus_tag="PLF56_10330"
     CDS             100..381
                     /locus_tag="PLF56_10330"
                     /inference="COORDINATES: protein motif:HMM:NF024242.2"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_function: GO:0003700 - DNA-binding transcription factor
                     activity [Evidence IEA];
                     GO_function: GO:0043565 - sequence-specific DNA binding
                     [Evidence IEA];
                     GO_process: GO:0006355 - regulation of DNA-templated
                     transcription [Evidence IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="helix-turn-helix transcriptional regulator"
                     /protein_id="HOQ53997.1"
                     /translation="MDRLRYDETSPALDELADLVGMSSHQFVRAMKRATGLAPLAWRQ
                     NARIVQARHLLREGTPIAETAHELGFFDQSHFHRVFRAHVAASPGIYRS"
     gene            461..1078
                     /locus_tag="PLF56_10335"
     CDS             461..1078
                     /locus_tag="PLF56_10335"
                     /inference="COORDINATES: protein motif:HMM:NF013933.2"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="LysE family translocator"
                     /protein_id="HOQ53998.1"
                     /translation="MDQFIAVAIAHFLALLIPGVDFFLIARTAMTSGWRSATGVCVGI
                     ATANALLITAAFSGISLISHPVLLDAIQLAGGGFLIFIGIAFLRATLQMDLNHDSDAE
                     NATWFRNLGLGLTSGLLNPKNALFYVSLAAAVSAASPTALVLYGVWMVSVLLIWDMFV
                     AVTLGSARALERMGRFLPWLTRLAGGFLVLFGAGMIVSLATQLTR"
     gene            complement(1459..>3561)
                     /locus_tag="PLF56_10340"
     CDS             complement(1459..>3561)
                     /locus_tag="PLF56_10340"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_002534834.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_function: GO:0005524 - ATP binding [Evidence IEA];
                     GO_function: GO:0016887 - ATP hydrolysis activity
                     [Evidence IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="ATP-dependent Clp protease ATP-binding subunit"
                     /protein_id="HOQ53999.1"
                     /translation="LNRVRNQVLQLLSGFQGKEAATSGAPETSQQQQGSSILDQFGRN
                     LTQAARENKLDPVIGREKEIERVMTVLSRRTKNNPVLIGEPGVGKTAVVEGLSQAIVR
                     GDVPETLRDKAIYTLDLGALVAGSRYRGDFEERLKKVLKEIKTRGDVVLFIDEIHTLV
                     GAGAAEGAIDAASILKPMLARGELQTIGATTLDEYRKYIEKDAALERRFQPIQVAEPS
                     IALTIDILRGLRDRYEAHHRITITDAALTASAQLADRYISDRFLPDKAIDLIDEAGAR
                     LRIRRMTAPPDLREFDERIAKVRLEKEAAIDAQDFERAARLRDDERKLLIARSEKEEA
                     WKVGDSDLPAEVGEEEIAEVLSSATGIPVFKLTEEESSRLLKMEEEIGRRYIGQHDAV
                     KALSRSIRRTRAGLKDPKRPSGSFIFAGPSGVGKTELTKALTEFLFGDEDALITLDMS
                     EYSEKHTASRMFGSPPGYVGYEEGGQLTEKVRRKPFSVVLFDEIEKAHPDIFNSLLQI
                     LDEGRLTDAQGRVVDFKNTVIVMTTNLGTRDISKSVNLGFSQSDDSVGSYEKMKAKVS
                     DELKQHFRPEFLNRVDEIVVFHQLSQGDIEHIVDLMVAEIENRLRDKDMGIELTHAAK
                     ALIAKRGFDPVLGARPLRRAIQRDIEDILAEKILFADIHPGEIVVVDVAPEGSELAFS
                     FNGVPKAEMPDVPAELTN"
BASE COUNT          569 a         1189 c         1114 g          689 t
ORIGIN      
        1 gggttcgcag ccctgttccg tctgctcgac ggagcctcgc caacgcatct tgtcaccacg
       61 gacgcaagcc caggactcct cgctcggttg cgtccggtta tggaccggct ccggtacgac
      121 gagacgagcc cggcgttgga cgagttggct gatctggttg ggatgtcgtc ccatcagttc
      181 gtgcgggcga tgaagcgcgc caccgggctc gcgccgttgg catggcggca gaacgcgcgc
      241 atcgtccagg cccggcacct gctgcgcgag ggcacaccga tcgccgagac cgcgcatgag
      301 ttggggttct tcgaccagag ccatttccac cgcgtcttcc gagcgcacgt cgctgcctca
      361 ccgggcatct accgcagttg actgcgcgaa acgtacaaga ccgccgcact gttcctcttc
      421 gatgcttgat gggtccgccc gtcacgacat ctggggagag atggatcagt tcatcgctgt
      481 cgctatcgcg cacttcctcg cgctgctgat ccccggcgtc gacttcttcc tcatcgcccg
      541 caccgccatg accagcgggt ggcgcagcgc caccggcgtg tgcgtgggca tcgcgacggc
      601 caacgccctc ctcatcacag ccgcattctc cggcatctcg ctgatctccc acccggtcct
      661 gctcgacgcg atccaactgg caggaggcgg gttcctcatc ttcatcggga tcgcgttcct
      721 ccgagccaca ctccagatgg acctgaacca cgattccgac gccgagaacg cgacctggtt
      781 tcgtaacctc gggctcggtc tcacctcagg gctgttgaat ccgaagaacg cgctgttcta
      841 cgtctctctg gccgccgccg tttctgcggc ttcgccgacg gcgctggtgc tgtacggggt
      901 ctggatggtc agcgtcctgc tgatctggga catgttcgtc gccgtcaccc ttggctccgc
      961 ccgagcgctg gagcgcatgg gacgtttcct gccctggctc acccgccttg caggcggctt
     1021 cctggtcctg ttcggagccg gcatgatcgt cagcctcgcc acacagctca cgcgatagac
     1081 cgacaaccaa ggcgccaggg caggttggct cgctgggtgt agcgggcgaa ggcggtagcg
     1141 acctcgctgg ttgcttgatg gactgcttcg tcgtcgcgta gcggtccgcc atgaaggatg
     1201 aactcagccc ggacccgacg atgggccgcc gcaaaactgc accagatgtg agaggtcgcg
     1261 tgcctcagcg gcgcgacagg ctgacgaatg tggtgcagtt tcgcacatca gcccgcattg
     1321 gacgcactca gccgaagccg cagcgggtgc gagcgagacc caatccgtga ttgagcggga
     1381 ccgtcccatg cggaaccggt cagcacgcag cacacccccc gcaacgctcg ttgcgggggg
     1441 tgtgctgcgg gtatcgactc agttggtgag ttcggccggc acgtcgggca tttcggcctt
     1501 cggcactccg ttgaagctga acgccaactc cgagccctcg ggcgccacat cgaccacgac
     1561 gatctcgccg gggtgaatgt cggcgaagag gatcttctcg gcgaggatgt cttcgatgtc
     1621 gcgctggatc gcccggcgca acggccgcgc ccccagcacc ggatcgaagc cacgcttggc
     1681 gatcagcgcc ttggcggcgt gggtcagctc gatgcccatg tccttgtcgc gcagccggtt
     1741 ctcgatctcg gcgaccatca ggtcgacgat gtgctcgata tcgccctgcg acaactggtg
     1801 gaaaacgacg atctcgtcca cgcggttgag aaactcgggg cggaagtgct gcttgagctc
     1861 gtccgacacc ttcgccttca tcttctcgta gctgccgaca ctgtcatcgg actggctgaa
     1921 ccccaggttg accgacttcg aaatgtcgcg ggtacccagg ttcgtggtca tcacgatcac
     1981 ggtgttcttg aagtcgacca cacgaccctg agcatcggtc agccgaccct cgtccagaat
     2041 ctgcagcagc gagttgaaga tatcggggtg agccttctcg atctcgtcga aaagcaccac
     2101 gctgaacggc ttgcgacgca ccttctcggt gagctggccg ccctcttcgt accccacata
     2161 tccgggcggt gaaccgaaca tccgcgacgc ggtgtgcttc tcggagtatt cgctcatgtc
     2221 gagagtgatc aaggcgtcct cgtcaccgaa cagaaactcg gtcagtgcct tggtcagctc
     2281 ggttttaccg acaccggagg gcccggcgaa gatgaacgag cccgacggac gcttggggtc
     2341 tttcagcccc gcgcgggtac gccggatcga gcgtgaaagc gccttgaccg cgtcatgctg
     2401 gccgatgtag cgcctaccga tctcttcttc catcttgagc agtcgggacg actcttcttc
     2461 ggtgagcttg aacaccggaa tgccggtggc cgagctgagc acctcggcga tctcttcttc
     2521 gccgacctcg gccggcagat cggaatcgcc gaccttccag gcctcttcct tctcggagcg
     2581 tgcgatcagc agcttgcgct cgtcatcgcg caggcgagcg gcccgttcga agtcctgcgc
     2641 gtcgatcgcg gcctctttct cgagccgcac cttcgcgatg cgctcgtcga actcgcgcag
     2701 gtcgggcggt gcggtcatgc gccggatgcg caaccgcgcg ccggcctcgt cgatcaggtc
     2761 gatggccttg tcgggcagga accgatccga gatgtaccgg tcggccaact gcgccgaagc
     2821 ggtcaacgcc gcgtccgtga tcgtgatgcg gtggtgtgcc tcgtagcggt cgcgcagccc
     2881 gcgcagaatg tcgatggtca gcgcgatgga cggctcggcg acctggatcg gctggaagcg
     2941 gcgctcgagc gccgcgtcct tctcgatgta cttgcggtac tcatccagcg tggtcgcgcc
     3001 gatcgtctgc agttcgccgc gggccagcat cggcttgaga atggacgcgg cgtctatggc
     3061 accctcggcg gcacccgcac ccaccagggt gtggatctca tcgatgaaca gcaccacgtc
     3121 accgcgggtc ttgatctctt tgagaacctt cttcaagcgc tcttcgaagt cgccgcggta
     3181 gcgcgagccg gccaccaacg cacccaggtc aagggtgtag atggccttgt cacgcagggt
     3241 ctcgggcacg tcaccgcgaa caatcgcctg cgacagcccc tcgaccacgg cggtcttgcc
     3301 cacaccgggc tcgccgatca gcaccgggtt gttcttggtg cgccgcgaca gcacggtcat
     3361 cacccgctcg atctctttct cgcggccgat caccgggtcg agcttgttct cgcgggcggc
     3421 ctgggtgagg ttgcgtccga actggtcgag aatcgacgaa ccctgctgct gctggctggt
     3481 ctcgggcgcg ccggacgtgg ccgcctcttt gccctggaag ccgctgagca actgcagcac
     3541 ctggttgcgc acccggttga g
//
LOCUS       DAOSUG010000324         3558 bp    DNA     linear   ENV 10-JUN-2024
DEFINITION  MAG TPA_asm: Micropruina sp. isolate AMR_MDS_2195
            NODE_10762_length_3558_cov_3.983157, whole genome shotgun sequence.
ACCESSION   DAOSUG010000324 DAOSUG010000000
VERSION     DAOSUG010000324.1
DBLINK      BioProject: PRJNA925477
            BioSample: SAMN32864262
            Sequence Read Archive: SRR4236673
KEYWORDS    WGS; ENV; Metagenome Assembled Genome; MAG; Third Party Data; TPA;
            TPA:assembly.
SOURCE      Micropruina sp. (wastewater metagenome)
  ORGANISM  Micropruina sp.
            Bacteria; Bacillati; Actinomycetota; Actinomycetes;
            Propionibacteriales; Nocardioidaceae; Micropruina.
REFERENCE   1  (bases 1 to 3558)
  AUTHORS   Abdulkadir,N., Saraiva,J.P., Zhang,J., Stolte,S., Gillor,O.,
            Harms,H. and Rocha,U.
  TITLE     Genome-centric analyses of 165 metagenomes show that mobile genetic
            elements are crucial for the transmission of antimicrobial
            resistance genes to pathogens in activated sludge and wastewater
  JOURNAL   Microbiol Spectr 12 (3), e0291823 (2024)
   PUBMED   38289113
REFERENCE   2  (bases 1 to 3558)
  AUTHORS   Abdulkadir,N.
  TITLE     Direct Submission
  JOURNAL   Submitted (24-MAR-2023) Environmental Microbiology, Helmholtz
            Center for Environmental Research -UFZ, Permoserstr 15, Leipzig
            04318, Germany
COMMENT     The annotation was added by the NCBI Prokaryotic Genome Annotation
            Pipeline (PGAP). Information about PGAP can be found here:
            https://www.ncbi.nlm.nih.gov/genome/annotation_prok/
            
            ##Genome-Assembly-Data-START##
            Assembly Method        :: SPAdes v. 3.11.1
            Genome Representation  :: Full
            Expected Final Version :: Yes
            Genome Coverage        :: Not Applicable
            Sequencing Technology  :: Illumina MiSeq
            ##Genome-Assembly-Data-END##
            
            ##Genome-Annotation-Data-START##
            Annotation Provider               :: NCBI
            Annotation Date                   :: 05/04/2023 04:57:10
            Annotation Pipeline               :: NCBI Prokaryotic Genome
                                                 Annotation Pipeline (PGAP)
            Annotation Method                 :: Best-placed reference protein
                                                 set; GeneMarkS-2+
            Annotation Software revision      :: 6.5
            Features Annotated                :: Gene; CDS; rRNA; tRNA; ncRNA
            Genes (total)                     :: 2,776
            CDSs (total)                      :: 2,750
            Genes (coding)                    :: 2,714
            CDSs (with protein)               :: 2,714
            Genes (RNA)                       :: 26
            tRNAs                             :: 24
            ncRNAs                            :: 2
            Pseudo Genes (total)              :: 36
            CDSs (without protein)            :: 36
            Pseudo Genes (ambiguous residues) :: 0 of 36
            Pseudo Genes (frameshifted)       :: 2 of 36
            Pseudo Genes (incomplete)         :: 32 of 36
            Pseudo Genes (internal stop)      :: 2 of 36
            ##Genome-Annotation-Data-END##
FEATURES             Location/Qualifiers
     source          1..3558
                     /organism="Micropruina sp."
                     /mol_type="genomic DNA"
                     /submitter_seqid="NODE_10762_length_3558_cov_3.983157"
                     /isolate="AMR_MDS_2195"
                     /isolation_source="Activated sludge of municipal
                     wastewater treatment plants"
                     /db_xref="taxon:2737536"
                     /environmental_sample
                     /geo_loc_name="Canada"
                     /lat_lon="49.25 N 123.1 W"
                     /collection_date="2015-06-16"
                     /metagenome_source="wastewater metagenome"
                     /note="metagenomic"
     gene            complement(<1..203)
                     /locus_tag="PLF56_10345"
     CDS             complement(<1..203)
                     /locus_tag="PLF56_10345"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_002518335.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_function: GO:0005524 - ATP binding [Evidence IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="ATP-binding cassette domain-containing protein"
                     /protein_id="HOQ54000.1"
                     /translation="MSLRFRATLAQRGFDVDLVVGARERVAVLGHNGSGKSTLLSILA
                     GTLRPDSGRATLDDRVLFDLDGRR"
     gene            complement(200..991)
                     /locus_tag="PLF56_10350"
     CDS             complement(200..991)
                     /locus_tag="PLF56_10350"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_013863309.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_component: GO:0043190 - ATP-binding cassette (ABC)
                     transporter complex [Evidence IEA];
                     GO_function: GO:0042626 - ATPase-coupled transmembrane
                     transporter activity [Evidence IEA];
                     GO_process: GO:0006810 - transport [Evidence IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="ABC transporter permease"
                     /protein_id="HOQ54001.1"
                     /translation="MARAYSGLPRWVYVPAGVGALFIVLPLVAMVTRLNWSRFGELIT
                     SESSVAALTLSLQTSAATMVICLILGVPMAMVLAREQFAGQRLVRSLVLLPLVLPPVV
                     GGIALLFTFGRRGMLAPVLNVFGISVAFSTAAVVLAQTFVALPFLVVSLEGTLRSVGQ
                     RYELVAATLGAKPSTVLRKVTLPLVVPGLASAAVLAFARALGEFGATITFAGSLQGVT
                     RTLPLEIYLQSVSDQDAAIALSLVLVVIAVVITVLTAKLSDRALI"
     gene            complement(995..1816)
                     /gene="modA"
                     /locus_tag="PLF56_10355"
     CDS             complement(995..1816)
                     /gene="modA"
                     /locus_tag="PLF56_10355"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_013863310.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_component: GO:0009897 - external side of plasma
                     membrane [Evidence IEA];
                     GO_component: GO:0030288 - outer membrane-bounded
                     periplasmic space [Evidence IEA];
                     GO_component: GO:0055052 - ATP-binding cassette (ABC)
                     transporter complex, substrate-binding subunit-containing
                     [Evidence IEA];
                     GO_function: GO:0015412 - ABC-type molybdate transporter
                     activity [Evidence IEA];
                     GO_function: GO:0030973 - molybdate ion binding [Evidence
                     IEA];
                     GO_process: GO:0015689 - molybdate ion transport [Evidence
                     IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="molybdate ABC transporter substrate-binding
                     protein"
                     /protein_id="HOQ54002.1"
                     /translation="MIPTTTPTKAKMALAVGAMALALAASACTAAPQASPTTTGGSAS
                     GTAKATLTVFAAASMQSTFTELQQRFQAAHPGTTITFNFAGSQALATQLVEGASADVF
                     VSANEAQMKVVTDAGLNAAAPTVFATNELTIAVPPDNPAGIRSLQDLTKKGLKLVVCA
                     PAVPCGAATQKLESATGLSFSPVSEEQAVTGVLAKVRSGEADAGLVYKTDVIAAGDAV
                     KGIDFPEASKAVNSNPIVLLKAAPNASAGQAWIDLVLSAEGQKVFTNAGFGPAKS"
     gene            complement(1828..2229)
                     /locus_tag="PLF56_10360"
     CDS             complement(1828..2229)
                     /locus_tag="PLF56_10360"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_008358984.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="TOBE domain-containing protein"
                     /protein_id="HOQ54003.1"
                     /translation="MTKLRIRDAAVFLGVSDDTVRRWIDAGLLSHEMDGSHRKVIDGR
                     DLATFAQSLANPMPDPSDVARSARNRFVGLVTKVVSDTVMSQVELQCGPFRVVSLMST
                     EAVRELGLEPGSVAVAVVKATTVIVETPHQN"
     gene            2512..3318
                     /locus_tag="PLF56_10365"
     CDS             2512..3318
                     /locus_tag="PLF56_10365"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_014847317.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="carbon-nitrogen hydrolase family protein"
                     /protein_id="HOQ54004.1"
                     /translation="MRVALAQFAATTDPAENLRQVQAAVERAAAADARLAVFPEAMIC
                     SFARPRIEAAEPLDGPWASAVRTAARQAGITVIVGMFTPGSSGKVRNTLLVTGPGIEA
                     HYDKLHLFDAYGYRESDQIEPGDELVLIEVDGIGIGLATCYDLRFPEQFKALAARGAD
                     IIVVPSSWAAGERKVDQWQVLVAARALDSTSIVVAVDQALPAAPHDHQDRKPTGVGHS
                     MVTGPLGELLLELGPEPELALVDLDVAAVASAREALPVLGNSRLTSPLTR"
     gene            3481..>3558
                     /gene="ffs"
                     /locus_tag="PLF56_10370"
     ncRNA           3481..>3558
                     /ncRNA_class="SRP_RNA"
                     /gene="ffs"
                     /locus_tag="PLF56_10370"
                     /product="signal recognition particle sRNA small type"
                     /inference="COORDINATES: nucleotide
                     motif:Rfam:14.4:RF00169"
                     /inference="COORDINATES: profile:INFERNAL:1.1.4"
                     /note="Derived by automated computational analysis using
                     gene prediction method: cmsearch."
                     /db_xref="RFAM:RF00169"
BASE COUNT          576 a         1232 c         1216 g          534 t
ORIGIN      
        1 cgcctgccgt ccaggtcgaa cagcacgcgg tcgtcgagag tggctctgcc cgagtccgga
       61 cgcagggtgc cggcgagaat gctcagcaag gtcgacttgc ccgacccgtt gtggccgagc
      121 accgccaccc gctcgcgcgc gccgaccacc aggtcgacat cgaagccgcg ctgggccagc
      181 gtggcccgga accgcaggct catatcagcg cccggtccga cagtttcgca gtgagcaccg
      241 tgatcaccac cgcgatcacc accagcacca gcgacaacgc gatcgcggcg tcctggtcgc
      301 tgaccgactg caggtagatc tccagcggca gggtgcgggt caccccctgc aggctgccgg
      361 cgaaggtgat ggtggcgccg aactcgccca gcgcacgggc gaaggcgagc acggccgccg
      421 aggccagccc cggcaccacc agcggcaggg tgaccttgcg caacacggtg gacggcttgg
      481 ctcccagcgt ggcggccacc agttcgtagc gttgccccac gctgcgcaga gttccctcga
      541 gcgaaaccac caggaacggc agcgcgacga aggtctgggc cagcaccacc gcggcggtgc
      601 tgaaggccac cgaaatgccg aacacgttca gcaccggcgc cagcatgccg cgccgtccga
      661 acgtgaacag cagggcgata ccgccgacca cgggcggcag caccagcggc agcagcacca
      721 gcgagcggac gaggcgctgg ccggcgaact gctcgcgggc cagcaccatc gccatcggca
      781 cccccaggat caggcagatc accatggtgg ccgccgaggt ctgcaggctc agcgtcaggg
      841 cggccaccga ggattcgctg gtgatcagtt cgccgaaccg gctccagttg agccgggtca
      901 ccatggcgac cagcggcagc acgatgaaca gcgcgccgac gccggccggc acgtacaccc
      961 agcgcggcag ccctgagtac gcccgtgcca tcagctagct ctttgcaggt ccgaatccgg
     1021 cgttcgtgaa gaccttctgc ccctcggcgc tgagcaccag atcgatccag gcctgcccgg
     1081 cactggcgtt cggtgcagcc ttgagcagca cgatcgggtt gctgttcacg gccttggacg
     1141 cctccggaaa gtcgatgccc ttcaccgcgt cgccggcggc gatcacgtcg gttttgtaga
     1201 ccaggcccgc gtccgcctcg ccggagcgca ccttggcgag taccccggtc accgcctgtt
     1261 cctcactgac cggactgaaa ctcaggccgg tggccgattc gagtttctgg gtggcggctc
     1321 cgcacggcac ggcaggtgcg cagaccacca gtttcaggcc cttcttggtc aggtcctgca
     1381 gcgagcggat gccggccggg ttgtcgggcg ggacggcgat cgtgagctcg ttggtggcga
     1441 agacggtcgg cgcggcagcg ttcaggccgg cgtcggtgac caccttcatc tgggcctcgt
     1501 tggccgacac gaacacgtcg gcggatgcac cctcgaccag ctgggtggcc agggcctgcg
     1561 agccggcgaa gttgaaggtg atggtggtgc ccgggtgggc ggcctggaag cgctgctgca
     1621 gttcggtgaa cgtggactgc atggacgccg ccgcgaacac cgtgagcgtt gccttggcgg
     1681 ttccagaggc gctgccaccc gtggtggtgg gggaggcctg cggcgcggcg gtgcaggcgg
     1741 acgcggccag ggccagggcc atcgcgccga cggcgagcgc cattttcgcc ttggtgggtg
     1801 tagtggtcgg gatcatggag agtcctctca gttctggtgg ggagtttcga cgatgacggt
     1861 ggtggccttg accactgcga ccgcgaccga gccgggttcc aggccaagtt cgcgcacggc
     1921 ctcggtgctc atcagcgaca ccacccggaa cgggccgcac tgcagctcga cctgcgacat
     1981 caccgtgtcc gagacgacct tggtgaccag gccgacgaac cggttacggg ccgagcgtgc
     2041 gacatcggac gggtcgggca tcggattggc cagcgactgc gcgaacgtag ccaggtctcg
     2101 accgtcgatc accttgcggt ggctgccgtc catctcatgg gacagcaggc cggcgtcgat
     2161 ccagcggcgc acggtgtcgt cgctcacgcc gaggaaaacc gccgcatcgc ggatccgcag
     2221 tttcgtcacg aacacatatt agggtccgcg gatgcgtctg tattgccggt ctatcgccgc
     2281 gagacgggag atcgatccca cgtgggaagc caatccggtc tgggtcgcgt cttcagaaaa
     2341 agccggtgcg tgcggcgacc cgaggccccg aggacgtagc tccgggtgca gccgtcgtgg
     2401 cgcacagcgc ctggtaatgg atcagctgca cgtgtgcggg atgacggtga ccgcacgtgg
     2461 tcggatcccg ccgacacgtc atggacgacc cgaccgtggc actctcactt catgcgcgtc
     2521 gctttggccc agttcgccgc caccaccgac cccgccgaga acctgcgcca ggtgcaggcc
     2581 gcagtggagc gcgccgcggc ggccgacgcc cgactggcgg tctttcccga ggcgatgatc
     2641 tgttcgttcg cccgtccacg catcgaagcc gccgagcccc tggacggtcc gtgggcctcg
     2701 gccgtgcgta ccgccgcacg gcaggccggc atcaccgtga tcgtcggaat gttcactccc
     2761 ggctcctccg gcaaggtgcg caacaccctg ttggtcaccg gtcccggcat cgaggcgcac
     2821 tacgacaagc tgcatctgtt cgatgcctac ggttaccgcg aatccgatca gatcgaaccg
     2881 ggggacgagc tcgtcctgat tgaggtggac ggcatcggga tcggcctggc cacctgttac
     2941 gacctgcgct tccccgagca gttcaaggcc ctggccgccc gcggagccga catcatcgtg
     3001 gtgccgtcgt cgtgggcggc gggggagcgc aaggtcgacc agtggcaggt gctggtcgcc
     3061 gcgcgcgccc ttgattcgac cagcatcgtg gtcgcggtcg atcaagcgct ccctgcggcg
     3121 ccgcacgatc accaggaccg caagcccacc ggcgtcgggc actcgatggt caccggccca
     3181 ttgggcgagc ttctgctcga actggggcca gaacccgaac tggccctcgt cgacctggac
     3241 gtcgccgcgg tcgcgtccgc gcgcgaagcc ctgccggtgc tcggcaactc gcggctcacc
     3301 agccccctga cgcggtagtc ctgaatcgtt gacgccgggc aaccggtcgc cgcggcacag
     3361 cgctgtcggc gcggcggtgc ccgtcggccc gaaagccggt agtcgccggg ccgctcgcaa
     3421 cgacgtgccg catcgggcaa ccgcgtcact ggtcaccctc cgacgtcccc tacactggca
     3481 acaaggtccc cgcgcggcgg tacctcgccc aactccccca gggtcgaaag acagcaaggg
     3541 taagtgagct cttccggg
//
LOCUS       DAOSUG010000325         3555 bp    DNA     linear   ENV 10-JUN-2024
DEFINITION  MAG TPA_asm: Micropruina sp. isolate AMR_MDS_2195
            NODE_10770_length_3555_cov_2.765429, whole genome shotgun sequence.
ACCESSION   DAOSUG010000325 DAOSUG010000000
VERSION     DAOSUG010000325.1
DBLINK      BioProject: PRJNA925477
            BioSample: SAMN32864262
            Sequence Read Archive: SRR4236673
KEYWORDS    WGS; ENV; Metagenome Assembled Genome; MAG; Third Party Data; TPA;
            TPA:assembly.
SOURCE      Micropruina sp. (wastewater metagenome)
  ORGANISM  Micropruina sp.
            Bacteria; Bacillati; Actinomycetota; Actinomycetes;
            Propionibacteriales; Nocardioidaceae; Micropruina.
REFERENCE   1  (bases 1 to 3555)
  AUTHORS   Abdulkadir,N., Saraiva,J.P., Zhang,J., Stolte,S., Gillor,O.,
            Harms,H. and Rocha,U.
  TITLE     Genome-centric analyses of 165 metagenomes show that mobile genetic
            elements are crucial for the transmission of antimicrobial
            resistance genes to pathogens in activated sludge and wastewater
  JOURNAL   Microbiol Spectr 12 (3), e0291823 (2024)
   PUBMED   38289113
REFERENCE   2  (bases 1 to 3555)
  AUTHORS   Abdulkadir,N.
  TITLE     Direct Submission
  JOURNAL   Submitted (24-MAR-2023) Environmental Microbiology, Helmholtz
            Center for Environmental Research -UFZ, Permoserstr 15, Leipzig
            04318, Germany
COMMENT     The annotation was added by the NCBI Prokaryotic Genome Annotation
            Pipeline (PGAP). Information about PGAP can be found here:
            https://www.ncbi.nlm.nih.gov/genome/annotation_prok/
            
            ##Genome-Assembly-Data-START##
            Assembly Method        :: SPAdes v. 3.11.1
            Genome Representation  :: Full
            Expected Final Version :: Yes
            Genome Coverage        :: Not Applicable
            Sequencing Technology  :: Illumina MiSeq
            ##Genome-Assembly-Data-END##
            
            ##Genome-Annotation-Data-START##
            Annotation Provider               :: NCBI
            Annotation Date                   :: 05/04/2023 04:57:10
            Annotation Pipeline               :: NCBI Prokaryotic Genome
                                                 Annotation Pipeline (PGAP)
            Annotation Method                 :: Best-placed reference protein
                                                 set; GeneMarkS-2+
            Annotation Software revision      :: 6.5
            Features Annotated                :: Gene; CDS; rRNA; tRNA; ncRNA
            Genes (total)                     :: 2,776
            CDSs (total)                      :: 2,750
            Genes (coding)                    :: 2,714
            CDSs (with protein)               :: 2,714
            Genes (RNA)                       :: 26
            tRNAs                             :: 24
            ncRNAs                            :: 2
            Pseudo Genes (total)              :: 36
            CDSs (without protein)            :: 36
            Pseudo Genes (ambiguous residues) :: 0 of 36
            Pseudo Genes (frameshifted)       :: 2 of 36
            Pseudo Genes (incomplete)         :: 32 of 36
            Pseudo Genes (internal stop)      :: 2 of 36
            ##Genome-Annotation-Data-END##
FEATURES             Location/Qualifiers
     source          1..3555
                     /organism="Micropruina sp."
                     /mol_type="genomic DNA"
                     /submitter_seqid="NODE_10770_length_3555_cov_2.765429"
                     /isolate="AMR_MDS_2195"
                     /isolation_source="Activated sludge of municipal
                     wastewater treatment plants"
                     /db_xref="taxon:2737536"
                     /environmental_sample
                     /geo_loc_name="Canada"
                     /lat_lon="49.25 N 123.1 W"
                     /collection_date="2015-06-16"
                     /metagenome_source="wastewater metagenome"
                     /note="metagenomic"
     gene            <1..514
                     /locus_tag="PLF56_10375"
     CDS             <1..514
                     /locus_tag="PLF56_10375"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_016669411.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=2
                     /transl_table=11
                     /product="hypothetical protein"
                     /protein_id="HOQ54005.1"
                     /translation="GDVPLDDVAQALQATITANGRTIEPGALGAAAVATGGYPFMIQL
                     VGYHTWRKASTNHLTTPAVEAGIQQARTRLGSLVHAAALRELSDVDRTFLLAMAQDQG
                     SSRTADIARRIGKDAQYTNVYRARLIAAGIITPAGYGKVQFAIPYLREYLVEHAAHQS
                     MASNDPPRLS"
     gene            complement(568..1995)
                     /locus_tag="PLF56_10380"
     CDS             complement(568..1995)
                     /locus_tag="PLF56_10380"
                     /inference="COORDINATES: protein motif:HMM:NF018613.2"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_function: GO:0016758 - hexosyltransferase activity
                     [Evidence IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="glycosyltransferase"
                     /protein_id="HOQ54006.1"
                     /translation="MGIRTLILTAGVGAGHNQAANAIQAALAELPEIDDVARIDTLDT
                     TPEAFNRLYDDAYFALVSDVPWLIGWGYDNQDAPFKLAPAVRWWDQLNTLSLVRAIRE
                     LDPDVVICTHFLPARLVALLLARGQLRASLTVATTDYDFQGLWLTTPFTHFFVAREET
                     RRFVMDLGVPGDRIAASGIPVRLGLGDPVDEAQVRQRFGLDPDLPVVLISAGAAGGSY
                     TVNTIQQALRVEHPFQAVVICGHNEELKREIDDLVAGRTDQFKVIGFTTEMADLLRIA
                     ALFVGKPGGLSSSECMAAGLPMVLVNPIPGQEVRNADYLVEEGAAVRCNYATTIGFKL
                     DALLADPERLRTMAANARRVGRADSARTVVNGAVQLLTPPLWVTREAQKSMQYVAEHG
                     VAAADLPDERALLTLTDPVTGGSRAVATRAQLRALGVSTWSHEVTLARETLRDPRWRQ
                     QNLDLAVATKWLLGDQESCTFGLSG"
     gene            complement(2075..2719)
                     /locus_tag="PLF56_10385"
     CDS             complement(2075..2719)
                     /locus_tag="PLF56_10385"
                     /inference="COORDINATES: protein motif:HMM:NF023144.2"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_function: GO:0046872 - metal ion binding [Evidence
                     IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="maleylpyruvate isomerase N-terminal
                     domain-containing protein"
                     /protein_id="HOQ54007.1"
                     /translation="MGRDPAVRRRDRRRSGGGRAGEAGASARGAGHAGVLSSVTRVLI
                     DELDARDDDQPAWSWFPSDQSVGFTRRMQVHEAVMHRIDAEVAAGLPVTPVDAEVAQA
                     GIVHAVDVMFAWWGTLPGFAFHPAEGVVALTLTDLGRTILLRPGRWRGVGQSGTSYDE
                     PGIVRVTELVPTAGWAGSADQVDRWLWGRGAEPESDGDEAALAQVREVVAKGIE"
     gene            complement(2602..2865)
                     /locus_tag="PLF56_10390"
     CDS             complement(2602..2865)
                     /locus_tag="PLF56_10390"
                     /inference="COORDINATES: protein motif:HMM:NF023144.2"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_function: GO:0046872 - metal ion binding [Evidence
                     IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="maleylpyruvate isomerase N-terminal
                     domain-containing protein"
                     /protein_id="HOQ54008.1"
                     /translation="MFDHTAVIATETQRFSVAIEGADPDAPVPTCPGWTVADLAWHLT
                     EVHAFWAEILRSGAVTDDDQEAVEQAKPERPREVPATLACCRP"
     gene            complement(2887..>3555)
                     /locus_tag="PLF56_10395"
     CDS             complement(2887..>3555)
                     /locus_tag="PLF56_10395"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_011755770.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="glycoside hydrolase family 15 protein"
                     /protein_id="HOQ54009.1"
                     /translation="LTALLKPPTIDWEIRSERRHFVHSKVMAWVAFDRMIRGVEKYGL
                     DGPVERWRALRQRARDEIDGRGVTNGHFTQYYGTDEVDASLLVLPAIGYCEPDDPRML
                     ATVERIERTLMVDGLLHRYRAETGVDGLAGTEHPFLACSFWLVEQYARTGRLDDAESL
                     LDRLCGVANDLGLLSEEYDVHDQRQAGNMPQAFSHLALVRAADALAAARALRDAGERG
                     RGTS"
BASE COUNT          566 a         1305 c         1168 g          516 t
ORIGIN      
        1 cggcgacgtc cccctcgacg acgtcgccca agccctccag gccaccatca ccgcaaacgg
       61 ccgaaccatc gaacccggcg ccctcggcgc cgccgcagtc gccaccggcg gatacccgtt
      121 catgatccaa ctcgtcggct accacacctg gcgcaaggcc agcacgaacc acctcaccac
      181 cccggcagtc gaagccggca tccagcaagc ccgcacccgg ctcggttcac tcgtccacgc
      241 cgccgccctg cgcgagctct ccgacgtcga ccggaccttc ctgctcgcca tggcccaaga
      301 ccagggcagc tcgcgcaccg ccgacatcgc acggcggatc ggcaaggacg cccagtacac
      361 caacgtctac cgggcacgac tcatcgccgc cggcataatc accccagccg gctacggcaa
      421 agtccagttc gcgatcccct acctacgcga gtacctcgtc gagcacgcag cccaccagtc
      481 catggccagc aacgacccgc cgcgcctctc ctgaaccgct acctcggggg gcaacccggc
      541 cacttgggta ggtagtcacc caggcagtca gcccgagagc ccgaaggtgc aggactcctg
      601 atcgcccagc agccacttcg tggcgacggc caggtcgagg ttctgctggc gccaacgggg
      661 gtcgcgcagt gtctcgcggg ccagggtcac ctcgtgcgac caggtgctga cgcccagcgc
      721 gcgcagttgt gcccgggtgg ccaccgcgcg tgagccgccg gtgaccggat cggtcagcgt
      781 gagcagggcg cgctcgtcgg gcaggtcggc tgcggccacc ccgtgctcgg ccacgtactg
      841 catggatttt tgcgcctcgc gggtgaccca cagcggcggg gtgagcagtt ggacggcccc
      901 gttcaccacc gtgcgcgcgg agtcggcgcg acccacccgt cgcgcgttgg cggccatcgt
      961 gcgcagccgc tcggggtcgg cgagcagcgc gtccagcttg aagccgatgg tggtggcgta
     1021 gttgcagcgc acggcggcgc cctcttcgac cagatagtcg gcgttgcgca cctcctggcc
     1081 gggaatcggg ttgaccagca ccatgggcaa cccggccgcc atgcactccg acgacgacaa
     1141 tccgcccggc ttgccgacga acagtgccgc gattcgcagc aggtcggcca tctcggtggt
     1201 gaaaccgatc accttgaact ggtcggtgcg gcccgcgacg agatcgtcga tctcgcgctt
     1261 gagttcttcg ttgtggccgc agatcaccac cgcctggaac gggtgttcga cgcgcagcgc
     1321 ctgctgaatg gtgttgacgg tgtagctgcc gccggcggcc ccggcagaga tcagcaccac
     1381 cggcaggtcg gggtcgagtc cgaagcgttg ccgcacctgc gcctcatcca ccggatcgcc
     1441 cagaccgagc cgcaccggaa tgcccgaggc ggcgatccgg tcgccgggta cgccgagatc
     1501 catcacgaac cggcgggtct cttcgcgcgc cacgaagaag tgggtgaacg gtgtggtcag
     1561 ccacaacccc tgaaagtcgt agtccgtggt ggccacggtc agggacgcgc gcaactggcc
     1621 acgggccagc aacaacgcca ccagccgggc cggcagaaag tgggtgcaga tcaccacgtc
     1681 ggggtcgagt tcacggatcg cgcgcacgag cgacagcgtg ttcagctggt cccaccagcg
     1741 caccgccggc gccagcttga agggggcgtc ctggttgtcg tagccccaac cgatcagcca
     1801 cggaacgtcc gacaccagag cgaagtaggc gtcgtcgtac agcctgttga acgcctcggg
     1861 ggtggtgtcg agggtgtcga tccgggcgac gtcgtcaatc tcgggcagtt ccgccagtgc
     1921 ggcctggatc gcgttggcgg cctggttgtg cccggcaccc acacctgcgg tcagaatcaa
     1981 ggtgcgaata cccattgcgc gagcctagtg gctggacttc gctgatcgag acgcgcgtcg
     2041 agaccattcg tgggccggca cgctcgacca acggctactc gattcccttg gccaccacct
     2101 cacgcacctg cgccagggcc gcctcgtcgc cgtccgactc gggttctgcg ccgcggcccc
     2161 acagccaccg gtccacctgg tcggcgctac cggcccaccc ggccgtcggc accaactcgg
     2221 tcacccggac gatgccgggc tcgtcgtagc tggtgcccga ctggccgacc ccgcgccagc
     2281 gtccgggacg cagcaggatc gtgcgcccca ggtcggtcag ggtgagggcc accaccccct
     2341 ctgcggggtg gaacgcgaag ccgggtagcg tgccccacca ggcgaacatc acatcgaccg
     2401 cgtgcacgat gcctgcctgc gccacctcgg cgtcgacggg ggtgaccggc aacccggccg
     2461 ccacttcggc gtcgatacgg tgcatcaccg cctcgtgcac ctgcatccgc cgggtgaagc
     2521 cgacgctctg gtcgctgggg aaccacgacc aggcgggctg gtcgtcgtcc cgcgcgtcca
     2581 gttcgtcgat cagcacccgg gtcacggacg acagcacgcc agcgtggccg gcacctcgcg
     2641 cggacgctcc ggcttcgcct gctcgaccgc ctcctgatcg tcgtcggtca cggcgccgga
     2701 ccgcaggatc tcggcccaga acgcgtgcac ctcggtcagg tgccaggcca ggtcggcgac
     2761 cgtccagccg gggcaggtgg gaaccggagc gtcgggatcg gcaccctcga tcgcgacgct
     2821 gaagcgctgg gtctcggtgg cgatgactgc tgtgtgatcg aacatgcggc cgacgctacg
     2881 ccgcaactag gacgttccgc gtcctcgttc gccggcatcc cgaagcgcac gagccgccgc
     2941 cagtgcgtcc gcggcccgca ccagcgccag gtgcgagaac gcctgcggca tgttgccggc
     3001 ctgccgctgg tcgtgcacgt cgtactcctc cgacagcagg cccaggtcgt tggccacgcc
     3061 acagagtcgg tcgagcagcg actcggcgtc gtcgaggcgt ccggtgcggg cgtactgctc
     3121 caccagccag aacgaacagg ccagaaacgg atgctcggtg cccgccaggc cgtccacccc
     3181 ggtttcggcc cgatagcgat gcagcagccc gtccaccatc agggtgcgtt cgatccgctc
     3241 cactgtggcc agcatgcggg ggtcgtcggg ttcgcagtag ccgatcgccg gcagcaccaa
     3301 cagcgaggcg tccacctcgt cggtgccgta gtactgggtg aagtggccgt tggtcacgcc
     3361 ccggccgtcg atctcgtcgc gggcccgttg ccgcagcgcg cgccagcgtt ccaccgggcc
     3421 gtccaggccg tacttctcga cgccccggat catccggtcg aaggccaccc aggccatcac
     3481 cttggagtgc acgaagtgcc gccgctcgga ccggatctcc cagtcgatgg tcggaggttt
     3541 gagcagggcc gtcag
//
LOCUS       DAOSUG010000326         3548 bp    DNA     linear   ENV 10-JUN-2024
DEFINITION  MAG TPA_asm: Micropruina sp. isolate AMR_MDS_2195
            NODE_10796_length_3548_cov_4.535643, whole genome shotgun sequence.
ACCESSION   DAOSUG010000326 DAOSUG010000000
VERSION     DAOSUG010000326.1
DBLINK      BioProject: PRJNA925477
            BioSample: SAMN32864262
            Sequence Read Archive: SRR4236673
KEYWORDS    WGS; ENV; Metagenome Assembled Genome; MAG; Third Party Data; TPA;
            TPA:assembly.
SOURCE      Micropruina sp. (wastewater metagenome)
  ORGANISM  Micropruina sp.
            Bacteria; Bacillati; Actinomycetota; Actinomycetes;
            Propionibacteriales; Nocardioidaceae; Micropruina.
REFERENCE   1  (bases 1 to 3548)
  AUTHORS   Abdulkadir,N., Saraiva,J.P., Zhang,J., Stolte,S., Gillor,O.,
            Harms,H. and Rocha,U.
  TITLE     Genome-centric analyses of 165 metagenomes show that mobile genetic
            elements are crucial for the transmission of antimicrobial
            resistance genes to pathogens in activated sludge and wastewater
  JOURNAL   Microbiol Spectr 12 (3), e0291823 (2024)
   PUBMED   38289113
REFERENCE   2  (bases 1 to 3548)
  AUTHORS   Abdulkadir,N.
  TITLE     Direct Submission
  JOURNAL   Submitted (24-MAR-2023) Environmental Microbiology, Helmholtz
            Center for Environmental Research -UFZ, Permoserstr 15, Leipzig
            04318, Germany
COMMENT     The annotation was added by the NCBI Prokaryotic Genome Annotation
            Pipeline (PGAP). Information about PGAP can be found here:
            https://www.ncbi.nlm.nih.gov/genome/annotation_prok/
            
            ##Genome-Assembly-Data-START##
            Assembly Method        :: SPAdes v. 3.11.1
            Genome Representation  :: Full
            Expected Final Version :: Yes
            Genome Coverage        :: Not Applicable
            Sequencing Technology  :: Illumina MiSeq
            ##Genome-Assembly-Data-END##
            
            ##Genome-Annotation-Data-START##
            Annotation Provider               :: NCBI
            Annotation Date                   :: 05/04/2023 04:57:10
            Annotation Pipeline               :: NCBI Prokaryotic Genome
                                                 Annotation Pipeline (PGAP)
            Annotation Method                 :: Best-placed reference protein
                                                 set; GeneMarkS-2+
            Annotation Software revision      :: 6.5
            Features Annotated                :: Gene; CDS; rRNA; tRNA; ncRNA
            Genes (total)                     :: 2,776
            CDSs (total)                      :: 2,750
            Genes (coding)                    :: 2,714
            CDSs (with protein)               :: 2,714
            Genes (RNA)                       :: 26
            tRNAs                             :: 24
            ncRNAs                            :: 2
            Pseudo Genes (total)              :: 36
            CDSs (without protein)            :: 36
            Pseudo Genes (ambiguous residues) :: 0 of 36
            Pseudo Genes (frameshifted)       :: 2 of 36
            Pseudo Genes (incomplete)         :: 32 of 36
            Pseudo Genes (internal stop)      :: 2 of 36
            ##Genome-Annotation-Data-END##
FEATURES             Location/Qualifiers
     source          1..3548
                     /organism="Micropruina sp."
                     /mol_type="genomic DNA"
                     /submitter_seqid="NODE_10796_length_3548_cov_4.535643"
                     /isolate="AMR_MDS_2195"
                     /isolation_source="Activated sludge of municipal
                     wastewater treatment plants"
                     /db_xref="taxon:2737536"
                     /environmental_sample
                     /geo_loc_name="Canada"
                     /lat_lon="49.25 N 123.1 W"
                     /collection_date="2015-06-16"
                     /metagenome_source="wastewater metagenome"
                     /note="metagenomic"
     gene            <1..2352
                     /locus_tag="PLF56_10400"
     CDS             <1..2352
                     /locus_tag="PLF56_10400"
                     /inference="COORDINATES: protein motif:HMM:NF024117.2"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_function: GO:0005524 - ATP binding [Evidence IEA];
                     GO_function: GO:0016787 - hydrolase activity [Evidence
                     IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="3'-5' exonuclease"
                     /protein_id="HOQ54010.1"
                     /translation="TFRGADIAAYTAAVDQSGARFSLSVNHRSDEALVHALGELFADL
                     QLGDGIEVHPVSAHRAGSGVRAATSSVHPVTLRAIDDEIEPWAAHERIRADLVAQVRA
                     VLDHQQLCDGDRWREVRPGDLAVLVRSNKGAARAAAALRAAGIPVALSGADSLYDSPA
                     CGDWLVLLQALATQRRHDVRHALISTFFGLSLSELAGADDAQLADWTATLRQWDRVLR
                     QSGVAALFASVCAETSFLERVLSRPDGERSMTDFRHLAEVLHEASGPLRPTAAWLVDW
                     LAAERGKWAENERTRRLETDAQAVQVKTVHGAKGLQYPIVLLPDLWALPGGADDGGRL
                     LYHDESGRAVVDLGGRDAAGRGRRLRRAEAEEAEESLRLLYVAFTRARSQVIAWWART
                     KKTPRSALQRVLYRPRTGRPSAPDAGYPLDRTPGTLSPAQLPWVRAASGVVVREVEAA
                     APAPPPLALHESPRLRALRFDRSIDADWRRTSYSGLTAGVHADLPLLADEPDDAESVV
                     APAGSSVPSPMRDLPGGTQFGSLVHQLFEHVDPGSDDTTLTARLRAAAAEWLPRFPLS
                     GVTSQALADALLPAFHTPLGPLADDRSLAQLPITDRLAELSFDLPMAPASGFTLGDVA
                     RLLREHLPTGDPLVGYPALLDAPALAGQPLKGFLTGSIDAVLRVGTPEHPRFVVVDYK
                     TNRLGGDDLTLGHYAHAPLVAAMCASHYPLQALLYCVALHRFLAARLVRYDPHRHLGG
                     VLYLFVRGMGGATAGPRTGVFAWQPPVTLIERLSRVLGGFSDD"
     gene            2345..>3548
                     /locus_tag="PLF56_10405"
     CDS             2345..>3548
                     /locus_tag="PLF56_10405"
                     /inference="COORDINATES: protein motif:HMM:NF024991.2"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="AAA family ATPase"
                     /protein_id="HOQ54011.1"
                     /translation="MTDPALSVATSGLTGEFHILGVLDWADLHPARQFTRLYHEPDER
                     VQLAAALTVRALRAGSVCLELDRVRTGVTGRDDELVDVPDELWPDPDDWLRALQASPL
                     VAQGPGGDDTLPLRLIDHRLYLTRYWQQEEQVRRSLQQRSSAHAGVAAPEQLAAWLDE
                     LFPGPNADPDQRQGVAQVTTSGVTVVAGGPGTGKTTTIARMIAVLLRQGVQRVALAAP
                     TGKAAARMDEALRAAAHDLPADVADRVAQVRASTLHRLLGAQPYRNPPFRHHRDNPLP
                     HDVVIVDELSMVSLTMMAQLTDALGVHTRLVLVGDPDQLSSVGAGAVLGDLTSAGWQG
                     TGVHNPVVRLRRNYRFAGVLGELAEAVRTGDAETVLRLVDGAEANLTLTGPDDPQHAL
                     RARVVHQGR"
BASE COUNT          463 a         1281 c         1275 g          529 t
ORIGIN      
        1 acctttcgcg gcgccgacat cgccgcctac accgccgcgg tggaccaatc cggcgctcgg
       61 ttctcgctca gcgtcaacca ccgcagcgac gaggccctgg tgcacgcgct gggcgagctg
      121 ttcgccgacc tgcaactggg cgacggcatc gaggtgcatc cggtcagtgc gcaccgcgcg
      181 ggctcggggg tgcgggccgc cacgtcgtcg gtacaccccg tcacgttgcg ggcgatcgac
      241 gacgagatcg aaccctgggc ggcccacgag cgcatccgcg ccgatctggt ggcgcaggtg
      301 cgcgccgttc tcgatcacca gcagctgtgc gacggcgatc gctggcgtga ggtgcgtccg
      361 ggcgacctgg ccgtgctcgt ccggtcgaac aaaggcgccg cccgggccgc cgcagccctg
      421 cgcgcggcag gcattccggt ggcgctcagc ggcgccgaca gcctctacga ctcgccggcc
      481 tgcggcgact ggctggtgct gctgcaggca ttggcgacgc agcgccgaca tgacgtgcgg
      541 cacgccttga tcagcacctt cttcggattg tcgctgagcg aactggccgg tgccgacgac
      601 gctcaactgg ccgactggac cgccaccctt cggcagtggg accgcgtgct gaggcagtcc
      661 ggggtggccg cgttgttcgc ctcggtctgc gccgagacgt cgtttctgga gcgggtactg
      721 tcccgccccg atggtgagcg ttccatgacc gacttccggc atctcgccga ggtgctgcac
      781 gaggcgtccg gtccgctgcg gcccaccgcg gcctggttgg tcgactggct ggccgccgaa
      841 cgtggcaagt gggccgagaa cgaacgcacc cggcggctcg agaccgacgc gcaggccgtc
      901 caggtcaaga ccgtgcacgg cgcgaagggg ttgcagtacc cgatcgtgct gctgcccgac
      961 ctgtgggcac tgcccggcgg cgccgacgac gggggccggc tgctgtacca cgacgaatcc
     1021 ggacgggcgg tggtcgatct gggcgggcgc gacgccgccg gacggggccg ccggctgcgt
     1081 cgagccgagg ccgaagaggc cgaagagtcg ttgcggctgc tctatgtggc gttcacccgg
     1141 gcacggtcac aggtgatcgc ctggtgggcg cgtaccaaga agacgccgcg ctcggcgctg
     1201 cagcgcgtgc tgtaccggcc acggacgggg cgcccgtccg cccccgatgc cggttatccg
     1261 ctcgaccgca cccccggcac cctctccccc gcccaactgc cctgggtgcg tgcggcgagt
     1321 ggggtggtcg tccgcgaggt cgaggccgcg gccccggcac cgccgcccct ggcactccac
     1381 gagtcgccgc ggctgcgtgc gctcaggttc gatcgcagca tcgacgccga ctggcgacgc
     1441 acgtcgtact cgggcctgac cgcgggggtg cacgccgacc tgccgctgct ggccgacgaa
     1501 cccgacgacg ccgagtccgt ggttgcgccg gccggctcgt cggtgccctc cccgatgcgc
     1561 gacctgcccg ggggcacgca gttcggctcg ctggtgcacc agcttttcga gcacgtcgac
     1621 ccggggtcgg acgacaccac cctgaccgcg cggctgcggg ccgccgctgc tgaatggctg
     1681 ccacggtttc cgctgtccgg ggtcacctcc caggccctcg cggacgccct gttgccggcg
     1741 tttcacaccc cgctcggtcc gctggccgat gaccgcagcc tggcgcagtt gccgatcacc
     1801 gaccggctgg ccgagctcag cttcgaccta cccatggcgc cggcgtctgg gttcacgctc
     1861 ggcgacgtgg cccgcttgct gcgtgagcat ctgcccaccg gcgatccgct ggtcggctac
     1921 cccgcgctac tggacgctcc ggccctggcc gggcagccgc tgaaggggtt tctgaccggc
     1981 agtatcgatg cggtgctgcg ggtgggcacg cctgagcatc cgcggttcgt ggtcgtcgac
     2041 tacaagacca accggctcgg cggcgacgac ctgacgctgg gtcactacgc gcacgcgccg
     2101 ctggtggccg ccatgtgcgc ctcgcactac ccgctgcagg cgttgctgta ctgcgtcgcc
     2161 ctgcaccgct ttctggccgc ccgtctggtg cgttacgacc cgcatcggca cctgggcggg
     2221 gtgttgtatc tgttcgtccg aggcatgggg ggcgcgacgg ccgggccaag gaccggcgtg
     2281 ttcgcctggc aaccgcccgt cacgctgatc gaacggctgt cccgggtgtt gggaggtttc
     2341 agcgatgact gatccggcac tgtccgtggc gacctcgggg ttgaccggcg agttccacat
     2401 cctcggggtg ctggactggg cagacctgca ccccgctcga cagttcacca ggctgtacca
     2461 cgagcccgac gaacgggtgc aactggccgc cgcgctcacc gtccgggcat tgcgggccgg
     2521 atcggtctgc ctcgagctcg accgcgtacg caccggcgtc accggccgtg acgacgagct
     2581 ggtcgacgtg cccgacgagc tctggcccga ccccgacgac tggctgcgcg cgctgcaggc
     2641 aagtcctctg gtcgcccagg gccccggcgg tgacgacacg ctgccgctac ggctgatcga
     2701 tcacaggctg tacctgaccc gctactggca gcaggaggag caggtgcggc gcagcctgca
     2761 gcagcgctcg tcggcgcatg cgggcgtggc ggcgcctgag cagctggcgg cgtggctcga
     2821 cgagctcttt ccggggccga acgccgaccc cgaccagcgc cagggcgtgg cgcaggtgac
     2881 cacctcgggt gtcaccgtgg tggccggcgg acccggcacc ggcaagacca ccacgatcgc
     2941 ccgcatgatc gcggtgctgc tgcggcaggg cgtccagcgg gtggcgttgg cggcacccac
     3001 cggcaaggcc gcggcccgca tggacgaggc gctgcgcgcc gccgcacacg acctgccggc
     3061 cgatgtcgcc gaccgggtgg cgcaggtaag ggccagcacc ctgcatcggt tgctgggtgc
     3121 ccagccgtac cgcaacccgc cgtttcgcca ccatcgcgac aacccgctgc cccacgacgt
     3181 ggtgatcgtc gacgagctca gcatggtgtc gttgacgatg atggcccaac tcaccgacgc
     3241 gctgggtgtg cacacccggc tggtgctggt gggcgacccg gaccagctga gttcggtggg
     3301 tgccggtgcc gtgctcggcg acctgacctc ggcggggtgg cagggcaccg gcgtacacaa
     3361 cccggtggtg cggctgaggc gcaactaccg gttcgccggc gtgctgggcg aactggccga
     3421 ggcggtgcga acaggcgacg ccgagaccgt gctgcgcctt gtcgatgggg ccgaagcgaa
     3481 cctcaccctg accggcccgg acgatcccca gcacgcactg cgcgcccggg tcgtccacca
     3541 gggccggg
//
LOCUS       DAOSUG010000327         3540 bp    DNA     linear   ENV 10-JUN-2024
DEFINITION  MAG TPA_asm: Micropruina sp. isolate AMR_MDS_2195
            NODE_10832_length_3540_cov_3.284075, whole genome shotgun sequence.
ACCESSION   DAOSUG010000327 DAOSUG010000000
VERSION     DAOSUG010000327.1
DBLINK      BioProject: PRJNA925477
            BioSample: SAMN32864262
            Sequence Read Archive: SRR4236673
KEYWORDS    WGS; ENV; Metagenome Assembled Genome; MAG; Third Party Data; TPA;
            TPA:assembly.
SOURCE      Micropruina sp. (wastewater metagenome)
  ORGANISM  Micropruina sp.
            Bacteria; Bacillati; Actinomycetota; Actinomycetes;
            Propionibacteriales; Nocardioidaceae; Micropruina.
REFERENCE   1  (bases 1 to 3540)
  AUTHORS   Abdulkadir,N., Saraiva,J.P., Zhang,J., Stolte,S., Gillor,O.,
            Harms,H. and Rocha,U.
  TITLE     Genome-centric analyses of 165 metagenomes show that mobile genetic
            elements are crucial for the transmission of antimicrobial
            resistance genes to pathogens in activated sludge and wastewater
  JOURNAL   Microbiol Spectr 12 (3), e0291823 (2024)
   PUBMED   38289113
REFERENCE   2  (bases 1 to 3540)
  AUTHORS   Abdulkadir,N.
  TITLE     Direct Submission
  JOURNAL   Submitted (24-MAR-2023) Environmental Microbiology, Helmholtz
            Center for Environmental Research -UFZ, Permoserstr 15, Leipzig
            04318, Germany
COMMENT     The annotation was added by the NCBI Prokaryotic Genome Annotation
            Pipeline (PGAP). Information about PGAP can be found here:
            https://www.ncbi.nlm.nih.gov/genome/annotation_prok/
            
            ##Genome-Assembly-Data-START##
            Assembly Method        :: SPAdes v. 3.11.1
            Genome Representation  :: Full
            Expected Final Version :: Yes
            Genome Coverage        :: Not Applicable
            Sequencing Technology  :: Illumina MiSeq
            ##Genome-Assembly-Data-END##
            
            ##Genome-Annotation-Data-START##
            Annotation Provider               :: NCBI
            Annotation Date                   :: 05/04/2023 04:57:10
            Annotation Pipeline               :: NCBI Prokaryotic Genome
                                                 Annotation Pipeline (PGAP)
            Annotation Method                 :: Best-placed reference protein
                                                 set; GeneMarkS-2+
            Annotation Software revision      :: 6.5
            Features Annotated                :: Gene; CDS; rRNA; tRNA; ncRNA
            Genes (total)                     :: 2,776
            CDSs (total)                      :: 2,750
            Genes (coding)                    :: 2,714
            CDSs (with protein)               :: 2,714
            Genes (RNA)                       :: 26
            tRNAs                             :: 24
            ncRNAs                            :: 2
            Pseudo Genes (total)              :: 36
            CDSs (without protein)            :: 36
            Pseudo Genes (ambiguous residues) :: 0 of 36
            Pseudo Genes (frameshifted)       :: 2 of 36
            Pseudo Genes (incomplete)         :: 32 of 36
            Pseudo Genes (internal stop)      :: 2 of 36
            ##Genome-Annotation-Data-END##
FEATURES             Location/Qualifiers
     source          1..3540
                     /organism="Micropruina sp."
                     /mol_type="genomic DNA"
                     /submitter_seqid="NODE_10832_length_3540_cov_3.284075"
                     /isolate="AMR_MDS_2195"
                     /isolation_source="Activated sludge of municipal
                     wastewater treatment plants"
                     /db_xref="taxon:2737536"
                     /environmental_sample
                     /geo_loc_name="Canada"
                     /lat_lon="49.25 N 123.1 W"
                     /collection_date="2015-06-16"
                     /metagenome_source="wastewater metagenome"
                     /note="metagenomic"
     gene            complement(<1..420)
                     /locus_tag="PLF56_10410"
     CDS             complement(<1..420)
                     /locus_tag="PLF56_10410"
                     /EC_number="2.1.-.-"
                     /inference="COORDINATES: protein motif:HMM:NF024880.2"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_function: GO:0008168 - methyltransferase activity
                     [Evidence IEA];
                     GO_function: GO:1904047 - S-adenosyl-L-methionine binding
                     [Evidence IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="class I SAM-dependent methyltransferase"
                     /protein_id="HOQ54012.1"
                     /translation="MTKYDARWFASQAATWNDDPRKVERARQAADAIAAEVPLDPSWR
                     VLDYGAGTGLLAFALADRVGEILLLDFSDGMVEAARRRIAESGRPGMRAERHDLTQAP
                     LPATEQFDLVISMMVLHHVDDVPLLLTRLSAATTEDGW"
     gene            complement(498..1034)
                     /locus_tag="PLF56_10415"
     CDS             complement(498..1034)
                     /locus_tag="PLF56_10415"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_017935533.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="CGNR zinc finger domain-containing protein"
                     /protein_id="HOQ54013.1"
                     /translation="MDFTHDTRLSLQALAALVNTRDPDTLTTVDQLDELCRRWQWSGL
                     RTGDQAELQSVRDVRDRLAGFWGADEHAVVELINALLVEGRALPQLVKHDDFDWHIHA
                     TRPGAPLVVRMIVEYAMSMMDVVRAGDLDRLHYCAAPDCDFVVIDLSRNRSRRFCDSG
                     CGNRANVAAYRARRRATG"
     gene            1141..2085
                     /locus_tag="PLF56_10420"
     CDS             1141..2085
                     /locus_tag="PLF56_10420"
                     /inference="COORDINATES: protein motif:HMM:NF013088.2"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_function: GO:0005215 - transporter activity [Evidence
                     IEA];
                     GO_process: GO:0055085 - transmembrane transport [Evidence
                     IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="DMT family transporter"
                     /protein_id="HOQ54014.1"
                     /translation="MTTTPVNAGREALVGWTAALVSAAAFGTSGAFAKPLLATGWSAG
                     AVVAIRLGLAAAVLLVPGLVALRGRWRVLLEHWRPIACYGVFGGATAQVAFFNSVQYV
                     PVALALLLEYLGILLVVAWVALRSRRLPTRLTLVGVAVSIGGLVIVLDPSGMAGLDWR
                     GIGWGLLAAFGLAVYFVVSARPMTLPPLTFVAVGLSVGTVAIVLAGLSGLMPLHAAVA
                     DVRFVGITLAWWAPLLELALVAAAAAYALGFVGVQHLGSTVASFVGLTEVLFAVLWAW
                     LLLGELPGLLQLVGGVILLAGVAAVQRGQARETSARTQ"
     gene            complement(2388..>3540)
                     /locus_tag="PLF56_10425"
     CDS             complement(2388..>3540)
                     /locus_tag="PLF56_10425"
                     /EC_number="6.3.4.4"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_002525476.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_function: GO:0004019 - adenylosuccinate synthase
                     activity [Evidence IEA];
                     GO_function: GO:0005525 - GTP binding [Evidence IEA];
                     GO_process: GO:0006164 - purine nucleotide biosynthetic
                     process [Evidence IEA]"
                     /codon_start=2
                     /transl_table=11
                     /product="adenylosuccinate synthase"
                     /protein_id="HOQ54015.1"
                     /translation="NGEKYALHLLPSGILSPKSIPVIGNGVVIDLDVLFDEIDGLAAR
                     GVDAATRLLVSSNAHLITPYHQTIDKVSERFLGKRKIGTTGRGVGPAYSDKVNRLGIR
                     VQDLFDEEMLRQKVEAALWQKNHLLVKVYNQRAIDPNEIADRLLSHAERLRPMVADTS
                     RVLNDALNADQVVLFEGAQAHHLDVDHGTYPYVTSSNPIAAGACTGAGVGPTRIDRVV
                     GIAKAYTTRVGEGPFPTELLDADGDRIRADGAEFGTTTGRKRRCGWYDALVVEAAASA
                     NAFTDIFLTKLDILSGWEQIPVCVAYDVDGVRHDHLPMTQHDFARATPIYELQEGWSE
                     DISGARSFGELPTTCQAYVRRLEELSGARISGIGVGPGREQVVMLHDLL"
BASE COUNT          508 a         1146 c         1265 g          621 t
ORIGIN      
        1 ccagccgtct tcggtggtcg ccgccgacaa gcgcgtgagc agcagcggta cgtcatcgac
       61 gtggtgcagc accatcatgc tgatcaccag gtcgaactgc tcggtcgcgg gcagcggcgc
      121 ctgcgtgagg tcgtgtcgct cggcccgcat gccgggccgt ccggactcgg cgatgcgccg
      181 acgggcggcc tcgaccatgc cgtccgagaa atcgagcagc aggatctcgc ccacccggtc
      241 ggccagcgcg aacgcgagca gtccggtgcc ggcgccatag tcgagcaccc gccaggacgg
      301 gtcgaggggc acctcggcag caatcgcatc tgcggcctgg cgggcccgct caaccttgcg
      361 cggatcgtcg ttccaggtcg ccgcctggct ggcgaaccag cgggcgtcgt acttcgtcat
      421 acggctcatg ctgccccagt ggggctcggc tgggaagcga ttacgcaacc taaggtggtc
      481 ggcggggctg cggccggtca gccagtggcc cgacggcggg cccgataggc ggccacgttg
      541 gcgcggttac cacagcccga atcacagaat cgccgcgaac gattgcggct caggtcgatc
      601 acgacgaagt cgcagtcggg tgcggcgcag tagtgcagcc ggtcgaggtc gccggcgcga
      661 accacgtcca tcatcgacat cgcgtactcg acgatcatgc ggacgaccag gggcgcgccc
      721 gggcgggtgg cgtgaatgtg ccagtcgaag tcgtcgtgct tgacgagttg tggcagcgcg
      781 cggccttcga ccagaagcgc gttgatcaac tcgaccaccg cgtgctcgtc cgcgccccag
      841 aagccggcca accggtctcg aacgtctcgg acgctctgca actcggcctg gtcacccgtg
      901 cgcaagcccg accactgcca ccggcggcac agttcgtcca actggtcgac ggtggtcagg
      961 gtgtcggggt cgcgggtgtt caccagggcc gccagggcct ggagtgacaa tcgggtgtca
     1021 tgagtgaaat ccatgttgac tcctgacccc ggtctgcgat gtcatgatcg tgagtcgttg
     1081 tgactcatga cattgagtgt aggcacgagg ggacgtccga tggctgaagc ggtggttgag
     1141 gtgactacca ccccggtgaa cgccggacgc gaggcgctgg tcggctggac ggccgccctc
     1201 gtcagcgcag ccgccttcgg cacctcgggc gcgttcgcca aaccgctgtt ggcaacgggc
     1261 tggagcgcgg gagccgtggt cgccatccgg ctcggcctgg cggccgcggt actactggtt
     1321 cccggtctgg tggccctgcg cggacggtgg cgggtgctgc tggagcactg gcgtccgatc
     1381 gcgtgttacg gagtgttcgg cggcgcgacg gcgcaggtgg ccttcttcaa ctcggtgcag
     1441 tacgtgccgg tggcgttggc cctgctgctg gagtacctgg gcattctgct cgtggtggca
     1501 tgggtggccc tgcgcagccg gcggttgccg acccggctca cgctggtggg ggtcgccgtg
     1561 tccatcggcg ggttggtgat cgttctcgac ccgtccggca tggccggcct ggattggcgg
     1621 ggcatcggct ggggactgtt ggcggcgttc gggcttgcgg tgtatttcgt ggtgtccgca
     1681 cggccgatga cgttgccgcc gctcactttc gtggccgtcg gcctgagcgt cgggaccgtg
     1741 gccattgtgc tggccgggct gagcggtctg atgcccctgc acgccgccgt cgcagacgtc
     1801 cgtttcgtcg ggatcacact ggcgtggtgg gcaccgctac tcgagctcgc gttggtagcc
     1861 gccgccgcgg cctacgcgct gggtttcgtg ggagtgcagc acctgggctc gacggtggcg
     1921 agctttgtcg gcctgaccga ggtgttgttc gccgtgttgt gggcctggct gctgctcggc
     1981 gagctgcctg ggctgctgca gctcgtgggc ggggtgatcc tgctggccgg cgtggccgcc
     2041 gttcagcgcg gacaggcgcg cgagacctca gcccgaaccc agtaggcggt tcgaggacgg
     2101 ttgagccggt ttggcccgga tccaccgatg gccggcgtcg cgagcggcac cagacgggtg
     2161 gctgcaagcc ggtccgcgcg aacgcagcct gggcatactt tgagcgtgcc cgacgaagcc
     2221 ggaccacgga tggggctgcg cggcagacgc cgatcggcgg atacggttca gggaagtgct
     2281 gatcgaagag cgaaccgttg tcgagggtcg aaactcctgt cgcaccggtc tcgacgcttg
     2341 accagcgatc gatcagcgcg tcccttgcta ccgtccccga cctgcactca gagcaggtcg
     2401 tgcagcatga cgacctgctc gcgacccgga cccacaccga tgcccgagat gcgggcaccg
     2461 ctcagttcct ccagccggcg gacgtacgcc tggcaggtcg tcggcagctc gccgaaggac
     2521 cgcgcacctg agatgtcttc gctccagcct tcctgcagct catagatcgg cgtggcccgc
     2581 gcgaagtcgt gctgggtcat cggcaggtgg tcgtgccgca caccgtccac gtcgtaggcg
     2641 acgcagaccg ggatctgctc ccaccccgac aagatgtcga gcttggtcaa gaagatatcg
     2701 gtgaacgcgt tcgcgctcgc cgcggcctca acgaccaggg cgtcgtacca gccacaacga
     2761 cgcttgcgtc cggtggtcgt gccgaactcg gcaccgtcgg cgcggatccg gtcgccgtcg
     2821 gcgtccagca gctcggtcgg gaacggtccc tcgcccaccc gtgtggtgta ggccttggcg
     2881 atgccgacca cccggtcgat gcgggtcggg ccgaccccgg cgccggtgca ggcgcccgcc
     2941 gcgatcgggt tggacgaggt gacgtagggg taggtgccgt ggtcgacgtc gaggtggtgc
     3001 gcctgggcac cctcgaacaa caccacctgg tcggcgttca gggcgtcgtt gagcacccgg
     3061 ctggtgtcgg ccaccatcgg acgcagccgc tcggcatggc tgagcagccg atcagcgatc
     3121 tcgttggggt cgatcgcgcg ctggttgtac accttcacca gcaggtgatt cttctgccac
     3181 agggccgcct cgaccttctg gcgcagcatc tcttcatcga acaggtcctg cacccgaatg
     3241 ccgaggcgat tgaccttgtc ggagtaggcc gggccgacac cgcgtccggt ggtgccgatc
     3301 tttcgcttgc cgagaaagcg ctccgacacc ttgtcgatcg tctggtgata gggcgtgatc
     3361 agatgcgcgt tggacgacac cagcaggcgc gtggcagcgt ccacaccgcg ggcggcgagt
     3421 ccgtcgatct cgtcgaagag cacgtcgagg tcgatcacca cgccgttgcc gatcaccggg
     3481 atgctcttcg ggctcaggat tcctgaaggg agcaggtgca gcgcgtactt ctcaccgttc
//
LOCUS       DAOSUG010000328         3529 bp    DNA     linear   ENV 10-JUN-2024
DEFINITION  MAG TPA_asm: Micropruina sp. isolate AMR_MDS_2195
            NODE_10869_length_3529_cov_4.676742, whole genome shotgun sequence.
ACCESSION   DAOSUG010000328 DAOSUG010000000
VERSION     DAOSUG010000328.1
DBLINK      BioProject: PRJNA925477
            BioSample: SAMN32864262
            Sequence Read Archive: SRR4236673
KEYWORDS    WGS; ENV; Metagenome Assembled Genome; MAG; Third Party Data; TPA;
            TPA:assembly.
SOURCE      Micropruina sp. (wastewater metagenome)
  ORGANISM  Micropruina sp.
            Bacteria; Bacillati; Actinomycetota; Actinomycetes;
            Propionibacteriales; Nocardioidaceae; Micropruina.
REFERENCE   1  (bases 1 to 3529)
  AUTHORS   Abdulkadir,N., Saraiva,J.P., Zhang,J., Stolte,S., Gillor,O.,
            Harms,H. and Rocha,U.
  TITLE     Genome-centric analyses of 165 metagenomes show that mobile genetic
            elements are crucial for the transmission of antimicrobial
            resistance genes to pathogens in activated sludge and wastewater
  JOURNAL   Microbiol Spectr 12 (3), e0291823 (2024)
   PUBMED   38289113
REFERENCE   2  (bases 1 to 3529)
  AUTHORS   Abdulkadir,N.
  TITLE     Direct Submission
  JOURNAL   Submitted (24-MAR-2023) Environmental Microbiology, Helmholtz
            Center for Environmental Research -UFZ, Permoserstr 15, Leipzig
            04318, Germany
COMMENT     The annotation was added by the NCBI Prokaryotic Genome Annotation
            Pipeline (PGAP). Information about PGAP can be found here:
            https://www.ncbi.nlm.nih.gov/genome/annotation_prok/
            
            ##Genome-Assembly-Data-START##
            Assembly Method        :: SPAdes v. 3.11.1
            Genome Representation  :: Full
            Expected Final Version :: Yes
            Genome Coverage        :: Not Applicable
            Sequencing Technology  :: Illumina MiSeq
            ##Genome-Assembly-Data-END##
            
            ##Genome-Annotation-Data-START##
            Annotation Provider               :: NCBI
            Annotation Date                   :: 05/04/2023 04:57:10
            Annotation Pipeline               :: NCBI Prokaryotic Genome
                                                 Annotation Pipeline (PGAP)
            Annotation Method                 :: Best-placed reference protein
                                                 set; GeneMarkS-2+
            Annotation Software revision      :: 6.5
            Features Annotated                :: Gene; CDS; rRNA; tRNA; ncRNA
            Genes (total)                     :: 2,776
            CDSs (total)                      :: 2,750
            Genes (coding)                    :: 2,714
            CDSs (with protein)               :: 2,714
            Genes (RNA)                       :: 26
            tRNAs                             :: 24
            ncRNAs                            :: 2
            Pseudo Genes (total)              :: 36
            CDSs (without protein)            :: 36
            Pseudo Genes (ambiguous residues) :: 0 of 36
            Pseudo Genes (frameshifted)       :: 2 of 36
            Pseudo Genes (incomplete)         :: 32 of 36
            Pseudo Genes (internal stop)      :: 2 of 36
            ##Genome-Annotation-Data-END##
FEATURES             Location/Qualifiers
     source          1..3529
                     /organism="Micropruina sp."
                     /mol_type="genomic DNA"
                     /submitter_seqid="NODE_10869_length_3529_cov_4.676742"
                     /isolate="AMR_MDS_2195"
                     /isolation_source="Activated sludge of municipal
                     wastewater treatment plants"
                     /db_xref="taxon:2737536"
                     /environmental_sample
                     /geo_loc_name="Canada"
                     /lat_lon="49.25 N 123.1 W"
                     /collection_date="2015-06-16"
                     /metagenome_source="wastewater metagenome"
                     /note="metagenomic"
     gene            complement(447..869)
                     /locus_tag="PLF56_10430"
     CDS             complement(447..869)
                     /locus_tag="PLF56_10430"
                     /inference="COORDINATES: ab initio
                     prediction:GeneMarkS-2+"
                     /note="Derived by automated computational analysis using
                     gene prediction method: GeneMarkS-2+."
                     /codon_start=1
                     /transl_table=11
                     /product="hypothetical protein"
                     /protein_id="HOQ54016.1"
                     /translation="MNYQYRRELLESATGALHADEQGLVSALPTELGHAFVLITCHDR
                     IDQPAAALAEALGCLASARSSSPMARVLHGQRLPFLLNLVNDLSSRPHGWIRDALTAV
                     GAAAASRTTAEARAQIVVAERAIRRGIGEVTQQIVSAA"
     gene            1427..2029
                     /locus_tag="PLF56_10435"
     CDS             1427..2029
                     /locus_tag="PLF56_10435"
                     /inference="COORDINATES: protein motif:HMM:NF024212.2"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_function: GO:0003700 - DNA-binding transcription factor
                     activity [Evidence IEA];
                     GO_process: GO:0006355 - regulation of DNA-templated
                     transcription [Evidence IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="MarR family winged helix-turn-helix
                     transcriptional regulator"
                     /protein_id="HOQ54017.1"
                     /translation="MTSSDRAQLLRTLAELVRGVGWASERVGHSFASSQGLHPTDFRA
                     LTAIYAADLGGAPLTVRELAQRLDLTPAAITYAVDRLTAAGHVWRERGTHDRRTVVLR
                     FGEHGHQVARDFFTPLGDLHTQALEDFSDTDLAACVRVLGVVNQALARFDSDLRNPDP
                     STQPTSTSGAEGAQSSDTAQTSDTAQTSDTAQPSETEEQR"
     gene            2026..>3529
                     /locus_tag="PLF56_10440"
     CDS             2026..>3529
                     /locus_tag="PLF56_10440"
                     /inference="COORDINATES: protein motif:HMM:NF019310.2"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="MFS transporter"
                     /protein_id="HOQ54018.1"
                     /translation="MSARRSRWAFAMGLYLLGIFMGAIDTGIVTPARTIIQNDLGVDD
                     QLGIWMITIYTLSYAAAIPVMGKLADRLGRKPVYLTAIALFGIGSLLCGLSQDVGSFG
                     MLIAARSLQAIGGGGILPIATAEIGTEVPEERRGLALGLVGAVFGIANLLGASAGSLI
                     LDIAGQHNWQWIFYINVPIAAMIVIAGWVVLPNHRESRVAKLDLVGIAILVAMIMSLL
                     YGLTNIDFFDLSASVTSLEVYPFLIAFVVLLPVFVLAERRAEDPVLNLDYFTNRPIAL
                     TLAASFLSGFILMGVVFVPQLAENALELPSGSGGYLVILLGLASGVGAPLSGRLTDAF
                     GPKLVLAIGGGVSALAAVSVVTWLIPHPGYPSVIVSLVLIGLGLGFVIGSPLNYLMLR
                     LTDPAQSNSALGTLSLVRSIGTTLAPVIMVGFLAHAGGLVQDRLTADLPSTIKAPELP
                     YAASLQKKFAAWKADDRFSDALKGVEFPDLSSKTTIEVNTDGGGELPDELV"
BASE COUNT          547 a         1246 c         1145 g          591 t
ORIGIN      
        1 gcgaacggca gacggcggca ccgcgacgac gcccaagcct ctgccacgtc aaaacccttt
       61 cttgtgctgg gcgcgaagcc cgttgctgtc gggtgtcaga ggcttgggcg tcgtcgcggt
      121 gccgccgtct gccgttcgca ctctactggt gagagtgcta acgcgaatct atcgcggcat
      181 tagcactcgt tcaacccgag tgccaacgaa agtgatggac ggcgcccgtg atcgatccgt
      241 gggagcccgc cgatgcgggc tcctgtgaca cgcgccaccg ggacgtcgcc accctcctgg
      301 cacccgactc ggaggtagct gccctcctga cacccgactc ggacgtagct gccctcccgg
      361 cacccgactc ggacgtagct gccctcccgg cacccgactc ggaggtagct ggtcacccgg
      421 ctactagcgg acgtccgcca aaggactcag gcggcgctca cgatctgctg ggtcacctca
      481 ccgatgccgc gccggatggc ccgctcggcg accacgatct gagcccgcgc ctcggccgtg
      541 gtgcgtgagg ctgcggccgc gccgacggcg gtgagagcgt cgcggatcca gccgtgcgga
      601 cgagaggaca ggtcgttcac cagattgagc agaaacggca gccgctgccc atggaggacc
      661 cgcgccatcg ggctggacga gcgcgctgac gccaggcagc ccaacgcctc ggcgagggca
      721 gcggcgggct ggtcgatccg gtcgtgacag gtaatcagca cgaaggcgtg gcccagttcg
      781 gtggggagcg cgctgacgag cccctgctcg tcggcatgaa gcgcccccgt ggcgctctct
      841 agcagctctc tgcgatattg gtagttcata ccaaaaagtc tagtggacgt tccgtggcaa
      901 gcacgaccgc gaaacccaat gttcacctga tcaccccgcc gcgttggtcc gaccctgtcc
      961 accccctgcc ccgacgtcgc ctccccacgc tcgggccctg acctcactgc gtccatcaca
     1021 ccttgcggga gcgcagatcg atgcgcgaca ccatggtcga acgtcgagac ccctggtcgc
     1081 acgagtctcg acacgcgcga ccgtccgatc gcggattgct cagcccggat ccaccgatct
     1141 gcgcccactg caaccggccc acgcaacgcc cccccaacta tctccggccc gcgtagcttc
     1201 gcgggccagt gatggttagg ggggcgcatg gcgcgccctg gcgtcgccgg ctacgcgggg
     1261 caggcacccg gcatgcccac gcgcggacgt cgagacccct ggtcgcccgg ctctcgacac
     1321 gcgcgaccgt ccggtcgcgg atagctcagt tcctgcttac cctccgcaaa tactctcagc
     1381 cacttgatcg tcaaatggtt taactatgtc ggctatgctc tggtccgtga cttcatccga
     1441 tcgagcccag ctgctgcgca ccctcgccga actcgtccgc ggtgtcggct gggcgtcgga
     1501 gcgggtcggg cacagcttcg cgtccagcca ggggttgcac ccgactgact tccgcgcgct
     1561 caccgcgatc tatgccgccg acctgggcgg cgcaccactg acggtgcgcg aactggcgca
     1621 gcggctcgac ctgacccccg ccgccatcac ctatgccgtc gaccggctga ccgccgccgg
     1681 gcacgtctgg cgcgaacgcg gcacccacga ccggcgcacg gtggtgctgc gcttcggtga
     1741 gcacggtcac caggtggccc gcgacttctt caccccgctc ggcgacctgc acacccaggc
     1801 gctggaggac ttctccgaca ccgacctggc cgcctgcgtc cgggtgctgg gcgtggtgaa
     1861 tcaggcactg gcgcggttcg acagcgacct gcgcaacccc gacccgtcca cccagccaac
     1921 gtccacgtcc ggcgccgaag gggcgcagtc gagcgacacc gcgcagacga gcgacaccgc
     1981 gcagacgagc gacaccgcgc agccgagcga gaccgaggag cagcgatgag cgcccggcgt
     2041 tccaggtggg ccttcgcgat ggggctctac ctgctcggca tcttcatggg ggcgatcgac
     2101 accggcatcg tcacgccggc ccgcaccatc attcagaacg acctcggcgt cgacgaccag
     2161 ctcggcatct ggatgatcac catctacacc ctgtcgtacg cggcggcgat cccggtgatg
     2221 ggcaagctgg ccgaccggct gggccgcaag ccggtctacc tgaccgccat cgccctgttc
     2281 ggcatcggct cgctgctgtg cggcctgtcg caggacgtgg gcagcttcgg catgctgatc
     2341 gccgcgcgct cactgcaggc catcggcggc gggggcattc tgccgatcgc gaccgccgag
     2401 atcggcaccg aggtgccgga agaacgccgt ggcttggcgc tcggcctggt gggagccgtg
     2461 ttcggtatcg cgaacctgct gggagcgtcc gccggtagcc tgattctcga catcgccggt
     2521 cagcacaact ggcaatggat cttctacatc aacgtgccga tcgccgcgat gatcgtgatc
     2581 gccgggtggg tggtgctgcc caaccaccgc gagagccgcg tggcgaagct cgacctggtc
     2641 ggcatcgcga tcctggtcgc aatgatcatg agcctgctgt acgggctgac caacatcgac
     2701 ttcttcgacc tgtcggccag cgtcacctcg ctggaggtat acccgttcct gatcgccttc
     2761 gtagtgctgt tgccggtgtt cgtgctggcc gagcggcgag cggaagaccc ggtgttgaac
     2821 ctggactact tcaccaaccg tccgatcgcg ctgaccctgg cggcttcgtt tctgtcgggc
     2881 ttcattttga tgggcgtggt gttcgtgccg cagctcgccg agaacgcgct cgagttgccc
     2941 agcggcagcg gcggctacct ggtgattctg ctggggttgg cctcgggcgt gggtgccccc
     3001 ttgtccggac ggttgacgga cgccttcggc ccgaagctcg tgttggcgat cggaggcggc
     3061 gtgtctgcgt tggccgcggt cagcgtggtc acctggctga tcccgcaccc cggctacccg
     3121 agcgtgatcg tgagcctggt gctgatcggc cttggcctgg ggttcgtgat cggctcaccg
     3181 ctcaactacc tgatgttgcg gctgacggat cccgcgcagt cgaactcggc actgggcacc
     3241 ctgtcactgg tgcgatccat cggcaccacg ctcgcgccgg tgatcatggt cggctttctc
     3301 gctcacgccg gcggcctggt gcaggaccgg ctgaccgccg acctaccgtc caccatcaag
     3361 gcgcccgaac taccgtatgc ggcctcgctg cagaagaagt tcgcagcctg gaaggcggac
     3421 gaccggttct ccgacgccct gaagggcgtg gagtttcccg acctgagcag caagaccacg
     3481 atcgaggtga acaccgacgg cggcggcgaa ctgcccgacg agctggtca
//
LOCUS       DAOSUG010000329         3525 bp    DNA     linear   ENV 10-JUN-2024
DEFINITION  MAG TPA_asm: Micropruina sp. isolate AMR_MDS_2195
            NODE_10882_length_3525_cov_4.208646, whole genome shotgun sequence.
ACCESSION   DAOSUG010000329 DAOSUG010000000
VERSION     DAOSUG010000329.1
DBLINK      BioProject: PRJNA925477
            BioSample: SAMN32864262
            Sequence Read Archive: SRR4236673
KEYWORDS    WGS; ENV; Metagenome Assembled Genome; MAG; Third Party Data; TPA;
            TPA:assembly.
SOURCE      Micropruina sp. (wastewater metagenome)
  ORGANISM  Micropruina sp.
            Bacteria; Bacillati; Actinomycetota; Actinomycetes;
            Propionibacteriales; Nocardioidaceae; Micropruina.
REFERENCE   1  (bases 1 to 3525)
  AUTHORS   Abdulkadir,N., Saraiva,J.P., Zhang,J., Stolte,S., Gillor,O.,
            Harms,H. and Rocha,U.
  TITLE     Genome-centric analyses of 165 metagenomes show that mobile genetic
            elements are crucial for the transmission of antimicrobial
            resistance genes to pathogens in activated sludge and wastewater
  JOURNAL   Microbiol Spectr 12 (3), e0291823 (2024)
   PUBMED   38289113
REFERENCE   2  (bases 1 to 3525)
  AUTHORS   Abdulkadir,N.
  TITLE     Direct Submission
  JOURNAL   Submitted (24-MAR-2023) Environmental Microbiology, Helmholtz
            Center for Environmental Research -UFZ, Permoserstr 15, Leipzig
            04318, Germany
COMMENT     The annotation was added by the NCBI Prokaryotic Genome Annotation
            Pipeline (PGAP). Information about PGAP can be found here:
            https://www.ncbi.nlm.nih.gov/genome/annotation_prok/
            
            ##Genome-Assembly-Data-START##
            Assembly Method        :: SPAdes v. 3.11.1
            Genome Representation  :: Full
            Expected Final Version :: Yes
            Genome Coverage        :: Not Applicable
            Sequencing Technology  :: Illumina MiSeq
            ##Genome-Assembly-Data-END##
            
            ##Genome-Annotation-Data-START##
            Annotation Provider               :: NCBI
            Annotation Date                   :: 05/04/2023 04:57:10
            Annotation Pipeline               :: NCBI Prokaryotic Genome
                                                 Annotation Pipeline (PGAP)
            Annotation Method                 :: Best-placed reference protein
                                                 set; GeneMarkS-2+
            Annotation Software revision      :: 6.5
            Features Annotated                :: Gene; CDS; rRNA; tRNA; ncRNA
            Genes (total)                     :: 2,776
            CDSs (total)                      :: 2,750
            Genes (coding)                    :: 2,714
            CDSs (with protein)               :: 2,714
            Genes (RNA)                       :: 26
            tRNAs                             :: 24
            ncRNAs                            :: 2
            Pseudo Genes (total)              :: 36
            CDSs (without protein)            :: 36
            Pseudo Genes (ambiguous residues) :: 0 of 36
            Pseudo Genes (frameshifted)       :: 2 of 36
            Pseudo Genes (incomplete)         :: 32 of 36
            Pseudo Genes (internal stop)      :: 2 of 36
            ##Genome-Annotation-Data-END##
FEATURES             Location/Qualifiers
     source          1..3525
                     /organism="Micropruina sp."
                     /mol_type="genomic DNA"
                     /submitter_seqid="NODE_10882_length_3525_cov_4.208646"
                     /isolate="AMR_MDS_2195"
                     /isolation_source="Activated sludge of municipal
                     wastewater treatment plants"
                     /db_xref="taxon:2737536"
                     /environmental_sample
                     /geo_loc_name="Canada"
                     /lat_lon="49.25 N 123.1 W"
                     /collection_date="2015-06-16"
                     /metagenome_source="wastewater metagenome"
                     /note="metagenomic"
     gene            <1..637
                     /locus_tag="PLF56_10445"
     CDS             <1..637
                     /locus_tag="PLF56_10445"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_013601301.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=2
                     /transl_table=11
                     /product="hypothetical protein"
                     /protein_id="HOQ54019.1"
                     /translation="VRITCIAEERAQGLLCSVHQARRPHVSAFHDPERFADRDNPLTD
                     YHPAFHVTDPTSADTETDARTDADDNGSSGGGSDRACMSAGSAAEDAIALLCLLGWAP
                     ETARASVEHVCGALMRAGTRQSAYEGLRRGRHARVLLDLPGRSWTVLLRALLGSPHPA
                     YAATNAGKGVLLRLLIGETLPVLLRDDDLVLSLSLAAPTHRDRDRGGGDVL"
     gene            634..1659
                     /locus_tag="PLF56_10450"
     CDS             634..1659
                     /locus_tag="PLF56_10450"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_009882022.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="ParB N-terminal domain-containing protein"
                     /protein_id="HOQ54020.1"
                     /translation="MSEVQAGGVQLDRVVDSIIVGTRHRADLGDIDALAASIEREGLL
                     QPLTVTIDGVLVCGARRLAAIKKLRWRTVSVWVRSGLSDRLGQLLGEQDDNMLHKPLT
                     QLEAAALYRELKQEMAEDAARRQAATRFTSDHQPGSDGGGQCPPPSTGQLGKTREQAA
                     SMIGGGASYKTFERIGYLERIAADPAQPEPLRAQAAAALEQIEAGAPVHPIFQAVRDA
                     ATIAQDVREADLHQMADEAVARATAAKKQKDKTSAPRPVPLTDVDGEPVRYPVRAFIH
                     TWGELTQWWTHYDPDQLATELTDEQIEAFLDTAEGTSRFADELRAARDREPADDAPSG
                     RGHLRAL"
     gene            1775..2416
                     /locus_tag="PLF56_10455"
     CDS             1775..2416
                     /locus_tag="PLF56_10455"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_009882021.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="hypothetical protein"
                     /protein_id="HOQ54021.1"
                     /translation="MVIVVLAGIGVYGLLAGPPTSDRSPDRGGGTPSAPVVIAPGEPT
                     PTGTPSVPALPEVEASDDPETFARNVAETLFAWDTGSELMPLDYSSVVLAVGDPSGAE
                     QAGLASDVAAYLPTREAWLELRQYATAQHLTIEDAFVPEAWDTAVEQAQPGQLAEGTI
                     AYTVEGTRHRTGVWNDEQVTSEHPVAFTLFIVCAPTYDTCHLLRLSQLDNPLR"
     gene            2438..>3525
                     /locus_tag="PLF56_10460"
     CDS             2438..>3525
                     /locus_tag="PLF56_10460"
                     /EC_number="3.4.24.-"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_006213368.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_function: GO:0004222 - metalloendopeptidase activity
                     [Evidence IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="M23 family metallopeptidase"
                     /protein_id="HOQ54022.1"
                     /translation="MLKKVVVATLVLVFFGPVLALLSVGVLMNPAATATVACTVNSGV
                     TVGDVPDSLTVTTANGETFTLNRQQLTHAATIIETGSQIDGVTRDGLVIALMAALTES
                     TLRMLSNTSAYPESADYPNDGDGSDHDSLGLFQMRPQSGWGTVAELMDPIYQAQAFFG
                     GPTGPNHPSPRGLLDIPGWEQMDKGEAAQAVEVSAYPDRYRNYEPVAETILTTLTTTT
                     SAGAGGEVVQVAGPESSRVVFPVPEGTWVLTSEYGPRVHPITGEDSFHTGTDFAAPDG
                     TALLAAADGTVTVAEFSGGYGGLIVIEHTIGGQTVATAYAHMWEHGIHVQVGDTVTAG
                     EHIGDIGSSGNSTGPHLHFEVRLGGTDG"
BASE COUNT          570 a         1283 c         1179 g          493 t
ORIGIN      
        1 ggtgcggatc acctgcatcg ccgaggaacg cgcccaagga ctgctctgct cggtccacca
       61 agcccgccgc ccgcacgtgt ccgcgttcca cgacccggag cggttcgccg accgggacaa
      121 cccgctcacc gactaccacc ccgccttcca cgtcaccgac cccacctcgg cggacaccga
      181 gaccgacgcc cgcaccgatg cggacgacaa cggcagttcc ggcggcgggt cggatcgggc
      241 gtgcatgtcc gcgggatcgg cggccgagga cgccatcgcc ttgctgtgcc tgctgggctg
      301 ggcgccggag accgcgcggg cctcggtcga gcacgtgtgc ggggcgctga tgcgggccgg
      361 cacccggcag tccgcgtatg aggggctgcg gcggggccgg cacgcccgcg tcctactgga
      421 cctgcccggc cgatcctgga ccgtgctgct gcgagcgctg ctcggcagcc cacaccccgc
      481 ctacgccgcc acgaacgccg ggaagggggt gctgctgcgg ctgctgatcg gcgagacgct
      541 accggtgctg ctacgcgacg acgacctggt gctctccctg tccctcgccg cccccaccca
      601 ccgtgaccgt gatcgtggcg ggggtgatgt gctgtgagtg aggtgcaggc cggcggagtc
      661 cagctcgacc gggtggtgga ctccatcatc gtcgggacac ggcaccgcgc cgacctgggc
      721 gacatcgacg ccctggccgc ctccatcgaa cgcgagggcc tgctgcaacc gctgacggtc
      781 accatcgacg gagtgctggt atgcggggcg cggcggttgg cggcgatcaa gaaactccgc
      841 tggcgcaccg tcagcgtctg ggttcgctcg ggcctgtcgg atcgtctcgg gcagttgctg
      901 ggcgagcaag acgacaacat gctgcacaag ccgctgaccc agctcgaagc cgccgccctg
      961 taccgggaac tgaaacagga gatggccgaa gacgccgccc ggcggcaggc cgcgacccgc
     1021 tttaccagcg accaccagcc cggaagcgac ggtggtggac aatgtccacc accgtcaacc
     1081 gggcaacttg gcaagacccg cgagcaggcc gcctccatga tcggcggcgg cgcctcctac
     1141 aagaccttcg agcggatcgg ctacctagaa cgcatcgccg ccgaccccgc ccaacccgaa
     1201 cccctgcgcg cgcaagctgc cgcggcgttg gagcagatcg aggccggcgc accagtgcac
     1261 ccgatcttcc aagccgtccg cgatgccgcg accatcgcgc aggacgttcg ggaggccgac
     1321 ctgcaccaga tggccgacga ggccgtcgcc cgcgccaccg ccgccaagaa gcagaaagac
     1381 aagacgtcgg cgccgcgtcc ggtgccgctg accgatgtgg atggggagcc ggtgcggtat
     1441 ccggtgcggg cgttcatcca cacctggggc gagctgaccc agtggtggac ccactacgac
     1501 ccagaccagc tcgccaccga gctgaccgac gagcagatcg aagccttcct cgacaccgcc
     1561 gaaggcacga gccggttcgc cgacgagctg cgcgccgccc gcgaccgcga accggccgac
     1621 gacgcgccct cgggccgggg gcacctgcgc gccctctgac cgacccgttc tgagtgcgag
     1681 ggaaggacca gcgcacctgt cgggtatgaa gactcccttc gctgcgtctg atgctcgtgc
     1741 ccggcgccgg ctcatcgccc tggtctctgc gctggtggtg atcgtggtgc tggccgggat
     1801 cggcgtctac ggcctcctgg ccgggccgcc caccagcgat cgcagccccg accgtggcgg
     1861 cggcacacca tcggctccgg tggtcattgc tcctggcgag ccgacgccga ccggaactcc
     1921 gtccgttccc gccttgccgg aggtcgaggc gtcggacgac ccagagacgt tcgcccgcaa
     1981 cgtcgccgag accttgttcg cgtgggacac cggcagcgag ttgatgccct tggattacag
     2041 ctcggtggtc ctcgctgtgg gtgatccttc gggtgcggag caggctgggc tcgcctctga
     2101 tgtcgcggcc tatctcccga ccagggaggc ctggctggag ctgcgtcagt acgcgaccgc
     2161 gcagcacctg accatcgagg acgccttcgt gccggaggcg tgggacacgg cggtggagca
     2221 ggcccaaccc ggacagctcg ccgaaggcac catcgcatac acggtcgagg gcacccgcca
     2281 ccgcaccggc gtctggaacg acgagcaggt gacctcggag catccggtgg cgttcacgct
     2341 cttcatcgtc tgcgcaccca cgtatgacac ctgccatctg ctgcgcctgt cccagctcga
     2401 caaccccctc cgatagccgc ctgggggtga gtggttcgtg ttgaagaagg tcgtcgtcgc
     2461 cacgctggtc ctggtgttct tcggacccgt cctggcgttg ttatcggtcg gggtcctgat
     2521 gaaccccgcc gccaccgcga ccgtcgcctg cacagtcaac agcggggtca cagtcggaga
     2581 cgtccccgac tcactgaccg tgacgaccgc gaacggtgag acgttcacgc tcaaccggca
     2641 acagctcacc cacgcggcga cgatcatcga gaccggcagc caaatcgacg gggtgacccg
     2701 cgacgggctg gtgatcgcgt tgatggcggc gcttaccgag tccaccctgc ggatgctctc
     2761 caacaccagc gcctaccccg agtccgcgga ctaccccaac gacggcgacg gctccgacca
     2821 cgactccctc ggcctgttcc agatgcgccc acaatccggg tggggcacgg tcgccgagct
     2881 gatggacccc atctaccagg cgcaggcgtt cttcggcgga cccaccggac ccaaccatcc
     2941 gagccccaga gggctgctgg acatccccgg ctgggagcag atggacaagg gcgaggccgc
     3001 ccaagccgtc gaagtgtccg cctaccccga ccggtaccgc aactacgagc cggtcgccga
     3061 gaccatcctc accacactca ccaccacgac gagcgcgggc gcgggcggcg aggtcgtaca
     3121 ggtggccggg ccagagtcct cgcgagtggt gttcccggtc ccggaaggca cctgggtcct
     3181 cacctccgaa tacgggccac gggtgcatcc catcaccgga gaggactcct tccacaccgg
     3241 gaccgacttc gccgccccgg acggcaccgc gctcttggcg gcagcggacg ggacggtgac
     3301 ggtggcggag ttctccggcg ggtacggcgg gctcatcgtc atcgagcaca ccatcggcgg
     3361 gcagacggtc gcgacggcgt atgcgcacat gtgggagcac ggcatccacg tccaggtcgg
     3421 cgacaccgtg acggcggggg agcacatcgg cgacatcggc tcctcgggca actccacggg
     3481 gcctcatctg catttcgagg tccgcctcgg cggcaccgac gggga
//
LOCUS       DAOSUG010000330         3522 bp    DNA     linear   ENV 10-JUN-2024
DEFINITION  MAG TPA_asm: Micropruina sp. isolate AMR_MDS_2195
            NODE_10898_length_3522_cov_3.349005, whole genome shotgun sequence.
ACCESSION   DAOSUG010000330 DAOSUG010000000
VERSION     DAOSUG010000330.1
DBLINK      BioProject: PRJNA925477
            BioSample: SAMN32864262
            Sequence Read Archive: SRR4236673
KEYWORDS    WGS; ENV; Metagenome Assembled Genome; MAG; Third Party Data; TPA;
            TPA:assembly.
SOURCE      Micropruina sp. (wastewater metagenome)
  ORGANISM  Micropruina sp.
            Bacteria; Bacillati; Actinomycetota; Actinomycetes;
            Propionibacteriales; Nocardioidaceae; Micropruina.
REFERENCE   1  (bases 1 to 3522)
  AUTHORS   Abdulkadir,N., Saraiva,J.P., Zhang,J., Stolte,S., Gillor,O.,
            Harms,H. and Rocha,U.
  TITLE     Genome-centric analyses of 165 metagenomes show that mobile genetic
            elements are crucial for the transmission of antimicrobial
            resistance genes to pathogens in activated sludge and wastewater
  JOURNAL   Microbiol Spectr 12 (3), e0291823 (2024)
   PUBMED   38289113
REFERENCE   2  (bases 1 to 3522)
  AUTHORS   Abdulkadir,N.
  TITLE     Direct Submission
  JOURNAL   Submitted (24-MAR-2023) Environmental Microbiology, Helmholtz
            Center for Environmental Research -UFZ, Permoserstr 15, Leipzig
            04318, Germany
COMMENT     The annotation was added by the NCBI Prokaryotic Genome Annotation
            Pipeline (PGAP). Information about PGAP can be found here:
            https://www.ncbi.nlm.nih.gov/genome/annotation_prok/
            
            ##Genome-Assembly-Data-START##
            Assembly Method        :: SPAdes v. 3.11.1
            Genome Representation  :: Full
            Expected Final Version :: Yes
            Genome Coverage        :: Not Applicable
            Sequencing Technology  :: Illumina MiSeq
            ##Genome-Assembly-Data-END##
            
            ##Genome-Annotation-Data-START##
            Annotation Provider               :: NCBI
            Annotation Date                   :: 05/04/2023 04:57:10
            Annotation Pipeline               :: NCBI Prokaryotic Genome
                                                 Annotation Pipeline (PGAP)
            Annotation Method                 :: Best-placed reference protein
                                                 set; GeneMarkS-2+
            Annotation Software revision      :: 6.5
            Features Annotated                :: Gene; CDS; rRNA; tRNA; ncRNA
            Genes (total)                     :: 2,776
            CDSs (total)                      :: 2,750
            Genes (coding)                    :: 2,714
            CDSs (with protein)               :: 2,714
            Genes (RNA)                       :: 26
            tRNAs                             :: 24
            ncRNAs                            :: 2
            Pseudo Genes (total)              :: 36
            CDSs (without protein)            :: 36
            Pseudo Genes (ambiguous residues) :: 0 of 36
            Pseudo Genes (frameshifted)       :: 2 of 36
            Pseudo Genes (incomplete)         :: 32 of 36
            Pseudo Genes (internal stop)      :: 2 of 36
            ##Genome-Annotation-Data-END##
FEATURES             Location/Qualifiers
     source          1..3522
                     /organism="Micropruina sp."
                     /mol_type="genomic DNA"
                     /submitter_seqid="NODE_10898_length_3522_cov_3.349005"
                     /isolate="AMR_MDS_2195"
                     /isolation_source="Activated sludge of municipal
                     wastewater treatment plants"
                     /db_xref="taxon:2737536"
                     /environmental_sample
                     /geo_loc_name="Canada"
                     /lat_lon="49.25 N 123.1 W"
                     /collection_date="2015-06-16"
                     /metagenome_source="wastewater metagenome"
                     /note="metagenomic"
     gene            complement(<1..1735)
                     /locus_tag="PLF56_10465"
     CDS             complement(<1..1735)
                     /locus_tag="PLF56_10465"
                     /inference="COORDINATES: protein motif:HMM:NF016944.2"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_function: GO:0004352 - glutamate dehydrogenase (NAD+)
                     activity [Evidence IEA];
                     GO_process: GO:0019551 - glutamate catabolic process to
                     2-oxoglutarate [Evidence IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="NAD-glutamate dehydrogenase"
                     /protein_id="HOQ54023.1"
                     /translation="MAAAIASRSDWINELNRCLSNQPKDSSDADLLPGFAERYVQHLP
                     EGELVSLDPAHVAELIRRHVRLGGVRRPGEAKISIVPPSDDRVSTLLLIVTDDRPFLV
                     DTVSMNITRSNWSIRALYHPQYEVLRDADGHLTAADADGRGEPESWICVELFPPLGHA
                     SQESSEQLADMVAAGLAQVEAAVDDWQPMQRRLAETVDWLGEHPQPVSPHGVQKVTDL
                     LEWLGRGNLVLLGYREYRREGDGYVSVPGTGLGILRDDNPGVSGFDAFPRPDDHHLLV
                     LTKDSRRSPVHRPAYIDYVGVRLYDAHGELQGERRFLGLLSAAAYSETVRKIPILAQK
                     AQQLMDRSGFDPRSHGGSAIRRVIATYPRDELFQASVDELFPVISQEAQFKDRRLVRV
                     FVRRDAYGRFASVVVYLPRDRYTTATRESIQQILLEEFGGESLEHQAQVSEGVLARLF
                     FVVKRPDDAGPALPVDVAKLQQRIEGVTRSWDDDFDRAAMHLASEARGVEFSDAYEEE
                     YPAATAVADLELANTLADEADRAYQVYAPIWPSDEVDVRFKVIGYRHMSLTDAMPHLS
                     VLGVEVVDERPY"
     gene            1864..2340
                     /locus_tag="PLF56_10470"
     CDS             1864..2340
                     /locus_tag="PLF56_10470"
                     /inference="COORDINATES: protein motif:HMM:NF022161.2"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="DUF2505 domain-containing protein"
                     /protein_id="HOQ54024.1"
                     /translation="MQIEAHNDFAADPVATHAMLTDPAFLSAVCIASGDLSHQVDAVA
                     DHSTVERQMPTPSAVKKFLGDTLTLVQNIHWHAPSANGARTGTLDLTVKGMPAKADVA
                     IDLRPGGRGTVVDFTGEFAIKVPFLGSKLEAQAAPALLEGFAVQQRVGDEWLAREH"
     gene            complement(2452..>3522)
                     /locus_tag="PLF56_10475"
     CDS             complement(2452..>3522)
                     /locus_tag="PLF56_10475"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_011753586.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_function: GO:0016491 - oxidoreductase activity
                     [Evidence IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="aldehyde dehydrogenase family protein"
                     /protein_id="HOQ54025.1"
                     /translation="ITPFNFTAIAGNLPTSAALMGNPVLWKPALTQMFAASVIVDVLR
                     AAGLPDGVINLVPGHGKEISDVALSHPDLAGIHFTGSTRVFQGLWGQVGAHIADYRSY
                     PRIVGETGGKDFVLAHPSADPAMLVTALIRGAFEYSGQKCSAASRAYLPRSLWTLVKD
                     DLIAQTEALSVGDVRDFANFTSAVIDERAFDKLSAAIDAARASSDAQIVAGGSYDRSE
                     GWYVRPSLIVSDNPKQDIFVTEYFGPILGIFVYEDAEFEGVLDLVDSASAYALTGSIL
                     ATDRAAVELAQQRLRFTAGNFYINDKPTGAVVGQQPFGGARASGTNDKAGSLWNLMRW
                     VSPRAIKETLVPPTSTGYPHML"
BASE COUNT          557 a         1241 c         1169 g          555 t
ORIGIN      
        1 catagggtcg ctcgtccacc acctcgacgc ccagcaccga caggtgcggc atggcgtccg
       61 tcagcgacat gtgccggtag ccgatcacct tgaaccgcac gtccacctcg tccgagggcc
      121 agatcggcgc gtacacctga taggcgcggt cggcctcgtc ggcgagggtg ttggcgagtt
      181 cgaggtcggc caccgccgtc gccgccggat actcctcctc gtaggcatcg ctgaactcga
      241 cgcctcgggc ttcgctggcc aggtgcatgg cggcgcggtc gaagtcgtcg tcccagctac
      301 gggtgacccc ctcgatccgc tgctgcagtt tcgccacgtc gaccggcagc gcgggcccgg
      361 cgtcgtccgg acgcttcacc acgaagaaca gccgcgccag cacaccctcg gacacctgag
      421 cctgatgctc gagcgactcg ccgccgaact cctcgagcag gatctgctga atcgactcgc
      481 gggtcgcggt ggtgtaccgg tcgcgtggca ggtacaccac caccgacgcg aatcggccgt
      541 aggcatcgcg gcgcacgaac accctgacca gccggcggtc cttgaactgg gcctcctgcg
      601 aaatcaccgg aaacagctcg tccacgctgg cctggaagag ctcgtcgcgg ggataggtgg
      661 cgatcacccg gcggatcgcg gagccgccat ggctgcgggg atcgaaaccc gagcgatcca
      721 tcagctgctg cgccttctgc gccaggatcg gaatcttgcg cacggtctcg ctgtacgcgg
      781 ccgccgagag cagtccgaga aatcgacgct cgccctgcag ttcgccgtgc gcgtcgtaca
      841 accgcacccc cacgtagtcg atgtaggccg gacgatgcac cggcgagcgc cgggaatcct
      901 tggtcagcac cagcaggtga tgatcgtcgg gccgcggaaa cgcgtcgaag ccgctcaccc
      961 cggggttgtc gtcgcgcaga atgcccagcc ccgtgcctgg cacgctgacg tagccgtcgc
     1021 cctcgcggcg gtactcgcga tagccgagca gcaccaggtt gccgcggccg agccactcga
     1081 gcaggtcggt gaccttctgg acgccgtgcg ggctcaccgg ttgcgggtgt tcgcccagcc
     1141 agtcgaccgt ctcggcgagc cggcgttgca tcggctgcca gtcgtcgacg gcggcctcga
     1201 cctgcgccag gcccgcggcc accatgtcgg cgagttgctc ggagctctcc tgcgaggcgt
     1261 ggccgagcgg cggaaagagt tcgacgcaga tccatgactc gggttcgcca cgcccgtccg
     1321 catcggccgc ggtgaggtgg ccgtccgcgt cgcgcaacac ctcgtactgc gggtggtaga
     1381 gcgctcggat gctccagttg ctgcgggtga tgttcatgct cacggtgtcg accaggaacg
     1441 gacgatcatc ggtcacgatc agcagcaacg tcgagacccg gtcgtccgac ggcggcacga
     1501 tgctgatctt cgcctcaccc ggacgccgca ccccaccgag cctgacgtgg cgccggatca
     1561 actcggccac gtgggccggg tcgagcgaga ccaactcacc ctcgggcaga tgctgcacgt
     1621 accgctcggc aaaccctggc agcaggtcgg cgtccgacga atccttcggt tggttgctca
     1681 agcaacggtt caactcgttg atccagtcgc tgcgtgacgc aattgctgct gccaccatgc
     1741 ctcctcgtcc gttgcacgcc gatgtgctca cgtcactcta tcgagcaatc gcgacctggg
     1801 cagatccccg gtcgcggggt ggggcggcgt ccgtccaccg gtgggtgggg cacaatggcc
     1861 ggcatgcaga tcgaggccca caacgacttc gccgccgacc ccgtcgccac gcatgcgatg
     1921 ctcaccgacc cggccttcct atcggctgtg tgtatcgcct cgggcgacct ctcgcaccag
     1981 gtggacgccg tcgccgatca cagcacggtc gaacgtcaga tgcccactcc gtccgcggtg
     2041 aagaagttcc tcggcgacac cctcaccctg gtgcagaaca tccactggca cgcgccgtcc
     2101 gccaacgggg cgcgcaccgg caccctcgac ctgaccgtca agggcatgcc ggccaaggcg
     2161 gacgtggcga tcgatcttcg tcccggcggc cgcggcacgg tggtcgattt caccggcgag
     2221 ttcgcgatca aggtgccgtt tctgggcagc aagctggagg ctcaggccgc cccggccctg
     2281 ctggagggct tcgccgtgca acagcgggtc ggcgacgaat ggctcgcgcg cgagcactga
     2341 ggtctgtgcc gggaccggat gcgcccgccg gagccgcccc gaagccctcc gtcagagcat
     2401 gtgcctccgt cagagccgcc ccgaagccct ccgtcagagc atgtgcctcc gtcagagcat
     2461 gtgtgggtag cccgtggacg tcgggggcac cagggtctcc ttgatcgctc gcgggctgac
     2521 ccagcgcatc aggttccaca ggctgcccgc cttgtcgttg gttccggacg ccctcgcccc
     2581 gccgaacggc tgctgcccca ccaccgcacc ggtcggcttg tcgttgatgt agaagttgcc
     2641 cgcggtgaac cgcagccgct gctgggccag ctcgacggcg gcccggtccg tggccaggat
     2701 cgaacccgtc agggcgtacg ccgacgccga gtccaccagg tcgagcaccc cttcgaactc
     2761 ggcgtcctcg tacacgaaga tgcccaggat cggaccgaag tactcggtga cgaagatgtc
     2821 ctgcttgggg ttgtccgaga cgatcagcga ggggcgcacg taccaccctt cgctgcggtc
     2881 gtagctgccg cccgccacga tctgcgcatc ggacgatgcc cgcgcggcgt cgatcgccgc
     2941 cgacagcttg tcgaaggccc gctcgtcgat caccgccgag gtgaagttgg cgaagtcgcg
     3001 cacgtcgccc accgacagcg cctcggtctg ggcgatcaga tcgtccttca ccagggtcca
     3061 cagcgagcgc ggcaggtacg ccctggacgc cgccgagcac ttctgtcccg agtactcgaa
     3121 ggccccccgg atcagcgcgg tgaccagcat cgccgggtcg gcgctgggat gggcgagcac
     3181 gaagtccttg ccgccggtct cgcccacgat gcgcgggtag ctgcggtagt cggcgatgtg
     3241 ggcgccgacc tgaccccaca gcccctggaa gaccctggtc gagccggtga agtgaatgcc
     3301 ggccaggtcg gggtgcgaga gtgcgacgtc cgaaatctcc ttgccgtgcc ccggtaccag
     3361 gttgatcacc ccgtcgggca ggccggcggc gcgcagcacg tccacgatca cggacgcggc
     3421 gaacatctgg gtcaacgcgg gcttccagag caccgggttg cccatcagcg cggccgaggt
     3481 gggcaggttg cccgcgatcg cggtgaagtt gaacggggtg at
//
LOCUS       DAOSUG010000331         3509 bp    DNA     linear   ENV 10-JUN-2024
DEFINITION  MAG TPA_asm: Micropruina sp. isolate AMR_MDS_2195
            NODE_10946_length_3509_cov_4.843370, whole genome shotgun sequence.
ACCESSION   DAOSUG010000331 DAOSUG010000000
VERSION     DAOSUG010000331.1
DBLINK      BioProject: PRJNA925477
            BioSample: SAMN32864262
            Sequence Read Archive: SRR4236673
KEYWORDS    WGS; ENV; Metagenome Assembled Genome; MAG; Third Party Data; TPA;
            TPA:assembly.
SOURCE      Micropruina sp. (wastewater metagenome)
  ORGANISM  Micropruina sp.
            Bacteria; Bacillati; Actinomycetota; Actinomycetes;
            Propionibacteriales; Nocardioidaceae; Micropruina.
REFERENCE   1  (bases 1 to 3509)
  AUTHORS   Abdulkadir,N., Saraiva,J.P., Zhang,J., Stolte,S., Gillor,O.,
            Harms,H. and Rocha,U.
  TITLE     Genome-centric analyses of 165 metagenomes show that mobile genetic
            elements are crucial for the transmission of antimicrobial
            resistance genes to pathogens in activated sludge and wastewater
  JOURNAL   Microbiol Spectr 12 (3), e0291823 (2024)
   PUBMED   38289113
REFERENCE   2  (bases 1 to 3509)
  AUTHORS   Abdulkadir,N.
  TITLE     Direct Submission
  JOURNAL   Submitted (24-MAR-2023) Environmental Microbiology, Helmholtz
            Center for Environmental Research -UFZ, Permoserstr 15, Leipzig
            04318, Germany
COMMENT     The annotation was added by the NCBI Prokaryotic Genome Annotation
            Pipeline (PGAP). Information about PGAP can be found here:
            https://www.ncbi.nlm.nih.gov/genome/annotation_prok/
            
            ##Genome-Assembly-Data-START##
            Assembly Method        :: SPAdes v. 3.11.1
            Genome Representation  :: Full
            Expected Final Version :: Yes
            Genome Coverage        :: Not Applicable
            Sequencing Technology  :: Illumina MiSeq
            ##Genome-Assembly-Data-END##
            
            ##Genome-Annotation-Data-START##
            Annotation Provider               :: NCBI
            Annotation Date                   :: 05/04/2023 04:57:10
            Annotation Pipeline               :: NCBI Prokaryotic Genome
                                                 Annotation Pipeline (PGAP)
            Annotation Method                 :: Best-placed reference protein
                                                 set; GeneMarkS-2+
            Annotation Software revision      :: 6.5
            Features Annotated                :: Gene; CDS; rRNA; tRNA; ncRNA
            Genes (total)                     :: 2,776
            CDSs (total)                      :: 2,750
            Genes (coding)                    :: 2,714
            CDSs (with protein)               :: 2,714
            Genes (RNA)                       :: 26
            tRNAs                             :: 24
            ncRNAs                            :: 2
            Pseudo Genes (total)              :: 36
            CDSs (without protein)            :: 36
            Pseudo Genes (ambiguous residues) :: 0 of 36
            Pseudo Genes (frameshifted)       :: 2 of 36
            Pseudo Genes (incomplete)         :: 32 of 36
            Pseudo Genes (internal stop)      :: 2 of 36
            ##Genome-Annotation-Data-END##
FEATURES             Location/Qualifiers
     source          1..3509
                     /organism="Micropruina sp."
                     /mol_type="genomic DNA"
                     /submitter_seqid="NODE_10946_length_3509_cov_4.843370"
                     /isolate="AMR_MDS_2195"
                     /isolation_source="Activated sludge of municipal
                     wastewater treatment plants"
                     /db_xref="taxon:2737536"
                     /environmental_sample
                     /geo_loc_name="Canada"
                     /lat_lon="49.25 N 123.1 W"
                     /collection_date="2015-06-16"
                     /metagenome_source="wastewater metagenome"
                     /note="metagenomic"
     gene            <1..314
                     /locus_tag="PLF56_10480"
     CDS             <1..314
                     /locus_tag="PLF56_10480"
                     /inference="COORDINATES: ab initio
                     prediction:GeneMarkS-2+"
                     /note="Derived by automated computational analysis using
                     gene prediction method: GeneMarkS-2+."
                     /codon_start=3
                     /transl_table=11
                     /product="alpha/beta hydrolase"
                     /protein_id="HOQ54026.1"
                     /translation="TPEPPALADAPLARPSVADHLLPTVLRGAASSNLPPLQTIATIE
                     VPTLILAWADDPAHPLRTAELLHQALPHSRRVVARTPYGIMAWPALFADHVTSIEARV
                     G"
     gene            complement(643..>3509)
                     /locus_tag="PLF56_10485"
     CDS             complement(643..>3509)
                     /locus_tag="PLF56_10485"
                     /EC_number="3.6.4.-"
                     /inference="COORDINATES: protein motif:HMM:NF012403.2"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_function: GO:0004386 - helicase activity [Evidence
                     IEA]"
                     /codon_start=3
                     /transl_table=11
                     /product="DEAD/DEAH box helicase"
                     /protein_id="HOQ54027.1"
                     /translation="AWQLALTEVTADAKPAGQGQPLAIAVARPAGMVQLKAMVWGRSG
                     RWVKSGITWDQVAYPWQGDYREAHRQALAGLARTRDQRSSTSYYTRRADELWLSELGP
                     SAWYWLREALDAGVVLVPGQGVNRVRLSEQRARLVSRVVRTQGGLGLRTLAVTDEVER
                     AVPYADLLGDPPHGFVVESPEELLLVPFAEPLSKGQRTLLVQHARLEIPEAEVPTFAA
                     QFYPTLRRIGHVELDDDVDLPQPEPPALVLRVQFLADHVTHLRWGFRYRVGGAALDAP
                     LATGMDPAGYRDVDAEARLLATLPEGPWPVARVGETARPVESTTLTGPATAQLAELWL
                     DRLRAQGVIVEVSGEPVEYRLATEAPEVTLRVSDPPEGTDWFNLAVQVTIEGEPVEYR
                     ELFTALATGQTHLILPSGTWFSLERPELEQLRALITEAQELNGGDDDGQLRLRTEHAG
                     LWDELVQLGVVAEQSAAWTRAASALLDLTELPQPAVPDGLAATLRPYQEVGFRWLHFL
                     YTSRLGGILADDMGLGKTVQALALACAIKEAGELDRPFLVVAPTSVLGTWAEQAARFA
                     PGLRVRVVTQTAKKRDQPLAEVAGDADIVVTSYTLVRLDEDEYVEQPWSVVLLDEAQF
                     VKNRQARGHQSVRKLRARTKFAITGTPLENNLMDLWALLSIVSPGLFADPVLFGERYR
                     RPIENGSDHDALARLHRRVRPLMLRRTKETVAAELPPKQEQVLSIELSPAHRRLYDKQ
                     LAAERKKVLGLVGDLQRNRITILAALTTLRQLALSPALVLPGQAAVSAKIDTLIELVT
                     ELAAEGHRALVFSQFTTYLGMVRERLAAEKIGATYLDGRTRDRAARIEQFRTGDDPVF
                     LISLKAGGFGLTLTEADYVFILDPWWNPAAELQAIDRTHRIGQDKHVMVYRMVSADTI
                     EEKVVALQQRKRDLFDQVVGKGGDLAAPLTADDIRGLLEP"
BASE COUNT          520 a         1298 c         1165 g          526 t
ORIGIN      
        1 aaacccctga accgcccgcg ctggccgacg ccccgctggc ccgtccgagc gttgccgacc
       61 acctgctgcc caccgtgctg cggggtgcgg cctcgtccaa cctgccgccg ttgcagacga
      121 tcgccacgat cgaggtgccc acgctgatcc tggcctgggc cgacgatccc gcacacccgt
      181 tgcgcaccgc ggaactgctg caccaggcac tgccccacag ccgccgggtg gtggcccgca
      241 ccccctacgg catcatggcg tggccggcat tgttcgccga tcacgtcacc tcgatcgagg
      301 cgcgggtcgg ctaggtctgg cgcacacccg agcaaccggg ctgccggtga cgacatgccg
      361 atcacggcac gatccgtcgg tcgagcctgt cgaaccgctg aggcgtgtct cgagctcccg
      421 ggatgactgc caatacccga tgatgtcgcg gtacaacacg aaatcgaagc ggcaagccga
      481 aaccctgtgg aggtgacggg agctctcgga ctgccggtgc gacgccggcg atctcgagat
      541 cgctgcacga tttcgcccaa acccgacgat gtcgccctac aacacgaaat cgaagcggca
      601 agccgaaacc ctgtggcgat ctcgagatcg tccatgcccc ccttacggct ccagcaggcc
      661 ccggatgtcg tccgcggtca acggggcggc caggtcgccg cccttgccga ccacctggtc
      721 gaacaggtcg cgcttgcgtt gctgcagcgc gaccaccttc tcttcgatgg tgtcggcgct
      781 gaccattcgg tagaccatca cgtgcttgtc ctggccgatg cgatgggtgc ggtcgatcgc
      841 ctgcaactcg gcggccgggt tccaccacgg gtcgagaatg aacacgtagt cggcctcggt
      901 cagcgtcagc ccgaagccgc ccgccttgag gctgatcaga aacaccgggt cgtcgccggt
      961 gcggaactgc tcgatccgcg ccgcccggtc gcgggtgcgt ccgtccagat aggtggcccc
     1021 gatcttctcg gccgccagcc gctcgcgcac catgcccagg tacgtggtga actggctgaa
     1081 caccaacgcc cggtgcccct cggcggccaa ctcggtgacc agctcgatca gcgtgtcgat
     1141 cttggctgag accgcagcct ggcccggcag caccaaagcc ggcgacagtg ccagctgccg
     1201 cagggtggtg agcgccgcca gaatcgtgat ccggttgcgc tgcaggtcac cgaccagccc
     1261 gagcaccttc ttgcgctcgg ccgcgagttg cttgtcgtag agccgccggt gcgccggcga
     1321 gagttcgatc gacagcacct gctcctgctt gggcggcagt tcggcggcca ccgtctcttt
     1381 cgtgcgccgc agcatcagcg gacgcacccg ccggtgcagc cgggcgagcg cgtcgtggtc
     1441 cgagccgttc tcgatcggac gccggtaccg ctccccgaac agcaccgggt cggcgaacag
     1501 gcccggcgac acgatcgaca gcagcgccca caggtccatc aggttgttct ccagcggcgt
     1561 gccggtgatc gcgaacttgg tgcgggcccg cagcttgcgc accgactggt gcccccgggc
     1621 ctgccggttc ttgacgaact gcgcctcgtc cagcagcacc accgaccacg gctgctcgac
     1681 gtactcgtcc tcgtcgagcc gcaccagcgt gtacgaggtg accacgatgt cggcgtcccc
     1741 ggccacctcg gccagcggct ggtcccgctt cttggccgtc tgcgtcacca cccggacgcg
     1801 cagccccggc gcgaaccgcg ccgcctgctc ggcccacgtg cccagcacgg acgtgggcgc
     1861 caccaccagg aacggacgat ccaactcgcc ggcctccttg atcgcgcagg ccagcgccag
     1921 cgcctgcacg gtcttgccca ggcccatgtc gtcggcgagg atgccgccca ggcgactggt
     1981 gtacaagaag tgcagccagc gaaacccgac ctcctgatac ggacgaaggg tggccgccag
     2041 tccgtccggc accgccggct gcggcagttc ggtcaggtcg agcagcgcgg acgccgcccg
     2101 cgtccaggcc gccgactgct ccgcgaccac gcccagctgg acgagctcgt cccacagccc
     2161 ggcatgctcg gtgcgcagcc gcagctggcc gtcatcgtcc cccccgttca gctcctgcgc
     2221 ctcggtgatc agcgcccgca actgctcgag ttcagggcgt tccaggctga accacgtgcc
     2281 ggacggcagg atcagatgcg tctgcccggt ggccagcgcg gtgaacaact cgcggtactc
     2341 gaccggctcg ccctcgatcg tgacctggac cgcgaggttg aaccagtcgg tgccctcggg
     2401 cggatcgctc acccgaaggg tgacttcggg cgcctctgtg gccagccggt actcgaccgg
     2461 ttcgccgctc acctcgacga tcactccttg cgcgcgcagg cggtccagcc acagttccgc
     2521 cagttgtgcc gtcgccgggc cggtcagggt tgtgctctcg actggacggg ccgtctcccc
     2581 cacccgcgcc accggccacg gtccctcggg cagggtggcc agcaggcgcg cctcggcgtc
     2641 gacgtcgcgg tagccggccg ggtccatgcc ggtggccagg ggtgcgtcca gggcggcgcc
     2701 cccgacccgg tagcgaaagc cccagcgcag gtgcgtcacg tggtcagcca agaactgcac
     2761 ccgtagcacc agggccgggg gttcgggttg gggcaggtcg acgtcatcgt ccagttcgac
     2821 gtgcccgatc cggcgcagcg tggggtagaa ctgggcggcg aacgtgggca cctcggcctc
     2881 gggaatctcc agccgggcat gctgcaccag cagcgtgcgt tggcccttgc tcagcggctc
     2941 ggcgaacggt accagcagca actcctcggg cgactcgacg acgaagccgt gcggcgggtc
     3001 gccgagcagg tcggcatagg gcacggcgcg ctccacctcg tcggtgaccg ccagcgtgcg
     3061 cagccccagg ccaccctggg tacggacgac ccggctgacc agccgagcgc gctgctccga
     3121 caggcgcacc cggttcactc cttggccggg cacgagcacc accccggcat cgagtgcttc
     3181 gcgcaaccag taccaggccg acggcccgag ttcgctcagc cacagttcgt ccgcacgcct
     3241 ggtgtagtag ctggtgctgc tgcgctggtc gcgggttcgg gccagcccgg ccagcgcctg
     3301 ccggtgcgcc tcgcggtagt cgccctgcca ggggtaggcg acctggtccc aggtgatgcc
     3361 cgacttgacc cagcgaccgg agcgtcccca gaccatggcc ttgagctgca ccatgccggc
     3421 gggccgcgcc acggcgattg cgagcggctg gccttggccg gccggcttgg cgtcggcggt
     3481 cacctcggtg agggccagtt gccaggccg
//
LOCUS       DAOSUG010000332         3495 bp    DNA     linear   ENV 10-JUN-2024
DEFINITION  MAG TPA_asm: Micropruina sp. isolate AMR_MDS_2195
            NODE_11008_length_3495_cov_4.583140, whole genome shotgun sequence.
ACCESSION   DAOSUG010000332 DAOSUG010000000
VERSION     DAOSUG010000332.1
DBLINK      BioProject: PRJNA925477
            BioSample: SAMN32864262
            Sequence Read Archive: SRR4236673
KEYWORDS    WGS; ENV; Metagenome Assembled Genome; MAG; Third Party Data; TPA;
            TPA:assembly.
SOURCE      Micropruina sp. (wastewater metagenome)
  ORGANISM  Micropruina sp.
            Bacteria; Bacillati; Actinomycetota; Actinomycetes;
            Propionibacteriales; Nocardioidaceae; Micropruina.
REFERENCE   1  (bases 1 to 3495)
  AUTHORS   Abdulkadir,N., Saraiva,J.P., Zhang,J., Stolte,S., Gillor,O.,
            Harms,H. and Rocha,U.
  TITLE     Genome-centric analyses of 165 metagenomes show that mobile genetic
            elements are crucial for the transmission of antimicrobial
            resistance genes to pathogens in activated sludge and wastewater
  JOURNAL   Microbiol Spectr 12 (3), e0291823 (2024)
   PUBMED   38289113
REFERENCE   2  (bases 1 to 3495)
  AUTHORS   Abdulkadir,N.
  TITLE     Direct Submission
  JOURNAL   Submitted (24-MAR-2023) Environmental Microbiology, Helmholtz
            Center for Environmental Research -UFZ, Permoserstr 15, Leipzig
            04318, Germany
COMMENT     The annotation was added by the NCBI Prokaryotic Genome Annotation
            Pipeline (PGAP). Information about PGAP can be found here:
            https://www.ncbi.nlm.nih.gov/genome/annotation_prok/
            
            ##Genome-Assembly-Data-START##
            Assembly Method        :: SPAdes v. 3.11.1
            Genome Representation  :: Full
            Expected Final Version :: Yes
            Genome Coverage        :: Not Applicable
            Sequencing Technology  :: Illumina MiSeq
            ##Genome-Assembly-Data-END##
            
            ##Genome-Annotation-Data-START##
            Annotation Provider               :: NCBI
            Annotation Date                   :: 05/04/2023 04:57:10
            Annotation Pipeline               :: NCBI Prokaryotic Genome
                                                 Annotation Pipeline (PGAP)
            Annotation Method                 :: Best-placed reference protein
                                                 set; GeneMarkS-2+
            Annotation Software revision      :: 6.5
            Features Annotated                :: Gene; CDS; rRNA; tRNA; ncRNA
            Genes (total)                     :: 2,776
            CDSs (total)                      :: 2,750
            Genes (coding)                    :: 2,714
            CDSs (with protein)               :: 2,714
            Genes (RNA)                       :: 26
            tRNAs                             :: 24
            ncRNAs                            :: 2
            Pseudo Genes (total)              :: 36
            CDSs (without protein)            :: 36
            Pseudo Genes (ambiguous residues) :: 0 of 36
            Pseudo Genes (frameshifted)       :: 2 of 36
            Pseudo Genes (incomplete)         :: 32 of 36
            Pseudo Genes (internal stop)      :: 2 of 36
            ##Genome-Annotation-Data-END##
FEATURES             Location/Qualifiers
     source          1..3495
                     /organism="Micropruina sp."
                     /mol_type="genomic DNA"
                     /submitter_seqid="NODE_11008_length_3495_cov_4.583140"
                     /isolate="AMR_MDS_2195"
                     /isolation_source="Activated sludge of municipal
                     wastewater treatment plants"
                     /db_xref="taxon:2737536"
                     /environmental_sample
                     /geo_loc_name="Canada"
                     /lat_lon="49.25 N 123.1 W"
                     /collection_date="2015-06-16"
                     /metagenome_source="wastewater metagenome"
                     /note="metagenomic"
     gene            <1..361
                     /locus_tag="PLF56_10490"
     CDS             <1..361
                     /locus_tag="PLF56_10490"
                     /inference="COORDINATES: ab initio
                     prediction:GeneMarkS-2+"
                     /note="Derived by automated computational analysis using
                     gene prediction method: GeneMarkS-2+."
                     /codon_start=2
                     /transl_table=11
                     /product="hypothetical protein"
                     /protein_id="HOQ54028.1"
                     /translation="KALLATQAFQVQGQVTGKDVVAAYLKQITESMTGATTTKQPATL
                     DVKADRLSVGIAGWSGTRATAQGSVKLQYVSIISVRDDGLAVMSTLIVPSGSTVSAQQ
                     ADYLAMTNSLLSSQLQA"
     gene            531..2609
                     /gene="uvrB"
                     /locus_tag="PLF56_10495"
     CDS             531..2609
                     /gene="uvrB"
                     /locus_tag="PLF56_10495"
                     /EC_number="3.1.25.-"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_015582967.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_component: GO:0009380 - excinuclease repair complex
                     [Evidence IEA];
                     GO_function: GO:0003677 - DNA binding [Evidence IEA];
                     GO_function: GO:0005515 - protein binding [Evidence IEA];
                     GO_function: GO:0005524 - ATP binding [Evidence IEA];
                     GO_function: GO:0016787 - hydrolase activity [Evidence
                     IEA];
                     GO_function: GO:0016887 - ATP hydrolysis activity
                     [Evidence IEA];
                     GO_process: GO:0006289 - nucleotide-excision repair
                     [Evidence IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="excinuclease ABC subunit UvrB"
                     /protein_id="HOQ54029.1"
                     /translation="MRPTTELQRRVAPFKVQADFAPAGDQPAAIDELERRVKAGESDV
                     VVLGATGTGKTATVAWLAERLQRPMLVMQPNKTLAPQFANELRTFFPDNAVEYFVSYY
                     DYYQPEAYVPQTDTYIEKDSSLNEEVERLRHSATSSLLTRRDVIVVATVSAIYGLGTP
                     QEYVDRMERLRVGDVRDRDQLLRSLVDIQYARNDLAGTRGTFRVRGDTVEIFPMYEEN
                     AVRIEFFGDEIEALSTLHPLTGQVLSVDQEVAIFPASHYVAGPERMERAITGIELELG
                     GRLAQLEGEQKLLEAQRLRMRTGYDIEMMRQIGTCSGIENYSRHIDGRSSGSAPNCLL
                     DYFPSDFVLVVDESHVSIPQIGGMYEGDASRKRTLVEHGFRLPSAMDNRPLTFEEFTD
                     RIGQTVYLSATPGPYEMARSGSPVELLIRPTGLVDPEVILKPTKGQIDDLIGEIRLRA
                     ERNERVLVTTLTKKMSEDLTDYLMENGIRTRYLHSEVDTLKRIELLRELRLGEYDVLV
                     GINLLREGLDLPEVSLVAILDADKEGFLRSERSLIQTIGRAARNVNGQVHMYADKITP
                     SMAAAIDETNRRRAKQVAYNLERGVDPQPLRKKIADITDMLAREDADTAALVTRAGGR
                     RVSPVPLGNHQVRNTDGMPASELAGLVQELTEQMHGAAAELQFELAARLRDEIADLKK
                     MIRQTLAATK"
     gene            2780..>3495
                     /locus_tag="PLF56_10500"
     CDS             2780..>3495
                     /locus_tag="PLF56_10500"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_012920734.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_component: GO:0016020 - membrane [Evidence IEA];
                     GO_function: GO:0003674 - molecular_function [Evidence
                     IEA];
                     GO_process: GO:0008150 - biological_process [Evidence
                     IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="TerC/Alx family metal homeostasis membrane
                     protein"
                     /protein_id="HOQ54030.1"
                     /translation="MHVTPTVWAITILATVALLAFDLIVIGRRPHEPSMKECSIAISI
                     YVGMAVAFGIGVWSTSGGRFAGEFFAGWLTEYSLSIDNLFIFVIIMSNLRVPRKYQQY
                     ALMVGIVLALLFRGIFIWLGAAAIERFSWIFFVFGAFLIWTAWKLFMDYLHPEDDDEK
                     TENFAMRWVKKTFPSTEQYHGTKLTIVENGKRLITPMLFVIIALGSTDLLFALDSIPA
                     IYGLTQEPFLVFTANVFALMG"
BASE COUNT          642 a         1150 c         1114 g          589 t
ORIGIN      
        1 aaaggcactg ctggccaccc aggcgttcca ggtgcagggg caggtgaccg gcaaggacgt
       61 ggtggccgcc tacctgaagc agatcaccga gagcatgacc ggcgcgacca ccaccaagca
      121 gcccgccaca ttggacgtga aggccgaccg gctctcggtg ggcatcgcag gctggtcggg
      181 cacccgggcg accgcgcagg gttcggtcaa gctgcagtac gtcagcatca tctcggtgcg
      241 cgacgacggc ctggcggtga tgtccaccct gatcgtgccc tccggcagca ccgtgtcggc
      301 gcagcaggcg gactatctgg cgatgacgaa ctcactgctg agcagccagt tgcaggcctg
      361 atccaccggg agttcccgaa ccctcgcctc accatcgtgc gcctgcgtag cttcgcaggc
      421 gcacgatggt gaggcgcggc gttgcgtcgg gaccggacgc cgcggtcggc gggtgctccg
      481 accggggacg ggccagaaaa ctgtcggcgc cggcgcctag gcttgccgac atgcgtccca
      541 ccaccgaact gcagcgccgc gtggccccct tcaaggtgca ggccgacttc gcgccggccg
      601 gcgaccagcc ggccgccatc gacgagctcg aacgccgcgt caaggcgggc gagagcgacg
      661 tcgtggtgct gggcgccacc ggtaccggca aaaccgcaac cgtcgcatgg ctggccgagc
      721 ggctgcagcg tccgatgctg gtgatgcaac ccaacaagac cctggcgccc cagttcgcga
      781 acgagttgcg cacgttcttt cccgacaacg cggtcgagta cttcgtgtcg tactacgact
      841 attaccaacc cgaggcgtac gttccccaga ccgacaccta catcgagaag gattcgtcgc
      901 tcaacgaaga ggtggagcgg ctacggcact cggccaccag ttcgctgctc acccggcgcg
      961 acgtcatcgt ggtggcgacg gtgtcggcca tctacggcct cggcacacct caggagtacg
     1021 tcgatcgtat ggagcggctg cgggtcggcg acgtgcgcga tcgcgaccag ttgcttcgct
     1081 ccctggtcga catccagtac gcgcgcaacg acctggcggg cacccggggc acgttccggg
     1141 tgcgtggcga tacggtcgag atctttccga tgtacgaaga gaacgccgtg cgcatcgagt
     1201 tcttcggcga cgagatcgag gcactctcga cgttgcatcc gttgaccggc caggtgctct
     1261 cggtcgacca ggaggtggct atctttccgg cctcccacta tgtcgcgggc cccgagcgta
     1321 tggagcgtgc aatcaccggc atcgaactcg aactgggggg ccggttggcc cagctcgagg
     1381 gtgagcagaa gctgctcgaa gcgcaacgac tgcgcatgcg caccgggtat gacatcgaga
     1441 tgatgcgtca gatcggcacc tgttccggca tcgagaacta ctcccggcac attgatggac
     1501 gatcctcggg cagcgcgccc aactgcctgc tcgactactt tccgtccgac ttcgtgctgg
     1561 tggtcgacga gtcgcacgtc agcattccgc agatcggcgg catgtacgag ggcgacgcct
     1621 cgcgcaagcg caccctggtc gagcacggct tccggctgcc cagcgccatg gacaaccgcc
     1681 cgctgacctt cgaagagttc accgaccgca tcggccagac cgtctacctc tcggccacgc
     1741 cggggcccta cgagatggca cgctccgggt cgcccgtcga gttgttgatc cggcccaccg
     1801 gtttggtcga ccccgaggtc attctcaaac cgaccaaggg tcagatcgac gacctgatcg
     1861 gtgagatccg gctgcgtgcc gaacgcaacg aacgcgttct ggtcaccacg ctgaccaaga
     1921 agatgtccga agacctcacc gactatctga tggagaacgg catccgcacc cggtacctgc
     1981 actccgaggt cgacactctc aaacgcatcg agttgttgcg cgagctgcgg ctgggcgaat
     2041 acgacgtact ggtcggcatc aacctgctgc gcgagggcct cgacctgccc gaggtcagcc
     2101 tggtggcgat tctggacgcc gacaaggagg gctttctgcg tagcgagcgg tcgctgatcc
     2161 agaccatcgg acgtgccgcc cgcaacgtca acggccaggt gcacatgtac gccgacaaga
     2221 tcacgccctc catggcggca gcgatcgacg aaaccaaccg acggcgggcc aagcaggtgg
     2281 cctacaacct cgaacggggg gtagacccgc aaccgctgcg caagaagatc gccgacatca
     2341 ccgacatgct ggcccgtgaa gacgccgaca cggcggcgct ggtcaccagg gccggtggcc
     2401 gccgggtctc accggtgccg ttgggcaacc accaggtgcg caacaccgac ggcatgccgg
     2461 ccagcgaact ggccggcctg gtgcaagaac tcaccgagca gatgcacggt gcggcggccg
     2521 aactgcagtt cgagctcgcc gcccgactgc gcgatgagat cgccgacctg aagaagatga
     2581 tccgccagac gctggcggcc accaaatgat cacccttgcg ggcgtttggc cgcaggcccg
     2641 ggcaatgcga tgatttatct cgacgagggg agtactccca tagcgaggcg tcgtcagcac
     2701 gagcggtgat ccgctccggt gtctcggctg gcgacagcgg cgaagacctc agacaactgg
     2761 accttgagga ggcccttcct tgcacgtcac acccaccgtt tgggcgatca ccatcctggc
     2821 tactgtcgcg ctgttggcct tcgacctgat cgtgatcgga cggcgccccc acgagccgtc
     2881 catgaaggaa tgctcgatcg ccatctcgat ctacgtcggc atggcggtgg cgttcggaat
     2941 cggtgtctgg agcacgtccg ggggccgatt cgcgggcgag ttcttcgcgg ggtggctcac
     3001 cgagtacagc ctcagcatcg acaacctgtt catcttcgtg atcatcatga gcaacctgcg
     3061 ggtaccgcgt aagtaccagc agtacgcact gatggtgggc atcgtgctgg cgctgctgtt
     3121 ccggggcatc ttcatctggc tgggtgcggc cgccatcgag cggttctcgt ggatcttctt
     3181 cgtattcgga gccttcctga tctggacggc ctggaagctg ttcatggact acctgcatcc
     3241 cgaggacgac gacgagaaga ccgagaactt cgccatgcgc tgggtgaaga agacctttcc
     3301 gtccaccgag cagtaccacg gcacgaagct caccatcgtt gagaacggca agcggctgat
     3361 cacaccgatg ctgttcgtga tcatcgctct cggcagcacc gatctactgt tcgcgctcga
     3421 ctcgattccg gcgatctacg gcctgaccca ggagccgttc ctggtgttca ccgccaacgt
     3481 gttcgcgctg atggg
//
LOCUS       DAOSUG010000333         3495 bp    DNA     linear   ENV 10-JUN-2024
DEFINITION  MAG TPA_asm: Micropruina sp. isolate AMR_MDS_2195
            NODE_11009_length_3495_cov_4.193895, whole genome shotgun sequence.
ACCESSION   DAOSUG010000333 DAOSUG010000000
VERSION     DAOSUG010000333.1
DBLINK      BioProject: PRJNA925477
            BioSample: SAMN32864262
            Sequence Read Archive: SRR4236673
KEYWORDS    WGS; ENV; Metagenome Assembled Genome; MAG; Third Party Data; TPA;
            TPA:assembly.
SOURCE      Micropruina sp. (wastewater metagenome)
  ORGANISM  Micropruina sp.
            Bacteria; Bacillati; Actinomycetota; Actinomycetes;
            Propionibacteriales; Nocardioidaceae; Micropruina.
REFERENCE   1  (bases 1 to 3495)
  AUTHORS   Abdulkadir,N., Saraiva,J.P., Zhang,J., Stolte,S., Gillor,O.,
            Harms,H. and Rocha,U.
  TITLE     Genome-centric analyses of 165 metagenomes show that mobile genetic
            elements are crucial for the transmission of antimicrobial
            resistance genes to pathogens in activated sludge and wastewater
  JOURNAL   Microbiol Spectr 12 (3), e0291823 (2024)
   PUBMED   38289113
REFERENCE   2  (bases 1 to 3495)
  AUTHORS   Abdulkadir,N.
  TITLE     Direct Submission
  JOURNAL   Submitted (24-MAR-2023) Environmental Microbiology, Helmholtz
            Center for Environmental Research -UFZ, Permoserstr 15, Leipzig
            04318, Germany
COMMENT     The annotation was added by the NCBI Prokaryotic Genome Annotation
            Pipeline (PGAP). Information about PGAP can be found here:
            https://www.ncbi.nlm.nih.gov/genome/annotation_prok/
            
            ##Genome-Assembly-Data-START##
            Assembly Method        :: SPAdes v. 3.11.1
            Genome Representation  :: Full
            Expected Final Version :: Yes
            Genome Coverage        :: Not Applicable
            Sequencing Technology  :: Illumina MiSeq
            ##Genome-Assembly-Data-END##
            
            ##Genome-Annotation-Data-START##
            Annotation Provider               :: NCBI
            Annotation Date                   :: 05/04/2023 04:57:10
            Annotation Pipeline               :: NCBI Prokaryotic Genome
                                                 Annotation Pipeline (PGAP)
            Annotation Method                 :: Best-placed reference protein
                                                 set; GeneMarkS-2+
            Annotation Software revision      :: 6.5
            Features Annotated                :: Gene; CDS; rRNA; tRNA; ncRNA
            Genes (total)                     :: 2,776
            CDSs (total)                      :: 2,750
            Genes (coding)                    :: 2,714
            CDSs (with protein)               :: 2,714
            Genes (RNA)                       :: 26
            tRNAs                             :: 24
            ncRNAs                            :: 2
            Pseudo Genes (total)              :: 36
            CDSs (without protein)            :: 36
            Pseudo Genes (ambiguous residues) :: 0 of 36
            Pseudo Genes (frameshifted)       :: 2 of 36
            Pseudo Genes (incomplete)         :: 32 of 36
            Pseudo Genes (internal stop)      :: 2 of 36
            ##Genome-Annotation-Data-END##
FEATURES             Location/Qualifiers
     source          1..3495
                     /organism="Micropruina sp."
                     /mol_type="genomic DNA"
                     /submitter_seqid="NODE_11009_length_3495_cov_4.193895"
                     /isolate="AMR_MDS_2195"
                     /isolation_source="Activated sludge of municipal
                     wastewater treatment plants"
                     /db_xref="taxon:2737536"
                     /environmental_sample
                     /geo_loc_name="Canada"
                     /lat_lon="49.25 N 123.1 W"
                     /collection_date="2015-06-16"
                     /metagenome_source="wastewater metagenome"
                     /note="metagenomic"
     gene            315..812
                     /locus_tag="PLF56_10505"
     CDS             315..812
                     /locus_tag="PLF56_10505"
                     /inference="COORDINATES: ab initio
                     prediction:GeneMarkS-2+"
                     /note="Derived by automated computational analysis using
                     gene prediction method: GeneMarkS-2+."
                     /codon_start=1
                     /transl_table=11
                     /product="hypothetical protein"
                     /protein_id="HOQ54031.1"
                     /translation="MAGTLRRFCVTLCVPLALALTGCIAQFQTSQPYTPGEGVNLQVG
                     QVKVRNLMVIADASGQGVLSASAVSYADDTLTAVSGTPHKADGTPGSALVVTSTGLPL
                     AMPANTLFILTDSPLRVAVTSPDLKPGLLAEITLTFAKAGDVKALAPVVSSSEPEYSG
                     ITVGS"
     gene            complement(1375..2052)
                     /locus_tag="PLF56_10510"
     CDS             complement(1375..2052)
                     /locus_tag="PLF56_10510"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_004810606.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_function: GO:0003677 - DNA binding [Evidence IEA];
                     GO_process: GO:0000160 - phosphorelay signal transduction
                     system [Evidence IEA];
                     GO_process: GO:0006355 - regulation of DNA-templated
                     transcription [Evidence IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="response regulator transcription factor"
                     /protein_id="HOQ54032.1"
                     /translation="MTRVLLIEDEDAYRETVGYLLRKEGFDVVEAADGTIGLAEFERG
                     GADIVLLDLMMPGLPGTEVCRRLRARSSVPVIMVTARDDEIDKVVGLELGADDYVTKP
                     FSHRELVARIRAVLRRGQDFELEPDVLEVDAVRMDVERHEVWVRGEPVKLALKEFELL
                     EILLRNAGRVMTRGQLIDRIWGADYVGDTKTLDVHVKRLRAKVETDPAEPQLLVTVRG
                     LGYKFEA"
     gene            complement(2049..3203)
                     /locus_tag="PLF56_10515"
     CDS             complement(2049..3203)
                     /locus_tag="PLF56_10515"
                     /inference="COORDINATES: protein motif:HMM:NF014567.2"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="ATP-binding protein"
                     /protein_id="HOQ54033.1"
                     /translation="MNPLVACALGVVLGVLVTLFAMTLRRARQQASVAQEPRPLLAPQ
                     VRLVVEHLRGAAAVLGPHDELLAQSHSAASSGVVRGSRVAQPEVLELVREARRDEEPE
                     ALNIDVPTGLGLPARRLAVRVAPLGDGLVLLVADDRSEALRVDETRRDFVANITHELK
                     TPIGAISLLAEAVAASADDADAVRHFAGRMATETARLDELISQIIALSRLQSTDPLLA
                     AVPIVVDEVIDEIFDQSRAQAENRSVMLAHQRSSEAVIRGDRRHIEAALRNLIQNAIA
                     YSDAGARVAVATRVVHDATGEWVEIAVSDNGIGIAEADQPRVFERFFRVDYGRSRANG
                     GTGLGLSIVKHIASAHGGTVTLWSRLGQGSTFTLRLPAHQPDHHAEGTAA"
BASE COUNT          608 a         1180 c         1158 g          549 t
ORIGIN      
        1 ttgcgctccg tccttcgttg gattgtgttg atcttttcgg gttcacttgc gtttgtcaac
       61 acgttcacag atatttccca aacaaaccac agtggggtct gccgtgcgac gttccggcag
      121 gtggcgaggg ctccatcgaa cgcccaccga actatccgtg gcctgcgaag gttcgaaggc
      181 cacggatagt tgggcggggg ttggaacacc ggacgtggtg gtgcgcgcgc agcagccggg
      241 ccgtcgatca ccatttcggg ttccgattcg ttaggctgac cccacctgtc gggacgacca
      301 cgaggagagt tcacatggcc ggcacgctgc gccgcttctg cgtcaccctg tgtgttccgc
      361 tcgcgttggc cctgaccggc tgcattgcgc agtttcagac ctcgcagccc tacaccccgg
      421 gcgagggcgt caacctccag gtggggcagg tgaaggtgcg caacctgatg gtgatcgccg
      481 acgcctcggg gcagggcgtg ctcagcgcgt ccgcggtcag ctacgccgac gacacgctca
      541 ccgcagtgag cggcaccccg cacaaggcgg acgggactcc cggcagtgcg ctggtggtga
      601 ccagcaccgg actcccgctg gcaatgccgg ccaacacgct gttcatactg accgactcac
      661 ccctgcgcgt ggcggtcact tcgccggacc tcaagccggg cctgctcgcc gagatcactc
      721 tcaccttcgc caaggcgggc gacgtgaagg cgctggcgcc cgtggtgtcg tcctccgaac
      781 ccgagtactc gggcatcacc gtcggcagct gagcgggtca tcgcacgcct gatgccgggt
      841 gaagctgagc agagcccgga cacaccctta ctccgttgac tgggtttatc gacgggcgcc
      901 caggacgggc tcgaccagag gcctacccgt catccagggc ttgccccggg atgaggacgc
      961 aagcaaggtc ccggcatgcg ccgggatgac ggaaacgttg agcgacctca gacgcgccga
     1021 gggtctcacc agaggcgtta cttgtcatcc cgggctcgcc ccgggatctc gacgcccaca
     1081 aagatcccgg catgcgccgg gatgacggaa acagacagac gttgggatga cggaaacaga
     1141 cgtcgggatg gcggaaacag acgtcgggat ggcggacaca gacgttggga tgacggaaac
     1201 agacgttggg atgacggaaa cagacgtcgg gatgaccgaa acagaccttg ggatgacggg
     1261 gttccggcca ttcaggcgac ccccgaacac cccccgcgac ccttggttta gcttgtcgaa
     1321 accccactgg caccccgtct caacaagctc gaccggcgac ggaacagcgg cccactaggc
     1381 ttcgaacttg tagcccagcc cacgcacggt gaccagcagt tgcggctcgg ccgggtcggt
     1441 ctcgaccttg gcccgcagcc gtttcacgtg cacgtcgagg gtcttggtgt cgccgacgta
     1501 atcggcaccc caaatacggt cgatcagctg cccccgggtc atcacccgtc cggcgttgcg
     1561 cagcaggatc tcgagcaact cgaactcctt gagggcaagt ttcaccggct cgccgcgcac
     1621 ccacacctcg tgccgctcga cgtccatacg cacggcgtcg acctcgagca cgtccggctc
     1681 gagctcgaag tcttggccgc ggcgcagcac tgcccggatg cgcgcgacca actcgcggtg
     1741 gctgaacggc ttggtgacat agtcgtcggc accaagttcg aggcccacca ccttgtcgat
     1801 ctcgtcgtcg cgggcggtga ccatgatcac cggcaccgaa ctgcgtgccc gcagccggcg
     1861 gcacacctcg gtgccgggca ggccgggcat catcagatcg agcagcacga tatcggcgcc
     1921 accgcgctcg aactctgcca gcccgatggt gccgtcggca gcctcgacca cgtcgaagcc
     1981 ctctttgcgt aacaggtagc cgaccgtttc gcggtaggcg tcctcgtcct cgatcaatag
     2041 cactctggtc atgcggccgt cccttcggcg tgatggtcgg gttggtgcgc gggcagccgc
     2101 agcgtgaagg tcgacccttg gcccaggcgg ctccacagcg tgaccgtgcc gccgtgcgcg
     2161 gacgcgatgt gcttgacgat cgacaacccc aggccggtgc cgccgttggc ccggctgcgc
     2221 ccgtagtcga cccggaagaa gcgctcgaac acgcgaggct gatcggcctc ggcgatgccg
     2281 atgccgttgt cggagactgc gatctcgacc cactcgccgg tggcgtcgtg caccacacgc
     2341 gtggcgaccg ccacccgggc accggcatcc gagtaggcga tcgcgttctg gatcaggttg
     2401 cgcagcgccg cctcgatgtg acggcgatca cccctgatca cggcctcgga cgagcgctgg
     2461 tgcgccagca tcacgctgcg attctcagcc tgcgcgcggg actggtcgaa gatctcgtcg
     2521 atcacctcgt ccaccacaat cggaacggct gcgagcaacg ggtcggtgga ctgcagccgt
     2581 gacagggcga tgatctggga gatcagctca tccagccggg cggtctcggt cgccatccgg
     2641 ccggcgaagt gacgcacggc gtccgcatcg tccgcggagg ccgcaacggc ttcggcgagc
     2701 agtgagatcg caccgatcgg cgtcttcagc tcgtgggtga tgttggccac gaagtcgcgg
     2761 cgggtctcgt ccacgcgcag ggcctcggag cggtcgtccg cgaccagcaa caccaggccg
     2821 tcacccaagg gggcgacgcg aacggcgagg cggcgggccg gcaggccaag accggtcggc
     2881 acatcgatgt tcaacgcctc gggctcctcg tcgcgacggg cttcgcgaac cagttcgagc
     2941 acctcgggct gcgcgacgcg gctgccccgc accaccccgg aacttgcagc gctgtgggat
     3001 tgggcgagca actcgtcgtg cgggccgagt accgccgccg ccccgcgcag atgctcgact
     3061 accagccgca cctggggcgc cagcagtggc cgtggttcct gcgcgacgga ggcctgctgc
     3121 cgggcgcgcc gcagggtcat ggcgaagagg gtgaccagaa cgcccagaac gaccccgagg
     3181 gcacaggcga cgagcgggtt cacgcctgga ttctagagcg ttgccccggg gccggatcga
     3241 ccgggggcga gtgacccgtg ccagagttca ctgactgttc acctcagctt cgccggaggg
     3301 gtccacgctg ccacgacccg gcaatagggt ggacgagccg ccagcccggc aaccctgatc
     3361 gtgcagcgaa ggcgaaagag gaaagcatgc gagagaccta ccgtgaagac ctcaacggtg
     3421 tggtggaatc cctggtcagc atgaccgaac gggtccgcaa cgccgtccgg ctcagcacgc
     3481 aggcgttgct caccg
//
LOCUS       DAOSUG010000334         3470 bp    DNA     linear   ENV 10-JUN-2024
DEFINITION  MAG TPA_asm: Micropruina sp. isolate AMR_MDS_2195
            NODE_11115_length_3470_cov_4.712445, whole genome shotgun sequence.
ACCESSION   DAOSUG010000334 DAOSUG010000000
VERSION     DAOSUG010000334.1
DBLINK      BioProject: PRJNA925477
            BioSample: SAMN32864262
            Sequence Read Archive: SRR4236673
KEYWORDS    WGS; ENV; Metagenome Assembled Genome; MAG; Third Party Data; TPA;
            TPA:assembly.
SOURCE      Micropruina sp. (wastewater metagenome)
  ORGANISM  Micropruina sp.
            Bacteria; Bacillati; Actinomycetota; Actinomycetes;
            Propionibacteriales; Nocardioidaceae; Micropruina.
REFERENCE   1  (bases 1 to 3470)
  AUTHORS   Abdulkadir,N., Saraiva,J.P., Zhang,J., Stolte,S., Gillor,O.,
            Harms,H. and Rocha,U.
  TITLE     Genome-centric analyses of 165 metagenomes show that mobile genetic
            elements are crucial for the transmission of antimicrobial
            resistance genes to pathogens in activated sludge and wastewater
  JOURNAL   Microbiol Spectr 12 (3), e0291823 (2024)
   PUBMED   38289113
REFERENCE   2  (bases 1 to 3470)
  AUTHORS   Abdulkadir,N.
  TITLE     Direct Submission
  JOURNAL   Submitted (24-MAR-2023) Environmental Microbiology, Helmholtz
            Center for Environmental Research -UFZ, Permoserstr 15, Leipzig
            04318, Germany
COMMENT     The annotation was added by the NCBI Prokaryotic Genome Annotation
            Pipeline (PGAP). Information about PGAP can be found here:
            https://www.ncbi.nlm.nih.gov/genome/annotation_prok/
            
            ##Genome-Assembly-Data-START##
            Assembly Method        :: SPAdes v. 3.11.1
            Genome Representation  :: Full
            Expected Final Version :: Yes
            Genome Coverage        :: Not Applicable
            Sequencing Technology  :: Illumina MiSeq
            ##Genome-Assembly-Data-END##
            
            ##Genome-Annotation-Data-START##
            Annotation Provider               :: NCBI
            Annotation Date                   :: 05/04/2023 04:57:10
            Annotation Pipeline               :: NCBI Prokaryotic Genome
                                                 Annotation Pipeline (PGAP)
            Annotation Method                 :: Best-placed reference protein
                                                 set; GeneMarkS-2+
            Annotation Software revision      :: 6.5
            Features Annotated                :: Gene; CDS; rRNA; tRNA; ncRNA
            Genes (total)                     :: 2,776
            CDSs (total)                      :: 2,750
            Genes (coding)                    :: 2,714
            CDSs (with protein)               :: 2,714
            Genes (RNA)                       :: 26
            tRNAs                             :: 24
            ncRNAs                            :: 2
            Pseudo Genes (total)              :: 36
            CDSs (without protein)            :: 36
            Pseudo Genes (ambiguous residues) :: 0 of 36
            Pseudo Genes (frameshifted)       :: 2 of 36
            Pseudo Genes (incomplete)         :: 32 of 36
            Pseudo Genes (internal stop)      :: 2 of 36
            ##Genome-Annotation-Data-END##
FEATURES             Location/Qualifiers
     source          1..3470
                     /organism="Micropruina sp."
                     /mol_type="genomic DNA"
                     /submitter_seqid="NODE_11115_length_3470_cov_4.712445"
                     /isolate="AMR_MDS_2195"
                     /isolation_source="Activated sludge of municipal
                     wastewater treatment plants"
                     /db_xref="taxon:2737536"
                     /environmental_sample
                     /geo_loc_name="Canada"
                     /lat_lon="49.25 N 123.1 W"
                     /collection_date="2015-06-16"
                     /metagenome_source="wastewater metagenome"
                     /note="metagenomic"
     gene            complement(297..770)
                     /locus_tag="PLF56_10520"
     CDS             complement(297..770)
                     /locus_tag="PLF56_10520"
                     /inference="COORDINATES: ab initio
                     prediction:GeneMarkS-2+"
                     /note="Derived by automated computational analysis using
                     gene prediction method: GeneMarkS-2+.
                     GO_function: GO:0003677 - DNA binding [Evidence IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="helix-turn-helix transcriptional regulator"
                     /protein_id="HOQ54034.1"
                     /translation="MSSLDFRNVDASPEDPVEDWPYEALVTAMERGYLADWSRIAHSI
                     KRAPWGKTARAVEAYAEYGEHDIAALLTETIRIARISACAAAADAVAGQVRSAVTRSG
                     LTAGEFALEVGTSGSRMSTYMAGKVAPSAPLLQRIVWTADRLQKAAGESESTSRS"
     gene            complement(772..1395)
                     /locus_tag="PLF56_10525"
     CDS             complement(772..1395)
                     /locus_tag="PLF56_10525"
                     /inference="COORDINATES: ab initio
                     prediction:GeneMarkS-2+"
                     /note="Derived by automated computational analysis using
                     gene prediction method: GeneMarkS-2+."
                     /codon_start=1
                     /transl_table=11
                     /product="hypothetical protein"
                     /protein_id="HOQ54035.1"
                     /translation="MPDAVLVGGTVSALYAGHRVSFDHDHVLRDLADRYQAVLEAVEA
                     TEGWATSVRASKPPVTIMGRLGGVEAGLRQVRRTTALEVAEFELDDGRRIRIPTAAEA
                     LRVKAYLIVQRNQVRDYLDVAAMTDRYGVELAGSILRGIDGYYRDRSEMDGSVSSMLA
                     LRLADPQPRDERTIHELASYKGLAPRWTDWGEVRQVCAQLAQEMLDQ"
     gene            complement(1688..>2642)
                     /locus_tag="PLF56_10530"
     CDS             complement(1688..>2642)
                     /locus_tag="PLF56_10530"
                     /EC_number="5.4.4.2"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_017933142.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_component: GO:0005737 - cytoplasm [Evidence IEA];
                     GO_function: GO:0008909 - isochorismate synthase activity
                     [Evidence IEA];
                     GO_process: GO:0009234 - menaquinone biosynthetic process
                     [Evidence IEA]"
                     /codon_start=2
                     /transl_table=11
                     /product="isochorismate synthase"
                     /protein_id="HOQ54036.1"
                     /translation="VIPANAGISPSAPDGDAGTPLVELVETPEGTNLRSAASPVRAPR
                     DVRLTQDPAAEDGWKRRVAQAVHRIETNGLEKVVLGRSVTVQAADPIDVRHLVDALAR
                     DYPSCWTYSIDGLVGASPEMLIRRERGLATSRILAGTIRRSGSEETDLKLASALARSS
                     KNLGEHELAVESVAQALAPLCSGMNVPESPYVLELPNVLHLATDVTAVAHKKASSLRL
                     AAALHPSAAVCGTPTDVARAAIAELEGLDRGRYSGPVGWIDSRGDGEWAIALRCGILN
                     ADDPRRMELFAGCGIVEGSDPEEELAETQAKLVPMLDALLG"
     assembly_gap    2643..2742
                     /estimated_length=100
                     /gap_type="within scaffold"
                     /linkage_evidence="paired-ends"
     gene            complement(<2743..3221)
                     /locus_tag="PLF56_10535"
     CDS             complement(<2743..3221)
                     /locus_tag="PLF56_10535"
                     /inference="COORDINATES: ab initio
                     prediction:GeneMarkS-2+"
                     /note="Derived by automated computational analysis using
                     gene prediction method: GeneMarkS-2+."
                     /codon_start=1
                     /transl_table=11
                     /product="hypothetical protein"
                     /protein_id="HOQ54037.1"
                     /translation="MAKYLVDGSSVAWIRRGEGMVGLGCHACTDVSGPAEADAWWSAF
                     VEGLEHESEVHGVAGSGPLAFGSFTFDPGHTEAASRLIVPKVIIGRRQGQAWVTIVGD
                     EPLASVIPANASPVIPANAGISLVPANPSPVIPASSPVIAASSPVIAASSPVIAASSP
                     "
BASE COUNT          605 a         1092 c         1118 g          555 t
ORIGIN      
        1 catgagagtg atccttccaa cgaaccagcc ctccggacac gccggggcgg ttcaatatga
       61 caatcggtga aataggccag attggggcgt atcaaggttg aggaagctca gcgtcgaaag
      121 tgaggccgaa cctgacaggt ggcgggtggt cattccacct ggtgtgagta ggccatcaga
      181 gccgggtcga acagaggcag acgcgattgc gatagctgtg ttaggtcacg ctgccacgac
      241 gcagcctgac gtgacaaacc agtcagaaca acacagtgat ctgcaagctc ggccgtttat
      301 gaccgagaag ttgactcaga ttcgccagca gccttctgca agcgatccgc ggtccacaca
      361 atgcgctgca gcaatggcgc tgacggggcg acctttcccg ccatgtaggt cgacatccga
      421 gaccctgacg tgccgacttc gagcgcaaac tcgccagccg tgagtccgct gcgtgtgacc
      481 gctgacctga cctggccggc caccgcatca gctgcggcgg cacaggccga gatgcgggcg
      541 atgcggatgg tttcggtgag cagggctgct atgtcgtgct cgccatattc ggcatatgcc
      601 tcgacagcgc gcgcagtctt tccccagggg gctcgcttga tgctatgagc gatccgcgac
      661 cagtcagcta ggtatccccg ctccatggcg gtgacgagcg cctcgtacgg ccaatcttca
      721 accgggtcct ccggtgacgc atcgacattg cgaaaatcca gactcgacat ctcactgatc
      781 cagcatttcc tgcgccaact gggcgcacac ctgccggacc tcaccccagt cagtccatcg
      841 gggcgccaat cccttgtagg aggcaagttc gtggatggtg cgttcgtccc gtggctgagg
      901 gtcggcaagg cgcaatgcca acatcgacga gacggagccg tccatctccg agcggtcccg
      961 gtagtacccg tcgatacccc gaaggatcga acccgccaac tccacgccgt accggtctgt
     1021 catcgcagcg acatcaaggt agtcgcgcac ctggttgcgt tgcacgatca ggtacgcctt
     1081 gacgcgaagg gcttcggcag cggttgggat ccggatgcgg cgtccgtcgt ccaactcgaa
     1141 ctccgccact tccaacgcgg tggtgcggcg cacctgtcgc aagccagcct ccaccccacc
     1201 caggcgcccc atgatcgtta cgggaggctt cgatgcacga acgctggtgg cccaaccttc
     1261 ggtggcctca acagcctcca aaacggcttg gtagcggtcg gccaggtcgc gcagcacatg
     1321 gtcgtggtcg aacgagaccc gatgaccggc gtatagagcg ctgacggttc ctccgacaag
     1381 caccgcgtcg ggcacaacct gctgcaggcg agcagcgctg tcgaagacag cttccatccc
     1441 ttctggaacc cccagaggtt cgaagtgcat tgggtagccg agtgagagtt cgggttcgtc
     1501 aagtggctca ctgccgaacc gtccggcacg ttggcgaggc tggccactcg acatactcac
     1561 agggcaatcg tatcagatgt ggtaactcag gtaagtcgga gccatcctgg ccccgtttcc
     1621 ggtggaagta accacagggg gagtcatgtt cggccagacc cgcgccattc gcggagacgc
     1681 acgatcgtca gccgagcagc gcgtccagca tcggcaccag cttggcctgg gtctcggcca
     1741 gttcttcttc tggatctgag ccctcgacga tgccgcagcc cgcgaacagt tccatgcgcc
     1801 gaggatcgtc cgcattcagg attccgcagc gcaaggcgat cgcccattcc ccgtccccgc
     1861 ggctgtcgat ccagccgacg gggccggagt agcggccccg gtcgagtcct tcgagttcgg
     1921 cgatcgccgc ccgggccacg tcggtcggtg tgccgcacac ggcggcggac gggtgcaggg
     1981 ctgccgccag ccgcagcgag gacgccttct tgtgggccac cgcggtcacg tcggtggcca
     2041 ggtgcagcac gttgggcagt tccagcacgt agggcgattc gggcacgttc atgcccgaac
     2101 acagaggtgc gagggcttgc gccactgatt cgacggccag ttcgtgctcg ccgaggttct
     2161 tcgacgaccg ggccagcgcc gaggccagct tcaggtcggt ctcctccgac cctgagcgtc
     2221 ggatcgtccc ggcgaggatc cgcgacgtgg ccagcccacg ttcgcgccgg atcagcatct
     2281 cgggggaggc gccgaccagg ccgtcgatcg agtacgtcca gcaactcggg tagtcgcggg
     2341 ccagtgcgtc gaccaggtgc cgcacgtcga tcgggtcggc ggcctggacg gtcaccgatc
     2401 ggcccagcac caccttttcc aaaccgttgg tttcgatgcg gtggacggcc tgggccaccc
     2461 ggcgcttcca gccatcctcc gcggctgggt cctgggtgag ccggacgtcg cggggtgctc
     2521 ggacggggga ggcggcggat cggaggttcg tgccctccgg ggtctcgaca agctcgacca
     2581 gcggggtacc agcgtcaccg tcaggcgcgc tgggggagat cccggcgttc gccgggatga
     2641 cgnnnnnnnn nnnnnnnnnn nnnnnnnnnn nnnnnnnnnn nnnnnnnnnn nnnnnnnnnn
     2701 nnnnnnnnnn nnnnnnnnnn nnnnnnnnnn nnnnnnnnnn nnggggaact ggccgcgatg
     2761 acgggggaac tggccgcgat gacgggggaa ctggccgcga tgacggggga actggccggg
     2821 atgacggggg acgggttcgc ggggacgagg gagattccgg cgttcgccgg aatgacgggg
     2881 gaggcgttcg ccgggatgac ggaagcaagg ggctcatccc ccacgatcgt cacccacgcc
     2941 tggccctgcc ggcggccgat gatcaccttg gggacgatca gccgcgatgc ggcctcggtg
     3001 tgcccgggat cgaaggtgaa cgagccgaat gccaggggtc ccgaacccgc cactccgtgc
     3061 acctcggact cgtgctccaa gccctcaacg aaagcgctcc accaggcgtc cgcctcggcc
     3121 gggccggaca cgtccgtgca cgcgtgacag cccaaaccga ccatgccctc gccgcgccgg
     3181 atccacgcca ccgatgagcc gtccactagg tattttgcca acgatcctgg gtcgttaatc
     3241 gcgatggtgc gggcaaaagt cgaggcggcc ggcggcgcag agatcaccgg ggaagcatag
     3301 tcaggtcaca ccctgacctc gaatggctgt cgtgtcattt cgtgcctaga ctggcgaggc
     3361 ccaccccggc aaaaacaacg tgacgacgag gagcgatctg tgtccgacga gcaagcggac
     3421 gtcgtcgtcg tcggggccgg ccccgccggc gcgacgacgg cgtgctggct
//
LOCUS       DAOSUG010000335         3470 bp    DNA     linear   ENV 10-JUN-2024
DEFINITION  MAG TPA_asm: Micropruina sp. isolate AMR_MDS_2195
            NODE_11116_length_3470_cov_3.816984, whole genome shotgun sequence.
ACCESSION   DAOSUG010000335 DAOSUG010000000
VERSION     DAOSUG010000335.1
DBLINK      BioProject: PRJNA925477
            BioSample: SAMN32864262
            Sequence Read Archive: SRR4236673
KEYWORDS    WGS; ENV; Metagenome Assembled Genome; MAG; Third Party Data; TPA;
            TPA:assembly.
SOURCE      Micropruina sp. (wastewater metagenome)
  ORGANISM  Micropruina sp.
            Bacteria; Bacillati; Actinomycetota; Actinomycetes;
            Propionibacteriales; Nocardioidaceae; Micropruina.
REFERENCE   1  (bases 1 to 3470)
  AUTHORS   Abdulkadir,N., Saraiva,J.P., Zhang,J., Stolte,S., Gillor,O.,
            Harms,H. and Rocha,U.
  TITLE     Genome-centric analyses of 165 metagenomes show that mobile genetic
            elements are crucial for the transmission of antimicrobial
            resistance genes to pathogens in activated sludge and wastewater
  JOURNAL   Microbiol Spectr 12 (3), e0291823 (2024)
   PUBMED   38289113
REFERENCE   2  (bases 1 to 3470)
  AUTHORS   Abdulkadir,N.
  TITLE     Direct Submission
  JOURNAL   Submitted (24-MAR-2023) Environmental Microbiology, Helmholtz
            Center for Environmental Research -UFZ, Permoserstr 15, Leipzig
            04318, Germany
COMMENT     The annotation was added by the NCBI Prokaryotic Genome Annotation
            Pipeline (PGAP). Information about PGAP can be found here:
            https://www.ncbi.nlm.nih.gov/genome/annotation_prok/
            
            ##Genome-Assembly-Data-START##
            Assembly Method        :: SPAdes v. 3.11.1
            Genome Representation  :: Full
            Expected Final Version :: Yes
            Genome Coverage        :: Not Applicable
            Sequencing Technology  :: Illumina MiSeq
            ##Genome-Assembly-Data-END##
            
            ##Genome-Annotation-Data-START##
            Annotation Provider               :: NCBI
            Annotation Date                   :: 05/04/2023 04:57:10
            Annotation Pipeline               :: NCBI Prokaryotic Genome
                                                 Annotation Pipeline (PGAP)
            Annotation Method                 :: Best-placed reference protein
                                                 set; GeneMarkS-2+
            Annotation Software revision      :: 6.5
            Features Annotated                :: Gene; CDS; rRNA; tRNA; ncRNA
            Genes (total)                     :: 2,776
            CDSs (total)                      :: 2,750
            Genes (coding)                    :: 2,714
            CDSs (with protein)               :: 2,714
            Genes (RNA)                       :: 26
            tRNAs                             :: 24
            ncRNAs                            :: 2
            Pseudo Genes (total)              :: 36
            CDSs (without protein)            :: 36
            Pseudo Genes (ambiguous residues) :: 0 of 36
            Pseudo Genes (frameshifted)       :: 2 of 36
            Pseudo Genes (incomplete)         :: 32 of 36
            Pseudo Genes (internal stop)      :: 2 of 36
            ##Genome-Annotation-Data-END##
FEATURES             Location/Qualifiers
     source          1..3470
                     /organism="Micropruina sp."
                     /mol_type="genomic DNA"
                     /submitter_seqid="NODE_11116_length_3470_cov_3.816984"
                     /isolate="AMR_MDS_2195"
                     /isolation_source="Activated sludge of municipal
                     wastewater treatment plants"
                     /db_xref="taxon:2737536"
                     /environmental_sample
                     /geo_loc_name="Canada"
                     /lat_lon="49.25 N 123.1 W"
                     /collection_date="2015-06-16"
                     /metagenome_source="wastewater metagenome"
                     /note="metagenomic"
     gene            complement(164..550)
                     /locus_tag="PLF56_10540"
     CDS             complement(164..550)
                     /locus_tag="PLF56_10540"
                     /inference="COORDINATES: ab initio
                     prediction:GeneMarkS-2+"
                     /note="Derived by automated computational analysis using
                     gene prediction method: GeneMarkS-2+."
                     /codon_start=1
                     /transl_table=11
                     /product="hypothetical protein"
                     /protein_id="HOQ54038.1"
                     /translation="MTAENGFHDSDPFADGFGDSEHSTQPLAVALTGPPFGWLAAGVL
                     MAIVGGAVAWLVGSLAVAAAGWLLAGPVGLTLVAWYVNRDNHARVSGVYQAPGWTTLA
                     YAAGVAICVLGVLAAALRVAEGVGRL"
     gene            complement(547..696)
                     /locus_tag="PLF56_10545"
     CDS             complement(547..696)
                     /locus_tag="PLF56_10545"
                     /inference="COORDINATES: ab initio
                     prediction:GeneMarkS-2+"
                     /note="Derived by automated computational analysis using
                     gene prediction method: GeneMarkS-2+."
                     /codon_start=1
                     /transl_table=11
                     /product="hypothetical protein"
                     /protein_id="HOQ54039.1"
                     /translation="MTLPPISGYIGGRIGVDEAMRHLDESVATLTELGAPLLQWLLRI
                     LGVNG"
     gene            complement(1240..1947)
                     /locus_tag="PLF56_10550"
     CDS             complement(1240..1947)
                     /locus_tag="PLF56_10550"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_014845826.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_function: GO:0005507 - copper ion binding [Evidence
                     IEA];
                     GO_process: GO:0055070 - copper ion homeostasis [Evidence
                     IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="copper homeostasis protein CutC"
                     /protein_id="HOQ54040.1"
                     /translation="MSGLLEVIALHAADAERAEAGGADRIELCGSLEDGGMSPEPALV
                     DKVRAATSVQLRVMVRLRAGYGTDGGEAVRLRGLIASYLDAGADGLVLGFLNGLGEVD
                     PQVVTELIGDGDWPWTFHRAIDSCLYPDRGWAMLARLPRLDQVLTAGSARGLEHGLDD
                     VLARAKADPWQASVIMAGGGLHPDHVPWLARAGVRAFHIGGPARPQGSYKAYVDPELV
                     GAWRRLIDTEIRHAQGG"
     gene            complement(2305..>3470)
                     /locus_tag="PLF56_10555"
     CDS             complement(2305..>3470)
                     /locus_tag="PLF56_10555"
                     /inference="COORDINATES: protein motif:HMM:NF019310.2"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_function: GO:0022857 - transmembrane transporter
                     activity [Evidence IEA];
                     GO_process: GO:0055085 - transmembrane transport [Evidence
                     IEA]"
                     /codon_start=3
                     /transl_table=11
                     /product="MFS transporter"
                     /protein_id="HOQ54041.1"
                     /translation="FLVGSVLAGLAWNMASLIGFRVVQGLGAGAVAPMAMTIVGDIYT
                     VAERAIVQGYIASVWAIASVVGPALGGIFSQFTSWRWIFLVNIPLCLLAGWALLRFYH
                     ERVESKRHRIDYLGAGLLTLGLTAVILALLEGGNAWSWTSWQSLGSFVLGIAALVAFV
                     TVERRAPEPVLDLRIVGRPLIATATLIALGVGALLIGVTSFVPTYLENSLGVVPLVSG
                     MAVAALTLGWPLAASLAGRLYLRFGYRTTVLLGSSIAAAGTVALALTGPAPNPWTVGA
                     VCLVIGFGLGWTAAPSLIAVQASVDWDERGVVTGVNVFARSAGSAIGVALYGAIATNV
                     IAAGKGEHDYATIVTASTWVFVAVAVTAVGMLVAALGLPRGGVHAAAYSPPSA"
BASE COUNT          654 a         1222 c         1131 g          463 t
ORIGIN      
        1 agcaccagga cgtccgcctg ccccgaggca gcgacacagg cggcgaatcg atcggcggcc
       61 ttgtcagtgg cgtttgcgct ggcgggccgg gacgtcaggg gcgtcaggca caccgaaccg
      121 atcaggatca cccacaggcc gaccgccgat gccagcctct gcctcacagc cgacccaccc
      181 cttcagctac ccgcagcgcg gcggccaaca cgccgagcac acagatggct acgcccgccg
      241 cgtaggcgag tgtggtccac ccgggggcct ggtaaacgcc ggaaacccgg gcgtggttgt
      301 cgcggttgac gtaccaggcg acaagggtga gccccaccgg tccggccaac agccagccgg
      361 ccgcagcgac tgccagggat ccgacgagcc atgcgacagc acccccgaca atggccatga
      421 gcacccccgc ggcgagccac ccgaacggcg gcccggtgag ggcgaccgcg agcggctggg
      481 tggaatgctc agagtcgccg aatccgtcgg cgaaagggtc cgaatcgtgg aagccgttct
      541 cagccgtcat ccgttcactc ccagaatgcg caggagccat tgcagcaacg gggctccgag
      601 ttcggtcaac gtggccacgc tctcatccag atgccgcatg gcttcgtcga cgccgatcct
      661 gccaccgata tatccggaga tcggcggcag ggtcaatgac acaattgtca agacccatgg
      721 catcggggac cgtccacccg ttcgaacgac aaccaccacg catgcaatcg acgctgccag
      781 gcacgccatg atgggcagca caattgccca cgaccagaac gatgcgtggg cgagaccgtc
      841 ctccgatggg cctaggccgg acaccatcga atacaccgcc cacccgaaga gtgcgctgca
      901 agcgacgacc acacccggta ggacgacctg acgccctgtt gtggtcatcg gggagggact
      961 caccgctatg gacagcgaag gttcgctgga cgcatcatca aaggggtcgt cactgaacgg
     1021 atcgtcaccc gcgaccattg atccccctca tcaaatgctc gttcggtgaa cggaatgctg
     1081 tgcagaacca ttcgccgcgt ccacggaata attcatgccg caccgagggc ggctgccttc
     1141 ggtaggcacg ctacaccgag tcgcccgccg gctcaggcgt ctttcaccag aatcgatgcc
     1201 gccacgtgtg gatatgctga aagcagcggc aggcatttct cacccgccct gggcgtgcct
     1261 gatctcggtg tcgatcagac gtcgccaggc acccaccagt tcggggtcga cgtaggcctt
     1321 gtacgacccc tgcggacgag ccggcccacc gatgtggaac gcacgcaccc cggcgcgggc
     1381 cagccagggc acgtgatcgg ggtgcagtcc gccgccggcc atgatcaccg acgcctgcca
     1441 cgggtcggct ttcgctctgg cgagcacatc gtcgaggccg tgttccagcc cgcgggccga
     1501 tccggcggtg agcacctgat caagcctcgg cagccgcgca agcatggccc agccacgatc
     1561 ggggtagagg caggaatcga tcgcgcggtg gaacgtccag ggccagtcgc cgtcgccgat
     1621 cagctcggtc accacctgag ggtcgacttc accgagtccg ttgagaaagc cgagcaccag
     1681 gccgtccgcg ccggcgtcca ggtagctggc gatcagaccc ctcaaccgga cggcctcgcc
     1741 gccgtcggtg ccgtagccgg cccggagacg caccatcacc cgcagctgca cggacgtggc
     1801 cgcgcgcacc ttgtcgacca gcgccggctc aggcgacatg ccgccgtctt cgagggaccc
     1861 gcacagttcg atgcggtcgg cgccacccgc ttcggcacgt tcggcatcgg ccgcgtgcaa
     1921 tgcgatcacc tcgagcagcc cgctcatgcc cccatcatga gcgattgccc ttgtggctga
     1981 gcgcgagaca cgcacgatca gctgatcgaa ctgtgcagcc gtcgctccag caccgcacgg
     2041 gtggacgacc tgcgtgcgac tgggccgttc caccccgtcc cgatcgagag ccaaggaagc
     2101 gcagatcgtc gccggtcgag ccacgaatgt gctgatcacc ctcaacttcc gttggtcgag
     2161 cttctcgaca accctgcaca cgagcgtctc aacaagtgcg aacggcatag gccaacgtgt
     2221 cgctgaacga cgagaccctc gggagtgggg gttccgacgc tcgaccaacg cactgtgcgt
     2281 tgagcagcgc ttccctgagc tgactcacgc gctgggcggt gagtaggcgg cggcatgcac
     2341 cccgccccgg ggcaggccca gggccgcgac cagcatgccg accgcggtta ccgccaccgc
     2401 gacgaacacc caggtggacg cggtcacgat cgtcgcgtag tcgtgttcgc ccttgccggc
     2461 cgcgatcaca ttggtggcga tggcgccgta cagtgcgacg ccgatcgcgc tgcccgccga
     2521 tcgggcgaac acgttgacgc cggtcacgac gccgcgctcg tcccagtcga ccgaggcctg
     2581 cacggcgatc agcgagggag cggccgtcca gcccaggccg aagccgatca ccaggcagac
     2641 ggccccgacc gtccacggat tcggggcggg gccggtcagc gcgagcgcga ccgtgccggc
     2701 cgccgcgatc gagctgccga gcagaacggt ggtgcggtag ccgaatcgca ggtacagccg
     2761 tccggccagc gaggcggcca ggggccagcc gagggtgagc gcagcgacag ccatgcccga
     2821 gaccaacggc accacgccga gcgagttctc gagataggtg ggcacgaagc tggtgacccc
     2881 gatcagcagt gcgccgactc cgagcgcgat cagagtcgcg gtggcgatca gcggtcgccc
     2941 cacgatgcgt agatcgagca ccggttcggg cgctcgtcgc tcgaccgtga cgaacgcgac
     3001 cagggcagcg atgcccagga cgaagctgcc caggctctgc cacgaggtcc acgaccaggc
     3061 gttgccgccc tcgagcaggg ccaggatcac cgcggtcagg cccagggtga gcaggccggc
     3121 gccgagatag tcgatgcggt ggcgcttgct ctcgacccgc tcgtggtaga accggagcag
     3181 cgcccagccc gccagcagac acagcggaat gttgaccaga aagatccacc gccacgaggt
     3241 gaactggctg aagatgccgc ccagtgccgg gcccaccacg gacgcgatgg cccacacact
     3301 ggcgatgtag ccctgcacga tggcgcgctc ggcgacggtg tagatgtcgc cgacgatcgt
     3361 catggccatc ggggcgacgg cgccggcacc aagcccttgc accacccgga agccgatcag
     3421 cgaggccatg ttccaggcca gcccggccag caccgagccg accaggaaaa
//
LOCUS       DAOSUG010000336         3466 bp    DNA     linear   ENV 10-JUN-2024
DEFINITION  MAG TPA_asm: Micropruina sp. isolate AMR_MDS_2195
            NODE_11133_length_3466_cov_3.153621, whole genome shotgun sequence.
ACCESSION   DAOSUG010000336 DAOSUG010000000
VERSION     DAOSUG010000336.1
DBLINK      BioProject: PRJNA925477
            BioSample: SAMN32864262
            Sequence Read Archive: SRR4236673
KEYWORDS    WGS; ENV; Metagenome Assembled Genome; MAG; Third Party Data; TPA;
            TPA:assembly.
SOURCE      Micropruina sp. (wastewater metagenome)
  ORGANISM  Micropruina sp.
            Bacteria; Bacillati; Actinomycetota; Actinomycetes;
            Propionibacteriales; Nocardioidaceae; Micropruina.
REFERENCE   1  (bases 1 to 3466)
  AUTHORS   Abdulkadir,N., Saraiva,J.P., Zhang,J., Stolte,S., Gillor,O.,
            Harms,H. and Rocha,U.
  TITLE     Genome-centric analyses of 165 metagenomes show that mobile genetic
            elements are crucial for the transmission of antimicrobial
            resistance genes to pathogens in activated sludge and wastewater
  JOURNAL   Microbiol Spectr 12 (3), e0291823 (2024)
   PUBMED   38289113
REFERENCE   2  (bases 1 to 3466)
  AUTHORS   Abdulkadir,N.
  TITLE     Direct Submission
  JOURNAL   Submitted (24-MAR-2023) Environmental Microbiology, Helmholtz
            Center for Environmental Research -UFZ, Permoserstr 15, Leipzig
            04318, Germany
COMMENT     The annotation was added by the NCBI Prokaryotic Genome Annotation
            Pipeline (PGAP). Information about PGAP can be found here:
            https://www.ncbi.nlm.nih.gov/genome/annotation_prok/
            
            ##Genome-Assembly-Data-START##
            Assembly Method        :: SPAdes v. 3.11.1
            Genome Representation  :: Full
            Expected Final Version :: Yes
            Genome Coverage        :: Not Applicable
            Sequencing Technology  :: Illumina MiSeq
            ##Genome-Assembly-Data-END##
            
            ##Genome-Annotation-Data-START##
            Annotation Provider               :: NCBI
            Annotation Date                   :: 05/04/2023 04:57:10
            Annotation Pipeline               :: NCBI Prokaryotic Genome
                                                 Annotation Pipeline (PGAP)
            Annotation Method                 :: Best-placed reference protein
                                                 set; GeneMarkS-2+
            Annotation Software revision      :: 6.5
            Features Annotated                :: Gene; CDS; rRNA; tRNA; ncRNA
            Genes (total)                     :: 2,776
            CDSs (total)                      :: 2,750
            Genes (coding)                    :: 2,714
            CDSs (with protein)               :: 2,714
            Genes (RNA)                       :: 26
            tRNAs                             :: 24
            ncRNAs                            :: 2
            Pseudo Genes (total)              :: 36
            CDSs (without protein)            :: 36
            Pseudo Genes (ambiguous residues) :: 0 of 36
            Pseudo Genes (frameshifted)       :: 2 of 36
            Pseudo Genes (incomplete)         :: 32 of 36
            Pseudo Genes (internal stop)      :: 2 of 36
            ##Genome-Annotation-Data-END##
FEATURES             Location/Qualifiers
     source          1..3466
                     /organism="Micropruina sp."
                     /mol_type="genomic DNA"
                     /submitter_seqid="NODE_11133_length_3466_cov_3.153621"
                     /isolate="AMR_MDS_2195"
                     /isolation_source="Activated sludge of municipal
                     wastewater treatment plants"
                     /db_xref="taxon:2737536"
                     /environmental_sample
                     /geo_loc_name="Canada"
                     /lat_lon="49.25 N 123.1 W"
                     /collection_date="2015-06-16"
                     /metagenome_source="wastewater metagenome"
                     /note="metagenomic"
     gene            <1..874
                     /locus_tag="PLF56_10560"
     CDS             <1..874
                     /locus_tag="PLF56_10560"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_014846507.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=2
                     /transl_table=11
                     /product="DUF885 domain-containing protein"
                     /protein_id="HOQ54042.1"
                     /translation="ESIMAAVCAFASIDDAVRVLDGDAHRKLDGTKALQEWMQTTADT
                     AIADLNGVHFDIPEPVRRIECMIAPTQNGGIYYTGPSDDFSRPGRMWWSVPPGVEQFN
                     TWREKTTVYHEGVPGHHLQIGQAVHNRDELNMWRRLVCWVSGHGEGWALYAERLMQEF
                     GYMDDLGDRFGLLDGQRMRATRVVLDIGVHLGKPAPQQWGGGTWDADKAWELLKANVN
                     MDEKFLRFELNRYLGWPGQAPSYKIGQRLWEQIRAEAAEQAGDAFSLKDFHTRALNLG
                     SLPLSVLRDAVLAG"
     gene            complement(904..2025)
                     /locus_tag="PLF56_10565"
     CDS             complement(904..2025)
                     /locus_tag="PLF56_10565"
                     /inference="COORDINATES: ab initio
                     prediction:GeneMarkS-2+"
                     /note="Derived by automated computational analysis using
                     gene prediction method: GeneMarkS-2+."
                     /codon_start=1
                     /transl_table=11
                     /product="hypothetical protein"
                     /protein_id="HOQ54043.1"
                     /translation="MRATPRAGSPLLAAGIALVAVLVGGCSGPPAGSPTPLPSVAVVA
                     GPPLAGDLSGGTPVQLGAASHWAIRDGRAFVATAPGTVRAVELSTGVQLWQASFEQGE
                     PWDATPTLAVSSDGSEVVAVRTVDSAGAARLDLLRVATASGERVSERLIADPGHRWRV
                     DLPPRVLAVDAETAVLSSDPESGRQSAVVSLLDGSIAWTIDQEAIAATADTVITRLGG
                     QIRTNGAHRWVAARPLGPLLALSAHAVVVAQDSRAVRLDPRSGEVDVLTTKLSEVDAP
                     CVRTATEVVACLGAPGVVAQDLALGRQLWSSRTHADGIAAIADWLYLWHSPNRGDVLD
                     ARTGEVLQQNVDLPAIRYADASGVLLAADDGYRWVPYPR"
     gene            complement(2245..>3466)
                     /locus_tag="PLF56_10570"
     CDS             complement(2245..>3466)
                     /locus_tag="PLF56_10570"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_008355976.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_function: GO:0004222 - metalloendopeptidase activity
                     [Evidence IEA];
                     GO_process: GO:0006508 - proteolysis [Evidence IEA]"
                     /codon_start=2
                     /transl_table=11
                     /product="M3 family metallopeptidase"
                     /protein_id="HOQ54044.1"
                     /translation="VLAELLALRHELAGLLGYANWADYDAEVKMIGSGAAIGEFIDTV
                     SAAALPSGQREIEVLRARAGQDGESVIDVSNWRHYTELIKRERYGVDATEVRRYFDFA
                     KVHDGLLEVTGRLFGLRFTPVEAAEARSWHSDVTSYDVSDAEAGTPLGRIHLDLHPRE
                     RKYNHAACFDMVPGVLGAQLPESVLVCNFSRGLMEHGDVVTLFHEFGHLIHNIVGGHQ
                     RWVRFSGVATEWDFVEAPSQLLEEWAWDAEVLRTFATDAEGTPIPTELVAAMKAADEF
                     GKSFLARTQMFYAALAYTFHVERPADRTERMLQLYRRYSLVAPLPDTHFFAGFGHLEG
                     YTSGYYTYMWSLVIAKDLFSAFDRNDLLAPEVAQRYRDRVLRPGGSRDAADLVESFLA
                     RPYNTDAFAAWLNS"
BASE COUNT          542 a         1208 c         1238 g          478 t
ORIGIN      
        1 cgaaagcatc atggcggcgg tgtgcgcgtt tgcgtccatc gacgatgcgg tgcgggtgct
       61 ggacggcgac gcgcaccgaa agctcgacgg caccaaggcg ctgcaggaat ggatgcagac
      121 caccgccgac acggcgatcg ccgacctgaa cggggtgcac ttcgacattc ccgaaccggt
      181 gcggcggatc gaatgcatga tcgcgcccac ccagaacggc ggcatctact acaccggccc
      241 gtccgacgac ttcagccgtc cgggccgcat gtggtggtcg gtgccgccgg gtgtcgagca
      301 gttcaacacc tggcgcgaga agaccaccgt ctaccacgag ggcgtgccgg gccatcacct
      361 gcagatcggc caggccgtcc acaaccgcga cgagctgaac atgtggcggc ggctggtctg
      421 ctgggtctcg ggccacggcg agggctgggc gctgtacgcc gagcggctga tgcaggagtt
      481 cggctacatg gacgacctgg gcgaccggtt cgggctgctg gacggacagc gcatgcgcgc
      541 cacccgggtg gtgctcgaca tcggcgtgca cctgggcaag ccggcccccc agcagtgggg
      601 tggcggcacc tgggacgccg acaaggcctg ggaactgctc aaggccaacg tgaacatgga
      661 cgagaagttc ctgcgcttcg aactgaaccg ctatctcgga tggccgggtc aggcaccgtc
      721 ctacaagatc gggcagcggc tgtgggagca gattcgcgcc gaggccgccg agcaggccgg
      781 cgacgccttc tcgctgaagg acttccacac ccgggcgctc aacttgggct cgctgccgct
      841 cagcgtgctg cgggacgcgg tgctggccgg ctgagcctgc gggctgagtc gaccggccga
      901 cggttaccgc gggtacggca cccagcgata gccgtcgtcc gcggccagca gcacacccga
      961 ggcatcggcg tagcgaatgg cgggcaggtc cacgttctgc tggagcactt cgccggtgcg
     1021 ggcgtccagc acgtcgcccc ggttgggcga gtgccacagg tagagccagt cggcgatcgc
     1081 ggcgatccca tcggcgtgcg tgcgcgacga ccacagctgt cgcccgagcg ccagatcctg
     1141 tgcgacaacg ccgggtgcgc ccaggcacgc cacgacctcg gtggcggtgc gcacgcacgg
     1201 cgcgtccacc tcgctgagct tcgtggtgag cacgtccact tcgccgctgc gcgggtcgag
     1261 ccggacggcc cgactatcct gagccaccac cacggcgtgg gcggacagcg ccagtagcgg
     1321 ccccaacggc cgcgcggcaa cccacctgtg cgcaccgttg gtacggatct gcccgccgag
     1381 gcgagtgatc actgtgtccg ccgtggcggc gatcgcctcc tggtcgatcg tccaggcgat
     1441 cgaaccgtcg agcaggctga ccaccgcgct ctgccgtccc gactcggggt cggacgacag
     1501 cacggcggtt tcggcgtcca ccgcgagcac gcgcggcgga aggtcgaccc gccaccgatg
     1561 accggggtcg gcgatcaacc gctcggacac gcgctcaccg ctggcggtcg cgacccgcaa
     1621 caggtccagc ctggccgcgc cggcggagtc gacggttcgg acggccacca cctcggagcc
     1681 gtcggagctg accgccaggg tgggtgtggc gtcccagggt tcgccctgct cgaaggacgc
     1741 ctgccagagc tgcacaccgg tactcagctc gacggcacgg acggtgcccg gcgccgtggc
     1801 cacgaaggcc cgcccgtcgc ggatcgccca gtgcgacgcg gcgcccagct gcaccggtgt
     1861 gccgccggac aggtcgccgg ccagcggagg gccggccacc acggcgacgc tgggaagcgg
     1921 ggtcggcgaa cctgcgggcg ggccgctgca cccgccgacc aggacggcga ccagcgcaat
     1981 cccggccgca agcagcggcg accctgcccg tggcgttgcg cgcatgcggt aatcgtcgcg
     2041 ggagcgacgg gtctcgacaa gggcgagcgg agcggacggg gtcggcacgg tcggcacggc
     2101 acggcacggc acggggagcg ctgatcgatg cgcgatcggc tggttgagct ggtcgaaacc
     2161 cctgccgcac aggggctcga cccgctgcac cggcgatgga tcgggtcttc atggcgacgc
     2221 cccggcggcc ccggccccgg cccctcagga gttcagccag gcggcgaagg cgtcggtgtt
     2281 gtacgggcgt gcgagaaagc tctccaccag atcggcggcg tcacggctgc cacccggccg
     2341 cagcacccgg tcgcggtatc gctgcgccac ctcgggcgcc agcaggtcgt tgcggtcgaa
     2401 ggcgctgaac aggtccttgg cgatcaccag cgaccacatg taggtgtagt agccagacgt
     2461 gtagccctcc agatggccga accccgcgaa gaagtgcgtg tcgggcagcg gcgccaccag
     2521 gctgtagcgc cggtagagct gcagcatgcg ttcggtgcga tcggccggac gctccacgtg
     2581 gaacgtgtag gccagcgcgg cgtagaacat ctgggtgcgg gccagaaaac tcttgccgaa
     2641 ctcgtccgcg gccttcatcg ccgccaccag ttccgtcgga atcggggtgc cctcggcgtc
     2701 ggtggcgaag gtgcgcagca cctcggcatc ccacgcccac tcctcgagca gctggctggg
     2761 cgcctcgacg aagtcccact cggtggccac gcccgagaac cgcacccagc gctgatggcc
     2821 gccgacgatg ttgtgaatca ggtggccgaa ctcgtgaaac agggtgacca cgtcgccgtg
     2881 ctccatcagg ccgcgagaga agttgcagac cagcacgctc tcgggcagtt gggcgcccag
     2941 cacgccgggc accatgtcga agcaggccgc gtggttgtac ttgcgctcgc gcgggtgcag
     3001 gtcgaggtga atgcggccca gcggggtgcc ggcctcggcg tcgctgacgt cgtagctggt
     3061 cacgtcggag tgccacgacc gggcctcggc cgcctcgacc ggggtgaacc tcaggccgaa
     3121 cagccgtccg gtcacctcga gcaggccgtc gtgcaccttg gcgaagtcga agtagcggcg
     3181 cacctcggtc gcgtccaccc cgtagcgttc gcgcttgatc aactcggtgt agtgccgcca
     3241 gttcgacacg tcgatcacgc tctcgccgtc ttgcccggcc cgtgcccgca gcacctcgat
     3301 ctcgcgctgc cccgacggca gcgcggcggc gctgacggtg tcgatgaact cgccgatcgc
     3361 ggcgccgctg ccgatcatct tgacctcggc gtcgtagtcg gcccagttcg cgtagccgag
     3421 caggccggcc agctcgtgcc tgagcgcgag cagctcggcc agcacg
//
LOCUS       DAOSUG010000337         3461 bp    DNA     linear   ENV 10-JUN-2024
DEFINITION  MAG TPA_asm: Micropruina sp. isolate AMR_MDS_2195
            NODE_11152_length_3461_cov_3.231944, whole genome shotgun sequence.
ACCESSION   DAOSUG010000337 DAOSUG010000000
VERSION     DAOSUG010000337.1
DBLINK      BioProject: PRJNA925477
            BioSample: SAMN32864262
            Sequence Read Archive: SRR4236673
KEYWORDS    WGS; ENV; Metagenome Assembled Genome; MAG; Third Party Data; TPA;
            TPA:assembly.
SOURCE      Micropruina sp. (wastewater metagenome)
  ORGANISM  Micropruina sp.
            Bacteria; Bacillati; Actinomycetota; Actinomycetes;
            Propionibacteriales; Nocardioidaceae; Micropruina.
REFERENCE   1  (bases 1 to 3461)
  AUTHORS   Abdulkadir,N., Saraiva,J.P., Zhang,J., Stolte,S., Gillor,O.,
            Harms,H. and Rocha,U.
  TITLE     Genome-centric analyses of 165 metagenomes show that mobile genetic
            elements are crucial for the transmission of antimicrobial
            resistance genes to pathogens in activated sludge and wastewater
  JOURNAL   Microbiol Spectr 12 (3), e0291823 (2024)
   PUBMED   38289113
REFERENCE   2  (bases 1 to 3461)
  AUTHORS   Abdulkadir,N.
  TITLE     Direct Submission
  JOURNAL   Submitted (24-MAR-2023) Environmental Microbiology, Helmholtz
            Center for Environmental Research -UFZ, Permoserstr 15, Leipzig
            04318, Germany
COMMENT     The annotation was added by the NCBI Prokaryotic Genome Annotation
            Pipeline (PGAP). Information about PGAP can be found here:
            https://www.ncbi.nlm.nih.gov/genome/annotation_prok/
            
            ##Genome-Assembly-Data-START##
            Assembly Method        :: SPAdes v. 3.11.1
            Genome Representation  :: Full
            Expected Final Version :: Yes
            Genome Coverage        :: Not Applicable
            Sequencing Technology  :: Illumina MiSeq
            ##Genome-Assembly-Data-END##
            
            ##Genome-Annotation-Data-START##
            Annotation Provider               :: NCBI
            Annotation Date                   :: 05/04/2023 04:57:10
            Annotation Pipeline               :: NCBI Prokaryotic Genome
                                                 Annotation Pipeline (PGAP)
            Annotation Method                 :: Best-placed reference protein
                                                 set; GeneMarkS-2+
            Annotation Software revision      :: 6.5
            Features Annotated                :: Gene; CDS; rRNA; tRNA; ncRNA
            Genes (total)                     :: 2,776
            CDSs (total)                      :: 2,750
            Genes (coding)                    :: 2,714
            CDSs (with protein)               :: 2,714
            Genes (RNA)                       :: 26
            tRNAs                             :: 24
            ncRNAs                            :: 2
            Pseudo Genes (total)              :: 36
            CDSs (without protein)            :: 36
            Pseudo Genes (ambiguous residues) :: 0 of 36
            Pseudo Genes (frameshifted)       :: 2 of 36
            Pseudo Genes (incomplete)         :: 32 of 36
            Pseudo Genes (internal stop)      :: 2 of 36
            ##Genome-Annotation-Data-END##
FEATURES             Location/Qualifiers
     source          1..3461
                     /organism="Micropruina sp."
                     /mol_type="genomic DNA"
                     /submitter_seqid="NODE_11152_length_3461_cov_3.231944"
                     /isolate="AMR_MDS_2195"
                     /isolation_source="Activated sludge of municipal
                     wastewater treatment plants"
                     /db_xref="taxon:2737536"
                     /environmental_sample
                     /geo_loc_name="Canada"
                     /lat_lon="49.25 N 123.1 W"
                     /collection_date="2015-06-16"
                     /metagenome_source="wastewater metagenome"
                     /note="metagenomic"
     gene            complement(556..1029)
                     /locus_tag="PLF56_10575"
     CDS             complement(556..1029)
                     /locus_tag="PLF56_10575"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_020385138.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_function: GO:0003676 - nucleic acid binding [Evidence
                     IEA];
                     GO_function: GO:0004519 - endonuclease activity [Evidence
                     IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="HNH endonuclease"
                     /protein_id="HOQ54045.1"
                     /translation="MTLVIVFNAGGEQVLHRVTLQHAISMLYRQVARVHEAVPGQHFG
                     PFPVPRSLELVRYVYTRWVYESTGHLACTLPNVLRRDGFRCAYCGRRAATRDHVVPRS
                     QGGPSTWLNLVAACASCNGRKRDRTPDQAGMPLRFAPFVPTLTDLFRPEKPPRYT"
     gene            complement(1203..1928)
                     /locus_tag="PLF56_10580"
     CDS             complement(1203..1928)
                     /locus_tag="PLF56_10580"
                     /EC_number="6.5.1.8"
                     /inference="COORDINATES: protein motif:HMM:NF013319.2"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_function: GO:0008452 - RNA ligase activity [Evidence
                     IEA];
                     GO_process: GO:0006396 - RNA processing [Evidence IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="RtcB family protein"
                     /protein_id="HOQ54046.1"
                     /translation="MYHFIEMCVADGDQRLWLTLHSGSRNIGKELAERHIATARTLEH
                     NLGLPDRDLAVFLAGTAEMDAYLHDLWWAQDYAMLSRSIMLATLRRVFADWWAGLGGG
                     QVRFDEPILCHHNYVAREVYDGVELVVTRKGAIRAAVGDRGLIPGSMGTGSYVVRGLG
                     NEVSLNSASHGAGRRMSRTRAKRTFTVDDLATQTAGIECRKDAGVIDEIPAAYKDIDA
                     VIAAQVDLVAVDARLQTLLCVKG"
     gene            1939..2727
                     /locus_tag="PLF56_10585"
     CDS             1939..2727
                     /locus_tag="PLF56_10585"
                     /inference="COORDINATES: ab initio
                     prediction:GeneMarkS-2+"
                     /note="Derived by automated computational analysis using
                     gene prediction method: GeneMarkS-2+."
                     /codon_start=1
                     /transl_table=11
                     /product="hypothetical protein"
                     /protein_id="HOQ54047.1"
                     /translation="MFEDLQSPDAAYAVGLLQTDGTHEGSVDGKGRVSVEVAARDHEV
                     LSRLAQSLGCYSSIGRRTRSTNFGDNYETATLRFYDQRTRRLLVDAGVPVGRKARTIS
                     PPTMPFAAPDYVRGLLDGDGSIGFTRKGEPFISMVTASPDIAQYLCVTIGEVCGVTRT
                     ARPNARDDVFNVMVLNQAATQLASWAWYDTEVVGIRRKKSAAVKVAAWTPPAGKEGRY
                     GVVRRAWTAEDDSVVLGQPTARAAEILGRTLSSVSVRKWRLRQV"
     gene            complement(2906..3274)
                     /locus_tag="PLF56_10590"
     CDS             complement(2906..3274)
                     /locus_tag="PLF56_10590"
                     /inference="COORDINATES: protein motif:HMM:NF013319.2"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_function: GO:0008452 - RNA ligase activity [Evidence
                     IEA];
                     GO_process: GO:0006396 - RNA processing [Evidence IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="RtcB family protein"
                     /protein_id="HOQ54048.1"
                     /translation="MTEFPVALPGATNDNTLMWAQPYDIEPAALDQLRSISRLPWVDR
                     VRVMPDVHLGKGATVGSVIAMRDAVSPAAVGVDIGCGMGAVRTNLTLLDLPDDLASLR
                     SAIERAVRVGFNGNDGTAPV"
     gene            complement(3347..3419)
                     /locus_tag="PLF56_10595"
     tRNA            complement(3347..3419)
                     /locus_tag="PLF56_10595"
                     /product="tRNA-Phe"
                     /inference="COORDINATES: profile:tRNAscan-SE:2.0.12"
                     /note="Derived by automated computational analysis using
                     gene prediction method: tRNAscan-SE."
                     /anticodon=(pos:complement(3384..3386),aa:Phe,seq:gaa)
BASE COUNT          597 a         1166 c         1131 g          567 t
ORIGIN      
        1 gagacgatcg cttcggagcg cagcaggaac cgggcgatgc tggccaggtc acgggcattc
       61 ccggccaggc tgcggactgc ctgctcggcg cggcctacca agtcgtcgag gtcggggggc
      121 agcagcagtg ggctgccaag caacaggtct ggaacgtagt gtcggtaggc cccggagcgc
      181 cggtcgcggc gaggcaggcc tgtgtcgacc gaagataccc acaccccttc atcccacgtc
      241 gccactcttc cacctgtcgt tgaccagccc tggctgatcg taatgatagc tatctaaagt
      301 ttcacatgta ggtctggtgg gtaacactaa ctgcgagcgg attggagttc caccacgggc
      361 cctagcagca gagcagggcc ccgggttggc tggcttgcgg catggcccgc caagagttcc
      421 ttgcgttgct ggacggtcac cctcgatcgt gtggcatacc gaggcgatgt gtcgacctgc
      481 gcacctcgcc ggatcgccac aggctgtgaa acccgcggaa ggagtgtggg caccagcgtg
      541 gtgcccacac tcacatcatg tgtaccgagg cggtttctcc ggccgaaaca gatccgtcag
      601 cgtcggcacg aacggtgcga agcgcagcgg catgccggcc tggtcggggg tgcggtcgcg
      661 cttgcggccg ttgcaggagg cgcaggcggc caccaggttc agccaggtgc tcgggccacc
      721 ctggctgcgc ggcaccacgt ggtcgcgggt agcggcgcgg cgtccgcagt aggcgcagcg
      781 gaacccgtcc cggcgcagca cgttgggtag cgtgcaggcc aggtgtccgg tcgactcgta
      841 gacccagcgg gtgtacacgt agcggacgag ctccagcgac cgaggcaccg gaaaggggcc
      901 gaagtgttga cccggcaccg cctcgtgcac acgcgccacc tggcgataga gcatgctgat
      961 cgcgtgctgc agcgtcaccc gatgcagcac ctgctcgccg cccgcgttga acacgatgac
     1021 gagggtcatc tccggcctcc tttccgtcag tagtgacgtc gatcagtgca aacctgttgg
     1081 acgagcgatt gacctggccg acgggtctca agctcgaccg acgaggacga gctgcccgaa
     1141 atcttggccg tacaggtctc gacaagctcg accgacgacg gccggcatcc gacgttcgga
     1201 cctcagccct tgacgcagag cagcgtctgc agtcgcgcgt ccaccgcgac caggtccacc
     1261 tgggcggcga tcacggcgtc gatgtccttg taggcggcgg ggatctcatc gatcaccccg
     1321 gcgtccttgc ggcactcgat gcccgcggtc tgggtggcca ggtcgtccac ggtgaaggtg
     1381 cgcttcgccc tggtgcgcga catgcgccgg cccgccccgt gcgaggccga gttcagcgac
     1441 acctcattgc ccaggccgcg caccacgtag ctgcccgtac ccatcgagcc cgggatcaga
     1501 ccccggtcgc cgaccgccgc gcggatggcg cccttgcggg tcaccaccaa ctcgacgccg
     1561 tcgtagacct cccgggccac gtagttgtgg tggcacagga tcggctcgtc gaacctcacc
     1621 tggccgcccc cgagcccggc ccaccagtcg gcgaacaccc ggcgcagcgt ggccagcatg
     1681 atcgaccggc tgagcatcgc gtagtcctgc gcccaccaca ggtcgtgcag gtaggcgtcc
     1741 atctcggcgg tgccggccag aaagacagcc aggtcacgat ccggcagccc gaggttgtgc
     1801 tccaacgtcc gggccgtggc gatgtgccgc tcggccaact ccttgccgat gttgcggctg
     1861 cccgagtgca gggtcagcca cagccgctgg tcgccgtcgg cgacgcacat ctcgatgaag
     1921 tggtacaatg cccccacgat gtttgaggac ctgcagtcac ccgacgctgc ttacgccgtc
     1981 ggtcttcttc agacggacgg cacccacgag ggttcagttg acggcaaagg ccgagtgtcg
     2041 gtggaagtcg ccgcgaggga ccacgaggta ctctcgcgtc tcgcgcaatc cctcgggtgc
     2101 tactcctcga tcgggcgacg cactcgaagc accaacttcg gcgacaacta cgagacggct
     2161 actctccgct tctatgacca gcgaacgcga cgactacttg tagacgcggg agtccctgtc
     2221 ggtaggaagg cccgcacaat ctctccgccg actatgccct ttgctgcgcc cgactatgtc
     2281 cgcggactcc ttgacggcga cggatccatc gggttcacca gaaagggcga acctttcatc
     2341 agcatggtca cagccagtcc ggacatcgcc cagtatctgt gtgtgacgat cggcgaggtc
     2401 tgcggtgtga caagaacggc tcgcccgaat gcgagagacg acgtgttcaa cgtcatggtc
     2461 ctcaaccagg cagccaccca gcttgcatca tgggcttggt atgacaccga agtagttggt
     2521 attcggcgca agaagtcagc tgccgtcaag gttgcagcat ggactccacc cgccgggaaa
     2581 gagggccgtt acggggttgt ccggagggcc tggaccgccg aagatgactc cgtagttctc
     2641 ggacaaccca cagcgagagc cgccgagatc cttggacgga cgctttcgag cgtgtccgtg
     2701 aggaagtggc gactcaggca agtctgaccg gtccccggcg cgggaactcg gacgtcgagc
     2761 tgctcatcgg gctgtcgttg ccgccaccca gcgaaccgca ctgcgtcaag gcacggcctt
     2821 gacgttcgtc gataccggct gcacgcagct gcccgaactg ggtgaacagc tggtcgaacc
     2881 gggcccgcag ccggtggtcg gtccgtcaca ccggtgccgt gccgtcgttg ccgttgaagc
     2941 ctacccgcac cgcccgctcg atggcggacc gcagcgacgc caggtcgtcc ggaaggtcca
     3001 acagggtcag gtttgtgcga accgcgccca tgccgcagcc gatgtcgacg cccaccgcag
     3061 caggcgagac ggcgtcgcgc atcgcgatca ccgaaccaac ggtcgcaccc ttgccgaggt
     3121 ggacgtcggg catcacccgc acccgatcca cccacggcag ccgcgagatg ctgcgcaact
     3181 ggtcgagcgc tgcgggctcg atgtcgtacg gctgcgccca catcagcgtg ttgtcgttgg
     3241 tggcgccggg cagcgccacc gggaactcgg tcatgagcac ccctttcgtt cgttcaagtg
     3301 ggggaggacg tggggcgtcc tcccccagaa cccatcccaa ggttcgtggc cagtgccggc
     3361 gtcgaaccgg cagcctcccc gtcttcggcg gggcgcttcg cctcctgagc ttcctggccg
     3421 tgccgtcgat cgccagcggg catgaaagag ccgccacact c
//
LOCUS       DAOSUG010000338         3456 bp    DNA     linear   ENV 10-JUN-2024
DEFINITION  MAG TPA_asm: Micropruina sp. isolate AMR_MDS_2195
            NODE_11171_length_3456_cov_5.186122, whole genome shotgun sequence.
ACCESSION   DAOSUG010000338 DAOSUG010000000
VERSION     DAOSUG010000338.1
DBLINK      BioProject: PRJNA925477
            BioSample: SAMN32864262
            Sequence Read Archive: SRR4236673
KEYWORDS    WGS; ENV; Metagenome Assembled Genome; MAG; Third Party Data; TPA;
            TPA:assembly.
SOURCE      Micropruina sp. (wastewater metagenome)
  ORGANISM  Micropruina sp.
            Bacteria; Bacillati; Actinomycetota; Actinomycetes;
            Propionibacteriales; Nocardioidaceae; Micropruina.
REFERENCE   1  (bases 1 to 3456)
  AUTHORS   Abdulkadir,N., Saraiva,J.P., Zhang,J., Stolte,S., Gillor,O.,
            Harms,H. and Rocha,U.
  TITLE     Genome-centric analyses of 165 metagenomes show that mobile genetic
            elements are crucial for the transmission of antimicrobial
            resistance genes to pathogens in activated sludge and wastewater
  JOURNAL   Microbiol Spectr 12 (3), e0291823 (2024)
   PUBMED   38289113
REFERENCE   2  (bases 1 to 3456)
  AUTHORS   Abdulkadir,N.
  TITLE     Direct Submission
  JOURNAL   Submitted (24-MAR-2023) Environmental Microbiology, Helmholtz
            Center for Environmental Research -UFZ, Permoserstr 15, Leipzig
            04318, Germany
COMMENT     The annotation was added by the NCBI Prokaryotic Genome Annotation
            Pipeline (PGAP). Information about PGAP can be found here:
            https://www.ncbi.nlm.nih.gov/genome/annotation_prok/
            
            ##Genome-Assembly-Data-START##
            Assembly Method        :: SPAdes v. 3.11.1
            Genome Representation  :: Full
            Expected Final Version :: Yes
            Genome Coverage        :: Not Applicable
            Sequencing Technology  :: Illumina MiSeq
            ##Genome-Assembly-Data-END##
            
            ##Genome-Annotation-Data-START##
            Annotation Provider               :: NCBI
            Annotation Date                   :: 05/04/2023 04:57:10
            Annotation Pipeline               :: NCBI Prokaryotic Genome
                                                 Annotation Pipeline (PGAP)
            Annotation Method                 :: Best-placed reference protein
                                                 set; GeneMarkS-2+
            Annotation Software revision      :: 6.5
            Features Annotated                :: Gene; CDS; rRNA; tRNA; ncRNA
            Genes (total)                     :: 2,776
            CDSs (total)                      :: 2,750
            Genes (coding)                    :: 2,714
            CDSs (with protein)               :: 2,714
            Genes (RNA)                       :: 26
            tRNAs                             :: 24
            ncRNAs                            :: 2
            Pseudo Genes (total)              :: 36
            CDSs (without protein)            :: 36
            Pseudo Genes (ambiguous residues) :: 0 of 36
            Pseudo Genes (frameshifted)       :: 2 of 36
            Pseudo Genes (incomplete)         :: 32 of 36
            Pseudo Genes (internal stop)      :: 2 of 36
            ##Genome-Annotation-Data-END##
FEATURES             Location/Qualifiers
     source          1..3456
                     /organism="Micropruina sp."
                     /mol_type="genomic DNA"
                     /submitter_seqid="NODE_11171_length_3456_cov_5.186122"
                     /isolate="AMR_MDS_2195"
                     /isolation_source="Activated sludge of municipal
                     wastewater treatment plants"
                     /db_xref="taxon:2737536"
                     /environmental_sample
                     /geo_loc_name="Canada"
                     /lat_lon="49.25 N 123.1 W"
                     /collection_date="2015-06-16"
                     /metagenome_source="wastewater metagenome"
                     /note="metagenomic"
     gene            complement(<1..412)
                     /locus_tag="PLF56_10600"
     CDS             complement(<1..412)
                     /locus_tag="PLF56_10600"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_003866496.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="DNA-directed RNA polymerase subunit beta"
                     /protein_id="HOQ54049.1"
                     /translation="MAVSRSASKNTNVVSASGRISFAKIAEPLEVPNLLDLQIESFDW
                     LIGNETWRTRVATALAEGRTDVNTKSGLEEIFEEISPIEDLSQTMSLSFRDNRFEDPK
                     HSVEECKDRDVTYAAPLFVTAEFMNNDTGEIKSQT"
     gene            complement(1423..>1765)
                     /locus_tag="PLF56_10605"
     CDS             complement(1423..>1765)
                     /locus_tag="PLF56_10605"
                     /inference="COORDINATES: ab initio
                     prediction:GeneMarkS-2+"
                     /note="Derived by automated computational analysis using
                     gene prediction method: GeneMarkS-2+."
                     /codon_start=2
                     /transl_table=11
                     /product="hypothetical protein"
                     /protein_id="HOQ54050.1"
                     /translation="LIGNAFDMFELRQAEAKLTLLEGRPDEAREQVKALIADPVMRPA
                     VVSNGRAEELLAEIELDLGQHQPAREAFIRAAGLYGVQEQYRQANQCWQRAADLHPLY
                     VHHVDSVASQA"
     assembly_gap    1766..1865
                     /estimated_length=100
                     /gap_type="within scaffold"
                     /linkage_evidence="paired-ends"
     gene            complement(<1866..2329)
                     /locus_tag="PLF56_10610"
     CDS             complement(<1866..2329)
                     /locus_tag="PLF56_10610"
                     /inference="COORDINATES: protein motif:HMM:NF024252.2"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_function: GO:0003677 - DNA binding [Evidence IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="helix-turn-helix transcriptional regulator"
                     /protein_id="HOQ54051.1"
                     /translation="MTASLAHNGFGARLRQTRKRLGMSQTEVGGERFSGSYISHLESG
                     RRSASADVIEFLAMRMGVTAEELGMEPALPLSTFDNLDSTEALEHLLVAERAWYDRDW
                     ATAGELATRAAASALQSGQPARHWQALYVQTQSALNDGNHADAIHFAEQLAS"
     gene            2438..2584
                     /locus_tag="PLF56_10615"
     CDS             2438..2584
                     /locus_tag="PLF56_10615"
                     /inference="COORDINATES: ab initio
                     prediction:GeneMarkS-2+"
                     /note="Derived by automated computational analysis using
                     gene prediction method: GeneMarkS-2+."
                     /codon_start=1
                     /transl_table=11
                     /product="hypothetical protein"
                     /protein_id="HOQ54052.1"
                     /translation="MNKTFRKAFVAAAALIAIVVVPVTAAAQDHAGGYGDWPASRTIK
                     THTK"
     gene            complement(3217..>3456)
                     /gene="rplL"
                     /locus_tag="PLF56_10620"
     CDS             complement(3217..>3456)
                     /gene="rplL"
                     /locus_tag="PLF56_10620"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_014167065.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_component: GO:0000315 - organellar large ribosomal
                     subunit [Evidence IEA];
                     GO_component: GO:0022625 - cytosolic large ribosomal
                     subunit [Evidence IEA];
                     GO_function: GO:0003735 - structural constituent of
                     ribosome [Evidence IEA];
                     GO_process: GO:0006412 - translation [Evidence IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="50S ribosomal protein L7/L12"
                     /protein_id="HOQ54053.1"
                     /translation="GDAAEEEEVSNEVDVILESAGDKKIQVIKEVRALTSLGLKEAKD
                     LVEAAPKPVLEKVTREAAAKAKEALEAAGAAVSVK"
BASE COUNT          578 a         1107 c         1090 g          581 t
ORIGIN      
        1 ccgtctggct cttgatctcg ccggtgtcgt tgttcatgaa ctcggcggtg acgaacagcg
       61 gtgcggcgta ggtgacgtcg cggtccttgc actcctccac cgagtgcttg gggtcctcga
      121 accggttgtc gcggaacgac agcgacatgg tctgggacag atcctcgatc ggagagatct
      181 cttcgaagat ctcttccaga ccggacttcg tgttgacgtc cgtgcggcct tcggccaagg
      241 cggtggccac gcgggttcgc caggtttcgt tgccgatcag ccagtcgaac gactcgatct
      301 gcagatcgag caggttcgga acctcgagag gttcggcgat cttggcgaac gagatccggc
      361 ctgaagcgga gacgacgttg gtgttcttcg acgcagagcg cgagacggcc aaggtgatgg
      421 tccttccaag aaacccggcg ctgagccggt tctttgcacg cagaacggcc cgcgtcaaat
      481 cacgggtcgt tggtgagcga tgggcaagga tcaactgtag tgcggttgcc gccgtttgcc
      541 aaatcggcag gactcatcag cgccggcgac agatgcacct caacaaggag gcccggccca
      601 cgctggacgg tgtcccgact gtactgcggt ggacggcttc gcgcacagca atcacgcact
      661 acgccggtgg gcggcctgtc tgtccactgg cctggacgtt gcgggcacgc acgcggggca
      721 cccgttgagc gccagagtca tctgcgcctg aggcttcggc ttcggcacct gtctcgaagg
      781 cacgtgatga tcctcgctcg gtcgggctaa acctggatcc accgatgggc gccgtcgcga
      841 gccgcaccag acgtgtgggc tggcaaaccg tccgcacgaa ggcataccgg gtggcctgcc
      901 ccgcgtagcc ggcgacgcca gggccccgaa tggcgccgcg cgcccgccta accatctctg
      961 gcccgcgaag ctacgcgggc cagagatggt tagggaggca ttgcgtgggg cggttgcagt
     1021 gggcgcagat cggtggatcc gggctgaacg gcgagacctt cgggtgcggg ttttcgacac
     1081 gctggacgag cgtcgagacc ctcacgggta aaaactgcga ccacgagccg gacccgcctt
     1141 gatacgcatc tgcctcgagc ttgccttggg gtgggcgacc ccacctggcc gcgagcgtgc
     1201 acctagcttg acagcgatgc gcttacccgg aaaacggagg agccgccgtc atggacggcg
     1261 gctcagactc ctggggcggg ggaccccttc gcactaatcg gtgctcgcct cgggcaacgg
     1321 gaggaaactc acaaggcgag caccgatctc agtgcccatc aacgacaata cggggggttg
     1381 tgccgtcgac atgactaact agaccatcac ttgacaaggc tgtcaagcct gggatgcgac
     1441 ggagtccaca tggtggacgt acaggggatg gaggtcggcc gctcgctgcc agcactggtt
     1501 cgcctgccgg tactgctcct gcacgccgta cagcccggcg gcgcggatga acgcctcacg
     1561 cgcgggctgg tgctggccca gatcgagttc gatctcagcc agcaactcct ccgctcggcc
     1621 gttgctgacc accgcagggc gcatgactgg atcggcgatc agtgccttga cctgttcgcg
     1681 agcctcgtcg ggtcggcctt cgagcagggt gagcttggcc tcggcttggc gaagctcgaa
     1741 catgtcgaag gcattaccga tcagcnnnnn nnnnnnnnnn nnnnnnnnnn nnnnnnnnnn
     1801 nnnnnnnnnn nnnnnnnnnn nnnnnnnnnn nnnnnnnnnn nnnnnnnnnn nnnnnnnnnn
     1861 nnnnntgcga ggcaagctgc tcggcgaaat ggatcgcatc ggcatgattg ccatcgttca
     1921 acgcggattg cgtctgcacg tatagggcct gccaatggcg cgccggctgc ccactctgca
     1981 gcgccgacgc cgccgcgcga gtggccaact cgcccgcggt tgcccagtca cggtcgtacc
     2041 aggcccgttc cgccaccagt agatgttcga gagcctcggt gctgtcaagg ttgtcaaatg
     2101 ttgacagcgg cagcgcgggc tccatgccca actcttcggc cgtcacgccc atgcgcatgg
     2161 caagaaactc gatcacgtcg gccgaagccg atcgacgccc gctttcgagg tgggagatat
     2221 aactgccgct gaagcgttcg ccgcccacct ccgtctgcga cataccgaga cgtttgcgag
     2281 tctgacgcag gcgcgcgccg aacccgttgt gggcaagcga agccgtcaag gcgcacctcc
     2341 tggggagcaa tcaacccaat ctgaccacta agttgacact ctcgtcaagt ctggatagcg
     2401 tttgtcaaca gcaccaaacc acttttccgg gggaagaatg aacaagacgt tccgcaaggc
     2461 cttcgtggcc gcggcagccc taatcgccat cgtggtcgtt cccgtgaccg cagcagcaca
     2521 ggaccacgcc ggcggttacg gcgactggcc agccagccga accatcaaga cccacaccaa
     2581 gtagcacgac ccaaggttca gcccttccca cccccgcaga ccgatcggcg gcggaacttg
     2641 cgttccgtcg ccgattagtg ctgccaacgc aagtgcggta gcacccagcg gccttttgtc
     2701 ggcggcggaa cctgcgaccc gtcgccggtt tgccccacct tcggcgggcg cacagcaatc
     2761 ttcgacaccg ggtcggtggt cgtcgaggcc accggcaccg gcggaccagc acgcgtccac
     2821 gacgcgcggg cgaggtgcgc ccctccaacc ccgacgcagc agccgaaccg cgggcccctt
     2881 caacgatgcg gctctccgag cctcccggcc gtcagccgtg gctgaagagg ggctggtggg
     2941 cctcggactg gcgcgagccg gtcacccgtg tccacacagt gacgtacgtg ggagcgaccc
     3001 gagcggctat acaccgtcca cctgccgacg ctggcgctgg cggtcgcggg cggcgtggca
     3061 caggcgtcgc tgaccgttga gtcttcccgg gcctggcgtc agccggccgc agtcgccacg
     3121 cctgagacgt ccgctcgtcg tgacaggtct gcatggacgc aacagcggcg gagcccacgg
     3181 ggctccgccg ctgttggtgt aaccggtttg gtcagttcac ttgacgctga cggcggcacc
     3241 ggcggcctcg agggcctcct tggccttcgc cgcagcctcg cgggtgacct tctccaggac
     3301 gggcttggga gcggcctcga ccagatcctt ggcctccttc aggcccaggc tggtgagcgc
     3361 acgcacctcc ttgatgacct ggatcttctt gtcgccagcg gactcgagga tcacgtcgac
     3421 ctcgttgctg acctcttctt cttcggcagc gtcgcc
//
LOCUS       DAOSUG010000339         3449 bp    DNA     linear   ENV 10-JUN-2024
DEFINITION  MAG TPA_asm: Micropruina sp. isolate AMR_MDS_2195
            NODE_11205_length_3449_cov_3.957867, whole genome shotgun sequence.
ACCESSION   DAOSUG010000339 DAOSUG010000000
VERSION     DAOSUG010000339.1
DBLINK      BioProject: PRJNA925477
            BioSample: SAMN32864262
            Sequence Read Archive: SRR4236673
KEYWORDS    WGS; ENV; Metagenome Assembled Genome; MAG; Third Party Data; TPA;
            TPA:assembly.
SOURCE      Micropruina sp. (wastewater metagenome)
  ORGANISM  Micropruina sp.
            Bacteria; Bacillati; Actinomycetota; Actinomycetes;
            Propionibacteriales; Nocardioidaceae; Micropruina.
REFERENCE   1  (bases 1 to 3449)
  AUTHORS   Abdulkadir,N., Saraiva,J.P., Zhang,J., Stolte,S., Gillor,O.,
            Harms,H. and Rocha,U.
  TITLE     Genome-centric analyses of 165 metagenomes show that mobile genetic
            elements are crucial for the transmission of antimicrobial
            resistance genes to pathogens in activated sludge and wastewater
  JOURNAL   Microbiol Spectr 12 (3), e0291823 (2024)
   PUBMED   38289113
REFERENCE   2  (bases 1 to 3449)
  AUTHORS   Abdulkadir,N.
  TITLE     Direct Submission
  JOURNAL   Submitted (24-MAR-2023) Environmental Microbiology, Helmholtz
            Center for Environmental Research -UFZ, Permoserstr 15, Leipzig
            04318, Germany
COMMENT     The annotation was added by the NCBI Prokaryotic Genome Annotation
            Pipeline (PGAP). Information about PGAP can be found here:
            https://www.ncbi.nlm.nih.gov/genome/annotation_prok/
            
            ##Genome-Assembly-Data-START##
            Assembly Method        :: SPAdes v. 3.11.1
            Genome Representation  :: Full
            Expected Final Version :: Yes
            Genome Coverage        :: Not Applicable
            Sequencing Technology  :: Illumina MiSeq
            ##Genome-Assembly-Data-END##
            
            ##Genome-Annotation-Data-START##
            Annotation Provider               :: NCBI
            Annotation Date                   :: 05/04/2023 04:57:10
            Annotation Pipeline               :: NCBI Prokaryotic Genome
                                                 Annotation Pipeline (PGAP)
            Annotation Method                 :: Best-placed reference protein
                                                 set; GeneMarkS-2+
            Annotation Software revision      :: 6.5
            Features Annotated                :: Gene; CDS; rRNA; tRNA; ncRNA
            Genes (total)                     :: 2,776
            CDSs (total)                      :: 2,750
            Genes (coding)                    :: 2,714
            CDSs (with protein)               :: 2,714
            Genes (RNA)                       :: 26
            tRNAs                             :: 24
            ncRNAs                            :: 2
            Pseudo Genes (total)              :: 36
            CDSs (without protein)            :: 36
            Pseudo Genes (ambiguous residues) :: 0 of 36
            Pseudo Genes (frameshifted)       :: 2 of 36
            Pseudo Genes (incomplete)         :: 32 of 36
            Pseudo Genes (internal stop)      :: 2 of 36
            ##Genome-Annotation-Data-END##
FEATURES             Location/Qualifiers
     source          1..3449
                     /organism="Micropruina sp."
                     /mol_type="genomic DNA"
                     /submitter_seqid="NODE_11205_length_3449_cov_3.957867"
                     /isolate="AMR_MDS_2195"
                     /isolation_source="Activated sludge of municipal
                     wastewater treatment plants"
                     /db_xref="taxon:2737536"
                     /environmental_sample
                     /geo_loc_name="Canada"
                     /lat_lon="49.25 N 123.1 W"
                     /collection_date="2015-06-16"
                     /metagenome_source="wastewater metagenome"
                     /note="metagenomic"
     gene            complement(101..763)
                     /locus_tag="PLF56_10625"
     CDS             complement(101..763)
                     /locus_tag="PLF56_10625"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_014845342.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_function: GO:0003677 - DNA binding [Evidence IEA];
                     GO_process: GO:0000160 - phosphorelay signal transduction
                     system [Evidence IEA];
                     GO_process: GO:0006355 - regulation of DNA-templated
                     transcription [Evidence IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="response regulator transcription factor"
                     /protein_id="HOQ54054.1"
                     /translation="MSRILIIEDEPRIAAFVAKGLAAAGYTTHVESSGVIGAQLAGSG
                     DVDLVILDVGLPDIDGFEVLNRLRGQGVGVPVIMLTARSSVTDTVAGLEGGADDYMGK
                     PFSFEELLARVRLRLRPDAAATATATTLTHRDLTLDLRTRRANVGGREVDLSAREFTL
                     AETFLMNPGQVLSREQLLSGVWGYDFDPASNVVDVYVRYLRRKLGAERFETVRGMGYR
                     LV"
     gene            complement(760..2247)
                     /locus_tag="PLF56_10630"
     CDS             complement(760..2247)
                     /locus_tag="PLF56_10630"
                     /inference="COORDINATES: protein motif:HMM:NF014567.2"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_function: GO:0000156 - phosphorelay response regulator
                     activity [Evidence IEA];
                     GO_function: GO:0004673 - protein histidine kinase
                     activity [Evidence IEA];
                     GO_function: GO:0005524 - ATP binding [Evidence IEA];
                     GO_process: GO:0007165 - signal transduction [Evidence
                     IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="HAMP domain-containing sensor histidine kinase"
                     /protein_id="HOQ54055.1"
                     /translation="MASATQTPSSSPPRRSWAGLSTVRSRVIAAVIGLTALALTIAGL
                     TAYSLERALAEHRVQGYLDRSVEEFRVLAQQGLDSTGRPFSESSVDELLRTAMQRHVL
                     AANESHLGIRGGTLAWTAAEGVPLRPEQDAEFLAAVLPLSTLDHTTRSRLSTSRHDYF
                     FAVIPVHFPSENGALVHVFDMRAEDRSLQGTYGTYALVALGSLVVVGVLIWLLVGRLL
                     RPIRLLRTTAEDITEHDLSRRIPVHGNDDLSALTGTVNAMLDRLQGVLDSQRQLLDDV
                     GHELRTPVTIVRGHLELMRTDDPADVQGTRTLALDELDRMARLVDDLLTLAKSESADF
                     VQPCATDIGRLTDETLEKARALAPRRWQLDAMADVEAVLDPQRITQAWLQLASNAVKY
                     SPADSPIAMGSALRDGRLQLWVRDRGIGIAPAERERVLQRFVRGEAAPLRADGTGLGL
                     AIVDRIVRAHGGTVQIDSSAAEGSTITLVLPQRAITPSQPPMEAT"
     gene            complement(2395..2468)
                     /locus_tag="PLF56_10635"
     tRNA            complement(2395..2468)
                     /locus_tag="PLF56_10635"
                     /product="tRNA-Phe"
                     /inference="COORDINATES: profile:tRNAscan-SE:2.0.12"
                     /note="Derived by automated computational analysis using
                     gene prediction method: tRNAscan-SE."
                     /anticodon=(pos:complement(2432..2434),aa:Phe,seq:gaa)
     gene            complement(2491..2564)
                     /locus_tag="PLF56_10640"
     tRNA            complement(2491..2564)
                     /locus_tag="PLF56_10640"
                     /product="tRNA-Asp"
                     /inference="COORDINATES: profile:tRNAscan-SE:2.0.12"
                     /note="Derived by automated computational analysis using
                     gene prediction method: tRNAscan-SE."
                     /anticodon=(pos:complement(2528..2530),aa:Asp,seq:gtc)
     gene            complement(2750..3304)
                     /locus_tag="PLF56_10645"
     CDS             complement(2750..3304)
                     /locus_tag="PLF56_10645"
                     /inference="COORDINATES: ab initio
                     prediction:GeneMarkS-2+"
                     /note="Derived by automated computational analysis using
                     gene prediction method: GeneMarkS-2+."
                     /codon_start=1
                     /transl_table=11
                     /product="hypothetical protein"
                     /protein_id="HOQ54056.1"
                     /translation="MTTTRTWVVVLSGRHAQQAVDTQRIATGTGKRAVLARMSTGDHI
                     VVYSPRTDHPGGAPLGAVTVVGTITGEQPEGDEASGFGLAAELRRVDPVPLDRLRDHL
                     PVPLLRFGSLALSEAQGLAVWGVVAGDRPEYAMANASPAALGPGQQPEPSPDPAPLTS
                     TGPAQPSSVSVNAATHPADAGVVR"
BASE COUNT          553 a         1157 c         1217 g          522 t
ORIGIN      
        1 ctacgccgag gcacgtccct ggtggcgggg gacacacctg agacgggagg gcggcagtga
       61 gctacgccga ggcacgtccc tggtggcggg ggacacacca ctagaccagc cggtacccca
      121 tgccccggac ggtctcgaac cgttccgcgc ccagcttgcg ccgcaggtag cgcacgtaca
      181 cgtcgaccac gttcgatgcg ggatcgaagt cgtagcccca cacgcccgac agcagttgct
      241 cacggctgag cacctggccc gggttcatca agaacgtctc ggccagggtg aactcgcggg
      301 ccgacaggtc cacctcgcgt ccgcccacat tcgctcggcg ggtgcgcagg tcgagcgtga
      361 ggtcgcggtg cgtcagtgtc gtggcggtgg cggtggcggc agcgtccgga cgcagccgca
      421 gccgcacccg tgcgagcagc tcttcgaaac tgaacggctt gcccatgtag tcgtcggcgc
      481 caccctccag tccggcgacg gtgtccgtca cggacgagcg tgcggtgagc atgatcaccg
      541 gtacgcccac accctggcca cgcagccggt tcagcacctc gaagccgtcg atgtcgggca
      601 gcccgacgtc gagaatcacc aggtccacgt cacccgaacc ggcgagctgc gcgccgatca
      661 caccgctgct ctcgacgtgc gtggtgtagc cggcggctgc gagtcctttc gccacgaacg
      721 cggcaatgcg gggttcgtcc tcgatgatca ggatgcggct cacgtggcct ccattggtgg
      781 ctgagaaggg gtgatcgccc gctgcggcag caccagagtg atcgtgctgc cctccgcggc
      841 ggacgagtcg atctgcaccg tgccgccatg cgcccggacg atgcggtcga cgatcgccag
      901 gccgaggccc gtgccgtccg cgcgaagggg ggcggcctcg ccgcggacga accgctgcag
      961 cacacgctcg cgttcggcgg gggcgatacc gatgccccga tcgcgcaccc acagctgcaa
     1021 ccggccatcg cgcagcgcgc tgcccattgc gatcgggctg tcggcggggg agtacttgac
     1081 cgcgttggac gccagctgca gccacgcctg ggtgattcgt tgtgggtcga gcaccgcttc
     1141 gacgtcggcc attgcatcca gttgccagcg ccggggtgcc agcgcacgag ccttctcgag
     1201 cgtctcgtcc gtgagccgcc cgatgtcggt tgcgcagggc tgcacgaagt cggcactctc
     1261 ggatttggcc agggtcaaca ggtcgtcgac caggcgggcc atccggtcga gttcgtccag
     1321 ggccagggtg cgggtgcctt gcacgtcagc ggggtcgtcg gtgcgcatca gctcgaggtg
     1381 gccgcgcacg atggtgacgg gggtgcgcag ttcgtggccg acgtcgtcga gcagctgacg
     1441 ctgactgtcg agaacaccct gcagccggtc gagcatggcg ttcacggtac cggtcagggc
     1501 cgacaggtcg tcgttgccgt gcaccggtat gcgcctgctc aggtcgtgct cggtgatgtc
     1561 ttcggcggtg gtgcgcagca accggatcgg gcgcagcagc cgtccgacga gcagccagat
     1621 cagaacgccg acgacgacca gtgaccccag agcgaccagg gcataggtgc cgtaggttcc
     1681 ctggagcgag cggtcttcgg cacgcatgtc gaacacatgc accaacgcgc cgttctcgct
     1741 cggaaagtgc accggaatga cggcgaagaa gtagtcgtgg cgactggtgc tgagccggct
     1801 gcgggtggtg tggtccaggg tgctcagcgg aaggacggcg gcgaggaact cggcgtcctg
     1861 ctcgggtcgc agcggtacac cttcggccgc cgtccaggcc agcgtgccgc cccggatgcc
     1921 gagatggctt tcgttggccg ccagcacgtg gcgctgcatg gcggtgcgca gcagctcgtc
     1981 gacgctcgac tcgctgaacg gacgcccggt ggagtcgagc ccctgctgag ccagtacccg
     2041 gaactcctcg accgagcggt cgaggtagcc ctgcacccgg tgttcggcca aggcgcgttc
     2101 gagcgaatag gcggtcaaac cggcgatggt gagggcgagt gcggtgagcc cgatcacggc
     2161 ggcgatcacc cggctgcgga cggtgctcaa gccggcccac gaccggcgcg gcggcgacga
     2221 cgacggagtc tgcgtcgcac tggccatggg cacagtgtgc caaggcgggt cgaggggccg
     2281 gtgaggccaa ggtgagaacc ttctcaccac cggcgggccc cacacgctct gggttgacgc
     2341 agccagctgc ccaccgacaa gcgaaaacgc cccgagaatc cggggcgtct gcggtggcca
     2401 ggaccggggt cgaaccggtg acctttcact tttcaggcga acgctactac cagctgagct
     2461 acctggccga tgagcgatgc ggtcgctcag cgaccccgac gggactcgaa cccgcgacct
     2521 ccgccgtgac agggcggcgc gctaaccaac tgcgctacgg ggccttgaca ggcctcaccg
     2581 gtggtttccc accggccacg aaactataac agcccgccgc ggtggctagg taatcgaccg
     2641 gcccgacccg atgccaggtg cctgctgcgc gtcgttgacc gatgcgcggt gcccgcgtgg
     2701 cgactgcggt tgactcacgc tgaccaccgg cggggcgggg gtcgttgact catctgacga
     2761 cgcccgcgtc cgccggatgc gtggctgcgt tgacggagac ggaggacggc tgtgccgggc
     2821 cggtcgaggt gaggggagcc ggatcgggag acggttccgg ttgctgcccg gggccgagtg
     2881 cagcggggga tgcgttcgcc attgcgtact caggccgatc gcctgcgacc acgccccaga
     2941 ccgccaggcc ctgcgcctcg ctcaatgcga gagaaccgaa tcgcaacagc ggcacgggca
     3001 ggtgatcgcg caaccgatca agcggtacgg ggtcgactcg cctcaactcg gcggcgagcc
     3061 cgaaaccgga ggcctcatcg ccctcgggtt gctcgccggt gatcgtgccg accaccgtca
     3121 ccgcacccag cggggcgcca cccgggtggt cggtgcgagg ggagtagacc acgatgtggt
     3181 cacccgtgga catgcgcgcc agcacggctc gcttaccggt gccggtggcg atgcgctggg
     3241 tatcaacggc ctgctgtgca tgccggccgg aaagcaccac cacccaggtt cgtgtcgtag
     3301 tcacgcccgg attatctgcc cgagatcgag aaagtgttca gaatcgcccc ccacggcgcc
     3361 gcagtgtgag cctccgatct ccgtgcgaca cagtcgcctc caggccgtcc acgcgccgag
     3421 ctgtgaagcg ttcccatgct ctgtcctga
//
LOCUS       DAOSUG010000340         3439 bp    DNA     linear   ENV 10-JUN-2024
DEFINITION  MAG TPA_asm: Micropruina sp. isolate AMR_MDS_2195
            NODE_11237_length_3439_cov_4.139775, whole genome shotgun sequence.
ACCESSION   DAOSUG010000340 DAOSUG010000000
VERSION     DAOSUG010000340.1
DBLINK      BioProject: PRJNA925477
            BioSample: SAMN32864262
            Sequence Read Archive: SRR4236673
KEYWORDS    WGS; ENV; Metagenome Assembled Genome; MAG; Third Party Data; TPA;
            TPA:assembly.
SOURCE      Micropruina sp. (wastewater metagenome)
  ORGANISM  Micropruina sp.
            Bacteria; Bacillati; Actinomycetota; Actinomycetes;
            Propionibacteriales; Nocardioidaceae; Micropruina.
REFERENCE   1  (bases 1 to 3439)
  AUTHORS   Abdulkadir,N., Saraiva,J.P., Zhang,J., Stolte,S., Gillor,O.,
            Harms,H. and Rocha,U.
  TITLE     Genome-centric analyses of 165 metagenomes show that mobile genetic
            elements are crucial for the transmission of antimicrobial
            resistance genes to pathogens in activated sludge and wastewater
  JOURNAL   Microbiol Spectr 12 (3), e0291823 (2024)
   PUBMED   38289113
REFERENCE   2  (bases 1 to 3439)
  AUTHORS   Abdulkadir,N.
  TITLE     Direct Submission
  JOURNAL   Submitted (24-MAR-2023) Environmental Microbiology, Helmholtz
            Center for Environmental Research -UFZ, Permoserstr 15, Leipzig
            04318, Germany
COMMENT     The annotation was added by the NCBI Prokaryotic Genome Annotation
            Pipeline (PGAP). Information about PGAP can be found here:
            https://www.ncbi.nlm.nih.gov/genome/annotation_prok/
            
            ##Genome-Assembly-Data-START##
            Assembly Method        :: SPAdes v. 3.11.1
            Genome Representation  :: Full
            Expected Final Version :: Yes
            Genome Coverage        :: Not Applicable
            Sequencing Technology  :: Illumina MiSeq
            ##Genome-Assembly-Data-END##
            
            ##Genome-Annotation-Data-START##
            Annotation Provider               :: NCBI
            Annotation Date                   :: 05/04/2023 04:57:10
            Annotation Pipeline               :: NCBI Prokaryotic Genome
                                                 Annotation Pipeline (PGAP)
            Annotation Method                 :: Best-placed reference protein
                                                 set; GeneMarkS-2+
            Annotation Software revision      :: 6.5
            Features Annotated                :: Gene; CDS; rRNA; tRNA; ncRNA
            Genes (total)                     :: 2,776
            CDSs (total)                      :: 2,750
            Genes (coding)                    :: 2,714
            CDSs (with protein)               :: 2,714
            Genes (RNA)                       :: 26
            tRNAs                             :: 24
            ncRNAs                            :: 2
            Pseudo Genes (total)              :: 36
            CDSs (without protein)            :: 36
            Pseudo Genes (ambiguous residues) :: 0 of 36
            Pseudo Genes (frameshifted)       :: 2 of 36
            Pseudo Genes (incomplete)         :: 32 of 36
            Pseudo Genes (internal stop)      :: 2 of 36
            ##Genome-Annotation-Data-END##
FEATURES             Location/Qualifiers
     source          1..3439
                     /organism="Micropruina sp."
                     /mol_type="genomic DNA"
                     /submitter_seqid="NODE_11237_length_3439_cov_4.139775"
                     /isolate="AMR_MDS_2195"
                     /isolation_source="Activated sludge of municipal
                     wastewater treatment plants"
                     /db_xref="taxon:2737536"
                     /environmental_sample
                     /geo_loc_name="Canada"
                     /lat_lon="49.25 N 123.1 W"
                     /collection_date="2015-06-16"
                     /metagenome_source="wastewater metagenome"
                     /note="metagenomic"
     gene            <1..407
                     /locus_tag="PLF56_10650"
     CDS             <1..407
                     /locus_tag="PLF56_10650"
                     /inference="COORDINATES: ab initio
                     prediction:GeneMarkS-2+"
                     /note="Derived by automated computational analysis using
                     gene prediction method: GeneMarkS-2+."
                     /codon_start=3
                     /transl_table=11
                     /product="hypothetical protein"
                     /protein_id="HOQ54057.1"
                     /translation="YGDNNGVIGAYDAIITTNLNGRQARWTLTLIRDYGPKLNFADGG
                     APTQIVCWDWWGAVWITCGNHYFTNLPNALSTRWTSKMIYGNRLENADTYHGAFETYF
                     KAAGYSRRWVASTLNTNDWRCPRGNGDCKMVG"
     gene            404..796
                     /locus_tag="PLF56_10655"
     CDS             404..796
                     /locus_tag="PLF56_10655"
                     /inference="COORDINATES: protein motif:HMM:NF039605.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="DUF5956 family protein"
                     /protein_id="HOQ54058.1"
                     /translation="MSWDENPVLSGAGLYPLPDNGYGALIAWLGGPERTLRAVDLARG
                     EFVVKEWVDGVVTERQITDAELAEADDLVDEYLAEAGIPPRPRGYLWFTTVDLGHLDR
                     ELSRRIPGNTVVLPRGMAEHIRAVIESV"
     gene            complement(857..1159)
                     /locus_tag="PLF56_10660"
     CDS             complement(857..1159)
                     /locus_tag="PLF56_10660"
                     /inference="COORDINATES: ab initio
                     prediction:GeneMarkS-2+"
                     /note="Derived by automated computational analysis using
                     gene prediction method: GeneMarkS-2+."
                     /codon_start=1
                     /transl_table=11
                     /product="hypothetical protein"
                     /protein_id="HOQ54059.1"
                     /translation="MANEANDALGWDLGAEGSVPGPWGNRMGDLIEDHNKRLAVLIDP
                     QTALSAVKADNGKCQAFDTRAIWEGVAQCGYILTKELGDPGFWCPGSPSRPSEADE"
     gene            1381..1536
                     /locus_tag="PLF56_10665"
     CDS             1381..1536
                     /locus_tag="PLF56_10665"
                     /inference="COORDINATES: protein motif:HMM:NF024139.2"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_function: GO:0003677 - DNA binding [Evidence IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="helix-turn-helix domain-containing protein"
                     /protein_id="HOQ54060.1"
                     /translation="MAQAAERTGISRKTLRRRIASGQLPVSSSGRRILRVRPEAVDAM
                     LRPFWLS"
     gene            complement(1564..2652)
                     /locus_tag="PLF56_10670"
     CDS             complement(1564..2652)
                     /locus_tag="PLF56_10670"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_013863438.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_function: GO:0004803 - transposase activity [Evidence
                     IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="IS630 family transposase"
                     /protein_id="HOQ54061.1"
                     /translation="MPSPAAAPLRVSPVQREVLEKVARSATAAHREVVRARVLLDSAD
                     GCANLAIAQWHGVTAVTVRSWRAAFEADGLANWGKVAKGRGRKASVSEEQVAEIVELT
                     TKTKPAGATHWSTRTMAERAGVSKDTVRRIWNELGLQPHRVDTFKLSNDPAFTEKLID
                     VVGLYLDPPEKAIVLCMDEKSQIQALDRTQASLPMVPGRAGTMTHDYKRNGTTTLFAA
                     LDVLTGTVIGQCLPRHRHEEFLAFLKTIDREVPQHLQVHLILDNYATHKHESVERWLK
                     RHKRFHLHFTPTSSSWLNQVERWFRDLTEKNLRRGVFASVPDLIDSIQEYLDASNDNP
                     KPYTWTATAESILAKVTRARAALDQAVS"
     gene            2729..2980
                     /locus_tag="PLF56_10675"
     CDS             2729..2980
                     /locus_tag="PLF56_10675"
                     /inference="COORDINATES: ab initio
                     prediction:GeneMarkS-2+"
                     /note="Derived by automated computational analysis using
                     gene prediction method: GeneMarkS-2+."
                     /codon_start=1
                     /transl_table=11
                     /product="hypothetical protein"
                     /protein_id="HOQ54062.1"
                     /translation="MTDVATIKVPRVVRDRVRDAARAAHLTQAQLIEEMLEERRKAEF
                     WAALEAETPDQEYLDDLAEADAAFAGDAEESIARFEAGE"
     gene            2977..3315
                     /locus_tag="PLF56_10680"
     CDS             2977..3315
                     /locus_tag="PLF56_10680"
                     /EC_number="3.1.-.-"
                     /inference="COORDINATES: protein motif:HMM:NF014505.2"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_function: GO:0003677 - DNA binding [Evidence IEA];
                     GO_function: GO:0003723 - RNA binding [Evidence IEA];
                     GO_function: GO:0004521 - endoribonuclease activity
                     [Evidence IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="type II toxin-antitoxin system PemK/MazF family
                     toxin"
                     /protein_id="HOQ54063.1"
                     /translation="MIQQIARGDVVWASMDPTIGREQAKHRPWLVLSEPALHRARGLL
                     IAVPLTHTDRGWSTHVKLTSRDSATTVAMCEQVKSMSIDRVTRVDRAPYPLGQVDQVH
                     QILALLTGGR"
BASE COUNT          603 a         1108 c         1120 g          608 t
ORIGIN      
        1 cgtacggtga caacaacggc gtaatcggcg cctacgacgc catcatcaca accaacttga
       61 acggacgcca ggctcgatgg acgctaacac tgattcgtga ttacggaccc aagttgaact
      121 ttgccgacgg aggcgccccc acgcaaatcg tgtgctggga ctggtggggc gcagtgtgga
      181 tcacctgtgg taatcactac ttcacaaatc tgcccaatgc tttgagcact cggtggacga
      241 gcaaaatgat ctacggaaac cggttggaga acgccgacac atatcacggt gcattcgaga
      301 catatttcaa ggctgccggc tactcgcgcc gctgggtagc gagcaccctc aacaccaacg
      361 attggcgttg ccccagaggc aacggcgact gcaaaatggt cggatgagtt gggacgagaa
      421 ccctgtcctg tcgggagctg ggctctaccc cttgcccgac aacggctatg gtgccctgat
      481 cgcttggttg ggcggccctg agcgaacgct ccgggccgtc gacctcgccc gcggcgagtt
      541 cgtggtgaag gagtgggtcg acggagtggt cacggaacgc cagatcaccg acgccgaact
      601 ggcggaggcc gacgatctgg tcgacgagta ccttgctgag gcagggatcc caccgaggcc
      661 aagaggctac ttgtggttca ccaccgtcga tctaggacac ctggaccgtg agttgagtcg
      721 ccgcattccc ggcaacacgg tcgtcctgcc gagaggcatg gcggagcaca tccgggcagt
      781 catcgaatcg gtgtgatggc cccaatcgcc gccgcttgtt gaaccgtgcg gctgtgactg
      841 cccagatcat ccggatctac tcgtcggctt ccgaagggcg gctgggagat ccggggcacc
      901 aaaatcccgg gtcacccagc tccttggtca ggatgtagcc gcactgtgcg acgccctccc
      961 agatcgcccg ggtgtcgaac gcttggcact tgccgttgtc ggccttcacc gctgaaagcg
     1021 ccgtctgcgg gtcgatgagc accgccagcc gcttgttgtg gtcctcgatc aggtcgccca
     1081 tccggttgcc ccagggcccg ggcactgaac cttcggcgcc caagtcccag ccgagcgcgt
     1141 cattggcttc gttcgccaag gcgtggaagc cggtgtagcc ggacgagacg gggggagcgg
     1201 gagccccacc ccgactaggc gttatgcctg gttgactagg caaaacgccg cagggcgaca
     1261 ggcgacctgc acccagaaga gaacttcaca tcgctcggtc ctccggtgtc cggatcgggc
     1321 cctttctgag gcttaaggct gcggaaggag ttcaccaggg aacagcagcg ctatgagtcc
     1381 ctggcccagg cggcggagcg gaccgggatc agccggaaga ccctgcgacg ccggatcgcg
     1441 tccggccaac tcccggtctc ctccagcgga cggcgcatcc tccgcgtgcg ccccgaggcc
     1501 gtcgacgcca tgctgcgtcc gttctggctc agttgagccc agaactagtg gtctggcgcg
     1561 gttttagctg actgcttggt caagggctgc tcgtgcgcgg gtgactttgg cgaggatgga
     1621 ttcggcggtg gcggtccagg tgtagggctt ggggttgtcg ttgctggcgt cgaggtattc
     1681 ctggatgctg tcgatcaggt cgggcacgga ggcgaagacg ccgcgccgta ggttcttctc
     1741 ggtgagatcg cggaaccagc gttcgacctg gttgagccat gacgacgagg tcggggtgaa
     1801 gtgcaggtgg aaccgcttgt gtcgtttgag ccaccgttcg acgctctcgt gcttgtgggt
     1861 ggcgtagttg tccaggatca ggtggacctg caggtgctgg ggaacctcgc ggtcgatggt
     1921 cttcaggaac gccaggaact cctcgtggcg gtgtcggggc aggcactggc cgatgaccgt
     1981 gccggtcagg acgtcgaggg cggcgaacaa ggtggtggtg ccgttgcgct tgtagtcatg
     2041 ggtcatcgtg ccggcccggc ccggcaccat cggcaacgag gcctgggtcc ggtccagtgc
     2101 ctggatctgg gacttctcgt ccatgcacaa cacgatcgcc ttctccggcg ggtccagata
     2161 cagcccgaca acgtcgatga gcttctcggt gaacgccgga tcgttggaca gcttgaacgt
     2221 gtccacccgg tgaggctgca aaccgagctc gttccagatc cgccgcaccg tgtccttgct
     2281 caccccggca cgctcagcca tcgtccgcgt cgaccagtgc gtcgcaccgg caggcttggt
     2341 cttggtggtg agttccacga tctcggcgac ctgctcctcg ctcacgctcg ccttccggcc
     2401 acgccccttg gcgaccttgc cccagttcgc caacccgtca gcctcgaacg cggcacgcca
     2461 ggaccgcacc gtcaccgcgg tcaccccgtg ccactgggcg atcgccaggt tcgcgcatcc
     2521 gtcagccgaa tcgagcagca cccgtgcccg gaccacttca cgatgggccg ccgtcgccga
     2581 gcgggccacc ttctcaagaa cctcacgctg aacaggcgac accctcaacg gtgcagcagc
     2641 aggagacggc atacctcatt ctacacccaa gtagacaccc atttgcgcgc cacaccacta
     2701 gggcatcccg gagaggtatc atttgggtat gactgatgtg gctaccatca aggttcctcg
     2761 cgtggtgcgt gaccgcgtgc gcgacgctgc ccgagcggcg cacctgaccc aggcccagct
     2821 catcgaggag atgcttgaag agaggcgcaa ggctgagttc tgggctgcgc tcgaagccga
     2881 gacccccgac caggagtatc tggacgacct tgccgaggca gacgcggcat ttgccggcga
     2941 cgccgaggag tccattgccc ggttcgaagc gggcgagtga tccagcagat tgcccgtggc
     3001 gatgtcgtct gggcgagcat ggacccgacc atcggccgcg agcaggccaa gcaccggcct
     3061 tggctggtgt tgtccgagcc ggcgctccat cgcgcccgcg gcctgctcat cgcggtgccg
     3121 ctgacgcaca ccgaccgggg atggtccacg cacgtgaagc tcacgtcccg cgattcggca
     3181 accaccgtgg cgatgtgcga acaggtcaag tcgatgtcta ttgaccgcgt cacccgcgtt
     3241 gaccgcgcgc cttacccgct cggccaggtc gatcaggtcc accagatcct cgcgctgctc
     3301 acaggcggcc gataggcgct actggaactg cagaggagca gcgccgagaa cgacgtggcc
     3361 ggcccggatc agctcgaccc ggcggtgcac ccggcccgcg atgctgtgca ctcccggcgc
     3421 atcctggcgg cccgacagg
//
LOCUS       DAOSUG010000341         3433 bp    DNA     linear   ENV 10-JUN-2024
DEFINITION  MAG TPA_asm: Micropruina sp. isolate AMR_MDS_2195
            NODE_11260_length_3433_cov_4.779751, whole genome shotgun sequence.
ACCESSION   DAOSUG010000341 DAOSUG010000000
VERSION     DAOSUG010000341.1
DBLINK      BioProject: PRJNA925477
            BioSample: SAMN32864262
            Sequence Read Archive: SRR4236673
KEYWORDS    WGS; ENV; Metagenome Assembled Genome; MAG; Third Party Data; TPA;
            TPA:assembly.
SOURCE      Micropruina sp. (wastewater metagenome)
  ORGANISM  Micropruina sp.
            Bacteria; Bacillati; Actinomycetota; Actinomycetes;
            Propionibacteriales; Nocardioidaceae; Micropruina.
REFERENCE   1  (bases 1 to 3433)
  AUTHORS   Abdulkadir,N., Saraiva,J.P., Zhang,J., Stolte,S., Gillor,O.,
            Harms,H. and Rocha,U.
  TITLE     Genome-centric analyses of 165 metagenomes show that mobile genetic
            elements are crucial for the transmission of antimicrobial
            resistance genes to pathogens in activated sludge and wastewater
  JOURNAL   Microbiol Spectr 12 (3), e0291823 (2024)
   PUBMED   38289113
REFERENCE   2  (bases 1 to 3433)
  AUTHORS   Abdulkadir,N.
  TITLE     Direct Submission
  JOURNAL   Submitted (24-MAR-2023) Environmental Microbiology, Helmholtz
            Center for Environmental Research -UFZ, Permoserstr 15, Leipzig
            04318, Germany
COMMENT     The annotation was added by the NCBI Prokaryotic Genome Annotation
            Pipeline (PGAP). Information about PGAP can be found here:
            https://www.ncbi.nlm.nih.gov/genome/annotation_prok/
            
            ##Genome-Assembly-Data-START##
            Assembly Method        :: SPAdes v. 3.11.1
            Genome Representation  :: Full
            Expected Final Version :: Yes
            Genome Coverage        :: Not Applicable
            Sequencing Technology  :: Illumina MiSeq
            ##Genome-Assembly-Data-END##
            
            ##Genome-Annotation-Data-START##
            Annotation Provider               :: NCBI
            Annotation Date                   :: 05/04/2023 04:57:10
            Annotation Pipeline               :: NCBI Prokaryotic Genome
                                                 Annotation Pipeline (PGAP)
            Annotation Method                 :: Best-placed reference protein
                                                 set; GeneMarkS-2+
            Annotation Software revision      :: 6.5
            Features Annotated                :: Gene; CDS; rRNA; tRNA; ncRNA
            Genes (total)                     :: 2,776
            CDSs (total)                      :: 2,750
            Genes (coding)                    :: 2,714
            CDSs (with protein)               :: 2,714
            Genes (RNA)                       :: 26
            tRNAs                             :: 24
            ncRNAs                            :: 2
            Pseudo Genes (total)              :: 36
            CDSs (without protein)            :: 36
            Pseudo Genes (ambiguous residues) :: 0 of 36
            Pseudo Genes (frameshifted)       :: 2 of 36
            Pseudo Genes (incomplete)         :: 32 of 36
            Pseudo Genes (internal stop)      :: 2 of 36
            ##Genome-Annotation-Data-END##
FEATURES             Location/Qualifiers
     source          1..3433
                     /organism="Micropruina sp."
                     /mol_type="genomic DNA"
                     /submitter_seqid="NODE_11260_length_3433_cov_4.779751"
                     /isolate="AMR_MDS_2195"
                     /isolation_source="Activated sludge of municipal
                     wastewater treatment plants"
                     /db_xref="taxon:2737536"
                     /environmental_sample
                     /geo_loc_name="Canada"
                     /lat_lon="49.25 N 123.1 W"
                     /collection_date="2015-06-16"
                     /metagenome_source="wastewater metagenome"
                     /note="metagenomic"
     gene            complement(<1..816)
                     /locus_tag="PLF56_10685"
     CDS             complement(<1..816)
                     /locus_tag="PLF56_10685"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_008355982.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_component: GO:0016020 - membrane [Evidence IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="low temperature requirement protein A"
                     /protein_id="HOQ54064.1"
                     /translation="MSAAPGPPRPISKRPSPLRRMSGRDVHEPHRAATPLELLMDLAF
                     VVAFGIAGQNVAHLIAEGHVAAGLGGFAFAMFAIVWAWINFSWFASAFDTDDWAYRLT
                     TMLQMIGVVIFSLGLPDVFYSLDEGMQLDNRVIIAGYVVMRVAMLTQWLRVANSEPRY
                     RTTALTYAAAIAVAQVGWVVVAWLDLSLPAALTGAGVLIVVEMLGPFIAERRHTPTPW
                     HPHHIAERYGLLVIITLGEGVIGTVAAVGSAVGEEGWTFEVIAVVVAGIGLTFG"
     gene            1029..1883
                     /locus_tag="PLF56_10690"
     CDS             1029..1883
                     /locus_tag="PLF56_10690"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_012922765.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="transporter substrate-binding domain-containing
                     protein"
                     /protein_id="HOQ54065.1"
                     /translation="MSFRPKALRAALAGVAALSLSMLAACANYSTPTTAVPAGTGTGG
                     GTAAAAPTLVKDGKLTVCTHLAYKPFQFKDDSGKTVGFDVDLMDLVAKKLGVTQEIFD
                     IEFEQITSGAVFAAKKCDAAAAAITIRADRQEAALFSDPYFSATQALLVSSDSPIADL
                     PDLKGKTLAVQAGTTGQDYAKEKAAANGFTVKVFDDMPSGANAVLAKTVDAAINDNGV
                     VYNFASENPGTKVVKEFDTNEKYGFSMGKTNTALLTVVNDVLKTAKTDGTYNTIYKKW
                     FGKDAPKD"
     gene            1956..2774
                     /locus_tag="PLF56_10695"
     CDS             1956..2774
                     /locus_tag="PLF56_10695"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_008362888.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_function: GO:0042626 - ATPase-coupled transmembrane
                     transporter activity [Evidence IEA];
                     GO_function: GO:0140359 - ABC-type transporter activity
                     [Evidence IEA];
                     GO_process: GO:0006865 - amino acid transport [Evidence
                     IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="amino acid ABC transporter permease"
                     /protein_id="HOQ54066.1"
                     /translation="MTSTATPTRKKLSPRRRAQRIRWVNYGVLVLALLAVVLFADGRQ
                     LQSVFFRPDMIAETVGPGLLNALKNTILYTVGAFVFGLALGTALALMKLSQIGPYRWI
                     ATIYTEVFRGVPAIIVLLAFSLISLAIPGLQIPFDPFGAVWTALGLVAAAYMSETIRA
                     GIQAVPKGQTEAARSLGMPSGMATRKIVLPQAFRIVTPPLTNELVSLTKDSSLIYVIG
                     LSAEAFDLTKYGRDLTNTHSNMTPLVIAGLTYLVITLPLTFLVRRMEAKATKAR"
     gene            2780..>3433
                     /locus_tag="PLF56_10700"
     CDS             2780..>3433
                     /locus_tag="PLF56_10700"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_012922767.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_function: GO:0005524 - ATP binding [Evidence IEA];
                     GO_function: GO:0016887 - ATP hydrolysis activity
                     [Evidence IEA];
                     GO_function: GO:0042626 - ATPase-coupled transmembrane
                     transporter activity [Evidence IEA];
                     GO_function: GO:0140359 - ABC-type transporter activity
                     [Evidence IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="amino acid ABC transporter ATP-binding protein"
                     /protein_id="HOQ54067.1"
                     /translation="MTQTIPTGNRDDEIQIYDLHKSFGQIEVLKGISTIIRRGEVVCV
                     IGPSGSGKSTLLRCVNLLEQPTAGRIFILNEEITDPDAFVDELRTKMGMVFQQFNLFP
                     HLTALQNCTVAPVNVLKKEKATAEATARKNLAMVGLADRGDAYPAQLSGGQQQRVAIA
                     RALSMDPELMLFDEPTSALDPELVGDVLSVMRELAKSGMTMMVVTHEMAFARDVADRV
                     "
BASE COUNT          642 a         1170 c         1060 g          561 t
ORIGIN      
        1 cccgaacgtg agcccgatgc cggccaccac caccgcgatc acctcgaacg tccagccctc
       61 ttcgccgacg gccgacccga ccgccgcgac cgtgccgatc acgccctcgc ccagcgtgat
      121 gatcaccagc agcccgtagc gttcggcgat gtggtgcggg tgccaggggg tgggggtgtg
      181 gcgccgctcg gcgatgaacg gcccgagcat ctcgaccacg atcagcaccc ccgcgccggt
      241 cagggcggcc ggcagcgaca ggtcgagcca ggccaccacc acccagccca cctgggcgac
      301 cgcgatggcg gcggcgtagg tgagcgcggt ggtgcggtag cgcggctcgg agttggcgac
      361 ccgcagccac tgggtgagca tcgccactcg catcaccacg tagccggcga tgatcacccg
      421 gttgtccagc tgcatgccct cgtccagcga ataaaagacg tccggaaggc cgaggctgaa
      481 gatcaccacg ccgatcatct gcagcatcgt ggtcagtcgg tacgcccagt cgtcggtatc
      541 gaaggccgac gcgaaccagc tgaagttgat ccacgcccac acgatggcga acatcgcgaa
      601 ggcgaagccg cccagtccgg ccgccacgtg cccctcggcg atcaggtgcg ccacattctg
      661 gcccgcgatc ccgaaggcca ccacgaacgc caggtccatc agcagttcga gcggggtggc
      721 ggcgcgatgc ggctcgtgca cgtcgcgtcc gctcatccgg cgcagcggcg agggtcgctt
      781 gctgatcgga cggggcgggc ctggcgcagc actcatggcg cgacacccta acccgtttgc
      841 aacgatcacc ccgctgcccg accgcatccc ggcgcccggg cgcgtcgggc atgctcacca
      901 gtactgacgg accgggttct tggcgcccgt gaaggtcaca agggggtctc gatcggcgtt
      961 ccgcattgcc gccgtaacaa acctgcgaca ggatgcggcc aacctgatag ctctatcaag
     1021 gagaagaagt gagttttcga cccaaggccc tgcgtgcggc actcgccggc gtcgcggccc
     1081 tcagcctcag catgctcgcc gcgtgcgcca actactccac cccgaccacc gcggtcccgg
     1141 ccggtaccgg aacgggtggc ggcaccgctg cagcagcacc gaccctggtg aaagacggca
     1201 agctgaccgt gtgcacccac ctcgcctaca agccgttcca gttcaaggac gactcgggca
     1261 agaccgtcgg attcgacgtc gacctgatgg acctggtggc caagaagctg ggtgtgactc
     1321 aggagatttt cgacatcgag ttcgagcaga tcacctcggg cgcggtgttc gccgccaaga
     1381 agtgtgacgc ggcggctgcg gcaatcacga tccgggccga tcgtcaagag gccgcactgt
     1441 tctccgaccc ctacttctcc gccacgcagg cattgctggt gagcagcgat tcgcccatcg
     1501 ctgacctgcc cgacctcaag ggcaagaccc tggccgttca agccggaacc accggtcagg
     1561 actacgccaa agagaaggcc gcggccaacg gtttcaccgt caaggtgttc gacgacatgc
     1621 cgagcggtgc caatgcggtg ctggcgaaga cagtcgacgc tgctatcaac gacaacggcg
     1681 ttgtgtacaa cttcgcgtcg gagaatcccg gcaccaaggt ggtcaaagag ttcgacacca
     1741 acgagaagta cgggttcagc atgggcaaga cgaacaccgc gctgctgacg gtggtcaacg
     1801 acgtgctgaa gacggccaag accgacggca cctacaacac catctacaag aagtggttcg
     1861 gcaaggacgc accgaaggac tgactccact cacgctttgc gctgccccgg tctgtcggcc
     1921 ggggcagccg ggcgccgttc cgcaagggag accgcatgac gtccactgcc acaccgacac
     1981 gcaagaagtt gtcgccgcgc cgccgcgccc agcgcatccg ttgggtcaat tacggcgttc
     2041 tcgtcctggc gctcctggct gtcgtcctct tcgccgacgg acggcagttg cagtccgtct
     2101 tcttccggcc cgacatgatc gccgagaccg tcgggcccgg cctgctcaac gcactcaaga
     2161 acaccatcct gtataccgtc ggagccttcg tcttcggcct ggcgctgggc accgctcttg
     2221 ccctgatgaa gctttcgcag atcggtccct accgatggat cgcgaccatc tacaccgagg
     2281 ttttccgggg tgtcccggcc atcatcgtgc tgctggcctt cagcctcatt tcgctggcca
     2341 tacccggact gcagattccg ttcgacccgt tcggtgcggt ctggacggcc ctcggcctgg
     2401 ttgccgcggc atacatgtcg gagaccatcc gggccggtat tcaggcggtc cccaagggtc
     2461 agaccgaagc ggcacgatcg ctcggcatgc cgtccggaat ggcgactcga aagatcgttc
     2521 tgccgcaagc gttccgcatc gtcacgccgc cactcaccaa cgaactggtt tcgttgacca
     2581 aggattcgtc cctgatctat gtgatcgggc tgagcgcgga ggcgttcgac ctcaccaagt
     2641 acggccgcga cctgaccaac acccactcga acatgacgcc actggtgatc gctggcttga
     2701 cctacctggt catcacgctt ccgctcacct tcttggtgcg gcgcatggaa gccaaagcca
     2761 cgaaggcccg gtgaacgcca tgacccagac aattcccacc ggcaaccgtg acgacgagat
     2821 ccagatctac gacctgcaca agagcttcgg tcagatcgag gtcctgaaag gcatctcgac
     2881 cattattcga cgcggcgagg tggtgtgcgt gatcgggccg tccggctccg gaaagtccac
     2941 gctgttgcgc tgtgtgaacc tgctggaaca gccgaccgcc ggtcgcatct tcattctgaa
     3001 cgaagagatc accgacccgg acgccttcgt cgacgagctg cgtaccaaga tgggcatggt
     3061 ctttcaacag ttcaacctgt ttccgcatct gacggcactg cagaactgca ccgtcgcgcc
     3121 ggtcaacgtg ttgaagaagg agaaggcgac cgccgaggcg acggcccgca agaatctcgc
     3181 catggtcggg ctcgccgacc ggggtgacgc gtaccccgcg caactctccg gaggccagca
     3241 gcagcgggtg gcgatcgccc gggccctgag catggacccc gagttgatgc tgttcgacga
     3301 gcccacctcg gcgctcgatc ccgagctggt cggcgacgtg ctgtcggtga tgcgcgagtt
     3361 ggcgaagtcc ggcatgacca tgatggtggt cacccacgag atggccttcg cccgcgacgt
     3421 tgccgaccgg gtg
//
LOCUS       DAOSUG010000342         3433 bp    DNA     linear   ENV 10-JUN-2024
DEFINITION  MAG TPA_asm: Micropruina sp. isolate AMR_MDS_2195
            NODE_11261_length_3433_cov_3.986679, whole genome shotgun sequence.
ACCESSION   DAOSUG010000342 DAOSUG010000000
VERSION     DAOSUG010000342.1
DBLINK      BioProject: PRJNA925477
            BioSample: SAMN32864262
            Sequence Read Archive: SRR4236673
KEYWORDS    WGS; ENV; Metagenome Assembled Genome; MAG; Third Party Data; TPA;
            TPA:assembly.
SOURCE      Micropruina sp. (wastewater metagenome)
  ORGANISM  Micropruina sp.
            Bacteria; Bacillati; Actinomycetota; Actinomycetes;
            Propionibacteriales; Nocardioidaceae; Micropruina.
REFERENCE   1  (bases 1 to 3433)
  AUTHORS   Abdulkadir,N., Saraiva,J.P., Zhang,J., Stolte,S., Gillor,O.,
            Harms,H. and Rocha,U.
  TITLE     Genome-centric analyses of 165 metagenomes show that mobile genetic
            elements are crucial for the transmission of antimicrobial
            resistance genes to pathogens in activated sludge and wastewater
  JOURNAL   Microbiol Spectr 12 (3), e0291823 (2024)
   PUBMED   38289113
REFERENCE   2  (bases 1 to 3433)
  AUTHORS   Abdulkadir,N.
  TITLE     Direct Submission
  JOURNAL   Submitted (24-MAR-2023) Environmental Microbiology, Helmholtz
            Center for Environmental Research -UFZ, Permoserstr 15, Leipzig
            04318, Germany
COMMENT     The annotation was added by the NCBI Prokaryotic Genome Annotation
            Pipeline (PGAP). Information about PGAP can be found here:
            https://www.ncbi.nlm.nih.gov/genome/annotation_prok/
            
            ##Genome-Assembly-Data-START##
            Assembly Method        :: SPAdes v. 3.11.1
            Genome Representation  :: Full
            Expected Final Version :: Yes
            Genome Coverage        :: Not Applicable
            Sequencing Technology  :: Illumina MiSeq
            ##Genome-Assembly-Data-END##
            
            ##Genome-Annotation-Data-START##
            Annotation Provider               :: NCBI
            Annotation Date                   :: 05/04/2023 04:57:10
            Annotation Pipeline               :: NCBI Prokaryotic Genome
                                                 Annotation Pipeline (PGAP)
            Annotation Method                 :: Best-placed reference protein
                                                 set; GeneMarkS-2+
            Annotation Software revision      :: 6.5
            Features Annotated                :: Gene; CDS; rRNA; tRNA; ncRNA
            Genes (total)                     :: 2,776
            CDSs (total)                      :: 2,750
            Genes (coding)                    :: 2,714
            CDSs (with protein)               :: 2,714
            Genes (RNA)                       :: 26
            tRNAs                             :: 24
            ncRNAs                            :: 2
            Pseudo Genes (total)              :: 36
            CDSs (without protein)            :: 36
            Pseudo Genes (ambiguous residues) :: 0 of 36
            Pseudo Genes (frameshifted)       :: 2 of 36
            Pseudo Genes (incomplete)         :: 32 of 36
            Pseudo Genes (internal stop)      :: 2 of 36
            ##Genome-Annotation-Data-END##
FEATURES             Location/Qualifiers
     source          1..3433
                     /organism="Micropruina sp."
                     /mol_type="genomic DNA"
                     /submitter_seqid="NODE_11261_length_3433_cov_3.986679"
                     /isolate="AMR_MDS_2195"
                     /isolation_source="Activated sludge of municipal
                     wastewater treatment plants"
                     /db_xref="taxon:2737536"
                     /environmental_sample
                     /geo_loc_name="Canada"
                     /lat_lon="49.25 N 123.1 W"
                     /collection_date="2015-06-16"
                     /metagenome_source="wastewater metagenome"
                     /note="metagenomic"
     gene            complement(323..592)
                     /locus_tag="PLF56_10705"
     CDS             complement(323..592)
                     /locus_tag="PLF56_10705"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_013865048.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="DUF2277 domain-containing protein"
                     /protein_id="HOQ54068.1"
                     /translation="MCRNIRVLNNFEPPATNDEVHAAALQFVRKVSGMNKPSKANEPY
                     FNEAVDEIAHLTRHLLEHLTTAAPPKNREEEADKARARSAARFAS"
     gene            678..1595
                     /locus_tag="PLF56_10710"
     CDS             678..1595
                     /locus_tag="PLF56_10710"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_014846505.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="phosphodiester glycosidase family protein"
                     /protein_id="HOQ54069.1"
                     /translation="MSDSLLTKKVSRRLLFGGTAGLVGLGGTAAWAADRFLVEHVEGT
                     VTDVSTATPTGTSTDNGYTSATTKLTIEKVATGSGSDAITYFVADITTTDATLIRSAF
                     AKDEFGTNIIAYPSVIAKSKNAVLAINGDYYGFRDTGIVVRGGVAYRDKGARQGLAMY
                     RDGAMRLYDETATTAKQLIADGVWNTLSFGPGLVADGAVIDGIDQVEIDTNVGNHSIQ
                     GNQPRTAVGMVAAGHFLWVVVDGRSSGYSRGMTLPELAQVFVDRGAQVAYNLDGGGSS
                     AMVFNGSLVNNPLGKGQERGTSDILYVAG"
     gene            1599..2657
                     /locus_tag="PLF56_10715"
     CDS             1599..2657
                     /locus_tag="PLF56_10715"
                     /inference="COORDINATES: protein motif:HMM:NF016060.2"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="bifunctional glycosyltransferase family 2/GtrA
                     family protein"
                     /protein_id="HOQ54070.1"
                     /translation="MIVLIPAYEPGRCLLELIRDLAREAPELQVVVVDDGSGPLYAPL
                     FSDVSRLGADVIGYAANRGKGAALKHGFAHIARHYPDQSVVCADSDGQHAVVDILRVG
                     RAVGPGSMVLGVRAFSGRVPLRSRVGNSLTRWAFRAASGVGVSDTQTGLRGYPADALP
                     WLGTVSGDRFEYELNLLLQARSAGWTIVEVPIATIYLAGNASSHFRPLTDSARIYLPL
                     LAFSASSLVAFAIDFVALLLLNAVTGSLLVAVIGARGLSSAVNFVTNRRVVFESRRPL
                     RSTLVRYYLLVGVLLLANYGLLAWLTSFGLALALAKMLVEVTLFVASYQVQRSFVFGL
                     QPPPVTAVAAPRERLPIG"
     gene            complement(2754..3194)
                     /locus_tag="PLF56_10720"
     CDS             complement(2754..3194)
                     /locus_tag="PLF56_10720"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_011754850.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="CoA-binding protein"
                     /protein_id="HOQ54071.1"
                     /translation="MIHPSTTQIRPAWQSQQVIDSFLDDAHVWAVVGLSGDPSRTAYA
                     IADLLQSRGKQVVPIHPDAPVVFGQQGYRSLAEVPFDIDVVDVFRRSESAGAFVDEAL
                     AVGAKGVWFQLGVIDHDAFERAQTAGLPMVMDTCPAIEWRLRES"
BASE COUNT          536 a         1142 c         1191 g          564 t
ORIGIN      
        1 gtcaagcagg gcgacgacgt cacggtggtc gagatccggc acgagaacat tccgaccggc
       61 tggaacaccg cgtcgatcac cgagcattgg gagcatgcgc tcgaccgaat cgtcgacgcg
      121 agctgagctg agttcgtagc gcccgacggg gcccaccggc aggtgcggcc ccgtccgcgt
      181 ttcatcgggc ctggaagcct gctgcacagt cattttccgg ctgcggcgca tgggcgggcg
      241 atccgctgcg gtcacatcgt ggctcggtag ccaggccagg gccgcgacgg ggaccggtcg
      301 acgcctcatc gacccgcggt gctcagctcg cgaagcgcgc cgctgaccgg gctcgtgcct
      361 tgtccgcttc ctcctcgcgg ttcttcggcg gggccgccgt ggtcaggtgt tccagcaggt
      421 gccgcgtgag gtgcgcgatc tcgtcgaccg cctcgttgaa gtacggctcg ttggccttgg
      481 acggcttgtt catgcccgac accttgcgca cgaactgcaa cgcggcggcg tgcacctcgt
      541 cattggtggc cggcggctcg aagttgttca gaactcgaat gttgcgacac atggcgacca
      601 gcgtaagtga gaaatcgccc agcgcgaccg cagccccggc acagaagcag cacaggcggc
      661 gtccgtacaa ctgagttgtg agcgattctc tcctgaccaa gaaggtgtca cggcggctgc
      721 tgttcggcgg cacggccggg ctggtcggcc tgggcggcac ggcggcctgg gcggcggacc
      781 gctttctggt cgagcacgtc gagggcaccg tgaccgacgt cagcacggcc acgccgaccg
      841 gcaccagcac cgacaacggc tacaccagcg cgacgacgaa gctgaccatc gagaaggttg
      901 ccaccggctc gggcagcgac gcgatcacct acttcgtcgc cgacatcacc acgacggacg
      961 caacactgat ccgctcagcg ttcgcgaagg acgagttcgg caccaacatc atcgcctacc
     1021 cgtcggtgat cgcgaagagc aagaacgccg tgctcgcgat caacggcgac tactacgggt
     1081 ttcgcgacac cggcatcgtg gtgcgcggcg gagtcgccta ccgcgacaag ggcgccaggc
     1141 aaggtctggc catgtatcgc gacggcgcca tgcggctgta cgacgagacc gcaaccaccg
     1201 ccaagcagct gatcgccgac ggcgtgtgga acacgctttc gttcggtccc ggcctggtcg
     1261 cggacggcgc ggtgatcgac ggcatcgacc aggtcgagat cgacaccaac gtgggcaacc
     1321 actccattca gggcaaccag ccgcggactg ccgttggcat ggtcgccgcc ggccactttc
     1381 tgtgggtggt ggtggacggc cgcagctccg gctacagccg cggcatgacc ctgccggaac
     1441 tcgcgcaggt cttcgtcgac cgcggcgcgc aggtcgccta caacctggac gggggcggat
     1501 cgtccgccat ggtgttcaac ggctcgctgg tcaacaatcc gctcggcaag ggccaggagc
     1561 gcggcacgtc cgacatcctc tacgtggcgg gctgagcgat gatcgtgctg attcccgcct
     1621 atgagcccgg acgctgcctg ctggagttga tccgtgacct ggcccgcgag gcgccggagt
     1681 tgcaggtcgt ggtggtggac gacggcagcg gcccgctgta cgccccgctg ttctcggacg
     1741 tcagccggct gggtgccgac gtgatcggct acgcggccaa ccggggcaag ggggcggcgc
     1801 tcaagcacgg cttcgcacat atcgcacggc actatcccga ccagagcgtg gtctgtgccg
     1861 attccgacgg tcagcacgcg gtggtcgaca ttcttcgggt cggacgtgcg gtaggtccag
     1921 gcagcatggt gctgggcgtc cgggccttca gcggacgggt gcccctgcgc agccgcgtcg
     1981 gcaacagcct cacccgatgg gcgttccgcg ccgccagcgg ggtgggggtc agcgacaccc
     2041 agaccggcct gcgcggctac ccggccgacg cgttgccctg gctcggcacg gtcagcggcg
     2101 accggttcga gtacgaactg aacctgctac tgcaggctcg cagcgccgga tggacgatcg
     2161 tcgaggtgcc gatcgccacc atctacctgg cgggcaacgc atcgtcgcat tttcggccgc
     2221 tgaccgattc ggcccgcatc tacctgccgt tgctggcgtt ctcggcctcg tcgctggtcg
     2281 cgttcgcgat cgatttcgtg gccctcctgc tgctgaacgc ggtgacgggc agcctgctgg
     2341 tggccgtgat cggcgcgcgc gggctcagtt cggcggtcaa cttcgtgacc aatcggcggg
     2401 tggtgttcga gtcgcggcgc ccgctgcgca gcacgctggt gcgctactac ctgctggtag
     2461 gcgtgttgct gctcgccaac tacggcctgc tggcgtggct gacctcgttc gggctggccc
     2521 tggcgttggc gaagatgctg gtcgaggtga cgttgttcgt ggccagctat caggtgcagc
     2581 gcagcttcgt gttcggcctc caacccccgc cggtgacggc ggtggcagca ccgcgcgagc
     2641 ggctgccgat cggctgactc ggccggacgg accgatggcc accatcgcaa gcgcaccgaa
     2701 cgggtgggtg cccggacggg gtcgcgcaac agcggcaatc ggtggatgcc gtctcagctc
     2761 tcgcggagcc gccattcgat ggccgggcag gtgtccatca ccatcggcaa tccggcggtc
     2821 tgggcccgtt cgaacgcgtc gtggtcgatc acgccgagct ggaaccagac gcccttggcg
     2881 ccgaccgcca gcgcttcgtc cacgaacgcc ccggccgatt cggaacgccg gaacacgtcg
     2941 accacgtcga tgtcgaacgg cacctcggcg aggctgcggt agccctgctg gccgaaaacc
     3001 acaggggcat cgggatggat cggaacgacc tgcttgcccc ggctctgtag aaggtcggcg
     3061 atggcgtagg cggttcggct ggggtcgccg gacaatccga ccacggccca cacgtgggcg
     3121 tcatcgagaa agctgtcgat cacctgctgg ctctgccagg caggacggat ctgggtggtg
     3181 ctggggtgga tcatgccaac aaccctaacc aacgtgggcg cacgagtatt cacggcaatg
     3241 ggtgaggcgg ccgttcgcga gtcgggaccg gccgaaccgt cgtggcgccc gtcatcccgg
     3301 cgcatgccgg gatctctgcg tgagccgaga tcccggggca agcccgggat gacgagtgag
     3361 cggcttgtgg agacccctgc tcgcctcggt ttcggccagc tcgtccagcg attcccgatc
     3421 ccgtcatccc ggc
//
LOCUS       DAOSUG010000343         3433 bp    DNA     linear   ENV 10-JUN-2024
DEFINITION  MAG TPA_asm: Micropruina sp. isolate AMR_MDS_2195
            NODE_11262_length_3433_cov_3.888692, whole genome shotgun sequence.
ACCESSION   DAOSUG010000343 DAOSUG010000000
VERSION     DAOSUG010000343.1
DBLINK      BioProject: PRJNA925477
            BioSample: SAMN32864262
            Sequence Read Archive: SRR4236673
KEYWORDS    WGS; ENV; Metagenome Assembled Genome; MAG; Third Party Data; TPA;
            TPA:assembly.
SOURCE      Micropruina sp. (wastewater metagenome)
  ORGANISM  Micropruina sp.
            Bacteria; Bacillati; Actinomycetota; Actinomycetes;
            Propionibacteriales; Nocardioidaceae; Micropruina.
REFERENCE   1  (bases 1 to 3433)
  AUTHORS   Abdulkadir,N., Saraiva,J.P., Zhang,J., Stolte,S., Gillor,O.,
            Harms,H. and Rocha,U.
  TITLE     Genome-centric analyses of 165 metagenomes show that mobile genetic
            elements are crucial for the transmission of antimicrobial
            resistance genes to pathogens in activated sludge and wastewater
  JOURNAL   Microbiol Spectr 12 (3), e0291823 (2024)
   PUBMED   38289113
REFERENCE   2  (bases 1 to 3433)
  AUTHORS   Abdulkadir,N.
  TITLE     Direct Submission
  JOURNAL   Submitted (24-MAR-2023) Environmental Microbiology, Helmholtz
            Center for Environmental Research -UFZ, Permoserstr 15, Leipzig
            04318, Germany
COMMENT     The annotation was added by the NCBI Prokaryotic Genome Annotation
            Pipeline (PGAP). Information about PGAP can be found here:
            https://www.ncbi.nlm.nih.gov/genome/annotation_prok/
            
            ##Genome-Assembly-Data-START##
            Assembly Method        :: SPAdes v. 3.11.1
            Genome Representation  :: Full
            Expected Final Version :: Yes
            Genome Coverage        :: Not Applicable
            Sequencing Technology  :: Illumina MiSeq
            ##Genome-Assembly-Data-END##
            
            ##Genome-Annotation-Data-START##
            Annotation Provider               :: NCBI
            Annotation Date                   :: 05/04/2023 04:57:10
            Annotation Pipeline               :: NCBI Prokaryotic Genome
                                                 Annotation Pipeline (PGAP)
            Annotation Method                 :: Best-placed reference protein
                                                 set; GeneMarkS-2+
            Annotation Software revision      :: 6.5
            Features Annotated                :: Gene; CDS; rRNA; tRNA; ncRNA
            Genes (total)                     :: 2,776
            CDSs (total)                      :: 2,750
            Genes (coding)                    :: 2,714
            CDSs (with protein)               :: 2,714
            Genes (RNA)                       :: 26
            tRNAs                             :: 24
            ncRNAs                            :: 2
            Pseudo Genes (total)              :: 36
            CDSs (without protein)            :: 36
            Pseudo Genes (ambiguous residues) :: 0 of 36
            Pseudo Genes (frameshifted)       :: 2 of 36
            Pseudo Genes (incomplete)         :: 32 of 36
            Pseudo Genes (internal stop)      :: 2 of 36
            ##Genome-Annotation-Data-END##
FEATURES             Location/Qualifiers
     source          1..3433
                     /organism="Micropruina sp."
                     /mol_type="genomic DNA"
                     /submitter_seqid="NODE_11262_length_3433_cov_3.888692"
                     /isolate="AMR_MDS_2195"
                     /isolation_source="Activated sludge of municipal
                     wastewater treatment plants"
                     /db_xref="taxon:2737536"
                     /environmental_sample
                     /geo_loc_name="Canada"
                     /lat_lon="49.25 N 123.1 W"
                     /collection_date="2015-06-16"
                     /metagenome_source="wastewater metagenome"
                     /note="metagenomic"
     gene            <1..1532
                     /locus_tag="PLF56_10725"
     CDS             <1..1532
                     /locus_tag="PLF56_10725"
                     /inference="COORDINATES: protein motif:HMM:NF012789.2"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_function: GO:0005524 - ATP binding [Evidence IEA]"
                     /codon_start=3
                     /transl_table=11
                     /product="UvrD-helicase domain-containing protein"
                     /protein_id="HOQ54072.1"
                     /translation="TVVALHRAAYLLYSNRRRFERGGILVVGPSSLYMNYIERVLPSL
                     GEDSVTLRAIGAVASDALGLVADRNDDAAATGIKGSLRMAPLLQRLVNLPLIDPGPDG
                     VRLRVTVKGEVLGLEGPALARIRAEVLARHKVNRGRAPAEQALLKALVAQVPDEIDLE
                     RDVVEDLITSSAQWRMFVNAWWPALAPTEVLRRLDDPAIVRQVAVGLLNADEQRALQQ
                     SFTADTWSVADIALLDELAALLGPVPVDDDADQPLFIEGGEAVPELITMADRLSRERQ
                     DDADGEGHDEFAHLLIDEAQDITPMQWRMLRRRGGHASWTIVGDPAQSSWPDLAESER
                     AIAELIGHAPNRTYRLSVNYRSPAEVFSLAAQVVRRSFPEADLPKAVRSTGAQPQLVG
                     TEPESFLRVVAEQAARLLDQVAGTVGVIAPPSRFEAVRATLEPLADNRLVVVTPLQAK
                     GLEYDGVLVAAPDDIVAESPGGERVLYVALTRPTQLLVTVDPVPAGAGWRESLTLPVT
                     G"
     gene            complement(1441..2394)
                     /locus_tag="PLF56_10730"
     CDS             complement(1441..2394)
                     /locus_tag="PLF56_10730"
                     /EC_number="1.5.1.20"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_012920479.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_function: GO:0004489 - methylenetetrahydrofolate
                     reductase (NAD(P)H) activity [Evidence IEA];
                     GO_process: GO:0006555 - methionine metabolic process
                     [Evidence IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="methylenetetrahydrofolate reductase"
                     /protein_id="HOQ54073.1"
                     /translation="MSVTPDDVAAQEHLPSIADLLADTSRPTFSFEFFPPADVAGEDV
                     LAQTILDLDATGADWLSVTYGASGATRDRTIRATRFIKRRTEAQTMGHLTCASQSRDE
                     LLATLAAYAEADVRHVLAIRGDMPGGPTVPWQRHPDGLANATELVRLIAGNFSFGIGV
                     AAFPDPHPQQHDADLDARLLLAKQEAGASFAVTQLFFDADAYLRLVERARAIGCTLPI
                     VAGIQPVTNIAQIERFAALSGAELPTRVVRRLHAVAGDPAQVRRVGIEIATELSADVL
                     RGGAPGLQFFTLNRSRATSAILANLRRLGLDRRSPAAASDA"
     gene            2446..>3433
                     /locus_tag="PLF56_10735"
     CDS             2446..>3433
                     /locus_tag="PLF56_10735"
                     /inference="COORDINATES: protein motif:HMM:NF012568.2"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="polyprenyl synthetase family protein"
                     /protein_id="HOQ54074.1"
                     /translation="MGIFAAHDPLGAPFRDAMGAHLRTFIEARREQLTAVGPQLRALT
                     DLALAFTAGGKRLRPAFCWWGYCSIVEPDDAEALLRAAASLDLLHVSALVHDDVMDSS
                     DTRRGLAAAHRQFETWHHEGGLLGSPEAFGRAGAILLGDLLLVWSMEAFTGSGLPMAA
                     LARATPLLDAMRTEVTCGQFLDVFAQAEPVGGVDLMSLVPQVVEFKTSRYTVIRPLQV
                     GAALAGASEPALAALAAYGSPLGRAFQFRDDVLGVFGEPEVTGKPAGDDLREGKRTVL
                     VAHALELASETAKERLIGLLGSSALDATDIATAREIIVQSGALAATEQRITDD"
BASE COUNT          484 a         1151 c         1273 g          525 t
ORIGIN      
        1 agaccgtcgt cgccctgcat cgcgccgcct acttgctgta cagcaaccgg cgccgcttcg
       61 agcgcggcgg catcctggtg gtcggcccgt cgtcgctgta catgaactac atcgagcggg
      121 tgctgccctc actgggcgag gattcggtca ccctgcgtgc gatcggagcc gtggccagcg
      181 acgcgcttgg gctggtcgcc gaccgcaacg acgatgccgc cgccaccggg atcaagggct
      241 cactgcgaat ggcccccctg ctgcagcggc tggtcaacct gccgctgatc gatcccgggc
      301 cggacggagt tcggctgcgg gtcaccgtca agggcgaggt gctgggcctg gaggggccgg
      361 cgttggcccg gatccgggcc gaggttctgg cccgccacaa ggtcaatcgg ggccgggcgc
      421 ccgctgagca ggcgttgctg aaggctctgg tggcgcaggt gcccgacgag atcgacctcg
      481 agcgtgacgt cgtcgaggac ctgatcacgt ccagcgccca gtggcgcatg ttcgtcaacg
      541 cctggtggcc cgcactggcc cccaccgagg tgttgcgccg actggacgac cccgcgatcg
      601 tccggcaggt ggcggtcggg ctgctgaacg ctgacgagca acgggccctg cagcagtcat
      661 tcaccgccga cacctggtcg gtggccgaca tcgccctgct cgacgagctg gcggcactgc
      721 tcgggcccgt gcccgtggac gacgacgcgg accagccgct gttcatcgag ggcggcgagg
      781 cggtgcccga actgatcacg atggccgacc ggctctcacg cgagcgccag gacgacgccg
      841 acggcgaggg ccacgacgag ttcgcccacc tgctgatcga cgaggcgcag gacatcaccc
      901 cgatgcagtg gcgcatgctg cgccgccggg gcgggcacgc gtcctggacc atcgtcggcg
      961 accccgccca gagcagttgg cccgatctcg ccgagtcgga gcgcgcgatc gctgaactga
     1021 tcggccacgc ccccaaccgc acttaccggc tgagcgtcaa ctaccgctcc cctgccgagg
     1081 tgttctcatt ggccgcgcag gtggttcgac gctcgttccc cgaggccgac ctgcccaagg
     1141 ccgtccgctc gaccggggcg cagccgcaac tggtggggac cgaacccgag tcgttcctgc
     1201 gggtggtcgc cgaacaggcg gcccggttgc tcgatcaggt ggccggaacc gtcggcgtga
     1261 tcgccccacc ctcgcggttc gaggcggtgc gcgcgacgct cgaaccgctg gccgacaacc
     1321 ggttggtggt ggtcacccca ctgcaggcca aaggtctcga atacgacggt gtgctggtgg
     1381 ccgcccccga tgacatcgtg gccgagtcgc cgggcggcga acgggtgctc tacgtggcgc
     1441 tcacgcgtcc gacgcagctg ctggtgaccg tcgatccagt cccagccggc gcaggttggc
     1501 gagaatcgct gacgttgccc gtgaccggtt gagagtgaag aactgcagcc ccggggcgcc
     1561 gccgcgcagc acgtcggcgc tcagttcggt ggcgatctcg atgcccactc ggcgcacctg
     1621 agcggggtcg cccgcgaccg cgtgcagccg gcggacaacg cgcgtgggca actcagcgcc
     1681 cgacagtgcc gcgaaccgtt cgatctgggc gatgttggtg accggctgga tgcccgcgac
     1741 gatcggcagc gtgcagccga tcgcgcgggc gcgctcgacc agtcgcaggt aggcgtcggc
     1801 gtcgaagaag agctgggtga ccgcgaagct ggcgcccgcc tcttgcttgg ccagcagcag
     1861 cctggcgtcc aggtcggcat cgtgttgctg cgggtgcggg tcgggaaagg ccgccacccc
     1921 gataccgaag ctgaagttgc cggcgatcag gcgaaccagc tcggtggcgt tggccagacc
     1981 gtcgggatgc cgctgccacg gcaccgtcgg gccaccgggc atgtcgccgc ggatggccag
     2041 cacatgccgc acgtccgctt cggcgtaggc ggccagggtg gccagcagtt cgtcccggga
     2101 ttggctcgcg caggtcaggt ggcccatggt ctgggcctcg gtgcgccgtt tgatgaaccg
     2161 ggtggcgcgg atcgtgcggt cacgggtcgc gccggaggct ccgtaggtga ccgagagcca
     2221 gtcggcaccg gtggcgtcca ggtcgagaat ggtctgggcg agcacgtcct cgcccgccac
     2281 atcggcgggc ggaaagaact cgaagctgaa cgtcggacgg ctggtgtcgg cgagcaggtc
     2341 ggcgatgctg ggcaggtgct cctgcgcggc gacgtcatcg ggggtcacgg acacgggcac
     2401 caaggtaggc cagcgccgtc cggctcgggc ctctaggctc agtggatggg cattttcgcg
     2461 gcgcacgatc cgttgggcgc tccgttccgc gacgcgatgg gtgcgcacct gcgcaccttc
     2521 atcgaggcgc ggcgcgagca gttgaccgcg gtgggcccgc aattgcgggc actgaccgac
     2581 ctcgcgttgg cgttcacggc gggtggcaag cggctccggc cggcgttctg ctggtggggg
     2641 tactgctcga tcgtcgaacc cgacgatgcc gaggcgctgc tgcgggcggc cgcaagcctc
     2701 gacctgctgc acgtgtcggc gctggtgcac gacgacgtga tggactcctc ggacacccgg
     2761 cgcgggctgg cggccgcaca tcggcagttc gagacctggc atcacgaagg tggcctgctg
     2821 ggttcacccg aggcgttcgg acgggcgggc gcgattctgc tcggcgacct gctgctggtg
     2881 tggtcgatgg aggcgttcac cggttcgggg ttgccgatgg ctgcgctggc gcgggcgact
     2941 cccctactgg acgcgatgcg caccgaagtg acgtgcgggc agttcctcga cgtgttcgcc
     3001 caggccgagc cggtcggcgg ggtcgacctg atgagcctgg tgccgcaggt ggtggagttc
     3061 aagacctccc ggtacacggt gatccgcccc ttgcaggtgg gggcggcgct ggcgggcgcg
     3121 tccgagccgg cgttggccgc attggctgcc tacggctccc ccctgggccg cgccttccag
     3181 ttccgcgacg acgtgctcgg ggtgttcggc gagcccgagg tgaccggcaa acccgccggt
     3241 gacgacctgc gcgagggaaa gcgcacggtc ttggtcgcgc acgcgttgga gctggcgtcc
     3301 gagacggcga aggaacgcct gatcgggttg ctgggcagtt ctgcactgga cgcgaccgac
     3361 atcgcgacgg cccgcgagat catcgtgcag tcgggcgcgc tggccgcaac cgagcaacgg
     3421 atcaccgacg acc
//
LOCUS       DAOSUG010000344         3428 bp    DNA     linear   ENV 10-JUN-2024
DEFINITION  MAG TPA_asm: Micropruina sp. isolate AMR_MDS_2195
            NODE_11282_length_3428_cov_3.909576, whole genome shotgun sequence.
ACCESSION   DAOSUG010000344 DAOSUG010000000
VERSION     DAOSUG010000344.1
DBLINK      BioProject: PRJNA925477
            BioSample: SAMN32864262
            Sequence Read Archive: SRR4236673
KEYWORDS    WGS; ENV; Metagenome Assembled Genome; MAG; Third Party Data; TPA;
            TPA:assembly.
SOURCE      Micropruina sp. (wastewater metagenome)
  ORGANISM  Micropruina sp.
            Bacteria; Bacillati; Actinomycetota; Actinomycetes;
            Propionibacteriales; Nocardioidaceae; Micropruina.
REFERENCE   1  (bases 1 to 3428)
  AUTHORS   Abdulkadir,N., Saraiva,J.P., Zhang,J., Stolte,S., Gillor,O.,
            Harms,H. and Rocha,U.
  TITLE     Genome-centric analyses of 165 metagenomes show that mobile genetic
            elements are crucial for the transmission of antimicrobial
            resistance genes to pathogens in activated sludge and wastewater
  JOURNAL   Microbiol Spectr 12 (3), e0291823 (2024)
   PUBMED   38289113
REFERENCE   2  (bases 1 to 3428)
  AUTHORS   Abdulkadir,N.
  TITLE     Direct Submission
  JOURNAL   Submitted (24-MAR-2023) Environmental Microbiology, Helmholtz
            Center for Environmental Research -UFZ, Permoserstr 15, Leipzig
            04318, Germany
COMMENT     The annotation was added by the NCBI Prokaryotic Genome Annotation
            Pipeline (PGAP). Information about PGAP can be found here:
            https://www.ncbi.nlm.nih.gov/genome/annotation_prok/
            
            ##Genome-Assembly-Data-START##
            Assembly Method        :: SPAdes v. 3.11.1
            Genome Representation  :: Full
            Expected Final Version :: Yes
            Genome Coverage        :: Not Applicable
            Sequencing Technology  :: Illumina MiSeq
            ##Genome-Assembly-Data-END##
            
            ##Genome-Annotation-Data-START##
            Annotation Provider               :: NCBI
            Annotation Date                   :: 05/04/2023 04:57:10
            Annotation Pipeline               :: NCBI Prokaryotic Genome
                                                 Annotation Pipeline (PGAP)
            Annotation Method                 :: Best-placed reference protein
                                                 set; GeneMarkS-2+
            Annotation Software revision      :: 6.5
            Features Annotated                :: Gene; CDS; rRNA; tRNA; ncRNA
            Genes (total)                     :: 2,776
            CDSs (total)                      :: 2,750
            Genes (coding)                    :: 2,714
            CDSs (with protein)               :: 2,714
            Genes (RNA)                       :: 26
            tRNAs                             :: 24
            ncRNAs                            :: 2
            Pseudo Genes (total)              :: 36
            CDSs (without protein)            :: 36
            Pseudo Genes (ambiguous residues) :: 0 of 36
            Pseudo Genes (frameshifted)       :: 2 of 36
            Pseudo Genes (incomplete)         :: 32 of 36
            Pseudo Genes (internal stop)      :: 2 of 36
            ##Genome-Annotation-Data-END##
FEATURES             Location/Qualifiers
     source          1..3428
                     /organism="Micropruina sp."
                     /mol_type="genomic DNA"
                     /submitter_seqid="NODE_11282_length_3428_cov_3.909576"
                     /isolate="AMR_MDS_2195"
                     /isolation_source="Activated sludge of municipal
                     wastewater treatment plants"
                     /db_xref="taxon:2737536"
                     /environmental_sample
                     /geo_loc_name="Canada"
                     /lat_lon="49.25 N 123.1 W"
                     /collection_date="2015-06-16"
                     /metagenome_source="wastewater metagenome"
                     /note="metagenomic"
     gene            complement(<1..1065)
                     /locus_tag="PLF56_10740"
     CDS             complement(<1..1065)
                     /locus_tag="PLF56_10740"
                     /inference="COORDINATES: protein motif:HMM:NF012382.2"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_function: GO:0030170 - pyridoxal phosphate binding
                     [Evidence IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="PLP-dependent aminotransferase family protein"
                     /protein_id="HOQ54075.1"
                     /translation="MSSTVSAGRLATLVGTVPSPAFAGLASALARLIADGRLPPGLRL
                     PSERELAKALTLSRTTITRAYSTLREAGYARARQGSGTWTHVPGGTARGRDRALLPQP
                     QRASVMDLTCAAPASTPGLASAYAAAVDELPAYLTGHGYFPLGLPELRAALAAQYDRR
                     GLATDPEQILVTSGAIAAIAAAAQALVGPGDRAMVESPVYPNAVQSLRRAGARVVSTP
                     VDPHAWDLDALASTLRQTSPRVAYLIPDYQNPTGLLMPDDERQRVASALAATRTVALV
                     DESHAALRLDDLHPTDAQLTGAVDDPPFARASEPLPFAFHAPSTISIGGASKTYWGGL
                     RVGWLRVPTALVPRLIAARLG"
     gene            1128..1805
                     /locus_tag="PLF56_10745"
     CDS             1128..1805
                     /locus_tag="PLF56_10745"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_013863412.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="hypothetical protein"
                     /protein_id="HOQ54076.1"
                     /translation="MSARTYEISNLGPIGQLRTGRLPRRLVQLMAGLALFGFSIALMV
                     RGALGLAPWDVLHSGLVRWLPLTLGQAIVLGSLAVLLLWIPLGERPGIGTLANAVVIG
                     LVADASLHLLPVLDGWPSRLLVMVAGVVLNGLASAVYIGAQLGRGPRDGLMTGLARRT
                     GRSLRLVRTLLEVVVLAFGLVLGGSAGLGTALFALAIGPLTQFFLPWVTVREVGQAVP
                     DEGSSAG"
     gene            complement(2415..2696)
                     /locus_tag="PLF56_10750"
     CDS             complement(2415..2696)
                     /locus_tag="PLF56_10750"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_017934145.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="hypothetical protein"
                     /protein_id="HOQ54077.1"
                     /translation="MWLAALDDGWRVLLAGLLLGSGLPILFALGVKSIAWANGDLPGR
                     AANPAGKVVAGLLFAVVLVAVMLGIGYIVAHGFGYTLGWNGWLPSFTHK"
     gene            complement(2696..>3428)
                     /locus_tag="PLF56_10755"
     CDS             complement(2696..>3428)
                     /locus_tag="PLF56_10755"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_013863866.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_component: GO:0016020 - membrane [Evidence IEA];
                     GO_process: GO:0006817 - phosphate ion transport [Evidence
                     IEA];
                     GO_process: GO:0055085 - transmembrane transport [Evidence
                     IEA]"
                     /codon_start=2
                     /transl_table=11
                     /product="inorganic phosphate transporter"
                     /protein_id="HOQ54078.1"
                     /translation="AIAMIVAMVGTHFVFKVAANLTEKYRTQGFRWGQVGAASLMSLS
                     HGTNDAQKTMGVITLALIASGEWGDTHAVPLWVKLACGFTIAMGTYLGGWRIIRTMGK
                     GLVEIEAPQGMSADMSSAAVILASSQLGFALSTTHVATGSILGSGVGRPGAQVRWGVA
                     GRMASAWLTTLPSAAAVSALMFVIGHALGAGWGSAVIVALLVAAGLYMYLHSRKEPIG
                     HHNVNDEWDDAKKPKKKNDKREVAV"
BASE COUNT          489 a         1182 c         1227 g          530 t
ORIGIN      
        1 tcccagccgc gccgcgatca ggcgcggcac cagggcagtc ggcacccgca gccagcccac
       61 ccgcagcccg ccccagtacg tcttggacgc acctccgatg ctgatcgtgg acggcgcgtg
      121 gaacgcgaac ggcaacggct ccgacgcccg ggcgaacggc ggatcgtcca cggcaccggt
      181 cagctgagcg tccgtcgggt gcaggtcgtc cagccgcagg gcggcgtgac tctcgtccac
      241 cagggcgacc gtgcgggtcg cggccagcgc cgaggcgacc cgctgccgct catcgtccgg
      301 catcaacagc ccggtcgggt tctggtagtc cggaatcagg taggccaccc ggggggacgt
      361 ctgccgcagg gtgctggcca gcgcgtccag atcccaggcg tgggggtcga ccggcgtgct
      421 caccactcgt gccccggcgc gccgcagcga ttgcaccgcg ttggggtaga ccggcgattc
      481 caccatggcc cgatccccag ggccgaccag ggcctgggcg gcggccgcga tggcggcgat
      541 cgcgcccgag gtgacgagaa tctgctcggg atcggtcgcg aggccacgac ggtcgtactg
      601 ggccgcgagc gccgcgcgca gctcgggcag gccgagggga aagtagccgt gcccggtcag
      661 gtacgcgggc agttcgtcaa cggcggcggc gtaggcgctg gccagccccg gggtggacgc
      721 cggcgcggcg caggtcagat ccatcaccga ggcgcgctgg ggctggggca acagtgcgcg
      781 gtcgcggccc cgggcggtgc cgccgggcac gtgcgtccag gttccggaac cctgccgcgc
      841 gcgtgcgtag ccggcctcgc gcagcgtgga gtacgcccgc gtgatcgtgg tgcgcgacaa
      901 ggtgagcgcc ttggccagct cgcgctcgct gggcaggcga aggcctggcg ggaggcgtcc
      961 gtcggcgatc aggcgggcca acgccgacgc caggccggcg aacgcgggcg aggggacagt
     1021 ccctaccaaa gtggccagcc ggccagcgga taccgtgctg ctcaccaggc cactcttgca
     1081 tgattggctc tttgcgacaa ggccaatcag atgccagggt ggacaccatg agcgcgcgaa
     1141 cctatgagat ctcgaatctc ggccccatcg gccaactgcg caccgggcgg ttgccgcgcc
     1201 gcctggtgca gctgatggcc gggctggccc tgttcgggtt ctcgatcgca ctgatggtgc
     1261 gcggcgcgct cggtctcgcg ccgtgggacg tgctgcactc gggcctggtg cgctggctgc
     1321 cgctcaccct tggccaggcc atcgtgctgg gcagtctcgc ggtgttgctg ctgtggattc
     1381 cactgggcga gcgacccggc atcggcacgc tggccaacgc cgtcgtgatc ggcctggtcg
     1441 ccgacgcctc gttgcacctg ctgccggtgc tggacgggtg gccgtcccga ctgttggtga
     1501 tggtcgccgg ggtggtgttg aacgggctgg cgagtgcggt ctacatcggt gcgcagttgg
     1561 gccgtgggcc gcgcgacggg ttgatgaccg gcctggcccg gcgcaccgga cgaagcctgc
     1621 ggctcgtccg caccctgctc gaggtggtgg tattggcctt cgggctggtg ctgggcggat
     1681 cggccgggct cggcacggcg ctgttcgccc ttgccatcgg ccccttgacg cagttctttc
     1741 tgccctgggt cacggtgcgc gaggtcgggc aggcagtgcc ggacgagggt tcgtccgcgg
     1801 ggtgaccggt gggcttggtc aggcgtgtcg cgccgctggt cgaggccctg tcggttggct
     1861 tgtcgcacac acacactggt gggcctcgct ggtcgagctt gtcggggcac tgtccggcga
     1921 ttcgtgcccg tgcgccaatc tgcccgccat cggttgatga acaccaagtc cgccactgga
     1981 ttcgtggatg ggtcgcaatt gaatcgtgca agttgcaatc gagtgctgga ttcagtactg
     2041 cggtccgggt ctgatcgaag accaatcacc gtgcgagtgg cggcaggcgc cgtcggggtc
     2101 gccggcatcg tgcgtaaggc aaatggcggg ggagtgatcc cgtccccacg ccgttgagcc
     2161 cccctctggc tgacgttgcg cggtgaagcg ctcgttgcgc ttggacggac ttgcgcaatg
     2221 agccctagac cgcgcaacct cgaccccgcc cggcggaccg ggggcctcga cagggtcgag
     2281 cagacccggg ttccgacagc gagcggatcg gggtctcgga acccggcccg gcggacgggc
     2341 tctcgacagg ctggccctgt ggccctgtgg ccctgtggcc ctgtggccgg ggtctcgaca
     2401 ggcttgacca gcgactactt gtgggtgaag ctgggcaacc agccgttcca acccagggtg
     2461 tacccgaagc cgtgggcgac gatgtagccg atgcccagca tcactgccac cagcaccacc
     2521 gcgaacagca gcccggcgac caccttgccg gccggattgg cggcgcgacc cggtaggtca
     2581 ccgttggccc acgcgatgga tttgaccccg agtgcgaaca ggatcggcag gcccgaaccc
     2641 agcagcaggc cagcgagcag gacccgccag ccgtcgtcca gcgcggcgag ccacatcaga
     2701 ccgccacctc acgcttgtcg ttcttcttct tgggcttctt ggcgtcgtcc cactcgtcgt
     2761 tgacgttgtg gtggccgatc ggctccttgc gcgaatgcag gtacatgtag agcccggcgg
     2821 cgaccagcag ggccacgatg actgcggacc cccagccggc gcccagcgcg tgcccgatga
     2881 cgaacatcag ggcgctcacc gcggccgcgg acggcagcgt ggtcaaccag gccgaggcca
     2941 tccgtccggc gactccccag cgcacctgcg cccccggacg tcccacgccc gagcccagga
     3001 tcgaccccgt ggccacgtgg gtggtcgaga gcgcgaaccc cagctggctg gaggccagga
     3061 tcaccgccgc cgagctcatg tcggcgctca tgccctgcgg ggcctcgatc tcgaccagcc
     3121 ccttgcccat ggtgcgaatg atgcgccacc cacccagata ggtgcccatc gcgatcgtga
     3181 aaccgcacgc cagcttgacc cacaggggca cggcgtgggt gtcgccccac tcacccgagg
     3241 cgatcagtgc cagcgtgatc acgcccatcg tcttctgcgc gtcgttggtg ccgtgcgaca
     3301 acgacatcag cgaggccgcg ccgacctgtc cccaacggaa cccctgcgtg cggtacttct
     3361 cggtgaggtt cgccgccacc ttgaacacga agtgcgtgcc gaccatggcc acgatcatcg
     3421 cgatcgcc
//
LOCUS       DAOSUG010000345         3424 bp    DNA     linear   ENV 10-JUN-2024
DEFINITION  MAG TPA_asm: Micropruina sp. isolate AMR_MDS_2195
            NODE_11299_length_3424_cov_4.129712, whole genome shotgun sequence.
ACCESSION   DAOSUG010000345 DAOSUG010000000
VERSION     DAOSUG010000345.1
DBLINK      BioProject: PRJNA925477
            BioSample: SAMN32864262
            Sequence Read Archive: SRR4236673
KEYWORDS    WGS; ENV; Metagenome Assembled Genome; MAG; Third Party Data; TPA;
            TPA:assembly.
SOURCE      Micropruina sp. (wastewater metagenome)
  ORGANISM  Micropruina sp.
            Bacteria; Bacillati; Actinomycetota; Actinomycetes;
            Propionibacteriales; Nocardioidaceae; Micropruina.
REFERENCE   1  (bases 1 to 3424)
  AUTHORS   Abdulkadir,N., Saraiva,J.P., Zhang,J., Stolte,S., Gillor,O.,
            Harms,H. and Rocha,U.
  TITLE     Genome-centric analyses of 165 metagenomes show that mobile genetic
            elements are crucial for the transmission of antimicrobial
            resistance genes to pathogens in activated sludge and wastewater
  JOURNAL   Microbiol Spectr 12 (3), e0291823 (2024)
   PUBMED   38289113
REFERENCE   2  (bases 1 to 3424)
  AUTHORS   Abdulkadir,N.
  TITLE     Direct Submission
  JOURNAL   Submitted (24-MAR-2023) Environmental Microbiology, Helmholtz
            Center for Environmental Research -UFZ, Permoserstr 15, Leipzig
            04318, Germany
COMMENT     The annotation was added by the NCBI Prokaryotic Genome Annotation
            Pipeline (PGAP). Information about PGAP can be found here:
            https://www.ncbi.nlm.nih.gov/genome/annotation_prok/
            
            ##Genome-Assembly-Data-START##
            Assembly Method        :: SPAdes v. 3.11.1
            Genome Representation  :: Full
            Expected Final Version :: Yes
            Genome Coverage        :: Not Applicable
            Sequencing Technology  :: Illumina MiSeq
            ##Genome-Assembly-Data-END##
            
            ##Genome-Annotation-Data-START##
            Annotation Provider               :: NCBI
            Annotation Date                   :: 05/04/2023 04:57:10
            Annotation Pipeline               :: NCBI Prokaryotic Genome
                                                 Annotation Pipeline (PGAP)
            Annotation Method                 :: Best-placed reference protein
                                                 set; GeneMarkS-2+
            Annotation Software revision      :: 6.5
            Features Annotated                :: Gene; CDS; rRNA; tRNA; ncRNA
            Genes (total)                     :: 2,776
            CDSs (total)                      :: 2,750
            Genes (coding)                    :: 2,714
            CDSs (with protein)               :: 2,714
            Genes (RNA)                       :: 26
            tRNAs                             :: 24
            ncRNAs                            :: 2
            Pseudo Genes (total)              :: 36
            CDSs (without protein)            :: 36
            Pseudo Genes (ambiguous residues) :: 0 of 36
            Pseudo Genes (frameshifted)       :: 2 of 36
            Pseudo Genes (incomplete)         :: 32 of 36
            Pseudo Genes (internal stop)      :: 2 of 36
            ##Genome-Annotation-Data-END##
FEATURES             Location/Qualifiers
     source          1..3424
                     /organism="Micropruina sp."
                     /mol_type="genomic DNA"
                     /submitter_seqid="NODE_11299_length_3424_cov_4.129712"
                     /isolate="AMR_MDS_2195"
                     /isolation_source="Activated sludge of municipal
                     wastewater treatment plants"
                     /db_xref="taxon:2737536"
                     /environmental_sample
                     /geo_loc_name="Canada"
                     /lat_lon="49.25 N 123.1 W"
                     /collection_date="2015-06-16"
                     /metagenome_source="wastewater metagenome"
                     /note="metagenomic"
     gene            438..3017
                     /locus_tag="PLF56_10760"
     CDS             438..3017
                     /locus_tag="PLF56_10760"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_016668185.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="sucrose phosphorylase"
                     /protein_id="HOQ54079.1"
                     /translation="MPLFPALTDPNSIPTGDMPGDKVHITDWHLAAAATLYPALREQL
                     DPVLAAGRAVVAVYGGSGVGKSELGSLLGHALNADGIGAYILSGDNYPRRIPSVNDAE
                     RLRVFRHAGVRGLVDAGAYDASVRDALSSLQAAGTDADPAAAAEHPWLRTYLGAGSTA
                     LAAYLGTPNEIDFAEVNAIIAAFKGGADTLTLKRMGRTEADQWYDQVDVSGTRVLVIE
                     WTHGNSDFVEGVDVPILLNSTPAQTLAHRRARARDGAVDSPFTTLVLGLEQAKLDAQA
                     PKASLILSKEGELLTYDAYLQAMGRHLPRDGVMLNAYPDSLDGTLAGLAAFVQRPEVA
                     GAFTSLYLLPSVFNSDLDRGFSVIDYGLNDLLAKPADLEALAAAGLDLKFDFILNHAS
                     VLSPQFQDILARGERSPYNDFFIDWNAFWAGHGEMTPEGYIQPRADLIEHMFFRKPGL
                     PLLMVRLPDGSEKPFWNTFYQEVRYPRVDAQDLMAAGLQYASADELATRVNATIADGG
                     RPGDADFTGFEAWRDAVVDLVESRRTYLGQMDLNIKSHLVWTFYADTLARLAGYGAEI
                     VRLDAFAYAPKEPGEKNFLNDPGTWDLLDQVKALADRHGVKLLPEIHARYEEGIHETI
                     AAKGFLTYDFFLPGLLIHALETHSAERLLAWIGDIQAKGIKTVSMLGCHDGIPLLDLK
                     GLLSDDEIESVIATVRGRGGYVKDLHGQKATYYQVNATYYSALGCDDASMLLARAIHL
                     FMPGKPQVWYLDLFAGENNLAAVERAGSGGHKEINRTNLSADDLEAGLARPVVQRQID
                     LLRFRNTHPAFGWDAELTASGEGSVLTLEWSREGHTARLEADLATRAFRITADGTDLD
                     SQG"
     gene            complement(3068..>3424)
                     /locus_tag="PLF56_10765"
     CDS             complement(3068..>3424)
                     /locus_tag="PLF56_10765"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_014845773.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_function: GO:0032559 - adenyl ribonucleotide binding
                     [Evidence IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="universal stress protein"
                     /protein_id="HOQ54080.1"
                     /translation="EGAKATNTLGDTRTSVVLGREAASNALATATSTASGLGAQVKAS
                     LLVEGEAASALLRAAQQHEADLIVIGAIRDTSLAGRLLGTVASAVVRAAPCEVLVVRP
                     PAAWGDVTEIEVPEDV"
BASE COUNT          544 a         1228 c         1131 g          521 t
ORIGIN      
        1 gctcgccgat ctcatagctc tcgtccgcct tcagccgata ctcggccaag cgatcctcgt
       61 aggggaagac ggcgacggtc ttgatgccga gctcggagca ggttcccctt gactgcccgc
      121 ggcgatggtc ggcgccgcca gacgacgatg tgccggtgcc cgaggtggaa ccacaactcg
      181 tggattctgt gcgacgtagc gcccgttgga ctaccaatct ccgggtcgca tcgcggggtg
      241 tgagacgaaa ggcgttgtca gaggctttgg ggtgccgacg ggcgatctcc ttggtagtcc
      301 acatggcgct acgcatcgcc cgtgaaccct cgacaatgcc cgtgtgggaa cgcaagccgt
      361 tcgtacgggt cctgtaccca ccgggacacc tccggtggga taatgggcgc ggttccccag
      421 acgacgaagg agtcctcgtg cccttgttcc ccgcgctcac cgatcccaac tcgatcccca
      481 ccggggacat gccgggggac aaggtgcaca tcaccgattg gcatctggcg gctgcggcca
      541 cgctgtaccc cgccctgcgc gagcagctcg accccgtcct ggccgcggga cgcgccgtgg
      601 tggccgtcta cggcggctcg ggcgtgggca aatccgagct gggatcgctg ctcggccacg
      661 cgctcaacgc ggacggcatc ggcgcctaca tcctgtcggg cgacaactac ccgcgccgga
      721 tcccctccgt caacgacgcc gaacgcctgc gcgtcttccg ccacgcgggc gtccgcgggc
      781 tggtggacgc cggggcctac gacgcgtccg tccgggacgc cctctcctcc ctccaggctg
      841 cggggacgga cgccgacccg gcggccgcag ccgagcaccc ttggctccgg acctacctcg
      901 gcgcgggctc cacggcgctc gccgcgtacc tcgggacacc gaacgagatc gacttcgccg
      961 aggtgaacgc gatcatcgcc gccttcaagg gcggcgccga caccctgacc ctcaagcgca
     1021 tggggcgcac cgaggccgac cagtggtatg accaggtcga cgtctcgggc acccgcgtgc
     1081 tcgtcatcga gtggacccac ggcaacagcg acttcgtcga gggcgtggac gtgcccatcc
     1141 tgctcaacag caccccggcg cagacgctgg cgcaccgccg cgctcgcgcc cgtgacgggg
     1201 cggtcgacag tcccttcacg acgctcgtct tgggattgga gcaggccaag ctggacgcgc
     1261 aggcacccaa ggcgtccttg atcctgtcga aagagggcga actgctcacc tacgacgcct
     1321 acctgcaggc gatggggcgc cacctccccc gcgatggcgt catgctgaac gcctaccccg
     1381 acagcctcga cggcaccctg gcgggcctgg cggcgttcgt gcagcgtccc gaggtcgccg
     1441 gcgcgttcac gtcgctgtac ctgctcccca gcgtgttcaa cagcgacctc gaccgcggct
     1501 tcagcgtgat cgactacggg ctgaacgacc ttctggcgaa gcccgccgac ctcgaggcgc
     1561 tggccgctgc ggggctcgac ctgaagttcg acttcatcct caaccacgcc tcggtgctgt
     1621 cgccccagtt ccaagacatc ctggcccgcg gcgagaggtc gccctacaac gacttcttca
     1681 tcgactggaa cgccttctgg gccggtcacg gcgagatgac gccggagggc tacatccagc
     1741 cacgcgcgga cctgatcgag cacatgttct tccgcaagcc gggcctgccg ctgttgatgg
     1801 tgcgtctgcc cgacgggtcg gagaagccgt tctggaacac cttctaccag gaggtccgct
     1861 acccccgcgt ggacgcccag gacctgatgg cggccggcct gcagtacgcc tcggcggacg
     1921 agttggccac ccgggtcaac gccaccatcg cggacggcgg acgacccggg gatgctgact
     1981 tcaccggctt cgaggcgtgg cgggacgccg tggtcgacct ggtggagtcg cggcgcacct
     2041 acctgggaca gatggacctc aacatcaagt cgcacctggt gtggacgttc tacgccgaca
     2101 ccctggccag gctcgcgggc tacggcgccg agatcgtccg gctggacgct ttcgcctacg
     2161 cccccaagga gccaggcgag aagaacttcc tgaacgaccc cggtacgtgg gatctgctcg
     2221 accaggtgaa ggcgttggcc gaccggcacg gggtgaagct gctgcccgag atccacgccc
     2281 gctacgagga gggcatccac gagacgattg cggccaaggg gttcctcacc tacgacttct
     2341 tcctgcccgg gctgctgatc cacgcgctcg agacgcactc cgccgagcgc ctgctcgcgt
     2401 ggatcggcga catccaggcc aagggcatca agacggtgtc gatgctgggc tgccacgacg
     2461 gcatcccgct gctcgacctc aagggcctgc tcagcgacga cgagatcgag tcggtgatcg
     2521 ccacggtgcg cggacgcggc ggctacgtga aggatctcca cggccagaag gcgacctact
     2581 atcaggtcaa cgccacgtac tacagcgctc tgggctgcga cgacgcatcg atgctgctgg
     2641 cccgggcgat ccacctgttc atgccgggta agccgcaggt gtggtacctc gacctcttcg
     2701 ccggcgagaa caacctggcc gccgtcgagc gtgcggggtc gggcgggcac aaggagatca
     2761 accgcaccaa cctgtcggcc gacgacctcg aagccggcct cgcgaggccc gttgtgcagc
     2821 gccagatcga cttgctgcgg ttccgcaaca cccacccggc gttcgggtgg gatgccgagc
     2881 tgacggcgtc cggagaaggc tcggtgttga ccttggagtg gtcgcgcgag ggccacaccg
     2941 cacggttgga ggccgacctc gcgacccggg cgttccgcat caccgcggac ggcacggacc
     3001 tcgacagcca aggctgaccc tgcgttcggc gcccccggcc ggcgggcagg gtgcgccgtc
     3061 gcagcgctca gacgtcctcg ggcacctcga tctcggtcac gtcaccccac gccgccgggg
     3121 gacgcaccac caggacctcg cacggggctg cacgcacgac ggcggacgcg accgtgccga
     3181 gcaggcgtcc ggcgagactg gtgtctcgga tcgccccgat cacgatgagg tccgcctcgt
     3241 gctgctgcgc ggccctcagg agggcggagg cagcctcgcc ctccaccagc agcgacgcct
     3301 tgacctgcgc gcccaggccg gacgccgtgg atgtcgcggt cgccaacgcg ttgctggccg
     3361 cttcgcgtcc caacacaacg gaggtccggg tgtcaccgag ggtgttcgtc gccttcgcac
     3421 cctc
//
LOCUS       DAOSUG010000346         3414 bp    DNA     linear   ENV 10-JUN-2024
DEFINITION  MAG TPA_asm: Micropruina sp. isolate AMR_MDS_2195
            NODE_11325_length_3414_cov_3.088717, whole genome shotgun sequence.
ACCESSION   DAOSUG010000346 DAOSUG010000000
VERSION     DAOSUG010000346.1
DBLINK      BioProject: PRJNA925477
            BioSample: SAMN32864262
            Sequence Read Archive: SRR4236673
KEYWORDS    WGS; ENV; Metagenome Assembled Genome; MAG; Third Party Data; TPA;
            TPA:assembly.
SOURCE      Micropruina sp. (wastewater metagenome)
  ORGANISM  Micropruina sp.
            Bacteria; Bacillati; Actinomycetota; Actinomycetes;
            Propionibacteriales; Nocardioidaceae; Micropruina.
REFERENCE   1  (bases 1 to 3414)
  AUTHORS   Abdulkadir,N., Saraiva,J.P., Zhang,J., Stolte,S., Gillor,O.,
            Harms,H. and Rocha,U.
  TITLE     Genome-centric analyses of 165 metagenomes show that mobile genetic
            elements are crucial for the transmission of antimicrobial
            resistance genes to pathogens in activated sludge and wastewater
  JOURNAL   Microbiol Spectr 12 (3), e0291823 (2024)
   PUBMED   38289113
REFERENCE   2  (bases 1 to 3414)
  AUTHORS   Abdulkadir,N.
  TITLE     Direct Submission
  JOURNAL   Submitted (24-MAR-2023) Environmental Microbiology, Helmholtz
            Center for Environmental Research -UFZ, Permoserstr 15, Leipzig
            04318, Germany
COMMENT     The annotation was added by the NCBI Prokaryotic Genome Annotation
            Pipeline (PGAP). Information about PGAP can be found here:
            https://www.ncbi.nlm.nih.gov/genome/annotation_prok/
            
            ##Genome-Assembly-Data-START##
            Assembly Method        :: SPAdes v. 3.11.1
            Genome Representation  :: Full
            Expected Final Version :: Yes
            Genome Coverage        :: Not Applicable
            Sequencing Technology  :: Illumina MiSeq
            ##Genome-Assembly-Data-END##
            
            ##Genome-Annotation-Data-START##
            Annotation Provider               :: NCBI
            Annotation Date                   :: 05/04/2023 04:57:10
            Annotation Pipeline               :: NCBI Prokaryotic Genome
                                                 Annotation Pipeline (PGAP)
            Annotation Method                 :: Best-placed reference protein
                                                 set; GeneMarkS-2+
            Annotation Software revision      :: 6.5
            Features Annotated                :: Gene; CDS; rRNA; tRNA; ncRNA
            Genes (total)                     :: 2,776
            CDSs (total)                      :: 2,750
            Genes (coding)                    :: 2,714
            CDSs (with protein)               :: 2,714
            Genes (RNA)                       :: 26
            tRNAs                             :: 24
            ncRNAs                            :: 2
            Pseudo Genes (total)              :: 36
            CDSs (without protein)            :: 36
            Pseudo Genes (ambiguous residues) :: 0 of 36
            Pseudo Genes (frameshifted)       :: 2 of 36
            Pseudo Genes (incomplete)         :: 32 of 36
            Pseudo Genes (internal stop)      :: 2 of 36
            ##Genome-Annotation-Data-END##
FEATURES             Location/Qualifiers
     source          1..3414
                     /organism="Micropruina sp."
                     /mol_type="genomic DNA"
                     /submitter_seqid="NODE_11325_length_3414_cov_3.088717"
                     /isolate="AMR_MDS_2195"
                     /isolation_source="Activated sludge of municipal
                     wastewater treatment plants"
                     /db_xref="taxon:2737536"
                     /environmental_sample
                     /geo_loc_name="Canada"
                     /lat_lon="49.25 N 123.1 W"
                     /collection_date="2015-06-16"
                     /metagenome_source="wastewater metagenome"
                     /note="metagenomic"
     gene            complement(<1..613)
                     /locus_tag="PLF56_10770"
     CDS             complement(<1..613)
                     /locus_tag="PLF56_10770"
                     /inference="COORDINATES: protein motif:HMM:NF020877.2"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="alpha-glucosyltransferase N-terminal
                     domain-containing protein"
                     /protein_id="HOQ54081.1"
                     /translation="MDLKKRLRRALAAAGLLGVVRRLRKGRATTHQPKPRRATALPPA
                     PALQPFPQARYIFATGAIPRRYAGRSASVLAKATLFSERAGVPCEILTMNFSSELDDV
                     THEIDQRGALGEGVRITNLYDSLWQEGEPREPVQHPIDQPGMDWIKDPDGAVYRYYEN
                     GVYRVYRRFDYAGRLIVQDWFNENRGRTQRDEFGPDGHIRRTTY"
     gene            complement(613..2202)
                     /locus_tag="PLF56_10775"
     CDS             complement(613..2202)
                     /locus_tag="PLF56_10775"
                     /EC_number="2.4.-.-"
                     /inference="COORDINATES: protein motif:HMM:NF012744.2"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="glycosyltransferase"
                     /protein_id="HOQ54082.1"
                     /translation="MTDAVRLPDAEFVFLVDVLPRNHAGRSASILTKTRLLWQHCGVR
                     STIVTAYDSSQLDDIGHGLRERGLLAEGVTAASLHDYFPDPSTPPEGDFNRPFDEPGM
                     AWVEDPEAGQFHFYEHGRHRLSKRVDYAARTIVLDWLDGARNRTRSDEYWPNGIQRRT
                     VFFDLHYGVARQELLHRRDGTVRLGIWWAVDPVTKVRSPQRVTEFDEQGRPARVLDSY
                     YDVIHASLDGLVGERPAFVVCEARRIDEWVLGYQRPNVRQIHVLHNAHLRPPYDDVHR
                     IRPIYAPLLTSPAVDATVFLTHRQRAEAEAHFGPHDSYHVIPHSVPDAAPGEPVPRDP
                     ALVVMMARLDQQKQVDHAIAAFAEVVKALPEARLDIYGRGVLEGRLNQQIRSLGLSAN
                     VRLAGFTGDPMAVYRGAALSLLTSRYEGFGLVLVESLQQGCPVVSYDLAYGPADIVQD
                     GVNGFLIPPGDRAALAGAIVRVLTEPGLLAALSAAAPGSVKEFSEAAFVQRWAALFGS
                     LASTFPDSQAEPIAAAVQERN"
     gene            2320..>3414
                     /locus_tag="PLF56_10780"
     CDS             2320..>3414
                     /locus_tag="PLF56_10780"
                     /inference="COORDINATES: protein motif:HMM:NF013621.2"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_function: GO:0016491 - oxidoreductase activity
                     [Evidence IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="Gfo/Idh/MocA family oxidoreductase"
                     /protein_id="HOQ54083.1"
                     /translation="MTRVITYGTFDLFHEGHRRLLERAKELGDHLIVAVTTDNYDDSR
                     GKLNVQQSLMERIHNVQESGLADEIIIEEYEGQKVQDIQKYEVDIFAIGSDWLGKFDY
                     LNAYCKVVYLDRTKGVSSTQLRNESVGVLRVGIVGAGRIAARFVEEARYVSGVSVEAV
                     YSRSPERTRAFADRCELQWANDSFDDLLQHVDAIYIASPHATHHDYAVRAIDAGVHVL
                     CEKPMTLSKNETRQLYERAAARRVVLVEAIKTAFSPGFQRMVAVARIGTIGTVRSVDA
                     TFTKLVDEGRELHGTDGGSISELATYPLLAIVKLLGTDFDEVRTYSYRPEGAEVELFS
                     RIDVTYPHAIASARTGIGVKAEGDLVVAGTK"
BASE COUNT          542 a         1138 c         1201 g          533 t
ORIGIN      
        1 agtaggtggt acggcggatg tgcccgtccg ggccgaactc gtcgcgctgg gtgcggcccc
       61 ggttctcatt gaaccagtcc tggacgatca gccggccggc gtagtcgaag cgtcggtaga
      121 cccggtacac gccgttctcg tagtagcggt agacggctcc gtcggggtcc ttgatccagt
      181 ccatgccggg ctggtcgatc gggtgctgga cgggttcgcg aggctcgccc tcctgccaca
      241 gcgagtcgta cagattggtg atccggacgc cctcgcccag cgccccccgc tggtcgatct
      301 cgtgggtgac gtcgtcgagt tcggacgaga agttcatggt caggatctcg cagggcaccc
      361 cggcccgctc cgagaacagc gtggccttgg ccagcaccga ggcgctgcga cccgcataac
      421 ggcgcgggat ggcaccggtc gcgaagatgt agcgtgcctg cgggaacggc tggagggcag
      481 gtgccggggg cagcgccgtc gcacgcctgg gcttcggctg gtgggtggtt gcccggccct
      541 tgcgtaaccg gcggacgaca ccgagcaggc cggccgcggc cagcgcgcgc cggagcctct
      601 tcttcagatc catcagttcc tctcctggac cgctgctgcg atcggttctg cctgcgaatc
      661 ggggaaggtg ctcgccaacg acccgaacaa tgccgcccag cgttgcacga aggcggcctc
      721 gctgaactct ttcaccgacc ccggagcggc cgcggacaag gccgccagca gcccgggctc
      781 cgtcaacacc cggacgatgg cgccggccag cgctgcgcgg tcgccgggtg gaatgaggaa
      841 gccgttgacg ccgtcctgga cgatgtcggc cggtccgtac gcgaggtcgt agctgaccac
      901 cgggcagccc tgctgcagac tttcgaccag caccagcccg aagccctcgt agcggctggt
      961 gagcagcgac agggcggccc cgcgatagac cgccatcggg tcgccggtga aacccgccag
     1021 gcgaacgttt gcgctgagcc cgaggctgcg gatctgctgg ttgagcctgc cctcgagcac
     1081 gccgcgtccg tagatgtcca ggcgggcctc cggtagggcc ttcaccacct cggcgaaggc
     1141 ggcgatcgcg tggtcgacct gcttctgctg gtcgagccgc gccatcatca ccaccagtgc
     1201 gggatcgcgc ggcacgggct cgccgggggc ggcgtcgggc accgagtggg ggatcacgtg
     1261 gtagctgtca tgcggcccga agtgggcttc ggcttcggca cgttggcggt gggtcaggaa
     1321 cacggtggcg tccaccgccg gactggtcag cagcggggcg tagatgggcc tgatccggtg
     1381 cacgtcgtcg tagggtggcc gcagatgcgc gttgtgcagc acatggatct gccgcacgtt
     1441 gggccgctgg tacccgagca cccattcgtc gatccgccgg gcctcgcaca ccacgaaggc
     1501 cggacgctcg ccgaccagcc cgtccaggct ggcgtggatc acgtcgtagt aggagtcgag
     1561 cacgcgggcc ggccggccct gctcgtcgaa ctcggtcacc cgctgcgggc tgcgcacctt
     1621 ggtgaccgga tcgaccgccc accagatgcc cagccgtacg gtgccgtcgc gccggtgcag
     1681 caactcctgc cgggccacgc cgtagtggag atcgaagaac accgtgcgcc gctggatgcc
     1741 gttgggccag tactcgtcgc tgcgggtgcg gttgcgggcc ccgtccaacc agtccagcac
     1801 gatcgtgcgt gcggcgtagt cgacgcgttt ggagaggcgg tgccggccgt gctcgtagaa
     1861 atggaactgt ccggcctcgg ggtcctcgac ccaggccatg cccggctcgt cgaacggacg
     1921 gttgaaatcg ccctcgggtg gcgtggacgg gtcgggaaag tagtcgtgca ggctggcggc
     1981 cgtgacgccc tcggcaagca aaccccgttc acgcaggccg tggccgatgt cgtccagttg
     2041 ggaggagtcg taggcggtca cgatggtgga gcgcaccccg cagtgctgcc acagcagccg
     2101 ggtcttggtc aggatcgacg cgctgcggcc ggcgtggttg cgggggagca cgtcgaccag
     2161 gaacacgaac tcagcgtcgg gcaacctcac cgcgtcggtc atcccaaccc cctatcctgg
     2221 ccacatgtta gcgacgccgc cggctcttct ccgctcgcca caacgggtac cacaggtggc
     2281 gggccgggcg gcccacaggc gcgccctgga ggccggccgg tgacccgcgt catcacctat
     2341 ggcaccttcg acctgttcca cgagggccac cgccggctgc tcgagcgggc caaagagttg
     2401 ggtgaccacc tgatcgtggc ggtcaccacc gacaactacg acgactcgcg cggcaagctg
     2461 aacgtgcagc agagcctgat ggagcgcatc cacaacgtgc aggagtccgg tctcgccgac
     2521 gagatcatca tcgaagagta cgagggccag aaggtccagg acatacagaa gtacgaggtc
     2581 gacatcttcg ccatcggcag cgactggctg ggcaagttcg actacctcaa cgcctactgc
     2641 aaggtggtct acctcgaccg caccaagggt gtgtccagta ctcagttgcg caacgagtcg
     2701 gtcggcgtgc tgcgggtcgg catcgtgggc gccggacgca tcgcggcccg tttcgtcgaa
     2761 gaggcccggt acgtcagcgg ggtcagcgtc gaggccgtct acagccgcag cccggagcgc
     2821 acccgggcgt tcgccgaccg gtgcgagctg cagtgggcca acgacagttt cgacgatctg
     2881 ttgcagcacg tggacgcgat ctacatcgcc tcaccgcacg ccacccacca cgactacgcg
     2941 gtgcgggcga tcgacgccgg ggtgcacgtg ctgtgcgaga agccgatgac gctgtcgaag
     3001 aacgagaccc ggcagctgta cgagcgtgcc gcggcccgcc gggtggtgct ggtcgaagcg
     3061 atcaagacgg ccttctcgcc gggctttcaa cgcatggtcg cggtggcccg catcggcacc
     3121 atcggcaccg tccgctcggt cgatgccacg ttcaccaagc tggtcgacga gggccgcgag
     3181 ttgcacggca cggacggcgg cagcatctcg gagttggcca cctacccgct gctggcgatc
     3241 gtcaagctgc tcggcaccga tttcgacgag gtgcgcacgt actcgtaccg gcccgagggc
     3301 gccgaggtcg agttgttcag ccgtatcgac gtcacctacc cgcacgcgat cgcctcggcg
     3361 cgcaccggca tcggggtgaa ggccgagggt gatctggtgg tggccggcac caag
//
LOCUS       DAOSUG010000347         3409 bp    DNA     linear   ENV 10-JUN-2024
DEFINITION  MAG TPA_asm: Micropruina sp. isolate AMR_MDS_2195
            NODE_11348_length_3409_cov_3.473763, whole genome shotgun sequence.
ACCESSION   DAOSUG010000347 DAOSUG010000000
VERSION     DAOSUG010000347.1
DBLINK      BioProject: PRJNA925477
            BioSample: SAMN32864262
            Sequence Read Archive: SRR4236673
KEYWORDS    WGS; ENV; Metagenome Assembled Genome; MAG; Third Party Data; TPA;
            TPA:assembly.
SOURCE      Micropruina sp. (wastewater metagenome)
  ORGANISM  Micropruina sp.
            Bacteria; Bacillati; Actinomycetota; Actinomycetes;
            Propionibacteriales; Nocardioidaceae; Micropruina.
REFERENCE   1  (bases 1 to 3409)
  AUTHORS   Abdulkadir,N., Saraiva,J.P., Zhang,J., Stolte,S., Gillor,O.,
            Harms,H. and Rocha,U.
  TITLE     Genome-centric analyses of 165 metagenomes show that mobile genetic
            elements are crucial for the transmission of antimicrobial
            resistance genes to pathogens in activated sludge and wastewater
  JOURNAL   Microbiol Spectr 12 (3), e0291823 (2024)
   PUBMED   38289113
REFERENCE   2  (bases 1 to 3409)
  AUTHORS   Abdulkadir,N.
  TITLE     Direct Submission
  JOURNAL   Submitted (24-MAR-2023) Environmental Microbiology, Helmholtz
            Center for Environmental Research -UFZ, Permoserstr 15, Leipzig
            04318, Germany
COMMENT     The annotation was added by the NCBI Prokaryotic Genome Annotation
            Pipeline (PGAP). Information about PGAP can be found here:
            https://www.ncbi.nlm.nih.gov/genome/annotation_prok/
            
            ##Genome-Assembly-Data-START##
            Assembly Method        :: SPAdes v. 3.11.1
            Genome Representation  :: Full
            Expected Final Version :: Yes
            Genome Coverage        :: Not Applicable
            Sequencing Technology  :: Illumina MiSeq
            ##Genome-Assembly-Data-END##
            
            ##Genome-Annotation-Data-START##
            Annotation Provider               :: NCBI
            Annotation Date                   :: 05/04/2023 04:57:10
            Annotation Pipeline               :: NCBI Prokaryotic Genome
                                                 Annotation Pipeline (PGAP)
            Annotation Method                 :: Best-placed reference protein
                                                 set; GeneMarkS-2+
            Annotation Software revision      :: 6.5
            Features Annotated                :: Gene; CDS; rRNA; tRNA; ncRNA
            Genes (total)                     :: 2,776
            CDSs (total)                      :: 2,750
            Genes (coding)                    :: 2,714
            CDSs (with protein)               :: 2,714
            Genes (RNA)                       :: 26
            tRNAs                             :: 24
            ncRNAs                            :: 2
            Pseudo Genes (total)              :: 36
            CDSs (without protein)            :: 36
            Pseudo Genes (ambiguous residues) :: 0 of 36
            Pseudo Genes (frameshifted)       :: 2 of 36
            Pseudo Genes (incomplete)         :: 32 of 36
            Pseudo Genes (internal stop)      :: 2 of 36
            ##Genome-Annotation-Data-END##
FEATURES             Location/Qualifiers
     source          1..3409
                     /organism="Micropruina sp."
                     /mol_type="genomic DNA"
                     /submitter_seqid="NODE_11348_length_3409_cov_3.473763"
                     /isolate="AMR_MDS_2195"
                     /isolation_source="Activated sludge of municipal
                     wastewater treatment plants"
                     /db_xref="taxon:2737536"
                     /environmental_sample
                     /geo_loc_name="Canada"
                     /lat_lon="49.25 N 123.1 W"
                     /collection_date="2015-06-16"
                     /metagenome_source="wastewater metagenome"
                     /note="metagenomic"
     gene            <1..730
                     /locus_tag="PLF56_10785"
     CDS             <1..730
                     /locus_tag="PLF56_10785"
                     /inference="COORDINATES: protein motif:HMM:NF017044.2"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=2
                     /transl_table=11
                     /product="insulinase family protein"
                     /protein_id="HOQ54084.1"
                     /translation="RFFATHYAPNNAVLTLVGDVEPEDGFGRVTTMFGGLPRGVIGER
                     PDAPALPPMTGVPRREVVAAVPTDATYLTWRLPARDTRAYDAVELALAVLGDGQTSRL
                     HRRLVRTDGIAEGASASATGMIGGTSIGFASARALPGVSAEQLEITLLDELARFRDQG
                     PTAEEVDRAKAQYEREWLSELARFDSRADLFSAYATLHADPEAVNRRLDEVRSVSVEQ
                     VTEAARTYLEPDTRAVLTYRKVTS"
     gene            727..2055
                     /locus_tag="PLF56_10790"
     CDS             727..2055
                     /locus_tag="PLF56_10790"
                     /inference="COORDINATES: protein motif:HMM:NF017044.2"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_function: GO:0046872 - metal ion binding [Evidence
                     IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="pitrilysin family protein"
                     /protein_id="HOQ54085.1"
                     /translation="MKRPVIAAAQPWTFPTPRTVTLDNGLAVQAFHRPGQHLINATVV
                     LELPLQTEPAHIEGVTAILQRCLDEGTRSHPGTAYAEQLENRGAVLGGGLSHTATSVS
                     LEVPSTRFTEALPLFAEAITEPILDDADVRRHVALRQAEIEQQQAHPVHRGAQAFRAA
                     VIDAGCRASRPIAGSAGSVDGISGEAVRARYASEYGPSRGTLVLAGDFADDPLPLVAG
                     MFGEWHRPVATPVDEQPLPAQGPQVLLIDRPGAVQADVRLGTFGIDRTDPRWPALRLG
                     AFVLGGGFLSRLNRVLREQRGYTYGVQLANTPARHGGLLAMHASFRTDVAVPAIIEAR
                     ELMRVDGGRGITADELAEAVNFLIGITPLRCATAAGITDQVASLIEAGLDADFVNRHA
                     SQLQQVTAEQATEATATLLPPDGLAVVVVGDAEVLAGQLQSEGMTPQVIT"
     gene            complement(2166..>3409)
                     /locus_tag="PLF56_10795"
     CDS             complement(2166..>3409)
                     /locus_tag="PLF56_10795"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_013864024.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_component: GO:0016020 - membrane [Evidence IEA]"
                     /codon_start=3
                     /transl_table=11
                     /product="UPF0182 family protein"
                     /protein_id="HOQ54086.1"
                     /translation="LYNRTPKERVQEVAPWLTVDSNIYPALVDSRAVWIVDAYTTAST
                     FPNSELVNLRNSSADTQSRMVGAQVDENINYIRNSVKAVVDAYDGTVSLYAWDESDPI
                     LQSYERAFPGTVQPKSAISPDLHSHLRYPEDMFKVQREILGRYHMTNPYTWYAQSDLW
                     QVPDDPVKANSDAKEPPYYLSIKWPGDANPVFSQTTVFVPRLRSNLASYLSVVAEATS
                     PDYGKLRVLRMSDTHQIDGPGQTFNAINQDQKVAETLRPFLNQGAASATYGNLLTLPM
                     GNGLLYVAPVYTQRQGNNGSYPALAYVVVRFGSHVGIGSTLQQALDEVFSGDAGADTG
                     EKPVGTASASPSPSPSGSASPSPSSTATANPAAVALLDKAQTAFEAADAALRKGDLAT
                     YQSKVNEAKAALADALKLMGR"
BASE COUNT          518 a         1132 c         1217 g          542 t
ORIGIN      
        1 ccggttcttc gccacgcact acgcgcccaa caacgcggtc ctcaccctgg tcggcgacgt
       61 cgagccggag gacgggttcg gccgggtcac cacgatgttc ggaggtctgc cgcgaggggt
      121 gatcggcgaa cgtccggacg cacccgcatt gcccccgatg accggcgtgc cgcggcgcga
      181 ggtcgtcgcc gccgtcccaa ccgatgccac gtacctgacc tggcggttac cggcccgcga
      241 cacccgcgcc tacgacgccg tcgagttggc cctggcggtg ttgggcgatg gtcagacgtc
      301 tcggctgcac cgccggctcg tgcgcaccga cggcatcgct gagggggcga gcgcatccgc
      361 gaccggcatg atcgggggca ccagcatcgg cttcgcttcg gccagggcgt tgcccggggt
      421 gagcgccgag cagctggaga tcaccctgct ggacgaactg gcccggttcc gcgaccaggg
      481 cccgactgca gaggaggtcg accgggccaa ggcgcagtac gaacgcgagt ggctcagcga
      541 actcgccagg ttcgacagcc gagccgacct gttcagcgcc tacgccaccc ttcacgcaga
      601 ccccgaggcg gtcaaccggc ggttggacga ggtgcgatca gtgagcgtcg agcaggtcac
      661 cgaagccgca cgcacctacc tcgaacccga cacccgagcc gtgttgacct accgaaaggt
      721 gacctcatga agcgtccggt gatcgcagcc gcgcagccct ggacctttcc caccccgcgc
      781 accgtcacgc tcgacaacgg cctcgccgtg caggcgttcc atcggcccgg gcagcacctg
      841 atcaacgcca ccgtggtgct cgaactgccg ctgcagaccg agccggcgca catcgagggg
      901 gtgacggcca ttctgcagcg ctgcctggat gagggaaccc gcagccatcc cggcaccgcg
      961 tacgccgagc aactcgagaa ccggggtgcg gtgctgggcg gaggtctcag tcacacggcg
     1021 acgtcggtca gcctcgaggt gcccagcacc cgattcacgg aggcattgcc cctgttcgcc
     1081 gaggcgatca ccgagccgat cctggacgat gcggacgtgc gtcgccacgt cgcgcttcgg
     1141 caggccgaga tcgagcagca gcaggcgcat ccggtgcatc gcggagcgca agcgttccgg
     1201 gccgccgtga tcgacgcggg gtgccgcgcg tcccgtccga tcgcgggcag cgcgggcagc
     1261 gtggacggca tcagcggcga ggcggtgcgc gcccggtatg ccagcgaata cggaccctcg
     1321 cgaggcaccc tggtgctggc cggcgacttc gccgacgacc cgctgccgct ggtggcgggc
     1381 atgttcggtg agtggcatcg gccggtggcc acgccggtgg acgaacagcc cctgcccgcg
     1441 caaggcccgc aggtgctgct gatcgatcgt ccgggggcgg tgcaggcgga cgtgcggctg
     1501 ggcaccttcg gcatcgaccg taccgatcct cgatggccgg cactgcggct cggcgcgttc
     1561 gtactcggcg gcgggttctt gagccggctc aaccgggtgc tgcgcgaaca gcgtgggtac
     1621 acctacggcg tgcaactggc caacacgccg gcacgacacg gcggcctgct cgcgatgcac
     1681 gcgtcctttc gtaccgacgt ggcggtgccg gcgatcatcg aagcccgcga actgatgcgg
     1741 gtggacggcg ggcgcggcat cacggccgac gaactcgccg aggcggtcaa ctttctcatc
     1801 ggtatcacgc cgctgcgttg cgccactgcc gcgggcatca ccgatcaggt ggcgagcctg
     1861 atcgaggccg gactggacgc cgacttcgtc aatcggcatg cgtcgcagct tcagcaggtc
     1921 acggcagagc aggccaccga ggcgacggcg acgctgctgc ccccggacgg gctggctgtg
     1981 gtggtggtcg gtgacgcgga ggtgctcgcc ggtcagctgc agagcgaagg catgacgccg
     2041 caggtgatca cgtagcgtga cgggttgatc cgccgaacgc ccgcctaagt atcatgcgcc
     2101 cgcgtagctt cgcaggccgc ggatacttag gtgggcgttc gggctccagc gacgacgagc
     2161 cggcgtcagc gtcccatcag cttcagcgcg tcggcgagtg cggccttggc ctcgttcacc
     2221 ttggactggt aggtcgccag gtcgcccttg cggagcgccg cgtcggccgc ctcgaaggcc
     2281 gtttgcgcct tgtcgagcag cgccaccgcg gccgggttgg cggtcgccgt cgaactgggt
     2341 gatgggctgg ccgagccgga cgggctcggt gatgggctgg ccgaggccgt gccgaccggt
     2401 ttctcgccgg tgtcggcgcc cgcatcgccg gagaagacct cgtccagggc ctgctgcagc
     2461 gtgctgccga tgcccacgtg cgagccgaag cgcaccacca cgtacgccaa cgcggggtac
     2521 gaaccgttgt tgccctggcg ctgggtgtag accggagcca cgtacagcag cccgttaccc
     2581 atcggcaggg tcagcaggtt gccgtaggtg gccgatgcgg caccctggtt gaggaacgga
     2641 cgcagcgtct cggcaacctt ctgatcctgg ttgatggcgt tgaaggtctg cccggggccg
     2701 tcgatctggt gagtgtccga catgcgcagc acccgcagct tgccgtagtc gggactggtc
     2761 gcctcggcca ccacggacag atagctggcc aggttcgaac gcagccgcgg cacgaacacc
     2821 gtggtctggc tgaacaccgg attggcatcg ccgggccact tgatgctcag gtagtagggc
     2881 ggttctttcg cgtcggagtt ggccttgacc gggtcgtccg gcacctgcca caggtcggac
     2941 tgcgcgtacc aggtgtaggg gttggtcatg tggtagcggc cgagaatctc gcgctgcacc
     3001 ttgaacatgt cttcggggta gcgcaggtgg ctgtgcaggt cggggctgat cgcgctcttg
     3061 ggctgcacgg tgccggggaa cgcccgctcg tacgactgca gaatcgggtc ggactcgtcc
     3121 caggcgtaga gcgagaccgt gccgtcgtag gcgtcgacca cggccttgac cgagttgcga
     3181 atgtagttga tgttctcgtc gacctgcgcg cccaccatgc gcgactgggt gtcggctgag
     3241 gagttgcgca ggttcaccag ttcactgttc ggaaaggtgc tggcggtggt gtaggcgtcc
     3301 acgatccaga ccgcccggct gtcgaccagc gccgggtaga tgttcgagtc gacggtcagc
     3361 cagggcgcca cctcttgcac ccgctctttg ggggtgcggt tgtacagaa
//
LOCUS       DAOSUG010000348         3407 bp    DNA     linear   ENV 10-JUN-2024
DEFINITION  MAG TPA_asm: Micropruina sp. isolate AMR_MDS_2195
            NODE_11357_length_3407_cov_4.607100, whole genome shotgun sequence.
ACCESSION   DAOSUG010000348 DAOSUG010000000
VERSION     DAOSUG010000348.1
DBLINK      BioProject: PRJNA925477
            BioSample: SAMN32864262
            Sequence Read Archive: SRR4236673
KEYWORDS    WGS; ENV; Metagenome Assembled Genome; MAG; Third Party Data; TPA;
            TPA:assembly.
SOURCE      Micropruina sp. (wastewater metagenome)
  ORGANISM  Micropruina sp.
            Bacteria; Bacillati; Actinomycetota; Actinomycetes;
            Propionibacteriales; Nocardioidaceae; Micropruina.
REFERENCE   1  (bases 1 to 3407)
  AUTHORS   Abdulkadir,N., Saraiva,J.P., Zhang,J., Stolte,S., Gillor,O.,
            Harms,H. and Rocha,U.
  TITLE     Genome-centric analyses of 165 metagenomes show that mobile genetic
            elements are crucial for the transmission of antimicrobial
            resistance genes to pathogens in activated sludge and wastewater
  JOURNAL   Microbiol Spectr 12 (3), e0291823 (2024)
   PUBMED   38289113
REFERENCE   2  (bases 1 to 3407)
  AUTHORS   Abdulkadir,N.
  TITLE     Direct Submission
  JOURNAL   Submitted (24-MAR-2023) Environmental Microbiology, Helmholtz
            Center for Environmental Research -UFZ, Permoserstr 15, Leipzig
            04318, Germany
COMMENT     The annotation was added by the NCBI Prokaryotic Genome Annotation
            Pipeline (PGAP). Information about PGAP can be found here:
            https://www.ncbi.nlm.nih.gov/genome/annotation_prok/
            
            ##Genome-Assembly-Data-START##
            Assembly Method        :: SPAdes v. 3.11.1
            Genome Representation  :: Full
            Expected Final Version :: Yes
            Genome Coverage        :: Not Applicable
            Sequencing Technology  :: Illumina MiSeq
            ##Genome-Assembly-Data-END##
            
            ##Genome-Annotation-Data-START##
            Annotation Provider               :: NCBI
            Annotation Date                   :: 05/04/2023 04:57:10
            Annotation Pipeline               :: NCBI Prokaryotic Genome
                                                 Annotation Pipeline (PGAP)
            Annotation Method                 :: Best-placed reference protein
                                                 set; GeneMarkS-2+
            Annotation Software revision      :: 6.5
            Features Annotated                :: Gene; CDS; rRNA; tRNA; ncRNA
            Genes (total)                     :: 2,776
            CDSs (total)                      :: 2,750
            Genes (coding)                    :: 2,714
            CDSs (with protein)               :: 2,714
            Genes (RNA)                       :: 26
            tRNAs                             :: 24
            ncRNAs                            :: 2
            Pseudo Genes (total)              :: 36
            CDSs (without protein)            :: 36
            Pseudo Genes (ambiguous residues) :: 0 of 36
            Pseudo Genes (frameshifted)       :: 2 of 36
            Pseudo Genes (incomplete)         :: 32 of 36
            Pseudo Genes (internal stop)      :: 2 of 36
            ##Genome-Annotation-Data-END##
FEATURES             Location/Qualifiers
     source          1..3407
                     /organism="Micropruina sp."
                     /mol_type="genomic DNA"
                     /submitter_seqid="NODE_11357_length_3407_cov_4.607100"
                     /isolate="AMR_MDS_2195"
                     /isolation_source="Activated sludge of municipal
                     wastewater treatment plants"
                     /db_xref="taxon:2737536"
                     /environmental_sample
                     /geo_loc_name="Canada"
                     /lat_lon="49.25 N 123.1 W"
                     /collection_date="2015-06-16"
                     /metagenome_source="wastewater metagenome"
                     /note="metagenomic"
     gene            complement(16..762)
                     /locus_tag="PLF56_10800"
     CDS             complement(16..762)
                     /locus_tag="PLF56_10800"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_013861603.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="fructosamine kinase family protein"
                     /protein_id="HOQ54087.1"
                     /translation="MADPTYTKTRADAPDGFFAAEAAGLRWLAVKDGAAVAGVHAVSR
                     TTLVIDRVATASASAAAAEAFGRALARTHDAGAAFFGVGPAGGPGRGWIGDAPLPLRT
                     EPPTGWGAWYAEDRVLFHARRAGIDDPALRRLVARLSAGDFDDDAPPARVHGDLWSGN
                     VLWSPAGVVLIDPAAHGGHRLTDLAMLALFGTPHLNRIHAAYEQECVHLPDGWRDLIG
                     LHQLHPLLVHAELFGGGYARQALDVAERFA"
     gene            complement(1084..1479)
                     /locus_tag="PLF56_10805"
     CDS             complement(1084..1479)
                     /locus_tag="PLF56_10805"
                     /inference="COORDINATES: ab initio
                     prediction:GeneMarkS-2+"
                     /note="Derived by automated computational analysis using
                     gene prediction method: GeneMarkS-2+."
                     /codon_start=1
                     /transl_table=11
                     /product="hypothetical protein"
                     /protein_id="HOQ54088.1"
                     /translation="MPAYAGTRWDARFDQSLLGAEGAPAGDAGELGVFALADDAGVLG
                     VFAVTGDAGVLGVFAVTGDAGVFAVFAVAGDAGVFAVFAVAGDAGVFAVVALGAVVAM
                     GALLLPGSACCPATAEATSPAGTEVGAWP"
     gene            1585..2757
                     /locus_tag="PLF56_10810"
     CDS             1585..2757
                     /locus_tag="PLF56_10810"
                     /inference="COORDINATES: ab initio
                     prediction:GeneMarkS-2+"
                     /note="Derived by automated computational analysis using
                     gene prediction method: GeneMarkS-2+."
                     /codon_start=1
                     /transl_table=11
                     /product="hypothetical protein"
                     /protein_id="HOQ54089.1"
                     /translation="MSIEKALALITSPEVGEVLQLALTGDPNVRLAAFGFELDRVHHR
                     PGADVSAVFRIWYDLPESDHPTEEWLGLTTAPAQQGVATLIHNDLVLRAWRHPADPML
                     TALPAACDAEVVAGWLGAPVQLEMLAYRPLRRAVLAARGARPAFLKVLRPHQADALAQ
                     RQGMLHAAGLTPAVLGRPEPGVVISAVAQGSSLAHALVAWPQDPGRLPSPGSLVALLD
                     RLPAEVLGLPRRDSWSDRIDFHAAGAAVVLPDQADEIRGLAADLAQLLAGVPTGPVVP
                     THGDFYEANIFTADGAATTVIDVDSVGPGRRDDDLACLLGHVSVLPNLSPEHYPRVAE
                     VLETWRAEFERHVHPASLRARTAAVILSLVSGTDTPTALARLDLARAWLVRARQAA"
     gene            complement(2766..3227)
                     /locus_tag="PLF56_10815"
     CDS             complement(2766..3227)
                     /locus_tag="PLF56_10815"
                     /inference="COORDINATES: ab initio
                     prediction:GeneMarkS-2+"
                     /note="Derived by automated computational analysis using
                     gene prediction method: GeneMarkS-2+."
                     /codon_start=1
                     /transl_table=11
                     /product="hypothetical protein"
                     /protein_id="HOQ54090.1"
                     /translation="MAAQPVPEPTTHGVAAEGGLTADWGEAGDTWGAGRSPRRCAEAL
                     VGAVALVDPLALLDAVALVDPLALLDPLALLDAVALVGETDAGAASARGDALITTAGP
                     TCSGGSPSPWAHPPTPSAARPSTPLTSHRPVIVVRMDASGSPRDERAASAG"
BASE COUNT          497 a         1217 c         1207 g          486 t
ORIGIN      
        1 ccgtccgctc gccgctcagg cgaagcgctc ggcgacgtcg agggcctgac gcgcgtaccc
       61 cccgccgaac aactcggcgt gcaccaacag gggatgcagt tgatgcaggc cgatcaggtc
      121 gcgccagccg tccggcaggt gcacgcactc ctgctcgtag gcggcgtgaa tgcggttcag
      181 gtgcggggtg ccgaacaggg ccagcatggc cagatcggtg aggcggtggc cgccgtgcgc
      241 cgcggggtcg atcagcacca cgccggcggg cgaccagagc acattgcccg accacaggtc
      301 accatgcacc cgggccggag gagcgtcgtc gtcgaagtcg ccggcgctca accgggcgac
      361 cagccggcgt agcgccgggt cgtcgatgcc ggcccggcgc gcgtggaaca gcacgcgatc
      421 ctcggcgtac cacgcgcccc agccggtcgg gggctcggtg cgcagcggca gcggagcatc
      481 gccgatccaa ccgcggccgg ggcctcctgc cggaccgaca ccgaagaacg ccgcgccggc
      541 atcgtgcgtg cgcgccaagg cgcgtccaaa ggcttcggcg gccgctgctg aggccgatgc
      601 ggtggcgacg cggtcgatga ccagggtcgt ccgcgagaca gcgtgcacac cggcgacggc
      661 cgcgccgtcc ttcaccgcga gccagcgcag tcccgcagcc tcagccgcga agaagccgtc
      721 gggggcgtcc gcgcgggtct tggtgtaggt cggatcggcc atgccgcgag cgtagtgaca
      781 tctgtgcggc ccgcgcaggc tgggtgtggt tggacgggcg tcacgatgct cggcgccgag
      841 ttgggcaccg gagctgaccc gcggcacaag gacggatccg ggtcgcatcg tacggccacg
      901 ttccgtgaga gcactctcac gtcgttctcc gcgaaggttc accgccggtt gatgaagtgg
      961 ttctcacgca accaaccgaa aggaacccat catgaggatg ctccagaagt ccaccgccac
     1021 ctggatggtg acgggggcgc tcggcctcag cgccttcggc ggtgccgctt acgcgttcgc
     1081 cgatcaaggc caggcaccca cctcggtgcc cgcaggactt gtcgcctccg ccgtcgccgg
     1141 gcaacaggcc gagccgggca gcaatagcgc tcccatggcc accaccgccc cgagcgccac
     1201 taccgcgaac acgccggcgt cgcccgccac ggcgaacacg gcgaacacgc cggcgtcgcc
     1261 cgccacggcg aacacggcga acacgccggc gtcacccgtg acggcgaaca cgcccaacac
     1321 gccggcgtca cccgtgacgg cgaacacgcc caacacgccg gcatcgtctg cgagcgcgaa
     1381 cacccccaac tcaccggcat cgccggcggg cgccccctcg gcgccgagca acgactgatc
     1441 gaaccgagcg tcccaccggg tgcccgcgta agcgggcatc cggcatgcta ggggcgtgtc
     1501 gcacacctca cgccatgcat cgatgcgtag gcctgcccct ggtggtcggg tccacggtcg
     1561 tccgacccgt ggcagggggc atcggtgagc attgagaagg cgttggcact gatcacctcg
     1621 cctgaggtcg gcgaggtgct gcagctggca ctgacgggcg acccgaacgt gcggttggcc
     1681 gccttcggct tcgaactcga tcgggtgcac caccgtccgg gagccgatgt cagtgcggtc
     1741 ttccggatct ggtacgacct cccggaatcc gatcacccca ccgaggaatg gctcggtctg
     1801 accaccgcgc cggcgcagca gggggtggcg accctgattc acaacgacct ggtgttgcgg
     1861 gcctggcggc atccggccga cccgatgctg accgcactgc ccgcggcctg cgacgccgag
     1921 gtggtggcgg gatggctggg cgcaccggtg cagctggaga tgctcgccta ccgtccgctg
     1981 cgacgggcgg tgctggccgc acggggggcg cgtccggcgt ttctgaaggt gttgcgtccc
     2041 catcaggcgg acgctctcgc gcagcgacag ggcatgcttc acgccgccgg tctcacccct
     2101 gccgtgctcg gccggcccga gccgggggtg gtgatcagcg cggtggcgca gggcagttcg
     2161 ctcgcccacg ccctggtggc gtggccgcag gatccgggcc ggctgccctc gcccggttcg
     2221 ctggtcgcct tgctggaccg cctgcctgcc gaggtgctgg ggttgcccag gcgtgactcg
     2281 tggagcgacc ggatcgactt tcacgcggcc ggcgccgcgg tggtgctgcc cgatcaggcg
     2341 gacgagatcc gagggctggc ggccgatttg gcgcagttgc tggccggcgt tcccaccggg
     2401 cccgtggtgc cgacccacgg ggacttctac gaggccaaca tcttcaccgc cgacggggcg
     2461 gcgacaacgg tgatcgacgt cgattcggtc gggcccggac gccgcgacga cgacctcgcg
     2521 tgcctgctgg gtcacgtgtc ggtgctgccc aacttgtcgc ccgagcacta tccacgggtg
     2581 gccgaggtgc ttgagacctg gcgcgcggag ttcgagcggc acgtacatcc cgcctctttg
     2641 cgggccagga ctgcggcggt catcctgagt ctggtctctg gcaccgacac cccgaccgcg
     2701 ttggccaggc tcgacctcgc ccgggcctgg ctggtgcggg cacgtcaggc cgcatgagag
     2761 gtttctcatc cagcgctcgc agctcgctca tcacgcggtg atccactggc gtccatgcgc
     2821 acaacgatca ccggtcggtg gctggtcagt ggcgtgctcg gcctcgccgc actcggcgtt
     2881 ggcggatgtg cccagggcga gggtgagcca cccgagcagg tcggcccggc ggtggtgatc
     2941 agcgcgtccc cgcgtgccga agccgcgcct gcatcggtct cgccgaccag cgcgaccgcg
     3001 tcgagcagtg cgagcgggtc gagcagtgcg agcgggtcga ctagcgcgac cgcgtcgagc
     3061 agtgcgagcg ggtcgaccag cgcgaccgcg ccgaccaagg cctctgcgca acgcctgggt
     3121 gatcggcccg ctccccaggt gtcgccggcc tcgccccaat cggccgtcag cccaccctcg
     3181 gcggcgacgc catgagttgt cggttcgggc accggctgag cggccatccc gcatccggtc
     3241 catcgcgggc gttcggcagt tgcgcggttt gttgttgagc gtgtcgaaca cgctggtcgg
     3301 cacactcgac cagcaacaaa tcggcgtgct gccaaccaac tgcgatcaac gcgccaccca
     3361 tcggttgagc ttcgagaacc cctggtcgca ggggcctcgg cacgctc
//
LOCUS       DAOSUG010000349         3383 bp    DNA     linear   ENV 10-JUN-2024
DEFINITION  MAG TPA_asm: Micropruina sp. isolate AMR_MDS_2195
            NODE_11470_length_3383_cov_4.177284, whole genome shotgun sequence.
ACCESSION   DAOSUG010000349 DAOSUG010000000
VERSION     DAOSUG010000349.1
DBLINK      BioProject: PRJNA925477
            BioSample: SAMN32864262
            Sequence Read Archive: SRR4236673
KEYWORDS    WGS; ENV; Metagenome Assembled Genome; MAG; Third Party Data; TPA;
            TPA:assembly.
SOURCE      Micropruina sp. (wastewater metagenome)
  ORGANISM  Micropruina sp.
            Bacteria; Bacillati; Actinomycetota; Actinomycetes;
            Propionibacteriales; Nocardioidaceae; Micropruina.
REFERENCE   1  (bases 1 to 3383)
  AUTHORS   Abdulkadir,N., Saraiva,J.P., Zhang,J., Stolte,S., Gillor,O.,
            Harms,H. and Rocha,U.
  TITLE     Genome-centric analyses of 165 metagenomes show that mobile genetic
            elements are crucial for the transmission of antimicrobial
            resistance genes to pathogens in activated sludge and wastewater
  JOURNAL   Microbiol Spectr 12 (3), e0291823 (2024)
   PUBMED   38289113
REFERENCE   2  (bases 1 to 3383)
  AUTHORS   Abdulkadir,N.
  TITLE     Direct Submission
  JOURNAL   Submitted (24-MAR-2023) Environmental Microbiology, Helmholtz
            Center for Environmental Research -UFZ, Permoserstr 15, Leipzig
            04318, Germany
COMMENT     The annotation was added by the NCBI Prokaryotic Genome Annotation
            Pipeline (PGAP). Information about PGAP can be found here:
            https://www.ncbi.nlm.nih.gov/genome/annotation_prok/
            
            ##Genome-Assembly-Data-START##
            Assembly Method        :: SPAdes v. 3.11.1
            Genome Representation  :: Full
            Expected Final Version :: Yes
            Genome Coverage        :: Not Applicable
            Sequencing Technology  :: Illumina MiSeq
            ##Genome-Assembly-Data-END##
            
            ##Genome-Annotation-Data-START##
            Annotation Provider               :: NCBI
            Annotation Date                   :: 05/04/2023 04:57:10
            Annotation Pipeline               :: NCBI Prokaryotic Genome
                                                 Annotation Pipeline (PGAP)
            Annotation Method                 :: Best-placed reference protein
                                                 set; GeneMarkS-2+
            Annotation Software revision      :: 6.5
            Features Annotated                :: Gene; CDS; rRNA; tRNA; ncRNA
            Genes (total)                     :: 2,776
            CDSs (total)                      :: 2,750
            Genes (coding)                    :: 2,714
            CDSs (with protein)               :: 2,714
            Genes (RNA)                       :: 26
            tRNAs                             :: 24
            ncRNAs                            :: 2
            Pseudo Genes (total)              :: 36
            CDSs (without protein)            :: 36
            Pseudo Genes (ambiguous residues) :: 0 of 36
            Pseudo Genes (frameshifted)       :: 2 of 36
            Pseudo Genes (incomplete)         :: 32 of 36
            Pseudo Genes (internal stop)      :: 2 of 36
            ##Genome-Annotation-Data-END##
FEATURES             Location/Qualifiers
     source          1..3383
                     /organism="Micropruina sp."
                     /mol_type="genomic DNA"
                     /submitter_seqid="NODE_11470_length_3383_cov_4.177284"
                     /isolate="AMR_MDS_2195"
                     /isolation_source="Activated sludge of municipal
                     wastewater treatment plants"
                     /db_xref="taxon:2737536"
                     /environmental_sample
                     /geo_loc_name="Canada"
                     /lat_lon="49.25 N 123.1 W"
                     /collection_date="2015-06-16"
                     /metagenome_source="wastewater metagenome"
                     /note="metagenomic"
     gene            complement(<1..147)
                     /locus_tag="PLF56_10820"
     CDS             complement(<1..147)
                     /locus_tag="PLF56_10820"
                     /inference="COORDINATES: protein motif:HMM:NF015862.2"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="heme-binding protein"
                     /protein_id="HOQ54091.1"
                     /translation="MSGEKIADSLSLEDARRVIDAAVAKAVEIGVPQDVAVVDTGGHL
                     KAHVR"
     gene            complement(254..1249)
                     /gene="dhaK"
                     /locus_tag="PLF56_10825"
     CDS             complement(254..1249)
                     /gene="dhaK"
                     /locus_tag="PLF56_10825"
                     /EC_number="2.7.1.121"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_016455048.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_function: GO:0004371 - glycerone kinase activity
                     [Evidence IEA];
                     GO_process: GO:0006071 - glycerol metabolic process
                     [Evidence IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="dihydroxyacetone kinase subunit DhaK"
                     /protein_id="HOQ54092.1"
                     /translation="MKKFVNDPKQFVPEMLKGIALANPDTITYVPEYNLIMRSDAPNN
                     DKVSIVQGSGSGHEPAHVMAVGPGMLDAACPGDVFAAPPADYVYQTAKLVASDKGVLL
                     LVNNYTGDRMAFEMAQELAEADDIKCATLFIDDDVAVQDSLYTVGRRGVAGNFFVIKA
                     VGAKAEEGADLDEVIRVGEKVNSVTRTMGIALSSCTPPAKGQPLFEIGDDEIEVGIGI
                     HGEPGRRRAKMMSANEIVDELLGAVVPDLPFESGDRVALMINGLGGTPISELYLLFGI
                     AAQKLADQGITVARSYVGEYCTSLEMAGCSLTLVKLDDEIEALLEAPAEIVARVF"
     gene            complement(1348..1941)
                     /gene="dhaL"
                     /locus_tag="PLF56_10830"
                     /pseudo
     CDS             complement(1348..1941)
                     /gene="dhaL"
                     /locus_tag="PLF56_10830"
                     /inference="COORDINATES: protein motif:HMM:TIGR02365.1"
                     /note="incomplete; partial in the middle of a contig;
                     missing N-terminus; Derived by automated computational
                     analysis using gene prediction method: Protein Homology.
                     GO_function: GO:0004371 - glycerone kinase activity
                     [Evidence IEA];
                     GO_process: GO:0006071 - glycerol metabolic process
                     [Evidence IEA]"
                     /pseudo
                     /codon_start=1
                     /transl_table=11
                     /product="dihydroxyacetone kinase subunit DhaL"
     gene            complement(2156..2656)
                     /locus_tag="PLF56_10835"
     CDS             complement(2156..2656)
                     /locus_tag="PLF56_10835"
                     /inference="COORDINATES: protein motif:HMM:NF012422.2"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_function: GO:0003677 - DNA binding [Evidence IEA];
                     GO_process: GO:0000160 - phosphorelay signal transduction
                     system [Evidence IEA];
                     GO_process: GO:0006355 - regulation of DNA-templated
                     transcription [Evidence IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="response regulator transcription factor"
                     /protein_id="HOQ54093.1"
                     /translation="MDELEAAVEAVQLLRPDVVLASTALACPSGKPVVTHLRGALPDV
                     AVIGVLHGSEGELLAWAAAGARGFVPADVDATGLGRAVVAAARGEALLAGDLLEQLGG
                     LPKLDEASRLTQREAEVRDLINEGLADKQIALRLGISAKTVEKHVGAVLRKAGVRSRT
                     ELLARV"
     gene            complement(2713..3108)
                     /locus_tag="PLF56_10840"
     CDS             complement(2713..3108)
                     /locus_tag="PLF56_10840"
                     /inference="COORDINATES: protein motif:HMM:NF024793.2"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="hypothetical protein"
                     /protein_id="HOQ54094.1"
                     /translation="MAPTALELLRDMHDRTRLALIFIGMPGIDQRFRHYPQLFSRLGF
                     SHRYRPLGRDELLFVLERHWKRLGRTLNPDDFTDAQAIAAVERVTRGNFRLLERLFPQ
                     ISRVLKINQLETITDDVIEAAASTLVVGA"
     gene            3070..>3383
                     /locus_tag="PLF56_10845"
     CDS             3070..>3383
                     /locus_tag="PLF56_10845"
                     /inference="COORDINATES: ab initio
                     prediction:GeneMarkS-2+"
                     /note="Derived by automated computational analysis using
                     gene prediction method: GeneMarkS-2+."
                     /codon_start=1
                     /transl_table=11
                     /product="hypothetical protein"
                     /protein_id="HOQ54095.1"
                     /translation="MHVAEQFQGGGGQPFGFINDQQLDPAGCVAGAGIGDFTDGSLMI
                     VNADAEADDPMVDVADQLAISGQLCKLPRTCTSPLQTSSDIHKVSSILRSTAALVVVV
                     AES"
BASE COUNT          504 a         1087 c         1168 g          624 t
ORIGIN      
        1 gcgcacgtgg gccttcagat ggccgccggt gtcgaccacc gccacgtcct gcggcacacc
       61 gatctcgacg gccttggcca ccgcggcatc gatcacgcgg cgggcgtcct ccagcgatag
      121 gctgtcggcg atcttctctc cactcatgtc ctgctccttc gcatgtgggt ttctgggtgg
      181 ggttcggggg tcaggtaccc ggccctccga ctggcctcgg aggtgtccgg gtaccgcttg
      241 tttcggccga ggctcagaag acccgggcca cgatctcggc cggtgcctcc agcagcgcct
      301 cgatctcgtc gtcgagcttc accagggtga gtgagcagcc ggccatctcg aggctggtgc
      361 agtactcacc gacgtagctc ctcgccacgg tgatgccctg gtcggccagc ttctgcgcgg
      421 cgatgccgaa cagcaggtac agctcgctga tcggggtacc gcccagaccg ttgatcatca
      481 acgcgacccg gtcgcccgac tcgaacggca ggtcgggcac caccgcgccg agcagctcgt
      541 cgacgatctc gttggcgctc atcatcttgg cccggcgccg acccggctcg ccgtggatgc
      601 cgatgccgac ctcgatctcg tcgtcgccga tctcgaacag cggctggccc ttggccggcg
      661 gggtgcacga gctgagcgcg atgcccatgg tgcgggtgac actgttcacc ttctcgccga
      721 cccggatcac ttcgtcgagg tccgcgccct cttcggcctt ggcaccgacg gccttgatca
      781 cgaagaagtt gccggccacg ccccggcgtc cgacggtgta gagcgagtcc tgcacggcca
      841 cgtcgtcgtc gatgaacagg gtggcgcact tgatgtcgtc ggcctcggcc agctcttggg
      901 ccatctcgaa ggccatccgg tcaccggtgt agttgttcac cagcagcagc acgcccttgt
      961 cggacgccac cagcttggcg gtctggtaca cgtagtcggc cggcggggcg gcgaacacgt
     1021 caccggggca ggcggcgtcc agcatgccgg ggccgaccgc catcacatgg gccggttcgt
     1081 ggcccgagcc cgagccctgc acgatgctga ccttgtcgtt gttcggggcg tccgaacgca
     1141 tgatcaggtt gtactccggc acgtaggtga tcgtgtcggg gttggccagc gcgatgccct
     1201 tgagcatctc gggcacgaac tgcttggggt cgttgacaaa cttcttcatt gcgttccctt
     1261 cgttgggtga cggtgcggtg gagggtgcgg acgcgacgct gtcccgtccg ggtctatgga
     1321 aggcccgcgg tcgcgggcag gggcggctca gcccttgggc cactgctccg cgagcgcttc
     1381 gagcagcacg gcgatggctg tcgcaccggc gtcgaccgaa ccgatgctgc gctcgccggt
     1441 gtaactggcc cggccgcggc gggcctgcag cttggcggtg tcatcggcgg cctgacgggc
     1501 ggtgacggcc attgctgcga cggcgtcggc ggcgctcgct ccggcgttga tctgctcttc
     1561 gagggtgtcg atggcgggca ccagcgagtc gagcagcgtc ttgtcgccga gttcggcctt
     1621 gccgcgggcc ttgatgcctt cgacggccgc gcgcagtccc ttgatcacgt cggcggcctc
     1681 gaacgtcggc ttgcccttca ggttggtacc cacgcgcagg atcgcggtgc tccagatcgg
     1741 gcccgaggta ccgccgatgc gagccgagat cgtcatcgcc accttggtca ggaacacgcc
     1801 gccatcggtg cggtcgtagc tgtcccagtc ctgtttgacg atctcgaagc cgcgggcgag
     1861 ggagtagccg aagtcaccgt cgcccgcgac ggcgtccagc tcgccgaagt agttttcgtt
     1921 ttcgagcacc gtgtcgggtg accgtgttga ccacgaactc gacgtcggaa agcgaaccgg
     1981 tcatgcattt ctcctttgaa agctgcctgc ggcccaggtg cgccaccggg gatccggccg
     2041 ctcgactgcg tcgtcgaacc gtgccgcgct gattgatgag accaacctag gtgcgtggcg
     2101 gacgtagggg aatggggact ttccccaccg gccggggtag ggatcgcatg gggaatcaga
     2161 ctcgggccaa cagttcggtg cggctgcgca ccccagcctt gcgcagtacc gcgcccacgt
     2221 gtttctcgac cgtcttcgcc gaaataccta gccgaagtgc gatctgcttg tcggcaagac
     2281 cctcgttgat caggtcgcga acctcggcct cgcgctgggt cagccgcgag gcctcatcga
     2341 gtttgggcaa cccgccgagc tgttcgagca ggtcaccggc gagcagcgcc tcaccgcggg
     2401 cggccgccac cacggctcgt cccagaccgg tggcgtcgac gtcggccggc acaaagcccc
     2461 gtgcaccggc ggccgcccac gccagcaact cgccctcgga accgtgcaac accccgatca
     2521 ccgccacgtc aggcagggca ccccgcagat gcgtgaccac cggcttgccg gacgggcagg
     2581 ccagggccgt gctggccagc accacgtccg gccgcagcag ctggacggcc tcgaccgccg
     2641 cctcgagctc gtccacctcg gctgtggttc acatcgctat gtggcggtgt tctgttgatc
     2701 gttgggtgac ggtcaggcgc ctacgacgag ggtgctggcg gctgcttcga tgacgtcgtc
     2761 ggtgatcgtt tcgagttggt tgatcttgag aacacggctg atctggggga agagtcgttc
     2821 gagaagtcgg aaattgccgc gggtgacgcg ttcgacggcg gcgatggctt gggcgtcggt
     2881 gaagtcgtcg gggttgaggg ttcggcctag tcgtttccag tggcgctcga ggacgaagag
     2941 gagttcgtcg cgaccgaggg ggcggtatcg gtgagagaag ccgaggcggc tgaacagctg
     3001 ggggtagtgg cggaagcgtt ggtcgatgcc gggcatgccg atgaagatca gggcgagtcg
     3061 ggtgcggtcg tgcatgtcgc ggagcagttc cagggcggtg ggggccagcc gttcggcttc
     3121 atcaatgatc agcagctcga cccagcgggt tgcgtcgccg gcgcgggtat cggcgatttt
     3181 accgatggct cgttgatgat cgtcaatgca gacgccgagg cggacgaccc aatggtcgat
     3241 gtcgcggatc agctcgcgat ttcggggcag ctctgcaaac tgccacgaac gtgcacgtca
     3301 cctttgcaga cgagttcgga cattcacaaa gtgtcaagca tcctgaggag tacggcagcc
     3361 ctcgtcgtcg ttgtagcgga atc
//
LOCUS       DAOSUG010000350         3376 bp    DNA     linear   ENV 10-JUN-2024
DEFINITION  MAG TPA_asm: Micropruina sp. isolate AMR_MDS_2195
            NODE_11506_length_3376_cov_3.791328, whole genome shotgun sequence.
ACCESSION   DAOSUG010000350 DAOSUG010000000
VERSION     DAOSUG010000350.1
DBLINK      BioProject: PRJNA925477
            BioSample: SAMN32864262
            Sequence Read Archive: SRR4236673
KEYWORDS    WGS; ENV; Metagenome Assembled Genome; MAG; Third Party Data; TPA;
            TPA:assembly.
SOURCE      Micropruina sp. (wastewater metagenome)
  ORGANISM  Micropruina sp.
            Bacteria; Bacillati; Actinomycetota; Actinomycetes;
            Propionibacteriales; Nocardioidaceae; Micropruina.
REFERENCE   1  (bases 1 to 3376)
  AUTHORS   Abdulkadir,N., Saraiva,J.P., Zhang,J., Stolte,S., Gillor,O.,
            Harms,H. and Rocha,U.
  TITLE     Genome-centric analyses of 165 metagenomes show that mobile genetic
            elements are crucial for the transmission of antimicrobial
            resistance genes to pathogens in activated sludge and wastewater
  JOURNAL   Microbiol Spectr 12 (3), e0291823 (2024)
   PUBMED   38289113
REFERENCE   2  (bases 1 to 3376)
  AUTHORS   Abdulkadir,N.
  TITLE     Direct Submission
  JOURNAL   Submitted (24-MAR-2023) Environmental Microbiology, Helmholtz
            Center for Environmental Research -UFZ, Permoserstr 15, Leipzig
            04318, Germany
COMMENT     The annotation was added by the NCBI Prokaryotic Genome Annotation
            Pipeline (PGAP). Information about PGAP can be found here:
            https://www.ncbi.nlm.nih.gov/genome/annotation_prok/
            
            ##Genome-Assembly-Data-START##
            Assembly Method        :: SPAdes v. 3.11.1
            Genome Representation  :: Full
            Expected Final Version :: Yes
            Genome Coverage        :: Not Applicable
            Sequencing Technology  :: Illumina MiSeq
            ##Genome-Assembly-Data-END##
            
            ##Genome-Annotation-Data-START##
            Annotation Provider               :: NCBI
            Annotation Date                   :: 05/04/2023 04:57:10
            Annotation Pipeline               :: NCBI Prokaryotic Genome
                                                 Annotation Pipeline (PGAP)
            Annotation Method                 :: Best-placed reference protein
                                                 set; GeneMarkS-2+
            Annotation Software revision      :: 6.5
            Features Annotated                :: Gene; CDS; rRNA; tRNA; ncRNA
            Genes (total)                     :: 2,776
            CDSs (total)                      :: 2,750
            Genes (coding)                    :: 2,714
            CDSs (with protein)               :: 2,714
            Genes (RNA)                       :: 26
            tRNAs                             :: 24
            ncRNAs                            :: 2
            Pseudo Genes (total)              :: 36
            CDSs (without protein)            :: 36
            Pseudo Genes (ambiguous residues) :: 0 of 36
            Pseudo Genes (frameshifted)       :: 2 of 36
            Pseudo Genes (incomplete)         :: 32 of 36
            Pseudo Genes (internal stop)      :: 2 of 36
            ##Genome-Annotation-Data-END##
FEATURES             Location/Qualifiers
     source          1..3376
                     /organism="Micropruina sp."
                     /mol_type="genomic DNA"
                     /submitter_seqid="NODE_11506_length_3376_cov_3.791328"
                     /isolate="AMR_MDS_2195"
                     /isolation_source="Activated sludge of municipal
                     wastewater treatment plants"
                     /db_xref="taxon:2737536"
                     /environmental_sample
                     /geo_loc_name="Canada"
                     /lat_lon="49.25 N 123.1 W"
                     /collection_date="2015-06-16"
                     /metagenome_source="wastewater metagenome"
                     /note="metagenomic"
     gene            complement(155..1495)
                     /locus_tag="PLF56_10850"
     CDS             complement(155..1495)
                     /locus_tag="PLF56_10850"
                     /inference="COORDINATES: ab initio
                     prediction:GeneMarkS-2+"
                     /note="Derived by automated computational analysis using
                     gene prediction method: GeneMarkS-2+."
                     /codon_start=1
                     /transl_table=11
                     /product="hypothetical protein"
                     /protein_id="HOQ54096.1"
                     /translation="MVRLVIPVLVALAMFAAVMLTIDAGRRQRARAIARDQRTRRAAQ
                     VSLTAAPAPDPDDAWLPVERSLAAPPVRLPSGGVRVELRPDPGNPDAVAVFVGDQLVG
                     RLPERVTGAYRGRLSTNNGALGAVGLNADGLLTLVPVGLTRPTPPQYLHLVQAWGWST
                     TAVSSDRVFVYLPGVAQVFARAERPITEAATTLDDLTAALLLDDEGFVDVLVGDAWIG
                     SLAEPDAEQIRPALQRLARTGGLLQVGMRLVARRRSGRLLCRVTLRLPDPNQINPPGP
                     LPDAPFVLLPPVTTLQVTGEKAALDLLAPLLGSGAQASVVVTLHTADGLDDASAPHLT
                     QLDGPGDEPLGEAVGATDVAVPVDADRVRAGARLTPAEPSRVEVRVDGRPVGLLSAAG
                     SAATHGLVRACEEAGRVPVARGLVNGNSLAVTMAVRTARTWDVTDEWLLRELGG"
     gene            complement(1501..1998)
                     /gene="tpx"
                     /locus_tag="PLF56_10855"
     CDS             complement(1501..1998)
                     /gene="tpx"
                     /locus_tag="PLF56_10855"
                     /EC_number="1.11.1.-"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_013160071.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_function: GO:0008379 - thioredoxin peroxidase activity
                     [Evidence IEA];
                     GO_function: GO:0016684 - oxidoreductase activity, acting
                     on peroxide as acceptor [Evidence IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="thiol peroxidase"
                     /protein_id="HOQ54097.1"
                     /translation="MATTNLRGTVVSTVGEPPAVGEPLIPFRLTLPDLTEFASAELTG
                     RRVVLNIFPSIDTRVCALSVRRFNELAAGLANTTVVCISHDLPFALGRFCGVEGIEAV
                     VTASAFRSSFGADYGLTMADGAMSGLLARTVIVADADGIVRHVEITETVGQEPDYEAA
                     LAALG"
     gene            2133..>3376
                     /gene="cimA"
                     /locus_tag="PLF56_10860"
     CDS             2133..>3376
                     /gene="cimA"
                     /locus_tag="PLF56_10860"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_016670035.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_function: GO:0043714 - (R)-citramalate synthase
                     activity [Evidence IEA];
                     GO_process: GO:0009097 - isoleucine biosynthetic process
                     [Evidence IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="citramalate synthase"
                     /protein_id="HOQ54098.1"
                     /translation="MTSLPVAPDTFHVFDTTLRDGAQQEGLRLTVPDKLKIARHLDEL
                     GVDFIEGGWPGANPNDTAFFAAAAEGQLELQHAQLVAFGFTRRVGASAADDPLVQALL
                     DARTPAVCIVAKSHDEHVFRALHTTLDENIEMIRDTVTHLVANGRRVFVDCEHFFDGY
                     KSNPAYALECVRTAAEAGAEVVVLCDTNGGMLPSQMGDIVTAAGAIGVDLGVHCHNDT
                     GCAVANTLAAVDAGVMHVQGTVNGHGERTGNANIITLIANLQLKYGWSLVPESSLREA
                     TRISHAISGITNVPPNSRQPYVGISAFAHKAGLHASAIKVDANLYQHIDPTLVGNDMR
                     MLVSDMAGRANIQLKGEELGYDLSDRELAGRITDLVKQREMDGYSYESADASFELLLR
                     AELGIADDFFEVLTWRVFTGHEV"
BASE COUNT          551 a         1211 c         1136 g          478 t
ORIGIN      
        1 agatcccggc gtgcgccggg atgacggggt cacgacggta agggacacgc tgatcaacac
       61 gcaatccgtt ggctccgggg ggtcacgacg ttgagggaca cgctgatcgc tgtgcgatcc
      121 gttggttacg gtgatccacc ccacggcgca gtgctcagcc gccgagttcg cgcagtagcc
      181 actcgtcggt gacatcccag gtgcgggccg tacgcaccgc catcgtgacc gcgagcgagt
      241 tgccgttcac cagcccgcgc gcgaccggca cccgtcccgc ctcttcgcaa gcccgcacca
      301 agccatgggt ggcggcactt cctgctgcgc tgagcagtcc caccgggcgc ccgtccacgc
      361 ggacctcgac gcggctcggc tccgcgggcg tcagccgagc gcctgcccgc acccgatcgg
      421 catccacagg gacggcaacg tccgtggccc ccacagcctc accgaggggc tcgtcgccgg
      481 gcccatcgag ttgcgtgagg tgcggtgctg acgcatcgtc gaggccgtcg gcggtgtgca
      541 gggtgactac cacggaggcc tgcgcccccg agcccagcag cggagccagc aggtcgagcg
      601 cggccttctc gccggtcacc tgcagggttg tcaccggcgg cagcagcacg aacggagcat
      661 ccggcagcgg gccgggcggg ttgatctggt tcggatcggg cagtcgcagc gtgacccggc
      721 acagcagtcg tcccgagcgt cgccgcgcca ccagccgcat gccgacctgc agcagcccac
      781 cggtgcgggc caaccgttgc agcgccggcc ggatctgctc ggcgtcgggc tccgccagtg
      841 acccgatcca tgcgtcgccg acgagcacgt ccacgaagcc ctcgtcgtcg agcagcagtg
      901 cggcggtgag gtcgtccagt gtggtcgccg cctcggtgat cggacgctcc gcgcgcgcga
      961 acacctgcgc cactcccggc agatacacga acacccggtc cgacgacacc gcggtggtcg
     1021 accaacccca cgcctgaacg aggtgcaggt actgcggcgg ggtgggccgg gtcagcccga
     1081 ccggcaccag agtgagcagg ccgtcggcgt tgaggccgac ggcgcccagc gccccgttgt
     1141 tggtgctcag ccgcccgcgg tacgcgcccg tcacccgctc gggcagccgg ccgaccagct
     1201 gatcgcccac gaagacggcc accgcatccg ggtttcccgg gtcgggtcgc aactcgacgc
     1261 gcaccccgcc cgagggcaac cggaccggcg gagcggccag cgaacgttcg accggcagcc
     1321 aagcatcgtc cggatccggc gcaggcgccg cagtcaggct cacctgcgct gcgcgtcgcg
     1381 ttcgctggtc gcgtgcgatc gcacgggcgc gctgccgccg tcctgcatcg atcgtcagca
     1441 tcacggccgc gaacatcgcg agcgcgacca gtaccgggat gacgaggcgc accatcagga
     1501 tcagcccaac gcggcgagcg ctgcctcgta gtcgggttcc tggccgacgg tctcggtgat
     1561 ctcgacgtgc cggacgatgc cgtcggcgtc ggccacgatc acggtgcgcg ccagcagacc
     1621 gctcatcgca ccgtccgcca tggtcagccc gtagtcggcg ccgaacgagc tgcggaacgc
     1681 cgaggcggtc accacggcct caatgccctc gacgccgcag aagcgaccca acgcgaacgg
     1741 caggtcgtgg ctgatgcaca ccacggtggt gttcgccagc cccgcggcca gttcgttgaa
     1801 ccttcggacg ctcagcgcgc agacccgggt gtcgatgctg ggaaagatgt tcagcaccac
     1861 ccggcgtccg gtcagttcgg cggaggcgaa ctcggtcagg tcgggcaggg tgagccgaaa
     1921 gggaatcagc ggctcgccga cggcgggcgg ttcaccgacg gtgctgacga cggtgccgcg
     1981 aaggttggtg gtggccatgg cgcctgtcta cccctcgcag gggccgtttc ggggcggagg
     2041 tctcagtgcc tgagacccct tgccctggat tggacgtggc gggagactcg aaaggctggt
     2101 gaagttcgcg atctgaccta gaacgaggcg caatgacgag tctgcccgtg gcccccgaca
     2161 cgtttcacgt gttcgatacc acccttcgag acggtgccca acaagagggc ctgcggctga
     2221 ccgtgcccga caagctcaag atcgccaggc atctggacga gttgggcgtc gacttcatcg
     2281 aggggggctg gccgggcgcc aaccccaatg acaccgcctt cttcgccgcc gcggccgagg
     2341 gccagcttga actgcagcac gcccaactgg tcgccttcgg gttcacccgc cgggtcggcg
     2401 catcggccgc cgacgatccg ctggtgcagg ccctgctgga cgcccgcacc ccggccgtct
     2461 gcatcgtggc caagagccac gacgaacacg tgttccgcgc cctgcacacc acgctcgacg
     2521 agaacatcga gatgatccgt gacaccgtca cccacctggt cgccaacgga cggcgggtct
     2581 tcgtcgactg cgaacacttc ttcgacggct acaagtccaa cccggcgtac gcgctcgagt
     2641 gcgtccgcac cgctgccgag gcgggcgccg aggtggtcgt gctctgcgac accaacggcg
     2701 gcatgctgcc ctcccagatg ggtgacatcg tgaccgccgc cggcgcgatc ggcgtcgacc
     2761 tgggcgtcca ttgccacaac gacaccggct gtgcggtggc caacaccctg gccgccgtcg
     2821 acgccggcgt gatgcacgtg cagggcaccg tgaacggaca cggcgagcgc accggcaacg
     2881 ccaacatcat caccttgatc gcgaacctgc agctcaagta cggctggtcg ctggtgcctg
     2941 agtcgtccct gcgcgaggcc acccgcatca gccacgccat ctcgggcatc accaacgtgc
     3001 cgccgaacag ccgccagccc tacgtgggta tttcggcatt cgcgcacaag gccgggctgc
     3061 acgcgtccgc gatcaaggtg gacgccaacc tgtaccagca catcgacccg accctggtcg
     3121 gcaacgacat gcgcatgctg gtcagcgaca tggccggccg ggccaacatt cagctcaagg
     3181 gcgaggagct gggctacgac ctgtccgatc gcgagctggc cggacggatc accgacctgg
     3241 tcaagcagcg cgagatggac ggctacagct acgagtccgc cgacgccagc ttcgagttgc
     3301 tgctgcgcgc cgagctgggc atcgccgacg acttcttcga ggtgctcacc tggcgggtgt
     3361 tcaccggcca cgaggt
//
LOCUS       DAOSUG010000351         3341 bp    DNA     linear   ENV 10-JUN-2024
DEFINITION  MAG TPA_asm: Micropruina sp. isolate AMR_MDS_2195
            NODE_11645_length_3341_cov_3.783628, whole genome shotgun sequence.
ACCESSION   DAOSUG010000351 DAOSUG010000000
VERSION     DAOSUG010000351.1
DBLINK      BioProject: PRJNA925477
            BioSample: SAMN32864262
            Sequence Read Archive: SRR4236673
KEYWORDS    WGS; ENV; Metagenome Assembled Genome; MAG; Third Party Data; TPA;
            TPA:assembly.
SOURCE      Micropruina sp. (wastewater metagenome)
  ORGANISM  Micropruina sp.
            Bacteria; Bacillati; Actinomycetota; Actinomycetes;
            Propionibacteriales; Nocardioidaceae; Micropruina.
REFERENCE   1  (bases 1 to 3341)
  AUTHORS   Abdulkadir,N., Saraiva,J.P., Zhang,J., Stolte,S., Gillor,O.,
            Harms,H. and Rocha,U.
  TITLE     Genome-centric analyses of 165 metagenomes show that mobile genetic
            elements are crucial for the transmission of antimicrobial
            resistance genes to pathogens in activated sludge and wastewater
  JOURNAL   Microbiol Spectr 12 (3), e0291823 (2024)
   PUBMED   38289113
REFERENCE   2  (bases 1 to 3341)
  AUTHORS   Abdulkadir,N.
  TITLE     Direct Submission
  JOURNAL   Submitted (24-MAR-2023) Environmental Microbiology, Helmholtz
            Center for Environmental Research -UFZ, Permoserstr 15, Leipzig
            04318, Germany
COMMENT     The annotation was added by the NCBI Prokaryotic Genome Annotation
            Pipeline (PGAP). Information about PGAP can be found here:
            https://www.ncbi.nlm.nih.gov/genome/annotation_prok/
            
            ##Genome-Assembly-Data-START##
            Assembly Method        :: SPAdes v. 3.11.1
            Genome Representation  :: Full
            Expected Final Version :: Yes
            Genome Coverage        :: Not Applicable
            Sequencing Technology  :: Illumina MiSeq
            ##Genome-Assembly-Data-END##
            
            ##Genome-Annotation-Data-START##
            Annotation Provider               :: NCBI
            Annotation Date                   :: 05/04/2023 04:57:10
            Annotation Pipeline               :: NCBI Prokaryotic Genome
                                                 Annotation Pipeline (PGAP)
            Annotation Method                 :: Best-placed reference protein
                                                 set; GeneMarkS-2+
            Annotation Software revision      :: 6.5
            Features Annotated                :: Gene; CDS; rRNA; tRNA; ncRNA
            Genes (total)                     :: 2,776
            CDSs (total)                      :: 2,750
            Genes (coding)                    :: 2,714
            CDSs (with protein)               :: 2,714
            Genes (RNA)                       :: 26
            tRNAs                             :: 24
            ncRNAs                            :: 2
            Pseudo Genes (total)              :: 36
            CDSs (without protein)            :: 36
            Pseudo Genes (ambiguous residues) :: 0 of 36
            Pseudo Genes (frameshifted)       :: 2 of 36
            Pseudo Genes (incomplete)         :: 32 of 36
            Pseudo Genes (internal stop)      :: 2 of 36
            ##Genome-Annotation-Data-END##
FEATURES             Location/Qualifiers
     source          1..3341
                     /organism="Micropruina sp."
                     /mol_type="genomic DNA"
                     /submitter_seqid="NODE_11645_length_3341_cov_3.783628"
                     /isolate="AMR_MDS_2195"
                     /isolation_source="Activated sludge of municipal
                     wastewater treatment plants"
                     /db_xref="taxon:2737536"
                     /environmental_sample
                     /geo_loc_name="Canada"
                     /lat_lon="49.25 N 123.1 W"
                     /collection_date="2015-06-16"
                     /metagenome_source="wastewater metagenome"
                     /note="metagenomic"
     gene            <1..430
                     /locus_tag="PLF56_10865"
     CDS             <1..430
                     /locus_tag="PLF56_10865"
                     /EC_number="2.7.9.1"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_016291309.1"
                     /note="catalyzes the formation of phosphoenolpyruvate from
                     pyruvate; Derived by automated computational analysis
                     using gene prediction method: Protein Homology."
                     /codon_start=2
                     /transl_table=11
                     /product="pyruvate, phosphate dikinase"
                     /protein_id="HOQ54099.1"
                     /translation="GVDLKYLVGTMIEIPRAAITADEIAKEAEFFSFGTNDLTQMTFG
                     FSRDDAGKFLDAYYEKKIYENDPFARLDQTGVGKLVELAVDLGKQTRPDIKLGICGEH
                     GGDPSSIEFCQKVGLNYVSCSPFRVPIARLAAAQSALKNR"
     gene            686..1522
                     /locus_tag="PLF56_10870"
     CDS             686..1522
                     /locus_tag="PLF56_10870"
                     /inference="COORDINATES: protein motif:HMM:NF015995.2"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_component: GO:0043190 - ATP-binding cassette (ABC)
                     transporter complex [Evidence IEA];
                     GO_function: GO:0022857 - transmembrane transporter
                     activity [Evidence IEA];
                     GO_process: GO:0055085 - transmembrane transport [Evidence
                     IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="glycine betaine ABC transporter
                     substrate-binding protein"
                     /protein_id="HOQ54100.1"
                     /translation="MLTLLLALGGCARQAPQSSDGPTTSPTATALVVGATGDETSRVL
                     AELYVQGLAAKGRTARVVEVDDDANSQVSRLMAGEVDLVPAFAWTAAQTLAVDSDDPK
                     TLVSDLAAALDGEVAVVQPSKVDRAWRFVATTPDGSLAALPTNATIAADSRWKDAPDG
                     LSGLEAVYRAKPSVEIVDDATQRLARVKAGAIGVFTGTDPQVADPSVHQLDDPLSMVT
                     SDPQVALVRIEVSTDDTVLDVVQQLHAKLDDAAVIGIRVRAASVGAPTAVGEWLKANP
                     LT"
     gene            complement(1671..3083)
                     /locus_tag="PLF56_10875"
     CDS             complement(1671..3083)
                     /locus_tag="PLF56_10875"
                     /inference="COORDINATES: protein motif:HMM:NF013954.2"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_function: GO:0004040 - amidase activity [Evidence IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="glucosaminidase domain-containing protein"
                     /protein_id="HOQ54101.1"
                     /translation="MTHPSIRIRAALLAVALSMTGVIGSTVGTTTSLAVVDFDGYIAK
                     AAKAAKASKKAYGVPRAVTIAQSILESGWGRSQLTTKYNNYFGIKCSALVSPHQRGCV
                     AMPTYEYVKGKKKKYIQRFRTYSSMEKSFLDHGRLLNYLDRYNPAFKYPNDPDKFIRA
                     VHKAGYATDPKYSDSVIALMKKWNLYRFDVPAKAKDPNPALISTVAPLAQRSEAATGV
                     PSSVTIAQAIYHSGSGKNAQATKAKNYFSLVCGKTKSAASSGCVSIGGTKYRKYSSIA
                     KSVQDQAAVLSTTTRYAKAMKLSDTPKAFLTAIQKAGWSSSKTYASKVYALITKYGLT
                     KYDLLIAGTIKQGASNAKVTALQNLLAKAGIKVKATGYFGTDTVAAVKKYQSKAKLPV
                     TGRADPLTLTRLAPDVRSGAKGTQVTAVQALLVHRKYAVDARGTFGAKTLKSVKALQK
                     KYKLTRDGIVGERTWALLFG"
BASE COUNT          543 a         1046 c         1132 g          620 t
ORIGIN      
        1 cggcgtcgac ctgaagtacc tggtcggcac catgatcgag atcccgcggg cggcgatcac
       61 cgccgacgag atcgccaaag aggccgagtt cttcagcttc ggcaccaacg acctgacgca
      121 gatgaccttc ggcttcagcc gtgacgacgc gggcaagttc ctggatgcct actacgagaa
      181 gaagatctac gagaacgacc cgttcgcccg gctggatcag accggcgtgg gcaagctggt
      241 cgagctggcg gtcgatctcg gcaagcagac ccggcccgac atcaagctcg gcatctgtgg
      301 cgagcacggt ggtgacccga gttccatcga gttctgccag aaggtcggcc tgaactacgt
      361 cagctgctcg ccgttccggg tgccgatcgc ccggctcgcc gcagcacagt cggcgctgaa
      421 gaaccgctga cgctctgcat cgcacgtggt cctggggccc ggttccctcg tggaatcggg
      481 ccccaggtgg gtagtggggg accttgatcc agtagagccg cgggatccga agctcagggg
      541 gaggcgcgca tcccccttgg gtagcgggtg gtttgctacc gactgcggta gcaagactca
      601 cccgttctgc tgctgcgggt ggcgctgccc gatccgcacc gtccgcgatg gcacagtgga
      661 gcgagtgaca aggtgcctgg tgttgatgct gacgctcctg ttggccctcg gcggttgcgc
      721 ccgtcaggcg ccgcagtcgt ccgatggacc tacgacgagc cccaccgcga cggcactggt
      781 cgtcggggcg accggcgacg agaccagccg ggtgctcgcc gaactctacg tacagggtct
      841 ggccgccaag ggccgaaccg cgcgcgtcgt cgaggtcgac gacgacgcga acagccaggt
      901 gagccgactg atggccgggg aggtcgatct ggtgcccgcc ttcgcctgga cggcggcgca
      961 aacgctcgca gtcgactcgg acgatccaaa gaccctggtc tccgacctgg ccgccgctct
     1021 ggacggcgag gtggcggtgg tgcagccgtc caaggtcgac cgcgcctggc ggttcgttgc
     1081 gaccaccccg gacgggtcgc tggccgcgct gcccacgaac gccacgatcg ccgccgacag
     1141 ccggtggaag gacgccccgg acggcctgtc cggcctcgaa gccgtctatc gggccaagcc
     1201 gagcgtcgag atcgtcgacg atgccacgca gcggctggcg cgggtcaagg cgggggcgat
     1261 cggcgtgttc accggcaccg atccgcaggt cgcggatccg tccgtgcatc aactggacga
     1321 tccgctgtcg atggtcacgt ccgacccgca ggtggcgctg gtgcgaatcg aggtgtccac
     1381 ggacgacacc gtgctcgacg tggttcaaca gttgcacgcc aagctcgacg atgcggccgt
     1441 catcggcatc agggtgcggg ccgcatccgt cggcgcaccg accgcggtgg gggagtggct
     1501 gaaggcaaac ccgctcacct gaccaggcgt cgaacgctcg tagacgcgac ggtgccaagc
     1561 cgacctcgta ccccacctgt gtggacagaa cacctagtca tcagggaaat gctccccgag
     1621 gagacggctg ctagacctgc ggtggatcag ggtctggtca catacgtcgc tcagccgaac
     1681 agcaacgccc aggtgcgctc gccgacaatt ccgtcccggg tcagcttgta cttcttctgc
     1741 agggccttga ccgacttcag ggtcttggcg ccgaaggtgc cgcgtgcgtc cacggcgtat
     1801 ttgcggtgga cgagcagcgc ctgcacggcc gtcacctgcg tgcccttggc gcccgagcgc
     1861 acgtcggggg cgagtctggt cagggtgagg gggtccgcgc gtccggtgac cgggagtttg
     1921 gccttggact gatacttctt gaccgccgcg acggtgtccg tgccgaagta gcccgtggcc
     1981 ttcaccttga tgcccgcttt ggccagcagg ttctgcaggg cggtcacctt ggcattcgac
     2041 gcgccctgtt tgatggtgcc cgcgatcagc aggtcgtatt tggtcaagcc atatttggtg
     2101 atcagcgcgt aaaccttgct ggcgtaggtc ttgctcgacg accaacctgc cttctgaatc
     2161 gccgtcagaa acgccttcgg ggtatcggag agcttcatgg ccttcgcgta ccgcgtcgtg
     2221 gtggacaaaa cggcggcctg gtcctgcacc gacttcgcga tcgagctgta ctttcgatac
     2281 ttcgtgccac cgatgctcac gcacccggac gaggcggcac tcttggtctt gccgcacacc
     2341 aggctgaagt agttcttggc cttcgtcgcc tgagcgttct tgcccgagcc ggagtggtag
     2401 atcgcctggg cgatggtcac cgacgagggg acgccggttg cggcctcgct gcgctgggca
     2461 agcggtgcga cggtcgagat cagggccggg ttggggtcct tggccttggc cggcacgtcg
     2521 aagcggtaca ggttccactt cttcatcaag gcgatgacgc tgtcggagta cttcggatcc
     2581 gtcgcgtaac cggccttgtg cacggcgcgg atgaacttgt ccgggtcgtt ggggtatttg
     2641 aaggcggggt tgtagcgatc caggtagttc aacagacgtc cgtgatcgag aaaggacttc
     2701 tccatcgagc tgtaggtgcg aaaccgctgg atgtacttct tcttcttgcc cttgacgtat
     2761 tcatacgtgg gcatcgccac gcaacccctc tggtgtgggc tgaccagcgc actgcatttg
     2821 atgccgaagt agttgttgta cttcgtggtg agctgggacc ggccccaacc cgactcgagg
     2881 atcgattgcg cgatggtgac ggcgcgtggc accccatagg ccttcttgga cgccttcgcc
     2941 gccttcgccg ccttggcgat gtagccgtcg aagtcgacca ccgccaggct cgtcgtggtg
     3001 cccaccgtgc tgccgatcac ccccgtcatg gacagggcga cggccagcag ggctgcgcga
     3061 atgcgaatgc tcgggtgggt catgggggga ttgaaacctg agaagtcccc gagagccacc
     3121 tgaaccctcg accgttcgtc gacggcttca cccgcgaatc gcatctgact aggtgtaatg
     3181 ctgctcagca accctcaacc aggcatccac cgttgaatgg acgggcgcgg cgtggcggtc
     3241 atcccgggct tgtgccggga tctgggggta cgtggagatc ccggcgtgcg actctcctat
     3301 gtttcgtcaa ggggtgtttg ggtgtaggcg cgggtgaggt g
//
LOCUS       DAOSUG010000352         3334 bp    DNA     linear   ENV 10-JUN-2024
DEFINITION  MAG TPA_asm: Micropruina sp. isolate AMR_MDS_2195
            NODE_11679_length_3334_cov_3.854224, whole genome shotgun sequence.
ACCESSION   DAOSUG010000352 DAOSUG010000000
VERSION     DAOSUG010000352.1
DBLINK      BioProject: PRJNA925477
            BioSample: SAMN32864262
            Sequence Read Archive: SRR4236673
KEYWORDS    WGS; ENV; Metagenome Assembled Genome; MAG; Third Party Data; TPA;
            TPA:assembly.
SOURCE      Micropruina sp. (wastewater metagenome)
  ORGANISM  Micropruina sp.
            Bacteria; Bacillati; Actinomycetota; Actinomycetes;
            Propionibacteriales; Nocardioidaceae; Micropruina.
REFERENCE   1  (bases 1 to 3334)
  AUTHORS   Abdulkadir,N., Saraiva,J.P., Zhang,J., Stolte,S., Gillor,O.,
            Harms,H. and Rocha,U.
  TITLE     Genome-centric analyses of 165 metagenomes show that mobile genetic
            elements are crucial for the transmission of antimicrobial
            resistance genes to pathogens in activated sludge and wastewater
  JOURNAL   Microbiol Spectr 12 (3), e0291823 (2024)
   PUBMED   38289113
REFERENCE   2  (bases 1 to 3334)
  AUTHORS   Abdulkadir,N.
  TITLE     Direct Submission
  JOURNAL   Submitted (24-MAR-2023) Environmental Microbiology, Helmholtz
            Center for Environmental Research -UFZ, Permoserstr 15, Leipzig
            04318, Germany
COMMENT     The annotation was added by the NCBI Prokaryotic Genome Annotation
            Pipeline (PGAP). Information about PGAP can be found here:
            https://www.ncbi.nlm.nih.gov/genome/annotation_prok/
            
            ##Genome-Assembly-Data-START##
            Assembly Method        :: SPAdes v. 3.11.1
            Genome Representation  :: Full
            Expected Final Version :: Yes
            Genome Coverage        :: Not Applicable
            Sequencing Technology  :: Illumina MiSeq
            ##Genome-Assembly-Data-END##
            
            ##Genome-Annotation-Data-START##
            Annotation Provider               :: NCBI
            Annotation Date                   :: 05/04/2023 04:57:10
            Annotation Pipeline               :: NCBI Prokaryotic Genome
                                                 Annotation Pipeline (PGAP)
            Annotation Method                 :: Best-placed reference protein
                                                 set; GeneMarkS-2+
            Annotation Software revision      :: 6.5
            Features Annotated                :: Gene; CDS; rRNA; tRNA; ncRNA
            Genes (total)                     :: 2,776
            CDSs (total)                      :: 2,750
            Genes (coding)                    :: 2,714
            CDSs (with protein)               :: 2,714
            Genes (RNA)                       :: 26
            tRNAs                             :: 24
            ncRNAs                            :: 2
            Pseudo Genes (total)              :: 36
            CDSs (without protein)            :: 36
            Pseudo Genes (ambiguous residues) :: 0 of 36
            Pseudo Genes (frameshifted)       :: 2 of 36
            Pseudo Genes (incomplete)         :: 32 of 36
            Pseudo Genes (internal stop)      :: 2 of 36
            ##Genome-Annotation-Data-END##
FEATURES             Location/Qualifiers
     source          1..3334
                     /organism="Micropruina sp."
                     /mol_type="genomic DNA"
                     /submitter_seqid="NODE_11679_length_3334_cov_3.854224"
                     /isolate="AMR_MDS_2195"
                     /isolation_source="Activated sludge of municipal
                     wastewater treatment plants"
                     /db_xref="taxon:2737536"
                     /environmental_sample
                     /geo_loc_name="Canada"
                     /lat_lon="49.25 N 123.1 W"
                     /collection_date="2015-06-16"
                     /metagenome_source="wastewater metagenome"
                     /note="metagenomic"
     gene            <1..581
                     /locus_tag="PLF56_10880"
     CDS             <1..581
                     /locus_tag="PLF56_10880"
                     /inference="COORDINATES: protein motif:HMM:NF018479.2"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=3
                     /transl_table=11
                     /product="low temperature requirement protein A"
                     /protein_id="HOQ54102.1"
                     /translation="SSLAVTVVVGIVLGESVLASTVAVAGGLQESEHPVRLVVIGLAG
                     FVLAAGMWWLYFATDFGALLPAHRDRSFIWGYGHLLLFPAVAGVGAGLHVAAYVLEHE
                     AHIGTAAAVACVAVPVAGFLLIGFALTIVLTGRPDVPHLALVAAGLAVCAAAVLLAAA
                     GLSMGAALLVVTLSPWVAVVGIELRGRRAAVQ"
     gene            710..1081
                     /locus_tag="PLF56_10885"
     CDS             710..1081
                     /locus_tag="PLF56_10885"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_013861634.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="DUF1992 domain-containing protein"
                     /protein_id="HOQ54103.1"
                     /translation="MQHESCIDRQIREAQERGAFDNLPGAGRPLHLDPDEDWWIKAKL
                     KRENLEAVLPGPLLLRREVEQVQQTLAEVTTEAAAREILEHLNERIRAHYARPSAGPV
                     IVVRLVDVEAELLSWRRARTR"
     assembly_gap    1711..1810
                     /estimated_length=100
                     /gap_type="within scaffold"
                     /linkage_evidence="paired-ends"
     gene            complement(2132..>3334)
                     /locus_tag="PLF56_10890"
     CDS             complement(2132..>3334)
                     /locus_tag="PLF56_10890"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_020392888.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_component: GO:0016020 - membrane [Evidence IEA];
                     GO_function: GO:0004427 - inorganic diphosphate
                     phosphatase activity [Evidence IEA];
                     GO_function: GO:0009678 - pyrophosphate hydrolysis-driven
                     proton transmembrane transporter activity [Evidence IEA];
                     GO_process: GO:1902600 - proton transmembrane transport
                     [Evidence IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="sodium/proton-translocating pyrophosphatase"
                     /protein_id="HOQ54104.1"
                     /translation="FESAVYTAGIIAAAICGVFLISGGSMILSLFMIALAGCGLLTTV
                     GVIVAMDTFGPVSDNAQGIAVMSHDVNEEGEAILTALDADGNTTKAITKGIAIATAVL
                     AATALFGSYTDAVNHALSNLTNLGGSAIVQSMLTYEIVSPITLVGVILGAATVFLFSG
                     LAIDAVTRAAGAIVFEVRRQFAENPGIMTGETRPEYGRVVDICTRDSLRELATPGLLA
                     AFAPIAVGFGLGVGPLAGFLGGAIVAGVLMAVFLANSGGAWDNAKKLVEDGRYGGKHS
                     AAHEATVIGDTVGDPFKDTAGPAINPLIKVMNLVALLIAPAVVALSVGEAANPVVRIA
                     IGTVAALIAMGGVVWSRVRAHKVDLEARLEHEMQLVEELDAAHEQPTSQQVQTDQQQA
                     DEDVQQVR"
BASE COUNT          470 a         1079 c         1174 g          511 t
ORIGIN      
        1 catcctcatt ggctgtaact gtggtggtcg gcatcgtgct gggggagtcg gtgctcgcct
       61 cgacggtggc ggtggccggc gggctgcagg aatccgagca tcccgtgcgg ttggtggtga
      121 tcggtctggc cgggttcgtc ctggccgccg gaatgtggtg gctctacttc gccaccgact
      181 tcggagcgct gctgcccgct catcgcgacc ggtcgttcat ctgggggtac gggcacctgc
      241 tgctgtttcc ggccgtcgcg ggcgtgggcg ccggcctgca cgtggcggcc tacgtgctcg
      301 agcacgaggc gcacatcggc acagccgccg cggtcgcgtg cgtggctgtg cccgtcgccg
      361 ggtttctgct gatcggcttc gcactgacca tcgtgctgac gggacgtccg gacgtgccgc
      421 acctggctct ggtcgcagcc gggctggcgg tctgtgcggc ggctgtgctg ctggcggccg
      481 ccggcctttc gatgggtgcc gcgctgctgg tggtgacgtt gtcaccgtgg gtggcggtgg
      541 tcggcatcga actacggggc cggcgcgcgg ccgtccagta actgcggggc cggcgcgcgg
      601 ccgtccagta actgcgaggc cggcgcgcgg ccgtccagta actgcgaggc cggcgcggcg
      661 ccgtccagta gtggacgggg ccggcgcggc gccgttctac agtcggggca tgcagcacga
      721 gtcgtgcatc gaccggcaga tccgtgaggc gcaggagcgg ggcgccttcg acaacctgcc
      781 gggcgccggc cggccactgc acctcgaccc cgatgaggac tggtggatca aggccaagct
      841 gaagcgcgag aacctcgagg cggtactgcc cggcccgttg ctgctgcggc gtgaggtcga
      901 gcaggtgcag cagacgctcg cagaggtgac caccgaggcc gcggcccgcg agatactcga
      961 gcacctgaac gagcgcatcc gcgcgcacta cgcccgcccg tccgcgggcc cagtcatcgt
     1021 ggtgcgcctg gtcgacgtcg aggccgaact gctgagctgg cgccgggcgc gcacgaggta
     1081 gccgtcgcac gcggcgaccc cccgatcgcc gcgccggtgc ggtcgggctt ggccgaccgg
     1141 gcaggtggtc agtagaggcg tcggtgaacg gcgccaccgc tgccggattg acccgccagt
     1201 gacccgcgca cgccggctga gcgcgcggtc gaagtctgtc gggtcggctc ggcctgtacc
     1261 tgctgaagtg tgccggcgag aatggctgcg cgcacggcgc tcggatcgtt gctgaacatg
     1321 tttcccccct ggatcaatgt caccaactag gaaagcacgc accacccttc cggactaggc
     1381 agaattgcgt aatccgtcaa aaatgttgcc ggaccgtgac cttcaggccc ggtcgcaccg
     1441 ctggtcgagg tcgtcgagac acctgggcac acgggtatcg accagcggcg tactcgtcat
     1501 ttcgcgcttc ccccgggatc tcgcggcacg cagagatccc ggcatccgcc gggatgacgg
     1561 ggatgcgatt ccggcaatgc gccgggatga cgggggtccg ttggttactc gtcatccccg
     1621 ggcttgcccc gggatctcgc ggcacgcaga gatcccggca tccgccggga tgacggggat
     1681 gcgattccgg caatgcgccg ggatgacggg nnnnnnnnnn nnnnnnnnnn nnnnnnnnnn
     1741 nnnnnnnnnn nnnnnnnnnn nnnnnnnnnn nnnnnnnnnn nnnnnnnnnn nnnnnnnnnn
     1801 nnnnnnnnnn ccgggatgac gggatgcggg cgtactcgtc atcccgggct tgccccggga
     1861 tctcgcggca cgagcagatc ccggcatccg ccgggatgac ggggtcacga ttccggcaat
     1921 gcgccgggat gacgggatgc gggcgtactc gtcatcccgg gcttgccccg ggatctcgag
     1981 gcacgagcag atcccggcat gcgccgggat gacgagggtg cgagcgtgcg ccgggatgac
     2041 ggggatgcgg gcgtgcgcca ggatgacggg gccacaatcc cgggcatgcg ccgggatgac
     2101 ggcagggttt cgacgctcga ccgaggaccg atcagcgcac ctgctggacg tcctcgtcgg
     2161 cctgctgctg gtcggtctgc acctgctgcg aggtgggctg ctcgtgtgcc gcgtccaact
     2221 cttcgaccaa ctgcatctcg tgttcgagcc gcgcctcgag atcgaccttg tgcgcccgca
     2281 cccgcgacca caccacgccg cccatggcga tcagcgccgc caccgtgccg atcgcgatgc
     2341 gcaccaccgg gttggcggcc tcgcccaccg acagtgccac cacggctggg gcgatcagca
     2401 gcgcgaccag gttcatcacc ttgatcagcg ggttgatcgc cgggcccgcg gtgtctttga
     2461 acgggtcgcc gaccgtgtca ccgatcaccg tggcctcatg ggccgcggag tgcttgccgc
     2521 cgtagcgtcc gtcttcgacc agcttcttgg cgttgtccca ggcgccgccg gagttggcca
     2581 ggaacaccgc catcagcacc ccggccacga tcgccccgcc gagaaaaccg gccaggggcc
     2641 cgacgccgag gccgaagccg accgcgatcg gcgcgaaggc ggccagcagg cccggcgtgg
     2701 cgagttcgcg caacgaatcg cgggtgcaga tgtcgaccac ccggccgtac tcgggacggg
     2761 tctcgccggt catgatgccc gggttctcgg cgaactggcg ccgcacctcg aacacgatcg
     2821 cgccggcggc ccgggtgacc gcgtcgatgg ccaggccaga gaacaggaac acggttgccg
     2881 cgcccagaat caccccgacc agggtgatcg gcgagacgat ctcgtaggtc agcatcgact
     2941 ggacgatcgc actgccgccc aggttggtca ggttgctgag cgcatggttg accgcatcgg
     3001 tgtacgagcc gaacagcgcg gtggcggcca gcacggccgt ggcgatcgcg atgcccttgg
     3061 tgatcgcctt ggtggtgttg ccgtcggcgt ccagggcggt caggatcgcc tcgccctctt
     3121 cgttcacgtc atggctcatg acggcgatgc cctgggcgtt gtcgctgacc ggtccgaagg
     3181 tgtccatggc gacgatgacg ccgacggtgg tcagcaggcc gcagccggcc agcgcgatca
     3241 tgaacagcga caggatcatc gagccgcccg agatcaggaa cacgccgcag atcgcggcgg
     3301 cgatgatgcc ggcggtgtag acggccgact cgaa
//
LOCUS       DAOSUG010000353         3323 bp    DNA     linear   ENV 10-JUN-2024
DEFINITION  MAG TPA_asm: Micropruina sp. isolate AMR_MDS_2195
            NODE_11723_length_3323_cov_5.420441, whole genome shotgun sequence.
ACCESSION   DAOSUG010000353 DAOSUG010000000
VERSION     DAOSUG010000353.1
DBLINK      BioProject: PRJNA925477
            BioSample: SAMN32864262
            Sequence Read Archive: SRR4236673
KEYWORDS    WGS; ENV; Metagenome Assembled Genome; MAG; Third Party Data; TPA;
            TPA:assembly.
SOURCE      Micropruina sp. (wastewater metagenome)
  ORGANISM  Micropruina sp.
            Bacteria; Bacillati; Actinomycetota; Actinomycetes;
            Propionibacteriales; Nocardioidaceae; Micropruina.
REFERENCE   1  (bases 1 to 3323)
  AUTHORS   Abdulkadir,N., Saraiva,J.P., Zhang,J., Stolte,S., Gillor,O.,
            Harms,H. and Rocha,U.
  TITLE     Genome-centric analyses of 165 metagenomes show that mobile genetic
            elements are crucial for the transmission of antimicrobial
            resistance genes to pathogens in activated sludge and wastewater
  JOURNAL   Microbiol Spectr 12 (3), e0291823 (2024)
   PUBMED   38289113
REFERENCE   2  (bases 1 to 3323)
  AUTHORS   Abdulkadir,N.
  TITLE     Direct Submission
  JOURNAL   Submitted (24-MAR-2023) Environmental Microbiology, Helmholtz
            Center for Environmental Research -UFZ, Permoserstr 15, Leipzig
            04318, Germany
COMMENT     The annotation was added by the NCBI Prokaryotic Genome Annotation
            Pipeline (PGAP). Information about PGAP can be found here:
            https://www.ncbi.nlm.nih.gov/genome/annotation_prok/
            
            ##Genome-Assembly-Data-START##
            Assembly Method        :: SPAdes v. 3.11.1
            Genome Representation  :: Full
            Expected Final Version :: Yes
            Genome Coverage        :: Not Applicable
            Sequencing Technology  :: Illumina MiSeq
            ##Genome-Assembly-Data-END##
            
            ##Genome-Annotation-Data-START##
            Annotation Provider               :: NCBI
            Annotation Date                   :: 05/04/2023 04:57:10
            Annotation Pipeline               :: NCBI Prokaryotic Genome
                                                 Annotation Pipeline (PGAP)
            Annotation Method                 :: Best-placed reference protein
                                                 set; GeneMarkS-2+
            Annotation Software revision      :: 6.5
            Features Annotated                :: Gene; CDS; rRNA; tRNA; ncRNA
            Genes (total)                     :: 2,776
            CDSs (total)                      :: 2,750
            Genes (coding)                    :: 2,714
            CDSs (with protein)               :: 2,714
            Genes (RNA)                       :: 26
            tRNAs                             :: 24
            ncRNAs                            :: 2
            Pseudo Genes (total)              :: 36
            CDSs (without protein)            :: 36
            Pseudo Genes (ambiguous residues) :: 0 of 36
            Pseudo Genes (frameshifted)       :: 2 of 36
            Pseudo Genes (incomplete)         :: 32 of 36
            Pseudo Genes (internal stop)      :: 2 of 36
            ##Genome-Annotation-Data-END##
FEATURES             Location/Qualifiers
     source          1..3323
                     /organism="Micropruina sp."
                     /mol_type="genomic DNA"
                     /submitter_seqid="NODE_11723_length_3323_cov_5.420441"
                     /isolate="AMR_MDS_2195"
                     /isolation_source="Activated sludge of municipal
                     wastewater treatment plants"
                     /db_xref="taxon:2737536"
                     /environmental_sample
                     /geo_loc_name="Canada"
                     /lat_lon="49.25 N 123.1 W"
                     /collection_date="2015-06-16"
                     /metagenome_source="wastewater metagenome"
                     /note="metagenomic"
     gene            <1..119
                     /gene="rpmG"
                     /locus_tag="PLF56_10895"
     CDS             <1..119
                     /gene="rpmG"
                     /locus_tag="PLF56_10895"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_012085006.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_component: GO:0000315 - organellar large ribosomal
                     subunit [Evidence IEA];
                     GO_component: GO:0022625 - cytosolic large ribosomal
                     subunit [Evidence IEA];
                     GO_function: GO:0003735 - structural constituent of
                     ribosome [Evidence IEA];
                     GO_process: GO:0006412 - translation [Evidence IEA]"
                     /codon_start=3
                     /transl_table=11
                     /product="50S ribosomal protein L33"
                     /protein_id="HOQ54105.1"
                     /translation="CKERNYITKKNRRNDPDRLELKKFCPKCHLHTAHRETR"
     gene            412..840
                     /locus_tag="PLF56_10900"
     CDS             412..840
                     /locus_tag="PLF56_10900"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_016455330.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="MaoC family dehydratase N-terminal
                     domain-containing protein"
                     /protein_id="HOQ54106.1"
                     /translation="MISESHLGRTYPPTEPYVVSAAKVAEFAAALGDATNPAYQGPQA
                     IAPPTFAVVLSSAAWGAMFDDPELQLSLSRTVHTDQRFVWRRPLRVGDEVTARLRIDK
                     LRARGTSAFITIVVELDAGGERVCDATSTLLHTWPEQGAA"
     gene            837..1256
                     /locus_tag="PLF56_10905"
     CDS             837..1256
                     /locus_tag="PLF56_10905"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_012923738.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="MaoC/PaaZ C-terminal domain-containing protein"
                     /protein_id="HOQ54107.1"
                     /translation="MSSVLAGVEPGTVLPRITEHLTRDTLVRYAGASTDFNPIHWSDR
                     AADALGLPGVIAHGMLTMGTALRVVTDWCDDPTRVLSYYVRFTKPVVVPDTDEGASLD
                     FGGTVTKVENGVATVSVEALFNGSKVLGAATAEVRVD"
     gene            1261..2334
                     /locus_tag="PLF56_10910"
     CDS             1261..2334
                     /locus_tag="PLF56_10910"
                     /EC_number="1.3.1.98"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_013160467.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_function: GO:0008762 - UDP-N-acetylmuramate
                     dehydrogenase activity [Evidence IEA];
                     GO_function: GO:0071949 - FAD binding [Evidence IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="UDP-N-acetylmuramate dehydrogenase"
                     /protein_id="HOQ54108.1"
                     /translation="MVDTERLADHTTLRVGGPARRWVEATTDAEVIEAVRTADAAGEP
                     VLVLGGGSNVLVADDGFDGTVVHVATRSMEVAGGVCRIDDPHGAQCGGAVIQVAAGEN
                     WDAFVAAAIDNEWRGVEALSGIPGSVGATPIQNVGAYGQEVAESIESVRTWDRTTNQF
                     KTFAVSDCGFGYRSSRFKDEPGRYVVLSVTFQFFQGSRSMPVKYAELARRLGIEPGAK
                     APMREVREAVLELRRSKGMVLDAADHDTWSAGSFFTNPLLSADQAARLPEGAPRFVQP
                     DGRVKSSAAWLIEAAGFSKGFGTPPATLSTKHTLALTNRGGATAHDLLRLARQVRDGV
                     RDRFDITLAPEPVLVGCDLGGAA"
     gene            2495..>3323
                     /locus_tag="PLF56_10915"
     CDS             2495..>3323
                     /locus_tag="PLF56_10915"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_016668022.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_function: GO:0030170 - pyridoxal phosphate binding
                     [Evidence IEA];
                     GO_process: GO:0009058 - biosynthetic process [Evidence
                     IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="aminotransferase class I/II-fold pyridoxal
                     phosphate-dependent enzyme"
                     /protein_id="HOQ54109.1"
                     /translation="MSSVFAAVPLAPRDPILGLTEAFVADPNPHKVNLGVGVYQNAEG
                     KLPLLQCVRQAEQRIAENPAPRGYLPIDGLRAYDAAVKELAFGTDPALLERVSTVQAL
                     GGTGALKIGADFLRQFDPGAKVLISDPSWENHQALFTRAGFTVETYRYFDPEVNGVDV
                     EGMLADLAAAPSGTIVVLHACCHNPTGYDLTEQQWTQVLDTVRSAGLIPFLDMAYQGF
                     SESLDADGAVVRRFAQSGLPVLVSTSFSKSLSLYGERVGALSLVCADPDEAARVLSQG
                     "
BASE COUNT          510 a         1174 c         1150 g          489 t
ORIGIN      
        1 agtgcaagga gcgcaactac atcaccaaga agaaccggcg taacgacccg gatcggcttg
       61 agctgaagaa gttctgcccc aagtgccacc tgcacaccgc gcaccgcgaa actcgctgag
      121 cccagcgagc cgagcgaagc gagctgccgc ccgtggcaac gggcggcttt ttcgcgcgtc
      181 cgcaggcgac tgtgtgtcct gagcagggct gcggaggttc tcggcactgg ttcgcgaacg
      241 gttctcggca cgagattcgg ggacgggcgg cagcccggtt tcacaccttc ggctcagcgc
      301 gacctcgtgc acggctccac cccaaccgca ccacccatgt ccagcccgga tccagctcgg
      361 acgcttcgtc cccccgtggt gatcggctcg acccgccgat aacctgtgcg catgatcagc
      421 gagtcacacc tgggccgcac ctacccgccg accgagccct acgtggtcag cgcggccaag
      481 gtggccgagt tcgcagccgc gctcggcgat gccacaaatc cggcgtacca ggggccgcaa
      541 gcaatcgcgc cgccgacctt cgcggtggtg ctcagttcgg cggcctgggg ggcgatgttc
      601 gacgaccccg agttgcagct gtcactgagt cgcaccgttc acaccgacca gcgtttcgtg
      661 tggcgccgcc cgctgcgggt cggcgacgag gtcacggcca ggctgcgcat cgacaagctg
      721 cgcgcgcgcg gcaccagcgc cttcatcacc atcgtggtcg agctggacgc cggcggcgaa
      781 cgggtctgcg acgccacgtc caccctgctg cacacctggc ccgagcaggg ggccgcatga
      841 gttccgtgtt ggccggcgtc gagccgggca cggtgctgcc gcgcatcacc gagcacctca
      901 cccgcgacac actggtgcgc tacgcgggcg cgtccaccga cttcaacccc atccactggt
      961 cggatcgggc cgcggacgcc cttggcctac ccggcgtgat cgcccacggc atgctcacca
     1021 tgggcaccgc gctgcgggtg gtcaccgact ggtgcgacga cccgacgcgg gtgctcagct
     1081 actacgtgcg gttcaccaag ccggtcgtcg ttcccgacac cgacgagggc gcgtcgctcg
     1141 acttcggcgg caccgtcacc aaggtcgaga acggcgttgc gacggtcagc gtcgaagccc
     1201 tgttcaacgg gtccaaggtg ctcggcgccg cgacagcgga ggtgcgggtt gactgacgcg
     1261 atggttgaca ccgagcggct cgccgaccac accacgctgc gcgtcggcgg cccggccagg
     1321 cgctgggtcg aggcgaccac cgatgccgag gtgatcgagg ccgttcgcac ggccgacgcg
     1381 gccggcgagc cggtgctggt actgggcggc ggctccaacg tgctggtcgc cgacgacggc
     1441 ttcgacggca ccgtcgtgca tgtcgcaacc cgcagcatgg aggtcgccgg gggagtgtgc
     1501 cgcatcgacg atccgcacgg ggcgcagtgc ggcggtgcgg tgatccaggt ggccgccggc
     1561 gagaactggg acgccttcgt cgcggcagcg atcgacaacg agtggcgcgg cgtcgaggcg
     1621 ctgtcgggca ttccgggcag cgtgggcgcc accccgatcc agaacgtcgg cgcctacggg
     1681 caggaggtgg ccgagtcgat cgagtcggtg cgcacctggg accgcacgac caaccagttc
     1741 aagacctttg cggtctccga ctgcggcttc ggctatcgca gttcacggtt caaggacgaa
     1801 ccgggccgct acgtggtgct cagcgtcacg ttccagttct ttcagggcag ccgctccatg
     1861 ccggtcaagt acgccgaact cgcccgtcgt ctcggcatcg agcccggcgc caaggccccg
     1921 atgcgtgagg tgcgcgaagc cgtcctcgag ttgcggcgca gcaagggcat ggtgctcgac
     1981 gcggccgacc acgacacgtg gagcgccggc tcgttcttca cgaacccgct gctgagcgcc
     2041 gatcaggcgg cgcgcctgcc cgagggcgcg ccgaggttcg tccagccgga cgggcgggtc
     2101 aagtccagcg cggcctggct gatcgaagcg gccggcttct ccaaaggctt cggcactcca
     2161 cctgcgaccc tctcgaccaa gcacaccctg gccctgacca accgtggcgg ggcgacggcg
     2221 cacgacctgc tgcggctcgc ccggcaggtg cgcgacgggg tgcgcgatcg gttcgacatc
     2281 accctggcgc ccgaaccggt gctggtgggc tgcgacctcg ggggagccgc ataaccctgg
     2341 gcgcgttccg cgagggcgcg cagtgcgtcc gatcgcaacg tggaagacga gtcgaacagc
     2401 ggtgctgtcg ctgagggtga ccacgcggtg agcgggcgta ccgggtgcgc cgcggcgcgg
     2461 ccagaggcct gcgacacgcc ctagactgac cgccatgtca tcggtcttcg cagcagtgcc
     2521 gcttgccccc cgcgacccca ttctgggtct caccgaggcc ttcgtcgccg accccaatcc
     2581 gcacaaggtc aacctggggg tgggcgtcta ccagaacgcc gagggcaagc tgccgctgct
     2641 gcagtgcgtc cggcaggccg agcagcgcat cgccgagaac ccggcgccgc gtggctacct
     2701 gcccatcgac ggactcaggg cgtacgacgc tgcggtcaag gagttggcgt tcggcaccga
     2761 tcccgccctg ctggagcggg tgagcaccgt ccaggcgctc ggcggcaccg gtgcgctcaa
     2821 gatcggggcc gacttcctgc ggcagttcga cccgggcgca aaggtgctga tctcggaccc
     2881 gtcctgggag aatcaccagg ccctgttcac ccgggccggc ttcaccgtcg agacctaccg
     2941 ctacttcgac cccgaggtca acggtgtcga cgtcgagggc atgctcgccg acctggcggc
     3001 ggccccgtcc ggcaccatcg tggtgctgca tgcctgctgc cacaacccca ccgggtacga
     3061 cctgaccgag cagcagtgga cgcaggtgct cgacaccgtg cgcagcgccg ggctgatccc
     3121 gtttctcgac atggcctacc agggcttctc cgaatccctc gatgcggacg gggcggtcgt
     3181 ccgccggttc gcgcagagcg gactgccggt gctggtgtcg acctcgttca gcaagagcct
     3241 cagcctgtac ggcgaacggg tgggggcgct gtcgctggtc tgcgccgacc cggacgaggc
     3301 cgcgcgggtg ctctcgcagg gca
//
LOCUS       DAOSUG010000354         3318 bp    DNA     linear   ENV 10-JUN-2024
DEFINITION  MAG TPA_asm: Micropruina sp. isolate AMR_MDS_2195
            NODE_11753_length_3318_cov_3.770763, whole genome shotgun sequence.
ACCESSION   DAOSUG010000354 DAOSUG010000000
VERSION     DAOSUG010000354.1
DBLINK      BioProject: PRJNA925477
            BioSample: SAMN32864262
            Sequence Read Archive: SRR4236673
KEYWORDS    WGS; ENV; Metagenome Assembled Genome; MAG; Third Party Data; TPA;
            TPA:assembly.
SOURCE      Micropruina sp. (wastewater metagenome)
  ORGANISM  Micropruina sp.
            Bacteria; Bacillati; Actinomycetota; Actinomycetes;
            Propionibacteriales; Nocardioidaceae; Micropruina.
REFERENCE   1  (bases 1 to 3318)
  AUTHORS   Abdulkadir,N., Saraiva,J.P., Zhang,J., Stolte,S., Gillor,O.,
            Harms,H. and Rocha,U.
  TITLE     Genome-centric analyses of 165 metagenomes show that mobile genetic
            elements are crucial for the transmission of antimicrobial
            resistance genes to pathogens in activated sludge and wastewater
  JOURNAL   Microbiol Spectr 12 (3), e0291823 (2024)
   PUBMED   38289113
REFERENCE   2  (bases 1 to 3318)
  AUTHORS   Abdulkadir,N.
  TITLE     Direct Submission
  JOURNAL   Submitted (24-MAR-2023) Environmental Microbiology, Helmholtz
            Center for Environmental Research -UFZ, Permoserstr 15, Leipzig
            04318, Germany
COMMENT     The annotation was added by the NCBI Prokaryotic Genome Annotation
            Pipeline (PGAP). Information about PGAP can be found here:
            https://www.ncbi.nlm.nih.gov/genome/annotation_prok/
            
            ##Genome-Assembly-Data-START##
            Assembly Method        :: SPAdes v. 3.11.1
            Genome Representation  :: Full
            Expected Final Version :: Yes
            Genome Coverage        :: Not Applicable
            Sequencing Technology  :: Illumina MiSeq
            ##Genome-Assembly-Data-END##
            
            ##Genome-Annotation-Data-START##
            Annotation Provider               :: NCBI
            Annotation Date                   :: 05/04/2023 04:57:10
            Annotation Pipeline               :: NCBI Prokaryotic Genome
                                                 Annotation Pipeline (PGAP)
            Annotation Method                 :: Best-placed reference protein
                                                 set; GeneMarkS-2+
            Annotation Software revision      :: 6.5
            Features Annotated                :: Gene; CDS; rRNA; tRNA; ncRNA
            Genes (total)                     :: 2,776
            CDSs (total)                      :: 2,750
            Genes (coding)                    :: 2,714
            CDSs (with protein)               :: 2,714
            Genes (RNA)                       :: 26
            tRNAs                             :: 24
            ncRNAs                            :: 2
            Pseudo Genes (total)              :: 36
            CDSs (without protein)            :: 36
            Pseudo Genes (ambiguous residues) :: 0 of 36
            Pseudo Genes (frameshifted)       :: 2 of 36
            Pseudo Genes (incomplete)         :: 32 of 36
            Pseudo Genes (internal stop)      :: 2 of 36
            ##Genome-Annotation-Data-END##
FEATURES             Location/Qualifiers
     source          1..3318
                     /organism="Micropruina sp."
                     /mol_type="genomic DNA"
                     /submitter_seqid="NODE_11753_length_3318_cov_3.770763"
                     /isolate="AMR_MDS_2195"
                     /isolation_source="Activated sludge of municipal
                     wastewater treatment plants"
                     /db_xref="taxon:2737536"
                     /environmental_sample
                     /geo_loc_name="Canada"
                     /lat_lon="49.25 N 123.1 W"
                     /collection_date="2015-06-16"
                     /metagenome_source="wastewater metagenome"
                     /note="metagenomic"
     gene            complement(29..379)
                     /locus_tag="PLF56_10920"
     CDS             complement(29..379)
                     /locus_tag="PLF56_10920"
                     /inference="COORDINATES: ab initio
                     prediction:GeneMarkS-2+"
                     /note="Derived by automated computational analysis using
                     gene prediction method: GeneMarkS-2+."
                     /codon_start=1
                     /transl_table=11
                     /product="hypothetical protein"
                     /protein_id="HOQ54110.1"
                     /translation="MDESDGEGSLLARPADSVWVGLGSDEVGVGVGVGVGPVLGGGVG
                     LGVFDGVGLGVGFGVLVLGDGLGVGFADFVWDGLGDGVATAFGLGLVVGPRLGIVPLG
                     RGDGLPVRYGDGST"
     gene            399..2705
                     /locus_tag="PLF56_10925"
     CDS             399..2705
                     /locus_tag="PLF56_10925"
                     /inference="COORDINATES: ab initio
                     prediction:GeneMarkS-2+"
                     /note="Derived by automated computational analysis using
                     gene prediction method: GeneMarkS-2+."
                     /codon_start=1
                     /transl_table=11
                     /product="hypothetical protein"
                     /protein_id="HOQ54111.1"
                     /translation="MTGVIAGWRRRRLFASPTRKRRAVPRWAWLPFAVLATLLGLLAA
                     LRALGLDGPDWLLWLTDPRQPVVPAVFLGSIVAAAGLYGRARHVAGRVIGVLSLSAFA
                     VGALVLGLASYVNCAPQGLPFFEPLTWVLSGNGAEPWGAGPGCPTHTPLAAHLARLCT
                     LWGALAGVVGIVVASMRRQIDRWRAFWSRRSVVIMGSAPQVAPVVRVLAAALAPKTAL
                     VLVASGGDETFLANTRTMGGRVLASDTVDAEALTRVLTTRGRICAKSVHVIEERVPEA
                     LAGFARVARALDAAKANEAMVTRVLVRIDDPWAAENWRRATISPQRAWLADALSATEQ
                     TARAIVARIELIGARRLVLAGQGDLAAAVLAEVARTERERRVLTPTQRLDPLEVTLVG
                     TAAAQLAADHSAQQARFGNDSGIDSVRVIDDPASDPVLVVAGRSELTTLLVIADPVPA
                     LNRPTRLAGQLPEVTILAVDPASTEMPEQPLLGRLTSFGVALLSGQDPPEDHWTRMAR
                     LLHTAYLNQLGDAVGSRPSAQPWAELPQFFRDSNLRQVHSLFSGVALLGRTWAAQGTG
                     EQLDPAEIDFLSRREHDSWRRFYLANGWRQAAVRDDSRLYHDWLVEWDKLPPPARVRT
                     TGSVRRALALLDVAGYRSEREARLLPFRRLGEVRATRAERPLTWHTESGATMRAAAGD
                     WLLTDPQTSRQWSADPQAFAAAHEPLGGDRYRRTGFVQARPAHLHEQVATLEGPAVAA
                     PGDWLVEGDLHERWLVPAARFAAAYEAV"
     gene            2822..>3318
                     /locus_tag="PLF56_10930"
     CDS             2822..>3318
                     /locus_tag="PLF56_10930"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_015068807.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="L-lactate dehydrogenase"
                     /protein_id="HOQ54112.1"
                     /translation="MTINTAPTKLSVIGAGAVGSSLAYAALIRGSAQRIALYDINDKK
                     ADAEVRDLNHGTQFTPSSGVEGGGNIEVVKGSDVVIITAGAAQRPGQTRLELAEVNTN
                     ILKSLMPQLLEQAPNALYVLVTNPCDVLTVVAQKVSGLPSTQVFSTGTMLDTSRLRFA
                     IAEVAR"
BASE COUNT          463 a         1207 c         1164 g          484 t
ORIGIN      
        1 tgcacttcga ggtcaaggtc aacggctcct acgtcgaccc gtcgccatac ctgaccggca
       61 agccgtcccc gcgccccagc ggcacgatcc ccaacctggg ccccaccacg agccccagcc
      121 cgaaggccgt cgccacgcca tcgcccagcc cgtcccagac gaagtcggca aagcccacgc
      181 ccagcccgtc cccgagcacc aagacgccga agcccacccc gagccccaca ccgtcgaaga
      241 cgcccaaacc cacgccgccg ccgagcaccg gcccgacgcc gacccccaca ccgaccccca
      301 cctcgtccga gcccagcccg acccagaccg agtcggcggg ccgggccagc aacgacccct
      361 cgccgtcgga ctcgtccacg ccgtaggctc accccctgat gaccggggtg atcgcaggct
      421 ggcggcgccg gcgactgttc gcctcgccca cccgcaagcg tcgcgcggtg ccgcgctggg
      481 cctggctgcc gttcgccgtg ctggccaccc tgctcggcct gctggccgca cttcgtgccc
      541 tgggcctgga cggaccggac tggttgctgt ggttgaccga tccccggcag ccggtggtgc
      601 cggcggtgtt tctcggttcg atcgtcgccg cggccggcct gtacggacgg gcccggcacg
      661 tcgccggccg ggtgatcggg gtgttgagcc tgtcggcctt cgcggtgggt gcactggtgt
      721 tgggactggc gtcgtatgtg aactgtgcgc cccaggggct gccgttcttc gagccactga
      781 cctgggtgtt gtcgggtaac ggtgccgagc cctggggggc cgggcccggt tgccccaccc
      841 acaccccgct ggcggcgcat ctggcccggc tgtgcaccct gtggggtgcg ctggccgggg
      901 tggtcggcat cgtggtggcc agcatgcgcc ggcagatcga ccgttggcgg gcgttctgga
      961 gccggcgttc ggtcgtgatc atggggtccg cgccgcaggt ggcaccggtc gtgcgggtgc
     1021 tcgcggccgc gctggcaccc aagacggccc tggtgctggt cgcctcgggc ggcgacgaga
     1081 cctttctcgc gaacacccgc accatgggtg gccgggtgct ggcctcggac accgtcgatg
     1141 ccgaggcgct cacccgggtg ctgaccaccc gcggacgtat ctgcgccaag tcggtgcacg
     1201 tgatcgaaga gcgggtaccg gaggcgctgg ccggcttcgc ccgggtggcg cgggcactgg
     1261 acgctgcgaa ggccaacgag gcgatggtca cccgggtgct ggtgcgcatc gatgacccgt
     1321 gggcagccga gaactggcgc cgcgccacga tcagcccgca gcgcgcctgg ctggccgacg
     1381 cattgtcggc caccgagcag acggcgcggg cgatcgtggc gcggatcgaa ctgatcgggg
     1441 cgcgtcggct ggtactggcc gggcagggtg acctggcggc cgccgtgctg gccgaggtgg
     1501 cccggaccga acgcgagcgc cgggtgctga cgcccacgca gcgactcgat ccgctcgagg
     1561 tcaccctggt cggcactgca gcggcgcaac tggccgccga tcactccgcg cagcaggccc
     1621 ggttcggcaa cgacagcggc atcgactcgg tgcgcgtgat cgacgatccg gcgtccgatc
     1681 cggtgctggt ggtcgccggg cgcagtgagt tgaccacgct gctggtgatc gccgacccgg
     1741 taccggcact caaccggccc acccggctgg ccggacaact gcctgaggtg acgattctgg
     1801 ccgtcgaccc agcctcgacc gagatgcccg agcagccgtt gctcgggcgg ctgaccagct
     1861 tcggggtcgc cctgctcagc ggccaggatc cgcccgagga tcactggacc cggatggccc
     1921 ggctgctgca caccgcctac ctcaaccaac ttggtgacgc ggtgggcagc cggccctctg
     1981 ctcagccgtg ggccgaactg ccgcagttct tccgcgacag caaccttcgc caggtgcaca
     2041 gcctcttctc gggcgtggcc ctgctcggtc gcacctgggc ggcgcagggc accggcgagc
     2101 aactggaccc ggccgagatc gactttctga gccggcgcga gcacgattcg tggcgtcgct
     2161 tctacctggc caacggctgg cggcaggcgg ccgtccgcga cgattcgagg ctgtaccacg
     2221 actggctggt cgagtgggac aagttgccgc cgcctgcgcg ggtgcgcacc accggcagcg
     2281 tccggcgggc gttggcgctg ctggacgtgg ccggctaccg atccgagcgc gaagccaggc
     2341 tgctgccctt ccgccgattg ggtgaggtgc gggccacccg ggcggagcgg ccgctgacct
     2401 ggcacaccga atccggggcc accatgcggg cggcggccgg cgactggctg ctcaccgatc
     2461 cccagacctc gcggcaatgg tcggccgacc cgcaggcgtt cgccgccgct cacgagccgc
     2521 tgggcggcga ccggtaccgc cgaaccggct tcgtacaggc gcgtccggcc cacctgcacg
     2581 agcaggtggc gaccctggag ggcccggcgg tggccgcacc gggggactgg ctggtcgagg
     2641 gcgacctgca cgagcgctgg ctggtgcctg cggcccggtt cgcagctgcg tacgaggcgg
     2701 tgtgaggcga cgacctgtcg tagtctgtca agactcgagc ggcgcggttg cccgagtctt
     2761 ggtgcatgac attgttcgcc caccgctgcc atcagtacgg gaggaacgct agcgtggagc
     2821 catgacgatc aacactgccc ccaccaaact ctcggtcatc ggcgccggtg ccgtcggctc
     2881 gtccctggcc tacgccgcgt tgatccgtgg ctcggctcaa cggatcgccc tgtacgacat
     2941 caatgacaag aaggccgacg ccgaggtgcg cgacctcaac cacggcaccc agttcacccc
     3001 ttcgtccggt gtcgagggcg gtggcaacat cgaggtggtc aagggttccg acgtggtgat
     3061 catcaccgcc ggcgcagccc agcggcccgg ccagacccgg ctcgagctgg ccgaggtcaa
     3121 caccaacatc ctgaagtccc tgatgccgca gctgctcgag caggccccga acgcgctcta
     3181 cgtgctggtc accaacccct gtgacgtgtt gaccgtggtg gcgcagaagg tgagcgggct
     3241 gcccagcacg caggtgttct cgaccggcac catgctcgac acctcgcgac tgcggttcgc
     3301 gatcgccgag gtggctcg
//
LOCUS       DAOSUG010000355         3312 bp    DNA     linear   ENV 10-JUN-2024
DEFINITION  MAG TPA_asm: Micropruina sp. isolate AMR_MDS_2195
            NODE_11784_length_3312_cov_4.340190, whole genome shotgun sequence.
ACCESSION   DAOSUG010000355 DAOSUG010000000
VERSION     DAOSUG010000355.1
DBLINK      BioProject: PRJNA925477
            BioSample: SAMN32864262
            Sequence Read Archive: SRR4236673
KEYWORDS    WGS; ENV; Metagenome Assembled Genome; MAG; Third Party Data; TPA;
            TPA:assembly.
SOURCE      Micropruina sp. (wastewater metagenome)
  ORGANISM  Micropruina sp.
            Bacteria; Bacillati; Actinomycetota; Actinomycetes;
            Propionibacteriales; Nocardioidaceae; Micropruina.
REFERENCE   1  (bases 1 to 3312)
  AUTHORS   Abdulkadir,N., Saraiva,J.P., Zhang,J., Stolte,S., Gillor,O.,
            Harms,H. and Rocha,U.
  TITLE     Genome-centric analyses of 165 metagenomes show that mobile genetic
            elements are crucial for the transmission of antimicrobial
            resistance genes to pathogens in activated sludge and wastewater
  JOURNAL   Microbiol Spectr 12 (3), e0291823 (2024)
   PUBMED   38289113
REFERENCE   2  (bases 1 to 3312)
  AUTHORS   Abdulkadir,N.
  TITLE     Direct Submission
  JOURNAL   Submitted (24-MAR-2023) Environmental Microbiology, Helmholtz
            Center for Environmental Research -UFZ, Permoserstr 15, Leipzig
            04318, Germany
COMMENT     The annotation was added by the NCBI Prokaryotic Genome Annotation
            Pipeline (PGAP). Information about PGAP can be found here:
            https://www.ncbi.nlm.nih.gov/genome/annotation_prok/
            
            ##Genome-Assembly-Data-START##
            Assembly Method        :: SPAdes v. 3.11.1
            Genome Representation  :: Full
            Expected Final Version :: Yes
            Genome Coverage        :: Not Applicable
            Sequencing Technology  :: Illumina MiSeq
            ##Genome-Assembly-Data-END##
            
            ##Genome-Annotation-Data-START##
            Annotation Provider               :: NCBI
            Annotation Date                   :: 05/04/2023 04:57:10
            Annotation Pipeline               :: NCBI Prokaryotic Genome
                                                 Annotation Pipeline (PGAP)
            Annotation Method                 :: Best-placed reference protein
                                                 set; GeneMarkS-2+
            Annotation Software revision      :: 6.5
            Features Annotated                :: Gene; CDS; rRNA; tRNA; ncRNA
            Genes (total)                     :: 2,776
            CDSs (total)                      :: 2,750
            Genes (coding)                    :: 2,714
            CDSs (with protein)               :: 2,714
            Genes (RNA)                       :: 26
            tRNAs                             :: 24
            ncRNAs                            :: 2
            Pseudo Genes (total)              :: 36
            CDSs (without protein)            :: 36
            Pseudo Genes (ambiguous residues) :: 0 of 36
            Pseudo Genes (frameshifted)       :: 2 of 36
            Pseudo Genes (incomplete)         :: 32 of 36
            Pseudo Genes (internal stop)      :: 2 of 36
            ##Genome-Annotation-Data-END##
FEATURES             Location/Qualifiers
     source          1..3312
                     /organism="Micropruina sp."
                     /mol_type="genomic DNA"
                     /submitter_seqid="NODE_11784_length_3312_cov_4.340190"
                     /isolate="AMR_MDS_2195"
                     /isolation_source="Activated sludge of municipal
                     wastewater treatment plants"
                     /db_xref="taxon:2737536"
                     /environmental_sample
                     /geo_loc_name="Canada"
                     /lat_lon="49.25 N 123.1 W"
                     /collection_date="2015-06-16"
                     /metagenome_source="wastewater metagenome"
                     /note="metagenomic"
     gene            complement(<1..563)
                     /locus_tag="PLF56_10935"
     CDS             complement(<1..563)
                     /locus_tag="PLF56_10935"
                     /inference="COORDINATES: ab initio
                     prediction:GeneMarkS-2+"
                     /note="Derived by automated computational analysis using
                     gene prediction method: GeneMarkS-2+."
                     /codon_start=1
                     /transl_table=11
                     /product="hypothetical protein"
                     /protein_id="HOQ54113.1"
                     /translation="MRRIHALALVPVFALAACTTATAPATTAAPATSAPATSAPATST
                     PVDAGATLSGDGYTLTLPKGWEDATAAFKKLQAQVDTGAKETADTKDKFNDNVNVVVQ
                     TSAEVPFESLKTALKTQLEQAGSTNIEFKDNATLDGKDALHVWSITKDAGDAHTIQFM
                     AFNGGKLYVLTVSTNRDDAAAATLSQQ"
     gene            617..1024
                     /locus_tag="PLF56_10940"
     CDS             617..1024
                     /locus_tag="PLF56_10940"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_013866140.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="hypothetical protein"
                     /protein_id="HOQ54114.1"
                     /translation="MSSSSRAEDARRREELAAAAQRNEAAAAQVLVDEFVATAKQRGL
                     VPEPLRAHLMDGTRVKSDQVGWYLNKARTLAIAPDGKFYQLLTTGGWLTRFTGVKLTP
                     SLPPLVVGRGGRDGETGDLSDFLARALEDYTPL"
     gene            1607..2257
                     /locus_tag="PLF56_10945"
     CDS             1607..2257
                     /locus_tag="PLF56_10945"
                     /inference="COORDINATES: ab initio
                     prediction:GeneMarkS-2+"
                     /note="Derived by automated computational analysis using
                     gene prediction method: GeneMarkS-2+."
                     /codon_start=1
                     /transl_table=11
                     /product="hypothetical protein"
                     /protein_id="HOQ54115.1"
                     /translation="MSRAADPSIDALNREWTQLQSLTVPARWPVQELTLADLLSAVGD
                     DPDRVLGTLLRVHADGDALAGRVVLQAMLGKLVLLAVHDPYHAAAEYIAECWLQLCQY
                     PLARRPARIAANLALDTRRAVWASPTPAVSTDPLAFDEMAQPGSVDTIALIRTATRLG
                     LIDHASGACLVAVYCLGLRSHEAAAYLAISPQLVRWRNARSIRRLAPHAVTLAAVA"
     gene            2416..>2739
                     /locus_tag="PLF56_10950"
     CDS             2416..>2739
                     /locus_tag="PLF56_10950"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_013863407.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="hypothetical protein"
                     /protein_id="HOQ54116.1"
                     /translation="MPQAQPGTRRLRIGCEFNHVAQVPTPAAFLIRPAADAPIVCESW
                     TTDPVLEYGHYNDLYGNEVRRVILPAGESLVSYYAIANVPDEVDEADFSAPALRRGAC
                     EEELRI"
     assembly_gap    2740..2749
                     /estimated_length=10
                     /gap_type="within scaffold"
                     /linkage_evidence="paired-ends"
     gene            <2750..>3312
                     /locus_tag="PLF56_10955"
     CDS             <2750..>3312
                     /locus_tag="PLF56_10955"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_013863407.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="transglutaminase family protein"
                     /protein_id="HOQ54117.1"
                     /translation="PALLAEELPHDALVYLLPSRYCQSDMLSGPAWDLFGSRERTYEL
                     VAKIQDWVWNHIEYRTGSTGSWWTAKDSFDNGYGVCRDFAHLMVTMCRAVTIPARYCY
                     GYLPDMDVEPLPTAMDFHAWVEVYLGDRWYTFDPRHNARRKGHVPIARGRDATDLAMV
                     TTWGVPWLRRLTVWADEDFSEPWPPLD"
BASE COUNT          526 a         1054 c         1147 g          575 t
ORIGIN      
        1 atctgctgcg agagggtggc cgcagcggcg tcgtcgcggt tggtcgagac ggtgagcacg
       61 tacagcttgc cgccattgaa cgccatgaac tgaatggtgt gggcatcgcc cgcgtccttg
      121 gtgatcgacc agacgtgcaa cgcgtccttg ccgtcgagtg tggcgttgtc cttgaactcg
      181 atgttcgtcg agccggcctg ctcgagttgg gtcttgagcg cggtcttgag cgactcgaat
      241 gggacctcgg cggaggtctg caccacgacg ttcacgttgt cgttgaactt gtccttggtg
      301 tcggcggtct ctttggcgcc ggtgtcgacc tgggcctgaa gtttcttgaa ggcggcggtg
      361 gcgtcctccc agcccttcgg cagcgtcaag gtgtagccgt cgccgctcag cgtcgcgccc
      421 gcatcgacgg gggtggacgt ggccggcgcg gaggtggcgg gggcggaggt ggccggtgcg
      481 gcagtggtgg caggcgcggt cgccgtggtg caggcggcga gggcgaaaac gggaacgagg
      541 gcgagtgcat ggattcggcg catgcgcacg aagtgtaacc gtgaagaggt catgccctcg
      601 gggtagggtg tgcggcatgt cgtccagcag ccgggccgaa gacgcccgcc ggcgcgaaga
      661 gttggctgct gccgctcagc gcaatgaggc ggccgccgcg caggtgctgg tcgacgagtt
      721 cgtggccacc gccaagcagc gtggcctggt gccggagccg ttgcgggccc acctgatgga
      781 cggtacccgg gtcaagagcg accaggtcgg ctggtatctg aacaaggcac gcacgcttgc
      841 gatcgcaccc gatggcaagt tctatcagct gctcaccacc ggcggatggc tgacgcgctt
      901 caccggcgtg aagctgacgc ccagcctgcc gcccctggtg gtgggccgtg gtggacgcga
      961 cggtgagacg ggtgacctct cggactttct ggctcgggcg ttggaggact acacacccct
     1021 ctgacggtgg tcgtcgccga tcactgcccg acgtcaggta ccgacgagtg cgtcgttcgg
     1081 ctctggcgac cggtcagtca tggcgttggc caacgatccg cgtgtgtccg gacgatcgcc
     1141 acgtgcgaaa ctgcaccaga tctggcattg gctccgcctc agtgaggcgg cagccccaca
     1201 caatgtggtg cagtttcgca cgcgtcaagc ctgacgctga ggtgccaggt gagccccggt
     1261 gtggacccgc tcgagcaaca tgtgcggggg gcgacccgaa gccagggctg agccggcccg
     1321 gtagtaaggg ctccaggggg ctgcaggacc gtccaggcag ccccctgggc gaagtggatg
     1381 gctgagccgg caaccaagaa cccgctagtt ggtgacgtat caattgtatg gctttcgcag
     1441 gctccgcgcg cactgtgagc gcactgtcgc gcgaacagtt atcagatcgt ggccccgcgt
     1501 gacgaacacc gtccctttcg aacggacaaa caaccggcaa ggcggtgaca acaccgcctg
     1561 gactgggaca acacccctga caacacctga tcggcagcgt gggggcatgt ccagagcagc
     1621 agacccgagc atcgacgcgc tgaatcgtga atggacccaa ctccagtcgc tgaccgtgcc
     1681 ggctcggtgg ccggttcagg agctcaccct cgccgatctg ttgagtgccg tcggtgacga
     1741 tccggatcgg gtgctcggca cattactgcg tgttcatgcc gacggcgatg cgctcgccgg
     1801 acgggtggtg ctgcaggcga tgctgggcaa gctggtgttg ctggctgtgc acgatccgta
     1861 ccatgcagcg gccgagtaca tcgcggaatg ctggcttcaa ctgtgccagt acccgctcgc
     1921 ccgccgtccg gcacgcattg ccgccaacct cgcgctcgac acccggcgtg cggtgtgggc
     1981 gagccccaca cctgcggtga gcaccgaccc gctggcattc gatgagatgg cgcaacccgg
     2041 ttcagtcgac accatcgcgc tgattcggac ggccacgcgg ctgggcctca tagaccacgc
     2101 gtccggcgcc tgcctggtcg cggtgtactg cctgggcctg cgcagtcacg aagccgctgc
     2161 ctacctcgcc atcagtccgc agttggtgcg ctggcgcaac gcccggtcga tccggcggct
     2221 ggctccccac gccgtcaccc tcgctgcggt ggcctgatgt gcccgcgact ctcggccaat
     2281 cgagtgcttt ctagtgcgaa ccctagataa tcaaagaagg tcgcatacct accttcaggt
     2341 gaggcgacgc gggcactgcg tgggcggtgt gtgccggtgg tataagttgg cgctctcatt
     2401 cagaagggag ccctggtgcc gcaggctcaa cccggtactc gcaggctgcg catcggctgc
     2461 gagttcaatc acgtcgcgca ggtccccacg cccgccgcct tcctgatccg tccggccgcc
     2521 gacgccccga tcgtgtgcga gtcctggacc accgatcccg tgctcgagta cgggcattac
     2581 aacgaccttt acggcaacga ggtgcgtcgg gtgatcctgc cggccggcga atccctggtg
     2641 tcgtactacg cgatcgcgaa cgtgccggac gaggtcgacg aggccgactt cagcgccccc
     2701 gcgctgcggc gcggcgcctg cgaggaagag ctgcgcatcn nnnnnnnnnc ccgcgctgct
     2761 ggccgaagag ctgccgcacg acgcgctggt gtacctgctg cccagtcggt actgccaatc
     2821 cgacatgttg agcgggccgg cctgggacct gttcggctcg cgggagcgca cctacgagtt
     2881 ggtggccaag atccaggact gggtctggaa ccacatcgag tatcgaacgg gcagcacggg
     2941 ctcgtggtgg accgcgaagg actctttcga caacggctac ggggtgtgcc gagacttcgc
     3001 gcacctgatg gtgaccatgt gccgcgcggt caccatcccg gcccgctact gctacgggta
     3061 tctgcccgac atggacgtgg agccgctgcc caccgccatg gactttcacg cgtgggtcga
     3121 ggtctacctg ggcgatcgct ggtacacgtt cgacccccgg cacaacgccc ggcgcaaggg
     3181 tcacgtgccg atcgcacgcg ggcgtgatgc gaccgacctg gcgatggtca ccacctgggg
     3241 cgtgccgtgg ctgcgtcgac tgacggtgtg ggccgacgag gacttcagcg aaccgtggcc
     3301 gccgctggac at
//
LOCUS       DAOSUG010000356         3309 bp    DNA     linear   ENV 10-JUN-2024
DEFINITION  MAG TPA_asm: Micropruina sp. isolate AMR_MDS_2195
            NODE_11807_length_3309_cov_4.172711, whole genome shotgun sequence.
ACCESSION   DAOSUG010000356 DAOSUG010000000
VERSION     DAOSUG010000356.1
DBLINK      BioProject: PRJNA925477
            BioSample: SAMN32864262
            Sequence Read Archive: SRR4236673
KEYWORDS    WGS; ENV; Metagenome Assembled Genome; MAG; Third Party Data; TPA;
            TPA:assembly.
SOURCE      Micropruina sp. (wastewater metagenome)
  ORGANISM  Micropruina sp.
            Bacteria; Bacillati; Actinomycetota; Actinomycetes;
            Propionibacteriales; Nocardioidaceae; Micropruina.
REFERENCE   1  (bases 1 to 3309)
  AUTHORS   Abdulkadir,N., Saraiva,J.P., Zhang,J., Stolte,S., Gillor,O.,
            Harms,H. and Rocha,U.
  TITLE     Genome-centric analyses of 165 metagenomes show that mobile genetic
            elements are crucial for the transmission of antimicrobial
            resistance genes to pathogens in activated sludge and wastewater
  JOURNAL   Microbiol Spectr 12 (3), e0291823 (2024)
   PUBMED   38289113
REFERENCE   2  (bases 1 to 3309)
  AUTHORS   Abdulkadir,N.
  TITLE     Direct Submission
  JOURNAL   Submitted (24-MAR-2023) Environmental Microbiology, Helmholtz
            Center for Environmental Research -UFZ, Permoserstr 15, Leipzig
            04318, Germany
COMMENT     The annotation was added by the NCBI Prokaryotic Genome Annotation
            Pipeline (PGAP). Information about PGAP can be found here:
            https://www.ncbi.nlm.nih.gov/genome/annotation_prok/
            
            ##Genome-Assembly-Data-START##
            Assembly Method        :: SPAdes v. 3.11.1
            Genome Representation  :: Full
            Expected Final Version :: Yes
            Genome Coverage        :: Not Applicable
            Sequencing Technology  :: Illumina MiSeq
            ##Genome-Assembly-Data-END##
            
            ##Genome-Annotation-Data-START##
            Annotation Provider               :: NCBI
            Annotation Date                   :: 05/04/2023 04:57:10
            Annotation Pipeline               :: NCBI Prokaryotic Genome
                                                 Annotation Pipeline (PGAP)
            Annotation Method                 :: Best-placed reference protein
                                                 set; GeneMarkS-2+
            Annotation Software revision      :: 6.5
            Features Annotated                :: Gene; CDS; rRNA; tRNA; ncRNA
            Genes (total)                     :: 2,776
            CDSs (total)                      :: 2,750
            Genes (coding)                    :: 2,714
            CDSs (with protein)               :: 2,714
            Genes (RNA)                       :: 26
            tRNAs                             :: 24
            ncRNAs                            :: 2
            Pseudo Genes (total)              :: 36
            CDSs (without protein)            :: 36
            Pseudo Genes (ambiguous residues) :: 0 of 36
            Pseudo Genes (frameshifted)       :: 2 of 36
            Pseudo Genes (incomplete)         :: 32 of 36
            Pseudo Genes (internal stop)      :: 2 of 36
            ##Genome-Annotation-Data-END##
FEATURES             Location/Qualifiers
     source          1..3309
                     /organism="Micropruina sp."
                     /mol_type="genomic DNA"
                     /submitter_seqid="NODE_11807_length_3309_cov_4.172711"
                     /isolate="AMR_MDS_2195"
                     /isolation_source="Activated sludge of municipal
                     wastewater treatment plants"
                     /db_xref="taxon:2737536"
                     /environmental_sample
                     /geo_loc_name="Canada"
                     /lat_lon="49.25 N 123.1 W"
                     /collection_date="2015-06-16"
                     /metagenome_source="wastewater metagenome"
                     /note="metagenomic"
     gene            complement(376..933)
                     /locus_tag="PLF56_10960"
     CDS             complement(376..933)
                     /locus_tag="PLF56_10960"
                     /inference="COORDINATES: protein motif:HMM:NF020989.2"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="DUF2017 domain-containing protein"
                     /protein_id="HOQ54118.1"
                     /translation="MTRFKKSGKQILITLADYEEAVLESMIEQLIGLIEGDGVHAAAD
                     DPFARWEADMAEPGGMDASDPVIARLFPPAYRDDPGAEHEFRRFTEVAQRRTKIDQAQ
                     VVLNALLETNAGRDPAVVRPVDVEAWLKTITAIRLSLSVRLGIETADDIDELEQLDDD
                     EPRAVLYSMYEWLSYFLDSLLDALH"
     gene            complement(930..1244)
                     /gene="clpS"
                     /locus_tag="PLF56_10965"
     CDS             complement(930..1244)
                     /gene="clpS"
                     /locus_tag="PLF56_10965"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_013160672.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_process: GO:0006508 - proteolysis [Evidence IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="ATP-dependent Clp protease adapter ClpS"
                     /protein_id="HOQ54119.1"
                     /translation="MAFASVEPAVPEGGTVLAERPQSTPVELVTWVTIVWDDPVNLMS
                     YVTHVFVTYFGYSKSKATKLMLQVHNEGRSVVSSGTREAMETDVSAMHGYGLWASLER
                     AQ"
     gene            1297..2643
                     /locus_tag="PLF56_10970"
     CDS             1297..2643
                     /locus_tag="PLF56_10970"
                     /EC_number="6.3.4.21"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_013864751.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_function: GO:0004514 - nicotinate-nucleotide
                     diphosphorylase (carboxylating) activity [Evidence IEA];
                     GO_function: GO:0004516 - nicotinate
                     phosphoribosyltransferase activity [Evidence IEA];
                     GO_process: GO:0009435 - NAD biosynthetic process
                     [Evidence IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="nicotinate phosphoribosyltransferase"
                     /protein_id="HOQ54120.1"
                     /translation="MQNSTALLTDHYELTMVRAALRSGAAFRPSVFELFPRRLPTGRR
                     YGVVAGVGRALEAIEAFTFDQATIDWLLQHDVVDEPLANWLAGYRFSGSIWGYPEGEV
                     YFPGSPLMIVEGSFAEAVILETVLLSVYNYDSAVASAASRMTAMARDRPCIEMGSRRT
                     NEHSAVAAARAAYVAGFAATSNLQAGLRYGVPTRGTAAHSFTLLHATESQAFEAQIGA
                     LGEDTTLLVDTYDIETAVRTGVELTKGKLGAIRLDSGDLAELAGDVRQLLDSLGATST
                     RILVTSDLDEWQIAALRGAPVDGFGVGTSLVTGSGAPTCGFVYKLVARADSDEPGAPL
                     VPVAKKSVNKTSIGGRKFALRRLDARGKAEAEVIGIGAPPNGDTNDRPLLVELVRDGE
                     IVGAEALEVGQRRHARSRAELPLSALKMSKGEAAIPTLMLDEHGVAAENPYARSEA"
     gene            2640..3191
                     /locus_tag="PLF56_10975"
     CDS             2640..3191
                     /locus_tag="PLF56_10975"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_020387119.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="isochorismatase family protein"
                     /protein_id="HOQ54121.1"
                     /translation="MSRVLVVVDVQNDFCEGGSLAVAGGAATASAISAVVAAGAYGHV
                     VATRDHHIDPGGHFSATPDFVDSWPPHCVAGTPGADFHAGLIASPSFEAVFDKGEYAA
                     AYSGFEGLDGGVSLAAWLRQHGASEVEVCGIATDYCVKATALDAARLGYPTTVLLGLT
                     AAVNPGDVPAVLAELQAAGVRVG"
BASE COUNT          519 a         1111 c         1124 g          555 t
ORIGIN      
        1 gagggcagtc atgtcgggtc gccaatcctc ggtcgagaaa gctggaacga agcaagcctg
       61 tcctgaccac agtgctacgt ggcagtcgcg actggcgcca tccggccggc tcgggcctcg
      121 acatcgtccg ggtcagctat gcctagccta agcagcagca gcactactcg tcggcgatcc
      181 accgaatttc ccggacgccg gtcatccgtc gtccacattc tgaacatcgt ctcagatgcg
      241 gatgacacga ccccactggg tcacttcagt gcagcgcgtc cagcagagtc agttcagtgc
      301 agcgcgtcca gcagagtcag ttcagtgcag cgcgccagca gagtcagttc agtgcagcgc
      361 gccagcagag tcagttcagt gcagcgcgtc cagcaggctg tcgagaaagt agctgagcca
      421 ctcgtacatg ctgtagagca cggcgcgcgg ctcgtcgtcg tcgagttgct cgagttcgtc
      481 gatgtcgtcc gccgtctcga tacccaaccg caccgacagg ctcaaccgaa tcgctgtgat
      541 ggtcttcagc caggcctcga cgtcgaccgg gcggacgacc gcggggtcgc ggccggcgtt
      601 ggtttcgagc agcgcgttga gaaccacctg ggcctggtcg atcttcgtgc ggcgctgcgc
      661 gacctcggtg aaccgccgga actcgtgctc tgcgcccgga tcgtcgcggt aggcgggcgg
      721 aaacagacga gcgatcaccg ggtcggaggc gtccatgccc cctggttcgg ccatgtcggc
      781 ctcccaccgg gcgaaggggt cgtcggccgc cgcgtgcacg ccgtcgccct cgatcaggcc
      841 gatcagctgc tcgatcatcg attcgagtac ggcctcttca tagtcggcca gggtgatcag
      901 aatctgcttg cccgacttct tgaaccgcgt cattgcgccc gctccaggct ggcccacagg
      961 ccgtagccgt gcatggcgct gacgtcggtc tccatggcct cgcgggtgcc cgacgacacc
     1021 accgacctgc cctcgttgtg cacctgcagc atcagtttgg tggccttcga cttggagtag
     1081 ccgaagtacg tcacgaacac gtgggtgacg tagctcatca ggttgaccgg gtcgtcccag
     1141 acgatcgtca cccacgtgac cagctcgacc ggcgtgctct gcggacgctc cgcgagcacc
     1201 gtgccaccct cgggcacggc gggttcaacg gacgcaaaag ccatgctggg cattgtgtca
     1261 gaacggggtg gatgcgtacc cgttacgctg gcgcgcatgc agaactcgac ggcgctgttg
     1321 accgaccact atgagctgac catggtgcgg gcggcgctgc gctcgggtgc cgcgttccgg
     1381 ccctcggtct tcgaattgtt tccccgtcga ttgcccaccg gacgccgcta cggcgtggtc
     1441 gccggcgtcg gccgagccct cgaagcgatc gaggccttca ccttcgacca ggccaccatc
     1501 gactggctgt tgcagcacga cgtcgtggac gagccgctgg cgaactggct ggccggctac
     1561 cggttcagcg gcagcatctg gggctacccc gagggcgagg tctactttcc tggatcgccg
     1621 ctgatgatcg tcgagggatc cttcgccgag gcggtgattc tcgaaacggt gctgctcagc
     1681 gtctacaact acgactcggc ggtcgcctcg gccgcttcgc gcatgacggc catggcgcgc
     1741 gatcgtccgt gcatcgaaat gggctcgcga cgcaccaacg aacactccgc cgtggcggcc
     1801 gcacgagcgg cttacgtggc cggtttcgcg gcgacgtcca atctgcaggc gggcctgcgc
     1861 tacggagtgc cgacccgggg cacagcggca cacagcttca ccctgctgca cgccaccgag
     1921 tcgcaggcgt tcgaggcgca gatcggggcg ctgggcgaag acaccacgct gctggtcgac
     1981 acctacgaca tcgagaccgc cgtgcgcacc ggcgtcgaac tgaccaaggg caaactgggc
     2041 gcgatccggc tcgattcggg cgacctggcc gagttggccg gtgacgtgcg gcagttgctc
     2101 gactcgttgg gcgccaccag tacccgcatt ctggtcacct ccgatctgga cgagtggcag
     2161 atcgccgcgc tgcgcggcgc ccccgtcgac ggcttcggtg tgggcacctc gctggtcacc
     2221 ggctcgggcg ccccgacctg cggcttcgtg tacaaactgg tggcccgggc cgacagcgac
     2281 gagcctggcg caccgctcgt cccggtggcg aagaagagcg tgaacaagac cagcatcggc
     2341 ggacgcaagt tcgcgctccg gcggctggac gcccgaggca aggccgaggc cgaggtgatc
     2401 ggcatcgggg cgccgccgaa cggcgacacg aacgatcgtc cgctgttggt ggagttggtg
     2461 cgcgacggcg agatcgtcgg cgccgaggcg ctcgaggtcg ggcagcggcg gcacgcccgt
     2521 tcacgcgccg aactaccgct gtcggcgctc aagatgagca agggtgaggc ggccatccca
     2581 accctgatgc tcgatgagca cggcgtggcc gccgagaacc cgtacgcccg gagcgaggca
     2641 tgagccgggt tctggtcgtc gtggacgtgc agaacgactt ctgcgagggc gggtcgctgg
     2701 cggtggccgg cggtgctgcg acggcgtccg cgatctcggc tgtggtggcc gccggcgcgt
     2761 atggccatgt ggtggcgacc cgcgaccatc acatcgatcc cggcggccac ttctcggcca
     2821 cccccgactt cgtggattcg tggccgccgc actgtgtcgc aggcacaccg ggcgccgact
     2881 ttcacgccgg cctgatcgcc tcccccagct tcgaagcggt gttcgacaag ggtgaatacg
     2941 cggcggccta ctcgggtttc gagggcctgg acggcggggt ctcgctcgcg gcgtggttgc
     3001 ggcagcacgg agcgtccgag gtcgaggtgt gcggcattgc cacggactac tgcgtcaagg
     3061 ccaccgccct ggacgccgcc cgcctcgggt atccgaccac cgtgctgctc ggcctgaccg
     3121 cagcggtcaa cccgggcgac gtgccggccg tgctggccga actgcaggcg gcgggcgtgc
     3181 gggtcggctg agcgggagga cgcttctcag cacggtttca gggatggttc tcacaccact
     3241 cagccgacgg cacgccgcac cagctgagcg aactgcttct tcgcctcgtc cgtcatcgtg
     3301 gtgatcgca
//
LOCUS       DAOSUG010000357         3308 bp    DNA     linear   ENV 10-JUN-2024
DEFINITION  MAG TPA_asm: Micropruina sp. isolate AMR_MDS_2195
            NODE_11813_length_3308_cov_3.509683, whole genome shotgun sequence.
ACCESSION   DAOSUG010000357 DAOSUG010000000
VERSION     DAOSUG010000357.1
DBLINK      BioProject: PRJNA925477
            BioSample: SAMN32864262
            Sequence Read Archive: SRR4236673
KEYWORDS    WGS; ENV; Metagenome Assembled Genome; MAG; Third Party Data; TPA;
            TPA:assembly.
SOURCE      Micropruina sp. (wastewater metagenome)
  ORGANISM  Micropruina sp.
            Bacteria; Bacillati; Actinomycetota; Actinomycetes;
            Propionibacteriales; Nocardioidaceae; Micropruina.
REFERENCE   1  (bases 1 to 3308)
  AUTHORS   Abdulkadir,N., Saraiva,J.P., Zhang,J., Stolte,S., Gillor,O.,
            Harms,H. and Rocha,U.
  TITLE     Genome-centric analyses of 165 metagenomes show that mobile genetic
            elements are crucial for the transmission of antimicrobial
            resistance genes to pathogens in activated sludge and wastewater
  JOURNAL   Microbiol Spectr 12 (3), e0291823 (2024)
   PUBMED   38289113
REFERENCE   2  (bases 1 to 3308)
  AUTHORS   Abdulkadir,N.
  TITLE     Direct Submission
  JOURNAL   Submitted (24-MAR-2023) Environmental Microbiology, Helmholtz
            Center for Environmental Research -UFZ, Permoserstr 15, Leipzig
            04318, Germany
COMMENT     The annotation was added by the NCBI Prokaryotic Genome Annotation
            Pipeline (PGAP). Information about PGAP can be found here:
            https://www.ncbi.nlm.nih.gov/genome/annotation_prok/
            
            ##Genome-Assembly-Data-START##
            Assembly Method        :: SPAdes v. 3.11.1
            Genome Representation  :: Full
            Expected Final Version :: Yes
            Genome Coverage        :: Not Applicable
            Sequencing Technology  :: Illumina MiSeq
            ##Genome-Assembly-Data-END##
            
            ##Genome-Annotation-Data-START##
            Annotation Provider               :: NCBI
            Annotation Date                   :: 05/04/2023 04:57:10
            Annotation Pipeline               :: NCBI Prokaryotic Genome
                                                 Annotation Pipeline (PGAP)
            Annotation Method                 :: Best-placed reference protein
                                                 set; GeneMarkS-2+
            Annotation Software revision      :: 6.5
            Features Annotated                :: Gene; CDS; rRNA; tRNA; ncRNA
            Genes (total)                     :: 2,776
            CDSs (total)                      :: 2,750
            Genes (coding)                    :: 2,714
            CDSs (with protein)               :: 2,714
            Genes (RNA)                       :: 26
            tRNAs                             :: 24
            ncRNAs                            :: 2
            Pseudo Genes (total)              :: 36
            CDSs (without protein)            :: 36
            Pseudo Genes (ambiguous residues) :: 0 of 36
            Pseudo Genes (frameshifted)       :: 2 of 36
            Pseudo Genes (incomplete)         :: 32 of 36
            Pseudo Genes (internal stop)      :: 2 of 36
            ##Genome-Annotation-Data-END##
FEATURES             Location/Qualifiers
     source          1..3308
                     /organism="Micropruina sp."
                     /mol_type="genomic DNA"
                     /submitter_seqid="NODE_11813_length_3308_cov_3.509683"
                     /isolate="AMR_MDS_2195"
                     /isolation_source="Activated sludge of municipal
                     wastewater treatment plants"
                     /db_xref="taxon:2737536"
                     /environmental_sample
                     /geo_loc_name="Canada"
                     /lat_lon="49.25 N 123.1 W"
                     /collection_date="2015-06-16"
                     /metagenome_source="wastewater metagenome"
                     /note="metagenomic"
     gene            complement(<1..138)
                     /locus_tag="PLF56_10980"
     CDS             complement(<1..138)
                     /locus_tag="PLF56_10980"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_020388514.1"
                     /note="catalyzes the oxygen-independent formation of
                     protoporphyrinogen-IX from coproporphyrinogen-III; Derived
                     by automated computational analysis using gene prediction
                     method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="coproporphyrinogen III oxidase"
                     /protein_id="HOQ54122.1"
                     /translation="MPSALPSGEPAPSDGSLPAPALRELGDGPLSFYLHVPFCATRCG
                     YC"
     gene            complement(138..944)
                     /locus_tag="PLF56_10985"
     CDS             complement(138..944)
                     /locus_tag="PLF56_10985"
                     /EC_number="2.4.2.28"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_007078965.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_function: GO:0017061 - S-methyl-5-thioadenosine
                     phosphorylase activity [Evidence IEA];
                     GO_process: GO:0009116 - nucleoside metabolic process
                     [Evidence IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="S-methyl-5'-thioadenosine phosphorylase"
                     /protein_id="HOQ54123.1"
                     /translation="MQQADIGIIGGSGFYSFLSEPERIEIDTPFGEPSAPLAVGDVNG
                     RRVAFVPRHGEQHQFPPHRVNYRANLWALRSLGVRQVLAPCAVGGLRADLAPGTFVVP
                     DQVVDRTWGRAHTIWGAIGPVVHTSFADPYCPRGRSALLRANPAAVDGGTLVVINGPR
                     FSSRAESQWHAAAGWLVVGMTGMPEASVARDLAMCFSTVCMVTDSDAGVEGPGVSHAE
                     VLRVFAGNIERLKAMLLDVIGALPEPESDETATCACRRVLDGLPLPFDLP"
     gene            complement(1134..2501)
                     /locus_tag="PLF56_10990"
     CDS             complement(1134..2501)
                     /locus_tag="PLF56_10990"
                     /inference="COORDINATES: protein motif:HMM:NF024808.2"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_component: GO:0055052 - ATP-binding cassette (ABC)
                     transporter complex, substrate-binding subunit-containing
                     [Evidence IEA];
                     GO_function: GO:0042626 - ATPase-coupled transmembrane
                     transporter activity [Evidence IEA];
                     GO_function: GO:0140359 - ABC-type transporter activity
                     [Evidence IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="sugar ABC transporter substrate-binding protein"
                     /protein_id="HOQ54124.1"
                     /translation="MSTASTLSRRTLMTGLAGAALIGAAGCSPAAQQTASAPTATGSG
                     LTGTVTVRLWDEQVQKAYDASFAEFKKANPGITVKTVLVPWADYFTKLRTDVGGGTAD
                     DIFMMNGSYLQPYVTSNSLLEIGSAFDSLKADWVPAAVEQYTRDGKLWGVPQLTDGGI
                     AVYYNKKLLDDAGITPDQLNGLVWAPGGGSTDTLLPILKKLTIDAAGKRGDESGFNGA
                     KPKQWGYSAAQDLQGIYYNFIGSNGGQFQDPDGRFVFASPESVAAFQYIVDLINKYKV
                     SPAASNTNDNGDFTRDQFLQGKIALFQSGVYNLKNVSDGAKFEWGIAPLPAGPKGAVS
                     VVNSVIVSGNAKSKSPELTQKVLQWLGSGDGAKFIGSTGAAMPAVTSAQSAFNDFWKG
                     KNVDPSQFAKQGALPSISAPAGENYGAAFAAWKPIFNEIFLGRTPVAEGLQKAQDAAN
                     KAAGS"
     gene            complement(2562..>3308)
                     /locus_tag="PLF56_10995"
     CDS             complement(2562..>3308)
                     /locus_tag="PLF56_10995"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_020579018.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_process: GO:0008643 - carbohydrate transport [Evidence
                     IEA];
                     GO_process: GO:0055085 - transmembrane transport [Evidence
                     IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="carbohydrate ABC transporter permease"
                     /protein_id="HOQ54125.1"
                     /translation="LSFMTAFKSPQQFASESSLALPNPWTTENFARLFGGDYGFVSPI
                     VVTAQVVAVILVGQLLFSVMAAYAFAKLQFRGRDALFWVYLATLMVPQVVIVIPLFVM
                     MSQLGLRNTFWSLVLPFMFGSPYAVFLLREHFRSIPDDLIDAARIDGAGDWRILWQIV
                     APLSRGIVSTLAIITVVSHWNNFMWPLIITSGRDWQVITVATANLQSQYNANWTLVMA
                     ATTVAMAPLLVLFAIFQRTIVESISLTGFK"
BASE COUNT          569 a         1092 c         1131 g          516 t
ORIGIN      
        1 gcagtagccg caccgggtcg cacagaaggg cacgtgcagg tagaacgaga gcggtccgtc
       61 ccccagttcg cgcaacgccg gtgcgggcaa cgacccgtcg gacggcgcgg gctcacccga
      121 cggcagcgcc gacggcatca gggcaggtcg aacggcaacg gcagaccgtc cagcacccgc
      181 cggcaggcgc aggtggccgt ctcgtccgat tcgggctcgg gtagggcgcc gatcacgtcc
      241 agcagcatgg ccttgagccg ctcgatgttg ccggcgaaca cccgcagcac ctcggcgtgc
      301 gacacccccg gaccttcgac gccggcgtcc gaatcggtca ccatgcagac cgtcgagaag
      361 cacatcgcca ggtcgcgcgc gacggacgcc tccggcatgc cggtcatgcc gaccaccaac
      421 cagccggcgg cggcgtgcca ttgcgactcg gcccggctcg agaaccgcgg gccgttgatc
      481 acgaccaggg tgccgccgtc caccgccgcc ggattggccc gtagcagcgc ggaccggccg
      541 cgcgggcagt acgggtcggc gaacgaggtg tgcacgacgg ggccgatcgc gccccagatg
      601 gtgtgcgccc gtccccaggt gcggtcgacg acctggtcgg gtacgacgaa ggtgcccggg
      661 gcgaggtcgg cccgcagacc accgacggcg cagggggcca gcacctgccg caccccgagc
      721 gaacgcaggg cccacaggtt ggcccggtag ttcacccggt gcggcgggaa ctggtgctgc
      781 tcgccgtggc ggggcacgaa ggcgacccgg cgtccgttga cgtcaccgac ggccagcggc
      841 gcgctgggtt cgccgaacgg ggtgtcgatc tcgatgcgtt cgggctcgct cagaaacgag
      901 tagaaccccg agccgccgat gatgccgatg tctgcctgct gcacggcccc actttaggac
      961 gaacggctcg gggacggccg cccctgacga cgttgcgcgg cttggcgttc attgcgcgcg
     1021 gacgaacccg cgcaaccagc cctggaccgc gcgatgacgt gtcggctcct cgaccagcgc
     1081 ggtgccgaca cgctcgacca gcggtatggt cccgacgaac ctcgaccagc ggactacgaa
     1141 ccggccgcct tgttggctgc gtcctgggcc ttctgcaggc cttcggccac gggcgtgcga
     1201 cccaggaaga tctcgttgaa gatcggcttc caggccgcga acgccgcgcc gtagttctca
     1261 cccgcgggcg ccgagatcga cggcagtgca ccctgcttgg cgaactggga cggatcgacg
     1321 ttcttgccct tccagaagtc gttgaaggcg gactgcgcgc tggtcaccgc gggcatcgcc
     1381 gcgccggtcg agccgatgaa cttggcgccg tcgccggagc ccagccactg cagcaccttc
     1441 tgggtcaact cggggctctt gctcttggcg ttgcctgaca cgatcaccga gttgaccacc
     1501 gacacggcac ccttggggcc ggccggcagg ggcgcgatgc cccactcgaa cttggcgccg
     1561 tccgacacgt tcttcaggtt gtacacaccc gactggaaca gtgcgatctt gccctgcaag
     1621 aactgatcgc gggtgaagtc accgttgtcg ttggtgttgg acgcggccgg cgacaccttg
     1681 tacttgttga tcaggtccac gatgtactgg aacgccgcca ccgactcggg cgaggcgaag
     1741 acgaacctgc cgtcggggtc ctggaactgg ccaccgttgc tgccgatgaa gttgtagtag
     1801 atgccctgca ggtcctgggc cgcggagtag ccccactgct tgggcttggc gccgttgaag
     1861 ccgctctcgt cgccccgctt gccggcggca tcgatcgtga gcttcttcag gatcggcagc
     1921 agcgtgtcgg tactgccgcc gccgggcgcc cagaccaggc cgttgagctg atcgggggtg
     1981 atgccggcgt cgtccagcaa cttcttgttg tagtacacgg cgatgccgcc gtcggtgagc
     2041 tgcgggacgc cccacaactt gccgtcgcgg gtgtattgct cgacggccgc gggcacccag
     2101 tcggccttca gcgagtcgaa cgcggagccg atttcgagca gcgagttgga cgtgacgtac
     2161 ggctgcaggt agctgccgtt catcatgaag atgtcgtcgg cggtaccgcc acccacgtcg
     2221 gtgcgcagct tggtgaagta gtcggcccac ggtaccagca cggtcttgac cgtgatgccc
     2281 gggttggcct tcttgaactc ggcgaacgag gcgtcgtagg ccttctgcac ctgctcgtcc
     2341 cacagtcgga cggtcacggt gccggtcaag ccggacccgg tcgcggtggg cgccgaagcg
     2401 gtctgctgtg cggcggggga acagccggcg gcaccgatca gggccgcgcc ggccaggccg
     2461 gtcatgagag tgcgtcggct cagggtggat gccgtagaca cagggcttct ccttctagaa
     2521 gttggtagct caataatcac atcattggac ccgaccgagg ttcatttgaa accggtcagt
     2581 gagatcgatt cgacgatcgt tcgctggaag atcgcgaaca gcaccagcag tggtgccatc
     2641 gcgaccgtgg tggcggccat caccagagtc cagttggcgt tgtactggct ctgcaggttg
     2701 gccgtggcga ccgtgatcac ctgccaatcg cgtccgctgg tgatgatcaa cggccacatg
     2761 aagttgttcc agtggctgac cacggtgatg atcgcgagcg tcgagacgat gccccgcgac
     2821 agcggcgcca cgatctgcca gagaatgcgc cagtcgcccg cgccgtcgat acgggcggcg
     2881 tcgatcaggt cgtcgggaat gctgcgaaag tgctcgcgca gcaggaacac cgcatagggc
     2941 gagccgaaca tgaacggcag caccagcgac cagaacgtgt tgcgcagccc gagttgcgac
     3001 atcatcacga acagcggaat gacgatcacc acctggggca ccatcagcgt cgccaggtac
     3061 acccagaaca gcgcgtcacg gccgcggaac tgcagcttgg cgaacgcgta ggcggccatc
     3121 accgagaaca acagctggcc gaccagaatc accgccacca cctgggcggt gaccacgatg
     3181 gggctgacga agccgtagtc gcccccgaac agccgggcga agttctcggt cgtccagggg
     3241 ttcggcaacg cgagggacga ttcggacgcg aactgctgcg gcgacttgaa cgcggtcatg
     3301 aagctgag
//
LOCUS       DAOSUG010000358         3295 bp    DNA     linear   ENV 10-JUN-2024
DEFINITION  MAG TPA_asm: Micropruina sp. isolate AMR_MDS_2195
            NODE_11875_length_3295_cov_4.623765, whole genome shotgun sequence.
ACCESSION   DAOSUG010000358 DAOSUG010000000
VERSION     DAOSUG010000358.1
DBLINK      BioProject: PRJNA925477
            BioSample: SAMN32864262
            Sequence Read Archive: SRR4236673
KEYWORDS    WGS; ENV; Metagenome Assembled Genome; MAG; Third Party Data; TPA;
            TPA:assembly.
SOURCE      Micropruina sp. (wastewater metagenome)
  ORGANISM  Micropruina sp.
            Bacteria; Bacillati; Actinomycetota; Actinomycetes;
            Propionibacteriales; Nocardioidaceae; Micropruina.
REFERENCE   1  (bases 1 to 3295)
  AUTHORS   Abdulkadir,N., Saraiva,J.P., Zhang,J., Stolte,S., Gillor,O.,
            Harms,H. and Rocha,U.
  TITLE     Genome-centric analyses of 165 metagenomes show that mobile genetic
            elements are crucial for the transmission of antimicrobial
            resistance genes to pathogens in activated sludge and wastewater
  JOURNAL   Microbiol Spectr 12 (3), e0291823 (2024)
   PUBMED   38289113
REFERENCE   2  (bases 1 to 3295)
  AUTHORS   Abdulkadir,N.
  TITLE     Direct Submission
  JOURNAL   Submitted (24-MAR-2023) Environmental Microbiology, Helmholtz
            Center for Environmental Research -UFZ, Permoserstr 15, Leipzig
            04318, Germany
COMMENT     The annotation was added by the NCBI Prokaryotic Genome Annotation
            Pipeline (PGAP). Information about PGAP can be found here:
            https://www.ncbi.nlm.nih.gov/genome/annotation_prok/
            
            ##Genome-Assembly-Data-START##
            Assembly Method        :: SPAdes v. 3.11.1
            Genome Representation  :: Full
            Expected Final Version :: Yes
            Genome Coverage        :: Not Applicable
            Sequencing Technology  :: Illumina MiSeq
            ##Genome-Assembly-Data-END##
            
            ##Genome-Annotation-Data-START##
            Annotation Provider               :: NCBI
            Annotation Date                   :: 05/04/2023 04:57:10
            Annotation Pipeline               :: NCBI Prokaryotic Genome
                                                 Annotation Pipeline (PGAP)
            Annotation Method                 :: Best-placed reference protein
                                                 set; GeneMarkS-2+
            Annotation Software revision      :: 6.5
            Features Annotated                :: Gene; CDS; rRNA; tRNA; ncRNA
            Genes (total)                     :: 2,776
            CDSs (total)                      :: 2,750
            Genes (coding)                    :: 2,714
            CDSs (with protein)               :: 2,714
            Genes (RNA)                       :: 26
            tRNAs                             :: 24
            ncRNAs                            :: 2
            Pseudo Genes (total)              :: 36
            CDSs (without protein)            :: 36
            Pseudo Genes (ambiguous residues) :: 0 of 36
            Pseudo Genes (frameshifted)       :: 2 of 36
            Pseudo Genes (incomplete)         :: 32 of 36
            Pseudo Genes (internal stop)      :: 2 of 36
            ##Genome-Annotation-Data-END##
FEATURES             Location/Qualifiers
     source          1..3295
                     /organism="Micropruina sp."
                     /mol_type="genomic DNA"
                     /submitter_seqid="NODE_11875_length_3295_cov_4.623765"
                     /isolate="AMR_MDS_2195"
                     /isolation_source="Activated sludge of municipal
                     wastewater treatment plants"
                     /db_xref="taxon:2737536"
                     /environmental_sample
                     /geo_loc_name="Canada"
                     /lat_lon="49.25 N 123.1 W"
                     /collection_date="2015-06-16"
                     /metagenome_source="wastewater metagenome"
                     /note="metagenomic"
     gene            198..899
                     /gene="aat"
                     /locus_tag="PLF56_11000"
     CDS             198..899
                     /gene="aat"
                     /locus_tag="PLF56_11000"
                     /EC_number="2.3.2.6"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_013865983.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_function: GO:0008914 - leucyltransferase activity
                     [Evidence IEA];
                     GO_process: GO:0006508 - proteolysis [Evidence IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="leucyl/phenylalanyl-tRNA--protein transferase"
                     /protein_id="HOQ54126.1"
                     /translation="MSRPGIFGPARDWPAQDLIGLSDDVDPDLVVLAYRCGVFPMPLH
                     NSWFPARMGWWSPMRRGVLLPDGLRVTRSLRKAAHHYTTTVDAAFDRVLAGCADPSRP
                     HGWIDDDIVAVFTDLHHRGVVHSVETWTPDGRLAGGLYGVSLGGLFAGESMFHDPEIG
                     RDASKVALLRLVAAMTRDGSADRIIDVQWRTDHLGSLGVIEIDRDEYLSALDETLVLP
                     EIDWTAERRREPEVA"
     gene            892..1755
                     /locus_tag="PLF56_11005"
     CDS             892..1755
                     /locus_tag="PLF56_11005"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_013865967.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_function: GO:0003906 - DNA-(apurinic or apyrimidinic
                     site) endonuclease activity [Evidence IEA];
                     GO_function: GO:0008270 - zinc ion binding [Evidence IEA];
                     GO_function: GO:0019104 - DNA N-glycosylase activity
                     [Evidence IEA];
                     GO_process: GO:0006284 - base-excision repair [Evidence
                     IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="DNA-formamidopyrimidine glycosylase family
                     protein"
                     /protein_id="HOQ54127.1"
                     /translation="MPELPEVEALRSFLHDRLIGQTIARTELLAFSALKTFQLPLAAL
                     HGAEITDVRRYGKFLAIDAGGTWLVFHLARAGWLTWKDAMPAKPARPGRGPLSLRVVL
                     ADDSGFDLTEMGTQRKLAVYVVGDPSEIAMVSTLGPDPLADDFDSAALAAILARAGRA
                     QLKGVLRDQRVLAGIGNAYSDEILHAARLSPYKPSNALSADEHERLFGAIKNELGDAI
                     ARSVGRAAKELKGEKKIGLRVHGRTGEPCDVCGTAIAEVSFADSSLQYCPGCQTGGKL
                     LADRRLSKLLK"
     gene            complement(2109..2393)
                     /locus_tag="PLF56_11010"
     CDS             complement(2109..2393)
                     /locus_tag="PLF56_11010"
                     /inference="COORDINATES: ab initio
                     prediction:GeneMarkS-2+"
                     /note="Derived by automated computational analysis using
                     gene prediction method: GeneMarkS-2+."
                     /codon_start=1
                     /transl_table=11
                     /product="hypothetical protein"
                     /protein_id="HOQ54128.1"
                     /translation="MIRAALALSIALFGAVSATPSANAAVDSSVQQVSQRISYWQYNY
                     YHNYFTSSTTCNNRGYAMLNYDQIYGMIDYRCHKNAGESKWSMDVLWRAG"
     gene            2766..>3295
                     /locus_tag="PLF56_11015"
     CDS             2766..>3295
                     /locus_tag="PLF56_11015"
                     /inference="COORDINATES: ab initio
                     prediction:GeneMarkS-2+"
                     /note="Derived by automated computational analysis using
                     gene prediction method: GeneMarkS-2+."
                     /codon_start=1
                     /transl_table=11
                     /product="hypothetical protein"
                     /protein_id="HOQ54129.1"
                     /translation="MPSALIVKARAVLASPWTSNSRLGLRLIVGATLLTVAIAFLGPS
                     AVALRLGPRTTLMPPWYLPAGWVQLNEWVAVVSLWVAIAAGAIGMWICYRAVEAGWRP
                     HVRKLFLLGAALSTVTALVPPLTSADVLMYAAYGRLMVLGWNPYDITPANIIRQEYDP
                     VMRWVEAPWQDTPSVYG"
BASE COUNT          581 a         1056 c         1077 g          581 t
ORIGIN      
        1 cactttgtgg ttgaaatgat cgcgggtcgt ccgcgtgatc gaacccagcg ccactttctg
       61 gttgaaataa atacccatag ggggtatcta tttcaaccac aaagtggcgc ggcgcagcgg
      121 agctgcttct tgcacccgct ataaccacca gaaagtggcg aaaccctata cgcaagccgt
      181 ggtggcacaa tcgggccatg agtcgacccg gcatcttcgg ccctgcgcgg gactggcccg
      241 cgcaagacct gatcgggctg tcggacgacg tcgaccccga tctggtggtg ctggcctacc
      301 gctgcggcgt gttccctatg ccactgcaca actcgtggtt tccggcccgc atgggttggt
      361 ggtcgccgat gcggcgcggc gtgctgctgc ccgacgggct gcgggttacc cggtcactgc
      421 gcaaggccgc gcaccactac accaccaccg tcgacgccgc gttcgaccgg gtgctggccg
      481 gctgcgccga cccgtcgagg ccgcacgggt ggatcgacga cgacatcgtg gccgtgttca
      541 ccgacctgca ccaccggggg gtggtgcaca gcgtcgagac ctggacgccc gacggacgcc
      601 tggccggcgg cctctacggc gtgtcgctgg gtgggctgtt cgcgggcgag tccatgtttc
      661 acgaccccga gatcggacgg gacgcgtcca aggtcgcgct gctgcggctg gtggcggcga
      721 tgacccgcga cggatccgcg gaccggatca tcgacgtgca gtggcgcacc gaccacctgg
      781 gcagcctcgg cgtgatcgag atcgaccgcg acgagtatct gtcggcgctg gacgagacgc
      841 tggtgctgcc cgagatcgac tggacggctg agcgacgaag ggagcccgag gttgcctgaa
      901 ctgcccgagg tcgaggcgct gcggtcgttt ctgcatgacc ggctgatcgg ccagaccatc
      961 gcccgcaccg agctactcgc cttcagcgcc ctgaagacct ttcaactgcc gctggccgcg
     1021 ctgcacgggg ccgagatcac cgacgtgcgt cgctacggca agtttctggc catcgacgcc
     1081 ggcggcacct ggctggtgtt tcacctggcc agggccggtt ggctgacctg gaaggacgcc
     1141 atgccggcca agcctgcccg gcccggtcgc ggcccgctga gcctgcgcgt ggtgttggcc
     1201 gacgactccg gtttcgacct cacagagatg ggcacccaac gcaagctggc ggtgtacgtg
     1261 gtgggcgacc cgtccgagat cgcgatggtg agcacgctgg gccccgaccc gctcgcggac
     1321 gatttcgact ccgcggcgct ggccgcgatt ctggccaggg ccggacgggc ccagctcaag
     1381 ggtgtgctgc gcgaccagcg ggtgctggcc ggtatcggca acgcctacag cgacgagatt
     1441 ctgcacgccg ccaggctcag cccgtacaag ccgtccaacg ccctcagcgc cgacgagcac
     1501 gagcggctgt tcggggcgat caagaacgaa ctcggcgacg cgatcgcacg ctcggtcggc
     1561 cgggccgcca aagaactgaa gggcgagaag aagatcggcc tgcgggtgca cggacgcacc
     1621 ggcgaaccct gtgacgtgtg cggcaccgcg atcgcggagg tgtccttcgc ggactcgtcc
     1681 ctgcagtact gcccaggctg ccagaccggc ggcaagctgc tggccgatcg acgactgtcc
     1741 aaactgctca agtagctggg tcgaccgata ctggccagac atggatttcc ccagatccca
     1801 catcagttag ccaatccgtt gtaatcgcaa cgccaagagg atgagcgaca gccgcggcag
     1861 ctacaacggc attgtcgaac gtctctttgg ggatgcgccg gaagtagatt gtgaggggtg
     1921 gtgcgccctc tggcgatatc agagcgaacg cgctatgcca tatggcagtc tcgaaactca
     1981 acacacggtc gtctgccgca ttcgagatgc tggcctgcac tctcttcgcc aggcacgtac
     2041 cggaactcgg ccaaggattc agagcagcca agagacgctt caactcgtca agacgcagcg
     2101 tcgtcacgtc acccagcccg ccacagaaca tccatactcc acttactttc ccccgcattc
     2161 ttgtggcacc tgtaatcgat catgccgtag atttgatcgt aattgagcat ggcgtagccg
     2221 cggttgttgc aggtggttga actggtgaaa tagttatgat aatagttata ttgccagtat
     2281 gagatgcgtt gtgagacttg ttgtacactt gaatcaacag cggcatttgc tgacggggtt
     2341 gctgacaccg caccaaagag ggcaatgctg agtgcgagtg cggctcgaat caaaactata
     2401 agcatactac cccatgacca aaatgcgacg gcccaaatga tggtgtgaag ctacgcccct
     2461 cgtccgagta tgtcaacaac ttcccacgtc tcgtctttaa tcccttcgga atgaagccaa
     2521 tagcctgtgt acatcgcgca tggggtcaga cctctacgac cgagcgcaca tcaggagcga
     2581 ggcaatcgcg aaacggcttg ttcagcgccg gtcggtagca gttcggcgga cgagatgacc
     2641 gcgctcgtcc gcgatgatgg tgcactcgcg ataacaagct ggtctcgacc tgggcgcgcg
     2701 ggtggggtgc ggacggcgcc gccgctagtc tcggccttgc cgtccgaacc ccgaggagca
     2761 acccgatgcc gagcgcgttg atcgtcaagg cgcgcgccgt cctcgcctca ccctggacga
     2821 gcaactccag gctcggcttg cggctgatcg tcggcgccac cctgctcacc gtggcgatcg
     2881 cctttctggg gccgtccgcc gtggccctgc ggctcggccc gcgcaccaca ctgatgccgc
     2941 cgtggtatct gccggccggc tgggtgcagc tcaacgagtg ggtggcggtg gtctcgctgt
     3001 gggtggcgat cgcggcgggg gcgatcggca tgtggatctg ttaccgggcc gtcgaggcgg
     3061 gctggcgccc gcacgtccgc aagctgttcc tactgggggc cgcactgtcg accgtgaccg
     3121 ccctggtgcc gccgctgacg tcggcggacg tgctgatgta cgccgcctac ggacggctga
     3181 tggtgctggg ctggaacccc tacgacatca cgcccgccaa catcatccgg caggagtacg
     3241 acccggtgat gcgctgggtc gaggcgccct ggcaggacac cccctcggtg tacgg
//
LOCUS       DAOSUG010000359         3286 bp    DNA     linear   ENV 10-JUN-2024
DEFINITION  MAG TPA_asm: Micropruina sp. isolate AMR_MDS_2195
            NODE_11917_length_3286_cov_4.311978, whole genome shotgun sequence.
ACCESSION   DAOSUG010000359 DAOSUG010000000
VERSION     DAOSUG010000359.1
DBLINK      BioProject: PRJNA925477
            BioSample: SAMN32864262
            Sequence Read Archive: SRR4236673
KEYWORDS    WGS; ENV; Metagenome Assembled Genome; MAG; Third Party Data; TPA;
            TPA:assembly.
SOURCE      Micropruina sp. (wastewater metagenome)
  ORGANISM  Micropruina sp.
            Bacteria; Bacillati; Actinomycetota; Actinomycetes;
            Propionibacteriales; Nocardioidaceae; Micropruina.
REFERENCE   1  (bases 1 to 3286)
  AUTHORS   Abdulkadir,N., Saraiva,J.P., Zhang,J., Stolte,S., Gillor,O.,
            Harms,H. and Rocha,U.
  TITLE     Genome-centric analyses of 165 metagenomes show that mobile genetic
            elements are crucial for the transmission of antimicrobial
            resistance genes to pathogens in activated sludge and wastewater
  JOURNAL   Microbiol Spectr 12 (3), e0291823 (2024)
   PUBMED   38289113
REFERENCE   2  (bases 1 to 3286)
  AUTHORS   Abdulkadir,N.
  TITLE     Direct Submission
  JOURNAL   Submitted (24-MAR-2023) Environmental Microbiology, Helmholtz
            Center for Environmental Research -UFZ, Permoserstr 15, Leipzig
            04318, Germany
COMMENT     The annotation was added by the NCBI Prokaryotic Genome Annotation
            Pipeline (PGAP). Information about PGAP can be found here:
            https://www.ncbi.nlm.nih.gov/genome/annotation_prok/
            
            ##Genome-Assembly-Data-START##
            Assembly Method        :: SPAdes v. 3.11.1
            Genome Representation  :: Full
            Expected Final Version :: Yes
            Genome Coverage        :: Not Applicable
            Sequencing Technology  :: Illumina MiSeq
            ##Genome-Assembly-Data-END##
            
            ##Genome-Annotation-Data-START##
            Annotation Provider               :: NCBI
            Annotation Date                   :: 05/04/2023 04:57:10
            Annotation Pipeline               :: NCBI Prokaryotic Genome
                                                 Annotation Pipeline (PGAP)
            Annotation Method                 :: Best-placed reference protein
                                                 set; GeneMarkS-2+
            Annotation Software revision      :: 6.5
            Features Annotated                :: Gene; CDS; rRNA; tRNA; ncRNA
            Genes (total)                     :: 2,776
            CDSs (total)                      :: 2,750
            Genes (coding)                    :: 2,714
            CDSs (with protein)               :: 2,714
            Genes (RNA)                       :: 26
            tRNAs                             :: 24
            ncRNAs                            :: 2
            Pseudo Genes (total)              :: 36
            CDSs (without protein)            :: 36
            Pseudo Genes (ambiguous residues) :: 0 of 36
            Pseudo Genes (frameshifted)       :: 2 of 36
            Pseudo Genes (incomplete)         :: 32 of 36
            Pseudo Genes (internal stop)      :: 2 of 36
            ##Genome-Annotation-Data-END##
FEATURES             Location/Qualifiers
     source          1..3286
                     /organism="Micropruina sp."
                     /mol_type="genomic DNA"
                     /submitter_seqid="NODE_11917_length_3286_cov_4.311978"
                     /isolate="AMR_MDS_2195"
                     /isolation_source="Activated sludge of municipal
                     wastewater treatment plants"
                     /db_xref="taxon:2737536"
                     /environmental_sample
                     /geo_loc_name="Canada"
                     /lat_lon="49.25 N 123.1 W"
                     /collection_date="2015-06-16"
                     /metagenome_source="wastewater metagenome"
                     /note="metagenomic"
     gene            complement(27..3047)
                     /locus_tag="PLF56_11020"
     CDS             complement(27..3047)
                     /locus_tag="PLF56_11020"
                     /inference="COORDINATES: protein motif:HMM:NF033527.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_function: GO:0004803 - transposase activity [Evidence
                     IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="Tn3 family transposase"
                     /protein_id="HOQ54130.1"
                     /translation="MDEGYGRFGSYSRVELERFFHLDDEDRRLVALRRRDYNRLGFAV
                     QMVTVRHLGVFLPDPLDVPPELVEYLAEQLGIDDPSCVKRYVDRRMTRFEHADEIQRE
                     YGLAAFAAVEAELAGWIADQAWMTGDGPKAILSGAVAWLRARQALLPGITTLERLVTE
                     GRRVADQRLWSQLTGQVPASARGELLALLDAPVDGKRRASELERLRKGVFRASSKGLL
                     AAVNRLADLMAVVGALDVSAVPPRRLIGLATYGLSSKAAQLRRLEPREHRVAVLVATV
                     KVLSARATDDVLELFDLLMVTDLMSKAERESRDEKLRRYPRVSRHAGKLAAAVRVLLE
                     LTEVKPDLSLEVMWDHIENSVSKSELRAAVAVIDELVPVDDAELNGQRLEELSGRLAT
                     VRVFLPSMMGTVEFGATSDGAPVLAAMKTLAELMTAKTKLPASWLDARKVDHDLIGGG
                     WKRLVYREGRPPETVDRAAYALCLLEQFHRHLKHRNIFAPSSSRWRDPRAHLLSGEAW
                     ERTREVGMNALSLPADPGSLLADHAAALDSAYREVASRLDGDGPATVDEDGRLHLAAL
                     NAVPDSQSLVDLRRRVERMLPQVDLPELVLEVMSWVPGFAEAFTHASGNDARVADLGL
                     SVAAALCAHAMNVGFKPVTSPGVEALTRDRLHHVDQHYVRLETLTKANVALVEAQAKV
                     PLAQTWGGGLLASVDGMRFVVPVRTIHARPNPRYFGRKRGITWLNMLNDQAAGLAAKV
                     VSGTPRDSLHFIDVVAMQQGGKVPEAIITDTGSYSDIVFGLVHLGDRQYRPQLANLPD
                     QRLWRFDQNADYGPLNHAARGRIDTEKITAHWEDMCRVAVSIHSGEVSTHDVTRMISR
                     DGNPTPLGQAIAHYGRVFKTLHILRLADDEPYRREGKAQANLVEGRHDLARRIYHGKK
                     GEMVRAYYEGMEDQLSALGLVLNCVVLWNTVYCNRALDQLRDQGYPVLDTDAERLSAF
                     IRDHIGIDGHYAFHLPDLGGTHRPLRDPDSSDDD"
BASE COUNT          522 a         1185 c         1072 g          507 t
ORIGIN      
        1 ccccttgatc tactgccgca ctccagtcag tcgtcgtcgg agctgtccgg gtcgcgcagc
       61 gggcggtggg tgccgccgag gtcgggcagg tggaaggcgt agtgcccgtc gatgccaatg
      121 tggtcacgga tgaacgccga cagtcgttcc gcgtcggtgt cgaggaccgg atagccctgg
      181 tcgcggagtt ggtcgagggc gcggttgcag tagacggtgt tccacagcac cacgcagttg
      241 agcaccagcc cgagcgcgga caactggtcc tccatgccct cgtagtaggc gcgcaccatc
      301 tcacccttct tgccgtgata gatccgccgc gccaggtcgt ggcgcccctc cacgaggttg
      361 gcctgcgcct ttccctcgcg ccggtagggt tcgtcgtcgg ccaggcgcag gatgtgcagg
      421 gtcttgaaga cccgcccgta gtgggcgatg gcctgcccca acggcgtcgg gttaccgtcg
      481 cgggagatca tgcgggtgac gtcgtgggtg gagacctcgc cggagtggat cgacaccgcg
      541 acccggcaca tgtcctccca gtgggcggtg atcttctcgg tgtcgatgcg gccgcgtgcg
      601 gcgtggttga gcggcccgta gtcggcgttc tgatcgaaac gccacagccg ctggtcgggc
      661 aggttcgcca gctgcggccg gtattgccgg tcgccgaggt ggaccagtcc gaacacgatg
      721 tcggagtaag agccggtatc ggtgatgatc gcttcgggca ccttgccgcc ctgctgcatc
      781 gccacgacgt cgatgaagtg cagagagtcg cgcggcgtgc ccgacaccac cttggccgcc
      841 aaccctgccg cctggtcgtt gagcatgttc aaccaggtga tgccgcgctt acggccgaag
      901 tacctcgggt tcggtctggc gtggatcgtg cggaccggga ccacgaaccg catgccgtcc
      961 accgaagcca gcagcccgcc gccccaggtc tgcgccaacg gcaccttggc ctgggcctcg
     1021 accagcgcga cgttggcctt ggtcagcgtc tccagacgca cgtagtgctg gtcgacgtgg
     1081 tggaggcggt cacgggtcaa cgcctccaca ccgggactgg tcaccggttt gaagcccacg
     1141 ttcatcgcgt gcgcgcacag cgccgcggcg acggacaacc cgagatcggc gacgcgggcg
     1201 tcgttcccgg aggcgtgggt gaacgcctcg gcgaacccgg gcacccacga catcacctcc
     1261 agcaccagct cgggcaggtc gacctgcggg agcatccgct cgacccggcg gcgcagatcg
     1321 accagcgact gcgaatcggg gaccgcgttg agcgcggcca ggtgcagcct gccgtcctcg
     1381 tcgacggtcg ccgggccgtc cccgtccagt cgtgaagcga cctcgcggta ggcgctatcc
     1441 agggcggccg catggtcggc gagcagcgat cccgggtccg caggcaggct cagcgcgttc
     1501 atgcccacct cgcgggtccg ctcccacgcc tcgccggaca gcaggtgagc ccgcggatcg
     1561 cgccacctcg aggaggacgg ggcgaagatg ttgcggtgct tgaggtgccg atggaactgt
     1621 tccagcaggc acagcgcgta ggcggcccgg tccacggtct ccggcgggcg accctcccgg
     1681 tagaccaggc gtttccagcc gccgccgatc aggtcgtggt cgaccttgcg ggcgtccaac
     1741 cagctcgcgg gcagcttcgt cttggccgtc atcaactccg ccagcgtctt catcgcggcc
     1801 aacaccgggg caccgtcgga ggtggccccg aactcgacgg tgcccatcat cgacggcagg
     1861 aagacccgga ccgtcgccaa ccggcccgac agctcctcca ggcgttgccc gttcagctcg
     1921 gcgtcgtcga ccgggaccaa ctcgtcgatg accgcgaccg cggcccgcag ctcgctcttg
     1981 gagacggaat tctcgatgtg atcccacatc acctccaagc tgagatccgg cttcacctcg
     2041 gtcagctcca gcagcaccct caccgcggcg gccagtttgc ccgcgtggcg agacaccctc
     2101 ggataccggc gcagcttctc gtcgcgggac tcccgttcgg ccttcgacat caggtcggtg
     2161 accatcagca ggtcgaacag ctcgagtacg tcgtccgtcg cccgcgctga gagcaccttc
     2221 accgtcgcga ccaacaccgc cacacggtgt tcgcgcggtt cgaggcgacg cagctgcgcc
     2281 gccttgctcg acaacccgta ggtggccagc ccgatcagcc gtcgcggcgg aaccgccgaa
     2341 acatccagcg ccccgacgac cgccatcagg tcggccaacc ggttcacggc cgccagcagg
     2401 cccttcgagc tggcgcggaa cacccccttg cgcagccgtt ccagctcgct ggcccggcgt
     2461 tttccgtcca ccggcgcatc cagcagcgcc agcagctctc cccgcgccga cgccggaacc
     2521 tggccggtga gctgggacca caaccgctgg tcggcgaccc tgcggccctc ggtgaccagc
     2581 cgctccaggg tcgtgatgcc cggcaacagg gcctggcggg cgcgtagcca ggccaccgcc
     2641 ccggacaaga tggccttcgg cccgtcgccg gtcatccacg cctggtcggc gatccagccg
     2701 gccagctccg cctccacggc ggcgaacgcg gccaggccgt actcccgctg gatctcgtcc
     2761 gcgtgctcga agcgagtcat ccgccgatcg acgtaacgct tcacacacga cgggtcgtcg
     2821 atgcccagct gctcggccaa gtactcgacc agctcgggcg gaacgtccaa cgggtcgggc
     2881 aggaacacgc ccaggtggcg caccgtgacc atctgcacgg cgaagccgag ccggttgtag
     2941 tcgcggcgac gcaacgcaac cagccgtcga tcctcgtcgt ccaagtggaa gaaccgctcc
     3001 aactccaccc gcgagtacga accgaaccgg ccgtaaccct cgtccatcac cgcgacatga
     3061 gatcaccgac cagcgcaacg ccgctcaccc cgagtcgatc caccgaacga cgccgacacc
     3121 gcgaccggtc tcacggcgaa ccttccctta gcgctacttt tcccggagat gttcctgcac
     3181 tgccggcttt gccgccgccg gtagagccga accacttctg gaaaagtcgg aaagcgttgc
     3241 ggttgccgta gctggagcag gcgtcgccct cgtccgggta ggcagc
//
LOCUS       DAOSUG010000360         3278 bp    DNA     linear   ENV 10-JUN-2024
DEFINITION  MAG TPA_asm: Micropruina sp. isolate AMR_MDS_2195
            NODE_11962_length_3278_cov_4.013031, whole genome shotgun sequence.
ACCESSION   DAOSUG010000360 DAOSUG010000000
VERSION     DAOSUG010000360.1
DBLINK      BioProject: PRJNA925477
            BioSample: SAMN32864262
            Sequence Read Archive: SRR4236673
KEYWORDS    WGS; ENV; Metagenome Assembled Genome; MAG; Third Party Data; TPA;
            TPA:assembly.
SOURCE      Micropruina sp. (wastewater metagenome)
  ORGANISM  Micropruina sp.
            Bacteria; Bacillati; Actinomycetota; Actinomycetes;
            Propionibacteriales; Nocardioidaceae; Micropruina.
REFERENCE   1  (bases 1 to 3278)
  AUTHORS   Abdulkadir,N., Saraiva,J.P., Zhang,J., Stolte,S., Gillor,O.,
            Harms,H. and Rocha,U.
  TITLE     Genome-centric analyses of 165 metagenomes show that mobile genetic
            elements are crucial for the transmission of antimicrobial
            resistance genes to pathogens in activated sludge and wastewater
  JOURNAL   Microbiol Spectr 12 (3), e0291823 (2024)
   PUBMED   38289113
REFERENCE   2  (bases 1 to 3278)
  AUTHORS   Abdulkadir,N.
  TITLE     Direct Submission
  JOURNAL   Submitted (24-MAR-2023) Environmental Microbiology, Helmholtz
            Center for Environmental Research -UFZ, Permoserstr 15, Leipzig
            04318, Germany
COMMENT     The annotation was added by the NCBI Prokaryotic Genome Annotation
            Pipeline (PGAP). Information about PGAP can be found here:
            https://www.ncbi.nlm.nih.gov/genome/annotation_prok/
            
            ##Genome-Assembly-Data-START##
            Assembly Method        :: SPAdes v. 3.11.1
            Genome Representation  :: Full
            Expected Final Version :: Yes
            Genome Coverage        :: Not Applicable
            Sequencing Technology  :: Illumina MiSeq
            ##Genome-Assembly-Data-END##
            
            ##Genome-Annotation-Data-START##
            Annotation Provider               :: NCBI
            Annotation Date                   :: 05/04/2023 04:57:10
            Annotation Pipeline               :: NCBI Prokaryotic Genome
                                                 Annotation Pipeline (PGAP)
            Annotation Method                 :: Best-placed reference protein
                                                 set; GeneMarkS-2+
            Annotation Software revision      :: 6.5
            Features Annotated                :: Gene; CDS; rRNA; tRNA; ncRNA
            Genes (total)                     :: 2,776
            CDSs (total)                      :: 2,750
            Genes (coding)                    :: 2,714
            CDSs (with protein)               :: 2,714
            Genes (RNA)                       :: 26
            tRNAs                             :: 24
            ncRNAs                            :: 2
            Pseudo Genes (total)              :: 36
            CDSs (without protein)            :: 36
            Pseudo Genes (ambiguous residues) :: 0 of 36
            Pseudo Genes (frameshifted)       :: 2 of 36
            Pseudo Genes (incomplete)         :: 32 of 36
            Pseudo Genes (internal stop)      :: 2 of 36
            ##Genome-Annotation-Data-END##
FEATURES             Location/Qualifiers
     source          1..3278
                     /organism="Micropruina sp."
                     /mol_type="genomic DNA"
                     /submitter_seqid="NODE_11962_length_3278_cov_4.013031"
                     /isolate="AMR_MDS_2195"
                     /isolation_source="Activated sludge of municipal
                     wastewater treatment plants"
                     /db_xref="taxon:2737536"
                     /environmental_sample
                     /geo_loc_name="Canada"
                     /lat_lon="49.25 N 123.1 W"
                     /collection_date="2015-06-16"
                     /metagenome_source="wastewater metagenome"
                     /note="metagenomic"
     gene            complement(<1..265)
                     /locus_tag="PLF56_11025"
     CDS             complement(<1..265)
                     /locus_tag="PLF56_11025"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_015069554.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_process: GO:0009401 - phosphoenolpyruvate-dependent
                     sugar phosphotransferase system [Evidence IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="HPr family phosphocarrier protein"
                     /protein_id="HOQ54131.1"
                     /translation="MATREVAIGSSVGLHARPASIFVQAATATGLPVTIAKQGGNPVD
                     ARSILGVMALGAKNGEVVTLSAEGDGADAALDSLVALLERDLDA"
     gene            554..1312
                     /locus_tag="PLF56_11030"
     CDS             554..1312
                     /locus_tag="PLF56_11030"
                     /inference="COORDINATES: protein motif:HMM:NF012422.2"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_process: GO:0006355 - regulation of DNA-templated
                     transcription [Evidence IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="LuxR C-terminal-related transcriptional
                     regulator"
                     /protein_id="HOQ54132.1"
                     /translation="MLQVDAAEGAVEPAVIVLDMLCEAMHALTLGRSADEIVCRSLGR
                     ALNADAAVTIAVQPDAAPYVAASYPRTERVRAFAADLLHGELVAGQEQFVHHYVESVG
                     HVAALSMQASDVLPSAGHGRVLVYVRATPFDAGDRLLMDRARDALVALWPHAERSVRA
                     VEAQQWVRDKVDDSSMTERELQVLGLLAEGLLATSIASRLRLSPRTVHKHLGNIYRKL
                     GVHDRLVAVSLARLQGLVSGYPTSAASPVRAEAG"
     gene            complement(1320..2615)
                     /gene="clpX"
                     /locus_tag="PLF56_11035"
     CDS             complement(1320..2615)
                     /gene="clpX"
                     /locus_tag="PLF56_11035"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_012919918.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_function: GO:0005524 - ATP binding [Evidence IEA];
                     GO_function: GO:0008270 - zinc ion binding [Evidence IEA];
                     GO_function: GO:0016887 - ATP hydrolysis activity
                     [Evidence IEA];
                     GO_function: GO:0046983 - protein dimerization activity
                     [Evidence IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="ATP-dependent Clp protease ATP-binding subunit
                     ClpX"
                     /protein_id="HOQ54133.1"
                     /translation="MARIGETSELLKCSFCGKSQKQVKKLIAGPGVYICDECIDLCNE
                     IIEEEFSEAADVGLLDDLPKPREICSFLDSYVIGQDVAKKALSVAVYNHYKRVQAQAN
                     TPPNRRSAEEESVELGKSNILLIGPTGCGKTYLAQTLARMLNVPFAMADATALTEAGY
                     VGEDVENILLKLIQAADFDVKKAETGIIYIDEIDKVARKSENPSITRDVSGEGVQQAL
                     LKILEGTTASVPPQGGRKHPHQDFIQIDTTNILFIVGGAFAGLEEIINARIGKRPLGF
                     NNDAAVRKAASNNPFEEVRPEDLHRYGLIPEFIGRLPMISTVASLDSTSLVRILVEPK
                     NALIKQFRKLFEMDGVDLQFTETAIAAVAEMALHRGIGARGLRSILEEILLNVMYHVP
                     SDDDVARVLVTEEVVRDGVAPTLVSRAQVARSDRREKSA"
     gene            complement(2691..>3278)
                     /locus_tag="PLF56_11040"
     CDS             complement(2691..>3278)
                     /locus_tag="PLF56_11040"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_014846845.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="ATP-dependent Clp protease proteolytic subunit"
                     /protein_id="HOQ54134.1"
                     /translation="PQWEERTSYGVRRVDPYTKLFEDRIIFLGTPISDDIANAVMAQL
                     LCLQSMDPERPISIYLNSPGGSFTALTAIYDTMRYIKPDVQTVCLGQAASAAAVLLAA
                     GTKGKRLALPNSRILIHQPATEGGYGQSSDLEIQAREILRIRSLMEGMLAESTGQSVD
                     KISRDIERDKYLTAEEAVEYGIVDAILTSLKDLDA"
BASE COUNT          516 a         1044 c         1109 g          609 t
ORIGIN      
        1 cggcgtccag atcccgctcg agcagcgcga ccagcgagtc gagcgcggcg tccgcgccgt
       61 cgccttctgc ggacaaggtg acgacctcgc cgttcttggc acccagggcc atcacgccca
      121 ggatgctgcg ggcatcgaca gggttgccgc cctgcttggc gatggtgacc ggaaggccgg
      181 tcgctgtggc ggcctggacg aagatggacg cgggacgggc gtgcaggcca acggacgaac
      241 cgatagcaac ttcacgggtg gccatgaatg actcctttgt gcgaaatggc ccagtggacc
      301 tgggtgatga ccgggcgggg tgttcggttg gtcgagcggc gcggtcgctg ccaatatatg
      361 gcggttcgcc aggggccatc aagcgaaacg cagttctggg cacgcttgca gacgaagcca
      421 agggtccgga aggtcttgtt cgtgaatacg cgcgtgtgcg acggtatttc ccgagtacca
      481 cacagctcct gaccggtaca cgtcaggagt tcgaatgatt gattcttgca ctatcgcccg
      541 aaggggcccg cgcgtgttgc aggtcgacgc agctgagggg gctgtcgagc ccgccgtcat
      601 cgtgctcgac atgctctgtg aagccatgca tgccctcact cttggacgct ccgccgacga
      661 gatcgtgtgt cgctcgctgg gtcgcgcgct gaacgctgac gccgcggtca cgattgcggt
      721 ccagccggac gcggcaccct acgtcgcagc cagctacccg cgcaccgagc gggtgcgggc
      781 ctttgccgcc gacctgctgc acggcgagct ggtggcgggc caggaacagt tcgttcatca
      841 ctacgtcgaa tcggtcggcc acgtggcggc gttgtcgatg caggccagcg acgtgctgcc
      901 ctcggccggg catggccgcg tgctggtcta cgtgcgtgct acccccttcg acgccggcga
      961 ccgcctgctg atggatcgcg cccgcgacgc gctggtggcg ttgtggccgc acgcggagcg
     1021 ctcggtgcgg gcggtcgagg cccagcaatg ggtgcgtgac aaggtggacg attcgtccat
     1081 gaccgaacgc gagctgcagg tgctgggcct gctggccgag ggtctgttgg ccacctcgat
     1141 cgcctcacgc cttcgactgt cgccgcgcac ggtgcacaag catctgggca acatctaccg
     1201 caagctcggg gtgcacgacc gcctggtggc ggtcagcctg gcccggctgc aggggctggt
     1261 cagcggctac ccgaccagcg cagccagtcc ggttcgtgcc gaggccggct gatcgggtct
     1321 caggcgctct tctcgcgccg gtcgctgcgg gccacctggg cccgcgacac caacgtgggc
     1381 gcgacaccgt cgcgcaccac ctcttcggtg accagcaccc gggccacgtc atcgtcgctc
     1441 ggcacgtgat acatcacgtt gagcagtatc tcttcgagga tcgagcggag cccgcgggcg
     1501 ccgatgccgc ggtgcagcgc catctctgcc accgcggcga tcgccgtctc ggtgaactgc
     1561 aggtcgaccc cgtccatctc gaagagcttg cggaactgct tgatcagcgc gttcttgggc
     1621 tcgaccagaa tgcgcaccag cgaggtggag tcgagtgagg ccaccgtgct gatcatcggc
     1681 aaccggccga tgaactcggg gatcagcccg taccggtgca ggtcttcggg acgcacctct
     1741 tcaaacgggt tgttggacgc cgccttgcgc accgccgcgt cgttgttgaa ccccagcggg
     1801 cgcttgccga tgcgtgcatt gatgatctct tcgagtccgg cgaaggcgcc accgacgatg
     1861 aacaggatgt tcgtggtgtc gatctggatg aagtcctggt gcggatgctt gcgcccaccc
     1921 tgcgggggga cggaggccgt ggtgccctcg agaatcttca gcagcgcctg ctggacgccc
     1981 tcgccggaca cgtcgcgggt gatcgacggg ttctcactct tgcgagccac cttgtcgatc
     2041 tcatcgatgt aaatgatgcc ggtctcggcc ttcttgacgt caaaatctgc ggcctgaatg
     2101 agcttgagca gaatgttctc tacgtcttcg cccacatagc ccgcttcggt cagcgcggtg
     2161 gcgtcggcca tggcgaacgg cacgttgagc atgcgggcca gggtttgcgc gaggtaggtc
     2221 ttgccacaac ccgtagggcc gatcagcaga atgttggact tgcccagctc gaccgactct
     2281 tcttcggccg agcgccggtt cggcggggtg ttcgcctggg cctggacgcg cttgtagtgg
     2341 ttgtagacgg ccacgctgag ggccttcttg gctacgtcct gaccgatcac gtacgagtcg
     2401 aggaatgagc agatctcgcg cggcttgggc aggtcatcga gcaggccgac gtcggcggcc
     2461 tccgagaact cctcctcgat gatctcgttg cagagatcga tgcactcgtc gcagatgtag
     2521 acacccggac ccgcgatcaa cttcttgacc tgcttttggc tctttccgca gaacgagcat
     2581 ttgagcagct ccgaggtctc tccgattcgc gccacgaccc ctcccttccg tgcaggccac
     2641 actagcccgc cagggcgcat ccgcaaggga cgcgccccag cgcgaccagg tcaggcgtcg
     2701 aggtctttga gtgaggtcag aatcgcatcg acgatgccgt actcgaccgc ctcttcggcg
     2761 gtcaggtact tgtcacgctc gatgtcgcgg ctgatcttgt cgacgctctg gccggtggac
     2821 tcggccagca tgccctccat cagcgaacgg atgcgaagaa tttcacgggc ctggatctcg
     2881 aggtcggagc tctggccgta gccgccctcg gtggccggct gatgaatcag gatgcggctg
     2941 ttgggcagcg ccaaccgctt gcccttggtg ccggcggcga gcaacaccgc ggcggccgag
     3001 gccgcctggc ccaggcacac ggtctgcacg tcgggcttga tgtagcgcat cgtgtcgtag
     3061 atcgcggtga gcgccgtgaa cgaaccgccg gggctgttca ggtagatgct gatcggacgc
     3121 tcgggatcca tcgactgcag gcacagcaac tgcgccatca cggcgttggc gatgtcgtcg
     3181 ctgatcgggg tgccgaggaa gatgatgcgg tcctcgaaca gcttggtgta ggggtcgacg
     3241 cgccggacgc cgtagctggt gcgctcctcc cactgcgg
//
LOCUS       DAOSUG010000361         3276 bp    DNA     linear   ENV 10-JUN-2024
DEFINITION  MAG TPA_asm: Micropruina sp. isolate AMR_MDS_2195
            NODE_11974_length_3276_cov_4.324123, whole genome shotgun sequence.
ACCESSION   DAOSUG010000361 DAOSUG010000000
VERSION     DAOSUG010000361.1
DBLINK      BioProject: PRJNA925477
            BioSample: SAMN32864262
            Sequence Read Archive: SRR4236673
KEYWORDS    WGS; ENV; Metagenome Assembled Genome; MAG; Third Party Data; TPA;
            TPA:assembly.
SOURCE      Micropruina sp. (wastewater metagenome)
  ORGANISM  Micropruina sp.
            Bacteria; Bacillati; Actinomycetota; Actinomycetes;
            Propionibacteriales; Nocardioidaceae; Micropruina.
REFERENCE   1  (bases 1 to 3276)
  AUTHORS   Abdulkadir,N., Saraiva,J.P., Zhang,J., Stolte,S., Gillor,O.,
            Harms,H. and Rocha,U.
  TITLE     Genome-centric analyses of 165 metagenomes show that mobile genetic
            elements are crucial for the transmission of antimicrobial
            resistance genes to pathogens in activated sludge and wastewater
  JOURNAL   Microbiol Spectr 12 (3), e0291823 (2024)
   PUBMED   38289113
REFERENCE   2  (bases 1 to 3276)
  AUTHORS   Abdulkadir,N.
  TITLE     Direct Submission
  JOURNAL   Submitted (24-MAR-2023) Environmental Microbiology, Helmholtz
            Center for Environmental Research -UFZ, Permoserstr 15, Leipzig
            04318, Germany
COMMENT     The annotation was added by the NCBI Prokaryotic Genome Annotation
            Pipeline (PGAP). Information about PGAP can be found here:
            https://www.ncbi.nlm.nih.gov/genome/annotation_prok/
            
            ##Genome-Assembly-Data-START##
            Assembly Method        :: SPAdes v. 3.11.1
            Genome Representation  :: Full
            Expected Final Version :: Yes
            Genome Coverage        :: Not Applicable
            Sequencing Technology  :: Illumina MiSeq
            ##Genome-Assembly-Data-END##
            
            ##Genome-Annotation-Data-START##
            Annotation Provider               :: NCBI
            Annotation Date                   :: 05/04/2023 04:57:10
            Annotation Pipeline               :: NCBI Prokaryotic Genome
                                                 Annotation Pipeline (PGAP)
            Annotation Method                 :: Best-placed reference protein
                                                 set; GeneMarkS-2+
            Annotation Software revision      :: 6.5
            Features Annotated                :: Gene; CDS; rRNA; tRNA; ncRNA
            Genes (total)                     :: 2,776
            CDSs (total)                      :: 2,750
            Genes (coding)                    :: 2,714
            CDSs (with protein)               :: 2,714
            Genes (RNA)                       :: 26
            tRNAs                             :: 24
            ncRNAs                            :: 2
            Pseudo Genes (total)              :: 36
            CDSs (without protein)            :: 36
            Pseudo Genes (ambiguous residues) :: 0 of 36
            Pseudo Genes (frameshifted)       :: 2 of 36
            Pseudo Genes (incomplete)         :: 32 of 36
            Pseudo Genes (internal stop)      :: 2 of 36
            ##Genome-Annotation-Data-END##
FEATURES             Location/Qualifiers
     source          1..3276
                     /organism="Micropruina sp."
                     /mol_type="genomic DNA"
                     /submitter_seqid="NODE_11974_length_3276_cov_4.324123"
                     /isolate="AMR_MDS_2195"
                     /isolation_source="Activated sludge of municipal
                     wastewater treatment plants"
                     /db_xref="taxon:2737536"
                     /environmental_sample
                     /geo_loc_name="Canada"
                     /lat_lon="49.25 N 123.1 W"
                     /collection_date="2015-06-16"
                     /metagenome_source="wastewater metagenome"
                     /note="metagenomic"
     gene            <1..248
                     /locus_tag="PLF56_11045"
     CDS             <1..248
                     /locus_tag="PLF56_11045"
                     /inference="COORDINATES: ab initio
                     prediction:GeneMarkS-2+"
                     /note="Derived by automated computational analysis using
                     gene prediction method: GeneMarkS-2+."
                     /codon_start=3
                     /transl_table=11
                     /product="hypothetical protein"
                     /protein_id="HOQ54135.1"
                     /translation="PSQAGYPQQPYGQPPAQQAPYGQQQTYGQPQAYGQAPTQDSPQQ
                     APSQPSAESYDRGEGIGRAPSSDHQNEQDGRTPRGDN"
     gene            complement(324..737)
                     /locus_tag="PLF56_11050"
     CDS             complement(324..737)
                     /locus_tag="PLF56_11050"
                     /inference="COORDINATES: protein motif:HMM:NF018028.2"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="RDD family protein"
                     /protein_id="HOQ54136.1"
                     /translation="MLATWGRRALGGLIDFVAPEIVVSMIIGLIFADRTSSVANLLST
                     VLTLGFWCYNTGYRLATTGYSFGHKIAQVRVVMADSGQTPTVNTAVLRAVCHLLDSWA
                     CMIGWLFPLWDAKKQTFADKITKTVSLDERNAGQR"
     gene            852..2582
                     /locus_tag="PLF56_11055"
     CDS             852..2582
                     /locus_tag="PLF56_11055"
                     /inference="COORDINATES: protein motif:HMM:NF016408.2"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="PKD domain-containing protein"
                     /protein_id="HOQ54137.1"
                     /translation="MNYSIRKADRIIGVLADEAGFEYVNMDDVLRGHEQCTEEEAMNG
                     IKGVIWATDPGSYHPNRLGHELMADRIQGQIELDEPSTHIIGPNVTLTQKFTFSGRRV
                     TVNVGWPGSHVVTTLISPSGVRYTRDDARDAAHTNGPTWETFTIEDPEPGEWTIEMFG
                     ADVDPEGEPVDLVVTDTPDWNAEPVADINVTRIDDDTYRFDATGSTDADGEITGYEWD
                     FGDSVTMVPGAVVEHTFAPGDFKVALVVTDDDGGLDLTTTEGTITVAEPNRASDIVIR
                     SGTQFTNQLQITGDTFIDGNLQCNSAATIDGRLTVAGTLHLTNDCAIEGDVIATGDVT
                     LDSRSSITGDLTSAGSIQMQSSSTVTGTIAVTGALTIIDGVSVDALIASGHIGAVELQ
                     HPVLTVTGPVQEATEVATTTMWRHWLNTLARQSGAPPWSPGLSASPGCTIAPWTINAA
                     DVVVTESLAIDARRTATGCSAIQIQGTSLTLSSDLSLYVDSFAASNGLRVRSADGDAH
                     RLRIIVTDGVPSCANNTIRLIGDTDIDPLINLDLTTPGGVNIHGNASLNARIDAGCFA
                     TSGKVRLGVS"
     gene            2572..2928
                     /locus_tag="PLF56_11060"
     CDS             2572..2928
                     /locus_tag="PLF56_11060"
                     /inference="COORDINATES: ab initio
                     prediction:GeneMarkS-2+"
                     /note="Derived by automated computational analysis using
                     gene prediction method: GeneMarkS-2+."
                     /codon_start=1
                     /transl_table=11
                     /product="hypothetical protein"
                     /protein_id="HOQ54138.1"
                     /translation="MSADPPRSRSRARNALHLLWVLPLAGVLSMFPIALASFSLCGIG
                     CYSPGFIRYEELPLAVLMCAIGGLIVFLAVQLVPWTQRAWTRLLVSIPAGLLWGGIWL
                     LLSLHAFDPMSPIFGR"
     gene            complement(2925..>3276)
                     /locus_tag="PLF56_11065"
     CDS             complement(2925..>3276)
                     /locus_tag="PLF56_11065"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_076612349.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_process: GO:0015074 - DNA integration [Evidence IEA]"
                     /codon_start=2
                     /transl_table=11
                     /product="integrase core domain-containing protein"
                     /protein_id="HOQ54139.1"
                     /translation="FWTRANAFFASLGVTVTAVMTDNGACYRSHAFADALGDGVKHKW
                     TKPYRPQTNGKVERFNRTLAAEWAYAKPYASEAERAAAYETWLHHYNHHRPHTGIGGQ
                     TPSARVHNVTGKYI"
BASE COUNT          587 a         1100 c         1049 g          540 t
ORIGIN      
        1 atccgtcaca ggccggttac ccccagcagc cctacgggca gccgccggcc cagcaggcgc
       61 cgtacggtca gcagcagacc tacgggcaac cgcaggccta cggccaagcc cccacgcagg
      121 actcgccgca gcaggcgccc agccaaccct cggccgagtc gtacgaccgg ggcgagggca
      181 tcggccgggc accgtcgtcc gatcaccaga acgagcagga cggacgcacc ccgcgcggcg
      241 acaactgagc ctcgatcgtc tggtcaggcg aagcgaaagg gccgcccccg gttgggggcg
      301 gccctttctt gggttcgggt ggctcagcgc tgcccggcgt tgcgctcgtc gagggagaca
      361 gtcttggtga tcttgtcggc gaacgtctgc ttcttggcgt cccacaaggg gaacagccag
      421 ccgatcatgc aggcccacga gtcgagcagg tggcacaccg cacgcagcac ggccgtgttg
      481 accgtcggag tctggccgct gtcggccatc accacgcgca cctgggcgat cttgtggccg
      541 aacgagtagc cggtggtggc gaggcggtaa ccggtgttat agcaccagaa accgagcgtg
      601 agcacggtgc tcagcaggtt cgccacggat gacgtgcggt cggcaaagat cagtccgatg
      661 atcatcgaga ccacgatctc tggagcgacg aagtcgatca ggccgcccag agcgcgacgt
      721 ccccaggtgg cgagcaccgg gcccgagggg gtataggggt tgggctggta gccaccgggt
      781 gcaccgtacg gcgaaggggc cccgtagccg gcgttgggag ccccgtaggg ggtctgggca
      841 cccaggcatg gatgaactat tcgatccgca aagccgaccg tatcatcggg gttctcgccg
      901 acgaagccgg gttcgagtac gtcaacatgg atgatgtcct ccgcgggcac gagcagtgta
      961 ccgaggaaga ggctatgaac gggatcaagg gtgtgatctg ggcgaccgac cccggttctt
     1021 accacccgaa ccgactcggc cacgagctca tggccgaccg tattcagggg cagatcgaac
     1081 tcgacgaacc gtcgacccac atcatcggac cgaacgtcac tctgacgcag aagttcacct
     1141 tctcggggcg cagggtcact gtcaacgtcg gatggccggg cagccatgtc gtcacgaccc
     1201 tgatctcacc ttcgggtgtg cgctacaccc gtgacgacgc acgggatgcc gcacacacga
     1261 acgggccgac atgggagacc ttcacgattg aggaccccga gcccggcgag tggacgatcg
     1321 agatgttcgg cgctgatgtt gaccccgagg gcgagccggt ggacctcgta gtcaccgaca
     1381 ctccggactg gaacgccgag ccggtcgctg acatcaatgt cacccggatc gacgacgaca
     1441 cctaccgctt cgatgcgacc ggttccaccg acgccgatgg cgagatcacg ggctacgagt
     1501 gggacttcgg tgacagcgtg accatggtcc cgggcgccgt cgtcgagcac accttcgcgc
     1561 cgggcgactt caaggtcgcg ctggtggtca ccgatgacga cggcgggctc gacctcacca
     1621 cgaccgaagg gaccatcacg gtcgccgaac cgaatcgtgc ctccgacatc gtcatccgat
     1681 cagggacgca gttcaccaat cagctgcaga tcactggcga caccttcatc gacggaaacc
     1741 tgcaatgcaa cagcgcagcc acgatcgacg ggcggctcac ggtggctggc accctccacc
     1801 tcaccaacga ttgcgcgatc gaaggcgacg tgatcgcgac cggagatgtc accctcgaca
     1861 gccggagcag catcaccggc gacctcacct ccgccgggtc gatccagatg cagtccagct
     1921 ccacggtcac cggaaccatc gcagtcaccg gcgcactcac aatcatcgac ggtgtcagcg
     1981 tcgacgcgct catcgcgtcg ggacacatcg gggccgtcga gctccaacat cctgtcctga
     2041 ctgtcaccgg gccggtgcag gaggcgactg aggtcgcgac gacgaccatg tggcggcatt
     2101 ggctgaacac cctcgcccgg cagagtggcg caccgccgtg gagcccaggg ctgtctgcct
     2161 cgcccggttg caccatcgcc ccctggacaa tcaatgccgc agacgtggtg gtcaccgagt
     2221 ctctggcgat cgacgcgcgc cgcacggcga cgggatgctc ggccatccag atccaaggca
     2281 catctctcac gctctcgtcc gacctgtccc tttacgtcga ctcgttcgcc gccagcaacg
     2341 gactgcgcgt gcgctccgcg gatggcgacg cacaccggtt gcgaatcatc gtcaccgatg
     2401 gcgtcccgtc ctgcgcgaac aacacgatcc gcctcatcgg cgacacggac atcgacccgc
     2461 tcatcaacct cgacctgaca actcccggtg gcgtgaacat tcacggaaac gcgtcgctga
     2521 acgcccgaat cgatgccggc tgcttcgcga cgagcggaaa ggtgagactg ggtgtcagct
     2581 gatccaccgc gcagccggag tcgggcgcgc aacgccctcc atctgctctg ggtactccct
     2641 ctcgccgggg tgctgtcgat gttccccatc gcactcgcat cgttcagcct ctgcgggatc
     2701 ggctgctact cacccgggtt catccgatac gaagagttgc cgctggcggt cctgatgtgc
     2761 gcgatcggcg gactcatagt gttcctcgcc gtgcagctcg tcccgtggac acagcgcgcc
     2821 tggaccagac tgctcgtctc catacccgcg ggcctgcttt ggggaggtat ctggctcctg
     2881 ctcagcctcc acgcgttcga cccgatgtcg cccatcttcg gacgctagat gtacttcccc
     2941 gtgacgttgt gaacgcgggc tgagggggtc tggccgccga tcccggtgtg gggtctgtgg
     3001 tgattgtagt gatgcagcca ggtctcgtag gccgcggctc gctctgcttc gctggcgtag
     3061 ggcttggcgt aggcccattc ggcggcgagg gtgcggttga agcgttcgac cttcccgttg
     3121 gtctgcggcc gatacggctt tgtccacttg tgcttcacac cgtcgccgag cgcgtccgcg
     3181 aacgcgtgtg accggtagca cgcgccgttg tctgtcatca cggcggtgac cgtgactccg
     3241 aggctcgcga agaacgcgtt cgcgcgggtc cagaac
//
LOCUS       DAOSUG010000362         3273 bp    DNA     linear   ENV 10-JUN-2024
DEFINITION  MAG TPA_asm: Micropruina sp. isolate AMR_MDS_2195
            NODE_11985_length_3273_cov_4.376942, whole genome shotgun sequence.
ACCESSION   DAOSUG010000362 DAOSUG010000000
VERSION     DAOSUG010000362.1
DBLINK      BioProject: PRJNA925477
            BioSample: SAMN32864262
            Sequence Read Archive: SRR4236673
KEYWORDS    WGS; ENV; Metagenome Assembled Genome; MAG; Third Party Data; TPA;
            TPA:assembly.
SOURCE      Micropruina sp. (wastewater metagenome)
  ORGANISM  Micropruina sp.
            Bacteria; Bacillati; Actinomycetota; Actinomycetes;
            Propionibacteriales; Nocardioidaceae; Micropruina.
REFERENCE   1  (bases 1 to 3273)
  AUTHORS   Abdulkadir,N., Saraiva,J.P., Zhang,J., Stolte,S., Gillor,O.,
            Harms,H. and Rocha,U.
  TITLE     Genome-centric analyses of 165 metagenomes show that mobile genetic
            elements are crucial for the transmission of antimicrobial
            resistance genes to pathogens in activated sludge and wastewater
  JOURNAL   Microbiol Spectr 12 (3), e0291823 (2024)
   PUBMED   38289113
REFERENCE   2  (bases 1 to 3273)
  AUTHORS   Abdulkadir,N.
  TITLE     Direct Submission
  JOURNAL   Submitted (24-MAR-2023) Environmental Microbiology, Helmholtz
            Center for Environmental Research -UFZ, Permoserstr 15, Leipzig
            04318, Germany
COMMENT     The annotation was added by the NCBI Prokaryotic Genome Annotation
            Pipeline (PGAP). Information about PGAP can be found here:
            https://www.ncbi.nlm.nih.gov/genome/annotation_prok/
            
            ##Genome-Assembly-Data-START##
            Assembly Method        :: SPAdes v. 3.11.1
            Genome Representation  :: Full
            Expected Final Version :: Yes
            Genome Coverage        :: Not Applicable
            Sequencing Technology  :: Illumina MiSeq
            ##Genome-Assembly-Data-END##
            
            ##Genome-Annotation-Data-START##
            Annotation Provider               :: NCBI
            Annotation Date                   :: 05/04/2023 04:57:10
            Annotation Pipeline               :: NCBI Prokaryotic Genome
                                                 Annotation Pipeline (PGAP)
            Annotation Method                 :: Best-placed reference protein
                                                 set; GeneMarkS-2+
            Annotation Software revision      :: 6.5
            Features Annotated                :: Gene; CDS; rRNA; tRNA; ncRNA
            Genes (total)                     :: 2,776
            CDSs (total)                      :: 2,750
            Genes (coding)                    :: 2,714
            CDSs (with protein)               :: 2,714
            Genes (RNA)                       :: 26
            tRNAs                             :: 24
            ncRNAs                            :: 2
            Pseudo Genes (total)              :: 36
            CDSs (without protein)            :: 36
            Pseudo Genes (ambiguous residues) :: 0 of 36
            Pseudo Genes (frameshifted)       :: 2 of 36
            Pseudo Genes (incomplete)         :: 32 of 36
            Pseudo Genes (internal stop)      :: 2 of 36
            ##Genome-Annotation-Data-END##
FEATURES             Location/Qualifiers
     source          1..3273
                     /organism="Micropruina sp."
                     /mol_type="genomic DNA"
                     /submitter_seqid="NODE_11985_length_3273_cov_4.376942"
                     /isolate="AMR_MDS_2195"
                     /isolation_source="Activated sludge of municipal
                     wastewater treatment plants"
                     /db_xref="taxon:2737536"
                     /environmental_sample
                     /geo_loc_name="Canada"
                     /lat_lon="49.25 N 123.1 W"
                     /collection_date="2015-06-16"
                     /metagenome_source="wastewater metagenome"
                     /note="metagenomic"
     gene            <1..560
                     /locus_tag="PLF56_11070"
     CDS             <1..560
                     /locus_tag="PLF56_11070"
                     /EC_number="4.1.1.48"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_013863812.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_function: GO:0004425 - indole-3-glycerol-phosphate
                     synthase activity [Evidence IEA];
                     GO_process: GO:0000162 - tryptophan biosynthetic process
                     [Evidence IEA]"
                     /codon_start=3
                     /transl_table=11
                     /product="indole-3-glycerol phosphate synthase TrpC"
                     /protein_id="HOQ54140.1"
                     /translation="AEYAAGGASAISVLTERRRFGGSLEDLVAVRASVDVPVLRKDFI
                     VTDYQLWEARAAGADLALLIVASLSDAELARLMALAQQLGLTALVEAHTADEVRRALD
                     AGASLLGVNNRNLQTLEVDLRQFERLASLIPSGVVKVAESGILSTADVAVVVEAGADV
                     ILVGEALVRHGDPRQAVRDFISTGS"
     gene            692..1246
                     /locus_tag="PLF56_11075"
     CDS             692..1246
                     /locus_tag="PLF56_11075"
                     /inference="COORDINATES: protein motif:HMM:TIGR02937.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_function: GO:0016987 - sigma factor activity [Evidence
                     IEA];
                     GO_process: GO:0006352 - DNA-templated transcription
                     initiation [Evidence IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="RNA polymerase sigma factor"
                     /protein_id="HOQ54141.1"
                     /translation="MRRIAGDRDRAWLEQLFDVHSRQLRAFAVRRVGLDAADDVVSEV
                     FATAWRRRSDVPEPALAWLFQTARHIVLHQVRSASRRVALHATVRNQRQPAAAPSAED
                     AARVLIEAVLDELPATEAEILRLTVWDQLTPSEIAVALSITPGATRTRLMRARQHAQE
                     IYEATLLADVADPTMSAERSASQP"
     gene            1256..2272
                     /locus_tag="PLF56_11080"
     CDS             1256..2272
                     /locus_tag="PLF56_11080"
                     /inference="COORDINATES: protein motif:HMM:NF038083.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="CU044_5270 family protein"
                     /protein_id="HOQ54142.1"
                     /translation="MSLLFHESMRDGDPAAGLASYSPDQRREILDAAVDATPPSPQRR
                     SRIRVLLAACAALVVAGLGYQSVTASVGAAQATEVLTQAAISASDPHAKQGQYWEITR
                     IGTTLYSDTQDRWFIVRTTGVDYVAVDGKRPSWFVRGGARTVRQLAGTPSDDPVTWAP
                     REAWTTNLAPEQKPAWWSEPSPSWLAALPRDTTALRERLYTDTRGHGTNPDDEAFVYV
                     ADVLRSGVVPADLRSALYSVLKTIPGTVVVDRQVTLEDGRSGVAIGRNDIFGTHNEFV
                     IAPGTGEVIGDRSFMRLTPLVGERITLSTGVQRRLVDSVPQDVQRIATRQTCKVVTGE
                     VVCD"
     gene            2406..>3273
                     /gene="trpB"
                     /locus_tag="PLF56_11085"
     CDS             2406..>3273
                     /gene="trpB"
                     /locus_tag="PLF56_11085"
                     /EC_number="4.2.1.20"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_016669811.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_function: GO:0004834 - tryptophan synthase activity
                     [Evidence IEA];
                     GO_process: GO:0000162 - tryptophan biosynthetic process
                     [Evidence IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="tryptophan synthase subunit beta"
                     /protein_id="HOQ54143.1"
                     /translation="MSLPDAAGHFEIFGGKYVPEALYAALAQLEAEFDRAMDDPAFWA
                     ELDDLRRNYSGRPTPLTEAKRFAEHCGNAIVLLKREDLNHTGSHKINNVLGQALLTKR
                     MGKTRVIAETGAGQHGVATATAAALLGLECRVYMGKVDTDRQALNVARMQLLGAEVIA
                     VESGSQTLKDAMNDAMRDWVASVDSTHYLIGTVAGPHPFPKMVREFQRVISTETRAQV
                     LERFGRLPDAVTACVGGGSNAMGMFADFVHEPTVALYGFEAGGDGVETGRHASAIHGG
                     SVGVLHGMRTYIL"
BASE COUNT          536 a         1132 c         1099 g          506 t
ORIGIN      
        1 ccgccgagta cgccgcgggg ggagcgtccg cgatctcggt gctgaccgag cggcgccgct
       61 tcggtggttc gctggaggat ctggtggccg tccgggcctc cgtcgacgtg ccggtgctgc
      121 gcaaggattt catcgtcacc gattaccagc tgtgggaggc gcgggccgcc ggagccgacc
      181 tggcgctgct gatcgtggcc tccctgagcg atgccgaact ggcgcggctg atggcgctgg
      241 cgcagcagct cgggctgacc gccctggtcg aggcgcacac cgccgacgag gtgcgccggg
      301 cgctggacgc cggggcgtcc ctgctcgggg tcaacaaccg caacctgcag accctcgagg
      361 tcgacctgcg gcagttcgaa cggctggcgt cgctgattcc gtccggggtg gtcaaggtgg
      421 ccgagtcggg cattctctcg accgcggacg tcgcggtggt cgtcgaggcg ggcgccgacg
      481 tgatcctggt cggcgaggcg ctggtccggc acggcgaccc gcggcaggcg gtgcgcgact
      541 tcatcagcac cggctcatga gggacggctc ttcgttccgg cgcgcagcga ccgaaccacc
      601 cctcgagagc cagaagggtc acgaacccga gcctttgggt attcgcaccc atcccgcgta
      661 acgcggacag cctgagcggg aactacatgg catgagacgc atcgccggtg accgggaccg
      721 cgcctggctc gagcaactat tcgacgttca cagccggcag ctgcgcgctt tcgctgtccg
      781 cagagtcggt ctggacgccg ccgacgacgt tgtctcagag gtgttcgcaa cagcctggcg
      841 tcggcgcagc gacgttcccg aaccggcact cgcctggttg ttccaaactg cgcggcacat
      901 cgtcttgcac caagtccgca gcgccagccg ccgggtggct ctgcatgcca ccgtccgaaa
      961 ccagcggcag ccggccgctg caccgtccgc cgaagacgcg gcccgcgtgt tgatcgaggc
     1021 cgtgctggac gaactgccgg ccaccgaggc cgagatcctc cggctcaccg tgtgggatca
     1081 gctcacgccc tcggagatcg ccgtcgccct gtcgatcacc ccgggagcga cccggacccg
     1141 gctgatgcgg gcgcggcaac acgcgcagga aatctacgaa gcgactcttc tcgccgatgt
     1201 cgccgacccc accatgtccg ctgagcgcag cgcgtcccag ccctgaggag tgaccatgtc
     1261 cctgttgttc cacgagtcca tgcgagacgg cgacccggcg gcgggcttgg cgtcgtattc
     1321 acccgaccaa cgccgcgaga tcctcgacgc tgcggttgac gcaacaccgc cttccccaca
     1381 gcggcgctca cgcatccgcg tcctcctcgc agcgtgtgcc gcgctggtcg tcgccggcct
     1441 gggctaccag tcggtaaccg cgagtgtcgg cgccgcgcaa gcgacggagg tgctcactca
     1501 agccgccatc agcgcatcgg acccgcacgc caagcagggg cagtactggg agatcacccg
     1561 gatcggcacc accctctata gcgacacgca ggaccgctgg ttcatcgtcc gcaccactgg
     1621 cgtcgactat gtggcggtcg atggaaagcg gccgagttgg ttcgtgcgag gaggcgctcg
     1681 aactgttcga cagcttgccg gcaccccgtc ggacgatccg gtcacctggg cgccccgcga
     1741 agcatggacg acgaacctgg ccccggagca gaagcccgcc tggtggagtg aaccatcacc
     1801 gtcatggctt gccgcattgc ctcgcgacac gaccgcgctt cgcgaacggc tatacaccga
     1861 cacccgtggc cacgggacca acccagacga cgaagcgttc gtgtacgtgg ccgacgtgct
     1921 gcgctccggc gtcgtgcctg ctgatctgcg ctcggcgctc tacagcgtgc tcaagaccat
     1981 tcccggcacc gtcgtcgtcg atcggcaggt cacactggag gacggccgca gcggcgtggc
     2041 gatcggccgc aacgacatct tcggcaccca caacgagttc gtgatcgctc caggtaccgg
     2101 cgaagtgatc ggcgaccgca gttttatgcg gttgactccg ctcgtgggcg agcgcatcac
     2161 gctcagtacc ggagtccagc ggcggctggt cgactcggtg ccgcaggacg tgcagcgcat
     2221 cgccacccgg cagacctgca aggtagtcac gggcgaggtc gtctgcgact gaacctccag
     2281 cgacctcgat cggtgctgcg caactggcta ccgtgtcgga tgtaccagcg gtgggcgcag
     2341 ccactgggag atgctccggt tgcgtagagt ggagcgtccg cacccagacg aaccgaggat
     2401 tcaccgtgtc gttacccgat gccgcagggc atttcgagat cttcgggggc aagtacgttc
     2461 ccgaagccct gtacgccgct ctggcccaac ttgaggcaga gttcgatcgg gccatggacg
     2521 accccgcctt ctgggcggag ctggacgatc ttcggcgcaa ctattccggc cgccccaccc
     2581 cgctgaccga ggccaaacgg ttcgccgagc actgcggcaa cgcgatcgtg ctgctcaagc
     2641 gtgaggacct caaccacacc ggcagccaca agatcaacaa cgtgctgggc caggcgctgc
     2701 tgaccaagcg gatgggcaag acccgggtga tcgccgaaac cggcgcgggc cagcacgggg
     2761 tcgccaccgc cacggctgcg gcgctgctcg gcctggagtg ccgggtctac atgggcaagg
     2821 tcgacaccga ccgccaagcg ctcaacgtgg cccgcatgca gttgctgggc gccgaggtga
     2881 tcgccgtcga atccggtagc cagacgctga aagatgcgat gaacgacgcg atgcgcgact
     2941 gggtcgccag cgtcgattcc acccactacc tgatcggcac cgtcgctggg ccgcacccgt
     3001 tcccgaagat ggtccgcgag ttccagcggg tgatctcgac cgagacccgg gcccaggtgc
     3061 tcgaacggtt cggacgcctg cccgacgccg tcaccgcctg cgtgggtggc ggatccaacg
     3121 cgatgggaat gttcgccgac ttcgtgcacg agccgaccgt cgcgctgtac ggcttcgagg
     3181 ccggcggcga cggggtggag accggacgac acgcgtccgc gatccacggt ggctcggtgg
     3241 gcgtgctgca cggcatgcgc acctacattc tgc
//
LOCUS       DAOSUG010000363         3264 bp    DNA     linear   ENV 10-JUN-2024
DEFINITION  MAG TPA_asm: Micropruina sp. isolate AMR_MDS_2195
            NODE_12011_length_3264_cov_3.336865, whole genome shotgun sequence.
ACCESSION   DAOSUG010000363 DAOSUG010000000
VERSION     DAOSUG010000363.1
DBLINK      BioProject: PRJNA925477
            BioSample: SAMN32864262
            Sequence Read Archive: SRR4236673
KEYWORDS    WGS; ENV; Metagenome Assembled Genome; MAG; Third Party Data; TPA;
            TPA:assembly.
SOURCE      Micropruina sp. (wastewater metagenome)
  ORGANISM  Micropruina sp.
            Bacteria; Bacillati; Actinomycetota; Actinomycetes;
            Propionibacteriales; Nocardioidaceae; Micropruina.
REFERENCE   1  (bases 1 to 3264)
  AUTHORS   Abdulkadir,N., Saraiva,J.P., Zhang,J., Stolte,S., Gillor,O.,
            Harms,H. and Rocha,U.
  TITLE     Genome-centric analyses of 165 metagenomes show that mobile genetic
            elements are crucial for the transmission of antimicrobial
            resistance genes to pathogens in activated sludge and wastewater
  JOURNAL   Microbiol Spectr 12 (3), e0291823 (2024)
   PUBMED   38289113
REFERENCE   2  (bases 1 to 3264)
  AUTHORS   Abdulkadir,N.
  TITLE     Direct Submission
  JOURNAL   Submitted (24-MAR-2023) Environmental Microbiology, Helmholtz
            Center for Environmental Research -UFZ, Permoserstr 15, Leipzig
            04318, Germany
COMMENT     The annotation was added by the NCBI Prokaryotic Genome Annotation
            Pipeline (PGAP). Information about PGAP can be found here:
            https://www.ncbi.nlm.nih.gov/genome/annotation_prok/
            
            ##Genome-Assembly-Data-START##
            Assembly Method        :: SPAdes v. 3.11.1
            Genome Representation  :: Full
            Expected Final Version :: Yes
            Genome Coverage        :: Not Applicable
            Sequencing Technology  :: Illumina MiSeq
            ##Genome-Assembly-Data-END##
            
            ##Genome-Annotation-Data-START##
            Annotation Provider               :: NCBI
            Annotation Date                   :: 05/04/2023 04:57:10
            Annotation Pipeline               :: NCBI Prokaryotic Genome
                                                 Annotation Pipeline (PGAP)
            Annotation Method                 :: Best-placed reference protein
                                                 set; GeneMarkS-2+
            Annotation Software revision      :: 6.5
            Features Annotated                :: Gene; CDS; rRNA; tRNA; ncRNA
            Genes (total)                     :: 2,776
            CDSs (total)                      :: 2,750
            Genes (coding)                    :: 2,714
            CDSs (with protein)               :: 2,714
            Genes (RNA)                       :: 26
            tRNAs                             :: 24
            ncRNAs                            :: 2
            Pseudo Genes (total)              :: 36
            CDSs (without protein)            :: 36
            Pseudo Genes (ambiguous residues) :: 0 of 36
            Pseudo Genes (frameshifted)       :: 2 of 36
            Pseudo Genes (incomplete)         :: 32 of 36
            Pseudo Genes (internal stop)      :: 2 of 36
            ##Genome-Annotation-Data-END##
FEATURES             Location/Qualifiers
     source          1..3264
                     /organism="Micropruina sp."
                     /mol_type="genomic DNA"
                     /submitter_seqid="NODE_12011_length_3264_cov_3.336865"
                     /isolate="AMR_MDS_2195"
                     /isolation_source="Activated sludge of municipal
                     wastewater treatment plants"
                     /db_xref="taxon:2737536"
                     /environmental_sample
                     /geo_loc_name="Canada"
                     /lat_lon="49.25 N 123.1 W"
                     /collection_date="2015-06-16"
                     /metagenome_source="wastewater metagenome"
                     /note="metagenomic"
     gene            complement(1..133)
                     /locus_tag="PLF56_11090"
                     /pseudo
     CDS             complement(1..133)
                     /locus_tag="PLF56_11090"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_004810606.1"
                     /note="incomplete; partial in the middle of a contig;
                     missing N-terminus and C-terminus; Derived by automated
                     computational analysis using gene prediction method:
                     Protein Homology."
                     /pseudo
                     /codon_start=1
                     /transl_table=11
                     /product="DNA-binding response regulator"
     gene            complement(375..1043)
                     /locus_tag="PLF56_11095"
     CDS             complement(375..1043)
                     /locus_tag="PLF56_11095"
                     /inference="COORDINATES: protein motif:HMM:NF012655.2"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_function: GO:0003677 - DNA binding [Evidence IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="helix-turn-helix domain-containing protein"
                     /protein_id="HOQ54144.1"
                     /translation="MTKDESVDAGRPSRQKQVGDALRTDTRARLLQAAEREFAARGYT
                     GTTVTRLAAAAGVSVQTLYLAWGNKRGLLRGYLENALAGRATTPEDAAAGLAGLPPKE
                     RLAGLARLVATIAERAALGWTLYRDAAVVDPEIAADWDELQLLRHRTFTRIVGDIPTT
                     ALRDGLSRAAAIDTAWTIASPETFDLLTRRLDYSVDQFRDWLEHTLIAALLEPTAQPS
                     RPSG"
     gene            1233..2867
                     /locus_tag="PLF56_11100"
     CDS             1233..2867
                     /locus_tag="PLF56_11100"
                     /inference="COORDINATES: protein motif:HMM:TIGR00976.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_function: GO:0016787 - hydrolase activity [Evidence
                     IEA];
                     GO_process: GO:0008152 - metabolic process [Evidence IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="CocE/NonD family hydrolase"
                     /protein_id="HOQ54145.1"
                     /translation="MPADVLKQQDVAVRMRDGVTLRLNLYRPAGPELALPVLLSAHPY
                     GKDAVPVRRGRRWRINPQFRIMNQPAPVRISDQTSWEAPDPVWWVGQGYAVINLDVRG
                     GGRSEGRGALFSDQEADDIAQVIAWAAAQPWSNGRVGMLGVSYLAISQYKVAARNPPA
                     LKAICPWEGFTDPYRDFFSPGGVVEDGFARIWLFLSSRVARLSTDLEVERQRHPLRDA
                     WWESLTPDLAAITVPILECTSFSDANLHSGGSMRAFQQVASVHRHAYAHRGPKWATFY
                     GEDARQHQLAFFDRYLRDRPAPEPARLRLEIRDRRDHVVEVRDEQEGPLGRTQWRWLH
                     LGADGVLSDTETAAGTVTFGLRSHAAAFDYRFAQDTELTGPMTLRLRVATLGARDPRL
                     FVGVEKWASGAPVPFEGSYGYGRDHVAQGRLRLALRELDPQRSAPHQPVHAFRRVLPV
                     RDGEEAEVLIPLSPSATIFHAGDRLRLLIAGRYLQPRNPLFGHFPTHYRPSGAGRATI
                     AWAPGAPSALEIPVIPRAPSELNISDRAAEANHPDA"
     gene            2908..>3264
                     /locus_tag="PLF56_11105"
     CDS             2908..>3264
                     /locus_tag="PLF56_11105"
                     /inference="COORDINATES: protein motif:HMM:NF012632.2"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="hypothetical protein"
                     /protein_id="HOQ54146.1"
                     /translation="MTPVRQDPAPSETAATPRGVPAYAWGYNVRAGLGLGHTARVLTP
                     TPLRLPKGTVDVQGGVNFSIALTRGGKLYAWGGNQYGQLGDGTTSVRWVPTRVALPKG
                     TTICSIAVGTDHVVATT"
BASE COUNT          472 a         1153 c         1150 g          489 t
ORIGIN      
        1 ccagccgcac cggagtgccg ttgaccgaga cctggtgccg ctcgatgtcc atacggacac
       61 cgtgcgcttc caccacatcc ggcatcagct cggtctcctg cccacggcgc agcaccgccc
      121 ggatccgggc gatcgtggac agcacctcgc cgagctcgct ggcgccgtga acggcccggc
      181 cgagcacgcc ccgggtggcg tagtcgagat cgctgtcgcg gaagaacccg ccgtcgatca
      241 cgtcgtgtgc gtctctgccg agcctcatgg cgattcccct gtcgatgccg gtgcgatcca
      301 cccctgccgg cggatccgtt cgagtgccat cccaccgtgg cgaggcgtgc gagggactga
      361 ccgaaatgac cagttcagcc ggagggccgg ctcggttgtg cggtgggctc gagcagggcg
      421 gcgatcaggg tgtgctccag ccagtcgcgg aactgatcca cgctgtagtc gaggcgccgg
      481 gtgagcagat cgaacgtctc ggggctcgcg atcgtccacg cggtgtcgat cgcggcggcc
      541 cggctgaggc cgtcgcgcag cgcggtcgtg gggatgtcgc ccacgatccg ggtgaaggtc
      601 cggtgacgca gaagctgcag ttcgtcccag tcggccgcga tctctgggtc gaccaccgct
      661 gcgtcccggt agagcgtcca gccgagagcg gcacgctcgg cgatcgtggc caccagccgt
      721 gcgagcccgg ccagccgttc ctttggcggc aacccggcca gtccggcggc ggcgtcctcg
      781 ggggttgtgg cccggcccgc cagggcgttc tcgaggtagc cacgcagcag tccgcgctta
      841 ttgccccagg cgaggtagag cgtctgaacg gacaccccgg cggcggcggc cagccgcgtg
      901 accgtggtgc cggtgtagcc ccgcgctgcg aactcccgct cggcggcctg caggagtcgg
      961 gcccgggtgt cggtgcgcag ggcgtcgccg acctgcttct ggcgactggg gcggcccgcg
     1021 tcgacggatt cgtccttggt catggacagc atcgtagttc atgttttaga gtgccgttct
     1081 atagcatttg aatggcattc aaaagcaact gcgaaggagg tgtggacgtg actcgtatcg
     1141 ggcgggacaa gccctggcgg cgaccggggc gggtggcgta cgcagcacac cgctggtccg
     1201 cgttcaggcg gcctgcggtg tccgtccatc cgatgccggc cgacgtcctc aagcagcagg
     1261 acgtggccgt gcgaatgcgc gatggggtca ccctgcggct gaatctctac cggccggccg
     1321 ggccggaact cgccctgccc gtgctgctct cggcccaccc gtacggcaag gacgccgtac
     1381 cggtcaggag gggccgccgg tggcggatca acccgcagtt ccggatcatg aaccagccgg
     1441 ccccggtgcg gatctcggat cagaccagtt gggaggcgcc cgacccggtc tggtgggtgg
     1501 ggcagggcta cgcggtgatc aacctggacg ttcgcggcgg cggacgctcc gagggtcgcg
     1561 gtgcactgtt ctctgaccag gaagccgacg acatcgccca ggtgatcgcc tgggcggcgg
     1621 cgcagccctg gtcgaacggc cgcgtgggga tgctgggcgt gtcctacctg gccatctccc
     1681 agtacaaggt cgccgcgcgc aacccgccgg cgctgaaggc gatctgcccg tgggagggat
     1741 tcaccgaccc ctaccgcgac ttcttcagcc ccggcggcgt cgtcgaggac ggtttcgccc
     1801 ggatctggct gttcctcagt tcgcgggtgg ccaggttgag caccgacctc gaggtcgagc
     1861 gtcagcggca tccgttgcgg gacgcctggt gggagtcgct cacccccgac ctggcggcga
     1921 tcaccgtccc gattctcgag tgcaccagct tctccgacgc caatctgcac agcgggggat
     1981 cgatgcgagc cttccagcag gtggcctccg tgcatcgcca cgcgtacgcg caccgcgggc
     2041 ccaagtgggc gaccttctac ggcgaggacg cccgacagca ccagctcgcc ttcttcgacc
     2101 ggtacctccg cgaccggccg gcgcccgagc cggcccgcct gcggctcgag atcagggatc
     2161 ggcgcgatca cgtggtggag gtgcgggacg agcaggaggg gccgctcggc cgcacgcagt
     2221 ggcggtggct ccacctcggc gcggacggcg tgctcagcga caccgagacc gcggccggca
     2281 cggtgacgtt cggcctcaga agtcacgccg ccgcgttcga ctaccgcttc gctcaggaca
     2341 ccgaactgac cggtccgatg accctgcggc tccgggtggc gaccctgggg gcgcgtgatc
     2401 cccgactgtt cgtcggggtg gagaagtggg cgtccggggc gcccgtcccc ttcgagggct
     2461 cgtacgggta cgggcgcgat cacgtcgcgc agggccggct gcggctcgcc ctgcgcgaac
     2521 tcgacccgca acgcagcgca ccccaccagc ccgtccacgc gttccgcagg gtactaccgg
     2581 tgcgcgacgg cgaggaggcc gaggtgctga tccccttgag cccctcggcg accatcttcc
     2641 acgccggcga ccggcttcgg ctgctgatcg ccggccgcta cctgcagccg cgcaacccgc
     2701 tgttcggaca cttcccgacg cactatcggc caagcggagc cggccgggcc acgatcgcct
     2761 gggcacccgg cgcaccctcg gccctggaga tccccgtcat cccgcgcgcg ccgagcgaac
     2821 tgaacatttc ggacagggcc gccgaggcga accacccgga cgcttgatgc acacctctca
     2881 tcaccaccag gaggacccgt ggagaccatg acacccgttc gccaggatcc cgcaccatcg
     2941 gagaccgcag ccaccccgag gggcgtgccg gcctacgcct ggggctacaa cgtgcgcgcc
     3001 ggactcggcc tcggccacac tgcgcgggtc ctgacgccca ctcccctccg gctgccgaag
     3061 gggaccgtcg acgtgcaggg aggggtgaac ttcagcatcg ccctgacccg gggcgggaag
     3121 ctctatgcct ggggcggcaa ccagtacggc cagttgggtg acggcacgac gtccgtgcgc
     3181 tgggtcccga cgcgggtcgc gctgccgaag gggaccacga tctgctcgat cgcggtcggc
     3241 accgaccatg tggtggcgac cact
//
LOCUS       DAOSUG010000364         3263 bp    DNA     linear   ENV 10-JUN-2024
DEFINITION  MAG TPA_asm: Micropruina sp. isolate AMR_MDS_2195
            NODE_12018_length_3263_cov_5.865960, whole genome shotgun sequence.
ACCESSION   DAOSUG010000364 DAOSUG010000000
VERSION     DAOSUG010000364.1
DBLINK      BioProject: PRJNA925477
            BioSample: SAMN32864262
            Sequence Read Archive: SRR4236673
KEYWORDS    WGS; ENV; Metagenome Assembled Genome; MAG; Third Party Data; TPA;
            TPA:assembly.
SOURCE      Micropruina sp. (wastewater metagenome)
  ORGANISM  Micropruina sp.
            Bacteria; Bacillati; Actinomycetota; Actinomycetes;
            Propionibacteriales; Nocardioidaceae; Micropruina.
REFERENCE   1  (bases 1 to 3263)
  AUTHORS   Abdulkadir,N., Saraiva,J.P., Zhang,J., Stolte,S., Gillor,O.,
            Harms,H. and Rocha,U.
  TITLE     Genome-centric analyses of 165 metagenomes show that mobile genetic
            elements are crucial for the transmission of antimicrobial
            resistance genes to pathogens in activated sludge and wastewater
  JOURNAL   Microbiol Spectr 12 (3), e0291823 (2024)
   PUBMED   38289113
REFERENCE   2  (bases 1 to 3263)
  AUTHORS   Abdulkadir,N.
  TITLE     Direct Submission
  JOURNAL   Submitted (24-MAR-2023) Environmental Microbiology, Helmholtz
            Center for Environmental Research -UFZ, Permoserstr 15, Leipzig
            04318, Germany
COMMENT     The annotation was added by the NCBI Prokaryotic Genome Annotation
            Pipeline (PGAP). Information about PGAP can be found here:
            https://www.ncbi.nlm.nih.gov/genome/annotation_prok/
            
            ##Genome-Assembly-Data-START##
            Assembly Method        :: SPAdes v. 3.11.1
            Genome Representation  :: Full
            Expected Final Version :: Yes
            Genome Coverage        :: Not Applicable
            Sequencing Technology  :: Illumina MiSeq
            ##Genome-Assembly-Data-END##
            
            ##Genome-Annotation-Data-START##
            Annotation Provider               :: NCBI
            Annotation Date                   :: 05/04/2023 04:57:10
            Annotation Pipeline               :: NCBI Prokaryotic Genome
                                                 Annotation Pipeline (PGAP)
            Annotation Method                 :: Best-placed reference protein
                                                 set; GeneMarkS-2+
            Annotation Software revision      :: 6.5
            Features Annotated                :: Gene; CDS; rRNA; tRNA; ncRNA
            Genes (total)                     :: 2,776
            CDSs (total)                      :: 2,750
            Genes (coding)                    :: 2,714
            CDSs (with protein)               :: 2,714
            Genes (RNA)                       :: 26
            tRNAs                             :: 24
            ncRNAs                            :: 2
            Pseudo Genes (total)              :: 36
            CDSs (without protein)            :: 36
            Pseudo Genes (ambiguous residues) :: 0 of 36
            Pseudo Genes (frameshifted)       :: 2 of 36
            Pseudo Genes (incomplete)         :: 32 of 36
            Pseudo Genes (internal stop)      :: 2 of 36
            ##Genome-Annotation-Data-END##
FEATURES             Location/Qualifiers
     source          1..3263
                     /organism="Micropruina sp."
                     /mol_type="genomic DNA"
                     /submitter_seqid="NODE_12018_length_3263_cov_5.865960"
                     /isolate="AMR_MDS_2195"
                     /isolation_source="Activated sludge of municipal
                     wastewater treatment plants"
                     /db_xref="taxon:2737536"
                     /environmental_sample
                     /geo_loc_name="Canada"
                     /lat_lon="49.25 N 123.1 W"
                     /collection_date="2015-06-16"
                     /metagenome_source="wastewater metagenome"
                     /note="metagenomic"
     gene            complement(178..468)
                     /locus_tag="PLF56_11110"
     CDS             complement(178..468)
                     /locus_tag="PLF56_11110"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_002512897.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="cell division protein CrgA"
                     /protein_id="HOQ54147.1"
                     /translation="MPESRTRKQAAEKKKQSSADAAVEARKDRQRRVAAPGSRRWVPP
                     TFITVGLFGVLWLVVYYIAGHLIPFMGELGGWNVLIGMGCMAAAFGIATLWK"
     gene            551..1312
                     /locus_tag="PLF56_11115"
     CDS             551..1312
                     /locus_tag="PLF56_11115"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_013866119.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="DUF881 domain-containing protein"
                     /protein_id="HOQ54148.1"
                     /translation="MRRPLARARLSLRRASKRSGEVQLVRRLLSIAVCLFAGFMIATS
                     AINAGGTDLRPNRNTDLAGLVGSESKRNQELADKVAALRKDVDSLAKGDGGGVSERAM
                     AEAAALAGQTPVRGPAVAVVLDDAPSGVHPAGVSAELLVVHQQDIQAVVNTLWSGGAE
                     AMTIQGQRVTSRTGVKCVGNSVVLHGVPYAPPYEIVAIGDQGRLEQALASSAALQIYR
                     QYVDAYGLGYSQRREAEVTMPAYRGSLDIEARRIG"
     gene            1342..1974
                     /locus_tag="PLF56_11120"
     CDS             1342..1974
                     /locus_tag="PLF56_11120"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_012917745.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="aminodeoxychorismate/anthranilate synthase
                     component II"
                     /protein_id="HOQ54149.1"
                     /translation="MARVLVIDNYDSFVYNLVQYLAQLGAEVDVWRNDDERFAADDWA
                     EGYDGVLISPGPGTPESAGVCVDVVKSHGGRVPIFGVCLGLQSIAVAYGGVVNRAPEL
                     LHGKTSAVVHDGTGVFAGLPSPLTTTRYHSLALDPATVPDELEVTARTESGVIMAVRH
                     RSLPVESVQFHPESVLTEGGYQMLANWLAACGDAGAPARAVGLTPLMALR"
     gene            complement(2005..>3263)
                     /locus_tag="PLF56_11125"
     CDS             complement(2005..>3263)
                     /locus_tag="PLF56_11125"
                     /inference="COORDINATES: protein motif:HMM:NF012298.2"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=3
                     /transl_table=11
                     /product="PASTA domain-containing protein"
                     /protein_id="HOQ54150.1"
                     /translation="AAVIGTAQYLSPEQARGETVDSRSDIYSAGCLLYELLVGQPPFR
                     GDSPVSVAYQHVRETPVPPSQLDPLVTSSMDAIVLKALAKDPADRYQTASDMRDDINR
                     LLAGHAVKATAPVAVPVAAGAMDATRVLPPSGSPVTPDSTDDTDDGTVPQRAVEQPRR
                     KGAWIWGIVAFLLVIGLGYGAYLFFNPDARTETVAVPNVFDLRQDAAEQAIKEAKLVV
                     VVQSVTGPDDASLNRVTNQNPVGGVKVAVGSTVTIEVNAGPKKATIPSDLVGKSLSDA
                     KKSLADLGFTKVTGVVDESSTAKANSVLSVQPTAGANVALDTPITLTYAPGTGTVPNF
                     YGQTRSAAQAQADREGLSVKFTEAETTELAPGRVFQQNPAAGEQIKRSATITLTLAKA
                     PPTPSPSPSPTPTDTETATPEPSETP"
BASE COUNT          467 a         1104 c         1187 g          505 t
ORIGIN      
        1 cgcgcaggcg ctcagcgaca ccgacagggc gatgccgagc gctgcggtca gggctgcccg
       61 cacccgggct gtggggccga tgcgtcgagg tcgtgctgag gtcacgtaga acaccgtagg
      121 gggcgcgtcc gacaggttcg tgcggctggg ccgagtgtcg gccagtcggt gccggcgcta
      181 cttccagagc gtggcgatac cgaaggcggc tgccatgcag cccatgccga tcaacacgtt
      241 ccagccaccc agctcgccca tgaacggaat caggtggccg gcgatgtagt agaccaccag
      301 ccacaacacg ccgaacaggc cgacggtgat gaacgtgggc ggcacccacc ggcggctgcc
      361 gggggcggcg acgcggcgct ggcgatcctt gcgcgcctcg accgcggcgt cggcagagct
      421 ctgcttcttc ttctccgcgg cctgcttgcg cgttctggac tccggcatgg ggtccacctt
      481 accgtccccg gcgctcgagg tctccgccgc agcgtgggtc ctagactcgg cgcatggacg
      541 agtgcagcgc atgaggcgcc cgttggcgcg ggcccggctg tcgctgcgcc gggcgtccaa
      601 gcgttccggc gaggtgcagc tcgtgcgccg gctgctcagc atcgccgtgt gcctgttcgc
      661 cggcttcatg atcgccacca gcgcgatcaa cgccggcggc accgacctgc gccccaaccg
      721 caacaccgac ctggccgggc tggtcggctc cgaatccaag cgcaaccagg aactcgccga
      781 caaggtcgcc gccctgcgca aagatgtgga ctcgctcgcc aagggcgatg gcggcggcgt
      841 gtccgagcgg gcgatggccg aagcggccgc gctggccggc cagaccccgg tgcgcgggcc
      901 ggcggtcgcg gtcgtgctgg acgacgcacc gtccggtgtg catccggccg gggtcagcgc
      961 tgagcttttg gtggtgcacc agcaggacat tcaggccgtg gtgaacacgc tgtggagcgg
     1021 tggggccgag gcgatgacca ttcagggcca gcgggtcacc tcgcgcaccg gcgtcaaatg
     1081 cgtcggcaac tcggtggtgc tgcacggggt gccgtacgca ccgccgtacg agatcgttgc
     1141 gatcggtgac cagggcaggc tcgaacaggc actggcgagt tctgccgcac tgcagatcta
     1201 tcggcagtac gtcgacgcgt acggcctcgg ctacagccag cggcgcgagg ccgaggtcac
     1261 catgccggcc tatcgtggca gcctcgacat cgaagccaga cgcatcggct gagcacgccc
     1321 gcgatgaggg acaatgcagc catggcccgc gttctggtga tcgacaacta cgactcgttc
     1381 gtctacaacc tcgtgcagta cctggcgcag ctgggtgccg aggtcgacgt gtggcgcaac
     1441 gacgacgagc ggttcgccgc cgacgactgg gcagagggct acgacggggt gctgatctcg
     1501 cccggcccgg gcacgcccga atcggcgggc gtgtgcgtcg atgtggtcaa gtcccacggc
     1561 ggacgggtgc cgatcttcgg cgtctgcctc ggcctgcagt cgatcgcggt ggcctacggc
     1621 ggcgtggtga accgggcgcc ggagttgctg cacggcaaga cctcggccgt ggtgcacgac
     1681 ggcacgggcg tgttcgccgg cctgccgagc ccgttgacca ccacccggta ccactcgctg
     1741 gcgctcgacc cggccacggt gccggacgag ctcgaggtca cggcccgcac cgaatccggg
     1801 gtgatcatgg cggtgcggca ccgcagcctg ccggtcgagt ccgtgcagtt ccaccccgag
     1861 tcggtgctca ccgagggcgg ttaccagatg ttggccaact ggctggccgc ctgcggtgac
     1921 gccggggcgc ccgcgcgggc ggtgggcctc acgcccctga tggccctgcg ctgagaaggc
     1981 gccgatggag cgaccaacga cgtcctacgg ggtctcggac ggctccggcg tggccgtttc
     2041 ggtgtcggtc ggtgtcggcg acggcgaggg gctcggagtc ggcggcgcct tcgcgagggt
     2101 cagcgtgatc gtcgcactgc gcttgatctg ctcgccggcc gccgggttct gctgaaacac
     2161 ccgtcccggt gcgagttcgg tggtctcggc ctcggtgaac ttgacgctga gcccctcgcg
     2221 atcggcctgg gcctgcgcgg ccgagcgggt ctgcccgtag aagttgggca cagtgccggt
     2281 tccgggggcg taggtgagcg tgatcggggt gtccagggcc acgttcgcgc ccgcggtggg
     2341 ctgcacgctg agcaccgagt tggccttcgc cgtggacgac tcgtcgacca cgccggtcac
     2401 cttggtgaac ccgaggtcgg caagcgactt cttggcgtcg ctgagcgact tgcccacgag
     2461 atcggacgga atggtcgcct tcttcggccc cgcgttcacc tcgatcgtga cggtggagcc
     2521 gaccgcgacc ttcaccccgc cgaccggatt ctgattggtc acccggttca ggctcgcgtc
     2581 gtccgggccg gtcacggact gcaccaccac gacgagcttg gcctctttga tggcctgctc
     2641 ggcggcgtcc tggcgcaggt cgaacacgtt cggcaccgcc acggtctcgg tgcgggcatc
     2701 ggggttgaag aacaggtagg cgccgtagcc gaggccgatc accagcagga acgccacgat
     2761 gccccagatc caggccccct tgcgccgcgg ctgctcgacg gcacgctggg gcacggtgcc
     2821 gtcgtcggtg tcgtccgtcg agtcgggggt gaccggtgag ccggacgggg gcagcacgcg
     2881 ggtggcgtcc atggcgccgg cggccaccgg caccgcaacc ggtgcggtcg ccttcaccgc
     2941 atgaccggcc agcagccggt tgatgtcgtc gcgcatgtcg gacgccgtct ggtaccggtc
     3001 ggcgggatcc ttggccagcg ccttgagcac gatcgcgtcc atgctcgagg tgaccagcgg
     3061 gtcgagctga ctgggcggta ccggcgtttc acgcacatgc tggtaggcga ccgacaccgg
     3121 cgaatccccc cggaacggcg gctgcccgac cagcagttcg tacagcagac aaccggccga
     3181 atagatgtcc gagcgcgagt cgacggtctc accgcgggcc tgctcgggcg acaagtactg
     3241 cgcggtgccg atcacggccg cgg
//
LOCUS       DAOSUG010000365         3244 bp    DNA     linear   ENV 10-JUN-2024
DEFINITION  MAG TPA_asm: Micropruina sp. isolate AMR_MDS_2195
            NODE_12114_length_3244_cov_4.778928, whole genome shotgun sequence.
ACCESSION   DAOSUG010000365 DAOSUG010000000
VERSION     DAOSUG010000365.1
DBLINK      BioProject: PRJNA925477
            BioSample: SAMN32864262
            Sequence Read Archive: SRR4236673
KEYWORDS    WGS; ENV; Metagenome Assembled Genome; MAG; Third Party Data; TPA;
            TPA:assembly.
SOURCE      Micropruina sp. (wastewater metagenome)
  ORGANISM  Micropruina sp.
            Bacteria; Bacillati; Actinomycetota; Actinomycetes;
            Propionibacteriales; Nocardioidaceae; Micropruina.
REFERENCE   1  (bases 1 to 3244)
  AUTHORS   Abdulkadir,N., Saraiva,J.P., Zhang,J., Stolte,S., Gillor,O.,
            Harms,H. and Rocha,U.
  TITLE     Genome-centric analyses of 165 metagenomes show that mobile genetic
            elements are crucial for the transmission of antimicrobial
            resistance genes to pathogens in activated sludge and wastewater
  JOURNAL   Microbiol Spectr 12 (3), e0291823 (2024)
   PUBMED   38289113
REFERENCE   2  (bases 1 to 3244)
  AUTHORS   Abdulkadir,N.
  TITLE     Direct Submission
  JOURNAL   Submitted (24-MAR-2023) Environmental Microbiology, Helmholtz
            Center for Environmental Research -UFZ, Permoserstr 15, Leipzig
            04318, Germany
COMMENT     The annotation was added by the NCBI Prokaryotic Genome Annotation
            Pipeline (PGAP). Information about PGAP can be found here:
            https://www.ncbi.nlm.nih.gov/genome/annotation_prok/
            
            ##Genome-Assembly-Data-START##
            Assembly Method        :: SPAdes v. 3.11.1
            Genome Representation  :: Full
            Expected Final Version :: Yes
            Genome Coverage        :: Not Applicable
            Sequencing Technology  :: Illumina MiSeq
            ##Genome-Assembly-Data-END##
            
            ##Genome-Annotation-Data-START##
            Annotation Provider               :: NCBI
            Annotation Date                   :: 05/04/2023 04:57:10
            Annotation Pipeline               :: NCBI Prokaryotic Genome
                                                 Annotation Pipeline (PGAP)
            Annotation Method                 :: Best-placed reference protein
                                                 set; GeneMarkS-2+
            Annotation Software revision      :: 6.5
            Features Annotated                :: Gene; CDS; rRNA; tRNA; ncRNA
            Genes (total)                     :: 2,776
            CDSs (total)                      :: 2,750
            Genes (coding)                    :: 2,714
            CDSs (with protein)               :: 2,714
            Genes (RNA)                       :: 26
            tRNAs                             :: 24
            ncRNAs                            :: 2
            Pseudo Genes (total)              :: 36
            CDSs (without protein)            :: 36
            Pseudo Genes (ambiguous residues) :: 0 of 36
            Pseudo Genes (frameshifted)       :: 2 of 36
            Pseudo Genes (incomplete)         :: 32 of 36
            Pseudo Genes (internal stop)      :: 2 of 36
            ##Genome-Annotation-Data-END##
FEATURES             Location/Qualifiers
     source          1..3244
                     /organism="Micropruina sp."
                     /mol_type="genomic DNA"
                     /submitter_seqid="NODE_12114_length_3244_cov_4.778928"
                     /isolate="AMR_MDS_2195"
                     /isolation_source="Activated sludge of municipal
                     wastewater treatment plants"
                     /db_xref="taxon:2737536"
                     /environmental_sample
                     /geo_loc_name="Canada"
                     /lat_lon="49.25 N 123.1 W"
                     /collection_date="2015-06-16"
                     /metagenome_source="wastewater metagenome"
                     /note="metagenomic"
     gene            <1..265
                     /locus_tag="PLF56_11130"
     CDS             <1..265
                     /locus_tag="PLF56_11130"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_020575695.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_function: GO:0008236 - serine-type peptidase activity
                     [Evidence IEA];
                     GO_process: GO:0006508 - proteolysis [Evidence IEA]"
                     /codon_start=2
                     /transl_table=11
                     /product="prolyl oligopeptidase family serine peptidase"
                     /protein_id="HOQ54151.1"
                     /translation="TPMLVVHGDKDYRVPIGEGLRLWWELLSRSQLALDEAGRTPHRF
                     LYFPDENHWVLSPQHAAIWYATVSAFLSQHVLDEPSDYPELLG"
     gene            630..1946
                     /locus_tag="PLF56_11135"
     CDS             630..1946
                     /locus_tag="PLF56_11135"
                     /inference="COORDINATES: protein motif:HMM:NF018448.2"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="threonine/serine exporter family protein"
                     /protein_id="HOQ54152.1"
                     /translation="MDGQEPPRSISEHEYEDQLEPTHLIRRSHVVLRAGTMMLGAGTS
                     ALRVREVMAAVARTVGVQQIDAQVTFTQVVVTIGRRGIFRTQVGETRPSVNADRIALL
                     HQFSHDLPERERAIDVEARLDAIEDSPRLYPAWMLVILVALACASVAVLSNGGWREVL
                     AVLPASAVAYGLHRLLTRLEFNLLAVVLTSAAVASALYVAGADLITLAVGSPSDRFAA
                     GFICASIFLIPGFPLVTAGLDLTRLDLDAGIPRLSYAAMVVLAIAIGVWLVASITGVS
                     PVPVGPIEGDPALVWAALIIASFFAVFGWATMFNVPLGTAVASGVVAIVGNVPRLLLL
                     ENGIKPHVATFVGCVIMGLGCAVVAYWFHMTKIIMTVPTLLVSIPGSSALRTLIYFDQ
                     ADITAAIESGVSTVLVVVAMVAGLSGARMLTDPEWTFTRPATGRTI"
     gene            complement(2021..>3244)
                     /locus_tag="PLF56_11140"
     CDS             complement(2021..>3244)
                     /locus_tag="PLF56_11140"
                     /inference="COORDINATES: ab initio
                     prediction:GeneMarkS-2+"
                     /note="Derived by automated computational analysis using
                     gene prediction method: GeneMarkS-2+."
                     /codon_start=1
                     /transl_table=11
                     /product="hypothetical protein"
                     /protein_id="HOQ54153.1"
                     /translation="LVRDPVDDRWCSVAAPELRVRPEPAAVVWVWAAGAEGVLCVAPR
                     HRAGPVVGLRLIAGWLQNQPPDQPSSSSTPGGFALAPAPLELDPGRRWVGTLRAELHP
                     SLDAMACSLPSWLNPLELSAGQPWEFHQPDQALVVEPPATARAEGNGVQVTGGAGRAG
                     VTLHSPRGLTSLTLSFAPTLDTLLAAGASQVLRGDQPVEAAAAFVVVEALGRSLISQP
                     QAAQAWLDDYDWQRDPDLLAIATGIVRGQRAGNAAPVRAALGRLHLIHPQLGFGRVVL
                     AAWLAGLALGVDAGTDAVALLTRPSSSDWVALELNVLNLRSVEVAGPLFGGLINALGG
                     DLPGEPVGLDLVQQAQLTGLLQLCPEEWPMARAAAACAAKNSRRLLAGVAASDFSDPD
                     DLAVLAWLALGESLV"
BASE COUNT          490 a         1144 c         1091 g          519 t
ORIGIN      
        1 caccccgatg ctggtggtgc acggcgacaa ggactaccgg gtgccgatcg gcgaagggct
       61 tcgactgtgg tgggaactgc tcagccgctc acaactcgca ctggacgaag ccggacgcac
      121 gccgcaccgc ttcctctact ttccggacga gaaccactgg gtgctgagtc cgcagcatgc
      181 cgcgatctgg tacgcgacgg tgtcggcgtt cctgtcccaa cacgtgctcg acgagccctc
      241 ggactacccg gagttgctcg gctgaaccga actacacgaa ggcttctcgc tccattcgcg
      301 gcgcgctggc acacaacggg accgttcttg atgcaggtcc gacgtcgatg tgcacccggc
      361 cccggcccag agattgataa ggggtgggtt ttggtaccga ctgctgtacc aaaacccacc
      421 cgctctcgca ggtcgtacca acggggcttc cctgaccgat gtcgcgcggt accccgagat
      481 cccgggcaag tgccggatga cgatttgcca cgggtcgaga ccctcgtggg caacggtctc
      541 gacgctcaac cgacagattg tgtgtgatca gcgctgcccc aaaacggaac cgccaccgat
      601 accggtcgcc cgaccgttag cgtggtccca tggacgggca ggagcctccg cgctcgatca
      661 gcgaacacga gtacgaggac caactcgaac cgacccacct gatccggcgc tcgcatgtag
      721 tgcttcgggc cggcacgatg atgctcgggg ccggcaccag cgcgcttcgg gtgcgtgagg
      781 tgatggccgc ggtggctcgc accgtgggcg tccagcagat cgatgcgcag gtgacgttca
      841 cccaggtggt cgtcacgatc ggacggcgcg gcatctttcg cacgcaggtg ggcgagaccc
      901 gtcccagcgt caacgccgac cggatcgccc tcctgcacca gttctcgcac gacctgcccg
      961 aacgcgagcg cgcgatcgac gtcgaggctc gactggacgc catcgaggac agcccccgcc
     1021 tctacccggc ctggatgctg gtgattctgg tcgcgttggc gtgcgcctcc gtcgccgtgc
     1081 tgagcaacgg cgggtggcgc gaggtgctcg cggtgttgcc cgcgtccgcc gtcgcctacg
     1141 ggctgcatcg gctactgacc cggctcgagt tcaacctgct ggccgtggtg ctcacctcgg
     1201 ccgcggtcgc gtccgcgctg tacgtcgccg gcgccgacct gatcacgctg gccgtcgggt
     1261 cgccgagtga ccgtttcgcg gctggtttca tctgcgcctc catctttctg attccgggct
     1321 tccccctggt caccgcgggt ttggatctga cccggctcga tctggacgcc ggcatacccc
     1381 ggctcagcta tgcggcgatg gtggtgctgg cgatcgccat cggcgtgtgg ctggtggcct
     1441 cgatcaccgg cgtgtccccg gttccggtcg gcccgatcga gggtgatccg gccctggtct
     1501 gggcggccct gatcatcgcc agtttcttcg cggtgttcgg ctgggcgacc atgttcaacg
     1561 tgccgctggg cactgcggtg gcctcgggag tggtggcgat cgtcggcaac gtgccgcggc
     1621 tgctgctgct cgagaatggc atcaaaccgc acgtggccac gttcgtcggc tgcgtgatca
     1681 tgggcctggg ctgcgcggtc gtcgcgtact ggtttcacat gaccaagatc atcatgacgg
     1741 tgcccacgct gctggtgtcg attccggggt cctcggcact gcgcacgctg atctacttcg
     1801 accaggccga catcaccgcc gcgatcgagt cgggggtctc gaccgtactg gtcgtcgtgg
     1861 cgatggtcgc cggcctgtcc ggggcccgca tgctgaccga tcccgaatgg accttcaccc
     1921 gccccgcgac cggacggacg atctgaggca gcgggcgtca gcgaccggac ggacgatctg
     1981 aggcagcggg cgtcagcacc tggcccgtcg agcgtgtcga tcagaccagc gactccccca
     2041 gcgccagcca cgccagcacc gcgagatcgt ccggatcgga gaagtccgac gccgccaccc
     2101 cggcgagcag ccggcgtgag ttcttggccg cgcacgcggc cgccgcccgc gccatcggcc
     2161 actcctcggg gcacagctgc agcagaccgg tcagctgtgc ctgctggacg aggtccaggc
     2221 ccaccggctc ccccggcagg tcgccgccca gggcgttgat caacccgccg aacagcggcc
     2281 cggccacctc cacactgcgc aggttgagca cgttgagttc gagcgccacc cagtccgaac
     2341 tgctgggtcg ggtgagcagt gcgaccgcgt cggtgccggc gtccaccccg agggccaggc
     2401 cggccagcca ggccgccagc accacacgtc cgaaacccag ctgtggatgg atcaggtgca
     2461 gccgtccgag cgccgcccgt accggtgccg cgttgcccgc gcgctgcccg cggacgatgc
     2521 cggtggcaat cgccaacagg tcggggtcgc gctgccagtc atagtcgtcc agccatgcct
     2581 gggccgcctg cggttggctg atcaggctac gtccgagtgc ttcgacgacg acgaacgccg
     2641 cggcggcttc cacgggttga tcgccacgca gtacctgcga ggctccggca gccagcaggg
     2701 tgtcgagggt cggggcgaac gacagcgtca gcgaggtgag cccacgcggt gaatgcaatg
     2761 tcacgccggc ccgtccggcc ccacccgtca cctggacgcc gttgccctca gcccgtgcgg
     2821 tcgccggcgg ctcaacgacc agcgcctggt cgggctggtg aaactcccag ggctgtccgg
     2881 cactcagttc gagcggattg agccaggacg gcaggctgca cgccatcgcg tccaggctcg
     2941 gatgcagttc ggcccgcaga gttccgaccc agcgccgtcc ggggtcgagt tcgagaggtg
     3001 ccggggccag cgcgaagccg ccgggggtcg aggaactgga gggttgatcc gggggctggt
     3061 tctgcagcca cccggcaatg agtcgcagtc cgaccaccgg accggcgcgg tgccggggcg
     3121 ccacgcacag cacaccctca gcacctgccg cccaaaccca gaccaccgcg gccggctcgg
     3181 gacgcacacg caactccggc gccgcgacac tgcaccacct gtcgtccacc gggtcgcgga
     3241 cgag
//
LOCUS       DAOSUG010000366         3235 bp    DNA     linear   ENV 10-JUN-2024
DEFINITION  MAG TPA_asm: Micropruina sp. isolate AMR_MDS_2195
            NODE_12151_length_3235_cov_4.307233, whole genome shotgun sequence.
ACCESSION   DAOSUG010000366 DAOSUG010000000
VERSION     DAOSUG010000366.1
DBLINK      BioProject: PRJNA925477
            BioSample: SAMN32864262
            Sequence Read Archive: SRR4236673
KEYWORDS    WGS; ENV; Metagenome Assembled Genome; MAG; Third Party Data; TPA;
            TPA:assembly.
SOURCE      Micropruina sp. (wastewater metagenome)
  ORGANISM  Micropruina sp.
            Bacteria; Bacillati; Actinomycetota; Actinomycetes;
            Propionibacteriales; Nocardioidaceae; Micropruina.
REFERENCE   1  (bases 1 to 3235)
  AUTHORS   Abdulkadir,N., Saraiva,J.P., Zhang,J., Stolte,S., Gillor,O.,
            Harms,H. and Rocha,U.
  TITLE     Genome-centric analyses of 165 metagenomes show that mobile genetic
            elements are crucial for the transmission of antimicrobial
            resistance genes to pathogens in activated sludge and wastewater
  JOURNAL   Microbiol Spectr 12 (3), e0291823 (2024)
   PUBMED   38289113
REFERENCE   2  (bases 1 to 3235)
  AUTHORS   Abdulkadir,N.
  TITLE     Direct Submission
  JOURNAL   Submitted (24-MAR-2023) Environmental Microbiology, Helmholtz
            Center for Environmental Research -UFZ, Permoserstr 15, Leipzig
            04318, Germany
COMMENT     The annotation was added by the NCBI Prokaryotic Genome Annotation
            Pipeline (PGAP). Information about PGAP can be found here:
            https://www.ncbi.nlm.nih.gov/genome/annotation_prok/
            
            ##Genome-Assembly-Data-START##
            Assembly Method        :: SPAdes v. 3.11.1
            Genome Representation  :: Full
            Expected Final Version :: Yes
            Genome Coverage        :: Not Applicable
            Sequencing Technology  :: Illumina MiSeq
            ##Genome-Assembly-Data-END##
            
            ##Genome-Annotation-Data-START##
            Annotation Provider               :: NCBI
            Annotation Date                   :: 05/04/2023 04:57:10
            Annotation Pipeline               :: NCBI Prokaryotic Genome
                                                 Annotation Pipeline (PGAP)
            Annotation Method                 :: Best-placed reference protein
                                                 set; GeneMarkS-2+
            Annotation Software revision      :: 6.5
            Features Annotated                :: Gene; CDS; rRNA; tRNA; ncRNA
            Genes (total)                     :: 2,776
            CDSs (total)                      :: 2,750
            Genes (coding)                    :: 2,714
            CDSs (with protein)               :: 2,714
            Genes (RNA)                       :: 26
            tRNAs                             :: 24
            ncRNAs                            :: 2
            Pseudo Genes (total)              :: 36
            CDSs (without protein)            :: 36
            Pseudo Genes (ambiguous residues) :: 0 of 36
            Pseudo Genes (frameshifted)       :: 2 of 36
            Pseudo Genes (incomplete)         :: 32 of 36
            Pseudo Genes (internal stop)      :: 2 of 36
            ##Genome-Annotation-Data-END##
FEATURES             Location/Qualifiers
     source          1..3235
                     /organism="Micropruina sp."
                     /mol_type="genomic DNA"
                     /submitter_seqid="NODE_12151_length_3235_cov_4.307233"
                     /isolate="AMR_MDS_2195"
                     /isolation_source="Activated sludge of municipal
                     wastewater treatment plants"
                     /db_xref="taxon:2737536"
                     /environmental_sample
                     /geo_loc_name="Canada"
                     /lat_lon="49.25 N 123.1 W"
                     /collection_date="2015-06-16"
                     /metagenome_source="wastewater metagenome"
                     /note="metagenomic"
     gene            <1..2927
                     /gene="eccCb"
                     /locus_tag="PLF56_11145"
     CDS             <1..2927
                     /gene="eccCb"
                     /locus_tag="PLF56_11145"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_014847203.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_process: GO:0009306 - protein secretion [Evidence IEA]"
                     /codon_start=3
                     /transl_table=11
                     /product="type VII secretion protein EccCb"
                     /protein_id="HOQ54154.1"
                     /translation="AAGLPGGGASYAELGVTVLHLLADRLDEPTEVDLRLTMAGDTAV
                     AEARPGTPEMTSRTLTPDALGSADFAGTARALAALRLSQVAAREDRAETVMDLAGLLG
                     VARPDDIDVERLWRPRGPADFLRVPFGVDDAGNRVYLDLKESAQFGMGPHGICIGATG
                     SGKSEMLRTLILSLAMAHPPDDLSMILVDYKGGAAFAPFAGLPHLAGLIDNLADDPQL
                     TTRARASIQGEVVRRQQLLKDAGSSPSITHYRQLRAERPDLPPLPHLFVVIDEFGELL
                     TAEPDFIDLFLQIGRIGRSIGVHLLLSSQRIEGGKLRGLDTYLSYRLGLRTFSESESS
                     VVLNTTDAFHLPALPGYGYLKVDTTVYTRFRAGYVSGPAQQQQHVRALDVAPRPYLLP
                     AYNGIRTADEHGDGGERPLQLHRPDTGVTFVEASVQQLRDDDRLTRPIWLPPLPERVA
                     LGSLISDDTRLPPLTAVLGLTDDPAKQSQTPLLVDLTATGGHLVIIGAPQSGRSTVLR
                     TLAVSLCLTRTPREVALYGMDLTGGGLQRLEGFPHVGGVVTRGSRDRLRRLLEEVSVM
                     LVQREAVFAERGIDSLSQLRTLHAQGRLPELPAADIVLLVDGFGALRTDFDELEELFT
                     DIMQRAAGYGIHLVLTMTRFNELRLAHQALFGNKIELRLNDPADSSIDRKLSAVLPKD
                     SPGRALLPEKNLAQIALPVLDEVPDEEVADELQRLARQASRSWSGPAAAPIRLLPLEL
                     SPIELPDRFDEPDAVPIGLRQDTMEAALWDFLGEDQHLVVFGDTKCGKTSLLRTIAAG
                     LMDRYTPDELALAVIDIRGHVPSAIDEAYLAAHARNAQQARGLASSIAAELEQRPNRS
                     PEEQARSPRVVVLVDDYDIVSAGGTDPLGPLLPYLPSARDLGLHLIITRPVAGASRAM
                     YDRVLQVTRDTGGSMLLMSGERSEGQLLPRVYPERFPPGRGRFIRRGERPHIVQVALP
                     PR"
BASE COUNT          458 a         1193 c         1118 g          466 t
ORIGIN      
        1 gagccgccgg cctgcccggc ggcggcgcga gctatgccga gctgggcgtc accgtgctgc
       61 acctgctcgc cgaccggctg gacgagccca ccgaggtcga cctgcggctt accatggcgg
      121 gcgacacggc ggtggcggag gcacgtccgg gcacgcccga gatgacctcg cgcaccctca
      181 cccccgacgc cctcggctcg gccgatttcg ccggaacagc gcgggcgctg gcggcgctgc
      241 ggctgtcgca ggtcgccgcg cgcgaagacc gggccgagac cgtgatggac ctggccggcc
      301 tgctgggcgt ggcccgaccc gacgacatcg atgtggagcg gctgtggcgg ccgcgcggcc
      361 cggccgactt tctgcgggtg ccgttcgggg tggacgatgc cggcaaccgg gtgtacctcg
      421 acctgaaaga gagcgcgcag ttcggcatgg gtccgcacgg catctgcatc ggcgcgaccg
      481 gatcgggcaa gtccgagatg ctgcgcacgc tgatcctgtc actggcgatg gcgcatccgc
      541 ccgacgacct gagcatgatc ctggtcgact acaagggcgg cgccgcgttc gcacccttcg
      601 ccgggctgcc gcacctggcc gggctgatcg acaacctggc cgacgacccg cagctcacca
      661 cgcgcgcgcg ggcgtccatt cagggcgagg tggtgcgccg ccagcagttg ctcaaggacg
      721 ccggctcgtc cccctccatc acccactacc gccagctgcg cgcggagcgt ccggacctgc
      781 cgccgctgcc gcacctgttc gtggtgatcg acgagttcgg cgagctgctg accgcagaac
      841 ccgacttcat cgacctgttc ctgcagatcg gacgcatcgg ccgttccatc ggcgtgcacc
      901 tgctgctgag cagccagcgg atcgagggcg gcaagctgcg cggcctcgac acctacctca
      961 gctacaggct cggcctgcgc acgttcagcg agtcggagtc gtcggtggtg ctcaacacca
     1021 ccgacgcctt tcacctgccc gcgctgcccg gctacggcta tctcaaggtc gacaccacgg
     1081 tgtacacccg gttccgggcg ggctacgtgt cgggcccggc gcagcagcag cagcacgtgc
     1141 gggcgctgga cgtcgccccc cgcccctacc tactgcccgc ctacaacggc atccgtactg
     1201 cggacgagca cggcgacggc ggcgagcggc cgctgcagtt gcaccgtccg gacaccgggg
     1261 tgacgttcgt ggaggcgtcc gtgcagcaac tgcgcgacga tgaccggctg acccgtccga
     1321 tctggctgcc gccgctgccc gaacgggtcg cgctgggctc gctgatcagc gacgacaccc
     1381 ggctgccgcc gctgacggcg gtgctggggc tgaccgacga ccccgccaaa cagtcgcaga
     1441 cccccctgct ggtcgacctg accgcgaccg gcggtcacct ggtcatcatc ggggcccccc
     1501 agtccggacg ctccaccgtg ctgcgcacgc tggccgtgtc gctgtgcctc acccggacgc
     1561 cgcgcgaggt cgccctgtac ggcatggacc tgaccggtgg cggcctgcaa cggctggaag
     1621 ggtttccgca tgtcggcggg gtggtcacca ggggcagccg cgaccgcctg cgacggttgc
     1681 tcgaggaggt gtcggtgatg ctggtgcagc gcgaggcggt gttcgccgaa cgcggtatcg
     1741 actcactttc gcagctgcgc accctgcacg cgcagggccg gctgcccgaa ctgccggcgg
     1801 ccgacatcgt gctgctggtc gacggtttcg gcgcgctgcg taccgacttc gacgagctcg
     1861 aggagctgtt caccgacatc atgcaacggg ccgccggcta cggcattcac ctggtgctca
     1921 cgatgacccg gttcaacgag ctgcggctgg cgcaccaggc cctgttcggc aacaagatcg
     1981 aactgcgact gaacgatccg gccgactcga gcatcgaccg caagctgtcg gcggtgctcc
     2041 ccaaggattc gcccggccgc gccctgctgc ccgagaagaa cctggcccag atcgcgctgc
     2101 cggtgctcga tgaggtaccg gacgaagagg tcgccgacga gttgcagcgg ctggcccggc
     2161 aggcgtcccg gtcgtggagc ggcccggcgg cggcaccgat ccggctgctg cccctcgagc
     2221 tgtcgccgat cgaactgccc gaccggttcg acgagccgga cgcggtgccg atcggcctgc
     2281 gccaggacac catggaggcc gccctgtggg acttcctcgg cgaggaccag cacctggtgg
     2341 tgttcgggga caccaagtgc ggcaagacgt ccctgctccg cacgatcgcc gccggactga
     2401 tggaccgcta cacccccgac gaactggcgc tggcggtgat cgacattcgg gggcacgtgc
     2461 cgtccgcgat cgatgaggcg tacctggccg cacacgcccg caacgcgcag caggcgcgcg
     2521 ggctggcgtc gtccatcgcc gcggaactgg agcagcggcc caaccgcagc cccgaggagc
     2581 aggcccgctc gccgcgcgtg gtggtgctgg tcgatgacta cgacatcgtc agcgccggcg
     2641 gcacggaccc gctcgggcca ctgctgccct acctgcccag cgcccgcgat ctcggcctgc
     2701 acctgatcat cacccgcccc gtcgccggcg ccagccgcgc catgtacgac cgggtgctgc
     2761 aggtcacccg cgacaccggc ggctcgatgc tgctgatgtc gggcgaacgc agcgagggcc
     2821 agctgctccc ccgcgtctac ccggaacgct tccccccggg tcgtggccgg ttcatcagac
     2881 gcggcgagcg accgcacatc gtgcaggtcg ccctgccccc gcgctgatgg gccccgcccg
     2941 cggccgcggc caccacccgt ccgaggcttg gttgccgttt tgacgcctca ccgcctgcgc
     3001 gcaggcagtt gggtgccacg aaggcggcta cctggtagtc acgtgccacc agggcagcga
     3061 cctgtgcgga tgccggcgcg ccccagcccg tccccgggct cacgagcgcg gcggaaagat
     3121 cccgttcagc gcgatgcacc agttgagcgc caggtacggc ggacggttct cgtgcggctg
     3181 gccaccctgg ttggccgcga tcattccctg gttcatcgcg acgccggggg tggcg
//
LOCUS       DAOSUG010000367         3234 bp    DNA     linear   ENV 10-JUN-2024
DEFINITION  MAG TPA_asm: Micropruina sp. isolate AMR_MDS_2195
            NODE_12154_length_3234_cov_5.241900, whole genome shotgun sequence.
ACCESSION   DAOSUG010000367 DAOSUG010000000
VERSION     DAOSUG010000367.1
DBLINK      BioProject: PRJNA925477
            BioSample: SAMN32864262
            Sequence Read Archive: SRR4236673
KEYWORDS    WGS; ENV; Metagenome Assembled Genome; MAG; Third Party Data; TPA;
            TPA:assembly.
SOURCE      Micropruina sp. (wastewater metagenome)
  ORGANISM  Micropruina sp.
            Bacteria; Bacillati; Actinomycetota; Actinomycetes;
            Propionibacteriales; Nocardioidaceae; Micropruina.
REFERENCE   1  (bases 1 to 3234)
  AUTHORS   Abdulkadir,N., Saraiva,J.P., Zhang,J., Stolte,S., Gillor,O.,
            Harms,H. and Rocha,U.
  TITLE     Genome-centric analyses of 165 metagenomes show that mobile genetic
            elements are crucial for the transmission of antimicrobial
            resistance genes to pathogens in activated sludge and wastewater
  JOURNAL   Microbiol Spectr 12 (3), e0291823 (2024)
   PUBMED   38289113
REFERENCE   2  (bases 1 to 3234)
  AUTHORS   Abdulkadir,N.
  TITLE     Direct Submission
  JOURNAL   Submitted (24-MAR-2023) Environmental Microbiology, Helmholtz
            Center for Environmental Research -UFZ, Permoserstr 15, Leipzig
            04318, Germany
COMMENT     The annotation was added by the NCBI Prokaryotic Genome Annotation
            Pipeline (PGAP). Information about PGAP can be found here:
            https://www.ncbi.nlm.nih.gov/genome/annotation_prok/
            
            ##Genome-Assembly-Data-START##
            Assembly Method        :: SPAdes v. 3.11.1
            Genome Representation  :: Full
            Expected Final Version :: Yes
            Genome Coverage        :: Not Applicable
            Sequencing Technology  :: Illumina MiSeq
            ##Genome-Assembly-Data-END##
            
            ##Genome-Annotation-Data-START##
            Annotation Provider               :: NCBI
            Annotation Date                   :: 05/04/2023 04:57:10
            Annotation Pipeline               :: NCBI Prokaryotic Genome
                                                 Annotation Pipeline (PGAP)
            Annotation Method                 :: Best-placed reference protein
                                                 set; GeneMarkS-2+
            Annotation Software revision      :: 6.5
            Features Annotated                :: Gene; CDS; rRNA; tRNA; ncRNA
            Genes (total)                     :: 2,776
            CDSs (total)                      :: 2,750
            Genes (coding)                    :: 2,714
            CDSs (with protein)               :: 2,714
            Genes (RNA)                       :: 26
            tRNAs                             :: 24
            ncRNAs                            :: 2
            Pseudo Genes (total)              :: 36
            CDSs (without protein)            :: 36
            Pseudo Genes (ambiguous residues) :: 0 of 36
            Pseudo Genes (frameshifted)       :: 2 of 36
            Pseudo Genes (incomplete)         :: 32 of 36
            Pseudo Genes (internal stop)      :: 2 of 36
            ##Genome-Annotation-Data-END##
FEATURES             Location/Qualifiers
     source          1..3234
                     /organism="Micropruina sp."
                     /mol_type="genomic DNA"
                     /submitter_seqid="NODE_12154_length_3234_cov_5.241900"
                     /isolate="AMR_MDS_2195"
                     /isolation_source="Activated sludge of municipal
                     wastewater treatment plants"
                     /db_xref="taxon:2737536"
                     /environmental_sample
                     /geo_loc_name="Canada"
                     /lat_lon="49.25 N 123.1 W"
                     /collection_date="2015-06-16"
                     /metagenome_source="wastewater metagenome"
                     /note="metagenomic"
     gene            294..749
                     /locus_tag="PLF56_11150"
     CDS             294..749
                     /locus_tag="PLF56_11150"
                     /inference="COORDINATES: ab initio
                     prediction:GeneMarkS-2+"
                     /note="Derived by automated computational analysis using
                     gene prediction method: GeneMarkS-2+."
                     /codon_start=1
                     /transl_table=11
                     /product="hypothetical protein"
                     /protein_id="HOQ54155.1"
                     /translation="MGRPRAKVKRGFGEVGQLPSGRYRARYTGPDGSRHSAPITFVAR
                     IDAEGWLVDQERMISRGEWEPPARQVREVHEAPLLLGEYAATVVARRRIRPGTAVLFA
                     KLLRLIILPAFAKRPLAEITTAEISTWYAAMRSTPTQQANAYGLVKSIF"
     gene            771..1118
                     /locus_tag="PLF56_11155"
     CDS             771..1118
                     /locus_tag="PLF56_11155"
                     /inference="COORDINATES: ab initio
                     prediction:GeneMarkS-2+"
                     /note="Derived by automated computational analysis using
                     gene prediction method: GeneMarkS-2+."
                     /codon_start=1
                     /transl_table=11
                     /product="hypothetical protein"
                     /protein_id="HOQ54156.1"
                     /translation="MSPCRVKAGTQKERAREIEVLAVDQLNRYLAAVPEARRVPLFPA
                     RDGHSPLNDSVLQVAHNKGKRPSTRAQLGFTRCVLAAKGEVHSSRSMRRRSLASHTSA
                     STLAMNTVGAYWR"
     gene            complement(1653..2327)
                     /locus_tag="PLF56_11160"
     CDS             complement(1653..2327)
                     /locus_tag="PLF56_11160"
                     /inference="COORDINATES: protein motif:HMM:NF014906.2"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_function: GO:0003677 - DNA binding [Evidence IEA];
                     GO_process: GO:0006355 - regulation of DNA-templated
                     transcription [Evidence IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="TetR/AcrR family transcriptional regulator"
                     /protein_id="HOQ54157.1"
                     /translation="MSAEGEHDVTSGAGLSRRRVVLEAIRLADQEGIEGLSMRRLAGA
                     LGAGAMSLYHYVKSRDELLDAMIDLVFEEIELPPEDTDWQTAMRRRSESARQVLARHR
                     WAIVLMDSRTSPGPAHLHHREAVTACLRRAGFPVLMTTHATWLLDSYVYGFALQEASL
                     PFDSAHELANMVEDVYLPQLPPDQFPYLNESAAALVAADYDPADQFAFGLELVLAALD
                     PLRADA"
     gene            2439..>3234
                     /locus_tag="PLF56_11165"
     CDS             2439..>3234
                     /locus_tag="PLF56_11165"
                     /inference="COORDINATES: protein motif:HMM:NF012335.2"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="NAD(P)-dependent alcohol dehydrogenase"
                     /protein_id="HOQ54158.1"
                     /translation="MRTGQQPNSGARVEHGTGPAGAPTMRAVVQHRYGPLPVLGLAEV
                     GIPSPGRGEVLVQVGAASVHPGDYYVMTGEPYVVRLVFGLRRPRHGILGRDLAGVVSA
                     VGEDVTALRPGDRVFGWSAAGAFAEYACIPADNLVPVPANLSVVSAAAVPTSAMTALQ
                     ALRDVARIRPGQTVLVTGASGGVGSFAVQIAKAFGAEVTGVCSTRNVDLVRSLGADHV
                     IDYTATDFTHGHERYDVILDNVEASPLAAVRRALTPVGTLIPNSGRG"
BASE COUNT          507 a         1106 c         1096 g          525 t
ORIGIN      
        1 ggttcaggtc cgtgtgtccg aaaggacgtg gaggttcaac tcctctctcg cgcacaacga
       61 tgtatggtcc cctgactaca tcaaatcccc tctgacaagg acttcggtcc ttctcagttg
      121 tggactccca tggacacgga tggccttcag aggcctccca ttccccacgt attccccacg
      181 gttccccacg tattccccac gacattcggg gcgtggggaa cgttgtgagg gtcgccccct
      241 gtccacccga ccgtccttga gcccgttacg gagcgtgacg ggaggggcgc atcatgggta
      301 gaccacgggc caaggtcaag cgcggcttcg gtgaggtcgg ccagctgccg agcgggcggt
      361 accgggcgcg ctacaccggc cccgacggca gccggcacag cgctccgatc accttcgtcg
      421 cccggatcga cgccgagggg tggctggtcg accaggaacg gatgatcagc cgcggcgagt
      481 gggaaccgcc ggcccgtcag gtccgggagg ttcacgaggc acccctgctg ctgggtgagt
      541 acgccgccac cgtggtggcc cgccggcgaa ttcgaccggg cacggcggtg ctgttcgcga
      601 agttgctccg attgatcatt cttccggcgt tcgccaagcg tcccttggcc gagatcacca
      661 ccgcggagat ctccacctgg tacgccgcga tgcggtcgac gcccacccag caggccaacg
      721 cctacgggct ggtcaagtcg atcttttaag ttaagggagc agtcgaggag gtgagcccct
      781 gccgggtcaa ggccggcacc cagaaggaac gcgcccgcga gatcgaggtg ctcgccgtcg
      841 accagctcaa ccgctacctg gctgccgtgc ccgaggcgcg gcgggttccg cttttcccgg
      901 cccgggacgg gcactccccg ctcaacgaca gcgtcctgca ggtcgcccac aacaagggca
      961 agcggccatc gacacgcgcg cagctcggct tcacgcgctg cgtcctcgcg gccaagggtg
     1021 aggtccactc gtcgaggtcg atgaggcgcc gctccttggc gagccacacc tcggcgtcca
     1081 ccttggccat gaacacggtc ggggcgtact ggcgctgatc gccggggccg acgtagctgg
     1141 cgtggtaacc gctacggagt ctcttgatcg ctcttcaggt ccggcgccgc tgggcgccca
     1201 tactgctggt agcagtagcc gtccgcattc ttgctcttcg ctgctgtgag tccgcaccgg
     1261 gagctgtcgg ccttgatcat gcggcaccgg gtgccggggt cgttcggcac cccacgtagc
     1321 acatcgacct cccgaattcc gttcccttag cagtccgaag acagcctacg tcagcccact
     1381 tcgcgactcg aaggatgaat gcagcggagc actaaactgc ctagtcagag agccgtaaag
     1441 cgcctcaatc gggctagatg tcacagaccc cgcactcacg agttcgaatc tcgtatcctc
     1501 tgccagttga cgagggatcc accatcggga acctctcgcg cgtggagagt gggcgaggtg
     1561 tagtcccgat tcccgacccg gtcagcagtc cgcggtgcct tcctctgggt caggacgcac
     1621 ccagacggac gccggcgtgg attgcggggt ggctatgcgt cggctcgcag gggatcgagg
     1681 gcggcgagca cgagctcgag gccgaacgcg aactgatccg ccgggtcgta gtcggcggcg
     1741 acgagcgccg cggcggactc gttgaggtag gggaactggt caggcgggag ctggggaagg
     1801 tagacgtcct cgaccatgtt cgcgagctca tgggctgagt cgaacggcag gctggcttcc
     1861 tgcagggcga aaccgtagac ataactgtcg agcagccagg tggcgtgggt cgtcatcagg
     1921 actgggaagc cggccctgcg caggcatgcg gtgacggctt cgcggtggtg gaggtgcgca
     1981 ggccccggtg atgttcgcga gtccatcagg acgatcgccc accggtggcg ggcgaggacc
     2041 tgccgggcgg actccgaccg ccgtcgcatc gccgtctgcc agtcggtgtc ctcggggggg
     2101 agctcgatct cctcgaacac gagatcgatc atggcgtcca ggagttcgtc cctgctcttc
     2161 acgtagtggt agagcgacat cgcgcccgcg cccagcgcgc cggccagccg gcgcatgctc
     2221 agcccttcga tcccctcctg atcggccagc cggatggcct cgagcaccac ccgccgcctg
     2281 ctcagccccg cgcccgacgt gacgtcgtgc tctccctcgg cagacacagt cctcctcgct
     2341 cgtacggctt gacgatcgta cagcgtacgt gcatagtaca gcgtacgacg tacagtgtac
     2401 gagtcgcggg accgcgactc ggacgtgagg aggcctcgat gagaaccgga cagcagccga
     2461 acagcggcgc acgcgtggag cacgggacgg gtccggccgg cgcgccgacg atgcgggccg
     2521 tcgtccagca ccgctacggc ccgctcccgg tgctggggtt ggccgaggtc ggcatcccgt
     2581 cgcccggacg cggcgaggtg ctcgtccagg tgggcgcggc gtcggtccat ccgggcgact
     2641 actacgtcat gaccggtgag ccgtacgtgg tccgcctggt gttcgggctc cgccgaccgc
     2701 gtcacggcat cctcggcagg gacctcgccg gcgtggtgtc agcggtcggg gaggacgtca
     2761 ctgcgctgcg tcccggcgac cgggtgttcg gctggagcgc cgccggagcg ttcgccgagt
     2821 acgcctgcat ccctgcggac aacctcgtgc ccgtgcccgc caacctctcg gtcgtgagcg
     2881 cagcagcggt accgacctcg gccatgacgg cgctccaggc cctgcgcgac gtcgcgagga
     2941 tccggccggg ccagaccgtc ctggtcacgg gcgcgtcggg cggcgtgggc tccttcgccg
     3001 ttcagatcgc caaggcgttc ggtgccgagg tgacgggggt gtgcagcacc cgcaacgtcg
     3061 acctcgttcg ctcgctcggg gccgaccacg tcatcgacta cacggcgacc gacttcaccc
     3121 acggccacga gcgctacgac gtgatcctcg acaacgtgga agccagcccc ctcgcggccg
     3181 tccgccgggc gctgacgccg gtcggcaccc tcatccccaa cagcggacgc ggcg
//
LOCUS       DAOSUG010000368         3228 bp    DNA     linear   ENV 10-JUN-2024
DEFINITION  MAG TPA_asm: Micropruina sp. isolate AMR_MDS_2195
            NODE_12186_length_3228_cov_4.426410, whole genome shotgun sequence.
ACCESSION   DAOSUG010000368 DAOSUG010000000
VERSION     DAOSUG010000368.1
DBLINK      BioProject: PRJNA925477
            BioSample: SAMN32864262
            Sequence Read Archive: SRR4236673
KEYWORDS    WGS; ENV; Metagenome Assembled Genome; MAG; Third Party Data; TPA;
            TPA:assembly.
SOURCE      Micropruina sp. (wastewater metagenome)
  ORGANISM  Micropruina sp.
            Bacteria; Bacillati; Actinomycetota; Actinomycetes;
            Propionibacteriales; Nocardioidaceae; Micropruina.
REFERENCE   1  (bases 1 to 3228)
  AUTHORS   Abdulkadir,N., Saraiva,J.P., Zhang,J., Stolte,S., Gillor,O.,
            Harms,H. and Rocha,U.
  TITLE     Genome-centric analyses of 165 metagenomes show that mobile genetic
            elements are crucial for the transmission of antimicrobial
            resistance genes to pathogens in activated sludge and wastewater
  JOURNAL   Microbiol Spectr 12 (3), e0291823 (2024)
   PUBMED   38289113
REFERENCE   2  (bases 1 to 3228)
  AUTHORS   Abdulkadir,N.
  TITLE     Direct Submission
  JOURNAL   Submitted (24-MAR-2023) Environmental Microbiology, Helmholtz
            Center for Environmental Research -UFZ, Permoserstr 15, Leipzig
            04318, Germany
COMMENT     The annotation was added by the NCBI Prokaryotic Genome Annotation
            Pipeline (PGAP). Information about PGAP can be found here:
            https://www.ncbi.nlm.nih.gov/genome/annotation_prok/
            
            ##Genome-Assembly-Data-START##
            Assembly Method        :: SPAdes v. 3.11.1
            Genome Representation  :: Full
            Expected Final Version :: Yes
            Genome Coverage        :: Not Applicable
            Sequencing Technology  :: Illumina MiSeq
            ##Genome-Assembly-Data-END##
            
            ##Genome-Annotation-Data-START##
            Annotation Provider               :: NCBI
            Annotation Date                   :: 05/04/2023 04:57:10
            Annotation Pipeline               :: NCBI Prokaryotic Genome
                                                 Annotation Pipeline (PGAP)
            Annotation Method                 :: Best-placed reference protein
                                                 set; GeneMarkS-2+
            Annotation Software revision      :: 6.5
            Features Annotated                :: Gene; CDS; rRNA; tRNA; ncRNA
            Genes (total)                     :: 2,776
            CDSs (total)                      :: 2,750
            Genes (coding)                    :: 2,714
            CDSs (with protein)               :: 2,714
            Genes (RNA)                       :: 26
            tRNAs                             :: 24
            ncRNAs                            :: 2
            Pseudo Genes (total)              :: 36
            CDSs (without protein)            :: 36
            Pseudo Genes (ambiguous residues) :: 0 of 36
            Pseudo Genes (frameshifted)       :: 2 of 36
            Pseudo Genes (incomplete)         :: 32 of 36
            Pseudo Genes (internal stop)      :: 2 of 36
            ##Genome-Annotation-Data-END##
FEATURES             Location/Qualifiers
     source          1..3228
                     /organism="Micropruina sp."
                     /mol_type="genomic DNA"
                     /submitter_seqid="NODE_12186_length_3228_cov_4.426410"
                     /isolate="AMR_MDS_2195"
                     /isolation_source="Activated sludge of municipal
                     wastewater treatment plants"
                     /db_xref="taxon:2737536"
                     /environmental_sample
                     /geo_loc_name="Canada"
                     /lat_lon="49.25 N 123.1 W"
                     /collection_date="2015-06-16"
                     /metagenome_source="wastewater metagenome"
                     /note="metagenomic"
     gene            305..919
                     /locus_tag="PLF56_11170"
     CDS             305..919
                     /locus_tag="PLF56_11170"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_000904941.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_function: GO:0000150 - DNA strand exchange activity
                     [Evidence IEA];
                     GO_function: GO:0003677 - DNA binding [Evidence IEA];
                     GO_process: GO:0006310 - DNA recombination [Evidence IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="recombinase family protein"
                     /protein_id="HOQ54159.1"
                     /translation="MLVGYMRVSKADGSQTTDLQRDALLIAGVDPEALYEDKASGKKD
                     DRPQLTGCLKALRADDTLLVWKLDRLGRDLRHLVNIVHDLTQRGIGLKVLTGQGAAID
                     TTTASGKLVFGIFAALAEYERELISERTIAGLAAARARGRKGGRPFKMTPAKVRLAAA
                     SMGKPGTNVGELCKELGITRQTLYRHVSPTGELRDDGHKILDRR"
     gene            complement(1252..1788)
                     /locus_tag="PLF56_11175"
     CDS             complement(1252..1788)
                     /locus_tag="PLF56_11175"
                     /inference="COORDINATES: ab initio
                     prediction:GeneMarkS-2+"
                     /note="Derived by automated computational analysis using
                     gene prediction method: GeneMarkS-2+."
                     /codon_start=1
                     /transl_table=11
                     /product="hypothetical protein"
                     /protein_id="HOQ54160.1"
                     /translation="MYVERYGHVDAWVASTPIADMVAGLQQALPAVIRRRPDGAPQRF
                     LVSLAARLNPVRAITLAKYERQFGADGIHGALRVLKAVGPVDLARIDAPLLALVGASE
                     DAEFRRQAYQVHQAVRQRRPESRLIEFAPDTGADAHCQVNNLPLALAHTFAWLNDIGL
                     AAAGDVDRFVAESPHTRP"
     gene            2102..2656
                     /locus_tag="PLF56_11180"
     CDS             2102..2656
                     /locus_tag="PLF56_11180"
                     /EC_number="2.-.-.-"
                     /inference="COORDINATES: protein motif:HMM:NF024698.2"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="GNAT family protein"
                     /protein_id="HOQ54161.1"
                     /translation="MPSFADEVIKSHGVVLRPLTEADVPAIAIACSDAQTQQWLPLPR
                     PYTEDAARSYALEVAPAALASGRALERAIELNGEFAGVIGLKNTDWAVAKTEAGYWLG
                     PWARGRGIAARALDAITDWALDSQGIERVEVLVATGNAASLATARKAKFIEEGTLRRA
                     GYTHDGKVDLVILSRLAHDPSPRL"
     gene            complement(2703..3017)
                     /locus_tag="PLF56_11185"
     CDS             complement(2703..3017)
                     /locus_tag="PLF56_11185"
                     /inference="COORDINATES: ab initio
                     prediction:GeneMarkS-2+"
                     /note="Derived by automated computational analysis using
                     gene prediction method: GeneMarkS-2+."
                     /codon_start=1
                     /transl_table=11
                     /product="hypothetical protein"
                     /protein_id="HOQ54162.1"
                     /translation="MTDIDDVWPRIEQHARAQRELRELLNEWDPIGVFDLADVDQGSW
                     PVDEYDCIREPLISHLLRGDNRSEIAEFLRNELTSHFGLEPWLVTTNVVERIFDWWES
                     VR"
BASE COUNT          557 a         1085 c         1052 g          534 t
ORIGIN      
        1 gacgccgaag ccctcagccc caccccacta cgcactgccc tgcacgaccg cgcccaggca
       61 atcgccgacc gctacgcctg acccggggaa gcggccagca cccagccctc cggttcccag
      121 cagccataat gcgccgcgac cggatcggcc tacgggaacc ctccgggcaa cccgggccgg
      181 tgcaccgcca gagagcgcag cgagaacccc gccatctacc gctgaaagtc acacgcatgg
      241 cgagctggtg tcccgctgag actgtcacgc aaacggtcgt atgcgtgacg cggatgggag
      301 agagatgctg gtcggctaca tgcgggtctc gaaggccgac gggtcgcaga ccaccgacct
      361 gcaacgcgac gccctcctca tcgctggcgt cgaccctgag gcactgtatg aggacaaggc
      421 ctccggcaag aaggacgacc gcccccagct gaccggctgc ctgaaagccc tccgcgccga
      481 cgacaccctg ctggtgtgga agctcgaccg gctcggtcga gacctgcgcc acctggtcaa
      541 catcgtccac gacctcaccc aacgcggcat cggcctgaaa gtcctcaccg ggcagggggc
      601 ggccatcgac accactaccg cctccggcaa gctggtgttc ggcatcttcg ccgctctggc
      661 cgagtacgaa cgcgagctga tctctgaacg caccatcgcc ggcctggctg ccgcccgtgc
      721 ccgcggccgt aagggcggca ggccgttcaa gatgacgccc gccaaagttc gcctcgctgc
      781 cgcatcgatg ggcaagccag gcaccaacgt cggtgagctg tgcaaggaac tcggcatcac
      841 ccgccaaacc ctctaccggc acgtctcacc caccggcgaa ctccgcgacg acggccacaa
      901 gatcctcgac cgtcggtgac gcaaggcccg gtccggctgc gtacgaccca ggtcgtggcg
      961 ccgctgacct gcaccgctcc caacatcacg ttgggcttgt gggatgccgt cggcaggcga
     1021 agatgaaaac gaggggcgtc ggcctgtgac tcctggtggc tagcgccggg cggtgcggga
     1081 gccgtgcccg gttcgtctgc agaatgctct ttcagcgctc ggccgcggcc cagaacgtgt
     1141 cgaggagtgt gtcgtcgact gggtcgagac gggcagggct cgtcgctgag cctccaaggg
     1201 cgctggtccg caatctgcgg acagtcgtcc tcgatctggg cggtcctcgg ttcacgggcg
     1261 ggtgtgcggt gattctgcga cgaagcggtc cacgtccccg gccgccgcca ggccgatgtc
     1321 gttgagccat gcgaaggtgt gagcgagggc gagtggcagg ttgttgacct ggcagtgggc
     1381 gtcggcgccg gtgtcgggag cgaactcgat cagccgggat tcgggtcgac gttgccggac
     1441 ggcctgatgc acctggtacg cctgccggcg gaactctgca tcctcgctgg ccccaaccag
     1501 ggccagcagc ggggcgtcga tccgggccag gtcgacggga ccgaccgcct tgaggacccg
     1561 cagtgcgccg tggatgccgt cggcgccgaa ctgtcgctcg tacttggcca gggtgatcgc
     1621 ccggaccggg ttgagccggg ctgcgaggct gaccaggaac cgttggggtg caccgtcggg
     1681 acggcgccga atcacggcag gcagggcctg ttggaggccc gcgaccatgt ccgcgatcgg
     1741 agtcgaggca acccaggcgt cgacgtggcc gtaccgttcg acgtacatgg cggtgaacag
     1801 gccgccaccg ctccacccga ccaggatggt gcggccacga tggccgcgtg cccgggggag
     1861 gtgtggcgtt ggggtgtgcc ccagcgccct tgtgcaacac ctacaacttg gtgacggtgc
     1921 cgatgaaaaa cgcgccgccc tacggacttc ccgatggtcg tgaactcact caactgccca
     1981 gagtcggtcg tgtgttgacg cccagggtcg tgatctcgct caactgccga tgtgcggtct
     2041 ttcaccgctt gagactatga tccagcagca gccggcaatc acctgtcact aggatccgtg
     2101 catgccgtcc ttcgcagatg aagtcatcaa gtcccatggg gtcgtcctgc ggccgctgac
     2161 cgaggccgac gtgcctgcaa tcgcgatcgc atgctcggac gctcagaccc agcagtggct
     2221 tcccctgccc cgcccataca ccgaagacgc ggcccggagc tacgccctgg aagttgcacc
     2281 cgcagctctg gccagcggcc gcgcgcttga gcgggcaatc gagctgaacg gagaattcgc
     2341 cggcgtcatc ggcctcaaga acaccgactg ggccgtggcc aagacagagg ctggctactg
     2401 gcttgggccc tgggcaaggg gacggggaat cgccgctcga gcccttgatg ccatcaccga
     2461 ctgggcactc gacagccagg gcatcgagcg cgttgaggtg ctcgttgcca ccggcaacgc
     2521 cgcctcattg gccactgccc gcaaggccaa gttcatagag gaggggaccc tgcgtcgtgc
     2581 tggctacacc cacgacggaa aggtcgactt ggtcatcctg agtcgtctgg cgcacgaccc
     2641 tagcccgcgg ctctaacgcc ctcaattgcc gcgcggtagc gccgatgagc ggacgccggt
     2701 ggttacctaa ccgactccca ccagtcaaag attcgttcaa ccacgttggt ggtcacgagc
     2761 caaggctcta gcccgaagtg actggtcagt tcgttgcgga ggaactcggc aatctcggac
     2821 cggttgtcac cgcgcaacag gtgcgagatc agcggttcac ggatgcagtc gtactcgtct
     2881 actggccatg agccctggtc cacatcggct agatcgaaca cacctatggg gtcccactcg
     2941 ttcaacagtt cgcgcagttc tcgctgcgcc cgggcgtgtt gctcaatccg cggccacacg
     3001 tcatcgatgt ccgtcatgcc cgcagtctgc cttgccccgc gtcactggaa tcctaggggg
     3061 tgccttggta cctgaagaaa cacactcttc tggctccctt ctgtgtcaag acgccttggg
     3121 caggtcggtc gtaggctttg tggtgatggg tccgggaagt gacttatccg aagtgctggt
     3181 gtggggatgt gagccggccg cgctggattt cagtgtcgct ccccggtg
//
LOCUS       DAOSUG010000369         3196 bp    DNA     linear   ENV 10-JUN-2024
DEFINITION  MAG TPA_asm: Micropruina sp. isolate AMR_MDS_2195
            NODE_12357_length_3196_cov_3.458771, whole genome shotgun sequence.
ACCESSION   DAOSUG010000369 DAOSUG010000000
VERSION     DAOSUG010000369.1
DBLINK      BioProject: PRJNA925477
            BioSample: SAMN32864262
            Sequence Read Archive: SRR4236673
KEYWORDS    WGS; ENV; Metagenome Assembled Genome; MAG; Third Party Data; TPA;
            TPA:assembly.
SOURCE      Micropruina sp. (wastewater metagenome)
  ORGANISM  Micropruina sp.
            Bacteria; Bacillati; Actinomycetota; Actinomycetes;
            Propionibacteriales; Nocardioidaceae; Micropruina.
REFERENCE   1  (bases 1 to 3196)
  AUTHORS   Abdulkadir,N., Saraiva,J.P., Zhang,J., Stolte,S., Gillor,O.,
            Harms,H. and Rocha,U.
  TITLE     Genome-centric analyses of 165 metagenomes show that mobile genetic
            elements are crucial for the transmission of antimicrobial
            resistance genes to pathogens in activated sludge and wastewater
  JOURNAL   Microbiol Spectr 12 (3), e0291823 (2024)
   PUBMED   38289113
REFERENCE   2  (bases 1 to 3196)
  AUTHORS   Abdulkadir,N.
  TITLE     Direct Submission
  JOURNAL   Submitted (24-MAR-2023) Environmental Microbiology, Helmholtz
            Center for Environmental Research -UFZ, Permoserstr 15, Leipzig
            04318, Germany
COMMENT     The annotation was added by the NCBI Prokaryotic Genome Annotation
            Pipeline (PGAP). Information about PGAP can be found here:
            https://www.ncbi.nlm.nih.gov/genome/annotation_prok/
            
            ##Genome-Assembly-Data-START##
            Assembly Method        :: SPAdes v. 3.11.1
            Genome Representation  :: Full
            Expected Final Version :: Yes
            Genome Coverage        :: Not Applicable
            Sequencing Technology  :: Illumina MiSeq
            ##Genome-Assembly-Data-END##
            
            ##Genome-Annotation-Data-START##
            Annotation Provider               :: NCBI
            Annotation Date                   :: 05/04/2023 04:57:10
            Annotation Pipeline               :: NCBI Prokaryotic Genome
                                                 Annotation Pipeline (PGAP)
            Annotation Method                 :: Best-placed reference protein
                                                 set; GeneMarkS-2+
            Annotation Software revision      :: 6.5
            Features Annotated                :: Gene; CDS; rRNA; tRNA; ncRNA
            Genes (total)                     :: 2,776
            CDSs (total)                      :: 2,750
            Genes (coding)                    :: 2,714
            CDSs (with protein)               :: 2,714
            Genes (RNA)                       :: 26
            tRNAs                             :: 24
            ncRNAs                            :: 2
            Pseudo Genes (total)              :: 36
            CDSs (without protein)            :: 36
            Pseudo Genes (ambiguous residues) :: 0 of 36
            Pseudo Genes (frameshifted)       :: 2 of 36
            Pseudo Genes (incomplete)         :: 32 of 36
            Pseudo Genes (internal stop)      :: 2 of 36
            ##Genome-Annotation-Data-END##
FEATURES             Location/Qualifiers
     source          1..3196
                     /organism="Micropruina sp."
                     /mol_type="genomic DNA"
                     /submitter_seqid="NODE_12357_length_3196_cov_3.458771"
                     /isolate="AMR_MDS_2195"
                     /isolation_source="Activated sludge of municipal
                     wastewater treatment plants"
                     /db_xref="taxon:2737536"
                     /environmental_sample
                     /geo_loc_name="Canada"
                     /lat_lon="49.25 N 123.1 W"
                     /collection_date="2015-06-16"
                     /metagenome_source="wastewater metagenome"
                     /note="metagenomic"
     gene            complement(79..897)
                     /locus_tag="PLF56_11190"
     CDS             complement(79..897)
                     /locus_tag="PLF56_11190"
                     /inference="COORDINATES: protein motif:HMM:NF025887.2"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="nuclear transport factor 2 family protein"
                     /protein_id="HOQ54163.1"
                     /translation="MSADAPGSGPVGQVSADAPASGPVGRVSAAAPDSGPVGRVSAAA
                     PGVDLSADDELAPATYPHLGFEIGGDGDFEPGAYPDASIELEPGHHDESIELEPGAYP
                     DGSIELEPGANRGTLDAPTPGTGQARTKRSPAKDRAPTSTLNDGYSDDLFSLGEAQPS
                     DIEHVVALERSLLSDAVRADPAAVAALLHPDWYEIGRSGRLWSRPDQLAVIGVLPEPV
                     TLEVVLVDRLGPETILLLWRAQGADGSTLRTSLWVRSGGDWVQRFHQGTPESQL"
     gene            complement(925..1413)
                     /locus_tag="PLF56_11195"
                     /pseudo
     CDS             complement(925..1413)
                     /locus_tag="PLF56_11195"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_013862916.1"
                     /note="incomplete; partial in the middle of a contig;
                     missing C-terminus; Derived by automated computational
                     analysis using gene prediction method: Protein Homology.
                     GO_function: GO:0003676 - nucleic acid binding [Evidence
                     IEA];
                     GO_function: GO:0004523 - RNA-DNA hybrid ribonuclease
                     activity [Evidence IEA]"
                     /pseudo
                     /codon_start=1
                     /transl_table=11
                     /product="ribonuclease H"
     gene            1481..1654
                     /locus_tag="PLF56_11200"
     CDS             1481..1654
                     /locus_tag="PLF56_11200"
                     /inference="COORDINATES: ab initio
                     prediction:GeneMarkS-2+"
                     /note="Derived by automated computational analysis using
                     gene prediction method: GeneMarkS-2+."
                     /codon_start=1
                     /transl_table=11
                     /product="hypothetical protein"
                     /protein_id="HOQ54164.1"
                     /translation="MMQITVEGTEVCRVELVKAPGAATLGDGAAVTTAGTAATFYLAP
                     CRHRGPTDGMPQA"
     gene            complement(2021..>3196)
                     /locus_tag="PLF56_11205"
     CDS             complement(2021..>3196)
                     /locus_tag="PLF56_11205"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_013862504.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="helicase-related protein"
                     /protein_id="HOQ54165.1"
                     /translation="CALLAPTEVLAQQHYATIRALMGDLAGGRLLGSPEVATDVVLVT
                     GSLPAAAKREALLRAASGEAGIVIGTHALLHERVQFADLGFIVIDEQHRFGVEQRGVL
                     SDKASARPHVLVLTATPIPRSVAMTIFGDLDVSTLAELPAGRQVVGTVVVDAKRRPGW
                     VERAWQRVVEEVGQGRQVFVVVPRIKSSDEGMGVVDLAAELSAVHLAGLRVGVLHGQL
                     HSDVKEQTMATFAAGELDVLVATTVIEVGVDVPNATMMVIWDADRFGISQLHQLRGRI
                     GRGQHPGVCLLISSALDEPTIEDRLTAVAATNDGFALAELDLVQRREGDVLGAEQSGG
                     RSSLRLLSVLDHAEIIGIAKGIAAQAVERDPGCTTPGFQDAVTSIELLAAGDWLELS"
BASE COUNT          517 a         1113 c         1083 g          483 t
ORIGIN      
        1 aatgggtcag cccgttggtt gagcctgtcg aacccctcgt cgcacgaggt ctcgacaagc
       61 tgaccagcga tgggtcagct acagctgcga ctcgggcgtg ccctggtgga aacgctgaac
      121 ccagtcaccc ccggaccgca cccacagcga tgtccgcaac gtcgatccgt cggccccttg
      181 cgcacgccag agcagcagaa tcgtctcagg gccgagccgg tcgaccagaa ccacctccag
      241 cgtcaccggt tcaggcagca caccgatgac ggccaactgg tcgggcctgc tccacagccg
      301 cccggaccgt ccgatctcgt accagtccgg gtggagcaac gcggccaccg cggccgggtc
      361 ggcacgcacc gcgtcgctga gcagggagcg ctccagcgcg accacatgct cgatatcgga
      421 gggctgcgcc tcgccgagcg agaacaggtc gtcgctgtag ccatcgttga gcgtcgaggt
      481 gggggcgcgg tccttcgcag gtgaccgctt cgtccgagcc tggccggtgc cgggggtagg
      541 ggcgtcgagc gtgcctcggt tggcgcccgg ttcgagctcg atcgacccgt cggggtaagc
      601 gcccggttca agctcgatcg actcgtcgtg gtggcccggt tcgagctcga tcgacgcgtc
      661 ggggtaagcg cccggttcga aatcgccatc cccaccaatc tcaaaaccga ggtgaggata
      721 ggtggccggt gcgagctcgt cgtcggcact gaggtcgaca cccggcgcag cggcggacac
      781 ccgccccaca gggcctgaat ccggcgcagc ggcggacacc cgccccacag ggcccgaagc
      841 cggcgcgtcg gccgacacct gccccacagg gcccgaaccc ggagcgtcgg ccgacacccg
      901 ctcggcaggg gcggcgtccg tcgtgaaacc gggtccgttg ggtggcgtgc gtccttggct
      961 gaaggcgacg gccgctccgt tggccaaccg gtccgcctct tcgttgagcg gatgacccga
     1021 gtgccccttc acccactcga agcgcacccg ccggccttgc agagcctcgt ccagggcctt
     1081 catgatctcg acgttcagca caggtttgcc gtcgcccttg cgccagttct tgcgcttcca
     1141 gcccggcagc catttggtca gcgagttgat cacgtactgc gagtcgcagt agatcagcag
     1201 gtcgtcgtcg agtccggcgg tttgtcgaag caggtcgagt accgcggtca gttcgcccat
     1261 gttgttggtg ccgtgcggcc acccgccgct ggcccagtgg tcgggatcga tgtaccaggc
     1321 ccagcctgct gggcccggat tgcccagggc tgatccgtcg gcggcggcga tcaaagtcac
     1381 cgggtcatgg tgccagagcc gaacggaccc catcgcagcg gctcaatcat ggaatccagg
     1441 ccaccggcca gggcgggagg atgcggttga cacccccagc atgatgcaga tcaccgtgga
     1501 gggcaccgag gtctgcaggg tcgagctcgt caaggcgcca ggtgccgcaa cgctgggcga
     1561 tggtgcagcg gtcaccacgg ccggaactgc cgcgacgttc tacctcgccc cttgccgtca
     1621 ccgcgggccc acggatggca tgccccaggc ctgacattct gaaatgaccc gcaatagtcg
     1681 gggccggcct gcgcgtgtcg cccaccgacc tgccggctgg tgtcacgatc tcagaattcc
     1741 ttggcggcta cagaagcgag ggataccaga agtcaatggt gggcagcgca ccccaggcac
     1801 gctcgggacc ttcgcacgcg tccgcggcgc ggcatcgacc cgccaccgct tgctaccact
     1861 cagcccagat caccgatggg cgccgtcgcg agcggcacca gacgggtggc ttgacaagcc
     1921 ggtccgcgcg aaggcaaccg ggcctctggt gagcgtggcc gacgcagcca ggggtgcccg
     1981 gatggggccg tgcagtagtc gcagatcggt ggatctgcgc tcagctgagt tcgagccaat
     2041 cccccgccgc cagcagttcg atggaggtga cggcgtcctg aaagccgggc gtggtgcacc
     2101 ccggatcgcg ctcgaccgcc tgcgcagcga tgcctttggc gatgccgatg atctcggcgt
     2161 ggtcgagcac gctgagcagt cgcaggctgg atcgtccgcc ggactgttcg gcacccagca
     2221 cgtcgccctc gcggcgttga accaggtcga gttcggcgag cgcaaacccg tcgttggtgg
     2281 cggcgacggc ggtcagccgg tcctcgatcg tgggctcatc gagcgcggac gagatgagca
     2341 gacacacgcc gggatgctgg ccgcggccga tgcgtccgcg cagctgatgc agctgcgaga
     2401 tgccgaagcg atcggcgtcc cagatcacca tcatggtggc gttgggcacg tcgacgccga
     2461 cttcgatcac cgtggtggcg accagcacat cgagctcgcc ggcggcgaag gtggccatgg
     2521 tctgctcttt gacgtcgctg tgcagctggc cgtgcagcac gccgacccgc aacccggcca
     2581 ggtgcaccgc actcagttcg gcggccaggt caaccacccc catgccctcg tcgctggact
     2641 tgatgcgcgg caccacgacg aacacctgac ggccctgccc gacctcttcg accacccgct
     2701 gccacgcgcg ctccacccag cccggacggc gcttggcgtc gaccacgacg gtgcccacca
     2761 cctgccgtcc ggcgggcagt tcggcgagcg tggagacgtc gaggtcgccg aagatggtca
     2821 tggcaacgga gcgtggaatc ggcgtggcgg tgagcaccag aacgtgtgga cgagcggacg
     2881 ccttgtcgct gagcacgccc cgctgttcga cgccgaaccg gtgctgttcg tcgatcacga
     2941 tgaaacccag gtcggcgaac tgcacccgct cgtgcagcag ggcgtgcgtg ccgatcacga
     3001 tgcccgcctc accggacgcg gcgcgcagca acgcctcgcg cttggccgcc gcgggaagtg
     3061 agccggtcac cagcaccacg tcggtggcca cttctggtga gccgagcagc cgtccaccgg
     3121 ccaggtcgcc catcagcgcc cggatcgtgg cgtaatgctg ctgcgcgagc acctcggtgg
     3181 gcgccaacag cgcgca
//
LOCUS       DAOSUG010000370         3188 bp    DNA     linear   ENV 10-JUN-2024
DEFINITION  MAG TPA_asm: Micropruina sp. isolate AMR_MDS_2195
            NODE_12384_length_3188_cov_4.967124, whole genome shotgun sequence.
ACCESSION   DAOSUG010000370 DAOSUG010000000
VERSION     DAOSUG010000370.1
DBLINK      BioProject: PRJNA925477
            BioSample: SAMN32864262
            Sequence Read Archive: SRR4236673
KEYWORDS    WGS; ENV; Metagenome Assembled Genome; MAG; Third Party Data; TPA;
            TPA:assembly.
SOURCE      Micropruina sp. (wastewater metagenome)
  ORGANISM  Micropruina sp.
            Bacteria; Bacillati; Actinomycetota; Actinomycetes;
            Propionibacteriales; Nocardioidaceae; Micropruina.
REFERENCE   1  (bases 1 to 3188)
  AUTHORS   Abdulkadir,N., Saraiva,J.P., Zhang,J., Stolte,S., Gillor,O.,
            Harms,H. and Rocha,U.
  TITLE     Genome-centric analyses of 165 metagenomes show that mobile genetic
            elements are crucial for the transmission of antimicrobial
            resistance genes to pathogens in activated sludge and wastewater
  JOURNAL   Microbiol Spectr 12 (3), e0291823 (2024)
   PUBMED   38289113
REFERENCE   2  (bases 1 to 3188)
  AUTHORS   Abdulkadir,N.
  TITLE     Direct Submission
  JOURNAL   Submitted (24-MAR-2023) Environmental Microbiology, Helmholtz
            Center for Environmental Research -UFZ, Permoserstr 15, Leipzig
            04318, Germany
COMMENT     The annotation was added by the NCBI Prokaryotic Genome Annotation
            Pipeline (PGAP). Information about PGAP can be found here:
            https://www.ncbi.nlm.nih.gov/genome/annotation_prok/
            
            ##Genome-Assembly-Data-START##
            Assembly Method        :: SPAdes v. 3.11.1
            Genome Representation  :: Full
            Expected Final Version :: Yes
            Genome Coverage        :: Not Applicable
            Sequencing Technology  :: Illumina MiSeq
            ##Genome-Assembly-Data-END##
            
            ##Genome-Annotation-Data-START##
            Annotation Provider               :: NCBI
            Annotation Date                   :: 05/04/2023 04:57:10
            Annotation Pipeline               :: NCBI Prokaryotic Genome
                                                 Annotation Pipeline (PGAP)
            Annotation Method                 :: Best-placed reference protein
                                                 set; GeneMarkS-2+
            Annotation Software revision      :: 6.5
            Features Annotated                :: Gene; CDS; rRNA; tRNA; ncRNA
            Genes (total)                     :: 2,776
            CDSs (total)                      :: 2,750
            Genes (coding)                    :: 2,714
            CDSs (with protein)               :: 2,714
            Genes (RNA)                       :: 26
            tRNAs                             :: 24
            ncRNAs                            :: 2
            Pseudo Genes (total)              :: 36
            CDSs (without protein)            :: 36
            Pseudo Genes (ambiguous residues) :: 0 of 36
            Pseudo Genes (frameshifted)       :: 2 of 36
            Pseudo Genes (incomplete)         :: 32 of 36
            Pseudo Genes (internal stop)      :: 2 of 36
            ##Genome-Annotation-Data-END##
FEATURES             Location/Qualifiers
     source          1..3188
                     /organism="Micropruina sp."
                     /mol_type="genomic DNA"
                     /submitter_seqid="NODE_12384_length_3188_cov_4.967124"
                     /isolate="AMR_MDS_2195"
                     /isolation_source="Activated sludge of municipal
                     wastewater treatment plants"
                     /db_xref="taxon:2737536"
                     /environmental_sample
                     /geo_loc_name="Canada"
                     /lat_lon="49.25 N 123.1 W"
                     /collection_date="2015-06-16"
                     /metagenome_source="wastewater metagenome"
                     /note="metagenomic"
     gene            complement(<1..2263)
                     /locus_tag="PLF56_11210"
     CDS             complement(<1..2263)
                     /locus_tag="PLF56_11210"
                     /inference="COORDINATES: protein motif:HMM:NF013491.2"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_function: GO:0005524 - ATP binding [Evidence IEA];
                     GO_function: GO:0016301 - kinase activity [Evidence IEA];
                     GO_process: GO:0016310 - phosphorylation [Evidence IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="PEP/pyruvate-binding domain-containing protein"
                     /protein_id="HOQ54166.1"
                     /translation="MTRASTGITGLDDTIDDLRLGDNVVWQVDSEADFVAVAEPFVAR
                     ALADGRRVLYLSFGQRPPLLPDLSGIEVHTLDPFAGFERFAIAVHDLLAQVGRLGFYV
                     IDPLSDLHRAWRSDLMVANFFAATCPFLFELDTIAYFPLLRNRHSYATVATIRETTQL
                     LLDLHRIEDALYVQPLKVWQRHSPTMFFPHLLSGGRALSITSSAATTQLFASLARRVD
                     QPEVWQGVVDEAWAALEGGDEEQQTARQTLLEVLVGARGRMAELSRASLRLVDLLAVA
                     SRLIGTGRIGGKSLGMLVARAILEQCGAETLAGRLEPHDSFFIGSDVFVTFLVSNGWW
                     SHWTAHKQGADAPGGTIAAAERLHRFIPTGEFPRALREDFLQLLEHFGQAPIIVRSSS
                     LLEDDFGNAFAGKYASVFCVNQGTPTERLAALEDAIRSVYASAVGPEALQYRADRGLL
                     NLDEQMAILVQRVSGDLHDGLFFPLAAGVANSSSLYVWDPSLPDAGMARLVTGLGTRA
                     VDRTGRDSARIVSLADPSSSPGGDQEQGRWTQRRVDVLDLPADALRTVPIAEARAVVT
                     AADWALVARPDAAAARRLRELGRRGRVPEMVDFAALLDDAGVAELLRSMLATLAAAYR
                     HPVDVEFTVNLDPDGLAVLNLVQCRPLQTRGVGGSVGVPELVPERCLLSSRGNFMGGN
                     VRLPIDQVVWVRPDAYLRLGQPDRYAVAKRIGELNRALDGAAILIGPGRWGTSMPSLG
                     VPTHFTDLNGFVGL"
     gene            complement(2271..>3188)
                     /locus_tag="PLF56_11215"
     CDS             complement(2271..>3188)
                     /locus_tag="PLF56_11215"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_002516997.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_function: GO:0016491 - oxidoreductase activity
                     [Evidence IEA];
                     GO_process: GO:0006520 - amino acid metabolic process
                     [Evidence IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="Glu/Leu/Phe/Val dehydrogenase dimerization
                     domain-containing protein"
                     /protein_id="HOQ54167.1"
                     /translation="MRFCQSFMTELYRHLGEYTDVPAGDIGVGAREIGYLFGQYKRIT
                     NRYESGVLTGKGVSWGGSRARTEATGYGTVHFAEAMLKVRGDDFENRTVTVSGSGNVA
                     IYAVEKAQALGARVIAVSDSSGYVVDEGGIDLEVLKDVKEIRRGRVGDYADAVGSRAR
                     VITDGSIWDVPCDIALPCATQNELDGAQAGTLVGNGVRLVAEGANMPCTPDAVAVLRE
                     AGVLFAPGKASNAGGVATSALEMQQNASRDSWDFETTAARLEEIMYGIHDRCLATAEE
                     FGSPGDYVVGANLAGYIKVADAMVAMGVI"
BASE COUNT          514 a         1129 c         1084 g          461 t
ORIGIN      
        1 acagcccgac gaagccgttc aggtcggtga agtgggtcgg tacgccgagc gagggcattg
       61 acgttcccca gcgtcccggt ccgatcagga tcgccgcacc atcaagcgcc cggttgagtt
      121 cgccgatccg cttggccact gcgtagcgat cgggttggcc cagccgcagg taggcgtccg
      181 gacgcaccca caccacctga tcgatcggca gccggacgtt gccacccatg aagttgcccc
      241 ggctgctcaa caggcaccgc tccggaacca gctcaggcac ccccaccgag ccgccaaccc
      301 cccgggtctg cagtggacgg cactgcacca ggttgagcac cgcgagcccg tcggggtcaa
      361 ggttgacggt gaactcgacg tccaccgggt gccgatacgc ggcggcaagg gtggccagca
      421 tggagcgcag cagctcggcc acgccggcgt catcgagcag cgccgcgaag tccaccatct
      481 cgggtacccg tccacgcctg cccagctcgc gcagccgacg ggcggctgcc gcgtcgggac
      541 gggccaccag tgcccagtca gccgcggtca cgactgcgcg ggcctcggcg atcggtaccg
      601 tccggagcgc gtccgcgggc aggtccagca cgtccacccg acgctgagtc caccggccct
      661 gctcctggtc gcccccgggc gaggacgacg gatcggcgag gctgacgatc cgggcgctgt
      721 cccgtccggt gcgatcgacc gcgcgggtgc cgagcccggt gaccagccgg gccatcccgg
      781 cgtccggcag tgaggggtcc cagacataca gcgaggacga gttggccacc ccggcggcga
      841 gcggaaagaa caggccgtcg tgcaggtcgc cgctgacgcg ctgcaccagg atggccatct
      901 gttcgtcgag gttgagcagc ccacgatcgg cgcggtactg cagcgcctcc ggcccgacgg
      961 cgctggcgta gacggagcgg atcgcgtcct ccagtgctgc gagccgctcg gtcggggtgc
     1021 cctggttgac gcagaagacg ctggcgtact tgccggcgaa cgcgttgccg aagtcgtcct
     1081 ccagcagcga actcgaccgg acgatgatcg gcgcctggcc gaagtgttcg agcagttgca
     1141 gaaagtcttc gcgcagcgcc cgcggaaact cgccggtcgg gatgaaccgg tgtagtcgct
     1201 cggctgccgc gatggtgccg cccggagcgt cagccccctg cttgtgcgcc gtccagtgcg
     1261 accaccaccc gttgctgacc agaaaggtga cgaagacatc actgccgatg aagaaggagt
     1321 cgtgcggctc cagtcggccg gccagtgttt cggcgccgca ctgttccaga atcgcccggg
     1381 cgaccagcat gccgagactt ttgccgccga tccggccggt gccgatcagc ctggacgcga
     1441 ccgccagcag gtcgaccagg cgcaggctgg cgcgactcag ctccgccatc cggccgcggg
     1501 cgccgaccag cacctccagc agcgtctgcc gagcggtctg ctgctcctca tcgccaccct
     1561 ccagggcggc ccacgcctcg tccaccacgc cctgccagac ttcgggctgg tcgacccgcc
     1621 gggccagcga cgcgaacaac tgggtggtgg ccgccgacga cgtgatcgac agcgcccgac
     1681 caccgctgag cagatgcgga aagaacatcg tcggggagtg ccgctgccac accttgaggg
     1741 gctgcacgta cagggcgtcc tcgatgcggt gcaggtcgag cagcagctgg gtggtctcgc
     1801 ggatcgtcgc aacggtggcg tagctgtgcc ggtttcgcag cagcggaaag taggcgatgg
     1861 tgtcgagctc gaacagaaag gggcaggtgg cggcgaagaa gttcgccacc atcaggtcgg
     1921 agcgccaggc ccggtgcagg tcactgagcg ggtcgatcac atagaagccg agccggccca
     1981 cctgcgccag caggtcatgc acggcgatcg cgaaccgttc gaagccggcg aacgggtcga
     2041 gggtgtgcac ctcgatcccg ctcaggtcgg gcagcagcgg cggacgctgc ccgaagctga
     2101 gatagagcac ccggcgtccg tcggccaggg cgcgggccac gaacggctcg gcgacggcga
     2161 cgaagtcggc ctcggagtcg acctgccaca cgacgttgtc gccgagccgc aggtcgtcga
     2221 tggtgtcgtc caagccggtg atgccggtgc tggcccgcgt catcgtcggc tcagatcacg
     2281 cccatggcga ccatcgcgtc ggcgaccttg atgtagcccg ccaggttggc gccgaccacg
     2341 tagtcgccgg ggctgccgaa ctcctcggcg gtggccaggc aacggtcgtg gatgccgtac
     2401 atgatctctt cgagccgggc ggccgtggtc tcgaagtccc aggagtcgcg gctggcgttc
     2461 tgctgcatct cgagtgcgct ggtggccaca cccccggcat tggacgcctt gcccggtgcg
     2521 aacagcaccc cggcctcacg caacacggcg accgcgtccg gggtgcaggg catgttggca
     2581 ccctcggcga ccagccggac gccattgcct accaacgtgc ctgcctgcgc cccgtccagc
     2641 tcattctggg tggcgcaggg cagcgcgatg tcgcacggca cgtcccagat cgaaccgtcg
     2701 gtgatcacgc gggcgcgtga gccgaccgcg tccgcgtagt cgccgacccg gccgcgccgg
     2761 atctctttca cgtccttgag cacctcgagg tcgatgccgc cctcgtccac cacgtagccg
     2821 gacgaatcgg acaccgcgat cacccgcgcg cccagtgcct gggccttttc cacggcgtag
     2881 atcgccacgt tgcccgaacc cgaaacggtg accgtgcggt tctcgaagtc gtcgccgcgc
     2941 accttcagca tcgcctcggc gaaatggacg gtgccgtacc cggtcgcctc ggtacgcgcc
     3001 cgcgacccac cccagctgac gcctttgccg gtcagcacgc ccgactcgta gcggttggtg
     3061 atgcgcttgt actggccgaa caggtagccg atctcgcgcg cacccacgcc gatgtcaccg
     3121 gccggcacgt cggtgtactc gcccaggtgc cggtacagct cggtcatgaa cgactggcag
     3181 aaccgcat
//
LOCUS       DAOSUG010000371         3171 bp    DNA     linear   ENV 10-JUN-2024
DEFINITION  MAG TPA_asm: Micropruina sp. isolate AMR_MDS_2195
            NODE_12451_length_3171_cov_4.356547, whole genome shotgun sequence.
ACCESSION   DAOSUG010000371 DAOSUG010000000
VERSION     DAOSUG010000371.1
DBLINK      BioProject: PRJNA925477
            BioSample: SAMN32864262
            Sequence Read Archive: SRR4236673
KEYWORDS    WGS; ENV; Metagenome Assembled Genome; MAG; Third Party Data; TPA;
            TPA:assembly.
SOURCE      Micropruina sp. (wastewater metagenome)
  ORGANISM  Micropruina sp.
            Bacteria; Bacillati; Actinomycetota; Actinomycetes;
            Propionibacteriales; Nocardioidaceae; Micropruina.
REFERENCE   1  (bases 1 to 3171)
  AUTHORS   Abdulkadir,N., Saraiva,J.P., Zhang,J., Stolte,S., Gillor,O.,
            Harms,H. and Rocha,U.
  TITLE     Genome-centric analyses of 165 metagenomes show that mobile genetic
            elements are crucial for the transmission of antimicrobial
            resistance genes to pathogens in activated sludge and wastewater
  JOURNAL   Microbiol Spectr 12 (3), e0291823 (2024)
   PUBMED   38289113
REFERENCE   2  (bases 1 to 3171)
  AUTHORS   Abdulkadir,N.
  TITLE     Direct Submission
  JOURNAL   Submitted (24-MAR-2023) Environmental Microbiology, Helmholtz
            Center for Environmental Research -UFZ, Permoserstr 15, Leipzig
            04318, Germany
COMMENT     The annotation was added by the NCBI Prokaryotic Genome Annotation
            Pipeline (PGAP). Information about PGAP can be found here:
            https://www.ncbi.nlm.nih.gov/genome/annotation_prok/
            
            ##Genome-Assembly-Data-START##
            Assembly Method        :: SPAdes v. 3.11.1
            Genome Representation  :: Full
            Expected Final Version :: Yes
            Genome Coverage        :: Not Applicable
            Sequencing Technology  :: Illumina MiSeq
            ##Genome-Assembly-Data-END##
            
            ##Genome-Annotation-Data-START##
            Annotation Provider               :: NCBI
            Annotation Date                   :: 05/04/2023 04:57:10
            Annotation Pipeline               :: NCBI Prokaryotic Genome
                                                 Annotation Pipeline (PGAP)
            Annotation Method                 :: Best-placed reference protein
                                                 set; GeneMarkS-2+
            Annotation Software revision      :: 6.5
            Features Annotated                :: Gene; CDS; rRNA; tRNA; ncRNA
            Genes (total)                     :: 2,776
            CDSs (total)                      :: 2,750
            Genes (coding)                    :: 2,714
            CDSs (with protein)               :: 2,714
            Genes (RNA)                       :: 26
            tRNAs                             :: 24
            ncRNAs                            :: 2
            Pseudo Genes (total)              :: 36
            CDSs (without protein)            :: 36
            Pseudo Genes (ambiguous residues) :: 0 of 36
            Pseudo Genes (frameshifted)       :: 2 of 36
            Pseudo Genes (incomplete)         :: 32 of 36
            Pseudo Genes (internal stop)      :: 2 of 36
            ##Genome-Annotation-Data-END##
FEATURES             Location/Qualifiers
     source          1..3171
                     /organism="Micropruina sp."
                     /mol_type="genomic DNA"
                     /submitter_seqid="NODE_12451_length_3171_cov_4.356547"
                     /isolate="AMR_MDS_2195"
                     /isolation_source="Activated sludge of municipal
                     wastewater treatment plants"
                     /db_xref="taxon:2737536"
                     /environmental_sample
                     /geo_loc_name="Canada"
                     /lat_lon="49.25 N 123.1 W"
                     /collection_date="2015-06-16"
                     /metagenome_source="wastewater metagenome"
                     /note="metagenomic"
     gene            complement(<1..1195)
                     /gene="guaB"
                     /locus_tag="PLF56_11220"
     CDS             complement(<1..1195)
                     /gene="guaB"
                     /locus_tag="PLF56_11220"
                     /EC_number="1.1.1.205"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_014847133.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_function: GO:0003938 - IMP dehydrogenase activity
                     [Evidence IEA];
                     GO_process: GO:0009152 - purine ribonucleotide
                     biosynthetic process [Evidence IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="IMP dehydrogenase"
                     /protein_id="HOQ54168.1"
                     /translation="MSSSDLTPAGVPAPFATLGLTFDDVLLQPNASDVIPSQADASTQ
                     FSKRIRLNIPLVSAAMDTVTEARMAIAIAREGGMGILHRNMPADEQASQVDRVKRSEA
                     GMVDQPITITADRSIGEVDELCGKFRISGVPVVDDNFKLLGIVTNRDMRFEDDPNRRV
                     ADVMTPMPLVTGWPGISSDEALRLLARHKIEKLPLVDTDGTLRGLITLKDYVKSDKYP
                     LSSKDEQGRLRVGAAVGFFGDSWQRAMLLVEAGVDAIIVDTAHGHSTGVLDMIARLKA
                     EPAAAAVDVIGGNVATYAGAAALVDAGADGVKVGVGPGSICTTRVVAGVGVPQVTAIH
                     DVAQACRPAGVPVIGDGGLQYSGDIAKALVAGADTVMLGSLLAGCEESPGELVFINGK
                     QYKSYR"
     gene            complement(1559..2437)
                     /locus_tag="PLF56_11225"
     CDS             complement(1559..2437)
                     /locus_tag="PLF56_11225"
                     /inference="COORDINATES: protein motif:HMM:TIGR01777.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_function: GO:0003824 - catalytic activity [Evidence
                     IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="TIGR01777 family oxidoreductase"
                     /protein_id="HOQ54169.1"
                     /translation="MRIAIAGASGLLGSATAAHLRSTGHQVLALVRRPAQRLDERSWA
                     PDEGTITAPGLADVDAVINFSGAGVADERWTPQRKAELRSSRLNATTTIAAALDPNGR
                     CRRLLNGSAIGFYGDTGETVVDEGTPRGDGFLAQLVVDWEQAAHRAPVPTACLRTGQV
                     LTREGGFLGKQRLAFQLGLGGRIGTGRQFLSWIHIDDYTRAVAQLLDSTLTGPVNLVA
                     PHPVTNATFTEAFAQSLNRPALIPLPLPALRLLFGSELVDEALLSGTRVQPRRLLDAG
                     FAFDHPQLPEALAALR"
     gene            complement(2437..>3171)
                     /locus_tag="PLF56_11230"
     CDS             complement(2437..>3171)
                     /locus_tag="PLF56_11230"
                     /inference="COORDINATES: ab initio
                     prediction:GeneMarkS-2+"
                     /note="Derived by automated computational analysis using
                     gene prediction method: GeneMarkS-2+."
                     /codon_start=1
                     /transl_table=11
                     /product="hypothetical protein"
                     /protein_id="HOQ54170.1"
                     /translation="GVGVVALWLSLQRTPHVSASLVWAPILIGVAMALKQHAGLAVIA
                     AAGLPVAARLVRLPHLQRIAVLGWRTAVAAVVALVTFVVVSLATGLGFGWTLWLGEMG
                     RARTLTLSVAIGDAFAWTGHDLTGAAGTAIAVLALGGLAALLLLRPERPLEVTAWGSL
                     LVLGIGQALHPWYVGLSLALLALVPLRRTTARWVVFCTIGYLIAYCMNNLYNAAVLAS
                     IAVGVLVGTGAFALWVARGERPTRLG"
BASE COUNT          508 a         1123 c         1107 g          433 t
ORIGIN      
        1 cgcggtacga cttgtactgc ttgccgttga tgaacaccag ctcgccgggg ctctcttcgc
       61 acccggccag cagcgagccg agcatcaccg tgtcggcgcc ggccaccagc gccttggcga
      121 tgtcgccgga atactgcagg ccgccgtcgc cgatcaccgg cacccccgcc ggacgacagg
      181 cctgcgccac gtcgtggatc gcggtgacct ggggcacgcc cacgcccgcg accacccggg
      241 tggtgcagat cgagccgggc cccacgccca ccttgacgcc gtcggcaccc gcgtccacca
      301 gggccgcggc gcccgcgtac gtggccacgt tgccgccgat cacgtcgacc gcggcggccg
      361 ccggttcggc cttcaaccgg gcgatcatgt cgagcacgcc ggtggagtgg ccgtgtgcgg
      421 tgtcgacgat gatggcgtcc acaccggcct cgaccagcag catcgcgcgc tgccacgaat
      481 cgccgaagaa gccgaccgcg gcacccaccc gcagccggcc ctgctcgtcc ttggacgaca
      541 gcgggtactt gtcggacttc acgtagtcct tgagcgtgat caggccgcgc agcgtgccgt
      601 cggtgtcgac caggggcagc ttctcgatct tgtgccgggc cagcagccgc agggcctcgt
      661 cgctgctgat gccgggccag ccggtgacca gcggcatcgg ggtcatcaca tcggcgaccc
      721 ggcggttcgg atcgtcctcg aaccgcatgt cgcggttggt cacgatgccc agcagcttga
      781 agttgtcgtc caccaccggc acgcccgaga tgcggaactt gccgcacagt tcgtccacct
      841 cgccgatgct gcggtcggcg gtgatcgtga tcggctggtc gaccatgccc gcctcggagc
      901 gcttcacccg gtcgacctgc gaggcctgct cgtcggccgg catgttgcgg tgcagaatgc
      961 ccatgccgcc ctcgcgggcg atggcgatgg ccatgcgcgc ctcggtgacg gtgtccatgg
     1021 cggccgacac cagcggaatg ttgagccgga tgcgcttgga gaactgggtg gaggcgtccg
     1081 cctgcgaggg aatcacgtcg ctggcgttgg gctgcagcag cacgtcatcg aaggtcaggc
     1141 cgagcgtcgc gaacggcgcg ggaactccgg ccggggtcag atcggacgag gacatcgaag
     1201 catcactcct tcgcggcggg atggctcagt ctatgcgtcg cggacgggcc ggccctccgc
     1261 gtccagtggg aacccggagc atcgcggtcg acaggtccca aggccggcgc ctgaaatccc
     1321 tgcaccagtg ccaacaggcc cgcctgcaca cgtgtggacc cctcgcactg gtgcagggtt
     1381 ttcaggcgct gtgcggatag accctgaagg tggcggccga agggttgacg aggcaacgcg
     1441 tcttgaggag gtgctgatca atgcgcgctc cattggttga gcttgtcgag ccccctgacc
     1501 gcaggggtct acacgctcgg ccactaatcc tcgcgccctg ccggtgcgcc ccgaacggtc
     1561 agcgcaaggc cgccagggcc tcgggcagct gcgggtggtc gaaggcgaag cccgcatcga
     1621 gcagccggcg gggttgcacc cgggtgccgc tgagcagggc ctcgtcgacc aactcggaac
     1681 cgaagaggag ccgcaacgcg ggcagcggca gcggaatcag ggccgggcgg ttcagtgact
     1741 gggcgaatgc ctcggtgaac gtggcgttcg tgaccggatg cggagcgacc agattgaccg
     1801 gcccggtcag ggtcgagtcg agcaactgag cgacggcacg ggtgtagtcg tcgatgtgga
     1861 tccacgacag aaactgccgg ccagtaccga tccggccacc gagcccgagt tggaacgcca
     1921 gccgttgctt gcccaggaac ccgccctcgc gagtcagcac ctgaccggtg cgcaggcacg
     1981 ccgtcggcac cggagcccgg tgcgccgcct gctcccaatc gacgaccaat tgcgccagaa
     2041 acccgtcgcc tcgcggcgtg ccctcgtcga ccacggtttc accggtatcg ccgtagaagc
     2101 cgatcgccga gccgttcagc aggcggcggc agcgtccgtt cgggtcgagt gcggcggcga
     2161 tcgtggtggt cgcgttcaac cgggacgagc gcaactcggc cttgcgctgc ggcgtccagc
     2221 gctcatcggc gacgcctgcg ccggaaaagt tgatcaccgc gtccacatcg gcgagcccgg
     2281 gggcagtgat cgtgccctcg tcgggggccc aactgcgctc gtccagtcgc tgggccggcc
     2341 ggcgtaccag ggccagtacc tgatggccgg tgctgcgcag gtgggcggcg gtggcactgc
     2401 cgagcaggcc cgacgccccg gcgatcgcga ttcgcatcaa cccagcctag tcgggcgttc
     2461 tccccgggcg acccagagcg cgaaggcccc ggtgcccacc aggacgccga cggcgatcga
     2521 ggccagcacg gccgcgttgt agaggttgtt catgcagtag gcgatcagat agccgatggt
     2581 gcagaacacc acccaccgcg cggtcgtccg ccgcagcggc accagcgcca gcagcgcgag
     2641 gctcagcccg acataccagg ggtgcagcgc ctgcccgatg cccagcacca gcagcgatcc
     2701 ccaggccgtc acctcgagtg gacgctccgg ccgcagcagc agcagggcgg ccagtccgcc
     2761 caacgcgaga acggcgatcg cagtgccggc ggcaccggtc agatcgtggc ccgtccaggc
     2821 gaaggcgtca ccgatggcga cggacagggt gagcgtgcgc gcgcggccca tctcgccgag
     2881 ccagagcgtc cagccgaacc cgagcccggt ggccagcgag acgacgacga aggtgaccag
     2941 cgccaccacg gccgccacgg cggtccgcca accgagcacg gcgatgcgct gcaggtgcgg
     3001 caaccgcacc agccgggcgg ccaccggcag cccggccgcg gcgatcacgg ccagtccggc
     3061 atgctgcttg agcgccatgg cgaccccgat caggatcggc gcccagacca ggctcgctga
     3121 cacgtggggg gtccgttgca ggctcagcca cagcgcgacg accccgaccc c
//
LOCUS       DAOSUG010000372         3163 bp    DNA     linear   ENV 10-JUN-2024
DEFINITION  MAG TPA_asm: Micropruina sp. isolate AMR_MDS_2195
            NODE_12482_length_3163_cov_3.943694, whole genome shotgun sequence.
ACCESSION   DAOSUG010000372 DAOSUG010000000
VERSION     DAOSUG010000372.1
DBLINK      BioProject: PRJNA925477
            BioSample: SAMN32864262
            Sequence Read Archive: SRR4236673
KEYWORDS    WGS; ENV; Metagenome Assembled Genome; MAG; Third Party Data; TPA;
            TPA:assembly.
SOURCE      Micropruina sp. (wastewater metagenome)
  ORGANISM  Micropruina sp.
            Bacteria; Bacillati; Actinomycetota; Actinomycetes;
            Propionibacteriales; Nocardioidaceae; Micropruina.
REFERENCE   1  (bases 1 to 3163)
  AUTHORS   Abdulkadir,N., Saraiva,J.P., Zhang,J., Stolte,S., Gillor,O.,
            Harms,H. and Rocha,U.
  TITLE     Genome-centric analyses of 165 metagenomes show that mobile genetic
            elements are crucial for the transmission of antimicrobial
            resistance genes to pathogens in activated sludge and wastewater
  JOURNAL   Microbiol Spectr 12 (3), e0291823 (2024)
   PUBMED   38289113
REFERENCE   2  (bases 1 to 3163)
  AUTHORS   Abdulkadir,N.
  TITLE     Direct Submission
  JOURNAL   Submitted (24-MAR-2023) Environmental Microbiology, Helmholtz
            Center for Environmental Research -UFZ, Permoserstr 15, Leipzig
            04318, Germany
COMMENT     The annotation was added by the NCBI Prokaryotic Genome Annotation
            Pipeline (PGAP). Information about PGAP can be found here:
            https://www.ncbi.nlm.nih.gov/genome/annotation_prok/
            
            ##Genome-Assembly-Data-START##
            Assembly Method        :: SPAdes v. 3.11.1
            Genome Representation  :: Full
            Expected Final Version :: Yes
            Genome Coverage        :: Not Applicable
            Sequencing Technology  :: Illumina MiSeq
            ##Genome-Assembly-Data-END##
            
            ##Genome-Annotation-Data-START##
            Annotation Provider               :: NCBI
            Annotation Date                   :: 05/04/2023 04:57:10
            Annotation Pipeline               :: NCBI Prokaryotic Genome
                                                 Annotation Pipeline (PGAP)
            Annotation Method                 :: Best-placed reference protein
                                                 set; GeneMarkS-2+
            Annotation Software revision      :: 6.5
            Features Annotated                :: Gene; CDS; rRNA; tRNA; ncRNA
            Genes (total)                     :: 2,776
            CDSs (total)                      :: 2,750
            Genes (coding)                    :: 2,714
            CDSs (with protein)               :: 2,714
            Genes (RNA)                       :: 26
            tRNAs                             :: 24
            ncRNAs                            :: 2
            Pseudo Genes (total)              :: 36
            CDSs (without protein)            :: 36
            Pseudo Genes (ambiguous residues) :: 0 of 36
            Pseudo Genes (frameshifted)       :: 2 of 36
            Pseudo Genes (incomplete)         :: 32 of 36
            Pseudo Genes (internal stop)      :: 2 of 36
            ##Genome-Annotation-Data-END##
FEATURES             Location/Qualifiers
     source          1..3163
                     /organism="Micropruina sp."
                     /mol_type="genomic DNA"
                     /submitter_seqid="NODE_12482_length_3163_cov_3.943694"
                     /isolate="AMR_MDS_2195"
                     /isolation_source="Activated sludge of municipal
                     wastewater treatment plants"
                     /db_xref="taxon:2737536"
                     /environmental_sample
                     /geo_loc_name="Canada"
                     /lat_lon="49.25 N 123.1 W"
                     /collection_date="2015-06-16"
                     /metagenome_source="wastewater metagenome"
                     /note="metagenomic"
     gene            <1..1743
                     /locus_tag="PLF56_11235"
     CDS             <1..1743
                     /locus_tag="PLF56_11235"
                     /inference="COORDINATES: protein motif:HMM:NF012546.2"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_function: GO:0008236 - serine-type peptidase activity
                     [Evidence IEA];
                     GO_process: GO:0006508 - proteolysis [Evidence IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="prolyl oligopeptidase family serine peptidase"
                     /protein_id="HOQ54171.1"
                     /translation="RFADLRVVPSLRLVLAVREDHSAAGEPVNTLVALKLDGPNDDGG
                     AVLAGGADFYACLELSDGGRLAWMQWNHPAMPWDSCFVLTGQLDAESLAVAQVAVVAG
                     GAEESAVHPLWRGEELLFCSDRSGFWELYADGGRDSSEDEAARDPSAGRDPFVGRDPF
                     VGRDPSTGRYPSTGRNPSAGRACRDPRPLTALGADLVEPFWVFGRRPYALTGAGDVVI
                     RPRIDGRSQPMLVRAGEYLGLTTEVVDCDSLDACGDRIVLLAQFADVPTEIIELRPAH
                     RRAVRAAAPRVLAPEWVSHAEPVTWPGEAGEVHGWFYEPTNPLVEPGVAEVRPPVEPV
                     ETTDANAADGASTSTDGASARPPLIVISHGGPTAFSSSGFDLGIQFWTTRGFAVLDVN
                     YSGSSGYGRAYTDRLRGQWGVLDVADCIAGARSLADAGRVDGDRMVIRGGSAGGFTTL
                     RALTTSTVFAAGCSRYGIGDLAALATDTHKFESRYLDGLVGPWPAASEVYAERSPIHH
                     VDALNCPMLILQGEDDAVVPLNQARLMADAVRAKGLEAELIVFEGEGHGFRRAETIER
                     ALEAELAFYERVFG"
     gene            complement(1926..2789)
                     /locus_tag="PLF56_11240"
     CDS             complement(1926..2789)
                     /locus_tag="PLF56_11240"
                     /inference="COORDINATES: protein motif:HMM:NF013431.2"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="D-hexose-6-phosphate mutarotase"
                     /protein_id="HOQ54172.1"
                     /translation="MSQLSSEQPLPGIEAHPGSGHYGVFDHGAQVWAWQPDGAAHPVL
                     WLSSASAFEPGKPIRGGIPICFPWFGPGLNHDRTPPHGFVRTVSWARAKVHETHHALH
                     VDYEIDQTVTGDQRFFPAPYQARLSVTFAASHLEVSLRVENHDDEPFTFEEALHTYLA
                     VSDVRSVTIDGLDGARYLDKVAGDELFDNEQSGPLALTGPTDRVYMHSEEVSVDDPGF
                     QRRLVLSTSGSANVVVWNPWAEAAASIADIGEGEWTGFVCVEAANAYADAVTLLPGEH
                     WTMTQRIEVGS"
BASE COUNT          475 a         1015 c         1154 g          519 t
ORIGIN      
        1 cgattcgctg acctgcgcgt ggtgccgtcg ttaaggctgg tgctggccgt gcgggaggat
       61 cattccgccg ccggtgagcc ggtgaacacc ctggtggcgt tgaagctgga cgggcccaac
      121 gacgatggcg gcgcagtgct ggccggcggc gccgacttct acgcctgtct cgagttgagc
      181 gatgggggac ggctggcctg gatgcagtgg aaccacccgg cgatgccctg ggattcgtgc
      241 ttcgtgctga ccgggcagct ggacgctgag agcctggcgg tcgcgcaggt ggcagtggtg
      301 gcaggcggtg cggaggaatc tgccgtgcac ccgctgtggc ggggtgagga gttgctgttc
      361 tgttcggacc ggtcggggtt ctgggagctc tacgccgatg gtggtcgaga ttcatccgaa
      421 gacgaggcag cgcgagaccc atccgccggt cgggacccat tcgttggtcg cgacccattc
      481 gttggtcggg acccatccac cggtcgatac ccatccactg gtcggaaccc atccgctggt
      541 cgtgcttgtc gagacccacg cccactgacc gcactgggcg ccgatctggt ggagccgttc
      601 tgggtgttcg gacggcgtcc gtacgcactc accggtgccg gcgacgtggt gatccgcccg
      661 cgcatcgacg gccgcagcca accgatgcta gtgcgggcgg gggagtacct cgggctgacc
      721 accgaggtgg tcgactgcga cagcctggac gcgtgcggag accggatcgt gctgctggcc
      781 cagttcgcgg acgtaccgac cgagatcatc gaactgcgcc ccgcacatcg gcgggcggtg
      841 cgcgctgcag cgccccgggt gctggcgccc gagtgggtga gccacgccga gccggtgacc
      901 tggccgggag aggcgggcga ggtgcacggc tggttctacg agccgaccaa tccgctggtt
      961 gagcctggcg tggccgaggt ccgtccgccg gttgagcctg tcgaaaccac ggatgcgaac
     1021 gccgcggacg gggcctcgac ctcgaccgac ggggcctccg cacgtccgcc gttgatcgtg
     1081 atcagtcacg gcgggccgac cgcgttctcc tccagcggct tcgacctcgg cattcagttc
     1141 tggacgaccc ggggcttcgc cgtgctcgac gtcaactact ccggcagcag cggctacggg
     1201 cgggcgtaca ccgaccggct gcggggacag tggggagtgc tcgacgtggc cgactgcatc
     1261 gccggcgcgc gatcccttgc cgatgccgga cgggtggacg gtgaccgcat ggtgatccgc
     1321 ggcggcagcg ccggcgggtt tacgacgctg cgggcgctga ccacgtccac ggtgttcgcg
     1381 gccggctgca gccgctacgg catcggcgac ctggccgccc tggcgaccga cacgcacaag
     1441 ttcgagtcgc gctacctcga cggtctcgtc gggccctggc cggcggcgtc cgaggtgtac
     1501 gcggagcgct cgccgatcca ccacgtggac gctctcaact gcccgatgct gattttgcag
     1561 ggcgaggatg atgccgtggt gccgctcaac caggcgcggt tgatggccga cgccgtccgg
     1621 gccaagggcc tcgaggcgga attgatcgtg ttcgagggcg agggccacgg cttccgccgg
     1681 gcagagacca tcgagcgcgc actggaggcc gaactggcct tctacgagcg ggtgttcggg
     1741 tagggactcg gctggtcgcg ctcgtcgaaa cccctggcgc cgcatcggga cggctggtcg
     1801 gcccagagct ggaatgacgc ccggccgatc tcgacaggct cgatcaccga ggggttcccc
     1861 gggtcctgcg ctgaggcgcc cggccggatc cgacaggctc gatcaccgga tgcgggctca
     1921 gtcgctcagg accccacctc gatgcgttgc gtcatcgtcc aatgttcgcc gggcagcagc
     1981 gtgaccgcgt cggcatacgc gttggccgcc tcgacgcaga cgaagccggt ccactcaccc
     2041 tcgccgatgt cggcaatgct tgcagcagct tccgcccagg gattccacac caccacgttg
     2101 gctgatccgg acgtgctgag cacgagtcgc cgctgaaaac cgggatcgtc cacgctcacc
     2161 tcctcgctgt gcatgtagac gcggtcggtg gggccggtga gcgccagcgg cccgctctgc
     2221 tcgttgtcga agagttcgtc gccggccacc ttgtcgaggt agcgggcacc gtccagaccg
     2281 tcgatggtga cgctgcgcac gtcgctgacg gccaggtagg tgtgcagcgc ctcctcgaag
     2341 gtgaagggct cgtcgtcgtg gttttcgacc cgcagcgaca cctcgagatg gctcgccgca
     2401 aacgtgacgc tgagccgcgc ctgataggga gcggggaaga accgctggtc acccgtgacc
     2461 gtctggtcga tctcgtagtc gacgtgcagg gcatggtggg tttcgtgcac cttcgcccgc
     2521 gcccacgaca ccgtgcggac gaagccgtgc ggcggcgtcc gatcgtggtt gagaccggga
     2581 ccgaaccacg ggaagcagat cgggatgccg ccgcggatcg gcttaccggg ttcgaacgcg
     2641 ctggccgaac tgagccacag caccgggtgt gcggcgccgt ccggctgcca ggcccacacc
     2701 tgcgcgccgt ggtcgaagac gccgtagtga ccggatccgg ggtgggcttc gatgcccggc
     2761 aggggttgct cgctgctgag ttgactcatg aaaatggagc ctacggtgca tcaccgagca
     2821 ggggacaggt atgccacaag gggttcggtg cgtcgcggaa cagagcagat tcgcggaacg
     2881 gaacagcact gcggatccgt tgggtagcgg atccgcagcg ctggtgagaa gccgagggta
     2941 aggctcctgc aaggccgcgc gatgtcgtaa cgcgggggcg tccgtgatcg ggcggcctgg
     3001 tctggtcaag acactgcggg accgccgtaa cgatcccgca gtgccgggtg agagccgggg
     3061 ggatggctct cagttcatgg gttcagtacg cgccccgtga gccgagcacg gccccggcgg
     3121 tgcgcatcat gatggacagg tcctgcatca ccgaccagtt gtc
//
LOCUS       DAOSUG010000373         3153 bp    DNA     linear   ENV 10-JUN-2024
DEFINITION  MAG TPA_asm: Micropruina sp. isolate AMR_MDS_2195
            NODE_12526_length_3153_cov_4.024532, whole genome shotgun sequence.
ACCESSION   DAOSUG010000373 DAOSUG010000000
VERSION     DAOSUG010000373.1
DBLINK      BioProject: PRJNA925477
            BioSample: SAMN32864262
            Sequence Read Archive: SRR4236673
KEYWORDS    WGS; ENV; Metagenome Assembled Genome; MAG; Third Party Data; TPA;
            TPA:assembly.
SOURCE      Micropruina sp. (wastewater metagenome)
  ORGANISM  Micropruina sp.
            Bacteria; Bacillati; Actinomycetota; Actinomycetes;
            Propionibacteriales; Nocardioidaceae; Micropruina.
REFERENCE   1  (bases 1 to 3153)
  AUTHORS   Abdulkadir,N., Saraiva,J.P., Zhang,J., Stolte,S., Gillor,O.,
            Harms,H. and Rocha,U.
  TITLE     Genome-centric analyses of 165 metagenomes show that mobile genetic
            elements are crucial for the transmission of antimicrobial
            resistance genes to pathogens in activated sludge and wastewater
  JOURNAL   Microbiol Spectr 12 (3), e0291823 (2024)
   PUBMED   38289113
REFERENCE   2  (bases 1 to 3153)
  AUTHORS   Abdulkadir,N.
  TITLE     Direct Submission
  JOURNAL   Submitted (24-MAR-2023) Environmental Microbiology, Helmholtz
            Center for Environmental Research -UFZ, Permoserstr 15, Leipzig
            04318, Germany
COMMENT     The annotation was added by the NCBI Prokaryotic Genome Annotation
            Pipeline (PGAP). Information about PGAP can be found here:
            https://www.ncbi.nlm.nih.gov/genome/annotation_prok/
            
            ##Genome-Assembly-Data-START##
            Assembly Method        :: SPAdes v. 3.11.1
            Genome Representation  :: Full
            Expected Final Version :: Yes
            Genome Coverage        :: Not Applicable
            Sequencing Technology  :: Illumina MiSeq
            ##Genome-Assembly-Data-END##
            
            ##Genome-Annotation-Data-START##
            Annotation Provider               :: NCBI
            Annotation Date                   :: 05/04/2023 04:57:10
            Annotation Pipeline               :: NCBI Prokaryotic Genome
                                                 Annotation Pipeline (PGAP)
            Annotation Method                 :: Best-placed reference protein
                                                 set; GeneMarkS-2+
            Annotation Software revision      :: 6.5
            Features Annotated                :: Gene; CDS; rRNA; tRNA; ncRNA
            Genes (total)                     :: 2,776
            CDSs (total)                      :: 2,750
            Genes (coding)                    :: 2,714
            CDSs (with protein)               :: 2,714
            Genes (RNA)                       :: 26
            tRNAs                             :: 24
            ncRNAs                            :: 2
            Pseudo Genes (total)              :: 36
            CDSs (without protein)            :: 36
            Pseudo Genes (ambiguous residues) :: 0 of 36
            Pseudo Genes (frameshifted)       :: 2 of 36
            Pseudo Genes (incomplete)         :: 32 of 36
            Pseudo Genes (internal stop)      :: 2 of 36
            ##Genome-Annotation-Data-END##
FEATURES             Location/Qualifiers
     source          1..3153
                     /organism="Micropruina sp."
                     /mol_type="genomic DNA"
                     /submitter_seqid="NODE_12526_length_3153_cov_4.024532"
                     /isolate="AMR_MDS_2195"
                     /isolation_source="Activated sludge of municipal
                     wastewater treatment plants"
                     /db_xref="taxon:2737536"
                     /environmental_sample
                     /geo_loc_name="Canada"
                     /lat_lon="49.25 N 123.1 W"
                     /collection_date="2015-06-16"
                     /metagenome_source="wastewater metagenome"
                     /note="metagenomic"
     gene            <1..459
                     /locus_tag="PLF56_11245"
     CDS             <1..459
                     /locus_tag="PLF56_11245"
                     /inference="COORDINATES: ab initio
                     prediction:GeneMarkS-2+"
                     /note="Derived by automated computational analysis using
                     gene prediction method: GeneMarkS-2+."
                     /codon_start=1
                     /transl_table=11
                     /product="hypothetical protein"
                     /protein_id="HOQ54173.1"
                     /translation="QWRCLAWASGGSFFYGMSAGMVKATSEVMRHENFHGRPVFWVVA
                     PLLVLSYAVGGFMVQQALANGPAEVTVGSMTTTDPIVAVSFGLIVLGEGVHITPLVGV
                     GMLLAGALAVWGVVVLSRHHPDAARPVPAGHDTPARASVETQPLTGSAEI"
     gene            complement(429..1184)
                     /locus_tag="PLF56_11250"
     CDS             complement(429..1184)
                     /locus_tag="PLF56_11250"
                     /inference="COORDINATES: ab initio
                     prediction:GeneMarkS-2+"
                     /note="Derived by automated computational analysis using
                     gene prediction method: GeneMarkS-2+."
                     /codon_start=1
                     /transl_table=11
                     /product="hypothetical protein"
                     /protein_id="HOQ54174.1"
                     /translation="MTNTPGNGWPGGPPPQGPGSTPPGWGAQPWPPAAQSPAPQQYPP
                     QQYPPQQYPQQQYPGPPGATQPTGPQASRFDPTRALGEAPPQSVSINQFAPPPNRTPM
                     IATIVALVVLALVIGVGSYVRSQPAQPSASPSATPTASAEPGQPFSAPGGQQGRWQIL
                     DQKWTDEGLQLQLRIDADSAALSFSFIAFANASTTVVQPTASSASPDLRTGTAAPGRP
                     ATGYVFFPMPREDATIILATATGRQISALPVKG"
     gene            1281..2321
                     /locus_tag="PLF56_11255"
     CDS             1281..2321
                     /locus_tag="PLF56_11255"
                     /inference="COORDINATES: protein motif:HMM:NF025200.2"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="DUF4192 domain-containing protein"
                     /protein_id="HOQ54175.1"
                     /translation="MTADSIPTAHIRGIPDLLGVIPHLLGFHPTESVVFIVIDQTRVE
                     LTARADLAELQPAGHIELLVDRLLVRWPAARVWVVAYSRSEQAGWRVLRRAAAHLAHA
                     VAGEPICVSGRWYRVGDIDAPRHAHDPASSQSAASATVHGLQARPSRSVLRRSVQIDP
                     DQAYEAECAWLAATERLQNLASDARPAAMVTALRAGLSNPAQLTRDELVWLAALCLDP
                     DARDRALIAITHDNAEACVELWVLVVRACPMGLQGHALALVGWAGWVSGNGALQCVCL
                     EELDAIQASLPLQRMLNDLNQAIVPPSFWTELRAKLAAALTPLDESQMGADEAAGGIG
                     EPHGECADDQVA"
     gene            complement(2244..2819)
                     /locus_tag="PLF56_11260"
     CDS             complement(2244..2819)
                     /locus_tag="PLF56_11260"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_017934295.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_function: GO:0005384 - manganese ion transmembrane
                     transporter activity [Evidence IEA];
                     GO_process: GO:0030026 - cellular manganese ion
                     homeostasis [Evidence IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="VIT1/CCC1 transporter family protein"
                     /protein_id="HOQ54176.1"
                     /translation="MRSTSVTNARDAADVGDEPRGAALASRLNWLRAGVLGANDGIVS
                     TAGLVFGVAGATNDSVALFIAGVAGMVAGALSMAGGEYVSVSTQRDAENSIGVSVDEQ
                     TNPWHAALASLVSFTVGALVPLLAMVLTPVGTRLPVALAAVLLALLVTGYTSARLGSS
                     PVVRPILRNLIVGTLAMGLTYAAGSLVGAHL"
BASE COUNT          451 a         1044 c         1159 g          499 t
ORIGIN      
        1 cagtggcggt gcctggcgtg ggcctccggc ggttcgttct tctacggcat gtcggccggc
       61 atggtgaagg ccacctccga ggtgatgcgg cacgagaact ttcacggccg cccggtgttc
      121 tgggtggtgg cgccgctgtt ggtgctctcg tacgcagtgg gtggattcat ggtgcagcag
      181 gccctggcca acggcccggc cgaggtgacg gtgggatcga tgaccaccac cgacccgatc
      241 gtcgcggtca gtttcgggct gatcgtgctg ggtgagggtg tgcacatcac gccgctggtc
      301 ggggtgggca tgctgctcgc cggcgccctg gcggtgtggg gcgtggtcgt gctgtcgcga
      361 catcacccgg acgccgcccg tccggtacct gcggggcacg acacgcccgc gcgggcgagc
      421 gtcgagactc agcccttgac cggcagcgcc gagatctgac gccctgtggc cgtggccaga
      481 atgatcgtcg cgtcttcgcg cggcatcgga aagaacacgt agccggttgc gggtctgccc
      541 ggggccgcgg tgccggtgcg aaggtcgggc gaggctgaac tggcggtcgg ttgcacgacg
      601 gtcgttgacg cgttcgcaaa tgcgatgaag ctgaatgaga gcgccgccga gtcggcgtcg
      661 atgcgcagct gcaactgcag gccctcgtcg gtccacttct gatcgagaat ctgccagcgg
      721 ccctgttggc cgcccggtgc gctgaacggc tgccccggct cggccgaggc ggtcggggtc
      781 gcgctggggg acgcactggg ctgcgcgggc tgcgagcgaa cgtacgagcc caccccgatc
      841 accagcgcca gcacgaccag agccacgatg gtggcgatca tcggcgtgcg gttgggcggc
      901 ggtgcgaact ggttgatcga caccgactgc ggcggcgcct cgcccagcgc ccgggtcgga
      961 tcaaagcggg aggcttgggg gccggtcggc tgcgttgctc ccggggggcc cgggtactgc
     1021 tgttgggggt actgctgtgg ggggtactgc tgtggggggt actgctgcgg cgcgggggac
     1081 tgcgccgccg ggggccatgg ctgcgcgccc caaccgggcg gcgtggagcc gggcccctgg
     1141 ggcggcggcc cgccgggcca tccgttgccg ggtgtgttgg tcatcaccac gaaggttacc
     1201 cgtcggcggg ttgttcacac ccggccacgg atctcgagca ttttgaacac tggcggcggg
     1261 ctcggtcggt caatgatggt atgacggctg actcgatccc gaccgcccac atccggggca
     1321 ttccagacct gctcggggtg attcctcacc tgctgggctt tcatccgacc gaatcggtcg
     1381 tcttcatcgt gatcgatcaa accagggtcg agctgaccgc ccgggccgac ctcgccgagc
     1441 tgcagcccgc gggccacatc gagctgctgg tcgaccggct gctggtgcgc tggccggccg
     1501 cccgggtgtg ggtggtggcc tacagcaggt ccgagcaggc cggctggcgg gtgctgcgac
     1561 gcgctgcggc gcacctggcg catgcggtgg ccggcgaacc gatctgcgtc agcgggcggt
     1621 ggtaccgggt gggtgacatc gacgcgccgc gccacgctca cgatccggcc agcagccaga
     1681 gcgcagcctc ggccaccgtg cacgggctgc aggcacgccc cagccggtcg gtgctgcgcc
     1741 gcagcgtcca gatcgacccc gatcaagcct acgaggccga atgcgcatgg ctggccgcga
     1801 ccgagcgctt gcagaatctt gcctcggacg cccgtccggc agccatggtc acggcgctca
     1861 gggcgggctt gtcgaatccc gcccagctga cccgtgacga gctggtctgg ctggcggcgc
     1921 tgtgcctcga ccccgatgcg cgcgaccggg cgctgatcgc catcacccac gacaatgccg
     1981 aggcctgcgt cgagctctgg gtgctggtgg tgcgggcgtg tccgatgggg ctgcagggtc
     2041 acgcgctcgc gctggtgggc tgggccgggt gggtcagcgg caacggtgcc ttgcagtgcg
     2101 tctgcctcga agagctggac gcgatacagg cgtccctacc gcttcagcgc atgctgaacg
     2161 acctcaatca ggcgatagtg ccgcccagct tctggaccga actgcgcgcc aagctggccg
     2221 cggcgctgac cccgctggat gagtcacaga tgggcgccga cgaggctgcc ggcggcatag
     2281 gtgagcccca tggcgagtgt gccgacgatc aggttgcgta agatcggccg cacaacgggt
     2341 gagctgccca gccgggcgct ggtgtagccg gtcaccagca gggccagcag caccgctgcg
     2401 agagcgaccg gcagccgtgt acccaccggc gtcagcacca tggccagcag cggaacgagc
     2461 gctcccaccg tgaacgacac caacgaagcc agggcggcgt gccacgggtt ggtctgctcg
     2521 tcgacgctca cgccgatgga gttctcggcg tcgcgttgcg tgctgaccga cacgtactcg
     2581 ccgccggcca tggacaacgc gcccgccacc atgccggcca ctcccgcgat gaacagcgca
     2641 accgagtcgt tggtggcacc tgccaccccg aacaccaggc cggcggtcga cacgatgccg
     2701 tcgttggcgc ccagcacgcc ggcgcgcagc cagttcagtc gcgacgccag tgccgcgccc
     2761 cgaggctcgt cgcccacgtc ggcggcatcc cgggcgttgg tgacgctggt gcttcgcata
     2821 ccccaactgt gaactcgtgc acggtgggtc gcaagcaagg tgagcctgtc cttcgagcct
     2881 gcgcgcgtgg tcaacagcac gcctgtgaca aggtgagaca gcgacgaagc gggccttgaa
     2941 ggctcgatcg gcggtgctct tttccggtgc tgcgagccga ccgctggccc ggttccgcat
     3001 ggggtcgacg gcggtctttc gggtgaagtg cgccgaccgc tgacccggtt ctgcccaacg
     3061 gcgcgacgac tttgaacgat cgccgaacga cgggcacatg ctgaaggtat ggcagctcga
     3121 gcacgcctgg cagccgacga cgaactctcc gag
//
LOCUS       DAOSUG010000374         3151 bp    DNA     linear   ENV 10-JUN-2024
DEFINITION  MAG TPA_asm: Micropruina sp. isolate AMR_MDS_2195
            NODE_12535_length_3151_cov_3.083333, whole genome shotgun sequence.
ACCESSION   DAOSUG010000374 DAOSUG010000000
VERSION     DAOSUG010000374.1
DBLINK      BioProject: PRJNA925477
            BioSample: SAMN32864262
            Sequence Read Archive: SRR4236673
KEYWORDS    WGS; ENV; Metagenome Assembled Genome; MAG; Third Party Data; TPA;
            TPA:assembly.
SOURCE      Micropruina sp. (wastewater metagenome)
  ORGANISM  Micropruina sp.
            Bacteria; Bacillati; Actinomycetota; Actinomycetes;
            Propionibacteriales; Nocardioidaceae; Micropruina.
REFERENCE   1  (bases 1 to 3151)
  AUTHORS   Abdulkadir,N., Saraiva,J.P., Zhang,J., Stolte,S., Gillor,O.,
            Harms,H. and Rocha,U.
  TITLE     Genome-centric analyses of 165 metagenomes show that mobile genetic
            elements are crucial for the transmission of antimicrobial
            resistance genes to pathogens in activated sludge and wastewater
  JOURNAL   Microbiol Spectr 12 (3), e0291823 (2024)
   PUBMED   38289113
REFERENCE   2  (bases 1 to 3151)
  AUTHORS   Abdulkadir,N.
  TITLE     Direct Submission
  JOURNAL   Submitted (24-MAR-2023) Environmental Microbiology, Helmholtz
            Center for Environmental Research -UFZ, Permoserstr 15, Leipzig
            04318, Germany
COMMENT     The annotation was added by the NCBI Prokaryotic Genome Annotation
            Pipeline (PGAP). Information about PGAP can be found here:
            https://www.ncbi.nlm.nih.gov/genome/annotation_prok/
            
            ##Genome-Assembly-Data-START##
            Assembly Method        :: SPAdes v. 3.11.1
            Genome Representation  :: Full
            Expected Final Version :: Yes
            Genome Coverage        :: Not Applicable
            Sequencing Technology  :: Illumina MiSeq
            ##Genome-Assembly-Data-END##
            
            ##Genome-Annotation-Data-START##
            Annotation Provider               :: NCBI
            Annotation Date                   :: 05/04/2023 04:57:10
            Annotation Pipeline               :: NCBI Prokaryotic Genome
                                                 Annotation Pipeline (PGAP)
            Annotation Method                 :: Best-placed reference protein
                                                 set; GeneMarkS-2+
            Annotation Software revision      :: 6.5
            Features Annotated                :: Gene; CDS; rRNA; tRNA; ncRNA
            Genes (total)                     :: 2,776
            CDSs (total)                      :: 2,750
            Genes (coding)                    :: 2,714
            CDSs (with protein)               :: 2,714
            Genes (RNA)                       :: 26
            tRNAs                             :: 24
            ncRNAs                            :: 2
            Pseudo Genes (total)              :: 36
            CDSs (without protein)            :: 36
            Pseudo Genes (ambiguous residues) :: 0 of 36
            Pseudo Genes (frameshifted)       :: 2 of 36
            Pseudo Genes (incomplete)         :: 32 of 36
            Pseudo Genes (internal stop)      :: 2 of 36
            ##Genome-Annotation-Data-END##
FEATURES             Location/Qualifiers
     source          1..3151
                     /organism="Micropruina sp."
                     /mol_type="genomic DNA"
                     /submitter_seqid="NODE_12535_length_3151_cov_3.083333"
                     /isolate="AMR_MDS_2195"
                     /isolation_source="Activated sludge of municipal
                     wastewater treatment plants"
                     /db_xref="taxon:2737536"
                     /environmental_sample
                     /geo_loc_name="Canada"
                     /lat_lon="49.25 N 123.1 W"
                     /collection_date="2015-06-16"
                     /metagenome_source="wastewater metagenome"
                     /note="metagenomic"
     gene            <1..1272
                     /locus_tag="PLF56_11265"
     CDS             <1..1272
                     /locus_tag="PLF56_11265"
                     /inference="COORDINATES: protein motif:HMM:NF003716.0"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_component: GO:0016020 - membrane [Evidence IEA];
                     GO_function: GO:0008324 - monoatomic cation transmembrane
                     transporter activity [Evidence IEA];
                     GO_process: GO:0006813 - potassium ion transport [Evidence
                     IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="potassium/proton antiporter"
                     /protein_id="HOQ54177.1"
                     /translation="GFTTRWREIRPILGMASMLATIGICVSIVAMTWFGHVVLGLDLA
                     TSLLLGAITAPTDSAAVFSVLRRVPIPGRIRATLEAESGLNDAPVVLLVTAATAWALG
                     ETTGSPAVILGMVVLELLGGLAVGLVIGLIGARLLKRIALPASGLYPLGAMGWALLAD
                     GAGAFLHVSGFAAVYICAVVLGNSDLPHRNATKSFAEGVGWVAQIGLFVMLGLLAHPE
                     RFSTQVLVTGVLAGLFLTVIARPLSVWVCTVWFKVPWREQAFLSWAGLRGAVPIIMAT
                     VPLASALPQAKDLFDLVFVFVVVYTTLQAPTLGWVARTLGVTTGDDAVDIEVEVAPLD
                     KIQADFMQIKVPPGSLLHGVTVFELRLPPSTVVSLIIRNGTPFAPGAKDVIRSGDELL
                     VVVPSRFRYQVETRFKQIGRGGRLSKWHEER"
     gene            complement(1279..1842)
                     /locus_tag="PLF56_11270"
     CDS             complement(1279..1842)
                     /locus_tag="PLF56_11270"
                     /inference="COORDINATES: protein motif:HMM:NF024791.2"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="LytR C-terminal domain-containing protein"
                     /protein_id="HOQ54178.1"
                     /translation="MREVVRVIKTPVTLIVLLALVVFGGWWGLHNATAAIPPRPPQPC
                     VKIDVGGTLTPKYVTVRTLNAGLRGGLAKRVSTNLRSVGFYILKVNNSADRMAQTVIV
                     GNSKDSPEVKLVAGFFKNATRQGDGRVDHVVDVLLGDDFAGMATNPKTSIAVNGPVCL
                     PPLPAAEASALATPSTSPSPKPSPTKS"
     gene            complement(1891..2184)
                     /locus_tag="PLF56_11275"
     CDS             complement(1891..2184)
                     /locus_tag="PLF56_11275"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_013865904.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="type II toxin-antitoxin system VapB family
                     antitoxin"
                     /protein_id="HOQ54179.1"
                     /translation="MIFKRVGEERPYPEHGYVQRQWAAIAPQQIRLDQLVTTKRILDL
                     EQLLEDDSTFYGDLFAHVIFWHGDYYLEDGLHRALRAALQQRQTMHARVLELK"
     gene            2463..>3151
                     /locus_tag="PLF56_11280"
     CDS             2463..>3151
                     /locus_tag="PLF56_11280"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_020388606.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="DUF853 family protein"
                     /protein_id="HOQ54180.1"
                     /translation="MGNPDTAAIIKGYTFATDSIELGVLVADGNPVPDAKIRISLSML
                     NRHGLVAGATGTGKTKTLQLMAEQIARAGVSVFAADIKGDLSGLALPGTSSEKLLART
                     AAIGQQWQPHGCPVEFYALGGQGHGVPLRATISSFGPILLSKVLGLNETQESTLGLVF
                     HYCDTNGLPLLDLEDLKSVLSYLTAPEGKADLKELGGVSPATAGVILRSLVTLQTQGA
                     DEFFGEPEFDTR"
BASE COUNT          475 a         1018 c         1099 g          559 t
ORIGIN      
        1 ggcttcacca ccaggtggcg cgagattcgt ccgatactcg gcatggcgtc gatgctggcc
       61 acgatcggca tctgcgtatc gatcgtcgcg atgacctggt tcggccacgt cgtgctgggc
      121 ctcgacctgg ccacctcgct actgctgggc gcgatcaccg cacccaccga ttcggcggcg
      181 gtgttctcgg tgttgcgccg ggtgccgata ccgggccgga tccgcgccac cctcgaggcc
      241 gaatcgggcc tgaacgacgc accggtggtg ctgctggtca ccgccgccac ggcgtgggcg
      301 ttgggcgaga ccaccgggag cccggccgtg atcctgggca tggtggtgct cgaactgctc
      361 ggcggcctgg cggtcggcct ggtgatcggc ctgatcggcg cgcgactgct caaacgcatc
      421 gcgttgcccg cctcaggcct gtacccgctc ggggccatgg gctgggcgct gctggccgac
      481 ggtgccgggg cgttcctgca cgtgtcgggc tttgcggcgg tctacatctg tgccgtcgtt
      541 ctgggtaaca gtgacttgcc gcaccgcaac gccacgaagt cgttcgccga gggggtcggg
      601 tgggtcgcac agatcggcct gttcgtgatg ctcggcctgc tcgcccaccc ggaacggttc
      661 agcactcagg tgctggtcac cggcgtgctg gccggattgt tcctgaccgt gatcgcccgt
      721 ccgctgtcgg tatgggtgtg caccgtgtgg ttcaaggtgc cctggcgcga gcaggcgttc
      781 ctgtcgtggg cgggacttcg gggtgcggtt ccgatcatca tggcgacggt gccgctggcg
      841 tcggcgctgc cgcaggccaa ggacctgttc gacctggtct tcgtgttcgt cgtggtgtac
      901 accacgctgc aggccccgac cctgggctgg gtggcccgca cactgggcgt gacgacgggc
      961 gatgacgcgg tcgacatcga ggtcgaggtg gccccgctgg acaagatcca ggccgacttc
     1021 atgcagatca aggtgccgcc gggctcactg ctgcacggcg tgacggtgtt cgagttgcgg
     1081 ctaccgccga gcaccgtggt gtcgctgatc attcgtaacg gcaccccgtt cgcccccggc
     1141 gccaaagacg tgatcaggtc gggcgatgag ttgctggtcg tggtgccgtc gaggtttcgc
     1201 taccaggtcg agacccggtt caaacagatc ggacggggag gccggctgtc caagtggcac
     1261 gaggaacgct gagccgaact acgacttggt cggggacggc ttcggtgagg gcgaggtgga
     1321 cggggtggcg agcgcgctgg cctcggcggc cggcaacggc ggcaggcaga ccggaccgtt
     1381 caccgcgatc gaggtcttgg ggttcgtggc catgcctgca aaatcgtcgc cgagcagcac
     1441 gtccaccacg tggtcgacac ggccgtcgcc ctgccgggtg gcgttcttga agaagccggc
     1501 caccagcttg acctcggggc tgtctttgga attgccgacg atcacggtct gcgccatgcg
     1561 gtcggcgctg ttgttgacct tgagaatgta gaacccgacc gatcgcaggt tggtgctgac
     1621 gcgcttggcc aaaccgccgc gcaggccggc gttcagggtg cggacggtca cgtacttggg
     1681 ggtcagcgtg ccgcccacat cgatcttgac gcacggctgg ggcggacggg gcggtatggc
     1741 cgcggtggcg ttgtgaagac cccaccagcc gccgaacacg acgagcgcga gcagcacgat
     1801 cagcgtgacg ggtgtcttga tcacccgaac gacttcacgc aacgaactgc ccctctactc
     1861 gcctacacct ccaaccaggg tacttgagtt ctacttgagt tcgagcaccc gggcgtgcat
     1921 ggtctgtcgc tgctgcagcg cggcgcgcag cgcgcggtgc aggccgtcct cgaggtagta
     1981 gtcgccgtgc cagaagatga cgtgggcgaa caggtcgccg tagaaggtgg aatcgtcttc
     2041 gagcagttgc tccaggtcga gaatgcgttt ggtggtgacc agctggtcga gccggatctg
     2101 ctgcggcgcg atcgccgccc actgccgctg cacgtagccg tgctcggggt agggccgctc
     2161 ctcgccgaca cgcttgaaga tcacccggca gagtctaccg tccggtgagc gaccggccca
     2221 ctcatcaagc cgccacgtcc ggtactgaac ggagtgaacc gatgcgcggc agtcgatgac
     2281 cgcattcatc ggccgtccgg gatcgtctct ccttccaccc gttcatgatc atgtgtgcac
     2341 ggcgatcggt gcgcgtccgc caccccagcg ccgaaggtgt gaggatccaa ccaccccagg
     2401 gtcgaatcaa cacgcttcgc acggacgctc ccgctccgcc gtgcggccta tgatctggct
     2461 tcatgggtaa ccccgacacc gccgccatca tcaagggcta caccttcgcc accgactcga
     2521 tcgagttggg ggtgctggtc gccgacggca acccggtgcc cgacgcgaag attcgtatct
     2581 cgctgtcgat gctgaaccgg cacggcctgg tggccggcgc gaccggtacc ggcaagacca
     2641 agacgctgca gctgatggcc gagcagatcg cgcgcgcggg cgtctcggtg ttcgcggccg
     2701 acatcaaggg cgacctgtcg ggcctggcgc tgccgggcac atcgtccgag aagctgctgg
     2761 cgcgcaccgc cgcgatcggc cagcagtggc agccgcacgg ctgcccggtc gagttctacg
     2821 cgctgggtgg tcagggtcac ggggtgccgt tgcgagcgac gatcagttcg ttcgggccga
     2881 tcctgctcag caaggtgctc ggactcaacg aaacccagga gtcgacgctc ggcctggtgt
     2941 tccactactg cgacaccaac ggcctgccgc tgctcgacct cgaagacctc aagtcggtgc
     3001 tgtcgtacct gactgcgccc gagggcaagg ccgacctgaa agagctgggc ggggtgtcac
     3061 cggccaccgc gggcgtgatt ctgcgctcgc tggtcacgct gcagacgcag ggggcggacg
     3121 agttcttcgg cgagcccgag ttcgacaccc g
//
LOCUS       DAOSUG010000375         3149 bp    DNA     linear   ENV 10-JUN-2024
DEFINITION  MAG TPA_asm: Micropruina sp. isolate AMR_MDS_2195
            NODE_12541_length_3149_cov_3.948933, whole genome shotgun sequence.
ACCESSION   DAOSUG010000375 DAOSUG010000000
VERSION     DAOSUG010000375.1
DBLINK      BioProject: PRJNA925477
            BioSample: SAMN32864262
            Sequence Read Archive: SRR4236673
KEYWORDS    WGS; ENV; Metagenome Assembled Genome; MAG; Third Party Data; TPA;
            TPA:assembly.
SOURCE      Micropruina sp. (wastewater metagenome)
  ORGANISM  Micropruina sp.
            Bacteria; Bacillati; Actinomycetota; Actinomycetes;
            Propionibacteriales; Nocardioidaceae; Micropruina.
REFERENCE   1  (bases 1 to 3149)
  AUTHORS   Abdulkadir,N., Saraiva,J.P., Zhang,J., Stolte,S., Gillor,O.,
            Harms,H. and Rocha,U.
  TITLE     Genome-centric analyses of 165 metagenomes show that mobile genetic
            elements are crucial for the transmission of antimicrobial
            resistance genes to pathogens in activated sludge and wastewater
  JOURNAL   Microbiol Spectr 12 (3), e0291823 (2024)
   PUBMED   38289113
REFERENCE   2  (bases 1 to 3149)
  AUTHORS   Abdulkadir,N.
  TITLE     Direct Submission
  JOURNAL   Submitted (24-MAR-2023) Environmental Microbiology, Helmholtz
            Center for Environmental Research -UFZ, Permoserstr 15, Leipzig
            04318, Germany
COMMENT     The annotation was added by the NCBI Prokaryotic Genome Annotation
            Pipeline (PGAP). Information about PGAP can be found here:
            https://www.ncbi.nlm.nih.gov/genome/annotation_prok/
            
            ##Genome-Assembly-Data-START##
            Assembly Method        :: SPAdes v. 3.11.1
            Genome Representation  :: Full
            Expected Final Version :: Yes
            Genome Coverage        :: Not Applicable
            Sequencing Technology  :: Illumina MiSeq
            ##Genome-Assembly-Data-END##
            
            ##Genome-Annotation-Data-START##
            Annotation Provider               :: NCBI
            Annotation Date                   :: 05/04/2023 04:57:10
            Annotation Pipeline               :: NCBI Prokaryotic Genome
                                                 Annotation Pipeline (PGAP)
            Annotation Method                 :: Best-placed reference protein
                                                 set; GeneMarkS-2+
            Annotation Software revision      :: 6.5
            Features Annotated                :: Gene; CDS; rRNA; tRNA; ncRNA
            Genes (total)                     :: 2,776
            CDSs (total)                      :: 2,750
            Genes (coding)                    :: 2,714
            CDSs (with protein)               :: 2,714
            Genes (RNA)                       :: 26
            tRNAs                             :: 24
            ncRNAs                            :: 2
            Pseudo Genes (total)              :: 36
            CDSs (without protein)            :: 36
            Pseudo Genes (ambiguous residues) :: 0 of 36
            Pseudo Genes (frameshifted)       :: 2 of 36
            Pseudo Genes (incomplete)         :: 32 of 36
            Pseudo Genes (internal stop)      :: 2 of 36
            ##Genome-Annotation-Data-END##
FEATURES             Location/Qualifiers
     source          1..3149
                     /organism="Micropruina sp."
                     /mol_type="genomic DNA"
                     /submitter_seqid="NODE_12541_length_3149_cov_3.948933"
                     /isolate="AMR_MDS_2195"
                     /isolation_source="Activated sludge of municipal
                     wastewater treatment plants"
                     /db_xref="taxon:2737536"
                     /environmental_sample
                     /geo_loc_name="Canada"
                     /lat_lon="49.25 N 123.1 W"
                     /collection_date="2015-06-16"
                     /metagenome_source="wastewater metagenome"
                     /note="metagenomic"
     gene            complement(<1..1412)
                     /locus_tag="PLF56_11285"
     CDS             complement(<1..1412)
                     /locus_tag="PLF56_11285"
                     /inference="COORDINATES: protein motif:HMM:NF025620.2"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="carbohydrate-binding domain-containing protein"
                     /protein_id="HOQ54181.1"
                     /translation="MTFLANTKTWIAGGLAAVVLAVGGCTAATVAATTSTSSSTGTST
                     SATVALTADHDSTDDHTWDTSTEVTIDLSNPTATDGVSVKDGVITITAAGAYRLTGTL
                     ADGQVVVDTEDSGSVYLILDNASLTNHDGSAIVIDKADLALIVLTKGTTNTVTDGATY
                     AATGDDDPNSAIWSDPDLTILGEGTLNVTGNYNDAINGSDGVVIVSGTINVTAKDDGI
                     RGKDYLVIKGGTINVTSGSGDSLKSDNETDTGLGYVSLVDGTVTLKSGDDGIDAWNAI
                     SITGGTLTVAESVEGLEAKTISIAGGTTTVTASDDGINATDSASTSGDMQAQDGTVLN
                     ISGGELTVTADGDGLDSNGDITMSGGTVVVNGPTNSGNAALDYNGTFTITGGSLIAGG
                     SSGMAQAPSTDSSQKSLMFSVNAAAGSTLEVKDSSGTVIATYQATRQIGSLVVSNSKV
                     KEGETYTVYVDGTQSGTTDTS"
     gene            complement(1450..2049)
                     /locus_tag="PLF56_11290"
     CDS             complement(1450..2049)
                     /locus_tag="PLF56_11290"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_020391859.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="DUF4956 domain-containing protein"
                     /protein_id="HOQ54182.1"
                     /translation="MTLLSLGIDLAAILVLAFGIYFQRYRRREVMVAMIGLNVAVVAV
                     SAALASASVGLGLGLGLFGVLSIIRLRSSELSHSEVAYYFVSLAMGLLAGLAFDPTWL
                     GLLLIGLLVAVMYLADHPALFRGYRHQVLTLDRAITDEKELQTELAMLLGAQIKQFTV
                     QRVDQVRDTTVVDVRYRVRDSSPLARDTAANTADDALTH"
     gene            2196..>3149
                     /locus_tag="PLF56_11295"
     CDS             2196..>3149
                     /locus_tag="PLF56_11295"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_011757930.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_process: GO:0006629 - lipid metabolic process [Evidence
                     IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="patatin-like phospholipase family protein"
                     /protein_id="HOQ54183.1"
                     /translation="MSPRVALVLGSGGARGYAHIGAIQVLRERGYQIVGIAGTSMGAL
                     IGGLEAAGKLDDYTDWVTTLGPRDVMRLLDPSPFGAGAIKLERVLSRMTEILGGARIE
                     DLPIPYTAVATDLGARREVWFTAGPVDVAIRASVAIPGAIAPIMVNGRLLADGGLLNP
                     LPVDAVAAVPADFTLAISLSGWTPRDFNATPARATSEPRPPVEWLDQFRRTAAGIFEN
                     DWVASMLSRFGRAPDALDAPAPDALEPDPDQMLVAPAPAGLGIAEVAGLALDTMGGLI
                     TRFRLAAQPPDVLVTVPADAARTLDFHRAHELIELGRALTSD"
BASE COUNT          446 a         1032 c         1111 g          560 t
ORIGIN      
        1 gaggtgtccg tggtgcccga ctgggtgccg tccacgtaga cggtgtaggt ctcaccctct
       61 ttgaccttcg agttcgacac caccagcgac ccgatctgcc gcgtcgcctg gtacgtggcg
      121 atcaccgtgc cggaggagtc cttgacctcg agcgtcgatc cggccgccgc gttcacgctg
      181 aacatcaaag acttctgcga cgagtccgtg ctgggtgcct gcgccatgcc ggacgagccg
      241 ccggcgatca gtgagcctcc ggtgatggtg aaggtgccgt tgtagtcgag ggcggcattg
      301 ccgctgttgg tgggtccgtt caccaccacc gtgccgccgc tcatcgtgat gtcgccgttg
      361 gagtcgaggc cgtcgccgtc ggccgtcacg gtcaactcgc cgccggagat gttcagcacg
      421 gtgccgtcct gtgcctgcat gtcgccggac gtggacgccg agtcggtcgc gttgatgccg
      481 tcgtcgctgg ccgtcaccgt ggtggtgccg cccgcgatgc tgatcgtctt ggcctcgagc
      541 ccctccaccg actcggccac cgtcagggtg ccgccggtga tgctgatcgc gttccaggcg
      601 tcgatgccgt cgtcgcccga cttcagcgtc accgtgccgt ccacgaggct gacgtagcca
      661 aggccggtat cggtctcgtt gtcggacttg agcgagtcgc cgctgccgct ggtgacgttg
      721 atcgtgccgc ccttgatcac cagatagtcc ttgccgcgga tgccgtcgtc cttcgcggtg
      781 acgttgatcg tgcccgagac gatcacgact ccgtccgagc cgttgatggc gtcgttgtag
      841 ttgccggtca cgttcagcgt gccctcgccc agaatggtca ggtcgggatc gctccagatc
      901 gccgagttgg ggtcgtcgtc gccggtcgcg gcataggtgg cgccgtcggt gaccgtgttc
      961 gtggtgccct tggtgagcac gatcaacgcc aggtcggcct tgtcgatcac gatcgcagaa
     1021 ccgtcatggt tggtgaggga cgcgttgtcg agaatcaggt agacgctgcc cgaatcctcg
     1081 gtgtcgacca ccacctgacc gtcggccagg gtgccggtca accggtaggc gccggccgcg
     1141 gtgatggtga tcacgccgtc cttcacgctc accccgtcgg tcgcggtggg gttcgacagg
     1201 tcgatggtca cctctgtgct ggtgtcccag gtgtggtcgt cggtgctgtc gtgatcggcg
     1261 gtcaacgcca ccgtcgccga ggtggacgtg ccggtgctgg acgaggtgga ggtcgtcgcc
     1321 gccacggtcg cggctgtgca gccgcccacc gcgagcacca cggcggcgag gccgccggcg
     1381 atccaggtct tggtgttggc cagaaaggtc atgtcaggtt ctccgattcg agtgggtggg
     1441 tcggtggggt cagtgggtca gcgcgtcgtc ggcagtgttg gcggcggtat cgcgggccag
     1501 cggggaggaa tcgcgcaccc ggtagcgcac gtccaccacg gtggtgtcgc gcacctggtc
     1561 gacccgctgc acggtgaact gcttgatctg tgcgcccagc agcatcgcca gctcggtctg
     1621 cagctccttc tcgtcggtga tcgcccggtc gagggtgagc acctggtggc ggtagccgcg
     1681 gaacagggcg gggtggtcgg cgaggtacat cacggcgacc agcagcccga tcagcagcag
     1741 gccgagccag gtcgggtcga aggccaggcc ggcgagcagg cccatggcga gtgagacgaa
     1801 gtagtacgcc acttcggaat gactgagctc ggaggagcgc agccggatga tcgacagcac
     1861 gccgaacagc ccgagcccga gccccagccc gacggacgcg ctggcgagcg ccgcactgac
     1921 cgcgacgacg gccacgttga ggccgatcat ggccaccatc acctcgcggc ggcggtagcg
     1981 ctgaaagtag atgccgaagg ccaggacgag aatggcggcc aggtcgatgc cgaggctgag
     2041 cagagtcatg gggatttcct aagttcgttc gatcgtgatg attgaacgtt atggacgccg
     2101 tccgccgatc ggctttgagc ctgctgtgag ccatctgtga agcccggcgg gcttcacgag
     2161 cggtccgccg ggccggctga cctacgatga gccccatgag tccacgggtc gcgctggtgc
     2221 tgggatcggg gggtgcacgg ggctatgccc acatcggtgc gatccaggtg ttgcgcgaac
     2281 gcggctacca gattgtcggc atcgccggca cctcgatggg tgcgctgatc ggtggtctcg
     2341 aggcggccgg caagctggac gactacaccg actgggtcac gaccctcggg ccgcgcgacg
     2401 tgatgcggct gctcgatccc tccccgttcg gcgccggggc catcaagctg gagcgggtcc
     2461 tcagccgcat gacggagatt ctcggcgggg cccggatcga ggacctgccc atcccttaca
     2521 cggccgtcgc caccgacctg ggtgcccggc gcgaggtctg gttcaccgcc ggcccggtgg
     2581 acgtggcgat cagggcctcc gtggcgattc ccggtgcgat cgcaccgatc atggtcaacg
     2641 gacggctgct ggccgatggt ggcttgctga acccgttgcc ggtggacgcc gtggctgcgg
     2701 tgcccgccga tttcacgctg gccatctcgt tgtcgggttg gacgccacga gacttcaacg
     2761 ccactccggc cagggccacc tcggagcccc ggccacccgt ggaatggctg gaccagttcc
     2821 ggcgcaccgc cgccggcatc ttcgagaacg actgggtggc ctcgatgctg tcacggttcg
     2881 ggcgcgcgcc cgatgccctg gacgctcccg cgccggacgc cctcgagccg gacccggacc
     2941 agatgctggt cgctccggcg ccggccggac tgggcatcgc cgaggtcgcc ggcctggcgc
     3001 tcgacaccat gggcggcctg atcacccggt tccggttggc cgcccaacca ccggacgtgc
     3061 tcgtcaccgt gcccgccgat gccgcacgga cgcttgactt tcaccgcgcc cacgagctga
     3121 tcgaactggg ccgtgcgctg accagcgac
//
LOCUS       DAOSUG010000376         3141 bp    DNA     linear   ENV 10-JUN-2024
DEFINITION  MAG TPA_asm: Micropruina sp. isolate AMR_MDS_2195
            NODE_12589_length_3141_cov_4.328257, whole genome shotgun sequence.
ACCESSION   DAOSUG010000376 DAOSUG010000000
VERSION     DAOSUG010000376.1
DBLINK      BioProject: PRJNA925477
            BioSample: SAMN32864262
            Sequence Read Archive: SRR4236673
KEYWORDS    WGS; ENV; Metagenome Assembled Genome; MAG; Third Party Data; TPA;
            TPA:assembly.
SOURCE      Micropruina sp. (wastewater metagenome)
  ORGANISM  Micropruina sp.
            Bacteria; Bacillati; Actinomycetota; Actinomycetes;
            Propionibacteriales; Nocardioidaceae; Micropruina.
REFERENCE   1  (bases 1 to 3141)
  AUTHORS   Abdulkadir,N., Saraiva,J.P., Zhang,J., Stolte,S., Gillor,O.,
            Harms,H. and Rocha,U.
  TITLE     Genome-centric analyses of 165 metagenomes show that mobile genetic
            elements are crucial for the transmission of antimicrobial
            resistance genes to pathogens in activated sludge and wastewater
  JOURNAL   Microbiol Spectr 12 (3), e0291823 (2024)
   PUBMED   38289113
REFERENCE   2  (bases 1 to 3141)
  AUTHORS   Abdulkadir,N.
  TITLE     Direct Submission
  JOURNAL   Submitted (24-MAR-2023) Environmental Microbiology, Helmholtz
            Center for Environmental Research -UFZ, Permoserstr 15, Leipzig
            04318, Germany
COMMENT     The annotation was added by the NCBI Prokaryotic Genome Annotation
            Pipeline (PGAP). Information about PGAP can be found here:
            https://www.ncbi.nlm.nih.gov/genome/annotation_prok/
            
            ##Genome-Assembly-Data-START##
            Assembly Method        :: SPAdes v. 3.11.1
            Genome Representation  :: Full
            Expected Final Version :: Yes
            Genome Coverage        :: Not Applicable
            Sequencing Technology  :: Illumina MiSeq
            ##Genome-Assembly-Data-END##
            
            ##Genome-Annotation-Data-START##
            Annotation Provider               :: NCBI
            Annotation Date                   :: 05/04/2023 04:57:10
            Annotation Pipeline               :: NCBI Prokaryotic Genome
                                                 Annotation Pipeline (PGAP)
            Annotation Method                 :: Best-placed reference protein
                                                 set; GeneMarkS-2+
            Annotation Software revision      :: 6.5
            Features Annotated                :: Gene; CDS; rRNA; tRNA; ncRNA
            Genes (total)                     :: 2,776
            CDSs (total)                      :: 2,750
            Genes (coding)                    :: 2,714
            CDSs (with protein)               :: 2,714
            Genes (RNA)                       :: 26
            tRNAs                             :: 24
            ncRNAs                            :: 2
            Pseudo Genes (total)              :: 36
            CDSs (without protein)            :: 36
            Pseudo Genes (ambiguous residues) :: 0 of 36
            Pseudo Genes (frameshifted)       :: 2 of 36
            Pseudo Genes (incomplete)         :: 32 of 36
            Pseudo Genes (internal stop)      :: 2 of 36
            ##Genome-Annotation-Data-END##
FEATURES             Location/Qualifiers
     source          1..3141
                     /organism="Micropruina sp."
                     /mol_type="genomic DNA"
                     /submitter_seqid="NODE_12589_length_3141_cov_4.328257"
                     /isolate="AMR_MDS_2195"
                     /isolation_source="Activated sludge of municipal
                     wastewater treatment plants"
                     /db_xref="taxon:2737536"
                     /environmental_sample
                     /geo_loc_name="Canada"
                     /lat_lon="49.25 N 123.1 W"
                     /collection_date="2015-06-16"
                     /metagenome_source="wastewater metagenome"
                     /note="metagenomic"
     gene            complement(<1..740)
                     /locus_tag="PLF56_11300"
     CDS             complement(<1..740)
                     /locus_tag="PLF56_11300"
                     /inference="COORDINATES: protein motif:HMM:NF012798.2"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_function: GO:0003677 - DNA binding [Evidence IEA];
                     GO_process: GO:0006310 - DNA recombination [Evidence IEA];
                     GO_process: GO:0015074 - DNA integration [Evidence IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="tyrosine-type recombinase/integrase"
                     /protein_id="HOQ54184.1"
                     /translation="MQDELVAQWEHHLAVARNASPHTVRAYVGDLQNLTEYLQRTESG
                     ALSDVDLRTLRRWLAGQHAAGAERATLQRRAAAARVFFAWAHATGRLAHDPAIGLRSP
                     RVDRTLPPTLEVDQARAALDQLAGQAAALDQPGARAAALRDLAIVEVLYASGIRVGEL
                     CGLNLGSLDTDRQVVRVRGKGDKERTVPLGGPAVRALAAWRDQRHLVARAEAGDALFV
                     GDRGARIDQRVVRRLVHRTLARVPEAPDL"
     gene            875..1456
                     /gene="def"
                     /locus_tag="PLF56_11305"
     CDS             875..1456
                     /gene="def"
                     /locus_tag="PLF56_11305"
                     /EC_number="3.5.1.88"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_014846168.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_function: GO:0042586 - peptide deformylase activity
                     [Evidence IEA];
                     GO_process: GO:0036211 - protein modification process
                     [Evidence IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="peptide deformylase"
                     /protein_id="HOQ54185.1"
                     /translation="MSDDPTSGQVLPITRWGTPVMHSPTRPVTAFDDDLHTLVRDMVA
                     TMHAAQGVGLAATQVGLDLAVFVYECPDADDVVHRGVVCNPEVSVPESRDRALESADE
                     GCLSFPGAYQALARPERVVCTGQDADGNPVRIEGTGLLARCLQHETDHLNGTVFSDRL
                     STRARRLLTRQHDELAHLYPDDWPVTPRETSAG"
     gene            1483..>1953
                     /locus_tag="PLF56_11310"
     CDS             1483..>1953
                     /locus_tag="PLF56_11310"
                     /inference="COORDINATES: protein motif:HMM:NF022182.2"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="YbjN domain-containing protein"
                     /protein_id="HOQ54186.1"
                     /translation="MTMFNWGPTQPGGPPPFNRERWDGVLKNLEVNFGIDDDGDRFGD
                     WEFMRLWFLVEGANDDLMAIRSMWDVRPPVEAYDFIVEAVNTWNRDHFWPKTSVTRGE
                     DHLGVFGDLVIDVETGVTDEFLAQQVRCMIGTSNQLYTYLAEAFPESKDWFNAGA"
     assembly_gap    1954..2053
                     /estimated_length=100
                     /gap_type="within scaffold"
                     /linkage_evidence="paired-ends"
     gene            complement(2125..>3141)
                     /locus_tag="PLF56_11315"
     CDS             complement(2125..>3141)
                     /locus_tag="PLF56_11315"
                     /EC_number="3.1.1.103"
                     /inference="COORDINATES: protein motif:HMM:NF012371.2"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_function: GO:0016787 - hydrolase activity [Evidence
                     IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="serine hydrolase domain-containing protein"
                     /protein_id="HOQ54187.1"
                     /translation="DPILDAIVRTRVPDGEPGQVWAYNQVCTYLVAQAIAAVTGQPLT
                     SHVRRLLLDPLGGEGAKAQRTPFGRDLGFSGLHVTTPTIVALAQTWLDAGRWQGEQLV
                     PPPYVAAATVPTPASLRAEGMAGDWAHGYGFSFWGASHGYRGDGAFGQFALVLPEQQV
                     AVAITSEVEDMQATLDAVWTHLLPAVDAAVSSAADRQLAARLGELRHPPVRGGVDRTG
                     EVRAVRNAQSQLPDAFGAVRLRTTEAGHQVLVIEHAEGELITAVGNGEWLDSTWATRW
                     GPQLSIAASGAWHHGVFYAELRLVETPHTITVDLHPNHDVVLNWRLVPLTGSDPLNAV
                     GFPV"
BASE COUNT          470 a         1122 c         1009 g          440 t
ORIGIN      
        1 agatcggggg cctccggcac ccgggccagc gtgcgatgca ccagcctgcg caccacccgt
       61 tggtcgattc gggcgcctcg atcgccgacg aacagcgcgt caccggcctc ggcccgagcc
      121 accaggtgtc gctggtcacg ccaagcggcc aacgcgcgca ccgcgggtcc accgagcggc
      181 acggtgcgct ccttgtcgcc cttgccccgc acccgcacca cctggcggtc ggtgtcgagc
      241 gagccgaggt tcagcccgca caactccccc acgcgaatgc cgctggcgta cagcacctcg
      301 acgatcgcca ggtcacgcag tgcggccgcc cgagcgcccg gctggtcgag ggccgcggcc
      361 tgccccgcca gttggtcaag ggctgctctg gcctgatcca cctcgagcgt cggcggcagc
      421 gtgcggtcca cccgtggcga ccgcagcccg atggccgggt cgtgcgcgag ccgtccggtg
      481 gcatgcgccc aggcgaagaa cacccgggcg gccgctgcgc ggcgctgcag ggtcgcccgc
      541 tcagcgccgg cggcgtgctg accggccagc caacggcgca gggtgcgcag atcgacgtcg
      601 ctgagggctc cgctctcggt acgctgcaga tactcggtca ggttctgcag gtcgcccaca
      661 taggcccgca cggtgtgcgg cgaggcgttc cgggccacgg cgaggtgatg ctcccactgc
      721 gccaccagct cgtcctgcac gtgcctgagc ctacgatcca ggcgcagcga accgctgcgg
      781 cgacaccctc gcgggcgccg ccgcgtgcca tccacggcga tgagcgccgg cgatccatgg
      841 tcgctagcat ggacggccgt agagaaaggc ggccatgagc gacgatccga ccagcgggca
      901 ggttctgccg atcacccggt ggggcacccc ggtgatgcac agccccaccc gtccggtgac
      961 ggccttcgac gacgacctgc acactctggt gcgtgacatg gtcgccacca tgcacgccgc
     1021 ccagggcgtc gggctggctg ccacacaggt gggcctcgac ctcgccgttt tcgtctacga
     1081 gtgcccggac gccgacgatg tcgtgcatcg tggagtggtg tgcaatccag aggtatcggt
     1141 gcccgagagc cgtgaccgcg cgctcgagtc cgccgacgag ggctgcctgt cctttccggg
     1201 tgcgtaccag gccctcgccc gtcccgagcg ggtggtgtgc accggccagg acgccgacgg
     1261 caacccggtg cgcatcgaag gcaccggcct gctggcgcgc tgcctgcagc acgagaccga
     1321 tcatctcaac ggcacggtgt tcagcgaccg tctctcgacc cgggccaggc ggctgctgac
     1381 ccggcagcac gacgagttgg cccacttgta ccccgacgac tggccggtca cgccgcgaga
     1441 gacctcggcc ggctgaccgc ggaccgacga aaggaacatt cgatgaccat gttcaactgg
     1501 ggacccaccc agcccggtgg ccccccgccc ttcaaccgcg agcgctggga cggggtcctc
     1561 aagaacctcg aggtcaactt cggcatcgac gacgacggcg accggttcgg cgactgggag
     1621 ttcatgcggc tgtggttcct ggtggagggc gccaatgacg acctgatggc gatccggtcg
     1681 atgtgggacg ttcgtccccc cgtcgaggcg tacgacttca tcgtcgaagc ggtcaacacc
     1741 tggaaccgtg accatttctg gcccaagacc agcgtcaccc gtggtgagga ccatctcggc
     1801 gtgttcggtg acctggtgat cgacgtcgag accggcgtca ccgacgagtt cctggcccag
     1861 caggtgcgct gcatgatcgg cacctccaac cagctgtaca cctacctggc cgaggcgttt
     1921 cccgaatcga aggactggtt caacgccggc gcgnnnnnnn nnnnnnnnnn nnnnnnnnnn
     1981 nnnnnnnnnn nnnnnnnnnn nnnnnnnnnn nnnnnnnnnn nnnnnnnnnn nnnnnnnnnn
     2041 nnnnnnnnnn nnngcgttga gcttgtccag gctcggcact gaatccacgc tcagcgctga
     2101 gtccggctca gtcccgattc gggctcagac cgggaacccc accgcgttca gcgggtcgct
     2161 accggtcagc ggcaccagcc gccagttgag cacgacgtca tggttcggat gcaggtcgac
     2221 ggtgatcgtg tgcggcgtct cgaccagccg cagttcggcg tagaacacgc catggtgcca
     2281 cgccccactg gccgcgatgc tgagctgcgg cccccagcgc gtcgcccacg tcgagtcgag
     2341 ccattcgccg ttgccgaccg ccgtgatcag ttcgccctcg gcatgttcga tgaccagcac
     2401 ctggtgcccc gcttcggtgg tgcgcagccg gacggccccg aacgcgtccg gtagctgcga
     2461 ctgcgcattc cggacggccc tcacctcgcc cgtgcggtcc accccaccac gcaccggcgg
     2521 gtgtcgcagt tcacccagcc gcgccgccaa ctgccggtcg gcggccgacg acaccgcggc
     2581 atccaccgcc ggcagcagat gcgtccacac cgcgtcgagg gtggcctgca tgtcttcgac
     2641 ctcggacgtg atcgcgaccg ccacctgctg ctcgggcaac accagggcga actgcccgaa
     2701 cgcaccgtcg ccgcggtagc cgtgactcgc accccagaac gagaacccgt agccatgggc
     2761 ccaatcaccg gccattcctt cagcccgcag cgacgccggc gtcggcaccg tggcagccgc
     2821 cacgtacggc ggcggcacca actgctcacc ctgccagcgc ccggcatcca gccaggtctg
     2881 cgccaacgcc acaatggtcg gggtggtcac atgcagccca ctgaaaccga ggtcgcggcc
     2941 gaacggcgtg cgctgcgcct tggccccctc gccgcccaaa gggtcgagca gcaaccggcg
     3001 gacgtggctc gtcagcggct gcccggtgac cgccgcgatg gcctgcgcga cgaggtaggt
     3061 gcagacctgg ttgtaggccc agacctgtcc cggttcgccg tcgggcaccc gggtgcgcac
     3121 gatggcgtcc agaatcgggt c
//
LOCUS       DAOSUG010000377         3139 bp    DNA     linear   ENV 10-JUN-2024
DEFINITION  MAG TPA_asm: Micropruina sp. isolate AMR_MDS_2195
            NODE_12595_length_3139_cov_3.387808, whole genome shotgun sequence.
ACCESSION   DAOSUG010000377 DAOSUG010000000
VERSION     DAOSUG010000377.1
DBLINK      BioProject: PRJNA925477
            BioSample: SAMN32864262
            Sequence Read Archive: SRR4236673
KEYWORDS    WGS; ENV; Metagenome Assembled Genome; MAG; Third Party Data; TPA;
            TPA:assembly.
SOURCE      Micropruina sp. (wastewater metagenome)
  ORGANISM  Micropruina sp.
            Bacteria; Bacillati; Actinomycetota; Actinomycetes;
            Propionibacteriales; Nocardioidaceae; Micropruina.
REFERENCE   1  (bases 1 to 3139)
  AUTHORS   Abdulkadir,N., Saraiva,J.P., Zhang,J., Stolte,S., Gillor,O.,
            Harms,H. and Rocha,U.
  TITLE     Genome-centric analyses of 165 metagenomes show that mobile genetic
            elements are crucial for the transmission of antimicrobial
            resistance genes to pathogens in activated sludge and wastewater
  JOURNAL   Microbiol Spectr 12 (3), e0291823 (2024)
   PUBMED   38289113
REFERENCE   2  (bases 1 to 3139)
  AUTHORS   Abdulkadir,N.
  TITLE     Direct Submission
  JOURNAL   Submitted (24-MAR-2023) Environmental Microbiology, Helmholtz
            Center for Environmental Research -UFZ, Permoserstr 15, Leipzig
            04318, Germany
COMMENT     The annotation was added by the NCBI Prokaryotic Genome Annotation
            Pipeline (PGAP). Information about PGAP can be found here:
            https://www.ncbi.nlm.nih.gov/genome/annotation_prok/
            
            ##Genome-Assembly-Data-START##
            Assembly Method        :: SPAdes v. 3.11.1
            Genome Representation  :: Full
            Expected Final Version :: Yes
            Genome Coverage        :: Not Applicable
            Sequencing Technology  :: Illumina MiSeq
            ##Genome-Assembly-Data-END##
            
            ##Genome-Annotation-Data-START##
            Annotation Provider               :: NCBI
            Annotation Date                   :: 05/04/2023 04:57:10
            Annotation Pipeline               :: NCBI Prokaryotic Genome
                                                 Annotation Pipeline (PGAP)
            Annotation Method                 :: Best-placed reference protein
                                                 set; GeneMarkS-2+
            Annotation Software revision      :: 6.5
            Features Annotated                :: Gene; CDS; rRNA; tRNA; ncRNA
            Genes (total)                     :: 2,776
            CDSs (total)                      :: 2,750
            Genes (coding)                    :: 2,714
            CDSs (with protein)               :: 2,714
            Genes (RNA)                       :: 26
            tRNAs                             :: 24
            ncRNAs                            :: 2
            Pseudo Genes (total)              :: 36
            CDSs (without protein)            :: 36
            Pseudo Genes (ambiguous residues) :: 0 of 36
            Pseudo Genes (frameshifted)       :: 2 of 36
            Pseudo Genes (incomplete)         :: 32 of 36
            Pseudo Genes (internal stop)      :: 2 of 36
            ##Genome-Annotation-Data-END##
FEATURES             Location/Qualifiers
     source          1..3139
                     /organism="Micropruina sp."
                     /mol_type="genomic DNA"
                     /submitter_seqid="NODE_12595_length_3139_cov_3.387808"
                     /isolate="AMR_MDS_2195"
                     /isolation_source="Activated sludge of municipal
                     wastewater treatment plants"
                     /db_xref="taxon:2737536"
                     /environmental_sample
                     /geo_loc_name="Canada"
                     /lat_lon="49.25 N 123.1 W"
                     /collection_date="2015-06-16"
                     /metagenome_source="wastewater metagenome"
                     /note="metagenomic"
     gene            371..814
                     /locus_tag="PLF56_11320"
     CDS             371..814
                     /locus_tag="PLF56_11320"
                     /inference="COORDINATES: ab initio
                     prediction:GeneMarkS-2+"
                     /note="Derived by automated computational analysis using
                     gene prediction method: GeneMarkS-2+."
                     /codon_start=1
                     /transl_table=11
                     /product="hypothetical protein"
                     /protein_id="HOQ54188.1"
                     /translation="MTDSQHDDGYAWTWEPAVGALTTIALLAVVAVQAGRSLTLAAAG
                     AGWHWPPSAALVTSSWGILTGDLHAGLTTTHGAATVWVAWVIAGAVFIAGLAAAIVLA
                     LRVTSGRRFKGMATAGQAEQLLGLGRLRANRAVIRPDLYRKGHRR"
     gene            811..2244
                     /locus_tag="PLF56_11325"
     CDS             811..2244
                     /locus_tag="PLF56_11325"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_011755695.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="TraM recognition domain-containing protein"
                     /protein_id="HOQ54189.1"
                     /translation="MTRRFEPTEVGWRIGTSKVPVGVQLWQPWDRTAGVIGPQGSGKT
                     LDILTPALLQAPGAALVTLTKPDDLLLTLAARQACGPVAVLDPFGAAPGLPELIWDPI
                     DGCVDATVAEKRAKAFCAGTLGSGGDGADSAARFYAAEAAKVIQCYLHAAALAGYTLN
                     DVLEWSANPRTAALPSEILRSHPMAEPNWSGLLAGAVSNTDERTAGNTKSTVDQALAL
                     FATKDRRERCVPSDGRPAVDIADLIRRDGTIYLLGREDPYVSASPLMTAVAEHVLDTA
                     LQLANESGFGRLCPPMLACLDELPSTAPLPTLATRMANERALGISFIWASQTWAQLTR
                     IFGEQQARAIKDLTNSLIIFGGSKDAGFNREISDLLGTRRITRRNQQHGQHGSISTHG
                     EDTPVIRPEQLRELPTRTALVVAENGKPILATLHRAIDGKPGKRLLDQQHAARRLITN
                     TPDARLMSPGERTAMAHSYARQLGLTD"
     gene            2260..2820
                     /locus_tag="PLF56_11330"
     CDS             2260..2820
                     /locus_tag="PLF56_11330"
                     /inference="COORDINATES: ab initio
                     prediction:GeneMarkS-2+"
                     /note="Derived by automated computational analysis using
                     gene prediction method: GeneMarkS-2+."
                     /codon_start=1
                     /transl_table=11
                     /product="hypothetical protein"
                     /protein_id="HOQ54190.1"
                     /translation="MAQLLPFPDLTQRMLAAYIDLAAFARGGEAAEEIMATGEILPRI
                     WDLTTIADPDLRWDCWTWLDEFVVWLNAQHAWYSADHTPACWPEHPPLVRELGTLAFL
                     RFQAGEATGPLPLEEWHRYALPGYLDRTRDQRRACEEKHQPWPGRAAHTRHTNDDAAA
                     RRLRLFRDDCDLTSDDGEETVTLRAL"
     gene            complement(2850..>3139)
                     /locus_tag="PLF56_11335"
     CDS             complement(2850..>3139)
                     /locus_tag="PLF56_11335"
                     /inference="COORDINATES: ab initio
                     prediction:GeneMarkS-2+"
                     /note="Derived by automated computational analysis using
                     gene prediction method: GeneMarkS-2+."
                     /codon_start=3
                     /transl_table=11
                     /product="hypothetical protein"
                     /protein_id="HOQ54191.1"
                     /translation="RPPRSAAKTPPPSERSHTMTMKRSEFGSLGDRLGRMPRVLDDQR
                     VRHVWLDSTCPALVVDRRKTAGRWEGQVVRVEDGELLVGWVSGDRISTIEL"
BASE COUNT          533 a         1230 c          923 g          453 t
ORIGIN      
        1 acccgccacc gcctcgacac cggtactgtc gtcgacgtca ccctgatcct cgaccccgac
       61 agcacacaca gctggatcgc catcgccatc accacctgac aagcccaccc aaccaagctg
      121 cgggtggcgc gcctgcgctc gaacggcacc cgatggtggg taaccgcgtc ttgcggtgtc
      181 gtgtagtgtc cgggttctcc acccagagcg gtgtcgacat ccctcgacac catccagccg
      241 gaagcctcct gcgagccccc catcgccgga ggcttccgtg caccccagga caggaaccag
      301 ccacctgccc gatagctgat gtgctgaacg cttacgctgt gggcccgact cactcgggtt
      361 cttcaggggc atgacagatt cgcaacatga cgacggctac gcctggacct gggaacccgc
      421 cgtcggcgcc ctcaccacca tcgccctcct ggcagtggta gcggtccagg ccggccgcag
      481 cctcaccctc gccgccgccg gcgccggctg gcactggcca ccgtcggccg ccctggtcac
      541 cagcagctgg ggcatcctca ccggtgatct ccacgccggc ctcaccacca cccacggcgc
      601 cgccacagtc tgggtcgcgt gggtgattgc gggcgcggtg ttcattgccg gcctggccgc
      661 cgccatcgtc cttgcgctgc gtgtcacgtc aggccgtcgc ttcaagggca tggccacggc
      721 cggacaggct gaacagctcc tcgggctggg ccgtctccgc gctaaccggg ccgtgatccg
      781 ccccgacctg taccgaaagg gccaccggcg atgacgcgca ggtttgagcc gacggaggtg
      841 gggtggcgga tcgggaccag caaggtgccg gtgggcgtgc agttatggca gccgtgggac
      901 cgcaccgcgg gtgtgatcgg cccccaaggc tcgggcaaga ctctcgacat cctcaccccc
      961 gccctcctcc aggccccagg cgccgccctg gtcaccctca ccaaacccga cgacctcctc
     1021 ctcaccctcg ccgcacgcca agcttgcggc ccagtcgccg tgttggaccc gttcggtgcc
     1081 gcccctggcc tgccggagct gatctgggac ccgatcgacg gctgcgtcga cgccaccgtg
     1141 gcggagaagc gcgccaaggc gttctgcgcc ggcaccctcg gcagcggggg agacggggcc
     1201 gactccgccg cccgcttcta cgccgccgaa gccgcgaaag tcatccagtg ctacctccac
     1261 gccgcagccc tcgccggcta cacgttgaat gacgtcctcg aatggtccgc caacccccgc
     1321 accgccgccc tgccctcgga gatcctgcgg tcgcacccga tggcggaacc gaactggtcc
     1381 ggtctcctcg ccggcgcggt cagcaacacc gacgaacgca ccgccggcaa caccaaatcc
     1441 accgtcgacc aagcactggc cctcttcgcg acgaaggacc gacgcgaacg ctgcgtgccc
     1501 agcgacggcc gccccgccgt cgacatcgcc gacctcatcc gcagagacgg caccatctac
     1561 ctgctcggcc gcgaagaccc ctacgtgtcc gcctcaccgc tcatgacggc cgtcgccgag
     1621 cacgtcctcg acacggccct ccagctcgcg aacgagagcg ggttcggccg gctgtgtccg
     1681 ccgatgctgg cctgcctcga cgaactcccc tccaccgcac cactgccgac gctggcgacc
     1741 aggatggcga acgagcgggc gttggggatc tcgttcatct gggcctccca aacctgggcc
     1801 cagctgaccc gcatcttcgg cgagcagcag gcccgcgcga tcaaggacct caccaacagc
     1861 ctgatcatct tcggcggctc caaagacgcc gggttcaacc gcgagatcag cgacctcctc
     1921 ggcacccgcc ggatcacccg acggaatcaa cagcacggtc agcatggctc gatctccacc
     1981 cacggcgaag acacccccgt catccgaccc gaacaactcc gcgaactgcc cacccgcacc
     2041 gcgctggtgg tggcggagaa cgggaagccg atcctcgcca ccctccaccg cgccatcgac
     2101 ggcaaacccg gcaaacgcct cctcgaccag cagcacgcag cacgccggct catcaccaac
     2161 acccccgacg cccgcctgat gagcccgggg gagagaaccg ccatggcgca ctcctacgcc
     2221 cgccaactcg gcctcaccga ctgaaggacc tgaatcgcta tggctcagct actgccgttt
     2281 cccgatctca cacagcgcat gctcgccgcc tacatcgacc ttgcagcttt cgcccgcggg
     2341 ggagaggccg ctgaggagat catggccaca ggtgagatcc tgcccaggat ctgggacctc
     2401 accaccatcg ccgacccgga tctccgctgg gactgctgga cgtggctcga cgagttcgtc
     2461 gtctggctca acgcgcagca cgcctggtac tccgccgacc acacccccgc ctgctggccc
     2521 gagcacccac ccctggtccg ggaactcggc accctggcgt tcctaagatt ccaggccggc
     2581 gaagccaccg ggccgctgcc gttggaggag tggcaccgct acgccctgcc cggttacctc
     2641 gaccggacac gcgaccagcg ccgcgcctgc gaggagaagc accaaccctg gcccggccga
     2701 gccgcccaca cccgccacac caacgacgac gccgcagccc ggcgccttcg gctgtttcgg
     2761 gacgattgcg acctgacctc cgacgacggt gaagagacgg tcaccctcag ggccctctag
     2821 aggcgttcgg tcgcgctgag gggagagggc tatagctcga tggtggagat cctgtcgccg
     2881 ctgacccagc ccaccagtaa ctcaccgtcc tcgacccgca ccacctgccc ttcccagcgg
     2941 ccggcggtct tgcggcggtc aaccaccaag gcagggcagg tgctgtcgag ccaaacatga
     3001 cgcacccgct gatcgtcgag aacccttggc attcgtccga gacgatcgcc gagagaccca
     3061 aactcagatc gcttcatggt catggtgtgg cttcgttcgc ttggtggcgg ggtttttgca
     3121 gcgctccgtg gcggtcttg
//
LOCUS       DAOSUG010000378         3138 bp    DNA     linear   ENV 10-JUN-2024
DEFINITION  MAG TPA_asm: Micropruina sp. isolate AMR_MDS_2195
            NODE_12603_length_3138_cov_3.793383, whole genome shotgun sequence.
ACCESSION   DAOSUG010000378 DAOSUG010000000
VERSION     DAOSUG010000378.1
DBLINK      BioProject: PRJNA925477
            BioSample: SAMN32864262
            Sequence Read Archive: SRR4236673
KEYWORDS    WGS; ENV; Metagenome Assembled Genome; MAG; Third Party Data; TPA;
            TPA:assembly.
SOURCE      Micropruina sp. (wastewater metagenome)
  ORGANISM  Micropruina sp.
            Bacteria; Bacillati; Actinomycetota; Actinomycetes;
            Propionibacteriales; Nocardioidaceae; Micropruina.
REFERENCE   1  (bases 1 to 3138)
  AUTHORS   Abdulkadir,N., Saraiva,J.P., Zhang,J., Stolte,S., Gillor,O.,
            Harms,H. and Rocha,U.
  TITLE     Genome-centric analyses of 165 metagenomes show that mobile genetic
            elements are crucial for the transmission of antimicrobial
            resistance genes to pathogens in activated sludge and wastewater
  JOURNAL   Microbiol Spectr 12 (3), e0291823 (2024)
   PUBMED   38289113
REFERENCE   2  (bases 1 to 3138)
  AUTHORS   Abdulkadir,N.
  TITLE     Direct Submission
  JOURNAL   Submitted (24-MAR-2023) Environmental Microbiology, Helmholtz
            Center for Environmental Research -UFZ, Permoserstr 15, Leipzig
            04318, Germany
COMMENT     The annotation was added by the NCBI Prokaryotic Genome Annotation
            Pipeline (PGAP). Information about PGAP can be found here:
            https://www.ncbi.nlm.nih.gov/genome/annotation_prok/
            
            ##Genome-Assembly-Data-START##
            Assembly Method        :: SPAdes v. 3.11.1
            Genome Representation  :: Full
            Expected Final Version :: Yes
            Genome Coverage        :: Not Applicable
            Sequencing Technology  :: Illumina MiSeq
            ##Genome-Assembly-Data-END##
            
            ##Genome-Annotation-Data-START##
            Annotation Provider               :: NCBI
            Annotation Date                   :: 05/04/2023 04:57:10
            Annotation Pipeline               :: NCBI Prokaryotic Genome
                                                 Annotation Pipeline (PGAP)
            Annotation Method                 :: Best-placed reference protein
                                                 set; GeneMarkS-2+
            Annotation Software revision      :: 6.5
            Features Annotated                :: Gene; CDS; rRNA; tRNA; ncRNA
            Genes (total)                     :: 2,776
            CDSs (total)                      :: 2,750
            Genes (coding)                    :: 2,714
            CDSs (with protein)               :: 2,714
            Genes (RNA)                       :: 26
            tRNAs                             :: 24
            ncRNAs                            :: 2
            Pseudo Genes (total)              :: 36
            CDSs (without protein)            :: 36
            Pseudo Genes (ambiguous residues) :: 0 of 36
            Pseudo Genes (frameshifted)       :: 2 of 36
            Pseudo Genes (incomplete)         :: 32 of 36
            Pseudo Genes (internal stop)      :: 2 of 36
            ##Genome-Annotation-Data-END##
FEATURES             Location/Qualifiers
     source          1..3138
                     /organism="Micropruina sp."
                     /mol_type="genomic DNA"
                     /submitter_seqid="NODE_12603_length_3138_cov_3.793383"
                     /isolate="AMR_MDS_2195"
                     /isolation_source="Activated sludge of municipal
                     wastewater treatment plants"
                     /db_xref="taxon:2737536"
                     /environmental_sample
                     /geo_loc_name="Canada"
                     /lat_lon="49.25 N 123.1 W"
                     /collection_date="2015-06-16"
                     /metagenome_source="wastewater metagenome"
                     /note="metagenomic"
     gene            473..560
                     /locus_tag="PLF56_11340"
     tRNA            473..560
                     /locus_tag="PLF56_11340"
                     /product="tRNA-Ser"
                     /inference="COORDINATES: profile:tRNAscan-SE:2.0.12"
                     /note="Derived by automated computational analysis using
                     gene prediction method: tRNAscan-SE."
                     /anticodon=(pos:507..509,aa:Ser,seq:gga)
     gene            876..2576
                     /locus_tag="PLF56_11345"
     CDS             876..2576
                     /locus_tag="PLF56_11345"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_013860973.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_function: GO:0005524 - ATP binding [Evidence IEA];
                     GO_function: GO:0016887 - ATP hydrolysis activity
                     [Evidence IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="ATP-binding protein"
                     /protein_id="HOQ54192.1"
                     /translation="MTDSGSVGLVAGTSDSTPLRFHVAIGPHAYLQLDDIVTTERRIS
                     SRGVVRTAGVVTQVTARHEGASYGSDVFLISEGMLPAQVQELAEITTTRVEPECYVPP
                     LPGTSVRRATGEDRDHALYFDSMQKKMVVGLGRDGEPIYINLDFLDGTRGAHVSISGI
                     SGVATKTSFALFMLHSLFASEVLGARRTNAKALVFAVKGEDLLFLDKQNVRLDDDLRA
                     QYAQLGLPAHPFQSARFFAPPVRNDTTGRADVAGRTSGVHAFWWTLADFCAHELLPFV
                     FADAEDDRNQYTIVVHQVAARLAADVTSTDASGAVTIDGTTARTYADLVDLVVDRLQD
                     DNARPQWAGPATGVGTINAFIRRLRSSQRALSSLIRGDLPPSTTREVSTANSQVTVID
                     MHNLPERAQRFVVGVTLANETLRKERAGAGGLLFTMIDELNKYAPREGNSPIKDVLLD
                     IAERGRSLGIILIGAQQTASEVERRIVSNSSIRVVGRLDPAEAGRPEYGFLPPSQRQR
                     ATLAKPGAMFVSQPELPVPLAVEFPFPAWATRLSECAQEVTTSPVLARLANDDDLPPF
                     "
     gene            2587..>3138
                     /gene="sbcD"
                     /locus_tag="PLF56_11350"
     CDS             2587..>3138
                     /gene="sbcD"
                     /locus_tag="PLF56_11350"
                     /EC_number="3.1.11.-"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_013860972.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_function: GO:0004527 - exonuclease activity [Evidence
                     IEA];
                     GO_process: GO:0006260 - DNA replication [Evidence IEA];
                     GO_process: GO:0006310 - DNA recombination [Evidence IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="exonuclease subunit SbcD"
                     /protein_id="HOQ54193.1"
                     /translation="MKFLHTADWHVGKTLKGHSRLDEQRQVLAEMVGIAREHDVDAVL
                     ISGDLYDTSAPGSEAQKLVNHTLLTLAKEGREVVVIAGNHDHANTFEAFRPLMKAAGI
                     EFAGRVRATAHGGVHTFTAKSTGEQVVVVMLPFLSRRYAYGAEELVTGSPSENAGHYD
                     EAMRTVLHELCSAFQPEAVNLVMA"
BASE COUNT          543 a         1043 c          996 g          556 t
ORIGIN      
        1 aactcctttg attgtgtgtg ttcaagtcga cgctagcagt gcttctacgc ccgccaacga
       61 ctggtcagca ggtgctcgat ccgcgcctgc gtgcgcttcg ttcgcaggcc ggtggaacct
      121 gcgcgcgaag ccgtcgaggc ttgccgcggg tgcgacaggg gcacatggtg agcgattcga
      181 gaccgtggtc gtagtgggcc tccgcttgct gaggggaacc cggatgctgc caccgactct
      241 tgttgaccgt ctgtcgcgcg aggacgcacc ctatggcgac ctgacccggc ttcggaaatt
      301 tcgcggcctc gggcggcatc aacgacgccg cccgactacg cggccacgag ggtggacgct
      361 ctggccaggt cctggattta cgccggtggc gtcgccgaca tcgaggtgac catagaagcg
      421 ctgaacgacg ccagttggag ttcgcccgcc tacagccggt atcctgcttc gcggaggatt
      481 cgcctagagg cctatggcgc tcgcttggaa agcgggttgg gttcacaccc tcacgagttc
      541 gaatctcgta tcctccgcca gttgactagg ggaacgctgg cccgacgtca tcgtgtggtg
      601 gctcttggtt gccttctctt agttttctgg gctgatatga caagggcctc cgcatgtccc
      661 atgtcttgtc ctgccactgg agatcgcagc tgatccgtcg cgtggagcgt gccattttgc
      721 cgtcagccgc aactgctgct cggtcgactc gtctccttcg ccgtcgcatt gacggactgg
      781 taagtgatct caaccagctc aaccaaaact tcggcgggtc agaaatctgt cggggacgcc
      841 ccgataccgt gggccatgac gaaggagcga tcgtagtgac agattccggc tcggtaggtc
      901 tggtggcggg gacgagcgac tcaacaccgc tgcgatttca cgtagcaatt ggtccgcatg
      961 cctatctcca gttggacgac atcgtgacga ccgagagacg tatctcgtcg cgaggcgtcg
     1021 tccgcacggc cggagtcgtg actcaggtca cagcgcgcca cgagggggcc agctacgggt
     1081 ctgacgtctt tctgatctcc gaaggtatgc tgccggccca ggttcaggaa ctcgccgaga
     1141 tcaccacgac gcgcgtagag cccgaatgct atgttccgcc gctgccgggg acgtcggtgc
     1201 gccgagcgac gggcgaggac cgcgaccacg cgctctactt cgactcaatg cagaagaaga
     1261 tggtggtcgg gctaggccga gatggcgagc ccatctacat caacctcgac ttcctggacg
     1321 gcacgcgagg cgcccacgtc tcaatctcgg gcatctccgg cgtggccacc aagaccagct
     1381 tcgcgctgtt catgctgcac tccctcttcg cgtccgaggt gcttggcgcg cggcgcacca
     1441 acgcgaaggc gctggtgttc gcggtcaagg gcgaagacct gctgttcctc gacaagcaga
     1501 acgtgcggct cgatgacgat ctgcgcgccc agtacgcgca gttgggcctg cccgcgcacc
     1561 ccttccaatc agcgcgcttc ttcgcacccc cggttcgaaa cgacaccacc gggcgtgccg
     1621 acgtggcggg ccgcacgagc ggcgtccatg ccttctggtg gacactcgct gacttctgcg
     1681 cgcatgaact cctgcccttc gtcttcgccg acgctgagga cgaccgcaac cagtacacga
     1741 ttgtggtgca ccaggtcgca gcccggctcg ccgccgacgt gacgagcacg gacgcctcgg
     1801 gcgcggtcac catagacggc acgacggccc gcacctatgc cgatcttgtc gacctcgtgg
     1861 tcgaccggct gcaagatgac aacgcacgcc cccagtgggc gggaccggcg acaggggtgg
     1921 gtacgatcaa cgcgttcatc cgccgcctac gttcctcaca gcgggccctg tcctcgctca
     1981 tccggggtga tctacccccc agcacgacgc gcgaggtgtc gacggccaac agccaggtca
     2041 cggtgatcga catgcacaac ctccccgagc gtgctcagcg gttcgtggta ggcgtaacac
     2101 tcgctaacga gacgctccgc aaggagcggg ccggggcggg cgggctgctc ttcaccatga
     2161 tcgacgaact gaacaagtac gctccacgcg aaggcaactc cccgattaag gacgtgctgc
     2221 tcgacatcgc cgagcgaggc cgctcgctgg gcatcatctt gattggggcg cagcagacag
     2281 cctccgaggt ggagcgccgc atcgtctcga actcctctat ccgcgtggtt ggacgcctcg
     2341 acccagctga ggccggacgc cccgagtacg gcttcctgcc cccgagccag cgccagcgag
     2401 ccacgctggc aaagcccggc gccatgttcg tgtcgcagcc ggagttgcca gtgccgctcg
     2461 ctgtggagtt cccgttcccc gcgtgggcta cgcgactcag cgagtgtgcg caggaggtga
     2521 ccacgagtcc ggtgcttgcc aggctcgcca acgacgacga cctcccaccc ttttgaggag
     2581 actcacgtga agttcctgca caccgccgac tggcacgtcg gcaagacgct gaaaggccac
     2641 agtcgcctcg atgagcagcg ccaagttctg gccgagatgg tcggcatcgc acgggagcac
     2701 gacgttgacg cagtactcat ctccggcgac ctctacgaca cctcggctcc gggctcggag
     2761 gcgcaaaagc tggtcaacca cacgctgctg acgctggcga aggagggccg cgaggtggtc
     2821 gtcatcgctg gcaaccacga ccacgcgaac accttcgagg cgtttcgtcc gctcatgaag
     2881 gcggccggca tcgagttcgc cgggcgtgtc cgcgccacgg cccacggcgg cgtccacacg
     2941 ttcacggcga agtcgaccgg ggagcaggta gtggtcgtga tgctgccatt cctgtcgcgg
     3001 cgctacgcct atggcgccga ggagctggtg acgggctccc cgtccgagaa cgccggccac
     3061 tacgacgagg caatgcgcac cgtcctccac gaactctgct cggcattcca gccggaggcg
     3121 gtcaacctgg tcatggcc
//
LOCUS       DAOSUG010000379         3137 bp    DNA     linear   ENV 10-JUN-2024
DEFINITION  MAG TPA_asm: Micropruina sp. isolate AMR_MDS_2195
            NODE_12610_length_3137_cov_3.267683, whole genome shotgun sequence.
ACCESSION   DAOSUG010000379 DAOSUG010000000
VERSION     DAOSUG010000379.1
DBLINK      BioProject: PRJNA925477
            BioSample: SAMN32864262
            Sequence Read Archive: SRR4236673
KEYWORDS    WGS; ENV; Metagenome Assembled Genome; MAG; Third Party Data; TPA;
            TPA:assembly.
SOURCE      Micropruina sp. (wastewater metagenome)
  ORGANISM  Micropruina sp.
            Bacteria; Bacillati; Actinomycetota; Actinomycetes;
            Propionibacteriales; Nocardioidaceae; Micropruina.
REFERENCE   1  (bases 1 to 3137)
  AUTHORS   Abdulkadir,N., Saraiva,J.P., Zhang,J., Stolte,S., Gillor,O.,
            Harms,H. and Rocha,U.
  TITLE     Genome-centric analyses of 165 metagenomes show that mobile genetic
            elements are crucial for the transmission of antimicrobial
            resistance genes to pathogens in activated sludge and wastewater
  JOURNAL   Microbiol Spectr 12 (3), e0291823 (2024)
   PUBMED   38289113
REFERENCE   2  (bases 1 to 3137)
  AUTHORS   Abdulkadir,N.
  TITLE     Direct Submission
  JOURNAL   Submitted (24-MAR-2023) Environmental Microbiology, Helmholtz
            Center for Environmental Research -UFZ, Permoserstr 15, Leipzig
            04318, Germany
COMMENT     The annotation was added by the NCBI Prokaryotic Genome Annotation
            Pipeline (PGAP). Information about PGAP can be found here:
            https://www.ncbi.nlm.nih.gov/genome/annotation_prok/
            
            ##Genome-Assembly-Data-START##
            Assembly Method        :: SPAdes v. 3.11.1
            Genome Representation  :: Full
            Expected Final Version :: Yes
            Genome Coverage        :: Not Applicable
            Sequencing Technology  :: Illumina MiSeq
            ##Genome-Assembly-Data-END##
            
            ##Genome-Annotation-Data-START##
            Annotation Provider               :: NCBI
            Annotation Date                   :: 05/04/2023 04:57:10
            Annotation Pipeline               :: NCBI Prokaryotic Genome
                                                 Annotation Pipeline (PGAP)
            Annotation Method                 :: Best-placed reference protein
                                                 set; GeneMarkS-2+
            Annotation Software revision      :: 6.5
            Features Annotated                :: Gene; CDS; rRNA; tRNA; ncRNA
            Genes (total)                     :: 2,776
            CDSs (total)                      :: 2,750
            Genes (coding)                    :: 2,714
            CDSs (with protein)               :: 2,714
            Genes (RNA)                       :: 26
            tRNAs                             :: 24
            ncRNAs                            :: 2
            Pseudo Genes (total)              :: 36
            CDSs (without protein)            :: 36
            Pseudo Genes (ambiguous residues) :: 0 of 36
            Pseudo Genes (frameshifted)       :: 2 of 36
            Pseudo Genes (incomplete)         :: 32 of 36
            Pseudo Genes (internal stop)      :: 2 of 36
            ##Genome-Annotation-Data-END##
FEATURES             Location/Qualifiers
     source          1..3137
                     /organism="Micropruina sp."
                     /mol_type="genomic DNA"
                     /submitter_seqid="NODE_12610_length_3137_cov_3.267683"
                     /isolate="AMR_MDS_2195"
                     /isolation_source="Activated sludge of municipal
                     wastewater treatment plants"
                     /db_xref="taxon:2737536"
                     /environmental_sample
                     /geo_loc_name="Canada"
                     /lat_lon="49.25 N 123.1 W"
                     /collection_date="2015-06-16"
                     /metagenome_source="wastewater metagenome"
                     /note="metagenomic"
     gene            complement(<1..453)
                     /gene="gmk"
                     /locus_tag="PLF56_11355"
     CDS             complement(<1..453)
                     /gene="gmk"
                     /locus_tag="PLF56_11355"
                     /EC_number="2.7.4.8"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_016667666.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_function: GO:0004385 - guanylate kinase activity
                     [Evidence IEA];
                     GO_process: GO:0015949 - nucleobase-containing small
                     molecule interconversion [Evidence IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="guanylate kinase"
                     /protein_id="HOQ54194.1"
                     /translation="MSGDVTVISGPTAVGKGTVTKALKALHPELWVSVSATTRPPRPG
                     ERNGEHYWFIGDADFDELIATDGLLEWAVVHGRDRYGTLSAPVQQAVERGERVILEID
                     LQGARQVRQRLPQARLVFIAPPSWDELVNRLIGRGTETAEQRERRLVTA"
     gene            complement(443..760)
                     /gene="mihF"
                     /locus_tag="PLF56_11360"
     CDS             complement(443..760)
                     /gene="mihF"
                     /locus_tag="PLF56_11360"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_002517913.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="integration host factor, actinobacterial type"
                     /protein_id="HOQ54195.1"
                     /translation="MAIPQLTTEQLQAARAAATEARRSRAALKARVRSGELSLAQALD
                     EAADSEVLAHVKVVDLLKALPRVGDKRATLVMERYDIAANRRIRGLGRHQLAGLKAEF
                     GER"
     gene            complement(847..1674)
                     /gene="pyrF"
                     /locus_tag="PLF56_11365"
     CDS             complement(847..1674)
                     /gene="pyrF"
                     /locus_tag="PLF56_11365"
                     /EC_number="4.1.1.23"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_013863519.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_function: GO:0004590 - orotidine-5'-phosphate
                     decarboxylase activity [Evidence IEA];
                     GO_process: GO:0009220 - pyrimidine ribonucleotide
                     biosynthetic process [Evidence IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="orotidine-5'-phosphate decarboxylase"
                     /protein_id="HOQ54196.1"
                     /translation="MTTYRQRLARRTTERGNLCVGIDPHPGLLQAWGLADDPSGLEVF
                     ARRVVEVLGAKVAVFKPQSAFFERHGSAGIGVLERVLGDIAAAGALSILDVKRGDIGS
                     TMGAYTDAYLVDGAPLAADAITVSPYLGFGSLQPAFDAAERSGRGVYVLARTSNPEGH
                     LVQLALGEDHEGSVAQEIIDAARRANSASGQGAIGLVVGGTHDDLGCDVSGFNGSILV
                     PGIGFQGGRIDDLPALFGDAIDVVLPAVSRDVIAAGPDAAALNGRVDRLLGVADQTH"
     gene            complement(1718..2830)
                     /locus_tag="PLF56_11370"
     CDS             complement(1718..2830)
                     /locus_tag="PLF56_11370"
                     /EC_number="1.3.5.2"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_013161042.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_component: GO:0016020 - membrane [Evidence IEA];
                     GO_function: GO:0004152 - dihydroorotate dehydrogenase
                     activity [Evidence IEA];
                     GO_process: GO:0006222 - UMP biosynthetic process
                     [Evidence IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="quinone-dependent dihydroorotate dehydrogenase"
                     /protein_id="HOQ54197.1"
                     /translation="MNPRVSVVDAGYRAIVRPAMFGAYHGDPEAIHETMIANLARFSR
                     SPLALSATRLLAGSRGAPVTVAGIRFPGRVGVAAGLDKDGRAALAWQSMGFGFAELGT
                     VTAQAQPGNARPRVFRLKKSHALINRMGFNNAGAQRLHHQLSLLGIYRDNAGAGIPIG
                     ISIGKTKVVPVEAAVDDYLASLALVAPHADYVAINVSSPNTPGLRSLQDGGALLQLLR
                     ALVERAAGRLPLFVKVAPDLTESQLDEVLAAALDAGVAGLIATNTTLGRAGLDAADQP
                     LAAEAGGLSGAPLTVRAREVVGYLTRHTDLPVMAAGGIMTADDAAAMFDLGAPLVQLY
                     TGFIYGGPALPARINALTRQLRDAPAATRDTSSHRR"
     gene            complement(2835..>3137)
                     /locus_tag="PLF56_11375"
     CDS             complement(2835..>3137)
                     /locus_tag="PLF56_11375"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_013863521.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="hypothetical protein"
                     /protein_id="HOQ54198.1"
                     /translation="PGADGTKTVVQAIMDGEISLIFNTPLGVSEGGDPRRDGWQIRSA
                     AILCDVPCVTTVAGLSAAVQGIEALRRGGLTVRPLQAWSADMRRELAAAPASSAQG"
BASE COUNT          471 a         1115 c         1093 g          458 t
ORIGIN      
        1 ggcggtgacc agccggcgct cgcgctgctc ggcggtctcg gtgccgcgtc cgatcagtcg
       61 gttgaccaac tcgtcccagc tgggtggagc gatgaacacc agccgtgcct gtggcaggcg
      121 ctgccgcacc tgccgggcgc cctgcaggtc gatctctaga atcacccgct caccccgctc
      181 gaccgcctgc tgcaccggtg ccgacagggt gccgtagcga tcgcgtccgt gcaccaccgc
      241 ccactcgagc agaccatcgg tcgcgatcag ttcgtcgaag tcggcgtcac cgatgaacca
      301 gtagtgctcg ccgtttcgct cacccggccg gggtggacgg gtggtggccg agaccgacac
      361 ccacaactcg gggtgcagcg ccttgagtgc cttggtgacg gtgcctttgc ctacggccgt
      421 tgggccggag atgacggtga catcaccgct caccgaactc ggccttcagg ccggcgagct
      481 gatgccggcc aagtccgcgg atgcgccgat tggcggcgat gtcgtaccgc tccatgacca
      541 gcgtggcccg cttgtcgccc acccgtggga gcgccttcag caggtcgacc accttgacgt
      601 gggccagcac ctcgctgtcg gcggcctcgt cgagcgcctg cgccaaggac aactcacccg
      661 agcgcacccg cgccttgagt gccgcccggg atcgacgcgc ctcggtggcg gcggcccgag
      721 cagcctgcaa ctgctcggtt gtcagttggg gtatcgccac ggtgacctcc ggaccgctcg
      781 ccctgccttc gttttcgaca atttacaagc aaaccaacgt tgtgaacacc cttgcgggcg
      841 cgcatttcaa tgggtctggt cggcgacgcc gagcaaccgg tcaacgcggc cgttcagggc
      901 cgcagcatcg ggcccggcag cgatcacgtc gcggctgacc gccggcagca ccacgtcgat
      961 ggcgtcgccg aagagagcgg gcagatcgtc gatacggccg ccctgaaagc cgatccccgg
     1021 caccaggatc gagccgttga agcccgacac gtcacaaccc aggtcgtcgt gggtaccccc
     1081 caccaccagc ccgatggcac cctgcccgga cgcactgttg gcccgccggg ccgcgtcgat
     1141 gatctcttgg gcgaccgagc cctcgtggtc ttcgccgagc gccaactgca ccaggtgccc
     1201 ctcggggttc gaggtgcggg ccagcacgta cacgccccgt ccggaccgct cggcggcatc
     1261 gaaggccggc tgcaacgagc cgaaccccag gtagggcgac acggtgatcg catcggcggc
     1321 cagcggcgca ccgtcgacca ggtaggcgtc ggtgtaggca cccatggtcg agccgatgtc
     1381 gccgcgcttg acgtcgagaa tgctcaacgc gccggctgcg gcgatgtcgc cgagcacccg
     1441 ctccagcacg ccgataccgg ccgagccgtg ccgctcgaag aaggccgact gcggtttgaa
     1501 cacggccacc ttggcaccga gcacctcgac cactcgacgg gcgaacacct cgaggccgga
     1561 cgggtcgtcc gccagtcccc aggcctgcag caggcccggg tgggggtcga tgcccacgca
     1621 caggttgccg cgctcggtgg tgcgccgggc cagtcgctgc cgataggtgg tcatgctgtg
     1681 ctcctgatca atggtgctgc ggcgtgactg tcaggcctca gcgacggtgg ctggacgtgt
     1741 cgcgggtcgc cgcgggggca tcccggagtt ggcgggtgag cgcgttgatc cgcgccggca
     1801 gcgccgggcc gccatagatg aacccggtgt agagctgcac cagcggcgca cccaggtcga
     1861 acatcgcggc agcatcgtcg gcggtcatga tgccgccggc ggccatcacc ggcaggtcgg
     1921 tgtgccgggt gagatacccg accacctcgc gcgcgcgcac ggtcagcggt gcacccgaca
     1981 gcccgccggc ttcggcggcc aacggctggt cggccgcatc gagacccgca cgacccagcg
     2041 tggtgttggt cgcgatcagg ccggccacgc cggcgtccag ggccgccgcg agcacctcgt
     2101 cgagctgtga ttcggtcagg tcgggggcga ccttgacgaa cagcggcagg cgtccggcgg
     2161 cccgctcgac cagggcgcgc agcaactgca gcagcgctcc cccgtcctgc aggctgcgca
     2221 gcccgggtgt gttggggctc gagacgttga tcgccacgta gtcggcatgg ggtgcgacca
     2281 gggccaacga ggccaggtag tcgtcgacgg ccgcctcgac cggcaccacc ttggtcttgc
     2341 cgatcgagat gccgatcgga atgcccgcgc cggcgttgtc gcggtagatg ccgagcagcg
     2401 acaactgatg gtgcaggcgt tgcgcgcccg cgttgttgaa gcccatgcgg ttgatcagcg
     2461 cgtgcgactt cttcagccgg aacacccgtg gacgcgcgtt gcccggctgc gcctgggcgg
     2521 tgactgtgcc cagctcagcg aagccgaaac ccatcgactg ccaggccagc gcggctcgtc
     2581 cgtccttgtc gagaccggcg gccaccccga cgcggccggg gaaccggatg ccggccacgg
     2641 tgaccggcgc cccgcggctg ccggccagca gccgggtggc cgagagcgcc agcggcgagc
     2701 ggctgaaccg ggccaggttg gcgatcatgg tctcgtggat cgcctcgggg tcgccgtggt
     2761 aggcgccgaa cattgccggg cgcacgatgg cccgataccc ggcatcgacc acgctgaccc
     2821 gcggattcac tcgttcatcc ttgcgcggac gaggcgggcg cggcagccaa ttcgcgacgc
     2881 atgtcggcgc tccaggcctg cagtggacgc acggtcagcc cgccccggcg caacgcctca
     2941 atgccctgga cggccgcgga caggccggcg accgtggtca cgcacggcac gtcgcacaga
     3001 atggccgccg aacggatctg ccacccgtcc cggcgggggt cgccgccctc ggagacgccg
     3061 agcggggtgt tgaagatcag gctgatctcg ccgtccatga tcgcctggac gacggtcttg
     3121 gtgccgtccg cgccggg
//
LOCUS       DAOSUG010000380         3135 bp    DNA     linear   ENV 10-JUN-2024
DEFINITION  MAG TPA_asm: Micropruina sp. isolate AMR_MDS_2195
            NODE_12619_length_3135_cov_3.922403, whole genome shotgun sequence.
ACCESSION   DAOSUG010000380 DAOSUG010000000
VERSION     DAOSUG010000380.1
DBLINK      BioProject: PRJNA925477
            BioSample: SAMN32864262
            Sequence Read Archive: SRR4236673
KEYWORDS    WGS; ENV; Metagenome Assembled Genome; MAG; Third Party Data; TPA;
            TPA:assembly.
SOURCE      Micropruina sp. (wastewater metagenome)
  ORGANISM  Micropruina sp.
            Bacteria; Bacillati; Actinomycetota; Actinomycetes;
            Propionibacteriales; Nocardioidaceae; Micropruina.
REFERENCE   1  (bases 1 to 3135)
  AUTHORS   Abdulkadir,N., Saraiva,J.P., Zhang,J., Stolte,S., Gillor,O.,
            Harms,H. and Rocha,U.
  TITLE     Genome-centric analyses of 165 metagenomes show that mobile genetic
            elements are crucial for the transmission of antimicrobial
            resistance genes to pathogens in activated sludge and wastewater
  JOURNAL   Microbiol Spectr 12 (3), e0291823 (2024)
   PUBMED   38289113
REFERENCE   2  (bases 1 to 3135)
  AUTHORS   Abdulkadir,N.
  TITLE     Direct Submission
  JOURNAL   Submitted (24-MAR-2023) Environmental Microbiology, Helmholtz
            Center for Environmental Research -UFZ, Permoserstr 15, Leipzig
            04318, Germany
COMMENT     The annotation was added by the NCBI Prokaryotic Genome Annotation
            Pipeline (PGAP). Information about PGAP can be found here:
            https://www.ncbi.nlm.nih.gov/genome/annotation_prok/
            
            ##Genome-Assembly-Data-START##
            Assembly Method        :: SPAdes v. 3.11.1
            Genome Representation  :: Full
            Expected Final Version :: Yes
            Genome Coverage        :: Not Applicable
            Sequencing Technology  :: Illumina MiSeq
            ##Genome-Assembly-Data-END##
            
            ##Genome-Annotation-Data-START##
            Annotation Provider               :: NCBI
            Annotation Date                   :: 05/04/2023 04:57:10
            Annotation Pipeline               :: NCBI Prokaryotic Genome
                                                 Annotation Pipeline (PGAP)
            Annotation Method                 :: Best-placed reference protein
                                                 set; GeneMarkS-2+
            Annotation Software revision      :: 6.5
            Features Annotated                :: Gene; CDS; rRNA; tRNA; ncRNA
            Genes (total)                     :: 2,776
            CDSs (total)                      :: 2,750
            Genes (coding)                    :: 2,714
            CDSs (with protein)               :: 2,714
            Genes (RNA)                       :: 26
            tRNAs                             :: 24
            ncRNAs                            :: 2
            Pseudo Genes (total)              :: 36
            CDSs (without protein)            :: 36
            Pseudo Genes (ambiguous residues) :: 0 of 36
            Pseudo Genes (frameshifted)       :: 2 of 36
            Pseudo Genes (incomplete)         :: 32 of 36
            Pseudo Genes (internal stop)      :: 2 of 36
            ##Genome-Annotation-Data-END##
FEATURES             Location/Qualifiers
     source          1..3135
                     /organism="Micropruina sp."
                     /mol_type="genomic DNA"
                     /submitter_seqid="NODE_12619_length_3135_cov_3.922403"
                     /isolate="AMR_MDS_2195"
                     /isolation_source="Activated sludge of municipal
                     wastewater treatment plants"
                     /db_xref="taxon:2737536"
                     /environmental_sample
                     /geo_loc_name="Canada"
                     /lat_lon="49.25 N 123.1 W"
                     /collection_date="2015-06-16"
                     /metagenome_source="wastewater metagenome"
                     /note="metagenomic"
     gene            complement(<1..691)
                     /locus_tag="PLF56_11380"
     CDS             complement(<1..691)
                     /locus_tag="PLF56_11380"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_020389511.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_process: GO:0046855 - inositol phosphate
                     dephosphorylation [Evidence IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="inositol monophosphatase family protein"
                     /protein_id="HOQ54199.1"
                     /translation="MPEQSYLDDLRLAHMMADNADSLTMSRFKALDLKVDAKPDLTPV
                     SDADVAVEDAIRRTLKTARPRDAVHGEEREDTGWGPRRWVIDPIDGTANFVRGVPVWA
                     TLIGLMVEGRVVVGVVSAPALGRRWWASAGGGAFTGKTLLQGNPCRVSEVGSIDDSSL
                     SYSSVHGWVDSGRGQQFVDLMRGCWRTRAYGDFWSYMLVAEGAVDIATEPELALHDMA
                     ALDVIVREAGGR"
     gene            complement(759..1766)
                     /gene="rsgA"
                     /locus_tag="PLF56_11385"
     CDS             complement(759..1766)
                     /gene="rsgA"
                     /locus_tag="PLF56_11385"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_013863977.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_function: GO:0005525 - GTP binding [Evidence IEA];
                     GO_function: GO:0043022 - ribosome binding [Evidence IEA];
                     GO_process: GO:0006412 - translation [Evidence IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="ribosome small subunit-dependent GTPase A"
                     /protein_id="HOQ54200.1"
                     /translation="MSQRLRGGVSDDDHAGFGRPGRGSRPRTKDRPDYSKAAEAVVVT
                     IDRGRYRCLHDEVALTATKARVLGRKGVIVGDRVRVVGDLSGAEGTLARIVEVLPRRT
                     VLRRTADDTDPFERPIVANADQLVIVTALADPPPRMGMIDRILVAAYDAGLDPVLCLT
                     KQDLASADELTAAYAPLEIPIVAVQPGADLSAVRALLRDRVSVFVGHSGVGKSTLVNA
                     LIPGVERAIGEVNEVTGRGRHTSTAAIALELPEHAGWVIDTPGVRSFGLSHVTPASIV
                     AAFPDLAELTAGCPRGCTHASGVPECALDEAVAQGRLAPERLDSFRRMEGAWLERPAG
                     S"
     gene            complement(1774..3036)
                     /gene="aroA"
                     /locus_tag="PLF56_11390"
     CDS             complement(1774..3036)
                     /gene="aroA"
                     /locus_tag="PLF56_11390"
                     /EC_number="2.5.1.19"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_020389508.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_function: GO:0003866 - 3-phosphoshikimate
                     1-carboxyvinyltransferase activity [Evidence IEA];
                     GO_process: GO:0009423 - chorismate biosynthetic process
                     [Evidence IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="3-phosphoshikimate 1-carboxyvinyltransferase"
                     /protein_id="HOQ54201.1"
                     /translation="MTSAAALASAGPLSATIVVPGSKSATARGYVLAALASGPSVLTG
                     VLDSRDTRLMRAALAGLGVGFTDLSDGVVQVTPPSRFTPAAAQVGLAGTIMRFVPPIA
                     ALAEGASRFEGDDEAAARPVFPLLDGLAQAGVRVEHPRSLPFVVHGTGVVPGGAVTID
                     ASGSSQFVSGLLLAGARFEQGLDLRHEGGTLPSRPHIDLTLAMLRERGVRVTSPSENR
                     WVVSPGPIAAAHDTIEPDLINAATFLAAPLIAGGSVTTAWPTHTVQAADAILQVIGAL
                     GGDVRLHDGRVTVTGTGRVAAADLDLARTSELTCIAAALLSLADAPGSIRGVGHVRGH
                     ETDRLAALQAELNALGGRVAQTADGLAIDPAPLAPGLWHTYADHRMAHAGALLGLAVP
                     GVELDDLTCTTKTLPDFSGLWQRMLSQR"
BASE COUNT          448 a         1159 c         1107 g          421 t
ORIGIN      
        1 atcgaccccc cgcctcgcgc acgatcacgt cgagcgctgc catgtcgtgc aatgccaact
       61 ccggctcggt ggcgatgtcg acggctccct cggcgaccag catgtagctc cagaagtccc
      121 cgtaggcccg ggtgcgccag cagccgcgca tcaggtcgac gaactgttgg ccgcgcccgc
      181 tgtccaccca accgtgcacc gacgagtacg acagggagga atcatcgatc gaaccgacct
      241 cggacacccg gcaggggttg ccctgcagca gcgtcttgcc ggtgaacgca cccccgccgg
      301 cgctggccca ccagcgtcgt cccagggcgg gcgccgacac cacaccgacc accacccggc
      361 cctcgaccat caacccgatc agcgtggccc acaccggtac tccgcggacg aagttggccg
      421 tgccgtcgat cgggtcgatc acccagcgac gcggacccca accggtgtcc tcgcgctcct
      481 caccgtgcac cgcatcccgc ggacgggccg tcttcagcgt gcgccggatc gcgtcctcga
      541 ccgccacatc ggcgtcgctc accggggtca ggtcgggttt ggcgtccacc ttgaggtcga
      601 gggccttgaa cctgctcatg gtcagcgaat cggcgttgtc ggccatcatg tgcgccagcc
      661 gtaggtcgtc gaggtagctc tgctcgggca tgcctctcaa cctatcggtt gcccgcgcca
      721 ccagtgggga cgtacccgtg ccgcgccccg cccgaacgtc agctgccggc cggacgctcc
      781 agccacgcgc cctccatgcg gcggaacgag tccagccgct cgggcgccag ccgtccctgc
      841 gccacggcct cgtccagggc gcactccggc acccccgagg cgtgggtgca gccgcgcggg
      901 cacccggcgg tcagctcggc gagatcggga aaggccgcga cgatcgaggc cggcgtcaca
      961 tggctgagcc cgaacgaccg caccccgggg gtgtcgatca cccacccggc atgctcgggc
     1021 agttcgaggg cgatcgccgc ggtggaggtg tggcggcccc gtccggtgac ctcgttgacc
     1081 tcgccgatgg cccgctccac accggggatc agcgcgttca ccagggtgga tttgcccacc
     1141 ccggagtggc cgacgaacac gctcacgcga tcgcgcagca acgcacgcac cgcactgagg
     1201 tcggccccgg gctgcacggc gacgatcgga atctccagcg gcgcgtaggc ggcggtcagc
     1261 tcgtcggccg aggccaggtc ctgcttggtc aggcacagca ccgggtcgag gccggcgtcg
     1321 taggcggcga ccagaatgcg gtcgatcatg cccatccgcg gcggcggatc ggcgagcgcg
     1381 gtgacgatca ccagctgatc ggcattggcc acgatcggcc gttcgaacgg gtcggtgtca
     1441 tcggcggtgc ggcgcagcac ggtacgccga ggcagcacct cgacgatgcg ggccagggtg
     1501 ccctcggcgc cgctcaggtc gcccaccaca cgcacccggt cgccgacgat cacacccttg
     1561 cggcccagca cccgggcctt cgtcgcggtc agcgccacct cgtcgtgcag gcagcggtac
     1621 cgcccgcgat cgatcgtcac caccacggcc tcggcggcct tggaatagtc gggacggtcc
     1681 ttggtgcggg ggcgcgagcc gcgcccgggg cgtccgaaac cggcgtggtc gtcgtcgctg
     1741 acgccgcccc gcaaccgctg gctcaccgtc gcctcaccgc tggctcagca tgcgctgcca
     1801 caggccggaa aagtcgggca gggtcttggt ggtgcaggtg agatcgtcga gctccacgcc
     1861 tggcacggcc agccccagca gggccccggc atgcgccatg cggtggtcgg cataggtgtg
     1921 ccagaggccg ggagccagcg gcgcggggtc gatggccagg ccgtccgcgg tctgggcgac
     1981 ccggccgccc agggcgttca gttcggcctg cagcgcggcc agccggtccg tctcgtgacc
     2041 ccggacgtgc cccaccccgc gaatgctgcc cggagcgtcc gccaggctga gcagggcggc
     2101 ggcgatgcag gtgagttcgc tggtgcgggc caggtcgaga tcggcggcgg cgacgcgtcc
     2161 ggtgcccgtg acggtgaccc gcccgtcgtg cagccgcacg tcgccgccca gtgcaccgat
     2221 cacctgcagg atcgcgtcgg cggcctgcac ggtgtgcgtg ggccaggcgg tggtcacgct
     2281 gccgccggcg atcaacggcg ccgccaggaa tgtggcggcg ttgatcaggt cgggctcgat
     2341 ggtgtcgtgg gcggccgcga tcgggcccgg gctcaccacc caccggttct cggacgggct
     2401 cgtcacgcgc accccgcgct cacgcagcat ggcgagcgtc aagtcgatgt gcggcctgga
     2461 cggcagcgtg ccgccctcgt gccgcaggtc gagtccctgc tcgaaccggg cgccggcgag
     2521 cagcaggccc gagacgaact ggctcgagcc cgacgcatca atggtgacgg cgccgccggg
     2581 cacaactccg gtgccgtgca ccacgaacgg cagcgaccgg gggtgttcga cgcgcacccc
     2641 ggcctgggcc agcccgtcca gcagcggaaa gaccggacgt gcggcggcct cgtcatcgcc
     2701 ctcgaaccgg ctggcacctt cggcgagggc ggcgatcggg ggcacgaacc gcatgatcgt
     2761 gccggccagc cccacctgcg cagcggcggg ggtgaatcgg gacgggggag tgacctgcac
     2821 cacgccgtcc gagaggtcgg tgaagccgac gcccagcccg gccagcgcgg ctcgcatcag
     2881 ccgggtgtcg cgtgaatcga gcacgccggt gagcacgctg gggcccgacg ccagggcggc
     2941 cagcacgtag cctcgggcgg tggccgactt cgagccgggc acgacgatcg tcgccgacag
     3001 cggcccggcg ctcgccaggg ccgccgccga ggtcacttca gcgcaccttc aagcgtcttg
     3061 gtggcctggt tagcaacctt cttggccttg cgggcggcct tgcggccctc acgctccagc
     3121 gatttcttgg tgcgc
//
LOCUS       DAOSUG010000381         3120 bp    DNA     linear   ENV 10-JUN-2024
DEFINITION  MAG TPA_asm: Micropruina sp. isolate AMR_MDS_2195
            NODE_12681_length_3120_cov_3.634910, whole genome shotgun sequence.
ACCESSION   DAOSUG010000381 DAOSUG010000000
VERSION     DAOSUG010000381.1
DBLINK      BioProject: PRJNA925477
            BioSample: SAMN32864262
            Sequence Read Archive: SRR4236673
KEYWORDS    WGS; ENV; Metagenome Assembled Genome; MAG; Third Party Data; TPA;
            TPA:assembly.
SOURCE      Micropruina sp. (wastewater metagenome)
  ORGANISM  Micropruina sp.
            Bacteria; Bacillati; Actinomycetota; Actinomycetes;
            Propionibacteriales; Nocardioidaceae; Micropruina.
REFERENCE   1  (bases 1 to 3120)
  AUTHORS   Abdulkadir,N., Saraiva,J.P., Zhang,J., Stolte,S., Gillor,O.,
            Harms,H. and Rocha,U.
  TITLE     Genome-centric analyses of 165 metagenomes show that mobile genetic
            elements are crucial for the transmission of antimicrobial
            resistance genes to pathogens in activated sludge and wastewater
  JOURNAL   Microbiol Spectr 12 (3), e0291823 (2024)
   PUBMED   38289113
REFERENCE   2  (bases 1 to 3120)
  AUTHORS   Abdulkadir,N.
  TITLE     Direct Submission
  JOURNAL   Submitted (24-MAR-2023) Environmental Microbiology, Helmholtz
            Center for Environmental Research -UFZ, Permoserstr 15, Leipzig
            04318, Germany
COMMENT     The annotation was added by the NCBI Prokaryotic Genome Annotation
            Pipeline (PGAP). Information about PGAP can be found here:
            https://www.ncbi.nlm.nih.gov/genome/annotation_prok/
            
            ##Genome-Assembly-Data-START##
            Assembly Method        :: SPAdes v. 3.11.1
            Genome Representation  :: Full
            Expected Final Version :: Yes
            Genome Coverage        :: Not Applicable
            Sequencing Technology  :: Illumina MiSeq
            ##Genome-Assembly-Data-END##
            
            ##Genome-Annotation-Data-START##
            Annotation Provider               :: NCBI
            Annotation Date                   :: 05/04/2023 04:57:10
            Annotation Pipeline               :: NCBI Prokaryotic Genome
                                                 Annotation Pipeline (PGAP)
            Annotation Method                 :: Best-placed reference protein
                                                 set; GeneMarkS-2+
            Annotation Software revision      :: 6.5
            Features Annotated                :: Gene; CDS; rRNA; tRNA; ncRNA
            Genes (total)                     :: 2,776
            CDSs (total)                      :: 2,750
            Genes (coding)                    :: 2,714
            CDSs (with protein)               :: 2,714
            Genes (RNA)                       :: 26
            tRNAs                             :: 24
            ncRNAs                            :: 2
            Pseudo Genes (total)              :: 36
            CDSs (without protein)            :: 36
            Pseudo Genes (ambiguous residues) :: 0 of 36
            Pseudo Genes (frameshifted)       :: 2 of 36
            Pseudo Genes (incomplete)         :: 32 of 36
            Pseudo Genes (internal stop)      :: 2 of 36
            ##Genome-Annotation-Data-END##
FEATURES             Location/Qualifiers
     source          1..3120
                     /organism="Micropruina sp."
                     /mol_type="genomic DNA"
                     /submitter_seqid="NODE_12681_length_3120_cov_3.634910"
                     /isolate="AMR_MDS_2195"
                     /isolation_source="Activated sludge of municipal
                     wastewater treatment plants"
                     /db_xref="taxon:2737536"
                     /environmental_sample
                     /geo_loc_name="Canada"
                     /lat_lon="49.25 N 123.1 W"
                     /collection_date="2015-06-16"
                     /metagenome_source="wastewater metagenome"
                     /note="metagenomic"
     gene            complement(<1..390)
                     /locus_tag="PLF56_11395"
     CDS             complement(<1..390)
                     /locus_tag="PLF56_11395"
                     /EC_number="1.1.1.1"
                     /inference="COORDINATES: protein motif:HMM:NF012678.2"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_function: GO:0016491 - oxidoreductase activity
                     [Evidence IEA];
                     GO_function: GO:0046872 - metal ion binding [Evidence
                     IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="iron-containing alcohol dehydrogenase"
                     /protein_id="HOQ54202.1"
                     /translation="MTSFTFATAQRIVFGPGRAADLATVIGEFGRRPFVCTGADPGRF
                     AHLLAGLDDVGTWAVAGEPTLDDVRAGVAAARAHGADVLIGLGGGAVLDSAKLIAALV
                     PGDGDVLDHLEVIGAGRPLAATPLPFIA"
     gene            574..2076
                     /locus_tag="PLF56_11400"
     CDS             574..2076
                     /locus_tag="PLF56_11400"
                     /EC_number="3.4.11.1"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_014847087.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_function: GO:0030145 - manganese ion binding [Evidence
                     IEA];
                     GO_function: GO:0070006 - metalloaminopeptidase activity
                     [Evidence IEA];
                     GO_process: GO:0006508 - proteolysis [Evidence IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="leucyl aminopeptidase"
                     /protein_id="HOQ54203.1"
                     /translation="MTSFTLPSLTLATAAPADADVVVIGLADGASTPVLVGASDQLQK
                     RVAKQFGRALLEVALSLGGTTKPGVTVTLPGPDGTRVVVVGLGDLDVTPEQVRRAAGN
                     GVRQAAALAGDGSVTVAVCLDAVEPDVIRGAAEGAVLGAYSFVKVSGSEPAPSVAAIT
                     VVSSSGKPEARRAVETARIVATAVCLARDWVNAPANVLYPETFAESARAAAKGTRIEV
                     EVLDDKALERGAYGGLMAVGGGSSRPPRLVRFSYAPRGAKTHLALVGKGITFDTGGLN
                     LKPADGMYTMKNDMAGAAAVLAATRAIADLGLKVRVTSYAAMAENMPSGTAYRPSDVL
                     TMYGGTTVENVNSDAEGRLVMADALVRAGEDSPDLILDVATLTGACVVALGERVAGLM
                     ASADEAADRVLDAAESAGEAFWQLPIPDGTRKKLDSKVADLRSGGNRAGGALTAAAFL
                     QTFVPEGTLWAHLDIAGPAFNDEGPYDYVPSGGTGVAVRTLVALAQSLQS"
     gene            2082..>3120
                     /locus_tag="PLF56_11405"
     CDS             2082..>3120
                     /locus_tag="PLF56_11405"
                     /inference="COORDINATES: protein motif:HMM:NF013086.2"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_function: GO:0016491 - oxidoreductase activity
                     [Evidence IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="FAD-dependent oxidoreductase"
                     /protein_id="HOQ54204.1"
                     /translation="MSQMRTDVIIVGAGAAGLLAAVAARRLGHEVLVVEASSVVGGTT
                     ATTDGALWLPANDLMGRGGIPSDSPDVALRYLNGLSAPDSPAIAARRQAFVRTAAAVG
                     RWLSTSKIALEVVKSLPDCRPDADGAAAQGRTVRVRPVAAKLPDDWRDRVRGAGQGDA
                     KPGLLARLTRPFRDPAQPAGGACLIGELLRRALGSGVEVWLDAPLHALLVDDGTVTGA
                     VVQRGDERVEVHAERGVMLACGGFEGDQSLRQEHLPLPTSSAWSVGLGAEGTALAAGA
                     QVAAAVAGLDQAWWTPVLVADGVAHKVDAARVAPHSLIVDQAGDRYLNEAQPSVKVTP
                     PQSLAQADCWQA"
BASE COUNT          426 a         1115 c         1149 g          430 t
ORIGIN      
        1 ggcgatgaac ggcagcggcg ttgcggccag cggacgtccg gcaccgatca cctcgaggtg
       61 atcgagcacg tcaccgtcgc ccggcaccag ggcggcgatc agcttggcgg agtccagcac
      121 cgcgccacca ccgaggccga tcagcacgtc ggcgccgtgg gcccgggccg ccgcgacccc
      181 ggcccggaca tcgtccaggg tcggttcgcc cgcgaccgcc caggtgccga cgtcgtccag
      241 gccggccagc aggtgcgcga accggcccgg atcggcaccg gtgcacacga acggacggcg
      301 cccgaactcg ccgatcacgg tggccaggtc ggctgcccgt ccgggcccga agacgatgcg
      361 ttgcgcggtg gcgaaggtga aactggtcac ggtgccaaca atgacacgtc ggacggcgtg
      421 cgacccggcc atcgcagaca gatcacctgg gcgggcttga cggtgtccga cccggccacg
      481 gcggcgcgca tcgccccggc ggcgctgcgt ggatcggggc tacagtgacc agtgacttca
      541 cccgtcaggc ttgctcaccg ataggacccg tccgtgacct cgttcaccct gccttcactc
      601 acgctggcca ccgccgcccc cgccgatgcc gatgtggtgg tgatcggtct cgccgacggc
      661 gcctcgacgc cggtgctcgt cggcgcctct gaccagttgc agaagcgggt ggccaagcag
      721 ttcggacgcg cgctgctgga ggtggcgctg tccctgggcg gcaccaccaa gccgggcgtc
      781 acggtgacgt tgcccggccc ggacggcacc cgcgtcgtgg tggtcggact cggcgatctg
      841 gacgtcaccc ccgagcaggt ccggcgagcc gccggcaacg gcgtgcggca ggccgcggcc
      901 ctggccggcg acggatcggt cacggtggcc gtctgcctgg acgcggtcga gcccgacgtg
      961 atccggggcg ccgccgaagg tgccgtgctc ggcgcctatt cgttcgtcaa ggtcagcggc
     1021 agcgagccgg cgccgtccgt cgcggcgatc accgtggtga gcagctcggg caagcccgag
     1081 gctcgccgtg ccgtcgaaac cgcgcgcatc gtggccaccg ccgtgtgcct ggcccgcgac
     1141 tgggtgaacg cccccgccaa cgtgctgtac cccgagacct tcgccgaatc cgcccgagcc
     1201 gcggccaagg gcacccgcat cgaggtcgag gtcctcgacg acaaggccct ggagcggggt
     1261 gcctacggcg ggctgatggc cgtcggcggc ggatcgtcac ggcccccgcg cctggtgcgg
     1321 ttctcgtacg ccccccgcgg agcgaagacg catctggcgc tggtcggcaa gggcatcacc
     1381 ttcgacaccg gcggtctcaa cctgaagccg gccgacggta tgtacacgat gaagaacgac
     1441 atggcgggcg ccgccgcggt gctggccgcc acccgcgcca tcgccgacct cggcctgaaa
     1501 gtgcgggtga ccagctacgc ggcgatggcc gagaacatgc cgtccggcac cgcgtaccgt
     1561 ccctcggacg tgctgaccat gtacggcggc acgacggtcg agaacgtcaa ctccgatgcc
     1621 gagggccggt tggtgatggc ggacgcgttg gtgcgagccg gtgaggattc ccccgacctg
     1681 attctcgacg tcgccaccct gaccggggcc tgcgtggtcg cgctcggcga gcgggtggcc
     1741 gggctgatgg ccagtgcgga cgaggccgcc gatcgcgtac tcgatgccgc cgagtccgcc
     1801 ggtgaggcgt tctggcagtt gccgattccg gacggaaccc gcaagaagct cgactcgaag
     1861 gtggccgacc tgcgctccgg cggcaaccgc gccggcggcg ccctcaccgc cgccgccttt
     1921 ctgcagacct tcgtgcccga aggcacgttg tgggcgcacc tcgacatcgc cggcccggcg
     1981 ttcaacgacg aaggccccta cgactacgtg ccctcgggcg gtaccggggt ggccgtgcgc
     2041 accttggtgg cactggcgca gtcgttgcag agctgagtcg gatgagccag atgcgcaccg
     2101 atgtgatcat cgtcggcgcc ggagcggcgg gcctgttggc cgccgtggcc gcccgcaggc
     2161 tggggcacga ggtgctcgtg gtcgaggcct cctccgtcgt cggcggcacc actgccacca
     2221 ccgacggtgc cctgtggcta ccggcgaacg acctgatggg ccgcggcggt attccgtccg
     2281 acagtccgga cgtggcgctg cgctacctca acggcctgtc cgctcccgac tcgccggcga
     2341 tcgccgcccg gcgccaggct ttcgtgcgca cggccgccgc ggtcggacgc tggctgagca
     2401 cgtccaagat cgcgctcgaa gtggtcaagt cgctgccgga ctgccgcccg gacgccgacg
     2461 gtgccgccgc gcagggccgc acggtgcggg tgcgtccggt ggccgcgaag ctgcccgacg
     2521 actggcgtga ccgggtgcgc ggggccggcc agggcgatgc caagcccggg ctgctggcgc
     2581 gcctgacgcg accgttccgt gatcctgccc agccggccgg gggcgcctgc ctgatcggcg
     2641 agctgttgcg ccgcgccctc ggcagcggcg tcgaggtctg gctggacgca ccgctgcacg
     2701 cactgctcgt cgacgacgga acggtcaccg gcgcggtcgt ccagcgtggt gacgagcggg
     2761 tcgaagtgca tgccgagcgg ggtgtgatgc tggcctgcgg ggggttcgag ggcgatcaat
     2821 cgttgcgcca ggagcatctg ccgctgccca cgtcgtccgc ctggtcagtg gggctgggtg
     2881 ccgaggggac ggcgctggcc gctggggcgc aggtcgcagc cgccgtcgcg gggctggatc
     2941 aggcctggtg gacgccggtg ctggtggccg acggggtcgc gcacaaggtg gacgccgcac
     3001 gcgtcgctcc gcacagcctg atcgtcgacc aggccggtga ccggtacctg aacgaggccc
     3061 aaccgtcggt gaaagtcaca ccaccccaga gcctggccca ggccgactgc tggcaagccc
//
LOCUS       DAOSUG010000382         3105 bp    DNA     linear   ENV 10-JUN-2024
DEFINITION  MAG TPA_asm: Micropruina sp. isolate AMR_MDS_2195
            NODE_12757_length_3105_cov_3.297705, whole genome shotgun sequence.
ACCESSION   DAOSUG010000382 DAOSUG010000000
VERSION     DAOSUG010000382.1
DBLINK      BioProject: PRJNA925477
            BioSample: SAMN32864262
            Sequence Read Archive: SRR4236673
KEYWORDS    WGS; ENV; Metagenome Assembled Genome; MAG; Third Party Data; TPA;
            TPA:assembly.
SOURCE      Micropruina sp. (wastewater metagenome)
  ORGANISM  Micropruina sp.
            Bacteria; Bacillati; Actinomycetota; Actinomycetes;
            Propionibacteriales; Nocardioidaceae; Micropruina.
REFERENCE   1  (bases 1 to 3105)
  AUTHORS   Abdulkadir,N., Saraiva,J.P., Zhang,J., Stolte,S., Gillor,O.,
            Harms,H. and Rocha,U.
  TITLE     Genome-centric analyses of 165 metagenomes show that mobile genetic
            elements are crucial for the transmission of antimicrobial
            resistance genes to pathogens in activated sludge and wastewater
  JOURNAL   Microbiol Spectr 12 (3), e0291823 (2024)
   PUBMED   38289113
REFERENCE   2  (bases 1 to 3105)
  AUTHORS   Abdulkadir,N.
  TITLE     Direct Submission
  JOURNAL   Submitted (24-MAR-2023) Environmental Microbiology, Helmholtz
            Center for Environmental Research -UFZ, Permoserstr 15, Leipzig
            04318, Germany
COMMENT     The annotation was added by the NCBI Prokaryotic Genome Annotation
            Pipeline (PGAP). Information about PGAP can be found here:
            https://www.ncbi.nlm.nih.gov/genome/annotation_prok/
            
            ##Genome-Assembly-Data-START##
            Assembly Method        :: SPAdes v. 3.11.1
            Genome Representation  :: Full
            Expected Final Version :: Yes
            Genome Coverage        :: Not Applicable
            Sequencing Technology  :: Illumina MiSeq
            ##Genome-Assembly-Data-END##
            
            ##Genome-Annotation-Data-START##
            Annotation Provider               :: NCBI
            Annotation Date                   :: 05/04/2023 04:57:10
            Annotation Pipeline               :: NCBI Prokaryotic Genome
                                                 Annotation Pipeline (PGAP)
            Annotation Method                 :: Best-placed reference protein
                                                 set; GeneMarkS-2+
            Annotation Software revision      :: 6.5
            Features Annotated                :: Gene; CDS; rRNA; tRNA; ncRNA
            Genes (total)                     :: 2,776
            CDSs (total)                      :: 2,750
            Genes (coding)                    :: 2,714
            CDSs (with protein)               :: 2,714
            Genes (RNA)                       :: 26
            tRNAs                             :: 24
            ncRNAs                            :: 2
            Pseudo Genes (total)              :: 36
            CDSs (without protein)            :: 36
            Pseudo Genes (ambiguous residues) :: 0 of 36
            Pseudo Genes (frameshifted)       :: 2 of 36
            Pseudo Genes (incomplete)         :: 32 of 36
            Pseudo Genes (internal stop)      :: 2 of 36
            ##Genome-Annotation-Data-END##
FEATURES             Location/Qualifiers
     source          1..3105
                     /organism="Micropruina sp."
                     /mol_type="genomic DNA"
                     /submitter_seqid="NODE_12757_length_3105_cov_3.297705"
                     /isolate="AMR_MDS_2195"
                     /isolation_source="Activated sludge of municipal
                     wastewater treatment plants"
                     /db_xref="taxon:2737536"
                     /environmental_sample
                     /geo_loc_name="Canada"
                     /lat_lon="49.25 N 123.1 W"
                     /collection_date="2015-06-16"
                     /metagenome_source="wastewater metagenome"
                     /note="metagenomic"
     gene            <1..748
                     /locus_tag="PLF56_11410"
     CDS             <1..748
                     /locus_tag="PLF56_11410"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_011751549.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=2
                     /transl_table=11
                     /product="rhodanese-like domain-containing protein"
                     /protein_id="HOQ54205.1"
                     /translation="GSARPLPAPVGTAIVRVGALTIAMTGANREELERAGIAFHTLHL
                     HPNQHAGYFPGAKQLHLVVHVASDDARLLGAQAIGPDGVAKRIDVLATAIRAGLRVTD
                     LIDLDLAYAPPYGQAKDAVNLVGMVGANVLDGTLRLWYAQQLDQVQGSALLLDVRSAT
                     EVATGTLPGALMVPHTELRGRLDEVREAAAGRPVRVLCASGVRSAIAHRILTQSGFDS
                     ASLSGGMLTLRSWLGAEAAGRLVTPALVSV"
     gene            792..1067
                     /locus_tag="PLF56_11415"
     CDS             792..1067
                     /locus_tag="PLF56_11415"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_011753637.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="metal-sensitive transcriptional regulator"
                     /protein_id="HOQ54206.1"
                     /translation="MPPTDPAVQRRIRNRLRRAQGQLDALVRAVEGGADCRQVVTQLA
                     AVSKALNRAGFAIVVSAMKECLADPDGNHPEEGITTAELEDLFLALA"
     gene            complement(1341..1526)
                     /locus_tag="PLF56_11420"
     CDS             complement(1341..1526)
                     /locus_tag="PLF56_11420"
                     /inference="COORDINATES: ab initio
                     prediction:GeneMarkS-2+"
                     /note="Derived by automated computational analysis using
                     gene prediction method: GeneMarkS-2+."
                     /codon_start=1
                     /transl_table=11
                     /product="hypothetical protein"
                     /protein_id="HOQ54207.1"
                     /translation="MIGLIPLDTLPGWEPAADPTALQTLTVLLGIPTAIFAVILLLTM
                     APSWFHRGGDVAVRTDD"
     gene            1758..2303
                     /gene="pyrR"
                     /locus_tag="PLF56_11425"
     CDS             1758..2303
                     /gene="pyrR"
                     /locus_tag="PLF56_11425"
                     /EC_number="2.4.2.9"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_013864550.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_process: GO:0009116 - nucleoside metabolic process
                     [Evidence IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="bifunctional pyr operon transcriptional
                     regulator/uracil phosphoribosyltransferase PyrR"
                     /protein_id="HOQ54208.1"
                     /translation="MTNPSARIAWEGHQITRAVARMAHQIVEANGGSEHVVLLGIPTR
                     GVLLARRLADAIRGIEGEAVAVGELDITMYRDDLRRQPTRAVGRTSIPRSIDDTTVVL
                     VDDVLFTGRTIAAALDALKEVGRPRMVQLAVLIDRGHRQLPIQADHVGRLLQVPAEAR
                     VNVRMAEIDEVDAVTISLPEA"
     gene            2303..>3105
                     /locus_tag="PLF56_11430"
     CDS             2303..>3105
                     /locus_tag="PLF56_11430"
                     /EC_number="2.1.3.2"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_014846875.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_function: GO:0004070 - aspartate carbamoyltransferase
                     activity [Evidence IEA];
                     GO_function: GO:0016597 - amino acid binding [Evidence
                     IEA];
                     GO_process: GO:0006207 - 'de novo' pyrimidine nucleobase
                     biosynthetic process [Evidence IEA];
                     GO_process: GO:0006520 - amino acid metabolic process
                     [Evidence IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="aspartate carbamoyltransferase catalytic
                     subunit"
                     /protein_id="HOQ54209.1"
                     /translation="MRHLLSAGDLSLAEVTSILDVAEEMHTIQQRQIKKLPTLRGRTV
                     INMFFEDSTRTRTSFEMAGKWMSADTINISAKGSSTSKGESLRDTMLTLDAMGTDCFV
                     IRHGSSGAVAQAASWVRAHVVNAGDGTHEHPTQALLDAFAMRRHFGTLGASDSGGQEA
                     FAGKRIAIVGDLLHSRVVRSNVLLQRTLGSSVTLVAPPTLLPTGVEQWGVGVTSDLDE
                     VIESVDVVMMLRVQRERMHGGFFPTEREYTELYGLTPERLARLRPGAAI"
BASE COUNT          481 a         1101 c         1065 g          458 t
ORIGIN      
        1 gggttcggcc cgtccgttgc cggcgccggt cggtacggcc atcgtccggg tcggcgccct
       61 gaccatcgcc atgaccggcg ccaaccgaga ggagttggag cgggcgggca tcgccttcca
      121 caccctgcac ctgcacccca accagcacgc cggctacttt ccgggggcca agcaactcca
      181 cctggtggtg catgtcgcca gcgatgacgc ccgcctgctg ggggctcagg cgatcggtcc
      241 ggacggcgtc gccaagcgca tcgacgtgct ggccacggcg atccgggccg ggctgcgggt
      301 gaccgacctg atcgatctcg acctcgccta cgcgccgccc tacggccagg ccaaggacgc
      361 ggtcaacctg gtcggcatgg tcggcgccaa cgtgctcgac ggcacgctac ggctctggta
      421 cgcccagcaa ctcgaccagg tgcagggctc ggcgctgctg ctcgacgtgc gctctgcgac
      481 cgaggtcgcc accggtaccc tgcctggagc cctgatggtt ccgcacaccg aactgcgcgg
      541 acggctggac gaggtgcgcg aggcggcggc cggccggccg gtgcgggtgc tgtgtgcgtc
      601 cggcgtccgc tcggcgatcg cccaccgaat cctcacccag tcgggtttcg actccgcgtc
      661 gttgtcgggc ggcatgctga ccctgcgatc ctggttgggc gccgaggcgg ccggccggct
      721 ggtcaccccc gccctcgtgt ccgtctgatc cgtccgagct gaccggctcg cgtcaccagc
      781 aggaggaacc catgccaccg accgaccccg ccgtccagcg ccgcatccgc aaccggctgc
      841 gccgcgccca gggccaactc gatgcgctgg tgcgcgccgt ggagggcggc gccgactgcc
      901 gacaggtggt cacccagctg gccgccgttt ccaaggcgct caaccgggcc ggcttcgcca
      961 tcgtggtgtc cgccatgaag gaatgcctgg ccgacccgga cggcaaccat cccgaggaag
     1021 gcatcaccac cgccgaactc gaggacctct tcctcgccct cgcctgagcg cgcccgactc
     1081 gacccctacg tcccatcgac gaccggcagg agcatccgaa cgatgtgtta cgcagtgaaa
     1141 tgcacgcagt gcggcaagac cacctgggcc ggctgcggcc agcatgtggc atcggtgaag
     1201 gccggcgttc cggccgccca atggtgcggc ggtcacgcgc cccaggccac acggtcgggc
     1261 ggagggctgc tcgggcgcct gttcggccgc tgagccgaga ttcagccgct gagcccagat
     1321 tcgggctgag tgggcgagat tcagtcgtcg gtgcggacgg cgacgtcgcc accccggtgg
     1381 aaccaactgg gcgccatggt cagcagcaga atcacggcga agatggccgt cggaatgccg
     1441 agcagcacgg tgagggtctg gagggcggtc gggtcggccg ccggttccca gccgggcagg
     1501 gtgtcgagcg ggatcagtcc gatcatgggt gacagggtat tgcaccgccg gcacggggtc
     1561 gagcgtgtgg gggtcgtcca gccgaacgcc caccgaacta tctcgcgccc gtgaagcttc
     1621 gcaggcgcat gatggttcgg tgggtgttcg gcggacgggt gtgacggacg ctcgtcccga
     1681 acgctagatt gaagccccga ggcgtcccca acctactcgg gtgcggcgtc gccgacgacc
     1741 ccgctaggag cattcgcgtg accaacccat ccgcccgcat cgcgtgggaa ggccatcaga
     1801 tcacgcgtgc cgtggcccgc atggcccatc agatcgtcga ggccaacggc ggcagtgagc
     1861 atgtcgtgct gctcggcatt ccgacccggg gggtactgct ggcccgccgg ctggccgatg
     1921 cgatccgcgg catcgagggc gaggccgtcg ccgtgggcga actcgacatc accatgtacc
     1981 gcgacgatct gcgccgccag cccacccggg cggtcggacg cacctcgatc ccccgttcca
     2041 tcgacgacac cacggtggtg ctggtcgacg acgtgctctt caccggacgc accatcgccg
     2101 ccgccctgga cgccctgaag gaggtgggcc gcccccgcat ggtgcaactc gccgtgctga
     2161 tcgaccgggg gcatcgtcaa ctgccgatcc aggccgacca tgtggggcgg ctgctgcagg
     2221 tgcccgccga ggcgcgggtc aacgtccgca tggccgagat cgacgaagtc gacgcggtca
     2281 cgatcagctt gccggaggcc tgatgcgaca cctgctgagc gccggcgatc taagcctggc
     2341 cgaggtcacg tccattctcg acgtcgccga agagatgcac accattcagc agcggcagat
     2401 caagaagctg cccaccctgc gcggtcgcac cgtgatcaac atgttcttcg aggattcgac
     2461 ccgcacccgc accagcttcg agatggccgg caaatggatg tcggccgaca cgatcaacat
     2521 ctcggccaag gggtcgtcca cctcgaaggg cgagtcgctg cgcgacacca tgctcaccct
     2581 ggacgccatg ggcaccgact gcttcgtgat ccggcacgga tcctcaggcg ccgtcgcaca
     2641 ggccgcttcg tgggtgcgcg cgcatgtggt gaacgccggc gacggcaccc atgagcaccc
     2701 cacccaggcg ctgctggacg ctttcgcgat gcgccgccac ttcggcaccc tcggcgcgtc
     2761 cgacagcggc ggccaggagg ccttcgccgg caagcggatc gcgatcgtcg gcgacctgct
     2821 gcacagccgg gtggtgcgca gcaacgtgct gctgcagcgc accctcggta gctccgtcac
     2881 gctggtggct ccgcccactc tgctgccgac cggcgtcgag cagtgggggg tgggcgtgac
     2941 cagcgacctc gacgaggtga tcgaaagcgt cgatgtggtg atgatgctgc gagtacagcg
     3001 cgaacgcatg cacggcgggt tctttcccac cgagcgcgag tacaccgagc tgtacgggct
     3061 gacccccgaa cgcctggccc ggctcaggcc cggcgcggcg atctg
//
LOCUS       DAOSUG010000383         3097 bp    DNA     linear   ENV 10-JUN-2024
DEFINITION  MAG TPA_asm: Micropruina sp. isolate AMR_MDS_2195
            NODE_12806_length_3097_cov_4.225510, whole genome shotgun sequence.
ACCESSION   DAOSUG010000383 DAOSUG010000000
VERSION     DAOSUG010000383.1
DBLINK      BioProject: PRJNA925477
            BioSample: SAMN32864262
            Sequence Read Archive: SRR4236673
KEYWORDS    WGS; ENV; Metagenome Assembled Genome; MAG; Third Party Data; TPA;
            TPA:assembly.
SOURCE      Micropruina sp. (wastewater metagenome)
  ORGANISM  Micropruina sp.
            Bacteria; Bacillati; Actinomycetota; Actinomycetes;
            Propionibacteriales; Nocardioidaceae; Micropruina.
REFERENCE   1  (bases 1 to 3097)
  AUTHORS   Abdulkadir,N., Saraiva,J.P., Zhang,J., Stolte,S., Gillor,O.,
            Harms,H. and Rocha,U.
  TITLE     Genome-centric analyses of 165 metagenomes show that mobile genetic
            elements are crucial for the transmission of antimicrobial
            resistance genes to pathogens in activated sludge and wastewater
  JOURNAL   Microbiol Spectr 12 (3), e0291823 (2024)
   PUBMED   38289113
REFERENCE   2  (bases 1 to 3097)
  AUTHORS   Abdulkadir,N.
  TITLE     Direct Submission
  JOURNAL   Submitted (24-MAR-2023) Environmental Microbiology, Helmholtz
            Center for Environmental Research -UFZ, Permoserstr 15, Leipzig
            04318, Germany
COMMENT     The annotation was added by the NCBI Prokaryotic Genome Annotation
            Pipeline (PGAP). Information about PGAP can be found here:
            https://www.ncbi.nlm.nih.gov/genome/annotation_prok/
            
            ##Genome-Assembly-Data-START##
            Assembly Method        :: SPAdes v. 3.11.1
            Genome Representation  :: Full
            Expected Final Version :: Yes
            Genome Coverage        :: Not Applicable
            Sequencing Technology  :: Illumina MiSeq
            ##Genome-Assembly-Data-END##
            
            ##Genome-Annotation-Data-START##
            Annotation Provider               :: NCBI
            Annotation Date                   :: 05/04/2023 04:57:10
            Annotation Pipeline               :: NCBI Prokaryotic Genome
                                                 Annotation Pipeline (PGAP)
            Annotation Method                 :: Best-placed reference protein
                                                 set; GeneMarkS-2+
            Annotation Software revision      :: 6.5
            Features Annotated                :: Gene; CDS; rRNA; tRNA; ncRNA
            Genes (total)                     :: 2,776
            CDSs (total)                      :: 2,750
            Genes (coding)                    :: 2,714
            CDSs (with protein)               :: 2,714
            Genes (RNA)                       :: 26
            tRNAs                             :: 24
            ncRNAs                            :: 2
            Pseudo Genes (total)              :: 36
            CDSs (without protein)            :: 36
            Pseudo Genes (ambiguous residues) :: 0 of 36
            Pseudo Genes (frameshifted)       :: 2 of 36
            Pseudo Genes (incomplete)         :: 32 of 36
            Pseudo Genes (internal stop)      :: 2 of 36
            ##Genome-Annotation-Data-END##
FEATURES             Location/Qualifiers
     source          1..3097
                     /organism="Micropruina sp."
                     /mol_type="genomic DNA"
                     /submitter_seqid="NODE_12806_length_3097_cov_4.225510"
                     /isolate="AMR_MDS_2195"
                     /isolation_source="Activated sludge of municipal
                     wastewater treatment plants"
                     /db_xref="taxon:2737536"
                     /environmental_sample
                     /geo_loc_name="Canada"
                     /lat_lon="49.25 N 123.1 W"
                     /collection_date="2015-06-16"
                     /metagenome_source="wastewater metagenome"
                     /note="metagenomic"
     gene            <1..717
                     /locus_tag="PLF56_11435"
     CDS             <1..717
                     /locus_tag="PLF56_11435"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_013863380.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="dipeptidyl aminopeptidase"
                     /protein_id="HOQ54210.1"
                     /translation="LARGWNAFVYDGPGQQSMLFDHGTHFRPDWEAVLTPVLDGLLAR
                     PDVRADQLAGYGLSQAGYWLPRALAFEHRLAAAVVDPGVVDVSTSWTEHLSKGMIRQL
                     RAGDRKPFNQAMALATHLPGLARTLRYRSRPYRHTDWFDLYRTVSEYRLEPALAARIE
                     TPLLITDPQGEQFWPGQSTTLARLVPDAELAHFTAAQGADLHCQPLGRLLTESRVFDW
                     LEERLGLSESVHPGAAAGTR"
     gene            complement(674..>3097)
                     /locus_tag="PLF56_11440"
     CDS             complement(674..>3097)
                     /locus_tag="PLF56_11440"
                     /inference="COORDINATES: protein motif:HMM:NF012422.2"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_process: GO:0006355 - regulation of DNA-templated
                     transcription [Evidence IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="LuxR C-terminal-related transcriptional
                     regulator"
                     /protein_id="HOQ54211.1"
                     /translation="FAHPPAAVAARAFAQWIDEHTRWGPLLLCLDDAQWADEQSMIAI
                     GFVLRRLDGAAVLVALGQRSGVGGLTLHELTVSGSRAHRIVLSGLSLEETSDLVRARR
                     PDVTQGTILDLWQHTGGNPLYLTSLLDELSAVELEELSDRWPVPRAFSSSVGRRLTRL
                     SAEARAMVETIAVLSRGYSALPDIVTIAGCASPHEALQELVDADLVKVSRSGLLAARV
                     AHALTRASVLELIPLPRRRELHRLAATVSSGQEALDHRLAASTVTDASLATELAQRAA
                     ALHADGAYRQAAHYYRAAADITPEPGQRRSRLFECRWDAALAGSRRAFDGPRPEFAAE
                     TVTVALTHFRGGDIGPAMALLEELSDAHVAAAEPVVRHRAAILRAYLRMLTGQDTGRI
                     EEQIALADGAAAPDPALRGIESPTRGFVASRRSSGDAELVAAFASLPDRATTVPEGLL
                     GLLAWRAVYRVYALHVREAADDFETLISRQVRHRDVTEFRTQLALARWLAGDWPLARV
                     ATAVPSATSDPLRWAPDFGAALIDAGLGRFAEADRHLGVAMANARKAPWPEGRLMLLV
                     ARVVRVHSAGELSHEVAGLVADYRDVAELIEIVTRSADGLLLMHAGLLGLWAGRPQVT
                     RRCIEIMAGSALPAPSSPAVLAWLHGLLARQAGDARAARGWFERAAHDDRNELLLYRA
                     HMWADLAGVADDPRQAAEATAEALDGYRRLGAVPYVERLQAAAAAPSGVADEAATDVF
                     LPELTDRERDVLALLVKGLSYTQIAGELFVTRSAVAFHLSNIYAKFDVRSRHDLTAYL
                     LRHPGALTR"
BASE COUNT          463 a         1136 c         1079 g          419 t
ORIGIN      
        1 ctggcccgcg gctggaacgc gttcgtctac gacggccccg gacagcagtc gatgctgttc
       61 gaccacggca cccacttccg tccggactgg gaggcggtgc tgacgcccgt gctggacggt
      121 ctgctggccc gtccggacgt ccgcgccgat cagttggccg gctacgggct cagccaggcc
      181 ggctactggc ttccgcgcgc cctggccttc gagcatcggc tcgccgcggc cgtcgtcgac
      241 ccgggcgtgg tggacgtctc gacgtcatgg accgaacacc tcagcaaggg catgatccgc
      301 caactccggg ccggcgaccg caagccgttc aaccaggcga tggcgctggc cacccacctg
      361 ccgggactgg ccaggaccct gcggtaccgg tcccggccgt accggcacac cgactggttc
      421 gacctctatc gcacggtgtc cgagtaccgt ctcgagccgg ccctcgccgc ccggatcgag
      481 accccgctgc tgatcactga cccgcagggc gagcagttct ggccgggcca gtccacgacc
      541 ctggcacgcc tggtacccga cgccgagctg gcgcacttca cggcggccca gggggccgac
      601 ctgcactgcc agccgctggg caggctgctc accgagagcc gcgtgttcga ctggctggag
      661 gagcggctgg gcctcagcga gtcagtgcac ccgggtgccg cagcaggtac gcggtgaggt
      721 cgtgccgcga acgcacgtcg aacttggcgt agatgttcga caggtgaaag gcgaccgcgc
      781 tgcgggtgac gaacagctcg ccggcgatct gggtgtagct gagtcccttg accagcaggg
      841 ccagcacatc ccgctcccga tcggtgagct ccggtaggaa cacgtcggtc gccgcctcgt
      901 ccgccacgcc ggacggcgcg gctgccgcgg cctgcagccg ctcgacgtac ggcaccgccc
      961 ccagcctgcg gtagccgtcc agggcctcgg cggtcgcctc ggcggcctgc cgaggatcgt
     1021 ccgccactcc ggccaggtcg gcccacatgt gcgcccggta gagcagcaac tcgtttcggt
     1081 cgtcgtgggc tgcgcgctcg aaccacccgc gggcggcccg ggcatcgccc gcctgccggg
     1141 ccagcagccc gtgcagccag gccagcacgg ccgggctgga cggcgcgggc agggcgctgc
     1201 ccgccatgat ctcgatgcag cggcgggtga cctgcggtcg tccggcccac aatccgagca
     1261 gtccggcgtg catcagcaac aacccgtccg cggacctggt cacgatctca atcagttcgg
     1321 ccacgtcgcg gtagtcggcg accagtccgg ccacctcatg cgagagttcg ccggcgctgt
     1381 ggacgcgcac cacccgggcg accagcagca tcagccgacc ttctggccac ggagccttcc
     1441 gggcattggc catggcgaca ccgaggtgcc ggtccgcctc cgcgaagcgg ccgagcccag
     1501 catcgatcag tgccgcaccg aagtccggcg cccagcgcag cggatcgctc gtcgcggacg
     1561 ggacggcggt cgccacccgg gccagcggcc agtccccggc gagccagcgg gccagggcca
     1621 gctgggtacg gaactcggtg acgtcgcggt gccggacctg cctgctgatc agcgtctcga
     1681 agtcgtccgc cgcctcgcgg acgtgcagcg catagacacg gtagacggcc cgccaggcca
     1741 gcaggccgag gaggccctcg gggaccgtcg tggcccggtc aggcaacgac gcgaaggcgg
     1801 cgacgagttc cgcgtcgcca ctggagcgtc gactggcgac gaaacccctt gtcggggact
     1861 cgatcccccg cagggccggg tccggggcag cggccccgtc ggccagcgcg atctgctcct
     1921 cgatccgtcc ggtgtcctgt ccggtcagca tccgcagata ggcgcgcagg atcgccgcgc
     1981 ggtgccggac gaccggctcg gcggcagcga cgtgtgcgtc ggacagctcc tccagcaacg
     2041 ccatggccgg cccgatgtct ccgccgcgga agtgggtcag tgcgacggtg accgtctcgg
     2101 ccgcgaactc gggccggggc ccatcgaacg cccgccgcga acctgccagg gccgcatccc
     2161 accggcactc gaacagccgg ctccggcgtt ggccgggctc gggcgtgatg tccgctgctg
     2221 cccggtagta gtgcgccgcc tggcggtagg cgccgtcggc gtgaagcgca gcagcccgtt
     2281 gggccagctc tgtggccaac gaggcgtcgg tgacggtgct cgccgcgagc cggtgatcca
     2341 gggcctcctg cccgctgctc accgtggccg ccaaccggtg cagttcgcgc ctgcgaggca
     2401 gcgggatcag ttccagcacg gatgccctgg tcagtgcgtg agcgacccga gccgccagaa
     2461 ggccggagcg gctgaccttc accaggtcgg cgtccaccag ttcctggagc gcctcatgcg
     2521 ggctcgcgca gccggcgatc gtgacgatgt cgggcagcgc cgagtagccg cgactcagca
     2581 ccgcgatcgt ctcgaccatg gcgcgagcct cggcactcaa ccgcgtgagc cgacgcccca
     2641 ccgacgacga gaacgcgcgc gggacgggcc agcgatcaga cagctcctcc agttcgacgg
     2701 cactcagctc gtccagcagc gacgtcaggt acagcgggtt tcctccggtg tgctgccaca
     2761 ggtccaggat cgtcccctgg gtgacatcgg gacgccgggc tcggacgagg tcggacgtct
     2821 cctcgagaga caacccggac agcacgatcc ggtgcgcgcg tgaaccagac actgtcaact
     2881 cgtgcagcgt cagccctccg acgccagaac gctgcccgag tgcgaccagc accgcggcgc
     2941 cgtccagccg tcgaagcacg aagccgatcg caatcatcga ctgttcgtca gcccactggg
     3001 catcgtcgag gcacaggagc agcggtcccc accgggtgtg ttcatcgatc cattgggcga
     3061 acgcacgcgc ggccacggcg gcggggggat gtgcgaa
//
LOCUS       DAOSUG010000384         3095 bp    DNA     linear   ENV 10-JUN-2024
DEFINITION  MAG TPA_asm: Micropruina sp. isolate AMR_MDS_2195
            NODE_12819_length_3095_cov_4.011513, whole genome shotgun sequence.
ACCESSION   DAOSUG010000384 DAOSUG010000000
VERSION     DAOSUG010000384.1
DBLINK      BioProject: PRJNA925477
            BioSample: SAMN32864262
            Sequence Read Archive: SRR4236673
KEYWORDS    WGS; ENV; Metagenome Assembled Genome; MAG; Third Party Data; TPA;
            TPA:assembly.
SOURCE      Micropruina sp. (wastewater metagenome)
  ORGANISM  Micropruina sp.
            Bacteria; Bacillati; Actinomycetota; Actinomycetes;
            Propionibacteriales; Nocardioidaceae; Micropruina.
REFERENCE   1  (bases 1 to 3095)
  AUTHORS   Abdulkadir,N., Saraiva,J.P., Zhang,J., Stolte,S., Gillor,O.,
            Harms,H. and Rocha,U.
  TITLE     Genome-centric analyses of 165 metagenomes show that mobile genetic
            elements are crucial for the transmission of antimicrobial
            resistance genes to pathogens in activated sludge and wastewater
  JOURNAL   Microbiol Spectr 12 (3), e0291823 (2024)
   PUBMED   38289113
REFERENCE   2  (bases 1 to 3095)
  AUTHORS   Abdulkadir,N.
  TITLE     Direct Submission
  JOURNAL   Submitted (24-MAR-2023) Environmental Microbiology, Helmholtz
            Center for Environmental Research -UFZ, Permoserstr 15, Leipzig
            04318, Germany
COMMENT     The annotation was added by the NCBI Prokaryotic Genome Annotation
            Pipeline (PGAP). Information about PGAP can be found here:
            https://www.ncbi.nlm.nih.gov/genome/annotation_prok/
            
            ##Genome-Assembly-Data-START##
            Assembly Method        :: SPAdes v. 3.11.1
            Genome Representation  :: Full
            Expected Final Version :: Yes
            Genome Coverage        :: Not Applicable
            Sequencing Technology  :: Illumina MiSeq
            ##Genome-Assembly-Data-END##
            
            ##Genome-Annotation-Data-START##
            Annotation Provider               :: NCBI
            Annotation Date                   :: 05/04/2023 04:57:10
            Annotation Pipeline               :: NCBI Prokaryotic Genome
                                                 Annotation Pipeline (PGAP)
            Annotation Method                 :: Best-placed reference protein
                                                 set; GeneMarkS-2+
            Annotation Software revision      :: 6.5
            Features Annotated                :: Gene; CDS; rRNA; tRNA; ncRNA
            Genes (total)                     :: 2,776
            CDSs (total)                      :: 2,750
            Genes (coding)                    :: 2,714
            CDSs (with protein)               :: 2,714
            Genes (RNA)                       :: 26
            tRNAs                             :: 24
            ncRNAs                            :: 2
            Pseudo Genes (total)              :: 36
            CDSs (without protein)            :: 36
            Pseudo Genes (ambiguous residues) :: 0 of 36
            Pseudo Genes (frameshifted)       :: 2 of 36
            Pseudo Genes (incomplete)         :: 32 of 36
            Pseudo Genes (internal stop)      :: 2 of 36
            ##Genome-Annotation-Data-END##
FEATURES             Location/Qualifiers
     source          1..3095
                     /organism="Micropruina sp."
                     /mol_type="genomic DNA"
                     /submitter_seqid="NODE_12819_length_3095_cov_4.011513"
                     /isolate="AMR_MDS_2195"
                     /isolation_source="Activated sludge of municipal
                     wastewater treatment plants"
                     /db_xref="taxon:2737536"
                     /environmental_sample
                     /geo_loc_name="Canada"
                     /lat_lon="49.25 N 123.1 W"
                     /collection_date="2015-06-16"
                     /metagenome_source="wastewater metagenome"
                     /note="metagenomic"
     gene            <1..1508
                     /locus_tag="PLF56_11445"
     CDS             <1..1508
                     /locus_tag="PLF56_11445"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_015069369.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=3
                     /transl_table=11
                     /product="DUF5915 domain-containing protein"
                     /protein_id="HOQ54212.1"
                     /translation="VGTMVFDQSSYRNVLCLGHILAEDGRKMSKHLGNILEPIPLMDT
                     HGADAVRWFMAAGGSPWAARRVGHSTIIETVRKVLLTYWNSVAFQALYARTNGWTPAA
                     PSLELVETEASVIERVETGTPSVEVVETENVRVEFIETPSQPAHVLDRWLVSVRTTLV
                     REVTAALEAFDTQRYGTLIADFVDDLSNWYVRRSRRRFWEGDAGALQTLHDTLNVVTR
                     LMAPLAPFITERVWQDLMVATDPAASESVHLASWPVVDESLADPELDASMQLTRRLVE
                     LGRSARAEARVKTRQPLSRVLVPSGSFARLTEELRAEIAAELNVQRVESFASAGDLVD
                     YSAKGNFRALGKRFGKETPKVAAAIAAADAAALAAALAADGVATVGGVEVSADEVIVS
                     ERPREGWSVVNEQGETVALDLELTPELLQAGLAREVVRLVQERRKNSGLEVSDRITLA
                     WAATGDLAEAIRAHAALIASEVLATTVVEDEPGDEWATDDDLGLTFSISKV"
     gene            complement(1710..2069)
                     /locus_tag="PLF56_11450"
     CDS             complement(1710..2069)
                     /locus_tag="PLF56_11450"
                     /inference="COORDINATES: ab initio
                     prediction:GeneMarkS-2+"
                     /note="Derived by automated computational analysis using
                     gene prediction method: GeneMarkS-2+."
                     /codon_start=1
                     /transl_table=11
                     /product="hypothetical protein"
                     /protein_id="HOQ54213.1"
                     /translation="MTVNPPTGEQEPRWQQPDFQPTQSEPQFFTPSPNQAMVPSYPAP
                     VLPPPGTFETVVGTLARLVWPVAILLYFFTPVTFWPALVGAIIAGTVLGAVKKNLKQQ
                     RRASVRYVQQTPGSDQR"
     gene            2314..2553
                     /locus_tag="PLF56_11455"
     CDS             2314..2553
                     /locus_tag="PLF56_11455"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_014001300.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="hypothetical protein"
                     /protein_id="HOQ54214.1"
                     /translation="MRTTLDLDDAVLAAARSLAKAERLSLGAAVSRLARRGLGLDATA
                     SVTADVIHSGPFAVLIGDPEHLVTPDLVEQFRDGD"
     gene            2540..2992
                     /locus_tag="PLF56_11460"
     CDS             2540..2992
                     /locus_tag="PLF56_11460"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_013861040.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="PIN domain nuclease"
                     /protein_id="HOQ54215.1"
                     /translation="MTATDSGQPGWLPDVNVLVALTLTTHVHHARAHESLRRHRGDWA
                     TSPLTEAALVRLLLNPTVTGRTFAPALVLQVVSGLRTDPRWRWLPDDSTLADPVIDLS
                     VLVGHRQVTDLHLVNLAASAGLRLVTFDAGIAAALAKADRRHVLVLPQ"
BASE COUNT          455 a         1000 c         1121 g          519 t
ORIGIN      
        1 cggtcggcac gatggtgttc gaccagtcca gctaccgcaa cgtgctgtgc ctgggccaca
       61 tcctcgccga ggacggccgc aagatgagca agcacctggg caacatcctg gagccgatcc
      121 cgctgatgga cacccacggt gcggacgcgg tgcgctggtt catggcggcc ggcggttcgc
      181 cgtgggcggc tcgccgagtg gggcactcca cgatcatcga gaccgtccgc aaggtgctgc
      241 tgacctactg gaactcggtc gccttccagg ccctctacgc gcggacgaac ggctggacgc
      301 ccgccgcacc gtcgctcgag cttgtcgaaa ccgaggcttc ggtgatcgag cgtgtcgaga
      361 ccgggacccc gtcggtcgag gttgtcgaga ccgagaatgt gcgggtcgag ttcatcgaga
      421 ccccctccca gcccgcccac gtgctcgacc gctggctggt ctctgtgcgg accacgctgg
      481 tgcgcgaggt cacggcggcg ctggaggcct tcgacaccca gcggtacggc acgctgatcg
      541 ccgacttcgt ggacgacctg tccaactggt acgtgcgccg gtcgcgtcgc cgcttctggg
      601 agggcgacgc gggcgcgctg cagaccctgc acgacacgct gaatgtggtc acccggctga
      661 tggcgccgct ggccccgttc atcaccgagc gggtctggca ggacctgatg gtggcgacgg
      721 atcctgctgc gtccgagtcg gtgcacctgg cgtcctggcc cgtggtggac gagtcgctgg
      781 ccgatccgga actggacgcc tcgatgcaac tgacccgtcg cctcgtcgaa ctcggacggt
      841 ccgcacgcgc cgaggccagg gtgaagaccc gccagccgtt gtcgcgggta ctggtgccgt
      901 cggggtcgtt cgcgaggctg actgaggagt tgcgggccga gatcgccgcc gaactcaacg
      961 tgcagcgggt cgaatcgttc gcctcggccg gcgacctggt cgactactcg gccaagggca
     1021 acttccgcgc cctgggcaag cgattcggca aagagacacc caaggtcgcc gccgccatcg
     1081 ccgccgcgga cgctgctgcg ctggccgccg ctctggcggc cgacggggtg gccaccgtgg
     1141 ggggcgtcga ggtgagcgcg gacgaggtga tcgtctcgga gcgtccgcgt gagggttggt
     1201 cggtggtcaa cgaacagggc gaaaccgtgg cgctcgattt ggagttgacg cccgagttgc
     1261 tgcaggcggg gttggcgcgc gaggtcgtcc ggctggtgca ggaacggcgc aagaactcgg
     1321 gccttgaggt ctcggaccgg atcaccctgg cgtgggccgc cacgggtgac ctggctgagg
     1381 cgatccgcgc ccatgcggcc ctgatcgcgt ccgaggtgct ggccacgacg gtcgtcgagg
     1441 acgagcctgg tgacgagtgg gcgacggacg acgacctggg cctcacgttc tcgatctcga
     1501 aggtctagtg gtgtgtcccc caagtggctt gctgtttggc ggtgtctgag tgcgtgcatc
     1561 gcaaggccga cgagggaatc gataccgggt cgtatcgcga ccgaggagaa cgccgcgagg
     1621 tgcgtgctca ggcgcagcca accggtgagt gacttggggg acacaccact aggtccggtg
     1681 ccgccctgat ccgtcgacga gggacgcgat cagcgctggt cggagccggg tgtctgctgc
     1741 acgtaacgga cgctcgcgcg gcgttgctgc ttgaggttct tcttcaccgc gccgagaacg
     1801 gtgccggcga tgatcgcgcc gaccagggcc ggccagaagg tcaccggtgt gaagaagtac
     1861 agcagaatcg caaccggcca gaccaggcgg gccagggtgc cgacgacggt ctcgaaggtg
     1921 ccgggtggcg gcaggacggg cgctgggtac gacggcacca tcgcctgatt cggtgacggc
     1981 gtgaagaact gcggctcgct ctgcgtcggc tgaaagtccg gttgttgcca gcgcggctcc
     2041 tgctcaccgg tcggcggatt gacggtcact tggtccatcg tagtgaggac tcgcccatcc
     2101 gcaccgagac ccagcgagtg tggcgccatg cccacgacct cgtccaagtc ctgttgcccg
     2161 acgttgcagg gctcaaggaa acgttgtcag ggcccgaacc gttggtcgag cctgtcggga
     2221 ccccgtggtc gcgtgggact ggcaggcgcg agcatcgatg gatcgatgcg ttcgcgggct
     2281 cgctcgaaac agctgtgatg ctatgatgcg gtgatgcgca ccacgttgga tctggacgac
     2341 gcggtccttg ccgctgcgcg ctcgctggca aaggctgaac ggctgtcgtt gggtgccgcg
     2401 gtgtcgcggc tggcccggcg cgggctgggt ctggatgcca cagcgagcgt gacggccgat
     2461 gtgatccact cgggcccttt tgcggtgctg atcggcgacc cggaacacct cgtcacgccc
     2521 gatctggtcg agcagtttcg tgacggcgac tgactccggg cagccgggtt ggctgcccga
     2581 cgtcaacgtg ctggtcgccc tcaccctgac cacccatgtg caccacgccc gggcgcacga
     2641 gtcgttgcgc cgccatcgcg gcgactgggc gaccagtccg ctcaccgagg ctgcgttggt
     2701 cagattgctg ctcaatccga cggtgacggg ccgtacgttc gcaccggcac tggtgctgca
     2761 ggtcgtgtcg ggcctgcgca ccgacccgcg ctggcgctgg ctgcccgatg attcaacgct
     2821 ggccgacccg gtgatcgacc tctcggttct ggtcgggcac cggcaggtca ccgatctgca
     2881 cctggtgaac ctcgcggcaa gcgcggggct gcggctggtc accttcgatg cgggcattgc
     2941 cgctgccctg gccaaggccg accggcgcca cgtgctggtg ttgccgcagt agcgccggtc
     3001 gtacctccgt cgatgccgcc gagacggtgc cgccgagacg gtgccgccga gacggtgccg
     3061 ctcagtcggt gaccgtcagc acggcggtca gctcg
//
LOCUS       DAOSUG010000385         3082 bp    DNA     linear   ENV 10-JUN-2024
DEFINITION  MAG TPA_asm: Micropruina sp. isolate AMR_MDS_2195
            NODE_12884_length_3082_cov_4.230922, whole genome shotgun sequence.
ACCESSION   DAOSUG010000385 DAOSUG010000000
VERSION     DAOSUG010000385.1
DBLINK      BioProject: PRJNA925477
            BioSample: SAMN32864262
            Sequence Read Archive: SRR4236673
KEYWORDS    WGS; ENV; Metagenome Assembled Genome; MAG; Third Party Data; TPA;
            TPA:assembly.
SOURCE      Micropruina sp. (wastewater metagenome)
  ORGANISM  Micropruina sp.
            Bacteria; Bacillati; Actinomycetota; Actinomycetes;
            Propionibacteriales; Nocardioidaceae; Micropruina.
REFERENCE   1  (bases 1 to 3082)
  AUTHORS   Abdulkadir,N., Saraiva,J.P., Zhang,J., Stolte,S., Gillor,O.,
            Harms,H. and Rocha,U.
  TITLE     Genome-centric analyses of 165 metagenomes show that mobile genetic
            elements are crucial for the transmission of antimicrobial
            resistance genes to pathogens in activated sludge and wastewater
  JOURNAL   Microbiol Spectr 12 (3), e0291823 (2024)
   PUBMED   38289113
REFERENCE   2  (bases 1 to 3082)
  AUTHORS   Abdulkadir,N.
  TITLE     Direct Submission
  JOURNAL   Submitted (24-MAR-2023) Environmental Microbiology, Helmholtz
            Center for Environmental Research -UFZ, Permoserstr 15, Leipzig
            04318, Germany
COMMENT     The annotation was added by the NCBI Prokaryotic Genome Annotation
            Pipeline (PGAP). Information about PGAP can be found here:
            https://www.ncbi.nlm.nih.gov/genome/annotation_prok/
            
            ##Genome-Assembly-Data-START##
            Assembly Method        :: SPAdes v. 3.11.1
            Genome Representation  :: Full
            Expected Final Version :: Yes
            Genome Coverage        :: Not Applicable
            Sequencing Technology  :: Illumina MiSeq
            ##Genome-Assembly-Data-END##
            
            ##Genome-Annotation-Data-START##
            Annotation Provider               :: NCBI
            Annotation Date                   :: 05/04/2023 04:57:10
            Annotation Pipeline               :: NCBI Prokaryotic Genome
                                                 Annotation Pipeline (PGAP)
            Annotation Method                 :: Best-placed reference protein
                                                 set; GeneMarkS-2+
            Annotation Software revision      :: 6.5
            Features Annotated                :: Gene; CDS; rRNA; tRNA; ncRNA
            Genes (total)                     :: 2,776
            CDSs (total)                      :: 2,750
            Genes (coding)                    :: 2,714
            CDSs (with protein)               :: 2,714
            Genes (RNA)                       :: 26
            tRNAs                             :: 24
            ncRNAs                            :: 2
            Pseudo Genes (total)              :: 36
            CDSs (without protein)            :: 36
            Pseudo Genes (ambiguous residues) :: 0 of 36
            Pseudo Genes (frameshifted)       :: 2 of 36
            Pseudo Genes (incomplete)         :: 32 of 36
            Pseudo Genes (internal stop)      :: 2 of 36
            ##Genome-Annotation-Data-END##
FEATURES             Location/Qualifiers
     source          1..3082
                     /organism="Micropruina sp."
                     /mol_type="genomic DNA"
                     /submitter_seqid="NODE_12884_length_3082_cov_4.230922"
                     /isolate="AMR_MDS_2195"
                     /isolation_source="Activated sludge of municipal
                     wastewater treatment plants"
                     /db_xref="taxon:2737536"
                     /environmental_sample
                     /geo_loc_name="Canada"
                     /lat_lon="49.25 N 123.1 W"
                     /collection_date="2015-06-16"
                     /metagenome_source="wastewater metagenome"
                     /note="metagenomic"
     gene            complement(325..1296)
                     /locus_tag="PLF56_11465"
     CDS             complement(325..1296)
                     /locus_tag="PLF56_11465"
                     /EC_number="1.1.1.-"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_020387695.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_function: GO:0016616 - oxidoreductase activity, acting
                     on the CH-OH group of donors, NAD or NADP as acceptor
                     [Evidence IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="aldo/keto reductase"
                     /protein_id="HOQ54216.1"
                     /translation="MKHTTLGTLDVSRLGLGTMGMSAFYSGAGTDDAESTRTIRRAID
                     LGVTFFDTAEMYGPYINEELLGRALGGDRDSVVVATKGGTLKHLDDDSYGFDGRPANL
                     RIALEGSLKRLGTDHVDLYYQHRMDPAVPIEETVGAMAEFIAEGKILGYGLSEAAPER
                     IRAAHAVHPITAIQTEYSLWSRDPEAELLPLLRELGVGFVPYSPLGRGFLTGALRSID
                     QLDAQDFRRNNPRFQGENLKQNLAIVDAVEVVAAEADATSAQVALAWIVAQGEGIAPI
                     PGTKRVGRLEENVAADQLTLSDDQLALLDALPKPSGDRYADMSSVNR"
     gene            complement(1293..1673)
                     /locus_tag="PLF56_11470"
     CDS             complement(1293..1673)
                     /locus_tag="PLF56_11470"
                     /inference="COORDINATES: protein motif:HMM:NF024803.2"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_function: GO:0003677 - DNA binding [Evidence IEA];
                     GO_process: GO:0006355 - regulation of DNA-templated
                     transcription [Evidence IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="MerR family transcriptional regulator"
                     /protein_id="HOQ54217.1"
                     /translation="MTTTEPELTIAEMAAETGLTTSTLRYYEQEGLLLQAPGRAPSSH
                     RRYPSSAINWLIFVTRLRSTGMPVRDIRRYAALARQGEHTTSERLQLLIEHRARVRAQ
                     LEQLQHSLSAIDYKIASYERFTTR"
     gene            1874..2353
                     /locus_tag="PLF56_11475"
     CDS             1874..2353
                     /locus_tag="PLF56_11475"
                     /inference="COORDINATES: protein motif:HMM:NF027567.2"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="pyridoxamine 5'-phosphate oxidase family
                     protein"
                     /protein_id="HOQ54218.1"
                     /translation="MSVSDDQRETYERLLGKSRTVMLTTVSAEGKLVSRPMTIQEVTD
                     DQQLVFITQRNTDVARESEGHEVNVAVVGDDYWISISGTATIGDEPETKKRLWNSFND
                     AYTEGGPENPDNVVLRVSADSATYWDTPGGLGVLLGVLKAKVTGGKPESGDSATVEL"
     gene            complement(2597..>3082)
                     /locus_tag="PLF56_11480"
     CDS             complement(2597..>3082)
                     /locus_tag="PLF56_11480"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_020574874.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_component: GO:0016020 - membrane [Evidence IEA];
                     GO_function: GO:0022857 - transmembrane transporter
                     activity [Evidence IEA];
                     GO_process: GO:0055085 - transmembrane transport [Evidence
                     IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="efflux RND transporter permease subunit"
                     /protein_id="HOQ54219.1"
                     /translation="LLVSIPFAATGALGLSLLTDTPLGVASMIGLLMLIGIVVTNAIV
                     LIDLINQKRRAGAGVDESIAQGARLRVRPIVMTALATIFALVPMGLGLTGGGVFISKP
                     LAIVVIGGLVSSTLLTLILVPVLYDLLETWRQNRRDRTAARKAAKQAPPADQDGVAAT
                     S"
BASE COUNT          510 a         1036 c         1060 g          476 t
ORIGIN      
        1 cggtcgatgt cgccgacctg agcgccacgg gcgaggccac ccacctcggc tggtggtggg
       61 cagtcggctc gctggtgttg gcggtggtcg ccatcgcgtc ccaactgcgg tcactgaacc
      121 gttcaaaggc gacggcgatg cgcgcccagt gggcacagcc cgtccgctga gaccggacgg
      181 gcccacctcg acacgtccga tccaggggcg cacgagaggt tcgtcggtcg tgcgggtcgg
      241 ttccgcggcc ggctggaggt ggccggccgg cggaggcctc gacaggctcg gccagcgatc
      301 ggtgcgggtt cggttggggt ttgcctaccg gttcactgac gacatgtccg cgtaccgatc
      361 gcccgacggc ttcggcaggg cgtccagcaa cgccaactgg tcgtcgctga gcgtgagctg
      421 gtccgccgcc acgttctctt ccagccggcc gacccgcttg gtgccgggga tcggcgcgat
      481 gccctcaccc tgagcgacaa tccacgccag cgccacctgg gccgacgtgg catcggcctc
      541 ggcggcgacg acctcgacgg cgtccacgat ggcgaggttc tgcttcaggt tctcgccctg
      601 gaagcgcggg ttgttgcgcc ggaagtcctg ggcgtcgagt tggtcgatcg agcgcagcgc
      661 accggtcagg aacccgcggc ccagcggcga atacggtacg aaaccgacgc cgagctcgcg
      721 cagcagcgga agcagttctg cctcggggtc acgcgaccac aaggagtact ccgtctggat
      781 cgccgtgatc gggtgcacgg catgggcagc tctgatgcgt tcgggggcgg cctccgacaa
      841 cccatagccg agaatcttgc cctcggcgat gaactcggcc atcgcgccga cggtctcttc
      901 gatcggcacc gcgggatcca tgcggtgctg gtaatagagg tcgacgtgat cagtgccgag
      961 tcgcttcagt gagccttcga gggcgatccg caggttcgcc ggacgtccgt cgaacccgta
     1021 ggagtcgtcg tccagatgct tcagcgtgcc gcccttggtg gccaccacga ccgagtcgcg
     1081 gtcgccgccg agcgcccggc ccagcagctc ttcgttgatg tagggcccgt acatttcggc
     1141 ggtgtcgaag aacgtgacgc ccaggtcgat cgcgcgacga atggtgcgcg tcgattcggc
     1201 gtcatcggtg ccggcgcccg aatagaaggc cgacatgccc atggtgccca ggcccaggcg
     1261 cgagacgtcc agggtgccga gagttgtgtg cttcatctgg tggtgaacct ttcgtagctt
     1321 gcgatcttgt agtcgatggc gctcaggctg tgctgcaact gctcgagttg ggcacgcacc
     1381 cgcgcacgat gctcgatcag caactgcagc cgctcggacg tggtgtgctc gccctggcgg
     1441 gccagcgcgg catagcggcg gatgtcgcgc accggcatgc cggtggagcg caaccgggtg
     1501 acaaagatga gccagttgat ggccgagctc gggtagcggc gatggctgga cggggcgcgt
     1561 cccggcgcct ggagcagcag cccctcctgc tcgtagtagc gcagggtcga ggtggtcagt
     1621 ccggtctcgg cagccatctc ggcgatggtc agctcaggct cggtcgtggt catgaactca
     1681 ccgtagaact tcaagcgcac ttcaagtcaa ccgcggtcga ggcgctcgtg cggagcgcgc
     1741 ggatccgcct caccccttgg gccacaacgt tcgacaaacc atggacaccg gtgctcggcc
     1801 cggttagcgt cgagcaatga aagccggtgc gacaagcccg atcgaccggc accgtccatt
     1861 cgatgggagc accatgagcg tcagtgacga tcaacgcgaa acctacgaac gactgctcgg
     1921 caagtcgcgc accgtgatgc tcaccacggt gagcgccgaa ggcaagctgg tcagccgtcc
     1981 gatgaccatc caagaggtga cggacgacca gcagctcgtc ttcatcaccc agcgcaacac
     2041 cgacgtggca cgcgagagcg aggggcacga ggtcaacgtc gcggtggtgg gggacgacta
     2101 ctggatctcg atctcgggca ccgccacgat cggcgacgag cctgagacca agaagcggct
     2161 ctggaactcg ttcaacgatg cctacaccga gggcgggccc gagaaccccg acaatgtcgt
     2221 gctgcgggtc agcgccgaca gcgccaccta ttgggacacc cccggcgggc tcggcgtgct
     2281 gctgggcgtg ctcaaggcga aggtgaccgg cggcaagccc gagagcggtg acagcgcgac
     2341 cgtcgagttg taggcgggcc gccccgcggg gccggccgac ccgcataggg ccgacccgca
     2401 tgggccgacc cgcgtgggcc gacccgcgtg ggcctgccgc ctgcgcggac gaccccgcgt
     2461 ggatgacgtt gcgcggtcga gcgctcgtcg cgcgcggacg aacctgcgca acgaaccctt
     2521 gaccgcgcaa cgtcacgtgc tgattcggtc acatggtgcc ctgcccgctc cctgacccag
     2581 ccgaggggcg tccccctcag gacgtcgcgg cgacgccgtc ctgatcggcc gggggagcct
     2641 gcttcgccgc cttgcgcgca gcggtgcggt cgcgccggtt ctgccgccag gtttcgagca
     2701 ggtcgtacag caccggcacc agaatgagcg tcagcagggt ggacgacacc aggcccccga
     2761 tcaccacgat ggccagcggc ttgctgatga acacaccgcc accggtcagc cccagcccca
     2821 tcggcaccag cgcgaagatc gtggccagag ccgtcatcac gatcggacgc acccgcagcc
     2881 gcgcgccctg cgcgatggat tcgtccaccc cggccccggc tcgtcgcttc tggttgatca
     2941 ggtcgatcag cacgatcgcg ttggtcacca cgatgccgat cagcatcagc aggccgatca
     3001 tgctggccac acccaagggt gtgtcggtca gtagcgacag ccccagcgcg cccgttgcgg
     3061 cgaacgggat cgacaccagc ag
//
LOCUS       DAOSUG010000386         3076 bp    DNA     linear   ENV 10-JUN-2024
DEFINITION  MAG TPA_asm: Micropruina sp. isolate AMR_MDS_2195
            NODE_12907_length_3076_cov_3.679245, whole genome shotgun sequence.
ACCESSION   DAOSUG010000386 DAOSUG010000000
VERSION     DAOSUG010000386.1
DBLINK      BioProject: PRJNA925477
            BioSample: SAMN32864262
            Sequence Read Archive: SRR4236673
KEYWORDS    WGS; ENV; Metagenome Assembled Genome; MAG; Third Party Data; TPA;
            TPA:assembly.
SOURCE      Micropruina sp. (wastewater metagenome)
  ORGANISM  Micropruina sp.
            Bacteria; Bacillati; Actinomycetota; Actinomycetes;
            Propionibacteriales; Nocardioidaceae; Micropruina.
REFERENCE   1  (bases 1 to 3076)
  AUTHORS   Abdulkadir,N., Saraiva,J.P., Zhang,J., Stolte,S., Gillor,O.,
            Harms,H. and Rocha,U.
  TITLE     Genome-centric analyses of 165 metagenomes show that mobile genetic
            elements are crucial for the transmission of antimicrobial
            resistance genes to pathogens in activated sludge and wastewater
  JOURNAL   Microbiol Spectr 12 (3), e0291823 (2024)
   PUBMED   38289113
REFERENCE   2  (bases 1 to 3076)
  AUTHORS   Abdulkadir,N.
  TITLE     Direct Submission
  JOURNAL   Submitted (24-MAR-2023) Environmental Microbiology, Helmholtz
            Center for Environmental Research -UFZ, Permoserstr 15, Leipzig
            04318, Germany
COMMENT     The annotation was added by the NCBI Prokaryotic Genome Annotation
            Pipeline (PGAP). Information about PGAP can be found here:
            https://www.ncbi.nlm.nih.gov/genome/annotation_prok/
            
            ##Genome-Assembly-Data-START##
            Assembly Method        :: SPAdes v. 3.11.1
            Genome Representation  :: Full
            Expected Final Version :: Yes
            Genome Coverage        :: Not Applicable
            Sequencing Technology  :: Illumina MiSeq
            ##Genome-Assembly-Data-END##
            
            ##Genome-Annotation-Data-START##
            Annotation Provider               :: NCBI
            Annotation Date                   :: 05/04/2023 04:57:10
            Annotation Pipeline               :: NCBI Prokaryotic Genome
                                                 Annotation Pipeline (PGAP)
            Annotation Method                 :: Best-placed reference protein
                                                 set; GeneMarkS-2+
            Annotation Software revision      :: 6.5
            Features Annotated                :: Gene; CDS; rRNA; tRNA; ncRNA
            Genes (total)                     :: 2,776
            CDSs (total)                      :: 2,750
            Genes (coding)                    :: 2,714
            CDSs (with protein)               :: 2,714
            Genes (RNA)                       :: 26
            tRNAs                             :: 24
            ncRNAs                            :: 2
            Pseudo Genes (total)              :: 36
            CDSs (without protein)            :: 36
            Pseudo Genes (ambiguous residues) :: 0 of 36
            Pseudo Genes (frameshifted)       :: 2 of 36
            Pseudo Genes (incomplete)         :: 32 of 36
            Pseudo Genes (internal stop)      :: 2 of 36
            ##Genome-Annotation-Data-END##
FEATURES             Location/Qualifiers
     source          1..3076
                     /organism="Micropruina sp."
                     /mol_type="genomic DNA"
                     /submitter_seqid="NODE_12907_length_3076_cov_3.679245"
                     /isolate="AMR_MDS_2195"
                     /isolation_source="Activated sludge of municipal
                     wastewater treatment plants"
                     /db_xref="taxon:2737536"
                     /environmental_sample
                     /geo_loc_name="Canada"
                     /lat_lon="49.25 N 123.1 W"
                     /collection_date="2015-06-16"
                     /metagenome_source="wastewater metagenome"
                     /note="metagenomic"
     gene            complement(<1..900)
                     /locus_tag="PLF56_11485"
     CDS             complement(<1..900)
                     /locus_tag="PLF56_11485"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_013863192.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_component: GO:0016020 - membrane [Evidence IEA];
                     GO_function: GO:0022857 - transmembrane transporter
                     activity [Evidence IEA];
                     GO_process: GO:0055085 - transmembrane transport [Evidence
                     IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="amino acid permease"
                     /protein_id="HOQ54220.1"
                     /translation="MNLFRTKSIEQSIADTDEPEYQLKKSLTALDLTVFGVGVVIGAG
                     IFTLTGRVAHQMTGPAIVISFVVAAVCCALAAMCYAEFASTVPVSGSAYTFSYASLGE
                     LFAWIIGWDLILEMFLGASVVAQGWSAYLGVLMEKLGLPIPAAIGYGGVVDLPAVLLI
                     VALGVLITIGIKESMRVNLVLVAVKLFIVLFVIVAGIMFINPANYTPFVPEAAPSTAA
                     AGFSWTQPLLQWLTGSAPMAFGWGGVFAGAALVFFAYIGFDVVATTAEEAKNPQKDLP
                     VGIIASLTICTILYCAVAVVVTGM"
     gene            1104..2234
                     /locus_tag="PLF56_11490"
     CDS             1104..2234
                     /locus_tag="PLF56_11490"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_013865581.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_function: GO:0005525 - GTP binding [Evidence IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="50S ribosome-binding GTPase"
                     /protein_id="HOQ54221.1"
                     /translation="MTQWFTDAFRTEFEKQAAQIGRFNLAVFGKTGVGKSTLINAIFG
                     EPVAATGIGDPVTLDTHLYLDTRGTLGLVDTRGIEIGRDDAELIKEVSKYVKQMRGKP
                     VSEQIHVAWYCVRGMDRRFEEVEGTFIRTLHDLGIPVLVVFTQVPMHDGQYHPDAVEL
                     ARLVEAKNLPIVGGRPFMTYAMRDQFTGQPPYGLMDVLQATFHVVPDAVHAALAAAQT
                     IDLQAKHQAAQRHINATVAAAAGAAAVPIPFSSAALLVPMQLGMMGRIAHLYQLPFDR
                     AALLAVASTSVATTLGRTAAANLLKFIPGAGSIAGGVINASVASGFTLAMGQSWLTVC
                     QRAAAGKLPTSVDGVLDSAAVKKLFEDEFRKRMPTIRRQDEK"
     gene            2364..2540
                     /locus_tag="PLF56_11495"
     CDS             2364..2540
                     /locus_tag="PLF56_11495"
                     /inference="COORDINATES: ab initio
                     prediction:GeneMarkS-2+"
                     /note="Derived by automated computational analysis using
                     gene prediction method: GeneMarkS-2+."
                     /codon_start=1
                     /transl_table=11
                     /product="hypothetical protein"
                     /protein_id="HOQ54222.1"
                     /translation="MGIARELVISPRTVDAPRSIFAELGIAASSDENARVLAVLSRLS
                     DPAAELVPGRGGPR"
     gene            2592..>3076
                     /locus_tag="PLF56_11500"
     CDS             2592..>3076
                     /locus_tag="PLF56_11500"
                     /inference="COORDINATES: protein motif:HMM:NF015286.2"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_function: GO:0003941 - L-serine ammonia-lyase activity
                     [Evidence IEA];
                     GO_function: GO:0051539 - 4 iron, 4 sulfur cluster binding
                     [Evidence IEA];
                     GO_process: GO:0006094 - gluconeogenesis [Evidence IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="serine dehydratase beta chain"
                     /protein_id="HOQ54223.1"
                     /translation="MALSFFDLFSIGIGPSSSHTTGPMRAARRVLDDLDAAGGLDRVW
                     RVRVDLYGSLGSTGRGHHSDRAVLLGLLGHRPETVDPAAVGPEVDRILARHRLSLLNR
                     HPIHFRPNRDLILHQRKSLPQHPNGMRVRCYSRDRKPIADRVLFSIGGGFVVDAADLA
                     DP"
BASE COUNT          524 a         1061 c         1000 g          491 t
ORIGIN      
        1 catgccggtg accaccacgg cgaccgcgca gtacagaatc gtgcagatgg tcagtgaggc
       61 gatgatgccg accggcaggt ccttctgcgg gttcttggcc tcttcggcgg tggtggccac
      121 cacgtcgaag ccgatgtagg cgaagaacac cagggccgct ccggcgaaca cgccgcccca
      181 gccgaacgcc atcggggccg agccggtcag ccattgcagc agcggctgcg tccatgagaa
      241 gcctgcggcg gccgtggacg gcgccgcttc gggaacgaat ggggtgtagt tggccggatt
      301 gatgaacatg atgcccgcca cgatcacgaa cagcacgatg aacagcttca cggccaccag
      361 caccaggttg acccgcatgg attccttgat gccgatggtg atcagcacac cgagcgccac
      421 gatcagcagc acggccggca ggtcgaccac gcccccatag ccgatggcgg ccggaatcgg
      481 caggcccagc ttctccatca gcacgccgag gtacgcgctc cagccctgcg ccaccacgct
      541 ggccccgagg aacatctcca gaatcaggtc ccagccgatg atccacgcga acagttcgcc
      601 gagtgaggcg tagctgaagg tgtaggccga gcctgagacc ggcaccgtcg aggcgaactc
      661 ggcgtaacac atggcggcca gtgcgcagca gaccgccgcc accacgaagc tgatcacgat
      721 ggccgggccg gtcatctgat gcgcgacgcg tccggtcagg gtgaagatgc cggcaccgat
      781 cacgacgccc acgccgaaga cggtcagatc gagtgcggtc agcgacttct tgagctggta
      841 ctccggctcg tcggtatcgg cgatcgattg ctcgatcgac ttggtgcgga acaggttcat
      901 cggttcctcc gggatttctt cgtcgaaacg gggtcgtcac aagctagtac ctgacaaccg
      961 ggcgaggatc ccgtctcgca aaaggctgaa cgccgatgtc gagcgtgccg caggcggcgc
     1021 gcactggttc caaggggtga atcggggcac cctgcgggtc ggcggcctcg tccggacgcc
     1081 cggtgccccc tacccttggc caggtgacac agtggttcac ggacgccttc cgcaccgagt
     1141 tcgagaaaca ggccgcacag atcggccggt tcaacctcgc cgtgttcggc aagaccggcg
     1201 tcggcaagtc gacgctgatc aacgccatct tcggcgagcc ggtggccgcc accggcatcg
     1261 gcgacccggt caccctcgac acccacctct acctcgacac ccgcggcacg ctgggcctgg
     1321 tcgacacccg cggcatcgag atcggtcgcg acgatgccga gctgatcaaa gaggtcagca
     1381 agtacgtgaa gcagatgcgc ggcaagccgg tcagtgagca gatccatgtg gcctggtact
     1441 gcgtgcgcgg catggaccgc cgcttcgaag aggtcgaggg cacgttcatc cgcacgctgc
     1501 acgacctggg cattccggtg ctcgtggtgt tcacccaggt gcccatgcac gacggccagt
     1561 accaccccga cgccgtcgaa ctggcccgct tggtcgaggc caagaacctg ccgatcgtcg
     1621 gcggacgtcc gttcatgacc tacgcgatga gggaccagtt caccggtcag ccgccctacg
     1681 gcttgatgga tgtgttgcag gccacctttc acgttgttcc tgacgcggtt cacgcagccc
     1741 tcgctgccgc ccagacgatc gatcttcagg cgaagcacca agccgcgcag cgacacatca
     1801 acgcgaccgt agccgcggcg gcgggtgcgg cagcggtgcc gatcccgttc tcgtcggcgg
     1861 ccctgctggt gccgatgcag ctgggcatga tgggtcgcat cgcgcacctc taccagttgc
     1921 cgttcgaccg ggcggcactg ctggcggtgg cctccacctc ggtggccacc accctgggcc
     1981 gcacggccgc cgccaacctg ctcaagttca ttcccggcgc cggctcgatc gccgggggtg
     2041 tgatcaacgc ctcggtcgcg tccggtttca ccctcgccat gggccagtcg tggctcaccg
     2101 tgtgccagcg ggccgcggcc ggcaagctgc ccacctcggt ggacggcgtg ctcgactcgg
     2161 cagcggtcaa gaagctcttc gaagacgagt tccgcaagcg catgccgacg atccgcaggc
     2221 aggacgagaa gtagccgcaa acatagagtg aatccgcccc cctcagacgg tgggtggatt
     2281 cactccatct tttgaccttc gccggcgacc cgtcaccgcc gagcctcgcg cgcccagcag
     2341 agtcacggtc cggccgcagc aatgtgggca tcgcacgcga actggtgatc tcgccgcgta
     2401 cggtcgatgc cccgcgctcg atcttcgccg aactcggcat cgccgcctcg tccgacgaga
     2461 acgcccgggt gctcgcggtg ctcagccgac tgtcagaccc ggcggccgag ctcgtgccgg
     2521 gtcgcggcgg ccctcgatag cggtcgattc cgcggggttg cgcgagccca tgccggtcgt
     2581 acagtgagcg aatggcgctc agcttcttcg atctgttcag catcgggatc ggtccgtcgt
     2641 cctcgcacac caccggtccg atgcgggccg cccggcgggt gctggacgat ctcgacgccg
     2701 ccggcgggct ggatcgggtc tggcgggtgc gggtggacct ctacggctcg ctgggctcga
     2761 ccggacgcgg acaccacagt gatcgggcag tgctgctcgg cctgctcggc catcgacccg
     2821 agacggtgga ccccgcagcg gtcggtcccg aagtcgaccg cattctggcc aggcatcgcc
     2881 tcagcctgct gaaccggcat ccgattcact tcaggccgaa tcgcgacctg atcctgcacc
     2941 aacgcaagtc gctgccccag catcccaacg gcatgcgggt gcgctgttac tcccgcgatc
     3001 ggaagccgat cgccgaccgg gtgctgttct cgatcggagg cggtttcgtg gtggacgccg
     3061 ccgacctggc cgaccc
//
LOCUS       DAOSUG010000387         3068 bp    DNA     linear   ENV 10-JUN-2024
DEFINITION  MAG TPA_asm: Micropruina sp. isolate AMR_MDS_2195
            NODE_12943_length_3068_cov_4.881513, whole genome shotgun sequence.
ACCESSION   DAOSUG010000387 DAOSUG010000000
VERSION     DAOSUG010000387.1
DBLINK      BioProject: PRJNA925477
            BioSample: SAMN32864262
            Sequence Read Archive: SRR4236673
KEYWORDS    WGS; ENV; Metagenome Assembled Genome; MAG; Third Party Data; TPA;
            TPA:assembly.
SOURCE      Micropruina sp. (wastewater metagenome)
  ORGANISM  Micropruina sp.
            Bacteria; Bacillati; Actinomycetota; Actinomycetes;
            Propionibacteriales; Nocardioidaceae; Micropruina.
REFERENCE   1  (bases 1 to 3068)
  AUTHORS   Abdulkadir,N., Saraiva,J.P., Zhang,J., Stolte,S., Gillor,O.,
            Harms,H. and Rocha,U.
  TITLE     Genome-centric analyses of 165 metagenomes show that mobile genetic
            elements are crucial for the transmission of antimicrobial
            resistance genes to pathogens in activated sludge and wastewater
  JOURNAL   Microbiol Spectr 12 (3), e0291823 (2024)
   PUBMED   38289113
REFERENCE   2  (bases 1 to 3068)
  AUTHORS   Abdulkadir,N.
  TITLE     Direct Submission
  JOURNAL   Submitted (24-MAR-2023) Environmental Microbiology, Helmholtz
            Center for Environmental Research -UFZ, Permoserstr 15, Leipzig
            04318, Germany
COMMENT     The annotation was added by the NCBI Prokaryotic Genome Annotation
            Pipeline (PGAP). Information about PGAP can be found here:
            https://www.ncbi.nlm.nih.gov/genome/annotation_prok/
            
            ##Genome-Assembly-Data-START##
            Assembly Method        :: SPAdes v. 3.11.1
            Genome Representation  :: Full
            Expected Final Version :: Yes
            Genome Coverage        :: Not Applicable
            Sequencing Technology  :: Illumina MiSeq
            ##Genome-Assembly-Data-END##
            
            ##Genome-Annotation-Data-START##
            Annotation Provider               :: NCBI
            Annotation Date                   :: 05/04/2023 04:57:10
            Annotation Pipeline               :: NCBI Prokaryotic Genome
                                                 Annotation Pipeline (PGAP)
            Annotation Method                 :: Best-placed reference protein
                                                 set; GeneMarkS-2+
            Annotation Software revision      :: 6.5
            Features Annotated                :: Gene; CDS; rRNA; tRNA; ncRNA
            Genes (total)                     :: 2,776
            CDSs (total)                      :: 2,750
            Genes (coding)                    :: 2,714
            CDSs (with protein)               :: 2,714
            Genes (RNA)                       :: 26
            tRNAs                             :: 24
            ncRNAs                            :: 2
            Pseudo Genes (total)              :: 36
            CDSs (without protein)            :: 36
            Pseudo Genes (ambiguous residues) :: 0 of 36
            Pseudo Genes (frameshifted)       :: 2 of 36
            Pseudo Genes (incomplete)         :: 32 of 36
            Pseudo Genes (internal stop)      :: 2 of 36
            ##Genome-Annotation-Data-END##
FEATURES             Location/Qualifiers
     source          1..3068
                     /organism="Micropruina sp."
                     /mol_type="genomic DNA"
                     /submitter_seqid="NODE_12943_length_3068_cov_4.881513"
                     /isolate="AMR_MDS_2195"
                     /isolation_source="Activated sludge of municipal
                     wastewater treatment plants"
                     /db_xref="taxon:2737536"
                     /environmental_sample
                     /geo_loc_name="Canada"
                     /lat_lon="49.25 N 123.1 W"
                     /collection_date="2015-06-16"
                     /metagenome_source="wastewater metagenome"
                     /note="metagenomic"
     gene            complement(<1..403)
                     /locus_tag="PLF56_11505"
     CDS             complement(<1..403)
                     /locus_tag="PLF56_11505"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_008363390.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="hypothetical protein"
                     /protein_id="HOQ54224.1"
                     /translation="MDTITEPATAVRGGRGFWGALWAGTRATVGALMGLAPHVMHHIG
                     FLAGAAFLTGFLGNSLLYVVGLLLSIPLLRRLRRRFGTRKAPAIGVAVFTALFALSAF
                     VIGPLFNPATPNQPDTPSPAASASASGTEDHE"
     gene            complement(412..1209)
                     /locus_tag="PLF56_11510"
     CDS             complement(412..1209)
                     /locus_tag="PLF56_11510"
                     /EC_number="2.5.1.145"
                     /inference="COORDINATES: protein motif:HMM:NF013915.2"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_component: GO:0016020 - membrane [Evidence IEA];
                     GO_function: GO:0008961 -
                     phosphatidylglycerol-prolipoprotein diacylglyceryl
                     transferase activity [Evidence IEA];
                     GO_process: GO:0009249 - protein lipoylation [Evidence
                     IEA];
                     GO_process: GO:0042158 - lipoprotein biosynthetic process
                     [Evidence IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="prolipoprotein diacylglyceryl transferase"
                     /protein_id="HOQ54225.1"
                     /translation="MWPEVFSIFGVGIQSYGLSKALALLVGAYLLGRAFTRIGYDKDL
                     AHSIALWATVWGFVGAKVYYLIANWDNLSWHLFGGSGFVWYGGLIAGAIAVIVMTRRH
                     DLPLGRVAGAMAAPLSVAYGIGRIGCFLAGDGTYGTPTDLPWGMAFPNGVVPVNVPVH
                     PVQLYEAAGAFLIAGILWALGRRLRPLAVFGSYLLLSGAARLLAEVVRINPHVLLGLT
                     EAQLIGIVSIILGVALILRDAFHPAVAPSDAPVGSESAVLSSHPVGR"
     gene            1584..2255
                     /locus_tag="PLF56_11515"
     CDS             1584..2255
                     /locus_tag="PLF56_11515"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_008363388.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_function: GO:0005524 - ATP binding [Evidence IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="ABC transporter ATP-binding protein"
                     /protein_id="HOQ54226.1"
                     /translation="MGSSGASSDGVRADVVLAGTAISKTYRQGLWPFVRRNHVLDGVS
                     LSLSRGEVVGLVGENGSGKSTLMQILVGGLAADAGAVELQGALGYCPQRPVVFERLTC
                     DEHFELFAVAYGMSRDERLRAQGTLYEELDFGRYAHERADRLSGGTLAKLNLALALLP
                     DPAVLLLDEPYAGFDWDTYLRFWELVARGRSFGRAVLVISHFIADEARFDRIIELRDG
                     KATPK"
     gene            2252..>3068
                     /locus_tag="PLF56_11520"
     CDS             2252..>3068
                     /locus_tag="PLF56_11520"
                     /inference="COORDINATES: protein motif:HMM:NF013245.2"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="ABC transporter permease"
                     /protein_id="HOQ54227.1"
                     /translation="MTAVGVLTRRFLLDYGRNPVNLVVLILIPVVFVWVAAEPMADAA
                     KLLGGTGRAVETATTGWAAAFLAALAMFFQVGQARSADRRLVLAGLPAPALVVARMST
                     GLLIAALASTAALATLFLRTAVDEPLRVAAGTMMFAVIYVGIGAAVGSVVTDQVNGTV
                     VILLVWILDVFFGPALTSPDRVQITRWMPTHFVTLWMVDLPLGHGGNLGDLAIALVWV
                     GGALLSSGLLLTRATRNARHPDGSHRPGWAGQVGKSFRMGMRQYRRNPALWVLL"
BASE COUNT          506 a         1019 c         1063 g          480 t
ORIGIN      
        1 cctcgtggtc ctcggtgccg gacgcgctgg cggaggccgc cggtgacggc gtgtctggtt
       61 ggttgggggt ggcggggttg aacagtggcc cgatcacgaa cgcggacagg gcgaacaggg
      121 cggtgaacac cgcgaccccg atcgcggggg ccttccgggt gccgaatcgg cgacgcagcc
      181 gtcgcagcag cgggatgctc agcagcaacc cgaccacata gaggagcgag ttgccgagga
      241 acccggtcag gaacgcggcc ccggccagga agccgatgtg atgcatcacg tgcggcgcca
      301 gtcccatcag ggcgccgacg gtggccctgg tcccggccca cagcgcaccc cagaacccgc
      361 gaccacccct cacggctgtg gccggctcgg tgatcgtgtc catggcgccg ctcacctccc
      421 gaccgggtga gagctaagga cggccgattc ggagccgaca ggagcatctg agggcgcaac
      481 tgcggggtgg aaggcgtctc gcaggatcag ggcgacgccc agaatgatac tgacgatgcc
      541 gatcagctgc gcctcggtga ggcccaacag gacgtgcggg ttgatccgga cgacctcggc
      601 gagcaggcgt gccgcgccgc tgagcagcag gtaggacccg aacacggcca gcggtcgcag
      661 cctgcgaccc agcgcccaca ggatgccggc gatcaggaag gcgccggcgg cctcatacag
      721 ctggacggga tgcaccggca cgttcaccgg caccaccccg ttggggaacg ccatccccca
      781 gggcaggtcg gtgggtgtgc cgtacgtgcc gtcaccggcg aggaagcagc cgatccggcc
      841 gatgccgtag gccaccgaga gcggggccgc catcgcgccc gccacccgtc caagggggag
      901 gtcgtggcgt cgggtcatca cgatcaccgc gatcgccccc gcgatcaggc cgccgtacca
      961 gacgaacccc gagccgccga acaggtgcca actgaggttg tcccagttcg cgatcaggta
     1021 gtacaccttg gcgccgacga atccccacac tgtcgcccac aacgcgatcg agtgcgccaa
     1081 gtccttgtcg tagccgatcc gcgtgaacgc tcgacccagc agatacgcgc cgaccagcag
     1141 cgccagcgcc ttactcagcc cataggactg gatccctaca ccaaatatcg agaacacctc
     1201 aggccacaca gcccactccc atcgtcaaag aagttccagg cgcacgacgt gaccgcgcgg
     1261 cctcagtgcc cagtctgaac cggtcacgtc aagcaacgag caggacccgg acaaggtttg
     1321 gtcaagatcc ccgtccccag cccggacgag gtgtcgcggc cgtaaggccc ggaaacttga
     1381 ccgaagcctg accccctgac ggtgatctcc gcactagagc gtgcctaccc tgatgacata
     1441 ccccttgggg gtgtcgtggc gccgccacgg gtcacccgac acaccacggc tcgggtcgtt
     1501 gaggaatggg cccttgtggc ggtctggcga agcccactgg cgggtgcgtg aaagtcacat
     1561 gggattcgaa ggcggtgatc gacatgggtt caagcggcgc ctcttctgat ggcgttcgag
     1621 cggacgtggt cctcgcgggg accgcgatct ccaagaccta tcgtcagggg ctgtggccgt
     1681 tcgtgcggcg caaccatgtg ctcgacggtg tgtcactgtc gctgagccgc ggcgaggtcg
     1741 tcgggctggt cggggagaac ggatcgggca aatcgacgct gatgcagatc ctggtcgggg
     1801 ggttggcagc cgacgccggc gccgtggagc tgcagggggc cttggggtac tgtccgcagc
     1861 gtccggtggt gttcgagcgc ctgacgtgcg atgaacattt cgagctattc gcggtggcct
     1921 acgggatgag ccgggatgag cggctccggg cgcagggaac cttgtacgag gaacttgact
     1981 ttgggcgata cgcgcacgag cgggcggacc ggctctccgg gggcaccctc gcgaaactga
     2041 atcttgccct ggctctgcta ccggacccgg cggtcctgct gctggacgag ccctatgccg
     2101 ggttcgattg ggacacctat cttcggttct gggagctcgt cgcgcgcggg cgctcattcg
     2161 ggcgggcagt gctggtgatc agtcatttca tcgccgacga ggcgcggttc gaccgcatca
     2221 tcgaactgcg tgacgggaag gcgaccccga aatgaccgcg gtcggggtgc tgacccgccg
     2281 attcctcctg gactacggcc gcaacccggt gaacctggtg gtcctgattc tgatcccggt
     2341 cgtgttcgtg tgggtggccg cggaaccgat ggccgatgcc gcgaaactgc tgggcggcac
     2401 tggccgggca gtcgagaccg cgaccaccgg ctgggccgcc gcgttcctgg cggcgctggc
     2461 gatgttcttc caggttggcc aggcgagatc ggcggaccgt cgtctggtcc tggcgggcct
     2521 ccccgcccca gccctcgttg tcgccagaat gtcgaccggc ctgctgatcg ctgcactggc
     2581 ctccacagcg gccctggcga cgctgttcct gcgcaccgca gtggacgagc cgctccgcgt
     2641 cgcggccggg acgatgatgt tcgccgtcat ctacgtcggg atcggcgccg cggtcggatc
     2701 ggtcgtcacg gaccaggtca acggcacggt ggtgatcctg ctcgtctgga tcctcgacgt
     2761 gttcttcgga ccggcgctca cctcgcccga ccgggtgcag atcacccgct ggatgcccac
     2821 ccacttcgtc acactgtgga tggtggacct gcccttgggc catgggggaa acctcggcga
     2881 cctcgccatc gcactggtgt gggtcggcgg agccctcctg tcgagcggac tgctcctgac
     2941 gcgcgcgaca cgcaacgctc ggcacccgga cggctcgcat cgaccgggct gggcggggca
     3001 agtcgggaag agcttccgga tggggatgcg acagtaccga cgcaacccag ccctctgggt
     3061 cctcctcg
//
LOCUS       DAOSUG010000388         3054 bp    DNA     linear   ENV 10-JUN-2024
DEFINITION  MAG TPA_asm: Micropruina sp. isolate AMR_MDS_2195
            NODE_13024_length_3054_cov_3.137713, whole genome shotgun sequence.
ACCESSION   DAOSUG010000388 DAOSUG010000000
VERSION     DAOSUG010000388.1
DBLINK      BioProject: PRJNA925477
            BioSample: SAMN32864262
            Sequence Read Archive: SRR4236673
KEYWORDS    WGS; ENV; Metagenome Assembled Genome; MAG; Third Party Data; TPA;
            TPA:assembly.
SOURCE      Micropruina sp. (wastewater metagenome)
  ORGANISM  Micropruina sp.
            Bacteria; Bacillati; Actinomycetota; Actinomycetes;
            Propionibacteriales; Nocardioidaceae; Micropruina.
REFERENCE   1  (bases 1 to 3054)
  AUTHORS   Abdulkadir,N., Saraiva,J.P., Zhang,J., Stolte,S., Gillor,O.,
            Harms,H. and Rocha,U.
  TITLE     Genome-centric analyses of 165 metagenomes show that mobile genetic
            elements are crucial for the transmission of antimicrobial
            resistance genes to pathogens in activated sludge and wastewater
  JOURNAL   Microbiol Spectr 12 (3), e0291823 (2024)
   PUBMED   38289113
REFERENCE   2  (bases 1 to 3054)
  AUTHORS   Abdulkadir,N.
  TITLE     Direct Submission
  JOURNAL   Submitted (24-MAR-2023) Environmental Microbiology, Helmholtz
            Center for Environmental Research -UFZ, Permoserstr 15, Leipzig
            04318, Germany
COMMENT     The annotation was added by the NCBI Prokaryotic Genome Annotation
            Pipeline (PGAP). Information about PGAP can be found here:
            https://www.ncbi.nlm.nih.gov/genome/annotation_prok/
            
            ##Genome-Assembly-Data-START##
            Assembly Method        :: SPAdes v. 3.11.1
            Genome Representation  :: Full
            Expected Final Version :: Yes
            Genome Coverage        :: Not Applicable
            Sequencing Technology  :: Illumina MiSeq
            ##Genome-Assembly-Data-END##
            
            ##Genome-Annotation-Data-START##
            Annotation Provider               :: NCBI
            Annotation Date                   :: 05/04/2023 04:57:10
            Annotation Pipeline               :: NCBI Prokaryotic Genome
                                                 Annotation Pipeline (PGAP)
            Annotation Method                 :: Best-placed reference protein
                                                 set; GeneMarkS-2+
            Annotation Software revision      :: 6.5
            Features Annotated                :: Gene; CDS; rRNA; tRNA; ncRNA
            Genes (total)                     :: 2,776
            CDSs (total)                      :: 2,750
            Genes (coding)                    :: 2,714
            CDSs (with protein)               :: 2,714
            Genes (RNA)                       :: 26
            tRNAs                             :: 24
            ncRNAs                            :: 2
            Pseudo Genes (total)              :: 36
            CDSs (without protein)            :: 36
            Pseudo Genes (ambiguous residues) :: 0 of 36
            Pseudo Genes (frameshifted)       :: 2 of 36
            Pseudo Genes (incomplete)         :: 32 of 36
            Pseudo Genes (internal stop)      :: 2 of 36
            ##Genome-Annotation-Data-END##
FEATURES             Location/Qualifiers
     source          1..3054
                     /organism="Micropruina sp."
                     /mol_type="genomic DNA"
                     /submitter_seqid="NODE_13024_length_3054_cov_3.137713"
                     /isolate="AMR_MDS_2195"
                     /isolation_source="Activated sludge of municipal
                     wastewater treatment plants"
                     /db_xref="taxon:2737536"
                     /environmental_sample
                     /geo_loc_name="Canada"
                     /lat_lon="49.25 N 123.1 W"
                     /collection_date="2015-06-16"
                     /metagenome_source="wastewater metagenome"
                     /note="metagenomic"
     gene            complement(<1..253)
                     /locus_tag="PLF56_11525"
     CDS             complement(<1..253)
                     /locus_tag="PLF56_11525"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_013161763.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="cytochrome O ubiquinol oxidase"
                     /protein_id="HOQ54228.1"
                     /translation="MTLLPALVPLLLPDWMDPEYLIHALGNWALWGVALVLIIECSIF
                     PILPGDSLLFTVGMFIAMDPPSITFAGMSQPMVLLVSCLV"
     gene            367..948
                     /locus_tag="PLF56_11530"
     CDS             367..948
                     /locus_tag="PLF56_11530"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_015069545.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_function: GO:0003723 - RNA binding [Evidence IEA];
                     GO_function: GO:0008173 - RNA methyltransferase activity
                     [Evidence IEA];
                     GO_process: GO:0006396 - RNA processing [Evidence IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="TrmH family RNA methyltransferase"
                     /protein_id="HOQ54229.1"
                     /translation="MGPHPEPWPDDPRLDPELLREGDRRNVIDRYRYWRLGAIVADLD
                     VHRHPLHVAIQNWEHDFNIGSIVRTANAFNVAAVHILGRRRWNRRGAMVTDRYLHVEH
                     HADLPSFFDSLARAGVVPVGVDNLPGSVPLETTELPQRCCLIFGSEGPGLTDEVVAGC
                     AALVSITQYGSTRSLNAGAAAAIAMYHWAHRWG"
     gene            1129..1653
                     /locus_tag="PLF56_11535"
     CDS             1129..1653
                     /locus_tag="PLF56_11535"
                     /inference="COORDINATES: ab initio
                     prediction:GeneMarkS-2+"
                     /note="Derived by automated computational analysis using
                     gene prediction method: GeneMarkS-2+."
                     /codon_start=1
                     /transl_table=11
                     /product="hypothetical protein"
                     /protein_id="HOQ54230.1"
                     /translation="MNSVGEYVVFYAPEAVQATVKRWRSMLVQRLISSAISVAIAAAV
                     WYFFAAQVGSWGPWLVGSALVVGLVWVVVSLTGLLIARKDAAQVQPGPAFAVTRDGVW
                     IAQLWLIWPEVGAVLANPGRFGRSATLAVHARDGRTASVPMEYLTALPASIDSAVRAL
                     SGGRCWIDLSRLDD"
     gene            1781..2803
                     /gene="fbaA"
                     /locus_tag="PLF56_11540"
     CDS             1781..2803
                     /gene="fbaA"
                     /locus_tag="PLF56_11540"
                     /EC_number="4.1.2.13"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_002520672.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_function: GO:0004332 - fructose-bisphosphate aldolase
                     activity [Evidence IEA];
                     GO_function: GO:0008270 - zinc ion binding [Evidence IEA];
                     GO_process: GO:0005975 - carbohydrate metabolic process
                     [Evidence IEA];
                     GO_process: GO:0006096 - glycolytic process [Evidence
                     IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="class II fructose-bisphosphate aldolase"
                     /protein_id="HOQ54231.1"
                     /translation="MPIATPEKYAEMLDAAKAGSFAFPAINVTSSQSLNAAIAGFAEA
                     GSDGIVQVSTGGAEYASGPTIKHMVTGAVALAEYAHVVAKNYSVNIVLHTDHCQKEKL
                     DTYVRPLIAISQERVDRGLEPLFQSHMWDGSAVPMEENLQIADQLLALTSKARQILEI
                     EVGVVGGEEDGVAAEINDKLYTTVEDGLRTIEVLGLGEKGRYITALTFGNVHGVYKPG
                     SVKLRPEVLKEIQDAVGAKYGREKPFDLVFHGGSGSTPQEISDAVDYGVIKMNVDTDT
                     QYAFTRPVAGWMLSNYDGVLKIDGEVGNKKMYDPRAWGKAAEAGMSARVIEACQNLRS
                     AGKSIG"
     gene            complement(2873..>3054)
                     /locus_tag="PLF56_11545"
     CDS             complement(2873..>3054)
                     /locus_tag="PLF56_11545"
                     /inference="COORDINATES: ab initio
                     prediction:GeneMarkS-2+"
                     /note="Derived by automated computational analysis using
                     gene prediction method: GeneMarkS-2+."
                     /codon_start=3
                     /transl_table=11
                     /product="hypothetical protein"
                     /protein_id="HOQ54232.1"
                     /translation="GALIEVYNFSEDLVELPAYVLRDRLGTTAVEQISGFDYSLEPDT
                     LRIRPYQPLWLTAKV"
BASE COUNT          523 a         1016 c         1039 g          476 t
ORIGIN      
        1 gcaccaggca ggagaccagc agcaccatcg gctggctcat gcccgcgaag gtgatcgagg
       61 gcgggtccat cgcgatgaac atgccgacgg tgaacagcag cgagtcgccg ggcaggatcg
      121 ggaagatcga gcactcgatg atcagcacca gggcgacgcc ccacagggcc cagttgccca
      181 gggcatggat gagatattcg gggtccatcc agtcgggcaa tagcagtggc acgagcgcag
      241 gaagcagggt cacagtggtt caggctaccg tggggcgctg tgcagccggt aagcgacagc
      301 tatgacaagg gccgacgtcc gccgtccgcc agcagcccgg ccgatccgac ggcgccgtcc
      361 gttgggctgg gcccccaccc cgaaccctgg ccggacgatc cgcgactcga ccccgaactg
      421 ctgcgcgagg gcgaccggcg caacgtgatc gaccgctacc gctactggcg tttgggcgcc
      481 atcgtcgccg acctcgacgt acaccggcac ccgctgcacg tggcgatcca gaactgggag
      541 cacgacttca acatcggctc gatcgtccgc accgccaatg ccttcaacgt ggcggccgtg
      601 cacattctcg gccggcgcag atggaaccgc cgcggcgcca tggtcaccga ccgttacctg
      661 cacgtggagc atcacgcgga cctcccctcg ttcttcgaca gcctggcgcg cgccggagtc
      721 gtcccggtcg gagtcgacaa cctgcccggc tcggtaccgc tcgaaaccac ggagttgccg
      781 caacgctgtt gcctgatctt cggctcggag ggccccgggc tcaccgacga ggtggtggca
      841 ggttgcgccg ctctggtctc gatcacgcag tacggatcca cccgttcgct gaacgccggc
      901 gcggcggccg cgatcgccat gtaccactgg gcgcaccgct gggggtgagg ccgggacgcg
      961 cgtccccacg gatgagcccg ctggaccgag gatgtaccag gctccgggtt gggcgcatct
     1021 ttcgagcaag gccgacccac tgaccggcgc gggcctcgga cggtccgccc gcccagaggg
     1081 tgcgagggcg ggggccgcgt cccggatgct ccccactacg atggcgccgt gaacagtgtc
     1141 ggcgagtacg tggtgttcta cgcacccgag gccgtgcagg ccacggtgaa acgctggcgc
     1201 tcaatgctgg ttcagcggtt gatctcgtcg gcgatctcgg tcgccatcgc cgcggccgtg
     1261 tggtatttct tcgcagcgca ggtgggttcg tggggtccct ggttggtcgg ttcggcgttg
     1321 gtggtcggcc tggtctgggt ggtggtcagc ctcaccgggc tgctgatcgc ccgcaaggac
     1381 gccgcgcagg tgcagccggg ccccgccttc gccgtgacgc gtgacggcgt gtggattgcc
     1441 cagttatggc tgatctggcc cgaggttggt gcggtgctcg ccaaccctgg ccgattcgga
     1501 cgctccgcca cgctcgccgt gcacgcccgc gacggacgaa ccgcgtccgt tccgatggag
     1561 tatctgacgg cgctgccggc cagcatcgac tcggccgtgc gggccctgtc gggcgggcgc
     1621 tgctggatcg acttgtcccg gttggacgac tgaacgcagc ggcgacgcac cgagcgtgaa
     1681 aaaggctcgc gcggccccgc gacttttgaa agactaggaa cgtaatcgca ggcgctgccg
     1741 acgatcggtg cgccacgcaa ccccgtgagg agcaaggtca atgcccatcg caacacccga
     1801 gaagtacgcc gagatgctgg acgccgccaa ggcgggttcg ttcgcctttc cggccatcaa
     1861 cgtcacctcg tcccagtccc tgaacgcggc catcgccggc ttcgccgagg ccggctccga
     1921 cggcatcgtc caggtcagca ccggtggcgc cgagtacgcc tcggggccga ccatcaagca
     1981 catggtgacc ggcgcggttg cccttgccga gtacgcccat gtcgtggcca agaactactc
     2041 ggtcaacatc gtgctgcaca ccgaccactg ccagaaggaa aagctcgaca cctacgtccg
     2101 gccgctgatc gcgatctcgc aggagcgggt cgaccgtggc ctggaaccgc tgttccagtc
     2161 gcacatgtgg gacggctccg cagttccgat ggaagagaac ctgcagatcg ccgaccagtt
     2221 gctcgcgctg actagcaagg ctcgccagat tctcgagatc gaggtcggcg tggtcggcgg
     2281 cgaagaggac ggcgtggccg ccgagatcaa cgacaagctg tacaccacgg tcgaagacgg
     2341 cctgcgcacc atcgaggtgc ttggcctcgg tgaaaagggc cggtacatca ccgcgctgac
     2401 cttcggcaac gtgcacggcg tctacaagcc gggttcggtg aagctgcgtc ccgaggtgct
     2461 caaggagatc caggacgcgg tcggcgccaa gtacggccgc gagaagccgt tcgacctggt
     2521 gttccatggc ggctcgggat cgaccccgca agagatcagc gacgccgtcg actacggcgt
     2581 gatcaagatg aacgtcgaca ccgacaccca gtacgcgttc acccggccgg tggccggctg
     2641 gatgctgagc aactacgacg gcgtgctcaa gatcgacggc gaggtcggca acaagaagat
     2701 gtacgacccg cgggcctggg gcaaggccgc cgaggccggc atgtccgcgc gggtcatcga
     2761 ggcctgccag aacctgcgct cggccggcaa gagcatcggc tgatcgctcg tccgacacgc
     2821 gatgcccggg ccaggtgccc gggcatcgct gcgtttcagc agcgcacagg attcacactt
     2881 tcgcggtcag ccacagcggc tggtatgggc gaatgcgcag ggtgtcgggt tcgagggagt
     2941 aatcgaatcc gctgatctgt tcgacagccg tcgtgccgag ccggtcgcgc agcacgtacg
     3001 ccggcagctc gaccaggtct tcgctgaagt tgtacacctc gatcagcgcg ccct
//
LOCUS       DAOSUG010000389         3049 bp    DNA     linear   ENV 10-JUN-2024
DEFINITION  MAG TPA_asm: Micropruina sp. isolate AMR_MDS_2195
            NODE_13043_length_3049_cov_4.749833, whole genome shotgun sequence.
ACCESSION   DAOSUG010000389 DAOSUG010000000
VERSION     DAOSUG010000389.1
DBLINK      BioProject: PRJNA925477
            BioSample: SAMN32864262
            Sequence Read Archive: SRR4236673
KEYWORDS    WGS; ENV; Metagenome Assembled Genome; MAG; Third Party Data; TPA;
            TPA:assembly.
SOURCE      Micropruina sp. (wastewater metagenome)
  ORGANISM  Micropruina sp.
            Bacteria; Bacillati; Actinomycetota; Actinomycetes;
            Propionibacteriales; Nocardioidaceae; Micropruina.
REFERENCE   1  (bases 1 to 3049)
  AUTHORS   Abdulkadir,N., Saraiva,J.P., Zhang,J., Stolte,S., Gillor,O.,
            Harms,H. and Rocha,U.
  TITLE     Genome-centric analyses of 165 metagenomes show that mobile genetic
            elements are crucial for the transmission of antimicrobial
            resistance genes to pathogens in activated sludge and wastewater
  JOURNAL   Microbiol Spectr 12 (3), e0291823 (2024)
   PUBMED   38289113
REFERENCE   2  (bases 1 to 3049)
  AUTHORS   Abdulkadir,N.
  TITLE     Direct Submission
  JOURNAL   Submitted (24-MAR-2023) Environmental Microbiology, Helmholtz
            Center for Environmental Research -UFZ, Permoserstr 15, Leipzig
            04318, Germany
COMMENT     The annotation was added by the NCBI Prokaryotic Genome Annotation
            Pipeline (PGAP). Information about PGAP can be found here:
            https://www.ncbi.nlm.nih.gov/genome/annotation_prok/
            
            ##Genome-Assembly-Data-START##
            Assembly Method        :: SPAdes v. 3.11.1
            Genome Representation  :: Full
            Expected Final Version :: Yes
            Genome Coverage        :: Not Applicable
            Sequencing Technology  :: Illumina MiSeq
            ##Genome-Assembly-Data-END##
            
            ##Genome-Annotation-Data-START##
            Annotation Provider               :: NCBI
            Annotation Date                   :: 05/04/2023 04:57:10
            Annotation Pipeline               :: NCBI Prokaryotic Genome
                                                 Annotation Pipeline (PGAP)
            Annotation Method                 :: Best-placed reference protein
                                                 set; GeneMarkS-2+
            Annotation Software revision      :: 6.5
            Features Annotated                :: Gene; CDS; rRNA; tRNA; ncRNA
            Genes (total)                     :: 2,776
            CDSs (total)                      :: 2,750
            Genes (coding)                    :: 2,714
            CDSs (with protein)               :: 2,714
            Genes (RNA)                       :: 26
            tRNAs                             :: 24
            ncRNAs                            :: 2
            Pseudo Genes (total)              :: 36
            CDSs (without protein)            :: 36
            Pseudo Genes (ambiguous residues) :: 0 of 36
            Pseudo Genes (frameshifted)       :: 2 of 36
            Pseudo Genes (incomplete)         :: 32 of 36
            Pseudo Genes (internal stop)      :: 2 of 36
            ##Genome-Annotation-Data-END##
FEATURES             Location/Qualifiers
     source          1..3049
                     /organism="Micropruina sp."
                     /mol_type="genomic DNA"
                     /submitter_seqid="NODE_13043_length_3049_cov_4.749833"
                     /isolate="AMR_MDS_2195"
                     /isolation_source="Activated sludge of municipal
                     wastewater treatment plants"
                     /db_xref="taxon:2737536"
                     /environmental_sample
                     /geo_loc_name="Canada"
                     /lat_lon="49.25 N 123.1 W"
                     /collection_date="2015-06-16"
                     /metagenome_source="wastewater metagenome"
                     /note="metagenomic"
     gene            <1..1457
                     /locus_tag="PLF56_11550"
     CDS             <1..1457
                     /locus_tag="PLF56_11550"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_008357785.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=3
                     /transl_table=11
                     /product="circularly permuted type 2 ATP-grasp protein"
                     /protein_id="HOQ54233.1"
                     /translation="EAGVAQRVRALEAFLADVYSYGKVFDDGVIPRRVVVSSPHFHRV
                     MHGVVPPNGVRVHVSGIDLIRDNEGTFRVLEDNVRVPSGVSYVMTNRRAMFSALPEVT
                     SRHRIRPVEQYPLQLLAALRAAAPHGISDPTVVVLTPGVYNSAYFEHALLARMMGVEL
                     VEGRDLVCRGGRVSIRTTQGLRPVHVIYRRLDDEFLDPAAFRADSMLGCAGLINAVRA
                     GNVTLANCVGNGVADDKLVYTYVPNLIRYYLNEEPVIPGVDTWRMEDPDSRAEVLDRL
                     DELVLKPVDGSGGKGIVIGPAASQRKLDKLRHDVLANPRGWIAQPVVQLSTVPTMIEG
                     KMAPRHVDLRPFAVNSGTGIYVLPGGLTRVALPEGELIVNSSQGGGSKDTWVLARDGM
                     RAQRKRARPQVLPMGVPLADIGDEGVQQQQEQQQQTRVVSPVERVETSVGVSPVERVE
                     TPSGAIGVPPVEPSEAPSDAFSANRRHDRLEASC"
     gene            1451..2383
                     /locus_tag="PLF56_11555"
     CDS             1451..2383
                     /locus_tag="PLF56_11555"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_008357786.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="alpha-E domain-containing protein"
                     /protein_id="HOQ54234.1"
                     /translation="MLSRIAESLFWIGRYLERAEDTCRIVEVHLQLLLDDPTVDQEAA
                     GQSLLEVMGSTPPEGRPADPQYVLTALCYDDESPASVATALGGARESARRARETVSTE
                     MWESINTTWNSVRAGRLQRMRPATAFKHIRERCAVISGTADSTMSHDEGWHFLILGRS
                     IERADMTARLIQSASITSGSQSAWNNVLRGCGAHHAFVRTYGGVTTDRDAAEFLLLDR
                     LFPRSLVHTLTAAEQALAELGQTERRAGIEDDAQRLLGTARAALEYRPPGEVLFDLPS
                     RMADLQWVCNQASTAISQRYFEGATAAVWRGGEQ"
     gene            2380..>3049
                     /locus_tag="PLF56_11560"
     CDS             2380..>3049
                     /locus_tag="PLF56_11560"
                     /inference="COORDINATES: protein motif:HMM:NF013961.2"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="transglutaminase family protein"
                     /protein_id="HOQ54235.1"
                     /translation="MTRQIRIVHHTGYAYAGTVVGSHNEVRMTPRASREQWVMNARID
                     IAPTPWVLSYTDYWGTRVTAFEIREKHARLNVTSTATVEVTRPPVVAPGIGWDELRDD
                     SFTDGMVEMLSVSEHVDPGDELCGYAASVAREVSPAETVFEVIQRIRERVRYVPGVTH
                     VQTRARDVWESGAGVCQDMVHVAIGALRTVGIPARYVSGYLMPSSNPVPGEPRTAASH
                     AWLQY"
BASE COUNT          469 a         1008 c         1098 g          474 t
ORIGIN      
        1 tcgaggccgg cgttgcgcaa cgggtccggg ccctcgaggc cttcctggcc gacgtgtaca
       61 gctacggcaa ggtgttcgac gacggcgtga ttccgcgccg cgtggtggtc agctcgccgc
      121 acttccaccg ggtgatgcac ggggtggtac cgcccaacgg ggtgcgggtg cacgtgtcgg
      181 gcatcgacct gattcgcgac aacgagggca ccttccgggt gctcgaagac aacgtgcggg
      241 tgccgtccgg ggtgtcgtac gtgatgacca accggcgggc gatgttctcg gcgctgcccg
      301 aggtgaccag ccggcaccgc atcaggccgg tcgagcaata cccgctgcaa ctgctggcgg
      361 ccctgcgcgc cgctgccccg cacggcatca gcgacccgac cgtggtggtg ctcaccccgg
      421 gcgtctacaa ctcggcctac ttcgagcatg cgctgctggc ccgcatgatg ggcgtcgagc
      481 tggtcgaggg ccgcgacctg gtgtgccgtg gcgggcgggt ctcgatccgc accacgcagg
      541 gcctgcggcc ggtgcacgtg atctaccgcc gcctcgatga cgagtttctc gacccggcgg
      601 cgttccgggc cgattcgatg ctgggctgcg ccggtctgat caatgcggtg cgggccggca
      661 acgtgacgct ggcgaactgc gtcggcaacg gggtggccga cgacaagctc gtctacacct
      721 acgtgccgaa cctgatccgg tactacctca acgaagaacc cgtcattcct ggcgtcgata
      781 cgtggcgcat ggaggatccc gactcacgag cagaggtgct ggaccggctg gacgagttgg
      841 tgctgaagcc ggtggacggt tccggcggca agggcatcgt gatcggtccg gcggccagcc
      901 agcgcaagct cgacaagctg cggcacgacg tgctggccaa cccgcgcggg tggatcgcgc
      961 agcccgtggt gcaactctcg accgtgccga ccatgatcga aggcaagatg gccccccggc
     1021 acgtcgacct gcggcccttc gcggtcaaca gcggcaccgg catctacgtg ctgcccggcg
     1081 ggctgacccg ggtggcgctg cccgagggtg agctgatcgt caacagctcc cagggcggtg
     1141 gctcgaagga cacctgggtg ctggccaggg acggcatgcg cgcgcagcgc aaacgagcaa
     1201 ggccccaggt gctcccgatg ggcgtgcccc tggccgacat cggcgatgag ggcgtgcagc
     1261 agcagcagga gcaacagcag cagacgcggg tcgtttcgcc ggtcgaacgt gtcgagactt
     1321 cggttggtgt gtcgccggtc gagcgtgtcg agaccccctc cggcgcgatc ggtgttccgc
     1381 cggtcgagcc ttccgaggcc ccgtccgatg cgttctcggc caaccgtcgc cacgaccggc
     1441 tggaggcgtc atgctgagcc ggatcgccga atcgctgttc tggatcggcc gttacctgga
     1501 gcgcgccgaa gacacctgcc ggatcgtcga ggtacacctg cagttactgc tggacgaccc
     1561 caccgtcgat caggaggcgg ccggccagtc gctgctggag gtgatgggct ccacgccgcc
     1621 cgagggtcgt ccggccgacc cgcagtacgt gctgaccgcg ctgtgctacg acgacgaatc
     1681 gcccgcctcg gtggcgaccg cgctgggcgg cgcccgcgaa tcggcccgcc gggcccgcga
     1741 aaccgtctcg accgagatgt gggagtcgat caacaccacc tggaactcgg tgcgggccgg
     1801 acggctgcag cgcatgcgtc cggcgaccgc gttcaagcac atccgcgaac ggtgtgcggt
     1861 catctcgggc acggccgact cgacgatgag ccatgacgag ggctggcact tcctgattct
     1921 cggacgctcc atcgagcggg ccgacatgac ggcacggctg atccagtcgg cgtccatcac
     1981 gtcggggtcg cagagtgcgt ggaacaacgt gttgcgcggg tgtggggcgc accacgcatt
     2041 cgtccgcacc tacggtggcg tcaccactga ccgcgacgct gccgagttcc tgctgctcga
     2101 ccggctgttt ccacggtcgt tggtgcacac cctgaccgcc gccgagcagg cgctggccga
     2161 gttgggccag accgaacgcc gcgccggcat cgaggacgac gcgcaacgcc tgctgggtac
     2221 ggcgcgggcg gcgctggagt accgtccacc gggcgaggtg ctgttcgacc tgccgagccg
     2281 gatggccgac ctgcagtggg tctgcaacca ggccagcacg gcgatcagcc agcgctattt
     2341 cgagggagcg acggccgcgg tgtggcgagg aggcgagcag tgaccaggca gattcggatc
     2401 gtgcaccaca cgggctacgc ctacgccggc accgtggtgg gctcgcacaa cgaggtgcgc
     2461 atgaccccgc gggccagccg tgaacagtgg gtgatgaacg cccggatcga cattgcgccg
     2521 accccctggg tgctcagcta caccgactac tggggcaccc gggtcacggc gttcgagatc
     2581 cgcgagaagc acgctcggct caacgtgacc tcgacggcga cggtcgaggt gacccgtccg
     2641 ccggtggtgg cgccgggtat cggctgggac gagttgcgcg acgactcgtt caccgacgga
     2701 atggtcgaga tgctcagcgt gagcgagcac gtcgatccgg gcgacgagtt gtgtgggtac
     2761 gcggcgtccg tcgcgcgtga ggtgagtccg gccgagacgg tcttcgaagt gatccagcgc
     2821 attcgcgagc gggtgcggta cgtgcccggg gtcacccatg tgcagacccg cgcccgcgac
     2881 gtgtgggaat ccggtgcggg cgtctgtcag gacatggtgc acgtggcgat cggcgcattg
     2941 cgcacggtcg gtattccggc ccgctacgtg tcgggctacc tgatgccgtc gtcgaatccg
     3001 gtgccgggtg agccgcgcac cgccgcctcg cacgcctggc tgcagtact
//
LOCUS       DAOSUG010000390         3036 bp    DNA     linear   ENV 10-JUN-2024
DEFINITION  MAG TPA_asm: Micropruina sp. isolate AMR_MDS_2195
            NODE_13118_length_3036_cov_3.748742, whole genome shotgun sequence.
ACCESSION   DAOSUG010000390 DAOSUG010000000
VERSION     DAOSUG010000390.1
DBLINK      BioProject: PRJNA925477
            BioSample: SAMN32864262
            Sequence Read Archive: SRR4236673
KEYWORDS    WGS; ENV; Metagenome Assembled Genome; MAG; Third Party Data; TPA;
            TPA:assembly.
SOURCE      Micropruina sp. (wastewater metagenome)
  ORGANISM  Micropruina sp.
            Bacteria; Bacillati; Actinomycetota; Actinomycetes;
            Propionibacteriales; Nocardioidaceae; Micropruina.
REFERENCE   1  (bases 1 to 3036)
  AUTHORS   Abdulkadir,N., Saraiva,J.P., Zhang,J., Stolte,S., Gillor,O.,
            Harms,H. and Rocha,U.
  TITLE     Genome-centric analyses of 165 metagenomes show that mobile genetic
            elements are crucial for the transmission of antimicrobial
            resistance genes to pathogens in activated sludge and wastewater
  JOURNAL   Microbiol Spectr 12 (3), e0291823 (2024)
   PUBMED   38289113
REFERENCE   2  (bases 1 to 3036)
  AUTHORS   Abdulkadir,N.
  TITLE     Direct Submission
  JOURNAL   Submitted (24-MAR-2023) Environmental Microbiology, Helmholtz
            Center for Environmental Research -UFZ, Permoserstr 15, Leipzig
            04318, Germany
COMMENT     The annotation was added by the NCBI Prokaryotic Genome Annotation
            Pipeline (PGAP). Information about PGAP can be found here:
            https://www.ncbi.nlm.nih.gov/genome/annotation_prok/
            
            ##Genome-Assembly-Data-START##
            Assembly Method        :: SPAdes v. 3.11.1
            Genome Representation  :: Full
            Expected Final Version :: Yes
            Genome Coverage        :: Not Applicable
            Sequencing Technology  :: Illumina MiSeq
            ##Genome-Assembly-Data-END##
            
            ##Genome-Annotation-Data-START##
            Annotation Provider               :: NCBI
            Annotation Date                   :: 05/04/2023 04:57:10
            Annotation Pipeline               :: NCBI Prokaryotic Genome
                                                 Annotation Pipeline (PGAP)
            Annotation Method                 :: Best-placed reference protein
                                                 set; GeneMarkS-2+
            Annotation Software revision      :: 6.5
            Features Annotated                :: Gene; CDS; rRNA; tRNA; ncRNA
            Genes (total)                     :: 2,776
            CDSs (total)                      :: 2,750
            Genes (coding)                    :: 2,714
            CDSs (with protein)               :: 2,714
            Genes (RNA)                       :: 26
            tRNAs                             :: 24
            ncRNAs                            :: 2
            Pseudo Genes (total)              :: 36
            CDSs (without protein)            :: 36
            Pseudo Genes (ambiguous residues) :: 0 of 36
            Pseudo Genes (frameshifted)       :: 2 of 36
            Pseudo Genes (incomplete)         :: 32 of 36
            Pseudo Genes (internal stop)      :: 2 of 36
            ##Genome-Annotation-Data-END##
FEATURES             Location/Qualifiers
     source          1..3036
                     /organism="Micropruina sp."
                     /mol_type="genomic DNA"
                     /submitter_seqid="NODE_13118_length_3036_cov_3.748742"
                     /isolate="AMR_MDS_2195"
                     /isolation_source="Activated sludge of municipal
                     wastewater treatment plants"
                     /db_xref="taxon:2737536"
                     /environmental_sample
                     /geo_loc_name="Canada"
                     /lat_lon="49.25 N 123.1 W"
                     /collection_date="2015-06-16"
                     /metagenome_source="wastewater metagenome"
                     /note="metagenomic"
     gene            <1..361
                     /locus_tag="PLF56_11565"
     CDS             <1..361
                     /locus_tag="PLF56_11565"
                     /inference="COORDINATES: ab initio
                     prediction:GeneMarkS-2+"
                     /note="Derived by automated computational analysis using
                     gene prediction method: GeneMarkS-2+."
                     /codon_start=2
                     /transl_table=11
                     /product="hypothetical protein"
                     /protein_id="HOQ54236.1"
                     /translation="RSDGPAGAGENVRGGQGLWDEVFAPQNLAVALGRVERNRGAAGV
                     DGVSTQQLRDWCREHWTATRAALDAGTYRPSPVRQVMIPKPDGGQRKLGVPTVLDRLI
                     QQAIAQVNRPRFDAAAV"
     gene            381..659
                     /locus_tag="PLF56_11570"
                     /pseudo
     CDS             381..659
                     /locus_tag="PLF56_11570"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_076611738.1"
                     /note="incomplete; partial in the middle of a contig;
                     missing C-terminus; Derived by automated computational
                     analysis using gene prediction method: Protein Homology.
                     GO_function: GO:0003677 - DNA binding [Evidence IEA];
                     GO_function: GO:0004803 - transposase activity [Evidence
                     IEA];
                     GO_process: GO:0006313 - transposition, DNA-mediated
                     [Evidence IEA]"
                     /pseudo
                     /codon_start=1
                     /transl_table=11
                     /product="transposase"
     gene            1319..2782
                     /gene="ltrA"
                     /locus_tag="PLF56_11575"
     CDS             1319..2782
                     /gene="ltrA"
                     /locus_tag="PLF56_11575"
                     /EC_number="2.7.7.49"
                     /inference="COORDINATES: protein motif:HMM:TIGR04416.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_function: GO:0003964 - RNA-directed DNA polymerase
                     activity [Evidence IEA];
                     GO_process: GO:0000373 - Group II intron splicing
                     [Evidence IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="group II intron reverse transcriptase/maturase"
                     /protein_id="HOQ54237.1"
                     /translation="MSPVNTDELELALMLARQRVLKIQTKLHRWARDDLDRRFDDLFN
                     LVADPAFVLVAWDRVSGNKGAATAGVDRRTASSITAGQGIEVFLDELRSQLKDRSFRP
                     LPVRERMIPKTGGALRRLGIPTVADRVVQASLKLVLEPIFEADFLPCSYGFRPKRRAH
                     DAVAEVRYLATRPRCYDWVVEGDIKACFDEIDHTALMGRVRRRVGDNRVLGLVKAFLK
                     AGIFTEDGLLADSTAGTPQGGILSPLLANVALSVLDEHIAGLPGGPATGSVERARRLR
                     HGQPNFRLVRYADDWCLMVRGTRAHAEALQEDIAAVLATMGLRLAPDKTLITHIDEGL
                     DFLGWRIQRRRKRGTSCSYVYVHPSTKAVLAVKRKIKTLRRTVELNQPLDDLLRRINA
                     TLQGWAGYFRAGVSSATFSYLSHYTWQTVWRWLRRKHRKSTWKDLRRQYCGGGWWPAT
                     EDRVLIDLEKIGTTRYRYRGAVIPSPWPTDEEDTTAA"
     gene            2896..3036
                     /locus_tag="PLF56_11580"
                     /pseudo
     CDS             2896..3036
                     /locus_tag="PLF56_11580"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_076611738.1"
                     /note="incomplete; partial in the middle of a contig;
                     missing N-terminus and C-terminus; Derived by automated
                     computational analysis using gene prediction method:
                     Protein Homology."
                     /pseudo
                     /codon_start=1
                     /transl_table=11
                     /product="IS3 family transposase"
BASE COUNT          548 a          865 c         1090 g          533 t
ORIGIN      
        1 gcgtagtgat ggtccggcgg gtgctgggga gaacgtcagg ggcggccagg ggttgtggga
       61 tgaggtgttc gccccgcaga acctggctgt tgcgttggga cgtgtcgaac gcaatcgggg
      121 cgcggccggt gtggatggtg tgagcacgca gcaactgcgt gactggtgcc gtgaacattg
      181 gacggcgacg cgggcggcgt tggatgcggg tacgtaccgt ccgtcgccgg tgcgtcaggt
      241 gatgattccc aaacctgatg gcgggcaacg gaagttgggg gttccgacgg tgctggatcg
      301 gctgattcag caggccatcg cgcaagtgaa ccgccccagg tttgatgccg ctgctgtttg
      361 agtccggaag gatgagcagc atgcctaaga agatcgatcc tgcgttgcgt gaccgtgcgg
      421 tgcggttggt gaccgaacat cagcaggagt attcgtcgtt gaccgcggcc tccgaggcgg
      481 tcgcgcgcca actcggcgtg ggcaaggaat cggtgcgtcg ctgggtggtc caagcccaga
      541 ttgatggcgg acaacgcccg ggtgtaacga gcgaagaaat cgacgaaatc aaacgactga
      601 aagctgaaaa ccgaaggctg cgtgaggatg tcgcgattct gaaagcggca acgactttct
      661 tcgcggggga actgtgcgcc acgaggcgct gggtatatcg agaggcggtg agagaccgtc
      721 tccttcgggt cgtcgcagcg gcctgaggaa gcatcggggc agccgatcgc cgggaacgac
      781 ggtgatcggt ggcaagcggt cccgagaagg caagccgtgc cggcactgcc agacggtgcg
      841 ggcttgtcga gcaaggtcag aaggtgcagc gcaagcgaac cagtgtcaga cgctccgtaa
      901 ccgcgaagcc ggctccaacc tggtggatat gggccggtat gcagtgcacg aacccgagcg
      961 ggtggggttt gactccgaag ccgtttagtt ccagcggtgg ggaggccacg gtgaaggcct
     1021 gcggcgtaac catggcgatg ctgccggagt agagctgggc gcctccctga ccaatcgatg
     1081 acctggtgaa cgtgggaacc accctgcggg accgctcatt tcgtttccgg cagccagtcg
     1141 gggcgggtgt gacgactcgg gttcggagtc aaggaaccgc atttcagggt ggggcggagg
     1201 cctcgtagta gtcgcgggcg gtgacgtgcc cgtcgtggag accgagaaag gcggtcacag
     1261 ggcgaagggg gccagcaaga catgcagtgc ggaaactgga aggtcaggag aggaagttat
     1321 gtcgccggtg aataccgacg agctggagtt ggcgctgatg ctggcgcggc aacgggtact
     1381 gaagatccag accaagctgc accgttgggc acgcgatgat cttgatcgcc ggttcgacga
     1441 tctgttcaac ctcgtcgccg atcccgcgtt cgtgttggtg gcgtgggatc gggtcagcgg
     1501 gaataagggt gccgccacgg ccggggtgga tcggcgcacc gcgtcgtcca tcactgccgg
     1561 gcagggcatc gaggtgttcc tcgatgaact gcggtcgcag ttgaaggacc gcagtttccg
     1621 cccgttgccg gtgcgcgagc ggatgatccc caagaccggt ggtgcgttgc gccgcttggg
     1681 gattcccacc gtggccgatc gggtggtcca ggcatcgttg aagttggtgt tggagccgat
     1741 ctttgaggcg gatttcctgc cgtgttccta cggtttccgt ccgaagcgcc gcgctcatga
     1801 tgcggtggcc gaagtgcgtt acctcgcaac acgtccccgg tgttacgact gggttgttga
     1861 gggagacatc aaggcctgtt tcgatgagat cgaccacacg gccctgatgg gccgggtgcg
     1921 tcgacgcgtc ggggacaacc gtgtcctggg attggtgaag gcgttcctca aggcgggcat
     1981 cttcaccgag gacggcctgc tggcggacag caccgccgga accccgcagg gtgggattct
     2041 ttcgccgctg ttggccaatg tggcgttgtc ggtgctcgat gaacacattg ccgggttgcc
     2101 gggtggtccg gctaccggct cggttgagcg cgctcgacga cttcgtcacg ggcagccgaa
     2161 ctttcggctg gtccgctacg ccgacgactg gtgtctgatg gtgcggggca ccagagccca
     2221 cgccgaagca ctacaggagg acatcgccgc cgtgttggcg acgatggggt tgcgtctggc
     2281 accggacaag accctgatca cccatatcga cgaggggctg gacttcctcg ggtggcgcat
     2341 ccagcgtcgc cgcaagcggg gcaccagctg cagctacgtc tatgttcatc cgtccacgaa
     2401 ggccgtgttg gcggtcaaac ggaagatcaa gacgctgcgc cgaacagttg aactcaacca
     2461 gccgctcgat gacctgctgc gccgcatcaa cgcgacgctg cagggatggg cgggctattt
     2521 ccgggccggg gtgtcctcgg cgaccttctc ctacctcagt cactacacgt ggcagacggt
     2581 gtggcgctgg ctccgacgaa aacaccgcaa gtcgacctgg aaagacctgc gccgccaata
     2641 ttgcggcggc ggctggtggc cagccaccga ggaccgtgtg ctgatcgatc tggagaagat
     2701 cggcacgacc cgctaccgct accgcggtgc ggtcattccc tcgccctggc cgaccgacga
     2761 ggaggacacc acagcagcct gaccgggtct tgtggagagc ccggtgcatt gaaaggtgca
     2821 cgccgggttc gggaagcggc ccgggaaaac ggaacagccc caacggttgt caccgcgtcc
     2881 cgggccgact ttcaccgacc cccgcaaccg gtgatcgccg cgttcatcga cgatcaacgc
     2941 gccgccgggc acgcggtcga gtcgatctgc cgggtcctgc acgagcaggg ctgccagatc
     3001 gccgcgcgca cctaccgggc ccaacgacaa ggcatc
//
LOCUS       DAOSUG010000391         3036 bp    DNA     linear   ENV 10-JUN-2024
DEFINITION  MAG TPA_asm: Micropruina sp. isolate AMR_MDS_2195
            NODE_13119_length_3036_cov_3.513586, whole genome shotgun sequence.
ACCESSION   DAOSUG010000391 DAOSUG010000000
VERSION     DAOSUG010000391.1
DBLINK      BioProject: PRJNA925477
            BioSample: SAMN32864262
            Sequence Read Archive: SRR4236673
KEYWORDS    WGS; ENV; Metagenome Assembled Genome; MAG; Third Party Data; TPA;
            TPA:assembly.
SOURCE      Micropruina sp. (wastewater metagenome)
  ORGANISM  Micropruina sp.
            Bacteria; Bacillati; Actinomycetota; Actinomycetes;
            Propionibacteriales; Nocardioidaceae; Micropruina.
REFERENCE   1  (bases 1 to 3036)
  AUTHORS   Abdulkadir,N., Saraiva,J.P., Zhang,J., Stolte,S., Gillor,O.,
            Harms,H. and Rocha,U.
  TITLE     Genome-centric analyses of 165 metagenomes show that mobile genetic
            elements are crucial for the transmission of antimicrobial
            resistance genes to pathogens in activated sludge and wastewater
  JOURNAL   Microbiol Spectr 12 (3), e0291823 (2024)
   PUBMED   38289113
REFERENCE   2  (bases 1 to 3036)
  AUTHORS   Abdulkadir,N.
  TITLE     Direct Submission
  JOURNAL   Submitted (24-MAR-2023) Environmental Microbiology, Helmholtz
            Center for Environmental Research -UFZ, Permoserstr 15, Leipzig
            04318, Germany
COMMENT     The annotation was added by the NCBI Prokaryotic Genome Annotation
            Pipeline (PGAP). Information about PGAP can be found here:
            https://www.ncbi.nlm.nih.gov/genome/annotation_prok/
            
            ##Genome-Assembly-Data-START##
            Assembly Method        :: SPAdes v. 3.11.1
            Genome Representation  :: Full
            Expected Final Version :: Yes
            Genome Coverage        :: Not Applicable
            Sequencing Technology  :: Illumina MiSeq
            ##Genome-Assembly-Data-END##
            
            ##Genome-Annotation-Data-START##
            Annotation Provider               :: NCBI
            Annotation Date                   :: 05/04/2023 04:57:10
            Annotation Pipeline               :: NCBI Prokaryotic Genome
                                                 Annotation Pipeline (PGAP)
            Annotation Method                 :: Best-placed reference protein
                                                 set; GeneMarkS-2+
            Annotation Software revision      :: 6.5
            Features Annotated                :: Gene; CDS; rRNA; tRNA; ncRNA
            Genes (total)                     :: 2,776
            CDSs (total)                      :: 2,750
            Genes (coding)                    :: 2,714
            CDSs (with protein)               :: 2,714
            Genes (RNA)                       :: 26
            tRNAs                             :: 24
            ncRNAs                            :: 2
            Pseudo Genes (total)              :: 36
            CDSs (without protein)            :: 36
            Pseudo Genes (ambiguous residues) :: 0 of 36
            Pseudo Genes (frameshifted)       :: 2 of 36
            Pseudo Genes (incomplete)         :: 32 of 36
            Pseudo Genes (internal stop)      :: 2 of 36
            ##Genome-Annotation-Data-END##
FEATURES             Location/Qualifiers
     source          1..3036
                     /organism="Micropruina sp."
                     /mol_type="genomic DNA"
                     /submitter_seqid="NODE_13119_length_3036_cov_3.513586"
                     /isolate="AMR_MDS_2195"
                     /isolation_source="Activated sludge of municipal
                     wastewater treatment plants"
                     /db_xref="taxon:2737536"
                     /environmental_sample
                     /geo_loc_name="Canada"
                     /lat_lon="49.25 N 123.1 W"
                     /collection_date="2015-06-16"
                     /metagenome_source="wastewater metagenome"
                     /note="metagenomic"
     gene            57..503
                     /locus_tag="PLF56_11585"
     CDS             57..503
                     /locus_tag="PLF56_11585"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_014847116.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="type II toxin-antitoxin system VapC family
                     toxin"
                     /protein_id="HOQ54238.1"
                     /translation="MIVDANVLLYAVDDQSHFHTTARTWLDEAMNGVERVGLPWVSLM
                     SFQRIITHPRVTSTPLTPAVAWSFITDWLDADQAWVPTPGGRHRDILGRLLTEGDLRG
                     NLVTDAHLAALAIEHGTSICSFDSDFARFPGLHWITPRALVVAAGS"
     gene            complement(609..1289)
                     /locus_tag="PLF56_11590"
     CDS             complement(609..1289)
                     /locus_tag="PLF56_11590"
                     /inference="COORDINATES: protein motif:HMM:NF014906.2"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_function: GO:0003677 - DNA binding [Evidence IEA];
                     GO_process: GO:0006355 - regulation of DNA-templated
                     transcription [Evidence IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="TetR/AcrR family transcriptional regulator"
                     /protein_id="HOQ54239.1"
                     /translation="MTGTPARARERLTRDRVLTAAVDLADTHGLAAVSMRRLAAELGV
                     VPMALYKHVADKDELVDGMLDRALAEIEPTATDEGWRASVRGRVLAARVVQLRHPWLR
                     RVLETRTSRTPTVLRYTDALIGLFRAGGFSDPLTHHVMHALGSRMWGFTQELFDDPDV
                     PAEPIDPAAAAQLAQAFPNVFAVAMAARHDDGSVVSYGCDDQFEFEFALDLLLDGFVR
                     LHEQGWQP"
     gene            1386..2096
                     /locus_tag="PLF56_11595"
     CDS             1386..2096
                     /locus_tag="PLF56_11595"
                     /inference="COORDINATES: protein motif:HMM:NF025687.2"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="DUF4386 domain-containing protein"
                     /protein_id="HOQ54240.1"
                     /translation="MTVDTRAARTAGLLYLLTFVASIAALPLLSPVLQDPRFVLTQQP
                     SAGVVAGCLLDLVNAAACVGTAVVLYPLLRGRSPSAALGFVTSRVLEAAIIATGVICL
                     LAVVTLQHSDSDDIARVALAEGLVAVRNWTFLLGPGLMPAVNALLLGTALLHSALVPR
                     GIPLLGLIGAPLQLISVIGTLFGLNDQVSVWSAIAVLPIFMWELALGLWLTFRGPAEH
                     TRWPRSARDASVSPLTKR"
     gene            2339..2476
                     /locus_tag="PLF56_11600"
     CDS             2339..2476
                     /locus_tag="PLF56_11600"
                     /inference="COORDINATES: ab initio
                     prediction:GeneMarkS-2+"
                     /note="Derived by automated computational analysis using
                     gene prediction method: GeneMarkS-2+."
                     /codon_start=1
                     /transl_table=11
                     /product="hypothetical protein"
                     /protein_id="HOQ54241.1"
                     /translation="MSHSSARTQPPRQRPYSVVGLVALGTAGACHRAETVRLAQANGR
                     L"
     gene            2572..2763
                     /locus_tag="PLF56_11605"
     CDS             2572..2763
                     /locus_tag="PLF56_11605"
                     /inference="COORDINATES: ab initio
                     prediction:GeneMarkS-2+"
                     /note="Derived by automated computational analysis using
                     gene prediction method: GeneMarkS-2+."
                     /codon_start=1
                     /transl_table=11
                     /product="hypothetical protein"
                     /protein_id="HOQ54242.1"
                     /translation="MPIRAMRGAARSLVGLLSLPARPVPRGRRVRGVAVDLIGAAVAG
                     AAAWWIGTTVAPAVPAMQC"
     gene            2757..>3036
                     /locus_tag="PLF56_11610"
     CDS             2757..>3036
                     /locus_tag="PLF56_11610"
                     /inference="COORDINATES: ab initio
                     prediction:GeneMarkS-2+"
                     /note="Derived by automated computational analysis using
                     gene prediction method: GeneMarkS-2+."
                     /codon_start=1
                     /transl_table=11
                     /product="hypothetical protein"
                     /protein_id="HOQ54243.1"
                     /translation="MLTAFGVIIAGCLLVSAALRLRRRSFPPPETRLIAGRPAIGLHA
                     WAAEHWLEIVVDCALALTCLGVLIADAARGGRWLPWTLLLLVPIVWFGG"
BASE COUNT          421 a         1081 c         1002 g          532 t
ORIGIN      
        1 accgacgtcg ccggcaccct tgagttgctc gatcagtacg acgcggagga tgccgggtga
       61 tcgtcgacgc caacgtcctg ctctacgccg tcgatgacca gtcccatttc cacaccaccg
      121 cccgaacctg gctggacgag gcgatgaacg gcgttgaacg cgtggggctg ccgtgggtat
      181 cgctgatgtc gttccagcgc atcatcaccc acccccgcgt gacctcgacc ccgctgaccc
      241 cagcggttgc gtggagcttc atcaccgact ggctcgacgc cgaccaggcc tgggtaccca
      301 caccaggagg ccgccaccgc gacatcctcg gacgcctcct caccgaaggc gatctgcgcg
      361 ggaacctggt caccgacgcc cacctcgctg ctcttgccat cgagcacggc accagcatct
      421 gctccttcga cagcgacttc gcccgatttc ctggcctcca ctggatcacc ccccgggcgt
      481 tggtagtagc ggctggctct tgaatcttga cttcagagtg tccttgagcg cggtggcggc
      541 tttctgcttg cggtagaggg ccccggttcc ccgtgtgcgt cggctggtga gttgcgctat
      601 agcgcttgct agggctgcca gccctgctcg tgcagccgga cgaagccgtc gagcagcagg
      661 tccagggcga actcgaactc gaactgatcg tcgcagccgt agctcaccac cgacccgtcg
      721 tcgtgcctgg ctgccatcgc caccgcgaac acgttcggaa acgcctgcgc cagttgcgcc
      781 gcggcggccg gatcgatcgg ctcggcgggc acgtccgggt catcgaacaa ctcctgggtg
      841 aaaccccaca tgcgactgcc gagggcatgc atcacgtgat gggtgagcgg gtcggagaac
      901 cccccggccc ggaacagtcc gatcagcgca tccgtgtacc ggagcacggt gggggtgcgg
      961 ctggtgcggg tttcgagcac gcggcgcaac caggggtgcc gcagctgtac cacccgagcc
     1021 gccagcaccc tgccccgcac ggacgcccgc cagccctcgt ccgttgccgt gggctcgatc
     1081 tcggcaagcg cccggtcgag catgccgtcg acgagttcat ccttgtccgc gacgtgtttg
     1141 tagagggcca tcggcaccac gcccagctcg gccgcgagcc ggcgcatgct gaccgcggcc
     1201 agcccgtggg tgtcggcgag gtcgaccgcc gccgtcaaca cgcgatcacg ggtcagccgc
     1261 tcccgagccc tagcaggggt gccggtcatc gcgcctccgc ttcctcggct tgacgagcgt
     1321 acggtgtaca ccttagcgtt gcggcatgag tgtacggtgt acacctttga gcgataggga
     1381 cccacgtgac cgtcgacacc cgagccgcac gaaccgccgg cctgctgtac ctgctcacct
     1441 tcgtcgcgtc catcgccgcc ctgccgctgc tgtcgcccgt gcttcaggat ccgcgtttcg
     1501 tgctgaccca gcagcccagc gccggcgtgg tggcgggctg cctgctcgat ctcgtcaacg
     1561 ccgccgcttg cgtgggcacc gcggtggtcc tctacccact gttgcggggt cgcagcccgt
     1621 ccgccgcgct gggtttcgtc acgtcacggg tgctggaggc agcgatcatc gcgaccgggg
     1681 tgatctgcct gctggccgtg gtcacgctgc agcactccga ttcggacgac atcgcccggg
     1741 tcgcgctcgc cgagggcctg gtggccgtgc gcaactggac gtttctgctc ggccccgggc
     1801 tgatgccggc cgtcaatgcg ttgctgctcg gcacggcgct gctgcatagt gctctggtgc
     1861 cgcggggaat cccgctgctg gggctgatcg gcgcgccgct tcagctgatc tcggtgatcg
     1921 gcacgctgtt cgggctcaac gatcaggtgt cggtctggtc ggccatcgcg gtgctgccga
     1981 tctttatgtg ggaactcgct ctcggcctgt ggttgacctt ccgcgggccg gccgagcaca
     2041 cacgctggcc ccggtcggca cgtgacgcgt ccgtctctcc actgacgaag cggtgatcga
     2101 cgcacgatcc tctgctgagc ttgatgaaac ccctactagc aagcctttcg acgaggcatg
     2161 gtgcctcaac aagttcgacc tgcgatgaag ctccgtgcga cctcacggcc ccgacatcac
     2221 tcgtgatgtg tccctcgatc ccgggtcgta tcgcgaccga ggagaacgcc gcgaggtgcg
     2281 cgctcaggcg cagccaatcg gtaagtgatg tggaggacac gccactaggt tgtacgccat
     2341 gtcacactcc agcgcacgga cgcagccgcc gcgacagcgt ccgtacagcg tggtcgggtt
     2401 ggtcgccctc ggcactgcgg gtgcatgcca ccgagccgag accgtccggc tcgcccaagc
     2461 gaacggccgg ctgtgacgaa acccggccgc ggcacccggt tcagcgacga cgcacccgtc
     2521 acgcacaccg ttgggacgcg cggagtcggg gccccggttc gaaccgcagg cgtgcccatc
     2581 cgggccatgc gcggcgccgc ccgctccctg gtcggcctgc tcagcctgcc cgcgcggccg
     2641 gtcccacgcg gacgccgcgt ccgtggcgtg gccgtcgacc tgatcggtgc cgccgtggcc
     2701 ggcgcggccg cgtggtggat cggaacgacg gtggcgccgg ccgttccggc catgcagtgc
     2761 tgaccgcgtt cggggtgatc atcgccggat gcctgctggt ctccgccgct ctgcgactgc
     2821 gacggcgcag ctttccgcca ccagaaaccc ggttgatcgc cggacggccc gcgatcggct
     2881 tgcacgcctg ggccgctgag cactggctcg agatcgtggt cgattgcgcg ctggcactga
     2941 cctgtcttgg cgtgttgatc gccgacgcgg ctcgcggcgg ccgctggctg ccctggacgc
     3001 tgctgttgct cgtgccgatc gtctggttcg gcggcc
//
LOCUS       DAOSUG010000392         3035 bp    DNA     linear   ENV 10-JUN-2024
DEFINITION  MAG TPA_asm: Micropruina sp. isolate AMR_MDS_2195
            NODE_13124_length_3035_cov_3.777517, whole genome shotgun sequence.
ACCESSION   DAOSUG010000392 DAOSUG010000000
VERSION     DAOSUG010000392.1
DBLINK      BioProject: PRJNA925477
            BioSample: SAMN32864262
            Sequence Read Archive: SRR4236673
KEYWORDS    WGS; ENV; Metagenome Assembled Genome; MAG; Third Party Data; TPA;
            TPA:assembly.
SOURCE      Micropruina sp. (wastewater metagenome)
  ORGANISM  Micropruina sp.
            Bacteria; Bacillati; Actinomycetota; Actinomycetes;
            Propionibacteriales; Nocardioidaceae; Micropruina.
REFERENCE   1  (bases 1 to 3035)
  AUTHORS   Abdulkadir,N., Saraiva,J.P., Zhang,J., Stolte,S., Gillor,O.,
            Harms,H. and Rocha,U.
  TITLE     Genome-centric analyses of 165 metagenomes show that mobile genetic
            elements are crucial for the transmission of antimicrobial
            resistance genes to pathogens in activated sludge and wastewater
  JOURNAL   Microbiol Spectr 12 (3), e0291823 (2024)
   PUBMED   38289113
REFERENCE   2  (bases 1 to 3035)
  AUTHORS   Abdulkadir,N.
  TITLE     Direct Submission
  JOURNAL   Submitted (24-MAR-2023) Environmental Microbiology, Helmholtz
            Center for Environmental Research -UFZ, Permoserstr 15, Leipzig
            04318, Germany
COMMENT     The annotation was added by the NCBI Prokaryotic Genome Annotation
            Pipeline (PGAP). Information about PGAP can be found here:
            https://www.ncbi.nlm.nih.gov/genome/annotation_prok/
            
            ##Genome-Assembly-Data-START##
            Assembly Method        :: SPAdes v. 3.11.1
            Genome Representation  :: Full
            Expected Final Version :: Yes
            Genome Coverage        :: Not Applicable
            Sequencing Technology  :: Illumina MiSeq
            ##Genome-Assembly-Data-END##
            
            ##Genome-Annotation-Data-START##
            Annotation Provider               :: NCBI
            Annotation Date                   :: 05/04/2023 04:57:10
            Annotation Pipeline               :: NCBI Prokaryotic Genome
                                                 Annotation Pipeline (PGAP)
            Annotation Method                 :: Best-placed reference protein
                                                 set; GeneMarkS-2+
            Annotation Software revision      :: 6.5
            Features Annotated                :: Gene; CDS; rRNA; tRNA; ncRNA
            Genes (total)                     :: 2,776
            CDSs (total)                      :: 2,750
            Genes (coding)                    :: 2,714
            CDSs (with protein)               :: 2,714
            Genes (RNA)                       :: 26
            tRNAs                             :: 24
            ncRNAs                            :: 2
            Pseudo Genes (total)              :: 36
            CDSs (without protein)            :: 36
            Pseudo Genes (ambiguous residues) :: 0 of 36
            Pseudo Genes (frameshifted)       :: 2 of 36
            Pseudo Genes (incomplete)         :: 32 of 36
            Pseudo Genes (internal stop)      :: 2 of 36
            ##Genome-Annotation-Data-END##
FEATURES             Location/Qualifiers
     source          1..3035
                     /organism="Micropruina sp."
                     /mol_type="genomic DNA"
                     /submitter_seqid="NODE_13124_length_3035_cov_3.777517"
                     /isolate="AMR_MDS_2195"
                     /isolation_source="Activated sludge of municipal
                     wastewater treatment plants"
                     /db_xref="taxon:2737536"
                     /environmental_sample
                     /geo_loc_name="Canada"
                     /lat_lon="49.25 N 123.1 W"
                     /collection_date="2015-06-16"
                     /metagenome_source="wastewater metagenome"
                     /note="metagenomic"
     gene            complement(<1..418)
                     /locus_tag="PLF56_11615"
     CDS             complement(<1..418)
                     /locus_tag="PLF56_11615"
                     /inference="COORDINATES: ab initio
                     prediction:GeneMarkS-2+"
                     /note="Derived by automated computational analysis using
                     gene prediction method: GeneMarkS-2+."
                     /codon_start=1
                     /transl_table=11
                     /product="two pore domain potassium channel family
                     protein"
                     /protein_id="HOQ54244.1"
                     /translation="MTDAWRWVERNPSAVLLYVQLGGILLYPYLEGPGDQLGTLVLSL
                     FGMLVLGLAVATVRATPSLTWVAVLIGVPAVALTFVDVFTGNEQPWHLLSDCFHAAFY
                     AYTLVALLRYMFADDKVTTDELYAIGATFTIGIWLFA"
     gene            641..2512
                     /locus_tag="PLF56_11620"
     CDS             641..2512
                     /locus_tag="PLF56_11620"
                     /inference="COORDINATES: ab initio
                     prediction:GeneMarkS-2+"
                     /note="Derived by automated computational analysis using
                     gene prediction method: GeneMarkS-2+."
                     /codon_start=1
                     /transl_table=11
                     /product="hypothetical protein"
                     /protein_id="HOQ54245.1"
                     /translation="MQPPQSRRGLRTGIASLLAIMLACAGVAAAPGPAHAASASFCAD
                     GTQPMATAAEVEAFPAGTTVTGSTVVQGTTPSGFTGSYLGHVEGFFGKGKDLLLFQLS
                     SPTIDGTGVLKAAGIWAGMSGSPVYAADGSLIGAVSYSLNADNLPVAGVTPAEYMKGI
                     GSTLAAPAGVKLTATNLKKASGLRPNSAQADALSSGSALHPVGLANVAAASTQGTALT
                     NSLLARVPKNSSTAAERLRSRSFAAVGAAAVGSAANDPLVVGGNIAVLYSTGDAAIGG
                     VGTVTAICGTDVWAFGHYMDLAGKTTYALANASAAMVVPDGTGTAGSYKQVTAIGDAQ
                     GTITQDRRAGVKGTLGAVTGYPVSLNVSSAAGVVVDSRGSTVVLPEAGPDVAASLVGN
                     AVIEDLDNYYRGTLRFTWTISYRTGTGATGTIKKTQVYAGTDVVADGPAYDVGDDVYA
                     LIQNDFDDVAITGVAMDLTLVSEDAIVYKPSGVEYKKGTTWTSLSGKSLKPNASYSVR
                     PVYRQSVNGKLSGPSVAGATRTMSLSKKAIKKGSLSYVAGSTDNNCGEDGDECELDDV
                     DADSFSDLIAQLKVVNRNDAMAQLVSYSVKGSTSKSRATFTGPGVVDGSVKVSFKIG"
     gene            complement(2553..>3035)
                     /locus_tag="PLF56_11625"
     CDS             complement(2553..>3035)
                     /locus_tag="PLF56_11625"
                     /inference="COORDINATES: protein motif:HMM:NF018533.2"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="hypothetical protein"
                     /protein_id="HOQ54246.1"
                     /translation="LGPDWNPAVAAGNIAAAGHLDDATIGGALLDQRNLAGVGTLYAS
                     ESLFLERLNPWTPPRDLTPQQVAALIERAHRLLDHNKNHAVQSTTGSRRHGETNYVHA
                     RSGQPCRRCGGPVRVAPIGTPPRERTMFYCPACQGGLGPTDDGRPQQRLGSSGSRYRG
                     "
BASE COUNT          512 a         1022 c         1069 g          432 t
ORIGIN      
        1 aggcgaacag ccagatgccg atggtgaacg tggcgccgat ggcgtagagc tcgtcggtgg
       61 tcaccttgtc gtcggcgaac atgtagcgca gcagcgccac cagcgtgtag gcgtagaagg
      121 cggcgtgaaa gcagtccgac agcaggtgcc agggttgttc gttaccggtg aacacgtcca
      181 cgaaggtgag cgcaacggca ggcacgccga tcaacaccgc cacccaggtc agcgacggcg
      241 tggcccgcac cgtggcgacg gccaggccca gcaccagcat gccgaacagg gacagcacca
      301 gggtgccgag ctggtcgccg gggccttcga ggtaggggta cagcagaatg ccgccgagtt
      361 gcacgtagag cagcacagcg ctcgggttgc gctcgaccca tcgccacgcg tccgtcatgg
      421 cgggcagcct aacccgggat cgaggccggc ccggtcagcc ggatccgggc cacacggggc
      481 tgagcgggca aagggctggc accgctacac ccaccgactg aatagttgct gaatcgttgc
      541 ccgctcggct tgtgctgcac cgatcaccgg gcatcgtgtg cacagcagcc ttgccgatca
      601 gcatgctgcg gtccacccct cggagagaag gtcgactcac atgcaaccac cacagtcgcg
      661 tcgaggtctg cgcacgggca tagcgagcct gctggccatc atgctggcct gcgccggtgt
      721 ggccgccgct cccggcccgg cccacgcagc gtccgcctcg ttctgtgccg acggcaccca
      781 gccgatggcc accgccgccg aggtcgaggc cttcccggca ggcaccaccg tgaccggcag
      841 cacggtcgtg cagggcacga cgccgtccgg gttcacgggc agctacctcg gccatgtcga
      901 gggcttcttc ggcaagggca aggacctgct gctgttccag ctcagcagcc ccaccatcga
      961 cggcacgggc gttctcaagg cggccggaat ctgggccggc atgtcgggtt cgccggttta
     1021 tgccgccgac ggctcgctga tcggggcggt gtcgtacagc ctcaacgccg acaatctgcc
     1081 ggtcgcgggc gtcacccccg ccgagtacat gaagggcatc ggcagcacgc tggcggcacc
     1141 ggccggcgtc aaactgaccg cgaccaacct caagaaggcc agtgggctgc gtccgaactc
     1201 ggctcaggcg gacgccctga gcagcggctc cgcgctgcat ccggtggggc tcgccaacgt
     1261 ggcggcggcc tcgacgcagg gcaccgcgct gaccaactcg ctgctggcgc gggtgccgaa
     1321 gaacagcagc acggccgccg agcggctgcg cagtcggtcg ttcgccgcgg tcggcgcggc
     1381 cgcggtcggc agcgcggcca atgatccgct ggtggtcggc ggcaacatcg cggtgctgta
     1441 cagcaccggc gacgcggcca tcggcggcgt gggcacggtc accgcgatct gcggcaccga
     1501 cgtgtgggcg ttcgggcact acatggacct cgccggcaag accacctacg ccctggccaa
     1561 tgccagtgcc gccatggtgg ttccggacgg caccggcacg gccggctcgt acaagcaggt
     1621 gaccgcgatc ggcgacgccc aaggcacgat cacccaagac cgccgcgccg gcgtgaaggg
     1681 caccctcggt gccgtcaccg gctacccggt gagcctcaac gtgagcagcg ctgccggcgt
     1741 ggtcgtcgac tcacgcggct ccaccgtggt gctgcccgag gcagggcccg acgtggccgc
     1801 gtccctggtg ggcaacgccg tgatcgaaga cctcgacaac tactaccgcg gcacattgcg
     1861 gttcacctgg accatctcgt accgcaccgg gaccggggcc acgggcacca tcaagaagac
     1921 gcaggtctac gccggcaccg acgtggtcgc ggacggcccc gcctacgacg tcggcgacga
     1981 cgtctatgcc ctgattcaaa acgacttcga cgacgtcgcg atcaccggcg tcgccatgga
     2041 cctgacgctg gtcagcgaag acgcgatcgt ctacaagccc agcggcgtgg agtacaaaaa
     2101 gggcaccacg tggacctcgc tgtcgggcaa gtcgctcaag ccgaacgcgt cgtactcggt
     2161 tcggccggtg tatcggcagt cggtcaacgg caaactgagc ggtccgagcg tcgccggggc
     2221 gacccgcacg atgtcgctgt cgaagaaggc gatcaagaag ggctcgctga gctatgtggc
     2281 cggttcgacc gataacaact gcggcgagga cggcgacgag tgcgagttgg acgacgtgga
     2341 cgccgacagc ttcagtgatc tgatcgcgca actcaaggtc gtgaaccgca acgacgccat
     2401 ggcgcaactg gtcagctact cggtcaaggg cagcaccagc aagtcacggg ccacgttcac
     2461 cggccccggt gtggtggacg gttcggtgaa ggtgagcttc aagatcggct gatcgcgtca
     2521 cgtggcgccg gcgcagtcgg cgcgacccgg cgctaaccgc gatatctcga accggacgac
     2581 cccagccgct gctggggtcg tccgtcgtct gtcgggccca gacccccttg gcaggcgggg
     2641 cagtagaaca tggtgcgctc acgcgggggc gtcccgatcg gggcgacccg cacgggtccg
     2701 ccgcagcgcc ggcacggttg gccgctgcgg gcatgcacgt agttggtctc gccgtgccgg
     2761 cggctgccgg tggtggactg cacagcgtgg ttcttgttgt gatcgagcaa ccggtgggca
     2821 cgctcgatca gggccgcgac ctgctggggg gtgaggtcgc ggggcggcgt ccaggggttg
     2881 agcctttcga gaaacagcga ttccgaggca tacagcgtgc cgacgccggc caggtttcgc
     2941 tggtcgagca gcgcgccgcc gatggtggcg tcgtcgaggt gcccggcggc ggcgatgttg
     3001 cccgcggcca ccgccgggtt ccagtcgggg ccgag
//
LOCUS       DAOSUG010000393         3030 bp    DNA     linear   ENV 10-JUN-2024
DEFINITION  MAG TPA_asm: Micropruina sp. isolate AMR_MDS_2195
            NODE_13150_length_3030_cov_4.906891, whole genome shotgun sequence.
ACCESSION   DAOSUG010000393 DAOSUG010000000
VERSION     DAOSUG010000393.1
DBLINK      BioProject: PRJNA925477
            BioSample: SAMN32864262
            Sequence Read Archive: SRR4236673
KEYWORDS    WGS; ENV; Metagenome Assembled Genome; MAG; Third Party Data; TPA;
            TPA:assembly.
SOURCE      Micropruina sp. (wastewater metagenome)
  ORGANISM  Micropruina sp.
            Bacteria; Bacillati; Actinomycetota; Actinomycetes;
            Propionibacteriales; Nocardioidaceae; Micropruina.
REFERENCE   1  (bases 1 to 3030)
  AUTHORS   Abdulkadir,N., Saraiva,J.P., Zhang,J., Stolte,S., Gillor,O.,
            Harms,H. and Rocha,U.
  TITLE     Genome-centric analyses of 165 metagenomes show that mobile genetic
            elements are crucial for the transmission of antimicrobial
            resistance genes to pathogens in activated sludge and wastewater
  JOURNAL   Microbiol Spectr 12 (3), e0291823 (2024)
   PUBMED   38289113
REFERENCE   2  (bases 1 to 3030)
  AUTHORS   Abdulkadir,N.
  TITLE     Direct Submission
  JOURNAL   Submitted (24-MAR-2023) Environmental Microbiology, Helmholtz
            Center for Environmental Research -UFZ, Permoserstr 15, Leipzig
            04318, Germany
COMMENT     The annotation was added by the NCBI Prokaryotic Genome Annotation
            Pipeline (PGAP). Information about PGAP can be found here:
            https://www.ncbi.nlm.nih.gov/genome/annotation_prok/
            
            ##Genome-Assembly-Data-START##
            Assembly Method        :: SPAdes v. 3.11.1
            Genome Representation  :: Full
            Expected Final Version :: Yes
            Genome Coverage        :: Not Applicable
            Sequencing Technology  :: Illumina MiSeq
            ##Genome-Assembly-Data-END##
            
            ##Genome-Annotation-Data-START##
            Annotation Provider               :: NCBI
            Annotation Date                   :: 05/04/2023 04:57:10
            Annotation Pipeline               :: NCBI Prokaryotic Genome
                                                 Annotation Pipeline (PGAP)
            Annotation Method                 :: Best-placed reference protein
                                                 set; GeneMarkS-2+
            Annotation Software revision      :: 6.5
            Features Annotated                :: Gene; CDS; rRNA; tRNA; ncRNA
            Genes (total)                     :: 2,776
            CDSs (total)                      :: 2,750
            Genes (coding)                    :: 2,714
            CDSs (with protein)               :: 2,714
            Genes (RNA)                       :: 26
            tRNAs                             :: 24
            ncRNAs                            :: 2
            Pseudo Genes (total)              :: 36
            CDSs (without protein)            :: 36
            Pseudo Genes (ambiguous residues) :: 0 of 36
            Pseudo Genes (frameshifted)       :: 2 of 36
            Pseudo Genes (incomplete)         :: 32 of 36
            Pseudo Genes (internal stop)      :: 2 of 36
            ##Genome-Annotation-Data-END##
FEATURES             Location/Qualifiers
     source          1..3030
                     /organism="Micropruina sp."
                     /mol_type="genomic DNA"
                     /submitter_seqid="NODE_13150_length_3030_cov_4.906891"
                     /isolate="AMR_MDS_2195"
                     /isolation_source="Activated sludge of municipal
                     wastewater treatment plants"
                     /db_xref="taxon:2737536"
                     /environmental_sample
                     /geo_loc_name="Canada"
                     /lat_lon="49.25 N 123.1 W"
                     /collection_date="2015-06-16"
                     /metagenome_source="wastewater metagenome"
                     /note="metagenomic"
     gene            <1..1184
                     /locus_tag="PLF56_11630"
     CDS             <1..1184
                     /locus_tag="PLF56_11630"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_013601308.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=3
                     /transl_table=11
                     /product="PrgI family protein"
                     /protein_id="HOQ54247.1"
                     /translation="GGQLLYRRRVVKPRPVGTLALPGDQARLREYTDPETGAGMIHDP
                     TAGTLTVVCEVTHPAFVLLDPGEQERRVTSWGRVLATVCRSGRIATLQVLERTLPDSG
                     TGLAEWWEAHGTADDSWAAQTYAELIDRAGPAGERHATTLSLALDMNASARQIRTAGG
                     GLRGAAAVLRQEMNTLVAALRSADLLPSGWLSPGQIAVILRSAYDPAIAATLERHGQL
                     GQSLATAGPVAVNESWARLRTDSAHHAVLWVSEWPRSLVYPGFLSPVLLSTGIQRSFS
                     LLCTPMRSDQAARDIRKKKVEHISDQAQRAKIGQIEDAAQTAEYHDVLQQEADLTAGH
                     GVLRYTGLIAVSAPTVEELDAGVAAIEQAAIQASCETRLLVGQQAAAFTAAALPLCRR
                     I"
     gene            1272..2474
                     /locus_tag="PLF56_11635"
     CDS             1272..2474
                     /locus_tag="PLF56_11635"
                     /inference="COORDINATES: ab initio
                     prediction:GeneMarkS-2+"
                     /note="Derived by automated computational analysis using
                     gene prediction method: GeneMarkS-2+."
                     /codon_start=1
                     /transl_table=11
                     /product="hypothetical protein"
                     /protein_id="HOQ54248.1"
                     /translation="MPTFHDPLADAAEASEAIRGLAHASRVFDGPADTYTVFGDLTAA
                     MRSLRQVLDQLAATHLAHRERAHHDDGDHLAGDRSALAAADELHQAGTLLDQAHDHLN
                     AAFSHSGRIAWHPAPAQAGLDHDPATSTATAGTLRLTVWPDAPVGEHQRYAYRITDTT
                     TGQELEGRDLFTGAGQPVGPDHAIHDLATFLGAAGEARQYALDHPDTEPEHAGLFPSW
                     VAEAARTNADALTELTEPAERTAIAEASRPERRWINVVFLQGEDADEVLDLIDRDGTA
                     AGVEHLKGWDYGEETTQAALENGYVYDEPPTGALDRVITGEDGYTLTYNPFAGHVSLL
                     REYAPASDVLEDAVVPAREAITGIGAPPAPQPAQASATRRTALGADKDWFAGPARTTG
                     SAGRGLSL"
     gene            2471..>3030
                     /locus_tag="PLF56_11640"
     CDS             2471..>3030
                     /locus_tag="PLF56_11640"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_015582351.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="ATP-binding protein"
                     /protein_id="HOQ54249.1"
                     /translation="MTGTEPERLHTSVLVAPAAERRRLRKQRRQAAARLQAEQRAVEK
                     GAARAKAKAEKAERRATTYLPAAGEPGPAALKSPGRFRLPRHQDTSATLAGAYPFVAE
                     GGLGSDGVFVGQDLYSGGSFVYDPWVLYARGIITAPNIVLAGIVGSGKSSLAKSLYTR
                     SLPFGRRVYVPGDPKGEHTAVAEAVGG"
BASE COUNT          474 a         1204 c          990 g          362 t
ORIGIN      
        1 ccggcggcca gctcctctac cgacgccgag tcgtcaagcc ccgcccggtc ggcacgctcg
       61 cgctgcccgg cgaccaggcg cgcctgcggg agtacaccga cccagagacc ggcgcgggga
      121 tgatccacga ccccaccgcc ggcacgctga ccgtggtctg cgaggtcacc caccccgcct
      181 tcgtgctcct cgacccgggc gagcaggaac gacgggtcac gtcctggggc cgcgtgctcg
      241 ccaccgtgtg ccggtcggga cgcatcgcca ccttgcaggt gctggagcgg acgctgccgg
      301 actccggcac cggactcgcg gagtggtggg aagcccacgg cacggcggat gactcctggg
      361 ccgcacagac atatgcggag ctgatcgacc gcgccggccc ggccggggaa cggcacgcga
      421 ccacgctgtc gctggcgctg gacatgaacg cgagcgcgcg gcagatcagg accgccggcg
      481 gcggcctgcg cggtgccgcc gcggtgctgc gacaggagat gaacaccctg gtcgccgccc
      541 tgcgctcggc cgacctgtta ccgtccgggt ggctctcgcc gggacagatc gcggtgatcc
      601 tccgcagcgc gtatgacccg gcgatcgccg ccaccctcga acgccacggg cagcttggcc
      661 agtccctcgc caccgccggc ccggtcgccg tcaacgagtc ctgggcgcgg ctgcgcaccg
      721 actcggccca ccacgcggtg ctgtgggtct ccgaatggcc ccgctcgctg gtgtatccgg
      781 ggttcctgtc cccggtgctc ctctcgacgg ggattcagcg gtcgttctcg ttgctgtgca
      841 cgccgatgcg ctccgatcag gccgcccgcg acatccgcaa gaagaaggtc gagcacatct
      901 ccgaccaggc tcaacgggcg aagatcggac agatcgagga cgccgcccag accgcggagt
      961 accacgacgt gctacagcag gaagccgacc tcaccgccgg ccacggcgtg ctgcgctaca
     1021 ccggcctgat cgccgtctcc gcccccaccg tggaagaact cgacgcgggc gtggcggcga
     1081 tcgagcaggc cgcgatccag gcctcctgcg agacccgcct cctcgtcggg cagcaagccg
     1141 ccgcgttcac cgccgccgcg ctcccgctct gccgccgaat ctgacccggc cgccgcgcgt
     1201 gggagcgatg gtgcacctga ccggtggccc ccgaatcgcg ggggtactcg cccgcgtcca
     1261 gggaggccgt catgcccacg ttccacgacc cactcgccga tgccgccgaa gcatccgagg
     1321 cgatacgcgg tctcgcccac gcatcccgcg tattcgatgg cccggccgac acctacaccg
     1381 tgttcggcga cctcaccgcg gccatgcggt cgctgcggca ggtgctcgac cagctcgccg
     1441 ccacccacct cgcccaccgc gaacgcgcgc accacgacga cggcgaccac ctcgccggtg
     1501 atcgctcagc cctcgccgcc gcggacgagc tgcaccaggc cggcaccctg ctcgaccagg
     1561 cccacgacca cctcaacgcc gcgttctccc actcgggtcg catcgcctgg caccccgcac
     1621 ccgcgcaagc cgggctcgac cacgaccccg ccacgtccac cgcgacggcg gggacgctga
     1681 gactgacggt ctggcccgat gccccggtgg gtgagcacca gcgctacgcc taccgcatca
     1741 cggacaccac caccgggcag gagctggagg gacgggacct gttcaccggc gccggccagc
     1801 ccgtcggccc cgaccacgcg atccacgacc tcgcgacctt cctcggcgcc gccggtgaag
     1861 cccgtcagta cgccctcgac caccccgaca ccgagcccga gcacgcaggt ctgttcccga
     1921 gctgggtggc cgaggccgcc cgcaccaacg ccgacgccct caccgaactc accgaacccg
     1981 ccgaacggac ggctatagcc gaagcgtccc ggcctgagcg ccggtggatc aacgtggtgt
     2041 tcctgcaagg cgaggacgcc gacgaagtgc tggacctgat cgaccgggac ggcaccgccg
     2101 cgggcgtcga gcacctgaag ggctgggact acggggagga gaccacccag gcagcgctgg
     2161 agaacggcta cgtctacgac gagcccccga ccggcgcact ggacagggtg atcaccggcg
     2221 aagacggcta caccctcacc tacaacccgt tcgccggaca cgtcagcctc ctccgcgagt
     2281 acgcaccagc ctccgacgtc ctcgaagacg ccgtggtgcc ggcgcgggag gcgatcaccg
     2341 ggatcggcgc cccgcccgca cctcagccgg cgcaggcgtc cgccacgcgg cggacggccc
     2401 tcggcgcgga caaggactgg ttcgccggac ccgcccgcac gaccggatcg gccgggcgag
     2461 ggctgtccct gtgaccggca cggagcccga gcggctgcac acatccgtcc tggtcgcccc
     2521 tgccgccgaa cgccgccgac tgcgcaagca acgccgacag gccgccgccc gcctccaagc
     2581 cgaacaacgc gcagtcgaaa agggggcggc acgggcaaag gccaaagccg agaaggctga
     2641 gcggcgcgcg acgacctacc taccggccgc cggcgaaccc ggcccggcgg cactcaaatc
     2701 gccgggccgg ttccggctgc cccgccatca agacacgtcc gcgacgctgg ccggggcgta
     2761 tccgttcgtc gccgaaggcg gcctcggctc cgatggcgtg ttcgtcgggc aagacctcta
     2821 ctccggcgga tcgttcgtct acgacccctg ggtgctctac gcgcgcggga tcatcaccgc
     2881 acccaacatc gtgctggccg gcatcgttgg ctccggcaag tcctcactcg ccaagtccct
     2941 ctacacccgc tcgttgccgt tcgggcgccg cgtctacgtg cccggcgacc cgaagggcga
     3001 gcacaccgcc gtcgccgaag cggtcggcgg
//
LOCUS       DAOSUG010000394         3030 bp    DNA     linear   ENV 10-JUN-2024
DEFINITION  MAG TPA_asm: Micropruina sp. isolate AMR_MDS_2195
            NODE_13151_length_3030_cov_4.809412, whole genome shotgun sequence.
ACCESSION   DAOSUG010000394 DAOSUG010000000
VERSION     DAOSUG010000394.1
DBLINK      BioProject: PRJNA925477
            BioSample: SAMN32864262
            Sequence Read Archive: SRR4236673
KEYWORDS    WGS; ENV; Metagenome Assembled Genome; MAG; Third Party Data; TPA;
            TPA:assembly.
SOURCE      Micropruina sp. (wastewater metagenome)
  ORGANISM  Micropruina sp.
            Bacteria; Bacillati; Actinomycetota; Actinomycetes;
            Propionibacteriales; Nocardioidaceae; Micropruina.
REFERENCE   1  (bases 1 to 3030)
  AUTHORS   Abdulkadir,N., Saraiva,J.P., Zhang,J., Stolte,S., Gillor,O.,
            Harms,H. and Rocha,U.
  TITLE     Genome-centric analyses of 165 metagenomes show that mobile genetic
            elements are crucial for the transmission of antimicrobial
            resistance genes to pathogens in activated sludge and wastewater
  JOURNAL   Microbiol Spectr 12 (3), e0291823 (2024)
   PUBMED   38289113
REFERENCE   2  (bases 1 to 3030)
  AUTHORS   Abdulkadir,N.
  TITLE     Direct Submission
  JOURNAL   Submitted (24-MAR-2023) Environmental Microbiology, Helmholtz
            Center for Environmental Research -UFZ, Permoserstr 15, Leipzig
            04318, Germany
COMMENT     The annotation was added by the NCBI Prokaryotic Genome Annotation
            Pipeline (PGAP). Information about PGAP can be found here:
            https://www.ncbi.nlm.nih.gov/genome/annotation_prok/
            
            ##Genome-Assembly-Data-START##
            Assembly Method        :: SPAdes v. 3.11.1
            Genome Representation  :: Full
            Expected Final Version :: Yes
            Genome Coverage        :: Not Applicable
            Sequencing Technology  :: Illumina MiSeq
            ##Genome-Assembly-Data-END##
            
            ##Genome-Annotation-Data-START##
            Annotation Provider               :: NCBI
            Annotation Date                   :: 05/04/2023 04:57:10
            Annotation Pipeline               :: NCBI Prokaryotic Genome
                                                 Annotation Pipeline (PGAP)
            Annotation Method                 :: Best-placed reference protein
                                                 set; GeneMarkS-2+
            Annotation Software revision      :: 6.5
            Features Annotated                :: Gene; CDS; rRNA; tRNA; ncRNA
            Genes (total)                     :: 2,776
            CDSs (total)                      :: 2,750
            Genes (coding)                    :: 2,714
            CDSs (with protein)               :: 2,714
            Genes (RNA)                       :: 26
            tRNAs                             :: 24
            ncRNAs                            :: 2
            Pseudo Genes (total)              :: 36
            CDSs (without protein)            :: 36
            Pseudo Genes (ambiguous residues) :: 0 of 36
            Pseudo Genes (frameshifted)       :: 2 of 36
            Pseudo Genes (incomplete)         :: 32 of 36
            Pseudo Genes (internal stop)      :: 2 of 36
            ##Genome-Annotation-Data-END##
FEATURES             Location/Qualifiers
     source          1..3030
                     /organism="Micropruina sp."
                     /mol_type="genomic DNA"
                     /submitter_seqid="NODE_13151_length_3030_cov_4.809412"
                     /isolate="AMR_MDS_2195"
                     /isolation_source="Activated sludge of municipal
                     wastewater treatment plants"
                     /db_xref="taxon:2737536"
                     /environmental_sample
                     /geo_loc_name="Canada"
                     /lat_lon="49.25 N 123.1 W"
                     /collection_date="2015-06-16"
                     /metagenome_source="wastewater metagenome"
                     /note="metagenomic"
     gene            complement(<1..830)
                     /locus_tag="PLF56_11645"
     CDS             complement(<1..830)
                     /locus_tag="PLF56_11645"
                     /inference="COORDINATES: protein motif:HMM:NF019474.2"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_function: GO:0016787 - hydrolase activity [Evidence
                     IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="alpha/beta hydrolase"
                     /protein_id="HOQ54250.1"
                     /translation="MADPSWVEHGVPGRHGEVRVREYTPAGEPSGPRLVWVHGGGWVV
                     GTLSEPEAHAVGMAVAAAGFPVTSVEYGLVPSFPFVGPLRLKASPFRYPVPQDQVMDA
                     WQYAARRHGGAMWLGGASAGACLAAAVGLRLRDGEGLAPLGLAFVYGLFHGLLPDPSV
                     ALRRRLFGLSDLRVVQEMVRRAAVNHAGSAELLTNPHVFPGLADLTGLPPVQLLNADR
                     DMLRASAENFAHRLTQAGVPHDGWYLPGTTHGFLARPEQPPFTAGIAAMTGWLAGSST
                     T"
     gene            927..2063
                     /locus_tag="PLF56_11650"
     CDS             927..2063
                     /locus_tag="PLF56_11650"
                     /inference="COORDINATES: protein motif:HMM:NF022702.2"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="DUF3048 domain-containing protein"
                     /protein_id="HOQ54251.1"
                     /translation="MHELSRRTALVGLLGTAGAVAVGCTASPTPSTSASEAPSLTPTP
                     SPSPSVDDRPRWPLSGRLLKDEDAAKHAAVAVKVPDNKNEHPQRGLDQADIVFVELDG
                     YRDSSGYSSTRLVPVFHSSMPDAVGPVRSIRPVDIALLSPMHAIIGNTGAAPWVLNYV
                     KHNSSYVEGMLSYMNTKYSKIRGSYSIDQSRVRVLGGIKYFDRATVCHPTILAKQTKK
                     FRDGPPQVYFPFGDAKVASTVEGKTALSIKVPWKQGDSYDMSYTWSEKTGTWLRSMPW
                     GPHVLANGKRVAPANVLVIRAKQHYDKIYSGTGHDEPIHDIIKNSGAFHYFHGGKYVT
                     GTWNKGDIQDAFTFTLDDGSPLVMAPGQTYVELANKGAKITVKS"
     gene            complement(2166..>3030)
                     /locus_tag="PLF56_11655"
     CDS             complement(2166..>3030)
                     /locus_tag="PLF56_11655"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_015582083.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=2
                     /transl_table=11
                     /product="lyase family protein"
                     /protein_id="HOQ54252.1"
                     /translation="IKGPMGTAQDMLDLLGGSTKKQLALENQIGQYLGFARIFTSTGQ
                     VYPRSLDYEVVTALVQVAAAPSNVATTIRLMAGLELVTEGFKEGQVGSSAMPHKMNTR
                     SCERVNGLGVVLRGYATMIGELAGDQWNEGDVSCSVVRRIALPDAFYALDGLFETFLT
                     VLGEFGAFPAVIEAELARYLPFLTTTKVLMAAVRAGVGRETAHEAIKEHAVAVALDLR
                     RGARTNDLLDRLASDPRLGLTHEQLDATQADPLELTGAARDQVDDVADKVKRIAKQYP
                     DAAAYVPGSVL"
BASE COUNT          524 a         1054 c         1014 g          438 t
ORIGIN      
        1 gtcgtggacg acccagcgag ccagccggtc atcgccgcaa tgccggcggt gaacggcggc
       61 tgctcgggcc gggccagaaa accatgggtg gtgccgggca ggtaccagcc gtcgtgcggc
      121 accccggcct gcgtcaaccg gtgcgcgaag ttctcggccg aggcgcgcag catgtcgcga
      181 tcggcgttca gcaactggac gggaggcagc ccggtcaggt ccgcgagccc gggaaacacg
      241 tgcgggttgg tgagcaactc ggccgaaccg gcatggttga ccgcggcccg gcgcaccatt
      301 tcttgcacca cgcgcagatc cgagagcccg aacagccgtc gtcgcagggc caccgagggg
      361 tcgggcagca ggccgtggaa caacccgtac acgaaggcga gcccgagcgg ggccagcccc
      421 tcaccgtcgc gcaggcgaag tccgaccgcg gccgccaggc aggcaccggc ggaggctccc
      481 cccagccaca tcgcgccccc gtgccgacgg gcggcgtact gccaggcgtc catcacctga
      541 tcctggggaa ccgggtagcg gaagggcgag gccttcagcc gcagtgggcc gacgaacgga
      601 aagctcggca ccaggccgta ctcgacgctg gtgaccggaa agccggcggc cgcgacggcc
      661 atgccgacgg catgcgcctc gggttcgctg agcgtgccga ccacccaccc gccgccgtgc
      721 acccagacca gccggggtcc cgacggttcg ccggcggggg tgtactcgcg cacccgcacc
      781 tcaccgtgcc ggccgggaac gccgtgctcc acccaggacg gatccgccac attcacctcc
      841 gggtactggg ccgcggatgc ggcgcgctga ccatgatgcc agcgcttggc gcacccccgc
      901 tggggtgtgg ttgtacctta tgggacatgc acgaactgag ccgtcggacg gccctcgtcg
      961 gtctgctcgg taccgctggt gcggtcgcgg tcggatgcac cgcatcgccg accccttcca
     1021 ccagcgcgtc cgaggcgccg agcttaacgc ccacacccag cccgtccccg tcggtcgacg
     1081 accggccgcg gtggcccctg tcgggccggc tgctcaaaga cgaggacgcc gccaagcacg
     1141 cggcggtggc ggtgaaagtg cccgacaaca agaacgaaca cccgcagcgt gggctggacc
     1201 aggccgacat cgtcttcgtc gaactggacg ggtaccgcga ctcgtccggg tacagcagca
     1261 cgcgactggt gccggtgttc cactcctcga tgccggacgc ggtcgggccg gtgcggtcca
     1321 ttcgtccggt ggacatcgca ctgctcagcc cgatgcacgc gatcatcggc aacaccgggg
     1381 cggcgccgtg ggtgctcaac tacgtcaagc acaacagcag ttatgtcgag ggaatgctct
     1441 cgtacatgaa caccaagtac agcaagatcc ggggctcgta ctcgatcgac cagtcccggg
     1501 tgcgagtgct gggtggcatc aagtatttcg accgagccac ggtgtgtcac ccgacgattc
     1561 tggccaagca gaccaagaaa ttccgcgacg ggccgccgca ggtctacttt cccttcggcg
     1621 atgccaaggt ggccagcacg gtcgaaggca agaccgcgct ctcgatcaag gtgccctgga
     1681 agcagggcga cagctacgac atgagctaca cctggagcga gaagaccggc acctggttac
     1741 gtagtatgcc gtggggcccg cacgtgctgg ccaatggcaa gcgggtcgcc cccgccaacg
     1801 tgctggtgat ccgcgccaag cagcactacg acaagatcta ctccggaacc ggtcacgacg
     1861 aacctattca cgacatcatc aagaacagcg gcgcatttca ctatttccac ggcggcaagt
     1921 acgtcaccgg cacgtggaac aaaggtgaca ttcaagacgc cttcaccttc accctggacg
     1981 acggcagccc gctggtgatg gcccccgggc agacctatgt cgaactggcc aacaagggcg
     2041 cgaagatcac cgtcaagagc tgagcgggcc cccctccggc gaacccccgc ctcactatct
     2101 cgcgcctgcg aagcttcgca ggcgcgagat ggtgaggcgg gcgctcgggc ttcaggcgcc
     2161 cccccctaca gcaccgagcc gggcacgtag gccgccgcgt ccgggtactg cttggcgatg
     2221 cgcttgacct tgtcggccac gtcgtccacc tggtcgcggg cggcgccggt gagttccagc
     2281 gggtcggcct gggtggcgtc cagttgctcg tgggtgaggc cgagccgcgg atcggacgcc
     2341 agccggtcga gcaggtcgtt ggtgcgggcc ccgcggcgca ggtcgagcgc taccgccacc
     2401 gcgtgttcct tgatcgcctc gtgcgcggtc tcgcgtccga cgccggcgcg gacggccgcc
     2461 atcagcacct tcgtggtggt caggaacggc aggtaccgcg ccagttccgc ctcgatcacg
     2521 gccggaaagg cgccgaactc gcccaacacg gtgaggaagg tctcaaatag tccatcgagg
     2581 gcgtagaagg cgtccggcag ggcgatccgg cgcaccaccg aacacgacac gtcgccctcg
     2641 ttccactggt cgccggccag ctcgccgatc atcgtggcgt agccgcgcag caccacgccg
     2701 aggccgttga cgcgttcaca tgagcgggtg ttcatcttgt gcggcatcgc cgaggatccg
     2761 acctgcccct ccttgaagcc ctcggtgacg agttcgaggc ccgccatcag ccggatcgtc
     2821 gtcgccacgt tggacggggc cgccgccacc tgaaccagcg ccgtcaccac ctcgtagtcg
     2881 agtgaacgcg ggtacacctg gccggttgag gtgaagatgc gcgcgaagcc gaggtactga
     2941 ccgatctggt tctcgagcgc caactgcttc ttggtgctgc cgccgagcag gtcgagcatg
     3001 tcctgcgcgg tgcccatcgg gcccttgatg
//
LOCUS       DAOSUG010000395         3023 bp    DNA     linear   ENV 10-JUN-2024
DEFINITION  MAG TPA_asm: Micropruina sp. isolate AMR_MDS_2195
            NODE_13186_length_3023_cov_2.857480, whole genome shotgun sequence.
ACCESSION   DAOSUG010000395 DAOSUG010000000
VERSION     DAOSUG010000395.1
DBLINK      BioProject: PRJNA925477
            BioSample: SAMN32864262
            Sequence Read Archive: SRR4236673
KEYWORDS    WGS; ENV; Metagenome Assembled Genome; MAG; Third Party Data; TPA;
            TPA:assembly.
SOURCE      Micropruina sp. (wastewater metagenome)
  ORGANISM  Micropruina sp.
            Bacteria; Bacillati; Actinomycetota; Actinomycetes;
            Propionibacteriales; Nocardioidaceae; Micropruina.
REFERENCE   1  (bases 1 to 3023)
  AUTHORS   Abdulkadir,N., Saraiva,J.P., Zhang,J., Stolte,S., Gillor,O.,
            Harms,H. and Rocha,U.
  TITLE     Genome-centric analyses of 165 metagenomes show that mobile genetic
            elements are crucial for the transmission of antimicrobial
            resistance genes to pathogens in activated sludge and wastewater
  JOURNAL   Microbiol Spectr 12 (3), e0291823 (2024)
   PUBMED   38289113
REFERENCE   2  (bases 1 to 3023)
  AUTHORS   Abdulkadir,N.
  TITLE     Direct Submission
  JOURNAL   Submitted (24-MAR-2023) Environmental Microbiology, Helmholtz
            Center for Environmental Research -UFZ, Permoserstr 15, Leipzig
            04318, Germany
COMMENT     The annotation was added by the NCBI Prokaryotic Genome Annotation
            Pipeline (PGAP). Information about PGAP can be found here:
            https://www.ncbi.nlm.nih.gov/genome/annotation_prok/
            
            ##Genome-Assembly-Data-START##
            Assembly Method        :: SPAdes v. 3.11.1
            Genome Representation  :: Full
            Expected Final Version :: Yes
            Genome Coverage        :: Not Applicable
            Sequencing Technology  :: Illumina MiSeq
            ##Genome-Assembly-Data-END##
            
            ##Genome-Annotation-Data-START##
            Annotation Provider               :: NCBI
            Annotation Date                   :: 05/04/2023 04:57:10
            Annotation Pipeline               :: NCBI Prokaryotic Genome
                                                 Annotation Pipeline (PGAP)
            Annotation Method                 :: Best-placed reference protein
                                                 set; GeneMarkS-2+
            Annotation Software revision      :: 6.5
            Features Annotated                :: Gene; CDS; rRNA; tRNA; ncRNA
            Genes (total)                     :: 2,776
            CDSs (total)                      :: 2,750
            Genes (coding)                    :: 2,714
            CDSs (with protein)               :: 2,714
            Genes (RNA)                       :: 26
            tRNAs                             :: 24
            ncRNAs                            :: 2
            Pseudo Genes (total)              :: 36
            CDSs (without protein)            :: 36
            Pseudo Genes (ambiguous residues) :: 0 of 36
            Pseudo Genes (frameshifted)       :: 2 of 36
            Pseudo Genes (incomplete)         :: 32 of 36
            Pseudo Genes (internal stop)      :: 2 of 36
            ##Genome-Annotation-Data-END##
FEATURES             Location/Qualifiers
     source          1..3023
                     /organism="Micropruina sp."
                     /mol_type="genomic DNA"
                     /submitter_seqid="NODE_13186_length_3023_cov_2.857480"
                     /isolate="AMR_MDS_2195"
                     /isolation_source="Activated sludge of municipal
                     wastewater treatment plants"
                     /db_xref="taxon:2737536"
                     /environmental_sample
                     /geo_loc_name="Canada"
                     /lat_lon="49.25 N 123.1 W"
                     /collection_date="2015-06-16"
                     /metagenome_source="wastewater metagenome"
                     /note="metagenomic"
     gene            <1..574
                     /locus_tag="PLF56_11660"
     CDS             <1..574
                     /locus_tag="PLF56_11660"
                     /inference="COORDINATES: protein motif:HMM:NF012894.2"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=2
                     /transl_table=11
                     /product="OmpA family protein"
                     /protein_id="HOQ54253.1"
                     /translation="REVSFVINPVAGEQVKLSQLQAGYLHAVWEGLAKAAGAKRVEFF
                     DGTGTTAGQGVGPVVQVPQPDDVSTDATTTEVVCTLPTPALFVINTPNLIDRDKTLQG
                     LKKCLAKAPKTYRVVVEGRTSGDPSDNGKATVELSKQRATVVAVLLKELKVPAKAIYK
                     VVGYGRSKPLVQPPTDSRNRAVVVRFEVVG"
     gene            764..2491
                     /locus_tag="PLF56_11665"
     CDS             764..2491
                     /locus_tag="PLF56_11665"
                     /EC_number="1.1.1.38"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_014847554.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_function: GO:0004470 - malic enzyme activity [Evidence
                     IEA];
                     GO_function: GO:0004471 - malate dehydrogenase
                     (decarboxylating) (NAD+) activity [Evidence IEA];
                     GO_function: GO:0046872 - metal ion binding [Evidence
                     IEA];
                     GO_function: GO:0051287 - NAD binding [Evidence IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="NAD-dependent malic enzyme"
                     /protein_id="HOQ54254.1"
                     /translation="MPVADLYLDTTNSRDVVLQVRARGRQILRNPLANRGTAFTLQER
                     ERLDLSGLLPSRVTTIEEQLRRTYAQYKRSPSPLAKFIYLSQLRDRNEVLFYRLLSEN
                     IEELLPIIYTPTIGEAIERFSHEYTSSRGLYLSIDRPDLIEQSLANYVPDPDEIDLVV
                     VTDSEGILGIGDQGIGGIQIANGKLSVYTAAAGIHPRRAIPIVLDVGTDNLGLLNSDL
                     YLGERHARVRGEAYDAFIESFVVAITRQFPNAMLHWEDFGAGNAHRILQRYADSICTF
                     NDDIQGTAAVVLAAVLAAVRLTGVPLPQHRVLIYGAGTAGVGIADIIRAAMGHEGLPL
                     NEAFGQFYAFNSRGLIVDDGDGIRDYQQPYARTRAEVDGWDVADPHRVSLAEVVRQVK
                     PTILIGTSAQPGAFNAEVIEAMAEGCDRPIIMPLSNPTSRSEASPADLLDWTNGRALI
                     ATGSPYGTMRHADVYHTIAQANNALIFPGLGLGVSVVRAERVSNEMIYAAAAALAGLV
                     NEYRPGASLLPSMNDLRLVSATVAMAVAKTAESQGLARTPLTNPIDDIYQRMWKPQYP
                     RLVVVAADD"
     gene            complement(2508..2678)
                     /locus_tag="PLF56_11670"
     CDS             complement(2508..2678)
                     /locus_tag="PLF56_11670"
                     /inference="COORDINATES: ab initio
                     prediction:GeneMarkS-2+"
                     /note="Derived by automated computational analysis using
                     gene prediction method: GeneMarkS-2+."
                     /codon_start=1
                     /transl_table=11
                     /product="hypothetical protein"
                     /protein_id="HOQ54255.1"
                     /translation="MGVRSGRVARRYLSLDQAIMFGAPVNVLADGALQQWFTAGEEGD
                     VLRPLIAAEPAL"
BASE COUNT          506 a         1078 c         1016 g          423 t
ORIGIN      
        1 ccgtgaagtc agcttcgtga tcaacccggt ggccggcgag caggtcaagc tgagccagct
       61 gcaggccggg tacctgcatg cggtgtggga gggtctcgcc aaggccgccg gcgcgaaacg
      121 ggtcgagttc ttcgacggca ccggcaccac ggccgggcag ggcgtagggc cggtcgttca
      181 ggttccgcag ccggacgacg tcagcaccga cgccaccacg accgaagtgg tctgcacact
      241 gcccaccccg gccctgttcg tgatcaacac gcccaacctg atcgaccgcg acaagaccct
      301 gcaggggctc aagaagtgcc tggccaaggc ccccaagacc taccgcgtcg tggtcgaggg
      361 acgcacctcg ggcgacccgt ccgacaacgg caaggccacc gtcgagctct ccaaacaacg
      421 agcaacggtg gtcgcggtgc tgctcaagga gctgaaggtg cccgccaagg ccatctacaa
      481 ggtggtcgga tacggacgca gcaagccgct ggtgcagccg ccgaccgact cccgcaaccg
      541 tgctgtggtg gtgcggttcg aggtggtggg ctgaggtccg ggagctgccg gctcgcgggg
      601 cggacgtgat cgccccgcga cgccgtccgg cacccggtgc cgccacgctg gacgaccggc
      661 gcccggacgc gggcccgcac gtcgcgtccg gtccacgatc agctgcttgc ctgctggggc
      721 cacagcctag gcttcgagcc accgccgacg acgaaggact cccatgccag tagccgacct
      781 gtacctcgac accaccaaca gccgagatgt ggtgctgcag gtgcgggcgc ggggcaggca
      841 gattctgcgc aacccgctgg cgaaccgtgg caccgccttc accctgcagg agcgtgaacg
      901 actcgacctg tcgggcctgc tgcccagccg cgtgaccacc atcgaggagc agctgcggcg
      961 cacctacgcc cagtacaagc ggtcaccctc gccgctggcg aagttcatct acctgtccca
     1021 gctgcgcgac cgcaacgagg tgctgttcta ccggctgctc agcgagaaca tcgaagagct
     1081 gctgccgatc atctacacgc cgaccatcgg cgaagcgatc gagcggttca gccacgagta
     1141 caccagttca cgcgggctgt acctgtcgat cgaccggccc gacctgatcg aacagtcgct
     1201 ggccaactac gtgcccgacc ccgacgagat cgacctggtg gtggtcaccg actccgaggg
     1261 catcctgggc atcggtgacc agggcatcgg cggcattcag atcgcgaacg gcaagctgag
     1321 cgtgtacacc gcggcggccg gcattcaccc gcgccgggcg atcccgatcg tgctggacgt
     1381 gggcaccgac aacctcggcc tgctgaacag cgatctgtat ctgggtgagc ggcacgcccg
     1441 ggtgcgcggc gaggcctacg acgcgttcat cgaatcgttc gtggtggcga tcacccggca
     1501 gttccccaac gcgatgctgc actgggagga cttcggcgcc ggcaacgcgc accgcatcct
     1561 gcagcgctac gccgactcga tctgcacctt caacgacgac atccagggca cggccgcggt
     1621 ggtgctggcc gcggtactgg ccgccgtccg gctgaccggg gtgccgttgc cgcagcatcg
     1681 ggtgctgatc tacggcgccg gcaccgcagg ggtgggcatc gccgacatca tccgtgcggc
     1741 gatggggcac gaagggctac ccctgaacga ggcgttcggc cagttctacg ccttcaacag
     1801 ccgtgggctg atcgtcgacg acggcgacgg catccgcgac taccagcagc cctacgcccg
     1861 cacccgtgcc gaggtggacg ggtgggacgt ggcggatccg caccgggtca gcctggccga
     1921 ggtggtgcgg caggtgaagc cgacgatact gatcggcacc tcggcccagc ccggagcgtt
     1981 caacgccgag gtgatcgagg cgatggcaga agggtgcgat cgtccgatca tcatgccgtt
     2041 gtcgaacccg accagccgct cggaggcgag cccggccgac ctgctcgact ggacgaacgg
     2101 gcgcgcgctg atcgccaccg ggagccccta cggcaccatg cggcacgcgg acgtctacca
     2161 caccatcgcg caggccaaca acgcgctgat ctttccgggt ctgggcctgg gcgtgtccgt
     2221 ggtgcgggcc gaacgggtca gcaacgagat gatctacgcc gcggccgccg cgctggccgg
     2281 cctggtcaac gagtaccgtc cgggcgcctc gctgctgccg tccatgaacg acctgcggct
     2341 ggtgtcggcc acggtcgcga tggcggtggc gaagaccgcc gagagccagg gcctggcccg
     2401 cacgccgttg accaatccga tcgacgacat ctaccagcgc atgtggaagc cgcagtaccc
     2461 gcggctcgtt gtggtggccg ccgacgactg accgcgggtg cgcgacctca cagcgcgggt
     2521 tccgcagcga tcagcggacg caacacgtcc ccttcctcgc ccgcggtgaa ccactgctgc
     2581 agcgcaccgt cggccaacac gttcaccggg gcgccgaaca ttatcgcctg gtcgaggctg
     2641 aggtagcggc gggcgacccg ccccgaccgc acacccaccg cgtcgcagaa gccgcccggc
     2701 ccgtagcagc ccaggtcccg ctcgagcgag gcgccggacc gatcaccctg gccgcccccg
     2761 catctcccga ctgaagatct gccgagctat cgctggtcgg accagctctc tcgtctccgg
     2821 gccggccccg ggatctccac gcaggcacag ctccacggag gcgcccggac gacgggcgtc
     2881 accgttgcgt tgcgcggctc gtcgggggga tggaccgatc agccggcggt ccgatgccag
     2941 gtccagccca gccggcccag ttcgtccgcg atcgaccgga tcgccttcgg ccctaccccg
     3001 gggagtgcca ggagttcgcg ctc
//
LOCUS       DAOSUG010000396         3016 bp    DNA     linear   ENV 10-JUN-2024
DEFINITION  MAG TPA_asm: Micropruina sp. isolate AMR_MDS_2195
            NODE_13230_length_3016_cov_4.276596, whole genome shotgun sequence.
ACCESSION   DAOSUG010000396 DAOSUG010000000
VERSION     DAOSUG010000396.1
DBLINK      BioProject: PRJNA925477
            BioSample: SAMN32864262
            Sequence Read Archive: SRR4236673
KEYWORDS    WGS; ENV; Metagenome Assembled Genome; MAG; Third Party Data; TPA;
            TPA:assembly.
SOURCE      Micropruina sp. (wastewater metagenome)
  ORGANISM  Micropruina sp.
            Bacteria; Bacillati; Actinomycetota; Actinomycetes;
            Propionibacteriales; Nocardioidaceae; Micropruina.
REFERENCE   1  (bases 1 to 3016)
  AUTHORS   Abdulkadir,N., Saraiva,J.P., Zhang,J., Stolte,S., Gillor,O.,
            Harms,H. and Rocha,U.
  TITLE     Genome-centric analyses of 165 metagenomes show that mobile genetic
            elements are crucial for the transmission of antimicrobial
            resistance genes to pathogens in activated sludge and wastewater
  JOURNAL   Microbiol Spectr 12 (3), e0291823 (2024)
   PUBMED   38289113
REFERENCE   2  (bases 1 to 3016)
  AUTHORS   Abdulkadir,N.
  TITLE     Direct Submission
  JOURNAL   Submitted (24-MAR-2023) Environmental Microbiology, Helmholtz
            Center for Environmental Research -UFZ, Permoserstr 15, Leipzig
            04318, Germany
COMMENT     The annotation was added by the NCBI Prokaryotic Genome Annotation
            Pipeline (PGAP). Information about PGAP can be found here:
            https://www.ncbi.nlm.nih.gov/genome/annotation_prok/
            
            ##Genome-Assembly-Data-START##
            Assembly Method        :: SPAdes v. 3.11.1
            Genome Representation  :: Full
            Expected Final Version :: Yes
            Genome Coverage        :: Not Applicable
            Sequencing Technology  :: Illumina MiSeq
            ##Genome-Assembly-Data-END##
            
            ##Genome-Annotation-Data-START##
            Annotation Provider               :: NCBI
            Annotation Date                   :: 05/04/2023 04:57:10
            Annotation Pipeline               :: NCBI Prokaryotic Genome
                                                 Annotation Pipeline (PGAP)
            Annotation Method                 :: Best-placed reference protein
                                                 set; GeneMarkS-2+
            Annotation Software revision      :: 6.5
            Features Annotated                :: Gene; CDS; rRNA; tRNA; ncRNA
            Genes (total)                     :: 2,776
            CDSs (total)                      :: 2,750
            Genes (coding)                    :: 2,714
            CDSs (with protein)               :: 2,714
            Genes (RNA)                       :: 26
            tRNAs                             :: 24
            ncRNAs                            :: 2
            Pseudo Genes (total)              :: 36
            CDSs (without protein)            :: 36
            Pseudo Genes (ambiguous residues) :: 0 of 36
            Pseudo Genes (frameshifted)       :: 2 of 36
            Pseudo Genes (incomplete)         :: 32 of 36
            Pseudo Genes (internal stop)      :: 2 of 36
            ##Genome-Annotation-Data-END##
FEATURES             Location/Qualifiers
     source          1..3016
                     /organism="Micropruina sp."
                     /mol_type="genomic DNA"
                     /submitter_seqid="NODE_13230_length_3016_cov_4.276596"
                     /isolate="AMR_MDS_2195"
                     /isolation_source="Activated sludge of municipal
                     wastewater treatment plants"
                     /db_xref="taxon:2737536"
                     /environmental_sample
                     /geo_loc_name="Canada"
                     /lat_lon="49.25 N 123.1 W"
                     /collection_date="2015-06-16"
                     /metagenome_source="wastewater metagenome"
                     /note="metagenomic"
     gene            124..456
                     /locus_tag="PLF56_11675"
     CDS             124..456
                     /locus_tag="PLF56_11675"
                     /inference="COORDINATES: protein motif:HMM:NF013698.2"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_function: GO:0003677 - DNA binding [Evidence IEA];
                     GO_function: GO:0004803 - transposase activity [Evidence
                     IEA];
                     GO_process: GO:0006313 - transposition, DNA-mediated
                     [Evidence IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="transposase"
                     /protein_id="HOQ54256.1"
                     /translation="MTPPEVIGGADTHTDTLHVALITTLGQQLADHEFPTTAAGYTAA
                     ITFLTGQGALQRVGIEGTSSYGAGLATACLTAGIEVVEVNSPSRGDRRRQGKSDDDAR
                     LNLPRPAR"
     gene            662..1081
                     /locus_tag="PLF56_11680"
     CDS             662..1081
                     /locus_tag="PLF56_11680"
                     /inference="COORDINATES: ab initio
                     prediction:GeneMarkS-2+"
                     /note="Derived by automated computational analysis using
                     gene prediction method: GeneMarkS-2+."
                     /codon_start=1
                     /transl_table=11
                     /product="hypothetical protein"
                     /protein_id="HOQ54257.1"
                     /translation="MPAFLPAVCERGMTGDGHVLELAERAAEDVRELNHRTRDPRVFT
                     APAEMYRLVGELAVLAGGLPQLLGQLDRWLRAEHDHGRIRADTGTGPGRIVAAAGADL
                     AGAGDAADELANALDAALAHLAHLGAADSGHAVSKRG"
     gene            1168..2502
                     /locus_tag="PLF56_11685"
     CDS             1168..2502
                     /locus_tag="PLF56_11685"
                     /inference="COORDINATES: ab initio
                     prediction:GeneMarkS-2+"
                     /note="Derived by automated computational analysis using
                     gene prediction method: GeneMarkS-2+."
                     /codon_start=1
                     /transl_table=11
                     /product="DDE-type integrase/transposase/recombinase"
                     /protein_id="HOQ54258.1"
                     /translation="MLTQEEDVDAHALRRRGWSISAIARHLGKDRKTIRAYLNGERTA
                     GVRSPAGPDVFEPFVTYCRERLVEDPHLWATALYDELLQLGYDRSYPRLTHNLRTRGL
                     RPVCHACRPAGGRPAAVIDHPPAEETQWDWLELPDPPRSWDGYGAKAFLLVGALAHSS
                     KWRGVLAEAMDQPQLIDAQHQVVVRLGGLTRVWRFDRMATVVHPGTGKVTASYAAVAK
                     HYGVQVKPCPPRRGNRKGVVEKANHTAAQRWWRTLPDDITVADAQARLDQFCATVGDT
                     RDRVDVDGNRCTVADLAARERLAPLPATAFPAELAVERVVSAQALVSFRGNRYSVPPE
                     LANGPVTVTHRLGSPVLAFVTDRGVTVALHHRAGDGTGATIRSEHHVTALNKAALAAF
                     TTAKPHRRKQRIPPGPAARHAAQVLRGATTTPDPVVDLTVYAAAAARRRNLP"
     gene            2499..>3016
                     /locus_tag="PLF56_11690"
     CDS             2499..>3016
                     /locus_tag="PLF56_11690"
                     /inference="COORDINATES: protein motif:HMM:NF013828.2"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_function: GO:0005524 - ATP binding [Evidence IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="ATP-binding protein"
                     /protein_id="HOQ54259.1"
                     /translation="MTTTTTDDAPPPATPAENSRYQQLRAHLTTLKLLDAAAALPAVL
                     DQARAENLTMTAALERLLRIEVTATEARRLAGRLRFACLPTDATLDEFDYDAQPGVDP
                     RLVNDLASCRYLDNATNVLMIGPPGVGKTMLATGLARKAVEAGYRTYFTTAADLAARC
                     HRAAIEGRWATT"
BASE COUNT          471 a         1174 c          990 g          381 t
ORIGIN      
        1 cccgggctgc cgggctggtt ggacatgacc tcataggggc ctggcctcac ctgcttgatc
       61 attgtcctgt ctgaccagcc ggcctggtag ccgcaaacga caacggatag gacacctgct
      121 ctgatgacac caccggaggt gatcggcggc gccgacaccc acaccgacac actgcacgtc
      181 gccctgatca ccacccttgg ccagcagctc gccgaccacg agttcccgac caccgccgcc
      241 ggctacacag cggcgatcac ctttctgacc ggccagggcg ctctgcagcg agtagggatt
      301 gagggcacca gctcctacgg cgcgggcctg gccaccgcct gcctgactgc cgggatcgag
      361 gtggtggagg tgaacagtcc cagccgaggt gaccgccgcc ggcagggcaa gtccgatgat
      421 gacgctcgtt tgaatctccc cagacctgct cgctgaatct ccccacccct gtgaccccgc
      481 ttcatcgctc acatgggagg aacgaaatgc acgcacccgc atcaccccag ggtggctggt
      541 cgatcccggc ggctggccgc cacctcgcga cgaccgaccg gcgaccccgc gcagactcgg
      601 acggcgggcg gaccgccagc gcctgcccgc cgtgcggcac ccggccgcga tcgcatcgct
      661 cgtgcctgcg ttcctgccgg cggtctgtga gcgcggcatg acgggcgacg ggcacgtcct
      721 ggagttggcc gagcgggcgg ccgaggacgt gcgagagctc aaccaccgca cccgcgaccc
      781 acgcgtgttc accgctccgg ccgagatgta ccggttagtc ggcgagctcg ccgtgctggc
      841 cggcgggctg ccgcagctgc tgggccagct cgaccggtgg ctgcgcgcgg aacacgacca
      901 cggccggatc cgggccgaca ccggcaccgg gcccggccgg atcgtcgcgg ccgcgggcgc
      961 cgatctggcc ggcgccggcg acgccgcgga cgagttggcc aatgcgttgg acgccgcact
     1021 tgcgcacctg gcccacctgg gcgccgcgga ttccggccat gccgtttcga agcgcggttg
     1081 acgcgcgtgg ctccgcggaa gacggcgggg ctccctcgac gctggcggtc tctgaccacc
     1141 ggccagctgt tcgagggagc ccgtttcatg ctcacacagg aggaagacgt ggacgcgcac
     1201 gccctgcgcc gcagggggtg gtcgatctcc gcgatcgccc gccacctggg caaagaccga
     1261 aaaacgatca gggcgtactt gaacggtgag cggaccgccg gagtccgctc accggccggc
     1321 ccggacgtgt tcgaaccgtt cgtgacctac tgccgggaac ggctggtcga ggacccgcac
     1381 ctatgggcga ccgccttgta cgacgagttg ctgcagctgg gttacgaccg gtcctacccg
     1441 cggctgacgc acaacctgcg cactcgtggg ctgcggccgg tgtgccacgc ctgccggccg
     1501 gccggcggcc gccccgcagc ggtcatcgac cacccgccgg ccgaggaaac ccaatgggac
     1561 tggctcgagc tgcccgaccc acccaggagt tgggacggct acggagccaa ggcgttcctg
     1621 ctggtcggcg cgctggccca ctcgagcaag tggcgggggg tgctggccga ggcgatggac
     1681 cagccgcagc tgatcgacgc gcagcaccag gtcgtggtcc ggctcggcgg gctgacccgg
     1741 gtctggcggt tcgaccggat ggccaccgtc gtgcacccgg gcaccggcaa ggtcaccgcc
     1801 tcttacgccg cggtcgccaa gcactatggg gtacaggtca aaccgtgccc accgcggcgg
     1861 ggcaaccgca agggtgtggt cgagaaggcc aaccacaccg cggcgcaacg atggtggcgc
     1921 accctgcccg acgacatcac cgtcgccgac gcgcaggccc ggctcgacca gttctgcgcc
     1981 accgtcggcg acacccgcga ccgcgtcgat gtcgacggga accggtgcac cgtcgcggac
     2041 ctggccgccc gggaacggct cgccccgcta cccgccacgg cgttccccgc cgagctggcc
     2101 gtcgagcggg tcgtatcagc gcaggcgttg gtctcgttcc gcgggaaccg gtactccgtg
     2161 ccgcccgagc tggccaacgg gccggtcacc gtgacccacc gcctcggctc cccggtgctg
     2221 gcgttcgtca ccgaccgcgg cgtgacagta gccctgcatc accgggccgg cgacggcacc
     2281 ggcgccacta tccgcagcga gcaccacgtc accgcactga acaaagccgc gctggccgcg
     2341 ttcaccaccg ccaaaccgca ccgccgcaaa cagcgcatcc cacccggccc ggccgcccgg
     2401 cacgccgcac aggtgttgcg cggcgccacc accacacccg accccgtcgt cgatctgacc
     2461 gtctacgcgg ccgcggccgc ccgccggagg aacctgccat gaccaccacc accacggacg
     2521 acgcgccgcc gccggccacc ccggccgaga acagccgcta ccaacagctc cgcgcccacc
     2581 tgaccacgct gaagctgctc gacgcggccg cggccctgcc ggccgtcctg gaccaggccc
     2641 gcgccgagaa cctcaccatg accgcggctc tggaacggct gctgcggatc gaggtcaccg
     2701 cgaccgaggc acgccggctc gccggccggc tccggttcgc ctgcctgccc accgacgcca
     2761 ccctggacga gttcgactac gacgcccaac ccggcgtcga cccccggttg gtcaacgacc
     2821 tcgcctcctg ccgctacctc gacaacgcca ccaacgtcct gatgatcggg ccgcccggcg
     2881 tcgggaagac catgctcgcc accggtctgg cccgcaaagc cgtcgaggcc ggctaccgga
     2941 cctacttcac caccgccgcc gacctggccg cacgctgcca ccgggccgcg atcgaaggac
     3001 gatgggccac caccat
//
LOCUS       DAOSUG010000397         2994 bp    DNA     linear   ENV 10-JUN-2024
DEFINITION  MAG TPA_asm: Micropruina sp. isolate AMR_MDS_2195
            NODE_13355_length_2994_cov_4.704661, whole genome shotgun sequence.
ACCESSION   DAOSUG010000397 DAOSUG010000000
VERSION     DAOSUG010000397.1
DBLINK      BioProject: PRJNA925477
            BioSample: SAMN32864262
            Sequence Read Archive: SRR4236673
KEYWORDS    WGS; ENV; Metagenome Assembled Genome; MAG; Third Party Data; TPA;
            TPA:assembly.
SOURCE      Micropruina sp. (wastewater metagenome)
  ORGANISM  Micropruina sp.
            Bacteria; Bacillati; Actinomycetota; Actinomycetes;
            Propionibacteriales; Nocardioidaceae; Micropruina.
REFERENCE   1  (bases 1 to 2994)
  AUTHORS   Abdulkadir,N., Saraiva,J.P., Zhang,J., Stolte,S., Gillor,O.,
            Harms,H. and Rocha,U.
  TITLE     Genome-centric analyses of 165 metagenomes show that mobile genetic
            elements are crucial for the transmission of antimicrobial
            resistance genes to pathogens in activated sludge and wastewater
  JOURNAL   Microbiol Spectr 12 (3), e0291823 (2024)
   PUBMED   38289113
REFERENCE   2  (bases 1 to 2994)
  AUTHORS   Abdulkadir,N.
  TITLE     Direct Submission
  JOURNAL   Submitted (24-MAR-2023) Environmental Microbiology, Helmholtz
            Center for Environmental Research -UFZ, Permoserstr 15, Leipzig
            04318, Germany
COMMENT     The annotation was added by the NCBI Prokaryotic Genome Annotation
            Pipeline (PGAP). Information about PGAP can be found here:
            https://www.ncbi.nlm.nih.gov/genome/annotation_prok/
            
            ##Genome-Assembly-Data-START##
            Assembly Method        :: SPAdes v. 3.11.1
            Genome Representation  :: Full
            Expected Final Version :: Yes
            Genome Coverage        :: Not Applicable
            Sequencing Technology  :: Illumina MiSeq
            ##Genome-Assembly-Data-END##
            
            ##Genome-Annotation-Data-START##
            Annotation Provider               :: NCBI
            Annotation Date                   :: 05/04/2023 04:57:10
            Annotation Pipeline               :: NCBI Prokaryotic Genome
                                                 Annotation Pipeline (PGAP)
            Annotation Method                 :: Best-placed reference protein
                                                 set; GeneMarkS-2+
            Annotation Software revision      :: 6.5
            Features Annotated                :: Gene; CDS; rRNA; tRNA; ncRNA
            Genes (total)                     :: 2,776
            CDSs (total)                      :: 2,750
            Genes (coding)                    :: 2,714
            CDSs (with protein)               :: 2,714
            Genes (RNA)                       :: 26
            tRNAs                             :: 24
            ncRNAs                            :: 2
            Pseudo Genes (total)              :: 36
            CDSs (without protein)            :: 36
            Pseudo Genes (ambiguous residues) :: 0 of 36
            Pseudo Genes (frameshifted)       :: 2 of 36
            Pseudo Genes (incomplete)         :: 32 of 36
            Pseudo Genes (internal stop)      :: 2 of 36
            ##Genome-Annotation-Data-END##
FEATURES             Location/Qualifiers
     source          1..2994
                     /organism="Micropruina sp."
                     /mol_type="genomic DNA"
                     /submitter_seqid="NODE_13355_length_2994_cov_4.704661"
                     /isolate="AMR_MDS_2195"
                     /isolation_source="Activated sludge of municipal
                     wastewater treatment plants"
                     /db_xref="taxon:2737536"
                     /environmental_sample
                     /geo_loc_name="Canada"
                     /lat_lon="49.25 N 123.1 W"
                     /collection_date="2015-06-16"
                     /metagenome_source="wastewater metagenome"
                     /note="metagenomic"
     gene            <1..471
                     /gene="era"
                     /locus_tag="PLF56_11695"
     CDS             <1..471
                     /gene="era"
                     /locus_tag="PLF56_11695"
                     /EC_number="3.6.5.-"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_014846736.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_function: GO:0003924 - GTPase activity [Evidence IEA];
                     GO_function: GO:0005525 - GTP binding [Evidence IEA];
                     GO_function: GO:0019843 - rRNA binding [Evidence IEA];
                     GO_function: GO:0043022 - ribosome binding [Evidence IEA];
                     GO_process: GO:0042254 - ribosome biogenesis [Evidence
                     IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="GTPase Era"
                     /protein_id="HOQ54260.1"
                     /translation="RWAEIVPVSALTNDQVGLVADVLIAQLPEGPAYYPEGEVTDEPT
                     ETLIAELIREAALEKVRDELPHSITVTIDEMGLREGRPEDRPLLDVYASLVVERDSQK
                     GIVIGHQGSVLREIGTAARTQLTALLGTPVHLDLKVKVLKDWQRNAKYLNRLGF"
     gene            1020..1514
                     /locus_tag="PLF56_11700"
     CDS             1020..1514
                     /locus_tag="PLF56_11700"
                     /inference="COORDINATES: protein motif:HMM:NF020907.2"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="2-oxo-4-hydroxy-4-carboxy-5-ureidoimidazoline
                     decarboxylase"
                     /protein_id="HOQ54261.1"
                     /translation="MPSGGALRLAEFNGLPATAAHEIVLRCVSIKPWATALVARRPYR
                     DRQRLLATARELTDAWTWADVEAALDAWPFLDARAEGARVPAGVVVAGAEGTLSADLS
                     AVSEAYQERFGRSLVVGASGRTTSDVLRLAVRRLLMDEAGDRSATTAELRQISLRRLR
                     RMIL"
     gene            complement(1554..1931)
                     /locus_tag="PLF56_11705"
     CDS             complement(1554..1931)
                     /locus_tag="PLF56_11705"
                     /inference="COORDINATES: ab initio
                     prediction:GeneMarkS-2+"
                     /note="Derived by automated computational analysis using
                     gene prediction method: GeneMarkS-2+."
                     /codon_start=1
                     /transl_table=11
                     /product="hypothetical protein"
                     /protein_id="HOQ54262.1"
                     /translation="MRRLKAFSGVTPGAFVAASAIEGGAGRPRLGQLERLRQAWNELL
                     GDRVFVLPSTAGAAPRLWAAPHAVDAHRRATMRLTCLAGITGRPAVSAPLLQVPEGPL
                     GVCLVGPPGSDLALIELAGGLEG"
     gene            complement(2103..2522)
                     /locus_tag="PLF56_11710"
     CDS             complement(2103..2522)
                     /locus_tag="PLF56_11710"
                     /inference="COORDINATES: ab initio
                     prediction:GeneMarkS-2+"
                     /note="Derived by automated computational analysis using
                     gene prediction method: GeneMarkS-2+."
                     /codon_start=1
                     /transl_table=11
                     /product="hypothetical protein"
                     /protein_id="HOQ54263.1"
                     /translation="MQPRKPHIRRDRKPAQAAGDRMPVHSDPAPVQHATTGDGTLHSP
                     RNGRRERGEQTLPPAADLQHAAAVLLADVVDAGTARLEIRDPSRPNIATGATSAKSLW
                     LVESPAAASMGAHPLVYGRHRHRRRQAAELDPVHARR"
     gene            2448..2879
                     /locus_tag="PLF56_11715"
     CDS             2448..2879
                     /locus_tag="PLF56_11715"
                     /inference="COORDINATES: protein motif:HMM:NF012798.2"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_function: GO:0003677 - DNA binding [Evidence IEA];
                     GO_process: GO:0006310 - DNA recombination [Evidence IEA];
                     GO_process: GO:0015074 - DNA integration [Evidence IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="tyrosine-type recombinase/integrase"
                     /protein_id="HOQ54264.1"
                     /translation="MDRHAVTRSLRRLAVAADVRLPGLHPHMLRHTLVTTMLDAGVDL
                     RDVQIAVLRADPRATERYDRARKNLIGTRITRSPRPWRWEPERGRGCRRACGRVVPLR
                     SAPLTSAVLALDVDVQRLREVCERYGVVRLDVFGSLANGTG"
BASE COUNT          461 a         1022 c         1029 g          482 t
ORIGIN      
        1 cgctgggccg agatcgtgcc ggtgtcggcg ctgaccaacg accaggtcgg cctggtcgcc
       61 gacgtgctga tcgcccagtt gccggaaggt cccgcctact accccgaggg cgaggtgacc
      121 gacgaaccca ccgaaaccct gatcgccgag ctgatccgcg aggccgcgct cgagaaggtc
      181 cgcgacgagc tgccgcactc gatcacggtc accatcgacg agatggggtt gcgcgagggg
      241 cgtcccgagg atcgtccact gctcgacgtg tacgcctcgc tggtggtcga gcgcgactcg
      301 cagaagggca tcgtgatcgg tcatcagggc tcggtgctgc gcgagatcgg caccgcagcc
      361 cgcacccagc tgaccgcgct gctggggacg cccgtccacc tcgacctgaa ggtgaaggtg
      421 ctcaaggact ggcagcgcaa cgccaagtac ctcaaccggt tgggtttctg agttcgcctg
      481 atctatggca gacgtctctc aaccgtgttg ggaaccgtct cctgaacagg tgctggggac
      541 gggtctatct gtgctgggaa ccgtctcctc aacactgctg gggacgggtc gatccgtgct
      601 gggaaccgtc tcctgaacag tgctggggac gggtctatcc gtgctgggaa ccgtcccaag
      661 aaccctattg agcaccgccc ccgagccgtg ctgagcaccg tccccggatc agccggttct
      721 gagatcgtcc gcatgagtac cgaacgaagg cggcgtgtcg gggtgcgagc cctcggctgg
      781 tgtcgggatt tcggaactat tcacgaccga ctcagtgggg cccgcgtggg gccgcccacc
      841 cgatcctgtc ctccgcgcac cgacacacgg gcgtaatcaa cccggcgact ggctgaaaca
      901 cacggttcgt aacgtcggca ccaacgctcg cccccgagct accccaggag tgtcatgccg
      961 accgcaatcg ccgccgatct gcgagaggac gcggctgccc cgcagggggc cgatcgctga
     1021 tgccgtccgg gggtgccttg cggctggcgg agttcaacgg cctgcccgcc accgctgccc
     1081 acgagatcgt gctgcgctgc gtgtcgatca aaccctgggc gacggcgctg gtggccaggc
     1141 gcccctaccg cgaccggcaa cggttgctcg ccacggcgcg cgaactgacc gacgcctgga
     1201 cctgggccga cgtcgaggcg gcgctggacg cctggccctt tctggacgcc cgcgccgagg
     1261 gggcgcgggt gcctgccggg gtggtggtgg ccggcgcgga agggacgctg tcggccgacc
     1321 tctccgcggt ctcggaggcc taccaggaac gattcggacg cagcctcgtg gtcggcgcgt
     1381 ccgggcgcac cacctcggac gtgttgcggc tggctgtgcg gcggctactg atggacgagg
     1441 cgggcgaccg cagcgccacc acggccgaac tgcgccagat cagcctgcgc cggctgcggc
     1501 gcatgattct ctgagcggca gcgggcaaca ccgacgggcc acagccgagg cactcacccc
     1561 tccaatccgc cggccagctc gatcagggcc agatcactac ccggcggacc gaccaggcag
     1621 acccccaacg gaccctccgg cacctgaagc aggggagcgc tgacggccgg ccgtccggtg
     1681 atgccggcga ggcaggtgag ccgcatggtt gccctgcggt gcgcgtccac ggcatgcgga
     1741 gccgcccaca atctcggcgc cgcccctgcg gtcgacggca gcacgaacac ccggtcgccc
     1801 agcaactcgt tccaggcctg ccgcagccgt tcgagttgcc ccaggcgagg tcgtcctgcg
     1861 cccccctcga tcgcggacgc cgcgacgaag gcacccggcg tgacaccgct gaaggctttg
     1921 agccgccgca cttcctgagt ccaccccaca tagagcccga tgtgtaccca ctgcttggat
     1981 agcggcggca accgcgctcg gtgcaccaga ccagggacgc acgaaggtcg gactcggtat
     2041 gaacacgcgt ggcgtcgaac ctcgccaggt aggcggccac cgcacgcttg acggcgagcg
     2101 cgtcaccgtc gagcgtgcac gggatcgagt tcagcggcct gccgacgacg atggcgatgc
     2161 cgcccgtaca cgagtgggtg tgcgcccatg ctggccgccg ccggggactc caccaaccac
     2221 aacgacttcg cgctggtcgc gccggtcgcg atgttcggcc tgcttgggtc gcggatctcc
     2281 aggcgtgcgg tgccagcatc gacgacgtcg gcgaggagca cggccgccgc gtgctgaagg
     2341 tccgcggcag gtggcaaggt ctgctcaccc cgctcccgcc ggccgttgcg cgggctgtgg
     2401 agcgtgccgt cgccggtcgt ggcgtgttga accggcgcgg ggtccgaatg gaccggcatg
     2461 cggtcacccg cagcctgcgc cggcttgcgg tcgcggcgga tgtgcggctt cctgggctgc
     2521 atccgcacat gctgcggcac actttggtca ccacgatgct ggatgccggg gtcgatctgc
     2581 gcgatgtgca gatcgctgtc cttcgcgctg atccgcgtgc caccgagcgt tacgaccggg
     2641 cgcgcaagaa cctgatcggc acccgaatta ctcgctcgcc gcgtccatgg cgttgggaac
     2701 ctgaacgagg ccggggctgc cgtcgtgcgt gcggccgcgt agtgccgctg cggtcagctc
     2761 cgctgacttc ggctgtgctc gcgttggatg ttgatgtgca gcgcttgcgg gaggtatgcg
     2821 agcgctacgg agtcgtgcgt ctcgacgtgt tcgggtccct ggcgaatggc actgggtgaa
     2881 gcgcttcgat tattccgtca ggcgttgata taccgaatct accgccagaa ttcccggcca
     2941 tgccgtttgc atcgtttcat cgagaatctg gcggtagatt cggtatatct tcac
//
LOCUS       DAOSUG010000398         2980 bp    DNA     linear   ENV 10-JUN-2024
DEFINITION  MAG TPA_asm: Micropruina sp. isolate AMR_MDS_2195
            NODE_13440_length_2980_cov_3.548718, whole genome shotgun sequence.
ACCESSION   DAOSUG010000398 DAOSUG010000000
VERSION     DAOSUG010000398.1
DBLINK      BioProject: PRJNA925477
            BioSample: SAMN32864262
            Sequence Read Archive: SRR4236673
KEYWORDS    WGS; ENV; Metagenome Assembled Genome; MAG; Third Party Data; TPA;
            TPA:assembly.
SOURCE      Micropruina sp. (wastewater metagenome)
  ORGANISM  Micropruina sp.
            Bacteria; Bacillati; Actinomycetota; Actinomycetes;
            Propionibacteriales; Nocardioidaceae; Micropruina.
REFERENCE   1  (bases 1 to 2980)
  AUTHORS   Abdulkadir,N., Saraiva,J.P., Zhang,J., Stolte,S., Gillor,O.,
            Harms,H. and Rocha,U.
  TITLE     Genome-centric analyses of 165 metagenomes show that mobile genetic
            elements are crucial for the transmission of antimicrobial
            resistance genes to pathogens in activated sludge and wastewater
  JOURNAL   Microbiol Spectr 12 (3), e0291823 (2024)
   PUBMED   38289113
REFERENCE   2  (bases 1 to 2980)
  AUTHORS   Abdulkadir,N.
  TITLE     Direct Submission
  JOURNAL   Submitted (24-MAR-2023) Environmental Microbiology, Helmholtz
            Center for Environmental Research -UFZ, Permoserstr 15, Leipzig
            04318, Germany
COMMENT     The annotation was added by the NCBI Prokaryotic Genome Annotation
            Pipeline (PGAP). Information about PGAP can be found here:
            https://www.ncbi.nlm.nih.gov/genome/annotation_prok/
            
            ##Genome-Assembly-Data-START##
            Assembly Method        :: SPAdes v. 3.11.1
            Genome Representation  :: Full
            Expected Final Version :: Yes
            Genome Coverage        :: Not Applicable
            Sequencing Technology  :: Illumina MiSeq
            ##Genome-Assembly-Data-END##
            
            ##Genome-Annotation-Data-START##
            Annotation Provider               :: NCBI
            Annotation Date                   :: 05/04/2023 04:57:10
            Annotation Pipeline               :: NCBI Prokaryotic Genome
                                                 Annotation Pipeline (PGAP)
            Annotation Method                 :: Best-placed reference protein
                                                 set; GeneMarkS-2+
            Annotation Software revision      :: 6.5
            Features Annotated                :: Gene; CDS; rRNA; tRNA; ncRNA
            Genes (total)                     :: 2,776
            CDSs (total)                      :: 2,750
            Genes (coding)                    :: 2,714
            CDSs (with protein)               :: 2,714
            Genes (RNA)                       :: 26
            tRNAs                             :: 24
            ncRNAs                            :: 2
            Pseudo Genes (total)              :: 36
            CDSs (without protein)            :: 36
            Pseudo Genes (ambiguous residues) :: 0 of 36
            Pseudo Genes (frameshifted)       :: 2 of 36
            Pseudo Genes (incomplete)         :: 32 of 36
            Pseudo Genes (internal stop)      :: 2 of 36
            ##Genome-Annotation-Data-END##
FEATURES             Location/Qualifiers
     source          1..2980
                     /organism="Micropruina sp."
                     /mol_type="genomic DNA"
                     /submitter_seqid="NODE_13440_length_2980_cov_3.548718"
                     /isolate="AMR_MDS_2195"
                     /isolation_source="Activated sludge of municipal
                     wastewater treatment plants"
                     /db_xref="taxon:2737536"
                     /environmental_sample
                     /geo_loc_name="Canada"
                     /lat_lon="49.25 N 123.1 W"
                     /collection_date="2015-06-16"
                     /metagenome_source="wastewater metagenome"
                     /note="metagenomic"
     gene            <1..2103
                     /locus_tag="PLF56_11720"
     CDS             <1..2103
                     /locus_tag="PLF56_11720"
                     /inference="COORDINATES: protein motif:HMM:NF014910.2"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_function: GO:0003824 - catalytic activity [Evidence
                     IEA];
                     GO_function: GO:0050660 - flavin adenine dinucleotide
                     binding [Evidence IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="FAD-linked oxidase C-terminal domain-containing
                     protein"
                     /protein_id="HOQ54265.1"
                     /translation="GTLGVVIRATVQLSRDPAHSCLVVLGFGSLAEAADLVPEVLTYR
                     PSACEGLDARIVDTVRARHGAHAVPALPAGEGWLFVELAGDDGAEVAERARRLAAVGP
                     AARVITDAADVVTLWRLRADGAGYAAVALDAPAHPGWEDAAVPPQRLGAYLRDFEALL
                     AAHGLHGLPYGHFGEGCVHCRIDFPLGPGSGTHEYRRFVEAAADLAAGYGGSMSGEHG
                     DGRARSELLGRMYTPQALALFGAVKRLFDPHNLLNPGVLVDPAPLDANVRLAQVAHPL
                     DAFAADVHRCTGVGRCVAAGTAGAMCPSYQVTRQEKDSTRGRARVLQELVNGTLISSP
                     RSVEVAEALDLCLACKACAVECPTGVDLARDKSRTLHRAYRGRLRPPSHYTLGWLPRW
                     LRLLTDVPGAARLANAALGMPALRDLALRMAGLDARRSVPHFRENALPGEVGGHGRRA
                     DKGRQADSYGEVGRSAGGEVAVWADSFSRGFAGGQVEAALRVLSRAGYRPRLIGQPAC
                     CGLTWITTGQLDAAAGRLRRSLDVLSPWAQRGTPIVGLEPSCLAVWRDEAGELVDDPR
                     VTAVSRSLVTLAELLGGTEGWTPPDLTGRTVVAQPHCHHHAVLGWQADRALLQGTGAD
                     VVTVTGCCGLAGNFGFERGHYETSIGVAGLQLLPALDRAPHDAIVLADGFSCRTQVAD
                     LTGRRAVSLAELLDGTGD"
     gene            2365..>2980
                     /locus_tag="PLF56_11725"
     CDS             2365..>2980
                     /locus_tag="PLF56_11725"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_002516980.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_function: GO:0005524 - ATP binding [Evidence IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="ATP-binding cassette domain-containing protein"
                     /protein_id="HOQ54266.1"
                     /translation="MPEFVFTMHNVRKKLGEKLVLDNVTMSFFEGAKIGVVGPNGAGK
                     STMLKLMAGMLQPDNGDVGLKKGASVGILLQEPPLTEGRTVLENIEEAVAETKGILDR
                     YNEIGLEMAEPDADYDALLAEMDKLQTEIEHRNAWDLESQLEQAMDALQCPPGDTIVD
                     VLSGGERRRVALCKLLLQQPDLLLLDEPTNHLDAESVNWLEGHLK"
BASE COUNT          413 a         1034 c         1086 g          447 t
ORIGIN      
        1 ggcactttgg gcgtggtcat ccgcgcgacg gtgcaactga gccgcgatcc ggcgcacagc
       61 tgcctggtgg tgctcggttt cgggtcactc gccgaggccg ccgacctggt tcccgaggtg
      121 ctgacgtacc gccccagcgc ctgcgagggc ctggacgccc gcatcgtcga caccgtgcgc
      181 gcacggcacg gcgcccacgc ggttccggct ctgcctgcgg gcgaagggtg gctgttcgtc
      241 gagttggccg gcgacgacgg ggctgaggta gccgaacggg cgcggcggct ggcggccgtg
      301 ggtccggccg cccgggtgat cacggacgcg gctgacgtgg tgaccctgtg gcgactgcgt
      361 gccgacggcg ccgggtatgc cgccgtcgcc ctggacgccc cggcccaccc cggctgggag
      421 gacgccgcgg tgccgccgca gcggctgggc gcctacctgc gcgacttcga ggccctgctg
      481 gcggcgcacg gactgcacgg actgccgtac gggcatttcg gcgagggctg cgtgcattgc
      541 cggatcgact tccccctggg gcccgggagc ggcacgcacg agtatcgacg cttcgtcgag
      601 gcggcggccg acctggcggc aggctacggc gggtcgatgt cgggtgagca cggcgacggc
      661 cgggcgcgct cggagttgct gggccgcatg tacacgccgc aggctctggc cctgttcggc
      721 gcggtgaagc gcctgttcga cccgcacaac ctgctgaacc ccggcgtgct ggtcgacccc
      781 gcgccgctcg atgcgaacgt gcggctggcc caggtggccc atccgctgga cgccttcgcc
      841 gccgacgtgc accgctgcac cggcgtaggc cgatgcgtgg ccgccggtac tgccggtgcc
      901 atgtgtccct cgtaccaggt cacccggcag gagaaggatt cgacccgcgg gcgggcccgg
      961 gtgctgcagg agctggtcaa cggcaccctg atcagctcgc cccgctcggt cgaggtggcc
     1021 gaagcccttg acctgtgcct ggcctgcaag gcgtgtgccg tcgaatgccc gaccggggtg
     1081 gatctggccc gcgacaagtc gcgcacgttg catcgcgcct accgggggcg gctgcggccg
     1141 ccgagccact acacgttggg ctggctgccg cgctggttgc ggctgctcac cgacgtgcca
     1201 ggggcggccc ggttggcgaa cgccgccctc ggcatgcccg cgttgcgtga cctggccctc
     1261 cgcatggcag gcctggacgc acgccgctcg gtgccacact ttcgtgagaa cgcgctgccg
     1321 ggtgaggtgg gtggccatgg tcgacgggcc gacaagggcc ggcaagccga ttcttacggc
     1381 gaggtgggtc ggtccgcggg cggcgaggtg gccgtgtggg ccgattcgtt ctcgcgcggt
     1441 tttgccggcg gccaggtcga ggcggcgctg cgggtgctga gccgtgccgg gtaccgaccg
     1501 aggctgatcg ggcaacccgc atgctgtggt ctgacctgga tcaccaccgg tcaactggac
     1561 gccgccgccg gccggttgcg tcgctcgctg gacgtgctga gcccctgggc acagcgcggc
     1621 accccgatcg tgggcctcga accatcgtgc ctcgcggtct ggcgcgacga ggccggcgaa
     1681 ctggtggacg acccccgcgt gaccgccgtg tcgcgctcgt tggtcaccct ggcggagctg
     1741 ctgggcggca ccgagggctg gacgccgccc gacctgaccg gacgcaccgt ggtggcccag
     1801 ccgcactgcc accaccacgc cgtgctcggg tggcaggccg accgcgcgct gctgcagggc
     1861 accggggcgg atgtggtgac cgtgaccggg tgctgtggac tggcgggcaa cttcgggttc
     1921 gagcgtggcc actacgagac ctcgatcggc gtggccggcc tgcagctgtt gccggcactc
     1981 gaccgggcac cgcacgatgc gatcgtgctc gccgacggat tctcgtgccg cacccaggtg
     2041 gccgacctga ccggacgacg agcggtcagc ctcgccgagt tgctggacgg taccggggat
     2101 tgagcctgcc ggcccgcccg ggctgcgcac caacgagctg cgcaccgacg acgagcctgc
     2161 tgaacagcca gctcctggcc gcggtgccct caaggcgggc gcccctgcgc tcgaagcagc
     2221 ccagttcgag gcactctgcg ttgccctggc atcgatcgag cggccggtgc ccccgaccgg
     2281 cgccgtctcg acgatttcga cccgcgcgcg actcgatata ctcagctcgt ttgtttccgc
     2341 tcccccaccc ggtgaggttc ccttatgccc gagttcgtgt tcaccatgca caacgtccgc
     2401 aagaagctgg gcgagaagct ggtgctcgac aacgtcacca tgtcgttctt cgagggcgcc
     2461 aagatcggcg tggtcggccc caacggtgcc ggcaagtcga ccatgttgaa gttgatggcc
     2521 ggcatgctgc aacccgacaa cggcgacgtc ggcctcaaga agggggccag cgtcggcatt
     2581 ctgctgcagg agccgccgct gaccgagggc cgcacggtgc tcgagaacat cgaagaggcg
     2641 gtggccgaga ccaagggcat tctcgaccgg tacaacgaga tcggcctcga gatggccgaa
     2701 ccggacgccg actacgacgc gctcctggcc gagatggaca agctgcagac cgagatcgag
     2761 caccgcaacg catgggatct cgaatcgcag ctcgaacagg ccatggacgc cctgcaatgc
     2821 ccccctggtg acaccatcgt cgacgtactg tcgggtggcg agcgccggcg ggtggccctg
     2881 tgcaagctgc tgctgcaaca gcccgacctg ctgctgctcg acgagccgac caaccacctg
     2941 gacgccgaga gcgtgaactg gctggagggc cacctgaaga
//
LOCUS       DAOSUG010000399         2968 bp    DNA     linear   ENV 10-JUN-2024
DEFINITION  MAG TPA_asm: Micropruina sp. isolate AMR_MDS_2195
            NODE_13505_length_2968_cov_3.467216, whole genome shotgun sequence.
ACCESSION   DAOSUG010000399 DAOSUG010000000
VERSION     DAOSUG010000399.1
DBLINK      BioProject: PRJNA925477
            BioSample: SAMN32864262
            Sequence Read Archive: SRR4236673
KEYWORDS    WGS; ENV; Metagenome Assembled Genome; MAG; Third Party Data; TPA;
            TPA:assembly.
SOURCE      Micropruina sp. (wastewater metagenome)
  ORGANISM  Micropruina sp.
            Bacteria; Bacillati; Actinomycetota; Actinomycetes;
            Propionibacteriales; Nocardioidaceae; Micropruina.
REFERENCE   1  (bases 1 to 2968)
  AUTHORS   Abdulkadir,N., Saraiva,J.P., Zhang,J., Stolte,S., Gillor,O.,
            Harms,H. and Rocha,U.
  TITLE     Genome-centric analyses of 165 metagenomes show that mobile genetic
            elements are crucial for the transmission of antimicrobial
            resistance genes to pathogens in activated sludge and wastewater
  JOURNAL   Microbiol Spectr 12 (3), e0291823 (2024)
   PUBMED   38289113
REFERENCE   2  (bases 1 to 2968)
  AUTHORS   Abdulkadir,N.
  TITLE     Direct Submission
  JOURNAL   Submitted (24-MAR-2023) Environmental Microbiology, Helmholtz
            Center for Environmental Research -UFZ, Permoserstr 15, Leipzig
            04318, Germany
COMMENT     The annotation was added by the NCBI Prokaryotic Genome Annotation
            Pipeline (PGAP). Information about PGAP can be found here:
            https://www.ncbi.nlm.nih.gov/genome/annotation_prok/
            
            ##Genome-Assembly-Data-START##
            Assembly Method        :: SPAdes v. 3.11.1
            Genome Representation  :: Full
            Expected Final Version :: Yes
            Genome Coverage        :: Not Applicable
            Sequencing Technology  :: Illumina MiSeq
            ##Genome-Assembly-Data-END##
            
            ##Genome-Annotation-Data-START##
            Annotation Provider               :: NCBI
            Annotation Date                   :: 05/04/2023 04:57:10
            Annotation Pipeline               :: NCBI Prokaryotic Genome
                                                 Annotation Pipeline (PGAP)
            Annotation Method                 :: Best-placed reference protein
                                                 set; GeneMarkS-2+
            Annotation Software revision      :: 6.5
            Features Annotated                :: Gene; CDS; rRNA; tRNA; ncRNA
            Genes (total)                     :: 2,776
            CDSs (total)                      :: 2,750
            Genes (coding)                    :: 2,714
            CDSs (with protein)               :: 2,714
            Genes (RNA)                       :: 26
            tRNAs                             :: 24
            ncRNAs                            :: 2
            Pseudo Genes (total)              :: 36
            CDSs (without protein)            :: 36
            Pseudo Genes (ambiguous residues) :: 0 of 36
            Pseudo Genes (frameshifted)       :: 2 of 36
            Pseudo Genes (incomplete)         :: 32 of 36
            Pseudo Genes (internal stop)      :: 2 of 36
            ##Genome-Annotation-Data-END##
FEATURES             Location/Qualifiers
     source          1..2968
                     /organism="Micropruina sp."
                     /mol_type="genomic DNA"
                     /submitter_seqid="NODE_13505_length_2968_cov_3.467216"
                     /isolate="AMR_MDS_2195"
                     /isolation_source="Activated sludge of municipal
                     wastewater treatment plants"
                     /db_xref="taxon:2737536"
                     /environmental_sample
                     /geo_loc_name="Canada"
                     /lat_lon="49.25 N 123.1 W"
                     /collection_date="2015-06-16"
                     /metagenome_source="wastewater metagenome"
                     /note="metagenomic"
     gene            complement(22..261)
                     /locus_tag="PLF56_11730"
     CDS             complement(22..261)
                     /locus_tag="PLF56_11730"
                     /inference="COORDINATES: protein motif:HMM:NF014470.2"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_process: GO:0015031 - protein transport [Evidence IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="twin-arginine translocase TatA/TatE family
                     subunit"
                     /protein_id="HOQ54267.1"
                     /translation="MEWVILAIIALLVFGGSRLANAGKNAGKAIREFKEETSSLSASN
                     PPAVPPPATPRAAEPEGVVHEGEIVTDPKDKGQQA"
     gene            complement(321..500)
                     /locus_tag="PLF56_11735"
     CDS             complement(321..500)
                     /locus_tag="PLF56_11735"
                     /inference="COORDINATES: ab initio
                     prediction:GeneMarkS-2+"
                     /note="Derived by automated computational analysis using
                     gene prediction method: GeneMarkS-2+."
                     /codon_start=1
                     /transl_table=11
                     /product="hypothetical protein"
                     /protein_id="HOQ54268.1"
                     /translation="MMWVLIFAGIAVAGLITVVAYAIWLAHKASDLFSELQMLAKRAE
                     ELADLVSQVQLPSTE"
     gene            complement(497..1486)
                     /locus_tag="PLF56_11740"
     CDS             complement(497..1486)
                     /locus_tag="PLF56_11740"
                     /inference="COORDINATES: protein motif:HMM:NF024676.2"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="WYL domain-containing protein"
                     /protein_id="HOQ54269.1"
                     /translation="MSTSAEQVSRLLALVPYLQAHPDADLAETAAAFGVTPRRLVADL
                     EVLWYVGLPGGMPGDLIEIDMDAVESQGRISLSNAEYLSRPMRFTIDEVTSLIVALRA
                     VREVTGGAAGQAVDSALAKLATVAGAQESRRVGFAVATSESAIREQLVEAIAGARAVR
                     LTYDGLTRAETTRPIVEPHRLSVRDGYVYLEAWSRERNAWRTFRLDRIAEVGPADGGI
                     ADHGEPPPFDAGWLEVRAEAALVVLTVTDQAHWISEYYPVRSIEPVSGGWRVQLLVAD
                     PAWLRTLLLRLGPSVLAVDPPEAAESARAAASEALALYQALPASDVESVSLGP"
     gene            complement(1483..2433)
                     /locus_tag="PLF56_11745"
     CDS             complement(1483..2433)
                     /locus_tag="PLF56_11745"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_013161239.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="WYL domain-containing protein"
                     /protein_id="HOQ54270.1"
                     /translation="MAPRKSERIMNLAICLLLARRFLDKNHIRDVVEGYAGLSDAAFE
                     RTFERDKDDLRAMGVPVETGSNSSLFPDELGYRIRRQDFELPPIDFTAAEATALGLAS
                     QVWGTAALAEHTVSALAKLRAAGVDPDASRLTGLSPSIGAKEPAFEPLWQATISRTAV
                     AFGYRGVARDVEPWILTYRRGSWYLAGRDRTKQAPRMFKLARIDDPPHAIGKPGAYEV
                     PDVDLAELTRSLEPAPADAYAVLAIRGDRAPDLRRRGQRVDDPPHLPPGFTAWRVPFV
                     QDRDFVAEVCGHGPAVLVWEPLELRRQVVARLTAIAEAGR"
     gene            2624..>2968
                     /locus_tag="PLF56_11750"
     CDS             2624..>2968
                     /locus_tag="PLF56_11750"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_002548728.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_function: GO:0004812 - aminoacyl-tRNA ligase activity
                     [Evidence IEA];
                     GO_function: GO:0005524 - ATP binding [Evidence IEA];
                     GO_process: GO:0043039 - tRNA aminoacylation [Evidence
                     IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="glutamate--tRNA ligase family protein"
                     /protein_id="HOQ54271.1"
                     /translation="MAGRFAPSPTSALHLGNLRTALVAWLAARSTGRSFLLRIEDLDT
                     QRVAAAPYVAAGQLADLAALGLDFDGEVVWQSQRGARYAEAVAGLPTYECFCTRREIA
                     EAASAPHGDGYRP"
BASE COUNT          446 a         1023 c         1060 g          439 t
ORIGIN      
        1 gaggcgtcga gccaccggag gtcaggcctg ctggcccttg tccttcgggt cggtgacgat
       61 ctcaccctca tgcacgacgc cctcgggttc agcggcccgc ggcgtggccg gcggcggcac
      121 cgccggcggg ttggaggcac tcaacgacga ggtctcttct ttgaactcac ggatcgcctt
      181 gccggcattc ttgccggcgt tggcgaggcg actaccgccg aaaaccagaa gggcgatgat
      241 ggccaggatg acccattcca taccgccggg caggccgaac atagtggttg cgctccttgg
      301 gttgtgacaa cttcgtcagt ctactcagtg ctcggcagct gcacctgtga cacgaggtcg
      361 gcgagttctt cggcccgttt cgcgagcatt tgcagctcgc tgaacagatc ggacgccttg
      421 tgcgccagcc agatcgcata cgcaacgacg gtgatcaggc cggccacggc aatgcccgcg
      481 aagatcagca cccacatcat gggccgagac taaccgattc gacgtcggat gcgggcagtg
      541 cctggtagag cgcgagggct tccgacgccg ccgcccgggc cgactcggcc gcctcgggcg
      601 ggtcgacagc caacacagac gggccgagcc gtagcagcag cgtccgaagc caggcggggt
      661 cagcgaccag caactgcacc cgccagccgc cgctcaccgg ctcgatggag cgcaccgggt
      721 agtactcgct gatccagtgc gcctggtcgg tgacggtgag caccaccaac gcggcctcgg
      781 cccgcacctc gagccagccc gcatcgaagg gcggtggctc gccatggtcg gcaatgccgc
      841 cgtccgccgg cccgacctcg gcgatgcggt ccagccggaa ggtgcgccag gcgttgcgct
      901 cgcgactcca cgcctcgagg tacacatacc cgtcgcggac gctgagccgg tgcggttcga
      961 cgatcggacg ggtggtctcg gcccgggtca agccgtcata ggtgagccgg acggcccggg
     1021 ccccggcgat cgcctcgacc aactgctcgc ggatcgcgct ctcggaggtg gccaccgcga
     1081 acccgacccg ccggctctct tgcgcgcccg ccaccgtggc gagcttcgcc agtgcggaat
     1141 caacagcctg tccggcggcg ccgccggtca cctcgcggac ggcccgcagc gcgacgatca
     1201 ggctagtcac ctcatcgatg gtgaaccgca tcggacggga caggtactcg gcattgctca
     1261 ggctgatccg gccctgggac tcgaccgcgt ccatgtcgat ctcgatcagg tcgccgggca
     1321 taccgccggg caggccgacg taccagagca cctccaggtc ggcgaccagc cggcgcggtg
     1381 tcacgccgaa ggcggcggcc gtctcggcga ggtcggcgtc cgggtgggcc tgcaggtacg
     1441 gcaccagggc cagcagccgc gacacctgct cggcggaggt gctcatcggc cggcctcggc
     1501 gatggcggtc agccgggcga ccacctgacg gcgcaactcg aggggctccc acaccagcac
     1561 cgccggcccg tggccgcaca cctcggcgac gaaatcgcga tcctgcacga acggcacccg
     1621 ccaggccgtg aagccgggcg gcaggtgcgg cgggtcgtcc acccgctgcc cacggcggcg
     1681 caggtcgggc gcgcggtcgc cgcggatggc gagcacggcg tacgcgtcgg ccggtgccgg
     1741 ttcgagcgag cgggtcagtt cggccagatc gacgtcgggc acctcgtagg caccgggctt
     1801 gccgatggcg tggggcgggt cgtcgatgcg ggccagcttg aacatgcggg gggcctgctt
     1861 ggtgcggtcg cgtccggcca ggtaccacga gccgcgccga taggtgagaa tccagggctc
     1921 gacgtcgcga gccacgccgc ggtacccgaa cgcgaccgcg gtgcggctga tggtggcctg
     1981 ccagagcggt tcgaacgcgg gttccttggc gccgatgctg gggctcagac cggtcagccg
     2041 ggacgcgtcg gggtcgacgc cggccgcgcg aagcttggcc agcgcgctga ccgtgtgttc
     2101 ggccagcgcg gcggtgcccc acacctggct ggccaggccg agcgcggtgg cttcggcggc
     2161 ggtgaagtcg atcgggggca actcgaaatc ctggcggcgg atgcggtagc cgagctcgtc
     2221 cggaaaaagg gaggagttac tgccggtctc gaccggcacc cccatcgccc gcaggtcgtc
     2281 cttgtcgcgc tcgaacgtgc gctcgaaggc cgcatcagac aggcccgcat acccctcgac
     2341 cacgtcacgg atgtggttct tgtcgagaaa ccgccgggcc agcagcaggc agatcgcgag
     2401 gttcatgatg cgctcggatt tgcgtggcgc catcgttgct cctgcctgcc ggctgccttg
     2461 aggctgaacg tatcactggg gcggccgtga ttctgaaagg tcgacaccag tcgcacgctg
     2521 gggtggcgac acgccggtga actctgtgac ccgtgcgggt gatttcagaa tcgtgggtgg
     2581 gcttcgcctc gccgggcccc gggtgttgcg caagaatcgg cacatggccg gccggttcgc
     2641 accctcgccc acctcggcgt tgcatctggg caacctgcgc acagccctgg tggcctggct
     2701 ggccgcccgc agcaccggcc gaagctttct gctgcgcatc gaggatctcg acacccagcg
     2761 ggtggcggcg gcgccctatg tggccgcggg ccaactggct gacctggcgg ccctcgggct
     2821 cgacttcgac ggcgaagtgg tgtggcagtc gcagcgcggt gcgcgctacg ccgaggcggt
     2881 ggccggcctg ccgacctatg agtgcttctg cacgcggcgc gagatcgccg aagcggcatc
     2941 cgcaccgcat ggagacgggt atcgcccc
//
LOCUS       DAOSUG010000400         2950 bp    DNA     linear   ENV 10-JUN-2024
DEFINITION  MAG TPA_asm: Micropruina sp. isolate AMR_MDS_2195
            NODE_13584_length_2950_cov_3.908463, whole genome shotgun sequence.
ACCESSION   DAOSUG010000400 DAOSUG010000000
VERSION     DAOSUG010000400.1
DBLINK      BioProject: PRJNA925477
            BioSample: SAMN32864262
            Sequence Read Archive: SRR4236673
KEYWORDS    WGS; ENV; Metagenome Assembled Genome; MAG; Third Party Data; TPA;
            TPA:assembly.
SOURCE      Micropruina sp. (wastewater metagenome)
  ORGANISM  Micropruina sp.
            Bacteria; Bacillati; Actinomycetota; Actinomycetes;
            Propionibacteriales; Nocardioidaceae; Micropruina.
REFERENCE   1  (bases 1 to 2950)
  AUTHORS   Abdulkadir,N., Saraiva,J.P., Zhang,J., Stolte,S., Gillor,O.,
            Harms,H. and Rocha,U.
  TITLE     Genome-centric analyses of 165 metagenomes show that mobile genetic
            elements are crucial for the transmission of antimicrobial
            resistance genes to pathogens in activated sludge and wastewater
  JOURNAL   Microbiol Spectr 12 (3), e0291823 (2024)
   PUBMED   38289113
REFERENCE   2  (bases 1 to 2950)
  AUTHORS   Abdulkadir,N.
  TITLE     Direct Submission
  JOURNAL   Submitted (24-MAR-2023) Environmental Microbiology, Helmholtz
            Center for Environmental Research -UFZ, Permoserstr 15, Leipzig
            04318, Germany
COMMENT     The annotation was added by the NCBI Prokaryotic Genome Annotation
            Pipeline (PGAP). Information about PGAP can be found here:
            https://www.ncbi.nlm.nih.gov/genome/annotation_prok/
            
            ##Genome-Assembly-Data-START##
            Assembly Method        :: SPAdes v. 3.11.1
            Genome Representation  :: Full
            Expected Final Version :: Yes
            Genome Coverage        :: Not Applicable
            Sequencing Technology  :: Illumina MiSeq
            ##Genome-Assembly-Data-END##
            
            ##Genome-Annotation-Data-START##
            Annotation Provider               :: NCBI
            Annotation Date                   :: 05/04/2023 04:57:10
            Annotation Pipeline               :: NCBI Prokaryotic Genome
                                                 Annotation Pipeline (PGAP)
            Annotation Method                 :: Best-placed reference protein
                                                 set; GeneMarkS-2+
            Annotation Software revision      :: 6.5
            Features Annotated                :: Gene; CDS; rRNA; tRNA; ncRNA
            Genes (total)                     :: 2,776
            CDSs (total)                      :: 2,750
            Genes (coding)                    :: 2,714
            CDSs (with protein)               :: 2,714
            Genes (RNA)                       :: 26
            tRNAs                             :: 24
            ncRNAs                            :: 2
            Pseudo Genes (total)              :: 36
            CDSs (without protein)            :: 36
            Pseudo Genes (ambiguous residues) :: 0 of 36
            Pseudo Genes (frameshifted)       :: 2 of 36
            Pseudo Genes (incomplete)         :: 32 of 36
            Pseudo Genes (internal stop)      :: 2 of 36
            ##Genome-Annotation-Data-END##
FEATURES             Location/Qualifiers
     source          1..2950
                     /organism="Micropruina sp."
                     /mol_type="genomic DNA"
                     /submitter_seqid="NODE_13584_length_2950_cov_3.908463"
                     /isolate="AMR_MDS_2195"
                     /isolation_source="Activated sludge of municipal
                     wastewater treatment plants"
                     /db_xref="taxon:2737536"
                     /environmental_sample
                     /geo_loc_name="Canada"
                     /lat_lon="49.25 N 123.1 W"
                     /collection_date="2015-06-16"
                     /metagenome_source="wastewater metagenome"
                     /note="metagenomic"
     gene            complement(<1..108)
                     /locus_tag="PLF56_11755"
     CDS             complement(<1..108)
                     /locus_tag="PLF56_11755"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_005123418.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="thymidylate synthase"
                     /protein_id="HOQ54272.1"
                     /translation="MQQYLDLLRLVRDHGVEKGDRTGTGTRSIFGHQMRF"
     gene            complement(112..711)
                     /gene="rdgB"
                     /locus_tag="PLF56_11760"
     CDS             complement(112..711)
                     /gene="rdgB"
                     /locus_tag="PLF56_11760"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_014846050.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_function: GO:0016462 - pyrophosphatase activity
                     [Evidence IEA];
                     GO_process: GO:0006281 - DNA repair [Evidence IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="RdgB/HAM1 family non-canonical purine NTP
                     pyrophosphatase"
                     /protein_id="HOQ54273.1"
                     /translation="MKLVLATHNAKKLAELRRVVAEQGADLEILGLDDLPPYPEPAET
                     ERTFEGNALLKARDCRDATGQAALADDSGIEVDLLNNMPGVRSARWAGTGASDRQNLE
                     LLIAQLSDTDPAQRTGRFVAAVAFVDPDGTEHVLRATMEGHLVSSPRGEHGFGYDPIF
                     VTDGQTRTNAELTPSEKDAISHRGKAVRAMLAWLAEREE"
     gene            complement(708..1433)
                     /gene="rph"
                     /locus_tag="PLF56_11765"
     CDS             complement(708..1433)
                     /gene="rph"
                     /locus_tag="PLF56_11765"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_016669555.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_function: GO:0004549 - tRNA-specific ribonuclease
                     activity [Evidence IEA];
                     GO_process: GO:0008033 - tRNA processing [Evidence IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="ribonuclease PH"
                     /protein_id="HOQ54274.1"
                     /translation="MTYTRVDGRAADQLRPVTITRNWLDHAEGSVLVEFGRTRVLIAA
                     SVTQGVPRWRKGSGLGWVTSEYAMLPRATHSRSDRESVKGRIGGRTHEISRLIGRSLR
                     AVIDYRALGENTIVLDCDVLQADGGTRTAAITGAYVALADAVEWLRGRDALAGEPLTG
                     SVAAVSVGVVEGTPLLDLAYVEDSAAEVDLNVVTTGDARFVEVQGTAEGQPFDRAVLD
                     GLLDLGVAGTAQLTALQQQALAR"
     gene            1531..2358
                     /locus_tag="PLF56_11770"
     CDS             1531..2358
                     /locus_tag="PLF56_11770"
                     /inference="COORDINATES: ab initio
                     prediction:GeneMarkS-2+"
                     /note="Derived by automated computational analysis using
                     gene prediction method: GeneMarkS-2+."
                     /codon_start=1
                     /transl_table=11
                     /product="hypothetical protein"
                     /protein_id="HOQ54275.1"
                     /translation="MSRQDEAAAAGRSKRPSLALVGSVAFTIGATVMILLHEASHAVA
                     GLVLGHHPLQLPFAVDYRPTLGPAAEGVTAITGPLFSLVSGMIGVVIDRTVRPLRSRP
                     FARLVWLWLVFTSLQEGFGYFTIAALGDVGDTAVAFHAWGAPDWAFVAAAVFGVGGQV
                     LAAWLFSEPLRRMCRSVDDRRDLTVWAWLLGTGAMLALMTLYVLAAPAFRLDAKVTVL
                     AGAVSLGVFAPMSMMFGPRRNTAADRLPPARPPLAGWIALASLVTFNLSLLLGWLWP"
     gene            complement(2321..2812)
                     /locus_tag="PLF56_11775"
     CDS             complement(2321..2812)
                     /locus_tag="PLF56_11775"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_008359973.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="OsmC family protein"
                     /protein_id="HOQ54276.1"
                     /translation="MSDEHHYRLTTTWTGNRGTGTSGYRDYERSVSVEVSGKPVVIGS
                     ADKPFRGDPQRWNPEDLLVAALSQCHLLSYLHACVVHGVVVTHYRDEASGTLVQRGNG
                     GAFRDVLLRPQVVVADASMIEAATSAHHDAHEWCFIANSVNFPVRVEPTIRARANPAA
                     TKG"
     gene            2857..>2950
                     /locus_tag="PLF56_11780"
     CDS             2857..>2950
                     /locus_tag="PLF56_11780"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_002514674.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="glutamate racemase"
                     /protein_id="HOQ54277.1"
                     /translation="MSDTGGPIGIFDSGFGGLTVARSVVDLMPYE"
BASE COUNT          400 a         1032 c         1021 g          497 t
ORIGIN      
        1 gaagcgcatc tggtggccga agatgctgcg ggtgccggtg ccggtgcgat cacccttctc
       61 gacgccgtgg tcgcggacca gccgcaacag gtccaggtac tgctgcactt ctcattcctc
      121 ccgctcggcc aaccaggcca gcatggcgcg gacggccttg ccgcggtgac tgatcgcgtc
      181 cttttcggac ggcgtcagct cggcattcgt tcgggtttgg ccgtccgtca cgaaaatcgg
      241 atcgtacccg aagccgtgct ccccccgggg gctcgacacg aggtggccct ccatggtggc
      301 gcgcagcacg tgctcggtgc cgtcggggtc gacgaaggcg acggcggcca cgaaccgtcc
      361 ggtgcgctgc gccgggtcgg tgtcggagag ttgtgcgatc agaagctcga ggttctgccg
      421 gtcggacgcc cccgtgccgg cccagcgcgc cgaacggacg ccgggcatgt tgttgagcag
      481 gtcgacttcg atgccggaat cgtcggccag cgcggcctgg ccggtggcgt cgcggcagtc
      541 gcgggccttg agcagggcgt tgccctcgaa cgtgcgctcg gtctcagcgg gttcggggta
      601 gggcggcagg tcgtccaggc cgagaatctc gagatcggcg ccttgttcgg cgaccacccg
      661 gcgcagctcg gcgagcttct tggcgttgtg cgttgcgaga acgagcttca ccgggcgagc
      721 gcctgctgct gaagcgctgt gagttgcgcc gtgccggcca ccccgagatc gagcaggccg
      781 tcgagcaccg cccggtcgaa gggctgcccc tccgcggtgc cctgcacctc gacgaagcgc
      841 gcgtcgccgg tggtgaccac gttgagatcg acctcagcgg ccgaatcctc cacgtaggcc
      901 aggtcgagca gcggggtgcc ctcgacgacg ccgaccgaga cggcggccac cgagccggtc
      961 agcggctcgc cggccaacgc gtcacgaccg cgcagccatt cgacggcatc ggccagggcc
     1021 acgtaggcac cggtgatcgc ggcggtgcgg gtacctccgt cggcctgcag aacgtcgcag
     1081 tcgagcacga tcgtgttctc gcccagggcc cggtagtcga tcaccgcgcg caaggagcgt
     1141 ccgatcaacc gggagatctc gtgggtgcgt ccgccgatcc ggcccttcac cgactcgcgg
     1201 tcgctacggc tgtgcgtggc ccgcggcagc atcgcgtact cgctggtcac ccaccccagc
     1261 cccgagccct tgcgccaccg cgggacgccc tgggtgacgc tggcggcgat cagcacgcgg
     1321 gtgcgtccga actcgacgag cacggagccc tcggcgtggt cgagccagtt acgggtgatg
     1381 gtgacgggac gaagctggtc ggcggcccgg ccgtcgacgc gggtgtaggt catgggtcca
     1441 tcccacgacc tcctcccccg ggctgtcaaa acccggccct cacctggccc accggtgtcg
     1501 cgaagctcac gatgtgccac tcttttcagc atgagtcgtc aggacgaggc tgcggccgcc
     1561 ggtcgatcga aacgcccgtc gttggccctg gtcggctcgg tggccttcac gatcggtgcc
     1621 accgtgatga tcctgctgca cgaggccagt catgccgtcg ccggactggt actgggccac
     1681 caccccctgc agcttccgtt cgccgtcgac taccggccga cgctcggccc agcagccgag
     1741 ggcgtcacgg ccatcaccgg tccgctgttc agcctggtca gcggcatgat cggggtggtc
     1801 atcgaccgca ccgtgcgccc attgcggtcg aggccgttcg ccagattggt gtggctgtgg
     1861 ctggtgttca ccagcctgca ggagggcttc ggctacttca ccatcgcggc cctcggcgac
     1921 gtcggcgaca cggcggtcgc gtttcacgcc tggggtgcgc ccgactgggc gttcgtcgct
     1981 gctgccgtgt tcggcgtggg cggccaggtg ctggccgcgt ggctgttcag cgagccgctg
     2041 cgcagaatgt gccgatcggt cgacgaccgc cgcgacctca ccgtatgggc ctggctgctc
     2101 ggcaccgggg cgatgctggc cctgatgact ctgtacgtgc tcgccgcccc cgcgttccga
     2161 ctcgatgcga aggtcaccgt gctggccggc gcggtgtccc tcggggtgtt cgcgccgatg
     2221 tcgatgatgt tcggcccacg gcgcaacacg gccgccgacc ggctaccgcc cgctcgtcca
     2281 ccactggccg gctggatcgc gctggcgtcg ctggtgacct tcaacctttc gttgctgctg
     2341 ggttggctct ggccctgatc gtgggctcga cgcgcaccgg aaagttcacc gagttcgcga
     2401 tgaagcacca ctcgtgcgcg tcgtgatggg cgctggtggc cgcctcgatc attgaagcgt
     2461 cggccaccac cacctgcgga cgcagcagca cgtcgcggaa agcgccgccg ttgccccgct
     2521 gcaccagggt gcccgacgcc tcgtcgcggt agtgcgtgac caccacgccg tgcaccacgc
     2581 aggcgtgcag gtaggacagc aggtggcact gcgacaacgc cgccaccagc agatcctccg
     2641 gattccaccg ctgcggatcg ccccggaacg gcttgtcggc cgagccaatc accaccggct
     2701 tgccgctcac ctcgacgctc accgaccgct cgtagtcgcg atacccggac gttcccgtgc
     2761 cccggttgcc cgtccaggtg gtggtcagcc ggtaatggtg ttcgtcgctc atgccgcaca
     2821 gcgtcgcaca ccgggcagac agtaggctca ggcttcgtga gcgacaccgg gggcccgatc
     2881 ggcatcttcg attcggggtt cggggggctg accgtcgccc gctcggtggt cgacctgatg
     2941 ccttatgagg
//
LOCUS       DAOSUG010000401         2949 bp    DNA     linear   ENV 10-JUN-2024
DEFINITION  MAG TPA_asm: Micropruina sp. isolate AMR_MDS_2195
            NODE_13589_length_2949_cov_3.412923, whole genome shotgun sequence.
ACCESSION   DAOSUG010000401 DAOSUG010000000
VERSION     DAOSUG010000401.1
DBLINK      BioProject: PRJNA925477
            BioSample: SAMN32864262
            Sequence Read Archive: SRR4236673
KEYWORDS    WGS; ENV; Metagenome Assembled Genome; MAG; Third Party Data; TPA;
            TPA:assembly.
SOURCE      Micropruina sp. (wastewater metagenome)
  ORGANISM  Micropruina sp.
            Bacteria; Bacillati; Actinomycetota; Actinomycetes;
            Propionibacteriales; Nocardioidaceae; Micropruina.
REFERENCE   1  (bases 1 to 2949)
  AUTHORS   Abdulkadir,N., Saraiva,J.P., Zhang,J., Stolte,S., Gillor,O.,
            Harms,H. and Rocha,U.
  TITLE     Genome-centric analyses of 165 metagenomes show that mobile genetic
            elements are crucial for the transmission of antimicrobial
            resistance genes to pathogens in activated sludge and wastewater
  JOURNAL   Microbiol Spectr 12 (3), e0291823 (2024)
   PUBMED   38289113
REFERENCE   2  (bases 1 to 2949)
  AUTHORS   Abdulkadir,N.
  TITLE     Direct Submission
  JOURNAL   Submitted (24-MAR-2023) Environmental Microbiology, Helmholtz
            Center for Environmental Research -UFZ, Permoserstr 15, Leipzig
            04318, Germany
COMMENT     The annotation was added by the NCBI Prokaryotic Genome Annotation
            Pipeline (PGAP). Information about PGAP can be found here:
            https://www.ncbi.nlm.nih.gov/genome/annotation_prok/
            
            ##Genome-Assembly-Data-START##
            Assembly Method        :: SPAdes v. 3.11.1
            Genome Representation  :: Full
            Expected Final Version :: Yes
            Genome Coverage        :: Not Applicable
            Sequencing Technology  :: Illumina MiSeq
            ##Genome-Assembly-Data-END##
            
            ##Genome-Annotation-Data-START##
            Annotation Provider               :: NCBI
            Annotation Date                   :: 05/04/2023 04:57:10
            Annotation Pipeline               :: NCBI Prokaryotic Genome
                                                 Annotation Pipeline (PGAP)
            Annotation Method                 :: Best-placed reference protein
                                                 set; GeneMarkS-2+
            Annotation Software revision      :: 6.5
            Features Annotated                :: Gene; CDS; rRNA; tRNA; ncRNA
            Genes (total)                     :: 2,776
            CDSs (total)                      :: 2,750
            Genes (coding)                    :: 2,714
            CDSs (with protein)               :: 2,714
            Genes (RNA)                       :: 26
            tRNAs                             :: 24
            ncRNAs                            :: 2
            Pseudo Genes (total)              :: 36
            CDSs (without protein)            :: 36
            Pseudo Genes (ambiguous residues) :: 0 of 36
            Pseudo Genes (frameshifted)       :: 2 of 36
            Pseudo Genes (incomplete)         :: 32 of 36
            Pseudo Genes (internal stop)      :: 2 of 36
            ##Genome-Annotation-Data-END##
FEATURES             Location/Qualifiers
     source          1..2949
                     /organism="Micropruina sp."
                     /mol_type="genomic DNA"
                     /submitter_seqid="NODE_13589_length_2949_cov_3.412923"
                     /isolate="AMR_MDS_2195"
                     /isolation_source="Activated sludge of municipal
                     wastewater treatment plants"
                     /db_xref="taxon:2737536"
                     /environmental_sample
                     /geo_loc_name="Canada"
                     /lat_lon="49.25 N 123.1 W"
                     /collection_date="2015-06-16"
                     /metagenome_source="wastewater metagenome"
                     /note="metagenomic"
     gene            complement(<1..611)
                     /locus_tag="PLF56_11785"
     CDS             complement(<1..611)
                     /locus_tag="PLF56_11785"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_015069149.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="MFS transporter"
                     /protein_id="HOQ54278.1"
                     /translation="MPATQEKTYLKWYNKIGYGSGDVGGNVVYAFLSAFVMIYLTDAV
                     GLNPGIIGTLIMASRLFDGVSDLFFGNLIDRTHTKMGKARPWMFWAYFGCAATLIATF
                     AIPTNVGQTAQYAWFFIAYTLLNAVFFTANNIAFSALTALITKNGAERVQMGSIRFVF
                     AFGTSMLIQTFTVAGVQQFGGGAEGWRTIAIIYAVIGLAVNSLS"
     gene            complement(634..>2949)
                     /locus_tag="PLF56_11790"
     CDS             complement(634..>2949)
                     /locus_tag="PLF56_11790"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_014847725.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_function: GO:0004553 - hydrolase activity, hydrolyzing
                     O-glycosyl compounds [Evidence IEA];
                     GO_process: GO:0005975 - carbohydrate metabolic process
                     [Evidence IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="glycoside hydrolase family 2 TIM barrel-domain
                     containing protein"
                     /protein_id="HOQ54279.1"
                     /translation="ADGTRVASGELQEFSGDDTYTHRVQLRVEDPQLWTAENPYLYTL
                     TLTSPQEVVTDRVGIREITVVNAVVHVNGQPIKLRGVNRHDSDPVTGPAIDLDHMKTD
                     LRLMKQHNINAVRSAHYPNAPRFYQLCDEYGFFVMGEADNESHGTQFQFLEDPSFANQ
                     VEHWNEPIADNPEWIEATLDRTRLCVIREKNRPSIVMWSAGNEGAYGCTFEAALAWMA
                     EFDPTRLTHYESAFYRNSKRKYDYSNIDLYSRMYPALHEIHEYLDSDPDKPFILVEYS
                     HAMGNGPGDLENYWEIIRADERMCGGFVWEWCDHAVYKGKADNGKDIYFYGGDHGEDI
                     HDGNFCMDGLVYPDRTPHTGLLELKNVHRPVRVLGFDQHTGRLTLRNDMDFTDLRDHI
                     YLTCGVVCDGEVVKTVRVDLDESVPPRSETTVTLTPTVPEAGTCHLLVTSHLKGEVPF
                     LTEGHELGFDELELTTADGRNQRAVALLEGTEPGSGRLRVTEDASSITVESEAFRYAF
                     DTRTGLPQTMTLGGRDLLDAPAQINIWRAPTDNDMYIKAEWRRAHYDAASARAYSASV
                     EPGADAVTLTFPLAVVAPTIQPILKVEAAWHVNTSGAVQASLSVRRDPAFPSLPRFGI
                     RLFLPKEMSQVTYCGLGPFESYVDKRRASHHGLFGSDVFRLHEDYLRPQENGSHADCD
                     YVIVAGGGLSLTAVGSETFSFNASPYTQEELARRAHSYELVPAGSTVLCLDHANAGIG
                     SNSCGPELLEKYRVDAETFRFDITLIPTIEQ"
BASE COUNT          517 a          864 c         1003 g          565 t
ORIGIN      
        1 gacagcgagt tcacggctag cccgatcacg gcgtagatga tggcgatcgt gcgccagccc
       61 tcggcgccgc caccgaactg ttgcacacct gcgacggtga aggtctggat gagcatgctc
      121 gtcccgaagg cgaacacgaa gcggatcgag cccatctgca cacgctcggc accattcttg
      181 gtgatgagcg ccgtcaacgc cgagaaggcg atgttgttgg ccgtgaagaa gaccgcgttc
      241 agcagcgtgt aggcgatgaa gaaccaggcg tattgcgcgg tctggccgac gttggtcggg
      301 atcgcgaagg tggcgatgag cgtggcggcg caaccgaagt acgcccagaa catccacgga
      361 cgtgccttgc ccatcttcgt gtgcgtgcgg tcgatgaggt ttccgaagaa gaggtcactg
      421 accccgtcga aaagccggga ggccatgatc aatgtgccga tgatgcctgg gttcaagccc
      481 acggcatcgg tgaggtagat catgacgaac gcggacagga acgcatacac gacgttcccg
      541 ccgacatcgc ccgatccata accgatcttg ttgtaccact tgaggtacgt cttctcttgg
      601 gtggctggca cgtcgttgtg acccttcact agttcactgc tcgatggtgg gaatcagggt
      661 gatgtcgaac cggaacgtct cggcatccac gcggtacttt tcgaggagtt caggaccgca
      721 gctgttggac ccgatcccgg cgttggcgtg gtccaggcac aaaacggtgc tgcccgccgg
      781 tacgagttca tagctgtggg cacgtctcgc cagctcttcc tgcgtgtaag gggaggcgtt
      841 gaaggagaag gtctcgctcc ccacggcggt gagggacaga ccgcctccgg ccacgatgac
      901 gtagtcacag tccgcgtggc tgccgttctc ctgcggacgc aggtagtcct cgtgcaggcg
      961 gaagacgtcg gagccaaaga ggccgtgatg ggaagctcgg cgtttgtcga cgtagctctc
     1021 gaacggaccc aagccgcaat aggtaacctg cgacatctcc ttaggcagga acagcctgat
     1081 cccgaagcgg ggcagcgacg gaaaggcagg gtcacgcctc actgagaggg acgcctgcac
     1141 cgcaccggag gtgttcacgt gccaggcggc ctccaccttc aggatcggct ggatcgtggg
     1201 cgccacgacc gcgaggggga aggtgagcgt caccgcatcc gcgcctggct cgacgcttgc
     1261 ggagtaggcg cgtgccgagg cggcgtcgta gtgggcgcgt ctccactcgg ctttgatgta
     1321 catgtcgttg tccgtcggtg cccgccagat attgatctgc gcgggagcat cgagcagatc
     1381 ccgaccgccg agggtcatgg tctggggcag ccctgtgcgc gtgtcgaagg cgtaccggaa
     1441 cgcctcggac tcgacggtga tggaggaggc gtcctcggtg acgcgcaacc ggcccgagcc
     1501 cggctcggtg ccctccagga gagcgaccgc cctctggttg cgtccatcgg cggtggtcag
     1561 ttcgagttcg tcgaagccga gctcatgccc ctccgtgagg aatggcacct cgcccttcag
     1621 atgagacgtc acgagcaggt ggcaggtgcc cgcctcgggc acggtcggcg tgagcgtcac
     1681 ggttgtctcg gaccgcggcg gcacgctctc gtcgagatcg acgcgcacag tcttgaccac
     1741 ctcgccgtcg caaacgactc cgcaggtcag gtagatgtga tcgcgcagat cggtgaagtc
     1801 catgtcgttg cgcagggtga gccggccggt gtgctggtcg aacccgagga cgcggacagg
     1861 ccggtggacg ttcttgagct ccaacagacc cgtgtggggc gtgcgatcgg ggtagacgag
     1921 accatccatg cagaagttgc cgtcgtggat gtcttcgccg tggtcaccgc catagaaata
     1981 gatgtccttg ccgttgtcgg ccttgccctt gtagacggcg tggtcgcacc attcccacac
     2041 gaagccgccg cacatgcgct cgtcggcccg gatgatctcc cagtagtttt cgaggtcacc
     2101 ggggccgttg cccatggcgt ggctgtattc cacgaggatg aacggcttgt ccgggtcgga
     2161 atcgaggtac tcgtgaatct cgtgcagcgc ggggtacatc cggctgtaga ggtcgatgtt
     2221 cgagtagtcg tacttccgct tgctgttacg gtagaaggcg ctctcgtagt gcgtaaggcg
     2281 ggtggggtcg aactcggcca tccacgcgag cgcggcttcg aaagtgcacc cgtatgcgcc
     2341 ctcgttgccc gccgaccaca tgacgatgct cgggcggttc ttctcacgga tgacgcaaag
     2401 tcgggtgcgg tcgagggtcg cctcgatcca ctcggggttg tccgcgatcg gctcgttcca
     2461 gtgttcgacc tgattcgcga aggaggggtc ctccaggaac tgaaactggg tgccgtggct
     2521 ctcgttgtcg gcctcgccca tgacgaagaa tccatattcg tcgcacagct ggtaaaagcg
     2581 cggcgcgttt ggatagtgag cgctgcgcac cgcgttgatg ttgtgctgct tcatcaaccg
     2641 caggtcggtt ttcatgtgat cgaggtcgat ggccgggccg gtgaccgggt cggagtcgtg
     2701 gcggttgacg ccgcgcagct tgatcggctg cccattgacg tggacgacgg cattcacgac
     2761 cgtgatctca cggatgccga cgcggtcggt gacgacctcc tgcggtgagg tgagggtgag
     2821 ggtatagagg taaggattct cggcggtcca cagctgcggg tcctcaacgc gcagctggac
     2881 ccggtgggtg tacgtgtcgt cgccggagaa ctcctgcaac tcaccgcttg cgacgcgcgt
     2941 cccatcggc
//
LOCUS       DAOSUG010000402         2948 bp    DNA     linear   ENV 10-JUN-2024
DEFINITION  MAG TPA_asm: Micropruina sp. isolate AMR_MDS_2195
            NODE_13594_length_2948_cov_4.978223, whole genome shotgun sequence.
ACCESSION   DAOSUG010000402 DAOSUG010000000
VERSION     DAOSUG010000402.1
DBLINK      BioProject: PRJNA925477
            BioSample: SAMN32864262
            Sequence Read Archive: SRR4236673
KEYWORDS    WGS; ENV; Metagenome Assembled Genome; MAG; Third Party Data; TPA;
            TPA:assembly.
SOURCE      Micropruina sp. (wastewater metagenome)
  ORGANISM  Micropruina sp.
            Bacteria; Bacillati; Actinomycetota; Actinomycetes;
            Propionibacteriales; Nocardioidaceae; Micropruina.
REFERENCE   1  (bases 1 to 2948)
  AUTHORS   Abdulkadir,N., Saraiva,J.P., Zhang,J., Stolte,S., Gillor,O.,
            Harms,H. and Rocha,U.
  TITLE     Genome-centric analyses of 165 metagenomes show that mobile genetic
            elements are crucial for the transmission of antimicrobial
            resistance genes to pathogens in activated sludge and wastewater
  JOURNAL   Microbiol Spectr 12 (3), e0291823 (2024)
   PUBMED   38289113
REFERENCE   2  (bases 1 to 2948)
  AUTHORS   Abdulkadir,N.
  TITLE     Direct Submission
  JOURNAL   Submitted (24-MAR-2023) Environmental Microbiology, Helmholtz
            Center for Environmental Research -UFZ, Permoserstr 15, Leipzig
            04318, Germany
COMMENT     The annotation was added by the NCBI Prokaryotic Genome Annotation
            Pipeline (PGAP). Information about PGAP can be found here:
            https://www.ncbi.nlm.nih.gov/genome/annotation_prok/
            
            ##Genome-Assembly-Data-START##
            Assembly Method        :: SPAdes v. 3.11.1
            Genome Representation  :: Full
            Expected Final Version :: Yes
            Genome Coverage        :: Not Applicable
            Sequencing Technology  :: Illumina MiSeq
            ##Genome-Assembly-Data-END##
            
            ##Genome-Annotation-Data-START##
            Annotation Provider               :: NCBI
            Annotation Date                   :: 05/04/2023 04:57:10
            Annotation Pipeline               :: NCBI Prokaryotic Genome
                                                 Annotation Pipeline (PGAP)
            Annotation Method                 :: Best-placed reference protein
                                                 set; GeneMarkS-2+
            Annotation Software revision      :: 6.5
            Features Annotated                :: Gene; CDS; rRNA; tRNA; ncRNA
            Genes (total)                     :: 2,776
            CDSs (total)                      :: 2,750
            Genes (coding)                    :: 2,714
            CDSs (with protein)               :: 2,714
            Genes (RNA)                       :: 26
            tRNAs                             :: 24
            ncRNAs                            :: 2
            Pseudo Genes (total)              :: 36
            CDSs (without protein)            :: 36
            Pseudo Genes (ambiguous residues) :: 0 of 36
            Pseudo Genes (frameshifted)       :: 2 of 36
            Pseudo Genes (incomplete)         :: 32 of 36
            Pseudo Genes (internal stop)      :: 2 of 36
            ##Genome-Annotation-Data-END##
FEATURES             Location/Qualifiers
     source          1..2948
                     /organism="Micropruina sp."
                     /mol_type="genomic DNA"
                     /submitter_seqid="NODE_13594_length_2948_cov_4.978223"
                     /isolate="AMR_MDS_2195"
                     /isolation_source="Activated sludge of municipal
                     wastewater treatment plants"
                     /db_xref="taxon:2737536"
                     /environmental_sample
                     /geo_loc_name="Canada"
                     /lat_lon="49.25 N 123.1 W"
                     /collection_date="2015-06-16"
                     /metagenome_source="wastewater metagenome"
                     /note="metagenomic"
     gene            <1..789
                     /locus_tag="PLF56_11795"
     CDS             <1..789
                     /locus_tag="PLF56_11795"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_004810980.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="aconitase family protein"
                     /protein_id="HOQ54280.1"
                     /translation="EMSMEGRMTICNMSIEWGAKAGMVAPDDTTFAYLKGRPHAPEGE
                     DWDAAVAYWRTLYTDDDAVFDVEVDIDADQLTPFVTWGTNPGQGVPLGGAVPSPDDFT
                     DPVEKSSAQRALEYMALEAGTPMRDIRVDTVFLGSCTNGRIEDLRLAASIIKGRRVAD
                     GVRMLVVPGSARVRLQAMDEGLDQIFKDAGAEWREAGCSMCLGMNPDQLAPGERSAST
                     SNRNFEGRQGKGGRTHLVSPPVAAATAVRGTLSSPADLPAPVNA"
     gene            800..1405
                     /gene="leuD"
                     /locus_tag="PLF56_11800"
     CDS             800..1405
                     /gene="leuD"
                     /locus_tag="PLF56_11800"
                     /EC_number="4.2.1.33"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_013862971.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_component: GO:0009316 - 3-isopropylmalate dehydratase
                     complex [Evidence IEA];
                     GO_function: GO:0003861 - 3-isopropylmalate dehydratase
                     activity [Evidence IEA];
                     GO_process: GO:0009098 - leucine biosynthetic process
                     [Evidence IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="3-isopropylmalate dehydratase small subunit"
                     /protein_id="HOQ54281.1"
                     /translation="MDKFETHTGVVVPLRRSNVDTDQIIPAVYLKRVTRTGFEDGLFA
                     AWRNDPEFVLNNPAYAAGTILVPGPDFGTGSSREHAVWALQNYGFKVVIGSRFGDIFR
                     SNSGKAGLLIAAVPQETVEALWQYTEANPGAELTVSLADRTITAGDAVYDFTIDDYTR
                     HRLLEGLDDIGLTLQQEHLITAYEARRPSWLPKTLPVRTAS"
     gene            1591..2367
                     /locus_tag="PLF56_11805"
     CDS             1591..2367
                     /locus_tag="PLF56_11805"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_002520170.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="lysophospholipid acyltransferase family protein"
                     /protein_id="HOQ54282.1"
                     /translation="MSRAELSLSTVNRAPVEPLFRVLAHTVAPILRLFTKQDWRGGDK
                     LPRTGSVIVVANHISNFDPVALGHFLIWHGRWPRTLAKSELWQVPVIGWLAASLGQIP
                     VERRTVRARESLAAAAAALSRGECVVIYPEGTITADPDTWPMTGRAGTARLALETGAP
                     VIPIGQWGAHQVMPGKKVVFPRLLPRRTMQILVGDPVPLDDLREAHDSPTAAAEASVR
                     IMDAITALVGQLREQTPPSGRYDLRSGRRLPRDWASEASQ"
     gene            complement(2466..>2948)
                     /locus_tag="PLF56_11810"
     CDS             complement(2466..>2948)
                     /locus_tag="PLF56_11810"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_016669267.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="short-chain dehydrogenase"
                     /protein_id="HOQ54283.1"
                     /translation="LPYSTAKFGAVGFSDGLSAELVGTGVTATTLVPGLMRTGSHLQA
                     QFFGDAAREYAWFATAASLPLVSMDARRAARTMVDGVLAGKPFVSTTPLSWLAIRFRG
                     LAPATTTRLMKLVAQLLPGATGDSETVPGAAARADLNSELVNALTTLGDRAAEANNEQ
                     "
BASE COUNT          478 a         1015 c         1007 g          448 t
ORIGIN      
        1 gagatgagca tggaaggtcg catgacgatc tgcaacatgt cgatcgagtg gggtgcgaag
       61 gccggcatgg ttgcccccga cgacaccacc ttcgcttacc tcaagggtcg tccccacgca
      121 cccgagggcg aggattggga cgccgcagtg gcctactggc gcaccctgta caccgacgac
      181 gacgccgtct tcgacgtcga ggtcgacatc gacgccgacc agctcacccc gttcgtcacc
      241 tggggcacca accccggcca gggtgtgccg ttgggtggcg cggtgccctc accggacgat
      301 ttcaccgacc cggtcgagaa gtcgtccgcc cagcgcgcac tcgaatacat ggcgctcgag
      361 gccggcaccc cgatgcgcga catccgcgtc gacaccgtct tcctgggctc gtgcaccaac
      421 ggacgcatcg aggacctgcg gctcgcggcg tccatcatca agggacgccg ggtggccgac
      481 ggcgtccgca tgctggtggt gccgggctcg gcccgggtcc ggctgcaggc catggacgag
      541 ggcctcgacc agatcttcaa ggacgccggc gccgagtggc gtgaggcggg ttgttcgatg
      601 tgcctgggca tgaaccccga tcaactggca ccgggggagc gctcggcgtc cacctcgaac
      661 cgcaacttcg aaggccgcca gggcaagggc ggacgcaccc acctggtgtc gccgccggtg
      721 gccgccgcga ccgccgtccg aggcactttg tcgtcaccgg ccgacctgcc cgcaccagtc
      781 aacgcctgag gagcacgcga tggacaagtt cgaaacccac accggcgtcg tggtgccgct
      841 gcgccgtagc aatgtcgaca ccgaccagat cattcccgcc gtgtacctca agcgggtcac
      901 ccgcaccggg ttcgaggacg ggctgttcgc agcctggcgc aacgaccccg agttcgtgct
      961 gaacaatccg gcgtatgcgg ccggcacgat cctggtgccc gggcccgact tcggcaccgg
     1021 atcctcgcgc gagcacgccg tatgggcact gcagaactac ggcttcaagg tcgtgatcgg
     1081 ctcacggttc ggcgacatct tccgctcgaa ctcgggcaag gccgggctgt tgatcgccgc
     1141 cgtgccgcaa gagaccgtcg aggcgctgtg gcagtacacg gaggcgaatc cgggcgccga
     1201 actgaccgtg agcctggctg accggacgat caccgccggc gacgccgtct acgacttcac
     1261 catcgacgac tacacccggc accgcctgct cgagggtctg gacgacatcg gcctgaccct
     1321 gcagcaggag cacctgatca ccgcctacga ggcgcgccgt ccgtcctggc tgccgaagac
     1381 ccttccggtg cgcaccgcga gctgaatggg actgccccgc cgagggtgag tctccacatt
     1441 ctcgtggcaa cgtagcctga ctaaccttcg gttgttacag aaggcccggt cggaggcact
     1501 tttgttgcag ttcggacacg gccagccctc cgcgtcgcgt cgcggcgaag gcgccagtag
     1561 cctgattcga tcccaccctg gaggagttcg atgtcccgcg ccgaattgtc gctgtcgacg
     1621 gtgaaccgtg cgcccgtgga gccgctgttc cgcgtgttgg cgcacactgt tgcgccgatc
     1681 cttcgcctgt tcacgaaaca ggactggcgc gggggtgaca agctcccgcg aaccggttcg
     1741 gtgattgtgg tcgcgaacca catctcgaac ttcgacccgg tcgcgctcgg tcatttcctg
     1801 atctggcacg ggcgctggcc ccgcaccctg gccaagagcg agctgtggca ggtgccggtg
     1861 atcgggtggc tggcggcgtc gctgggtcag atcccggtcg agcgacgcac cgtccgtgcc
     1921 cgtgagtcgc tggccgccgc cgcggcggcc ctgagccgtg gggaatgcgt ggtgatctac
     1981 ccggagggca cgatcacggc cgaccctgac acatggccga tgaccggacg tgccggcacg
     2041 gcacggttgg cactcgagac cggcgctccg gtgatcccga tcggccagtg gggtgcccat
     2101 caggtgatgc cgggcaagaa ggtggtgttt ccccgcctgc taccccggcg caccatgcag
     2161 atcctggtgg gagatccggt tccattggac gacctgcgcg aggcgcacga ctcgccgacg
     2221 gccgccgccg aggcgagcgt gcgcatcatg gacgcgatca ccgcgctggt cggccagttg
     2281 cgcgaacaga ctccaccgag cggacgatac gacctacggt ccggacggcg gctgccgcgc
     2341 gattgggcca gcgaagcgtc gcagtgagcg gacgccgggc ccgggtgagg cgggcagccg
     2401 gacgccgatc aggtcgtacg cagtcgggag agctcaggtg gtgcgccggg atgacggcag
     2461 ccccctcact gctcgttgtt ggcctccgcc gcacggtcgc cgagggtggt cagcgcgttc
     2521 accagttccg agttcagatc cgcgcgcgca gcggcgccgg gaaccgtctc ggagtcgccg
     2581 gttgcccccg gcagcagctg cgcaaccagc ttcatcagcc tggtggtggt ggccggtgcc
     2641 aggccgcgga accggatcgc caaccagctc agcggtgtgg tgctgacgaa cggcttgccg
     2701 gccaacacgc cgtccaccat cgttcgggcc gcccgacggg cgtccatgga aaccagcggc
     2761 agcgaggcgg cggtggcgaa ccaggcgtac tcgcgcgcgg cgtcgccgaa aaactgcgcc
     2821 tgcagatgcg accccgtccg catcaatccg gggacaagcg tggtggcggt caccccggtg
     2881 cccaccagtt cggccgagag gccgtcggag aacccgaccg caccgaactt ggccgtcgaa
     2941 tagggcag
//
LOCUS       DAOSUG010000403         2935 bp    DNA     linear   ENV 10-JUN-2024
DEFINITION  MAG TPA_asm: Micropruina sp. isolate AMR_MDS_2195
            NODE_13667_length_2935_cov_3.814931, whole genome shotgun sequence.
ACCESSION   DAOSUG010000403 DAOSUG010000000
VERSION     DAOSUG010000403.1
DBLINK      BioProject: PRJNA925477
            BioSample: SAMN32864262
            Sequence Read Archive: SRR4236673
KEYWORDS    WGS; ENV; Metagenome Assembled Genome; MAG; Third Party Data; TPA;
            TPA:assembly.
SOURCE      Micropruina sp. (wastewater metagenome)
  ORGANISM  Micropruina sp.
            Bacteria; Bacillati; Actinomycetota; Actinomycetes;
            Propionibacteriales; Nocardioidaceae; Micropruina.
REFERENCE   1  (bases 1 to 2935)
  AUTHORS   Abdulkadir,N., Saraiva,J.P., Zhang,J., Stolte,S., Gillor,O.,
            Harms,H. and Rocha,U.
  TITLE     Genome-centric analyses of 165 metagenomes show that mobile genetic
            elements are crucial for the transmission of antimicrobial
            resistance genes to pathogens in activated sludge and wastewater
  JOURNAL   Microbiol Spectr 12 (3), e0291823 (2024)
   PUBMED   38289113
REFERENCE   2  (bases 1 to 2935)
  AUTHORS   Abdulkadir,N.
  TITLE     Direct Submission
  JOURNAL   Submitted (24-MAR-2023) Environmental Microbiology, Helmholtz
            Center for Environmental Research -UFZ, Permoserstr 15, Leipzig
            04318, Germany
COMMENT     The annotation was added by the NCBI Prokaryotic Genome Annotation
            Pipeline (PGAP). Information about PGAP can be found here:
            https://www.ncbi.nlm.nih.gov/genome/annotation_prok/
            
            ##Genome-Assembly-Data-START##
            Assembly Method        :: SPAdes v. 3.11.1
            Genome Representation  :: Full
            Expected Final Version :: Yes
            Genome Coverage        :: Not Applicable
            Sequencing Technology  :: Illumina MiSeq
            ##Genome-Assembly-Data-END##
            
            ##Genome-Annotation-Data-START##
            Annotation Provider               :: NCBI
            Annotation Date                   :: 05/04/2023 04:57:10
            Annotation Pipeline               :: NCBI Prokaryotic Genome
                                                 Annotation Pipeline (PGAP)
            Annotation Method                 :: Best-placed reference protein
                                                 set; GeneMarkS-2+
            Annotation Software revision      :: 6.5
            Features Annotated                :: Gene; CDS; rRNA; tRNA; ncRNA
            Genes (total)                     :: 2,776
            CDSs (total)                      :: 2,750
            Genes (coding)                    :: 2,714
            CDSs (with protein)               :: 2,714
            Genes (RNA)                       :: 26
            tRNAs                             :: 24
            ncRNAs                            :: 2
            Pseudo Genes (total)              :: 36
            CDSs (without protein)            :: 36
            Pseudo Genes (ambiguous residues) :: 0 of 36
            Pseudo Genes (frameshifted)       :: 2 of 36
            Pseudo Genes (incomplete)         :: 32 of 36
            Pseudo Genes (internal stop)      :: 2 of 36
            ##Genome-Annotation-Data-END##
FEATURES             Location/Qualifiers
     source          1..2935
                     /organism="Micropruina sp."
                     /mol_type="genomic DNA"
                     /submitter_seqid="NODE_13667_length_2935_cov_3.814931"
                     /isolate="AMR_MDS_2195"
                     /isolation_source="Activated sludge of municipal
                     wastewater treatment plants"
                     /db_xref="taxon:2737536"
                     /environmental_sample
                     /geo_loc_name="Canada"
                     /lat_lon="49.25 N 123.1 W"
                     /collection_date="2015-06-16"
                     /metagenome_source="wastewater metagenome"
                     /note="metagenomic"
     gene            <1..1101
                     /gene="rho"
                     /locus_tag="PLF56_11815"
     CDS             <1..1101
                     /gene="rho"
                     /locus_tag="PLF56_11815"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_016669769.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_function: GO:0004386 - helicase activity [Evidence
                     IEA];
                     GO_function: GO:0008186 - ATP-dependent activity, acting
                     on RNA [Evidence IEA];
                     GO_process: GO:0006353 - DNA-templated transcription
                     termination [Evidence IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="transcription termination factor Rho"
                     /protein_id="HOQ54284.1"
                     /translation="DVLDNYAFVRTSGYLPGNNDAYVSLSMVRKFGLRKGDIVTGAIR
                     QPREGERKEKFNPMVRIDTINADAPEKARDRAEFGKLTPLYPDERLKLETSAVGMIGR
                     IIDLVSPVGKGQRGLIVSPPKAGKTLIMQAIANAITTNNPEVHLMVVLVDERPEEVTD
                     FQRSVKGEVIASTFDRPADDHTTIAELAIERAKRLVELGRDVVILLDGITRLGRAYNL
                     AAPASGRILSGGVDSAALYPPKKFFGAARNIEDGGSLTILATALIETGSKMDEVIFEE
                     FKGTGNMELRLRRDLADKRIFPAIDVDASGTRREEMLFTREELQIIWKLRRVLSSLEP
                     QAALETLLSRMRKTESNREFLIQVTRTTPSQN"
     gene            1234..>2935
                     /locus_tag="PLF56_11820"
     CDS             1234..>2935
                     /locus_tag="PLF56_11820"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_020391879.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="AAA family ATPase"
                     /protein_id="HOQ54285.1"
                     /translation="MPASDPLLEARAHLDGARTALRGMREHTASLEALAGDRLSGEYL
                     KQALYRRMMSLRDDPATPLFFGRIDYRTTVGADENENCWIGRLHVTREFGSEPMVYDW
                     RARVSLPFYRASRTEPMGVDLRRRFGFQQGAVTAFEDERLAGDTSATDDDAFGRSHIL
                     EAEIERPRTGPMRDIVATIQPEQDLIVRAPLAESVVVQGAPGTGKTAVGLHRAAYLLY
                     AYRDQLSRSGVMVVGPNASFLSYIGDVLPALGEIDAAQETLESVVTRSAGRPVAGTDP
                     ADAAVLKGDARLAEVLRRAVWHSIVDPTDTLVVPRGSAQWRIPAHRCQAVIDELRGRG
                     VRYAAGREMLAHRLAHQVLLRMEASGETPDDRVQTAVARSRPVKAYVDAHWPALDAAK
                     VVFRLLSDAGFRAQHAGGLLSDAEQSLLDLTHGLTGRHRAPRSVAAWKPSLADLVLID
                     EVADLLNRTPSLGHVIVDEAQDLSAMGLRAIGRRASTGSVTVLGDLAQATTPYATTSW
                     SDSLTHLGQRTSTVTELVAGFRVPRSVIEYAARLLPSIAPSLTPPHSVRRSAGDFRIG
                     I"
BASE COUNT          465 a         1027 c         1000 g          443 t
ORIGIN      
        1 gacgtgctcg acaactacgc gttcgtgcgc acctcgggct atctgccggg caacaacgac
       61 gcctacgtgt cgctgtccat ggtgcgcaag ttcggcctgc gcaagggcga catcgtcacc
      121 ggcgccatcc ggcagccgcg cgagggtgag cgcaaagaga agttcaaccc catggtgcgc
      181 atcgacacca tcaacgccga cgcgcccgag aaggcccgtg accgagccga gttcggcaag
      241 ctgaccccgc tgtaccccga cgagcggctc aagctcgaaa cctcggcggt cggcatgatc
      301 gggcgcatca tcgacctggt cagcccggtc ggcaagggcc agcgcggcct gatcgtctcg
      361 ccgcccaagg ccggcaagac gctgatcatg caggcgatcg ccaacgccat caccaccaac
      421 aaccccgaag tgcacctgat ggtggtgctg gtcgacgagc gtcccgaaga ggtcaccgac
      481 tttcagcgca gcgtgaaggg cgaggtgatc gcctccacgt tcgaccgtcc ggccgacgat
      541 cacaccacga tcgccgaact ggccatcgag cgggccaagc gcctggtcga actgggtcgt
      601 gacgtggtga tcctgctcga cggcatcacc cggctcggcc gtgcgtacaa cctggccgcc
      661 ccggcgtccg gacgcatcct gtccggcggt gtcgactccg cggcgctgta cccgccgaag
      721 aagttcttcg gcgcggcccg caacatcgag gacggcggct cgctgaccat tctcgccacg
      781 gccctgatcg agaccggctc gaagatggat gaggtgatct tcgaagagtt caagggcacc
      841 ggcaacatgg agttgcggct gcgccgtgac ctcgccgaca agcgcatctt cccggccatc
      901 gacgtggatg cctccggcac ccgccgcgaa gagatgctgt tcacccgcga ggagcttcag
      961 atcatctgga agctgcgccg ggtgctgtcg agcctcgaac cgcaggccgc actggagacg
     1021 ttgctgtcgc gtatgcgcaa gaccgagagc aaccgtgagt tcctgattca ggtcacccgc
     1081 accacgccgt cgcagaactg acgacccgcc cagcgccccc ctaagtatct ctggcctgcg
     1141 aagcttcgcg ggccctggaa agttaggggg gcgttcggcg tggggccgcg ggcgtatgct
     1201 gaacggctcg cccacgactt cggaggtttc gccgtgcccg cgtccgatcc gctgctcgag
     1261 gcccgcgcgc atctggacgg ggcgcgcacg gcgttgcgcg gcatgcgcga gcacacggcg
     1321 tccctggagg cactggccgg ggaccggctg agcggtgagt acctgaaaca ggcgctctat
     1381 cggcgcatga tgtcgctgcg cgacgacccg gccacgcccc tgttcttcgg acgcatcgac
     1441 taccgcacca cggtcggcgc cgacgagaac gagaactgct ggatcggacg cctgcacgtc
     1501 acccgcgagt tcggcagcga gccgatggtg tacgactggc gggcgcgagt gtccctgccg
     1561 ttctaccggg ccagccgcac cgaaccgatg ggtgtcgatc ttcgtcgccg cttcggcttt
     1621 cagcagggtg ccgtcaccgc gttcgaggac gagaggctgg ccggcgacac ctccgcaact
     1681 gacgacgacg ccttcggccg ctcacacatt ctcgaggccg agatcgaacg cccccgcacc
     1741 gggccgatgc gcgacatcgt ggccacgatc cagcccgagc aggacctgat cgtgcgtgcg
     1801 ccgctggccg agtcggtcgt cgtgcagggc gcaccgggca ccggcaagac ggcggtcgga
     1861 ctgcaccgcg ccgcctacct gctgtacgcc taccgcgacc aactcagccg cagcggcgtc
     1921 atggtggtcg gaccgaacgc gtcctttctg tcctatatcg gcgacgtgct gccggcgctg
     1981 ggcgagatcg atgccgcgca ggagacgttg gagtccgtcg tcacccgcag cgccggacgt
     2041 ccggtggccg gcaccgaccc cgccgacgcc gccgtgctca agggcgacgc ccggctggcc
     2101 gaggtactgc gtcgtgcggt gtggcactcg atcgtggacc cgaccgacac cctggtggtg
     2161 ccgcgcggct cggcgcagtg gcgcattccg gcgcatcgct gccaggcggt gatcgacgag
     2221 ctacgcgggc ggggggtgcg ttacgcggcc ggacgcgaga tgctcgccca tcggctcgcc
     2281 caccaggtgt tgctgcgcat ggaggcatcg ggggagacgc ccgatgatcg ggtgcagacc
     2341 gcggtcgcgc ggtcgcgtcc ggtgaaggcg tacgtggacg cgcactggcc ggcactggac
     2401 gccgccaagg tcgtcttccg actattgagc gacgcgggct tccgggccca gcacgccggc
     2461 ggtttgctga gcgacgccga acagtcgctg ctcgatctga cccacgggct gaccggccgg
     2521 caccgcgcgc cccgttcggt cgcggcctgg aagccgagcc tggccgacct ggtgctgatc
     2581 gacgaggtgg ccgacctgct caaccgcaca ccgagtcttg gccacgtgat cgtcgacgag
     2641 gcgcaggacc tctccgcgat ggggctgcgc gcgatcggac gacgggccag caccggctcg
     2701 gtgaccgtgc tcggcgacct ggcccaggcc accacgccct atgcgaccac cagttggtcc
     2761 gactcgctga cccatctcgg ccagcggacc agcacggtca ccgagttggt cgccgggttt
     2821 cgggtgccgc gctcggtgat cgagtacgcg gcccggctgt tgcccagcat cgcgccgtcg
     2881 ttgaccccgc cgcattcggt gcgacggtcg gcgggcgact tccggattgg gatcg
//
LOCUS       DAOSUG010000404         2928 bp    DNA     linear   ENV 10-JUN-2024
DEFINITION  MAG TPA_asm: Micropruina sp. isolate AMR_MDS_2195
            NODE_13714_length_2928_cov_3.259659, whole genome shotgun sequence.
ACCESSION   DAOSUG010000404 DAOSUG010000000
VERSION     DAOSUG010000404.1
DBLINK      BioProject: PRJNA925477
            BioSample: SAMN32864262
            Sequence Read Archive: SRR4236673
KEYWORDS    WGS; ENV; Metagenome Assembled Genome; MAG; Third Party Data; TPA;
            TPA:assembly.
SOURCE      Micropruina sp. (wastewater metagenome)
  ORGANISM  Micropruina sp.
            Bacteria; Bacillati; Actinomycetota; Actinomycetes;
            Propionibacteriales; Nocardioidaceae; Micropruina.
REFERENCE   1  (bases 1 to 2928)
  AUTHORS   Abdulkadir,N., Saraiva,J.P., Zhang,J., Stolte,S., Gillor,O.,
            Harms,H. and Rocha,U.
  TITLE     Genome-centric analyses of 165 metagenomes show that mobile genetic
            elements are crucial for the transmission of antimicrobial
            resistance genes to pathogens in activated sludge and wastewater
  JOURNAL   Microbiol Spectr 12 (3), e0291823 (2024)
   PUBMED   38289113
REFERENCE   2  (bases 1 to 2928)
  AUTHORS   Abdulkadir,N.
  TITLE     Direct Submission
  JOURNAL   Submitted (24-MAR-2023) Environmental Microbiology, Helmholtz
            Center for Environmental Research -UFZ, Permoserstr 15, Leipzig
            04318, Germany
COMMENT     The annotation was added by the NCBI Prokaryotic Genome Annotation
            Pipeline (PGAP). Information about PGAP can be found here:
            https://www.ncbi.nlm.nih.gov/genome/annotation_prok/
            
            ##Genome-Assembly-Data-START##
            Assembly Method        :: SPAdes v. 3.11.1
            Genome Representation  :: Full
            Expected Final Version :: Yes
            Genome Coverage        :: Not Applicable
            Sequencing Technology  :: Illumina MiSeq
            ##Genome-Assembly-Data-END##
            
            ##Genome-Annotation-Data-START##
            Annotation Provider               :: NCBI
            Annotation Date                   :: 05/04/2023 04:57:10
            Annotation Pipeline               :: NCBI Prokaryotic Genome
                                                 Annotation Pipeline (PGAP)
            Annotation Method                 :: Best-placed reference protein
                                                 set; GeneMarkS-2+
            Annotation Software revision      :: 6.5
            Features Annotated                :: Gene; CDS; rRNA; tRNA; ncRNA
            Genes (total)                     :: 2,776
            CDSs (total)                      :: 2,750
            Genes (coding)                    :: 2,714
            CDSs (with protein)               :: 2,714
            Genes (RNA)                       :: 26
            tRNAs                             :: 24
            ncRNAs                            :: 2
            Pseudo Genes (total)              :: 36
            CDSs (without protein)            :: 36
            Pseudo Genes (ambiguous residues) :: 0 of 36
            Pseudo Genes (frameshifted)       :: 2 of 36
            Pseudo Genes (incomplete)         :: 32 of 36
            Pseudo Genes (internal stop)      :: 2 of 36
            ##Genome-Annotation-Data-END##
FEATURES             Location/Qualifiers
     source          1..2928
                     /organism="Micropruina sp."
                     /mol_type="genomic DNA"
                     /submitter_seqid="NODE_13714_length_2928_cov_3.259659"
                     /isolate="AMR_MDS_2195"
                     /isolation_source="Activated sludge of municipal
                     wastewater treatment plants"
                     /db_xref="taxon:2737536"
                     /environmental_sample
                     /geo_loc_name="Canada"
                     /lat_lon="49.25 N 123.1 W"
                     /collection_date="2015-06-16"
                     /metagenome_source="wastewater metagenome"
                     /note="metagenomic"
     gene            <1..338
                     /locus_tag="PLF56_11825"
     CDS             <1..338
                     /locus_tag="PLF56_11825"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_016668356.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=3
                     /transl_table=11
                     /product="cation transporter"
                     /protein_id="HOQ54286.1"
                     /translation="PRAITILREAGSILLESTPPGLNLEEVRRHILEVPHVVGVHDLH
                     ASTIATGLPVLTAHVVVEEACFADGHAAQMLTDLQACVADHFDVSVEHSTFQLESPDH
                     AQREHTTHT"
     gene            405..740
                     /locus_tag="PLF56_11830"
     CDS             405..740
                     /locus_tag="PLF56_11830"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_018784511.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_component: GO:0016020 - membrane [Evidence IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="YnfA family protein"
                     /protein_id="HOQ54287.1"
                     /translation="MDTLRSVALFIAAAIAEIGGAWLVWQGVREHRGWIWIGAGVIAL
                     GLYGFVATLQPDPNFGRILAAYGGVFVAGSLAWGMIFDGFRPDRYDIAGALMCLIGVA
                     VIMYSPRAA"
     gene            complement(869..2221)
                     /locus_tag="PLF56_11835"
     CDS             complement(869..2221)
                     /locus_tag="PLF56_11835"
                     /EC_number="1.-.-.-"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_011755108.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="NAD(P)/FAD-dependent oxidoreductase"
                     /protein_id="HOQ54288.1"
                     /translation="MTPGYDLIVIGAGMAGVTAANKCAAAGWRVAIVDALPYGGTCAL
                     RGCDPKKILRRGAEIIDSARLMRGKGIDAAGLSINWADLIQHKHGFTDPVPRRMEDGL
                     SGNGVDALHGQARFTGPNTMAIEGISYHAAHFLIAAGARPRPLEFHGHEHLVDSTDFM
                     ELPELPRRILFVGGGFISFEFAHIAARAGSTPVILDRGPRPLKAFDPDLVELLISRGG
                     EVGIELWRSTTITAIEKSRRGFQVSVERSGQSETVGFDLVVHGAGRVPELAGLNLDAA
                     AVEWDVSGVRVAPHLQSTSNAAVYAAGDAANTPGMPLTPVAVFEGKVAASNMLKASTT
                     VPDYSGVPTAVFTIPELARVGMLEQEARDQGIDIDVRYSDTSGWYSNYRIGETTAATK
                     ILVDRSSDRIVGAHLLGPEYGEVVNLIALAMKLNLTTSQLKSMTAAYPTVGSDLSSIL
                     "
     gene            complement(2221..2532)
                     /locus_tag="PLF56_11840"
     CDS             complement(2221..2532)
                     /locus_tag="PLF56_11840"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_013864598.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="thioredoxin family protein"
                     /protein_id="HOQ54289.1"
                     /translation="MDVSLLYFDDCPNWKIAARRLDAIAAESPGVVVSHRRVETLEDA
                     ERLRFLGSPSILVDGVDVFAGPGSSVGLSCRIYRTPDGMAGVPSLEQLRGALGLSREE
                     A"
     gene            2588..>2928
                     /locus_tag="PLF56_11845"
     CDS             2588..>2928
                     /locus_tag="PLF56_11845"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_011755106.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="heavy metal-responsive transcriptional
                     regulator"
                     /protein_id="HOQ54290.1"
                     /translation="MLIGELALATGMSRQTIRFYERKGLLPEASRGHNGYRVYDDSTL
                     ARLRFVNLAQAAGMTLSEIHRIIELREDGIVPCAHVVTLIDNKLVEVRARIRHLAALQ
                     AELEGLLQHSRL"
BASE COUNT          498 a          980 c          932 g          518 t
ORIGIN      
        1 tgccgcgggc gatcaccatc ctgcgggagg cggggagcat cctgctggag tccactcctc
       61 cggggttgaa cctcgaagag gtccgtcgtc acatcctgga ggtgccgcac gtggtggggg
      121 tgcacgacct gcacgcttcc accatcgcca ccggtctgcc ggtcctcacc gcccacgttg
      181 tggtcgagga ggcctgcttc gccgacggcc acgccgccca gatgctgact gatctccaag
      241 cgtgtgtggc cgaccacttc gacgtgagcg tcgagcactc caccttccaa ctcgaatcac
      301 ccgaccacgc gcaacgcgaa cacaccacac acacctagct cccccaaccc gcaggccagg
      361 ggtacacagc tcttggcgac actccgagct gggaaacttg caccatggac acactcagat
      421 cagtcgcctt gttcatcgcg gcagccatcg ccgagatcgg cggagcctgg ctcgtctggc
      481 aaggagtacg cgaacaccgc ggctggatct ggatcggtgc cggcgttatc gccctcggcc
      541 tgtacggctt cgtcgccacc ctgcaacccg accccaactt cggtcgcatc ctcgccgcct
      601 acggcggcgt cttcgtcgcc ggctccctcg cctggggcat gatcttcgac ggcttccgtc
      661 ccgaccgcta cgacatcgcc ggcgccctca tgtgcctgat cggcgtcgcc gtcatcatgt
      721 actccccacg cgccgcctga ccccagtccc acgcgcatct tctgagacgg cgatgaccag
      781 accggcagtg tggtggccgt ttccagcgtc aaggatctgg ctcggtgacc ggaactgctc
      841 gccgtggcgc cccctgtgcc gggccaagtc agaggatcga gctgaggtct gacccgacgg
      901 tgggataggc agcagtcatt gacttcagtt gactggtggt caggttgagc ttcatggcga
      961 gggcgatgag attgacgacc tcgccgtact cggggccgag aaggtgcgct ccgacaatcc
     1021 ggtcgctgga tcggtcgaca aggatcttgg tggctgcggt ggtctcaccg atccggtagt
     1081 tggagtacca gccactggtg tcgctgtagc ggacgtcgat gtcgatgcct tgatcgcggg
     1141 cttcttgttc gagcatcccg acccgagcca gttcggggat cgtgaagacc gccgtgggga
     1201 caccgctgta gtcggggacg gtcgtcgagg ccttgagcat gttggacgcg gcgaccttgc
     1261 cctcgaacac cgcgactggg gtcagcggca tccccggggt gttggcggcg tcgccggcgg
     1321 cgtagacggc tgcgttgctg gtgctctgca ggtgcggagc gacccgcact ccacttacgt
     1381 cccactcgac cgcggcggcg tccaggttga ggccggccag ctccgggact cggccggctc
     1441 cgtgcacgac gaggtcgaac cccaccgtct cggattgacc ggatcgctcc acactgacct
     1501 gaaaccctct ccgggatttc tcgatcgcgg tgatcgtggt ggagcgccac aactcgatcc
     1561 caacctcacc gccgcggctg atcaggagtt cgacgaggtc ggggtcgaac gccttgagcg
     1621 gccttggccc gcggtcgagg atgaccgggg tgctgcccgc gcgtgccgcg atgtgggcga
     1681 actcgaagga gatgaaaccg ccaccgacga acaggatccg tcgcggcaat tcggggagct
     1741 ccatgaagtc ggtgctgtcg accaggtgtt cgtgaccgtg gaattccagt ggccgggggc
     1801 gagcgccggc ggcgatcagg aagtgtgcgg cgtggtagga gatgccctcg atcgccatgg
     1861 tgttcggtcc agtgaaccgc gcctggccgt gcagcgcgtc cactccgttg ccggagagtc
     1921 cgtcctccat ccttctcggg accgggtcgg tgaagccgtg cttgtgctgg atcaagtcgg
     1981 cccagttgat cgacaggccg gcagcgtcga tccctttgcc ccgcatcagt cgggcgctgt
     2041 cgatgatctc ggcaccgcga cgcaggatct tcttcgggtc gcacccacgc agtgcgcagg
     2101 tgccgccgta cggaagcgcg tcgacgatcg cgacgcgcca gcccgcggca gcacacttgt
     2161 tcgccgcagt cacaccagcc atgcccgcgc cgatcacgat caggtcatag cctggcgtca
     2221 tcacgcctcc tcgcgcgaca gaccgagcgc tccccggagt tgctccagtg agggcacccc
     2281 cgccatcccg tcaggggtcc ggtagatccg gcaggacaat cccacgctcg agcctggccc
     2341 agcgaagaca tccacaccgt cgaccaggat gctcggcgag ccgaggaacc gcagtcgttc
     2401 tgcgtcttcc agcgtttcga cgcggcgatg gcttactacg acccccggtg actcggcagc
     2461 gatcgcgtca agtcgtcggg cggcgatctt ccagttcggg cagtcgtcga agtacaacag
     2521 tgagacgtcc atgtccacga cggtaaacct tgtaccgtgg tagaaggtca agtagggggc
     2581 ggaagagatg ttgatcggtg agcttgccct ggcaactggg atgagcaggc aaaccattcg
     2641 gttctatgaa cgcaagggcc tcctgcccga ggcgtcccgg ggccacaacg gctaccgcgt
     2701 ctacgacgat tcgacgctcg cccggttgcg cttcgtcaat ctggcgcaag cagcaggcat
     2761 gacactgtcc gagatccacc gcatcatcga gcttcgcgag gacgggattg tcccctgtgc
     2821 gcacgtggtc acgctcatcg acaacaagct ggtcgaagtc cgggcacgca tcagacacct
     2881 tgccgccctg caagcagaac tcgaaggact actccaacac agccgcct
//
LOCUS       DAOSUG010000405         2921 bp    DNA     linear   ENV 10-JUN-2024
DEFINITION  MAG TPA_asm: Micropruina sp. isolate AMR_MDS_2195
            NODE_13759_length_2921_cov_4.506281, whole genome shotgun sequence.
ACCESSION   DAOSUG010000405 DAOSUG010000000
VERSION     DAOSUG010000405.1
DBLINK      BioProject: PRJNA925477
            BioSample: SAMN32864262
            Sequence Read Archive: SRR4236673
KEYWORDS    WGS; ENV; Metagenome Assembled Genome; MAG; Third Party Data; TPA;
            TPA:assembly.
SOURCE      Micropruina sp. (wastewater metagenome)
  ORGANISM  Micropruina sp.
            Bacteria; Bacillati; Actinomycetota; Actinomycetes;
            Propionibacteriales; Nocardioidaceae; Micropruina.
REFERENCE   1  (bases 1 to 2921)
  AUTHORS   Abdulkadir,N., Saraiva,J.P., Zhang,J., Stolte,S., Gillor,O.,
            Harms,H. and Rocha,U.
  TITLE     Genome-centric analyses of 165 metagenomes show that mobile genetic
            elements are crucial for the transmission of antimicrobial
            resistance genes to pathogens in activated sludge and wastewater
  JOURNAL   Microbiol Spectr 12 (3), e0291823 (2024)
   PUBMED   38289113
REFERENCE   2  (bases 1 to 2921)
  AUTHORS   Abdulkadir,N.
  TITLE     Direct Submission
  JOURNAL   Submitted (24-MAR-2023) Environmental Microbiology, Helmholtz
            Center for Environmental Research -UFZ, Permoserstr 15, Leipzig
            04318, Germany
COMMENT     The annotation was added by the NCBI Prokaryotic Genome Annotation
            Pipeline (PGAP). Information about PGAP can be found here:
            https://www.ncbi.nlm.nih.gov/genome/annotation_prok/
            
            ##Genome-Assembly-Data-START##
            Assembly Method        :: SPAdes v. 3.11.1
            Genome Representation  :: Full
            Expected Final Version :: Yes
            Genome Coverage        :: Not Applicable
            Sequencing Technology  :: Illumina MiSeq
            ##Genome-Assembly-Data-END##
            
            ##Genome-Annotation-Data-START##
            Annotation Provider               :: NCBI
            Annotation Date                   :: 05/04/2023 04:57:10
            Annotation Pipeline               :: NCBI Prokaryotic Genome
                                                 Annotation Pipeline (PGAP)
            Annotation Method                 :: Best-placed reference protein
                                                 set; GeneMarkS-2+
            Annotation Software revision      :: 6.5
            Features Annotated                :: Gene; CDS; rRNA; tRNA; ncRNA
            Genes (total)                     :: 2,776
            CDSs (total)                      :: 2,750
            Genes (coding)                    :: 2,714
            CDSs (with protein)               :: 2,714
            Genes (RNA)                       :: 26
            tRNAs                             :: 24
            ncRNAs                            :: 2
            Pseudo Genes (total)              :: 36
            CDSs (without protein)            :: 36
            Pseudo Genes (ambiguous residues) :: 0 of 36
            Pseudo Genes (frameshifted)       :: 2 of 36
            Pseudo Genes (incomplete)         :: 32 of 36
            Pseudo Genes (internal stop)      :: 2 of 36
            ##Genome-Annotation-Data-END##
FEATURES             Location/Qualifiers
     source          1..2921
                     /organism="Micropruina sp."
                     /mol_type="genomic DNA"
                     /submitter_seqid="NODE_13759_length_2921_cov_4.506281"
                     /isolate="AMR_MDS_2195"
                     /isolation_source="Activated sludge of municipal
                     wastewater treatment plants"
                     /db_xref="taxon:2737536"
                     /environmental_sample
                     /geo_loc_name="Canada"
                     /lat_lon="49.25 N 123.1 W"
                     /collection_date="2015-06-16"
                     /metagenome_source="wastewater metagenome"
                     /note="metagenomic"
     gene            complement(<1..281)
                     /locus_tag="PLF56_11850"
     CDS             complement(<1..281)
                     /locus_tag="PLF56_11850"
                     /inference="COORDINATES: ab initio
                     prediction:GeneMarkS-2+"
                     /note="Derived by automated computational analysis using
                     gene prediction method: GeneMarkS-2+."
                     /codon_start=1
                     /transl_table=11
                     /product="hypothetical protein"
                     /protein_id="HOQ54291.1"
                     /translation="MTSRGRRAPLATALTCLALGASLALAGCSGSAPANSAAVPTDAA
                     KITGELNILVSSASGSDAGFKAVNEAFAKKYPDVKINFTAVPNENYNQAR"
     gene            complement(447..1559)
                     /locus_tag="PLF56_11855"
     CDS             complement(447..1559)
                     /locus_tag="PLF56_11855"
                     /inference="COORDINATES: protein motif:HMM:NF012742.2"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_function: GO:0000976 - transcription cis-regulatory
                     region binding [Evidence IEA];
                     GO_function: GO:0003700 - DNA-binding transcription factor
                     activity [Evidence IEA];
                     GO_function: GO:0043565 - sequence-specific DNA binding
                     [Evidence IEA];
                     GO_process: GO:0006351 - DNA-templated transcription
                     [Evidence IEA];
                     GO_process: GO:0006355 - regulation of DNA-templated
                     transcription [Evidence IEA];
                     GO_process: GO:0045892 - negative regulation of
                     DNA-templated transcription [Evidence IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="LacI family DNA-binding transcriptional
                     regulator"
                     /protein_id="HOQ54292.1"
                     /translation="MPTRMSGPTPTRRPAPRGEVRVSITDVAREAGVAISTVSAALNG
                     RTGVSEPTRERIAGIAERLGWVPSMRGRSLVAQRAWAVGLLVQRPASVLEADPFFAGF
                     IGGVETVLDDAGYALLLQLASSRERVLRRVPQWAHSEVVDGIFLTDVQSDDPRFEVID
                     RVGLPAVAINCASHPGVSTVAQQHQPGLVALLQHLLELGHTRIAHVGGPPGFVHSAER
                     EAVWRATLAAAGVDPGPLVAGDFTSEGGARAADLLLATDDPPTAVVCANDLAAIGFIS
                     RAGTLGFVVPAQISVTGFDGIQLSGFTSPPLTTVQTSPHDLGAEAARLLLHLIGTGEV
                     LHHEIPPAQLLLRASTAPPRPPAPRRRAPALRRALP"
     gene            1647..>2921
                     /locus_tag="PLF56_11860"
     CDS             1647..>2921
                     /locus_tag="PLF56_11860"
                     /inference="COORDINATES: protein motif:HMM:NF013591.2"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_function: GO:0004222 - metalloendopeptidase activity
                     [Evidence IEA];
                     GO_process: GO:0006508 - proteolysis [Evidence IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="M3 family metallopeptidase"
                     /protein_id="HOQ54293.1"
                     /translation="MALSALVLPHTDWLGWLGHRGDGQLATARERVAAAVALPPGDPE
                     ILQLFNQASIALRNAGSVAGLLSSVHPDAAVIERAEALEIEVQRFSTDLYLDPAIYAA
                     ISSVAADGLDADARRLQAKVAQSFRRSGVDRDESVRQRVRELEATLTELGQTFSRNIR
                     DGRSETRVPATALDGLPDDFAQSHPADPDGLVTITTEYPDVMPFLAFATDAAARRAVA
                     HTFFNIAWPGNDAVLADLLRVRHELATLLGYATWADYDAEVKMIGQGAAIAEFIDTVA
                     SDAQASGERELGVLRARAERDGESVIDVSNWRYYHELIKREQYGVDATQVRRYFDFGK
                     VHDGLLDITARLFGLRFTPVDAQQARTWHPDVTSYDVAYAEGGDPIGRIHLDLHPRER
                     KYNHAAQFELVAGVREVQLPEGVLVCNFPRGLM"
BASE COUNT          444 a         1020 c         1029 g          428 t
ORIGIN      
        1 cgggcctggt tgtagttctc gttgggtacc gcggtgaagt tgatcttcac gtcggggtac
       61 ttcttggcga aagcctcgtt gacggccttg aacccggcgt cagagccgga ggcgctggag
      121 accagaatgt tgagttcgcc ggtgatcttg gcggcatcgg tcggcacggc cgccgagttg
      181 gccggtgcac tgccggaaca accggccagt gcgagcgaag cccctagtgc aaggcaggtc
      241 agggcggtgg caagtggtgc gcggcgaccg cgcgacgtca tcgtcgacat ggggtccctc
      301 cgggtatccg aaacgtttcg gtgattgaag ataacaccgc gtcggcaaga tggcgagacc
      361 ccggtgttga aatctcgcac cggccccccg ccccgaacgc tcgcctaacc atcgtgcgcc
      421 tgcgaagctt cgcgggcgca cgatggttag gggagcgctc ggcgtagggc cggggcgcgt
      481 cggcgtgggg ccgggggtcg gggtggcgcg gtggatgccc gcagcagcag ctgcgcgggg
      541 ggtatctcgt ggtgcaggac ctccccggtg ccgatcagat gcagcagcag cctggcggcc
      601 tcggcgccca agtcgtgggg gcttgtctgc accgtggtca gcggtgggct ggtgaacccc
      661 gacaactgaa tgccgtcgaa gcccgtcacc gagatctgcg ccggtacgac gaaccccagg
      721 gtgccggccc gcgagatgaa cccgatcgcc gccaggtcgt tcgcacagac caccgccgtc
      781 ggcgggtcgt ccgtggcgag cagcagatca gcggcccggg cacccccctc ggacgtgaaa
      841 tcgcccgcca ccagcgggcc gggatccacc cccgcggcgg ccagagtggc gcgccacacc
      901 gcctcgcgct cggcggaatg cacgaagccc ggcggcccgc ccacatgggc gatgcgggtg
      961 tggcccagtt cgagcagatg ttgcagcagg gcgaccaggc cgggctgatg ctgctgggcg
     1021 acggtcgaca ccccgggatg gctcgcacag ttgatcgcca ccgccggcag cccgacccgg
     1081 tcgatcacct cgaagcgcgg atcgtccgac tgcacgtcgg tcaggaagat gccgtcgacc
     1141 acctcgctgt gcgcccactg cggcacccgg cgcagcaccc gctcgcgcga ggacgccagc
     1201 tgcagcagca gggcgtagcc ggcgtcgtcc aggacggtct cgacaccgcc gatgaagccg
     1261 gcgaagaagg ggtccgcttc gagcaccgag gccggacgtt gcaccagcag tcccaccgcc
     1321 cacgcgcgct gggcgaccag gctgcgtcca cgcatggacg gcacccagcc cagccgttcg
     1381 gcgatgcccg cgatgcgttc gcgcgtgggt tcgctcacgc cggtgcgtcc gttgagggcc
     1441 gccgagacgg tgctgatggc cacccccgcc tcgcgggcca cgtcggtgat gctcacccgc
     1501 acctcgccac gcggcgcggg acgccgggtc ggggtcgggc cgctcatccg ggtgggcatg
     1561 ggcagatact gccaccgcaa cgccgtggtg cccagcgcgg tgtcggcccg acctgactgt
     1621 cggtgccgcc ccgtagggtg cagggcatgg ctctttcagc attggttctg ccccacaccg
     1681 attggctcgg ctggctcggc catcgcggcg acgggcagtt ggccaccgcg cgcgaacgcg
     1741 tcgccgccgc ggtggccctg ccgcccggcg accccgagat actccaactc ttcaaccagg
     1801 caagcatcgc gctgcgtaac gccggcagcg tggcgggcct gctgtcgtcg gtgcatcctg
     1861 acgctgcggt gatcgagcgc gccgaggccc tcgagatcga ggtgcaacgg ttcagcaccg
     1921 acctgtacct cgacccggcc atctacgcgg cgatcagctc ggtggcggcc gacgggctcg
     1981 acgcggacgc ccgccggctt caggccaagg ttgcgcagtc gttccggcgc tccggggtcg
     2041 atcgtgacga gtccgtgcgg cagcgcgtgc gcgagctgga ggccacgctg accgagttgg
     2101 gccagacctt cagccgcaac attcgcgacg gacggtccga gacccgggtg cccgcaacgg
     2161 cgctcgacgg cctgcccgac gacttcgcgc agtcgcatcc agccgaccca gacgggctgg
     2221 tcacgatcac caccgagtac cccgacgtga tgccctttct ggccttcgcc accgacgccg
     2281 cggcccgccg tgcggtggcg cacacgttct tcaacatcgc ctggccaggc aacgacgcgg
     2341 tgctcgccga cctgctgcgg gtgcgccacg aactggccac cctgttgggt tacgccacct
     2401 gggccgacta cgacgccgag gtcaagatga tcggccaggg ggccgccatc gccgagttca
     2461 tcgacaccgt cgcgtccgac gcgcaggcct cgggtgagcg tgagttgggc gtgctgcggg
     2521 cgcgggccga acgcgacggc gagagcgtca tcgatgtctc gaactggcgc tactaccacg
     2581 agctgatcaa gcgcgagcag tacggggtcg acgccaccca ggtgcgccga tacttcgact
     2641 tcggcaaggt gcacgacgga ctgctcgaca tcaccgcgcg cctgttcggg ctgcggttca
     2701 cgccggtcga tgcccagcag gcgcgaacct ggcaccccga cgtgaccagc tacgacgtgg
     2761 cttacgccga gggcggtgac ccgatcggac gcattcacct cgacctgcac ccacgcgagc
     2821 gcaagtacaa ccacgcggcc cagttcgagt tggtcgccgg cgtgcgcgag gtgcagttgc
     2881 ccgagggtgt gctggtctgc aactttcccc gcggtctgat g
//
LOCUS       DAOSUG010000406         2913 bp    DNA     linear   ENV 10-JUN-2024
DEFINITION  MAG TPA_asm: Micropruina sp. isolate AMR_MDS_2195
            NODE_13807_length_2913_cov_3.908328, whole genome shotgun sequence.
ACCESSION   DAOSUG010000406 DAOSUG010000000
VERSION     DAOSUG010000406.1
DBLINK      BioProject: PRJNA925477
            BioSample: SAMN32864262
            Sequence Read Archive: SRR4236673
KEYWORDS    WGS; ENV; Metagenome Assembled Genome; MAG; Third Party Data; TPA;
            TPA:assembly.
SOURCE      Micropruina sp. (wastewater metagenome)
  ORGANISM  Micropruina sp.
            Bacteria; Bacillati; Actinomycetota; Actinomycetes;
            Propionibacteriales; Nocardioidaceae; Micropruina.
REFERENCE   1  (bases 1 to 2913)
  AUTHORS   Abdulkadir,N., Saraiva,J.P., Zhang,J., Stolte,S., Gillor,O.,
            Harms,H. and Rocha,U.
  TITLE     Genome-centric analyses of 165 metagenomes show that mobile genetic
            elements are crucial for the transmission of antimicrobial
            resistance genes to pathogens in activated sludge and wastewater
  JOURNAL   Microbiol Spectr 12 (3), e0291823 (2024)
   PUBMED   38289113
REFERENCE   2  (bases 1 to 2913)
  AUTHORS   Abdulkadir,N.
  TITLE     Direct Submission
  JOURNAL   Submitted (24-MAR-2023) Environmental Microbiology, Helmholtz
            Center for Environmental Research -UFZ, Permoserstr 15, Leipzig
            04318, Germany
COMMENT     The annotation was added by the NCBI Prokaryotic Genome Annotation
            Pipeline (PGAP). Information about PGAP can be found here:
            https://www.ncbi.nlm.nih.gov/genome/annotation_prok/
            
            ##Genome-Assembly-Data-START##
            Assembly Method        :: SPAdes v. 3.11.1
            Genome Representation  :: Full
            Expected Final Version :: Yes
            Genome Coverage        :: Not Applicable
            Sequencing Technology  :: Illumina MiSeq
            ##Genome-Assembly-Data-END##
            
            ##Genome-Annotation-Data-START##
            Annotation Provider               :: NCBI
            Annotation Date                   :: 05/04/2023 04:57:10
            Annotation Pipeline               :: NCBI Prokaryotic Genome
                                                 Annotation Pipeline (PGAP)
            Annotation Method                 :: Best-placed reference protein
                                                 set; GeneMarkS-2+
            Annotation Software revision      :: 6.5
            Features Annotated                :: Gene; CDS; rRNA; tRNA; ncRNA
            Genes (total)                     :: 2,776
            CDSs (total)                      :: 2,750
            Genes (coding)                    :: 2,714
            CDSs (with protein)               :: 2,714
            Genes (RNA)                       :: 26
            tRNAs                             :: 24
            ncRNAs                            :: 2
            Pseudo Genes (total)              :: 36
            CDSs (without protein)            :: 36
            Pseudo Genes (ambiguous residues) :: 0 of 36
            Pseudo Genes (frameshifted)       :: 2 of 36
            Pseudo Genes (incomplete)         :: 32 of 36
            Pseudo Genes (internal stop)      :: 2 of 36
            ##Genome-Annotation-Data-END##
FEATURES             Location/Qualifiers
     source          1..2913
                     /organism="Micropruina sp."
                     /mol_type="genomic DNA"
                     /submitter_seqid="NODE_13807_length_2913_cov_3.908328"
                     /isolate="AMR_MDS_2195"
                     /isolation_source="Activated sludge of municipal
                     wastewater treatment plants"
                     /db_xref="taxon:2737536"
                     /environmental_sample
                     /geo_loc_name="Canada"
                     /lat_lon="49.25 N 123.1 W"
                     /collection_date="2015-06-16"
                     /metagenome_source="wastewater metagenome"
                     /note="metagenomic"
     gene            199..1449
                     /locus_tag="PLF56_11865"
     CDS             199..1449
                     /locus_tag="PLF56_11865"
                     /inference="COORDINATES: protein motif:HMM:NF014998.2"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_function: GO:0004144 - diacylglycerol O-acyltransferase
                     activity [Evidence IEA];
                     GO_process: GO:0045017 - glycerolipid biosynthetic process
                     [Evidence IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="wax ester/triacylglycerol synthase family
                     O-acyltransferase"
                     /protein_id="HOQ54294.1"
                     /translation="MVDPVSRNEALLLARDQPVQPWHQVSVLILDGPALDAADVQRLI
                     AQRLAYSPRFRQKLAGTGLQSWIDDRGFHLAGHVRSEPLSGRLETRLEALLDTPLERL
                     HPLWDALVLDGLPGQRWALVVRAHPALVDGRDNVHLLHELLDEQPTLIDGDVPPWQPA
                     IEEPTGFGGLLRGVSDPMQALRDAAAGIGGLAENGLRQVSTESSPRSVAAVEFDLADL
                     VRVARAAGCTVHDVVVALVTAGVRGGQLDAGEDLHDPVALVPLAVDDDGASAMGCLVA
                     PQFIQLPVTAPTADVRLDAIATLTQARIDSDRLVSARELVEVAGFAPPTVHAVAASQV
                     TSGRAHEVLIANVPGPSEARWLGERRVRALHAFESTVDEQRLTVGVTSLNGRIGFAAT
                     ALQPLPKFARTVGDELGVLQRMVG"
     gene            1449..1913
                     /locus_tag="PLF56_11870"
     CDS             1449..1913
                     /locus_tag="PLF56_11870"
                     /inference="COORDINATES: ab initio
                     prediction:GeneMarkS-2+"
                     /note="Derived by automated computational analysis using
                     gene prediction method: GeneMarkS-2+."
                     /codon_start=1
                     /transl_table=11
                     /product="hypothetical protein"
                     /protein_id="HOQ54295.1"
                     /translation="MLVFVPLTAKELAGWAGSGRFVPTPAFAVTASLRQAFGFTPDDD
                     EDAGHTALHIAGLAGLLQHRTRLVVVAETSARAVSAHEFGEFGEVDVGELPWGAVTAL
                     FGDEASVASDELRRRLQGLSLATAWDEPDVADFLAEHELSWHGPEEWPVLAG"
     gene            1953..2417
                     /locus_tag="PLF56_11875"
     CDS             1953..2417
                     /locus_tag="PLF56_11875"
                     /inference="COORDINATES: protein motif:HMM:TIGR00689.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="RpiB/LacA/LacB family sugar-phosphate isomerase"
                     /protein_id="HOQ54296.1"
                     /translation="MRIAMANDHAGTALKFDVAAWLSAEGHDVVDFGAADEAACDLPD
                     HVYPAALAVAGGDCERGIFVDGVGYGSAMIANKLPGVFAAVAQDPFCAWLARSHSNTN
                     VLCIGAKIIGSALAMEVVKAWITTDWLGTTEPKYARRVAKVVEIAERHVRPL"
     gene            2494..>2913
                     /locus_tag="PLF56_11880"
     CDS             2494..>2913
                     /locus_tag="PLF56_11880"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_013864112.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="Ku protein"
                     /protein_id="HOQ54297.1"
                     /translation="MPRSIWKGAISFGLITIPIKVYGATETKDLSFRQVHAEDGGRIK
                     YKRVCEVCGKEIPYPEIAKGYETGDGRLVILEAEDFENLPLPTAKAVEVVQFVKSEDV
                     DPTYFERTYFLEADGPGAKPYVLLRQALAQEGKAAVVK"
BASE COUNT          426 a          975 c         1072 g          440 t
ORIGIN      
        1 cacagagtga cgaacagcgg ggtggacggg cgacgacggg taggcaccgg gtgattcttg
       61 cacgacgcgc gcccgggttg aggcgcggcg gcacgtaggc tggcccggta cgtcgaaccc
      121 ccgcctaagt atcgtgcgcc cgcgtacctt cgcaggccag agatggttag gggggcggtc
      181 gcgaggaaag gacccgccgt ggtcgatccg gtcagccgaa acgaggcact gctgctggcg
      241 cgtgaccagc cggtgcagcc ctggcatcag gtcagcgtgc tgattctgga cggtccggcg
      301 ttggacgctg ccgacgtgca acgcctgatc gcccagcggc tggcctactc gccgcggttc
      361 cggcagaagc tggccggcac gggcctgcag tcctggatcg acgaccgggg ctttcacctc
      421 gccggccatg tgcgctccga gccgctgagc ggacggctcg aaacccggct cgaggccctg
      481 ctggacaccc cgctggagcg gctgcatccg ctatgggacg ccctggtgct ggacggactg
      541 ccggggcagc gctgggcgct ggtggtgcgg gcccacccgg cgctggtcga cggccgcgac
      601 aacgtccacc tgctgcatga gctgctggac gagcagccca cactgatcga cggcgacgtg
      661 ccgccctggc agccggcgat cgaggaaccc accggcttcg gcggcctgtt gcggggcgtc
      721 tcggacccga tgcaggccct gcgcgacgcc gcggccggca tcgggggcct ggccgagaac
      781 ggcctgcggc aggtgagcac cgaatcgtcc ccacgatcgg tggcggccgt ggagttcgac
      841 ctggccgacc tggtccgggt ggcgcgcgcg gccgggtgca cggtgcacga cgtggtggtg
      901 gccctcgtca cagcaggggt gcggggcggc caactggacg ccggtgagga cctgcacgac
      961 ccggtcgcgc tggtgccgct ggccgtggac gacgacgggg ccagcgccat gggctgcctg
     1021 gtcgcgccgc agttcatcca gttgccggtg accgcgccga ccgcggacgt ccgactggac
     1081 gcgatcgcga cgctgaccca ggcgcggatc gattccgacc ggctggtgtc ggcccgtgag
     1141 ctggtcgagg tggccgggtt cgcgccgccc acggtgcatg ccgttgcggc gagccaggtg
     1201 accagcggac gcgcccacga ggtgctgatc gccaacgttc ccggaccgtc cgaggcccgc
     1261 tggctgggtg agcggcgggt ccgggcgctg cacgccttcg aatccaccgt ggacgagcaa
     1321 cggctcacgg tcggggtgac cagcctgaac ggacggatcg gcttcgccgc caccgcactg
     1381 cagccgctgc cgaaattcgc ccgcaccgtg ggcgacgaac tgggcgtgct gcaacggatg
     1441 gtcggctgat gctggtcttc gtgccgctca ccgcgaaaga gctggcgggc tgggccggta
     1501 gcggccgatt cgtaccgacg ccggccttcg ccgtcactgc ttcgctgcgg caggccttcg
     1561 gcttcacccc cgatgacgat gaggatgccg ggcacaccgc gctgcatatt gccggtctcg
     1621 ccggcctgct ccagcaccgg acgcggttgg tcgtggtcgc ggaaacctcc gctcgcgccg
     1681 tctcggcgca cgagttcggc gagttcggcg aggtcgacgt gggggagttg ccctggggcg
     1741 cggtcaccgc cctgttcggg gacgaggcct cggtggcctc cgacgagctc cgccggcgtc
     1801 tgcagggttt gtccttggcc acggcctggg acgagcccga cgtcgccgac ttcctagccg
     1861 agcacgagtt gtcgtggcac ggccccgaag aatggcccgt cctggccggt tgaggcctgg
     1921 gttctgtcgt ggggccgcga tagcgtgacc ccatgcggat cgcgatggcc aacgaccatg
     1981 ccggcacggc gctcaagttc gacgtcgccg cttggctgag cgccgagggc cacgatgtgg
     2041 tcgacttcgg ggccgccgat gaggcggcct gcgatctgcc cgatcacgtc tatccggcgg
     2101 cactggccgt ggccggcggc gactgcgagc gcggcatctt cgtcgacggc gtcggctacg
     2161 gctcggcgat gatcgccaac aagctgccgg gcgtgttcgc ggcggtggcg caagacccgt
     2221 tctgtgcgtg gctggcccgc tcgcactcca acaccaacgt gctctgcatc ggcgctaaga
     2281 tcatcggttc ggcgctggcc atggaggtgg tcaaggcctg gatcaccacc gactggctgg
     2341 gtaccaccga gccgaagtac gcccggcggg tggcgaaggt ggtcgagatc gccgagcggc
     2401 acgtgcgtcc gctgtgaccc cggccgcacc acccaccctg gcgtgacttc ggacgatgag
     2461 gcaggctagg ccaaacgcag ggtaggagga agcatgccgc ggtccatctg gaagggcgcg
     2521 atctcgttcg gcctgatcac cattccgatc aaggtctacg gcgccaccga gaccaaggat
     2581 ctctcgtttc gccaggtgca cgccgaggac ggcgggcgga tcaagtacaa gcgggtctgt
     2641 gaggtctgcg gcaaggagat tccctacccc gagatcgcca agggctacga gaccggcgac
     2701 gggcgcctgg tgattctcga ggcagaggac ttcgagaacc tgccgctgcc caccgccaag
     2761 gcggtcgagg tcgtccagtt cgtcaagagc gaggacgtcg acccgaccta cttcgaacgc
     2821 acctactttc tcgaggcgga cggccccggc gccaagccct acgtgctgct gcgacaggcg
     2881 ctcgcgcagg agggaaaggc ggccgtggtc aag
//
LOCUS       DAOSUG010000407         2904 bp    DNA     linear   ENV 10-JUN-2024
DEFINITION  MAG TPA_asm: Micropruina sp. isolate AMR_MDS_2195
            NODE_13860_length_2904_cov_3.248508, whole genome shotgun sequence.
ACCESSION   DAOSUG010000407 DAOSUG010000000
VERSION     DAOSUG010000407.1
DBLINK      BioProject: PRJNA925477
            BioSample: SAMN32864262
            Sequence Read Archive: SRR4236673
KEYWORDS    WGS; ENV; Metagenome Assembled Genome; MAG; Third Party Data; TPA;
            TPA:assembly.
SOURCE      Micropruina sp. (wastewater metagenome)
  ORGANISM  Micropruina sp.
            Bacteria; Bacillati; Actinomycetota; Actinomycetes;
            Propionibacteriales; Nocardioidaceae; Micropruina.
REFERENCE   1  (bases 1 to 2904)
  AUTHORS   Abdulkadir,N., Saraiva,J.P., Zhang,J., Stolte,S., Gillor,O.,
            Harms,H. and Rocha,U.
  TITLE     Genome-centric analyses of 165 metagenomes show that mobile genetic
            elements are crucial for the transmission of antimicrobial
            resistance genes to pathogens in activated sludge and wastewater
  JOURNAL   Microbiol Spectr 12 (3), e0291823 (2024)
   PUBMED   38289113
REFERENCE   2  (bases 1 to 2904)
  AUTHORS   Abdulkadir,N.
  TITLE     Direct Submission
  JOURNAL   Submitted (24-MAR-2023) Environmental Microbiology, Helmholtz
            Center for Environmental Research -UFZ, Permoserstr 15, Leipzig
            04318, Germany
COMMENT     The annotation was added by the NCBI Prokaryotic Genome Annotation
            Pipeline (PGAP). Information about PGAP can be found here:
            https://www.ncbi.nlm.nih.gov/genome/annotation_prok/
            
            ##Genome-Assembly-Data-START##
            Assembly Method        :: SPAdes v. 3.11.1
            Genome Representation  :: Full
            Expected Final Version :: Yes
            Genome Coverage        :: Not Applicable
            Sequencing Technology  :: Illumina MiSeq
            ##Genome-Assembly-Data-END##
            
            ##Genome-Annotation-Data-START##
            Annotation Provider               :: NCBI
            Annotation Date                   :: 05/04/2023 04:57:10
            Annotation Pipeline               :: NCBI Prokaryotic Genome
                                                 Annotation Pipeline (PGAP)
            Annotation Method                 :: Best-placed reference protein
                                                 set; GeneMarkS-2+
            Annotation Software revision      :: 6.5
            Features Annotated                :: Gene; CDS; rRNA; tRNA; ncRNA
            Genes (total)                     :: 2,776
            CDSs (total)                      :: 2,750
            Genes (coding)                    :: 2,714
            CDSs (with protein)               :: 2,714
            Genes (RNA)                       :: 26
            tRNAs                             :: 24
            ncRNAs                            :: 2
            Pseudo Genes (total)              :: 36
            CDSs (without protein)            :: 36
            Pseudo Genes (ambiguous residues) :: 0 of 36
            Pseudo Genes (frameshifted)       :: 2 of 36
            Pseudo Genes (incomplete)         :: 32 of 36
            Pseudo Genes (internal stop)      :: 2 of 36
            ##Genome-Annotation-Data-END##
FEATURES             Location/Qualifiers
     source          1..2904
                     /organism="Micropruina sp."
                     /mol_type="genomic DNA"
                     /submitter_seqid="NODE_13860_length_2904_cov_3.248508"
                     /isolate="AMR_MDS_2195"
                     /isolation_source="Activated sludge of municipal
                     wastewater treatment plants"
                     /db_xref="taxon:2737536"
                     /environmental_sample
                     /geo_loc_name="Canada"
                     /lat_lon="49.25 N 123.1 W"
                     /collection_date="2015-06-16"
                     /metagenome_source="wastewater metagenome"
                     /note="metagenomic"
     gene            56..481
                     /locus_tag="PLF56_11885"
     CDS             56..481
                     /locus_tag="PLF56_11885"
                     /EC_number="3.5.4.33"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_014847856.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_function: GO:0008270 - zinc ion binding [Evidence IEA];
                     GO_function: GO:0052717 - tRNA-specific adenosine-34
                     deaminase activity [Evidence IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="nucleoside deaminase"
                     /protein_id="HOQ54298.1"
                     /translation="MRLALAQAEGALAHRDVPIGAVVVGPEGQVVAAAHNERELTGDP
                     TAHAEVLALRRASEVTGSWRLAGHTLVVTLEPCTMCAGALVLARIERVVIGAWDPKAG
                     AVSSLFDVVRDPRLNHRVEVVAGVEAEACGALLRGFFGR"
     gene            521..610
                     /locus_tag="PLF56_11890"
     tRNA            521..610
                     /locus_tag="PLF56_11890"
                     /product="tRNA-Ser"
                     /inference="COORDINATES: profile:tRNAscan-SE:2.0.12"
                     /note="Derived by automated computational analysis using
                     gene prediction method: tRNAscan-SE."
                     /anticodon=(pos:555..557,aa:Ser,seq:cga)
     gene            complement(613..1548)
                     /locus_tag="PLF56_11895"
     CDS             complement(613..1548)
                     /locus_tag="PLF56_11895"
                     /inference="COORDINATES: protein motif:HMM:NF015431.2"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="LysR substrate-binding domain-containing
                     protein"
                     /protein_id="HOQ54299.1"
                     /translation="MVDVQRLRVYRAVVAAGSIQAAAANLGFTPSAVSQQVSALARET
                     GLVLLGKAGRGIEPTDAGLELIQAADNLFGELAEIEAKVADLREGRAGTLSMTYFTST
                     GMVWIPPIVQRLLQQHPRLRLDLRVREIPTGEDEHTDVEVLVAPVDFSAPPGFRSRPL
                     LTEPYLAVVPADHRLADRGVIELAELADERWIAHDPDLSWCVINLKQSCLAAGFTPHY
                     SVRTADHTSAIAFVAVGIGVTVMPRLCTIDLPPGVRTVEVVNPTPQRTIHAMVRWAVE
                     RTPAVQLVMEGLADMARAHADMQTRPAPLLAERTS"
     gene            complement(1594..1908)
                     /locus_tag="PLF56_11900"
     CDS             complement(1594..1908)
                     /locus_tag="PLF56_11900"
                     /inference="COORDINATES: ab initio
                     prediction:GeneMarkS-2+"
                     /note="Derived by automated computational analysis using
                     gene prediction method: GeneMarkS-2+."
                     /codon_start=1
                     /transl_table=11
                     /product="hypothetical protein"
                     /protein_id="HOQ54300.1"
                     /translation="MEILLIVALFAPVVYLMERTHRHTRDLPRAPFGFDAESEASSRY
                     RRQLAELRALSGQLGDAAAPTSAQRTPAVSAALTRLQRTLGASFTPLGGWSGTATTLK
                     HC"
     gene            2184..>2904
                     /locus_tag="PLF56_11905"
     CDS             2184..>2904
                     /locus_tag="PLF56_11905"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_017934315.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_function: GO:0016624 - oxidoreductase activity, acting
                     on the aldehyde or oxo group of donors, disulfide as
                     acceptor [Evidence IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="thiamine pyrophosphate-dependent enzyme"
                     /protein_id="HOQ54301.1"
                     /translation="MSDPAVDDFVQLLTAEGERVQHPEFAFDGSDEQVAGYLREMVLA
                     RRFDNEATALQRKGELGLWPPALGQEAAQVGSVNATRRPDHVFPSYREHAVALHLGID
                     PVEMLSTFRGTAMVPWNTVELGLHMYSFVIGTQTLHAVGYAMGLQRDGLVGNPDAATN
                     GATMVYFGDGATSQGDVNEAFIFAASYTAPVVFFCQNNQYAISEPMALQSKIPLYRRA
                     SGFGFPGVRVDGNDVLAVHAVT"
BASE COUNT          439 a          949 c         1050 g          466 t
ORIGIN      
        1 gtgcaggtct ccttcggcct gtgacggggc tccggtgaac cgctgggcac cggcgatgag
       61 gctcgcattg gcgcaggccg agggcgccct cgcgcatcgc gacgtgccga tcggggcggt
      121 ggtggtcggc cctgagggtc aggtcgtggc cgccgcgcac aacgagcgcg agttgaccgg
      181 cgacccgacg gcgcacgccg aggtgctggc gttgcgacgg gcgtccgagg tgacggggtc
      241 gtggcggctt gccgggcaca cgctggtggt caccctcgag ccatgcacga tgtgcgccgg
      301 ggcgctggtg ctggcccgta tcgagcgggt ggtgatcggt gcgtgggatc ccaaggcggg
      361 ggcggtgtcg tcgctgttcg acgtggtgcg cgacccgcgg ctgaaccatc gcgtcgaggt
      421 ggtcgccggc gtcgaggccg aggcctgcgg ggcgttgctg cgagggttct tcggccgctg
      481 atttggcacc cggacgcggc gcacggatag aatcgccgac ggtggcgtgt ccgagcggcc
      541 taaggagcac gcctcgaaag cgtgtgtggg tttatgccca ccgtgggttc aaatcccacc
      601 gccaccgcca gttcacgagg ttcgttccgc cagcagcgga gcgggcctcg tttgcatatc
      661 ggcatgggcc cgcgccatgt ctgccaggcc ctccatcacc agctggacgg ccggcgtgcg
      721 ctcgacggcc cagcgaacca tggcgtgaat ggtgcgctgc ggggtggggt tgaccacctc
      781 gacggtgcgc acgcccgggg gcaggtcgat ggtgcacagc cggggcatca cggtgacccc
      841 gatgccgacc gccacgaagg cgatcgccga ggtgtgatcg gcggtgcgca ccgagtagtg
      901 cggggtgaag cccgccgcca ggcacgattg cttgaggttg atcacgcacc aactcaggtc
      961 cggatcgtgg gcgatccatc gctcgtcggc cagttcggcc agttcgatca cgccgcgatc
     1021 ggccagccgg tgatcggccg gcaccaccgc gagatacggc tcggtgagaa gcggacgcga
     1081 ccggaaaccg ggcggcgcgg agaagtcgac gggtgcgacg agcacctcga cgtcggtgtg
     1141 ctcgtcctcg ccggtcggaa tctcgcggac ccgcaggtcg agtcgcagcc gcggatgctg
     1201 ctgcagcagc cgctgcacga tcgggggaat ccacaccatg ccggtcgagg tgaagtacgt
     1261 catgctgagc gtgcccgcgc ggccctcgcg caagtcggcc accttggcct cgatctcggc
     1321 gagttcgccg aacaggttgt cggccgcctg gatcagttcg agaccggcgt cggtgggctc
     1381 gatgccgcgg cccgccttgc ccagtagcac caggccggtt tcgcgagcca gcgccgagac
     1441 ctgctggctg accgcggacg gcgtgaaccc gaggttcgcg gcggccgcct ggatcgatcc
     1501 tgccgcgacg acggcccggt agacgcgaag tcgttgcacg tcgaccatgg cgctcacagt
     1561 atcaagcgcg agctccggat cggagctcac gcgtcagcag tgcttcagcg tggtggcagt
     1621 gcccgaccag ccgcccagcg gggtgaagct ggcgcccagg gtgcgctgca gcctggtcag
     1681 cgcagcggac acggcgggcg tccgctgcgc cgaagtgggg gcggcagcgt cgcccagttg
     1741 cccggacagc gcgcgcaact cggccagttg gcgccggtag cggctgctgg cttcggattc
     1801 ggcgtcgaaa ccgaacggtg cgcgaggcag gtcgcgggtg tggcggtggg tgcgttccat
     1861 caggtagacc acgggtgcga acagggccac gatgagcagg atttccatgt cggtcgactc
     1921 ctttccggag ggctcggtgc acccaccttc tcgccttcga agctttagca caagctcggt
     1981 tttcttcatc atcattcagc cttactgaat caatcgggcc agggctccag cggcccggct
     2041 tacggcgcgg ggcctcgacc ccgaggggcc gcgggtggag gatgggcatg tgtgcccaga
     2101 cctttgacct acggttacgt aaccagtaac ttggctgctc gaaccgagac cgtgccccgg
     2161 cccatggctc gaaaggaacg actgtgtccg accccgctgt ggacgatttc gtccaactgc
     2221 tgaccgccga gggcgagcgc gtccaacacc ccgagttcgc gttcgacggc agcgacgaac
     2281 aggtcgcggg ctacctgcgc gaaatggttc tggctcgccg gttcgacaac gaggcgaccg
     2341 cactgcaacg caagggcgag ctggggctgt ggccaccggc gctgggccag gaggcggctc
     2401 aggtaggctc ggtgaatgcg acccggcggc ccgaccacgt gtttccgtcc tatcgcgagc
     2461 atgcggtcgc cctgcacctg ggcatcgacc cggtcgagat gctgagcacg tttcgtggca
     2521 ccgcgatggt gccctggaac accgtcgaac tgggcctgca catgtacagc ttcgtgatcg
     2581 gcacccagac cctgcacgcg gtcggttacg ccatggggct gcagcgcgac ggcctggtcg
     2641 gcaaccccga cgccgcgacc aacggggcca ccatggtgta cttcggcgac ggggcgacca
     2701 gccagggcga cgtcaacgag gcgttcatct tcgccgccag ctacaccgcc cccgtggtgt
     2761 tcttctgcca gaacaaccag tacgccattt cggagccgat ggccctgcag tcgaagattc
     2821 cgctgtaccg gcgggcgtcc ggcttcggct ttcccggcgt tcgggtggac ggcaacgacg
     2881 tgctcgccgt ccacgcggtc accc
//
LOCUS       DAOSUG010000408         2889 bp    DNA     linear   ENV 10-JUN-2024
DEFINITION  MAG TPA_asm: Micropruina sp. isolate AMR_MDS_2195
            NODE_13944_length_2889_cov_3.232886, whole genome shotgun sequence.
ACCESSION   DAOSUG010000408 DAOSUG010000000
VERSION     DAOSUG010000408.1
DBLINK      BioProject: PRJNA925477
            BioSample: SAMN32864262
            Sequence Read Archive: SRR4236673
KEYWORDS    WGS; ENV; Metagenome Assembled Genome; MAG; Third Party Data; TPA;
            TPA:assembly.
SOURCE      Micropruina sp. (wastewater metagenome)
  ORGANISM  Micropruina sp.
            Bacteria; Bacillati; Actinomycetota; Actinomycetes;
            Propionibacteriales; Nocardioidaceae; Micropruina.
REFERENCE   1  (bases 1 to 2889)
  AUTHORS   Abdulkadir,N., Saraiva,J.P., Zhang,J., Stolte,S., Gillor,O.,
            Harms,H. and Rocha,U.
  TITLE     Genome-centric analyses of 165 metagenomes show that mobile genetic
            elements are crucial for the transmission of antimicrobial
            resistance genes to pathogens in activated sludge and wastewater
  JOURNAL   Microbiol Spectr 12 (3), e0291823 (2024)
   PUBMED   38289113
REFERENCE   2  (bases 1 to 2889)
  AUTHORS   Abdulkadir,N.
  TITLE     Direct Submission
  JOURNAL   Submitted (24-MAR-2023) Environmental Microbiology, Helmholtz
            Center for Environmental Research -UFZ, Permoserstr 15, Leipzig
            04318, Germany
COMMENT     The annotation was added by the NCBI Prokaryotic Genome Annotation
            Pipeline (PGAP). Information about PGAP can be found here:
            https://www.ncbi.nlm.nih.gov/genome/annotation_prok/
            
            ##Genome-Assembly-Data-START##
            Assembly Method        :: SPAdes v. 3.11.1
            Genome Representation  :: Full
            Expected Final Version :: Yes
            Genome Coverage        :: Not Applicable
            Sequencing Technology  :: Illumina MiSeq
            ##Genome-Assembly-Data-END##
            
            ##Genome-Annotation-Data-START##
            Annotation Provider               :: NCBI
            Annotation Date                   :: 05/04/2023 04:57:10
            Annotation Pipeline               :: NCBI Prokaryotic Genome
                                                 Annotation Pipeline (PGAP)
            Annotation Method                 :: Best-placed reference protein
                                                 set; GeneMarkS-2+
            Annotation Software revision      :: 6.5
            Features Annotated                :: Gene; CDS; rRNA; tRNA; ncRNA
            Genes (total)                     :: 2,776
            CDSs (total)                      :: 2,750
            Genes (coding)                    :: 2,714
            CDSs (with protein)               :: 2,714
            Genes (RNA)                       :: 26
            tRNAs                             :: 24
            ncRNAs                            :: 2
            Pseudo Genes (total)              :: 36
            CDSs (without protein)            :: 36
            Pseudo Genes (ambiguous residues) :: 0 of 36
            Pseudo Genes (frameshifted)       :: 2 of 36
            Pseudo Genes (incomplete)         :: 32 of 36
            Pseudo Genes (internal stop)      :: 2 of 36
            ##Genome-Annotation-Data-END##
FEATURES             Location/Qualifiers
     source          1..2889
                     /organism="Micropruina sp."
                     /mol_type="genomic DNA"
                     /submitter_seqid="NODE_13944_length_2889_cov_3.232886"
                     /isolate="AMR_MDS_2195"
                     /isolation_source="Activated sludge of municipal
                     wastewater treatment plants"
                     /db_xref="taxon:2737536"
                     /environmental_sample
                     /geo_loc_name="Canada"
                     /lat_lon="49.25 N 123.1 W"
                     /collection_date="2015-06-16"
                     /metagenome_source="wastewater metagenome"
                     /note="metagenomic"
     gene            complement(<1..712)
                     /locus_tag="PLF56_11910"
     CDS             complement(<1..712)
                     /locus_tag="PLF56_11910"
                     /inference="COORDINATES: protein motif:HMM:NF013828.2"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_function: GO:0005524 - ATP binding [Evidence IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="ATP-binding protein"
                     /protein_id="HOQ54302.1"
                     /translation="MTSLDIETKRKLREMGATPLLDAFTAQDEALVLGMGFSERIALI
                     VDEAHQAFTHSKVDGLIRRAGLRYPKADLRRLDLVEERGLDRSLIAQLGTCTFIERQQ
                     NVVFQGFTGSGKSYLGSALAKQACLHRIRAHYIRMPDLEEAWALARDKPQGQMKFLRK
                     YAAFTLLVIDEWLLDHPDESMRSMLLELLERRYDTTSTVFCTQYAKKDWHQRLGSGVH
                     ADAIMDRIVHNTIWIDTGN"
     gene            complement(709..2271)
                     /gene="istA"
                     /locus_tag="PLF56_11915"
     CDS             complement(709..2271)
                     /gene="istA"
                     /locus_tag="PLF56_11915"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_013349124.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_function: GO:0004803 - transposase activity [Evidence
                     IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="IS21 family transposase"
                     /protein_id="HOQ54303.1"
                     /translation="MVRKIRAKLVLQLRAEGLSGRAIAASQQMSRKSVTAVLDAADAA
                     GVAWDEVAGQSETEVYSRLFPGRGEHESVFTQPDWDLVHREMARAGVTLKLLHGEYTD
                     SCTANRTPAMGYDRFCKTYQRHVLVTGAASRVGHKAGQTVEVDWSGPTMELIDPATGE
                     VSRVYLFVACLPFSRYAFVWPTLDMKQDTWLRAHVAMFDALGGSVPRIVPDNLKTGVV
                     KHPREGEVVLNDAYREMAAHYSAAVLPGRVRHPKDKASVENTVAHVATWVIAALRTTT
                     FTSLPDLAAIGDRIEAYNAEPFQKRPGSRMSVFAAEERPLLKPLPQVAYEVSRWVYGR
                     TVARDGHVVWARNHYSVPFVHIGGKVDLRITDRVLEAYRGPERLTSHLLLPEGSAGNW
                     RTNDADLPAGDRYQPWDADRVREWAGRIGPAAVTVINRIFESVPIDEQGLDPALAVLR
                     LSRRYSADRVEAACRLALAGRVRSPRYAHLKPILDTGQDQLAALGTPRPEPAAEGGYV
                     RGADYYANGGAK"
BASE COUNT          485 a          970 c          923 g          511 t
ORIGIN      
        1 ggttgccggt gtcgatccag atggtgttgt ggacgatgcg gtccatgatc gcgtcagcgt
       61 ggaccccgga gcccaggcgt tggtgccagt ccttcttcgc gtattgggtg cagaagacgg
      121 tggaggtggt gtcgtagcga cgctcgagga gttccaggag catgctgcgc atgctctcgt
      181 cggggtggtc gaggagccat tcatcgatca cgagcagtgt gaacgcggcg tacttgcgga
      241 ggaacttcat ctggccttgt ggcttgtcac gggccagtgc ccaggcttct tcgaggtcgg
      301 gcatccggat gtagtgggcc cggatccggt ggaggcaggc ctgtttcgcc agcgcggagc
      361 cgaggtagga cttcccggat ccggtgaagc cttggaagac gacgttctgt tgtcgttcga
      421 tgaacgtgca ggtgccgagt tgggcgatga gggagcggtc gaggccgcgt tcctcgacca
      481 gatcgagacg acgcaggtcg gctttcgggt agcgcagtcc tgcccggcgg atcaggccgt
      541 cgaccttgga gtgggtgaag gcctggtgtg cctcgtcgac tatcagagcg atccgctccg
      601 agaagcccat gccgagcacg agtgcctcgt cctgggctgt gaaggcatcg agtagtggcg
      661 tggcgcccat ctcgcggagc ttgcgtttgg tttcgatgtc gaggctggtc acttggcacc
      721 accgttggcg tagtagtcgg cgccgcggac gtagccgccc tcggcggccg gctcagggcg
      781 tggtgtcccg agcgcggcga gttggtcttg tccggtgtcg aggatcggtt tcagatgcgc
      841 gtagcgagga gacctcaccc gtcctgccag agcgagccgg caggccgctt cgacccggtc
      901 ggccgagtag cggcgcgaca gccgcaacac cgccagggcc gggtcgaggc cctgctcgtc
      961 gatggggacg gactcgaaga tccggttgat caccgtcacc gccgccggcc cgatccggcc
     1021 tgcccattcc cggacccggt cagcgtccca gggctgatac ctgtcaccgg cggggaggtc
     1081 ggcgtcgttg gtgcgccagt tccctgccga gccttcgggc agcagcaggt gactcgtcag
     1141 gcgttcaggg ccgcggtagg cctccaggac ccgatcggtg atccgcaggt cgaccttgcc
     1201 tccgatgtgg acgaacggca ccgagtagtg gttcctggcc cagaccacat gcccgtcgcg
     1261 tgcgacggtg cggccgtaga cccagcggct gacctcgtag gcgacctgcg gcaacggctt
     1321 cagcaacggc cgctcctccg ccgcgaacac actcatccgc gagccgggac gcttctggaa
     1381 cggctcagcg ttgtaagcct cgatccggtc accgatcgct gcgaggtcgg gcagggacgt
     1441 gaacgtcgtg gtccgcagcg cggcgatcac ccaggtcgcg acatgcgcca ccgtgttttc
     1501 caccgaggcc ttgtccttag ggtggcgaac tcggccgggc agcaccgccg ccgagtagtg
     1561 cgccgccatc tcccggtagg cgtcgttgag gacgacctca ccctcccggg gatgcttgac
     1621 caccccggtc ttcaagttgt ccggcacgat cctgggcacc gacccaccca acgcgtcgaa
     1681 catcgccacg tgcgcccgca gccacgtgtc ctgcttcata tccagcgtcg gccagacgaa
     1741 cgcgtacctg ctgaacggca gacacgccac gaacaagtac accctcgaga cctcaccggt
     1801 ggccgggtcg atcagctcca tcgtcggacc cgaccagtcg acctcgaccg tctgcccagc
     1861 cttgtgaccc acccgagacg cggcgccagt gaccagcacg tgacgctggt aggtcttgca
     1921 gaaccggtca tagcccatcg ccggcgtcct gttcgcggtg cacgaatcgg tgtactcgcc
     1981 atgcagcaac ttgagcgtca ccccggccct ggccatctcg cgatggacca ggtcccaatc
     2041 cggttgggtg aacacactct catgctcccc acggcccggg aacaaccgtg agtacacctc
     2101 agtctcgctc tgccccgcga cctcatccca cgccaccccg gcagcatcag cggcatccag
     2161 caccgccgtg acgctcttgc gtgacatctg ctgcgaagcc gcgatcgccc gacctgacag
     2221 gccctcggcg cgcagctgca acaccagctt cgctctgatc tttcgtacca tcgagatact
     2281 ccttccgcct gcgccctata cgcacggcgg gaggagccta cgaaccccag gtggccccca
     2341 agcacgccac ccccggcccc cacacccacg aaccagacat cacgccactg gcccccaacc
     2401 acacgatcag tggaccccag tgacgctgat attcaccgtg acggggccgg aaaatttccg
     2461 gggccgtgga cggtgcggga aaatttcgca caccgtctct ccggtagcga cggtgatggc
     2521 aagttgcagg aaccgtcaca cccccccacc cgaacgctgc tggtgccgcc gattcgacgg
     2581 caccaggccc gcctcgtcgc aatgaggcac ggtctgggcg ccacatgccg gtgacgacgc
     2641 gggagttgaa ctgaactcga tccccttgcg ccgtgaatcc cgctcgggcg ttctgccgca
     2701 taaaatcaac gatctacggc atctgccctg tggataactc caccgcgata cgccgaccac
     2761 ctacaggttg cttagcactt tcgttagcac ttttcgcggt gaggactggc tcgtcaacgc
     2821 atgggactgc tgcgcggata ggtgacaggt tgtagtcacg ccgccagtgc gacggtcgtg
     2881 ttcatgatg
//
LOCUS       DAOSUG010000409         2887 bp    DNA     linear   ENV 10-JUN-2024
DEFINITION  MAG TPA_asm: Micropruina sp. isolate AMR_MDS_2195
            NODE_13958_length_2887_cov_5.109816, whole genome shotgun sequence.
ACCESSION   DAOSUG010000409 DAOSUG010000000
VERSION     DAOSUG010000409.1
DBLINK      BioProject: PRJNA925477
            BioSample: SAMN32864262
            Sequence Read Archive: SRR4236673
KEYWORDS    WGS; ENV; Metagenome Assembled Genome; MAG; Third Party Data; TPA;
            TPA:assembly.
SOURCE      Micropruina sp. (wastewater metagenome)
  ORGANISM  Micropruina sp.
            Bacteria; Bacillati; Actinomycetota; Actinomycetes;
            Propionibacteriales; Nocardioidaceae; Micropruina.
REFERENCE   1  (bases 1 to 2887)
  AUTHORS   Abdulkadir,N., Saraiva,J.P., Zhang,J., Stolte,S., Gillor,O.,
            Harms,H. and Rocha,U.
  TITLE     Genome-centric analyses of 165 metagenomes show that mobile genetic
            elements are crucial for the transmission of antimicrobial
            resistance genes to pathogens in activated sludge and wastewater
  JOURNAL   Microbiol Spectr 12 (3), e0291823 (2024)
   PUBMED   38289113
REFERENCE   2  (bases 1 to 2887)
  AUTHORS   Abdulkadir,N.
  TITLE     Direct Submission
  JOURNAL   Submitted (24-MAR-2023) Environmental Microbiology, Helmholtz
            Center for Environmental Research -UFZ, Permoserstr 15, Leipzig
            04318, Germany
COMMENT     The annotation was added by the NCBI Prokaryotic Genome Annotation
            Pipeline (PGAP). Information about PGAP can be found here:
            https://www.ncbi.nlm.nih.gov/genome/annotation_prok/
            
            ##Genome-Assembly-Data-START##
            Assembly Method        :: SPAdes v. 3.11.1
            Genome Representation  :: Full
            Expected Final Version :: Yes
            Genome Coverage        :: Not Applicable
            Sequencing Technology  :: Illumina MiSeq
            ##Genome-Assembly-Data-END##
            
            ##Genome-Annotation-Data-START##
            Annotation Provider               :: NCBI
            Annotation Date                   :: 05/04/2023 04:57:10
            Annotation Pipeline               :: NCBI Prokaryotic Genome
                                                 Annotation Pipeline (PGAP)
            Annotation Method                 :: Best-placed reference protein
                                                 set; GeneMarkS-2+
            Annotation Software revision      :: 6.5
            Features Annotated                :: Gene; CDS; rRNA; tRNA; ncRNA
            Genes (total)                     :: 2,776
            CDSs (total)                      :: 2,750
            Genes (coding)                    :: 2,714
            CDSs (with protein)               :: 2,714
            Genes (RNA)                       :: 26
            tRNAs                             :: 24
            ncRNAs                            :: 2
            Pseudo Genes (total)              :: 36
            CDSs (without protein)            :: 36
            Pseudo Genes (ambiguous residues) :: 0 of 36
            Pseudo Genes (frameshifted)       :: 2 of 36
            Pseudo Genes (incomplete)         :: 32 of 36
            Pseudo Genes (internal stop)      :: 2 of 36
            ##Genome-Annotation-Data-END##
FEATURES             Location/Qualifiers
     source          1..2887
                     /organism="Micropruina sp."
                     /mol_type="genomic DNA"
                     /submitter_seqid="NODE_13958_length_2887_cov_5.109816"
                     /isolate="AMR_MDS_2195"
                     /isolation_source="Activated sludge of municipal
                     wastewater treatment plants"
                     /db_xref="taxon:2737536"
                     /environmental_sample
                     /geo_loc_name="Canada"
                     /lat_lon="49.25 N 123.1 W"
                     /collection_date="2015-06-16"
                     /metagenome_source="wastewater metagenome"
                     /note="metagenomic"
     gene            complement(<1..696)
                     /locus_tag="PLF56_11920"
     CDS             complement(<1..696)
                     /locus_tag="PLF56_11920"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_011756963.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_function: GO:0016491 - oxidoreductase activity
                     [Evidence IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="aldehyde dehydrogenase family protein"
                     /protein_id="HOQ54304.1"
                     /translation="MSRTDVRKTYKLYIGGAFPRSESGRTYLVETADGTTANASKASR
                     KDARDAVGAARKAFGGWSGRTAYNRGQVVYRVAEVLESRRAQFEAELVGAGSAAAQAG
                     VEVDAAVDRLVWYAGWTDKIAQVHGSANPVAGPYFNHSAPEPAGVVAVVAPASPLLGL
                     VSVVAPLIAVGNTCVVIASQPAPLLAITFAEVLATSDVPGGVVNILTGDAAEIGPWLA
                     SHMDINGLDLTGVT"
     gene            complement(693..2123)
                     /locus_tag="PLF56_11925"
     CDS             complement(693..2123)
                     /locus_tag="PLF56_11925"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_011756962.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_function: GO:0016491 - oxidoreductase activity
                     [Evidence IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="aldehyde dehydrogenase family protein"
                     /protein_id="HOQ54305.1"
                     /translation="MSTTFEYAPAPESRAIVDIKPSYGLFIDGDFVDGHGEGFKTINP
                     ATEEVLAEISEATDADVDLAVHAARKAFRRWSRISGAERGKYLFRIARIIAERSRELA
                     VLESIDNGKPIKESRDVDVPTVAAHFFYYAGWADKLEHAGFGPNPKPLGVAGQVIPWN
                     FPLLMLAWKIAPALACGNTVVLKPAETTPLTALLFAEICQQADLPPGVVNIVTGAGGT
                     GRAVVAHPDVDKVAFTGSTEVGKAIARTVAGSHKKVTLELGGKAANIVFDDAPLDQAV
                     EGIVNGIFFNQGHVCCAGSRLLVQESVADEVLAKLKTRMATLRVGDPLDKNTDIGAIN
                     SAEQLAKIRALSDIGEAEGAGRWSPACELPERGFWFAPTIFTGVSQAHRIAREEIFGP
                     VLSVLTFRTPKEAVEKANNTPFGLSAGVWTEKGSRILWMGSQLRAGVVWSNTFNRFDP
                     ASPFGGYKESGYGREGGRHGLEGYLQ"
     gene            complement(2142..>2887)
                     /gene="deoC"
                     /locus_tag="PLF56_11930"
     CDS             complement(2142..>2887)
                     /gene="deoC"
                     /locus_tag="PLF56_11930"
                     /EC_number="4.1.2.4"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_011756961.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_function: GO:0004139 - deoxyribose-phosphate aldolase
                     activity [Evidence IEA];
                     GO_process: GO:0009264 - deoxyribonucleotide catabolic
                     process [Evidence IEA]"
                     /codon_start=3
                     /transl_table=11
                     /product="deoxyribose-phosphate aldolase"
                     /protein_id="HOQ54306.1"
                     /translation="MDTPGKVRALCSKAMRPDPADPSCPHTAAVCVYGDMVGIAKQAL
                     QGSPVHVASVATAFPSGRASRTVRLEDTAEAAAAGADEIDMVIDRGAFLAGRYLEVFD
                     DIVAVREACGSAHLKVIFETGELQTYDNVRRASWLAMLAGAHFIKTSTGKIQPAATLP
                     VTYLMVQAVRDFREATGQMVGVKPAGGIRSAKDAIKYLVMVNEVAGPDWLTPDWFRFG
                     ASTLLNDLLMQRTKMTTGRYSGPDYFTLD"
BASE COUNT          434 a          973 c         1010 g          470 t
ORIGIN      
        1 ggtcaccccg gtcaggtcga ggccgttgat gtccatgtgg gacgccagcc acggtccgat
       61 ctcggcggcg tccccggtca ggatgttcac caccccgccc ggcacgtcgg acgtggccag
      121 cacctcggcg aaggtgatgg ccagcagcgg ggccggctgc gacgcgatca ccacgcaggt
      181 gttgccgacc gcgatcagcg gcgccaccac cgacaccagc ccgagcaggg gggaggcggg
      241 cgcgaccacc gcgacgaccc cggccggctc aggtgctgag tggttgaagt agggtccggc
      301 tacgggattc gccgagccgt gcacctgggc gatcttgtcg gtccagcccg cgtaccagac
      361 caaccggtca acggcggcat ccacctcgac gccggcctgg gcggcagctg aaccggcccc
      421 aaccagctcg gcctcgaact gggcccgccg cgactccagc acctcggcga cgcggtagac
      481 cacctggccg cggttgtagg cggtgcggcc cgaccagccg ccgaacgcct tgcgggcggc
      541 acccaccgcg tcgcgggcgt cctttcggga ggccttggac gcgttggcgg tcgtgccgtc
      601 cgcggtctcg accaggtacg tgcggcccga ctccgagcgc gggaacgcgc cgccgatgta
      661 cagcttgtag gtcttgcgga cgtcggtgcg gctcactgca ggtacccttc cagtccgtgc
      721 cggccgcctt cgcggccgta gcccgactct ttgtagccgc cgaacgggct ggccgggtcg
      781 aaccggttga acgtgttgct ccacaccacg ccggcgcgca gttgcgagcc catccacaag
      841 atgcgggagc ccttctcggt ccataccccg gccgacaggc cgaacggggt gttgttcgcc
      901 ttctcgacgg cttccttcgg ggtgcggaag gtgagcaccg acagcaccgg cccgaagatc
      961 tcttcgcgag cgattcggtg ggcttgcgac accccggtga agatcgtcgg cgcgaaccag
     1021 aagccgcgct cgggcagctc gcaggcgggc gaccatcggc cggcgccctc ggcctcgccg
     1081 atgtctgaca acgcacgaat ctttgccaac tgctcggcgg agttgatcgc cccaatgtcg
     1141 gtgttcttgt cgagcgggtc acctacccgc agggtggcca tgcgggtctt caacttggcc
     1201 aacacctcgt cggctaccga ctcttgcacc agcagccgcg aaccggcgca gcagacgtgg
     1261 ccctggttga aaaagatgcc gttcacgatg ccctcgacgg cctggtcgag aggggcgtcg
     1321 tcgaacacga tgttggccgc cttgccgccc agttcgaggg tgaccttctt gtgactgccc
     1381 gcgacggtgc gggcgatcgc cttgcccacc tcggtcgaac cggtgaaggc caccttgtcg
     1441 acgtccgggt gggcgaccac ggcccgtccg gtgccccccg cgccggtgac gatgttcacc
     1501 acgccgggcg gcaggtcggc ctgctggcag atctcggcga agagcagggc ggtcagcggg
     1561 gtggtctcgg ccggcttcag caccacggtg ttgccgcagg ccagcgcggg ggcgatcttc
     1621 caggccagca tcagcagcgg aaagttccac ggaatcacct gcccggccac gcccagcggc
     1681 ttcgggttgg gcccgaaacc cgcgtgttcg agcttgtcgg cccagccggc gtagtagaag
     1741 aagtgcgcgg cgaccgtcgg cacgtcgacg tcgcgcgact ctttgatcgg cttgccgttg
     1801 tcgatcgact cgagcaccgc cagttcacgc gagcgctcgg cgatgatgcg ggcgatgcga
     1861 aacaggtact taccgcgctc ggcgccgctg atgcgcgacc acctgcgaaa ggccttgcgg
     1921 gcggcgtgca cggcgaggtc gacgtcggcg tcggtggcct ccgagatctc ggccagtacc
     1981 tcttcggtgg ccgggttgat ggtcttgaag ccctcgccgt gaccgtcgac gaagtcaccg
     2041 tcgatgaaca gcccgtagct gggcttgatg tcgacgatcg cgcgcgactc gggcgccggc
     2101 gcgtactcga acgtggtgct catgctgtgc cgttccagtt ctcagtcgag ggtgaagtag
     2161 tcggggccgg aataccgtcc ggtggtcatc ttggtgcgct gcatcagcag gtcgttgagc
     2221 agcgtcgagg cgccgaacct gaaccagtcg ggggtcagcc agtcggggcc ggccacctcg
     2281 ttgaccatca ccaggtactt gatggcgtcc ttggcgctgc gaatgccgcc ggcgggtttg
     2341 acgccgacca tctggcccgt cgcctcacga aaatcacgga cggcctgcac catcaggtag
     2401 gtgaccggca gggtggcggc gggctggatc ttgccggtgg aggtcttgat gaagtgcgcg
     2461 ccggcaagca tggccagcca ggacgcccgg cgcacgttgt cgtaggtctg cagctcaccg
     2521 gtttcgaaga tcaccttcag gtgcgcgctg ccgcacgcct cacgaacggc gacgatgtcg
     2581 tcgaacacct ccaggtagcg gccggcgaga aaggctccgc ggtcgatcac catgtcgatc
     2641 tcgtcggctc cggccgctgc ggcttcggca gtgtcttcga ggcggacggt gcgcgaggca
     2701 cgtccgctcg gaaacgccgt ggcaacggag gcgacgtgca cgggggagcc ctgcagggcc
     2761 tgcttggcga tgcccaccat gtcgccgtag acgcacacgg cggcggtgtg tgggcatgac
     2821 gggtcggccg ggtcgggacg catcgccttg ctgcacaggg cgcgcacctt gcccggggtg
     2881 tccatgc
//
LOCUS       DAOSUG010000410         2883 bp    DNA     linear   ENV 10-JUN-2024
DEFINITION  MAG TPA_asm: Micropruina sp. isolate AMR_MDS_2195
            NODE_13990_length_2883_cov_3.636846, whole genome shotgun sequence.
ACCESSION   DAOSUG010000410 DAOSUG010000000
VERSION     DAOSUG010000410.1
DBLINK      BioProject: PRJNA925477
            BioSample: SAMN32864262
            Sequence Read Archive: SRR4236673
KEYWORDS    WGS; ENV; Metagenome Assembled Genome; MAG; Third Party Data; TPA;
            TPA:assembly.
SOURCE      Micropruina sp. (wastewater metagenome)
  ORGANISM  Micropruina sp.
            Bacteria; Bacillati; Actinomycetota; Actinomycetes;
            Propionibacteriales; Nocardioidaceae; Micropruina.
REFERENCE   1  (bases 1 to 2883)
  AUTHORS   Abdulkadir,N., Saraiva,J.P., Zhang,J., Stolte,S., Gillor,O.,
            Harms,H. and Rocha,U.
  TITLE     Genome-centric analyses of 165 metagenomes show that mobile genetic
            elements are crucial for the transmission of antimicrobial
            resistance genes to pathogens in activated sludge and wastewater
  JOURNAL   Microbiol Spectr 12 (3), e0291823 (2024)
   PUBMED   38289113
REFERENCE   2  (bases 1 to 2883)
  AUTHORS   Abdulkadir,N.
  TITLE     Direct Submission
  JOURNAL   Submitted (24-MAR-2023) Environmental Microbiology, Helmholtz
            Center for Environmental Research -UFZ, Permoserstr 15, Leipzig
            04318, Germany
COMMENT     The annotation was added by the NCBI Prokaryotic Genome Annotation
            Pipeline (PGAP). Information about PGAP can be found here:
            https://www.ncbi.nlm.nih.gov/genome/annotation_prok/
            
            ##Genome-Assembly-Data-START##
            Assembly Method        :: SPAdes v. 3.11.1
            Genome Representation  :: Full
            Expected Final Version :: Yes
            Genome Coverage        :: Not Applicable
            Sequencing Technology  :: Illumina MiSeq
            ##Genome-Assembly-Data-END##
            
            ##Genome-Annotation-Data-START##
            Annotation Provider               :: NCBI
            Annotation Date                   :: 05/04/2023 04:57:10
            Annotation Pipeline               :: NCBI Prokaryotic Genome
                                                 Annotation Pipeline (PGAP)
            Annotation Method                 :: Best-placed reference protein
                                                 set; GeneMarkS-2+
            Annotation Software revision      :: 6.5
            Features Annotated                :: Gene; CDS; rRNA; tRNA; ncRNA
            Genes (total)                     :: 2,776
            CDSs (total)                      :: 2,750
            Genes (coding)                    :: 2,714
            CDSs (with protein)               :: 2,714
            Genes (RNA)                       :: 26
            tRNAs                             :: 24
            ncRNAs                            :: 2
            Pseudo Genes (total)              :: 36
            CDSs (without protein)            :: 36
            Pseudo Genes (ambiguous residues) :: 0 of 36
            Pseudo Genes (frameshifted)       :: 2 of 36
            Pseudo Genes (incomplete)         :: 32 of 36
            Pseudo Genes (internal stop)      :: 2 of 36
            ##Genome-Annotation-Data-END##
FEATURES             Location/Qualifiers
     source          1..2883
                     /organism="Micropruina sp."
                     /mol_type="genomic DNA"
                     /submitter_seqid="NODE_13990_length_2883_cov_3.636846"
                     /isolate="AMR_MDS_2195"
                     /isolation_source="Activated sludge of municipal
                     wastewater treatment plants"
                     /db_xref="taxon:2737536"
                     /environmental_sample
                     /geo_loc_name="Canada"
                     /lat_lon="49.25 N 123.1 W"
                     /collection_date="2015-06-16"
                     /metagenome_source="wastewater metagenome"
                     /note="metagenomic"
     gene            complement(<1..405)
                     /locus_tag="PLF56_11935"
     CDS             complement(<1..405)
                     /locus_tag="PLF56_11935"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_004808799.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="ABC transporter"
                     /protein_id="HOQ54307.1"
                     /translation="MSRPLPERLKALGEAAELSRGRASAAAVERADAVLKRAGERVAF
                     SGDHTVVALAGATGSGKSSSFNAISGTEFASVGVRRPTTSQAMGVAWGSELPGELLDW
                     LDVPRRHLVPARRSAFENLVLLDLPDHDSTEAE"
     gene            complement(402..2291)
                     /locus_tag="PLF56_11940"
     CDS             complement(402..2291)
                     /locus_tag="PLF56_11940"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_013864580.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_function: GO:0005525 - GTP binding [Evidence IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="50S ribosome-binding GTPase"
                     /protein_id="HOQ54308.1"
                     /translation="MSAGQQLAESLTSLRDALQQARLPLELPGGRLAGTLRHDMIAQL
                     DDYLLPRLQRLDAPLLAVIGGSTGAGKSTLVNSLIGRVVSRTGVIRPTTRSPVLVHHP
                     DDAHWFGPDRILPGLARSMEAANDTRTLQLVPEPSIPRGLALLDAPDIDSVVAENRAL
                     AAQLLAAADLWLFVTSAARYADAVPWDYLRSAAERSAALAVVLQRVPPAAMTEVPPHL
                     GQMMAERGLGDSPLFAVPETVTDNEGLLPDSAVAPIRDWLAALAADSQVRTAVVLKTL
                     DGAIGAVCRNAPTVAKAVDDQATAIEQLRNDAEGSYREAVRTVGVQTADGTLLRGEVL
                     SRWHDFVGTGEFFRAVEQKIGWLRDRLMASLRGEPKQVNDVKLAVESGMEVLIREEGE
                     AAAERAETAWANDPAGRELLAAARSDLGRASDGFPAAVSLAIRNWQADVLELVSNEGA
                     SKKSKARFFALGANGVGVALMMVVFSMTGGLTGAELGVAGGTAVLAQRVLEAVFGEDA
                     VRRLAKVAKDELDARLEALMASELTRYETVLDDAGVRPDSAEAIRAAVQQVNEVRGGD
                     ERLTPPTPSELEGVRGTATQVQHRPQAIDEVAVAELLPGGSGPGDGAVATGPGSVVEV
                     DGEPS"
     gene            complement(2301..>2883)
                     /locus_tag="PLF56_11945"
     CDS             complement(2301..>2883)
                     /locus_tag="PLF56_11945"
                     /inference="COORDINATES: ab initio
                     prediction:GeneMarkS-2+"
                     /note="Derived by automated computational analysis using
                     gene prediction method: GeneMarkS-2+."
                     /codon_start=2
                     /transl_table=11
                     /product="hypothetical protein"
                     /protein_id="HOQ54309.1"
                     /translation="SQATFASGPMRYLIYFAVALPLVASAVIYVARQLVKESQKIRAR
                     LTDYVRMGWLSPVDPETTSRVRRRAWALLVALTRGWAVFRSTLSLQRTLTELAYLRDA
                     EVKGVIDNAGQQRAKELLYHARSLRSLAIDDPSGQRLSLPRLRKPKPVDYAPPQYPGP
                     AGLGGNWPATPGSAPLGSQTYSRVDPTWGPPPA"
BASE COUNT          421 a         1072 c          975 g          415 t
ORIGIN      
        1 ctcggcctcg gtgctgtcgt gatcgggcag gtcgagcagc accagattct cgaacgcgct
       61 gcgccgggcg ggcaccagat gccggcgcgg cacatcgagc cagtccagca gttcacctgg
      121 cagctcgctg ccccaggcca cgcccatggc ctgtgaggtg gtcggacggc gcacgcccac
      181 cgatgcgaac tcggtgccgc tgatcgcgtt gaacgacgac gacttgcccg aaccggtcgc
      241 acccgcgagt gccacgacgg tgtggtcacc agaaaaggcc acccgttcgc ccgcccgctt
      301 cagcacggca tcggcccgtt cgaccgcggc ggcggacgcc cgtccgcggc tcagttcggc
      361 ggcttcaccg agcgccttca accgctccgg caatgggcgg ctcacgacgg ctctccgtcg
      421 acctcgacga ccgacccggg gccggtcgcg acggcaccgt cacctggccc cgaccccccc
      481 ggaagcaact ctgccaccgc gacctcgtcg atcgcctgcg gacggtgctg cacctgggtc
      541 gccgtgcccc ggacgccttc caactctgag ggggtgggtg gcgtcaatcg ctcatcacca
      601 ccccgaacct cgttgacctg ctgcaccgct gctcgaatcg cctcggcgct gtcgggccgc
      661 accccggcat cgtccagcac cgtctcgtaa cgagtcagtt ccgaggccat cagcgcctcc
      721 agccgggcgt ccagctcgtc ttttgccacc ttcgcgagcc gccgcacggc gtcttcgccg
      781 aacaccgcct caagcacccg ctgggccagc accgcagtgc cgcccgccac cccgagttcg
      841 gcaccggtca ggccgccggt catcgagaac accaccatca tcagcgcgac gccgacaccg
      901 ttggcgccga gcgcgaagaa ccgtgccttc gacttcttgg acgccccctc gttgctgacc
      961 agttcgagca cgtccgcctg ccagttgcgg atcgccagcg acacggccgc ggggaaaccg
     1021 tccgaggcgc gccccagatc ggagcgtgcg gccgcgagca actcccgtcc ggccgggtcg
     1081 ttggcccagg ccgtctcggc ccgctcggcg gccgcctcgc cctcttcgcg gatcagcacc
     1141 tccatgcccg actcgaccgc cagcttcacg tcgttgacct gcttgggctc gccgcgcagc
     1201 gaggccatca gccggtcgcg cagccagccg atcttctgct ccaccgcacg gaagaactcg
     1261 ccggtgccga cgaagtcgtg ccagcgcgac agcacctcgc cgcgcagcag ggtgccgtcg
     1321 gcggtctgca cccccaccgt gcggacggcc tcgcggtacg acccctccgc gtcgttgcgc
     1381 aactgctcga tcgccgtggc ctggtcgtcg accgctttcg ccactgtggg cgcgttgcga
     1441 caaaccgccc cgatcgcacc gtccagggtc ttgagcacca ccgccgtgcg cacctggctg
     1501 tcggccgcca gcgcggccag ccagtcgcgg atcggcgcga ccgccgagtc gggcagcaac
     1561 ccctcgttgt cggtgacggt ctcgggcacg gcgaacagcg gcgagtcgcc cagtccgcgc
     1621 tcggccatca tctgacccag gtgcggcggc acctcggtca tcgccgccgg cgggacgcgc
     1681 tgcagcacga ccgccaacgc ggcgctgcgc tcggcggccg agcgcagata gtcccacggc
     1741 acggcgtcgg cgtagcgcgc cgccgaggtg acgaagagcc acaggtcggc cgccgccagc
     1801 agttgcgcgg ccagcgcccg gttctcggcc accaccgagt cgatgtcggg tgcgtccagc
     1861 agcgccaggc cccgcggaat ggacggctcc ggcaccagtt gcagggttcg cgtgtcgttg
     1921 gcggcctcca tcgagcgggc cagcccgggc agaatgcggt cggggccgaa ccagtgcgcg
     1981 tcgtccggat gatgcaccag caccggcgat cgggtggtcg gacggatcac gccggtgcgg
     2041 ctcaccaccc gtccgatcag ggagttgacc agggtggact tgcccgcgcc ggtcgagccg
     2101 ccgatcaccg ccagcagcgg agcgtccagg cgttgcagcc ggggcagcag gtagtcatcg
     2161 agctgggcga tcatgtcgtg ccgcagggtg ccggccagcc ggccgccggg cagttccagc
     2221 ggtagccggg cctgctgcag ggcatcacgc aacgaggtga gcgactccgc cagttgttgg
     2281 ccagcgctca tcgactctcc ttatgcgggg ggtgggcccc acgtgggatc cactcgtgaa
     2341 tacgtctgcg atcctaacgg tgccgagccc ggcgtggcgg gccagttgcc gccgagtcct
     2401 gccgggcctg gatactgcgg cggagcgtaa tcgacgggct tcggcttgcg caggcgcggc
     2461 aggctgaggc gttggccgga cgggtcgtcg atcgcgagac tccgcaacga ccgggcgtgg
     2521 tacagcagtt ccttggcccg ctgctggccg gcgttgtcga tcacgccctt gacctcggcg
     2581 tcgcgcagat aggccagttc ggtcagggtg cgttgcagac tgagcgtcga gcggaacacc
     2641 gcccagccgc gggtgagcgc gaccagcagg gcccaggccc ggcggcgcac ccgcgaggtg
     2701 gtctcgggat cgaccgggct gagccaaccc atgcgcacgt aatcggtcag ccgggctcga
     2761 atcttctggc tctctttgac cagttgacgc gccacgtaga tcaccgccga ggcaaccagg
     2821 ggcagcgcta cggcgaagta gatcaggtag cgcatcgggc cggaggcgaa cgtggcctgg
     2881 ctg
//
LOCUS       DAOSUG010000411         2881 bp    DNA     linear   ENV 10-JUN-2024
DEFINITION  MAG TPA_asm: Micropruina sp. isolate AMR_MDS_2195
            NODE_14003_length_2881_cov_4.001062, whole genome shotgun sequence.
ACCESSION   DAOSUG010000411 DAOSUG010000000
VERSION     DAOSUG010000411.1
DBLINK      BioProject: PRJNA925477
            BioSample: SAMN32864262
            Sequence Read Archive: SRR4236673
KEYWORDS    WGS; ENV; Metagenome Assembled Genome; MAG; Third Party Data; TPA;
            TPA:assembly.
SOURCE      Micropruina sp. (wastewater metagenome)
  ORGANISM  Micropruina sp.
            Bacteria; Bacillati; Actinomycetota; Actinomycetes;
            Propionibacteriales; Nocardioidaceae; Micropruina.
REFERENCE   1  (bases 1 to 2881)
  AUTHORS   Abdulkadir,N., Saraiva,J.P., Zhang,J., Stolte,S., Gillor,O.,
            Harms,H. and Rocha,U.
  TITLE     Genome-centric analyses of 165 metagenomes show that mobile genetic
            elements are crucial for the transmission of antimicrobial
            resistance genes to pathogens in activated sludge and wastewater
  JOURNAL   Microbiol Spectr 12 (3), e0291823 (2024)
   PUBMED   38289113
REFERENCE   2  (bases 1 to 2881)
  AUTHORS   Abdulkadir,N.
  TITLE     Direct Submission
  JOURNAL   Submitted (24-MAR-2023) Environmental Microbiology, Helmholtz
            Center for Environmental Research -UFZ, Permoserstr 15, Leipzig
            04318, Germany
COMMENT     The annotation was added by the NCBI Prokaryotic Genome Annotation
            Pipeline (PGAP). Information about PGAP can be found here:
            https://www.ncbi.nlm.nih.gov/genome/annotation_prok/
            
            ##Genome-Assembly-Data-START##
            Assembly Method        :: SPAdes v. 3.11.1
            Genome Representation  :: Full
            Expected Final Version :: Yes
            Genome Coverage        :: Not Applicable
            Sequencing Technology  :: Illumina MiSeq
            ##Genome-Assembly-Data-END##
            
            ##Genome-Annotation-Data-START##
            Annotation Provider               :: NCBI
            Annotation Date                   :: 05/04/2023 04:57:10
            Annotation Pipeline               :: NCBI Prokaryotic Genome
                                                 Annotation Pipeline (PGAP)
            Annotation Method                 :: Best-placed reference protein
                                                 set; GeneMarkS-2+
            Annotation Software revision      :: 6.5
            Features Annotated                :: Gene; CDS; rRNA; tRNA; ncRNA
            Genes (total)                     :: 2,776
            CDSs (total)                      :: 2,750
            Genes (coding)                    :: 2,714
            CDSs (with protein)               :: 2,714
            Genes (RNA)                       :: 26
            tRNAs                             :: 24
            ncRNAs                            :: 2
            Pseudo Genes (total)              :: 36
            CDSs (without protein)            :: 36
            Pseudo Genes (ambiguous residues) :: 0 of 36
            Pseudo Genes (frameshifted)       :: 2 of 36
            Pseudo Genes (incomplete)         :: 32 of 36
            Pseudo Genes (internal stop)      :: 2 of 36
            ##Genome-Annotation-Data-END##
FEATURES             Location/Qualifiers
     source          1..2881
                     /organism="Micropruina sp."
                     /mol_type="genomic DNA"
                     /submitter_seqid="NODE_14003_length_2881_cov_4.001062"
                     /isolate="AMR_MDS_2195"
                     /isolation_source="Activated sludge of municipal
                     wastewater treatment plants"
                     /db_xref="taxon:2737536"
                     /environmental_sample
                     /geo_loc_name="Canada"
                     /lat_lon="49.25 N 123.1 W"
                     /collection_date="2015-06-16"
                     /metagenome_source="wastewater metagenome"
                     /note="metagenomic"
     gene            complement(<1..509)
                     /locus_tag="PLF56_11950"
     CDS             complement(<1..509)
                     /locus_tag="PLF56_11950"
                     /inference="COORDINATES: protein motif:HMM:NF014766.2"
                     /note="starvation-sensing protein; maybe involved in
                     homoserine lactone degradation; Derived by automated
                     computational analysis using gene prediction method:
                     Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="bifunctional D-altronate/D-mannonate
                     dehydratase"
                     /protein_id="HOQ54310.1"
                     /translation="MTDIIEKVEVLVSSPSRNYVTLRITTRDGIVGLGDATVNGRELS
                     VAAYLRDHVGPLLIGRDPGRIEDTWQYLYRGVYWRRGPIGMAAIGAVDIALWDILGKR
                     TGQPLYQLFGGRVRNRLATYRHAFGWDLPALLQQVDAHREAGFTNIRVQTGVPELDVV
                     YGVTKDGPV"
     gene            complement(514..1620)
                     /locus_tag="PLF56_11955"
     CDS             complement(514..1620)
                     /locus_tag="PLF56_11955"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_015011366.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="sugar kinase"
                     /protein_id="HOQ54311.1"
                     /translation="MTNQLTLRPADECRYDALALGEVMLRLDPGELRIRTARSFTAWE
                     GGGEYNVVRGLSRVFDQRAGVITALVDDEVGHLIEGMIMAGGVDTSLIEWAASDGVGR
                     TVRNGLNFTERGFGVRGAKGVNDRGHTAIAQLGPDAIDFDHIFGTLGVRWLHTGGIYA
                     ALSETSAATALAAIKSAKKHGTIVSYDLNYRPSLWKSIGGQARAQEVNRELAQYVDVM
                     IGNEEDFTASLGFEIEGVDENLTELPIEGFQRMIETAAAAYPNFQVIATTLRAVHSAS
                     DNDWSALAWSPSKGLVQAADRKHLEILDRVGGGDSFASGLCYGLISGEDLQTAVEYGA
                     AHGALAMTTPGDTTMATKAEVLKLAGGGSARVDR"
     gene            complement(1650..2279)
                     /gene="eda"
                     /locus_tag="PLF56_11960"
     CDS             complement(1650..2279)
                     /gene="eda"
                     /locus_tag="PLF56_11960"
                     /EC_number="4.1.2.14"
                     /EC_number="4.1.3.16"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_002531183.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_function: GO:0008675 -
                     2-dehydro-3-deoxy-phosphogluconate aldolase activity
                     [Evidence IEA];
                     GO_function: GO:0008700 - 4-hydroxy-2-oxoglutarate
                     aldolase activity [Evidence IEA];
                     GO_process: GO:0009084 - glutamine family amino acid
                     biosynthetic process [Evidence IEA];
                     GO_process: GO:0009255 - Entner-Doudoroff pathway through
                     6-phosphogluconate [Evidence IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="bifunctional 4-hydroxy-2-oxoglutarate
                     aldolase/2-dehydro-3-deoxy-phosphogluconate aldolase"
                     /protein_id="HOQ54312.1"
                     /translation="MSELINDVLAARIVPVVVVRDASRAAGLAEALVAGNLPVAEVTL
                     RTPAAAEAIATMAQNPDLLVGAGTVITPAQVDLAVDAGARFLVSPGFSAETVARAAER
                     GVPIVPGTVTPSEVIAAMAAGLSLLKFFPASNYGGAGTLKAFSSVFSGIDFIPTGGVS
                     ASNIGDYLSLPNVPAVGGSWMVPADVVDAGDFATIESLSAEAVAAAAAI"
     gene            2452..>2881
                     /locus_tag="PLF56_11965"
     CDS             2452..>2881
                     /locus_tag="PLF56_11965"
                     /inference="COORDINATES: protein motif:HMM:NF020897.2"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_function: GO:0003677 - DNA binding [Evidence IEA];
                     GO_process: GO:0006355 - regulation of DNA-templated
                     transcription [Evidence IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="helix-turn-helix domain-containing protein"
                     /protein_id="HOQ54313.1"
                     /translation="MTDGPRVEAIDRALNLLTTLAEAGAGGLPLTELAASTGVNKSTA
                     YRALSTMRAHGFVSQSEATGDYRLGAVAMSLGERFVTPQSLAQSLHPALVALSREAEE
                     LVHLGVLIGDQVLYIDKVEPERAIRVWSEVGRRTSAASSAM"
BASE COUNT          432 a          949 c         1028 g          472 t
ORIGIN      
        1 tagaccgggc cgtccttggt gaccccgtac accacgtcga gttcgggcac gccggtctgc
       61 acccggatgt tggtgaagcc ggcctcgcgg tgggcgtcca cctgctgcag cagggcgggc
      121 aggtcccagc cgaaggcgtg ccggtaggtg gccagccggt tgcggacccg tccgccgaac
      181 agctggtaca ggggctggcc ggtgcgcttg ccgagaatgt cccacagcgc gatgtcgacc
      241 gcaccgatgg cggccatgcc gatcgggccg cggcgccagt agacgccgcg gtagaggtac
      301 tgccaggtgt cttcgatgcg gcccgggtcg cgtccgatca gcaacggacc gacatggtcg
      361 cgcaggtagg cggcgaccga gagttcgcgt ccgttgacgg tggcgtcgcc gaggccgacg
      421 atgccgtcgc gggtggtgat ccgcagcgtc acgtagttgc gggacgggct gctgaccagc
      481 acctcgacct tctcgatgat gtcggtcatc gggtcaccgg tccacccggg cgctgccgcc
      541 gccggccagc ttgagcactt cggccttcgt ggccatcgtg gtgtcgccgg gggtggtcat
      601 cgccagggcg ccgtgggcgg cgccgtactc gaccgcggtc tgcaggtctt cgccgctgat
      661 caggccgtag cagaggccgg acgcgaacga gtcgccgccg ccgacccggt cgagaatctc
      721 gaggtgcttg cggtcggcgg cctgcaccag gcctttgctc ggcgaccagg ccagcgcgct
      781 ccagtcgttg tcggacgcgc tgtgcacggc ccgcagcgta gtggcgatca cctggaagtt
      841 ggggtaggcc gccgcggcgg tctcgatcat gcgctggaag ccctcgatcg gcagctcggt
      901 caggttctcg tccacgcctt cgatctcgaa tccgagggac gcggtgaagt cctcctcgtt
      961 gccgatcatc acgtcgacgt actgcgcgag ctcgcggttc acctcttggg cgcgggcctg
     1021 gccgccgatg ctcttccaca ggctgggccg gtagttgagg tcgtagctga cgatggtgcc
     1081 gtgcttcttg gccgacttga tcgcggccag cgcggtggcg gcgctggtct cggacagcgc
     1141 ggcgtagatg ccgccggtgt gcagccagcg cacgcccaag gtgccgaaga tgtggtcgaa
     1201 gtcgatcgcg tcggggccga gttgggcgat cgcggtgtgg ccgcggtcgt tgacgccctt
     1261 ggcaccgcgg acgccgaagc cgcgctcggt gaagttgagc ccgttgcgga cggtgcgtcc
     1321 cacgccgtcg gacgctgccc actcgatcag cgaggtgtcg actcccccgg ccatgatcat
     1381 gccttcgatc aggtggccga cctcgtcgtc gaccagggcg gtgatcaccc cggcccgctg
     1441 gtcgaacacc cggctcagac cccggacgac gttgtactcg ccgccgccct cccaggcggt
     1501 gaagctgcgc gcggtgcgga tgcgcagctc accgggatcg agccgcagca tcacctcacc
     1561 cagggcgagg gcgtcgtagc ggcactcgtc cgcaggccgc agggtcagct ggttggtcat
     1621 ggcgggtgtc ctccggtggt tggtacgggt cagatggcgg cagcggcggc gaccgcttcg
     1681 gcgctgaggg attcgatggt ggcgaagtcg ccggcgtcca ccacgtcggc gggcaccatc
     1741 cacgagccgc cgaccgcggg cacgttcggc agactgaggt agtcgccgat attgctcgcc
     1801 gacacgccgc cggtgggaat gaagtcgatg cccgagaaca ccgacgagaa cgccttcagg
     1861 gtgccggcgc cgccgtagtt cgaggcggga aagaacttga gcaggctgag ccccgcggcc
     1921 atggcagcga tcacctcgct gggggtgacc gtgcccggca cgatcggcac gccgcgctcg
     1981 gcagcccgag cgacggtttc ggcgctgaac ccggggctga ccaggaacct cgccccggcg
     2041 tccacagcca ggtcgacctg cgcgggggtt atcacggtgc cggcgccgac cagcaggtcg
     2101 gggttctggg ccatcgtggc gatcgcctcg gcggcggcgg gggtacgcag cgtcacctcg
     2161 gcgacgggca ggttgccggc gacgagcgcc tcggccaggc cggcggcgcg ggaggcgtcg
     2221 cgcaccacga cgacgggcac gatccgggcg gccagcacat cgttgatcag ttcagacatc
     2281 tcacatctct ctcggttcaa ccactgcgtc gtcactatcg tttcaatcat tgaaacgttc
     2341 gagttcgttg tttgaaacat cttgacatga gagcgacctg agatgcaagc ctcttttcac
     2401 acactgaacc taaccgttgc gttgagtgaa acgatcgggc agtatcgagt aatgactgac
     2461 ggacctcgag tcgaggcgat tgatcgcgca ctcaatctgc tgacgaccct cgcggaggcc
     2521 ggcgcgggcg gacttccgct gaccgaactc gccgccagca ccggggtcaa caagtccacc
     2581 gcctaccggg cgctgtcgac catgcgcgcc cacggcttcg tcagtcagtc agaggccacc
     2641 ggcgactacc ggctgggcgc cgtggcgatg tcactgggcg aacgcttcgt caccccgcag
     2701 agcctggccc agtcgttgca tccggcgctg gtggccctgt cacgcgaagc ggaagagctg
     2761 gtgcacctgg gcgtgctgat cggcgaccag gtgctctata tcgacaaggt cgagcccgag
     2821 cgggcgatcc gggtgtggag cgaagtgggc cgccgaacct cggcggcgtc ctcagccatg
     2881 g
//
LOCUS       DAOSUG010000412         2879 bp    DNA     linear   ENV 10-JUN-2024
DEFINITION  MAG TPA_asm: Micropruina sp. isolate AMR_MDS_2195
            NODE_14016_length_2879_cov_4.121459, whole genome shotgun sequence.
ACCESSION   DAOSUG010000412 DAOSUG010000000
VERSION     DAOSUG010000412.1
DBLINK      BioProject: PRJNA925477
            BioSample: SAMN32864262
            Sequence Read Archive: SRR4236673
KEYWORDS    WGS; ENV; Metagenome Assembled Genome; MAG; Third Party Data; TPA;
            TPA:assembly.
SOURCE      Micropruina sp. (wastewater metagenome)
  ORGANISM  Micropruina sp.
            Bacteria; Bacillati; Actinomycetota; Actinomycetes;
            Propionibacteriales; Nocardioidaceae; Micropruina.
REFERENCE   1  (bases 1 to 2879)
  AUTHORS   Abdulkadir,N., Saraiva,J.P., Zhang,J., Stolte,S., Gillor,O.,
            Harms,H. and Rocha,U.
  TITLE     Genome-centric analyses of 165 metagenomes show that mobile genetic
            elements are crucial for the transmission of antimicrobial
            resistance genes to pathogens in activated sludge and wastewater
  JOURNAL   Microbiol Spectr 12 (3), e0291823 (2024)
   PUBMED   38289113
REFERENCE   2  (bases 1 to 2879)
  AUTHORS   Abdulkadir,N.
  TITLE     Direct Submission
  JOURNAL   Submitted (24-MAR-2023) Environmental Microbiology, Helmholtz
            Center for Environmental Research -UFZ, Permoserstr 15, Leipzig
            04318, Germany
COMMENT     The annotation was added by the NCBI Prokaryotic Genome Annotation
            Pipeline (PGAP). Information about PGAP can be found here:
            https://www.ncbi.nlm.nih.gov/genome/annotation_prok/
            
            ##Genome-Assembly-Data-START##
            Assembly Method        :: SPAdes v. 3.11.1
            Genome Representation  :: Full
            Expected Final Version :: Yes
            Genome Coverage        :: Not Applicable
            Sequencing Technology  :: Illumina MiSeq
            ##Genome-Assembly-Data-END##
            
            ##Genome-Annotation-Data-START##
            Annotation Provider               :: NCBI
            Annotation Date                   :: 05/04/2023 04:57:10
            Annotation Pipeline               :: NCBI Prokaryotic Genome
                                                 Annotation Pipeline (PGAP)
            Annotation Method                 :: Best-placed reference protein
                                                 set; GeneMarkS-2+
            Annotation Software revision      :: 6.5
            Features Annotated                :: Gene; CDS; rRNA; tRNA; ncRNA
            Genes (total)                     :: 2,776
            CDSs (total)                      :: 2,750
            Genes (coding)                    :: 2,714
            CDSs (with protein)               :: 2,714
            Genes (RNA)                       :: 26
            tRNAs                             :: 24
            ncRNAs                            :: 2
            Pseudo Genes (total)              :: 36
            CDSs (without protein)            :: 36
            Pseudo Genes (ambiguous residues) :: 0 of 36
            Pseudo Genes (frameshifted)       :: 2 of 36
            Pseudo Genes (incomplete)         :: 32 of 36
            Pseudo Genes (internal stop)      :: 2 of 36
            ##Genome-Annotation-Data-END##
FEATURES             Location/Qualifiers
     source          1..2879
                     /organism="Micropruina sp."
                     /mol_type="genomic DNA"
                     /submitter_seqid="NODE_14016_length_2879_cov_4.121459"
                     /isolate="AMR_MDS_2195"
                     /isolation_source="Activated sludge of municipal
                     wastewater treatment plants"
                     /db_xref="taxon:2737536"
                     /environmental_sample
                     /geo_loc_name="Canada"
                     /lat_lon="49.25 N 123.1 W"
                     /collection_date="2015-06-16"
                     /metagenome_source="wastewater metagenome"
                     /note="metagenomic"
     gene            complement(<1..503)
                     /locus_tag="PLF56_11970"
     CDS             complement(<1..503)
                     /locus_tag="PLF56_11970"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_013865523.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_function: GO:0008184 - glycogen phosphorylase activity
                     [Evidence IEA];
                     GO_process: GO:0005975 - carbohydrate metabolic process
                     [Evidence IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="glycogen/starch/alpha-glucan phosphorylase"
                     /protein_id="HOQ54314.1"
                     /translation="MSVPIAPKSAASPSQPADETPAVGHTHRGVQFPQTHPLAVAPVT
                     APPNTVDGFVREFLHELNTDLGVALSRSSINDQYTALAHTVRNYLTARWLETMRRNRE
                     LQPKTVAYLSAEYLLGRHLGNNLLAANLNDIAAKALNGCGLSLDTLRAQEGEPGLGNG
                     GLGRLAA"
     gene            663..1880
                     /locus_tag="PLF56_11975"
     CDS             663..1880
                     /locus_tag="PLF56_11975"
                     /EC_number="1.1.5.-"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_013865174.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_function: GO:0016901 - oxidoreductase activity, acting
                     on the CH-OH group of donors, quinone or similar compound
                     as acceptor [Evidence IEA];
                     GO_function: GO:0070968 - pyrroloquinoline quinone binding
                     [Evidence IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="PQQ-dependent sugar dehydrogenase"
                     /protein_id="HOQ54315.1"
                     /translation="MVSRLATSISALVALVLTACTASPAVDSAQSSGTSSAQSSGASS
                     PSQSAALSLPAASPMPGTPFAVQPLGAFDEPWALAFLPGGFDALVTERRGTLVLRHAD
                     GRTVDVEGVPTVAYGGQGGLGDVIVSPTFATDRTVYLSWAASGEGGTGAEVGRGRLVG
                     DGTSARLENFTVIWRQQPKVDGSGHFGHRLAFSPDGEYLFVTSGERQKMSPAQDLTNN
                     LGSIVRLNPDGTPAAGNPFADRGGVAAEIWTYGHRNPLGLAFDASGNLWSSEMGPQGG
                     DELNLVVEGRNYGWPQASNGSHYGGAEIPDHREGDGFEAPKVWWNPSISPGSLMIYSG
                     TRFPAWRGDAFLPALSGQGLARVDLQGTTARKADEWNFGARVRAVGQAPDGSLWVLED
                     GGNGRLLRLTRAG"
     gene            1950..2180
                     /locus_tag="PLF56_11980"
     CDS             1950..2180
                     /locus_tag="PLF56_11980"
                     /inference="COORDINATES: ab initio
                     prediction:GeneMarkS-2+"
                     /note="Derived by automated computational analysis using
                     gene prediction method: GeneMarkS-2+."
                     /codon_start=1
                     /transl_table=11
                     /product="hypothetical protein"
                     /protein_id="HOQ54316.1"
                     /translation="MPAPPTRMPVRAVLAPAARLDEATRKRLARFGLTLPAADVSDAG
                     EGIADVADRGDHNTRWRRDGVGFLTPAMAAPR"
     gene            complement(2279..>2879)
                     /locus_tag="PLF56_11985"
     CDS             complement(2279..>2879)
                     /locus_tag="PLF56_11985"
                     /inference="COORDINATES: protein motif:HMM:NF013895.2"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_function: GO:0008324 - monoatomic cation transmembrane
                     transporter activity [Evidence IEA];
                     GO_process: GO:0006812 - monoatomic cation transport
                     [Evidence IEA]"
                     /codon_start=2
                     /transl_table=11
                     /product="magnesium transporter"
                     /protein_id="HOQ54317.1"
                     /translation="ILEDAAHEDVARQGAAEPLAKPYLTTPVLGLVKSRAVWLLVLAV
                     SALLTVQVLGVFEETLEEVVTLALFIPLLTGTAGNAGAQAATTVTRALALGEVSARDV
                     LAVAWRERRVGLLLGGLLGALGFVLASLVFDTTFGAVIGLTLVAVCAIAATVGGAMPM
                     LARALHADPAVFSTPFISTFCDATSLIIYFGIATQLLHL"
BASE COUNT          430 a          991 c         1045 g          413 t
ORIGIN      
        1 caggctgcca accgaccgag gccaccgttg ccgaggccgg gctcgccctc ttgggcgcgc
       61 agggtgtcta ggctgaggcc gcagccgttg agggccttcg cggcgatgtc attgaggttg
      121 gcggccagca ggttgttgcc gagatgccgg ccgagcaggt actcggcaga caggtaggcg
      181 accgtcttgg gctgcaactc gcggttgcgg cgcatcgtct cgagccaacg tgcggtcaga
      241 tagttgcgca ccgtgtgcgc cagcgcagtg tattgatcgt tgatcgacga acgcgacagg
      301 gccacaccga ggtcggtgtt gagttcgtgc aggaactccc tcacgaagcc gtccaccgtg
      361 ttgggtgggg cggtcaccgg cgccaccgcg agcgggtggg tctgcggaaa ttggacgccg
      421 cggtgcgtat gaccgacggc aggggtttcg tctgccggct gggacggcga ggcagcggac
      481 ttcggcgcga taggcacaga catgcccaat accgtagtgg gcgattgtta cgggtagcac
      541 attcggacag cgggcagaca gcgtgccagg cccagcgagt tcttccgtgg ccgccacgag
      601 gcgagggcgc agcctgccgc cgggtacgta gcggattggg gtcgaccggc tattttcggt
      661 gaatggtaag cagactggcc acctcgatca gtgctttggt cgcgctcgtc ctgaccgcct
      721 gcaccgcctc gccggccgtg gactcggccc agtcgtccgg cacctcctcg gcccagtcgt
      781 ccggcgcatc ctcccccagc caatccgcgg cgctctcgtt gccggccgcg tccccgatgc
      841 ccggcacccc gttcgcggtg cagccgctgg gtgccttcga cgagccctgg gcgctggcgt
      901 tcctgcccgg cggtttcgac gcgctggtca ccgagcgccg cggcacgctg gtgctgcggc
      961 acgcggacgg gcgcacggtt gacgtcgagg gggtgccgac ggtggcctac ggcggccagg
     1021 gcgggttggg cgacgtgatc gtgtcgccca cgttcgccac cgaccgcacg gtgtacctca
     1081 gctgggcggc gtccggcgag ggcggcaccg gcgccgaggt gggccggggc aggttggtcg
     1141 gcgacggcac gtccgcgcgg ttggagaact tcacggtgat ctggcggcaa cagccgaagg
     1201 tggacgggtc cggccacttc gggcaccggc tggcgttctc gcccgacggt gagtacctgt
     1261 tcgtcacctc gggcgagcgg cagaagatga gcccggccca ggacctgacg aacaacctgg
     1321 gcagcatcgt ccggctcaat ccggacggca cgccggccgc cggcaacccc tttgccgacc
     1381 gtggcggagt ggccgccgag atatggacct acgggcaccg caatccgctc ggtctggcct
     1441 tcgacgcctc cggcaacctg tggagcagcg agatggggcc gcagggcggc gatgagctga
     1501 acctcgtcgt cgagggacgc aactacgggt ggccgcaggc gtccaacggg tcgcactacg
     1561 gaggtgccga gatccccgac caccgcgagg gcgacggctt cgaggcaccc aaggtgtggt
     1621 ggaacccgag catctcgccc ggcagcctga tgatctactc cgggacgcgc ttccccgcgt
     1681 ggcgcggcga cgccttcctg cccgcgctct ccggccaggg cctggcgcgc gtcgacctgc
     1741 agggcaccac cgcccgcaag gcggacgagt ggaacttcgg tgcccgcgtc cgcgccgtcg
     1801 gccaggcccc cgacggctcg ctctgggtgc tcgaggacgg tggtaacgga cggctgctgc
     1861 ggctcacccg ggccggctga gccggtcacc cgcccgcgga cggcccgcgc tgctcgcctc
     1921 actcgcagca agcatgaagg agccgccgga tgcccgcccc gccgacgcga atgcccgtcc
     1981 gcgcggttct cgcacccgcc gcgcgcctgg acgaggccac gcgcaagcga ctcgcccggt
     2041 ttggcctgac gctgccggcg gccgatgtat ccgatgcagg tgaggggatc gccgacgtcg
     2101 cggaccgcgg cgatcacaac actcgctggc ggcgcgacgg cgtcgggttt ctgaccccgg
     2161 ccatggcggc ccccaggtga gctccaccgc tggtgggcgc gacgcgagtg acgctccact
     2221 acggacgata gtggtcaagg tgccgccgcg tggacgtctt tccccgcgtg cggagtattc
     2281 acaagtgcag cagctgggtg gcgatcccga agtagatgat caggctggtc gcgtcgcaga
     2341 aggtggagat gaacggcgtc gagaacaccg ccggatcggc gtgcagggcc cgggccagca
     2401 tcggcatggc gccgcccacg gtcgccgcta tcgcacacac cgcgaccaga gtcagcccga
     2461 tcacggcgcc gaaggtggtg tcgaagacca gcgaggcgag cacgaagccc agcgcgccga
     2521 gcaggccgcc cagcagcaac ccgacccgtc tctcgcgcca ggccaccgcg agcacgtcgc
     2581 gggcactcac ctcgcccagc gccagggcgc gagtgaccgt ggtggcggcc tgagcgccgg
     2641 cgtttccggc cgtgccggtc agcagcggaa tgaacagcgc gagcgtcacg acctcctcca
     2701 gggtctcttc gaatacgccg agcacctgca cggtgagcag cgccgagacg gccagcacca
     2761 gcaaccagac cgctcgcgac ttgaccaggc ccagcacggg cgtggtcagg tacggcttgg
     2821 cgaggggctc ggccgcgccc tggcgggcaa cgtcctcgtg ggctgcgtcc tcaaggatg
//
LOCUS       DAOSUG010000413         2867 bp    DNA     linear   ENV 10-JUN-2024
DEFINITION  MAG TPA_asm: Micropruina sp. isolate AMR_MDS_2195
            NODE_14082_length_2867_cov_4.362731, whole genome shotgun sequence.
ACCESSION   DAOSUG010000413 DAOSUG010000000
VERSION     DAOSUG010000413.1
DBLINK      BioProject: PRJNA925477
            BioSample: SAMN32864262
            Sequence Read Archive: SRR4236673
KEYWORDS    WGS; ENV; Metagenome Assembled Genome; MAG; Third Party Data; TPA;
            TPA:assembly.
SOURCE      Micropruina sp. (wastewater metagenome)
  ORGANISM  Micropruina sp.
            Bacteria; Bacillati; Actinomycetota; Actinomycetes;
            Propionibacteriales; Nocardioidaceae; Micropruina.
REFERENCE   1  (bases 1 to 2867)
  AUTHORS   Abdulkadir,N., Saraiva,J.P., Zhang,J., Stolte,S., Gillor,O.,
            Harms,H. and Rocha,U.
  TITLE     Genome-centric analyses of 165 metagenomes show that mobile genetic
            elements are crucial for the transmission of antimicrobial
            resistance genes to pathogens in activated sludge and wastewater
  JOURNAL   Microbiol Spectr 12 (3), e0291823 (2024)
   PUBMED   38289113
REFERENCE   2  (bases 1 to 2867)
  AUTHORS   Abdulkadir,N.
  TITLE     Direct Submission
  JOURNAL   Submitted (24-MAR-2023) Environmental Microbiology, Helmholtz
            Center for Environmental Research -UFZ, Permoserstr 15, Leipzig
            04318, Germany
COMMENT     The annotation was added by the NCBI Prokaryotic Genome Annotation
            Pipeline (PGAP). Information about PGAP can be found here:
            https://www.ncbi.nlm.nih.gov/genome/annotation_prok/
            
            ##Genome-Assembly-Data-START##
            Assembly Method        :: SPAdes v. 3.11.1
            Genome Representation  :: Full
            Expected Final Version :: Yes
            Genome Coverage        :: Not Applicable
            Sequencing Technology  :: Illumina MiSeq
            ##Genome-Assembly-Data-END##
            
            ##Genome-Annotation-Data-START##
            Annotation Provider               :: NCBI
            Annotation Date                   :: 05/04/2023 04:57:10
            Annotation Pipeline               :: NCBI Prokaryotic Genome
                                                 Annotation Pipeline (PGAP)
            Annotation Method                 :: Best-placed reference protein
                                                 set; GeneMarkS-2+
            Annotation Software revision      :: 6.5
            Features Annotated                :: Gene; CDS; rRNA; tRNA; ncRNA
            Genes (total)                     :: 2,776
            CDSs (total)                      :: 2,750
            Genes (coding)                    :: 2,714
            CDSs (with protein)               :: 2,714
            Genes (RNA)                       :: 26
            tRNAs                             :: 24
            ncRNAs                            :: 2
            Pseudo Genes (total)              :: 36
            CDSs (without protein)            :: 36
            Pseudo Genes (ambiguous residues) :: 0 of 36
            Pseudo Genes (frameshifted)       :: 2 of 36
            Pseudo Genes (incomplete)         :: 32 of 36
            Pseudo Genes (internal stop)      :: 2 of 36
            ##Genome-Annotation-Data-END##
FEATURES             Location/Qualifiers
     source          1..2867
                     /organism="Micropruina sp."
                     /mol_type="genomic DNA"
                     /submitter_seqid="NODE_14082_length_2867_cov_4.362731"
                     /isolate="AMR_MDS_2195"
                     /isolation_source="Activated sludge of municipal
                     wastewater treatment plants"
                     /db_xref="taxon:2737536"
                     /environmental_sample
                     /geo_loc_name="Canada"
                     /lat_lon="49.25 N 123.1 W"
                     /collection_date="2015-06-16"
                     /metagenome_source="wastewater metagenome"
                     /note="metagenomic"
     gene            148..1188
                     /locus_tag="PLF56_11990"
     CDS             148..1188
                     /locus_tag="PLF56_11990"
                     /inference="COORDINATES: ab initio
                     prediction:GeneMarkS-2+"
                     /note="Derived by automated computational analysis using
                     gene prediction method: GeneMarkS-2+."
                     /codon_start=1
                     /transl_table=11
                     /product="hypothetical protein"
                     /protein_id="HOQ54318.1"
                     /translation="MPIGRRSLLLGAAGVALGGCVGPAQWRATALASEPQVALGLGEG
                     LLIGTAAPGLLGPDGAIPVTPTTPYGREVRWSALAVNEDRILGIGLAHGGAHGNARWS
                     VFTGSSRGITELEQPFDVFHGPESGQLSAAGFVGDRPVVIGSWRSDSAGNDVAVWLLD
                     GARWRRLRSSGTALAASATALPSAAAACGGDRLVLAGQVIELSPLRTRAAAWACTDPE
                     GDWMRIDLPGTGRTTTAQAITRLDDGWLVGGLAEGRAVAWRIAADLTAQLLDVPGLVA
                     AGTVRVAASHDGTALIVAGDGHRSEALVGRPGSWQRSVVPGDAPVAAAWGARAGVITR
                     AAGGATTLYELS"
     gene            1194..1589
                     /locus_tag="PLF56_11995"
     CDS             1194..1589
                     /locus_tag="PLF56_11995"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_020387810.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="pilus assembly protein CpaE"
                     /protein_id="HOQ54319.1"
                     /translation="MIDADLATRLRTAGLPWRPASGDRFTIPTGDFAGEVFVVAEMTI
                     EVQDVPGGNSVFKFNGTTEWALDSVDARRVLWLPREDQLRDQLGEAFVGLGAVSGGFA
                     VTLSDGSRHLDIDAERAYARAVLHLLAAS"
     gene            1592..2305
                     /locus_tag="PLF56_12000"
     CDS             1592..2305
                     /locus_tag="PLF56_12000"
                     /inference="COORDINATES: ab initio
                     prediction:GeneMarkS-2+"
                     /note="Derived by automated computational analysis using
                     gene prediction method: GeneMarkS-2+."
                     /codon_start=1
                     /transl_table=11
                     /product="hypothetical protein"
                     /protein_id="HOQ54320.1"
                     /translation="MLVERRFRGPNKSGNGGYSAGAVAAALGLPLGPAEVSLWSPPPL
                     DSPLTTRADGDGDGDGGGVTILHGEQIVATARPGPVEVAVPEPVSMAEATDAARRCPW
                     LVDHPYPQCFVCGTDRADGLRIFPGPVQGRRLAAAPWVPDESVANGGLVRPAVVWASI
                     DCPSLFGYGCFESWDALMLLGRFAAQVHRLPRVGEPCVVSGWALDRDGRKHDTAAALH
                     TAEGELLAFSRALWITLKN"
BASE COUNT          392 a         1002 c         1028 g          445 t
ORIGIN      
        1 gcgtgccgac atgcttgtcc agggtcgggc gacaagctcg accagcgaca aatcagcggc
       61 tcctgacacg gccgtggccc accccttctt cagcaccagc tcgccccgcg cgaccggaga
      121 tccttctccc gcgccgtacg atcaggcatg ccgatcgggc gccgttcgct actgctcggt
      181 gcggcgggcg tcgcactggg cggttgtgtg ggcccggcgc agtggcgcgc gaccgccctg
      241 gcgtccgagc ctcaggtagc actgggcctc ggcgaaggcc tgctgatcgg gaccgccgcc
      301 ccgggcctgc tgggtcctga cggtgcgatc cccgtcacac ccaccacacc gtatggacgc
      361 gaggtgcgtt ggtccgcgct ggccgtgaac gaggaccgca ttctcggcat cggcctggcg
      421 catggcggcg cacacgggaa cgcccgctgg agtgtgttca ccggctccag ccgcggcatc
      481 accgagttgg agcagccctt cgacgtgttc cacggccccg aatccggaca gctgtcggca
      541 gccggcttcg tgggcgatcg tccggtcgtg atcggctcgt ggcgaagtga ctcggcgggc
      601 aacgatgtcg ccgtgtggtt gttggacggg gcgcgctggc gcaggctccg gtcgtccggc
      661 acggcgttgg cggcgtccgc gaccgcactg ccctcggccg ctgcagcctg cgggggcgat
      721 cgtctggtgc tcgcgggtca ggtgatcgaa ctgagtccgt tgcgcacccg tgcggccgcc
      781 tgggcctgca ccgatcccga aggcgactgg atgcgcatcg acctgcccgg cactggccgg
      841 accaccaccg ctcaggccat cacccgcctt gacgacggct ggctggtggg cggcttggcg
      901 gagggccgag cggtcgcctg gcggatcgcc gccgatctca ctgcccagtt gctggacgta
      961 cccgggctgg ttgcggccgg caccgtccgg gtggccgcga gccacgacgg caccgcactg
     1021 atcgtggccg gcgacggcca tcgcagcgaa gcactagtcg gtcgccccgg cagttggcag
     1081 cgctcggtgg tacccggcga cgccccggtc gcggcggcgt ggggagcacg ggccggcgtg
     1141 atcacccggg ccgccggcgg ggccaccacc ctgtacgaac tatcctgacc gccatgatcg
     1201 atgccgacct cgccacccgc ctgcgcaccg ccggcctgcc ctggcgaccg gccagcggcg
     1261 accggttcac cattcccacc ggcgacttcg ccggcgaggt gttcgtggtg gccgagatga
     1321 ccatcgaggt gcaggacgta cctggcggca actcggtgtt caagttcaac ggaaccaccg
     1381 agtgggcgct cgattcggtg gacgcccgcc gcgtgctctg gctgccccgg gaggatcagt
     1441 tgcgtgacca gctcggcgag gcgttcgtcg gactgggcgc ggtgtccggc gggttcgcgg
     1501 tcaccctcag cgacggctcc cggcatctcg acatcgacgc cgaacgcgcc tacgcacggg
     1561 cggtcctgca cctgttggcc gcctcgtgag cgtgctggtc gaacgccggt tccgcggccc
     1621 gaacaagtcc ggcaacggcg gctacagcgc cggcgcggtc gccgccgcgc tgggcctgcc
     1681 gctcggcccg gccgaggtga gcctgtggtc gccaccaccg ttggacagcc cgttgaccac
     1741 ccgcgccgac ggcgatggcg atggcgacgg tggcggtgtc acgatcctgc acggtgagca
     1801 gatcgttgcc accgctcgtc cgggcccggt ggaggtcgcc gtgcccgagc cggtgagcat
     1861 ggctgaagcc acggatgccg cgcggcgctg cccatggctg gtcgatcatc cctacccgca
     1921 gtgcttcgtc tgcggcaccg accgggccga cggcctgcgc atctttcccg gcccggtgca
     1981 gggacggcgc ctggccgccg ccccctgggt accggacgaa tccgtcgcca acggcggcct
     2041 ggtgcggccg gccgtggtct gggcgagcat cgactgcccg tcgctgttcg gctacggctg
     2101 cttcgagtcg tgggacgcgc tcatgctgct cggccggttc gcggcgcagg tgcaccgact
     2161 gccacgggtg ggtgagccgt gcgtggtgag cggctgggca ctggaccgcg acggacgcaa
     2221 gcacgacacc gcggcggccc tgcacaccgc ggagggcgag ctgctggcgt tcagcagagc
     2281 cctctggatc acgctcaaga actgagggaa acgcggatcg tttggtcgag ctggtcgaaa
     2341 ccgaggcgag caggcgtttc cacacgccgc atactcgtca tcccgggctc gccccacgat
     2401 ctcggctcac acagagatcc cggcatgcgc cgggatgacg ggatcgggaa tcgctggccg
     2461 agctggtcga aaccgatgcg ggcaggagtt tcgaccggcg ccgtactcgt catcccgggc
     2521 tcgccccggg atctcagctc acacagagat cccggcatgc gccgggatga cgggatcggg
     2581 aatcgctgcc cgagcgtcgg gaccgatgcg agcaggagtt tcgaccggcg ccgtactcgt
     2641 catcccgcgc ttgcccctgg atctcagcgc aggcaaagat cccggcatgc gccgggatga
     2701 cggggtaggg aatcgccggc cgagctggcc gaaaccgagg cgagcagggg tttccacaag
     2761 ccgctcactc gtcatcccgg gcttgctctg ggatctcggc tcacacagag atccggcatg
     2821 cgactctcct atgtttcgtc aaggggtgtt tgggtgtagg cgcgggt
//
LOCUS       DAOSUG010000414         2865 bp    DNA     linear   ENV 10-JUN-2024
DEFINITION  MAG TPA_asm: Micropruina sp. isolate AMR_MDS_2195
            NODE_14092_length_2865_cov_3.386477, whole genome shotgun sequence.
ACCESSION   DAOSUG010000414 DAOSUG010000000
VERSION     DAOSUG010000414.1
DBLINK      BioProject: PRJNA925477
            BioSample: SAMN32864262
            Sequence Read Archive: SRR4236673
KEYWORDS    WGS; ENV; Metagenome Assembled Genome; MAG; Third Party Data; TPA;
            TPA:assembly.
SOURCE      Micropruina sp. (wastewater metagenome)
  ORGANISM  Micropruina sp.
            Bacteria; Bacillati; Actinomycetota; Actinomycetes;
            Propionibacteriales; Nocardioidaceae; Micropruina.
REFERENCE   1  (bases 1 to 2865)
  AUTHORS   Abdulkadir,N., Saraiva,J.P., Zhang,J., Stolte,S., Gillor,O.,
            Harms,H. and Rocha,U.
  TITLE     Genome-centric analyses of 165 metagenomes show that mobile genetic
            elements are crucial for the transmission of antimicrobial
            resistance genes to pathogens in activated sludge and wastewater
  JOURNAL   Microbiol Spectr 12 (3), e0291823 (2024)
   PUBMED   38289113
REFERENCE   2  (bases 1 to 2865)
  AUTHORS   Abdulkadir,N.
  TITLE     Direct Submission
  JOURNAL   Submitted (24-MAR-2023) Environmental Microbiology, Helmholtz
            Center for Environmental Research -UFZ, Permoserstr 15, Leipzig
            04318, Germany
COMMENT     The annotation was added by the NCBI Prokaryotic Genome Annotation
            Pipeline (PGAP). Information about PGAP can be found here:
            https://www.ncbi.nlm.nih.gov/genome/annotation_prok/
            
            ##Genome-Assembly-Data-START##
            Assembly Method        :: SPAdes v. 3.11.1
            Genome Representation  :: Full
            Expected Final Version :: Yes
            Genome Coverage        :: Not Applicable
            Sequencing Technology  :: Illumina MiSeq
            ##Genome-Assembly-Data-END##
            
            ##Genome-Annotation-Data-START##
            Annotation Provider               :: NCBI
            Annotation Date                   :: 05/04/2023 04:57:10
            Annotation Pipeline               :: NCBI Prokaryotic Genome
                                                 Annotation Pipeline (PGAP)
            Annotation Method                 :: Best-placed reference protein
                                                 set; GeneMarkS-2+
            Annotation Software revision      :: 6.5
            Features Annotated                :: Gene; CDS; rRNA; tRNA; ncRNA
            Genes (total)                     :: 2,776
            CDSs (total)                      :: 2,750
            Genes (coding)                    :: 2,714
            CDSs (with protein)               :: 2,714
            Genes (RNA)                       :: 26
            tRNAs                             :: 24
            ncRNAs                            :: 2
            Pseudo Genes (total)              :: 36
            CDSs (without protein)            :: 36
            Pseudo Genes (ambiguous residues) :: 0 of 36
            Pseudo Genes (frameshifted)       :: 2 of 36
            Pseudo Genes (incomplete)         :: 32 of 36
            Pseudo Genes (internal stop)      :: 2 of 36
            ##Genome-Annotation-Data-END##
FEATURES             Location/Qualifiers
     source          1..2865
                     /organism="Micropruina sp."
                     /mol_type="genomic DNA"
                     /submitter_seqid="NODE_14092_length_2865_cov_3.386477"
                     /isolate="AMR_MDS_2195"
                     /isolation_source="Activated sludge of municipal
                     wastewater treatment plants"
                     /db_xref="taxon:2737536"
                     /environmental_sample
                     /geo_loc_name="Canada"
                     /lat_lon="49.25 N 123.1 W"
                     /collection_date="2015-06-16"
                     /metagenome_source="wastewater metagenome"
                     /note="metagenomic"
     gene            complement(<1..438)
                     /locus_tag="PLF56_12005"
     CDS             complement(<1..438)
                     /locus_tag="PLF56_12005"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_017935282.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="SDR family NAD(P)-dependent oxidoreductase"
                     /protein_id="HOQ54321.1"
                     /translation="MTSTEAVSGRSAVVTGGNKGIGAAIVQALLADGHRVASFSRGGD
                     APEGALGIACDVTDGAQVEAAFKAAEEAHGPTEILVANSGVTKDTLILRMSEADWDLV
                     IDTNLKGAFRCAKRASRSMLRRRWGRIIFTGSVVGLYGSPGQVN"
     gene            495..809
                     /locus_tag="PLF56_12010"
     CDS             495..809
                     /locus_tag="PLF56_12010"
                     /inference="COORDINATES: protein motif:HMM:NF022742.2"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="DUF3099 domain-containing protein"
                     /protein_id="HOQ54322.1"
                     /translation="MSNAAQGRPVLITSARRAKSLDADERNRRYLLTMAVRVACFIGG
                     VLSPSPWNWVLFVGAAVIPPIAVVLANAVDRRQPVVAKDAEASDRPELGMGTVITGSV
                     ED"
     gene            813..1721
                     /locus_tag="PLF56_12015"
     CDS             813..1721
                     /locus_tag="PLF56_12015"
                     /inference="COORDINATES: protein motif:HMM:NF014193.2"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="SURF1 family protein"
                     /protein_id="HOQ54323.1"
                     /translation="MPGLGTMGAGAPSTRPWVRWIVMGLLVAALAVAFVNLGRWQLDR
                     LQQRRDNNGIVVAHESAPVVPWTSVFTHVLTDADQWQRVTVTGTFDPAHSYVVRYRSN
                     DDATGWEIITPLRTGSGDVLVSRGFAARPADQDFPRVAPAPPSGEVTIVGYVRRNEQG
                     GADAMTPADGAVRLINSDAVAATVGYPLVNGYIGAITITPPASDGLVPVHPPELTEGS
                     HFSYALQWFTFAGIAAFGLVVLIRSDLRDRRRAVAQAARAEEEMRAEATAQAETAAQA
                     ETAAQARQAAQAPTTTDADEATADAH"
     gene            1835..2593
                     /locus_tag="PLF56_12020"
     CDS             1835..2593
                     /locus_tag="PLF56_12020"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_013862361.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="SDR family oxidoreductase"
                     /protein_id="HOQ54324.1"
                     /translation="MDLGLTDRVFVVTAASGGLGRAGAEHLVAEGARVVLVARRVEVL
                     DELVHQLGADQAVALAGDLSDPCTAPSDADLALATFGRLDGALISVGGPPKGTVLDTS
                     DDQWHEAFDSVFLAGLRVTRAVVAAATGPVALALVLSSSARTPLPAMAPSNGLRPGLA
                     VLIKQLADEIGPAGGRVVGLLPGTVATERIDFLYGQTPDPGAARANAEASIPLRRLGE
                     PAEFARVATFLLSPAASYVTGSLVAIDGGAMRAL"
     gene            2604..>2865
                     /locus_tag="PLF56_12025"
     CDS             2604..>2865
                     /locus_tag="PLF56_12025"
                     /inference="COORDINATES: ab initio
                     prediction:GeneMarkS-2+"
                     /note="Derived by automated computational analysis using
                     gene prediction method: GeneMarkS-2+."
                     /codon_start=1
                     /transl_table=11
                     /product="hypothetical protein"
                     /protein_id="HOQ54325.1"
                     /translation="MSLHCAHGLLRCPHCAGDLSADDARWVCPQRHSFDVAKQGYLNL
                     ADGPEPANADTAAMLDVRARVQASGLFDFVSGALAAQVPLTGV"
BASE COUNT          405 a         1004 c         1018 g          438 t
ORIGIN      
        1 attcacctgg ccgggcgagc cgtacaggcc gaccaccgag ccggtgaaga tgatgcggcc
       61 ccagcgccgg cgcaacatgg agcgactggc ccgcttggcg cagcggaagg cacccttgag
      121 gttggtgtcg atcaccaggt cccaatccgc ctcgctcatg cgcaggatca gggtgtcctt
      181 cgtgacaccc gagttggcga ccagaatctc cgttggcccg tgcgcctctt cggcggcctt
      241 gaaggcggcc tcgacctgag cgccgtccgt cacgtcgcag gcgatgccca acgcaccctc
      301 gggtgcgtcg ccgccgcggg agaaactggc cacccggtgg ccgtccgcga gcagggcctg
      361 cacaatggcc gctccgatgc ctttgttacc tccggtgacc acggcgctgc ggccgctcac
      421 cgcctcggtt gaagtcacgg agcagaccct agcggaggag gctgcgcgtg ggcatgacga
      481 catacagtgt cacagtgagt aacgccgcgc agggccgtcc ggtcctgatc acgagcgccc
      541 ggcgggccaa gtcgctggac gccgacgaac gcaaccggcg ctacctgctc acgatggccg
      601 tccgggtggc ctgcttcatc ggcggcgtgc tgtcgccgag cccatggaac tgggtgttgt
      661 tcgtcggcgc ggccgtcatt ccccccatcg cggtggtgct cgccaacgcc gtcgatcgcc
      721 gccaacccgt cgtggcgaag gacgcggagg cgtccgatcg tccggagttg ggcatgggca
      781 ccgtgatcac cggttcggtg gaggactgac tcgtgcctgg cctgggaacg atgggcgcgg
      841 gcgccccgag cacccgcccc tgggtgcgct ggatcgtgat ggggttgctg gttgccgccc
      901 tggcggtggc cttcgtcaat ctcggccgct ggcagctcga ccggttgcag caacgccgcg
      961 acaacaacgg catcgtggtc gcgcacgaga gcgcgccggt ggtcccgtgg acctcggtgt
     1021 tcacccacgt actcaccgat gccgaccagt ggcaacgggt caccgtcacc ggcaccttcg
     1081 acccggccca ttcctacgtc gtccggtacc ggtcgaatga cgacgccacc ggctgggaga
     1141 tcatcacccc gctgcgcacc ggctccggtg acgtgctggt gagccggggg ttcgccgcac
     1201 gtccggccga tcaggacttt ccgcgggtgg cgccggcacc gccgtccggt gaggtgacga
     1261 tcgtggggta tgtgcgccgc aacgagcagg gcggtgccga cgcgatgacg cccgccgatg
     1321 gtgccgtgcg gctgatcaac tccgatgcgg tggcggcgac ggtcggctac cccctggtga
     1381 acgggtacat cggcgccatc accatcactc ccccggcctc ggacggcctg gtccctgtgc
     1441 atccgccgga gctgaccgag gggtcgcact tcagttacgc cctgcagtgg ttcaccttcg
     1501 ccggcatcgc cgccttcggc ctggtcgtgt tgatccgctc cgacctgcgc gaccggcgcc
     1561 gggccgtggc gcaggccgct cgggcggaag aggaaatgcg agcggaggcg acggcgcagg
     1621 ccgagacagc ggcgcaggcc gagacagcgg cgcaggcgag gcaagcggcg caggcaccga
     1681 cgacgaccga cgccgacgag gcaacggcgg atgctcacta gcctgaaaac cctgcactga
     1741 tcacacaccc ctgcctcggc acggcggaca ggctcgcact ggtgcaggga tttcaggatg
     1801 ccgctcgaca acgccgggtg gttagggtgg ccacatggac ctgggactga ccgatcgggt
     1861 gttcgtcgtc accgcggcga gtggcggcct gggccgcgcc ggcgccgaac acctggtcgc
     1921 cgagggtgcc agggtggtgc tcgtggcccg gcgcgtcgag gtgctggacg agctggtaca
     1981 ccagctgggc gccgatcaag ccgtcgcact ggccggcgac ctgagcgatc cgtgcacggc
     2041 accctcggac gccgacctcg ccctggccac cttcggacgg ctcgacgggg ccctgatcag
     2101 cgtgggcggc cctcccaagg gcacggtgct cgacacctct gacgaccagt ggcacgaggc
     2161 gttcgactca gtgttcctgg ccgggttgcg ggtgactcga gccgtggtgg cggcggcgac
     2221 cggcccggtg gcgctcgccc tggtgttgtc gtcatcggcg agaacccccc tgcccgccat
     2281 ggcaccctcg aacgggctgc gacccggcct ggcggtgttg atcaagcaac tcgcggacga
     2341 gatcggcccg gccggtggcc gggtggtcgg actgctgccc ggcaccgtcg caaccgagcg
     2401 gatcgacttc ctgtacggcc agacccccga tccgggcgcc gcgcgggcga acgccgaggc
     2461 gtccatacct ctgcgtcgcc tcggtgagcc ggccgagttc gcccgggtgg cgacctttct
     2521 gctctcgccc gccgcgtcgt acgtgaccgg atccctggtc gcgatcgacg gtggcgcgat
     2581 gcgggcactg tgagtgcgcg ccggtgagcc tgcactgcgc gcacgggctg ctgcgctgcc
     2641 cgcactgcgc tggtgatctg agcgccgacg acgctcgctg ggtgtgccct caacggcaca
     2701 gcttcgatgt ggccaaacag gggtacctga acctggcgga cggccccgag cccgccaatg
     2761 ccgacaccgc cgccatgctg gacgtccggg ccagggtgca ggcgtccgga ctgttcgact
     2821 tcgtctccgg cgccctggcc gcgcaggtgc ccctgaccgg tgtcg
//
LOCUS       DAOSUG010000415         2858 bp    DNA     linear   ENV 10-JUN-2024
DEFINITION  MAG TPA_asm: Micropruina sp. isolate AMR_MDS_2195
            NODE_14134_length_2858_cov_4.157688, whole genome shotgun sequence.
ACCESSION   DAOSUG010000415 DAOSUG010000000
VERSION     DAOSUG010000415.1
DBLINK      BioProject: PRJNA925477
            BioSample: SAMN32864262
            Sequence Read Archive: SRR4236673
KEYWORDS    WGS; ENV; Metagenome Assembled Genome; MAG; Third Party Data; TPA;
            TPA:assembly.
SOURCE      Micropruina sp. (wastewater metagenome)
  ORGANISM  Micropruina sp.
            Bacteria; Bacillati; Actinomycetota; Actinomycetes;
            Propionibacteriales; Nocardioidaceae; Micropruina.
REFERENCE   1  (bases 1 to 2858)
  AUTHORS   Abdulkadir,N., Saraiva,J.P., Zhang,J., Stolte,S., Gillor,O.,
            Harms,H. and Rocha,U.
  TITLE     Genome-centric analyses of 165 metagenomes show that mobile genetic
            elements are crucial for the transmission of antimicrobial
            resistance genes to pathogens in activated sludge and wastewater
  JOURNAL   Microbiol Spectr 12 (3), e0291823 (2024)
   PUBMED   38289113
REFERENCE   2  (bases 1 to 2858)
  AUTHORS   Abdulkadir,N.
  TITLE     Direct Submission
  JOURNAL   Submitted (24-MAR-2023) Environmental Microbiology, Helmholtz
            Center for Environmental Research -UFZ, Permoserstr 15, Leipzig
            04318, Germany
COMMENT     The annotation was added by the NCBI Prokaryotic Genome Annotation
            Pipeline (PGAP). Information about PGAP can be found here:
            https://www.ncbi.nlm.nih.gov/genome/annotation_prok/
            
            ##Genome-Assembly-Data-START##
            Assembly Method        :: SPAdes v. 3.11.1
            Genome Representation  :: Full
            Expected Final Version :: Yes
            Genome Coverage        :: Not Applicable
            Sequencing Technology  :: Illumina MiSeq
            ##Genome-Assembly-Data-END##
            
            ##Genome-Annotation-Data-START##
            Annotation Provider               :: NCBI
            Annotation Date                   :: 05/04/2023 04:57:10
            Annotation Pipeline               :: NCBI Prokaryotic Genome
                                                 Annotation Pipeline (PGAP)
            Annotation Method                 :: Best-placed reference protein
                                                 set; GeneMarkS-2+
            Annotation Software revision      :: 6.5
            Features Annotated                :: Gene; CDS; rRNA; tRNA; ncRNA
            Genes (total)                     :: 2,776
            CDSs (total)                      :: 2,750
            Genes (coding)                    :: 2,714
            CDSs (with protein)               :: 2,714
            Genes (RNA)                       :: 26
            tRNAs                             :: 24
            ncRNAs                            :: 2
            Pseudo Genes (total)              :: 36
            CDSs (without protein)            :: 36
            Pseudo Genes (ambiguous residues) :: 0 of 36
            Pseudo Genes (frameshifted)       :: 2 of 36
            Pseudo Genes (incomplete)         :: 32 of 36
            Pseudo Genes (internal stop)      :: 2 of 36
            ##Genome-Annotation-Data-END##
FEATURES             Location/Qualifiers
     source          1..2858
                     /organism="Micropruina sp."
                     /mol_type="genomic DNA"
                     /submitter_seqid="NODE_14134_length_2858_cov_4.157688"
                     /isolate="AMR_MDS_2195"
                     /isolation_source="Activated sludge of municipal
                     wastewater treatment plants"
                     /db_xref="taxon:2737536"
                     /environmental_sample
                     /geo_loc_name="Canada"
                     /lat_lon="49.25 N 123.1 W"
                     /collection_date="2015-06-16"
                     /metagenome_source="wastewater metagenome"
                     /note="metagenomic"
     gene            <1..769
                     /locus_tag="PLF56_12030"
     CDS             <1..769
                     /locus_tag="PLF56_12030"
                     /inference="COORDINATES: ab initio
                     prediction:GeneMarkS-2+"
                     /note="Derived by automated computational analysis using
                     gene prediction method: GeneMarkS-2+."
                     /codon_start=2
                     /transl_table=11
                     /product="hypothetical protein"
                     /protein_id="HOQ54326.1"
                     /translation="AMVFALAGTFSARAAGDGTPGRINWRGEIAEGFTWLWHNRLIRT
                     LAILLGLLNLLSNLAFVTAVLFVQDVLGLFEGWQFGLITTGFAAGAVIGSLTAEGITA
                     RLTPGVALLVSITGIGASLLIIGLVPFAVVFWVCGVAEGIFVVIWNVITVSLRQRLVP
                     DRLLGRVNSVYRFFGWGTIAIGTVLGGALVSLGETMMGRDWALRGVFLLAGLGHLAVL
                     GYAWRRTSTKQIRTAEDAAERAAAAEAAAGPAATTDA"
     gene            complement(770..1945)
                     /locus_tag="PLF56_12035"
     CDS             complement(770..1945)
                     /locus_tag="PLF56_12035"
                     /EC_number="2.3.1.29"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_002521182.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_function: GO:0008890 - glycine C-acetyltransferase
                     activity [Evidence IEA];
                     GO_function: GO:0030170 - pyridoxal phosphate binding
                     [Evidence IEA];
                     GO_process: GO:0009058 - biosynthetic process [Evidence
                     IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="glycine C-acetyltransferase"
                     /protein_id="HOQ54327.1"
                     /translation="MRDDLTHTLDEIRAAGLYKNERELATPQAAHIATPDGAALNFCA
                     NNYLGLADHPDVVAAAHAGLDDWGFGMASVRFICGTQSQHRELERELAAFLGTDDAIL
                     YSSCFDANGGVFETLFGADDAIISDELNHASIIDGVRLSKAARYRYRNADLADLQAQL
                     VAAGGARHRVIVTDGVFSMDGSYAPLPGICDLAERHDALVFVDDSHAVGFVGEGGGGT
                     PELFGVRDRVDILTGTLGKALGGASGGYVAAHAEIVELLRQRSRPYLFSNSVAPSVVA
                     GSRKALELVAGSAQARQRLNANAVLFRELMSAEGFDLLPGNHPIVPVMFPGDDGARRA
                     TQIADHMLSEGVYVIAFSFPVVPRGRARIRVQLSAGHSTSDVETCVRAFLRARAAVG"
     gene            complement(1968..>2858)
                     /locus_tag="PLF56_12040"
     CDS             complement(1968..>2858)
                     /locus_tag="PLF56_12040"
                     /inference="COORDINATES: ab initio
                     prediction:GeneMarkS-2+"
                     /note="Derived by automated computational analysis using
                     gene prediction method: GeneMarkS-2+."
                     /codon_start=1
                     /transl_table=11
                     /product="hypothetical protein"
                     /protein_id="HOQ54328.1"
                     /translation="MLLAAVGVSLLVMLGMSQAYRPQSPAEIAQAERDYADAQQQAEQ
                     DRKDCIDSGQSAQDCDTWTPQRSDFVYEPPTYASLVGNGAQTATMIAQLAALVVAASF
                     MGAEFKSGSLATWLTYVPRRTPVFASKAVVAVIGSAVLGAVVHAIMQIGMVAYAWNWL
                     GASGLQGAAAEWGIVGRGLIVTAGAGLLGYCLATLFGSTIAPLGALLGIVVAQWMAGI
                     VTLLVGEMPWLIQLMPDNNLNAFVNGQFETLFVVRGTAGEEVEQVIRIGLGQASLYLG
                     CLLIVAVASALWSFTRREVR"
BASE COUNT          441 a          991 c          982 g          444 t
ORIGIN      
        1 cgcgatggtg ttcgccctgg cgggcacctt cagcgcccgc gcggccggcg acggcacgcc
       61 cggccggatc aattggcgcg gcgagatcgc cgagggtttc acctggctgt ggcacaaccg
      121 gctgattcgc accctcgcga tcctgctggg tctgttgaac ctgctgagca acctggcgtt
      181 cgtcaccgcg gtgctgttcg tgcaggacgt gctggggctg ttcgagggct ggcagttcgg
      241 cctgatcacc accggtttcg ccgccggtgc ggtgatcggc tcgctgaccg ccgaagggat
      301 caccgcccgg ctgacgcccg gcgtcgcgct gctggtctcg atcaccggca tcggagcctc
      361 gctgctgatc atcggcctgg tgccgttcgc ggtggtgttc tgggtgtgcg gggtggccga
      421 gggcatcttc gtggtgatct ggaacgtgat caccgtgtcg ttgcgccagc ggttggtgcc
      481 cgaccggctg ctcggccggg tcaactcggt gtaccgcttc ttcggctggg gcaccatcgc
      541 gatcggcact gtgctgggcg gtgcgctggt cagcctcggc gaaaccatga tgggtcgcga
      601 ctgggcgctg cgcggggtct ttctgctggc cggtctgggg cacctggccg tgctgggcta
      661 cgcgtggagg cgcaccagca ccaagcagat ccggacggcc gaggatgcag ccgaacgggc
      721 cgccgcggca gaagcggccg cgggcccggc cgccacaacc gacgcctgat caacccaccg
      781 cggcccgggc acggaggaac gcccggacgc aggtctcgac gtcggacgtg ctgtgcccgg
      841 ccgacagctg gacccggatg cgggcccgtc cgcgcggaac gaccggaaag ctgaacgcga
      901 tcacgtagac gccctcgctc agcatgtgat cggcgatctg ggtggcccgg cgggcgccgt
      961 cgtcgccggg aaacatgacc ggcacgatcg ggtggttacc aggcagcaga tcgaacccct
     1021 cggcgctcat caactcgcgg aacagcaccg cgttcgcgtt cagccgctgc cgggcctgcg
     1081 ccgacccggc gaccagttcg agcgccttgc gcgaaccggc caccacgctg ggggccaccg
     1141 agttgctgaa cagataaggc cgggagcgct gccgcagcag ctccacgatc tcggcgtgcg
     1201 cggccacata tccaccggac gccccaccca acgccttgcc cagcgtgccg gtcagaatgt
     1261 cgacgcggtc acggacgccg aacagttccg gggtgccgcc gccaccctcg ccgacgaacc
     1321 cgacggcgtg cgagtcgtcc acgaacacca gggcgtcgtg gcgttccgcc aggtcgcaga
     1381 tgccgggcaa cggggcgtag gagccgtcca tcgaaaagac gccgtcggtg acgatcaccc
     1441 ggtgccgggc gccgccggcc gccaccagtt gcgcctgcag atcggccaga tcggcgttgc
     1501 ggtagcggta acgcgcggcc ttgctcagcc gtaccccgtc gatgatcgag gcgtggttga
     1561 gctcgtcgct gatgatcgca tcgtcggcgc cgaacagggt ctcgaacact cccccgttgg
     1621 cgtcgaagca cgacgagtac agaatcgcgt cgtcggtgcc caggaacgcg gcaagttcgc
     1681 gctccagctc gcggtgctgg ctctgggtgc cgcagatgaa gcgcacggag gccatgccga
     1741 agccccagtc gtcgaggccc gcgtgggcgg cggcgaccac gtccgggtgg tcggccagtc
     1801 ccaggtagtt gttggcgcag aagttgagcg ccgcaccgtc cggggtggcg atgtgggcgg
     1861 cttggggcgt ggccagctcg cgttcgttct tgtacaggcc ggctgcccgg atctcgtcca
     1921 gggtgtgggt caggtcgtcg cgcagagatc catacacgtc cgcgacgcta gcgcacctcg
     1981 cgccgcgtga agctccacag cgcggacgcc accgcaacga tcagcaggca gcccaggtac
     2041 agcgacgcct ggcccaatcc gattcggatg acctgctcca cttcttcgcc ggcggtgccc
     2101 cgcaccacga acaaggtctc gaactggccg ttgacgaagg cgttgaggtt gttgtcgggc
     2161 atcagctgga tcagccacgg catttcaccc acgagcagcg tgacgatgcc ggccatccat
     2221 tgcgcgacca caatgcccag cagcgccccc agcggagcga tggtgctgcc gaacagcgtg
     2281 gccaggcagt agcccagcag gccggcacct gcggtgacga tcaggccacg cccgactatg
     2341 ccccactccg ctgcggcccc ctgcagtccg gacgccccga gccagttcca cgcgtacgcc
     2401 accatgccga tctgcatgat ggcgtggacg accgcaccca acaccgccga gccgatcacc
     2461 gcgaccaccg ccttgctggc gaacactggt gttcggcgcg gcacgtaggt cagccaggtg
     2521 gccaggctgc ccgacttgaa ctccgccccc atgaacgacg ccgccaccac cagggcggcg
     2581 agctgggcga tcatcgttgc ggtctgcgca ccgttgccga ccagactcgc gtaggtgggc
     2641 ggctcgtaga cgaaatcgct gcgctgcggc gtccaggtgt cacagtcctg ggccgattgg
     2701 cccgagtcga tgcagtcctt gcgatcctgt tcggcttgct gctgcgcatc ggcgtagtcg
     2761 cgctcggcct gagcaatctc ggcagggctc tgcggacggt acgcctggct catgcccagc
     2821 atcaccagca gcgacacccc cacggcagcg agcagcat
//
LOCUS       DAOSUG010000416         2856 bp    DNA     linear   ENV 10-JUN-2024
DEFINITION  MAG TPA_asm: Micropruina sp. isolate AMR_MDS_2195
            NODE_14148_length_2856_cov_5.089254, whole genome shotgun sequence.
ACCESSION   DAOSUG010000416 DAOSUG010000000
VERSION     DAOSUG010000416.1
DBLINK      BioProject: PRJNA925477
            BioSample: SAMN32864262
            Sequence Read Archive: SRR4236673
KEYWORDS    WGS; ENV; Metagenome Assembled Genome; MAG; Third Party Data; TPA;
            TPA:assembly.
SOURCE      Micropruina sp. (wastewater metagenome)
  ORGANISM  Micropruina sp.
            Bacteria; Bacillati; Actinomycetota; Actinomycetes;
            Propionibacteriales; Nocardioidaceae; Micropruina.
REFERENCE   1  (bases 1 to 2856)
  AUTHORS   Abdulkadir,N., Saraiva,J.P., Zhang,J., Stolte,S., Gillor,O.,
            Harms,H. and Rocha,U.
  TITLE     Genome-centric analyses of 165 metagenomes show that mobile genetic
            elements are crucial for the transmission of antimicrobial
            resistance genes to pathogens in activated sludge and wastewater
  JOURNAL   Microbiol Spectr 12 (3), e0291823 (2024)
   PUBMED   38289113
REFERENCE   2  (bases 1 to 2856)
  AUTHORS   Abdulkadir,N.
  TITLE     Direct Submission
  JOURNAL   Submitted (24-MAR-2023) Environmental Microbiology, Helmholtz
            Center for Environmental Research -UFZ, Permoserstr 15, Leipzig
            04318, Germany
COMMENT     The annotation was added by the NCBI Prokaryotic Genome Annotation
            Pipeline (PGAP). Information about PGAP can be found here:
            https://www.ncbi.nlm.nih.gov/genome/annotation_prok/
            
            ##Genome-Assembly-Data-START##
            Assembly Method        :: SPAdes v. 3.11.1
            Genome Representation  :: Full
            Expected Final Version :: Yes
            Genome Coverage        :: Not Applicable
            Sequencing Technology  :: Illumina MiSeq
            ##Genome-Assembly-Data-END##
            
            ##Genome-Annotation-Data-START##
            Annotation Provider               :: NCBI
            Annotation Date                   :: 05/04/2023 04:57:10
            Annotation Pipeline               :: NCBI Prokaryotic Genome
                                                 Annotation Pipeline (PGAP)
            Annotation Method                 :: Best-placed reference protein
                                                 set; GeneMarkS-2+
            Annotation Software revision      :: 6.5
            Features Annotated                :: Gene; CDS; rRNA; tRNA; ncRNA
            Genes (total)                     :: 2,776
            CDSs (total)                      :: 2,750
            Genes (coding)                    :: 2,714
            CDSs (with protein)               :: 2,714
            Genes (RNA)                       :: 26
            tRNAs                             :: 24
            ncRNAs                            :: 2
            Pseudo Genes (total)              :: 36
            CDSs (without protein)            :: 36
            Pseudo Genes (ambiguous residues) :: 0 of 36
            Pseudo Genes (frameshifted)       :: 2 of 36
            Pseudo Genes (incomplete)         :: 32 of 36
            Pseudo Genes (internal stop)      :: 2 of 36
            ##Genome-Annotation-Data-END##
FEATURES             Location/Qualifiers
     source          1..2856
                     /organism="Micropruina sp."
                     /mol_type="genomic DNA"
                     /submitter_seqid="NODE_14148_length_2856_cov_5.089254"
                     /isolate="AMR_MDS_2195"
                     /isolation_source="Activated sludge of municipal
                     wastewater treatment plants"
                     /db_xref="taxon:2737536"
                     /environmental_sample
                     /geo_loc_name="Canada"
                     /lat_lon="49.25 N 123.1 W"
                     /collection_date="2015-06-16"
                     /metagenome_source="wastewater metagenome"
                     /note="metagenomic"
     gene            <1..1376
                     /locus_tag="PLF56_12045"
     CDS             <1..1376
                     /locus_tag="PLF56_12045"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_014847819.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=3
                     /transl_table=11
                     /product="ATP-binding protein"
                     /protein_id="HOQ54329.1"
                     /translation="LLALPEDQWFDRKSIRVRAQDLDRPLTAFANAEGGVIVIGLSDG
                     RVEGIRAQASKQNAIRQAALDFTVPPVRAHVEQVGCINDAGEVDALLVIRVDPGEMVH
                     ETRAGDVYLRVGDESRKLGFAARQELEFDKGQGQYDGRPVPGVRIADLDRKLLHNYRS
                     RVGASSDLKLFRARSLVTVKDELTNAGYLLFAPRPQDLFPEAYIRVIRFLTSERGTGA
                     RLGVEEGSDIRIEGPIPRAIQEADRVISELIPKRRALAESGLFEARPIVPREAWLEGV
                     VNAAIHRSYSLSGDHIRVEIYPDRVEIESPGRFPGLANPALPLEIPRFARNPRIARVC
                     ADLRIGQELGEGIKRMFDEMRIVGLRDPAYRQTAGSVRLTLAALPRLPATQAARLPKG
                     SQQILDALRTAGVAGLGSGEVAEAAGLSRPAANARLKALREEGLIVWHGKSSKDPRAR
                     WTLAEGS"
     gene            complement(1309..1836)
                     /locus_tag="PLF56_12050"
     CDS             complement(1309..1836)
                     /locus_tag="PLF56_12050"
                     /inference="COORDINATES: protein motif:HMM:NF020332.2"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="relaxase domain-containing protein"
                     /protein_id="HOQ54330.1"
                     /translation="MHDQAVTDALTYLESRALFTRQGTDGVRRLPVRGMIAARFVHRD
                     SRAGDPDLHTHVAISNKVQTLAGDWLAIDATNPVRGQGDHLRGHYQPDRRPARPRPGH
                     GTNASAIPGRTQTSCSAARRRGEPSSRSWSRSNRRRRADRHAALPEEPHPRISSLRQV
                     SSELADPWRTCRAKR"
     gene            complement(1806..2429)
                     /locus_tag="PLF56_12055"
     CDS             complement(1806..2429)
                     /locus_tag="PLF56_12055"
                     /inference="COORDINATES: protein motif:HMM:NF020332.2"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="relaxase domain-containing protein"
                     /protein_id="HOQ54331.1"
                     /translation="MAIGLQKLSAGSGYEYLTRQVAALDVTGRGHISLAEYYAAKGEA
                     PGRWWGAGLDAVDLALGGEVTAEQMKLLFGAGLNPTTGERLGRAYSVFAGEPSPFETE
                     LASRLTAWQDTHGLEPAAPIPQQVRSQLRTELGRERFLAQHGVPPEGPRELHAFITKA
                     ISHPRVPVAGFDLTLSPPKSVSALWRLPTRSWRPGCGRCTTKPSPTR"
BASE COUNT          475 a          977 c          940 g          464 t
ORIGIN      
        1 gcctcttggc gttgccggag gaccagtggt tcgatcgcaa gtcgattcgc gtccgggcgc
       61 aggacctcga caggccgctc accgccttcg ccaacgcaga gggcggggtc atcgtgatcg
      121 gactgtccga cgggcgagtg gaggggatcc gagcccaagc gtcgaagcag aacgccatcc
      181 ggcaagccgc actggacttc accgtccctc ctgtgcgcgc acacgtggag caggtcggct
      241 gcatcaacga cgccggcgaa gtggacgcgc tcctcgtgat tcgtgtcgat ccgggtgaga
      301 tggtccacga gactcgcgcc ggcgacgtgt acctccgcgt cggggacgag tcgcgcaagc
      361 tcgggttcgc cgctcgtcag gaactggagt tcgacaaggg gcagggccaa tacgatggcc
      421 ggccggtccc cggtgtgcgg atcgccgatc tggacaggaa gctcctccac aactaccggt
      481 cgcgtgtcgg agcttccagc gatctcaagc tgttccgcgc ccggagtctg gtcaccgtga
      541 aggacgaact caccaacgct ggctacttgc tgttcgcacc gcgtccgcaa gacctgttcc
      601 ctgaggcgta cattcgcgtg attcgctttc tcacctccga gcgcggcacg ggcgcccggc
      661 tgggggtcga ggaaggctct gacatccgca tcgaggggcc tatcccgcgt gcgatccagg
      721 aggcggatcg ggtgatcagt gagctgatac ccaagcggcg agcactggcc gagagcgggc
      781 tgttcgaggc ccgtccgatc gttccgcgtg aggcctggct ggaaggagtg gtcaacgccg
      841 cgatccatcg ttcttacagc ctgtccggcg accatatccg agtcgagatc taccccgacc
      901 gggtcgagat cgaaagcccg ggacgcttcc cgggcctggc caacccggca ctcccgctgg
      961 agatcccgcg gttcgcccgc aacccacgaa tcgctcgggt ttgtgcggac cttcgaatcg
     1021 gccaggaact gggggagggc atcaagcgaa tgttcgacga gatgcgaatt gtcggcctgc
     1081 gggatcccgc ctaccgccaa acagccggca gcgtccgact gactctcgcc gccctgcccc
     1141 gtctgcctgc tacccaggcc gcgcgcctcc ccaagggatc gcagcagatc ctggacgcac
     1201 tccgaaccgc tggagtcgcc ggactggggt caggggaagt ggccgaggcg gcagggctga
     1261 gccgccctgc cgcaaatgcc cgcctgaagg ccttgcgtga ggagggcctc atcgtttggc
     1321 acggcaagtc ctccaaggat ccgcgagctc gctggacact tgccgaaggg agctgatgcg
     1381 cggatgtggt tcttcgggca aggccgcgtg cctatcagca cgtcgtcgac ggttgctacg
     1441 cgaccatgac ctcgatgatg gctccccccg ccggcgtgca gccgaacatg acgtttgcgt
     1501 tcgcccgggt atcgcacttg cgttcgtccc atggccaggc cgaggtcgcg caggcctgcg
     1561 gtcaggctgg tagtggcctc gcagatggtc accttggccg cggacgggat tggttgcgtc
     1621 gatggcgagc cagtcaccgg cgagggtttg gaccttgttc gagatcgcta cgtgggtgtg
     1681 caggtccgga tcaccggccc gggaatcacg atgcacgaac cgggccgcga tcatgccccg
     1741 caccggcaac cggcggacac catccgtgcc ctgccgggtg aacaacgccc gagactccaa
     1801 atacgtcagc gcgtcggtga cggcttggtc gtgcaccgcc cgcaaccggg acgccagctc
     1861 cgggtcggca accgccacag tgccgagacg gacttgggtg gggacagggt gaggtcgaat
     1921 ccggcgaccg gcacccgggg atgggagatc gctttcgtga tgaacgcgtg cagttccctc
     1981 ggcccctccg gtggcacgcc gtgctgggcg aggaaccgct ctcggcccaa ctccgtgcgt
     2041 aactgggaac gaacctgctg ggggatcggc gcagcaggtt cgaggccgtg ggtgtcctgc
     2101 caagcggtca gccggctggc gagttcggtc tcgaacgggc tcggctcacc ggcgaacact
     2161 gagtaggccc ggccaagtct ctcgccggta gtcgggttca ggccggcacc gaacaggagc
     2221 ttcatctgct cggccgtcac ctctccgccc agggcgaggt cgaccgcgtc caagccggcg
     2281 ccccaccacc tgccgggagc ctcacccttg gcggcgtagt actcggccag gctgatgtgg
     2341 cccctgccgg tcacgtccag cgcggcgacc tgccgggtca ggtactcata gcccgaccca
     2401 gctgagagct tctgcagacc aatcgccacc ggacgcctcc cttcaccatt ccctaagccc
     2461 cgtcaggccc cgatcgggac gccggccggg ccacttcttc tctggttctc agcccgagac
     2521 ccgttcactt ccttaagccc cgaaaccggc ccgtttcgga cagcccagcc tggagaagtt
     2581 cgactccggc caggcgagcg ccggggccac tggtgacgcg tccgtcaatc agagcggacc
     2641 ggcgtttcca caaacgctcc ccctggcgtc cccagcgccg gtttcgagcc tcggcggcga
     2701 ggtgatcagt gcttgtggga agcacgttcc ccaacgttcc cccagtcgct gcatacaacc
     2761 gtctgcaggc acacgagaag ggcaccacag caccccccac ccaccccccc accccgggca
     2821 aggcagtcgt ggtgatcagc gccgatcagg tgaacg
//
LOCUS       DAOSUG010000417         2849 bp    DNA     linear   ENV 10-JUN-2024
DEFINITION  MAG TPA_asm: Micropruina sp. isolate AMR_MDS_2195
            NODE_14206_length_2849_cov_4.149964, whole genome shotgun sequence.
ACCESSION   DAOSUG010000417 DAOSUG010000000
VERSION     DAOSUG010000417.1
DBLINK      BioProject: PRJNA925477
            BioSample: SAMN32864262
            Sequence Read Archive: SRR4236673
KEYWORDS    WGS; ENV; Metagenome Assembled Genome; MAG; Third Party Data; TPA;
            TPA:assembly.
SOURCE      Micropruina sp. (wastewater metagenome)
  ORGANISM  Micropruina sp.
            Bacteria; Bacillati; Actinomycetota; Actinomycetes;
            Propionibacteriales; Nocardioidaceae; Micropruina.
REFERENCE   1  (bases 1 to 2849)
  AUTHORS   Abdulkadir,N., Saraiva,J.P., Zhang,J., Stolte,S., Gillor,O.,
            Harms,H. and Rocha,U.
  TITLE     Genome-centric analyses of 165 metagenomes show that mobile genetic
            elements are crucial for the transmission of antimicrobial
            resistance genes to pathogens in activated sludge and wastewater
  JOURNAL   Microbiol Spectr 12 (3), e0291823 (2024)
   PUBMED   38289113
REFERENCE   2  (bases 1 to 2849)
  AUTHORS   Abdulkadir,N.
  TITLE     Direct Submission
  JOURNAL   Submitted (24-MAR-2023) Environmental Microbiology, Helmholtz
            Center for Environmental Research -UFZ, Permoserstr 15, Leipzig
            04318, Germany
COMMENT     The annotation was added by the NCBI Prokaryotic Genome Annotation
            Pipeline (PGAP). Information about PGAP can be found here:
            https://www.ncbi.nlm.nih.gov/genome/annotation_prok/
            
            ##Genome-Assembly-Data-START##
            Assembly Method        :: SPAdes v. 3.11.1
            Genome Representation  :: Full
            Expected Final Version :: Yes
            Genome Coverage        :: Not Applicable
            Sequencing Technology  :: Illumina MiSeq
            ##Genome-Assembly-Data-END##
            
            ##Genome-Annotation-Data-START##
            Annotation Provider               :: NCBI
            Annotation Date                   :: 05/04/2023 04:57:10
            Annotation Pipeline               :: NCBI Prokaryotic Genome
                                                 Annotation Pipeline (PGAP)
            Annotation Method                 :: Best-placed reference protein
                                                 set; GeneMarkS-2+
            Annotation Software revision      :: 6.5
            Features Annotated                :: Gene; CDS; rRNA; tRNA; ncRNA
            Genes (total)                     :: 2,776
            CDSs (total)                      :: 2,750
            Genes (coding)                    :: 2,714
            CDSs (with protein)               :: 2,714
            Genes (RNA)                       :: 26
            tRNAs                             :: 24
            ncRNAs                            :: 2
            Pseudo Genes (total)              :: 36
            CDSs (without protein)            :: 36
            Pseudo Genes (ambiguous residues) :: 0 of 36
            Pseudo Genes (frameshifted)       :: 2 of 36
            Pseudo Genes (incomplete)         :: 32 of 36
            Pseudo Genes (internal stop)      :: 2 of 36
            ##Genome-Annotation-Data-END##
FEATURES             Location/Qualifiers
     source          1..2849
                     /organism="Micropruina sp."
                     /mol_type="genomic DNA"
                     /submitter_seqid="NODE_14206_length_2849_cov_4.149964"
                     /isolate="AMR_MDS_2195"
                     /isolation_source="Activated sludge of municipal
                     wastewater treatment plants"
                     /db_xref="taxon:2737536"
                     /environmental_sample
                     /geo_loc_name="Canada"
                     /lat_lon="49.25 N 123.1 W"
                     /collection_date="2015-06-16"
                     /metagenome_source="wastewater metagenome"
                     /note="metagenomic"
     gene            complement(<1..486)
                     /locus_tag="PLF56_12060"
     CDS             complement(<1..486)
                     /locus_tag="PLF56_12060"
                     /inference="COORDINATES: ab initio
                     prediction:GeneMarkS-2+"
                     /note="Derived by automated computational analysis using
                     gene prediction method: GeneMarkS-2+."
                     /codon_start=1
                     /transl_table=11
                     /product="hypothetical protein"
                     /protein_id="HOQ54332.1"
                     /translation="MVELPGGVVVLLLGLVLSYRNDPRTFRTGLFLLAILTWAVLAVV
                     GLLLDSAERRFDSTAAGVVLLVLLVLLLATVLILGVFLITTGVTLVRREGFAIRRLLS
                     VGLGVSMIGYVGSWLVVLALNEVAPALFLVAVGVPLAYLGFGFVAFLLYGTLYPAVMA
                     RR"
     gene            complement(547..1767)
                     /locus_tag="PLF56_12065"
     CDS             complement(547..1767)
                     /locus_tag="PLF56_12065"
                     /inference="COORDINATES: protein motif:HMM:NF019310.2"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_function: GO:0022857 - transmembrane transporter
                     activity [Evidence IEA];
                     GO_process: GO:0055085 - transmembrane transport [Evidence
                     IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="MFS transporter"
                     /protein_id="HOQ54333.1"
                     /translation="MTVSTVSRTVAGARTGTLLVFAANGFAFASWMSRVPDVKQLLAL
                     TPSMLAVLLLSVSVGALVGLPAAGRIAHRFGAVVTVRLGIATMAPGIVLAAAAVQAGL
                     PMYWTMPGLFLAGLGSGVWDVAQNLEGSIVEQALDRAIMPWFHAAFSGGTVLAALIGA
                     ALTWLGVPLLAHLSGAAVLTALIGWWGTTQFLPAFDEAGGPVAAAPSAPQRSAWTEPR
                     TLLIGLMVTAAAFAEGTANDWMAVAFVDGHQLDNALGVVALAVFLSFMTAGRILGTHL
                     LDRYGRVAVLRILFGAAIIGCLMVVFGNVWVAFVGCAIWGLGASLGFPVGMSAAADDP
                     ARAPMRLSVVSTIGYTAFLAGPPLLGFLGDHFGVLRALLVVGAVSVLAMLVVPVARPL
                     EPDPAQPASNDASA"
     gene            complement(1764..>2849)
                     /locus_tag="PLF56_12070"
     CDS             complement(1764..>2849)
                     /locus_tag="PLF56_12070"
                     /inference="COORDINATES: protein motif:HMM:NF024770.2"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_function: GO:0000976 - transcription cis-regulatory
                     region binding [Evidence IEA];
                     GO_function: GO:0003700 - DNA-binding transcription factor
                     activity [Evidence IEA];
                     GO_function: GO:0043565 - sequence-specific DNA binding
                     [Evidence IEA];
                     GO_process: GO:0006351 - DNA-templated transcription
                     [Evidence IEA];
                     GO_process: GO:0006355 - regulation of DNA-templated
                     transcription [Evidence IEA];
                     GO_process: GO:0045892 - negative regulation of
                     DNA-templated transcription [Evidence IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="LacI family DNA-binding transcriptional
                     regulator"
                     /protein_id="HOQ54334.1"
                     /translation="VARRSGVSTATVSYVLNRTTGQTISPETRARVLRAADELGYVQH
                     AAAQALARGASRIVVIDRGGIPHGDVAEHATAQLAESLRGRGFTPLLTPWNSGGSTDT
                     LLELARAMAPHAVVTVAPLDPDARAQLSAAGVQAFGTLFPGPLRLDDMIARAAHAQVE
                     FLAQQGHRGVLYMPATDPDLATLSALRGAAVSARASELGLRSQALDPTAIAGPLQRLT
                     DALPSVLSRTGLTAIAAYNDEVAMRVLAALHRLQLPVPERVAVIGVDDLPLAELTVPA
                     LTSVRYVPDESLLWQNRAERADALAWLTQPSQPPAPASHSGCPTEPGAPAVPTDRTPL
                     DDPDRSPSEAPHPVPTMCYQIVRRESA"
BASE COUNT          490 a         1000 c         1026 g          333 t
ORIGIN      
        1 gcgtcgcgcc atcaccgcgg ggtagagcgt gccgtagagc agaaacgcga cgaagccaaa
       61 ccccaggtag gccaggggca cgcccacagc caccagaaac agcgccggcg ccacctcgtt
      121 cagggccagt accaccagcc acgacccgac gtaaccgatc atgctgacgc ccaggccgac
      181 cgacagcaac cgccggatcg cgaacccctc gcgtcgcacc agcgtgacgc cggtggtgat
      241 caggaagacc ccaaggatca gcacggttgc cagcagcaag accagcagca ccagcagcac
      301 gacgcccgcg gctgtggagt cgaagcgtcg ctcggcagag tccagcagca atccgacgac
      361 ggcgagaacg gcccaagtca gtatggccag cagaaacagg ccggtgcgaa acgtgcgcgg
      421 gtcgttgcga tagctgagca ccagccccag cagtagcacc accacgccgc ccggcagttc
      481 gaccatcgct ctcgagccct ccggtgtcag gaatgggggc actcaggcta gcaagctcaa
      541 ctcgactcag gccgaggcgt cgttgctggc gggctgagcg ggatccggct ccagcggcct
      601 ggcgaccgga acgaccagca tcgccaacac cgacaccgcg ccgaccacca gcaatgcgcg
      661 cagcacgccg aaatggtcac caagaaagcc gagcagcggc gggccggcca agaaggcggt
      721 gtacccgatc gtcgacacca cactcagccg catcggcgcg cgagccggat cgtccgccgc
      781 cgccgacatg ccgaccggaa agccgaggct ggcgcccagg ccccagatcg cacacccgac
      841 gaaggcgacc cagacgttgc cgaacaccac catcaggcag ccgatgatcg cggcgccgaa
      901 caggattcgc agcaccgcga cccggccgta gcggtcgagc aggtgggtgc ccagaatgcg
      961 tcctgcggtc atgaagctga gaaagacggc cagggcgacg acgcccagcg cgttgtcgag
     1021 ttggtggccg tccacgaagg cgaccgccat ccagtcgttg gccgtgccct cggcgaacgc
     1081 cgcggcggtg accatcaggc cgatcagtag ggtgcggggc tccgtccacg ccgatcgttg
     1141 cggtgcggac ggggccgccg ccaccggtcc gccggcctcg tcgaaggccg ggaggaactg
     1201 cgtggtgccc caccagccga tcagggcggt gagcacagcg gcgccgctca ggtgcgccag
     1261 cagcggaacg ccgagccagg tgagcgcggc accgatcagg gcggccagaa ccgttccgcc
     1321 gctgaaggcc gcgtggaacc acggcatgat cgcccggtcg agggcctgct cgacgatgct
     1381 gccctccagg ttctgggcca cgtcccagac cccgctgccg aggcccgcca gaaacagccc
     1441 gggcatcgtc cagtacatcg ggagccctgc ctgcacggca gcggcggcca gcacgatgcc
     1501 gggggccatg gtggcgatgc ccagccggac cgtcaccacg gcgccgaatc ggtgcgcgat
     1561 ccgtccggcg gcgggaaggc cgaccagcgc gccgaccgac accgacagca gcagcacggc
     1621 cagcatgctg ggggtgagcg cgagcaactg tttgacgtcg ggcacccgcg acatccagct
     1681 ggcgaacgcg aagccgttgg cggcgaacac cagcagggtg ccggttcggg ctccggcaac
     1741 ggtgcgggaa acggtggaaa cggtcacgcg gattctctcc tgacgatctg gtagcacatg
     1801 gtggggaccg ggtgcggagc ctcactcggg gagcggtcgg ggtcgtcgag cggtgttctg
     1861 tcagtgggca cagccggcgc acccggctcg gtgggacagc ccgagtgtga ggccggggcg
     1921 gggggctgcg agggttgcgt caaccaggcc agggcatcgg cgcgttcggc ccggttctgc
     1981 cacagcaggg actcatccgg cacgtatcga acgctggtca gcgccggcac ggtcagctct
     2041 gccagcggca ggtcgtccac accgatcacg gcgacccgct cgggcaccgg cagttgcagc
     2101 cggtgcaggg cggccagcac gcgcatcgcc acctcgtcgt tgtaggcggc gatggccgtg
     2161 aggccggttc ggctgagaac ggacggcagc gcgtcggtca gccgctgcag tgggcccgcg
     2221 atcgcggtcg ggtcgagcgc ttggctgcgc aggccgagtt cggacgctct ggcgctcacc
     2281 gcggcgccgc gcagcgccga caaggtggcc aggtcggggt cggtggccgg catgtacagc
     2341 acgccgcggt ggccctgctg ggccaggaac tcgacctgcg cgtgcgccgc ccgcgcgatc
     2401 atgtcgtcca gccgcagcgg gcccgggaac aaggtgccga aggcctgcac ccccgccgcg
     2461 ctgagctgcg cgcgggcgtc cggatcgagg ggtgcgacgg tgaccaccgc gtgcggcgcc
     2521 atggcgcggg ccagttcgag cagggtgtcg gtcgagccgc cgctgttcca cggggtcagc
     2581 aggggagtga accctcgccc gcgcaggctc tcggcgagct gcgcggtggc gtgctcggcc
     2641 acgtcgccgt gcggaatgcc gcccctgtcg atcaccacga tccgggacgc cccgcgggcc
     2701 agggcctgcg cggcagcgtg ctggacgtag cccagttcgt ccgcggcccg gagcaccctc
     2761 gcgcgggtct cgggcgagat cgtctgcccc gtcgtgcggt tcagcacgta gctgacggtg
     2821 gccgtcgaga ccccactgcg gcgcgccac
//
LOCUS       DAOSUG010000418         2847 bp    DNA     linear   ENV 10-JUN-2024
DEFINITION  MAG TPA_asm: Micropruina sp. isolate AMR_MDS_2195
            NODE_14219_length_2847_cov_5.305516, whole genome shotgun sequence.
ACCESSION   DAOSUG010000418 DAOSUG010000000
VERSION     DAOSUG010000418.1
DBLINK      BioProject: PRJNA925477
            BioSample: SAMN32864262
            Sequence Read Archive: SRR4236673
KEYWORDS    WGS; ENV; Metagenome Assembled Genome; MAG; Third Party Data; TPA;
            TPA:assembly.
SOURCE      Micropruina sp. (wastewater metagenome)
  ORGANISM  Micropruina sp.
            Bacteria; Bacillati; Actinomycetota; Actinomycetes;
            Propionibacteriales; Nocardioidaceae; Micropruina.
REFERENCE   1  (bases 1 to 2847)
  AUTHORS   Abdulkadir,N., Saraiva,J.P., Zhang,J., Stolte,S., Gillor,O.,
            Harms,H. and Rocha,U.
  TITLE     Genome-centric analyses of 165 metagenomes show that mobile genetic
            elements are crucial for the transmission of antimicrobial
            resistance genes to pathogens in activated sludge and wastewater
  JOURNAL   Microbiol Spectr 12 (3), e0291823 (2024)
   PUBMED   38289113
REFERENCE   2  (bases 1 to 2847)
  AUTHORS   Abdulkadir,N.
  TITLE     Direct Submission
  JOURNAL   Submitted (24-MAR-2023) Environmental Microbiology, Helmholtz
            Center for Environmental Research -UFZ, Permoserstr 15, Leipzig
            04318, Germany
COMMENT     The annotation was added by the NCBI Prokaryotic Genome Annotation
            Pipeline (PGAP). Information about PGAP can be found here:
            https://www.ncbi.nlm.nih.gov/genome/annotation_prok/
            
            ##Genome-Assembly-Data-START##
            Assembly Method        :: SPAdes v. 3.11.1
            Genome Representation  :: Full
            Expected Final Version :: Yes
            Genome Coverage        :: Not Applicable
            Sequencing Technology  :: Illumina MiSeq
            ##Genome-Assembly-Data-END##
            
            ##Genome-Annotation-Data-START##
            Annotation Provider               :: NCBI
            Annotation Date                   :: 05/04/2023 04:57:10
            Annotation Pipeline               :: NCBI Prokaryotic Genome
                                                 Annotation Pipeline (PGAP)
            Annotation Method                 :: Best-placed reference protein
                                                 set; GeneMarkS-2+
            Annotation Software revision      :: 6.5
            Features Annotated                :: Gene; CDS; rRNA; tRNA; ncRNA
            Genes (total)                     :: 2,776
            CDSs (total)                      :: 2,750
            Genes (coding)                    :: 2,714
            CDSs (with protein)               :: 2,714
            Genes (RNA)                       :: 26
            tRNAs                             :: 24
            ncRNAs                            :: 2
            Pseudo Genes (total)              :: 36
            CDSs (without protein)            :: 36
            Pseudo Genes (ambiguous residues) :: 0 of 36
            Pseudo Genes (frameshifted)       :: 2 of 36
            Pseudo Genes (incomplete)         :: 32 of 36
            Pseudo Genes (internal stop)      :: 2 of 36
            ##Genome-Annotation-Data-END##
FEATURES             Location/Qualifiers
     source          1..2847
                     /organism="Micropruina sp."
                     /mol_type="genomic DNA"
                     /submitter_seqid="NODE_14219_length_2847_cov_5.305516"
                     /isolate="AMR_MDS_2195"
                     /isolation_source="Activated sludge of municipal
                     wastewater treatment plants"
                     /db_xref="taxon:2737536"
                     /environmental_sample
                     /geo_loc_name="Canada"
                     /lat_lon="49.25 N 123.1 W"
                     /collection_date="2015-06-16"
                     /metagenome_source="wastewater metagenome"
                     /note="metagenomic"
     gene            complement(131..1018)
                     /gene="gnd"
                     /locus_tag="PLF56_12075"
     CDS             complement(131..1018)
                     /gene="gnd"
                     /locus_tag="PLF56_12075"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_008355537.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_function: GO:0004616 - phosphogluconate dehydrogenase
                     (decarboxylating) activity [Evidence IEA];
                     GO_process: GO:0006098 - pentose-phosphate shunt [Evidence
                     IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="decarboxylating 6-phosphogluconate
                     dehydrogenase"
                     /protein_id="HOQ54335.1"
                     /translation="MQLGLVGLGKMGHNMRERMRQAGLTVVGYDRNPDVSDTASLAEL
                     AERLSGQGPRVIWVMLPIQVVDQTIDDLKDLLAEGDLVIDGGNTKWTDDDVHARSLAE
                     KGIHFLDCGVSGGVWGLKNGYALMVGGDAADVALAQPIFDALKPQGEFGFVHAGQVGA
                     GHFVKMVHNGIEYGLLQAYAEGWELIEAAGSVTNVTEVFNSWREGTVIRSWLLDLMVL
                     ALQDDPHLDRIEGYAADSGEGRWTVNAAVNLGVPVPTMAAALFARFTSQQADSPSMKM
                     VAAVRNQFGGHAMKTEPNA"
     gene            complement(1121..2281)
                     /gene="dnaN"
                     /locus_tag="PLF56_12080"
     CDS             complement(1121..2281)
                     /gene="dnaN"
                     /locus_tag="PLF56_12080"
                     /EC_number="2.7.7.7"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_002512706.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_component: GO:0009360 - DNA polymerase III complex
                     [Evidence IEA];
                     GO_function: GO:0003887 - DNA-directed DNA polymerase
                     activity [Evidence IEA];
                     GO_process: GO:0006260 - DNA replication [Evidence IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="DNA polymerase III subunit beta"
                     /protein_id="HOQ54336.1"
                     /translation="MKIRLDRDVLAEAVAWTARSLPTRPSVPILAGLLIKASSGQVVM
                     SSFDYETSAQITVKAEVTDDGVALVSGRLLADIARSLPNKPVDLTADASRMELVCGSA
                     RFTLQTLPVDDYPALPSMPEATGTVTSDEFARAVSQVVVAAGRDELLPVFTGVRMEIE
                     GDTLSLLATDRYRMALKEISWNPGTARTDGAALVPARVLGETAKSMTSGEVVTLSLAA
                     TGSGDGLIGFEGDGAAGVREITTRLLDGEFPKVRHLMNVQATLTVRANTAEVIAAVKR
                     VGLVAERNTSLRMIIGDQAITLEAATGDQAQAVEALEAVVHDETGNGPSLSAAGFNPH
                     YLSDALAALDAPYVHFAFTAPGKPCLLTALNELDGEPLGDYRHVIMLMRLPA"
BASE COUNT          467 a          947 c          949 g          484 t
ORIGIN      
        1 ggtgacgaac acgccgtcac cctacgacac cgggcgaggc gcggcggctg acgcaacatg
       61 tgcagcgggt tgagtgcccg tccgggcgag agggtgaacc gggtgtgtga actgagttcg
      121 accagcggcc tcaggcgttc ggctccgtct tcatcgcgtg tccgccgaac tggttgcgca
      181 ccgctgcgac catcttcatc gatggcgagt ccgcctgctg cgaggtgaac cgggcgaaca
      241 gtgccgccgc catggtcggc accggaaccc ccaggttcac ggctgcgttg accgtccagc
      301 gtccctcgcc cgagtcggcc gcgtagccct cgatccggtc cagatgcgga tcgtcctgca
      361 gcgccagcac catcaggtcg agcagccacg agcgaatcac cgtgccctcg cgccaggagt
      421 tgaagacctc ggtcacgttg gtcacgctgc cggccgcctc gatcaactcc caaccctcgg
      481 cgtaggcctg cagcaacccg tactcgatgc cgttgtgcac catcttcacg aaatgcccgg
      541 cccccacctg gccggcatgc acgaagccga actcgccctg aggcttcaag gcgtcgaaga
      601 tcggttgggc gagtgcgacg tccgccgcgt cgccgccgac catcagcgcg tagccgttct
      661 tgagccccca gacgcccccg gagacaccgc agtcgagaaa gtggatgccc ttctcggcca
      721 gcgagcgggc gtgcacgtca tcatcggtcc acttggtgtt gccgccgtcg atcaccaggt
      781 cgccctcggc gagcaggtcc ttcagatcat cgatcgtctg atcgaccacc tggatcggca
      841 gcatcaccca gatcacacgg gggccctggc cactgagccg ttcggccaac tcggccagcg
      901 aggcggtgtc gctgacgtca gggttgcggt cgtagcccac cacggtgagg ccggcctgac
      961 gcatgcgctc gcgcatgttg tggcccatct tgcccaggcc aaccagtccg agttgcatgc
     1021 ctacctccaa gaagacgcgt tgatgctgac gaggcgacgc taccgcgtcg tgtactgccc
     1081 gcacccctgc aactaaggct gatctcagtg tggaccgggc tcaggccggc aggcgcatca
     1141 gcatgatcac gtggcggtag tcgccgagcg gttcgccgtc gagttcgttg agcgcggtca
     1201 gcaggcaggg cttgccgggc gcggtgaaag cgaagtgcac gtacggggcg tccagggcgg
     1261 ccagggcgtc cgaaaggtaa tgcgggttga agccggcggc gctcagcgag gggccgttgc
     1321 cggtctcgtc gtgcacgacc gcctccaggg cctcgacagc ctgcgcctgg tcgccggtgg
     1381 cggcctccag cgtgatcgcc tggtcgccga tgatcatgcg cagcgaggtg ttgcgctcgg
     1441 ccaccagtcc gacgcgcttg actgcggcga tcacctcggc ggtgttggcg cgcacggtca
     1501 gggtggcctg cacgttcatc aggtgccgca ccttgggaaa ctcgccgtcg agcagccggg
     1561 tggtgatctc gcgcaccccg gcggccccgt cgccctcgaa cccgatcagg ccgtcgccac
     1621 taccggtggc ggccaggctg agcgtcacca cctcgccgga ggtcatcgac ttggccgtct
     1681 cgcccagcac ccgggccggc accagggcgg ccccgtcggt acgggccgtg cccgggttcc
     1741 acgagatctc tttgagcgcc atgcggtagc ggtcggtggc gagcaatgac agggtgtcgc
     1801 cctcgatctc catgcggacg ccggtgaaca cgggcagcaa ctcgtcgcgt ccggcggcca
     1861 ccaccacttg ggacacggcg cgggcgaact cgtcgctggt cacggtgccg gtggcctcgg
     1921 gcatgctcgg caaagccggg tagtcgtcga ccggaagggt ctgcagggtg aacctggcac
     1981 tgccacagac caactccatg cgcgaggcgt ccgcggtcag gtcgacaggc ttgttgggta
     2041 gcgaacgcgc gatgtcggcg agcagccggc ccgagaccag cgcaaccccg tcgtcggtga
     2101 cctcggcctt gacggtgatc tgggcggagg tctcgtagtc gaagctcgac atcaccacct
     2161 gaccgctcga ggccttgatc aacaggccgg cgagaatcgg cacactcggc ctggtgggca
     2221 ggctgcgcgc agtccaggcg accgcttcgg cgagtacatc gcgatcaaga cggatcttca
     2281 cgtggctccc ttgctcatac ttccggggat cgatcatatc cgcagcagcc agactgcgat
     2341 cggcatccac accatgggta ccggcgctac tttgagtgat ctttgacgag taacaattct
     2401 tcgtagtcat agctggtgtg aattctgggg acaacggacg aagtcgctgc tcagagcccg
     2461 gttgcggctc tggacgaggc tgtgcacaag tcgaaatcac acgggtagta gttgcggacg
     2521 ggcgcggccc gcgtggcgtt gtccacagcg aaggacgaca agttcctgta gttctgcacc
     2581 gtcgcagcga cagcctgtgt gggacggagg cggccctcga caccgcgtcc atgtgtggtc
     2641 tcgaccagcc cgaccgaacg gatcgcgcgt tgatcagcgc ttcccgaaac gtcggcgggt
     2701 gacgtcgctt gacacaacgt ggggcatcag gctcgacggc ggcgctcgac cgatcagcgc
     2761 ttggcggcgc gttgcttgat ccggttggtc aactcggcga cctggttgaa cactttgcgg
     2821 tcctcgccca tcaggtggcg gatcttc
//
LOCUS       DAOSUG010000419         2843 bp    DNA     linear   ENV 10-JUN-2024
DEFINITION  MAG TPA_asm: Micropruina sp. isolate AMR_MDS_2195
            NODE_14245_length_2843_cov_5.330703, whole genome shotgun sequence.
ACCESSION   DAOSUG010000419 DAOSUG010000000
VERSION     DAOSUG010000419.1
DBLINK      BioProject: PRJNA925477
            BioSample: SAMN32864262
            Sequence Read Archive: SRR4236673
KEYWORDS    WGS; ENV; Metagenome Assembled Genome; MAG; Third Party Data; TPA;
            TPA:assembly.
SOURCE      Micropruina sp. (wastewater metagenome)
  ORGANISM  Micropruina sp.
            Bacteria; Bacillati; Actinomycetota; Actinomycetes;
            Propionibacteriales; Nocardioidaceae; Micropruina.
REFERENCE   1  (bases 1 to 2843)
  AUTHORS   Abdulkadir,N., Saraiva,J.P., Zhang,J., Stolte,S., Gillor,O.,
            Harms,H. and Rocha,U.
  TITLE     Genome-centric analyses of 165 metagenomes show that mobile genetic
            elements are crucial for the transmission of antimicrobial
            resistance genes to pathogens in activated sludge and wastewater
  JOURNAL   Microbiol Spectr 12 (3), e0291823 (2024)
   PUBMED   38289113
REFERENCE   2  (bases 1 to 2843)
  AUTHORS   Abdulkadir,N.
  TITLE     Direct Submission
  JOURNAL   Submitted (24-MAR-2023) Environmental Microbiology, Helmholtz
            Center for Environmental Research -UFZ, Permoserstr 15, Leipzig
            04318, Germany
COMMENT     The annotation was added by the NCBI Prokaryotic Genome Annotation
            Pipeline (PGAP). Information about PGAP can be found here:
            https://www.ncbi.nlm.nih.gov/genome/annotation_prok/
            
            ##Genome-Assembly-Data-START##
            Assembly Method        :: SPAdes v. 3.11.1
            Genome Representation  :: Full
            Expected Final Version :: Yes
            Genome Coverage        :: Not Applicable
            Sequencing Technology  :: Illumina MiSeq
            ##Genome-Assembly-Data-END##
            
            ##Genome-Annotation-Data-START##
            Annotation Provider               :: NCBI
            Annotation Date                   :: 05/04/2023 04:57:10
            Annotation Pipeline               :: NCBI Prokaryotic Genome
                                                 Annotation Pipeline (PGAP)
            Annotation Method                 :: Best-placed reference protein
                                                 set; GeneMarkS-2+
            Annotation Software revision      :: 6.5
            Features Annotated                :: Gene; CDS; rRNA; tRNA; ncRNA
            Genes (total)                     :: 2,776
            CDSs (total)                      :: 2,750
            Genes (coding)                    :: 2,714
            CDSs (with protein)               :: 2,714
            Genes (RNA)                       :: 26
            tRNAs                             :: 24
            ncRNAs                            :: 2
            Pseudo Genes (total)              :: 36
            CDSs (without protein)            :: 36
            Pseudo Genes (ambiguous residues) :: 0 of 36
            Pseudo Genes (frameshifted)       :: 2 of 36
            Pseudo Genes (incomplete)         :: 32 of 36
            Pseudo Genes (internal stop)      :: 2 of 36
            ##Genome-Annotation-Data-END##
FEATURES             Location/Qualifiers
     source          1..2843
                     /organism="Micropruina sp."
                     /mol_type="genomic DNA"
                     /submitter_seqid="NODE_14245_length_2843_cov_5.330703"
                     /isolate="AMR_MDS_2195"
                     /isolation_source="Activated sludge of municipal
                     wastewater treatment plants"
                     /db_xref="taxon:2737536"
                     /environmental_sample
                     /geo_loc_name="Canada"
                     /lat_lon="49.25 N 123.1 W"
                     /collection_date="2015-06-16"
                     /metagenome_source="wastewater metagenome"
                     /note="metagenomic"
     gene            complement(<1..799)
                     /locus_tag="PLF56_12085"
     CDS             complement(<1..799)
                     /locus_tag="PLF56_12085"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_015070526.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_function: GO:0004553 - hydrolase activity, hydrolyzing
                     O-glycosyl compounds [Evidence IEA];
                     GO_process: GO:0005975 - carbohydrate metabolic process
                     [Evidence IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="family 1 glycosylhydrolase"
                     /protein_id="HOQ54337.1"
                     /translation="MSTVKTVDPEISPDPALSPQVLRFPAGFHWGTATSSFQIEGAAE
                     LRAPSIWDDFCRVPGTIADGSDGLVAVDHYHRYREDVALMASLGMDTYRFSVSWPRVL
                     DPDGSTNAEGLGFYDALVDELCQAGIRPWLTLYHWDLPSYLPGGWLNRDTASAFVDYA
                     LRVHAVLGDRVRVWTTLNEPWCASFLSFAGGAHAPGHTVPAEAYTAGHHLLLGHGWAT
                     QALRAADPSATLGLTLNFTPAHPAEPTDPDDLSVVRRIDGTTHRFFTD"
     gene            complement(796..1704)
                     /locus_tag="PLF56_12090"
     CDS             complement(796..1704)
                     /locus_tag="PLF56_12090"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_015070525.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_process: GO:0008643 - carbohydrate transport [Evidence
                     IEA];
                     GO_process: GO:0055085 - transmembrane transport [Evidence
                     IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="carbohydrate ABC transporter permease"
                     /protein_id="HOQ54338.1"
                     /translation="MSSVAMADQLAGKGAARAAARRKHRMEGGVRSPGWVTYTLLGLI
                     VCISILPLYYAFLLSTSTAADIAQRPIPSLIPQGHFIENVSRVLNAGIGFWQALMNSI
                     IVSTITALSVVFFSTLAGFSFSKLRFRGRNGLLAFVIGTMAVPTQLGVIPLFIVMSRL
                     DWIGKLQAVIVPGLVTAFGVFWMTQYLSEALPFELIEAARIDGCSMIRTFWHVALPAA
                     RPAAAMLALFTFVGSWTNFFWPFIALGSTNPTLPVALQLLQASYFKDLALIMAGVVLA
                     TIPLLVLFVFAGRQLVSGIMAGAVKG"
     gene            complement(1706..2635)
                     /locus_tag="PLF56_12095"
     CDS             complement(1706..2635)
                     /locus_tag="PLF56_12095"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_012922828.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_function: GO:0042626 - ATPase-coupled transmembrane
                     transporter activity [Evidence IEA];
                     GO_function: GO:0140359 - ABC-type transporter activity
                     [Evidence IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="sugar ABC transporter permease"
                     /protein_id="HOQ54339.1"
                     /translation="MTWQQRLSRFDVRAAPYLYIAPFFLLFALVGMFPLLYTGWVALN
                     RWSLVTGNQGFIGMGNFAYVIDQPRFWDAVVNTFSIFVLSSVPQLILALFIAAMLDAN
                     LRAKTFWRMGVLVPYVVAPVAVSLVFGQLFADQSGVINTLLGTVGIDPIGWHADRFAS
                     HAAIATMVNFRWTGYNALIMLAAMQAIPRDVIEAAVVDGANRWRTFLHVTVPLLRPTL
                     IFVVITSTIGGLQIFDEPRLFDTFGLGGPDREWMTVTMYLYELGWAGQRNLGRAAAVA
                     WLLFLIIVLFALLNYAITRRIASTEARPKRKGR"
BASE COUNT          544 a          879 c         1026 g          394 t
ORIGIN      
        1 ggtcggtgaa gaagcggtgt gtcgtgccgt cgatgcgtcg caccacgctc aggtcgtccg
       61 gatccgtggg ctcggcgggg tgggcggggg tgaagttgag ggtcaacccc aacgtcgcgg
      121 acggatccgc ggcccgcagt gcctgcgtgg cccacccgtg gcccagcagc aggtggtggc
      181 ccgcggtgta ggcctcggcc ggcacggtgt gacccggtgc gtgggcgccg ccggcgaacg
      241 acaggaacga cgcgcaccac ggttcgttga gggtcgtcca gacgcgtacc cggtcaccga
      301 gcacggcatg cacccgcagg gcgtagtcga cgaacgcgga cgcggtgtca cggttcagcc
      361 agccgcccgg caggtagctg ggcagatccc agtggtacag cgtcagccac ggccggatgc
      421 ccgcctggca cagctcgtcc accagggcgt cgtagaagcc gagcccctcg gcgttggtgc
      481 tgccgtcggg gtccagaacc cgcggccacg acaccgagaa ccggtaggtg tccatgccga
      541 gtgaggccat cagggcgacg tcctcgcggt agcggtggta gtgatcgacg gcgacgagcc
      601 cgtccgaacc gtcggcgatg gtgcccggca cccggcagaa gtcgtcccag atggacgggg
      661 cgcgcagttc ggccgcgccc tcgatctgaa aggacgacgt ggcggtgccc caatggaagc
      721 cggccggaaa ccgcaggacc tgcggcgaaa gcgccggatc gggcgagatt tcggggtcga
      781 cagtcttcac ggtgctcatc ccttcactgc tcccgccatg atgcccgaga ccagctggcg
      841 tccggcgaag acgaacaaca cgagcaacgg aatagtggcc agcaccacac cggccatgat
      901 cagtgccagg tccttgaagt acgacgcctg cagcagttgg agtgccaccg gcagggtggg
      961 gttggtcgaa cccagcgcga tgaagggcca gaagaagttc gtccacgagc cgacgaaggt
     1021 gaacagcgcc agcatcgccg ccgccggacg tgccgcgggc agggcgacgt gccagaacgt
     1081 ccggatcatc gagcagccgt cgatgcgcgc tgcctcgatc aattcgaacg gcagcgcctc
     1141 tgacagatac tgcgtcatcc agaacacgcc gaaagcggtg accaggccgg gcacgatgac
     1201 cgcttgcagc ttgccgatcc agtccagccg tgacatcaca atgaacagcg gaatgacgcc
     1261 gagttgggtg ggtaccgcca tggtgccgat cacgaaggcc agcaacccgt tgcgaccgcg
     1321 gaaccgcagc ttcgagaacg agaacccggc caaggtcgag aagaacacca ccgacagggc
     1381 ggtgatcgtc gacacaatga tgctgttcat cagcgcctgc cagaacccga tgcccgcatt
     1441 cagcacccgc gacacgttct cgatgaagtg gccttgcgga atcagggacg ggatgggccg
     1501 ctgcgcgatg tcggccgcgg tcgaggtgga cagtagaaag gcgtagtaga gcggcaggat
     1561 cgagatgcag acgatcagac ccaacaaggt gtaggtgacc cagccggggc tgcggacgcc
     1621 gccctccatg cggtgcttgc gacgggctgc ggcccgcgcg gcccccttgc cggccaactg
     1681 atcggccatg gcgacggaac tcatgtcagc gaccctttcg cttgggacgg gcctcggtgc
     1741 tggcgatccg tcgggtgatg gcgtagttga gtagggcgaa cagcacgatg atcaggaaca
     1801 acagccacgc cacggcggcg gcccggccca ggttgcgttg cccggcccag ccgagctcgt
     1861 agaggtacat ggtgaccgtc atccactcac ggtccgggcc gcccaggccg aaggtgtcga
     1921 acaggcgcgg ctcgtcgaag atctgcagcc caccgatggt ggaggtgatc accacgaaga
     1981 tcagggtcgg tcgtagcagt ggcacggtga cgtgcagaaa ggtgcgccaa cgattggcgc
     2041 cgtcgaccac agcggcctcg atcacgtcgc gaggaatggc ctgcatcgcg gccagcatga
     2101 tcagcgcgtt gtagccggtc cagcgaaagt tgaccatggt ggcgatcgcg gcgtgcgatg
     2161 cgaaccggtc ggcgtgccag ccgatcggat cgatgccgac cgtgcccagc agcgtgttga
     2221 tcacgcccga ctggtcggcg aacagttgcc cgaacaccag cgagactgcc accggcgcga
     2281 ccacgtacgg caccagcacg cccatgcgcc agaacgtctt ggcgcgcagg ttggcatcga
     2341 gcatggcggc gatgaacagg gccagaatca gctgtggcac cgacgacagc acgaagatgc
     2401 tgaaggtgtt gaccaccgcg tcccagaagc ggggctggtc gatcacatag gcgaagttgc
     2461 ccatgccgat gaagccctga ttgccggtga ccaggctcca ccggttgagc gccacccagc
     2521 cggtgtacag caggggaaac atgccgacga gggcgaagag caggaagaac ggggcgatgt
     2581 agaggtaggg cgcggcgcgc acgtcgaagc ggctgagtcg ctgttgccag gtcaccggat
     2641 gtctccattt cggctggggc ccgatcgagc gtgggcgcgg tgagcacacg aggtgcagga
     2701 ctgggtggtg ggctgcgcag catcgagcag ccggggccgg tgtacggcgg gcgagggtgt
     2761 gcggcagggg cggaggacac gcggccggtg tgcggcaagc gcgggtgtcc ggcgactgcg
     2821 tggtgccggc gggaccctgg acg
//
LOCUS       DAOSUG010000420         2840 bp    DNA     linear   ENV 10-JUN-2024
DEFINITION  MAG TPA_asm: Micropruina sp. isolate AMR_MDS_2195
            NODE_14272_length_2840_cov_4.984560, whole genome shotgun sequence.
ACCESSION   DAOSUG010000420 DAOSUG010000000
VERSION     DAOSUG010000420.1
DBLINK      BioProject: PRJNA925477
            BioSample: SAMN32864262
            Sequence Read Archive: SRR4236673
KEYWORDS    WGS; ENV; Metagenome Assembled Genome; MAG; Third Party Data; TPA;
            TPA:assembly.
SOURCE      Micropruina sp. (wastewater metagenome)
  ORGANISM  Micropruina sp.
            Bacteria; Bacillati; Actinomycetota; Actinomycetes;
            Propionibacteriales; Nocardioidaceae; Micropruina.
REFERENCE   1  (bases 1 to 2840)
  AUTHORS   Abdulkadir,N., Saraiva,J.P., Zhang,J., Stolte,S., Gillor,O.,
            Harms,H. and Rocha,U.
  TITLE     Genome-centric analyses of 165 metagenomes show that mobile genetic
            elements are crucial for the transmission of antimicrobial
            resistance genes to pathogens in activated sludge and wastewater
  JOURNAL   Microbiol Spectr 12 (3), e0291823 (2024)
   PUBMED   38289113
REFERENCE   2  (bases 1 to 2840)
  AUTHORS   Abdulkadir,N.
  TITLE     Direct Submission
  JOURNAL   Submitted (24-MAR-2023) Environmental Microbiology, Helmholtz
            Center for Environmental Research -UFZ, Permoserstr 15, Leipzig
            04318, Germany
COMMENT     The annotation was added by the NCBI Prokaryotic Genome Annotation
            Pipeline (PGAP). Information about PGAP can be found here:
            https://www.ncbi.nlm.nih.gov/genome/annotation_prok/
            
            ##Genome-Assembly-Data-START##
            Assembly Method        :: SPAdes v. 3.11.1
            Genome Representation  :: Full
            Expected Final Version :: Yes
            Genome Coverage        :: Not Applicable
            Sequencing Technology  :: Illumina MiSeq
            ##Genome-Assembly-Data-END##
            
            ##Genome-Annotation-Data-START##
            Annotation Provider               :: NCBI
            Annotation Date                   :: 05/04/2023 04:57:10
            Annotation Pipeline               :: NCBI Prokaryotic Genome
                                                 Annotation Pipeline (PGAP)
            Annotation Method                 :: Best-placed reference protein
                                                 set; GeneMarkS-2+
            Annotation Software revision      :: 6.5
            Features Annotated                :: Gene; CDS; rRNA; tRNA; ncRNA
            Genes (total)                     :: 2,776
            CDSs (total)                      :: 2,750
            Genes (coding)                    :: 2,714
            CDSs (with protein)               :: 2,714
            Genes (RNA)                       :: 26
            tRNAs                             :: 24
            ncRNAs                            :: 2
            Pseudo Genes (total)              :: 36
            CDSs (without protein)            :: 36
            Pseudo Genes (ambiguous residues) :: 0 of 36
            Pseudo Genes (frameshifted)       :: 2 of 36
            Pseudo Genes (incomplete)         :: 32 of 36
            Pseudo Genes (internal stop)      :: 2 of 36
            ##Genome-Annotation-Data-END##
FEATURES             Location/Qualifiers
     source          1..2840
                     /organism="Micropruina sp."
                     /mol_type="genomic DNA"
                     /submitter_seqid="NODE_14272_length_2840_cov_4.984560"
                     /isolate="AMR_MDS_2195"
                     /isolation_source="Activated sludge of municipal
                     wastewater treatment plants"
                     /db_xref="taxon:2737536"
                     /environmental_sample
                     /geo_loc_name="Canada"
                     /lat_lon="49.25 N 123.1 W"
                     /collection_date="2015-06-16"
                     /metagenome_source="wastewater metagenome"
                     /note="metagenomic"
     gene            <1..111
                     /locus_tag="PLF56_12100"
     CDS             <1..111
                     /locus_tag="PLF56_12100"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_003866459.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="exodeoxyribonuclease III"
                     /protein_id="HOQ54340.1"
                     /translation="ASPGLAMRAIAAGTDREPSYEQRMSDHSPVVVDYAW"
     gene            617..>2840
                     /locus_tag="PLF56_12105"
     CDS             617..>2840
                     /locus_tag="PLF56_12105"
                     /inference="COORDINATES: ab initio
                     prediction:GeneMarkS-2+"
                     /note="Derived by automated computational analysis using
                     gene prediction method: GeneMarkS-2+."
                     /codon_start=1
                     /transl_table=11
                     /product="hypothetical protein"
                     /protein_id="HOQ54341.1"
                     /translation="MSITYQFDLSHLDPDRTYTLHKRGRVYPLRRHTRASLAEALGDD
                     RRLQAARQRITHYVSGVATSPSDGVVIQAVRTPVTVDGPKGPGTQTYNQLVTFAISIP
                     GADSPLNDAEDLACALLFMHNNLANLSAQQDFTVPTYILGTCIRPNAGDLAQQLSELP
                     LQGESWISDPYAVMTSATQHLLYPSPPSPDALANDPLLWREGRDPLIERLLRMRNQPT
                     DSGSQPGDWVYSQRLNVDVLQPALSGSLGAALRAAKNAEQLEGITWNTQDAVASSDYA
                     ASTEPPRSRRATGPAATAGADDVTWTVTNLSPGSGLDVDASSLKVDMTKDTDNPDYAG
                     QLTLNCTNNWLRHLSAYVQFLKYSGEGSSQTLVPIDLGASDTEVKVADPSEPGHYIYE
                     YVNTKWPGDFCNVKDPLWSGMGFIEPDHTKKFLTWVSPTGTICGIPVPAAPTTLIVDY
                     MPKDTNVVRIMWGGMGRGPYDQDVCALGISFTAVFELALPMILLIYDAATVDSAALTE
                     TVWNHKGTMFNLAKTIVSVVMNTKLNADTMIQQDAEAIGKFLAETLLPDLLQTGCAYG
                     VILLAQIAVGAVVESIPFLDIASLIFNGAITVAQLSQTIVEVVDSPFVFTTDIARTFD
                     LTLTIDPDSTMHEFPPEAIGGTYQVAIAFSTNATPAVTTRQMPATTWSDPLQLSFSEL
                     PAGGQIQLNVYMYSPTGWQAGQGQSAWIDAKGDGTGPNPSTRTVEVTVTTPVVSVMAT
                     P"
BASE COUNT          553 a         1020 c          835 g          432 t
ORIGIN      
        1 gcctcacccg gcctcgccat gcgagccatc gccgccggga ccgatcgcga gccgagctac
       61 gaacagcgga tgagcgacca ctcccccgtc gtcgtcgact acgcgtggtg aggttgccgc
      121 gccacatctc gaacgcgagc aaagaatgct acgcattcgc cggcaggggc tagacaaagc
      181 gtcgtgggtt cacaacgatt cgtcatttca accgaagtca ccaggccaac caatagggac
      241 ctgaagcaca ttttccgggc gacacgccag cggacagcgt cgaatagagc agggcaattg
      301 tgccacctcg ccgactgtgc ggggtcgccg tgccatacgg catccctgtg ccgcgtgact
      361 actggaggcg catgcactgg cccgcgacaa ctgcacggac caggactcaa cggcctgatc
      421 atgggcgctc tattgacgcg ccccggatta tgggagcatg ctcaacccgc gcctttcttc
      481 aagcctgaag aagtgccaag aatgctcacc cacccctcga gcattgctaa agccgacgaa
      541 ccgtccgttg ccgatcccac cacccctgcc gcgatgggcg ctttcagtcg cggcataacc
      601 ggaatgcgat gaccagatga gcatcacgta ccagttcgac ctgtcccatc tcgaccccga
      661 ccgcacctac accctgcaca agcgcggacg ggtgtacccg ctgcgccggc acacccgagc
      721 cagcctggcc gaggccctgg gcgacgaccg caggctccaa gcggcgaggc agcggatcac
      781 ccactacgtc agcggggtcg ccaccagccc atccgacggg gtggtgatcc aggcggtgcg
      841 cacccccgtc accgtcgacg gtcccaaagg gcccggcacc cagacctaca accagctggt
      901 cacctttgcc atctcgatcc cgggtgccga cagcccgttg aacgacgccg aggatctcgc
      961 ctgtgcgctg ctcttcatgc acaacaacct ggccaacctg tcggcgcagc aggactttac
     1021 ggtgcccacc tacattctcg gcacctgcat ccggccgaac gcgggcgatc tggcccagca
     1081 gctgtcggaa ctaccactgc agggtgagag ctggatcagc gacccgtacg ccgtgatgac
     1141 cagtgcaacc cagcatctgc tctatccctc gcccccctca ccggacgccc tcgccaacga
     1201 cccgctgctg tggcgcgaag gccgagatcc cctgatcgaa cgactgctgc ggatgcgcaa
     1261 ccagcccacc gactccggct cgcagccggg cgactgggtg tactcccagc ggttgaacgt
     1321 ggacgtgctg cagccggccc tgtcgggctc gctcggtgcg gcgttgcgcg ccgccaagaa
     1381 cgccgaacag cttgagggca tcacctggaa cactcaggat gcggtcgcct ccagcgacta
     1441 cgcagccagc accgagcccc cgcggtcacg ccgcgcaacg ggaccggccg ccaccgccgg
     1501 tgcggacgac gtcacctgga cggtgaccaa cctctccccc ggcagcggac tggacgtgga
     1561 cgcgtcctcg ctgaaggtcg acatgaccaa ggacaccgac aaccccgact acgcgggcca
     1621 gctcaccctg aactgcacca acaactggct gcggcacctg tcggcctacg tgcagttcct
     1681 gaagtactcc ggcgagggtt cgtcgcagac cctggtgccg atcgacctgg gcgccagcga
     1741 caccgaggtg aaggttgccg acccgagcga gccggggcac tacatctatg agtacgtgaa
     1801 caccaagtgg ccgggcgact tctgcaacgt caaggacccg ctctggtcag gcatgggctt
     1861 catcgaaccg gaccacacca agaagttcct cacctgggtg agccccaccg gaacgatctg
     1921 cggcatcccg gtgcccgcgg cacccaccac gctgatcgtc gactacatgc caaaggacac
     1981 caacgtcgtc cggatcatgt ggggcggaat gggtcgcggc ccctacgacc aggacgtctg
     2041 cgcgctgggc atttcgttca ccgcggtgtt cgagttggca ctaccgatga ttctgctgat
     2101 ctacgacgcg gccaccgtcg acagcgcagc gctcaccgag acggtgtgga accacaaggg
     2161 cacgatgttc aacctggcca agaccatcgt gagcgtggtg atgaacacca agctgaacgc
     2221 cgacacgatg attcagcagg acgccgaggc gatcggcaag tttctggccg aaaccctgct
     2281 gcccgacctg ctgcagaccg gctgtgccta cggggtgatc ctgctggccc agatcgccgt
     2341 cggcgccgtg gtcgagtcga ttccgttcct cgacatcgcg tcgttgatct tcaacggcgc
     2401 catcaccgtc gcccagctca gccagaccat cgtcgaagtg gtggattcgc cgttcgtgtt
     2461 caccaccgat atcgcccgca ccttcgacct caccctcacc atcgaccccg actccacgat
     2521 gcacgagttt ccgccggagg cgatcggcgg cacctaccag gtcgccatcg ccttctcgac
     2581 caacgccacc ccggcagtga ccacgagaca gatgccggcc accacgtgga gcgatccgct
     2641 gcagctgagc ttttccgaac tgcccgccgg cgggcagatc cagctgaacg tctacatgta
     2701 ctcaccgacc ggctggcagg ccgggcaggg ccagagcgcc tggatcgacg ccaagggcga
     2761 cggcaccggc ccgaacccgt ccaccagaac ggtcgaggtc acggtgacca cgccggtggt
     2821 atcggtgatg gcgacgccgc
//
LOCUS       DAOSUG010000421         2825 bp    DNA     linear   ENV 10-JUN-2024
DEFINITION  MAG TPA_asm: Micropruina sp. isolate AMR_MDS_2195
            NODE_14348_length_2825_cov_3.097473, whole genome shotgun sequence.
ACCESSION   DAOSUG010000421 DAOSUG010000000
VERSION     DAOSUG010000421.1
DBLINK      BioProject: PRJNA925477
            BioSample: SAMN32864262
            Sequence Read Archive: SRR4236673
KEYWORDS    WGS; ENV; Metagenome Assembled Genome; MAG; Third Party Data; TPA;
            TPA:assembly.
SOURCE      Micropruina sp. (wastewater metagenome)
  ORGANISM  Micropruina sp.
            Bacteria; Bacillati; Actinomycetota; Actinomycetes;
            Propionibacteriales; Nocardioidaceae; Micropruina.
REFERENCE   1  (bases 1 to 2825)
  AUTHORS   Abdulkadir,N., Saraiva,J.P., Zhang,J., Stolte,S., Gillor,O.,
            Harms,H. and Rocha,U.
  TITLE     Genome-centric analyses of 165 metagenomes show that mobile genetic
            elements are crucial for the transmission of antimicrobial
            resistance genes to pathogens in activated sludge and wastewater
  JOURNAL   Microbiol Spectr 12 (3), e0291823 (2024)
   PUBMED   38289113
REFERENCE   2  (bases 1 to 2825)
  AUTHORS   Abdulkadir,N.
  TITLE     Direct Submission
  JOURNAL   Submitted (24-MAR-2023) Environmental Microbiology, Helmholtz
            Center for Environmental Research -UFZ, Permoserstr 15, Leipzig
            04318, Germany
COMMENT     The annotation was added by the NCBI Prokaryotic Genome Annotation
            Pipeline (PGAP). Information about PGAP can be found here:
            https://www.ncbi.nlm.nih.gov/genome/annotation_prok/
            
            ##Genome-Assembly-Data-START##
            Assembly Method        :: SPAdes v. 3.11.1
            Genome Representation  :: Full
            Expected Final Version :: Yes
            Genome Coverage        :: Not Applicable
            Sequencing Technology  :: Illumina MiSeq
            ##Genome-Assembly-Data-END##
            
            ##Genome-Annotation-Data-START##
            Annotation Provider               :: NCBI
            Annotation Date                   :: 05/04/2023 04:57:10
            Annotation Pipeline               :: NCBI Prokaryotic Genome
                                                 Annotation Pipeline (PGAP)
            Annotation Method                 :: Best-placed reference protein
                                                 set; GeneMarkS-2+
            Annotation Software revision      :: 6.5
            Features Annotated                :: Gene; CDS; rRNA; tRNA; ncRNA
            Genes (total)                     :: 2,776
            CDSs (total)                      :: 2,750
            Genes (coding)                    :: 2,714
            CDSs (with protein)               :: 2,714
            Genes (RNA)                       :: 26
            tRNAs                             :: 24
            ncRNAs                            :: 2
            Pseudo Genes (total)              :: 36
            CDSs (without protein)            :: 36
            Pseudo Genes (ambiguous residues) :: 0 of 36
            Pseudo Genes (frameshifted)       :: 2 of 36
            Pseudo Genes (incomplete)         :: 32 of 36
            Pseudo Genes (internal stop)      :: 2 of 36
            ##Genome-Annotation-Data-END##
FEATURES             Location/Qualifiers
     source          1..2825
                     /organism="Micropruina sp."
                     /mol_type="genomic DNA"
                     /submitter_seqid="NODE_14348_length_2825_cov_3.097473"
                     /isolate="AMR_MDS_2195"
                     /isolation_source="Activated sludge of municipal
                     wastewater treatment plants"
                     /db_xref="taxon:2737536"
                     /environmental_sample
                     /geo_loc_name="Canada"
                     /lat_lon="49.25 N 123.1 W"
                     /collection_date="2015-06-16"
                     /metagenome_source="wastewater metagenome"
                     /note="metagenomic"
     gene            complement(<1..541)
                     /locus_tag="PLF56_12110"
     CDS             complement(<1..541)
                     /locus_tag="PLF56_12110"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_014846536.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_function: GO:0003677 - DNA binding [Evidence IEA];
                     GO_process: GO:0000160 - phosphorelay signal transduction
                     system [Evidence IEA];
                     GO_process: GO:0006355 - regulation of DNA-templated
                     transcription [Evidence IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="response regulator transcription factor"
                     /protein_id="HOQ54342.1"
                     /translation="MLQLVLEADGFDTAWCSSGTRALDLFKRYQPDLVLLDLMLPGRD
                     GVDVCRDIRATSGVPIVMLTAKSDTRDVVEGLEAGADDYVAKPFKVRELLARIRTRLR
                     RHVPSDNSGQLAIGDLVINVPEHTVRRGTELIVLTPLEFDLLHALARRPKHAFTREML
                     LQEVWGYHHPSDTRLVNVHV"
     gene            complement(676..1236)
                     /locus_tag="PLF56_12115"
     CDS             complement(676..1236)
                     /locus_tag="PLF56_12115"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_013160872.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="YqgE/AlgH family protein"
                     /protein_id="HOQ54343.1"
                     /translation="MTEAATPGVLLVAGTELSDGVFDQTVVLVLDADESGSLGVVLNR
                     EATIDLRSALPAWAPLVSEPKVLFEGGPVSQEGAICLASPRRPEEEPPGWRSLFDAVG
                     LLHLDTPVEIAEGAYRDLRIFAGYAGWAPGQLEHELDEGWWHVVRATYADVYDTDPDT
                     LWRRVLRRQGGDLALLASWTPTPERN"
     gene            complement(1365..1734)
                     /gene="ssrA"
                     /locus_tag="PLF56_12120"
     tmRNA           complement(1365..1734)
                     /gene="ssrA"
                     /locus_tag="PLF56_12120"
                     /product="transfer-messenger RNA"
                     /inference="COORDINATES: nucleotide
                     motif:Rfam:14.4:RF00023"
                     /inference="COORDINATES: profile:INFERNAL:1.1.4"
                     /note="Derived by automated computational analysis using
                     gene prediction method: cmsearch."
                     /db_xref="RFAM:RF00023"
     gene            complement(1797..2417)
                     /locus_tag="PLF56_12125"
     CDS             complement(1797..2417)
                     /locus_tag="PLF56_12125"
                     /inference="COORDINATES: protein motif:HMM:NF021221.2"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="DUF1707 and DUF4870 domain-containing protein"
                     /protein_id="HOQ54344.1"
                     /translation="MTNDFFAPIDAVTKSDRDAAERRLREAHALGRIDAADFEARMAR
                     VITAERLDELAAATDGPLPAPAAQQYRSYREPEAATYAGTVMTAPRSSADQGTTMAVI
                     AHLSPFLTWLIGPAVIWAISAKDSYAKREAAKAFNWQLIAFVVGIVTGALGGLLPGDG
                     NPLARIWTVIWVVVTVIGAVKAAKGADWRNPIRALVPLEVLPERKR"
     gene            2522..>2825
                     /locus_tag="PLF56_12130"
     CDS             2522..>2825
                     /locus_tag="PLF56_12130"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_011753615.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_function: GO:0046872 - metal ion binding [Evidence
                     IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="maleylpyruvate isomerase N-terminal
                     domain-containing protein"
                     /protein_id="HOQ54345.1"
                     /translation="MPNSLAFADLGEGIGDATAVLRNNAASAGLDVPIPTCAGWTMRD
                     LVTHQGLVHRWAAATVRGERPRADPDVEAEAAAASDLLEWLDDGMVDLLNALATAPE"
BASE COUNT          438 a          962 c          972 g          453 t
ORIGIN      
        1 gcacatgcac attgaccagc cgggtgtcgg acgggtggtg gtagccccac acctcctgga
       61 gcagcatttc gcgggtgaag gcgtgcttgg gacggcgggc cagcgcgtgc agcaggtcga
      121 actcgagcgg ggtcaggacg atcagctcgg tgccgcggcg cacggtgtgt tcgggcacgt
      181 tgatcaccag gtcgccgatg gccaactggc cggagttgtc gctgggcaca tggcggcgca
      241 accgggtgcg gatgcgggcc agcagctcgc gcaccttgaa cggcttggcc acgtagtcgt
      301 ccgcgcctgc ctccaatccc tcgaccacgt cacgggtgtc ggacttggcg gtgagcatga
      361 cgatcggcac ccccgaggtg gcgcggatgt cgcggcacac gtccaccccg tcacgaccgg
      421 gcagcatcag gtcgagcagc accaggtcgg gctggtaccg cttgaacagg tccagggcgc
      481 gcgtgcccga actgcaccag gccgtgtcga aaccgtcagc ctccagcacc aactgcagca
      541 tctcggacag tgcgacgtcg tcgtcgacca cgaggatacg ttgcgtcgtc tccgacactg
      601 ccacctcact tgcgaaagcc tggggcccct gcgtgggctc ctcccctggg tgggagtgta
      661 gtcggcgggt cgtgatcagt tgcgttccgg ggtcggggtc caactggcca acaacgccag
      721 gtcgccgccc tgccggcgca acacccggcg ccagagcgtg tcggggtcgg tgtcgtacac
      781 gtcggcgtag gtggcccgca ccacgtgcca ccagccctcg tccagttcgt gttccagctg
      841 gcccggggcc cagcccgcgt agccggcgaa gatgcgcagg tcgcggtagg cgccctcggc
      901 gatctcgacc ggcgtgtcga ggtgcagcag gccgacggcg tcgaacagcg agcgccagcc
      961 gggcggctcc tcctccggac gacgcggcga ggcgagacag atcgcgccct cctgcgagac
     1021 cggcccgccc tcgaacagca ccttgggttc gctcaccagg ggcgcccagg cgggcagtgc
     1081 gctgcgcagg tcgatggtgg cctcacggtt gagcaccacg ccgagcgaac cggactcgtc
     1141 cgcgtccagc acgagcacga cggtctgatc gaacacgccg tcgctcagtt cggtgccggc
     1201 aaccagcaac accccggggg tggccgcctc ggtcactgcg ctcgcccctg catggcctca
     1261 agcatgccat tgccgaagcg ctgtgcgcag tgacccaaga gggtgcagcc cgggctcggg
     1321 acgcccgaag gccccgtccg atcggacggg gccttcgcgc tcacaggtgg aggtgggggg
     1381 agtcgaaccc ccgtcctcag aaggtgaacc aggacttctc cgggcgcagt tgacctgacg
     1441 gttttctcgg cttctaccct cgggtcaacc cgtggtagcc aagcccagtt ccagtgaagt
     1501 cccgagccgc ccctggaacc caggcggctc agcaagcctt ctaaacgagg ccagtgatcg
     1561 gacggaaggc tacgcccgga ctgacccttc ggtcactgct caggcagcga gagcgaagtc
     1621 tgcgcgagaa ttgtcggcag ttattggttt tccaaggatc gttaacgaga tgtccttgga
     1681 tcctcggccc gcttctcctg ggactaccgt cccgagtcga aaccagtcac cccctattga
     1741 gttgttgctg ttgcgttgtt gctgctcatc ggtcgcccgg tgagcagacc agactatcaa
     1801 cgtttgcgct cgggcaaaac ttccagcggc accagggcac ggatcgggtt gcgccagtcg
     1861 gcacccttag ccgccttgac cgcaccgatc accgtcacga ccacccagat caccgtccag
     1921 atccgggcca acgggttgcc gtcgccgggc agcagtcccc cgagcgcgcc tgtcacgatc
     1981 ccgaccacga aggcgatcag ctgccagttg aacgccttgg ccgcctcgcg cttggcatag
     2041 gaatccttcg cgctgatcgc ccagatcacc gcgggcccga tcagccaggt caggaacggc
     2101 gacaggtgag cgatcaccgc catcgtggtg ccctgatcgg cggacgagcg cggagccgtc
     2161 atgacggtgc cggcgtaggt cgccgcctcc ggttcgcggt aggaacggta ctgctgggcg
     2221 gccggtgcgg gcagcgggcc gtcggtggcg gcggccagtt cgtcgagccg ctcggcggtg
     2281 atcacccgcg ccatccgggc ctcgaaatcg gccgcgtcga tccggcccag ggcgtgtgcc
     2341 tcgcggagtc gccgttcggc ggcgtcgcgg tcgctcttgg tgacggcgtc gatcggtgcg
     2401 aagaagtcgt tggtcatggc tgccattgtg ctcgggccgt cgtcggcggc gtatcgggca
     2461 ctgccctgcc cgtgccccgg aaccgccctc ggggtagcgg cgtccggcat aggttgaagg
     2521 catgcccaac tcgctcgcat tcgccgatct cggcgaggga atcggtgatg ccaccgccgt
     2581 gcttcgcaac aacgccgcca gtgccggtct ggacgtgccg atccccacct gtgctggctg
     2641 gacgatgcgt gacctggtga cccatcaggg cctcgtgcac cggtgggcgg ccgcgacggt
     2701 acggggcgag cggccgcgcg cggatcccga tgtcgaggcc gaggccgcgg cggcgtccga
     2761 tctgctggag tggctggacg acggcatggt cgacctgctc aacgcgctcg cgaccgcgcc
     2821 cgaag
//
LOCUS       DAOSUG010000422         2823 bp    DNA     linear   ENV 10-JUN-2024
DEFINITION  MAG TPA_asm: Micropruina sp. isolate AMR_MDS_2195
            NODE_14359_length_2823_cov_3.946532, whole genome shotgun sequence.
ACCESSION   DAOSUG010000422 DAOSUG010000000
VERSION     DAOSUG010000422.1
DBLINK      BioProject: PRJNA925477
            BioSample: SAMN32864262
            Sequence Read Archive: SRR4236673
KEYWORDS    WGS; ENV; Metagenome Assembled Genome; MAG; Third Party Data; TPA;
            TPA:assembly.
SOURCE      Micropruina sp. (wastewater metagenome)
  ORGANISM  Micropruina sp.
            Bacteria; Bacillati; Actinomycetota; Actinomycetes;
            Propionibacteriales; Nocardioidaceae; Micropruina.
REFERENCE   1  (bases 1 to 2823)
  AUTHORS   Abdulkadir,N., Saraiva,J.P., Zhang,J., Stolte,S., Gillor,O.,
            Harms,H. and Rocha,U.
  TITLE     Genome-centric analyses of 165 metagenomes show that mobile genetic
            elements are crucial for the transmission of antimicrobial
            resistance genes to pathogens in activated sludge and wastewater
  JOURNAL   Microbiol Spectr 12 (3), e0291823 (2024)
   PUBMED   38289113
REFERENCE   2  (bases 1 to 2823)
  AUTHORS   Abdulkadir,N.
  TITLE     Direct Submission
  JOURNAL   Submitted (24-MAR-2023) Environmental Microbiology, Helmholtz
            Center for Environmental Research -UFZ, Permoserstr 15, Leipzig
            04318, Germany
COMMENT     The annotation was added by the NCBI Prokaryotic Genome Annotation
            Pipeline (PGAP). Information about PGAP can be found here:
            https://www.ncbi.nlm.nih.gov/genome/annotation_prok/
            
            ##Genome-Assembly-Data-START##
            Assembly Method        :: SPAdes v. 3.11.1
            Genome Representation  :: Full
            Expected Final Version :: Yes
            Genome Coverage        :: Not Applicable
            Sequencing Technology  :: Illumina MiSeq
            ##Genome-Assembly-Data-END##
            
            ##Genome-Annotation-Data-START##
            Annotation Provider               :: NCBI
            Annotation Date                   :: 05/04/2023 04:57:10
            Annotation Pipeline               :: NCBI Prokaryotic Genome
                                                 Annotation Pipeline (PGAP)
            Annotation Method                 :: Best-placed reference protein
                                                 set; GeneMarkS-2+
            Annotation Software revision      :: 6.5
            Features Annotated                :: Gene; CDS; rRNA; tRNA; ncRNA
            Genes (total)                     :: 2,776
            CDSs (total)                      :: 2,750
            Genes (coding)                    :: 2,714
            CDSs (with protein)               :: 2,714
            Genes (RNA)                       :: 26
            tRNAs                             :: 24
            ncRNAs                            :: 2
            Pseudo Genes (total)              :: 36
            CDSs (without protein)            :: 36
            Pseudo Genes (ambiguous residues) :: 0 of 36
            Pseudo Genes (frameshifted)       :: 2 of 36
            Pseudo Genes (incomplete)         :: 32 of 36
            Pseudo Genes (internal stop)      :: 2 of 36
            ##Genome-Annotation-Data-END##
FEATURES             Location/Qualifiers
     source          1..2823
                     /organism="Micropruina sp."
                     /mol_type="genomic DNA"
                     /submitter_seqid="NODE_14359_length_2823_cov_3.946532"
                     /isolate="AMR_MDS_2195"
                     /isolation_source="Activated sludge of municipal
                     wastewater treatment plants"
                     /db_xref="taxon:2737536"
                     /environmental_sample
                     /geo_loc_name="Canada"
                     /lat_lon="49.25 N 123.1 W"
                     /collection_date="2015-06-16"
                     /metagenome_source="wastewater metagenome"
                     /note="metagenomic"
     gene            complement(204..632)
                     /gene="ruvX"
                     /locus_tag="PLF56_12135"
     CDS             complement(204..632)
                     /gene="ruvX"
                     /locus_tag="PLF56_12135"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_014846654.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_component: GO:0005737 - cytoplasm [Evidence IEA];
                     GO_function: GO:0003674 - molecular_function [Evidence
                     IEA];
                     GO_process: GO:0008150 - biological_process [Evidence
                     IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="Holliday junction resolvase RuvX"
                     /protein_id="HOQ54346.1"
                     /translation="MALDWGKARIGVAACDPDGTLAYPVETVQAHTAARRLPQLLAEY
                     EPIEVIIGLPRTLAGDEGPSAGYVRGQASALIGAYPLLSWRFSDERLTTVAAGRRLQS
                     AGRDTRRQRAVIDQAAAVAILEQALAVERATGRAPGDPAQ"
     gene            complement(854..>2823)
                     /gene="alaS"
                     /locus_tag="PLF56_12140"
     CDS             complement(854..>2823)
                     /gene="alaS"
                     /locus_tag="PLF56_12140"
                     /EC_number="6.1.1.7"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_008599692.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_component: GO:0005737 - cytoplasm [Evidence IEA];
                     GO_function: GO:0004813 - alanine-tRNA ligase activity
                     [Evidence IEA];
                     GO_process: GO:0006419 - alanyl-tRNA aminoacylation
                     [Evidence IEA]"
                     /codon_start=3
                     /transl_table=11
                     /product="alanine--tRNA ligase"
                     /protein_id="HOQ54347.1"
                     /translation="GMGLERTAYLLQGVANMYEIDEIFPVITRAAELAGTSYGRDAQN
                     DVRLRVVGDHVRSALMLMTDGVTPGNEARGYVLRRLLRRSIRSMRLLGVHDPVLPELL
                     PVSKALMEVSYPEVTSQWERVSQTSYAEEQAFRRTLAAGTQIFDLAAGQAKREHAGVL
                     GGEQAFQLHDTYGFPIDLTLEMAAEAGLEVDQQGFRTLMQQQKDRAKADARAKKGGAV
                     NTDAYRTLRSTGETPFLGFTDLSVDTRVRGIVADGAAVTSARPGDVVEVVLDETPFYA
                     EAGGQDADSGVLTATGLELDVLDVQRPIQGLVVHKVLVNEGELSTGQRVLAQVDAPAR
                     FAASQAHTSTHIVNAGLRQLLGQGTHQAGSYNKPGYLRFDFNASQALSPSMRSELEGI
                     VNAAIRDDFEVTDRELPLDEAKALGAQAMFGEKYGNVVRMVELAGPWSRELCGGTHVV
                     RTSQIGLVSLLGEQSVGSGIRRVEALVSTEAFDRFAAERALVATLADTLKTQPDQLTD
                     RVEKLLAQLKAAEKQIENYRSKELLAGASGLATKASPAGRIRLVAEQLRGASGADLRT
                     VALQVRSELGSDAAVVALLGGADDKPVLVVATNEAARTAGARAGRLVSLGAAELGGRG
                     GGKDDFAQGGGTDGAGAARALDAIRTAVTAL"
BASE COUNT          403 a         1057 c          944 g          419 t
ORIGIN      
        1 aaaggcgatg taggcgtcgt gcacctttcc gtagacgaac cagccgccgc caagcagcac
       61 cgccagcgac agcaccaccg ccaccgaccc ccggacccgg taccagacct ctttgggcac
      121 ccatcggccg gtttcgggat cccgcaactc gcggaacaac ttcgccatca gttcgtcctt
      181 tgctggtcgc gccggttgtt tcgtcactgc gccgggtcgc cgggagccct gcccgtggca
      241 cgctccacgg ccaacgcctg ctccaagatt gccacggccg ccgcctggtc gatgacggcg
      301 cgctggcgac gggtgtcgcg accggccgac tgcaaccgtc ggccggcggc aacggtggtc
      361 agccgttcgt cgctgaaccg ccagctgagc agcggatacg caccgatcag cgccgaggcc
      421 tgcccccgca cataacctgc ggacggcccc tcgtcgccgg ccagggtgcg gggcaggccg
      481 atgatcacct cgatcggctc gtactcggcc agcagttgcg gcaaccgccg ggccgcggtg
      541 tgggcctgga cggtctcgac cgggtacgcg agcgtgccgt cggggtcgca ggcggccacc
      601 ccgatccgcg ccttgcccca gtcgagcgcc aaccggacgc cgcgccgaaa ggtgggccgg
      661 tgatcgagcg tgccgagatc cgtccgatga tcgagcgagt cgagctccgt ccgatgatcg
      721 agcgtgtcga gatcctccgc atgcgcagcc agggtctcga cgggctcgac cggctcaacc
      781 cgcgaaacgg tctcgacagg ctccacccgc gaaccagtct cgaccggcga gcgctgggcc
      841 tcggcgtcgc cggtcaaaga gcagtcaccg cggtccggat ggcgtccaac gcacgcgccg
      901 cgccggcgcc gtcggtgccg ccaccctgcg cgaagtcgtc cttgccgccg ccacgtccac
      961 caagttcggc cgcaccgagc gagaccagtc gccccgcgcg cgcgcccgcc gtgcgggcgg
     1021 cctcattggt ggccacgacc agcaccggct tgtcgtcggc gccaccgagc agcgcgacca
     1081 cggcggcgtc cgagcccagt tcgctgcgca cctgcaaggc gactgtgcgc aggtcggcac
     1141 cggaggcacc gcgcaactgc tcggccacca gccggatgcg gccggccggc gacgccttgg
     1201 tggccagccc ggacgccccg gccagcagtt ccttgctgcg gtagttctcg atctgcttct
     1261 cggccgcctt caactgggcc aagagcttct cgacccggtc ggtcaactgg tccggctgcg
     1321 tcttcagggt gtcggcgagc gtcgcgacca gcgcacgctc ggcggcgaac cggtcgaacg
     1381 cctcggtgct gaccagcgcc tcgacccggc ggatgcccga gccgaccgac tgctcaccca
     1441 gcaggctgac cagcccgatc tgcgaggtgc gcaccacgtg ggtgccgccg cacagttcgc
     1501 gcgaccacgg ccccgccagt tcgaccatgc gcaccacgtt gccgtacttc tcgccgaaca
     1561 tcgcctgcgc cccgagcgcc ttggcctcat cgagcggcag ctctcgatcg gtcacctcga
     1621 agtcgtcgcg gatcgcggcg ttgacgatgc cctccagctc gctgcgcatg ctgggcgaga
     1681 gcgcctgcga cgcgttgaag tcgaagcgca ggtagcccgg cttgttgtac gagccggcct
     1741 ggtgagtgcc ttgccccagc aactgccgca ggccggcgtt cacgatatgc gtggacgtgt
     1801 gggcctggct ggccgcgaac cgggcggggg cgtccacctg ggccagcacg cgctgtccgg
     1861 tgctcagctc accctcgttc accagcacct tgtgcaccac caggccctgg atcggacgtt
     1921 gcacgtcgag cacgtccaac tcgaggccgg tggccgtgag cacgccggag tcggcgtcct
     1981 ggccgcccgc ctcggcgtag aacggtgtct cgtcgagcac cacctcgacc acgtcgcccg
     2041 gacgcgcgga ggtcaccgcg gcgccgtccg ccacgatgcc ccgcacccgg gtgtcgacgc
     2101 tcaggtcggt gaaccccagg aacggcgtct caccggtgct gcgcagcgtc cggtaggcgt
     2161 cggtgttcac cgcgccgccc ttcttggccc tcgcgtcggc cttggcgcga tccttctgct
     2221 gctgcatcag cgtgcggaag ccctgctgat cgacctcgag gccggcctcg gcggccatct
     2281 cgagggtcag gtcgatggga aagccgtagg tgtcgtgcaa ctggaacgcc tgctcgccgc
     2341 cgagcacacc ggcgtgctcg cgcttggcct gaccggccgc caggtcgaag atctgcgtgc
     2401 cggccgccag ggtgcggcgg aacgcctgct cttcggcgta actggtctgc gacacccgct
     2461 cccactggct ggtcacctcg gggtagctga cctccatcag cgccttcgac accggcagca
     2521 gctccggcaa caccgggtcg tgcacgccga gcagccgcat cgagcggatc gagcgccgca
     2581 gcagccgacg cagcacgtag ccgcgcgcct cgttgccggg cgtcacgccg tcggtcatca
     2641 gcatcagcgc cgagcggacg tggtcgccga ccacccgcaa ccgcacgtcg ttctgggcgt
     2701 cgcggccgta gctggtgccg gcgagttcgg cggcccgggt gatgaccgga aagatctcgt
     2761 cgatctcgta catgttggcc acgccctgca gcaggtaggc ggtgcgttcc aggcccatgc
     2821 cgg
//
LOCUS       DAOSUG010000423         2814 bp    DNA     linear   ENV 10-JUN-2024
DEFINITION  MAG TPA_asm: Micropruina sp. isolate AMR_MDS_2195
            NODE_14409_length_2814_cov_4.229431, whole genome shotgun sequence.
ACCESSION   DAOSUG010000423 DAOSUG010000000
VERSION     DAOSUG010000423.1
DBLINK      BioProject: PRJNA925477
            BioSample: SAMN32864262
            Sequence Read Archive: SRR4236673
KEYWORDS    WGS; ENV; Metagenome Assembled Genome; MAG; Third Party Data; TPA;
            TPA:assembly.
SOURCE      Micropruina sp. (wastewater metagenome)
  ORGANISM  Micropruina sp.
            Bacteria; Bacillati; Actinomycetota; Actinomycetes;
            Propionibacteriales; Nocardioidaceae; Micropruina.
REFERENCE   1  (bases 1 to 2814)
  AUTHORS   Abdulkadir,N., Saraiva,J.P., Zhang,J., Stolte,S., Gillor,O.,
            Harms,H. and Rocha,U.
  TITLE     Genome-centric analyses of 165 metagenomes show that mobile genetic
            elements are crucial for the transmission of antimicrobial
            resistance genes to pathogens in activated sludge and wastewater
  JOURNAL   Microbiol Spectr 12 (3), e0291823 (2024)
   PUBMED   38289113
REFERENCE   2  (bases 1 to 2814)
  AUTHORS   Abdulkadir,N.
  TITLE     Direct Submission
  JOURNAL   Submitted (24-MAR-2023) Environmental Microbiology, Helmholtz
            Center for Environmental Research -UFZ, Permoserstr 15, Leipzig
            04318, Germany
COMMENT     The annotation was added by the NCBI Prokaryotic Genome Annotation
            Pipeline (PGAP). Information about PGAP can be found here:
            https://www.ncbi.nlm.nih.gov/genome/annotation_prok/
            
            ##Genome-Assembly-Data-START##
            Assembly Method        :: SPAdes v. 3.11.1
            Genome Representation  :: Full
            Expected Final Version :: Yes
            Genome Coverage        :: Not Applicable
            Sequencing Technology  :: Illumina MiSeq
            ##Genome-Assembly-Data-END##
            
            ##Genome-Annotation-Data-START##
            Annotation Provider               :: NCBI
            Annotation Date                   :: 05/04/2023 04:57:10
            Annotation Pipeline               :: NCBI Prokaryotic Genome
                                                 Annotation Pipeline (PGAP)
            Annotation Method                 :: Best-placed reference protein
                                                 set; GeneMarkS-2+
            Annotation Software revision      :: 6.5
            Features Annotated                :: Gene; CDS; rRNA; tRNA; ncRNA
            Genes (total)                     :: 2,776
            CDSs (total)                      :: 2,750
            Genes (coding)                    :: 2,714
            CDSs (with protein)               :: 2,714
            Genes (RNA)                       :: 26
            tRNAs                             :: 24
            ncRNAs                            :: 2
            Pseudo Genes (total)              :: 36
            CDSs (without protein)            :: 36
            Pseudo Genes (ambiguous residues) :: 0 of 36
            Pseudo Genes (frameshifted)       :: 2 of 36
            Pseudo Genes (incomplete)         :: 32 of 36
            Pseudo Genes (internal stop)      :: 2 of 36
            ##Genome-Annotation-Data-END##
FEATURES             Location/Qualifiers
     source          1..2814
                     /organism="Micropruina sp."
                     /mol_type="genomic DNA"
                     /submitter_seqid="NODE_14409_length_2814_cov_4.229431"
                     /isolate="AMR_MDS_2195"
                     /isolation_source="Activated sludge of municipal
                     wastewater treatment plants"
                     /db_xref="taxon:2737536"
                     /environmental_sample
                     /geo_loc_name="Canada"
                     /lat_lon="49.25 N 123.1 W"
                     /collection_date="2015-06-16"
                     /metagenome_source="wastewater metagenome"
                     /note="metagenomic"
     gene            complement(35..277)
                     /locus_tag="PLF56_12145"
     CDS             complement(35..277)
                     /locus_tag="PLF56_12145"
                     /inference="COORDINATES: ab initio
                     prediction:GeneMarkS-2+"
                     /note="Derived by automated computational analysis using
                     gene prediction method: GeneMarkS-2+.
                     GO_function: GO:0003677 - DNA binding [Evidence IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="helix-turn-helix domain-containing protein"
                     /protein_id="HOQ54348.1"
                     /translation="MTALTMTRSRKTTQQSTFGRTSKWLSYPEAAELSTLSVRSLKRL
                     TAAGKLPCYVVGGKVLRLKASDVEALFVPADECRGR"
     gene            415..1689
                     /gene="istA"
                     /locus_tag="PLF56_12150"
     CDS             415..1689
                     /gene="istA"
                     /locus_tag="PLF56_12150"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_103557054.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_function: GO:0004803 - transposase activity [Evidence
                     IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="IS21 family transposase"
                     /protein_id="HOQ54349.1"
                     /translation="MITLENWAEIRRLYKSEGLSQSAIARELGIARNTVASALKTDTP
                     PRYVRPPKGSLVDAVEPQIRLLLKEHPKMPATVIAERVGWQHSMTILKERVRELRPLF
                     VGVDPTDRVVYEPGQVAQCDLWFPPLLIPVGGGQAQILPVLAMTCGYSRITGGAMIPS
                     RQGGDILAGMWAILGAWGASPRTLVWDREAAIGGTGKLTVPAATFAGTLGVRIKLAPP
                     RDPEFKGLVERRNGFFETSFLPGRTFTGPGDFNDQFTDWIDQKANRRHLRAIGATPRE
                     RWDDDKSAMVALPPFSPPVGLTNRIRLGRDYYVRIGSNDYSVDPSAVGRFVEVVATPD
                     VVAVTCEDRIVATHARHWGSGATITDPDHVATAKELRGSFNAARAAAAARKTSRAHGD
                     GHVVALRALPDYDDLFGVDFHHAPDLQGVHTS"
     gene            1686..2462
                     /gene="istB"
                     /locus_tag="PLF56_12155"
     CDS             1686..2462
                     /gene="istB"
                     /locus_tag="PLF56_12155"
                     /inference="COORDINATES: protein motif:HMM:NF005098.0"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_function: GO:0005524 - ATP binding [Evidence IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="IS21-like element helper ATPase IstB"
                     /protein_id="HOQ54350.1"
                     /translation="MTAIATDVSSKIAYLARALKTPTIARTWEDIAVQARDLGWSHEE
                     YLAAVLERQNSEREASGAMMRIKTAHFPAIKTLEDFNYDHLPSLRRDILAHLATATYV
                     AKAENVILLGPPGIGKTHLAIGLGVKAAQNGHSVLFDTASNWITRLAGAHQAGTLDAE
                     LKKIRRYKLIIIDEVGYIPFDTDAANLFFQLIATRYEQGSIIVTSNLPFGRWGEAFSD
                     DVVAAAMIDRLVHHAEVIALSGESYRTRARRELLAKNGNK"
     gene            complement(2507..2752)
                     /locus_tag="PLF56_12160"
     CDS             complement(2507..2752)
                     /locus_tag="PLF56_12160"
                     /inference="COORDINATES: protein motif:HMM:NF018749.2"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="DUF1349 domain-containing protein"
                     /protein_id="HOQ54351.1"
                     /translation="METVDWATGRWTSPPVRSQQTPGGLLVEAKEGSDAWRTTSYGFI
                     HDDEHALIAPLGREQAIEVDFWARFSQQFDPAGVNGG"
BASE COUNT          496 a          978 c          892 g          448 t
ORIGIN      
        1 cgcgcactcc aggaatccag atgcggttag agggtcaccg tcctcgacac tcgtccgcgg
       61 gcacgaacag ggcctccacg tcggacgcct tgagccgcag caccttgcct cccaccacgt
      121 agcacggcag cttccccgcc gcggtcagcc gtttcagcga ccgaaccgac aacgtggaga
      181 gttccgccgc ctcagggtag gacaaccact tcgacgtgcg accgaacgtc gactgctggg
      241 tggtcttcct cgaacgagtc atcgtcaggg cggtcatgtc atctcctcgg tgatcgacgg
      301 acgctggtgt cgtccttcac cctccagaag tgaccaagga ggctgtcgac gacgcgtgaa
      361 aactgggcag taatcgacgg gtgaaagttg agcaccccga gtacgccttg gggtgtgatc
      421 acgttggaga actgggccga gattcgtcgg ctgtacaagt ccgaggggct ctcgcagtcc
      481 gctattgcac gcgagttggg gatcgcgagg aacacggtcg cgtcggcgct gaagaccgac
      541 acgccaccgc ggtacgtgcg gccgccgaag gggtcgctgg tcgacgccgt cgagccgcag
      601 atccggttgc tgctgaagga gcatccgaag atgccggcca cggtgatcgc cgagcgcgtc
      661 ggctggcagc actcgatgac gatcctcaag gaacgagtcc gtgagctgcg gcccttgttc
      721 gtcggtgtcg acccgaccga tcgggtcgtg tacgagcccg gtcaggtcgc tcaatgcgac
      781 ctgtggttcc cgcccctgct gatccctgtc ggcggcggtc aggcacagat cctgccggtg
      841 ttggcgatga cgtgcggcta ctcgcggatc acgggtggcg cgatgatccc gtcgcgtcag
      901 ggcggcgaca ttctggccgg catgtgggcc atcctcggcg cctggggcgc gagcccgcgg
      961 acgctggtgt gggaccgtga agcggcgatc ggtggcaccg gcaagctcac tgtgcctgct
     1021 gcgaccttcg ccggcaccct gggggtgcgg atcaagctcg ccccgccacg tgacccggag
     1081 ttcaagggcc tggtcgagcg ccgcaacggg ttcttcgaga cctcgttcct acccgggcgc
     1141 accttcactg gccccggcga cttcaacgac cagttcactg actggatcga ccagaaggcc
     1201 aaccggcgcc acctgcgcgc gatcggcgcc accccgcgtg agcggtggga cgacgacaag
     1261 tctgcgatgg tcgccctgcc accgttcagt ccgccggtgg ggctgacgaa caggatccgc
     1321 ctgggtcggg actactacgt gcggatcggc tcgaacgact actccgtgga cccgtccgcg
     1381 gtcggcaggt tcgtcgaggt ggtcgcgact cctgacgtcg tcgcggtcac ctgcgaggac
     1441 cggatcgtcg cgacccatgc ccggcattgg ggaagcggcg ccacgatcac cgacccggac
     1501 cacgtcgcga cggcgaagga gctgcggggc tcgttcaatg cggcgcgtgc tgctgcggcg
     1561 gcacgtaaga ccagccgcgc ccacggcgac gggcacgtcg tcgcgctacg cgccctgccc
     1621 gactacgacg acctcttcgg cgtcgacttc caccacgccc ccgacctgca aggagtccac
     1681 acctcatgac cgctatcgcc accgatgtgt cgtcgaagat cgcctacctg gcccgcgcac
     1741 tgaagacccc caccattgcc cgcacgtggg aagacatcgc ggtccaagcg cgcgacctgg
     1801 gctggtcgca tgaggagtac ctcgccgcgg tcctggaacg ccagaacagc gaacgcgagg
     1861 catcgggggc gatgatgcgg atcaagaccg ctcacttccc ggccatcaag accctcgagg
     1921 acttcaacta cgaccacctg ccgtcgctgc gccgcgacat tctcgcgcac ctagccaccg
     1981 cgacctacgt cgcgaaggcc gagaacgtga tcctgctcgg cccgcccggg atcggcaaga
     2041 cacacctcgc gatcgggctg ggcgtcaagg ccgcacagaa cggccactcc gtgctgttcg
     2101 acaccgcgag caactggatc acccggctgg ccggcgccca ccaggccgga acgctggacg
     2161 ccgagctcaa aaagatccgc cgctacaagc tcatcatcat cgacgaggtc ggctacatcc
     2221 ccttcgacac cgacgccgcg aacctgttct tccaactcat cgcgacccgc tacgagcaag
     2281 gctcgatcat cgtcacatcg aacctgccct tcggccgctg gggcgaggcc ttcagcgacg
     2341 acgtcgtggc cgccgcgatg atcgaccgac tcgtccacca cgccgaggtc attgccctgt
     2401 caggagagtc ctacagaacc cgcgcccgcc gcgaactcct cgccaagaac ggcaacaagt
     2461 agacgaccag gtgctcaaat ttcacccgtc gaaacctgct caattttcag ccgccgttga
     2521 cacccgcggg gtcgaactgc tggctgaacc gggcccagaa gtccacctcg atcgcctgct
     2581 cgcggcccaa cggtgcgatc agggcgtgtt cgtcgtcgtg aatgaagcca taggaggtcg
     2641 tccgccaggc gtccgagccc tcctttgcct cgacaagcag cccaccgggg gtctgctgac
     2701 tgcgcacggg tgggctggtc cagcggcccg tggcccagtc gacggtctcc acgcgccgac
     2761 cctaatggtc tgggcgaacc ggggtcggtt tgcaccgcag tcggttcaaa ccaa
//
LOCUS       DAOSUG010000424         2794 bp    DNA     linear   ENV 10-JUN-2024
DEFINITION  MAG TPA_asm: Micropruina sp. isolate AMR_MDS_2195
            NODE_14561_length_2794_cov_4.767798, whole genome shotgun sequence.
ACCESSION   DAOSUG010000424 DAOSUG010000000
VERSION     DAOSUG010000424.1
DBLINK      BioProject: PRJNA925477
            BioSample: SAMN32864262
            Sequence Read Archive: SRR4236673
KEYWORDS    WGS; ENV; Metagenome Assembled Genome; MAG; Third Party Data; TPA;
            TPA:assembly.
SOURCE      Micropruina sp. (wastewater metagenome)
  ORGANISM  Micropruina sp.
            Bacteria; Bacillati; Actinomycetota; Actinomycetes;
            Propionibacteriales; Nocardioidaceae; Micropruina.
REFERENCE   1  (bases 1 to 2794)
  AUTHORS   Abdulkadir,N., Saraiva,J.P., Zhang,J., Stolte,S., Gillor,O.,
            Harms,H. and Rocha,U.
  TITLE     Genome-centric analyses of 165 metagenomes show that mobile genetic
            elements are crucial for the transmission of antimicrobial
            resistance genes to pathogens in activated sludge and wastewater
  JOURNAL   Microbiol Spectr 12 (3), e0291823 (2024)
   PUBMED   38289113
REFERENCE   2  (bases 1 to 2794)
  AUTHORS   Abdulkadir,N.
  TITLE     Direct Submission
  JOURNAL   Submitted (24-MAR-2023) Environmental Microbiology, Helmholtz
            Center for Environmental Research -UFZ, Permoserstr 15, Leipzig
            04318, Germany
COMMENT     The annotation was added by the NCBI Prokaryotic Genome Annotation
            Pipeline (PGAP). Information about PGAP can be found here:
            https://www.ncbi.nlm.nih.gov/genome/annotation_prok/
            
            ##Genome-Assembly-Data-START##
            Assembly Method        :: SPAdes v. 3.11.1
            Genome Representation  :: Full
            Expected Final Version :: Yes
            Genome Coverage        :: Not Applicable
            Sequencing Technology  :: Illumina MiSeq
            ##Genome-Assembly-Data-END##
            
            ##Genome-Annotation-Data-START##
            Annotation Provider               :: NCBI
            Annotation Date                   :: 05/04/2023 04:57:10
            Annotation Pipeline               :: NCBI Prokaryotic Genome
                                                 Annotation Pipeline (PGAP)
            Annotation Method                 :: Best-placed reference protein
                                                 set; GeneMarkS-2+
            Annotation Software revision      :: 6.5
            Features Annotated                :: Gene; CDS; rRNA; tRNA; ncRNA
            Genes (total)                     :: 2,776
            CDSs (total)                      :: 2,750
            Genes (coding)                    :: 2,714
            CDSs (with protein)               :: 2,714
            Genes (RNA)                       :: 26
            tRNAs                             :: 24
            ncRNAs                            :: 2
            Pseudo Genes (total)              :: 36
            CDSs (without protein)            :: 36
            Pseudo Genes (ambiguous residues) :: 0 of 36
            Pseudo Genes (frameshifted)       :: 2 of 36
            Pseudo Genes (incomplete)         :: 32 of 36
            Pseudo Genes (internal stop)      :: 2 of 36
            ##Genome-Annotation-Data-END##
FEATURES             Location/Qualifiers
     source          1..2794
                     /organism="Micropruina sp."
                     /mol_type="genomic DNA"
                     /submitter_seqid="NODE_14561_length_2794_cov_4.767798"
                     /isolate="AMR_MDS_2195"
                     /isolation_source="Activated sludge of municipal
                     wastewater treatment plants"
                     /db_xref="taxon:2737536"
                     /environmental_sample
                     /geo_loc_name="Canada"
                     /lat_lon="49.25 N 123.1 W"
                     /collection_date="2015-06-16"
                     /metagenome_source="wastewater metagenome"
                     /note="metagenomic"
     gene            complement(363..995)
                     /locus_tag="PLF56_12165"
     CDS             complement(363..995)
                     /locus_tag="PLF56_12165"
                     /inference="COORDINATES: ab initio
                     prediction:GeneMarkS-2+"
                     /note="Derived by automated computational analysis using
                     gene prediction method: GeneMarkS-2+."
                     /codon_start=1
                     /transl_table=11
                     /product="transcriptional regulator"
                     /protein_id="HOQ54352.1"
                     /translation="MGATSDPALLALHGVRVLGGPSTAAVADRFGRPVGEVAEHLLDA
                     QAYGWVTRYDYFGETWALTERGRAENERQLAGELDGLGARPALIEAHRRFVALNGPHG
                     VACTNWQLRPLPGAPSAVNDHSDPVWDMRVLVELEVIDQSLTELVGSLTTVLNRFEGY
                     DRLHRQALGRVRRGAHEWLDSPDRNCCQLVWIQLHEDLLATLQIPRGSDG"
     gene            complement(998..1954)
                     /locus_tag="PLF56_12170"
     CDS             complement(998..1954)
                     /locus_tag="PLF56_12170"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_007077559.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="aldo/keto reductase"
                     /protein_id="HOQ54353.1"
                     /translation="MRFNPLGDSGLMVSAVGVGCNAFGARIDADQTKAVVDAAIEQGI
                     TLFDTADSYADGASEQLLGRALGKRREDVVVATKFGMGDHDAEHFGAHGSRRYVRRAV
                     EASLRRLGTDYIDLYQLHRPDPITPIDETLSAMSDLVAEGKVRYIGSSNLTAWQVADA
                     DWVARTSGLHRFVSAQNEYSLYNRAAEQELVPACAHFGIGLLPYFPLAYGLLTGKYRR
                     GEAAPAGSRLESQANRLQGADWDRIEALQAYADARGLSLLTVAIGGLAAQPAVASVIS
                     GVTRPEQIAANVEAGAWQPSAQDLAELDELRHTPAFSYTTFA"
     gene            2037..>2794
                     /locus_tag="PLF56_12175"
     CDS             2037..>2794
                     /locus_tag="PLF56_12175"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_017932938.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_function: GO:0003824 - catalytic activity [Evidence
                     IEA];
                     GO_process: GO:0005992 - trehalose biosynthetic process
                     [Evidence IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="trehalose-6-phosphate synthase"
                     /protein_id="HOQ54354.1"
                     /translation="MTEASFVVVANRLPVDRDADGGWKRSPGGLVTALEPVMRKADGA
                     WVGWPGKPDLDLEPLEVDGTDLIPVRLSAEEVELYYEGFSNDTIWPLYHDVIAAPSYR
                     RAWWDAYVRVNRRFAEAAARAAAEGGTVWVQDYQLQLVPAMLREERPDLVIGYFHHIP
                     FPAYGLFSQLPWRRQVAEGLLGADVIGFQRKADASNFAQAVRRLFRYETKAHGIRVPT
                     PDGGTRRALAKAFPISIDVESYEQLAASDEVKARA"
BASE COUNT          423 a          986 c          983 g          402 t
ORIGIN      
        1 ggactcagcg aaccaagccc ggacccaccg atgacccagc cggcgtgacc ggcgccggcc
       61 ggggctgggg agccggtcgt tgcggggccg tcacggtcgt ctgtcccaca gtggagacgc
      121 ggccggggcg gggatggccg cgcagcaggc ggacgttcgg tggagccggg cttcggagtc
      181 actcacccac ccacccgaac cgtctggcac gacgggactc ccgcctgaaa agcctgcacc
      241 agtggcagac ctcgcggcgc gcgcaggcat gaccccctgc actggtgcag ggatttcagg
      301 cggtgaccgt ggcgaccgtg cagtacccct cgctgatcgg cccgtcgctg atcggtgcgc
      361 cgctacccgt cgctgccgcg cggaatctgc agggtggcca gcaggtcctc gtgcagttgg
      421 atccagacca gttgacagca gttccgatcg ggggaatcga gccactcgtg ggcgccgcgc
      481 cgcacccggc ccagggcctg ccggtgcaac cggtcgtagc cctcgaaccg gttcagcacc
      541 gtggtgagtg agccgaccag ctcggtcagc gactggtcga tgacctcgag ttcgacgagc
      601 acccgcatgt cccagaccgg gtcggaatgg tcgttgaccg cgctcggcgc gccgggcagg
      661 gggcgcagct gccagttggt gcaggccacc ccgtgcgggc cgttcagcgc cacgaatcgg
      721 cggtgcgcct cgatcagcgc cgggcgcgcg ccgagcccgt ccagttcgcc ggcgagctgc
      781 cgctcgttct cggccctacc gcgctcggtc agcgcccagg tttcaccgaa gtagtcgtag
      841 cgggtcaccc agccgtaggc ctgcgcgtcc agcaggtgct cggcgacctc gccaacgggc
      901 cggccgaacc ggtcggcgac cgccgcggtg gacggtcccc cgagcacccg gacgccgtgc
      961 agggcaagca gtgcgggatc ggacgtggcg cccacgctca ggcgaacgtg gtgtacgaga
     1021 aggcgggcgt gtgacgcagt tcgtccagct cggccagatc ctgcgcggac ggctgccagg
     1081 ccccggcctc gacgttggcc gcgatctgct ccgggcgggt cacgccgctg atcacgctcg
     1141 ccaccgcggg ctgagcggcc aggccgccga tcgcgacggt cagcagcgac agcccgcgcg
     1201 cgtcggcgta ggcctgcagc gcctcgatcc gatcccagtc ggcaccctgc aaccggttgg
     1261 cctgcgattc caaccgcgac cccgccggcg ccgcctcacc gcgccggtac ttgccggtga
     1321 gcagcccgta ggccagcgga aagtagggca gcaggccgat gccgaagtgt gcgcaggcgg
     1381 gcaccagttc ctgctcggcg gcccggttgt acagcgaata ctcgttctgc gccgagacga
     1441 agcggtgcag accggacgtc cgtgccaccc agtcggcgtc cgccacctgc caggcggtca
     1501 ggttcgagga gccgatgtaa cgcaccttgc cctcggcgac caggtcgctc atcgcgctga
     1561 gggtctcgtc gatcggcgtg atcgggtcgg gacggtgcag ctggtacagg tcgatgtaat
     1621 cagtaccgag ccggcgcagg ctggcctcga ccgcgcgacg cacgtaccgt cgcgagccat
     1681 gggcaccgaa gtgctcggcg tcgtggtcgc ccatgccgaa cttggtggcc acgaccacgt
     1741 cctcgcgtcg cttgcccagg gcacggccga gcagttgctc gctggccccg tccgcatagg
     1801 agtcggcggt gtcgaacagg gtgatgccct gctcgatggc cgcatcgacc acggctttcg
     1861 tctggtcggc gtcgatcctg gcgccgaagg cgttgcagcc gacgccgacc gccgacacca
     1921 tcaggccgct gtctccaaga gggttgaacc gcatggggcg aacctaccct ctgcgcgcga
     1981 cgggcacgat ccggttagcc tgggcagcca cgaccccaac gccaggagac cccatcgtga
     2041 ccgaagccag tttcgtcgtc gtcgccaacc gattgccggt cgaccgggac gccgacggcg
     2101 gctggaagcg ctcccccggc ggcctggtca cggccctgga accggtgatg cgcaaggcgg
     2161 acggcgcctg ggtgggttgg ccgggcaagc ccgacctcga cctcgaaccg ctcgaggtcg
     2221 acggcaccga cctgattccg gtgcggctga gcgccgaaga ggtggagctg tactacgagg
     2281 gcttctccaa cgacaccatc tggccgctgt accacgacgt gatcgcggca cccagctacc
     2341 ggcgcgcctg gtgggacgcc tatgtgcggg tcaatcggcg cttcgccgag gccgccgcgc
     2401 gcgccgcagc cgagggcggc acggtctggg tgcaggacta ccagttgcag ttggtgccgg
     2461 cgatgctgcg cgaggagcgt ccggatctgg tgatcgggta cttccaccac attccgttcc
     2521 ccgcgtacgg actcttcagc cagttgccct ggcgccggca ggtagccgaa gggctgctcg
     2581 gtgccgacgt gatcggcttc caacgcaagg ccgatgccag caacttcgcc caggccgtcc
     2641 gccggttgtt ccgctacgag acgaaggcgc acggcatcag ggtgccgacc cccgacggcg
     2701 gcacgcggcg tgcgctggcc aaagccttcc cgatctcgat cgacgtcgag tcctacgaac
     2761 aactggccgc cagcgacgag gtgaaggcgc gggc
//
LOCUS       DAOSUG010000425         2791 bp    DNA     linear   ENV 10-JUN-2024
DEFINITION  MAG TPA_asm: Micropruina sp. isolate AMR_MDS_2195
            NODE_14580_length_2791_cov_4.088450, whole genome shotgun sequence.
ACCESSION   DAOSUG010000425 DAOSUG010000000
VERSION     DAOSUG010000425.1
DBLINK      BioProject: PRJNA925477
            BioSample: SAMN32864262
            Sequence Read Archive: SRR4236673
KEYWORDS    WGS; ENV; Metagenome Assembled Genome; MAG; Third Party Data; TPA;
            TPA:assembly.
SOURCE      Micropruina sp. (wastewater metagenome)
  ORGANISM  Micropruina sp.
            Bacteria; Bacillati; Actinomycetota; Actinomycetes;
            Propionibacteriales; Nocardioidaceae; Micropruina.
REFERENCE   1  (bases 1 to 2791)
  AUTHORS   Abdulkadir,N., Saraiva,J.P., Zhang,J., Stolte,S., Gillor,O.,
            Harms,H. and Rocha,U.
  TITLE     Genome-centric analyses of 165 metagenomes show that mobile genetic
            elements are crucial for the transmission of antimicrobial
            resistance genes to pathogens in activated sludge and wastewater
  JOURNAL   Microbiol Spectr 12 (3), e0291823 (2024)
   PUBMED   38289113
REFERENCE   2  (bases 1 to 2791)
  AUTHORS   Abdulkadir,N.
  TITLE     Direct Submission
  JOURNAL   Submitted (24-MAR-2023) Environmental Microbiology, Helmholtz
            Center for Environmental Research -UFZ, Permoserstr 15, Leipzig
            04318, Germany
COMMENT     The annotation was added by the NCBI Prokaryotic Genome Annotation
            Pipeline (PGAP). Information about PGAP can be found here:
            https://www.ncbi.nlm.nih.gov/genome/annotation_prok/
            
            ##Genome-Assembly-Data-START##
            Assembly Method        :: SPAdes v. 3.11.1
            Genome Representation  :: Full
            Expected Final Version :: Yes
            Genome Coverage        :: Not Applicable
            Sequencing Technology  :: Illumina MiSeq
            ##Genome-Assembly-Data-END##
            
            ##Genome-Annotation-Data-START##
            Annotation Provider               :: NCBI
            Annotation Date                   :: 05/04/2023 04:57:10
            Annotation Pipeline               :: NCBI Prokaryotic Genome
                                                 Annotation Pipeline (PGAP)
            Annotation Method                 :: Best-placed reference protein
                                                 set; GeneMarkS-2+
            Annotation Software revision      :: 6.5
            Features Annotated                :: Gene; CDS; rRNA; tRNA; ncRNA
            Genes (total)                     :: 2,776
            CDSs (total)                      :: 2,750
            Genes (coding)                    :: 2,714
            CDSs (with protein)               :: 2,714
            Genes (RNA)                       :: 26
            tRNAs                             :: 24
            ncRNAs                            :: 2
            Pseudo Genes (total)              :: 36
            CDSs (without protein)            :: 36
            Pseudo Genes (ambiguous residues) :: 0 of 36
            Pseudo Genes (frameshifted)       :: 2 of 36
            Pseudo Genes (incomplete)         :: 32 of 36
            Pseudo Genes (internal stop)      :: 2 of 36
            ##Genome-Annotation-Data-END##
FEATURES             Location/Qualifiers
     source          1..2791
                     /organism="Micropruina sp."
                     /mol_type="genomic DNA"
                     /submitter_seqid="NODE_14580_length_2791_cov_4.088450"
                     /isolate="AMR_MDS_2195"
                     /isolation_source="Activated sludge of municipal
                     wastewater treatment plants"
                     /db_xref="taxon:2737536"
                     /environmental_sample
                     /geo_loc_name="Canada"
                     /lat_lon="49.25 N 123.1 W"
                     /collection_date="2015-06-16"
                     /metagenome_source="wastewater metagenome"
                     /note="metagenomic"
     gene            232..1272
                     /gene="mgrA"
                     /locus_tag="PLF56_12180"
     CDS             232..1272
                     /gene="mgrA"
                     /locus_tag="PLF56_12180"
                     /EC_number="1.1.1.-"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_017934212.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_function: GO:0047834 - D-threo-aldose 1-dehydrogenase
                     activity [Evidence IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="L-glyceraldehyde 3-phosphate reductase"
                     /protein_id="HOQ54355.1"
                     /translation="MPLEPVYVPVPDRYDGRMPYRRTGRSGLLLPAISLGLWHNFGDD
                     VPFATQRATLRRAFDLGITHFDLANNYGPPYGSAETNFGRHLRDDFAPYRDQLVISSK
                     AGWDFWPGPYGDLGSRKYLIGSLDASLERMGLDYVDIFYHHRFDPDTPLEETMGALDY
                     AVRSGRARYIGISSYSGTRTREAVAILRELGTPLLIHQPSYSMLNRWIEEDLLDVLGD
                     EGVGCIAFSPLAQGMLTNKYLNGVPEGSRASQGKSLSPDLLTEQNLTHVRALNDIAEQ
                     RGQSLAQLALAWVLRDERVTSALIGASSVKQLEDSVGAVSNLSFSREELATIDAHAVE
                     GGINLWSGPGTA"
     gene            complement(1539..2507)
                     /locus_tag="PLF56_12185"
     CDS             complement(1539..2507)
                     /locus_tag="PLF56_12185"
                     /inference="COORDINATES: protein motif:HMM:NF013567.2"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_function: GO:0016491 - oxidoreductase activity
                     [Evidence IEA];
                     GO_function: GO:0016651 - oxidoreductase activity, acting
                     on NAD(P)H [Evidence IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="Gfo/Idh/MocA family oxidoreductase"
                     /protein_id="HOQ54356.1"
                     /translation="MVRFGIVGTNFISEWFAQACRDTNGRAEAVAVCSRDQARASAFA
                     QRIGAADGFDDLDRMADVVDAVYVATPVSAHHGQAVQAIGAGRHVLVEKTMGASAAEV
                     SDILAQATQQGVVAMEAVRNVHTPTHRLIGDALARVGEVRHVRFEKLQYSSRYDRFRA
                     GEVLNAFDPSLGNSALADIGVYCLQPALDLLGAPARTCGASVWLNNGFEAGGSLQLDY
                     GRAVADVVYSKVAAGSSPSVINGEDGWITLNDPAETSRVTFTGRDGRSEVLLDAGAIE
                     PGPTMVHEVCDFVDQVEAGETDVRWSDLSLLARRLMDEHLARGQHR"
BASE COUNT          473 a         1025 c          849 g          444 t
ORIGIN      
        1 acccgcgcct acacccaaac accccttgac gaaacatagg agagtcgcat gccgggatct
       61 ctgcccgcgc caagatcccg gggcaagccc gggatgacca atacgcggcg ccgggcatga
      121 cgagcacccc gcgccttgat catcgtctcc cgaggcctct ctcgtgaccc ggcaatcgtg
      181 gcggccgatc gtccggcata ggatcgaaac agacccgaag gagatcacct gatgccgctc
      241 gaacccgtct atgtacccgt ccccgaccgc tacgacggac gcatgcccta ccgccgcacc
      301 ggacgaagcg gcctgctgct gcctgccatc tcgctcggcc tgtggcacaa cttcggcgac
      361 gacgtgccgt tcgccacgca gcgcgccacc ctgcgccggg ccttcgatct gggtatcacc
      421 cacttcgatc tggccaacaa ctacggcccg ccctacggct cggctgaaac aaacttcgga
      481 cgccacctcc gcgacgattt cgcgccttac cgtgaccagt tggtcatctc gtccaaggcc
      541 ggctgggact tctggcccgg cccctacggc gacctcggct cgcgcaagta cctgatcggc
      601 tcgctcgatg cctccctgga gcgcatgggg ctggactacg tcgacatctt ctaccatcac
      661 cgcttcgacc ccgacacccc gctcgaagag accatgggtg cccttgacta cgccgttcgc
      721 tcgggaaggg ctcgctacat cggcatttcg tcgtactccg gcacgcgcac ccgcgaggcc
      781 gtggcgatcc tgcgcgagct cggcactccc ctgctcatcc accagccgtc gtactcgatg
      841 ctgaaccgct ggatcgagga agacctcttg gacgtcctgg gcgacgaggg cgtgggctgc
      901 atcgccttct caccactggc ccagggcatg ctcaccaaca agtacctgaa cggagtgccc
      961 gagggatcgc gggcttccca gggcaagtcg ctgtcgcccg acctgctgac ggagcagaac
     1021 ctgacccatg tgcgagccct caacgacatc gccgagcagc gcggccagag cctggcccag
     1081 ctcgcgctgg cctgggtgct gcgcgacgag cgggtgacgt ccgccctgat cggggcctcg
     1141 agcgtcaagc agctggagga cagcgtcggc gcagtgagca acctgtcgtt ctcgcgcgag
     1201 gaactcgcaa ccatcgatgc gcacgcggtc gagggcggca tcaacctgtg gtcggggccg
     1261 ggcaccgcgt gagcgaatcc ctcaaccgcc tcacctgctg atcaatacga ttcgttggtt
     1321 gagcctgtcg aaccccactc acgagggtct cggcacgctc gaccaacgat gatcgggctt
     1381 tcctgagccc ggatccacct cctctgcacg cgactcaacg ccgtccccat gttcgaggcc
     1441 gaccggcgcc aggaggacga cctccgacgc acttgcaacg attgcgccag ggttcgccgg
     1501 ccggaccctc tgcaccacag cgacagatcg gcgtttccct agcgatgctg cccgcgggcc
     1561 agatgctcgt ccatcaggcg ccgggccagc aggctcaggt cggaccagcg gacgtccgtt
     1621 tcgccggcct cgacctggtc gacgaaatcg cagacctcat gcaccatcgt gggacccggt
     1681 tcgatcgctc ctgcatcgag cagcacctcg ctgcggccgt cgcgcccggt gaacgtgacg
     1741 cgtgaggttt cggcagggtc gttgagcgtg atccagccgt cctcgccgtt gatcacgctg
     1801 gggctgctgc cggccgcgac cttggagtac accacgtcgg cgaccgcgcg cccgtagtcg
     1861 agttgcagac tgccgccggc ctcgaaaccg ttgttcagcc acacgctggc cccgcacgtg
     1921 cgcgctggag cacccagcag gtccagcgcc ggctgcaggc agtacacgcc gatgtcggcc
     1981 agggccgagt tgcccagcga cgggtcgaag gcgttcagca cctcgccggc ccggaaccgg
     2041 tcgtagcgag acgagtactg cagcttctcg aagcggacgt gccgcacttc gccgacccgc
     2101 gccagcgcgt ccccgatcag ccggtgggtc ggcgtgtgga cgttgcggac cgcctccatc
     2161 gcgaccacgc cctgctgcgt cgcctgggcc agaatgtcac tcacctcggc agcggaggcg
     2221 cccatggtct tctcgaccag cacgtggcgc ccggccccga tcgcctgcac agcctgtccg
     2281 tgatgcgccg ataccggggt ggccacgtac accgcatcca ccacgtcggc catccggtcg
     2341 aggtcgtcga acccgtccgc cgccccgatc cgctgtgcga aagcggacgc ccgggcctga
     2401 tcgcgtgaac agaccgccac cgcctcggcg cgtccgttcg tgtctcgaca cgcctgggcg
     2461 aaccactcgc tgatgaagtt ggttccgacg attccaaaac gcaccatcag tactcctccc
     2521 gccgcgcttc cggccgggta ggtcctcagc ccgacgaacg cggaatccca ccgggcaccg
     2581 tcccagccct acctggtgca gccctacctg gtgcaccgta gcgtgcccca acgtgcaacg
     2641 gcttcacgtc tgccggggaa aggggggcac tgacaggtcc tgtcgcgggc agaggcagcc
     2701 gcctatggtc gagatgtcac gtcatgagag gacgaacaat gcctgtgcag gtgcccaacg
     2761 tcacgctgaa caacggcgtc gagatgccga t
//
LOCUS       DAOSUG010000426         2782 bp    DNA     linear   ENV 10-JUN-2024
DEFINITION  MAG TPA_asm: Micropruina sp. isolate AMR_MDS_2195
            NODE_14639_length_2782_cov_4.322699, whole genome shotgun sequence.
ACCESSION   DAOSUG010000426 DAOSUG010000000
VERSION     DAOSUG010000426.1
DBLINK      BioProject: PRJNA925477
            BioSample: SAMN32864262
            Sequence Read Archive: SRR4236673
KEYWORDS    WGS; ENV; Metagenome Assembled Genome; MAG; Third Party Data; TPA;
            TPA:assembly.
SOURCE      Micropruina sp. (wastewater metagenome)
  ORGANISM  Micropruina sp.
            Bacteria; Bacillati; Actinomycetota; Actinomycetes;
            Propionibacteriales; Nocardioidaceae; Micropruina.
REFERENCE   1  (bases 1 to 2782)
  AUTHORS   Abdulkadir,N., Saraiva,J.P., Zhang,J., Stolte,S., Gillor,O.,
            Harms,H. and Rocha,U.
  TITLE     Genome-centric analyses of 165 metagenomes show that mobile genetic
            elements are crucial for the transmission of antimicrobial
            resistance genes to pathogens in activated sludge and wastewater
  JOURNAL   Microbiol Spectr 12 (3), e0291823 (2024)
   PUBMED   38289113
REFERENCE   2  (bases 1 to 2782)
  AUTHORS   Abdulkadir,N.
  TITLE     Direct Submission
  JOURNAL   Submitted (24-MAR-2023) Environmental Microbiology, Helmholtz
            Center for Environmental Research -UFZ, Permoserstr 15, Leipzig
            04318, Germany
COMMENT     The annotation was added by the NCBI Prokaryotic Genome Annotation
            Pipeline (PGAP). Information about PGAP can be found here:
            https://www.ncbi.nlm.nih.gov/genome/annotation_prok/
            
            ##Genome-Assembly-Data-START##
            Assembly Method        :: SPAdes v. 3.11.1
            Genome Representation  :: Full
            Expected Final Version :: Yes
            Genome Coverage        :: Not Applicable
            Sequencing Technology  :: Illumina MiSeq
            ##Genome-Assembly-Data-END##
            
            ##Genome-Annotation-Data-START##
            Annotation Provider               :: NCBI
            Annotation Date                   :: 05/04/2023 04:57:10
            Annotation Pipeline               :: NCBI Prokaryotic Genome
                                                 Annotation Pipeline (PGAP)
            Annotation Method                 :: Best-placed reference protein
                                                 set; GeneMarkS-2+
            Annotation Software revision      :: 6.5
            Features Annotated                :: Gene; CDS; rRNA; tRNA; ncRNA
            Genes (total)                     :: 2,776
            CDSs (total)                      :: 2,750
            Genes (coding)                    :: 2,714
            CDSs (with protein)               :: 2,714
            Genes (RNA)                       :: 26
            tRNAs                             :: 24
            ncRNAs                            :: 2
            Pseudo Genes (total)              :: 36
            CDSs (without protein)            :: 36
            Pseudo Genes (ambiguous residues) :: 0 of 36
            Pseudo Genes (frameshifted)       :: 2 of 36
            Pseudo Genes (incomplete)         :: 32 of 36
            Pseudo Genes (internal stop)      :: 2 of 36
            ##Genome-Annotation-Data-END##
FEATURES             Location/Qualifiers
     source          1..2782
                     /organism="Micropruina sp."
                     /mol_type="genomic DNA"
                     /submitter_seqid="NODE_14639_length_2782_cov_4.322699"
                     /isolate="AMR_MDS_2195"
                     /isolation_source="Activated sludge of municipal
                     wastewater treatment plants"
                     /db_xref="taxon:2737536"
                     /environmental_sample
                     /geo_loc_name="Canada"
                     /lat_lon="49.25 N 123.1 W"
                     /collection_date="2015-06-16"
                     /metagenome_source="wastewater metagenome"
                     /note="metagenomic"
     gene            complement(1..225)
                     /gene="atpE"
                     /locus_tag="PLF56_12190"
     CDS             complement(1..225)
                     /gene="atpE"
                     /locus_tag="PLF56_12190"
                     /EC_number="7.1.2.2"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_002526735.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_component: GO:0000276 - mitochondrial
                     proton-transporting ATP synthase complex, coupling factor
                     F(o) [Evidence IEA];
                     GO_component: GO:0045263 - proton-transporting ATP
                     synthase complex, coupling factor F(o) [Evidence IEA];
                     GO_component: GO:0045264 - plasma membrane
                     proton-transporting ATP synthase complex, coupling factor
                     F(o) [Evidence IEA];
                     GO_function: GO:0046933 - proton-transporting ATP synthase
                     activity, rotational mechanism [Evidence IEA];
                     GO_process: GO:0015986 - proton motive force-driven ATP
                     synthesis [Evidence IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="ATP synthase F0 subunit C"
                     /protein_id="HOQ54357.1"
                     /translation="MLPIMEISGSLNMIGYGLATIGPGIGVAMVFAAVINGTARQPEA
                     RGALMSTAWIGFAVVEALAIIGIALAFAIQ"
     gene            complement(260..1069)
                     /gene="atpB"
                     /locus_tag="PLF56_12195"
     CDS             complement(260..1069)
                     /gene="atpB"
                     /locus_tag="PLF56_12195"
                     /EC_number="7.1.2.2"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_013863899.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_component: GO:0000276 - mitochondrial
                     proton-transporting ATP synthase complex, coupling factor
                     F(o) [Evidence IEA];
                     GO_component: GO:0045263 - proton-transporting ATP
                     synthase complex, coupling factor F(o) [Evidence IEA];
                     GO_component: GO:0045264 - plasma membrane
                     proton-transporting ATP synthase complex, coupling factor
                     F(o) [Evidence IEA];
                     GO_function: GO:0046933 - proton-transporting ATP synthase
                     activity, rotational mechanism [Evidence IEA];
                     GO_process: GO:0015986 - proton motive force-driven ATP
                     synthesis [Evidence IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="F0F1 ATP synthase subunit A"
                     /protein_id="HOQ54358.1"
                     /translation="MGLLGLIIPMEGGEEGSHWPPGVHSFDPKPLFPDAGIDWLNTYL
                     LQAVIATILVIVLWLWVSKHQKVVPGFKQWCGEQLYNVVRNGIGRDALGPDYQKFTPY
                     LLALFSFILVNNLFGQFFLSMFPTFSRVGYAYALALLTWLLYNGVGIAKHGLGGYLKM
                     MTMPKGVPWPLYIIIIPLEILSNFIVRPVTLALRLFANLFAGHLVVIVFVVGGTLLLE
                     QSSFLYKIAGGASLLMAYPIFALEIFVGVLQAYIFTVLTAQYVASSLADDH"
     gene            complement(1089..1280)
                     /locus_tag="PLF56_12200"
     CDS             complement(1089..1280)
                     /locus_tag="PLF56_12200"
                     /inference="COORDINATES: protein motif:HMM:NF021073.2"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="AtpZ/AtpI family protein"
                     /protein_id="HOQ54359.1"
                     /translation="MEGGEGIDQGLRVLSYLLSGPLLYGFLGWLLDQWLHTSYWMVLG
                     IVFGMAAGVYLVIRRFGRP"
     gene            complement(1334..1687)
                     /locus_tag="PLF56_12205"
     CDS             complement(1334..1687)
                     /locus_tag="PLF56_12205"
                     /inference="COORDINATES: ab initio
                     prediction:GeneMarkS-2+"
                     /note="Derived by automated computational analysis using
                     gene prediction method: GeneMarkS-2+."
                     /codon_start=1
                     /transl_table=11
                     /product="hypothetical protein"
                     /protein_id="HOQ54360.1"
                     /translation="MAAWYLVGGPIAALNAALAAVLVLAFFGLGQAVQVWVADADPQV
                     VMIASLGSYVFRVGLPTLVLILLARDPSRFAGMHRVAIAVTAIVVVVGWLAAEIWTFS
                     RLRIPVFDPPEGESR"
     gene            complement(1775..>2782)
                     /locus_tag="PLF56_12210"
     CDS             complement(1775..>2782)
                     /locus_tag="PLF56_12210"
                     /EC_number="2.7.8.-"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_002513459.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_function: GO:0046872 - metal ion binding [Evidence
                     IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="MraY family glycosyltransferase"
                     /protein_id="HOQ54361.1"
                     /translation="AVAIVRDRDVHTRPIPYFGGIAMLCGLAAAFVVAGQLPWLGRFD
                     LVAHDSRAVFLAGLVICAVGVLDDMFDLNALAKIAGQMLAAGIVVLNGVKVYWIPLPS
                     TILTLDDGMSIVLTMALIFLCVNSINLIDGLDGLAAGVVAIGAGAFFSYAYWLSYEQE
                     LVRATTASLITVVIVGMCLGFLPYNVHPARMFMGDSGSMLLGFLLATSMISLTGQLDP
                     SQVTLQGSGLAINAYLPLFLPLAVMALPLGDLVLAYVRRTIAGRLWFQADKQHLHHRM
                     MQLGHSHARAVALLWLWTALLAFGSLMIGWTTSGWAIAALVAGIAVAAWLTWKPVRRA
                     T"
BASE COUNT          576 a          914 c          914 g          378 t
ORIGIN      
        1 tcactgaatg gcgaaggcga gggcgatgcc gatgatggcc agcgcctcga ccacggcgaa
       61 gccgatccag gcggtgctca tcagggcgcc gcgcgcctcg ggctggcggg cggtgccgtt
      121 gatcacggcg gcgaacacca ttgcgacgcc gatgccgggg ccgatggtgg ccaggccgta
      181 gccgatcatg ttgagcgagc cggagatctc catgatgggg agcattacgt ttcctttcgg
      241 agtttggtca aggtgggggt cagtggtcgt cggccaggga cgaggcaacg tactgagcgg
      301 tcagcacggt gaagatgtac gcctgcagca cgcccacgaa gatttcgagg gcgaagatcg
      361 ggtaggccat cagcagcgaa gcgccgccgg cgatcttgta cagaaagctg ctctgttcca
      421 gtagcagggt gccgccgacc acgaacacga tcaccacgag gtgaccggcg aacaggttgg
      481 cgaacagacg cagggccagg gtgaccggcc gcacgatgaa attggagaga atctcgagcg
      541 gaatgatgat gatgtacagc ggccacggca cgcccttcgg catggtcatc atcttcaggt
      601 agccgcccag cccgtgcttg gcgataccga cgccgttgta cagcagccag gtcagcagcg
      661 ccagcgcgta ggcgtaaccg acgcgcgaaa acgtcgggaa catgctcaag aagaactggc
      721 cgaacaggtt gttgaccaga atgaacgaga acagggcaag caggtacggg gtgaacttct
      781 ggtaatcggg gcccagtgcg tcacggccga tgccgttgcg caccacgttg tacaactgct
      841 caccgcacca ctgcttgaag ccgggcacga ctttctggtg tttcgagacc cacagccaca
      901 acacgatgac gaggatcgtg gcgatcacgg cctgcagcag gtacgtgttc aaccagtcga
      961 tgccggcatc gggaaacagt ggcttgggat cgaagctgtg cacaccgggg ggccagtgcg
     1021 agccctcctc gccgccctcc atcgggatga tcaggccgag cagccccatt gcacctcctg
     1081 cgtcgacgtc acggtcgtcc aaaccttctg atcaccaagt acaccccggc tgccattccg
     1141 aacacgatgc ccagcaccat ccagtaggac gtatgcagcc actgatcgag tagccagccg
     1201 agaaagccgt agagcaacgg acccgacaac aagtagctca ggacccgtag accctgatcg
     1261 atgccctcgc cgccctccat gactttcggt gactctagca gcaacggctc ggccagagcc
     1321 aatgagaact gccctagcgg gactcgcctt cgggtgggtc gaaaaccgga atccgcagac
     1381 gcgagaacgt ccagatctcg gccgccaacc accccactac cacgacgatc gccgtcaccg
     1441 cgatcgccac gcggtgcatg cccgcgaacc gggacggatc cctggccagc agaatcagca
     1501 ccagggtcgg caggcccacc cggaagacgt acgagcccag ggatgcgatc atcacgacct
     1561 gcggatcggc gtcggcgacc cacacctgca ccgcctggcc gaggccgaag aaggcgagca
     1621 ccagcacggc ggcgagggcg gcgttgagtg ccgcgatcgg tccaccgacc aggtaccacg
     1681 ctgccacggc gatcagggcg gccgcgtgtc cccggccgca cccccggcca gcagccggcg
     1741 ggcgcgctgc gcgttggcgc tgagcggtcg gggctcaggt ggcacggcgc accggcttcc
     1801 aggtcagcca ggcggcgacg gcgatgcctg cgaccagcgc cgcgatggcc cagcccgagg
     1861 tcgtccagcc gatcatcagc gagccgaagg cgagcagcgc cgtccagagc cagagcaggg
     1921 cgacggcgcg ggcgtggctg tgacccagtt gcatcatgcg gtggtgcagg tgctgcttgt
     1981 cggcctgaaa ccacagccgc ccggcgatcg tgcgccgcac gtaggccagc accaggtcgc
     2041 ccaacggcag cgccatcacg gccagcggca ggaacaacgg caggtaggcg ttgatcgcca
     2101 ggcccgagcc ctgcagggtg acctgcgacg ggtccagctg accggtcagg ctgatcatcg
     2161 aggtggccag cagaaagccg agcagcatcg agccggaatc gcccatgaac atgcgggccg
     2221 gatgcacgtt gtagggcaga aagcccaggc acatgcccac gatcaccacc gtgatcaggc
     2281 tggccgtggt ggcccgcacc aactcctgct cataactcag ccagtaggcg tagctgaaga
     2341 aggccccggc accgatggcg accaccccgg ccgccagccc gtccaggccg tcgatcaggt
     2401 tgatcgagtt cacgcacaga aagatcagcg ccatggtcag cacgatcgac atgccgtcat
     2461 caagggtgag gatcgtgctg ggcagcggga tccagtacac cttgacgccg ttgagcacga
     2521 cgatgccggc ggccagcatc tgcccggcga tcttggccag cgcgttcagg tcgaacatgt
     2581 cgtccagcac cccgacggcg cagatcacca ggccggccag aaacaccgcc cggctgtcgt
     2641 gcgccaccag gtcgaagcgg cccagccacg gcagctgccc ggccaccacg aacgccgcgg
     2701 ccaggccgca cagcatcgcg atgccgccga aatagggaat cggacgggtg tgcacgtcgc
     2761 ggtcacgcac gatggcgacg gc
//
LOCUS       DAOSUG010000427         2776 bp    DNA     linear   ENV 10-JUN-2024
DEFINITION  MAG TPA_asm: Micropruina sp. isolate AMR_MDS_2195
            NODE_14682_length_2776_cov_2.481808, whole genome shotgun sequence.
ACCESSION   DAOSUG010000427 DAOSUG010000000
VERSION     DAOSUG010000427.1
DBLINK      BioProject: PRJNA925477
            BioSample: SAMN32864262
            Sequence Read Archive: SRR4236673
KEYWORDS    WGS; ENV; Metagenome Assembled Genome; MAG; Third Party Data; TPA;
            TPA:assembly.
SOURCE      Micropruina sp. (wastewater metagenome)
  ORGANISM  Micropruina sp.
            Bacteria; Bacillati; Actinomycetota; Actinomycetes;
            Propionibacteriales; Nocardioidaceae; Micropruina.
REFERENCE   1  (bases 1 to 2776)
  AUTHORS   Abdulkadir,N., Saraiva,J.P., Zhang,J., Stolte,S., Gillor,O.,
            Harms,H. and Rocha,U.
  TITLE     Genome-centric analyses of 165 metagenomes show that mobile genetic
            elements are crucial for the transmission of antimicrobial
            resistance genes to pathogens in activated sludge and wastewater
  JOURNAL   Microbiol Spectr 12 (3), e0291823 (2024)
   PUBMED   38289113
REFERENCE   2  (bases 1 to 2776)
  AUTHORS   Abdulkadir,N.
  TITLE     Direct Submission
  JOURNAL   Submitted (24-MAR-2023) Environmental Microbiology, Helmholtz
            Center for Environmental Research -UFZ, Permoserstr 15, Leipzig
            04318, Germany
COMMENT     The annotation was added by the NCBI Prokaryotic Genome Annotation
            Pipeline (PGAP). Information about PGAP can be found here:
            https://www.ncbi.nlm.nih.gov/genome/annotation_prok/
            
            ##Genome-Assembly-Data-START##
            Assembly Method        :: SPAdes v. 3.11.1
            Genome Representation  :: Full
            Expected Final Version :: Yes
            Genome Coverage        :: Not Applicable
            Sequencing Technology  :: Illumina MiSeq
            ##Genome-Assembly-Data-END##
            
            ##Genome-Annotation-Data-START##
            Annotation Provider               :: NCBI
            Annotation Date                   :: 05/04/2023 04:57:10
            Annotation Pipeline               :: NCBI Prokaryotic Genome
                                                 Annotation Pipeline (PGAP)
            Annotation Method                 :: Best-placed reference protein
                                                 set; GeneMarkS-2+
            Annotation Software revision      :: 6.5
            Features Annotated                :: Gene; CDS; rRNA; tRNA; ncRNA
            Genes (total)                     :: 2,776
            CDSs (total)                      :: 2,750
            Genes (coding)                    :: 2,714
            CDSs (with protein)               :: 2,714
            Genes (RNA)                       :: 26
            tRNAs                             :: 24
            ncRNAs                            :: 2
            Pseudo Genes (total)              :: 36
            CDSs (without protein)            :: 36
            Pseudo Genes (ambiguous residues) :: 0 of 36
            Pseudo Genes (frameshifted)       :: 2 of 36
            Pseudo Genes (incomplete)         :: 32 of 36
            Pseudo Genes (internal stop)      :: 2 of 36
            ##Genome-Annotation-Data-END##
FEATURES             Location/Qualifiers
     source          1..2776
                     /organism="Micropruina sp."
                     /mol_type="genomic DNA"
                     /submitter_seqid="NODE_14682_length_2776_cov_2.481808"
                     /isolate="AMR_MDS_2195"
                     /isolation_source="Activated sludge of municipal
                     wastewater treatment plants"
                     /db_xref="taxon:2737536"
                     /environmental_sample
                     /geo_loc_name="Canada"
                     /lat_lon="49.25 N 123.1 W"
                     /collection_date="2015-06-16"
                     /metagenome_source="wastewater metagenome"
                     /note="metagenomic"
     gene            150..968
                     /locus_tag="PLF56_12215"
     CDS             150..968
                     /locus_tag="PLF56_12215"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_016455140.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_function: GO:0005524 - ATP binding [Evidence IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="ATP-binding cassette domain-containing protein"
                     /protein_id="HOQ54362.1"
                     /translation="MIRFQDVCKSYPDGTEAVRDFTLEAPTGKTTVLVGSSGCGKTTL
                     LRMVNRMVDPTSGAVWIDDENVADVPPVPLRRRIGYVMQNAGLLPHRKVIDNIATVPL
                     LNGVPKAQAHARAAELMELVGLEARQANRYPDQLSGGQQQRVGVARGLAADPNILLMD
                     EPFGAVDPLVRADLQAELLRIQRELEKTIVFVTHDIAEALALGDQIVILAKGARIAQA
                     GTPQQVVAEPADDFVADFLGLGRGTLDLHLRETPTGTLVVDGHGRPAGRLVEPG"
     gene            968..1606
                     /locus_tag="PLF56_12220"
     CDS             968..1606
                     /locus_tag="PLF56_12220"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_014845849.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_component: GO:0016020 - membrane [Evidence IEA];
                     GO_process: GO:0055085 - transmembrane transport [Evidence
                     IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="ABC transporter permease subunit"
                     /protein_id="HOQ54363.1"
                     /translation="MTWVLNNLELIGSRTLSHLLLAIPAIVASFVLALPLGWVANRYR
                     WSRATLLTAAGLLYAIPSLPLFVVLPSVLGFGVRDRINVIVALTLYGLALMVRSAADA
                     LASVGMPVVLSSTALGYSAAGRFFAVELPLAGPVLLAGLRVVSVSTISLVTVSSLLGV
                     DNLGLLFVDGFQRGIIAEVITGIVATVVLALIVDGLLVLLGRLAMPWTAVRR"
     gene            1603..2265
                     /locus_tag="PLF56_12225"
     CDS             1603..2265
                     /locus_tag="PLF56_12225"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_017934765.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_function: GO:0042626 - ATPase-coupled transmembrane
                     transporter activity [Evidence IEA];
                     GO_function: GO:0140359 - ABC-type transporter activity
                     [Evidence IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="ABC transporter permease"
                     /protein_id="HOQ54364.1"
                     /translation="MNYFTQALAWIADSANASGSNGIWARSWEQLWYTLLAVLLASMI
                     AIPLGYLVGHTGRGRGLVVASSGAARALPTLGVVTLLGLIFGIGLFAPLVALVLLAVP
                     SVLAGAYAGVEAIDPRTVDAARAMGMSEWQVLMRVEMPLGLPLLIGGLRSATLQVVAT
                     ATLAAYVGAGGLGRFLFYGLKTQDYPQMLAASMLVVAIALIMEGVFEIAERLVARRLR
                     RT"
     gene            2299..>2776
                     /locus_tag="PLF56_12230"
     CDS             2299..>2776
                     /locus_tag="PLF56_12230"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_016668086.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_component: GO:0043190 - ATP-binding cassette (ABC)
                     transporter complex [Evidence IEA];
                     GO_function: GO:0022857 - transmembrane transporter
                     activity [Evidence IEA];
                     GO_process: GO:0055085 - transmembrane transport [Evidence
                     IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="glycine betaine ABC transporter
                     substrate-binding protein"
                     /protein_id="HOQ54365.1"
                     /translation="MKTRIAAALAAGLLALSACSSANPLSSASPAASGSPTATGATTL
                     VVGSQQYYSNEIIAELYAQALEHHGFTITRQFQIGAREVYLPELKAGKIDVFPEYAGN
                     LLRALDKTSKAATSEEVLAALPKALGDNLRPLTAAPATDQDSFNVTGDFAKTNNVAS"
BASE COUNT          379 a          960 c          962 g          475 t
ORIGIN      
        1 ggccacgccg ttgctcaggt cgtagtgcgc gatgccggcc agggcaccgt ccacgtgaat
       61 ctcgaaacgt gactttcggg cgttgtgttc gacggtttca cccacagggt gagtatacga
      121 atcgggccgt ggtggggttg agtagggcaa tgatccgatt tcaggacgtg tgcaagtcgt
      181 accccgacgg caccgaggcc gtgcgcgact tcactctcga ggcgcccacg ggcaagacca
      241 ccgtgctggt cggatcgtcc ggctgcggca aaaccaccct gctgcgcatg gtgaaccgca
      301 tggtcgaccc gaccagcggt gcggtgtgga tcgacgacga gaacgtggcg gacgttccac
      361 cggtgccgtt gcgccggcgc atcgggtacg tgatgcagaa cgcgggcctg ctgccgcacc
      421 gcaaggtgat cgacaacatc gccaccgtgc cgctgctgaa cggcgtgccg aaggcgcagg
      481 cgcacgcccg ggcggccgaa ctgatggagc tggtcgggct cgaggcccgc caggccaacc
      541 gctaccccga ccagctctcg ggcggccagc agcagcgggt cggcgtggcc cgcgggctcg
      601 ccgccgaccc caacattctg ctgatggacg aaccgttcgg cgccgtcgac cccttggtgc
      661 gggccgacct gcaggccgaa ctgctgcgca ttcagcgcga gctcgagaag accatcgtgt
      721 tcgtcaccca cgacatcgcc gaggcgttgg ccctcggcga ccagatcgtg atcctggcca
      781 agggcgcccg catcgcgcag gccggtactc cgcagcaggt ggtggccgag ccggcggacg
      841 acttcgtggc cgacttcctg ggtctcggcc gcggcaccct cgacctgcac ctgcgcgaga
      901 ccccgaccgg cactctcgtg gtggacggcc acggacgtcc ggccggccgg ctggtggagc
      961 ccggctgatg acctgggtgc tcaacaacct cgagctgatc gggtcgcgca cgctgtcgca
     1021 cctgctgctc gcgattccgg cgatcgtcgc ctcgttcgtg ctggcgctgc cgctgggctg
     1081 ggtggccaac cggtaccgct ggtcgcgggc caccctgctg accgccgctg ggctgctgta
     1141 cgcgattccg tcgctgccgc tgttcgtggt gctgccctcg gtactcggct tcggggtgcg
     1201 cgaccgcatc aacgtgatcg tggcgctcac cctgtacggc ctggcgctga tggtgcgctc
     1261 ggccgccgac gccctggcgt cggtcggcat gccggtggtg ctgtcgtcca cagctctggg
     1321 ctactcggcc gccggacggt tcttcgccgt cgagctgcca ctggccggcc cggtgctgct
     1381 ggccgggctg cgggtggtgt cggtgagcac gatcagcctg gtcacggtga gttcgctgct
     1441 gggcgtcgac aacctgggcc tgctgttcgt ggacggcttt cagcgcggca tcatcgccga
     1501 ggtgatcacc ggcatcgtcg ccaccgtggt gctggccctg atcgtggacg gcctgctggt
     1561 gctgctgggc cggctggcga tgccctggac ggcggtccgc cggtgaatta cttcacgcag
     1621 gcgctggcgt ggatcgccga ctcggccaac gcgtccggct ccaacggcat ctgggcccgc
     1681 tcatgggagc aactctggta caccctgctc gcggtgctgc tggcctcgat gatcgccata
     1741 ccgctggggt atttggtcgg ccacaccgga cgtggccgcg ggctggtggt ggcctcgtcc
     1801 ggggcggccc gggcgctgcc cacgctgggc gtggtgaccc tgctcgggct gatcttcggc
     1861 atcggcctgt tcgcaccgct ggtggcgttg gtgctgctcg ccgttccctc ggtgctggcc
     1921 ggcgcctatg ccggtgtcga ggcgatcgac ccgcgcaccg tcgatgccgc gcgcgccatg
     1981 ggcatgtcgg agtggcaggt gctgatgcgg gtcgagatgc cgctcgggct gccgctgctg
     2041 atcggggggt tgcgttcggc gacccttcag gtggtggcca ccgccaccct ggccgcctac
     2101 gtgggggccg gcggcctggg acgctttctg ttctacggcc tcaaaaccca ggattatccc
     2161 cagatgctgg ccgcctcgat gctggtggtg gcgatcgcgt tgatcatgga aggagttttc
     2221 gagatcgccg agcgtctggt tgcccgacgc ctgcgcagaa cctgacatcc gctggacccg
     2281 caacgataag gaaacccgat gaagacacgc atcgccgccg cgctcgcggc cggcctgttg
     2341 gcgctcagcg cctgctcctc cgccaacccc ctgagctcgg ccagcccggc ggcgtccggc
     2401 tcgcccaccg cgaccggtgc caccacgctg gtggtcggct cgcagcagta ctactccaac
     2461 gagatcatcg ccgagttgta cgcgcaggcg ctcgaacacc acggcttcac catcacccgg
     2521 cagttccaga tcggcgcccg tgaggtgtac ctgcccgagc tgaaggccgg caagatcgac
     2581 gtgttccccg agtacgcggg caacctgctg cgggccctcg acaagacctc gaaggcggcc
     2641 accagcgaag aggtgctggc cgcgctgccc aaggcgttgg gcgacaacct gcgtccgctc
     2701 accgccgcgc ccgccaccga ccaggactcg ttcaacgtga ccggcgactt cgccaagacc
     2761 aacaatgtgg ccagcc
//
LOCUS       DAOSUG010000428         2773 bp    DNA     linear   ENV 10-JUN-2024
DEFINITION  MAG TPA_asm: Micropruina sp. isolate AMR_MDS_2195
            NODE_14704_length_2773_cov_4.071008, whole genome shotgun sequence.
ACCESSION   DAOSUG010000428 DAOSUG010000000
VERSION     DAOSUG010000428.1
DBLINK      BioProject: PRJNA925477
            BioSample: SAMN32864262
            Sequence Read Archive: SRR4236673
KEYWORDS    WGS; ENV; Metagenome Assembled Genome; MAG; Third Party Data; TPA;
            TPA:assembly.
SOURCE      Micropruina sp. (wastewater metagenome)
  ORGANISM  Micropruina sp.
            Bacteria; Bacillati; Actinomycetota; Actinomycetes;
            Propionibacteriales; Nocardioidaceae; Micropruina.
REFERENCE   1  (bases 1 to 2773)
  AUTHORS   Abdulkadir,N., Saraiva,J.P., Zhang,J., Stolte,S., Gillor,O.,
            Harms,H. and Rocha,U.
  TITLE     Genome-centric analyses of 165 metagenomes show that mobile genetic
            elements are crucial for the transmission of antimicrobial
            resistance genes to pathogens in activated sludge and wastewater
  JOURNAL   Microbiol Spectr 12 (3), e0291823 (2024)
   PUBMED   38289113
REFERENCE   2  (bases 1 to 2773)
  AUTHORS   Abdulkadir,N.
  TITLE     Direct Submission
  JOURNAL   Submitted (24-MAR-2023) Environmental Microbiology, Helmholtz
            Center for Environmental Research -UFZ, Permoserstr 15, Leipzig
            04318, Germany
COMMENT     The annotation was added by the NCBI Prokaryotic Genome Annotation
            Pipeline (PGAP). Information about PGAP can be found here:
            https://www.ncbi.nlm.nih.gov/genome/annotation_prok/
            
            ##Genome-Assembly-Data-START##
            Assembly Method        :: SPAdes v. 3.11.1
            Genome Representation  :: Full
            Expected Final Version :: Yes
            Genome Coverage        :: Not Applicable
            Sequencing Technology  :: Illumina MiSeq
            ##Genome-Assembly-Data-END##
            
            ##Genome-Annotation-Data-START##
            Annotation Provider               :: NCBI
            Annotation Date                   :: 05/04/2023 04:57:10
            Annotation Pipeline               :: NCBI Prokaryotic Genome
                                                 Annotation Pipeline (PGAP)
            Annotation Method                 :: Best-placed reference protein
                                                 set; GeneMarkS-2+
            Annotation Software revision      :: 6.5
            Features Annotated                :: Gene; CDS; rRNA; tRNA; ncRNA
            Genes (total)                     :: 2,776
            CDSs (total)                      :: 2,750
            Genes (coding)                    :: 2,714
            CDSs (with protein)               :: 2,714
            Genes (RNA)                       :: 26
            tRNAs                             :: 24
            ncRNAs                            :: 2
            Pseudo Genes (total)              :: 36
            CDSs (without protein)            :: 36
            Pseudo Genes (ambiguous residues) :: 0 of 36
            Pseudo Genes (frameshifted)       :: 2 of 36
            Pseudo Genes (incomplete)         :: 32 of 36
            Pseudo Genes (internal stop)      :: 2 of 36
            ##Genome-Annotation-Data-END##
FEATURES             Location/Qualifiers
     source          1..2773
                     /organism="Micropruina sp."
                     /mol_type="genomic DNA"
                     /submitter_seqid="NODE_14704_length_2773_cov_4.071008"
                     /isolate="AMR_MDS_2195"
                     /isolation_source="Activated sludge of municipal
                     wastewater treatment plants"
                     /db_xref="taxon:2737536"
                     /environmental_sample
                     /geo_loc_name="Canada"
                     /lat_lon="49.25 N 123.1 W"
                     /collection_date="2015-06-16"
                     /metagenome_source="wastewater metagenome"
                     /note="metagenomic"
     gene            complement(<1..1588)
                     /gene="drmD"
                     /locus_tag="PLF56_12235"
     CDS             complement(<1..1588)
                     /gene="drmD"
                     /locus_tag="PLF56_12235"
                     /inference="COORDINATES: protein motif:HMM:NF038317.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_function: GO:0005524 - ATP binding [Evidence IEA];
                     GO_function: GO:0140658 - ATP-dependent chromatin
                     remodeler activity [Evidence IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="DISARM system SNF2-like helicase DrmD"
                     /protein_id="HOQ54366.1"
                     /translation="MTPTAADMRELPELPTPGRVVEVRGSTWAVTDVVAQGLLRSPAD
                     ESVAGRQHVVALQSLDEDRLGEELRVIWELEVGHTLAPDRGLPVAIQAEAFDDPDTLG
                     AFVDAVRWGAVTSADDDAYQSPFRSGVNIHAYQLEPLRRALQSPRTNLLLADDVGLGK
                     TIEAGLVIQELLLRHRARTVIIVCPPSLSLKWQDEMREKFGLDFVIVNSERMAEVRRQ
                     HGLNANPLKVFPRVIVSMAWIASPRAQRLLRDVYADADARTTARRYAFDLLVVDEAHH
                     VAPAAPTPTGGNRGYAVDSLRTIHTRELAGRCEHRLFLTATPHNGHTESFTALMEMID
                     NRRFSRGADLDEEALRQVAVRRLKTELPELDFKARRVIPLPFEPDDAEQFRFDELSRI
                     IDASRKASGPDSGGDLVGLLLKKRFLSSPWSFGRTLARLADSASPPSGTADLFDDDAE
                     ALRELVGSEASDEEEGLEAQPEFAAMRRRQVRRALSTATLTQLNALAVWGLGFEATAD
                     SRLQALIDWLDGVCRIGDTWS"
     gene            1619..>2773
                     /locus_tag="PLF56_12240"
     CDS             1619..>2773
                     /locus_tag="PLF56_12240"
                     /inference="COORDINATES: protein motif:HMM:NF017885.2"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="XRE family transcriptional regulator"
                     /protein_id="HOQ54367.1"
                     /translation="MRLLNHVGDSDDEHSRSHCDDRSPVLRLSQDASTVGCMTTTAER
                     VRTLIEQRGHTQVAFAAEIGLDPTKLSKSLSGVRRFSSLDLALIADACAVTVDWLVTG
                     EDPPLAAAARASVGSAAGEALALARRYSTVRADLVDLGYPQGWRLPAAPRLGGLWKDQ
                     GAALAESALAQVGEVGALLADPLPNLIEQAFGIDVAVQPLGDGFDGLAVATDRLKLVL
                     AAPQPVPARLRFTIAHELGHLLASDDQQIHTDQNIYRTDRVESEVRANAFAATFLMPE
                     QLLRDRVQRGFDKKQFCALATTLRVSPMALAIRLEGLRLIDAGGREQWARLSGSEAAR
                     AAGLQSLNGQLATESLSIRPPGLLLRDAWAAYENGDITLRLVAGLAGSTPD"
BASE COUNT          446 a          898 c          959 g          470 t
ORIGIN      
        1 tcgaccacgt atcgccgatg cggcagacgc cgtccagcca atcgatcagt gcctgcaggc
       61 gcgaatcggc cgtggcctcg aagcccagcc cccagacagc caatgcgttg agctgggtca
      121 gggtcgcggt ggacagggcg cggcgaacct gacgtcggcg catcgccgcg aactcgggct
      181 gcgcttcgag gccctcctct tcgtcgctcg cctcgctacc gaccagctca cgcagcgcct
      241 cggcgtcgtc atcgaacaga tcggcggtgc cagacggggg cgacgctgag tcggccaggc
      301 gagccagcgt gcggccgaac gaccaagggc tggacagaaa ccgcttcttc agcagcaggc
      361 cgaccaggtc gccgcctgag tcggggccgg aggccttgcg gctcgcgtcg atgatgcggc
      421 ttagttcgtc gaaacggaac tgttctgcgt cgtccggctc gaacggaagg ggaatgaccc
      481 ggcgggcctt gaagtccaac tcgggaagtt cggtcttcaa cctgcgtacg gcgacctggc
      541 gcaacgcctc ttcgtcgagg tcggctccac gtgagaaccg gcggttgtcg atcatctcca
      601 ttagagctgt gaacgactcg gtgtgcccgt tgtgcggggt ggcggtcaga aaaagtcggt
      661 gttcgcagcg gccggcgagc tcgcgggtgt gaatcgtccg caggctgtcg accgcatagc
      721 cccggttgcc gccggtgggc gtcggtgcgg cgggagctac gtggtgggcc tcgtcgacca
      781 ccaacaggtc gaaggcgtag cgccgggcgg tggtgcgggc gtccgcgtcg gcgtagacgt
      841 cgcgcagcag ccgctgggcg cggggcgaag cgatccaggc catcgacacg atcactcgag
      901 ggaacacctt gagcgggttc gcgttgaggc cgtgctgccg gcgcacctcg gccatgcgtt
      961 cactgttcac gatgacgaag tcgaggccga acttctcgcg catctcgtcc tgccacttca
     1021 gcgagaggct cggcgggcag acaatgatca cggtgcgcgc gcggtgccgc agcagcaact
     1081 cctgaatgac caggccggcc tcgatggtct tgcccaaacc aacgtcgtcg gccagcagca
     1141 ggttcgtgcg cggtgattgc agggcgcgcc gtaacggctc cagttggtag gcgtgaatgt
     1201 tcaccccact gcggaaaggg gactggtagg cgtcgtcgtc cgccgaggtg acggctcccc
     1261 agcgcaccgc gtcgacgaac gctcctaggg tgtcggggtc gtcgaacgcc tcggcctgaa
     1321 tagcgacggg caggccccga tcgggagcca gcgtatgccc gacttccagc tcccagataa
     1381 cccgcagttc ttcgcctagt cgatcctcgt cgagcgactg cagggcgacg acgtgctgcc
     1441 gaccggcgac cgattcgtcg gcggggctgc gcaacagccc ctgagccacc acgtcggtga
     1501 ccgcccaggt ggagccgcgg acctccacca cgcggccggg tgtcggcaac tcgggcagct
     1561 cgcgcatgtc ggcagcagtc ggcgtcatag tgtcggagcc tcctgcttgg gtgggtggat
     1621 gaggttactc aaccatgtcg gtgacagcga cgacgagcat tcgcgaagcc attgcgatga
     1681 tcgtagtcct gtattgcgat tatcgcaaga tgcgtctacc gtagggtgta tgacgaccac
     1741 tgccgagcgg gtgcgcacac tcatcgaaca gcgagggcac acgcaggttg cgttcgccgc
     1801 tgagatcggg ttggacccaa ccaaactgtc gaagtcgctg tcgggcgtgc ggcggttcag
     1861 ctcgctcgac ttggcgctca tcgccgatgc ctgtgccgtg accgttgact ggctggtgac
     1921 tggcgaagat ccgcccctgg ctgcggcagc ccgcgcttct gtgggcagcg cggccggcga
     1981 ggcgctcgcc cttgcccgcc gctacagcac cgttcgagcc gacctggtcg acttgggcta
     2041 tccgcagggt tggcgattgc ccgccgcccc tcggctcgga gggctgtgga aagaccaagg
     2101 tgccgcgctg gccgagtcgg cgctggcaca ggtgggagag gtcggagccc tgctggccga
     2161 cccgctgccc aatctcatcg agcaggcgtt cggcatcgac gtcgccgtgc agccgctcgg
     2221 cgacggtttt gacggactgg cggtcgccac cgaccgcctg aagctggttc tcgcggcgcc
     2281 gcaaccggtg cccgcccgat tgcgtttcac catcgcccac gagctgggtc acctgctggc
     2341 cagcgatgac cagcagattc acaccgacca gaacatctac cgcaccgaca gggttgagag
     2401 tgaggtgcgc gccaacgcgt tcgctgccac gttcttgatg cctgagcagt tgctgcgcga
     2461 tcgagtgcaa aggggcttcg acaagaagca gttctgtgcg ttggccacca cccttcgcgt
     2521 ctcaccgatg gcactggcga tccggctgga agggttgcgc ctcatcgatg cgggcggtcg
     2581 tgagcaatgg gctcggttga gtggcagcga ggccgccagg gcggcgggat tgcagtccct
     2641 caacggtcaa ctggcgacgg agtcgttgtc cattcgtccg cccggcctgc tgctgcgtga
     2701 cgcctgggca gcctacgaga acggcgacat caccctccgg ctggtcgccg ggctggccgg
     2761 tagcaccccc gac
//
LOCUS       DAOSUG010000429         2771 bp    DNA     linear   ENV 10-JUN-2024
DEFINITION  MAG TPA_asm: Micropruina sp. isolate AMR_MDS_2195
            NODE_14721_length_2771_cov_4.169367, whole genome shotgun sequence.
ACCESSION   DAOSUG010000429 DAOSUG010000000
VERSION     DAOSUG010000429.1
DBLINK      BioProject: PRJNA925477
            BioSample: SAMN32864262
            Sequence Read Archive: SRR4236673
KEYWORDS    WGS; ENV; Metagenome Assembled Genome; MAG; Third Party Data; TPA;
            TPA:assembly.
SOURCE      Micropruina sp. (wastewater metagenome)
  ORGANISM  Micropruina sp.
            Bacteria; Bacillati; Actinomycetota; Actinomycetes;
            Propionibacteriales; Nocardioidaceae; Micropruina.
REFERENCE   1  (bases 1 to 2771)
  AUTHORS   Abdulkadir,N., Saraiva,J.P., Zhang,J., Stolte,S., Gillor,O.,
            Harms,H. and Rocha,U.
  TITLE     Genome-centric analyses of 165 metagenomes show that mobile genetic
            elements are crucial for the transmission of antimicrobial
            resistance genes to pathogens in activated sludge and wastewater
  JOURNAL   Microbiol Spectr 12 (3), e0291823 (2024)
   PUBMED   38289113
REFERENCE   2  (bases 1 to 2771)
  AUTHORS   Abdulkadir,N.
  TITLE     Direct Submission
  JOURNAL   Submitted (24-MAR-2023) Environmental Microbiology, Helmholtz
            Center for Environmental Research -UFZ, Permoserstr 15, Leipzig
            04318, Germany
COMMENT     The annotation was added by the NCBI Prokaryotic Genome Annotation
            Pipeline (PGAP). Information about PGAP can be found here:
            https://www.ncbi.nlm.nih.gov/genome/annotation_prok/
            
            ##Genome-Assembly-Data-START##
            Assembly Method        :: SPAdes v. 3.11.1
            Genome Representation  :: Full
            Expected Final Version :: Yes
            Genome Coverage        :: Not Applicable
            Sequencing Technology  :: Illumina MiSeq
            ##Genome-Assembly-Data-END##
            
            ##Genome-Annotation-Data-START##
            Annotation Provider               :: NCBI
            Annotation Date                   :: 05/04/2023 04:57:10
            Annotation Pipeline               :: NCBI Prokaryotic Genome
                                                 Annotation Pipeline (PGAP)
            Annotation Method                 :: Best-placed reference protein
                                                 set; GeneMarkS-2+
            Annotation Software revision      :: 6.5
            Features Annotated                :: Gene; CDS; rRNA; tRNA; ncRNA
            Genes (total)                     :: 2,776
            CDSs (total)                      :: 2,750
            Genes (coding)                    :: 2,714
            CDSs (with protein)               :: 2,714
            Genes (RNA)                       :: 26
            tRNAs                             :: 24
            ncRNAs                            :: 2
            Pseudo Genes (total)              :: 36
            CDSs (without protein)            :: 36
            Pseudo Genes (ambiguous residues) :: 0 of 36
            Pseudo Genes (frameshifted)       :: 2 of 36
            Pseudo Genes (incomplete)         :: 32 of 36
            Pseudo Genes (internal stop)      :: 2 of 36
            ##Genome-Annotation-Data-END##
FEATURES             Location/Qualifiers
     source          1..2771
                     /organism="Micropruina sp."
                     /mol_type="genomic DNA"
                     /submitter_seqid="NODE_14721_length_2771_cov_4.169367"
                     /isolate="AMR_MDS_2195"
                     /isolation_source="Activated sludge of municipal
                     wastewater treatment plants"
                     /db_xref="taxon:2737536"
                     /environmental_sample
                     /geo_loc_name="Canada"
                     /lat_lon="49.25 N 123.1 W"
                     /collection_date="2015-06-16"
                     /metagenome_source="wastewater metagenome"
                     /note="metagenomic"
     gene            complement(<1..1332)
                     /locus_tag="PLF56_12245"
     CDS             complement(<1..1332)
                     /locus_tag="PLF56_12245"
                     /inference="COORDINATES: protein motif:HMM:NF012235.2"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_function: GO:0005524 - ATP binding [Evidence IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="ATP-binding cassette domain-containing protein"
                     /protein_id="HOQ54368.1"
                     /translation="MSASLFLSGVAASFGARPLFAGLDLVLAPGDVTALVGPNGAGKT
                     TLLRIVAGEHAPDAGTVRFAPPDATLGYLPQSPPSATESILDYAADRTGVAAAQLQFE
                     SAAAALSSGDPEVADRYDRALAGWLALGGADLDVRLAEVVAGLGLRVALDRPLGSLSG
                     GQAARAALASILISRYDVLLLDEPTNNLDADGLAALTDFVQGLRSPVLIASHDRAFLD
                     AVATGVLELDAAQQQVNQYAGGWSDYRAAKSLTRQQAEQAYTDYAQERTALVEQARRK
                     TEWAGKGRARAAALGPGMKLEKKFREDKARRMDQRAARVRDAVERLDEVEQPRKEWEL
                     RFAITPAPASAEVVLTLDRVVVRNGDFVVGPITAQVVRGERVALVGPNGSGKTTLLQA
                     VLGRRQATSGRVGWGARVELGVLDQARGEIAGERPLLEVVAGPDTDPAVVER"
     gene            1588..2184
                     /locus_tag="PLF56_12250"
     CDS             1588..2184
                     /locus_tag="PLF56_12250"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_020386180.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_function: GO:0003677 - DNA binding [Evidence IEA];
                     GO_function: GO:0003700 - DNA-binding transcription factor
                     activity [Evidence IEA];
                     GO_process: GO:0006355 - regulation of DNA-templated
                     transcription [Evidence IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="TetR/AcrR family transcriptional regulator"
                     /protein_id="HOQ54369.1"
                     /translation="MARANPMTPDERRRALIEATRPLLLEHGPSVSTRMIAECADVAE
                     GTIFRAFGTKQALIEAVVDDCLSPEPLIEALRTIPHDLDLEDTVTWAISVLQTRTRQV
                     RSLMTALYPSDRTGPPRKPPHRHYHQVVNEALSATLSRFDDQLRVGSPTACWSITAMS
                     FASSMPFSDDPHTTDPRQVARLILHGIAAEPTTKDAPC"
     gene            2178..>2771
                     /locus_tag="PLF56_12255"
     CDS             2178..>2771
                     /locus_tag="PLF56_12255"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_020386179.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_component: GO:0016020 - membrane [Evidence IEA];
                     GO_function: GO:0005524 - ATP binding [Evidence IEA];
                     GO_function: GO:0042626 - ATPase-coupled transmembrane
                     transporter activity [Evidence IEA];
                     GO_process: GO:0055085 - transmembrane transport [Evidence
                     IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="ABC transporter transmembrane domain-containing
                     protein"
                     /protein_id="HOQ54370.1"
                     /translation="MLIRILREYLKPYSGQLAMVLLLQLIANAAALYLPTLNARIVDQ
                     GIATGNTGLILNVGALMLGITVVQMAAQIAAVWFGARSAMAFGRDLRAAIFDRTLDFS
                     AREVAKFGAPSLITRTTNDVQQVQQLVMMTSFMIIAAPIMMVGGIIMAFREDPGLTWT
                     IAVAVVILGAVIGLIISQMMPLFATQQIRIDTINRVLR"
BASE COUNT          444 a         1046 c          875 g          406 t
ORIGIN      
        1 ccgctcgacc acggctgggt cggtgtcggg gccggccacc acctcgagca gcggacgctc
       61 ccccgcgatc tcgccccggg cctgatcgag cacaccgagc tcgacccgcg caccccagcc
      121 gactcgtccc gatgtcgcct gacgtcgccc cagcaccgcc tgcagcagcg tggtcttgcc
      181 cgagccgttc ggcccgacca gtgccacccg ttcgccgcgc accacctgcg cggtgatcgg
      241 gcccacgacg aagtcaccgt tgcggaccac cacccgatcg agggtcagca ccacctctgc
      301 ggaggccggc gccggcgtga tcgcgaagcg caactcccac tccttgcgcg gctgctccac
      361 ctcgtcgagg cgctccacgg cgtcacggac gcgcgccgcc cgctggtcca tgcggcgcgc
      421 cttgtcctcg cggaacttct tctcgagctt catgcccggt cccagcgcgg cagccctggc
      481 ccggcccttg ccggcccatt cggtcttgcg ccgcgcctgt tcgaccagcg ccgtacgctc
      541 ctgcgcgtag tcggtatacg cctgctcggc ctgctgccgg gtcagcgact tcgccgcgcg
      601 gtagtcgctc cagccgccgg cgtactggtt cacctgctgc tgcgccgcgt cgagttcgag
      661 caccccggtt gccaccgcgt ccagaaacgc ccggtcgtgc gacgcgatca gcaccggcga
      721 gcgcagcccc tgcacgaagt cggtcagcgc cgccaacccg tcggcgtcga ggttgttggt
      781 cggctcatcc agtagcagca cgtcgtagcg gctgatcaga atggacgcca gcgcggcacg
      841 ggcagcctgc ccgccggaca atgacccgag cggacggtcc agggccacgc gcagcccgag
      901 cccggcgacc acctcggcca gccgcacgtc caggtcggcg ccgccgaggg cgagccagcc
      961 tgccaatgcc cggtcgtacc ggtcggccac ctcgggatcc cccgacgaca gcgccgcggc
     1021 cgcggactcg aactgcaact gcgccgcggc cacccccgtt cggtctgccg cgtagtcgag
     1081 aatcgactcc gtcgcggacg gcggcgactg cggcaggtag ccgagcgtgg cgtccggtgg
     1141 ggcgaaccga accgtgccgg cgtccggcgc gtgctcgccg gccacgatgc gcagcagggt
     1201 ggtcttgccg gcaccgttgg ggccgaccag agcggtgacg tcaccgggtg ccagcaccag
     1261 gtcgagcccg gcgaacaacg gacgagcgcc gaacgaagcg gcgacgccgg aaagaaacag
     1321 ggatgcggac atgacgagaa cctcgatcgc tggatgaagc agggcgagag gtcagttcca
     1381 catggctcca gggtgcggca cgccggcgac cgagtcaaac cgcttgatgc accgccggcg
     1441 ggggtctgcg gtgagcgacc ggcgcagcca gggtgcccgg atgggatcgc gcagcagggg
     1501 cagatcggtg gatccgggtt caggacgcgg gcagaaggtc gcgacgtcca cttgaatgag
     1561 tgagcactca ctcactacac tgtgcgggtg gcacgagcaa atcccatgac gcccgacgag
     1621 cggcgccgcg cgctgatcga ggcgacccgg ccattgttgc tcgaacacgg gccgagcgtc
     1681 agcacccgca tgatcgccga atgcgccgac gtggccgagg gcaccatctt tcgcgccttc
     1741 ggcaccaagc aggccctgat cgaggcggtg gtggacgact gcctgtcacc cgagccgctg
     1801 atcgaggcgc tgcggacgat cccgcacgac ctcgatctcg aagacaccgt cacctgggcg
     1861 atctcggtgt tgcagacccg cacccgccag gtgcgttcac tgatgacggc gctctacccc
     1921 agcgaccgca ccggcccgcc ccgcaagccg ccgcaccgcc attatcacca ggtggtgaac
     1981 gaggccctga gcgcgacgct ctctcgtttc gacgaccagt tgcgagtagg ctcaccaacc
     2041 gcctgctggt ccatcaccgc catgtccttc gcctcgagca tgccgttctc ggacgatccc
     2101 cacaccaccg atccgcgcca ggtcgctcgc ctgatcctgc acggaatcgc cgccgaaccc
     2161 accaccaagg acgccccatg ctgatccgca ttctgcgcga gtacctgaag ccctacagcg
     2221 gccagctcgc catggtgttg ctgctgcaac tcatcgccaa cgccgcggcc ctctatctac
     2281 ccacgttgaa cgcccgcatc gtcgaccagg gcatcgccac cggcaacacc gggctgatcc
     2341 tcaacgtcgg cgcgctgatg ctcggcatca ccgtggtgca gatggccgcc cagatcgccg
     2401 ccgtctggtt cggggcccgc tcggccatgg ccttcggacg cgacctgcgc gccgccatct
     2461 tcgaccgcac cctcgacttc tccgcccgcg aggtggccaa gttcggcgcc cccagcctga
     2521 tcacccgcac caccaacgac gtccagcagg tgcagcaact ggtgatgatg acgtcgttca
     2581 tgatcatcgc cgccccgatc atgatggtcg gcggcatcat catggcgttt cgcgaagatc
     2641 ccggactgac ctggacgatc gccgtcgccg tcgtcattct cggtgccgtg atcggcctga
     2701 tcatcagtca gatgatgccg ctgttcgcca cccagcagat ccgcatcgac accatcaacc
     2761 gggtgctgcg c
//
LOCUS       DAOSUG010000430         2766 bp    DNA     linear   ENV 10-JUN-2024
DEFINITION  MAG TPA_asm: Micropruina sp. isolate AMR_MDS_2195
            NODE_14744_length_2766_cov_3.995205, whole genome shotgun sequence.
ACCESSION   DAOSUG010000430 DAOSUG010000000
VERSION     DAOSUG010000430.1
DBLINK      BioProject: PRJNA925477
            BioSample: SAMN32864262
            Sequence Read Archive: SRR4236673
KEYWORDS    WGS; ENV; Metagenome Assembled Genome; MAG; Third Party Data; TPA;
            TPA:assembly.
SOURCE      Micropruina sp. (wastewater metagenome)
  ORGANISM  Micropruina sp.
            Bacteria; Bacillati; Actinomycetota; Actinomycetes;
            Propionibacteriales; Nocardioidaceae; Micropruina.
REFERENCE   1  (bases 1 to 2766)
  AUTHORS   Abdulkadir,N., Saraiva,J.P., Zhang,J., Stolte,S., Gillor,O.,
            Harms,H. and Rocha,U.
  TITLE     Genome-centric analyses of 165 metagenomes show that mobile genetic
            elements are crucial for the transmission of antimicrobial
            resistance genes to pathogens in activated sludge and wastewater
  JOURNAL   Microbiol Spectr 12 (3), e0291823 (2024)
   PUBMED   38289113
REFERENCE   2  (bases 1 to 2766)
  AUTHORS   Abdulkadir,N.
  TITLE     Direct Submission
  JOURNAL   Submitted (24-MAR-2023) Environmental Microbiology, Helmholtz
            Center for Environmental Research -UFZ, Permoserstr 15, Leipzig
            04318, Germany
COMMENT     The annotation was added by the NCBI Prokaryotic Genome Annotation
            Pipeline (PGAP). Information about PGAP can be found here:
            https://www.ncbi.nlm.nih.gov/genome/annotation_prok/
            
            ##Genome-Assembly-Data-START##
            Assembly Method        :: SPAdes v. 3.11.1
            Genome Representation  :: Full
            Expected Final Version :: Yes
            Genome Coverage        :: Not Applicable
            Sequencing Technology  :: Illumina MiSeq
            ##Genome-Assembly-Data-END##
            
            ##Genome-Annotation-Data-START##
            Annotation Provider               :: NCBI
            Annotation Date                   :: 05/04/2023 04:57:10
            Annotation Pipeline               :: NCBI Prokaryotic Genome
                                                 Annotation Pipeline (PGAP)
            Annotation Method                 :: Best-placed reference protein
                                                 set; GeneMarkS-2+
            Annotation Software revision      :: 6.5
            Features Annotated                :: Gene; CDS; rRNA; tRNA; ncRNA
            Genes (total)                     :: 2,776
            CDSs (total)                      :: 2,750
            Genes (coding)                    :: 2,714
            CDSs (with protein)               :: 2,714
            Genes (RNA)                       :: 26
            tRNAs                             :: 24
            ncRNAs                            :: 2
            Pseudo Genes (total)              :: 36
            CDSs (without protein)            :: 36
            Pseudo Genes (ambiguous residues) :: 0 of 36
            Pseudo Genes (frameshifted)       :: 2 of 36
            Pseudo Genes (incomplete)         :: 32 of 36
            Pseudo Genes (internal stop)      :: 2 of 36
            ##Genome-Annotation-Data-END##
FEATURES             Location/Qualifiers
     source          1..2766
                     /organism="Micropruina sp."
                     /mol_type="genomic DNA"
                     /submitter_seqid="NODE_14744_length_2766_cov_3.995205"
                     /isolate="AMR_MDS_2195"
                     /isolation_source="Activated sludge of municipal
                     wastewater treatment plants"
                     /db_xref="taxon:2737536"
                     /environmental_sample
                     /geo_loc_name="Canada"
                     /lat_lon="49.25 N 123.1 W"
                     /collection_date="2015-06-16"
                     /metagenome_source="wastewater metagenome"
                     /note="metagenomic"
     gene            <1..769
                     /locus_tag="PLF56_12260"
     CDS             <1..769
                     /locus_tag="PLF56_12260"
                     /inference="COORDINATES: protein motif:HMM:NF022794.2"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=2
                     /transl_table=11
                     /product="DUF3152 domain-containing protein"
                     /protein_id="HOQ54371.1"
                     /translation="GLDWTRIGLVIGAVAVLIAAVFGVRALSAPVEKAGAPTIAVPAP
                     TGTRFTSPSPSPSPTGTESGSASASASANASASPSSKATSSIKASGDFDWSTATVGAS
                     GSAGTLYRYVVGVESSAKLKANSVAGTVADVLNDPRSWTGDGDVRFALVGEAKAEVRI
                     QLASTKTAAALCGSGAQPTYTCVKGKTVVINAVQWKAAPASFAGDLDGYRTFLINHAV
                     GQLIGKSEAQCGGKGKKAPVMMQQGAGLNGCKANPWP"
     gene            complement(1039..1464)
                     /locus_tag="PLF56_12265"
     CDS             complement(1039..1464)
                     /locus_tag="PLF56_12265"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_016456367.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="cupin domain-containing protein"
                     /protein_id="HOQ54372.1"
                     /translation="MTVRKNKDHGKEPYVLDIEKRTLDNDNFRTTLWTGAHLQLTVMS
                     IPVGGDVGLEVHPKNDQFLRVEKGEGLVEMGPKKSELSFRKKVEDDYVILVPAGTWHN
                     VTNTGDEPLKLYALYGPADHEPGTVHPTQHDAETDPAED"
     gene            1653..>2766
                     /locus_tag="PLF56_12270"
     CDS             1653..>2766
                     /locus_tag="PLF56_12270"
                     /inference="COORDINATES: protein motif:HMM:NF014757.2"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="3-hydroxyacyl-CoA dehydrogenase family protein"
                     /protein_id="HOQ54373.1"
                     /translation="MNTRVRRAVVVGAGAMGSGIAAHLANAGVQVHLLDVVPEGESDD
                     RSRLAREAIERQVKAAGFMLSVFAERVTPGNVVDDVAAYAEADWVIEAVFEDLAVKRA
                     LYQVIEQHRGPGTIVTSNTSTIPLARLVEGRDDDFVRHFAITHFFNPPRQLPLLELVA
                     GPGTDPSVVEVLTAAGDHQLGKTVLRCRDTPGFLANRIGNFWMAVAAAEALRGEVDIE
                     TADTVMARHFGTPRTGVFGLFDLVGINLVPLIWTSFLKLLDDDDAYHRYDITADPTFT
                     GLLQRGLVGRRGPGGFTRRTKTAEGPLDEVIDTATFEYRPRRAPSDQVVQARGLRAAC
                     ETDSAGGRYAWSVLRELVDYCCTVAPQIADHVGLIDD"
BASE COUNT          439 a          913 c          955 g          459 t
ORIGIN      
        1 cggtctggac tggacccgga tcgggctcgt gatcggcgct gtcgcggttc tgatcgcggc
       61 cgtgttcggc gtgcgggcgc tgtccgcccc ggtcgagaag gccggggcac ccacgatcgc
      121 ggttcccgct cccacgggta cccgcttcac cagcccatcg ccctcgccga gtccgaccgg
      181 caccgaaagc ggctcggcgt ccgcgtcggc cagtgcgaac gcgagcgcgt cgccgtccag
      241 caaggcgacg agcagcatca aggcgtcagg cgatttcgac tggtcgacgg cgacggtggg
      301 tgcgtccgga tcggcgggca ccctgtaccg ctacgtggtc ggcgtggaga gttcggccaa
      361 gctgaaggcg aacagcgtcg cgggcaccgt tgccgacgtg ctcaacgacc cgcgcagctg
      421 gaccggcgac ggcgacgtcc gattcgcgct ggtcggcgaa gcgaaggccg aggtgcgcat
      481 ccaactggcg agtaccaaaa ccgcagcggc actgtgcggc tcgggcgcgc agccgaccta
      541 cacgtgtgtg aagggcaaga cggtggtgat caacgccgtg caatggaagg cggccccggc
      601 atcgttcgcg ggcgacctcg acggctatcg cacctttctg atcaaccacg ccgtcggtca
      661 actgatcggc aagagcgagg cgcagtgcgg cggcaagggc aagaaggcgc cggtgatgat
      721 gcaacagggc gccgggctga acggttgcaa agccaacccc tggccgtgag cggcgactca
      781 cccggtcacg cgatggccgg gtgaacgtgg atcagcgttt cccgaagatt cgtgaccccc
      841 gatatcccgg gaacgccggg gtctgtgcgt gcaccgagat cccggggcaa gcccgggatg
      901 acacgcacgt cccgtcattc cggtgaacgc cgggatctgt gcgcgcaccg atatcccggg
      961 gcaaacccga gtggacgagt acggcacttg tcgagacccc tactcgccag ggtctcgaca
     1021 agcgtggccg gcgatggatc agtcctcggc ggggtccgtt tcggcgtcgt gctgagtggg
     1081 gtggacggtg cccggctcat gatcggccgg cccgtacagc gcatacagct tcaacggctc
     1141 gtcaccggtg ttggtgacgt tgtgccaggt gccggcgggc accaggatca cgtagtcgtc
     1201 ctcgaccttc ttgcgaaagc tcagctcgct cttcttcgga cccatctcga ccaggccctc
     1261 acccttttcg acgcgcagga actggtcgtt cttcgggtgc acctcgaggc cgacgtcgcc
     1321 acccaccgga atggacatca cggtcagctg caggtgcgcg ccggtccaca gggtggtgcg
     1381 gaagttgtcg ttgtcgaggg ttctcttctc gatatcgagg acgtacggct ccttgccgtg
     1441 atccttgttc tttctgacgg tcatcacgtg ctcccttcga tccatcatgc ttaccgcatg
     1501 cagacccggt ggtcgatgca ttgagacaac ttctctcccc gaagggcgag tggcgagcga
     1561 cgcgctggct ggcgtgaggc gtcggacgac caggggtcgg acgcccgacc acactaggct
     1621 cgtcactatc cgaactcgac gaggagggtc tgatgaacac gcgggtgcgc agggcggtgg
     1681 tcgtcggtgc gggggccatg gggtcgggta tcgccgccca cctggccaac gcgggcgtgc
     1741 aggtgcacct gctcgatgtg gtgcccgagg gcgagtcgga cgaccgctca cgactcgcgc
     1801 gcgaggcgat cgagcgacag gtgaaggccg caggcttcat gctgtcggtg ttcgccgagc
     1861 gggtgacgcc cggcaacgtc gtggacgatg tcgccgccta tgccgaggcc gactgggtga
     1921 tcgaggcggt cttcgaagac ctggccgtca agcgcgcgtt gtatcaggtc atcgaacagc
     1981 accggggacc gggcacgatc gtgacctcca acacctcgac gatcccgctc gcccggctgg
     2041 tcgaggggcg cgacgacgac ttcgtgcggc actttgcgat cacccacttc ttcaatccgc
     2101 cccggcaact gccgctgctg gagctcgtgg ccgggccggg cacggacccg tccgtggtcg
     2161 aggtgttgac ggccgccggt gaccaccaac tgggcaagac ggtgctgcgc tgccgcgaca
     2221 ctccgggctt tctggccaac cggatcggca acttctggat ggccgttgcg gccgccgagg
     2281 cgttgcgcgg cgaggtcgac atcgagaccg ccgataccgt gatggccagg catttcggca
     2341 cgccgcgcac cggagtcttc gggctgttcg acctggtcgg catcaacctg gtgccgctga
     2401 tctggaccag ctttctcaag cttctggacg atgacgacgc ctaccaccgc tacgacatca
     2461 cggccgaccc gaccttcacc ggtctgctgc agcgtggtct ggtcggacgc cgcgggcccg
     2521 gcggcttcac ccgtcgcacc aagaccgccg agggtccgct cgacgaggtg atcgacaccg
     2581 ccaccttcga gtaccggccg cgccgcgccc cgtccgatca ggtggtgcag gcgcgcgggc
     2641 tgagggcggc ctgtgagacc gattcggccg gcggacgcta cgcctggtcg gtgctgcgcg
     2701 aactggtcga ctactgctgc accgtggcgc cccagatcgc cgaccacgtc ggcctgatcg
     2761 acgacg
//
LOCUS       DAOSUG010000431         2764 bp    DNA     linear   ENV 10-JUN-2024
DEFINITION  MAG TPA_asm: Micropruina sp. isolate AMR_MDS_2195
            NODE_14766_length_2764_cov_4.113695, whole genome shotgun sequence.
ACCESSION   DAOSUG010000431 DAOSUG010000000
VERSION     DAOSUG010000431.1
DBLINK      BioProject: PRJNA925477
            BioSample: SAMN32864262
            Sequence Read Archive: SRR4236673
KEYWORDS    WGS; ENV; Metagenome Assembled Genome; MAG; Third Party Data; TPA;
            TPA:assembly.
SOURCE      Micropruina sp. (wastewater metagenome)
  ORGANISM  Micropruina sp.
            Bacteria; Bacillati; Actinomycetota; Actinomycetes;
            Propionibacteriales; Nocardioidaceae; Micropruina.
REFERENCE   1  (bases 1 to 2764)
  AUTHORS   Abdulkadir,N., Saraiva,J.P., Zhang,J., Stolte,S., Gillor,O.,
            Harms,H. and Rocha,U.
  TITLE     Genome-centric analyses of 165 metagenomes show that mobile genetic
            elements are crucial for the transmission of antimicrobial
            resistance genes to pathogens in activated sludge and wastewater
  JOURNAL   Microbiol Spectr 12 (3), e0291823 (2024)
   PUBMED   38289113
REFERENCE   2  (bases 1 to 2764)
  AUTHORS   Abdulkadir,N.
  TITLE     Direct Submission
  JOURNAL   Submitted (24-MAR-2023) Environmental Microbiology, Helmholtz
            Center for Environmental Research -UFZ, Permoserstr 15, Leipzig
            04318, Germany
COMMENT     The annotation was added by the NCBI Prokaryotic Genome Annotation
            Pipeline (PGAP). Information about PGAP can be found here:
            https://www.ncbi.nlm.nih.gov/genome/annotation_prok/
            
            ##Genome-Assembly-Data-START##
            Assembly Method        :: SPAdes v. 3.11.1
            Genome Representation  :: Full
            Expected Final Version :: Yes
            Genome Coverage        :: Not Applicable
            Sequencing Technology  :: Illumina MiSeq
            ##Genome-Assembly-Data-END##
            
            ##Genome-Annotation-Data-START##
            Annotation Provider               :: NCBI
            Annotation Date                   :: 05/04/2023 04:57:10
            Annotation Pipeline               :: NCBI Prokaryotic Genome
                                                 Annotation Pipeline (PGAP)
            Annotation Method                 :: Best-placed reference protein
                                                 set; GeneMarkS-2+
            Annotation Software revision      :: 6.5
            Features Annotated                :: Gene; CDS; rRNA; tRNA; ncRNA
            Genes (total)                     :: 2,776
            CDSs (total)                      :: 2,750
            Genes (coding)                    :: 2,714
            CDSs (with protein)               :: 2,714
            Genes (RNA)                       :: 26
            tRNAs                             :: 24
            ncRNAs                            :: 2
            Pseudo Genes (total)              :: 36
            CDSs (without protein)            :: 36
            Pseudo Genes (ambiguous residues) :: 0 of 36
            Pseudo Genes (frameshifted)       :: 2 of 36
            Pseudo Genes (incomplete)         :: 32 of 36
            Pseudo Genes (internal stop)      :: 2 of 36
            ##Genome-Annotation-Data-END##
FEATURES             Location/Qualifiers
     source          1..2764
                     /organism="Micropruina sp."
                     /mol_type="genomic DNA"
                     /submitter_seqid="NODE_14766_length_2764_cov_4.113695"
                     /isolate="AMR_MDS_2195"
                     /isolation_source="Activated sludge of municipal
                     wastewater treatment plants"
                     /db_xref="taxon:2737536"
                     /environmental_sample
                     /geo_loc_name="Canada"
                     /lat_lon="49.25 N 123.1 W"
                     /collection_date="2015-06-16"
                     /metagenome_source="wastewater metagenome"
                     /note="metagenomic"
     gene            complement(<1..616)
                     /locus_tag="PLF56_12275"
     CDS             complement(<1..616)
                     /locus_tag="PLF56_12275"
                     /EC_number="1.6.5.9"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_002529861.1"
                     /note="part of NADH-ubiquinone oxidoreductase complex I;
                     shuttles electrons from NADH, via FMN and iron-sulfur
                     (Fe-S) centers, to quinones in the respiratory chain; NuoF
                     is part of the soluble NADH dehydrogenase fragment, which
                     represents the electron input part of NADH dehydrogenase;
                     Derived by automated computational analysis using gene
                     prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="NADH-quinone oxidoreductase subunit F"
                     /protein_id="HOQ54374.1"
                     /translation="MTDLLTPVLSENWADERAWKLTNYERRGGYRALRKALSTTPADV
                     MNTVKDSGLRGRGGAGFPTGMKWSFVPKDNPNPKYLVVNADESEPGTCKDMPLMLASP
                     HTLVEGVIISSYAIGCAYAFIYCRGEVLHVIRRLQQAVREAYSAGYAGKNILGSGYDL
                     EVVVHAGAGAYICGEETALLDSLEGRRGQPRLRPPFPAVAGLYAS"
     gene            complement(613..1386)
                     /gene="nuoE"
                     /locus_tag="PLF56_12280"
     CDS             complement(613..1386)
                     /gene="nuoE"
                     /locus_tag="PLF56_12280"
                     /EC_number="1.6.5.9"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_016668051.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_function: GO:0016491 - oxidoreductase activity
                     [Evidence IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="NADH-quinone oxidoreductase subunit NuoE"
                     /protein_id="HOQ54375.1"
                     /translation="MSGHEFQSYFPESGHIDYDDTSTNITETTLDEMRSIAARYPQAR
                     SALLPMLHLVQSVDGRVSPAGVQACADILGITTAQVSGVATFYTMYRRQPGGRNHVGV
                     CTTALCAVMGGDQLLDQVCHRLGIHEGETTADGAISLERLECNAACDFAPVMMVNWEF
                     FDNMDPRKVDELLDALSSGQSVHSPRGATITSWQEAERVLAGFPDGRADEGPTAGEAS
                     LLGLKVARERGWTAPEVPPPVPTSDEASANQSSPQGGAK"
     gene            complement(1383..2738)
                     /locus_tag="PLF56_12285"
     CDS             complement(1383..2738)
                     /locus_tag="PLF56_12285"
                     /EC_number="7.1.1.-"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_011754015.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_function: GO:0016651 - oxidoreductase activity, acting
                     on NAD(P)H [Evidence IEA];
                     GO_function: GO:0048038 - quinone binding [Evidence IEA];
                     GO_function: GO:0051287 - NAD binding [Evidence IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="NADH-quinone oxidoreductase subunit D"
                     /protein_id="HOQ54376.1"
                     /translation="MSTTDFYAQPGEETEGTVYTAMGGDWAEIAAEQAERSDEILVIN
                     MGPQHPSTHGVLRLILECDGETVLSIRPGIGFLHTGIEKNMEYRTWTQGSTFVTRMDY
                     VAPIFNEAAYCLAVERLLGIEADIPERATVIRVLMMELNRIGSHLVAIGTGGMEIGAL
                     TVMTIGFREREMILDFFEAVTGLRMNHAFIRPGGVANDLPETGIAQLRELIDWLHKHL
                     PEYAAFCNENPIFKMRMIGIGHQDLSACMALGVTGPVLRSTGYDWDLRKQQPYCGYET
                     YEFDSVAWRPEDGWASSDSYARFRIRLEEMWQSLRIVEQCADRLEASRGEPVMVADPK
                     IAWPAQLALGPDGQGNSHEHIKHIMGESMEALIHHFKIVTEGFRVPPGQAYVPIESPK
                     GEIACHVVSDGGTRPFRVHMRDPGFNHLQSVPIQCEGGMISDVVVAVGSLDPVMGGVD
                     R"
BASE COUNT          415 a          924 c          948 g          477 t
ORIGIN      
        1 gcgaggcgta caggccggcc acggccggga acggcggacg cagccggggc tggccccggc
       61 ggccctccag cgagtcgagc agtgcggtct cttcgccgca gatgtaggcg ccggcaccgg
      121 cgtgcacaac cacctcgagg tcgtaaccgc tgccgagaat gttcttgccc gcgtacccgg
      181 ccgaatacgc ctcacggacg gcctgctgca gccgccggat cacgtgcagc acctcgccgc
      241 gacagtagat gaacgcgtag gcgcagccga tcgcgtacga actgatgatc acgccttcga
      301 ccagcgtgtg cgggctggcc agcatcaacg gcatgtcttt gcaggtgccc ggctcggact
      361 cgtcggcgtt gaccaccagg tatttagggt tggggttgtc cttgggcacg aacgaccact
      421 tcatgccggt cggaaaaccg gcaccgccgc gtccacgcag gccggaatcc ttcaccgtgt
      481 tcatcacgtc ggccggggtg gtgctgagcg ccttgcgcag tgcccgatag ccgccgcggc
      541 gctcgtagtt ggtcagcttc caggcccgct cgtcggccca gttctcgctc agcacggggg
      601 tcaacaggtc ggtcacttcg cacctccttg cggggaactc tggttggcgg acgcctcgtc
      661 cgaggtcggc acgggcggcg gcacctcggg tgccgtccag ccgcgttcgc gggccacctt
      721 caaccccagc agggacgcct cgccggccgt cgggccttcg tcggcccgtc cgtccgggaa
      781 gccggcgagc acccgctcgg cttcctgcca ggaggtgatc gtggcacccc ggggcgagtg
      841 gacgctctgc ccggagctga gcgcgtccag cagctcgtcc accttgcggg gatccatgtt
      901 gtcgaagaac tcccagttga ccatcatcac cggggcgaag tcgcaggccg cgttgcattc
      961 gaggcgctcg agcgagatcg cgccgtcggc ggtggtctcg ccctcatgga tgccgagccg
     1021 gtggcagacc tggtcgagca actggtcgcc gcccatcacc gcgcacagcg cggtggtgca
     1081 gacgccgacg tggttgcggc cgccgggctg acgccggtac atggtgtaga aggtggccac
     1141 accgctgacc tgcgcggtgg tgatgccgag aatgtcggcg cacgcctgca ccccggccgg
     1201 gctgacccgg ccgtcgacgc tctgcacgag atgcagcatc ggcagcaggg ccgaacgggc
     1261 ctgcgggtag cgggccgcga tggagcgcat ctcgtcgagg gtggtctcgg tgatgttcgt
     1321 cgaggtgtcg tcgtagtcga tgtgccccga ctcggggaaa tatgactgga actcgtggcc
     1381 gctcatcggt ccacccctcc catcaccggg tcgagcgagc cgaccgccac caccacgtcc
     1441 gagatcattc cgccttcgca ctggatcggc accgactgca ggtggttgaa cccggggtcg
     1501 cgcatgtgca cccggaacgg cctcgtgccg ccgtcgctga ccacgtggca ggcgatctcg
     1561 cccttgggcg actcgatcgg cacgtaggcc tggccgggcg gcacccggaa gccctcggtg
     1621 acgatcttga agtggtggat cagcgcctcc atggattcac ccatgatgtg cttgatgtgc
     1681 tcgtggctgt tgccctggcc gtcgggaccg agcgccagct gtgccggcca ggcgatcttg
     1741 gggtcggcca ccatcaccgg ctcgccgcgg ctggcctcca gccggtcggc acattgctcg
     1801 acgatgcgca ggctctgcca catctcttcg agccggatgc ggaaccgcgc gtagctgtcg
     1861 gacgaggccc agccgtcctc gggccgccag gccaccgagt cgaactcgta ggtttcgtag
     1921 ccgcagtagg gctgctgctt gcgcaggtcc cagtcgtagc cggtggagcg cagcaccggg
     1981 ccggtgacgc cgagagccat gcaggcgctc aggtcttgat ggccgatgcc gatcatgcgc
     2041 atcttgaaga tcgggttctc gttgcagaac gccgcgtact cgggcaggtg cttgtgcagc
     2101 cagtcgatca gctcacgcaa ctgcgcgatg ccggtttcgg gcaggtcgtt cgccaccccg
     2161 ccgggacgga tgaaggcgtg gttcatacgc agcccggtga ccgcctcgaa gaagtcgagg
     2221 atcatctcgc gctcgcggaa gccgatcgtc atcaccgtga gtgcaccgat ctccataccg
     2281 ccggtgccga tggcgaccag gtgggagccg atgcggttga gttccatcat cagcacccgg
     2341 atcacggtgg cccgctcggg gatgtcggct tcgatgccca gcagccgctc gacggccagg
     2401 cagtaggccg cctcgttgaa gatcggcgcc acgtagtcca tgcgggtgac gaaggtggag
     2461 ccctgcgtcc aggtgcggta ctccatgttc ttctcgatac cggtgtgcag aaagccgatg
     2521 cctggacgaa tgctcagcac cgtttcgccg tcgcactcaa ggatcagtcg cagcacgccg
     2581 tgcgtggacg ggtgctgcgg gcccatgttg atcaccagga tctcgtcgga gcgttcggcc
     2641 tgctcggcgg cgatctcggc ccagtcaccg cccatcgcgg tgtagacggt gccctcggtc
     2701 tcttcgccgg gctgggcgta gaagtcggtg gtgctcatcg gtagctcctc cgctgatcgg
     2761 gcgg
//
LOCUS       DAOSUG010000432         2760 bp    DNA     linear   ENV 10-JUN-2024
DEFINITION  MAG TPA_asm: Micropruina sp. isolate AMR_MDS_2195
            NODE_14805_length_2760_cov_3.174492, whole genome shotgun sequence.
ACCESSION   DAOSUG010000432 DAOSUG010000000
VERSION     DAOSUG010000432.1
DBLINK      BioProject: PRJNA925477
            BioSample: SAMN32864262
            Sequence Read Archive: SRR4236673
KEYWORDS    WGS; ENV; Metagenome Assembled Genome; MAG; Third Party Data; TPA;
            TPA:assembly.
SOURCE      Micropruina sp. (wastewater metagenome)
  ORGANISM  Micropruina sp.
            Bacteria; Bacillati; Actinomycetota; Actinomycetes;
            Propionibacteriales; Nocardioidaceae; Micropruina.
REFERENCE   1  (bases 1 to 2760)
  AUTHORS   Abdulkadir,N., Saraiva,J.P., Zhang,J., Stolte,S., Gillor,O.,
            Harms,H. and Rocha,U.
  TITLE     Genome-centric analyses of 165 metagenomes show that mobile genetic
            elements are crucial for the transmission of antimicrobial
            resistance genes to pathogens in activated sludge and wastewater
  JOURNAL   Microbiol Spectr 12 (3), e0291823 (2024)
   PUBMED   38289113
REFERENCE   2  (bases 1 to 2760)
  AUTHORS   Abdulkadir,N.
  TITLE     Direct Submission
  JOURNAL   Submitted (24-MAR-2023) Environmental Microbiology, Helmholtz
            Center for Environmental Research -UFZ, Permoserstr 15, Leipzig
            04318, Germany
COMMENT     The annotation was added by the NCBI Prokaryotic Genome Annotation
            Pipeline (PGAP). Information about PGAP can be found here:
            https://www.ncbi.nlm.nih.gov/genome/annotation_prok/
            
            ##Genome-Assembly-Data-START##
            Assembly Method        :: SPAdes v. 3.11.1
            Genome Representation  :: Full
            Expected Final Version :: Yes
            Genome Coverage        :: Not Applicable
            Sequencing Technology  :: Illumina MiSeq
            ##Genome-Assembly-Data-END##
            
            ##Genome-Annotation-Data-START##
            Annotation Provider               :: NCBI
            Annotation Date                   :: 05/04/2023 04:57:10
            Annotation Pipeline               :: NCBI Prokaryotic Genome
                                                 Annotation Pipeline (PGAP)
            Annotation Method                 :: Best-placed reference protein
                                                 set; GeneMarkS-2+
            Annotation Software revision      :: 6.5
            Features Annotated                :: Gene; CDS; rRNA; tRNA; ncRNA
            Genes (total)                     :: 2,776
            CDSs (total)                      :: 2,750
            Genes (coding)                    :: 2,714
            CDSs (with protein)               :: 2,714
            Genes (RNA)                       :: 26
            tRNAs                             :: 24
            ncRNAs                            :: 2
            Pseudo Genes (total)              :: 36
            CDSs (without protein)            :: 36
            Pseudo Genes (ambiguous residues) :: 0 of 36
            Pseudo Genes (frameshifted)       :: 2 of 36
            Pseudo Genes (incomplete)         :: 32 of 36
            Pseudo Genes (internal stop)      :: 2 of 36
            ##Genome-Annotation-Data-END##
FEATURES             Location/Qualifiers
     source          1..2760
                     /organism="Micropruina sp."
                     /mol_type="genomic DNA"
                     /submitter_seqid="NODE_14805_length_2760_cov_3.174492"
                     /isolate="AMR_MDS_2195"
                     /isolation_source="Activated sludge of municipal
                     wastewater treatment plants"
                     /db_xref="taxon:2737536"
                     /environmental_sample
                     /geo_loc_name="Canada"
                     /lat_lon="49.25 N 123.1 W"
                     /collection_date="2015-06-16"
                     /metagenome_source="wastewater metagenome"
                     /note="metagenomic"
     gene            complement(45..941)
                     /locus_tag="PLF56_12290"
     CDS             complement(45..941)
                     /locus_tag="PLF56_12290"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_011754767.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_process: GO:0008643 - carbohydrate transport [Evidence
                     IEA];
                     GO_process: GO:0055085 - transmembrane transport [Evidence
                     IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="carbohydrate ABC transporter permease"
                     /protein_id="HOQ54377.1"
                     /translation="MSTTLELPLDAGDVAPAPRRKRGVTPMRAVQFILLVLFASAVLI
                     PLYVVLVTSFKGGVDVSPSSAWALPSVFSTDGWTTAITRLAPALGRSLILALSAAVLS
                     SILGSLNGYVFAKWRFPGADIVFTAFLFGMFIPYQAVMIPLQKMLLDLQNHGFGFLDG
                     IPKLILVHTIYGIPICTLIFRNYYATAVPNEILEAARVDGAGMLRTYWSIVLPVSIPG
                     FVVTLIWQFTSAWNDFLFALFLTSPRNGPVTYALQELAGGQNPDYPSIMAGVLVASLP
                     TLIVYIALGRYFISGLMSGSVK"
     gene            complement(941..1846)
                     /locus_tag="PLF56_12295"
     CDS             complement(941..1846)
                     /locus_tag="PLF56_12295"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_002520120.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_function: GO:0042626 - ATPase-coupled transmembrane
                     transporter activity [Evidence IEA];
                     GO_function: GO:0140359 - ABC-type transporter activity
                     [Evidence IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="sugar ABC transporter permease"
                     /protein_id="HOQ54378.1"
                     /translation="MRRLRTWGPGLLLVSPSIILVGIFVYGLIGVNVSTSLAQTRDRI
                     TNKITNPGGLQNYSNLLADDRYQHALWNLLVLTVVFMTGTMVFGLLWAVLLERGVTGE
                     GVFRSIYLFPMAISFVAAGVAWRWLLSPAQGADQAIGLNQLFLSLGMPWLQSKWWSDP
                     GPFNMAAMAIPAIWQLSGYIMALFLAGFRGISDDQREAARVDGASEWQLYRYVLFPQV
                     SPVALSALIILGHMSMKMFDLIYSIAGANSYTAEVPATFMWIQLFPQSNPTGAAANAT
                     IILAIVAVVVVPYLIYTNRTEKGGR"
     gene            complement(1914..>2760)
                     /locus_tag="PLF56_12300"
     CDS             complement(1914..>2760)
                     /locus_tag="PLF56_12300"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_002515410.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_component: GO:0055052 - ATP-binding cassette (ABC)
                     transporter complex, substrate-binding subunit-containing
                     [Evidence IEA];
                     GO_function: GO:0042626 - ATPase-coupled transmembrane
                     transporter activity [Evidence IEA];
                     GO_function: GO:0140359 - ABC-type transporter activity
                     [Evidence IEA]"
                     /codon_start=2
                     /transl_table=11
                     /product="ABC transporter substrate-binding protein"
                     /protein_id="HOQ54379.1"
                     /translation="GSIYSVPSNIHRANVVWTNPEVLKKANITAEPADIDAWLADMAK
                     LKAAGVSTPLTIGGTWTQVQLFETVLIADLGVDGYVALFKDGADWGSDGVKKAVEHYS
                     KLLGYANTASDGDDWPGAIDAVIDGKAAYNVMGDWAVAEFTAKGKKDGTDYTYFPVPG
                     TKGVFDFLADSFTLPKGAKNPGGAKNWLELVGSAEGQKAFNLAKGSIPARTDVPASDF
                     PPYQQSAMEAFKTDKIAPSIAHGAAVSQAWNSDISSAISKFYGSKDQATLVTDLVAAA
                     KKNLA"
BASE COUNT          498 a          821 c          980 g          461 t
ORIGIN      
        1 gcttgtcatc ccggcggacg ccgggatctc gcgcagcgcg tgggctactt cactgacccg
       61 ctcatcaggc cgctgatgaa gtaccggccg agtgcgatgt agacgatcag ggtgggcagc
      121 gacgcgacga gcacaccggc catgatgctg gggtagtcgg ggttctgccc gccggcgagt
      181 tcctgcaggg cgtaggtgac ggggccgttg cgcggcgagg tgaggaacaa cgcgaacagg
      241 aagtcgttcc aggcgctggt gaactgccag atcagcgtga ccacgaagcc gggaatgctg
      301 accggcagca caatggacca gtaggtgcgc agcatgccgg cgccgtccac ccgggcggct
      361 tcgaggattt cgttgggtac ggccgtggcg tagtagttgc gaaagatcag ggtgcagatc
      421 gggatgccgt agatggtgtg caccaggatc agtttgggga tgccgtccag aaagccgaac
      481 ccgtggttct gcaggtcgag cagcattttc tgtagcggaa tcatcactgc ctgataaggg
      541 atgaacatgc cgaacagaaa tgcggtgaac acgatgtcgg cgccgggaaa cctccacttg
      601 gcgaacacgt agccgttgag cgagccgagt atggacgaca acacggccgc cgacagggca
      661 agaatcagtg agcggcccag agcaggtgcg aggcgggtga tggcggtcgt ccagccgtcg
      721 gtggagaaca cgctgggcaa cgcccaagcc gacgacggcg agacgtccac gccgcccttg
      781 aagctggtca ccagcaccac gtagaggggg atcagcacgg cgctggcgaa cagcaccagc
      841 agtatgaact ggacggcgcg catcggggtc acgccgcgct tgcggcgcgg tgccggggcg
      901 acgtcgccgg catccagtgg cagttcgagg gtggtgctca tcagcggccg cccttctcgg
      961 tgcggttggt gtagatcagg tagggcacca ccacgacggc cacgatggcc aaaatgatgg
     1021 tggcgtttgc ggccgcgccg gtggggttgg attgcggaaa gagctggatc cacatgaagg
     1081 tggcaggcac ctcggcggtg tagctgttgg cgccggcgat ggagtagatc aggtcgaaca
     1141 tcttcatcga catgtgcccg agaatgatca gcgccgacag ggcgacgggc gagacctgag
     1201 gaaagagtac gtaacggtac agctgccact cggacgcgcc gtcgacccgg gcggcctcgc
     1261 gctggtcgtc gctgatgccg cggaaaccgg ccaggaacag cgccatgatg tagccgctga
     1321 gctgccagat cgccggaatg gccatggccg ccatgttgaa cgggcccggg tcggaccacc
     1381 atttgctctg cagccagggc atgccgaggc tgaggaacag ctggttgagg ccgatcgcct
     1441 ggtcggcgcc ctgggcgggg gacaggagcc agcgccaggc gacgccggcg gcgacgaacg
     1501 agatcgccat cggaaacagg tagatggagc ggaagacgcc ctcaccggtg acgccgcgct
     1561 cgagcagcac cgcccacagc aggccgaaca ccatggtgcc ggtcatgaat accacggtca
     1621 gcaccagcag gttccacagg gcgtgctggt agcggtcgtc ggccagcagg ttggagtagt
     1681 tctgcagtcc gccggggttg gtgatcttgt tggtgatccg gtccctggtc tgggccagcg
     1741 acgtcgagac gttcaccccg atcaggccgt agacgaagat gccgaccaga atgatggacg
     1801 ggctgaccag gagcaggccc ggcccccagg ttcgtagccg gcgcatgcgg cctcctcacg
     1861 tgcgtgcggg ggtgcgtccg aagggatcgg acgcaccccc ggtggcggtt cgctcaggcc
     1921 aggttcttct tggctgcggc gaccaggtcg gtgaccaggg tggcctggtc cttgctgccg
     1981 tagaacttcg agatcgcgga gctgatgtca gagttccagg cttggctgac tgccgcaccg
     2041 tgggcgatgg acggcgcgat cttgtcggtc ttgaacgcct ccatcgcgga ctgctggtac
     2101 ggcgggaagt cactggctgg cacgtcggtg cgggccggga tcgagccctt tgccaggttg
     2161 aacgcctttt ggccctcggc cgaaccgacc agttcgagcc agttcttcgc accgccgggg
     2221 ttcttggcgc ccttgggcag ggtgaacgag tcagcaagga agtcgaacac acccttggtg
     2281 ccgggcaccg ggaagtaggt gtagtcggtg ccgtccttct tgcccttggc ggtgaactcg
     2341 gcgactgccc agtcacccat cacgttgtag gcggccttgc cgtcgatgac cgcgtcgatg
     2401 gcgcccggcc agtcgtcgcc gtcggaggcg gtgttggcgt agccgagcag cttggagtag
     2461 tgctcgacgg ccttcttgac cccgtcggaa ccccagtcgg caccgtcctt gaacaaggcg
     2521 acatagccgt cgacacccag atcggcgatc agcacggtct cgaacagctg cacctgggtc
     2581 caggtgccgc cgatcgtcag cggcgtcgac acgcctgccg ccttcagctt ggccatgtcg
     2641 gccagccagg cgtcgatgtc cgccggctca gcggtgatgt tggccttctt caacacctcg
     2701 gggttcgtcc acaccacgtt cgcgcggtga atgtttgacg gcaccgagta gatgctgccc
//
LOCUS       DAOSUG010000433         2755 bp    DNA     linear   ENV 10-JUN-2024
DEFINITION  MAG TPA_asm: Micropruina sp. isolate AMR_MDS_2195
            NODE_14843_length_2755_cov_3.247778, whole genome shotgun sequence.
ACCESSION   DAOSUG010000433 DAOSUG010000000
VERSION     DAOSUG010000433.1
DBLINK      BioProject: PRJNA925477
            BioSample: SAMN32864262
            Sequence Read Archive: SRR4236673
KEYWORDS    WGS; ENV; Metagenome Assembled Genome; MAG; Third Party Data; TPA;
            TPA:assembly.
SOURCE      Micropruina sp. (wastewater metagenome)
  ORGANISM  Micropruina sp.
            Bacteria; Bacillati; Actinomycetota; Actinomycetes;
            Propionibacteriales; Nocardioidaceae; Micropruina.
REFERENCE   1  (bases 1 to 2755)
  AUTHORS   Abdulkadir,N., Saraiva,J.P., Zhang,J., Stolte,S., Gillor,O.,
            Harms,H. and Rocha,U.
  TITLE     Genome-centric analyses of 165 metagenomes show that mobile genetic
            elements are crucial for the transmission of antimicrobial
            resistance genes to pathogens in activated sludge and wastewater
  JOURNAL   Microbiol Spectr 12 (3), e0291823 (2024)
   PUBMED   38289113
REFERENCE   2  (bases 1 to 2755)
  AUTHORS   Abdulkadir,N.
  TITLE     Direct Submission
  JOURNAL   Submitted (24-MAR-2023) Environmental Microbiology, Helmholtz
            Center for Environmental Research -UFZ, Permoserstr 15, Leipzig
            04318, Germany
COMMENT     The annotation was added by the NCBI Prokaryotic Genome Annotation
            Pipeline (PGAP). Information about PGAP can be found here:
            https://www.ncbi.nlm.nih.gov/genome/annotation_prok/
            
            ##Genome-Assembly-Data-START##
            Assembly Method        :: SPAdes v. 3.11.1
            Genome Representation  :: Full
            Expected Final Version :: Yes
            Genome Coverage        :: Not Applicable
            Sequencing Technology  :: Illumina MiSeq
            ##Genome-Assembly-Data-END##
            
            ##Genome-Annotation-Data-START##
            Annotation Provider               :: NCBI
            Annotation Date                   :: 05/04/2023 04:57:10
            Annotation Pipeline               :: NCBI Prokaryotic Genome
                                                 Annotation Pipeline (PGAP)
            Annotation Method                 :: Best-placed reference protein
                                                 set; GeneMarkS-2+
            Annotation Software revision      :: 6.5
            Features Annotated                :: Gene; CDS; rRNA; tRNA; ncRNA
            Genes (total)                     :: 2,776
            CDSs (total)                      :: 2,750
            Genes (coding)                    :: 2,714
            CDSs (with protein)               :: 2,714
            Genes (RNA)                       :: 26
            tRNAs                             :: 24
            ncRNAs                            :: 2
            Pseudo Genes (total)              :: 36
            CDSs (without protein)            :: 36
            Pseudo Genes (ambiguous residues) :: 0 of 36
            Pseudo Genes (frameshifted)       :: 2 of 36
            Pseudo Genes (incomplete)         :: 32 of 36
            Pseudo Genes (internal stop)      :: 2 of 36
            ##Genome-Annotation-Data-END##
FEATURES             Location/Qualifiers
     source          1..2755
                     /organism="Micropruina sp."
                     /mol_type="genomic DNA"
                     /submitter_seqid="NODE_14843_length_2755_cov_3.247778"
                     /isolate="AMR_MDS_2195"
                     /isolation_source="Activated sludge of municipal
                     wastewater treatment plants"
                     /db_xref="taxon:2737536"
                     /environmental_sample
                     /geo_loc_name="Canada"
                     /lat_lon="49.25 N 123.1 W"
                     /collection_date="2015-06-16"
                     /metagenome_source="wastewater metagenome"
                     /note="metagenomic"
     gene            <1..263
                     /locus_tag="PLF56_12305"
     CDS             <1..263
                     /locus_tag="PLF56_12305"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_013161000.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=3
                     /transl_table=11
                     /product="methionine biosynthesis protein MetW"
                     /protein_id="HOQ54380.1"
                     /translation="PNFGLLQHRLMLLRGRMPMSRELPYSWYDTPNLRFTTLVDLEDL
                     FAKLGLVIVQRINLSLTGEPLGKGFRAANLRAGAAVYELRAR"
     gene            complement(349..816)
                     /locus_tag="PLF56_12310"
     CDS             complement(349..816)
                     /locus_tag="PLF56_12310"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_020390153.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_function: GO:0003676 - nucleic acid binding [Evidence
                     IEA];
                     GO_function: GO:0004519 - endonuclease activity [Evidence
                     IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="HNH endonuclease"
                     /protein_id="HOQ54381.1"
                     /translation="MAVLILNTDDTALHTVSLQHAIGMLVRKVAVVEAAHPDLVFGSA
                     PQPEVLRLVRYVKTTFLYARVPGWTKRGVLKRDRHTCGYCGERASTVDHIVPISRGGR
                     NTWTNTVAACHSCNARKANHTPSEAGMPLRLKPATPTRSQLMVPERAGEERAA"
     gene            complement(1104..2018)
                     /locus_tag="PLF56_12315"
     CDS             complement(1104..2018)
                     /locus_tag="PLF56_12315"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_013863446.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="5'-3' exonuclease"
                     /protein_id="HOQ54382.1"
                     /translation="MDRPNLQLFDTASLYFRAFYGLPSSLRGQDGRSTNAVRGLLDFM
                     ARFITDYRPTHAAACWDNSWRPAWRVDLVPSYKTHRVLSGSTEVVPEDLTPQVPLILA
                     VLRALGIAVVGADEFEADDVIGTLATHSAVPVTVVTGDRDLFQLVDDARGVRVLSVVK
                     GVRNHEVIDEAALVAKYGVGGAGYADFATLRGDASDGLPGVAGVGEKTAARLIADYGD
                     LEGVLTAASERRLAPGLTAKLTAAIDYLAAAREVVRVRTDVPLDVSLDDLVLPTAPAQ
                     PEVFTELADLLNLGGAADRIVAALALNA"
     gene            2044..2481
                     /locus_tag="PLF56_12320"
     CDS             2044..2481
                     /locus_tag="PLF56_12320"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_013863445.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_function: GO:0003677 - DNA binding [Evidence IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="Lrp/AsnC family transcriptional regulator"
                     /protein_id="HOQ54383.1"
                     /translation="MESTDRQILTLLAKDGRMSYTDIGRETGLSTSAAQQRVRRLEQR
                     GLITGYRAELSADALGLVLTAFIELKGSDPATDDQIPERLAHLTNIVSCYSVAGDASY
                     ILIVQVETPGDLETLLAHIRTAAGVSTLTTLVLSVPFKDRPVL"
     gene            2530..>2755
                     /locus_tag="PLF56_12325"
     CDS             2530..>2755
                     /locus_tag="PLF56_12325"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_015070408.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="hypothetical protein"
                     /protein_id="HOQ54384.1"
                     /translation="MTDRLRALPWWVRTSLATVAGAAASLGFAPVGWWPVLIVCVAAL
                     TLLAITAPRWWHAALVGLGYGFGLYLIAIRW"
BASE COUNT          415 a          958 c          960 g          422 t
ORIGIN      
        1 tgcccaactt cggcctgctg cagcaccggc tgatgctgct acggggccgc atgccgatgt
       61 cgcgcgagtt gccgtacagc tggtacgaca cgccgaacct gcggttcacc accctggtcg
      121 atctggaaga cttgttcgcc aagctcggcc tggtgatcgt gcagcgcatc aacctgtcgc
      181 tcaccggcga gccgctgggc aagggcttcc gggcagccaa ccttcgcgcc ggcgcggcgg
      241 tctacgaact gcgggcacgc tgacggggac gctgggcttt gggttcgtcg gttgcgctcg
      301 tcgagacctc gtcgtccgca gtggtagacc caacgagtcc gggtcccgtc aggctgcgcg
      361 ctcctcgccg gcccgttccg gcaccatcag ttgggagcgc gtgggggtcg ccggcttgag
      421 ccgcagcggc atgccggcct cggacggggt gtggttggcc ttgcgcgcgt tgcaggagtg
      481 gcaggccgcc acggtgttgg tccaggtgtt gcgaccgccc cggctgatcg gcacgatgtg
      541 gtcgacggtg gacgcccgct cgccgcagta cccgcaggtg tgccggtcgc gcttgagcac
      601 gccccgcttc gtccagccgg gtacccgggc gtagagaaac gtcgtcttca cgtagcgaac
      661 cagccgcagc acctcgggtt ggggcgcaga accgaacacc aagtcggggt gcgcggcctc
      721 gaccacagcc accttgcgca ccagcatgcc gatggcgtgc tgcagcgaca ctgtgtgcag
      781 cgcggtgtcg tcggtgttga gaatcagcac ggccatgcgg gtgccccttc aacggatgcg
      841 gtgtggcgaa tcgggtccgg gcagaaaaaa accgccccgg gtgagcgcgg gacggtgcca
      901 tcacaggccg gaggcagtgg ggtgacctgc agtatctgca cgctgggcag tggacgctcg
      961 gcgcgggtgc gtaggagtgc actcatcgaa acccctcgag tatcgaagac gagcacactg
     1021 taacgcccac cgcgccgcaa cgggagacga ctcggcgggc ggtccataca ggcgccgccg
     1081 gccggccgga cgctgcgccc cgctcaggcg ttgagtgcga gggccgcgac gatgcggtcc
     1141 gccgcaccgc cgaggttcag caggtcggcc agctcggtga acacctcggg ttgcgccggt
     1201 gcggtcggga gcacgagatc gtccagcgac acgtccagcg gtacatcggt gcgcacccgc
     1261 accacctcgc gcgccgcggc caggtagtcg atggctgcgg tcagcttcgc ggtcagtccg
     1321 ggggccagcc gccgctcgga cgccgcggtg agcacgccct ccaggtcgcc gtagtcggcg
     1381 atcaggcggg ccgcggtctt ctccccgacc ccggcgacgc ccggcagccc gtccgacgcg
     1441 tcgccgcgca gcgtggcgaa atcggcgtag ccggcgccgc ccacgccgta cttggcgacc
     1501 agtgccgcct cgtcgatcac ctcgtggttg cgcacgccct tgaccaccga cagcacccgg
     1561 acgccccgcg catcgtccac caactgaaac aggtcgcgat ccccggtcac caccgtcacc
     1621 ggcacggcgg agtgggtggc cagcgtgccg atcacgtcgt cggcctcgaa ctcgtccgca
     1681 ccgaccacag cgatacccag cgcccgcagc acggcaagga tcagcggcac ctggggggtg
     1741 aggtcctcgg gcaccacctc ggtgctgccg ctcagcaccc ggtgtgtctt gtagctaggc
     1801 accagatcga cccgccaggc gggccgccac gagttgtccc agcaggcggc ggcgtgggtc
     1861 gggcggtagt cggtgatgaa cctggccatg aagtccagca ggccgcggac ggcgttggtg
     1921 cttcgtccgt cctgaccgcg cagcgacgac ggcagaccgt agaaggcccg gaaatacagc
     1981 gatgcggtgt cgaaaagttg cagattcggc cggtccatcc gcacatggtg acacgatgac
     2041 gacatggaga gcaccgaccg ccagatcctc accctgctgg ccaaggacgg gcgcatgtcg
     2101 tacaccgaca tcggccgaga gaccggcctg tcgacctcgg cggcccagca acgggtgcga
     2161 cgtttggagc agcgcggcct gatcaccggc taccgggccg agttgagcgc ggacgcgctg
     2221 ggcctggtgc tgaccgcctt catcgagttg aaggggtccg acccggccac cgacgaccag
     2281 atccccgagc ggctcgcgca cctgaccaac atcgtctcgt gctactcggt ggccggtgac
     2341 gccagctaca tactgatcgt gcaggtcgag acccccggcg acctcgagac gctgctggcc
     2401 cacatcagga ctgccgccgg cgtctcgacc ctgaccaccc tggtgctgtc ggtgccgttc
     2461 aaggaccgcc ctgtgctctg agtgaggccg agcagatagt cacagccggc actgggtacg
     2521 ctgcgcagcg tgactgaccg cctgcgcgcc ctgccctggt gggtgcgcac ttcgctggcc
     2581 acggtggccg gcgccgcggc gtccctcggc ttcgccccgg tgggttggtg gccggtgctc
     2641 atcgtctgcg tcgccgcact gacgctgctc gcgatcacgg ccccgcgctg gtggcacgcg
     2701 gccctggtgg ggctgggtta cggcttcggc ctgtacctga tcgccattcg ctgga
//
LOCUS       DAOSUG010000434         2754 bp    DNA     linear   ENV 10-JUN-2024
DEFINITION  MAG TPA_asm: Micropruina sp. isolate AMR_MDS_2195
            NODE_14851_length_2754_cov_3.821415, whole genome shotgun sequence.
ACCESSION   DAOSUG010000434 DAOSUG010000000
VERSION     DAOSUG010000434.1
DBLINK      BioProject: PRJNA925477
            BioSample: SAMN32864262
            Sequence Read Archive: SRR4236673
KEYWORDS    WGS; ENV; Metagenome Assembled Genome; MAG; Third Party Data; TPA;
            TPA:assembly.
SOURCE      Micropruina sp. (wastewater metagenome)
  ORGANISM  Micropruina sp.
            Bacteria; Bacillati; Actinomycetota; Actinomycetes;
            Propionibacteriales; Nocardioidaceae; Micropruina.
REFERENCE   1  (bases 1 to 2754)
  AUTHORS   Abdulkadir,N., Saraiva,J.P., Zhang,J., Stolte,S., Gillor,O.,
            Harms,H. and Rocha,U.
  TITLE     Genome-centric analyses of 165 metagenomes show that mobile genetic
            elements are crucial for the transmission of antimicrobial
            resistance genes to pathogens in activated sludge and wastewater
  JOURNAL   Microbiol Spectr 12 (3), e0291823 (2024)
   PUBMED   38289113
REFERENCE   2  (bases 1 to 2754)
  AUTHORS   Abdulkadir,N.
  TITLE     Direct Submission
  JOURNAL   Submitted (24-MAR-2023) Environmental Microbiology, Helmholtz
            Center for Environmental Research -UFZ, Permoserstr 15, Leipzig
            04318, Germany
COMMENT     The annotation was added by the NCBI Prokaryotic Genome Annotation
            Pipeline (PGAP). Information about PGAP can be found here:
            https://www.ncbi.nlm.nih.gov/genome/annotation_prok/
            
            ##Genome-Assembly-Data-START##
            Assembly Method        :: SPAdes v. 3.11.1
            Genome Representation  :: Full
            Expected Final Version :: Yes
            Genome Coverage        :: Not Applicable
            Sequencing Technology  :: Illumina MiSeq
            ##Genome-Assembly-Data-END##
            
            ##Genome-Annotation-Data-START##
            Annotation Provider               :: NCBI
            Annotation Date                   :: 05/04/2023 04:57:10
            Annotation Pipeline               :: NCBI Prokaryotic Genome
                                                 Annotation Pipeline (PGAP)
            Annotation Method                 :: Best-placed reference protein
                                                 set; GeneMarkS-2+
            Annotation Software revision      :: 6.5
            Features Annotated                :: Gene; CDS; rRNA; tRNA; ncRNA
            Genes (total)                     :: 2,776
            CDSs (total)                      :: 2,750
            Genes (coding)                    :: 2,714
            CDSs (with protein)               :: 2,714
            Genes (RNA)                       :: 26
            tRNAs                             :: 24
            ncRNAs                            :: 2
            Pseudo Genes (total)              :: 36
            CDSs (without protein)            :: 36
            Pseudo Genes (ambiguous residues) :: 0 of 36
            Pseudo Genes (frameshifted)       :: 2 of 36
            Pseudo Genes (incomplete)         :: 32 of 36
            Pseudo Genes (internal stop)      :: 2 of 36
            ##Genome-Annotation-Data-END##
FEATURES             Location/Qualifiers
     source          1..2754
                     /organism="Micropruina sp."
                     /mol_type="genomic DNA"
                     /submitter_seqid="NODE_14851_length_2754_cov_3.821415"
                     /isolate="AMR_MDS_2195"
                     /isolation_source="Activated sludge of municipal
                     wastewater treatment plants"
                     /db_xref="taxon:2737536"
                     /environmental_sample
                     /geo_loc_name="Canada"
                     /lat_lon="49.25 N 123.1 W"
                     /collection_date="2015-06-16"
                     /metagenome_source="wastewater metagenome"
                     /note="metagenomic"
     gene            complement(<1..1866)
                     /gene="uvrC"
                     /locus_tag="PLF56_12330"
     CDS             complement(<1..1866)
                     /gene="uvrC"
                     /locus_tag="PLF56_12330"
                     /EC_number="3.1.25.-"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_002513875.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_component: GO:0009380 - excinuclease repair complex
                     [Evidence IEA];
                     GO_function: GO:0003677 - DNA binding [Evidence IEA];
                     GO_function: GO:0005515 - protein binding [Evidence IEA];
                     GO_function: GO:0009381 - excinuclease ABC activity
                     [Evidence IEA];
                     GO_process: GO:0006289 - nucleotide-excision repair
                     [Evidence IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="excinuclease ABC subunit UvrC"
                     /protein_id="HOQ54385.1"
                     /translation="MADPESYRPATGTIPTQPGVYRFSDAAGRVIYVGKAINLRQRLT
                     SYFADPALLHFRTRTMVRTAAGVEWTVVRNELEALQLEYTWIKQHDPRFNVKYRDDKS
                     YPWVAVTWSEEYPTVFVGRGKKRKGIRYFGPYAHAWAIRETLDPLLRAFPMRSCTNGV
                     FRAAAASGRPCLLGYIGKCAAPCVGRIGPDEHRALAQDVCDFLAGRTNTLLRRLERQM
                     SEASANLEFEKAAVLRDQLHALRQATDSNAIVLGDGTDADVIALAEDPLEVAVQIFHV
                     RDGRVRGQRAWVADRTDDTETGGLIETFLLQLYGDVDGTEVPPEILVPQVPSTGGLIA
                     ALLTESRGARVVIRTPRRGDKKTLMETVARNAAEALALHKTRRASDLSTRNRALEEIQ
                     RALELPTAPLRIECYDVSHLQGTEIVASMVVFEDGLARKSDYRRFVIRGIDGSNDVAA
                     IHEVITRRFRRLLDEQGGTDDEAGPLLVDPTTGAARKFAYAPGLVVVDGGAPQVAAAA
                     QALGELGITDIPVIGLAKRLEEVWLPGEEFPLILPRTSEGLYLLQRVRDEAHRFAITH
                     HRGRRSASMVESVLDAVPGLGEVRRKTLLKHFGSLRKLRAATPEELSVVPGIGPRL"
     gene            complement(1866..>2754)
                     /locus_tag="PLF56_12335"
     CDS             complement(1866..>2754)
                     /locus_tag="PLF56_12335"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_013862268.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=2
                     /transl_table=11
                     /product="excinuclease ABC subunit A"
                     /protein_id="HOQ54386.1"
                     /translation="HKGLIGAGFIDKVIHVDQSPIGRTPRSNPATYTGVFDKIRTLFA
                     ATPEAKMRGYQPGRFSFNVKGGRCDNCTGDGTIKIEMNFLPDVYVPCEVCHGARYNRE
                     TLEVHFKGRTIAEVLDMPIEEAVEFFSAIPAITRHLRTLVDVGLGYVRLGQPATTLSG
                     GEAQRVKLASELQRRSTGRTMYVLDEPTTGLHFEDIRKLLGVLGRLVDAGNTVVVIEH
                     NLDVIKTADWVIDMGPDGGSRGGQVIAEGTPEQIAATPASYTGEFLRPVLHGHQVPVA
                     AAPEPLIEAPPPQRRRRAS"
BASE COUNT          418 a          906 c          982 g          448 t
ORIGIN      
        1 gaggcggggg ccgatgcccg gaaccaccga cagttcctcg ggtgtcgccg cgcgcagctt
       61 ccgcagtgaa ccgaagtgct tcagcagcgt cttgcgccgc acctcaccca ggccggggac
      121 ggcgtccagc accgactcga ccatggacgc gctgcgccgt ccgcgatggt gggtgatggc
      181 gaaacggtgc gcctcatcgc ggacgcgctg cagcaggtag aggccctcgc tggtgcgcgg
      241 caggatcagc gggaactcct cccccggcag ccacacttct tcgagccgct tggccagccc
      301 gatcacggga atgtcggtga tacccagctc gcccaatgcc tgggcggccg cggcgacctg
      361 gggcgcgccc ccgtccacca cgaccaggcc gggggcatag gcgaacttgc gggcggcgcc
      421 ggtggtcggg tcgaccagca gcggacctgc ctcgtcgtcg gtaccgccct gctcgtcgag
      481 caggcgccgg aaccggcggg tgatcacttc gtggatggcc gcgacgtcgt tggagccatc
      541 gatgccgcgg atcacaaagc gccggtagtc gctcttgcgg gcgaggccgt cttcgaacac
      601 caccatggac gcgacgatct cggtgccctg caggtgcgat acgtcgtaac actcgatgcg
      661 cagcggtgcg gtgggcagtt cgagcgcccg ctggatctct tcgagggccc ggttgcgggt
      721 ggagagatcg ctggcacgac gcgtcttgtg cagggccagc gcctcggcgg cgttacgggc
      781 cacggtttcc atcaacgtct tcttgtcgcc gcgacgcgga gtgcggatca ccacccgggc
      841 gccacgggat tcggtgagca gtgcggcgat cagaccgccc gtggacggca cctgcggcac
      901 caggatctcg ggcggcacct cggtgccgtc gacatcgccg tagagctgca gcagaaaggt
      961 ctcgatcagc ccgccggttt cggtgtcgtc ggtgcggtcg gccacccagg cgcgctgacc
     1021 acggacgcgt ccgtcgcgca cgtggaagat ctgcaccgcg acctcgagcg ggtcttcggc
     1081 aagcgcgatc acgtcggcgt cggtgccgtc gcccagcacg atcgcgttgc tgtcggtggc
     1141 ctgtcgcagc gcgtgcaact gatcgcgcag caccgcggcc ttctcgaact cgaggttcgc
     1201 cgacgcctcg ctcatctgcc gttcgagccg cctgagcagc gtgttggtgc gaccggccag
     1261 aaagtcgcac acgtcctgag cgagggcccg gtgttcgtca gggccgatgc ggccgacgca
     1321 cggcgctgcg catttgccga tgtagccgag cagacagggg cgtccggacg ccgccgcggc
     1381 ccggaacacg ccgttggtgc aggaacgcat cggaaacgcg cggagcaacg ggtcgagggt
     1441 ttcgcggatc gcccaggcat gcgcgtaggg gccgaagtaa cggatgccct tgcgcttctt
     1501 gccgcgtccg acgaagaccg tggggtactc ctccgaccag gtcaccgcca cccacgggta
     1561 cgacttgtcg tcgcggtatt tgacgttgaa gcgcgggtcg tgctgcttga tccaggtgta
     1621 ctcgagttgc agcgcctcca gttcgttgcg caccacggtc cactcgacgc cggcagcggt
     1681 gcgcaccatg gtgcgggtgc ggaagtgcag aagagcgggg tcggcgaaat acgacgtcaa
     1741 tcgctgccgc aggttgatcg ccttgccgac atagatcacc cgcccggcgg cgtcactgaa
     1801 ccggtagacg ccgggttggg tcggaatggt gccggtggcc ggacggtagc tctcggggtc
     1861 ggccatcagc tggctcgacg ccgacgctgc ggcggcggcg cctcgatcag gggctcgggc
     1921 gcggccgcga ccggcacctg atgcccgtgc agcacgggtc gcagaaactc gccggtgtac
     1981 gacgcgggtg tggccgcgat ctgctcaggg gtaccctcgg cgatcacctg gccgccgcgc
     2041 gagccgccgt cgggacccat gtcgatcacc cagtcggcgg tcttgatcac gtcgaggttg
     2101 tgctcgatca cgaccaccgt gttgccggcg tcgaccagcc ggccgagcac cccgagaagc
     2161 ttgcggatgt cttcgaagtg caggccggtg gtgggctcgt ccagcacata catggtgcgt
     2221 ccggtggagc ggcgctgcag ttcgctggcc agtttgaccc gctgcgcctc gccaccggac
     2281 agggtggtgg cgggctggcc gagccggacg tacccaaggc cgacgtcgac cagggtgcgc
     2341 aggtgccggg tgatcgcggg aatggccgag aagaactcga ccgcctcttc gatcggcatg
     2401 tcgagcacct cggcgatggt gcggcccttg aaatgcacct cgagcgtctc gcggttgtag
     2461 cgcgcgccgt gacacacctc gcacggcacg tagacgtcgg gcaggaagtt catctcgatc
     2521 ttgatggtgc cgtcacccgt gcagttgtcg cagcgcccgc ccttgacgtt gaacgagaaa
     2581 cgccccggct ggtagccacg catcttggct tcgggagtcg cagcgaacag ggtgcggatc
     2641 ttgtcgaaga ccccggtgta ggtcgccggg ttggaccggg gggtgcgccc gatgggcgac
     2701 tggtcgacgt ggatcacctt gtcgatgaac ccggcaccga tcagcccctt gtgg
//
LOCUS       DAOSUG010000435         2735 bp    DNA     linear   ENV 10-JUN-2024
DEFINITION  MAG TPA_asm: Micropruina sp. isolate AMR_MDS_2195
            NODE_14984_length_2735_cov_3.061940, whole genome shotgun sequence.
ACCESSION   DAOSUG010000435 DAOSUG010000000
VERSION     DAOSUG010000435.1
DBLINK      BioProject: PRJNA925477
            BioSample: SAMN32864262
            Sequence Read Archive: SRR4236673
KEYWORDS    WGS; ENV; Metagenome Assembled Genome; MAG; Third Party Data; TPA;
            TPA:assembly.
SOURCE      Micropruina sp. (wastewater metagenome)
  ORGANISM  Micropruina sp.
            Bacteria; Bacillati; Actinomycetota; Actinomycetes;
            Propionibacteriales; Nocardioidaceae; Micropruina.
REFERENCE   1  (bases 1 to 2735)
  AUTHORS   Abdulkadir,N., Saraiva,J.P., Zhang,J., Stolte,S., Gillor,O.,
            Harms,H. and Rocha,U.
  TITLE     Genome-centric analyses of 165 metagenomes show that mobile genetic
            elements are crucial for the transmission of antimicrobial
            resistance genes to pathogens in activated sludge and wastewater
  JOURNAL   Microbiol Spectr 12 (3), e0291823 (2024)
   PUBMED   38289113
REFERENCE   2  (bases 1 to 2735)
  AUTHORS   Abdulkadir,N.
  TITLE     Direct Submission
  JOURNAL   Submitted (24-MAR-2023) Environmental Microbiology, Helmholtz
            Center for Environmental Research -UFZ, Permoserstr 15, Leipzig
            04318, Germany
COMMENT     The annotation was added by the NCBI Prokaryotic Genome Annotation
            Pipeline (PGAP). Information about PGAP can be found here:
            https://www.ncbi.nlm.nih.gov/genome/annotation_prok/
            
            ##Genome-Assembly-Data-START##
            Assembly Method        :: SPAdes v. 3.11.1
            Genome Representation  :: Full
            Expected Final Version :: Yes
            Genome Coverage        :: Not Applicable
            Sequencing Technology  :: Illumina MiSeq
            ##Genome-Assembly-Data-END##
            
            ##Genome-Annotation-Data-START##
            Annotation Provider               :: NCBI
            Annotation Date                   :: 05/04/2023 04:57:10
            Annotation Pipeline               :: NCBI Prokaryotic Genome
                                                 Annotation Pipeline (PGAP)
            Annotation Method                 :: Best-placed reference protein
                                                 set; GeneMarkS-2+
            Annotation Software revision      :: 6.5
            Features Annotated                :: Gene; CDS; rRNA; tRNA; ncRNA
            Genes (total)                     :: 2,776
            CDSs (total)                      :: 2,750
            Genes (coding)                    :: 2,714
            CDSs (with protein)               :: 2,714
            Genes (RNA)                       :: 26
            tRNAs                             :: 24
            ncRNAs                            :: 2
            Pseudo Genes (total)              :: 36
            CDSs (without protein)            :: 36
            Pseudo Genes (ambiguous residues) :: 0 of 36
            Pseudo Genes (frameshifted)       :: 2 of 36
            Pseudo Genes (incomplete)         :: 32 of 36
            Pseudo Genes (internal stop)      :: 2 of 36
            ##Genome-Annotation-Data-END##
FEATURES             Location/Qualifiers
     source          1..2735
                     /organism="Micropruina sp."
                     /mol_type="genomic DNA"
                     /submitter_seqid="NODE_14984_length_2735_cov_3.061940"
                     /isolate="AMR_MDS_2195"
                     /isolation_source="Activated sludge of municipal
                     wastewater treatment plants"
                     /db_xref="taxon:2737536"
                     /environmental_sample
                     /geo_loc_name="Canada"
                     /lat_lon="49.25 N 123.1 W"
                     /collection_date="2015-06-16"
                     /metagenome_source="wastewater metagenome"
                     /note="metagenomic"
     gene            <1..210
                     /locus_tag="PLF56_12340"
     CDS             <1..210
                     /locus_tag="PLF56_12340"
                     /inference="COORDINATES: protein motif:HMM:NF024989.2"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="zinc-binding dehydrogenase"
                     /protein_id="HOQ54387.1"
                     /translation="YMFTRIIHLTPDVAEQGRLLAATADLVDQGRLRTTLTTTLHGLN
                     PATLRAAHEVMDAGTSIGKIAITVE"
     gene            complement(289..1644)
                     /locus_tag="PLF56_12345"
     CDS             complement(289..1644)
                     /locus_tag="PLF56_12345"
                     /inference="COORDINATES: protein motif:HMM:NF022432.2"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="DUF2786 domain-containing protein"
                     /protein_id="HOQ54388.1"
                     /translation="MSRASQSKRAARAKDRARQRARDKQGTKASGPGPSGYWSDAEEE
                     FLASPFGTPQRHAPRRRGTAELLRDAQAGEPVALLLRAAARIAPATFFAETEALLILW
                     AAELYGNGWQPTELLRFVRLQGSKAGAELIRLTIGAERAARQGPTANDPRWVEQWQLV
                     APSKRSLSPGWVHDWAAGAPVDEAKRLIFELVALLGSAPRLDQLLAPPPGYASAQAAA
                     SGPAAGTADPMLARVRALLAKAESTEHESEAMAFTAKAQGLITKHAIDLAALQSEQPV
                     TESPQQIRLFIDPPYADAKALLLQTIAEQTRCRTVFHATLTMSSVIGYPTDLTAVEML
                     LTSLLVQAQRALAEASAAAPAGSRPRSQAFRSAFLRGFTQRIGERLTEANRLAYARDD
                     ASSFLPVLRSKGALIDDFVTSHFGDTVSRTVRGGYDHAGYAHGQLAGDAAALTAGPLN
                     A"
     gene            complement(1968..2465)
                     /locus_tag="PLF56_12350"
     CDS             complement(1968..2465)
                     /locus_tag="PLF56_12350"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_013865913.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="VTT domain-containing protein"
                     /protein_id="HOQ54389.1"
                     /translation="MDPTQWGAPFEIVVAALFCIVLMRANATYWAGRLVARGAGRTRA
                     ARWMNTEGYRRAVDRINRWGAPAVSVSFLTIGVQTVVNLAAGATRMPLTRYLPASVVG
                     SIAWAFLYGTVGFVGVEAFGVLWQHSPALAVAAVLVTLAGLVWFVLRQFRATVRADGV
                     GSDSR"
BASE COUNT          418 a          956 c          971 g          390 t
ORIGIN      
        1 tacatgttca cccgcatcat ccacctgacc ccggacgtgg ccgagcaggg cagactgctg
       61 gccgccacgg ccgacctggt cgatcagggc aggctgcgta ccacgctcac caccaccctg
      121 catgggctga accccgctac gctgcgggcc gctcacgagg tgatggacgc cggcacctcg
      181 atcggcaaga tcgcgatcac cgtcgagtag agcgctgatc gagggcgcgg gcggcgggtc
      241 gagtctggtg ccgctagcga cgggtggcca tcggtggatc cggacctatc aggcgttgag
      301 cgggccggcc gtcagggccg cggcgtcgcc ggccagctgt ccgtgtgcgt agccggcgtg
      361 gtcgtagccc ccgcgcacgg ttcgcgacac cgtgtcaccg aagtgcgacg tgacgaagtc
      421 gtcgatcagc gcacccttgg agcgcaacac cgggaggaat gacgaggcgt cgtcacgtgc
      481 gtacgccagc cggttcgcct cggtcagccg ttcaccgatg cgctgcgtga agccgcgcag
      541 aaaggcggaa cgaaaggctt gcgaccgggg gcgcgacccc gccggggcgg cggcgctggc
      601 ctcggccaac gcgcgctgcg cctggaccag cagcgaggtc aacagcatct cgaccgccgt
      661 caggtcggtc gggtagccga tgacgctcga catggtgagg gtggcgtgaa acacggtgcg
      721 acaccgggtc tgctcggcaa tggtctgcag gagaagtgcc ttggcgtcgg cgtacggcgg
      781 gtcgatgaaa agccgaatct gctgtgggct ctcggtgacc ggctgctcgc tctgcagtgc
      841 cgcgaggtcg atggcgtgct tggtgatcag cccttgggcc ttggcggtga aggccatcgc
      901 ctcggactcg tgctcggtcg attcggcctt ggccagcagc gcgcgcaccc tggccagcat
      961 ggggtcggcc gtgccggctg ccggcccact cgccgcggcc tgcgccgacg cgtagcccgg
     1021 tggtggcgcg agcagttggt cgaggcgggg tgccgagccg agcagggcga ccagctcgaa
     1081 gatgagccgc ttggcctcgt caacgggcgc gccggcggcc cagtcgtgca cccagcccgg
     1141 cgagagcgag cgcttcgacg gcgcgacgag ctgccactgt tcgacccagc gcgggtcatt
     1201 cgccgtcggg ccctggcgtg ccgcccgttc tgccccgatc gtgaggcgga tgagctcagc
     1261 acccgccttc gacccctgta gccggacgaa tcggagcagc tccgtcggct gccagccgtt
     1321 gccgtagagc tcggcggccc agaggatgag cagggcctcg gtctcggcga agaatgtggc
     1381 gggcgcgatg cgcgcagcgg ctcgcagcag cagagccacg ggctcaccgg cctgggcgtc
     1441 gcgcagcaac tcagcggtgc cgcgccgccg cggcgcgtgc cgctgtgggg tgccgaaagg
     1501 gctggccaga aactcttctt cggcgtcgga ccagtaaccg gacggccccg gccccgatgc
     1561 cttcgtgccc tgcttgtcgc gggcgcgctg ccgggcccgg tctttggccc tggcggcccg
     1621 cttgctctga ctcgcccggc tcatggcttc agcctaaacg accccatgga cagtttcttt
     1681 ctcggggcgc tgcgtggccc gccgtgcgcc acgagcccga cccaacgtct ggtgcagggg
     1741 acctctgggt gaggcgtgcg ctgggtgccg ctgcacaatc gcggactaca cagactcgtc
     1801 cagcggacgg ggacggctgt cgccggcagc catcaggcga ggatcaaccg cggactcgtc
     1861 cagtgaacgg gggacggctg tcgccggcag ccatcaggcg agggtcaacc gcggactcgt
     1921 ccaatggacg gggggcggcc ggctcgaccg cgcggttgcg tcgcccccta ccggctgtcg
     1981 gagccgacac cgtccgcgcg gactgtggcc cggaactggc gcagcacgaa ccagaccaga
     2041 cctgcgaggg tcaccagcac agctgcgacg gcgagcgcgg gggagtgctg ccacaacacg
     2101 ccgaacgcct ccaccccgac gaaccccacg gtgccgtaca gaaatgccca ggcgatcgag
     2161 cccaccacac tcgccggaag gtagcgggtg agcggcattc tggtcgctcc ggccgccagg
     2221 ttgaccaccg tctgcacccc gatggtcaag aacgagacgc tgaccgccgg tgcgccccac
     2281 cgattgatcc gatcgacggc acgccggtag ccctcggtgt tcatccaccg tgctgcccgg
     2341 gtgcgtccgg cgccgcgagc caccaggcgt ccggcccagt aggtggcgtt ggcgcgcatc
     2401 agcacgatgc agaacagcgc ggccaccacg atctcgaacg gcgcgcccca ctgggtcgga
     2461 tccacgggcc tcacctccgg acgtcgcccg ccctcagtct agaagcctgc tgagcaatca
     2521 cccgcggcgc acccggcggg gcgatctgag ccctcatatc tgggcccgga tgcccaatac
     2581 gccctcgaga ttttttttgg ggggggccag ttcatcccgc ctggaccacc gccaccaagc
     2641 cgctgatcag caggtcgagc cggcccacgc cgatctggga taccggtgcg gcggacgacg
     2701 agcgggtcgc ctggccggcc aagccgaccg cgaga
//
LOCUS       DAOSUG010000436         2726 bp    DNA     linear   ENV 10-JUN-2024
DEFINITION  MAG TPA_asm: Micropruina sp. isolate AMR_MDS_2195
            NODE_15043_length_2726_cov_4.296518, whole genome shotgun sequence.
ACCESSION   DAOSUG010000436 DAOSUG010000000
VERSION     DAOSUG010000436.1
DBLINK      BioProject: PRJNA925477
            BioSample: SAMN32864262
            Sequence Read Archive: SRR4236673
KEYWORDS    WGS; ENV; Metagenome Assembled Genome; MAG; Third Party Data; TPA;
            TPA:assembly.
SOURCE      Micropruina sp. (wastewater metagenome)
  ORGANISM  Micropruina sp.
            Bacteria; Bacillati; Actinomycetota; Actinomycetes;
            Propionibacteriales; Nocardioidaceae; Micropruina.
REFERENCE   1  (bases 1 to 2726)
  AUTHORS   Abdulkadir,N., Saraiva,J.P., Zhang,J., Stolte,S., Gillor,O.,
            Harms,H. and Rocha,U.
  TITLE     Genome-centric analyses of 165 metagenomes show that mobile genetic
            elements are crucial for the transmission of antimicrobial
            resistance genes to pathogens in activated sludge and wastewater
  JOURNAL   Microbiol Spectr 12 (3), e0291823 (2024)
   PUBMED   38289113
REFERENCE   2  (bases 1 to 2726)
  AUTHORS   Abdulkadir,N.
  TITLE     Direct Submission
  JOURNAL   Submitted (24-MAR-2023) Environmental Microbiology, Helmholtz
            Center for Environmental Research -UFZ, Permoserstr 15, Leipzig
            04318, Germany
COMMENT     The annotation was added by the NCBI Prokaryotic Genome Annotation
            Pipeline (PGAP). Information about PGAP can be found here:
            https://www.ncbi.nlm.nih.gov/genome/annotation_prok/
            
            ##Genome-Assembly-Data-START##
            Assembly Method        :: SPAdes v. 3.11.1
            Genome Representation  :: Full
            Expected Final Version :: Yes
            Genome Coverage        :: Not Applicable
            Sequencing Technology  :: Illumina MiSeq
            ##Genome-Assembly-Data-END##
            
            ##Genome-Annotation-Data-START##
            Annotation Provider               :: NCBI
            Annotation Date                   :: 05/04/2023 04:57:10
            Annotation Pipeline               :: NCBI Prokaryotic Genome
                                                 Annotation Pipeline (PGAP)
            Annotation Method                 :: Best-placed reference protein
                                                 set; GeneMarkS-2+
            Annotation Software revision      :: 6.5
            Features Annotated                :: Gene; CDS; rRNA; tRNA; ncRNA
            Genes (total)                     :: 2,776
            CDSs (total)                      :: 2,750
            Genes (coding)                    :: 2,714
            CDSs (with protein)               :: 2,714
            Genes (RNA)                       :: 26
            tRNAs                             :: 24
            ncRNAs                            :: 2
            Pseudo Genes (total)              :: 36
            CDSs (without protein)            :: 36
            Pseudo Genes (ambiguous residues) :: 0 of 36
            Pseudo Genes (frameshifted)       :: 2 of 36
            Pseudo Genes (incomplete)         :: 32 of 36
            Pseudo Genes (internal stop)      :: 2 of 36
            ##Genome-Annotation-Data-END##
FEATURES             Location/Qualifiers
     source          1..2726
                     /organism="Micropruina sp."
                     /mol_type="genomic DNA"
                     /submitter_seqid="NODE_15043_length_2726_cov_4.296518"
                     /isolate="AMR_MDS_2195"
                     /isolation_source="Activated sludge of municipal
                     wastewater treatment plants"
                     /db_xref="taxon:2737536"
                     /environmental_sample
                     /geo_loc_name="Canada"
                     /lat_lon="49.25 N 123.1 W"
                     /collection_date="2015-06-16"
                     /metagenome_source="wastewater metagenome"
                     /note="metagenomic"
     gene            245..1105
                     /locus_tag="PLF56_12355"
     CDS             245..1105
                     /locus_tag="PLF56_12355"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_020579768.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="RIO1 family regulatory kinase/ATPase"
                     /protein_id="HOQ54390.1"
                     /translation="MSSPDHAPVDWAALRYTALSEDLDEDQRWTTWRTVDRSSRGPEP
                     WPRWLVTSQAALDTELGLLKTGKEADVFLLERAVPGAESCLLAAKRYRAAEHRLFHRA
                     SSYTEGRRGRDSRENRAVRLKSGFGRQIEASRWSQAEFATLKRLWIAGVRVPYPVQVD
                     GGEVLMEFIGEGQSGRTAAPRLAQCDPGPDQLRLLWAQVVEQLIRFGTLHLVHGDLSP
                     FNVLVRGMDGAEPELVIIDVPQVLDLVSNPNGVDFLQRDCRTMAEWFVRKGLPVDPEV
                     LLAEVLGAAW"
     gene            1221..2066
                     /locus_tag="PLF56_12360"
                     /pseudo
     CDS             1221..2066
                     /locus_tag="PLF56_12360"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_002544370.1"
                     /note="incomplete; partial in the middle of a contig;
                     missing C-terminus; Derived by automated computational
                     analysis using gene prediction method: Protein Homology.
                     GO_function: GO:0004639 -
                     phosphoribosylaminoimidazolesuccinocarboxamide synthase
                     activity [Evidence IEA];
                     GO_process: GO:0006164 - purine nucleotide biosynthetic
                     process [Evidence IEA]"
                     /pseudo
                     /codon_start=1
                     /transl_table=11
                     /product="phosphoribosylaminoimidazolesuccinocarboxamide
                     synthase"
     gene            2400..2642
                     /gene="purS"
                     /locus_tag="PLF56_12365"
     CDS             2400..2642
                     /gene="purS"
                     /locus_tag="PLF56_12365"
                     /EC_number="6.3.5.3"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_013861583.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="phosphoribosylformylglycinamidine synthase
                     subunit PurS"
                     /protein_id="HOQ54391.1"
                     /translation="MARVIVDVMPKPEILDPQGKAVTGALKRLGFEGLTVRQGKRFEV
                     DIEGELTDERVAQVREAAEKLLANTVIEAFDVRVEQ"
     gene            2639..>2726
                     /locus_tag="PLF56_12370"
     CDS             2639..>2726
                     /locus_tag="PLF56_12370"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_002514428.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="phosphoribosylformylglycinamidine synthase
                     subunit PurQ"
                     /protein_id="HOQ54392.1"
                     /translation="MSRIGVVTFPGSLDDHDAQRAVKLAGHEP"
BASE COUNT          429 a          892 c          935 g          470 t
ORIGIN      
        1 aagcgtcatg tggcaaatct ggcggtaata tcggtatatc aacgggtggt gaaaaggtag
       61 ctggcgcata tgggtatcgc tgcgcgaggt ggcggcgtag actcaccagc ggtccgatga
      121 aaggaggcgt gtcatgtcga tcactgtcat gggcgcgcac gcgtccgcat tcatcgaggt
      181 ccgcattcac tgacacttcg gccgtccggc cgcccgcggg cctcctcttt acaggagaaa
      241 ccgcttgtca tcacctgatc acgcgcccgt cgactgggct gcgttgcgct ataccgccct
      301 gtccgaggac ctggacgagg accaacgctg gaccacctgg cgaacggtcg accgttcgtc
      361 ccgcggaccg gaaccctggc cgcgctggct ggtcacctcc caagccgcgc tcgacaccga
      421 actcggcctg ctcaagaccg gcaaagaggc cgacgtgttc ctgctggagc gcgccgtgcc
      481 cggagccgag tcctgccttc tcgccgcgaa gcggtaccga gccgccgagc accggctgtt
      541 tcaccgcgcg tccagttaca ccgagggtcg gcgcggtcgc gacagccgcg agaatcgtgc
      601 cgtccggctg aagtcgggct tcggccgaca gatcgaggca agccgctggt cgcaggccga
      661 gttcgccaca ctcaaacggt tgtggatcgc cggtgtgcgg gtgccgtacc cggtgcaggt
      721 ggatggggga gaggtgctga tggagttcat cggcgagggg caaagcggac gtaccgccgc
      781 gccccggctt gcccaatgcg acccgggacc ggaccaattg cgcttgttgt gggcgcaggt
      841 ggtcgagcag ttgatccggt tcggaaccct gcatctggtg catggcgacc tcagcccgtt
      901 caacgtgctg gtgcgcggca tggacggcgc cgagcccgag ttggtgatca tcgacgtgcc
      961 gcaggtgctc gacctcgtca gcaaccccaa cggcgtcgac tttctgcagc gggactgccg
     1021 caccatggcc gagtggttcg tccgcaaggg cctgccggtc gatccggagg tgctgctggc
     1081 cgaggtgctg ggtgccgcct ggtgagccgt tggtcgtcga gccgggggac tgataccgag
     1141 tgatctggcg taccgagttc ggtatgccag atcacacgct gtttgaaggc gtccggtgcg
     1201 ggatccctac actgaccagg gtgaccgatt tcgcgctgga cctgcctctc atccacgtcg
     1261 gaaaagtgcg tcgcctctac gaacttcccg acgccaccat gctgatggtg gccaccgacg
     1321 cgatctcggc ctacgaccat gtgctggcga cgccgattcc agacaagggc gccattctca
     1381 atcagctgtc cctgtggtgg ttcgagcaac tggtggaggt gccgaaccac gtgatcagca
     1441 ccgacgtgcc ggcgtcggtc cgtggccgtg cggtgatcgt cgaatcgctg gccatggtgc
     1501 cggtggaatg cgtcgcccgc gggtacctga ccgggtcggg ctgggtcgag taccagaagt
     1561 cgggcaccgt gtgcggcatc gagttgcccg cgggcctggt cgacggctcc cggctgcccg
     1621 aaccgatctt cacccctgcg atcaaggcgc ccctcggcga gcacgacgaa aacgtcgact
     1681 tcgccacggt ggctgcactg catggcgctg cactcgccgg cgcgctccgc gacctaacct
     1741 tggccatcta cgcccgggcc gaggccatcg ctcgcaaccg cggcatcatc gtggccgaca
     1801 ccaagttcga gttcggactg cgctccgacg gcacgatcgt gctggccgac gaggtgctga
     1861 cccccgactc gtcccggttc tgggacgccg ccgcgtggca gcccggggtg gcgctgccgt
     1921 ccttcgacaa gcagttcgtc cgcgactggc tgaccaagga gtccggctgg gacaaggcgt
     1981 ccgacacccc gccgccatcc ctgccggacg atgtggtggc cgccacccga gcgaaatacg
     2041 tggaggcttt cgagcggctg accggtcgca ccttccaatg acacgcccat cccggcgttg
     2101 cgtcatcccg gtgaacgccg ggcggggcag tggtgcattt gatcatcggg cggtgcgcgt
     2161 cacctggcgg tggggatctc gggcgggtag tggtgcattc gatcatccgg gcggtgcgtc
     2221 atcctggcgg atggcgggat ctcgggcggg tagtggtgca ttcgaccatc cccgccgttg
     2281 cgtcatcccg gcgaacgccg ggatctcggg ctgggtcagt cgggctgtcc ctgccggtcg
     2341 accttgtcga gacctgagag cgtccggccg attccgtccg cgccgatagg atgccggtca
     2401 tggcccgcgt gatcgtcgat gtgatgccca agcccgagat ccttgacccg cagggcaagg
     2461 ccgtcaccgg ggcgctcaag cgcctcggct tcgagggcct gaccgtgcgc caaggcaaac
     2521 ggttcgaggt cgacatcgag ggcgagctca ccgacgagcg ggtcgcccag gtgcgcgaag
     2581 ccgccgagaa actccttgcc aacaccgtga tcgaggcgtt cgacgtgcgg gtcgagcagt
     2641 gagccgcatc ggcgtcgtca cgtttcccgg gtcgctcgac gaccacgacg cccagcgcgc
     2701 ggtgaagctg gccggccacg aaccgg
//
LOCUS       DAOSUG010000437         2725 bp    DNA     linear   ENV 10-JUN-2024
DEFINITION  MAG TPA_asm: Micropruina sp. isolate AMR_MDS_2195
            NODE_15053_length_2725_cov_3.877154, whole genome shotgun sequence.
ACCESSION   DAOSUG010000437 DAOSUG010000000
VERSION     DAOSUG010000437.1
DBLINK      BioProject: PRJNA925477
            BioSample: SAMN32864262
            Sequence Read Archive: SRR4236673
KEYWORDS    WGS; ENV; Metagenome Assembled Genome; MAG; Third Party Data; TPA;
            TPA:assembly.
SOURCE      Micropruina sp. (wastewater metagenome)
  ORGANISM  Micropruina sp.
            Bacteria; Bacillati; Actinomycetota; Actinomycetes;
            Propionibacteriales; Nocardioidaceae; Micropruina.
REFERENCE   1  (bases 1 to 2725)
  AUTHORS   Abdulkadir,N., Saraiva,J.P., Zhang,J., Stolte,S., Gillor,O.,
            Harms,H. and Rocha,U.
  TITLE     Genome-centric analyses of 165 metagenomes show that mobile genetic
            elements are crucial for the transmission of antimicrobial
            resistance genes to pathogens in activated sludge and wastewater
  JOURNAL   Microbiol Spectr 12 (3), e0291823 (2024)
   PUBMED   38289113
REFERENCE   2  (bases 1 to 2725)
  AUTHORS   Abdulkadir,N.
  TITLE     Direct Submission
  JOURNAL   Submitted (24-MAR-2023) Environmental Microbiology, Helmholtz
            Center for Environmental Research -UFZ, Permoserstr 15, Leipzig
            04318, Germany
COMMENT     The annotation was added by the NCBI Prokaryotic Genome Annotation
            Pipeline (PGAP). Information about PGAP can be found here:
            https://www.ncbi.nlm.nih.gov/genome/annotation_prok/
            
            ##Genome-Assembly-Data-START##
            Assembly Method        :: SPAdes v. 3.11.1
            Genome Representation  :: Full
            Expected Final Version :: Yes
            Genome Coverage        :: Not Applicable
            Sequencing Technology  :: Illumina MiSeq
            ##Genome-Assembly-Data-END##
            
            ##Genome-Annotation-Data-START##
            Annotation Provider               :: NCBI
            Annotation Date                   :: 05/04/2023 04:57:10
            Annotation Pipeline               :: NCBI Prokaryotic Genome
                                                 Annotation Pipeline (PGAP)
            Annotation Method                 :: Best-placed reference protein
                                                 set; GeneMarkS-2+
            Annotation Software revision      :: 6.5
            Features Annotated                :: Gene; CDS; rRNA; tRNA; ncRNA
            Genes (total)                     :: 2,776
            CDSs (total)                      :: 2,750
            Genes (coding)                    :: 2,714
            CDSs (with protein)               :: 2,714
            Genes (RNA)                       :: 26
            tRNAs                             :: 24
            ncRNAs                            :: 2
            Pseudo Genes (total)              :: 36
            CDSs (without protein)            :: 36
            Pseudo Genes (ambiguous residues) :: 0 of 36
            Pseudo Genes (frameshifted)       :: 2 of 36
            Pseudo Genes (incomplete)         :: 32 of 36
            Pseudo Genes (internal stop)      :: 2 of 36
            ##Genome-Annotation-Data-END##
FEATURES             Location/Qualifiers
     source          1..2725
                     /organism="Micropruina sp."
                     /mol_type="genomic DNA"
                     /submitter_seqid="NODE_15053_length_2725_cov_3.877154"
                     /isolate="AMR_MDS_2195"
                     /isolation_source="Activated sludge of municipal
                     wastewater treatment plants"
                     /db_xref="taxon:2737536"
                     /environmental_sample
                     /geo_loc_name="Canada"
                     /lat_lon="49.25 N 123.1 W"
                     /collection_date="2015-06-16"
                     /metagenome_source="wastewater metagenome"
                     /note="metagenomic"
     gene            345..938
                     /locus_tag="PLF56_12375"
     CDS             345..938
                     /locus_tag="PLF56_12375"
                     /inference="COORDINATES: protein motif:HMM:NF017454.2"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="type VI secretion system tube protein Hcp"
                     /protein_id="HOQ54393.1"
                     /translation="MNAPVHASRRSLLVGGGLGAAGLLNLGGADLAGAAPMAFVPPGH
                     ADGGVDWWLLLDGIAGESTDRDHPKQLKVRAMDWQAVQKATSASGASSKDISLAFDTS
                     AASPLILKRCVQGSAVRTARLSGRKTGEGQRDFLWWDFTDVRFRSYDVAFTDGAPLDV
                     VTFSFTQVTMTYRPQDAKGGLGTPIAVTWNLTTDKVS"
     gene            complement(943..1296)
                     /locus_tag="PLF56_12380"
     CDS             complement(943..1296)
                     /locus_tag="PLF56_12380"
                     /inference="COORDINATES: ab initio
                     prediction:GeneMarkS-2+"
                     /note="Derived by automated computational analysis using
                     gene prediction method: GeneMarkS-2+."
                     /codon_start=1
                     /transl_table=11
                     /product="hypothetical protein"
                     /protein_id="HOQ54394.1"
                     /translation="MPDSSQPAAQPTWLPAALLGLMVAACLLAGLLLSGRAVDHYVLP
                     APGGGVQAMTHTYVERVVLAWLAWAGALASAGALVLRLLGRTIARWAWIVLGAVLLIV
                     LNAALLVSALPQATY"
     gene            complement(1300..2676)
                     /locus_tag="PLF56_12385"
     CDS             complement(1300..2676)
                     /locus_tag="PLF56_12385"
                     /inference="COORDINATES: protein motif:HMM:NF004561.0"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_function: GO:0003677 - DNA binding [Evidence IEA];
                     GO_function: GO:0016987 - sigma factor activity [Evidence
                     IEA];
                     GO_process: GO:0006352 - DNA-templated transcription
                     initiation [Evidence IEA];
                     GO_process: GO:0006355 - regulation of DNA-templated
                     transcription [Evidence IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="RNA polymerase sigma factor"
                     /protein_id="HOQ54395.1"
                     /translation="MSPRSTSAAKADIVSDEAAPPRRTRAATPPKQPAARRGRKPAVV
                     AVAATPEVDAGEAGTDAPQDIEVEAVEAELEEPTLEEVVLSPEAVDVKFAREGDDVVL
                     TVGGKRRSLDDVDDSTFEEAKEAVVEKELTEDQGFIVSDADDADEPEQQVLVAGATAD
                     PVKDYLKQIGKVALLNAEQEVTLAKRIEAGLFAEESMNTARSLSATDGDDYQWISDDG
                     RRAKNHLLEANLRLVVSLAKRYTGRGMLFLDLIQEGNLGLIRAVEKFDYTKGYKFSTY
                     ATWWIKQAITRAMADQARTIRIPVHMVEVINKLARVQRQMLQDLGREPTPEELAKELD
                     MTPEKVVEVQKYGREPISLHTPLGEDGDSEFGDLIEDSEAVVPAEAVNFTLLQEQLHD
                     VLDTLSEREAGVVSMRFGLTDGQPKTLDEIGKVYGVTRERIRQIESKTMSKLRHPSRS
                     QVLRDYLD"
BASE COUNT          419 a          922 c          925 g          459 t
ORIGIN      
        1 gcggctgctc ggggcgttca acgcggcgct cgacgactga ggcgcgcccg accgggctca
       61 cccggatcac gcccacggca ccccccttgc tcagagcggc gctcgacgac tgaggcgtgc
      121 tcgaccctgc cccacccgga tcacgcccac ggcacccccc ttgctcagcg cggcgctcga
      181 cgtccgagcc gtgctcgacc gtgaccaccc ggaccccctg agttcaccgc gatcggtgcg
      241 cagccgagcc ggcgcacatt ggtgttgccg ggcttggcga cggccggtat caccgggacc
      301 ggtgggcgct cctgaccacc acgaatcgat tctctggagg acccatgaac gctcccgtcc
      361 acgcgtcccg gcgctccctg ctcgtcggcg gcggcctcgg cgcggccgga ctgctcaatc
      421 tgggtggtgc cgacctcgcc ggcgccgcac cgatggcatt cgtccccccg ggtcacgccg
      481 acggcggcgt cgactggtgg ctgctgctcg acggtatcgc cggtgagtcg acggaccgcg
      541 accacccgaa acaactcaag gtcagggcga tggactggca ggccgttcag aaggccacca
      601 gcgcgtccgg agccagcagc aaggacatct ccctggcctt cgacaccagc gcggcgtctc
      661 cgctgatcct gaagagatgc gtgcagggat cggccgttcg cacggcgcgg ctcagtggcc
      721 gcaagactgg tgaggggcag cgcgactttc tgtggtggga cttcaccgac gtgcggttca
      781 ggtcctacga tgtcgccttc accgacggcg caccgctcga cgtggtcacg ttctcgttca
      841 cgcaggtcac catgacctac cggccacagg acgccaaggg tggcctgggc acgccgatcg
      901 cggtcacttg gaacctcacg accgacaagg tcagctgagg catcagtagg tggcttgcgg
      961 cagggccgag acgagcagtg ccgcattcag cacgatcagc aggacggcgc cgagcacgat
     1021 ccacgcccaa cgcgcgatgg ttcgtccgag caaccggagc acgagcgcac ccgccgaggc
     1081 cagcgcgccc gcccaggcga gccacgcgag aaccacccgc tcgacgtacg tgtgcgtcat
     1141 cgcctggacg ccgccgccgg gcgccggcag cacgtagtgg tcgacggcgc ggccggacaa
     1201 cagaagcccc gcaagcaggc aggccgccac catcaggccc agcagcgcgg cgggcagcca
     1261 ggtgggctgc gccgccggct gggacgaatc cggcaccggt cagtccaggt agtcgcgcag
     1321 cacctgcgac cgggacggat gccgcagctt gctcatcgtc ttggactcga tctggcggat
     1381 acgctcgcgg gtgaccccgt agaccttgcc gatctcatcc agggtcttgg gctggccgtc
     1441 ggtgagcccg aagcgcatgc tgaccacgcc cgcctcgcgc tccgacaagg tgtcgagcac
     1501 gtcatgcagt tgctcttgca gcagggtgaa gttcaccgct tcggcaggaa ccaccgcctc
     1561 ggagtcctcg atcaggtcgc cgaactccga gtcgccgtct tcgccgagcg gggtgtgcag
     1621 cgagatcggc tcacgaccgt acttctgcac ctcgacgacc ttctcgggcg tcatgtcgag
     1681 ctctttggcc aactcttcgg gcgttggttc ccggcccaag tcctgcagca tctgccgctg
     1741 gacgcgcgcg agcttgttga tcacctcgac catgtgcacc gggatgcgga tggtgcgggc
     1801 ctggtcggcc atggcgcggg tgatggcctg cttgatccac caggtggcgt aggtcgagaa
     1861 cttgtagccc ttggtgtagt cgaacttctc gaccgcacgg atcaggccga ggttgccctc
     1921 ttggatcagg tcgagaaaca gcatgccgcg gccggtgtag cgcttggcga gcgacaccac
     1981 gagacgcagg ttggcctcga gcaggtggtt cttggcgcga cgaccgtcgt ccgagatcca
     2041 ttggtagtca tcgccatcgg tggcggaaag cgaccgcgcg gtgttcatcg attcttcggc
     2101 gaacaggccg gcctcgatgc gtttggcgag ggtgacctcc tgctcggcgt tcagcagggc
     2161 gaccttgccg atctgcttga ggtagtcctt gaccgggtcg gcggtggcgc ccgccaccag
     2221 aacctgctgc tcgggctcgt cggcgtcgtc ggcgtcggag acgatgaagc cctggtcttc
     2281 ggtcagttcc ttctcgacga cggcctcttt ggcctcttcg aaggtcgaat cgtcgacgtc
     2341 gtcgagtgag cgacgcttgc cgccgacggt cagcacgacg tcatcgccct cgcgagcgaa
     2401 cttgacgtcg acggcctcgg ggctgagcac aacctcttcg agagtgggct cttcgagttc
     2461 agcctcaacg gcctcgacct cgatgtcttg gggagcgtcg gtcccggctt caccggcatc
     2521 gacctcggga gtggcggcga cggcgacgac cgcaggctta cggccacgcc gggcggcggg
     2581 ctgcttcggc ggcgtggcgg cgcgggtacg gcgggggggt gcagcttcgt ccgatacgat
     2641 gtcagccttg gctgctgagg tggaacgtgg tgacacaagt agcctttcgg attcttggtt
     2701 ttgggcgcac gccatcgggg tgttg
//
LOCUS       DAOSUG010000438         2724 bp    DNA     linear   ENV 10-JUN-2024
DEFINITION  MAG TPA_asm: Micropruina sp. isolate AMR_MDS_2195
            NODE_15058_length_2724_cov_4.340952, whole genome shotgun sequence.
ACCESSION   DAOSUG010000438 DAOSUG010000000
VERSION     DAOSUG010000438.1
DBLINK      BioProject: PRJNA925477
            BioSample: SAMN32864262
            Sequence Read Archive: SRR4236673
KEYWORDS    WGS; ENV; Metagenome Assembled Genome; MAG; Third Party Data; TPA;
            TPA:assembly.
SOURCE      Micropruina sp. (wastewater metagenome)
  ORGANISM  Micropruina sp.
            Bacteria; Bacillati; Actinomycetota; Actinomycetes;
            Propionibacteriales; Nocardioidaceae; Micropruina.
REFERENCE   1  (bases 1 to 2724)
  AUTHORS   Abdulkadir,N., Saraiva,J.P., Zhang,J., Stolte,S., Gillor,O.,
            Harms,H. and Rocha,U.
  TITLE     Genome-centric analyses of 165 metagenomes show that mobile genetic
            elements are crucial for the transmission of antimicrobial
            resistance genes to pathogens in activated sludge and wastewater
  JOURNAL   Microbiol Spectr 12 (3), e0291823 (2024)
   PUBMED   38289113
REFERENCE   2  (bases 1 to 2724)
  AUTHORS   Abdulkadir,N.
  TITLE     Direct Submission
  JOURNAL   Submitted (24-MAR-2023) Environmental Microbiology, Helmholtz
            Center for Environmental Research -UFZ, Permoserstr 15, Leipzig
            04318, Germany
COMMENT     The annotation was added by the NCBI Prokaryotic Genome Annotation
            Pipeline (PGAP). Information about PGAP can be found here:
            https://www.ncbi.nlm.nih.gov/genome/annotation_prok/
            
            ##Genome-Assembly-Data-START##
            Assembly Method        :: SPAdes v. 3.11.1
            Genome Representation  :: Full
            Expected Final Version :: Yes
            Genome Coverage        :: Not Applicable
            Sequencing Technology  :: Illumina MiSeq
            ##Genome-Assembly-Data-END##
            
            ##Genome-Annotation-Data-START##
            Annotation Provider               :: NCBI
            Annotation Date                   :: 05/04/2023 04:57:10
            Annotation Pipeline               :: NCBI Prokaryotic Genome
                                                 Annotation Pipeline (PGAP)
            Annotation Method                 :: Best-placed reference protein
                                                 set; GeneMarkS-2+
            Annotation Software revision      :: 6.5
            Features Annotated                :: Gene; CDS; rRNA; tRNA; ncRNA
            Genes (total)                     :: 2,776
            CDSs (total)                      :: 2,750
            Genes (coding)                    :: 2,714
            CDSs (with protein)               :: 2,714
            Genes (RNA)                       :: 26
            tRNAs                             :: 24
            ncRNAs                            :: 2
            Pseudo Genes (total)              :: 36
            CDSs (without protein)            :: 36
            Pseudo Genes (ambiguous residues) :: 0 of 36
            Pseudo Genes (frameshifted)       :: 2 of 36
            Pseudo Genes (incomplete)         :: 32 of 36
            Pseudo Genes (internal stop)      :: 2 of 36
            ##Genome-Annotation-Data-END##
FEATURES             Location/Qualifiers
     source          1..2724
                     /organism="Micropruina sp."
                     /mol_type="genomic DNA"
                     /submitter_seqid="NODE_15058_length_2724_cov_4.340952"
                     /isolate="AMR_MDS_2195"
                     /isolation_source="Activated sludge of municipal
                     wastewater treatment plants"
                     /db_xref="taxon:2737536"
                     /environmental_sample
                     /geo_loc_name="Canada"
                     /lat_lon="49.25 N 123.1 W"
                     /collection_date="2015-06-16"
                     /metagenome_source="wastewater metagenome"
                     /note="metagenomic"
     gene            <1..949
                     /locus_tag="PLF56_12390"
     CDS             <1..949
                     /locus_tag="PLF56_12390"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_002514992.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=2
                     /transl_table=11
                     /product="alpha-1,4-glucan--maltose-1-phosphate
                     maltosyltransferase"
                     /protein_id="HOQ54396.1"
                     /translation="GSIAYAENPPKKYQDIYPINFDNDPVGIYNEVLRLFKLWMSHGV
                     RIFRIDNPHTKPVEFWGWIIKRLRETDPDVLFLAEAFTKPEMMAALGKVGFHQSYTYF
                     TWRTTKYELTEYAVELANETAPFYRPNFFVNTQDINPFHLQSGNHAMFGIRAILAATL
                     SPTWGVYSGFELFEHEALAPGREEYLNSEKYEYRPRDYNAEPNLNLLFGTLNSIRRAH
                     PALQQLRQLHFHHAGHDGVIAYSKRDADDVVIVICSLDPDNQVESDVWLDLGALGVSG
                     DSVPLRDELTGAEFTWGERNFVRLNPLTPAHILHVLPTR"
     gene            1009..2334
                     /locus_tag="PLF56_12395"
     CDS             1009..2334
                     /locus_tag="PLF56_12395"
                     /inference="COORDINATES: ab initio
                     prediction:GeneMarkS-2+"
                     /note="Derived by automated computational analysis using
                     gene prediction method: GeneMarkS-2+."
                     /codon_start=1
                     /transl_table=11
                     /product="phosphotransferase"
                     /protein_id="HOQ54397.1"
                     /translation="MSAQSLAAWWGLISGARWFGGKGRSASLTAVTPLPWLTPAGSLP
                     RVRGEIATITYAEGRTEQYLLLAGYTANPEAPVVTTVADPEHGTLSVVEAPGDPGCVR
                     ALLTALVDPAHPAMQWVDASAIDPSEPTTIWPGEQSNTTLCLGDHHMLKLFRRLEAGR
                     NLDVEVLAALNGSGITPALYGTLVGDLPAGARADLGMFVERVQGVEDGWVWACQACAA
                     DRDISVEAMQLGAALRAVHERLASAFGTSVEPGAEVADQIRLRFDAARAEIDELALID
                     GAVESTLQALARTDLVTQRVHGDFHLGQTLRSANGWTIIDFEGEPLKSLSERRRPDSV
                     WRDVAGMLRSFDYVRAAHPDPTSAPAAAWSAAARHAFLDGYCQGASPDSGVLDAYELD
                     KAVYEVVYETRNRPDWAHIPLGAVHNVARRTAPSPTHDSPSRTHNSKEN"
     gene            2334..>2724
                     /locus_tag="PLF56_12400"
     CDS             2334..>2724
                     /locus_tag="PLF56_12400"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_014846727.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="1,4-alpha-glucan branching enzyme"
                     /protein_id="HOQ54398.1"
                     /translation="MAHDSAGGLTGWDLEGFHSGGDTELWRRLGAHQVTIHDDERGEI
                     SGTRFAVWAPNAQAVRLNADFNWWTGDAMSLVPGSGVWAIFVEGVGDGALYKYNILGG
                     DGVWREKVDPMAFFAEKAPANSSIVYTS"
BASE COUNT          459 a          943 c          885 g          437 t
ORIGIN      
        1 cggctcgatc gcctacgccg agaacccgcc caagaagtac caggacatct acccgatcaa
       61 cttcgacaac gacccggtgg gcatctacaa cgaggtgctg cggctgttca agctgtggat
      121 gagccacggt gtgcgcatct tccgcatcga caacccgcac accaagccgg tcgagttctg
      181 gggctggatc atcaagcggc tgcgcgaaac cgatccggac gttctgttcc tggccgaggc
      241 gttcaccaag cccgagatga tggccgcgct gggcaaggtc gggtttcatc agagctacac
      301 ctacttcacc tggcgcacca ccaagtacga actcaccgag tacgcggtcg aactggccaa
      361 cgagacggcg ccgttctacc ggcccaactt cttcgtcaac acccaagaca tcaacccgtt
      421 ccatctgcag tcgggcaacc acgcgatgtt cggaatccgg gcgatcctgg ccgccacgct
      481 gtcgcccacc tggggtgtgt actcgggctt cgagttgttc gagcacgagg cgctggcgcc
      541 ggggcgcgaa gagtacctga actccgagaa gtacgagtac cgcccgcgcg actacaacgc
      601 cgagcccaac ctgaacttgc tgttcggcac cctgaactcg attcggcggg cgcaccctgc
      661 gctgcagcag ttgcggcaac tgcactttca tcacgccggc cacgacgggg tgatcgccta
      721 ctccaagcgc gacgcggacg acgtggtgat cgtgatctgc agcctcgacc ccgacaacca
      781 ggtcgaatcc gacgtctggc tcgatctggg cgcgctgggt gtgagcggcg actccgtgcc
      841 actgcgtgac gagctgaccg gcgccgagtt cacctggggc gagcgcaact tcgtccggtt
      901 gaacccgctg acacccgccc acatcctgca cgtgctgccc acccgctgag ccccgtccat
      961 gcggtgtaaa agctgttgtc cgacgcactt tcctgacagg atggccaggt gagcgcacag
     1021 tcgttggcgg cgtggtgggg cctgatttcg ggcgcgcgct ggttcggcgg caagggtcgg
     1081 tcggccagcc tcacggcagt cacgccgctg ccgtggttga ccccggccgg ttcgctgccg
     1141 cgcgtgcgcg gcgagatcgc cacgatcacc tatgccgagg gccgcaccga gcagtacctg
     1201 ctgctggccg ggtacaccgc gaaccccgag gcgccagtgg tcacgaccgt tgccgacccc
     1261 gagcacggca ccctgagcgt ggtcgaggcg ccgggtgatc cggggtgcgt gcgggcgttg
     1321 ctgacggccc tggtcgatcc ggctcacccg gcgatgcagt gggtcgacgc gtcggcgatc
     1381 gacccgtccg agccgaccac gatctggccc ggcgaacaga gcaacaccac gctctgcctg
     1441 ggcgatcacc acatgctcaa gctgttccgc aggctcgaag cgggtcgcaa cctcgacgtc
     1501 gaggtgctgg ctgcgctgaa cggctcgggc atcaccccgg cgctgtacgg caccctggtc
     1561 ggtgacctgc ccgccggcgc gcgggccgat ctgggcatgt tcgtcgagcg cgtccagggc
     1621 gtcgaggacg gctgggtgtg ggcctgtcag gcgtgcgccg ccgatcgtga catcagcgtc
     1681 gaagccatgc agctgggcgc tgccctgcgc gcggtgcacg agcggttggc cagcgccttc
     1741 ggcacgtccg tcgagccggg cgccgaggtg gccgaccaga ttcggctgcg cttcgacgcc
     1801 gcccgggccg agatcgacga actggcgctc atcgatggcg cggtcgagtc caccctgcag
     1861 gcgctcgcgc gcaccgacct ggtcacgcag cgcgtccacg gagactttca cctgggccag
     1921 acgctgcggt ccgcgaacgg ttggacgatc atcgacttcg agggcgaacc gttgaagagc
     1981 ttgagcgagc gacgccgtcc cgacagtgtg tggcgcgacg tcgccggcat gctgcgctcc
     2041 ttcgactacg tgcgcgcggc ccaccccgat cccacctctg ccccagccgc ggcctggtcc
     2101 gctgctgcca ggcacgcatt cctcgacggc tattgccaag gcgccagtcc cgactccggc
     2161 gtgctcgacg cctacgagct cgacaaggcc gtctacgagg tggtgtacga aacccgaaat
     2221 cgacccgact gggcacacat cccgctcggc gccgtccaca atgtggcaag gcggacggcg
     2281 ccttccccca cccacgattc accttcccgc acccacaatt caaaggagaa ctgatggcac
     2341 acgattcagc cggtggcctg accggatggg acctcgaagg cttccacagc ggcggcgaca
     2401 ccgagctgtg gcgtcggctc ggcgcccatc aggtgaccat tcatgacgac gagcggggtg
     2461 agatcagtgg cacccggttc gcggtctggg cccccaacgc gcaggcggta cggctcaacg
     2521 ccgacttcaa ctggtggacc ggcgatgcca tgtcgctggt gcccggctcg ggtgtctggg
     2581 cgatcttcgt cgagggcgtc ggcgacggcg cgctctataa gtacaacatt ctcggcggcg
     2641 acggggtgtg gcgcgaaaaa gtcgacccga tggcgttctt cgccgaaaag gcgcccgcca
     2701 acagctcgat cgtctacacc agcc
//
LOCUS       DAOSUG010000439         2724 bp    DNA     linear   ENV 10-JUN-2024
DEFINITION  MAG TPA_asm: Micropruina sp. isolate AMR_MDS_2195
            NODE_15059_length_2724_cov_4.204946, whole genome shotgun sequence.
ACCESSION   DAOSUG010000439 DAOSUG010000000
VERSION     DAOSUG010000439.1
DBLINK      BioProject: PRJNA925477
            BioSample: SAMN32864262
            Sequence Read Archive: SRR4236673
KEYWORDS    WGS; ENV; Metagenome Assembled Genome; MAG; Third Party Data; TPA;
            TPA:assembly.
SOURCE      Micropruina sp. (wastewater metagenome)
  ORGANISM  Micropruina sp.
            Bacteria; Bacillati; Actinomycetota; Actinomycetes;
            Propionibacteriales; Nocardioidaceae; Micropruina.
REFERENCE   1  (bases 1 to 2724)
  AUTHORS   Abdulkadir,N., Saraiva,J.P., Zhang,J., Stolte,S., Gillor,O.,
            Harms,H. and Rocha,U.
  TITLE     Genome-centric analyses of 165 metagenomes show that mobile genetic
            elements are crucial for the transmission of antimicrobial
            resistance genes to pathogens in activated sludge and wastewater
  JOURNAL   Microbiol Spectr 12 (3), e0291823 (2024)
   PUBMED   38289113
REFERENCE   2  (bases 1 to 2724)
  AUTHORS   Abdulkadir,N.
  TITLE     Direct Submission
  JOURNAL   Submitted (24-MAR-2023) Environmental Microbiology, Helmholtz
            Center for Environmental Research -UFZ, Permoserstr 15, Leipzig
            04318, Germany
COMMENT     The annotation was added by the NCBI Prokaryotic Genome Annotation
            Pipeline (PGAP). Information about PGAP can be found here:
            https://www.ncbi.nlm.nih.gov/genome/annotation_prok/
            
            ##Genome-Assembly-Data-START##
            Assembly Method        :: SPAdes v. 3.11.1
            Genome Representation  :: Full
            Expected Final Version :: Yes
            Genome Coverage        :: Not Applicable
            Sequencing Technology  :: Illumina MiSeq
            ##Genome-Assembly-Data-END##
            
            ##Genome-Annotation-Data-START##
            Annotation Provider               :: NCBI
            Annotation Date                   :: 05/04/2023 04:57:10
            Annotation Pipeline               :: NCBI Prokaryotic Genome
                                                 Annotation Pipeline (PGAP)
            Annotation Method                 :: Best-placed reference protein
                                                 set; GeneMarkS-2+
            Annotation Software revision      :: 6.5
            Features Annotated                :: Gene; CDS; rRNA; tRNA; ncRNA
            Genes (total)                     :: 2,776
            CDSs (total)                      :: 2,750
            Genes (coding)                    :: 2,714
            CDSs (with protein)               :: 2,714
            Genes (RNA)                       :: 26
            tRNAs                             :: 24
            ncRNAs                            :: 2
            Pseudo Genes (total)              :: 36
            CDSs (without protein)            :: 36
            Pseudo Genes (ambiguous residues) :: 0 of 36
            Pseudo Genes (frameshifted)       :: 2 of 36
            Pseudo Genes (incomplete)         :: 32 of 36
            Pseudo Genes (internal stop)      :: 2 of 36
            ##Genome-Annotation-Data-END##
FEATURES             Location/Qualifiers
     source          1..2724
                     /organism="Micropruina sp."
                     /mol_type="genomic DNA"
                     /submitter_seqid="NODE_15059_length_2724_cov_4.204946"
                     /isolate="AMR_MDS_2195"
                     /isolation_source="Activated sludge of municipal
                     wastewater treatment plants"
                     /db_xref="taxon:2737536"
                     /environmental_sample
                     /geo_loc_name="Canada"
                     /lat_lon="49.25 N 123.1 W"
                     /collection_date="2015-06-16"
                     /metagenome_source="wastewater metagenome"
                     /note="metagenomic"
     gene            complement(<1..936)
                     /locus_tag="PLF56_12405"
     CDS             complement(<1..936)
                     /locus_tag="PLF56_12405"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_015069572.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="oxidoreductase"
                     /protein_id="HOQ54399.1"
                     /translation="MSYSKRLLERERATGRPVRVGVVGAGQMGSGLVAQINRVPGLVM
                     AAVADIDVTRAQTALAGAGVEHVTSTDDLTDASVAIENGESVALSDGLALTKLPLDVI
                     VEVSGVPDVAAEIAYAGLLAGKHTLLMTVEADITVGLLLAGMGNKPGGPIYAVCRGDE
                     PPECLKLIEYAEDIGLQVVVAGKGKNNPMRPTDTPTDVAAEARAKGMNPKMLCSFTDG
                     SKTQLEMCALSNATGYPVDVPGMHGIPCSVDELAAKLVPEAVGGVMSSEGPFVEYVTG
                     NVAPGVFVIAKSTNDVVTHELDYLKLGKGPYYALYR"
     gene            complement(933..1373)
                     /locus_tag="PLF56_12410"
     CDS             complement(933..1373)
                     /locus_tag="PLF56_12410"
                     /inference="COORDINATES: protein motif:HMM:NF018611.2"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="transcriptional regulator GutM"
                     /protein_id="HOQ54400.1"
                     /translation="MGWEFPAILGGALVVSLLLGLWQQARYARSVNEMVQKHHGSGRI
                     LVTGRGLGRLRGTIVMLVIDDAADQAVAASRLRGSTIFAVAKDAPELIGPVADLPERA
                     GDKQTRKAIDMALTQLKATRARVSDNRILTPPTQSGSAKRKVQR"
     gene            complement(1377..1748)
                     /locus_tag="PLF56_12415"
     CDS             complement(1377..1748)
                     /locus_tag="PLF56_12415"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_016666971.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_component: GO:0005737 - cytoplasm [Evidence IEA];
                     GO_function: GO:0008982 -
                     protein-N(PI)-phosphohistidine-sugar phosphotransferase
                     activity [Evidence IEA];
                     GO_process: GO:0009401 - phosphoenolpyruvate-dependent
                     sugar phosphotransferase system [Evidence IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="PTS glucitol/sorbitol transporter subunit IIA"
                     /protein_id="HOQ54401.1"
                     /translation="MANELWSATIDRIGEFTAEMFDGGVFILFGDPVPDALGEVSIVH
                     HTRQAAARDIRAGDTIELGGVVLTLDEVGERANLNLTDLGHAVIYVNSPEQPLLPGAI
                     KVSGEARPHPTTGSAIVIRGE"
     gene            complement(2007..>2724)
                     /locus_tag="PLF56_12420"
     CDS             complement(2007..>2724)
                     /locus_tag="PLF56_12420"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_016666970.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=2
                     /transl_table=11
                     /product="PTS sorbitol transporter subunit IIB"
                     /protein_id="HOQ54402.1"
                     /translation="WSAGPVITEDIFVSAVTPNEVSLAGEETMAQPPVSAQVDALDAD
                     RARYEAQKAEAKVAAKQAGGFTGMVVSFGEAIGYFINTLLSSGRQAMKLVLNTIIPFM
                     AYVSLLIGIVTYTHVSEWIAQGVTPLVSNPFGLILLGVITAIPILSPILGPGAAIAQV
                     VGVLMGQQIAAGALPVQYALPTLFAINGQVGCDFVPVGLSLGEAEPETVSVGVPAVLF
                     SRLITSPVAVIIAYLASFAI"
BASE COUNT          427 a          916 c          922 g          459 t
ORIGIN      
        1 ccggtacagg gcgtagtagg gacccttgcc caacttgagg tagtcgagtt catgggtgac
       61 cacgtcgttg gtcgacttgg cgatcacgaa cacgccgggt gccacgttgc cggtgacgta
      121 ctcgacgaac gggccttcgc tgctcatcac tcctccgacc gcttcgggca ccagcttggc
      181 ggccaactcg tccacggagc aagggatgcc gtgcatgccg ggcacgtcca ccgggtagcc
      241 ggtggcgttg ctcagcgcgc acatctccaa ctgggtcttc gacccgtcgg tgaacgagca
      301 cagcatcttg gggttcatgc ccttggccct ggcctcagcg gccacatcgg tgggggtgtc
      361 ggtgggccgc atcgggttgt tcttgccctt gccggccacc accacctgca ggccgatgtc
      421 ttcggcgtac tcgatcagct tgaggcactc gggcggctcg tcgccgcggc agaccgcata
      481 gatgggcccg cccggcttgt tgcccatgcc ggccagcagc agtccgacgg tgatgtcggc
      541 ctcgacggtc atcaacaatg tgtgcttgcc ggccagcagg ccggcgtagg cgatctcggc
      601 ggccacgtcg ggcacgcccg agacctcgac gatcacgtcg agcggcagct tggtcagggc
      661 caggccgtcg ctcagcgcga cggattcgcc gttctcgatc gcgaccgagg cgtcggtcag
      721 gtcgtcggtg ctcgtgacgt gctcaacgcc ggctccggcc agagcggtct gggcgcgcgt
      781 gacgtcgatg tcggcgaccg ccgccatgac caggcccggc acccggttga tctgggcgac
      841 gagcccggac cccatctgcc cggcaccgac gacaccgacc cgtaccggac gtccggtggc
      901 ccgctcacgt tcgagcagcc gtttgctgta actcatcgtt gaaccttccg cttggccgag
      961 cccgactggg tcggcggggt gagaatcctg ttgtcgctga cccgggcccg ggtggccttc
     1021 agttgcgtca gggccatgtc gatggctttg cgggtctgct tgtcaccggc acgttcgggc
     1081 aggtcggcga ccgggccgat cagctcgggt gcgtccttgg ccacggcgaa gatcgtcgag
     1141 ccgcgcagcc tgctggcggc gaccgcctgg tcggcggcgt cgtcgatgac gagcatcacg
     1201 atggtgcccc gcagcctgcc caggccgcgg ccggtgacga ggatccggcc gctgccgtgg
     1261 tgcttctgca ccatctcgtt gaccgaacgt gcgtagcggg cctgctgcca caggccgagc
     1321 agcagcgaca ccaccagagc gccgccgaga atcgcgggaa actcccagcc cacggctcac
     1381 tccccccgga tcacgatggc cgagcctgtc gtggggtgcg gacgggcctc gcccgagacc
     1441 ttgatcgcac ccggcagcag gggctgctcg gggctgttca catagatcac ggcgtggccc
     1501 agatcggtga ggttcaggtt ggcccgttcg cccacctcgt ccagggtgag cacgacgccg
     1561 ccgagttcga tggtgtcgcc ggcgcgaatg tcgcgcgcgg cggcctgccg ggtgtggtgg
     1621 acgatgctga cctcgcccag ggcgtccggc accgggtcgc cgaacaggat gaacaccccg
     1681 ccgtcgaaca tttcagcggt gaactcaccg atccgatcga tggtggcgga ccacagttcg
     1741 tttgccatgg gtatcagtcc ttgtctgttg atcgtggggt ggagcttggc ccggtggggc
     1801 cgctggtcga gcctgtcgaa acccgagccc gctggtccga gcctgtcgag acccgagccc
     1861 gctggtcgag cctgtcgaga cccacaccgt aggtcgagct tgtcgaatcc gagcccgtcg
     1921 aaaccggcgg cctgatcgcc tctgaccatg actgcggtgt caacacgctc aaccgcggtg
     1981 actcgacaag ctcaaccatc ggtgcttcag atcgcgaacg acgccaggta ggcgatgatg
     2041 accgcgacgg gtgaggtgat caggcggctg aacaacaccg ccggcacgcc gaccgagacg
     2101 gtttctggct ccgcctcgcc gagcgacagc ccgaccggta cgaagtcaca gccgacctgg
     2161 ccgttgatgg cgaacagggt gggcagcgcg tactgcaccg gcaacgcgcc cgccgcgatc
     2221 tgctggccca tcagcacgcc gaccacctgc gcgatcgccg cacccgggcc caggatgggc
     2281 gacaggatcg ggattgcggt gatcacaccg agcaggatca ggccgaacgg gttgctgacc
     2341 aggggcgtca cgccttgggc gatccattcc gacacgtggg tgtaggtgac gatgccgatc
     2401 agcagcgaca cgtaggccat gaacggaatg atcgtgttca gcaccagctt catcgcctgc
     2461 cgaccggagc tcagcagggt gttgatgaag tagccgatcg cctcgccgaa gctcaccacc
     2521 atgccggtga agccgccggc ctgtttggcc gcaaccttcg cctcggcctt ctgggcctca
     2581 tagcgggcac ggtcggcgtc cagggcgtcc acctgcgcgg agaccggcgg ctgcgccatc
     2641 gtctcttcac cggcgaggct gacctcgttg ggcgtcaccg ccgagacgaa gatgtcttcg
     2701 gtgataactg ggccagcgga ccac
//
LOCUS       DAOSUG010000440         2724 bp    DNA     linear   ENV 10-JUN-2024
DEFINITION  MAG TPA_asm: Micropruina sp. isolate AMR_MDS_2195
            NODE_15063_length_2724_cov_3.327838, whole genome shotgun sequence.
ACCESSION   DAOSUG010000440 DAOSUG010000000
VERSION     DAOSUG010000440.1
DBLINK      BioProject: PRJNA925477
            BioSample: SAMN32864262
            Sequence Read Archive: SRR4236673
KEYWORDS    WGS; ENV; Metagenome Assembled Genome; MAG; Third Party Data; TPA;
            TPA:assembly.
SOURCE      Micropruina sp. (wastewater metagenome)
  ORGANISM  Micropruina sp.
            Bacteria; Bacillati; Actinomycetota; Actinomycetes;
            Propionibacteriales; Nocardioidaceae; Micropruina.
REFERENCE   1  (bases 1 to 2724)
  AUTHORS   Abdulkadir,N., Saraiva,J.P., Zhang,J., Stolte,S., Gillor,O.,
            Harms,H. and Rocha,U.
  TITLE     Genome-centric analyses of 165 metagenomes show that mobile genetic
            elements are crucial for the transmission of antimicrobial
            resistance genes to pathogens in activated sludge and wastewater
  JOURNAL   Microbiol Spectr 12 (3), e0291823 (2024)
   PUBMED   38289113
REFERENCE   2  (bases 1 to 2724)
  AUTHORS   Abdulkadir,N.
  TITLE     Direct Submission
  JOURNAL   Submitted (24-MAR-2023) Environmental Microbiology, Helmholtz
            Center for Environmental Research -UFZ, Permoserstr 15, Leipzig
            04318, Germany
COMMENT     The annotation was added by the NCBI Prokaryotic Genome Annotation
            Pipeline (PGAP). Information about PGAP can be found here:
            https://www.ncbi.nlm.nih.gov/genome/annotation_prok/
            
            ##Genome-Assembly-Data-START##
            Assembly Method        :: SPAdes v. 3.11.1
            Genome Representation  :: Full
            Expected Final Version :: Yes
            Genome Coverage        :: Not Applicable
            Sequencing Technology  :: Illumina MiSeq
            ##Genome-Assembly-Data-END##
            
            ##Genome-Annotation-Data-START##
            Annotation Provider               :: NCBI
            Annotation Date                   :: 05/04/2023 04:57:10
            Annotation Pipeline               :: NCBI Prokaryotic Genome
                                                 Annotation Pipeline (PGAP)
            Annotation Method                 :: Best-placed reference protein
                                                 set; GeneMarkS-2+
            Annotation Software revision      :: 6.5
            Features Annotated                :: Gene; CDS; rRNA; tRNA; ncRNA
            Genes (total)                     :: 2,776
            CDSs (total)                      :: 2,750
            Genes (coding)                    :: 2,714
            CDSs (with protein)               :: 2,714
            Genes (RNA)                       :: 26
            tRNAs                             :: 24
            ncRNAs                            :: 2
            Pseudo Genes (total)              :: 36
            CDSs (without protein)            :: 36
            Pseudo Genes (ambiguous residues) :: 0 of 36
            Pseudo Genes (frameshifted)       :: 2 of 36
            Pseudo Genes (incomplete)         :: 32 of 36
            Pseudo Genes (internal stop)      :: 2 of 36
            ##Genome-Annotation-Data-END##
FEATURES             Location/Qualifiers
     source          1..2724
                     /organism="Micropruina sp."
                     /mol_type="genomic DNA"
                     /submitter_seqid="NODE_15063_length_2724_cov_3.327838"
                     /isolate="AMR_MDS_2195"
                     /isolation_source="Activated sludge of municipal
                     wastewater treatment plants"
                     /db_xref="taxon:2737536"
                     /environmental_sample
                     /geo_loc_name="Canada"
                     /lat_lon="49.25 N 123.1 W"
                     /collection_date="2015-06-16"
                     /metagenome_source="wastewater metagenome"
                     /note="metagenomic"
     gene            complement(<1..585)
                     /locus_tag="PLF56_12425"
     CDS             complement(<1..585)
                     /locus_tag="PLF56_12425"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_008355966.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_function: GO:0022857 - transmembrane transporter
                     activity [Evidence IEA];
                     GO_process: GO:0055085 - transmembrane transport [Evidence
                     IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="MFS transporter"
                     /protein_id="HOQ54403.1"
                     /translation="MATSVLRRLRGFLADTRPLESTAFRRLWVANIVTVIGAQLTVVA
                     VPAQIYSITGSSGWVGLTGLFGLVPLIVFGLWGGALADHFDRRTVLIITTLGLIVTSA
                     LFYLLALQAHTDVWLLLFVYAIQQACFAVNQPTRTAVLPALLPLDQLPAASSLNMTVM
                     SAGAIAGPLIGGALIPIFGFPLLYLIDTVCLLATL"
     gene            675..1250
                     /locus_tag="PLF56_12430"
     CDS             675..1250
                     /locus_tag="PLF56_12430"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_008356093.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="protein-L-isoaspartate O-methyltransferase"
                     /protein_id="HOQ54404.1"
                     /translation="MDTERRRIVAAFAATPREAFLPPQVRERASIDAPLPIGHGATNS
                     QPWTVRFMLELLQVPVGARVLDVGSGSGWTTALLARLAGPGGRVRGVEIVPELVELGC
                     LHLSGFDLPWAGIEQAEPGTYGLPAAGPFDRILVSADPGELPPQLEQQLAIGGRLVAP
                     VSGVMWVVDRDDSGWHREPVPGHRFRFVPLV"
     gene            1316..1822
                     /locus_tag="PLF56_12435"
     CDS             1316..1822
                     /locus_tag="PLF56_12435"
                     /inference="COORDINATES: ab initio
                     prediction:GeneMarkS-2+"
                     /note="Derived by automated computational analysis using
                     gene prediction method: GeneMarkS-2+."
                     /codon_start=1
                     /transl_table=11
                     /product="hypothetical protein"
                     /protein_id="HOQ54405.1"
                     /translation="MSFVVHFLPTDRAAGPHPLGDLDAAPASGASDHEEALAADEVAT
                     WDGLHPTLVTLLPPGSHDVTATMFSRQLVHESTGLMVTWAHDDYQASMPFWRENVGSG
                     ALDALAAITEAIESATGLVGVDEISEGRFLDHRDQVTATFAGVADGYEQAMEHQTVLG
                     WLRDKFRR"
     gene            complement(1829..>2724)
                     /locus_tag="PLF56_12440"
     CDS             complement(1829..>2724)
                     /locus_tag="PLF56_12440"
                     /EC_number="6.1.1.14"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_002547294.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_function: GO:0000166 - nucleotide binding [Evidence
                     IEA];
                     GO_function: GO:0004820 - glycine-tRNA ligase activity
                     [Evidence IEA];
                     GO_function: GO:0005524 - ATP binding [Evidence IEA];
                     GO_process: GO:0006426 - glycyl-tRNA aminoacylation
                     [Evidence IEA]"
                     /codon_start=3
                     /transl_table=11
                     /product="glycine--tRNA ligase"
                     /protein_id="HOQ54406.1"
                     /translation="QGIFINFKNVENSARVKVPFGIGQVGKSFRNEITPGNFIFRTRE
                     FEQMEMEFFVEPGTDEEWHQHWIDARTDWYVDLGIARDNLRLYEHPKEKLSHYSKRTV
                     DIEYDFGFAGGDGWGELEGVANRTDFDLGTHSKHSGTDLSYFDQAKNERYIPYVIEPA
                     AGLTRSLMAFLVESYTEDQAPNTKGGVDTRVVLKLDPRLSPVKVAVLPLSRNERLSPA
                     ARDLAAKLRRNWNVEFDDAQAIGRRYRRQDEIGTPYCVTVDFDTLDDQAVTVRERDTM
                     TQERVALDQVQSYLAARLIGC"
BASE COUNT          414 a          913 c          961 g          436 t
ORIGIN      
        1 caaggtggcg agcaggcaga cggtgtcgat caggtacagc agggggaacc cgaagatggg
       61 gatcagcgcg ccgccgatca gcgggccggc gatggcgccc gcgctcatca ccgtcatgtt
      121 cagcgagctg gcggcgggca gttggtccaa gggcagcagc gccggcagca ccgcggtgcg
      181 agtgggctgg ttgaccgcga agcaggcctg ttgaatggcg tagacgaaca gcagcagcca
      241 cacgtcggtg tgcgcctgca gcgccagcag gtagaacagc gcgctggtga caatgaggcc
      301 gagggtggtg atgatcagca ccgtccggcg gtcgaagtgg tcggccagcg cgccacccca
      361 caggccgaac acgatcagcg gaaccaggcc gaacaggccg gtcagcccca cccaccccga
      421 actgccggtg atgctgtaga tctgggccgg caccgccacc acggtcagct gcgcaccgat
      481 caccgtgacg atattggcca cccacaaccg gcggaacgcc gtcgactcca gtggccgggt
      541 gtcggccaga aagcctcgta gtcgtcgtag caccgaggta gccaatcacc ggatcccggt
      601 gcgggcactc cggtctcgcc gctcgaccct cgtgcggcct tgcgtgcgcg gggggatggg
      661 gcgagggttt catcgtggac accgaacgcc gacgcatcgt ggccgccttc gcggcgacgc
      721 cgcgcgaggc gttcctgccg ccgcaggtgc gcgagcgggc atcgatcgac gcaccgctgc
      781 cgatcggcca cggcgccacc aactcccagc cctggaccgt gcggttcatg ctcgaactgc
      841 tgcaggtgcc ggtgggtgcg cgggtgctcg acgtcggtag cggatcgggc tggacgaccg
      901 cgctactggc gcgactggcc gggccgggcg gccgcgtccg gggtgtcgag atcgtgcccg
      961 aactggtcga gttggggtgc ctgcacctga gcggtttcga cctgccctgg gccggcatcg
     1021 agcaggccga gccgggtacc tacgggttac cggcggcggg ccccttcgac cgcattctgg
     1081 tctcggccga cccgggcgag cttccgccgc agctcgagca gcaactggcg atcggcggac
     1141 ggctggtggc cccggtgtcc ggcgtgatgt gggtggtcga ccgcgacgac tcggggtggc
     1201 accgtgaacc ggtgccgggc catcggttcc ggttcgtgcc gctggtgtag gcccctgcgg
     1261 atcggggcgt gccgcgttgg ggaccaaccg gggcgggcgc cactacgctg acgccatgag
     1321 tttcgtcgtg cactttctac ccaccgaccg cgccgccggc ccgcacccgc tgggcgacct
     1381 cgacgctgcc ccggcgtccg gagcgtccga tcacgaagag gcgttggcag ccgacgaggt
     1441 cgcgacctgg gacggcttgc accccaccct ggtgacgctg ctgccccccg gttcgcacga
     1501 cgtgacggcc acgatgttct cacgccaact cgtgcatgag tcgaccggcc tgatggtcac
     1561 ctgggcgcac gacgactatc aggcctccat gccgttctgg cgcgagaacg tcggctccgg
     1621 ggcgctggac gccctggccg ccatcaccga ggcgatcgag tcggccaccg gcctggtcgg
     1681 tgtggacgag atcagcgagg gccgttttct cgaccaccgc gaccaggtga cggccacctt
     1741 cgcgggcgtc gccgacggct acgagcaggc catggagcat cagaccgtgc tcggctggct
     1801 gcgggacaag ttccgccgct gaggcgattc agcagccgat cagccgagcc gccaggtagg
     1861 actgcacctg atccagcgcc acccgctcct gcgtcatcgt gtcgcgctcg cgcacggtga
     1921 ccgcctggtc gtccagggtg tcgaagtcga ctgtcacgca gtagggtgtg ccgatctcgt
     1981 cctggcggcg gtagcggcgt ccgatcgcct gggcatcgtc gaactcgacg ttccagttgc
     2041 ggcgcagttt cgcggccagg tcgcgggccg ccggcgagag ccgctcgttg cgcgacagcg
     2101 gcaacaccgc caccttgacc ggcgacagcc gcggatcgag cttgagcacc acccgggtgt
     2161 cgacgccgcc cttggtgttc ggcgcctgat cctcggtgta ggactcgacc aggaacgcca
     2221 tcagcgaccg ggtcaggccg gccgccggct cgatcacgta cggaatgtag cgctcgttct
     2281 tggcctgatc gaagtaggag aggtcggtgc ccgagtgctt cgaatgggtg ccgagatcga
     2341 agtcggtgcg gttcgcgacg ccttcgagct cgccccagcc gtcgccgccg gcgaagccga
     2401 agtcgtactc gatgtcgacg gtgcgcttgc tgtagtgcga cagcttctct ttcgggtgct
     2461 cgtagagccg cagattgtct cgcgcaatgc ccaggtcgac gtaccagtcg gtgcgggcgt
     2521 cgatccagtg ctggtgccac tcctcgtcgg tgcccggctc gacgaagaac tccatctcca
     2581 tctgctcgaa ctcacgggtg cggaagatga agttgcccgg ggtgatctcg ttgcggaagc
     2641 tcttgccgac ctggccgatg ccgaacggca ccttcacccg cgccgagttc tcgacgttct
     2701 tgaagttgat gaagatgccc tgcg
//
LOCUS       DAOSUG010000441         2716 bp    DNA     linear   ENV 10-JUN-2024
DEFINITION  MAG TPA_asm: Micropruina sp. isolate AMR_MDS_2195
            NODE_15112_length_2716_cov_4.316422, whole genome shotgun sequence.
ACCESSION   DAOSUG010000441 DAOSUG010000000
VERSION     DAOSUG010000441.1
DBLINK      BioProject: PRJNA925477
            BioSample: SAMN32864262
            Sequence Read Archive: SRR4236673
KEYWORDS    WGS; ENV; Metagenome Assembled Genome; MAG; Third Party Data; TPA;
            TPA:assembly.
SOURCE      Micropruina sp. (wastewater metagenome)
  ORGANISM  Micropruina sp.
            Bacteria; Bacillati; Actinomycetota; Actinomycetes;
            Propionibacteriales; Nocardioidaceae; Micropruina.
REFERENCE   1  (bases 1 to 2716)
  AUTHORS   Abdulkadir,N., Saraiva,J.P., Zhang,J., Stolte,S., Gillor,O.,
            Harms,H. and Rocha,U.
  TITLE     Genome-centric analyses of 165 metagenomes show that mobile genetic
            elements are crucial for the transmission of antimicrobial
            resistance genes to pathogens in activated sludge and wastewater
  JOURNAL   Microbiol Spectr 12 (3), e0291823 (2024)
   PUBMED   38289113
REFERENCE   2  (bases 1 to 2716)
  AUTHORS   Abdulkadir,N.
  TITLE     Direct Submission
  JOURNAL   Submitted (24-MAR-2023) Environmental Microbiology, Helmholtz
            Center for Environmental Research -UFZ, Permoserstr 15, Leipzig
            04318, Germany
COMMENT     The annotation was added by the NCBI Prokaryotic Genome Annotation
            Pipeline (PGAP). Information about PGAP can be found here:
            https://www.ncbi.nlm.nih.gov/genome/annotation_prok/
            
            ##Genome-Assembly-Data-START##
            Assembly Method        :: SPAdes v. 3.11.1
            Genome Representation  :: Full
            Expected Final Version :: Yes
            Genome Coverage        :: Not Applicable
            Sequencing Technology  :: Illumina MiSeq
            ##Genome-Assembly-Data-END##
            
            ##Genome-Annotation-Data-START##
            Annotation Provider               :: NCBI
            Annotation Date                   :: 05/04/2023 04:57:10
            Annotation Pipeline               :: NCBI Prokaryotic Genome
                                                 Annotation Pipeline (PGAP)
            Annotation Method                 :: Best-placed reference protein
                                                 set; GeneMarkS-2+
            Annotation Software revision      :: 6.5
            Features Annotated                :: Gene; CDS; rRNA; tRNA; ncRNA
            Genes (total)                     :: 2,776
            CDSs (total)                      :: 2,750
            Genes (coding)                    :: 2,714
            CDSs (with protein)               :: 2,714
            Genes (RNA)                       :: 26
            tRNAs                             :: 24
            ncRNAs                            :: 2
            Pseudo Genes (total)              :: 36
            CDSs (without protein)            :: 36
            Pseudo Genes (ambiguous residues) :: 0 of 36
            Pseudo Genes (frameshifted)       :: 2 of 36
            Pseudo Genes (incomplete)         :: 32 of 36
            Pseudo Genes (internal stop)      :: 2 of 36
            ##Genome-Annotation-Data-END##
FEATURES             Location/Qualifiers
     source          1..2716
                     /organism="Micropruina sp."
                     /mol_type="genomic DNA"
                     /submitter_seqid="NODE_15112_length_2716_cov_4.316422"
                     /isolate="AMR_MDS_2195"
                     /isolation_source="Activated sludge of municipal
                     wastewater treatment plants"
                     /db_xref="taxon:2737536"
                     /environmental_sample
                     /geo_loc_name="Canada"
                     /lat_lon="49.25 N 123.1 W"
                     /collection_date="2015-06-16"
                     /metagenome_source="wastewater metagenome"
                     /note="metagenomic"
     gene            159..755
                     /locus_tag="PLF56_12445"
     CDS             159..755
                     /locus_tag="PLF56_12445"
                     /inference="COORDINATES: protein motif:HMM:NF012464.2"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_function: GO:0000150 - DNA strand exchange activity
                     [Evidence IEA];
                     GO_function: GO:0003677 - DNA binding [Evidence IEA];
                     GO_process: GO:0006310 - DNA recombination [Evidence IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="recombinase family protein"
                     /protein_id="HOQ54407.1"
                     /translation="MRQLGYTRVSTVTQDAQLQLDALVGAGVQKRDVFADVTSGSRAA
                     AERPGMKKLLAYVQPGDTVVVWRVDRLGRSLIDVLKTVNLLRDRDVHVRSVADGIDPA
                     TSTGRLMLNMLATLAEYERELIVERVTAGIVAARHRGTQFGRPPSDPRVVADKLAFAA
                     DARAKGRTAEEAARLVGWSRATLYRHQQAQAAREDNGM"
     gene            764..1165
                     /locus_tag="PLF56_12450"
     CDS             764..1165
                     /locus_tag="PLF56_12450"
                     /inference="COORDINATES: protein motif:HMM:NF024954.2"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="helix-turn-helix domain-containing protein"
                     /protein_id="HOQ54408.1"
                     /translation="MDADGKWRVLRLHVEDQIPLAALARDTGIGLRTLERWHHRYRNG
                     GISALEQQPRTDSGHKRTKGALVAFVEHLALTRPRPSIATLHRLAVAEAERQELVPPS
                     YSTVRNIVQALDPALASHPGVGGCCGIPGPV"
     gene            1131..2159
                     /locus_tag="PLF56_12455"
     CDS             1131..2159
                     /locus_tag="PLF56_12455"
                     /inference="COORDINATES: protein motif:HMM:NF012870.2"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="Mu transposase C-terminal domain-containing
                     protein"
                     /protein_id="HOQ54409.1"
                     /translation="MEGAAAYRDRYELVHRFRADRPNQLWQADHTELDILTVGPNGKP
                     DRPWLTAVMDDHSRAICGYTVFTGAPSALQTALALRQAIWRKSDPSWAMSGLPDVLYV
                     DHGSDFTSHHLERTAIELHIRIIHSTIARPQGRGKIERFFGTINTELLATLPGHLGPG
                     SHTPTPVLDLSELDHAVGGFVATYNDRSHQEIGSSPRDAWVADGWLPRLPDSLEQLDG
                     LLLTVPKHRTVHRDGIHFQGHRYLSGTLAPFVGHPVTIRYDPRDLSEIRVYDQSTFLC
                     VAIDEDHPNQRLSLRDIEATRRDRRRQLRRSLNDRIPTLAHRDEPDTTPAPAPRRRRL
                     RTYEEDDG"
     gene            2156..>2716
                     /locus_tag="PLF56_12460"
     CDS             2156..>2716
                     /locus_tag="PLF56_12460"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_010613290.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="AAA family ATPase"
                     /protein_id="HOQ54410.1"
                     /translation="MIQTFIVTKEHRRFTEFANAVRAERTIGICHGDAGVGKTNSARR
                     YANWDTLEPYLDTWGPRSDDDAKHYALANRARTVVYTPEVLPRHRELVRDIDHWVSRL
                     GICIEEHQRTIGKVTGTHVGDASRWVELLIIDEAERLTPTALEMLRDRHDRTQLAIIL
                     IGMPGIDQRFRHYPQLYSRLGFSHRYR"
BASE COUNT          530 a         1012 c          763 g          411 t
ORIGIN      
        1 aggcggccgt gcagaagacc tcggacacct gtgcaggcga ctgcggactt cgacacccat
       61 cgctcggatc gaggcggtgg accagtctca gaaacgtgtc tcgacggcaa tcgtcttaac
      121 tcatccccct ttgagggttt ttgaggcaca cttgctgcgt gaggcagctc gggtacacga
      181 gggtgagcac cgtcacacag gatgcgcagc tgcagctcga cgcactggtt ggtgctgggg
      241 tgcagaagcg tgacgtgttc gctgatgtca cctcgggcag tagggcagcg gctgagcgcc
      301 ctggcatgaa gaagctgctc gcctacgtcc agcccggcga cacggtggtg gtgtggcgcg
      361 ttgaccgtct cggtcggtct ctgattgatg tcctcaaaac ggtgaacctg ctccgtgacc
      421 gcgacgttca cgttcggtcg gtggctgacg ggatcgaccc tgccacctca acgggtcggc
      481 tgatgttgaa catgcttgcc accctcgcag agtacgagcg ggaactgatc gtcgagcggg
      541 tcaccgccgg catcgtcgcc gcccgacaca ggggcaccca gttcggccgt ccgccttcag
      601 acccgagggt ggttgccgac aagctcgcct tcgccgccga tgcccgggct aaaggccgga
      661 cagccgaaga agccgcccgg ctcgtcgggt ggagccgcgc caccttgtac cgccaccaac
      721 aggcacaggc agcccgcgag gacaacggaa tgtagctgga ccggtggacg ctgacggaaa
      781 gtggcgggtc cttcgactcc atgtcgaaga ccagatcccg ctcgcggcgc tggcccgcga
      841 caccggcatc ggcctccgca ccctggaacg ttggcatcat cgttaccgga atgggggcat
      901 cagcgcgctc gaacaacaac ctcgaaccga ctcaggacac aagcgcacga agggcgcttt
      961 ggtggccttc gtcgaacatc tcgcgctgac ccggccccga cccagcatcg cgactctgca
     1021 ccgcctggct gtcgccgaag cggaacggca ggaactagtg ccacccagtt actccacggt
     1081 gcgcaacatc gtgcaagccc tggacccggc gctagctagt caccctggcg ttggagggtg
     1141 ctgcggcata ccgggaccgg tatgaactcg tccaccggtt ccgtgccgac cgcccgaacc
     1201 agctgtggca ggctgaccac accgaactcg acatacttac tgtcggcccg aacggtaaac
     1261 ctgaccggcc atggctgacc gcggtgatgg atgaccactc ccgagcaatc tgtggctaca
     1321 ccgtcttcac cggcgcccca tccgcactgc aaaccgcgct tgccttacgc caagccatct
     1381 ggcgcaagag tgacccgagc tgggcgatga gcggccttcc cgacgtgttg tatgtcgacc
     1441 acggctccga cttcaccagc catcacctcg agcgcaccgc aattgagctg cacatccgga
     1501 tcatccactc caccatcgcc cgcccccaag gccgcgggaa gatcgagcga ttcttcggca
     1561 ctatcaacac cgaactcctc gccacactgc ccggtcacct cggaccaggc agccacacac
     1621 cgacacccgt gctcgacctg tcggaactcg accacgcagt gggcggcttc gttgctacct
     1681 acaacgaccg atcccatcag gagatcggca gttcaccgcg cgacgcctgg gtcgcagacg
     1741 gatggctacc ccgtctgcct gacagtctcg aacaactcga cgggctactc ctcaccgtcc
     1801 ccaagcaccg caccgtgcat cgcgacggca tccacttcca aggccaccgc tacctctccg
     1861 gaacgctggc cccattcgtc gggcacccag tgaccatccg atacgacccg cgggacctct
     1921 cagagatccg cgtctacgac cagagcacct tcctgtgcgt cgccatcgac gaggaccacc
     1981 ccaaccagcg actcagcctt cgcgacattg aagcgacccg acgggaccgc cgccgccaac
     2041 tccgccgcag cctcaacgac cgcatcccca ccctcgccca ccgcgacgag ccggacacga
     2101 ctccagcccc tgcaccccgc cggcggcgac tccgaaccta cgaagaggac gacggatgat
     2161 ccaaaccttc atcgtgacca aggaacaccg ccgcttcacc gagttcgcca acgccgtgcg
     2221 cgccgagcgc accatcggca tctgccacgg cgacgccggc gtcgggaaga ccaactccgc
     2281 ccgccgctac gccaactggg acaccttgga gccctacctc gacacctggg gaccacgcag
     2341 cgacgacgac gccaagcact acgctctcgc caatcgtgcc cgcaccgtcg tctacacccc
     2401 ggaagtgctg ccccggcacc gcgaactggt ccgcgacatc gaccactggg tcagccgcct
     2461 cggcatttgt atcgaggagc accaacgcac catcggcaaa gtcaccggca cccacgtcgg
     2521 cgacgcgagc cgctgggtcg agctcctcat catcgacgaa gccgaacgac tcacacccac
     2581 cgccctggaa atgctccgcg accgacacga ccgcacccag ctcgccatca tcctgatcgg
     2641 catgcccggc atcgaccaac ggttccggca ctacccccag ctctacagcc gcctcggctt
     2701 ctcccaccgc taccgc
//
LOCUS       DAOSUG010000442         2715 bp    DNA     linear   ENV 10-JUN-2024
DEFINITION  MAG TPA_asm: Micropruina sp. isolate AMR_MDS_2195
            NODE_15119_length_2715_cov_3.720301, whole genome shotgun sequence.
ACCESSION   DAOSUG010000442 DAOSUG010000000
VERSION     DAOSUG010000442.1
DBLINK      BioProject: PRJNA925477
            BioSample: SAMN32864262
            Sequence Read Archive: SRR4236673
KEYWORDS    WGS; ENV; Metagenome Assembled Genome; MAG; Third Party Data; TPA;
            TPA:assembly.
SOURCE      Micropruina sp. (wastewater metagenome)
  ORGANISM  Micropruina sp.
            Bacteria; Bacillati; Actinomycetota; Actinomycetes;
            Propionibacteriales; Nocardioidaceae; Micropruina.
REFERENCE   1  (bases 1 to 2715)
  AUTHORS   Abdulkadir,N., Saraiva,J.P., Zhang,J., Stolte,S., Gillor,O.,
            Harms,H. and Rocha,U.
  TITLE     Genome-centric analyses of 165 metagenomes show that mobile genetic
            elements are crucial for the transmission of antimicrobial
            resistance genes to pathogens in activated sludge and wastewater
  JOURNAL   Microbiol Spectr 12 (3), e0291823 (2024)
   PUBMED   38289113
REFERENCE   2  (bases 1 to 2715)
  AUTHORS   Abdulkadir,N.
  TITLE     Direct Submission
  JOURNAL   Submitted (24-MAR-2023) Environmental Microbiology, Helmholtz
            Center for Environmental Research -UFZ, Permoserstr 15, Leipzig
            04318, Germany
COMMENT     The annotation was added by the NCBI Prokaryotic Genome Annotation
            Pipeline (PGAP). Information about PGAP can be found here:
            https://www.ncbi.nlm.nih.gov/genome/annotation_prok/
            
            ##Genome-Assembly-Data-START##
            Assembly Method        :: SPAdes v. 3.11.1
            Genome Representation  :: Full
            Expected Final Version :: Yes
            Genome Coverage        :: Not Applicable
            Sequencing Technology  :: Illumina MiSeq
            ##Genome-Assembly-Data-END##
            
            ##Genome-Annotation-Data-START##
            Annotation Provider               :: NCBI
            Annotation Date                   :: 05/04/2023 04:57:10
            Annotation Pipeline               :: NCBI Prokaryotic Genome
                                                 Annotation Pipeline (PGAP)
            Annotation Method                 :: Best-placed reference protein
                                                 set; GeneMarkS-2+
            Annotation Software revision      :: 6.5
            Features Annotated                :: Gene; CDS; rRNA; tRNA; ncRNA
            Genes (total)                     :: 2,776
            CDSs (total)                      :: 2,750
            Genes (coding)                    :: 2,714
            CDSs (with protein)               :: 2,714
            Genes (RNA)                       :: 26
            tRNAs                             :: 24
            ncRNAs                            :: 2
            Pseudo Genes (total)              :: 36
            CDSs (without protein)            :: 36
            Pseudo Genes (ambiguous residues) :: 0 of 36
            Pseudo Genes (frameshifted)       :: 2 of 36
            Pseudo Genes (incomplete)         :: 32 of 36
            Pseudo Genes (internal stop)      :: 2 of 36
            ##Genome-Annotation-Data-END##
FEATURES             Location/Qualifiers
     source          1..2715
                     /organism="Micropruina sp."
                     /mol_type="genomic DNA"
                     /submitter_seqid="NODE_15119_length_2715_cov_3.720301"
                     /isolate="AMR_MDS_2195"
                     /isolation_source="Activated sludge of municipal
                     wastewater treatment plants"
                     /db_xref="taxon:2737536"
                     /environmental_sample
                     /geo_loc_name="Canada"
                     /lat_lon="49.25 N 123.1 W"
                     /collection_date="2015-06-16"
                     /metagenome_source="wastewater metagenome"
                     /note="metagenomic"
     gene            <1..1973
                     /locus_tag="PLF56_12465"
     CDS             <1..1973
                     /locus_tag="PLF56_12465"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_002547726.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_function: GO:0005524 - ATP binding [Evidence IEA];
                     GO_function: GO:0016787 - hydrolase activity [Evidence
                     IEA]"
                     /codon_start=3
                     /transl_table=11
                     /product="3'-5' exonuclease"
                     /protein_id="HOQ54411.1"
                     /translation="SIYDDTDAKRLMTLVLRDQEVDPKRYPVRTVMNWVSNCKNELVD
                     HETAAARAETPNEKVYAEAYRTYQSRLASANALDFDDLIMTTVHLLQAFPDLREQYRR
                     RFRHVLVDEYQDTNHAQYALVRELCADLPVPEGMTAVEPPELMVVGDSDQSIYAFRGA
                     TIRNILAFEADFPGATTIVLEQNYRSTQTILNAANAVITRNPGRPQKRLWSEAGDGER
                     IVGWVADTEHDEAQFVADEIDRLSDAGHAKPGDVAVFYRTNAQSRAFEEVFIRVGMPY
                     RVVGGVRFYERREVRDAIAYLRSIANPADDVSVRRILNVPKRGIGDRAEAAVEALAAA
                     ERIPFGMALDRADEASGLATRSLTQLQGFAALMAEHRAMMARGEPADAILVSILEKSG
                     YIDELKNSDDPQDATRLENLIELVSVAQEFVAAAHAIDLPAEGGELAAGAPEPDDSLP
                     AFLERIALVADSDQIPDADGEGTGVVTLMTLHTAKGLEFPVVFLTGFEDGIFPHQRAL
                     SDNDELQEERRLAYVGITRARQRLYLSRAVVRTLWGTPQYNPPSRFTQEIPEHLFDWR
                     RLQAASVGFTSRAKARTEQAWRNTVGYGSAPSLPKAVPSVQPGDRVLHTVFGMGTVVA
                     TSGMGDLAKADVDFGSTGVKRLSLKHAPMEKL"
     gene            complement(2007..>2715)
                     /locus_tag="PLF56_12470"
     CDS             complement(2007..>2715)
                     /locus_tag="PLF56_12470"
                     /inference="COORDINATES: protein motif:HMM:NF013700.2"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=2
                     /transl_table=11
                     /product="peptidoglycan DD-metalloendopeptidase family
                     protein"
                     /protein_id="HOQ54412.1"
                     /translation="LAVGLAGQATRSSSAAPVPQIGVGRLDLGALNDRDNGVSRDSAR
                     VATPQVIAQSRANALAQTAKELAAQRQANALEERTESLDATVDQIEQQSDRLNDASSF
                     LMPTAGKFGSGFGMRLHPILHYWRMHNGQDIGGTCGQPIWAAQDGTVIKVAPNGYNGG
                     SGHNVRIDGGEVGGVNLQTAYLHMDTIAVQVGQKVHKGELLGTVGNTGISTACHLHFS
                     VYVDGKGVDPVPYLKNS"
BASE COUNT          442 a          898 c          954 g          421 t
ORIGIN      
        1 tctcgatcta cgacgacacc gacgccaagc ggctgatgac cctggtgttg cgcgatcagg
       61 aggtcgaccc gaagcggtac cccgtccgca cggtgatgaa ctgggtgagc aactgcaaga
      121 acgagcttgt cgaccatgaa accgccgcgg cccgggccga aacgcccaac gagaaggtgt
      181 acgccgaggc gtaccgcacc taccagagcc ggctggcctc ggccaacgcg ctcgacttcg
      241 acgacctgat catgaccacg gtgcacctgc tgcaggcctt tcccgacctg cgcgagcagt
      301 accggcggcg ctttcggcac gtgctcgtcg atgagtacca ggacaccaac cacgcccagt
      361 acgccctggt gcgtgagttg tgcgccgact tgcccgtgcc cgagggcatg acggcggtcg
      421 agccgcccga gttgatggtg gtgggcgact ccgaccagtc gatctatgcg ttccggggcg
      481 cgacgatccg caacatcctg gccttcgagg ccgactttcc gggtgcaacc accattgtgc
      541 tcgagcagaa ctaccgctcg acgcagacga ttctgaacgc cgcgaacgca gtgatcacgc
      601 gtaacccggg ccgtccgcag aaacgattgt ggtccgaggc gggcgacggc gagcgcatcg
      661 tcggctgggt cgccgacacc gagcacgacg aggcccagtt cgtggctgat gagatcgacc
      721 ggctgagtga cgcagggcac gccaagccgg gcgacgtggc cgtcttctac cgcaccaacg
      781 cccagtcgcg agccttcgaa gaggtgttca tccgggtcgg catgccctac cgggtggtgg
      841 gcggcgtccg cttctacgag cggcgtgagg tgcgggacgc gatcgcctac ctgcgcagca
      901 tcgccaaccc ggcggacgac gtctcggtgc ggcgcatcct caacgtgccc aagcggggga
      961 ttggtgaccg cgccgaggcg gcggtcgagg cgctggcggc ggcggaacgc attccgttcg
     1021 gaatggcgct cgaccgggcg gacgaggcgt ccgggctggc gacccgctcg ctcacccaac
     1081 tgcaggggtt cgccgcactg atggccgagc accgcgcgat gatggcgcgc ggcgagccgg
     1141 cggacgcgat cctggtcagc attctggaga agtcgggcta catcgacgag ttgaagaact
     1201 ccgacgatcc gcaggacgcc acccggctcg agaacctgat cgagttggtg tcggtggcgc
     1261 aggagttcgt cgcggcggcc cacgcgatcg acctgcccgc cgagggcggc gagctggccg
     1321 caggcgcccc cgaacccgac gacagcctgc cggcgtttct ggagcgcatc gcgctggtgg
     1381 ccgattccga ccagattccg gacgccgacg gcgagggcac cggggtggtc accctgatga
     1441 ccctgcacac cgcgaagggc ctggagtttc cggtggtgtt cctgaccggt ttcgaggacg
     1501 gcatctttcc ccaccagcgc gcactgagcg acaacgacga actgcaggag gagcggcggc
     1561 tcgcctacgt gggcatcacc cgggcccggc agcggctgta cctgagccgc gccgtcgtcc
     1621 ggacgttgtg gggcacgccg cagtacaacc cgcccagccg tttcacgcag gagattcccg
     1681 agcacctgtt cgactggagg cggctgcagg cggcgtcggt gggattcacc tcacgcgcca
     1741 aggcgcgcac cgaacaggcc tggcgcaaca cggtgggcta cggatcggca ccctcgctgc
     1801 ccaaggcggt gccgtcggtg cagccgggcg accgggtgct gcacaccgtg ttcggcatgg
     1861 gcacggtggt ggcgaccagc ggtatgggcg atctggcgaa ggcggacgtc gacttcggca
     1921 gcaccggggt gaagcggctc agcctcaagc acgctccgat ggagaagctg tagaagatcc
     1981 gggcccaggc ttaggtgagc gccggttcag gagttcttca ggtacggcac cgggtcgacg
     2041 cccttgccgt cgacgtacac cgagaaatgc aggtggcagg ctgtcgaaat gccggtgttg
     2101 ccgacggtgc cgagcaactc acccttgtgc accttctggc ccacctgcac cgcgatcgtg
     2161 tccatgtgca ggtaggcggt ctgcaggttg acgccgccca cctcgccgcc gtcgatgcgc
     2221 acgttgtggc ccgagccgcc gttgtagcca ttgggggcga ccttgatcac ggtgccgtcc
     2281 tgggcggccc agatcggttg accgcaggtg ccgccgatgt cctggccgtt gtgcatgcgc
     2341 cagtagtgca ggatcgggtg caaccgcatg ccgaacccgg agccgaactt gccggccgtc
     2401 ggcatcagaa agctggacgc gtcgttcagc cgatcggact gctgctcgat ctggtcgacg
     2461 gtcgcatcga gtgactcggt gcgctcttcg agcgcattgg cctgccgttg ggcagcgagc
     2521 tccttggcgg tctgggccag cgcgttcgcc cggctctggg cgatgacctg cggcgttgcg
     2581 acccgggcgg aatcacggct gaccccgttg tcgcggtcgt tcagcgcgcc caggtcgagc
     2641 cggcccacgc cgatctgggg taccggtgcg gcggacgacg agcgggtcgc ctggccggcc
     2701 aagccgaccg cgaga
//
LOCUS       DAOSUG010000443         2714 bp    DNA     linear   ENV 10-JUN-2024
DEFINITION  MAG TPA_asm: Micropruina sp. isolate AMR_MDS_2195
            NODE_15129_length_2714_cov_3.703272, whole genome shotgun sequence.
ACCESSION   DAOSUG010000443 DAOSUG010000000
VERSION     DAOSUG010000443.1
DBLINK      BioProject: PRJNA925477
            BioSample: SAMN32864262
            Sequence Read Archive: SRR4236673
KEYWORDS    WGS; ENV; Metagenome Assembled Genome; MAG; Third Party Data; TPA;
            TPA:assembly.
SOURCE      Micropruina sp. (wastewater metagenome)
  ORGANISM  Micropruina sp.
            Bacteria; Bacillati; Actinomycetota; Actinomycetes;
            Propionibacteriales; Nocardioidaceae; Micropruina.
REFERENCE   1  (bases 1 to 2714)
  AUTHORS   Abdulkadir,N., Saraiva,J.P., Zhang,J., Stolte,S., Gillor,O.,
            Harms,H. and Rocha,U.
  TITLE     Genome-centric analyses of 165 metagenomes show that mobile genetic
            elements are crucial for the transmission of antimicrobial
            resistance genes to pathogens in activated sludge and wastewater
  JOURNAL   Microbiol Spectr 12 (3), e0291823 (2024)
   PUBMED   38289113
REFERENCE   2  (bases 1 to 2714)
  AUTHORS   Abdulkadir,N.
  TITLE     Direct Submission
  JOURNAL   Submitted (24-MAR-2023) Environmental Microbiology, Helmholtz
            Center for Environmental Research -UFZ, Permoserstr 15, Leipzig
            04318, Germany
COMMENT     The annotation was added by the NCBI Prokaryotic Genome Annotation
            Pipeline (PGAP). Information about PGAP can be found here:
            https://www.ncbi.nlm.nih.gov/genome/annotation_prok/
            
            ##Genome-Assembly-Data-START##
            Assembly Method        :: SPAdes v. 3.11.1
            Genome Representation  :: Full
            Expected Final Version :: Yes
            Genome Coverage        :: Not Applicable
            Sequencing Technology  :: Illumina MiSeq
            ##Genome-Assembly-Data-END##
            
            ##Genome-Annotation-Data-START##
            Annotation Provider               :: NCBI
            Annotation Date                   :: 05/04/2023 04:57:10
            Annotation Pipeline               :: NCBI Prokaryotic Genome
                                                 Annotation Pipeline (PGAP)
            Annotation Method                 :: Best-placed reference protein
                                                 set; GeneMarkS-2+
            Annotation Software revision      :: 6.5
            Features Annotated                :: Gene; CDS; rRNA; tRNA; ncRNA
            Genes (total)                     :: 2,776
            CDSs (total)                      :: 2,750
            Genes (coding)                    :: 2,714
            CDSs (with protein)               :: 2,714
            Genes (RNA)                       :: 26
            tRNAs                             :: 24
            ncRNAs                            :: 2
            Pseudo Genes (total)              :: 36
            CDSs (without protein)            :: 36
            Pseudo Genes (ambiguous residues) :: 0 of 36
            Pseudo Genes (frameshifted)       :: 2 of 36
            Pseudo Genes (incomplete)         :: 32 of 36
            Pseudo Genes (internal stop)      :: 2 of 36
            ##Genome-Annotation-Data-END##
FEATURES             Location/Qualifiers
     source          1..2714
                     /organism="Micropruina sp."
                     /mol_type="genomic DNA"
                     /submitter_seqid="NODE_15129_length_2714_cov_3.703272"
                     /isolate="AMR_MDS_2195"
                     /isolation_source="Activated sludge of municipal
                     wastewater treatment plants"
                     /db_xref="taxon:2737536"
                     /environmental_sample
                     /geo_loc_name="Canada"
                     /lat_lon="49.25 N 123.1 W"
                     /collection_date="2015-06-16"
                     /metagenome_source="wastewater metagenome"
                     /note="metagenomic"
     gene            complement(36..689)
                     /locus_tag="PLF56_12475"
     CDS             complement(36..689)
                     /locus_tag="PLF56_12475"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_013862388.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="uridine kinase"
                     /protein_id="HOQ54413.1"
                     /translation="MQNKPGEPLSRQAGLAWLVSRIRPRAGRTIVAIDGVDGAGKTTF
                     AGELADELTDSGITVIRISLDDFLNPPEIRHQQGRSSPEGFVADTYDYERFIDDVLEP
                     LSGEGCGRYRPKSYDYPTETPLTPPWQVAPDQAVVIVEGMFLHRNRLRNARGRKVWDL
                     SVWLDVPFEVSVKRLAERDGSPDDPSDERIARYVQGQRLYIQLANPAERADVVISNS"
     gene            complement(670..2100)
                     /locus_tag="PLF56_12480"
     CDS             complement(670..2100)
                     /locus_tag="PLF56_12480"
                     /EC_number="3.4.-.-"
                     /inference="COORDINATES: protein motif:HMM:NF014566.2"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_component: GO:0016020 - membrane [Evidence IEA];
                     GO_function: GO:0004222 - metalloendopeptidase activity
                     [Evidence IEA];
                     GO_process: GO:0071586 - CAAX-box protein processing
                     [Evidence IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="CPBP family intramembrane metalloprotease"
                     /protein_id="HOQ54414.1"
                     /translation="MSLTPDGPHSDGDAPVQDPSATPAYSAGPSPSAQPGEPAQSGGS
                     GPGGQPGQYPGQPSAQVYPAQPSPQVYPGQPPYPGQPYQGQPSPQAYPVQPSPQVYPG
                     QPPYPGQQPYPPQPGGWPQPGAAPVPGPPAGHPWLPQQPVAKPSVLPVEPREYHEFLR
                     TPAMRWWRPIAALAMGAAIWFVVNVVAGGLAMIYDLAVGNTTWADYGSLDGFKTTPAF
                     FLANNLALASAIPIAALTQWACFGQRPRWLPSVVGEFRWRWCLECVAWLLPLYLLSLL
                     IDLVLWGPPTIQVTAGTGFMIAAILLTTPLQAAGEEYLLRGLGQRAIGAWLPRTAGLV
                     ISTAVTAVIFMLLHGAGDPWLNAFYLLFAVTASVLVWRTGGLEAAIAMHAVNNVVSMT
                     FLPFTDISDMFDREAGVGSPWILVQMAIMLGGAALVLWRAKRRNVVTMAAPGAPQAAP
                     TWQSHHPPQADPPQQPWRRNDAEQTR"
     gene            complement(2097..2507)
                     /gene="ndk"
                     /locus_tag="PLF56_12485"
     CDS             complement(2097..2507)
                     /gene="ndk"
                     /locus_tag="PLF56_12485"
                     /EC_number="2.7.4.6"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_020577361.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_function: GO:0004550 - nucleoside diphosphate kinase
                     activity [Evidence IEA];
                     GO_process: GO:0006165 - nucleoside diphosphate
                     phosphorylation [Evidence IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="nucleoside-diphosphate kinase"
                     /protein_id="HOQ54415.1"
                     /translation="MSDRTFVIIKPDAVRRGLVGAILARYESKGLRVAALQQRTIDAD
                     LADRHYAEHTAQPWYPPLREFITSGPLVALILEGPSAIEAVRALNGATNGVAAAPGSI
                     RGDYSLSNRENAVHASDSPESAQREIALWFPESA"
BASE COUNT          434 a          935 c          971 g          374 t
ORIGIN      
        1 cggtggctag tggcgtgtcc cctgcgccac cccgctcagc tgttgctgat caccacgtcg
       61 gcgcgctcgg cggggttggc gagctgaatg tagagccgct ggccctgcac gtagcgggcg
      121 atgcgctcat cggaggggtc gtcgggcgaa ccgtcgcgct cggcgagccg cttgaccgat
      181 acctcgaatg gcacgtcgag ccacaccgac aggtcccaca ccttgcggcc gcgggcgttg
      241 cgcagccggt tgcggtgcag gaacatgccc tcgacgatca ccaccgcctg gtcgggggcc
      301 acctgccacg gcggggtgag cggggtttcg gttgggtagt cgtacgactt gggacggtag
      361 cgtccacaac cctcgccgct caacggctcg agcacgtcgt cgatgaaccg ctcgtagtcg
      421 taggtgtcgg cgacgaagcc ttcgggcgag gagcgaccct gctggtggcg aatctcgggc
      481 gggttcagaa aatcgtccag cgagatccgg atcacggtga tgcccgagtc ggtcagttcg
      541 tcggccaact ccccggcgaa ggtggtcttg ccggcgccgt ccacgccgtc gatggcgacg
      601 atggtgcgcc ctgctcgtgg acggatgcgc gagaccagcc aggccaggcc ggcctgacgg
      661 ctcagcggct caccgggttt gttctgcatc gtttcgcctc cagggctgct gcggtgggtc
      721 tgcctgcggc gggtggtgcg actgccacgt cggtgccgcc tgcggcgcac cgggcgccgc
      781 catcgtaacc acgttgcgcc gcttcgcgcg ccacagcacg agtgcagctc cccccagcat
      841 gatcgccatc tgcaccagaa tccaggggct gccgacaccg gcctcgcggt cgaacatgtc
      901 cgagatgtcg gtgaacggca gaaaggtcat cgagaccaca ttgttcacag cgtgcatggc
      961 gatggccgcc tcgagcccac ccgtgcgcca caccagcacc gaggcggtga cggcgaacag
     1021 caggtagaag gcgttcagcc aggggtcacc ggcgccatgc agcagcatga agatcaccgc
     1081 tgtcaccgcc gtgctgatca ccaggccggc ggtgcggggc aaccaggcgc cgatcgcacg
     1141 ctggcccaga ccccgcagca ggtactcctc cccggccgcc tgcaacggcg tggtgagcag
     1201 gatcgccgcg atcatgaagc ccgtgccggc tgtgacctgg atcgtgggcg gaccccacag
     1261 caccaggtcg atcagcaggc tgagcagata cagcggcagc agccaggcca cgcactccaa
     1321 gcaccagcgc caccggaact cacccaccac ggacggcagc cagcgcggcc gctgcccgaa
     1381 acaggcccac tgggtgagcg ccgcgatcgg gatcgccgag gcgagggcca ggttattggc
     1441 cagaaagaag gccggagtgg tcttgaaccc gtccaggctg ccgtagtcgg cccaggtggt
     1501 gttgccgacg gccaggtcgt agatcatcgc cagcccgccg gccacaacgt tcacgacgaa
     1561 ccagatcgcc gcgcccatgg cgagcgccgc gatcggacgc caccagcgca tggccggcgt
     1621 gcgcagaaac tcgtgatact cccgcggctc gaccggcagt acggacggct tcgcgaccgg
     1681 ttgctgcggc agccacggat gtccggcggg cgggccgggc accggggcgg caccgggctg
     1741 cggccagccg cccggctgcg gcgggtaggg ctgttggccc gggtagggcg gctggcccgg
     1801 gtacacctgc ggggacggct gaaccgggta cgcctgcggg gacggctggc cctggtacgg
     1861 ctggcccggg tagggcggct ggcccgggta cacctgcggg gacggctgag ccgggtacac
     1921 ctgcgcggac ggctggcccg ggtactgtcc gggttgcccg cccggccccg agcccccgga
     1981 ctgggcgggc tcaccgggct gtgccgaggg agacggaccg gccgagtagg ccggcgtggc
     2041 ggatgggtcc tgaaccggcg cgtcaccgtc gctgtgcggg ccgtcggggg tgaggctcac
     2101 gccgactccg gaaaccacag ggcgatctcg cgctgagccg attcgggcga atcggaggca
     2161 tgcaccgcgt tctcgcggtt cgacagcgag tagtcgcccc ggatcgatcc cggcgcggcg
     2221 gccaccccgt tggtggcacc gttcagcgcg cgaaccgcct cgatcgccga ggggccctcg
     2281 agaatcagcg cgaccaatgg ccctgaggtg atgaactcgc gcaacggcgg gtaccacggc
     2341 tgcgcggtgt gctcggcgta gtggcggtcg gccagatccg cgtcgatggt gcgctgctgc
     2401 agggctgcga cgcggaggcc cttgctctcg taccgggcaa gaatggcgcc gaccaggccg
     2461 cggcgcaccg cgtccggctt gatgatgaca aaggtgcgat cgctcatgac gcaccaccct
     2521 accggcggcg accgacggtt ctacccgacc gttcgcgcct ccaaccgctt gcccagcacg
     2581 aacgtgatca cgaacagcag caggaacatg ccaccgacgg cgaacatcat ctcgaccacg
     2641 aaaccgagcg cgacgcacgc cacttgcgcg gcccaggcca gcggccagcc gtagggacgc
     2701 cgcagcgtgc cggc
//
LOCUS       DAOSUG010000444         2706 bp    DNA     linear   ENV 10-JUN-2024
DEFINITION  MAG TPA_asm: Micropruina sp. isolate AMR_MDS_2195
            NODE_15177_length_2706_cov_3.823463, whole genome shotgun sequence.
ACCESSION   DAOSUG010000444 DAOSUG010000000
VERSION     DAOSUG010000444.1
DBLINK      BioProject: PRJNA925477
            BioSample: SAMN32864262
            Sequence Read Archive: SRR4236673
KEYWORDS    WGS; ENV; Metagenome Assembled Genome; MAG; Third Party Data; TPA;
            TPA:assembly.
SOURCE      Micropruina sp. (wastewater metagenome)
  ORGANISM  Micropruina sp.
            Bacteria; Bacillati; Actinomycetota; Actinomycetes;
            Propionibacteriales; Nocardioidaceae; Micropruina.
REFERENCE   1  (bases 1 to 2706)
  AUTHORS   Abdulkadir,N., Saraiva,J.P., Zhang,J., Stolte,S., Gillor,O.,
            Harms,H. and Rocha,U.
  TITLE     Genome-centric analyses of 165 metagenomes show that mobile genetic
            elements are crucial for the transmission of antimicrobial
            resistance genes to pathogens in activated sludge and wastewater
  JOURNAL   Microbiol Spectr 12 (3), e0291823 (2024)
   PUBMED   38289113
REFERENCE   2  (bases 1 to 2706)
  AUTHORS   Abdulkadir,N.
  TITLE     Direct Submission
  JOURNAL   Submitted (24-MAR-2023) Environmental Microbiology, Helmholtz
            Center for Environmental Research -UFZ, Permoserstr 15, Leipzig
            04318, Germany
COMMENT     The annotation was added by the NCBI Prokaryotic Genome Annotation
            Pipeline (PGAP). Information about PGAP can be found here:
            https://www.ncbi.nlm.nih.gov/genome/annotation_prok/
            
            ##Genome-Assembly-Data-START##
            Assembly Method        :: SPAdes v. 3.11.1
            Genome Representation  :: Full
            Expected Final Version :: Yes
            Genome Coverage        :: Not Applicable
            Sequencing Technology  :: Illumina MiSeq
            ##Genome-Assembly-Data-END##
            
            ##Genome-Annotation-Data-START##
            Annotation Provider               :: NCBI
            Annotation Date                   :: 05/04/2023 04:57:10
            Annotation Pipeline               :: NCBI Prokaryotic Genome
                                                 Annotation Pipeline (PGAP)
            Annotation Method                 :: Best-placed reference protein
                                                 set; GeneMarkS-2+
            Annotation Software revision      :: 6.5
            Features Annotated                :: Gene; CDS; rRNA; tRNA; ncRNA
            Genes (total)                     :: 2,776
            CDSs (total)                      :: 2,750
            Genes (coding)                    :: 2,714
            CDSs (with protein)               :: 2,714
            Genes (RNA)                       :: 26
            tRNAs                             :: 24
            ncRNAs                            :: 2
            Pseudo Genes (total)              :: 36
            CDSs (without protein)            :: 36
            Pseudo Genes (ambiguous residues) :: 0 of 36
            Pseudo Genes (frameshifted)       :: 2 of 36
            Pseudo Genes (incomplete)         :: 32 of 36
            Pseudo Genes (internal stop)      :: 2 of 36
            ##Genome-Annotation-Data-END##
FEATURES             Location/Qualifiers
     source          1..2706
                     /organism="Micropruina sp."
                     /mol_type="genomic DNA"
                     /submitter_seqid="NODE_15177_length_2706_cov_3.823463"
                     /isolate="AMR_MDS_2195"
                     /isolation_source="Activated sludge of municipal
                     wastewater treatment plants"
                     /db_xref="taxon:2737536"
                     /environmental_sample
                     /geo_loc_name="Canada"
                     /lat_lon="49.25 N 123.1 W"
                     /collection_date="2015-06-16"
                     /metagenome_source="wastewater metagenome"
                     /note="metagenomic"
     gene            53..1084
                     /locus_tag="PLF56_12490"
     CDS             53..1084
                     /locus_tag="PLF56_12490"
                     /inference="COORDINATES: protein motif:HMM:NF021778.2"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="beta-propeller fold lactonase family protein"
                     /protein_id="HOQ54416.1"
                     /translation="MTPSWLVLVANSGDGTITSFSLTDGRLHRLAVNAGVPGCSTFVV
                     DSRRDLVYAAVKGDDPGIATLALDRSSGRLWVRSHLSLPDGSMNYLTLTRAGSGLLGA
                     SYGGGYGISCTVDDGVVSAPVGRVVYPNLHSVRTSSDGRFAYFVSLGADLIAQCALDD
                     SLNLVPLHHPTVDAPKGSGPRHLVLNAAQDQLYVLTEFSGEVLRYRRDQHYGTLTAAG
                     ATSAVDPSKNLGHSEFGADPVQHHYIWGADLHFSDPAHLWCSERTESTLGAVGVAPDG
                     SLTPPTSFVVTEPQPRGFAVSPDGRYLVCAGERSTTISLYRVRGADLELREQAETGTK
                     ANWVWVTAR"
     gene            complement(1097..2425)
                     /locus_tag="PLF56_12495"
     CDS             complement(1097..2425)
                     /locus_tag="PLF56_12495"
                     /inference="COORDINATES: ab initio
                     prediction:GeneMarkS-2+"
                     /note="Derived by automated computational analysis using
                     gene prediction method: GeneMarkS-2+."
                     /codon_start=1
                     /transl_table=11
                     /product="hypothetical protein"
                     /protein_id="HOQ54417.1"
                     /translation="MSLPLSRRTALGGAAALGVTAPLALSAAPARATSNRRPRADVKA
                     ATTFLQRVVDAYRSTGFRLAQSYQDDSGLNDIAFVYDNALAIIALLAAGDKPRARAIG
                     DALLYAQAHDETHTDHRLRQAYHANTFVNDNGTVHFGWEFGLTGTAVGDMAWAGIALA
                     QLARTTGRPEYLQGAVRIGQWIQNNTHSTTGLKGYTFGETAGLEQHKSTEHNIDVFAF
                     FNLLRRLTGQMAWSRRADHAWAFVQRVWNEAGGFFWTGSDDGATINKAAGQLPLDVQT
                     WSWLAARQSGYGKALDWARTNLASTDTPLRANSALSGNVSLSGAVFASGSLLADPTQN
                     IGGQSWNPKPDVAAVWLEGTAQLALALADRGDRGDADRADALLDQLSSAQAALGQTQK
                     FGGRVNPGGVVAASSPLDTGFGFGYFPNLHVGATSWYLMALLETNPYRFV"
BASE COUNT          381 a          898 c          983 g          444 t
ORIGIN      
        1 acgctggtcg agcagcgctc cggcgggggg cgtccggccc ataggatgag gtatgacccc
       61 ctcgtggctc gttctggtcg ccaattcggg tgacggcacc atcacgtcct tctcgctcac
      121 cgacggccgg ctgcaccggc tggcggtcaa cgccggcgtg ccgggttgct ccaccttcgt
      181 cgtggattcg cgtagagacc tggtctacgc cgcggtgaag ggcgacgatc cgggcatcgc
      241 gacgctggcc ctggaccgtt cgtcgggacg gctgtgggtg cgttcacatc tctcactgcc
      301 ggacggctcg atgaactacc tgaccctgac ccgggccggc agcggcctgt tgggagcttc
      361 ctacggcggc ggctacggca tcagctgcac ggtggacgac ggggtggtca gcgcgccggt
      421 cgggcgggtt gtgtacccca acctgcactc ggtccgcacc agcagcgacg gccggttcgc
      481 gtactttgtg tcgttgggtg ccgacctgat cgcccagtgc gcgctggacg actcgctgaa
      541 cctggtgccg ctgcatcacc cgaccgtcga tgccccgaag ggttcgggcc cccggcatct
      601 ggtgttgaac gccgcccagg atcagctgta cgtgctcacc gagttctcgg gtgaggtgtt
      661 gcgttaccgc cgcgaccagc actacggcac cctgaccgcc gcgggcgcca cctcggccgt
      721 cgatccgtcc aagaacctgg ggcacagcga gttcggcgcc gacccggtgc agcaccacta
      781 catctggggc gccgacctgc acttctcgga ccctgcgcac ctgtggtgct cggagcgcac
      841 cgagagcacc ctgggcgccg tcggcgtggc cccggacgga tcgctgactc cgccgacgag
      901 cttcgtggtc accgaaccgc agccccgcgg tttcgcggtg tccccggacg gccgctacct
      961 ggtctgcgcg ggggaacgct cgaccacgat ttcgctctac cgcgtgcgcg gtgccgatct
     1021 cgagctgcgt gaacaggccg agaccggcac caaggccaac tgggtgtggg tgaccgcccg
     1081 gtagggatgc ggggcgctag acgaaccggt aggggttggt ctcgagcagc gccatcaggt
     1141 accacgaggt ggcgccgaca tgcaggttgg gaaagtaccc gaagccgaat ccggtatcga
     1201 gcggcgagct cgcggcgacc acgccacccg ggttcaccct gccaccgaac ttctgggtct
     1261 ggcccagcgc ggcctgcgcg gacgacagct ggtcgagcag ggcgtccgcg cgatcggcat
     1321 cgccgcggtc accgcggtcg gcgagcgcca gggcgagctg ggcggtgcct tcgagccaga
     1381 cggcggcgac gtcgggcttg gggttccacg actggccgcc gatgttctgc gtcgggtcgg
     1441 cgagcagcga gccggaggcg aacaccgcgc cgctgaggct gacgttgccg ctcagcgccg
     1501 agttggcccg cagcggggtg tcggtgctgg ccaggttggt gcgggcccag tccagggcct
     1561 tgccgtagcc cgactgccgg gcggccagcc acgaccaggt ctgcacgtcg agcggtagct
     1621 ggccggcggc cttgttgatc gtggcgccgt cgtccgagcc cgtccagaag aagccgccgg
     1681 cctcgttcca gacccgctgg acgaacgccc acgcatggtc ggcgcggcga ctccaagcca
     1741 tctgcccggt gagccggcgc agcaggttga agaacgcgaa cacgtcgatg ttgtgctcgg
     1801 tggacttgtg ctgctccaga cccgcggtct cgccgaaggt gtagcccttc agcccagtgg
     1861 tgctgtgcgt gttgttctgg atccattggc cgattcggac cgcaccctgc aggtactcgg
     1921 ggcgcccggt ggttcgggcc aactgcgcca gtgcgatgcc ggcccacgcc atgtcgccga
     1981 ccgcggtgcc ggtcagtccg aactcccagc cgaagtgcac cgtgccgttg tcgttgacga
     2041 aggtgttggc gtggtacgcc tgacgcaacc ggtggtcggt gtgggtttcg tcgtgggcct
     2101 gcgcgtacag cagggcgtcg ccgatggccc gggcccgcgg cttgtcaccg gcggccagca
     2161 gggcgatgat cgccaacgcg ttgtcgtaga cgaaggcgat gtcgttcagt cccgagtcgt
     2221 cctgatagct ctgggcgagc cgaaaaccgg tgctgcggta ggcgtccacg acgcgttgca
     2281 gaaacgtggt ggcggccttg acgtcggctc gcggacggcg gttggacgtg gcgcgcgccg
     2341 gtgcggcgga caacgcgagc ggggcggtga cgccgagggc ggcggcaccc ccgagggcag
     2401 tgcgacggga cagcggaagc gacatcgatt cctccaggtg agtgtggtga gagcgctctc
     2461 agtcttgcgc gctcgccatt ggctggcaag ggatttcggt tgtctggcgg ggatcccggc
     2521 gttcgccgga tgacgacttg acgctcgtca gtcctgctcg tccgcccagg ccttggtcag
     2581 gccgcggaag tgggccagga accggtcgtg ctcgtgcggc ggatacgtgg cccgcacggt
     2641 gtcgacgagc aggcggtcga acgcacccga acgcacccac tcgagcacgg ccgcgtccag
     2701 atggcc
//
LOCUS       DAOSUG010000445         2702 bp    DNA     linear   ENV 10-JUN-2024
DEFINITION  MAG TPA_asm: Micropruina sp. isolate AMR_MDS_2195
            NODE_15204_length_2702_cov_6.047979, whole genome shotgun sequence.
ACCESSION   DAOSUG010000445 DAOSUG010000000
VERSION     DAOSUG010000445.1
DBLINK      BioProject: PRJNA925477
            BioSample: SAMN32864262
            Sequence Read Archive: SRR4236673
KEYWORDS    WGS; ENV; Metagenome Assembled Genome; MAG; Third Party Data; TPA;
            TPA:assembly.
SOURCE      Micropruina sp. (wastewater metagenome)
  ORGANISM  Micropruina sp.
            Bacteria; Bacillati; Actinomycetota; Actinomycetes;
            Propionibacteriales; Nocardioidaceae; Micropruina.
REFERENCE   1  (bases 1 to 2702)
  AUTHORS   Abdulkadir,N., Saraiva,J.P., Zhang,J., Stolte,S., Gillor,O.,
            Harms,H. and Rocha,U.
  TITLE     Genome-centric analyses of 165 metagenomes show that mobile genetic
            elements are crucial for the transmission of antimicrobial
            resistance genes to pathogens in activated sludge and wastewater
  JOURNAL   Microbiol Spectr 12 (3), e0291823 (2024)
   PUBMED   38289113
REFERENCE   2  (bases 1 to 2702)
  AUTHORS   Abdulkadir,N.
  TITLE     Direct Submission
  JOURNAL   Submitted (24-MAR-2023) Environmental Microbiology, Helmholtz
            Center for Environmental Research -UFZ, Permoserstr 15, Leipzig
            04318, Germany
COMMENT     The annotation was added by the NCBI Prokaryotic Genome Annotation
            Pipeline (PGAP). Information about PGAP can be found here:
            https://www.ncbi.nlm.nih.gov/genome/annotation_prok/
            
            ##Genome-Assembly-Data-START##
            Assembly Method        :: SPAdes v. 3.11.1
            Genome Representation  :: Full
            Expected Final Version :: Yes
            Genome Coverage        :: Not Applicable
            Sequencing Technology  :: Illumina MiSeq
            ##Genome-Assembly-Data-END##
            
            ##Genome-Annotation-Data-START##
            Annotation Provider               :: NCBI
            Annotation Date                   :: 05/04/2023 04:57:10
            Annotation Pipeline               :: NCBI Prokaryotic Genome
                                                 Annotation Pipeline (PGAP)
            Annotation Method                 :: Best-placed reference protein
                                                 set; GeneMarkS-2+
            Annotation Software revision      :: 6.5
            Features Annotated                :: Gene; CDS; rRNA; tRNA; ncRNA
            Genes (total)                     :: 2,776
            CDSs (total)                      :: 2,750
            Genes (coding)                    :: 2,714
            CDSs (with protein)               :: 2,714
            Genes (RNA)                       :: 26
            tRNAs                             :: 24
            ncRNAs                            :: 2
            Pseudo Genes (total)              :: 36
            CDSs (without protein)            :: 36
            Pseudo Genes (ambiguous residues) :: 0 of 36
            Pseudo Genes (frameshifted)       :: 2 of 36
            Pseudo Genes (incomplete)         :: 32 of 36
            Pseudo Genes (internal stop)      :: 2 of 36
            ##Genome-Annotation-Data-END##
FEATURES             Location/Qualifiers
     source          1..2702
                     /organism="Micropruina sp."
                     /mol_type="genomic DNA"
                     /submitter_seqid="NODE_15204_length_2702_cov_6.047979"
                     /isolate="AMR_MDS_2195"
                     /isolation_source="Activated sludge of municipal
                     wastewater treatment plants"
                     /db_xref="taxon:2737536"
                     /environmental_sample
                     /geo_loc_name="Canada"
                     /lat_lon="49.25 N 123.1 W"
                     /collection_date="2015-06-16"
                     /metagenome_source="wastewater metagenome"
                     /note="metagenomic"
     gene            200..1744
                     /gene="istA"
                     /locus_tag="PLF56_12500"
     CDS             200..1744
                     /gene="istA"
                     /locus_tag="PLF56_12500"
                     /inference="COORDINATES: protein motif:HMM:NF033546.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_function: GO:0004803 - transposase activity [Evidence
                     IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="IS21 family transposase"
                     /protein_id="HOQ54418.1"
                     /translation="MEQFERIRRDRRDEGLSIRELARKHRVHRRTVRAALADAVPPPR
                     KVPEREAPVTGAHVELVRSWLTQDLDAPRKQRHTARRVWQRLMEEEGAVVAESTVRNL
                     VAGLRAEVAGSRAQVMIVQEHPPGEEAEVDFGEFRASIGGVMLRLWMFCMRLSHSGRA
                     FHYAYANQAQESFLDGHVRAFAAFGGTPSGMIRYDNLKPAVIRCALGRERFEHPRFVA
                     LRSHYGFDSFYCQPGIEGAHEKGGVEGEIGRFRRRHLTPVPHVGSLSALNEAMAAAEA
                     RDDARRIGARPVTVGAMFAVEAPLLLALPPTPFDASAALSVRVDAKARVCVRQSYYSV
                     PARYAGQRLDVALGATTVRVLQGGSLVAEHVRSLHKGSQDLVLDHYLEVLTRKPGALA
                     GSSALATARANKTFTPTHEAYWKAARARLGDSGGTRALIEALLLHRTLPTGAVLAGIT
                     EATAAGVFDADVIAMTARRFLHAATSPPSPPPVPVPEGAPPAALVHRPAPCLGSYDQL
                     LTGAGA"
     gene            1741..2541
                     /gene="istB"
                     /locus_tag="PLF56_12505"
     CDS             1741..2541
                     /gene="istB"
                     /locus_tag="PLF56_12505"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_020578919.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_function: GO:0005524 - ATP binding [Evidence IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="IS21-like element helper ATPase IstB"
                     /protein_id="HOQ54419.1"
                     /translation="MSAATPAVTALGADAAQAAVTAAAKALGLPTVREEAARYATAAA
                     KARLTHLAFLAEVLSAECDDREHRRRQRRINEAKFPRHKRLSDFDPARLPDLPAATLA
                     HLGGGAWIEAGQPLVLLGDSGTGKTHLLIALGMAAAEQGHRVRYITTAALVNELTEAA
                     DDKQLTRVVNRYARLDLLCLDEIGYVHLDPRGAELLFQIITAREERASIACASNAPFS
                     EWGNTFTDPRLAAAVVDRLTFNAHIINTGTQSYRLATTRTRQEVTPPS"
BASE COUNT          422 a          993 c          910 g          377 t
ORIGIN      
        1 atgacctggc caagtacttc cactggcacg gcaacgcgtt cactgacggc caggtgagtg
       61 tgccagtctg aagtggcccc acttggaggc gtagtgctgg tctgaagtgg ccccaccctc
      121 gaccccgaac ttccctctac tgtccggatc gtctgccaag gcgatcacgg aggtgtggag
      181 ggtgaaggag agatcgagag tggagcagtt cgaacgtatc cgacgcgacc ggcgcgatga
      241 aggcctgtcg atcagagagc tcgccaggaa gcaccgggtg caccggcgca cggtgcgtgc
      301 ggcgttggct gacgcggtcc caccgccccg gaaggtgccc gagcgtgagg cgccggtcac
      361 gggtgcgcac gtggagctcg tgcggtcctg gttgacgcag gacctggacg cgccgcgcaa
      421 gcagcggcac accgcccgcc gggtgtggca gcggctgatg gaggaggaag gagcggtggt
      481 ggccgagtcc acggtgcgca acctcgtggc gggtcttcgt gccgaggtcg ccgggtctcg
      541 ggcccaggtg atgatcgtcc aggagcaccc gccgggtgag gaggccgagg tcgacttcgg
      601 tgagttccgc gccagcatag gcggggtgat gctgcggctg tggatgttct gcatgcgcct
      661 gtcgcactcc ggccgtgcgt tccactacgc ctacgccaac caggcccagg agtctttcct
      721 ggacgggcac gtgcgcgcgt tcgccgcgtt cggtgggaca ccgagcggga tgatccgcta
      781 cgacaacctc aagcccgcgg tgatccggtg cgcgttgggt cgggaacggt tcgagcaccc
      841 tcgcttcgtg gcgttgcggt cgcactacgg cttcgactcc ttctactgcc agcccgggat
      901 cgagggcgcg cacgagaagg gcggggtcga gggagagatc ggccggttcc gccgccggca
      961 cctgaccccg gtcccgcacg tgggctccct gtccgcgttg aacgaggcga tggccgcggc
     1021 cgaagcccgt gacgacgccc gccgcatcgg tgcccgcccg gtcacggtcg gggccatgtt
     1081 cgcggtcgag gcgccgctgc tgctggcgtt gccaccgacc ccgttcgacg cttcggcggc
     1141 gctgtcggtg cgggtggacg ccaaggcgcg ggtgtgtgtg cgccagtcgt actactcggt
     1201 cccggcccgc tacgccggtc aacggctgga cgtcgcgctc ggcgccacca ccgtccgggt
     1261 actccagggt ggcagcctgg tggccgagca cgtccgctcc ctgcacaagg gctcccagga
     1321 cctggtactg gaccactacc tggaggtcct gacccgcaaa cccggcgccc tggccggctc
     1381 gagcgcgttg gcgaccgccc gggcgaacaa gacattcacc ccgacccacg aggcgtactg
     1441 gaaggccgcc cgtgcacgct tgggtgacag cggcggcacc agggcgttga tcgaggcgct
     1501 gctgctgcac cgcaccctgc ccaccggtgc cgtcctggcc gggatcaccg aggccacggc
     1561 ggctggggtc ttcgacgccg acgtcatcgc catgaccgcc cggcggttcc tgcacgcggc
     1621 gaccagccca cccagcccgc cgccggtgcc cgtgcccgag ggtgccccac cagcggccct
     1681 ggtccaccgg ccggcgccct gcctgggttc ctacgaccag ctgctcaccg gggccggagc
     1741 atgagcgcgg ccacgccagc ggtcaccgcg ctcggtgccg acgcggccca ggccgcggtc
     1801 accgcggccg ccaaggccct ggggctgccg accgtgcggg aggaagccgc ccggtacgcg
     1861 accgcggcgg ccaaggcccg cctgacccac ctggcgttcc tggccgaggt gctctccgcc
     1921 gagtgcgacg accgtgagca ccggcgccgg caacgccgga tcaacgaggc caagttcccc
     1981 cgccacaaac gcctgtccga cttcgacccc gcccgcctgc ccgacctgcc cgcagcgacc
     2041 ctggcccacc tgggcggcgg tgcctggatc gaggccgggc aaccgctggt gctgctcggc
     2101 gactccggaa ccggcaagac gcatctgctc atcgccctgg gcatggccgc ggccgagcag
     2161 ggtcaccggg tccgctacat caccaccgcg gccctggtca acgaactgac cgaggccgcc
     2221 gacgacaagc agctcacccg ggtggtgaac aggtacgcac gactggacct gctgtgcctg
     2281 gacgagatcg gctacgtcca cctcgacccc cgcggggccg agctgctctt ccagatcatc
     2341 accgcccgcg aggaacgagc ctccatcgcc tgcgcttcta acgccccgtt cagcgagtgg
     2401 ggcaacacct tcaccgaccc ccgactggcc gccgccgtcg tcgaccggct caccttcaac
     2461 gcccacatca tcaacaccgg cacccagtcc taccgactcg ccaccacccg cacccgccag
     2521 gaggtgaccc caccaagctg accccgccca accccggcga ccgagcaaaa ccaacatgcc
     2581 tcagcaaccc cgagggtggg gccaaatcaa aacagcacag cggggccaaa tggacttgtc
     2641 attctcagcc aggtcatcga caccatcacg ctgcgccgcc agaaggctga tcccgaccag
     2701 tt
//
LOCUS       DAOSUG010000446         2700 bp    DNA     linear   ENV 10-JUN-2024
DEFINITION  MAG TPA_asm: Micropruina sp. isolate AMR_MDS_2195
            NODE_15223_length_2700_cov_4.471834, whole genome shotgun sequence.
ACCESSION   DAOSUG010000446 DAOSUG010000000
VERSION     DAOSUG010000446.1
DBLINK      BioProject: PRJNA925477
            BioSample: SAMN32864262
            Sequence Read Archive: SRR4236673
KEYWORDS    WGS; ENV; Metagenome Assembled Genome; MAG; Third Party Data; TPA;
            TPA:assembly.
SOURCE      Micropruina sp. (wastewater metagenome)
  ORGANISM  Micropruina sp.
            Bacteria; Bacillati; Actinomycetota; Actinomycetes;
            Propionibacteriales; Nocardioidaceae; Micropruina.
REFERENCE   1  (bases 1 to 2700)
  AUTHORS   Abdulkadir,N., Saraiva,J.P., Zhang,J., Stolte,S., Gillor,O.,
            Harms,H. and Rocha,U.
  TITLE     Genome-centric analyses of 165 metagenomes show that mobile genetic
            elements are crucial for the transmission of antimicrobial
            resistance genes to pathogens in activated sludge and wastewater
  JOURNAL   Microbiol Spectr 12 (3), e0291823 (2024)
   PUBMED   38289113
REFERENCE   2  (bases 1 to 2700)
  AUTHORS   Abdulkadir,N.
  TITLE     Direct Submission
  JOURNAL   Submitted (24-MAR-2023) Environmental Microbiology, Helmholtz
            Center for Environmental Research -UFZ, Permoserstr 15, Leipzig
            04318, Germany
COMMENT     The annotation was added by the NCBI Prokaryotic Genome Annotation
            Pipeline (PGAP). Information about PGAP can be found here:
            https://www.ncbi.nlm.nih.gov/genome/annotation_prok/
            
            ##Genome-Assembly-Data-START##
            Assembly Method        :: SPAdes v. 3.11.1
            Genome Representation  :: Full
            Expected Final Version :: Yes
            Genome Coverage        :: Not Applicable
            Sequencing Technology  :: Illumina MiSeq
            ##Genome-Assembly-Data-END##
            
            ##Genome-Annotation-Data-START##
            Annotation Provider               :: NCBI
            Annotation Date                   :: 05/04/2023 04:57:10
            Annotation Pipeline               :: NCBI Prokaryotic Genome
                                                 Annotation Pipeline (PGAP)
            Annotation Method                 :: Best-placed reference protein
                                                 set; GeneMarkS-2+
            Annotation Software revision      :: 6.5
            Features Annotated                :: Gene; CDS; rRNA; tRNA; ncRNA
            Genes (total)                     :: 2,776
            CDSs (total)                      :: 2,750
            Genes (coding)                    :: 2,714
            CDSs (with protein)               :: 2,714
            Genes (RNA)                       :: 26
            tRNAs                             :: 24
            ncRNAs                            :: 2
            Pseudo Genes (total)              :: 36
            CDSs (without protein)            :: 36
            Pseudo Genes (ambiguous residues) :: 0 of 36
            Pseudo Genes (frameshifted)       :: 2 of 36
            Pseudo Genes (incomplete)         :: 32 of 36
            Pseudo Genes (internal stop)      :: 2 of 36
            ##Genome-Annotation-Data-END##
FEATURES             Location/Qualifiers
     source          1..2700
                     /organism="Micropruina sp."
                     /mol_type="genomic DNA"
                     /submitter_seqid="NODE_15223_length_2700_cov_4.471834"
                     /isolate="AMR_MDS_2195"
                     /isolation_source="Activated sludge of municipal
                     wastewater treatment plants"
                     /db_xref="taxon:2737536"
                     /environmental_sample
                     /geo_loc_name="Canada"
                     /lat_lon="49.25 N 123.1 W"
                     /collection_date="2015-06-16"
                     /metagenome_source="wastewater metagenome"
                     /note="metagenomic"
     gene            <1..1188
                     /locus_tag="PLF56_12510"
     CDS             <1..1188
                     /locus_tag="PLF56_12510"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_015069300.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_function: GO:0005524 - ATP binding [Evidence IEA];
                     GO_function: GO:0042626 - ATPase-coupled transmembrane
                     transporter activity [Evidence IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="ABC transporter ATP-binding protein"
                     /protein_id="HOQ54420.1"
                     /translation="ATQQIRIDTLNRVLREQISGLRVIRAFTAEQRENDRFAVANAEL
                     TDTATRVGRRMMTLFPTVFFVMNVSTIGVTWFGAFQVAGGAMETGQLLAFIQYLTTIL
                     MSVMMATMMLMIAPRAAVSAGRIAEVLDTESSVVTSSNPVRAVTQRGHVELQNVEFRY
                     PGAQQPVLCGINLEVKPGQTTAVIGSTGAGKTTLISLIPRLFDATGGRVLVDGVDVRD
                     LEPDLLWSRIGLVPQKPYLFTGTVASNLRYGNPDATDDELWAALRTAQADDFVRAMPG
                     QLEAPIAQGGTNVSGGQRQRLSIARALVRKPDIYIFDDSFSALDVVTDARLRAALDRE
                     TAGAGVLIVAQRVSTIRDAETIVVLDGGRIDGVGTHEQLLDTCPTYAEIVASQFKAEE
                     AAA"
     gene            1185..>2700
                     /locus_tag="PLF56_12515"
     CDS             1185..>2700
                     /locus_tag="PLF56_12515"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_013861152.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="ABC transporter ATP-binding protein"
                     /protein_id="HOQ54421.1"
                     /translation="MSQSPRAPENASDASGTVGAGLVPGTSGNTPAGASDTTPKANAR
                     PEHAEHQRRGMGHGPMQGMGSSEKAVSFGPSAQRLLGLMRPERNLIMVVIVISALATL
                     GNALGPVILAQGTNLIFDGATKGTGIDFGALATVIWQALAVYVIAGLVLWFASFLIND
                     IVQRTVYRMREQVATKLHRLPITYFDGQPRGELLSRVTNDIDNIAQSLQQTLAQMLNN
                     IFTVIGVLALMFWLSPPLALVVLVSVPLTVLVTAQVMKRSQSRFADMWRSTGKLNAEV
                     EEAFTGHSLVKVFGRQREVAARFAEQNHSLYDSSFKAQFISGIIMPCVFFIGNLSYVA
                     IAVIGSIWVAAGTLTLGVVQAFIQYARMFTQPITQLASMTNLLQSGVASAERVFEVLD
                     ATEQSIDGNQHLPKPLEGRVVFDHVSFSYDPAKPLITDLSLVAEPGSTVAIVGPTGAG
                     KTTLVNLIMRFYEVDSGRITIDGVDIAEVPRRELRENIGMVLQDAWLFGGTIRDN"
BASE COUNT          446 a          945 c          879 g          430 t
ORIGIN      
        1 gccacccagc agatccgcat cgacaccctc aaccgggtgc tgcgcgagca gatctcgggc
       61 ctgcgggtga tccgggcctt caccgccgaa cagcgtgaga acgaccggtt cgcagtggcc
      121 aacgccgaac tgaccgacac cgccacccgg gtcggccggc gcatgatgac gctgttcccg
      181 accgtgttct tcgtgatgaa cgtctcgacc atcggggtga cctggttcgg cgccttccag
      241 gtggccggcg gcgcgatgga aaccgggcag ctgctcgcct tcatccagta cctgaccacc
      301 attctgatga gcgtgatgat ggcaacgatg atgctgatga tcgcgccccg cgccgccgtc
      361 agcgccggcc ggatcgccga ggtgctcgac accgagtcca gcgtcgtcac ctcatcgaat
      421 cccgtccgcg ccgtcaccca gcggggccac gtcgagctgc agaacgtcga gttccgctac
      481 ccgggcgcgc agcagccggt gctgtgcggc atcaacctcg aggtcaagcc cggccagacc
      541 accgccgtga tcggttccac cggcgccggg aagaccacgc tgatcagcct gatcccgcgc
      601 ctgttcgacg ccaccggcgg acgcgtgctg gtcgacggcg tggacgtccg cgacctcgag
      661 cccgacctgc tctggtcgcg gatcgggttg gtgccgcaga agccgtacct gttcaccggc
      721 accgtggcgt ccaacctgcg ctacggcaat ccggacgcca ccgacgacga gctgtgggcc
      781 gcgctgcgca cggcgcaggc cgacgacttc gtccgggcga tgccggggca gttggaggcg
      841 cccatcgctc agggcggcac caacgtgtcc ggcggtcagc ggcaacggct ctcgatcgcg
      901 cgtgccctgg tcagaaagcc cgacatctac atcttcgacg actcgttctc ggcgctggac
      961 gtggtcaccg acgccaggct gcgggcagcc ctggatcgtg agaccgccgg ggccggggtg
     1021 ctgatcgtcg cccagcgggt ctcgaccatc cgcgacgcgg aaaccatcgt cgtgctcgac
     1081 ggcgggcgca tcgacggggt gggcacccac gagcagttgc tcgatacctg ccccacctac
     1141 gccgagatcg tggcgtccca gttcaaggcc gaggaggcag cagcatgagc cagtcacccc
     1201 gcgcacctga gaacgcatcg gacgccagcg gcaccgtcgg tgccggcctc gtccccggca
     1261 cctcaggcaa cacacccgcc ggcgcgtccg acaccacacc caaggccaac gcccgtcccg
     1321 agcatgccga gcaccagcgc cgcggcatgg gtcacgggcc gatgcagggc atgggctcgt
     1381 ccgagaaggc ggtcagcttc ggcccgtccg ctcaacgact gctgggcctg atgcgtcccg
     1441 aacgcaacct gatcatggtg gtgatcgtga tctcggccct cgccacgctg ggcaacgcgt
     1501 tgggtccggt gatcctggcc cagggcacca acctgatctt cgacggcgcc accaagggca
     1561 ccggcatcga cttcggcgcc ctggcgacgg tgatctggca ggccctggcc gtctacgtga
     1621 tcgccggcct ggtgctgtgg ttcgccagct ttctgatcaa cgacatcgtg cagcgcaccg
     1681 tctaccgcat gcgcgagcag gtggccacca aactgcaccg gctgccgatc acctacttcg
     1741 acggccagcc ccgcggcgag ctgctgagcc gggtgaccaa cgacatcgac aacatcgccc
     1801 agtcgctgca gcagaccctg gcgcaaatgc tgaacaacat cttcaccgtg atcggtgtgc
     1861 tcgcgctgat gttctggctg tcgccgcccc tggcgctggt ggtgctggtg tcggtgccgc
     1921 tgaccgtgct ggtcaccgcg caggtgatga agcgttcgca gagccggttc gccgacatgt
     1981 ggcgctcgac cggcaagctg aacgcagagg tcgaagaggc gttcaccggg cactcgctgg
     2041 tgaaggtgtt cggccggcag cgcgaggtcg ccgcgcgctt cgccgagcag aaccactcgc
     2101 tctacgactc gtcgttcaag gcccagttca tcagcggcat catcatgccc tgcgtgttct
     2161 tcatcggaaa cctgagctac gtggccatcg cggtgatcgg cagcatctgg gtggccgccg
     2221 gcaccctgac cctgggcgtg gtgcaggcgt tcattcagta cgcccgcatg ttcacccagc
     2281 cgatcaccca gctggcgtcg atgaccaacc tgctgcagtc tggggtcgcc tcggccgaac
     2341 gggtcttcga ggtgctggac gccaccgagc agtcgatcga cggcaaccaa cacctgccga
     2401 agccgctgga gggccgggtc gtgttcgatc acgtgtcgtt cagctacgac cccgccaagc
     2461 cgctgatcac ggacctgtcg ctggtcgccg agccgggttc gacggtggcg atcgtcgggc
     2521 cgaccggggc cggcaagacc accctggtga acctgatcat gcgcttctac gaggtcgatt
     2581 ccggacgcat cacgatcgac ggtgtcgaca tcgctgaggt gccaaggcgc gaactgcgcg
     2641 agaacatcgg catggtgctg caggacgcgt ggctgttcgg cggcacgatc cgcgacaaca
//
LOCUS       DAOSUG010000447         2692 bp    DNA     linear   ENV 10-JUN-2024
DEFINITION  MAG TPA_asm: Micropruina sp. isolate AMR_MDS_2195
            NODE_15289_length_2692_cov_4.871445, whole genome shotgun sequence.
ACCESSION   DAOSUG010000447 DAOSUG010000000
VERSION     DAOSUG010000447.1
DBLINK      BioProject: PRJNA925477
            BioSample: SAMN32864262
            Sequence Read Archive: SRR4236673
KEYWORDS    WGS; ENV; Metagenome Assembled Genome; MAG; Third Party Data; TPA;
            TPA:assembly.
SOURCE      Micropruina sp. (wastewater metagenome)
  ORGANISM  Micropruina sp.
            Bacteria; Bacillati; Actinomycetota; Actinomycetes;
            Propionibacteriales; Nocardioidaceae; Micropruina.
REFERENCE   1  (bases 1 to 2692)
  AUTHORS   Abdulkadir,N., Saraiva,J.P., Zhang,J., Stolte,S., Gillor,O.,
            Harms,H. and Rocha,U.
  TITLE     Genome-centric analyses of 165 metagenomes show that mobile genetic
            elements are crucial for the transmission of antimicrobial
            resistance genes to pathogens in activated sludge and wastewater
  JOURNAL   Microbiol Spectr 12 (3), e0291823 (2024)
   PUBMED   38289113
REFERENCE   2  (bases 1 to 2692)
  AUTHORS   Abdulkadir,N.
  TITLE     Direct Submission
  JOURNAL   Submitted (24-MAR-2023) Environmental Microbiology, Helmholtz
            Center for Environmental Research -UFZ, Permoserstr 15, Leipzig
            04318, Germany
COMMENT     The annotation was added by the NCBI Prokaryotic Genome Annotation
            Pipeline (PGAP). Information about PGAP can be found here:
            https://www.ncbi.nlm.nih.gov/genome/annotation_prok/
            
            ##Genome-Assembly-Data-START##
            Assembly Method        :: SPAdes v. 3.11.1
            Genome Representation  :: Full
            Expected Final Version :: Yes
            Genome Coverage        :: Not Applicable
            Sequencing Technology  :: Illumina MiSeq
            ##Genome-Assembly-Data-END##
            
            ##Genome-Annotation-Data-START##
            Annotation Provider               :: NCBI
            Annotation Date                   :: 05/04/2023 04:57:10
            Annotation Pipeline               :: NCBI Prokaryotic Genome
                                                 Annotation Pipeline (PGAP)
            Annotation Method                 :: Best-placed reference protein
                                                 set; GeneMarkS-2+
            Annotation Software revision      :: 6.5
            Features Annotated                :: Gene; CDS; rRNA; tRNA; ncRNA
            Genes (total)                     :: 2,776
            CDSs (total)                      :: 2,750
            Genes (coding)                    :: 2,714
            CDSs (with protein)               :: 2,714
            Genes (RNA)                       :: 26
            tRNAs                             :: 24
            ncRNAs                            :: 2
            Pseudo Genes (total)              :: 36
            CDSs (without protein)            :: 36
            Pseudo Genes (ambiguous residues) :: 0 of 36
            Pseudo Genes (frameshifted)       :: 2 of 36
            Pseudo Genes (incomplete)         :: 32 of 36
            Pseudo Genes (internal stop)      :: 2 of 36
            ##Genome-Annotation-Data-END##
FEATURES             Location/Qualifiers
     source          1..2692
                     /organism="Micropruina sp."
                     /mol_type="genomic DNA"
                     /submitter_seqid="NODE_15289_length_2692_cov_4.871445"
                     /isolate="AMR_MDS_2195"
                     /isolation_source="Activated sludge of municipal
                     wastewater treatment plants"
                     /db_xref="taxon:2737536"
                     /environmental_sample
                     /geo_loc_name="Canada"
                     /lat_lon="49.25 N 123.1 W"
                     /collection_date="2015-06-16"
                     /metagenome_source="wastewater metagenome"
                     /note="metagenomic"
     gene            complement(<1..850)
                     /locus_tag="PLF56_12520"
     CDS             complement(<1..850)
                     /locus_tag="PLF56_12520"
                     /EC_number="2.5.1.17"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_014847901.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_function: GO:0008817 - corrinoid adenosyltransferase
                     activity [Evidence IEA];
                     GO_process: GO:0009236 - cobalamin biosynthetic process
                     [Evidence IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="cob(I)yrinic acid a,c-diamide
                     adenosyltransferase"
                     /protein_id="HOQ54422.1"
                     /translation="MPNVYTRTGDTGLTGLFGGSRVPKLDPGVEAYGTVDEANSAIGE
                     AKALATDESIRATLHGIQQRLFVLAAELASDDAGRAILDNTISPADVKDLEDLIDMCL
                     SVTGPQRNFVVPGRDVPSGALHAARTVARRAERRVLALASQREVRPEVITYLNRLSDT
                     LFALARLSEHQFDQAHVTQVVREAVLKVLRAAGEIPGQARDDEATGDPTRHPGERRDL
                     DPTRAKAEIPGQARDDEWARRPGDSSGHADRSRHPGDSTRHPGERRDLNASPTTYDLA
                     ALKRMAE"
     gene            complement(852..1121)
                     /locus_tag="PLF56_12525"
     CDS             complement(852..1121)
                     /locus_tag="PLF56_12525"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_013160819.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="EutN/CcmL family microcompartment protein"
                     /protein_id="HOQ54423.1"
                     /translation="MFLAKVRGTVVSTSKDERLVGFKLLVVQRLGVEGEYLSYPQVAV
                     DTVGAGVGETVIVTGGSSARFAASRSDAPIDATIVGIVDSVEIGA"
     gene            complement(1123..1971)
                     /locus_tag="PLF56_12530"
     CDS             complement(1123..1971)
                     /locus_tag="PLF56_12530"
                     /inference="COORDINATES: protein motif:HMM:NF014494.2"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_function: GO:0003824 - catalytic activity [Evidence
                     IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="flavoprotein"
                     /protein_id="HOQ54424.1"
                     /translation="MTEHELRALIREVIGELIRPPQLRVLVVFTGGLLGFDEAIDSLA
                     ALKATGAHLDYRQTPSAERILDQRRIQALGMREVSKQYVESHDMLILPTMTGNIAAKV
                     AHGIADCLVSNLASEFIMSNRIVVASRTAVRPDGAEKLGWFPQMPPGYADLLRANLAA
                     LSSFGVRVVDAKALCRTAIAAWQRADRARRSPLVDALGTSPSAFTAQLSESDPVEAGG
                     DAAVAPAPTALPVGVVACPLSLISQQVVQQVSSGTELRIGRSAKVTAMALDLAGARGI
                     RISREV"
     gene            complement(1964..>2692)
                     /gene="eutJ"
                     /locus_tag="PLF56_12535"
     CDS             complement(1964..>2692)
                     /gene="eutJ"
                     /locus_tag="PLF56_12535"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_016668528.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_function: GO:0005515 - protein binding [Evidence IEA];
                     GO_process: GO:0051301 - cell division [Evidence IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="ethanolamine utilization protein EutJ"
                     /protein_id="HOQ54425.1"
                     /translation="DLGTANIVVSVVDDLDRPVAGGWVHSTVVRDGVVVDWAGATSAV
                     RKLTADVETRLGHRFTSASVSVPPGISEGDTKVFRNVAGAAGLDVTEVVDEPVAAARA
                     LGITDGCVIDVGHGTTGVSILRDGRVELSVDEATGGHHMTLVLAGAYRIGYDAAEAMK
                     KNPARRDDVLGVIRPTLEKMATIAQVALAGHDTPMVYLVGGSSSFPNAPDVFATRLGR
                     PVVRPVEPLFVTPLGIAMRKPDHD"
BASE COUNT          408 a          972 c          912 g          400 t
ORIGIN      
        1 cctcggccat gcgcttgagg gcggcgaggt cgtacgtcgt cggtgaggcg ttgagatccc
       61 ggcgttcgcc gggatgacgg gtggagtcgc cgggatgacg ggagcggtcg gcatgaccgg
      121 aggagtcgcc gggacgacgg gcccactcgt catcccgggc ttgccccggg atctcggcct
      181 tcgcccgtgt ggggtcgaga tcccggcgtt cgccgggatg acgggtgggg tcgccggtcg
      241 cttcgtcatc ccgggcttgc cccgggatct cgcccgccgc ccgcagcacc ttgagcactg
      301 cctcgcggac cacctgcgtg acgtgcgcct ggtcgaactg gtgctcgctc aatcgtgcca
      361 gcgcgaacag ggtgtccgag agccggttga ggtacgtgat cacctctgga cgcacctcgc
      421 gttgtgaggc gagagccagc acccggcgct cggcccggcg cgccaccgtc cgggcggcat
      481 gcagggcacc cgagggcacg tcgcgtccgg gcaccacgaa gttgcgctgc ggtccggtga
      541 ccgacaggca catgtcgatc aggtcttcga ggtccttcac gtcggccggg ctgatcgtgt
      601 tgtcgagaat ggcgcgaccc gcatcatcgc tggccagttc ggcggccagc acgaacagcc
      661 gctgctgaat gccgtgcagc gtcgcccgga tggactcgtc cgttgccagc gccttcgctt
      721 cgccgatcgc cgagttggcc tcgtccaccg tgccgtaggc ctcgacgcct gggtcgagct
      781 tgggcacccg cgaaccaccg aacaggccgg tcaggcccgt gtcgccggtg cgcgtgtaga
      841 cgttgggcat ctcaggcgcc gatctcgacc gaatcgacga ttccgacgat ggtggcgtcg
      901 atcggcgcgt ccgaacgaga ggccgcgaag cgagccgagc tgccgccggt cacgatcacc
      961 gtttcgccga ccccggcacc gaccgtgtcg acggccacct gcgggtagct cagatactcc
     1021 ccctcgacgc cgagccgctg caccacgagc agcttgaacc cgaccagccg ctcgtccttg
     1081 ctcgtcgaca ccaccgtgcc gcgcaccttc gccaggaaca tgtcacacct ctcgtgagat
     1141 ccgaatgccg cgagcgcccg ccaggtcgag cgccatcgcc gttaccttcg cggagcgtcc
     1201 gatgcgcaac tcggtaccgg acgacacctg ctgcaccacc tgctggctga tcaacgacag
     1261 cgggcacgcc accaccccca ccggcagcgc ggtcggcgca ggcgccacgg ccgcatcgcc
     1321 gcccgcctcg accgggtccg actcgctcaa ctgcgcggtg aacgccgacg gcgaggtgcc
     1381 cagcgcatcg accaacggtg agcggcgggc ccgatcggct cgctgccagg cggcgatcgc
     1441 cgtacggcac agggccttgg cgtcgaccac ccgcaccccg aacgacgaca acgcggccag
     1501 gttggcccgc aacagatcgg cgtagccggg cggcatctgc ggaaaccagc cgagcttctc
     1561 ggcgccgtcc ggacgcaccg cggtgcgcga cgcgaccacg atgcggttgc tcatgatgaa
     1621 ctcgctggcc aggttgctga ccaggcagtc ggctatgccg tgggccacct tggcggcgat
     1681 gttgccggtc atcgtcggca ggatcagcat gtcgtggctc tcgacgtact gcttcgacac
     1741 ctcgcgcatg ccgagggcct ggatgcgccg ctggtcgagg atgcgctcgg cgctcggggt
     1801 ctgccggtag tcgaggtgcg caccggtcgc cttgagcgcc gcgagcgaat cgatcgcctc
     1861 gtcgaaaccc agcagaccgc cggtgaacac caccagcacc cgcagttgcg gcggccggat
     1921 caactcgccg atcacctcac ggatcagcgc ccggagttca tgctcagtca tggtcgggtt
     1981 tcctcatcgc aatgccgagg ggggtgacga acaacggctc gaccggacgc accacgggcc
     2041 gtccgagccg ggtggcgaac acgtccgggg cgttcgggaa cgagctggag ccgccgacca
     2101 gatacaccat cggcgtgtca tgcccggcca acgccacctg ggcgatcgtg gccatcttct
     2161 ccaacgtcgg gcggatcacg cccagcacgt cgtcgcgccg ggccgggttc ttcttcatcg
     2221 cctcggcggc gtcgtagccg atccggtagg caccggccag caccagcgtc atgtggtgac
     2281 cgccggtggc ctcatcgacc gacagctcga cccggccgtc gcgcaggatc gacaccccgg
     2341 tcgtgccgtg gccgacgtcg atcacgcagc cgtcggtgat gcccagggcg cgggcggcgg
     2401 cgacgggttc gtccaccacc tcggtgacgt cgagaccggc ggccccggcc acgttgcgga
     2461 acaccttggt gtcgccctcg ctgatgcccg ggggcaccga caccgacgcg ctggtgaagc
     2521 ggtgacccag ccgggtctcc acgtcggcgg tcagcttgcg gacggcgctg gtcgcgcccg
     2581 cccagtcgac cacgaccccg tcccggacaa cggtggaatg cacccagccg ccggccaccg
     2641 gacgatcgag atcgtccacc accgacacca cgatgttcgc ggtaccgagg tc
//
LOCUS       DAOSUG010000448         2688 bp    DNA     linear   ENV 10-JUN-2024
DEFINITION  MAG TPA_asm: Micropruina sp. isolate AMR_MDS_2195
            NODE_15312_length_2688_cov_4.788454, whole genome shotgun sequence.
ACCESSION   DAOSUG010000448 DAOSUG010000000
VERSION     DAOSUG010000448.1
DBLINK      BioProject: PRJNA925477
            BioSample: SAMN32864262
            Sequence Read Archive: SRR4236673
KEYWORDS    WGS; ENV; Metagenome Assembled Genome; MAG; Third Party Data; TPA;
            TPA:assembly.
SOURCE      Micropruina sp. (wastewater metagenome)
  ORGANISM  Micropruina sp.
            Bacteria; Bacillati; Actinomycetota; Actinomycetes;
            Propionibacteriales; Nocardioidaceae; Micropruina.
REFERENCE   1  (bases 1 to 2688)
  AUTHORS   Abdulkadir,N., Saraiva,J.P., Zhang,J., Stolte,S., Gillor,O.,
            Harms,H. and Rocha,U.
  TITLE     Genome-centric analyses of 165 metagenomes show that mobile genetic
            elements are crucial for the transmission of antimicrobial
            resistance genes to pathogens in activated sludge and wastewater
  JOURNAL   Microbiol Spectr 12 (3), e0291823 (2024)
   PUBMED   38289113
REFERENCE   2  (bases 1 to 2688)
  AUTHORS   Abdulkadir,N.
  TITLE     Direct Submission
  JOURNAL   Submitted (24-MAR-2023) Environmental Microbiology, Helmholtz
            Center for Environmental Research -UFZ, Permoserstr 15, Leipzig
            04318, Germany
COMMENT     The annotation was added by the NCBI Prokaryotic Genome Annotation
            Pipeline (PGAP). Information about PGAP can be found here:
            https://www.ncbi.nlm.nih.gov/genome/annotation_prok/
            
            ##Genome-Assembly-Data-START##
            Assembly Method        :: SPAdes v. 3.11.1
            Genome Representation  :: Full
            Expected Final Version :: Yes
            Genome Coverage        :: Not Applicable
            Sequencing Technology  :: Illumina MiSeq
            ##Genome-Assembly-Data-END##
            
            ##Genome-Annotation-Data-START##
            Annotation Provider               :: NCBI
            Annotation Date                   :: 05/04/2023 04:57:10
            Annotation Pipeline               :: NCBI Prokaryotic Genome
                                                 Annotation Pipeline (PGAP)
            Annotation Method                 :: Best-placed reference protein
                                                 set; GeneMarkS-2+
            Annotation Software revision      :: 6.5
            Features Annotated                :: Gene; CDS; rRNA; tRNA; ncRNA
            Genes (total)                     :: 2,776
            CDSs (total)                      :: 2,750
            Genes (coding)                    :: 2,714
            CDSs (with protein)               :: 2,714
            Genes (RNA)                       :: 26
            tRNAs                             :: 24
            ncRNAs                            :: 2
            Pseudo Genes (total)              :: 36
            CDSs (without protein)            :: 36
            Pseudo Genes (ambiguous residues) :: 0 of 36
            Pseudo Genes (frameshifted)       :: 2 of 36
            Pseudo Genes (incomplete)         :: 32 of 36
            Pseudo Genes (internal stop)      :: 2 of 36
            ##Genome-Annotation-Data-END##
FEATURES             Location/Qualifiers
     source          1..2688
                     /organism="Micropruina sp."
                     /mol_type="genomic DNA"
                     /submitter_seqid="NODE_15312_length_2688_cov_4.788454"
                     /isolate="AMR_MDS_2195"
                     /isolation_source="Activated sludge of municipal
                     wastewater treatment plants"
                     /db_xref="taxon:2737536"
                     /environmental_sample
                     /geo_loc_name="Canada"
                     /lat_lon="49.25 N 123.1 W"
                     /collection_date="2015-06-16"
                     /metagenome_source="wastewater metagenome"
                     /note="metagenomic"
     gene            complement(72..1628)
                     /gene="istA"
                     /locus_tag="PLF56_12540"
     CDS             complement(72..1628)
                     /gene="istA"
                     /locus_tag="PLF56_12540"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_013349124.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_function: GO:0004803 - transposase activity [Evidence
                     IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="IS21 family transposase"
                     /protein_id="HOQ54426.1"
                     /translation="MVRKIKAKLVLRLRAEGFTGRQIAAQGMSRTSVAAVIEAADREG
                     IDWDAVAGMEESAVYARLFPGRGEHESVHAQPDWARVHRELARVGVTLKLLHGEYLDA
                     CRTEGSTAMGYDRFCKTYQRHVLVAGAASRVGHKAGQTVEVDWSGKTMRLTDPVTGAQ
                     ARVYLFVATLPFSRYAFVEPTVDMRQDTWLRAHVAMFEWLNGSVPRIVPDNLKTGVIK
                     HPAEGEVVLNDAYRELAGHYSAAVLPGRVRKPKDKASVENTVASVATWVIASLRDRQF
                     ASLAELRAAVYDRVDAFNQEPFQKRQGSRSSVFAAEEKPLLRPLPAVGFEISRWVYAR
                     KVQRNGHVVWEKNFYSVPYPHIGRSVDLRVTDTTLEVFTGDQRLTSHLLAPVGVVNEY
                     RTHDADLPDGPRYRQWDAQRVREWAARIGDNTTTVVNRIFESVPVDEQGLDAALAVLR
                     LTRRYSTARVEHACRIALSRVRSPRYAHLRPILETRQDHPDGTRPGPEPVDEQAGYVR
                     GADYYAGGSR"
     gene            complement(1772..2278)
                     /locus_tag="PLF56_12545"
     CDS             complement(1772..2278)
                     /locus_tag="PLF56_12545"
                     /inference="COORDINATES: ab initio
                     prediction:GeneMarkS-2+"
                     /note="Derived by automated computational analysis using
                     gene prediction method: GeneMarkS-2+."
                     /codon_start=1
                     /transl_table=11
                     /product="hypothetical protein"
                     /protein_id="HOQ54427.1"
                     /translation="MAVISVVGTAAGVFGVVQAMTRDTTNFSHLQISAGPTEGATAEW
                     AVPEDALDRLPDAGWCGEEMTSWLESNATPLRREVSIKLRNDATEGPMLALTDFRPVS
                     TVAEKRGPLRVRLVCDPAGSIPSQIYHGRVDADKGTTARHVRVSGTGAEAAPPVPVAF
                     NLAEYSHF"
BASE COUNT          433 a          985 c          818 g          452 t
ORIGIN      
        1 gtcgagggcg tcgaccaggg aagaggcgcc catctcccgc agcttgcgct tggtttcggt
       61 gtcgaggcgg ctcaccggga tcctcccgcg tagtagtcgg cgccgcgaac gtagcccgcc
      121 tgctcatcga ctggttcggg ccctggtctc gtgccgtcgg ggtggtcttg tctggtttcg
      181 aggatcggtc gtaggtgcgc gtagcgcggc gacctcaccc gggacaaggc gatccggcag
      241 gcgtgctcga ctcgggcggt ggagtagcgg cgtgtcagtc gtagcaccgc cagggcggcg
      301 tcgaggccct gctcgtcgac cggcacggac tcgaagatcc ggttgaccac tgtggtggtg
      361 ttgtcgccga tccgggcggc ccattcccga acccgttggg cgtcccactg ccggtagcgg
      421 ggcccgtcgg gcaggtccgc gtcgtgggtg cggtactcgt tgacgacacc gaccggcgcc
      481 agcagatggc tggtcagccg ctgatcgccg gtgaacacct cgagcgtcgt gtcggtgacc
      541 cgcagatcga ccgagcggcc gatgtgcggg taggggacgg agtagaagtt cttctcccag
      601 accacgtgcc cgttgcgttg gaccttgcgt gcgtagaccc agcgggagat ctcgaacccg
      661 accgcaggca gcggccgcag cagtggcttc tcctccgcgg cgaacacact cgacctggag
      721 ccttggcgtt tctggaacgg ttcctggttg aacgcgtcaa cccggtcgta gaccgccgcc
      781 cgtagctccg ccagcgacgc gaactgccgg tcgcggagcg aggcgatcac ccaggtcgcg
      841 acactggcga ccgtgttctc gacgctggcc ttgtccttcg gtttcctgac tctgccgggc
      901 aggaccgcgg ccgagtagtg cccggccaac tcccgatacg cgtcgttgag gaccacctcc
      961 ccttccgcgg gatgcttgat gacaccggtc ttcaggttgt ccggcacgat cctggggacg
     1021 ctgccgttca gccactcgaa catcgccacg tgcgcccgta gccacgtgtc ctgccgcatg
     1081 tcgaccgtcg gctcgacgaa cgcgtagcgg gagaacggca acgtcgccac gaacagatac
     1141 accctggcct gggcgccggt gaccggatcg gtcaaccgca tcgtcttccc tgaccagtcg
     1201 acctcgaccg tctgcccggc cttgtgaccc acccgcgacg cggcccccgc gaccaggaca
     1261 tgccgctgat aggtcttgca gaaccggtcg tagcccatcg cggtcgaccc ctccgtgcgg
     1321 caggcgtcca ggtactcgcc gtgcagcagc ttcaacgtca ccccgaccct cgcgagctcg
     1381 cgatgcaccc gagcccagtc tggctgcgca tgcacgctct catgctcccc acggcccggg
     1441 aacagccgcg catacacggc cgactcctcc atgcccgcca cggcatccca gtcgatgccc
     1501 tcccgatccg cggcttctat caccgccgcg acgctggtgc gggacatgcc ctgcgcggcg
     1561 atctgccgcc ccgtgaaccc ctcagcccga agccggagca ccagcttcgc cttgatcttg
     1621 cgtaccatcc gaacaactcc ttctgccgcg tgattggcca cacggcagaa ggagcctaga
     1681 aacggtggcc ctcaacccaa atatcgggtg gcacccaccg acgcgaacgc gtcagccggc
     1741 gactggcccc caagcgcaat atcggtggcc ctcagaagtg cgaatactca gcgaggttga
     1801 acgccaccgg aaccggtggg gccgcttcag cccccgtgcc gctcacccgg acatgccggg
     1861 ccgtggtccc tttgtccgca tccacccggc cgtggtagat ctggctgggg atcgaacctg
     1921 ccggatcgca caccagccgc accctcaacg gaccccgctt ctcggcgacg gtcgacaccg
     1981 gccggaagtc cgtcagcgcc agcatcggcc cctcggtcgc atcgttgcgc agcttgatcg
     2041 acacctcccg gcgcagcggc gtggcgttgc tctccagcca ggaggtcatc tcctcgccgc
     2101 accacccagc gtcaggcaac ctgtcgagtg cgtcctctgg cacggcccat tccgcggtcg
     2161 caccctccgt cggcccggcc gaaatctgca ggtgcgagaa attggtcgtg tcgcgcgtca
     2221 tcgcctgaac cacgccaaat acgccagcag ccgtcccgac gaccgaaatc accgccatga
     2281 cgacagcccc cgtccgcgac aagtgcgacc gcttgaccgt caggctcttc cccgtagtga
     2341 tttcgccgtc cgactctctg gcggcccctg acgctccttc gctctgctcg gctcgtgaag
     2401 gccgcgactg gctactcatt cgactgaggc tgccgagcta cccgcttcca cgagcaatgc
     2461 agccgcgtca gcgaccgtgc acggcgttcc ctgtcctcgt tccatcccat actcatcgat
     2521 ccgagaatcc tctggaacaa ggcaggagag cgcaccatct gaatcgtccc acggaacctc
     2581 cacgcgatag cctggcagcg gatcacgcaa gaactcgact ccttccgcga gtacgaccgt
     2641 ctcgctccca tcggccaagt ctgcaaggat ccgcaccgcg cacgaccg
//
LOCUS       DAOSUG010000449         2679 bp    DNA     linear   ENV 10-JUN-2024
DEFINITION  MAG TPA_asm: Micropruina sp. isolate AMR_MDS_2195
            NODE_15375_length_2679_cov_3.882241, whole genome shotgun sequence.
ACCESSION   DAOSUG010000449 DAOSUG010000000
VERSION     DAOSUG010000449.1
DBLINK      BioProject: PRJNA925477
            BioSample: SAMN32864262
            Sequence Read Archive: SRR4236673
KEYWORDS    WGS; ENV; Metagenome Assembled Genome; MAG; Third Party Data; TPA;
            TPA:assembly.
SOURCE      Micropruina sp. (wastewater metagenome)
  ORGANISM  Micropruina sp.
            Bacteria; Bacillati; Actinomycetota; Actinomycetes;
            Propionibacteriales; Nocardioidaceae; Micropruina.
REFERENCE   1  (bases 1 to 2679)
  AUTHORS   Abdulkadir,N., Saraiva,J.P., Zhang,J., Stolte,S., Gillor,O.,
            Harms,H. and Rocha,U.
  TITLE     Genome-centric analyses of 165 metagenomes show that mobile genetic
            elements are crucial for the transmission of antimicrobial
            resistance genes to pathogens in activated sludge and wastewater
  JOURNAL   Microbiol Spectr 12 (3), e0291823 (2024)
   PUBMED   38289113
REFERENCE   2  (bases 1 to 2679)
  AUTHORS   Abdulkadir,N.
  TITLE     Direct Submission
  JOURNAL   Submitted (24-MAR-2023) Environmental Microbiology, Helmholtz
            Center for Environmental Research -UFZ, Permoserstr 15, Leipzig
            04318, Germany
COMMENT     The annotation was added by the NCBI Prokaryotic Genome Annotation
            Pipeline (PGAP). Information about PGAP can be found here:
            https://www.ncbi.nlm.nih.gov/genome/annotation_prok/
            
            ##Genome-Assembly-Data-START##
            Assembly Method        :: SPAdes v. 3.11.1
            Genome Representation  :: Full
            Expected Final Version :: Yes
            Genome Coverage        :: Not Applicable
            Sequencing Technology  :: Illumina MiSeq
            ##Genome-Assembly-Data-END##
            
            ##Genome-Annotation-Data-START##
            Annotation Provider               :: NCBI
            Annotation Date                   :: 05/04/2023 04:57:10
            Annotation Pipeline               :: NCBI Prokaryotic Genome
                                                 Annotation Pipeline (PGAP)
            Annotation Method                 :: Best-placed reference protein
                                                 set; GeneMarkS-2+
            Annotation Software revision      :: 6.5
            Features Annotated                :: Gene; CDS; rRNA; tRNA; ncRNA
            Genes (total)                     :: 2,776
            CDSs (total)                      :: 2,750
            Genes (coding)                    :: 2,714
            CDSs (with protein)               :: 2,714
            Genes (RNA)                       :: 26
            tRNAs                             :: 24
            ncRNAs                            :: 2
            Pseudo Genes (total)              :: 36
            CDSs (without protein)            :: 36
            Pseudo Genes (ambiguous residues) :: 0 of 36
            Pseudo Genes (frameshifted)       :: 2 of 36
            Pseudo Genes (incomplete)         :: 32 of 36
            Pseudo Genes (internal stop)      :: 2 of 36
            ##Genome-Annotation-Data-END##
FEATURES             Location/Qualifiers
     source          1..2679
                     /organism="Micropruina sp."
                     /mol_type="genomic DNA"
                     /submitter_seqid="NODE_15375_length_2679_cov_3.882241"
                     /isolate="AMR_MDS_2195"
                     /isolation_source="Activated sludge of municipal
                     wastewater treatment plants"
                     /db_xref="taxon:2737536"
                     /environmental_sample
                     /geo_loc_name="Canada"
                     /lat_lon="49.25 N 123.1 W"
                     /collection_date="2015-06-16"
                     /metagenome_source="wastewater metagenome"
                     /note="metagenomic"
     gene            164..667
                     /locus_tag="PLF56_12550"
     CDS             164..667
                     /locus_tag="PLF56_12550"
                     /inference="COORDINATES: ab initio
                     prediction:GeneMarkS-2+"
                     /note="Derived by automated computational analysis using
                     gene prediction method: GeneMarkS-2+."
                     /codon_start=1
                     /transl_table=11
                     /product="hypothetical protein"
                     /protein_id="HOQ54428.1"
                     /translation="MTHRVLVLTGGPAAGKSTTALALATTTPRTAFIDVDDVRQLVKN
                     GGAAPWDGEEGASQQLLGVRNATWLASNFVGQGINVTVADVVNPITLALYRELLPDVL
                     VVRLTLGLEEARRRAAQRTVYLTDAEFEELHRQQGDNLQVDHELDVADMGIDEQVAAV
                     MKIWTAG"
     gene            734..1636
                     /locus_tag="PLF56_12555"
     CDS             734..1636
                     /locus_tag="PLF56_12555"
                     /inference="COORDINATES: protein motif:HMM:NF014601.2"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="2-dehydropantoate 2-reductase N-terminal
                     domain-containing protein"
                     /protein_id="HOQ54429.1"
                     /translation="MARVRVLGAGVVGSVYAALLADAGHEVTLTARGSRLVDLRRHGL
                     LVGLDGRILAPDVAVTKSPEVTDCDVLLVAVRGDQLAAALDDIAASPAGWVWMFTNPL
                     ELRSDVEDRLGRDRLVWCFSGVGGGVKDGQVTAHRVGQQPTVVEGGAAGSKFASQLLG
                     AIDPHLRTEPDMHAWLDTHTVFVAAMAALVLEQPLETRSQRVRGAVLLVRAMRQAFDS
                     LEAQGARITPRNLRMIFGRVPLPIAALYWAAQLSRPVVQVSMLPHATATRTTEQRAVA
                     AHALDLAGHGPGLYRQLLAPLAGT"
     gene            1711..2538
                     /locus_tag="PLF56_12560"
     CDS             1711..2538
                     /locus_tag="PLF56_12560"
                     /EC_number="2.7.7.-"
                     /inference="COORDINATES: protein motif:HMM:NF014021.2"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_function: GO:0016779 - nucleotidyltransferase activity
                     [Evidence IEA];
                     GO_function: GO:0046872 - metal ion binding [Evidence
                     IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="nucleotidyltransferase domain-containing
                     protein"
                     /protein_id="HOQ54430.1"
                     /translation="MITDERLQQMADALASVPGIVAIVLGGSRARRTHHEASDVDLGL
                     YYRAEGLDLDALARASRAFSDTGQVEIVGPGGWGPWVNGGGWLTVNGTPVDWILRDLD
                     RVHEQADRARRGEFAFHPQPGHPLGFLDVSYAGEVATCRPLADPDGAVAGLAADLNPY
                     PDALRVAFIDNLWQAGFLVDAARKGLPKDDSAYVMLCCSTALMLCAHAWHANAGSWVT
                     NEKGIIVDVARLDLDTHDFATRANTVLQSSGHMGLSEVVTKTASVVEDTRAALRREP"
BASE COUNT          435 a          954 c          864 g          426 t
ORIGIN      
        1 cacctcaagg atcgcccacc cgccccacaa cagcatttag caacacggcc tagtccccac
       61 cgaggccgtc cacctgatcg ccgaattcct acacaccgag taggacacgc ctttgccgct
      121 gcccatccgc cgccgttgtg aggccgttgg ctacggtgaa tgcatgaccc accgagtgtt
      181 ggtcctcacg ggaggccccg ccgcgggcaa gagcaccacg gcgctggcct tggccaccac
      241 cacccctcgg accgccttca tcgacgtcga tgacgtccgc caactcgtga agaacggagg
      301 cgccgcacct tgggacggtg aggaaggcgc gtcccagcag ctactcgggg tacgcaacgc
      361 gacctggctg gccagcaact tcgtcggcca gggcatcaac gtcacagtcg ccgacgtggt
      421 caatccgatc acgcttgcgc tctatcgaga gctgctgccg gacgtcctcg tggtcaggct
      481 caccctgggt ctggaggagg ccaggcgccg agccgcccag cgaacggtct acctcaccga
      541 cgccgagttc gaggagctgc accgccagca aggtgacaac ctccaggtgg atcatgagct
      601 cgatgtcgcc gacatgggaa ttgatgagca agtggccgca gtgatgaaga tctggaccgc
      661 aggctgaaca tccacacatg ccgccggtgg cgcaccggca cttctgggtc ctcctggcgc
      721 ataggctcgc actatggcgc gtgtgagggt gctcggggca ggtgtcgttg gatcggtcta
      781 cgcggcgctg ctggccgacg ccggtcacga ggtcaccttg accgcacgag gatcgagact
      841 cgtcgacctg cgccgccatg gactgctcgt gggcctcgac ggccggatcc ttgcgcccga
      901 cgtggccgtg acgaagtcac cggaagtgac agactgcgac gtcctgctgg tcgccgttcg
      961 cggtgaccag ttggctgcgg ccctggacga catcgcagcc agcccggctg ggtgggtctg
     1021 gatgttcacc aaccccctcg agctgcgcag tgacgtggag gatcgcctgg gccgcgaccg
     1081 gctggtctgg tgcttctccg gtgtgggcgg cggcgtgaag gacgggcagg tgaccgccca
     1141 ccgggtgggg cagcagccca ccgtggtcga gggcggggca gccggctcca agttcgcgtc
     1201 gcagctgctc ggcgcgatcg acccccacct gcggaccgag ccggacatgc acgcctggtt
     1261 ggacacccac acggtgttcg tggccgccat ggccgccctg gtcctggagc agcccttgga
     1321 gacgcgatca cagcgagtgc gcggcgctgt cctgctggtt cgggccatgc gacaggcctt
     1381 cgactccctc gaagcgcagg gggctcgcat cacaccgcgc aacctgcgga tgatcttcgg
     1441 ccgggtgccc ctgccgatcg ccgccctgta ctgggctgcg cagctgtcac gacccgtcgt
     1501 gcaggtgtcc atgctccccc atgccaccgc gacccgaacc accgaacagc gggccgtggc
     1561 cgctcacgcc ctggacctcg cgggccacgg ccccggcctc taccgccagc tgctcgctcc
     1621 tttggccggc acctgagcgg gtgctcggaa cgcactactt tgccgcgaat ggcatggcag
     1681 gtccacgcgg gctaggcaaa actctggtgt gtgatcaccg acgagcgact ccagcagatg
     1741 gccgatgctc tcgcatcagt tcccggcatc gtggccatcg tcctgggagg cagccgagct
     1801 cgccgcaccc accacgaggc ctcggacgtc gacctcggcc tgtactaccg cgccgaagga
     1861 ctcgacctcg acgccctggc ccgtgcgtca cgcgctttca gtgacaccgg gcaggtcgag
     1921 atcgtcgggc ccggcggctg ggggccctgg gtgaacggag gtggctggct aaccgtcaac
     1981 ggcacccccg tcgactggat cctgcgcgac ctcgaccgcg tgcacgaaca ggccgaccgc
     2041 gcccgccgcg gtgagttcgc cttccacccc caacccggtc accccctggg cttcctcgac
     2101 gtcagctacg ccggcgaggt cgccacgtgc cgtcccctcg ccgaccccga cggcgccgtc
     2161 gccgggctgg cagccgatct caacccgtac cccgatgccc tgcgcgtcgc gttcatcgac
     2221 aacctgtggc aggccggttt cttggtagat gccgcccgca agggcctgcc caaggacgac
     2281 agcgcgtacg tgatgctgtg ctgctcgacc gcgctcatgc tctgcgccca cgcctggcat
     2341 gccaatgccg ggtcgtgggt gaccaacgag aagggcatca tcgtcgacgt ggcgcgactc
     2401 gacctggaca cccacgattt cgcgaccaga gccaacaccg tcttgcagtc gtccgggcac
     2461 atgggactca gtgaagtcgt caccaagact gcatctgtcg tggaggacac tcgcgccgct
     2521 ctccggcgcg aaccgtgagc gacgctcgct catgccgctg ggctcgcgca aggtgggtcc
     2581 acttctggcc gtcagccagg ggccaagtgg ggttgacata gccacccagt gccacactgt
     2641 ggtccgacgt caacatcgag aagatcccgg agggcaact
//
LOCUS       DAOSUG010000450         2672 bp    DNA     linear   ENV 10-JUN-2024
DEFINITION  MAG TPA_asm: Micropruina sp. isolate AMR_MDS_2195
            NODE_15435_length_2672_cov_5.131830, whole genome shotgun sequence.
ACCESSION   DAOSUG010000450 DAOSUG010000000
VERSION     DAOSUG010000450.1
DBLINK      BioProject: PRJNA925477
            BioSample: SAMN32864262
            Sequence Read Archive: SRR4236673
KEYWORDS    WGS; ENV; Metagenome Assembled Genome; MAG; Third Party Data; TPA;
            TPA:assembly.
SOURCE      Micropruina sp. (wastewater metagenome)
  ORGANISM  Micropruina sp.
            Bacteria; Bacillati; Actinomycetota; Actinomycetes;
            Propionibacteriales; Nocardioidaceae; Micropruina.
REFERENCE   1  (bases 1 to 2672)
  AUTHORS   Abdulkadir,N., Saraiva,J.P., Zhang,J., Stolte,S., Gillor,O.,
            Harms,H. and Rocha,U.
  TITLE     Genome-centric analyses of 165 metagenomes show that mobile genetic
            elements are crucial for the transmission of antimicrobial
            resistance genes to pathogens in activated sludge and wastewater
  JOURNAL   Microbiol Spectr 12 (3), e0291823 (2024)
   PUBMED   38289113
REFERENCE   2  (bases 1 to 2672)
  AUTHORS   Abdulkadir,N.
  TITLE     Direct Submission
  JOURNAL   Submitted (24-MAR-2023) Environmental Microbiology, Helmholtz
            Center for Environmental Research -UFZ, Permoserstr 15, Leipzig
            04318, Germany
COMMENT     The annotation was added by the NCBI Prokaryotic Genome Annotation
            Pipeline (PGAP). Information about PGAP can be found here:
            https://www.ncbi.nlm.nih.gov/genome/annotation_prok/
            
            ##Genome-Assembly-Data-START##
            Assembly Method        :: SPAdes v. 3.11.1
            Genome Representation  :: Full
            Expected Final Version :: Yes
            Genome Coverage        :: Not Applicable
            Sequencing Technology  :: Illumina MiSeq
            ##Genome-Assembly-Data-END##
            
            ##Genome-Annotation-Data-START##
            Annotation Provider               :: NCBI
            Annotation Date                   :: 05/04/2023 04:57:10
            Annotation Pipeline               :: NCBI Prokaryotic Genome
                                                 Annotation Pipeline (PGAP)
            Annotation Method                 :: Best-placed reference protein
                                                 set; GeneMarkS-2+
            Annotation Software revision      :: 6.5
            Features Annotated                :: Gene; CDS; rRNA; tRNA; ncRNA
            Genes (total)                     :: 2,776
            CDSs (total)                      :: 2,750
            Genes (coding)                    :: 2,714
            CDSs (with protein)               :: 2,714
            Genes (RNA)                       :: 26
            tRNAs                             :: 24
            ncRNAs                            :: 2
            Pseudo Genes (total)              :: 36
            CDSs (without protein)            :: 36
            Pseudo Genes (ambiguous residues) :: 0 of 36
            Pseudo Genes (frameshifted)       :: 2 of 36
            Pseudo Genes (incomplete)         :: 32 of 36
            Pseudo Genes (internal stop)      :: 2 of 36
            ##Genome-Annotation-Data-END##
FEATURES             Location/Qualifiers
     source          1..2672
                     /organism="Micropruina sp."
                     /mol_type="genomic DNA"
                     /submitter_seqid="NODE_15435_length_2672_cov_5.131830"
                     /isolate="AMR_MDS_2195"
                     /isolation_source="Activated sludge of municipal
                     wastewater treatment plants"
                     /db_xref="taxon:2737536"
                     /environmental_sample
                     /geo_loc_name="Canada"
                     /lat_lon="49.25 N 123.1 W"
                     /collection_date="2015-06-16"
                     /metagenome_source="wastewater metagenome"
                     /note="metagenomic"
     gene            <1..1295
                     /locus_tag="PLF56_12565"
     CDS             <1..1295
                     /locus_tag="PLF56_12565"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_017836497.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=3
                     /transl_table=11
                     /product="Mu transposase C-terminal domain-containing
                     protein"
                     /protein_id="HOQ54431.1"
                     /translation="RWHARYRADGYAGLETASRADTGSRRLPPDLVHLIEGLALSKPR
                     PAIATIHRKVTGICAARRWPVPSYSVVWDIVRTLDPGMVTLALEGAASYRDKHELVLR
                     RQAELPNAMWQSDHTMLDILVVGTDGKPARPWLTTILDDCSRAVCGYTVFLGAPSAMN
                     TALALRQAIWHKTDPAWPMCGLPDVLYVDHGSDFTSDQLAHTAVDLHIRLIHSTVARP
                     QGRGKIERFFGTINTELLATLPGHITEGHPWPTPKLSLAALDSALEAFVATYNDRTHS
                     ELGTSPRSAWIADGWLPRMPETLEDLDRLLLTVAKTRVVRRDGIRFQGLRYVSPTLAG
                     YVGRSVVIRYDPRDITEIRVFDHDEFVCKAVNQEHHDQKVSLKEIQAARNARRRALRA
                     GINERIALVAAPTETPRITEAPTPAPRSALKIYKEDLR"
     gene            1292..2110
                     /locus_tag="PLF56_12570"
     CDS             1292..2110
                     /locus_tag="PLF56_12570"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_010613290.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="AAA family ATPase"
                     /protein_id="HOQ54432.1"
                     /translation="MSQRFIVTKEHRRFTEFADAVRRGHTIGLCFGSAGVGKTLSARR
                     YAHYEKAHDLLTYWGPRSDNDAKIYAALNRSRTVLYTPSVLTTPRTLKDELTQAITRT
                     NMCIEQHLVPPGTATPEAWGWRHGRNYVELIIVDEAERLRPAALELLRDRYDRDDIAL
                     ILIGMPGLEKQFSHYPQFYSRVGFAHQYRPLGQDELLFVLERHWRSLGKTLDPDDFTD
                     AQAIAAIARITRGNFRLLERLFPQIQRVLKINELDTITNDVIEAAQSTLVIGVT"
     gene            2284..>2672
                     /locus_tag="PLF56_12575"
     CDS             2284..>2672
                     /locus_tag="PLF56_12575"
                     /inference="COORDINATES: protein motif:HMM:NF012655.2"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_function: GO:0003677 - DNA binding [Evidence IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="helix-turn-helix domain-containing protein"
                     /protein_id="HOQ54433.1"
                     /translation="MLNVSSTTTLPWSDLKTSARIVAAAFQRFAQSGFTSVTIREIAG
                     EAGVSAALVIHYFGSKDGLRRECDSRVVKFVRDKLRTGAPAAENLQAWLRFDVATSLG
                     FDIPRAVLTVALVVVAGRVVLVALRRAT"
BASE COUNT          514 a         1000 c          770 g          388 t
ORIGIN      
        1 agcgctggca cgcccgctac cgcgccgacg gctacgccgg actggagaca gcctcgcgag
       61 cggacaccgg ctcccgccgc cttccgcccg acctcgtcca cctgatcgag ggcctggcac
      121 tgagcaagcc gcggccggcc atcgccacca tccaccgcaa agtcaccggc atctgcgccg
      181 cccggagatg gccggtcccc tcctactcgg tggtctggga catcgtgcgg accctggatc
      241 ccggcatggt caccctcgcc ctggagggcg cagcgtccta ccgcgacaag cacgagctgg
      301 tgctacgccg gcaagcagag ctgcccaacg cgatgtggca atccgatcac accatgctcg
      361 acatcctggt ggtgggcacc gacggcaagc ccgcacggcc atggctgaca acgatcctcg
      421 acgactgctc ccgggcggtc tgcggctaca cagtcttcct gggcgcaccg tcggcgatga
      481 acaccgccct ggcgctgcgc caagcgatct ggcacaagac cgacccggcc tggccgatgt
      541 gcggcctgcc cgacgtgctc tacgtcgacc acggcagcga cttcaccagt gaccagctcg
      601 cccacaccgc cgtcgacctc cacatccgac tgatccactc caccgtcgcc cgaccccagg
      661 gacggggcaa gatcgagcgg ttcttcggca ccatcaacac cgagctactc gccaccctgc
      721 ccggacacat caccgaaggg cacccctggc cgacaccgaa actgtccctg gccgccctcg
      781 atagcgccct ggaggcgttc gtggccacct acaacgaccg cacgcacagc gagctcggaa
      841 cctccccgcg cagcgcgtgg atcgccgatg gctggctgcc ccgaatgccc gagaccctgg
      901 aagacctcga cagactgctg ttgaccgtcg ccaagacccg cgtcgtgcgc cgcgatggca
      961 tccgcttcca gggcctgcgc tacgtctcgc caactctggc cggctacgtc ggacgctcgg
     1021 tcgtgatccg ctacgacccc cgcgacatca ccgagatccg cgtcttcgat cacgacgaat
     1081 tcgtctgcaa ggccgtcaac caagaacacc acgaccagaa ggtcagcctc aaggagatcc
     1141 aggccgcgcg caacgcccgc cgccgagcgc tgcgagccgg catcaacgaa cgcatcgccc
     1201 tcgtggccgc acctaccgag acaccacgga tcaccgaagc accgactccg gcgccgaggt
     1261 cggcgttgaa gatctacaag gaggacctca ggtgagccag cgcttcatcg tcaccaagga
     1321 gcaccgccgc ttcaccgagt tcgccgacgc cgtgcgccgc gggcacacca tcggtttgtg
     1381 cttcggatca gccggcgtag gaaagacact ctccgcgcgc cgctacgcac actacgagaa
     1441 ggctcatgac ctgctgacct actgggggcc gcgctccgac aacgacgcca agatctacgc
     1501 cgccttgaac cggagccgca ccgtgctcta cacccccagc gtgctgacca ccccgcggac
     1561 cctgaaggac gagctgaccc aagccatcac ccgcaccaac atgtgcatcg agcagcacct
     1621 cgtacctccc ggcacggcca ctcccgaggc ctggggatgg cgacacggca ggaactacgt
     1681 ggagctgatc atcgtcgacg aggccgaacg actgcgtccc gccgctctgg aactgctgcg
     1741 cgatcgctac gaccgcgacg acatcgccct gatcctgatc ggcatgcccg gcctggagaa
     1801 gcagttcagc cactaccccc agttctacag ccgagtcggc ttcgcccacc agtaccggcc
     1861 cctgggacaa gacgaactgc tgttcgtcct cgagcgacac tggcgatccc tcggcaagac
     1921 cctcgacccc gacgacttca ccgacgccca agccatcgca gccatcgcac ggatcacccg
     1981 cggcaacttc cggctcctcg aacgcctctt cccccagatc cagcgggtcc tgaagatcaa
     2041 cgaactcgac accatcacca acgacgtcat cgaagccgca cagagcaccc tggtcatcgg
     2101 tgtcacctga ccccgccacg aagcgaccga agaaccccgc cattcagcag acgaagtcac
     2161 atgagcgatg agagagtcgg ctgactcatg aacggtcgtc tacaagacct atgagcatgt
     2221 tgcgcgggat caggcgcctc ggagctggct ccgccattga cgcgagttga ataccacgac
     2281 agtatgttga acgtgagttc aaccacgacg ttgccttgga gcgatctgaa gacaagcgcc
     2341 cgcatagtgg cagcagcgtt ccagcgcttt gcccagagcg gattcacctc ggtgaccatc
     2401 cgcgagattg ccggggaggc gggggtgagt gccgctctag tgattcacta cttcggcagc
     2461 aaggacggcc tccgtcgaga gtgcgacagt cgcgtggtga agttcgtgcg cgacaaactc
     2521 cggaccggtg ccccggccgc cgagaacctc caggcctggc tgcgcttcga cgtcgcgacg
     2581 tcgctcggct tcgacatccc gcgcgcggtg ctgaccgtcg cgctcgtcgt cgtcgcgggc
     2641 cgcgtcgtgc tggtggcgct gcgacgggcc ac
//
LOCUS       DAOSUG010000451         2657 bp    DNA     linear   ENV 10-JUN-2024
DEFINITION  MAG TPA_asm: Micropruina sp. isolate AMR_MDS_2195
            NODE_15550_length_2657_cov_3.594927, whole genome shotgun sequence.
ACCESSION   DAOSUG010000451 DAOSUG010000000
VERSION     DAOSUG010000451.1
DBLINK      BioProject: PRJNA925477
            BioSample: SAMN32864262
            Sequence Read Archive: SRR4236673
KEYWORDS    WGS; ENV; Metagenome Assembled Genome; MAG; Third Party Data; TPA;
            TPA:assembly.
SOURCE      Micropruina sp. (wastewater metagenome)
  ORGANISM  Micropruina sp.
            Bacteria; Bacillati; Actinomycetota; Actinomycetes;
            Propionibacteriales; Nocardioidaceae; Micropruina.
REFERENCE   1  (bases 1 to 2657)
  AUTHORS   Abdulkadir,N., Saraiva,J.P., Zhang,J., Stolte,S., Gillor,O.,
            Harms,H. and Rocha,U.
  TITLE     Genome-centric analyses of 165 metagenomes show that mobile genetic
            elements are crucial for the transmission of antimicrobial
            resistance genes to pathogens in activated sludge and wastewater
  JOURNAL   Microbiol Spectr 12 (3), e0291823 (2024)
   PUBMED   38289113
REFERENCE   2  (bases 1 to 2657)
  AUTHORS   Abdulkadir,N.
  TITLE     Direct Submission
  JOURNAL   Submitted (24-MAR-2023) Environmental Microbiology, Helmholtz
            Center for Environmental Research -UFZ, Permoserstr 15, Leipzig
            04318, Germany
COMMENT     The annotation was added by the NCBI Prokaryotic Genome Annotation
            Pipeline (PGAP). Information about PGAP can be found here:
            https://www.ncbi.nlm.nih.gov/genome/annotation_prok/
            
            ##Genome-Assembly-Data-START##
            Assembly Method        :: SPAdes v. 3.11.1
            Genome Representation  :: Full
            Expected Final Version :: Yes
            Genome Coverage        :: Not Applicable
            Sequencing Technology  :: Illumina MiSeq
            ##Genome-Assembly-Data-END##
            
            ##Genome-Annotation-Data-START##
            Annotation Provider               :: NCBI
            Annotation Date                   :: 05/04/2023 04:57:10
            Annotation Pipeline               :: NCBI Prokaryotic Genome
                                                 Annotation Pipeline (PGAP)
            Annotation Method                 :: Best-placed reference protein
                                                 set; GeneMarkS-2+
            Annotation Software revision      :: 6.5
            Features Annotated                :: Gene; CDS; rRNA; tRNA; ncRNA
            Genes (total)                     :: 2,776
            CDSs (total)                      :: 2,750
            Genes (coding)                    :: 2,714
            CDSs (with protein)               :: 2,714
            Genes (RNA)                       :: 26
            tRNAs                             :: 24
            ncRNAs                            :: 2
            Pseudo Genes (total)              :: 36
            CDSs (without protein)            :: 36
            Pseudo Genes (ambiguous residues) :: 0 of 36
            Pseudo Genes (frameshifted)       :: 2 of 36
            Pseudo Genes (incomplete)         :: 32 of 36
            Pseudo Genes (internal stop)      :: 2 of 36
            ##Genome-Annotation-Data-END##
FEATURES             Location/Qualifiers
     source          1..2657
                     /organism="Micropruina sp."
                     /mol_type="genomic DNA"
                     /submitter_seqid="NODE_15550_length_2657_cov_3.594927"
                     /isolate="AMR_MDS_2195"
                     /isolation_source="Activated sludge of municipal
                     wastewater treatment plants"
                     /db_xref="taxon:2737536"
                     /environmental_sample
                     /geo_loc_name="Canada"
                     /lat_lon="49.25 N 123.1 W"
                     /collection_date="2015-06-16"
                     /metagenome_source="wastewater metagenome"
                     /note="metagenomic"
     gene            complement(217..879)
                     /locus_tag="PLF56_12580"
     CDS             complement(217..879)
                     /locus_tag="PLF56_12580"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_017936691.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="transglycosylase SLT domain-containing protein"
                     /protein_id="HOQ54434.1"
                     /translation="MQPFVRPVVGVLAALSLATGFTLVPQADTQLPGDQPSVTAVPSL
                     DDRDSQTSRDDTRPALLPAVARALEQRQAALAKTQVEIAVAARTLQGDEFAAARKKAE
                     QDARYAEKVKALGYDPKTTDPRDIAKQMMLSEYEWGEDEFTCVNKIFTQESNWQWNAT
                     NASSGAYGIPQSLPATKMASAGDDWKTNPATQIKWGLKYIKDRYGTPCGAWGFKAVHG
                     WY"
     gene            complement(1056..1301)
                     /locus_tag="PLF56_12585"
     CDS             complement(1056..1301)
                     /locus_tag="PLF56_12585"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_013861667.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="PLDc N-terminal domain-containing protein"
                     /protein_id="HOQ54435.1"
                     /translation="MPRVLPVLILALLVVYCVVEVAQADPDRVRLMPRWLWAVLVIAL
                     PGVGAIAWLILGRPLRRRPPQSRPPAPDDDPDFLRGL"
     gene            1334..2416
                     /locus_tag="PLF56_12590"
     CDS             1334..2416
                     /locus_tag="PLF56_12590"
                     /inference="COORDINATES: protein motif:HMM:NF012711.2"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="AMP-binding protein"
                     /protein_id="HOQ54436.1"
                     /translation="MHTPIATGPAIVAAVAEALNGRPPAIVAPAGSPVPSSWQPDGPP
                     LDDDIAVLIGTSGSTGAPKGVLLARDAITASVTATHRRLGGAGNWVCPLPLHYVAGMM
                     TVARAHLGGGRLSVVASDLTELPLHPGRNYLSLVAAQLHRALDVPGTAASLAGFDAIL
                     IGGSAIPAALLQRGRAAGLSLVPTYGMAETCGGCVYAGVPLDEVSVSVDADERIRLTA
                     PMVFSGYRLDPRATEQVLAGRTLRTSDRGRWHEGRLQVLGRADDVVITGGSNVDLAHV
                     QRVADDELGPGALAVLAVPDERWGVRIVALTDAEWEQAAVRSRLATRLDASAVPREVR
                     RVAGLPRTSTGKIDRHGLRRLWNEGG"
     gene            2417..>2657
                     /locus_tag="PLF56_12595"
     CDS             2417..>2657
                     /locus_tag="PLF56_12595"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_015582117.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="1,4-dihydroxy-2-naphthoate
                     polyprenyltransferase"
                     /protein_id="HOQ54437.1"
                     /translation="MATPEQWWQGARPRTLPAAVAPVLAGSGIAAFEGAFDPVLALLC
                     LLVSLALQVGVNYANDYSDGVRGTDADRVGPMRLVG"
BASE COUNT          362 a          922 c          956 g          417 t
ORIGIN      
        1 gccgggatct cgcccggcgc ctcgttggtc gcccctgaga cgccgtggtc gagcctgttg
       61 agacctggcc cgtctgcgtg gttgcctttg tcgccgcggc gctggagtga tggttcgcga
      121 ctctcggttg cggcagtgtc gaccgtggtg ggtcacgctg gagtgacctt tggggagtcg
      181 aggctactcg caccctcgct cgccaaatcg caggcgtcag taccagccgt gcacggcctt
      241 gaaaccccag gcgccgcagg gggtgccgta acggtccttg atgtacttca gtccccattt
      301 gatctgggtg gcggggttgg tcttccagtc gtcgcccgcg ctcgccatct tggtggcggg
      361 cagcgactgc ggaatgccgt aggcgccgga cgaggcgttg gtcgcgttcc actgccagtt
      421 cgactcctgg gtgaagatct tgttcacgca ggtgaactcg tcctcacccc actcgtactc
      481 gctcagcatc atctgcttgg cgatatcgcg cgggtcggtg gtcttgggat cgtagcccag
      541 ggccttcacc ttctcggcgt agcgggcgtc ctgctcggcc ttcttgcgcg cggccgcgaa
      601 ctcgtcaccc tgcagggtgc gtgcggcgac ggcgatctcg acctgcgtct tcgccaacgc
      661 ggcctgacgc tgttcgagcg cacgagccac ggccggcagc agggccgggc gggtgtcgtc
      721 gcgcgaggtc tgcgagtcgc gatcgtccag ggaaggcacg gcggtcacgg agggctggtc
      781 gcccggaagc tgggtgtcgg cctggggcac gagggtgaac ccggtggcca ggctgagcgc
      841 agccagcacc cccacgacgg gacggacgaa aggctgcacc aaacgagagt gccacaccga
      901 agcgcttcag gaaaatctca ggtggtacat ctgcgcatct gatggccgaa atcgtatcag
      961 cacctggtcg tctggttcga cctgtctacg tgccgaacgt gcgctcggcc ccgtgcggcg
     1021 tgacctgggc gaggcccggt acctcggccg cagcgctaga gcccgcgcag gaagtccggg
     1081 tcgtcgtcgg gggccggcgg acggctctgc ggcggccggc gtcgcagggg gcgtccgaga
     1141 atcagccacg cgatcgcacc cacaccgggc agggcgatga ccagcacagc ccacagccac
     1201 cgcggcatca gccggacgcg gtcggggtcg gcctgggcga cctcgaccac gcagtagacg
     1261 accagcagcg ccagaatcag caccggcagc actcgtggca tacccccaat ctacccggcg
     1321 ttagggtgaa cgggtgcaca ccccgatcgc gaccggtccg gccatcgtgg cggccgtcgc
     1381 ggaggccctg aacgggcgtc caccggcgat cgtcgcgccg gccggttcgc cggtgccgtc
     1441 gtcctggcag ccggacggcc ccccgctcga cgacgacatc gcggtgctga tcggcacgtc
     1501 cgggtcgacg ggggcgccca agggcgtcct gttggcgcgc gacgcgatca ccgcgtcggt
     1561 caccgccacg catcggcgac tgggtggtgc cggcaactgg gtgtgcccgc tgccgctgca
     1621 ctacgtggcc ggcatgatga ccgtcgcgcg cgcacacctg ggcggcggac ggctcagcgt
     1681 ggttgcgtcc gatctgaccg agttgcccct gcacccgggg cgcaactacc tgtcgctggt
     1741 ggccgcccaa ctgcaccgcg cactcgacgt gcccggaacg gctgcgagcc tggccggctt
     1801 cgacgcgatc ctgatcggcg gctcggccat ccccgccgcc ctactgcagc gcggacgagc
     1861 agccgggctc agcctggtgc ccacctacgg catggccgaa acctgtggcg gctgtgtcta
     1921 tgcaggcgta ccgctggacg aggtctcggt gagcgtcgat gccgacgagc ggatccgcct
     1981 caccgcaccg atggtgttct ccggctaccg gctcgacccg cgcgccacgg agcaggtgct
     2041 cgccggacgc accctgcgca cgtccgatcg cggccgctgg cacgaaggca ggctgcaggt
     2101 gctcggccgg gccgacgacg tggtgatcac cggcggcagc aacgtcgacc tggcccacgt
     2161 acagcgcgtc gccgacgacg agctcggacc cggcgcgctg gccgtgctgg ccgtgcccga
     2221 cgagcgctgg ggcgtgcgca tcgtggcgct caccgacgcc gaatgggagc aggcggcggt
     2281 gcgttcgcgg ctggcgaccc gcctggacgc gtccgccgtt ccgcgcgagg tgcgccgcgt
     2341 cgctggcctg ccccgcacgt ccactggcaa gatcgatcgg cacggcctgc gtcggctgtg
     2401 gaacgaaggg ggctgagtgg ccacacccga gcaatggtgg cagggcgcgc ggccacgtac
     2461 cctgccggcc gcggtggctc ccgtgcttgc cggcagtggc atcgccgcgt tcgaaggcgc
     2521 cttcgacccg gtgctcgcac tgctgtgcct gctggtgtcc ctcgcgctgc aggtgggcgt
     2581 caactacgcc aacgactatt cggacggggt gcgcggcacc gacgccgacc gggtcgggcc
     2641 gatgcggttg gtcggct
//
LOCUS       DAOSUG010000452         2650 bp    DNA     linear   ENV 10-JUN-2024
DEFINITION  MAG TPA_asm: Micropruina sp. isolate AMR_MDS_2195
            NODE_15603_length_2650_cov_4.936416, whole genome shotgun sequence.
ACCESSION   DAOSUG010000452 DAOSUG010000000
VERSION     DAOSUG010000452.1
DBLINK      BioProject: PRJNA925477
            BioSample: SAMN32864262
            Sequence Read Archive: SRR4236673
KEYWORDS    WGS; ENV; Metagenome Assembled Genome; MAG; Third Party Data; TPA;
            TPA:assembly.
SOURCE      Micropruina sp. (wastewater metagenome)
  ORGANISM  Micropruina sp.
            Bacteria; Bacillati; Actinomycetota; Actinomycetes;
            Propionibacteriales; Nocardioidaceae; Micropruina.
REFERENCE   1  (bases 1 to 2650)
  AUTHORS   Abdulkadir,N., Saraiva,J.P., Zhang,J., Stolte,S., Gillor,O.,
            Harms,H. and Rocha,U.
  TITLE     Genome-centric analyses of 165 metagenomes show that mobile genetic
            elements are crucial for the transmission of antimicrobial
            resistance genes to pathogens in activated sludge and wastewater
  JOURNAL   Microbiol Spectr 12 (3), e0291823 (2024)
   PUBMED   38289113
REFERENCE   2  (bases 1 to 2650)
  AUTHORS   Abdulkadir,N.
  TITLE     Direct Submission
  JOURNAL   Submitted (24-MAR-2023) Environmental Microbiology, Helmholtz
            Center for Environmental Research -UFZ, Permoserstr 15, Leipzig
            04318, Germany
COMMENT     The annotation was added by the NCBI Prokaryotic Genome Annotation
            Pipeline (PGAP). Information about PGAP can be found here:
            https://www.ncbi.nlm.nih.gov/genome/annotation_prok/
            
            ##Genome-Assembly-Data-START##
            Assembly Method        :: SPAdes v. 3.11.1
            Genome Representation  :: Full
            Expected Final Version :: Yes
            Genome Coverage        :: Not Applicable
            Sequencing Technology  :: Illumina MiSeq
            ##Genome-Assembly-Data-END##
            
            ##Genome-Annotation-Data-START##
            Annotation Provider               :: NCBI
            Annotation Date                   :: 05/04/2023 04:57:10
            Annotation Pipeline               :: NCBI Prokaryotic Genome
                                                 Annotation Pipeline (PGAP)
            Annotation Method                 :: Best-placed reference protein
                                                 set; GeneMarkS-2+
            Annotation Software revision      :: 6.5
            Features Annotated                :: Gene; CDS; rRNA; tRNA; ncRNA
            Genes (total)                     :: 2,776
            CDSs (total)                      :: 2,750
            Genes (coding)                    :: 2,714
            CDSs (with protein)               :: 2,714
            Genes (RNA)                       :: 26
            tRNAs                             :: 24
            ncRNAs                            :: 2
            Pseudo Genes (total)              :: 36
            CDSs (without protein)            :: 36
            Pseudo Genes (ambiguous residues) :: 0 of 36
            Pseudo Genes (frameshifted)       :: 2 of 36
            Pseudo Genes (incomplete)         :: 32 of 36
            Pseudo Genes (internal stop)      :: 2 of 36
            ##Genome-Annotation-Data-END##
FEATURES             Location/Qualifiers
     source          1..2650
                     /organism="Micropruina sp."
                     /mol_type="genomic DNA"
                     /submitter_seqid="NODE_15603_length_2650_cov_4.936416"
                     /isolate="AMR_MDS_2195"
                     /isolation_source="Activated sludge of municipal
                     wastewater treatment plants"
                     /db_xref="taxon:2737536"
                     /environmental_sample
                     /geo_loc_name="Canada"
                     /lat_lon="49.25 N 123.1 W"
                     /collection_date="2015-06-16"
                     /metagenome_source="wastewater metagenome"
                     /note="metagenomic"
     gene            complement(<1..1074)
                     /locus_tag="PLF56_12600"
     CDS             complement(<1..1074)
                     /locus_tag="PLF56_12600"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_032491516.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_function: GO:0004803 - transposase activity [Evidence
                     IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="ISL3 family transposase"
                     /protein_id="HOQ54438.1"
                     /translation="MGAIVEDVDVDDPDLIVVRVRPVKRWAGRCGRCRRRSPWYDRGA
                     GRRQWRTVDVGLSRAVVEAEAPRVSCREHGVVVAHVPWARHGAGHTLVFDRTVAWLAT
                     QTSKTATTQLLRISWRTVGAIVSRYWADHERLQDRLRGVRRLGIDEISYKRGHRFLTV
                     VVDHDTGHLLWAAPGRDSATLHKFFDLLGPDRCALLTHVSADGATYISTVVKQRCPNA
                     VRCADPFHVVGWATDALDKLRRIVISTARRQARRAAVDRIAGGSSPVVTAGQAGADLV
                     KGMAGARSALLRNSEDLTENQQVKLAWIALTDPDLHRAYLLKEGLRLIFKLPVEQAAT
                     ALENWITWARTAGIGLFSSLAKRI"
     gene            1342..2646
                     /locus_tag="PLF56_12605"
     CDS             1342..2646
                     /locus_tag="PLF56_12605"
                     /inference="COORDINATES: protein motif:HMM:NF033550.5"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_function: GO:0004803 - transposase activity [Evidence
                     IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="ISL3 family transposase"
                     /protein_id="HOQ54439.1"
                     /translation="MSDDVTGVFVLPGFRVVSSDVLDDEWHLLVETRREPTGCPTCGA
                     VARVKDRRTVTVRDLPAGGVPVVLRWCKRIFECRYGLCEKKTWTEQHDAIAPRVVLTD
                     RAQQWAFEQVGHHDRAVSRVAAQLGVSWHTIMTQVVDRGTPLVEDPDRLAGVSAVGVD
                     ETSFLRATGTRHTQYATGVADLTPGRPPRLLDVVPGRSGRVLGDWLTDRDEAWRSAVL
                     TASLDPFRGYATALSAHLPAATRVLDAFHIVKIVLLAVDQVRRRVQQDTTGHRGRAGD
                     PLYRVRRILRRRYDRLTDRQLVRLRAALTDADTHEEITAAWLVAQNVMQAYANPDRAA
                     GRAAAEQVITLAKTCPVPEIARFGRTLVAWRTEYLARFDNPALSNGPTENLNLKIKNT
                     KRIARGYRSFANYRLRLLLNHGLIRQDQQPARIRPHRPRLIA"
BASE COUNT          437 a          971 c          855 g          387 t
ORIGIN      
        1 gatccgtttc gccaggctgc tgaacaggcc gatgccggcg gtccgcgccc aggtgatcca
       61 gttctccagg gcggtggcgg cctgctcgac cggcagtttg aagatcagtc gaaggccttc
      121 cttgagcaga tacgcgcggt gcaggtcggg gtcggtcagc gcgatccagg ccagtttgac
      181 ctgctgattc tcggtgaggt cctccgagtt tcgcagcaac gccgaccggg ccccggccat
      241 gcccttgacc aggtcagcgc ctgcttgccc ggcggtgacg accgggctcg agccgcccgc
      301 gatgcggtcg acggcggccc ggcgggcctg ccgacgggcc gtgctgatca cgatgcgtcg
      361 tagcttgtcc aaggcgtcgg tagcccaccc aacaacatgg aacgggtcag cacaccggac
      421 cgcgttcggg catcgctgct tgacgaccgt gctgatgtaa gtggcgccgt cggccgagac
      481 atgggtcagc agagcgcacc gatccgggcc cagaagatcg aagaacttgt gcaacgttgc
      541 cgagtcccgg ccgggtgccg cccacaacaa gtgaccggtg tcgtggtcca ccacgaccgt
      601 gaggaaccgg tggccacgct tgtaggagat ctcatcgatc ccgaggcggc ggaccccgcg
      661 taaccggtcc tgcaaccgtt catgatcggc ccagtaccga gacacgatcg ccccgacagt
      721 gcgccaactg atccgcaaca gctgggtcgt ggccgtcttc gaggtctgag tggccaacca
      781 cgccacggtc cggtcgaaca ccagggtgtg gcccgcgccg tgcctggccc agggcacatg
      841 cgcaacgacc acaccatgct cacggcacga cacccgaggg gcctcagcct ccaccaccgc
      901 ccgggacaac cccacgtcca ccgtccgcca ctgccgacgg ccggcgcccc ggtcgtacca
      961 cggcgaccgg cgtcgacacc ggccacaccg ccccgcccac ctcttcactg gccggacccg
     1021 caccacgatc aagtccggat catcgacgtc gacgtcctcg acgatcgccc ccatcaggcc
     1081 caacgcccga tgccataccc tgtgtccgcg cacgccgctc tccgcccacc gttccgggac
     1141 cttcagacag ccccagaacc taggcgcggg agcggcgtgt tgccattccc caccccaaca
     1201 aacgggctct tcgctttgaa gctgggtggg ggtttgatcc cggggcgaca cgccggggct
     1261 gagatgcgct gatgtggggc ctggggtcct cacgatccgt gttcaccgcc aagtgaatgc
     1321 gacccgtgag gaaggcccca ggtgagcgat gacgttaccg gtgtgttcgt gctgcccgga
     1381 ttccgggtgg tgtccagcga cgtgctcgac gacgaatggc acctgcttgt ggaaacgcgg
     1441 cgggagccga cgggctgccc gacctgcggc gcggtcgccc gagtcaagga ccggcgcacg
     1501 gtgaccgtgc gggacctgcc ggccggcggt gtgccggtcg ttctccgttg gtgcaagcgg
     1561 attttcgagt gtcgatacgg gttgtgcgag aagaagacct ggaccgagca acacgacgcg
     1621 atcgccccgc gtgtggtgct caccgaccgt gcgcagcagt gggcgttcga gcaggtcggc
     1681 caccacgacc gggccgtgtc ccgggtcgcg gcccaactcg gcgtgtcctg gcacacgatc
     1741 atgacccagg tcgttgaccg cggcaccccg ctggtcgagg acccggaccg gctggccggg
     1801 gtgagcgcgg tcggggtcga cgagacctcg ttcctgcggg ccaccggcac ccggcacacc
     1861 cagtacgcca ccggcgtcgc cgacctgacc ccgggccgcc caccgcggct gctggacgtc
     1921 gtaccgggcc gctccggccg agtcctgggc gactggctga ccgaccgcga cgaggcctgg
     1981 cggtccgccg ttctgaccgc gtcgctggac ccgttccgcg gttatgccac cgcgctctcg
     2041 gcgcatctac ctgcggcgac gcgcgtgctg gacgccttcc acatcgtcaa gatcgtgctt
     2101 ctcgcggtcg accaggtccg ccgccgcgtg cagcaggaca ccaccgggca ccgcggccgg
     2161 gccggcgacc cgctgtaccg ggtacgacgg atcctgcggc gccgctacga ccggctcacc
     2221 gaccggcaac tggtccgact gcgggccgca ctgaccgacg cggacacgca cgaggagatc
     2281 accgccgcct ggctcgtcgc gcagaacgtc atgcaggcct acgccaaccc cgaccgggcc
     2341 gccggacgcg ccgcggccga gcaggtcatc accctggcca agacctgccc ggttcccgag
     2401 atcgcccgct tcgggcgcac tctggtcgcg tggcggaccg agtacctggc ccggttcgac
     2461 aaccccgccc tatccaacgg acccaccgag aacctcaacc tgaagatcaa gaacaccaaa
     2521 cggatcgccc gcggctaccg atcgttcgcc aactaccgtc tgcgactact tctcaaccac
     2581 ggcctcatca ggcaagatca acaaccggca cggatccgac cacaccgacc caggttgatt
     2641 gcgtagagcc
//
LOCUS       DAOSUG010000453         2646 bp    DNA     linear   ENV 10-JUN-2024
DEFINITION  MAG TPA_asm: Micropruina sp. isolate AMR_MDS_2195
            NODE_15631_length_2646_cov_4.238132, whole genome shotgun sequence.
ACCESSION   DAOSUG010000453 DAOSUG010000000
VERSION     DAOSUG010000453.1
DBLINK      BioProject: PRJNA925477
            BioSample: SAMN32864262
            Sequence Read Archive: SRR4236673
KEYWORDS    WGS; ENV; Metagenome Assembled Genome; MAG; Third Party Data; TPA;
            TPA:assembly.
SOURCE      Micropruina sp. (wastewater metagenome)
  ORGANISM  Micropruina sp.
            Bacteria; Bacillati; Actinomycetota; Actinomycetes;
            Propionibacteriales; Nocardioidaceae; Micropruina.
REFERENCE   1  (bases 1 to 2646)
  AUTHORS   Abdulkadir,N., Saraiva,J.P., Zhang,J., Stolte,S., Gillor,O.,
            Harms,H. and Rocha,U.
  TITLE     Genome-centric analyses of 165 metagenomes show that mobile genetic
            elements are crucial for the transmission of antimicrobial
            resistance genes to pathogens in activated sludge and wastewater
  JOURNAL   Microbiol Spectr 12 (3), e0291823 (2024)
   PUBMED   38289113
REFERENCE   2  (bases 1 to 2646)
  AUTHORS   Abdulkadir,N.
  TITLE     Direct Submission
  JOURNAL   Submitted (24-MAR-2023) Environmental Microbiology, Helmholtz
            Center for Environmental Research -UFZ, Permoserstr 15, Leipzig
            04318, Germany
COMMENT     The annotation was added by the NCBI Prokaryotic Genome Annotation
            Pipeline (PGAP). Information about PGAP can be found here:
            https://www.ncbi.nlm.nih.gov/genome/annotation_prok/
            
            ##Genome-Assembly-Data-START##
            Assembly Method        :: SPAdes v. 3.11.1
            Genome Representation  :: Full
            Expected Final Version :: Yes
            Genome Coverage        :: Not Applicable
            Sequencing Technology  :: Illumina MiSeq
            ##Genome-Assembly-Data-END##
            
            ##Genome-Annotation-Data-START##
            Annotation Provider               :: NCBI
            Annotation Date                   :: 05/04/2023 04:57:10
            Annotation Pipeline               :: NCBI Prokaryotic Genome
                                                 Annotation Pipeline (PGAP)
            Annotation Method                 :: Best-placed reference protein
                                                 set; GeneMarkS-2+
            Annotation Software revision      :: 6.5
            Features Annotated                :: Gene; CDS; rRNA; tRNA; ncRNA
            Genes (total)                     :: 2,776
            CDSs (total)                      :: 2,750
            Genes (coding)                    :: 2,714
            CDSs (with protein)               :: 2,714
            Genes (RNA)                       :: 26
            tRNAs                             :: 24
            ncRNAs                            :: 2
            Pseudo Genes (total)              :: 36
            CDSs (without protein)            :: 36
            Pseudo Genes (ambiguous residues) :: 0 of 36
            Pseudo Genes (frameshifted)       :: 2 of 36
            Pseudo Genes (incomplete)         :: 32 of 36
            Pseudo Genes (internal stop)      :: 2 of 36
            ##Genome-Annotation-Data-END##
FEATURES             Location/Qualifiers
     source          1..2646
                     /organism="Micropruina sp."
                     /mol_type="genomic DNA"
                     /submitter_seqid="NODE_15631_length_2646_cov_4.238132"
                     /isolate="AMR_MDS_2195"
                     /isolation_source="Activated sludge of municipal
                     wastewater treatment plants"
                     /db_xref="taxon:2737536"
                     /environmental_sample
                     /geo_loc_name="Canada"
                     /lat_lon="49.25 N 123.1 W"
                     /collection_date="2015-06-16"
                     /metagenome_source="wastewater metagenome"
                     /note="metagenomic"
     gene            <1..198
                     /locus_tag="PLF56_12610"
     CDS             <1..198
                     /locus_tag="PLF56_12610"
                     /EC_number="4.2.3.1"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_015070503.1"
                     /note="catalyzes the formation of L-threonine from
                     O-phospho-L-homoserine; Derived by automated computational
                     analysis using gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="threonine synthase"
                     /protein_id="HOQ54440.1"
                     /translation="APMIVLETALPVKFAATIVEALGREPERPAAFEGIENLPRHVTD
                     IPADAELLKTVIQQRLAALRG"
     gene            complement(334..1032)
                     /locus_tag="PLF56_12615"
     CDS             complement(334..1032)
                     /locus_tag="PLF56_12615"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_020385868.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="M50 family metallopeptidase"
                     /protein_id="HOQ54441.1"
                     /translation="MLRELWLRATTVQPPLAASSVLLVGVAVLLITWSPIGYRLVRHL
                     ITLVHEAGHALVATLAGRSLRGIRLHSDTSGLTISKGRPTGPGVVFTLMGGYPAPSVL
                     GLAGAFVLSRGYAAGLLWGLVLTCAVMLLLIRNLYGLWVVLATGVGVAALSWLAPSAW
                     LVWVATALVWTLLLAAPRSVVELQVQRRRGRGAGSDVAQLAALTRVPAVLWVGLFLVL
                     CLAALGGAGWLLVR"
     gene            complement(1163..1999)
                     /locus_tag="PLF56_12620"
     CDS             complement(1163..1999)
                     /locus_tag="PLF56_12620"
                     /EC_number="3.-.-.-"
                     /inference="COORDINATES: protein motif:HMM:TIGR00099.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_function: GO:0016787 - hydrolase activity [Evidence
                     IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="HAD family hydrolase"
                     /protein_id="HOQ54442.1"
                     /translation="MTKRRPRVLLLDIDGTVVNYQLEVPAGAVAALRAARAQGHLVYL
                     CTGRSRAEIYPHLWDLGVDGLIGGNGSYIEDGDLVVFHQVLDPAVVQRAVDWMIERDL
                     GFYLECNSGLYGSDNLPEKAAAILGGPTPENIAFVRNGFPDMIYGVHEGRDDVNKISF
                     VLEEGLDLDALARDYAGQASIDTWSMTGKGPEFGEFGQLGIHKGGAVRRLAEHLGVSR
                     DDLIGFGDARSDLELFAACGTSVAMGNAPDEVRTAASLVTDHVDENGLANAFVRLGLI
                     DG"
     gene            complement(2009..>2646)
                     /locus_tag="PLF56_12625"
     CDS             complement(2009..>2646)
                     /locus_tag="PLF56_12625"
                     /inference="COORDINATES: protein motif:HMM:NF013591.2"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_function: GO:0004222 - metalloendopeptidase activity
                     [Evidence IEA];
                     GO_process: GO:0006508 - proteolysis [Evidence IEA]"
                     /codon_start=3
                     /transl_table=11
                     /product="M3 family metallopeptidase"
                     /protein_id="HOQ54443.1"
                     /translation="GHALHGLLADSRYPSQSGAQTPRDFVEFPSQVNEMWSLDPTLLP
                     RYAVHHETGEPIPSEWIDDLLAAELFNQGWSTTELLAASILDQAWHSTPIDHLPTDVD
                     GVLDFERQALATAGVDYPLAPTRYRSTYFRHIFEGGYAAGYYSYIWSEVLDADAGAWF
                     LANGGLTRANGQRYRDTLLAPGGSVDVMSTYRAFRGQDPDITHLLARRGLN"
BASE COUNT          461 a          957 c          873 g          355 t
ORIGIN      
        1 gctccgatga tcgtgctcga gaccgccctg ccggtgaagt tcgcggcgac catcgtcgag
       61 gcgctgggtc gtgagcccga gcgtccggcg gcgttcgagg gcattgagaa cctgccgcgc
      121 cacgtgaccg acattcccgc cgacgccgag ctgctgaaga cggtgatcca gcagcggctg
      181 gcggccctgc gcggctgagg caacgccctc aatcgctggt cacgtgggga gccgccgaaa
      241 cccgcgcgcc agcgcgacgg agcgcggcgt gtcggggcca cctggcgcca ctggcgcgca
      301 cctttcgttt ttccggcttc cagccaccgg ccatcagcgc accaacagcc aaccggcacc
      361 gcccaaggcg gccaaacaga gcacgagaaa caagcccacc cacagcaccg ccggcaccct
      421 ggtcagggcg gccagctggg cgacgtccga tccggcgccc cggccacggc gccgctgcac
      481 ctgcagttcc accactgagc ggggggcggc cagcagcagc gtccacacca gggcggtcgc
      541 cacccacacc aaccacgcgg acggtgccag ccacgacagg gccgccactc ccacgcccgt
      601 cgccagcacc acccacaggc cgtacagatt gcggatcaac agcagcatca cagcgcaggt
      661 caacaccagg ccccacagca ggccggcggc gtagccgcgg ctcagtacga aagcgcccgc
      721 cagccccagc acggacggcg cggggtaacc ccccatcagg gtgaacacca ccccaggacc
      781 ggtcggacgg cccttgctga tggtcagccc cgaggtgtcc gagtgcagcc ggatgccgcg
      841 caggctgcgt ccggccaggg ttgccaccag ggcgtggccg gcctcgtgca ccagcgtgat
      901 caggtgccgg accagccggt agccgatcgg agaccaggtg atcagcagca ccgccacacc
      961 caccagcagc acggacgaag ccgccagcgg cggttgcaca gtggtggccc gcagccacag
     1021 ctcacgcagc acggcaccca ccgtagacgg acgaagctgt gaaccggacg cacgcgacga
     1081 ctccggccgg acgatggaac atctgactca acagccccat cgatcgggcg atcaggcacg
     1141 ccgtcgcggc cccggcgtcc cttcagccgt cgatcaaccc gagccggacg aatgcgttgg
     1201 ccaggccgtt ctcgtccacg tgatcggtga ccaggctcgc ggcggtcctc acctcgtccg
     1261 gcgcgttgcc catggccacg gacgtaccgc aggcggcgaa cagctccaga tcgctgcggg
     1321 cgtcgccgaa accgatcagg tcatcacggc tcacacccag gtgctcggcc aaccgccgga
     1381 ccgccccgcc cttgtggatg ccgagttgac cgaactcgcc gaactcgggg cccttgccgg
     1441 tcatgctcca ggtgtcgatg gacgcctggc cggcgtagtc gcgcgccagg gcgtccaggt
     1501 cgaggccctc ctcgagcacg aagctgatct tgttgacgtc gtcgcggcct tcgtgaacgc
     1561 cgtagatcat gtcgggaaag ccgttgcgca cgaaggcgat gttctcgggg gtcggaccac
     1621 ccaggatcgc cgccgccttc tcgggcaggt tgtcgcttcc gtacaggccc gagttgcatt
     1681 ccaggtagaa acccaggtcg cgctcgatca tccagtcgac cgcacgctgc accacggccg
     1741 ggtcgagcac ctggtgaaac acgaccaggt cgccgtcctc gatgtagctg ccgttgccgc
     1801 cgatcagccc gtcgacgccc agatcccaca gatgggggta gatctcggcg cggctgcgtc
     1861 cggtgcagag gtagaccaga tgtccttgcg cccgagccgc ccgcagcgcc gcgaccgcgc
     1921 cggccggtac ctcgagctgg tagttgacca ccgtgccgtc gatgtcgagc agcagcacgc
     1981 ggggacgccg tttcgtcatc gccagtgctc agttcagccc gcggcgggcc agcagatggg
     2041 tgatgtccgg atcctgacca cgaaacgccc gataggtgct catcacgtcg accgaaccgc
     2101 cgggggccag cagtgtgtcg cggtaccgct gcccgttcgc cctggtcagg ccaccattgg
     2161 ccagaaacca ggcgcccgcg tcggcgtcca gcacctcgga ccagatgtag ctgtagtacc
     2221 cggccgcgta gccgccctcg aagatgtgcc gaaagtaggt ggagcggtac cgggtgggcg
     2281 ccagcgggta gtcgacgccc gcggtggcca gcgcctgccg ctcgaaatcc agcaccccgt
     2341 cgacgtcggt cggcaggtgg tcgatcggcg tcgaatgcca ggcctgatcg agaatcgagg
     2401 cagccagcag ttcggtggtc gaccagccct ggttgaacag ctcggcggcc agcaggtcgt
     2461 cgatccattc cgacggaatc ggctcaccag tctcgtggtg caccgcgtag cgcggcagca
     2521 gggtcggatc gagcgaccac atctcgttca cctgcgaggg aaactcgacg aagtcgcgcg
     2581 gcgtctgcgc ccccgactgg gacgggtagc ggctgtcggc gagcagcccg tgcagcgcgt
     2641 ggccga
//
LOCUS       DAOSUG010000454         2642 bp    DNA     linear   ENV 10-JUN-2024
DEFINITION  MAG TPA_asm: Micropruina sp. isolate AMR_MDS_2195
            NODE_15670_length_2642_cov_4.070352, whole genome shotgun sequence.
ACCESSION   DAOSUG010000454 DAOSUG010000000
VERSION     DAOSUG010000454.1
DBLINK      BioProject: PRJNA925477
            BioSample: SAMN32864262
            Sequence Read Archive: SRR4236673
KEYWORDS    WGS; ENV; Metagenome Assembled Genome; MAG; Third Party Data; TPA;
            TPA:assembly.
SOURCE      Micropruina sp. (wastewater metagenome)
  ORGANISM  Micropruina sp.
            Bacteria; Bacillati; Actinomycetota; Actinomycetes;
            Propionibacteriales; Nocardioidaceae; Micropruina.
REFERENCE   1  (bases 1 to 2642)
  AUTHORS   Abdulkadir,N., Saraiva,J.P., Zhang,J., Stolte,S., Gillor,O.,
            Harms,H. and Rocha,U.
  TITLE     Genome-centric analyses of 165 metagenomes show that mobile genetic
            elements are crucial for the transmission of antimicrobial
            resistance genes to pathogens in activated sludge and wastewater
  JOURNAL   Microbiol Spectr 12 (3), e0291823 (2024)
   PUBMED   38289113
REFERENCE   2  (bases 1 to 2642)
  AUTHORS   Abdulkadir,N.
  TITLE     Direct Submission
  JOURNAL   Submitted (24-MAR-2023) Environmental Microbiology, Helmholtz
            Center for Environmental Research -UFZ, Permoserstr 15, Leipzig
            04318, Germany
COMMENT     The annotation was added by the NCBI Prokaryotic Genome Annotation
            Pipeline (PGAP). Information about PGAP can be found here:
            https://www.ncbi.nlm.nih.gov/genome/annotation_prok/
            
            ##Genome-Assembly-Data-START##
            Assembly Method        :: SPAdes v. 3.11.1
            Genome Representation  :: Full
            Expected Final Version :: Yes
            Genome Coverage        :: Not Applicable
            Sequencing Technology  :: Illumina MiSeq
            ##Genome-Assembly-Data-END##
            
            ##Genome-Annotation-Data-START##
            Annotation Provider               :: NCBI
            Annotation Date                   :: 05/04/2023 04:57:10
            Annotation Pipeline               :: NCBI Prokaryotic Genome
                                                 Annotation Pipeline (PGAP)
            Annotation Method                 :: Best-placed reference protein
                                                 set; GeneMarkS-2+
            Annotation Software revision      :: 6.5
            Features Annotated                :: Gene; CDS; rRNA; tRNA; ncRNA
            Genes (total)                     :: 2,776
            CDSs (total)                      :: 2,750
            Genes (coding)                    :: 2,714
            CDSs (with protein)               :: 2,714
            Genes (RNA)                       :: 26
            tRNAs                             :: 24
            ncRNAs                            :: 2
            Pseudo Genes (total)              :: 36
            CDSs (without protein)            :: 36
            Pseudo Genes (ambiguous residues) :: 0 of 36
            Pseudo Genes (frameshifted)       :: 2 of 36
            Pseudo Genes (incomplete)         :: 32 of 36
            Pseudo Genes (internal stop)      :: 2 of 36
            ##Genome-Annotation-Data-END##
FEATURES             Location/Qualifiers
     source          1..2642
                     /organism="Micropruina sp."
                     /mol_type="genomic DNA"
                     /submitter_seqid="NODE_15670_length_2642_cov_4.070352"
                     /isolate="AMR_MDS_2195"
                     /isolation_source="Activated sludge of municipal
                     wastewater treatment plants"
                     /db_xref="taxon:2737536"
                     /environmental_sample
                     /geo_loc_name="Canada"
                     /lat_lon="49.25 N 123.1 W"
                     /collection_date="2015-06-16"
                     /metagenome_source="wastewater metagenome"
                     /note="metagenomic"
     gene            complement(<1..208)
                     /gene="rpmE"
                     /locus_tag="PLF56_12630"
     CDS             complement(<1..208)
                     /gene="rpmE"
                     /locus_tag="PLF56_12630"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_013863912.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_component: GO:0000315 - organellar large ribosomal
                     subunit [Evidence IEA];
                     GO_component: GO:0022625 - cytosolic large ribosomal
                     subunit [Evidence IEA];
                     GO_function: GO:0003735 - structural constituent of
                     ribosome [Evidence IEA];
                     GO_process: GO:0006412 - translation [Evidence IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="50S ribosomal protein L31"
                     /protein_id="HOQ54444.1"
                     /translation="MKQGIHPDYNVTEVTCTCGNTFTTRSTAKNGVIHAEVCAACHPF
                     YTGKQKILDTGGRVARFERRYAKQP"
     gene            460..2142
                     /locus_tag="PLF56_12635"
     CDS             460..2142
                     /locus_tag="PLF56_12635"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_002527553.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_function: GO:0005524 - ATP binding [Evidence IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="ATP-binding cassette domain-containing protein"
                     /protein_id="HOQ54445.1"
                     /translation="MGHVELSGVGYDLADGRPLLTDVSFRVGDGAIVALVGANGSGKT
                     TLLRIVAGDLTPTEGSISRSGGLGVMRQFIGSIRDHSTVRDLLFSLSPTRIADASAAV
                     DRLELQLMDDDSERVQLAYAHALTEYADAGGYDQEVVFDACCAAALGIGYDAAKWREV
                     NTLSGGEQKRLALEVLLRGPDEVLLLDEPDNYLDVPGKRWLEDQLRATQKTVLLVSHD
                     RALLARAATRIAAIELGAAGNTVWVHGRGFATFAKARQERFARFEELRRRWDEQHAKL
                     RELMLMYKNKAAFNSDMASRYQAAVTRLAKFEEAGPPQAVPLRQQVSMRLSGGRTGRR
                     AVVVEQLELTGLMKPFDAEIWFGDRVAVLGSNGSGKSHFLRLLARGGSSPEPGNTSVD
                     HALIDPVRFTGVARLGARVRPGLIAQTHAHPELIGRTLIEILHRGDDHRDGMGREAAA
                     RVLDRYELAKAAEQRYDTLSGGQQARLQILLLQLAGATLLLLDEPTDNLDLESAEALE
                     AGLDAFEGTVIAVTHDRWFARGFDRFVVFGADGSVYEADEPVWDEGRVARSR"
     gene            complement(2279..>2642)
                     /locus_tag="PLF56_12640"
     CDS             complement(2279..>2642)
                     /locus_tag="PLF56_12640"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_013865497.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=2
                     /transl_table=11
                     /product="AAA family ATPase"
                     /protein_id="HOQ54446.1"
                     /translation="TAELARAATETLEREGTVGVIVADDAVAAAIDALDDAGLEFGVL
                     GEEPAGQDFDHRLDVVPATLAKGLEFDQVVLFEPAAIVAGEPDRVTGLRRLYVCLTRA
                     VSGLRIVHEQPLPPELDG"
BASE COUNT          385 a          906 c          922 g          429 t
ORIGIN      
        1 ccggctgctt ggcgtagcgg cgctcgaacc gggccacgcg gccgccggtg tcgaggatct
       61 tctgcttgcc ggtgtagaac gggtggcagg ccgcgcagac ctcggcgtgg atcacgccgt
      121 tcttggcggt gctgcgggtg gtgaacgtgt tgccgcaggt gcaggtcacc tcggtcacgt
      181 tgtagtcggg atggattccc tgcttcatgg tgagatctcc ttagttgtgc cgggtcgccc
      241 tgatctgaag cgggtcgaaa ccccatggcg tgaaccggca acgagacgtc cattctgccc
      301 gatcgggctg gacatgagca aaacgcggac gggtggtggt tcattcccgg cccgtcatta
      361 ctgcccgtca tcccggcgac cagcgggatc tcacccccct gcccggagtc tcagcccacc
      421 ccgggatcac gaccggccct ttcagttagg gtcgtcgcca tgggccacgt ggaactgtcg
      481 ggcgtcggct acgacctggc cgacggacgt ccgctgctca ccgacgtgtc cttccgggtg
      541 ggcgacggcg cgatcgtcgc cctggtcggc gccaacggct cgggcaagac caccctgctg
      601 cggatcgtcg ccggcgacct cacccctacc gagggcagca tctcgcgctc cggcgggctg
      661 ggtgtgatgc ggcagttcat cggcagcatt cgcgaccaca gcaccgtccg cgacctgctg
      721 ttctcgctct ctcccacccg gatcgcggac gcctcggccg cggtggatcg gctcgaactg
      781 caactgatgg acgacgactc cgagcgggtc cagttggcgt acgcgcacgc gctcaccgag
      841 tacgccgacg ccggcggcta cgaccaggag gtggtcttcg acgcctgctg tgcggccgcg
      901 ctgggcatcg gctacgacgc ggcgaagtgg cgtgaggtca acaccctctc cggcggcgag
      961 cagaagcggc tcgccctgga ggtgctgctg cgcggcccgg acgaggtgct gctgctcgac
     1021 gaacccgaca actacctcga cgtgcccggc aaacggtggc tcgaggatca gttgcgggcc
     1081 actcagaaga ccgtgctgct ggtcagtcac gaccgcgcac tgctggcccg ggcggccacc
     1141 cggatcgccg cgatcgaact cggggccgcg ggtaacacgg tgtgggtgca cggtcgcgga
     1201 ttcgccacct tcgccaaggc gcgacaggag cgcttcgccc ggttcgagga actacgcaga
     1261 cgttgggacg agcagcacgc caagctgcgc gagctgatgc tgatgtacaa gaacaaggcg
     1321 gccttcaact ccgacatggc gtcgcgttat caggccgcgg tcacccgcct ggccaagttc
     1381 gaggaggccg ggccaccgca ggcggtccca ctgcgccagc aggtgtcgat gcgcctgtcc
     1441 ggcggacgca ccggtcgccg cgcggtggtc gtcgagcagt tggagctgac cgggctgatg
     1501 aagccgttcg acgccgagat ctggttcggg gaccgggtgg cggtgctggg ctcgaacggc
     1561 tccggcaagt cgcacttcct tcggctgctg gcccgcggcg ggtcatcgcc ggagcctggg
     1621 aacacctcgg tcgaccacgc cctgatcgac cccgtccggt tcaccggcgt ggcccggttg
     1681 ggggcacggg tgcgtccggg cctgattgcg cagacccacg cccaccccga gttgatcggt
     1741 cgcaccctga tcgagatcct gcaccggggt gacgaccatc gcgacggcat gggacgcgag
     1801 gccgccgccc gggttcttga ccgctacgag ctggccaagg ccgccgagca gcgctatgac
     1861 accttgtcgg gcggccagca ggcacggctg cagatcctgc tgctgcaact ggccggcgcc
     1921 accctgttgc tgctggacga gcccactgac aacctcgacc tggagtcggc cgaggcgctg
     1981 gaggccggcc tggacgcctt cgagggcacc gtgatcgcgg tcacccacga ccgctggttc
     2041 gcgcgcggat tcgaccgctt tgtggtcttc ggcgccgacg gttcggtgta cgaggcggac
     2101 gagccggtct gggacgaggg tcgggtggca aggagtcgct gaccgctgcc gtcgtccggc
     2161 gaatcgaaca ctgcgagatc ccggcttgag tccggatgac ggtttcgggg gttcgtcatt
     2221 ccgggcttgc tccgggatga cgggcaagcg gggtcgaaat cccgccttcc caccgcgctc
     2281 agccgtccaa ctccggcggc agcggttgct cgtggacgat tcgcaggccg ctgaccgccc
     2341 tggtcaggca cacgtacagc cggcgcagac ccgtcacccg gtcgggttcg ccggcgacga
     2401 tcgcagccgg ttcgaacagc accacctggt cgaactcgag gcccttggcc agggtggccg
     2461 gcaccacgtc caggcgatgg tcgaagtcct gcccggcggg ttcctccccc aggactccga
     2521 actcgagccc ggcatcgtcc agcgcgtcga tcgccgcggc cacggcgtcg tcggcgacga
     2581 tcaccccgac ggtgccctcc cgttcgagag tctcggtggc cgcccgggcc agctcggctg
     2641 tg
//
LOCUS       DAOSUG010000455         2640 bp    DNA     linear   ENV 10-JUN-2024
DEFINITION  MAG TPA_asm: Micropruina sp. isolate AMR_MDS_2195
            NODE_15688_length_2640_cov_4.169439, whole genome shotgun sequence.
ACCESSION   DAOSUG010000455 DAOSUG010000000
VERSION     DAOSUG010000455.1
DBLINK      BioProject: PRJNA925477
            BioSample: SAMN32864262
            Sequence Read Archive: SRR4236673
KEYWORDS    WGS; ENV; Metagenome Assembled Genome; MAG; Third Party Data; TPA;
            TPA:assembly.
SOURCE      Micropruina sp. (wastewater metagenome)
  ORGANISM  Micropruina sp.
            Bacteria; Bacillati; Actinomycetota; Actinomycetes;
            Propionibacteriales; Nocardioidaceae; Micropruina.
REFERENCE   1  (bases 1 to 2640)
  AUTHORS   Abdulkadir,N., Saraiva,J.P., Zhang,J., Stolte,S., Gillor,O.,
            Harms,H. and Rocha,U.
  TITLE     Genome-centric analyses of 165 metagenomes show that mobile genetic
            elements are crucial for the transmission of antimicrobial
            resistance genes to pathogens in activated sludge and wastewater
  JOURNAL   Microbiol Spectr 12 (3), e0291823 (2024)
   PUBMED   38289113
REFERENCE   2  (bases 1 to 2640)
  AUTHORS   Abdulkadir,N.
  TITLE     Direct Submission
  JOURNAL   Submitted (24-MAR-2023) Environmental Microbiology, Helmholtz
            Center for Environmental Research -UFZ, Permoserstr 15, Leipzig
            04318, Germany
COMMENT     The annotation was added by the NCBI Prokaryotic Genome Annotation
            Pipeline (PGAP). Information about PGAP can be found here:
            https://www.ncbi.nlm.nih.gov/genome/annotation_prok/
            
            ##Genome-Assembly-Data-START##
            Assembly Method        :: SPAdes v. 3.11.1
            Genome Representation  :: Full
            Expected Final Version :: Yes
            Genome Coverage        :: Not Applicable
            Sequencing Technology  :: Illumina MiSeq
            ##Genome-Assembly-Data-END##
            
            ##Genome-Annotation-Data-START##
            Annotation Provider               :: NCBI
            Annotation Date                   :: 05/04/2023 04:57:10
            Annotation Pipeline               :: NCBI Prokaryotic Genome
                                                 Annotation Pipeline (PGAP)
            Annotation Method                 :: Best-placed reference protein
                                                 set; GeneMarkS-2+
            Annotation Software revision      :: 6.5
            Features Annotated                :: Gene; CDS; rRNA; tRNA; ncRNA
            Genes (total)                     :: 2,776
            CDSs (total)                      :: 2,750
            Genes (coding)                    :: 2,714
            CDSs (with protein)               :: 2,714
            Genes (RNA)                       :: 26
            tRNAs                             :: 24
            ncRNAs                            :: 2
            Pseudo Genes (total)              :: 36
            CDSs (without protein)            :: 36
            Pseudo Genes (ambiguous residues) :: 0 of 36
            Pseudo Genes (frameshifted)       :: 2 of 36
            Pseudo Genes (incomplete)         :: 32 of 36
            Pseudo Genes (internal stop)      :: 2 of 36
            ##Genome-Annotation-Data-END##
FEATURES             Location/Qualifiers
     source          1..2640
                     /organism="Micropruina sp."
                     /mol_type="genomic DNA"
                     /submitter_seqid="NODE_15688_length_2640_cov_4.169439"
                     /isolate="AMR_MDS_2195"
                     /isolation_source="Activated sludge of municipal
                     wastewater treatment plants"
                     /db_xref="taxon:2737536"
                     /environmental_sample
                     /geo_loc_name="Canada"
                     /lat_lon="49.25 N 123.1 W"
                     /collection_date="2015-06-16"
                     /metagenome_source="wastewater metagenome"
                     /note="metagenomic"
     gene            complement(<1..958)
                     /locus_tag="PLF56_12645"
     CDS             complement(<1..958)
                     /locus_tag="PLF56_12645"
                     /inference="COORDINATES: protein motif:HMM:NF039608.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="hypothetical protein"
                     /protein_id="HOQ54447.1"
                     /translation="MDSGLGANVTCTIVNTDRPATLTLVKVVDNGTTGASAVPANWTL
                     SAIGPTTVTGPGNSASVTNQTVAAGSYSLAESGGPAGYTASSWSCTGGTLTGTSVAVA
                     NGGSVTCTITNTAVQPRLTLVKVVDNANTGATTVATAWTLTAAGPSTISGATGSGTVT
                     NATVPIGTFNLSETGPAGYTASSWVCTGGASATATSVTLGLGTSATCTITNTAIAPRL
                     TLVKRVVNQAGGTAVPTAWTLTGTGPVTITGASGSDAVTNAAVRVGGYALTETNGPPG
                     YSASTWSCDGGILTGANVTLALGTSATCTITNTDQPATLTLVK"
     gene            complement(1104..2474)
                     /locus_tag="PLF56_12650"
     CDS             complement(1104..2474)
                     /locus_tag="PLF56_12650"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_011074819.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_function: GO:0004803 - transposase activity [Evidence
                     IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="IS1380 family transposase"
                     /protein_id="HOQ54448.1"
                     /translation="MQLSHTPGRMFAAFDEPNLVGSAGLVPALALTGRIGLVELADAA
                     LTVPGSKGCAGAKLVSVLAGMLAGADSIDDLDVLRHGAMGKLFGGVRAPSTLGSFLRS
                     FRFGHVRQLDAVAARTLGRLAARVPGLLPVGPTVWLDVDDTIKPVYGRSKQGAEHGYT
                     HIRGLNAQIATVSGIEVAPVIVAARLRRGAASSAHGAVRLVRDALATLRRANVTDPVL
                     VRADSAYYQQPLVAAIITAGCQFSIGARLDKAVRARISTIEEQAWTPIVYPNAIVDPD
                     TAELISCAEVAEVDYTAFTSHGASKQVTARLIVRRVPERNTKKLAAAAQSGLFPLWRY
                     HAVFTNQTSPLVEAEKTHRGHAVIEQVFADLKAGPLAHLPSKSFNANAAWLAIATLAF
                     NLTRALGITAGDRYARAETATIRAQLIHTPARIATSARQILLHLPTRWPWATNWQQLW
                     TTIMRT"
BASE COUNT          378 a          852 c          951 g          459 t
ORIGIN      
        1 gcttgaccag ggtcagcgtc gccggctggt cggtgttggt gatggtgcag gtggcgctgg
       61 tgccgagcgc cagcgtgaca ttcgcgccgg tgaggatgcc accgtcgcag ctccaggtcg
      121 aggccgagta gccgggcggc ccgttggtct cggtcagcgc gtagccgccc acccgaaccg
      181 ccgcgttggt caccgcgtct gagccgctgg cgccggtgat ggtcaccgga cccgtgccgg
      241 tcaaggtcca ggctgtcggc accgccgtgc cgccggcctg gttgacgacc cgcttgacca
      301 aggtcagccg gggggcgatc gcggtgttgg tgatcgtgca ggtggccgaa gtgcccaggc
      361 ccagcgtgac gctggtcgcg gtcgccgacg cgcctcccgt acacacccac gacgaggccg
      421 tgtagccggc cgggccggtc tcggacaggt tgaacgtgcc gatgggcacc gtggcgttgg
      481 tgaccgttcc cgacccggtg gctccgctga tggtgctggg accggcagcg gtcagcgtcc
      541 aggcggtggc cacagtggtg gcaccggtgt ttgcgttgtc gaccaccttg accagggtca
      601 accgaggctg caccgccgta ttggtgatcg tgcaggtcac gctgccgccg ttcgcgaccg
      661 caacagaggt gccggtcagg gtgccgccgg tgcaactcca actcgaggcc gtgtagccgg
      721 cgggtccgcc cgactcggcc aggctgtacg agccggccgc aacggtctgg ttcgtcaccg
      781 aggccgagtt acccgggccg gtcacggtcg tcggacctat ggcgctcagc gtccagttgg
      841 ccgggacggc ggacgcaccg gtggtgccgt tgtccaccac cttgaccagg gtcaacgtcg
      901 ccggccggtc ggtgttgacg atggtgcagg tgacgtttgc gcctaaccct gaatccaccg
      961 gttgacgcgt gggggcgttt gtgcttgttg tgggtgggtt cggtggtggg tttgggggtc
     1021 ttggggcgtg gctggtcgcc cggtctgtcc gggttttcca ctggggggtt ccttggtcgt
     1081 ggtcggtcga ggggtgttca cggtcaggtg cgcatgattg tggtccacag ctgttgccaa
     1141 ttggtggccc agggccagcg ggtgggtagg tgcaggagaa tctggcgggc ggaggtggct
     1201 atccgtgccg gggtgtggat gagttgggcc cggatggtcg cggtttcagc gcgggcgtac
     1261 cggtcgccgg cggtgatgcc gagggcgcgg gtgaggttga acgcgagggt ggcgatggcg
     1321 agccaggcgg cgttggcgtt gaacgatttg gacggcaggt gggccagcgg gccggctttc
     1381 aggtcggcga acacctgctc gatcactgcg tgacctcggt gggtcttttc ggcctcgacc
     1441 agcgggctgg tctggttggt gaacaccgca tggtagcgcc acagagggaa caggccgctc
     1501 tgagcggcgg cggccagctt cttggtgttg cgttccggca cccggcgcac gatcaaccgg
     1561 gcggtgacct gcttgctggc tccatgcgag gtgaacgccg tgtagtcgac ctcggcgacc
     1621 tccgcgcagg agatcaactc tgcggtgtcg gggtccacga tcgcgttggg gtagacgatc
     1681 ggcgtccagg cctgctcctc gatggtgctg atcctggccc ggaccgcctt gtccaggcgg
     1741 gcaccgatcg agaactggca gcctgcggtg atgatcgccg caacgagcgg ctgctggtag
     1801 taggccgaat ccgcccggac cagcaccgga tcggtcacgt tcgcccgtcg cagcgtggcg
     1861 agcgcatcac gcaccagccg gaccgcgccg tgcgcggaac tggctgcgcc acgacgcagc
     1921 cgggccgcca cgatcaccgg cgcgacctcg atgccggaca cggtggcgat ctgggcgttc
     1981 agcccgcgga tgtgggtata gccgtgctca gcaccctgct tgctgcggcc gtacaccggt
     2041 ttgatcgtgt catccacatc cagccacacc gtcgggccga caggtagtag cccgggcacc
     2101 cgtgcggcca gtcggcccag ggtccgcgcg gcgaccgcat cgagttggcg aacatgcccg
     2161 aaccggaacg accgcaggaa gctgcccagc gtcgagggtg cccgcacccc gccgaacagt
     2221 ttccccatcg ccccatgccg taacacgtcc aggtcgtcga tgctgtccgc gccggccagc
     2281 atcccggcca gcacagacac cagcttcgcc ccggcacagc ccttcgaccc gggcaccgtc
     2341 aacgccgcat ccgccaactc caccagcccg atccggcccg tgagcgccaa cgcaggcacc
     2401 aaccccgctg aacccaccag gttcggctcg tcgaacgcgg cgaacatgcg gccgggggta
     2461 tgagacagtt gcatctcgga agtgcccttc ctgcagggag taacggaacc tagacaagtc
     2521 ccattctccc ttgccagaag ggcatttcct cattcccacg ccaccaccag accggtggat
     2581 tcaggattag tggcccgtcg ccccgtggcg ctcgtgttcg cgttgactca tcgaagatgc
//
LOCUS       DAOSUG010000456         2637 bp    DNA     linear   ENV 10-JUN-2024
DEFINITION  MAG TPA_asm: Micropruina sp. isolate AMR_MDS_2195
            NODE_15713_length_2637_cov_5.203718, whole genome shotgun sequence.
ACCESSION   DAOSUG010000456 DAOSUG010000000
VERSION     DAOSUG010000456.1
DBLINK      BioProject: PRJNA925477
            BioSample: SAMN32864262
            Sequence Read Archive: SRR4236673
KEYWORDS    WGS; ENV; Metagenome Assembled Genome; MAG; Third Party Data; TPA;
            TPA:assembly.
SOURCE      Micropruina sp. (wastewater metagenome)
  ORGANISM  Micropruina sp.
            Bacteria; Bacillati; Actinomycetota; Actinomycetes;
            Propionibacteriales; Nocardioidaceae; Micropruina.
REFERENCE   1  (bases 1 to 2637)
  AUTHORS   Abdulkadir,N., Saraiva,J.P., Zhang,J., Stolte,S., Gillor,O.,
            Harms,H. and Rocha,U.
  TITLE     Genome-centric analyses of 165 metagenomes show that mobile genetic
            elements are crucial for the transmission of antimicrobial
            resistance genes to pathogens in activated sludge and wastewater
  JOURNAL   Microbiol Spectr 12 (3), e0291823 (2024)
   PUBMED   38289113
REFERENCE   2  (bases 1 to 2637)
  AUTHORS   Abdulkadir,N.
  TITLE     Direct Submission
  JOURNAL   Submitted (24-MAR-2023) Environmental Microbiology, Helmholtz
            Center for Environmental Research -UFZ, Permoserstr 15, Leipzig
            04318, Germany
COMMENT     The annotation was added by the NCBI Prokaryotic Genome Annotation
            Pipeline (PGAP). Information about PGAP can be found here:
            https://www.ncbi.nlm.nih.gov/genome/annotation_prok/
            
            ##Genome-Assembly-Data-START##
            Assembly Method        :: SPAdes v. 3.11.1
            Genome Representation  :: Full
            Expected Final Version :: Yes
            Genome Coverage        :: Not Applicable
            Sequencing Technology  :: Illumina MiSeq
            ##Genome-Assembly-Data-END##
            
            ##Genome-Annotation-Data-START##
            Annotation Provider               :: NCBI
            Annotation Date                   :: 05/04/2023 04:57:10
            Annotation Pipeline               :: NCBI Prokaryotic Genome
                                                 Annotation Pipeline (PGAP)
            Annotation Method                 :: Best-placed reference protein
                                                 set; GeneMarkS-2+
            Annotation Software revision      :: 6.5
            Features Annotated                :: Gene; CDS; rRNA; tRNA; ncRNA
            Genes (total)                     :: 2,776
            CDSs (total)                      :: 2,750
            Genes (coding)                    :: 2,714
            CDSs (with protein)               :: 2,714
            Genes (RNA)                       :: 26
            tRNAs                             :: 24
            ncRNAs                            :: 2
            Pseudo Genes (total)              :: 36
            CDSs (without protein)            :: 36
            Pseudo Genes (ambiguous residues) :: 0 of 36
            Pseudo Genes (frameshifted)       :: 2 of 36
            Pseudo Genes (incomplete)         :: 32 of 36
            Pseudo Genes (internal stop)      :: 2 of 36
            ##Genome-Annotation-Data-END##
FEATURES             Location/Qualifiers
     source          1..2637
                     /organism="Micropruina sp."
                     /mol_type="genomic DNA"
                     /submitter_seqid="NODE_15713_length_2637_cov_5.203718"
                     /isolate="AMR_MDS_2195"
                     /isolation_source="Activated sludge of municipal
                     wastewater treatment plants"
                     /db_xref="taxon:2737536"
                     /environmental_sample
                     /geo_loc_name="Canada"
                     /lat_lon="49.25 N 123.1 W"
                     /collection_date="2015-06-16"
                     /metagenome_source="wastewater metagenome"
                     /note="metagenomic"
     gene            complement(<1..204)
                     /locus_tag="PLF56_12655"
     CDS             complement(<1..204)
                     /locus_tag="PLF56_12655"
                     /inference="COORDINATES: ab initio
                     prediction:GeneMarkS-2+"
                     /note="Derived by automated computational analysis using
                     gene prediction method: GeneMarkS-2+."
                     /codon_start=1
                     /transl_table=11
                     /product="hypothetical protein"
                     /protein_id="HOQ54449.1"
                     /translation="MGAEEFGSFRLLGWERAASTYEGVPFTGPGGAVISVERYLAKVL
                     ALVQGEVPPVESVVLSAALGRVLA"
     gene            290..2455
                     /locus_tag="PLF56_12660"
     CDS             290..2455
                     /locus_tag="PLF56_12660"
                     /inference="COORDINATES: protein motif:HMM:NF015338.2"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_function: GO:0005515 - protein binding [Evidence IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="fibronectin type III domain-containing protein"
                     /protein_id="HOQ54450.1"
                     /translation="MKSLRAAVLLCVTCLVVLGVAPPPADAAAALATPAKPATLSTST
                     EAISLSWKSVSGAAGYRVQYAISSAFTAPKSVKASRNAVDITALKSGTTYYLRVAALN
                     SSGDVVSKNSSALKASTRSSGYSKLAPRDLSVTKKSATSVSLTWSSRGAGIRYRVAYA
                     TSASFKKVVYQRETSTGLTLSNLRPNTSYHLKVRVITSSGANLSSYSPAITATTSPLA
                     APKGLRVTASAKKALALNWTSVSGAERYRVQYAKSASMSGAKYVRFTGAAGELSGLAS
                     GATYYLKVRAITPSGANLSNYSAAIKATTATSSSATYLPPTGLNGTSTAPGKLTVRWT
                     SRGAGLAYQVQYSTSSAMSSAASAKTSGGSLTLSGLGNAIPYHLRVRVVSGAGTPLSA
                     FSPTVTLTTRTDQAVDLRVASYNIKCTNCYSGLPNEGTWYQRRDSVVATVKGQRPDVI
                     GFQEASQGWLKDGAGNPVSKSQFEDLLERLGAPFKITNSYRNNCVKSTTPTNCVYQDR
                     GASQGTRIVYNSSTLALIDQGSKKLSKVVTSDPDRYAAWAILEQKQTGRQFLFVDTHL
                     EFRSDAAGSRSYYNLRIAQTREALALIAAKRKNLPVYFVGDFNSSKWASPSNGPYDVM
                     RAAGFVDPLGNSYQSTSKTSGAIVGRRIRTNFSSYNNFETKARSFSYVNGTYIDYIWV
                     SKGIEVPEWETVVNVDAAGNFVGRIPSDHNMLRATTRLP"
     gene            complement(2447..>2637)
                     /locus_tag="PLF56_12665"
     CDS             complement(2447..>2637)
                     /locus_tag="PLF56_12665"
                     /inference="COORDINATES: ab initio
                     prediction:GeneMarkS-2+"
                     /note="Derived by automated computational analysis using
                     gene prediction method: GeneMarkS-2+."
                     /codon_start=3
                     /transl_table=11
                     /product="hypothetical protein"
                     /protein_id="HOQ54451.1"
                     /translation="FVAARPAQGLQVRFSNPADQSAFAASAAQGLSSERIAALGWRPR
                     VSLAEGIDRTLRHHEASR"
BASE COUNT          498 a          936 c          790 g          413 t
ORIGIN      
        1 ggccagcacc cgtcccaggg ctgcggaaag cacgacgctt tcgacagggg gcacctcgcc
       61 ctgcaccagc gccaacacct tcgccaggta ccgctcgacg ctgatcacgg cgccgcccgg
      121 cccggtgaac gggacacctt cgtacgttga tgctgcacgc tcccaaccta atagccgaaa
      181 ggaaccgaac tcttcagcgc ccattgaggg tcaggtacgg cgcgttggct tgacgtgagc
      241 tgagtgatta ctctcaaaac tcaccctcct ctcatgttcg gagccgtccg tgaagagttt
      301 gcgagccgct gtgctgctct gcgtcacctg cctggtcgtg ctcggcgtcg caccgccccc
      361 agccgacgcg gccgcagcgt tggccacccc ggccaagccc gccacgctca gcacgagtac
      421 cgaggcgatt tcactgtcat ggaaatccgt cagcggagca gccggttacc gggtgcaata
      481 cgcgatctcg tccgcgttca cggcgccgaa gtcggtgaag gcgagccgca atgcggtcga
      541 catcaccgcg ctgaaatccg gcaccacgta ctacctgcgg gtcgcggcac tgaattcgtc
      601 cggcgatgtg gtgagcaaga actcgtccgc actgaaggcg tccacccgat cctcgggcta
      661 ctcgaaactg gcgccccgcg acctgtcggt caccaagaag tcggccacct cggtgagcct
      721 cacctggagc agccgcggcg ccggcattcg ctaccgggtg gcatacgcca cgtcggcgtc
      781 gttcaagaaa gtggtgtacc agcgcgagac ctcgaccggg ctcacgctca gcaatctgag
      841 acccaacacc tcctatcacc tcaaggtgcg ggtgatcacc tcgtccgggg ccaacctgag
      901 ttcgtactcg ccggcgatca ccgcgaccac ctcaccgctc gcggccccca agggcctgcg
      961 ggtcaccgcg agcgcaaaga aggctctcgc gctcaactgg acgtccgtca gcggcgccga
     1021 gcgctaccgt gtccaatacg cgaagtcggc gtcgatgtcg ggtgcgaaat acgtccgctt
     1081 caccggtgcc gccggcgagt tgagcggctt ggcgagcggc gccacctact acctgaaggt
     1141 gcgcgccatc acaccttctg gcgccaacct gagcaactac tcagcggcga tcaaggcaac
     1201 cacggccacc tcgagcagcg ccacgtatct gcccccgacg ggcctcaacg gcacctcgac
     1261 ggcaccgggc aagctgaccg ttcgatggac ctcgcgcggc gccggcctcg cctatcaggt
     1321 gcaatactcg accagttcgg cgatgagcag cgccgcctcg gcgaaaacgt caggcggttc
     1381 gttgaccctc tccgggctcg gcaacgcgat cccataccac ctcagggtgc gggtggtgag
     1441 cggcgccggt acgccactga gcgctttctc gccgacggtg acgctcacca cccgcaccga
     1501 ccaggcggtc gacctgcgcg tcgcgtcgta caacatcaaa tgcaccaact gctacagcgg
     1561 cctgcccaac gagggcacct ggtaccagcg cagggacagc gtggtggcca ccgtcaaggg
     1621 ccagcggccg gacgtgatcg gatttcagga ggcctcgcag ggctggctca aggacggcgc
     1681 cggcaacccg gtcagcaaat cccagttcga agacctgctc gaacggctcg gcgcgccctt
     1741 caaaatcacc aacagctatc gcaacaactg tgtgaagtcg acgacgccca ccaactgcgt
     1801 gtaccaggac cgtggcgctt cacagggcac ccggatcgtc tacaactcca gcacccttgc
     1861 cctgatcgac cagggctcga agaagctgtc gaaggtcgtc acgtccgacc cggaccgcta
     1921 cgcggcctgg gcgatcctgg aacagaagca gaccggccgc cagttcctgt tcgtcgatac
     1981 ccatcttgag tttcgctcgg acgcggcggg ctcgcgcagc tactacaacc tgcgcatcgc
     2041 gcagacccgc gaggcgctcg ccctgatcgc agccaagcgc aagaacctgc cggtgtactt
     2101 cgtcggcgac ttcaactcca gcaagtgggc gtcgccgtcc aacggcccct atgacgtgat
     2161 gagggcggcc ggctttgtcg atccgctggg caacagctac cagtcgacca gcaagaccag
     2221 cggcgccatc gtcggcaggc gcatccgcac caacttctcg agctacaaca acttcgagac
     2281 caaggcgcgt tcgttcagtt acgtcaacgg cacctacatc gactacatct gggtgtcgaa
     2341 gggtatcgag gtgcccgagt gggagacggt ggtcaacgtg gacgccgccg gcaacttcgt
     2401 tggtcgcatt ccgtccgatc acaacatgct gcgggcgaca acgcggctac cgtgacgcct
     2461 cgtggtgccg cagggtgcga tcgatgccct cggccagcga cactcgtggc cgccagccga
     2521 gcgccgcgat gcgttcactg ctcaacccct gcgccgcgga cgcggcgaac gccgactgat
     2581 cggccgggtt gctgaaccgc acctgcaacc cctgcgccgg ccgtgctgcc acgaaca
//
LOCUS       DAOSUG010000457         2629 bp    DNA     linear   ENV 10-JUN-2024
DEFINITION  MAG TPA_asm: Micropruina sp. isolate AMR_MDS_2195
            NODE_15781_length_2629_cov_5.089744, whole genome shotgun sequence.
ACCESSION   DAOSUG010000457 DAOSUG010000000
VERSION     DAOSUG010000457.1
DBLINK      BioProject: PRJNA925477
            BioSample: SAMN32864262
            Sequence Read Archive: SRR4236673
KEYWORDS    WGS; ENV; Metagenome Assembled Genome; MAG; Third Party Data; TPA;
            TPA:assembly.
SOURCE      Micropruina sp. (wastewater metagenome)
  ORGANISM  Micropruina sp.
            Bacteria; Bacillati; Actinomycetota; Actinomycetes;
            Propionibacteriales; Nocardioidaceae; Micropruina.
REFERENCE   1  (bases 1 to 2629)
  AUTHORS   Abdulkadir,N., Saraiva,J.P., Zhang,J., Stolte,S., Gillor,O.,
            Harms,H. and Rocha,U.
  TITLE     Genome-centric analyses of 165 metagenomes show that mobile genetic
            elements are crucial for the transmission of antimicrobial
            resistance genes to pathogens in activated sludge and wastewater
  JOURNAL   Microbiol Spectr 12 (3), e0291823 (2024)
   PUBMED   38289113
REFERENCE   2  (bases 1 to 2629)
  AUTHORS   Abdulkadir,N.
  TITLE     Direct Submission
  JOURNAL   Submitted (24-MAR-2023) Environmental Microbiology, Helmholtz
            Center for Environmental Research -UFZ, Permoserstr 15, Leipzig
            04318, Germany
COMMENT     The annotation was added by the NCBI Prokaryotic Genome Annotation
            Pipeline (PGAP). Information about PGAP can be found here:
            https://www.ncbi.nlm.nih.gov/genome/annotation_prok/
            
            ##Genome-Assembly-Data-START##
            Assembly Method        :: SPAdes v. 3.11.1
            Genome Representation  :: Full
            Expected Final Version :: Yes
            Genome Coverage        :: Not Applicable
            Sequencing Technology  :: Illumina MiSeq
            ##Genome-Assembly-Data-END##
            
            ##Genome-Annotation-Data-START##
            Annotation Provider               :: NCBI
            Annotation Date                   :: 05/04/2023 04:57:10
            Annotation Pipeline               :: NCBI Prokaryotic Genome
                                                 Annotation Pipeline (PGAP)
            Annotation Method                 :: Best-placed reference protein
                                                 set; GeneMarkS-2+
            Annotation Software revision      :: 6.5
            Features Annotated                :: Gene; CDS; rRNA; tRNA; ncRNA
            Genes (total)                     :: 2,776
            CDSs (total)                      :: 2,750
            Genes (coding)                    :: 2,714
            CDSs (with protein)               :: 2,714
            Genes (RNA)                       :: 26
            tRNAs                             :: 24
            ncRNAs                            :: 2
            Pseudo Genes (total)              :: 36
            CDSs (without protein)            :: 36
            Pseudo Genes (ambiguous residues) :: 0 of 36
            Pseudo Genes (frameshifted)       :: 2 of 36
            Pseudo Genes (incomplete)         :: 32 of 36
            Pseudo Genes (internal stop)      :: 2 of 36
            ##Genome-Annotation-Data-END##
FEATURES             Location/Qualifiers
     source          1..2629
                     /organism="Micropruina sp."
                     /mol_type="genomic DNA"
                     /submitter_seqid="NODE_15781_length_2629_cov_5.089744"
                     /isolate="AMR_MDS_2195"
                     /isolation_source="Activated sludge of municipal
                     wastewater treatment plants"
                     /db_xref="taxon:2737536"
                     /environmental_sample
                     /geo_loc_name="Canada"
                     /lat_lon="49.25 N 123.1 W"
                     /collection_date="2015-06-16"
                     /metagenome_source="wastewater metagenome"
                     /note="metagenomic"
     gene            <1..332
                     /locus_tag="PLF56_12670"
     CDS             <1..332
                     /locus_tag="PLF56_12670"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_013161395.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=3
                     /transl_table=11
                     /product="TerC family protein"
                     /protein_id="HOQ54452.1"
                     /translation="LTQEPFLVFTANVFALMGLRQLYFLIGGLLQRLVYLSVGLSFIL
                     AFIGVKLVLHALHEYHLDDAWGINVDIPIWLSLLVIIGTLTVTTVASLIKSKRDADRE
                     RALDGPA"
     gene            complement(948..1562)
                     /locus_tag="PLF56_12675"
     CDS             complement(948..1562)
                     /locus_tag="PLF56_12675"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_002518341.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_function: GO:0016787 - hydrolase activity [Evidence
                     IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="MBL fold metallo-hydrolase"
                     /protein_id="HOQ54453.1"
                     /translation="MLIGEGVRLAKTSVGPMDNNAYLLTHDNDALLLDAAAEPEVLLR
                     QLDCLDLVGVVTTHHHHDHIGALAEVVEGTGAPAFAGAPDAAAITEATGVTCREVWTG
                     DAIELGPVLLEVIGLVGHTPGSIALALTPSDGPVRLFTGDSLFPGGVGKTWKPGDFDT
                     LLADVTAKLFDRFGDDTIVHPGHGDDTTLGAERPHLGEWRARGW"
     gene            complement(1731..2444)
                     /locus_tag="PLF56_12680"
     CDS             complement(1731..2444)
                     /locus_tag="PLF56_12680"
                     /inference="COORDINATES: protein motif:HMM:TIGR03083.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_function: GO:0046872 - metal ion binding [Evidence
                     IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="maleylpyruvate isomerase family
                     mycothiol-dependent enzyme"
                     /protein_id="HOQ54454.1"
                     /translation="MTAQTFSAPLETLRKRVQEATQRLLGDTIGISDDDWNGPSGLPG
                     WSRAHVAAHLAANADALAGVLRAAMDHRESALYPDAAARLAGIERGSSLTGLELQISL
                     DTSAGRLERVLDDIDDPTLPVHLPGRSLTVTQVAMARLSEVIVHHLDLNCDFGVDRVE
                     QSTGRWLLQWALLWHAEDFSLPSVRVESTSGLSAELGAGEPVRTVTGDDAALWAWLVG
                     RSAEPNPEGEDAARPIRVS"
BASE COUNT          400 a          907 c          862 g          460 t
ORIGIN      
        1 gcctgaccca ggagccgttc ctggtgttca ccgccaacgt gttcgcgctg atggggctgc
       61 gccagctgta cttcctcatc ggtggcctac tgcagcggct ggtctacctg tcggtcggcc
      121 tgtcgttcat cctggccttc atcggcgtca agttggttct gcacgcactg cacgagtacc
      181 acctggacga tgcgtggggc atcaacgtcg acattccgat ctggctctcg ttgctggtga
      241 tcatcggcac cctgacggtc accacggtgg ccagcctgat caaatccaaa cgcgacgccg
      301 accgcgagcg ggcgctggac ggtcccgcct agcacactga gcgcaagggc gactcggtgg
      361 tcgaactgtc cagggacgtg ccggtcaatg ctccatccct tggccgagct gtctaaaccc
      421 ctgctcacga gggggtcccg accagcggcg tacaaggctc tcgaccagcg gcgcacaagg
      481 ctctcgacca gcgggcgccc tcgtcatccc gggcttgtcc cgggatctcg gcgcacctca
      541 gagatccctg cgtgcgccgg gatgacgtgg ctttttggca ccgagttctg gggcgctctg
      601 cgggatcctg cgtgcgccgg gacgacgtgg ctttttcggc accgagttct ggggcgctct
      661 gccagatcct gcggacgccg ggatgacgtc acttcttcgg caccgagttc tggggcgttt
      721 gcgagatccc gcgtgctccc gggatgacgg cggtggcgac gttcagggaa gcgctgatca
      781 atgcgcgacg cgttggtgga gcatggcggg aaaatcgcgc ggaccccgac aggcccaacc
      841 agcgatagat ccgtgcctgc acctttctcg cgggaatctc gacaggctcg tccagggatg
      901 gatcagcgcc tctccatcac tcgttgggcg ccgtccagcg gcgggactca ccagcctcgg
      961 gcacgccatt cgcccaggtg tggacgctcc gcgcccagcg tggtgtcgtc gccgtgacca
     1021 ggatgcacga tcgtgtcgtc gccgaaccgg tcgaacagct tggcggtcac gtcggcgagc
     1081 aaagtgtcga agtctcccgg cttccaggtc ttgcccacgc cgcccggaaa caggctgtca
     1141 ccggtgaaca ggcgcaccgg gccgtccgac ggcgtcagcg ccagcgcgat cgaaccgggg
     1201 gtgtggccga ccaggccgat cacctcaagc agcaccggac ccagttcgat cgcgtcgccc
     1261 gtccacactt cgcgacaggt gacgccggtg gcctcggtga tcgctgccgc gtccggtgcg
     1321 cccgcgaatg cgggtgcgcc ggtgccttcg acgacctcgg cgagagcgcc gatgtggtcg
     1381 tggtggtggt gggtggtgac gaccccgacc agatcgaggc agtccagttg ccgaagcagt
     1441 acctcaggct cggcggccgc gtcgagcagc agggcgtcgt tgtcgtgggt cagcaggtag
     1501 gcgttgttgt ccatcggtcc gacggacgtc ttggccagcc gtacaccctc tccgatcagc
     1561 atggttcatg attgcagtcc cagggcgtgt gtcctaaccc tggccccgct gcggcgcagc
     1621 cagtacgcct gctcgctacg ttgtcactcg cggcgagagg acccactatc gactgtcttc
     1681 cgccctgcgc ccagacgcac actgggcacg tcctctcgga acgcccaggg ttaggagaca
     1741 cggattggcc gggctgcgtc ctcaccctcg gggttcggtt ccgcggatcg gcccaccagc
     1801 caggcccaca gggcggcgtc gtcgccggtg accgtgcgca ccggttcgcc cgcgcccaac
     1861 tcggcgctga gcccggacgt gctctccacc cgcacgctcg gcagcgagaa atcctccgcg
     1921 tgccacagca gagcccactg cagcaaccac cgtccggtgc tctgctcgac gcggtcgacg
     1981 ccgaagtcgc agttcagatc cagatggtgc acgatcacct cggacaggcg tgccatggcc
     2041 acctgggtca cggtgaggct gcggcccggc aggtgaaccg gcagggtcgg gtcgtcgatg
     2101 tcgtccagca cgcgctccag ccgcccggcc gaggtgtcga ggctgatctg caactccagt
     2161 ccggtcaggc tcgatcccct ttcgatgccg gccagacgtg ccgccgcgtc agggtacagc
     2221 gcgctctcgc ggtggtccat cgcagccctc agcaccccgg cgagggcatc ggcgttggcc
     2281 gccagatgcg cggccacatg ggccctcgac cagccgggga ggccgctcgg accgttccag
     2341 tcgtcgtccg aaatgccgat ggtgtcgccg agcagccgct gcgtcgcctc ctggacgcgc
     2401 ttgcgcaatg tctccagcgg cgctgaaaac gtctgtgcgg tcatgaccgc caccctaagg
     2461 ggtcgctcgc accctgccca cctgcccggc gcaagaactg tcagtggcag cgcatagcat
     2521 gatcgccgtg aatgaacggc tgatcgtccg aggtgctcgc gagcacaacc tgcgcaatgt
     2581 gaacctcgac ctgccgcgcg atgccctgat cgtttttacc ggcttgtcg
//
LOCUS       DAOSUG010000458         2604 bp    DNA     linear   ENV 10-JUN-2024
DEFINITION  MAG TPA_asm: Micropruina sp. isolate AMR_MDS_2195
            NODE_16002_length_2604_cov_3.395449, whole genome shotgun sequence.
ACCESSION   DAOSUG010000458 DAOSUG010000000
VERSION     DAOSUG010000458.1
DBLINK      BioProject: PRJNA925477
            BioSample: SAMN32864262
            Sequence Read Archive: SRR4236673
KEYWORDS    WGS; ENV; Metagenome Assembled Genome; MAG; Third Party Data; TPA;
            TPA:assembly.
SOURCE      Micropruina sp. (wastewater metagenome)
  ORGANISM  Micropruina sp.
            Bacteria; Bacillati; Actinomycetota; Actinomycetes;
            Propionibacteriales; Nocardioidaceae; Micropruina.
REFERENCE   1  (bases 1 to 2604)
  AUTHORS   Abdulkadir,N., Saraiva,J.P., Zhang,J., Stolte,S., Gillor,O.,
            Harms,H. and Rocha,U.
  TITLE     Genome-centric analyses of 165 metagenomes show that mobile genetic
            elements are crucial for the transmission of antimicrobial
            resistance genes to pathogens in activated sludge and wastewater
  JOURNAL   Microbiol Spectr 12 (3), e0291823 (2024)
   PUBMED   38289113
REFERENCE   2  (bases 1 to 2604)
  AUTHORS   Abdulkadir,N.
  TITLE     Direct Submission
  JOURNAL   Submitted (24-MAR-2023) Environmental Microbiology, Helmholtz
            Center for Environmental Research -UFZ, Permoserstr 15, Leipzig
            04318, Germany
COMMENT     The annotation was added by the NCBI Prokaryotic Genome Annotation
            Pipeline (PGAP). Information about PGAP can be found here:
            https://www.ncbi.nlm.nih.gov/genome/annotation_prok/
            
            ##Genome-Assembly-Data-START##
            Assembly Method        :: SPAdes v. 3.11.1
            Genome Representation  :: Full
            Expected Final Version :: Yes
            Genome Coverage        :: Not Applicable
            Sequencing Technology  :: Illumina MiSeq
            ##Genome-Assembly-Data-END##
            
            ##Genome-Annotation-Data-START##
            Annotation Provider               :: NCBI
            Annotation Date                   :: 05/04/2023 04:57:10
            Annotation Pipeline               :: NCBI Prokaryotic Genome
                                                 Annotation Pipeline (PGAP)
            Annotation Method                 :: Best-placed reference protein
                                                 set; GeneMarkS-2+
            Annotation Software revision      :: 6.5
            Features Annotated                :: Gene; CDS; rRNA; tRNA; ncRNA
            Genes (total)                     :: 2,776
            CDSs (total)                      :: 2,750
            Genes (coding)                    :: 2,714
            CDSs (with protein)               :: 2,714
            Genes (RNA)                       :: 26
            tRNAs                             :: 24
            ncRNAs                            :: 2
            Pseudo Genes (total)              :: 36
            CDSs (without protein)            :: 36
            Pseudo Genes (ambiguous residues) :: 0 of 36
            Pseudo Genes (frameshifted)       :: 2 of 36
            Pseudo Genes (incomplete)         :: 32 of 36
            Pseudo Genes (internal stop)      :: 2 of 36
            ##Genome-Annotation-Data-END##
FEATURES             Location/Qualifiers
     source          1..2604
                     /organism="Micropruina sp."
                     /mol_type="genomic DNA"
                     /submitter_seqid="NODE_16002_length_2604_cov_3.395449"
                     /isolate="AMR_MDS_2195"
                     /isolation_source="Activated sludge of municipal
                     wastewater treatment plants"
                     /db_xref="taxon:2737536"
                     /environmental_sample
                     /geo_loc_name="Canada"
                     /lat_lon="49.25 N 123.1 W"
                     /collection_date="2015-06-16"
                     /metagenome_source="wastewater metagenome"
                     /note="metagenomic"
     gene            complement(<1..892)
                     /locus_tag="PLF56_12685"
     CDS             complement(<1..892)
                     /locus_tag="PLF56_12685"
                     /inference="COORDINATES: protein motif:HMM:NF025080.2"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="transposase"
                     /protein_id="HOQ54455.1"
                     /translation="MRVCHTLDPVFDDPNLIAFGGLPAVLTLAEQAGLHDLVDEHVTV
                     PGTAGANASVKVTAIIAGMIAGADSISDLDVLRHGGTGRVFTGLRAATTLGTHLRGYR
                     FGHVRQLDAVAARLLGNLARVSPILTGADQLVYVDVDDTVRETHGYRKQGAAYGYSRV
                     KGLNAQVAIVSTPLAAPVIAATRLRKGNIDSGHGAARLIGDSLATLRRATRPGLVVVR
                     ADSAYFRHDVVAAATAGGARFSVTARMNKAVVRAIAGIDEQAWTTIRYRDAIYDEVEQ
                     RWISEAEVAEVPFTAFTSHRQ"
     gene            complement(1054..2283)
                     /locus_tag="PLF56_12690"
     CDS             complement(1054..2283)
                     /locus_tag="PLF56_12690"
                     /inference="COORDINATES: ab initio
                     prediction:GeneMarkS-2+"
                     /note="Derived by automated computational analysis using
                     gene prediction method: GeneMarkS-2+."
                     /codon_start=1
                     /transl_table=11
                     /product="hypothetical protein"
                     /protein_id="HOQ54456.1"
                     /translation="MSTRARGVGVSVLVLAGASALVSACSSLPTWQDVSESAATLGST
                     RVALYVSPQSYLWGGPGHVILGQDATTPLKALKTSDMDNARLTWTHRGLFFSDTRRDY
                     LLGDRLATTDSPKTNSQVVAFSSPDGSSTWSLLIEGLTGDTYTEQAITFNGEVSVRYD
                     VDGFNQPIARCRDGIFGVAILAGPSNVKKAAGRGVVSSNGSDMPQMLTRLHPPAFEPG
                     GAFVAAVPAYMVDYFWNDAACDADGNAIFLAKDNHVDAPKTFEIRTWNVRTGKLTRHA
                     LRLPDASEPSMDTSRPVFFGRADRVLHDGKLMWIDSDGGVHLTDPATGATTLAFTTGV
                     ALRESDEYSLMRFERTWLLIRQIDENPDLPMILHRFDTSTGAGQIIAELPSIQRELSA
                     ALVLRDFAVAPELLAAP"
BASE COUNT          476 a          830 c          838 g          460 t
ORIGIN      
        1 cctggcggtg ggacgtgaac gcggtgaacg gcacctcggc gacctcagcc tccgagatcc
       61 agcgttgctc gacctcgtcg tagatcgcgt cgcggtagcg gatcgtggtc cacgcctgct
      121 cgtcgatgcc ggcgatggcc cggaccacgg ccttgttcat ccgggcggtg accgagaacc
      181 gggccccgcc cgcggtcgcg gcggcgacca cgtcgtgacg gaagtaggca ctgtcagcgc
      241 gcacgaccac cagacccggg cgggtggccc gccgcagggt ggcgaggctg tcgccgatca
      301 ggcgggcggc accgtgtccg gagtcgatgt tgcccttccg caacctggtg gcggcgatca
      361 ccggcgcagc caacggggtc gacacgatcg ccacctgggc gttcagcccc ttcacccggc
      421 tgtagccgta ggcggcaccc tgcttgcggt agccgtgggt ttcccgcacc gtgtcgtcca
      481 cgtcgacgta cacgagttgg tcggcgccgg tgaggatcgg cgacaccctc gccaggttgc
      541 ccagcaggcg ggcggcgacc gcgtccaact gccggacgtg cccgaaccgg tagccacgca
      601 ggtgggtgcc caacgtggtc gctgcccgca acccggtgaa cacgcgcccc gtcccgccat
      661 ggcgcagcac gtccagatcc gaaatcgagt ccgcgccggc gatcatgccc gcgatgatcg
      721 ccgtcacctt gaccgacgcg ttcgccccgg ccgtgcccgg caccgtcaca tgctcatcga
      781 ccaggtcgtg caggccagcc tgttcggcca acgtcagcac cgccggcagg ccaccgaagg
      841 cgatcaggtt cgggtcgtcg aaaaccgggt caagggtatg gcagactcgc actacgagtg
      901 ctcctgtcaa ggatcagaac ggcgtcttcg caaacaccat tcttccttgt cacaggagca
      961 ttcgccatca cggcacaccc atcaccccgg cggatcgagg ctcagctcag ctcacccagc
     1021 tcagctcact ggtccgttca gttcagattc agttcacggt gcggcgagaa gttcgggcgc
     1081 gactgcgaag tcacggagga cgagggcagc cgacaactcg cgctggatcg agggcagttc
     1141 ggcgatgatc tgcccggccc cggtggaggt gtcgaaccga tgaagaatca tgggcaggtc
     1201 aggattctcg tcgatctgcc ggatcaacag ccacgtgcgt tcgaatcgca tcagcgagta
     1261 ctcgtccgac tcgcggagcg ccacgccggt tgtgaaggcg agagtggttg ccccagtggc
     1321 gggatcagtg aggtgaacgc cgccgtcgga gtcgatccac atgagtttgc cgtcatgcaa
     1381 caccctgtct gcacgcccga agaacactgg cctcgaggta tccatggacg gttcacttgc
     1441 gtcgggaagg cggagggcgt gtcgggtgag ctttcctgta cgtacgttcc acgtgcggat
     1501 ctcgaacgtt ttcggagcat ccacgtgatt gtccttcgcc aggaagatgg cgttgccatc
     1561 agcgtcacat gccgcatcgt tccagaaata gtcgaccatg taggcgggga cggccgccac
     1621 gaaggcaccg ccgggctcga aagcgggagg atgaaggcgg gtgagcatct gaggcatatc
     1681 cgaaccgttc gagctgacaa ctcctcgccc cgcggccttc ttgacgttgc tcgggcccgc
     1741 caggatggcg accccgaaga ttccgtcccg gcaccgtgcg atcggctggt tgaagccatc
     1801 gacgtcgtaa cgaacgctca cctctccgtt gaacgtgatg gcctgctcgg tgtaggtgtc
     1861 accggtcagc ccctcgatga gaagcgacca tgtactgctg ccgtccggag acgaaaatgc
     1921 gaccacctgg ctgtttgtct tggggctgtc ggtggtggcg aggcggtcac ccaggaggta
     1981 gtcgcgccgg gtgtcggaga agaacagacc ccggtgcgtc caggtcagac gagcgttgtc
     2041 catatcgctg gtcttcagcg ctttgagcgg tgtggtggcg tcctgtccca ggatgacgtg
     2101 gcccggcccg ccccacagat agctctgcgg cgagacgtac agtgccacgc gcgtggaccc
     2161 gagcgtggcg gccgactctg acacgtcttg ccacgtgggc aacgacgaac acgcggaaac
     2221 caacgcgctc gcgcccgcca gaaccaaaac gctcacgccc acgccacgtg cgcgtgtact
     2281 cacgtgacga tcctgtaggt cgacgtgagc gcagaattgg tcagatcagt gggatacacg
     2341 cccaaccctc cgttggcatc cagatcaata ccagttgctt ctctgtaggc caaccacacg
     2401 agttgcgagc aattgaccac ttcgtacgat tcgagggaaa ctccgttgga atcgaaaagg
     2461 cctgaatggg ccgatggcga ttgtcgtaga acgcattgtt atacttgatg aactggagcg
     2521 tgcgataggc acgttctgcc gctgcgtttc tgcggacctg cgttgtcatc acatcctgga
     2581 tgtatgccgg cttgaagatc gaca
//
LOCUS       DAOSUG010000459         2596 bp    DNA     linear   ENV 10-JUN-2024
DEFINITION  MAG TPA_asm: Micropruina sp. isolate AMR_MDS_2195
            NODE_16064_length_2596_cov_4.018497, whole genome shotgun sequence.
ACCESSION   DAOSUG010000459 DAOSUG010000000
VERSION     DAOSUG010000459.1
DBLINK      BioProject: PRJNA925477
            BioSample: SAMN32864262
            Sequence Read Archive: SRR4236673
KEYWORDS    WGS; ENV; Metagenome Assembled Genome; MAG; Third Party Data; TPA;
            TPA:assembly.
SOURCE      Micropruina sp. (wastewater metagenome)
  ORGANISM  Micropruina sp.
            Bacteria; Bacillati; Actinomycetota; Actinomycetes;
            Propionibacteriales; Nocardioidaceae; Micropruina.
REFERENCE   1  (bases 1 to 2596)
  AUTHORS   Abdulkadir,N., Saraiva,J.P., Zhang,J., Stolte,S., Gillor,O.,
            Harms,H. and Rocha,U.
  TITLE     Genome-centric analyses of 165 metagenomes show that mobile genetic
            elements are crucial for the transmission of antimicrobial
            resistance genes to pathogens in activated sludge and wastewater
  JOURNAL   Microbiol Spectr 12 (3), e0291823 (2024)
   PUBMED   38289113
REFERENCE   2  (bases 1 to 2596)
  AUTHORS   Abdulkadir,N.
  TITLE     Direct Submission
  JOURNAL   Submitted (24-MAR-2023) Environmental Microbiology, Helmholtz
            Center for Environmental Research -UFZ, Permoserstr 15, Leipzig
            04318, Germany
COMMENT     The annotation was added by the NCBI Prokaryotic Genome Annotation
            Pipeline (PGAP). Information about PGAP can be found here:
            https://www.ncbi.nlm.nih.gov/genome/annotation_prok/
            
            ##Genome-Assembly-Data-START##
            Assembly Method        :: SPAdes v. 3.11.1
            Genome Representation  :: Full
            Expected Final Version :: Yes
            Genome Coverage        :: Not Applicable
            Sequencing Technology  :: Illumina MiSeq
            ##Genome-Assembly-Data-END##
            
            ##Genome-Annotation-Data-START##
            Annotation Provider               :: NCBI
            Annotation Date                   :: 05/04/2023 04:57:10
            Annotation Pipeline               :: NCBI Prokaryotic Genome
                                                 Annotation Pipeline (PGAP)
            Annotation Method                 :: Best-placed reference protein
                                                 set; GeneMarkS-2+
            Annotation Software revision      :: 6.5
            Features Annotated                :: Gene; CDS; rRNA; tRNA; ncRNA
            Genes (total)                     :: 2,776
            CDSs (total)                      :: 2,750
            Genes (coding)                    :: 2,714
            CDSs (with protein)               :: 2,714
            Genes (RNA)                       :: 26
            tRNAs                             :: 24
            ncRNAs                            :: 2
            Pseudo Genes (total)              :: 36
            CDSs (without protein)            :: 36
            Pseudo Genes (ambiguous residues) :: 0 of 36
            Pseudo Genes (frameshifted)       :: 2 of 36
            Pseudo Genes (incomplete)         :: 32 of 36
            Pseudo Genes (internal stop)      :: 2 of 36
            ##Genome-Annotation-Data-END##
FEATURES             Location/Qualifiers
     source          1..2596
                     /organism="Micropruina sp."
                     /mol_type="genomic DNA"
                     /submitter_seqid="NODE_16064_length_2596_cov_4.018497"
                     /isolate="AMR_MDS_2195"
                     /isolation_source="Activated sludge of municipal
                     wastewater treatment plants"
                     /db_xref="taxon:2737536"
                     /environmental_sample
                     /geo_loc_name="Canada"
                     /lat_lon="49.25 N 123.1 W"
                     /collection_date="2015-06-16"
                     /metagenome_source="wastewater metagenome"
                     /note="metagenomic"
     gene            <1..710
                     /locus_tag="PLF56_12695"
     CDS             <1..710
                     /locus_tag="PLF56_12695"
                     /inference="COORDINATES: ab initio
                     prediction:GeneMarkS-2+"
                     /note="Derived by automated computational analysis using
                     gene prediction method: GeneMarkS-2+."
                     /codon_start=3
                     /transl_table=11
                     /product="hypothetical protein"
                     /protein_id="HOQ54457.1"
                     /translation="TLAYQWSRAGAPIDGATATTYTPVAADLGKSLTVTATGTLAGRT
                     ATSKTSAAVTVSVGTLKSATPKLSGSVKVGSRVTAVPGAWTSGTRFSYQWKRSGSSIK
                     GATGSTYTPTSSDRGTTLSVSVTGSLAGHTSVTKASAGSKVGYGTLTSSTPKISGTVK
                     VGKKLSVVRGTWTSGTSFSYQWYANGKAIKGKKSSTLTLGTSQRGKKITVKVTGKKSG
                     YTTVSKASKATAKVAKK"
     gene            complement(816..2060)
                     /locus_tag="PLF56_12700"
     CDS             complement(816..2060)
                     /locus_tag="PLF56_12700"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_016455845.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="TRAM domain-containing protein"
                     /protein_id="HOQ54458.1"
                     /translation="MQPGDLIGPVEIGPVAHGGHCVARVDGRVVFVRHVLPGEVVRAR
                     VTEVSSRFARADAVEIVRSSPERMKPPCPHAGVCGGCDFQHVRPEFQAELKRRVLAEQ
                     LQRLAGIECNGVVEQVPPLLGSRVRMRFVATDDGRLGLRAHRSHVVVPLPVDGCRIAH
                     PLMPATVEGGEVAEAGSALGSWQARSTEEPLVGEAGAASGSRQARSAEEPQAEVIGVV
                     SLEGGRLVGAGSPGASEVITQRVGERTFSVNVNGFWQSHLAAPEVLTAAVLEGLAPSP
                     GETAFDLYCGVGVFAAALVDADCRVWGIEGSRTAVAHARRNVPQASFFAGDVGRTLRR
                     LPKQADLVVLDPPRTGAGAAVMTAVTRLRPRAIAYVACDPAALARDLGTARALGYEAS
                     SIRGFDVFGMTHHIEAVAVLRR"
     gene            complement(2062..>2596)
                     /locus_tag="PLF56_12705"
     CDS             complement(2062..>2596)
                     /locus_tag="PLF56_12705"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_011756341.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=2
                     /transl_table=11
                     /product="DNA-binding protein"
                     /protein_id="HOQ54459.1"
                     /translation="VEPEERNLLPSRVRAVIVVIGINKPTVRAVNFAKASRPSSMEAV
                     TVSVDADETKALLDDWYVEDLGVPLRVIASPYREVVSPILEFVKGIRSDNPRDVVCVY
                     IPEYVVGHWWEQLLHNQTALILKARLNFMPGVMVTSVPYLLASSKYAIDRAKRNPAAW
                     RSAGTGAVHSGQHLNSD"
BASE COUNT          450 a          953 c          852 g          341 t
ORIGIN      
        1 ccaccctggc ctaccagtgg tcacgtgcgg gcgcaccgat cgacggcgcc accgccacga
       61 cctacacccc ggtcgcggcc gacctcggca agtcgctcac cgtcaccgcg accggcaccc
      121 tggccgggcg cacggcgacc agcaagacct cggccgccgt cacggtgagc gtcggcacgc
      181 tgaaatcggc gacgccgaag ctctcgggtt cggtcaaggt gggcagcagg gtgaccgcgg
      241 tgccgggtgc gtggacctca ggcacgaggt tcagctacca gtggaagcgg tccggctcgt
      301 ccatcaaggg cgccaccggc tcgacctaca cgcccacctc cagtgaccgc ggcacgaccc
      361 tgtcggtgag cgtcacgggt tcactggccg gccacacctc ggtgacgaag gcctcggccg
      421 gcagcaaggt gggctatggc acccttacgt ccagcacccc gaagatctcg ggcaccgtca
      481 aggtgggcaa gaagctcagc gtggtgcgcg gcacctggac ctcgggcacg tcgttcagct
      541 accagtggta cgccaacggc aaggcgatca agggcaagaa gtcctcgacg ctgaccctgg
      601 gcacgtcgca gcggggcaag aagatcaccg tcaaggtgac cggcaagaag tcgggctaca
      661 ccacggtgag caaggcctcg aaggccaccg cgaaggtcgc caagaagtag ctcggatcgg
      721 acggcggtcg cggcttcggc tgcggccgcc gttcggcatg agtgggtgac gcgcgagacg
      781 cgtccgacga cgctcaggga cgggtgcggg ggtggtcacc gccgcaggac tgcgaccgcc
      841 tcgatgtggt gggtcatgcc gaacacatcg aaaccgcgaa tggacgaggc ctcgtacccc
      901 aatgcccgcg ccgtccccag atcgcgagcc agcgccgccg gatcgcaggc cacgtatgcg
      961 atcgcccggg gacgcagccg ggtcacggca gtcatcaccg ccgcgccggc accggttcgg
     1021 ggcggatcga gcaccaccag atcggcttgc ttcggcagcc ggcgaagggt ccgcccgaca
     1081 tcgccggcga agaaggacgc ctgcggcacg ttacgccggg cgtgcgcgac cgccgtccgg
     1141 ctgccctcga tgccccacac tcggcagtcg gcgtcgacca gggctgcggc gaagacgccc
     1201 acgccgcagt acaggtcgaa cgcggtctcg ccgggcgagg gtgcgaggcc ctccagcact
     1261 gcggcggtga gcacctcggg cgccgccagg tgcgactgcc agaacccgtt cacgttcacc
     1321 gagaacgtgc gctcgcccac ccgctgggtg atcacctcgg acgccccggg cgaccccgcc
     1381 cccaccagtc gcccgccttc gaggctgacc acaccgatca cctcagcctg cggttcctcc
     1441 gccgatcgag cctgccgaga gccggaggcg gccccagcct ccccgaccag cggctcctcc
     1501 gtcgatcgcg cctgccaaga cccgagagcg gacccagcct cggccacctc accgccctcg
     1561 accgttgcgg gcatcaacgg gtgggcgatc cggcagccgt ccacaggtag tggcaccacg
     1621 acgtgcgagc ggtgggcacg caggcccagg cgcccgtcgt cggtggccac gaaccgcatc
     1681 cgcacccgcg agccgagcag cggcggcacc tgctcgacca ccccgttgca ctcgatgccg
     1741 gccagacgct gcagttgttc ggccagcacc cgacgcttca actcggcctg aaactcgggc
     1801 cgcacgtgct gaaagtcgca gccgccgcag acccccgcat gcgggcaggg cggtttcatg
     1861 cgctccggcg aagaccggac gatctcgacg gcgtccgcgc gggcgaaacg actgctcacc
     1921 tccgtcaccc gcgcccgcac cacctcgccg ggcagcacgt gccgcacgaa caccacgcgt
     1981 ccgtccaccc tcgccacaca atgcccgccg tgggcgacgg ggccgatctc gaccgggccg
     2041 atcaggtcac cgggctgcac gtcagtccga gttcaggtgc tggccggaat ggacggcacc
     2101 ggtaccggcg ctgcgccagg ccgcgggatt gcgtttggcc cggtcgatcg cgtacttgga
     2161 cgaggccagc aggtacggca ccgaggtgac catcacaccc ggcatgaagt tgagccgagc
     2221 cttgagaatc agcgcggtct ggttgtgcag caactgttcc caccagtgac ccacgacgta
     2281 ttcgggaatg tagacgcaca ccacgtcgcg cgggttgtcg gagcgaatgc ccttgacgaa
     2341 ctcgagaatc ggtgacacca cctcgcgata cggcgacgcg atcacccgta ggggcacccc
     2401 tagatcctcc acgtaccaat cgtccaacag ggccttggtt tcgtcggcgt ccaccgagac
     2461 ggtcacggcc tccatcgaac tgggtcgcga cgccttagcg aagttcaccg cccgtaccgt
     2521 cggcttgttg atgccgatca ccacgatcac cgcgcgcacc cggctgggca gcagattgcg
     2581 ctcctcgggt tcgacg
//
LOCUS       DAOSUG010000460         2591 bp    DNA     linear   ENV 10-JUN-2024
DEFINITION  MAG TPA_asm: Micropruina sp. isolate AMR_MDS_2195
            NODE_16112_length_2591_cov_4.725946, whole genome shotgun sequence.
ACCESSION   DAOSUG010000460 DAOSUG010000000
VERSION     DAOSUG010000460.1
DBLINK      BioProject: PRJNA925477
            BioSample: SAMN32864262
            Sequence Read Archive: SRR4236673
KEYWORDS    WGS; ENV; Metagenome Assembled Genome; MAG; Third Party Data; TPA;
            TPA:assembly.
SOURCE      Micropruina sp. (wastewater metagenome)
  ORGANISM  Micropruina sp.
            Bacteria; Bacillati; Actinomycetota; Actinomycetes;
            Propionibacteriales; Nocardioidaceae; Micropruina.
REFERENCE   1  (bases 1 to 2591)
  AUTHORS   Abdulkadir,N., Saraiva,J.P., Zhang,J., Stolte,S., Gillor,O.,
            Harms,H. and Rocha,U.
  TITLE     Genome-centric analyses of 165 metagenomes show that mobile genetic
            elements are crucial for the transmission of antimicrobial
            resistance genes to pathogens in activated sludge and wastewater
  JOURNAL   Microbiol Spectr 12 (3), e0291823 (2024)
   PUBMED   38289113
REFERENCE   2  (bases 1 to 2591)
  AUTHORS   Abdulkadir,N.
  TITLE     Direct Submission
  JOURNAL   Submitted (24-MAR-2023) Environmental Microbiology, Helmholtz
            Center for Environmental Research -UFZ, Permoserstr 15, Leipzig
            04318, Germany
COMMENT     The annotation was added by the NCBI Prokaryotic Genome Annotation
            Pipeline (PGAP). Information about PGAP can be found here:
            https://www.ncbi.nlm.nih.gov/genome/annotation_prok/
            
            ##Genome-Assembly-Data-START##
            Assembly Method        :: SPAdes v. 3.11.1
            Genome Representation  :: Full
            Expected Final Version :: Yes
            Genome Coverage        :: Not Applicable
            Sequencing Technology  :: Illumina MiSeq
            ##Genome-Assembly-Data-END##
            
            ##Genome-Annotation-Data-START##
            Annotation Provider               :: NCBI
            Annotation Date                   :: 05/04/2023 04:57:10
            Annotation Pipeline               :: NCBI Prokaryotic Genome
                                                 Annotation Pipeline (PGAP)
            Annotation Method                 :: Best-placed reference protein
                                                 set; GeneMarkS-2+
            Annotation Software revision      :: 6.5
            Features Annotated                :: Gene; CDS; rRNA; tRNA; ncRNA
            Genes (total)                     :: 2,776
            CDSs (total)                      :: 2,750
            Genes (coding)                    :: 2,714
            CDSs (with protein)               :: 2,714
            Genes (RNA)                       :: 26
            tRNAs                             :: 24
            ncRNAs                            :: 2
            Pseudo Genes (total)              :: 36
            CDSs (without protein)            :: 36
            Pseudo Genes (ambiguous residues) :: 0 of 36
            Pseudo Genes (frameshifted)       :: 2 of 36
            Pseudo Genes (incomplete)         :: 32 of 36
            Pseudo Genes (internal stop)      :: 2 of 36
            ##Genome-Annotation-Data-END##
FEATURES             Location/Qualifiers
     source          1..2591
                     /organism="Micropruina sp."
                     /mol_type="genomic DNA"
                     /submitter_seqid="NODE_16112_length_2591_cov_4.725946"
                     /isolate="AMR_MDS_2195"
                     /isolation_source="Activated sludge of municipal
                     wastewater treatment plants"
                     /db_xref="taxon:2737536"
                     /environmental_sample
                     /geo_loc_name="Canada"
                     /lat_lon="49.25 N 123.1 W"
                     /collection_date="2015-06-16"
                     /metagenome_source="wastewater metagenome"
                     /note="metagenomic"
     gene            complement(105..1064)
                     /locus_tag="PLF56_12710"
     CDS             complement(105..1064)
                     /locus_tag="PLF56_12710"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_013864741.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="nucleoside hydrolase"
                     /protein_id="HOQ54460.1"
                     /translation="MSPRPVYFDCDTGIDDALALVYLMASPEITLAGIGTVSGNIDAA
                     QAAVNTLDLLDLGGRREVPVAVGAMDPLTRAFTGGVPHIHGRNGVGNVELPHTERRPV
                     DESAADMLVRLAHEFDGELDVIAIGPLTNLALALAQDPSIAGKVHEVVVMGGAALVPG
                     NLSPVGEANIWNDPEAAQAVMAAPWPVVLVPLDVTLENVFEESDRLALAASDSALNRA
                     LADILDLYFGFYVAEYGRKLCALHDPLAAAIAVGQIRATRAPSVPVVVDDTQGPGRGQ
                     TICDLRGQRNGPVDVEGAHVRVVLDTDQPLGPVLLKRLLDYRA"
     gene            complement(1088..2311)
                     /locus_tag="PLF56_12715"
     CDS             complement(1088..2311)
                     /locus_tag="PLF56_12715"
                     /inference="COORDINATES: protein motif:HMM:TIGR00804.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_component: GO:0005886 - plasma membrane [Evidence IEA];
                     GO_function: GO:0005337 - nucleoside transmembrane
                     transporter activity [Evidence IEA];
                     GO_function: GO:0015293 - symporter activity [Evidence
                     IEA];
                     GO_process: GO:1901642 - nucleoside transmembrane
                     transport [Evidence IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="NupC/NupG family nucleoside CNT transporter"
                     /protein_id="HOQ54461.1"
                     /translation="MDRFQGVLGIVAILGLVVALSRFRTRINWRILGAGFALQVVTAL
                     LVLKWEPGFKALEALSAGVSTLIGYADEGTKFVFGALLDQDKIGFVFALSVLPVIIFI
                     GALIGLLYYLRVIQWFIEIVGTAMKWVVGVSKIESVWATTVIFLGMSEAPLVIAPYLK
                     KLTKSALFTCMVGGFASVAGSTLVGYSLLGAPLPYLIAASVMNAPASLVIAKGLWPET
                     EESDLDGSVRDVKDTESANVIDALARGALSGGHLAVIVGCLLIAFIATIALANGLVSG
                     VAGWFGHPEITFDKIFGWIFAPVAWLIGVPWNEAPTVGNLIGQKTVLNEFVAYSTLGP
                     MVTANTLSSKSVVISTFALAGFANFGSIAIMIGSLGSLVPERRGEIARNGLFALLGGT
                     LANLLNASIAGVVAG"
BASE COUNT          444 a          878 c          883 g          386 t
ORIGIN      
        1 cgggatgacg aagtgcacgg tgcccgggat gacgaagtgc acggcacccg ggatgacgag
       61 tgcgccactg atcgagaccc ccgtcgggca atggtgtcga ctggtcaggc ccggtagtcg
      121 agcaggcgct tcagcagcac cggcccgagt ggctgatccg tgtcgagcac cacgcgcacg
      181 tgggcgccct cgacgtccac gggtccgttg cgctgccctc gcaggtcaca gatggtctgg
      241 ccgcgtccgg ggccctgggt gtcgtccacc accaccggca cggacggtgc ccgcgtcgcg
      301 cggatctgcc ccacggcgat ggccgcggcc agcgggtcgt gcagggcgca cagcttgcgt
      361 ccgtattcgg cgacgtagaa gccgaagtac aggtcgagaa tgtcggccag tgcccggttc
      421 aaggctgagt cggacgccgc cagcgccagc cggtcggact cttcgaacac gttctcgagc
      481 gtgacgtcca gcggcaccag caccacgggc cagggcgccg ccatcaccgc ctgcgccgcc
      541 tcgggatcgt tccagatgtt ggcctcgccg accgggctca aattgccggg caccagggcc
      601 gccccgccca tcacgacgac ctcatgcacc ttgccggcga tggacggatc ctgcgccagc
      661 gccagcgcca gattggtcag ggggccgatc gcgatcacgt ccaactcgcc gtcgaactcg
      721 tgcgccagcc ggaccagcat gtcggctgcc gactcgtcga ccggccgtcg ctcggtgtgc
      781 ggcaactcga cgttgccgac cccgttgcgt ccgtgaatgt gcgggacgcc gccggtgaag
      841 gccctggtca gcgggtccat cgcgccaacc gcgaccggca cctcgcgccg gccgcccagg
      901 tcgagcaggt cgagggtgtt gaccgccgcc tgcgcggcgt cgatgttgcc gctgaccgta
      961 ccgatgccgg cgagcgtgat ctcgggcgag gccatcaggt agaccaacgc caatgcgtcg
     1021 tcgatgccgg tgtcacagtc gaagtagacg gggcgggggc tcatcgggga cgacctctct
     1081 gtcgggctca gccggccacg acgccggcga tggatgcgtt cagcaggttg gccaacgtac
     1141 cgcccagcag cgcgaagagg ccgttgcggg cgatctcgcc gcgccgctcg ggtaccaggc
     1201 tgcccagcga gccgatcatg atggcgatcg aaccgaagtt cgcgaagccg gccagcgcga
     1261 aggtgctgat caccacgctc ttcgagctga gggtgttggc ggtgaccatc gggccgagcg
     1321 tgctgtaggc gacgaactcg ttgagcaccg tcttctggcc gatcaggttg ccaacggtcg
     1381 gagcctcgtt ccagggcacc ccgatcagcc aggcgaccgg ggcgaagatc cacccgaaga
     1441 tcttgtcgaa ggtgatctcg gggtgcccga accagcccgc gaccccgctg accaggccgt
     1501 tggcgagtgc gatggtggca atgaacgcga tcagcaggca gccgacgatc accgccaggt
     1561 gcccgccgga cagtgcgccc cgggccagcg cgtcgatcac attggccgac tcggtgtcct
     1621 tgacgtcgcg gacggagccg tcgaggtcac tctcttcggt ctcgggccac aggcccttcg
     1681 cgatcaccag ggacgcgggc gcgttcatca ccgaggccgc gatcaggtag ggcagcgggg
     1741 caccgagcag cgagtagccg accagcgtcg agccggccac cgacgcgaaa cccccgacca
     1801 tgcaggtgaa cagtgccgac ttggtgagct tcttcaggta gggcgcaatc accagcggcg
     1861 cctcgctcat gcccaggaag atgacggtgg tcgcccagac cgattcgatc ttgctgaccc
     1921 cgaccaccca tttcatggcc gtgccgacga tctcgatgaa ccactggatc acccgcaggt
     1981 agtacaacag cccgatcagg gcaccgatga agatgatcac cggcagcacc gacagggcga
     2041 agacgaagcc gatcttgtcc tgatcgagca gggcgccgaa cacgaacttg gtgccctcgt
     2101 ccgcatagcc gatcagggtg gacaccccgg ccgagagcgc ctccagcgcc ttgaagccgg
     2161 gttcccactt gagcaccagc agagcggtga caacctgcag ggcgaagcct gccccgagta
     2221 ttcgccagtt gattcgggtg cgaaacctcg agagggcgac caccagcccc aggatcgcaa
     2281 cgatgcccaa aacgccctgg aaccggtcca tgccagcctc ctcgcaacgg atgcttggcg
     2341 accctattcg gattgattcg gccctggcaa gggtgttccg cacgaggtgg ctctccgagc
     2401 gaggatctgc cagtcgccgg gagagattgg ggagggttct ggaccgtgct gggaaccgaa
     2461 ccgtgccggg acggtgctgg gtcccgtccc cgaccagcgc gcagcacgag ggcccggacg
     2521 gttgctccgt ccgggccctc gtggtgggtc agcgcaggtc gtccgggttc tgggtgggac
     2581 gctccgccgg c
//
LOCUS       DAOSUG010000461         2590 bp    DNA     linear   ENV 10-JUN-2024
DEFINITION  MAG TPA_asm: Micropruina sp. isolate AMR_MDS_2195
            NODE_16124_length_2590_cov_4.553846, whole genome shotgun sequence.
ACCESSION   DAOSUG010000461 DAOSUG010000000
VERSION     DAOSUG010000461.1
DBLINK      BioProject: PRJNA925477
            BioSample: SAMN32864262
            Sequence Read Archive: SRR4236673
KEYWORDS    WGS; ENV; Metagenome Assembled Genome; MAG; Third Party Data; TPA;
            TPA:assembly.
SOURCE      Micropruina sp. (wastewater metagenome)
  ORGANISM  Micropruina sp.
            Bacteria; Bacillati; Actinomycetota; Actinomycetes;
            Propionibacteriales; Nocardioidaceae; Micropruina.
REFERENCE   1  (bases 1 to 2590)
  AUTHORS   Abdulkadir,N., Saraiva,J.P., Zhang,J., Stolte,S., Gillor,O.,
            Harms,H. and Rocha,U.
  TITLE     Genome-centric analyses of 165 metagenomes show that mobile genetic
            elements are crucial for the transmission of antimicrobial
            resistance genes to pathogens in activated sludge and wastewater
  JOURNAL   Microbiol Spectr 12 (3), e0291823 (2024)
   PUBMED   38289113
REFERENCE   2  (bases 1 to 2590)
  AUTHORS   Abdulkadir,N.
  TITLE     Direct Submission
  JOURNAL   Submitted (24-MAR-2023) Environmental Microbiology, Helmholtz
            Center for Environmental Research -UFZ, Permoserstr 15, Leipzig
            04318, Germany
COMMENT     The annotation was added by the NCBI Prokaryotic Genome Annotation
            Pipeline (PGAP). Information about PGAP can be found here:
            https://www.ncbi.nlm.nih.gov/genome/annotation_prok/
            
            ##Genome-Assembly-Data-START##
            Assembly Method        :: SPAdes v. 3.11.1
            Genome Representation  :: Full
            Expected Final Version :: Yes
            Genome Coverage        :: Not Applicable
            Sequencing Technology  :: Illumina MiSeq
            ##Genome-Assembly-Data-END##
            
            ##Genome-Annotation-Data-START##
            Annotation Provider               :: NCBI
            Annotation Date                   :: 05/04/2023 04:57:10
            Annotation Pipeline               :: NCBI Prokaryotic Genome
                                                 Annotation Pipeline (PGAP)
            Annotation Method                 :: Best-placed reference protein
                                                 set; GeneMarkS-2+
            Annotation Software revision      :: 6.5
            Features Annotated                :: Gene; CDS; rRNA; tRNA; ncRNA
            Genes (total)                     :: 2,776
            CDSs (total)                      :: 2,750
            Genes (coding)                    :: 2,714
            CDSs (with protein)               :: 2,714
            Genes (RNA)                       :: 26
            tRNAs                             :: 24
            ncRNAs                            :: 2
            Pseudo Genes (total)              :: 36
            CDSs (without protein)            :: 36
            Pseudo Genes (ambiguous residues) :: 0 of 36
            Pseudo Genes (frameshifted)       :: 2 of 36
            Pseudo Genes (incomplete)         :: 32 of 36
            Pseudo Genes (internal stop)      :: 2 of 36
            ##Genome-Annotation-Data-END##
FEATURES             Location/Qualifiers
     source          1..2590
                     /organism="Micropruina sp."
                     /mol_type="genomic DNA"
                     /submitter_seqid="NODE_16124_length_2590_cov_4.553846"
                     /isolate="AMR_MDS_2195"
                     /isolation_source="Activated sludge of municipal
                     wastewater treatment plants"
                     /db_xref="taxon:2737536"
                     /environmental_sample
                     /geo_loc_name="Canada"
                     /lat_lon="49.25 N 123.1 W"
                     /collection_date="2015-06-16"
                     /metagenome_source="wastewater metagenome"
                     /note="metagenomic"
     gene            complement(<1..480)
                     /locus_tag="PLF56_12720"
     CDS             complement(<1..480)
                     /locus_tag="PLF56_12720"
                     /EC_number="2.3.1.286"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_017936814.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_function: GO:0070403 - NAD+ binding [Evidence IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="Sir2 family NAD-dependent protein deacetylase"
                     /protein_id="HOQ54462.1"
                     /translation="MSAAHGGTGLKPGVVHSTAASASMDALAALLRARRWVALTGAGI
                     STDSGIPDYRGADARPTSPIQYDDFVRFADVRRRYWARSLLGFRSFGVARPNAGHLAL
                     AALTEQGLGTVITQNVDGLHQAAGSPSVIDLHGRIDRVVCLACGEVSPRVRLQHLLEA
                     "
     gene            complement(477..1463)
                     /locus_tag="PLF56_12725"
     CDS             complement(477..1463)
                     /locus_tag="PLF56_12725"
                     /EC_number="3.4.21.-"
                     /inference="COORDINATES: protein motif:HMM:NF013827.2"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_function: GO:0004252 - serine-type endopeptidase
                     activity [Evidence IEA];
                     GO_function: GO:0008270 - zinc ion binding [Evidence IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="rhomboid family intramembrane serine protease"
                     /protein_id="HOQ54463.1"
                     /translation="MSQPPSFQPPAQPPWGPYPPGGQQPPVQVCYRHPDRATLIACQR
                     CRRPICPECMVQAHVGVQCPECVRAGARATRSDEGPFGGVRSANPRLTSLVLIGVNLA
                     VFVLIVLTGGGFGRWATRLAITPLGNCLVDDGTGRYYPGLGETACNAVPGAFWADGVA
                     TGGWWQVITSAFAHAELTHVAMNLLALYFLGPPLEQVLGRARFLAVYFVSALAGSAAV
                     MWLAAPDSSALGASGAIFGLLGALLLIARRSGGNYQLIAMWLGINVVITVLGGSYISW
                     QGHLGGLIGGLAVAAALVLPPRTQRARWQWPGVIALTVLLLVAIAVRAMMLR"
     gene            complement(1469..2488)
                     /locus_tag="PLF56_12730"
     CDS             complement(1469..2488)
                     /locus_tag="PLF56_12730"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_012920162.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="aldo/keto reductase"
                     /protein_id="HOQ54464.1"
                     /translation="MEHRLLGDSGLAVSRLCLGTMTFGDETDESGSFEQLDAFVEAGG
                     TLIDTADVYSRGVSEQIIGAWLAQRDGVREQVVLATKGRFPMNESPNGLGLSRRHLHD
                     ALNASLRRLGVDSIDLYQLHAWDPLTPVDEYLRFIDDAVRAGKVHYFGLSNFLGWQLT
                     AVAERALASGRTAPVSLQPQYNLLTRGIEPEIVPACAQYGLGLMPWSPLAGGWLTGKY
                     RRDARPTGSSRLGENPSRGMEAYDRRNTEATWRVLDELQQVAEQSGLTMAQAALAWVA
                     DRPQVTAPIIGARTLEQLQGSLAIAEVHLDHAAARRLTDVSAPQLADYPYGELGIDQR
                     NRSVS"
BASE COUNT          405 a          922 c          920 g          343 t
ORIGIN      
        1 cgcctccagc aggtgctgca gccgaacgcg cggggacacc tcaccgcagg ccaggcagac
       61 cacccggtcg atccggccgt gcagatcgat cacggacggg ctgccggcgg cctggtgcag
      121 cccgtccacg ttctgcgtga tcaccgtgcc caacccctgc tcggtcaacg cggcgagcgc
      181 cagatggccg gcgttgggcc tcgccacccc gaaactgcga aagcccagca gtgagcgcgc
      241 ccagtagcgc cgccgcacgt cggcgaagcg cacgaagtcg tcgtactgga tggggctggt
      301 cggacgagcg tccgccccgc ggtagtccgg aatgcccgag tcggtgctga tgcccgcccc
      361 ggtcagcgcc acccagcgcc gtgcgcgcag cagggcggcc agcgcgtcca tgctggccga
      421 tgccgcggtg ctgtgcacaa cgcccggctt gagacccgtc ccgccgtggg ccgcgctcac
      481 cgcagcatca tcgcccggac ggcgatcgcc accaacagca gcacggtgag agcgatcacc
      541 ccgggccact gccaacgggc ccgctgcgtg cgcggcggta gcaccagcgc ggccgcgacc
      601 gccagcccgc cgatcagccc gcccaggtgg ccctgccacg agatgtacga gccgccgagc
      661 acggtgatca ccacattgat gcccagccac atggcgatca gctggtagtt gccgcccgat
      721 ctgcgcgcga tcaacagcag cgcgcccagc aggccgaaga tggcacccga ggcgcccagg
      781 gccgagctgt cgggtgcggc cagccacatc accgccgccg aaccggccaa cgcgctgacg
      841 aagtagacgg ccagaaagcg agcccgtccg agcacctgtt cgagcggcgg gcccagaaag
      901 tacaaggcga gcaggttcat cgccacgtgc gtcagctcgg cgtgcgcgaa ggcgctcgtg
      961 atcacctgcc accacccgcc ggtcgcgacc ccgtcggccc agaaagcgcc cgggacggcg
     1021 ttgcaggccg tttcgccaag gcccgggtag tagcgtccgg tgccgtcatc gaccaggcag
     1081 ttgcccagcg gcgtgatggc caaccgggtc gcccagcgtc cgaacccgcc gccggtgaga
     1141 acgatcagca cgaacaccgc caggttgacg ccgatcagca ccagtgaggt gagccgtgga
     1201 ttggccgacc ggacgccccc gaacgggccc tcgtcgctgc gcgtggccct cgctcccgca
     1261 cggacgcact ccggacactg cacgcccacg tgcgcctgca ccatgcattc gggacagatc
     1321 ggccgacggc accgctggca cgcgatgagt gtggcgcggt cgggatgccg ataacagacc
     1381 tgcaccggtg gttgctggcc ccccggcggg tacgggcccc acgggggctg cgcgggcggc
     1441 tggaacgagg gcggctggct catcgggctc aggacaccga gcgattgcgc tggtcgatgc
     1501 ccaactcgcc gtaggggtag tcggccaact gcggtgcgga tacgtcggtg agccgtcggg
     1561 cggcggcgtg atcgaggtgc acctcggcga tcgccagcga gccctgcaac tgctccaggg
     1621 tgcgggcgcc gatgatcggc gcggtcacct gcggacggtc ggcgacccag gccagcgccg
     1681 cctgcgccat ggtcagcccg gactgctccg cgacctgctg cagctcgtcc agcacccgcc
     1741 aggtggcctc ggtgttgcgg cggtcgtagg cctccatgcc gcgggacggg ttctcgccca
     1801 gccggctgct gccggtggga cgcgcgtcgc ggcggtactt gccggtcaac cacccgccgg
     1861 ccagggggct ccacggcatc aggcccagcc cgtactgcgc gcacgcgggc acgatctcgg
     1921 gttcgatgcc gcgggtgagc aggttgtact gcggctgcag cgacaccggc gccgtccgcc
     1981 ccgaggcgag agcgcgctcg gccaccgcgg tcagttgcca gccgagaaag ttggacagcc
     2041 cgaagtagtg caccttgccc gcacgcaccg cgtcgtcgat gaagcgcagg tactcatcga
     2101 cgggggtcaa cgggtcccag gcgtgcagct ggtacaggtc gatcgagtcg acgccgagtc
     2161 ggcgcaggct ggcgttcagt gcatcgtgca ggtggcggcg cgacaagccg aggccgttgg
     2221 gcgactcgtt catcgggaac cggcctttgg tggcaagcac cacctgctcg cggacgccgt
     2281 cgcgctgcgc cagccacgcg ccgatgatct gttcggagac gccgcgcgag tagacgtccg
     2341 cggtgtcgat gagagtgccg ccggcctcga cgaatgcgtc caactgctcg aaggagccgg
     2401 actcatcggt ttcgtcgccg aaggtcatgg tgcccaggca caggcgtgaa acggccagcc
     2461 cgctgtcgcc gagaagtcgg tgttccatcc ctccacccta atcgtggatc cgggcggggc
     2521 tgatggacga gcgggcgggg ggcttgtgcg ggtgcgtggg tgctgcgctc gctaggctga
     2581 ccgggtgccg
//
LOCUS       DAOSUG010000462         2585 bp    DNA     linear   ENV 10-JUN-2024
DEFINITION  MAG TPA_asm: Micropruina sp. isolate AMR_MDS_2195
            NODE_16167_length_2585_cov_4.114229, whole genome shotgun sequence.
ACCESSION   DAOSUG010000462 DAOSUG010000000
VERSION     DAOSUG010000462.1
DBLINK      BioProject: PRJNA925477
            BioSample: SAMN32864262
            Sequence Read Archive: SRR4236673
KEYWORDS    WGS; ENV; Metagenome Assembled Genome; MAG; Third Party Data; TPA;
            TPA:assembly.
SOURCE      Micropruina sp. (wastewater metagenome)
  ORGANISM  Micropruina sp.
            Bacteria; Bacillati; Actinomycetota; Actinomycetes;
            Propionibacteriales; Nocardioidaceae; Micropruina.
REFERENCE   1  (bases 1 to 2585)
  AUTHORS   Abdulkadir,N., Saraiva,J.P., Zhang,J., Stolte,S., Gillor,O.,
            Harms,H. and Rocha,U.
  TITLE     Genome-centric analyses of 165 metagenomes show that mobile genetic
            elements are crucial for the transmission of antimicrobial
            resistance genes to pathogens in activated sludge and wastewater
  JOURNAL   Microbiol Spectr 12 (3), e0291823 (2024)
   PUBMED   38289113
REFERENCE   2  (bases 1 to 2585)
  AUTHORS   Abdulkadir,N.
  TITLE     Direct Submission
  JOURNAL   Submitted (24-MAR-2023) Environmental Microbiology, Helmholtz
            Center for Environmental Research -UFZ, Permoserstr 15, Leipzig
            04318, Germany
COMMENT     The annotation was added by the NCBI Prokaryotic Genome Annotation
            Pipeline (PGAP). Information about PGAP can be found here:
            https://www.ncbi.nlm.nih.gov/genome/annotation_prok/
            
            ##Genome-Assembly-Data-START##
            Assembly Method        :: SPAdes v. 3.11.1
            Genome Representation  :: Full
            Expected Final Version :: Yes
            Genome Coverage        :: Not Applicable
            Sequencing Technology  :: Illumina MiSeq
            ##Genome-Assembly-Data-END##
            
            ##Genome-Annotation-Data-START##
            Annotation Provider               :: NCBI
            Annotation Date                   :: 05/04/2023 04:57:10
            Annotation Pipeline               :: NCBI Prokaryotic Genome
                                                 Annotation Pipeline (PGAP)
            Annotation Method                 :: Best-placed reference protein
                                                 set; GeneMarkS-2+
            Annotation Software revision      :: 6.5
            Features Annotated                :: Gene; CDS; rRNA; tRNA; ncRNA
            Genes (total)                     :: 2,776
            CDSs (total)                      :: 2,750
            Genes (coding)                    :: 2,714
            CDSs (with protein)               :: 2,714
            Genes (RNA)                       :: 26
            tRNAs                             :: 24
            ncRNAs                            :: 2
            Pseudo Genes (total)              :: 36
            CDSs (without protein)            :: 36
            Pseudo Genes (ambiguous residues) :: 0 of 36
            Pseudo Genes (frameshifted)       :: 2 of 36
            Pseudo Genes (incomplete)         :: 32 of 36
            Pseudo Genes (internal stop)      :: 2 of 36
            ##Genome-Annotation-Data-END##
FEATURES             Location/Qualifiers
     source          1..2585
                     /organism="Micropruina sp."
                     /mol_type="genomic DNA"
                     /submitter_seqid="NODE_16167_length_2585_cov_4.114229"
                     /isolate="AMR_MDS_2195"
                     /isolation_source="Activated sludge of municipal
                     wastewater treatment plants"
                     /db_xref="taxon:2737536"
                     /environmental_sample
                     /geo_loc_name="Canada"
                     /lat_lon="49.25 N 123.1 W"
                     /collection_date="2015-06-16"
                     /metagenome_source="wastewater metagenome"
                     /note="metagenomic"
     gene            complement(351..2039)
                     /locus_tag="PLF56_12735"
     CDS             complement(351..2039)
                     /locus_tag="PLF56_12735"
                     /inference="COORDINATES: ab initio
                     prediction:GeneMarkS-2+"
                     /note="Derived by automated computational analysis using
                     gene prediction method: GeneMarkS-2+."
                     /codon_start=1
                     /transl_table=11
                     /product="hypothetical protein"
                     /protein_id="HOQ54465.1"
                     /translation="MATASSARRAWIALSILLPALVVGVLTWLRRWVSDDGFINVRVI
                     LQLVAGNGPTYNLNERVEVGTSTLWLFFVTLGKLAAPWAEVGEVMVWLGTGFITLAML
                     LLGLGAMHLVPPRAGVVLPLGTAALAGLAPFWDFGTSGLETSLTFCWIAACFWALARR
                     WANRPRQALPAWRPWPVAMLIGLGVLIRPDLALVSLVFGVMLLCQSERRVKGWLAAAG
                     VALALPVAYEIFRMGYYASLVPNTALAKANGSVAQGLLYLRDFVLTYHLYLPAAFALA
                     LYAVGIVRGFGDRDPGLVALWLGPLVGVAIHAGFVISVGGDFMHARFLLPATVLLFAP
                     VAVIGGAAWTRVLAVLTMVWALVAGLALRPALWNGMIVDERSYYSAFVPDRPGAMVIA
                     ENWAADVGFQLTRQAARDLADGRSYFVDITKPDDKLPTYNGEGVYVVINSLGIAGVGA
                     GLKVVMIDPPSLGDPIGSRLVLPPDAKYRVGHAYKPVVWAMARYTASDVYPQDQALND
                     ARATLACGDIPTLISAVSEPLTFQRFMTNLAVAPKLTILRVPVDPAEARAHLCG"
     gene            2193..>2585
                     /locus_tag="PLF56_12740"
     CDS             2193..>2585
                     /locus_tag="PLF56_12740"
                     /inference="COORDINATES: protein motif:HMM:NF024118.2"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="MBL fold metallo-hydrolase"
                     /protein_id="HOQ54466.1"
                     /translation="MDVLLLGTGASDGVPNCWCRCPTCAEARASGAIRTNASALVDGR
                     LLIDCGPEAPRQAQRAGRDLTGVRSVLISHAHDDHLDPSFLMHRGWVSEQPLRVIGPA
                     PAIERCRDWLAPDQSVVSLTVVTAGDSLE"
BASE COUNT          448 a          900 c          897 g          340 t
ORIGIN      
        1 cgaacccccg ccttactttc cagggccagc gcgaagcttc gcgggcccgt cgttcgagac
       61 ccgtcccgtt ggccgagcct gtcgagaccc gtcatgttgg ccgagcttgt cgagacccgt
      121 cccggtggcc gagcttgtcg agacccgtcc cgttgatcgc tggtgccgcg ccatcggact
      181 cagcgatctc gagatcgccc gatgatttcg gcgacgccgc tcactttcgg gttgcagccc
      241 gaaaccgtgc agccaacccg aaaccgtgca gcgatctcga gatcgccgca ccgtccgctg
      301 gctccatcta cccagtcggc agagggaagc cggcagaggg aagccgtgag tcagccgcag
      361 aggtgcgcgc gggcctcggc aggatccacc ggaacacgca ggatggtcag cttgggcgcc
      421 accgccaggt tggtcatgaa ccgctggaag gtcaacggct ccgagactgc gctgatcagg
      481 gtcggaatgt caccgcaggc cagggtcgcc cgggcatcgt tcaacgcctg atcctgcggg
      541 tacacatcgg acgcggtgta gcgggccatg gcccacacca ccggcttgta ggcgtgcccc
      601 acccggtact tcgcatcggg cggcagcacc aggcgtgaac cgatcgggtc gcccaatgag
      661 ggcgggtcga tcatcaccac cttcaggcct gccccgacgc cggcgatacc gagggagttg
      721 atcaccacgt acacgccctc accgttgtag gtgggcagct tgtcgtccgg tttggtgatg
      781 tcgacgaagt aggaccgtcc atcggcgagg tcgcgggcgg cctgccgggt gagctgaaag
      841 ccgacgtcgg cggcccagtt ctcggcgatc accatcgccc ccgggcggtc gggcacgaac
      901 gcggagtagt agctgcgttc gtccacgatc atgccgttcc acaaggccgg acgcagcgcc
      961 agcccggcca ccagtgccca caccatggtg agcacggcga gtacccgggt ccaggcggcg
     1021 cccccgatca ccgcgaccgg cgcgaacagc agcacggtgg ccggtagcag aaagcgggcg
     1081 tgcatgaagt cgccgccgac cgagatcacg aagcccgcat ggatcgcgac ccccaccagg
     1141 gggccgagcc agagcgccac caggccgggg tcgcgatcgc cgaacccccg gacgatgccc
     1201 accgcgtaga gggccagcgc gaacgccgcc ggcaggtaca ggtggtaggt cagcacgaag
     1261 tcacgcaggt acagcagccc ctgggccacc gagccgttgg ccttggcgag cgcggtgttg
     1321 ggcaccagcg aggcgtagta gcccatgcgg aagatctcgt acgccaccgg cagcgccagc
     1381 gcgacgcccg cggccgcgag ccagcccttg accctgcgct cggactggca cagcagcatc
     1441 acgccgaaca ccaggctgac cagcgccagg tcgggccgga tcagcacccc cagaccgatc
     1501 agcatcgcca ccggccaggg gcgccaggcg ggcagcgctt ggcgcgggcg gttcgcccag
     1561 cgccgggcca gcgcccagaa acaggcggcg atccagcaga aggtgagcga ggtctccagg
     1621 cccgaggtgc cgaaatccca gaacggggcc agcccggcca gcgcggcggt gcccagcggc
     1681 agcaccacgc cggcgcgcgg gggcaccagg tgcatggcac ccaggccgag cagcagcatc
     1741 gccagtgtga tgaaacccgt acccagccac accatcacct cgcccacctc ggcccacggc
     1801 gcggcgagct tgccgagcgt cacgaagaac agccacagcg tggaggtgcc gacctcgacc
     1861 cgctcgttga ggttgtaggt gggcccgttg ccggctacca gctgcaggat cacccggacg
     1921 ttgatgaagc catcgtcgga gacccagcgg cgcagccagg tgagcacgcc gaccacgagc
     1981 gccggcagca ggatcgacag ggcgatccaa gccctgcggg cggaggacgc tgtggccacg
     2041 ggtttccttt cggtcggcgt ccatcatggc agccgtcggg aaagccgaag tgcagcggcg
     2101 ggaggcgcgg ccgaaatgag cgagtgaatc tgcctaccga actcggtaca ccaactcaca
     2161 cgttgcgggc gggcggcagt agggtcgtgc taatggacgt gctgctgctc ggaaccggcg
     2221 cctcggacgg ggtgccgaac tgttggtgtc gctgcccgac ctgtgccgag gcccgggcgt
     2281 ccggtgctat ccgcaccaat gcgtccgcac tggtcgacgg acggctgctg atcgactgcg
     2341 gccccgaggc gccccggcag gcccagcgtg ccggacgcga cctgaccggc gtccggtcgg
     2401 tgctgatctc gcacgcgcac gacgaccatc tggacccgtc gttcctgatg catcgcgggt
     2461 gggtgagcga gcagccgctg cgggtgatcg gccccgcgcc ggcgatcgag cggtgccgcg
     2521 actggctggc gcccgaccag agtgtggtga gcctcacggt ggtcaccgcg ggcgactcgc
     2581 tcgaa
//
LOCUS       DAOSUG010000463         2578 bp    DNA     linear   ENV 10-JUN-2024
DEFINITION  MAG TPA_asm: Micropruina sp. isolate AMR_MDS_2195
            NODE_16229_length_2578_cov_3.116924, whole genome shotgun sequence.
ACCESSION   DAOSUG010000463 DAOSUG010000000
VERSION     DAOSUG010000463.1
DBLINK      BioProject: PRJNA925477
            BioSample: SAMN32864262
            Sequence Read Archive: SRR4236673
KEYWORDS    WGS; ENV; Metagenome Assembled Genome; MAG; Third Party Data; TPA;
            TPA:assembly.
SOURCE      Micropruina sp. (wastewater metagenome)
  ORGANISM  Micropruina sp.
            Bacteria; Bacillati; Actinomycetota; Actinomycetes;
            Propionibacteriales; Nocardioidaceae; Micropruina.
REFERENCE   1  (bases 1 to 2578)
  AUTHORS   Abdulkadir,N., Saraiva,J.P., Zhang,J., Stolte,S., Gillor,O.,
            Harms,H. and Rocha,U.
  TITLE     Genome-centric analyses of 165 metagenomes show that mobile genetic
            elements are crucial for the transmission of antimicrobial
            resistance genes to pathogens in activated sludge and wastewater
  JOURNAL   Microbiol Spectr 12 (3), e0291823 (2024)
   PUBMED   38289113
REFERENCE   2  (bases 1 to 2578)
  AUTHORS   Abdulkadir,N.
  TITLE     Direct Submission
  JOURNAL   Submitted (24-MAR-2023) Environmental Microbiology, Helmholtz
            Center for Environmental Research -UFZ, Permoserstr 15, Leipzig
            04318, Germany
COMMENT     The annotation was added by the NCBI Prokaryotic Genome Annotation
            Pipeline (PGAP). Information about PGAP can be found here:
            https://www.ncbi.nlm.nih.gov/genome/annotation_prok/
            
            ##Genome-Assembly-Data-START##
            Assembly Method        :: SPAdes v. 3.11.1
            Genome Representation  :: Full
            Expected Final Version :: Yes
            Genome Coverage        :: Not Applicable
            Sequencing Technology  :: Illumina MiSeq
            ##Genome-Assembly-Data-END##
            
            ##Genome-Annotation-Data-START##
            Annotation Provider               :: NCBI
            Annotation Date                   :: 05/04/2023 04:57:10
            Annotation Pipeline               :: NCBI Prokaryotic Genome
                                                 Annotation Pipeline (PGAP)
            Annotation Method                 :: Best-placed reference protein
                                                 set; GeneMarkS-2+
            Annotation Software revision      :: 6.5
            Features Annotated                :: Gene; CDS; rRNA; tRNA; ncRNA
            Genes (total)                     :: 2,776
            CDSs (total)                      :: 2,750
            Genes (coding)                    :: 2,714
            CDSs (with protein)               :: 2,714
            Genes (RNA)                       :: 26
            tRNAs                             :: 24
            ncRNAs                            :: 2
            Pseudo Genes (total)              :: 36
            CDSs (without protein)            :: 36
            Pseudo Genes (ambiguous residues) :: 0 of 36
            Pseudo Genes (frameshifted)       :: 2 of 36
            Pseudo Genes (incomplete)         :: 32 of 36
            Pseudo Genes (internal stop)      :: 2 of 36
            ##Genome-Annotation-Data-END##
FEATURES             Location/Qualifiers
     source          1..2578
                     /organism="Micropruina sp."
                     /mol_type="genomic DNA"
                     /submitter_seqid="NODE_16229_length_2578_cov_3.116924"
                     /isolate="AMR_MDS_2195"
                     /isolation_source="Activated sludge of municipal
                     wastewater treatment plants"
                     /db_xref="taxon:2737536"
                     /environmental_sample
                     /geo_loc_name="Canada"
                     /lat_lon="49.25 N 123.1 W"
                     /collection_date="2015-06-16"
                     /metagenome_source="wastewater metagenome"
                     /note="metagenomic"
     gene            <1..1276
                     /locus_tag="PLF56_12745"
     CDS             <1..1276
                     /locus_tag="PLF56_12745"
                     /inference="COORDINATES: protein motif:HMM:NF022113.2"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=2
                     /transl_table=11
                     /product="LpqB family beta-propeller domain-containing
                     protein"
                     /protein_id="HOQ54467.1"
                     /translation="ADNQWRISQPPRGLLISQSLFSSTWVRADICLWDTTGTVLVPDP
                     RFVQKGTLGMTASVRALLQGPSATSEAAYRSPLVARLDVAAVSLNVNGTADVDLTGDV
                     DRLNAEARRRLAAELVWSLTSLEGVTGVRVRGNGTLWDLGGSSGIVTTADFSQAAPAL
                     PSSSDQVFVVRGGVLSRLGWNTPVAEAQVVAPSVTRLGPISARADGQEVAAVSDAGTK
                     VRLIAVREGTARVLLSGQGLTSVRFTRQGELLVVTDGVRGDRLRLVRDAHQAPIDAAG
                     LPTGRIRSLAVAPDGVRVALVIEEQGRSVLGVAAIVRTSEGMRFVGWRELSGAAWSPT
                     SQPPVDVGWSDSASLLVLLGGTAGPRVVSVDSEGAMATDVGPTDSVASAQLAVGGDSR
                     AVLRGSDGQTWRFIDEFTWDPWLSKVQQVSLP"
     gene            1326..2006
                     /locus_tag="PLF56_12750"
     CDS             1326..2006
                     /locus_tag="PLF56_12750"
                     /inference="COORDINATES: protein motif:HMM:NF012383.2"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_process: GO:0009116 - nucleoside metabolic process
                     [Evidence IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="phosphoribosyltransferase family protein"
                     /protein_id="HOQ54468.1"
                     /translation="MGWRAVMAAGADLAWGGACAACGRPGPVLCPDCARTLHELLPFE
                     VPGRAGVPVTVARGDYADDVRRVILACKERQGLTLVPVLAELAVGSAALLLERRWDGR
                     PLHLVPVPSARATVAARGFDLTAMMAARVARTLRRHRLDALVWPALRQLRPGRDQAGL
                     DVAQRAANRRASMSVRARAPRTVLLVDDIITTGATLSEASRALQQAGHTVLGAAVVAA
                     TPRTGAHT"
     gene            complement(2089..2427)
                     /locus_tag="PLF56_12755"
     CDS             complement(2089..2427)
                     /locus_tag="PLF56_12755"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_015583331.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="P-II family nitrogen regulator"
                     /protein_id="HOQ54469.1"
                     /translation="MKLVTAIIQPEVLEQVMKAVVRHGVGGMTISEVSGYGRQRGHVE
                     VYRGEEYTIDFITKVRLEIIVDDPECEEVVQVIAGSARTGNVGDGKVWVVPVENVVRV
                     RTGESGLAAV"
BASE COUNT          315 a          894 c          968 g          401 t
ORIGIN      
        1 cgccgacaac caatggcgca tctcgcagcc gccgcgcggc ctgctgatct cgcaatcgct
       61 gttcagcagc acctgggtgc gcgccgacat ctgcttgtgg gacaccaccg gaaccgtgct
      121 ggtgcccgac ccgcggttcg tccagaaggg cacgctcggc atgacggcat cggtgcgcgc
      181 cctcctgcag ggaccctccg cgacctcgga ggccgcctac cggtccccgc tcgtcgcgcg
      241 gctcgacgtc gccgccgtgt cgttgaacgt gaacggcacc gccgacgtcg atctgaccgg
      301 cgacgtcgac cggctgaacg ccgaggcgcg gcgccggttg gccgccgaac tggtctggtc
      361 gctgacctcg ctcgagggcg tgaccggtgt gcgggtgcgg ggcaacggca cactgtggga
      421 cctgggcggc agctcgggga tcgtgaccac cgcggacttc agccaggccg cgccggccct
      481 gccgagctcg tccgatcagg tgttcgtggt gcgcggcggc gtgctgtcac ggctcggctg
      541 gaacaccccc gtggccgagg cgcaggtggt ggcgccgtcc gtgacccggc tcggcccgat
      601 ctcggcccgt gccgacgggc aggaggtggc cgcggtcagc gacgcaggca ccaaggtgcg
      661 cctgatcgcc gtccgcgagg gcacggcgcg ggtcctactg agcgggcagg gcctgacctc
      721 ggtgcgcttc acccggcagg gcgaactgct ggtggtcacc gatggggtgc gcggcgaccg
      781 gctgcggctg gtgcgtgacg cgcaccaggc cccgatcgac gcggccgggc taccgaccgg
      841 gcggatcaga tcgctcgccg tcgccccgga cggggtgcgg gtggcactcg tgatcgaaga
      901 gcagggccgg tcggtgctgg gcgtcgcggc catcgtccgg acgtccgagg gcatgcggtt
      961 cgtcggctgg cgtgagttgt ccggcgccgc gtggtcaccc accagccagc cgcccgtcga
     1021 cgtcggctgg tccgattcgg cgagcctgtt ggtgctgctg gggggcaccg ccggcccccg
     1081 cgtggtgagt gtcgacagcg agggtgcgat ggccaccgac gtgggcccga ccgactcggt
     1141 cgcctcggcg cagctggcgg tgggcggcga ctcgagggcg gtgctgcgcg gctccgacgg
     1201 gcagacctgg cgcttcatcg acgagttcac ctgggacccg tggttgtcga aagtccagca
     1261 ggtttccctg ccctgagcct gtggagggcg acccaccagc gtccggtggc gggcatcatc
     1321 gctgcatggg gtggcgcgcg gtgatggctg ccggagccga cctggcgtgg ggcggcgcgt
     1381 gcgccgcctg cggccggccc ggcccggtgc tgtgccccga ctgcgcccgg acgctgcacg
     1441 agctcttgcc gttcgaggtg ccgggccgag ccggagtgcc ggtcaccgtc gcacggggcg
     1501 attacgccga tgacgtgcgc cgggtgattc tggcctgcaa ggaacgtcag ggactcacgc
     1561 tggtgcccgt gctggctgag ttggcggtgg gcagcgccgc gttgctgctc gaacggcgtt
     1621 gggacggccg tcccttgcat ctggtgccgg tgccgtcggc ccgggcgacg gtcgcggcgc
     1681 gcggcttcga cctgacggcg atgatggcgg cgcgggtggc tcgcacgttg cggcgtcatc
     1741 ggctggacgc cctggtctgg ccggcgttgc ggcagctgcg ccccgggcgc gatcaggcgg
     1801 gcctggacgt ggcgcaacgc gccgcgaacc ggcgggccag catgagcgtg cgggcccgag
     1861 cgccgcgcac ggtgctgctg gtggacgaca tcatcaccac gggcgccacc ttgagcgagg
     1921 cgtcccgcgc attgcagcag gcgggccaca ccgtgctggg tgcggccgtg gtggcagcga
     1981 ctccgcgcac cggcgcacac acgtgagggg ccccgagcga actcggagcc cctcacggtt
     2041 accgccgcga cctgcccgcc cccacgtagc ggtcgcggag cctgcgggtc agacggcggc
     2101 caggccgctc tcgccggtgc gcacccggac gacgttctcg acgggcacca cccacacctt
     2161 gccgtcaccg acgttgccgg tacgggccga gccggcgatc acctgcacca cctcttcgca
     2221 ctcggggtcg tccacgatga tctcgagccg caccttggtg atgaagtcga tcgtgtactc
     2281 ctcgccgcgg tagacctcga cgtgtccgcg ctggcgaccg tagccggaca cctcgctgat
     2341 cgtcatgccg ccgacgccgt gacggaccac ggccttcatg acctgctcca gcacttcggg
     2401 ctgaatgatc gcggttacca gcttcacttg cctgctcctg tcgtttcggt ctcgggcagg
     2461 tcgtccttgg tcaggaagag cgtgcgctgg accttgttgg tgtagtagac cgggctgagg
     2521 tcgtacccgg cctcggaatg ttcgaccaga tcgatgccgc cgatctcgtc ggaggtgc
//
LOCUS       DAOSUG010000464         2572 bp    DNA     linear   ENV 10-JUN-2024
DEFINITION  MAG TPA_asm: Micropruina sp. isolate AMR_MDS_2195
            NODE_16281_length_2572_cov_3.553834, whole genome shotgun sequence.
ACCESSION   DAOSUG010000464 DAOSUG010000000
VERSION     DAOSUG010000464.1
DBLINK      BioProject: PRJNA925477
            BioSample: SAMN32864262
            Sequence Read Archive: SRR4236673
KEYWORDS    WGS; ENV; Metagenome Assembled Genome; MAG; Third Party Data; TPA;
            TPA:assembly.
SOURCE      Micropruina sp. (wastewater metagenome)
  ORGANISM  Micropruina sp.
            Bacteria; Bacillati; Actinomycetota; Actinomycetes;
            Propionibacteriales; Nocardioidaceae; Micropruina.
REFERENCE   1  (bases 1 to 2572)
  AUTHORS   Abdulkadir,N., Saraiva,J.P., Zhang,J., Stolte,S., Gillor,O.,
            Harms,H. and Rocha,U.
  TITLE     Genome-centric analyses of 165 metagenomes show that mobile genetic
            elements are crucial for the transmission of antimicrobial
            resistance genes to pathogens in activated sludge and wastewater
  JOURNAL   Microbiol Spectr 12 (3), e0291823 (2024)
   PUBMED   38289113
REFERENCE   2  (bases 1 to 2572)
  AUTHORS   Abdulkadir,N.
  TITLE     Direct Submission
  JOURNAL   Submitted (24-MAR-2023) Environmental Microbiology, Helmholtz
            Center for Environmental Research -UFZ, Permoserstr 15, Leipzig
            04318, Germany
COMMENT     The annotation was added by the NCBI Prokaryotic Genome Annotation
            Pipeline (PGAP). Information about PGAP can be found here:
            https://www.ncbi.nlm.nih.gov/genome/annotation_prok/
            
            ##Genome-Assembly-Data-START##
            Assembly Method        :: SPAdes v. 3.11.1
            Genome Representation  :: Full
            Expected Final Version :: Yes
            Genome Coverage        :: Not Applicable
            Sequencing Technology  :: Illumina MiSeq
            ##Genome-Assembly-Data-END##
            
            ##Genome-Annotation-Data-START##
            Annotation Provider               :: NCBI
            Annotation Date                   :: 05/04/2023 04:57:10
            Annotation Pipeline               :: NCBI Prokaryotic Genome
                                                 Annotation Pipeline (PGAP)
            Annotation Method                 :: Best-placed reference protein
                                                 set; GeneMarkS-2+
            Annotation Software revision      :: 6.5
            Features Annotated                :: Gene; CDS; rRNA; tRNA; ncRNA
            Genes (total)                     :: 2,776
            CDSs (total)                      :: 2,750
            Genes (coding)                    :: 2,714
            CDSs (with protein)               :: 2,714
            Genes (RNA)                       :: 26
            tRNAs                             :: 24
            ncRNAs                            :: 2
            Pseudo Genes (total)              :: 36
            CDSs (without protein)            :: 36
            Pseudo Genes (ambiguous residues) :: 0 of 36
            Pseudo Genes (frameshifted)       :: 2 of 36
            Pseudo Genes (incomplete)         :: 32 of 36
            Pseudo Genes (internal stop)      :: 2 of 36
            ##Genome-Annotation-Data-END##
FEATURES             Location/Qualifiers
     source          1..2572
                     /organism="Micropruina sp."
                     /mol_type="genomic DNA"
                     /submitter_seqid="NODE_16281_length_2572_cov_3.553834"
                     /isolate="AMR_MDS_2195"
                     /isolation_source="Activated sludge of municipal
                     wastewater treatment plants"
                     /db_xref="taxon:2737536"
                     /environmental_sample
                     /geo_loc_name="Canada"
                     /lat_lon="49.25 N 123.1 W"
                     /collection_date="2015-06-16"
                     /metagenome_source="wastewater metagenome"
                     /note="metagenomic"
     gene            271..1005
                     /locus_tag="PLF56_12760"
     CDS             271..1005
                     /locus_tag="PLF56_12760"
                     /inference="COORDINATES: ab initio
                     prediction:GeneMarkS-2+"
                     /note="Derived by automated computational analysis using
                     gene prediction method: GeneMarkS-2+."
                     /codon_start=1
                     /transl_table=11
                     /product="hypothetical protein"
                     /protein_id="HOQ54470.1"
                     /translation="MLLPRVVVGLAASLALLSGCSGAATTQPSAAASGTSATTATSAP
                     AGTALTPEAFVDKVATAMKSVTSYTTDMTITNGDKKSTMQAVVKADNGRTTAMRLTMT
                     GEAATEMIVIDGKYFMKSAQGWVKMPESTTKSMDLSQTDQTQWMRDNKDAITAVDLVG
                     SENVNGVQADHYRVTLDLSKASGASASTARISNYEAWLDSEGRMVKVLMTAEMGGTKT
                     VTEVLVSKYNEPVEIKAPADYTEMPG"
     gene            complement(1419..>2572)
                     /locus_tag="PLF56_12765"
     CDS             complement(1419..>2572)
                     /locus_tag="PLF56_12765"
                     /inference="COORDINATES: protein motif:HMM:NF014307.2"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=3
                     /transl_table=11
                     /product="NAD(P)(+) transhydrogenase (Re/Si-specific)
                     subunit beta"
                     /protein_id="HOQ54471.1"
                     /translation="VKMTDMPQMVAIYNGMGGGAAALIAAVEFARAEVHGPIVGTLAV
                     LGSLIGSVAFSGSVIAFLKLQGIMKKAIRLPGQNLINIALGLITIGLGVWIVISSVGV
                     GTANPVLLVMFFVLSLALGAILTMPIGGADMPVVISLLNAFTGLAVGFEGYVIGNPTL
                     IIAGIVVGASGALLTQLMAKAMNRPILGVLFTPISGEAQAGGAIEGSMKEMTATDAAA
                     LMRYSEKVIVIPGYGMAVAGAQHKVWEMAKLLEEQGVEVSFAIHPVAGRMPGHMNVLL
                     AEAGVPYEKIFDLDEINAEFPQTDVALVIGANDVVNPVARTDKSSPIYGMPILDADKA
                     RNCIVIKRGRGAGYSGVENALFYADNTSMLYGSAQQAVGDVIAQLKELV"
BASE COUNT          449 a          877 c          819 g          427 t
ORIGIN      
        1 tgaccctcgg cgtagcgggc attcgaatgc ggctcgcgcc ggctgagcgc cggcaggtcg
       61 tcctgtgtgc acagccgcac cgaaataggc tccgtcatgg gtacctccag cgtcgagggg
      121 tgctgcgatg gctcagtttg ccagtccacc gcaagcctat ggccgggcgc aactggtcgc
      181 agcccctggc gccggtgtct cgacaacccg ccccgacccg gtctcatggc atactccaga
      241 cgaccatttg ctctactcaa aggatgtttc atgctgctac cgcgcgtcgt cgtcggtctc
      301 gccgcctccc tcgccctact cagtggttgc tccggtgcag ccaccacgca gccgtccgct
      361 gcggcgagcg gcacgtctgc caccacggcc acctcggccc cggccggcac tgctttgacg
      421 ccggaggcgt tcgtcgacaa ggtggccacc gcgatgaagt cggtgaccag ctacaccacc
      481 gacatgacca tcaccaacgg tgacaagaag agcaccatgc aggccgtggt caaggccgac
      541 aacggtcgca ccaccgccat gcgcctgacg atgaccggcg aggcggccac cgaaatgatc
      601 gtgatcgacg gcaagtactt catgaagtcg gcgcaggggt gggtcaagat gcccgagagc
      661 accaccaagt cgatggacct gtcccagacc gatcagaccc agtggatgcg cgacaacaag
      721 gacgcgatca cggccgtcga cctggtcggc tccgagaacg tcaacggcgt gcaggccgat
      781 cactaccgcg tcacgctcga cctgtcgaag gcctcgggtg cgagtgcgtc caccgcccgc
      841 atctcgaact acgaggcctg gctcgactcc gagggtcgca tggtgaaggt gctgatgacc
      901 gccgagatgg gcggtaccaa gaccgtcacc gaggtcctgg tgtcgaagta caacgagccg
      961 gtcgagatca aggcccccgc ggactacacc gagatgcccg gctgagctga gtcgccatcg
     1021 cggttgcggt gggcgcatcc gccgcgaaac tgcaccacat ttgcacgcct gcttgcctcg
     1081 cgagcgccaa ggactgcgga atgtggtgca gtttcgcacg tcggcgtcgg ctcgaggtgt
     1141 ggggatgtgg cgcaggctag ctcgtgagcg tcagtctcga cgcgtatcga actgcaccac
     1201 attggtgggg ttgttcgcct actgaggcgg agacgctgcc gaatctggtg cagtttcgcg
     1261 cgcgagcggt cgtccggacg cacgcgaacg gtacgaccga cccggtttcg gcacgcccga
     1321 tctttcggac gacaggtgcg cccggccccg cacgtcactc gggctcgcgc ttctttcgga
     1381 cggccggggc ccggcctgcg tgtgaccccg cgctttgatc agaccagttc cttcaactgc
     1441 gcgatcacgt cgccgacggc ctgctgggcg ctgccgtaga gcatggacgt gttgtcggcg
     1501 tagaacagcg cgttctcgac accggagtag ccggcgcccc ggccacgctt gatcacgatg
     1561 cagttgcggg ccttgtcggc atcgaggatc ggcatgccgt agatcggcga gctcttgtcg
     1621 gtgcgggcca ccgggttcac cacgtcgttg gcgccgatca ccagtgccac gtcggtctgc
     1681 ggaaactcgg cgttgatctc gtccaggtcg aagatcttct cgtacggcac accggcctcg
     1741 gcgagcagca cgttcatgtg gcccggcatg cggcccgcga ccgggtgaat ggcgaacgac
     1801 acctcgactc cctgctcttc gagcagcttc gccatctccc acaccttgtg ctgcgccccc
     1861 gccacggcca tgccgtagcc gggtatcacg atcaccttct cggagtagcg catcagggcg
     1921 gccgcgtcgg tggcggtcat ctctttcatg ctgccctcga tggcgccgcc ggcctgcgcc
     1981 tcgccgctga tcggggtgaa cagcaccccc aggatcgggc ggttcatcgc cttggccatc
     2041 aactgggtca gcagggcgcc ggacgcgccc accacgatgc ccgcgatgat cagcgtcggg
     2101 ttgccgatca cgtagccctc gaagccgacc gcgaggcccg tgaaggcgtt gagcagcgag
     2161 atcaccaccg gcatgtcggc gccgccgatc ggcatggtca ggatcgcgcc gagggccaat
     2221 gacaacacga agaacatcac cagaagcacc gggttcgcgg tgcccacccc gaccgacgaa
     2281 atgacgatcc acacgccgag gccgatggta atcaggccca gcgcgatgtt gatcaggttc
     2341 tgcccgggca gccggatggc cttcttcatg atgccctgca gcttcaggaa ggcgatcacc
     2401 gagccgctga aggcaaccga accgatcagc gatccgagca ccgccagggt gccgacgatc
     2461 gggccgtgca cctcggctcg ggcgaactca acggccgcga tcagtgccgc cgcaccaccg
     2521 cccatgccgt tgtagatggc gaccatctgc ggcatgtcgg tcatcttgac ca
//
LOCUS       DAOSUG010000465         2570 bp    DNA     linear   ENV 10-JUN-2024
DEFINITION  MAG TPA_asm: Micropruina sp. isolate AMR_MDS_2195
            NODE_16300_length_2570_cov_3.796024, whole genome shotgun sequence.
ACCESSION   DAOSUG010000465 DAOSUG010000000
VERSION     DAOSUG010000465.1
DBLINK      BioProject: PRJNA925477
            BioSample: SAMN32864262
            Sequence Read Archive: SRR4236673
KEYWORDS    WGS; ENV; Metagenome Assembled Genome; MAG; Third Party Data; TPA;
            TPA:assembly.
SOURCE      Micropruina sp. (wastewater metagenome)
  ORGANISM  Micropruina sp.
            Bacteria; Bacillati; Actinomycetota; Actinomycetes;
            Propionibacteriales; Nocardioidaceae; Micropruina.
REFERENCE   1  (bases 1 to 2570)
  AUTHORS   Abdulkadir,N., Saraiva,J.P., Zhang,J., Stolte,S., Gillor,O.,
            Harms,H. and Rocha,U.
  TITLE     Genome-centric analyses of 165 metagenomes show that mobile genetic
            elements are crucial for the transmission of antimicrobial
            resistance genes to pathogens in activated sludge and wastewater
  JOURNAL   Microbiol Spectr 12 (3), e0291823 (2024)
   PUBMED   38289113
REFERENCE   2  (bases 1 to 2570)
  AUTHORS   Abdulkadir,N.
  TITLE     Direct Submission
  JOURNAL   Submitted (24-MAR-2023) Environmental Microbiology, Helmholtz
            Center for Environmental Research -UFZ, Permoserstr 15, Leipzig
            04318, Germany
COMMENT     The annotation was added by the NCBI Prokaryotic Genome Annotation
            Pipeline (PGAP). Information about PGAP can be found here:
            https://www.ncbi.nlm.nih.gov/genome/annotation_prok/
            
            ##Genome-Assembly-Data-START##
            Assembly Method        :: SPAdes v. 3.11.1
            Genome Representation  :: Full
            Expected Final Version :: Yes
            Genome Coverage        :: Not Applicable
            Sequencing Technology  :: Illumina MiSeq
            ##Genome-Assembly-Data-END##
            
            ##Genome-Annotation-Data-START##
            Annotation Provider               :: NCBI
            Annotation Date                   :: 05/04/2023 04:57:10
            Annotation Pipeline               :: NCBI Prokaryotic Genome
                                                 Annotation Pipeline (PGAP)
            Annotation Method                 :: Best-placed reference protein
                                                 set; GeneMarkS-2+
            Annotation Software revision      :: 6.5
            Features Annotated                :: Gene; CDS; rRNA; tRNA; ncRNA
            Genes (total)                     :: 2,776
            CDSs (total)                      :: 2,750
            Genes (coding)                    :: 2,714
            CDSs (with protein)               :: 2,714
            Genes (RNA)                       :: 26
            tRNAs                             :: 24
            ncRNAs                            :: 2
            Pseudo Genes (total)              :: 36
            CDSs (without protein)            :: 36
            Pseudo Genes (ambiguous residues) :: 0 of 36
            Pseudo Genes (frameshifted)       :: 2 of 36
            Pseudo Genes (incomplete)         :: 32 of 36
            Pseudo Genes (internal stop)      :: 2 of 36
            ##Genome-Annotation-Data-END##
FEATURES             Location/Qualifiers
     source          1..2570
                     /organism="Micropruina sp."
                     /mol_type="genomic DNA"
                     /submitter_seqid="NODE_16300_length_2570_cov_3.796024"
                     /isolate="AMR_MDS_2195"
                     /isolation_source="Activated sludge of municipal
                     wastewater treatment plants"
                     /db_xref="taxon:2737536"
                     /environmental_sample
                     /geo_loc_name="Canada"
                     /lat_lon="49.25 N 123.1 W"
                     /collection_date="2015-06-16"
                     /metagenome_source="wastewater metagenome"
                     /note="metagenomic"
     gene            complement(126..>2570)
                     /locus_tag="PLF56_12770"
     CDS             complement(126..>2570)
                     /locus_tag="PLF56_12770"
                     /inference="COORDINATES: protein motif:HMM:NF013239.2"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_function: GO:0004553 - hydrolase activity, hydrolyzing
                     O-glycosyl compounds [Evidence IEA];
                     GO_process: GO:0005975 - carbohydrate metabolic process
                     [Evidence IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="glycoside hydrolase family 31 protein"
                     /protein_id="HOQ54472.1"
                     /translation="AVNPGDEVPLDKSKLQNLKVTKVTATSDGVKLTMGSFVGLVRFV
                     SADVVKVTLQAAGQPAYQSVAVVKNIISKVSIKRTKTSSTYTMKTKSVTVRIALGKFG
                     VTMLDKSGRVINADDSRFGSGYENGKPYVFKKTDKDEAFYGFGEQTKGLNKRGDSIGM
                     WNTDHYAYEADAKYIYTSIPFFTGLKSSGAYGIFFDNTHHSMYEMASESPDYYYFYAN
                     GGELNYYFINGPKMADVLDRYTQLTGRYQQPPEWSLGWEQSHWGYFPGSKIVDVAKGY
                     RERKIPLDAMNMDIDYMDGWRVFTWGKNWGDQNKIDDQLESMGVKTIAINDPGVKKES
                     GYWMSDEGTAKGYWATNPDGSNYEGAVWPGTAHFPDFSRADVRTWWADQMPKLTDKGV
                     DGIWLDMNEPADFVGPNHTLPLNVEFSNGTKQHTEVHNIYGFWNTVATYEGLKKVKPN
                     ERAFVFSRDMYAGSQRYAALWSGDNVSSWDHLALTMPLNMNVGLSGVPHVGNDIGGFV
                     GDTTPELFARWIESGSFAPFARVHYDNNFQPDQQCQEPWCLGSEVEDIARKYVSLRYS
                     LLPYTNTAFHNATKDGRPVQQALVYQFQNDPAVRNISDQFMWGDSIMVAPVVTKGATS
                     RQVYLPKGKDWIDWWTGEKHTGGQTITKDAPLDVMPIYVAAGSVISTREVQQHTGQND
                     FTNVAFNVFLGNGKATGTLYEDDGRTEAYKKGAYNTYRVDVSSAANGSVSISTKKTHS
                     GYSSKLKTYDVVLRGDLPVFGGATGATVKPASVALNSEASTRGVKIAKVDAKTGTVTV
                     RVPISAGNVTIAFEGK"
BASE COUNT          397 a          794 c          824 g          555 t
ORIGIN      
        1 ggccgacagc gaggcgacgg tcaggtcgtt cggtgcggcc acggcccgcg gtggcaggga
       61 tgagaggacc agcgccccgg ccacggcgac cgccgcagtg agcttcagtg gctgcttcat
      121 catgatcact tcccctcgaa cgcgatggtg acgttgccgg ccgagatcgg cacacgaacg
      181 gtcaccgtgc cggtcttggc gtccaccttg gcgatcttca caccacgggt ggacgcctcg
      241 gagttgagcg cgaccgaggc cggcttcacc gtggcgcccg tggcaccgcc gaacaccggc
      301 aggtcgccgc gcagcaccac gtcgtaggtc ttgagcttgg acgaatatcc cgaatgggtc
      361 ttcttggtcg agatcgagac cgagccgttc gcagcggacg agacgtcgac gcggtaggtg
      421 ttgtaggcgc ccttcttgta cgcctcggtc ctgccgtcgt cctcatacag ggtgccggtg
      481 gccttgccgt tgccgaggaa cacgttgaac gccacgttgg tgaagtcgtt ctgcccggtg
      541 tgctgctgca cctcgcgggt cgagatcacc gatccggcgg ccacgtagat cggcatcacg
      601 tcgagcggcg cgtccttggt gatggtctga ccgccggtgt gcttctcacc ggtccaccag
      661 tcgatccagt ccttgccctt gggcagatag acctgacgcg aggtggcgcc cttggtgacc
      721 accggcgcga ccatgatcga gtcgccccac atgaactgat ccgagatgtt gcgcaccgcc
      781 ggatcgttct ggaactggta gaccagcgcc tgctgcaccg ggcgtccgtc cttggtggcg
      841 ttgtggaaag ccgtgttggt gtacggcagc aggctgtagc gcaggctgac atacttgcgg
      901 gcgatgtcct cgacctccga acccaggcac cagggctcct ggcactgctg gtcgggctgg
      961 aagttgttgt cgtagtgcac gcgggcgaac ggcgcgaacg agccgctttc gatccagcgg
     1021 gcgaacagct ccggggtggt gtcgccgacg aagccgccga tgtcgttgcc gacgtgcggc
     1081 acccccgaca gtcccacgtt catgttgagc ggcatggtca gggcgaggtg gtcccaactg
     1141 ctgacgttgt cgccgctcca caacgccgcg taacgctgag aaccagcgta catgtcacgg
     1201 ctgaacacga aggcgcgctc gttcggcttg accttcttga gcccctcgta ggtggccacg
     1261 gtgttccaaa agccgtagat gttgtggacc tcggtgtgtt gcttggtgcc gttgctgaac
     1321 tccacgttca gcggcaacgt gtggttgggt ccgacgaagt cggccggctc gttcatgtcg
     1381 agccagatgc cgtccacgcc cttgtcggtt agcttgggca tctggtccgc ccaccatgtg
     1441 cggacgtcgg cccggctgaa gtcggggaag tgggcggtac cgggccacac agcgccctcg
     1501 tagttgctgc cgtcggggtt ggtcgcccag tagcccttgg ccgtgccctc gtcggacatc
     1561 cagtagccgc tctctttctt gacgcctggg tcgttgatgg cgatcgtctt cacccccatc
     1621 gactccagct gatcgtcgat cttgttctgg tcaccccagt tcttgcccca ggtgaacact
     1681 cgccagccat ccatgtagtc gatgtccatg ttcatcgcgt ccagcgggat cttccgttcg
     1741 cggtagccct tggcgacgtc gacgatcttg ctgcccggga agtatcccca gtggctttgc
     1801 tcccacccca gtgaccattc cggaggctgc tggtagcgcc cggtcaactg ggtgtatcgg
     1861 tcgagcacgt cggccatctt tggcccgttg atgaagtagt agttcagttc tccgccgttg
     1921 gcgtagaaat agtagtagtc gggcgattcg ctggccatct cgtacatcga gtggtgggtg
     1981 ttgtcgaaga agatgccgta ggcgccggag ctcttcaagc ccgtgaagaa gggaatcgag
     2041 gtgtagatgt acttagcgtc ggcctcatag gcgtagtggt cggtgttcca cattccgatg
     2101 ctgtcgccgc gcttgttcag acccttggtc tgctcgccga aaccgtagaa tgcctcgtcc
     2161 ttgtcggtct tcttgaacac gtacggcttg ccgttctcgt agccgctgcc gaaccgagag
     2221 tcgtcggcgt tgatcaccct gcccgacttg tcgagcatgg tgacgccgaa cttgcccagc
     2281 gcgatgcgca ccgtcacgct cttggtcttc atcgtgtacg tggacgaggt cttggtgcgc
     2341 ttgatcgata ccttgctgat gatgttcttc accaccgcca cgctctggta cgcgggttgg
     2401 ccggcggcct gcagggtgac cttcaccacg tctgcgctca cgaagcgcac cagccccacg
     2461 aaggagccca tggtgagctt caccccgtcg ctggtggcgg tgaccttggt taccttcagg
     2521 ttctgaagtt tggatttgtc gagcggcact tcatcgccgg ggttgacggc
//
LOCUS       DAOSUG010000466         2567 bp    DNA     linear   ENV 10-JUN-2024
DEFINITION  MAG TPA_asm: Micropruina sp. isolate AMR_MDS_2195
            NODE_16328_length_2567_cov_4.963376, whole genome shotgun sequence.
ACCESSION   DAOSUG010000466 DAOSUG010000000
VERSION     DAOSUG010000466.1
DBLINK      BioProject: PRJNA925477
            BioSample: SAMN32864262
            Sequence Read Archive: SRR4236673
KEYWORDS    WGS; ENV; Metagenome Assembled Genome; MAG; Third Party Data; TPA;
            TPA:assembly.
SOURCE      Micropruina sp. (wastewater metagenome)
  ORGANISM  Micropruina sp.
            Bacteria; Bacillati; Actinomycetota; Actinomycetes;
            Propionibacteriales; Nocardioidaceae; Micropruina.
REFERENCE   1  (bases 1 to 2567)
  AUTHORS   Abdulkadir,N., Saraiva,J.P., Zhang,J., Stolte,S., Gillor,O.,
            Harms,H. and Rocha,U.
  TITLE     Genome-centric analyses of 165 metagenomes show that mobile genetic
            elements are crucial for the transmission of antimicrobial
            resistance genes to pathogens in activated sludge and wastewater
  JOURNAL   Microbiol Spectr 12 (3), e0291823 (2024)
   PUBMED   38289113
REFERENCE   2  (bases 1 to 2567)
  AUTHORS   Abdulkadir,N.
  TITLE     Direct Submission
  JOURNAL   Submitted (24-MAR-2023) Environmental Microbiology, Helmholtz
            Center for Environmental Research -UFZ, Permoserstr 15, Leipzig
            04318, Germany
COMMENT     The annotation was added by the NCBI Prokaryotic Genome Annotation
            Pipeline (PGAP). Information about PGAP can be found here:
            https://www.ncbi.nlm.nih.gov/genome/annotation_prok/
            
            ##Genome-Assembly-Data-START##
            Assembly Method        :: SPAdes v. 3.11.1
            Genome Representation  :: Full
            Expected Final Version :: Yes
            Genome Coverage        :: Not Applicable
            Sequencing Technology  :: Illumina MiSeq
            ##Genome-Assembly-Data-END##
            
            ##Genome-Annotation-Data-START##
            Annotation Provider               :: NCBI
            Annotation Date                   :: 05/04/2023 04:57:10
            Annotation Pipeline               :: NCBI Prokaryotic Genome
                                                 Annotation Pipeline (PGAP)
            Annotation Method                 :: Best-placed reference protein
                                                 set; GeneMarkS-2+
            Annotation Software revision      :: 6.5
            Features Annotated                :: Gene; CDS; rRNA; tRNA; ncRNA
            Genes (total)                     :: 2,776
            CDSs (total)                      :: 2,750
            Genes (coding)                    :: 2,714
            CDSs (with protein)               :: 2,714
            Genes (RNA)                       :: 26
            tRNAs                             :: 24
            ncRNAs                            :: 2
            Pseudo Genes (total)              :: 36
            CDSs (without protein)            :: 36
            Pseudo Genes (ambiguous residues) :: 0 of 36
            Pseudo Genes (frameshifted)       :: 2 of 36
            Pseudo Genes (incomplete)         :: 32 of 36
            Pseudo Genes (internal stop)      :: 2 of 36
            ##Genome-Annotation-Data-END##
FEATURES             Location/Qualifiers
     source          1..2567
                     /organism="Micropruina sp."
                     /mol_type="genomic DNA"
                     /submitter_seqid="NODE_16328_length_2567_cov_4.963376"
                     /isolate="AMR_MDS_2195"
                     /isolation_source="Activated sludge of municipal
                     wastewater treatment plants"
                     /db_xref="taxon:2737536"
                     /environmental_sample
                     /geo_loc_name="Canada"
                     /lat_lon="49.25 N 123.1 W"
                     /collection_date="2015-06-16"
                     /metagenome_source="wastewater metagenome"
                     /note="metagenomic"
     gene            complement(<1..893)
                     /locus_tag="PLF56_12775"
     CDS             complement(<1..893)
                     /locus_tag="PLF56_12775"
                     /inference="COORDINATES: ab initio
                     prediction:GeneMarkS-2+"
                     /note="Derived by automated computational analysis using
                     gene prediction method: GeneMarkS-2+."
                     /codon_start=1
                     /transl_table=11
                     /product="hypothetical protein"
                     /protein_id="HOQ54473.1"
                     /translation="MTRPQTAAGARLLAGLVLLCAVLAGVSAPRAFAAESVATYAVEG
                     SIGTDGALVVNATITFDGAAPATLVQSFATTREVMGDREYLFTLHDIKVTAGGQPLNA
                     TIAQQADRTVVTMPTQGVTSPVVLSYVVTGAAIKEASGETTVNWRLLQGLSLPVTQFT
                     ATLTVPTAISLVQCNAGPPVAPGVCTFWGGGTHDYPDPTFNDGPRGAGEVVEVIVRFP
                     AGTVSANEQIREQWTFERAFSVEPLPLTLALAALALGALGFWLLHRRIGRDAVGGEPT
                     PVAEFHPVGDGESEFRVLDGVR"
     gene            1041..2198
                     /locus_tag="PLF56_12780"
     CDS             1041..2198
                     /locus_tag="PLF56_12780"
                     /inference="COORDINATES: protein motif:HMM:NF016143.2"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="neutral zinc metallopeptidase"
                     /protein_id="HOQ54474.1"
                     /translation="MTQQYPPQQGYPQQWGQSQGWPAAPSHQGWNRPQQSFGQTAWPT
                     QQAGGFQPQAPYGYPPQPGGYHQPGQFTPQPPRRRNPLGRVLMLLVAVCGLLLAGVVV
                     ANLMSGGAQGTTPDPQQTWTPPPPDMSPPPLPTPKTYSEATEWMQNNPFYRQSIAIPT
                     NCSQLKPIDALTASESELNTHLQNLTSCLLQVWKAPVEAAGFEMPRPPATVYNQPIQT
                     ACGKLDSVNASYCGADQRIYYAKPLPKIFPRDLQGQKFLMEMVLGHEFGHAIQARTGI
                     SISSSAWEQRASTKAAANVFSRRLEVQADCFAGMWIHAVGESQGLTSSDLDGLRIIAY
                     NLGDDVLSGRQNIDSGHGLGKTRQRWFETGLTGSPQTLQCNTYSVPDAQVR"
     gene            complement(2273..>2567)
                     /locus_tag="PLF56_12785"
     CDS             complement(2273..>2567)
                     /locus_tag="PLF56_12785"
                     /inference="COORDINATES: protein motif:HMM:NF025054.2"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_function: GO:0008080 - N-acetyltransferase activity
                     [Evidence IEA]"
                     /codon_start=2
                     /transl_table=11
                     /product="GNAT family N-acetyltransferase"
                     /protein_id="HOQ54475.1"
                     /translation="RLPGLTLLIAEIDGEGVGTALSVVTPRVNNIGSLAVMPARRGRG
                     IGSALTMAAVAVAPHMPATLSATPSGRGVYQRLGFSEVGRPLHHHPMAEPGTD"
BASE COUNT          438 a          904 c          860 g          365 t
ORIGIN      
        1 cggacgccgt ccagcacccg gaactcggac tcaccgtcgc ccaccgggtg aaactcggcc
       61 accggcgtcg gctcgccgcc gacggcgtca cgtccgatgc gccggtgcag cagccagaac
      121 ccgagcgcac ccagcgcaag ggcggccagt gccagggtca acgggagcgg ctcgaccgag
      181 aaggcgcgtt cgaaggtcca ctgttcgcgg atctgttcgt tcgcgctgac cgttcccgcc
      241 gggaaccgga cgatcacctc gaccacctcg cccgcgccgc gcggaccgtc gttgaaggtg
      301 ggatcgggat agtcatgggt gccgccgccc cagaacgtgc agacccccgg cgccaccggg
      361 ggcccggcgt tgcattgcac caacgagatc gcggtgggaa cggtgagggt ggcggtgaac
      421 tgcgtcaccg gcaggctgag cccctgcagc agccgccagt tgaccgtcgt ttcgccgctc
      481 gcctctttga tcgccgcacc tgtcaccaca tagctcagga cgaccggcga cgtcacgccc
      541 tgggtgggca tggtcaccac cgtccgatcg gcctgctgcg cgatcgtggc attcagtggc
      601 tgtccgccgg cggtcacctt gatgtcgtgc agcgtaaaca ggtactcgcg atcacccatc
      661 acctcacggg tggtggcgaa gctctgcacc agtgtcgccg gcgcggcccc gtcgaaggtg
      721 atcgtggcgt tgaccacgag cgccccgtcg gtgccgatcg atccctccac cgcgtaggtg
      781 gcgacggact cggccgcgaa ggcccgtggt gccgacaccc cggccagcac ggcgcacagc
      841 agcaccagtc cagccagcag tcgggcacct gcagcggttt gcggtctcgt cacgatcgtc
      901 ccttcggaaa acaaggcaac acgggcgcgg ggcgcagcac gaagctatcg caaaccgagc
      961 ccgcgcgacc cgcatcggtg cgcaggaatt ggtggcgccg acaagggtcg gcgcggctcg
     1021 cctgccgtta cagtgttcgc gtgacgcagc agtatccgcc tcagcagggc tacccccagc
     1081 agtggggcca gtcccagggc tggcctgcgg ccccttcgca tcagggttgg aaccggcctc
     1141 agcagtcgtt cggccaaacc gcgtggccga cccagcaggc cggcggtttt caaccgcagg
     1201 cgccatacgg ctacccaccg caaccgggcg gctatcacca gcccggacag ttcacccctc
     1261 agccaccccg gcggcgtaac ccgctggggc gggtgttgat gctgctggta gcggtgtgtg
     1321 gcctgctgct ggccggtgtg gtggtggcga acctgatgag cgggggcgcg cagggcacca
     1381 ctcccgatcc gcagcagacc tggacgccgc cgccgcccga catgagccca ccaccgctgc
     1441 cgacgcccaa gacctactcc gaggcgaccg aatggatgca gaacaatccg ttctaccgcc
     1501 agtcgatcgc cattccgacc aactgcagcc aactgaagcc gatcgacgcg ctgaccgcgt
     1561 ccgagagcga gctcaacacg cacctgcaga acctcacctc gtgcctgttg caggtgtgga
     1621 aggcgccggt cgaggcggcc ggtttcgaga tgccgcggcc accggcaacg gtctacaacc
     1681 agccgatcca gaccgcgtgc ggcaagctgg acagcgtgaa cgcgtcctac tgcggcgccg
     1741 accagcgcat ctactacgcc aaaccgctac cgaagatctt tcccagagac ctgcaagggc
     1801 agaagttcct catggagatg gtgctgggcc acgagttcgg ccacgccatt caggcgcgca
     1861 cgggcatctc gatctcgagc agcgcatggg agcaacgggc gtccacgaag gcggcggcca
     1921 acgtgttctc gcggcgactc gaggtgcagg cggactgctt cgcgggcatg tggatccacg
     1981 cggtcggcga atcgcagggc ctgacctcca gcgatctgga cggcctgcgc atcatcgcct
     2041 acaacctggg cgatgacgtg ctgtcgggcc ggcagaacat cgactcgggc cacgggctgg
     2101 gcaagacgcg gcagcgctgg ttcgaaacgg ggctcaccgg aagcccgcag acgctgcagt
     2161 gcaacaccta ctcggttcca gacgcgcagg tgcgctgacg gctgcttggt cgagcgagca
     2221 cggacggggg cgcacggcgg gcggcgttca gcggcagccg aggtgtcgac gctcagtccg
     2281 tccccggctc ggccatcggg tggtggtgca gcgggcgtcc gacctcgctg aagcccaggc
     2341 gctggtacac ccctcgtccg gacggggtgg ctgacagcgt cgcaggcatg tggggggcca
     2401 ccgcgaccgc cgccatggtc agagccgagc cgatgccacg tccacgccgg gcgggcatca
     2461 ccgccaacga gccgatgttg ttcacccggg gcgtcaccac cgagagcgct gtaccgaccc
     2521 cttcgccgtc gatctcggcg atcagcaacg tcaatcccgg tagccgc
//
LOCUS       DAOSUG010000467         2533 bp    DNA     linear   ENV 10-JUN-2024
DEFINITION  MAG TPA_asm: Micropruina sp. isolate AMR_MDS_2195
            NODE_16574_length_2533_cov_4.502421, whole genome shotgun sequence.
ACCESSION   DAOSUG010000467 DAOSUG010000000
VERSION     DAOSUG010000467.1
DBLINK      BioProject: PRJNA925477
            BioSample: SAMN32864262
            Sequence Read Archive: SRR4236673
KEYWORDS    WGS; ENV; Metagenome Assembled Genome; MAG; Third Party Data; TPA;
            TPA:assembly.
SOURCE      Micropruina sp. (wastewater metagenome)
  ORGANISM  Micropruina sp.
            Bacteria; Bacillati; Actinomycetota; Actinomycetes;
            Propionibacteriales; Nocardioidaceae; Micropruina.
REFERENCE   1  (bases 1 to 2533)
  AUTHORS   Abdulkadir,N., Saraiva,J.P., Zhang,J., Stolte,S., Gillor,O.,
            Harms,H. and Rocha,U.
  TITLE     Genome-centric analyses of 165 metagenomes show that mobile genetic
            elements are crucial for the transmission of antimicrobial
            resistance genes to pathogens in activated sludge and wastewater
  JOURNAL   Microbiol Spectr 12 (3), e0291823 (2024)
   PUBMED   38289113
REFERENCE   2  (bases 1 to 2533)
  AUTHORS   Abdulkadir,N.
  TITLE     Direct Submission
  JOURNAL   Submitted (24-MAR-2023) Environmental Microbiology, Helmholtz
            Center for Environmental Research -UFZ, Permoserstr 15, Leipzig
            04318, Germany
COMMENT     The annotation was added by the NCBI Prokaryotic Genome Annotation
            Pipeline (PGAP). Information about PGAP can be found here:
            https://www.ncbi.nlm.nih.gov/genome/annotation_prok/
            
            ##Genome-Assembly-Data-START##
            Assembly Method        :: SPAdes v. 3.11.1
            Genome Representation  :: Full
            Expected Final Version :: Yes
            Genome Coverage        :: Not Applicable
            Sequencing Technology  :: Illumina MiSeq
            ##Genome-Assembly-Data-END##
            
            ##Genome-Annotation-Data-START##
            Annotation Provider               :: NCBI
            Annotation Date                   :: 05/04/2023 04:57:10
            Annotation Pipeline               :: NCBI Prokaryotic Genome
                                                 Annotation Pipeline (PGAP)
            Annotation Method                 :: Best-placed reference protein
                                                 set; GeneMarkS-2+
            Annotation Software revision      :: 6.5
            Features Annotated                :: Gene; CDS; rRNA; tRNA; ncRNA
            Genes (total)                     :: 2,776
            CDSs (total)                      :: 2,750
            Genes (coding)                    :: 2,714
            CDSs (with protein)               :: 2,714
            Genes (RNA)                       :: 26
            tRNAs                             :: 24
            ncRNAs                            :: 2
            Pseudo Genes (total)              :: 36
            CDSs (without protein)            :: 36
            Pseudo Genes (ambiguous residues) :: 0 of 36
            Pseudo Genes (frameshifted)       :: 2 of 36
            Pseudo Genes (incomplete)         :: 32 of 36
            Pseudo Genes (internal stop)      :: 2 of 36
            ##Genome-Annotation-Data-END##
FEATURES             Location/Qualifiers
     source          1..2533
                     /organism="Micropruina sp."
                     /mol_type="genomic DNA"
                     /submitter_seqid="NODE_16574_length_2533_cov_4.502421"
                     /isolate="AMR_MDS_2195"
                     /isolation_source="Activated sludge of municipal
                     wastewater treatment plants"
                     /db_xref="taxon:2737536"
                     /environmental_sample
                     /geo_loc_name="Canada"
                     /lat_lon="49.25 N 123.1 W"
                     /collection_date="2015-06-16"
                     /metagenome_source="wastewater metagenome"
                     /note="metagenomic"
     gene            1..413
                     /locus_tag="PLF56_12790"
                     /pseudo
     CDS             1..413
                     /locus_tag="PLF56_12790"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_002512901.1"
                     /note="incomplete; partial in the middle of a contig;
                     missing N-terminus and C-terminus; Derived by automated
                     computational analysis using gene prediction method:
                     Protein Homology.
                     GO_component: GO:0016020 - membrane [Evidence IEA];
                     GO_process: GO:0051301 - cell division [Evidence IEA]"
                     /pseudo
                     /codon_start=3
                     /transl_table=11
                     /product="FtsW/RodA/SpoVE family cell cycle protein"
     gene            779..2194
                     /locus_tag="PLF56_12795"
     CDS             779..2194
                     /locus_tag="PLF56_12795"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_002512900.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_component: GO:0016020 - membrane [Evidence IEA];
                     GO_function: GO:0008658 - penicillin binding [Evidence
                     IEA];
                     GO_process: GO:0009252 - peptidoglycan biosynthetic
                     process [Evidence IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="penicillin-binding protein 2"
                     /protein_id="HOQ54476.1"
                     /translation="MNRPIRRVAFVAMLMTFALLLNVCVSYLLRQDSLNANPQNRRVT
                     DAAFAQDRGAILVGNTAVAETVASKDQFKFQRRYPQGDLYAPVTGFFSYNFGSSELEQ
                     TYGAQLSGNDDSLFINRLIDLATGAKPRGASVQTTLNAKAQKAAADALGSKKGAVVAL
                     NPNTGAILAMVNSPSFNPNLLATHQLNDARAAWTKLTTDQAKPMSNRAVREIFPPGST
                     FKVIVSAAALEAGYELDSVLDTSSYRLPGTNTTINTTCGNSQTLKRALAVSCNSAFAR
                     LGVKLGADALREQAEKFGFGTKPLSDIASVASRFPDEIDTPQTGLASIGGYDVAATPL
                     QMAMVAAAVRNDGVVMQPYLVESVRSADLQILSQTRPSQLSVATSAANAAKLRQLMVG
                     VVEDGTGWRAKIAGVTVGGKTGTANSDNRRTPYAWFIAWADDPDVAICVFVQDAEMEA
                     TDVAGGRVAAPIAKAVIEALR"
     gene            2253..>2533
                     /locus_tag="PLF56_12800"
     CDS             2253..>2533
                     /locus_tag="PLF56_12800"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_016668352.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="serine/threonine protein kinase"
                     /protein_id="HOQ54477.1"
                     /translation="MADEPRVLGGRYEMGSPLGRGGMAEVRRARDTRLGRQVAVKLLR
                     VDLAGDPVFQARFRREAQASAGLNHPNIVAVYDTGEEKDPASGVSIPYIV"
BASE COUNT          412 a          938 c          822 g          361 t
ORIGIN      
        1 accagatcat ccagggccag ttcggcatgg cctggggcgg gctgttcggc accggctggg
       61 ggcttggccg gcccaacctg accccgctgg ccaagaacga cttcatcgcc gccgcgctcg
      121 gcgaagagct gggtgtggcc gggctgatgg cgatcatcgt gctgtacgga gtgatcgtga
      181 tgcgcggcct gcgcgcgtcg ctgagcgcga aggacccgtt cagcaaactg ctggcggccg
      241 gcctgtcgtt cgtcttcgcg ctgcaggtct tcgcaatcat cggcggcgtc acccggctgc
      301 tgccgctcac cggcctgacc acgcccttcg tgtcgcaggg cggctcgtcg ctggcggcca
      361 actacgccat gctcggcctg ctgatggtgg tcacccacca ggttcgtcgc ccccaggccc
      421 agacgtccga gccgttgcag tccctggccc aggacaccac ccaggtgatc agcgtgccgg
      481 cccgcaccgc acccgcggcg cggccccgtc cggcgccccc agccgagtcg ctgcccaccc
      541 ccgccggcgc ggacgaaccc acgacggccg ttccgacggc ccgcaccccc gagtccgtcc
      601 ccacggccgg cacccccgac ggcgagccga cgacggcggt tccgctcacc ggtatcccgg
      661 acgagccgac gctggcggtt ccgctcaccg gcaccccgga cgagcccacc caggccattc
      721 cccagggtgc ccctgcagcc tgggatcccg acgacaccaa cccgcaagga aggctgccgt
      781 gaaccgcccg atccgccgag tcgccttcgt ggcgatgctg atgaccttcg cgctgttgct
      841 caatgtctgc gtcagctatc tgctgcggca ggattcgctg aacgccaacc cgcagaaccg
      901 ccgggtcacc gatgccgcgt tcgcccagga ccggggcgcc attctggtcg gcaacaccgc
      961 ggtcgccgaa acggtggcca gcaaggacca gttcaagttt cagcgccgct acccccaagg
     1021 cgatctgtat gcgccggtca ccgggttctt cagctacaac ttcgggtcgt ccgagctcga
     1081 gcagacctac ggcgcgcagt tgtccggcaa cgacgattca ctgttcatca accgcttgat
     1141 cgacctggcc accggcgcca agccccgggg tgcgagcgtg cagaccacgc tgaacgccaa
     1201 ggctcagaag gccgccgccg acgcattggg ctccaagaag ggtgcggtgg tcgccctcaa
     1261 ccccaacacc ggcgccattc tggccatggt caactcgccc agcttcaacc ccaacctgct
     1321 ggccacccac cagctcaacg acgcccgcgc ggcctggacc aaactgacca ccgaccaggc
     1381 caagccgatg tcgaaccggg ccgtccgcga gatcttcccc cccggttcga ccttcaaggt
     1441 gatcgtgtcc gccgcggcgc tcgaggccgg ctacgagctc gacagcgtgc tcgacacctc
     1501 gtcgtaccgg ttgccgggca ccaacaccac gatcaacacc acctgcggca acagccagac
     1561 cctgaagcgg gcgctggcgg tgtcctgcaa ctcggccttc gcccggctgg gcgtcaaact
     1621 cggcgcggac gcgctgcgcg agcaggccga gaagttcggc ttcggcacca agccgctctc
     1681 ggacatcgcc tcggtggcca gcaggtttcc cgacgagatc gacacgccgc agaccggcct
     1741 ggcctccatc ggcggctatg acgtggcggc cacgccgctg cagatggcga tggtggcggc
     1801 ggccgtccgc aacgacgggg tggtgatgca gccctacctg gtcgagtcgg tgcgttcggc
     1861 cgatctgcag atcctgagcc agacccgtcc ctcgcagctc agcgtggcca ccagcgcggc
     1921 gaacgccgcg aagctgcgcc agctgatggt gggcgtggtc gaggacggca ccggctggcg
     1981 cgccaagatc gccggggtca ccgtcggcgg caagaccggc acggcgaact ccgacaacag
     2041 gcgtaccccg tacgcctggt tcatcgcctg ggccgacgat cccgacgtgg cgatctgcgt
     2101 cttcgtccag gacgccgaga tggaggcgac cgacgtggcc ggcggacggg tcgccgcacc
     2161 catcgccaag gccgtgatcg aggcgctgcg atgatgggcc gcacgcgggg tcggccacaa
     2221 tggaccccga tcgacgaaat gaggacgtgc tgatggccga cgaaccaagg gtgctcggcg
     2281 gacgctacga gatgggtagc ccgctgggcc gtggcggcat ggccgaggtg cggcgcgccc
     2341 gcgacacccg actcggacga caggtggcgg tcaagctgct gcgcgtcgat ctggccggcg
     2401 accccgtctt tcaggcccga ttccggcgcg aagcacaggc ctccgcaggg ctgaaccacc
     2461 ccaacatcgt ggccgtctac gacaccggtg aagagaagga cccggcctca ggggtctcca
     2521 ttccctacat cgt
//
LOCUS       DAOSUG010000468         2531 bp    DNA     linear   ENV 10-JUN-2024
DEFINITION  MAG TPA_asm: Micropruina sp. isolate AMR_MDS_2195
            NODE_16594_length_2531_cov_3.580775, whole genome shotgun sequence.
ACCESSION   DAOSUG010000468 DAOSUG010000000
VERSION     DAOSUG010000468.1
DBLINK      BioProject: PRJNA925477
            BioSample: SAMN32864262
            Sequence Read Archive: SRR4236673
KEYWORDS    WGS; ENV; Metagenome Assembled Genome; MAG; Third Party Data; TPA;
            TPA:assembly.
SOURCE      Micropruina sp. (wastewater metagenome)
  ORGANISM  Micropruina sp.
            Bacteria; Bacillati; Actinomycetota; Actinomycetes;
            Propionibacteriales; Nocardioidaceae; Micropruina.
REFERENCE   1  (bases 1 to 2531)
  AUTHORS   Abdulkadir,N., Saraiva,J.P., Zhang,J., Stolte,S., Gillor,O.,
            Harms,H. and Rocha,U.
  TITLE     Genome-centric analyses of 165 metagenomes show that mobile genetic
            elements are crucial for the transmission of antimicrobial
            resistance genes to pathogens in activated sludge and wastewater
  JOURNAL   Microbiol Spectr 12 (3), e0291823 (2024)
   PUBMED   38289113
REFERENCE   2  (bases 1 to 2531)
  AUTHORS   Abdulkadir,N.
  TITLE     Direct Submission
  JOURNAL   Submitted (24-MAR-2023) Environmental Microbiology, Helmholtz
            Center for Environmental Research -UFZ, Permoserstr 15, Leipzig
            04318, Germany
COMMENT     The annotation was added by the NCBI Prokaryotic Genome Annotation
            Pipeline (PGAP). Information about PGAP can be found here:
            https://www.ncbi.nlm.nih.gov/genome/annotation_prok/
            
            ##Genome-Assembly-Data-START##
            Assembly Method        :: SPAdes v. 3.11.1
            Genome Representation  :: Full
            Expected Final Version :: Yes
            Genome Coverage        :: Not Applicable
            Sequencing Technology  :: Illumina MiSeq
            ##Genome-Assembly-Data-END##
            
            ##Genome-Annotation-Data-START##
            Annotation Provider               :: NCBI
            Annotation Date                   :: 05/04/2023 04:57:10
            Annotation Pipeline               :: NCBI Prokaryotic Genome
                                                 Annotation Pipeline (PGAP)
            Annotation Method                 :: Best-placed reference protein
                                                 set; GeneMarkS-2+
            Annotation Software revision      :: 6.5
            Features Annotated                :: Gene; CDS; rRNA; tRNA; ncRNA
            Genes (total)                     :: 2,776
            CDSs (total)                      :: 2,750
            Genes (coding)                    :: 2,714
            CDSs (with protein)               :: 2,714
            Genes (RNA)                       :: 26
            tRNAs                             :: 24
            ncRNAs                            :: 2
            Pseudo Genes (total)              :: 36
            CDSs (without protein)            :: 36
            Pseudo Genes (ambiguous residues) :: 0 of 36
            Pseudo Genes (frameshifted)       :: 2 of 36
            Pseudo Genes (incomplete)         :: 32 of 36
            Pseudo Genes (internal stop)      :: 2 of 36
            ##Genome-Annotation-Data-END##
FEATURES             Location/Qualifiers
     source          1..2531
                     /organism="Micropruina sp."
                     /mol_type="genomic DNA"
                     /submitter_seqid="NODE_16594_length_2531_cov_3.580775"
                     /isolate="AMR_MDS_2195"
                     /isolation_source="Activated sludge of municipal
                     wastewater treatment plants"
                     /db_xref="taxon:2737536"
                     /environmental_sample
                     /geo_loc_name="Canada"
                     /lat_lon="49.25 N 123.1 W"
                     /collection_date="2015-06-16"
                     /metagenome_source="wastewater metagenome"
                     /note="metagenomic"
     gene            <1..196
                     /locus_tag="PLF56_12805"
     CDS             <1..196
                     /locus_tag="PLF56_12805"
                     /inference="COORDINATES: ab initio
                     prediction:GeneMarkS-2+"
                     /note="Derived by automated computational analysis using
                     gene prediction method: GeneMarkS-2+."
                     /codon_start=2
                     /transl_table=11
                     /product="hypothetical protein"
                     /protein_id="HOQ54478.1"
                     /translation="AILYSIIGVARLSKVYGELEAGHVRIESLSGAKPVAHDGEITDP
                     KDVVELSISDRRLIVYRATS"
     gene            complement(283..1539)
                     /locus_tag="PLF56_12810"
     CDS             complement(283..1539)
                     /locus_tag="PLF56_12810"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_014317774.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="integrase"
                     /protein_id="HOQ54479.1"
                     /translation="MASRAEITTRYAKAFKAADRRTKGRILDEVVSVTGWSRDNARRR
                     LTSAAQCPPGGGRQVAQRPRKQRANKFSYEAVKVLQRVWAASGGQCGKYLAASMDTQL
                     DGLERHGELVDGEGRYSASVRAELLAMSPATIDRYLRTAKATDQVRGVSTTKPSPLLR
                     SSIKIRKAGDEVEAEPGFFEGDTVAHCGPTLRGEFARSVNLTCVHTGWVFTRSTRNNA
                     HANILAALQAGVQEIPFAVTGLDFDNGGEFLNRAVIKWAAERDIYFTRSRPYKKNDQA
                     TIESKNNHLVRRYAFYYRYDTDEERHALNRLWKLVNDRLNYLTPTIKPVGWGENKAGR
                     RKRLYDKPQTPLSRLLAAGTLSPAQAHELTAYRDGLNPAALAREIADIQAVLLGLAKN
                     KTEQLYLATVPKALPDVRKGVRIRAG"
     gene            1738..>2531
                     /locus_tag="PLF56_12815"
     CDS             1738..>2531
                     /locus_tag="PLF56_12815"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_011755449.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="alpha/beta fold hydrolase"
                     /protein_id="HOQ54480.1"
                     /translation="MAGRKSLRVQTRTVHGHRRAYVLAGRGPALLLLHGVGCDHHTWD
                     PVIEHLSRRYTVIAPDLLGHGDSAKPRADYSLGGFANGMRDLLAILGIDRVTVVGHSF
                     GGGVAMQFAYQFPQYAERLVMVAPGGLGPEVNPILRGLTLPGGSTALAVAATPPVYAA
                     LRLVGGRAHAIGLPGTADIPGALGVLKGKADPAERDAFLHVLRAVVDWKGQVVSMTDR
                     AYLAREMPTCLVWGSRDTVLPVVHAQRARAVIPAARIEVIPGAGHFP"
BASE COUNT          342 a          877 c          879 g          433 t
ORIGIN      
        1 ggcgatcctg tactcgatca tcggcgtggc ccggctgagc aaggtctacg gcgagctgga
       61 ggccggccac gtgcgtatcg agtcgctgtc cggagccaag ccggtggccc acgacggcga
      121 gatcaccgat ccgaaagacg tggtggaact gtccatctcg gatcggcggc tgatcgtgta
      181 ccgggccacc agctgattgt ccggggggcc gcaatgcctc acgtcaagat gcccgcgtag
      241 gacgatccat gcctcatcta ggaatgcccg cgaagcaggg cgtcagccgg ccctgatccg
      301 gacgcctttg cgcacgtcgg gcagtgcctt ggggacggtc gcgaggtaga gctgctcggt
      361 cttgttcttg gccaggccca gcagcacggc ttgaatgtcg gcgatctcac gggcgagcgc
      421 ggccgggttg agcccgtccc ggtaggcggt cagctcgtgg gcttgcgccg gcgacagcgt
      481 gccggcggcc agcagccgac tcaacggggt ttgcggcttg tcgtacaggc gtttgcggcg
      541 accggccttg ttctcacccc agccgaccgg cttgatcgtc ggggtgaggt agttgagccg
      601 gtcgttgacc agcttccaga gccggttcaa cgcgtgccgc tcctcgtcgg tgtcgtaccg
      661 gtagtagaac gcgtaccggc ggaccaggtg gttgttcttc gactcgatcg tggcctggtc
      721 gttcttcttg tacggccggg accgggtgaa gtagatgtct cgctcggcgg cccatttgat
      781 gacggcccgg ttcaggaact cacctccgtt gtcgaagtcc agaccggtga ccgcgaaagg
      841 gatctcctgc accccggcct gcagcgcggc caggatgttg gcgtgcgcgt tgttgcgcgt
      901 cgagcgggtg aagacccacc cggtatgcac acaggtcagg ttcaccgagc gagcgaactc
      961 gccccgcagg gtcgggccgc aatgcgcaac cgtgtcgccc tcgaagaagc cgggctcggc
     1021 ctcgacctcg tcgcccgcct tacggatctt gatcgaggac cgcagcaacg gtgacggttt
     1081 cgtggtcgag acaccgcgga cctggtcggt ggccttcgcg gtccgcaggt agcggtcgat
     1141 cgtcgccggc gacatcgcga gcagctcggc ccgcaccgaa gcgctgtacc ggccctcgcc
     1201 gtcgaccagc tccccgtgcc gttccagccc gtccagttgc gtgtccatcg atgcggccag
     1261 gtacttgccg cactgcccac cggaagccgc ccagacccgc tggaggacct ttacggcctc
     1321 gtaggagaac ttgttcgccc gctgcttcct gggccgctgg gcgacctgtc ggccgccgcc
     1381 cggcgggcac tgcgcggcgc tggtcaaccg tcgccgggcg ttgtcccgcg accagcccgt
     1441 cacggacacc acctcgtcca ggatccggcc cttcgtacgc ctgtccgcag ccttgaacgc
     1501 cttggcgtac ctggtggtga tctccgcccg agacgccatc gacaacccac tctccatgcc
     1561 ccagcacggt caccgctacg cgggcattcc tacgtgaggc acggagagcg tttcgcgggc
     1621 agttctggtg agtcttgtcg cgccctggcc ccggacccgg gaccggggtt agtctgcctg
     1681 atcagaccgt ttctgacggt cctgacaact tcgctcccca cctccgggag ggctgccgtg
     1741 gccggtcgca agtcgttgcg ggtacagacc cgcaccgtgc acgggcaccg ccgcgcctat
     1801 gtgctagccg gccggggccc ggcgctgctg ttgctgcacg gcgtcggctg cgatcaccac
     1861 acctgggacc cggtgatcga gcacctgtcc cgccggtaca cggtgatcgc gccggacctg
     1921 ctggggcacg gggactcggc caagccgcgg gccgactaca gcctgggcgg cttcgccaac
     1981 ggcatgcgtg acctgctggc cattctgggc atcgaccggg tcacggtggt cgggcacagc
     2041 ttcggtggcg gggtggccat gcagttcgcc taccagttcc cgcagtacgc cgagcggttg
     2101 gtgatggtcg ccccgggcgg gctcggcccg gaggtgaacc cgatcctgcg cgggctgacc
     2161 ctgcccggcg gctcgaccgc cctcgcggtg gccgccaccc cgccggtcta cgccgccctg
     2221 cggctggtcg gcggtcgcgc ccacgccatc gggttgcccg gcaccgccga catccctggc
     2281 gcgctgggcg tgctgaaggg caaggccgac ccggccgagc gggacgcgtt cctccacgtg
     2341 ctgcgggccg tcgtggactg gaaggggcag gtcgtcagca tgaccgaccg ggcctacctg
     2401 gcccgggaga tgccgacctg cctggtctgg ggttcccggg acacggtgct gccggtggtg
     2461 catgcgcagc gcgcccgcgc ggtcatcccg gcggcccgga tcgaggtcat cccgggggcc
     2521 gggcactttc c
//
LOCUS       DAOSUG010000469         2513 bp    DNA     linear   ENV 10-JUN-2024
DEFINITION  MAG TPA_asm: Micropruina sp. isolate AMR_MDS_2195
            NODE_16739_length_2513_cov_3.774207, whole genome shotgun sequence.
ACCESSION   DAOSUG010000469 DAOSUG010000000
VERSION     DAOSUG010000469.1
DBLINK      BioProject: PRJNA925477
            BioSample: SAMN32864262
            Sequence Read Archive: SRR4236673
KEYWORDS    WGS; ENV; Metagenome Assembled Genome; MAG; Third Party Data; TPA;
            TPA:assembly.
SOURCE      Micropruina sp. (wastewater metagenome)
  ORGANISM  Micropruina sp.
            Bacteria; Bacillati; Actinomycetota; Actinomycetes;
            Propionibacteriales; Nocardioidaceae; Micropruina.
REFERENCE   1  (bases 1 to 2513)
  AUTHORS   Abdulkadir,N., Saraiva,J.P., Zhang,J., Stolte,S., Gillor,O.,
            Harms,H. and Rocha,U.
  TITLE     Genome-centric analyses of 165 metagenomes show that mobile genetic
            elements are crucial for the transmission of antimicrobial
            resistance genes to pathogens in activated sludge and wastewater
  JOURNAL   Microbiol Spectr 12 (3), e0291823 (2024)
   PUBMED   38289113
REFERENCE   2  (bases 1 to 2513)
  AUTHORS   Abdulkadir,N.
  TITLE     Direct Submission
  JOURNAL   Submitted (24-MAR-2023) Environmental Microbiology, Helmholtz
            Center for Environmental Research -UFZ, Permoserstr 15, Leipzig
            04318, Germany
COMMENT     The annotation was added by the NCBI Prokaryotic Genome Annotation
            Pipeline (PGAP). Information about PGAP can be found here:
            https://www.ncbi.nlm.nih.gov/genome/annotation_prok/
            
            ##Genome-Assembly-Data-START##
            Assembly Method        :: SPAdes v. 3.11.1
            Genome Representation  :: Full
            Expected Final Version :: Yes
            Genome Coverage        :: Not Applicable
            Sequencing Technology  :: Illumina MiSeq
            ##Genome-Assembly-Data-END##
            
            ##Genome-Annotation-Data-START##
            Annotation Provider               :: NCBI
            Annotation Date                   :: 05/04/2023 04:57:10
            Annotation Pipeline               :: NCBI Prokaryotic Genome
                                                 Annotation Pipeline (PGAP)
            Annotation Method                 :: Best-placed reference protein
                                                 set; GeneMarkS-2+
            Annotation Software revision      :: 6.5
            Features Annotated                :: Gene; CDS; rRNA; tRNA; ncRNA
            Genes (total)                     :: 2,776
            CDSs (total)                      :: 2,750
            Genes (coding)                    :: 2,714
            CDSs (with protein)               :: 2,714
            Genes (RNA)                       :: 26
            tRNAs                             :: 24
            ncRNAs                            :: 2
            Pseudo Genes (total)              :: 36
            CDSs (without protein)            :: 36
            Pseudo Genes (ambiguous residues) :: 0 of 36
            Pseudo Genes (frameshifted)       :: 2 of 36
            Pseudo Genes (incomplete)         :: 32 of 36
            Pseudo Genes (internal stop)      :: 2 of 36
            ##Genome-Annotation-Data-END##
FEATURES             Location/Qualifiers
     source          1..2513
                     /organism="Micropruina sp."
                     /mol_type="genomic DNA"
                     /submitter_seqid="NODE_16739_length_2513_cov_3.774207"
                     /isolate="AMR_MDS_2195"
                     /isolation_source="Activated sludge of municipal
                     wastewater treatment plants"
                     /db_xref="taxon:2737536"
                     /environmental_sample
                     /geo_loc_name="Canada"
                     /lat_lon="49.25 N 123.1 W"
                     /collection_date="2015-06-16"
                     /metagenome_source="wastewater metagenome"
                     /note="metagenomic"
     gene            1..1530
                     /locus_tag="PLF56_12820"
                     /pseudo
     CDS             1..1530
                     /locus_tag="PLF56_12820"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_007400926.1"
                     /note="internal stop; incomplete; missing N-terminus;
                     Derived by automated computational analysis using gene
                     prediction method: Protein Homology.
                     GO_function: GO:0004470 - malic enzyme activity [Evidence
                     IEA];
                     GO_function: GO:0004471 - malate dehydrogenase
                     (decarboxylating) (NAD+) activity [Evidence IEA];
                     GO_function: GO:0046872 - metal ion binding [Evidence
                     IEA];
                     GO_function: GO:0051287 - NAD binding [Evidence IEA]"
                     /pseudo
                     /codon_start=1
                     /transl_table=11
                     /product="NAD-dependent malic enzyme"
     gene            complement(1667..>2513)
                     /locus_tag="PLF56_12825"
     CDS             complement(1667..>2513)
                     /locus_tag="PLF56_12825"
                     /EC_number="1.-.-.-"
                     /inference="COORDINATES: protein motif:HMM:TIGR03558.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_function: GO:0010181 - FMN binding [Evidence IEA];
                     GO_function: GO:0016491 - oxidoreductase activity
                     [Evidence IEA]"
                     /codon_start=2
                     /transl_table=11
                     /product="LLM class flavin-dependent oxidoreductase"
                     /protein_id="HOQ54481.1"
                     /translation="AEHHNMPTIASSATAVLIAHVAAHTSTIRLGAGGVMLPNHAPLV
                     IAEQFGTLAELHPGRIDLGLGRAPGTDQVTVRALRRDPAAADTFPRDVQELQGFLDES
                     LVPGVSATPGAGTRVPLYILGSSLFGAQLAAALGLPYAFASHFAPDALEAAVATYRRD
                     FRPSAQLAQPYVIAGAGVIAADTEAEARGLFELTRRARVRMFLGRGRTLTDDDVELLL
                     DGPGGVQVDHMLQYTGVGTRDQVRQWLTGFAAHADADELILVSSAIERPAALRSLELV
                     APDRH"
BASE COUNT          441 a          844 c          838 g          390 t
ORIGIN      
        1 gatcagcaag cgacacgcac ctacgcacag ttgcagaagc aacccaccga tctggcgaaa
       61 tacatctacc tcgaccaact ccatgaacgt aacgaggtgt tgtatttccg ggtcatcgcc
      121 gatcatctcg ccgacctgct gccggtcgtg tacgacccga ccatcgggga cgcgatcgag
      181 cagtggtcga aggattaccg gcagtcccgg gccgtgtact tgtcgatcga tcatgtcgac
      241 gacatcgaag cgtccttcgc gaccctcggg ctgggtcaag tggatgtcga tctgatcgtc
      301 gtttccgatg cggaggagat ccttgggatc ggtgactggg gtgtcaacgg caccgacatc
      361 gccgtgggaa agctggcgat ctacactgca gccgcgggca tcgacccgtc ccgggtgatt
      421 gcggtgaacc tggatgtcgg cacggacaac gaatcgctcc tgaacgaccc ggcctatctc
      481 ggcaatcggc acagccgcat ccgcggtgaa aggtatgacg ccttcatcgc cgcgtatctg
      541 cagacggcga agaagctgtt tcccaacgcg ttgctgcact tcgaggattt cggcccctcc
      601 aatgcccgcc gcatcctcat cgacaacgcc gacgattacc gcatcttcaa cgatgacatg
      661 cagggcaccg gtgcgatcgt gatggcggcg gtgatctcgg gcttgaaggt caccaagcag
      721 accttcgccg accagcgcct ggtcgtcttc ggtgccggca cggcgggtac cggcatggcc
      781 gaccagatct cggccgcgat gcagcgcggc ggcctcagcg agaaggatgc ccgaagccgg
      841 gtgtggttga tcgacaagca gggcctcgtc accgatgaca tgcagaacct ccccgactat
      901 caacagcctt acgcacgccc ggccgcagag gtgagcgaat ggggtgggca cccgatcgat
      961 ctgctgaccg tcgtgcagca ggtgaagccc accatcctga tcggcacctc gaccgcgcac
     1021 ggcgcgttca cccgagacgt gatcgaagca ctcagcgcgg gtgtcgagcg tcccatcctg
     1081 ttgccgctgt cgaacccgac cagcaggatc gaggtgatgc cgcaggacgc gatcccgtgg
     1141 tcgaagggcc aggcgttgat ggcgacggga attcccatcg ctccggtgta gtacgacggc
     1201 gtccgatacc agatcgggca gggcaacaac gcgctgctgt atccgggact cggattggga
     1261 acgatcgttt gcggggccgc acgggtcacc gacggcatgc tcctcgccgc cgcggaggcg
     1321 gtcgccggac aggtcgacgt ctccgaacgg ggagcctcgc tgctgccgga cgtggcaaac
     1381 ctgagggcct catccgcgac tgtcgccatc gctgtggcca aggcggcagc cgccgacggc
     1441 gttgccacgg tccagcacga cgatctcgtt caggccgtcc agaccgcgat gtggcaaccc
     1501 gtctacccgg cctcactccc gggcgcctga cgccgcaacc tcgcacgctt cgcgccggcc
     1561 gatgcgccgt gggcggttgt tggtcgacat tccggcaggc tgctcgacgg ccgcccgcgc
     1621 tgtgcgggtc agccggtctc tacggcctgc cggccaccaa aagccgctag tggcggtccg
     1681 gtgcgaccag ttcgagggag cgaagtgccg ccggtcgctc gatcgcagac gacaccagga
     1741 tcagctcgtc cgcgtcggca tgggcggcga agccggtcaa ccactgccgc acctggtcgc
     1801 gggtgccgac gccggtgtac tgcagcatgt ggtccacctg gacaccgccc ggcccgtcaa
     1861 gcagcagttc gacgtcgtca tcggtgagcg tcctcccccg acccaggaac atgcggacgc
     1921 gcgcccgccg ggtcaactcg aagagcccac gcgcttcggc ctcggtgtcg gcggcgatca
     1981 cccccgcacc ggcgatcacg tagggctgtg ccaactgcgc cgacggacgg aagtcgcgcc
     2041 ggtaggtggc gacggctgcc tccagtgcat cgggcgcgaa atgcgacgcg aacgcgtagg
     2101 gcaacccgag ggctgcggcc agctgggcgc cgaacaagga agacccgaga atgtagagcg
     2161 gtacccgcgt gccggcaccg ggggtggcgc tcaccccggg caccagcgac tcgtccaaga
     2221 atccctgcaa ctcctggacg tcgcgcggaa aggtgtcggc agcggccgga tcgcggcgca
     2281 gagcccgcac cgtcacctgg tcggtgcccg gcgcccggcc caggccgaga tcgatccggc
     2341 ccgggtgcag ttcggcgagc gtgccgaact gctcggcgat caccagcggg gcgtggttgg
     2401 gcagcatcac cccgccggca cccagccgaa tcgtggaggt gtgggccgcg acgtgcgcga
     2461 tcagcaccgc cgtggccgac gacgcgatgg tcggcatgtt gtgatgctcg gcg
//
LOCUS       DAOSUG010000470         2512 bp    DNA     linear   ENV 10-JUN-2024
DEFINITION  MAG TPA_asm: Micropruina sp. isolate AMR_MDS_2195
            NODE_16745_length_2512_cov_5.045991, whole genome shotgun sequence.
ACCESSION   DAOSUG010000470 DAOSUG010000000
VERSION     DAOSUG010000470.1
DBLINK      BioProject: PRJNA925477
            BioSample: SAMN32864262
            Sequence Read Archive: SRR4236673
KEYWORDS    WGS; ENV; Metagenome Assembled Genome; MAG; Third Party Data; TPA;
            TPA:assembly.
SOURCE      Micropruina sp. (wastewater metagenome)
  ORGANISM  Micropruina sp.
            Bacteria; Bacillati; Actinomycetota; Actinomycetes;
            Propionibacteriales; Nocardioidaceae; Micropruina.
REFERENCE   1  (bases 1 to 2512)
  AUTHORS   Abdulkadir,N., Saraiva,J.P., Zhang,J., Stolte,S., Gillor,O.,
            Harms,H. and Rocha,U.
  TITLE     Genome-centric analyses of 165 metagenomes show that mobile genetic
            elements are crucial for the transmission of antimicrobial
            resistance genes to pathogens in activated sludge and wastewater
  JOURNAL   Microbiol Spectr 12 (3), e0291823 (2024)
   PUBMED   38289113
REFERENCE   2  (bases 1 to 2512)
  AUTHORS   Abdulkadir,N.
  TITLE     Direct Submission
  JOURNAL   Submitted (24-MAR-2023) Environmental Microbiology, Helmholtz
            Center for Environmental Research -UFZ, Permoserstr 15, Leipzig
            04318, Germany
COMMENT     The annotation was added by the NCBI Prokaryotic Genome Annotation
            Pipeline (PGAP). Information about PGAP can be found here:
            https://www.ncbi.nlm.nih.gov/genome/annotation_prok/
            
            ##Genome-Assembly-Data-START##
            Assembly Method        :: SPAdes v. 3.11.1
            Genome Representation  :: Full
            Expected Final Version :: Yes
            Genome Coverage        :: Not Applicable
            Sequencing Technology  :: Illumina MiSeq
            ##Genome-Assembly-Data-END##
            
            ##Genome-Annotation-Data-START##
            Annotation Provider               :: NCBI
            Annotation Date                   :: 05/04/2023 04:57:10
            Annotation Pipeline               :: NCBI Prokaryotic Genome
                                                 Annotation Pipeline (PGAP)
            Annotation Method                 :: Best-placed reference protein
                                                 set; GeneMarkS-2+
            Annotation Software revision      :: 6.5
            Features Annotated                :: Gene; CDS; rRNA; tRNA; ncRNA
            Genes (total)                     :: 2,776
            CDSs (total)                      :: 2,750
            Genes (coding)                    :: 2,714
            CDSs (with protein)               :: 2,714
            Genes (RNA)                       :: 26
            tRNAs                             :: 24
            ncRNAs                            :: 2
            Pseudo Genes (total)              :: 36
            CDSs (without protein)            :: 36
            Pseudo Genes (ambiguous residues) :: 0 of 36
            Pseudo Genes (frameshifted)       :: 2 of 36
            Pseudo Genes (incomplete)         :: 32 of 36
            Pseudo Genes (internal stop)      :: 2 of 36
            ##Genome-Annotation-Data-END##
FEATURES             Location/Qualifiers
     source          1..2512
                     /organism="Micropruina sp."
                     /mol_type="genomic DNA"
                     /submitter_seqid="NODE_16745_length_2512_cov_5.045991"
                     /isolate="AMR_MDS_2195"
                     /isolation_source="Activated sludge of municipal
                     wastewater treatment plants"
                     /db_xref="taxon:2737536"
                     /environmental_sample
                     /geo_loc_name="Canada"
                     /lat_lon="49.25 N 123.1 W"
                     /collection_date="2015-06-16"
                     /metagenome_source="wastewater metagenome"
                     /note="metagenomic"
     gene            416..1588
                     /gene="sucC"
                     /locus_tag="PLF56_12830"
     CDS             416..1588
                     /gene="sucC"
                     /locus_tag="PLF56_12830"
                     /EC_number="6.2.1.5"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_020392419.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_function: GO:0003824 - catalytic activity [Evidence
                     IEA];
                     GO_function: GO:0005524 - ATP binding [Evidence IEA];
                     GO_function: GO:0046872 - metal ion binding [Evidence
                     IEA];
                     GO_process: GO:0006099 - tricarboxylic acid cycle
                     [Evidence IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="ADP-forming succinate--CoA ligase subunit beta"
                     /protein_id="HOQ54482.1"
                     /translation="MDLFEYQARDLFEAHGVPVLRGIVARTPDEAEAAARSLGTPVVV
                     VKAQVKTGGRGKAGGVKLARSPQEARDRASEILGLVINGHTVEQVMVTEGADIAEEYY
                     FGLLLDRANRTYLAMCSVEGGMDIETLAEERPEALARVEVDPIVGLDAAKADEIVAAA
                     GFAEADRAEVARVLVLLGEVYRDNDATLVEVNPLVKTGEGRILALDGKVSLDANASFR
                     HSDWTQLADESRDDPLEIRAREKNLNYVKLDGSVGIIGNGAGLVMSTLDVVAGAGKEF
                     PGQPTPANFLDIGGGASAQVMADGLDIILSDSQVKVVFVNVFGGITACSEVAKGILAA
                     LDILGEKASKPIVVRLDGNAVEIGRHILTEAANPLVTMELTMDSAARKAAELASKN"
     gene            1603..2493
                     /gene="sucD"
                     /locus_tag="PLF56_12835"
     CDS             1603..2493
                     /gene="sucD"
                     /locus_tag="PLF56_12835"
                     /EC_number="6.2.1.5"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_012918932.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_function: GO:0003824 - catalytic activity [Evidence
                     IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="succinate--CoA ligase subunit alpha"
                     /protein_id="HOQ54483.1"
                     /translation="MTIWLDQKSKVLVQGMTGSEGRKHTQRMISSGTRVVGGVTPGKG
                     GQTVTFEEDPVAVYDSVAEAKAATGANVSVIFVPAAYTRKAVFEAVDAGIELVVVITE
                     GVPVKDTAEFFEYADKSGTTRLIGPNCPGLISPGHSNVGIIPASIAPKPGRIGLVSKS
                     GTLTYQMMFELADIGFSTAVGIGGDPIIGTTHIDALEAFENDPDTDCIVMIGEIGGDA
                     EERAAAYIAEHVSKPVVGYVAGFTAPEGKTMGHAGAIVSGSKGTAQAKKAALEAAGVK
                     VGRTPSETASLVRELMESTK"
BASE COUNT          453 a          813 c          882 g          364 t
ORIGIN      
        1 taccggtgcg gcggacgacg agcgggtcgc ctggccggcc aagccgaccg cgagagcacc
       61 gatggacaaa gcagcgaccc cggtacgcac cacccgtgac cggatccagc tggtcgtctg
      121 gggctgagcc tgatttcggc gcacgcgggt gtctcctcgt tctgcaacag tggtaggtct
      181 tccgagaacc ggaatcctgt atgtacctta gcatttctta ggaagattct cagaatcgga
      241 cgagaggggc ctgggaaggc cctcgccgcg accccatgcg gagcccccga acaccgaacc
      301 agcggcccgg cgcgacaact gctatggccg gcagtgtgcc ctggccaccc tcgtccgact
      361 atcgtgacca gtgcctgccg gtcaggcgtc gtgacccaga cggacggagt tgtcggtgga
      421 tctattcgag taccaggcgc gggacctttt cgaggcccac ggtgtaccgg tgttgcgggg
      481 catcgtggcc cgcacgcccg acgaggcgga ggccgcggcc cgctcgctgg gcacgcccgt
      541 ggtggtcgtc aaggcgcagg tgaagaccgg cggacgcggc aaggcgggcg gcgtgaagct
      601 ggcccgctcg ccgcaggagg cgcgcgaccg ggcgtccgag attctgggcc tggtgatcaa
      661 tgggcacacc gtcgagcagg tcatggtcac cgagggggcc gacatcgccg aggagtacta
      721 cttcggcctg ctgctcgacc gggccaaccg cacctacctg gccatgtgct cggtcgaggg
      781 cggcatggac atcgagacgc tcgccgagga gcgtcccgag gcgttggcgc gggtcgaggt
      841 cgacccgatc gtcggtcttg acgccgccaa ggccgacgag atcgtggcgg ctgcgggctt
      901 cgcggaggcc gatcgggccg aggtggcccg ggtgctggtg ttgctgggcg aggtctatcg
      961 cgacaacgac gccacgctgg tcgaggtcaa cccgctggtc aaaaccggcg agggccgcat
     1021 cctggcgctg gacggcaagg tctcgctcga cgccaatgcg tccttccggc acagcgactg
     1081 gacgcagctg gccgacgagt cgcgcgacga cccgctcgag atcagggcac gtgagaagaa
     1141 cctcaactac gtcaagctcg acggcagcgt cggcatcatc ggcaacggcg ccggtctggt
     1201 gatgagcacg ctcgacgtgg tcgccggggc cggcaaggag tttccggggc agccgacgcc
     1261 ggccaatttt ctcgacatcg gcggcggcgc gtccgcgcag gtgatggcgg acggactcga
     1321 catcatcctg tccgactcgc aggtgaaggt ggttttcgtc aacgtgttcg gcggcatcac
     1381 cgcatgctcc gaggtggcca agggcatcct cgccgccctc gacattctgg gcgagaaggc
     1441 gagcaagccg atcgtcgtcc ggctggacgg caacgcggtc gagatcggcc ggcacatact
     1501 caccgaagcc gcgaacccgc tggtgacgat ggaactcacc atggactcgg ccgcccgcaa
     1561 ggccgccgaa ctggccagca agaactgaag ggacgacgtt tcatgacgat ctggctggac
     1621 cagaaatcca aggtgctcgt gcagggcatg accggctcgg agggccgcaa gcacacccaa
     1681 cgcatgatca gctccggcac ccgagtggtg ggcggcgtca cccccggcaa gggtgggcag
     1741 acggtgacct tcgaagagga cccggtcgcc gtctacgaca gtgtcgccga ggccaaggcg
     1801 gccaccggcg cgaacgtctc ggtgatcttc gtgcccgccg cctacaccag gaaggcggtc
     1861 ttcgaggccg tcgatgcggg catcgaactg gtggtggtga tcaccgaggg cgtgccggtc
     1921 aaggacaccg ccgagttctt cgagtacgcc gacaagagcg gcaccacgcg gctgatcgga
     1981 cccaactgtc ccgggctgat cagccccgga cactccaatg tgggcatcat tccggccagc
     2041 atcgcgccca agcccggacg catcgggctg gtcagcaagt cgggcaccct cacctaccag
     2101 atgatgttcg agctggccga catcggcttc tcgacggcgg tcggcatcgg gggtgacccg
     2161 atcatcggca ccacgcacat cgacgcactc gaagcgttcg agaacgatcc cgacaccgac
     2221 tgcatcgtga tgatcggcga gatcggcggc gacgccgaag agcgggccgc ggcctacatc
     2281 gccgagcacg tcagcaagcc ggtggtgggc tatgtggccg gcttcaccgc tcccgagggc
     2341 aagacgatgg gccatgccgg cgccatcgtg tcgggctcga agggcaccgc acaggccaag
     2401 aaggcggcgc tggaggcggc cggggtcaag gtcggacgca ccccgagtga gaccgcctcg
     2461 ctggtgcgcg aactgatgga aagcacgaag taactgcgcg cgcagaccga gc
//
LOCUS       DAOSUG010000471         2506 bp    DNA     linear   ENV 10-JUN-2024
DEFINITION  MAG TPA_asm: Micropruina sp. isolate AMR_MDS_2195
            NODE_16813_length_2506_cov_3.324765, whole genome shotgun sequence.
ACCESSION   DAOSUG010000471 DAOSUG010000000
VERSION     DAOSUG010000471.1
DBLINK      BioProject: PRJNA925477
            BioSample: SAMN32864262
            Sequence Read Archive: SRR4236673
KEYWORDS    WGS; ENV; Metagenome Assembled Genome; MAG; Third Party Data; TPA;
            TPA:assembly.
SOURCE      Micropruina sp. (wastewater metagenome)
  ORGANISM  Micropruina sp.
            Bacteria; Bacillati; Actinomycetota; Actinomycetes;
            Propionibacteriales; Nocardioidaceae; Micropruina.
REFERENCE   1  (bases 1 to 2506)
  AUTHORS   Abdulkadir,N., Saraiva,J.P., Zhang,J., Stolte,S., Gillor,O.,
            Harms,H. and Rocha,U.
  TITLE     Genome-centric analyses of 165 metagenomes show that mobile genetic
            elements are crucial for the transmission of antimicrobial
            resistance genes to pathogens in activated sludge and wastewater
  JOURNAL   Microbiol Spectr 12 (3), e0291823 (2024)
   PUBMED   38289113
REFERENCE   2  (bases 1 to 2506)
  AUTHORS   Abdulkadir,N.
  TITLE     Direct Submission
  JOURNAL   Submitted (24-MAR-2023) Environmental Microbiology, Helmholtz
            Center for Environmental Research -UFZ, Permoserstr 15, Leipzig
            04318, Germany
COMMENT     The annotation was added by the NCBI Prokaryotic Genome Annotation
            Pipeline (PGAP). Information about PGAP can be found here:
            https://www.ncbi.nlm.nih.gov/genome/annotation_prok/
            
            ##Genome-Assembly-Data-START##
            Assembly Method        :: SPAdes v. 3.11.1
            Genome Representation  :: Full
            Expected Final Version :: Yes
            Genome Coverage        :: Not Applicable
            Sequencing Technology  :: Illumina MiSeq
            ##Genome-Assembly-Data-END##
            
            ##Genome-Annotation-Data-START##
            Annotation Provider               :: NCBI
            Annotation Date                   :: 05/04/2023 04:57:10
            Annotation Pipeline               :: NCBI Prokaryotic Genome
                                                 Annotation Pipeline (PGAP)
            Annotation Method                 :: Best-placed reference protein
                                                 set; GeneMarkS-2+
            Annotation Software revision      :: 6.5
            Features Annotated                :: Gene; CDS; rRNA; tRNA; ncRNA
            Genes (total)                     :: 2,776
            CDSs (total)                      :: 2,750
            Genes (coding)                    :: 2,714
            CDSs (with protein)               :: 2,714
            Genes (RNA)                       :: 26
            tRNAs                             :: 24
            ncRNAs                            :: 2
            Pseudo Genes (total)              :: 36
            CDSs (without protein)            :: 36
            Pseudo Genes (ambiguous residues) :: 0 of 36
            Pseudo Genes (frameshifted)       :: 2 of 36
            Pseudo Genes (incomplete)         :: 32 of 36
            Pseudo Genes (internal stop)      :: 2 of 36
            ##Genome-Annotation-Data-END##
FEATURES             Location/Qualifiers
     source          1..2506
                     /organism="Micropruina sp."
                     /mol_type="genomic DNA"
                     /submitter_seqid="NODE_16813_length_2506_cov_3.324765"
                     /isolate="AMR_MDS_2195"
                     /isolation_source="Activated sludge of municipal
                     wastewater treatment plants"
                     /db_xref="taxon:2737536"
                     /environmental_sample
                     /geo_loc_name="Canada"
                     /lat_lon="49.25 N 123.1 W"
                     /collection_date="2015-06-16"
                     /metagenome_source="wastewater metagenome"
                     /note="metagenomic"
     gene            complement(<1..446)
                     /locus_tag="PLF56_12840"
     CDS             complement(<1..446)
                     /locus_tag="PLF56_12840"
                     /inference="COORDINATES: ab initio
                     prediction:GeneMarkS-2+"
                     /note="Derived by automated computational analysis using
                     gene prediction method: GeneMarkS-2+."
                     /codon_start=1
                     /transl_table=11
                     /product="hypothetical protein"
                     /protein_id="HOQ54484.1"
                     /translation="MTGPRRIAEAPVSPGRARVTSALRAVGVVVGLMAQVILALLGWT
                     VVNESEDHQPDLTMALWCLLGTCYSLVMVIALSIDSRHEGGRRPTRLQANMVVRWTSL
                     IASLISGMVGLVASFMVVVGGPDAETTLAYKVVGIWAVCVGIQRFAV"
     gene            complement(798..1739)
                     /gene="pflA"
                     /locus_tag="PLF56_12845"
     CDS             complement(798..1739)
                     /gene="pflA"
                     /locus_tag="PLF56_12845"
                     /EC_number="1.97.1.4"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_016456207.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_function: GO:0043365 -
                     [formate-C-acetyltransferase]-activating enzyme activity
                     [Evidence IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="pyruvate formate-lyase-activating protein"
                     /protein_id="HOQ54485.1"
                     /translation="MTFAVAEAGPLDEPGRSVIPGRSVAPGETLPTPGLPGSGLTPPP
                     PVRATMLGGIPVADHLSRSELLLAQRTGEVGSVHSWELVTAVDGPGTRMTTFLAGCPL
                     RCLYCHNPDTMAANRGVPVLADDLMARITRYRGVFRATGGGITLSGGEVLQQPAFAGR
                     ILRAAKRLGIHTAIDTSGFLGRNADDELLADVDLVLLDVKAGDEPTYHRVTGRRLQPT
                     LDFGRRLDTLGIETWIRFVLVPGLTDNPATIEPIADYAASLGCVSRVEVLPFHQMGRD
                     KWAEIGMSYELDGTEPPSAELVESTRQIFRDRGLTVY"
     gene            complement(1736..2194)
                     /locus_tag="PLF56_12850"
     CDS             complement(1736..2194)
                     /locus_tag="PLF56_12850"
                     /inference="COORDINATES: protein motif:HMM:NF013399.2"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_function: GO:0003824 - catalytic activity [Evidence
                     IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="glycine radical domain-containing protein"
                     /protein_id="HOQ54486.1"
                     /translation="MLTITSNVVYGKATGAFPCGHEAGTPFAPGANPENGADTHGMLA
                     SMMSVGKLSYDDALDGISLTNTITPPALGRTESERVANLVGILDAGMGEGLYHANINV
                     LNKETLLDAMEYPENYPQLTIRVSGYAVNFVKLTREQQLDVLSRTFHESV"
     gene            complement(2174..2506)
                     /locus_tag="PLF56_12855"
                     /pseudo
     CDS             complement(2174..2506)
                     /locus_tag="PLF56_12855"
                     /inference="COORDINATES: protein motif:HMM:NF014900.2"
                     /note="incomplete; partial in the middle of a contig;
                     missing N-terminus and C-terminus; Derived by automated
                     computational analysis using gene prediction method:
                     Protein Homology.
                     GO_function: GO:0003824 - catalytic activity [Evidence
                     IEA]"
                     /pseudo
                     /codon_start=1
                     /transl_table=11
                     /product="pyruvate formate lyase family protein"
BASE COUNT          386 a          840 c          872 g          408 t
ORIGIN      
        1 accgcaaagc gctggatgcc cacgcaaacg gcccagatgc cgaccacctt gtacgccagc
       61 gtggtttcgg cgtccggccc accgaccacg accatgaagg aggcgaccag gccgaccatg
      121 cccgagatca gcgaggcgat caacgaggtc cagcgcacca ccatgttggc ctgcagccgg
      181 gtgggccggc gtccaccctc gtggcggctg tcgatcgaca gggcgatcac catcaccagc
      241 gagtagcagg ttcccagtag gcaccagagc gccatcgtca ggtcgggttg gtgatcctcg
      301 gactcgttga ccaccgtcca gccgagcagc gccaggatca cctgcgccat gagtccgacg
      361 acgacgccga cggcgcgcag tgcgctcgtc acccgggccc gtcccggtga aacgggcgcc
      421 tcggcgattc ggcgcggccc agtcacgtcg ctgaccctac cggcccagtt cggtccggcc
      481 gtgcgcgggg ttcaccacgt gccgcccggt ggtgcctcag cgggcggcgg ctgcctcatc
      541 ggccgcacga ccgacgagtg cctgttcagg tgagtggctg cgatggggtc tatgttgaca
      601 tatcgacata gaccctcgtc atcctcgccg cccctgaacc ggccgcctgt ccaggcagcc
      661 ggcgacgtgc aaaactgcac catatttggc agggtcttcg ccttattgag gcgaccagcc
      721 ccacagatct ggtgcagttt cacggctgac cgggcccgtc cccatgcgcc gcccgggaca
      781 gtcgcgtcac gacccgttca gtagacagtc agccctcggt cgcggaagat ctgccgggtc
      841 gactcgacca gttcggcgga ggggggttcg gttccgtcca gctcgtagct cataccgatc
      901 tcggcccact tgtcgcgacc catctggtgg aagggcagca cctcgacgcg gctcacacag
      961 cccagggacg ccgcgtagtc ggcgatcggc tcgatggtgg ccgggttgtc ggtcaggcct
     1021 ggcacgagca cgaagcggat ccaggtctcg atgccgaggg tgtccaggcg gcgtccgaaa
     1081 tcgagggtgg gttgcaggcg gcgtccggtg acccggtggt aggtcggctc gtcgccggcc
     1141 ttcacgtcga gcagcaccag gtcgacgtcg gcgagcagct cgtcgtcggc gttgcggccc
     1201 agaaagccgg aggtgtcgat cgcggtgtgg atgccgagcc gcttcgccgc gcgcagaatg
     1261 cggccggcga acgcgggctg ctgcagcact tcgccccccg acagcgtgat gccgccgccg
     1321 gtggccctga acacaccccg gtagcgcgtg atccgggcca tcaggtcgtc cgccagcacc
     1381 ggcacgccgc ggttggcggc catcgtgtcg gggttgtggc agtacaggca gcgcagtggg
     1441 cagccggcca ggaaggtggt catccgggtg ccgggcccgt ccaccgcggt gaccagttcc
     1501 cagctgtgca cggatccgac ctcacccgtc cgctgggcca gcagaagttc gctgcggctc
     1561 aggtgatcgg ccaccggaat gccgccgagc atggtggccc ggacgggcgg gggcggggtg
     1621 agtccggacc caggaaggcc gggggttggc agggtctcgc ccggggcgac ggaccggccc
     1681 gggatgacgg accggcccgg ctcgtccagc ggcccggcct cggccacagc gaaggtcata
     1741 ccgactcgtg gaaggtgcgc gacagcacgt ccagctgctg ctcacgggtg agcttgacga
     1801 agttcacggc gtaacccgag acccggatgg tgagttgcgg gtagttctcg gggtactcca
     1861 tcgcatcgag cagggtttcc ttgttcagca cgttgatgtt cgcgtggtag agaccctcgc
     1921 ccatgccggc gtccagaatg ccgaccaggt tcgccacccg ctcgctctcg gtgcgaccca
     1981 gcgccggcgg ggtgatcgtg ttggtcagcg agatgccgtc cagggcgtcg tcgtagctga
     2041 gcttgccgac gctcatcatg gaggccagca tgccgtgggt gtcggcgccg ttctcggggt
     2101 tggcgcccgg cgcgaacggg gtgccggcct cgtgaccgca gggaaacgcg ccggtcgcct
     2161 tgccgtacac cacgttgctg gtgatggtca gcaccgactg ggtcggaatc gcgtcgcgat
     2221 acagcgggat gtgccggatc ttgtccatca ccgtcgacac caccagtgtg gcgatctcgt
     2281 cggcccggtc atcgtcgttg ccgtagcggg ggaactcgcc ctcggtgacg tagtcgacca
     2341 ccaggccgga ttcgtcacgc accggggtga ccttggcgta cttgatcgca gccagcgagt
     2401 cggccacgat cgacagcccg gcgatgccgc agcccatggt gcgcacgatc tcgtcgtcgt
     2461 gcaacgccat ctccatggcc tcgtaggcgt acttgtcgtg gctgta
//
LOCUS       DAOSUG010000472         2495 bp    DNA     linear   ENV 10-JUN-2024
DEFINITION  MAG TPA_asm: Micropruina sp. isolate AMR_MDS_2195
            NODE_16909_length_2495_cov_3.695902, whole genome shotgun sequence.
ACCESSION   DAOSUG010000472 DAOSUG010000000
VERSION     DAOSUG010000472.1
DBLINK      BioProject: PRJNA925477
            BioSample: SAMN32864262
            Sequence Read Archive: SRR4236673
KEYWORDS    WGS; ENV; Metagenome Assembled Genome; MAG; Third Party Data; TPA;
            TPA:assembly.
SOURCE      Micropruina sp. (wastewater metagenome)
  ORGANISM  Micropruina sp.
            Bacteria; Bacillati; Actinomycetota; Actinomycetes;
            Propionibacteriales; Nocardioidaceae; Micropruina.
REFERENCE   1  (bases 1 to 2495)
  AUTHORS   Abdulkadir,N., Saraiva,J.P., Zhang,J., Stolte,S., Gillor,O.,
            Harms,H. and Rocha,U.
  TITLE     Genome-centric analyses of 165 metagenomes show that mobile genetic
            elements are crucial for the transmission of antimicrobial
            resistance genes to pathogens in activated sludge and wastewater
  JOURNAL   Microbiol Spectr 12 (3), e0291823 (2024)
   PUBMED   38289113
REFERENCE   2  (bases 1 to 2495)
  AUTHORS   Abdulkadir,N.
  TITLE     Direct Submission
  JOURNAL   Submitted (24-MAR-2023) Environmental Microbiology, Helmholtz
            Center for Environmental Research -UFZ, Permoserstr 15, Leipzig
            04318, Germany
COMMENT     The annotation was added by the NCBI Prokaryotic Genome Annotation
            Pipeline (PGAP). Information about PGAP can be found here:
            https://www.ncbi.nlm.nih.gov/genome/annotation_prok/
            
            ##Genome-Assembly-Data-START##
            Assembly Method        :: SPAdes v. 3.11.1
            Genome Representation  :: Full
            Expected Final Version :: Yes
            Genome Coverage        :: Not Applicable
            Sequencing Technology  :: Illumina MiSeq
            ##Genome-Assembly-Data-END##
            
            ##Genome-Annotation-Data-START##
            Annotation Provider               :: NCBI
            Annotation Date                   :: 05/04/2023 04:57:10
            Annotation Pipeline               :: NCBI Prokaryotic Genome
                                                 Annotation Pipeline (PGAP)
            Annotation Method                 :: Best-placed reference protein
                                                 set; GeneMarkS-2+
            Annotation Software revision      :: 6.5
            Features Annotated                :: Gene; CDS; rRNA; tRNA; ncRNA
            Genes (total)                     :: 2,776
            CDSs (total)                      :: 2,750
            Genes (coding)                    :: 2,714
            CDSs (with protein)               :: 2,714
            Genes (RNA)                       :: 26
            tRNAs                             :: 24
            ncRNAs                            :: 2
            Pseudo Genes (total)              :: 36
            CDSs (without protein)            :: 36
            Pseudo Genes (ambiguous residues) :: 0 of 36
            Pseudo Genes (frameshifted)       :: 2 of 36
            Pseudo Genes (incomplete)         :: 32 of 36
            Pseudo Genes (internal stop)      :: 2 of 36
            ##Genome-Annotation-Data-END##
FEATURES             Location/Qualifiers
     source          1..2495
                     /organism="Micropruina sp."
                     /mol_type="genomic DNA"
                     /submitter_seqid="NODE_16909_length_2495_cov_3.695902"
                     /isolate="AMR_MDS_2195"
                     /isolation_source="Activated sludge of municipal
                     wastewater treatment plants"
                     /db_xref="taxon:2737536"
                     /environmental_sample
                     /geo_loc_name="Canada"
                     /lat_lon="49.25 N 123.1 W"
                     /collection_date="2015-06-16"
                     /metagenome_source="wastewater metagenome"
                     /note="metagenomic"
     gene            <1..1017
                     /gene="sufD"
                     /locus_tag="PLF56_12860"
     CDS             <1..1017
                     /gene="sufD"
                     /locus_tag="PLF56_12860"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_011755995.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_function: GO:0005198 - structural molecule activity
                     [Evidence IEA];
                     GO_process: GO:0016226 - iron-sulfur cluster assembly
                     [Evidence IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="Fe-S cluster assembly protein SufD"
                     /protein_id="HOQ54487.1"
                     /translation="MRPLLTDEPSDAHLQWTESFPEGVTTHVVTPTEAKLLVAEPPID
                     RVSALAAARCGGAKVIEVAAGAELTEPVSITLTGTGEHVWGHVIFKVGANSTSTILLR
                     HEGSASYATKVDVLVGDGARVNFVSIQDWADDALHGGHHSVLVGRDAQVRTVTVSLGG
                     KVVRLAETAKFAGPGGSLEQFGLYFVDAGQHIEHRLFVDHNAPHTHSNVDYRGALQGE
                     GARSVWIGDVLIRKVAEGISTFESNKNLVLTDGCRADSVPNLEIETGEIEGAGHSSTT
                     GRFDDEQLFYLRSRGISEAEARRLVVRGFFADIIRRIGVPEVEERMLTAVERELSVTM
                     GAYA"
     gene            1014..1346
                     /locus_tag="PLF56_12865"
     CDS             1014..1346
                     /locus_tag="PLF56_12865"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_016670195.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="non-heme iron oxygenase ferredoxin subunit"
                     /protein_id="HOQ54488.1"
                     /translation="MTFRHAATLAEIPLGTSIPVDLGGDAEVAVVNTAEGVFAIRDVC
                     SHGEVPLSEGEVEGCMIECYMHGSRFDLRTGEPLELPATQPVPVYPVRLDGSDIYVDI
                     DNPINQEN"
     gene            1352..2116
                     /gene="sufC"
                     /locus_tag="PLF56_12870"
     CDS             1352..2116
                     /gene="sufC"
                     /locus_tag="PLF56_12870"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_020573194.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_function: GO:0005524 - ATP binding [Evidence IEA];
                     GO_process: GO:0016226 - iron-sulfur cluster assembly
                     [Evidence IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="Fe-S cluster assembly ATPase SufC"
                     /protein_id="HOQ54489.1"
                     /translation="MSTLTITDLRVEVATETGPKEILKGVNLTVRSGEVHAIMGPNGS
                     GKSTLAYAIAGHPKYTVTGGSVTLDGVELTQLTVDERARAGLFLAMQYPTEVPGVSVA
                     NFLRTAKTALSGEAPKVRTWVKDVNAALDRLGLDPTFSARSLNEGFSGGEKKRNEIVQ
                     LELLNPKFAILDETDSGLDIDALRIVSEGVNRFAAEGDRGVLLITHYTRILNYIKPDF
                     VHVFVDGRVAEEGGPELAENLEIEGYERFIKAAAAS"
     gene            2154..>2495
                     /locus_tag="PLF56_12875"
     CDS             2154..>2495
                     /locus_tag="PLF56_12875"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_002524793.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="aminotransferase class V-fold PLP-dependent
                     enzyme"
                     /protein_id="HOQ54490.1"
                     /translation="MSYDVELIRSDFPILSRRVGEYPLAYLDSANTSQKPQVVIDAMV
                     DHYERHNANVARAMHVLGAEASAAYEAGRDKVAGFIGANRSDEVVFTKNASEALNLAD
                     HTLGATLQPGDE"
BASE COUNT          424 a          859 c          843 g          369 t
ORIGIN      
        1 atgcgtccgc tgctgaccga cgagcccagc gatgcccacc tgcagtggac cgagagcttt
       61 cccgagggcg tgaccaccca cgtggtgacc cccaccgagg ccaagctgct ggtcgccgag
      121 ccgccgatcg accgggtctc cgcactggcg gcggcccgtt gcggcggtgc gaaggtgatc
      181 gaggtggcgg ccggcgccga actcaccgag ccggtgagca tcacgctgac cggcaccggc
      241 gagcacgttt ggggtcacgt gatcttcaag gtcggggcca actcgacctc gacgatcctg
      301 ctgcgccacg agggctcggc cagctacgcc accaaggtgg acgtgctggt cggcgacggt
      361 gcccgggtca acttcgtctc gatccaggac tgggccgacg acgcgctgca cggcgggcac
      421 cacagtgtgc tggtggggcg tgacgcccag gtgcgcacgg tcacggtcag cctgggcggc
      481 aaggtcgtcc ggctcgccga gaccgccaag ttcgccggcc ccggcggttc gctggagcag
      541 ttcggcctgt acttcgtgga cgccggccag cacatcgagc atcggctgtt cgtcgaccac
      601 aacgccccgc acacccacag caacgtcgac taccgcggcg cgctgcaggg cgagggggcg
      661 cgttcggtgt ggatcggcga cgtgctgatc cgcaaggtcg ccgagggcat ctcgaccttc
      721 gagtcgaaca agaacctggt gctcaccgac ggctgccggg ccgactcggt accgaacctc
      781 gaaatcgaga ccggcgagat cgagggggcc gggcacagct cgaccaccgg acgtttcgac
      841 gacgagcagc tgttctacct gcgttcgcgc ggcatcagcg aggccgaggc ccgccgtctg
      901 gtggtgcgcg gcttcttcgc cgacatcatc cggcggatcg gcgtgcccga ggtcgaagag
      961 cgcatgctga cggccgtgga gcgcgagctc tcggtcacca tgggcgccta cgcatgacgt
     1021 ttcggcatgc cgcgacgttg gcggaaattc cgctcggcac gtccattccg gtcgacctcg
     1081 gcggtgacgc cgaggtggcc gtggtgaaca cagccgaggg ggttttcgcg atccgtgacg
     1141 tctgcagcca cggtgaggtg ccgctcagcg agggcgaggt ggagggctgc atgatcgagt
     1201 gctacatgca cggctcccgc ttcgacctgc gcaccggcga acccctcgaa ctacccgcca
     1261 cccagcccgt gccggtctac ccggtgcggc tggacggctc cgacatctac gtcgacatcg
     1321 acaacccgat caaccaggag aactgaaccg catgtccact ttgaccatca ccgacctgcg
     1381 ggtcgaggtc gccaccgaga ccggtccgaa agagatcctc aagggcgtca acctcacggt
     1441 gcgctccggt gaggtgcacg cgatcatggg ccccaacggc tccggcaagt cgacgctggc
     1501 ctacgccatc gccggtcacc ccaagtacac cgtcaccggc ggctcggtca ccctcgacgg
     1561 cgtcgagctg acccagctga ccgtggacga gcgcgcgcgg gccggactgt tcctggccat
     1621 gcagtacccg accgaggtgc ccggcgtgtc cgtggccaac tttctgcgca ccgccaagac
     1681 cgcactgagc ggtgaggcgc ccaaggtgcg cacctgggtc aaggacgtca acgccgccct
     1741 cgaccggctc ggactcgacc cgaccttctc ggcccgctcg ctcaacgagg gtttctcggg
     1801 cggcgagaag aagcgcaacg agatcgtcca gctcgaactg ctcaacccca agttcgcgat
     1861 tctggacgag accgactcgg gcctcgacat cgacgccctg cggatcgtct ccgaaggggt
     1921 caaccggttc gccgccgagg gcgatcgcgg ggtgctgctg atcacccact acacgcgcat
     1981 cctgaactac atcaagcccg acttcgtcca cgtgttcgtg gacgggcggg tggccgaaga
     2041 gggcggcccc gaactggccg agaacctcga gatcgagggc tacgagcggt tcatcaaggc
     2101 cgccgccgcc agctgagccg ggtggcgcca gccgtccatc tgatcggaga gccgtgagct
     2161 acgacgtcga actgattcgc tcggacttcc ccattctgag ccgtcgggtg ggggagtatc
     2221 cgctggcgta cctcgattcg gccaacacct cgcagaagcc gcaggtggtg atcgacgcga
     2281 tggtcgacca ctacgagcgg cacaacgcca acgtggcgcg cgcgatgcac gtgctggggg
     2341 ccgaggcgtc cgccgcctac gaggccggcc gcgacaaggt ggcgggcttc atcggcgcga
     2401 accggtccga cgaggtggtg ttcaccaaga acgcgtccga ggcgctcaac ctggccgacc
     2461 acaccctggg ggccacgctg cagccgggcg acgag
//
LOCUS       DAOSUG010000473         2493 bp    DNA     linear   ENV 10-JUN-2024
DEFINITION  MAG TPA_asm: Micropruina sp. isolate AMR_MDS_2195
            NODE_16926_length_2493_cov_4.162428, whole genome shotgun sequence.
ACCESSION   DAOSUG010000473 DAOSUG010000000
VERSION     DAOSUG010000473.1
DBLINK      BioProject: PRJNA925477
            BioSample: SAMN32864262
            Sequence Read Archive: SRR4236673
KEYWORDS    WGS; ENV; Metagenome Assembled Genome; MAG; Third Party Data; TPA;
            TPA:assembly.
SOURCE      Micropruina sp. (wastewater metagenome)
  ORGANISM  Micropruina sp.
            Bacteria; Bacillati; Actinomycetota; Actinomycetes;
            Propionibacteriales; Nocardioidaceae; Micropruina.
REFERENCE   1  (bases 1 to 2493)
  AUTHORS   Abdulkadir,N., Saraiva,J.P., Zhang,J., Stolte,S., Gillor,O.,
            Harms,H. and Rocha,U.
  TITLE     Genome-centric analyses of 165 metagenomes show that mobile genetic
            elements are crucial for the transmission of antimicrobial
            resistance genes to pathogens in activated sludge and wastewater
  JOURNAL   Microbiol Spectr 12 (3), e0291823 (2024)
   PUBMED   38289113
REFERENCE   2  (bases 1 to 2493)
  AUTHORS   Abdulkadir,N.
  TITLE     Direct Submission
  JOURNAL   Submitted (24-MAR-2023) Environmental Microbiology, Helmholtz
            Center for Environmental Research -UFZ, Permoserstr 15, Leipzig
            04318, Germany
COMMENT     The annotation was added by the NCBI Prokaryotic Genome Annotation
            Pipeline (PGAP). Information about PGAP can be found here:
            https://www.ncbi.nlm.nih.gov/genome/annotation_prok/
            
            ##Genome-Assembly-Data-START##
            Assembly Method        :: SPAdes v. 3.11.1
            Genome Representation  :: Full
            Expected Final Version :: Yes
            Genome Coverage        :: Not Applicable
            Sequencing Technology  :: Illumina MiSeq
            ##Genome-Assembly-Data-END##
            
            ##Genome-Annotation-Data-START##
            Annotation Provider               :: NCBI
            Annotation Date                   :: 05/04/2023 04:57:10
            Annotation Pipeline               :: NCBI Prokaryotic Genome
                                                 Annotation Pipeline (PGAP)
            Annotation Method                 :: Best-placed reference protein
                                                 set; GeneMarkS-2+
            Annotation Software revision      :: 6.5
            Features Annotated                :: Gene; CDS; rRNA; tRNA; ncRNA
            Genes (total)                     :: 2,776
            CDSs (total)                      :: 2,750
            Genes (coding)                    :: 2,714
            CDSs (with protein)               :: 2,714
            Genes (RNA)                       :: 26
            tRNAs                             :: 24
            ncRNAs                            :: 2
            Pseudo Genes (total)              :: 36
            CDSs (without protein)            :: 36
            Pseudo Genes (ambiguous residues) :: 0 of 36
            Pseudo Genes (frameshifted)       :: 2 of 36
            Pseudo Genes (incomplete)         :: 32 of 36
            Pseudo Genes (internal stop)      :: 2 of 36
            ##Genome-Annotation-Data-END##
FEATURES             Location/Qualifiers
     source          1..2493
                     /organism="Micropruina sp."
                     /mol_type="genomic DNA"
                     /submitter_seqid="NODE_16926_length_2493_cov_4.162428"
                     /isolate="AMR_MDS_2195"
                     /isolation_source="Activated sludge of municipal
                     wastewater treatment plants"
                     /db_xref="taxon:2737536"
                     /environmental_sample
                     /geo_loc_name="Canada"
                     /lat_lon="49.25 N 123.1 W"
                     /collection_date="2015-06-16"
                     /metagenome_source="wastewater metagenome"
                     /note="metagenomic"
     gene            complement(153..1580)
                     /locus_tag="PLF56_12880"
     CDS             complement(153..1580)
                     /locus_tag="PLF56_12880"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_012920220.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_function: GO:0003723 - RNA binding [Evidence IEA];
                     GO_function: GO:0004540 - ribonuclease activity [Evidence
                     IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="RNB domain-containing ribonuclease"
                     /protein_id="HOQ54491.1"
                     /translation="MPSRHVAFLDEVPPPLREGLRQLRDALGVPEDFPPEVVEAAEAA
                     VADVRLPEQDLTDVEFITIDPEGSMDLDQALHLSRTDGGYLVRYAIADVAAFVEPGGA
                     IDDECHERVETLYAPNRRTPLHPEVLSEAAASLLPDQTRPALVWQIHLDADGAPTDAS
                     VARARVRSRRRYSYEQVQAAVDAGTGGELLDLLKVVGQLREQAEIARGGVSLGVPEQE
                     VRVADGTWTLEYRTNLPCEGWNAQLSLLTGMVAARMMLEAGVGIVRTLPPAEDHAINR
                     LRRTALALGISWPQRQSYPDFVRGLKASVPREAAMLNACTTLFRGAGYDSFTDGAPSG
                     AEHAAIAAPYAHVTAPLRRLADRYAGEICVALSAGQPVPQWVLDALDALPSEMQDSAR
                     RAGNYERGIIDLVEAMVLAPRIGQRFPAVLIDVDPDRKAGRLQVQQPAVEARVKGKRM
                     KLGSEVTATLVAADLVKGSVDFRVF"
     gene            complement(1660..>2493)
                     /locus_tag="PLF56_12885"
     CDS             complement(1660..>2493)
                     /locus_tag="PLF56_12885"
                     /inference="COORDINATES: protein motif:HMM:NF021588.2"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="alpha/beta-hydrolase family protein"
                     /protein_id="HOQ54492.1"
                     /translation="GDCATISIQYSTLASAFVFLADRETPRESAFALLAAIEDEIELL
                     PHDRRPAIYVSGESLGSYGGHGAFIDADDMIERVAGAVWIGTPGFTRIARTLTQSRMR
                     GSPEITPVIDNGRHIRFVTKPTELVADAYGRVLGPWQAPRIVYVAHASDPMARFTADL
                     AWREPDWMRERAGSDVNASLRWWPLVTWFQTSTDMATSLDTPPGHGHIYEDELVPVWA
                     AVLDRPDAPVTAITAAIRASVDKLKKPRPGAVSSSSGPGSGSRGSLSTGGGLGGPGAA
                     E"
BASE COUNT          376 a          906 c          859 g          352 t
ORIGIN      
        1 tcacccaccc gtcgggccgg acagctcaag cacaagacac cccgcaatgc gagggtcagt
       61 cgagaccaga gtcacggccc gacgagcacc acgacactaa acgtcgagac ccggacaagg
      121 atctcgacaa gctcgatcaa cgggacccgc tttcagaaga cccggaagtc caccgagccc
      181 ttcaccagat cggcagccac cagggtggcg gtcacctcgc tgcccagctt catccgcttg
      241 cccttcaccc gtgcctcgac ggccggctgc tgcacctgca gccggcccgc ctttcggtct
      301 gggtcgacgt cgatcagcac ggctggaaag cgctgcccga tgcgcggcgc cagcaccatg
      361 gcttcgacca ggtcgatgat gccccgctcg tagttgccgg cccggcgcgc cgagtcctgc
      421 atctcgctgg gcagggcgtc cagtgcgtcg agcacccact gcggcaccgg ctgccccgcg
      481 ctgagcgcca cgcagatctc accggcgtag cggtccgcca gccggcgcag cggcgccgtc
      541 acatgggcgt agggcgccgc gatggccgcg tgttcggcgc cactgggggc gccatcggtg
      601 aacgagtcgt accctgctcc gcgaaacagc gtggtgcagg cgttgagcat ggccgcctcg
      661 cgcggcaccg atgccttcag ccctcggacg aagtcgggat acgactgccg ctgcggccac
      721 gagataccga gcgccaaagc ggtgcggcgc agccgattga tcgcatggtc ctccgccggc
      781 ggcagagtgc gcacaatgcc cacgcccgcc tccagcatca tgcgggcagc caccatgccg
      841 gtgagcaacg acagctgggc gttccacccc tcgcagggca ggttggtgcg gtattccagg
      901 gtccaggtgc cgtcggcgac cctgacctcc tgctcgggca cgcccaggct gacgccgccg
      961 cgcgcgatct cggcctgttc gcgcagctgc ccgaccacct tgagcaggtc gagcagttcg
     1021 ccgcccgtgc ccgcgtcgac ggccgcctga acctgctcgt agctgtagcg ccgccggctg
     1081 cgcacccggg ctcgggccac cgacgcgtcg gtgggcgcgc cgtcggcgtc caggtgaatc
     1141 tgccacacca gcgccggacg ggtctgatcg ggcagcaggc tcgccgccgc ctcgctcagc
     1201 acctcagggt gcaggggcgt gcgccggttg ggcgcgtaca acgtctcgac tcgctcgtgg
     1261 cactcgtcat cgatcgctcc cccgggttcg acgaaggcag ccacgtcggc gatggcgtag
     1321 cgcaccaggt agccgccgtc ggtgcgcgac aggtgcaggg cctggtcgag gtccatcgac
     1381 ccctcggggt cgatggtgat gaactccacg tcggtgaggt cctgctccgg cagccggacg
     1441 tccgccaccg cggcctcggc cgcctcgacc acctccggcg gaaagtcctc cggcacgccg
     1501 agcgcgtcgc gcaactggcg cagcccttcg cgaagcggcg gcggaacctc gtcgagaaag
     1561 gcgacatgac ggctgggcat cgatgacctc cggatcggtt gcgttcaccc taaccggacg
     1621 cgcctcggtc gggcggaggc gaggccccgg cgcaccggcc tactccgcgg cgcccggccc
     1681 gcccaggccg ccgccggtgc tcagcgatcc acgcgacccc gaccccgggc cggacgaaga
     1741 cgacaccgca ccgggccgcg gcttcttcag cttgtcgaca gaggccctga tggccgccgt
     1801 gatcgcggtg accggagcgt ccgggcggtc gagcacggcc gcccacaccg gcaccagttc
     1861 gtcctcgtag atgtgtccgt ggccgggtgg ggtgtcgagg ctggtggcca tgtcggtgct
     1921 ggtctgaaac caggtgacca gcggccacca gcgcagcgag gcgttgacgt cgctgccggc
     1981 ccgctcgcgc atccagtccg gctcgcgcca ggccaggtcg gcggtgaacc gggccatcgg
     2041 gtccgaggcg tgcgccacgt aaacgatgcg gggagcctgc cagggaccga gcacccgccc
     2101 gtaggcatcg gcgacgagtt cggtgggctt ggtgacgaag cggatgtgcc ggccgttgtc
     2161 gatcaccggc gtgatctcgg gcgagcctcg catgcgcgac tgggtgagcg tgcgggcgat
     2221 cctggtgaag ccgggggtgc cgatccacac cgcgccggcg acccgctcga tcatgtcgtc
     2281 ggcatcgatg aacgccccgt gcccgccgta gctgcccagc gactcgccgc tgacatagat
     2341 ggccggacgg cggtcgtgcg gcaacagctc gatctcgtcc tcgatcgcgg ccagcagggc
     2401 gaacgcgctt tcgcgcgggg tctcgcggtc ggccaggaac acgaatgcac tggccagcgt
     2461 cgagtactga atcgagatcg tcgcgcagtc gcc
//
LOCUS       DAOSUG010000474         2485 bp    DNA     linear   ENV 10-JUN-2024
DEFINITION  MAG TPA_asm: Micropruina sp. isolate AMR_MDS_2195
            NODE_17024_length_2485_cov_3.341152, whole genome shotgun sequence.
ACCESSION   DAOSUG010000474 DAOSUG010000000
VERSION     DAOSUG010000474.1
DBLINK      BioProject: PRJNA925477
            BioSample: SAMN32864262
            Sequence Read Archive: SRR4236673
KEYWORDS    WGS; ENV; Metagenome Assembled Genome; MAG; Third Party Data; TPA;
            TPA:assembly.
SOURCE      Micropruina sp. (wastewater metagenome)
  ORGANISM  Micropruina sp.
            Bacteria; Bacillati; Actinomycetota; Actinomycetes;
            Propionibacteriales; Nocardioidaceae; Micropruina.
REFERENCE   1  (bases 1 to 2485)
  AUTHORS   Abdulkadir,N., Saraiva,J.P., Zhang,J., Stolte,S., Gillor,O.,
            Harms,H. and Rocha,U.
  TITLE     Genome-centric analyses of 165 metagenomes show that mobile genetic
            elements are crucial for the transmission of antimicrobial
            resistance genes to pathogens in activated sludge and wastewater
  JOURNAL   Microbiol Spectr 12 (3), e0291823 (2024)
   PUBMED   38289113
REFERENCE   2  (bases 1 to 2485)
  AUTHORS   Abdulkadir,N.
  TITLE     Direct Submission
  JOURNAL   Submitted (24-MAR-2023) Environmental Microbiology, Helmholtz
            Center for Environmental Research -UFZ, Permoserstr 15, Leipzig
            04318, Germany
COMMENT     The annotation was added by the NCBI Prokaryotic Genome Annotation
            Pipeline (PGAP). Information about PGAP can be found here:
            https://www.ncbi.nlm.nih.gov/genome/annotation_prok/
            
            ##Genome-Assembly-Data-START##
            Assembly Method        :: SPAdes v. 3.11.1
            Genome Representation  :: Full
            Expected Final Version :: Yes
            Genome Coverage        :: Not Applicable
            Sequencing Technology  :: Illumina MiSeq
            ##Genome-Assembly-Data-END##
            
            ##Genome-Annotation-Data-START##
            Annotation Provider               :: NCBI
            Annotation Date                   :: 05/04/2023 04:57:10
            Annotation Pipeline               :: NCBI Prokaryotic Genome
                                                 Annotation Pipeline (PGAP)
            Annotation Method                 :: Best-placed reference protein
                                                 set; GeneMarkS-2+
            Annotation Software revision      :: 6.5
            Features Annotated                :: Gene; CDS; rRNA; tRNA; ncRNA
            Genes (total)                     :: 2,776
            CDSs (total)                      :: 2,750
            Genes (coding)                    :: 2,714
            CDSs (with protein)               :: 2,714
            Genes (RNA)                       :: 26
            tRNAs                             :: 24
            ncRNAs                            :: 2
            Pseudo Genes (total)              :: 36
            CDSs (without protein)            :: 36
            Pseudo Genes (ambiguous residues) :: 0 of 36
            Pseudo Genes (frameshifted)       :: 2 of 36
            Pseudo Genes (incomplete)         :: 32 of 36
            Pseudo Genes (internal stop)      :: 2 of 36
            ##Genome-Annotation-Data-END##
FEATURES             Location/Qualifiers
     source          1..2485
                     /organism="Micropruina sp."
                     /mol_type="genomic DNA"
                     /submitter_seqid="NODE_17024_length_2485_cov_3.341152"
                     /isolate="AMR_MDS_2195"
                     /isolation_source="Activated sludge of municipal
                     wastewater treatment plants"
                     /db_xref="taxon:2737536"
                     /environmental_sample
                     /geo_loc_name="Canada"
                     /lat_lon="49.25 N 123.1 W"
                     /collection_date="2015-06-16"
                     /metagenome_source="wastewater metagenome"
                     /note="metagenomic"
     gene            complement(<1..428)
                     /locus_tag="PLF56_12890"
     CDS             complement(<1..428)
                     /locus_tag="PLF56_12890"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_011753835.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="endonuclease III"
                     /protein_id="HOQ54493.1"
                     /translation="METRTALVRRARAINRALALTYPDATCELDFRNPLELLVATILS
                     AQSTDKRVNLVTPVLFARYPSAADYAAADREALEDLIRPTGFFRAKTDSLIKLGAALG
                     QRFGGEVPGRLTDLVSLPGVGRKTANVVLGNAFGVPGITT"
     gene            complement(428..919)
                     /locus_tag="PLF56_12895"
     CDS             complement(428..919)
                     /locus_tag="PLF56_12895"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_011753831.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="RidA family protein"
                     /protein_id="HOQ54494.1"
                     /translation="MNEEVDGPAPSARLAELGLSLPAVAKPLAAYIPAIASAGQVFTS
                     GQLPTVEGVLVATGKVGAEVSLEQAVEAARVAALNALAAAASAVGGIDEIGRIVRVVV
                     YVASDPTFTGQPLVANGASELFGSIFGALGVHVRSAVGVSCLPLDAPVELELVVEASA
                     ASF"
     gene            complement(916..1077)
                     /locus_tag="PLF56_12900"
     CDS             complement(916..1077)
                     /locus_tag="PLF56_12900"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_020391043.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="DUF4177 domain-containing protein"
                     /protein_id="HOQ54495.1"
                     /translation="MTKWEYVTVPVLVHVTKQILDQWGADGWELVQVVPGPNPQNLVA
                     YLKRPLSDS"
     gene            1149..2039
                     /locus_tag="PLF56_12905"
     CDS             1149..2039
                     /locus_tag="PLF56_12905"
                     /inference="COORDINATES: protein motif:HMM:NF014433.2"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="ArsA-related P-loop ATPase"
                     /protein_id="HOQ54496.1"
                     /translation="MLARCPLHIVTGKGGVGKTTIALALALALARQGARVVVAEVEGR
                     GGLALAAGRPVSSTPTRLLRTRSGLIEGVQIEAKASLSGYLQRFYHLGFAADLLQLDP
                     LVDFATRIAPGLRDILLIGQVTHLTKTHPGIDALVLDAPPSGRIGSFLGAGEGLAELT
                     DGSPIAAQAASVMDDLRDPGCRTHVVTLLDELPVSETIEIVTVVRDLNLRLGTVVANR
                     CIAAPVVPTPLPPAPAPLSHDQWAAMTTALLAEADQAAGQNAWEALLRHRTGLSVTRV
                     PDTDPSGHGLSLLADHLEGV"
     gene            2036..>2485
                     /locus_tag="PLF56_12910"
     CDS             2036..>2485
                     /locus_tag="PLF56_12910"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_017933307.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="ArsA-related P-loop ATPase"
                     /protein_id="HOQ54497.1"
                     /translation="MNTLDLDRLLLQPQVRLLLCVGTGGVGKTTLSAALGLRAAELGR
                     RVVVVTIDPARRLAQAMGLDGLVHEPAPVPLPGATGTLDAMMLDVRHAFDQALTAQLP
                     ADEADRIRSNPFYKALSEAFSGTQEYVAMEKIGELSRQAAATGRWDLI"
BASE COUNT          383 a          928 c          814 g          360 t
ORIGIN      
        1 tcggtggtga tgccgggcac gccgaacgcg ttgccgagca ccacgttggc ggtcttgcgg
       61 cccacgcccg gcaggctcac caggtcggtc agtcgacccg gcacctcgcc accgaagcgc
      121 tgccccagag ctgcccccag cttgatcagc gagtcggtct tggcgcgaaa gaacccggtg
      181 ggccggatca gatcttcgag cgcctcacga tcggccgccg catagtcggc cgccgacggg
      241 tagcgcgcga aaagaaccgg cgtgaccagg ttgacccgct tgtcggtcga ctgggcgctc
      301 agaatcgtcg ccaccaacaa ctcgagcggg ttgcgaaagt cgagttcgca ggtggcgtcg
      361 ggataggtca gcgccagggc acggttgatc gcccgggcgc gccggacgag cgccgtgcga
      421 gtctccatca gaacgacgcg gcgctggctt caaccaccag ctcgagctcg accggagcgt
      481 ccaagggcag gcaactgacc ccgaccgccg agcgcacatg caccccgagc gcaccgaaga
      541 tcgaaccgaa cagttcgctg gcaccgttgg ccaccagcgg ctgaccggtg aaggtggggt
      601 cgctcgccac gtagacgacc acgcgcacga tgcggccgat ctcatcgatg ccccccaccg
      661 ccgatgcggc cgcggcgagc gcgttcagcg ccgccactcg ggcggcctcg accgcctgct
      721 cgaggctcac ctcagcgccg accttgccgg tggcgaccag caccccctca accgtcggca
      781 actgaccgga ggtgaacacc tgcccagccg acgcgatcgc cggaatgtag gcggccagcg
      841 gcttggccac cgccggaagg ctcagcccga gttcggccag ccgggccgaa ggcgccggcc
      901 catccacttc ttcgttcacg agtcgctcag cggacgcttc aggtaggcga ccaggttctg
      961 cggattgggg cccggtacga cctgcaccag ctcccacccg tcagcgcccc actgatcgag
     1021 aatctgcttg gtcacgtgca ccagcaccgg cacggtcacg tactcccatt tcgtcatgcg
     1081 cccaacccta atggttggca ccgacagcgc cacggggtgc ccgcccaccc cccgcgtacg
     1141 ctggccgcat gctcgcccgc tgcccgctgc acatcgtcac gggtaagggc ggtgtcggca
     1201 agaccacgat cgcgctggcg ctcgccctgg ccctggcgcg ccagggcgca cgcgtggtgg
     1261 tcgccgaggt cgagggtcgc ggcgggctgg ccctggccgc cggacgtccc gtgagcagca
     1321 cccccacccg gctgctgcgc acccgctccg gactgatcga gggcgtgcag atcgaggcca
     1381 aggcctcgct gtcgggctat ctgcaacgct tctaccacct cggcttcgcc gccgacctgc
     1441 tgcaactcga cccgctggtc gactttgcca cccgcatcgc acccggactg cgcgacattc
     1501 tgctgatcgg ccaggtgacg cacctgacga agacccaccc gggcatcgac gccctggtgc
     1561 tggacgcgcc cccgtccggc cggatcggct cgttcctggg cgccggcgag ggcctggccg
     1621 aactgaccga cggcagcccg atcgcggccc aggccgcctc ggtgatggac gacctgcgcg
     1681 atccggggtg ccgcacccat gtggtgaccc tgctcgatga gttgccggtg agcgaaacca
     1741 tcgagatcgt gacggtggtg cgcgacctca atctcaggct gggcacggtg gtggcgaacc
     1801 gttgcatcgc tgcgccggtc gtgccgacgc cgcttccgcc cgcgcccgct ccgctttcgc
     1861 acgatcaatg ggccgccatg acgaccgcgt tgctggccga ggccgatcag gccgccggcc
     1921 agaacgcctg ggaggccctg ctgcggcacc gcaccggcct gagcgtcacg cgcgtgcccg
     1981 atacggaccc gtccggtcac gggctcagcc tgctggccga tcatctggag ggcgtgtgaa
     2041 caccctcgat ctcgatcggc tgctgctgca gccgcaggtg cgcctgctgc tctgcgtcgg
     2101 caccggcggg gtcggcaaga ccacgctgtc cgccgccctc ggcctgcgtg ccgccgagct
     2161 cggccgtcgg gtcgtcgtgg tcaccatcga ccctgcccgg cgactggctc aagccatggg
     2221 cctggacggt ctcgtgcacg aacccgcccc ggtgccactg cccggcgcga ccggaacgct
     2281 cgacgccatg atgctcgacg tacggcacgc cttcgatcag gccctgaccg ctcagctacc
     2341 cgccgacgag gccgaccgca tccgctccaa tcccttctac aaggccttgt cagaggcatt
     2401 ttcgggcacg caggagtacg tggccatgga gaagatcggc gaactgtcgc gccaggccgc
     2461 ggccaccgga cgctgggacc tgatc
//
LOCUS       DAOSUG010000475         2478 bp    DNA     linear   ENV 10-JUN-2024
DEFINITION  MAG TPA_asm: Micropruina sp. isolate AMR_MDS_2195
            NODE_17093_length_2478_cov_2.872472, whole genome shotgun sequence.
ACCESSION   DAOSUG010000475 DAOSUG010000000
VERSION     DAOSUG010000475.1
DBLINK      BioProject: PRJNA925477
            BioSample: SAMN32864262
            Sequence Read Archive: SRR4236673
KEYWORDS    WGS; ENV; Metagenome Assembled Genome; MAG; Third Party Data; TPA;
            TPA:assembly.
SOURCE      Micropruina sp. (wastewater metagenome)
  ORGANISM  Micropruina sp.
            Bacteria; Bacillati; Actinomycetota; Actinomycetes;
            Propionibacteriales; Nocardioidaceae; Micropruina.
REFERENCE   1  (bases 1 to 2478)
  AUTHORS   Abdulkadir,N., Saraiva,J.P., Zhang,J., Stolte,S., Gillor,O.,
            Harms,H. and Rocha,U.
  TITLE     Genome-centric analyses of 165 metagenomes show that mobile genetic
            elements are crucial for the transmission of antimicrobial
            resistance genes to pathogens in activated sludge and wastewater
  JOURNAL   Microbiol Spectr 12 (3), e0291823 (2024)
   PUBMED   38289113
REFERENCE   2  (bases 1 to 2478)
  AUTHORS   Abdulkadir,N.
  TITLE     Direct Submission
  JOURNAL   Submitted (24-MAR-2023) Environmental Microbiology, Helmholtz
            Center for Environmental Research -UFZ, Permoserstr 15, Leipzig
            04318, Germany
COMMENT     The annotation was added by the NCBI Prokaryotic Genome Annotation
            Pipeline (PGAP). Information about PGAP can be found here:
            https://www.ncbi.nlm.nih.gov/genome/annotation_prok/
            
            ##Genome-Assembly-Data-START##
            Assembly Method        :: SPAdes v. 3.11.1
            Genome Representation  :: Full
            Expected Final Version :: Yes
            Genome Coverage        :: Not Applicable
            Sequencing Technology  :: Illumina MiSeq
            ##Genome-Assembly-Data-END##
            
            ##Genome-Annotation-Data-START##
            Annotation Provider               :: NCBI
            Annotation Date                   :: 05/04/2023 04:57:10
            Annotation Pipeline               :: NCBI Prokaryotic Genome
                                                 Annotation Pipeline (PGAP)
            Annotation Method                 :: Best-placed reference protein
                                                 set; GeneMarkS-2+
            Annotation Software revision      :: 6.5
            Features Annotated                :: Gene; CDS; rRNA; tRNA; ncRNA
            Genes (total)                     :: 2,776
            CDSs (total)                      :: 2,750
            Genes (coding)                    :: 2,714
            CDSs (with protein)               :: 2,714
            Genes (RNA)                       :: 26
            tRNAs                             :: 24
            ncRNAs                            :: 2
            Pseudo Genes (total)              :: 36
            CDSs (without protein)            :: 36
            Pseudo Genes (ambiguous residues) :: 0 of 36
            Pseudo Genes (frameshifted)       :: 2 of 36
            Pseudo Genes (incomplete)         :: 32 of 36
            Pseudo Genes (internal stop)      :: 2 of 36
            ##Genome-Annotation-Data-END##
FEATURES             Location/Qualifiers
     source          1..2478
                     /organism="Micropruina sp."
                     /mol_type="genomic DNA"
                     /submitter_seqid="NODE_17093_length_2478_cov_2.872472"
                     /isolate="AMR_MDS_2195"
                     /isolation_source="Activated sludge of municipal
                     wastewater treatment plants"
                     /db_xref="taxon:2737536"
                     /environmental_sample
                     /geo_loc_name="Canada"
                     /lat_lon="49.25 N 123.1 W"
                     /collection_date="2015-06-16"
                     /metagenome_source="wastewater metagenome"
                     /note="metagenomic"
     gene            complement(<1..235)
                     /locus_tag="PLF56_12915"
     CDS             complement(<1..235)
                     /locus_tag="PLF56_12915"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_013861885.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="alcohol dehydrogenase catalytic
                     domain-containing protein"
                     /protein_id="HOQ54498.1"
                     /translation="MFGAVIHAPGDVRYEERADPTIIEPTDAVVRTVATCVCGSDLWR
                     YRGIAPVTRPTPIGHEFCGVVEQVGASVTSVKPG"
     gene            complement(273..>567)
                     /locus_tag="PLF56_12920"
     CDS             complement(273..>567)
                     /locus_tag="PLF56_12920"
                     /inference="COORDINATES: ab initio
                     prediction:GeneMarkS-2+"
                     /note="Derived by automated computational analysis using
                     gene prediction method: GeneMarkS-2+."
                     /codon_start=2
                     /transl_table=11
                     /product="hypothetical protein"
                     /protein_id="HOQ54499.1"
                     /translation="LSDDIALARIEHLWHLYGTKDYLQASGQVMFAGRWPIAKASPWN
                     EALREGRISMIELGPYQHNVKQHYFDYESRLPDGRSYAGKVIDTIEGILEPQR"
     assembly_gap    568..577
                     /estimated_length=10
                     /gap_type="within scaffold"
                     /linkage_evidence="paired-ends"
     gene            complement(<578..1211)
                     /locus_tag="PLF56_12925"
     CDS             complement(<578..1211)
                     /locus_tag="PLF56_12925"
                     /inference="COORDINATES: ab initio
                     prediction:GeneMarkS-2+"
                     /note="Derived by automated computational analysis using
                     gene prediction method: GeneMarkS-2+."
                     /codon_start=1
                     /transl_table=11
                     /product="hypothetical protein"
                     /protein_id="HOQ54500.1"
                     /translation="MIVDLLILVLALLVASLVLAPLESLRWWATQGAEQIEAGGEAAE
                     LRELADTAAADPDAPSEPRKFLVYLSGIGAIGAGDVPQEEVPFVQDLHDRLEEFTVLD
                     DVFPYAVDNRGLTAERFLGRLWRWLEGIRLKNPNAALAVLINLRNLSQLFVCADRRYG
                     PTYNLGTAREIHKSLVRHGHRPGSPDEVVLFGWSGGGQIALGAAWYLQALG"
     gene            complement(1208..1864)
                     /locus_tag="PLF56_12930"
     CDS             complement(1208..1864)
                     /locus_tag="PLF56_12930"
                     /inference="COORDINATES: ab initio
                     prediction:GeneMarkS-2+"
                     /note="Derived by automated computational analysis using
                     gene prediction method: GeneMarkS-2+."
                     /codon_start=1
                     /transl_table=11
                     /product="hypothetical protein"
                     /protein_id="HOQ54501.1"
                     /translation="MDDVGRYLAGLFELITRAYRLDPAALAWVRDQPYSIGLTAGVAL
                     LAGISVMLGHAVVLLLNRITGWRFAIGLLVSAVWLVLLHALESVVLWALGSWASGQPV
                     PYLTVLTGVLVSTAPQIWGFLVLIPYLGTAIGRFLSAWSAVCLWAVTAGTFEVGRWPA
                     LGLVAVAWVVMHLVSLFCSPFISRALSTVFERVAGRPFWVSGADVLNGRPLITNEAQP
                     "
     gene            complement(1912..>2478)
                     /locus_tag="PLF56_12935"
     CDS             complement(1912..>2478)
                     /locus_tag="PLF56_12935"
                     /EC_number="2.7.1.11"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_002519851.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_process: GO:0006096 - glycolytic process [Evidence
                     IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="6-phosphofructokinase"
                     /protein_id="HOQ54502.1"
                     /translation="LGAGIAGGADIILLPEIPYTVESIAETIRERTRRGSTFSVVAVA
                     EGARDVSDTADYEGAQLLVKNAKTPEAQRAARAHLSNVEHSQREHTFTLATQLEQATG
                     LEARVTILGYVQRGGTPSAADRLLATRLGTAAADLVAAGEFGFMVAARGNGTEPVPLK
                     DVAGKVKLVPLDHDWVAAARKVGTGLGD"
BASE COUNT          413 a          857 c          850 g          348 t
ORIGIN      
        1 cgccgggctt caccgaggtg accgacgcgc cgacctgctc gaccacgccg cagaactcgt
       61 ggccgatggg tgtgggcctg gtgaccggcg cgatgccgcg gtagcgccac aaatccgagc
      121 cgcacacgca ggtggcgaca gtgcgtacca ccgcgtcggt gggctcgatg atggtgggat
      181 cggcacgctc ttcgtagcgg acgtccccgg gtgcatgaat cactgctccg aacatgggtc
      241 cacttcatca tggacggcgc ccgcgcggca gttcagcgct gcggttcgag aatgccctcg
      301 atggtgtcga tcaccttgcc ggcgtagctt cgcccgtcgg gcagccggga ctcgtagtcg
      361 aagtagtgct gcttcacgtt gtgctggtag ggacccagtt cgatcatgct gatcctgccc
      421 tccctgagcg cctcgttcca gggtgaggcc ttcgcgatcg gccagcgtcc ggcgaacatc
      481 acctggccgg aggcctgcaa atagtccttg gtgccgtaca ggtgccacag gtgctcgatg
      541 cgggccagcg cgatgtcgtc cgacagcnnn nnnnnnngcc ccaacgcctg caggtaccag
      601 gcggcgccga gggcgatctg accaccaccg ctccagccga aaagcaccac ctcatcgggg
      661 ctgcccggcc ggtggccgtg ccgcaccagc gacttgtgga tctcgcgggc ggtgcccaga
      721 ttgtaggtgg ggccgtagcg acggtcggcg cagacgaaca gctgcgagag gttgcgcagg
      781 ttgatcagca ccgccagggc ggcattgggg ttcttcagcc ggatgccttc gagccagcgc
      841 cacagccggc ccaggaaccg ttcggcggtg agcccccggt tgtcgacggc gtagggaaag
      901 acgtcgtcga gcaccgtgaa ctcttcgagc cggtcatgca gatcctgcac gaacggcacc
      961 tcctcctggg gcacgtcgcc ggcgccgatc gcgccgatgc ccgagaggta gaccaggaac
     1021 ttgcggggct cggacggggc gtcggggtcg gccgccgccg tgtcggccaa ctcgcgcagt
     1081 tcggcggcct cgccgccggc ctcgatctgt tcggcaccct gcgtggccca ccagcgcagt
     1141 gattccagcg gcgccagcac cagcgaggcg accagcaggg cgagcaccag gatcagcagg
     1201 tcgacgatca tggttgcgcc tcgttggtga tcagcggacg accgttcagc acatcagcgc
     1261 ccgagaccca gaacggacgg ccggccaccc gctcgaacac ggtcgacagg gcacgcgaga
     1321 tgaacggcga acagaacaac gacaccaggt gcatcaccac ccaggcgacc gcgaccaacc
     1381 ccagggcggg ccatcgtccg acctcgaagg tgcctgcggt gaccgcccac aggcacaccg
     1441 cgctccaggc cgacaggaac cgtccgatcg cggtgcccag gtaggggatc agcaccagaa
     1501 agccccagat ctgcggcgcc gtcgacacca gcacgccggt cagcacggtc aggtagggca
     1561 ccggttgccc gctcgcccat gagccgagcg cccacaacac caccgattcc aaagcatgca
     1621 gcagcacgag ccacaccgcg ctgaccagca ggccgatggc gaagcgccag ccggtgatcc
     1681 ggttgagcag cagcacgacc gcgtggccca gcatcaccga gatgccggcc agcagcgcca
     1741 cgccggcggt gagcccgatc gagtagggct ggtcgcgcac ccaggccagg gccgccgggt
     1801 cgagccggta ggcacgggtg atcagttcga acagcccggc gaggtagcgc cccacgtcgt
     1861 ccacgcgaga atgctaaccg tcgcgccgtc gcagcgaggc cggcatggac gtcagtcacc
     1921 caggccggtg cccaccttgc gggcggccgc cacccagtcg tgatccaggg gcaccagctt
     1981 caccttgcct gcgacgtcct tgagcgggac gggttcggtg ccgttgccgc gggcggcgac
     2041 catgaagccg aactcaccgg cggcgaccag atcggcggcc gcggtgccca gcctggtggc
     2101 cagcaggcgg tcagcggccg agggggtgcc gccccgctgc acgtagccca gaatggtcac
     2161 cctggcctcg agcccggtgg cctgctccag ttgggtcgcc agggtgaagg tgtgctcgcg
     2221 ttgggagtgc tcgacgttgc tcaggtgggc gcgggcggcc cgctgcgctt cgggcgtctt
     2281 ggcgttcttc accagcaact gggcgccctc gtagtcggcg gtgtcgctca catcacgggc
     2341 accctcggcc acggccacca ccgagaacgt cgagccgcgg cgcgtgcgtt cgcggatcgt
     2401 ctcggcgatg ctctcgaccg tgtagggaat ctcgggcagc agaatgatgt cggcgccgcc
     2461 ggcgataccc gcgcccag
//
LOCUS       DAOSUG010000476         2454 bp    DNA     linear   ENV 10-JUN-2024
DEFINITION  MAG TPA_asm: Micropruina sp. isolate AMR_MDS_2195
            NODE_17325_length_2454_cov_4.525219, whole genome shotgun sequence.
ACCESSION   DAOSUG010000476 DAOSUG010000000
VERSION     DAOSUG010000476.1
DBLINK      BioProject: PRJNA925477
            BioSample: SAMN32864262
            Sequence Read Archive: SRR4236673
KEYWORDS    WGS; ENV; Metagenome Assembled Genome; MAG; Third Party Data; TPA;
            TPA:assembly.
SOURCE      Micropruina sp. (wastewater metagenome)
  ORGANISM  Micropruina sp.
            Bacteria; Bacillati; Actinomycetota; Actinomycetes;
            Propionibacteriales; Nocardioidaceae; Micropruina.
REFERENCE   1  (bases 1 to 2454)
  AUTHORS   Abdulkadir,N., Saraiva,J.P., Zhang,J., Stolte,S., Gillor,O.,
            Harms,H. and Rocha,U.
  TITLE     Genome-centric analyses of 165 metagenomes show that mobile genetic
            elements are crucial for the transmission of antimicrobial
            resistance genes to pathogens in activated sludge and wastewater
  JOURNAL   Microbiol Spectr 12 (3), e0291823 (2024)
   PUBMED   38289113
REFERENCE   2  (bases 1 to 2454)
  AUTHORS   Abdulkadir,N.
  TITLE     Direct Submission
  JOURNAL   Submitted (24-MAR-2023) Environmental Microbiology, Helmholtz
            Center for Environmental Research -UFZ, Permoserstr 15, Leipzig
            04318, Germany
COMMENT     The annotation was added by the NCBI Prokaryotic Genome Annotation
            Pipeline (PGAP). Information about PGAP can be found here:
            https://www.ncbi.nlm.nih.gov/genome/annotation_prok/
            
            ##Genome-Assembly-Data-START##
            Assembly Method        :: SPAdes v. 3.11.1
            Genome Representation  :: Full
            Expected Final Version :: Yes
            Genome Coverage        :: Not Applicable
            Sequencing Technology  :: Illumina MiSeq
            ##Genome-Assembly-Data-END##
            
            ##Genome-Annotation-Data-START##
            Annotation Provider               :: NCBI
            Annotation Date                   :: 05/04/2023 04:57:10
            Annotation Pipeline               :: NCBI Prokaryotic Genome
                                                 Annotation Pipeline (PGAP)
            Annotation Method                 :: Best-placed reference protein
                                                 set; GeneMarkS-2+
            Annotation Software revision      :: 6.5
            Features Annotated                :: Gene; CDS; rRNA; tRNA; ncRNA
            Genes (total)                     :: 2,776
            CDSs (total)                      :: 2,750
            Genes (coding)                    :: 2,714
            CDSs (with protein)               :: 2,714
            Genes (RNA)                       :: 26
            tRNAs                             :: 24
            ncRNAs                            :: 2
            Pseudo Genes (total)              :: 36
            CDSs (without protein)            :: 36
            Pseudo Genes (ambiguous residues) :: 0 of 36
            Pseudo Genes (frameshifted)       :: 2 of 36
            Pseudo Genes (incomplete)         :: 32 of 36
            Pseudo Genes (internal stop)      :: 2 of 36
            ##Genome-Annotation-Data-END##
FEATURES             Location/Qualifiers
     source          1..2454
                     /organism="Micropruina sp."
                     /mol_type="genomic DNA"
                     /submitter_seqid="NODE_17325_length_2454_cov_4.525219"
                     /isolate="AMR_MDS_2195"
                     /isolation_source="Activated sludge of municipal
                     wastewater treatment plants"
                     /db_xref="taxon:2737536"
                     /environmental_sample
                     /geo_loc_name="Canada"
                     /lat_lon="49.25 N 123.1 W"
                     /collection_date="2015-06-16"
                     /metagenome_source="wastewater metagenome"
                     /note="metagenomic"
     gene            complement(<1..2004)
                     /locus_tag="PLF56_12940"
     CDS             complement(<1..2004)
                     /locus_tag="PLF56_12940"
                     /inference="COORDINATES: protein motif:HMM:NF013491.2"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_function: GO:0005524 - ATP binding [Evidence IEA];
                     GO_function: GO:0016301 - kinase activity [Evidence IEA];
                     GO_process: GO:0016310 - phosphorylation [Evidence IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="PEP/pyruvate-binding domain-containing protein"
                     /protein_id="HOQ54503.1"
                     /translation="MLLTERSTVRLTRITANAAAAAGGLAGAWAAGFGPWMLLVAPLT
                     GTLGWFAGFTAGRASARRLARAGYAPSGRPLYRLEHLPPDELSAGGKARSLSRLIRAG
                     SPVPAGLVLLPRAFDGDRVTAPVGDELRAELARFPAGRTFAVRSSARVEDSAEASFAG
                     AFDSVVDVAADDLEAAIAQVRASARAVRVGAYSEAVAADRADLAVVLQTMVDADLAGV
                     LFTVHPLTGALDQMLGSLVHGAGEALVSGADTGQQFTLARPSGAYTGPDELRPFAQAL
                     HDEAHRIETTFDGVPQDIEWVIAGGRLSIVQARPITTLNPWNPRTTERNDSLAGTCLW
                     SATNLSEANPVPQTPLTISLTRYQQANGGPSMALRGRELAGSIGGRPYANLTVQITAR
                     RGKGGKQDPREVYRKLAGWWGALPENVPVPLLPMTPDDWQRAGPPLLGSVVRMQAVRF
                     GLPRFLREHARRCDELMAQVRNCSAPAELLRLWNDEVLGFGIDSFWAVIASGSDSAAG
                     VEQQIRDRYGADVAAGLMSNLSGFAGDLASMGPAVGLGEVLAGRMSRDEYLRRFGHRG
                     VNEAELAWPRPCEDPGWLDRMLEGATDSATLRARRVHQQQAFDQALDAVRARESGRVR
                     RLERRLTKVARRAALREAVRSEGVRTIGVTRAWALRCGDLLGIG"
     gene            complement(1991..2230)
                     /locus_tag="PLF56_12945"
     CDS             complement(1991..2230)
                     /locus_tag="PLF56_12945"
                     /inference="COORDINATES: protein motif:HMM:TIGR01439.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="AbrB/MazE/SpoVT family DNA-binding
                     domain-containing protein"
                     /protein_id="HOQ54504.1"
                     /translation="MMVREQDPNKKFYGAVTVSDRGQIAIPVQARRDFGIEVGDKLLV
                     FGDLQHGIALGRADDIIARHPGSAAIFGNDPDVAD"
BASE COUNT          380 a          890 c          843 g          341 t
ORIGIN      
        1 accgatgccg agcaggtcgc cgcagcgcag tgcccacgcc cgggtgacgc cgatggtgcg
       61 caccccctca gagcggacgg cctcgcgaag cgccgcccgg cgggcgacct tcgtcaatcg
      121 ccgttcgagc cggcgcaccc ggccggattc tcgggcacgg acggcgtcca gggcctggtc
      181 gaaagcctgt tgctggtgga cgcgccgggc gcgcagagtc gcgctgtcgg tggccccctc
      241 aagcattcgg tcgagccagc ccgggtcctc gcacggccgc ggccaggcga gttcggcctc
      301 gttgacgccg cgatgcccga accggcgcag gtactcatcg cgcgacatcc ggccggccag
      361 cacctcgccc aacccgacgg cggggcccat gctggccagg tcgccggcaa atccggacag
      421 gttggacatc aggccggcgg cgacgtccgc gccgtaccgg tcgcggatct gctgctcgac
      481 gccggcggcc gaatcggacc cggacgcgat caccgcccag aacgaatcga tgccgaaacc
      541 gaggacttcg tcgttccaca agcgcagcaa ctctgccggg gccgaacagt tccgtacttg
      601 cgccatcaac tcatcgcagc gacgggcgtg ctcgcgaagg aaccgtggca gcccgaaccg
      661 gacggcctgc atgcgcacca cgctgcccag caggggtggg ccggcgcgct gccagtcgtc
      721 cggggtcatc ggcagcagcg gcaccggaac gttctccggc agggcacccc accagccggc
      781 cagtttgcgg tacacctcgc gtggatcctg cttgccgccc ttgccccgcc gggccgtgat
      841 ctgcaccgtg aggttcgcgt agggccggcc gccgatcgac ccggccagtt cgcgtccacg
      901 cagggccatg gacggtccgc cgttggcctg ctggtagcgc gtcagcgaga tcgtcagcgg
      961 ggtctgcggc accgggttgg cctcgctgag gttggtcgcc gaccacagac aggtgccggc
     1021 cagcgagtcg ttccgctcgg tcgtgcgggg gttccaaggg ttgagcgtgg tgatcggacg
     1081 cgcctgcacg atcgacagcc gtcccccggc gatcacccac tcgatgtctt gcggcacgcc
     1141 gtcgaaggtg gtctcgatgc ggtgtgcctc gtcgtgcaac gcctgagcga agggccgcag
     1201 ttcgtccggc ccggtgtagg cgccggacgg ccgtgcgaga gtgaactgct gcccggtatc
     1261 ggcacccgag acgagcgcct cgcccgcacc gtggacgagc gagccgagca tctggtcgag
     1321 cgccccggtc agggggtgga cggtgaacaa caccccggcg agatcggcat cgaccatggt
     1381 ctgcaacacc acggccaggt cggcccggtc ggccgcgacc gcctcgctgt aggcgccgac
     1441 ccgcaccgcg cgggccgagg cgcgcacctg ggcgatggcc gcctcgaggt cgtccgcggc
     1501 gacgtcgacg accgagtcaa aggccccggc gaacgatgcc tcggccgaat cctcgactct
     1561 cgcggaggac cgaacggcga acgtgcgtcc ggccgggaac ctggccaact cggcacgcag
     1621 ttcgtcgccc accggggcgg tcacgcgatc gccgtcgaaa gcccgcggca gcagcaccag
     1681 cccggccggc accggactgc ccgcgcggat caaccggctg agcgaacggg ccttgccacc
     1741 cgcgctgagt tcgtcgggcg gtaggtgctc cagccgatac aggggtcgtc cggacggcgc
     1801 gtagccggcc cgggcgagcc gacgggcgct cgcccgtccc gcagtgaagc cggcgaacca
     1861 gcccagcgtg cctgtcagcg gcgcgaccag tagcatccag ggcccgaacc cggccgccca
     1921 ggcacccgca agtccaccgg cggcggccgc cgcattggcg gtgatcctcg tcagccggac
     1981 ggtcgagcgc tcagtcagca acatcgggat cgttgccgaa gatggcagcg ctgccgggat
     2041 gccgggcgat gatgtcatcg gccctgccga gcgcgatgcc gtgctgcagg tcgccgaaga
     2101 cgagcaactt gtcgcccacc tcgatgccga agtcgcgccg ggcctgcacc gggatcgcga
     2161 tctgcccccg atcgctgacc gtcaccgcac cgtagaactt cttgttcgga tcctgctcgc
     2221 gcaccatcat gcctccaagg aatatcttgt attcaagact agtgcaccgc gctgtcgcac
     2281 cgcttgtcgt tggagtgacc ccatcgctga tcgagccagc tcagatttca cgcatcggcc
     2341 tcgaccagcg gcgcactcgt catcccgaga tctcgacgca cccagcgatc ccggcgtgcg
     2401 ccgggatgac gaggggtcac gatattcacg cgacggggtc gccactgtgt tcag
//
LOCUS       DAOSUG010000477         2446 bp    DNA     linear   ENV 10-JUN-2024
DEFINITION  MAG TPA_asm: Micropruina sp. isolate AMR_MDS_2195
            NODE_17400_length_2446_cov_3.816395, whole genome shotgun sequence.
ACCESSION   DAOSUG010000477 DAOSUG010000000
VERSION     DAOSUG010000477.1
DBLINK      BioProject: PRJNA925477
            BioSample: SAMN32864262
            Sequence Read Archive: SRR4236673
KEYWORDS    WGS; ENV; Metagenome Assembled Genome; MAG; Third Party Data; TPA;
            TPA:assembly.
SOURCE      Micropruina sp. (wastewater metagenome)
  ORGANISM  Micropruina sp.
            Bacteria; Bacillati; Actinomycetota; Actinomycetes;
            Propionibacteriales; Nocardioidaceae; Micropruina.
REFERENCE   1  (bases 1 to 2446)
  AUTHORS   Abdulkadir,N., Saraiva,J.P., Zhang,J., Stolte,S., Gillor,O.,
            Harms,H. and Rocha,U.
  TITLE     Genome-centric analyses of 165 metagenomes show that mobile genetic
            elements are crucial for the transmission of antimicrobial
            resistance genes to pathogens in activated sludge and wastewater
  JOURNAL   Microbiol Spectr 12 (3), e0291823 (2024)
   PUBMED   38289113
REFERENCE   2  (bases 1 to 2446)
  AUTHORS   Abdulkadir,N.
  TITLE     Direct Submission
  JOURNAL   Submitted (24-MAR-2023) Environmental Microbiology, Helmholtz
            Center for Environmental Research -UFZ, Permoserstr 15, Leipzig
            04318, Germany
COMMENT     The annotation was added by the NCBI Prokaryotic Genome Annotation
            Pipeline (PGAP). Information about PGAP can be found here:
            https://www.ncbi.nlm.nih.gov/genome/annotation_prok/
            
            ##Genome-Assembly-Data-START##
            Assembly Method        :: SPAdes v. 3.11.1
            Genome Representation  :: Full
            Expected Final Version :: Yes
            Genome Coverage        :: Not Applicable
            Sequencing Technology  :: Illumina MiSeq
            ##Genome-Assembly-Data-END##
            
            ##Genome-Annotation-Data-START##
            Annotation Provider               :: NCBI
            Annotation Date                   :: 05/04/2023 04:57:10
            Annotation Pipeline               :: NCBI Prokaryotic Genome
                                                 Annotation Pipeline (PGAP)
            Annotation Method                 :: Best-placed reference protein
                                                 set; GeneMarkS-2+
            Annotation Software revision      :: 6.5
            Features Annotated                :: Gene; CDS; rRNA; tRNA; ncRNA
            Genes (total)                     :: 2,776
            CDSs (total)                      :: 2,750
            Genes (coding)                    :: 2,714
            CDSs (with protein)               :: 2,714
            Genes (RNA)                       :: 26
            tRNAs                             :: 24
            ncRNAs                            :: 2
            Pseudo Genes (total)              :: 36
            CDSs (without protein)            :: 36
            Pseudo Genes (ambiguous residues) :: 0 of 36
            Pseudo Genes (frameshifted)       :: 2 of 36
            Pseudo Genes (incomplete)         :: 32 of 36
            Pseudo Genes (internal stop)      :: 2 of 36
            ##Genome-Annotation-Data-END##
FEATURES             Location/Qualifiers
     source          1..2446
                     /organism="Micropruina sp."
                     /mol_type="genomic DNA"
                     /submitter_seqid="NODE_17400_length_2446_cov_3.816395"
                     /isolate="AMR_MDS_2195"
                     /isolation_source="Activated sludge of municipal
                     wastewater treatment plants"
                     /db_xref="taxon:2737536"
                     /environmental_sample
                     /geo_loc_name="Canada"
                     /lat_lon="49.25 N 123.1 W"
                     /collection_date="2015-06-16"
                     /metagenome_source="wastewater metagenome"
                     /note="metagenomic"
     gene            <1..404
                     /locus_tag="PLF56_12950"
     CDS             <1..404
                     /locus_tag="PLF56_12950"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_013862926.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_component: GO:0016020 - membrane [Evidence IEA];
                     GO_process: GO:0055085 - transmembrane transport [Evidence
                     IEA]"
                     /codon_start=3
                     /transl_table=11
                     /product="ABC transporter permease subunit"
                     /protein_id="HOQ54505.1"
                     /translation="ALAFVTLPFVVRSVQPVLIEVDRDVEQAAASLGASNWTIFRRVV
                     LPTLAPSIISGGGLAFARAIGEYGSVVLIGGNIPRETQVASQYIQQQIEIDRPVNAAA
                     VSVALLAISFATLLILRFFASRSQEREEKAT"
     gene            401..1285
                     /gene="cysW"
                     /locus_tag="PLF56_12955"
     CDS             401..1285
                     /gene="cysW"
                     /locus_tag="PLF56_12955"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_008356462.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_component: GO:0005886 - plasma membrane [Evidence IEA];
                     GO_component: GO:0055052 - ATP-binding cassette (ABC)
                     transporter complex, substrate-binding subunit-containing
                     [Evidence IEA];
                     GO_function: GO:0015419 - ABC-type sulfate transporter
                     activity [Evidence IEA];
                     GO_process: GO:0008272 - sulfate transport [Evidence IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="sulfate ABC transporter permease subunit CysW"
                     /protein_id="HOQ54506.1"
                     /translation="MKLSTPARLTLRIIALVYLTVLLAVPIVTILWRTFEPGFAAFWA
                     SIRTPAAISALNLSLLIVAITVPINVVFGVVTALALVRGRFPGRRLLSGVVDLPFAVS
                     PIVVGVSLIMLWGTHGWFGGLENLGIQVIFGLPGMVIATIFVTLPFVVREVEPVLHEI
                     GTDQEQAAATLGASRWQTFARITLPAIRWGLTYGIVLTVARALGEFGAVIMVSSGFPG
                     VSQTLTLLVHSRYIDDHNVHGAYSAATLLMFLAIITLLAMSLLDRKRPEGQSAALPAT
                     IAPAPTPDPAEETSRSAA"
     gene            1282..2289
                     /locus_tag="PLF56_12960"
     CDS             1282..2289
                     /locus_tag="PLF56_12960"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_013161994.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="TOBE-like domain-containing protein"
                     /protein_id="HOQ54507.1"
                     /translation="MISVTNANKNYGTFKALDDVSLEIPSGTLTALLGPSGSGKSTLL
                     RSIAGLEELDSGSVVIAGNDVTHVPPQQRGIGFVFQHYAAFKHMTVRDNVAFGLTIRK
                     RPKAEITKRVDELLEVVGLAGFQHRYPAQLSGGQRQRMALARALAVDPQVLLLDEPFG
                     ALDAKVRDDLRRWLRRLHDEVHVTTVLVTHDQEEALDVADRIAVLNGGRIEQIGTPDE
                     LYEQPANPFVMSFLGSVSRLNGEVARPHDLRIERDQQRAADEARSSGVHVTEATVERL
                     VRLGFEVRIELSHVHTGEQFFAQLTRAQAEDLRLAVGDRVYVLPTRTLPTEEQFPALV
                     V"
BASE COUNT          346 a          853 c          814 g          433 t
ORIGIN      
        1 tggcgctggc cttcgtcacc ctgccgttcg tggtgcgttc ggttcagccg gtgctgatcg
       61 aggtcgaccg cgacgtcgag caggccgccg cttcgctcgg tgccagcaac tggacgatct
      121 ttcgccgcgt cgtgctgccc acgctggccc cgtccatcat ctcgggcggg ggcctggcct
      181 tcgctcgcgc aatcggcgag tacggctcgg tggtgctgat cggcggcaac attccgcgtg
      241 agacccaggt ggcctcgcag tacattcagc agcagatcga gatcgaccgc ccggtgaacg
      301 cggcggcggt gtcggtggct ctgctcgcga tctcgttcgc gacgctgctg attctcaggt
      361 tcttcgcctc gcgcagccaa gagcgtgagg agaaggcaac atgaagctct ccacacccgc
      421 ccgcctcacc ctgcgcatca tcgcgctggt gtacctgacc gtgctgttgg cggtgccgat
      481 cgtgaccatt ctgtggcgca cgttcgaacc cggcttcgcg gcgttctggg cctcgatccg
      541 cacgcccgcg gcgatctctg ccttgaacct gtcgctgctg atcgtggcga tcacggtgcc
      601 gatcaacgtc gtgttcggcg tggtcaccgc gctcgccctg gtccgcggac ggttccccgg
      661 ccgccggctg ctctcgggcg tggtcgacct gccgttcgcg gtctcgccga tcgtggtcgg
      721 cgtgtcgctg atcatgctct ggggcacgca tggctggttc ggcggcctgg agaacctggg
      781 catccaggtg atcttcggac tgcccggcat ggtgatcgcc accatcttcg tgaccctgcc
      841 gttcgtcgtc cgcgaggtcg agccggtgct gcacgagata ggcaccgatc aggagcaggc
      901 ggccgccacc ctcggcgcgt cccggtggca gaccttcgca cgcatcacgc tgcccgccat
      961 ccgctggggg ctcacctacg gcatcgtgct caccgtggct cgtgcgctgg gcgagttcgg
     1021 tgccgtgatc atggtgagct ccggctttcc gggcgtttcg cagacgctga ccctgctcgt
     1081 ccacagccgc tacatcgacg accacaacgt gcacggcgcc tactcggccg ccacgctgtt
     1141 gatgttcctg gccatcatca ccctgctcgc gatgagcctg ctcgaccgca agcgacccga
     1201 ggggcagtcg gccgcactac cggccaccat cgccccggcc cccacacccg accccgctga
     1261 agaaacttcc aggagtgcgg catgatctcc gtcaccaacg ccaacaagaa ctacggcact
     1321 ttcaaggccc tggacgatgt gtcgctcgag attccctcgg gcacgctgac cgccctgctc
     1381 ggaccgtccg gttcgggcaa gtcgacgctg ctgcgttcga tcgcgggcct ggaggagctc
     1441 gactcgggct cggtggtgat cgcaggcaac gacgtcaccc acgttccgcc gcagcagcga
     1501 gggatcgggt tcgtgtttca gcactacgcc gcgttcaagc acatgacggt gcgcgacaac
     1561 gtcgccttcg gcctgaccat tcgcaagcgg cccaaggccg agatcaccaa gcgtgtggac
     1621 gaactgctcg aggtggtggg gctggcgggc tttcagcacc gctacccggc gcaactctcg
     1681 ggcgggcagc ggcagcgcat ggcgttggcc cgggcgttgg cggtcgatcc gcaggtgttg
     1741 ctgctcgacg agccgttcgg tgccctggac gccaaggtgc gcgacgacct gcggcgctgg
     1801 ctgcgccggc tgcatgacga agtgcacgtc accacggtgc tggtcaccca cgatcaggag
     1861 gaggcgctcg acgtggccga ccggatcgcg gtgctgaacg gcggccggat cgagcagatc
     1921 ggcacccccg acgagctgta cgagcaaccg gccaacccgt tcgtgatgtc gttcctgggg
     1981 tcggtgtcga ggctgaacgg cgaggtggca cgtccgcacg acctgcgcat cgagcgcgac
     2041 cagcagcggg ccgccgacga ggcgcgaagc tcgggggtgc acgtcaccga ggcgacggtc
     2101 gaacggctcg ttcggttggg tttcgaggtg cgcatcgagt tgagccacgt gcatacgggc
     2161 gagcagttct tcgcccagct gactcgggcc caggcagagg atcttcggct cgccgtcggc
     2221 gaccgggtct acgtgctgcc gacccgcacc ctgccgaccg aggagcagtt tccggccctg
     2281 gtcgtctgag cctcgatcca ccgatctgcg cctaccgcgc ggcccatccg gccaccctgg
     2341 ctgcgtcggc cacgctcacc agacgccggt atgccttcgc gcggaccgtc gtgccaggcc
     2401 acccgtctgg tgccgctcgc gacggcgccc atcggtggat ccaggc
//
LOCUS       DAOSUG010000478         2446 bp    DNA     linear   ENV 10-JUN-2024
DEFINITION  MAG TPA_asm: Micropruina sp. isolate AMR_MDS_2195
            NODE_17404_length_2446_cov_2.808867, whole genome shotgun sequence.
ACCESSION   DAOSUG010000478 DAOSUG010000000
VERSION     DAOSUG010000478.1
DBLINK      BioProject: PRJNA925477
            BioSample: SAMN32864262
            Sequence Read Archive: SRR4236673
KEYWORDS    WGS; ENV; Metagenome Assembled Genome; MAG; Third Party Data; TPA;
            TPA:assembly.
SOURCE      Micropruina sp. (wastewater metagenome)
  ORGANISM  Micropruina sp.
            Bacteria; Bacillati; Actinomycetota; Actinomycetes;
            Propionibacteriales; Nocardioidaceae; Micropruina.
REFERENCE   1  (bases 1 to 2446)
  AUTHORS   Abdulkadir,N., Saraiva,J.P., Zhang,J., Stolte,S., Gillor,O.,
            Harms,H. and Rocha,U.
  TITLE     Genome-centric analyses of 165 metagenomes show that mobile genetic
            elements are crucial for the transmission of antimicrobial
            resistance genes to pathogens in activated sludge and wastewater
  JOURNAL   Microbiol Spectr 12 (3), e0291823 (2024)
   PUBMED   38289113
REFERENCE   2  (bases 1 to 2446)
  AUTHORS   Abdulkadir,N.
  TITLE     Direct Submission
  JOURNAL   Submitted (24-MAR-2023) Environmental Microbiology, Helmholtz
            Center for Environmental Research -UFZ, Permoserstr 15, Leipzig
            04318, Germany
COMMENT     The annotation was added by the NCBI Prokaryotic Genome Annotation
            Pipeline (PGAP). Information about PGAP can be found here:
            https://www.ncbi.nlm.nih.gov/genome/annotation_prok/
            
            ##Genome-Assembly-Data-START##
            Assembly Method        :: SPAdes v. 3.11.1
            Genome Representation  :: Full
            Expected Final Version :: Yes
            Genome Coverage        :: Not Applicable
            Sequencing Technology  :: Illumina MiSeq
            ##Genome-Assembly-Data-END##
            
            ##Genome-Annotation-Data-START##
            Annotation Provider               :: NCBI
            Annotation Date                   :: 05/04/2023 04:57:10
            Annotation Pipeline               :: NCBI Prokaryotic Genome
                                                 Annotation Pipeline (PGAP)
            Annotation Method                 :: Best-placed reference protein
                                                 set; GeneMarkS-2+
            Annotation Software revision      :: 6.5
            Features Annotated                :: Gene; CDS; rRNA; tRNA; ncRNA
            Genes (total)                     :: 2,776
            CDSs (total)                      :: 2,750
            Genes (coding)                    :: 2,714
            CDSs (with protein)               :: 2,714
            Genes (RNA)                       :: 26
            tRNAs                             :: 24
            ncRNAs                            :: 2
            Pseudo Genes (total)              :: 36
            CDSs (without protein)            :: 36
            Pseudo Genes (ambiguous residues) :: 0 of 36
            Pseudo Genes (frameshifted)       :: 2 of 36
            Pseudo Genes (incomplete)         :: 32 of 36
            Pseudo Genes (internal stop)      :: 2 of 36
            ##Genome-Annotation-Data-END##
FEATURES             Location/Qualifiers
     source          1..2446
                     /organism="Micropruina sp."
                     /mol_type="genomic DNA"
                     /submitter_seqid="NODE_17404_length_2446_cov_2.808867"
                     /isolate="AMR_MDS_2195"
                     /isolation_source="Activated sludge of municipal
                     wastewater treatment plants"
                     /db_xref="taxon:2737536"
                     /environmental_sample
                     /geo_loc_name="Canada"
                     /lat_lon="49.25 N 123.1 W"
                     /collection_date="2015-06-16"
                     /metagenome_source="wastewater metagenome"
                     /note="metagenomic"
     gene            complement(<1..1160)
                     /locus_tag="PLF56_12965"
     CDS             complement(<1..1160)
                     /locus_tag="PLF56_12965"
                     /inference="COORDINATES: protein motif:HMM:NF039718.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="DUF6297 family protein"
                     /protein_id="HOQ54508.1"
                     /translation="MSKKSRKLINAAQSKPEPSAPEAVVEPAEPEQTFWFLPDVAPEP
                     VDERDLHEAMKQWRHGRATRTIGEAIQNAYVAIFTIVMIGAMITNLVLKAQSSMAGCA
                     ELSCQSARTLVPWATWFALLGLALGVSRLFGPVLVSAAEGFWLMDAPIERSRLLSGRL
                     RLAVLAFGLIAAVLGALTAALSGSSPLEIALWALADGLGAAGVTAFAAAEQTRERVRL
                     TRLVQTVLATLALATLLLTVAVAAGWLTLSLPQETSLLVPAVAAALGLVLLIVELLVA
                     RRRLDQIRRARLVSGGSLVAGMQGAMYALDFGLVRDIVVERAAVERGHVNPTPGRGLG
                     LQALLWRDLQRLTRFPRPLVGLAASVVAPYALDALGMTTLNPFISGLILVVVL"
     gene            complement(1157..1819)
                     /locus_tag="PLF56_12970"
     CDS             complement(1157..1819)
                     /locus_tag="PLF56_12970"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_014847381.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_function: GO:0005524 - ATP binding [Evidence IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="ABC transporter ATP-binding protein"
                     /protein_id="HOQ54509.1"
                     /translation="MAKRSTVLKVAGMSKSFGPVVIVDNFNLEVREGEAIALTGRNGA
                     GKSTILRCIVGADQPDEGTVEVDGIRMSETNPEVRRAVATVIDDLDFFPDLSVVEHLD
                     LLARAHGIADAEQAVDEVLHEVQLVPQSGQLPGTLSSGQRRRLALATAFIRPRRLLVL
                     DEPEQRLDVEGVEWLAARLAAERNRGLAIVMASHEPKLVQSVATRIVRIGDSVAVDEV
                     AG"
     gene            1907..>2446
                     /locus_tag="PLF56_12975"
     CDS             1907..>2446
                     /locus_tag="PLF56_12975"
                     /EC_number="2.1.1.-"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_014847383.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_function: GO:0008168 - methyltransferase activity
                     [Evidence IEA];
                     GO_process: GO:0008299 - isoprenoid biosynthetic process
                     [Evidence IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="ubiquinone/menaquinone biosynthesis
                     methyltransferase"
                     /protein_id="HOQ54510.1"
                     /translation="MVDTGRARATLAKRRADVAAMFDGVAARYDLVNDLLTGGLDRGW
                     RRAVVAAVAPQSGQRILDLAAGTGTSSKPFADAGALVVAADLSLGMLQVGAERQPQLT
                     FVNADALALPFADAAFDAVTISFGLRNVEDTDAALAELRRVTRPGGRIVICEFSTPTF
                     APLRWLYANVALRALPLIAR"
BASE COUNT          370 a          886 c          864 g          326 t
ORIGIN      
        1 agcaccacga ccaggatcag ccccgaaatg aacgggttga gcgtggtcat gcccagagcg
       61 tccaaggcgt acggggcgac caccgacgcg gccagcccga ccagcggcct cgggaaccgg
      121 gtgagccgct gcaggtcgcg ccacagcagc gcctgcaggc ccagcccccg gccgggggtg
      181 gggttgacgt ggccacgctc gacggcggcc cgctcgacca cgatgtcgcg caccagaccg
      241 aagtcgagcg cgtacatggc gccctgcatg cccgccacca gcgaaccgcc cgacacgagc
      301 cgcgcccggc ggatctgatc gagccggcgg cgggccacca gcagttcgac gatcaggagc
      361 acaagcccga gcgcggccgc gacggcgggc accagcagcg acgtctcttg cggcagcgag
      421 agggtcagcc aaccggccgc cacggccacg gtgagcagca gggtggccag cgccagggtg
      481 gccagcacgg tctgcaccag ccgggtcagc cgcacgcgtt cgcgggtctg ctctgcggcg
      541 gcgaacgcgg tcaccccggc cgcgcccagc ccgtccgcca gggcccacag ggcgatctcg
      601 agcgggctgg agccgctcag cgccgcggtc agggcaccca gcaccgcggc gatcaggccg
      661 aaggcgagca cggccagccg cagccgtccg ctcagcagcc gcgaacgctc gataggggcg
      721 tccatcagcc agaagccttc ggcggccgac acgagcaccg gcccgaacag ccggctgacg
      781 cccagggcca ggccgagcag cgcgaaccag gtggcccacg gcaccagcgt gcgggccgac
      841 tggcacgaca actcggcaca gccggccatc gacgactgcg ccttgagcac caggttggtg
      901 atcatggcgc cgatcatcac gatcgtgaag atggccacgt aggcgttctg aatggcttcg
      961 ccgatggttc gggtggcgcg accgtgccgc cactgcttca tcgcctcgtg caggtcgcgc
     1021 tcgtccaccg gctcgggggc cacgtcgggc agaaaccaga acgtctgctc gggttcggcc
     1081 ggctcgacga ccgcttcggg cgcggacggc tcgggcttgc tctgcgccgc gttgatcagc
     1141 ttgcgggact tcttgctcac ccggcgacct cgtcgacggc cacgctgtca ccgatgcgca
     1201 cgatgcgggt ggccacgctc tgcacgagtt tgggctcgtg gctcgccatc acgatcgcca
     1261 gcccgcggtt gcgttcggcg gccagccgcg cggccagcca ctcgacaccc tcgacgtcga
     1321 gccgctgctc gggttcgtcg agcaccagca gccggcgcgg gcggatgaac gcggtggcca
     1381 gcgccagccg gcggcgctga ccggacgaca gcgtgcccgg caactgcccc gactgcggca
     1441 ccagctgcac ctcgtgcagc acctcgtcga cggcctgctc ggcgtcggcg atgccgtgcg
     1501 cccgggccag caggtcgagg tgctcgacca cggagaggtc gggaaagaag tcgaggtcgt
     1561 cgatcacggt ggccacggcg cgacgcacct cgggattggt ctcgctcatg cgaatgccgt
     1621 cgacctcgac ggtgccctcg tcgggctggt cggcaccgac gatgcagcgc agaatggtcg
     1681 acttgccggc cccgttgcgt ccggtcaggg cgatcgcctc gccctcacgc acctcgaggt
     1741 tgaagttgtc gacgatgacg accgggccga acgacttgct catcccggcc accttgagca
     1801 ccgtgctgcg tttggccatg ggcccgattc tccaccacaa cgaaagattg ctacgcatcg
     1861 tccgccgggt cggtgagtgt cggtgcggcg tggcaagcta gccgccgtgg ttgacaccgg
     1921 acgagcaagg gcgacgctgg ccaagcgcag agccgacgtg gcggcgatgt tcgacggggt
     1981 ggcggcccgc tacgacctgg tcaacgacct gctcaccggc gggctcgacc ggggttggcg
     2041 ccgggcggtc gtcgctgcgg tggcgccgca atccggtcag cgcattctcg acctggccgc
     2101 cggcaccggc acgtcgtcga agccgttcgc cgacgcgggt gccctggtgg tggcggccga
     2161 cctgagcctc ggcatgctgc aggtgggcgc cgaacggcag ccgcagctca ccttcgtgaa
     2221 cgccgacgcg ctggccctgc cgttcgccga cgcggcgttc gacgcggtga ccatctcgtt
     2281 cgggctgcgc aacgtcgaag acaccgacgc cgctctggcc gaactgcgcc gggtgacccg
     2341 gcccggcggt cgcatcgtga tctgcgagtt cagcacgccg accttcgcgc cgctgcgctg
     2401 gctgtacgcc aacgtcgctc tccgggcgct gccgctgatc gcccgg
//
LOCUS       DAOSUG010000479         2444 bp    DNA     linear   ENV 10-JUN-2024
DEFINITION  MAG TPA_asm: Micropruina sp. isolate AMR_MDS_2195
            NODE_17421_length_2444_cov_4.882378, whole genome shotgun sequence.
ACCESSION   DAOSUG010000479 DAOSUG010000000
VERSION     DAOSUG010000479.1
DBLINK      BioProject: PRJNA925477
            BioSample: SAMN32864262
            Sequence Read Archive: SRR4236673
KEYWORDS    WGS; ENV; Metagenome Assembled Genome; MAG; Third Party Data; TPA;
            TPA:assembly.
SOURCE      Micropruina sp. (wastewater metagenome)
  ORGANISM  Micropruina sp.
            Bacteria; Bacillati; Actinomycetota; Actinomycetes;
            Propionibacteriales; Nocardioidaceae; Micropruina.
REFERENCE   1  (bases 1 to 2444)
  AUTHORS   Abdulkadir,N., Saraiva,J.P., Zhang,J., Stolte,S., Gillor,O.,
            Harms,H. and Rocha,U.
  TITLE     Genome-centric analyses of 165 metagenomes show that mobile genetic
            elements are crucial for the transmission of antimicrobial
            resistance genes to pathogens in activated sludge and wastewater
  JOURNAL   Microbiol Spectr 12 (3), e0291823 (2024)
   PUBMED   38289113
REFERENCE   2  (bases 1 to 2444)
  AUTHORS   Abdulkadir,N.
  TITLE     Direct Submission
  JOURNAL   Submitted (24-MAR-2023) Environmental Microbiology, Helmholtz
            Center for Environmental Research -UFZ, Permoserstr 15, Leipzig
            04318, Germany
COMMENT     The annotation was added by the NCBI Prokaryotic Genome Annotation
            Pipeline (PGAP). Information about PGAP can be found here:
            https://www.ncbi.nlm.nih.gov/genome/annotation_prok/
            
            ##Genome-Assembly-Data-START##
            Assembly Method        :: SPAdes v. 3.11.1
            Genome Representation  :: Full
            Expected Final Version :: Yes
            Genome Coverage        :: Not Applicable
            Sequencing Technology  :: Illumina MiSeq
            ##Genome-Assembly-Data-END##
            
            ##Genome-Annotation-Data-START##
            Annotation Provider               :: NCBI
            Annotation Date                   :: 05/04/2023 04:57:10
            Annotation Pipeline               :: NCBI Prokaryotic Genome
                                                 Annotation Pipeline (PGAP)
            Annotation Method                 :: Best-placed reference protein
                                                 set; GeneMarkS-2+
            Annotation Software revision      :: 6.5
            Features Annotated                :: Gene; CDS; rRNA; tRNA; ncRNA
            Genes (total)                     :: 2,776
            CDSs (total)                      :: 2,750
            Genes (coding)                    :: 2,714
            CDSs (with protein)               :: 2,714
            Genes (RNA)                       :: 26
            tRNAs                             :: 24
            ncRNAs                            :: 2
            Pseudo Genes (total)              :: 36
            CDSs (without protein)            :: 36
            Pseudo Genes (ambiguous residues) :: 0 of 36
            Pseudo Genes (frameshifted)       :: 2 of 36
            Pseudo Genes (incomplete)         :: 32 of 36
            Pseudo Genes (internal stop)      :: 2 of 36
            ##Genome-Annotation-Data-END##
FEATURES             Location/Qualifiers
     source          1..2444
                     /organism="Micropruina sp."
                     /mol_type="genomic DNA"
                     /submitter_seqid="NODE_17421_length_2444_cov_4.882378"
                     /isolate="AMR_MDS_2195"
                     /isolation_source="Activated sludge of municipal
                     wastewater treatment plants"
                     /db_xref="taxon:2737536"
                     /environmental_sample
                     /geo_loc_name="Canada"
                     /lat_lon="49.25 N 123.1 W"
                     /collection_date="2015-06-16"
                     /metagenome_source="wastewater metagenome"
                     /note="metagenomic"
     gene            861..1058
                     /locus_tag="PLF56_12980"
     CDS             861..1058
                     /locus_tag="PLF56_12980"
                     /inference="COORDINATES: ab initio
                     prediction:GeneMarkS-2+"
                     /note="Derived by automated computational analysis using
                     gene prediction method: GeneMarkS-2+."
                     /codon_start=1
                     /transl_table=11
                     /product="hypothetical protein"
                     /protein_id="HOQ54511.1"
                     /translation="MTYTVTVDGTFESGGTIVCSMGVTYTNSAIQPRKGKHRVEVMFG
                     PEVVNTNWAYIRIIPTRLVDP"
     gene            1198..1686
                     /gene="rpiB"
                     /locus_tag="PLF56_12985"
     CDS             1198..1686
                     /gene="rpiB"
                     /locus_tag="PLF56_12985"
                     /EC_number="5.3.1.6"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:NP_418514.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_function: GO:0004751 - ribose-5-phosphate isomerase
                     activity [Evidence IEA];
                     GO_process: GO:0009052 - pentose-phosphate shunt,
                     non-oxidative branch [Evidence IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="ribose 5-phosphate isomerase B"
                     /protein_id="HOQ54512.1"
                     /translation="MRIVAHSDHAGVEIRHEMIDQAREMGHEVDDLGPAAGEKVDYPY
                     AGAIVGHKVASGEYDLGLLCCGTGVGISMAANKVPGARAVVCSEAYTARLSREHNASN
                     IVSVGARVVGPGEARMIVEAFLLAEPAGGRHAERVKMINALDDDAISLDALYQMGPAA
                     HQ"
     gene            2054..2425
                     /gene="rpsL"
                     /locus_tag="PLF56_12990"
     CDS             2054..2425
                     /gene="rpsL"
                     /locus_tag="PLF56_12990"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_020488528.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_component: GO:0000314 - organellar small ribosomal
                     subunit [Evidence IEA];
                     GO_component: GO:0022627 - cytosolic small ribosomal
                     subunit [Evidence IEA];
                     GO_function: GO:0003735 - structural constituent of
                     ribosome [Evidence IEA];
                     GO_process: GO:0006412 - translation [Evidence IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="30S ribosomal protein S12"
                     /protein_id="HOQ54513.1"
                     /translation="MPTIQQLVRKGRTDKVSKNKTPALKGSPQRRGVCTRVYTTTPKK
                     PNSALRKVARVRLSSGVEVTAYIPGVGHNLQEHSMVLVRGGRVKDLPGVRYKIIRGSL
                     DTQGVKNRKQARSRYGAKKEK"
BASE COUNT          447 a          753 c          823 g          421 t
ORIGIN      
        1 catcacccca tcattggaga cccatgagac attcacgctc actcataacg ctgggctcaa
       61 ctctgacgtc ttgtgctgga ataaccgcgg gaactgcgtg ggctgattca cgcctaccgg
      121 tcgcaccctg tgcccaatca tcaccaactg tgctggacag accggccggg acgggcgtga
      181 ctccgctcac ttgctgcgtc cagtcgaaac cagcactcct tcaaagtggt gaattcacgc
      241 attccgtttg ccggagtgtc ggccgtcacg aactggcccg ggccggtgtc cagcacactc
      301 agtcgcgagg tttcgggaac tgcatcattc aaggtgactg ccgcagcgga ggcggcggcc
      361 ggggttgttc tagccaaagc gaaggcgtcg ctgtctttgg agattcagct gagcaacacc
      421 cacaaccacg cgggaatttc gaccactttg gctgcgggca aaggccagta gtcacatgct
      481 cgctacgtct ggtggagcaa gaaggtcacg taccggaagt accgggtcca cgcgaattgc
      541 acgagtacta ccattagtac gggtaccatc aactatccca ctcccaatga agggtggtac
      601 acgtgggtcg actcatcacc ctctgtgtga tctcaacatt gttggtcggg tgcacatcgg
      661 ttccgccaac acccacgagc agcacggtgg ttcccccggt cagcgcatcc ccgtgggccg
      721 gcccgcccag tcccattcgc gaagaggtcg cgcgggacct ctggagttcc ggtgcgcgca
      781 ccacgactcc tggcgaagcc gacctcggca atgagcagtg ggtcgcttcg ggcaggtgcg
      841 atggcggtgg tgatcttgcg gtgacctaca ccgtcacggt cgacggcacc ttcgagagcg
      901 gtggaacgat cgtgtgctcg atgggcgtca cctacacgaa ctcggcgatc cagccccgca
      961 agggcaagca tcgagtcgag gtgatgttcg gtcccgaggt cgtcaacacc aactgggcgt
     1021 acattcggat aattcccacc aggctggtcg atccctgagt gagtgggccg catcaagcgt
     1081 ccacctcgct caccttcggg tcgtcggccc ggccgttcgc tgggatgaca tggagtgggt
     1141 cgggatgccg ggagcggcca tgctggccct cggggctggg cggcctaggg tgggggcatg
     1201 cgcatcgttg cccacagtga ccatgccggc gtcgagatcc ggcacgagat gatcgaccag
     1261 gcccgtgaga tgggccacga ggtcgatgac ctcgggcctg ccgcgggcga gaaggtcgac
     1321 tatccctacg cgggggcgat cgtcgggcac aaggtggcct cgggggagta cgacctcggg
     1381 cttctgtgct gcggcaccgg ggtgggcatc tcgatggcgg ccaacaaggt gcccggtgcc
     1441 cgggcggtgg tctgctccga ggcctacacg gcaaggctgt cgcgcgagca caacgcgtcc
     1501 aacatcgtgt cggtcggtgc ccgggtggtc ggaccgggtg aggcgcggat gatcgtcgag
     1561 gcgtttctgc tcgccgagcc ggcgggcggg cggcacgccg aacgggtcaa gatgatcaat
     1621 gcgctggacg acgacgcgat cagcctggac gcgctgtacc agatgggtcc ggccgcgcat
     1681 cagtaggcgg gcggaccgtt cgaacgactg ctgagtgaca tgtcgagatc ggggtctcga
     1741 cgatcgacga ggtgccgacg ttggccggga tgacgagtcg ggcgcgtcag ctcgggtgcc
     1801 ccgggatctt ttccgcggtg aacgtggtgg tttcgacagg ctcgaccggc ggcgtcgcga
     1861 gagggcgagc ggggcatgcc gcgggcgttt ttgcgatcct gccgggcaaa cggtaatctt
     1921 gggcgttgtg ttcgggtgac cgaaccatca tgtgtgcccg ccgtgacgac tccgtccgac
     1981 gaagcgacac ccagaagcga aactgcgttc gcgagaacgt gcctctaccc acgacggaaa
     2041 gtgataccag ccggtgccca ccatccagca gttggtccgc aagggccgca ctgacaaggt
     2101 cagcaagaac aagacgcccg cgctcaaggg atccccgcag cgccgtggcg tgtgcactcg
     2161 tgtgtacacc accaccccga agaagccgaa ctccgcgctg cgcaaggtgg cgcgcgtgcg
     2221 gctcagctcc ggcgtcgagg tcaccgcgta cattccgggc gtcgggcaca acttgcagga
     2281 gcactcgatg gtgctggtcc gcggcggtcg tgtgaaggac ctgcccggcg tgcgctacaa
     2341 gatcattcgc ggctcgctcg acacccaggg tgtcaagaac cgcaagcagg ctcgcagccg
     2401 ctacggcgcg aagaaggaga agtaatgcct cgcaagggtc ccgc
//
LOCUS       DAOSUG010000480         2440 bp    DNA     linear   ENV 10-JUN-2024
DEFINITION  MAG TPA_asm: Micropruina sp. isolate AMR_MDS_2195
            NODE_17459_length_2440_cov_3.137107, whole genome shotgun sequence.
ACCESSION   DAOSUG010000480 DAOSUG010000000
VERSION     DAOSUG010000480.1
DBLINK      BioProject: PRJNA925477
            BioSample: SAMN32864262
            Sequence Read Archive: SRR4236673
KEYWORDS    WGS; ENV; Metagenome Assembled Genome; MAG; Third Party Data; TPA;
            TPA:assembly.
SOURCE      Micropruina sp. (wastewater metagenome)
  ORGANISM  Micropruina sp.
            Bacteria; Bacillati; Actinomycetota; Actinomycetes;
            Propionibacteriales; Nocardioidaceae; Micropruina.
REFERENCE   1  (bases 1 to 2440)
  AUTHORS   Abdulkadir,N., Saraiva,J.P., Zhang,J., Stolte,S., Gillor,O.,
            Harms,H. and Rocha,U.
  TITLE     Genome-centric analyses of 165 metagenomes show that mobile genetic
            elements are crucial for the transmission of antimicrobial
            resistance genes to pathogens in activated sludge and wastewater
  JOURNAL   Microbiol Spectr 12 (3), e0291823 (2024)
   PUBMED   38289113
REFERENCE   2  (bases 1 to 2440)
  AUTHORS   Abdulkadir,N.
  TITLE     Direct Submission
  JOURNAL   Submitted (24-MAR-2023) Environmental Microbiology, Helmholtz
            Center for Environmental Research -UFZ, Permoserstr 15, Leipzig
            04318, Germany
COMMENT     The annotation was added by the NCBI Prokaryotic Genome Annotation
            Pipeline (PGAP). Information about PGAP can be found here:
            https://www.ncbi.nlm.nih.gov/genome/annotation_prok/
            
            ##Genome-Assembly-Data-START##
            Assembly Method        :: SPAdes v. 3.11.1
            Genome Representation  :: Full
            Expected Final Version :: Yes
            Genome Coverage        :: Not Applicable
            Sequencing Technology  :: Illumina MiSeq
            ##Genome-Assembly-Data-END##
            
            ##Genome-Annotation-Data-START##
            Annotation Provider               :: NCBI
            Annotation Date                   :: 05/04/2023 04:57:10
            Annotation Pipeline               :: NCBI Prokaryotic Genome
                                                 Annotation Pipeline (PGAP)
            Annotation Method                 :: Best-placed reference protein
                                                 set; GeneMarkS-2+
            Annotation Software revision      :: 6.5
            Features Annotated                :: Gene; CDS; rRNA; tRNA; ncRNA
            Genes (total)                     :: 2,776
            CDSs (total)                      :: 2,750
            Genes (coding)                    :: 2,714
            CDSs (with protein)               :: 2,714
            Genes (RNA)                       :: 26
            tRNAs                             :: 24
            ncRNAs                            :: 2
            Pseudo Genes (total)              :: 36
            CDSs (without protein)            :: 36
            Pseudo Genes (ambiguous residues) :: 0 of 36
            Pseudo Genes (frameshifted)       :: 2 of 36
            Pseudo Genes (incomplete)         :: 32 of 36
            Pseudo Genes (internal stop)      :: 2 of 36
            ##Genome-Annotation-Data-END##
FEATURES             Location/Qualifiers
     source          1..2440
                     /organism="Micropruina sp."
                     /mol_type="genomic DNA"
                     /submitter_seqid="NODE_17459_length_2440_cov_3.137107"
                     /isolate="AMR_MDS_2195"
                     /isolation_source="Activated sludge of municipal
                     wastewater treatment plants"
                     /db_xref="taxon:2737536"
                     /environmental_sample
                     /geo_loc_name="Canada"
                     /lat_lon="49.25 N 123.1 W"
                     /collection_date="2015-06-16"
                     /metagenome_source="wastewater metagenome"
                     /note="metagenomic"
     gene            complement(<1..185)
                     /locus_tag="PLF56_12995"
     CDS             complement(<1..185)
                     /locus_tag="PLF56_12995"
                     /inference="COORDINATES: ab initio
                     prediction:GeneMarkS-2+"
                     /note="Derived by automated computational analysis using
                     gene prediction method: GeneMarkS-2+."
                     /codon_start=1
                     /transl_table=11
                     /product="hypothetical protein"
                     /protein_id="HOQ54514.1"
                     /translation="MRFVASSAEAAAATGVPSGDRSAEISAERTIQGAELARLAGGRI
                     VPMSDHLRAHDSEGRVSA"
     gene            complement(285..358)
                     /locus_tag="PLF56_13000"
     tRNA            complement(285..358)
                     /locus_tag="PLF56_13000"
                     /product="tRNA-Met"
                     /inference="COORDINATES: profile:tRNAscan-SE:2.0.12"
                     /note="Derived by automated computational analysis using
                     gene prediction method: tRNAscan-SE."
                     /anticodon=(pos:complement(322..324),aa:Met,seq:cat)
     gene            592..2052
                     /locus_tag="PLF56_13005"
     CDS             592..2052
                     /locus_tag="PLF56_13005"
                     /inference="COORDINATES: protein motif:HMM:NF012706.2"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="ABC transporter substrate-binding protein"
                     /protein_id="HOQ54515.1"
                     /translation="MVALLAGCVGQSPQASTDQARTLTVGATLEPPTMNPSASPAASI
                     PQALLYNVYETLVKMDAEGNLKPLLAQTWNVSPDRTEYTFRLNQAAKFASGTPVTAQA
                     VVTSFNRIKTETAIQAPLVEQMAVVKSTEAVDEHTVKVTLNHPSNLWLYDISSTAGII
                     YDPNGLATLDTTPAGSGPFVLKQWNKGQSVVLASSGSYWGTAPRLDTVTFRYFADPNA
                     MNAAMLAGDLDVISNLQAPDAIEQFSDPSRFTVIQGTTTGEVVMALNNARPALKNDKV
                     RRAISMAIDKKALLDTVWNGKGTLIGSMAVPTDPWYEDLTSINAYDPAEAKKLLAEAK
                     STNLSLKLRVPTIAYATKSAQFVESALREVGIKVTVEELDFATWLKDVHNGGNYDMSI
                     VAHVEARDLRRFTDPLYYFHYDNAEYNKQFAAADEAPSDELVPLMRKALRTLAEDAAA
                     VWLFALPNLVITKSTVTGVPQNAATLSFDLTTIATR"
     gene            2065..>2440
                     /locus_tag="PLF56_13010"
     CDS             2065..>2440
                     /locus_tag="PLF56_13010"
                     /inference="COORDINATES: protein motif:HMM:NF040248.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_process: GO:0055085 - transmembrane transport [Evidence
                     IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="ABC transporter permease"
                     /protein_id="HOQ54516.1"
                     /translation="MRILAFLGRRLGIFALSLLGASLLVFAVCAALPGEVAAIILGEG
                     ATPEQITKLTAQLGLDRPWLVRYADWVGGMLHGDFGTSYFTGQNVLQLMSPRIAVTLW
                     LVLFSLVLSVVIAVPVGMLAAMK"
BASE COUNT          430 a          859 c          764 g          387 t
ORIGIN      
        1 gcgctgacac gtccctccga gtcatgcgca cgcagatggt cgctcatggg cacaatccgg
       61 ccaccggcga gccgcgccag ctcggccccc tggatcgtgc gttcggccga aatctccgcc
      121 gatcggtcac cactgggaac cccagtggcg gcggcggctt cggcggagct ggcgacgaac
      181 cgcaccagag ccggtgagca ccaggtcggc cgccacagtc gcgcccagag agacgaaacc
      241 ggcctcgacc tggtatgtca ccttgtcgaa gccggtttca ttcgtagcgg ggacaggatt
      301 tgaacctgtg acctctgggt tatgagccca gcgagctacc gagctgctcc accccgcgtc
      361 gcctgaccaa ctataggtca acggccgggc tcacccaaat cgccttcgcg gcaaggtccg
      421 ccgagctcgt gtctcatgga cgcgccgccc cggcgagtcc ggcgggctcg gctagacttt
      481 cgccgcgttc cgctgcccaa ggcgggcagg ccacgaggag gacagatggt cttcagcacg
      541 tccgcgacgg ctgcaccgca gcgcaggtgg cggtgggctt ccctgatcgt tgtggtcgcc
      601 ttgctggccg gttgtgtcgg ccagtccccg caggcatcca ccgaccaggc ccgcacgctg
      661 acggtcggag ccacgctcga gccgccgacc atgaacccga gcgcgtcgcc ggccgcgtcc
      721 ataccgcagg cgctgctgta caacgtgtac gagaccctgg tgaaaatgga cgccgagggc
      781 aacctcaagc cgctgctcgc gcagacctgg aacgtcagcc cggatcgcac cgagtacacc
      841 tttcgtctga accaggcggc caagttcgcc agcggcacgc cggtcaccgc gcaggcggtg
      901 gtcaccagct tcaaccggat caagaccgag accgccattc aggccccact cgtggagcag
      961 atggccgtgg tcaagtccac cgaagccgtg gacgagcaca cggtcaaggt gacgctcaac
     1021 catccgtcca acctgtggct gtacgacatc agctcgaccg cgggcatcat ctacgacccg
     1081 aacggcctcg cgacgctcga caccaccccg gccggctcgg ggccgttcgt gctcaagcag
     1141 tggaacaagg gccagagcgt tgtgctcgcc tccagcggta gttactgggg taccgcgccg
     1201 cggctcgaca ccgtgacctt tcgctacttc gccgacccca acgccatgaa cgccgccatg
     1261 ctggccggcg acctggacgt gatctcgaac ctgcaggcac ccgatgccat cgagcagttc
     1321 agcgacccga gccggttcac cgtcattcag ggcaccacca ccggcgaggt ggtgatggcc
     1381 ctcaacaacg cgcggccggc gctgaagaac gacaaggtgc gccgtgcgat ctcgatggcc
     1441 atcgacaaga aggcgctgct cgacaccgtc tggaacggca agggcaccct gatcggctcc
     1501 atggcggtgc ccaccgaccc ctggtacgag gacctgacct cgatcaacgc ctacgatccc
     1561 gccgaggcca agaagctgct cgccgaggcc aagtcgacca acctgagcct caagctgcgg
     1621 gtgcccacca tcgcctacgc caccaagagc gcacagttcg tcgagagtgc gctgcgcgag
     1681 gtgggcatca aggtgaccgt cgaagagctc gacttcgcca cctggctcaa ggacgtgcac
     1741 aacggcggca actacgacat gtcgatcgtg gcgcacgtcg aggcacgcga cctgcgccgg
     1801 ttcaccgatc cgctctacta cttccactac gacaacgccg agtacaacaa gcagttcgcc
     1861 gcagcggacg aggctccgtc cgacgaactg gtgccgctga tgcgcaaggc gttgcgcacc
     1921 ctggccgagg acgccgccgc cgtctggctg ttcgcgctgc ccaatctggt gatcaccaag
     1981 tccacggtca ccggggtgcc ccagaacgcg gccacgctca gcttcgacct gaccacgatc
     2041 gccacccgat agttccggcg gccggtgcgc atcctcgcct tcctgggccg gcggctcggc
     2101 atcttcgcgc tcagcctgct gggtgcgtcc ctgctggtgt tcgccgtgtg cgcggcacta
     2161 cccggcgaag tcgcggcgat cattctgggt gagggcgcca cccccgaaca gatcacgaaa
     2221 ctgaccgccc aactcggcct ggatcgaccc tggttggtgc gctacgccga ctgggtcggt
     2281 ggcatgctgc atggcgattt cggtacctcg tacttcaccg gccagaacgt gctgcaactg
     2341 atgtcgccgc ggatcgccgt gaccctgtgg ctggtgctgt tctcgttggt gttgtcggtg
     2401 gtgatcgcgg tgccggtcgg catgctggcg gcgatgaaac
//
LOCUS       DAOSUG010000481         2419 bp    DNA     linear   ENV 10-JUN-2024
DEFINITION  MAG TPA_asm: Micropruina sp. isolate AMR_MDS_2195
            NODE_17647_length_2419_cov_4.608291, whole genome shotgun sequence.
ACCESSION   DAOSUG010000481 DAOSUG010000000
VERSION     DAOSUG010000481.1
DBLINK      BioProject: PRJNA925477
            BioSample: SAMN32864262
            Sequence Read Archive: SRR4236673
KEYWORDS    WGS; ENV; Metagenome Assembled Genome; MAG; Third Party Data; TPA;
            TPA:assembly.
SOURCE      Micropruina sp. (wastewater metagenome)
  ORGANISM  Micropruina sp.
            Bacteria; Bacillati; Actinomycetota; Actinomycetes;
            Propionibacteriales; Nocardioidaceae; Micropruina.
REFERENCE   1  (bases 1 to 2419)
  AUTHORS   Abdulkadir,N., Saraiva,J.P., Zhang,J., Stolte,S., Gillor,O.,
            Harms,H. and Rocha,U.
  TITLE     Genome-centric analyses of 165 metagenomes show that mobile genetic
            elements are crucial for the transmission of antimicrobial
            resistance genes to pathogens in activated sludge and wastewater
  JOURNAL   Microbiol Spectr 12 (3), e0291823 (2024)
   PUBMED   38289113
REFERENCE   2  (bases 1 to 2419)
  AUTHORS   Abdulkadir,N.
  TITLE     Direct Submission
  JOURNAL   Submitted (24-MAR-2023) Environmental Microbiology, Helmholtz
            Center for Environmental Research -UFZ, Permoserstr 15, Leipzig
            04318, Germany
COMMENT     The annotation was added by the NCBI Prokaryotic Genome Annotation
            Pipeline (PGAP). Information about PGAP can be found here:
            https://www.ncbi.nlm.nih.gov/genome/annotation_prok/
            
            ##Genome-Assembly-Data-START##
            Assembly Method        :: SPAdes v. 3.11.1
            Genome Representation  :: Full
            Expected Final Version :: Yes
            Genome Coverage        :: Not Applicable
            Sequencing Technology  :: Illumina MiSeq
            ##Genome-Assembly-Data-END##
            
            ##Genome-Annotation-Data-START##
            Annotation Provider               :: NCBI
            Annotation Date                   :: 05/04/2023 04:57:10
            Annotation Pipeline               :: NCBI Prokaryotic Genome
                                                 Annotation Pipeline (PGAP)
            Annotation Method                 :: Best-placed reference protein
                                                 set; GeneMarkS-2+
            Annotation Software revision      :: 6.5
            Features Annotated                :: Gene; CDS; rRNA; tRNA; ncRNA
            Genes (total)                     :: 2,776
            CDSs (total)                      :: 2,750
            Genes (coding)                    :: 2,714
            CDSs (with protein)               :: 2,714
            Genes (RNA)                       :: 26
            tRNAs                             :: 24
            ncRNAs                            :: 2
            Pseudo Genes (total)              :: 36
            CDSs (without protein)            :: 36
            Pseudo Genes (ambiguous residues) :: 0 of 36
            Pseudo Genes (frameshifted)       :: 2 of 36
            Pseudo Genes (incomplete)         :: 32 of 36
            Pseudo Genes (internal stop)      :: 2 of 36
            ##Genome-Annotation-Data-END##
FEATURES             Location/Qualifiers
     source          1..2419
                     /organism="Micropruina sp."
                     /mol_type="genomic DNA"
                     /submitter_seqid="NODE_17647_length_2419_cov_4.608291"
                     /isolate="AMR_MDS_2195"
                     /isolation_source="Activated sludge of municipal
                     wastewater treatment plants"
                     /db_xref="taxon:2737536"
                     /environmental_sample
                     /geo_loc_name="Canada"
                     /lat_lon="49.25 N 123.1 W"
                     /collection_date="2015-06-16"
                     /metagenome_source="wastewater metagenome"
                     /note="metagenomic"
     gene            184..1443
                     /locus_tag="PLF56_13015"
     CDS             184..1443
                     /locus_tag="PLF56_13015"
                     /inference="COORDINATES: protein motif:HMM:NF014567.2"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="PspC domain-containing protein"
                     /protein_id="HOQ54517.1"
                     /translation="MSASGSPVQAVPQRADRRLDSAWLAGVCSGLAAHLNWPVLVVRV
                     GFVLLSASMGLGVLVYLVLWLALPLAAEDPRAPGIDAATRGGLRTAQQRYRVPADLGT
                     MSAVVLLGAGLLWIAQSAGFGLPAWALWPAMAGAAGLMLIWWQADHIPNRGMSEVSGW
                     RRWFVPLIAHWSRIVRILLGLLGLGTAVALVMVALPDLPETTQTLIVLAMAVLALLLA
                     AAPWFVRVRRSLTSIREQKMLADARADMAAHLHDSVLQTLALIQRQAGDPKAVGALAR
                     RQERELRAWLYGETADASTVKAALTAAAAEVEDDHGVDVDIVVVGDRDLEADLRPLVQ
                     ASREAMVNAAKHAGVAKVDVYAEVDDDAVEVFVRDRGKGFVLEQIPADRMGVRGSIME
                     RLRRAGGNATIRSAPGEGTEVRLEMKR"
     gene            1440..2174
                     /locus_tag="PLF56_13020"
     CDS             1440..2174
                     /locus_tag="PLF56_13020"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_013160590.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_function: GO:0003677 - DNA binding [Evidence IEA];
                     GO_process: GO:0000160 - phosphorelay signal transduction
                     system [Evidence IEA];
                     GO_process: GO:0006355 - regulation of DNA-templated
                     transcription [Evidence IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="response regulator transcription factor"
                     /protein_id="HOQ54518.1"
                     /translation="MTEQTPAPVQGTEPQPVDPDAVGGGEQRPSRPAAHRVVIVDDHA
                     MFRSGVKHDIGGRVQIVGEGEDVATAVAAVLKAQPDVVLLDVHLPGGGGVEVLKQVHA
                     VEPEIKFLALSVSDAAEDVIGVIRGGARGYVTKSISGDELVDAIARVAEGDAVFSPRL
                     AGFVLDAFSGSIDVAAVDEELDRLSQREREVMRLIARGYAYKEIARELFISIKTVETH
                     VSSVLRKLQLSNRHQLTRWATDRRLV"
BASE COUNT          339 a          756 c          933 g          391 t
ORIGIN      
        1 ccagcgacgg gcgagtccgg cacagacgcc gccgagcacg gcgcccgagg acgcacggcg
       61 cagcggggtg agttacatgc cagccactct tgccgtcggt gctgcgccag ccaacgggga
      121 agacccctga gccgaccccc aaagcggtgg tcggccgccc cggcacgtgt gaggcttagg
      181 gacgtgtcgg cgtccggttc accggtccag gccgttccgc agcgggcgga ccggcggctg
      241 gacagtgcct ggctggccgg ggtgtgcagc gggctcgcgg cccatttgaa ctggcccgtg
      301 ctggtggtgc gggtggggtt cgtgctgctc agcgccagca tgggcctggg tgtgctggtc
      361 tacctcgtgt tgtggttggc cctgccgctg gccgccgagg atcctcgggc gccgggcatc
      421 gacgcggcga cccgaggggg actgcgcacc gcgcagcagc gctatcgggt gccggccgac
      481 ctgggcacga tgtcggccgt ggtgttgttg ggggccggcc tgctgtggat cgcgcaatcg
      541 gcgggtttcg ggctgccggc gtgggcgttg tggcccgcca tggccggtgc cgcggggttg
      601 atgctgatct ggtggcaggc cgaccacatt cccaatcgcg gcatgagcga ggtgtccggc
      661 tggcgtcggt ggttcgttcc gctgatcgcg cactggtcgc ggatcgtccg cattctgctc
      721 ggcctgctcg ggctgggtac ggccgtggcg ctggtcatgg tcgccctgcc ggatctaccg
      781 gagacaaccc agacgctgat cgtgctggcg atggcggtgc tggccctgct gttggcggcg
      841 gcgccctggt tcgtgcgggt gcgacgctcg ctgaccagca ttcgtgagca gaagatgctg
      901 gccgacgcac gcgccgacat ggccgcccac ctgcatgact cggtgttgca gacgttggcg
      961 ctgatccagc ggcaggccgg cgacccgaag gcggtggggg cgctggcgcg ccggcaggaa
     1021 cgtgaactgc gggcctggct gtacggcgag accgccgatg cctcgacggt gaaggcggcc
     1081 ctgaccgccg ccgcggccga ggtcgaggac gatcacggcg tggacgtcga catcgtcgtg
     1141 gtgggcgatc gcgacctgga ggccgacctg cgtccgctcg tccaggcgag ccgcgaggcc
     1201 atggtgaatg cggccaagca cgccggggtg gccaaggtgg acgtctatgc cgaggtcgac
     1261 gacgacgccg tcgaggtttt cgtgcgtgac cgcggcaagg gcttcgtact ggagcagatc
     1321 ccggccgacc gcatgggcgt gcgcggctcg atcatggagc gattgcggcg ggccggaggc
     1381 aatgcaacga ttcgaagtgc ccccggagag ggcaccgagg tacgactgga gatgaagcga
     1441 tgaccgagca gacaccagct cctgtgcagg gcactgagcc ccaacctgtc gacccggacg
     1501 cggtcggcgg cggcgagcag cgtccgagcc ggccggcggc gcaccgcgtc gtgatcgtgg
     1561 acgatcatgc gatgtttcgc agcggcgtca agcacgacat cggtggccgg gtgcagatcg
     1621 tcggtgaggg cgaggacgtg gccaccgccg tcgcggccgt gctgaaggcg cagccggacg
     1681 tggtgctgct cgacgtgcac ctgcccggcg gcggcggggt cgaggtgctc aagcaggtgc
     1741 atgcggtcga gcccgagatc aagtttctgg cgctcagtgt gtcggacgcg gccgaggacg
     1801 tgatcggcgt catccgcggt ggagcccggg ggtacgtgac caagtctatc tcgggggacg
     1861 aactcgtgga cgcgatcgct cgggtcgccg agggcgacgc tgtgttctcg ccccggctcg
     1921 ccggcttcgt gctggacgcc ttctcgggct cgatcgacgt ggcagcggtg gacgaagagc
     1981 tcgaccggct ctcgcagcgc gagcgcgagg tgatgcggct gatcgcccgt ggctatgcgt
     2041 acaaagagat cgcccgggag ctgttcatct cgatcaagac cgtcgagacc cacgtcagca
     2101 gcgtgttgcg caagctgcag ttgtcgaacc gtcatcagtt gaccaggtgg gccacggatc
     2161 gccggctggt gtagttggtc gagctcgtcg agacgcctgc atccgtcatc ccggcgaacg
     2221 ccgggatctt cgggttccgt catcccggcg acggccggga tctgcggccc taccggctcg
     2281 gccggctggt gagaagccct tgacgctcag accttgatga cctcgagcag gcccagttcg
     2341 gcgagcacgg cgaagtcgtc gtcctgggtg accacgggga ggccgttcgc gagcgcgacg
     2401 gaggcgatcc acaggtcgt
//
LOCUS       DAOSUG010000482         2405 bp    DNA     linear   ENV 10-JUN-2024
DEFINITION  MAG TPA_asm: Micropruina sp. isolate AMR_MDS_2195
            NODE_17780_length_2405_cov_5.122553, whole genome shotgun sequence.
ACCESSION   DAOSUG010000482 DAOSUG010000000
VERSION     DAOSUG010000482.1
DBLINK      BioProject: PRJNA925477
            BioSample: SAMN32864262
            Sequence Read Archive: SRR4236673
KEYWORDS    WGS; ENV; Metagenome Assembled Genome; MAG; Third Party Data; TPA;
            TPA:assembly.
SOURCE      Micropruina sp. (wastewater metagenome)
  ORGANISM  Micropruina sp.
            Bacteria; Bacillati; Actinomycetota; Actinomycetes;
            Propionibacteriales; Nocardioidaceae; Micropruina.
REFERENCE   1  (bases 1 to 2405)
  AUTHORS   Abdulkadir,N., Saraiva,J.P., Zhang,J., Stolte,S., Gillor,O.,
            Harms,H. and Rocha,U.
  TITLE     Genome-centric analyses of 165 metagenomes show that mobile genetic
            elements are crucial for the transmission of antimicrobial
            resistance genes to pathogens in activated sludge and wastewater
  JOURNAL   Microbiol Spectr 12 (3), e0291823 (2024)
   PUBMED   38289113
REFERENCE   2  (bases 1 to 2405)
  AUTHORS   Abdulkadir,N.
  TITLE     Direct Submission
  JOURNAL   Submitted (24-MAR-2023) Environmental Microbiology, Helmholtz
            Center for Environmental Research -UFZ, Permoserstr 15, Leipzig
            04318, Germany
COMMENT     The annotation was added by the NCBI Prokaryotic Genome Annotation
            Pipeline (PGAP). Information about PGAP can be found here:
            https://www.ncbi.nlm.nih.gov/genome/annotation_prok/
            
            ##Genome-Assembly-Data-START##
            Assembly Method        :: SPAdes v. 3.11.1
            Genome Representation  :: Full
            Expected Final Version :: Yes
            Genome Coverage        :: Not Applicable
            Sequencing Technology  :: Illumina MiSeq
            ##Genome-Assembly-Data-END##
            
            ##Genome-Annotation-Data-START##
            Annotation Provider               :: NCBI
            Annotation Date                   :: 05/04/2023 04:57:10
            Annotation Pipeline               :: NCBI Prokaryotic Genome
                                                 Annotation Pipeline (PGAP)
            Annotation Method                 :: Best-placed reference protein
                                                 set; GeneMarkS-2+
            Annotation Software revision      :: 6.5
            Features Annotated                :: Gene; CDS; rRNA; tRNA; ncRNA
            Genes (total)                     :: 2,776
            CDSs (total)                      :: 2,750
            Genes (coding)                    :: 2,714
            CDSs (with protein)               :: 2,714
            Genes (RNA)                       :: 26
            tRNAs                             :: 24
            ncRNAs                            :: 2
            Pseudo Genes (total)              :: 36
            CDSs (without protein)            :: 36
            Pseudo Genes (ambiguous residues) :: 0 of 36
            Pseudo Genes (frameshifted)       :: 2 of 36
            Pseudo Genes (incomplete)         :: 32 of 36
            Pseudo Genes (internal stop)      :: 2 of 36
            ##Genome-Annotation-Data-END##
FEATURES             Location/Qualifiers
     source          1..2405
                     /organism="Micropruina sp."
                     /mol_type="genomic DNA"
                     /submitter_seqid="NODE_17780_length_2405_cov_5.122553"
                     /isolate="AMR_MDS_2195"
                     /isolation_source="Activated sludge of municipal
                     wastewater treatment plants"
                     /db_xref="taxon:2737536"
                     /environmental_sample
                     /geo_loc_name="Canada"
                     /lat_lon="49.25 N 123.1 W"
                     /collection_date="2015-06-16"
                     /metagenome_source="wastewater metagenome"
                     /note="metagenomic"
     gene            complement(<1..503)
                     /locus_tag="PLF56_13025"
     CDS             complement(<1..503)
                     /locus_tag="PLF56_13025"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_012921008.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="thioesterase family protein"
                     /protein_id="HOQ54519.1"
                     /translation="MPRTTDELLGLFRLEQLDTDLFRGPQPETSLQRSFGGQVLAQGL
                     AAAYRTVPNDRLAHSLNAYFLRPGSPATPILYVVEITRDGRTFSQRRIVARQDGRAIF
                     SMVCSFHVHEEGLDHADPLPGDLPPPEDCRPAAVVLGERSARRAAFWQMEWGALDVRY
                     VREPSQAG"
     gene            complement(507..1406)
                     /locus_tag="PLF56_13030"
     CDS             complement(507..1406)
                     /locus_tag="PLF56_13030"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_013863072.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_process: GO:0043248 - proteasome assembly [Evidence
                     IEA];
                     GO_process: GO:0051131 - chaperone-mediated protein
                     complex assembly [Evidence IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="PAC2 family protein"
                     /protein_id="HOQ54520.1"
                     /translation="MLDPRLLYTFNPEVWASLDGARPVLIHLFEGYVDAAAVTRNLSG
                     HILATCEHEVLAEFDHDQLHDYRSRRPQMVFDTNKWVSMTDFSLLLHKVTDPDGTPFL
                     LLTGPEPDTQWRRMAQAVLGIAERLDSALLLTASGVPMAVPHTRPTLVTSHSTDPERA
                     GGNPLWIDRIQIPGSFSAVLEYRGGHTGLTGLGFVAHVPHYLAQATFPQATAAVLRRM
                     NDAAGLSIPVGALDDEADQQLATIAAEVHDDSDFPAVLSALEEQYDQLAESGRAAVPT
                     ADEIGAAVEQFLAEQDDPNDGRG"
     gene            complement(1532..2170)
                     /locus_tag="PLF56_13035"
     CDS             complement(1532..2170)
                     /locus_tag="PLF56_13035"
                     /inference="COORDINATES: protein motif:HMM:NF014906.2"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_process: GO:0045892 - negative regulation of
                     DNA-templated transcription [Evidence IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="TetR/AcrR family transcriptional regulator
                     C-terminal domain-containing protein"
                     /protein_id="HOQ54521.1"
                     /translation="MSAARGSLSTGVIVGAAFRVLERSGLSGVTARAVAAELGVRPSA
                     LYHHLPDMATLLDEMATAMRREMNAQPSPRWDATLRETGRTVRRVLLTHRDGGRLFAG
                     RRLRDPSLLPAMEEPLRTLTSAGLSLELAVGALQSVLDLTVGFVIEQQHRASGESGEY
                     DPTARQSLVPPDRFPLTSAASGPLFDDPDVRYEIALGLLVDGVAAHVPPTPA"
BASE COUNT          393 a          795 c          853 g          364 t
ORIGIN      
        1 ccggcctgcg acggctcccg cacgtagcgg acgtccaggg caccccattc catctgccag
       61 aaggcggcac gccgggccga ccgctccccc agcaccacgg cggctggtcg gcagtcctcc
      121 ggtggcggca ggtcgccggg cagcgggtcg gcatggtcga ggccctcttc gtgcacgtgg
      181 aacgagcaga ccatcgagaa gatcgcacgg ccgtcctgcc tggccacaat gcggcgttga
      241 ctgaaggtac gcccgtcacg ggtgatctcg accacgtaca ggatcggcgt ggcgggcgac
      301 ccgggtcgca gaaagtaggc gttcagcgag tgcgcgagcc gatcgttggg aacggtgcgg
      361 taggccgcgg ccaacccctg ggccaacacc tggcccccga acgatcgctg cagcgaggtc
      421 tcgggctgcg ggccgcggaa caggtcggtg tccagttgct ccaggcgaaa caggcccaga
      481 agctcgtccg tggtgcgggg cacggctcag cctcggccgt cgttcggatc gtcctgctcg
      541 gcgaggaact gctcgaccgc ggccccgatc tcatcggcgg tcgggacggc ggcgcgtccg
      601 gactctgcca actggtcgta ctgctcttcg agggccgaca gcacggcggg aaagtcggaa
      661 tcgtcgtgta cttcagccgc gatggtggcg agttgctggt cggcctcatc gtcgagcgcg
      721 ccgacgggaa tgctcaggcc ggcagcgtcg ttcatgcgcc gcaacaccgc tgccgtcgcc
      781 tgcggaaagg tggcctgcgc caggtagtgc ggcacgtggg cgacgaatcc caggccggtc
      841 agtccggtgt ggccgccgcg gtactcgagc acggccgaga agctgccggg gatctggatg
      901 cggtcgatcc acaacgggtt gcccccggcg cgttcgggat cggtcgaatg cgaggtgacc
      961 agcgtcggtc gcgtgtgcgg caccgccatc ggcaccccac tggcggtgag cagcagcgcc
     1021 gaatcgaggc gctcggcgat gcccagcacg gcctgcgcca tgcgccgcca ctgggtgtcg
     1081 ggctcggggc cggtgagcag caggaacggg gtgccgtcgg ggtcggtgac cttgtgcagc
     1141 agcagcgaga agtccgtcat cgagacccac ttgttggtgt cgaagaccat ctgcggacgc
     1201 ctgctgcggt agtcgtgcag ttgatcgtga tcgaactcgg cgagcacctc gtgctcgcag
     1261 gtggccagta tgtgaccgga caggttgcgg gtgaccgcgg ccgcgtcgac gtaaccctcg
     1321 aacaggtgga tcagcaccgg gcgagcaccg tccaggctgg cccacacctc ggggttgaac
     1381 gtgtacagca accgtggatc gagcacgtcg gcaaccctac cgtgcggcct cggcggtccc
     1441 gaggctctac gccgtcggcg tagggcggcc aacgcccgcc taactttcca gggcctgcgc
     1501 aactccgcgg agttgcgcag gccctggaaa gttaggcggg ggttgggggt acgtgggctg
     1561 cgaccccgtc caccagcagt ccgagcgcga tctcgtagcg gacgtccggg tcgtcgaaca
     1621 gcggaccact ggcggccgag gtgagcggaa agcgatccgg tggcaccagc gactggcgcg
     1681 ccgtcgggtc gtactcgccg gactcacccg aggcgcggtg ctgctgctcg atcacgaaac
     1741 cgacggtcag atcgagcacc gactgcaggg cgcccacggc cagttccagc gaaagacccg
     1801 ccgaggtcag cgtgcgcagt ggttcctcca tggcgggtag cagggacggg tcgcgcaacc
     1861 gtcgtccggc gaacagtcgt ccgccgtcgc gatgggtgag caggacccgg cgcacggtgc
     1921 gtccggtctc gcgcagcgtg gcgtcccagc gtggcgaggg ttgggcgttc atctcacgac
     1981 gcatcgcggt cgccatctcg tcgagcagcg tcgccatgtc gggcaggtgg tgatacaggg
     2041 cgctggggcg aacgccgagt tcggcggcca ctgcccgcgc cgtcacaccg ctcaatccgg
     2101 accgttcgag cacgcgaaac gccgcaccga cgatcacgcc ggtactcaaa ctgcctcgcg
     2161 cagcgctcac ggcacctccg gttgacttct gaactttgtt cactaaccta gcaggtgaac
     2221 aatgttcagg aggcatcatg aaagcagcaa tcgtcaccga cttcgacact gcaccgcagt
     2281 acggcaccct cgccgacccg gtcgccgatc agggataccg ggtggtcacc gtgctggcag
     2341 cgggcgttca tcaactcgtg cgcagcaggg cgtccggccg gcactacagc agcaccgatg
     2401 tgctg
//
LOCUS       DAOSUG010000483         2404 bp    DNA     linear   ENV 10-JUN-2024
DEFINITION  MAG TPA_asm: Micropruina sp. isolate AMR_MDS_2195
            NODE_17793_length_2404_cov_4.100043, whole genome shotgun sequence.
ACCESSION   DAOSUG010000483 DAOSUG010000000
VERSION     DAOSUG010000483.1
DBLINK      BioProject: PRJNA925477
            BioSample: SAMN32864262
            Sequence Read Archive: SRR4236673
KEYWORDS    WGS; ENV; Metagenome Assembled Genome; MAG; Third Party Data; TPA;
            TPA:assembly.
SOURCE      Micropruina sp. (wastewater metagenome)
  ORGANISM  Micropruina sp.
            Bacteria; Bacillati; Actinomycetota; Actinomycetes;
            Propionibacteriales; Nocardioidaceae; Micropruina.
REFERENCE   1  (bases 1 to 2404)
  AUTHORS   Abdulkadir,N., Saraiva,J.P., Zhang,J., Stolte,S., Gillor,O.,
            Harms,H. and Rocha,U.
  TITLE     Genome-centric analyses of 165 metagenomes show that mobile genetic
            elements are crucial for the transmission of antimicrobial
            resistance genes to pathogens in activated sludge and wastewater
  JOURNAL   Microbiol Spectr 12 (3), e0291823 (2024)
   PUBMED   38289113
REFERENCE   2  (bases 1 to 2404)
  AUTHORS   Abdulkadir,N.
  TITLE     Direct Submission
  JOURNAL   Submitted (24-MAR-2023) Environmental Microbiology, Helmholtz
            Center for Environmental Research -UFZ, Permoserstr 15, Leipzig
            04318, Germany
COMMENT     The annotation was added by the NCBI Prokaryotic Genome Annotation
            Pipeline (PGAP). Information about PGAP can be found here:
            https://www.ncbi.nlm.nih.gov/genome/annotation_prok/
            
            ##Genome-Assembly-Data-START##
            Assembly Method        :: SPAdes v. 3.11.1
            Genome Representation  :: Full
            Expected Final Version :: Yes
            Genome Coverage        :: Not Applicable
            Sequencing Technology  :: Illumina MiSeq
            ##Genome-Assembly-Data-END##
            
            ##Genome-Annotation-Data-START##
            Annotation Provider               :: NCBI
            Annotation Date                   :: 05/04/2023 04:57:10
            Annotation Pipeline               :: NCBI Prokaryotic Genome
                                                 Annotation Pipeline (PGAP)
            Annotation Method                 :: Best-placed reference protein
                                                 set; GeneMarkS-2+
            Annotation Software revision      :: 6.5
            Features Annotated                :: Gene; CDS; rRNA; tRNA; ncRNA
            Genes (total)                     :: 2,776
            CDSs (total)                      :: 2,750
            Genes (coding)                    :: 2,714
            CDSs (with protein)               :: 2,714
            Genes (RNA)                       :: 26
            tRNAs                             :: 24
            ncRNAs                            :: 2
            Pseudo Genes (total)              :: 36
            CDSs (without protein)            :: 36
            Pseudo Genes (ambiguous residues) :: 0 of 36
            Pseudo Genes (frameshifted)       :: 2 of 36
            Pseudo Genes (incomplete)         :: 32 of 36
            Pseudo Genes (internal stop)      :: 2 of 36
            ##Genome-Annotation-Data-END##
FEATURES             Location/Qualifiers
     source          1..2404
                     /organism="Micropruina sp."
                     /mol_type="genomic DNA"
                     /submitter_seqid="NODE_17793_length_2404_cov_4.100043"
                     /isolate="AMR_MDS_2195"
                     /isolation_source="Activated sludge of municipal
                     wastewater treatment plants"
                     /db_xref="taxon:2737536"
                     /environmental_sample
                     /geo_loc_name="Canada"
                     /lat_lon="49.25 N 123.1 W"
                     /collection_date="2015-06-16"
                     /metagenome_source="wastewater metagenome"
                     /note="metagenomic"
     gene            <1..310
                     /locus_tag="PLF56_13040"
     CDS             <1..310
                     /locus_tag="PLF56_13040"
                     /inference="COORDINATES: protein motif:HMM:NF016895.2"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_component: GO:0016020 - membrane [Evidence IEA];
                     GO_function: GO:0042834 - peptidoglycan binding [Evidence
                     IEA]"
                     /codon_start=2
                     /transl_table=11
                     /product="SPOR domain-containing protein"
                     /protein_id="HOQ54522.1"
                     /translation="VSPQAQPSTSSSAVPFKKGDWLLVLASLPKKTKSQDDARAVASQ
                     LNKKSATKVLDGAAVPSLKDEYWYVVAKTAFKSRSAATKACDKFGRKAGDECNPRQVM
                     "
     gene            complement(315..1205)
                     /locus_tag="PLF56_13045"
     CDS             complement(315..1205)
                     /locus_tag="PLF56_13045"
                     /inference="COORDINATES: protein motif:HMM:TIGR01297.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_function: GO:0008324 - monoatomic cation transmembrane
                     transporter activity [Evidence IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="cation diffusion facilitator family transporter"
                     /protein_id="HOQ54523.1"
                     /translation="MTPTSAPPNENRLLERFLWLSVATAVATVTLKVIAAMITNSVGM
                     WSDALESTVNLLAAGVALWALRVSARPADHNHDFGHGKAEYLSAAVEGTLIIVAAAAI
                     IAGAVQRLLNPVPLESVGLGVALATIASAFNLATGLVLIRAGKRYRSITLEADGRHLM
                     ADVWTSVGVLVGITVVGLTHIYWLDPVIALAVGVNIIFTGYGLVRRSVVGLLDATLPP
                     EEVALIQKALGPLTDDPRVRVTDLRTRESGRQRFVQATLTVPGEWTVQRSHDLADRVE
                     AEVGQVLPGTVTFVHVEPAD"
     gene            complement(1214..1819)
                     /locus_tag="PLF56_13050"
     CDS             complement(1214..1819)
                     /locus_tag="PLF56_13050"
                     /inference="COORDINATES: protein motif:HMM:NF012514.2"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_function: GO:0016787 - hydrolase activity [Evidence
                     IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="NUDIX domain-containing protein"
                     /protein_id="HOQ54524.1"
                     /translation="MSEPFRTRTFVAVPPQERPVRTRSAVRVIVTDGSSVLLLSDTDP
                     GVAGSRWWVTPGGGIDAGEQPLQAAVRELAEETGRTVATDELVGPVMRRLVIHGYSDQ
                     VLAQTELFYLLTVAEPFELDVSGFTAEEKLTIKAWDWLPIERLQLITDPVWPGDLAEL
                     IALGVHGGEWPREAGVVEESTVNVGALGERIALDWPGFREP"
     gene            complement(1819..>2404)
                     /locus_tag="PLF56_13055"
     CDS             complement(1819..>2404)
                     /locus_tag="PLF56_13055"
                     /inference="COORDINATES: protein motif:HMM:NF023043.2"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=2
                     /transl_table=11
                     /product="DUF4352 domain-containing protein"
                     /protein_id="HOQ54525.1"
                     /translation="GAVIVLLIVGLLGWPLLSPLLSGQPTPSPSPSPVSTAPSAAPSA
                     TGSPQPTVVRTSAAARPTTSTPVTGGGIGQSTSFTTEGGSGKVTVTTASWTDNGILDP
                     QPGSAYLIVDLTFEGVSGAISTGPFFTSVTDADDETYLMSIGADLPRQLTMRTLRAGE
                     KNTGQVAFELPKGPVTFTVRSELLEPLATIQIPG"
BASE COUNT          402 a          799 c          823 g          380 t
ORIGIN      
        1 ggtatcgcct caggctcagc caagcacctc gtccagtgca gtgcccttca agaagggaga
       61 ttggctgctc gtgctggcgt cccttccgaa gaaaaccaag agtcaagacg acgcacgagc
      121 cgtcgccagc cagctcaaca agaagagcgc taccaaagtg ctggatgggg cagcggtccc
      181 gagtctcaag gatgagtatt ggtacgtggt tgccaagacc gcgttcaaga gtcgatccgc
      241 agcgaccaag gcctgcgaca agttcgggcg caaagccggg gacgagtgca atccccgcca
      301 agtgatgtag ccgatcagtc cgccggttcc acgtgcacga acgtgaccgt gcccggcagc
      361 acctgcccga cctcggcctc gacccggtcg gccagatcgt gactgcgctg cacggtccat
      421 tcgccgggca cggtgagggt ggcctgcacg aaccgttgac ggcctgattc acgggtgcgc
      481 aggtcggtca cccgcacccg cggatcgtcc gtcagtggac cgagcgcctt ctggatcaag
      541 gccacttcct cgggcggcag ggtcgcatcg agcaggccta ccaccgaacg acgcaccagt
      601 ccgtacccgg tgaagatgat gttgacaccc accgcgagcg cgatcaccgg gtcgagccag
      661 tagatgtggg tgagccctac caccgtgatg ccgaccagca cgccgaccga ggtccacaca
      721 tcggccatca ggtgccggcc gtcggcttcc agtgtgatgg agcggtaccg cttgccggcc
      781 cggatcagca ccagcccggt ggccaggttg aacgcggacg cgatggtggc cagcgcgacg
      841 ccgagcccca cgctttcgag cggcacgggg ttcagcaggc gctgaaccgc gcccgcgatg
      901 atggctgcgg cggccacgat gatcagggtt ccctcgaccg ctgccgacag gtattcggcc
      961 ttgccgtggc cgaaatcatg attgtggtcg gccggccgcg ccgagacgcg aagggcccac
     1021 agggccacgc ctgccgccag caggttgacc gtcgattcca gcgcgtcgga ccacatgccg
     1081 accgagttgg tgatcatcgc ggcgatcacc ttgagcgtga ccgttgccac tgcggtggcc
     1141 accgagagcc acaggaagcg ttccagaagc cggttttcgt tcggtggggc ggaggtggga
     1201 gtcatcggct ctttcagggt tcgcggaagc cgggccagtc tagggcgatg cgttcgccca
     1261 gcgcaccgac gttcacagtg gactcctcga ccacgccggc ctcgcgcggc cactcgccgc
     1321 cgtggacgcc cagcgcgatc agttcggcca ggtcgccggg ccacaccggg tcggtgatca
     1381 gctgcaggcg ctcgatcggc aaccagtccc atgccttgat cgtgagcttc tcctcggcgg
     1441 tgaagccgga cacgtccagt tcgaacggct cggcgaccgt cagcaggtag aacaactcgg
     1501 tctgggccaa tacctgatcg ctgtagccgt gaatcaccag gcgtcgcatc accgggccga
     1561 cgagttcgtc ggtcgcgacc gtgcgtccgg tctcttcggc caactcgcgc accgccgcct
     1621 gcagcggctg ttcgcccgcg tcgatacctc caccgggggt cacccaccag cgggagccgg
     1681 ccacgcctgg gtcggtgtcg ctcagcagga gcacgctgct gccgtcggtg acgatcaccc
     1741 gtacggctga ccgggtgcgc accggacgtt cctgcggcgg caccgcgacg aacgtgcggg
     1801 tgcggaacgg ctcggacatc agcccggaat ctggatcgtg gccagcggct ccagcagttc
     1861 gcttcgcacg gtgaacgtga ccgggccctt gggcagttcg aacgccacct ggccggtgtt
     1921 cttctcgccg gcacgcaggg tgcgcatggt cagttgccgc ggcaggtcgg ccccgatgct
     1981 catcaggtac gtttcgtcgt cggcatcggt caccgaggtg aagaacgggc cggtgctgat
     2041 ggcgccggac actccctcaa acgtgaggtc cacgatcagg taggccgaac cgggttgggg
     2101 gtcgagaatg ccgttgtcgg tccaggaggc cgtggtcacg gtgacctttc cggagccgcc
     2161 ttcggtggtg aatgaggtgg actgcccgat gccgcccccg gtcaccggcg tactggtggt
     2221 cggacgggct gccgcactgg tgcgcaccac cgtcggctgc gggctgcccg tcgcactggg
     2281 cgcggcggac ggtgccgtgc tcaccgggct cggggacggg ctgggtgtcg gttggccgga
     2341 cagcagcggc gagagcagtg gccagcccag caggccgacg atcagcagca cgatgaccgc
     2401 accg
//
LOCUS       DAOSUG010000484         2399 bp    DNA     linear   ENV 10-JUN-2024
DEFINITION  MAG TPA_asm: Micropruina sp. isolate AMR_MDS_2195
            NODE_17840_length_2399_cov_3.695819, whole genome shotgun sequence.
ACCESSION   DAOSUG010000484 DAOSUG010000000
VERSION     DAOSUG010000484.1
DBLINK      BioProject: PRJNA925477
            BioSample: SAMN32864262
            Sequence Read Archive: SRR4236673
KEYWORDS    WGS; ENV; Metagenome Assembled Genome; MAG; Third Party Data; TPA;
            TPA:assembly.
SOURCE      Micropruina sp. (wastewater metagenome)
  ORGANISM  Micropruina sp.
            Bacteria; Bacillati; Actinomycetota; Actinomycetes;
            Propionibacteriales; Nocardioidaceae; Micropruina.
REFERENCE   1  (bases 1 to 2399)
  AUTHORS   Abdulkadir,N., Saraiva,J.P., Zhang,J., Stolte,S., Gillor,O.,
            Harms,H. and Rocha,U.
  TITLE     Genome-centric analyses of 165 metagenomes show that mobile genetic
            elements are crucial for the transmission of antimicrobial
            resistance genes to pathogens in activated sludge and wastewater
  JOURNAL   Microbiol Spectr 12 (3), e0291823 (2024)
   PUBMED   38289113
REFERENCE   2  (bases 1 to 2399)
  AUTHORS   Abdulkadir,N.
  TITLE     Direct Submission
  JOURNAL   Submitted (24-MAR-2023) Environmental Microbiology, Helmholtz
            Center for Environmental Research -UFZ, Permoserstr 15, Leipzig
            04318, Germany
COMMENT     The annotation was added by the NCBI Prokaryotic Genome Annotation
            Pipeline (PGAP). Information about PGAP can be found here:
            https://www.ncbi.nlm.nih.gov/genome/annotation_prok/
            
            ##Genome-Assembly-Data-START##
            Assembly Method        :: SPAdes v. 3.11.1
            Genome Representation  :: Full
            Expected Final Version :: Yes
            Genome Coverage        :: Not Applicable
            Sequencing Technology  :: Illumina MiSeq
            ##Genome-Assembly-Data-END##
            
            ##Genome-Annotation-Data-START##
            Annotation Provider               :: NCBI
            Annotation Date                   :: 05/04/2023 04:57:10
            Annotation Pipeline               :: NCBI Prokaryotic Genome
                                                 Annotation Pipeline (PGAP)
            Annotation Method                 :: Best-placed reference protein
                                                 set; GeneMarkS-2+
            Annotation Software revision      :: 6.5
            Features Annotated                :: Gene; CDS; rRNA; tRNA; ncRNA
            Genes (total)                     :: 2,776
            CDSs (total)                      :: 2,750
            Genes (coding)                    :: 2,714
            CDSs (with protein)               :: 2,714
            Genes (RNA)                       :: 26
            tRNAs                             :: 24
            ncRNAs                            :: 2
            Pseudo Genes (total)              :: 36
            CDSs (without protein)            :: 36
            Pseudo Genes (ambiguous residues) :: 0 of 36
            Pseudo Genes (frameshifted)       :: 2 of 36
            Pseudo Genes (incomplete)         :: 32 of 36
            Pseudo Genes (internal stop)      :: 2 of 36
            ##Genome-Annotation-Data-END##
FEATURES             Location/Qualifiers
     source          1..2399
                     /organism="Micropruina sp."
                     /mol_type="genomic DNA"
                     /submitter_seqid="NODE_17840_length_2399_cov_3.695819"
                     /isolate="AMR_MDS_2195"
                     /isolation_source="Activated sludge of municipal
                     wastewater treatment plants"
                     /db_xref="taxon:2737536"
                     /environmental_sample
                     /geo_loc_name="Canada"
                     /lat_lon="49.25 N 123.1 W"
                     /collection_date="2015-06-16"
                     /metagenome_source="wastewater metagenome"
                     /note="metagenomic"
     gene            417..1781
                     /locus_tag="PLF56_13060"
     CDS             417..1781
                     /locus_tag="PLF56_13060"
                     /EC_number="4.1.99.3"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_008361933.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_function: GO:0003677 - DNA binding [Evidence IEA];
                     GO_function: GO:0003904 - deoxyribodipyrimidine
                     photo-lyase activity [Evidence IEA];
                     GO_function: GO:0050660 - flavin adenine dinucleotide
                     binding [Evidence IEA];
                     GO_process: GO:0006281 - DNA repair [Evidence IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="deoxyribodipyrimidine photo-lyase"
                     /protein_id="HOQ54526.1"
                     /translation="MTAILWLRRDLRRADHPALAKAHEGGAVLPLFVLDPGIFDSAGP
                     VRQAWLSRTLRALDQAYDGRLCIRHGDPASVLPQVAAELGADSVHVSTETEPDGASRD
                     RRVREALERAGVAWVETGSPYAVTPGRIRNQQGAPYKVFTPFSRAWREHGWRAPSEEP
                     RGLRLAKAASDDAAWQQLDRAATAQGLPELPDAGEVTAMRRWHEFVENGLPGYAAGRD
                     RPDVDGTSRLSPYLKFGVVHPRTLLADLAGRSAKGADRYGTELAWREFYADVLHHNPD
                     SLWHDLRPELKGMPYDDDADLIEAWRRGRTGFPFVDAGMRQLLAIGWMHNRVRMVTAS
                     FLCKDLHVWWPVGAQHFLDHLIDGDLASNNHGWQWVAGTGTDASPYFRVFNPVGQGRK
                     FDPDGAYVRQWVPELRHVDGAAVHEPWTQADGYAHDYPARIIDHAEQRRVALDRLALA
                     KADR"
     gene            1916..2296
                     /locus_tag="PLF56_13065"
     CDS             1916..2296
                     /locus_tag="PLF56_13065"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_014846656.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="DUF948 domain-containing protein"
                     /protein_id="HOQ54527.1"
                     /translation="MSVGEIAGLIAAIAAVALVAFCAVPLLKLGRVLEELRLVVRDVG
                     HSSVPILHELKGTVTATNDELGKLSLVTEDVSKVSRHAVVVSEDAAQLTTLFSATLGG
                     PLVKTAAFTHGVRQAIKGTRKVKS"
BASE COUNT          354 a          836 c          846 g          363 t
ORIGIN      
        1 atgcgttgac caaccgggag ggctggcccg tgctcgcgca ggagtggcgg cccgcctcgg
       61 tcgcgctgcc ggcgtacgtg tggcacggct gggacgatct ggactccggc gacgaccagc
      121 aggcgtgaac gtgtgcagcg gacggggcgg cgcggtagcc aggaccaatc gccggagcgt
      181 gcatttgatc gcgcttcccc ccggccccgg atcgtgaaca ccccggcccc gttgcgggcc
      241 tgattgcgag cgggtgggtt ttggcaccgc agtcggtgtc aaaacccacc cgctccgcac
      301 agcgctgctt tccagcccgg tgcgtcacgc gacgatgtgc ccggcgtggg tgatcgcagg
      361 gcggacgacg aggcggcgct cggtggatgt gggccaaccg tgtcacgatg cgggtcatga
      421 cagcgattct gtggctgcgt cgtgacctgc gacgagccga ccatccggcc ctggcgaaag
      481 cacatgaggg cggtgccgtg ctgccgctgt tcgtgctcga cccgggcatc ttcgattcgg
      541 ccggaccggt gcggcaggcc tggctgtccc gtactttgcg cgccctggac caggcctacg
      601 acggacgact ctgcattcgg cacggcgacc cggcgtccgt gctaccccag gtggccgccg
      661 agctgggggc ggacagcgtc catgtgtcga ccgagaccga gccggacggc gcctcccgtg
      721 accgtcgggt tcgcgaggcg ctggagcggg ccggcgtggc ctgggtcgag accggcagcc
      781 cctacgccgt cacccccggg cgcatccgca accagcaggg agcgccctac aaggtgttca
      841 ccccgttctc gcgggcctgg cgtgagcacg gctggcgcgc gccgtccgag gaaccgcgcg
      901 ggctgcggct ggcgaaggcc gccagcgacg acgccgcgtg gcaacaactc gatcgggccg
      961 ctaccgcgca gggcctgccc gagttgcccg acgcgggcga ggtcacggcc atgcgccgct
     1021 ggcacgagtt cgtggagaac ggcctgcccg gctacgccgc cggccgcgac cggcccgatg
     1081 tggacggtac gtccaggctg tcgccgtacc tcaagttcgg ggtcgtccac ccgcgcacgc
     1141 tgctcgccga cctggccgga cgctccgcca agggcgcaga ccgctacggc accgagttgg
     1201 cctggcgcga gttctacgcc gacgtgctgc accacaaccc ggattcgctg tggcacgacc
     1261 tgcgccccga gctcaaaggc atgccctacg acgacgacgc cgatctgatc gaggcctggc
     1321 ggcggggccg caccggcttt ccgttcgtgg acgcgggcat gcggcagttg ctggctatcg
     1381 gctggatgca caaccgcgtc cgcatggtga cggccagctt tctgtgcaag gacctgcacg
     1441 tgtggtggcc ggtgggtgct cagcattttc tcgaccacct gatcgacggc gacctggcct
     1501 ccaacaatca cggctggcag tgggtggccg gcaccggcac cgacgcgtcg ccgtacttcc
     1561 gtgtcttcaa cccggtcggg cagggtcgga agttcgatcc ggacggcgcc tacgtgcggc
     1621 aatgggtgcc cgaactgcgc catgtggacg gggccgccgt ccacgaaccc tggacgcagg
     1681 cggacgggta tgcccacgac tacccggccc ggatcatcga tcacgccgag caacgccgcg
     1741 tcgctctgga ccggctggcc ctggccaagg ccgaccggtg accgcgacga ccacgccggg
     1801 tgacgccacc caccgtcggc cggacggcgc cgcgcatagc ttcgtgggcg tccaaccggc
     1861 cgggccggtg cgttaggctg cgacagtcac cgtttcgagt cgaaggaacc cccaaatgtc
     1921 cgttggagag atcgccggcc tgatcgcggc catcgccgcc gtcgcactgg tcgccttctg
     1981 tgcggttccg ctgctcaagc tgggccgggt gctagaggag ctgcggctcg tcgtgcgcga
     2041 tgtcggccac agctcggtgc cgatcctgca cgagctcaag ggcacggtca ccgccaccaa
     2101 tgacgaactg ggcaagctca gcctggtcac cgaagacgtc tccaaggtca gccggcacgc
     2161 cgtcgtggtc agcgaggacg ccgcgcagct cacgaccctg ttctcggcca ccctgggtgg
     2221 cccgttggtg aagaccgccg cgttcaccca cggggtgcgc caggccatca agggcacccg
     2281 aaaggtcaag agctgatgcg ccgccgactg ttctggttcg tcgtcggcgt cgggctcacc
     2341 gcgttcgtgg tggtcaaggg ccgcgagtac tacgagaagt tcacgcccaa gggcatcac
//
LOCUS       DAOSUG010000485         2383 bp    DNA     linear   ENV 10-JUN-2024
DEFINITION  MAG TPA_asm: Micropruina sp. isolate AMR_MDS_2195
            NODE_18010_length_2383_cov_5.026203, whole genome shotgun sequence.
ACCESSION   DAOSUG010000485 DAOSUG010000000
VERSION     DAOSUG010000485.1
DBLINK      BioProject: PRJNA925477
            BioSample: SAMN32864262
            Sequence Read Archive: SRR4236673
KEYWORDS    WGS; ENV; Metagenome Assembled Genome; MAG; Third Party Data; TPA;
            TPA:assembly.
SOURCE      Micropruina sp. (wastewater metagenome)
  ORGANISM  Micropruina sp.
            Bacteria; Bacillati; Actinomycetota; Actinomycetes;
            Propionibacteriales; Nocardioidaceae; Micropruina.
REFERENCE   1  (bases 1 to 2383)
  AUTHORS   Abdulkadir,N., Saraiva,J.P., Zhang,J., Stolte,S., Gillor,O.,
            Harms,H. and Rocha,U.
  TITLE     Genome-centric analyses of 165 metagenomes show that mobile genetic
            elements are crucial for the transmission of antimicrobial
            resistance genes to pathogens in activated sludge and wastewater
  JOURNAL   Microbiol Spectr 12 (3), e0291823 (2024)
   PUBMED   38289113
REFERENCE   2  (bases 1 to 2383)
  AUTHORS   Abdulkadir,N.
  TITLE     Direct Submission
  JOURNAL   Submitted (24-MAR-2023) Environmental Microbiology, Helmholtz
            Center for Environmental Research -UFZ, Permoserstr 15, Leipzig
            04318, Germany
COMMENT     The annotation was added by the NCBI Prokaryotic Genome Annotation
            Pipeline (PGAP). Information about PGAP can be found here:
            https://www.ncbi.nlm.nih.gov/genome/annotation_prok/
            
            ##Genome-Assembly-Data-START##
            Assembly Method        :: SPAdes v. 3.11.1
            Genome Representation  :: Full
            Expected Final Version :: Yes
            Genome Coverage        :: Not Applicable
            Sequencing Technology  :: Illumina MiSeq
            ##Genome-Assembly-Data-END##
            
            ##Genome-Annotation-Data-START##
            Annotation Provider               :: NCBI
            Annotation Date                   :: 05/04/2023 04:57:10
            Annotation Pipeline               :: NCBI Prokaryotic Genome
                                                 Annotation Pipeline (PGAP)
            Annotation Method                 :: Best-placed reference protein
                                                 set; GeneMarkS-2+
            Annotation Software revision      :: 6.5
            Features Annotated                :: Gene; CDS; rRNA; tRNA; ncRNA
            Genes (total)                     :: 2,776
            CDSs (total)                      :: 2,750
            Genes (coding)                    :: 2,714
            CDSs (with protein)               :: 2,714
            Genes (RNA)                       :: 26
            tRNAs                             :: 24
            ncRNAs                            :: 2
            Pseudo Genes (total)              :: 36
            CDSs (without protein)            :: 36
            Pseudo Genes (ambiguous residues) :: 0 of 36
            Pseudo Genes (frameshifted)       :: 2 of 36
            Pseudo Genes (incomplete)         :: 32 of 36
            Pseudo Genes (internal stop)      :: 2 of 36
            ##Genome-Annotation-Data-END##
FEATURES             Location/Qualifiers
     source          1..2383
                     /organism="Micropruina sp."
                     /mol_type="genomic DNA"
                     /submitter_seqid="NODE_18010_length_2383_cov_5.026203"
                     /isolate="AMR_MDS_2195"
                     /isolation_source="Activated sludge of municipal
                     wastewater treatment plants"
                     /db_xref="taxon:2737536"
                     /environmental_sample
                     /geo_loc_name="Canada"
                     /lat_lon="49.25 N 123.1 W"
                     /collection_date="2015-06-16"
                     /metagenome_source="wastewater metagenome"
                     /note="metagenomic"
     gene            <1..756
                     /locus_tag="PLF56_13070"
     CDS             <1..756
                     /locus_tag="PLF56_13070"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_002519499.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="transketolase C-terminal domain-containing
                     protein"
                     /protein_id="HOQ54528.1"
                     /translation="GYRTVVEIQFDGFVYPAFDQIISQVSRLHFRSHGRLTVPIVVRI
                     PYGGGIGAIEHHSESPEAYFAHTPGLKVVTCSNPLDAYWMIQQAIASDNPVIFFEPKR
                     RYHEKGEVDTEAEPMPLYQARVVKPGDDLTLISYGPMVRTCVEAAIAAGEEGRDIEVI
                     DLRSLAPLDMATVVESVRKTGRAIVVHEAALTLGMGAEIAARLQQDCFYVLQAPVLRV
                     TGYDIPYPPSRVESAFLPDLDRVLDAVDRSLTY"
     gene            800..2215
                     /locus_tag="PLF56_13075"
     CDS             800..2215
                     /locus_tag="PLF56_13075"
                     /EC_number="2.3.1.-"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_002514517.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_process: GO:0006096 - glycolytic process [Evidence
                     IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="dihydrolipoamide acetyltransferase family
                     protein"
                     /protein_id="HOQ54529.1"
                     /translation="MKEFRLPDPGEGLVEADIVRWVVSEGDEVKVNDVLVEIETAKSI
                     VELPSPWAGRIARLIADEGATVEVGAPIVLIDDGVAAPETPAETPDPVVEPAETPGGS
                     EAFLVGYGVKSSSLARRPRKSGAAIGKPIGPVTEHVQDAYAADQPVSRPIDQATTTGA
                     LKAEASGDPLPRPGQAPMSASASKRALAKPPVRRLARDLGLDLDAVDGTGPDGTITAD
                     DVIAAAVAARGGETRLSPRPSERVPLKGVRREMARTMAESFKVPTATVIMQADVTASM
                     ELVERLKNRREFAGLRVSPLLIFAKAACLAIARNPDLNSTLDMGASEIVRNENVNLGI
                     AAATPRGLMVPNIKAANRMNLLQLAEALHNMVQVAKTGKVPTEDLTKGTFTITNVGVF
                     GVDGGTPILNLGESAIMCLGTIARRPWVIGKGADEQIVIRSVCTLSLTFDHRLVDGEL
                     ASKFLSDVAAILGDPGLAMMF"
BASE COUNT          390 a          809 c          821 g          363 t
ORIGIN      
        1 ggctaccgca ccgtggtcga gattcagttc gacgggttcg tgtacccggc cttcgaccag
       61 atcatcagcc aggtctccag gctgcacttc cgttcgcacg gccggttgac ggtgccgatc
      121 gtggtgcgca ttccctatgg cggcggcatc ggcgcgatcg agcaccactc cgaatcgccc
      181 gaggcgtact tcgcgcacac gccggggctg aaggtggtga cctgctccaa tccactggac
      241 gcctactgga tgattcagca ggcgatcgcg tccgacaacc cggtgatctt cttcgagccg
      301 aagcgccgct accacgagaa gggcgaggtc gacaccgagg ccgagccgat gccgctgtac
      361 caggcccgtg tggtcaagcc cggcgacgac ctgaccctga tcagctacgg cccgatggtg
      421 cgcacctgcg tcgaggcggc gatcgcggcg ggcgaggagg gccgtgacat cgaagtgatc
      481 gacctgcgtt cgctggcgcc gctcgacatg gccacggtgg tcgaatccgt gcgcaagacc
      541 ggacgcgcga tcgtcgtcca cgaggcggcc ctcaccctcg gcatgggtgc cgagatcgcg
      601 gcccggttgc agcaggattg cttctacgtg ctgcaggctc cggtgctgcg cgtgaccggc
      661 tacgacattc cgtatccgcc cagtcgggtc gagagcgcgt tcctgcccga cctcgaccgg
      721 gtgctggacg ccgtcgaccg ttccttgacc tactgaccac cgcccaccca ccaagagctg
      781 ctgacaaggg gagattgcga tgaaggagtt ccggctaccc gatcccggtg aaggactggt
      841 cgaggccgac atcgtccggt gggtggtgag cgaaggtgac gaggtcaagg tcaacgacgt
      901 gctggtcgag atcgaaaccg cgaagtcgat cgtcgaactg ccttcgccct gggccggacg
      961 catcgcgcga ctgatcgcgg acgagggcgc caccgtcgag gtgggtgcgc cgatcgtgct
     1021 gatcgacgac ggcgtcgctg ccccggagac cccggccgag acccccgacc cggtggtcga
     1081 gccggccgag actcccggcg ggtccgaggc gttcctggtc ggctacggcg tcaagtcgtc
     1141 gtcgttggcg cgccgacccc gcaagtccgg cgcagccatc ggcaagccga tcggccccgt
     1201 caccgaacac gtgcaggacg cgtacgcggc ggaccagccg gtgtcgcgtc cgatcgacca
     1261 ggcgaccacc accggagcgt tgaaagccga agccagcggc gacccgctgc ctcggccggg
     1321 ccaggcgccg atgtcggcct cggcctcgaa acgggccctg gccaagccgc cggtccggcg
     1381 actggcccgc gacctcggtc tcgacctgga cgcggtcgac ggcaccggac ccgacggcac
     1441 catcaccgcc gacgacgtga tcgcggccgc ggtggcggcg cgcggtggtg agacgcggct
     1501 gagcccgcgg ccgtccgagc gggtgccgct caagggtgtg cgccgcgaga tggcccgcac
     1561 catggccgag tcgttcaagg tgccgaccgc aacggtgatc atgcaggccg acgtgacggc
     1621 gtcgatggag ttggtggagc ggctcaagaa ccgtcgcgag ttcgccgggc tgcgggtctc
     1681 accgctgctg atcttcgcca aggcggcctg cctggcgatc gcccgcaacc ccgatctgaa
     1741 ctcgaccctc gacatgggcg caagtgagat cgtccgcaac gagaacgtga acctcggcat
     1801 cgcggcggcc accccgcggg ggctgatggt gcccaacatc aaggccgcca accggatgaa
     1861 cctgctgcag ttggccgagg cgctgcacaa catggtgcag gtggccaaga ccggcaaggt
     1921 gccgaccgaa gacctgacca aaggcacctt cacgatcacc aacgtcgggg tgttcggggt
     1981 ggacggcggt acgccgatcc tcaatctggg cgagtcggcg atcatgtgcc tgggcacgat
     2041 cgcgcggcgt ccgtgggtga tcggcaaggg cgcggacgag cagatcgtga tccggtcggt
     2101 gtgcaccctg tcgctgacct tcgaccaccg gctggtggac ggcgagctgg cctcgaagtt
     2161 cctcagcgac gtcgcggcaa tattgggcga ccccggcctg gcgatgatgt tctgagaccc
     2221 tcccgtcatc ccggcgaacg ccggatagaa gttgcccctc attctggccg accccggcct
     2281 ggcgatgagg ttccgaggcc ctcccgtcat agtggcccgg agaccgcccc ccgttctgtc
     2341 acggctctgg gacccactgc tctgccgcgc gtgagaagct gag
//
LOCUS       DAOSUG010000486         2376 bp    DNA     linear   ENV 10-JUN-2024
DEFINITION  MAG TPA_asm: Micropruina sp. isolate AMR_MDS_2195
            NODE_18091_length_2376_cov_4.231366, whole genome shotgun sequence.
ACCESSION   DAOSUG010000486 DAOSUG010000000
VERSION     DAOSUG010000486.1
DBLINK      BioProject: PRJNA925477
            BioSample: SAMN32864262
            Sequence Read Archive: SRR4236673
KEYWORDS    WGS; ENV; Metagenome Assembled Genome; MAG; Third Party Data; TPA;
            TPA:assembly.
SOURCE      Micropruina sp. (wastewater metagenome)
  ORGANISM  Micropruina sp.
            Bacteria; Bacillati; Actinomycetota; Actinomycetes;
            Propionibacteriales; Nocardioidaceae; Micropruina.
REFERENCE   1  (bases 1 to 2376)
  AUTHORS   Abdulkadir,N., Saraiva,J.P., Zhang,J., Stolte,S., Gillor,O.,
            Harms,H. and Rocha,U.
  TITLE     Genome-centric analyses of 165 metagenomes show that mobile genetic
            elements are crucial for the transmission of antimicrobial
            resistance genes to pathogens in activated sludge and wastewater
  JOURNAL   Microbiol Spectr 12 (3), e0291823 (2024)
   PUBMED   38289113
REFERENCE   2  (bases 1 to 2376)
  AUTHORS   Abdulkadir,N.
  TITLE     Direct Submission
  JOURNAL   Submitted (24-MAR-2023) Environmental Microbiology, Helmholtz
            Center for Environmental Research -UFZ, Permoserstr 15, Leipzig
            04318, Germany
COMMENT     The annotation was added by the NCBI Prokaryotic Genome Annotation
            Pipeline (PGAP). Information about PGAP can be found here:
            https://www.ncbi.nlm.nih.gov/genome/annotation_prok/
            
            ##Genome-Assembly-Data-START##
            Assembly Method        :: SPAdes v. 3.11.1
            Genome Representation  :: Full
            Expected Final Version :: Yes
            Genome Coverage        :: Not Applicable
            Sequencing Technology  :: Illumina MiSeq
            ##Genome-Assembly-Data-END##
            
            ##Genome-Annotation-Data-START##
            Annotation Provider               :: NCBI
            Annotation Date                   :: 05/04/2023 04:57:10
            Annotation Pipeline               :: NCBI Prokaryotic Genome
                                                 Annotation Pipeline (PGAP)
            Annotation Method                 :: Best-placed reference protein
                                                 set; GeneMarkS-2+
            Annotation Software revision      :: 6.5
            Features Annotated                :: Gene; CDS; rRNA; tRNA; ncRNA
            Genes (total)                     :: 2,776
            CDSs (total)                      :: 2,750
            Genes (coding)                    :: 2,714
            CDSs (with protein)               :: 2,714
            Genes (RNA)                       :: 26
            tRNAs                             :: 24
            ncRNAs                            :: 2
            Pseudo Genes (total)              :: 36
            CDSs (without protein)            :: 36
            Pseudo Genes (ambiguous residues) :: 0 of 36
            Pseudo Genes (frameshifted)       :: 2 of 36
            Pseudo Genes (incomplete)         :: 32 of 36
            Pseudo Genes (internal stop)      :: 2 of 36
            ##Genome-Annotation-Data-END##
FEATURES             Location/Qualifiers
     source          1..2376
                     /organism="Micropruina sp."
                     /mol_type="genomic DNA"
                     /submitter_seqid="NODE_18091_length_2376_cov_4.231366"
                     /isolate="AMR_MDS_2195"
                     /isolation_source="Activated sludge of municipal
                     wastewater treatment plants"
                     /db_xref="taxon:2737536"
                     /environmental_sample
                     /geo_loc_name="Canada"
                     /lat_lon="49.25 N 123.1 W"
                     /collection_date="2015-06-16"
                     /metagenome_source="wastewater metagenome"
                     /note="metagenomic"
     gene            complement(13..237)
                     /locus_tag="PLF56_13080"
     CDS             complement(13..237)
                     /locus_tag="PLF56_13080"
                     /inference="COORDINATES: ab initio
                     prediction:GeneMarkS-2+"
                     /note="Derived by automated computational analysis using
                     gene prediction method: GeneMarkS-2+."
                     /codon_start=1
                     /transl_table=11
                     /product="hypothetical protein"
                     /protein_id="HOQ54530.1"
                     /translation="MTADQQVLVASLLLALAAYLHTAPFRLHAQVMHPRGAGPPSSTR
                     TALHAMLTPVEGAPSAVSRLAAAVIAGCVL"
     gene            complement(234..1034)
                     /locus_tag="PLF56_13085"
     CDS             complement(234..1034)
                     /locus_tag="PLF56_13085"
                     /inference="COORDINATES: protein motif:HMM:NF012694.2"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="type II secretion system F family protein"
                     /protein_id="HOQ54531.1"
                     /translation="MTILAGALLAVAVLTWQPRAPSARLRARAPARRAPLRMRLVPLL
                     VAAVVLSTLAGALLGAERGLALGLSASASSAIVTWTLAHARRARRQARSRDQVARGCI
                     ELAALLRAGHPPGRALQVVAESVPLFAEPAAHVRVGGDLAEALQRAGARPGCHGLRGV
                     AGAWRIAERTGASMTAFLDDLAAHLIAERDLRRSVDTELAAARMTGRLLAVLPLVGIG
                     LGYAVGGDPVAYLTTSTPGLMCLAVGTAAAVAGVVWSEKLADRAGELR"
     gene            complement(1031..2176)
                     /locus_tag="PLF56_13090"
     CDS             complement(1031..2176)
                     /locus_tag="PLF56_13090"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_013865819.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="TadA family conjugal transfer-associated ATPase"
                     /protein_id="HOQ54532.1"
                     /translation="MTQVQLDEVRAVLGSTGSVLSPSSVAGALRGLGLLVSEGTVRDT
                     VEALRRESAGAGPLEPLLALRGVTDVLVNGPQQVFVDRGAGLEQVDSPFEDESDVRRL
                     AVQLAAVVGRRLDEGCPFVDARMPNGTRVHAVLGVLTDVGTCLSLRVPARTSLTLDDW
                     VANRTLHARAADLLDRLVQVRAAFLISGGTGSGKTTLLATMLGLVEPSHRIVVVEDSR
                     ELAPAHPHVVRLEARPANAEGAGAVGLSALVRQALRMRPDRLVVGEVRGAELADLLTA
                     LNTGHEGGCGTVHANSAADVPARLEALAALGGMPRHALHAQAAAAIDLLVHIIRLPDG
                     RRWVHELRLLQWSETDALCRPVPAWTFPAAGDPVRHPGADRLAEVLR"
     gene            complement(2173..>2376)
                     /locus_tag="PLF56_13095"
     CDS             complement(2173..>2376)
                     /locus_tag="PLF56_13095"
                     /inference="COORDINATES: ab initio
                     prediction:GeneMarkS-2+"
                     /note="Derived by automated computational analysis using
                     gene prediction method: GeneMarkS-2+."
                     /codon_start=1
                     /transl_table=11
                     /product="hypothetical protein"
                     /protein_id="HOQ54533.1"
                     /translation="AVELVVRQQPGARVPSDVAEDVLGSPVVAHIRHEPAVAAAAERG
                     ERPPLHRRRGGLGRVCDALLAAT"
BASE COUNT          384 a          884 c          827 g          281 t
ORIGIN      
        1 cccgccggcc acctacaaga cgcaaccggc gatcacggcc gcggccagcc tgctcaccgc
       61 gctgggggcg ccctcgaccg gtgtgagcat ggcgtgcagc gcggtgcgtg tggacgaggg
      121 cgggccggca ccacggggat gcatcacctg cgcgtgcaac cggaacggcg cggtgtgaag
      181 gtaggccgcc agcgccagca acagactcgc caccagcacc tgttggtcgg cggtcatcga
      241 agctccccgg cccgatcggc gagcttttcg ctccacacca cccccgccac cgccgcagcg
      301 gtgccgacag ccagacacat caggcccggt gtggacgtcg tcaggtacgc caccgggtcg
      361 ccacccaccg cgtagccgag accgatgccg accagcggca ggacggccag gagccggccg
      421 gtcattcgag ctgcggcgag ttcggtgtcc acgctgcggc gcaggtcacg ctcggcgatc
      481 agatgggcag caaggtcgtc caagaacgcg gtcatggacg cgcccgtgcg ttcggcgatg
      541 cgccaggcgc cggcaactcc gcgtaggccg tgacagcccg gacgtgcccc cgcacgctgc
      601 aaggcctcgg ccaggtcgcc acccacccgc acgtgggcgg caggctcggc gaacagcgga
      661 accgattcag cgaccacctg cagcgcccgc cccggagggt gtccggcccg cagcagcgcg
      721 gccaattcaa tgcacccacg agccacctga tcgcgcgatc gcgcttgccg ccgggctcgc
      781 cgcgcgtgtg ccaacgtcca cgtcacgatc gcgctcgatg ccgaggcact caggccaagc
      841 gccagaccac gctcggcgcc cagcagggca ccggccagcg tcgagaggac gacggctgcg
      901 acgagcagcg gcaccaaccg catgcgcaac ggtgcgcgcc gcgcgggggc ccgggcacgg
      961 agccgggccg agggcgcacg tggctgccaa gtcaggacgg cgaccgccag cagtgcaccg
     1021 gcgaggatag tcatcgcagc acctcggcca accggtcggc acccgggtgc cgcaccggat
     1081 cgccggccgc cggaaaggtc catgccggta ccggacggca cagggcgtcg gtttcgctcc
     1141 actgcaacag gcggagttcg tgcacccagc gtcgcccgtc gggcagccgg atgatgtgca
     1201 ccaacagatc gatcgccgcg gctgcctgcg cgtgcagggc gtgccggggc atgccaccca
     1261 gcgcggccag tgcctcgagc cgggccggga cgtcggctgc ggagttggcg tgcactgtgc
     1321 cgcacccgcc ttcgtggccg gtgttgaggg ccgtgagtag atcggccagt tcagcacctc
     1381 gcacctcacc caccaccagc cggtcggggc gcatgcgcag tgcctggcgc accagtgcgg
     1441 acagccctac agcgcccgca ccctcggcat tggccgggcg ggcctcgagc cgtaccacgt
     1501 gggggtgcgc gggcgcgagt tctcgcgagt cttcgaccac cacgatgcga tggctgggct
     1561 cgaccagacc gagcatggtg gccagcagcg tggtcttgcc cgagccggtg ccacccgaga
     1621 tgagaaaggc cgcccgcacc tgcaccagcc gatcgagcag atcggcggcc cgcgcatgca
     1681 aggtgcggtt ggccacccag tcgtccaggg tcaggctggt gcgcgccggt actcgtagcg
     1741 aaaggcatgt gccgacatcg gtgagcacgc cgagcacggc atgcacccgg gtgccgttgg
     1801 gcatgcgggc gtccacgaag gggcagccct catcgagtcg cctgccgacg accgccgcga
     1861 gctgcacggc caatcgccgc acgtcgctct cgtcttcgaa cgggctgtcg acctgctcga
     1921 ggcccgcgcc ccgatcgacg aacacctgct gcggcccgtt gaccagcaca tcggtgacgc
     1981 cgcgaagtgc cagcagcggt tcgagtggac cggcacccgc gctctcacgg cgaagcgcct
     2041 cgacggtgtc gcgcacggta ccttcggaga cgagcaaccc gaggcctcgc agggcgccgg
     2101 ccaccgagct cggactgagc acggaacccg tactgccaag caccgcgcga acctcatcga
     2161 gctgtacctg ggtcatgtcg ccgccagcaa cgcatcgcac acccggccca ggcccccgcg
     2221 acgccggtgc aacggcggac gctcgccgcg ctcggcagcc gcagcgacgg cgggctcgtg
     2281 ccggatgtgc gccaccactg gagagcccag cacgtcttca gccacgtcgc tgggcactcg
     2341 ggcgccgggc tgctggcgca ccacgagttc gacggc
//
LOCUS       DAOSUG010000487         2375 bp    DNA     linear   ENV 10-JUN-2024
DEFINITION  MAG TPA_asm: Micropruina sp. isolate AMR_MDS_2195
            NODE_18103_length_2375_cov_4.377586, whole genome shotgun sequence.
ACCESSION   DAOSUG010000487 DAOSUG010000000
VERSION     DAOSUG010000487.1
DBLINK      BioProject: PRJNA925477
            BioSample: SAMN32864262
            Sequence Read Archive: SRR4236673
KEYWORDS    WGS; ENV; Metagenome Assembled Genome; MAG; Third Party Data; TPA;
            TPA:assembly.
SOURCE      Micropruina sp. (wastewater metagenome)
  ORGANISM  Micropruina sp.
            Bacteria; Bacillati; Actinomycetota; Actinomycetes;
            Propionibacteriales; Nocardioidaceae; Micropruina.
REFERENCE   1  (bases 1 to 2375)
  AUTHORS   Abdulkadir,N., Saraiva,J.P., Zhang,J., Stolte,S., Gillor,O.,
            Harms,H. and Rocha,U.
  TITLE     Genome-centric analyses of 165 metagenomes show that mobile genetic
            elements are crucial for the transmission of antimicrobial
            resistance genes to pathogens in activated sludge and wastewater
  JOURNAL   Microbiol Spectr 12 (3), e0291823 (2024)
   PUBMED   38289113
REFERENCE   2  (bases 1 to 2375)
  AUTHORS   Abdulkadir,N.
  TITLE     Direct Submission
  JOURNAL   Submitted (24-MAR-2023) Environmental Microbiology, Helmholtz
            Center for Environmental Research -UFZ, Permoserstr 15, Leipzig
            04318, Germany
COMMENT     The annotation was added by the NCBI Prokaryotic Genome Annotation
            Pipeline (PGAP). Information about PGAP can be found here:
            https://www.ncbi.nlm.nih.gov/genome/annotation_prok/
            
            ##Genome-Assembly-Data-START##
            Assembly Method        :: SPAdes v. 3.11.1
            Genome Representation  :: Full
            Expected Final Version :: Yes
            Genome Coverage        :: Not Applicable
            Sequencing Technology  :: Illumina MiSeq
            ##Genome-Assembly-Data-END##
            
            ##Genome-Annotation-Data-START##
            Annotation Provider               :: NCBI
            Annotation Date                   :: 05/04/2023 04:57:10
            Annotation Pipeline               :: NCBI Prokaryotic Genome
                                                 Annotation Pipeline (PGAP)
            Annotation Method                 :: Best-placed reference protein
                                                 set; GeneMarkS-2+
            Annotation Software revision      :: 6.5
            Features Annotated                :: Gene; CDS; rRNA; tRNA; ncRNA
            Genes (total)                     :: 2,776
            CDSs (total)                      :: 2,750
            Genes (coding)                    :: 2,714
            CDSs (with protein)               :: 2,714
            Genes (RNA)                       :: 26
            tRNAs                             :: 24
            ncRNAs                            :: 2
            Pseudo Genes (total)              :: 36
            CDSs (without protein)            :: 36
            Pseudo Genes (ambiguous residues) :: 0 of 36
            Pseudo Genes (frameshifted)       :: 2 of 36
            Pseudo Genes (incomplete)         :: 32 of 36
            Pseudo Genes (internal stop)      :: 2 of 36
            ##Genome-Annotation-Data-END##
FEATURES             Location/Qualifiers
     source          1..2375
                     /organism="Micropruina sp."
                     /mol_type="genomic DNA"
                     /submitter_seqid="NODE_18103_length_2375_cov_4.377586"
                     /isolate="AMR_MDS_2195"
                     /isolation_source="Activated sludge of municipal
                     wastewater treatment plants"
                     /db_xref="taxon:2737536"
                     /environmental_sample
                     /geo_loc_name="Canada"
                     /lat_lon="49.25 N 123.1 W"
                     /collection_date="2015-06-16"
                     /metagenome_source="wastewater metagenome"
                     /note="metagenomic"
     gene            complement(<1..1126)
                     /locus_tag="PLF56_13100"
     CDS             complement(<1..1126)
                     /locus_tag="PLF56_13100"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_013862572.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="hypothetical protein"
                     /protein_id="HOQ54534.1"
                     /translation="MTNEHHPVGLLLGAEEDWPRAFETVLGMVGPITVDGVTHTLSAE
                     RLTIEPFDLTAAVRQGLVIDRLAYWYYHPREWLKKAALVNGTYLLNSPFTFQSMEKHS
                     AYCAMLRLGLKVPRTVLVPYKNPVDNVRWAYTSAKYNQPFDLEAIAADLGYPLYMKPF
                     DGGGWRGVSRINNADDLRRAYDASGEMLMHLQATVDYDVFARALSIGPETMVMDFRPE
                     QPMHNRYHVSHGFLSARAGHETAAISRIVNAFFAWEFNSAEMLVAGDEVYPIDYANAC
                     PDVALTSLHYYFPWAIKALVRWSTYCLVTGRKAGVDLHTERYFAIADDPSLSYEAKID
                     EYLDLADQYFETDKYWAFCAEHLGHLDAAVLEWVRSGAFDR"
     gene            complement(1153..1899)
                     /locus_tag="PLF56_13105"
     CDS             complement(1153..1899)
                     /locus_tag="PLF56_13105"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_020387819.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="alpha/beta hydrolase-fold protein"
                     /protein_id="HOQ54535.1"
                     /translation="MDRYQLELDAPGFPHPGTVIRYGHWGRPVVVFPSEAGRAWDYEN
                     NGMVAAVQPLIDAGRIKLYCVDSFDRYTWSDAWLPLEERARRHGAYASWITNQVTGFI
                     GQDTPGAGDAVVTGCSLGAYHALQFALTRADLFPVAICLSGNYDPASWRAWGDRSEAA
                     YFANPTDYVPNLGGDHLDWLRSRLYAVLVAGQGAWETHPTGALPSTGRMAHLLGTKGL
                     HHELDLWGHDVSHDWHWWQRQIAHHLPRFC"
     gene            complement(1899..>2375)
                     /locus_tag="PLF56_13110"
     CDS             complement(1899..>2375)
                     /locus_tag="PLF56_13110"
                     /inference="COORDINATES: protein motif:HMM:NF012958.2"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="alpha/beta hydrolase-fold protein"
                     /protein_id="HOQ54536.1"
                     /translation="PLKAADAHAAGLVNEVLPTIGDRFRTAGKPVLMGQSLGALAALH
                     AARRYPGTFAGLFLQSGSFFTPALDPQESGFSHWAAVTGFVSTLYTTAAPERVPIMVV
                     CGTQEENLANNRALATHLAATHDEIGWGEVPDGHTWTTWRDLLDPHLTRLLTEAWR"
BASE COUNT          389 a          760 c          855 g          371 t
ORIGIN      
        1 ggcggtcgaa cgcacccgaa cgcacccact cgagcacggc cgcgtccaga tggccgagat
       61 gctcggcaca gaaggcccag tacttgtcgg tttcgaagta ctggtctgcc aggtcgaggt
      121 actcgtcgat cttggcctcg taactgagcg acgggtcgtc cgcgatcgcg aagtagcgtt
      181 cggtgtgcag gtcgacgccc gccttgcgtc cggtgaccag gcagtaggtg ctccagcgca
      241 ccagcgcctt gatcgcccac ggaaagtagt agtgcagcga ggtgagcgcc acgtcggggc
      301 aggcgttggc gtagtcgatc gggtacacct catcgccggc gacaagcatt tcggccgagt
      361 tgaactccca ggcgaagaac gcgttcacga tacggctgat cgcggcggtc tcgtgaccgg
      421 cccgggccga gagaaagccg tgcgagacgt ggtagcggtt gtgcatcggc tgctcgggac
      481 gaaagtccat caccatcgtt tcgggcccga tcgagagcgc ccgggcgaac acgtcgtagt
      541 cgacggtggc ctgcaggtgc atcagcatct cgccggaggc gtcgtaggcc cggcgcaggt
      601 cgtcggcgtt gttgatccgc gacacgccac gccagccgcc gccgtcgaac ggcttcatgt
      661 acagcgggta accgaggtcg gcggcgatcg cctcgaggtc gaagggctgg ttgtacttgg
      721 ccgaggtgta ggcccagcgc acgttgtcga ccgggttctt gtagggcacg agcaccgtgc
      781 ggggcacctt gaggccgagc cgcagcatgg cgcagtaggc gctgtgcttc tccatcgatt
      841 gaaacgtgaa ggggctgttg agcaggtagg tgccgttgac cagggcggcc ttcttcagcc
      901 actcgcgcgg gtggtagtac cagtacgcga gccggtcgat cacgagcccc tgtcgcacgg
      961 cggcggtcag gtcgaacggc tcgatggtga gccgctcggc cgacagagtg tgggtgacgc
     1021 cgtcgacggt gatcggcccc accatgccga gaacggtctc gaaggcgcgc ggccagtctt
     1081 cttcggcacc cagcagcagg cccaccggat ggtgctcgtt ggtcatgcgc agaccctagc
     1141 ggggagccgg tctcagcaga agcggggcag gtgatgtgcg atctgccgct gccaccagtg
     1201 ccagtcgtgt gacacgtcat gaccccacag gtcgagctcg tgatgcagcc ccttggtgcc
     1261 gagcaggtgc gccatgcgac ccgtggacgg cagcgccccc gtcgggtggg tctcccacgc
     1321 cccctggccg gcgaccagca ccgcgtacag ccgcgaccgc agccaatcga ggtggtcgcc
     1381 gcccaggttc ggcacatagt cggtcggatt ggcgaagtac gccgcctcgg aacgatcgcc
     1441 ccaggcccgc cacgacgccg ggtcgtagtt gccggacagg cagatggcca ccggaaacag
     1501 gtcggccctg gtgagcgcga actgcagggc atgataggcg ccgagcgagc aaccggtcac
     1561 cacggcatcg cccgcgccgg gggtgtcctg gccgatgaag ccggtgacct gattggtgat
     1621 ccaactcgcg taggcgccgt gccggcgggc gcgctcctcg agcggcagcc aggcgtccga
     1681 ccaggtgtag cggtcgaagc tgtcgacgca gtacagcttg atccggccgg catcgatcag
     1741 tggctggacg gccgccacca tgccgttgtt ctcgtaatcc caggcgcgtc cggcctcgga
     1801 cggaaagacc accaccggac ggccccagtg cccgtagcgg atcaccgtgc cggggtgcgg
     1861 aaagccgggc gcgtcgagtt cgagttgata gcggtccatc agcgccacgc ctccgtgagc
     1921 agtcgggtca ggtgggggtc gagcaggtcg cgccaggtcg tccaggtgtg gccgtcgggc
     1981 acctcgcccc agccgatctc gtcgtgggtc gcggccaggt gcgtggccag ggcccggttg
     2041 ttggcgaggt tctcctcctg ggttccgcag accaccatga tcggcacccg ttcgggcgcg
     2101 gcggtggtgt agagggtcga gacgaagccg gtcaccgccg cccagtgcga gaagccgctc
     2161 tcctgcggat cgagagcggg ggtgaagaac gagcccgact gcagaaacag gccggcgaag
     2221 gtgcccgggt agcgccgggc ggcgtgcagc gcggcaagag cgcccagtga ttgccccatc
     2281 agcaccggct tgccggcggt gcggaaccga tcgccgatcg tcggcagcac ctcgttgacc
     2341 agtccggcag cgtgcgcatc ggccgccttg agcgg
//
LOCUS       DAOSUG010000488         2371 bp    DNA     linear   ENV 10-JUN-2024
DEFINITION  MAG TPA_asm: Micropruina sp. isolate AMR_MDS_2195
            NODE_18153_length_2371_cov_3.930052, whole genome shotgun sequence.
ACCESSION   DAOSUG010000488 DAOSUG010000000
VERSION     DAOSUG010000488.1
DBLINK      BioProject: PRJNA925477
            BioSample: SAMN32864262
            Sequence Read Archive: SRR4236673
KEYWORDS    WGS; ENV; Metagenome Assembled Genome; MAG; Third Party Data; TPA;
            TPA:assembly.
SOURCE      Micropruina sp. (wastewater metagenome)
  ORGANISM  Micropruina sp.
            Bacteria; Bacillati; Actinomycetota; Actinomycetes;
            Propionibacteriales; Nocardioidaceae; Micropruina.
REFERENCE   1  (bases 1 to 2371)
  AUTHORS   Abdulkadir,N., Saraiva,J.P., Zhang,J., Stolte,S., Gillor,O.,
            Harms,H. and Rocha,U.
  TITLE     Genome-centric analyses of 165 metagenomes show that mobile genetic
            elements are crucial for the transmission of antimicrobial
            resistance genes to pathogens in activated sludge and wastewater
  JOURNAL   Microbiol Spectr 12 (3), e0291823 (2024)
   PUBMED   38289113
REFERENCE   2  (bases 1 to 2371)
  AUTHORS   Abdulkadir,N.
  TITLE     Direct Submission
  JOURNAL   Submitted (24-MAR-2023) Environmental Microbiology, Helmholtz
            Center for Environmental Research -UFZ, Permoserstr 15, Leipzig
            04318, Germany
COMMENT     The annotation was added by the NCBI Prokaryotic Genome Annotation
            Pipeline (PGAP). Information about PGAP can be found here:
            https://www.ncbi.nlm.nih.gov/genome/annotation_prok/
            
            ##Genome-Assembly-Data-START##
            Assembly Method        :: SPAdes v. 3.11.1
            Genome Representation  :: Full
            Expected Final Version :: Yes
            Genome Coverage        :: Not Applicable
            Sequencing Technology  :: Illumina MiSeq
            ##Genome-Assembly-Data-END##
            
            ##Genome-Annotation-Data-START##
            Annotation Provider               :: NCBI
            Annotation Date                   :: 05/04/2023 04:57:10
            Annotation Pipeline               :: NCBI Prokaryotic Genome
                                                 Annotation Pipeline (PGAP)
            Annotation Method                 :: Best-placed reference protein
                                                 set; GeneMarkS-2+
            Annotation Software revision      :: 6.5
            Features Annotated                :: Gene; CDS; rRNA; tRNA; ncRNA
            Genes (total)                     :: 2,776
            CDSs (total)                      :: 2,750
            Genes (coding)                    :: 2,714
            CDSs (with protein)               :: 2,714
            Genes (RNA)                       :: 26
            tRNAs                             :: 24
            ncRNAs                            :: 2
            Pseudo Genes (total)              :: 36
            CDSs (without protein)            :: 36
            Pseudo Genes (ambiguous residues) :: 0 of 36
            Pseudo Genes (frameshifted)       :: 2 of 36
            Pseudo Genes (incomplete)         :: 32 of 36
            Pseudo Genes (internal stop)      :: 2 of 36
            ##Genome-Annotation-Data-END##
FEATURES             Location/Qualifiers
     source          1..2371
                     /organism="Micropruina sp."
                     /mol_type="genomic DNA"
                     /submitter_seqid="NODE_18153_length_2371_cov_3.930052"
                     /isolate="AMR_MDS_2195"
                     /isolation_source="Activated sludge of municipal
                     wastewater treatment plants"
                     /db_xref="taxon:2737536"
                     /environmental_sample
                     /geo_loc_name="Canada"
                     /lat_lon="49.25 N 123.1 W"
                     /collection_date="2015-06-16"
                     /metagenome_source="wastewater metagenome"
                     /note="metagenomic"
     gene            complement(<1..1810)
                     /locus_tag="PLF56_13115"
     CDS             complement(<1..1810)
                     /locus_tag="PLF56_13115"
                     /inference="COORDINATES: protein motif:HMM:NF012770.2"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_function: GO:0016787 - hydrolase activity [Evidence
                     IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="alpha/beta hydrolase"
                     /protein_id="HOQ54537.1"
                     /translation="MRTLVRFLGVGAAIAVAASCVTAVGSPRAAAGTPAIVPARTAPA
                     AKARVVSAKTSAIEKARVDAVKTTTLSWYSCYTDFKCATVTLPLDYDQPKGPQVEVAV
                     LKRPANDRRHRIGSTFVNPGGPGGSGIELAQNADANFSGALLDRFDIVGFDPRGVGFS
                     SNVRCFANNAQNESVLGVLGSTPFPDTAAETRAFIKATNTHAAACSGTGLPLTAAVST
                     AEVARDMDVLRRAVGDRKLNYLGFSYGSYLGQVYANLFPDRVRSLAIDGVINPTAWAG
                     TAATAATPLGDRLRAADGASKALHEILVRCDRAGGAACSFAPGDPVANWRLIADRLKA
                     KPLDLDGTYGYPELAGDALTALYYDAGADMIADLGSSLIVLTEPPAVRPKSMNNATSA
                     VAATRAAAARKAALSSLRAVHRRLAALRVPATLPGAATARARLGFPYDNDHDALQTIA
                     CTDSLNAPVLASLPDLAAAADRRAPYFGALWAWNNASCASAKWTATDEDAYRGPFNRR
                     TVKPVLVVGNYWDPATNYAGAVAASRLLPNSRLLSSDSWGHTAYGSSDCVTAAVDRYL
                     LKGTLPKKGKVCVGDSQPFDGSGTEIDSLQRRVLGPL"
     gene            1996..>2371
                     /locus_tag="PLF56_13120"
     CDS             1996..>2371
                     /locus_tag="PLF56_13120"
                     /EC_number="2.1.2.1"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_017935211.1"
                     /note="catalyzes the reaction of glycine with
                     5,10-methylenetetrahydrofolate to form L-serine and
                     tetrahydrofolate; Derived by automated computational
                     analysis using gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="glycine hydroxymethyltransferase"
                     /protein_id="HOQ54538.1"
                     /translation="MTDLATTASSIYSQILDVIGGVEPRIADATRAELADQRASLKLI
                     ASENYASLPVLMTMGTWLSDKYAEGTIGHRFYAGCQNVDTVESVAAEHARELFGAEYA
                     YLQPHSGIDANLVAYWSILAHRV"
BASE COUNT          366 a          794 c          851 g          360 t
ORIGIN      
        1 gcagtggacc caggacgcgc cgctgcagcg aatcgatctc ggtgccgctg ccgtcgaacg
       61 gctggctgtc gccgacgcac accttgccct tcttgggcag ggtgcccttg agcaggtacc
      121 ggtcgaccgc cgcagtgaca cagtcggagc tgccgtaggc ggtgtgcccc cacgagtcgg
      181 acgacagcag ccggctgttc ggcaacagtc tcgacgccgc caccgcgccc gcatagttcg
      241 tggccgggtc ccagtagttt ccgacgacca gcaccggctt gaccgtgcgg cggttgaagg
      301 ggccgcggta ggcatcttcg tcggtcgcgg tccacttggc gctggcgcaa ctggcgttgt
      361 tccaggccca cagcgcaccg aagtaggggg cccggcggtc ggcggccgcg gccaggtcgg
      421 gcaggctggc caggaccgga gcgttcagcg agtcggtgca cgcgatggtc tgtaaggcgt
      481 cgtggtcgtt gtcatacgga aagccgagcc gggcacgtgc agtggccgcg cccggcaacg
      541 tggccggcac ccgcagcgct gccagccgcc tgtgcacagc gcgcaacgag ctcagggccg
      601 ccttgcgggc agccgccgcg cgcgtcgccg ccacagccga ggtggcgttg ttcatgctct
      661 tcgggcggac ggccggtggt tcggtgagca cgatcagcga gctgcccagg tcggcgatca
      721 tgtcggcacc ggcgtcgtag tagagggcgg tgagcgcatc gccggccagt tcggggtagc
      781 cgtaggtgcc gtccaggtcg agcggtttgg ccttcaaccg gtcggcgatc aggcgccagt
      841 tggccaccgg atcgccgggt gcgaagctgc aggccgcgcc gcccgcgcgg tcacagcgca
      901 ccagaatctc gtgcagggcc ttggacgctc cgtcggctgc gcggagccgg tcacccagcg
      961 gcgtggcggc ggtcgcggcg gtaccggccc aggcggtggg gttgatcacc ccgtcgatgg
     1021 cgagcgaacg aacgcgatcc ggaaaaaggt tcgcgtacac ctgacccagg tacgagccgt
     1081 acgagaaacc cagatagttc agcttccggt cgccgaccgc gcggcgcagc acgtccatgt
     1141 cgcgggccac ctcggcggtc gacaccgcag cggtgagcgg caggccggtg cccgaacagg
     1201 ccgcggcgtg ggtgttcgtg gccttgatga acgccctggt ctcggccgcg gtgtcgggga
     1261 acggtgtgct gcccaacacg ccgagcaccg actcgttctg cgcattgttg gcgaagcacc
     1321 gcacgttgct gctgaacccg acgccgcgcg ggtcgaagcc gacgatgtcg aagcggtcga
     1381 gcagggcgcc cgagaagttg gcgtcggcgt tctgggccaa ctcgatgccc gagccgcccg
     1441 gaccgcccgg attcacgaac gtgctgccga tgcggtgccg gcgatcgttc gccggccgct
     1501 tgagcacggc gacttcgacc tgcgggccct tgggctggtc gtagtcgagc ggcagcgtga
     1561 ccgtggcgca cttgaagtcg gtgtagcacg agtaccacga caacgtggtc gtcttgacgg
     1621 cgtccacgcg cgccttctcg atcgctgagg tcttggcgct gaccaccctg gccttcgcgg
     1681 ccggcgccgt tcgagcaggg acgatggccg gtgtgccggc cgcggcacgc gggctgccga
     1741 cggcggtcac acacgaggcg gcgacagcga tcgccgcacc cactccgaga aagcggacga
     1801 gtgtacgcac ggagtgctcc tgacggtgaa gtgcacgtcc cggtggtccg ggcgcattga
     1861 acctagcgga cgcgtctgac attcggcagt gggaccatgc gcgacgagtc gtcggcgaga
     1921 ccgcccccgg gcgtgacgcg gctcgcggac ggatctcgat agagttggtg gggtcgctcg
     1981 acaacaggag aacccgtgac cgatctcgcc accaccgcct cgtccatcta cagccagatt
     2041 ctcgacgtga tcggcggtgt cgagccgcgg atcgccgatg ccacccgggc cgaactggcc
     2101 gaccagcggg cctcgctcaa gctgatcgcc agcgagaact acgcctcgct gccggtactg
     2161 atgaccatgg gtacctggtt gtcggacaaa tacgccgagg gcaccatcgg gcaccgcttc
     2221 tacgcgggct gccagaacgt cgacacggtc gaatcggtgg ccgccgagca cgcccgcgaa
     2281 ctgttcggcg cagaatacgc ctacctgcag ccgcattcgg gcatcgacgc caacctggtc
     2341 gcctactggt cgatcctggc ccaccgggtg g
//
LOCUS       DAOSUG010000489         2364 bp    DNA     linear   ENV 10-JUN-2024
DEFINITION  MAG TPA_asm: Micropruina sp. isolate AMR_MDS_2195
            NODE_18219_length_2364_cov_4.010827, whole genome shotgun sequence.
ACCESSION   DAOSUG010000489 DAOSUG010000000
VERSION     DAOSUG010000489.1
DBLINK      BioProject: PRJNA925477
            BioSample: SAMN32864262
            Sequence Read Archive: SRR4236673
KEYWORDS    WGS; ENV; Metagenome Assembled Genome; MAG; Third Party Data; TPA;
            TPA:assembly.
SOURCE      Micropruina sp. (wastewater metagenome)
  ORGANISM  Micropruina sp.
            Bacteria; Bacillati; Actinomycetota; Actinomycetes;
            Propionibacteriales; Nocardioidaceae; Micropruina.
REFERENCE   1  (bases 1 to 2364)
  AUTHORS   Abdulkadir,N., Saraiva,J.P., Zhang,J., Stolte,S., Gillor,O.,
            Harms,H. and Rocha,U.
  TITLE     Genome-centric analyses of 165 metagenomes show that mobile genetic
            elements are crucial for the transmission of antimicrobial
            resistance genes to pathogens in activated sludge and wastewater
  JOURNAL   Microbiol Spectr 12 (3), e0291823 (2024)
   PUBMED   38289113
REFERENCE   2  (bases 1 to 2364)
  AUTHORS   Abdulkadir,N.
  TITLE     Direct Submission
  JOURNAL   Submitted (24-MAR-2023) Environmental Microbiology, Helmholtz
            Center for Environmental Research -UFZ, Permoserstr 15, Leipzig
            04318, Germany
COMMENT     The annotation was added by the NCBI Prokaryotic Genome Annotation
            Pipeline (PGAP). Information about PGAP can be found here:
            https://www.ncbi.nlm.nih.gov/genome/annotation_prok/
            
            ##Genome-Assembly-Data-START##
            Assembly Method        :: SPAdes v. 3.11.1
            Genome Representation  :: Full
            Expected Final Version :: Yes
            Genome Coverage        :: Not Applicable
            Sequencing Technology  :: Illumina MiSeq
            ##Genome-Assembly-Data-END##
            
            ##Genome-Annotation-Data-START##
            Annotation Provider               :: NCBI
            Annotation Date                   :: 05/04/2023 04:57:10
            Annotation Pipeline               :: NCBI Prokaryotic Genome
                                                 Annotation Pipeline (PGAP)
            Annotation Method                 :: Best-placed reference protein
                                                 set; GeneMarkS-2+
            Annotation Software revision      :: 6.5
            Features Annotated                :: Gene; CDS; rRNA; tRNA; ncRNA
            Genes (total)                     :: 2,776
            CDSs (total)                      :: 2,750
            Genes (coding)                    :: 2,714
            CDSs (with protein)               :: 2,714
            Genes (RNA)                       :: 26
            tRNAs                             :: 24
            ncRNAs                            :: 2
            Pseudo Genes (total)              :: 36
            CDSs (without protein)            :: 36
            Pseudo Genes (ambiguous residues) :: 0 of 36
            Pseudo Genes (frameshifted)       :: 2 of 36
            Pseudo Genes (incomplete)         :: 32 of 36
            Pseudo Genes (internal stop)      :: 2 of 36
            ##Genome-Annotation-Data-END##
FEATURES             Location/Qualifiers
     source          1..2364
                     /organism="Micropruina sp."
                     /mol_type="genomic DNA"
                     /submitter_seqid="NODE_18219_length_2364_cov_4.010827"
                     /isolate="AMR_MDS_2195"
                     /isolation_source="Activated sludge of municipal
                     wastewater treatment plants"
                     /db_xref="taxon:2737536"
                     /environmental_sample
                     /geo_loc_name="Canada"
                     /lat_lon="49.25 N 123.1 W"
                     /collection_date="2015-06-16"
                     /metagenome_source="wastewater metagenome"
                     /note="metagenomic"
     gene            complement(<1..242)
                     /locus_tag="PLF56_13125"
     CDS             complement(<1..242)
                     /locus_tag="PLF56_13125"
                     /EC_number="1.8.4.11"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_013865292.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_function: GO:0008113 - peptide-methionine (S)-S-oxide
                     reductase activity [Evidence IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="peptide-methionine (S)-S-oxide reductase"
                     /protein_id="HOQ54539.1"
                     /translation="MLFTKPTMVDPRVALRGRETPVLDQPFFHRVFGHRIDRVPEGAE
                     VAVLGLGCFWGAEKLFWNTPGVLNTAAGYAGGYTPNP"
     gene            complement(589..2085)
                     /gene="dacB"
                     /locus_tag="PLF56_13130"
     CDS             complement(589..2085)
                     /gene="dacB"
                     /locus_tag="PLF56_13130"
                     /EC_number="3.4.16.4"
                     /inference="COORDINATES: protein motif:HMM:NF014200.2"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_function: GO:0004175 - endopeptidase activity [Evidence
                     IEA];
                     GO_function: GO:0009002 - serine-type D-Ala-D-Ala
                     carboxypeptidase activity [Evidence IEA];
                     GO_process: GO:0009252 - peptidoglycan biosynthetic
                     process [Evidence IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="D-alanyl-D-alanine
                     carboxypeptidase/D-alanyl-D-alanine-endopeptidase"
                     /protein_id="HOQ54540.1"
                     /translation="MNLPSRLLLAPLLTLTLIVPTLAPTTAQAGPLTLPALASDAALA
                     AKLKSVLNNSRVTKATVGVVVADSASGTQLYGRSATTALKPASNMKLVTAAAALDLLG
                     PSSTFRTAVYAPSTPVNGAVDTLFLRGYGDPTLRESDLTSLAAQVRAAGVTTVTGRVI
                     GDGTFFDGDRYNNYWDPIDYNNAYAAQISGLTLSPSSAYAVGTVQVTYKPATGKGKKA
                     TLGVVPAAAAGYVKLVNKTTTTRVGTGASISVRRTNGTNRITVSGRVALKRSLVKRTV
                     TISDPARYAAHVFTRALRAAGVSVAASAGTGSTPKSRVQLASDSSAPLSSILKLLMKP
                     SNNGMTEHLLKTLGRVGTKAGTWKAGAAAVRTWLATTQSVPATVVLVDGSGLARRNKL
                     TARVLVRLLQYAQTRTWFDIFYDALPVAGNADPAIGGTLATRMVGTPAQNNLRAKTGT
                     LRGVTALSGYVTDANGRRYTFSMLGTYPTYSPRIVFDKVGATLAGWRS"
BASE COUNT          358 a          756 c          844 g          406 t
ORIGIN      
        1 gggttcggtg tgtagccgcc cgcgtagccc gctgcggtgt tgagtacgcc gggcgtgttc
       61 cagaacagct tctcagcccc ccagaagcaa cccaggccga gcacggccac ctcggcgccc
      121 tcgggcaccc ggtcgatccg gtgcccgaag acccggtgga agaacggttg gtcgagcacc
      181 ggcgtctcgc ggccgcgcag cgcgacccgc gggtcgacca tggtcggttt ggtgaacagc
      241 atgtttgcct cctgcatggt caaccgatcg tacgcgcggc gcattcctgg acgcgtgtgt
      301 tcacgggggt tgctcggcgg ccacggcatc gattctcacc gtgatccact cgatgtgtca
      361 ggggctcctc aggggttgca acaaggtgaa ggatcgtgat ccgacatcgg atcgcaatcc
      421 ttcacggtgt gcgccgccgg agtgcccggg ctgctcgccc cgtcgacggg cagcgcgcag
      481 caccacgaat gcccggcgag gccggggtga tcgccaccca ctgttgtgtc gctgcgacca
      541 agcatcgcta cgagcgtgct gaacacatcc cgttccgaga gaggacgttc agctgcgcca
      601 gccggccagg gtggcgccca ccttgtcgaa cacgatccgc ggcgagtacg tcgggtaggt
      661 gcccagcatc gaaaaggtgt agcgccgtcc gttggcatcg gtgacgtagc cgctcagcgc
      721 agtgacgccg cgcagcgtgc cggtcttggc gcgcaggttg ttctgcgccg gcgtgccgac
      781 catacgggtg gcgagcgtgc cgccgatcgc cgggtcggca ttgccggcca ccgggagggc
      841 gtcgtagaag atgtcgaacc acgtgcgcgt ctgcgcgtac tgaagtagcc gcaccagcac
      901 ccgtgcggtc agtttgttgc ggcgggcgag gcccgacccg tccaccagca cgacggtggc
      961 cggtaccgac tgtgtggtcg ccagccaggt gcggacggcg gccgcgccgg ccttccaggt
     1021 acccgccttg gtgcccacgc gacccagcgt cttgagcagg tgctcggtca tgccgttgtt
     1081 ggacggcttc atcagcaact tgaggatgct gctcagcggc gcggacgaat ccgaggccaa
     1141 ctgcacccgg ctcttcggcg tactgcccgt gcccgccgac gccgcgacgc ttacgcccgc
     1201 ggcgcgcagc gcgcgggtga acacatgcgc ggcataccgg gccggatcgc tgatggtgac
     1261 agtgcgcttg accaggctgc gcttgagcgc gacccgtccg gagacggtga tcctgttcgt
     1321 gccgttggtg cggcgcacac tgatggatgc acccgttccg acgcgcgtgg tcgtggtctt
     1381 gttgaccagc ttcacatacc ccgcggctgc agccggcacc acgccgagag tcgccttctt
     1441 gcctttgccg gtggcgggct tgtaggtgac ctgcacggtg ccgaccgcat acgccgacga
     1501 cggcgacagg gtcaggcccg aaatttgcgc ggcgtaggcg ttgttgtagt cgatcggatc
     1561 ccagtagttg ttgtaacgat cgccgtcgaa gaaggtgccg tcgccgatca cgcgtccggt
     1621 gaccgtggtg acacccgccg cgcgtacctg cgctgccaag gaagtcagat ccgattcgcg
     1681 cagggtgggg tcgccgtagc cccgcaagaa caaggtgtcg accgccccgt tgaccggtgt
     1741 gctgggggcg tagacggcgg tgcggaacgt gctgctcggg cccaacagat ccagcgcggc
     1801 agcggcggtg accagcttca tgttcgaggc cggcttcagg gctgtggtcg cggagcgtcc
     1861 gtagagctga gtgcccgagg cggagtcggc gaccaccacc cccacggttg ccttcgtcac
     1921 ccgcgagttg ttcagcaccg acttcaactt cgccgcgagc gccgcatcac tcgccagggc
     1981 agggagcgtg agggggccgg cctgtgccgt tgtcggggcg agcgtgggca cgatcagggt
     2041 gagggtgagc aggggagcga gcagcagccg tgagggcagg ttcatcggag actccaacgg
     2101 gggggacgtg tgccgtcacc ctgacacagt ggcgagcgag gatccagagc ggttgagaca
     2161 tttgtctcaa ccaactctca gggtgcgggg gtgatccgga ccggtctgcg tcgctctgtg
     2221 gtgatgatcg acgtgctgtg gctcgagtca tgtgtcggcc tggttggccg gtgccgccgc
     2281 tcctcgacgg cgcacggatg ccctcgtcgg tcggtcggac ggtccgggcc tgtgggtcct
     2341 atcgccgagg atgacaacgc agcg
//
LOCUS       DAOSUG010000490         2350 bp    DNA     linear   ENV 10-JUN-2024
DEFINITION  MAG TPA_asm: Micropruina sp. isolate AMR_MDS_2195
            NODE_18380_length_2350_cov_4.901089, whole genome shotgun sequence.
ACCESSION   DAOSUG010000490 DAOSUG010000000
VERSION     DAOSUG010000490.1
DBLINK      BioProject: PRJNA925477
            BioSample: SAMN32864262
            Sequence Read Archive: SRR4236673
KEYWORDS    WGS; ENV; Metagenome Assembled Genome; MAG; Third Party Data; TPA;
            TPA:assembly.
SOURCE      Micropruina sp. (wastewater metagenome)
  ORGANISM  Micropruina sp.
            Bacteria; Bacillati; Actinomycetota; Actinomycetes;
            Propionibacteriales; Nocardioidaceae; Micropruina.
REFERENCE   1  (bases 1 to 2350)
  AUTHORS   Abdulkadir,N., Saraiva,J.P., Zhang,J., Stolte,S., Gillor,O.,
            Harms,H. and Rocha,U.
  TITLE     Genome-centric analyses of 165 metagenomes show that mobile genetic
            elements are crucial for the transmission of antimicrobial
            resistance genes to pathogens in activated sludge and wastewater
  JOURNAL   Microbiol Spectr 12 (3), e0291823 (2024)
   PUBMED   38289113
REFERENCE   2  (bases 1 to 2350)
  AUTHORS   Abdulkadir,N.
  TITLE     Direct Submission
  JOURNAL   Submitted (24-MAR-2023) Environmental Microbiology, Helmholtz
            Center for Environmental Research -UFZ, Permoserstr 15, Leipzig
            04318, Germany
COMMENT     The annotation was added by the NCBI Prokaryotic Genome Annotation
            Pipeline (PGAP). Information about PGAP can be found here:
            https://www.ncbi.nlm.nih.gov/genome/annotation_prok/
            
            ##Genome-Assembly-Data-START##
            Assembly Method        :: SPAdes v. 3.11.1
            Genome Representation  :: Full
            Expected Final Version :: Yes
            Genome Coverage        :: Not Applicable
            Sequencing Technology  :: Illumina MiSeq
            ##Genome-Assembly-Data-END##
            
            ##Genome-Annotation-Data-START##
            Annotation Provider               :: NCBI
            Annotation Date                   :: 05/04/2023 04:57:10
            Annotation Pipeline               :: NCBI Prokaryotic Genome
                                                 Annotation Pipeline (PGAP)
            Annotation Method                 :: Best-placed reference protein
                                                 set; GeneMarkS-2+
            Annotation Software revision      :: 6.5
            Features Annotated                :: Gene; CDS; rRNA; tRNA; ncRNA
            Genes (total)                     :: 2,776
            CDSs (total)                      :: 2,750
            Genes (coding)                    :: 2,714
            CDSs (with protein)               :: 2,714
            Genes (RNA)                       :: 26
            tRNAs                             :: 24
            ncRNAs                            :: 2
            Pseudo Genes (total)              :: 36
            CDSs (without protein)            :: 36
            Pseudo Genes (ambiguous residues) :: 0 of 36
            Pseudo Genes (frameshifted)       :: 2 of 36
            Pseudo Genes (incomplete)         :: 32 of 36
            Pseudo Genes (internal stop)      :: 2 of 36
            ##Genome-Annotation-Data-END##
FEATURES             Location/Qualifiers
     source          1..2350
                     /organism="Micropruina sp."
                     /mol_type="genomic DNA"
                     /submitter_seqid="NODE_18380_length_2350_cov_4.901089"
                     /isolate="AMR_MDS_2195"
                     /isolation_source="Activated sludge of municipal
                     wastewater treatment plants"
                     /db_xref="taxon:2737536"
                     /environmental_sample
                     /geo_loc_name="Canada"
                     /lat_lon="49.25 N 123.1 W"
                     /collection_date="2015-06-16"
                     /metagenome_source="wastewater metagenome"
                     /note="metagenomic"
     gene            <1..245
                     /locus_tag="PLF56_13135"
     CDS             <1..245
                     /locus_tag="PLF56_13135"
                     /inference="COORDINATES: protein motif:HMM:NF012669.2"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=3
                     /transl_table=11
                     /product="DeoR/GlpR transcriptional regulator"
                     /protein_id="HOQ54541.1"
                     /translation="DLQCFHPYEDCVQVKRAMMTSAAQSILLLDHSKLNRRALHTFAA
                     MTDFDQVVVDSLTPESLVSDLRERGVSVVRAEAPAS"
     gene            378..869
                     /locus_tag="PLF56_13140"
     CDS             378..869
                     /locus_tag="PLF56_13140"
                     /EC_number="5.3.1.6"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_014846856.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_function: GO:0016853 - isomerase activity [Evidence
                     IEA];
                     GO_process: GO:0005975 - carbohydrate metabolic process
                     [Evidence IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="ribose-5-phosphate isomerase"
                     /protein_id="HOQ54542.1"
                     /translation="MGLRVVVAADTAGYDYKEQLKKDLEADNRVDEVIDVGVGSGEDV
                     DYPHVAVAGARLIADGKADRGLFVCGTGMGVAIAANKVPGIRASVAHDSFSVERLVLS
                     NNAQVLTFGQRVIGLELARRLAKEWLGYTFDPSSSSAPKVAALESYDKGDNGNEAVQT
                     SCD"
     gene            914..1924
                     /locus_tag="PLF56_13145"
     CDS             914..1924
                     /locus_tag="PLF56_13145"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_002512670.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="hypothetical protein"
                     /protein_id="HOQ54543.1"
                     /translation="MFTSDIAIFLLGLIAAIFGGYFGAAIGGNFAFALTGFMILVSWG
                     IFVGSGSDIGFNYLAFGPVMGPHITFAAGCAAAAYAMHKGLIESGRDATSPLARLGRV
                     DVLLVGAAFGAFGYLFNIGLSFIPWFGSHIDSVALTVFTSNVLARLIWGNSLMVTQKY
                     NKGATNFFGKIAPNETDFWLRYQEKPSQYLPLGIFAGGLAAGAGIMLGHFVPGGAAFA
                     HTLPFAISAIIILFLILGAEMPVQHHITIIGALAAVKFMPVLAGAGFEWNGVWTSATW
                     MTAIGAILIGCVFAMISAFLAEFQSQLFHQRGNTHIDPPAAAIWSTTLLVLAISGALT
                     HA"
     gene            1954..>2350
                     /locus_tag="PLF56_13150"
     CDS             1954..>2350
                     /locus_tag="PLF56_13150"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_013162075.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="HAD family hydrolase"
                     /protein_id="HOQ54544.1"
                     /translation="MSSDVLRGPTEFYDAYVFDMDGTIYLGDHLLPGAARLINELRRR
                     ELPIRYLSNNPTKDPEMYARKLTKLGLPTPVEDISNTIVTTTKWLLANHPDAVLFPIS
                     EEPLKRALVKAGFTLSDDPEEIDIVIASYD"
BASE COUNT          416 a          783 c          694 g          457 t
ORIGIN      
        1 gtgacctgca gtgcttccac ccctacgagg actgtgtgca ggtgaagcgg gcaatgatga
       61 cctcggcggc ccaatcgatc ctgctgctgg atcactccaa actcaaccgg cgcgcgttgc
      121 acaccttcgc ggcgatgacc gacttcgatc aggtggtggt ggactccctc acccccgaat
      181 cactggtgag cgacctgcgc gaacgcggcg tcagcgtcgt ccgggcagag gccccggcgt
      241 cctgaagcga cgctcgtccg gacgcgactc gtctttgaca tcctcacaaa ctctgttaca
      301 aaccccttgc ccactggtag gttcatgcca ggcgggccct gtcggcggtc ccgccaagct
      361 cgtgcaaagg agtacgaatg ggtctgcgcg tcgtggtcgc ggctgatacg gccggatatg
      421 actacaagga gcaactgaag aaggacctcg aggccgacaa ccgtgtcgat gaagtcatcg
      481 acgttggcgt cgggtccggt gaggacgtcg actaccccca cgttgcggtg gcgggtgcga
      541 gactgatcgc agacggcaag gctgatcgcg gcctgttcgt gtgcggcacg ggcatgggcg
      601 tcgcgatcgc ggcgaacaag gtgcccggca tcagggcgag tgtggctcac gacagctttt
      661 ccgtcgagcg actcgtgttg tccaacaacg cccaggttct cactttcggt cagcgcgtca
      721 tcgggctcga gttggctcgt cggctcgcga aggagtggct cggctacacc ttcgacccct
      781 catcgtcctc tgcacccaag gtggccgcgc tcgaatcgta cgacaagggc gacaacggca
      841 acgaagccgt acagaccagt tgcgactgag cagctgggtt attttccatc atcacatctg
      901 acaaggacgg cacatgttca ccagtgacat tgcaattttc ttgctcggcc tgatcgcagc
      961 catcttcggc gggtacttcg gtgcagccat cggtggcaac ttcgcgttcg cgctgaccgg
     1021 cttcatgatt ctcgtttcct ggggcatttt cgtgggctcc ggaagcgata tcggcttcaa
     1081 ctatctggcc ttcggccccg tcatgggccc gcacatcacg ttcgccgccg gctgtgccgc
     1141 agctgcgtat gcgatgcaca aaggtctgat cgaatcagga cgggacgcca cctcaccgct
     1201 ggcgcgcctc ggccgggtgg acgtgttgct cgtcggcgct gccttcggtg cgttcggcta
     1261 cctgttcaac atcggcctca gcttcatccc gtggttcggc agccacatcg actcggtcgc
     1321 actgaccgtg ttcaccagca acgtgctcgc ccggctgatt tggggcaaca gcctgatggt
     1381 gacccagaag tacaacaagg gcgccacgaa cttcttcggc aagatcgccc cgaacgagac
     1441 cgacttctgg ctgcgctacc aggagaagcc cagccagtac ctgccgctcg gcatcttcgc
     1501 cggcggtctg gctgccggtg ccggaatcat gctgggtcac ttcgttcccg gtggggcagc
     1561 gtttgcgcac accctgccgt tcgccatctc ggccatcatc atcctgttcc tgatccttgg
     1621 cgcagagatg ccggtgcagc accacatcac gatcatcggt gcgctcgccg cggtgaagtt
     1681 catgccggtg ctcgcgggtg ccggtttcga atggaacggg gtgtggacct cggccacctg
     1741 gatgaccgcc atcggcgcca ttctgatcgg ctgtgtgttc gccatgatct cggccttcct
     1801 cgccgagttc cagtcgcagt tgttccatca gcgtggcaac acccacatcg acccgccggc
     1861 ggccgccatc tggagcacga cgctgttggt gctggcgatc tccggcgcac tgacccacgc
     1921 ctgacccgcc tctccgcctg aaaggtgacc gacatgtcga gcgacgtact gcgtggaccc
     1981 accgagttct acgacgccta cgtcttcgac atggacggca ccatctacct gggtgaccac
     2041 ctcctgcccg gcgcagctcg cctgatcaat gagctgcgcc ggcgggagtt gcccattcgc
     2101 tacctctcga acaaccccac caaagacccc gagatgtacg cccgtaagct gaccaagctc
     2161 gggctgccca ccccggtcga agacatctcg aacaccatcg tcaccaccac caagtggctg
     2221 ctggcgaacc accccgacgc cgtactgttt ccgatcagcg aagagccgct caagcgtgcc
     2281 ctggtcaagg ccggcttcac gctcagcgac gatcccgaag agatcgacat cgtgatcgcc
     2341 agctacgacc
//
LOCUS       DAOSUG010000491         2344 bp    DNA     linear   ENV 10-JUN-2024
DEFINITION  MAG TPA_asm: Micropruina sp. isolate AMR_MDS_2195
            NODE_18424_length_2344_cov_6.253386, whole genome shotgun sequence.
ACCESSION   DAOSUG010000491 DAOSUG010000000
VERSION     DAOSUG010000491.1
DBLINK      BioProject: PRJNA925477
            BioSample: SAMN32864262
            Sequence Read Archive: SRR4236673
KEYWORDS    WGS; ENV; Metagenome Assembled Genome; MAG; Third Party Data; TPA;
            TPA:assembly.
SOURCE      Micropruina sp. (wastewater metagenome)
  ORGANISM  Micropruina sp.
            Bacteria; Bacillati; Actinomycetota; Actinomycetes;
            Propionibacteriales; Nocardioidaceae; Micropruina.
REFERENCE   1  (bases 1 to 2344)
  AUTHORS   Abdulkadir,N., Saraiva,J.P., Zhang,J., Stolte,S., Gillor,O.,
            Harms,H. and Rocha,U.
  TITLE     Genome-centric analyses of 165 metagenomes show that mobile genetic
            elements are crucial for the transmission of antimicrobial
            resistance genes to pathogens in activated sludge and wastewater
  JOURNAL   Microbiol Spectr 12 (3), e0291823 (2024)
   PUBMED   38289113
REFERENCE   2  (bases 1 to 2344)
  AUTHORS   Abdulkadir,N.
  TITLE     Direct Submission
  JOURNAL   Submitted (24-MAR-2023) Environmental Microbiology, Helmholtz
            Center for Environmental Research -UFZ, Permoserstr 15, Leipzig
            04318, Germany
COMMENT     The annotation was added by the NCBI Prokaryotic Genome Annotation
            Pipeline (PGAP). Information about PGAP can be found here:
            https://www.ncbi.nlm.nih.gov/genome/annotation_prok/
            
            ##Genome-Assembly-Data-START##
            Assembly Method        :: SPAdes v. 3.11.1
            Genome Representation  :: Full
            Expected Final Version :: Yes
            Genome Coverage        :: Not Applicable
            Sequencing Technology  :: Illumina MiSeq
            ##Genome-Assembly-Data-END##
            
            ##Genome-Annotation-Data-START##
            Annotation Provider               :: NCBI
            Annotation Date                   :: 05/04/2023 04:57:10
            Annotation Pipeline               :: NCBI Prokaryotic Genome
                                                 Annotation Pipeline (PGAP)
            Annotation Method                 :: Best-placed reference protein
                                                 set; GeneMarkS-2+
            Annotation Software revision      :: 6.5
            Features Annotated                :: Gene; CDS; rRNA; tRNA; ncRNA
            Genes (total)                     :: 2,776
            CDSs (total)                      :: 2,750
            Genes (coding)                    :: 2,714
            CDSs (with protein)               :: 2,714
            Genes (RNA)                       :: 26
            tRNAs                             :: 24
            ncRNAs                            :: 2
            Pseudo Genes (total)              :: 36
            CDSs (without protein)            :: 36
            Pseudo Genes (ambiguous residues) :: 0 of 36
            Pseudo Genes (frameshifted)       :: 2 of 36
            Pseudo Genes (incomplete)         :: 32 of 36
            Pseudo Genes (internal stop)      :: 2 of 36
            ##Genome-Annotation-Data-END##
FEATURES             Location/Qualifiers
     source          1..2344
                     /organism="Micropruina sp."
                     /mol_type="genomic DNA"
                     /submitter_seqid="NODE_18424_length_2344_cov_6.253386"
                     /isolate="AMR_MDS_2195"
                     /isolation_source="Activated sludge of municipal
                     wastewater treatment plants"
                     /db_xref="taxon:2737536"
                     /environmental_sample
                     /geo_loc_name="Canada"
                     /lat_lon="49.25 N 123.1 W"
                     /collection_date="2015-06-16"
                     /metagenome_source="wastewater metagenome"
                     /note="metagenomic"
     gene            complement(<1..211)
                     /locus_tag="PLF56_13155"
     CDS             complement(<1..211)
                     /locus_tag="PLF56_13155"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_014167065.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="50S ribosomal protein L7/L12"
                     /protein_id="HOQ54545.1"
                     /translation="MAKLSTDDLLEAFKEMTLIELSEFVKQFEDTFGVTAAAPVAAAA
                     APTASGAGGDAAEEEEVSNEVDVILE"
     gene            complement(230..817)
                     /gene="rplJ"
                     /locus_tag="PLF56_13160"
     CDS             complement(230..817)
                     /gene="rplJ"
                     /locus_tag="PLF56_13160"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_020388132.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_component: GO:0015934 - large ribosomal subunit
                     [Evidence IEA];
                     GO_function: GO:0003735 - structural constituent of
                     ribosome [Evidence IEA];
                     GO_process: GO:0006412 - translation [Evidence IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="50S ribosomal protein L10"
                     /protein_id="HOQ54546.1"
                     /translation="MARADKATAVGELKDSFTNSAATVLTEYRGLTVKSLQELRRSLG
                     ENATYAVAKNTLTTIAAREAGIEGLESQLSGPTAITFISGDVATVAKGLRDFAKANPH
                     LVIKGGVMDGRVLDADGVKKLADLESREVLLAKLAGAMNGNLAKAAGLFAAPLSQAAR
                     VLAALTDQKQAGATPATDAAPAAEATDDAAEAASE"
     gene            complement(1172..1873)
                     /gene="rplA"
                     /locus_tag="PLF56_13165"
     CDS             complement(1172..1873)
                     /gene="rplA"
                     /locus_tag="PLF56_13165"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_013160472.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_component: GO:0000311 - plastid large ribosomal subunit
                     [Evidence IEA];
                     GO_component: GO:0022625 - cytosolic large ribosomal
                     subunit [Evidence IEA];
                     GO_function: GO:0003735 - structural constituent of
                     ribosome [Evidence IEA];
                     GO_process: GO:0006412 - translation [Evidence IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="50S ribosomal protein L1"
                     /protein_id="HOQ54547.1"
                     /translation="MKRSKAYRAAAEKRDANQLYSAEEALGIVKDGASAKFDETVEVA
                     MRLGVDPRKADQMVRGTVNLPNGTGKTARVLVFATGDRAAEATAAGADEVGADDLIEK
                     ITGGYLDFDAVVATPDMMGKVGRLGRVLGPRGLMPNPKTGTVTMDVAKAVSDIKGGKI
                     EFRVDRHANLCFVVGKASFTPEQLSQNYFAALDEVLRLKPSASKGRYLRKVTVSSTMG
                     PGVSVDPTRVKPAED"
     gene            complement(2000..>2344)
                     /gene="rplK"
                     /locus_tag="PLF56_13170"
     CDS             complement(2000..>2344)
                     /gene="rplK"
                     /locus_tag="PLF56_13170"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_010058817.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_component: GO:0000315 - organellar large ribosomal
                     subunit [Evidence IEA];
                     GO_component: GO:0022625 - cytosolic large ribosomal
                     subunit [Evidence IEA];
                     GO_function: GO:0003735 - structural constituent of
                     ribosome [Evidence IEA];
                     GO_process: GO:0006412 - translation [Evidence IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="50S ribosomal protein L11"
                     /protein_id="HOQ54548.1"
                     /translation="LGPHGVNIMEFCKRYNAETEAMRGTIVPVEITIYEDRTFTFITK
                     TPPAAELIKKAAGIKSGSARPHTDKVGKITAAQVREIATTKLPDLNANDLEAAAKIVE
                     GTARSMGITVEG"
BASE COUNT          334 a          772 c          782 g          456 t
ORIGIN      
        1 actcgaggat cacgtcgacc tcgttgctga cctcttcttc ttcggcagcg tcgccaccgg
       61 cgccactggc ggtcggggcg gcagcggcgg ccaccggggc ggcagcggtc acgccgaagg
      121 tgtcttcgaa ctgcttcacg aactcggaga gctcgatcag ggtcatctct ttgaacgcct
      181 cgaggaggtc gtcagtgctg agcttcgcca tggtgtgatt cctttcgtct tactcggatg
      241 cagcttcagc cgcgtcgtcg gtggcctccg cagcaggcgc tgcatcggtt gccggggtgg
      301 ccccggcctg cttctggtcc gtcagggcgg ccaggacgcg agcggcctgg ctgagcgggg
      361 cggcgaacag gcccgcggcc ttcgccaggt tgccgttcat ggcacctgcg agcttggcga
      421 gcagaacctc gcgcgactcc aggtcagcca gcttcttgac cccgtcggcg tcgaggacgc
      481 gaccgtccat cacgccgccc ttgatgacca gatgcgggtt cgccttcgcg aaatcacgca
      541 gtcccttggc gacggttgcg acgtcgccag agatgaaggt gatcgcggtc ggaccggaga
      601 gttggctctc caggccctcg atgcccgcct cacgggctgc gatcgtcgtc agcgtgttct
      661 tcgcgacggc gtaggtggcg ttctcaccca gggacctgcg caactcctgc aggctcttca
      721 cggtgagacc gcggtactcg gtcagtacgg ttgcggccga gttggtgaag ctgtccttca
      781 gctcgccaac ggccgtggcc ttgtctgccc tcgccatgag tctccttccc acttccgtcg
      841 atgccgacga ccacatggaa aaaccccggc gcgcatgcac tccggggcga actctgacga
      901 gctgttcagg aatacctgcg cgggcgatca ttgatcctta ggacaccatg aggtgtcacc
      961 agcggtcttc ggcttcgtcc atgctacgac atggctggtg aagcagccaa atccacgccg
     1021 agccagggac gtcccgcgaa cgcccgccca agtatcgtgc gcccgcgaag cttcgcgggc
     1081 cctggaaagt taggggggcg ttcgcggacg ggcccacaac agcgaacggc ccggcccccc
     1141 atcgggagcc gggccgaacg ccgtacagtg ctcagtcctc ggcgggcttc acgcgcgtcg
     1201 ggtcgacgct gacgcccgga cccatcgtgg acgacacggt gaccttgcgc aggtaccgcc
     1261 ccttggatgc ggacggcttg agccgcagca cctcgtccag cgctgcgaag tagttctggc
     1321 tcagctgctc gggagtgaag gacgccttgc cgaccacgaa acacagattg gcgtgccgat
     1381 cgacccggaa ctcgatcttg ccgcccttga tgtcgctgac cgccttggcc acgtccatgg
     1441 tgacggtgcc ggtcttcggg ttgggcatca ggccgcgcgg gccgagcacc cggccgagcc
     1501 ggccgacctt gcccatcatg tcgggcgtgg ccaccacggc gtcgaagtcg aggtaacccc
     1561 cggtgatctt ctcgatcaga tcgtcggcgc ccacttcgtc cgcgccggct gcagtcgcct
     1621 cggccgcacg atcaccggtg gcgaagacga ggacccttgc cgtcttgccg gtgccgttgg
     1681 gcagattgac ggtgccacga accatttggt cggccttgcg ggggtcgacc cccaggcgca
     1741 tggccacctc gacggtctcg tcgaacttgg cggaggcacc gtccttgacg atgccgagcg
     1801 cctcttcggc gctgtacagc tggttggcgt cgcgcttctc ggcggcggcg cggtaggcct
     1861 tgcttctctt cattctggtc tccttgtgat tccggcctcg tgtggccggg ttgccagttg
     1921 cggtacggac ggtcgtcctg ccgcgattgc gttgttccga ggtctcgacc aacgaacacc
     1981 ggtctcgacc ggcggtggat cagccctcga cggtgatgcc catggagcgg gcggtgccct
     2041 cgacgatctt ggccgcggcc tcgaggtcgt tggcgttcag gtcgggcagc ttggtggtgg
     2101 cgatctcgcg cacctgggcg gcggtgatct tgccgacctt gtcggtgtgc ggacgagctg
     2161 aaccgctctt gatgccggcg gccttcttga tcagctcggc ggccggcggc gtcttggtga
     2221 tgaacgtgaa cgtgcggtct tcatagatcg tgatctcgac cggcacgatc gtgccgcgca
     2281 tggcctcggt ttctgcgttg taccgcttgc agaactccat gatgttgacg ccgtgcgggc
     2341 cgag
//
LOCUS       DAOSUG010000492         2342 bp    DNA     linear   ENV 10-JUN-2024
DEFINITION  MAG TPA_asm: Micropruina sp. isolate AMR_MDS_2195
            NODE_18459_length_2342_cov_3.037167, whole genome shotgun sequence.
ACCESSION   DAOSUG010000492 DAOSUG010000000
VERSION     DAOSUG010000492.1
DBLINK      BioProject: PRJNA925477
            BioSample: SAMN32864262
            Sequence Read Archive: SRR4236673
KEYWORDS    WGS; ENV; Metagenome Assembled Genome; MAG; Third Party Data; TPA;
            TPA:assembly.
SOURCE      Micropruina sp. (wastewater metagenome)
  ORGANISM  Micropruina sp.
            Bacteria; Bacillati; Actinomycetota; Actinomycetes;
            Propionibacteriales; Nocardioidaceae; Micropruina.
REFERENCE   1  (bases 1 to 2342)
  AUTHORS   Abdulkadir,N., Saraiva,J.P., Zhang,J., Stolte,S., Gillor,O.,
            Harms,H. and Rocha,U.
  TITLE     Genome-centric analyses of 165 metagenomes show that mobile genetic
            elements are crucial for the transmission of antimicrobial
            resistance genes to pathogens in activated sludge and wastewater
  JOURNAL   Microbiol Spectr 12 (3), e0291823 (2024)
   PUBMED   38289113
REFERENCE   2  (bases 1 to 2342)
  AUTHORS   Abdulkadir,N.
  TITLE     Direct Submission
  JOURNAL   Submitted (24-MAR-2023) Environmental Microbiology, Helmholtz
            Center for Environmental Research -UFZ, Permoserstr 15, Leipzig
            04318, Germany
COMMENT     The annotation was added by the NCBI Prokaryotic Genome Annotation
            Pipeline (PGAP). Information about PGAP can be found here:
            https://www.ncbi.nlm.nih.gov/genome/annotation_prok/
            
            ##Genome-Assembly-Data-START##
            Assembly Method        :: SPAdes v. 3.11.1
            Genome Representation  :: Full
            Expected Final Version :: Yes
            Genome Coverage        :: Not Applicable
            Sequencing Technology  :: Illumina MiSeq
            ##Genome-Assembly-Data-END##
            
            ##Genome-Annotation-Data-START##
            Annotation Provider               :: NCBI
            Annotation Date                   :: 05/04/2023 04:57:10
            Annotation Pipeline               :: NCBI Prokaryotic Genome
                                                 Annotation Pipeline (PGAP)
            Annotation Method                 :: Best-placed reference protein
                                                 set; GeneMarkS-2+
            Annotation Software revision      :: 6.5
            Features Annotated                :: Gene; CDS; rRNA; tRNA; ncRNA
            Genes (total)                     :: 2,776
            CDSs (total)                      :: 2,750
            Genes (coding)                    :: 2,714
            CDSs (with protein)               :: 2,714
            Genes (RNA)                       :: 26
            tRNAs                             :: 24
            ncRNAs                            :: 2
            Pseudo Genes (total)              :: 36
            CDSs (without protein)            :: 36
            Pseudo Genes (ambiguous residues) :: 0 of 36
            Pseudo Genes (frameshifted)       :: 2 of 36
            Pseudo Genes (incomplete)         :: 32 of 36
            Pseudo Genes (internal stop)      :: 2 of 36
            ##Genome-Annotation-Data-END##
FEATURES             Location/Qualifiers
     source          1..2342
                     /organism="Micropruina sp."
                     /mol_type="genomic DNA"
                     /submitter_seqid="NODE_18459_length_2342_cov_3.037167"
                     /isolate="AMR_MDS_2195"
                     /isolation_source="Activated sludge of municipal
                     wastewater treatment plants"
                     /db_xref="taxon:2737536"
                     /environmental_sample
                     /geo_loc_name="Canada"
                     /lat_lon="49.25 N 123.1 W"
                     /collection_date="2015-06-16"
                     /metagenome_source="wastewater metagenome"
                     /note="metagenomic"
     gene            complement(<1..344)
                     /locus_tag="PLF56_13175"
     CDS             complement(<1..344)
                     /locus_tag="PLF56_13175"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_013861726.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_function: GO:0016903 - oxidoreductase activity, acting
                     on the aldehyde or oxo group of donors [Evidence IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="2-oxoacid:acceptor oxidoreductase family
                     protein"
                     /protein_id="HOQ54549.1"
                     /translation="MSEVRNVDRVVIRFAGDSGDGMQLTGDRFTSDTAQLGNDFSTLP
                     NFPAEIRAPQGTVAGVSSFQVHFADFDIRTPGDRPDVLVAMNPAALKANLHDLPRGAV
                     ILADTADFTTRNL"
     gene            complement(406..1134)
                     /locus_tag="PLF56_13180"
     CDS             complement(406..1134)
                     /locus_tag="PLF56_13180"
                     /inference="COORDINATES: protein motif:HMM:NF014016.2"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_function: GO:0016829 - lyase activity [Evidence IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="CbiX/SirB N-terminal domain-containing protein"
                     /protein_id="HOQ54550.1"
                     /translation="MGRASMTAARSGVRPLVLAVHGTRSAEGTATSQALATAVAERLD
                     VETHLGWVDIHTPTLTDTLRRLGEAIVVPVFLTSGYHVTSDIPAAIAAAGGRAMATPL
                     VGADVLPAVLDRLAAVGDYDAVVLGSAGSLRARALTQVRAVTSALAAAVGRPAATGYV
                     TAASPTVPEAVAALRAAGHARVAIASYLLAPGRFSVALERAGADAVSRPIGVHPLLIH
                     LIEQRWRAADAQPLTAFQPKAVND"
     gene            complement(1179..>2342)
                     /locus_tag="PLF56_13185"
     CDS             complement(1179..>2342)
                     /locus_tag="PLF56_13185"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_013162001.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_function: GO:0003924 - GTPase activity [Evidence IEA];
                     GO_function: GO:0005525 - GTP binding [Evidence IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="GTP-binding protein"
                     /protein_id="HOQ54551.1"
                     /translation="LGIVDLALLTDGLRAEREQGITIDVAYRYFATAKRTFVLADCPG
                     HVQYTRNTVTGSSTADVLVLLVDARKGVLEQTRRHLAVAALLRVPHLVFAVNKIDLVD
                     FDQARYEAVAAEIADVAAGLGISDVRCVPVSALLGDNIVDRSERTPWYDGPSMLELLK
                     ALPSGTPVEDEPFRFPVQLTIRPQAAARDPQHVEYRGYAGQIASGVVRVGDAVTVLPT
                     GRQTTVTGIDDAGDELTEAFAPMSVTLRLADEVDITRGELIAGAADAPTPTQDVDGLV
                     CWLSDQALRPGSRVLLKHTTRTVQGIVKEIKGRLDLDTLAPVEAEALTLNDIGRVTIR
                     LAKPIVAEDYGTSRRTGAFLLVDPQMGSTLAAGMIRTALGADEFEAETLNWSI"
BASE COUNT          341 a          796 c          866 g          339 t
ORIGIN      
        1 aggttgcggg tggtgaagtc ggcggtgtcg gccaggatca ccgcgccccg cggcaggtcg
       61 tgcaggttgg ccttcagtgc ggccgggttc atcgccacca gcacgtccgg acgatcgccc
      121 ggggtgcgaa tgtcgaagtc ggcgaagtgc acctgaaagc tggaaactcc ggccacggtg
      181 ccctgcggcg cgcggatctc ggccggaaag ttcggcaggg tggagaagtc gttgcccagc
      241 tgggcggtgt cggaggtgaa ccggtcaccg gtcagttgca tgccgtcgcc ggagtcaccg
      301 gcgaagcgga tcacgacccg gtcgacgttg cgaacctcgc tcatgcgggt tgcgccctcc
      361 ttcgattgtg gcaacccgag cgagtgtagt tgttggtcga gcacgtcagt cattcacagc
      421 tttcggttgg aacgcggtca ggggttgcgc gtcggcggcg cgccagcgct gttcgatcag
      481 gtggatcagc agcgggtgga cgccgatcgg ccggctcacc gcgtcggcgc cggcccgctc
      541 cagggccacc gagaaccgtc cgggtgccag caggtacgag gcgatcgcca cccgcgcgtg
      601 cccggcggcg cgcagcgcgg ccaccgcctc gggaacggtg ggtgaggccg ccgtcacata
      661 gcccgttgct gccggacgtc ccaccgcggc cgccagcgcc gaggtgacgg cacgcacctg
      721 tgtcagggcc cgggcgcgca gtgacccggc gctgcccagc accaccgcgt cgtagtcgcc
      781 gaccgcggcc agccggtcga gcacggccgg cagcacgtcg gcaccgacca gcggggtcgc
      841 catggctcgt ccgccggctg cagcgatcgc ggccggaatg tcagaggtga cgtggtaacc
      901 gctggtcagg aaaaccggca ccacgatcgc ctcgcccagt cggcgcaggg tgtcggtgag
      961 ggtgggggtg tgaatgtcga cccagccgag gtgcgtctcg acgtccagcc gctcggcgac
     1021 ggctgtcgcc aaggcctggg aggtcgccgt gccctcggca ctacgggtgc cgtgcaccgc
     1081 caacaccagc ggacggacgc ccgagcgcgc agcggtcatc gaggcccggc ccatcggagc
     1141 agggtccgtg aaggcagcgt ccgtcggagc agggcccatc agatcgacca gttcagggtc
     1201 tcggcctcga actcgtccgc gcccagggcg gtgcggatca tgccggcggc gagcgtgctg
     1261 cccatttggg gatcgaccag cagaaacgcg ccggtgcggc gcgaggtgcc gtagtcctcg
     1321 gccacgatcg gtttggccag ccggatggtc acccggccga tgtcgttcag ggtcagcgcc
     1381 tcggcctcga ccggcgccaa agtgtccaga tcgaggcggc ccttgatctc tttcacgatg
     1441 ccctgcacgg tgcgggtggt gtgcttgagc agcacccgcg agcccggtcg cagcgcctgg
     1501 tccgacagcc agcagaccag cccgtccacg tcctgggtgg gggtgggggc gtccgccgca
     1561 ccggcgatca gctcgccgcg ggtgatgtcg acctcgtcgg ccagccgcag cgtcaccgac
     1621 atcggcgcga acgcctcggt cagctcgtca cccgcgtcgt cgatgccggt cacggtggtc
     1681 tgcctgccgg tgggcagcac ggtcaccgcg tcgcccaccc gcaccacgcc cgaagcgatc
     1741 tggcccgcgt agccgcggta ctcgacgtgc tgcgggtcgc gcgcggcagc ctgtggccgg
     1801 atggtcagct gcaccggaaa ccggaacggc tcgtcctcga ccggggtgcc ggacggcagc
     1861 gccttcagga gttccagcat gctcggcccg tcgtaccagg gcgtgcgctc ggagcggtcg
     1921 acgatgttgt cgccgagcag cgccgagacg ggtacgcagc gcacatccga gatgcccagc
     1981 ccggcagcca cgtcggcgat ctcggcggcg accgcctcgt aacgggcctg gtcgaagtcg
     2041 accaggtcga tcttgttgac cgcgaacacc aggtgcggca cccgcagcag tgccgcgacg
     2101 gccaggtgcc ggcgggtctg ctcgagcacg cccttgcgcg cgtccaccag gagcaccagc
     2161 acgtcggcgg tggacgagcc ggtcaccgtg ttgcgggtgt actgcacgtg cccggggcag
     2221 tcggccagca cgaaggtgcg cttggcggtg gcgaagtagc ggtaggcgac gtcgatggtg
     2281 atgccctgct cacgttcggc gcgcaggccg tcggtgagca gcgccaggtc gacgatgccg
     2341 ag
//
LOCUS       DAOSUG010000493         2336 bp    DNA     linear   ENV 10-JUN-2024
DEFINITION  MAG TPA_asm: Micropruina sp. isolate AMR_MDS_2195
            NODE_18523_length_2336_cov_3.041648, whole genome shotgun sequence.
ACCESSION   DAOSUG010000493 DAOSUG010000000
VERSION     DAOSUG010000493.1
DBLINK      BioProject: PRJNA925477
            BioSample: SAMN32864262
            Sequence Read Archive: SRR4236673
KEYWORDS    WGS; ENV; Metagenome Assembled Genome; MAG; Third Party Data; TPA;
            TPA:assembly.
SOURCE      Micropruina sp. (wastewater metagenome)
  ORGANISM  Micropruina sp.
            Bacteria; Bacillati; Actinomycetota; Actinomycetes;
            Propionibacteriales; Nocardioidaceae; Micropruina.
REFERENCE   1  (bases 1 to 2336)
  AUTHORS   Abdulkadir,N., Saraiva,J.P., Zhang,J., Stolte,S., Gillor,O.,
            Harms,H. and Rocha,U.
  TITLE     Genome-centric analyses of 165 metagenomes show that mobile genetic
            elements are crucial for the transmission of antimicrobial
            resistance genes to pathogens in activated sludge and wastewater
  JOURNAL   Microbiol Spectr 12 (3), e0291823 (2024)
   PUBMED   38289113
REFERENCE   2  (bases 1 to 2336)
  AUTHORS   Abdulkadir,N.
  TITLE     Direct Submission
  JOURNAL   Submitted (24-MAR-2023) Environmental Microbiology, Helmholtz
            Center for Environmental Research -UFZ, Permoserstr 15, Leipzig
            04318, Germany
COMMENT     The annotation was added by the NCBI Prokaryotic Genome Annotation
            Pipeline (PGAP). Information about PGAP can be found here:
            https://www.ncbi.nlm.nih.gov/genome/annotation_prok/
            
            ##Genome-Assembly-Data-START##
            Assembly Method        :: SPAdes v. 3.11.1
            Genome Representation  :: Full
            Expected Final Version :: Yes
            Genome Coverage        :: Not Applicable
            Sequencing Technology  :: Illumina MiSeq
            ##Genome-Assembly-Data-END##
            
            ##Genome-Annotation-Data-START##
            Annotation Provider               :: NCBI
            Annotation Date                   :: 05/04/2023 04:57:10
            Annotation Pipeline               :: NCBI Prokaryotic Genome
                                                 Annotation Pipeline (PGAP)
            Annotation Method                 :: Best-placed reference protein
                                                 set; GeneMarkS-2+
            Annotation Software revision      :: 6.5
            Features Annotated                :: Gene; CDS; rRNA; tRNA; ncRNA
            Genes (total)                     :: 2,776
            CDSs (total)                      :: 2,750
            Genes (coding)                    :: 2,714
            CDSs (with protein)               :: 2,714
            Genes (RNA)                       :: 26
            tRNAs                             :: 24
            ncRNAs                            :: 2
            Pseudo Genes (total)              :: 36
            CDSs (without protein)            :: 36
            Pseudo Genes (ambiguous residues) :: 0 of 36
            Pseudo Genes (frameshifted)       :: 2 of 36
            Pseudo Genes (incomplete)         :: 32 of 36
            Pseudo Genes (internal stop)      :: 2 of 36
            ##Genome-Annotation-Data-END##
FEATURES             Location/Qualifiers
     source          1..2336
                     /organism="Micropruina sp."
                     /mol_type="genomic DNA"
                     /submitter_seqid="NODE_18523_length_2336_cov_3.041648"
                     /isolate="AMR_MDS_2195"
                     /isolation_source="Activated sludge of municipal
                     wastewater treatment plants"
                     /db_xref="taxon:2737536"
                     /environmental_sample
                     /geo_loc_name="Canada"
                     /lat_lon="49.25 N 123.1 W"
                     /collection_date="2015-06-16"
                     /metagenome_source="wastewater metagenome"
                     /note="metagenomic"
     gene            complement(316..1167)
                     /locus_tag="PLF56_13190"
     CDS             complement(316..1167)
                     /locus_tag="PLF56_13190"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_015069576.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="class II fructose-bisphosphate aldolase"
                     /protein_id="HOQ54552.1"
                     /translation="MLIGLREALDYAETNNCGIAAINTPTLELLLGTVKVAERYNVPL
                     VVQHAQVHEPVNSIEDIGPVMVELARRSSAPIVVHVDHGETYEYVERGFAVGFNSAMF
                     DGSRLPYDDNVEQTRAVVRLANERGFGVEGELGIMPGREDGVESTGTADETLYTSPED
                     AAAFVRATGVTALACSFGTVHGLYKAEPKINYELIGRLREASGVPIVMHGGSGLSCEE
                     YRASIDQGVRKINYYTYADKAALEAARALLADKPATYVFSDLTVVAQAAIEKDIDALV
                     RCLYRVA"
     gene            1588..>2336
                     /locus_tag="PLF56_13195"
     CDS             1588..>2336
                     /locus_tag="PLF56_13195"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_016669731.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_function: GO:0003824 - catalytic activity [Evidence
                     IEA];
                     GO_function: GO:0051536 - iron-sulfur cluster binding
                     [Evidence IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="radical SAM protein"
                     /protein_id="HOQ54553.1"
                     /translation="MAHGHPSGIGSVKPVNWAYQRAPMIVYWELTNACGLACRHCRAT
                     AMPDPAPGELTTAEAIALLDDITGFGTPLPHLVMTGGDPLMRPDLRELIAAATARGIG
                     VSLAPAVTPLLTRERMHEFAADGVTAISLSLDGSCPELHDGLRGVPGTFDATMVALDQ
                     AAEAGLAVQVNTLVTASTADNLPAIYELLKSHTLMQWSLFFLISIGRGAELTELSPGA
                     AEKVLLWAQRTARTAPWRIKTTEAMHYRRLSA"
BASE COUNT          371 a          847 c          738 g          380 t
ORIGIN      
        1 aaatgtcgcc accagtcggg gtgtggggcc gcgacacgcc gggtctgggc cgcactcctg
       61 cgggtgatgt caggtcaccc ggcgccgagc acgtgaacgg ccggagcact tggtcggcgt
      121 caacgtgacc aatcggtggt cggcgtatcg agacccccac ctgacgcggt tcccgcccaa
      181 tcgctgggcg aaccggtcga gacccaccct gacgcgatcc ttgtcacgct cgaccgacgg
      241 atggtgcgtt gatcagcacg tcagcgtggc cgggaccggc tggccgcgcc cggcgtgtgc
      301 cccgagcggc cgaggtcagg cgacccggta caggcagcgc accagcgcgt cgatgtcctt
      361 ctcgatggca gcctgcgcca ccaccgtgag gtccgagaac acatacgtcg cgggcttgtc
      421 ggccagtagc gcgcgggcag cctcgagcgc ggccttgtcg gcgtaggtgt agtagttgat
      481 cttgcggacg ccctggtcaa tggaggcccg gtactcctcg caactcagcc ccgacccccc
      541 gtgcatcacg atcggcacac cggacgcctc gcgcagccgg ccgatcagct cgtagttgat
      601 cttcggctcg gccttgtaca ggccgtgcac cgtgccgaac gagcacgcca gcgccgtcac
      661 gccggtggcc cggacgaacg ccgcggcgtc ctcaggtgag gtgtacaggg tctcgtcggc
      721 ggtgccggtg gattcgacgc cgtcctcgcg gccgggcatg atgccgagct caccctcgac
      781 gccgaagccc cgttcattcg ccaaccggac cactgcgcgg gtctgttcga cgttgtcgtc
      841 gtagggcagc cgggacccgt cgaacatggc tgagttgaag ccgacggcga agccccgctc
      901 gacgtactcg taggtctcgc cgtgatcgac gtgcaccacg atcggggcgc tcgagcggcg
      961 ggccagttcg accatcaccg gaccgatgtc ttcgatcgag ttgaccggct catgcacctg
     1021 cgcatgctgg acgacgagtg gcacgttgta gcgctccgcg accttcaccg tgccgagcag
     1081 cagctcaagg gtcggggtgt tgatagcggc gattccgcag ttgttcgttt cggcgtagtc
     1141 gagcgcctcg cgcaacccga tcagcatgct cagtcctctc gttgccggtg cgaccgagcc
     1201 ggcgacgctg ccgactcggc gttctcgact ctaggggatt cccacatcag cggccgccac
     1261 tttgtggatt gtctcgacat gttcatacaa gttgacaagg ccagcggacg tgcccaccga
     1321 ctcgtggcgg caggtgcaga cacgcggtct tccggtcgcc gaaggtggct acggcttcgc
     1381 gcgtcggtgc cggcggccgc tcgccgcctg tctggtgagt ggggtcgacc acgctgccct
     1441 catgtgcctg gcctccacac gggcctccgt ccacacccag cgccccgcaa gtaaccactc
     1501 ccgccggcga ttgcccgaat cccccgcgcg tccgaagtcg cgccgttagg ctcgaatggt
     1561 ccgaccacga aaggatgagt ttctcccatg gctcacggcc acccgagcgg catcggctcc
     1621 gtcaaaccag tcaactgggc ctatcaacgg gcaccgatga tcgtctattg ggaactcacc
     1681 aacgcctgtg gcctggcctg ccgacactgt cgagccaccg ccatgcccga ccccgcgccc
     1741 ggtgaactga ccacggccga ggcgatcgcc ctgctcgacg acatcaccgg cttcggcacc
     1801 cccctgccgc atctggtgat gaccggcggc gacccgctga tgcgtccaga cctgcgcgaa
     1861 ctcatcgcgg cggccaccgc ccgcggcatc ggcgtctcac tcgctccggc cgtcaccccg
     1921 ctactcaccc gcgagcgcat gcacgagttc gccgccgacg gcgtaaccgc gatctcgttg
     1981 agcctggacg gctcgtgccc cgaactgcac gacgggctgc gcggcgtccc gggcaccttc
     2041 gatgccacca tggtcgccct cgaccaggcc gccgaagccg gtctcgcggt gcaggtcaac
     2101 acgctggtca cggccagcac cgccgacaac ctgccggcca tctacgaact gctcaaatcc
     2161 cacacgctga tgcagtggag cctgttcttt ctgatctcga tcggacgcgg cgccgaactc
     2221 accgaactca gtcccggtgc cgccgagaag gtgctgctgt gggcgcagcg caccgcccgc
     2281 accgcaccct ggcggatcaa aaccaccgag gccatgcact accggcgact gagcgc
//
LOCUS       DAOSUG010000494         2309 bp    DNA     linear   ENV 10-JUN-2024
DEFINITION  MAG TPA_asm: Micropruina sp. isolate AMR_MDS_2195
            NODE_18819_length_2309_cov_2.807453, whole genome shotgun sequence.
ACCESSION   DAOSUG010000494 DAOSUG010000000
VERSION     DAOSUG010000494.1
DBLINK      BioProject: PRJNA925477
            BioSample: SAMN32864262
            Sequence Read Archive: SRR4236673
KEYWORDS    WGS; ENV; Metagenome Assembled Genome; MAG; Third Party Data; TPA;
            TPA:assembly.
SOURCE      Micropruina sp. (wastewater metagenome)
  ORGANISM  Micropruina sp.
            Bacteria; Bacillati; Actinomycetota; Actinomycetes;
            Propionibacteriales; Nocardioidaceae; Micropruina.
REFERENCE   1  (bases 1 to 2309)
  AUTHORS   Abdulkadir,N., Saraiva,J.P., Zhang,J., Stolte,S., Gillor,O.,
            Harms,H. and Rocha,U.
  TITLE     Genome-centric analyses of 165 metagenomes show that mobile genetic
            elements are crucial for the transmission of antimicrobial
            resistance genes to pathogens in activated sludge and wastewater
  JOURNAL   Microbiol Spectr 12 (3), e0291823 (2024)
   PUBMED   38289113
REFERENCE   2  (bases 1 to 2309)
  AUTHORS   Abdulkadir,N.
  TITLE     Direct Submission
  JOURNAL   Submitted (24-MAR-2023) Environmental Microbiology, Helmholtz
            Center for Environmental Research -UFZ, Permoserstr 15, Leipzig
            04318, Germany
COMMENT     The annotation was added by the NCBI Prokaryotic Genome Annotation
            Pipeline (PGAP). Information about PGAP can be found here:
            https://www.ncbi.nlm.nih.gov/genome/annotation_prok/
            
            ##Genome-Assembly-Data-START##
            Assembly Method        :: SPAdes v. 3.11.1
            Genome Representation  :: Full
            Expected Final Version :: Yes
            Genome Coverage        :: Not Applicable
            Sequencing Technology  :: Illumina MiSeq
            ##Genome-Assembly-Data-END##
            
            ##Genome-Annotation-Data-START##
            Annotation Provider               :: NCBI
            Annotation Date                   :: 05/04/2023 04:57:10
            Annotation Pipeline               :: NCBI Prokaryotic Genome
                                                 Annotation Pipeline (PGAP)
            Annotation Method                 :: Best-placed reference protein
                                                 set; GeneMarkS-2+
            Annotation Software revision      :: 6.5
            Features Annotated                :: Gene; CDS; rRNA; tRNA; ncRNA
            Genes (total)                     :: 2,776
            CDSs (total)                      :: 2,750
            Genes (coding)                    :: 2,714
            CDSs (with protein)               :: 2,714
            Genes (RNA)                       :: 26
            tRNAs                             :: 24
            ncRNAs                            :: 2
            Pseudo Genes (total)              :: 36
            CDSs (without protein)            :: 36
            Pseudo Genes (ambiguous residues) :: 0 of 36
            Pseudo Genes (frameshifted)       :: 2 of 36
            Pseudo Genes (incomplete)         :: 32 of 36
            Pseudo Genes (internal stop)      :: 2 of 36
            ##Genome-Annotation-Data-END##
FEATURES             Location/Qualifiers
     source          1..2309
                     /organism="Micropruina sp."
                     /mol_type="genomic DNA"
                     /submitter_seqid="NODE_18819_length_2309_cov_2.807453"
                     /isolate="AMR_MDS_2195"
                     /isolation_source="Activated sludge of municipal
                     wastewater treatment plants"
                     /db_xref="taxon:2737536"
                     /environmental_sample
                     /geo_loc_name="Canada"
                     /lat_lon="49.25 N 123.1 W"
                     /collection_date="2015-06-16"
                     /metagenome_source="wastewater metagenome"
                     /note="metagenomic"
     gene            <1..1160
                     /locus_tag="PLF56_13200"
     CDS             <1..1160
                     /locus_tag="PLF56_13200"
                     /inference="COORDINATES: protein motif:HMM:NF021134.2"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_function: GO:0016758 - hexosyltransferase activity
                     [Evidence IEA]"
                     /codon_start=3
                     /transl_table=11
                     /product="glycosyltransferase 87 family protein"
                     /protein_id="HOQ54554.1"
                     /translation="ALYAGGTTIPGGNFEPWAPAMIDLDVYRRTGDLVLRGQDFYNVQ
                     AWLPWIYPPFPALLAVPFALAPLAMAQMVWIAINVGCVMAMLYRLGLTGARLSLAATA
                     IVWLVEPVRETLGFGQLGVLLVTAAVLDSMPGPTVFRRRVLPEGWLTGVATAVKLTPA
                     VIAAYNFFAGKRKAGIVSFLTFLACTAVGFVVLWGPSVTYWGRLLSGDSGLNSGIVFK
                     TNQSVLGVWTRLFGEASRGGLVLAALVAVLGIVAAVLMHRAGEVSYALCLAGITSLLA
                     SPISWSHHYVWIVPFGVVLLLNQRLPDYLRVSGLFYSIWTAFAPFKLLPGDNNVELTY
                     APLHMLVDNFGVYLGVAILVASVVAAYTPWGRDRRRAQAALRGPDAVRASA"
     gene            1469..>2309
                     /locus_tag="PLF56_13205"
     CDS             1469..>2309
                     /locus_tag="PLF56_13205"
                     /inference="COORDINATES: protein motif:HMM:NF019139.2"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_process: GO:0006353 - DNA-templated transcription
                     termination [Evidence IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="Rho termination factor N-terminal
                     domain-containing protein"
                     /protein_id="HOQ54555.1"
                     /translation="MTDTIEATATTGSGKGLSGMLLPELKQLGSSLGLKGTGAMRKSQ
                     LIDAIRSAQSGGSAAARTDAAPSQQPEQQQRKRAARRPAGEPDDRPEPTAGDQSHSES
                     SPAQSSDAGQGSRNAAAASAEAAPQAASVGEGADDVAARLEALQGERGRRRRGRADNE
                     AEAASAPSDAAAQARTEASEPTIDSRPAQSNNGNNDRQQQSRDDDDFGTGRRSRRRRN
                     RDRQSNNNNRRGNNNRGQSASMAGLDAEPVVNDDDVLLNVSGILDVLDNYAFVRTSGY
                     LPGN"
BASE COUNT          353 a          808 c          783 g          365 t
ORIGIN      
        1 ccgccctgta cgcgggcggc acgaccattc ccggcggcaa cttcgagccg tgggcaccgg
       61 ccatgatcga cctggacgtc taccgccgca ccggtgacct ggtgttgcgc gggcaggact
      121 tctacaacgt ccaggcgtgg ctgccctgga tctacccgcc gttcccggcc ctgctcgcgg
      181 tgcccttcgc gctggcgccg ttggcgatgg cgcagatggt gtggatcgcg atcaacgtcg
      241 gctgcgtgat ggctatgctg taccggctcg gcctgaccgg cgcccggctc agcctggccg
      301 ccaccgccat cgtgtggctg gtcgaaccgg tgcgcgaaac cctgggcttc ggccagttgg
      361 gcgtgctgct ggtgacggcc gcggtgctcg actcgatgcc cgggcccacc gtgtttcgtc
      421 gccgggtgct gcccgaagga tggctgaccg gcgtggccac cgcggtcaag ctcaccccgg
      481 cggtgatcgc cgcctacaac ttcttcgccg gcaagcgcaa ggcaggcatt gtcagcttcc
      541 tgacctttct ggcgtgcaca gccgtcggct tcgtcgtgct ctggggaccg tcggtgacct
      601 actggggccg gttgctctcg ggcgattcag ggctcaacag cggcatcgtg ttcaagacca
      661 accagtcggt gctcggggtg tggacgcggt tgttcggcga agccagccgt ggcggactgg
      721 tgctggccgc gttggtggcc gtgctcggta tcgtggcagc ggtgctgatg cacagggcgg
      781 gtgaggtgag ctatgccctg tgcctggccg gcatcaccag cctgctggcc tcgccgatct
      841 cgtggtcgca ccattacgtg tggatcgtgc cgttcggggt cgtgctgctg ctcaaccagc
      901 ggctacccga ctacctgcgc gtcagcggcc tgttctattc gatctggacg gccttcgccc
      961 cgttcaagct gctacccggc gacaacaatg tggagttgac ctacgcgccg ttgcacatgc
     1021 tcgtcgacaa cttcggggtg tacctgggcg tcgccattct ggtggccagc gtggtggcgg
     1081 cctacacgcc gtggggccgt gaccgccggc gcgcacaagc ggcgctgcgc ggcccggacg
     1141 cggtccgcgc ttcagcctga cggccgcgcc gatgacggcg cagtggcagg tgagcgactc
     1201 cgctcgtggc gcaacacgtg gcgtggtgcc ccgatttgaa gcattcggcg cgcgcagtgc
     1261 tacagtgaaa acgcgctgca gtgcagccac tgcccccaac aggtcggggc ggacgatctc
     1321 actcgtcgaa ggctccttct cattcttgcg ccgctgtgtg ccaggtgaga tgtatccaga
     1381 gctgtgccgg agctagtcac gctcctccca cctgcttgtc cggacccagc cgaagaccac
     1441 gacgtccgga tgttccgaaa ggacccatgt gaccgacacc atcgaagcca ccgccaccac
     1501 gggtagcggc aaggggctga gcggcatgct gcttcccgaa ctgaagcagc tcggctcgtc
     1561 cctgggcctc aaaggcaccg gcgcgatgcg caagagccaa ctgatcgacg ccatccgctc
     1621 ggcccagtcc ggcggttcgg ccgcagcccg caccgatgcg gcgcccagcc agcagcccga
     1681 acagcagcag cgcaagcgtg ccgcgcgccg gcccgccggc gagcccgacg accgtccaga
     1741 gccgaccgcg ggtgaccaga gccacagcga atcctcgccc gcgcagagtt ccgacgccgg
     1801 ccagggctca cgcaacgcag cggctgcttc ggctgaagcc gccccgcagg cagcatcggt
     1861 cggcgagggc gccgacgatg tcgccgcgcg gctggaggct cttcagggcg agcgcggccg
     1921 gcgcaggcgc ggtcgtgccg acaacgaggc cgaggccgct tcggcgccct ccgacgcagc
     1981 cgcgcaggcg cgcaccgagg cgtccgaacc gacgatcgac tcgcgtccgg cccagtcgaa
     2041 caacggcaac aatgatcggc agcagcagtc gcgcgacgac gacgatttcg gcaccggacg
     2101 ccgctcgcgt cgccgccgca accgcgaccg ccagagcaac aacaacaacc gccggggcaa
     2161 caacaaccgg ggccagtctg cgtcgatggc cggcctggac gccgagccgg tggtcaacga
     2221 cgacgacgtg ctgctgaacg tcagcggcat tctggacgtg ctcgacaact acgcgttcgt
     2281 gcgcacctcg ggctatctgc cgggcaaca
//
LOCUS       DAOSUG010000495         2308 bp    DNA     linear   ENV 10-JUN-2024
DEFINITION  MAG TPA_asm: Micropruina sp. isolate AMR_MDS_2195
            NODE_18829_length_2308_cov_4.677319, whole genome shotgun sequence.
ACCESSION   DAOSUG010000495 DAOSUG010000000
VERSION     DAOSUG010000495.1
DBLINK      BioProject: PRJNA925477
            BioSample: SAMN32864262
            Sequence Read Archive: SRR4236673
KEYWORDS    WGS; ENV; Metagenome Assembled Genome; MAG; Third Party Data; TPA;
            TPA:assembly.
SOURCE      Micropruina sp. (wastewater metagenome)
  ORGANISM  Micropruina sp.
            Bacteria; Bacillati; Actinomycetota; Actinomycetes;
            Propionibacteriales; Nocardioidaceae; Micropruina.
REFERENCE   1  (bases 1 to 2308)
  AUTHORS   Abdulkadir,N., Saraiva,J.P., Zhang,J., Stolte,S., Gillor,O.,
            Harms,H. and Rocha,U.
  TITLE     Genome-centric analyses of 165 metagenomes show that mobile genetic
            elements are crucial for the transmission of antimicrobial
            resistance genes to pathogens in activated sludge and wastewater
  JOURNAL   Microbiol Spectr 12 (3), e0291823 (2024)
   PUBMED   38289113
REFERENCE   2  (bases 1 to 2308)
  AUTHORS   Abdulkadir,N.
  TITLE     Direct Submission
  JOURNAL   Submitted (24-MAR-2023) Environmental Microbiology, Helmholtz
            Center for Environmental Research -UFZ, Permoserstr 15, Leipzig
            04318, Germany
COMMENT     The annotation was added by the NCBI Prokaryotic Genome Annotation
            Pipeline (PGAP). Information about PGAP can be found here:
            https://www.ncbi.nlm.nih.gov/genome/annotation_prok/
            
            ##Genome-Assembly-Data-START##
            Assembly Method        :: SPAdes v. 3.11.1
            Genome Representation  :: Full
            Expected Final Version :: Yes
            Genome Coverage        :: Not Applicable
            Sequencing Technology  :: Illumina MiSeq
            ##Genome-Assembly-Data-END##
            
            ##Genome-Annotation-Data-START##
            Annotation Provider               :: NCBI
            Annotation Date                   :: 05/04/2023 04:57:10
            Annotation Pipeline               :: NCBI Prokaryotic Genome
                                                 Annotation Pipeline (PGAP)
            Annotation Method                 :: Best-placed reference protein
                                                 set; GeneMarkS-2+
            Annotation Software revision      :: 6.5
            Features Annotated                :: Gene; CDS; rRNA; tRNA; ncRNA
            Genes (total)                     :: 2,776
            CDSs (total)                      :: 2,750
            Genes (coding)                    :: 2,714
            CDSs (with protein)               :: 2,714
            Genes (RNA)                       :: 26
            tRNAs                             :: 24
            ncRNAs                            :: 2
            Pseudo Genes (total)              :: 36
            CDSs (without protein)            :: 36
            Pseudo Genes (ambiguous residues) :: 0 of 36
            Pseudo Genes (frameshifted)       :: 2 of 36
            Pseudo Genes (incomplete)         :: 32 of 36
            Pseudo Genes (internal stop)      :: 2 of 36
            ##Genome-Annotation-Data-END##
FEATURES             Location/Qualifiers
     source          1..2308
                     /organism="Micropruina sp."
                     /mol_type="genomic DNA"
                     /submitter_seqid="NODE_18829_length_2308_cov_4.677319"
                     /isolate="AMR_MDS_2195"
                     /isolation_source="Activated sludge of municipal
                     wastewater treatment plants"
                     /db_xref="taxon:2737536"
                     /environmental_sample
                     /geo_loc_name="Canada"
                     /lat_lon="49.25 N 123.1 W"
                     /collection_date="2015-06-16"
                     /metagenome_source="wastewater metagenome"
                     /note="metagenomic"
     gene            complement(<1..362)
                     /locus_tag="PLF56_13210"
     CDS             complement(<1..362)
                     /locus_tag="PLF56_13210"
                     /inference="COORDINATES: ab initio
                     prediction:GeneMarkS-2+"
                     /note="Derived by automated computational analysis using
                     gene prediction method: GeneMarkS-2+."
                     /codon_start=1
                     /transl_table=11
                     /product="hypothetical protein"
                     /protein_id="HOQ54556.1"
                     /translation="MTLDHPTNASARLGHRRLVALTFSLLGLHVGVRAVQLADQAIAV
                     DLDAGQLGTGASLSAAAGIITLIGGALLVDRLGRRPILALGLIGTGASFWAQAVVTNY
                     PQFLAASLGYGLLVSFLDL"
     gene            665..2065
                     /locus_tag="PLF56_13215"
     CDS             665..2065
                     /locus_tag="PLF56_13215"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_009884204.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_function: GO:0004803 - transposase activity [Evidence
                     IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="IS1380 family transposase"
                     /protein_id="HOQ54557.1"
                     /translation="MNKRSGMYPRLRVDSAAVPAVAQAGGVLLTRTVETSGLGILLSR
                     ALGPWRKSLARHDPAKVLLDLAISLALGGDACRDAALLRSEPGVYGRVASDATISRTI
                     TALAADVDRVEKAVAQATAAARQHVWGLAGEAAPGHGASAGDPIIIDLDATLVTSHSD
                     KELARPTFKKGYGFHPLLAFVDHGPDGTGEPMAGLLRSGNAGSNTAADHETVIKAALK
                     QVPGINPARPGKKVLIRTDGAGGSKQLLGFLARRGLSYSIGFTLPMSTPELYHLVPEH
                     AWQDAYNADGQVRDGAAVVEFTDLLTAKDLLKGYPTGMRVIVRRERPHPGAQLRFDDV
                     DGYRLTAFVTNTARGQLADLEMRHRRRARCEDRIRTAKDTGLTNLPLQGYDQNRIWLL
                     IVQLASDLLAWMAMLALQGPARRWEPKTLRHRLFTIAATLARTGRRTILHLKQASPWS
                     PMILDGWNRLSALTPP"
BASE COUNT          426 a          759 c          763 g          360 t
ORIGIN      
        1 aggtccagga agctcacgag caggccgtag ccgaggctgg cggccaggaa ttgcgggtaa
       61 ttggtgacaa cggcctgggc ccagaagctg gcgccggtgc cgatcagtcc gagcgcgagg
      121 atggggcgtc ggcccagacg gtcgacgagc aaggctccgc cgatgagggt gatgatgcct
      181 gcggccgcgc tgaggcttgc gcccgtgccg agttgtccgg cgtcgaggtc aacggcgatg
      241 gcttgatcgg cgagctggac ggctcggacg ccgacgtgga ggccgagcag gctgaaggtg
      301 agcgcgacga ggcgacggtg gccaaggcgc gcggatgcgt tggtggggtg gtcgagtgtc
      361 atgggtggtc ctgatgtttg agtggtcggg tggagcgccc gcactcgtgc atgaccacca
      421 gccgtggatg ttcgtcgtcc acggttcaca agaaaggggg gtcaggggtg ctggacgcga
      481 acgaaccgcg tccggcctat ctcaccctcg tcgtcgatga tgtccagaac cctcatgatt
      541 cgacggacgt tagcccggag atttcacggg ggtggctgga cgtgaaggag ggaagtgccc
      601 gctgacctgc gaagataccg attgtctagg tctgtatcag cgtggttgaa ggagcacttc
      661 ccaggtgaac aagcgtagcg ggatgtatcc gcgtctccga gtcgactccg ctgcggtgcc
      721 ggcggtggcc caggcgggcg gggtgctgct cacccggacc gtggagacct ccggcctggg
      781 aatcttgttg agccgggcgc tgggaccgtg gcgcaaatcc ctcgcgaggc atgacccggc
      841 gaaggtgctg ctggatctgg cgatcagcct cgcgctgggt ggtgatgcct gtcgtgacgc
      901 cgcgctgctg cgcagcgaac ccggtgtcta tggccgggtg gcctcggatg cgacgatctc
      961 gcgcaccatc accgccctcg cggccgatgt cgaccgggtg gagaaagccg tggcccaggc
     1021 cactgcagca gctcgccagc acgtctgggg cctggcaggc gaggcggcac caggccatgg
     1081 ggcgagcgcc ggggatccga tcatcattga tctggacgcc actctcgtca cgtcacattc
     1141 ggacaaggaa ctggcccggc cgacgttcaa gaagggctac ggtttccacc cgctgttggc
     1201 cttcgtcgac cacggccccg acgggaccgg cgaacccatg gcagggctgc tcaggtcggg
     1261 caatgccggc tccaacaccg ccgccgatca cgagaccgtc atcaaggctg cgctcaaaca
     1321 ggtgcccggg atcaaccccg ccaggccggg caagaaggtg ctgatccgta ctgacggggc
     1381 cggcggatcc aaacagctac tcggcttcct cgcccggcgc ggactgtcgt actcgatcgg
     1441 cttcacgctg ccgatgagca ccccggagtt gtatcacctt gttcccgagc acgcgtggca
     1501 ggatgcctac aacgccgacg gccaggtccg cgacggcgca gccgtggtcg agttcaccga
     1561 cctcctcacc gccaaagacc tgttgaaggg ctaccccacc gggatgcgag tgatcgtgcg
     1621 ccgagaacga ccccaccccg gcgcccaact ccgctttgat gacgtcgatg gctaccggct
     1681 caccgccttc gtcaccaaca ctgccagagg ccagttggct gatttggaga tgcggcaccg
     1741 ccgccgcgcc cgttgtgagg accgcatccg caccgcaaaa gacaccggcc tgaccaacct
     1801 gccgttgcag ggctatgacc agaatcgcat ctggctgctc atcgtccagc tggccagcga
     1861 cctgctcgcc tggatggcca tgctcgccct ccaaggccct gcgcggcgat gggaacccaa
     1921 aaccctgcgg caccgcctgt tcacgatcgc cgccaccttg gcgcgcacag gcaggcgcac
     1981 catcctgcac ctcaaacagg ccagcccctg gtcacccatg attctggacg gctggaacag
     2041 gctctccgca ctcaccccac cctgacccaa ccgtcacccg ccccgacgac cagcagtagc
     2101 cattcggcag tggaaccgac gcccacccgc aacgacatgc gggcacttgt cacacccaca
     2161 tgccaaaatc acgagcatga gaccggcaac gacgctggac cgttgaccaa ccagccctca
     2221 tgaaagatcg aggctagaga cccatgcgtc caagacgagg taacccgacc aagcctcgaa
     2281 tgctgtcagg tgagacaggg caggcggc
//
LOCUS       DAOSUG010000496         2285 bp    DNA     linear   ENV 10-JUN-2024
DEFINITION  MAG TPA_asm: Micropruina sp. isolate AMR_MDS_2195
            NODE_19097_length_2285_cov_4.204933, whole genome shotgun sequence.
ACCESSION   DAOSUG010000496 DAOSUG010000000
VERSION     DAOSUG010000496.1
DBLINK      BioProject: PRJNA925477
            BioSample: SAMN32864262
            Sequence Read Archive: SRR4236673
KEYWORDS    WGS; ENV; Metagenome Assembled Genome; MAG; Third Party Data; TPA;
            TPA:assembly.
SOURCE      Micropruina sp. (wastewater metagenome)
  ORGANISM  Micropruina sp.
            Bacteria; Bacillati; Actinomycetota; Actinomycetes;
            Propionibacteriales; Nocardioidaceae; Micropruina.
REFERENCE   1  (bases 1 to 2285)
  AUTHORS   Abdulkadir,N., Saraiva,J.P., Zhang,J., Stolte,S., Gillor,O.,
            Harms,H. and Rocha,U.
  TITLE     Genome-centric analyses of 165 metagenomes show that mobile genetic
            elements are crucial for the transmission of antimicrobial
            resistance genes to pathogens in activated sludge and wastewater
  JOURNAL   Microbiol Spectr 12 (3), e0291823 (2024)
   PUBMED   38289113
REFERENCE   2  (bases 1 to 2285)
  AUTHORS   Abdulkadir,N.
  TITLE     Direct Submission
  JOURNAL   Submitted (24-MAR-2023) Environmental Microbiology, Helmholtz
            Center for Environmental Research -UFZ, Permoserstr 15, Leipzig
            04318, Germany
COMMENT     The annotation was added by the NCBI Prokaryotic Genome Annotation
            Pipeline (PGAP). Information about PGAP can be found here:
            https://www.ncbi.nlm.nih.gov/genome/annotation_prok/
            
            ##Genome-Assembly-Data-START##
            Assembly Method        :: SPAdes v. 3.11.1
            Genome Representation  :: Full
            Expected Final Version :: Yes
            Genome Coverage        :: Not Applicable
            Sequencing Technology  :: Illumina MiSeq
            ##Genome-Assembly-Data-END##
            
            ##Genome-Annotation-Data-START##
            Annotation Provider               :: NCBI
            Annotation Date                   :: 05/04/2023 04:57:10
            Annotation Pipeline               :: NCBI Prokaryotic Genome
                                                 Annotation Pipeline (PGAP)
            Annotation Method                 :: Best-placed reference protein
                                                 set; GeneMarkS-2+
            Annotation Software revision      :: 6.5
            Features Annotated                :: Gene; CDS; rRNA; tRNA; ncRNA
            Genes (total)                     :: 2,776
            CDSs (total)                      :: 2,750
            Genes (coding)                    :: 2,714
            CDSs (with protein)               :: 2,714
            Genes (RNA)                       :: 26
            tRNAs                             :: 24
            ncRNAs                            :: 2
            Pseudo Genes (total)              :: 36
            CDSs (without protein)            :: 36
            Pseudo Genes (ambiguous residues) :: 0 of 36
            Pseudo Genes (frameshifted)       :: 2 of 36
            Pseudo Genes (incomplete)         :: 32 of 36
            Pseudo Genes (internal stop)      :: 2 of 36
            ##Genome-Annotation-Data-END##
FEATURES             Location/Qualifiers
     source          1..2285
                     /organism="Micropruina sp."
                     /mol_type="genomic DNA"
                     /submitter_seqid="NODE_19097_length_2285_cov_4.204933"
                     /isolate="AMR_MDS_2195"
                     /isolation_source="Activated sludge of municipal
                     wastewater treatment plants"
                     /db_xref="taxon:2737536"
                     /environmental_sample
                     /geo_loc_name="Canada"
                     /lat_lon="49.25 N 123.1 W"
                     /collection_date="2015-06-16"
                     /metagenome_source="wastewater metagenome"
                     /note="metagenomic"
     gene            complement(<1..222)
                     /locus_tag="PLF56_13220"
     CDS             complement(<1..222)
                     /locus_tag="PLF56_13220"
                     /inference="COORDINATES: ab initio
                     prediction:GeneMarkS-2+"
                     /note="Derived by automated computational analysis using
                     gene prediction method: GeneMarkS-2+."
                     /codon_start=1
                     /transl_table=11
                     /product="hypothetical protein"
                     /protein_id="HOQ54558.1"
                     /translation="MAIEQPFVHDLTVALAAPVQVWSAPGGDIDGSGVQGAYYGDHRM
                     VRGLQLDLQGPDGPIELVHGATSVGADGTV"
     gene            442..570
                     /locus_tag="PLF56_13225"
     CDS             442..570
                     /locus_tag="PLF56_13225"
                     /inference="COORDINATES: ab initio
                     prediction:GeneMarkS-2+"
                     /note="Derived by automated computational analysis using
                     gene prediction method: GeneMarkS-2+."
                     /codon_start=1
                     /transl_table=11
                     /product="hypothetical protein"
                     /protein_id="HOQ54559.1"
                     /translation="MSSGNQYPIKLAAATLMTIPVAVIFFIFQRRIMNTTGGAVKD"
     gene            861..2171
                     /locus_tag="PLF56_13230"
     CDS             861..2171
                     /locus_tag="PLF56_13230"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_020388515.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="extracellular solute-binding protein"
                     /protein_id="HOQ54560.1"
                     /translation="MRTTFIRTAVVPTALLALLATGCSQPAAAPSPTAASTTAASAEP
                     VTLTVATFNEFGYEDLFKEYTKLHPNVTITPKKAATSNEARENYFNKLAAGSGLSDIE
                     AIEVDWLPDVMQTADKLADLGGDDVKGRWLDWKSAAATTPDGKLVGFGTDIGPEAVCY
                     RSDLFKKAGLPTDREEVAKLLQGDWNKYFEVGKQYVAKSGGVAWYDSADAIMQGQINQ
                     VANAFSSTQPEETLIPLDQNQPVKDMYNSILKASVDDKLSANLQQWSTDWVNAFQKGK
                     FATMLCPGWMLGVVEGNAKGVEGWDIANVFPGGGGNWGGSYLTVPAQGKNTAAARELA
                     AWLTAPEQQIKAFKAKGTFPSQVEALKSPELLGVTNTFFNNAPTGQILADRANAVTVN
                     PFKGPQYFAVRQVIADAINRVDVTKKQDAATSWAQAVKEFNALG"
BASE COUNT          418 a          808 c          715 g          344 t
ORIGIN      
        1 caccgttccg tcggcgccta ccgaggttgc accgtggacc agttcgatcg ggccgtccgg
       61 gccctgcagg tcgagctgca gccccctgac catgcggtga tcgccgtagt aggcgccctg
      121 gacgccgctg ccgtcgatgt ctccaccggg cgccgaccac acctgcaccg gcgcggccag
      181 cgccaccgtg aggtcgtgca cgaacggctg ctcgatggcc atcacacacc tccggttcga
      241 cggtccagca atgcctcggt tcaatacctg ccaaggggtt gacatcgggt tgtggcactc
      301 cgaagattag ggcggggtag agccggtagg ccacgttcat cgaacgccgg gcacgcaacg
      361 cccgtccggc cgcagctttc tgggctggcc cgagggatcc ggcgtgggcg acgaccgctg
      421 tggtcgctgg cctcgggcca gttgtcgtcg ggcaaccagt acccgatcaa gttggcggcg
      481 gccacgctga tgaccatccc ggttgcggtg atcttcttca tcttccagcg tcgcatcatg
      541 aataccaccg gaggcgcggt caaggactga gccaggtgcg tgcccaacgc ggggtagtcg
      601 gtgtggaagc cagcccccgc ggagggccac gggccagagg tcggtctgca tctccacgcc
      661 accgcccacc ccgggcgtcc acccgcgccc gctgatccga gtgtcactaa caatcacaat
      721 ctgatctcgg tcgtccaaaa ccttgccgtg tgaaagcgct ctcaggcatg atctagctca
      781 ggatgagagc gctctcatcc agggaggaca tctcccgccg cgcgggccac ctcggtccgc
      841 tcacgcgacg aaggagtctc atgcgcacga cattcatccg cacggcggtg gttcccactg
      901 ctctgctggc actcttggcc acgggttgct cgcagcccgc ggcagccccc tcgcccaccg
      961 cggcgagcac cacggcagcg tccgccgaac cggtcaccct gaccgtcgcc acgttcaacg
     1021 agttcgggta cgaagacctg ttcaaggagt acaccaagct gcacccgaac gtcaccatca
     1081 ccccgaagaa ggccgccacc tcgaacgagg cgcgcgagaa ctacttcaac aagctggccg
     1141 caggctcggg cctgtccgac atcgaggcga tcgaggtcga ctggttgccc gacgtgatgc
     1201 agaccgcaga caagctcgcc gacctcggcg gtgacgacgt caagggtcgc tggctcgact
     1261 ggaagtcggc cgccgccacc acaccggacg gcaagctggt cggtttcggt accgacatcg
     1321 gcccggaggc ggtgtgctac cgctccgacc tgttcaagaa ggccggcctg cccaccgatc
     1381 gtgaagaggt cgccaaactg ctgcagggcg actggaacaa gtacttcgag gtcggcaagc
     1441 agtacgtggc caagtccggc ggcgtggcct ggtacgactc ggccgacgcg atcatgcagg
     1501 gccagatcaa ccaggtcgcg aacgccttct cgtccaccca gcccgaagag acgctgattc
     1561 cgctcgacca gaaccagccg gtcaaagaca tgtacaactc gatcctcaag gcctccgtcg
     1621 acgacaagct gtcggccaac ctgcagcagt ggagcaccga ctgggtcaac gccttccaga
     1681 aaggcaagtt cgccacgatg ctctgcccgg gttggatgct cggcgtcgtc gagggcaacg
     1741 ccaagggcgt cgagggctgg gacatcgcca acgtcttccc cggtggtggc ggcaactggg
     1801 gcggctcgta cctgaccgtt cccgcgcagg gcaagaacac cgcagccgcc cgcgagttgg
     1861 ccgcctggct gactgctccc gagcagcaga tcaaggcgtt caaggccaag ggcacgttcc
     1921 cgagccaggt cgaggcgctg aagagccctg agttgctcgg cgtgaccaac acgttcttca
     1981 acaacgcgcc gaccggccag atccttgccg accgggccaa cgcggtcacc gtcaacccct
     2041 tcaagggccc gcagtacttc gccgtccggc aggtgatcgc cgatgcgatc aaccgcgtcg
     2101 acgtgaccaa gaagcaggac gccgcgacgt cctgggccca ggcggtcaag gagttcaacg
     2161 ccctcggctg acagccgccg acgctgaacc cccgggtcct ggacggggcg gtaccaccgc
     2221 tccgtccagg ctcccgccgg caccacgcag tcgccggaca cccgcgcttg ccgcacaccg
     2281 gccgc
//
LOCUS       DAOSUG010000497         2282 bp    DNA     linear   ENV 10-JUN-2024
DEFINITION  MAG TPA_asm: Micropruina sp. isolate AMR_MDS_2195
            NODE_19124_length_2282_cov_5.092501, whole genome shotgun sequence.
ACCESSION   DAOSUG010000497 DAOSUG010000000
VERSION     DAOSUG010000497.1
DBLINK      BioProject: PRJNA925477
            BioSample: SAMN32864262
            Sequence Read Archive: SRR4236673
KEYWORDS    WGS; ENV; Metagenome Assembled Genome; MAG; Third Party Data; TPA;
            TPA:assembly.
SOURCE      Micropruina sp. (wastewater metagenome)
  ORGANISM  Micropruina sp.
            Bacteria; Bacillati; Actinomycetota; Actinomycetes;
            Propionibacteriales; Nocardioidaceae; Micropruina.
REFERENCE   1  (bases 1 to 2282)
  AUTHORS   Abdulkadir,N., Saraiva,J.P., Zhang,J., Stolte,S., Gillor,O.,
            Harms,H. and Rocha,U.
  TITLE     Genome-centric analyses of 165 metagenomes show that mobile genetic
            elements are crucial for the transmission of antimicrobial
            resistance genes to pathogens in activated sludge and wastewater
  JOURNAL   Microbiol Spectr 12 (3), e0291823 (2024)
   PUBMED   38289113
REFERENCE   2  (bases 1 to 2282)
  AUTHORS   Abdulkadir,N.
  TITLE     Direct Submission
  JOURNAL   Submitted (24-MAR-2023) Environmental Microbiology, Helmholtz
            Center for Environmental Research -UFZ, Permoserstr 15, Leipzig
            04318, Germany
COMMENT     The annotation was added by the NCBI Prokaryotic Genome Annotation
            Pipeline (PGAP). Information about PGAP can be found here:
            https://www.ncbi.nlm.nih.gov/genome/annotation_prok/
            
            ##Genome-Assembly-Data-START##
            Assembly Method        :: SPAdes v. 3.11.1
            Genome Representation  :: Full
            Expected Final Version :: Yes
            Genome Coverage        :: Not Applicable
            Sequencing Technology  :: Illumina MiSeq
            ##Genome-Assembly-Data-END##
            
            ##Genome-Annotation-Data-START##
            Annotation Provider               :: NCBI
            Annotation Date                   :: 05/04/2023 04:57:10
            Annotation Pipeline               :: NCBI Prokaryotic Genome
                                                 Annotation Pipeline (PGAP)
            Annotation Method                 :: Best-placed reference protein
                                                 set; GeneMarkS-2+
            Annotation Software revision      :: 6.5
            Features Annotated                :: Gene; CDS; rRNA; tRNA; ncRNA
            Genes (total)                     :: 2,776
            CDSs (total)                      :: 2,750
            Genes (coding)                    :: 2,714
            CDSs (with protein)               :: 2,714
            Genes (RNA)                       :: 26
            tRNAs                             :: 24
            ncRNAs                            :: 2
            Pseudo Genes (total)              :: 36
            CDSs (without protein)            :: 36
            Pseudo Genes (ambiguous residues) :: 0 of 36
            Pseudo Genes (frameshifted)       :: 2 of 36
            Pseudo Genes (incomplete)         :: 32 of 36
            Pseudo Genes (internal stop)      :: 2 of 36
            ##Genome-Annotation-Data-END##
FEATURES             Location/Qualifiers
     source          1..2282
                     /organism="Micropruina sp."
                     /mol_type="genomic DNA"
                     /submitter_seqid="NODE_19124_length_2282_cov_5.092501"
                     /isolate="AMR_MDS_2195"
                     /isolation_source="Activated sludge of municipal
                     wastewater treatment plants"
                     /db_xref="taxon:2737536"
                     /environmental_sample
                     /geo_loc_name="Canada"
                     /lat_lon="49.25 N 123.1 W"
                     /collection_date="2015-06-16"
                     /metagenome_source="wastewater metagenome"
                     /note="metagenomic"
     gene            <1..311
                     /locus_tag="PLF56_13235"
     CDS             <1..311
                     /locus_tag="PLF56_13235"
                     /inference="COORDINATES: ab initio
                     prediction:GeneMarkS-2+"
                     /note="Derived by automated computational analysis using
                     gene prediction method: GeneMarkS-2+."
                     /codon_start=3
                     /transl_table=11
                     /product="hypothetical protein"
                     /protein_id="HOQ54561.1"
                     /translation="EGLPVEVVSSDEPGVVSLTSWANDGSIVTLTWDETAGSVHVRWT
                     EADEERLVLERETVSKVSVREDHGRIEFWIWSDAGGVVGQLLVRVGDHVGVSDALLRR
                     "
     gene            510..662
                     /locus_tag="PLF56_13240"
     CDS             510..662
                     /locus_tag="PLF56_13240"
                     /inference="COORDINATES: ab initio
                     prediction:GeneMarkS-2+"
                     /note="Derived by automated computational analysis using
                     gene prediction method: GeneMarkS-2+."
                     /codon_start=1
                     /transl_table=11
                     /product="hypothetical protein"
                     /protein_id="HOQ54562.1"
                     /translation="MSTQVEPVVVEAAELEEQQVAMVEQPPLVVDKQLSWLGPSPKPE
                     YPTLTS"
     gene            1307..1711
                     /locus_tag="PLF56_13245"
     CDS             1307..1711
                     /locus_tag="PLF56_13245"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_020390550.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_function: GO:0003677 - DNA binding [Evidence IEA];
                     GO_function: GO:0003700 - DNA-binding transcription factor
                     activity [Evidence IEA];
                     GO_process: GO:0006355 - regulation of DNA-templated
                     transcription [Evidence IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="metalloregulator ArsR/SmtB family transcription
                     factor"
                     /protein_id="HOQ54563.1"
                     /translation="MTIQGLDGCAVPQVHPDKVRLVTINAPDADELARVSAVFKLLGD
                     PTRTRLLYALLEAGELCVCDLAAATSTAEATVSQALRLLRASSVVTGRREGRNVFYRL
                     SDAHVRLLLDVTRAHITHGGPETLRDGDGSLR"
     gene            1708..>2282
                     /locus_tag="PLF56_13250"
     CDS             1708..>2282
                     /locus_tag="PLF56_13250"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_008359795.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_function: GO:0008324 - monoatomic cation transmembrane
                     transporter activity [Evidence IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="cation diffusion facilitator family transporter"
                     /protein_id="HOQ54564.1"
                     /translation="MSHSHSHDHAGATGRHRWRLAVAFVLVAGFFVVELLAAWSSGSL
                     ALLSDAGHMAADVTTLGAALIATRLATRKDTTGRRTFGSYRAEVFASGLAVLLMIGVG
                     IYIAIEAVGRIGAAPELASGLMLVVGVLGLIVNAICLMLLRAGAAESLNVKGAYLEVI
                     ADLLGSIGVIVAAVLVRFTGSAWWDTVIALA"
BASE COUNT          367 a          668 c          779 g          468 t
ORIGIN      
        1 tcgaaggcct tcccgtcgag gtggtgagct cggacgagcc gggggtcgtc agcttgacca
       61 gttgggctaa cgacggctct attgtcacgc tcacgtggga tgagaccgca ggatcggtcc
      121 acgtacgctg gactgaggcc gacgaagagc gattggtcct cgagcgagag acggtgtcca
      181 aggtgtccgt gcgcgaggac cacggtcgga tcgagttctg gatctggtcg gacgccggtg
      241 gcgtagttgg gcagttgctc gtccgtgtcg gtgatcatgt cggcgtttcg gacgcactgc
      301 tgcgcagata gccagatcag ctgccgctgg cgaggacctc cagcctggtt tggtgacgag
      361 ggcgggatct tcgctgttca gctctgagac cgccggccgt ttggcttgct cgcgccgcga
      421 gatcagtggg aactactgat ctacttttgg tgctaccgag agtcgggact gtactttcaa
      481 cggtgtaact ccgatcagtg aggtacatca tgagcacgca ggttgagccg gtcgtcgtcg
      541 aggcggccga gttggaggag cagcaggtgg cgatggtcga gcagccgccg ttggtggtgg
      601 acaagcagct tagctggttg ggcccctccc caaagccaga atatccaact ctgactagct
      661 agagactcag cgttaacgat ctgggcgcgg aaggaactga gatatggaga aaaattcgca
      721 aggatcctcg atactgtcaa gattcgttaa gagggttgcc ggcttgtgtg tttcgctctc
      781 ctcacagcga cacttcttca tctggcgaca aactggatgg gtgccggtct ttgggattct
      841 tatgataatg ccgatgctga tcgtggcggt tatcccgtcg tggattgggc cttgggtctt
      901 ctatacagtc ggtttcttcg ctctggcccc ctttttggat ggattgatag tatacctagt
      961 cgatcgaccc cgccgtatgg ataggcaatg agtggtacat tttgccaact tccgggcgtc
     1021 gattccacga agtgcgacgt cattgcgcag gatcgcactg gcgcggagtg cagagtgcag
     1081 aggaagtgag gttgacccca taacccgtgt ccaggttacg gggccaacct cagagtgctg
     1141 tcgtgggcga tcctttcgcg acgaggaacg agctgccaaa cacacttccg cccatgtccg
     1201 tcagctcggt tgccatctcc ccgactagag tctctcggcc cgacgccagg gtcgagacgt
     1261 gtccgggcta tcatctgatc atctcgtcag atgaaaggag atcatcgtga caatccaggg
     1321 cctggatggg tgcgcggtgc cgcaggtgca tcccgacaag gtgcggttgg tcacgatcaa
     1381 cgctcccgat gcggacgaac tggccagggt cagcgcggtg ttcaagttgc tgggcgatcc
     1441 gacgcgcacc cgactgctgt acgcactgct cgaggccggc gaactgtgtg tgtgcgacct
     1501 cgccgccgcc acctcgaccg ccgaggcgac cgtgtcgcag gcgctgcggc tgctgcgcgc
     1561 ctcgtccgtg gtcaccggac ggcgcgaggg ccgcaacgtg ttctaccgcc tctcggacgc
     1621 gcacgtgcgg ctgctgctcg acgtgacccg cgctcacatc acccatggcg gcccggagac
     1681 cctgcgcgac ggggacggga gcctccggtg agtcattcgc attcgcacga tcacgcgggc
     1741 gcgaccgggc gtcaccgctg gcggctggcg gtcgccttcg tgctggtggc cggcttcttc
     1801 gtggtcgagt tgctggcggc gtggagctcg ggttcgctgg ccctgctgtc ggacgccgga
     1861 cacatggccg ccgacgtgac cacgctgggc gccgccctga tcgccacccg gctggccact
     1921 cgcaaggaca ccaccggacg gcgcaccttc ggctcgtacc gggccgaggt gttcgcctcg
     1981 gggctggcgg tgctgctgat gatcggcgtc ggcatctaca tcgcgatcga agccgtcgga
     2041 cggatcgggg ccgcgcccga gctggcgtcc gggctgatgc tggtggtcgg cgtgctgggt
     2101 ctgatcgtga acgcgatctg tctgatgctg ttgcgcgccg gggcggccga gagcctgaac
     2161 gtgaagggcg cctacctcga ggtgatcgcc gacctgctcg gctcgatcgg tgtgatcgtg
     2221 gcggccgtgc tggtccggtt caccggcagc gcctggtggg acaccgtgat cgcccttgcc
     2281 at
//
LOCUS       DAOSUG010000498         2264 bp    DNA     linear   ENV 10-JUN-2024
DEFINITION  MAG TPA_asm: Micropruina sp. isolate AMR_MDS_2195
            NODE_19320_length_2264_cov_4.126301, whole genome shotgun sequence.
ACCESSION   DAOSUG010000498 DAOSUG010000000
VERSION     DAOSUG010000498.1
DBLINK      BioProject: PRJNA925477
            BioSample: SAMN32864262
            Sequence Read Archive: SRR4236673
KEYWORDS    WGS; ENV; Metagenome Assembled Genome; MAG; Third Party Data; TPA;
            TPA:assembly.
SOURCE      Micropruina sp. (wastewater metagenome)
  ORGANISM  Micropruina sp.
            Bacteria; Bacillati; Actinomycetota; Actinomycetes;
            Propionibacteriales; Nocardioidaceae; Micropruina.
REFERENCE   1  (bases 1 to 2264)
  AUTHORS   Abdulkadir,N., Saraiva,J.P., Zhang,J., Stolte,S., Gillor,O.,
            Harms,H. and Rocha,U.
  TITLE     Genome-centric analyses of 165 metagenomes show that mobile genetic
            elements are crucial for the transmission of antimicrobial
            resistance genes to pathogens in activated sludge and wastewater
  JOURNAL   Microbiol Spectr 12 (3), e0291823 (2024)
   PUBMED   38289113
REFERENCE   2  (bases 1 to 2264)
  AUTHORS   Abdulkadir,N.
  TITLE     Direct Submission
  JOURNAL   Submitted (24-MAR-2023) Environmental Microbiology, Helmholtz
            Center for Environmental Research -UFZ, Permoserstr 15, Leipzig
            04318, Germany
COMMENT     The annotation was added by the NCBI Prokaryotic Genome Annotation
            Pipeline (PGAP). Information about PGAP can be found here:
            https://www.ncbi.nlm.nih.gov/genome/annotation_prok/
            
            ##Genome-Assembly-Data-START##
            Assembly Method        :: SPAdes v. 3.11.1
            Genome Representation  :: Full
            Expected Final Version :: Yes
            Genome Coverage        :: Not Applicable
            Sequencing Technology  :: Illumina MiSeq
            ##Genome-Assembly-Data-END##
            
            ##Genome-Annotation-Data-START##
            Annotation Provider               :: NCBI
            Annotation Date                   :: 05/04/2023 04:57:10
            Annotation Pipeline               :: NCBI Prokaryotic Genome
                                                 Annotation Pipeline (PGAP)
            Annotation Method                 :: Best-placed reference protein
                                                 set; GeneMarkS-2+
            Annotation Software revision      :: 6.5
            Features Annotated                :: Gene; CDS; rRNA; tRNA; ncRNA
            Genes (total)                     :: 2,776
            CDSs (total)                      :: 2,750
            Genes (coding)                    :: 2,714
            CDSs (with protein)               :: 2,714
            Genes (RNA)                       :: 26
            tRNAs                             :: 24
            ncRNAs                            :: 2
            Pseudo Genes (total)              :: 36
            CDSs (without protein)            :: 36
            Pseudo Genes (ambiguous residues) :: 0 of 36
            Pseudo Genes (frameshifted)       :: 2 of 36
            Pseudo Genes (incomplete)         :: 32 of 36
            Pseudo Genes (internal stop)      :: 2 of 36
            ##Genome-Annotation-Data-END##
FEATURES             Location/Qualifiers
     source          1..2264
                     /organism="Micropruina sp."
                     /mol_type="genomic DNA"
                     /submitter_seqid="NODE_19320_length_2264_cov_4.126301"
                     /isolate="AMR_MDS_2195"
                     /isolation_source="Activated sludge of municipal
                     wastewater treatment plants"
                     /db_xref="taxon:2737536"
                     /environmental_sample
                     /geo_loc_name="Canada"
                     /lat_lon="49.25 N 123.1 W"
                     /collection_date="2015-06-16"
                     /metagenome_source="wastewater metagenome"
                     /note="metagenomic"
     gene            complement(<1..423)
                     /locus_tag="PLF56_13255"
     CDS             complement(<1..423)
                     /locus_tag="PLF56_13255"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_002538283.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="pyridoxamine 5'-phosphate oxidase family
                     protein"
                     /protein_id="HOQ54565.1"
                     /translation="MANRPPITRLDEAEAWQLLGSAQVGRLATVIERQPDIFPVNFVL
                     DGPAVVFRTAEGSKLLQLIVNSGVAFEVDSWTESTGWSVVVKGHAQELTEWDDLARVE
                     QLPLRPWVPTLKTHFIRIAVDQIEGRRFVFGDEPEREVD"
     gene            474..1052
                     /locus_tag="PLF56_13260"
     CDS             474..1052
                     /locus_tag="PLF56_13260"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_011756720.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="RsmD family RNA methyltransferase"
                     /protein_id="HOQ54566.1"
                     /translation="MSRIIAGSRKGQRLLTPAGDATRPTSDRVREAAFGALASELGRA
                     GDPASMLEGCGFLDLFAGSGAVALEAASRGAAPVVAVEKERRAVEVIRRNVQQTGLGV
                     RVVSAGVGAFLGGEPTPFRLCWLDPPYELGNERIEPLVSRLVQGWLAPGGIVVVERAR
                     RSGAFEWPEQLGDRWHRRYGETTLFFATEGEA"
     gene            1049..1531
                     /gene="coaD"
                     /locus_tag="PLF56_13265"
     CDS             1049..1531
                     /gene="coaD"
                     /locus_tag="PLF56_13265"
                     /EC_number="2.7.7.3"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_014846341.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_function: GO:0004595 - pantetheine-phosphate
                     adenylyltransferase activity [Evidence IEA];
                     GO_process: GO:0015937 - coenzyme A biosynthetic process
                     [Evidence IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="pantetheine-phosphate adenylyltransferase"
                     /protein_id="HOQ54567.1"
                     /translation="MRAVCPGSYDPITHGHLNIIERAAALFGDVIVAVGRNSAKSYLF
                     DTDERVELARAVTSGVPGVRVEPLTGLLVDFCRERDAGYVVKGLRFASDFDFELQMAH
                     INHRLTGLETVLLPASAQWGTLSSTMVREVATLGGDVRAFVPDVVARSIEQKRDSHHG
                     "
     gene            1524..2021
                     /locus_tag="PLF56_13270"
     CDS             1524..2021
                     /locus_tag="PLF56_13270"
                     /inference="COORDINATES: ab initio
                     prediction:GeneMarkS-2+"
                     /note="Derived by automated computational analysis using
                     gene prediction method: GeneMarkS-2+."
                     /codon_start=1
                     /transl_table=11
                     /product="hypothetical protein"
                     /protein_id="HOQ54568.1"
                     /translation="MAESTADRLDALHTLVQQAKAVPISASCVVNRAEVLGLIEAAQA
                     SLVQELAAAKDVANASPPAIERAQEQADQIVRAAEKKAAYLVDSSQVLTAARDRAGQL
                     ESRAIAESEGLRREADVYVDGRIAAMEAGLQKQLTQLQTIRARLATRSGLDADETTTL
                     PKVTG"
BASE COUNT          350 a          716 c          836 g          362 t
ORIGIN      
        1 gtccacctcg cgctcgggct cgtcgccgaa cacgaaccgg cgcccttcga tctggtcgac
       61 ggcgatacgg atgaagtggg tcttcagcgt cggtacccac ggccgcagcg gcaactgctc
      121 gacgcgggcc aggtcgtccc actcggtcaa ctcctgtgcg tgacccttga ccaccacgct
      181 ccagcccgtc gactccgtcc acgaatcgac ttcgaaggcg acgcccgagt tgacgatcaa
      241 ctgcagcagc ttcgaaccct cggcggtgcg aaagaccacc gcgggaccgt ccagcacgaa
      301 gttgaccgga aagatgtcgg gctggcgttc gatgacggtg gcgaggcggc ccacctgcgc
      361 gctgccgagg agttgccagg cctcggcctc atccagccgc gtgatcgggg gtcggttagc
      421 catacctgat tgtgtcactg ggtggccgtg cgggcgtggg ctacggtcaa tccatgtcgc
      481 ggatcatcgc cggtagtcgt aagggccaac ggttgctgac tcccgcgggg gacgcgaccc
      541 ggccgacatc cgatcgggtg cgcgaggccg ccttcggggc gctcgcttcg gaactcggac
      601 gggcgggcga cccggcgtcc atgctggagg gctgcggctt cctcgatctg ttcgccggtt
      661 cgggagcggt ggcgctcgag gcggcgagcc gtggcgccgc gcccgtggtg gcggtcgaga
      721 aggaacgtcg agcggtcgag gtgattcggc gcaacgtgca gcagaccggg ttgggggtgc
      781 gggtggtcag cgccggtgtt ggtgcctttc tggggggcga gccgacgccc ttccggttgt
      841 gctggctcga cccgccctac gaactcggca acgagcggat cgagccactg gtgtctcgcc
      901 tggtgcaggg gtggcttgca ccgggcggca tcgtggtggt cgaacgggcg cgccgcagcg
      961 gtgcgttcga gtggcccgag cagttggggg atcgctggca tcgtcgctac ggtgagacga
     1021 cgttgttctt cgcgacggaa ggtgaggcat gagggcggtc tgccccggat cgtacgatcc
     1081 gatcacccac ggtcatctga acatcatcga gcgggccgcg gcgctcttcg gcgacgtgat
     1141 agttgcggtc ggacgcaact cggccaaaag ttacctcttc gacaccgacg agcgggtcga
     1201 gctggcgcgg gccgtcacgt ccggggtgcc gggggtgcgc gtcgagccgt tgaccgggtt
     1261 gttggtggac ttctgccgtg aacgcgacgc cggctacgtg gtgaaggggc tgcggttcgc
     1321 gtccgatttc gacttcgagt tgcagatggc gcacatcaat caccgattga ccggactgga
     1381 gacggtgctg ctgccggctt cggcacaatg gggcacgttg tcgtccacga tggtgcgtga
     1441 ggtggcgacg ctgggtggcg acgtgcgggc cttcgtgcct gatgtggtcg cccgatccat
     1501 cgaacagaaa agagactccc atcatggctg aatcgaccgc cgaccggctg gacgcgctgc
     1561 acaccctggt ccagcaggcg aaggcggtgc ctatctcggc gtcctgcgtg gtcaaccggg
     1621 cagaggtgct cggcctgatc gaggctgccc aagcgtcact ggtgcaggag ctggccgcgg
     1681 cgaaggacgt cgccaacgcc agcccgccgg cgatcgaacg ggcgcaggag caggccgacc
     1741 agatcgtccg ggcggcggag aagaaggcgg catacctggt ggattcgagc caggtgctga
     1801 ccgcggcccg cgaccgcgcg ggccagctcg agagccgggc gatcgccgaa tcggaggggc
     1861 tgcgccgcga ggccgacgtg tacgtggacg gccgcatcgc cgccatggag gccggcctgc
     1921 agaagcagct cacccagctt cagacgatcc gcgcgcggct ggccacccgt tcgggcctgg
     1981 acgcggacga aaccaccacg ctgcccaagg tgaccggctg accgtccgct tccgggacgg
     2041 ttctccgcat ggttcgggac ggtcccggca cggcgccgga tgggtcgacg tcgcaccgac
     2101 caaggcaggt gaggccggac gggacggtgg agaaccgtac ccgaaccggt tgggggccgt
     2161 ctccgagacc cgagcgagca ccgtccgcga accggtcggg aaccgtgcgt caccgccgat
     2221 tggcgccgtc gcaggcagcc cggtaaagta tcccttcggt catg
//
LOCUS       DAOSUG010000499         2246 bp    DNA     linear   ENV 10-JUN-2024
DEFINITION  MAG TPA_asm: Micropruina sp. isolate AMR_MDS_2195
            NODE_19547_length_2246_cov_3.679598, whole genome shotgun sequence.
ACCESSION   DAOSUG010000499 DAOSUG010000000
VERSION     DAOSUG010000499.1
DBLINK      BioProject: PRJNA925477
            BioSample: SAMN32864262
            Sequence Read Archive: SRR4236673
KEYWORDS    WGS; ENV; Metagenome Assembled Genome; MAG; Third Party Data; TPA;
            TPA:assembly.
SOURCE      Micropruina sp. (wastewater metagenome)
  ORGANISM  Micropruina sp.
            Bacteria; Bacillati; Actinomycetota; Actinomycetes;
            Propionibacteriales; Nocardioidaceae; Micropruina.
REFERENCE   1  (bases 1 to 2246)
  AUTHORS   Abdulkadir,N., Saraiva,J.P., Zhang,J., Stolte,S., Gillor,O.,
            Harms,H. and Rocha,U.
  TITLE     Genome-centric analyses of 165 metagenomes show that mobile genetic
            elements are crucial for the transmission of antimicrobial
            resistance genes to pathogens in activated sludge and wastewater
  JOURNAL   Microbiol Spectr 12 (3), e0291823 (2024)
   PUBMED   38289113
REFERENCE   2  (bases 1 to 2246)
  AUTHORS   Abdulkadir,N.
  TITLE     Direct Submission
  JOURNAL   Submitted (24-MAR-2023) Environmental Microbiology, Helmholtz
            Center for Environmental Research -UFZ, Permoserstr 15, Leipzig
            04318, Germany
COMMENT     The annotation was added by the NCBI Prokaryotic Genome Annotation
            Pipeline (PGAP). Information about PGAP can be found here:
            https://www.ncbi.nlm.nih.gov/genome/annotation_prok/
            
            ##Genome-Assembly-Data-START##
            Assembly Method        :: SPAdes v. 3.11.1
            Genome Representation  :: Full
            Expected Final Version :: Yes
            Genome Coverage        :: Not Applicable
            Sequencing Technology  :: Illumina MiSeq
            ##Genome-Assembly-Data-END##
            
            ##Genome-Annotation-Data-START##
            Annotation Provider               :: NCBI
            Annotation Date                   :: 05/04/2023 04:57:10
            Annotation Pipeline               :: NCBI Prokaryotic Genome
                                                 Annotation Pipeline (PGAP)
            Annotation Method                 :: Best-placed reference protein
                                                 set; GeneMarkS-2+
            Annotation Software revision      :: 6.5
            Features Annotated                :: Gene; CDS; rRNA; tRNA; ncRNA
            Genes (total)                     :: 2,776
            CDSs (total)                      :: 2,750
            Genes (coding)                    :: 2,714
            CDSs (with protein)               :: 2,714
            Genes (RNA)                       :: 26
            tRNAs                             :: 24
            ncRNAs                            :: 2
            Pseudo Genes (total)              :: 36
            CDSs (without protein)            :: 36
            Pseudo Genes (ambiguous residues) :: 0 of 36
            Pseudo Genes (frameshifted)       :: 2 of 36
            Pseudo Genes (incomplete)         :: 32 of 36
            Pseudo Genes (internal stop)      :: 2 of 36
            ##Genome-Annotation-Data-END##
FEATURES             Location/Qualifiers
     source          1..2246
                     /organism="Micropruina sp."
                     /mol_type="genomic DNA"
                     /submitter_seqid="NODE_19547_length_2246_cov_3.679598"
                     /isolate="AMR_MDS_2195"
                     /isolation_source="Activated sludge of municipal
                     wastewater treatment plants"
                     /db_xref="taxon:2737536"
                     /environmental_sample
                     /geo_loc_name="Canada"
                     /lat_lon="49.25 N 123.1 W"
                     /collection_date="2015-06-16"
                     /metagenome_source="wastewater metagenome"
                     /note="metagenomic"
     gene            <1..191
                     /locus_tag="PLF56_13275"
     CDS             <1..191
                     /locus_tag="PLF56_13275"
                     /inference="COORDINATES: ab initio
                     prediction:GeneMarkS-2+"
                     /note="Derived by automated computational analysis using
                     gene prediction method: GeneMarkS-2+."
                     /codon_start=3
                     /transl_table=11
                     /product="hypothetical protein"
                     /protein_id="HOQ54569.1"
                     /translation="NLQYPYDYPASGASEKGIDVAIAVDMIRLGMSGYMDAALLFSLR
                     QRPHARRRSALADAELSR"
     gene            656..946
                     /locus_tag="PLF56_13280"
     CDS             656..946
                     /locus_tag="PLF56_13280"
                     /inference="COORDINATES: protein motif:HMM:NF012752.2"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_function: GO:0003735 - structural constituent of
                     ribosome [Evidence IEA];
                     GO_process: GO:0006412 - translation [Evidence IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="ribosomal protein L7/L12"
                     /protein_id="HOQ54570.1"
                     /translation="MGIFGNDSGELENRVRLLEDQVTQLWRIVQAQQGDDPVPTEGFE
                     QPTWVQPDSDDPPWLPEVRQLVAANRKIEAIKQVREHTGWGLKEAKNYVDRL"
     gene            complement(975..2039)
                     /locus_tag="PLF56_13285"
     CDS             complement(975..2039)
                     /locus_tag="PLF56_13285"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_012919386.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="PfkB family carbohydrate kinase"
                     /protein_id="HOQ54571.1"
                     /translation="MTGRRFDVLVAGMVFLDVVFTELPTPPSPGTEVWAAGMGSSPGG
                     IANLAVALSRLGLHTGLIAGFGDDGYGEWCRRVLSDQEGIDTSMSPSFEGWHTPVTVS
                     MAYAGDRAMVTHGHEAPIPASRLMRPGTAARAVIAELDGDPWWRPLAADGALVFADVG
                     WDGTGAWDPCILDRLAGCHCFMPNDREAMAYTRTDTAEEALELLADLVPCAVVTLGAQ
                     GAIAVDGRTGEWARVRALPVRAIDPTGAGDCFGAALVAGTLAGWPLTVRLRFAALTAG
                     LAVQQFGGALATPGWGDVADWWTQQRALAAQGDALARELVADYDFLPSIMPAGEPARV
                     RRADATIAKFSHLPTSGTRS"
BASE COUNT          383 a          818 c          730 g          315 t
ORIGIN      
        1 gtaaccttca gtacccctac gactacccgg catcaggagc ctcggagaaa ggcatcgacg
       61 tagcgatcgc cgtggatatg atccgtctgg ggatgtccgg ctacatggat gccgcgcttc
      121 tattctccct ccgacaacga cctcatgccc gccgtcgaag tgctctggca gatgccgaac
      181 tgtcacgttg aagttgcctc ctggtcaggc gcgcaccgcg tgcgcttccc agggacccag
      241 cagccctggt gccaccacct cggcgaagcc gactttcagg cggtgcgcga cacttacgac
      301 tactcgatcc gcgagtaacc ctcgaacatg cggacgctaa tcgacgctaa tcgggtcttt
      361 gcccagactg tgccccggtg agcgccgaat cgccgcgcca gggcggcgac gctgacgccg
      421 ttggcacggg ctgttcgcat agcgtccact tcctcgtcgg tcagctgtgt ccgaggtcgt
      481 ctcctttgtt cggccgacgc ctcgatgagc acatcatcgg gctggtcagc agactcgccc
      541 gtacccccag acccttgatt ccattcggaa actagccgct caacccgctg tcgcctgttc
      601 ccacagcgtt cgatggcgtc caccggacgg actggtgccg ccctacgctg cgagcatggg
      661 catcttcggc aacgactccg gcgaactcga gaaccgcgtc cgcctcctgg aggaccaggt
      721 gacgcagctg tggcgcatcg tccaggcgca gcagggcgac gatccggtac ccacggaggg
      781 attcgagcag cccacctggg tacagccgga ctcggacgac ccgccatggc tgccagaggt
      841 tcgccaactg gtcgccgcca accgaaagat cgaggcgatc aaacaggtgc gtgagcacac
      901 cgggtggggc ctcaaggagg cgaagaacta cgtggaccgg ctctgaccct gagccccgcg
      961 ccgaacgagc aaggtcacga tcgggtgccg ctggtcggca ggtgcgagaa cttcgcgatg
     1021 gtggcgtccg cgcgacgcac ccgggcaggc tccccggccg gcatgatcga cggcagaaag
     1081 tcgtagtcgg cgaccagttc gcgggcaagc gcatcaccct gtgccgccaa ggcccgctgc
     1141 tgcgtccacc agtccgcgac gtcaccccag ccgggggtgg ccagggcacc gccgaactgc
     1201 tgcacagcca gcccggcggt caacgccgcg aaccgcagcc gcaccgtcag cggccagccg
     1261 gccagggtgc ccgccaccag cgcggcgccg aaacagtcac cggcgccggt cggatcgatc
     1321 gcccgcaccg gcaacgcccg gacccgtgcc cactcgccgg tgcgtccgtc cacggcgatc
     1381 gccccctggg cgcccagcgt caccacggcg cagggcacca ggtcggcgag tagctccagt
     1441 gcctcctcgg cggtgtccgt gcgggtgtag gccatcgcct cccggtcgtt gggcatgaag
     1501 cagtgacagc cggcgagccg gtcgagaatg caggggtccc aggcgccggt gccgtcccag
     1561 cccacgtccg cgaacaccag cgcgccgtcg gcggccaggg gccgccacca gggatcaccg
     1621 tcgagttcgg cgatcaccgc gcgggccgcg gtgcccggcc gcatcagccg ggacgcgggg
     1681 atcggggcct catgcccatg ggtcaccatg gcgcggtcac cggcgtacgc catcgagacc
     1741 gtcaccgggg tgtgccagcc ctcgaaactg ggcgacatcg aggtatcgat tccctcttgg
     1801 tcgctcaaca cccgccgaca ccattcgccg tagccgtcgt cgccgaaacc ggcgatcagc
     1861 ccggtgtgga ggcccagccg cgacagcgcg acggccaggt tcgcgatgcc gcccggggac
     1921 gagcccatac cggcggccca cacctcggtg ccgggtgagg gcggggtggg tagttcggtg
     1981 aacacgacgt ccagaaacac catgcccgcg accagcacgt cgaaccgccg accggtcaac
     2041 gcggcgtccg atcgtcgatg ctcacagctg atccgtcgcg gcgttccagg tggcggcgat
     2101 ccgcgcccgc cgacgccgcg caggacgcac cgcgatcgcg acggcggtga ccagcaccgc
     2161 aacaccgagc acaagcttca cgggtgcgag tgtaacgacg atcatcggtg agcccctccc
     2221 caggaccgga cgccccggcg cgcctc
//
LOCUS       DAOSUG010000500         2237 bp    DNA     linear   ENV 10-JUN-2024
DEFINITION  MAG TPA_asm: Micropruina sp. isolate AMR_MDS_2195
            NODE_19640_length_2237_cov_4.301558, whole genome shotgun sequence.
ACCESSION   DAOSUG010000500 DAOSUG010000000
VERSION     DAOSUG010000500.1
DBLINK      BioProject: PRJNA925477
            BioSample: SAMN32864262
            Sequence Read Archive: SRR4236673
KEYWORDS    WGS; ENV; Metagenome Assembled Genome; MAG; Third Party Data; TPA;
            TPA:assembly.
SOURCE      Micropruina sp. (wastewater metagenome)
  ORGANISM  Micropruina sp.
            Bacteria; Bacillati; Actinomycetota; Actinomycetes;
            Propionibacteriales; Nocardioidaceae; Micropruina.
REFERENCE   1  (bases 1 to 2237)
  AUTHORS   Abdulkadir,N., Saraiva,J.P., Zhang,J., Stolte,S., Gillor,O.,
            Harms,H. and Rocha,U.
  TITLE     Genome-centric analyses of 165 metagenomes show that mobile genetic
            elements are crucial for the transmission of antimicrobial
            resistance genes to pathogens in activated sludge and wastewater
  JOURNAL   Microbiol Spectr 12 (3), e0291823 (2024)
   PUBMED   38289113
REFERENCE   2  (bases 1 to 2237)
  AUTHORS   Abdulkadir,N.
  TITLE     Direct Submission
  JOURNAL   Submitted (24-MAR-2023) Environmental Microbiology, Helmholtz
            Center for Environmental Research -UFZ, Permoserstr 15, Leipzig
            04318, Germany
COMMENT     The annotation was added by the NCBI Prokaryotic Genome Annotation
            Pipeline (PGAP). Information about PGAP can be found here:
            https://www.ncbi.nlm.nih.gov/genome/annotation_prok/
            
            ##Genome-Assembly-Data-START##
            Assembly Method        :: SPAdes v. 3.11.1
            Genome Representation  :: Full
            Expected Final Version :: Yes
            Genome Coverage        :: Not Applicable
            Sequencing Technology  :: Illumina MiSeq
            ##Genome-Assembly-Data-END##
            
            ##Genome-Annotation-Data-START##
            Annotation Provider               :: NCBI
            Annotation Date                   :: 05/04/2023 04:57:10
            Annotation Pipeline               :: NCBI Prokaryotic Genome
                                                 Annotation Pipeline (PGAP)
            Annotation Method                 :: Best-placed reference protein
                                                 set; GeneMarkS-2+
            Annotation Software revision      :: 6.5
            Features Annotated                :: Gene; CDS; rRNA; tRNA; ncRNA
            Genes (total)                     :: 2,776
            CDSs (total)                      :: 2,750
            Genes (coding)                    :: 2,714
            CDSs (with protein)               :: 2,714
            Genes (RNA)                       :: 26
            tRNAs                             :: 24
            ncRNAs                            :: 2
            Pseudo Genes (total)              :: 36
            CDSs (without protein)            :: 36
            Pseudo Genes (ambiguous residues) :: 0 of 36
            Pseudo Genes (frameshifted)       :: 2 of 36
            Pseudo Genes (incomplete)         :: 32 of 36
            Pseudo Genes (internal stop)      :: 2 of 36
            ##Genome-Annotation-Data-END##
FEATURES             Location/Qualifiers
     source          1..2237
                     /organism="Micropruina sp."
                     /mol_type="genomic DNA"
                     /submitter_seqid="NODE_19640_length_2237_cov_4.301558"
                     /isolate="AMR_MDS_2195"
                     /isolation_source="Activated sludge of municipal
                     wastewater treatment plants"
                     /db_xref="taxon:2737536"
                     /environmental_sample
                     /geo_loc_name="Canada"
                     /lat_lon="49.25 N 123.1 W"
                     /collection_date="2015-06-16"
                     /metagenome_source="wastewater metagenome"
                     /note="metagenomic"
     gene            158..391
                     /gene="nrdH"
                     /locus_tag="PLF56_13290"
     CDS             158..391
                     /gene="nrdH"
                     /locus_tag="PLF56_13290"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_017202656.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_function: GO:0097573 - glutathione oxidoreductase
                     activity [Evidence IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="glutaredoxin-like protein NrdH"
                     /protein_id="HOQ54572.1"
                     /translation="MAITVYSKPSCVQCTATYRALDKLGLDYDVVDLSADEAALTDVL
                     ATGYQQAPVVVAGDEHWAGFRPDLIKAAAGMAA"
     gene            400..834
                     /gene="nrdI"
                     /locus_tag="PLF56_13295"
     CDS             400..834
                     /gene="nrdI"
                     /locus_tag="PLF56_13295"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_009212695.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_process: GO:0009263 - deoxyribonucleotide biosynthetic
                     process [Evidence IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="class Ib ribonucleoside-diphosphate reductase
                     assembly flavoprotein NrdI"
                     /protein_id="HOQ54573.1"
                     /translation="MAALVYFSSVSENTHRFVGKLGLPADRIPVLPGEPHLTASEAFV
                     LVLPTYGGGNGAGAVPKQVIRFLNDPGNRTLLRGVISAGNTNFGAAYCIAGEIVARKC
                     HVPHLYQFELMGTPDDVEAVREGLTSQWLPPFSTNHSSTTPA"
     gene            786..>2237
                     /gene="nrdE"
                     /locus_tag="PLF56_13300"
     CDS             786..>2237
                     /gene="nrdE"
                     /locus_tag="PLF56_13300"
                     /EC_number="1.17.4.1"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_019126614.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_function: GO:0004748 - ribonucleoside-diphosphate
                     reductase activity, thioredoxin disulfide as acceptor
                     [Evidence IEA];
                     GO_function: GO:0005524 - ATP binding [Evidence IEA];
                     GO_process: GO:0006260 - DNA replication [Evidence IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="class 1b ribonucleoside-diphosphate reductase
                     subunit alpha"
                     /protein_id="HOQ54574.1"
                     /translation="MATTLFHEPQLNDTGVAAAPAEVDYHALNALLNLYDADGRIQFE
                     ADRQAAHQYFLQHVNQNTVFFHSLKEKLDYLIEQGYYEPQVLAHYDFDYVKSLFKRAY
                     AVRFRFPTFMGAFKYYTSYTLKTFDGSRYLERFEDRVCMVALTLAAGDRGLAEQIVDE
                     VMAGRFQPATPTFLNAGKASRGELVSCFLLRVEDNMESIARAINSSLQLSKRGGGVAL
                     SLTNIREAGAPIKKIQNQSSGVIPVMKLLEDSFSYANQLGARQGAGAVYLNAHHPDIL
                     SFLDTKRENADEKIRIKTLSLGVVIPDITFELARDNEDMYLFSPYDIERVYGMPFTEI
                     SVTQKYREMVDDRRITKRRINARTFFQTLAEIQFESGYPYLVFEDTVNRANPIDGRIT
                     MSNLCSEILQVSTPSTFNEDLSYASVGKDISCNLGSLNIAKAMDSPHFGRTIETAIRA
                     LTAVSDQSDIACAPSIERGNAMSHAIGLGAMNLH"
BASE COUNT          412 a          777 c          680 g          368 t
ORIGIN      
        1 atcgtctcgg ggttgccgag ggtgcgcagc gtcgctcggc tcagcctcgc gggagggttg
       61 cgtgccacgt gggccatcgg tccgcgggca ggtccgtccc tcgtcggttg cgcaccagca
      121 gttcctctcg acgatcggac ggaaaggacc agccatcatg gcgatcaccg tttacagcaa
      181 gccctcgtgc gtgcagtgca ccgcgaccta ccgggcactc gacaagctcg gcctcgacta
      241 cgacgtcgtc gacctcagcg ccgacgaggc cgccctcacc gacgtgctgg ccacgggcta
      301 tcagcaggcg ccggtggtcg ttgccggcga cgaacactgg gccgggttcc gtcccgacct
      361 gatcaaggcg gcggccggca tggctgcctg accgcggcca tggccgcgct ggtctacttc
      421 tcgtcggtct ccgagaacac ccaccgcttc gtcggcaaac tcggtctgcc cgccgaccgc
      481 attccggtgc tgccgggcga gccgcacctg acggcgtcag aggcgttcgt gctggtgctg
      541 cccacctacg gcggcggcaa cggggcgggc gcggtgccca agcaggtgat caggtttctc
      601 aacgaccccg gcaaccggac gctgctacgc ggcgtgatca gcgccggcaa caccaacttc
      661 ggcgccgcct actgcatcgc cggcgagatc gtggcccgca agtgccacgt tccgcacctc
      721 taccaattcg aactgatggg cacgcccgac gacgtcgagg cggtccgcga agggctcaca
      781 tcgcaatggc taccaccctt ttccacgaac cacagctcaa cgacaccggc gtagcagccg
      841 cgcccgccga ggtcgactac cacgcgctga acgcgctgct caacctctac gacgcggacg
      901 gccgcatcca gttcgaggcc gaccggcagg ccgcgcacca gtactttctt cagcacgtca
      961 accagaacac cgtgttcttc cactcgctga aggagaaact cgattacctc atcgagcagg
     1021 gctactacga accgcaggtg ctggcccact acgacttcga ctacgtcaag tcgctgttca
     1081 agcgtgctta cgcggtcagg ttccgctttc ccaccttcat gggggcgttc aagtactaca
     1141 ccagctacac cttgaagacg ttcgacggca gccgctacct ggaacgcttc gaagaccggg
     1201 tctgcatggt cgcgctcacc ctcgcggccg gcgaccgtgg gctggccgag cagatcgtcg
     1261 acgaggtgat ggccggacgc ttccagcccg ccaccccgac gttcctcaac gccggcaagg
     1321 cgtcacgggg cgaactggtg tcgtgctttc tgctgcgggt ggaggacaac atggagtcga
     1381 tcgcccgggc gatcaactcc tcgctgcaac tgtccaagcg gggcggtggc gtggcgttgt
     1441 cgctgaccaa catccgcgag gccggcgcgc cgatcaagaa gatccagaac cagagctccg
     1501 gggtgattcc ggtgatgaag ctgctggagg actcgttcag ctacgccaat cagctgggcg
     1561 cccggcaggg agcgggcgcg gtgtacctca acgcgcacca ccccgacatt ctcagctttc
     1621 tcgataccaa gcgcgagaac gccgacgaga agatccggat caagaccctc agcctgggcg
     1681 tggtcattcc cgacatcacc ttcgaactgg cccgcgacaa cgaggacatg tacctgttca
     1741 gtccctatga catcgagcgg gtctacggga tgccgttcac cgagatctcg gtgacgcaga
     1801 agtaccgcga gatggtggac gacaggcgca tcaccaagcg caggatcaac gctcgcacgt
     1861 tctttcagac gctggccgag attcagttcg agtcgggcta cccctacctg gtcttcgagg
     1921 acacggtgaa ccgggcgaat ccgatcgacg gccggatcac catgtcgaac ctgtgctccg
     1981 agattctgca ggtctccacc ccgtccacct tcaacgagga cctttcgtac gcgtcggtcg
     2041 gcaaggacat cagctgcaac ctcggctcgc tgaacatcgc caaggcgatg gactcgccgc
     2101 acttcggccg caccatcgag accgcgatcc gggcgctcac cgcggtctcc gaccagtccg
     2161 acatcgcctg cgcacccagc atcgagcgcg gcaacgcgat gagccacgcg atcgggctcg
     2221 gcgcgatgaa cctgcac
//
LOCUS       DAOSUG010000501         2237 bp    DNA     linear   ENV 10-JUN-2024
DEFINITION  MAG TPA_asm: Micropruina sp. isolate AMR_MDS_2195
            NODE_19641_length_2237_cov_4.254812, whole genome shotgun sequence.
ACCESSION   DAOSUG010000501 DAOSUG010000000
VERSION     DAOSUG010000501.1
DBLINK      BioProject: PRJNA925477
            BioSample: SAMN32864262
            Sequence Read Archive: SRR4236673
KEYWORDS    WGS; ENV; Metagenome Assembled Genome; MAG; Third Party Data; TPA;
            TPA:assembly.
SOURCE      Micropruina sp. (wastewater metagenome)
  ORGANISM  Micropruina sp.
            Bacteria; Bacillati; Actinomycetota; Actinomycetes;
            Propionibacteriales; Nocardioidaceae; Micropruina.
REFERENCE   1  (bases 1 to 2237)
  AUTHORS   Abdulkadir,N., Saraiva,J.P., Zhang,J., Stolte,S., Gillor,O.,
            Harms,H. and Rocha,U.
  TITLE     Genome-centric analyses of 165 metagenomes show that mobile genetic
            elements are crucial for the transmission of antimicrobial
            resistance genes to pathogens in activated sludge and wastewater
  JOURNAL   Microbiol Spectr 12 (3), e0291823 (2024)
   PUBMED   38289113
REFERENCE   2  (bases 1 to 2237)
  AUTHORS   Abdulkadir,N.
  TITLE     Direct Submission
  JOURNAL   Submitted (24-MAR-2023) Environmental Microbiology, Helmholtz
            Center for Environmental Research -UFZ, Permoserstr 15, Leipzig
            04318, Germany
COMMENT     The annotation was added by the NCBI Prokaryotic Genome Annotation
            Pipeline (PGAP). Information about PGAP can be found here:
            https://www.ncbi.nlm.nih.gov/genome/annotation_prok/
            
            ##Genome-Assembly-Data-START##
            Assembly Method        :: SPAdes v. 3.11.1
            Genome Representation  :: Full
            Expected Final Version :: Yes
            Genome Coverage        :: Not Applicable
            Sequencing Technology  :: Illumina MiSeq
            ##Genome-Assembly-Data-END##
            
            ##Genome-Annotation-Data-START##
            Annotation Provider               :: NCBI
            Annotation Date                   :: 05/04/2023 04:57:10
            Annotation Pipeline               :: NCBI Prokaryotic Genome
                                                 Annotation Pipeline (PGAP)
            Annotation Method                 :: Best-placed reference protein
                                                 set; GeneMarkS-2+
            Annotation Software revision      :: 6.5
            Features Annotated                :: Gene; CDS; rRNA; tRNA; ncRNA
            Genes (total)                     :: 2,776
            CDSs (total)                      :: 2,750
            Genes (coding)                    :: 2,714
            CDSs (with protein)               :: 2,714
            Genes (RNA)                       :: 26
            tRNAs                             :: 24
            ncRNAs                            :: 2
            Pseudo Genes (total)              :: 36
            CDSs (without protein)            :: 36
            Pseudo Genes (ambiguous residues) :: 0 of 36
            Pseudo Genes (frameshifted)       :: 2 of 36
            Pseudo Genes (incomplete)         :: 32 of 36
            Pseudo Genes (internal stop)      :: 2 of 36
            ##Genome-Annotation-Data-END##
FEATURES             Location/Qualifiers
     source          1..2237
                     /organism="Micropruina sp."
                     /mol_type="genomic DNA"
                     /submitter_seqid="NODE_19641_length_2237_cov_4.254812"
                     /isolate="AMR_MDS_2195"
                     /isolation_source="Activated sludge of municipal
                     wastewater treatment plants"
                     /db_xref="taxon:2737536"
                     /environmental_sample
                     /geo_loc_name="Canada"
                     /lat_lon="49.25 N 123.1 W"
                     /collection_date="2015-06-16"
                     /metagenome_source="wastewater metagenome"
                     /note="metagenomic"
     gene            complement(277..591)
                     /locus_tag="PLF56_13305"
     CDS             complement(277..591)
                     /locus_tag="PLF56_13305"
                     /inference="COORDINATES: ab initio
                     prediction:GeneMarkS-2+"
                     /note="Derived by automated computational analysis using
                     gene prediction method: GeneMarkS-2+."
                     /codon_start=1
                     /transl_table=11
                     /product="hypothetical protein"
                     /protein_id="HOQ54575.1"
                     /translation="MARQVGTQPEHQGGIDVPDAETGSQGIGGLHGKGVRIGHAATVS
                     RRSWPPGGPAVLAEESAVSVHGAIDEGRFGVEMIERGRSGHAGSGSRPARGQGLKQGE
                     GL"
     gene            complement(615..1052)
                     /locus_tag="PLF56_13310"
     CDS             complement(615..1052)
                     /locus_tag="PLF56_13310"
                     /inference="COORDINATES: ab initio
                     prediction:GeneMarkS-2+"
                     /note="Derived by automated computational analysis using
                     gene prediction method: GeneMarkS-2+."
                     /codon_start=1
                     /transl_table=11
                     /product="hypothetical protein"
                     /protein_id="HOQ54576.1"
                     /translation="MRSRVIRLGSLALGTLFVASSLVAVAPSAEAASPCPGKRVGLYQ
                     LYAGGEVADHFKHGQIEVWYSSAKGGTNCVLVRDNTPGKHKMMVEASISGRSSAGKDS
                     GTYNYYAGPIRLTKTNGKCISVYAEVKSDGISYRRALNKKHCG"
     gene            1251..1838
                     /locus_tag="PLF56_13315"
     CDS             1251..1838
                     /locus_tag="PLF56_13315"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_002514726.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="uridine kinase"
                     /protein_id="HOQ54577.1"
                     /translation="MERRLVLVAGPSGSGKSRLTLASGLPQVRLDDFYHDADHPGLPR
                     TLGIPDWDDPATWNGDAAIAALGQLLATGRTHTPDYSIPLSRKVGDKNLAIPDDAPVV
                     LAEGIFAIKALPLARAAGLDASGLYLNRPGAQVAYFRLKRDLKQRRKPPWVLVRRGLA
                     LWRAQDELKRKAIANGFRAVSYAEAEGLLLPRASG"
     gene            complement(1947..>2237)
                     /locus_tag="PLF56_13320"
     CDS             complement(1947..>2237)
                     /locus_tag="PLF56_13320"
                     /EC_number="6.1.1.10"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_016669487.1"
                     /note="methionine--tRNA ligase; MetRS; adds methionine to
                     tRNA(Met) with cleavage of ATP to AMP and diphosphate;
                     some MetRS enzymes form dimers depending on a C-terminal
                     domain that is also found in other proteins such as
                     Trbp111 in Aquifex aeolicus and the cold-shock protein
                     CsaA from Bacillus subtilis while others do not; four
                     subfamilies exist based on sequence motifs and zinc
                     content; Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="methionine--tRNA ligase"
                     /protein_id="HOQ54578.1"
                     /translation="QDCNTLLTPLLPHAAQKVFEALGGEGVWAAQPELVEVTEPGADA
                     PYPVLRGDYVNQQAVWRSTPIAVGTPLAKPTPIFTKLDPKLAETGPEWAPIG"
BASE COUNT          340 a          798 c          747 g          352 t
ORIGIN      
        1 gacgagactg tggacgacta ggacgtctcc gctcgggcgt cagcaaccac gtcgcccgcg
       61 ctcgcgctac ccaccgtccg ccgcgccgat gtctgccgga gcagcggcgc acgcgtcatc
      121 ccgcgcgtgc cccgggatcc cggcacaggc aaatggtccc tacccctggg gctcgatgac
      181 ggggttacgg cgtgtgcgaa acgctcctca cgtggtgatg gcgtcgtgcc atgcgcaggg
      241 tgtgaacgac gtgacggact gtgatccgat gtcggatcac agtccttcac cttgtttgag
      301 accctggccg cgggccggtc gtgaaccgga tccggcatgg ccggatcgcc cgcgctcgat
      361 catctcgacc ccaaatcgac cctcgtcgat tgctccgtgg accgagaccg cactctcctc
      421 agcgagcacc gccggcccgc ccgggggcca tgacctgcgc gatacagtcg ccgcatggcc
      481 gatccgtacg cccttgccgt gcaggccgcc gatgccctgc gatcccgttt cggcatcggg
      541 cacatcgatt ccgccctggt gctccggttg cgtgccgacc tggcgggcca cgctggggct
      601 ctgagcgagg accgctaccc gcagtgcttc ttgttcaggg cacggcggta cgagatgccg
      661 tcgctcttca cctcggcgta caccgagatg cacttgccgt tggtcttcgt gaggcggatc
      721 gggcccgcgt agtagttgta ggtgccgctg tccttgcccg ccgacgatcg gcccgagatg
      781 ctggcctcca ccatcatctt gtgcttgccg ggggtgttgt cgcggaccag cacgcagttg
      841 gtgccgccct tggccgatga gtaccagacc tcgatctgac cgtgcttgaa gtggtccgcc
      901 acctcaccgc cggcgtacag ctggtacaga ccgacccgct tgcccgggca cggcgacgcc
      961 gcctcggcgg acggggcgac cgcgaccagg gacgaggcga cgaacagcgt gccgagcgct
     1021 agcgaaccaa gacgaatgac ccgggacctc atgatgacca acctttgcgt gcgggcgaaa
     1081 tgccgacact gtagacgatg gccggccccc ctttcaccgc tggcccgaga tgaccgtccg
     1141 acgggccgtg gggacgagca gggtcggggc ggccgccgcg tcaaccaccg tgcggccgga
     1201 agcggctcag ccacccctga gccgctggac gacccctaca ctgagcgccc atggagcgac
     1261 gcctggtgct ggtggcagga ccctccggca gcggcaagtc gcggctgacg ctggcatccg
     1321 ggctgccgca ggtgcggctg gacgacttct accacgacgc cgaccaccct ggcctgccgc
     1381 gcaccctcgg catacccgat tgggacgacc ctgcgacctg gaacggcgac gctgcgatcg
     1441 cggcgctggg ccaactgctc gccaccggac gcacccacac ccccgactac tcgatcccgc
     1501 tgtcacgcaa ggtcggtgac aagaacctgg cgattcccga tgacgccccg gtggtgctcg
     1561 ccgagggcat cttcgcgatc aaagctctgc cgctggcgcg agccgcgggc ctggatgcgt
     1621 ccggcctcta tctgaaccgc ccgggcgcgc aggtggccta cttcaggctc aagcgtgacc
     1681 tgaagcaacg gcgcaagcca ccctgggtgc tggtgcggcg tgggctggcg ttgtggcgcg
     1741 ctcaggatga actcaaacgc aaggccatcg ccaacggctt ccgagcggtg agttatgccg
     1801 aggcggaagg actgctgcta ccccgggcgt ccggctgagg agcgccgctc cacgacaccc
     1861 tcgccgagcg cccccaagta tttctggccc gcgaagcttc gcgggccaga gatacttagg
     1921 gaggcgttgg gtggaccggc cgcggctcag ccgatcggcg cccactccgg cccggtctcg
     1981 gccagcttcg ggtcgagctt ggtgaagatc ggggtcggct tggccagcgg cgtgcccacg
     2041 gcgatgggcg tcgaccgcca caccgcctgc tggttcacgt agtcaccgcg caacaccggg
     2101 tacggcgcgt ccgcccccgg ctcggtgacc tcgaccagtt cgggctgcgc ggcccacaca
     2161 ccctcaccgc cgagcgcctc gaacaccttc tgcgcggcgt gcggcagcag cggggtgagc
     2221 agggtgttgc agtcctg
//
LOCUS       DAOSUG010000502         2237 bp    DNA     linear   ENV 10-JUN-2024
DEFINITION  MAG TPA_asm: Micropruina sp. isolate AMR_MDS_2195
            NODE_19643_length_2237_cov_3.335014, whole genome shotgun sequence.
ACCESSION   DAOSUG010000502 DAOSUG010000000
VERSION     DAOSUG010000502.1
DBLINK      BioProject: PRJNA925477
            BioSample: SAMN32864262
            Sequence Read Archive: SRR4236673
KEYWORDS    WGS; ENV; Metagenome Assembled Genome; MAG; Third Party Data; TPA;
            TPA:assembly.
SOURCE      Micropruina sp. (wastewater metagenome)
  ORGANISM  Micropruina sp.
            Bacteria; Bacillati; Actinomycetota; Actinomycetes;
            Propionibacteriales; Nocardioidaceae; Micropruina.
REFERENCE   1  (bases 1 to 2237)
  AUTHORS   Abdulkadir,N., Saraiva,J.P., Zhang,J., Stolte,S., Gillor,O.,
            Harms,H. and Rocha,U.
  TITLE     Genome-centric analyses of 165 metagenomes show that mobile genetic
            elements are crucial for the transmission of antimicrobial
            resistance genes to pathogens in activated sludge and wastewater
  JOURNAL   Microbiol Spectr 12 (3), e0291823 (2024)
   PUBMED   38289113
REFERENCE   2  (bases 1 to 2237)
  AUTHORS   Abdulkadir,N.
  TITLE     Direct Submission
  JOURNAL   Submitted (24-MAR-2023) Environmental Microbiology, Helmholtz
            Center for Environmental Research -UFZ, Permoserstr 15, Leipzig
            04318, Germany
COMMENT     The annotation was added by the NCBI Prokaryotic Genome Annotation
            Pipeline (PGAP). Information about PGAP can be found here:
            https://www.ncbi.nlm.nih.gov/genome/annotation_prok/
            
            ##Genome-Assembly-Data-START##
            Assembly Method        :: SPAdes v. 3.11.1
            Genome Representation  :: Full
            Expected Final Version :: Yes
            Genome Coverage        :: Not Applicable
            Sequencing Technology  :: Illumina MiSeq
            ##Genome-Assembly-Data-END##
            
            ##Genome-Annotation-Data-START##
            Annotation Provider               :: NCBI
            Annotation Date                   :: 05/04/2023 04:57:10
            Annotation Pipeline               :: NCBI Prokaryotic Genome
                                                 Annotation Pipeline (PGAP)
            Annotation Method                 :: Best-placed reference protein
                                                 set; GeneMarkS-2+
            Annotation Software revision      :: 6.5
            Features Annotated                :: Gene; CDS; rRNA; tRNA; ncRNA
            Genes (total)                     :: 2,776
            CDSs (total)                      :: 2,750
            Genes (coding)                    :: 2,714
            CDSs (with protein)               :: 2,714
            Genes (RNA)                       :: 26
            tRNAs                             :: 24
            ncRNAs                            :: 2
            Pseudo Genes (total)              :: 36
            CDSs (without protein)            :: 36
            Pseudo Genes (ambiguous residues) :: 0 of 36
            Pseudo Genes (frameshifted)       :: 2 of 36
            Pseudo Genes (incomplete)         :: 32 of 36
            Pseudo Genes (internal stop)      :: 2 of 36
            ##Genome-Annotation-Data-END##
FEATURES             Location/Qualifiers
     source          1..2237
                     /organism="Micropruina sp."
                     /mol_type="genomic DNA"
                     /submitter_seqid="NODE_19643_length_2237_cov_3.335014"
                     /isolate="AMR_MDS_2195"
                     /isolation_source="Activated sludge of municipal
                     wastewater treatment plants"
                     /db_xref="taxon:2737536"
                     /environmental_sample
                     /geo_loc_name="Canada"
                     /lat_lon="49.25 N 123.1 W"
                     /collection_date="2015-06-16"
                     /metagenome_source="wastewater metagenome"
                     /note="metagenomic"
     gene            12..842
                     /gene="purU"
                     /locus_tag="PLF56_13325"
     CDS             12..842
                     /gene="purU"
                     /locus_tag="PLF56_13325"
                     /EC_number="3.5.1.10"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_014846185.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_function: GO:0008864 - formyltetrahydrofolate
                     deformylase activity [Evidence IEA];
                     GO_process: GO:0009152 - purine ribonucleotide
                     biosynthetic process [Evidence IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="formyltetrahydrofolate deformylase"
                     /protein_id="HOQ54579.1"
                     /translation="MLTLACDDRPGIVHAVTGAIAEAYGNITELQQFSSAESNRFFMR
                     VQVQSGFDRSVFEHAVSPIARSLGAQWVLDYVGRPRRTLILASKMGHCLNDLLFRARS
                     GNLQIEVPLILSNHGDLGGLAGFYGVTFEHHVVLPDTKAGFEARVLDAVQQHDIELVV
                     LARYMQILSPQLCEQLAGRAINIHHSFLPGFKGANPYRQAHSRGVKLIGATAHFVTAD
                     LDEGPIIEQNVRRVDHSVDVPELVQLGQEQESRTLSDAVKLFAEHRILLDGQRTVIFR
                     "
     gene            complement(1208..2038)
                     /locus_tag="PLF56_13330"
     CDS             complement(1208..2038)
                     /locus_tag="PLF56_13330"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_017934377.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="aldo/keto reductase"
                     /protein_id="HOQ54580.1"
                     /translation="MTAHTAHTGLTLPPIGFGTYRVRGDAGADTIARAIGNGYTLIDS
                     AFNYENEGAVGEGVRRSGRDRAELLVASKLPGRHHAYRRAVTTIEESVYRTGLGYLDL
                     YLIHWPNPLSDRYVEAWSAMIEARQQGLLRHIGVCNFLPEHLQRLQAETGVLPEVNQI
                     ELHPYFPQTELLAHDAAHGIITQAWSPLGRGNDLLAEPLVGRIAARYDATPGQVVLAW
                     AMARGAVPLPKAASDERQRENLAAVHLRLSESDIAAITSLGRPDGRIDDQDPAVYEEF
                     "
BASE COUNT          347 a          745 c          741 g          404 t
ORIGIN      
        1 ccacccactg gttgctgacc ctggcctgcg acgaccggcc cggcatcgtc catgcggtca
       61 cgggggcgat cgccgaggcc tacggcaaca tcaccgagct gcagcagttc agctcggcgg
      121 agtcgaaccg gttcttcatg cgcgtgcagg tgcagtcggg attcgaccgg tcggtgttcg
      181 agcacgcggt cagcccgatc gctcgttcgc tgggtgcgca atgggttctt gactacgtcg
      241 gacgcccccg gcgcaccttg atcctcgcct cgaagatggg gcactgcctc aacgatctgc
      301 tgtttcgcgc ccgctccggc aacctgcaga tcgaggtgcc gctgatcctc tcgaaccatg
      361 gcgacctggg tggtctcgca gggttctacg gggtgacgtt cgagcatcat gtggtgctgc
      421 ccgacaccaa ggccggcttc gaggcccggg tgctcgatgc cgttcagcag cacgacatcg
      481 aactggtggt gctggcccgc tacatgcaga ttctctcgcc gcagttgtgc gagcagctgg
      541 ccggccgggc gatcaacatt caccattcgt ttctgcccgg cttcaagggc gccaacccct
      601 accggcaggc gcacagccgt ggggtcaagc tgatcggcgc caccgcgcac ttcgtcaccg
      661 ccgacctcga cgaggggccg atcatcgaac agaacgtgcg ccgggtcgat cattcggtgg
      721 acgtgcccga gctcgtccag ctcggacagg agcaggagtc gcgcacactg agcgacgcgg
      781 tgaagctgtt cgccgagcac cgcattctgc tggacggcca gcgcaccgtc atcttccgct
      841 gacccagcgc accgaccgca tgcggacccg gcgtgctgat caatgcgcag cacactcgtc
      901 atctcaatgc gcagcacact cgtcatcccg ggcttgaccc gggatctcga cgcacgcaga
      961 gatcccggca tgcgccggga tgacagagtc accacatcga gggaacgctg agcaatgcgc
     1021 agcacacttc atcccgggct gccccgggat cccgccgcac acagagatcc cggctgttcg
     1081 ctcgggatga cgggaatcac gacttcagtg acgctttgat caacctgcac gatccgttgg
     1141 ttgagctgga cgaacccctc tcacgatggc tcgccaggct cgaccagcct ttccttcgtc
     1201 ggcccggtca gaactcctcg taaacggcgg ggtcctggtc gtcgatgcgt ccatccggac
     1261 gtccgaggga cgtgatcgcg gcgatgtccg attcgctgag ccgcaggtgg acggccgcca
     1321 ggttctcgcg ctggcgctcg tccgaggccg ccttcggcag ggggacggcc ccgcgcgcca
     1381 tcgcccaggc gagcaccacc tgcccggggg tggcgtcgta cctggccgcg atgcggccca
     1441 ccagcggctc ggcgagcaga tcgttgccgc ggcccagcgg gctccacgcc tgcgtgatga
     1501 tgccgtgcgc cgcgtcgtgg gcgagcagct cggtctgtgg aaagtacggg tgcagttcga
     1561 tctggttgac ctcgggcaat actccggtct cggcctgcag ccgctgcagg tgctcgggta
     1621 gaaagttgca gaccccgatg tgtcgcagca gaccctgctg ccgcgcctcg atcatggccg
     1681 accaggcttc gacgtatcgg tcgctgagcg ggttcggcca atggatcagg tacaggtcga
     1741 gataacccaa cccggtgcgg tagacgcttt cttcgatggt ggtcacggcc cttcggtagg
     1801 cgtggtgccg tccgggcagc ttgctggcga ccagtagctc ggcacggtcg cgccccgagc
     1861 gtcggacgcc ctcaccgacc gcaccctcgt tctcatagtt gaacgcggaa tcgatcagcg
     1921 tgtacccgtt gccgatagct cgggcgatgg tgtcggcgcc ggcgtcgccg cgcacccggt
     1981 aggtgccaaa acctatcggc ggcagcgtca ggccggtgtg ggcggtgtgg gcggtcatgg
     2041 ctgactcctt atttctgcgg tgtggttctg cggtcacgtc gggcgtcccc tccgtccagg
     2101 ctcacccaca ggcaggcccc ggcacgaggt gtcgcccgtc agcgggtagg ggtgtagatg
     2161 ctctcggcgt agcacgagcc gtagtaggtc tcgagttgct ccagggtctc gggggtgttc
     2221 ggaagcacgg ccttggt
//
LOCUS       DAOSUG010000503         2229 bp    DNA     linear   ENV 10-JUN-2024
DEFINITION  MAG TPA_asm: Micropruina sp. isolate AMR_MDS_2195
            NODE_19736_length_2229_cov_4.970561, whole genome shotgun sequence.
ACCESSION   DAOSUG010000503 DAOSUG010000000
VERSION     DAOSUG010000503.1
DBLINK      BioProject: PRJNA925477
            BioSample: SAMN32864262
            Sequence Read Archive: SRR4236673
KEYWORDS    WGS; ENV; Metagenome Assembled Genome; MAG; Third Party Data; TPA;
            TPA:assembly.
SOURCE      Micropruina sp. (wastewater metagenome)
  ORGANISM  Micropruina sp.
            Bacteria; Bacillati; Actinomycetota; Actinomycetes;
            Propionibacteriales; Nocardioidaceae; Micropruina.
REFERENCE   1  (bases 1 to 2229)
  AUTHORS   Abdulkadir,N., Saraiva,J.P., Zhang,J., Stolte,S., Gillor,O.,
            Harms,H. and Rocha,U.
  TITLE     Genome-centric analyses of 165 metagenomes show that mobile genetic
            elements are crucial for the transmission of antimicrobial
            resistance genes to pathogens in activated sludge and wastewater
  JOURNAL   Microbiol Spectr 12 (3), e0291823 (2024)
   PUBMED   38289113
REFERENCE   2  (bases 1 to 2229)
  AUTHORS   Abdulkadir,N.
  TITLE     Direct Submission
  JOURNAL   Submitted (24-MAR-2023) Environmental Microbiology, Helmholtz
            Center for Environmental Research -UFZ, Permoserstr 15, Leipzig
            04318, Germany
COMMENT     The annotation was added by the NCBI Prokaryotic Genome Annotation
            Pipeline (PGAP). Information about PGAP can be found here:
            https://www.ncbi.nlm.nih.gov/genome/annotation_prok/
            
            ##Genome-Assembly-Data-START##
            Assembly Method        :: SPAdes v. 3.11.1
            Genome Representation  :: Full
            Expected Final Version :: Yes
            Genome Coverage        :: Not Applicable
            Sequencing Technology  :: Illumina MiSeq
            ##Genome-Assembly-Data-END##
            
            ##Genome-Annotation-Data-START##
            Annotation Provider               :: NCBI
            Annotation Date                   :: 05/04/2023 04:57:10
            Annotation Pipeline               :: NCBI Prokaryotic Genome
                                                 Annotation Pipeline (PGAP)
            Annotation Method                 :: Best-placed reference protein
                                                 set; GeneMarkS-2+
            Annotation Software revision      :: 6.5
            Features Annotated                :: Gene; CDS; rRNA; tRNA; ncRNA
            Genes (total)                     :: 2,776
            CDSs (total)                      :: 2,750
            Genes (coding)                    :: 2,714
            CDSs (with protein)               :: 2,714
            Genes (RNA)                       :: 26
            tRNAs                             :: 24
            ncRNAs                            :: 2
            Pseudo Genes (total)              :: 36
            CDSs (without protein)            :: 36
            Pseudo Genes (ambiguous residues) :: 0 of 36
            Pseudo Genes (frameshifted)       :: 2 of 36
            Pseudo Genes (incomplete)         :: 32 of 36
            Pseudo Genes (internal stop)      :: 2 of 36
            ##Genome-Annotation-Data-END##
FEATURES             Location/Qualifiers
     source          1..2229
                     /organism="Micropruina sp."
                     /mol_type="genomic DNA"
                     /submitter_seqid="NODE_19736_length_2229_cov_4.970561"
                     /isolate="AMR_MDS_2195"
                     /isolation_source="Activated sludge of municipal
                     wastewater treatment plants"
                     /db_xref="taxon:2737536"
                     /environmental_sample
                     /geo_loc_name="Canada"
                     /lat_lon="49.25 N 123.1 W"
                     /collection_date="2015-06-16"
                     /metagenome_source="wastewater metagenome"
                     /note="metagenomic"
     gene            <1..1321
                     /gene="glmS"
                     /locus_tag="PLF56_13335"
     CDS             <1..1321
                     /gene="glmS"
                     /locus_tag="PLF56_13335"
                     /EC_number="2.6.1.16"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_004808965.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_function: GO:0004360 - glutamine-fructose-6-phosphate
                     transaminase (isomerizing) activity [Evidence IEA];
                     GO_process: GO:0006040 - amino sugar metabolic process
                     [Evidence IEA];
                     GO_process: GO:0009252 - peptidoglycan biosynthetic
                     process [Evidence IEA]"
                     /codon_start=2
                     /transl_table=11
                     /product="glutamine--fructose-6-phosphate transaminase
                     (isomerizing)"
                     /protein_id="HOQ54581.1"
                     /translation="RRNSPLVVGLGEGENFVASDVSAFIEFTRHAVELGQDRVVDVTA
                     DSFSVTDFDGNPADSHPFEVTWDLSAAEKQGYDWFMRKEIYEQPQAVADTLLGRYNED
                     GSLKLDELHIPEAELRTIDKIIIVACGTAYYAGMVAKYAIEHWTRIACEVEIASEFRY
                     RDPIVTPLTLVVTLSQSGETADTLMAIRHAREQGAKVIAICNTNGATIPRESDAVIYT
                     HAGPEIGVASTKGFLTQVAACYLLGLYLAQVRGTKYGDEIAAIMSELAATPPLIQRVL
                     DEIEPIKQLAAKYAEQRSVLFLGRHVGYPVALEGALKLKEIAYLHAEGFPAGELKHGP
                     IALVENQIPIFVVVPPQGRDQLHDKVTSNIQEVRARGAFTIVLAEDDDDEVTPYADFI
                     FRLPKVSTLMQPLVATVPLQMFACELATLKGYDVDQPRNLAKSVTVE"
     gene            1318..1665
                     /locus_tag="PLF56_13340"
     CDS             1318..1665
                     /locus_tag="PLF56_13340"
                     /EC_number="2.7.8.7"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_011754369.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_function: GO:0008897 - holo-[acyl-carrier-protein]
                     synthase activity [Evidence IEA];
                     GO_process: GO:0006633 - fatty acid biosynthetic process
                     [Evidence IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="holo-ACP synthase"
                     /protein_id="HOQ54582.1"
                     /translation="MIIGIGIDVCPVERFARAAERQRLLDRLFTAAEQDTSMNRMAGR
                     FAAKEALAKALGAPEGLGWHDVEVRKTPQGKPYFAYTASVAERIASLGIGTVHLSITH
                     DAGIAAAVVVCEA"
     gene            1670..>2229
                     /locus_tag="PLF56_13345"
     CDS             1670..>2229
                     /locus_tag="PLF56_13345"
                     /inference="COORDINATES: protein motif:HMM:NF015791.2"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="NAD(P)H-hydrate epimerase"
                     /protein_id="HOQ54583.1"
                     /translation="MRFAYLAETIRAAEAPLLEAQPGLLMQRAATGLASVLLRELRTL
                     RGGAYGARVLIVAGSGNNGGDALFAGAQLARRGVRVRAWLTSERAHEQGWAAFIGAGG
                     RQLDALGALAELGSSDLVVDGVLGIGGRAGLREPVALFAKACADAGVPVVAVDLPSGL
                     DADSCAAEIPASAGMTGSPRDPGQLG"
BASE COUNT          351 a          780 c          761 g          337 t
ORIGIN      
        1 gcggcgcaac tcgccgctgg tggtcggcct cggtgagggt gagaacttcg tcgcctcgga
       61 cgtctcggcg ttcatcgagt tcacccggca cgccgtcgag ctcggccaag accgggtggt
      121 cgacgtcacc gccgacagct tcagcgtcac cgacttcgac ggcaaccccg ccgacagcca
      181 cccgttcgag gtcacctggg acctttccgc ggctgagaag cagggctacg actggttcat
      241 gcgcaaagag atctacgagc agccgcaggc ggtggccgac accctgctcg gccgctacaa
      301 cgaggacggc tcgctcaagc tcgacgaact gcacattccc gaagccgagt tgcgcaccat
      361 cgacaagatc atcatcgtgg cttgtggcac cgcctattac gcgggaatgg tcgccaagta
      421 cgcgatcgag cactggacgc ggatcgcctg cgaggtcgag atcgcctccg agttccgcta
      481 ccgcgacccg atcgtcaccc cgctcaccct ggtggtgacg ctcagccagt cgggtgagac
      541 cgccgacacg ctgatggcga tccggcacgc ccgcgagcag ggcgccaagg tgatcgccat
      601 ctgcaacacc aacggcgcca ccattccgcg cgagtcggac gcggtcatct acacccacgc
      661 cggccccgag atcggcgtcg cgtccaccaa gggctttctc acccaggtgg ccgcctgcta
      721 cttgctcggt ctgtacctcg cccaggtgcg cggcaccaag tacggcgacg agatcgcggc
      781 catcatgagc gagctcgccg ccacgccgcc gctgatccag cgcgtcctgg acgagatcga
      841 gccgatcaag cagctcgccg ccaaatacgc cgagcagcgg tcggtgctgt tcctgggccg
      901 gcacgtcggg tacccggtgg ccctggaggg cgcgctgaag ctgaaggaga tcgcctacct
      961 gcacgccgaa ggctttccgg ccggcgagct caagcacggt ccgatcgccc tggtcgagaa
     1021 ccagatcccg atcttcgtgg tggtgccgcc gcagggccgc gaccagctgc acgacaaggt
     1081 gacctcgaac attcaagagg tgcgggcccg gggggcgttc accatcgtgc tggccgagga
     1141 cgacgacgac gaggtcaccc cgtacgccga cttcatcttc agactgccca aggtgtcgac
     1201 gctgatgcag ccgctggtgg ccaccgtgcc gctgcagatg ttcgcctgcg aactggccac
     1261 cctgaagggc tacgacgtcg accagccacg caacctggcc aagtcggtca cggtcgagtg
     1321 atcatcggca tcggcatcga cgtctgcccg gtcgaacggt tcgctcgggc cgccgaacgg
     1381 cagcgtctgc tggaccgctt gttcaccgcg gccgaacagg acacctcgat gaaccgcatg
     1441 gccggacgct tcgccgccaa agaggccctg gccaaggcgc tcggtgcccc cgagggactc
     1501 ggttggcacg atgtcgaggt gcgcaagacc cctcagggca agccctattt cgcctacacc
     1561 gcctcggtcg ccgagcgcat cgcgtcgctc ggcatcggca ccgtgcacct gtcgatcacc
     1621 cacgacgcgg gcatcgccgc cgcggtggtg gtctgcgagg cgtgaggcga tgaggttcgc
     1681 gtacctggcc gagacgatcc gggccgccga ggcgccgctg ctcgaggcgc aacccgggct
     1741 gttgatgcag cgggccgcga ccgggctcgc gtcggtgctg ctgcgcgagc ttcggacgct
     1801 gcgcgggggc gcgtacggcg cccgggtgct gatcgtcgcc ggctccggca acaacggtgg
     1861 cgacgcgctg ttcgccgggg cccagttggc ccgtcgcggg gtgcgggtgc gggcctggct
     1921 cacctctgaa cgcgcgcatg agcaggggtg ggcggccttc atcggcgccg gtggacgcca
     1981 gctggacgcg ctcggcgccc tggccgagct gggatcgtcc gatctcgtgg tggacggcgt
     2041 gctcggcatc ggcggacgcg ccggcctgcg tgaaccggtc gccctgttcg ccaaagcctg
     2101 cgcggacgcc ggcgtgccgg tggtggcggt cgacctgccg tccggattgg acgccgactc
     2161 gtgcgcggca gagatcccgg cgtccgccgg gatgacggga agcccgcgtg atcccggcca
     2221 actcggtca
//
LOCUS       DAOSUG010000504         2228 bp    DNA     linear   ENV 10-JUN-2024
DEFINITION  MAG TPA_asm: Micropruina sp. isolate AMR_MDS_2195
            NODE_19752_length_2228_cov_3.104924, whole genome shotgun sequence.
ACCESSION   DAOSUG010000504 DAOSUG010000000
VERSION     DAOSUG010000504.1
DBLINK      BioProject: PRJNA925477
            BioSample: SAMN32864262
            Sequence Read Archive: SRR4236673
KEYWORDS    WGS; ENV; Metagenome Assembled Genome; MAG; Third Party Data; TPA;
            TPA:assembly.
SOURCE      Micropruina sp. (wastewater metagenome)
  ORGANISM  Micropruina sp.
            Bacteria; Bacillati; Actinomycetota; Actinomycetes;
            Propionibacteriales; Nocardioidaceae; Micropruina.
REFERENCE   1  (bases 1 to 2228)
  AUTHORS   Abdulkadir,N., Saraiva,J.P., Zhang,J., Stolte,S., Gillor,O.,
            Harms,H. and Rocha,U.
  TITLE     Genome-centric analyses of 165 metagenomes show that mobile genetic
            elements are crucial for the transmission of antimicrobial
            resistance genes to pathogens in activated sludge and wastewater
  JOURNAL   Microbiol Spectr 12 (3), e0291823 (2024)
   PUBMED   38289113
REFERENCE   2  (bases 1 to 2228)
  AUTHORS   Abdulkadir,N.
  TITLE     Direct Submission
  JOURNAL   Submitted (24-MAR-2023) Environmental Microbiology, Helmholtz
            Center for Environmental Research -UFZ, Permoserstr 15, Leipzig
            04318, Germany
COMMENT     The annotation was added by the NCBI Prokaryotic Genome Annotation
            Pipeline (PGAP). Information about PGAP can be found here:
            https://www.ncbi.nlm.nih.gov/genome/annotation_prok/
            
            ##Genome-Assembly-Data-START##
            Assembly Method        :: SPAdes v. 3.11.1
            Genome Representation  :: Full
            Expected Final Version :: Yes
            Genome Coverage        :: Not Applicable
            Sequencing Technology  :: Illumina MiSeq
            ##Genome-Assembly-Data-END##
            
            ##Genome-Annotation-Data-START##
            Annotation Provider               :: NCBI
            Annotation Date                   :: 05/04/2023 04:57:10
            Annotation Pipeline               :: NCBI Prokaryotic Genome
                                                 Annotation Pipeline (PGAP)
            Annotation Method                 :: Best-placed reference protein
                                                 set; GeneMarkS-2+
            Annotation Software revision      :: 6.5
            Features Annotated                :: Gene; CDS; rRNA; tRNA; ncRNA
            Genes (total)                     :: 2,776
            CDSs (total)                      :: 2,750
            Genes (coding)                    :: 2,714
            CDSs (with protein)               :: 2,714
            Genes (RNA)                       :: 26
            tRNAs                             :: 24
            ncRNAs                            :: 2
            Pseudo Genes (total)              :: 36
            CDSs (without protein)            :: 36
            Pseudo Genes (ambiguous residues) :: 0 of 36
            Pseudo Genes (frameshifted)       :: 2 of 36
            Pseudo Genes (incomplete)         :: 32 of 36
            Pseudo Genes (internal stop)      :: 2 of 36
            ##Genome-Annotation-Data-END##
FEATURES             Location/Qualifiers
     source          1..2228
                     /organism="Micropruina sp."
                     /mol_type="genomic DNA"
                     /submitter_seqid="NODE_19752_length_2228_cov_3.104924"
                     /isolate="AMR_MDS_2195"
                     /isolation_source="Activated sludge of municipal
                     wastewater treatment plants"
                     /db_xref="taxon:2737536"
                     /environmental_sample
                     /geo_loc_name="Canada"
                     /lat_lon="49.25 N 123.1 W"
                     /collection_date="2015-06-16"
                     /metagenome_source="wastewater metagenome"
                     /note="metagenomic"
     gene            <1..204
                     /locus_tag="PLF56_13350"
     CDS             <1..204
                     /locus_tag="PLF56_13350"
                     /inference="COORDINATES: ab initio
                     prediction:GeneMarkS-2+"
                     /note="Derived by automated computational analysis using
                     gene prediction method: GeneMarkS-2+."
                     /codon_start=1
                     /transl_table=11
                     /product="hypothetical protein"
                     /protein_id="HOQ54584.1"
                     /translation="AELAAYYEHKGVSPSTARLVAQELTEADSLTAQLEIEGFDERTT
                     VASAIAGALWAGGHFCSARRCRC"
     gene            210..395
                     /locus_tag="PLF56_13355"
     CDS             210..395
                     /locus_tag="PLF56_13355"
                     /inference="COORDINATES: protein motif:HMM:NF014090.2"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="hypothetical protein"
                     /protein_id="HOQ54585.1"
                     /translation="MAAPGSWRDEYTLAAVAVALAITSVVLARMGHTRIWQTLLRSVF
                     IGLAALGSSYLIGSALL"
     gene            650..1570
                     /locus_tag="PLF56_13360"
     CDS             650..1570
                     /locus_tag="PLF56_13360"
                     /EC_number="1.-.-.-"
                     /inference="COORDINATES: protein motif:HMM:NF024989.2"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_function: GO:0016491 - oxidoreductase activity
                     [Evidence IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="NADP-dependent oxidoreductase"
                     /protein_id="HOQ54586.1"
                     /translation="MRAVTYDQFGDDSVLAVTQQPLPKVAPGEVLVRVRAAGVNPVDW
                     KVMSGGLDALMETQFPVVPGWDVAGVIEQVGIDVTDYAVGDEVIGYARKDWVHAGTFA
                     EFVAVPRRCLAHKPARLSWHQAAGLPLAGLTAYQVLHRLGLAQGRTVLIHGGAGGVGS
                     IGVQLAAAAGARVIATASEANHDYLRELGAEPVTYGDGLADRVRALAPDGVDIVADFV
                     GGVVDATVSVLAKGGVHASIADPAVLKAGGQWMWVHPDAADLAALADLVQSGAVNVEV
                     AATFGLDEVPAAFALSREGHTRGKIVIDVA"
BASE COUNT          302 a          724 c          827 g          375 t
ORIGIN      
        1 gccgagctcg ccgcatacta cgaacacaag ggcgtgagtc cgtcgaccgc ccgcctggtg
       61 gcccaggaac tcacggaggc cgacagcctc accgcacaac tcgagatcga gggcttcgac
      121 gagcgcacca cggtggcatc cgccatcgcc ggcgcgctct gggcgggtgg gcatttctgc
      181 tcggcgcggc ggtgccggtg ctgatctcgg tggctgcgcc cggaagctgg cgggacgagt
      241 acaccttggc ggcggtggcg gtggcactgg cgatcacgtc cgtggtgctg gcccggatgg
      301 ggcatacccg gatctggcag accctgttgc gctcggtgtt catcggcctg gcggccctcg
      361 gttcgagcta cctgatcggt tcggcgttgc tgtgacgaac gggcgagcgc gagtgacgct
      421 tgctcgggtc cgctggagcg tgaccccatc cggggtggcg ggggtggcca ctgacgcgcg
      481 ctcgggttcc tgagcgtgac cctgccggcg gtgtcaccgg ggccaccacc ggtgacgggc
      541 aactgcgctg tgcccccagc cgcgaccgca ggcgccgggc acgcggaacc gcggcgtggt
      601 gaacgggaat cgtccgtgtt gtctgcgggt tgcatgaggc atgacgagca tgcgcgcagt
      661 gacctatgac cagttcggcg acgattcggt gttggcggtg acccagcaac ccctgccgaa
      721 ggtggcaccg ggcgaggtgc tggtacgggt cagggccgcg ggggtgaacc cggtcgactg
      781 gaaggtcatg tccggcggac tggacgccct gatggagacc cagttcccgg tcgtccccgg
      841 gtgggacgtc gcgggcgtga tcgagcaggt cggcatcgac gtcacggact atgcggtcgg
      901 cgatgaggtg atcggctacg cccgcaagga ctgggtgcat gccggcacgt tcgccgagtt
      961 cgtcgctgtt ccccggcgtt gcttggcgca caagccggcg cggttgagct ggcaccaggc
     1021 ggccgggctg ccgctcgccg ggctgaccgc ctaccaggtg ctgcaccggc tgggcctggc
     1081 gcagggccgc accgtgctga ttcacggtgg tgcgggtggt gtcggctcga tcggcgtcca
     1141 gctcgcggct gcggcgggcg cgcgggtgat cgcgacggcg tccgaggcga atcacgacta
     1201 tctgcgcgag ttgggcgccg agccggtgac ctatggcgac ggcctcgccg accgggtccg
     1261 cgcactggca ccggacggcg tcgacatcgt ggccgacttc gttggcggcg tggtggacgc
     1321 caccgtgtcc gtgctcgcga agggcggtgt gcatgcgtcc atcgccgatc cggccgtgct
     1381 gaaagcgggt ggccagtgga tgtgggtgca ccccgacgcg gccgacctcg cggcgctggc
     1441 cgacctcgtc caatcgggtg cggtgaatgt cgaagtggct gccaccttcg gcctggacga
     1501 ggtgcccgcc gccttcgcgc tcagccgcga gggccacacc cggggcaaaa tcgtcatcga
     1561 cgtggcctga gcggtacaac cgcggcgctg ccggcgcact gcgggcgtgg atgcgctgac
     1621 cggctggcgg cgacgctgtt catcggcctc gcggccatgg gtttcagcta tctggtgggg
     1681 tcggtactgc tgtagcgcgc atcgtccggc gcgtcaggca cgcacggctg gaggacctgc
     1741 tcagtcgccg gccgaccacg tggccgcggt ggtcctgcgc gctgccccgg tcagtggcgg
     1801 tcgcagagcg gccagccgtt ccagaccggc ctccagcacg cttggctcgt gccagatcgg
     1861 aactcggatc atcgtttgct gtccatcgtt ggccgagaag gtgggcccgg cgaccatcca
     1921 gatacccgca cgtcgggcat gttcggccag cgcggtcgcc tcgacgccgg tgtcgaagca
     1981 ctcagcgccc gcgggagctc gaacgtgacg atcgaagcgc tccggccttg atgccttaat
     2041 gccccaccgt ctttgaacag acactcatca ccacgcccac cgaagcgctc agccggatcc
     2101 actggcgtgc aggccgatcg ccggcctgcg ttcttgacga gtttcttgcg gccaagccgt
     2161 gtgctcattt gggtctgttc cttggaacgg gcagaagtcg cggggttaca gcgcgttctg
     2221 cagaatgg
//
LOCUS       DAOSUG010000505         2213 bp    DNA     linear   ENV 10-JUN-2024
DEFINITION  MAG TPA_asm: Micropruina sp. isolate AMR_MDS_2195
            NODE_19947_length_2213_cov_3.371640, whole genome shotgun sequence.
ACCESSION   DAOSUG010000505 DAOSUG010000000
VERSION     DAOSUG010000505.1
DBLINK      BioProject: PRJNA925477
            BioSample: SAMN32864262
            Sequence Read Archive: SRR4236673
KEYWORDS    WGS; ENV; Metagenome Assembled Genome; MAG; Third Party Data; TPA;
            TPA:assembly.
SOURCE      Micropruina sp. (wastewater metagenome)
  ORGANISM  Micropruina sp.
            Bacteria; Bacillati; Actinomycetota; Actinomycetes;
            Propionibacteriales; Nocardioidaceae; Micropruina.
REFERENCE   1  (bases 1 to 2213)
  AUTHORS   Abdulkadir,N., Saraiva,J.P., Zhang,J., Stolte,S., Gillor,O.,
            Harms,H. and Rocha,U.
  TITLE     Genome-centric analyses of 165 metagenomes show that mobile genetic
            elements are crucial for the transmission of antimicrobial
            resistance genes to pathogens in activated sludge and wastewater
  JOURNAL   Microbiol Spectr 12 (3), e0291823 (2024)
   PUBMED   38289113
REFERENCE   2  (bases 1 to 2213)
  AUTHORS   Abdulkadir,N.
  TITLE     Direct Submission
  JOURNAL   Submitted (24-MAR-2023) Environmental Microbiology, Helmholtz
            Center for Environmental Research -UFZ, Permoserstr 15, Leipzig
            04318, Germany
COMMENT     The annotation was added by the NCBI Prokaryotic Genome Annotation
            Pipeline (PGAP). Information about PGAP can be found here:
            https://www.ncbi.nlm.nih.gov/genome/annotation_prok/
            
            ##Genome-Assembly-Data-START##
            Assembly Method        :: SPAdes v. 3.11.1
            Genome Representation  :: Full
            Expected Final Version :: Yes
            Genome Coverage        :: Not Applicable
            Sequencing Technology  :: Illumina MiSeq
            ##Genome-Assembly-Data-END##
            
            ##Genome-Annotation-Data-START##
            Annotation Provider               :: NCBI
            Annotation Date                   :: 05/04/2023 04:57:10
            Annotation Pipeline               :: NCBI Prokaryotic Genome
                                                 Annotation Pipeline (PGAP)
            Annotation Method                 :: Best-placed reference protein
                                                 set; GeneMarkS-2+
            Annotation Software revision      :: 6.5
            Features Annotated                :: Gene; CDS; rRNA; tRNA; ncRNA
            Genes (total)                     :: 2,776
            CDSs (total)                      :: 2,750
            Genes (coding)                    :: 2,714
            CDSs (with protein)               :: 2,714
            Genes (RNA)                       :: 26
            tRNAs                             :: 24
            ncRNAs                            :: 2
            Pseudo Genes (total)              :: 36
            CDSs (without protein)            :: 36
            Pseudo Genes (ambiguous residues) :: 0 of 36
            Pseudo Genes (frameshifted)       :: 2 of 36
            Pseudo Genes (incomplete)         :: 32 of 36
            Pseudo Genes (internal stop)      :: 2 of 36
            ##Genome-Annotation-Data-END##
FEATURES             Location/Qualifiers
     source          1..2213
                     /organism="Micropruina sp."
                     /mol_type="genomic DNA"
                     /submitter_seqid="NODE_19947_length_2213_cov_3.371640"
                     /isolate="AMR_MDS_2195"
                     /isolation_source="Activated sludge of municipal
                     wastewater treatment plants"
                     /db_xref="taxon:2737536"
                     /environmental_sample
                     /geo_loc_name="Canada"
                     /lat_lon="49.25 N 123.1 W"
                     /collection_date="2015-06-16"
                     /metagenome_source="wastewater metagenome"
                     /note="metagenomic"
     gene            complement(<1..354)
                     /locus_tag="PLF56_13365"
     CDS             complement(<1..354)
                     /locus_tag="PLF56_13365"
                     /inference="COORDINATES: protein motif:HMM:NF018446.2"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_function: GO:0022841 - potassium ion leak channel
                     activity [Evidence IEA];
                     GO_process: GO:0071805 - potassium ion transmembrane
                     transport [Evidence IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="TMEM175 family protein"
                     /protein_id="HOQ54587.1"
                     /translation="MTGNTPAASNRPRVPPAERLVFFTDAVVAIAMTLLILPLMESIG
                     DAPDEGDTLHWLGAHRSQLVSFVLSFVIISVFWRAHHRLFEHVATVTQGLFVLDFCWM
                     FTVVWLPIATALTGAM"
     gene            461..790
                     /locus_tag="PLF56_13370"
     CDS             461..790
                     /locus_tag="PLF56_13370"
                     /EC_number="1.-.-.-"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_013161229.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_function: GO:0004497 - monooxygenase activity [Evidence
                     IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="putative quinol monooxygenase"
                     /protein_id="HOQ54588.1"
                     /translation="MITITVKWDVKPEYADDFMELTREFTEGTRAEPGCLWFDWSRSV
                     DNPNEYILIEAFRDGEAGGQHVNSDHFAKAMATQGQYAASRPKIISYEIDQDGWNELG
                     EIQMPGE"
     gene            complement(812..1177)
                     /locus_tag="PLF56_13375"
     CDS             complement(812..1177)
                     /locus_tag="PLF56_13375"
                     /inference="COORDINATES: ab initio
                     prediction:GeneMarkS-2+"
                     /note="Derived by automated computational analysis using
                     gene prediction method: GeneMarkS-2+."
                     /codon_start=1
                     /transl_table=11
                     /product="hypothetical protein"
                     /protein_id="HOQ54589.1"
                     /translation="MGAETPQQYLDAAPEPGRPWLEEFWAHVQQHCPDLPLVMFRSTP
                     MFKFTDSYLKGYVMFTAAKGHFSAHAIDFDLVAAAQQSIKGAFGDKGSVSVKYANTDA
                     KPQLIDFVDDVLRRHGYPG"
     gene            1278..2162
                     /locus_tag="PLF56_13380"
     CDS             1278..2162
                     /locus_tag="PLF56_13380"
                     /EC_number="1.-.-.-"
                     /inference="COORDINATES: protein motif:HMM:NF012334.2"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_function: GO:0016491 - oxidoreductase activity
                     [Evidence IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="SDR family oxidoreductase"
                     /protein_id="HOQ54590.1"
                     /translation="MFFTRSSDPVDLSGRTAVVTGGGTGLGAAITALLAGRGCRVLIG
                     SRNEERVTKAAAQWRADGLAVDARRCDVTSREDVFALRDAALTSTGRLDLWVNNAGRA
                     GAFGPIRLTPESDFLATTDTVIRGTYFGSLAALDVMVPQGAGDLINLLGRGDDAPVTN
                     QAAYGSAKTWVRAFTRALASEQKGSGVRVHAFNPGLVRTDMLGRIDAVRGYGQGLARF
                     PSVVATLGLDPDEAARPLLDLIGSDRLEYRGTEPVRLLGAATGNLRRRMKHGRSAPEP
                     IEVTEVEPRAVDQPPTPA"
BASE COUNT          373 a          736 c          771 g          333 t
ORIGIN      
        1 catcgcgccg gtcagcgccg tggctatcgg cagccagacc acggtgaaca tccagcagaa
       61 gtcgagtacg aacagcccct gcgtgaccgt ggcgacgtgt tcgaacaggc gatggtgcgc
      121 gcgccagaac accgagatga tcacgaagct cagaacgaaa ctgaccagct gactgcgatg
      181 cgcacccagc cagtgcaggg tgtcgccctc gtcgggggca tcgccgatgc tctccatcaa
      241 gggcaggatc aacagcgtca tcgcgatcgc caccaccgcg tcggtgaaga acaccaggcg
      301 ttcggcgggg ggcacccgtg gtcggttgga ggcggcgggg gtgttgccgg tcatggtgag
      361 cagcgtaggc cagcccgggc gatgcgcgcg cggcaccaca ttgtcgcggg gtgagaatgt
      421 ggggctgccc agcgcctcga atcttgtgca gactatcccc atgatcacca tcacagtgaa
      481 gtgggatgtg aagcccgagt acgccgacga cttcatggag ttgacccgcg agttcaccga
      541 gggcacccgc gccgagccgg gctgcctgtg gttcgactgg tcgcgcagcg tcgacaaccc
      601 gaacgagtac atcctgatcg aggcgttccg cgacggtgag gccggcgggc agcacgtgaa
      661 cagcgatcac ttcgccaagg cgatggccac ccagggccag tacgcggcca gccgtccgaa
      721 gatcatcagc tacgagatcg accaggacgg ctggaacgag ctcggcgaaa tccagatgcc
      781 gggcgagtga gagctgagcc tggatccagg ctcagccggg gtagccgtgg cggcgtagca
      841 cgtcgtccac gaaatcaatg agttgcggct tggcgtcggt gttggcgtac ttcacgctca
      901 ccgacccctt gtcgccgaac gcgcccttga tcgactgttg cgcggccgcg accaggtcga
      961 agtcgattgc gtgcgccgag aaatgcccct tggctgcggt gaacatcacg tagcccttga
     1021 ggtagctgtc ggtgaacttg aacatcggcg tcgaccggaa catcaccagc ggcaggtccg
     1081 gacaatgctg ttgcacgtgg gcccagaact cctccagcca cggacgtccg ggctcggggg
     1141 cggcgtccag atactgctgc ggcgtctcgg cacccatcac aacccccggc gccgcgggtc
     1201 tgctgcgatc atggggtcac attggcacgc ccggcgcagg tcggcaaggt gcgtgcccct
     1261 gaccagccgg aggtgtcatg ttcttcacgc gcagttctga tcccgtcgac ctgtccggac
     1321 ggacggcggt ggtcaccggc ggcggcaccg gtctgggtgc ggccatcacc gccctactgg
     1381 ccggacgagg ctgccgggtg ctgatcggct cgcgcaacga agaacgggtg acgaaggccg
     1441 ccgcgcagtg gcgcgcggac ggtctggccg tggacgcgcg gcgctgcgac gtcaccagtc
     1501 gcgaggacgt gttcgcgctg cgcgacgcgg ccctgaccag caccggacgc ctcgacctgt
     1561 gggtcaacaa cgccggccgg gcgggcgcct tcggcccgat ccggctgacc cccgagagcg
     1621 actttctggc caccaccgac accgtgatcc ggggcaccta cttcggctcg ctggccgccc
     1681 tcgacgtgat ggtcccccag ggtgccggcg acctgatcaa cctgctcgga cgcggtgacg
     1741 acgcccccgt caccaaccag gcggcctacg gatcggcgaa gacgtgggtg cgggccttca
     1801 cccgggcgct ggcgtccgag cagaagggca gcggcgtgcg ggtgcatgcc ttcaatcccg
     1861 gactggtgcg caccgacatg ctgggccgca tcgacgccgt ccgggggtat gggcagggac
     1921 tggcgcggtt cccgtccgtc gtggcgactc tggggctgga tcccgacgag gcagcgcggc
     1981 cgctgctcga cctgatcggc agcgaccgcc tggagtaccg cggcaccgaa ccggttcgcc
     2041 tgctcggcgc cgcgacaggc aacctgcgtc gccggatgaa gcacggccgc tccgcgccgg
     2101 agcccatcga ggtgaccgag gtggagcccc gtgcggtcga tcaaccccct acgcccgcct
     2161 aagtatcgtg cgcctgcgaa gcttcgcggg cgcacgatac ttgggcgggc gtt
//
LOCUS       DAOSUG010000506         2190 bp    DNA     linear   ENV 10-JUN-2024
DEFINITION  MAG TPA_asm: Micropruina sp. isolate AMR_MDS_2195
            NODE_20229_length_2190_cov_3.969555, whole genome shotgun sequence.
ACCESSION   DAOSUG010000506 DAOSUG010000000
VERSION     DAOSUG010000506.1
DBLINK      BioProject: PRJNA925477
            BioSample: SAMN32864262
            Sequence Read Archive: SRR4236673
KEYWORDS    WGS; ENV; Metagenome Assembled Genome; MAG; Third Party Data; TPA;
            TPA:assembly.
SOURCE      Micropruina sp. (wastewater metagenome)
  ORGANISM  Micropruina sp.
            Bacteria; Bacillati; Actinomycetota; Actinomycetes;
            Propionibacteriales; Nocardioidaceae; Micropruina.
REFERENCE   1  (bases 1 to 2190)
  AUTHORS   Abdulkadir,N., Saraiva,J.P., Zhang,J., Stolte,S., Gillor,O.,
            Harms,H. and Rocha,U.
  TITLE     Genome-centric analyses of 165 metagenomes show that mobile genetic
            elements are crucial for the transmission of antimicrobial
            resistance genes to pathogens in activated sludge and wastewater
  JOURNAL   Microbiol Spectr 12 (3), e0291823 (2024)
   PUBMED   38289113
REFERENCE   2  (bases 1 to 2190)
  AUTHORS   Abdulkadir,N.
  TITLE     Direct Submission
  JOURNAL   Submitted (24-MAR-2023) Environmental Microbiology, Helmholtz
            Center for Environmental Research -UFZ, Permoserstr 15, Leipzig
            04318, Germany
COMMENT     The annotation was added by the NCBI Prokaryotic Genome Annotation
            Pipeline (PGAP). Information about PGAP can be found here:
            https://www.ncbi.nlm.nih.gov/genome/annotation_prok/
            
            ##Genome-Assembly-Data-START##
            Assembly Method        :: SPAdes v. 3.11.1
            Genome Representation  :: Full
            Expected Final Version :: Yes
            Genome Coverage        :: Not Applicable
            Sequencing Technology  :: Illumina MiSeq
            ##Genome-Assembly-Data-END##
            
            ##Genome-Annotation-Data-START##
            Annotation Provider               :: NCBI
            Annotation Date                   :: 05/04/2023 04:57:10
            Annotation Pipeline               :: NCBI Prokaryotic Genome
                                                 Annotation Pipeline (PGAP)
            Annotation Method                 :: Best-placed reference protein
                                                 set; GeneMarkS-2+
            Annotation Software revision      :: 6.5
            Features Annotated                :: Gene; CDS; rRNA; tRNA; ncRNA
            Genes (total)                     :: 2,776
            CDSs (total)                      :: 2,750
            Genes (coding)                    :: 2,714
            CDSs (with protein)               :: 2,714
            Genes (RNA)                       :: 26
            tRNAs                             :: 24
            ncRNAs                            :: 2
            Pseudo Genes (total)              :: 36
            CDSs (without protein)            :: 36
            Pseudo Genes (ambiguous residues) :: 0 of 36
            Pseudo Genes (frameshifted)       :: 2 of 36
            Pseudo Genes (incomplete)         :: 32 of 36
            Pseudo Genes (internal stop)      :: 2 of 36
            ##Genome-Annotation-Data-END##
FEATURES             Location/Qualifiers
     source          1..2190
                     /organism="Micropruina sp."
                     /mol_type="genomic DNA"
                     /submitter_seqid="NODE_20229_length_2190_cov_3.969555"
                     /isolate="AMR_MDS_2195"
                     /isolation_source="Activated sludge of municipal
                     wastewater treatment plants"
                     /db_xref="taxon:2737536"
                     /environmental_sample
                     /geo_loc_name="Canada"
                     /lat_lon="49.25 N 123.1 W"
                     /collection_date="2015-06-16"
                     /metagenome_source="wastewater metagenome"
                     /note="metagenomic"
     gene            <1..187
                     /locus_tag="PLF56_13385"
     CDS             <1..187
                     /locus_tag="PLF56_13385"
                     /inference="COORDINATES: ab initio
                     prediction:GeneMarkS-2+"
                     /note="Derived by automated computational analysis using
                     gene prediction method: GeneMarkS-2+."
                     /codon_start=2
                     /transl_table=11
                     /product="hypothetical protein"
                     /protein_id="HOQ54591.1"
                     /translation="VGIVFFLGLVIAIASLVAGRIVNMPHATKMGIVGIVMVIVGAIA
                     YVVAPGIVNGILGNGCI"
     gene            286..1260
                     /locus_tag="PLF56_13390"
     CDS             286..1260
                     /locus_tag="PLF56_13390"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_000082736.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_function: GO:0004803 - transposase activity [Evidence
                     IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="IS5 family transposase"
                     /protein_id="HOQ54592.1"
                     /translation="MKGSQPSFTDAEYSRRRRVTRREEFLTMMDQVIPWGEWVGLIEP
                     HYYNKTRGRRPVPIETMLRMYLLQVWFNLSDEAVEDQINDSYAMRQFMGLDFAVTQVP
                     DATTLLHFRHLVEKHQLGEAMFAALNRLFEANGWIMRGGSVVDATIINAPSSTKNAAG
                     VRDPEMHQTRKGNQWYFGMKAHVGVDAGTGYVHTVTATAANVHDLEQIAHLVRADDQI
                     VYADAGYQGVERRPDIVADGHLSTVEWRVAARKGVLKTMPGPDRQMESRKASVRAKVE
                     HPFLILKRDFGFAKTRYRGMAKNLNRLHMGFASANLLMRARAVAIMAG"
     gene            complement(1385..1762)
                     /locus_tag="PLF56_13395"
     CDS             complement(1385..1762)
                     /locus_tag="PLF56_13395"
                     /inference="COORDINATES: protein motif:HMM:NF012655.2"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_function: GO:0003677 - DNA binding [Evidence IEA];
                     GO_function: GO:0003700 - DNA-binding transcription factor
                     activity [Evidence IEA];
                     GO_process: GO:0006355 - regulation of DNA-templated
                     transcription [Evidence IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="TetR/AcrR family transcriptional regulator"
                     /protein_id="HOQ54593.1"
                     /translation="MTQVTSEPVSGLRERKKSATRTAIHETALRLVAERGPQSVTVEE
                     ICAEVGVSPRTFFNYYPSKVAAAFDLLVTDIPADQVEWFLNADGSLVADTCDLIGRNV
                     TMPTDFPRIKALMHGQPELLRIR"
     gene            complement(1759..>2190)
                     /locus_tag="PLF56_13400"
     CDS             complement(1759..>2190)
                     /locus_tag="PLF56_13400"
                     /inference="COORDINATES: ab initio
                     prediction:GeneMarkS-2+"
                     /note="Derived by automated computational analysis using
                     gene prediction method: GeneMarkS-2+."
                     /codon_start=1
                     /transl_table=11
                     /product="MFS transporter"
                     /protein_id="HOQ54594.1"
                     /translation="LIDKAVKKALPDAYAKALQQVAFERNLSVKDGVLAIDWSDPVQR
                     WAMVDELTPTLVQTIKNGDAAGSDVNTDSSTSDTSFLTGADPRLTQPFMVGFSDSVIT
                     IYWVGLGVMVLAFVVTLFFKVPPLRTRSALQEKSDALEGLA"
BASE COUNT          385 a          647 c          757 g          401 t
ORIGIN      
        1 cgtggggatc gtgttcttcc tcggcttggt gatcgcgatc gcttcgctgg tggcgggccg
       61 gatcgtgaac atgccgcacg ccacgaagat gggcatcgtc ggcatcgtca tggtgatcgt
      121 cggcgcgatc gcgtacgtgg tcgcccctgg gatcgtcaac ggcatcctcg gcaatggctg
      181 catctaggga aacgctgatc aatgttcgag ctgttgcgct cgggtggtgg cgttgttgac
      241 tgtgttggtc ggttgattgt ggctgtggct aggtagaatg gtgtcatgaa ggggtcgcag
      301 ccgagtttca ctgatgccga gtacagccgt cgccgcaggg tgactcgtcg ggaggagttc
      361 ctgacgatga tggaccaggt gatcccgtgg ggtgagtggg tggggttgat cgagccgcac
      421 tattacaaca agacgcgggg ccgcaggccg gtgccgatcg agacgatgct gcggatgtat
      481 ctgttgcagg tgtggttcaa cctgtccgat gaggcggtgg aggaccagat caacgactcg
      541 tatgcgatgc gccagttcat gggactggac ttcgccgtga cgcaggtccc ggacgccacc
      601 accctgctcc acttccgtca cctggtggag aagcatcagt tgggggaggc gatgttcgcg
      661 gcgttgaacc ggctgttcga ggccaacggg tggatcatgc gcgggggcag tgtggtggac
      721 gccacgatca tcaacgcccc gtcctcgacg aagaacgcgg cgggggtcag ggatccggag
      781 atgcatcaga cccgcaaggg gaaccagtgg tacttcggga tgaaggccca cgtcggggtg
      841 gatgccggga ccggctacgt ccatacggtc accgccacgg ccgcgaacgt gcacgacctc
      901 gaacagatcg cccacctggt gcgtgccgat gatcagatcg tgtatgccga tgccggctac
      961 cagggtgtgg agcggcgacc cgacattgtg gctgacgggc acctgtcgac ggtcgagtgg
     1021 cgggtcgcgg cccgtaaagg ggtgttgaag acgatgcccg ggccggaccg gcagatggag
     1081 tcccgcaagg cgtcggtgcg ggccaaggtg gagcatccgt tcctgatcct gaaacgcgat
     1141 ttcgggttcg ccaagacccg ttaccgcggg atggcgaaga acctgaaccg gctccacatg
     1201 gggttcgcct cagcgaacct gctgatgaga gcacgggccg tcgcgatcat ggccgggtag
     1261 tcgggcggca gcctgcccga aaccggccgc agaccccgga atcgggaacc tgcaggaggc
     1321 cgggagcccg ctcacccaca cgagaacgcc cactcagcgt ccgcgcgcca acaagcccca
     1381 atgatcagcg aatccttagg agttccggct ggccgtgcat cagggctttg atccgaggga
     1441 agtcggtggg catggtgacg ttgcgtccga tcaggtcaca ggtgtcggcg accagacttc
     1501 cgtccgcatt caggaaccat tccacctggt cggccgggat gtcggtgacc agcaggtcga
     1561 acgcggccgc taccttgctc gggtagtagt tgaagaaagt tcgcggcgag accccgacct
     1621 cggcgcagat ctcctcgaca gtgacgcttt gcgggccgcg ctcggcaacc aaacggaggg
     1681 ccgtctcgtg aatcgccgtc cgcgtcgccg acttcttgcg ctcgcgcaga ccactgaccg
     1741 gttcgctggt cacctgcgtc atgcgagtcc ctccagagcg tccgacttct cctgcagcgc
     1801 ggagcgggtg cgcagcggcg gcaccttgaa gaacagggtg accacgaagg cgagcaccat
     1861 gacccccagc cccacccagt agatcgtaat caccgaatcg ctgaagccca ccatgaaggg
     1921 ttgggtgagc cgcgggtcgg ccccggtgag gaacgaggtg tcgctggtgc tgctgtcggt
     1981 gttcacgtcg ctgcctgcgg cgtcaccgtt cttgatcgtc tgcaccagtg tcggcgtgag
     2041 ctcgtcgacc atcgcccagc gctgcaccgg atcggaccag tcgatggcga gcacgccatc
     2101 cttgacgctc aggttccgct cgaaagcgac ctgctgcaag gccttcgcgt aggcgtcggg
     2161 cagtgccttc ttgacggctt tgtcgatcag
//
LOCUS       DAOSUG010000507         2185 bp    DNA     linear   ENV 10-JUN-2024
DEFINITION  MAG TPA_asm: Micropruina sp. isolate AMR_MDS_2195
            NODE_20287_length_2185_cov_3.625822, whole genome shotgun sequence.
ACCESSION   DAOSUG010000507 DAOSUG010000000
VERSION     DAOSUG010000507.1
DBLINK      BioProject: PRJNA925477
            BioSample: SAMN32864262
            Sequence Read Archive: SRR4236673
KEYWORDS    WGS; ENV; Metagenome Assembled Genome; MAG; Third Party Data; TPA;
            TPA:assembly.
SOURCE      Micropruina sp. (wastewater metagenome)
  ORGANISM  Micropruina sp.
            Bacteria; Bacillati; Actinomycetota; Actinomycetes;
            Propionibacteriales; Nocardioidaceae; Micropruina.
REFERENCE   1  (bases 1 to 2185)
  AUTHORS   Abdulkadir,N., Saraiva,J.P., Zhang,J., Stolte,S., Gillor,O.,
            Harms,H. and Rocha,U.
  TITLE     Genome-centric analyses of 165 metagenomes show that mobile genetic
            elements are crucial for the transmission of antimicrobial
            resistance genes to pathogens in activated sludge and wastewater
  JOURNAL   Microbiol Spectr 12 (3), e0291823 (2024)
   PUBMED   38289113
REFERENCE   2  (bases 1 to 2185)
  AUTHORS   Abdulkadir,N.
  TITLE     Direct Submission
  JOURNAL   Submitted (24-MAR-2023) Environmental Microbiology, Helmholtz
            Center for Environmental Research -UFZ, Permoserstr 15, Leipzig
            04318, Germany
COMMENT     The annotation was added by the NCBI Prokaryotic Genome Annotation
            Pipeline (PGAP). Information about PGAP can be found here:
            https://www.ncbi.nlm.nih.gov/genome/annotation_prok/
            
            ##Genome-Assembly-Data-START##
            Assembly Method        :: SPAdes v. 3.11.1
            Genome Representation  :: Full
            Expected Final Version :: Yes
            Genome Coverage        :: Not Applicable
            Sequencing Technology  :: Illumina MiSeq
            ##Genome-Assembly-Data-END##
            
            ##Genome-Annotation-Data-START##
            Annotation Provider               :: NCBI
            Annotation Date                   :: 05/04/2023 04:57:10
            Annotation Pipeline               :: NCBI Prokaryotic Genome
                                                 Annotation Pipeline (PGAP)
            Annotation Method                 :: Best-placed reference protein
                                                 set; GeneMarkS-2+
            Annotation Software revision      :: 6.5
            Features Annotated                :: Gene; CDS; rRNA; tRNA; ncRNA
            Genes (total)                     :: 2,776
            CDSs (total)                      :: 2,750
            Genes (coding)                    :: 2,714
            CDSs (with protein)               :: 2,714
            Genes (RNA)                       :: 26
            tRNAs                             :: 24
            ncRNAs                            :: 2
            Pseudo Genes (total)              :: 36
            CDSs (without protein)            :: 36
            Pseudo Genes (ambiguous residues) :: 0 of 36
            Pseudo Genes (frameshifted)       :: 2 of 36
            Pseudo Genes (incomplete)         :: 32 of 36
            Pseudo Genes (internal stop)      :: 2 of 36
            ##Genome-Annotation-Data-END##
FEATURES             Location/Qualifiers
     source          1..2185
                     /organism="Micropruina sp."
                     /mol_type="genomic DNA"
                     /submitter_seqid="NODE_20287_length_2185_cov_3.625822"
                     /isolate="AMR_MDS_2195"
                     /isolation_source="Activated sludge of municipal
                     wastewater treatment plants"
                     /db_xref="taxon:2737536"
                     /environmental_sample
                     /geo_loc_name="Canada"
                     /lat_lon="49.25 N 123.1 W"
                     /collection_date="2015-06-16"
                     /metagenome_source="wastewater metagenome"
                     /note="metagenomic"
     gene            <1..325
                     /locus_tag="PLF56_13405"
     CDS             <1..325
                     /locus_tag="PLF56_13405"
                     /inference="COORDINATES: protein motif:HMM:NF040015.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=2
                     /transl_table=11
                     /product="DUF6177 family protein"
                     /protein_id="HOQ54595.1"
                     /translation="VESRHQPTIGFVSLAEVATGVAQRVGVRRPEAPLAVLIGPRAVH
                     DLQPQVADLKQRHDVLELGRRRLPSLLVRFSRTDQGLWSQLMAFAGDLGLERVREVAG
                     LVAGS"
     gene            327..662
                     /locus_tag="PLF56_13410"
     CDS             327..662
                     /locus_tag="PLF56_13410"
                     /inference="COORDINATES: ab initio
                     prediction:GeneMarkS-2+"
                     /note="Derived by automated computational analysis using
                     gene prediction method: GeneMarkS-2+."
                     /codon_start=1
                     /transl_table=11
                     /product="hypothetical protein"
                     /protein_id="HOQ54596.1"
                     /translation="MPTELTLLSEVEPSLEAIVTIAATVHPDGAYVQYRGGDIKQFVD
                     DSGTPLLCLFGTRPVIEAREAAAALADPPAAFGLWTDLTLPFGDDGRGRRLAEAIAAG
                     LRGRLAERN"
     gene            675..1040
                     /locus_tag="PLF56_13415"
     CDS             675..1040
                     /locus_tag="PLF56_13415"
                     /inference="COORDINATES: protein motif:HMM:NF040449.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="DUF6507 family protein"
                     /protein_id="HOQ54597.1"
                     /translation="MSNWNIQPTEVQTVLTTVQTKSDTLGKALTEEKFTAIGNGIIWG
                     SVLTEEVSKAVNQVMTEQGENLRVIQNHIGAGSAGVANATIAYNNGQLDMAATFQSEA
                     VKAADTGNFQYFADHGYQG"
     gene            1050..>2185
                     /locus_tag="PLF56_13420"
     CDS             1050..>2185
                     /locus_tag="PLF56_13420"
                     /inference="COORDINATES: ab initio
                     prediction:GeneMarkS-2+"
                     /note="Derived by automated computational analysis using
                     gene prediction method: GeneMarkS-2+."
                     /codon_start=1
                     /transl_table=11
                     /product="hypothetical protein"
                     /protein_id="HOQ54598.1"
                     /translation="MTCTDGGGGSSNGATGGDGLVDPAGLIAKSADLDSTAITGAADG
                     IRSMGSTVDTQTDAIKTEWSTKLPGCYEAPEQETVYALMTEPATASEQLKTTFEAMAG
                     FLDTYAAALEAIKPDLSDWETRAQAFRDRVIDGVWVNKNEAKDSSLLDDGVALWNSMW
                     GNEQEKVKVSWKEDTGTVEENEAYIDEIAAIYARVSKAASICATSINGLTSLPADKKV
                     IPPVPKEAFTNPEAPMPWGYAAQEERNCRESTGEGFYQFGKGTVEGLGSLISYNPETG
                     DWGDWGHAGQAWMGTGNLLLSLAVTASPVAQLLNGALKMTGNGDSAVSQWIDERNQMT
                     ATVGAGLVGIDLNADDPFHKWKEDGVATFTESFLNVGTMFIPGAG"
BASE COUNT          411 a          698 c          770 g          306 t
ORIGIN      
        1 ggtggagagc cgtcaccagc ccacgatcgg gttcgtgtcg ctggccgagg tggccacggg
       61 cgtggcccag cgggtcggcg ttcggcgtcc ggaggcaccc ctcgcggtgt tgatcggacc
      121 gcgggccgtc cacgacctgc agccgcaggt cgccgacctg aagcagcggc acgacgtgct
      181 cgaactgggc cgtcgccggc tgcccagcct gctggtgcgg ttcagccgca ccgatcaggg
      241 cctgtggtct cagttgatgg ccttcgccgg cgacttgggg ctcgagcgcg tccgtgaggt
      301 cgccggactg gtagcaggga gttgagatgc ccaccgaact gacgctgtta agtgaggtcg
      361 aaccgagcct cgaggcgatc gtgacgatcg ccgcgaccgt gcaccccgac ggagcctacg
      421 tgcagtaccg cggcggtgac atcaagcagt tcgtggacga ttcgggcacc ccgctgctgt
      481 gcctgttcgg cacccgtccg gtgatcgagg cgcgcgaggc ggcggcggca ttggccgacc
      541 cgccggcagc cttcgggctg tggacggacc tcacgctgcc gttcggcgac gacggcaggg
      601 ggcggcggct ggccgaggcg atcgcagccg gcctgcgcgg ccggctcgcg gaacggaact
      661 gaggggagcg acacgtgtcg aattggaaca tccagccgac cgaggtgcag accgtcctga
      721 ccaccgtgca gaccaagtcg gacacgctgg gcaaggcgtt gaccgaggag aagttcacgg
      781 cgatcggcaa cgggatcatc tggggcagcg tgctgaccga agaggtgagc aaggccgtca
      841 atcaggtgat gaccgagcag ggcgagaacc tgcgggtgat ccagaaccac atcggtgccg
      901 ggtcggccgg ggtggccaac gccaccatcg cctacaacaa cggtcagctc gacatggctg
      961 ccacgtttca gagcgaggcc gtcaaggccg ccgacaccgg caactttcag tacttcgccg
     1021 accacggata ccaggggtag agccacatga tgacgtgcac ggacggtggt ggcggcagca
     1081 gcaacggtgc caccggcggc gacggcctgg tcgacccggc cggcctgatc gccaagtcgg
     1141 cggatctgga cagcaccgcg atcacgggcg cggccgacgg catcaggtcg atgggcagca
     1201 ccgtcgacac gcagaccgat gccatcaaga ccgagtggag caccaagctc cccggttgct
     1261 acgaagcgcc cgagcaagag acggtctacg ccctgatgac cgaaccggcc accgcgtcgg
     1321 agcagttgaa gaccaccttc gaggcgatgg ccggctttct cgacacctat gccgcggcgc
     1381 tcgaggcgat caagcccgac ctgtccgact gggagacgcg ggcccaggcc ttccgtgacc
     1441 gcgtgatcga cggcgtctgg gtgaacaaga acgaggccaa ggactccagc ctgctcgacg
     1501 acggcgtcgc gctgtggaac agcatgtggg gcaacgagca agagaaggtc aaggtcagct
     1561 ggaaggagga caccggcacc gtcgaagaga atgaggccta catcgacgag atcgcggcca
     1621 tctacgcgcg ggtgtcgaag gccgcatcca tctgtgccac ctcgatcaac gggctgacca
     1681 gcctgccggc ggacaagaag gtcatcccgc cggtccccaa ggaggcgttc accaatcccg
     1741 aggcgcccat gccgtggggc tacgcggcgc aggaggagcg caactgtcgc gagtccaccg
     1801 gcgagggctt ctatcagttc ggcaagggca cggtcgaagg gctgggttca ctgatcagct
     1861 acaaccccga aaccggtgat tggggtgact gggggcatgc cggacaggcc tggatgggca
     1921 ccggcaacct gctgctgtca ctggcggtga ccgcgagccc ggtcgcccag ttgctgaacg
     1981 gtgcgctcaa gatgaccggc aacggcgaca gcgcggtgtc ccagtggatc gacgaacgca
     2041 accagatgac cgccaccgtc ggcgcgggcc tggtcggcat cgacctgaac gccgatgatc
     2101 cgttccacaa gtggaaggaa gacggcgtcg ccaccttcac cgagtcgttc ctcaacgtgg
     2161 gcaccatgtt cattccgggc gccgg
//
LOCUS       DAOSUG010000508         2185 bp    DNA     linear   ENV 10-JUN-2024
DEFINITION  MAG TPA_asm: Micropruina sp. isolate AMR_MDS_2195
            NODE_20288_length_2185_cov_3.124883, whole genome shotgun sequence.
ACCESSION   DAOSUG010000508 DAOSUG010000000
VERSION     DAOSUG010000508.1
DBLINK      BioProject: PRJNA925477
            BioSample: SAMN32864262
            Sequence Read Archive: SRR4236673
KEYWORDS    WGS; ENV; Metagenome Assembled Genome; MAG; Third Party Data; TPA;
            TPA:assembly.
SOURCE      Micropruina sp. (wastewater metagenome)
  ORGANISM  Micropruina sp.
            Bacteria; Bacillati; Actinomycetota; Actinomycetes;
            Propionibacteriales; Nocardioidaceae; Micropruina.
REFERENCE   1  (bases 1 to 2185)
  AUTHORS   Abdulkadir,N., Saraiva,J.P., Zhang,J., Stolte,S., Gillor,O.,
            Harms,H. and Rocha,U.
  TITLE     Genome-centric analyses of 165 metagenomes show that mobile genetic
            elements are crucial for the transmission of antimicrobial
            resistance genes to pathogens in activated sludge and wastewater
  JOURNAL   Microbiol Spectr 12 (3), e0291823 (2024)
   PUBMED   38289113
REFERENCE   2  (bases 1 to 2185)
  AUTHORS   Abdulkadir,N.
  TITLE     Direct Submission
  JOURNAL   Submitted (24-MAR-2023) Environmental Microbiology, Helmholtz
            Center for Environmental Research -UFZ, Permoserstr 15, Leipzig
            04318, Germany
COMMENT     The annotation was added by the NCBI Prokaryotic Genome Annotation
            Pipeline (PGAP). Information about PGAP can be found here:
            https://www.ncbi.nlm.nih.gov/genome/annotation_prok/
            
            ##Genome-Assembly-Data-START##
            Assembly Method        :: SPAdes v. 3.11.1
            Genome Representation  :: Full
            Expected Final Version :: Yes
            Genome Coverage        :: Not Applicable
            Sequencing Technology  :: Illumina MiSeq
            ##Genome-Assembly-Data-END##
            
            ##Genome-Annotation-Data-START##
            Annotation Provider               :: NCBI
            Annotation Date                   :: 05/04/2023 04:57:10
            Annotation Pipeline               :: NCBI Prokaryotic Genome
                                                 Annotation Pipeline (PGAP)
            Annotation Method                 :: Best-placed reference protein
                                                 set; GeneMarkS-2+
            Annotation Software revision      :: 6.5
            Features Annotated                :: Gene; CDS; rRNA; tRNA; ncRNA
            Genes (total)                     :: 2,776
            CDSs (total)                      :: 2,750
            Genes (coding)                    :: 2,714
            CDSs (with protein)               :: 2,714
            Genes (RNA)                       :: 26
            tRNAs                             :: 24
            ncRNAs                            :: 2
            Pseudo Genes (total)              :: 36
            CDSs (without protein)            :: 36
            Pseudo Genes (ambiguous residues) :: 0 of 36
            Pseudo Genes (frameshifted)       :: 2 of 36
            Pseudo Genes (incomplete)         :: 32 of 36
            Pseudo Genes (internal stop)      :: 2 of 36
            ##Genome-Annotation-Data-END##
FEATURES             Location/Qualifiers
     source          1..2185
                     /organism="Micropruina sp."
                     /mol_type="genomic DNA"
                     /submitter_seqid="NODE_20288_length_2185_cov_3.124883"
                     /isolate="AMR_MDS_2195"
                     /isolation_source="Activated sludge of municipal
                     wastewater treatment plants"
                     /db_xref="taxon:2737536"
                     /environmental_sample
                     /geo_loc_name="Canada"
                     /lat_lon="49.25 N 123.1 W"
                     /collection_date="2015-06-16"
                     /metagenome_source="wastewater metagenome"
                     /note="metagenomic"
     gene            complement(<1..2159)
                     /locus_tag="PLF56_13425"
     CDS             complement(<1..2159)
                     /locus_tag="PLF56_13425"
                     /inference="COORDINATES: protein motif:HMM:NF012602.2"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_function: GO:0016491 - oxidoreductase activity
                     [Evidence IEA];
                     GO_function: GO:0043546 - molybdopterin cofactor binding
                     [Evidence IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="molybdopterin dinucleotide binding
                     domain-containing protein"
                     /protein_id="HOQ54599.1"
                     /translation="MGGREMGYMGPGLPGQRSVLDADDRTFTEQAWGLAPGTLRTDVG
                     TGTIDMFRRMADGEIKACWIICTNPVASVANRSQVIAGLERCELVIAQDAFLDTETNA
                     YADVLLPAALWAESTYVTVNSERNLTLLEQAVDPVGEALPDWRIITEMARAMGYGEQF
                     GHESAEQVFDELKGFSNPRTGYDLRGVTYSRLRATPVQWPAPPLAPGAQADVVDDGRN
                     PVRYLNDGISQLPVERPDGVRPRLAFATPSGRANFFARPHMDAKELPDDDYPFVLNTG
                     RVQHHWHTLTKTGKVAKLVKLSPGPFIEVHPDDAARLGIAEDDLVEIASRRGRAVLPA
                     VVSDRVQPGGLFAPFHWNDTFGEYLAVNAVTSDAVDPTSFQPEFKVAAVRLTRVGPAP
                     DPVTTQPSTAAPDVLVPGAPEPMPLSGAATAAATASSARWRESALAGIGLTPAPPPAF
                     TTAQAQFLQGLLWARQQDPSPEAPVLPAAAPFDADTRAWVEGWLAGVVGPAQLPAAPE
                     APEAPRRRVVMLWASQTGNAEDHAARYAGQLRGAGFEVDLVEMDSFDAVRLQSSSVVL
                     LVTSTFGDGESPDDGERFWSDLTGAGVAPLPDVRFAVLAFGDSSYATFCGHGRRLDER
                     LAELGAVRITERGECEPGDSEPADAWFDRVVGWLDQADPPSGPPPAQTTYVGEPTAAV
                     PAAPATNGAAVYSRTSPLVTRLTRNHRLGAPSSTKDVR"
BASE COUNT          292 a          736 c          854 g          303 t
ORIGIN      
        1 cgcacgtcct tggtcgagct gggcgcaccg agccggtggt tgcgggtgag ccgggtgacc
       61 agcggcgagg tgcgtgagta cacggcggcg ccgttggtgg ccggcgctgc gggtacggcg
      121 gctgtgggtt caccgacgta ggtggtctgt gcgggcggcg gaccggacgg cgggtcggcc
      181 tggtcgagcc agccgaccac ccggtcgaac caggcgtcgg cgggttcgct gtcgcccggt
      241 tcgcactcgc cccgctcggt gatccgcacc gcgcccaact cggccagccg ctcgtccagc
      301 cggcgcccgt gaccacagaa agtggcatag ctggagtcgc cgaaggccag cacggcgaag
      361 cgcacgtccg gcaggggggc gacgccggcg ccggtgaggt cggaccagaa ccgctccccg
      421 tcatcgggcg actcgccgtc gccgaacgtg ctggtcacca gcagcaccac cgaactcgac
      481 tgcaggcgca cggcgtcgaa ggagtccatc tcgaccaggt cgacctcgaa gccggcgccg
      541 cgcaactggc cggcgtagcg ggcggcatgg tcctcggcgt tgccggtctg ggacgcccac
      601 agcatcacca cccgccgccg gggggcctcc ggcgcctcgg gtgcggccgg gagctgcgcc
      661 ggaccgacga caccggccag ccagccctcg acccaggccc gggtgtcggc atcgaagggg
      721 gccgccgcgg gcagcaccgg ggcctcgggc gaggggtcct gctgccgggc ccacagcagg
      781 ccctgcagaa actgcgcctg ggcggtggtg aacgccggcg gcggggcggg ggtgagtccg
      841 atgccggcca gcgcgctctc gcgccagcgg gcggacgacg cggtggcagc ggcggtggca
      901 gcgccggaga gcggcatcgg ttccggcgcc ccgggcacga gcacgtccgg tgcagcggtg
      961 gagggttggg tcgtgaccgg atcgggcgcg ggtccgaccc gggtcagccg cacggctgcc
     1021 accttgaact cgggctggaa cgaggtggga tcgacggcgt cggaggtgac cgcgttgacc
     1081 gccaggtact cgccgaaggt gtcgttccag tggaacggtg cgaacaggcc gccgggctgc
     1141 acccggtcgg agacgacggc gggcagcacg gcgcggccgc ggcgggaggc gatctcgacc
     1201 aggtcatcct cggcgatgcc gagccgggcc gcgtcgtcgg gatgtacctc gatgaagggg
     1261 cccgggctga gcttgaccag tttcgccacc ttgccggtct tggtcagcgt gtgccagtgg
     1321 tgctgcacgc ggccggtgtt cagcacgaac gggtagtcgt cgtcgggcaa ctccttggcg
     1381 tccatgtgcg gtcgggcgaa gaagttcgcc cgtccgctcg gggtcgcaaa cgccagccgg
     1441 ggacgcaccc cgtccgggcg ctcgacgggc aactggctga tgccgtcgtt gaggtagcgg
     1501 acggggttgc ggccgtcgtc gacgacatcg gcttgcgcgc ccggggccag cggcggggcc
     1561 ggccactgca cgggcgtggc ccgcaaccgg gaataggtca cgccgcgcag gtcgtagccg
     1621 gtgcgcgggt tgctgaagcc cttgagctcg tcgaagacct gctcggcgct ttcgtggccg
     1681 aactgctcgc cgtagcccat cgcgcgggcc atctcggtga tgatccgcca gtccggcagc
     1741 gcctcgccga ccgggtccac cgcctgctcg agcagggtca ggttgcgttc ggagttgacc
     1801 gtgacgtagg tcgactcggc ccacagcgct gccggcagca gcacgtcggc gtaggcgttg
     1861 gtctcggtgt cgagaaaggc gtcctgggcg atcaccaact cgcagcgttc cagccccgcg
     1921 atcacctggg agcggttggc gaccgaggcg accgggttgg tgcagatgat ccagcaggcc
     1981 ttgatctcgc cgtcggccat gcggcggaac atgtcgatgg tgccggtgcc gacgtcggtg
     2041 cgcagggtgc ccggggccag tccccaggcc tgctcggtga aggtgcggtc gtcggcgtcg
     2101 agcacgctgc gctggcccgg caggcccgga cccatgtagc ccatctcgcg gccgcccatc
     2161 gcgttgggct ggccggtcag cgaga
//
LOCUS       DAOSUG010000509         2148 bp    DNA     linear   ENV 10-JUN-2024
DEFINITION  MAG TPA_asm: Micropruina sp. isolate AMR_MDS_2195
            NODE_20750_length_2148_cov_3.126613, whole genome shotgun sequence.
ACCESSION   DAOSUG010000509 DAOSUG010000000
VERSION     DAOSUG010000509.1
DBLINK      BioProject: PRJNA925477
            BioSample: SAMN32864262
            Sequence Read Archive: SRR4236673
KEYWORDS    WGS; ENV; Metagenome Assembled Genome; MAG; Third Party Data; TPA;
            TPA:assembly.
SOURCE      Micropruina sp. (wastewater metagenome)
  ORGANISM  Micropruina sp.
            Bacteria; Bacillati; Actinomycetota; Actinomycetes;
            Propionibacteriales; Nocardioidaceae; Micropruina.
REFERENCE   1  (bases 1 to 2148)
  AUTHORS   Abdulkadir,N., Saraiva,J.P., Zhang,J., Stolte,S., Gillor,O.,
            Harms,H. and Rocha,U.
  TITLE     Genome-centric analyses of 165 metagenomes show that mobile genetic
            elements are crucial for the transmission of antimicrobial
            resistance genes to pathogens in activated sludge and wastewater
  JOURNAL   Microbiol Spectr 12 (3), e0291823 (2024)
   PUBMED   38289113
REFERENCE   2  (bases 1 to 2148)
  AUTHORS   Abdulkadir,N.
  TITLE     Direct Submission
  JOURNAL   Submitted (24-MAR-2023) Environmental Microbiology, Helmholtz
            Center for Environmental Research -UFZ, Permoserstr 15, Leipzig
            04318, Germany
COMMENT     The annotation was added by the NCBI Prokaryotic Genome Annotation
            Pipeline (PGAP). Information about PGAP can be found here:
            https://www.ncbi.nlm.nih.gov/genome/annotation_prok/
            
            ##Genome-Assembly-Data-START##
            Assembly Method        :: SPAdes v. 3.11.1
            Genome Representation  :: Full
            Expected Final Version :: Yes
            Genome Coverage        :: Not Applicable
            Sequencing Technology  :: Illumina MiSeq
            ##Genome-Assembly-Data-END##
            
            ##Genome-Annotation-Data-START##
            Annotation Provider               :: NCBI
            Annotation Date                   :: 05/04/2023 04:57:10
            Annotation Pipeline               :: NCBI Prokaryotic Genome
                                                 Annotation Pipeline (PGAP)
            Annotation Method                 :: Best-placed reference protein
                                                 set; GeneMarkS-2+
            Annotation Software revision      :: 6.5
            Features Annotated                :: Gene; CDS; rRNA; tRNA; ncRNA
            Genes (total)                     :: 2,776
            CDSs (total)                      :: 2,750
            Genes (coding)                    :: 2,714
            CDSs (with protein)               :: 2,714
            Genes (RNA)                       :: 26
            tRNAs                             :: 24
            ncRNAs                            :: 2
            Pseudo Genes (total)              :: 36
            CDSs (without protein)            :: 36
            Pseudo Genes (ambiguous residues) :: 0 of 36
            Pseudo Genes (frameshifted)       :: 2 of 36
            Pseudo Genes (incomplete)         :: 32 of 36
            Pseudo Genes (internal stop)      :: 2 of 36
            ##Genome-Annotation-Data-END##
FEATURES             Location/Qualifiers
     source          1..2148
                     /organism="Micropruina sp."
                     /mol_type="genomic DNA"
                     /submitter_seqid="NODE_20750_length_2148_cov_3.126613"
                     /isolate="AMR_MDS_2195"
                     /isolation_source="Activated sludge of municipal
                     wastewater treatment plants"
                     /db_xref="taxon:2737536"
                     /environmental_sample
                     /geo_loc_name="Canada"
                     /lat_lon="49.25 N 123.1 W"
                     /collection_date="2015-06-16"
                     /metagenome_source="wastewater metagenome"
                     /note="metagenomic"
     gene            <1..1903
                     /gene="manA"
                     /locus_tag="PLF56_13430"
     CDS             <1..1903
                     /gene="manA"
                     /locus_tag="PLF56_13430"
                     /EC_number="5.3.1.8"
                     /inference="COORDINATES: protein motif:HMM:TIGR00218.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_function: GO:0004476 - mannose-6-phosphate isomerase
                     activity [Evidence IEA];
                     GO_process: GO:0005975 - carbohydrate metabolic process
                     [Evidence IEA]"
                     /codon_start=2
                     /transl_table=11
                     /product="mannose-6-phosphate isomerase, class I"
                     /protein_id="HOQ54600.1"
                     /translation="TEEVRLAHDSITSYLAALGPEPGVVVAAGTGVVTLGVGASGTQR
                     VDGWGYLLGDCGSGYWLGRAALHHALRAYDGRGPATALTQVLIDAFPQPEGAYIELQT
                     DPDKVRRIAGFARAVCELAATDEVAASICREAGDELALSAYAAARRVALGDDVRVCLI
                     GGVFEAGAVRAATEAALRTRVPGVRIVAPLGEGIDGAEELARIEPGHPLASSIAIARS
                     SGTRHLFRVQPPLRDYAWGSPTHIPHLLGRQPDGRPQAELWLGAHPSAPATVGGAGLD
                     QVLADRPELISTEPAAGVRAGGGPAPRGLPFLMKVLAAGRPLSLQVHPNREQAAEGYE
                     RENAAGIALGDPRRNYADREHKPELILAITPFVALCGFRAPSQAADDLAELLALTGRD
                     GLAAEPSALAADLLGALREPDESAALRRAVRLLLSGRPAVRELAAAVVQAAHQHSGRY
                     ADTVRLAGDYGDDPGVLAMALLNRVDLAPGEALYLDAGNIHAYLGGLGIEAMAPSDNV
                     LRGGLTPKHVDVDGLLEIVNFAALRPQFVPAQTSESDGVRMDSYWPPVSDFSVHRLLA
                     TGGPRELALTEPAMVIVTDGSLEVSAGSERLVLQRGDSAFHAAGAPLSVRAIEAPAIA
                     YLTTVGVQR"
     gene            complement(1935..>2148)
                     /locus_tag="PLF56_13435"
     CDS             complement(1935..>2148)
                     /locus_tag="PLF56_13435"
                     /inference="COORDINATES: ab initio
                     prediction:GeneMarkS-2+"
                     /note="Derived by automated computational analysis using
                     gene prediction method: GeneMarkS-2+."
                     /codon_start=2
                     /transl_table=11
                     /product="hypothetical protein"
                     /protein_id="HOQ54601.1"
                     /translation="AGPGFATGLFGNTRRVGNSLIGPLIAIAGTPWGYPGVFVACAAL
                     ALVGAATIGVVGRRAARRVRPPGTVG"
BASE COUNT          271 a          754 c          813 g          310 t
ORIGIN      
        1 caccgaagag gtgaggctgg cgcacgactc gatcacctca tacctggccg cgctgggccc
       61 ggagcccggt gtcgtggtgg cggccggcac cggcgtggtg acgctcggcg tgggcgcgtc
      121 cggcacgcaa cgggtggacg gttggggtta cctgctgggt gactgcggca gcggctactg
      181 gctgggccgc gcggccctgc accacgcgct tcgggcctac gacggccggg gtccggcgac
      241 ggcactgacg caggtgctga tcgacgcgtt tccgcagccc gaaggtgcct acatcgagtt
      301 gcagaccgac cctgacaagg tgcgtcgcat cgccggcttc gcccgggccg tctgcgaact
      361 ggccgcgacg gacgaggtcg ccgcgtcgat ctgtcgtgag gcgggcgatg agttggcgct
      421 gtcggcctac gccgcggccc gccgggtcgc cctcggcgac gacgtgcggg tctgcctgat
      481 cggcggcgtg ttcgaggccg gcgccgtgcg agcggccacc gaggctgcgc tgcgcacgcg
      541 ggtgcccggc gtgcgcatcg tcgcaccctt gggtgagggc atcgacggtg ccgaagagct
      601 ggcccggatc gagccgggtc acccgctggc gagcagcatc gcaatcgccc ggtcgtccgg
      661 tacgcggcac ctgttccggg tgcagccccc attgcgtgat tacgcgtggg gttcgcccac
      721 ccacattccc cacctgctcg ggcggcagcc ggacggccga ccgcaggccg aactctggct
      781 gggcgcgcac ccgtcggcgc ccgcgacggt gggcggcgca ggtctggacc aggtgctggc
      841 cgaccggccc gagctgatct cgacagagcc tgccgcggga gtgcgcgctg ggggcgggcc
      901 ggccccgcgc gggctgccgt tcctgatgaa ggtgctggcg gcggggcgtc cgctgtcgtt
      961 gcaggtgcac cccaaccgcg agcaggccgc cgagggctat gagcgtgaga acgcggccgg
     1021 catcgcgctg ggtgatccgc ggcgcaacta cgccgaccgt gagcacaagc ccgaactgat
     1081 tctggcgatc acgccgttcg tggcgctgtg cggattccgc gccccgtcgc aggccgcgga
     1141 cgatctggcc gaactgctcg cgctcaccgg gcgcgacggg ctggccgctg agccgtccgc
     1201 actggccgcg gacctgctgg gggcgctgcg tgagcccgat gagtcggccg cgctgcgccg
     1261 ggccgtgcgg ctgttgctgt cgggacgacc cgcggtgcgc gagttggcgg ccgcggtggt
     1321 gcaggccgcg caccagcact ccggccgcta tgccgacacg gtgcggttgg ccggtgacta
     1381 cggcgacgat ccgggggtgt tggcgatggc cctgctcaac cgggtggatc tggctcccgg
     1441 tgaggccctg tacctcgacg ccggcaacat tcacgcctac ctgggcggcc tgggcatcga
     1501 ggcgatggca ccgtccgaca atgtgttgcg cggcgggctc acgcccaagc acgtcgacgt
     1561 cgacggactg ctcgagatcg tcaacttcgc ggcgctgcgg ccccagttcg tgccggcgca
     1621 gacctcggag agcgacgggg tgcggatgga ttcgtactgg cctcccgtgt cggacttctc
     1681 ggttcaccgg ttgctggcca ccggcggccc gcgcgaactc gccctgaccg agccggcgat
     1741 ggtgatcgtc accgacggca gcctcgaggt gagtgccggc agcgagcgac tcgtgctgca
     1801 gcgcggcgac agtgcctttc acgccgccgg cgcaccattg agcgtccggg cgatcgaggc
     1861 ccccgcgatc gcctacctga ccacagtggg cgtgcagcgc tgatcagtcg ccgctcgagc
     1921 gtgcccgaga ctcctcaccc cacggttccg gggggacgga cgcgccgcgc ggcccgccgt
     1981 ccgaccacac cgatggtggc ggctccgacc agcgccagtg cggcgcatgc gacgaacacg
     2041 cccgggtacc cccacggcgt gcccgcgatg gcgatcaggg gtccgatcag actgttgccc
     2101 acccggcggg tgttgccgaa cagcccggtg gcgaagccgg ggccggcg
//
LOCUS       DAOSUG010000510         2144 bp    DNA     linear   ENV 10-JUN-2024
DEFINITION  MAG TPA_asm: Micropruina sp. isolate AMR_MDS_2195
            NODE_20805_length_2144_cov_3.927238, whole genome shotgun sequence.
ACCESSION   DAOSUG010000510 DAOSUG010000000
VERSION     DAOSUG010000510.1
DBLINK      BioProject: PRJNA925477
            BioSample: SAMN32864262
            Sequence Read Archive: SRR4236673
KEYWORDS    WGS; ENV; Metagenome Assembled Genome; MAG; Third Party Data; TPA;
            TPA:assembly.
SOURCE      Micropruina sp. (wastewater metagenome)
  ORGANISM  Micropruina sp.
            Bacteria; Bacillati; Actinomycetota; Actinomycetes;
            Propionibacteriales; Nocardioidaceae; Micropruina.
REFERENCE   1  (bases 1 to 2144)
  AUTHORS   Abdulkadir,N., Saraiva,J.P., Zhang,J., Stolte,S., Gillor,O.,
            Harms,H. and Rocha,U.
  TITLE     Genome-centric analyses of 165 metagenomes show that mobile genetic
            elements are crucial for the transmission of antimicrobial
            resistance genes to pathogens in activated sludge and wastewater
  JOURNAL   Microbiol Spectr 12 (3), e0291823 (2024)
   PUBMED   38289113
REFERENCE   2  (bases 1 to 2144)
  AUTHORS   Abdulkadir,N.
  TITLE     Direct Submission
  JOURNAL   Submitted (24-MAR-2023) Environmental Microbiology, Helmholtz
            Center for Environmental Research -UFZ, Permoserstr 15, Leipzig
            04318, Germany
COMMENT     The annotation was added by the NCBI Prokaryotic Genome Annotation
            Pipeline (PGAP). Information about PGAP can be found here:
            https://www.ncbi.nlm.nih.gov/genome/annotation_prok/
            
            ##Genome-Assembly-Data-START##
            Assembly Method        :: SPAdes v. 3.11.1
            Genome Representation  :: Full
            Expected Final Version :: Yes
            Genome Coverage        :: Not Applicable
            Sequencing Technology  :: Illumina MiSeq
            ##Genome-Assembly-Data-END##
            
            ##Genome-Annotation-Data-START##
            Annotation Provider               :: NCBI
            Annotation Date                   :: 05/04/2023 04:57:10
            Annotation Pipeline               :: NCBI Prokaryotic Genome
                                                 Annotation Pipeline (PGAP)
            Annotation Method                 :: Best-placed reference protein
                                                 set; GeneMarkS-2+
            Annotation Software revision      :: 6.5
            Features Annotated                :: Gene; CDS; rRNA; tRNA; ncRNA
            Genes (total)                     :: 2,776
            CDSs (total)                      :: 2,750
            Genes (coding)                    :: 2,714
            CDSs (with protein)               :: 2,714
            Genes (RNA)                       :: 26
            tRNAs                             :: 24
            ncRNAs                            :: 2
            Pseudo Genes (total)              :: 36
            CDSs (without protein)            :: 36
            Pseudo Genes (ambiguous residues) :: 0 of 36
            Pseudo Genes (frameshifted)       :: 2 of 36
            Pseudo Genes (incomplete)         :: 32 of 36
            Pseudo Genes (internal stop)      :: 2 of 36
            ##Genome-Annotation-Data-END##
FEATURES             Location/Qualifiers
     source          1..2144
                     /organism="Micropruina sp."
                     /mol_type="genomic DNA"
                     /submitter_seqid="NODE_20805_length_2144_cov_3.927238"
                     /isolate="AMR_MDS_2195"
                     /isolation_source="Activated sludge of municipal
                     wastewater treatment plants"
                     /db_xref="taxon:2737536"
                     /environmental_sample
                     /geo_loc_name="Canada"
                     /lat_lon="49.25 N 123.1 W"
                     /collection_date="2015-06-16"
                     /metagenome_source="wastewater metagenome"
                     /note="metagenomic"
     gene            <1..389
                     /locus_tag="PLF56_13440"
     CDS             <1..389
                     /locus_tag="PLF56_13440"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_014847376.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=3
                     /transl_table=11
                     /product="FAD-dependent oxidoreductase"
                     /protein_id="HOQ54602.1"
                     /translation="LVLVGDSGGMVSPFNGEGIPYAMEAGEAAAEAIGRAHAEGVGTR
                     AAEKALQGYPSRLAAEWGGYYRLGTIFVKLIEHPAVMRLCVRYGLPRPVLMRFTIKLL
                     AHLYDTKDGDWMDKVIHSLTTVVPAA"
     gene            406..765
                     /locus_tag="PLF56_13445"
     CDS             406..765
                     /locus_tag="PLF56_13445"
                     /EC_number="1.6.5.9"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_017948722.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_function: GO:0008137 - NADH dehydrogenase (ubiquinone)
                     activity [Evidence IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="NADH-quinone oxidoreductase subunit A"
                     /protein_id="HOQ54603.1"
                     /translation="MSPYIPILGLMALAAGFLAISVVLSLLVGPRRYNRVKYDTYECG
                     IDPTPAAVGGGRFPVKYYITAMMFLVFDVEVAFLYPWAVSFDALGVFGFAAMMIFLIV
                     LGVAYFYEIRRGGLEWD"
     gene            817..1374
                     /locus_tag="PLF56_13450"
     CDS             817..1374
                     /locus_tag="PLF56_13450"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_020391928.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="NADH-quinone oxidoreductase subunit B family
                     protein"
                     /protein_id="HOQ54604.1"
                     /translation="MGLEDKIPQGVLLTTVEGIFGWMRQASFWPATFGLACCAIEMMT
                     YGAPRFDAGRWGQEVFRASPRQADLMIVSVRVSQKMAPVLRQVYDQMPNPKWVMAMGV
                     CASSGGMFNNYAIVQGGDHIVPVDMYVPGCPPRPDMLIDAMFKLRKQIVMKRPIGAHE
                     LEELEAQEKAALEAMATHEMKGLMR"
     gene            1371..>2144
                     /locus_tag="PLF56_13455"
     CDS             1371..>2144
                     /locus_tag="PLF56_13455"
                     /EC_number="1.6.5.9"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_020578392.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_function: GO:0008137 - NADH dehydrogenase (ubiquinone)
                     activity [Evidence IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="NADH-quinone oxidoreductase subunit C"
                     /protein_id="HOQ54605.1"
                     /translation="MSDNGPEKKLPGEEKSVDEPVEGADSAPGAGLEREDAQPDEALD
                     AVAQVPAGVHVIETRKGMWSDGSGDTSGYGRIVRSIELPNAGLPSEGWYGEATARLLH
                     LVPDALVRVVFDRGELTFFVAREKLTEVMGQLRDDAQLRFEFLPSVSGVNYPDDEGAE
                     LHVVYHLQSMTHNRRLRVETTAPDDDPHVPSVVALYPAADWHERETWDMFGIVFDGHP
                     HLTRILMPDDWVGHPQRKDYPLGGIPVEYKGATVPPPDQR"
BASE COUNT          351 a          701 c          749 g          343 t
ORIGIN      
        1 ggctggtgct ggtcggcgac tccggcggca tggtctcgcc gttcaacggc gagggcatcc
       61 cctacgccat ggaggccggc gaggccgccg ccgaggcgat cggacgggcg cacgccgagg
      121 gtgtcggcac cagggcggcc gagaaggccc tccagggcta cccgtccagg ctcgccgccg
      181 aatggggcgg ttactaccgg ctcggaacga tcttcgtgaa gctgatcgag catccggcgg
      241 tgatgcggct gtgcgtgcgc tacggactgc cgcgtccggt gctgatgagg ttcacgatca
      301 agttgctggc gcacctttat gacaccaaag atggtgattg gatggacaaa gtgatccact
      361 cgctgacgac agtggttccc gcagcatgac cggatggagg catcgatgag tccctatatt
      421 ccgatcctgg ggctgatggc gctggccgcc gggtttctgg cgatctcggt ggtgctcagc
      481 ctgctggtcg gcccgcgccg ctacaaccgg gtcaagtacg acacctacga gtgcggcatc
      541 gaccccaccc cggctgcggt cggcggcggc cggttcccgg tcaagtacta catcaccgcg
      601 atgatgttcc tggtcttcga cgtcgaggtg gccttcctgt acccgtgggc ggtcagcttc
      661 gacgccctgg gggtgttcgg gttcgcggcc atgatgatct tcctgatcgt gctgggcgtc
      721 gcctacttct acgagatccg ccgcggaggg ctggaatggg actgaaatcc ggccggacta
      781 cattgaccgg cgcgcacacg cagcgggagg cgaaccatgg ggcttgagga caagattccg
      841 caaggagtgc tgctgaccac cgtcgagggc atcttcggct ggatgcggca ggcgtcgttc
      901 tggccggcca cgttcggcct ggcctgctgt gcgatcgaga tgatgaccta cggcgcgccc
      961 cgcttcgacg ccggccgttg gggccaggag gtgttccgcg cctcaccccg ccaggccgac
     1021 ctcatgatcg tctcggtccg cgtgtcgcag aagatggccc ccgtgctgcg ccaggtgtac
     1081 gaccagatgc ccaaccccaa gtgggtgatg gcgatgggcg tgtgcgccag ctccggcggc
     1141 atgttcaaca actacgcgat cgtccagggc ggcgaccaca tcgtgccggt cgacatgtac
     1201 gtgcccgggt gcccgccccg tcccgacatg ctgatcgacg ccatgttcaa gctgcgcaag
     1261 cagatcgtga tgaagcgtcc gatcggggcc cacgagctcg aagagctcga ggcgcaggag
     1321 aaggcggcac tcgaggcgat ggccacccac gagatgaagg ggctgatgcg atgagcgaca
     1381 acggtcccga gaagaagctg ccgggcgagg agaagagcgt cgacgaaccc gtcgagggtg
     1441 ccgattcggc acccggcgcc ggcctggagc gcgaggacgc ccagcccgac gaggccctgg
     1501 acgcggtggc gcaggtgccg gccggcgtcc acgtgatcga gacccgcaag ggcatgtggt
     1561 cggacggttc cggcgacacc tccggctacg gacgcatcgt ccgctcgatc gagctgccga
     1621 atgccggcct gcccagcgag ggctggtacg gcgaggcgac ggcgcggctg ctgcacctgg
     1681 tgccggacgc gctggtgcgc gtcgtgttcg accgcggcga gctcaccttc ttcgtcgccc
     1741 gcgagaagct caccgaggtg atggggcagt tgcgcgacga cgcccagttg cggttcgagt
     1801 ttctgcccag cgtgtcgggc gtcaactacc ccgacgacga gggcgccgaa ctgcacgtgg
     1861 tgtaccacct gcagtcgatg acccacaatc gccggctgcg ggtcgagacc accgcacccg
     1921 acgacgaccc gcacgtgccc agcgtggtgg ccctgtaccc ggccgccgac tggcacgagc
     1981 gcgaaacctg ggacatgttc ggcatcgtct tcgacggcca cccgcacctg acccgcattc
     2041 tgatgccgga cgactgggtc gggcacccgc agcgcaagga ctacccgctc ggcggcattc
     2101 cggtggaata caaaggcgcc accgtgccgc cgcccgacca gcgg
//
LOCUS       DAOSUG010000511         2127 bp    DNA     linear   ENV 10-JUN-2024
DEFINITION  MAG TPA_asm: Micropruina sp. isolate AMR_MDS_2195
            NODE_21052_length_2127_cov_3.575290, whole genome shotgun sequence.
ACCESSION   DAOSUG010000511 DAOSUG010000000
VERSION     DAOSUG010000511.1
DBLINK      BioProject: PRJNA925477
            BioSample: SAMN32864262
            Sequence Read Archive: SRR4236673
KEYWORDS    WGS; ENV; Metagenome Assembled Genome; MAG; Third Party Data; TPA;
            TPA:assembly.
SOURCE      Micropruina sp. (wastewater metagenome)
  ORGANISM  Micropruina sp.
            Bacteria; Bacillati; Actinomycetota; Actinomycetes;
            Propionibacteriales; Nocardioidaceae; Micropruina.
REFERENCE   1  (bases 1 to 2127)
  AUTHORS   Abdulkadir,N., Saraiva,J.P., Zhang,J., Stolte,S., Gillor,O.,
            Harms,H. and Rocha,U.
  TITLE     Genome-centric analyses of 165 metagenomes show that mobile genetic
            elements are crucial for the transmission of antimicrobial
            resistance genes to pathogens in activated sludge and wastewater
  JOURNAL   Microbiol Spectr 12 (3), e0291823 (2024)
   PUBMED   38289113
REFERENCE   2  (bases 1 to 2127)
  AUTHORS   Abdulkadir,N.
  TITLE     Direct Submission
  JOURNAL   Submitted (24-MAR-2023) Environmental Microbiology, Helmholtz
            Center for Environmental Research -UFZ, Permoserstr 15, Leipzig
            04318, Germany
COMMENT     The annotation was added by the NCBI Prokaryotic Genome Annotation
            Pipeline (PGAP). Information about PGAP can be found here:
            https://www.ncbi.nlm.nih.gov/genome/annotation_prok/
            
            ##Genome-Assembly-Data-START##
            Assembly Method        :: SPAdes v. 3.11.1
            Genome Representation  :: Full
            Expected Final Version :: Yes
            Genome Coverage        :: Not Applicable
            Sequencing Technology  :: Illumina MiSeq
            ##Genome-Assembly-Data-END##
            
            ##Genome-Annotation-Data-START##
            Annotation Provider               :: NCBI
            Annotation Date                   :: 05/04/2023 04:57:10
            Annotation Pipeline               :: NCBI Prokaryotic Genome
                                                 Annotation Pipeline (PGAP)
            Annotation Method                 :: Best-placed reference protein
                                                 set; GeneMarkS-2+
            Annotation Software revision      :: 6.5
            Features Annotated                :: Gene; CDS; rRNA; tRNA; ncRNA
            Genes (total)                     :: 2,776
            CDSs (total)                      :: 2,750
            Genes (coding)                    :: 2,714
            CDSs (with protein)               :: 2,714
            Genes (RNA)                       :: 26
            tRNAs                             :: 24
            ncRNAs                            :: 2
            Pseudo Genes (total)              :: 36
            CDSs (without protein)            :: 36
            Pseudo Genes (ambiguous residues) :: 0 of 36
            Pseudo Genes (frameshifted)       :: 2 of 36
            Pseudo Genes (incomplete)         :: 32 of 36
            Pseudo Genes (internal stop)      :: 2 of 36
            ##Genome-Annotation-Data-END##
FEATURES             Location/Qualifiers
     source          1..2127
                     /organism="Micropruina sp."
                     /mol_type="genomic DNA"
                     /submitter_seqid="NODE_21052_length_2127_cov_3.575290"
                     /isolate="AMR_MDS_2195"
                     /isolation_source="Activated sludge of municipal
                     wastewater treatment plants"
                     /db_xref="taxon:2737536"
                     /environmental_sample
                     /geo_loc_name="Canada"
                     /lat_lon="49.25 N 123.1 W"
                     /collection_date="2015-06-16"
                     /metagenome_source="wastewater metagenome"
                     /note="metagenomic"
     gene            <1..846
                     /locus_tag="PLF56_13460"
     CDS             <1..846
                     /locus_tag="PLF56_13460"
                     /inference="COORDINATES: protein motif:HMM:NF017984.2"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_function: GO:0019104 - DNA N-glycosylase activity
                     [Evidence IEA];
                     GO_process: GO:0036297 - interstrand cross-link repair
                     [Evidence IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="crosslink repair DNA glycosylase YcaQ family
                     protein"
                     /protein_id="HOQ54606.1"
                     /translation="RAIGDLPLYQAYFRRPLRYQQTHDWLAANDGFRRDVLARLRADG
                     PLRTGEIADTASASWRSTGWTNNRNVTQLLELLVRLGEVAIAGREGRERCWDLAERVY
                     PADLPVIDLETAQQRRNLRRLQALGIARARQTEVPLERMDVGDAGEPAVVEGVAGEWR
                     VDPLGLDELAVPFEGRTALLSPFDRLVFDRVRAQELFGFEYVLEMYKPAASRRWGYFT
                     LPILHGEDLIGKLDAKADRKAGALRVFAVHRDGEWSRSAVADVHAEIRSLAEWQGLEW
                     DGAPG"
     gene            complement(1391..>2127)
                     /locus_tag="PLF56_13465"
     CDS             complement(1391..>2127)
                     /locus_tag="PLF56_13465"
                     /inference="COORDINATES: protein motif:HMM:NF013715.2"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_component: GO:0016020 - membrane [Evidence IEA];
                     GO_function: GO:0022857 - transmembrane transporter
                     activity [Evidence IEA];
                     GO_process: GO:0055085 - transmembrane transport [Evidence
                     IEA]"
                     /codon_start=3
                     /transl_table=11
                     /product="Nramp family divalent metal transporter"
                     /protein_id="HOQ54607.1"
                     /translation="GALFWAPPDWRQTVGGLIPGVPDDDAWPLIAAMLGATVMPHAIY
                     LHSALAIDRHRPHGRLAVPLRRLLNVQKLDVLLALLLSGSVNIGMLLLAASALRGEVG
                     DTIEAAFATITTRVGSVPALVFAIGLLASGVGSAVVGTHAGSRILKDLIGLPVAPTVR
                     RIITVGPAVLLLTLGVSPTEVLVWSQVVLSFGVALAAAPLADFTGDRRLMGEHVDAPW
                     LRVVNWTIVAAIVALNLVTIQWALTN"
BASE COUNT          336 a          716 c          779 g          296 t
ORIGIN      
        1 cgggcgatcg gcgacctgcc gctgtatcag gcgtacttcc gccggccgct gaggtaccag
       61 cagacccacg actggctggc ggccaacgac ggctttcggc gcgatgtgct ggcccggctg
      121 cgcgcggacg gcccgctgcg gaccggtgag atcgccgaca cggcgtccgc gtcgtggcgg
      181 tcgaccggct ggacgaacaa ccgcaacgtc acccagctac tggagctgct ggtgcgactg
      241 ggtgaggtgg cgatcgccgg acgcgagggt cgtgagcggt gctgggacct ggccgagagg
      301 gtgtatccgg ccgacctgcc ggtgatcgac ctcgagaccg cgcagcagcg acgcaacctg
      361 cgccgacttc aggcgctggg catcgcgcgg gccaggcaga ccgaggtgcc gctggaacgc
      421 atggacgtcg gcgatgcggg cgagcccgcc gtggtcgagg gtgtggcggg ggagtggcgg
      481 gtcgatccgc tcggcctgga cgaactggcc gtcccgttcg agggtcgcac cgccctgctg
      541 tcgcccttcg atcgactggt gttcgaccgc gtccgcgcgc aggagttgtt cggcttcgag
      601 tacgtgctcg aaatgtacaa gccggctgcc agtcggcgct gggggtactt cacgctgccg
      661 atcctgcacg gcgaggacct gatcggaaag ctggacgcca aggccgaccg caaggccggg
      721 gcgctgcggg tgttcgcggt tcaccgcgac ggcgagtggt cacgttcggc cgtggcggac
      781 gtgcatgccg agatccggtc cctggcggaa tggcaggggt tggagtggga cggcgcgccg
      841 gggtgaactg cttggtgcgg gcagagatcc cggctgtgag tctttgggat tacagagcgt
      901 ccggggggat gccggagcgt ccgcggggtg acggagtgcc cgcggggtga cggagtgccc
      961 ctcgtcatcc cggcgaacgc cgggatctcg ttcagctcga cagctacttg caggtcaggt
     1021 caggtcaggc ggcacgtcgg ggccgatcag agcccgccgg tcgtccgccc agggacggac
     1081 cggatgtgcg gcacgccacg gccaccagcg actccggcgc caacgggtcc gacaaccggc
     1141 tcagctgctg gctctgctcg ctgcatgagg gtgcacccag gagcgggcac gccctagcgg
     1201 accggcccga atccgacccg cccggcgggc cgtggtgccg cgccgcgtgg tgcttggcgc
     1261 ctaccccggc gccatggaat tccgcagcgc cgtgggtgat gtgctcgatc ccaccctggg
     1321 accgtccgca ctgcgccagt tcggcgtcgc gaagcgcgcc ggagcatcga gtccgctcat
     1381 gccaagcggc tcagttcgtc agtgcccact ggatcgtgac gaggttgagg gcgacgatcg
     1441 cagcgacgat cgtccagttc accacccgca gccacggtgc gtccacgtgc tcccccatca
     1501 gccggcgatc accggtgaag tcggccaggg gagcggccgc cagcgccacg ccgaacgaca
     1561 gcaccacctg cgaccacacc aggacctcgg tcggcgaaac gcccagggtg agcagcagca
     1621 cggccggccc gacggtgatg atgcgccgca cggtgggcgc cacgggtagg ccgatcaggt
     1681 ccttcagaat gcgcgagccc gcgtgggtgc cgacgacggc cgatccgacg cccgaggcca
     1741 gtagcccgat cgcgaacacc agcgccggca cgctgccgac ccgcgtggtg atggtggcga
     1801 acgccgcctc gatggtgtca ccgacttcac cacgcagagc cgacgcggcc aacagcagca
     1861 tgccgatgtt caccgacccc gacagcagca gcgccagtag cacgtcgagt ttctgcacgt
     1921 tcagcagacg tcgaagcggc accgccagtc gtccatgggg tcgatgccgg tcgatggcca
     1981 gcgccgaatg cagatagatg gcgtgcggca tcacggtggc gcccagcatg gcggcgatca
     2041 gcggccacgc gtcgtcgtcc ggaacacctg gaatcagacc gccgacggtc tgccgccaat
     2101 ccggcggcgc ccagaacagg gcaccga
//
LOCUS       DAOSUG010000512         2116 bp    DNA     linear   ENV 10-JUN-2024
DEFINITION  MAG TPA_asm: Micropruina sp. isolate AMR_MDS_2195
            NODE_21202_length_2116_cov_4.874818, whole genome shotgun sequence.
ACCESSION   DAOSUG010000512 DAOSUG010000000
VERSION     DAOSUG010000512.1
DBLINK      BioProject: PRJNA925477
            BioSample: SAMN32864262
            Sequence Read Archive: SRR4236673
KEYWORDS    WGS; ENV; Metagenome Assembled Genome; MAG; Third Party Data; TPA;
            TPA:assembly.
SOURCE      Micropruina sp. (wastewater metagenome)
  ORGANISM  Micropruina sp.
            Bacteria; Bacillati; Actinomycetota; Actinomycetes;
            Propionibacteriales; Nocardioidaceae; Micropruina.
REFERENCE   1  (bases 1 to 2116)
  AUTHORS   Abdulkadir,N., Saraiva,J.P., Zhang,J., Stolte,S., Gillor,O.,
            Harms,H. and Rocha,U.
  TITLE     Genome-centric analyses of 165 metagenomes show that mobile genetic
            elements are crucial for the transmission of antimicrobial
            resistance genes to pathogens in activated sludge and wastewater
  JOURNAL   Microbiol Spectr 12 (3), e0291823 (2024)
   PUBMED   38289113
REFERENCE   2  (bases 1 to 2116)
  AUTHORS   Abdulkadir,N.
  TITLE     Direct Submission
  JOURNAL   Submitted (24-MAR-2023) Environmental Microbiology, Helmholtz
            Center for Environmental Research -UFZ, Permoserstr 15, Leipzig
            04318, Germany
COMMENT     The annotation was added by the NCBI Prokaryotic Genome Annotation
            Pipeline (PGAP). Information about PGAP can be found here:
            https://www.ncbi.nlm.nih.gov/genome/annotation_prok/
            
            ##Genome-Assembly-Data-START##
            Assembly Method        :: SPAdes v. 3.11.1
            Genome Representation  :: Full
            Expected Final Version :: Yes
            Genome Coverage        :: Not Applicable
            Sequencing Technology  :: Illumina MiSeq
            ##Genome-Assembly-Data-END##
            
            ##Genome-Annotation-Data-START##
            Annotation Provider               :: NCBI
            Annotation Date                   :: 05/04/2023 04:57:10
            Annotation Pipeline               :: NCBI Prokaryotic Genome
                                                 Annotation Pipeline (PGAP)
            Annotation Method                 :: Best-placed reference protein
                                                 set; GeneMarkS-2+
            Annotation Software revision      :: 6.5
            Features Annotated                :: Gene; CDS; rRNA; tRNA; ncRNA
            Genes (total)                     :: 2,776
            CDSs (total)                      :: 2,750
            Genes (coding)                    :: 2,714
            CDSs (with protein)               :: 2,714
            Genes (RNA)                       :: 26
            tRNAs                             :: 24
            ncRNAs                            :: 2
            Pseudo Genes (total)              :: 36
            CDSs (without protein)            :: 36
            Pseudo Genes (ambiguous residues) :: 0 of 36
            Pseudo Genes (frameshifted)       :: 2 of 36
            Pseudo Genes (incomplete)         :: 32 of 36
            Pseudo Genes (internal stop)      :: 2 of 36
            ##Genome-Annotation-Data-END##
FEATURES             Location/Qualifiers
     source          1..2116
                     /organism="Micropruina sp."
                     /mol_type="genomic DNA"
                     /submitter_seqid="NODE_21202_length_2116_cov_4.874818"
                     /isolate="AMR_MDS_2195"
                     /isolation_source="Activated sludge of municipal
                     wastewater treatment plants"
                     /db_xref="taxon:2737536"
                     /environmental_sample
                     /geo_loc_name="Canada"
                     /lat_lon="49.25 N 123.1 W"
                     /collection_date="2015-06-16"
                     /metagenome_source="wastewater metagenome"
                     /note="metagenomic"
     gene            complement(215..1897)
                     /locus_tag="PLF56_13470"
     CDS             complement(215..1897)
                     /locus_tag="PLF56_13470"
                     /EC_number="3.1.-.-"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_013161320.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_function: GO:0003723 - RNA binding [Evidence IEA];
                     GO_function: GO:0004540 - ribonuclease activity [Evidence
                     IEA];
                     GO_function: GO:0008270 - zinc ion binding [Evidence IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="ribonuclease J"
                     /protein_id="HOQ54608.1"
                     /translation="MRDSLKTPPKLPQDTLRVTPLGGLGDVGRNSTSFEINGQLLMID
                     CGVLFPSDDHPGVDLILPGLHLIEDRLDDVAALVLTHGHEDHIGAVPYLLRRRPNIPV
                     LGSRLTLALLREKLKEHRLRDVDLRVVSEGTRIKVGEFDLEFLAVNHSIPDALAIALR
                     TAAGLVLHTGDFKMDQLPLDHRLTDLRGFARLGEEGVDLFMADSTNAETPGFTTHERD
                     IEPAMERVFGSAKQKLIVACFASHVHRVQQVLDAAVRHGRKVVYVGRSMVRNMTVAQE
                     LGYLRVPPNTLIELKQLENYPDDKVVLVSTGSQGEPLSALSRISNHEHPVVTAGPGDV
                     VLLASSLIPGNENSVYRVINGLARNGVTVVHKGNALVHVSGHASAGELLYCYNLIKPR
                     NVLPVHGEIRHLIANADLAVATGVPRDRALVIEDGGVVDLAKGRAKVVGRLDCGYIFV
                     DGSTVGDIGDAELSDRKILGSDGFITVVVVVNLHAGRLVSGPDIHARGFVEDDSVFDG
                     IKSEIAKAVEQAMADGSEDVRGLQQVVRRTVGRWVSNTYRRRPMILPVVVSA"
BASE COUNT          336 a          738 c          704 g          338 t
ORIGIN      
        1 ggcccttggc gcagatgtca cacacggcag ccactggtaa ctccttgatt gcttgagatc
       61 gttccctaca gggaccgtcc aacaataccc gagagcccgc agccagacct aatcgagacc
      121 ggtcccgcgc gggccgcagg tcgagctggg gcagtccagc tcacccgatg caatcgacgc
      181 cggtcccgcg cgggctgcag gcttgacgcg acgctcaggc cgagaccacc accggcagaa
      241 tcatcggacg gcgccggtag gtgttgctga cccatcgtcc gacggtgcgg cgcaccacct
      301 gctgcagccc acggacgtcc tcgctgccgt ccgccatcgc ctgctcgacc gccttggcga
      361 tctcgctctt gatgccgtcg aacaccgaat cgtcctcgac gaagccgcgg gcgtgaatgt
      421 cggggccgct gaccagccgg ccggcatgca ggttcaccac caccaccacg gtgatgaatc
      481 cgtccgagcc gaggatcttg cgatcgctca gttcggcatc gccgatgtca cccacggtgg
      541 agccgtccac gaagatgtag ccgcagtcga gccgtccgac caccttggcg cggcccttgg
      601 ccaggtcgac caccccgccg tcctcgatca ccagggcacg atcacgcggc acgccggtgg
      661 caacggccag gtcggcgttg gcgatcaggt gccggatctc accgtgcacc ggcaggacgt
      721 tcctgggctt gatcaggttg tagcagtaga gcaactcccc cgccgaggcg tggcccgaca
      781 cgtgcaccag cgcattgccc ttgtgcacca cggtgacacc gttgcgggcg agcccgttga
      841 tcacccggta gaccgagttc tcgttgcccg ggatcaggga cgacgcgagc agcaccacat
      901 caccggggcc ggcggtgacc accgggtgct cgtggttcga gatgcgtgac agtgccgaga
      961 gcggctcgcc ctgcgacccg gtcgacacca gcaccacctt gtcgtcgggg tagttctcga
     1021 gttgcttgag ttcgatcagg gtgttcggcg gcacccgaag gtacccgagt tcctgcgcga
     1081 cggtcatgtt gcgcaccatg gagcgtccga cgtagaccac cttgcgcccg tgccgcacgg
     1141 ccgcatcgag cacctgctgc acgcggtgca cgtgcgaggc gaagcacgcc acgatcagtt
     1201 tctgcttggc cgagccgaat acccgctcca tggcgggttc gatgtcgcgc tcgtgggtgg
     1261 tgaagccggg ggtctcggcg ttggtggagt cggccatgaa caggtcgacc ccctcttcgc
     1321 cgagccgcgc gaagccgcgc aggtcggtca gccggtgatc cagcggcagt tggtccatct
     1381 tgaagtcgcc ggtgtgcagc accaggccgg cggcggtgcg cagggcgatc gccagggcgt
     1441 ccggaatgga gtggttgacg gccagaaact ccaggtcgaa ctcgcccacc ttgatgcggg
     1501 tgccctcact gaccacccgc aggtcgacgt cgcgcaggcg gtgttccttc agcttttcgc
     1561 gcagcaacgc aagggtgagc cgtgagccca gcaccggaat gttcggacgc cgccgcaaca
     1621 ggtacggcac ggcgccgatg tgatcttcgt ggccgtgggt gagcaccagg gccgcgacgt
     1681 cgtccagccg gtcttcaatc aggtgcaggc cgggcaggat caggtccacc cccggatggt
     1741 cgtcggaggg gaacagcacc ccgcagtcga tcatcaacag ttggccgttg atctcgaagc
     1801 tggtgctgtt gcggcccacg tcgcccagac ccccgagcgg ggtcactcgc agggtgtctt
     1861 ggggcagctt ggggggagtc ttcagcgaat cacgcacgct cccaccctag tggtgccccg
     1921 cgcgccgccg caccggcgtg cccgccgtcg ggctcggcca cgtcgccggc ccgtgccggc
     1981 ggtgcacgtc gccgccatgc cgggacgccc cgcaccggcc cgtgcggcag gcgcaggtcg
     2041 ctagggcgtg tcgcataacc ccgaccgctc tgcggcgcac ccagtgcacc cgctcgctgc
     2101 gtcatccgcg gcggta
//
LOCUS       DAOSUG010000513         2107 bp    DNA     linear   ENV 10-JUN-2024
DEFINITION  MAG TPA_asm: Micropruina sp. isolate AMR_MDS_2195
            NODE_21337_length_2107_cov_3.558480, whole genome shotgun sequence.
ACCESSION   DAOSUG010000513 DAOSUG010000000
VERSION     DAOSUG010000513.1
DBLINK      BioProject: PRJNA925477
            BioSample: SAMN32864262
            Sequence Read Archive: SRR4236673
KEYWORDS    WGS; ENV; Metagenome Assembled Genome; MAG; Third Party Data; TPA;
            TPA:assembly.
SOURCE      Micropruina sp. (wastewater metagenome)
  ORGANISM  Micropruina sp.
            Bacteria; Bacillati; Actinomycetota; Actinomycetes;
            Propionibacteriales; Nocardioidaceae; Micropruina.
REFERENCE   1  (bases 1 to 2107)
  AUTHORS   Abdulkadir,N., Saraiva,J.P., Zhang,J., Stolte,S., Gillor,O.,
            Harms,H. and Rocha,U.
  TITLE     Genome-centric analyses of 165 metagenomes show that mobile genetic
            elements are crucial for the transmission of antimicrobial
            resistance genes to pathogens in activated sludge and wastewater
  JOURNAL   Microbiol Spectr 12 (3), e0291823 (2024)
   PUBMED   38289113
REFERENCE   2  (bases 1 to 2107)
  AUTHORS   Abdulkadir,N.
  TITLE     Direct Submission
  JOURNAL   Submitted (24-MAR-2023) Environmental Microbiology, Helmholtz
            Center for Environmental Research -UFZ, Permoserstr 15, Leipzig
            04318, Germany
COMMENT     The annotation was added by the NCBI Prokaryotic Genome Annotation
            Pipeline (PGAP). Information about PGAP can be found here:
            https://www.ncbi.nlm.nih.gov/genome/annotation_prok/
            
            ##Genome-Assembly-Data-START##
            Assembly Method        :: SPAdes v. 3.11.1
            Genome Representation  :: Full
            Expected Final Version :: Yes
            Genome Coverage        :: Not Applicable
            Sequencing Technology  :: Illumina MiSeq
            ##Genome-Assembly-Data-END##
            
            ##Genome-Annotation-Data-START##
            Annotation Provider               :: NCBI
            Annotation Date                   :: 05/04/2023 04:57:10
            Annotation Pipeline               :: NCBI Prokaryotic Genome
                                                 Annotation Pipeline (PGAP)
            Annotation Method                 :: Best-placed reference protein
                                                 set; GeneMarkS-2+
            Annotation Software revision      :: 6.5
            Features Annotated                :: Gene; CDS; rRNA; tRNA; ncRNA
            Genes (total)                     :: 2,776
            CDSs (total)                      :: 2,750
            Genes (coding)                    :: 2,714
            CDSs (with protein)               :: 2,714
            Genes (RNA)                       :: 26
            tRNAs                             :: 24
            ncRNAs                            :: 2
            Pseudo Genes (total)              :: 36
            CDSs (without protein)            :: 36
            Pseudo Genes (ambiguous residues) :: 0 of 36
            Pseudo Genes (frameshifted)       :: 2 of 36
            Pseudo Genes (incomplete)         :: 32 of 36
            Pseudo Genes (internal stop)      :: 2 of 36
            ##Genome-Annotation-Data-END##
FEATURES             Location/Qualifiers
     source          1..2107
                     /organism="Micropruina sp."
                     /mol_type="genomic DNA"
                     /submitter_seqid="NODE_21337_length_2107_cov_3.558480"
                     /isolate="AMR_MDS_2195"
                     /isolation_source="Activated sludge of municipal
                     wastewater treatment plants"
                     /db_xref="taxon:2737536"
                     /environmental_sample
                     /geo_loc_name="Canada"
                     /lat_lon="49.25 N 123.1 W"
                     /collection_date="2015-06-16"
                     /metagenome_source="wastewater metagenome"
                     /note="metagenomic"
     gene            complement(<1..636)
                     /locus_tag="PLF56_13475"
     CDS             complement(<1..636)
                     /locus_tag="PLF56_13475"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_014598935.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="carboxyl transferase domain-containing protein"
                     /protein_id="HOQ54609.1"
                     /translation="MAKPRDMKNRVDELLDKRLTTRAGGGEKRIAKQHAAGKLTARER
                     IDALIDEGSFQEIGLFRRNRTVTFGMDKADMPADGVVTGTGAVLGRPVHIASQDFTVA
                     GGSAGETHSKKVVEMMESALKCGTPFVFINDSGGARVQEGIDSLSGYSQVFFANVLLS
                     GAVPQISIIAGPCAGGAAYSPALTDFIIQTRKAHMFITGPNVIEQVTGEKVT"
     gene            complement(641..>2107)
                     /locus_tag="PLF56_13480"
     CDS             complement(641..>2107)
                     /locus_tag="PLF56_13480"
                     /EC_number="2.1.3.1"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_002550269.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="methylmalonyl-CoA carboxytransferase subunit 5S"
                     /protein_id="HOQ54610.1"
                     /translation="LVLRDGHQSLLATRMAMEDMVGACADIDAAGYWSVECWGGATYD
                     SCIRFLNEDPWERLRTFRKLLPNSRLQMLLRGQNLLGYRHYEDEVVERFVDKSAENGM
                     DVFRVFDALNDPRNMAHAMQAVKKTGKHAQGTICYTISPLHTVEGYVHLAGQLLDMGA
                     ESIALKDMAALLQPQPAYDIVRGIKETYGEDVQINVHCHATTGVTLVSLMKAIEAGAD
                     VVDTSISSMSLGPGHNPTESLVAMLEGTPYTTNLDADRLIKIRDHFAKVRPKYAEFES
                     ATLVDTDIFSSQIPGGMLSNMESQLKAQGAGDRIKEVMAEVPLVKADSGHPPLVTPSS
                     QIVGTQAVFNVLMGKYKVMTGEFADLMLGYYGECIGERNPEVVAIAEKQAKKEPITVR
                     PADLLPPEWNNLSAEAAKLDGFDGTDEDVLTYAMFPGVAPTFFSTRAEGPKNVGKTEA
                     QVKAEADAKSDSGTAVTGPIKYKVSLGGREHSVTVERA"
BASE COUNT          321 a          694 c          684 g          408 t
ORIGIN      
        1 ggtcaccttc tcgccggtga cctgctcgat cacgttcggc ccggtgatga acatgtgggc
       61 cttgcgcgtc tggatgatga agtcggtcag cgccgggctg tacgccgcgc caccggcgca
      121 cgggccggcg atgatcgaga tctgcggcac cgcgcccgac agcagcacgt tggcgaagaa
      181 cacctggctg tagcccgaca gcgagtcgat gccctcttgg acgcgcgcgc cgccggaatc
      241 gttgatgaag acgaacgggg tgccgcactt gagcgcggac tccatcatct cgacgacctt
      301 cttggagtgc gtctcgcctg cggagccacc ggcgacggtg aagtcctggc tggcgatgtg
      361 caccggacgt ccgagcaccg cgcccgtgcc ggtcaccaca ccgtccgcag gcatgtcggc
      421 cttgtccatg ccgaacgtca cggtgcggtt gcgccggaac agcccgatct cttggaagga
      481 gccctcatcg atcagtgcgt cgatgcgttc gcgtgcggtc agtttgcccg ccgcgtgctg
      541 cttcgcgatg cgcttctcgc cgccaccggc ccgggtggtg agccgcttgt cgagcagctc
      601 gtcgacccgg ttcttcatgt ctcttggttt cgccatgccc tcacgccctc tcgacggtga
      661 cgctgtgctc gcgtccgccc aaactgacct tgtacttgat cggcccggtg accgccgtac
      721 ccgagtcgga cttcgcgtcc gcctcggcct tcacctgcgc ctcggtcttg ccgacattct
      781 tcgggccctc ggcgcgagtg ctgaagaagg tgggggcgac gcccgggaac atggcgtagg
      841 tgagcacgtc ctcatcggtg ccgtcgaagc cgtcaagctt ggcggcctcg gccgacaggt
      901 tgttccactc gggcggcagc aggtcggccg gacgaaccgt gatcggctct ttcttggcct
      961 gcttctcggc gatcgcgacg acctcggggt tgcgctcgcc gatgcattcg ccgtagtagc
     1021 cgagcatcag gtcggcgaac tcgccggtca tcaccttgta cttgcccatc agcacgttga
     1081 acacggcctg ggtgccgacg atctggctgg acggggtgac cagcggcggg tgaccggaat
     1141 cggccttcac cagcggcacc tcggccatca cttccttgat ccggtcgccg gcgccctgag
     1201 ccttcagctg gctctccatg ttcgagagca tgccgccggg gatctgggag ctgaagatgt
     1261 cggtgtccac cagcgtggcc gactcgaatt cggcgtactt cgggcgaacc ttggcgaagt
     1321 ggtcgcggat cttgatcagc cgatcggcgt ccaggttcgt ggtgtacggg gtgccttcca
     1381 gcatcgccac cagcgactcg gtcgggttgt ggcccgggcc gagcgacatc gagctgatcg
     1441 aggtgtcgac cacgtccgca ccggcttcga tcgccttcat cagcgaaacg agggtcacgc
     1501 cggtggtggc gtggcagtgg acgttgatct ggacgtcctc gccgtatgtc tccttgatac
     1561 cgcggacgat gtcgtaggcg ggctgcggct gcagcagcgc ggccatgtcc ttcagcgcga
     1621 tcgactcggc acccatgtcg agcaactggc cggcgaggtg cacgtagccc tcgacggtgt
     1681 gcagcggcga gatggtgtag cagatcgtgc cctgcgcgtg cttgccggtc ttcttgaccg
     1741 cctgcatcgc gtgcgccatg ttgcgcggat cgttcagcgc gtcgaagaca cggaacacat
     1801 ccatgccgtt ctcggcggac ttgtccacga accgctcgac cacttcgtcc tcgtagtggc
     1861 ggtagccgag caggttctgg ccacgcagca gcatctgcag gcgactgttc ggcagcagct
     1921 tgcggaaggt ccgcaggcgc tcccacggat cctcattcag gaaacggata caactgtcat
     1981 aggtcgctcc gccccaacac tcgactgacc aatagccggc tgcgtcgatg tcggcgcaag
     2041 caccgaccat atcttccatg gccatgcggg tggccaacag gctctgatgg ccatcgcgta
     2101 gcaccag
//
LOCUS       DAOSUG010000514         2084 bp    DNA     linear   ENV 10-JUN-2024
DEFINITION  MAG TPA_asm: Micropruina sp. isolate AMR_MDS_2195
            NODE_21675_length_2084_cov_5.611138, whole genome shotgun sequence.
ACCESSION   DAOSUG010000514 DAOSUG010000000
VERSION     DAOSUG010000514.1
DBLINK      BioProject: PRJNA925477
            BioSample: SAMN32864262
            Sequence Read Archive: SRR4236673
KEYWORDS    WGS; ENV; Metagenome Assembled Genome; MAG; Third Party Data; TPA;
            TPA:assembly.
SOURCE      Micropruina sp. (wastewater metagenome)
  ORGANISM  Micropruina sp.
            Bacteria; Bacillati; Actinomycetota; Actinomycetes;
            Propionibacteriales; Nocardioidaceae; Micropruina.
REFERENCE   1  (bases 1 to 2084)
  AUTHORS   Abdulkadir,N., Saraiva,J.P., Zhang,J., Stolte,S., Gillor,O.,
            Harms,H. and Rocha,U.
  TITLE     Genome-centric analyses of 165 metagenomes show that mobile genetic
            elements are crucial for the transmission of antimicrobial
            resistance genes to pathogens in activated sludge and wastewater
  JOURNAL   Microbiol Spectr 12 (3), e0291823 (2024)
   PUBMED   38289113
REFERENCE   2  (bases 1 to 2084)
  AUTHORS   Abdulkadir,N.
  TITLE     Direct Submission
  JOURNAL   Submitted (24-MAR-2023) Environmental Microbiology, Helmholtz
            Center for Environmental Research -UFZ, Permoserstr 15, Leipzig
            04318, Germany
COMMENT     The annotation was added by the NCBI Prokaryotic Genome Annotation
            Pipeline (PGAP). Information about PGAP can be found here:
            https://www.ncbi.nlm.nih.gov/genome/annotation_prok/
            
            ##Genome-Assembly-Data-START##
            Assembly Method        :: SPAdes v. 3.11.1
            Genome Representation  :: Full
            Expected Final Version :: Yes
            Genome Coverage        :: Not Applicable
            Sequencing Technology  :: Illumina MiSeq
            ##Genome-Assembly-Data-END##
            
            ##Genome-Annotation-Data-START##
            Annotation Provider               :: NCBI
            Annotation Date                   :: 05/04/2023 04:57:10
            Annotation Pipeline               :: NCBI Prokaryotic Genome
                                                 Annotation Pipeline (PGAP)
            Annotation Method                 :: Best-placed reference protein
                                                 set; GeneMarkS-2+
            Annotation Software revision      :: 6.5
            Features Annotated                :: Gene; CDS; rRNA; tRNA; ncRNA
            Genes (total)                     :: 2,776
            CDSs (total)                      :: 2,750
            Genes (coding)                    :: 2,714
            CDSs (with protein)               :: 2,714
            Genes (RNA)                       :: 26
            tRNAs                             :: 24
            ncRNAs                            :: 2
            Pseudo Genes (total)              :: 36
            CDSs (without protein)            :: 36
            Pseudo Genes (ambiguous residues) :: 0 of 36
            Pseudo Genes (frameshifted)       :: 2 of 36
            Pseudo Genes (incomplete)         :: 32 of 36
            Pseudo Genes (internal stop)      :: 2 of 36
            ##Genome-Annotation-Data-END##
FEATURES             Location/Qualifiers
     source          1..2084
                     /organism="Micropruina sp."
                     /mol_type="genomic DNA"
                     /submitter_seqid="NODE_21675_length_2084_cov_5.611138"
                     /isolate="AMR_MDS_2195"
                     /isolation_source="Activated sludge of municipal
                     wastewater treatment plants"
                     /db_xref="taxon:2737536"
                     /environmental_sample
                     /geo_loc_name="Canada"
                     /lat_lon="49.25 N 123.1 W"
                     /collection_date="2015-06-16"
                     /metagenome_source="wastewater metagenome"
                     /note="metagenomic"
     gene            complement(<1..872)
                     /locus_tag="PLF56_13485"
     CDS             complement(<1..872)
                     /locus_tag="PLF56_13485"
                     /EC_number="3.5.99.7"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_012920144.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_function: GO:0008660 -
                     1-aminocyclopropane-1-carboxylate deaminase activity
                     [Evidence IEA];
                     GO_process: GO:0042217 - 1-aminocyclopropane-1-carboxylate
                     catabolic process [Evidence IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="1-aminocyclopropane-1-carboxylate deaminase"
                     /protein_id="HOQ54611.1"
                     /translation="MPIADFPRHPLTFGPSPVHHLKRLSQHLGGAQVWAKREDVNSGL
                     AFGGNKVRKLEYIVPDVLATGADTLVSIGGYQSNHTRQVAAVAAHLGLKCRLVQEKWV
                     PWDDPVNDKVGNILLSRMMGADSRLDPAGFDIGIRDSWKDALAEVEAAGGTPYPIPAG
                     ASEHPLGGLGFANWAFEVAEQERQQGVFFDTIIVCTVTGSTHAGMIAGFAALEDLTGI
                     KRRVIGIDASATLAKTRDQVARIARHTAELIELGRELRDDEITVLEGWAGELYGIPVE
                     STMDAIALGAQLEA"
     gene            complement(977..1669)
                     /locus_tag="PLF56_13490"
     CDS             complement(977..1669)
                     /locus_tag="PLF56_13490"
                     /inference="COORDINATES: protein motif:HMM:NF022655.2"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="LmeA family phospholipid-binding protein"
                     /protein_id="HOQ54612.1"
                     /translation="MKAIRRILIAVLVLAVVLAGAEFGVRALVQSQAQQALTGSDLDL
                     EQPTMTLGGGSVLAALAQGRFVDVSGTAASATVQFEQHAVPVTAITYQASDIRLASTS
                     EAVIGRADLAGTLGYAALSEVAGLPIAYGDDGRVLVTYTVDILGLNSLKIGISAVPQL
                     NVATQQVDLEQSRIDVAGIAIDESLSQQIIERVVKPISLAANENTQVTAITVHPEGLV
                     VDLTATDLPVRR"
     gene            complement(1695..>2084)
                     /locus_tag="PLF56_13495"
     CDS             complement(1695..>2084)
                     /locus_tag="PLF56_13495"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_004808670.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_function: GO:0008839 - 4-hydroxy-tetrahydrodipicolinate
                     reductase [Evidence IEA];
                     GO_process: GO:0009089 - lysine biosynthetic process via
                     diaminopimelate [Evidence IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="dihydrodipicolinate reductase C-terminal
                     domain-containing protein"
                     /protein_id="HOQ54613.1"
                     /translation="QAAAFFESVEVIELHHPNKADAPSGTSAATADRIAAARTAAGLG
                     PVPDATTSSLPGARGAEVAGVHVHSVRLRGLIAHQEVLFGGEGETLTIRHDSLDRVSF
                     MPGVIAAVRAVRERPGLTVGIEAILGL"
BASE COUNT          307 a          725 c          720 g          332 t
ORIGIN      
        1 atcgcttcca gctgcgcacc gagggcgatc gcgtccatcg tggactccac cggaatgccg
       61 tacagctcgc ccgcccaccc ttccagcacg gtgatctcgt cgtcgcgcag ctcgcggccc
      121 agttcgatca gttcggcagt gtggcgggcg atccgggcga cctggtcgcg ggtcttggcc
      181 agcgtggccg acgcgtcgat tccgatcacc cgacgcttga tgccggtcag gtcctccagc
      241 gccgcgaatc cggcgatcat gccggcgtgg gtcgagccgg tcaccgtgca cacgatgatc
      301 gtgtcgaaga acacgccttg ctgccgctcc tgctcggcca cttcgaaggc ccagttggcg
      361 aagccgagcc cacccagcgg atgctccgac gcgcccgccg ggatcgggta gggcgtgccc
      421 cccgcggcct ccacctcggc cagggcgtcc ttccacgagt cgcggatgcc gatgtcgaag
      481 cccgcggggt cgagccggct gtcggcaccc atcatgcgtg acagcagaat gttgccgacc
      541 ttgtcgttca ccgggtcgtc ccagggcacc cacttctcct gcaccagccg gcacttgagc
      601 cccaggtgcg cggcgaccgc ggcgacctgc cgggtgtggt tggactggta acccccgatg
      661 gagaccagcg tgtcggcgcc ggtggcgagc acgtccggaa cgatgtactc gagcttgcgc
      721 accttgttgc cgccgaaggc caggccgctg ttgacgtcct cacgcttggc ccacacctgc
      781 gcaccgccga gatgctgact gagccgcttc aggtgatgga cggggctcgg cccgaacgtg
      841 agcgggtggc ggggaaagtc ggcgatgggc atcgatggtc ctgttctgct cggtggggcg
      901 tggttcatgt gggatgcacg ggcccgggtt gggcggcccg ggttggacgg ggtcagtggt
      961 cacgtggccg gcgcgttcag cggcggacgg gtaggtcggt ggcggtcagg tccacgacca
     1021 ggccttctgg gtggacggtg atggcggtga cctgagtgtt ctcgttcgcc gccagggaga
     1081 tcggcttcac cacccgctcg atgatctgtt gcgagaggct ctcgtcgatc gcgatgcccg
     1141 caacatcgat gcgggactgc tccaggtcca cctgctgcgt cgcaacgttc agttgcggca
     1201 ccgccgaaat gccgatcttg agggagttca gtccgaggat gtcgacggtg taggtgacca
     1261 gcacccgtcc gtcgtcgccg taggcgatcg gcagcccggc cacttcgctg agggcggcat
     1321 agccgagcgt gccggccagg tcggcgcgcc cgatcaccgc ctcgctggta ctggccaggc
     1381 ggatgtccga tgcctgatag gtgatcgcgg tcaccggcac cgcgtgctgc tcgaactgca
     1441 ctgtcgcgga cgccgcggtg cccgacacgt ccacgaaccg gccctgagcc agcgccgcga
     1501 gcaccgaccc gccgccgagg gtcatcgtcg gctgctcgag gtcgaggtcg gagccggtga
     1561 gcgcctgctg agcctgcgac tgcaccagcg cacgcacccc gaactcggcg ccggccagca
     1621 cgacggccag caccagcacc gcgatcagga ttctgcggat ggccttcatc gcgccgcacc
     1681 ggccgctcgt gcgctcacag gccgaggatc gcttcgatgc ccaccgtaag accgggacgt
     1741 tcacgcaccg cacgcaccgc cgcgatcact ccgggcatga acgacacccg gtcgagcgag
     1801 tcgtgccgaa tcgtcagggt ctccccctcg ccgccgaaca acacctcctg gtgggcgatc
     1861 aggccacgca agcgcacgct gtgcacatgc accccggcga cctcggcacc gcgcgcaccg
     1921 ggcagggacg acgtggtggc atcgggcacc gggcccaaac ctgccgctgt gcgagccgcg
     1981 gcgatcctgt cggcggtggc agccgaggtg ccggacgggg cgtccgcctt gttcgggtgg
     2041 tgcagttcga tcacctcgac ggactcgaag aacgccgccg cctg
//
LOCUS       DAOSUG010000515         2077 bp    DNA     linear   ENV 10-JUN-2024
DEFINITION  MAG TPA_asm: Micropruina sp. isolate AMR_MDS_2195
            NODE_21787_length_2077_cov_4.543027, whole genome shotgun sequence.
ACCESSION   DAOSUG010000515 DAOSUG010000000
VERSION     DAOSUG010000515.1
DBLINK      BioProject: PRJNA925477
            BioSample: SAMN32864262
            Sequence Read Archive: SRR4236673
KEYWORDS    WGS; ENV; Metagenome Assembled Genome; MAG; Third Party Data; TPA;
            TPA:assembly.
SOURCE      Micropruina sp. (wastewater metagenome)
  ORGANISM  Micropruina sp.
            Bacteria; Bacillati; Actinomycetota; Actinomycetes;
            Propionibacteriales; Nocardioidaceae; Micropruina.
REFERENCE   1  (bases 1 to 2077)
  AUTHORS   Abdulkadir,N., Saraiva,J.P., Zhang,J., Stolte,S., Gillor,O.,
            Harms,H. and Rocha,U.
  TITLE     Genome-centric analyses of 165 metagenomes show that mobile genetic
            elements are crucial for the transmission of antimicrobial
            resistance genes to pathogens in activated sludge and wastewater
  JOURNAL   Microbiol Spectr 12 (3), e0291823 (2024)
   PUBMED   38289113
REFERENCE   2  (bases 1 to 2077)
  AUTHORS   Abdulkadir,N.
  TITLE     Direct Submission
  JOURNAL   Submitted (24-MAR-2023) Environmental Microbiology, Helmholtz
            Center for Environmental Research -UFZ, Permoserstr 15, Leipzig
            04318, Germany
COMMENT     The annotation was added by the NCBI Prokaryotic Genome Annotation
            Pipeline (PGAP). Information about PGAP can be found here:
            https://www.ncbi.nlm.nih.gov/genome/annotation_prok/
            
            ##Genome-Assembly-Data-START##
            Assembly Method        :: SPAdes v. 3.11.1
            Genome Representation  :: Full
            Expected Final Version :: Yes
            Genome Coverage        :: Not Applicable
            Sequencing Technology  :: Illumina MiSeq
            ##Genome-Assembly-Data-END##
            
            ##Genome-Annotation-Data-START##
            Annotation Provider               :: NCBI
            Annotation Date                   :: 05/04/2023 04:57:10
            Annotation Pipeline               :: NCBI Prokaryotic Genome
                                                 Annotation Pipeline (PGAP)
            Annotation Method                 :: Best-placed reference protein
                                                 set; GeneMarkS-2+
            Annotation Software revision      :: 6.5
            Features Annotated                :: Gene; CDS; rRNA; tRNA; ncRNA
            Genes (total)                     :: 2,776
            CDSs (total)                      :: 2,750
            Genes (coding)                    :: 2,714
            CDSs (with protein)               :: 2,714
            Genes (RNA)                       :: 26
            tRNAs                             :: 24
            ncRNAs                            :: 2
            Pseudo Genes (total)              :: 36
            CDSs (without protein)            :: 36
            Pseudo Genes (ambiguous residues) :: 0 of 36
            Pseudo Genes (frameshifted)       :: 2 of 36
            Pseudo Genes (incomplete)         :: 32 of 36
            Pseudo Genes (internal stop)      :: 2 of 36
            ##Genome-Annotation-Data-END##
FEATURES             Location/Qualifiers
     source          1..2077
                     /organism="Micropruina sp."
                     /mol_type="genomic DNA"
                     /submitter_seqid="NODE_21787_length_2077_cov_4.543027"
                     /isolate="AMR_MDS_2195"
                     /isolation_source="Activated sludge of municipal
                     wastewater treatment plants"
                     /db_xref="taxon:2737536"
                     /environmental_sample
                     /geo_loc_name="Canada"
                     /lat_lon="49.25 N 123.1 W"
                     /collection_date="2015-06-16"
                     /metagenome_source="wastewater metagenome"
                     /note="metagenomic"
     gene            complement(173..>2077)
                     /locus_tag="PLF56_13500"
     CDS             complement(173..>2077)
                     /locus_tag="PLF56_13500"
                     /EC_number="3.6.1.1"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_015011659.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="sodium-translocating pyrophosphatase"
                     /protein_id="HOQ54614.1"
                     /translation="LRIAGGIFTKIADIGSDLMKIVFKIKEDDARNPGVIADCTGDNA
                     GDSVGPSADGFETYGVTGVALVTFIVLAVHNPVVQAQLLCWIFFVRAIMVLASAASYE
                     VNSVITRAKYRNLKTMDFERPLTNLVWITSVVSIAATYFTSYLLIADLGDGTLWWKLS
                     TIISCGTLAGALIPELVKVFTSTSSVHTEEVVKSSHQGGASLNILSGLVAGNFSAFWI
                     GISIIALMAAAFLISSLGVGALMVAPAVFAFGLVAFGFLGMGPVTIAVDSYGPVTDNA
                     QSVYELSMIEDIPGVEDEIRNDYGFTVDWGRAKALLEENDGAGNTFKATAKPVLIGTA
                     VVGATTMIFSIIMGLTDGMTQNLDKLSLLHAPFLLGLITGGAIIYWFTGASIQAVTTG
                     AFRAVEFIKSSINVESLDRASVEDSRKVVEICTQYAQKGMLNIFLGIFFAALSFAFIE
                     PYFFIGYLVSIAIFGLYQAIFMANAGGAWDNAKKIVETDLAAKGTELHDAAVIGDTVG
                     DPYKDTSSVALNPVIKFTTLFGLLAVELAVSMNAQGNHALTWGLAAFFFVAMCVMVWR
                     SFYGMLIDREMLTKADFATLPEAPEPPHVTGVEVELLDDDDDLPPAIASSVGNVRAVM
                     VGIQRDTYDV"
BASE COUNT          372 a          630 c          731 g          344 t
ORIGIN      
        1 tgcggatcgt ggtggtcttg cccgcgccgt tggggccgag cagcgcggtc agttcgcctg
       61 cgccggcgta ccagctgagc cggtcgagca cccggcgcgc gcccaggctg agggtgacct
      121 cgctcagctc gagagggtgg gaggaggtca ccggctcagc atacggcggg ggtcagacgt
      181 cataggtgtc gcgctggatt ccgaccatca ccgcccggac gttgcccacg ctggaggcga
      241 tggcgggggg caggtcgtcg tcatcgtcga gcaactcgac ctcgaccccg gtgacgtgcg
      301 gcggctcagg cgcctcgggc agggtggcga aatcggcctt ggtgagcatc tcgcggtcga
      361 tcagcatgcc gtagaacgag cgccacacca tcacgcacat cgcgacgaag aagaacgcgg
      421 ccagccccca ggtgagggca tggttgccct gcgcgttcat gctcacggcc agctcgacgg
      481 ccagcaggcc gaacagcgtg gtgaacttga tcaccgggtt gagcgcgacc gagctggtgt
      541 ccttgtacgg gtcgccgacg gtgtcgccga tcaccgccgc gtcgtgcaac tcggtgccct
      601 tggcggccag gtcggtctcg acgatcttct tggcgttgtc ccaggcgccg ccagcgttgg
      661 ccatgaagat cgcctggtac aacccgaaga tggcgatgct gaccaggtag ccgatgaaga
      721 agtacggctc gatgaaggcg aacgacaagg cggcgaagaa gatgcccagg aagatgttga
      781 gcatgccctt ctgggcgtac tgggtgcaga tctcgaccac cttgcgcgaa tcctcgaccg
      841 aggcgcggtc cagcgactcg acgttgatcg acgacttgat gaactcgacc gcgcggaacg
      901 cgccggtggt gaccgcctga atggacgccc cggtgaacca gtagatgatt gcgccgccgg
      961 tgatcaggcc gagcaggaac ggggcgtgca gcagcgacag cttgtcgagg ttctgcgtca
     1021 tgccgtcggt caggcccatg atgatcgaga agatcatcgt ggtggcaccg accacggcgg
     1081 tgccgatcag caccggcttg gcggtcgcct tgaacgtgtt gcccgcaccg tcgttctctt
     1141 cgagcagtgc cttggcccgg ccccagtcga cggtgaagcc gtagtcgttg cggatctcgt
     1201 cctcgacgcc cggaatgtct tcgatcatgc tcagttcgta gaccgattgg gcgttgtcgg
     1261 tgaccggccc gtagctgtcg accgcgatgg tgaccgggcc catgccgaga aagccgaatg
     1321 cgaccaggcc gaacgcgaac acggccgggg cgaccatcaa cgcgccgacg cccaggctcg
     1381 agatgaggaa cgcggcggcc atcagggcga tgatcgagat gccgatccag aaggcgctga
     1441 agttgccggc caccaggccc gagagaatgt tgagcgaagc cccgccctgg tgggagctct
     1501 tcaccacctc ttcggtgtgc accgagctgg tcgaggtgaa caccttgacc agttcgggaa
     1561 tcagcgcgcc ggccagggtg ccgcagctga tgatcgtgga cagcttccac cacagggtgc
     1621 cgtcgccgag gtcggcgatc agcaggtacg aggtgaagta ggtggccgcg atcgagacca
     1681 cggacgtgat ccagaccagg ttggtcaggg gacgttcgaa gtccatcgtt ttcaggttgc
     1741 ggtacttggc ccgggtgatc accgagttga cctcgtacga ggcggccgag gccagcacca
     1801 tgatggcgcg gacgaagaag atccagcaca gcagctgggc ctgcacgacc gggttgtgca
     1861 ccgccagcac gatgaaggtg accagcgcga cgccggtgac gccgtaggtc tcgaagccgt
     1921 cggcggacgg gccgaccgag tcgccggcgt tgtcgccggt gcagtcggcg atgacgccgg
     1981 ggttgcgggc gtcgtcctcc ttgatcttga agacgatctt catcaggtcg gagccgatgt
     2041 cggcgatctt ggtgaagatg ccgccggcga tgcgcag
//
LOCUS       DAOSUG010000516         2060 bp    DNA     linear   ENV 10-JUN-2024
DEFINITION  MAG TPA_asm: Micropruina sp. isolate AMR_MDS_2195
            NODE_22035_length_2060_cov_5.119202, whole genome shotgun sequence.
ACCESSION   DAOSUG010000516 DAOSUG010000000
VERSION     DAOSUG010000516.1
DBLINK      BioProject: PRJNA925477
            BioSample: SAMN32864262
            Sequence Read Archive: SRR4236673
KEYWORDS    WGS; ENV; Metagenome Assembled Genome; MAG; Third Party Data; TPA;
            TPA:assembly.
SOURCE      Micropruina sp. (wastewater metagenome)
  ORGANISM  Micropruina sp.
            Bacteria; Bacillati; Actinomycetota; Actinomycetes;
            Propionibacteriales; Nocardioidaceae; Micropruina.
REFERENCE   1  (bases 1 to 2060)
  AUTHORS   Abdulkadir,N., Saraiva,J.P., Zhang,J., Stolte,S., Gillor,O.,
            Harms,H. and Rocha,U.
  TITLE     Genome-centric analyses of 165 metagenomes show that mobile genetic
            elements are crucial for the transmission of antimicrobial
            resistance genes to pathogens in activated sludge and wastewater
  JOURNAL   Microbiol Spectr 12 (3), e0291823 (2024)
   PUBMED   38289113
REFERENCE   2  (bases 1 to 2060)
  AUTHORS   Abdulkadir,N.
  TITLE     Direct Submission
  JOURNAL   Submitted (24-MAR-2023) Environmental Microbiology, Helmholtz
            Center for Environmental Research -UFZ, Permoserstr 15, Leipzig
            04318, Germany
COMMENT     The annotation was added by the NCBI Prokaryotic Genome Annotation
            Pipeline (PGAP). Information about PGAP can be found here:
            https://www.ncbi.nlm.nih.gov/genome/annotation_prok/
            
            ##Genome-Assembly-Data-START##
            Assembly Method        :: SPAdes v. 3.11.1
            Genome Representation  :: Full
            Expected Final Version :: Yes
            Genome Coverage        :: Not Applicable
            Sequencing Technology  :: Illumina MiSeq
            ##Genome-Assembly-Data-END##
            
            ##Genome-Annotation-Data-START##
            Annotation Provider               :: NCBI
            Annotation Date                   :: 05/04/2023 04:57:10
            Annotation Pipeline               :: NCBI Prokaryotic Genome
                                                 Annotation Pipeline (PGAP)
            Annotation Method                 :: Best-placed reference protein
                                                 set; GeneMarkS-2+
            Annotation Software revision      :: 6.5
            Features Annotated                :: Gene; CDS; rRNA; tRNA; ncRNA
            Genes (total)                     :: 2,776
            CDSs (total)                      :: 2,750
            Genes (coding)                    :: 2,714
            CDSs (with protein)               :: 2,714
            Genes (RNA)                       :: 26
            tRNAs                             :: 24
            ncRNAs                            :: 2
            Pseudo Genes (total)              :: 36
            CDSs (without protein)            :: 36
            Pseudo Genes (ambiguous residues) :: 0 of 36
            Pseudo Genes (frameshifted)       :: 2 of 36
            Pseudo Genes (incomplete)         :: 32 of 36
            Pseudo Genes (internal stop)      :: 2 of 36
            ##Genome-Annotation-Data-END##
FEATURES             Location/Qualifiers
     source          1..2060
                     /organism="Micropruina sp."
                     /mol_type="genomic DNA"
                     /submitter_seqid="NODE_22035_length_2060_cov_5.119202"
                     /isolate="AMR_MDS_2195"
                     /isolation_source="Activated sludge of municipal
                     wastewater treatment plants"
                     /db_xref="taxon:2737536"
                     /environmental_sample
                     /geo_loc_name="Canada"
                     /lat_lon="49.25 N 123.1 W"
                     /collection_date="2015-06-16"
                     /metagenome_source="wastewater metagenome"
                     /note="metagenomic"
     gene            complement(30..1361)
                     /locus_tag="PLF56_13505"
     CDS             complement(30..1361)
                     /locus_tag="PLF56_13505"
                     /inference="COORDINATES: protein motif:HMM:NF033550.5"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_function: GO:0004803 - transposase activity [Evidence
                     IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="ISL3 family transposase"
                     /protein_id="HOQ54615.1"
                     /translation="MPDSTSCCRAGGGYCQRCDLLLGLPGLHVVGVQRDDAGLRVEVE
                     SRTPEVMGCPACGVIAHAHGRQRVELIDAPCFTAPVKLWWRKRRWLCPEPMCPVNSFM
                     EQDPDVARPRALLTARATSWAIGQMRRENASVQGMARQLGCAWRTLWRAVKPILEAAA
                     DDETRFAGVTSLGVDEHLWHHVSTKPVDQGGRGPKEFTGMVDLTRDKDGHVRARLLDL
                     VPGRSGEAYKTWLRDRGDTFRKSVEIATLDPFHGYKNAIDDQLEDAVAVLDAFHVVKL
                     GTAAVDEVRRRVQQDIHGHRGRRDDPLYRIRNLLRAGQEHLTDRQKARLETAFTADDR
                     HVEVELAWHCAQQLRSVYHQPSHADGRRVAEKILASFPTCPIPEIARLGRTLNQWRDA
                     FLGYFTTGGANNGGTESINGLIELARRVARGFRDPDNYRLRMLLIGGGLRL"
     gene            1489..>2060
                     /locus_tag="PLF56_13510"
     CDS             1489..>2060
                     /locus_tag="PLF56_13510"
                     /inference="COORDINATES: protein motif:HMM:NF013697.2"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="extracellular solute-binding protein"
                     /protein_id="HOQ54616.1"
                     /translation="MAAFGLSACQTAGNQPAPEDTGSVITQAPVEGATELNLWTFVEL
                     HGKFYTTMAEQWNELHPDKKVQIKVNALPYDDMHNKLQIALTSGTGAPDIADIEAGKF
                     PQFVIGTPALMDLTEAAAPYKDDIVQARLDLFSANGGLYGLDFHVGATVAFYNTELLE
                     AAGIDYTTIKTWDDYRDAGAKYHEATGKYLG"
BASE COUNT          346 a          714 c          678 g          322 t
ORIGIN      
        1 ttgacggctc ttcgtacctg agcgtggggt catagtcgta gaccgccgcc gatgaggagc
       61 attctgaggc ggtagttgtc tgggtcgcgg aagcctctgg cgacgcggcg ggcgagttcg
      121 atcaacccgt tgatggattc ggttccgccg ttgttggcgc cgccggtggt gaagtagccc
      181 aggaacgcgt cgcgccactg gttcagcgtc cggccgaggc gggcgatctc cgggatcgga
      241 cacgtcggga acgatgcgag gatcttctcc gcgaccctgc ggccgtcggc gtggctgggc
      301 tggtggtaga ccgagcggag ctgttgggcg cagtgccagg cgagttcgac ttcgacgtgt
      361 cggtcgtcgg ctgtgaacgc ggtctccagg cgggccttct gccggtcggt aaggtgttcc
      421 tgcccggccc gcagcaggtt ccgaatccga tacaacggat catctctgcg gccgcggtgg
      481 ccgtggatgt cctgctggac ccggcggcgg acctcatcga ccgcggccgt cccgagcttg
      541 accacgtgga aggcgtcgag caccgcaacg gcgtcctcca actggtcgtc gatggcgttc
      601 ttgtagccgt ggaacgggtc cagggtggcg atctcgacgc ttttgcggaa cgtgtcgccg
      661 cggtcacgca gccaggtctt gtaggcctca ccggagcggc ccgggaccag atccagcagc
      721 cgggcccgga cgtgtccgtc cttgtcgcgg gtgagatcga ccatgccggt gaactccttc
      781 gggccgcgtc cgccctggtc gacaggcttg gtggagacgt ggtgccagag gtgttcgtcg
      841 acgccaagcg aggtgacgcc agcgaagcgg gtctcgtcgt cggcagccgc ttcaaggatc
      901 ggcttgaccg ctcgccacag cgtcctccag gcgcagccga gttgccgggc catcccctga
      961 acggaggcgt tctcccgccg catctgcccg atcgcccacg acgtcgcccg agcggtgagc
     1021 aacgctctgg gccgcgccac atcggggtct tgctccatga acgagttcac cggacacatc
     1081 ggctcgggac acagccagcg tcgtttccgc caccacagct tcaccggcgc cgtgaagcac
     1141 ggcgcgtcga tcagctcgac cctctgccgg ccatgcgcat gcgcgatcac gccgcaggcg
     1201 gggcagccca tcacctccgg cgtccgtgac tcgacctcga cacgcagacc cgcgtcgtcg
     1261 cgctggacgc cgaccacgtg gagccccggc aggcccaaca gaaggtcgca gcgctgacag
     1321 tagccgccgc cggcacggca gcaggaagta gaatcgggca tcgtcgaggt ccttgaagat
     1381 caggtggtgt ggtaaccccg attcttgagg gcctcgacgc ccatcacccc gccgacacgc
     1441 ccgccgccgg atccacgctc accccacgct caggtacgaa gagccggcat ggccgcgttc
     1501 gggctgagcg cgtgccagac cgcgggcaac cagcccgctc cggaagacac gggctcggtc
     1561 atcacgcagg cccccgtgga gggagcgacc gagctcaacc tgtggacgtt cgtcgaactg
     1621 cacggcaagt tctacacgac catggccgag cagtggaacg agctgcaccc ggacaagaag
     1681 gtccagatca aggtcaacgc cctcccctac gacgacatgc acaacaagct gcagatcgcc
     1741 ctgacctccg gcaccggcgc ccctgacatc gccgacatcg aggcgggcaa gttcccgcag
     1801 ttcgtcatcg gcaccccggc cctcatggac ctcaccgagg cggcggcgcc gtacaaggac
     1861 gacatcgtcc aggcccgcct cgacctgttc tccgccaacg gcggcctgta cggcctcgac
     1921 ttccacgtcg gcgccaccgt cgccttctac aacaccgaac tgctcgaggc cgccggcatc
     1981 gactacacca ccatcaagac gtgggacgac taccgcgacg cgggagccaa gtaccacgag
     2041 gcgacgggca agtacctcgg
//
LOCUS       DAOSUG010000517         2058 bp    DNA     linear   ENV 10-JUN-2024
DEFINITION  MAG TPA_asm: Micropruina sp. isolate AMR_MDS_2195
            NODE_22069_length_2058_cov_5.232152, whole genome shotgun sequence.
ACCESSION   DAOSUG010000517 DAOSUG010000000
VERSION     DAOSUG010000517.1
DBLINK      BioProject: PRJNA925477
            BioSample: SAMN32864262
            Sequence Read Archive: SRR4236673
KEYWORDS    WGS; ENV; Metagenome Assembled Genome; MAG; Third Party Data; TPA;
            TPA:assembly.
SOURCE      Micropruina sp. (wastewater metagenome)
  ORGANISM  Micropruina sp.
            Bacteria; Bacillati; Actinomycetota; Actinomycetes;
            Propionibacteriales; Nocardioidaceae; Micropruina.
REFERENCE   1  (bases 1 to 2058)
  AUTHORS   Abdulkadir,N., Saraiva,J.P., Zhang,J., Stolte,S., Gillor,O.,
            Harms,H. and Rocha,U.
  TITLE     Genome-centric analyses of 165 metagenomes show that mobile genetic
            elements are crucial for the transmission of antimicrobial
            resistance genes to pathogens in activated sludge and wastewater
  JOURNAL   Microbiol Spectr 12 (3), e0291823 (2024)
   PUBMED   38289113
REFERENCE   2  (bases 1 to 2058)
  AUTHORS   Abdulkadir,N.
  TITLE     Direct Submission
  JOURNAL   Submitted (24-MAR-2023) Environmental Microbiology, Helmholtz
            Center for Environmental Research -UFZ, Permoserstr 15, Leipzig
            04318, Germany
COMMENT     The annotation was added by the NCBI Prokaryotic Genome Annotation
            Pipeline (PGAP). Information about PGAP can be found here:
            https://www.ncbi.nlm.nih.gov/genome/annotation_prok/
            
            ##Genome-Assembly-Data-START##
            Assembly Method        :: SPAdes v. 3.11.1
            Genome Representation  :: Full
            Expected Final Version :: Yes
            Genome Coverage        :: Not Applicable
            Sequencing Technology  :: Illumina MiSeq
            ##Genome-Assembly-Data-END##
            
            ##Genome-Annotation-Data-START##
            Annotation Provider               :: NCBI
            Annotation Date                   :: 05/04/2023 04:57:10
            Annotation Pipeline               :: NCBI Prokaryotic Genome
                                                 Annotation Pipeline (PGAP)
            Annotation Method                 :: Best-placed reference protein
                                                 set; GeneMarkS-2+
            Annotation Software revision      :: 6.5
            Features Annotated                :: Gene; CDS; rRNA; tRNA; ncRNA
            Genes (total)                     :: 2,776
            CDSs (total)                      :: 2,750
            Genes (coding)                    :: 2,714
            CDSs (with protein)               :: 2,714
            Genes (RNA)                       :: 26
            tRNAs                             :: 24
            ncRNAs                            :: 2
            Pseudo Genes (total)              :: 36
            CDSs (without protein)            :: 36
            Pseudo Genes (ambiguous residues) :: 0 of 36
            Pseudo Genes (frameshifted)       :: 2 of 36
            Pseudo Genes (incomplete)         :: 32 of 36
            Pseudo Genes (internal stop)      :: 2 of 36
            ##Genome-Annotation-Data-END##
FEATURES             Location/Qualifiers
     source          1..2058
                     /organism="Micropruina sp."
                     /mol_type="genomic DNA"
                     /submitter_seqid="NODE_22069_length_2058_cov_5.232152"
                     /isolate="AMR_MDS_2195"
                     /isolation_source="Activated sludge of municipal
                     wastewater treatment plants"
                     /db_xref="taxon:2737536"
                     /environmental_sample
                     /geo_loc_name="Canada"
                     /lat_lon="49.25 N 123.1 W"
                     /collection_date="2015-06-16"
                     /metagenome_source="wastewater metagenome"
                     /note="metagenomic"
     gene            <1..707
                     /locus_tag="PLF56_13515"
     CDS             <1..707
                     /locus_tag="PLF56_13515"
                     /inference="COORDINATES: protein motif:HMM:NF012301.2"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_function: GO:0003677 - DNA binding [Evidence IEA];
                     GO_process: GO:0000160 - phosphorelay signal transduction
                     system [Evidence IEA];
                     GO_process: GO:0006355 - regulation of DNA-templated
                     transcription [Evidence IEA]"
                     /codon_start=3
                     /transl_table=11
                     /product="response regulator transcription factor"
                     /protein_id="HOQ54617.1"
                     /translation="SPTTPIVYDPDYEADSQRDENMAVPIRVVVVDDQPAMLTSLTRM
                     LGRVPVLECVGEGHDGFDAIRLVETLDPDVMLIDLLMPKMNGIEAIRRIAAMGPRPRL
                     IALTTLSDDQTFADALQAGAAGFLLKASTVGEIVHAVQVVHAGEAMLSPQLVTRLVAR
                     YTQQAAVDISGLGASDVALLRLVGQGLSNEEIAQQRHLSPATVKSYLSRLLAKTGCRD
                     RAQLVILAYRSGVAEL"
     gene            complement(789..>2058)
                     /locus_tag="PLF56_13520"
     CDS             complement(789..>2058)
                     /locus_tag="PLF56_13520"
                     /EC_number="1.1.1.261"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_014846860.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_function: GO:0030554 - adenyl nucleotide binding
                     [Evidence IEA];
                     GO_function: GO:0046872 - metal ion binding [Evidence
                     IEA]"
                     /codon_start=2
                     /transl_table=11
                     /product="sn-glycerol-1-phosphate dehydrogenase"
                     /protein_id="HOQ54618.1"
                     /translation="VADAYRATFGDRAAVVIGDERTMAVAGDAVLAGLKAGGVELVEP
                     YVFPGDPELYAKYENIETLRDALRDVDAVPVAVGSGTLNDIVKRAAGELGREYLVVCT
                     AASMDGYTAFGSSIAVDGFKQTLTCPAPAVCVADWTVMAAAPQVMTASGYGDLLGKVP
                     AGADWIIADALGAEAIDDYVWGLVQGPLRASLSKPESLASGDVPATGELAEGLVMSGL
                     AMQAHQSSRPASGAEHQFSHLWEMEGHGVDIKPRRLSHGFKVAVGSVAIAALYERLFE
                     RDLTGLDVDAAVAAYPDAAAMEARSRAAHQPHLVEEVVVQTMAKHLTPEQLRERLGLV
                     KSLWPELKPKLQAQLIPAAELQRMLRAVGAPAHPYDIALTWAEFKATYQRAQMIRKRY
                     TVLDLLLELNLLDELVAELFAPGGFWAEQE"
BASE COUNT          329 a          720 c          696 g          313 t
ORIGIN      
        1 gatcaccgac gaccccgata gtctacgacc ctgactacga agcggacagt cagcgggatg
       61 agaacatggc agtgccgatc agggtcgtcg tcgtcgacga tcagcccgcc atgctcacct
      121 ccctcacccg catgctgggc cgcgtcccgg tgctggaatg tgtgggcgag gggcacgacg
      181 gcttcgacgc catccggctg gtcgaaaccc tcgatccgga tgtgatgctg atcgatctgc
      241 tgatgcccaa gatgaacggc atcgaagcga tccggcggat cgcggcgatg ggtccccgac
      301 cccggctgat cgcactcacc accctgagtg atgaccagac cttcgccgac gccctacagg
      361 cgggtgccgc cggctttctg ctcaaggcca gcacggtcgg cgagatcgtg cacgcggtgc
      421 aggtggtgca cgccggcgag gcgatgctgt cgccgcagct cgtgacccgg ctggtggcgc
      481 gctacacgca gcaggcggcc gtcgacatca gcgggctggg tgcgagcgat gtcgcgttgc
      541 tgcgcctggt cggccagggg ctcagcaatg aggagatcgc gcagcaacgg cacctcagcc
      601 cggccacggt gaagagctac ctcagccggc tgttggcgaa gaccggctgc cgggaccgcg
      661 cccaactggt cattctcgcg tatcgcagcg gagtcgccga gctttagcct gctttagcct
      721 ggatccaggt gtaccccggg cacctcacgg ccgaggaccg gcaggctgga ggcctgccgg
      781 cgtggacgtc actcctgttc ggcccagaac cctccggggg cgaacaattc tgcgaccagc
      841 tcgtccagca gattgagttc gagcagcagg tcgagcaccg tgtagcgctt gcggatcatc
      901 tgggcgcgct ggtacgtcgc cttgaactcg gcccaggtca gggcgatgtc gtaggggtgc
      961 gcaggtgctc cgacggcccg cagcatgcgc tgcaactcgg cggccgggat cagttgcgcc
     1021 tgcagcttgg gcttgagctc gggccacagg gacttcacca gcccgagccg ctcgcggagc
     1081 tgctcgggtg tcaggtgctt cgccatggtc tgcacgacca cctcttcgac caggtgcggc
     1141 tggtgggcgg cccgcgaccg ggcctccatc gcggcagcgt cggggtaggc ggccactgcg
     1201 gcgtccacgt cgaggccggt caggtcgcgc tcgaacaacc gctcgtagag ggcggcgatc
     1261 gcgacactgc ccacggccac cttgaagccg tgcgacagcc gccggggttt gatatcgacg
     1321 ccgtggccct ccatctccca cagatggctg aactggtgct cggcaccgga ggccggccgc
     1381 gaggactggt gcgcctgcat cgccaggccc gacatgacca gcccctcggc caactcgccg
     1441 gtggccggca cgtcaccgga cgcgagtgac tcgggcttgc tcaacgacgc ccgcagcggg
     1501 ccctgcacca gcccccacac gtagtcgtcg atggcctcgg cgccgagcgc gtcggcgatg
     1561 atccagtcgg caccggccgg caccttgccg agcaggtcgc cgtagccgga cgcggtcatc
     1621 acctggggcg cggctgccat caccgtccag tcggccacac agaccgccgg ggcggggcag
     1681 gtcagggtct gtttgaaccc gtccacggcg atggacgagc cgaaggccgt gtagccgtcc
     1741 atggacgcgg cggtgcacac cacgaggtac tcacgcccga gttcaccggc cgcccgcttg
     1801 acgatgtcgt tcagggtgcc ggaaccgacc gccaccggaa ccgcgtccac gtcgcgcaac
     1861 gcgtcgcgca acgtttcgat gttctcgtac ttcgcgtaca actcgggatc gcccggaaac
     1921 acgtacggtt cgaccaactc gacgccgccc gccttcagcc cggccaggac ggcgtcaccg
     1981 gccaccgcca tggtgcgctc gtcgccgatc accacagcgg cacggtcgcc gaaggtggcc
     2041 cggtaggcgt ccgccacc
//
LOCUS       DAOSUG010000518         2047 bp    DNA     linear   ENV 10-JUN-2024
DEFINITION  MAG TPA_asm: Micropruina sp. isolate AMR_MDS_2195
            NODE_22223_length_2047_cov_4.023594, whole genome shotgun sequence.
ACCESSION   DAOSUG010000518 DAOSUG010000000
VERSION     DAOSUG010000518.1
DBLINK      BioProject: PRJNA925477
            BioSample: SAMN32864262
            Sequence Read Archive: SRR4236673
KEYWORDS    WGS; ENV; Metagenome Assembled Genome; MAG; Third Party Data; TPA;
            TPA:assembly.
SOURCE      Micropruina sp. (wastewater metagenome)
  ORGANISM  Micropruina sp.
            Bacteria; Bacillati; Actinomycetota; Actinomycetes;
            Propionibacteriales; Nocardioidaceae; Micropruina.
REFERENCE   1  (bases 1 to 2047)
  AUTHORS   Abdulkadir,N., Saraiva,J.P., Zhang,J., Stolte,S., Gillor,O.,
            Harms,H. and Rocha,U.
  TITLE     Genome-centric analyses of 165 metagenomes show that mobile genetic
            elements are crucial for the transmission of antimicrobial
            resistance genes to pathogens in activated sludge and wastewater
  JOURNAL   Microbiol Spectr 12 (3), e0291823 (2024)
   PUBMED   38289113
REFERENCE   2  (bases 1 to 2047)
  AUTHORS   Abdulkadir,N.
  TITLE     Direct Submission
  JOURNAL   Submitted (24-MAR-2023) Environmental Microbiology, Helmholtz
            Center for Environmental Research -UFZ, Permoserstr 15, Leipzig
            04318, Germany
COMMENT     The annotation was added by the NCBI Prokaryotic Genome Annotation
            Pipeline (PGAP). Information about PGAP can be found here:
            https://www.ncbi.nlm.nih.gov/genome/annotation_prok/
            
            ##Genome-Assembly-Data-START##
            Assembly Method        :: SPAdes v. 3.11.1
            Genome Representation  :: Full
            Expected Final Version :: Yes
            Genome Coverage        :: Not Applicable
            Sequencing Technology  :: Illumina MiSeq
            ##Genome-Assembly-Data-END##
            
            ##Genome-Annotation-Data-START##
            Annotation Provider               :: NCBI
            Annotation Date                   :: 05/04/2023 04:57:10
            Annotation Pipeline               :: NCBI Prokaryotic Genome
                                                 Annotation Pipeline (PGAP)
            Annotation Method                 :: Best-placed reference protein
                                                 set; GeneMarkS-2+
            Annotation Software revision      :: 6.5
            Features Annotated                :: Gene; CDS; rRNA; tRNA; ncRNA
            Genes (total)                     :: 2,776
            CDSs (total)                      :: 2,750
            Genes (coding)                    :: 2,714
            CDSs (with protein)               :: 2,714
            Genes (RNA)                       :: 26
            tRNAs                             :: 24
            ncRNAs                            :: 2
            Pseudo Genes (total)              :: 36
            CDSs (without protein)            :: 36
            Pseudo Genes (ambiguous residues) :: 0 of 36
            Pseudo Genes (frameshifted)       :: 2 of 36
            Pseudo Genes (incomplete)         :: 32 of 36
            Pseudo Genes (internal stop)      :: 2 of 36
            ##Genome-Annotation-Data-END##
FEATURES             Location/Qualifiers
     source          1..2047
                     /organism="Micropruina sp."
                     /mol_type="genomic DNA"
                     /submitter_seqid="NODE_22223_length_2047_cov_4.023594"
                     /isolate="AMR_MDS_2195"
                     /isolation_source="Activated sludge of municipal
                     wastewater treatment plants"
                     /db_xref="taxon:2737536"
                     /environmental_sample
                     /geo_loc_name="Canada"
                     /lat_lon="49.25 N 123.1 W"
                     /collection_date="2015-06-16"
                     /metagenome_source="wastewater metagenome"
                     /note="metagenomic"
     gene            <1..907
                     /gene="dapC"
                     /locus_tag="PLF56_13525"
     CDS             <1..907
                     /gene="dapC"
                     /locus_tag="PLF56_13525"
                     /EC_number="2.6.1.17"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_016669589.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_function: GO:0003824 - catalytic activity [Evidence
                     IEA];
                     GO_function: GO:0030170 - pyridoxal phosphate binding
                     [Evidence IEA];
                     GO_process: GO:0009058 - biosynthetic process [Evidence
                     IEA]"
                     /codon_start=2
                     /transl_table=11
                     /product="succinyldiaminopimelate transaminase"
                     /protein_id="HOQ54619.1"
                     /translation="LNYATSRLRTVLAVRWGAVPIADEAVLPVIGTKELVAQLPHLLG
                     LGTGDVVVIPEVAYPTYEVGARLAGAEVVACDDPSAIEGMRPALVWINSPANPHGTIL
                     SPADLGRWVVYCRERDAVLASDECYGEFGWQDEPVSVLHPQVNGDSLSGLLAVHSLSK
                     RSNAAGYRAGFVAGDPDLVTALRELRKHAGGMVPTPVQAAMAALLGDQQHVREQRERY
                     LRRRERLRPALQAAGFRIEHSEGSLYLWCTRDEPGRASVDWLAERGILVAPGDFYGAA
                     SSNHVRVALTATDERIEAAVARLMG"
     gene            913..1482
                     /locus_tag="PLF56_13530"
     CDS             913..1482
                     /locus_tag="PLF56_13530"
                     /inference="COORDINATES: protein motif:HMM:NF013989.2"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_function: GO:0008703 -
                     5-amino-6-(5-phosphoribosylamino)uracil reductase activity
                     [Evidence IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="dihydrofolate reductase family protein"
                     /protein_id="HOQ54620.1"
                     /translation="MGRLVYTSIGSLDGFIADADGAFDWSMPSEQVHAFLNARDRAVT
                     LELYGRRLYQVMKVWQSFGTEPDASAVERDYGEQWRGRDKVVCSSTLDAIDTERTRLV
                     RRFDPEQVRQLVHDAEGDVTIGGPTLAAHALRAGLVERIEYYLNPVVAGGGLAWLPPD
                     LRLGLRLLEQQRFDNGVIFVSYEVERASA"
BASE COUNT          288 a          654 c          766 g          339 t
ORIGIN      
        1 cctgaactac gccacgtcaa gattgaggac ggtcctggcc gtccgctggg gcgccgtccc
       61 gatcgcggac gaggctgtgc tgccggtgat cggcaccaaa gaactggtcg cccagttgcc
      121 gcacctgctc ggcctgggca ccggcgacgt ggtggtgatt cccgaggtgg cctacccgac
      181 ctacgaggtg ggagcgcggc tggccggtgc cgaggtggtg gcctgcgacg acccgtccgc
      241 gatcgaaggg atgcgtccgg cgctggtctg gatcaactca ccggccaatc cccacggcac
      301 gatcctgtcg cccgccgacc tgggccgctg ggtggtttat tgccgcgagc gcgacgccgt
      361 gctggcctcg gacgagtgct acggcgagtt cggctggcag gacgagcccg tctcggtgct
      421 gcacccgcag gtcaatggtg attcgttgag cgggttgctg gccgtgcatt cgttgtcgaa
      481 acggtccaat gcggccggct atcgggccgg cttcgttgcg ggcgaccccg acctggtcac
      541 ggccctgcgc gagttgcgca agcacgccgg cggcatggtg cccacccccg tccaggcggc
      601 gatggcggcg ctgctgggcg atcagcaaca cgtgcgtgag caacgcgaac ggtatctgcg
      661 acgtcgtgag cggctgcgtc cggcgctgca ggcggccggc tttcgcatcg agcactccga
      721 gggctcgctg tacctgtggt gcacccgcga cgagccgggc cgggccagcg tcgactggct
      781 ggccgagcgg ggcatcctgg tggctccggg cgacttctac ggcgcggcgt ccagcaacca
      841 cgtgcgggtg gcgctgaccg cgaccgacga gcgtatcgag gccgccgtgg ctcgactgat
      901 gggctgagct tcatgggccg gctggtgtac acctcgatcg ggtcgctcga cggcttcatc
      961 gccgacgccg acggcgcgtt cgactggagc atgcccagcg agcaggtgca cgcctttctc
     1021 aacgcccgcg accgggcggt gacgctggag ttgtacggac gccggctcta tcaggtgatg
     1081 aaggtctggc agagcttcgg caccgagccg gacgcgtccg cagtggaacg tgactacggc
     1141 gaacagtggc ggggccgcga caaggtggtg tgttcgtcga cgctcgatgc gatcgacacc
     1201 gagcgcaccc ggttggtgcg ccgcttcgat ccggagcagg tgcggcaact cgtgcacgac
     1261 gccgagggcg acgtcaccat cggcgggccg accctcgccg cgcatgcgct gcgggccggc
     1321 ttggtcgagc gaatcgagta ctacctgaac ccggtcgtgg cgggtggtgg gctggcctgg
     1381 ctgccgccgg atctcagact cggtctgcgg ctgctcgagc agcagaggtt cgacaacggc
     1441 gtgatcttcg tgtcgtacga ggtggagcgg gccagtgcgt aggccgcccc aaccccgttg
     1501 cccgattcgc gagggcgagc cgtgcacgct ctgctttcct ggtgcggacg gcccgcacaa
     1561 ctgcggtctg gtgtacctgg tgcagtccga ccccgacctt cgagaggaac gcgcccagcg
     1621 ccggacgcag cggggcgcga ccgccggctg agtgcggctg ggacgccggt tggcgtctcg
     1681 gcaagctaac cagcggtgcg gatgctggtt gggtttggac aggctcgatg aacggtacgg
     1741 atgtagcggg gctcgaacgc cggttgttca ggcggggatg caggcctcag gtacgggacg
     1801 ttgtcgttga gggtgatcgc gcgcataatg tcgaaacctg cgcgccagtg accgacgcca
     1861 cggcgtgtcg ctggtccgga gcgccactgg ggcgcggatc tcgtaagtgt tgtgcgagga
     1921 cgtgtgggct cgggccgctc agccgcggaa cgtgacggtg acgacccgtc cggagcccgt
     1981 gcccgtccag tcggcgaggt tgcgggtgtc gagcgtccag ttggcaccgc cgaggctcac
     2041 cctcgcc
//
LOCUS       DAOSUG010000519         2041 bp    DNA     linear   ENV 10-JUN-2024
DEFINITION  MAG TPA_asm: Micropruina sp. isolate AMR_MDS_2195
            NODE_22325_length_2041_cov_3.232628, whole genome shotgun sequence.
ACCESSION   DAOSUG010000519 DAOSUG010000000
VERSION     DAOSUG010000519.1
DBLINK      BioProject: PRJNA925477
            BioSample: SAMN32864262
            Sequence Read Archive: SRR4236673
KEYWORDS    WGS; ENV; Metagenome Assembled Genome; MAG; Third Party Data; TPA;
            TPA:assembly.
SOURCE      Micropruina sp. (wastewater metagenome)
  ORGANISM  Micropruina sp.
            Bacteria; Bacillati; Actinomycetota; Actinomycetes;
            Propionibacteriales; Nocardioidaceae; Micropruina.
REFERENCE   1  (bases 1 to 2041)
  AUTHORS   Abdulkadir,N., Saraiva,J.P., Zhang,J., Stolte,S., Gillor,O.,
            Harms,H. and Rocha,U.
  TITLE     Genome-centric analyses of 165 metagenomes show that mobile genetic
            elements are crucial for the transmission of antimicrobial
            resistance genes to pathogens in activated sludge and wastewater
  JOURNAL   Microbiol Spectr 12 (3), e0291823 (2024)
   PUBMED   38289113
REFERENCE   2  (bases 1 to 2041)
  AUTHORS   Abdulkadir,N.
  TITLE     Direct Submission
  JOURNAL   Submitted (24-MAR-2023) Environmental Microbiology, Helmholtz
            Center for Environmental Research -UFZ, Permoserstr 15, Leipzig
            04318, Germany
COMMENT     The annotation was added by the NCBI Prokaryotic Genome Annotation
            Pipeline (PGAP). Information about PGAP can be found here:
            https://www.ncbi.nlm.nih.gov/genome/annotation_prok/
            
            ##Genome-Assembly-Data-START##
            Assembly Method        :: SPAdes v. 3.11.1
            Genome Representation  :: Full
            Expected Final Version :: Yes
            Genome Coverage        :: Not Applicable
            Sequencing Technology  :: Illumina MiSeq
            ##Genome-Assembly-Data-END##
            
            ##Genome-Annotation-Data-START##
            Annotation Provider               :: NCBI
            Annotation Date                   :: 05/04/2023 04:57:10
            Annotation Pipeline               :: NCBI Prokaryotic Genome
                                                 Annotation Pipeline (PGAP)
            Annotation Method                 :: Best-placed reference protein
                                                 set; GeneMarkS-2+
            Annotation Software revision      :: 6.5
            Features Annotated                :: Gene; CDS; rRNA; tRNA; ncRNA
            Genes (total)                     :: 2,776
            CDSs (total)                      :: 2,750
            Genes (coding)                    :: 2,714
            CDSs (with protein)               :: 2,714
            Genes (RNA)                       :: 26
            tRNAs                             :: 24
            ncRNAs                            :: 2
            Pseudo Genes (total)              :: 36
            CDSs (without protein)            :: 36
            Pseudo Genes (ambiguous residues) :: 0 of 36
            Pseudo Genes (frameshifted)       :: 2 of 36
            Pseudo Genes (incomplete)         :: 32 of 36
            Pseudo Genes (internal stop)      :: 2 of 36
            ##Genome-Annotation-Data-END##
FEATURES             Location/Qualifiers
     source          1..2041
                     /organism="Micropruina sp."
                     /mol_type="genomic DNA"
                     /submitter_seqid="NODE_22325_length_2041_cov_3.232628"
                     /isolate="AMR_MDS_2195"
                     /isolation_source="Activated sludge of municipal
                     wastewater treatment plants"
                     /db_xref="taxon:2737536"
                     /environmental_sample
                     /geo_loc_name="Canada"
                     /lat_lon="49.25 N 123.1 W"
                     /collection_date="2015-06-16"
                     /metagenome_source="wastewater metagenome"
                     /note="metagenomic"
     gene            257..1489
                     /gene="istA"
                     /locus_tag="PLF56_13535"
     CDS             257..1489
                     /gene="istA"
                     /locus_tag="PLF56_13535"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_020391749.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_function: GO:0004803 - transposase activity [Evidence
                     IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="IS21 family transposase"
                     /protein_id="HOQ54621.1"
                     /translation="MISVDVWAEIRYLHASEGLSGRAIAKRLGVARKTVAKALASDGP
                     PVYKARPPVESAWARVEPAVRVLLSEFPDMPATVLAQRVGWDGSITWFRENVAETRRR
                     VRRVDPADRLVHRPGEQVQCDLWFPPVDVGLGPRLRGRVPVLVMVAAHSRRIEAVMVP
                     SRRTGDLLAGMWLLLQRFGGVPKRLLWDNEAGIGRRGRLADGVSGFCGTLGTKLVQAK
                     PYDPETKGIVERSNQYLETSFLPGRRFTNPQDFNTQLKGWLDEVGNQRQVRALGARPA
                     EVFAADLAAMTALPPSPPTVGERFTIRLGRDYYVRAAGNDYSVDPAMIERLVTVNVSL
                     DEVTVTYQGLPVARHDRAWGSGHTLTDPAHVETAARLRRDFQHPPVTVLEEGLVRDLA
                     VYDQAFGLAAADFDGQVA"
     gene            1489..>2041
                     /locus_tag="PLF56_13540"
     CDS             1489..>2041
                     /locus_tag="PLF56_13540"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_020391750.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_function: GO:0005524 - ATP binding [Evidence IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="ATP-binding protein"
                     /protein_id="HOQ54622.1"
                     /translation="MPTAADETFTRIDYLARVMKAPRIRESARRLAGQAAAQAWTGPQ
                     YLQAVLEAEVAAREASGAKLRLRAAGFPAVKTLEEFNFDHQPAADRAQLHRLAAGGYL
                     TEAGNVVLLGPPGTGKTHLATALAVAACHQQIRVGFDTAAGWVTRLQTAHRTGRLDAE
                     LRKLARLQLLVIDEVGYLPFDADA"
BASE COUNT          313 a          699 c          703 g          326 t
ORIGIN      
        1 ggcgtctcct ggcagggaca cctcggtggc gcgatcggcg gcgtcatcgc cgcgtggttg
       61 ctgcacgccg accgctccca ggcccgccgc cccgccagcc tcaggactgg ccgctgacac
      121 ccaaccggac cgagtgaccg gcccaccagc gcacgaagtc tcggcagctc gctcatgcga
      181 ccagcctgtc gactacggtc gaaaagggag ccagtaacga cggttgaaaa gggggccatg
      241 cgatcacgat ggagggatga tcagcgtgga tgtgtgggcc gagattcggt atctgcatgc
      301 tagtgagggc ttgtcgggta gggcgatcgc gaagcggttg ggggtggctc gcaagacggt
      361 cgcgaaggcg ttggcctcgg atggtccgcc tgtttacaag gctcgcccgc cggtcgagtc
      421 agcctgggcc cgggtcgaac cggcagtgcg agtgttgctg agtgagttcc cggacatgcc
      481 ggcgacggtg ttggcgcagc gggtcggttg ggacgggtcg atcacctggt tccgtgagaa
      541 cgtcgcggag acacgtcggc gggtccgcag ggttgatccg gcggatcggc tcgtccaccg
      601 cccgggggag caggtccagt gcgacttgtg gttcccgccc gtggacgttg gtctcgggcc
      661 caggttgcgg ggtcgggtgc cggtgttggt gatggtcgcg gcgcattccc gccggatcga
      721 ggcggtgatg gtccccagcc gtcgtaccgg tgatctgttg gcggggatgt ggttgttgtt
      781 gcagcgtttc ggtggcgtcc cgaaacggct gctgtgggac aacgaggccg gtatcggacg
      841 ccgtggacgc ctcgctgacg gggtcagcgg cttctgcggc accttgggca cgaaactcgt
      901 gcaggccaag ccgtatgacc cggagaccaa aggcatcgtg gagcggtcca accagtacct
      961 ggagacctcc ttcctgcccg gccgccgctt caccaacccg caggacttca acacccagct
     1021 gaagggttgg ctcgacgagg tcggcaacca gcgccaggtg agggccctgg gggcccgccc
     1081 cgcggaggtg ttcgccgcgg acctggctgc catgacggcc ctgccgccca gcccgccgac
     1141 ggtgggtgaa cggttcacga tccgactggg ccgcgactac tacgtccgcg ccgccggcaa
     1201 cgactactcc gttgacccgg ccatgatcga gcgtctcgtc accgtgaacg tgagcttgga
     1261 cgaggtgacg gtgacctacc aaggcctccc ggtcgcccgg catgaccggg cctggggttc
     1321 gggccacacc ctcaccgacc cggcccatgt cgagaccgcc gcccgactgc gccgagactt
     1381 ccaacacccg cccgtcacgg tcctcgagga gggcctggtt cgggaccttg cggtctatga
     1441 ccaggccttc ggcctcgcag cagctgattt cgacgggcag gttgcctgat gcccaccgcc
     1501 gctgacgaaa cgttcacccg catcgactac ctcgcccggg tgatgaaagc accccgcatc
     1561 cgcgagtcgg cccggcggtt ggctggccag gccgctgccc aagcctggac cggcccccag
     1621 tacttgcagg ccgtcctcga ggccgaggtc gctgcccgag aggcctccgg cgcgaaacta
     1681 cgcctgcgcg cggcaggatt ccccgcggtc aagacgttgg aggagttcaa cttcgaccac
     1741 caacccgccg ctgacagggc ccagctccac cgcctggcgg ccggcggcta cctgaccgag
     1801 gccggcaatg tcgtgctgct cggaccaccc ggcaccggca agactcacct cgccaccgcc
     1861 ctggccgtcg ccgcctgcca ccaacagatt cgtgtcgggt tcgacaccgc cgccggttgg
     1921 gtcacccgac tgcagaccgc tcaccgcacc ggccgactcg atgcggagct acgcaaactg
     1981 gcccggctgc agctgctggt catcgacgag gtcggctacc tgcccttcga cgccgacgcc
     2041 g
//
LOCUS       DAOSUG010000520         2035 bp    DNA     linear   ENV 10-JUN-2024
DEFINITION  MAG TPA_asm: Micropruina sp. isolate AMR_MDS_2195
            NODE_22408_length_2035_cov_5.813636, whole genome shotgun sequence.
ACCESSION   DAOSUG010000520 DAOSUG010000000
VERSION     DAOSUG010000520.1
DBLINK      BioProject: PRJNA925477
            BioSample: SAMN32864262
            Sequence Read Archive: SRR4236673
KEYWORDS    WGS; ENV; Metagenome Assembled Genome; MAG; Third Party Data; TPA;
            TPA:assembly.
SOURCE      Micropruina sp. (wastewater metagenome)
  ORGANISM  Micropruina sp.
            Bacteria; Bacillati; Actinomycetota; Actinomycetes;
            Propionibacteriales; Nocardioidaceae; Micropruina.
REFERENCE   1  (bases 1 to 2035)
  AUTHORS   Abdulkadir,N., Saraiva,J.P., Zhang,J., Stolte,S., Gillor,O.,
            Harms,H. and Rocha,U.
  TITLE     Genome-centric analyses of 165 metagenomes show that mobile genetic
            elements are crucial for the transmission of antimicrobial
            resistance genes to pathogens in activated sludge and wastewater
  JOURNAL   Microbiol Spectr 12 (3), e0291823 (2024)
   PUBMED   38289113
REFERENCE   2  (bases 1 to 2035)
  AUTHORS   Abdulkadir,N.
  TITLE     Direct Submission
  JOURNAL   Submitted (24-MAR-2023) Environmental Microbiology, Helmholtz
            Center for Environmental Research -UFZ, Permoserstr 15, Leipzig
            04318, Germany
COMMENT     The annotation was added by the NCBI Prokaryotic Genome Annotation
            Pipeline (PGAP). Information about PGAP can be found here:
            https://www.ncbi.nlm.nih.gov/genome/annotation_prok/
            
            ##Genome-Assembly-Data-START##
            Assembly Method        :: SPAdes v. 3.11.1
            Genome Representation  :: Full
            Expected Final Version :: Yes
            Genome Coverage        :: Not Applicable
            Sequencing Technology  :: Illumina MiSeq
            ##Genome-Assembly-Data-END##
            
            ##Genome-Annotation-Data-START##
            Annotation Provider               :: NCBI
            Annotation Date                   :: 05/04/2023 04:57:10
            Annotation Pipeline               :: NCBI Prokaryotic Genome
                                                 Annotation Pipeline (PGAP)
            Annotation Method                 :: Best-placed reference protein
                                                 set; GeneMarkS-2+
            Annotation Software revision      :: 6.5
            Features Annotated                :: Gene; CDS; rRNA; tRNA; ncRNA
            Genes (total)                     :: 2,776
            CDSs (total)                      :: 2,750
            Genes (coding)                    :: 2,714
            CDSs (with protein)               :: 2,714
            Genes (RNA)                       :: 26
            tRNAs                             :: 24
            ncRNAs                            :: 2
            Pseudo Genes (total)              :: 36
            CDSs (without protein)            :: 36
            Pseudo Genes (ambiguous residues) :: 0 of 36
            Pseudo Genes (frameshifted)       :: 2 of 36
            Pseudo Genes (incomplete)         :: 32 of 36
            Pseudo Genes (internal stop)      :: 2 of 36
            ##Genome-Annotation-Data-END##
FEATURES             Location/Qualifiers
     source          1..2035
                     /organism="Micropruina sp."
                     /mol_type="genomic DNA"
                     /submitter_seqid="NODE_22408_length_2035_cov_5.813636"
                     /isolate="AMR_MDS_2195"
                     /isolation_source="Activated sludge of municipal
                     wastewater treatment plants"
                     /db_xref="taxon:2737536"
                     /environmental_sample
                     /geo_loc_name="Canada"
                     /lat_lon="49.25 N 123.1 W"
                     /collection_date="2015-06-16"
                     /metagenome_source="wastewater metagenome"
                     /note="metagenomic"
     gene            complement(<1..950)
                     /locus_tag="PLF56_13545"
     CDS             complement(<1..950)
                     /locus_tag="PLF56_13545"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_002531705.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_function: GO:0046912 - acyltransferase activity, acyl
                     groups converted into alkyl on transfer [Evidence IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="citrate/2-methylcitrate synthase"
                     /protein_id="HOQ54623.1"
                     /translation="MSAAQFVRDDERCELPEVRASVGSNGYDLGKLLATTGSVTLDPG
                     FGNTASCTSAITYIDGDAGILRYRGYPIEQLAERSTFLETSYLVLYGELPTAEQLADF
                     TDQISTHHLLDERLKAMFGLFPRRSHPMPVLAAGINALSTFEMAPVALTEKSIEQHAY
                     RLMAKVPTLAAYGYKNSLGLPTLYPDESLGYIENFLRMSFGLPTRTYEIDPTIVRALD
                     VLLILHADHEQNCSTSTVRMVGSSGANLYLSVAAGVSALSGPLHGGANQAVIEMLQQI
                     RESGEDVATFVQKVKDKKSGVKLMGFGHRIYKNFDPRAQIV"
     gene            1214..1759
                     /locus_tag="PLF56_13550"
     CDS             1214..1759
                     /locus_tag="PLF56_13550"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_012922161.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_component: GO:0042597 - periplasmic space [Evidence
                     IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="YceI family protein"
                     /protein_id="HOQ54624.1"
                     /translation="MTTLTDLKGTYVLDPSHSQLGFVARHAMVTKVRGSFNEVTGTAT
                     IDGANPAASTLNVTVDTASVDTRNEGRDAHLKSPDFFDIEKFPEITFAGTDFAIDGDV
                     VNVTGDLTIKGNTHPVTIPLEFQGEAKDPFGNDRIGFEGSVVVNRKDWDLTWNAALET
                     GGVLVSEKVTLELEVSAIKQA"
BASE COUNT          353 a          695 c          665 g          322 t
ORIGIN      
        1 acgatctggg cgcgggggtc gaagttcttg tagatgcggt gcccgaagcc catcagcttg
       61 acgcccgact tcttgtcctt caccttctgg acgaacgtgg cgacgtcttc gcccgactcg
      121 cggatctgct gcaacatctc gatcacggcc tggttggcgc cgccgtgcag cgggcccgac
      181 agcgcgctca ccccggcggc caccgacagg tacaggttgg ccccggacga gccgaccatg
      241 cgcaccgtcg aggtggagca gttctgttcg tggtcggcgt gcaggatcag cagcacgtcc
      301 agcgcccgga cgatggtggg gtcgatctcg taggtgcggg tgggcagccc gaagctcatg
      361 cgcagaaagt tctcgatgta gcccagcgac tcatcggggt acagggtggg caggccgagc
      421 gagttcttgt agccgtaggc ggccagggtg ggcaccttgg ccatcaaccg gtaggcgtgc
      481 tgttcgatcg acttctcggt gagcgcgacc ggggccatct cgaaggtgga cagcgcgttg
      541 ataccggccg ccagcaccgg catcgggtgc gaacgccgcg gaaacaggcc aaacatcgcc
      601 ttcagccgct catccagcag gtggtgggtg ctgatctggt cggtgaagtc ggccaactgc
      661 tcggcggtgg gcaattcgcc gtacagcacc aggtaggacg tttcgagaaa cgtggaccgc
      721 tcggccaact gctcgatcgg gtagccgcga taccgcagaa tgccggcgtc gccgtcgatg
      781 taggtgatgg ccgacgtgca ggacgcggtg ttgccgaagc cggggtcgag ggtgaccgac
      841 ccggtggtgg ccagcagctt gccgaggtcg tagccgttgc tgccgacgct ggcccgcacc
      901 tcgggcagtt cgcaccgctc atcgtcccgg acgaactggg ctgcgctcat ggaagctcct
      961 ttcgggggcg acggggttcg gacgggggct acgtcgcctc accgccaccc tagtcggacg
     1021 cgccgacggt ggcggatcgt cgagcggaag cgatcgtcgg ggaaccggtc tcggccggcc
     1081 ggggacctcg gccgctgagt cgcccgccac acgattcccg atccgttcac gagcgtttgg
     1141 ttgacatgtc aaccaaacgc ctctacagtg aaggtagttg agatttcaag cacgaccccg
     1201 aggagcctgt tccatgacca cactgaccga tctcaagggc acgtacgtgc tcgaccccag
     1261 ccacagccag ctcggcttcg tcgcccgtca cgccatggtc accaaggtac gtggcagctt
     1321 caacgaggtc accggcaccg ccacgatcga cggcgccaac cccgccgcct cgacgctgaa
     1381 cgtcaccgtc gacacggcgt ccgtcgacac ccgcaacgag ggccgcgacg cgcacctgaa
     1441 gtcgcccgac ttcttcgaca tcgagaagtt ccccgagatc acctttgccg gcaccgactt
     1501 cgcgatcgac ggcgacgtgg tcaacgtcac cggcgacctg accatcaagg gcaacacgca
     1561 cccggtcacc attccgctcg agttccaggg cgaggccaag gaccccttcg gcaacgaccg
     1621 catcggcttc gaagggtcgg tcgtggtgaa ccgcaaggac tgggacctca cctggaacgc
     1681 cgctctcgag accggcggcg tgctggtcag cgagaaggtc accctcgaac tcgaggtctc
     1741 tgccatcaag caggcctgac ctcgtcccgc acagcggccc ggcccggggg gtgccgggcc
     1801 gctgtgctgt gcccgtccgg acgtcaactc gcccgtggga cgcatcgcgc acgccggcca
     1861 tcccgcggta cgacgtcgcg cgcgcaggcc atcccgcggt acaacatcgc gcacgccggc
     1921 catcccgcgg tacaacatcg cgcacgccgg ccatccggcg gtacaacatc gcgcactctt
     1981 gcgttcatcg cgcgcggacg aacccgcgca acgaaccctt gaccgcgcaa cgtcg
//
LOCUS       DAOSUG010000521         2029 bp    DNA     linear   ENV 10-JUN-2024
DEFINITION  MAG TPA_asm: Micropruina sp. isolate AMR_MDS_2195
            NODE_22497_length_2029_cov_3.953901, whole genome shotgun sequence.
ACCESSION   DAOSUG010000521 DAOSUG010000000
VERSION     DAOSUG010000521.1
DBLINK      BioProject: PRJNA925477
            BioSample: SAMN32864262
            Sequence Read Archive: SRR4236673
KEYWORDS    WGS; ENV; Metagenome Assembled Genome; MAG; Third Party Data; TPA;
            TPA:assembly.
SOURCE      Micropruina sp. (wastewater metagenome)
  ORGANISM  Micropruina sp.
            Bacteria; Bacillati; Actinomycetota; Actinomycetes;
            Propionibacteriales; Nocardioidaceae; Micropruina.
REFERENCE   1  (bases 1 to 2029)
  AUTHORS   Abdulkadir,N., Saraiva,J.P., Zhang,J., Stolte,S., Gillor,O.,
            Harms,H. and Rocha,U.
  TITLE     Genome-centric analyses of 165 metagenomes show that mobile genetic
            elements are crucial for the transmission of antimicrobial
            resistance genes to pathogens in activated sludge and wastewater
  JOURNAL   Microbiol Spectr 12 (3), e0291823 (2024)
   PUBMED   38289113
REFERENCE   2  (bases 1 to 2029)
  AUTHORS   Abdulkadir,N.
  TITLE     Direct Submission
  JOURNAL   Submitted (24-MAR-2023) Environmental Microbiology, Helmholtz
            Center for Environmental Research -UFZ, Permoserstr 15, Leipzig
            04318, Germany
COMMENT     The annotation was added by the NCBI Prokaryotic Genome Annotation
            Pipeline (PGAP). Information about PGAP can be found here:
            https://www.ncbi.nlm.nih.gov/genome/annotation_prok/
            
            ##Genome-Assembly-Data-START##
            Assembly Method        :: SPAdes v. 3.11.1
            Genome Representation  :: Full
            Expected Final Version :: Yes
            Genome Coverage        :: Not Applicable
            Sequencing Technology  :: Illumina MiSeq
            ##Genome-Assembly-Data-END##
            
            ##Genome-Annotation-Data-START##
            Annotation Provider               :: NCBI
            Annotation Date                   :: 05/04/2023 04:57:10
            Annotation Pipeline               :: NCBI Prokaryotic Genome
                                                 Annotation Pipeline (PGAP)
            Annotation Method                 :: Best-placed reference protein
                                                 set; GeneMarkS-2+
            Annotation Software revision      :: 6.5
            Features Annotated                :: Gene; CDS; rRNA; tRNA; ncRNA
            Genes (total)                     :: 2,776
            CDSs (total)                      :: 2,750
            Genes (coding)                    :: 2,714
            CDSs (with protein)               :: 2,714
            Genes (RNA)                       :: 26
            tRNAs                             :: 24
            ncRNAs                            :: 2
            Pseudo Genes (total)              :: 36
            CDSs (without protein)            :: 36
            Pseudo Genes (ambiguous residues) :: 0 of 36
            Pseudo Genes (frameshifted)       :: 2 of 36
            Pseudo Genes (incomplete)         :: 32 of 36
            Pseudo Genes (internal stop)      :: 2 of 36
            ##Genome-Annotation-Data-END##
FEATURES             Location/Qualifiers
     source          1..2029
                     /organism="Micropruina sp."
                     /mol_type="genomic DNA"
                     /submitter_seqid="NODE_22497_length_2029_cov_3.953901"
                     /isolate="AMR_MDS_2195"
                     /isolation_source="Activated sludge of municipal
                     wastewater treatment plants"
                     /db_xref="taxon:2737536"
                     /environmental_sample
                     /geo_loc_name="Canada"
                     /lat_lon="49.25 N 123.1 W"
                     /collection_date="2015-06-16"
                     /metagenome_source="wastewater metagenome"
                     /note="metagenomic"
     gene            complement(40..318)
                     /locus_tag="PLF56_13555"
     CDS             complement(40..318)
                     /locus_tag="PLF56_13555"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_011756565.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="Lrp/AsnC ligand binding domain-containing
                     protein"
                     /protein_id="HOQ54625.1"
                     /translation="MITAIVFVEAEVARIPEVAEQIANLEGVSEVYSVTGSIDLIALI
                     RVRSVEQVATVVTERLNKVVGVVNTETHIAFRAYSSHDLEAAFSLGAE"
     gene            complement(339..>2029)
                     /locus_tag="PLF56_13560"
     CDS             complement(339..>2029)
                     /locus_tag="PLF56_13560"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_020390388.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_function: GO:0003724 - RNA helicase activity [Evidence
                     IEA];
                     GO_function: GO:0005524 - ATP binding [Evidence IEA];
                     GO_process: GO:0000027 - ribosomal large subunit assembly
                     [Evidence IEA]"
                     /codon_start=3
                     /transl_table=11
                     /product="DEDD exonuclease domain-containing protein"
                     /protein_id="HOQ54626.1"
                     /translation="LATVTFCVVDLETTGGAADDRITEIGAVKVRGGEVLGEFQTLVN
                     PHTHIPPLIAVLTGITDGMVAQAPPLSSVLPGFMEFCRGCVLVAHNAGFDTGFLKRAC
                     AQLGYDWPNPAVVDTVGLARQALLRDEVRNHKLATLAEHFHADTTPNHRALTDARATV
                     TVLHGLLERVGNLGVATIEDLLEFTRRVSPGRRAKRTWAAGLPKAPGVYWFVADSRDG
                     AGRPRCQVLYVGKSRNLAARVRSYFTAAETRPRIDEMVRIATGIEHLVCATELQAEVL
                     ELRLIAAHSPRYNRRSKFPERQVWLKVTDEPFPRLSIVRQCLNDDASYFGPFSRRSSA
                     EAVQAALYDAFPIRQCTTRLSPKRPSASCVLGEMGRCVAPCELAITPEQYGALVDQVR
                     ASMTSDIRPVLTAARGRLATLIGRQRFEDAAVVAARLNGFASSALRHHRVASLARCAQ
                     IVAAQRHEGGWTIHVIRYGRLAGAARARPGDVPQAVARSAVASAEQVARPVGPQPAAT
                     TEETERIAAWLERPGVRLIEIEGEWSWPLHIGLAEGELARHALDGAPVPTPQAG"
BASE COUNT          322 a          676 c          735 g          296 t
ORIGIN      
        1 tgcgatcgcc gaaggggcga cgcatggaag agccgtccct cactcagcac ccagactgaa
       61 cgccgcctcg agatcgtgcg aactgtaggc gcgaaatgcg atgtgcgtct cggtgttcac
      121 cacgccgacg accttgttga gccgctcggt caccaccgtc gccacctgct cgacactgcg
      181 cacccggatc agcgcgatca ggtcgatgga gcccgtcacc gagtagacct cggatacccc
      241 ctcgaggttg gcgatctgtt cggctacctc gggaatgcgg gccacctcgg cctcgacgaa
      301 cacaatggcg gtgatcatgg cggcatagta gtgcgggatc agcccgcctg tggggtcggc
      361 acgggggcac cgtcgagcgc gtgccgggcc aactcaccct cggcgagacc gatgtgcaac
      421 ggccagctcc actcgccctc gatctcgatc agccggacgc cgggtcgctc gagccaggcg
      481 gcgatgcgtt cggtctcttc ggtggtggcc gccggctgtg gacccaccgg acgcgccacc
      541 tgctcggcgc tggccaccgc cgatcgggcc accgcctggg gcacgtcgcc cgggcgggcg
      601 cgggccgcac cggcgagccg tccgtaacgg atcacgtgaa tggtccagcc gccctcgtgg
      661 cgctgggcgg ccacgatctg ggcgcaacgg gccaggctgg ccacgcggtg atggcgcagt
      721 gcgctgctgg cgaaaccgtt gagccgagcc gcgaccacgg cggcgtcctc gaaccgctgc
      781 cgcccgatca gagtggccag ccggccccgg gccgcggtga gtaccggacg aatgtcgctg
      841 gtcatgctgg cgcgcacctg gtcgaccagc gccccgtact gctcaggagt gatcgccaac
      901 tcgcacgggg cgacgcagcg acccatttcg ccgagcacgc acgaggccga gggacgcttc
      961 ggggacagcc gggtggtgca ctgacggatc gggaacgcgt cgtagagggc ggcctgcact
     1021 gcctcggcgc tgctgcgccg gctgaacggg ccgaagtagg acgcgtcgtc gttcaggcac
     1081 tggcgcacga tggagagccg gggaaagggc tcatcggtca ccttgagcca gacctgccgc
     1141 tcgggaaact tggaccggcg gttgtagcga ggggaatggg cggcgatcag ccgcagttcg
     1201 agcacctcgg cctgcagttc ggtggcgcag accagatgct cgatgccggt cgcgatgcgc
     1261 accatctcgt cgatgcgtgg ccgggtttcg gcggcggtga agtagctgcg cacccgggcg
     1321 gccaggttgc ggctcttgcc gacgtacaac acctgacacc gcggacggcc agcgccgtca
     1381 cgggagtcgg cgacgaacca gtagacgccc ggcgccttgg gcagcccggc ggcccaggtt
     1441 cgcttggccc gccggccggg tgagacccgg cgggtgaact cgagcaggtc ttcaatggtg
     1501 gcgacgccga ggttaccgac ccgctcgagc aggccgtgca gcaccgtgac agtggcacgg
     1561 gcgtcggtga gcgcgcgatg gttcggggtg gtgtcggcgt ggaaatgctc ggccagggtg
     1621 gccagcttgt ggttgcgcac ttcgtcgcgc agcagcgctt gccgggccag gccgaccgtg
     1681 tcgaccacgg ccggattcgg ccagtcgtac ccgagttgcg cgcaggcacg cttcaagaag
     1741 ccggtgtcga agcccgcgtt gtgggccacc agcacgcagc cgcggcagaa ctccatgaac
     1801 ccgggcaaca ccgatgacag gggcggggcc tgggcgacca tgccgtcggt gatgccggtc
     1861 agcaccgcga tcaacggcgg aatgtgggtg tgtggattga ccagcgtctg aaactcgccg
     1921 agcacctcac cgccgcgcac cttgaccgca ccgatctcgg tgatgcggtc gtcggcggca
     1981 ccgccggtgg tttcgaggtc gaccacgcag aacgtgaccg tggcaaggg
//
LOCUS       DAOSUG010000522         2029 bp    DNA     linear   ENV 10-JUN-2024
DEFINITION  MAG TPA_asm: Micropruina sp. isolate AMR_MDS_2195
            NODE_22500_length_2029_cov_3.258865, whole genome shotgun sequence.
ACCESSION   DAOSUG010000522 DAOSUG010000000
VERSION     DAOSUG010000522.1
DBLINK      BioProject: PRJNA925477
            BioSample: SAMN32864262
            Sequence Read Archive: SRR4236673
KEYWORDS    WGS; ENV; Metagenome Assembled Genome; MAG; Third Party Data; TPA;
            TPA:assembly.
SOURCE      Micropruina sp. (wastewater metagenome)
  ORGANISM  Micropruina sp.
            Bacteria; Bacillati; Actinomycetota; Actinomycetes;
            Propionibacteriales; Nocardioidaceae; Micropruina.
REFERENCE   1  (bases 1 to 2029)
  AUTHORS   Abdulkadir,N., Saraiva,J.P., Zhang,J., Stolte,S., Gillor,O.,
            Harms,H. and Rocha,U.
  TITLE     Genome-centric analyses of 165 metagenomes show that mobile genetic
            elements are crucial for the transmission of antimicrobial
            resistance genes to pathogens in activated sludge and wastewater
  JOURNAL   Microbiol Spectr 12 (3), e0291823 (2024)
   PUBMED   38289113
REFERENCE   2  (bases 1 to 2029)
  AUTHORS   Abdulkadir,N.
  TITLE     Direct Submission
  JOURNAL   Submitted (24-MAR-2023) Environmental Microbiology, Helmholtz
            Center for Environmental Research -UFZ, Permoserstr 15, Leipzig
            04318, Germany
COMMENT     The annotation was added by the NCBI Prokaryotic Genome Annotation
            Pipeline (PGAP). Information about PGAP can be found here:
            https://www.ncbi.nlm.nih.gov/genome/annotation_prok/
            
            ##Genome-Assembly-Data-START##
            Assembly Method        :: SPAdes v. 3.11.1
            Genome Representation  :: Full
            Expected Final Version :: Yes
            Genome Coverage        :: Not Applicable
            Sequencing Technology  :: Illumina MiSeq
            ##Genome-Assembly-Data-END##
            
            ##Genome-Annotation-Data-START##
            Annotation Provider               :: NCBI
            Annotation Date                   :: 05/04/2023 04:57:10
            Annotation Pipeline               :: NCBI Prokaryotic Genome
                                                 Annotation Pipeline (PGAP)
            Annotation Method                 :: Best-placed reference protein
                                                 set; GeneMarkS-2+
            Annotation Software revision      :: 6.5
            Features Annotated                :: Gene; CDS; rRNA; tRNA; ncRNA
            Genes (total)                     :: 2,776
            CDSs (total)                      :: 2,750
            Genes (coding)                    :: 2,714
            CDSs (with protein)               :: 2,714
            Genes (RNA)                       :: 26
            tRNAs                             :: 24
            ncRNAs                            :: 2
            Pseudo Genes (total)              :: 36
            CDSs (without protein)            :: 36
            Pseudo Genes (ambiguous residues) :: 0 of 36
            Pseudo Genes (frameshifted)       :: 2 of 36
            Pseudo Genes (incomplete)         :: 32 of 36
            Pseudo Genes (internal stop)      :: 2 of 36
            ##Genome-Annotation-Data-END##
FEATURES             Location/Qualifiers
     source          1..2029
                     /organism="Micropruina sp."
                     /mol_type="genomic DNA"
                     /submitter_seqid="NODE_22500_length_2029_cov_3.258865"
                     /isolate="AMR_MDS_2195"
                     /isolation_source="Activated sludge of municipal
                     wastewater treatment plants"
                     /db_xref="taxon:2737536"
                     /environmental_sample
                     /geo_loc_name="Canada"
                     /lat_lon="49.25 N 123.1 W"
                     /collection_date="2015-06-16"
                     /metagenome_source="wastewater metagenome"
                     /note="metagenomic"
     gene            complement(<1..1076)
                     /locus_tag="PLF56_13565"
     CDS             complement(<1..1076)
                     /locus_tag="PLF56_13565"
                     /inference="COORDINATES: ab initio
                     prediction:GeneMarkS-2+"
                     /note="Derived by automated computational analysis using
                     gene prediction method: GeneMarkS-2+."
                     /codon_start=1
                     /transl_table=11
                     /product="hypothetical protein"
                     /protein_id="HOQ54627.1"
                     /translation="MMPTPAKSHGCTWHWLSDQDLPEINGLVAAEEHFGDTTHHHDLN
                     GLQRAVSDNGVRRTQTGVVLRKPSGTLIAYAWLRLPQSGEPDYRLHLHGGCHPAWRDE
                     GVQDTLMRWQVERGIEWYLETVPDAADHLNRLELSMLVSAGNHFLAETLPNQGFRPQR
                     WYHALRRPLSPPLAGVELPGVAFEQFGPDVSEAVRQLYNATVASPSDRLEPDAWLWGL
                     QSAGIRDDLSWVAVHAGGPIGWVLNATTDLAGEQAGWTEYLGAARHWRNRGLYSALLA
                     RSHESFLDAGLHTAGIGVETDSDQGARPYLELGYQPIDAMVWYVHHPSLDTIGAAQAE
                     EQAERDLDERAFQVGHHQAQEGGDR"
     gene            complement(1123..1851)
                     /gene="priA"
                     /locus_tag="PLF56_13570"
     CDS             complement(1123..1851)
                     /gene="priA"
                     /locus_tag="PLF56_13570"
                     /EC_number="5.3.1.16"
                     /EC_number="5.3.1.24"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_013161071.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_function: GO:0003949 -
                     1-(5-phosphoribosyl)-5-[(5-
                     phosphoribosylamino)methylideneamino]imidazole-4-
                     carboxamide isomerase activity [Evidence IEA];
                     GO_function: GO:0004640 - phosphoribosylanthranilate
                     isomerase activity [Evidence IEA];
                     GO_process: GO:0000105 - histidine biosynthetic process
                     [Evidence IEA];
                     GO_process: GO:0000162 - tryptophan biosynthetic process
                     [Evidence IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="bifunctional
                     1-(5-phosphoribosyl)-5-((5-
                     phosphoribosylamino)methylideneamino)imidazole-4-
                     carboxamide isomerase/phosphoribosylanthranilate isomerase
                     PriA"
                     /protein_id="HOQ54628.1"
                     /translation="MVTTLTLLPAVDVQNGRAVQLTQGVAGSEKVFGDPLAAAQRWQQ
                     AGAEWLHLVDLDAAFGRGSNAEVIAAITAELSLNVELSGGIRDDASLGRALATGCRRV
                     NIGTAALENPQWCERIIGEHGDRIGIAIDVKGTKLAARGWVREGGDWAEAVDRLSAAG
                     CATFVVTDVQSDGMLTGPNLPLLRAVAARTDAEVIASGGIAQLDDLRALRELVPLGIT
                     GAIIGTALYVGNFTLQDALSAAAG"
BASE COUNT          288 a          713 c          703 g          325 t
ORIGIN      
        1 cgatcgccgc cttcttgtgc ttggtggtgg cccacttgga atgcccgctc atctagatcc
       61 cgttctgctt gctcttcggc ttgcgcggcc ccgattgtat cgaggctcgg atgatggacg
      121 taccagacca tggcgtcgat cggttgatag ccgagctcga ggtacggccg ggcgccctgg
      181 tctgagtcgg tttcgacgcc gattcccgcc gtgtgcaacc ccgcgtccag aaacgattca
      241 tggctgcgcg ccagcagcgc cgaatacagg ccgcggttgc gccagtgccg cgccgcaccc
      301 aggtactcgg tccagccggc ctgttcgccg gccaggtcgg tggtcgcgtt cagcacccag
      361 ccgatgggac ctccggcatg caccgccacc cacgacaggt cgtcgcgaat gcctgcgctc
      421 tgcaggcccc acagccaggc gtccggctcg agccgatcgg acggactcgc cacggtcgcg
      481 ttgtagagct gccggacggc ctcgctgacg tcgggcccga actgttcgaa cgcgaccccc
      541 ggcagctcga caccggccag cggcgggctc agcggacgcc gcagggcgtg gtaccagcgc
      601 tgcggcctga acccttggtt gggcaacgtc tcggccaaga agtggttgcc ggccgaaacc
      661 agcatcgaca actccagccg gttgaggtga tcggcggcat cggggacggt ctcgagatac
      721 cactcgatgc cgcgttcgac ctgccagcgc atcagcgtgt cctggacccc ctcgtcgcgc
      781 caggccgggt ggcagccgcc gtgcaggtgc agccggtagt cgggttcgcc cgactggggc
      841 agccgcagcc aggcgtaggc gatcagggtg ccgctgggtt tgcgcagtac cacgccggtc
      901 tgggtgcggc gcaccccgtt atcgctgacc gcacgctgca ggccgttcag atcgtgatgg
      961 tgggtggtgt cgccgaagtg ctcttcggcg gccacgagac cgttgatctc gggcaggtcc
     1021 tggtcggaca gccagtgcca cgtgcacccg tggctcttgg ccggtgtggg catcatcgcc
     1081 tccgcatagt taccgtcaac tctagtgctc ccgcgcctcg gctcaccctg cagctgcgct
     1141 cagagcgtcc tgaagggtga agttgcccac gtacagggcg gtgccgatga tcgcgccggt
     1201 gataccgagc ggcaccaact cgcgcagcgc ccgcaggtcg tccagttgtg cgatgccgcc
     1261 ggacgcgatc acctcggcgt ccgtgcgtgc cgcgacggcc cgcagcagcg gcagattcgg
     1321 gccggtcagc atgccgtccg actgcacgtc ggtcacgaca aaggtggcgc acccggcggc
     1381 cgacaaccgg tccacggcct cggcccagtc gccgccctcg cgcacccagc cgcgggcggc
     1441 cagcttggtg cccttgacgt cgatcgcgat gccgatccgg tcgccgtgtt cgccgatgat
     1501 gcgttcgcac cactgcggat tctccagggc tgccgtgccg atgttgaccc gccggcagcc
     1561 ggtggccagg gcacgcccaa ggctggcgtc gtcccggatg ccgccgctca actcgacgtt
     1621 gaggctcaac tcggcggtga tggcggcgat cacctcggcg ttcgagccgc gtccgaaggc
     1681 ggcgtcgagg tcgaccaggt gcagccactc ggcgccggcc tgctgccagc gctgggccgc
     1741 ggcgagcggg tcgccgaaca ccttctcgga gccggccacc ccctgggtga gttgaacggc
     1801 ccggccgttc tggacgtcga cggcgggcag cagggtcagg gtggtcacga tcggccagcc
     1861 taaccggtgc cgggtgtcgg ggtggttcgc gccgcccgca cggacgctac ggtcgtggca
     1921 tggaccagca gatccgagag ctgaacgccg ccaagaccgg tggccggacg aactgtcggc
     1981 cgggctgcgc gccgacggcc tggacccgct gaacccggcc ccactgacg
//
LOCUS       DAOSUG010000523         2027 bp    DNA     linear   ENV 10-JUN-2024
DEFINITION  MAG TPA_asm: Micropruina sp. isolate AMR_MDS_2195
            NODE_22533_length_2027_cov_3.385903, whole genome shotgun sequence.
ACCESSION   DAOSUG010000523 DAOSUG010000000
VERSION     DAOSUG010000523.1
DBLINK      BioProject: PRJNA925477
            BioSample: SAMN32864262
            Sequence Read Archive: SRR4236673
KEYWORDS    WGS; ENV; Metagenome Assembled Genome; MAG; Third Party Data; TPA;
            TPA:assembly.
SOURCE      Micropruina sp. (wastewater metagenome)
  ORGANISM  Micropruina sp.
            Bacteria; Bacillati; Actinomycetota; Actinomycetes;
            Propionibacteriales; Nocardioidaceae; Micropruina.
REFERENCE   1  (bases 1 to 2027)
  AUTHORS   Abdulkadir,N., Saraiva,J.P., Zhang,J., Stolte,S., Gillor,O.,
            Harms,H. and Rocha,U.
  TITLE     Genome-centric analyses of 165 metagenomes show that mobile genetic
            elements are crucial for the transmission of antimicrobial
            resistance genes to pathogens in activated sludge and wastewater
  JOURNAL   Microbiol Spectr 12 (3), e0291823 (2024)
   PUBMED   38289113
REFERENCE   2  (bases 1 to 2027)
  AUTHORS   Abdulkadir,N.
  TITLE     Direct Submission
  JOURNAL   Submitted (24-MAR-2023) Environmental Microbiology, Helmholtz
            Center for Environmental Research -UFZ, Permoserstr 15, Leipzig
            04318, Germany
COMMENT     The annotation was added by the NCBI Prokaryotic Genome Annotation
            Pipeline (PGAP). Information about PGAP can be found here:
            https://www.ncbi.nlm.nih.gov/genome/annotation_prok/
            
            ##Genome-Assembly-Data-START##
            Assembly Method        :: SPAdes v. 3.11.1
            Genome Representation  :: Full
            Expected Final Version :: Yes
            Genome Coverage        :: Not Applicable
            Sequencing Technology  :: Illumina MiSeq
            ##Genome-Assembly-Data-END##
            
            ##Genome-Annotation-Data-START##
            Annotation Provider               :: NCBI
            Annotation Date                   :: 05/04/2023 04:57:10
            Annotation Pipeline               :: NCBI Prokaryotic Genome
                                                 Annotation Pipeline (PGAP)
            Annotation Method                 :: Best-placed reference protein
                                                 set; GeneMarkS-2+
            Annotation Software revision      :: 6.5
            Features Annotated                :: Gene; CDS; rRNA; tRNA; ncRNA
            Genes (total)                     :: 2,776
            CDSs (total)                      :: 2,750
            Genes (coding)                    :: 2,714
            CDSs (with protein)               :: 2,714
            Genes (RNA)                       :: 26
            tRNAs                             :: 24
            ncRNAs                            :: 2
            Pseudo Genes (total)              :: 36
            CDSs (without protein)            :: 36
            Pseudo Genes (ambiguous residues) :: 0 of 36
            Pseudo Genes (frameshifted)       :: 2 of 36
            Pseudo Genes (incomplete)         :: 32 of 36
            Pseudo Genes (internal stop)      :: 2 of 36
            ##Genome-Annotation-Data-END##
FEATURES             Location/Qualifiers
     source          1..2027
                     /organism="Micropruina sp."
                     /mol_type="genomic DNA"
                     /submitter_seqid="NODE_22533_length_2027_cov_3.385903"
                     /isolate="AMR_MDS_2195"
                     /isolation_source="Activated sludge of municipal
                     wastewater treatment plants"
                     /db_xref="taxon:2737536"
                     /environmental_sample
                     /geo_loc_name="Canada"
                     /lat_lon="49.25 N 123.1 W"
                     /collection_date="2015-06-16"
                     /metagenome_source="wastewater metagenome"
                     /note="metagenomic"
     gene            <1..681
                     /locus_tag="PLF56_13575"
     CDS             <1..681
                     /locus_tag="PLF56_13575"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_008363323.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="Fic family protein"
                     /protein_id="HOQ54629.1"
                     /translation="IVQAGLAHAQFETILPFTDGTGRVGRALIHTVLTRRGLTTEAVL
                     PVSLVLSTMRSDYVAGLTAYRHSDAVGSVGFHAARAAWLRVFASAVLTATEQAGRLAA
                     DLVNLRTVWDERLTRARSSSGKVRGVRSDSATALIVRDLPATPVLSSATVQRIHGVSH
                     VAADRALAELVAADILHPQERRAARYYQARDVLDLVTVTERRLASTKFDTRTSPPNRP
                     VPARQSRT"
     gene            complement(945..1020)
                     /locus_tag="PLF56_13580"
     tRNA            complement(945..1020)
                     /locus_tag="PLF56_13580"
                     /product="tRNA-Ala"
                     /inference="COORDINATES: profile:tRNAscan-SE:2.0.12"
                     /note="Derived by automated computational analysis using
                     gene prediction method: tRNAscan-SE."
                     /anticodon=(pos:complement(985..987),aa:Ala,seq:cgc)
     gene            complement(1084..1818)
                     /locus_tag="PLF56_13585"
     CDS             complement(1084..1818)
                     /locus_tag="PLF56_13585"
                     /inference="COORDINATES: ab initio
                     prediction:GeneMarkS-2+"
                     /note="Derived by automated computational analysis using
                     gene prediction method: GeneMarkS-2+."
                     /codon_start=1
                     /transl_table=11
                     /product="hypothetical protein"
                     /protein_id="HOQ54630.1"
                     /translation="MTGVIFGVIAAAWLVYLVPYFLKRQGDPALDELDPAVPFSATVT
                     IVRRGASLDVAEAGTATVSTPLTRRAALYELEQIDRQAATRRRRVLVVLTAALLAVSV
                     PTALGYLMWWAPIIPAGLLVLFLVVARFSVRVMRRDLDERARQLREAGDEQTVAIAVV
                     ASADDNAEASVELTAPVTKPGSLWDPIPIVAPTYVSKPLAPRTVRTIDLSAPTPTAGG
                     VPVTADPLPVERPETDEGEGRRAVGE"
BASE COUNT          345 a          745 c          639 g          298 t
ORIGIN      
        1 atcgtgcagg ccgggctcgc gcacgcccag ttcgagacca tcctcccgtt taccgacggc
       61 accggacgcg tcggccgcgc cctcatccac acagttctca cccgccgcgg gctcaccacc
      121 gaggcggtcc ttcccgtgag cctcgtgctc tccaccatgc ggagcgacta cgtcgctggc
      181 ctgaccgcct atcggcactc tgacgccgtc ggctcggtcg gcttccacgc cgcccgagcc
      241 gcctggctgc gtgtgtttgc ctcggcggtc ctcactgcca ccgaacaagc cggacgcctc
      301 gccgccgatc tggtcaactt gcgcacagtt tgggatgaaa gactcacccg cgctcgatcg
      361 agttcgggca aggtgcgtgg cgtccgcagc gactcggcga ccgctctgat cgtgcgcgac
      421 ctgccagcca cgcccgtgct cagcagtgcc accgtgcaac gcatccacgg cgtctcacac
      481 gtcgccgccg accgggcgct cgccgaactg gtcgccgccg acatcctcca cccccaggag
      541 cggcgtgctg cgcgctacta ccaggctcgc gacgtgctcg acctggtcac cgtgaccgaa
      601 cgtcgcttgg ccagcaccaa gttcgacacc cgcaccagcc cgcccaatcg acccgtaccc
      661 gcccgccaga gccggacgtg acctgatccg ctcttccgat gccgatgctt gagtccaggt
      721 cacggagttg gagggcgtgg acctcaagcg ccgaagtcga gacgggctcg ccgcgttcca
      781 gttgccgatc gggtgttgcc actcatccgg gcgatggcga agggcaaggc cggtggggac
      841 gagtcgccga acgtaagggg cacgcgagcc gcgtcagatg tgcctggcgg ccacggtgcg
      901 aatgacgaaa caggagaact ggctagccag cgcaagattt ctaatggtgg agctaagggg
      961 attcgaaccc ctgaccttct cattgcgaac gagacgcgct accaactgcg ccatagcccc
     1021 atgctctgcg agcacatcga ctctagcaaa caaccgccct gcgttcacct tgacggtgga
     1081 ggctcactcg ccgacggcgc ggcggccttc gccctcgtcg gtctcaggac gttccaccgg
     1141 caacggatcg gccgtcaccg gcacgccgcc ggccgttggt gtcggcgccg acagatcgat
     1201 cgtacggacg gtgcgcggcg cgagcggctt cgagacgtac gtgggggcga cgatcgggat
     1261 gggatcccac agcgatcccg gcttcgtcac cggagcggtc agttcgacgg atgcctcggc
     1321 gttgtcgtcg gccgaggcca ccacagcgat cgccacggtc tgctcgtcgc cggcctcacg
     1381 cagttgtcgg gcgcgctcgt ccagatcgcg acgcatcacc cgcacgctga atcgcgccac
     1441 caccagaaac agcaccagca ggccggccgg aatgatcggc gcccaccaca tcaggtaccc
     1501 cagcgccgtg ggaacgctca ccgcgagcag tgccgcggtg agcaccacca gcacccggcg
     1561 ccgacgggtc gcggcctgac gatcgatctg ctcgagctcg tacagcgcag cacggcgggt
     1621 cagcggggtc gacacggtgg ccgtgccggc ctcggccaca tcgagcgacg caccgcgtcg
     1681 aacgatcgtc accgtggccg agaacggaac cgccgggtcg agttcgtcca gcgccgggtc
     1741 gccttgtcgc ttcagaaaat agggcaccag gtacaccagc cacgccgcgg cgatcacgcc
     1801 gaaaatcact cccgtcaagc ccacacccag acgttacggg gggccgccac ccagccgcgg
     1861 caggcgcccc ggagtgtcgc ttgatcaggc gaaatgcaca gcccggatcc gggttcagcc
     1921 ccggtcggac ccggtgccgt caacgcgggc gagcagcccg gggcccacct cttcggcatg
     1981 cagggcgaac atctcgtgat cacgccagtc gccgtccacg tgcatgt
//
LOCUS       DAOSUG010000524         2026 bp    DNA     linear   ENV 10-JUN-2024
DEFINITION  MAG TPA_asm: Micropruina sp. isolate AMR_MDS_2195
            NODE_22546_length_2026_cov_4.040081, whole genome shotgun sequence.
ACCESSION   DAOSUG010000524 DAOSUG010000000
VERSION     DAOSUG010000524.1
DBLINK      BioProject: PRJNA925477
            BioSample: SAMN32864262
            Sequence Read Archive: SRR4236673
KEYWORDS    WGS; ENV; Metagenome Assembled Genome; MAG; Third Party Data; TPA;
            TPA:assembly.
SOURCE      Micropruina sp. (wastewater metagenome)
  ORGANISM  Micropruina sp.
            Bacteria; Bacillati; Actinomycetota; Actinomycetes;
            Propionibacteriales; Nocardioidaceae; Micropruina.
REFERENCE   1  (bases 1 to 2026)
  AUTHORS   Abdulkadir,N., Saraiva,J.P., Zhang,J., Stolte,S., Gillor,O.,
            Harms,H. and Rocha,U.
  TITLE     Genome-centric analyses of 165 metagenomes show that mobile genetic
            elements are crucial for the transmission of antimicrobial
            resistance genes to pathogens in activated sludge and wastewater
  JOURNAL   Microbiol Spectr 12 (3), e0291823 (2024)
   PUBMED   38289113
REFERENCE   2  (bases 1 to 2026)
  AUTHORS   Abdulkadir,N.
  TITLE     Direct Submission
  JOURNAL   Submitted (24-MAR-2023) Environmental Microbiology, Helmholtz
            Center for Environmental Research -UFZ, Permoserstr 15, Leipzig
            04318, Germany
COMMENT     The annotation was added by the NCBI Prokaryotic Genome Annotation
            Pipeline (PGAP). Information about PGAP can be found here:
            https://www.ncbi.nlm.nih.gov/genome/annotation_prok/
            
            ##Genome-Assembly-Data-START##
            Assembly Method        :: SPAdes v. 3.11.1
            Genome Representation  :: Full
            Expected Final Version :: Yes
            Genome Coverage        :: Not Applicable
            Sequencing Technology  :: Illumina MiSeq
            ##Genome-Assembly-Data-END##
            
            ##Genome-Annotation-Data-START##
            Annotation Provider               :: NCBI
            Annotation Date                   :: 05/04/2023 04:57:10
            Annotation Pipeline               :: NCBI Prokaryotic Genome
                                                 Annotation Pipeline (PGAP)
            Annotation Method                 :: Best-placed reference protein
                                                 set; GeneMarkS-2+
            Annotation Software revision      :: 6.5
            Features Annotated                :: Gene; CDS; rRNA; tRNA; ncRNA
            Genes (total)                     :: 2,776
            CDSs (total)                      :: 2,750
            Genes (coding)                    :: 2,714
            CDSs (with protein)               :: 2,714
            Genes (RNA)                       :: 26
            tRNAs                             :: 24
            ncRNAs                            :: 2
            Pseudo Genes (total)              :: 36
            CDSs (without protein)            :: 36
            Pseudo Genes (ambiguous residues) :: 0 of 36
            Pseudo Genes (frameshifted)       :: 2 of 36
            Pseudo Genes (incomplete)         :: 32 of 36
            Pseudo Genes (internal stop)      :: 2 of 36
            ##Genome-Annotation-Data-END##
FEATURES             Location/Qualifiers
     source          1..2026
                     /organism="Micropruina sp."
                     /mol_type="genomic DNA"
                     /submitter_seqid="NODE_22546_length_2026_cov_4.040081"
                     /isolate="AMR_MDS_2195"
                     /isolation_source="Activated sludge of municipal
                     wastewater treatment plants"
                     /db_xref="taxon:2737536"
                     /environmental_sample
                     /geo_loc_name="Canada"
                     /lat_lon="49.25 N 123.1 W"
                     /collection_date="2015-06-16"
                     /metagenome_source="wastewater metagenome"
                     /note="metagenomic"
     gene            complement(255..758)
                     /locus_tag="PLF56_13590"
     CDS             complement(255..758)
                     /locus_tag="PLF56_13590"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_015069873.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_process: GO:0009116 - nucleoside metabolic process
                     [Evidence IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="phosphoribosyltransferase family protein"
                     /protein_id="HOQ54631.1"
                     /translation="MTAYHAEASDTDGKEVLTWQAFGDATRELAAAVAASGFRAEVIV
                     AVARGGLLAAGALAYALGVKLADAMNVEFYTDVATTLPDPVLLAPLLDIDSIAGRRVL
                     VVDDVADSGRTLQLVLDILLAHGAEARTAVLYLKPTSIHRVDFAWKTTDAWIVFPWSA
                     LPPVTAG"
     gene            complement(929..>2026)
                     /locus_tag="PLF56_13595"
     CDS             complement(929..>2026)
                     /locus_tag="PLF56_13595"
                     /inference="COORDINATES: protein motif:HMM:NF037018.2"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="DUF3372 domain-containing protein"
                     /protein_id="HOQ54632.1"
                     /translation="KGSLLEYRIVARDLQGRYSATSTYAFVGDPASAPSTTTNDPPEK
                     QPGSVAVAGSFNAAVGCAADWSPACAQVQLKLDSEDQIWKTTLNVPKGDYDYKAALNG
                     NWDENYGAGGARNGSNINFKPPGGEVSFYYDHATHWVTNSAQGPIITAPGSFQSELGC
                     SADWKPDCMRPWLQDPDGDGTYTWASVKIPKGTYEFKVAHGLSFDQSYPADNVTLTVP
                     ANDMLVTINYVLATHQVSVAVTQAVSKPDLSVAKATLIRDDLIAYPGNALPLGADPRA
                     YSWRLHWSAKGGLAIDSDGVTGGQVVTLTRDWKGLPAGLLTAHPKLAGSIALRVDATT
                     AARMKSILAGQVAVAMYDDQGRLVDATGLKA"
BASE COUNT          325 a          688 c          677 g          336 t
ORIGIN      
        1 gtcggggccc gcactcgtca tcgcgggctt gccccttgat cacgtcctgc gccccgggat
       61 gacgggggtc acgatgtgca cggaagcgag gatcaaccca caggtcgaaa cccgcacctg
      121 cgacagcggg ctcgacgtgc ggtggggcac aagacacgcc ccaacgcgtg cctgattgac
      181 gtggctgacg gaaacgtgcg atccgatgct cgacagcgtg agcccgagca gcccgggcac
      241 tcagctgaac gacctcaacc ggccgtgacc ggagggagtg ccgaccaggg aaaaacgatc
      301 caggcgtcgg tggtcttcca cgcgaaatcg acccggtgga tcgaggtggg cttgaggtac
      361 agcaccgccg tgcgcgcctc ggcgccgtgc gcgagcagga tgtccagcac cagttgcagg
      421 gtgcgcccgg agtcggccac gtcgtcgacc accagcaccc ggcggccggc gatgctgtcg
      481 atgtcgagca agggcgcgag cagtacgggg tcgggcagag tggtcgcgac gtcggtgtag
      541 aactccacgt tcatcgcgtc cgccagcttc acgccgagcg cgtacgcgag cgcaccggcg
      601 gccagcagcc cgccccgcgc gaccgcaacg atcacctcgg cgcgaaaacc gctcgccgcc
      661 accgcggccg ccaactcgcg cgtggcgtca ccgaatgcct gccaggtgag cacttctttg
      721 ccgtcggtgt cggacgcttc tgcgtggtac gccgtcatgc ccgaacccta agcaggcgcc
      781 gatcaacgtg caatccgcgc gccgcgctcg tcgaaacccc tggtcgctcg ggcctcgaca
      841 agctcgacca gcgctggctc agcgtttcgc tcacgggcac cccgacgcat tcactccgtt
      901 tcagccctgg ccggacccca tggaccggct acgccttcaa tccggtggcg tcgaccaatc
      961 ggccctggtc gtcgtacatc gcgacggcga cctggcccgc caggatcgac ttcattctgg
     1021 ccgccgtcgt ggcgtcgacc cgtagcgcga tcgaaccggc cagcttggga tgcgctgtca
     1081 gcaacccggc cggcaacccc ttccagtctc tggtcaaggt gacgacctgg ccaccagtga
     1141 cgccgtcgga gtcgatcgcc aggccaccct tggccgacca atgcagacgc cagctgtagg
     1201 cgcgggggtc ggcaccgagt ggcagcgcgt tgcccgggta ggcgatcagg tcgtcgcgaa
     1261 tcaacgtcgc cttcgccacg ctcaggtcgg gcttcgacac cgcctgggtg accgccaccg
     1321 agacctggtg cgtggccagc acgtagttga tcgtgaccag catgtcgttc gccggcacgg
     1381 tgagcgtgac gttgtcggcc gggtaggact gatcgaagct cagtccgtgt gccaccttga
     1441 attcgtaggt gcccttcgga atcttgaccg aggcccaggt gtaggtgccg tcgccgtccg
     1501 ggtcctgcaa ccacggccgc atgcagtcag gcttccagtc cgccgagcag cccagctcgc
     1561 tttggaaact gccgggcgcg gtgatgatcg gtccctgtgc actgttggtc acccagtggg
     1621 tggcatggtc gtagtagaag ctgacttcgc cgccgggcgg cttgaagttg atgttcgacc
     1681 cgttgcgggc accaccggcg ccgtagttct cgtcccagtt gccgttgagc gccgccttgt
     1741 agtcgtagtc gcccttggga acgttcaggg tggtcttcca gatctgatcc tctgagtcga
     1801 gcttcagctg cacttgggcg caggcgggag accaatcggc tgcgcagccg acggccgcat
     1861 tgaacgagcc cgccaccgcg acggagccgg gctgcttctc gggcggatcg ttggtcgtgg
     1921 tgctgggcgc ggaggccggg tcacccacga acgcgtaggt cgaggtggcc gagtagcggc
     1981 cctgcaggtc cctggccacg atgcggtact ccagcagcga cccctt
//
LOCUS       DAOSUG010000525         1986 bp    DNA     linear   ENV 10-JUN-2024
DEFINITION  MAG TPA_asm: Micropruina sp. isolate AMR_MDS_2195
            NODE_23224_length_1986_cov_4.526152, whole genome shotgun sequence.
ACCESSION   DAOSUG010000525 DAOSUG010000000
VERSION     DAOSUG010000525.1
DBLINK      BioProject: PRJNA925477
            BioSample: SAMN32864262
            Sequence Read Archive: SRR4236673
KEYWORDS    WGS; ENV; Metagenome Assembled Genome; MAG; Third Party Data; TPA;
            TPA:assembly.
SOURCE      Micropruina sp. (wastewater metagenome)
  ORGANISM  Micropruina sp.
            Bacteria; Bacillati; Actinomycetota; Actinomycetes;
            Propionibacteriales; Nocardioidaceae; Micropruina.
REFERENCE   1  (bases 1 to 1986)
  AUTHORS   Abdulkadir,N., Saraiva,J.P., Zhang,J., Stolte,S., Gillor,O.,
            Harms,H. and Rocha,U.
  TITLE     Genome-centric analyses of 165 metagenomes show that mobile genetic
            elements are crucial for the transmission of antimicrobial
            resistance genes to pathogens in activated sludge and wastewater
  JOURNAL   Microbiol Spectr 12 (3), e0291823 (2024)
   PUBMED   38289113
REFERENCE   2  (bases 1 to 1986)
  AUTHORS   Abdulkadir,N.
  TITLE     Direct Submission
  JOURNAL   Submitted (24-MAR-2023) Environmental Microbiology, Helmholtz
            Center for Environmental Research -UFZ, Permoserstr 15, Leipzig
            04318, Germany
COMMENT     The annotation was added by the NCBI Prokaryotic Genome Annotation
            Pipeline (PGAP). Information about PGAP can be found here:
            https://www.ncbi.nlm.nih.gov/genome/annotation_prok/
            
            ##Genome-Assembly-Data-START##
            Assembly Method        :: SPAdes v. 3.11.1
            Genome Representation  :: Full
            Expected Final Version :: Yes
            Genome Coverage        :: Not Applicable
            Sequencing Technology  :: Illumina MiSeq
            ##Genome-Assembly-Data-END##
            
            ##Genome-Annotation-Data-START##
            Annotation Provider               :: NCBI
            Annotation Date                   :: 05/04/2023 04:57:10
            Annotation Pipeline               :: NCBI Prokaryotic Genome
                                                 Annotation Pipeline (PGAP)
            Annotation Method                 :: Best-placed reference protein
                                                 set; GeneMarkS-2+
            Annotation Software revision      :: 6.5
            Features Annotated                :: Gene; CDS; rRNA; tRNA; ncRNA
            Genes (total)                     :: 2,776
            CDSs (total)                      :: 2,750
            Genes (coding)                    :: 2,714
            CDSs (with protein)               :: 2,714
            Genes (RNA)                       :: 26
            tRNAs                             :: 24
            ncRNAs                            :: 2
            Pseudo Genes (total)              :: 36
            CDSs (without protein)            :: 36
            Pseudo Genes (ambiguous residues) :: 0 of 36
            Pseudo Genes (frameshifted)       :: 2 of 36
            Pseudo Genes (incomplete)         :: 32 of 36
            Pseudo Genes (internal stop)      :: 2 of 36
            ##Genome-Annotation-Data-END##
FEATURES             Location/Qualifiers
     source          1..1986
                     /organism="Micropruina sp."
                     /mol_type="genomic DNA"
                     /submitter_seqid="NODE_23224_length_1986_cov_4.526152"
                     /isolate="AMR_MDS_2195"
                     /isolation_source="Activated sludge of municipal
                     wastewater treatment plants"
                     /db_xref="taxon:2737536"
                     /environmental_sample
                     /geo_loc_name="Canada"
                     /lat_lon="49.25 N 123.1 W"
                     /collection_date="2015-06-16"
                     /metagenome_source="wastewater metagenome"
                     /note="metagenomic"
     gene            <1..346
                     /locus_tag="PLF56_13600"
     CDS             <1..346
                     /locus_tag="PLF56_13600"
                     /inference="COORDINATES: protein motif:HMM:NF025910.2"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=2
                     /transl_table=11
                     /product="tetratricopeptide repeat protein"
                     /protein_id="HOQ54633.1"
                     /translation="ETTMYDWQAALLAFDTRDYKVAAERFEKILEAQPGHTDAREYLA
                     RTHYLRASLGPAEREVRTILEADPTNEYVTLLLARVLERQSRHGEAAGVRRVLVALTG
                     DERHGPTHQALA"
     gene            715..1785
                     /locus_tag="PLF56_13605"
     CDS             715..1785
                     /locus_tag="PLF56_13605"
                     /inference="COORDINATES: protein motif:HMM:NF033542.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_function: GO:0004803 - transposase activity [Evidence
                     IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="IS110 family transposase"
                     /protein_id="HOQ54634.1"
                     /translation="MAIVADTYTYVVGVDTHAATHHYAIVETRSGGQIAHGKFPTHAA
                     GLLRAVDWISRRTSVDDNPAVDQVLISMEGTGSYGAQAAELLAARGYRVVDAPSPKRA
                     RGSGKNDHIDALVAARGALPKDSERLADRRAGHTRAALQILLTARDSMRRDRTRSKNQ
                     LTALLRTHNLDIDARKGINKVHISLIAGWRKRPTDTTLARIARTEAHRLATRIHTLDA
                     DLDSNEKAINALVRELEPTLLDLPGLGPINAAIVLAVWSHPGRIHDEAGFAKIGGVCP
                     LEISSGNSKDHRLNRTGDRQLNSALNSIANTRMRHDETTKAYVKKRKQQGQTKPRIRR
                     CLKRYIARQIFRHLNTTTLDKT"
BASE COUNT          379 a          731 c          603 g          273 t
ORIGIN      
        1 cgagaccacc atgtacgact ggcaggcggc gttgctcgcc ttcgacactc gcgactacaa
       61 ggtcgccgcg gagcgcttcg agaagatcct ggaggcgcaa ccgggccaca ccgatgcgcg
      121 cgagtacctg gcccgcacgc actacctgcg ggcctcgctg ggccccgccg agcgtgaggt
      181 gcggacgatt ctcgaggccg acccgaccaa cgagtacgtc acgctgctgc tggcccgggt
      241 gctcgagcgg cagagccggc acggcgaggc cgccggcgtc cgtcgggtgc tggtcgccct
      301 gaccggcgac gagcggcacg gcccgacgca ccaggccctg gcctgagccc gtccgctgat
      361 agagcttgtc gaaaccccct ggccggggtc tcgacaagct gtgagagttg gtggtggctg
      421 gcctggggct agctggcagg gtggtgggac cggttgttcg tgtgcgggtc gtgacccttc
      481 gacaagctga ccgtccgcaa ttgtgcgggg tgtcttggtc gagatctgtc cggctcgtgc
      541 acggagaacc ggccgaccat cccggcccac acgttggcct gatagaagcc cgtcccaccg
      601 ctttggcggg gtgaccgatc acagtaacgc cacgggtgac tctttccccg ggatggccgc
      661 gccggttcga ccggctcgat gcccaacatc ccgaaacagg aggctcagac accgatggcc
      721 atcgttgcag atacctacac ctatgtggtg ggtgtggaca cccacgccgc cactcatcat
      781 tacgcgatcg tggagacccg tagcggcggc cagatcgccc acgggaagtt ccccacccat
      841 gccgcgggct tgttgcgggc cgtggattgg atcagccgcc gcaccagtgt tgacgacaac
      901 ccagctgtcg accaggtgct gatcagcatg gaaggcaccg gcagttacgg cgcccaagca
      961 gccgagctgc tcgccgcacg cggttaccgg gtcgtcgacg caccctcgcc gaaacgggct
     1021 cgtggcagcg gcaagaacga ccacatcgat gccctggtcg ctgcccgtgg cgcgttaccc
     1081 aaggacagcg aacggctcgc cgaccgacgc gccggccata cccgtgccgc gctgcagatc
     1141 ctgctcaccg cccgcgacag catgcgacgc gaccggaccc gttccaagaa ccagctcacc
     1201 gcgctgctac gcacccacaa cctcgacatc gatgcccgca agggaatcaa caaggtccac
     1261 atcagcctca ttgcgggctg gcgcaagcgg cccaccgaca ccaccctggc ccggatcgcc
     1321 cgcaccgaag cgcaccgcct cgccacccgc atccacaccc tggacgccga cctcgacagc
     1381 aacgagaaag ccatcaacgc cctcgtgcgt gaactcgaac caaccctgct cgacctgccc
     1441 ggcctcggac ccatcaacgc cgccattgtg ctcgccgtct ggtctcaccc cggccgcatc
     1501 catgacgaag ccggcttcgc caagatcggc ggcgtctgcc ccctggagat ctcctcgggc
     1561 aactccaagg atcatcgcct caaccgcacc ggcgaccgac aactcaactc agccctgaac
     1621 tccatcgcga acacccgcat gcgacacgac gaaaccacca aggcctacgt caagaaacgc
     1681 aaacagcaag gccaaaccaa accccgaatc cgacgctgcc tcaagcgcta catcgcccgc
     1741 caaatcttcc gacacctcaa cacgaccacc cttgacaaga catagaagcg tctcgaccga
     1801 cgaccggcga agggttcagc cgggggtcgg acgcaccacg ggggtccggg gcgaccacgg
     1861 caccgagccg aggtcacgca cgccgtcggg caggtcggcc agggtctggc tgctcaccca
     1921 caggcaggcg ccgtcggagt cggtgagcag ttcgtcgaag ccggcgccgc ggcccaccag
     1981 gtgcgc
//
LOCUS       DAOSUG010000526         1984 bp    DNA     linear   ENV 10-JUN-2024
DEFINITION  MAG TPA_asm: Micropruina sp. isolate AMR_MDS_2195
            NODE_23257_length_1984_cov_4.299119, whole genome shotgun sequence.
ACCESSION   DAOSUG010000526 DAOSUG010000000
VERSION     DAOSUG010000526.1
DBLINK      BioProject: PRJNA925477
            BioSample: SAMN32864262
            Sequence Read Archive: SRR4236673
KEYWORDS    WGS; ENV; Metagenome Assembled Genome; MAG; Third Party Data; TPA;
            TPA:assembly.
SOURCE      Micropruina sp. (wastewater metagenome)
  ORGANISM  Micropruina sp.
            Bacteria; Bacillati; Actinomycetota; Actinomycetes;
            Propionibacteriales; Nocardioidaceae; Micropruina.
REFERENCE   1  (bases 1 to 1984)
  AUTHORS   Abdulkadir,N., Saraiva,J.P., Zhang,J., Stolte,S., Gillor,O.,
            Harms,H. and Rocha,U.
  TITLE     Genome-centric analyses of 165 metagenomes show that mobile genetic
            elements are crucial for the transmission of antimicrobial
            resistance genes to pathogens in activated sludge and wastewater
  JOURNAL   Microbiol Spectr 12 (3), e0291823 (2024)
   PUBMED   38289113
REFERENCE   2  (bases 1 to 1984)
  AUTHORS   Abdulkadir,N.
  TITLE     Direct Submission
  JOURNAL   Submitted (24-MAR-2023) Environmental Microbiology, Helmholtz
            Center for Environmental Research -UFZ, Permoserstr 15, Leipzig
            04318, Germany
COMMENT     The annotation was added by the NCBI Prokaryotic Genome Annotation
            Pipeline (PGAP). Information about PGAP can be found here:
            https://www.ncbi.nlm.nih.gov/genome/annotation_prok/
            
            ##Genome-Assembly-Data-START##
            Assembly Method        :: SPAdes v. 3.11.1
            Genome Representation  :: Full
            Expected Final Version :: Yes
            Genome Coverage        :: Not Applicable
            Sequencing Technology  :: Illumina MiSeq
            ##Genome-Assembly-Data-END##
            
            ##Genome-Annotation-Data-START##
            Annotation Provider               :: NCBI
            Annotation Date                   :: 05/04/2023 04:57:10
            Annotation Pipeline               :: NCBI Prokaryotic Genome
                                                 Annotation Pipeline (PGAP)
            Annotation Method                 :: Best-placed reference protein
                                                 set; GeneMarkS-2+
            Annotation Software revision      :: 6.5
            Features Annotated                :: Gene; CDS; rRNA; tRNA; ncRNA
            Genes (total)                     :: 2,776
            CDSs (total)                      :: 2,750
            Genes (coding)                    :: 2,714
            CDSs (with protein)               :: 2,714
            Genes (RNA)                       :: 26
            tRNAs                             :: 24
            ncRNAs                            :: 2
            Pseudo Genes (total)              :: 36
            CDSs (without protein)            :: 36
            Pseudo Genes (ambiguous residues) :: 0 of 36
            Pseudo Genes (frameshifted)       :: 2 of 36
            Pseudo Genes (incomplete)         :: 32 of 36
            Pseudo Genes (internal stop)      :: 2 of 36
            ##Genome-Annotation-Data-END##
FEATURES             Location/Qualifiers
     source          1..1984
                     /organism="Micropruina sp."
                     /mol_type="genomic DNA"
                     /submitter_seqid="NODE_23257_length_1984_cov_4.299119"
                     /isolate="AMR_MDS_2195"
                     /isolation_source="Activated sludge of municipal
                     wastewater treatment plants"
                     /db_xref="taxon:2737536"
                     /environmental_sample
                     /geo_loc_name="Canada"
                     /lat_lon="49.25 N 123.1 W"
                     /collection_date="2015-06-16"
                     /metagenome_source="wastewater metagenome"
                     /note="metagenomic"
     gene            complement(<1..1033)
                     /locus_tag="PLF56_13610"
     CDS             complement(<1..1033)
                     /locus_tag="PLF56_13610"
                     /inference="COORDINATES: protein motif:HMM:NF012711.2"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="AMP-binding protein"
                     /protein_id="HOQ54635.1"
                     /translation="MTLDNPTTPAQDPPQTASPIPCPPLPDEPLTAALARAAASRGDR
                     AALDFLGRTTTYAQLADQVNRAAGALVALGVQPGDRVAIALPNCSTHLVAFYAALRIG
                     AVVVENNPTYSAAELVHQLADSGATVALLWTKAVPGVLAAQSDTSLRHVIAVDLAHDL
                     PLMKRLALRLPIKKARRTAEAMRGPVPAGVPLWHKVHRAAEPLPADHPEPLSSDIALL
                     QYTGGTTGTPKAATLSHANLVANAVQGAAWTGHRTGTETVFGVLPFFHAFGLTLCLSY
                     AIRIAATLVIFPSFDTALVLAAQRRRRGTFLPAVPPMLDRLVTAAESADVSLHSFTHA
                     ISGAMKLPAE"
     gene            complement(1183..>1984)
                     /locus_tag="PLF56_13615"
     CDS             complement(1183..>1984)
                     /locus_tag="PLF56_13615"
                     /inference="COORDINATES: protein motif:HMM:NF012335.2"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=2
                     /transl_table=11
                     /product="zinc-binding dehydrogenase"
                     /protein_id="HOQ54636.1"
                     /translation="PGGQAEYVRVPHADFGPVVLPSDLPDERFLYLSDILPTAWQGVD
                     YADVGADDTLAVVGLGPVGQLAVRSALRRGVGRVIGVDLVPERLAKAASVGVEVVNAA
                     EVDDVAGALRDLTDGRGPDGVLEAVGMEAHGSPVAAAVIGATARMPKPLAKAALENVG
                     IDRLAALKLCIEAVRRGGTVSISGVYGGMADPLPMMTLFDKGLTLRMGQCHVKRWTDE
                     LLDIAQRPGDELGLESLATHRLPLSEAPQAYRMFQQKTDGCLKVVLTP"
BASE COUNT          287 a          692 c          720 g          285 t
ORIGIN      
        1 tctcggcggg cagtttcatc gcaccgctga tcgcgtgggt gaagctgtgc agcgacacgt
       61 cggcgctttc ggcggcggtg accagccggt cgagcatcgg gggtacggcg ggcaggaacg
      121 tgccccggcg tcggcgctgc gcggccagca ccaaggcggt gtcgaacgac ggaaagatca
      181 ccagcgtggc tgcgatccgg atcgcatacg acaggcacag ggtgagcccg aacgcgtgaa
      241 agaacggcag cacgccgaac acggtctcgg taccggtgcg atgccccgtc catgccgcac
      301 cctgcacggc gttggcgacc aggttggcgt ggctgagcgt cgcagccttg ggagttccag
      361 tggtgccccc ggtgtactgc agcagcgcga tgtcggacga aagcggttcg gggtggtcgg
      421 ccggcagcgg ttctgcggcg cgatgcacct tgtgccacag cggtaccccg gccggcaccg
      481 ggccgcgcat cgcctcggcc gtgcggcgtg ccttcttgat cggcaggcgc aacgcgagcc
      541 gcttcatcag cggaaggtcg tgggccaggt cgacggcgat cacgtgacgc agcgaggtgt
      601 ccgactgtgc ggccagcacg cccggcacgg ccttggtcca cagcagggcg acggtcgcgc
      661 ccgaatcggc caactggtgg acgagttcgg cggcggagta ggtgggattg ttttcgacca
      721 cgacggcgcc gattcgcagg gcggcataga acgcgaccag gtgtgtggag cagttcggca
      781 acgcgatcgc cacccggtca ccgggctgca cgcccagcgc caccagggcc ccggcggccc
      841 ggttcacctg gtcggccagc tgggcatagg tggtggtgcg gcccagaaag tccagcgccg
      901 cccggtcacc gcgggacgcg gccgctcggg cgagggccgc ggtcagcggc tcgtccggaa
      961 ggggtgggca ggggatcggc gaagcggtct gcggcgggtc ctgcgccggc gtggtcgggt
     1021 tgtcgagcgt cacgtcctcc acagtaacct acgcaaccgt aagttggggt gtgtgggggt
     1081 ctcgacaacc gcgaccagcg atggggtttc gacagcttcg accggcggtg agggtgtgga
     1141 cgggccgacc ggccgcactc tcggccacgc gccgacccgg actcaggggg tgagcaccac
     1201 cttgaggcac ccgtccgtct tctgttggaa catccggtat gcctgcggtg cctcgctgag
     1261 cggcagccgg tgcgtggcca gtgactccag ccccagctcg tcgcccggac gctgggcgat
     1321 gtcgagcaac tcgtccgtcc agcgcttgac gtggcactgg cccatccgca gggtcagccc
     1381 cttgtcgaac agcgtcatca tcggcagcgg gtcggccatg ccgccgtaca cgccgctgat
     1441 gctcaccgtg cccccccgcc gcaccgcctc gatgcacagt ttcagcgcgg ccaaccggtc
     1501 gatgcccacg ttttcgaggg cagccttggc cagcggcttc ggcatgcggg ctgtcgctcc
     1561 gatcaccgcg gccgcgaccg ggctgccgtg cgcctccatg ccgacggcct cgaggacgcc
     1621 gtccgggccc cgcccgtcgg tcaggtcgcg cagggcaccg gccacgtcgt cgacctcggc
     1681 ggcgttcacc acttcgacgc cgacggaggc cgctttcgcc agccgttcgg gcaccagatc
     1741 caccccgatc acccggccca cgcctcgcct cagggcgctg cgcaccgcca actggccgac
     1801 cgggccgagg ccgaccacgg ccagggtgtc gtccgcgccg acgtcggcgt agtccacccc
     1861 ctgccaggcg gtcggcagaa tgtcggagag gtacaggaac cgctcgtcgg gcaggtcgga
     1921 cggcagcacc accgggccga agtcggcgtg cggcacccgc acgtactcgg cctggccgcc
     1981 cggc
//
LOCUS       DAOSUG010000527         1973 bp    DNA     linear   ENV 10-JUN-2024
DEFINITION  MAG TPA_asm: Micropruina sp. isolate AMR_MDS_2195
            NODE_23448_length_1973_cov_3.159020, whole genome shotgun sequence.
ACCESSION   DAOSUG010000527 DAOSUG010000000
VERSION     DAOSUG010000527.1
DBLINK      BioProject: PRJNA925477
            BioSample: SAMN32864262
            Sequence Read Archive: SRR4236673
KEYWORDS    WGS; ENV; Metagenome Assembled Genome; MAG; Third Party Data; TPA;
            TPA:assembly.
SOURCE      Micropruina sp. (wastewater metagenome)
  ORGANISM  Micropruina sp.
            Bacteria; Bacillati; Actinomycetota; Actinomycetes;
            Propionibacteriales; Nocardioidaceae; Micropruina.
REFERENCE   1  (bases 1 to 1973)
  AUTHORS   Abdulkadir,N., Saraiva,J.P., Zhang,J., Stolte,S., Gillor,O.,
            Harms,H. and Rocha,U.
  TITLE     Genome-centric analyses of 165 metagenomes show that mobile genetic
            elements are crucial for the transmission of antimicrobial
            resistance genes to pathogens in activated sludge and wastewater
  JOURNAL   Microbiol Spectr 12 (3), e0291823 (2024)
   PUBMED   38289113
REFERENCE   2  (bases 1 to 1973)
  AUTHORS   Abdulkadir,N.
  TITLE     Direct Submission
  JOURNAL   Submitted (24-MAR-2023) Environmental Microbiology, Helmholtz
            Center for Environmental Research -UFZ, Permoserstr 15, Leipzig
            04318, Germany
COMMENT     The annotation was added by the NCBI Prokaryotic Genome Annotation
            Pipeline (PGAP). Information about PGAP can be found here:
            https://www.ncbi.nlm.nih.gov/genome/annotation_prok/
            
            ##Genome-Assembly-Data-START##
            Assembly Method        :: SPAdes v. 3.11.1
            Genome Representation  :: Full
            Expected Final Version :: Yes
            Genome Coverage        :: Not Applicable
            Sequencing Technology  :: Illumina MiSeq
            ##Genome-Assembly-Data-END##
            
            ##Genome-Annotation-Data-START##
            Annotation Provider               :: NCBI
            Annotation Date                   :: 05/04/2023 04:57:10
            Annotation Pipeline               :: NCBI Prokaryotic Genome
                                                 Annotation Pipeline (PGAP)
            Annotation Method                 :: Best-placed reference protein
                                                 set; GeneMarkS-2+
            Annotation Software revision      :: 6.5
            Features Annotated                :: Gene; CDS; rRNA; tRNA; ncRNA
            Genes (total)                     :: 2,776
            CDSs (total)                      :: 2,750
            Genes (coding)                    :: 2,714
            CDSs (with protein)               :: 2,714
            Genes (RNA)                       :: 26
            tRNAs                             :: 24
            ncRNAs                            :: 2
            Pseudo Genes (total)              :: 36
            CDSs (without protein)            :: 36
            Pseudo Genes (ambiguous residues) :: 0 of 36
            Pseudo Genes (frameshifted)       :: 2 of 36
            Pseudo Genes (incomplete)         :: 32 of 36
            Pseudo Genes (internal stop)      :: 2 of 36
            ##Genome-Annotation-Data-END##
FEATURES             Location/Qualifiers
     source          1..1973
                     /organism="Micropruina sp."
                     /mol_type="genomic DNA"
                     /submitter_seqid="NODE_23448_length_1973_cov_3.159020"
                     /isolate="AMR_MDS_2195"
                     /isolation_source="Activated sludge of municipal
                     wastewater treatment plants"
                     /db_xref="taxon:2737536"
                     /environmental_sample
                     /geo_loc_name="Canada"
                     /lat_lon="49.25 N 123.1 W"
                     /collection_date="2015-06-16"
                     /metagenome_source="wastewater metagenome"
                     /note="metagenomic"
     gene            complement(87..434)
                     /locus_tag="PLF56_13620"
     CDS             complement(87..434)
                     /locus_tag="PLF56_13620"
                     /inference="COORDINATES: ab initio
                     prediction:GeneMarkS-2+"
                     /note="Derived by automated computational analysis using
                     gene prediction method: GeneMarkS-2+."
                     /codon_start=1
                     /transl_table=11
                     /product="hypothetical protein"
                     /protein_id="HOQ54637.1"
                     /translation="MISTHTFLQGIHPFAGSGLTVFDPIGDGLTHPVPDGRICQALYF
                     RGGNDTDELIVVALLRDGEVRRLFPIGARSSVHVPLRVVEDVEGGSVLELRLAAPSGL
                     SGTAVIDLGLVEH"
     gene            complement(683..>1973)
                     /locus_tag="PLF56_13625"
     CDS             complement(683..>1973)
                     /locus_tag="PLF56_13625"
                     /inference="COORDINATES: protein motif:HMM:NF012872.2"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=2
                     /transl_table=11
                     /product="molybdopterin oxidoreductase"
                     /protein_id="HOQ54638.1"
                     /translation="ECEPGDSEPADAWFDRVVRWLDQADSPAGAQAGPPPAQTTYVGE
                     PTAAPPPVAANGAAVYSRTSPLVTRLTRNRRLGAPTSTKDVRQFGFATDSDFSYRAGD
                     ALSVRPRNAPDAVTAFLLATGLRGDQVVEVPGVGAAELADACLRHLEIVNVSTAVLGF
                     VNERAGDRDLATLLRAGNTLKLQQWLWGKQLVDVLEQYPVTASAQDWASVLRPLQPRQ
                     YSISSSPLAHPDEVHLTVSAVRFQYQGRQRNGVCSTFLADRGDDAEVPVFMLPTKHFR
                     PPERADVPMIMIGPGTGVAPFRGFLQERRELGHRGPNWLFFGERNSATDFYYADELTE
                     LRDQGVLSELTLAFSRDQREKIYVQDRLREQGARVWSWLADGAHVYVCGDASRMAKDV
                     DKALHDVLCTNAGMSDDAATEYLAGMAKQKRYVRDVY"
BASE COUNT          289 a          658 c          724 g          302 t
ORIGIN      
        1 ccatgccgtt gccgatcacc acgagcctgc gcagggcgac cggccgctcg tcgagccggg
       61 gaggggtggc gtcggtgatc gtcatgtcag tgctccacca ggccgaggtc gatcaccgct
      121 gtgccgctca ggccggacgg cgccgctagg cgcaactcga gcaccgagcc gccctcgacg
      181 tcctcgacca cccgcagcgg cacgtgcacc gacgagcggg cgccgatcgg gaacagccgt
      241 cgcacctcgc cgtcacgcag tagggccacc acgatcagct cgtcggtgtc gttgccgccg
      301 cggaagtaca acgcctggca gatccggccg tccggcaccg ggtgggtgag cccgtcgccg
      361 atcgggtcga acaccgtcag gccgctgccg gcgaacgggt ggatgccctg cagaaaggtg
      421 tgggtggaga tcatcggcgc attcgctttc ggtcaggcgc tcaaccgatt ggcgaagacc
      481 ccgcgcgggg ggctgtcgtt ggaccggccg acgcgtcatt ggggcttgcg tattcattca
      541 agaggggcaa tgtttccgcc gggtggtgcg ccggttacgt tcctttcacg gcacgttcac
      601 agcggccggg gtggggcggt gaggtttcaa ccggtgccgg ggcgcagcgc cgcgaatcag
      661 cccggagcat gcgggatcgg gatcagtaca cgtcgcgcac gtagcgcttc tgcttggcca
      721 tgccggccag gtactcggtg gccgcgtcgt ccgacatgcc cgcattggtg cagagcacgt
      781 cgtgcagcgc cttgtcgacg tccttggcca tccggctggc gtctccgcag acgtagacgt
      841 gggcgccgtc cgcgagccac gaccagacgc gtgcgccctg ctcgcgcagc cggtcctgga
      901 cgtagatctt ctcgcgctgg tcacgtgaga aggccagggt caactcgctc agcacaccct
      961 ggtcacgcag ctcggtgagt tcgtcggcgt agtagaagtc ggtggccgag ttgcgttcgc
     1021 cgaagaacag ccagttgggc ccccggtggc ccagctcgcg acgctcctga agaaaccccc
     1081 ggaagggggc gacgccggtg ccgggaccga tcatgatcat cggcacgtcg gcccgttccg
     1141 gaggccggaa atgcttggtg ggcagcatga acaccggcac ctcggcgtcg tcgccccggt
     1201 cggccagaaa cgtcgagcag accccgttgc gctgtcgtcc ctggtactgg aagcgcaccg
     1261 ccgacacggt caggtgcacc tcgtcaggat gggcgagcgg gctggacgag atcgagtact
     1321 gccggggctg caacgggcgc agcacgctcg cccagtcctg cgccgaagcg gtcaccgggt
     1381 actgctccag cacgtccacc agctgcttgc cccacagcca ttgctgcagc ttcagcgtgt
     1441 tgccggcgcg cagcagggtg gccaggtcgc ggtcgccggc ccgttcgttg acgaaaccga
     1501 gtacggcggt cgagacgttg acgatctcga ggtgccgcag gcaggcgtcg gccagttcgg
     1561 cggcacccac ccccggcacc tcgacgacct ggtcgccgcg caggccggtc gcgagcagaa
     1621 aggccgtgac cgcgtccggt gcgttccggg gccggacgct gagcgcgtcc ccggcccggt
     1681 agctgaagtc gctgtccgtg gcgaaaccga actggcggac gtccttggtc gaggtgggcg
     1741 cgccgagccg gcggttgcgg gtgagccggg tgaccagcgg cgaggtgcgc gagtagacgg
     1801 cagccccgtt ggccgcgacg ggtggcgggg cagccgtggg ttcgccgacg taggtggtct
     1861 gcgcgggtgg tggaccggcc tgggcgccgg ccggcgagtc ggcctggtcg agccagcgga
     1921 ccacccggtc gaaccaggca tcggcgggtt cgctgtcgcc cggttcgcac tcg
//
LOCUS       DAOSUG010000528         1961 bp    DNA     linear   ENV 10-JUN-2024
DEFINITION  MAG TPA_asm: Micropruina sp. isolate AMR_MDS_2195
            NODE_23640_length_1961_cov_4.961175, whole genome shotgun sequence.
ACCESSION   DAOSUG010000528 DAOSUG010000000
VERSION     DAOSUG010000528.1
DBLINK      BioProject: PRJNA925477
            BioSample: SAMN32864262
            Sequence Read Archive: SRR4236673
KEYWORDS    WGS; ENV; Metagenome Assembled Genome; MAG; Third Party Data; TPA;
            TPA:assembly.
SOURCE      Micropruina sp. (wastewater metagenome)
  ORGANISM  Micropruina sp.
            Bacteria; Bacillati; Actinomycetota; Actinomycetes;
            Propionibacteriales; Nocardioidaceae; Micropruina.
REFERENCE   1  (bases 1 to 1961)
  AUTHORS   Abdulkadir,N., Saraiva,J.P., Zhang,J., Stolte,S., Gillor,O.,
            Harms,H. and Rocha,U.
  TITLE     Genome-centric analyses of 165 metagenomes show that mobile genetic
            elements are crucial for the transmission of antimicrobial
            resistance genes to pathogens in activated sludge and wastewater
  JOURNAL   Microbiol Spectr 12 (3), e0291823 (2024)
   PUBMED   38289113
REFERENCE   2  (bases 1 to 1961)
  AUTHORS   Abdulkadir,N.
  TITLE     Direct Submission
  JOURNAL   Submitted (24-MAR-2023) Environmental Microbiology, Helmholtz
            Center for Environmental Research -UFZ, Permoserstr 15, Leipzig
            04318, Germany
COMMENT     The annotation was added by the NCBI Prokaryotic Genome Annotation
            Pipeline (PGAP). Information about PGAP can be found here:
            https://www.ncbi.nlm.nih.gov/genome/annotation_prok/
            
            ##Genome-Assembly-Data-START##
            Assembly Method        :: SPAdes v. 3.11.1
            Genome Representation  :: Full
            Expected Final Version :: Yes
            Genome Coverage        :: Not Applicable
            Sequencing Technology  :: Illumina MiSeq
            ##Genome-Assembly-Data-END##
            
            ##Genome-Annotation-Data-START##
            Annotation Provider               :: NCBI
            Annotation Date                   :: 05/04/2023 04:57:10
            Annotation Pipeline               :: NCBI Prokaryotic Genome
                                                 Annotation Pipeline (PGAP)
            Annotation Method                 :: Best-placed reference protein
                                                 set; GeneMarkS-2+
            Annotation Software revision      :: 6.5
            Features Annotated                :: Gene; CDS; rRNA; tRNA; ncRNA
            Genes (total)                     :: 2,776
            CDSs (total)                      :: 2,750
            Genes (coding)                    :: 2,714
            CDSs (with protein)               :: 2,714
            Genes (RNA)                       :: 26
            tRNAs                             :: 24
            ncRNAs                            :: 2
            Pseudo Genes (total)              :: 36
            CDSs (without protein)            :: 36
            Pseudo Genes (ambiguous residues) :: 0 of 36
            Pseudo Genes (frameshifted)       :: 2 of 36
            Pseudo Genes (incomplete)         :: 32 of 36
            Pseudo Genes (internal stop)      :: 2 of 36
            ##Genome-Annotation-Data-END##
FEATURES             Location/Qualifiers
     source          1..1961
                     /organism="Micropruina sp."
                     /mol_type="genomic DNA"
                     /submitter_seqid="NODE_23640_length_1961_cov_4.961175"
                     /isolate="AMR_MDS_2195"
                     /isolation_source="Activated sludge of municipal
                     wastewater treatment plants"
                     /db_xref="taxon:2737536"
                     /environmental_sample
                     /geo_loc_name="Canada"
                     /lat_lon="49.25 N 123.1 W"
                     /collection_date="2015-06-16"
                     /metagenome_source="wastewater metagenome"
                     /note="metagenomic"
     gene            complement(236..628)
                     /locus_tag="PLF56_13630"
     CDS             complement(236..628)
                     /locus_tag="PLF56_13630"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_014845239.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="type II toxin-antitoxin system VapC family
                     toxin"
                     /protein_id="HOQ54639.1"
                     /translation="MIVVDASAMVEALVGRDAEDDLLAALAGDIDAPHLLDVEVLSVL
                     RGLVLGGKLDPVDAVRSRRDYFAFTITRHETEPLAERIWQLRHQFTAYAASYLALAEA
                     LKVPLITCDAKLATRGHSAEIHLVPRTH"
     gene            complement(760..852)
                     /locus_tag="PLF56_13635"
                     /pseudo
     CDS             complement(760..852)
                     /locus_tag="PLF56_13635"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_002522572.1"
                     /note="incomplete; partial in the middle of a contig;
                     missing C-terminus; Derived by automated computational
                     analysis using gene prediction method: Protein Homology."
                     /pseudo
                     /codon_start=1
                     /transl_table=11
                     /product="antitoxin"
     gene            975..1490
                     /locus_tag="PLF56_13640"
     CDS             975..1490
                     /locus_tag="PLF56_13640"
                     /inference="COORDINATES: protein motif:HMM:NF024698.2"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_function: GO:0008080 - N-acetyltransferase activity
                     [Evidence IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="GNAT family N-acetyltransferase"
                     /protein_id="HOQ54640.1"
                     /translation="MLSLHEPTRDEFGVRQTWLADPGMMSYNAGWDITYPGYDRVSGC
                     IDWPESDWTAFEARLALPAARQGYYYVLDTESGQFIGHVHYLVESNGTAEIGFNVIPS
                     RRSQGLGAPFLRLLLNRVWADTAAEVIVQEFEDERVAAVKVHQKCGFTPERKTSTSHG
                     RPTRTRRLARA"
     gene            complement(1550..1867)
                     /locus_tag="PLF56_13645"
     CDS             complement(1550..1867)
                     /locus_tag="PLF56_13645"
                     /inference="COORDINATES: ab initio
                     prediction:GeneMarkS-2+"
                     /note="Derived by automated computational analysis using
                     gene prediction method: GeneMarkS-2+."
                     /codon_start=1
                     /transl_table=11
                     /product="hypothetical protein"
                     /protein_id="HOQ54641.1"
                     /translation="MGAWLKRLDEATRARYDEVVDIYGWLGAFAATLDPERDEDLRTD
                     LFYVDSMVAGWIAPGQFEKAQVPGPVLEAIAAAESNGASRSTPTRDDLRRRLERLREL
                     ASS"
BASE COUNT          343 a          667 c          624 g          327 t
ORIGIN      
        1 tcggagtcga tgaccgcagc cactcgctgc ccacgccgag tcagatagac cggatcgtga
       61 ccgactctgg cggagtccac gacatcagcc agacgcgcac gagcatcgct cacagtcatc
      121 tcactcacaa cactgagcat actcgctgga accgctcaaa cgtacatctt ggcgcgctac
      181 tgcgtcgaca gatcgtccga cgtcccgcag gatcgaccga gccagttgcg gcagatcagt
      241 gcgttcgcgg aacgaggtgg atctcggcgc tgtggccgcg ggtcgcgagt tttgcatcgc
      301 atgtgatcaa gggcaccttg agtgcttcgg cgagggcgag gtagctggcg gcgtaggcgg
      361 tgaactggtg acgcagttgc cagattcgct cggccagcgg ttcggtttcg tgtctggtga
      421 tggtgaacgc gaagtagtcg cgccgggagc gcaccgcgtc gaccggatcg agcttgccgc
      481 ccaggacgag tccacgcagc accgacaaca cctcgacatc caacaggtgc ggtgcatcga
      541 tgtcgccggc cagcgcggca agcaggtcgt cctcggcgtc gcgccccacg agcgcctcga
      601 ccatggccga ggcgtcgacg acgatcaccg acggccggcc tcgaccgcgg ccacgatctc
      661 ttcggtgctc ggccccgttg aacggtccct gtcacgcagt cgcgctgcga cctcctcatt
      721 cgtcggtcgg gcggcaatcc tggccaactc tgcccccacg taggccgaca acgacttgcc
      781 ctgcgcagcg gcacgctctt tgagagtctg cgccaccgac tcgggcacct cacggatgta
      841 cagggtcgtc atacaccgat gctagcactc tgcgagcgcg ttcggacgtg ctgcgcgcgc
      901 cggccgcggc gggcgaatgg ctgccactgc ccgcgcggcg acggtacgag gcgcggcggt
      961 ctagtgtcgt gggtgtgctg agcctccatg agccgaccag ggacgagttc ggcgtccggc
     1021 agacgtggct ggcagatccg gggatgatgt cgtataacgc gggctgggac atcacctacc
     1081 ccggctacga ccgggtctcc ggctgcatcg actggcccga gagcgactgg acggctttcg
     1141 aggcccgact cgcgctcccg gccgcccgtc agggctacta ctacgtcctc gacaccgagt
     1201 ccggccagtt catcgggcac gtccactacc tcgtggagtc gaatgggacc gccgagatcg
     1261 ggttcaacgt catccccagt cgacgcagcc aagggctcgg ggcaccgttc ctgcggctgc
     1321 tgctcaaccg ggtctgggcg gacactgcag ccgaagtcat cgtccaggag ttcgaggacg
     1381 aacgagttgc cgccgtcaag gtccaccaga agtgcggctt cacccctgag cgcaaaacct
     1441 ccacaagcca tggccggcca acacgcaccc ggcgcctcgc ccgtgcatag cgggccagta
     1501 accaacaccc gccatcgcgc aaccgccccg tgcgcggcag atgtgcgcgt taggaactcg
     1561 ccagttcgcg cagcctctca agacggcgcc ggagatcatc ccgggtcggc gttgagcgcg
     1621 aggcaccatt cgactccgct gccgcaatgg cctcgaggac aggaccgggc acctgtgcct
     1681 tctcgaactg gcccggcgcg atccagccgg cgaccatgct gtcgacatag aagaggtcag
     1741 tccgaaggtc ctcatcacgc tctggatcga gcgtcgccgc gaacgcgccc aaccatccgt
     1801 agatgtcgac tacttcgtcg tagcgcgcac gagtcgcctc gtccaaccgc ttcagccagg
     1861 ctcccatgct ggggattctg acaccttctg gagcattgcg aatgcagcag ttcagggcgt
     1921 ttggttaccg gcggttgtag atttcgcgtc ggtgcccgac g
//
LOCUS       DAOSUG010000529         1959 bp    DNA     linear   ENV 10-JUN-2024
DEFINITION  MAG TPA_asm: Micropruina sp. isolate AMR_MDS_2195
            NODE_23676_length_1959_cov_3.925420, whole genome shotgun sequence.
ACCESSION   DAOSUG010000529 DAOSUG010000000
VERSION     DAOSUG010000529.1
DBLINK      BioProject: PRJNA925477
            BioSample: SAMN32864262
            Sequence Read Archive: SRR4236673
KEYWORDS    WGS; ENV; Metagenome Assembled Genome; MAG; Third Party Data; TPA;
            TPA:assembly.
SOURCE      Micropruina sp. (wastewater metagenome)
  ORGANISM  Micropruina sp.
            Bacteria; Bacillati; Actinomycetota; Actinomycetes;
            Propionibacteriales; Nocardioidaceae; Micropruina.
REFERENCE   1  (bases 1 to 1959)
  AUTHORS   Abdulkadir,N., Saraiva,J.P., Zhang,J., Stolte,S., Gillor,O.,
            Harms,H. and Rocha,U.
  TITLE     Genome-centric analyses of 165 metagenomes show that mobile genetic
            elements are crucial for the transmission of antimicrobial
            resistance genes to pathogens in activated sludge and wastewater
  JOURNAL   Microbiol Spectr 12 (3), e0291823 (2024)
   PUBMED   38289113
REFERENCE   2  (bases 1 to 1959)
  AUTHORS   Abdulkadir,N.
  TITLE     Direct Submission
  JOURNAL   Submitted (24-MAR-2023) Environmental Microbiology, Helmholtz
            Center for Environmental Research -UFZ, Permoserstr 15, Leipzig
            04318, Germany
COMMENT     The annotation was added by the NCBI Prokaryotic Genome Annotation
            Pipeline (PGAP). Information about PGAP can be found here:
            https://www.ncbi.nlm.nih.gov/genome/annotation_prok/
            
            ##Genome-Assembly-Data-START##
            Assembly Method        :: SPAdes v. 3.11.1
            Genome Representation  :: Full
            Expected Final Version :: Yes
            Genome Coverage        :: Not Applicable
            Sequencing Technology  :: Illumina MiSeq
            ##Genome-Assembly-Data-END##
            
            ##Genome-Annotation-Data-START##
            Annotation Provider               :: NCBI
            Annotation Date                   :: 05/04/2023 04:57:10
            Annotation Pipeline               :: NCBI Prokaryotic Genome
                                                 Annotation Pipeline (PGAP)
            Annotation Method                 :: Best-placed reference protein
                                                 set; GeneMarkS-2+
            Annotation Software revision      :: 6.5
            Features Annotated                :: Gene; CDS; rRNA; tRNA; ncRNA
            Genes (total)                     :: 2,776
            CDSs (total)                      :: 2,750
            Genes (coding)                    :: 2,714
            CDSs (with protein)               :: 2,714
            Genes (RNA)                       :: 26
            tRNAs                             :: 24
            ncRNAs                            :: 2
            Pseudo Genes (total)              :: 36
            CDSs (without protein)            :: 36
            Pseudo Genes (ambiguous residues) :: 0 of 36
            Pseudo Genes (frameshifted)       :: 2 of 36
            Pseudo Genes (incomplete)         :: 32 of 36
            Pseudo Genes (internal stop)      :: 2 of 36
            ##Genome-Annotation-Data-END##
FEATURES             Location/Qualifiers
     source          1..1959
                     /organism="Micropruina sp."
                     /mol_type="genomic DNA"
                     /submitter_seqid="NODE_23676_length_1959_cov_3.925420"
                     /isolate="AMR_MDS_2195"
                     /isolation_source="Activated sludge of municipal
                     wastewater treatment plants"
                     /db_xref="taxon:2737536"
                     /environmental_sample
                     /geo_loc_name="Canada"
                     /lat_lon="49.25 N 123.1 W"
                     /collection_date="2015-06-16"
                     /metagenome_source="wastewater metagenome"
                     /note="metagenomic"
     gene            <1..293
                     /gene="rplU"
                     /locus_tag="PLF56_13650"
     CDS             <1..293
                     /gene="rplU"
                     /locus_tag="PLF56_13650"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_009639958.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_component: GO:0000311 - plastid large ribosomal subunit
                     [Evidence IEA];
                     GO_component: GO:0022625 - cytosolic large ribosomal
                     subunit [Evidence IEA];
                     GO_function: GO:0003735 - structural constituent of
                     ribosome [Evidence IEA];
                     GO_process: GO:0006412 - translation [Evidence IEA]"
                     /codon_start=3
                     /transl_table=11
                     /product="50S ribosomal protein L21"
                     /protein_id="HOQ54642.1"
                     /translation="SGGRQHKVAVGDVVDIDRLDAEVGSSVELTPLLVVDGESVTSEA
                     SKLAAIKVSAEVLAEVKGKKIHILKYKNKTGYKKRQGHRQKYTQVKVTGIEG"
     gene            305..562
                     /gene="rpmA"
                     /locus_tag="PLF56_13655"
     CDS             305..562
                     /gene="rpmA"
                     /locus_tag="PLF56_13655"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_020577387.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_component: GO:0000315 - organellar large ribosomal
                     subunit [Evidence IEA];
                     GO_component: GO:0022625 - cytosolic large ribosomal
                     subunit [Evidence IEA];
                     GO_function: GO:0003735 - structural constituent of
                     ribosome [Evidence IEA];
                     GO_process: GO:0006412 - translation [Evidence IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="50S ribosomal protein L27"
                     /protein_id="HOQ54643.1"
                     /translation="MAHKKGASSSRNGRDSNSQRLGVKRFGGQQVNAGEIIVRQRGTH
                     FHPGEGVGRGGDDTLFALRAGKVEFGTRRGRRVVNIALVAE"
     gene            complement(799..1716)
                     /locus_tag="PLF56_13660"
     CDS             complement(799..1716)
                     /locus_tag="PLF56_13660"
                     /EC_number="2.7.1.15"
                     /inference="COORDINATES: protein motif:HMM:NF012515.2"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_function: GO:0005524 - ATP binding [Evidence IEA];
                     GO_function: GO:0019200 - carbohydrate kinase activity
                     [Evidence IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="ribokinase"
                     /protein_id="HOQ54644.1"
                     /translation="MSAKILLVGSVMMDLILRCERAPEPSESVLGHDYRNAPGGKGSN
                     AAVAAARAGAQVAAYASVGNDSNGDYLLERWHEAGVDTTHVLRRDGNTGFVAITLEDN
                     GQNRLIIFPGVNMETPPDELEAAFDAGCDAVLLQFEIPAATNKRAIEIANERGVITVL
                     DAGPATKNGLDGLPPVTILSPNETETKALVDIYPGDEDSCREAAKRLQQKNGCTYVVL
                     KLGERGSYIYGEDVDTFVPPFKVDTVDPTAAGDAFTGTLAKAYAETGDIVAAARYGGA
                     AGALTCTKLGAQPSVPSATEIDALMAEQG"
BASE COUNT          318 a          624 c          674 g          343 t
ORIGIN      
        1 gcagtggcgg acgccagcac aaggttgctg tcggcgatgt ggtcgacatc gatcggctcg
       61 atgccgaggt ggggtccagc gtcgagttga ccccgctgct ggtggtcgat ggcgagtccg
      121 tgacctccga ggcgagcaag cttgccgcga tcaaggtcag cgccgaggtg ctggccgagg
      181 tcaagggcaa gaagatccac attctgaagt acaagaacaa gaccgggtac aagaagcgcc
      241 aggggcaccg gcagaagtac acccaggtca aggtcaccgg aatcgaaggc tgaggtagca
      301 cgagatggca cacaagaagg gcgcatccag ctcccgtaac ggtcgcgact ccaacagcca
      361 gcggctcggc gtcaagcgct tcggcggcca gcaggtcaac gcgggcgaga tcatcgtccg
      421 gcagcgcggc acccactttc accccggtga aggcgtgggc cgtggcggcg acgacaccct
      481 gttcgccctg cgcgccggca aggtcgagtt cggcacccgg cgcggccgcc gcgtcgtcaa
      541 catcgcgctc gtcgcggagt agtcaggcct cgaacaactg cactgggcgg tcctgagtgg
      601 gaccgccctt tgtcgtgccc gattcggtcc gttggaacgg tgaacacgtc gctggtcgag
      661 cttgtcggga ccgcgttgcg tgagggaacg gtgagcttcc gctgatgaag cttgtcgaga
      721 ccctgacgac aggatttcga caagctcaac tcgacggatc gcgcattgat cagccggtgc
      781 ccggacgagg ccgcgcgctc agccctgctc ggccatcagc gcgtcgatct cggtcgcgga
      841 gggaaccgac ggctgggcgc ccagcttggt gcaggtcagc gcgccggcgg ccccgccgta
      901 gcgggcagcg gcaacgatgt caccggtttc ggcgtatgcc ttggccagcg tgccggtgaa
      961 ggcgtcgcct gccgcggtcg gatcgaccgt gtcgaccttg aagggcggga cgaaggtgtc
     1021 gacgtcctcg ccgtagatgt acgaaccgcg ctcacccagc ttcagcacca cataggtgca
     1081 gccgtttttc tgctgcagtc gctttgcggc ctcgcggcag ctgtcctcgt caccggggta
     1141 gatgtccacg agagccttgg tctcggtctc attgggcgac aggatggtga ccggaggtag
     1201 cccgtccagg ccgttcttgg tggccggacc ggcgtccagc accgtgatca cgccacgctc
     1261 gttggcgatc tcgatggccc gcttgttggt ggcggctgga atctcgaact gcagtagaac
     1321 ggcgtcgcag ccggcgtcga acgcggcctc cagttcgtcc ggcggggtct ccatgttgac
     1381 gccgggaaag atgatcagcc ggttctggcc gttgtcctcg agggtgatgg ccacgaagcc
     1441 ggtgttgccg tcgcggcgca gcacgtgggt ggtgtcgacc cctgcctcgt gccagcgctc
     1501 cagcaggtag tcgccgttgg agtcgttgcc gacggacgcg tacgcggcca cctgggcacc
     1561 ggcccgggcc gcggcgacgg ccgcattgct gcctttgccg ccgggggcgt tgcggtagtc
     1621 gtggcccagc acgctctcgc tcggctcggg ggcccgttca cagcgcagga tcaggtccat
     1681 catcacgctg ccgacgagca ggatctttgc tgacatgtgt gcactccttt tgtgacacgt
     1741 gcggccgacc catcggacgc cgatgtccgg tgtgtgtgcc gactgccaga tcagcttgtc
     1801 atcaccgagt cacaggcgcg gtcaaacgcg caaaccggag cgagccctgg ggtggatcac
     1861 acgctcgagc cacgtgtcga tcagcgtttc ctggacgtca tgaccccgtc atccggctgt
     1921 gaccccgtca tccggctgtg accccgtcat cccggctgt
//
LOCUS       DAOSUG010000530         1937 bp    DNA     linear   ENV 10-JUN-2024
DEFINITION  MAG TPA_asm: Micropruina sp. isolate AMR_MDS_2195
            NODE_24038_length_1937_cov_4.508502, whole genome shotgun sequence.
ACCESSION   DAOSUG010000530 DAOSUG010000000
VERSION     DAOSUG010000530.1
DBLINK      BioProject: PRJNA925477
            BioSample: SAMN32864262
            Sequence Read Archive: SRR4236673
KEYWORDS    WGS; ENV; Metagenome Assembled Genome; MAG; Third Party Data; TPA;
            TPA:assembly.
SOURCE      Micropruina sp. (wastewater metagenome)
  ORGANISM  Micropruina sp.
            Bacteria; Bacillati; Actinomycetota; Actinomycetes;
            Propionibacteriales; Nocardioidaceae; Micropruina.
REFERENCE   1  (bases 1 to 1937)
  AUTHORS   Abdulkadir,N., Saraiva,J.P., Zhang,J., Stolte,S., Gillor,O.,
            Harms,H. and Rocha,U.
  TITLE     Genome-centric analyses of 165 metagenomes show that mobile genetic
            elements are crucial for the transmission of antimicrobial
            resistance genes to pathogens in activated sludge and wastewater
  JOURNAL   Microbiol Spectr 12 (3), e0291823 (2024)
   PUBMED   38289113
REFERENCE   2  (bases 1 to 1937)
  AUTHORS   Abdulkadir,N.
  TITLE     Direct Submission
  JOURNAL   Submitted (24-MAR-2023) Environmental Microbiology, Helmholtz
            Center for Environmental Research -UFZ, Permoserstr 15, Leipzig
            04318, Germany
COMMENT     The annotation was added by the NCBI Prokaryotic Genome Annotation
            Pipeline (PGAP). Information about PGAP can be found here:
            https://www.ncbi.nlm.nih.gov/genome/annotation_prok/
            
            ##Genome-Assembly-Data-START##
            Assembly Method        :: SPAdes v. 3.11.1
            Genome Representation  :: Full
            Expected Final Version :: Yes
            Genome Coverage        :: Not Applicable
            Sequencing Technology  :: Illumina MiSeq
            ##Genome-Assembly-Data-END##
            
            ##Genome-Annotation-Data-START##
            Annotation Provider               :: NCBI
            Annotation Date                   :: 05/04/2023 04:57:10
            Annotation Pipeline               :: NCBI Prokaryotic Genome
                                                 Annotation Pipeline (PGAP)
            Annotation Method                 :: Best-placed reference protein
                                                 set; GeneMarkS-2+
            Annotation Software revision      :: 6.5
            Features Annotated                :: Gene; CDS; rRNA; tRNA; ncRNA
            Genes (total)                     :: 2,776
            CDSs (total)                      :: 2,750
            Genes (coding)                    :: 2,714
            CDSs (with protein)               :: 2,714
            Genes (RNA)                       :: 26
            tRNAs                             :: 24
            ncRNAs                            :: 2
            Pseudo Genes (total)              :: 36
            CDSs (without protein)            :: 36
            Pseudo Genes (ambiguous residues) :: 0 of 36
            Pseudo Genes (frameshifted)       :: 2 of 36
            Pseudo Genes (incomplete)         :: 32 of 36
            Pseudo Genes (internal stop)      :: 2 of 36
            ##Genome-Annotation-Data-END##
FEATURES             Location/Qualifiers
     source          1..1937
                     /organism="Micropruina sp."
                     /mol_type="genomic DNA"
                     /submitter_seqid="NODE_24038_length_1937_cov_4.508502"
                     /isolate="AMR_MDS_2195"
                     /isolation_source="Activated sludge of municipal
                     wastewater treatment plants"
                     /db_xref="taxon:2737536"
                     /environmental_sample
                     /geo_loc_name="Canada"
                     /lat_lon="49.25 N 123.1 W"
                     /collection_date="2015-06-16"
                     /metagenome_source="wastewater metagenome"
                     /note="metagenomic"
     gene            complement(69..1487)
                     /gene="dacB"
                     /locus_tag="PLF56_13665"
     CDS             complement(69..1487)
                     /gene="dacB"
                     /locus_tag="PLF56_13665"
                     /EC_number="3.4.16.4"
                     /inference="COORDINATES: protein motif:HMM:NF014200.2"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_function: GO:0004175 - endopeptidase activity [Evidence
                     IEA];
                     GO_function: GO:0009002 - serine-type D-Ala-D-Ala
                     carboxypeptidase activity [Evidence IEA];
                     GO_process: GO:0009252 - peptidoglycan biosynthetic
                     process [Evidence IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="D-alanyl-D-alanine
                     carboxypeptidase/D-alanyl-D-alanine-endopeptidase"
                     /protein_id="HOQ54645.1"
                     /translation="MPTPAVSRRLKSRRTRTRAVARVARNLVLVLLSIVVVAAGVLLA
                     VPQWRTAVLPSGSITYTASAPPSWRAPETAPAARLPLDDLEPAAAPSAKALAARIPAL
                     PKGAAATVGLVLSDPATGKVLVKRSDRALIPASTMKLLTCLVALETLGNDRTFTTSVV
                     SPRKGVVVLRGGGDPLLSDARATGRASLQQLAATTASELKKQGVTKVALGYDASLFSG
                     PSWHPHWTDNYRYSVSPITALMVNSGFRADTGEAEKNPAQAAVKAFAKRLTKAGITVT
                     GTGPRSASAGDTVLASMQSAPVEQLIEHTLRYSDNVAAETLARHIALADGDVGSFAEA
                     EAAIRASLKTLGVWSAGMVIDDNSGLSRHNRVSPSALAGTMRLVLGEPRYRSLLLGLP
                     VGGVSGTLYDRYDDAKERAGRGVVRAKTGSLRDVNALAGYLITSDGAPLVFAIVANGV
                     TRPLEVRNWMDRTTAAWASCGC"
     gene            1561..>1937
                     /locus_tag="PLF56_13670"
     CDS             1561..>1937
                     /locus_tag="PLF56_13670"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_011183641.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="inorganic diphosphatase"
                     /protein_id="HOQ54646.1"
                     /translation="MPVVDYTRPKIQPSLHFDVTIEIPKGTKNKYEMDHHTGRIRLDR
                     TLFTSTQYPNDYGFIEGTLGQDGDPLDAMVLVPEATFPGALIECRAIGMFRMTDEMGG
                     DDKVLCIPVADQRQDYLTELKDLP"
BASE COUNT          310 a          681 c          657 g          289 t
ORIGIN      
        1 cggcgacggg cggcacacca ggtcggttgc ccgcccggaa ccgtccccga accgatgacg
       61 ccactcactc agcagccgca ggacgcccag gccgcggtgg tgcggtccat ccagttgcgc
      121 acctcgagcg gacgcgtcac gccgttggcc acgatcgcaa agaccagggg tgcgccgtcc
      181 gaggtgatca ggtacccggc cagggcattg acgtcgcgca ggctgccggt cttggcccgg
      241 acgacacccc gtccggcccg ttctttggcg tcgtcgtacc ggtcgtacag ggtgcccgag
      301 actccgccga ccggcaggcc gagcagcagg ctgcgatagc ggggctcgcc cagcaccagc
      361 ctcatcgttc cggccagcgc tgagggcgag acgcggttgt gacggctcag cccgctgttg
      421 tcgtcgatca ccatgccggc gctccacacg ccgagcgtct tcaggctcgc cctgatcgcg
      481 gcctccgctt cggcgaaact gcccacatct ccgtcggcca gggcgatgtg ccgggccagg
      541 gtctcggcgg cgacgttgtc gctgtagcgc agggtgtgct cgatcagctg ctcgaccgga
      601 gccgactgca tcgaggccag caccgtgtcg ccggcggacg cgctgcgcgg cccggtgccg
      661 gtgacggtga tgcccgcctt cgtcagccgc ttcgcgaagg ccttgaccgc tgcctgcgcc
      721 ggattcttct cggcctcgcc ggtgtcggca cggaagccgg agttcaccat caatgcggtg
      781 atcggcgaga cggagtagcg gtagttgtcg gtccagtgcg ggtgccacga cggtcccgag
      841 aacagggagg cgtcatagcc cagcgccacc ttggtcacgc cctgcttctt caactcggac
      901 gcggtggtgg ccgcgagttg ctgcagcgag gcccgtccgg tggcgcgggc gtcgctcagc
      961 agggggtcac cgccaccgcg cagcaccacc acgcccttgc gtggactcac cacggacgtg
     1021 gtgaacgtgc ggtcgttgcc cagcgtctcg agcgcaacca ggcaggtgag cagcttcatg
     1081 gtcgaggccg ggatcaatgc ccggtcggat cgcttcacca gcaccttgcc ggtggccgga
     1141 tcgctcagca ccagcccgac ggtggccgcg gcccccttcg gtagcgcggg tatgcgcgca
     1201 gccagcgctt tggcgctcgg cgccgcggcc ggctccaggt cgtccagcgg caggcgggcg
     1261 gccggggccg tctcgggtgc ccgccacgac ggcggcgccg aagccgtata ggtgatggag
     1321 cccgagggca gcaccgccgt gcgccactgc ggcaccgcca acagcacccc cgccgccacc
     1381 accacgatcg agagcagcac cagtacaagg ttgcgggcca cccgtgcgac cgcacgggtg
     1441 cgggtgcgcc tgctcttgag gcgcctgctc accgctggtg tgggcatggt gcctgctggg
     1501 gcgtagacga taggctcgaa aatggcatcc aagccatcat gacagccttt gaaaggggac
     1561 ctgcccgtgg tcgactacac gcgccccaag atccagccga gcctgcattt cgacgtcacg
     1621 atcgagattc ccaaaggcac gaagaacaag tacgagatgg accaccacac cgggcggatc
     1681 aggctcgacc gaaccctgtt cacctcgacc cagtacccca acgactacgg cttcatcgag
     1741 ggcaccctgg gccaggacgg cgatccgctg gacgcgatgg tgctcgtgcc ggaggccacg
     1801 ttcccgggcg cgctgatcga atgtcgcgcg atcggcatgt tccgcatgac cgacgagatg
     1861 ggcggtgacg acaaggtgct ctgcattccg gtcgccgacc aacgccagga ctacctgacc
     1921 gaactcaagg acctgcc
//
LOCUS       DAOSUG010000531         1927 bp    DNA     linear   ENV 10-JUN-2024
DEFINITION  MAG TPA_asm: Micropruina sp. isolate AMR_MDS_2195
            NODE_24232_length_1927_cov_3.794338, whole genome shotgun sequence.
ACCESSION   DAOSUG010000531 DAOSUG010000000
VERSION     DAOSUG010000531.1
DBLINK      BioProject: PRJNA925477
            BioSample: SAMN32864262
            Sequence Read Archive: SRR4236673
KEYWORDS    WGS; ENV; Metagenome Assembled Genome; MAG; Third Party Data; TPA;
            TPA:assembly.
SOURCE      Micropruina sp. (wastewater metagenome)
  ORGANISM  Micropruina sp.
            Bacteria; Bacillati; Actinomycetota; Actinomycetes;
            Propionibacteriales; Nocardioidaceae; Micropruina.
REFERENCE   1  (bases 1 to 1927)
  AUTHORS   Abdulkadir,N., Saraiva,J.P., Zhang,J., Stolte,S., Gillor,O.,
            Harms,H. and Rocha,U.
  TITLE     Genome-centric analyses of 165 metagenomes show that mobile genetic
            elements are crucial for the transmission of antimicrobial
            resistance genes to pathogens in activated sludge and wastewater
  JOURNAL   Microbiol Spectr 12 (3), e0291823 (2024)
   PUBMED   38289113
REFERENCE   2  (bases 1 to 1927)
  AUTHORS   Abdulkadir,N.
  TITLE     Direct Submission
  JOURNAL   Submitted (24-MAR-2023) Environmental Microbiology, Helmholtz
            Center for Environmental Research -UFZ, Permoserstr 15, Leipzig
            04318, Germany
COMMENT     The annotation was added by the NCBI Prokaryotic Genome Annotation
            Pipeline (PGAP). Information about PGAP can be found here:
            https://www.ncbi.nlm.nih.gov/genome/annotation_prok/
            
            ##Genome-Assembly-Data-START##
            Assembly Method        :: SPAdes v. 3.11.1
            Genome Representation  :: Full
            Expected Final Version :: Yes
            Genome Coverage        :: Not Applicable
            Sequencing Technology  :: Illumina MiSeq
            ##Genome-Assembly-Data-END##
            
            ##Genome-Annotation-Data-START##
            Annotation Provider               :: NCBI
            Annotation Date                   :: 05/04/2023 04:57:10
            Annotation Pipeline               :: NCBI Prokaryotic Genome
                                                 Annotation Pipeline (PGAP)
            Annotation Method                 :: Best-placed reference protein
                                                 set; GeneMarkS-2+
            Annotation Software revision      :: 6.5
            Features Annotated                :: Gene; CDS; rRNA; tRNA; ncRNA
            Genes (total)                     :: 2,776
            CDSs (total)                      :: 2,750
            Genes (coding)                    :: 2,714
            CDSs (with protein)               :: 2,714
            Genes (RNA)                       :: 26
            tRNAs                             :: 24
            ncRNAs                            :: 2
            Pseudo Genes (total)              :: 36
            CDSs (without protein)            :: 36
            Pseudo Genes (ambiguous residues) :: 0 of 36
            Pseudo Genes (frameshifted)       :: 2 of 36
            Pseudo Genes (incomplete)         :: 32 of 36
            Pseudo Genes (internal stop)      :: 2 of 36
            ##Genome-Annotation-Data-END##
FEATURES             Location/Qualifiers
     source          1..1927
                     /organism="Micropruina sp."
                     /mol_type="genomic DNA"
                     /submitter_seqid="NODE_24232_length_1927_cov_3.794338"
                     /isolate="AMR_MDS_2195"
                     /isolation_source="Activated sludge of municipal
                     wastewater treatment plants"
                     /db_xref="taxon:2737536"
                     /environmental_sample
                     /geo_loc_name="Canada"
                     /lat_lon="49.25 N 123.1 W"
                     /collection_date="2015-06-16"
                     /metagenome_source="wastewater metagenome"
                     /note="metagenomic"
     gene            complement(<1..838)
                     /locus_tag="PLF56_13675"
     CDS             complement(<1..838)
                     /locus_tag="PLF56_13675"
                     /inference="COORDINATES: protein motif:HMM:NF017745.2"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="DUF885 family protein"
                     /protein_id="HOQ54647.1"
                     /translation="MSNREPNTRQPSELDALADAYTLAYAELNPVFATQIGLAGYDHL
                     MGEAGPEFHDAADGLNRDTLRRLDAVQPRDEVDRVTAAAMRERLGLEVELHAAGEQFR
                     ALNNIASPLQEFRDVFDVMPTDTDEQWSNIASRLAGVPQAVTGYLACLREGARRGEVP
                     AALQVREGIAQAAELADPDSSFFTTFHAGRTESSALQADLQRAGLAAAESYGLLAQFL
                     RELEADAPAADASGRERYQLLSRVFLGAEVDLDETYEWGLDELARVAAEQDAVIEALA
                     GPG"
     gene            1006..>1927
                     /locus_tag="PLF56_13680"
     CDS             1006..>1927
                     /locus_tag="PLF56_13680"
                     /EC_number="2.3.-.-"
                     /inference="COORDINATES: protein motif:HMM:NF013884.2"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_function: GO:0016747 - acyltransferase activity,
                     transferring groups other than amino-acyl groups [Evidence
                     IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="acyltransferase"
                     /protein_id="HOQ54648.1"
                     /translation="MTTSVQARQVRQDIQGLRFVAVLLVVVYHVWLDRVSGGVDIFLA
                     LSAFFLTRSFARKLEQGRPLALFGYWTRTFARIVPLATLTIIAVVITSPWLMPVTVWS
                     DIAQQAIASALYYQNWWLAANDVNYYGGAPIPLQHFWSLSIQGSVFLAWPLVFAAVAG
                     VVRLRPSWRPRVLLAMAFGLIFVVSLAWSVWITATDQTFAYFDTRARLWEFAFGSLLA
                     LATMGGAAAAGPGSRLRAAVGWAGLAGILSVGVLINVRDAFPGWIALLPLTAAAAVVV
                     VGDDPGRGRVGRLLGHRWVAGLGDASYALYL"
BASE COUNT          249 a          644 c          697 g          337 t
ORIGIN      
        1 cccccggccc ggccaacgcc tcgatcaccg cgtcctgttc ggcggcgacc cgggccagct
       61 cgtccaggcc ccactcgtag gtctcgtcca gatcgacctc ggcgccgagg aacacccgcg
      121 acagcaactg atagcgctcg cgtccggacg cgtcggcagc cggtgcgtcc gcctcgagct
      181 cgcgcaggaa ctgcgccagc agtccgtacg attcggcggc ggcgagccca gcccgttgca
      241 ggtcggcctg cagtgccgac gattcggtcc ggccggcgtg gaacgtggtg aagaaggacg
      301 agtcggggtc ggccaactcg gcggcctgcg cgatgccctc gcgcacctgc agcgcagccg
      361 gcacctcgcc gcggcgagcc ccttcgcgca ggcaggccag atacccggtg acggcctgcg
      421 gaacgccggc cagccgggag gcgatgttcg accactgctc gtcggtgtcg gtgggcatca
      481 cgtcgaacac atcgcgaaac tcctgcaggg gcgaggcgat gttgttgagc gcgcggaact
      541 gctcaccggc ggcatgcaac tcgacctcga gccccagccg ctcgcgcatc gcggcggcgg
      601 tcacccggtc cacctcgtca cgcggctgga cggcgtccag cctgcgcagc gtgtcgcggt
      661 tcagcccgtc ggcggcgtcg tgaaactcgg ggccggcctc gcccatcagg tggtcgtagc
      721 ccgccagccc gatctgggtg gcgaagacgg ggttcagctc ggcgtaggcc agggtgtagg
      781 cgtcggccag ggcgtccagt tcgctgggtt gacgggtgtt cggttcacgg ttgctcaccc
      841 gggcgaggct accccgcgca ctgggtgcgg ggaatctgtg ccggtgcacc gtgagcgaga
      901 cgcgcctagt cctatcgccg aggatgacaa cgcagcgagc gggcgtacgg ggtgcgccgc
      961 agcgcggtca gggttatgcg acacgcccta gactggcgcg atcccatgac gacttccgtg
     1021 caggcacgcc aggtgcgaca ggacattcag gggctgcggt tcgtcgccgt gctgctggtg
     1081 gtggtgtatc acgtgtggct cgaccgggtg tcgggcggtg tcgatatctt tctggccctc
     1141 tcggcgttct ttctgacccg atcattcgcg cgcaagctgg agcaggggcg tccgctggcg
     1201 ctgttcggct actggacccg cacgttcgcc cgcatcgtgc ccctcgcgac gctgacgatc
     1261 atcgccgtgg tgatcacctc gccctggctg atgccggtca cggtgtggtc cgacatcgcg
     1321 caacaggcga tcgccagcgc gctgtactac cagaactggt ggcttgccgc gaacgacgtg
     1381 aactactacg gcggtgcgcc gattccgctg caacacttct ggtcgctctc gattcagggc
     1441 agcgtctttc tggcgtggcc gctggtgttc gcagccgtgg ccggggtggt gcggctgcgc
     1501 ccgagctggc gtccacgggt gctgctcgcg atggctttcg gcctgatctt cgtggtgtcg
     1561 ctcgcgtggt ccgtatggat cacggccacc gaccagacct tcgcctactt cgacacccgc
     1621 gcccgattgt gggagttcgc attcgggtca ctgctcgcac tggccacgat gggcggggca
     1681 gcggccgccg ggccaggctc gcgactgcgg gcggcggtgg gttgggctgg cctggcaggc
     1741 attctgtcgg tgggagtact gatcaacgtg cgcgacgcct ttccgggctg gatcgccctg
     1801 ctgccgttga ccgctgccgc cgccgtggtg gtggtgggcg atgatccggg acgggggcga
     1861 gtcggacggc tgctcggcca ccggtgggtc gcgggcctgg gcgatgcctc gtacgcgctc
     1921 tatctgg
//
LOCUS       DAOSUG010000532         1925 bp    DNA     linear   ENV 10-JUN-2024
DEFINITION  MAG TPA_asm: Micropruina sp. isolate AMR_MDS_2195
            NODE_24269_length_1925_cov_3.702139, whole genome shotgun sequence.
ACCESSION   DAOSUG010000532 DAOSUG010000000
VERSION     DAOSUG010000532.1
DBLINK      BioProject: PRJNA925477
            BioSample: SAMN32864262
            Sequence Read Archive: SRR4236673
KEYWORDS    WGS; ENV; Metagenome Assembled Genome; MAG; Third Party Data; TPA;
            TPA:assembly.
SOURCE      Micropruina sp. (wastewater metagenome)
  ORGANISM  Micropruina sp.
            Bacteria; Bacillati; Actinomycetota; Actinomycetes;
            Propionibacteriales; Nocardioidaceae; Micropruina.
REFERENCE   1  (bases 1 to 1925)
  AUTHORS   Abdulkadir,N., Saraiva,J.P., Zhang,J., Stolte,S., Gillor,O.,
            Harms,H. and Rocha,U.
  TITLE     Genome-centric analyses of 165 metagenomes show that mobile genetic
            elements are crucial for the transmission of antimicrobial
            resistance genes to pathogens in activated sludge and wastewater
  JOURNAL   Microbiol Spectr 12 (3), e0291823 (2024)
   PUBMED   38289113
REFERENCE   2  (bases 1 to 1925)
  AUTHORS   Abdulkadir,N.
  TITLE     Direct Submission
  JOURNAL   Submitted (24-MAR-2023) Environmental Microbiology, Helmholtz
            Center for Environmental Research -UFZ, Permoserstr 15, Leipzig
            04318, Germany
COMMENT     The annotation was added by the NCBI Prokaryotic Genome Annotation
            Pipeline (PGAP). Information about PGAP can be found here:
            https://www.ncbi.nlm.nih.gov/genome/annotation_prok/
            
            ##Genome-Assembly-Data-START##
            Assembly Method        :: SPAdes v. 3.11.1
            Genome Representation  :: Full
            Expected Final Version :: Yes
            Genome Coverage        :: Not Applicable
            Sequencing Technology  :: Illumina MiSeq
            ##Genome-Assembly-Data-END##
            
            ##Genome-Annotation-Data-START##
            Annotation Provider               :: NCBI
            Annotation Date                   :: 05/04/2023 04:57:10
            Annotation Pipeline               :: NCBI Prokaryotic Genome
                                                 Annotation Pipeline (PGAP)
            Annotation Method                 :: Best-placed reference protein
                                                 set; GeneMarkS-2+
            Annotation Software revision      :: 6.5
            Features Annotated                :: Gene; CDS; rRNA; tRNA; ncRNA
            Genes (total)                     :: 2,776
            CDSs (total)                      :: 2,750
            Genes (coding)                    :: 2,714
            CDSs (with protein)               :: 2,714
            Genes (RNA)                       :: 26
            tRNAs                             :: 24
            ncRNAs                            :: 2
            Pseudo Genes (total)              :: 36
            CDSs (without protein)            :: 36
            Pseudo Genes (ambiguous residues) :: 0 of 36
            Pseudo Genes (frameshifted)       :: 2 of 36
            Pseudo Genes (incomplete)         :: 32 of 36
            Pseudo Genes (internal stop)      :: 2 of 36
            ##Genome-Annotation-Data-END##
FEATURES             Location/Qualifiers
     source          1..1925
                     /organism="Micropruina sp."
                     /mol_type="genomic DNA"
                     /submitter_seqid="NODE_24269_length_1925_cov_3.702139"
                     /isolate="AMR_MDS_2195"
                     /isolation_source="Activated sludge of municipal
                     wastewater treatment plants"
                     /db_xref="taxon:2737536"
                     /environmental_sample
                     /geo_loc_name="Canada"
                     /lat_lon="49.25 N 123.1 W"
                     /collection_date="2015-06-16"
                     /metagenome_source="wastewater metagenome"
                     /note="metagenomic"
     gene            76..249
                     /locus_tag="PLF56_13685"
     CDS             76..249
                     /locus_tag="PLF56_13685"
                     /inference="COORDINATES: ab initio
                     prediction:GeneMarkS-2+"
                     /note="Derived by automated computational analysis using
                     gene prediction method: GeneMarkS-2+."
                     /codon_start=1
                     /transl_table=11
                     /product="hypothetical protein"
                     /protein_id="HOQ54649.1"
                     /translation="MTTHFYTASSLDGFIATPEHSLEWLFKQDFDFEGPMAYPEFVKG
                     IGALFMGASTYEP"
     gene            complement(386..1759)
                     /locus_tag="PLF56_13690"
     CDS             complement(386..1759)
                     /locus_tag="PLF56_13690"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_020580013.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_function: GO:0004803 - transposase activity [Evidence
                     IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="IS1380 family transposase"
                     /protein_id="HOQ54650.1"
                     /translation="MKPSHTIRPVFDDPNLVSAAGLVPALRLAGSAGFYDLLEGLTVA
                     SPNASAKTAAVVGGMLAGADSIDDLDLLRHGGMDRLFAGVRAPSTLGTFLRSFTHGHV
                     QQLDKIGGGLLSGLAARVPGLLAGGGAEGIAFLDVDDTVREVHGYAKQGAAFGYTGAR
                     GLNVQLATISTPTAAPVIARARLRKGNTASATGCGRLLAQAITTARAAGVTGRILARA
                     DSAYYGWAFVGTALRHQAWFSVTARMTPSVTAAIASIDPGEWTAIAYPNAVFDEVEQR
                     WVSDAEVAEVPFTAFTGRRKAEHVSCRLVVRRVKRVQPLASDGSEQGELFATYRHHAF
                     ITNSTLSTIEADQRHRDHALVEQVIAELKDGPLAHLPSGKYAANAAWVACSVLAFNIA
                     RATAVAAAMRTARWATVRTRILNIPARIATTGRRLVLHLPTHWPWAPGWESLWSAATG
                     PPTAVTT"
BASE COUNT          315 a          620 c          671 g          319 t
ORIGIN      
        1 taacttgcgg cttgcgctca atgatgcaga actctgaccc tgatttccag aacatcgcaa
       61 gaaaggtgcg ctcccatgac cacccacttc tatactgctt caagcctcga cgggttcatc
      121 gccacccccg agcattccct tgagtggctg ttcaagcagg acttcgattt cgaaggcccc
      181 atggcatacc cagagttcgt gaaaggaatt ggcgctctct tcatgggtgc ttccacgtat
      241 gagccctgat ccaccggtga tgattcgtca tcggtgattc cggtggatcg cgacgaggct
      301 cgcagtgtgg gcgtgggtga gctgccggcc ggtgccggct cttccacttc gaggtcctcg
      361 ggttgtgccg tgtgcggcag ggtggtcagg tcgtgacagc ggtgggcggg ccggtcgcgg
      421 ccgaccacag tgactcccag ccgggtgccc agggccagtg cgtgggcagg tgcagcacga
      481 gccgtcggcc ggtggtcgcg atccgggcgg ggatgttcag gatgcgcgtg cgcacagtgg
      541 cccaccgggc ggtgcgcatc gctgcggcga cagcggtggc acgggcgatg ttgaacgcga
      601 gcacgctgca cgccacccag gccgcgttcg ccgcgtactt gcccgatggc aggtgggcca
      661 gggggccgtc cttgagttcg gcgatgacct gctcgaccag ggcgtggtcg cggtgacgct
      721 gatcggcctc gatcgtggac agggtgctgt tggtgatgaa ggcgtggtgg cggtaggtgg
      781 cgaacagctc gccctgttcg ctgccgtcag atgccagtgg ctggactcgc ttgacgcggc
      841 gcacgaccag acgacacgac acgtgctcgg ccttgcgtcg gccggtgaac gcagtgaacg
      901 gcacttcagc gacctcggcg tcgctgaccc agcgttgctc gacctcgtcg aagaccgcgt
      961 tcgggtaggc gatcgcggtc cactcgcccg ggtcgatgct ggcgatcgcc gcggtcaccg
     1021 acggggtcat ccgggcggtg accgagaacc aggcttggtg gcgcagcgcg gtcccgacga
     1081 acgcccagcc gtagtaggcc gagtccgcgc gagccaagat ccgcccggtc acgccagcag
     1141 cccgggcggt ggtgatcgcc tgtgccagca accggccgca acccgtggcg gaggcagtgt
     1201 tgcccttacg gagccgggca cgcgcgatca ccggcgcagc ggtcggagtc gagatcgtgg
     1261 cgagctggac gttcagcccc ctggcgccgg tgtagccgaa cgcggccccc tgcttggcat
     1321 agccgtggac ctcacgcacg gtgtcatcga catcgaggaa cgcgatcccc tccgcgccgc
     1381 caccggccag cagtccaggc acccgcgcgg caagcccgga cagcagtccg ccgccgatct
     1441 tgtcgagctg ctggacatgg ccgtgggtga aggaacgcag gaacgtgccc agcgtcgagg
     1501 gcgcccgaac cccggcgaac aaccgatcca tcccgccgtg gcgcagcagg tcgaggtcat
     1561 cgatggagtc cgcaccggcg agcatcccgc cgacgaccgc ggcggtcttc gccgaggcgt
     1621 tgggcgaggc caccgtcagg ccctcgagga ggtcgtagaa gccggcagat ccggccagcc
     1681 gcagcgccgg taccagacct gcggccgaca ccagattcgg gtcatcgaac acaggtcgga
     1741 tcgtgtgaga aggtttcact tacgaggtgc ctctcgtgag cggttgggtg acgacgtcgc
     1801 aatcgacatc ttccctgctc aggagggcac ctcgttctac gccacgctca cagaccccac
     1861 cgcagccggt ggatcagggt tgagagatcc atatctcgcg gactgggcgg tagtggaata
     1921 cggct
//
LOCUS       DAOSUG010000533         1919 bp    DNA     linear   ENV 10-JUN-2024
DEFINITION  MAG TPA_asm: Micropruina sp. isolate AMR_MDS_2195
            NODE_24371_length_1919_cov_4.054721, whole genome shotgun sequence.
ACCESSION   DAOSUG010000533 DAOSUG010000000
VERSION     DAOSUG010000533.1
DBLINK      BioProject: PRJNA925477
            BioSample: SAMN32864262
            Sequence Read Archive: SRR4236673
KEYWORDS    WGS; ENV; Metagenome Assembled Genome; MAG; Third Party Data; TPA;
            TPA:assembly.
SOURCE      Micropruina sp. (wastewater metagenome)
  ORGANISM  Micropruina sp.
            Bacteria; Bacillati; Actinomycetota; Actinomycetes;
            Propionibacteriales; Nocardioidaceae; Micropruina.
REFERENCE   1  (bases 1 to 1919)
  AUTHORS   Abdulkadir,N., Saraiva,J.P., Zhang,J., Stolte,S., Gillor,O.,
            Harms,H. and Rocha,U.
  TITLE     Genome-centric analyses of 165 metagenomes show that mobile genetic
            elements are crucial for the transmission of antimicrobial
            resistance genes to pathogens in activated sludge and wastewater
  JOURNAL   Microbiol Spectr 12 (3), e0291823 (2024)
   PUBMED   38289113
REFERENCE   2  (bases 1 to 1919)
  AUTHORS   Abdulkadir,N.
  TITLE     Direct Submission
  JOURNAL   Submitted (24-MAR-2023) Environmental Microbiology, Helmholtz
            Center for Environmental Research -UFZ, Permoserstr 15, Leipzig
            04318, Germany
COMMENT     The annotation was added by the NCBI Prokaryotic Genome Annotation
            Pipeline (PGAP). Information about PGAP can be found here:
            https://www.ncbi.nlm.nih.gov/genome/annotation_prok/
            
            ##Genome-Assembly-Data-START##
            Assembly Method        :: SPAdes v. 3.11.1
            Genome Representation  :: Full
            Expected Final Version :: Yes
            Genome Coverage        :: Not Applicable
            Sequencing Technology  :: Illumina MiSeq
            ##Genome-Assembly-Data-END##
            
            ##Genome-Annotation-Data-START##
            Annotation Provider               :: NCBI
            Annotation Date                   :: 05/04/2023 04:57:10
            Annotation Pipeline               :: NCBI Prokaryotic Genome
                                                 Annotation Pipeline (PGAP)
            Annotation Method                 :: Best-placed reference protein
                                                 set; GeneMarkS-2+
            Annotation Software revision      :: 6.5
            Features Annotated                :: Gene; CDS; rRNA; tRNA; ncRNA
            Genes (total)                     :: 2,776
            CDSs (total)                      :: 2,750
            Genes (coding)                    :: 2,714
            CDSs (with protein)               :: 2,714
            Genes (RNA)                       :: 26
            tRNAs                             :: 24
            ncRNAs                            :: 2
            Pseudo Genes (total)              :: 36
            CDSs (without protein)            :: 36
            Pseudo Genes (ambiguous residues) :: 0 of 36
            Pseudo Genes (frameshifted)       :: 2 of 36
            Pseudo Genes (incomplete)         :: 32 of 36
            Pseudo Genes (internal stop)      :: 2 of 36
            ##Genome-Annotation-Data-END##
FEATURES             Location/Qualifiers
     source          1..1919
                     /organism="Micropruina sp."
                     /mol_type="genomic DNA"
                     /submitter_seqid="NODE_24371_length_1919_cov_4.054721"
                     /isolate="AMR_MDS_2195"
                     /isolation_source="Activated sludge of municipal
                     wastewater treatment plants"
                     /db_xref="taxon:2737536"
                     /environmental_sample
                     /geo_loc_name="Canada"
                     /lat_lon="49.25 N 123.1 W"
                     /collection_date="2015-06-16"
                     /metagenome_source="wastewater metagenome"
                     /note="metagenomic"
     gene            276..1319
                     /locus_tag="PLF56_13695"
     CDS             276..1319
                     /locus_tag="PLF56_13695"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_015071818.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="alcohol dehydrogenase catalytic
                     domain-containing protein"
                     /protein_id="HOQ54651.1"
                     /translation="MRGFTYRGPGVPAELTDLPDPIAGPGQVLTRVGANTVCGTDLRI
                     LRGEKSKGVELGVVLGHEVAGYVEAVGEGVNGFEVGDLVSMPPSVCCGVCGPCRRGTE
                     HLCDDVHLVGYDINGGLADYWLIPREAIARGQLVKASGHLEPAQLALAEPISCCLSGL
                     DNYRVEVDDVVVIVGAGPIGLIHLQLARIAGARTVVVSDPSEVRRTTAAALGASVTVD
                     PTTQDLPAIVRELSGGEGVDVAVLCIGVPQLVNDCLNLVRKRGRVSIFAGLAGEGWST
                     IAANLIHYREITVVGASNSGRESFARAVRLIESGAIDTASLITHTFPLSRATEAIEFV
                     ASGEGIKVAVVPD"
     gene            1549..>1919
                     /locus_tag="PLF56_13700"
     CDS             1549..>1919
                     /locus_tag="PLF56_13700"
                     /inference="COORDINATES: protein motif:HMM:NF012589.2"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_function: GO:0016773 - phosphotransferase activity,
                     alcohol group as acceptor [Evidence IEA];
                     GO_process: GO:0005975 - carbohydrate metabolic process
                     [Evidence IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="FGGY family carbohydrate kinase"
                     /protein_id="HOQ54652.1"
                     /translation="MAKDKRLVIGIDFGTGSSKGVLARLNGKVIATAEKAHRTSMPKP
                     GHVEHDADEVWWADFLDITHDLLSKADGPVVGITTSGIGPCSLFCDAEGKPLRPAILY
                     GVDTRAYKEVAELTDKIGVDAL"
BASE COUNT          331 a          667 c          635 g          286 t
ORIGIN      
        1 ggctcaaacc cgtctgcccg caagggcata ggggttcgac tcccctcctc ggcacgcatc
       61 tactcgcgca ctcagcgcgc atcccgccag tcaacgcgcc cgactccgct cgcccgagca
      121 gcgcgccgac gacccgctcg acccgacgcc actccaagac aaaggccaaa ggccccgccc
      181 acggcgagcg gtttcggcca tagggtggag agcgttcacc gcgccgggtc gcaacgacgc
      241 atgacccggt gcagctcgtg ctgaaggagc agaccatgcg cggattcacc tatcgcggcc
      301 cgggcgtacc ggccgaactg accgacctgc ccgacccgat cgccggcccg ggccaggtgc
      361 tcaccagggt gggcgccaac accgtctgcg gtaccgatct acgcattctg cgcggcgaga
      421 agtcgaaggg cgtcgaactg ggcgtggtgc tgggccacga ggttgcgggc tatgtcgaag
      481 cggtcggcga aggcgtcaac ggcttcgagg tgggcgacct ggtcagtatg ccgccgtcgg
      541 tctgctgcgg cgtgtgtggt ccgtgccggc ggggtaccga gcacctgtgc gacgacgtcc
      601 acctggtcgg ctacgacatc aacgggggcc tggcggacta ctggctgatc ccccgagaag
      661 cgatcgcacg cggccaactg gtcaaggcgt cgggtcacct cgagcccgcc cagctggcgt
      721 tggccgagcc gatctcgtgc tgcctcagcg gcctggacaa ctaccgggtc gaggtggacg
      781 acgtcgtggt gatcgtcggc gcagggccga tcggcctgat ccacctgcag cttgcccgca
      841 tcgccggcgc acgcacggtc gtggtgtctg acccgtccga ggtgcggcgc accacggctg
      901 ccgcgctcgg agcgagcgtc accgtcgacc cgaccactca ggacctgccc gcgatcgtcc
      961 gcgaactcag cggcggggaa ggcgtggacg tggcggtgct ctgcatcggc gtgccgcaac
     1021 tcgtcaacga ctgcctgaac ctggtgcgca agcgcggccg ggtgagcatc ttcgccgggc
     1081 tggccggcga gggttggtcc accatcgccg ccaacctgat ccactaccgc gagatcaccg
     1141 tggtgggtgc gtccaactcg ggacgcgaga gcttcgcacg cgccgtccgg ctgatcgaga
     1201 gcggcgccat cgacaccgca tcgctgatca cccacacctt ccccctgtcg cgggccaccg
     1261 aggcgatcga gttcgtcgct tccggcgagg gcatcaaggt tgccgtggtg cccgactgaa
     1321 gctgccggca ccgactggcg ggcaccacca ggcccttcgg tacgaacgga ctccgtgcga
     1381 tccgagcttt gtctcacgaa aaatgcgaca aaccacaaca agttcacact tatcccgccg
     1441 atcccctccc gttgggcgct ccgatcgcat accttttggt tgtgggaagg gggcaccgtc
     1501 gccgtcctcc ccgattgagc aacgatgctc gcggaaagga gcaccagcat ggcgaaggac
     1561 aagaggttgg tcatcgggat cgacttcggc accggaagca gcaagggcgt actggcccgg
     1621 ctgaacggca aggtcatcgc aacggcggag aaggcgcacc gcacctcgat gcccaagccc
     1681 gggcacgtcg aacacgatgc ggacgaggtc tggtgggccg acttcctgga catcactcat
     1741 gacctgctga gcaaggcgga cggcccggtg gtcggcatca ccacctcagg catcggcccc
     1801 tgcagcctgt tctgcgacgc ggagggcaag ccgctgcgtc cggccatcct gtacggcgtc
     1861 gacacgcgcg cctacaaaga ggtcgccgaa ctcaccgaca agatcggcgt ggacgccct
//
LOCUS       DAOSUG010000534         1910 bp    DNA     linear   ENV 10-JUN-2024
DEFINITION  MAG TPA_asm: Micropruina sp. isolate AMR_MDS_2195
            NODE_24535_length_1910_cov_2.941779, whole genome shotgun sequence.
ACCESSION   DAOSUG010000534 DAOSUG010000000
VERSION     DAOSUG010000534.1
DBLINK      BioProject: PRJNA925477
            BioSample: SAMN32864262
            Sequence Read Archive: SRR4236673
KEYWORDS    WGS; ENV; Metagenome Assembled Genome; MAG; Third Party Data; TPA;
            TPA:assembly.
SOURCE      Micropruina sp. (wastewater metagenome)
  ORGANISM  Micropruina sp.
            Bacteria; Bacillati; Actinomycetota; Actinomycetes;
            Propionibacteriales; Nocardioidaceae; Micropruina.
REFERENCE   1  (bases 1 to 1910)
  AUTHORS   Abdulkadir,N., Saraiva,J.P., Zhang,J., Stolte,S., Gillor,O.,
            Harms,H. and Rocha,U.
  TITLE     Genome-centric analyses of 165 metagenomes show that mobile genetic
            elements are crucial for the transmission of antimicrobial
            resistance genes to pathogens in activated sludge and wastewater
  JOURNAL   Microbiol Spectr 12 (3), e0291823 (2024)
   PUBMED   38289113
REFERENCE   2  (bases 1 to 1910)
  AUTHORS   Abdulkadir,N.
  TITLE     Direct Submission
  JOURNAL   Submitted (24-MAR-2023) Environmental Microbiology, Helmholtz
            Center for Environmental Research -UFZ, Permoserstr 15, Leipzig
            04318, Germany
COMMENT     The annotation was added by the NCBI Prokaryotic Genome Annotation
            Pipeline (PGAP). Information about PGAP can be found here:
            https://www.ncbi.nlm.nih.gov/genome/annotation_prok/
            
            ##Genome-Assembly-Data-START##
            Assembly Method        :: SPAdes v. 3.11.1
            Genome Representation  :: Full
            Expected Final Version :: Yes
            Genome Coverage        :: Not Applicable
            Sequencing Technology  :: Illumina MiSeq
            ##Genome-Assembly-Data-END##
            
            ##Genome-Annotation-Data-START##
            Annotation Provider               :: NCBI
            Annotation Date                   :: 05/04/2023 04:57:10
            Annotation Pipeline               :: NCBI Prokaryotic Genome
                                                 Annotation Pipeline (PGAP)
            Annotation Method                 :: Best-placed reference protein
                                                 set; GeneMarkS-2+
            Annotation Software revision      :: 6.5
            Features Annotated                :: Gene; CDS; rRNA; tRNA; ncRNA
            Genes (total)                     :: 2,776
            CDSs (total)                      :: 2,750
            Genes (coding)                    :: 2,714
            CDSs (with protein)               :: 2,714
            Genes (RNA)                       :: 26
            tRNAs                             :: 24
            ncRNAs                            :: 2
            Pseudo Genes (total)              :: 36
            CDSs (without protein)            :: 36
            Pseudo Genes (ambiguous residues) :: 0 of 36
            Pseudo Genes (frameshifted)       :: 2 of 36
            Pseudo Genes (incomplete)         :: 32 of 36
            Pseudo Genes (internal stop)      :: 2 of 36
            ##Genome-Annotation-Data-END##
FEATURES             Location/Qualifiers
     source          1..1910
                     /organism="Micropruina sp."
                     /mol_type="genomic DNA"
                     /submitter_seqid="NODE_24535_length_1910_cov_2.941779"
                     /isolate="AMR_MDS_2195"
                     /isolation_source="Activated sludge of municipal
                     wastewater treatment plants"
                     /db_xref="taxon:2737536"
                     /environmental_sample
                     /geo_loc_name="Canada"
                     /lat_lon="49.25 N 123.1 W"
                     /collection_date="2015-06-16"
                     /metagenome_source="wastewater metagenome"
                     /note="metagenomic"
     gene            <1..824
                     /gene="dapE"
                     /locus_tag="PLF56_13705"
     CDS             <1..824
                     /gene="dapE"
                     /locus_tag="PLF56_13705"
                     /EC_number="3.5.1.18"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_013865158.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_function: GO:0009014 - succinyl-diaminopimelate
                     desuccinylase activity [Evidence IEA];
                     GO_process: GO:0009089 - lysine biosynthetic process via
                     diaminopimelate [Evidence IEA]"
                     /codon_start=3
                     /transl_table=11
                     /product="succinyl-diaminopimelate desuccinylase"
                     /protein_id="HOQ54653.1"
                     /translation="SWVEGERIYGRGTCDMKGGIAVMLAVAVAADEPVHDLTWIFYDH
                     EEVESSANSLTRIAADRPELLAGDFAVLMEPTAARVEGGCQGTLRFTVTTTGRAAHSA
                     RAWMGHNAIHDAADVLAVLRDHECEQPEVDGLRYHEGLNAVAITGGIAGNVIPDACTV
                     TVNYRFAPHRTIEQAKQYCREVLPGHELVFTDEAAGARPGLDLPAAAQFVAAVGGQPS
                     AKFGWTDVARFSALGVPAVNYGPGDPSKAHADDEFCPVPDLISCRDALLRWLTEG"
     gene            968..1894
                     /locus_tag="PLF56_13710"
     CDS             968..1894
                     /locus_tag="PLF56_13710"
                     /inference="COORDINATES: protein motif:HMM:TIGR00350.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="LCP family protein"
                     /protein_id="HOQ54654.1"
                     /translation="MRRLRRVRAGLLAAAVAIAVVAASAVGSLWLSLAGIERDPGLMP
                     QRTAGSSAGALNLLVIGSDSHDLTGRASVLMVAHVDAERRNVHLISLPCELLVTGADG
                     GTDRLSHLFDRGGAAPVVVAVERQLGITIDHVAVTWLAGMSRLIDLLGGIPVDNPVAA
                     TTGRFAFPRGQITLSGEEALDFVRSDAAVHGAGDLDRAESQRLVLQGLVTRLLTSTAL
                     INPGVVKAVLDQLAADIVVDSGLDARRMAELFFALRTRSGAQQVGAIKLPTAERGVLP
                     SGETYVRADPDRAPALGRAIASDTLESWERNR"
BASE COUNT          258 a          675 c          680 g          297 t
ORIGIN      
        1 cgtcatgggt cgagggcgag cgcatctacg gccgtggcac ctgcgacatg aagggtggca
       61 tcgcggtgat gctggcggtg gccgtggccg cggatgaacc cgtgcacgac ctgacctgga
      121 tcttctacga ccacgaggag gtcgaatcgt cggccaactc gctgacccgc atcgcggccg
      181 accgccccga gttgctggcc ggcgacttcg ccgtactgat ggagccgacc gcggcccggg
      241 tcgaaggcgg ttgtcagggc accctgcggt tcaccgtcac caccacgggc cgggccgcgc
      301 attcggcccg ggcctggatg ggccacaacg ccatccacga cgccgccgac gtgctcgcag
      361 tgctgcgcga ccacgagtgc gagcagcccg aggtggacgg cctgcgctac cacgagggcc
      421 tcaacgccgt ggccatcaca ggcggcattg cgggcaacgt gatacccgat gcctgcacgg
      481 tcacggtcaa ctaccggttc gcgccgcatc gcacgatcga gcaggccaag cagtactgcc
      541 gggaggtctt gcccgggcac gagctggtct tcaccgacga ggccgccggc gcgcgtccgg
      601 ggctcgacct gccggccgcc gcccagttcg tcgcagccgt cggcgggcag ccgtcggcca
      661 agttcggctg gacggacgtc gcccgcttct ctgcgctcgg cgtgccggcg gtcaactacg
      721 gcccgggcga cccgtccaag gcgcacgccg atgacgagtt ctgcccggtc cccgacctga
      781 tcagctgccg tgacgcgctg ctgcgctggc tcacggaggg gtagctgagc gcctgtgtat
      841 gctgtggcga tgcggccgcc tgtcgggtga ggacggcagc cgcttcgatg cttgagccga
      901 ccggggggat cgggccatca gcgacttttt gtcctattcg cgtacgcacc cacgcggcag
      961 cgcgggcatg cggcggctgc ggcgcgtccg cgcggggctg ctcgccgccg ccgttgcgat
     1021 cgccgtggtt gctgcctccg ccgtcgggtc gctgtggctg tccctcgccg gcatcgagcg
     1081 cgaccccggg ctgatgccgc agcgcacggc cggctcgtcc gcgggcgcac tcaacctgct
     1141 ggtgatcggt tcggactcac acgacctcac gggccgggcc agcgtgctga tggtcgcgca
     1201 tgtggatgcg gagcggcgca acgttcatct gatctcgctg ccgtgcgagt tgctggtgac
     1261 gggcgccgac ggcggcacgg atcggctcag ccacctgttc gaccgcggcg gcgcggcccc
     1321 cgtggtggta gccgtcgaac gccaactcgg catcaccatc gaccatgtgg cggtgacctg
     1381 gctggccgga atgtcgcggc tgatcgacct gctcggcggc attccggtcg acaatccggt
     1441 cgccgccacc accggacgat tcgcctttcc gcgcggccag atcaccctct cgggcgagga
     1501 ggcgctcgac ttcgtgcgtt cggacgctgc cgtccacggc gccggcgatc tggatcgggc
     1561 cgagagccag cgcctggtgc tgcagggact ggtcaccagg ctgctgacca gcaccgcgtt
     1621 gatcaatccg ggggtggtca aggctgtgct cgaccaactc gccgccgaca tcgtcgtcga
     1681 cagcggcctg gacgcccgtc gcatggccga gctgttcttc gcactgcgca cccggtccgg
     1741 tgcgcagcag gtgggggcga tcaagctgcc caccgccgaa cggggcgtgc tgccgtccgg
     1801 cgaaacctac gtgagggcgg atcccgaccg ggccccggcg ctgggccgcg cgatcgcgtc
     1861 cgacaccttg gagtcctggg aacgcaaccg ctgaccggtc gtgcgcatcg
//
LOCUS       DAOSUG010000535         1881 bp    DNA     linear   ENV 10-JUN-2024
DEFINITION  MAG TPA_asm: Micropruina sp. isolate AMR_MDS_2195
            NODE_25037_length_1881_cov_3.940854, whole genome shotgun sequence.
ACCESSION   DAOSUG010000535 DAOSUG010000000
VERSION     DAOSUG010000535.1
DBLINK      BioProject: PRJNA925477
            BioSample: SAMN32864262
            Sequence Read Archive: SRR4236673
KEYWORDS    WGS; ENV; Metagenome Assembled Genome; MAG; Third Party Data; TPA;
            TPA:assembly.
SOURCE      Micropruina sp. (wastewater metagenome)
  ORGANISM  Micropruina sp.
            Bacteria; Bacillati; Actinomycetota; Actinomycetes;
            Propionibacteriales; Nocardioidaceae; Micropruina.
REFERENCE   1  (bases 1 to 1881)
  AUTHORS   Abdulkadir,N., Saraiva,J.P., Zhang,J., Stolte,S., Gillor,O.,
            Harms,H. and Rocha,U.
  TITLE     Genome-centric analyses of 165 metagenomes show that mobile genetic
            elements are crucial for the transmission of antimicrobial
            resistance genes to pathogens in activated sludge and wastewater
  JOURNAL   Microbiol Spectr 12 (3), e0291823 (2024)
   PUBMED   38289113
REFERENCE   2  (bases 1 to 1881)
  AUTHORS   Abdulkadir,N.
  TITLE     Direct Submission
  JOURNAL   Submitted (24-MAR-2023) Environmental Microbiology, Helmholtz
            Center for Environmental Research -UFZ, Permoserstr 15, Leipzig
            04318, Germany
COMMENT     The annotation was added by the NCBI Prokaryotic Genome Annotation
            Pipeline (PGAP). Information about PGAP can be found here:
            https://www.ncbi.nlm.nih.gov/genome/annotation_prok/
            
            ##Genome-Assembly-Data-START##
            Assembly Method        :: SPAdes v. 3.11.1
            Genome Representation  :: Full
            Expected Final Version :: Yes
            Genome Coverage        :: Not Applicable
            Sequencing Technology  :: Illumina MiSeq
            ##Genome-Assembly-Data-END##
            
            ##Genome-Annotation-Data-START##
            Annotation Provider               :: NCBI
            Annotation Date                   :: 05/04/2023 04:57:10
            Annotation Pipeline               :: NCBI Prokaryotic Genome
                                                 Annotation Pipeline (PGAP)
            Annotation Method                 :: Best-placed reference protein
                                                 set; GeneMarkS-2+
            Annotation Software revision      :: 6.5
            Features Annotated                :: Gene; CDS; rRNA; tRNA; ncRNA
            Genes (total)                     :: 2,776
            CDSs (total)                      :: 2,750
            Genes (coding)                    :: 2,714
            CDSs (with protein)               :: 2,714
            Genes (RNA)                       :: 26
            tRNAs                             :: 24
            ncRNAs                            :: 2
            Pseudo Genes (total)              :: 36
            CDSs (without protein)            :: 36
            Pseudo Genes (ambiguous residues) :: 0 of 36
            Pseudo Genes (frameshifted)       :: 2 of 36
            Pseudo Genes (incomplete)         :: 32 of 36
            Pseudo Genes (internal stop)      :: 2 of 36
            ##Genome-Annotation-Data-END##
FEATURES             Location/Qualifiers
     source          1..1881
                     /organism="Micropruina sp."
                     /mol_type="genomic DNA"
                     /submitter_seqid="NODE_25037_length_1881_cov_3.940854"
                     /isolate="AMR_MDS_2195"
                     /isolation_source="Activated sludge of municipal
                     wastewater treatment plants"
                     /db_xref="taxon:2737536"
                     /environmental_sample
                     /geo_loc_name="Canada"
                     /lat_lon="49.25 N 123.1 W"
                     /collection_date="2015-06-16"
                     /metagenome_source="wastewater metagenome"
                     /note="metagenomic"
     gene            complement(<1..726)
                     /locus_tag="PLF56_13715"
     CDS             complement(<1..726)
                     /locus_tag="PLF56_13715"
                     /inference="COORDINATES: protein motif:HMM:NF013757.2"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_function: GO:0003677 - DNA binding [Evidence IEA];
                     GO_function: GO:0003700 - DNA-binding transcription factor
                     activity [Evidence IEA];
                     GO_process: GO:0006355 - regulation of DNA-templated
                     transcription [Evidence IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="IclR family transcriptional regulator"
                     /protein_id="HOQ54655.1"
                     /translation="MDRSAVPALTRAMSIVDLLADAGQSGLTVAELATRTGIAKSTTA
                     LLCTVLESEGMARRHDGRYRLGRRFLGLAAGYLATVDDLEDFYATVRQLPIISREAAR
                     LALLEGTDVIYLARYEAAGRPRRVTGGIGDRFPASITATGKAILATLPPGAVEDRFRG
                     KLFPRYTERSIQSLPDLLRDLDAVRERGYAIDDEETNLGQVCYSVAVQNAAEEVATLA
                     VSSTMTRQRATEVPAADVVDELQQ"
     gene            813..1763
                     /locus_tag="PLF56_13720"
     CDS             813..1763
                     /locus_tag="PLF56_13720"
                     /EC_number="3.5.-.-"
                     /inference="COORDINATES: protein motif:HMM:NF016115.2"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_function: GO:0016812 - hydrolase activity, acting on
                     carbon-nitrogen (but not peptide) bonds, in cyclic amides
                     [Evidence IEA];
                     GO_function: GO:0046872 - metal ion binding [Evidence
                     IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="cyclase family protein"
                     /protein_id="HOQ54656.1"
                     /translation="MNSERVTNPLALPEVPVEQFDEIFDQLKRWGQYSDPSRGAWGTV
                     GPDQVRAAAALVRSGRTAQLGLPWNTVSGIDNPQPALHMMVEMGDREPPEPTCNKDFL
                     GVAFHGKAVSHLDALSHIAYRGQLFDGHSSTEVVHSTGTDFGDVAALRHLVTSAVLLD
                     FAAIDGVDWVEPGTAFHADDILRAEQALGVTIGPGDAVLIRSGHFARRAALGAWNPDV
                     EGSAGLHVDAMPLLADRGISLLGGDGDSDARPAPTPGIGSPIHILAITAMGVPLLDNL
                     DLEELSVACREEGRYTFMLTVAPLNVPRGTGGPVNPIAVF"
BASE COUNT          294 a          652 c          650 g          285 t
ORIGIN      
        1 ttgctgcagc tcgtcgacca cgtcggctgc gggcacctcg gtggcccgct gccgggtcat
       61 cgtcgaactg accgcgaggg tggccacctc ttcggcggcg ttctgcacgg ccaccgagta
      121 gcacacctgc ccgaggttgg tctcctcgtc gtcgatcgcg tacccgcgct cgcgcacggc
      181 gtcgaggtcg cgcagcaggt cgggcagcga ctggatgctt cgctcggtgt agcgcgggaa
      241 caacttgccg cgaaagcggt cttcgaccgc accaggcggc agcgtggcga gaattgcctt
      301 gccggtcgcg gtgatcgagg cagggaagcg gtcaccgata cctccggtga cacggcgcgg
      361 acgtccggcc gcctcgtagc gggccagata gatcacgtcg gtgccttcga gcagtgcgag
      421 ccgggcggcc tcacgactga tgatcggcaa ctggcgcacc gtggcgtaga agtcctccag
      481 gtcgtcgacc gtggccaggt agccggccgc caggccgaga aaacggcggc ccagccggta
      541 ccgcccgtcg tgccggcggg ccatgccctc gctctcgagc accgtgcaca gcagcgcggt
      601 ggtcgacttg gcgatgccgg tgcgggtggc gagttcggcc accgtcaggc ccgattggcc
      661 ggcgtcggca agcaggtcga cgatgctcat cgcccgcgtg agggccggca ctgcgctgcg
      721 gtccatcgga accctccgaa caatatactg gattagtgac agagtaatgg actacggtga
      781 gcacgccaac acccaacgaa gggacgtacg acgtgaattc cgagcgcgtc accaacccgc
      841 ttgccctgcc cgaggtaccg gtcgagcagt tcgacgaaat cttcgaccag ctcaaacggt
      901 ggggacaata tagcgacccc tcgcgggggg cctggggcac cgtcgggccc gatcaggtac
      961 gcgctgcggc cgccctcgtg cgctccggac gcaccgcgca actcggtctg ccctggaaca
     1021 ccgtgagcgg catcgacaac ccgcagccgg cgctgcacat gatggtcgag atgggtgatc
     1081 gcgaaccgcc cgaaccgacc tgcaacaagg actttctcgg cgtcgccttt cacggcaagg
     1141 cggtctcgca cctggacgcg ctgagccaca tcgcctaccg cggccaactc ttcgacggcc
     1201 actcctccac cgaggtggtg cattccaccg gcaccgactt cggcgacgtc gccgccctgc
     1261 ggcacctggt gaccagtgcg gtgctgctcg acttcgccgc gatcgacggg gtggactggg
     1321 tagaaccggg cacggccttc cacgccgacg acattctgcg ggccgagcag gcgctgggcg
     1381 tgacgatcgg gccgggtgat gcggtgctga ttcgcagcgg gcacttcgca cgacgcgccg
     1441 ccctgggtgc ctggaacccc gacgtggagg gcagcgcggg cctgcacgtg gacgccatgc
     1501 cgctgctggc cgatcgcggg atcagcctgc tcggcggcga cggcgacagc gatgcccgcc
     1561 cggcccccac gcccggcatc ggctcaccga tccacattct cgcgatcacc gcgatggggg
     1621 tgccgctgct cgacaacctc gacctggagg agctgtcggt ggcctgtcgc gaggaaggcc
     1681 ggtacacgtt catgctcacc gtcgccccgc tgaacgtgcc gcgcggcacc ggcggaccgg
     1741 tcaacccgat cgcggtcttc tgatgggacg gacgttccgg gtcggggtct cccgcgactt
     1801 cctggacggc gaccgcaacg tgtggggcga catcggcctg cgccggctcg acgacgccgg
     1861 ggtgccgtgg gagtacctgc c
//
LOCUS       DAOSUG010000536         1876 bp    DNA     linear   ENV 10-JUN-2024
DEFINITION  MAG TPA_asm: Micropruina sp. isolate AMR_MDS_2195
            NODE_25135_length_1876_cov_4.752334, whole genome shotgun sequence.
ACCESSION   DAOSUG010000536 DAOSUG010000000
VERSION     DAOSUG010000536.1
DBLINK      BioProject: PRJNA925477
            BioSample: SAMN32864262
            Sequence Read Archive: SRR4236673
KEYWORDS    WGS; ENV; Metagenome Assembled Genome; MAG; Third Party Data; TPA;
            TPA:assembly.
SOURCE      Micropruina sp. (wastewater metagenome)
  ORGANISM  Micropruina sp.
            Bacteria; Bacillati; Actinomycetota; Actinomycetes;
            Propionibacteriales; Nocardioidaceae; Micropruina.
REFERENCE   1  (bases 1 to 1876)
  AUTHORS   Abdulkadir,N., Saraiva,J.P., Zhang,J., Stolte,S., Gillor,O.,
            Harms,H. and Rocha,U.
  TITLE     Genome-centric analyses of 165 metagenomes show that mobile genetic
            elements are crucial for the transmission of antimicrobial
            resistance genes to pathogens in activated sludge and wastewater
  JOURNAL   Microbiol Spectr 12 (3), e0291823 (2024)
   PUBMED   38289113
REFERENCE   2  (bases 1 to 1876)
  AUTHORS   Abdulkadir,N.
  TITLE     Direct Submission
  JOURNAL   Submitted (24-MAR-2023) Environmental Microbiology, Helmholtz
            Center for Environmental Research -UFZ, Permoserstr 15, Leipzig
            04318, Germany
COMMENT     The annotation was added by the NCBI Prokaryotic Genome Annotation
            Pipeline (PGAP). Information about PGAP can be found here:
            https://www.ncbi.nlm.nih.gov/genome/annotation_prok/
            
            ##Genome-Assembly-Data-START##
            Assembly Method        :: SPAdes v. 3.11.1
            Genome Representation  :: Full
            Expected Final Version :: Yes
            Genome Coverage        :: Not Applicable
            Sequencing Technology  :: Illumina MiSeq
            ##Genome-Assembly-Data-END##
            
            ##Genome-Annotation-Data-START##
            Annotation Provider               :: NCBI
            Annotation Date                   :: 05/04/2023 04:57:10
            Annotation Pipeline               :: NCBI Prokaryotic Genome
                                                 Annotation Pipeline (PGAP)
            Annotation Method                 :: Best-placed reference protein
                                                 set; GeneMarkS-2+
            Annotation Software revision      :: 6.5
            Features Annotated                :: Gene; CDS; rRNA; tRNA; ncRNA
            Genes (total)                     :: 2,776
            CDSs (total)                      :: 2,750
            Genes (coding)                    :: 2,714
            CDSs (with protein)               :: 2,714
            Genes (RNA)                       :: 26
            tRNAs                             :: 24
            ncRNAs                            :: 2
            Pseudo Genes (total)              :: 36
            CDSs (without protein)            :: 36
            Pseudo Genes (ambiguous residues) :: 0 of 36
            Pseudo Genes (frameshifted)       :: 2 of 36
            Pseudo Genes (incomplete)         :: 32 of 36
            Pseudo Genes (internal stop)      :: 2 of 36
            ##Genome-Annotation-Data-END##
FEATURES             Location/Qualifiers
     source          1..1876
                     /organism="Micropruina sp."
                     /mol_type="genomic DNA"
                     /submitter_seqid="NODE_25135_length_1876_cov_4.752334"
                     /isolate="AMR_MDS_2195"
                     /isolation_source="Activated sludge of municipal
                     wastewater treatment plants"
                     /db_xref="taxon:2737536"
                     /environmental_sample
                     /geo_loc_name="Canada"
                     /lat_lon="49.25 N 123.1 W"
                     /collection_date="2015-06-16"
                     /metagenome_source="wastewater metagenome"
                     /note="metagenomic"
     gene            complement(16..1395)
                     /locus_tag="PLF56_13725"
     CDS             complement(16..1395)
                     /locus_tag="PLF56_13725"
                     /inference="COORDINATES: protein motif:HMM:NF014724.2"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="ElyC/SanA/YdcF family protein"
                     /protein_id="HOQ54657.1"
                     /translation="MAAVLGTTLSLLSALVISAPARAVDSLSNDSLKTRAIYYLAMSQ
                     PQERDVMIGTLAARTSREGRLWSNFLSTWARINTSMAMHTKVPTGLPAKGHVFVVLGS
                     GLKKSGAVSATFQRRLALAAKAAKAYPTSTILITGGAPRNGRTEAEAGYRWLRKAGVK
                     ASRMLRETRASSTIGNAEYSMAMLARNPRYTTYSLISDSSHLRRASVLFEAAKVLVQE
                     RSGKSWGITRLANVAYPDLPQAGQVPLRTSSVKIAADNVASLFSLLAPYRRLVAKAPA
                     KPTLTSLRLTPPTAVRYQVGQALNRRGLVVRALYDDGVYTRVITSAATITGFDTRTVG
                     GRRATLSYTYRGLTRTARFDYHVVKSGSRATAKASTTSATRSRTRVVLTVTVKPRVTG
                     VQPTGRVRFYLDGARVATVPLSAAKPGVASFRYGTIAAIGSHRLVAAYQGDARVAASS
                     AGVTVTVRK"
BASE COUNT          296 a          590 c          667 g          323 t
ORIGIN      
        1 ccgacggcgt acggctcact ttcgcacggt gaccgttacg ccggccgagg acgcagccac
       61 ccgggcgtcg ccctggtagg ccgcgaccag ccggtgcgag ccgatagcgg cgatggttcc
      121 ataacggaag ctcgccacgc cgggcttggc cgcgctgagc ggcacggtgg cgacgcgcgc
      181 accgtccagg tagaaccgca ccctgccggt cggttgcacc ccggtgaccc ggggcttcac
      241 agtgacggtc agaaccaccc gagtacgcga tcgggttgcg gaggtggtgg atgccttggc
      301 ggtcgccctg ctgcccgact tgaccacgtg gtagtcgaac cgtgcggtgc gggtgagtcc
      361 gcggtaggtg tagctgaggg tggctcgccg gccaccgacc gtccgggtgt cgaaaccggt
      421 gatcgttgcc gccgaggtga tcacacgcgt gtagacgccg tcgtcgtaga gggcgcgaac
      481 caccaggcca cgtcggttca gggcctgccc cacctggtac cgcaccgcgg tcggcggcgt
      541 caggcgcaat gaggtcaagg ttggtttggc aggcgccttg gcgaccaggc gacggtacgg
      601 ggcaagcagc gagaacagcg atgcgacgtt gtcggcggcg atcttcaccg aactggttct
      661 cagcggcacc tggccggctt ggggcagatc gggatatgcc acgttcgcaa gccgggtgat
      721 gccccaactc ttgccggagc gctcctgcac cagtaccttc gccgcctcga acagcaccga
      781 cgcgcggcgc aggtgcgatg agtccgagat caggctgtac gtggtgtatc gggggttgcg
      841 ggccagcatc gccatcgagt actcagcgtt gccgatggtg ctgctggctc gcgtctcgcg
      901 cagcatgcgg gacgccttca cgcccgcctt gcgcagccag cggtagccgg cctcggcctc
      961 ggtgcggccg ttgcgcggcg cgccgcctgt gatcaggatg gtggaggtgg ggtacgcctt
     1021 ggccgccttc gcggccaacg cgagccgccg ctggaaggtt gcgctgaccg cacctgactt
     1081 cttcaggccc gagcccagca cgacgaatac atgacccttg gccggcaatc cggtgggcac
     1141 cttggtatgc atcgccatcg acgtgttgat gcgtgcccac gtgctcagaa agttggacca
     1201 caaccgccct tcgcgacttg ttctggccgc tagtgtgccg atcatcacgt cgcgctcctg
     1261 gggctgcgac atggccaggt agtagatcgc tcgggtcttc aacgaatcat tggagaggct
     1321 gtcgacggcc cgagccgggg cgctgatgac aagggcagaa agcagcgaga gggtggtgcc
     1381 cagaacagcg gccacgagcg gtgctcgaag ggatcgtagg cggctcatca cggggtgacc
     1441 tttacgtcgg gaacagaatc ggcgcgtcga agcgcggcgt ccagacccgg ggaaggtggg
     1501 tgtcaacgcc ggtcgcgaac gctgggcgca ttcgtgacct gcaatggatc ttggcatcgt
     1561 gcacgccgag aaatcgcccc acgttgagac ttcggtggtg ccttgtcgag tgtttgcaga
     1621 gccggttgcg gcaaccgggc cagttctggc cgccggttac tgcgcgtgaa tcaacgcggt
     1681 caccggctgc ggcagcttcg tctgtacggc gaccgctcag ggccgccggt tacgacgcgt
     1741 tcagcagttc ggccaccgct gcggcggctt cgtctgtagg gcgaccggtc aggggccgcc
     1801 ggttacgacg cgttcagcag ttcggccacg gcgccagcgg cgccatctgg tacctcgata
     1861 agtttggttc gactgg
//
LOCUS       DAOSUG010000537         1860 bp    DNA     linear   ENV 10-JUN-2024
DEFINITION  MAG TPA_asm: Micropruina sp. isolate AMR_MDS_2195
            NODE_25439_length_1860_cov_3.812188, whole genome shotgun sequence.
ACCESSION   DAOSUG010000537 DAOSUG010000000
VERSION     DAOSUG010000537.1
DBLINK      BioProject: PRJNA925477
            BioSample: SAMN32864262
            Sequence Read Archive: SRR4236673
KEYWORDS    WGS; ENV; Metagenome Assembled Genome; MAG; Third Party Data; TPA;
            TPA:assembly.
SOURCE      Micropruina sp. (wastewater metagenome)
  ORGANISM  Micropruina sp.
            Bacteria; Bacillati; Actinomycetota; Actinomycetes;
            Propionibacteriales; Nocardioidaceae; Micropruina.
REFERENCE   1  (bases 1 to 1860)
  AUTHORS   Abdulkadir,N., Saraiva,J.P., Zhang,J., Stolte,S., Gillor,O.,
            Harms,H. and Rocha,U.
  TITLE     Genome-centric analyses of 165 metagenomes show that mobile genetic
            elements are crucial for the transmission of antimicrobial
            resistance genes to pathogens in activated sludge and wastewater
  JOURNAL   Microbiol Spectr 12 (3), e0291823 (2024)
   PUBMED   38289113
REFERENCE   2  (bases 1 to 1860)
  AUTHORS   Abdulkadir,N.
  TITLE     Direct Submission
  JOURNAL   Submitted (24-MAR-2023) Environmental Microbiology, Helmholtz
            Center for Environmental Research -UFZ, Permoserstr 15, Leipzig
            04318, Germany
COMMENT     The annotation was added by the NCBI Prokaryotic Genome Annotation
            Pipeline (PGAP). Information about PGAP can be found here:
            https://www.ncbi.nlm.nih.gov/genome/annotation_prok/
            
            ##Genome-Assembly-Data-START##
            Assembly Method        :: SPAdes v. 3.11.1
            Genome Representation  :: Full
            Expected Final Version :: Yes
            Genome Coverage        :: Not Applicable
            Sequencing Technology  :: Illumina MiSeq
            ##Genome-Assembly-Data-END##
            
            ##Genome-Annotation-Data-START##
            Annotation Provider               :: NCBI
            Annotation Date                   :: 05/04/2023 04:57:10
            Annotation Pipeline               :: NCBI Prokaryotic Genome
                                                 Annotation Pipeline (PGAP)
            Annotation Method                 :: Best-placed reference protein
                                                 set; GeneMarkS-2+
            Annotation Software revision      :: 6.5
            Features Annotated                :: Gene; CDS; rRNA; tRNA; ncRNA
            Genes (total)                     :: 2,776
            CDSs (total)                      :: 2,750
            Genes (coding)                    :: 2,714
            CDSs (with protein)               :: 2,714
            Genes (RNA)                       :: 26
            tRNAs                             :: 24
            ncRNAs                            :: 2
            Pseudo Genes (total)              :: 36
            CDSs (without protein)            :: 36
            Pseudo Genes (ambiguous residues) :: 0 of 36
            Pseudo Genes (frameshifted)       :: 2 of 36
            Pseudo Genes (incomplete)         :: 32 of 36
            Pseudo Genes (internal stop)      :: 2 of 36
            ##Genome-Annotation-Data-END##
FEATURES             Location/Qualifiers
     source          1..1860
                     /organism="Micropruina sp."
                     /mol_type="genomic DNA"
                     /submitter_seqid="NODE_25439_length_1860_cov_3.812188"
                     /isolate="AMR_MDS_2195"
                     /isolation_source="Activated sludge of municipal
                     wastewater treatment plants"
                     /db_xref="taxon:2737536"
                     /environmental_sample
                     /geo_loc_name="Canada"
                     /lat_lon="49.25 N 123.1 W"
                     /collection_date="2015-06-16"
                     /metagenome_source="wastewater metagenome"
                     /note="metagenomic"
     gene            complement(<1..1756)
                     /locus_tag="PLF56_13730"
     CDS             complement(<1..1756)
                     /locus_tag="PLF56_13730"
                     /inference="COORDINATES: ab initio
                     prediction:GeneMarkS-2+"
                     /note="Derived by automated computational analysis using
                     gene prediction method: GeneMarkS-2+."
                     /codon_start=1
                     /transl_table=11
                     /product="hypothetical protein"
                     /protein_id="HOQ54658.1"
                     /translation="MSTYANDTNATDYWLGLDGARRDLAARAKERPNACRAIVFFSDG
                     ALDIQRGRSEDDYHRIDRPYADGNPLRTQDQRDAAAQAAEKSLCRAGGLADQIRKQGI
                     IVFGVGLEPANETIDFDLMRRVTTGSHGCGRITKPVPGDFMTGTIDDLLFAFDSAKPG
                     SGQETTAPVCQGSVCAEGAHSFVLDGLVSRVDVLATAPLANVQVFLTAPGGKPVQLPH
                     TEGSTPIPIDGAIGRFRWYSNNTVSIRLTAKKANKWTGQWQLAFVDPTAQSGKGLSRV
                     RVEVTGDIRPVLLDSDGLKQVRQGVPASLRVGVESESGERINRKQILGSLELTADLLT
                     ADGEQRRLWQGDQTNLGDPVQFAPDSYPLGAAILRLTLSATTGPTTVGSRKVPGTALT
                     PTSRDYPLTVLAPADFPAVASTLDFGVLDGTRQANAELPVTGPGCAWLVSGERVVDTS
                     PEEAGAATLQSTKADEQSCLQVPAGQSGMLPVSFQVDAPGNGLVSGTLQVGIASADDP
                     GQRRQVPVAFTAELRKPLNSLNYWSTLIAALVLGLGIPLALVYTIKYLFNSRIPAGAL
                     HAVSCDVELTESGSVLRDG"
BASE COUNT          309 a          616 c          601 g          334 t
ORIGIN      
        1 ttccatcacg cagcaccgac ccactctcgg tgagctcgac gtcgcagctc accgcatgca
       61 gggcgcccgc tggaatccga ctgttgaaga ggtatttgat cgtgtacacc agcgcgaggg
      121 gaatgccgag gccgagcacg agcgcggcaa tcagggtcga ccagtagttc agcgagttga
      181 gcggcttgcg caactcggcg gtgaacgcga ccggcacctg acgccgttgg ccgggatcgt
      241 ccgcggacgc gatcccgacc tgcagcgtcc cgctgaccag tccattgccc ggggcgtcca
      301 cctggaacga aacggggagc atgccggatt gtccggctgg tacctggagg cacgattgct
      361 cgtcggcctt cgtcgactgc aacgttgcgg ctccggcctc ctctggcgag gtgtcgacta
      421 cccgctcgcc agacaccagc cacgcacagc ccgggccggt gaccggcagc tcggcgttgg
      481 cttgtctagt gccatcgagg acgccgaagt cgagggtgct ggccactgcc ggaaagtcgg
      541 ctggagccaa caccgtgagg gggtaatcac gagacgtcgg agtcagcgcc gtgcccggca
      601 ccttgcggct acccacggtg gtcgggcccg ttgtggccga cagcgtcagc cgcaggattg
      661 ccgcgcccag cgggtagctg tcgggggcga actgaaccgg atcaccgaga ttggtctggt
      721 cgccctgcca caatcggcgc tgttctccgt ccgcggtcag caggtccgcg gtcagctcga
      781 gcgaaccgag aatctgcttt ctgttgatcc tttcccccga ctcactctcg acgccgaccc
      841 gcaacgacgc ggggacgccc tggcgcacct gcttgagtcc gtcggaatcg agcagcaccg
      901 gccgaatgtc acccgtcacc tcgacacgca cccggctgag tcccttgccc gactgcgcgg
      961 tcgggtcaac gaaagcgagc tgccactgcc ccgtccattt gttcgccttc ttcgccgtca
     1021 accggatgct gacggtgttg ttcgagtacc acctaaagcg gccgatagct ccgtcgatgg
     1081 ggatgggggt actgccttct gtgtgcggca gttgaacggg cttgccgccc ggcgcggtca
     1141 ggaagacctg cacgttggcc aacggtgctg tcgcaaggac gtcgacccgg ctgaccaggc
     1201 cgtccagcac gaagctgtgc gccccctcgg cgcagacact tccctgacag accggtgccg
     1261 tggtctcctg cccggatccg ggcttcgcgc tatcgaaggc gaacagcagg tcatcgatgg
     1321 tgcccgtcat aaagtcaccc ggtacgggtt tcgtgatgcg cccgcaacca tggctaccgg
     1381 tggtgacacg gcgcatcaga tcgaagtcga tggtctcgtt ggcgggctcc agacccacgc
     1441 cgaagacgat gatgccctgc ttgcggatct gatcggccaa tcctcccgcc cggcacagcg
     1501 acttctccgc tgcttgtgcc gcggcatcgc gttgatcctg ggtccgaagt gggttgccat
     1561 ccgcgtaggg gcgatcgatt cggtggtaat cgtcctcgct gcggccccgc tggatgtcca
     1621 gggccccatc ggagaaaaag acgatcgccc gacatgcgtt cgggcgttct ttggcccgcg
     1681 cggccaagtc gcgacgagcc ccatcgagcc caagccaata gtccgtggcg ttcgtgtcgt
     1741 tggcatacgt gctcacacgt tcggtcagtg cgggcagtgt ggagttgttc agcggacgcc
     1801 actcggtgag cgtctggtat tcgtcgccga aacccgccag ccgaacgccg atcgatacag
//
LOCUS       DAOSUG010000538         1848 bp    DNA     linear   ENV 10-JUN-2024
DEFINITION  MAG TPA_asm: Micropruina sp. isolate AMR_MDS_2195
            NODE_25676_length_1848_cov_4.288901, whole genome shotgun sequence.
ACCESSION   DAOSUG010000538 DAOSUG010000000
VERSION     DAOSUG010000538.1
DBLINK      BioProject: PRJNA925477
            BioSample: SAMN32864262
            Sequence Read Archive: SRR4236673
KEYWORDS    WGS; ENV; Metagenome Assembled Genome; MAG; Third Party Data; TPA;
            TPA:assembly.
SOURCE      Micropruina sp. (wastewater metagenome)
  ORGANISM  Micropruina sp.
            Bacteria; Bacillati; Actinomycetota; Actinomycetes;
            Propionibacteriales; Nocardioidaceae; Micropruina.
REFERENCE   1  (bases 1 to 1848)
  AUTHORS   Abdulkadir,N., Saraiva,J.P., Zhang,J., Stolte,S., Gillor,O.,
            Harms,H. and Rocha,U.
  TITLE     Genome-centric analyses of 165 metagenomes show that mobile genetic
            elements are crucial for the transmission of antimicrobial
            resistance genes to pathogens in activated sludge and wastewater
  JOURNAL   Microbiol Spectr 12 (3), e0291823 (2024)
   PUBMED   38289113
REFERENCE   2  (bases 1 to 1848)
  AUTHORS   Abdulkadir,N.
  TITLE     Direct Submission
  JOURNAL   Submitted (24-MAR-2023) Environmental Microbiology, Helmholtz
            Center for Environmental Research -UFZ, Permoserstr 15, Leipzig
            04318, Germany
COMMENT     The annotation was added by the NCBI Prokaryotic Genome Annotation
            Pipeline (PGAP). Information about PGAP can be found here:
            https://www.ncbi.nlm.nih.gov/genome/annotation_prok/
            
            ##Genome-Assembly-Data-START##
            Assembly Method        :: SPAdes v. 3.11.1
            Genome Representation  :: Full
            Expected Final Version :: Yes
            Genome Coverage        :: Not Applicable
            Sequencing Technology  :: Illumina MiSeq
            ##Genome-Assembly-Data-END##
            
            ##Genome-Annotation-Data-START##
            Annotation Provider               :: NCBI
            Annotation Date                   :: 05/04/2023 04:57:10
            Annotation Pipeline               :: NCBI Prokaryotic Genome
                                                 Annotation Pipeline (PGAP)
            Annotation Method                 :: Best-placed reference protein
                                                 set; GeneMarkS-2+
            Annotation Software revision      :: 6.5
            Features Annotated                :: Gene; CDS; rRNA; tRNA; ncRNA
            Genes (total)                     :: 2,776
            CDSs (total)                      :: 2,750
            Genes (coding)                    :: 2,714
            CDSs (with protein)               :: 2,714
            Genes (RNA)                       :: 26
            tRNAs                             :: 24
            ncRNAs                            :: 2
            Pseudo Genes (total)              :: 36
            CDSs (without protein)            :: 36
            Pseudo Genes (ambiguous residues) :: 0 of 36
            Pseudo Genes (frameshifted)       :: 2 of 36
            Pseudo Genes (incomplete)         :: 32 of 36
            Pseudo Genes (internal stop)      :: 2 of 36
            ##Genome-Annotation-Data-END##
FEATURES             Location/Qualifiers
     source          1..1848
                     /organism="Micropruina sp."
                     /mol_type="genomic DNA"
                     /submitter_seqid="NODE_25676_length_1848_cov_4.288901"
                     /isolate="AMR_MDS_2195"
                     /isolation_source="Activated sludge of municipal
                     wastewater treatment plants"
                     /db_xref="taxon:2737536"
                     /environmental_sample
                     /geo_loc_name="Canada"
                     /lat_lon="49.25 N 123.1 W"
                     /collection_date="2015-06-16"
                     /metagenome_source="wastewater metagenome"
                     /note="metagenomic"
     gene            complement(<1..896)
                     /locus_tag="PLF56_13735"
     CDS             complement(<1..896)
                     /locus_tag="PLF56_13735"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_008357328.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="glycoside hydrolase family 16 protein"
                     /protein_id="HOQ54659.1"
                     /translation="MAVATAVLLASTGGLLGAGSAQAAPVANGWTTVWSDDFNGAAGT
                     LPGPDWQIDTGTSYPGGPPAWGTWEVQTYTDSTANLFQDGNGSLNIKPLLDSSGKWTS
                     ARIETKRSNFKPAAGGVLAIESRIQMPNVTGDKALGYWPAFWALGSPYRGNWWNWPGI
                     GELDIMENVNGMNRVWGVLHCGVNPGGPCNESTGIAAFRECPGASCQSAFHTYRFEWD
                     RSVTPNQLRWYVDGQQWHGVSQDQVPADTWTSMTSHEGYYVLLNVAMGGAFPNALAGE
                     TPTAATEPGHAMLVDYVSVQAKG"
     gene            1124..>1848
                     /locus_tag="PLF56_13740"
     CDS             1124..>1848
                     /locus_tag="PLF56_13740"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_015588727.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="hypothetical protein"
                     /protein_id="HOQ54660.1"
                     /translation="MVETGTMPTDVTAAAVNLRLALIGIQVPLQAEEATSAQLVAPIL
                     ARQRELSRRLSDRPCAADQRIQAFLDDYLAEVYPGDLAHEGPTLPRRTLVLDEPGLAR
                     ALSLPVNADSFSSPLLSSYRLANGVLHNPANDRRTTAGVFHIAEGGLPIPDDKIAVPK
                     SVFGRLLALALQPPEGDLVLPYLSRTDHPAACFVSLLLRPLVSPAVPGYATERRMETR
                     FIVPGGLVANLDFVEGIFGNGGDP"
BASE COUNT          285 a          670 c          583 g          310 t
ORIGIN      
        1 cccttggcct gcacgctcac gtagtcgacc agcatggcgt gacccggctc ggtcgcagcg
       61 gtcggcgtct cgccggccag cgcattcggg aaggccccac ccatggccac gttcagcagt
      121 acgtagtagc cctcgtggct ggtcatgctg gtccaggtgt cggccggcac ctggtcctgc
      181 gacacaccgt gccactgctg gccgtccacg taccagcgca gctggttggg cgtcaccgac
      241 cggtcccatt cgaagcggta ggtgtggaac gccgactggc aggacgctcc cgggcactcc
      301 cggaacgccg cgatgccggt gctctcgttg cacggtccac ccgggttgac gccgcagtgc
      361 agcacccccc agacgcggtt catgccgttg acgttctcca tgatgtcgag ctcgccgatg
      421 cccggccagt tccaccagtt gccccggtac gggctgccca acgcccagaa cgccggccag
      481 tacccgagcg ccttgtcacc ggtgacgttg ggcatctgaa tgcgcgactc gatcgccagc
      541 acgccccccg cggccggctt gaagttggag cgcttggtct cgatgcgtgc cgaggtccat
      601 ttgccggacg aatccagcaa cggcttgatg ttcaggcttc cgttgccgtc ctggaacagg
      661 ttggcggtcg aatcggtgta ggtctgcacc tcccaggtac cccaggcggg cggtccgccg
      721 gggtaggagg tgccggtatc gatctgccag tccgggcccg gcagggtgcc ggcggcaccg
      781 ttgaaatcgt cggaccagac ggtggtccag ccgttggcca cgggagcggc ctgagcactg
      841 cctgcgccga gaagtccgcc ggttgaggcc agcagcacgg cggtggcaac cgccaccgac
      901 cgcttgtgct tggctttcga tgaacctctg cggggggcgc gcaacaacag gtgcatttcg
      961 tgtctccttt gacaccgtgg gggatgatct gccaatgaaa gcgctttcaa gcgacaaggt
     1021 caatagcttg cgacgatcga tccgaaatgc acagaagacg tgacacccac cggcaggtaa
     1081 cagtctgcgg tcgaggttga gacctgctga caagccacgg aacatggtcg agactggaac
     1141 catgcccacc gacgtgaccg ctgccgccgt caacctgcgc ctcgccctga tcggcatcca
     1201 ggtgcccctg caggcagaag aggccaccag cgctcaactg gtcgccccga tcctggcccg
     1261 ccagcgcgaa ctcagccgac gcctgtccga ccggccgtgc gccgccgatc agcgcatcca
     1321 ggcgttcctc gacgactacc tggccgaggt gtaccccggt gacctcgccc acgagggtcc
     1381 cactctgccc cggcgcaccc tggtgctgga cgaacccggc ctggcccgcg ccctgtcgct
     1441 tccggtcaac gccgactcgt tcagctcgcc gctgctgtcg agctaccggc tcgcgaacgg
     1501 cgtgctgcac aaccccgcca acgaccgccg caccaccgcc ggcgtctttc acatcgccga
     1561 gggcgggctg cccatccccg acgacaagat cgcggtgccc aagtccgttt tcggacgcct
     1621 gctcgccctc gccctgcagc cccccgaagg cgacctggtg ctgccctacc tgtcgcgcac
     1681 cgatcatccg gccgcctgct tcgtgtcgct gctgctgcgt ccgctggtgt cgccggctgt
     1741 gcccggctac gccacggagc gtcgcatgga gacccggttc atcgtgccgg gcggcctggt
     1801 cgccaacctc gacttcgtcg agggcatctt cggcaacggc ggcgaccc
//
LOCUS       DAOSUG010000539         1844 bp    DNA     linear   ENV 10-JUN-2024
DEFINITION  MAG TPA_asm: Micropruina sp. isolate AMR_MDS_2195
            NODE_25768_length_1844_cov_3.726104, whole genome shotgun sequence.
ACCESSION   DAOSUG010000539 DAOSUG010000000
VERSION     DAOSUG010000539.1
DBLINK      BioProject: PRJNA925477
            BioSample: SAMN32864262
            Sequence Read Archive: SRR4236673
KEYWORDS    WGS; ENV; Metagenome Assembled Genome; MAG; Third Party Data; TPA;
            TPA:assembly.
SOURCE      Micropruina sp. (wastewater metagenome)
  ORGANISM  Micropruina sp.
            Bacteria; Bacillati; Actinomycetota; Actinomycetes;
            Propionibacteriales; Nocardioidaceae; Micropruina.
REFERENCE   1  (bases 1 to 1844)
  AUTHORS   Abdulkadir,N., Saraiva,J.P., Zhang,J., Stolte,S., Gillor,O.,
            Harms,H. and Rocha,U.
  TITLE     Genome-centric analyses of 165 metagenomes show that mobile genetic
            elements are crucial for the transmission of antimicrobial
            resistance genes to pathogens in activated sludge and wastewater
  JOURNAL   Microbiol Spectr 12 (3), e0291823 (2024)
   PUBMED   38289113
REFERENCE   2  (bases 1 to 1844)
  AUTHORS   Abdulkadir,N.
  TITLE     Direct Submission
  JOURNAL   Submitted (24-MAR-2023) Environmental Microbiology, Helmholtz
            Center for Environmental Research -UFZ, Permoserstr 15, Leipzig
            04318, Germany
COMMENT     The annotation was added by the NCBI Prokaryotic Genome Annotation
            Pipeline (PGAP). Information about PGAP can be found here:
            https://www.ncbi.nlm.nih.gov/genome/annotation_prok/
            
            ##Genome-Assembly-Data-START##
            Assembly Method        :: SPAdes v. 3.11.1
            Genome Representation  :: Full
            Expected Final Version :: Yes
            Genome Coverage        :: Not Applicable
            Sequencing Technology  :: Illumina MiSeq
            ##Genome-Assembly-Data-END##
            
            ##Genome-Annotation-Data-START##
            Annotation Provider               :: NCBI
            Annotation Date                   :: 05/04/2023 04:57:10
            Annotation Pipeline               :: NCBI Prokaryotic Genome
                                                 Annotation Pipeline (PGAP)
            Annotation Method                 :: Best-placed reference protein
                                                 set; GeneMarkS-2+
            Annotation Software revision      :: 6.5
            Features Annotated                :: Gene; CDS; rRNA; tRNA; ncRNA
            Genes (total)                     :: 2,776
            CDSs (total)                      :: 2,750
            Genes (coding)                    :: 2,714
            CDSs (with protein)               :: 2,714
            Genes (RNA)                       :: 26
            tRNAs                             :: 24
            ncRNAs                            :: 2
            Pseudo Genes (total)              :: 36
            CDSs (without protein)            :: 36
            Pseudo Genes (ambiguous residues) :: 0 of 36
            Pseudo Genes (frameshifted)       :: 2 of 36
            Pseudo Genes (incomplete)         :: 32 of 36
            Pseudo Genes (internal stop)      :: 2 of 36
            ##Genome-Annotation-Data-END##
FEATURES             Location/Qualifiers
     source          1..1844
                     /organism="Micropruina sp."
                     /mol_type="genomic DNA"
                     /submitter_seqid="NODE_25768_length_1844_cov_3.726104"
                     /isolate="AMR_MDS_2195"
                     /isolation_source="Activated sludge of municipal
                     wastewater treatment plants"
                     /db_xref="taxon:2737536"
                     /environmental_sample
                     /geo_loc_name="Canada"
                     /lat_lon="49.25 N 123.1 W"
                     /collection_date="2015-06-16"
                     /metagenome_source="wastewater metagenome"
                     /note="metagenomic"
     gene            <1..1235
                     /locus_tag="PLF56_13745"
     CDS             <1..1235
                     /locus_tag="PLF56_13745"
                     /inference="COORDINATES: protein motif:HMM:NF023326.2"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=3
                     /transl_table=11
                     /product="DUF3418 domain-containing protein"
                     /protein_id="HOQ54661.1"
                     /translation="DFGWQVPGLRGELVTALVRGLPKQWRARLVPAPDTARRAVAWLD
                     EHRVAGESLTDGLGRAIQALTGVQVPADAWQLDSVPEHLRVRFVVTRDGAEVATGKDL
                     PALAETLGSKVRAELNAEASEHTHAGSRQWAFGTVAERVDGAVVGYPALSEVGDLVGV
                     TVYATTSEARRSHLLGLRRLIASTSPDPTNWVVSRLSNPVKFALAASPYPTVPALLAD
                     ARLKAIETLTLAAGDPWGVRAEGAFTALRDRVRAEAADRMLAVVNTAGEVLQRHQQVQ
                     LALPAAPATVRSDVTEQVAGLVYRGFIAGTPDPYWQRLPRYLHAIELRLAAARANPAR
                     DRVNAEVIEPLEDEYAALCAGYPAGPLPQDVANVGWLLEELRVSLFAQSLGTAAPVSA
                     KRVRTAMATVAPPRRTPA"
     gene            complement(1296..>1844)
                     /locus_tag="PLF56_13750"
     CDS             complement(1296..>1844)
                     /locus_tag="PLF56_13750"
                     /inference="COORDINATES: protein motif:HMM:NF012335.2"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="zinc-binding dehydrogenase"
                     /protein_id="HOQ54662.1"
                     /translation="PGATVLIHGATGVSGLLAVQVAKTLGAGQVVAAGRSRSGLARAK
                     DRGADALLALDEPLAPQLEQAAPDGVDLVVDYLWGERTRNLLVALLTTERKPDSVLEL
                     IEVGSMVGPWMPLDAAWLRSRPLRISGSGLGSVGAEAMFRAVGGVLQAAAEGRLSIDF
                     VTRPLSEVQDAWAMKERLVLIP"
BASE COUNT          252 a          660 c          671 g          261 t
ORIGIN      
        1 acgatttcgg ctggcaggtt ccgggccttc gtggcgaact ggtcacggca ctcgtccgcg
       61 ggttgccgaa gcaatggcgg gcccggctgg tgccggcccc cgacaccgcg cgccgcgccg
      121 tcgcgtggct ggatgagcac cgggtggcgg gagagtcgct caccgacggg ctgggccggg
      181 cgatccaggc cctgaccggg gtgcaggtac ccgccgacgc ctggcagctc gactcggtgc
      241 ccgagcacct gcgggtgcgc ttcgtggtca cccgggacgg cgccgaggtg gccaccggaa
      301 aggacctgcc cgcgctggcc gagacgctcg gatcgaaggt gcgggccgaa ctgaacgccg
      361 aggcgtccga gcacacccat gcggggtcga ggcagtgggc cttcggcacc gtcgccgagc
      421 gggtggacgg tgctgtcgtc gggtatccgg ccttgtcgga ggtgggcgat ctggtcggtg
      481 tgaccgtgta cgcgacgacg tccgaggccc ggcgatcgca cctgctgggc ctgcgccgtc
      541 tcattgcgtc gaccagcccc gaccccacca actgggtcgt ctcgcggctg agcaacccgg
      601 tcaagttcgc gctggccgcc tcgccctacc cgacggtgcc ggcactgctg gccgatgcgc
      661 ggttgaaggc gatcgagacc ctgaccctcg ccgccggcga cccgtggggc gtgcgtgctg
      721 agggggcgtt caccgctttg cgcgaccggg tgcgtgccga ggccgccgac cggatgctgg
      781 ccgtggtgaa caccgccggc gaggtgttgc agcgacacca gcaggtgcag ctcgcgttgc
      841 cggcggctcc ggcgacggtt cgaagcgacg tgaccgagca ggtcgccggc ctggtctacc
      901 gcggcttcat cgcgggtacc cccgatccgt attggcagcg gctgccgcgc tacctgcacg
      961 cgatcgagct gaggctggcg gccgcacggg cgaaccccgc gcgcgaccgg gtcaacgccg
     1021 aagtaatcga gccgctggag gacgagtacg ccgcgctgtg tgcgggctat ccggcggggc
     1081 cgttgccgca ggacgtggcg aacgtcggct ggctgctgga agaactgcgg gtcagcctgt
     1141 tcgcccaatc gctgggcacc gcggcgccgg tctcggccaa gcgggtccgg acggcgatgg
     1201 ccaccgtggc gcccccgcgc cgaacccccg cctgactttc tgcggcctgc gcaactccgc
     1261 ggagttgcgc aggccgcaga aagttgggca ggggttcagg gaatcagcac caggcgctcc
     1321 ttcatggccc acgcgtcctg cacctcggac aacgggcggg tgacgaagtc gatgctcaac
     1381 cgtccctcag cggccgcctg cagcacgccg ccgacggccc ggaacatggc ttcggcgccc
     1441 acgctgccca ggccgctgcc gctgatccgc agcggacggc tgcgcagcca ggctgcgtcc
     1501 agtggcatcc aggggccgac catggagccg acctcgatca gctcgaggac gctgtccggt
     1561 ttccgttccg tcgtcagcag ggccacaagc aggttgcggg tgcgctcgcc ccacaggtag
     1621 tcgacgacca ggtcgacccc atctggggct gcctgctcca actgcggtgc cagcggctca
     1681 tccagggcga ggagtgcatc cgcgccgcgg tccttcgccc gcgcgagtcc cgatcgggag
     1741 cggcccgccg cgaccacctg tccggcgccc aacgtcttgg ccacctgcac cgccagcaag
     1801 cccgagacgc cggtcgcgcc atggatcagc accgtcgcgc ccgg
//
LOCUS       DAOSUG010000540         1836 bp    DNA     linear   ENV 10-JUN-2024
DEFINITION  MAG TPA_asm: Micropruina sp. isolate AMR_MDS_2195
            NODE_25933_length_1836_cov_3.015160, whole genome shotgun sequence.
ACCESSION   DAOSUG010000540 DAOSUG010000000
VERSION     DAOSUG010000540.1
DBLINK      BioProject: PRJNA925477
            BioSample: SAMN32864262
            Sequence Read Archive: SRR4236673
KEYWORDS    WGS; ENV; Metagenome Assembled Genome; MAG; Third Party Data; TPA;
            TPA:assembly.
SOURCE      Micropruina sp. (wastewater metagenome)
  ORGANISM  Micropruina sp.
            Bacteria; Bacillati; Actinomycetota; Actinomycetes;
            Propionibacteriales; Nocardioidaceae; Micropruina.
REFERENCE   1  (bases 1 to 1836)
  AUTHORS   Abdulkadir,N., Saraiva,J.P., Zhang,J., Stolte,S., Gillor,O.,
            Harms,H. and Rocha,U.
  TITLE     Genome-centric analyses of 165 metagenomes show that mobile genetic
            elements are crucial for the transmission of antimicrobial
            resistance genes to pathogens in activated sludge and wastewater
  JOURNAL   Microbiol Spectr 12 (3), e0291823 (2024)
   PUBMED   38289113
REFERENCE   2  (bases 1 to 1836)
  AUTHORS   Abdulkadir,N.
  TITLE     Direct Submission
  JOURNAL   Submitted (24-MAR-2023) Environmental Microbiology, Helmholtz
            Center for Environmental Research -UFZ, Permoserstr 15, Leipzig
            04318, Germany
COMMENT     The annotation was added by the NCBI Prokaryotic Genome Annotation
            Pipeline (PGAP). Information about PGAP can be found here:
            https://www.ncbi.nlm.nih.gov/genome/annotation_prok/
            
            ##Genome-Assembly-Data-START##
            Assembly Method        :: SPAdes v. 3.11.1
            Genome Representation  :: Full
            Expected Final Version :: Yes
            Genome Coverage        :: Not Applicable
            Sequencing Technology  :: Illumina MiSeq
            ##Genome-Assembly-Data-END##
            
            ##Genome-Annotation-Data-START##
            Annotation Provider               :: NCBI
            Annotation Date                   :: 05/04/2023 04:57:10
            Annotation Pipeline               :: NCBI Prokaryotic Genome
                                                 Annotation Pipeline (PGAP)
            Annotation Method                 :: Best-placed reference protein
                                                 set; GeneMarkS-2+
            Annotation Software revision      :: 6.5
            Features Annotated                :: Gene; CDS; rRNA; tRNA; ncRNA
            Genes (total)                     :: 2,776
            CDSs (total)                      :: 2,750
            Genes (coding)                    :: 2,714
            CDSs (with protein)               :: 2,714
            Genes (RNA)                       :: 26
            tRNAs                             :: 24
            ncRNAs                            :: 2
            Pseudo Genes (total)              :: 36
            CDSs (without protein)            :: 36
            Pseudo Genes (ambiguous residues) :: 0 of 36
            Pseudo Genes (frameshifted)       :: 2 of 36
            Pseudo Genes (incomplete)         :: 32 of 36
            Pseudo Genes (internal stop)      :: 2 of 36
            ##Genome-Annotation-Data-END##
FEATURES             Location/Qualifiers
     source          1..1836
                     /organism="Micropruina sp."
                     /mol_type="genomic DNA"
                     /submitter_seqid="NODE_25933_length_1836_cov_3.015160"
                     /isolate="AMR_MDS_2195"
                     /isolation_source="Activated sludge of municipal
                     wastewater treatment plants"
                     /db_xref="taxon:2737536"
                     /environmental_sample
                     /geo_loc_name="Canada"
                     /lat_lon="49.25 N 123.1 W"
                     /collection_date="2015-06-16"
                     /metagenome_source="wastewater metagenome"
                     /note="metagenomic"
     gene            complement(<1..654)
                     /locus_tag="PLF56_13755"
     CDS             complement(<1..654)
                     /locus_tag="PLF56_13755"
                     /inference="COORDINATES: protein motif:HMM:NF014477.2"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="FAD:protein FMN transferase"
                     /protein_id="HOQ54663.1"
                     /translation="MTTPVLDLPRRAYVAQIMGLPVSIHLRGPHARGLTAAAAVERAF
                     DALRADDAMFSTRKPDSPVSRIRDGRDQLSDAHPRIRHVAALCELAAHRTGGAFCAWL
                     PGPHSRPRFDPTGLVKGWAVEQAFSRLLNELGPLGPHDAMINAGGDIAVHSSRTDTPD
                     WTIGIEDPRDRTRVLRTLTLRTGAIATSGVDARGRHITDPATGRPASGLLSATVIGPE
                     "
     gene            complement(651..1142)
                     /locus_tag="PLF56_13760"
     CDS             complement(651..1142)
                     /locus_tag="PLF56_13760"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_013860922.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_component: GO:0016020 - membrane [Evidence IEA];
                     GO_function: GO:0010181 - FMN binding [Evidence IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="FMN-binding protein"
                     /protein_id="HOQ54664.1"
                     /translation="MRKIVVAAMSTISGLILLFSYHTSTNSTAATTTGTDAGSEPTAG
                     GGTTPNAGTATTPSVPSASASPSSGTSSGTFTGDAVDTRWGTVQVRITVKDGTITAAD
                     AIEYPTANPKDQQINAYAIPQLNAEAVDAQSTNIDAVSGATVTSDGYIESLQSAIDQA
                     HLK"
     gene            complement(1166..>1836)
                     /locus_tag="PLF56_13765"
     CDS             complement(1166..>1836)
                     /locus_tag="PLF56_13765"
                     /inference="COORDINATES: protein motif:HMM:NF012402.2"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=3
                     /transl_table=11
                     /product="FAD-binding oxidoreductase"
                     /protein_id="HOQ54665.1"
                     /translation="SPDVVSVTIGGRNLERLQASAGQFFNWRFMTGPGWMRAHPYSLS
                     AAPDGGTLRITVKDLGDGSHALAALRPGTRVLIEGPYGRLHPGVRTRPKVTLIAAGIG
                     ISPIRSLFEALPQGPGDVTLIYRARTQADLVLRAEIDTLARARGARVFHILGRRIPGR
                     DTWLPEAAAHLTDAQALQQLVPDIADQDVYLCGASAWMDAAERAALDCGVPAARIHTE
                     RFSW"
BASE COUNT          235 a          571 c          716 g          314 t
ORIGIN      
        1 ttcgggcccg atgacggtgg ccgacagaag gccggacgcg ggccgtccgg tggccgggtc
       61 ggtgatgtgc cgacctcgcg cgtccactcc ggaggtggcg atggcgccgg tgcgcagggt
      121 gagcgtgcgc agcaccctgg tgcggtcgcg ggggtcctcg atgccgatgg tccaatccgg
      181 ggtgtccgtg cggctgctgt ggacggcgat gtcgccgccg gcgttgatca tggcgtcgtg
      241 tgggccgagg gggcccaact cgttgaggag ccgcgagaag gcctgctcga cggcccagcc
      301 cttgaccagg ccggtgggat cgaagcgggg ccggctgtgg ggtccgggaa gccaggcgca
      361 gaaggcgccg ccggtgcggt gggctgcgag ttcgcagagg gctgcgacgt gcctgattcg
      421 ggggtgggcg tcagacagct ggtcgcgtcc gtcgcggatc cggctgaccg gactgtcggg
      481 cttccgggtg ctgaacatcg cgtcgtcggc gcgcagcgcg tcgaacgccc gctcgacagc
      541 tgccgcagcg gtcaggccac gagcgtgggg gccccgtaga tggatgctga ccggcaggcc
      601 catgatctgt gccacgtagg cccgtctggg caggtcgagc accggtgtgg tcacttgagg
      661 tgcgcctggt cgatggcgga ctgcagtgac tcgatgtagc cgtcgctggt caccgtggcg
      721 ccagagacgg catcgatgtt cgtggactgg gcatcgacgg cctcggcgtt caactgcggg
      781 atcgcgtacg cattgatctg ctgatccttc ggattcgccg tcgggtactc gatggcgtct
      841 gcagcggtga tcgtgccgtc cttgacggtg atccgaacct ggacggtgcc ccagcgggtg
      901 tcgacggcgt caccggtgaa ggttccgctg ctcgtcccgg acgacgggga cgccgaggcc
      961 gagggaaccg acggtgtggt ggcggtgccg gcattgggtg tggtgccgcc cccggccgtc
     1021 ggttcgcttc cggcgtccgt tccggttgtg gtggcggctg tgctgttggt gctggtgtga
     1081 tagctgaaca gcagaatcag gccgctgatg gtgctcatcg cggcgacgac gatcttgcgc
     1141 atggtgttcc tttcgggcgg ccgggttacc agctgaaccg ttcggtgtgg atccgcgcgg
     1201 ccgggacgcc gcagtcgaga gcggcccgtt cggcggcgtc catccaggcc gaggcgccgc
     1261 acaggtagac gtcctggtct gcgatgtcgg gtacgagttg ttgcaacgcc tgcgcgtcgg
     1321 tgaggtgtgc ggcggcttcg ggcagccagg tgtcgcgtcc gggtatccgc cgtccgagga
     1381 tgtggaacac ccgggcgccc cgggcccggg cgagcgtgtc gatctcggcg cgcagcacca
     1441 ggtcggcctg ggtgcgtgcc cgatagatga gggtcacgtc tccgggcccc tgcgggagcg
     1501 cctcgaacag ggaacggatc gggctgattc cgatcccggc ggcgatcagg gtgaccttcg
     1561 gccgggttcg cacgccggga tgcaaccgcc cgtaggggcc ctcgatgagt acccgcgtgc
     1621 ccggccggag cgcggccagc gcatggctgc cgtcgcccag gtccttcacg gtgatgcgca
     1681 gcgtcccgcc gtccggtgcc gccgaaagcg agtacgggtg ggcccgcatc caacccggcc
     1741 cggtcatgaa ccgccagttg aagaactggc cggccgatgc ttgcagccgc tccaggttcc
     1801 ggccaccgat ggtgaccgac accacgtccg ggctct
//
LOCUS       DAOSUG010000541         1826 bp    DNA     linear   ENV 10-JUN-2024
DEFINITION  MAG TPA_asm: Micropruina sp. isolate AMR_MDS_2195
            NODE_26133_length_1826_cov_2.823828, whole genome shotgun sequence.
ACCESSION   DAOSUG010000541 DAOSUG010000000
VERSION     DAOSUG010000541.1
DBLINK      BioProject: PRJNA925477
            BioSample: SAMN32864262
            Sequence Read Archive: SRR4236673
KEYWORDS    WGS; ENV; Metagenome Assembled Genome; MAG; Third Party Data; TPA;
            TPA:assembly.
SOURCE      Micropruina sp. (wastewater metagenome)
  ORGANISM  Micropruina sp.
            Bacteria; Bacillati; Actinomycetota; Actinomycetes;
            Propionibacteriales; Nocardioidaceae; Micropruina.
REFERENCE   1  (bases 1 to 1826)
  AUTHORS   Abdulkadir,N., Saraiva,J.P., Zhang,J., Stolte,S., Gillor,O.,
            Harms,H. and Rocha,U.
  TITLE     Genome-centric analyses of 165 metagenomes show that mobile genetic
            elements are crucial for the transmission of antimicrobial
            resistance genes to pathogens in activated sludge and wastewater
  JOURNAL   Microbiol Spectr 12 (3), e0291823 (2024)
   PUBMED   38289113
REFERENCE   2  (bases 1 to 1826)
  AUTHORS   Abdulkadir,N.
  TITLE     Direct Submission
  JOURNAL   Submitted (24-MAR-2023) Environmental Microbiology, Helmholtz
            Center for Environmental Research -UFZ, Permoserstr 15, Leipzig
            04318, Germany
COMMENT     The annotation was added by the NCBI Prokaryotic Genome Annotation
            Pipeline (PGAP). Information about PGAP can be found here:
            https://www.ncbi.nlm.nih.gov/genome/annotation_prok/
            
            ##Genome-Assembly-Data-START##
            Assembly Method        :: SPAdes v. 3.11.1
            Genome Representation  :: Full
            Expected Final Version :: Yes
            Genome Coverage        :: Not Applicable
            Sequencing Technology  :: Illumina MiSeq
            ##Genome-Assembly-Data-END##
            
            ##Genome-Annotation-Data-START##
            Annotation Provider               :: NCBI
            Annotation Date                   :: 05/04/2023 04:57:10
            Annotation Pipeline               :: NCBI Prokaryotic Genome
                                                 Annotation Pipeline (PGAP)
            Annotation Method                 :: Best-placed reference protein
                                                 set; GeneMarkS-2+
            Annotation Software revision      :: 6.5
            Features Annotated                :: Gene; CDS; rRNA; tRNA; ncRNA
            Genes (total)                     :: 2,776
            CDSs (total)                      :: 2,750
            Genes (coding)                    :: 2,714
            CDSs (with protein)               :: 2,714
            Genes (RNA)                       :: 26
            tRNAs                             :: 24
            ncRNAs                            :: 2
            Pseudo Genes (total)              :: 36
            CDSs (without protein)            :: 36
            Pseudo Genes (ambiguous residues) :: 0 of 36
            Pseudo Genes (frameshifted)       :: 2 of 36
            Pseudo Genes (incomplete)         :: 32 of 36
            Pseudo Genes (internal stop)      :: 2 of 36
            ##Genome-Annotation-Data-END##
FEATURES             Location/Qualifiers
     source          1..1826
                     /organism="Micropruina sp."
                     /mol_type="genomic DNA"
                     /submitter_seqid="NODE_26133_length_1826_cov_2.823828"
                     /isolate="AMR_MDS_2195"
                     /isolation_source="Activated sludge of municipal
                     wastewater treatment plants"
                     /db_xref="taxon:2737536"
                     /environmental_sample
                     /geo_loc_name="Canada"
                     /lat_lon="49.25 N 123.1 W"
                     /collection_date="2015-06-16"
                     /metagenome_source="wastewater metagenome"
                     /note="metagenomic"
     gene            <1..641
                     /locus_tag="PLF56_13770"
     CDS             <1..641
                     /locus_tag="PLF56_13770"
                     /inference="COORDINATES: protein motif:HMM:NF012738.2"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_process: GO:0008643 - carbohydrate transport [Evidence
                     IEA];
                     GO_process: GO:0055085 - transmembrane transport [Evidence
                     IEA]"
                     /codon_start=3
                     /transl_table=11
                     /product="carbohydrate ABC transporter permease"
                     /protein_id="HOQ54666.1"
                     /translation="VMLLRILLCLVFGLPLVFLIVSSFKPDSAIFGDLGSINAFLPIG
                     QLSLDNYTGVFDRVPFATFLLNSALISVVTVVFGLIVNSMAAFALSRMEFKGQKVLLG
                     VILATLIVPFETMALPLLWWVNQLPYFDGSTGWLDTYEVQIIPFIANAFSIYLFYQYF
                     DSIPKELDEAAKVDGAGWFRIYRSIVMPLSGPAVATVAILTSCRLGTPTCGR"
     gene            641..838
                     /locus_tag="PLF56_13775"
     CDS             641..838
                     /locus_tag="PLF56_13775"
                     /inference="COORDINATES: ab initio
                     prediction:GeneMarkS-2+"
                     /note="Derived by automated computational analysis using
                     gene prediction method: GeneMarkS-2+."
                     /codon_start=1
                     /transl_table=11
                     /product="hypothetical protein"
                     /protein_id="HOQ54667.1"
                     /translation="MVTQSEAIRPAMVGMDYFKQMTGATGTAWGQIMAYASMITVPVL
                     VLFIAFQRAFINSIASSGVKG"
     gene            845..1789
                     /locus_tag="PLF56_13780"
     CDS             845..1789
                     /locus_tag="PLF56_13780"
                     /inference="COORDINATES: protein motif:HMM:NF012475.2"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="hypothetical protein"
                     /protein_id="HOQ54668.1"
                     /translation="MFDPPEAWVWDFWVVDDGEQYHLFFLYASRALKNPEARHYRASV
                     GHAISSDLTCWTRVTDALVRSDVPAPDDLATWTGSVIQGPDGLWYLFYTGATAAPNGA
                     NVQTICYATSPDLYEWTKAPGPILRADPRWYELLSDGQWHDEAFRDPFVFADPAGNGW
                     HLLITARANHGPSDDRGVVGHAWSPNLTDWELRPPLSRPGQGYGQLEVFELVRLQGRD
                     YLVFNCLAGDLSAAHRASGTTGGVWLAAAESPLGPYDLAGAQLVTGDDLYVGRFVTAR
                     SGAPVFLAFRNAGPGGTFMGGITDPLPVSVVNGSVSVG"
BASE COUNT          252 a          605 c          609 g          360 t
ORIGIN      
        1 tggtgatgct gttgcgcatc cttctctgcc tggtgttcgg cctgccgctg gtgttcctga
       61 tcgtcagcag cttcaagccc gacagcgcga tcttcggcga cctgggttcg atcaacgcct
      121 tcctaccgat cggccagctg tcgctcgaca actacaccgg cgtcttcgac cgggtgccgt
      181 tcgccacgtt cctgctcaac tcggcgctga tctcggtcgt caccgtggtg ttcgggttga
      241 tcgtcaactc gatggccgcc ttcgcgttgt cgcggatgga gttcaagggg cagaaggtgc
      301 tgctcggcgt gatcctggcc accctcatcg tccccttcga gacgatggcg ctgccgctgc
      361 tgtggtgggt caaccagctg ccctacttcg acggttcgac cggatggctc gacacctatg
      421 aggtgcagat catcccgttc atcgccaacg cgttctcgat ctacctcttc taccagtact
      481 tcgactccat tcccaaagag ctggacgagg cggccaaggt cgacggcgcc ggctggttcc
      541 ggatctaccg cagcatcgtg atgccgctgt ccggaccggc ggtggcgacg gtggccatcc
      601 tcacctcctg ccggcttgga actcctacct gtggccgctg atggtgaccc agtccgaggc
      661 gatccgtccg gcgatggtcg gcatggacta cttcaagcag atgaccggcg cgaccggcac
      721 cgcgtgggga cagatcatgg cctacgcgtc gatgatcacc gtgcccgtgc tggtgctgtt
      781 catcgccttc cagcgggcgt tcatcaactc catcgccagc tccggcgtga agggctgagg
      841 ggcgatgttc gatccgcccg aagcgtgggt gtgggacttc tgggtcgtgg acgacggcga
      901 gcagtaccac ctgttctttc tgtatgcctc gcgggccctc aagaaccccg aggcccggca
      961 ctaccgtgcc tcggtgggcc acgcgatctc gtccgacctg acctgttgga cgcgggtcac
     1021 cgatgcgctg gtgcggtccg acgtgccggc gccggacgac ctggccacct ggaccggctc
     1081 ggtgatccag ggcccggacg gcttgtggta cctgttctac accggcgcaa ccgctgcccc
     1141 gaacggggcg aacgtgcaga ccatctgcta tgccacctcc ccggacctgt atgagtggac
     1201 gaaggcgccc gggccgattc tgcgagccga tccgcgctgg tacgagctgc tcagcgacgg
     1261 gcagtggcat gacgaggcgt tccgcgatcc cttcgtgttc gccgacccgg cgggtaacgg
     1321 ctggcacctg ttgatcacgg cgcgggccaa ccacgggccg tccgacgacc ggggggtggt
     1381 gggccacgcc tggtccccga acctgaccga ttgggagctg cgtccgccct tgtcgcggcc
     1441 gggtcagggg tacgggcagc tggaggtgtt cgagctcgtc cggctccagg gtcgtgacta
     1501 cctggtgttc aactgcctgg ccggcgacct gtcggcggcg catcgtgcat caggcaccac
     1561 cgggggagtc tggctggccg cggcggagtc gccgctgggg ccgtatgacc tggccggcgc
     1621 ccagctggtg accggagacg atctctacgt cggacggttc gtgactgctc ggtcaggtgc
     1681 gcccgtgttc ctggctttcc gcaacgccgg tccgggcggc accttcatgg gcggtatcac
     1741 cgacccgctg ccggtcagtg tggtgaacgg cagcgtttcg gtgggctgac ctctcgcggg
     1801 tcgagcgcgt cgagatcgcc ctcgac
//
LOCUS       DAOSUG010000542         1797 bp    DNA     linear   ENV 10-JUN-2024
DEFINITION  MAG TPA_asm: Micropruina sp. isolate AMR_MDS_2195
            NODE_26749_length_1797_cov_4.691734, whole genome shotgun sequence.
ACCESSION   DAOSUG010000542 DAOSUG010000000
VERSION     DAOSUG010000542.1
DBLINK      BioProject: PRJNA925477
            BioSample: SAMN32864262
            Sequence Read Archive: SRR4236673
KEYWORDS    WGS; ENV; Metagenome Assembled Genome; MAG; Third Party Data; TPA;
            TPA:assembly.
SOURCE      Micropruina sp. (wastewater metagenome)
  ORGANISM  Micropruina sp.
            Bacteria; Bacillati; Actinomycetota; Actinomycetes;
            Propionibacteriales; Nocardioidaceae; Micropruina.
REFERENCE   1  (bases 1 to 1797)
  AUTHORS   Abdulkadir,N., Saraiva,J.P., Zhang,J., Stolte,S., Gillor,O.,
            Harms,H. and Rocha,U.
  TITLE     Genome-centric analyses of 165 metagenomes show that mobile genetic
            elements are crucial for the transmission of antimicrobial
            resistance genes to pathogens in activated sludge and wastewater
  JOURNAL   Microbiol Spectr 12 (3), e0291823 (2024)
   PUBMED   38289113
REFERENCE   2  (bases 1 to 1797)
  AUTHORS   Abdulkadir,N.
  TITLE     Direct Submission
  JOURNAL   Submitted (24-MAR-2023) Environmental Microbiology, Helmholtz
            Center for Environmental Research -UFZ, Permoserstr 15, Leipzig
            04318, Germany
COMMENT     The annotation was added by the NCBI Prokaryotic Genome Annotation
            Pipeline (PGAP). Information about PGAP can be found here:
            https://www.ncbi.nlm.nih.gov/genome/annotation_prok/
            
            ##Genome-Assembly-Data-START##
            Assembly Method        :: SPAdes v. 3.11.1
            Genome Representation  :: Full
            Expected Final Version :: Yes
            Genome Coverage        :: Not Applicable
            Sequencing Technology  :: Illumina MiSeq
            ##Genome-Assembly-Data-END##
            
            ##Genome-Annotation-Data-START##
            Annotation Provider               :: NCBI
            Annotation Date                   :: 05/04/2023 04:57:10
            Annotation Pipeline               :: NCBI Prokaryotic Genome
                                                 Annotation Pipeline (PGAP)
            Annotation Method                 :: Best-placed reference protein
                                                 set; GeneMarkS-2+
            Annotation Software revision      :: 6.5
            Features Annotated                :: Gene; CDS; rRNA; tRNA; ncRNA
            Genes (total)                     :: 2,776
            CDSs (total)                      :: 2,750
            Genes (coding)                    :: 2,714
            CDSs (with protein)               :: 2,714
            Genes (RNA)                       :: 26
            tRNAs                             :: 24
            ncRNAs                            :: 2
            Pseudo Genes (total)              :: 36
            CDSs (without protein)            :: 36
            Pseudo Genes (ambiguous residues) :: 0 of 36
            Pseudo Genes (frameshifted)       :: 2 of 36
            Pseudo Genes (incomplete)         :: 32 of 36
            Pseudo Genes (internal stop)      :: 2 of 36
            ##Genome-Annotation-Data-END##
FEATURES             Location/Qualifiers
     source          1..1797
                     /organism="Micropruina sp."
                     /mol_type="genomic DNA"
                     /submitter_seqid="NODE_26749_length_1797_cov_4.691734"
                     /isolate="AMR_MDS_2195"
                     /isolation_source="Activated sludge of municipal
                     wastewater treatment plants"
                     /db_xref="taxon:2737536"
                     /environmental_sample
                     /geo_loc_name="Canada"
                     /lat_lon="49.25 N 123.1 W"
                     /collection_date="2015-06-16"
                     /metagenome_source="wastewater metagenome"
                     /note="metagenomic"
     gene            complement(<1..358)
                     /locus_tag="PLF56_13785"
     CDS             complement(<1..358)
                     /locus_tag="PLF56_13785"
                     /inference="COORDINATES: ab initio
                     prediction:GeneMarkS-2+"
                     /note="Derived by automated computational analysis using
                     gene prediction method: GeneMarkS-2+."
                     /codon_start=1
                     /transl_table=11
                     /product="metallopeptidase family protein"
                     /protein_id="HOQ54669.1"
                     /translation="MAHRRERHGRGLRGPLALPNPLTGAPVPLGSRPHRAEFFTACVS
                     AALGRVSAACPRALTGIDVGVEDVPVVAGPWSRDRVPLAAAIGPVPPAHGQVVLYRRP
                     LEHRARTRKGLRILVFR"
     gene            396..740
                     /locus_tag="PLF56_13790"
     CDS             396..740
                     /locus_tag="PLF56_13790"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_008600063.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="DUF3499 domain-containing protein"
                     /protein_id="HOQ54670.1"
                     /translation="MKFRSCSRSGCSARAIATLTYAYADSTAVLGPLALRAEPGCYDL
                     CAAHSASLSVPRGWEVIRLPDAEADAGPSTDDLLALARAVRVAGLGAEAEVRPEPAVT
                     RRKGHLAVIADS"
     gene            853..>1797
                     /gene="manB"
                     /locus_tag="PLF56_13795"
     CDS             853..>1797
                     /gene="manB"
                     /locus_tag="PLF56_13795"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_013864779.1"
                     /note="converts mannose-6-phosphate to
                     mannose-1-phosphate; the resulting product is then
                     converted to GDP-mannose by ManC which is then used in the
                     synthesis of mannose-containing glycoconjugates that are
                     important for mediating entry into host cells; Derived by
                     automated computational analysis using gene prediction
                     method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="phosphomannomutase/phosphoglucomutase"
                     /protein_id="HOQ54671.1"
                     /translation="MLTPSIFKANDIRGIVTGDDPEWNAEGARVLGGAFVHVLGLATG
                     AFVLGRDMRTSGPELAAAFIEGATAQGADVIDIGLASTDELWFASGMLDVPGVQLTAS
                     HNPAAYNGLKFCLAKAAPIEPALLKEIARVAMGPPIEPTVRPGECREVDLLADYAAHL
                     HALVPLEGLRRLTVVYDCGNGMAGHTAPAVLAPLNLELIGLFTDLDGSFPNHPPNPLE
                     PQNLVDAQAAVREHKADVGLVFDGDADRCFIIDERGEVVSPSLITALIAQQELDREPG
                     ATIVINTITSRAVNQVVKQAGGRLVISNVGHTSVKALMA"
BASE COUNT          267 a          654 c          623 g          253 t
ORIGIN      
        1 tgcggaacac cagaatgcgc aaccccttgc gggtgcgcgc ccggtgctcg agcggacgcc
       61 gatacagcac cacctgcccg tgtgccggcg gtaccggacc gatggccgcc gcgagcggca
      121 cccggtcgcg actccacggg ccggcgacca caggcacgtc ctcgacgccg acgtcgatgc
      181 cggtcagcgc gcgcgggcag gccgcggaca cccggcccaa ggctgcactg acgcaggcgg
      241 tgaagaactc cgcccggtgc gggcggctgc ccaatggcac cggggcgccg gtcagggggt
      301 tgggcagggc gaggggaccg cgcaggcccc gtccatgtcg ctccctgcgg tgtgccatgc
      361 gcccaaggtt aggccaagcc ggtaatgtca cgccggtgaa gttccggtcg tgttcgcgct
      421 cagggtgttc cgcccgcgcg atcgccactc tcacctacgc ctacgccgac tccaccgccg
      481 tgctcggccc gctggcgttg cgcgccgaac ccggctgcta cgacctgtgt gccgcccaca
      541 gcgcgagcct gtcggtgccg cgcggctggg aggtgattcg gctgcccgac gccgaagccg
      601 acgcgggccc gtccaccgac gacctgctgg cgttggcgcg ggccgtccgg gtggccggcc
      661 tgggcgccga ggccgaggtg cgtcccgaac cagcggtgac caggcgcaag ggtcatctcg
      721 cggtgatcgc cgacagctga actttcccgt cgggcgggta ctacgacacc acccggcccc
      781 gggattgcca gccaccagac ccggtgcctc tggccccggc gcccatcggt tgatccggga
      841 taggctgcgg acgtgctgac gccgtccatc ttcaaggcca acgacattcg tggcatcgtc
      901 accggtgatg atcccgagtg gaacgccgag ggggcgcggg ttctgggcgg cgcgttcgtc
      961 cacgtgctgg ggctggcgac cggcgcgttc gtgctcggcc gcgacatgcg caccagcggc
     1021 cccgaactcg ccgcggcttt tatcgagggc gccaccgccc agggtgccga cgtgatcgac
     1081 atcgggctgg ccagcaccga tgagctgtgg ttcgcctccg gcatgctgga cgtgcccggc
     1141 gtccagctca ccgcctcgca caatccggcc gcctacaacg ggctcaagtt ctgcctagcg
     1201 aaggcagcac cgatcgagcc ggcgctgctg aaagagatcg cccgggtggc gatgggcccg
     1261 ccgatcgagc ccaccgtgcg tccgggcgag tgccgcgagg tcgatctgct ggccgactac
     1321 gccgcgcacc tgcatgcgct ggtgccactg gagggcctgc ggcggctcac cgtggtctac
     1381 gactgcggca acggcatggc gggtcacacc gcgccggccg tgctggcacc gctcaacctc
     1441 gagctgatcg gcctgttcac cgacctcgac ggcagctttc ccaaccatcc gcccaacccg
     1501 ctcgagccgc agaacctggt ggacgcgcag gcagccgtcc gcgagcacaa ggcagacgtc
     1561 ggactggtgt tcgacggcga cgccgaccgc tgcttcatca tcgacgagcg cggcgaggtg
     1621 gtcagcccgt cgctgatcac cgccctgatc gcccagcagg agctcgatcg cgagcccggc
     1681 gccacgatcg tcatcaacac catcacctcg cgcgcggtca accaggtggt gaagcaggcc
     1741 ggcggacgac tcgtgatcag caatgtcggg cacacctcgg tcaaggcgct gatggcg
//
LOCUS       DAOSUG010000543         1786 bp    DNA     linear   ENV 10-JUN-2024
DEFINITION  MAG TPA_asm: Micropruina sp. isolate AMR_MDS_2195
            NODE_26970_length_1786_cov_3.484691, whole genome shotgun sequence.
ACCESSION   DAOSUG010000543 DAOSUG010000000
VERSION     DAOSUG010000543.1
DBLINK      BioProject: PRJNA925477
            BioSample: SAMN32864262
            Sequence Read Archive: SRR4236673
KEYWORDS    WGS; ENV; Metagenome Assembled Genome; MAG; Third Party Data; TPA;
            TPA:assembly.
SOURCE      Micropruina sp. (wastewater metagenome)
  ORGANISM  Micropruina sp.
            Bacteria; Bacillati; Actinomycetota; Actinomycetes;
            Propionibacteriales; Nocardioidaceae; Micropruina.
REFERENCE   1  (bases 1 to 1786)
  AUTHORS   Abdulkadir,N., Saraiva,J.P., Zhang,J., Stolte,S., Gillor,O.,
            Harms,H. and Rocha,U.
  TITLE     Genome-centric analyses of 165 metagenomes show that mobile genetic
            elements are crucial for the transmission of antimicrobial
            resistance genes to pathogens in activated sludge and wastewater
  JOURNAL   Microbiol Spectr 12 (3), e0291823 (2024)
   PUBMED   38289113
REFERENCE   2  (bases 1 to 1786)
  AUTHORS   Abdulkadir,N.
  TITLE     Direct Submission
  JOURNAL   Submitted (24-MAR-2023) Environmental Microbiology, Helmholtz
            Center for Environmental Research -UFZ, Permoserstr 15, Leipzig
            04318, Germany
COMMENT     The annotation was added by the NCBI Prokaryotic Genome Annotation
            Pipeline (PGAP). Information about PGAP can be found here:
            https://www.ncbi.nlm.nih.gov/genome/annotation_prok/
            
            ##Genome-Assembly-Data-START##
            Assembly Method        :: SPAdes v. 3.11.1
            Genome Representation  :: Full
            Expected Final Version :: Yes
            Genome Coverage        :: Not Applicable
            Sequencing Technology  :: Illumina MiSeq
            ##Genome-Assembly-Data-END##
            
            ##Genome-Annotation-Data-START##
            Annotation Provider               :: NCBI
            Annotation Date                   :: 05/04/2023 04:57:10
            Annotation Pipeline               :: NCBI Prokaryotic Genome
                                                 Annotation Pipeline (PGAP)
            Annotation Method                 :: Best-placed reference protein
                                                 set; GeneMarkS-2+
            Annotation Software revision      :: 6.5
            Features Annotated                :: Gene; CDS; rRNA; tRNA; ncRNA
            Genes (total)                     :: 2,776
            CDSs (total)                      :: 2,750
            Genes (coding)                    :: 2,714
            CDSs (with protein)               :: 2,714
            Genes (RNA)                       :: 26
            tRNAs                             :: 24
            ncRNAs                            :: 2
            Pseudo Genes (total)              :: 36
            CDSs (without protein)            :: 36
            Pseudo Genes (ambiguous residues) :: 0 of 36
            Pseudo Genes (frameshifted)       :: 2 of 36
            Pseudo Genes (incomplete)         :: 32 of 36
            Pseudo Genes (internal stop)      :: 2 of 36
            ##Genome-Annotation-Data-END##
FEATURES             Location/Qualifiers
     source          1..1786
                     /organism="Micropruina sp."
                     /mol_type="genomic DNA"
                     /submitter_seqid="NODE_26970_length_1786_cov_3.484691"
                     /isolate="AMR_MDS_2195"
                     /isolation_source="Activated sludge of municipal
                     wastewater treatment plants"
                     /db_xref="taxon:2737536"
                     /environmental_sample
                     /geo_loc_name="Canada"
                     /lat_lon="49.25 N 123.1 W"
                     /collection_date="2015-06-16"
                     /metagenome_source="wastewater metagenome"
                     /note="metagenomic"
     gene            371..1303
                     /locus_tag="PLF56_13800"
     CDS             371..1303
                     /locus_tag="PLF56_13800"
                     /inference="COORDINATES: protein motif:HMM:NF039614.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="arginine deiminase-related protein"
                     /protein_id="HOQ54672.1"
                     /translation="MNFRHVQAPTAVVMVRPHRFRVNGETLADNAFQAPSDVSLSAAD
                     LAVGARAEFDGCVEALTAAGVTVHVFDDLGQLDTPDSVFPNNWFSTHHGGRIAIYPMF
                     APNRRRERRADVIDLLKSTYRVQEVIDYSGLEYDNLFLEGTGAMVLDHLERVAYVANS
                     FRADPVILERFCTAFGYEPMAFPTAGPDGRPIYHTNVLMGIATEYALVCLAAISDERR
                     RAEVARRLADSGRSVLDLSFHQVAEFAGNAIELTGSDGRLLALSARAAAVLTDAQRDV
                     IEQSARLLPMAVPTIELAGGSVRCMIAGVHLTPR"
BASE COUNT          286 a          629 c          576 g          295 t
ORIGIN      
        1 gtggccgttg ctgcaacccc aagccggccc ggagtcgggc ccgaagggcc gcactccaag
       61 ccggcgtcga tgccgcacgc gtgtggacga atcgtccacg cacccgcgac gcggacgcgt
      121 gagtgggtac tactccgatg tccgcgctcc accgaggccg ccgcggacgc cctggacggt
      181 gcgtcggttg aaaccacagc cggcgcaccc cagtcttggt gcggccagcc cgaagcccga
      241 tccgggcctg taccgccttc gtcaaagggt gcggcgccgg gggtgctgag cctgcgccgc
      301 ggcgccgcct cgccgcctcg cctgacgcac cgcgtcacct agactgtcgc ggactcacca
      361 gggggacgct atgaactttc gtcacgtgca ggcgccgacg gcggtggtga tggtgcgtcc
      421 acaccgcttc agggtgaacg gcgaaaccct cgccgacaac gcctttcagg cgccgagtga
      481 cgtgtcgttg agcgccgccg acctggccgt cggtgcccga gccgagttcg acgggtgtgt
      541 cgaggcgctg accgctgccg gcgtcaccgt gcacgtgttc gacgacctcg gccaactgga
      601 cacccccgac tcggtctttc ccaacaactg gttcagcacc catcacggcg gccggatcgc
      661 catctacccg atgttcgcgc cgaaccgccg tcgcgaacgt cgcgccgatg tgatcgacct
      721 gctcaagagc acctatcggg tccaagaggt gatcgactat tccgggctcg agtacgacaa
      781 cctgttcctg gagggcaccg gtgcgatggt gctcgaccat ctggagcggg tggcgtacgt
      841 ggcgaactcg tttcgcgccg acccggtgat tctggagcga ttctgcaccg cgttcggcta
      901 cgagccgatg gcgtttccga ccgccgggcc ggacggtcgt ccgatctatc acaccaacgt
      961 gctgatgggc atcgccaccg aatacgcact ggtctgcctg gcagcgatca gcgacgagcg
     1021 gcggcgtgcc gaggtggcgc ggcggctggc cgacagcgga cgcagcgtgc tcgaccttag
     1081 ctttcaccag gtggccgagt tcgccggcaa cgccatcgag ctgaccggca gcgacgggcg
     1141 gttgctggcg ctgtctgcgc gggcggccgc cgtgctgacc gatgcccagc gcgacgtgat
     1201 cgagcagtcg gcgcggctgc tgcccatggc tgtgcccacc atcgaactgg ccggcggctc
     1261 ggtgcgctgc atgatcgcgg gggtgcacct caccccccgc tgagccgatc ccgccggccg
     1321 atcctggcaa gcctccttcg ccggtccagc ttgtcggcac cccatcactg atcctggtca
     1381 cgagagattt caccgttcgc agatataccg atattaccgc cagattctcc caaaagcggt
     1441 ttgcaacgat tcatcccaaa actggcggta gattcggtat atcagctgat gatgatttca
     1501 gcaggtcgtc ggttcgctct gagccagcac gctcgtgagc tcggtgagga ggccgtccgg
     1561 gtcgtcctgt agacgtggcc aggtgtagcg aagagcctga aatccggcgg ccagcgggcg
     1621 gtctccgcaa gcctgatccc cacactcgtc gcggccgccg cctggcccta cgcccagccc
     1681 agcgaagttc tcgccgccgc cggcaggcaa gacgccacgg tcgcagcgat gcaccgagag
     1741 tcaccacact cgtgcaacga accctcacct ccgcaggcct cctccc
//
LOCUS       DAOSUG010000544         1765 bp    DNA     linear   ENV 10-JUN-2024
DEFINITION  MAG TPA_asm: Micropruina sp. isolate AMR_MDS_2195
            NODE_27427_length_1765_cov_4.911696, whole genome shotgun sequence.
ACCESSION   DAOSUG010000544 DAOSUG010000000
VERSION     DAOSUG010000544.1
DBLINK      BioProject: PRJNA925477
            BioSample: SAMN32864262
            Sequence Read Archive: SRR4236673
KEYWORDS    WGS; ENV; Metagenome Assembled Genome; MAG; Third Party Data; TPA;
            TPA:assembly.
SOURCE      Micropruina sp. (wastewater metagenome)
  ORGANISM  Micropruina sp.
            Bacteria; Bacillati; Actinomycetota; Actinomycetes;
            Propionibacteriales; Nocardioidaceae; Micropruina.
REFERENCE   1  (bases 1 to 1765)
  AUTHORS   Abdulkadir,N., Saraiva,J.P., Zhang,J., Stolte,S., Gillor,O.,
            Harms,H. and Rocha,U.
  TITLE     Genome-centric analyses of 165 metagenomes show that mobile genetic
            elements are crucial for the transmission of antimicrobial
            resistance genes to pathogens in activated sludge and wastewater
  JOURNAL   Microbiol Spectr 12 (3), e0291823 (2024)
   PUBMED   38289113
REFERENCE   2  (bases 1 to 1765)
  AUTHORS   Abdulkadir,N.
  TITLE     Direct Submission
  JOURNAL   Submitted (24-MAR-2023) Environmental Microbiology, Helmholtz
            Center for Environmental Research -UFZ, Permoserstr 15, Leipzig
            04318, Germany
COMMENT     The annotation was added by the NCBI Prokaryotic Genome Annotation
            Pipeline (PGAP). Information about PGAP can be found here:
            https://www.ncbi.nlm.nih.gov/genome/annotation_prok/
            
            ##Genome-Assembly-Data-START##
            Assembly Method        :: SPAdes v. 3.11.1
            Genome Representation  :: Full
            Expected Final Version :: Yes
            Genome Coverage        :: Not Applicable
            Sequencing Technology  :: Illumina MiSeq
            ##Genome-Assembly-Data-END##
            
            ##Genome-Annotation-Data-START##
            Annotation Provider               :: NCBI
            Annotation Date                   :: 05/04/2023 04:57:10
            Annotation Pipeline               :: NCBI Prokaryotic Genome
                                                 Annotation Pipeline (PGAP)
            Annotation Method                 :: Best-placed reference protein
                                                 set; GeneMarkS-2+
            Annotation Software revision      :: 6.5
            Features Annotated                :: Gene; CDS; rRNA; tRNA; ncRNA
            Genes (total)                     :: 2,776
            CDSs (total)                      :: 2,750
            Genes (coding)                    :: 2,714
            CDSs (with protein)               :: 2,714
            Genes (RNA)                       :: 26
            tRNAs                             :: 24
            ncRNAs                            :: 2
            Pseudo Genes (total)              :: 36
            CDSs (without protein)            :: 36
            Pseudo Genes (ambiguous residues) :: 0 of 36
            Pseudo Genes (frameshifted)       :: 2 of 36
            Pseudo Genes (incomplete)         :: 32 of 36
            Pseudo Genes (internal stop)      :: 2 of 36
            ##Genome-Annotation-Data-END##
FEATURES             Location/Qualifiers
     source          1..1765
                     /organism="Micropruina sp."
                     /mol_type="genomic DNA"
                     /submitter_seqid="NODE_27427_length_1765_cov_4.911696"
                     /isolate="AMR_MDS_2195"
                     /isolation_source="Activated sludge of municipal
                     wastewater treatment plants"
                     /db_xref="taxon:2737536"
                     /environmental_sample
                     /geo_loc_name="Canada"
                     /lat_lon="49.25 N 123.1 W"
                     /collection_date="2015-06-16"
                     /metagenome_source="wastewater metagenome"
                     /note="metagenomic"
     gene            147..1610
                     /locus_tag="PLF56_13805"
     CDS             147..1610
                     /locus_tag="PLF56_13805"
                     /inference="COORDINATES: protein motif:HMM:NF024491.2"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="phospholipase D-like domain-containing protein"
                     /protein_id="HOQ54673.1"
                     /translation="MTILTAITLSAALATGSASALSFDTDAESVIEVPTTSGCPTLTD
                     EPRAYEAWFNVDDMEVRGYYDPLNQVPWDFPKKIAQILCGARQGAEIKIGMYFIRAIG
                     TMTQPGLKAGADPAGSLGSRPESDPEVIYDALEYLVKKRGVKVGLVLDAGSITSASAK
                     SLINRRLLTIAGISGIPSTAGGKTDGIEWCTNGCFNTNSRSTYPYAINHEKYVTISDT
                     IWDGASGSARPASSAKPAVISSSGNWARSQIRNYMQELTVVYGDRELFNQFSMRYDGM
                     TYCANTKCAKNTGFPSGLKKNLYLDRKIWVDPTIHGTDSGRGTFVTFSPQPSTVVDSY
                     IAAFDNVDCTVDKRIRIAMFKLTDSKATTMAKALSRLKSRGCDVKMLLTQQGGATSIS
                     KGVIKMLNKAKISYKCTAVAMHTKLILIGPVTGNNGRALHGTANMSTAGLRYSEEHTI
                     TFDARRAAPQYQEDLRRVYGTYLAGWYELSQGSKACK"
BASE COUNT          358 a          585 c          528 g          294 t
ORIGIN      
        1 cgaccggtgc cgacaggggc tcacaactgg atcaagtatg ctacggcgag ttggcgggga
       61 tttcgtgctg cggcgcgccg aggcaaatcg atgtgaaagc gggagcggta atgggtttgt
      121 tcgaacggtc tgttcgaggc agattattga caattctgac cgccatcaca ctctctgcgg
      181 cactcgccac cgggtcggcc tcggccctgt ctttcgacac cgacgccgag tccgtgatcg
      241 aagttccgac cacctccggg tgtcccaccc tcactgatga gccgcgggcc tacgaggcgt
      301 ggttcaacgt ggacgacatg gaggtccgcg gctactacga cccgctgaac caggtgccgt
      361 gggacttccc gaagaagatc gcgcagattc tgtgtggcgc ccgccagggt gccgagatca
      421 agatcggcat gtacttcatc cgcgccatcg gcaccatgac gcagccgggt ctgaaggccg
      481 gcgctgaccc cgccggctcg ttgggcagcc ggcccgagag cgacccggag gtcatctacg
      541 acgcgctgga atacctggtc aagaagcgtg gcgtcaaggt cggactggtg ctcgatgccg
      601 gttcgatcac ttcggcctcg gccaagtcac tgatcaaccg acgccttctc acgatcgcgg
      661 gcatcagcgg cattcccagc acggccggtg gcaagaccga cggcatcgag tggtgcacga
      721 acggttgctt caacaccaac tcgcgttcga cctatcccta cgcgatcaac catgagaagt
      781 acgtcacgat cagtgacacc atctgggacg gcgcctcggg ttcggctcgt ccggcctcct
      841 cggccaagcc ggccgtgatc tcgtcctcgg gcaactgggc gcgcagccag attcgcaact
      901 acatgcagga actgaccgtc gtctacggcg atcgagaact gttcaaccag ttcagcatgc
      961 gctatgacgg catgacgtac tgcgccaaca ccaaatgtgc gaagaacacc ggctttccgt
     1021 ccgggttgaa gaagaacctg tatctcgacc gcaagatctg ggtcgacccc acgattcacg
     1081 gcaccgactc aggacgtggc acgttcgtca ccttcagccc ccaaccctca accgtggtcg
     1141 acagctacat cgccgcgttc gacaacgtcg actgcaccgt cgacaagcgc atccgcatcg
     1201 ccatgttcaa gctgaccgac agcaaggcca ccacgatggc gaaggccctg tcgcggctga
     1261 agagccgtgg ctgcgacgtg aagatgctgc tgacccagca gggtggcgcc acgtcgatct
     1321 cgaagggcgt gatcaaaatg ctcaacaagg ccaagatctc gtacaagtgc accgccgtgg
     1381 ccatgcacac caagttgatt ctgatcggtc cggtcaccgg caacaacgga cgcgccctgc
     1441 acggcaccgc caacatgtcg acagccggcc tgcgctacag cgaagagcac accatcacct
     1501 tcgacgcccg ccgggcagca ccgcagtacc aggaagatct gaggcgtgtc tacggcacct
     1561 acctcgccgg ctggtacgag ttgtcgcagg gctcgaaggc gtgcaagtga tgaggacccc
     1621 caccatgacc aagtcccccg ccagcaccgc accagccggg gtcaaggtac ccaaactgct
     1681 gatcgccgtc atcgccgtgg tggtgctgct ggtcggcggc tccatcgccg gcaccgcgct
     1741 gggcgcagcc ggcccgaagc cgacg
//
LOCUS       DAOSUG010000545         1717 bp    DNA     linear   ENV 10-JUN-2024
DEFINITION  MAG TPA_asm: Micropruina sp. isolate AMR_MDS_2195
            NODE_28493_length_1717_cov_4.520457, whole genome shotgun sequence.
ACCESSION   DAOSUG010000545 DAOSUG010000000
VERSION     DAOSUG010000545.1
DBLINK      BioProject: PRJNA925477
            BioSample: SAMN32864262
            Sequence Read Archive: SRR4236673
KEYWORDS    WGS; ENV; Metagenome Assembled Genome; MAG; Third Party Data; TPA;
            TPA:assembly.
SOURCE      Micropruina sp. (wastewater metagenome)
  ORGANISM  Micropruina sp.
            Bacteria; Bacillati; Actinomycetota; Actinomycetes;
            Propionibacteriales; Nocardioidaceae; Micropruina.
REFERENCE   1  (bases 1 to 1717)
  AUTHORS   Abdulkadir,N., Saraiva,J.P., Zhang,J., Stolte,S., Gillor,O.,
            Harms,H. and Rocha,U.
  TITLE     Genome-centric analyses of 165 metagenomes show that mobile genetic
            elements are crucial for the transmission of antimicrobial
            resistance genes to pathogens in activated sludge and wastewater
  JOURNAL   Microbiol Spectr 12 (3), e0291823 (2024)
   PUBMED   38289113
REFERENCE   2  (bases 1 to 1717)
  AUTHORS   Abdulkadir,N.
  TITLE     Direct Submission
  JOURNAL   Submitted (24-MAR-2023) Environmental Microbiology, Helmholtz
            Center for Environmental Research -UFZ, Permoserstr 15, Leipzig
            04318, Germany
COMMENT     The annotation was added by the NCBI Prokaryotic Genome Annotation
            Pipeline (PGAP). Information about PGAP can be found here:
            https://www.ncbi.nlm.nih.gov/genome/annotation_prok/
            
            ##Genome-Assembly-Data-START##
            Assembly Method        :: SPAdes v. 3.11.1
            Genome Representation  :: Full
            Expected Final Version :: Yes
            Genome Coverage        :: Not Applicable
            Sequencing Technology  :: Illumina MiSeq
            ##Genome-Assembly-Data-END##
            
            ##Genome-Annotation-Data-START##
            Annotation Provider               :: NCBI
            Annotation Date                   :: 05/04/2023 04:57:10
            Annotation Pipeline               :: NCBI Prokaryotic Genome
                                                 Annotation Pipeline (PGAP)
            Annotation Method                 :: Best-placed reference protein
                                                 set; GeneMarkS-2+
            Annotation Software revision      :: 6.5
            Features Annotated                :: Gene; CDS; rRNA; tRNA; ncRNA
            Genes (total)                     :: 2,776
            CDSs (total)                      :: 2,750
            Genes (coding)                    :: 2,714
            CDSs (with protein)               :: 2,714
            Genes (RNA)                       :: 26
            tRNAs                             :: 24
            ncRNAs                            :: 2
            Pseudo Genes (total)              :: 36
            CDSs (without protein)            :: 36
            Pseudo Genes (ambiguous residues) :: 0 of 36
            Pseudo Genes (frameshifted)       :: 2 of 36
            Pseudo Genes (incomplete)         :: 32 of 36
            Pseudo Genes (internal stop)      :: 2 of 36
            ##Genome-Annotation-Data-END##
FEATURES             Location/Qualifiers
     source          1..1717
                     /organism="Micropruina sp."
                     /mol_type="genomic DNA"
                     /submitter_seqid="NODE_28493_length_1717_cov_4.520457"
                     /isolate="AMR_MDS_2195"
                     /isolation_source="Activated sludge of municipal
                     wastewater treatment plants"
                     /db_xref="taxon:2737536"
                     /environmental_sample
                     /geo_loc_name="Canada"
                     /lat_lon="49.25 N 123.1 W"
                     /collection_date="2015-06-16"
                     /metagenome_source="wastewater metagenome"
                     /note="metagenomic"
     gene            <1..423
                     /locus_tag="PLF56_13810"
     CDS             <1..423
                     /locus_tag="PLF56_13810"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_012919256.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="adenosylhomocysteinase"
                     /protein_id="HOQ54674.1"
                     /translation="AIVGNIGHFDNEIDMAGLETYPGIERTVIKPQVDRWTFPDGHSI
                     LVLSEGRLLNLGNATGHPSFVMSNSFTNQVLAQIELYTKPEEYPIAVYTLPKALDEEV
                     ARLHLDALGAKLTTLSSDQAEYLGVAVDGPFKADSYRY"
     gene            663..926
                     /gene="rpsO"
                     /locus_tag="PLF56_13815"
     CDS             663..926
                     /gene="rpsO"
                     /locus_tag="PLF56_13815"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_002548327.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_component: GO:0000314 - organellar small ribosomal
                     subunit [Evidence IEA];
                     GO_component: GO:0022627 - cytosolic small ribosomal
                     subunit [Evidence IEA];
                     GO_function: GO:0003735 - structural constituent of
                     ribosome [Evidence IEA];
                     GO_process: GO:0006412 - translation [Evidence IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="30S ribosomal protein S15"
                     /protein_id="HOQ54675.1"
                     /translation="MDADLKKKTIEEYATKPGDTGSPDVQIALLTRRIAHLTEHLKVH
                     KGDHHSRRGLLLLVGQRRRLLNYVAKQDVEHYRELIARLGLRR"
     gene            1206..>1717
                     /locus_tag="PLF56_13820"
     CDS             1206..>1717
                     /locus_tag="PLF56_13820"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_002516891.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="polyribonucleotide nucleotidyltransferase"
                     /protein_id="HOQ54676.1"
                     /translation="MEGPDLQYTEAVIDNGSHGKHVVRFESGLLARQANGSAAVYLDG
                     DTMLLSATTASNNPRDGIDFFPLTVDVEERMYAAGRIPGSFFRREGRPGEAAILACRL
                     TDRPLRPCFVKGLRNEVQVVITVMALNPEVLYDVLAINAGSASTTLAGLPFTGPVGGV
                     RVALIGNDWVG"
BASE COUNT          302 a          615 c          534 g          266 t
ORIGIN      
        1 gcgatcgtcg gcaacatcgg ccacttcgac aacgagatcg acatggccgg gctcgagacc
       61 taccccggca tcgaacgcac cgtgatcaag ccgcaggtcg accggtggac cttccccgac
      121 gggcattcga tcctggtgct ctccgaaggg cggctgctca acctgggcaa cgccaccggc
      181 cacccgtcgt tcgtgatgag caactcgttc accaaccagg tgctggctca gatcgagctg
      241 tacaccaagc ccgaggagta cccgatcgcg gtgtacaccc tgcccaaggc gctggacgaa
      301 gaggtggccc ggctgcacct ggacgcgctg ggcgcgaagc tgaccacgct gtcgtccgat
      361 caggccgagt acctgggtgt cgcggtcgac ggccccttca aggcggactc gtaccgctac
      421 tgaccccccg ccgagcgccc gcctaagtat ctctggcctg cgaagcttcg caggccagag
      481 atacttaggc gggggttcgc ggggacgatt tccggctcgc cggactgctg cggtagagtt
      541 catggttggc gtcagagcgc ccgcggactc gcaagagcat cccatcgaaa tccaagcgga
      601 aatcgtgcct tcggcaggga tgcgccgcgc aacgggaaac cgagaggaga gcagcagcct
      661 tcatggacgc tgacctgaag aagaagacca tcgaagagta cgcgaccaag ccgggtgaca
      721 ccgggtcgcc cgacgtgcag atcgccctgc tcacccgtcg gatcgctcac ctgaccgagc
      781 acctcaaggt gcacaagggc gaccaccaca gccgccgtgg cctgttgctg ctggtcggcc
      841 agcgccgccg gctgctgaac tacgtcgcca agcaggacgt cgagcactac cgcgaactga
      901 tcgcgcggct cggcctgcgt cgctgacttc gacaggggag tggcctcggc cgctcccctg
      961 acgactttcg gtcggccgag caccaccatt gcgatggtcg ggccggaccc gaccgcaccc
     1021 gacaactgaa tacccaacca ccgtccaccg cgcattcgtc acgtgaatcc tcggtctccg
     1081 gtagtggctt tcgggtcttt caccccgcgg gcctcgatcg aagaccgggt tcaacagccc
     1141 caaggttgca tagccgaaca gtggccgtgt cgccgcagcg gtgggcacac ccgaaaggag
     1201 tccccgtgga gggacctgat ctgcagtaca ccgaagccgt catcgacaac ggctcccacg
     1261 gcaagcacgt cgtccggttc gagtcgggcc tgctggcccg gcaggccaac ggctcggccg
     1321 ccgtctacct ggacggcgac accatgctgc tgtccgccac caccgcgtcc aacaacccgc
     1381 gcgacggcat cgacttcttc ccgctcaccg tcgacgtcga agagcgcatg tacgccgccg
     1441 gccgtattcc cggctcgttc ttccgtcgcg aaggccgtcc gggcgaggcc gcgatcctgg
     1501 cctgccggct caccgaccgt ccgctgcggc cctgcttcgt caagggcctg cgcaacgagg
     1561 tccaggtcgt catcaccgtg atggcgctca accccgaggt gctctacgac gtgctggcga
     1621 tcaacgccgg ctcggcgtcg accacgctcg ccggcctccc gttcaccggc ccggtcggcg
     1681 gcgtccgcgt cgccctgatc ggcaacgact gggtcgg
//
LOCUS       DAOSUG010000546         1710 bp    DNA     linear   ENV 10-JUN-2024
DEFINITION  MAG TPA_asm: Micropruina sp. isolate AMR_MDS_2195
            NODE_28650_length_1710_cov_4.124471, whole genome shotgun sequence.
ACCESSION   DAOSUG010000546 DAOSUG010000000
VERSION     DAOSUG010000546.1
DBLINK      BioProject: PRJNA925477
            BioSample: SAMN32864262
            Sequence Read Archive: SRR4236673
KEYWORDS    WGS; ENV; Metagenome Assembled Genome; MAG; Third Party Data; TPA;
            TPA:assembly.
SOURCE      Micropruina sp. (wastewater metagenome)
  ORGANISM  Micropruina sp.
            Bacteria; Bacillati; Actinomycetota; Actinomycetes;
            Propionibacteriales; Nocardioidaceae; Micropruina.
REFERENCE   1  (bases 1 to 1710)
  AUTHORS   Abdulkadir,N., Saraiva,J.P., Zhang,J., Stolte,S., Gillor,O.,
            Harms,H. and Rocha,U.
  TITLE     Genome-centric analyses of 165 metagenomes show that mobile genetic
            elements are crucial for the transmission of antimicrobial
            resistance genes to pathogens in activated sludge and wastewater
  JOURNAL   Microbiol Spectr 12 (3), e0291823 (2024)
   PUBMED   38289113
REFERENCE   2  (bases 1 to 1710)
  AUTHORS   Abdulkadir,N.
  TITLE     Direct Submission
  JOURNAL   Submitted (24-MAR-2023) Environmental Microbiology, Helmholtz
            Center for Environmental Research -UFZ, Permoserstr 15, Leipzig
            04318, Germany
COMMENT     The annotation was added by the NCBI Prokaryotic Genome Annotation
            Pipeline (PGAP). Information about PGAP can be found here:
            https://www.ncbi.nlm.nih.gov/genome/annotation_prok/
            
            ##Genome-Assembly-Data-START##
            Assembly Method        :: SPAdes v. 3.11.1
            Genome Representation  :: Full
            Expected Final Version :: Yes
            Genome Coverage        :: Not Applicable
            Sequencing Technology  :: Illumina MiSeq
            ##Genome-Assembly-Data-END##
            
            ##Genome-Annotation-Data-START##
            Annotation Provider               :: NCBI
            Annotation Date                   :: 05/04/2023 04:57:10
            Annotation Pipeline               :: NCBI Prokaryotic Genome
                                                 Annotation Pipeline (PGAP)
            Annotation Method                 :: Best-placed reference protein
                                                 set; GeneMarkS-2+
            Annotation Software revision      :: 6.5
            Features Annotated                :: Gene; CDS; rRNA; tRNA; ncRNA
            Genes (total)                     :: 2,776
            CDSs (total)                      :: 2,750
            Genes (coding)                    :: 2,714
            CDSs (with protein)               :: 2,714
            Genes (RNA)                       :: 26
            tRNAs                             :: 24
            ncRNAs                            :: 2
            Pseudo Genes (total)              :: 36
            CDSs (without protein)            :: 36
            Pseudo Genes (ambiguous residues) :: 0 of 36
            Pseudo Genes (frameshifted)       :: 2 of 36
            Pseudo Genes (incomplete)         :: 32 of 36
            Pseudo Genes (internal stop)      :: 2 of 36
            ##Genome-Annotation-Data-END##
FEATURES             Location/Qualifiers
     source          1..1710
                     /organism="Micropruina sp."
                     /mol_type="genomic DNA"
                     /submitter_seqid="NODE_28650_length_1710_cov_4.124471"
                     /isolate="AMR_MDS_2195"
                     /isolation_source="Activated sludge of municipal
                     wastewater treatment plants"
                     /db_xref="taxon:2737536"
                     /environmental_sample
                     /geo_loc_name="Canada"
                     /lat_lon="49.25 N 123.1 W"
                     /collection_date="2015-06-16"
                     /metagenome_source="wastewater metagenome"
                     /note="metagenomic"
     gene            265..>1710
                     /locus_tag="PLF56_13825"
     CDS             265..>1710
                     /locus_tag="PLF56_13825"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_012920922.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="ABC transporter ATP-binding protein"
                     /protein_id="HOQ54677.1"
                     /translation="MSSLTRDSSVREHELAPGTRRRVLSYAAAYRKQIVIFLALTMVD
                     SLLVVASPLILQRLIDDGVVPGDAGLVIRLAVLVAVIAIADAALTLVERWYSSRIGEG
                     LIYDLRTQVFSHVLRQPIAFFTRTQTGALVTRLNSDVIGAQQAFTSVLQNLVGNAISL
                     VVIVAAMASLSWQLTIGALLLVPLFLVPARLIGRRLAGLTREQMQQNAQMGAQMSERF
                     NVAGALLVKLFGDPRREDQEYSGHAAGVRDVGVKLAVNRSVFFVALALVASLATAMVY
                     GFGGVMAVNGLLTVGTLLALAALLARLYAPLTALSNVRVDVMTALVSFERVFEVLDLK
                     PLVAEQPNAVDIPDQPVRVEVDHVTFGYPSADEISLASLEGAATGDRRGSGDILHDLT
                     FTVPAGSMVALVGPSGAGKTTITSLVARLYDPTEGAIRVNGVDLRDATLSSLHDRVGV
                     VTQEAHLFHDTIRMNLLYAKPSATDAELEAAL"
BASE COUNT          236 a          558 c          601 g          315 t
ORIGIN      
        1 tgcggtggcc agtccacgtg gcagcgtcgc ggccggcggg aatcagtgtc acccactccg
       61 ttcactgccg gggggcgggt cgatgcggtg gccagtccac gtggcagcgt cgcggccggc
      121 gggaatcagt gtcacccaat ccgttcactt gccggggttg ggtcgatgcg gcggccagtc
      181 cacgtgacag cgttgcaacc ggcgggaatc agtgtcggtg gcgtgtggtt gagttcaacc
      241 gtgagcatga gtggttggca ggtcatgagt tcgctgaccc gtgattcgtc ggtgcgcgag
      301 cacgagcttg cgcccggaac gcggcgccgg gtgctcagct atgccgccgc ctaccgcaag
      361 cagatcgtga tctttcttgc gctcaccatg gtcgactcgc tgctggtggt cgcctcgccg
      421 ctgattctgc agcggctgat cgacgacggt gtggtgccgg gcgatgccgg cttggtgatt
      481 cggctggcgg tgctcgtggc ggtgatcgcg atcgccgacg cagcgctcac cctggtcgag
      541 cggtggtaca gctcacgcat cggtgagggc ctgatctacg accttcgcac ccaggttttc
      601 tcgcatgtgc tgcgccagcc gatcgcgttc ttcacccgca cccagaccgg tgccctggtc
      661 acccggctga acagcgacgt gatcggcgcc cagcaggcgt tcacctcggt gctgcagaac
      721 ctggtcggta atgcgatcag cctggtggtg atcgtggcgg ccatggcgtc gctgtcgtgg
      781 cagctcacca tcggggcatt gctgttggtg ccgctgtttc tggtgccggc ccgcctgatc
      841 ggccgccggc tggctggtct gacccgtgag cagatgcagc agaacgccca gatgggcgcc
      901 cagatgagcg agcggttcaa cgttgccggc gccctgctgg tgaagctgtt cggtgacccg
      961 cggcgtgaag accaggagta ctccgggcac gcggcagggg tgcgtgacgt cggggtgaag
     1021 ctggcggtca accggtcggt gttcttcgtc gcgttggccc tggtggcatc gctggccacg
     1081 gccatggtct acggcttcgg cggcgtgatg gccgtgaacg gcctgctcac ggtcggcacc
     1141 ctgctcgcat tggccgccct gctggcccgg ctgtatgcgc cgctcacggc actgtcgaac
     1201 gtgcgggtcg acgtgatgac cgcactggtg tcgttcgaac gggtcttcga ggtgctcgac
     1261 ctgaagccgt tggttgccga gcagcccaac gccgtcgaca ttcccgacca gccggtgcgg
     1321 gtcgaggtcg accacgtcac gttcggctac ccgtccgcgg acgagatctc gctcgcctcg
     1381 ctcgaaggcg cggccaccgg cgaccggcgg ggcagcggcg acattctgca cgacctgacc
     1441 ttcaccgtgc cggccggctc gatggtggcc ctggtcggcc ccagtggcgc gggcaagacc
     1501 accatcacct cgctggtggc ccggctgtac gaccccaccg aaggcgcgat ccgggtgaac
     1561 ggcgtcgacc tgcgcgacgc caccctgagc tcgctgcacg accgggtcgg cgtggtcacg
     1621 caagaggcgc acctgtttca cgacaccatc cgcatgaacc tgttgtacgc caagccgtcc
     1681 gccaccgacg ctgaactcga ggcggcgctg
//
LOCUS       DAOSUG010000547         1602 bp    DNA     linear   ENV 10-JUN-2024
DEFINITION  MAG TPA_asm: Micropruina sp. isolate AMR_MDS_2195
            NODE_31453_length_1602_cov_4.850032, whole genome shotgun sequence.
ACCESSION   DAOSUG010000547 DAOSUG010000000
VERSION     DAOSUG010000547.1
DBLINK      BioProject: PRJNA925477
            BioSample: SAMN32864262
            Sequence Read Archive: SRR4236673
KEYWORDS    WGS; ENV; Metagenome Assembled Genome; MAG; Third Party Data; TPA;
            TPA:assembly.
SOURCE      Micropruina sp. (wastewater metagenome)
  ORGANISM  Micropruina sp.
            Bacteria; Bacillati; Actinomycetota; Actinomycetes;
            Propionibacteriales; Nocardioidaceae; Micropruina.
REFERENCE   1  (bases 1 to 1602)
  AUTHORS   Abdulkadir,N., Saraiva,J.P., Zhang,J., Stolte,S., Gillor,O.,
            Harms,H. and Rocha,U.
  TITLE     Genome-centric analyses of 165 metagenomes show that mobile genetic
            elements are crucial for the transmission of antimicrobial
            resistance genes to pathogens in activated sludge and wastewater
  JOURNAL   Microbiol Spectr 12 (3), e0291823 (2024)
   PUBMED   38289113
REFERENCE   2  (bases 1 to 1602)
  AUTHORS   Abdulkadir,N.
  TITLE     Direct Submission
  JOURNAL   Submitted (24-MAR-2023) Environmental Microbiology, Helmholtz
            Center for Environmental Research -UFZ, Permoserstr 15, Leipzig
            04318, Germany
COMMENT     The annotation was added by the NCBI Prokaryotic Genome Annotation
            Pipeline (PGAP). Information about PGAP can be found here:
            https://www.ncbi.nlm.nih.gov/genome/annotation_prok/
            
            ##Genome-Assembly-Data-START##
            Assembly Method        :: SPAdes v. 3.11.1
            Genome Representation  :: Full
            Expected Final Version :: Yes
            Genome Coverage        :: Not Applicable
            Sequencing Technology  :: Illumina MiSeq
            ##Genome-Assembly-Data-END##
            
            ##Genome-Annotation-Data-START##
            Annotation Provider               :: NCBI
            Annotation Date                   :: 05/04/2023 04:57:10
            Annotation Pipeline               :: NCBI Prokaryotic Genome
                                                 Annotation Pipeline (PGAP)
            Annotation Method                 :: Best-placed reference protein
                                                 set; GeneMarkS-2+
            Annotation Software revision      :: 6.5
            Features Annotated                :: Gene; CDS; rRNA; tRNA; ncRNA
            Genes (total)                     :: 2,776
            CDSs (total)                      :: 2,750
            Genes (coding)                    :: 2,714
            CDSs (with protein)               :: 2,714
            Genes (RNA)                       :: 26
            tRNAs                             :: 24
            ncRNAs                            :: 2
            Pseudo Genes (total)              :: 36
            CDSs (without protein)            :: 36
            Pseudo Genes (ambiguous residues) :: 0 of 36
            Pseudo Genes (frameshifted)       :: 2 of 36
            Pseudo Genes (incomplete)         :: 32 of 36
            Pseudo Genes (internal stop)      :: 2 of 36
            ##Genome-Annotation-Data-END##
FEATURES             Location/Qualifiers
     source          1..1602
                     /organism="Micropruina sp."
                     /mol_type="genomic DNA"
                     /submitter_seqid="NODE_31453_length_1602_cov_4.850032"
                     /isolate="AMR_MDS_2195"
                     /isolation_source="Activated sludge of municipal
                     wastewater treatment plants"
                     /db_xref="taxon:2737536"
                     /environmental_sample
                     /geo_loc_name="Canada"
                     /lat_lon="49.25 N 123.1 W"
                     /collection_date="2015-06-16"
                     /metagenome_source="wastewater metagenome"
                     /note="metagenomic"
     gene            complement(<1..497)
                     /locus_tag="PLF56_13830"
     CDS             complement(<1..497)
                     /locus_tag="PLF56_13830"
                     /inference="COORDINATES: protein motif:HMM:NF024811.2"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="HAD hydrolase-like protein"
                     /protein_id="HOQ54678.1"
                     /translation="MSERALILDCDGVLADTEKDGHLVAFNQVFEEAGYPFRWSEDEY
                     SELLKVGGGKERMKAYLAQHPELDLPGSDDLNALLAEVHKRKSADYQQIVNSGQIPGR
                     PGVKRLIEDALDHGWRVAIASTSAVPSVEAVAVAVLGQATRDRLFGIYGGDIVKAKKP
                     APDIYL"
     gene            complement(494..1375)
                     /locus_tag="PLF56_13835"
     CDS             complement(494..1375)
                     /locus_tag="PLF56_13835"
                     /inference="COORDINATES: protein motif:HMM:NF013296.2"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="class II fructose-bisphosphate aldolase"
                     /protein_id="HOQ54679.1"
                     /translation="MPYVNTLDLVTAARGQGYAVPAINIVDPLTIKAVVEAADSIGSP
                     VILQTSVKTVKSAGAGLLHYAAREAADAASVGVSLHLDHCPDREVITLAIKQGWSSVL
                     FDASDRDLDQARKETAEVCQEAHAAGASVESEIENIIGVEDGVGSDVAKHSYTVEQLI
                     EVALETGTDLMAPQLGTAHGMYSAQPVLLPERVRQIRALSDIPVVLHGGTGLSNAQFD
                     EFIDAGVTKINISTALKQAYMKSALEYLKQCEATNKWEPTKLFDPMIAASKALLIEFA
                     GIFHSAGRDGAAKDGAR"
BASE COUNT          243 a          547 c          514 g          298 t
ORIGIN      
        1 aggtagatgt cgggagccgg cttcttggcc ttgacgatgt cgccgccgta gatgccgaac
       61 agccggtcgc gggtggcctg gcccagcacc gccaccgcga cggcctcgac actgggtacc
      121 gccgaggtgg acgcgatcgc cacccgccag ccgtgatcca gtgcgtcctc gatcagtcgt
      181 ttcacgcccg gtcggccggg aatctgaccc gagttgacga tctgctggta gtcggcgctc
      241 ttgcgcttgt gcacctcggc gagcagcgcg ttcagatcat ccgaaccggg caggtccagc
      301 tcggggtgct gcgccaggta cgccttcatg cgctccttgc cgccacccac cttgagcagc
      361 tcggagtact cgtcctcgga ccagcggaac gggtagcccg cctcttcgaa gacctggttg
      421 aacgcgacca ggtggccgtc cttctcggtg tcggccagca ccccgtcgca gtcgaggatc
      481 agcgcgcgct cgctcatcgg gctccgtcct tggccgcccc gtcacgaccc gccgagtgga
      541 agatgcccgc gaactcgatc agcagcgcct tcgaggcggc gatcatcggg tcgaacaact
      601 tggtcggctc ccacttgttg gtcgcctcgc actgcttcag gtattcgagc gccgacttca
      661 tgtaggcctg cttgagcgcg gtcgagatgt tgatcttggt caccccggcg tcgatgaact
      721 cgtcgaactg ggcgttgctc agcccggtgc cgccgtgcag caccaccgga atgtcgctca
      781 gtgcccggat ctgccgcacg cgctcgggca gcagcacagg ctgcgcggaa tacatgccgt
      841 gcgctgtgcc cagctgcggc gccatcaggt cggtgccggt ttcgagggcc acctcgatca
      901 gctgctcgac ggtgtaggag tgcttggcga cgtcagagcc gacgccgtcc tcgacgccga
      961 tgatgttctc gatctcgctc tccacgctcg ccccggcggc gtgcgcctct tggcacacct
     1021 cggcggtttc tttgcgggcc tggtcgaggt cgcgatcgga cgcgtcgaac agcaccgagc
     1081 tccagccctg cttgatcgcc agcgtgatca cctcgcgatc ggggcagtgg tccaggtgaa
     1141 gcgacacccc gacggacgcc gcgtcggcgg cctcgcgtgc cgcgtagtgc agcaggccgg
     1201 cgcccgcgga cttcacggtc ttcacagagg tctgcagaat caccggcgat ccgatcgagt
     1261 cggcggcctc gaccaccgct ttgatggtca gcggatcgac gatgttgatc gcgggcaccg
     1321 cgtagccctg acccctggcc gcagtgacca gatcgagggt gttgacgtat ggcatggggc
     1381 tccttcggct gactcgacgt tgggtcggcg aactcgtatg tacaactata gacaacttgg
     1441 acgggcgtgg ctaccgcgac ttcgtgccgg ccacctggaa gaccaccttg cgaccttggc
     1501 cgaccaggtc cagtccatcg ttccagcgct ccagcggcaa tacctcgctg atgaacgatt
     1561 cggcatcgat gcgtccgtcg gccatcagcc gcagcgccgt tc
//
LOCUS       DAOSUG010000548         1593 bp    DNA     linear   ENV 10-JUN-2024
DEFINITION  MAG TPA_asm: Micropruina sp. isolate AMR_MDS_2195
            NODE_31707_length_1593_cov_4.297139, whole genome shotgun sequence.
ACCESSION   DAOSUG010000548 DAOSUG010000000
VERSION     DAOSUG010000548.1
DBLINK      BioProject: PRJNA925477
            BioSample: SAMN32864262
            Sequence Read Archive: SRR4236673
KEYWORDS    WGS; ENV; Metagenome Assembled Genome; MAG; Third Party Data; TPA;
            TPA:assembly.
SOURCE      Micropruina sp. (wastewater metagenome)
  ORGANISM  Micropruina sp.
            Bacteria; Bacillati; Actinomycetota; Actinomycetes;
            Propionibacteriales; Nocardioidaceae; Micropruina.
REFERENCE   1  (bases 1 to 1593)
  AUTHORS   Abdulkadir,N., Saraiva,J.P., Zhang,J., Stolte,S., Gillor,O.,
            Harms,H. and Rocha,U.
  TITLE     Genome-centric analyses of 165 metagenomes show that mobile genetic
            elements are crucial for the transmission of antimicrobial
            resistance genes to pathogens in activated sludge and wastewater
  JOURNAL   Microbiol Spectr 12 (3), e0291823 (2024)
   PUBMED   38289113
REFERENCE   2  (bases 1 to 1593)
  AUTHORS   Abdulkadir,N.
  TITLE     Direct Submission
  JOURNAL   Submitted (24-MAR-2023) Environmental Microbiology, Helmholtz
            Center for Environmental Research -UFZ, Permoserstr 15, Leipzig
            04318, Germany
COMMENT     The annotation was added by the NCBI Prokaryotic Genome Annotation
            Pipeline (PGAP). Information about PGAP can be found here:
            https://www.ncbi.nlm.nih.gov/genome/annotation_prok/
            
            ##Genome-Assembly-Data-START##
            Assembly Method        :: SPAdes v. 3.11.1
            Genome Representation  :: Full
            Expected Final Version :: Yes
            Genome Coverage        :: Not Applicable
            Sequencing Technology  :: Illumina MiSeq
            ##Genome-Assembly-Data-END##
            
            ##Genome-Annotation-Data-START##
            Annotation Provider               :: NCBI
            Annotation Date                   :: 05/04/2023 04:57:10
            Annotation Pipeline               :: NCBI Prokaryotic Genome
                                                 Annotation Pipeline (PGAP)
            Annotation Method                 :: Best-placed reference protein
                                                 set; GeneMarkS-2+
            Annotation Software revision      :: 6.5
            Features Annotated                :: Gene; CDS; rRNA; tRNA; ncRNA
            Genes (total)                     :: 2,776
            CDSs (total)                      :: 2,750
            Genes (coding)                    :: 2,714
            CDSs (with protein)               :: 2,714
            Genes (RNA)                       :: 26
            tRNAs                             :: 24
            ncRNAs                            :: 2
            Pseudo Genes (total)              :: 36
            CDSs (without protein)            :: 36
            Pseudo Genes (ambiguous residues) :: 0 of 36
            Pseudo Genes (frameshifted)       :: 2 of 36
            Pseudo Genes (incomplete)         :: 32 of 36
            Pseudo Genes (internal stop)      :: 2 of 36
            ##Genome-Annotation-Data-END##
FEATURES             Location/Qualifiers
     source          1..1593
                     /organism="Micropruina sp."
                     /mol_type="genomic DNA"
                     /submitter_seqid="NODE_31707_length_1593_cov_4.297139"
                     /isolate="AMR_MDS_2195"
                     /isolation_source="Activated sludge of municipal
                     wastewater treatment plants"
                     /db_xref="taxon:2737536"
                     /environmental_sample
                     /geo_loc_name="Canada"
                     /lat_lon="49.25 N 123.1 W"
                     /collection_date="2015-06-16"
                     /metagenome_source="wastewater metagenome"
                     /note="metagenomic"
     gene            complement(<1..257)
                     /locus_tag="PLF56_13840"
     CDS             complement(<1..257)
                     /locus_tag="PLF56_13840"
                     /inference="COORDINATES: ab initio
                     prediction:GeneMarkS-2+"
                     /note="Derived by automated computational analysis using
                     gene prediction method: GeneMarkS-2+."
                     /codon_start=1
                     /transl_table=11
                     /product="hypothetical protein"
                     /protein_id="HOQ54680.1"
                     /translation="MGTDLRSVERVTGRRNWGTVMVQGLQSGLSTGLMVTFILWFLQP
                     QTNPLVLLPGALLIGILIGIAFAAIGYAASRGKRDFNSISQT"
     gene            complement(300..1427)
                     /locus_tag="PLF56_13845"
     CDS             complement(300..1427)
                     /locus_tag="PLF56_13845"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_013861089.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_function: GO:0000976 - transcription cis-regulatory
                     region binding [Evidence IEA];
                     GO_function: GO:0003700 - DNA-binding transcription factor
                     activity [Evidence IEA];
                     GO_function: GO:0043565 - sequence-specific DNA binding
                     [Evidence IEA];
                     GO_process: GO:0006351 - DNA-templated transcription
                     [Evidence IEA];
                     GO_process: GO:0006355 - regulation of DNA-templated
                     transcription [Evidence IEA];
                     GO_process: GO:0045892 - negative regulation of
                     DNA-templated transcription [Evidence IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="LacI family DNA-binding transcriptional
                     regulator"
                     /protein_id="HOQ54681.1"
                     /translation="MIENPGAEGDDQPGRAVRGAGRGVRLLDVANAAGVSVATASRSL
                     RGVAKVSPATRERVLDAARELSYGVTPRLFEADPAQPGAPVVAVILPFFSRWYFSTAT
                     AAAFEHLRANGYDVLLYHLGTAEVRDQFFETLPLAGRVQGVLSLSMPLGEHHTLALRA
                     LGLPLVSIGSHIDGWPSVGIDDVAAGTLAVNHLLNQGHQRIGLIAGAVDDARFDFTAS
                     VARRLGYEQALNGAGIEFDPALVVEGPHGIEGGAQAMTELLGRPHLPTAVLAEFDELA
                     IGALWALRRAGLQVPRDVSVIGIDDMDMARFVDLTTVAQDVVAQGRYAAVQLIRLLTG
                     EAGPEDAVQRLEPTRLVLRDSTAPPHRGRAAGASLLNANEP"
BASE COUNT          262 a          572 c          531 g          228 t
ORIGIN      
        1 gtctggctga tcgagttgaa gtcacgcttg ccgcgtgagg cggcgtaccc gatcgccgcg
       61 aaggcgatgc cgatcagaat gccgatcagc aacgcaccgg gcagcagcac cagcgggttg
      121 gtctgcggct gcaggaacca cagaatgaac gtgaccatca gaccggtcga cagtccgctc
      181 tgcaggccct gcaccatgac ggttccccag ttgcgccgtc cggtcacccg ctcgaccgac
      241 ctcaggtcgg tgcccacgat cgcgcagccc cgatcggggc ccgtgagggc gtcgaccggt
      301 cagggttcgt tcgcattcag cagcgacgca cccgcggccc gaccccggtg cggcggtgcc
      361 gtgctgtcgc gcaacaccag ccgggtgggc tcgagccgct gcacggcgtc ctcgggcccg
      421 gcctcgccgg tgagcagcct gatcagctga acggctgcgt accggccctg ggcgaccacg
      481 tcctgtgcga ccgtcgtgag atcgacgaag cgggccatgt ccatgtcgtc gatgccgatc
      541 accgacacgt cgcgcggcac ctgcagcccc gcgcggcgca gcgcccagag cgcaccgatg
      601 gccaactcgt cgaactcggc gagcaccgcg gtgggcaggt gcggtcggcc cagcagctcg
      661 gtcatcgcct gggcgccgcc ctcgatgccg tgcggcccct cgaccaccag agccgggtcg
      721 aactcgatgc cggcgccgtt caacgcctgc tcgtaaccca accgccgggc caccgacgcg
      781 gtgaaatcga agcgggcgtc gtcgaccgcg ccggcgatca gcccgatgcg ctggtgcccc
      841 tgattgagca ggtgattcac cgccaaggtg cccgcagcca cgtcgtcgat gcccaccgat
      901 ggccacccgt cgatgtgcga accgatgctc accagcggca gtccgagtgc ccgcaacgcc
      961 agcgtgtggt gttcgccaag cggcatcgac agcgagagaa cgccctgcac ccggccggcg
     1021 agcggcagcg tttcgaagaa ctggtcgcgc acctcggcgg tgcccaggtg gtagagcagc
     1081 acgtcgtagc cgttggcgcg caggtgctca aacgccgccg ctgtggcggt cgagaagtac
     1141 cagcggctga agaacggcaa gatcaccgcc accacggggg cgccgggctg ggcggggtca
     1201 gcctcgaaca gccgtggcgt gacgccgtac gacagctcgc gggccgcgtc caacaccctt
     1261 tcgcgggtgg ccggcgacac cttggcgacc ccgcgcagcg agcgtgaggc ggtggccacc
     1321 gacacgccgg cggcattcgc cacgtccagc agccggacgc cccgacccgc cccgcggacg
     1381 gcgcgcccgg gctggtcgtc accctcggcg ccggggttct cgatcacggc atcagcctat
     1441 tcaggttcgg gcacgggcgg cctcgatcat cacgtttcag ggccttcatc ttgccacttg
     1501 aatgcaaaca atgacagatg gacctacatt tccaccacct cgggccaacg gtttacccga
     1561 ctgtggcgtt gtgccagact tggcgttgca ata
//
LOCUS       DAOSUG010000549         1592 bp    DNA     linear   ENV 10-JUN-2024
DEFINITION  MAG TPA_asm: Micropruina sp. isolate AMR_MDS_2195
            NODE_31744_length_1592_cov_4.044242, whole genome shotgun sequence.
ACCESSION   DAOSUG010000549 DAOSUG010000000
VERSION     DAOSUG010000549.1
DBLINK      BioProject: PRJNA925477
            BioSample: SAMN32864262
            Sequence Read Archive: SRR4236673
KEYWORDS    WGS; ENV; Metagenome Assembled Genome; MAG; Third Party Data; TPA;
            TPA:assembly.
SOURCE      Micropruina sp. (wastewater metagenome)
  ORGANISM  Micropruina sp.
            Bacteria; Bacillati; Actinomycetota; Actinomycetes;
            Propionibacteriales; Nocardioidaceae; Micropruina.
REFERENCE   1  (bases 1 to 1592)
  AUTHORS   Abdulkadir,N., Saraiva,J.P., Zhang,J., Stolte,S., Gillor,O.,
            Harms,H. and Rocha,U.
  TITLE     Genome-centric analyses of 165 metagenomes show that mobile genetic
            elements are crucial for the transmission of antimicrobial
            resistance genes to pathogens in activated sludge and wastewater
  JOURNAL   Microbiol Spectr 12 (3), e0291823 (2024)
   PUBMED   38289113
REFERENCE   2  (bases 1 to 1592)
  AUTHORS   Abdulkadir,N.
  TITLE     Direct Submission
  JOURNAL   Submitted (24-MAR-2023) Environmental Microbiology, Helmholtz
            Center for Environmental Research -UFZ, Permoserstr 15, Leipzig
            04318, Germany
COMMENT     The annotation was added by the NCBI Prokaryotic Genome Annotation
            Pipeline (PGAP). Information about PGAP can be found here:
            https://www.ncbi.nlm.nih.gov/genome/annotation_prok/
            
            ##Genome-Assembly-Data-START##
            Assembly Method        :: SPAdes v. 3.11.1
            Genome Representation  :: Full
            Expected Final Version :: Yes
            Genome Coverage        :: Not Applicable
            Sequencing Technology  :: Illumina MiSeq
            ##Genome-Assembly-Data-END##
            
            ##Genome-Annotation-Data-START##
            Annotation Provider               :: NCBI
            Annotation Date                   :: 05/04/2023 04:57:10
            Annotation Pipeline               :: NCBI Prokaryotic Genome
                                                 Annotation Pipeline (PGAP)
            Annotation Method                 :: Best-placed reference protein
                                                 set; GeneMarkS-2+
            Annotation Software revision      :: 6.5
            Features Annotated                :: Gene; CDS; rRNA; tRNA; ncRNA
            Genes (total)                     :: 2,776
            CDSs (total)                      :: 2,750
            Genes (coding)                    :: 2,714
            CDSs (with protein)               :: 2,714
            Genes (RNA)                       :: 26
            tRNAs                             :: 24
            ncRNAs                            :: 2
            Pseudo Genes (total)              :: 36
            CDSs (without protein)            :: 36
            Pseudo Genes (ambiguous residues) :: 0 of 36
            Pseudo Genes (frameshifted)       :: 2 of 36
            Pseudo Genes (incomplete)         :: 32 of 36
            Pseudo Genes (internal stop)      :: 2 of 36
            ##Genome-Annotation-Data-END##
FEATURES             Location/Qualifiers
     source          1..1592
                     /organism="Micropruina sp."
                     /mol_type="genomic DNA"
                     /submitter_seqid="NODE_31744_length_1592_cov_4.044242"
                     /isolate="AMR_MDS_2195"
                     /isolation_source="Activated sludge of municipal
                     wastewater treatment plants"
                     /db_xref="taxon:2737536"
                     /environmental_sample
                     /geo_loc_name="Canada"
                     /lat_lon="49.25 N 123.1 W"
                     /collection_date="2015-06-16"
                     /metagenome_source="wastewater metagenome"
                     /note="metagenomic"
     gene            <1..549
                     /locus_tag="PLF56_13850"
     CDS             <1..549
                     /locus_tag="PLF56_13850"
                     /inference="COORDINATES: ab initio
                     prediction:GeneMarkS-2+"
                     /note="Derived by automated computational analysis using
                     gene prediction method: GeneMarkS-2+."
                     /codon_start=1
                     /transl_table=11
                     /product="hypothetical protein"
                     /protein_id="HOQ54682.1"
                     /translation="PPSSEDALLADLAEAGGLEAVLSGRSQLPGGDAAIATSGVSSKN
                     VAAAATWVVVCTPKANNPHVSRQTGPRSLPGVIFKTTVTCVGSGPHPAAVTVRVRGLL
                     NFMPAENERDTDGTWRGVLRSDYTQSILVNGKKYTFYTPMSEYRGAVGRGFYRGTGTT
                     QIIVPAGLTSGSNTSGVFWLTL"
     gene            complement(538..819)
                     /locus_tag="PLF56_13855"
     CDS             complement(538..819)
                     /locus_tag="PLF56_13855"
                     /inference="COORDINATES: ab initio
                     prediction:GeneMarkS-2+"
                     /note="Derived by automated computational analysis using
                     gene prediction method: GeneMarkS-2+."
                     /codon_start=1
                     /transl_table=11
                     /product="hypothetical protein"
                     /protein_id="HOQ54683.1"
                     /translation="MEPKPVDERDTRWEADKAPLRIFIHSPGHTVGAYDTECSLAAAE
                     AWATTHDEAVGYQVALRVDDARDGKGLIWLIDRAPTEGAQLGRERKSEG"
     gene            complement(918..>1592)
                     /locus_tag="PLF56_13860"
     CDS             complement(918..>1592)
                     /locus_tag="PLF56_13860"
                     /inference="COORDINATES: protein motif:HMM:NF013753.2"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="transposase"
                     /protein_id="HOQ54684.1"
                     /translation="LRAHPRQRQACLDDRGEDFRKNIDVAALDLFGGYKCAIDDQLQD
                     ATAVLDAFHVVKLGTQVVDEVRRRVQQDTTGHRGRKGDPLFGIQTILRAGAENLTDKQ
                     QARLVRAIESDEAHEEVFLAWQCAQQLRAAYRVRDLAEGRRLAEKVLASFHTCPIPEV
                     ARLGRTLRRWHCAFLAYFTTGRANNGGTEAMNGIIELHRRLARGYRNRENYRLRMLLA
                     AGGLTR"
BASE COUNT          246 a          514 c          545 g          287 t
ORIGIN      
        1 ccgccaagct cggaagacgc gctcctcgca gatctcgctg aagccggagg actggaagca
       61 gtactgtccg ggcgcagcca acttcctggt ggggacgccg ctatcgcgac atcaggggtg
      121 tcgtcgaaga acgtcgcggc cgccgcgacc tgggttgtcg tgtgcacgcc gaaggccaac
      181 aacccgcacg tgtcccggca gaccggtcct cgctccctcc cgggggttat cttcaagacg
      241 accgtgacct gcgtggggtc tggaccgcac ccggcagctg tcaccgtccg tgttcggggg
      301 ctgctgaact tcatgcccgc agagaatgag cgggataccg atggcacgtg gcgcggggtt
      361 ctgaggagcg actacacaca gtccatcctc gtcaacggca agaagtacac gttctatacc
      421 cccatgtcgg agtaccgcgg agcagtgggg cgcgggttct atcgaggtac tggaaccacc
      481 cagataatcg ttccggctgg actcacatcg gggtcgaaca cgtctggggt cttctggcta
      541 accctctgac tttcgctcgc gccccagctg cgcaccctcg gttggggcgc gatcgatcag
      601 ccagatgagg cccttgccat cgcgcgcgtc gtcgacacgc agagccactt ggtagccgac
      661 cgcttcgtcg tgcgtcgtag cccaggcctc cgcggctgcc agtgagcact ccgtgtcgta
      721 ggcaccgacg gtgtggcccg gcgaatggat gaagatgcgc aggggggcct tgtctgcctc
      781 ccacctcgtg tctcgctcgt cgactggttt cggctccata gcctgaacgt agccgctgta
      841 gaggccgcgc ggccatagtc ggcctcggcc cccgcacatc taccaggtat ggcggctctt
      901 cgccgatccg gtagggatca cctcgtgagt ccacctgcgg cgagcagcat ccgtagccgg
      961 tagttctcgc ggttgcggta accgcgggcg aggcgccggt ggagctcgat gatgccgttc
     1021 atggcttcag tgccaccgtt gtttgctcgg cccgtcgtga agtaggcgag gaacgcgcag
     1081 tgccagcggc gcagggtccg tccgaggcgg gcgacttccg ggatcgggca ggtgtggaac
     1141 gacgcgagga ccttctcggc caagcgacgg ccctcggcca gatctcggac ccggtaggcg
     1201 gcccgcagct gctgggcgca ctgccaggcg aggaacactt cctcgtgggc ttcatcggac
     1261 tcgatcgctc ggaccaggcg ggcctgctgc ttgtcggtga ggttctcggc gccggcgcgc
     1321 aggatggtct ggatgccgaa cagcgggtcg cccttgcgac cgcggtggcc ggttgtgtcc
     1381 tgctggaccc ggcgacgcac ctcgtcgacg acctgcgtgc cgagcttgac gacgtggaac
     1441 gcgtccagga ccgcggtggc gtcttgcagc tggtcgtcga tggcgcactt gtagccgccg
     1501 aacagatcca acgccgcgac gtcgatgttc ttgcggaagt cctcgccgcg gtcatcgagg
     1561 catgcctggc gctgtcgggg atgagcgcgg ag
//
LOCUS       DAOSUG010000550         1536 bp    DNA     linear   ENV 10-JUN-2024
DEFINITION  MAG TPA_asm: Micropruina sp. isolate AMR_MDS_2195
            NODE_33330_length_1536_cov_4.350439, whole genome shotgun sequence.
ACCESSION   DAOSUG010000550 DAOSUG010000000
VERSION     DAOSUG010000550.1
DBLINK      BioProject: PRJNA925477
            BioSample: SAMN32864262
            Sequence Read Archive: SRR4236673
KEYWORDS    WGS; ENV; Metagenome Assembled Genome; MAG; Third Party Data; TPA;
            TPA:assembly.
SOURCE      Micropruina sp. (wastewater metagenome)
  ORGANISM  Micropruina sp.
            Bacteria; Bacillati; Actinomycetota; Actinomycetes;
            Propionibacteriales; Nocardioidaceae; Micropruina.
REFERENCE   1  (bases 1 to 1536)
  AUTHORS   Abdulkadir,N., Saraiva,J.P., Zhang,J., Stolte,S., Gillor,O.,
            Harms,H. and Rocha,U.
  TITLE     Genome-centric analyses of 165 metagenomes show that mobile genetic
            elements are crucial for the transmission of antimicrobial
            resistance genes to pathogens in activated sludge and wastewater
  JOURNAL   Microbiol Spectr 12 (3), e0291823 (2024)
   PUBMED   38289113
REFERENCE   2  (bases 1 to 1536)
  AUTHORS   Abdulkadir,N.
  TITLE     Direct Submission
  JOURNAL   Submitted (24-MAR-2023) Environmental Microbiology, Helmholtz
            Center for Environmental Research -UFZ, Permoserstr 15, Leipzig
            04318, Germany
COMMENT     The annotation was added by the NCBI Prokaryotic Genome Annotation
            Pipeline (PGAP). Information about PGAP can be found here:
            https://www.ncbi.nlm.nih.gov/genome/annotation_prok/
            
            ##Genome-Assembly-Data-START##
            Assembly Method        :: SPAdes v. 3.11.1
            Genome Representation  :: Full
            Expected Final Version :: Yes
            Genome Coverage        :: Not Applicable
            Sequencing Technology  :: Illumina MiSeq
            ##Genome-Assembly-Data-END##
            
            ##Genome-Annotation-Data-START##
            Annotation Provider               :: NCBI
            Annotation Date                   :: 05/04/2023 04:57:10
            Annotation Pipeline               :: NCBI Prokaryotic Genome
                                                 Annotation Pipeline (PGAP)
            Annotation Method                 :: Best-placed reference protein
                                                 set; GeneMarkS-2+
            Annotation Software revision      :: 6.5
            Features Annotated                :: Gene; CDS; rRNA; tRNA; ncRNA
            Genes (total)                     :: 2,776
            CDSs (total)                      :: 2,750
            Genes (coding)                    :: 2,714
            CDSs (with protein)               :: 2,714
            Genes (RNA)                       :: 26
            tRNAs                             :: 24
            ncRNAs                            :: 2
            Pseudo Genes (total)              :: 36
            CDSs (without protein)            :: 36
            Pseudo Genes (ambiguous residues) :: 0 of 36
            Pseudo Genes (frameshifted)       :: 2 of 36
            Pseudo Genes (incomplete)         :: 32 of 36
            Pseudo Genes (internal stop)      :: 2 of 36
            ##Genome-Annotation-Data-END##
FEATURES             Location/Qualifiers
     source          1..1536
                     /organism="Micropruina sp."
                     /mol_type="genomic DNA"
                     /submitter_seqid="NODE_33330_length_1536_cov_4.350439"
                     /isolate="AMR_MDS_2195"
                     /isolation_source="Activated sludge of municipal
                     wastewater treatment plants"
                     /db_xref="taxon:2737536"
                     /environmental_sample
                     /geo_loc_name="Canada"
                     /lat_lon="49.25 N 123.1 W"
                     /collection_date="2015-06-16"
                     /metagenome_source="wastewater metagenome"
                     /note="metagenomic"
     gene            <1..1288
                     /locus_tag="PLF56_13865"
     CDS             <1..1288
                     /locus_tag="PLF56_13865"
                     /EC_number="4.1.1.31"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_007079540.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_function: GO:0008964 - phosphoenolpyruvate carboxylase
                     activity [Evidence IEA];
                     GO_process: GO:0006099 - tricarboxylic acid cycle
                     [Evidence IEA];
                     GO_process: GO:0015977 - carbon fixation [Evidence IEA]"
                     /codon_start=2
                     /transl_table=11
                     /product="phosphoenolpyruvate carboxylase"
                     /protein_id="HOQ54685.1"
                     /translation="GRSVIESYIVSMTRGPDDLLAAVLLARQARLVDVTEGFATIGFV
                     PLLETVAELRTAGEILERLLSDPSYRQIVRLRGDLQEVMLGYSDSNKDAGIATSQWEI
                     HRAQRVLRDVAAKHGIQLRLFHGRGGTVGRGGGPTHEAILAQPYGSLRGQIKVTEQGE
                     VISDKYTLPALARENLELTVAAVLEASTLHLTSRQSPEQLAEWDATMQDLSDAAFVAY
                     RGLVEDPDLPAYFWQSTPVDQLGALKLGSRPSKRPDSGSGLEGLRAIPWVFGWMQSRQ
                     VVPGWFGVGTGLEATVAAGHGDELAQMYDEWHFFRTFISNVEMMLTKTRLEVARHYVN
                     SLVEAQLQHVFHTIEAEYERTVRAVLAITGESELLESQPLLKRTLSVRDAYLDPLSYL
                     QVAMLSRLRAGDRDPQLERALLLAVNGVAAGLRNTG"
BASE COUNT          245 a          546 c          517 g          228 t
ORIGIN      
        1 cggccgcagc gtcatcgaga gctacatcgt gtcgatgacc agaggcccgg acgacctgct
       61 ggccgcggtg ctgctggccc gccaggcgcg cctggtcgac gtgaccgagg gcttcgccac
      121 gatcggcttc gtgccgctgc tcgagaccgt tgccgagctg cgcaccgccg gcgagattct
      181 cgagcggctg ctcagcgacc ccagctaccg ccagatcgtg cggctgcgcg gcgacctgca
      241 agaggtgatg ctcggctact ccgacagcaa caaggacgcc ggcatcgcca ccagccagtg
      301 ggagatccac cgggcccaac gggtgctgcg tgacgtggcc gccaagcacg gcattcagtt
      361 gcggctgttc cacggccggg gcggcacggt cggacgcggc ggtggcccca cccacgaggc
      421 gatcctggcc cagccgtacg gctcgttgcg cggccagatc aaggtcaccg aacagggtga
      481 ggtgatcagc gacaagtaca cgctgccggc cctggccagg gagaacctcg aactcaccgt
      541 ggcagccgtg ttggaggcca gcacgctgca cctgacctcg cggcagtccc ccgaacagct
      601 cgccgagtgg gacgccacca tgcaggacct gtcggacgcc gccttcgtcg cctaccgcgg
      661 cctggtggag gatccggacc tgccggccta cttctggcag tcgacgccgg tcgatcagct
      721 gggcgcgctc aagctgggct cacgcccgtc caagcggccc gacagcggca gcggcctgga
      781 ggggctgcgg gcgattccgt gggtgttcgg ctggatgcag tcgcggcagg tggtgcccgg
      841 ctggttcggc gtgggcaccg ggctggaggc caccgtcgcg gccggccacg gcgacgagct
      901 ggcgcagatg tacgacgagt ggcacttctt tcgcacgttc atcagcaacg tcgagatgat
      961 gctgaccaag acccgcctcg aggtggcccg ccactacgtg aacagcctgg tggaagcgca
     1021 gttgcagcac gtctttcaca ccatcgaggc cgagtacgag cgcaccgtgc gggccgtgct
     1081 ggcgatcacc ggcgagtcgg agttgctcga gagccagcct ttgctcaagc gcacgctgag
     1141 cgtgcgggac gcctacctcg acccgctcag ctacctgcag gtggccatgc tgtcgcggct
     1201 gcgcgcgggt gatcgcgacc cgcaactcga gcgggcgctg ttgctggccg tcaacggcgt
     1261 ggccgccggc ctgcgcaaca ccgggtaggg gcctttcgcg cttgtcgaga cccctgcatc
     1321 ggacatccag gcgaacgact ccgtcatccc ggttgaacga ctccgtcatc ccggttgaac
     1381 gactccgtca tcccggcgaa cgccgggatc tccccaagca cccctgcccc agacgcgggg
     1441 ccccctgcaa acccctgcat ccgtcatccc ggctgaacga ctccgtcatc ccggcgaacg
     1501 ccgggatctc cccaagcacc cctgccctga cgaggg
//
LOCUS       DAOSUG010000551         1534 bp    DNA     linear   ENV 10-JUN-2024
DEFINITION  MAG TPA_asm: Micropruina sp. isolate AMR_MDS_2195
            NODE_33398_length_1534_cov_4.851927, whole genome shotgun sequence.
ACCESSION   DAOSUG010000551 DAOSUG010000000
VERSION     DAOSUG010000551.1
DBLINK      BioProject: PRJNA925477
            BioSample: SAMN32864262
            Sequence Read Archive: SRR4236673
KEYWORDS    WGS; ENV; Metagenome Assembled Genome; MAG; Third Party Data; TPA;
            TPA:assembly.
SOURCE      Micropruina sp. (wastewater metagenome)
  ORGANISM  Micropruina sp.
            Bacteria; Bacillati; Actinomycetota; Actinomycetes;
            Propionibacteriales; Nocardioidaceae; Micropruina.
REFERENCE   1  (bases 1 to 1534)
  AUTHORS   Abdulkadir,N., Saraiva,J.P., Zhang,J., Stolte,S., Gillor,O.,
            Harms,H. and Rocha,U.
  TITLE     Genome-centric analyses of 165 metagenomes show that mobile genetic
            elements are crucial for the transmission of antimicrobial
            resistance genes to pathogens in activated sludge and wastewater
  JOURNAL   Microbiol Spectr 12 (3), e0291823 (2024)
   PUBMED   38289113
REFERENCE   2  (bases 1 to 1534)
  AUTHORS   Abdulkadir,N.
  TITLE     Direct Submission
  JOURNAL   Submitted (24-MAR-2023) Environmental Microbiology, Helmholtz
            Center for Environmental Research -UFZ, Permoserstr 15, Leipzig
            04318, Germany
COMMENT     The annotation was added by the NCBI Prokaryotic Genome Annotation
            Pipeline (PGAP). Information about PGAP can be found here:
            https://www.ncbi.nlm.nih.gov/genome/annotation_prok/
            
            ##Genome-Assembly-Data-START##
            Assembly Method        :: SPAdes v. 3.11.1
            Genome Representation  :: Full
            Expected Final Version :: Yes
            Genome Coverage        :: Not Applicable
            Sequencing Technology  :: Illumina MiSeq
            ##Genome-Assembly-Data-END##
            
            ##Genome-Annotation-Data-START##
            Annotation Provider               :: NCBI
            Annotation Date                   :: 05/04/2023 04:57:10
            Annotation Pipeline               :: NCBI Prokaryotic Genome
                                                 Annotation Pipeline (PGAP)
            Annotation Method                 :: Best-placed reference protein
                                                 set; GeneMarkS-2+
            Annotation Software revision      :: 6.5
            Features Annotated                :: Gene; CDS; rRNA; tRNA; ncRNA
            Genes (total)                     :: 2,776
            CDSs (total)                      :: 2,750
            Genes (coding)                    :: 2,714
            CDSs (with protein)               :: 2,714
            Genes (RNA)                       :: 26
            tRNAs                             :: 24
            ncRNAs                            :: 2
            Pseudo Genes (total)              :: 36
            CDSs (without protein)            :: 36
            Pseudo Genes (ambiguous residues) :: 0 of 36
            Pseudo Genes (frameshifted)       :: 2 of 36
            Pseudo Genes (incomplete)         :: 32 of 36
            Pseudo Genes (internal stop)      :: 2 of 36
            ##Genome-Annotation-Data-END##
FEATURES             Location/Qualifiers
     source          1..1534
                     /organism="Micropruina sp."
                     /mol_type="genomic DNA"
                     /submitter_seqid="NODE_33398_length_1534_cov_4.851927"
                     /isolate="AMR_MDS_2195"
                     /isolation_source="Activated sludge of municipal
                     wastewater treatment plants"
                     /db_xref="taxon:2737536"
                     /environmental_sample
                     /geo_loc_name="Canada"
                     /lat_lon="49.25 N 123.1 W"
                     /collection_date="2015-06-16"
                     /metagenome_source="wastewater metagenome"
                     /note="metagenomic"
     gene            complement(<1..1274)
                     /locus_tag="PLF56_13870"
     CDS             complement(<1..1274)
                     /locus_tag="PLF56_13870"
                     /inference="COORDINATES: protein motif:HMM:NF033550.5"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_function: GO:0004803 - transposase activity [Evidence
                     IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="ISL3 family transposase"
                     /protein_id="HOQ54686.1"
                     /translation="MTKPTVTCCATPDAYCSRIDTLVDLDGVHVVAVVRSGAEVTLTI
                     ETPRERQGCRRCGVVALSHGRRTRLLRDVPCAGSAVLLRWRQRTWACPDDACPAGTFT
                     EELSSLASPRAVLTTRAIWWAIGQLRREHATVEGLSRQLGVTWNTLWRVVEPRLAELA
                     GDESRFDGVTTLGVDEHVWHHTPHRARDKGPTMLTGMVDLSRDQDGRTRARLLDLVPG
                     RSGKVFGDWLAGRGGEFCAGVKVATLDPFRGYANAIDDHLDEATPALDAFHVVKLGGK
                     ALDEVRRRVQQTTLGRRGHAQDPLYRIRNILHRGQEHLTVRQGERLDECLSEGDPDDE
                     VQVAWRCYQQLRTTYRAKDAAEGKALAEKILETFHTCPIPEIARLGKTLRRWREQFLG
                     YFTTDRANNGGTEAINGIIELHRRIARGYRNR"
BASE COUNT          238 a          517 c          512 g          267 t
ORIGIN      
        1 tcgcggttgc ggtatccgcg tgcgatgcgt ctgtggagtt cgatgattcc gttgatggct
       61 tcggtgccgc cgttgttggc tcggtcggtg gtgaagtagc cgaggaactg ctcgcgccag
      121 cgtcgcaggg tcttgcccag gcgggcgatc tcggggatgg ggcaggtgtg gaacgtctcg
      181 aggatcttct cggccagcgc tttgccctcg gcggcgtcct tggcccggta ggtcgttcgc
      241 agctgttggt agcaccgcca agcgacctga acctcgtcgt cggggtcacc ctcgctcagg
      301 cactcatcga ggcgttcgcc ctggcgcacg gtgaggtgct cttggccgcg gtggaggatg
      361 ttgcggatcc ggtagagcgg gtcctgagcg tgcccgcgcc ggcccagggt ggtctgttgg
      421 actcggcggc ggacctcgtc cagcgccttg ccgccaagtt tgacgacgtg aaatgcgtcg
      481 agagccgggg tcgcctcgtc gagatggtcg tcgatggcgt tggcgtagcc gcggaacggg
      541 tccaaggtgg cgaccttcac cccggcgcag aactccccgc cgcggcctgc cagccagtcc
      601 ccgaacacct tcccggagcg cccagggacc aggtccagga gccgggcgcg ggtgcgtccg
      661 tcctggtcac ggctcagatc caccatcccg gtgagcatcg tggggccctt gtctctcgcg
      721 cggtgcgggg tgtggtgcca cacgtgctca tcaacgccga gggtggtgac gccgtcgaag
      781 cgggactcgt cgccagccag ctcggccagg cgaggctcga ccacccgcca gagcgtgttc
      841 caggtgacac cgagctggcg ggacaggcct tcgacggtgg cgtgctcacg gcgcagctgg
      901 ccaatcgccc accagatcgc ccgagtcgtg agcaccgccc tcgggctcgc caacgacgac
      961 aactcctcgg tgaacgtccc cgccggacag gcatcgtcgg ggcaagccca cgtccgttgc
     1021 cgccatcgga gcagcaccgc cgaacccgcg cacgggacat cacgcaacag ccgggtccgg
     1081 cgcccatgac tgagcgccac caccccgcac cgccggcacc cttgccgctc acgaggggtt
     1141 tcgatcgtca acgtgacctc ggcgccggat cggacaacag caaccacgtg gacgccgtcc
     1201 aagtcgacga gagtgtcaat gcgggaacag tacgcatcgg gcgtagcgca gcaggtcaca
     1261 gtaggcttgg tcatcgttga ggtcctcgtg gagcagtggc ttagtcacta ctgatcctga
     1321 gggcctcgac ctacgtcacc agggatcctg cacgcaccgg cgcgtcacgc tcctcccacc
     1381 gcaactctga agagccactt tacagttcct caaggaccgg atcggctgac ttgcgtacgg
     1441 gccttgcgcg cgatcgccgt tgccaccatt agctctcacg cttccgatcg cggcaatgtt
     1501 tgtccgagac caacgaacct cggcagcctt cttg
//
LOCUS       DAOSUG010000552         1528 bp    DNA     linear   ENV 10-JUN-2024
DEFINITION  MAG TPA_asm: Micropruina sp. isolate AMR_MDS_2195
            NODE_33613_length_1528_cov_3.566870, whole genome shotgun sequence.
ACCESSION   DAOSUG010000552 DAOSUG010000000
VERSION     DAOSUG010000552.1
DBLINK      BioProject: PRJNA925477
            BioSample: SAMN32864262
            Sequence Read Archive: SRR4236673
KEYWORDS    WGS; ENV; Metagenome Assembled Genome; MAG; Third Party Data; TPA;
            TPA:assembly.
SOURCE      Micropruina sp. (wastewater metagenome)
  ORGANISM  Micropruina sp.
            Bacteria; Bacillati; Actinomycetota; Actinomycetes;
            Propionibacteriales; Nocardioidaceae; Micropruina.
REFERENCE   1  (bases 1 to 1528)
  AUTHORS   Abdulkadir,N., Saraiva,J.P., Zhang,J., Stolte,S., Gillor,O.,
            Harms,H. and Rocha,U.
  TITLE     Genome-centric analyses of 165 metagenomes show that mobile genetic
            elements are crucial for the transmission of antimicrobial
            resistance genes to pathogens in activated sludge and wastewater
  JOURNAL   Microbiol Spectr 12 (3), e0291823 (2024)
   PUBMED   38289113
REFERENCE   2  (bases 1 to 1528)
  AUTHORS   Abdulkadir,N.
  TITLE     Direct Submission
  JOURNAL   Submitted (24-MAR-2023) Environmental Microbiology, Helmholtz
            Center for Environmental Research -UFZ, Permoserstr 15, Leipzig
            04318, Germany
COMMENT     The annotation was added by the NCBI Prokaryotic Genome Annotation
            Pipeline (PGAP). Information about PGAP can be found here:
            https://www.ncbi.nlm.nih.gov/genome/annotation_prok/
            
            ##Genome-Assembly-Data-START##
            Assembly Method        :: SPAdes v. 3.11.1
            Genome Representation  :: Full
            Expected Final Version :: Yes
            Genome Coverage        :: Not Applicable
            Sequencing Technology  :: Illumina MiSeq
            ##Genome-Assembly-Data-END##
            
            ##Genome-Annotation-Data-START##
            Annotation Provider               :: NCBI
            Annotation Date                   :: 05/04/2023 04:57:10
            Annotation Pipeline               :: NCBI Prokaryotic Genome
                                                 Annotation Pipeline (PGAP)
            Annotation Method                 :: Best-placed reference protein
                                                 set; GeneMarkS-2+
            Annotation Software revision      :: 6.5
            Features Annotated                :: Gene; CDS; rRNA; tRNA; ncRNA
            Genes (total)                     :: 2,776
            CDSs (total)                      :: 2,750
            Genes (coding)                    :: 2,714
            CDSs (with protein)               :: 2,714
            Genes (RNA)                       :: 26
            tRNAs                             :: 24
            ncRNAs                            :: 2
            Pseudo Genes (total)              :: 36
            CDSs (without protein)            :: 36
            Pseudo Genes (ambiguous residues) :: 0 of 36
            Pseudo Genes (frameshifted)       :: 2 of 36
            Pseudo Genes (incomplete)         :: 32 of 36
            Pseudo Genes (internal stop)      :: 2 of 36
            ##Genome-Annotation-Data-END##
FEATURES             Location/Qualifiers
     source          1..1528
                     /organism="Micropruina sp."
                     /mol_type="genomic DNA"
                     /submitter_seqid="NODE_33613_length_1528_cov_3.566870"
                     /isolate="AMR_MDS_2195"
                     /isolation_source="Activated sludge of municipal
                     wastewater treatment plants"
                     /db_xref="taxon:2737536"
                     /environmental_sample
                     /geo_loc_name="Canada"
                     /lat_lon="49.25 N 123.1 W"
                     /collection_date="2015-06-16"
                     /metagenome_source="wastewater metagenome"
                     /note="metagenomic"
     gene            complement(<1..701)
                     /locus_tag="PLF56_13875"
     CDS             complement(<1..701)
                     /locus_tag="PLF56_13875"
                     /EC_number="1.2.1.11"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_002531683.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_function: GO:0004073 - aspartate-semialdehyde
                     dehydrogenase activity [Evidence IEA];
                     GO_function: GO:0016620 - oxidoreductase activity, acting
                     on the aldehyde or oxo group of donors, NAD or NADP as
                     acceptor [Evidence IEA];
                     GO_function: GO:0051287 - NAD binding [Evidence IEA];
                     GO_process: GO:0008652 - amino acid biosynthetic process
                     [Evidence IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="aspartate-semialdehyde dehydrogenase"
                     /protein_id="HOQ54687.1"
                     /translation="MTLMRVGVFGATGTVGNVMRSLLVERDFPVDSIRFFSSARSAGR
                     QVPFKGEQITVEDTATADFAGLDIALMSAGATMSREFAPKVAAAGALVVDNSSAWRKD
                     PEVPLVVSEVNPEDAENPPKGIIANPNCTTMAAMPVLKPLHDAAGLEALQVTTFQAVS
                     GSGVKGVEALASQLGQIADPRVLAHDGSAFRPADTAPYVAPIAFNVVPLAGNLVDDGE
                     GETDEEPESIGHDVG"
     gene            773..1420
                     /locus_tag="PLF56_13880"
     CDS             773..1420
                     /locus_tag="PLF56_13880"
                     /EC_number="3.4.-.-"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_015069116.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_function: GO:0004222 - metalloendopeptidase activity
                     [Evidence IEA];
                     GO_function: GO:0046872 - metal ion binding [Evidence
                     IEA];
                     GO_process: GO:0006508 - proteolysis [Evidence IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="M23 family metallopeptidase"
                     /protein_id="HOQ54688.1"
                     /translation="MSISVDLAYPFVGAWRVRNSPANRVPSHGTRLFATAYSIDFVPV
                     DDDGGGTPFTVAALLRPQPPEQFVGFGRPVLAPLAGLVVATHAAEPDHSSYRGLPSLG
                     YALTQRHRAASGWAALAGNHVLVEHAGVIVALCHLQQGSVLVEAGQRVGVGDPLGRCG
                     NSGNSTEPHVHLQALDHPDPARAQAVPISFAGSLPRNGALVEVGRAPGPRQNQST"
BASE COUNT          233 a          555 c          530 g          210 t
ORIGIN      
        1 aaacccacgt catgcccgat gctctccggc tcctcgtcgg tctccccctc gccgtcgtcg
       61 acgaggttgc ccgccagcgg caccacgttg aacgcgatgg gcgcgacgta gggcgcggtg
      121 tccgccggcc ggaacgccga cccgtcgtgg gccaggacgc gggggtccgc gatctggccg
      181 agctgggagg ccagggcctc gacgcccttg acgcccgagc cagacacggc ctggaacgtg
      241 gtcacctgga gcgcctccag gccggcggcg tcgtgcagcg gcttgagcac cggcatggcc
      301 gccatcgtgg tgcagttggg gttggcgatg atgcccttgg gcgggttctc ggcgtcttcg
      361 ggattgacct cgctgaccac cagcggcacc tcggggtctt tgcgccaggc gctggagttg
      421 tcgacgacca gggcaccggc ggcggccacc ttgggcgcaa actcgcgcga catcgtcgca
      481 ccggccgaca tcagcgcgat gtcgagcccc gcgaagtcgg cggtggcggt gtcttcgacg
      541 gtgatctgct cgcccttgaa cggcacctgc cggccggccg agcgggccga ggagaagaac
      601 cggatcgagt cgaccggaaa gtcgcgctcg accagcaacg aacgcatcac attgcccacc
      661 gtgccggtgg cgccgaaaac tcccacacgc atgagggtca gcctatcggt gtcggccgga
      721 cgcacccgcg tccgtatcgc cgacgcccca cgccccgggc cagaatgaca cggtgagcat
      781 cagcgttgac ctggcgtacc ccttcgtcgg agcctggcgg gtgcgcaaca gcccggcgaa
      841 ccgggtgccg agccacggaa cccgcctatt cgccactgcg tactcgatcg acttcgtgcc
      901 ggtggacgac gacggcggcg gcacgccgtt caccgtggcc gcgttgctgc ggccacaacc
      961 acccgagcag ttcgtcggct tcggacgtcc ggtgctggcg ccgctcgcgg ggctggtcgt
     1021 ggcgacccac gcggccgagc ccgaccactc gagctatcgc ggcctgccct cactgggcta
     1081 tgccctcacc cagcgccacc gggccgcctc ggggtgggcg gcgctggccg gcaaccacgt
     1141 gctggtcgag cacgccggcg tcattgtcgc gctgtgccac ctgcagcagg gcagcgtgct
     1201 cgtcgaggcg ggccaacgcg tcggggtggg cgatccgctc ggccgctgcg gcaactcggg
     1261 caacagcacc gagccgcacg tgcatctgca ggccctcgac caccctgatc cggctcgcgc
     1321 ccaagcggtg ccgatcagct tcgccggatc gctgccgcgc aacggagccc tggtcgaggt
     1381 gggacgtgcg cccggcccgc gtcagaacca gtcgacgtag ggcgccagca gcgaatagcc
     1441 gacgaaggcg accaggtcga gcacgatgtg cgccaccacc agcggcccga cccgcttgaa
     1501 ccgcaggtac agcaacccga agaccagg
//
